LOCUS       DASVTM010000001         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1010842,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000001 DASVTM010000000
VERSION     DASVTM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010842"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..2213
                     /locus_tag="VF633_00005"
     CDS             228..2213
                     /locus_tag="VF633_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469470.1"
                     /translation="MGSLTRANAARSASKGAAPKPTTTKGQQIGKASLGERLKYGFDK
                     SMAAGPIALVGWLSLVTLIVIVFAASVLALSHMGPDGGFNFVEAAWQSLMRTMDAGTM
                     GGDAGWGFRILTLFVTLMGILVFSALISIISAGLDSALDSLRKGRSRVLETDHTVILN
                     WSPSIFDIISELVVANESRKKPRVVIMANKDKVEMEDEIADKVPDLKNTRIICRSGDP
                     TDLFDLSLVNAQTCRSIIVVSPEGVDDPDSQVIKTVLALTNDPKRREERYRIAAELRD
                     ARNAEVARIVGGDEVQLVLADDLISRIVVQSSRQAGLSAVYSELLDFDGCEIYTTELE
                     ELIGVTYGDAVMGYEDSALIGIRYPDGKVRMNPPMDEVIVAGSRAIIIAEDDEAIKLT
                     PMPEDAIDASAMRKPTVVKRKAERTLIIGWNRRGPMIAHELSKYVKPGSVLTVAGDTP
                     GFEEEILSMPLGSQNMKVEAVVIDTSHSASLAALDPVSYDSIMVLGYSDTMEAQSADT
                     RTLITLLHLRKLSERLGTHISVVSEMIDIRNRELAEVTRADDFVVSNKLVSLMLAQAS
                     ENEFLSSIFDDLLDEDGSEIYMRPVGDYVDLSGPLSFYTIAEAARRRGETAIGYRRKR
                     EGAEADPRNMGGVVVNPSKSEVLEYLPEDRVIVLAED"
     gene            complement(2295..2546)
                     /locus_tag="VF633_00010"
     CDS             complement(2295..2546)
                     /locus_tag="VF633_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX8469471.1"
                     /translation="MPPRHPDTIVPPPEEIVVATKRVACDGGNGIHGATLGHPLVYLD
                     MGEDDFVECSYCDRRFVLSAHAHSENEYLDPAARPPEAH"
     gene            complement(2635..2898)
                     /locus_tag="VF633_00015"
     CDS             complement(2635..2898)
                     /locus_tag="VF633_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469472.1"
                     /translation="MTKTRMIGWAAMTVCGLGYLGCAVIAVLAKTGDLDTNRALLLGA
                     VSALIGESGLWVAAGCLGLTIFKKRQALFDKVFRRRPPLANAG"
BASE COUNT          558 a          934 c          956 g          547 t
ORIGIN      
        1 gcagatagcg cattgcggct cccctgatcg cgcccgtgag gcccgtcgtc ggcggcctat
       61 agtgcgaaaa cgaggctttc taaagcgggc ggcttaggcg tattcatttc gcttcgacgt
      121 tccttaatca cttcgggtga caaggcgcgg agatcgtcgg tcagggggcc ggcggacata
      181 ttcagcgccg gcgggggaac gcacggcgtg ggggactttc cgagaccatg ggatcattga
      241 cccgcgcaaa cgcggcgcgc agcgcgtcca agggcgccgc gcccaaaccc accacgacca
      301 aaggccagca gatcggcaag gcgagcctcg gcgagcgcct gaagtacggc ttcgacaagt
      361 cgatggccgc aggcccgatc gcgctggtcg gctggctcag cctggtgacc ctgatcgtca
      421 tcgtgtttgc ggcttcggtg ctggctctgt ctcatatggg tcctgatgga ggcttcaact
      481 tcgtcgaggc tgcctggcaa tcgctgatgc gaacgatgga cgccggcacg atgggtggcg
      541 acgcgggctg gggtttccgc atcctcacgc tgttcgtgac cctgatgggc attctggtgt
      601 tttcggcttt gatctcgatc atctcggccg gcctcgactc ggcgctggac agcctgcgca
      661 agggccggtc gcgcgtgctg gagaccgatc acaccgtcat cctgaactgg tcgccgtcga
      721 tcttcgacat catctccgaa ctggtcgtcg ccaacgagag ccgcaagaaa ccgcgtgtcg
      781 tcatcatggc caacaaggac aaggtcgaga tggaggacga gatcgcggac aaggtccccg
      841 acctcaagaa cacgcgcatc atctgccggt cgggtgaccc aaccgacctg ttcgacctgt
      901 cgctagtcaa tgcccagacc tgccgctcca tcatcgtggt ctcgcccgaa ggcgtggacg
      961 atccggacag ccaggtcatc aagaccgttc tggcgcttac caacgatccc aagcgccgcg
     1021 aggaacgcta tcgcatcgcc gccgagctgc gcgacgccag gaacgccgaa gtcgctcgca
     1081 tcgttggcgg agacgaggtc cagctggtgc tggccgacga cctgatctcc cgcatcgtcg
     1141 tgcagtcgag ccgtcaggcc ggcctgtcgg cggtctattc cgagctgctc gatttcgacg
     1201 gctgtgaaat ctacacgacc gagctggagg agctgatcgg cgtcacctat ggcgatgccg
     1261 tcatgggcta tgaagactcc gccctgatcg ggatccgtta cccggacggc aaggtgagga
     1321 tgaatccgcc gatggacgag gtcatcgtcg ccggttcgcg cgcgattatc atcgccgagg
     1381 acgacgaggc catcaaactg acgccaatgc cggaagacgc catcgacgcc tcggccatgc
     1441 gcaagccgac cgtggtcaag cgcaaggcgg aacgcaccct gattatcggc tggaaccgtc
     1501 gcggtcccat gatcgcccat gagctgtcca aatacgtgaa gccgggctcg gtcctgaccg
     1561 tcgccggcga cactccgggc ttcgaggagg aaatcctgtc gatgccgctg ggctcgcaga
     1621 acatgaaggt cgaggccgtc gttatcgaca ccagccattc agcctcgctc gcggcgctgg
     1681 atccggtctc ctacgactcg atcatggtgc tgggttattc ggatacgatg gaggcccagt
     1741 cggccgacac gcggacgctg atcaccctct tgcacctcag gaagctgtcg gaacggctcg
     1801 gcacccacat cagcgtggtc tccgagatga tcgacattcg taaccgcgag ctggccgagg
     1861 tcactcgcgc cgacgatttc gtggtgtcca acaaactggt cagcctgatg ctggcccagg
     1921 cgtccgagaa cgagttcctg tcctcgatct tcgacgacct gctggatgag gacggttcgg
     1981 aaatctacat gcgcccggtc ggcgactatg tggatctgtc tggcccgctc agcttctaca
     2041 ccatcgccga ggccgcccgt cggcgcggcg agaccgccat cggttatcgc cgcaagcgcg
     2101 agggggccga ggccgaccct cgcaacatgg gcggcgtcgt ggtcaatcca tccaagtcgg
     2161 aagttctgga atacctgccg gaagaccggg tcatcgtcct cgcggaggac tgatcccgcg
     2221 ccgggcttga aatgaagagg gtcggcttcc gatccggaag ccgccccttt ttcgattgtg
     2281 atgtcggctg aagcctagtg tgcttcagga ggccgtgccg cagggtcgag gtattcattc
     2341 tcgctgtggg cgtgcgccga aaggacgaag cggcggtcgc agtagctgca ctcgacgaaa
     2401 tcgtcctcgc ccatgtcgag atagaccagc ggatggccca aggtcgcgcc gtggatgccg
     2461 ttgccgccgt cgcaggcgac acgcttggtg gcgaccacga tctcttcggg gggagggacg
     2521 atggtgtcgg ggtggcgcgg gggcatgctg aaagcctgaa aagggaggtg tccgttcact
     2581 aggcgggcga acccgcgtcg tcaaactctt cccccgtctg gctttccggt cgcgtcagcc
     2641 tgcgttcgcg agcggcgggc gacggcggaa gaccttgtca aacagggcct gtcgcttctt
     2701 gaagatcgtc aggccgaggc agcctgcggc aacccaaagg ccgctctcac caatgagggc
     2761 ggatacggcg cccagcagca gcgcccggtt cgtgtcgagg tcaccagtct tggccagcac
     2821 tgcgatcacg gcgcagccca gatagccgag gccgcaaacc gtcatcgcgg cccaaccgat
     2881 catccgagtc tttgtcataa cgtatctccg tcaagggtgg ttgtctcaat gacaagcaaa
     2941 ctggacacca agcgcaccta cgtcaagcgt cattgacata aagtacgtgt cgctt
//
LOCUS       DASVTM010000002        10196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_904515,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000002 DASVTM010000000
VERSION     DASVTM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904515"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF633_00020"
     CDS             <1..362
                     /locus_tag="VF633_00020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX8469473.1"
                     /translation="YLPLAVIIAGILLLEMIVITSAVISGGVAADATVPVAATLQASN
                     IETIGRVLYTDYVYFFQAAGIVLLIAMIGAIVLTLRHKPGVRRQNIHDQVTRDAKTAI
                     EIKGAVTGAGVTPEELL"
     gene            362..667
                     /gene="nuoK"
                     /locus_tag="VF633_00025"
     CDS             362..667
                     /gene="nuoK"
                     /locus_tag="VF633_00025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX8469474.1"
                     /translation="MVGLQAYLVVAAMLFTIGVFGIFVNRKNIIVILMSIELILLAVN
                     INFVAFSAYLHDVQGQIMAMFVLTVAAAEAAVGLAILVTFFRNRGDIAVDDASVMKG"
     gene            670..2760
                     /gene="nuoL"
                     /locus_tag="VF633_00030"
     CDS             670..2760
                     /gene="nuoL"
                     /locus_tag="VF633_00030"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX8469475.1"
                     /translation="MNFELLVTLGVFAPLLGAAIVGFFGRRLGDLVSQTITTGLLFFA
                     CAIAWIVFSAWTWGGLEAFTVRLAPFITVGDFQSIWSIRIDALSATMLVVVTTVSSLV
                     HLYSWGYMSEDDSRPRFFAYLSLFTFAMLALVTAADFMQLFFGWEGVGLASYLLIGFW
                     YKKPTASAAAIKAFVVNRVGDFGFALGIITIFWMFGTIDFAELFPMIASKAGTTWEFL
                     GHNWSAIDLAAFLLFIGAMGKSAQFFLHTWLPDAMEGPTPVSALIHAATMVTAGVYML
                     CLLSPIFEYAPGASLIVAVIGAITALFAATVGMMQNDIKRVIAYSTCSQLGYMFFAAG
                     VGAYQGAMFHLFTHAFFKALLFLGAGSVIHGMHHEQDMRKMGGLWKLMPITYAVMVIG
                     TIAITGLGIPEVGGFAGFYSKDSIIESAYAAASSGHSPVGYFAFFIGVLAAGLTSYYS
                     WRLIFMTFHNKPVWKDEHAHDDHAHAPALAHDDHASHAQLETHSEPEGHDEHHAHGPL
                     KPHESPWVMVVPLLLLSVGAVFAGMVFAPNFIGHGEHEFWRGAIFTGEHNHVLHESHE
                     VPMWVKLSPLVLTILGALIAFWIYVLKEGVAKLWADAQSPVWAFMYNKWYFDELYDFV
                     FVKGAKALGDFFWKIGDVKIIDGWGPNGAAWSSLKSAGWLSKFQSGYVYYYAFVMLVG
                     VAGFLAFALYAFGA"
     gene            2763..4247
                     /locus_tag="VF633_00035"
     CDS             2763..4247
                     /locus_tag="VF633_00035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX8469476.1"
                     /translation="MPAILSIVTFLPLVGALLVLAARFTQKSADAAAPMARWIALGTT
                     VVTLAVSVLLTAQFDQSNTAYQFVEMHTWFAGASYHLGVNGISILFVLLTAFLLPICI
                     LASWKSVETRVVDYMIAFLVLETLVIGVFTSLDLFLFYIFFEGTLVPMFIIIGVWGGA
                     NRIYASYKFFLYTLLGSVLMLLAMLWMAYYAGTTSIPELQAFQFPAQFQPLLWLAFFA
                     SFAVKMPMWPVHTWLPDAHVQAPTAGSVILAGILLKLGGYGFILFNVPMFPIASQMFQ
                     PLVMTLSVIAIVYTSLVAFRQTDMKKLIAYSSVAHMGFVTMGIFAGNAQGMQGAVFQM
                     LSHGLISSALFLCVGVIYDRMHTREISFYGGLTSRMPWYAAIFLMFTMANVGLPGTSG
                     FVGEILTMTGGYQAATWVAFVAASGVIFSAVYALTLYRNVMFGEITNPALAAITDIDK
                     RELLIFVPLIIGTIWLGVQPDLVLNYTAASVDAVVGAYRAAIGG"
     gene            4255..5700
                     /gene="nuoN"
                     /locus_tag="VF633_00040"
     CDS             4255..5700
                     /gene="nuoN"
                     /locus_tag="VF633_00040"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX8469477.1"
                     /translation="MPDLSSALHLAAPELTLAIGTLILLVLGAFLGEKSARMISILAV
                     ALLVGASAVVANGPLGAAFHGAYVADPLAVYAKVMIYLSAAVAIILGEGWMRRTGIAK
                     FEFPVLIVLASVGMSMMASSGDLISLYVGIELHSLALYVLAAFHRDNLKASEAGLKYF
                     VLGALSSGLLLYGASLIYGFTGSMRFADIAEVASRGDVGPGLIFGLVFLISGLAFKVS
                     AAPFHMWTPDVYEGAPTPVVALFATAPKVAAMVLIARVLDGPFIGIHDQWAQVLVLIA
                     LLSFAVGSFGGLAQKDIQRLLAYSSIINIGYALLAIAAGTTVGVQALLMFMTLYVIDQ
                     FGFFSILLSLSKNGKPIRKISDLAGLKKTRPVTAIALTVLSLSVLGMPPFSGFWAKYY
                     VFGAAASSGYWMVAAAGLVASVVAAFYYLRIIKLMWFDAPLDEVATDKAPAEALSIAW
                     ICAAFSFPLVIVALTFLEPLTKAAAIGFGAG"
     gene            5705..6466
                     /locus_tag="VF633_00045"
     CDS             5705..6466
                     /locus_tag="VF633_00045"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX8469478.1"
                     /translation="MTPSILLLEETDSTNAEARRRAEAGEAGPLWIAARRQTAGRGRR
                     GREWVSETGNLAATLLLTTRKAPAEAAQITFVAALAAADLVCTFVPAPLVTIKWPNDV
                     MIGADKAVGILVESGAHENGLLWLAVGIGVNLSDAPSGAERPATSIAGHLKGDVHAAP
                     KPEAAAEILAAHFAIWMDRWETLGFQVVLDAWVERSRGIEGPCTARLGHETISGTAEG
                     VAADGALRLRLVDGTLRLISAGDVFFGAPAGKEPA"
     gene            6463..7242
                     /locus_tag="VF633_00050"
     CDS             6463..7242
                     /locus_tag="VF633_00050"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX8469479.1"
                     /translation="MMLLAIEQGNTNTLFAVHDGESWIAQWRTATEANRTADEYAVWL
                     TQLLQMHSLSFADLHGCIISSVVPQSLFNLRNLARRYLNNEPLVIGENAQLGIEVRIT
                     KPSEAGADRLVNAIGAHLIYPGDLLLIDSGTATTFDIVAADGAFEGGAIAPGINLSLQ
                     ALHEAAAKLPRIAIQKPDRVIGKDTVECMQSGVFWGYIALIEGMVVRMKAEWGAPLTV
                     VGTGGVASLFEGSTKFIDRFDPDLTIRGLLEIWRRNTALKD"
     gene            7243..8919
                     /locus_tag="VF633_00055"
     CDS             7243..8919
                     /locus_tag="VF633_00055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX8469480.1"
                     /translation="MKKSANDELVFLPLGGSNEIGMNLNCYGYGPEHDRQWLIADVGV
                     TFGDPSTPGIDVIVPDPSFLEGEKIRGIVLTHAHEDHIGALGWLWTRIKAPLYATPFT
                     AFLIREKLREAGILNQVKIHEVPLNGTIDLDPFKVTMITMTHSIAEPNGLAIRTPLGT
                     VFHTGDWKIDDGPVIGEVTDGAAITALGDEGILAMVCDSTNVFSEGFAGSESGVAEEL
                     DTLIGSLKTGRIAVGCFASNVARMDSVIRAAEKHGRRIFLAGRSMHRITAAAKHVGML
                     SDVKKFLSDDEARVWPADEILYLCTGSQGEQNAALARVASGTHPTVKLGRGDHCIFSA
                     RIIPGNELGIGVLQDRLSERGVRLYTEKDHPGIHVSGHPCRGELRQMYAWARPEIAIP
                     VHGMRRHIAEHALLATELGVPQAVTPRNGDMVRLAPGPAVIIDEVPNGRLFIDGGMLV
                     PEQGEALRERRHASTNGILVVSFAMDRRGKVVSDIDVRGIGLPGDDESPLGDALDHLA
                     ERAEQAINSLKGDAREDDTVVEQAVARVLKKASQQIWDRRPIVETVILRL"
     gene            9022..9600
                     /locus_tag="VF633_00060"
     CDS             9022..9600
                     /locus_tag="VF633_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469481.1"
                     /translation="MSVRSLAVFAVPALATLAIAATALSGCDDGKGVRTESTKVTVNG
                     TPKAGYVAPSGQQAPAGDFQTNVDLVKSLYALDAIPTEPAEIGKYFAAEFITPMAPTE
                     DGVTVDFDYRINGQDGAADGLTIEETDGDGANGRVVSKFTNMGHAEEIHWTVCRQPNG
                     TLRLMDVRNAATGDDAWDMRAIVGLPARPEGC"
     gene            9677..9928
                     /locus_tag="VF633_00065"
     CDS             9677..9928
                     /locus_tag="VF633_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEX8469482.1"
                     /translation="MPIGPITMFAIYITIWWTVLFAVMPLGMSQEDQAPPTDGGQWGA
                     PKTPNLKKKFITTTWVAAIVWAFGMVIIFTGWLPLPKIG"
     gene            10018..>10196
                     /locus_tag="VF633_00070"
     CDS             10018..>10196
                     /locus_tag="VF633_00070"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517381.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX8469483.1"
                     /translation="MRLSRYFMPTLKEAPSDAQIVSHKLMLRAGMIKQEAAGIYAGLP
                     LGLRVLERIEQIVRE"
BASE COUNT         1613 a         3541 c         3059 g         1983 t
ORIGIN      
        1 gttacctgcc gctggcggtg atcatcgcgg gcatcctgct gctggagatg atcgtgatca
       61 cctcggcggt catctcgggc ggcgtcgcag cggacgcgac ggtcccggtg gcggccaccc
      121 tgcaggcctc gaacatcgag accatcggcc gggtgctgta taccgactac gtctacttct
      181 tccaggcggc ggggatcgtg ctgctgatcg ccatgatcgg cgccatcgtc ctgacgctgc
      241 gtcacaagcc gggcgttcgt cgtcagaaca tccacgatca ggtcacgcgt gacgccaaga
      301 ccgcgatcga gatcaagggc gccgtcaccg gcgccggcgt cacccccgag gagcttctct
      361 gatggtcggt ctgcaggcct atctggtggt cgccgccatg ctgttcacca tcggggtgtt
      421 cggcatcttc gtgaaccgca agaacatcat cgtcatcctg atgtcgatcg agctcatcct
      481 gctcgccgtg aacatcaatt tcgtcgcttt ctcagcctat ctgcacgacg tgcaggggca
      541 gatcatggcg atgttcgtcc tgaccgtcgc cgccgctgag gccgccgtcg gcctcgccat
      601 cctggtgacc ttcttccgca accgcggcga catcgcggtt gatgacgcct cggtcatgaa
      661 gggctgatcg tgaacttcga actgctcgtt acactcggcg ttttcgctcc gcttctgggc
      721 gccgccatcg tcggcttctt cggtcgccgt ctgggcgatc tcgtctccca gacgatcacg
      781 accggcctgc tgttcttcgc ctgcgccatt gcctggatcg ttttcagcgc ctggacctgg
      841 ggtgggctcg aagccttcac cgtgcgtctg gcgcccttca tcactgtcgg cgacttccag
      901 tcgatctggt cgatccgcat cgacgccctg tcggcgacca tgctggtcgt ggtcacgacg
      961 gtgtcgtcgc tcgtccacct ctatagctgg ggctatatgt cggaagacga cagccgcccg
     1021 cgcttcttcg cctatctgtc gctgttcacc ttcgccatgt tggcgctggt caccgccgcc
     1081 gacttcatgc agctgttctt cggctgggag ggcgtcggcc tcgcgtccta tctgctgatc
     1141 ggcttctggt acaagaaacc gaccgcctcg gccgccgcca tcaaggcctt cgtcgtcaac
     1201 cgggtcggcg acttcggctt cgcgctcggc atcatcacca tcttctggat gttcggcacg
     1261 atcgacttcg ccgagctgtt cccgatgatc gcgtccaagg ccggcacgac ctgggagttc
     1321 ctcggccaca actggtcggc tatcgatctg gcggcgttcc tgctgttcat cggcgcgatg
     1381 ggcaagtcgg cccagttctt cctgcacacc tggcttcccg acgccatgga aggcccgacg
     1441 cccgtgtcgg cgctgatcca cgcggccacc atggtcaccg ccggcgtcta catgctctgc
     1501 ctgctgtcgc cgatcttcga gtatgcgccc ggcgcatcgc tgatcgtcgc cgtcatcggc
     1561 gccatcacgg cgctgttcgc tgcgaccgtc ggcatgatgc agaacgacat caagcgggtc
     1621 atcgcctatt cgacctgttc gcagctcggc tacatgttct tcgccgccgg tgtcggcgcc
     1681 tatcagggcg cgatgttcca cctcttcacg cacgccttct tcaaagccct gctgttcctc
     1741 ggcgccggtt cggtgatcca cggcatgcac cacgagcagg acatgcggaa gatgggcggc
     1801 ctgtggaagc tgatgccgat cacctatgcc gtcatggtca tcggcacgat cgccatcacc
     1861 ggtctgggta tccccgaagt gggcggtttc gccggcttct attccaagga ctcgatcatc
     1921 gagagcgcct acgcggcggc ctcgagcggt cattcgcccg tcggctattt cgccttcttc
     1981 atcggcgtcc tcgccgctgg tctgacgagc tactattcgt ggcgcctgat cttcatgacc
     2041 ttccacaaca agcccgtctg gaaagacgag catgcgcatg acgaccacgc gcacgccccg
     2101 gctctcgctc atgacgacca cgcctcgcac gcccagctcg agacccattc ggaaccagaa
     2161 ggccacgacg agcatcacgc tcacggcccg ctgaagccgc acgaaagccc atgggtcatg
     2221 gtcgtgccgc tgctgctgct atcggtcggc gccgtgttcg ccggtatggt cttcgccccg
     2281 aacttcatcg gtcacggcga gcacgagttc tggcgcggcg ccatcttcac cggcgagcac
     2341 aaccacgtcc tgcacgagag ccacgaggtt ccgatgtggg tcaagctgtc gccgctggtg
     2401 ttgacgatcc tcggagccct gatcgccttc tggatctatg tgctcaagga aggcgtcgcc
     2461 aaactgtggg ccgacgccca gagccccgtc tgggcgttca tgtacaacaa gtggtacttc
     2521 gacgagctgt acgacttcgt cttcgtcaag ggcgccaagg cgctgggcga cttcttctgg
     2581 aagatcggcg acgtcaagat catcgacggt tggggtccta acggcgccgc ctggtcctcg
     2641 ctcaagtcgg ccggctggct atctaaattc cagtccgggt acgtctacta ttatgcgttc
     2701 gtgatgttgg tcggcgtcgc cggtttcctc gccttcgccc tctatgcgtt cggagcctga
     2761 cagtgcccgc aatcctgagc atcgtcactt tcctgccgct ggttggagct ctgctcgtgc
     2821 tggccgcacg gttcacgcag aagtccgctg atgcggctgc gcccatggct cgctggatcg
     2881 cgctcgggac cactgtggtc accctggcgg tgtcggtcct gctgacggcg cagttcgatc
     2941 agtcgaacac cgcctatcag ttcgtcgaga tgcacacctg gttcgccggg gccagctatc
     3001 atctgggcgt caacgggatc tcgatcctgt tcgtcctgct gacggccttc ctgctgccga
     3061 tctgcattct ggccagctgg aagtcggtcg agacgcgcgt cgtcgattac atgatcgcgt
     3121 tcctcgtgct ggagacgctg gtcatcggcg tgttcacgtc gctggacctg ttcctgttct
     3181 acatcttctt cgaaggcacg ctggtgccga tgttcatcat catcggcgtg tggggcgggg
     3241 cgaaccggat ctacgcctcg tataaattct tcctctacac cctgctgggg tcggtgctga
     3301 tgctgctggc catgctgtgg atggcctatt acgcgggcac caccagcatt cccgaactgc
     3361 aggccttcca gttcccggcc cagttccagc cgcttctgtg gctggccttc ttcgcctcgt
     3421 tcgcggtcaa gatgccgatg tggccggtcc acacctggct gcccgacgcc cacgttcagg
     3481 cgccgacggc gggctcggtc attctggccg gcatcctgct gaagctgggg ggctacggct
     3541 tcatcctgtt caacgtgccg atgttcccca tcgcgtcgca gatgttccag ccactggtca
     3601 tgaccctgtc ggtcatcgcc atcgtctaca cctcgcttgt cgccttccgt cagacggaca
     3661 tgaagaagct gatcgcctat tcgtcggtcg cgcacatggg cttcgtgacc atgggcatct
     3721 tcgccggcaa cgcgcagggc atgcagggcg ccgtgttcca gatgctgagc cacggcctga
     3781 tttccagcgc gctcttcctt tgcgtcggcg tcatctacga ccggatgcac acccgcgaga
     3841 tcagcttcta cggcggcctg acctcgcgca tgccctggta cgcggcgatc ttcctgatgt
     3901 tcaccatggc caacgtcggc ctgccgggca cctcgggctt cgtcggtgaa atcctgacca
     3961 tgaccggcgg ctatcaggcc gcgacctggg tggcgttcgt cgccgcctcg ggcgtgatct
     4021 tctcggcggt ctatgcgctg acgctgtatc gcaacgtcat gttcggcgag atcaccaacc
     4081 cggcccttgc cgccatcacg gacatcgaca agcgcgagct gctgatcttc gtgcccctga
     4141 tcatcgggac aatctggctg ggcgttcagc ccgacctcgt gctgaactac accgccgcct
     4201 ccgtcgacgc cgtcgtgggt gcctaccgtg ccgcgatcgg cgggtgagac cataatgcct
     4261 gatctgtcct ccgcccttca tctcgccgcg ccggagctca cgctcgccat cggcaccctg
     4321 atcctgctcg tgctcggcgc cttcctcggc gagaagagcg cgcgcatgat ctcgatcctt
     4381 gcggtcgccc tgctcgtcgg ggcctcggcc gtcgtcgcca acggcccgct cggcgcggcc
     4441 ttccacggcg cctatgtggc cgatccgctg gccgtctacg ccaaggtcat gatctatctg
     4501 tccgccgccg tcgccatcat cctcggcgaa ggctggatgc ggcgcaccgg catcgccaaa
     4561 ttcgagttcc cggtcctgat cgtgctggct tcggtcggca tgagcatgat ggcctcgtcg
     4621 ggcgacctga tctcgctcta cgtcggcatc gagcttcact cgctggcgct ttacgtcctc
     4681 gccgccttcc accgcgacaa ccttaaggcc tcggaagccg gcctgaagta tttcgtcctc
     4741 ggcgcgctgt cgtccggcct gctgctgtac ggcgccagcc tgatctacgg cttcaccggc
     4801 tcgatgcgct tcgccgacat cgccgaagtc gcctcgcgtg gagacgtcgg ccccggtctg
     4861 atcttcggcc tcgtgtttct gatctcgggt ctggccttca aggtctcggc cgcgccgttt
     4921 cacatgtgga cgcccgacgt ctatgaaggc gccccgaccc cggtcgtcgc cctgttcgcc
     4981 acggccccca aggtcgcggc catggtcctg atcgcgcgcg ttctcgacgg cccgttcatc
     5041 ggcatccacg accagtgggc gcaggtgctg gtgctgatcg cgctgctctc gtttgccgtc
     5101 ggttcgttcg gcggtctggc ccagaaggac atccagcgcc tgctggccta ttcctcgatc
     5161 atcaacatcg gctacgccct gctggccatc gcggctggca ccacggtcgg cgtccaggcc
     5221 ttgctgatgt tcatgaccct gtacgtcatc gaccagttcg gcttcttctc gatcctgttg
     5281 tcgctatcga agaacggcaa gccgatccgc aagatctccg acctcgccgg actcaagaag
     5341 acccgcccgg ttacggccat cgcgttgacc gtcctgtcgt tgtcggtgct cggcatgccg
     5401 ccgttctcgg gcttctgggc caaatactat gtgttcggcg cggcggcgag ctcgggctac
     5461 tggatggtag cggcggcagg cctcgtggcc tcggtcgtgg cggccttcta ctacctgcgc
     5521 atcatcaagc tgatgtggtt cgacgcgccg ttggacgagg tcgctaccga caaggcgccg
     5581 gccgaagcgc tgtcgatcgc ctggatctgc gccgccttct cgttcccgct cgtgatcgtc
     5641 gccctgacct tcctggagcc cctgaccaag gctgcggcca tcggcttcgg agccgggtag
     5701 ggcggtgacg ccttccatcc tgctgctcga agagacggac tccaccaacg ccgaagcccg
     5761 tcgccgggcc gaggcgggcg aggccggtcc cctgtggatc gccgcccgcc gccagaccgc
     5821 cggtcgcggt cgtcgtgggc gtgagtgggt gtccgagacc ggcaacctcg ccgccaccct
     5881 gctgttgacg acgcgtaaag ccccggcgga agccgcccag atcaccttcg tcgccgcgct
     5941 cgccgccgcc gatctggtct gcaccttcgt ccccgcgccg ctggtgacga tcaaatggcc
     6001 caacgacgtg atgatcggcg ctgacaaggc cgtcggcatc ctcgtcgagt ccggcgccca
     6061 cgagaacggc ctgctctggc tggcggtcgg catcggcgtc aatctgagcg acgcgccctc
     6121 gggcgccgaa cgccccgcca cctcgatcgc cggtcacctg aagggcgatg tccacgcggc
     6181 cccgaagcct gaagccgccg ccgaaatcct ggccgcgcat tttgcgatct ggatggaccg
     6241 ctgggagacg ctcggctttc aggtcgtcct cgacgcctgg gtcgagcgca gccgaggcat
     6301 cgagggtccc tgcacagccc gactgggcca tgaaaccatt tccggcacag ccgagggcgt
     6361 ggccgccgac ggcgccctgc gcctgcgcct cgttgacggg accctgcgcc tcatctccgc
     6421 cggagacgtc ttcttcggag ccccggcggg caaggaaccg gcctgatgct gctggcgatc
     6481 gaacagggca acaccaacac gctgttcgcg gtccatgacg gcgagagctg gatcgcccag
     6541 tggcgcacgg ccaccgaggc caatcgcacc gccgacgaat acgccgtctg gctgacccaa
     6601 ctgctgcaga tgcacagcct cagcttcgcc gacctgcacg gctgcatcat ctccagcgtc
     6661 gtgccgcagt cgctgttcaa cctgcgtaat cttgctcgcc gctacctcaa caacgagccg
     6721 ctggtgattg gcgagaacgc tcagctcggc atcgaagtgc ggatcaccaa gccgtcggaa
     6781 gccggcgccg accgtctggt caacgccatc ggcgcgcacc tgatctaccc cggcgacctg
     6841 ctcttgatcg acagcggcac ggccaccaca ttcgacatcg tggccgctga cggcgctttc
     6901 gaaggcggtg cgatcgcacc gggaatcaac ctctccctgc aagccctgca cgaggccgcc
     6961 gcaaaactgc cccgtatcgc catccagaag ccggatcgtg tgatcggcaa ggacacggtg
     7021 gagtgcatgc aatcgggtgt cttctggggc tatatcgccc tcatcgaggg catggtcgtg
     7081 cgcatgaagg ccgagtgggg cgcgccgctc accgtcgtcg gcacgggggg cgtggcctcc
     7141 ctgttcgaag gctcaacgaa attcatcgac cggtttgatc cggacctgac catccgcgga
     7201 ctgctggaaa tctggcgccg caatactgca ttgaaagact gaatgaaaaa atccgctaac
     7261 gacgaactcg ttttcctgcc gctcggcggc tcgaacgaga tcggcatgaa cctgaactgc
     7321 tacggctacg gcccggagca tgaccgccag tggctgatcg ccgacgtcgg cgtcaccttc
     7381 ggcgatccgt cgacgccggg catcgacgtc atcgtccccg atccgtcctt cctcgagggc
     7441 gagaagatcc gcggcatcgt cctgacccac gcgcacgaag accacatcgg cgcgctcggc
     7501 tggctctgga cccgcatcaa ggcgccgctt tacgccacgc cgttcacggc cttcctgatc
     7561 cgcgagaagt tgcgcgaagc gggcatcctg aaccaggtca agatccacga agtgcctctg
     7621 aacggcacca tcgatctgga tccgttcaag gtgaccatga tcaccatgac gcactcgatc
     7681 gccgagccga acggcctggc catccgcacg ccgctgggca ccgtgttcca caccggcgac
     7741 tggaagatcg atgacggccc ggtcatcggc gaagtcaccg acggcgccgc catcaccgcg
     7801 ctcggcgacg agggcatcct cgccatggtc tgcgactcga ccaacgtctt ctccgaaggc
     7861 ttcgccggct ccgaatcggg cgttgccgaa gaactcgaca ccctgatcgg cagcctcaag
     7921 accggccgta tcgccgtcgg ctgtttcgcc tccaacgtcg cccgcatgga cagcgtcatc
     7981 cgcgccgctg aaaagcacgg ccgtcgcatc ttcctcgccg gccgctcgat gcaccgcatc
     8041 accgccgccg cgaagcatgt cggcatgctg tcggacgtga agaaattcct gtccgacgac
     8101 gaggcccgcg tctggcccgc agacgagatc ctgtatctct gcaccggcag tcagggcgaa
     8161 cagaacgcag cgctcgcccg cgttgcgtca ggcacccacc cgacggtcaa gctgggtcgc
     8221 ggcgatcact gcatcttctc ggcccggatc atccccggca acgagctcgg catcggcgtt
     8281 ctgcaggacc gtctctcgga gcggggcgtg cgcctctaca ccgagaagga ccaccccggc
     8341 atccacgtct cgggtcaccc ctgccgcggc gaactgcgcc agatgtacgc ctgggcccgc
     8401 ccggagatcg ccatcccggt ccacggcatg cgccgccaca tcgccgaaca cgccctgctg
     8461 gcgaccgagc tcggcgtccc tcaagccgtg accccgcgca atggcgacat ggtgcgtctg
     8521 gcccccggac ctgcggtcat catcgacgag gtgcccaacg gtcgcctgtt catcgacggc
     8581 ggcatgctgg tgcccgaaca gggcgaagcc ctgcgcgagc gtcgtcacgc ctcgaccaac
     8641 ggcatcctcg tggtgtcgtt cgccatggac cgtcgcggca aggtcgtctc ggacatcgac
     8701 gtgcgcggga tcggtctgcc cggcgatgac gaaagccccc tcggcgacgc cctcgaccat
     8761 ctggccgagc gtgccgaaca ggccatcaac agcctgaagg gcgacgcccg tgaggacgac
     8821 accgtagtcg aacaggccgt ggcgcgcgtg ctcaagaagg cctcgcagca gatctgggat
     8881 cgccgtccca tcgtcgagac ggtcattctc cgcctctagg tcgaagaaga cttgaagtga
     8941 gacgggcgcg gcctaatgtc gcgtccggtt ccctcgggga atgttcgacg ggtccagccc
     9001 gtcttcgggg gaagtcagcc tatgtccgtt cgatccttag ccgtcttcgc ggttccagcc
     9061 ttggccactc tcgccatcgc agcaaccgcc ttgtccggct gtgacgatgg caagggcgtc
     9121 cggaccgaat cgaccaaggt cacggtcaac ggcacgccca aggcaggata tgtcgcgccc
     9181 tcaggccagc aagcgcctgc cggtgatttc cagaccaacg tcgatctggt caaatccctt
     9241 tacgccctcg acgccatccc gaccgaaccg gccgagatcg gcaaatattt cgccgccgag
     9301 ttcatcacgc cgatggcccc gaccgaggac ggcgtcaccg tcgacttcga ctaccgcatc
     9361 aacggtcagg acggcgccgc cgacggcctc accattgagg agaccgacgg cgacggcgcg
     9421 aacggccgtg tcgtgtccaa attcaccaac atgggccacg ccgaagagat ccactggacc
     9481 gtctgtcgcc agcccaacgg cacactgcgc ctgatggacg tcaggaacgc cgctaccggc
     9541 gacgacgcct gggacatgcg cgccatcgtc ggtcttccgg cccgcccgga aggctgctga
     9601 gtctggttca ggccgacaaa ccggcgtagg gctcagcccg atccttccca ggactctacg
     9661 ctttcaggac ttcgcgatgc ccatcggccc gatcaccatg ttcgccatct acatcaccat
     9721 ctggtggacg gtgctgttcg ccgtgatgcc actggggatg tcgcaggagg atcaggcccc
     9781 gcccaccgac ggcggccagt ggggcgcacc gaagacgccg aacctgaaga agaaattcat
     9841 caccacaacc tgggtcgcgg ccatcgtctg ggcttttggc atggtgatca tcttcaccgg
     9901 ctggttgccg ctgccgaaga ttgggtaagg gttctttccc caaaggaggg gaccgcctag
     9961 cttttgcccc gtccacccgg ctaaagcaca ggtcgaactg accaagcgga ttctgccatg
    10021 cgcctgtcgc gctatttcat gcctacgctc aaagaagcgc cttccgacgc ccagatcgtc
    10081 tcgcacaagc tgatgctgcg agcggggatg atcaaacagg aggccgccgg tatctacgcc
    10141 gggctcccgc tgggtctgcg ggtgcttgaa cgcatcgagc agatcgtgcg cgagga
//
LOCUS       DASVTM010000003         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_638970,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000003 DASVTM010000000
VERSION     DASVTM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638970"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..575
                     /locus_tag="VF633_00075"
     CDS             102..575
                     /locus_tag="VF633_00075"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX8469484.1"
                     /translation="MPETPVIYVLNGPNLNLLGVREPDIYGYETLADVQALCEKAAEG
                     HTVVFRQSNHEGDLIDWIQEARTEASALVINPAGYGHTSVALLDALKTLSIPIIECHL
                     SNPATRERFRHKTYVSLVATGVVAGFGSQSYELAVRAAVKLVAERAAAATRSSQG"
     gene            595..1110
                     /gene="accB"
                     /locus_tag="VF633_00080"
     CDS             595..1110
                     /gene="accB"
                     /locus_tag="VF633_00080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX8469485.1"
                     /translation="MADPKSTPAKLNNDVEIDAALVRQLADILNDTSLTEIEVERGEL
                     RIRVAREITAAPVMQYAAAPQFAAQPQAAPAAAAPVSMPSDPATIVAKAGETVKSPMV
                     GTAYLQASPEAAPFIKIGDTVKKGQTLVIVEAMKTMNPIQAPRDGVVAEILVGDAQPV
                     EYGEPLVLLEN"
     gene            1119..2471
                     /gene="accC"
                     /locus_tag="VF633_00085"
     CDS             1119..2471
                     /gene="accC"
                     /locus_tag="VF633_00085"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8469486.1"
                     /translation="MFTKVLIANRGEIALRIHRACKEMGISTVAVHSEADRGAMWVRL
                     ADESVCIGPAPAAKSYLNIPSIIAAAEITGAQAIHPGYGFLSENARFAEIVEAHGMSF
                     IGPQPEHIRVMGDKISAKKTVLAAGIPCVPGSDGEVETVEDAIEASKSIGFPLIVKAA
                     AGGGGRGMKVALTADDLVEAVQTAQTEAKAAFGNGAVYMERYLQKPRHIEIQVIADSH
                     GNVVHLGERDCSLQRRHQKVLEEAPSPALSAEGRKAIGETVNKAIAAIGYLGVGTIEF
                     LWEDGEFFFIEMNTRLQVEHPVTEAITGVDLVREQIRIAAGLPLSFTQNDIHFEGHAI
                     EVRINAENAETFTPSPGTITDFHAPGGLGVRLDSAIYAGYSIPPYYDSLIGKLIVHGR
                     DRAECIARLKRSLNEMVIGGIDTTIPLFQKLLQEPDILSGDYDIHWLEKWAARQAAKA
                     "
     gene            2649..3314
                     /gene="aat"
                     /locus_tag="VF633_00090"
     CDS             2649..3314
                     /gene="aat"
                     /locus_tag="VF633_00090"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX8469487.1"
                     /translation="MSDVFGPAELLQCYATGVFPMGEARDDPRIFLVEPEERGVIPLD
                     GFHIPTRLKRTVRTEPFVVRVSTAFDAVLDACASPAPGREDTWINGPIRRLYLALQAM
                     GHAHSIECWKDERLVGGLYGVTLGGAFFGESMFSRERDASKVALVHLVARLKRGGWRL
                     LDAQFLTEHLTQFGAVETPQASYLDMLKAARSIRPNARSMFEPLSGEAAVFYALQPTT
                     QAS"
     gene            complement(3281..3937)
                     /locus_tag="VF633_00095"
     CDS             complement(3281..3937)
                     /locus_tag="VF633_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEX8469488.1"
                     /translation="MKRARLVGVTIAALALVGVGGVVVAGGLQEGPPQDARPSDDPIA
                     DLLRNQTPVEAAPAGVPAPNVPVAIVPSAPVDMTTPPQETPAETAEAKEDAKVPAEEK
                     IVEADTPLPRQRRRVAIVEAIDKITAQRMRFEVVVGGAPVRFNDSLTFSARACEVSAE
                     NELVSDAIAYLDISLQPKGMVQATEPRQIFRGWMFASTPGVSGLQHPIYDAWVVGCKA
                     "
     gene            complement(3937..>4298)
                     /locus_tag="VF633_00100"
     CDS             complement(3937..>4298)
                     /locus_tag="VF633_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEX8469489.1"
                     /translation="ATIGTLFTIAKRGELIGSDEMGNRYYQSRDNISYDGRKRRWVVY
                     EGYAEASKVSPDWYGWLHYTFAEPPTVEPLKRRAWELDHLPNLTGTPMAWRPPGSMAA
                     DGVRPAATGDYEAWKPE"
BASE COUNT          792 a         1428 c         1336 g          742 t
ORIGIN      
        1 tttcgccggg gcgctttgac ccgggccgtc agtcagttaa aaggccgccc gattcatcgg
       61 cgtcctccag ttagaaacgc cccggagcct aaccgcaggc aatgcccgaa acgccagtca
      121 tctacgtcct gaacggtccc aatctcaacc tcctcggggt tcgtgagccg gatatctatg
      181 gatatgagac gctcgcggac gttcaggctc tgtgcgagaa ggcggctgaa ggccacacgg
      241 tcgtgttccg ccagagcaac cacgaaggcg acctgatcga ctggatccag gaggctcgca
      301 ccgaggcttc ggcgctggtc atcaatccgg caggctatgg tcatacctcc gtggccctgc
      361 tggacgcgct aaagacgctg agcattccaa tcatcgaatg ccacctgtcc aatccggcga
      421 cgcgcgagcg tttccgacat aagacctatg tttcgctggt tgcgaccggc gtcgtcgcgg
      481 gcttcggctc gcaaagctac gaactggccg tcagggccgc agtgaagctc gttgcggaac
      541 gcgcagcagc cgccacccgt tcgtcccaag gataagaaga aggtttccac acgcatggcc
      601 gaccctaaat cgacccccgc caaactgaac aatgatgttg agatcgacgc ggcgctggtg
      661 cgccaactcg ccgacatcct caacgacacc tccctgaccg agatcgaggt cgaacgtggc
      721 gaactgcgca tccgcgtcgc acgcgagatc accgccgcgc cagtgatgca gtacgcagcc
      781 gcaccccagt ttgcggctca gcctcaggcc gcgcctgccg cagccgcccc cgtgtcaatg
      841 ccctccgacc cagccaccat cgtcgccaag gccggcgaga ccgtgaaatc gccgatggtc
      901 ggcaccgcct atctgcaggc ctcgcctgaa gccgcgccgt tcatcaagat cggcgacacg
      961 gtcaagaagg gccagacgct cgtcatcgtc gaggcgatga agaccatgaa cccaatccag
     1021 gccccgcgtg acggcgtggt cgccgaaatt ctcgtcggcg acgcccagcc ggtcgaatac
     1081 ggcgaacccc tcgtcctgct cgagaactga gcctgaccat gttcaccaag gtcctgatcg
     1141 ccaaccgcgg cgaaatcgcc ctgcgtattc accgggcgtg caaggagatg ggcatctcca
     1201 ccgtagccgt gcactccgag gcggatcgcg gcgccatgtg ggtgcggctg gctgacgaaa
     1261 gcgtctgcat cggccccgct ccggcggcga agtcgtatct gaacatcccg tcgatcatcg
     1321 cggcggccga gatcacgggc gcccaggcga tccacccggg ctatggcttc ctgtccgaaa
     1381 acgcgcgttt cgccgagatc gtcgaagccc acggcatgag cttcatcggt ccccagcccg
     1441 agcatatccg ggtcatgggc gacaagatca gcgccaagaa gacggtgctg gcagccggga
     1501 tcccttgcgt ccccggctct gacggcgaag tcgaaaccgt cgaggacgcg atcgaggcgt
     1561 cgaagtccat cggcttcccg ctgatcgtaa aggcggcggc cggcggcggc ggacgcggca
     1621 tgaaggtcgc cctaacggct gacgatctgg tcgaggcggt gcaaaccgcc cagaccgagg
     1681 ccaaagcggc gttcggcaac ggcgccgtct acatggagcg ctatctccag aagccgcgcc
     1741 acatcgagat ccaggtcatc gccgacagcc acggcaacgt cgtccatttg ggcgagcgcg
     1801 actgttcgct gcagcgtcgc caccagaagg tgctggaaga ggccccctcg cccgccctgt
     1861 cggcggaagg tcgcaaagct atcggtgaaa cggtcaacaa ggccatcgcc gccatcggct
     1921 acctcggcgt cggcacgatc gaattcctgt gggaagacgg cgagttcttc ttcatcgaga
     1981 tgaacacccg cctgcaggtc gaacacccgg tcaccgaggc catcaccggc gtcgatctgg
     2041 tgcgtgaaca gatccgcatc gccgcaggcc tgcccctgtc gttcacccag aacgacatcc
     2101 atttcgaagg ccacgccatc gaggttcgga tcaacgccga gaacgccgag accttcaccc
     2161 cgtcgcccgg caccatcacc gacttccacg ccccgggcgg tctgggcgtg cggctggata
     2221 gcgccatcta cgccggctat tcgattccgc cctattacga cagcctgatt ggcaagctga
     2281 tcgtgcatgg tcgggaccgg gcggaatgta tcgctcgcct caagcgcagc ctgaacgaga
     2341 tggtcatcgg cggcatcgac accaccattc cgctattcca gaaactgctt caggagcccg
     2401 atatcctgag cggcgactat gacatccact ggctcgagaa gtgggccgcg cgtcaggccg
     2461 cgaaagcctg aaagagtcac ctctccatcg ccgaagggcg gtggggagga cagaccgcaa
     2521 agcggtcagg agggggctac gccgccagct taaatgagct actcctccct tcccacaccg
     2581 tgtctccccc acctgatcgc tgcgcgatct gtcctcccca ttcgccttcg cgaatggaga
     2641 ggtgaacgat gagcgacgtc ttcggcccgg ccgaactcct ccaatgctat gcgaccggcg
     2701 tctttccgat gggagaggcg cgtgatgacc cgcgcatctt cctcgtcgaa ccggaggagc
     2761 gcggcgtcat tccgctggac ggtttccaca tcccgacgcg gttgaagcgg acggtgcgga
     2821 ccgagccgtt cgtcgtaagg gtttcgaccg cgttcgacgc agttctggac gcctgcgcct
     2881 cgcccgcgcc cggtcgcgaa gacacctgga tcaacggccc catacgtcgg ctctatctgg
     2941 cgctgcaggc catgggacac gcgcattcca tcgagtgctg gaaggacgag cggctagtcg
     3001 gcggacttta cggagtaacg ctgggcggcg cctttttcgg cgagagcatg ttcagccgcg
     3061 agcgcgatgc ctccaaggtc gcactggtgc atctggtggc gcggctgaag cggggtggct
     3121 ggcgattgct cgacgctcag ttcctgacgg aacatctgac ccagttcggc gcggtggaaa
     3181 cgcctcaagc gagctatctc gacatgctca aggctgcgcg gtcgatacga cccaacgctc
     3241 gttcaatgtt cgagcctctg tccggagagg cggcggtttt ctacgccttg cagcccacga
     3301 cccaagcgtc atagatcggg tgttgcaggc cgctgacgcc aggcgtcgag gcgaacatcc
     3361 agccgcggaa gatctggcgt ggctcggtcg cctgaaccat ccccttgggc tgcaggctga
     3421 tgtccagata ggcgatggcg tcagacacaa gctcattctc ggcggacact tcacaggcgc
     3481 gcgcgctgaa ggtcaggctg tcgttgaagc gcaccggagc gccgccgacg acgacctcaa
     3541 accgcatccg ttgggccgtg atcttgtcga tcgcctcgac gatggccacg cgacgacgtt
     3601 ggcgcggcaa gggtgtatca gcctcgacga tcttctcctc ggcggggacc ttggcgtctt
     3661 ctttcgcttc tgcggtttcg gcaggcgttt cctgcggcgg cgtggtcatg tcgacgggcg
     3721 cgcttgggac gatcgcaacc ggcacattcg gggccggcac gcctgctggg gcggcctcca
     3781 cgggcgtctg gttgcgcaga aggtcggcga ttggatcgtc ggaaggacgc gcgtcctgcg
     3841 gcgggccctc ctgcaggccg cctgccacga cgaccccgcc tactcccact aacgccagtg
     3901 cggcgattgt gacgccgacc agccgcgcac gtttcattat tccggtttcc aggcttcgta
     3961 gtcgccagta gcggcggggc gcacgccgtc ggcagccatc gagccgggcg gcctccacgc
     4021 cataggcgtg ccggtcaggt tcggcaggtg gtccagttcc caagcgcggc gcttcagcgg
     4081 ctcaactgtg ggtggctcgg caaaggtgta gtggagccaa ccataccaat cgggggagac
     4141 cttggacgcc tcggcataac cttcgtacac cacccagcgg cgtttgcggc cgtcgtagct
     4201 gatgttgtcg cggctctggt aataccggtt gcccatctcg tcagagccga tcaactcgcc
     4261 acgcttggcg atggtgaaaa gcgtgccaat ggtcgcgc
//
LOCUS       DASVTM010000004         7822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_851593,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000004 DASVTM010000000
VERSION     DASVTM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851593"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF633_00105"
     CDS             complement(<1..314)
                     /locus_tag="VF633_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8469490.1"
                     /translation="MAGDPYKELGVSRGASADEIKKAFRKLAKELHPDKNPGDKAAEE
                     RFKRLTGAFDVIGDAEKREKFDQGQIDADGREQYAGFGGPRGGATPGAGGGIGGFGQE
                     GGP"
     gene            complement(416..2155)
                     /locus_tag="VF633_00110"
     CDS             complement(416..2155)
                     /locus_tag="VF633_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX8469491.1"
                     /translation="MSRIERDWKSWRGPAQKAPRDDWTTWLLLGGRGAGKTRAGAEWL
                     TRRAVKGARLALIGPTLHDVREVMIEGPSGLKAVAAPGDRPTYQSSRRLLEWPSGARA
                     FAFSAEDPESLRGPQFHGAWADEFCAWRKPGEVLAMLRLGLRLGDDPRLMVTTTPRPL
                     PALKTLIAEPGTILTRAPTAANAINLSAGFLTGLNRLYGKTRLEAQEVEGLILDDAGG
                     LWSLAILNGCRRAAPERFDRVVVGVDPPATSHGDACGIVVAGRLGDDAYVLADLSVKN
                     ASPLKWAEAVSEAARRFRADRVIVETNQGGEMVGQMLKFAGCGSPVTPVFARVGVLTQ
                     ALAPGVTGRWDEANAVVVRVEGGAPASLGETVVLGGGNALAIQVVGGWEIVQFRTATL
                     VGDGVWRLTGLLRAQQGTDAEMAVGAAVGSLVVFLDDTLGRASVSSSEVGLPLIWRAG
                     PAGGPAGGPSVSERLVTLRGLHHRPWSPAHVRVRQNATGLGIDWIARSRLDGDIWDRE
                     VSADPMRFRVRVLDGAVVVRIWEVEGLGAAYATSAMASDFPGGFGPDMRIGVAQDSVI
                     FGWGSEAIVKVPA"
     gene            complement(2091..2426)
                     /locus_tag="VF633_00115"
     CDS             complement(2091..2426)
                     /locus_tag="VF633_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469492.1"
                     /translation="MLIQDLNRRIAEAAEGPLDGMERTAKAVGVIVKAVQSVIELGGG
                     DETGNLDEGLDAEAEQHLRDQFLGRLDAIAAALELEGVRRCPACGRFEPDRAGLEKLA
                     RSGAEGAEG"
     gene            complement(2604..3059)
                     /locus_tag="VF633_00120"
     CDS             complement(2604..3059)
                     /locus_tag="VF633_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEX8469493.1"
                     /translation="MKPFWQTKRLEQMTREEWESLCDGCGQCCLIRFEDEDSGQVFAT
                     SVHCRLFDSQKCTCIDYANRKAHVPDCIKLTPWNIDALQWMPKSCAYRRINEGRDLAD
                     WHPLISGDPESVHQAGVSVRGQTISELDLADPEDAVDFAAPEWDVERGR"
     gene            complement(3102..3440)
                     /locus_tag="VF633_00125"
     CDS             complement(3102..3440)
                     /locus_tag="VF633_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX8469494.1"
                     /translation="MSPLTWAALGGAIALEVVGTTLLQQSQQFTRLWPTVGMALCYGV
                     AFYLLSVALKQMPVGIAYAIWSGLGVVAISVIGLVLFRQKLDLPAIIGLTMIVGGVIV
                     INLFSKTTGH"
     gene            3684..5501
                     /locus_tag="VF633_00130"
     CDS             3684..5501
                     /locus_tag="VF633_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX8469495.1"
                     /translation="MAPRPPLVALNDVRLQDGARPLFDGVDLAIEPRTRSCLVGRNGA
                     GKSTLMKVVMGLIEPDSGTRSVQGAVKFAYVPQEPIIAGDTLLDYATSNDAESWTAEA
                     WLETFGMDPSKSTKGLSGGETRRAALAKAFAEEPDLLLLDEPTNHMDILAIELLEKEI
                     IAARCAALIVSHDRAFLNRVTQSCHWLEGRKVRTLNKGFDAFDEWSTKQMEEEALAFQ
                     KLTKTIERETATFYSSITARRSRNEGRARSLDALRAERSEQAKDRPREMQLGVDSGGT
                     SGKLVADLKGVTKSFGDRVVIKPFTSRIIRGDRLAIVGPNGAGKTTLVKIMLGELAPT
                     DGTVKLGASLETVYLDQSREGLKSDMTLWDALTPGGGDSIIVRGISKHVAAYAKDFLF
                     TDAQLRQPISTLSGGERNRLLLAIALAKPANMLVLDEPTNDLDMDTLDKLEELLEQYD
                     GTLILVSHDRDFVDRLATSTIGMNGRGDIVETPDGWSDFIRQNPGFLKPIAAGSSQAA
                     TKVEPAPVTPTPTVAKGKMSFKDSHRLKELDAMIHELPDTIKGHQKRLDDVNFYSRDP
                     AGFASTMKALEAAQAKLAAAEEEWLGLEEKREALTGSPA"
     gene            5546..6553
                     /locus_tag="VF633_00135"
     CDS             5546..6553
                     /locus_tag="VF633_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX8469496.1"
                     /translation="MALTVRTLPLTLAQTRAARRTNAVMAIAPRLKLGGWRLQLGVRL
                     NPIAARAAGRLSAASLKRRGVTVSSTSIGPATQPIPLRILQPSTPPRALIVDFHGGGW
                     VIGSAALNDPLTGHFAEAGFAVASVDYRLLDEARQIWVPDAVEDCVAALRWALDEGRT
                     QFGVDDVFLIGESAGAHLAALALLKLRDEGASRLPACIFVQGVFDLSGTPSVRAAGRE
                     ALLFDGPNLTRDLGLLTPGLDEAGRRHPDLSPLYADLGSLPPALFVMGEADPLRDDSI
                     LMAEAWGRHNTAALLDVPTAPHGFQHFGAPTAALVQSAIRDWISDKIVADGVSVENTN
                     R"
     gene            7124..>7822
                     /locus_tag="VF633_00140"
     CDS             7124..>7822
                     /locus_tag="VF633_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8469497.1"
                     /translation="MSRDQNRPPNASRRIVLAGGAIAAATAALTGLTSCFRSEAPVDD
                     QGRVRIRFATDWHAQAEHGGFYQALASGAYEKRGLNVQIIQGGPNVNMPQLLASGAVE
                     LGMGSNSFIPFNLVAENAPVKAVAAFFQKDPQVLIAHPDPDLKAIADLKDRPFLLADA
                     SITGFWVWLKTKYGFTDEQVRKYTFGPTPFLEDELAVQQGLLTSDPYVIEQAGDIEPK
                     VFLLADEGYPSYAAM"
BASE COUNT         1401 a         2890 c         2214 g         1317 t
ORIGIN      
        1 gggccgcctt cctggccaaa accgccaatg ccgccgccag cgccgggggt agcgccgccc
       61 cgaggtcccc cgaatccagc atattgctcg cgaccgtcgg cgtcgatttg cccttgatcg
      121 aatttctcgc gcttctcggc gtcgccgatc acatcgaacg cgcccgtcag ccgcttgaaa
      181 cgttcctcgg cggccttgtc gccgggattt ttgtcagggt gcaattcctt ggccagcttg
      241 cggaaggcct tcttgatctc atccgcgctc gcgcccctcg aaacgcctag ttccttgtag
      301 ggatcgcccg ccacgtctga tcatgctccg ccagaaagct gcccgacgca cctcgcgcct
      361 gaaccgccgt ttgaatgccg tcagttaagg catggctgcg tcaaagcaat ggctttcaag
      421 ccggaacctt gacgatggcc tcgctgcccc agccgaaaat cacgctgtcc tgagccacgc
      481 cgatccgcat atcgggccca aagccgcccg gaaagtcgct cgccatggcg ctcgtcgcat
      541 aggcggcgcc aagcccctcg acctcccaaa ttctaaccac gacagcgccg tcaagcaccc
      601 gaaccctgaa ccgcatcgga tcagcgctca cttcgcgatc ccagatgtct ccatccagtc
      661 tggaccgggc gatccaatcg atgcccagac ctgtcgcgtt ctgccgaacg cgaacatgcg
      721 cagggctcca cggcctgtga tgcaatccgc gcaatgtaac caaccgttcg gagaccgatg
      781 ggccaccggc tggaccaccc gctggtccag cccgccagat caggggcaac ccgacctcgc
      841 tcgacgaaac cgacgccctg cccaaggtgt cgtcgagaaa caccacgagg ctaccgacgg
      901 cggcccccac ggccatctcg gcatccgtgc cctgctgcgc ccgcaacagc ccggtcagcc
      961 tccagacgcc atcgcctacc aaggtcgcgg ttcgaaactg cacgatttcc cagccgccca
     1021 cgacctgaat cgcgagagca ttccctcccc ccagaaccac cgtctctcca aggctggcag
     1081 gggcacctcc ctcaacccgc accaccacgg cgttggcctc atcccagcga cccgtgacgc
     1141 cgggcgccaa cgcctgcgtc agcacgccca cccgcgcgaa caccggcgtc accggcgacc
     1201 cgcaccctgc gaacttcagc atctggccga ccatctcgcc gccctgattg gtctcgacga
     1261 ttacccggtc ggcccggaac cttcgtgcgg cctccgacac cgcctcggcc catttcagcg
     1321 gcgaggcgtt cttcacgctc aggtccgcca gcacataggc gtcgtcgccc agtctccccg
     1381 ccactacgat cccacaggcg tcgccatgtg aggtcgctgg cggatcgaca ccgacgacga
     1441 ccctgtcgaa acgttcaggc gccgccctcc ggcacccgtt caaaatcgcc agcgaccaca
     1501 gcccgccggc gtcatcgagg atcagcccct cgacctcctg cgcctccagc cgggtcttgc
     1561 cgtaaagacg attgagcccg gtgaggaaac ccgctgacag gttgatcgca ttggccgccg
     1621 tcggcgcccg cgtcaggatc gtccccggct ctgcgatcag cgtcttcaac gcaggcagag
     1681 gtctcggcgt cgtcgtcacc atcagccggg gatcgtctcc cagcctcagc cccagccgca
     1741 acatcgccag aacctccccc ggctttcgcc aggcgcagaa ctcatccgcc caggccccgt
     1801 gaaactgggg cccgcgcaaa ctctccggat cctccgccga aaaggcgaaa gcccgggccc
     1861 cgctgggcca ttccagcaac cggcggctgg actgataggt gggtcgatcg cccggcgccg
     1921 ccacggcctt cagccccgac ggcccctcga tcatcacctc acgcacatcg tgcaaggtcg
     1981 gtccgatcag ggccagccgc gcgcccttca cggccctccg cgtcagccac tcggcgcccg
     2041 cccgcgtctt gccggctccg cgcccgccca gaagcagcca cgtcgtccag tcatccctcg
     2101 gcgccttctg cgccggaccg cgccagcttt tccagtcccg ctcgatccgg ctcaaatcgg
     2161 ccgcacgcgg gacatcggcg tacgccttcc agctcgagcg cggctgcaat agcatccaga
     2221 cggcccagga attggtctcg aagatgctgc tcggcttcgg cgtcgagtcc ttcatccaaa
     2281 tttcctgtct cgtcgccgcc accgagctcg attacggact ggaccgcctt gacgatcacg
     2341 cccacggcct tggccgtgcg ttccatcccg tcaagcggcc cctccgccgc ctcggctatt
     2401 cgtcggttca ggtcctgaat cagcatcgcg acatccagcg tgtcccgccc cgccgcgtcc
     2461 ctttttccgt ccatgcccag actatagagg accagcgacc tcaggcgggt tcagcaggtc
     2521 gagaaaagcg caaacccggg cccgctctgg cattggcgcc gccaatcccg ggcgttcacc
     2581 ggatcgggcg taggtatggc ggcctatcgg cctcgctcga cgtcccattc gggcgctgcg
     2641 aaatccacag catcctcagg atcggccagg tcgagctccg agatcgtctg accccgcact
     2701 gaaacgccgg cctgatgcac gctctcggga tcgccggaga tcagcggatg ccagtccgcc
     2761 agatcacgtc cctcgttgat ccggcgatag gcgcacgact tcggcatcca ctgcagcgca
     2821 tcgatattcc acggcgtcag cttgatgcaa tccggcacat gcgccttacg gttcgcatag
     2881 tcgatgcagg tgcatttctg gctgtcgaac aggcggcagt ggacgctcgt cgcgaacacc
     2941 tggcctgaat cctcgtcctc gaaccggatc aggcaacatt gtccgcaccc gtcacacagg
     3001 ctttcccact cctcgcgggt catctgttcc aggcgcttgg tttgccaaaa gggcttcatg
     3061 gcgcgcctct tacgccgcga tccgggcgat gtcgcgaccg atcagtgccc tgtcgtcttc
     3121 gagaacaggt tgatgacgat caccccgccg acgatcatcg tcagaccgat gatggccggc
     3181 aagtccagct tctggcgaaa cagcaccagc ccgatcaccg agatcgccac caccccaagc
     3241 ccgctccaga tcgcataggc gataccgacc ggcatctgct tcagggcgac cgacaacaga
     3301 tagaacgcca cgccatagca aagcgccatt cccaccgtcg gccacagtcg ggtgaactgc
     3361 tggctctgct gcagcagtgt cgtccccacc acttccagcg cgatcgcccc gcccagcgcc
     3421 gcccacgtca gcgggctcac gccgcagtct ttttgtagcc ggtggcctgc tcggccgttc
     3481 ggtccggaat acagccaatc gtccgcacgt catatgcgac ctctgaggct ttttcgatga
     3541 taacagcacg cgtctcgggt gcgcgaagac ggccttggat actcattagc ggctcatacc
     3601 gtccgggcgg tacaaatcaa aatgtcttcg cagtcgcagc atgtggatgg caacaggccg
     3661 tcgctcccta tatacggccg gccatggctc ccagaccccc cctagtcgcc ctcaatgacg
     3721 ttcgtctgca ggacggagcg cgtcctctgt tcgatggcgt cgaccttgcg atcgaacccc
     3781 gcacccgctc ttgcctcgtc ggccgcaacg gcgccggcaa gtccaccctg atgaaggtcg
     3841 tgatgggcct gatcgagcct gactcgggca cgcgctccgt ccagggcgcg gtcaagttcg
     3901 cctatgtgcc tcaagagccg atcatcgccg gcgacaccct gctggactac gccacatcca
     3961 acgacgccga gtcctggaca gccgaggcct ggctcgagac cttcggcatg gacccgagca
     4021 aatccaccaa gggtttatcc ggtggtgaga cccgtcgcgc cgcactcgcc aaggcttttg
     4081 ccgaggagcc cgacctgctc ctgctagacg agcccaccaa ccacatggac attctggcga
     4141 tcgagctgct ggaaaaagag atcatcgccg cgcgctgcgc cgccctgatc gtctcccacg
     4201 atcgcgcctt cctgaaccgc gtcacccagt cctgccactg gctggaaggc cgcaaggtcc
     4261 gcaccctgaa caagggtttc gacgctttcg acgaatggtc caccaagcag atggaagaag
     4321 aggctctggc cttccagaag ctgaccaaaa ccatcgagcg cgaaaccgcg accttctaca
     4381 gctcgatcac cgctcgccgc agccgtaacg aaggccgcgc acgctctctg gacgccctgc
     4441 gcgccgagcg ttccgaacag gccaaggatc gcccgcgcga aatgcagctc ggcgtcgatt
     4501 ccggcggcac ttcgggcaag ctggtggccg acctcaaggg cgtcaccaaa tccttcggcg
     4561 accgtgtcgt catcaagccc ttcaccagcc gcatcatccg tggcgaccgg ctcgccatcg
     4621 tcggcccgaa cggcgccggc aagaccacgc tggtcaagat catgctcggc gagctggctc
     4681 caaccgacgg cacggtcaaa ctcggcgcca gcctcgagac ggtctacctc gaccagtccc
     4741 gtgaaggcct gaagtccgac atgaccctgt gggacgccct gacgcccggc ggcggcgact
     4801 ccatcatcgt gcgcggcatc tccaagcacg tcgccgccta cgccaaggac ttcctgttca
     4861 ccgacgccca gctgcgccag ccgatctcga cgctgtcggg cggcgagcgt aaccgcctgc
     4921 tgctggccat cgcgctcgcc aagcccgcca acatgctggt gctcgatgag ccgaccaacg
     4981 acctcgacat ggacacgctc gacaagctcg aagagctgct cgagcaatat gacggcaccc
     5041 tgatcctggt cagccacgac cgcgacttcg tcgaccgcct cgccacctcg accattggca
     5101 tgaacggtcg cggcgacatc gtcgagaccc ccgacggctg gagtgatttc atccgtcaga
     5161 accccggctt cctcaaaccc atcgccgccg gcagcagcca ggccgccacc aaggtagagc
     5221 ctgcccccgt caccccgacg cccacggtcg ccaagggcaa gatgtccttc aaggactcgc
     5281 accggctcaa ggagctggac gccatgatcc acgagctgcc ggacaccata aagggacacc
     5341 agaagcgact cgacgacgtc aacttctact cccgagaccc tgccggtttc gcctccacca
     5401 tgaaggccct cgaagccgca caggccaaac tcgccgctgc ggaagaagaa tggctcggac
     5461 tggaagagaa gcgtgaggcg ctcacaggct cacccgcctg acgtgaccgg cctgacgtga
     5521 ccaacccgac gcgctagact ccgccatggc cctgaccgtt cgcaccctgc ctctgacgct
     5581 cgcgcaaacc cgcgcggccc ggcggacgaa cgcggtcatg gcgatcgccc ctcggctgaa
     5641 gctcggaggc tggaggctac agctcggtgt gcgcctgaac ccgatcgccg cgcgcgccgc
     5701 aggccgtctc agcgccgcct ccctcaagcg ccggggcgtc actgtctcct cgacgagcat
     5761 cggccctgcg acacagccga tcccgctccg cattcttcaa ccctcgaccc ctcctcgcgc
     5821 cctcatcgtc gacttccacg gcggcgggtg ggtcatcggc agcgccgcgc tcaacgatcc
     5881 cctgaccggt catttcgccg aagccggttt tgcggtcgcc tccgtcgatt atcgcttgct
     5941 ggacgaggcc cgccagatct gggtgcccga tgctgtcgag gattgcgtcg ccgccctgcg
     6001 ctgggctctg gacgagggcc ggacgcagtt tggcgtcgac gatgtctttc tgatcggaga
     6061 gtccgcgggc gcccacctgg ccgcgctcgc ccttctgaaa ctgcgcgacg aaggcgcaag
     6121 ccgcctgccg gcctgcatct tcgttcaggg cgtgttcgac ctgtccggaa ccccctcggt
     6181 ccgagccgct ggtcgtgaag cccttctgtt cgacggcccc aacctgaccc gcgaccttgg
     6241 ccttctgacg ccggggctgg acgaggcggg ccgtcgtcac cccgacctgt ccccgctcta
     6301 cgccgatctt ggcagtctgc cgcctgccct gttcgtgatg ggtgaggccg accctttgcg
     6361 cgacgacagc atcctcatgg ccgaagcctg gggacgccac aacacggcgg ccctgcttga
     6421 cgtgccgacc gccccgcatg gcttccagca tttcggcgct cccaccgctg cattggtgca
     6481 aagcgcgatc cgggactgga tatccgacaa gatcgtcgcc gatggtgttt ctgtggaaaa
     6541 cacaaaccgc tgatactccg gatggatttc gatccgttca cagaagttaa caggcttgtc
     6601 caccagcggg cgcgaacatc tgcacaggct cccaatctga gcgcttgtcg tcgacccgtt
     6661 tcagagcgag cttgatcagg ccgagggaca cggcagcgcg gaaatccggt gagaaccggg
     6721 acttcaaagc gggtcggtcc ggctttctga ccagacggtc ttccatcggc gctctcgggc
     6781 gacgaagcga gacaggacgg caggacacag gctctgacag acggaaccgc ttcggcggga
     6841 aagtctgaaa agggaaccgg gtcgggatcg aggcacgacg cgtggtttag agaccccgcg
     6901 acacgacttc ggacaggagc cgacaggata cagcggacca tctccggggc ttgcctcgca
     6961 ggtgatgacg ccagggccct gaacctcaat ggccaagtcc aacccggact tggtgagggg
     7021 acgaaagcga cggttcgccg gcgcgttcgt cccctcgctc aattctgcac cggagcgccc
     7081 gcgcgccttt ccttcgccgc gcccgccctc tagcctgcgc gccatgtcgc gggaccagaa
     7141 tcgcccccca aacgccagcc gccgtatcgt ccttgcgggt ggtgcgattg cggctgcaac
     7201 cgctgcgctg accggtctca catcctgctt ccgctccgag gcccccgtcg acgatcaggg
     7261 ccgggtgcgc atccgcttcg cgaccgattg gcacgcccag gccgaacatg gcggcttcta
     7321 tcaggcgcta gcgtccgggg cttatgagaa gcgcggcctg aacgtccaga tcatccaggg
     7381 cggtcccaac gttaacatgc cccaacttct ggcttccggt gcggtcgagt tgggcatggg
     7441 ctccaactcc ttcattccct tcaatttggt cgccgagaac gctccggtga aggccgtcgc
     7501 cgccttcttc cagaaggatc cgcaggtcct gatcgcccac cccgatcccg acctgaaagc
     7561 catcgcagac ctgaaggacc gcccgttcct gctggccgac gcctcgatca ccgggttctg
     7621 ggtctggctg aagaccaagt atggcttcac cgatgagcag gtccgcaaat acaccttcgg
     7681 tcccacgccc ttccttgagg acgaactggc ggtgcagcag ggcctcctaa cctctgatcc
     7741 ctatgtgatc gaacaggccg gcgacatcga gccgaaggtc ttcctgcttg ccgatgaggg
     7801 ctacccctcc tacgcagcca tg
//
LOCUS       DASVTM010000005         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_160392,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000005 DASVTM010000000
VERSION     DASVTM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160392"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF633_00145"
     CDS             complement(<1..382)
                     /locus_tag="VF633_00145"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX8469498.1"
                     /translation="MRVGLFGGSFNPAHDGHAHVAETALSRLGLDRVVWLVSPQNPLK
                     GRQETAPLEGRIASARTFAKGPGMIVSDFETRIGTAWTIDTLRALKARHPGVHFVWLM
                     GSDNLASFHRWRGWTDIMRMMPVAV"
     gene            complement(541..720)
                     /locus_tag="VF633_00150"
     CDS             complement(541..720)
                     /locus_tag="VF633_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469499.1"
                     /translation="MSDKQTDGRTANGATPGGKPDALTSTKDVKENGRREAKTAGPDG
                     PDGGEIGKTFKKTGG"
     gene            complement(783..1835)
                     /gene="obgE"
                     /locus_tag="VF633_00155"
     CDS             complement(783..1835)
                     /gene="obgE"
                     /locus_tag="VF633_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX8469500.1"
                     /translation="MKFLDQAKIYIRSGNGGAGSVSFRREKFIPNGGPDGGDGGNGGF
                     VWIEAVEGLNTLIDYRYKQHFKADTGNHGQGRQMHGAKGEDVVLKVPVGTQVLGEDKE
                     TVVLDMTEVGQMECLLRGGNGGWGNVRFKGPQNQAPRHANPGQEGQEMWIWLRLKLIA
                     DIGLAGMPNAGKSTFLAAASAARPKIADYPFTTLAPNLGMVDLSPSERFVIADIPGLI
                     EGASEGAGLGVRFLGHVERSASLIHLIDGTQDNVAEAYRIIRGELDAYGEGLADKAEI
                     LALNKIDALTPEVREQKAAELEAVAGRRPMLVSGVSGEGVPELLRAAWAEVRRNRGEI
                     DEDGDERVQLTEAWKP"
     gene            1923..>2130
                     /locus_tag="VF633_00160"
     CDS             1923..>2130
                     /locus_tag="VF633_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469501.1"
                     /translation="MTSSLITGLTPSLTSAVPAARDPQQARHEARVREAKAALETMRQ
                     QQTSATDQRKAAAKKKVDDLKARIQ"
BASE COUNT          355 a          729 c          679 g          367 t
ORIGIN      
        1 tcaccgccac aggcatcatc cgcatgatgt cggtccatcc cctccagcga tggaagctgg
       61 ccagattgtc tgagcccatc agccagacga aatgcacacc cggatgccgc gccttcagcg
      121 ccctcaaggt atcgatggtc caggccgtgc cgatgcgggt ctcgaaatcc gagacgatca
      181 tacccggtcc cttggcgaag gtccgggccg aggcgatgcg gccttcgagc ggcgcagttt
      241 cttgacgccc cttcagcgga ttttgcggcg agacaagcca gacgacccgg tccagaccca
      301 ggcggctcaa cgccgtctcg gcgacgtggg cgtggccatc gtgggcggga ttgaaggagc
      361 cgccgaacag tccgacccgc ataccgggcg acaggctcag cccgtccctg agcgccccgt
      421 gacggggacc cagcgtcttt ggcgcaggac cggcgtggaa gaaggacaag gcggacggcg
      481 ctctgacagg cttatggtca ggggtgcgct aacacgccgc cacgccggtg tcgcgaaaga
      541 tcagccgccg gtcttcttga acgtcttgcc gatctcgccg ccgtcgggac cgtccggacc
      601 cgccgttttc gcctcgcggc gcccgttttc cttgacgtcc ttcgtcgagg tcaacgcatc
      661 gggtttaccg cccggcgtcg ccccgttcgc agtgcgtccg tctgtctgtt tgtcgctcat
      721 gaccgctctc ctgtttcgcc tcagcgagcg ggagggtcac gggttccgtc agacgcgtgc
      781 gatcaaggct tccaggcttc ggtcagttga acgcgctcgt cgccatcctc gtcgatctcg
      841 ccgcggttcc ggcggacctc ggcccaggct gcgcgcagca gctcgggcac gccctcaccg
      901 gacacgccgg agaccagcat gggccggcgg ccggcgacgg cctccagctc ggcggccttt
      961 tgttcgcgga cctccggcgt gagggcgtcg atcttgttca gcgcgaggat ctcggccttg
     1021 tcggcgaggc cctcgccata ggcgtccagc tcgccgcgga tgatccggta ggcctcggcg
     1081 acgttgtcct gcgtgccgtc gatcaggtgg atcaacgagg ccgaacgctc gacgtgaccg
     1141 aggaaccgca cgccgagacc agcgccttcc gaggcgccct cgatcagtcc ggggatgtcg
     1201 gctataacga accgctcact gggcgacagg tcgaccatgc cgaggttggg cgccagcgtc
     1261 gtgaacggat agtcggcgat cttgggccgc gcagccgagg cagcggcgag gaaggtcgac
     1321 ttgccggcgt tgggcatgcc cgccagaccg atgtcggcga tcagcttcag ccgcagccat
     1381 atccacatct cctggccttc ctgacccgga ttggcgtggc ggggagcctg attttgcggt
     1441 cccttgaagc ggacgttgcc ccagccgccg ttaccgccgc gcagcaggca ttccatctga
     1501 ccgacttcgg tcatgtcgag aacaaccgtt tccttgtcct cgcccaacac ctgggtgccg
     1561 acgggaacct tgagcacgac gtcctcgccc ttggcgccgt gcatctgacg gccttggccg
     1621 tgattgccgg tgtcggcttt gaaatgctgc ttgtagcgat agtcgatcag ggtgttcaaa
     1681 ccctcgacag cctcgatcca gacgaagccg ccgttgccgc cgtcgccgcc gtcggggccg
     1741 ccgttgggga tgaatttctc gcgccggaac gagacggagc ccgccccgcc gtttccggaa
     1801 cgaatataga tcttggcctg atcgagaaat ttcatcgcgc gcctatagtc caacagagcg
     1861 gcaatttaca ggcaaagcgg cggtttacgt ctcgttaggc caatcacgac acggtcccgc
     1921 ccatgacgag cagcctgatc accggtctga cccccagcct gacctcggcc gtcccagcag
     1981 cgcgcgatcc ccaacaggcc cggcacgagg cccgggtcag ggaggccaag gccgctctgg
     2041 agaccatgcg gcagcagcag acctccgcga ccgaccagcg caaggccgcc gccaagaaga
     2101 aggtcgacga cctcaaggcg cgcatccaga
//
LOCUS       DASVTM010000006         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_798344,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000006 DASVTM010000000
VERSION     DASVTM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798344"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF633_00165"
     CDS             <1..629
                     /locus_tag="VF633_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8469502.1"
                     /translation="SGEGAFVDVSMLDSTIATMGWVVSNYLIVGQEPMPIGNDNFTAA
                     PSGAFKTAEGLLNIAANKQEQFVALCGLINREDLLADERFAKRDARKRNRAALTVEVE
                     AGLAARSAAEWEPVFNAAGVPAGRVLTVPQALDLPQTKHRGLIHTFDHVEALDRPLSV
                     ALGGYRMRDRDITLTSPPPTLGQHTDTILDQLGYSAGEIAALHEAAVV"
     gene            626..1507
                     /locus_tag="VF633_00170"
     CDS             626..1507
                     /locus_tag="VF633_00170"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX8469503.1"
                     /translation="MTGKTPEEQVQAASDWWRTDIIQMRPGVIEFRGYPVQDLIGRLS
                     FPDMMWLLLRGDVPTEGQGKLLEAALVASVDHGPHAPSIAIARMAVTCGVDLNTAMAS
                     ASNVLGDSHGGAGQQCMELYAMVREELAGGLEMDAAVDAALDRYNAVYGKIIAGFGHR
                     FHPVDPRAKPLLALVEAAAAEGIVTGEYVPIGWAVEAGIKRRTGKSIPMNIDGATAVI
                     FAELGFAPELGRGLFILSRSVGILAHAWEQKQQGRRNMGPMPKEIPFTYSGPEGRRLD
                     DVRPSLAATPTKSEDTP"
     gene            1504..3261
                     /locus_tag="VF633_00175"
     CDS             1504..3261
                     /locus_tag="VF633_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8469504.1"
                     /translation="MKALISHQLVRFLEERGVEHLFGLCGHTNIAVLTALEGSSVKFV
                     NVRHEQIAAHMADGYARAGRKTSVLLSHLGPGLTNAATGVANAALDSIPMVVIAGDVP
                     SHYYGKHPHQEVNLHADASQSEIYRPFCKRVWRVERPDLFPEILAKAFQLAETGRPGP
                     VLISVPMDIFSMEIDTELFDKRKPHTRTVIKPTLDEETAERMVVAMAEATRPVLYVGG
                     GVLISQASEELAAFADHLSIPVAHSLMGKGALPDDHPMTLGMTGFWGTKFINESCRNA
                     DYVMGLGTSFKEADCSSWEPDYTFNIPTSKLLQIDIDPNEIGRNYPVEIGAVADLKSA
                     LQVINRVARRLYPEGRKDDAVRAAIIDNRKTFKASNAEAQASDQFPMRPERILADARA
                     VLPRDAIITTDVGWNKNGVGQQFDIYTPGSILTPGGFATMGFGAPAALGAKLACPDRV
                     VVSLVGDGGFGQNPSMLATAYEENIAVIWIVMNNGAYGTIAGLQKAHFGTTIGTIFEK
                     DGKPYSTDYSVIARAYGVDAVRINAADEFKPALEAAIASGRPTVLDVVMENAPVPTNG
                     HWNIMDIYSPGKKVHHVAT"
     gene            3262..>3666
                     /locus_tag="VF633_00180"
     CDS             3262..>3666
                     /locus_tag="VF633_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8469505.1"
                     /translation="MTAAGSTPGGLPIAARLAALVVAGALFMEMLDATIIVTALPALA
                     RSFAVRPSDLGLGVTGYAVALAAFIPASGWAADRFGGRRMFIAAIALFTVASLICAAA
                     TTRPAFVGARILQGAAAAMMSPVGRLLILSSVE"
BASE COUNT          643 a         1259 c         1138 g          626 t
ORIGIN      
        1 agtcaggcga aggcgctttc gtcgacgtgt cgatgctgga ttcgacgatc gccaccatgg
       61 gctgggtcgt gtccaactat ctgatcgtag gtcaggagcc gatgccgatc ggcaacgaca
      121 acttcaccgc tgccccctcg ggtgccttca agaccgctga gggcctgctg aacatcgcgg
      181 ccaacaaaca ggagcagttc gtcgccctgt gcggcctgat caatcgcgaa gacctgctgg
      241 ccgacgagcg gttcgccaaa cgtgatgcgc gcaaacgtaa tcgcgcggcc ctgacggtcg
      301 aagtcgaggc cggactcgcc gcgcgatccg ctgcagagtg ggagcccgtg ttcaatgccg
      361 caggcgttcc ggctggccgg gtgctgaccg tgccgcaagc gctcgatctg ccacagacga
      421 agcatcgcgg cctgatccat acgttcgacc acgttgaggc cctggaccga ccactgtcgg
      481 tggcccttgg cggctaccgg atgcgtgacc gcgacatcac cctgaccagc ccgcccccga
      541 cgctgggtca gcacaccgac accattctgg atcaactcgg ctattcggcc ggtgagatcg
      601 cagccctgca cgaggccgcc gtcgtatgac cggaaaaact cccgaagagc aggttcaagc
      661 agcgtctgat tggtggcgga ccgacatcat ccagatgcgg cccggcgtca tcgagttccg
      721 gggttaccca gttcaggacc tgatcggccg cctgtccttc ccggacatga tgtggctgct
      781 gctgcgcggc gacgtaccga ccgagggtca gggcaaactg ctggaggccg cactggtggc
      841 gtccgttgat cacggcccgc acgcgccctc catcgcaatc gcccgtatgg ccgtgacctg
      901 tggcgtcgac ctgaacaccg ccatggcctc ggcctccaac gtgctggggg actcgcacgg
      961 tggagccggt cagcagtgca tggaactgta cgccatggtc cgcgaggaac ttgcgggcgg
     1021 gctggagatg gacgccgccg tagacgcggc gctcgatcgc tacaacgccg tctacggcaa
     1081 gatcatcgcc ggctttggac accgcttcca cccggtcgac ccccgcgcca aaccgctgct
     1141 cgctctcgtc gaggccgcag cagccgaggg catcgtcacc ggcgaatatg tgccgatcgg
     1201 ctgggccgtc gaggcgggca tcaagcgccg gacgggcaag tcgatcccga tgaacatcga
     1261 cggcgcgacc gccgtgatct tcgccgaact cggcttcgcg ccagagctcg gtcgcggcct
     1321 gttcatcctg tcccgctcgg tcggcatcct cgcccacgct tgggaacaga agcagcaggg
     1381 ccgtcggaac atgggtccga tgcccaagga aattcccttc acctattccg gccccgaagg
     1441 gcgacgtctc gacgatgtcc gcccttccct ggccgccacc ccgacaaagt cagaggacac
     1501 gccatgaagg ctctcatttc acatcagctg gtccgctttc ttgaggaacg cggcgtcgag
     1561 catctgttcg gcctgtgcgg ccacaccaac atcgccgtgc tgacggcgct cgaaggcagc
     1621 agcgtcaaat tcgtcaatgt gcggcacgag cagatcgcgg cccacatggc ggacggctat
     1681 gcccgtgcgg gtcgcaagac atctgtcctg ctcagccacc tcggtccggg cctgaccaat
     1741 gccgccaccg gtgtcgcgaa tgctgcgctc gactctatcc cgatggttgt catcgccggc
     1801 gacgtgccga gccattatta cggcaagcac ccgcaccagg aggtgaacct ccacgccgac
     1861 gcctcccagt cggagatcta ccgcccattc tgcaagcgcg tctggcgggt cgagcgtccg
     1921 gacctgttcc ccgaaatcct cgccaaggcg ttccagttgg ctgagaccgg gcgaccggga
     1981 cccgtgttga tctcggtgcc gatggacatc ttctcgatgg agatcgacac cgagctgttc
     2041 gacaagcgca agccccacac ccgcaccgtg attaagccca cgctggacga ggaaaccgcc
     2101 gaacggatgg ttgtggccat ggccgaggcg acccggcctg tcctgtacgt ggggggcggc
     2161 gtcctgattt cccaagcctc ggaggaactc gccgcgttcg ccgatcactt gagcataccc
     2221 gtcgcccact cgctgatggg caagggcgcc ctgcccgatg accatccaat gacgttgggg
     2281 atgacaggct tctggggcac caagttcatc aacgagagct gccgcaacgc cgactacgtc
     2341 atgggcctcg gcacctcgtt caaggaagcc gactgcagtt cctgggagcc ggattacacc
     2401 ttcaacatcc cgacctcgaa actgctccag atcgacattg accccaacga aatcggtcgc
     2461 aactacccgg tcgagatcgg cgcggtcgcg gacctcaagt cggccctgca agtgatcaat
     2521 cgcgttgccc gtcgcctcta tcctgaaggc cgcaaggacg atgccgtgcg agccgcgatc
     2581 atcgataacc gcaagacctt caaggcctcc aatgctgagg ctcaggccag cgaccagttc
     2641 cccatgcgcc ccgaacgcat cctcgccgat gctcgcgccg tcctgccgcg tgacgcgatc
     2701 atcaccaccg acgtcggctg gaacaagaac ggcgtcggcc agcagttcga catctacacc
     2761 cccggctcga tcctgacccc gggtggcttc gcgaccatgg gcttcggcgc ccccgcagcg
     2821 ctgggcgcca agctggcctg tcctgatcgg gtggtcgtgt cgctggtcgg cgacggcggt
     2881 ttcggccaga acccctcgat gctggcgact gcctatgagg agaacatcgc ggtcatctgg
     2941 atcgtcatga acaacggcgc ctacggcacc atcgcgggcc tgcagaaggc ccacttcggc
     3001 acgaccatcg gcaccatctt cgagaaggac ggcaagccct actcgaccga ttattcggtg
     3061 atcgcccggg cgtacggcgt cgatgccgtt cgcatcaatg cggcggacga gttcaagccg
     3121 gcgctggagg ccgctatcgc atcgggtcgc ccgacggtcc ttgacgtggt catggaaaac
     3181 gcgccggttc cgaccaatgg ccactggaac atcatggaca tctactcgcc cggcaagaag
     3241 gtccatcacg ttgcgacctg aatgacagcc gccgggagta cacccggcgg cctgccaata
     3301 gcggcgcggc tggccgccct cgtggtggcc ggcgcgctgt tcatggagat gctggacgcc
     3361 acgatcatcg tgacggccct acccgctctg gcacgctcgt tcgcggttcg gccgagcgac
     3421 ctcgggctcg gcgtcaccgg atatgccgtg gcgctggcgg cgttcattcc cgctagcggc
     3481 tgggctgccg accggttcgg cggacggcgg atgttcatcg ccgccatcgc cctgttcacg
     3541 gtcgcctcgc tgatctgcgc ggcagccacc acccggcccg ccttcgtcgg cgcccgtatt
     3601 ctgcagggcg cggcggccgc gatgatgtcg ccggtcgggc ggctgctgat cctgtcgagc
     3661 gtcgag
//
LOCUS       DASVTM010000007         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_693102,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000007 DASVTM010000000
VERSION     DASVTM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693102"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VF633_00185"
     CDS             complement(<1..624)
                     /locus_tag="VF633_00185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8469506.1"
                     /translation="MSKAKSSKVCVVAAGGTGGHMFPAEALSREMAARGWRVVLATDH
                     RGEQYAANFPAEEKLYLDTATGSGPIALLKAGVAITRGVLQAQAAFKRLDAGVVVGFG
                     GYPSAPALVAALTQGLPTVIHEQNAVLGRTNRLLASRVKAVASSFPTLARAPAGLNAR
                     VVGSPVRAEIRALFDRAYVAPVEGGPIQVLVTGGSQGARILSETTPRS"
     gene            complement(632..1816)
                     /gene="ftsW"
                     /locus_tag="VF633_00190"
     CDS             complement(632..1816)
                     /gene="ftsW"
                     /locus_tag="VF633_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX8469507.1"
                     /translation="MTSPSYSPAFSRNDQSPIAQWFWTVDRGLLGAALILMSMGVALS
                     FASSPAAILADESITDPFHYAWRMIVWALGGVGLMLTVSLFSPKGVRRLAVIGLLGAI
                     VVMAALPFIGDEVKGAARWINLGPFSLQPSEFVKPCLIVFASWMFAEGQKGQGVPGVS
                     IAFGFWALVVGLLLIQPDIGQTLLITTTFMAVFFMAGVPLKWVAVLAAAACGGVVSLY
                     FMFGHMRDRLSRFTNPETTDTHQIDRASDAIRAGGLFGRGVGEGVMKRHVPDLHTDFI
                     YSVAAEEFGLVLSLTIIGLYAFIVIRGMRRAMKLNDPFEQTAAAGLFILIGLQACINV
                     AVNLNLIPTKGMTLPFISYGGSSMLAMGLTMGLALALTRRRPGAYEPGASLSRPRRRI
                     LP"
     gene            complement(1823..>2150)
                     /gene="murD"
                     /locus_tag="VF633_00195"
     CDS             complement(1823..>2150)
                     /gene="murD"
                     /locus_tag="VF633_00195"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270186.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX8469508.1"
                     /translation="FWIAGGKAKDGGIDDLTDLFGRVTKAYLIGEAAEAFAATLGDTP
                     HIISKTMEAAVAAAAEDAAAAGGEQIVLLSPACASFDQYPDFEARGEAFRAAVLNLGA
                     TPSVHG"
BASE COUNT          399 a          740 c          693 g          318 t
ORIGIN      
        1 cgacctgggc gtggtttccg acagaatgcg cgcaccctga ctgccgccgg tcaccagcac
       61 ctgtatcggc ccgccctcga ccggagcgac gtaggcccgg tcgaacagcg cgcggatttc
      121 agcccgcacc ggactgccga cgacccgagc attcagccct gccggagccc gcgccagcgt
      181 cggaaacgac gaagccacgg ctttcactcg cgaggccagc agacggttgg tgcggcccag
      241 cacggcattc tgttcgtgaa tcaccgtcgg cagtccctgc gtcaacgccg ccacgagcgc
      301 cggggccgag ggatacccgc caaacccgac gaccacgccg gcgtccagtc gtttgaacgc
      361 cgcctgcgcc tgcagaacac ctctggtgat tgccacgccc gctttcagca gagcgatggg
      421 tccagagccg gtcgcggtgt cgagatagag tttttcctcg gccgggaagt tcgccgcata
      481 ctgctcgccg cgatgatccg tcgccagcac cacgcgccag cctcgcgccg ccatctcgcg
      541 tgacaacgcc tcggccggga acatatgtcc gccggtgccc ccggcggcga cgacgcagac
      601 cttggaggat ttggccttgg acatggaacc tctaaggcag gatgcgccga cgcggacgcg
      661 acaggctggc gcccggctcg taggcgcccg gacggcgacg ggtcaaggcc agcgccagac
      721 ccatggtcaa ccccatggcc agcatcgacg agccgccata gctgatgaag ggcagcgtca
      781 tccccttggt cgggatcagg ttcaggttca ccgccacgtt gatgcaggcc tgcagcccga
      841 tcaggatgaa caaccccgcc gccgccgtct gctcgaacgg atcgttcagc ttcatcgccc
      901 ggcgcatgcc ccgaatgacg atgaaggcgt acaggccgat gatcgtcagg ctcagcacca
      961 gaccgaactc ttccgccgcg accgaataga tgaagtcggt gtgcaggtct ggcacgtggc
     1021 gtttcatcac gccctcgccg acgccgcgcc cgaacagccc gccggcgcgg atggcgtcgg
     1081 atgcgcggtc gatctggtgg gtgtcggtgg tctcgggatt ggtgaaccgg ctcaggcggt
     1141 cgcgcatgtg accgaacatg aaatacagcg acaccacgcc tccacaggcc gcagcggcca
     1201 acacggcgac ccacttcagc ggaaccccgg ccatgaagaa gacggccatg aaggtggtgg
     1261 tgatcagaag ggtctggcca atgtcgggct ggatcagcag caggccgacg accagcgccc
     1321 agaagccgaa cgcgatgcta acgccgggga cgccttgacc cttctgcccc tcggcgaaca
     1381 tccatgaggc gaagacgatc aggcagggtt tgacgaactc ggacggttgc aggctgaacg
     1441 ggccgaggtt gatccagcgc gccgcgccct tcacctcgtc gccgatgaac ggcagggcgg
     1501 ccatgacgac gatggcgccc agcagcccga tcacggccag acggcgcacc cctttgggcg
     1561 agaacaacga gaccgtcagc atcagcccca cgccgcccag ggcccagacg atcatccgcc
     1621 aggcatagtg gaacgggtcg gtgatcgact cgtctgccag gatggcggcg ggcgacgagg
     1681 cgaagctcag cgccacgccc atcgacatca ggatcaacgc cgcacccagc aggccgcgat
     1741 ccaccgtcca gaaccactgg gcgatcgggc tctggtcgtt gcgcgagaat gcggggctgt
     1801 agctggggct ggtcatcgtt cgctaaccat gaaccgaagg ggtagctccg aggttaagaa
     1861 ccgccgcacg gaacgcttct ccccgtgcct cgaagtcggg atattgatcg aacgacgcgc
     1921 aggccggcga caacagcacg atctgctcgc ctccagcggc tgctgcgtcc tcggcggcgg
     1981 cggccacggc ggcctccatc gtcttcgaga tgatgtgtgg ggtgtcgccc agcgttgcgg
     2041 cgaacgcctc ggccgcctcg ccgatcagat aggccttggt cacccgtccg aacaggtcgg
     2101 tcaggtcgtc gatgcccccg tccttggcct tgccgcctgc gatccagaat
//
LOCUS       DASVTM010000008         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_745644,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000008 DASVTM010000000
VERSION     DASVTM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745644"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..1082
                     /locus_tag="VF633_00200"
     CDS             381..1082
                     /locus_tag="VF633_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8469509.1"
                     /translation="MRASVGNRAIIACFYLQEDTMRATKPFTFGAMSRRAALTGAALT
                     AMVSLSACGGDKIASAEGDMGLGLPDGAKVTVVEYASVTCGHCAAWQAEVWPAFKAKY
                     VDTNKIRYVFREFPTAPVPVAAAGFLVARCAGPDKYFDVLHEIMASQNEIFGGTPPRT
                     VLLRIAQGAGMSEAQFTACVTDEKASAALEKRVQAGLDAGVQGTPTFMVNGEKVADSS
                     LAGLSGKIDAALAKN"
     gene            1116..1745
                     /locus_tag="VF633_00205"
     CDS             1116..1745
                     /locus_tag="VF633_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8469510.1"
                     /translation="MTLTQKLTGSVVALLALALAACGGGSGKPAEGDMALGAAEGAKV
                     TVIEYASPTCPHCALWQKNTWPAFKAQYVDTNKVRYIFRELPTPPVEVATAGILLARC
                     AGPDKYFDVMHELMASQDEWRAGALSPSASLLKTANSFGFSQEQYTACITNPDNIAAL
                     EKRIKAATDAGVNGTPIFKVNGVDVITPGSDEGTKLPDLAKAIDAELAK"
     gene            1750..>2012
                     /locus_tag="VF633_00210"
     CDS             1750..>2012
                     /locus_tag="VF633_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8469511.1"
                     /translation="MHLIQRLFLVLMLATSPLAVASVASAAELPPVTANDRVLGRADA
                     PVMVVEYASFTCSHCADWHKDVFPAFKARYIDTGKVRLVYRNLP"
BASE COUNT          383 a          703 c          599 g          327 t
ORIGIN      
        1 gcagcgggag cgaagatcaa ggcggtgagg gcggcgagga tcgacaccga agttttcaga
       61 cgcaggcgaa acgcgaaagg cacgaagcct ccttggaaaa tgtgaaatta tgcccgcagg
      121 cagatcaacg aagccgggta cgatccggaa cgtttctggc agatcgggag gacggagctg
      181 tccgcagcca cttctgcacc ctatagctgc tatagggtca agggttaagc cagtgactgc
      241 tttccacctc aacgacctca gccacgaccg gtttcggccc tcacgatcca atcgggtctt
      301 gaccgcttgg ctgtcctgcc ctgattggga tagcaacagt cgcggcccca tcttcgccgc
      361 acctgttctc gacaaacctg atgcgcgcct ctgtggggaa ccgcgcgatc atcgcctgtt
      421 tttacctcca ggaagacacc atgcgtgcga ccaagccttt tacattcggc gccatgagcc
      481 gccgggccgc cctcaccggc gccgccctga ccgccatggt ctccctgtcc gcatgcggcg
      541 gcgacaagat cgcatcggcc gagggcgaca tgggcctcgg cctgcccgac ggtgccaagg
      601 tgacggtggt cgaatacgcc tcggtgacct gcggacactg cgccgcctgg caagccgagg
      661 tctggcccgc gttcaaggcg aaatacgtcg acaccaacaa gatccgctac gtcttccgcg
      721 agttcccaac cgcgccggtg ccggtcgctg cggcgggctt cctcgtcgcg cgctgcgccg
      781 ggccggacaa gtatttcgac gtcctgcatg agatcatggc cagccagaac gagatcttcg
      841 gcggcacacc accgcgcacc gtcctgctgc gcatcgctca gggcgcaggc atgtcggaag
      901 cccagttcac cgcctgcgtg accgacgaga aggcttccgc tgcgcttgaa aagcgggttc
      961 aggccggtct cgacgccggg gtccagggaa cgccgacctt catggtcaac ggcgagaaag
     1021 tcgccgactc cagcctcgcc ggcctgtccg gcaagatcga cgccgcccta gccaagaact
     1081 gatttgacca gaaccgatcc tgaaagaacc gcatcatgac gctgacgcag aaactgaccg
     1141 gatcggtcgt cgccctcctc gccctggccc tcgcggcctg tggcggcggc tccggcaagc
     1201 ccgcggaggg cgacatggct ctgggcgcgg ccgagggcgc caaggtcacg gttatcgaat
     1261 acgcctcgcc gacctgcccc cactgcgccc tgtggcagaa gaacacctgg cctgcgttca
     1321 aggcgcagta tgtcgacacc aacaaggtcc gctacatctt ccgcgaactg ccgacgccgc
     1381 cagtcgaggt cgctacggca ggcatcctgc tggcccgctg cgccgggccg gacaagtatt
     1441 tcgacgtcat gcacgagctg atggccagtc aggacgaatg gcgcgcgggc gcgctgtccc
     1501 ccagcgcctc gctgctgaag acggccaatt ccttcggctt cagccaggaa caatacaccg
     1561 cctgcatcac caaccccgac aacatcgccg ccctcgaaaa gcgcatcaag gccgctaccg
     1621 atgcgggcgt gaacggcaca ccgatattca aggtcaacgg cgtcgacgtc atcacccccg
     1681 gcagcgacga aggcacgaaa ctgcccgatc tcgccaaggc catcgacgcc gaactggcga
     1741 agtagcacca tgcacctgat ccaacgcctc ttcctcgtgc tcatgctggc gacctcaccg
     1801 ctcgcggtcg cgtccgtcgc gtcggcagcc gagttgccgc ccgtcaccgc caacgaccgc
     1861 gttctgggtc gcgccgacgc tccggtgatg gtggtcgagt acgcctcctt cacctgctcg
     1921 cactgcgccg actggcacaa ggacgtcttc ccggccttca aggcgcgcta tatcgacacc
     1981 ggcaaggtgc ggctggtcta tcgcaacctg cc
//
LOCUS       DASVTM010000009         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_160600,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000009 DASVTM010000000
VERSION     DASVTM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160600"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..805)
                     /locus_tag="VF633_00215"
     CDS             complement(20..805)
                     /locus_tag="VF633_00215"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX8469512.1"
                     /translation="MAAALRAGPWRWLFLASALAWTATLALATPVPLSALCGAGSPDI
                     GRGVGAITAGLALVDLRELGLGWTIMIAAMMAPLLADPIVHVRQRSLRRRRRRGVLLL
                     CLGYFAIWMLCGIPLLALVVGVHAAMPVGLALPAALAVMALWLISPLRRLALQRCHRR
                     YPMRLSGLGADVDCFAHGLLTGAACAGSCWPVMAAAMLAPQHHVAMALAGVLLWLERL
                     RLLRLPAFAMRSPTANGPVRDARVARFDGSQIPAARLASGRPT"
     gene            complement(816..2270)
                     /locus_tag="VF633_00220"
     CDS             complement(816..2270)
                     /locus_tag="VF633_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX8469513.1"
                     /translation="MRDNPLSDRTSWAFLAAMHGFDEGLWNVHGMPTTPLPPRDDRKL
                     FWDQCQHLTWFFLPWHRGYLAAFEAILRDHIVRLKGPADWSLPYWNYHGGEERATWLP
                     PAFADETWPDGGPNPLKVSRWGANADDASGPTVDITRAEQRTALRTPNFASDDEQAGG
                     APGFGGPVTDFIQYGPAFGEAERSPHGLVHTMIGGFVRDGSGRRVDGIMSDPETAGYD
                     PIFWLHHCNIDRLWSVWMRRNAAHLNPVDLRWTEGPLIRRFTMPAIDGSAYEFVAGAM
                     VDSTVDALGYDYDDVADPLGGGSRLSARLLRAGVGELGDMALAQGPAERIGESGSLTL
                     TGRRAAATVAIDPIEGEAVASGIRSLRAGRLPKGTEPDRIFLNLEGIKSRRDSLEVDV
                     YVNLPAGVAPEDHPEAYAGTLSFFGVRKASLGNAEHGGNGVTQVLEITELVDELVITS
                     DRLARLEVELVANDEIVGDEVVSIDRVSVYRQRP"
     gene            complement(2464..2781)
                     /locus_tag="VF633_00225"
     CDS             complement(2464..2781)
                     /locus_tag="VF633_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469514.1"
                     /translation="MSVHDSRNWKATESTDFAGGNRGLTVTGDVQVGAGNQRPLLNRT
                     VPQGFNPSILYLDLSTETAGDVGTDALVWRTVSYEEPIEEDQFAQVDVRGAAVFDVEK
                     LIS"
     gene            complement(3431..3505)
                     /locus_tag="VF633_00230"
     tRNA            complement(3431..3505)
                     /locus_tag="VF633_00230"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3471..3473),aa:Val,seq:gac)
     gene            complement(3552..>3761)
                     /locus_tag="VF633_00235"
     CDS             complement(3552..>3761)
                     /locus_tag="VF633_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8469515.1"
                     /translation="EMDGIELAQRAGEMVSGLRVMFITGFAAVTLKAGREVPHARVLS
                     KPFHLRDLVLEVDRMFEVDSATGLN"
BASE COUNT          747 a         1222 c         1206 g          586 t
ORIGIN      
        1 cagcggccat gaagcctcct caagtcggtc tacccgatgc taggcgcgcc gcggggatct
       61 gtgaaccatc aaatcgggcg acgcgtgcgt cgcgtacggg cccgttcgca gtcgggctcc
      121 tcatcgcgaa cgcaggcagg cgcagaaggc gcaggcgctc gagccacagc agcacgcctg
      181 ccagagccat cgcgacatgg tgctggggag cgagcatcgc ggcggccatg accggccagc
      241 aggacccggc gcaggcggcc ccggtgagca gaccgtgggc gaagcagtcc acgtccgcgc
      301 ctaggccgct cagccgcatc ggataacggc ggtggcaacg ctgtagggcg agcctgcgca
      361 gcggcgagat gagccacagg gccatgaccg ccagagccgc cggaagtgca aggccgacgg
      421 gcatcgcggc atggacgccg acgaccaggg cgagcaaggg aatgccgcag agcatccata
      481 tcgcgaagta gccgaggcag aggagcagaa caccgcgtcg ccgccgccgc cgcaggctgc
      541 gttgccgaac atggacgatc ggatcggcga gcagcggcgc catcatcgcg gcgatcatga
      601 tggtccagcc aaggccgagc tcccgaaggt cgaccagcgc cagcccagcc gtgatggcgc
      661 cgaccccgcg gccgatgtcc ggcgagccag cgccgcacag cgccgacagc ggcacgggag
      721 tggcgagcgc cagcgtcgcc gtccaagcaa gagcggaggc gaggaaaagc caacgccacg
      781 gcccggcgcg gagcgccgcg gccaaggcgc gccgcttagg ggcgctggcg gtagacactg
      841 acccgatcaa tcgagacgac ctcgtcaccg acgatctcgt cgttggcgac tagctcgacc
      901 tcgaggcggg cgagccggtc gctggtgatg acgagttcgt cgaccagctc agtgatttcg
      961 agtacctgcg taacgccgtt ccccccatgc tcggcgttgc ctaggctggc cttgcgcacg
     1021 ccgaagaacg acagggtgcc ggcataggcc tcgggatgat cttcaggcgc caccccggcg
     1081 ggaaggttca cgtagacgtc gacctcgagg gaatctcgcc ggcttttgat gccctcgaga
     1141 ttgaggaaga tgcggtcagg ctccgtgccc ttcggcaaac gcccggcgcg cagggagcga
     1201 atgcccgacg caacggcttc tccttcgatc ggatcaatgg ctacggtggc ggcggcgcgg
     1261 cggccggtta gggtcaggct cccgctttcg ccgatgcgtt ccgccgggcc ctgcgccaat
     1321 gccatgtctc caagctcgcc gacgccggca cgcagcagcc gcgccgagag gcgcgacccg
     1381 ccgccgaggg gatcggcgac atcgtcgtag tcgtagccga gcgcgtcgac agtgctgtcg
     1441 accatcgcgc ccgcgacgaa ctcgtaagca ctgccgtcga tcgcgggcat ggtgaaccgg
     1501 cggataagcg ggccttcggt ccagcgcaga tcgacgggat tgagatgcgc ggcgttgcgg
     1561 cgcatccaga cgctccagag ccgatcgatg ttgcagtggt gaagccagaa gatcggatcg
     1621 tagccggcgg tctcgggatc ggacatgatg ccgtcgacgc gccggccgga tccatcgcgc
     1681 acgaaaccgc cgatcatggt atggaccagc ccgtgagggc tgcgctcggc ttcgccgaag
     1741 gccgggccgt actggatgaa gtcggtgacc ggtccgccga agcccggcgc cccgccggcc
     1801 tgttcgtcat ccgaggcaaa gttcggcgtc ctgagcgcgg tgcgctgctc ggcgcgggtt
     1861 atgtcgaccg tcggacccga cgcatcatcg gcgttggcac cccatcggct caccttgagc
     1921 gggttcggac ccccgtccgg ccaggtctcg tcggcgaagg cgggtggcaa ccaggtcgcg
     1981 cgttcctcgc ccccatggta gttccagtac ggcagcgacc agtcagcagg ccctttcagg
     2041 cgaactatgt ggtcacgcag gatcgcctcg aaggcggcga gatagccgcg gtgccagggg
     2101 aggaagaacc atgtcagatg ttggcactgg tcccagaaga gcttccggtc gtcacggggc
     2161 ggaagcgggg tggtcggcat cccgtgaacg ttccagagac cttcgtcgaa accgtgcatg
     2221 gcggcgagga aggcccagct ggtacgatcg gagagagggt tgtcccgcat gacctggacc
     2281 gcgcgggcgt accagattag ctcggacgac catcctggtt caaaactcca aacgctgcgc
     2341 cttgtccgca ccacagtccg tctcccaatg atggaaaatg cccggcgaag cccgccctga
     2401 agtcacccgc cccacggccc gcccggcgac agcgtgtcag gtcccaagcc agcgccgcaa
     2461 ggttcaggaa atcagcttct cgacatcgaa cacagccgca ccgcggacgt ccacctgggc
     2521 gaactggtct tcctcgatcg gctcctcata ggagacggtc ctccagacga gcgcatccgt
     2581 gccgacatca cccgcagtct cggtcgacag gtcgaggtac aggatcgagg gattgaagcc
     2641 ctgcggaaca gtgcgattca gcagcggcct ctgattgccc gcgccgacct gcacatcccc
     2701 ggtaactgtc agtccgcggt tgccgccagc gaaatcggtc gactccgtgg ctttccaatt
     2761 acgactgtca tgaacgctca tgctgccctc cttcgaacac aggcagacaa aggatgtcag
     2821 cctgactttc cttagctgaa cacgttaaag aaccacgata atcatgccgc atcaattgcg
     2881 agtcacgcaa acttacatgc ttgtcgcctc gctgtaaaca agaacaaaca tctcattcac
     2941 gaaggttaaa cttgtcaata gtcattggcg tcgacgaggc tcagcaatca aagacttatc
     3001 acaatttcag tgcggctcca tagcagtaga ttctggctaa ggctcttcag agcctagctt
     3061 ttccgataat cagcggcgcg cagatgacac ctctcgccaa ttatccaggt tgatatcagt
     3121 agcccaaagt ccggcagtag acgcactttc gaattcagca tctcgcccgt tcgcaagccg
     3181 cccatcgatg gaagtggaag gcgggcgggc gtccgtcctt accgaagcaa gcaggcgggt
     3241 cgccgcggct catacgcgcg ccgcgccgcc atgacaggcc gggacgaatg cccaacgtgc
     3301 ccagcctgtg cccagaagtc tgggcataat cagtcgaaat gaggtcagaa ctggtagaaa
     3361 cgggctctcg cccaagtctc attcaacaac aaaaaacccc cggaaatcag cagatttccg
     3421 gaggttttta tggtgggcgt ggcaaggatt gaacttgcga cccctgcgat gtcaacacag
     3481 tgctctacca ctgagctaca cgcccacgag gagggtgcct ctagccgcgg gttggccgcg
     3541 acgcaagcgg ttcaattcag accggtggcg ctgtcgactt cgaacatgcg atcgacctcc
     3601 aacacgaggt cgcgaaggtg gaacggcttg gacagcacgc gcgcgtgcgg aacctcgcgc
     3661 ccggccttca gggtcaccgc cgcgaagccc gtgatgaaca tgacgcgcaa gcccgacacc
     3721 atctcgcccg cgcgctgggc gagttcgatg ccgtccatct c
//
LOCUS       DASVTM010000010         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_693348,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000010 DASVTM010000000
VERSION     DASVTM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693348"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2132
                     /locus_tag="VF633_00240"
     CDS             <1..2132
                     /locus_tag="VF633_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469516.1"
                     /translation="APSVQLLSPNARNTAITIRGLGASYGLANDGLEQGVGIYIDQVY
                     YSRPATATLDFVDIDRIEILRGPQGTLFGKNTTAGALNITTRAPSFTPDAQGEITIGD
                     YGFVQAKATVSGPLIADTLAARLSFVSTQRDGVVDNVTGGHAQNAQKSISLHGQLLWE
                     VNERLRARLYADYSDQTPDCCTQLYVRYGKTQKPANLQFPALSAGLDYVPGSTNPYDR
                     IADVDGAIQADQIQRGVSAIIDYDFGFATLTSISAWRAWDWRPANDRDYTALDITRQS
                     ANPSDQDQWSQELRLSSNGKNRVAWTVGLYAFHQAVETHGITEYGRDASYWLTTVRDP
                     VTNALVPGTNPGLLDGYTVFNDSRIETDSLAVFGQVTWNITDRLHLTPGLRYTHEEKD
                     GAYASTVSGGGPGSAADLTRRLGVARPQAYAANISDGDLSGQVSLSYDINDNVLAYGS
                     LARGYKSGGINMAGIPNLPSGAPSLTNATVEPEVVTTYELGLKTQLFDKRVTANLAAY
                     RTDVEDYQANVVDTGPGALRGYLANAKRVEIQGIELDGFSRPTPWLDAYANVSWTDAK
                     YAAFSNGPCPLEQIGSTTVACDLSGKDLPGVSPWAGSLGGEVHRNVSGLGYTGEAYFG
                     ADASYRSTYNADASVSRYTEIEGYSLVNLRAGFRSDNGWEAFVFVKNALDEDYLQLLT
                     VQAGNSGLILGPPGDPRTVGLTLRAKY"
BASE COUNT          399 a          771 c          637 g          370 t
ORIGIN      
        1 tggctccgag cgtccagttg ctcagcccca acgctcgcaa cacggccatc acgatccggg
       61 gcctcggcgc cagttacggc cttgccaacg acggtctgga acagggcgtc ggcatctata
      121 tcgatcaggt ctattatagc cgcccggcga ccgcgacgct cgacttcgtc gacattgacc
      181 gcatcgagat cctgcgcgga ccgcagggga ctctgttcgg caagaacacc accgccggcg
      241 cgctcaacat caccactcgc gcacccagct tcacgccgga cgcccaaggc gagatcacca
      301 tcggcgacta tggcttcgtg caggccaagg cgactgtgtc cggcccgctg atcgccgaca
      361 cgctcgccgc acgcctgtcg ttcgtttcga cccagcgcga cggcgtcgtc gacaacgtca
      421 cgggcggaca tgcccagaac gcccagaagt ccatcagcct gcatggccag ttgctgtggg
      481 aggtgaatga acgcctccgg gcccgcctct acgctgacta ttccgaccag acccccgact
      541 gctgcaccca gctttacgtg cgctatggta agacgcagaa gcccgccaac ctgcagttcc
      601 cggccctgtc cgccggtctc gactatgtcc ccggcagcac caacccctac gaccggatcg
      661 ccgacgtgga cggtgccatt caggccgacc agatccagcg cggcgtctcc gccatcatcg
      721 actatgattt cggcttcgcc accctgactt cgatctcggc atggcgggcg tgggactggc
      781 gtcccgccaa cgaccgcgac tacacggcgc tcgacatcac gcggcagtcg gccaacccgt
      841 ccgatcagga ccagtggagc caggagttgc gcctgtcctc aaacggtaag aaccgagtcg
      901 cctggaccgt cggcctctac gccttccatc aggcagtcga gacgcatggg atcaccgaat
      961 acggccgcga cgcgtcctac tggttgacca ccgtgcgtga cccggtcacc aacgctcttg
     1021 ttcccggcac caaccccggc ctgctggacg gctatacggt gttcaacgac tcgcggatcg
     1081 agacggattc ccttgcagtc tttgggcaag tgacctggaa catcaccgac cggctgcacc
     1141 tgacacccgg ccttcgctac acccacgagg aaaaggacgg cgcctacgct tcgaccgtca
     1201 gcggcggcgg tcccggctct gcggccgatc tgacccgccg gctcggcgtc gcccggcctc
     1261 aggcttatgc cgccaacatc tcggacggcg atctgtcagg ccaggtctcg ctgtcctacg
     1321 acatcaacga caacgtcctg gcctacgggt cgcttgcacg gggctacaag tcgggcggca
     1381 tcaacatggc gggcataccg aacctgccgt ccggcgcgcc gtccctgacc aacgcgacgg
     1441 tcgagcccga ggtggtcacg acctatgaac tcggcctcaa gacccaattg ttcgacaagc
     1501 gcgtaaccgc aaacctcgcg gcctatcgca ctgacgtcga ggattatcag gccaacgtgg
     1561 tcgacaccgg ccccggagcc ctgcgcggct atctggccaa tgccaagagg gtcgagattc
     1621 aaggcatcga actggacggc ttttcccgcc cgacgccttg gctggacgcc tatgccaacg
     1681 tctcgtggac ggatgcgaaa tacgccgcct tcagcaatgg cccctgcccg ctggagcaga
     1741 tcggatcgac caccgtggcg tgcgatcttt ccggcaagga cctgccgggc gtttcgccct
     1801 gggccggatc actcggcggc gaagtgcatc gcaacgtcag cggcttgggc tacacgggtg
     1861 aggcctattt cggcgccgac gccagctatc gctcgaccta caacgccgac gcctcggtct
     1921 cgcggtacac cgagatcgaa ggctattcgc tggtcaacct acgtgctggg ttccgctccg
     1981 ataacggctg ggaagcgttc gtcttcgtca agaacgccct ggacgaggac tatctgcaac
     2041 tgctgaccgt gcaggctggc aactccgggc tgatcctcgg cccgcccggc gatccccgga
     2101 ccgtcgggct tacgctgcgg gcaaaatact gaggtcaggc tgaacgacac ccggcttgtt
     2161 tatgaggtcc tcgagcg
//
LOCUS       DASVTM010000011         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_958146,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000011 DASVTM010000000
VERSION     DASVTM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958146"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..480)
                     /locus_tag="VF633_00245"
                     /pseudo
     CDS             complement(124..480)
                     /locus_tag="VF633_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285154.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            605..>1697
                     /locus_tag="VF633_00250"
     CDS             605..>1697
                     /locus_tag="VF633_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8469517.1"
                     /translation="MSPQNALTVGVDFGTTNTVIAVTDAAGRAEVLRFQAPTGEVTAF
                     RSTLGFQIHPGADGRSPERVVTAGPWAIDAYVEDPFDTRFIQSFKTFAASAAFTETVI
                     DNRRYKFEDLLAAFLFQLKSHGGEATDDLPTQVIVGRPVTFAGGSPDERLALTRYEAA
                     FARLGFDDIRYAHEPVGAAFFFARDLKERATVLVADFGGGTSDFSIVRFEPTPDGLRS
                     EALARSGVGVAGDAFDYRIIDNLVSPELGKGGQYHAVGKTLPIPQRYYAAFARWDQLA
                     LLRASRDMKDIRSLARAAIEPEKLSRLVEVLDDNHGYALYQAVSRLKFALSQHVEATF
                     SFVAGSVTIEKPVSRAEFEGWIAPELTAIE"
BASE COUNT          292 a          522 c          548 g          335 t
ORIGIN      
        1 cggcgaccgc ccgttgaacg acagacatcg atttcctcaa cccagaaccc gccgatcttc
       61 gtcggccaga tcacagacta ggcgcacttt gacaccggtg tcgtcaagat tttcccggcc
      121 ctttcaggag cggatatcga cgtcttgcct tcttggccag caggaagccc agcacggcgg
      181 tcgctacggc gatcacgatc aggtaccaga gttcgtagca gatattgcac gtagcggcga
      241 ccatggcgaa gcccgtcgtc ggcaggaagc cacgaaccaa ccatttgccg atgtgatagg
      301 gcagggacat cgaggtgtag cggatgttgg tcgggagcat ctcgacccgc gccgccgcga
      361 caggcccgta gaccatcgtc acatagagac gagcaggaac aggatgcccg tggtcagtgg
      421 attgttgacc agagcgccgg ccggcttggc gggatagccg gcggctttca ggtgggcgcc
      481 ggtgatcggc tcttcgatcc tggccccgcc tgtcgatcac ggcttgccgc catctcatct
      541 atgaggcggg cgctggttgt ggattcgcgg taggaacccg ccccaccgtt gcagagtgct
      601 tgccatgtcc ccccagaatg ccttgaccgt cggcgtcgat ttcggaacca ccaacaccgt
      661 catcgcggtg accgatgcgg cgggtcgagc cgaggtgctg cgcttccagg cgccgaccgg
      721 ggaggttacg gcgttccgct ccaccttggg gttccagatt cacccggggg cggatgggcg
      781 ttcgcccgag cgcgtcgtaa cggcgggccc gtgggcaatt gatgcgtatg tggaagaccc
      841 cttcgacacg cggttcatcc agtcctttaa gaccttcgcc gccagcgcag ccttcaccga
      901 gacggtcatc gacaatcgtc gctacaagtt cgaggatctg ctggccgcgt tcctttttca
      961 gctcaagtca cacggcggcg aggcgacaga tgacctgccc acccaggtga tcgtcggtcg
     1021 tcccgtgacc ttcgctggcg gatcgcccga tgagcgcctg gctctgacgc ggtatgaggc
     1081 agctttcgcc cggctgggct ttgacgatat ccgctatgcc catgagcctg tcggtgcagc
     1141 cttctttttt gcccgcgacc tgaaagagcg ggcgacggtg ctcgtcgcgg acttcggggg
     1201 cgggaccagc gacttctcca tcgttcgctt cgagcctacg ccggacgggt tgcgttccga
     1261 agccctcgcg cgctctggcg ttggtgtggc gggcgacgcc ttcgactatc ggatcatcga
     1321 caatctggtt tcgccggaac ttggaaaagg gggtcagtat cacgccgtcg gcaagaccct
     1381 gccgatcccg cagcggtact atgccgcctt cgcgcgctgg gatcagctgg ccctgctgcg
     1441 ggcgtcgcga gacatgaaag acatccgcag tctcgctcgg gccgcgattg agcctgagaa
     1501 gttgagccga ctggtggagg tgctggacga caatcacggg tatgctcttt atcaagcggt
     1561 ctcgcggctt aagttcgcgc tgtctcagca cgtggaggcg accttctcgt tcgtggcagg
     1621 ctcggtaacg atcgaaaagc ctgtgtcgcg agcggagttc gagggttgga tcgccccgga
     1681 gctaacggcg atcgaga
//
LOCUS       DASVTM010000012         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_320434,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000012 DASVTM010000000
VERSION     DASVTM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320434"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VF633_00255"
     CDS             <1..1216
                     /locus_tag="VF633_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8469518.1"
                     /translation="TLHNTPQAGQSGAATWEPGSTEARSGVNVWGFMTVDEARGIVYV
                     PFGAPSWDRYGGDREGANLYANSIVAADAKTGKYLWHFQVTHHDVWDFDPASPPTLID
                     VQKDGKTIPAVAVVGKIGFMFILDRVTGEPIFKVEEKPVPGSDVPGEVVSATQPIPVL
                     PPPLSRVTVSEADLSHLTPEHEAFCAALVKENNLLLGGPYLPTALNRLTVNMPGTLGG
                     TNWGGGAFDPSTGLYIVNAFDLGQIQSLMPAPEGSATAYTNRSALFGRFWQPATRMPC
                     QAPPWGELLAVNVSTGQIAWRSTLGVTDTLPAAIQKTGRPSIGGPIVTAGGVVFIGAT
                     DDQRFRAFDAKTGAELWSTRLPSSAHATPMTYLGKDGRQYVVITSSGGSFLASPVNGD
                     EVTAYALPTAAQ"
     gene            1266..2225
                     /locus_tag="VF633_00260"
     CDS             1266..2225
                     /locus_tag="VF633_00260"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX8469519.1"
                     /translation="MIKFKTGLLLAAVALSFGTAAMAQSAPEPCASPRPAMQGPPGGP
                     GGPGGARPPRADPYAGKKKVLFIGDTLTGNQGAHDSVYHAMAVMQQLGAANDIAVFIR
                     TDWNNVTSGDVYGTVDYARCGPKESKGRNLSFFDAIIFYTNGETRLTDAQKADMMAWI
                     KSGKGFIGIHTATATGVYWPEYGQMIGGYFDNHPWGVTDAKVIVERPDFPGMAAFVQS
                     PNLRDEFYQMTGEPYSRENTDVLARLDISGLDMTNPQVHRTDGDFPVAWIRNYGEGRV
                     FYSDLGHPDSAWDDPRVANLYLEAMKWVTRVTDCPVRPHPIAN"
     gene            2242..>2570
                     /locus_tag="VF633_00265"
     CDS             2242..>2570
                     /locus_tag="VF633_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8469520.1"
                     /translation="MTTMSKPVRWGVLGAAQIAVNRAIPAMQAAENCEVVAIASRSLD
                     KAQGVATALGIPRAYGSYQELIDDPDVEAVYIPLPNTLHVEWSAKAMAAGKHALCEKP
                     IAMNAAEA"
BASE COUNT          451 a          906 c          792 g          421 t
ORIGIN      
        1 gacgctacac aacacgccgc aagccggtca gtcgggcgcc gccacctggg agccgggcag
       61 caccgaggcc cgctcaggcg tcaacgtctg gggcttcatg actgtcgatg aggcgcgtgg
      121 catcgtctat gtgccgttcg gcgccccctc gtgggaccgc tacggcggcg accgtgaagg
      181 cgcgaacctg tacgccaaca gcatcgtcgc ggccgacgcg aagacgggta agtacctctg
      241 gcatttccag gtcactcacc acgatgtctg ggacttcgac ccggcctcac caccgaccct
      301 gatcgacgtc cagaaggacg gcaagaccat ccccgccgtc gcggtggtcg gcaagatcgg
      361 cttcatgttc attctcgacc gcgtgacggg cgagccgatc ttcaaggtcg aggaaaagcc
      421 ggtccccggc agcgacgtgc ccggcgaagt cgtgtcggcc acccagccca tccccgtgct
      481 gccgccgcct ctgtcgcgcg tgacggtaag cgaagccgac ctctcccacc ttacgccgga
      541 gcatgaggcg ttctgtgcgg ctctggtgaa ggagaacaac ctgctgctcg gcgggcctta
      601 cctgccgacc gcgctgaatc gcctgacggt caatatgccc ggcacgctgg gcggcaccaa
      661 ctggggcggc ggagccttcg atccgtcgac gggcctctac atcgtcaacg cctttgacct
      721 cggccagatt cagtccctga tgccggcgcc cgagggcagt gcgaccgcct acacgaaccg
      781 ttcggccctg ttcggccgct tctggcagcc ggcgacgcgt atgccttgcc aggccccgcc
      841 ttggggtgag ttgctggcgg tcaacgtctc gacgggccag atcgcctggc gcagcacact
      901 cggcgtcacc gacaccctgc ccgcagccat ccagaagacc ggtcgcccca gcatcggcgg
      961 gccgatcgtg accgcgggtg gcgtggtctt catcggcgcc accgacgacc agcgcttccg
     1021 ggctttcgac gcaaaaaccg gcgcggagct gtggagcacg cgcttgccgt cctcggccca
     1081 cgccacgcct atgacctacc tcggcaagga cggtcgtcag tacgtcgtca tcacctcgtc
     1141 gggcggcagc ttcctcgcca gtccggtcaa cggggacgag gtcaccgcct acgccctgcc
     1201 gacggctgcc cagtaaacgt cagtaatccc tttcgcctcg ttcaatcctc ttcaggagtc
     1261 gtcgcgtgat caagttcaag accggcctgc tgctggccgc cgtcgccctt tcgttcggca
     1321 cggccgcgat ggctcaaagc gccccagagc cctgtgcgag tccccgcccg gcgatgcagg
     1381 ggcctccggg tggtcccggc ggtccgggcg gcgcacgtcc gccgcgcgcc gatccttatg
     1441 ccggcaagaa gaaggtgctg ttcatcggcg acaccctgac cggcaaccag ggcgcccatg
     1501 actccgtcta tcatgccatg gcggtcatgc agcagctcgg cgcggccaac gacatcgccg
     1561 tcttcatccg caccgactgg aacaacgtca ccagcggaga cgtctacggc accgtcgact
     1621 acgcccgctg cggccccaag gaatcgaagg gccggaacct gtccttcttc gacgccatca
     1681 tcttctacac caacggcgag acgcggctga ccgacgccca gaaggccgac atgatggcat
     1741 ggatcaagtc aggcaagggc ttcatcggca tccacaccgc gaccgcaact ggcgtctatt
     1801 ggcccgaata cggccagatg atcggcggct atttcgacaa ccatccgtgg ggtgtcaccg
     1861 acgccaaggt tattgtcgag cggccggact ttcccggcat ggcggccttc gtccaaagcc
     1921 cgaacctgcg cgacgagttc tatcagatga ccggcgagcc ctattcgcgc gagaacaccg
     1981 acgtgctggc tcgactggac atcagtggtc tggatatgac caacccgcag gtccaccgta
     2041 ccgacggtga tttcccggtc gcctggatcc gcaactacgg cgaaggccga gtcttctact
     2101 ccgacctcgg ccacccggac tcggcctggg acgatccacg cgtcgccaac ctttatctgg
     2161 aagcgatgaa gtgggtgacg cgggtcaccg actgtccggt ccgcccacac ccaatcgcga
     2221 actgacctgt ccgacgcggc tatgaccacc atgtcgaagc ctgtgcgctg gggcgtgctg
     2281 ggggccgcgc agatcgcggt aaaccgcgcg atcccggcga tgcaggcggc ggagaactgc
     2341 gaggtcgtgg ctatcgcgtc gcgtagtctc gacaaggctc agggtgtcgc gactgctctg
     2401 ggcatcccgc gcgcttacgg cagctatcaa gagctgatcg acgatcccga cgtcgaggcg
     2461 gtctatatcc cgctgccgaa caccctccat gtcgaatgga gcgccaaggc catggccgcc
     2521 ggcaagcacg ccctgtgcga gaagcccatc gccatgaacg ccgcggaagc
//
LOCUS       DASVTM010000013        10919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_852080,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000013 DASVTM010000000
VERSION     DASVTM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852080"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1001
                     /gene="hisN"
                     /locus_tag="VF633_00270"
     CDS             216..1001
                     /gene="hisN"
                     /locus_tag="VF633_00270"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX8469521.1"
                     /translation="MTEFEAFAVELAREAAKVTLPFFRGIYSEENKAGPGSFDPVTQA
                     DKDAEAAIRRLIAARYPDHGVIGEEYGEDRPDAEHVWILDPIDGTRAFIAGLPLWTTL
                     IGLRVAGNPAVGVIAQPYLDEIFIGGPSGAALIRESGATSLKVRACERLTDAVISTTD
                     PEIFSGSELGAWTQVRAAARLARYGCDAYAYAMVAAGRIDLVAEASLKLWDWSALIPV
                     VEAAGGEVTNWRGEAVDGTGQILAVGDVRIREQALVTLRRASL"
     gene            complement(998..2407)
                     /locus_tag="VF633_00275"
     CDS             complement(998..2407)
                     /locus_tag="VF633_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX8469522.1"
                     /translation="MTQDVSYFFCGIGGSGMLPLAMIVQAQGNAIEGSDRALDQGRTP
                     AKFDWLRAHGVTLHPQDGSGVTRAEQIVVATGAIEDTVPDIGAARRAGATIVTRPQLL
                     SQLFNAAPTSVGVAGTSGKSTITGMVAWILHSASRAPTVMNGAVMKNFADADHPFASA
                     LIGGADLFVAEVDESDGSIARYDPTVAVVSNISLDHKSMEELRDLFGGFISRAQTAVL
                     NLDNVETAALAQSMVEDARGDVVVTFALGNDDADLVAHALSSLPTGMTFDLTERSSGY
                     LFKVVLNVPGAHNVANALAALGAVRALGVPLAEAVSALESFSGIRRRMEVVGTKNGVT
                     VIDDFAHNPDKIAATMKTLHAFDGRLLILFQPHGFGPLKLMKQEFIEGFAGLLRPDDV
                     LLMPEPVYYGGTTDRSVGSEDIAAGVRALGRNAEALHDRPACGDRLIELARPGDRIIV
                     MGARDDTLSEFAAELLARL"
     gene            2543..3373
                     /locus_tag="VF633_00280"
     CDS             2543..3373
                     /locus_tag="VF633_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX8469523.1"
                     /translation="MTANVFKIGIVCVSSRFAKERAEAVEAWMAQHHPDGDVVVTFHP
                     ACFMKHGHFAGDDRARADAFVEYANDPRFDAIWFARGGYGSCRIVEDVIPRLNDVARG
                     KRYLGYSDVGTLLAGMYKAGFKHLAHGPMASDSVRHDATAERALTWLVKGDEASLEPS
                     LAIDKKKAVAFNLTIIDQLVGTPLEPDLTDHVLMIEEVSEALYRVDRMMFHLTSQPSI
                     RKVAGIRLGRVSDVTVNDPDFGLTAQEIVRYWCQRSGIPFLGKADIGHDGDNKVVPFG
                     "
     gene            complement(3682..4884)
                     /gene="metB"
                     /locus_tag="VF633_00285"
     CDS             complement(3682..4884)
                     /gene="metB"
                     /locus_tag="VF633_00285"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX8469524.1"
                     /translation="MTASPRNPARSAKDIRTICARSGVDQDPAHGAVMPPLYLSSNYS
                     FAGFDQKRKYDYSRSGNPTRDVLADTLAELEGGAGCVVTATGMAAVDLPLSLLSPGDL
                     LIAPHDCYGGTHRLLCAHAKKGHFEVLWVDQGDPEALAAALALNPKLVLVETPSNPLL
                     RVVDVADICVRAHAVGAKVIADNTFLSPALQRPIELGADIVVHSTTKYINGHSDVVGG
                     AVISADPADHAELAWWANCRGVTGGAFDAYLTMRGIRTLFTRIERQQETAGHVAALLE
                     AHPAVKAVHYPGLKSHPNHALAARQQHGPGAMLSFELVGGVEAVRTLVETIETFTLAE
                     SLGGVESLIAHPATMTHAAMTPEARVVAGITDGLLRVSVGLEHQDDLIADLVRALDAV
                     SVEQAEAA"
     gene            complement(4881..5987)
                     /locus_tag="VF633_00290"
     CDS             complement(4881..5987)
                     /locus_tag="VF633_00290"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEX8469525.1"
                     /translation="MQTTMAFDTETTEPNCTAKATLTPGIPLESRPHNFVPSLRRSRQ
                     GNAVDITAAIPSDFRLQSGERLNQTTVTGRLHGREGAPLVVVAGGISAGRFVHRTETN
                     GLGWWSDAVTAGGPIDLRRCQVLAFDYAPGADNESAADGVSVTITTHDQARLLALLLE
                     QIGAETVAAIVGCSYGGMIALAFGELFPRWAEQLIVVSAAHRAHPQATAWRGIQRRIL
                     KLAAEAGRPEDGVALARELAMTTYRTAEEFGERFETAAPLAAGQAYPVCEYLTARGKA
                     YRTHTTPWRWLSLSDSIDRHAVTPENIVAPVTVVGFTSDRLVPIDDIRELAARLPTLW
                     RLVEAPSLYGHDAFLKEDALVGDILRSALKDIDQ"
     regulatory      complement(6054..6164)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6355..6489
                     /locus_tag="VF633_00295"
     CDS             6355..6489
                     /locus_tag="VF633_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469526.1"
                     /translation="MSRSGLFAISISTAAVVTARTGTPAFTTTGGMTTITGLSGAGTG
                     "
     gene            6603..8348
                     /locus_tag="VF633_00300"
     CDS             6603..8348
                     /locus_tag="VF633_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8469527.1"
                     /translation="MPQLSVVQNSEPLKPVAEQSDAFDIVVLKFGSSILRSQSDAPAV
                     ASEVYGHVRAGRKVVAVVSAFGGHTDTLLADARLLGLDHENDLLPGYVALGEEQAAAL
                     TAIACDRIGLDAVALSVRELGIIAEGPSEHAHPCGLKSEALLRALADHQVVVVPGFGA
                     VRADGKVALLGRGGSDMTAVFLAAELGLSRVRLVKDVDGLYDRDPAAASGKPLRYRRA
                     SWDDARRYGGALVQHDAIDLGQERAVEIEVAGLGRAGGTVVGDHSAPPGPSVPDAPIR
                     VAIAGCGVVGGGLLARLLTDPRYEVTGVLVRDPKKVRDVAAPADLYTSDRAAFLAAKP
                     DLLVEALSEGEAGHDLIRAALEAGIDVASANKQAISRDPAGLQAWADKHKALLVYSAA
                     VGGGAPMIETLRVAREAGPVAGFEAVLNGTVNFMLDRLSAGADFADALADARVAGFAE
                     EDPSSDLEGRDAAAKVRLLSFEAFGETPKDGAITCDVLSAAFKPNGGPVRQIGACHKS
                     DAGLDASVVLDRKLEDPLFLALRGERNALKVYGEDGRVWSCKGRGAGRWPTTESVLAD
                     VADIIRARHAALGYH"
     gene            8352..9302
                     /gene="thrB"
                     /locus_tag="VF633_00305"
     CDS             8352..9302
                     /gene="thrB"
                     /locus_tag="VF633_00305"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX8469528.1"
                     /translation="MAVFTPVSDDQARAFVDGYPAAGEFVSLHAIAEGVENTNYRLET
                     SAGRFVLTLFEGRTDAASLPFCLGLTDHLGAGGYPAARPLRDRKGEWIGALNGRPAAI
                     VQWLTGAWLRTPSLDEVARAGAALARLHLAARDFAGRRANPVGPVVWKVLLEKSAVGA
                     VTAEDRELVAGCRLALERLGDPFTDDLPTGAIHADYFPDNVLFEHGQVSGVIDVYFGC
                     DGPFAYDLAIALTAWGFDDEGCEIAGQMEAFRRGYESVRLLTWAEERALLVLGETAAL
                     RFTLTRLHDRIFHDPTKLVTPKDPAAFFRRMAYWRVAETV"
     gene            complement(9299..10366)
                     /locus_tag="VF633_00310"
     CDS             complement(9299..10366)
                     /locus_tag="VF633_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469529.1"
                     /translation="MSSIRPDRALSVAWAISSERQPDAYESYRSTIADFYEVSGVADS
                     GRTGFRSQTTAWRFGATSFARGRSVPQTLARGAAEIRRSGLDQISIIVNLSDTLGDCD
                     GRAISAEAGSVQFRDLSRPSASRVETIDVINLVTPRTSVPSWLLGRKVHGLVLPSASP
                     GGRLVASHLKTLADVAGDLTEEEGVAAIEATFIISERFLGRAGPIAPMQMESIHRTIR
                     QRAMQVFDARASDPTLNVNEVALQIGVSRSALYRAFGDMGGVQTYVLHRRLDKAYADL
                     RVSGGGSLANIQGIAERYGFGSRARFDRAFRDRFGFRSIEVAPVGFAGRGAPLAANEE
                     GYLDGAAHDVVIDWLRRSEAA"
     gene            complement(10471..>10919)
                     /locus_tag="VF633_00315"
     CDS             complement(10471..>10919)
                     /locus_tag="VF633_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX8469530.1"
                     /translation="SNFERLVFEGSGRDAEATRAMFETFARDGQTALPADLLLAIRAE
                     VSAVSIDEATTRSEIAQAYETWGRVVCPHTAVALAAARRQPTGGPMIALSTAHAAKFG
                     KDVAPVLGFDPDPPHVIRELGDRPERLTLIDGTHEAALEAVRSFRR"
BASE COUNT         1820 a         3421 c         3780 g         1898 t
ORIGIN      
        1 caggcatatt atcagcttgg cgagcggcca cgtcgccgtc tgctggacct ggccaagtca
       61 cttcacaacg aagaacccgc cgcctgagtg gggcgcggcg agtgtcggat ggtcggcggc
      121 cgggtctctg aaagggacaa gggcgagcga catggggggt attgccggtc taaggacggg
      181 gataccgctg accacacttc acgaactgcg tatccatgac cgaattcgaa gccttcgccg
      241 tcgaactggc ccgtgaagcg gccaaggtga ccctgccgtt ctttcgggga atatactccg
      301 aggagaacaa ggccggaccg ggatcgttcg atcccgtcac ccaggccgac aaggatgccg
      361 aagccgccat ccgccgtctg atcgccgccc gctatccgga ccatggcgtc atcggcgagg
      421 agtatggcga ggaccgtccg gacgcggagc atgtctggat tctggacccc atcgacggca
      481 cccgcgcgtt tattgcgggc ctgccgctct ggaccacatt gattgggctc cgggtcgcgg
      541 gaaacccagc cgtcggggtg atcgcccagc cctatctgga tgagatattc atcggagggc
      601 cctccggggc ggcgctgatc cgggagagcg gcgccacgtc attgaaggtc agggcgtgcg
      661 agcgcctgac cgatgcggtc atctccacca ccgatcccga gattttcagc ggttccgaac
      721 tcggggcctg gacccaggtg cgggccgccg cccgtctcgc tcgctacggc tgcgacgcct
      781 atgcctatgc catggtggcg gccgggcgga tcgatctggt ggccgaagcc agcctgaaac
      841 tctgggactg gtcggcgctg atcccggtag tcgaggcggc cggtggcgaa gtcaccaact
      901 ggcgcggcga ggctgtcgac ggcacaggac agatcctggc cgttggcgac gtccgcatcc
      961 gtgagcaggc gctggtcacc ctcagacgcg cgtcgctcta gaggcgggcg agaagctcag
     1021 ctgcgaattc ggacagggtg tcgtcgcggg cgcccatgac gatgatgcgg tcaccgggtc
     1081 tggcgagctc gatcaaccgg tctccacaag ccggacgatc gtggagggcc tcggcgttgc
     1141 ggccgagagc ccgaaccccg gcggcgatat cttccgaacc cacgctgcga tcggtcgtcc
     1201 cgccgtaata gacaggctcg ggcatcagca ggacgtcgtc cgggcgcagc agtccggcga
     1261 agccttcgat gaactcctgc ttcattagct tcagcgggcc gaagccgtgg ggctggaaca
     1321 ggatcaacag ccggccatcg aaggcgtgca gggtcttcat cgtcgccgca atcttgtccg
     1381 ggttgtgggc gaagtcgtcg ataacggtga cgccgttctt cgtgccgacg acctccatcc
     1441 ggcgacgaat tcctgagaag ctttcaagcg ctgaaactgc ctcggccaag ggaacgccga
     1501 gcgcacgcac cgcgccgagc gccgccagcg cattggcgac attgtgcgcg ccggggacgt
     1561 tcaggacgac cttgaacagg tagccgctcg accgctcggt cagatcgaat gtcatccccg
     1621 ttggcaggga actcagggcg tgggcaacga ggtcggcgtc atcattgccg agggcgaagg
     1681 tgactacgac gtcgccacgc gcgtcctcga ccatcgattg ggcgagcgcc gccgtctcga
     1741 cgttgtcgag gttgagcacg gccgtctgcg cccggctgat gaagccgccg aacaggtcgc
     1801 ggagttcctc catcgacttg tgatcgagcg aaatgttgga aaccacggcg actgtcggat
     1861 catagcgggc gatcgagccg tccgattcgt ctacctcggc cacgaaaaga tcggcaccac
     1921 cgatcaaggc gctggcgaac ggatggtcgg cgtcggcgaa attcttcatc acggcgccgt
     1981 tcattacggt tggggcacgg ctggccgaat gcagtatcca ggcgaccatg ccggtgatgg
     2041 tggacttgcc gctggtgccc gcaacaccga ccgaggtggg ggcggcgttg aacaactggc
     2101 tcagcagttg aggacgggtg acgatggtcg cgcctgctcg tctggccgca ccgatgtcgg
     2161 gtacggtgtc ctcgatggcg ccggtggcga cgacgatctg ttcggcccgg gtcacgcctg
     2221 atccgtcctg tgggtgcagg gtcacgccgt gggcgcgcag ccagtcgaac ttggccggtg
     2281 tccggccctg atcgagcgcc ctgtcggagc cttcgatggc gttgccctgc gcctgaacga
     2341 tcatggccag cggcagcatg ccggagccgc cgatgccgca gaagaagtat gagacgtctt
     2401 gggtcatcag gccttagaac agggcttcgt cgcgaaccgc cagtgggttt gcgaaatgaa
     2461 tgcgtattgg agctctcgtg gcgcttgaaa tcgatccaga caagaccgct ctcgtcgccg
     2521 acgtcgtggc ggctctgcct gcatgacggc caacgtcttc aagatcggta tcgtctgcgt
     2581 cagctcgcgt ttcgccaaag agcgcgcgga ggctgtcgag gcgtggatgg ctcaacatca
     2641 tcctgacggt gacgtcgtcg tcaccttcca tccggcctgc ttcatgaagc atgggcattt
     2701 cgccggcgac gatcgggccc gcgccgacgc cttcgtcgaa tacgccaacg acccgcggtt
     2761 cgacgccatc tggtttgcgc gtggaggcta tggttcgtgc cgcatcgtcg aggacgtgat
     2821 cccgcgactg aacgacgtgg cgcggggtaa gcgctatctc ggatattccg atgtcggcac
     2881 gctgctggcc ggaatgtaca aggccgggtt caagcatctg gctcatgggc cgatggcctc
     2941 ggactccgtg cgtcacgacg caaccgccga gcgggcgttg acctggctgg tcaagggcga
     3001 cgaggcttcg ctggagccgt cgctggcgat cgacaagaag aaggcggtcg ccttcaacct
     3061 caccatcatc gaccagttgg tgggcacgcc gctcgagccc gacctgaccg accacgtcct
     3121 gatgatcgaa gaggtgtcgg aggcgctgta ccgggtcgac cggatgatgt tccacctcac
     3181 cagccagccg tcgatccgca aggttgcagg tattcgtctc ggccgggtct cggacgtcac
     3241 ggtgaacgac cccgactttg gtctgacagc gcaggagatc gtccgctact ggtgccagag
     3301 gtctggcatt ccattcctcg gcaaggcgga tatcggccat gacggcgaca acaaggtcgt
     3361 cccgttcggt taaatctcgc gccctcgcct cacgtcggac gcgagaatga tgcgcgcggg
     3421 acacgccggg ctcgcgtcgt taacgcccgt taacgtgggc ctatcgactc cggcgaattg
     3481 tcagggccag atgcaagtga tggagacctt tgacgcaccg ccttagcggt gcggctggtc
     3541 gtctcgggtt tacggcaggt ctggagattg cgtcgggggg cgccgctctg gccccggggg
     3601 tacaggctgt cgagagccgg agcttcatca ccagcgacgg ggccggcctt cattgctgaa
     3661 ggcgggcccc cgaccttgag actaggccgc ttccgcctgt tccactgata cggcatccag
     3721 cgcgcggacg agatcggcga tcaggtcgtc ctgatgctcg agaccgactg acacccgcag
     3781 cagtccatcg gtgatgccgg cgacgacgcg ggcttcggga gtcatggccg cgtgggtcat
     3841 ggtcgccggg tgggcgatca ggctttcgac accgccaagg ctctcagcga gggtgaaggt
     3901 ctcaatcgtc tcgaccagag tgcggacagc ctcgacgccg ccgaccagct cgaagctgag
     3961 catggcgccc ggaccgtgct gctgacgggc cgccagagcg tggtttgggt gcgatttcag
     4021 accgggataa tggacggcct ttaccgccgg gtgggcttcc agcagggcgg ccacgtggcc
     4081 ggccgtttcc tgctggcgct cgatgcgggt gaacagggtg cggatgccgc gcatggtcag
     4141 ataggcgtcg aaggcgccgc cggtcacgcc ccggcagttg gcccaccagg cgagttcagc
     4201 gtggtcagcc ggatcagccg agatcaccgc gccgccgaca acgtcggagt ggccgttgat
     4261 gtatttggtg gtcgagtgga cgacgatgtc ggcgccgagc tcgatcgggc gctgcagggc
     4321 gggcgacagg aaggtgttgt cggcgatgac cttggccccg acggcgtggg cgcgaacgca
     4381 gatgtcggcg acgtcgacga cccggagcag ggggttggaa ggggtctcga ccagcaccag
     4441 cttggggttc agggcgaggg cggcggcgag ggcttccggg tcgccctgat cgacccaaag
     4501 gacttcgaaa tggccctttt tcgcgtgggc gcacagcagg cggtgcgtgc cgccgtagca
     4561 gtcgtgcggc gcgattagca ggtcgccggg cgacagcagg gacagaggca ggtcgaccgc
     4621 ggccatcccc gttgcggtga cgacgcagcc ggcgccgcct tctagctcgg ccagcgtatc
     4681 ggccagcacg tcgcgcgtcg ggttgcccga ccgggaatag tcgtatttcc gcttctgatc
     4741 gaaaccggcg aacgaataat tcgacgatag atagagtggc ggcatgaccg cgccgtgggc
     4801 cggatcctga tcgacgcccg atcgggcgca gatggtgcgg atgtcttttg ccgagcgggc
     4861 ggggttacgc ggggaggcgg tcactggtcg atgtccttca aggcggagcg gaggatgtcg
     4921 ccgacgaggg cgtcctcttt cagaaaggcg tcgtggccgt acagggacgg agcctcgacc
     4981 agacgccaca gggtgggcag gcgggccgcg agttcgcgga tgtcatcgat cggcaccagc
     5041 cggtcggagg tgaagcccac caccgtgacc ggagcgacga tgttctcggg cgtgaccgcg
     5101 tggcggtcga tagagtccga cagcgacagc caacgccagg gcgtggtgtg ggtccggtac
     5161 gccttgccgc gcgcagtcag gtattcgcac acgggatagg cctgacccgc ggcgaggggg
     5221 gctgctgtct cgaaccgttc gccgaactct tccgccgtgc ggtaggtggt catggccagt
     5281 tcgcgggcga gagccacgcc gtcctcgggg cgaccggcct ccgcggccag cttgagaatg
     5341 cggcgttgga tgccgcgcca tgcggtggct tgaggatgcg cgcggtgggc ggcggagacg
     5401 acgatcagct gctcggccca gcgcgggaac agctcgccga acgccagggc gatcatgccg
     5461 ccgtaggagc aaccgacgat ggccgcgacc gtctcggcgc cgatctgctc aaggagcagg
     5521 gccagcagtc gggcctgatc gtgggtggtg atggtgacgc tgacgccgtc cgcagcggat
     5581 tcgttgtcgg cgccgggggc gtaatcgaag gccagaacct gacagcggcg caggtcgatt
     5641 ggcccgcccg cggtgacggc gtccgaccac cagccgaggc cgttggtctc ggtccgatgc
     5701 acgaaacgcc ccgccgaaat gccaccggcg acgacgacca gcggcgcgcc ttcacgcccg
     5761 tgcaggcggc cggtcacggt ggtctggttc agccgctcac ccgactggag gcggaagtcg
     5821 gacggaatgg cggcggtgat gtccacggcg ttgccctgac ggctgcggcg cagcgaaggc
     5881 acgaaattat gcggcctcga ctcgagcggg atccccggcg tcagggtcgc cttggcggta
     5941 cagttgggtt cggtggtttc agtgtcgaag gccatcgtcg tctgcatcaa tcctgctcag
     6001 caaggacccg atgcgatgac agatcgtgga gacgccgccg tagaccggcg ctccactcat
     6061 ctctcgcggt tccgaggaac ctcgcaggag ttggcaccgt tcgggcggac ccgatggttg
     6121 ccccgacgtc aaagggccta tccctcggtc ggtcttgatg agtgacttta ccgtgaggca
     6181 aaacaggggg attggtcaag cggaaaaatt cgccaaggcc cgggcaatcg gatacggctc
     6241 tgtgccgcgt tatactcgtc gtaaccgcta tgggcgcaaa aaacttccaa caaaggcttg
     6301 acggtggcgc tccggcccga caccctgagt caacttcatc gcaaggtttt caccgtgtcc
     6361 cgctctggcc tcttcgccat ttcgattagc accgccgccg tcgtgacggc gcggacgggc
     6421 acgcctgcgt tcaccaccac cggcggtatg accaccatca ccggcctctc aggggcgggg
     6481 acaggttagc gcgcgtcggc accgacaagc accaacccag acccgcccgg acccccgggc
     6541 gggttttttc ttgcccgttt cccccgtccc attcccttcc agcgccctga tccgagactg
     6601 aaatgcccca actctccgtc gtccagaact ccgagcctct caaaccggtc gccgagcaaa
     6661 gcgacgcgtt tgacatcgtc gtgctaaaat tcggatcgtc cattctgcgc tcccagagcg
     6721 acgccccggc cgtggcgtct gaggtctatg gccatgtccg cgccggtcga aaggtggtgg
     6781 cggtggtctc ggccttcggg ggtcataccg acacgcttct ggccgacgcc cgcctgctcg
     6841 gactcgacca tgagaacgat ctcctgcccg gctatgtggc gttgggcgag gaacaggcgg
     6901 cggcgcttac ggcgatcgcc tgtgaccgga tcggactgga cgcagtggct ctttcggtgc
     6961 gtgaactcgg catcatcgcc gagggaccat ccgaacacgc acacccctgc ggtctcaaaa
     7021 gcgaggcgct gctgcgggcg ctggcggatc accaagtggt cgtcgttccc ggcttcggcg
     7081 cggtacgagc tgacggcaag gtggcgctgc tgggacgggg cggctcggac atgacggcgg
     7141 tatttctcgc cgcagagctc ggtctgtcgc gggtacggtt ggtcaaggat gtggacggcc
     7201 tctacgaccg cgatcccgcc gccgcctcgg gcaagccgtt gcgttatcgt cgggcctcgt
     7261 gggacgacgc gcgacgttat ggcggagccc tggtccagca cgacgccatc gatctggggc
     7321 aggagcgcgc cgtcgagatt gaggtcgcag gactcggacg cgcagggggc acggtagtgg
     7381 gggatcattc cgcgcctccg ggaccctcgg tgcccgacgc gccgatccgg gtcgccatcg
     7441 ccggttgcgg cgtcgtcggc ggtgggcttc tggcccgatt actgaccgat ccacgctacg
     7501 aggtcaccgg cgtgctggtg cgcgatccga agaaggtgcg tgatgtggcg gcgcctgccg
     7561 acctgtacac ctcggacagg gcggcgttcc tcgccgccaa gcccgacctg ctggtcgagg
     7621 ctctttccga aggggaggcg ggccacgacc tgatccgcgc ggcgcttgag gccggcatcg
     7681 acgtggccag cgccaacaag caggcgatca gccgcgatcc ggccgggctt caggcctggg
     7741 cggacaagca taaggctctg ctggtttact ccgccgcagt cggaggtggc gcgcccatga
     7801 tcgagacgct gagggtggcg cgcgaagccg gtcctgtcgc gggcttcgag gcggttttga
     7861 acggcacggt caacttcatg ctcgaccggt tgagcgccgg agccgacttc gccgacgccc
     7921 tggccgacgc ccgggttgcg ggtttcgccg aagaggaccc atcgtccgat ctggaggggc
     7981 gcgacgccgc cgccaaggtc cgcctgctgt ctttcgaggc gttcggcgaa actccgaaag
     8041 acggcgcgat cacctgcgac gtgctgtcgg cagcgttcaa accgaatggc gggcctgtcc
     8101 gccagatcgg cgcctgccac aagtcggacg ccggtctcga cgcgtccgtt gtgctggacc
     8161 gcaagctgga ggatccgctg ttcctcgccc tgcgtggcga acgcaatgcg ctcaaggtct
     8221 atggtgagga cggtcgcgtc tggtcgtgca agggacgcgg cgccggtcgt tggccaacga
     8281 cggaaagcgt gctcgctgac gtcgccgaca tcatccgtgc gcgtcatgcc gccctaggct
     8341 atcactagat catggccgtc ttcaccccgg tttcggacga tcaggcgcgc gcgtttgtcg
     8401 acggctatcc ggccgcaggc gagttcgtgt cgctgcacgc catcgccgag ggggtggaga
     8461 acaccaacta tcggctggag accagcgcgg gccgcttcgt tttgacgttg ttcgaaggcc
     8521 gcacggacgc cgcttcccta cccttttgcc tcggtctgac cgaccatctt ggtgcggggg
     8581 ggtatccggc ggcgcgacct cttcgcgacc gcaagggcga atggatcggc gccttgaacg
     8641 gccgaccggc cgcgatcgtc caatggctga ccggcgcctg gttgcgtaca ccttcgctcg
     8701 acgaagtggc ccgggccggc gcggcgcttg ccagactgca tctggccgcg cgagacttcg
     8761 cggggcgacg ggccaacccg gtcggcccgg tcgtctggaa agtgttgctg gagaagtccg
     8821 ccgtgggtgc ggttacagcg gaggaccgtg agctggtcgc gggttgccgc ctcgcgttgg
     8881 aaaggctggg tgatccgttc acggacgatc tccctaccgg agcaattcat gccgactatt
     8941 ttcccgacaa cgttctgttc gagcacggcc aggtgtcggg cgttatcgac gtctatttcg
     9001 gctgtgatgg accgttcgcc tacgatctgg cgattgctct gacagcttgg ggcttcgacg
     9061 atgagggctg cgagattgcg ggtcagatgg aagcgttccg gcgcggctat gagtcggtac
     9121 gactgctgac ctgggccgag gaaagggcgt tgcttgtgct gggcgagacc gcggcgctac
     9181 gcttcaccct cacgcggctg cacgaccgga tttttcacga tccgaccaaa ctggtgacgc
     9241 ccaaggaccc ggccgccttc ttccgccgca tggcttactg gcgggtggcc gaaacggtct
     9301 aggccgcctc gctgcgacga agccagtcga tgaccacgtc gtgggcggcg ccgtcaaggt
     9361 aaccttcctc attggccgcc agaggtgcgc ctcgaccagc gaaaccaaca ggggcgacct
     9421 cgatcgaccg gaacccgaag cggtctcgga aggcccggtc gaaccttgcg cgcgatccga
     9481 agccgtagcg ttccgcgatg ccctggatgt tggcgagact ccccccgccc gaaacccgca
     9541 agtctgcgta ggccttgtcg agtcgccggt gcaggacata ggtctgcaca cctcccatgt
     9601 cgccgaacgc gcggtaaaga gcgctgcgtg aaacgccgat ctggagcgcg acctcattga
     9661 cgttcaaggt ggggtccgag gcgcgggcgt cgaacacctg catggcccgc tggcgaatgg
     9721 tgcgatgaat gctctccatc tgcatcgggg cgatcggccc ggcgcggccg aggaaccgct
     9781 cggagatgat gaaggtcgcc tcgatggcgg cgacgccctc ctcttcggtg agatcgcctg
     9841 cgacatcggc cagcgtcttg aggtgcgagg cgaccagtcg cccgccgggg cttgccgacg
     9901 gaagaaccag accgtgcacc ttccggccaa gcagccagga cggcactgag gtccgggggg
     9961 tcacgaggtt tatgacgtcg atagtctcga cccgggaggc tgagggccgg gagagatcac
    10021 ggaactgcac cgatcccgct tccgctgaaa tggcccggcc atcgcagtcg cccaaagtgt
    10081 cgctcagatt gactataatg ctgatctgat cgaggcccga acgacggatt tcggccgcgc
    10141 cgcgcgccag cgtctggggc acggaccggc cccgggcgaa tgaggtggcg ccgaaccgcc
    10201 aagccgttgt ctgactgcga aacccggtgc gtccgctgtc ggctacaccg gagacttcgt
    10261 agaaatcagc gattgtcgat cgatagctct cgtaggcgtc aggctgcctt tccgacgaaa
    10321 tcgcccaagc gacgctgagc gcccggtcgg gtctgattga actcactggc actggttacc
    10381 gccgactacg ccccacgcga ccgtacatcg gtatctgaca ttgtatgatt tgtcgaaaga
    10441 tgtattcgcc cagaccttta gctcatgcgg ctagcggcgg aagctccgca cggcttcgag
    10501 ggccgcctcg tgtgtgccgt caatgagtgt caggcgttcc gggcgatcac cgagctcgcg
    10561 gatgacgtgg ggcgggtcgg gatcgaagcc aagcacgggt gcaacatctt tgccaaattt
    10621 ggcggcgtgc gccgtcgaca gggcgatcat cggcccgccg gtcggctgac gacgcgcggc
    10681 ggcgagggcc acggcggtgt gcggacatac cacccggccc caggtttcat acgcctgggc
    10741 tatttccgag cgggtcgtcg cctcgtcgat ggagacggcg gagacctcgg cgcggatggc
    10801 caacaggagg tcggcgggca gggcggtctg gccgtcgcga gcgaaggttt cgaacatggc
    10861 gcgggttgct tcagcgtcgc ggcctgaccc ctcgaacacc agccgctcga agtttgacg
//
LOCUS       DASVTM010000014        10182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_320515,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000014 DASVTM010000000
VERSION     DASVTM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320515"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VF633_00320"
     CDS             <1..686
                     /locus_tag="VF633_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX8469531.1"
                     /translation="PGETTSVAIGQGAITVNALQLAVYTSRLANGKKAIQPRLIKSVG
                     GVERPSGALAPDLPFTPAHMEIVRQGMIAVANDRSGTAYRQSQLGLGDIQMAGKTGTA
                     QTRNYGSGTRKSNVWALKDHNLFIAFAPIDAPRYAVAVIVEHGGKGGATAGAPRAREV
                     MRTVLLKDPEMRDRMTRPPPPEPIGPMTEEEANFGAAPEPEIVEPATTAAARQTAPTS
                     GPRPYLAPR"
     gene            683..1840
                     /gene="rodA"
                     /locus_tag="VF633_00325"
     CDS             683..1840
                     /gene="rodA"
                     /locus_tag="VF633_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX8469532.1"
                     /translation="MTASALTRPGERDRISSKFAEIDWRFVALLCVVASVGGAMLFSV
                     AGGSWEPWAAKHLIRFGALVVLMLALSMVNLRIWFGASYIVYGLALILLMAVEVPGLG
                     VTVMGATRWLNLGFTRLQPSEIMKIGVVLALARWYHGASAQDARLSWKLIFPVAIIGV
                     PFILVAHQPDLGTAMLIGLTGAAMMFMAGLSWKIMATVGAAAAVLVPPYIMFGMHDYQ
                     RNRVLTFLNPEGDPSGNGYHIVQSKIALGSGGLLGKGFGLGSQSQLEFLPEKQTDFIF
                     AAVSEEFGFVGSITLLLCYAGIILIALRIASLSHSHFGRMASAGVTSTFALYVLINGA
                     MVMGLAPVVGVPMPLLSYGGTVMLTVMIGFGLVLATRVHRYAELPKGHGLI"
     gene            1965..3008
                     /locus_tag="VF633_00330"
     CDS             1965..3008
                     /locus_tag="VF633_00330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8469533.1"
                     /translation="MTDEREVLTRIDGAVGRITLNRPKAIHALNAGMCEAMIEALAEW
                     RDDDEVVSVLIDHAGERGFCAGGDIRMIAESGAGDASEAKAFFKTEYRLNHLMFGYSK
                     PITAIVDGIVMGGGVGISEPAAVRIATERTTYAMPETGIGLFPDVGGGWFLSRLPGQT
                     GVWLALTGARLKAADTVALGIHTHFVPSQNIMALKADLIGGVDAPEAVARHRSADPGP
                     ATIDCEAIARLFAFDSVEAIFAALEADGSEWASAQLATLKTKSPQSLKVSLRQVRTGA
                     TLTDFADNMAMEYRLGGRMAQTHDFQEGVRAVVVDKDNAPRWSPDTLEGVSDAAVDAL
                     FAPLPSDEEWTPL"
     gene            complement(3018..4238)
                     /gene="chrA"
                     /locus_tag="VF633_00335"
     CDS             complement(3018..4238)
                     /gene="chrA"
                     /locus_tag="VF633_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX8469534.1"
                     /translation="MTTAYTEPTATEPSPPSLTYSALCLRFLKFGLLAFGGPVAQIAM
                     IRRDLVDQERWISSAHFNRLLAVLQVLPGPEAHELCVHLGMRARGRLGGLIAGLGFML
                     PGFLIMLVIGWTYVSLAPLMGLLAGAFFGVQSAVIAVIVRAVHKIGSHILEDVWLWCV
                     AAAGFVATLAGVSFWVVLVAGGAAYGLLKSGRRLMAGIVTAFAVLAAMGMVWSQITTI
                     GLHGAALVPAATGTLAMFWTGLKAGLLTFGGAYTAIPYVRADTVGRGLMSDGSFLDGI
                     AFAGVIPAPLVIFCTFVGFVVGGWTGALAMTVGVFLPAFAFSMIFYERLESVVADARL
                     HHVLAGVASAVVGIIAATSLQLGWATLQRVPSVPAALAIFVAAFAAVWFWKSRYAIPV
                     VLALGALAGWVLFR"
     gene            complement(4615..5211)
                     /locus_tag="VF633_00340"
     CDS             complement(4615..5211)
                     /locus_tag="VF633_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8469535.1"
                     /translation="MPLLVGFNAEEALSLTDVSGVTAANFAEKLTARWGPLPPALLAP
                     YPFTTDDEARTARADFERDLRFGWDIRAWARLQAAHGLSQVHAYAFTRRPPYAEGSVQ
                     QHWGAGHFAELWYMFDHLDQQPWRWTETDRKLAGAMADYWAAFARHGDPNGPGRPAWP
                     AFVAGDERVLVLDELIESGVLPNNAALDVFDAVYAPLR"
     gene            complement(5399..6187)
                     /locus_tag="VF633_00345"
     CDS             complement(5399..6187)
                     /locus_tag="VF633_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8469536.1"
                     /translation="MLHHSRTALVRSLIFLSFAVVLTVGGAGYVHAQDVVTASGRLKG
                     DSDAGIVRYLGVPYARPPVGDLRWRDPQPVGTWTDVRDASRFAPACPQTGVSMPGEPP
                     SSTSEDCLYLNLWTPADAGPNSQPHPVIVWIPGGGLTNGSTSIPLYDGAALARRGVIV
                     VTVAYRLGVLGFLAHPELTEESGAGGSGNYGLMDQIAALEWVQRNIAAFGGDPERVTV
                     AGQSAGATSVSILMASPRAKVSSSARSGRAAGCSSRWRWRPATF"
     gene            complement(6430..6783)
                     /locus_tag="VF633_00350"
     CDS             complement(6430..6783)
                     /locus_tag="VF633_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469537.1"
                     /translation="MSPELACKLLAFSFPLGALGHFWWVGRHGVFYHGPAPAWAVWFW
                     YGLCAMDFVVCWLLLTQPRPGLVLAVVVMAVSLTVNWTCFPTFEHGFNGVLVGLTAFG
                     LLVFCSAPWLWRSLD"
     gene            complement(6780..8204)
                     /locus_tag="VF633_00355"
     CDS             complement(6780..8204)
                     /locus_tag="VF633_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8469538.1"
                     /translation="MSQLLEDFLGHVAARPDVPALIDGRGRVTSYAGLAAMSAERALA
                     FRRAGVGRGDVILVARGVSPELYASLLALFRLGAVAMFPEPAAGLAGLALAAEAARPR
                     AMIAAELGRAVRLLSPTLRRLPLLSESAPRPLGDGPLETISDDEPALITFTSGSTGRP
                     KGIVRSTGFLRLQHDLLERIRQTTPDDVDLISLPVFILSNLAAGATSVLPPGKITRPG
                     RLNPELMRHQIAAFGVNRVVAPPAVCARLIEGVDKPLTLDAIFTGGGPVFPNLLKGLH
                     AAAPRAAIHAVYGSTEAEPIAHIEYGDISEADWEAMSNGAGLLAGRPIPEASIAIQND
                     EITVAGPHVNRGYLDPRDDASTKFVEDGVLRHRTGDAGRLDDQGRLWLLGRHEAAARG
                     LYPFAVETAALSWSGVRQAALLADRTPALLVVAGAGLDKTALTDQAAALGEIECKVVT
                     AIPLDRRHNSKIDYAALRSRIDRS"
     gene            complement(8201..9037)
                     /locus_tag="VF633_00360"
     CDS             complement(8201..9037)
                     /locus_tag="VF633_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469539.1"
                     /translation="MSEDRIEIAGASLRIWRDAPSWDGRRTAALGDFACIDAGSGVQL
                     LAQAQRQLGAEGIEALVGPMNGDTWSTHRLVVESDGRPPFLMEPADPPHYPVAFEQAG
                     WSVVGRYASAEGPLPDTPSPAASPPGIALSSFDLHQAEAELADLHRVSLAAFTRGFLY
                     RPLSQEAFIAQYQPVLSRLDPELVVMARDDVGNLQGFLFAIPDLVDPSPERAVILKTY
                     ASLQPGLGSSLARRFYAQAAARGYRRVVHALMHDDNLSARHSARLGATVFRRYALWGS
                     VL"
     gene            complement(9034..>10182)
                     /locus_tag="VF633_00365"
     CDS             complement(9034..>10182)
                     /locus_tag="VF633_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469540.1"
                     /translation="LAGGVFVIARARPSLGEGLHGVKRFSYGELLFAVSVALLFWLKD
                     GHYSLAHRGLQTEPQLILYILPLLILTLCDAASALIGVNYGRLQFPVEEGVKSVEGVV
                     TFILTAWIAALVSLLLFTDLGRGDVIVLALIAALFGSLFEAASWRGLDNLFIPIGLYF
                     ILSNLLPRGTTELLITTFAFAGGSALLMGLGVRLGLTRHIVASGMSLLFCIAIFAGPM
                     AILTPCATFAAYYVVWRDGARRDVSHDALNMVITVLTVALSVFVLSAVLKVDTIFVFN
                     LAFACLATGILVRFSDRGAAWLAGGIACICAVALVRTLAVPVWRPGDLIFAACAVLLV
                     GVSAGLFKLVRDRWPDRPWIKLGTISFLIGLAGLPLAPTAWAPLGLSG"
BASE COUNT         1910 a         3234 c         3483 g         1555 t
ORIGIN      
        1 atccgggcga gacgacttca gtagcgatcg ggcaaggcgc gattaccgtc aacgcgctgc
       61 agctggccgt ctatacgtcc cggttggcga acggaaagaa agcgatccag ccgcgcttga
      121 tcaagtcggt aggcggcgtg gagcgaccca gtggagcact cgcaccagat ctgccgttca
      181 ccccggcgca tatggagatc gtccgccaag gcatgatcgc cgtggctaac gaccgttccg
      241 gtacggctta tcgtcagtcg cagctgggac tcggcgacat ccagatggcc ggtaagaccg
      301 gtaccgcgca gacgcgcaac tatggatcgg gaacgcggaa gtcgaacgtg tgggcgctca
      361 aggaccacaa cctgttcatc gccttcgcgc cgatcgatgc gccccgttat gcggtggctg
      421 ttatcgtcga acacggcggc aagggtgggg cgacggcggg cgctccacgt gcccgcgagg
      481 tgatgcgaac ggttctgctc aaggatccgg agatgcgcga tcgcatgact cgcccgccgc
      541 ctccagaacc gatcggtccg atgaccgaag aagaagccaa tttcggtgcg gctcctgaac
      601 cagagatcgt tgagccggcg acgacggctg cagcgcgtca aaccgctccg acatccggcc
      661 cgcgacccta tctggcgcct cgatgaccgc ttccgccctg acccgtcccg gcgagcgcga
      721 ccggatttcg agcaaattcg ccgagatcga ctggcgcttt gtggcactgc tgtgcgtcgt
      781 cgccagtgtg ggcggcgcaa tgctgttctc ggtggccggg ggctcgtggg agccttgggc
      841 cgccaaacac ctgatccggt tcggcgcgct cgttgtgctg atgctggcgc tgtcgatggt
      901 caatctgagg atctggttcg gggcgtccta tatcgtctac gggctggcgc tgatcctgct
      961 gatggcggtc gaggtgccgg gactgggcgt caccgtcatg ggcgcgacgc ggtggctgaa
     1021 tcttggcttc acccgtctac agccctcgga gatcatgaag atcggcgtcg tcctggcgct
     1081 ggcgcgctgg tatcacgggg cttcggcgca ggatgcacgg ctgagctgga agctgatctt
     1141 ccctgtcgcc atcatcggcg tgccgttcat attggtcgcc caccagccgg acctcggcac
     1201 cgccatgctg atcggtctga cgggcgcggc gatgatgttc atggccgggt tgagctggaa
     1261 gatcatggcc acagttggcg ctgcggccgc ggtccttgtg ccgccctaca tcatgttcgg
     1321 gatgcacgat taccagcgaa accgcgtcct gaccttcctt aatccggaag gcgatccaag
     1381 cggcaacggc tatcacatcg tccagtccaa gatcgcgctc ggctccggcg gccttctggg
     1441 caaggggttc ggtctcggta gccagagcca gcttgaattc ctgcccgaga agcagaccga
     1501 tttcatcttc gccgctgtgt ccgaggagtt cggcttcgtc ggctcgatca ccctgctgct
     1561 ttgctatgcc gggatcatcc tgatcgcact gcggattgca tcgttgtcgc acagccattt
     1621 tggccggatg gcctcggcag gggtgacctc gaccttcgcg ctctatgtgc tgatcaatgg
     1681 ggcgatggtc atgggtctcg cgccggtcgt gggcgtaccg atgccgctgc tgtcgtacgg
     1741 cggcacggtg atgctgacgg tgatgatcgg cttcggtctg gtgctggcga cgcgcgtcca
     1801 ccgttatgcc gaattgccca agggccacgg gctgatctga tgcgcctggc ggacggggcg
     1861 agagcccgcg tcttgcacca ccaccctttc ggcgacgcat cgccacgccc tgcgagcctc
     1921 atgccctctc ccgccgggac agggagtcgg atatgagcaa gatcatgacc gacgaacgcg
     1981 aagtcctgac ccgtatcgat ggcgccgtgg gacggatcac gctgaaccga cccaaggcga
     2041 tccatgcgct gaacgcgggg atgtgcgagg cgatgatcga ggccttggcg gagtggcgcg
     2101 acgacgacga agtggtctcg gtgctgatcg accatgcggg cgagcgcggg ttctgcgccg
     2161 gcggcgacat ccgcatgatc gcggagagcg gagcagggga tgcgtcggag gcaaaggcct
     2221 tcttcaagac cgagtaccgg ctgaaccatc tgatgttcgg atattccaag ccgatcactg
     2281 ccatcgtcga cgggatcgtc atgggcgggg gcgtgggtat ctcggagccg gcggcggtac
     2341 ggatcgcgac cgaacggacc acctacgcca tgccggagac cgggatcggc ctgtttcccg
     2401 acgtcggtgg cggctggttt ctgtcgcgat tgccggggca gacgggcgtg tggctggcgc
     2461 tgaccggggc gcggctgaag gcggcggata cggtggcgct ggggatccat acgcatttcg
     2521 tgccgtcgca aaacatcatg gcgctgaagg ccgatctgat cggcggggtg gatgcgccag
     2581 aggcggtggc gaggcatcgc tcggcggatc cgggacccgc gacgatcgat tgcgaggcca
     2641 tcgcccggct gttcgccttc gacagcgtgg aggcgatatt cgccgcactg gaggccgacg
     2701 gatcggaatg ggcttcggct caattggcga cgctgaagac caagtcgccg cagtcgctca
     2761 aggtgtcgct gagacaggtg cggaccggcg ccaccctgac cgacttcgcc gacaacatgg
     2821 ccatggaata ccggctgggc gggcggatgg cgcagacgca tgacttccag gaaggcgtcc
     2881 gcgccgtggt cgtggacaag gacaacgccc cccgatggtc gccggatacg cttgagggcg
     2941 tatcggacgc agcggtggat gccttgttcg caccgctgcc ttccgatgag gaatggacgc
     3001 cgctctaggc gacgccgtca gcggaacagc acccaaccgg ccagggcgcc cagcgcgaga
     3061 acgacgggga tggcgtagcg gcttttccag aaccacaccg ctgcgaatgc cgccacgaag
     3121 atcgccagcg ctgccgggac tgaaggcacc cgctgcagtg tcgcccagcc gagctggagg
     3181 ctggtcgcgg cgatgatgcc gacgacggct gaggcgacgc ccgcaagaac gtggtgcagc
     3241 cgggcgtccg cgaccacgct ttccagccgc tcgtagaaga tcatcgaaaa ggcgaaggcg
     3301 ggcaggaata cgcctacggt catcgccagc gccccggtcc agccgcccac gacgaagccg
     3361 acgaaggtgc agaagatcac gagcggcgcg gggatcacgc cggcgaaggc gatgccgtcg
     3421 aggaaggacc cgtcgctcat caggccccgg cctaccgtgt ctgcgcggac ataggggatg
     3481 gccgtatagg cgccgccaaa ggtcagcagg ccggctttca gtcccgtcca gaacatggcc
     3541 aaggttcccg tggctgcggg cacgagagcc gcaccgtgca acccgattgt ggtgatctgg
     3601 ctccagacca tacccatcgc ggccagcaca gcgaacgcag tcactatccc ggccatcaag
     3661 cgccgaccgc ttttcagcaa gccgtaggcc gcgccgcctg cgaccagcac tacccagaag
     3721 ctgacacccg ccaatgtggc gacgaagcct gccgccgcga cgcaccacag ccagacgtct
     3781 tcgaggatgt gggagccgat cttgtgcacg gccctgacga tcacggcgat gacggccgac
     3841 tgaacgccga agaaggcccc ggccagcagt cccataagag gcgcgagcga cacataggtc
     3901 cagccgatca ccagcatgat aaggaacccg ggcagcatga agccgagacc ggcgatcaga
     3961 cccccaaggc gacctctggc cctcataccc agatggacgc aaagctcatg agcttccggc
     4021 ccgggaagaa cttgcagcac cgcaagcagc cggttgaagt gggcgcttga gatccagcgc
     4081 tcctgatcga caaggtcacg tcggatcatc gcgatctgag cgacaggccc cccgaacgcc
     4141 agcaacccga acttcaggaa gcgcaggcac agggcggagt aggtgaggga gggcgggctc
     4201 ggttcggtag ccgttggctc agtatatgcg gtggtcacag tcattgctcc ggcctgcgcc
     4261 tcaccggcac aggaaaagga gcggagcttg gacggaaccc gtctgaccgg gcgtccgcgt
     4321 gtgcccgacg ccgaagcgta gccgggtcag gatcgtctgc caagtgggat ctggtgatgg
     4381 tgagcctggc ctgacgctgt ccccgatgca ttggaacgat agcccgtaac tgatgcaaac
     4441 ccggaccttc cgaacttccg ccaagggcgg aaagcggatg tcgacaaggc gggctcggaa
     4501 gtcctgagtc tgtcattcga ggcaccattc cggaaaccgc ctgacccccc agccgccatt
     4561 ggggatcaga tcggtgtgat caaaagcata cccgtttgca gaaaacggtg cccgtcagcg
     4621 gaggggtgcg tagaccgcgt cgaacacgtc gagcgccgcg ttgttcggca gcactcccga
     4681 ctcgatcagc tcgtcgagga caagcacacg ctcgtcgccc gccacgaacg cgggccaggc
     4741 cgggcggccg gggccgttcg ggtcgccgtg tctcgcgaag gccgcccaat agtcggccat
     4801 ggcgccggcc agtttccggt cggtctccgt ccagcgccac ggctgctgat cgaggtgatc
     4861 gaacatgtac cagagctcgg cgaaatgccc cgcgccccag tgttgctgaa ctgagccctc
     4921 ggcataaggc ggccgccggg tgaaggcgta ggcgtggacc tggctgagcc catgggccgc
     4981 ctgaagccgc gcccaggcgc ggatgtccca gccgaagcga aggtcgcgtt cgaaatccgc
     5041 gcgcgccgtg cgcgcctcgt cgtcggtggt gaaaggatag ggggcgagca gggcgggcgg
     5101 cagcggcccc caccgcgccg tcagcttctc cgcgaaattc gccgcggtga cgccggacac
     5161 gtccgtcagg gacagcgcct cctccgcgtt gaaaccgacc aacagaggca cgtcgttctg
     5221 gcgcccggcg gtgaaaacat cccacggcgt ctccggtagt acgcgcggct cgatgacggg
     5281 atgaacgatc ccttcggacg ccggcccagg aaccgatcag cgggcaggcc gcgaagctgc
     5341 gccagggtcg gcgctcccaa ccctgtggcg tacgcagcgc cgtcaacctc ggcgtttttc
     5401 agaaggtagc ggggcgccag cgccagcggc tcgaacagcc cgccgctctg cccgatcgcg
     5461 cgctggaaga gacctttgcc cggggggacg ccatgaggat actgaccgag gtcgcgcccg
     5521 cggactggcc agcgaccgtg acccgctcgg ggtcgccgcc gaaggccgcg atgtttcgct
     5581 ggacccactc cagtgcagcg atctggtcca tcagaccgta gttcccggag ccgccggccc
     5641 cggactcctc cgtcagctcc ggatgagcaa ggaagcccag cacgccgaga cgataggcga
     5701 cggtcacgac gatcacccct cgacgcgcga gggcggcccc gtcgtacagc gggattgagg
     5761 ttgagccgtt ggtgagtccc ccgccgggga tccagacgat gacgggatgc ggctgactgt
     5821 tcggaccggc gtccgcaggg gtccatagat tgagatagag gcaatcttca ctcgtcgacg
     5881 agggcggctc gccgggcatg gatacgccag tctgcgggca ggccggtgcg aagcggctgg
     5941 cgtcgcggac gtccgtccat gtccccaccg gctgagggtc gcgccagcga agatcgccaa
     6001 cgggcggccg tgcataggga accccgagat accgcacgat cccggcgtcg ctgtcgccct
     6061 taagccgtcc gctggcggtg acgacgtcct gcgcatgaac gtagcctgcc ccgcccaccg
     6121 tcaggaccac ggcgaaggac aggaagatga gggatctgac gagagcggtt ctggaatggt
     6181 gaagcatagc cttaagaggc cgcgacggag ctcagcggat gcagtaagtc gcaaaaatgg
     6241 tcacgcgctt tccgcgagtc caatgcgcag cgaggttgag gacactaccg gccggcgcct
     6301 gctgagatgg gcgtacgtct gcgtagccgc gccgccgtgg acgaaaccga gattgattgc
     6361 ccggtaatgc ggatgagcgc cggaggggac aagacgcggg tcccgtagcg gtcatcgcca
     6421 aacatttcct cagtctagcg atctccaaag ccagggcgcg ctgcagaaga ccagcaggcc
     6481 gaaagcggtc aggccgacca gaacgccgtt gaagccgtgt tcgaatgtcg gaaagcaggt
     6541 ccagtttacc gtcaggctga cggccataac cacgacggcg agaacgaggc ctggacgcgg
     6601 ctgggtgagc aacaaccagc acaccacgaa gtccatcgcg cagagcccat accagaacca
     6661 cacggcccag gccggagcgg ggccgtggta gaacaccccg tgccgaccga cccaccagaa
     6721 atggccgaga gcgcccagcg gaaagctgaa ggccagcagt ttgcaggcga gttcggggct
     6781 cacgagcggt ctatccggga ccgcagtgcg gcgtagtcga tctttgagtt atgccgcctg
     6841 tccagaggaa tggcggtgac cactttgcat tcaatctcgc ccagggcggc ggcttggtcg
     6901 gtcagagcgg tcttgtcgag cccggcgccg gcgacgacca gaagggcagg ggtgcggtcg
     6961 gccaggagcg cggcctgacg cactccactc cagctcaggg cggctgtttc gacagcgaac
     7021 ggatagaggc cccgcgccgc agcctcgtgt cggccgagca gccaaagccg cccttgatcg
     7081 tcgagccggc ccgcgtcgcc agtccggtgt cgcaggacgc cgtcctcgac gaactttgtc
     7141 gaggcgtcat cgcgtggatc aaggtagccc cgattgacgt gcggtccggc gacggtgatc
     7201 tcgtcgttct gaatcgctat ggaggcctcg gggatcggac gtccggccag aagccccgcg
     7261 ccgttcgaca tggcttccca gtcggcttcg gatatatcgc catactcgat gtgcgcgatg
     7321 ggctcggcct cggtcgatcc atagacggca tgaatggcgg ctcggggcgc cgcagcgtgg
     7381 agccccttca gcagattggg aaacaccggc ccgccgccgg tgaagattgc atcgagcgtt
     7441 agcggcttgt ccacgccttc gatcagccgg gcgcagacgg cggggggagc gacgaccctg
     7501 ttgacgccga acgccgcgat ctggtgtcgc atcagctcgg ggttgagccg gccggggcgg
     7561 gtgatctttc ccggcggcag gacgcttgtc gcgcccgccg cgaggttgga caggatgaag
     7621 acaggcaggg agatcagatc gacatcgtcg ggcgtcgtct gcctgatcct ctcaagcagg
     7681 tcgtgctgta gacggaggaa cccggtcgag cgaacgatgc ccttgggtcg gcccgtcgat
     7741 cccgaggtga aggtgatcaa ggcgggctcg tcgtcgctga tggtttccag tggaccatcc
     7801 cccagcggtc tgggggcgct ctctgacagc agcggcaacc tacgcagggt cggggacaac
     7861 agccggacag cccgccccag ttcggccgcg atcatagccc gggggcgcgc cgcctcggcc
     7921 gcgagagcca gaccagccag accggcggct ggctcaggaa acatggcgac cgcgccgagc
     7981 cggaacagag ccagaaggct ggcgtacagt tcgggcgaaa cgccgcgcgc gaccagtatg
     8041 acgtcgcctc gaccaacacc ggcgcgtcgg aacgctaagg ctcgctccgc cgacatggcg
     8101 gccagccctg cataggaggt tacgcggccg cgcccgtcga tcagggcagg gacatcggga
     8161 cgagccgcga cgtgtccgag aaaatcctca agcagctggc tcacaggacg ctaccccaca
     8221 gggcgtatcg gcggaacacc gtcgccccga gcctggcgct gtgtcgggca gacaggttgt
     8281 cgtcgtgcat cagggcgtga acgacccggc ggtaaccgcg cgcggctgcc tgcgcataga
     8341 accgccgcgc gagactggaa cccagtccgg gttgcagcga ggcgtaggtc ttgaggatca
     8401 cggcccgctc gggcgatgga tcgaccagat cgggtatggc gaacaggaaa ccctgcaggt
     8461 ttccgacgtc atcgcgcgcc atgaccacca gttccggatc cagccggctg agtacgggct
     8521 gatactgcgc gatgaacgct tcctgcgaca aggggcggta caggaagcct cgcgtgaagg
     8581 cggccagcga aactcggtgc aagtcggcaa gctcggcttc ggcctgatgc aggtcaaacg
     8641 acgagagtgc gatgcccgga ggcgaagcgg cagggctggg cgtgtccggc aacggcccct
     8701 ccgccgaggc gtatcggccg accaccgacc acccggcctg ttcaaaggcg acgggataat
     8761 gcggcgggtc ggccggttcc atcaggaagg gagggcggcc gtcgctctcg accaccagtc
     8821 ggtgcgtgga ccaggtgtcg ccgttcatgg gaccgaccag ggcttcgatc ccttcggccc
     8881 ccagctgtct ttgggcttgg gccagtaact gaactcccga gccggcgtcg atacaggcga
     8941 aatcccctag agccgccgtc cgcctgccgt cccacgacgg agcgtcgcgc cagatgcgca
     9001 gggaggcgcc cgcgatctcg atccggtcct cgctcatccc gacaatccca aaggggccca
     9061 cgcggtcggc gcgaggggaa gtccggccag tccgatgagg aagctgatcg tacccagctt
     9121 gatccagggg cgatcgggcc accgatccct caccagtttg aacagccctg cggaaactcc
     9181 gaccagcaaa accgcacagg cggcgaagat caggtctccc ggtcgccaga cgggcacggc
     9241 cagcgtccgc acgagcgcga cggcgcagat gcaggctatg ccccccgcga gccacgccgc
     9301 gccacggtcc gagaagcgta cgaggatgcc ggtggcgagg caggcgaagg cgagattgaa
     9361 gacgaagatc gtatcgacct tgagcacggc gctgagcacg aacacgctga gcgcgaccgt
     9421 caggacggtg atgaccatgt tcagggcgtc gtgcgaaacg tctcgccgcg ctccgtcacg
     9481 ccagacgaca tagtaggcgg cgaaggtggc gcagggcgtc aggatcgcca ttggtccggc
     9541 gaagatcgcg atgcagaaca gcaggctcat gccactggcg acgatgtggc gcgtgagccc
     9601 cagccgcact cccagcccca tgagcagagc cgatccgccg gcgaaggcga aggtggtgat
     9661 cagcaattcc gttgtgccgc gcggcagcag gttggaaagg atgaaataca ggccgatcgg
     9721 gatgaacaga ttgtccaagc cgcgccagct tgccgcttcg aacagcgatc cgaacagcgc
     9781 tgcgatcagg gccagaacga tgacgtcgcc tcgtcccaga tcggtaaaca gcagcaggga
     9841 gaccagagca gcgatccacg cggtcaggat gaaggtcacc acgccctcga cgctcttgac
     9901 gccttcctcg accgggaact gcagccggcc gtagttgacg ccgatcaggg cgctggcggc
     9961 gtcgcacagc gtcaggatca gcaagggcag gatgtagagg atcaactgcg gctcggtctg
    10021 cagtccacgg tgagcgagcg aatagtggcc gtccttgagc cagaagagca gggcgaccga
    10081 gaccgcgaac aagagctcgc cataggagaa tcgcttgacg ccgtgcagac cctcgccaag
    10141 cgaagggcgg gcccgggcga tgacgaaaac accgccggcc ag
//
LOCUS       DASVTM010000015        11959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_161078,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000015 DASVTM010000000
VERSION     DASVTM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161078"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VF633_00370"
     CDS             complement(<1..568)
                     /locus_tag="VF633_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8469541.1"
                     /translation="MFDLTGKTALVTGATGGIGGAIARALYAQGATVVLSGTREAVLA
                     DLAKELGERAHFATANLSDPASVDALVGVAEEVAGAGLDILVANAGITKDGLLMRMKD
                     EDFQSVLTVNLESYFRLSRAAMKGMMKRRSGRIIGITSVVGVTGNGGQTNYAASKAGM
                     IGFSKSLAQEVGSRGITVNCIAPGFITSP"
     gene            complement(583..1530)
                     /gene="fabD"
                     /locus_tag="VF633_00375"
     CDS             complement(583..1530)
                     /gene="fabD"
                     /locus_tag="VF633_00375"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX8469542.1"
                     /translation="MTLALLFPGQGSQSVGMGAALADAFQSARDVYAEIDQALGQNLS
                     QLMREGPEDQLTLTENAQPALMAVSVAAARVLKVEFGVDMTAASYVAGHSLGEYSALC
                     AVGAISLADTARLLKLRGQAMQRAVPVGQGAMASLIGPRTDLALAEEAAKVGSEVGVC
                     VVANDNNAGNIVISGDKAAVDRAIEKAKELGARAIPLNVSAPFHCPLMQPAADEMATA
                     LATATILAPAVPIVANVTASPVTDPEVVRRLLVEQVTGRVRWRESMIWMVGEGGVTRV
                     AEIGSGKVLTGMAKRIAPEAEAVALNTPEDLEAFAKSIA"
     gene            1781..2167
                     /gene="rpsF"
                     /locus_tag="VF633_00380"
     CDS             1781..2167
                     /gene="rpsF"
                     /locus_tag="VF633_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX8469543.1"
                     /translation="MALYEHTVMTRQDISAQQAEALNDTIKDLIVAGGGTVAKIEYWG
                     LRNLTYRVKKNRKAHYSLLAIDTPPAAMAEVERQLGINEDVLRWLTVKVEELDLELSP
                     LLARRERERERDRDRPVRNDDVVVEA"
     gene            2179..2463
                     /gene="rpsR"
                     /locus_tag="VF633_00385"
     CDS             2179..2463
                     /gene="rpsR"
                     /locus_tag="VF633_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX8469544.1"
                     /translation="MTDTTAPRNVPGTPAGSGGSGRRPFNRRRKVCPFSGEGAPKIDY
                     KDVKLLQRYISERGKIVPSRITAVSQIKQRELAKAIKRARYLALLPYVVK"
     gene            2465..3049
                     /gene="rplI"
                     /locus_tag="VF633_00390"
     CDS             2465..3049
                     /gene="rplI"
                     /locus_tag="VF633_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX8469545.1"
                     /translation="MKVVLLERVDNLGAIGDVVTVKDGFARNFLLPRDKALRATSKNL
                     EKFELDRVAIEARNDKNKEASQKIADKIDGESYVMIRQAGETGQLYGSVAGRDVSEAI
                     QAEGGKVERSQIVLNTPIKSLGLHEVLVRLHPEVSATVKINIARSADEAERQAKGEDV
                     IKSAYEEDRNAAAEQASDMRAGGAGQQDGLGSEA"
     gene            3421..5844
                     /locus_tag="VF633_00395"
     CDS             3421..5844
                     /locus_tag="VF633_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469546.1"
                     /translation="MLACPLAASAQTTPAEPQDESAVVEDIVVTGTRAVGRSRLDSIA
                     PVDVISAETLSRQGTGTELAAALAATAPSINFPRPAISDGSDHVRPATLRGLAPDQTL
                     VLINGQRGHVGALINVNGALGRGSTAFDLNTIPSVSLGTVEVLREGASAQYGADAIAG
                     VINLRLRQADHGGGITANYGIYNTDFTTARGDHSETDGEQTSVAGWIGLPLFGDGFLT
                     LSAELQKREPTNRSDYALPSAVNGGSANTVIGRFGDPYTEGYTGYFNAGKPLSNGWDA
                     YAFGGYQHRYSEAAATARQFNNANNVPAIYPNGFLPIIAASIDDYNIYSGVKGAALGW
                     DWDLSVGYGRNELEYRVLDSLNASYGAASKTTFDAGSLSYGQLTVGIDAVKQIEANLY
                     EPLNVALGVEYRREDFSVSEGEPASYNKGPVTTGGSGSQGFPGFAPSNAVDEDRSNIA
                     AYIDVEGKLSEQFSLGVAGRYEDYTDFGSQFTGKVSARYDFTPEFALRGAISTGFKSP
                     ALQQQYFSYVATNLTTTVVGGVPVTTLSQNGTYRVDDPIAVSLGSKPLEPETSTNYSL
                     GAVFRHEGFELTVDTYRIDLEDRIIYSETLGVSRPSQTAVSTAAIQALLAPYGVTGAR
                     FFLNGVDTTTSGVDVVARYRTTQDMGRFDFTLAGNFNSTEVTRTPAVPANLAIAPSGS
                     FLFDRSSRLSFEEGTPQQKIVASVDWTLGDMGATLRATSYDSVLVANNNDTLDYETGS
                     AVTVDLEGRYTFPMGISAAIGVNNLTDEYPTATLASLNGVTGSVGYPSYSPYGFNGRF
                     FYGRLSYAF"
     gene            5982..6350
                     /locus_tag="VF633_00400"
     CDS             5982..6350
                     /locus_tag="VF633_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469547.1"
                     /translation="MLKRVAAIAIAVSLIATAASAMTVQEFLTTASHIPQNPTALLRS
                     DTRRLMGEFRGAVRTVRAEQTAATTAGRRSPTCAPEKVSFSVNDILTRFNAIPAQRRS
                     QISVTQAVREWMVERYPCPA"
     gene            6506..7993
                     /locus_tag="VF633_00405"
     CDS             6506..7993
                     /locus_tag="VF633_00405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX8469548.1"
                     /translation="MNAFTPFPDTVTVPSLPHNLEAEQALLGALMFDNAIFERLSDRL
                     RGSHFFEPFHQRLYDAIEDHIRQGMLAEPTILMERFKQDPAFAEFGGLRYLADLMDRA
                     PPGANAPDYARVVYDLALRRDLIRIGGDMIKEAPDPETTAIDQIEQAELTLYSLAESG
                     APSSGFVSFSTALAGAVQMAGEAYQREGKLAGLATHLDDLDSKLGGMHPSDLLILAGR
                     PSMGKTALATNIAFNVARNYRWEPTPDGRKTVSGGVVAFFSLEMSAEQLAMRILADAS
                     GVSSDKLRKGEIDASDFGKVRDAAIEIGESPLYIDATGGLAISKLAARARRLKRMEQG
                     LDLIIVDYLQLITVGEGNGNKNRVQEVSEITGGLKALAKELNVPIIALSQLSRQVENR
                     EDKRPQLSDLRESGSIEQDADAVMFVYRESYYLGRTEPREGTPEHLQWQEDMDRLQHQ
                     AEVVIGKQRHGPIGIVKLSFDADTTRFGNLARDGRYGSSFADIPE"
     gene            8035..9078
                     /gene="alr"
                     /locus_tag="VF633_00410"
     CDS             8035..9078
                     /gene="alr"
                     /locus_tag="VF633_00410"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8469549.1"
                     /translation="MSTPARLTIDLNALARNYHTLEAVSGLPVHPVVKADGYGLGAAA
                     VAGRLMVEGARTFFVARGSEGVALRQALGPEPVIYILDGCHGDQAAMLKAADLRPVIN
                     HGQQQAAWTAAGGGACGLQIDTGMNRLGFRPEDAPEPFEGLSLVMTHLACADEPAEPM
                     NRHQRDAFTAASLKYPGVTRSFANSSGCFLGDDFRFDVVRPGICLYGGGPEGKPDSRI
                     APVATLTAEVLQTRDVPAGESVGYSRGFIADRPVRIATCATGYADGLIRATGTSGSGQ
                     VWISGQMRPILGRVSMDVLAVDITGLDVVAGDDVELFGANRLVDHAAAAAGTISYEML
                     TSVMPRVSRTYIG"
     gene            complement(9085..9693)
                     /locus_tag="VF633_00415"
     CDS             complement(9085..9693)
                     /locus_tag="VF633_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX8469550.1"
                     /translation="MTDNPPALDPSLVARVKGILLNPKVEWEVIEREFATVPTLFSRY
                     AMILAAIGPVCGLIGSVVFSHGNVIGALVAAVLGYALSLAGVFLLGLVIEALAPTFDG
                     TKDRVSAMKVAVYSATASWIGGVFSLIPALAVIGVIFSLYGLYLLYLGLTPVMKSPEQ
                     KTVAYTAVVIVAAIVIWTIIAVIVGAVFASFIGAAALATATV"
     gene            9830..11194
                     /gene="radA"
                     /locus_tag="VF633_00420"
     CDS             9830..11194
                     /gene="radA"
                     /locus_tag="VF633_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX8469551.1"
                     /translation="MARDGAIYVCQSCGAVHGKWAGQCNACNAWNVIVEESRSAPPGA
                     LKPQSASKTRGLQFESLMSETPEPPRIVTGVSEFDRVCGGGVVPGSAILLSGDPGVGK
                     STLLLDVAGRAALAGARVAYISGEEAIEQIRARAKRMGLEKAPVALASATALRDILGT
                     LKREKFDIVIIDSVQTLWSDAHEAGPGSVTQVRACAGELVRMAKAGGPAVILVGHVTK
                     DGQVAGPRVVEHMVDAVLSFEGERGYPFRILRAAKNRFGATDEIGVFEMGDAGLREVA
                     NPSALFLGEGKDRAPGAAVFAGIEGTRPVLVEIQALVAPSAYGTPRRAVIGWETGRLA
                     MVLAVLEARCGIGFGNQDVYLNVAGGLRINEPAADLAAAAALISSATGVPLPQGCVVF
                     GEISLSGEVRAVGRAEARLREAQKLGFDHVLSHPLTVKGDGVLVTGVSRLTEAVERIS
                     ENRY"
     gene            11228..11824
                     /locus_tag="VF633_00425"
     CDS             11228..11824
                     /locus_tag="VF633_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX8469552.1"
                     /translation="MTGFDAFAILVILASAAAGWVRGGTRELITLLSFVLAAFLSLVS
                     LPLTSRLGRALIDPDWAGTIFAAVAAFLIIYFGIRIFGSMLSKRAQAHPTLGGVDRFV
                     GVFVGAARSLVLLGAIHLVIVAAMPGDRTPAWLKDAALRPVSAGAARMIQIILPGIGR
                     GADIISPVVDSSVRRGFSDDGALPRPQTGNTSPPVPAP"
BASE COUNT         2183 a         4036 c         3619 g         2121 t
ORIGIN      
        1 tgggcgatgt gatgaagccc ggagcgatgc aattcaccgt gatgccccgt gagccgactt
       61 cctgggccag agacttggag aagccgatca tcccggcctt ggaggctgca tagttggtct
      121 ggccgccgtt gccggtgacc ccgacgaccg aggtgatgcc gatgatccgc cctgaacgac
      181 gtttcatcat ccccttcatc gccgcgcgcg acaggcggaa atagctttcc agattgacag
      241 tcaggacgga ctggaagtcc tcatccttca tccgcatcag gaggccgtcc ttggtgatgc
      301 ctgcgttggc taccaggata tcgagacccg cgcctgcaac ctcttccgcc acgccgacca
      361 gcgcgtcgac cgaagcggga tcggaaagat tggccgttgc gaaatgcgcg cgctcgccca
      421 gttcctttgc cagatccgcc aacaccgctt cacgggtgcc ggacaggacg acggttgcgc
      481 cttgagcata cagggcgcgg gcaatggctc ctccgatgcc gccagtggcg cctgtgacga
      541 gggcggtttt gccggtgagg tcaaacatag gtcttctcca gctcaggcga tcgacttcgc
      601 gaacgcttcc aggtcttcag gcgtattgag ggccacggct tcggcctccg gcgcgatgcg
      661 cttggccatg ccggtcagga ccttgcccga accgatctcg gcaacgcggg tcacgccgcc
      721 ttcgccaacc atccaaatca tactctcgcg ccaacgcaca cgaccggtga cctgttcgac
      781 cagcagacgg cggacgacct cgggatcggt gaccggcgag gccgtgacat tggccacgat
      841 cggcacggcg ggcgccagaa tggtcgcggt ggccagcgcc gtcgccatct cgtcggcggc
      901 gggctgcatc aaaggacagt ggaacggcgc cgagacgttc aacgggattg cccgcgcgcc
      961 cagttccttg gccttctcga tcgcccggtc aacggcggcc ttgtcgccgg agatgacgat
     1021 attgcccgcg ttgttgtcgt tggccacgac gcagacgccg acctcggacc ctacctttgc
     1081 agcttcctcc gcaagggcga gatccgtcct gggtccgatc agcgaggcca tggccccctg
     1141 ccccacaggc acggcgcgct gcatggcctg accccgcagc ttgagcagcc gcgccgtatc
     1201 cgccagcgag atcgcaccca cggcgcacag agccgaatat tcgcccagcg aatgcccggc
     1261 gacgtagctg gccgccgtca tatccacacc gaactcgacc ttcagcaccc gcgccgccgc
     1321 gaccgacacc gccatcagcg ccggctgggc attttcggtc agggtcaact ggtcctcagg
     1381 gccttcgcgc atcagctgcg acaggttctg gccgagcgcc tgatcgatct cggcatagac
     1441 gtcgcgtgcg ctctggaagg cgtcggcgag agccgcgccc atgccgaccg actggctgcc
     1501 ttgcccggga aacagaagcg cgagggtcat atatagaggt ccgctggccg ttgaatcagg
     1561 agcggagggg taggacgatc gttccgtcag ggcaagcagg gttgcatcac ccttgccgga
     1621 tcgccggttt tcccctatac gcccccgctt tccagggctt cggtcctgat gcggaacgcc
     1681 aaagggttcg ggaagtcatt ccggccgccg cttcaggagc catcgcaggc gtgacttacc
     1741 cggtccgtcg gcgccgacgc cctacaaggg agactgacga atggctctat acgagcacac
     1801 ggtcatgacg cgccaggata tcagcgcgca acaggccgaa gccctcaacg acacgatcaa
     1861 ggacctcatc gtcgccggtg gcggcaccgt ggccaagatc gaatactggg gcctgcgcaa
     1921 cctgacctat cgggtcaaga agaaccgcaa ggcacactat tccctgctcg ccatcgacac
     1981 gcctccggcc gctatggctg aagtcgagcg tcaactgggc atcaacgaag acgtcctgcg
     2041 ttggctgacc gtcaaggtcg aggaactcga cctcgaactg tcgccgctgc tggcccgccg
     2101 cgaacgtgag cgcgaacgcg accgcgatcg tcccgtccgc aacgacgacg tcgtagtcga
     2161 agcgtaagga tcagacatat gaccgataca actgctcccc gtaacgtccc gggtacgccc
     2221 gccggctccg gcggctccgg ccgtcgtccg ttcaatcgtc gtcgcaaggt ttgcccgttc
     2281 tcgggtgaag gcgcgccgaa gatcgactac aaggatgtga agctgctgca gcgttacatc
     2341 tccgaacgcg gcaagatcgt gccctcgcgc atcaccgccg tttcccagat caagcaacgc
     2401 gaactggcca aggccatcaa gcgcgcccgc tatctggccc tcctgcctta cgtggtgaaa
     2461 taagatgaag gtcgttctgc tcgaacgcgt cgataacctc ggcgccatcg gcgacgtcgt
     2521 aaccgtcaag gacggtttcg ctcgtaactt ccttctcccc cgcgacaagg cgctccgcgc
     2581 tacgtcgaag aacctggaga agttcgaact cgaccgcgtc gccatcgagg cgcgcaacga
     2641 caagaacaag gaagcttcgc aaaagatcgc cgacaagatc gatggtgaat cctacgtcat
     2701 gatccgtcag gccggcgaaa ccggccagct ttacggttcg gtcgcaggtc gtgatgtctc
     2761 cgaagccatt caggctgaag gcggcaaggt cgagcgctcg caaatcgtgc tcaacacgcc
     2821 gatcaagtcg ctgggtctcc acgaagtgct ggttcgcctg caccccgagg tttccgccac
     2881 tgtgaagatc aacattgccc gctcggccga cgaagccgaa cggcaggcca agggcgaaga
     2941 cgtcatcaag tctgcgtatg aagaagaccg caacgccgcc gccgaacaag cgagcgacat
     3001 gcgggccggc ggcgcaggcc aacaggacgg tctcggctcc gaagcctgat cgacctgact
     3061 ttggtacgat aagatcaagg gcgcagccat cggctgcgcc ctttttcgtg cccttgatgc
     3121 ggcgggcaca tgtcaaaaat gcaacaacca tggatcaatt ggttgaacca tgcttgacca
     3181 tcactgccgg cgcaatcagc ttgtgcccaa ggacggcatc gagcactttc gggcaagatt
     3241 tttgcagcgg gtgtccaata gactgaccac aggaaacgat gtcggcgtcc ttcgccggtt
     3301 gtcaggaaca ggcgatcgct ccgacgttga cgcgtcggcc cattcttcga tctgtcaggc
     3361 acattcatgc aagctcgctc ctcccgtccc cttcttctgg cctttgcctc tgttggcgcg
     3421 atgctcgcct gccccctcgc agcgtcggcc cagacaactc cggcagagcc gcaggatgaa
     3481 agcgcggtgg tcgaggacat cgtcgtcacc ggcacgcgcg ccgtggggcg cagccgcctc
     3541 gacagcatcg ctccggtcga cgtcatctcc gccgagacct tgagccgtca aggcacggga
     3601 accgaactcg ccgccgccct cgccgccact gcgccatcga tcaacttccc ccgtccggcc
     3661 atctccgacg gctcagacca cgtccgtccc gccaccctgc gcggtctcgc gcctgaccag
     3721 acactggtcc tgatcaacgg ccagcgcggc cacgtcggcg cgctcatcaa cgtcaacggc
     3781 gctctcggtc gcggctcgac cgcctttgac ctgaacacca tcccatcggt cagcctcggc
     3841 accgtcgaag tcctgcgcga aggggcctcg gcccagtacg gcgccgacgc catcgccggc
     3901 gtcatcaacc tgcgtctgcg tcaggccgac cacggcggcg gcatcaccgc caactacggc
     3961 atctacaaca ctgatttcac cacggctcgc ggcgatcaca gcgagacgga cggcgaacag
     4021 acgtcggtcg ccggctggat cggcctgccc ctgttcggcg acggcttcct gacactgtcg
     4081 gctgaactgc agaagcgcga gcccaccaac cgctccgact acgccctgcc ctcagcagta
     4141 aacggaggca gcgccaacac tgtgatcggc cgcttcgggg acccttacac cgagggctac
     4201 accggctatt tcaacgcagg caagccgctc agcaacggtt gggacgccta tgccttcggc
     4261 ggttaccagc accgctattc ggaagccgcg gccaccgcac gccaatttaa caacgccaac
     4321 aacgtcccgg ccatctatcc gaacggcttc ctgccgatca tcgcggccag catcgacgac
     4381 tacaacatct acagcggcgt caagggcgcg gccctgggct gggactggga tctttcggtc
     4441 ggctacggcc gcaacgagct tgagtatcgc gtgctcgact cgctcaacgc ttcgtatggt
     4501 gccgcttcaa agaccacctt cgacgcaggt tcgctcagct atggccagtt gaccgtcggc
     4561 atcgacgccg tgaagcagat cgaggctaat ctctatgagc cgcttaacgt ggccctcggt
     4621 gtggaatacc gccgcgagga cttcagcgtc tcggaaggtg agcccgcttc gtacaacaag
     4681 ggccccgtca ccactggcgg ttcaggttca cagggcttcc ccggtttcgc accgtcgaac
     4741 gctgtcgatg aagatcgcag caacatcgcc gcctatatcg acgtggaggg caagctcagc
     4801 gaacagttca gcctcggcgt cgccggtcgc tacgaggact acacggactt cggttcgcag
     4861 ttcaccggca aggtttcggc gcgctacgac ttcacccccg aattcgccct gcgcggcgcc
     4921 atctcgaccg gcttcaagtc gcctgccctt cagcagcagt atttcagcta cgtcgccacc
     4981 aatctgacca cgactgtggt cggcggtgta ccggtcacca ccctttcaca aaacggcacc
     5041 taccgggtcg acgatccgat cgcggtctcc ctcggctcca agccgctgga gcccgagact
     5101 tcaaccaact attcgctggg cgcggtcttc cgccatgaag gctttgagtt gaccgtcgat
     5161 acctaccgca tcgatctcga agaccggatc atctattccg aaaccctcgg cgtctcccga
     5221 ccaagccaga ccgctgtgtc caccgccgcg atccaggccc tgctcgcgcc ttacggcgtc
     5281 accggcgccc gcttcttcct gaacggtgta gacaccacga cctcgggcgt cgatgtggtc
     5341 gcccgttatc gcaccactca ggatatgggt cgcttcgact tcaccctggc gggcaacttc
     5401 aacagcaccg aagtgacccg gaccccggcc gtccccgcca acctggcgat cgcgccatcc
     5461 ggctccttcc tgttcgaccg ctcctccagg ctgtcgttcg aagaaggtac gccgcaacag
     5521 aagatcgtcg ccagcgtcga ttggaccctc ggcgacatgg gcgcgaccct gcgcgccacc
     5581 agctacgaca gcgttctggt cgccaacaac aatgataccc tcgactacga gacgggcagc
     5641 gcggtcaccg tagatctgga aggtcgctac accttcccga tgggcatctc ggcggcgatc
     5701 ggcgtcaaca atctgaccga cgaatatccc actgcaacgc tagccagcct caacggtgtg
     5761 acgggttcgg tgggttatcc gagctactcg ccctatggct tcaacggccg cttcttctac
     5821 ggccgactga gctacgcgtt ctaggaaagc tctttacgac aaacgaccga cggcgcgggg
     5881 agcaattccc cgcgccgttt tcgttgtcga ggcgccttgg tcagccattg ttttgccact
     5941 gagtcctcgc caaacttggc cgtgaactcg aggggaaaat catgttgaag cgcgtcgcag
     6001 cgattgccat cgccgtaagt ctcatcgcca ccgccgcaag cgccatgacg gtgcaggagt
     6061 tcctgaccac cgccagccac atcccgcaaa atcccaccgc actgctgcgc tccgacaccc
     6121 ggcggctgat gggcgagttc agaggcgcgg tgcggactgt gcgagcggag cagactgcgg
     6181 cgacgaccgc aggccgccgg tccccaacct gtgcgcccga gaaggtcagc ttttcggtga
     6241 acgacattct tacccggttc aacgccatcc cggcccaacg ccgtagtcag atcagcgtca
     6301 cccaggcggt gcgcgagtgg atggtcgaac gttacccctg cccggcttga acgactggcc
     6361 tgaacggtga acccgacccc ggatcgctcc gtccgctgac ggcactggtg attttcaccc
     6421 tgtccccaga ctggctcagg ttgtccacgg cgacgggtgc ggccgggcat gtcgtaggcg
     6481 cgtgcgacag gtaagcgtta atgcaatgaa cgccttcacg ccctttcccg atacggtcac
     6541 cgtcccgtcc ctgccgcata atctcgaggc cgagcaggcg ctgctcggcg cgctgatgtt
     6601 cgacaacgcc atcttcgagc gattgtccga ccggctgcgc ggctcgcact tcttcgagcc
     6661 ctttcaccag cggctctacg acgccatcga ggatcacatc cggcagggga tgctggccga
     6721 gccgaccatt ctgatggagc gcttcaagca ggacccggcc tttgccgagt tcggcggcct
     6781 gcgctatctc gccgacctga tggaccgcgc gcctccggga gccaatgcgc ccgactatgc
     6841 gcgcgttgtc tatgacctcg ccctgcgccg cgatctgatc cgcatcggcg gtgacatgat
     6901 caaggaggcg ccggatcccg agacgacggc catcgaccag atcgaacagg ccgaactgac
     6961 cctctattcg ctggccgagt ccggagcccc gtcgtccggt ttcgtcagct tttccacggc
     7021 cttggccggc gccgttcaga tggcaggcga agcgtaccag cgcgaaggca agcttgccgg
     7081 tctggccacc cacctcgacg acctggactc caagctgggc ggcatgcacc cgtccgacct
     7141 tctcatcctc gccggccgtc catcaatggg caagacggcg ctggcgacca atatcgcctt
     7201 caacgtggcc cggaactatc gttgggagcc gacgcccgac ggacgcaaga cggtctcagg
     7261 cggtgtcgtt gccttcttct cgctggaaat gagcgcagaa cagctcgcta tgcggatcct
     7321 cgccgacgct tccggtgtct cgtccgacaa gctgcgtaag ggcgagatcg acgcctccga
     7381 ctttggcaag gtgcgcgatg cggccatcga aatcggtgaa agcccgctct acatcgacgc
     7441 caccggcggt ctggccatct ccaagctggc ggcacgggcg cggcggctga agcggatgga
     7501 gcagggcctc gatctgatca tcgtcgacta ccttcaactc atcaccgtcg gtgaaggcaa
     7561 cggcaacaag aaccgggtgc aggaagtctc cgagatcacc ggcggtctca aggctctggc
     7621 caaggaactc aacgtcccga tcatcgccct gtcgcagctc tcgcgtcagg tagaaaaccg
     7681 cgaggataaa cgtccacagc tgtccgacct tcgggaatct ggctcgatcg agcaagacgc
     7741 cgatgcggtc atgttcgtgt atcgcgagag ctattacctc ggccggacgg agccgcgcga
     7801 gggcacgccc gaacacctgc agtggcagga agacatggac cggctgcagc atcaggccga
     7861 ggtcgtcatc ggcaaacagc gccacggccc tatcggcatc gtcaaactga gcttcgacgc
     7921 cgacaccacc cgcttcggta acctcgcccg cgacggccgg tatggatcca gcttcgccga
     7981 cattcccgag taatttcggc gccgctgtcg accgagttgc ggcataaggc gatcatgagc
     8041 accccggccc ggctgaccat cgatctcaac gcgctcgcgc ggaactatca cacgctggag
     8101 gctgtcagcg gcctgccggt gcatcccgtg gtcaaggcag acggttacgg gctgggcgca
     8161 gcggcagtcg caggtcggct gatggtggaa ggcgcgcgga ccttcttcgt cgcccggggt
     8221 tcagagggcg tggcccttcg acaggcgctg gggcccgagc ccgtcatcta catactcgac
     8281 ggctgccacg gcgatcaggc ggccatgctg aaggctgccg accttcgccc cgtgatcaac
     8341 catggtcagc agcaggcggc atggacggca gctggcggcg gcgcctgcgg tctgcagatc
     8401 gacaccggca tgaaccgctt gggcttccgg ccggaggacg cgcccgaacc gttcgagggt
     8461 ctgtcgctgg tcatgaccca tctggcctgc gccgacgaac cggctgagcc gatgaaccgc
     8521 catcagcgcg atgccttcac cgcagcgagc ctgaaatatc ctggcgtcac ccggtcgttc
     8581 gccaattcca gcggctgttt tctcggggac gatttccgct tcgacgtcgt acggcccggt
     8641 atttgccttt acggcggggg tccggaaggc aaaccggaca gccgcatcgc ccccgtcgcc
     8701 accttgaccg ccgaggtcct tcaaacgcga gacgtgccgg caggcgagag cgtcgggtat
     8761 tcgcgcggct tcatcgccga tcgcccggtg cgcatcgcta cctgcgccac gggctatgcc
     8821 gacggcctga tccgcgcgac cggtacaagc ggctctggtc aggtctggat cagcggccag
     8881 atgcgcccga tccttggccg ggtttcgatg gacgtgctcg ccgtcgacat tacaggactc
     8941 gacgtcgtcg cgggcgacga cgtcgaactg tttggcgcca atcgtctagt cgaccacgca
     9001 gcggccgcgg ccggaaccat cagctatgag atgctgacct cggtcatgcc gcgtgtatcg
     9061 aggacctaca tcggctgatc atcgctagac cgtcgccgta gccagagcag ccgcgccgat
     9121 gaagctggcg aatacggcgc cgacaatgac agcgataatg gtccagatga caatggcagc
     9181 cacgatcacg acggctgtgt aggcgacggt tttttgctcg ggcgacttca tcacgggcgt
     9241 caggcccaaa tagagcaggt acaagccgta gaggctgaag atcacaccga taaccgccag
     9301 cgccgggatc aggctgaaga cgcctccgat ccatgacgcc gtggccgaat agacggcgac
     9361 cttcatggcc gacacgcggt ccttggtccc gtcgaaggtt ggagcaagcg cctcgatcac
     9421 caggcccaac aggaacacgc ctgccagcga caaggcatag cccagcactg ctgcaaccag
     9481 cgccccgatg acgttgccat ggctgaatac gacggagccg atcagaccgc agacggggcc
     9541 gatcgcggcc aggatcatcg catagcggct gaacagagtc gggacggtcg cgaactcgcg
     9601 ctcgatcacc tcccactcga ccttcgggtt cagcaggatg cctttgacgc gggcgaccag
     9661 cgacgggtcg agggcggggg gattatcggt catgtgtttg ggtccggatt ggggtcaaag
     9721 gcataagctc tgccatacct taaggttccg cttatgcctg cgacaatgac ggtcgcaccc
     9781 tcgttcgatc cccctgcccc cccgttcgcg actcctgtaa gccggtacca tggcgagaga
     9841 tggtgcgatc tatgtgtgtc agtcctgtgg ggctgtgcac ggcaaatggg cggggcagtg
     9901 caatgcctgc aacgcgtgga acgtcatcgt cgaggagagc cgttcggccc ctcccggtgc
     9961 gctaaaacca cagagtgctt ccaaaacccg gggccttcag ttcgaatccc tgatgtcgga
    10021 aacgcctgag ccgccccgca tcgtcaccgg cgtcagcgag ttcgaccgcg tctgcggtgg
    10081 cggcgtcgtc ccaggctcgg ccatcctgct gtcgggcgat cccggcgtag gcaagtcgac
    10141 cctgctgctg gatgtcgccg gtcgtgcggc ccttgcgggc gctcgcgtcg cctatatctc
    10201 gggcgaggaa gcgatcgagc agatccgcgc ccgggcaaag cggatggggc tggaaaaggc
    10261 gcccgtcgcc ctcgcctctg ccacggcgct ccgcgacatc ctaggcacgc tgaagcgcga
    10321 gaagttcgac atcgtcatca tcgactccgt ccagaccctg tggtcggacg ctcatgaggc
    10381 cggacccggc tccgtgacgc aggtccgggc ttgcgccggg gaactggtga ggatggcaaa
    10441 agcgggcgga ccggctgtca ttctggtcgg ccacgtcacc aaggacggtc aggtcgccgg
    10501 accccgcgtg gtcgagcata tggtagacgc cgtgctgagc ttcgaaggcg agcgtggcta
    10561 tccattccgc atcctgcgcg ccgccaagaa ccgctttggc gccaccgacg aaatcggcgt
    10621 gttcgagatg ggcgacgccg gactgcgcga ggtcgccaac ccctcggcgt tgttcctagg
    10681 tgaaggcaag gaccgggcgc cgggcgccgc cgtattcgcc ggtatcgagg gcacgcggcc
    10741 cgtgctggtc gagattcagg ccctcgtcgc cccgtcagcc tacggcacgc cgcgccgggc
    10801 ggtgatcggc tgggagacag ggcgtctggc catggtgctg gcggtgctcg aggcgcgttg
    10861 cggcatcggc tttggcaatc aggatgttta cctcaacgtc gcgggcggcc tgcgcatcaa
    10921 tgagccggcg gctgatctgg cggcggccgc ggcgttgatc agttcggcga ctggtgtccc
    10981 cctgcctcaa ggctgtgttg tatttgggga aatcagtctg tcgggcgagg ttcgcgccgt
    11041 cggacgggca gaggcacggc tgcgcgaggc ccagaaactc ggcttcgacc acgtcctgtc
    11101 acaccccctt acggtgaagg gtgacggcgt cctcgtcaca ggcgtcagca gattgacgga
    11161 agcagtcgaa cgaatctcgg aaaaccgcta ttagaactcc acagctgttc gaggattgcc
    11221 tgcttaagtg accggctttg acgccttcgc cattctggtc atcctcgcct cggccgccgc
    11281 cggctgggtt cgaggcggca cgcgtgagct catcaccctg ctcagcttcg tgctggccgc
    11341 cttcctgtca ctggtgtccc tgcctttgac gtcgcgtctt ggtcgcgcgc tgatcgatcc
    11401 cgactgggcg gggaccatct tcgccgcagt ggccgctttc ctgatcatct atttcggtat
    11461 ccgcatcttc ggctcgatgc tgtcaaagcg cgcccaggcc catccgaccc tgggcggtgt
    11521 cgaccgcttc gttggcgttt tcgttggcgc ggcccggtca ctggtcctgc tgggagccat
    11581 ccatctggtc atcgttgccg ccatgccagg cgatcgaaca cctgcctggt tgaaggatgc
    11641 cgccttgcgt cccgtcagcg ccggcgccgc tcgcatgata cagatcatcc tgcccggcat
    11701 cggccgtggc gcagacatca tctcacccgt cgtcgattcc tcagttcgca gagggttttc
    11761 cgacgatggc gccttgcccc ggccccaaac aggcaatact tcgcctcccg tccccgcacc
    11821 ctgaaattcc cgctatcatc ggagccccta cgatgagcag cccaaaatcc gacatccagc
    11881 atgtcatctc agctccggtc gttcaccggg agcatcaccg cgacgtcgat gacgatcgtc
    11941 cgcgtctgga atgcggtgt
//
LOCUS       DASVTM010000016         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_906030,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000016 DASVTM010000000
VERSION     DASVTM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906030"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="mutL"
                     /locus_tag="VF633_00430"
     CDS             <1..1323
                     /gene="mutL"
                     /locus_tag="VF633_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX8469553.1"
                     /translation="QAMAHESVAFTLDLDGRTVLRLPAEHPGDEGRLRRLAALLGRDF
                     EANALLIDQERDGIRLSGYAGLPTYSRGNAAHQYLFVNGRPVRDRLLQGALRGAYADF
                     LARDRHPAAVLFIDIDPLFVDVNVHPAKAEVRFRDPAMVRGLIVGGLRHALAAAGHRA
                     STTVAADALAGFTAHTGAPSPGYPAWNPSSPTATGFSGWQGREAPPARPTTGVLPGLN
                     EQSARVENASPGFASWPSQSGSGAAPDYVDHPLGAARAQVHGTYIVAQTRDGIVVVDQ
                     HAAHERLVYEKMKVQMAAGSVTRQALLTPEVVELDPSEADRVVARADELAELGLVVEA
                     FGAGAVLVRETPALLGDTDVQGLIRDIADDLSEHGAALALTERLGEVCGTMACHGSVR
                     AGRVLSAPEMNALLRQMEVTPHSGQCNHGRPTYVELKLGDLEKLFGRR"
     gene            1320..1781
                     /locus_tag="VF633_00435"
     CDS             1320..1781
                     /locus_tag="VF633_00435"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX8469554.1"
                     /translation="MKGPAILFVCLGNICRSPLAEGAMKAEAARLGLDVEIESAGTGD
                     WHVGHAPDERAQATALNHGIDISGYRGRQVKPADFRHFTHIVALDANNLEDLKRLAPQ
                     NGTAELSLLLDHAGRPGKSVADPYWGDDAGFEATWADVSAGAAGLARALAV"
     gene            complement(1778..>2758)
                     /locus_tag="VF633_00440"
     CDS             complement(1778..>2758)
                     /locus_tag="VF633_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX8469555.1"
                     /translation="AMVVIGGITRLTGSGLSITEWKPIMGALPPMTDVAWADAFQKYK
                     AIPQYTQINAGMSLSAFQGIFWWEWLHRLVGRLVGVVFFVPFIGFLFFKRIPRRLIWR
                     CVILLAMGGLQGLIGWWMVSSGLSVRVDVAPERLTIHLGLALLIFMGLIWTGLEAWAG
                     EEHSRSPVGWSRGATVLLVAVFIQCLLGGLVAGAKAGLIYTDWPMMNGGFFPPVEWGR
                     GAIAFLHDQALVQFNHRIWAYALLIGGTVYAVQSWRWRMAEGLGFWAFLIAASLWLQA
                     ALGILTLINAVPLWLGGLHQAGAAIVLLIATANLWLVRRSQPRLSASRRL"
BASE COUNT          487 a          926 c          928 g          417 t
ORIGIN      
        1 caggccatgg ctcacgagag cgtcgccttc accctcgatc tcgacgggcg cacggtgctg
       61 cgtttgcccg ccgaacatcc gggcgacgaa ggccggttga ggcggttggc cgcattgctg
      121 ggtcgcgatt tcgaagccaa cgccctgctc atagatcagg agcgcgacgg tatccggctc
      181 agcggttacg cgggcctgcc cacctattcg cgggggaacg cggcgcatca gtatctgttc
      241 gtcaacgggc gtcccgtgcg cgaccggttg ctgcagggtg cattgcgcgg cgcctatgcc
      301 gacttcctcg cccgggatcg tcatccggcg gcggtgctgt tcatcgacat cgatccgctg
      361 ttcgtcgacg tcaacgtcca cccggccaaa gccgaggtgc ggttccgcga tccggccatg
      421 gtgcgcgggc tgatcgtagg cgggctgaga cacgcgctgg ccgccgccgg gcaccgggca
      481 tcgacgaccg tggccgccga tgcgctggca gggttcaccg cccatacggg cgcgccgagt
      541 cccggatatc cggcgtggaa cccgtcgtcg ccgaccgcta ccgggttcag cggttggcag
      601 ggccgggaag cgccccctgc ccgtcccacg accggcgtcc tgccggggct caacgagcag
      661 tcggcgcggg tcgagaacgc ctcgccgggg ttcgcctcat ggccgagcca gagcggatca
      721 ggcgctgcgc ctgactatgt cgaccacccc ctcggagccg cccgggcgca ggtccacggc
      781 acctatatcg tcgcccagac gcgcgatggc atcgtcgtgg tcgaccagca cgccgcccac
      841 gaacgtctcg tctatgaaaa gatgaaggta cagatggcgg ccggatcggt gacccggcag
      901 gccctgctga cgccagaggt ggtcgagctg gatccgtcgg aggccgaccg cgtcgtggcc
      961 cgggccgacg aactggctga actcggcttg gtcgtcgagg cgttcggcgc gggggccgtg
     1021 ctggtgcgcg agacccccgc gttgctgggc gacaccgatg ttcagggcct gatccgcgac
     1081 atcgccgatg acctgtccga acacggcgcc gcgttggcgt tgaccgagcg gctgggcgag
     1141 gtttgcggca ccatggcctg ccacggctcc gtgcgcgccg ggcgggtgct gtccgccccc
     1201 gagatgaacg cccttctgcg ccagatggag gtcacgcccc actcgggcca gtgcaaccac
     1261 ggccgaccga cctacgtcga actgaaactc ggcgacctcg aaaagctgtt cggtcggcga
     1321 tgaaaggacc cgcgatcctc ttcgtctgtc tcggcaacat ctgtcgttca ccactggcgg
     1381 agggggcgat gaaggccgaa gcggcgcggc ttggtctgga tgttgaaatc gagtcggcag
     1441 ggaccggtga ctggcatgtc ggccatgcgc cggacgaaag ggcgcaggcg accgcgctaa
     1501 accatggcat cgatatttca ggttatcgcg ggcggcaggt gaagcctgcg gatttccggc
     1561 acttcaccca tatcgtcgcg ctggacgcga acaatctgga agacctgaag cgactggctc
     1621 cgcagaatgg cacggcggaa ctgagcctgt tgctcgacca tgcaggacgc cccggcaaat
     1681 cggtggccga tccgtattgg ggcgatgacg ccgggtttga agccacctgg gccgacgtga
     1741 gcgcaggcgc ggccggactg gccagagccc tcgccgtcta gagccgccgc gacgctgaca
     1801 ggcggggttg cgaccgacgt acgagccaaa ggtttgccgt ggcgatcagc agaacgatcg
     1861 cggcgccggc ctgatgcagg cctccgagcc acagcggcac cgcgttgatc agcgtcagga
     1921 ttccaagcgc cgcctgcagc catagcgagg ctgcgatgag gaaggcccag aagcccagtc
     1981 cttcggccat ccgccaacgc cacgactgga cggcatagac ggtgcctccg atcagcaggg
     2041 cgtaggccca gatgcggtga ttgaactgca ccagcgcctg atcgtgcagg aaggcgatgg
     2101 ctcccctgcc ccattcaacg ggcgggaaga agccgccgtt catcatcggc cagtcggtat
     2161 agatcaagcc ggccttggcg cctgccacca gcccgccgag caggcactgg atgaagacgg
     2221 cgaccagcag gaccgtcgca ccccggctcc agcccaccgg cgagcgcgaa tgttcctctc
     2281 cggcccaggc ttcgaggccg gtccagatca ggcccatgaa aatcagcagc gcgagaccca
     2341 ggtggatggt cagccgctcc ggcgcgacgt cgacccgcac cgacagtccg ctcgacacca
     2401 tccaccagcc gatcaggccc tgaagtccgc ccatggccag caggatgacg cagcgccaga
     2461 tcagccgacg gggaatgcgt ttgaagaaca gaaagccgat gaacggcacg aagaacacga
     2521 ccccgaccag tcgcccgacc agccggtgca accactccca ccagaagatg ccctgaaacg
     2581 cgctcaggct cataccggca ttgatctggg tgtactgcgg gatcgccttg tatttctgga
     2641 aggcgtcggc ccaggcgacg tcggtcatcg gcggcagggc gcccatgatc ggcttccact
     2701 cggtgatcga gaggcctgac ccggtcaggc gcgtgatccc gccaatgacc accatggc
//
LOCUS       DASVTM010000017         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_267696,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000017 DASVTM010000000
VERSION     DASVTM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267696"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1701
                     /gene="clpX"
                     /locus_tag="VF633_00445"
     CDS             436..1701
                     /gene="clpX"
                     /locus_tag="VF633_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX8469556.1"
                     /translation="MTKAAGTDAKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREEHKIGFVKSKDGVPTPKEIREVLDDYVIGQSHAKKVLSVAVHNHYKRLNHAT
                     KNNDVELAKSNIMLIGPTGSGKTLLAQTLARIIDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQASDYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPQQEFLQVDTANILFIVGGAFAGLEKVISARGAGASIGFGAKVSNVD
                     ERRTGDILKGVEPDDLMRFGLIPEFIGRLPVLATLEDLDEEALVTILTEPKNALIKQY
                     KRLFEMENVGLTFTDDAMIAVAKKAITRKTGARGLRSILEGILLETMFELPTFEGVEE
                     VVVNAEVIDGKAQPLLIYAEKKKTADGAA"
     gene            complement(1780..2202)
                     /locus_tag="VF633_00450"
     CDS             complement(1780..2202)
                     /locus_tag="VF633_00450"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEX8469557.1"
                     /translation="MTMIYTAMLAATLLGLAACQTPAGPSTAQETGAVTLSGIANGSA
                     TLQSGQSLSVALPSNGSTGYSWQLAAYDGAVLRPDVPFGEQVTAPHAAGMVGVGGETH
                     WSFEAAAPGTATLLFTYSQPWDKTAPPAETARYTITVR"
     gene            2347..2781
                     /locus_tag="VF633_00455"
     CDS             2347..2781
                     /locus_tag="VF633_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469558.1"
                     /translation="MTDAPIGPAAGPEDDAGPDLGGYDVDDWSRLKAFTGKVARLEDV
                     QAKLAVFALDDTERPWPLDMDLPQPVIWWEEDGEQAAVAIQAENHTGPDGEEMEVLGL
                     ILPDGSAAVALLEDVDLVDDNDPTWRALVSGAVDGVDPDADD"
BASE COUNT          570 a          937 c          894 g          481 t
ORIGIN      
        1 actcgcggac cgaggccgcc ttgatccgtc cccggtcccg gtcgcgggcg tagacggcga
       61 cggccggcgc catcttcacg agccccagcg cccaaccccc agggccagct cgccatcgta
      121 aaaactcggt gaagctgtcg cggatcgtca gtgtaccgtc gaaatcgaac gccacgaccg
      181 attgtccggg cgcgggcctc tggcgcccgg gctctccgct tcccatgtca gacatcagcc
      241 gcaatccccc cccactgtga ccactccccg ctcatcacga actgctcaac cgagcggggt
      301 tgtggcgggc ctttggatgg ttgattgtca ttttttgaat atcttcgcgt ccatagtgac
      361 ggaatcaacg cgacggaagc gcgacggtct caacctcacc tgaggggacg ggctccgcga
      421 gagtgagaag ggtcaatgac caaagcagcc ggcacagacg ccaagagcac gctctattgc
      481 tccttctgcg gcaagagcca gcacgaagtg cgcaagctca tcgccggccc gaccgtgttc
      541 atctgtgatg aatgcgtcga gctgtgcatg gacatcatcc gtgaagagca caaaattggc
      601 ttcgtcaaat ccaaggacgg cgttccgacg ccgaaagaaa ttcgcgaagt cctcgacgac
      661 tacgtgatcg gccagagcca cgccaagaag gtcctctcgg tcgcggtcca caaccactac
      721 aaacgcctga accacgcgac gaagaacaac gacgtcgagc tggccaagtc caacatcatg
      781 ctgatcggcc ccacgggctc cggcaagacc ctgttggccc agacgctggc ccgcatcatc
      841 gacgtgccct tcaccatggc cgacgccacc accctgactg aagccggtta cgtgggtgag
      901 gacgtcgaga acatcatcct gaagctgctc caggcttcgg actacaatgt ggaacgcgcc
      961 cagcgcggca tcgtctacat cgatgaaatc gacaagatca gccgcaagtc ggacaacccc
     1021 tcgatcaccc gcgacgtgtc gggcgagggc gtgcaacagg ccttgctgaa gatcatggaa
     1081 ggcaccgtcg cttctgtgcc gccgcaaggg ggccgcaagc atccgcagca ggagttcctg
     1141 caggtcgaca ccgccaacat cctcttcatc gtcggcggcg cgttcgccgg cctcgagaag
     1201 gtgatctcgg cacggggcgc gggcgcctcg atcggtttcg gcgccaaggt gtcgaacgtc
     1261 gacgaacgcc gcaccggcga catcctcaag ggtgtcgagc ctgacgacct gatgcgtttc
     1321 ggtctgatcc ccgagttcat cggccgtctg cccgttctgg cgacgctgga agatctggac
     1381 gaggaagctc tggtcaccat cctgaccgag cccaagaacg ccctgatcaa acagtacaag
     1441 cgcctgttcg agatggaaaa tgtcggcctg accttcaccg acgacgccat gatcgccgtc
     1501 gccaagaagg ccatcacccg caagaccggc gcgcgcggcc tgcgctcgat cctggagggc
     1561 atcctgctcg aaaccatgtt cgagctgccc acctttgagg gtgtcgagga agtcgtcgtc
     1621 aacgccgaag tcatcgacgg caaggcccag cccctgctga tctatgccga gaagaagaaa
     1681 acggcggacg gcgcggcgta agccccgctt tcagcatgaa acaagaaagg gcgcatcctt
     1741 gcgggtgcgc cctttttgtt gtctcggcgg gctgaaagcc taccgcacag tgatcgtgta
     1801 acgcgcggtc tctgctggtg gcgcggtctt gtcccaaggc tggctatagg tgaagagcag
     1861 cgtcgccgtt cccggcgctg cagcttcgaa actccagtgg gtctcgccgc cgacgccgac
     1921 catgcctgcg gcatgcggag cggtgacctg ttcgccgaaa ggaacgtccg gtctcagcac
     1981 agcgccgtcg tacgcggcca actgccagga atagcccgtc gagccgttac tcggcagggc
     2041 taccgaaagg ctttgacccg actgcagagt agccgagcca ttcgcgatgc cgctcagggt
     2101 aacggcgccg gtctcctgtg cagtcgatgg gccggccggc gtctggcacg ctgccaaacc
     2161 aagcagcgtc gcggccagca tggcagtgta gatcatcgtc atcgcgtctc tcccggcgtt
     2221 ccgcgtcgtc aattgatgct agcgcgactt cggatatctg gcgcgcccga aattcactgt
     2281 tcatgcgtga aggctggcgc cgggcccacc tccggcgtta ggagcgaagc agcaaaggaa
     2341 cgcctcatga ccgacgcccc tatcggcccc gcagccggcc ccgaagatga tgctggcccc
     2401 gatctcggcg gttacgacgt cgatgactgg tcccggctga aggcgttcac cggcaaggtc
     2461 gcgaggctgg aggatgtgca ggccaagctg gccgtattcg cgctcgacga caccgaacgg
     2521 ccgtggccgc tggacatgga cctgccccag ccggtgatct ggtgggaaga ggacggcgaa
     2581 caggcggcgg tggccattca ggccgagaac cacaccggcc ctgatggcga agaaatggag
     2641 gtgcttggcc tcatcctgcc cgacggcagc gcggcggtgg ccctgctgga ggacgtcgat
     2701 ctggtcgacg acaacgaccc gacctggcgc gcgctggtgt ccggtgcagt tgatggcgtg
     2761 gatcccgacg ccgacgactg aggcctacag atcgtagcgc aacagcggcg tgtcgccgcg
     2821 gagatagagc gggtgtttgg ggctgccatc gccgttggtc ccaaaacacc acagcgtcac
     2881 gc
//
LOCUS       DASVTM010000018         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_906167,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000018 DASVTM010000000
VERSION     DASVTM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906167"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2228
                     /locus_tag="VF633_00460"
     CDS             <1..2228
                     /locus_tag="VF633_00460"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEX8469559.1"
                     /translation="TRATVTASGAQLEISRSATGFGWNVTGPVATTMSRLQAGGLDLN
                     GVQGRSSSVRAGGLNGRTEVSAPLTLASDRLQFGELDLKAVRSTADLQVVNDGALQVT
                     ATAAVASPHGAWPLFGPITAEDIPELAEMKLALGDFALSAPSLHLSVGAAGTEVRLTQ
                     PARVKPANGGVMTLTAVQTPVFVAASGQLGGGALSVTSTRGKGLPEATFSVPRWSLTP
                     TGFTADLAGDAALDFGLARTLTVTTKGRLTSSTGRLTYAPSGCSSFTAGLLELGENDV
                     SDVAGQLCPGTVPLVTVADDGWHIRGRFASVSASAPFLEAKVADAAGTVAVDGSKGGD
                     LTLSAAVEQASASDAAAIRRFNPLGLSGTASLAKEQWTGTFDLASSDHPLGQIVLHND
                     GVRESGGLTIDTGMLTFAQGGLQPLDLSPLVAGFISPPVTGQARFTGKFDWSPDTEFS
                     SGVITIPGLDFVSPIGDAKGLKGEIVLTSLVPLTTAANQTVTLDSLAAITPLTDLKLN
                     FGLEASAVTISGADLRVGGGIVRIEPLAIPFDRSQSITGALVFENVQIGNIFSGAGLD
                     DKVKLDAIVSGRVPFTSGPEGVRVVGGVLNAVQPGRLSLQREALTGLEASNNDDSLPP
                     NAMTDLAYQALENLAFDDMTIVVNSLDAGLLNLRFGIHGRHDPPNKQEIRLTWLQVLR
                     RQFFDTPLPLPSGTGVNLNLDTTLNANQLIGDLLELNRVRQGQSAPAPIDAAPISGSR
                     P"
     gene            2306..2494
                     /locus_tag="VF633_00465"
     CDS             2306..2494
                     /locus_tag="VF633_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEX8469560.1"
                     /translation="MISKRTLTAVLLGVVAVGASACVPTVRLEVAPITINATLSADVR
                     IRLDKELQTLLQENPNLF"
     gene            2512..>2767
                     /locus_tag="VF633_00470"
     CDS             2512..>2767
                     /locus_tag="VF633_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HEX8469561.1"
                     /translation="MTFRKYLVLGAAVAMIGVAATGALAQTSAQKSMIDAAKAQGVVG
                     EQADGYLGVVGSVSGSVRAQVDSINIQRRAYYTDLAGRRGA"
BASE COUNT          472 a          954 c          848 g          493 t
ORIGIN      
        1 cgacccgcgc caccgtgacg gcctcgggcg cgcagctcga aatctccaga tcggcaacgg
       61 gtttcggttg gaacgttact ggtcccgtgg ctacgaccat gagccgtttg caagcgggcg
      121 gcctcgacct gaacggtgtg cagggtcggt cgtcatccgt ccgggcgggc ggcctgaatg
      181 gtcgaactga agtgtccgcg ccgttgacgc tcgcgtcgga ccgcctgcaa ttcggcgagc
      241 ttgacctgaa agccgtccgc tcgaccgccg accttcaagt cgtcaacgac ggcgcgttgc
      301 aggtgaccgc taccgccgct gtcgcatcac cccacggcgc gtggcccctg ttcggcccca
      361 tcactgccga ggacattcct gaactggccg agatgaagct tgcgctgggc gacttcgctt
      421 tgtcggcgcc ttcgctgcac ctcagcgtcg gcgccgccgg gaccgaggtc aggttgaccc
      481 aacccgcacg cgtcaagccg gccaacggcg gcgtcatgac cctgacggcg gtccagacac
      541 ccgtcttcgt cgctgcgtcg ggccaactcg gcggcggcgc tctttcagtt acgtccacgc
      601 gcggcaaggg tctgcccgag gcgaccttct cggtccctcg ctggagcctg acaccgaccg
      661 ggttcaccgc cgatctcgcg ggcgacgccg cgctcgattt cggccttgct cgcacactga
      721 ccgtaacgac caagggccgc ctgacatcat cgactggtcg cctgacctac gcgccctcag
      781 gctgcagcag cttcaccgca ggtctactcg aactcggaga gaacgacgtg tccgacgtcg
      841 ccggtcagct atgccccggg accgtccctc tggtgacggt cgcagatgac ggctggcaca
      901 ttcgcggtcg cttcgcctcg gtgtccgcct cagccccctt ccttgaggcc aaggttgcgg
      961 acgccgccgg aaccgtggcg gtcgatggct cgaagggcgg cgacctgaca ctctcggcag
     1021 cagtcgaaca ggcgtcggcc agcgatgctg cggcgatccg gcgcttcaat ccactcggtc
     1081 ttagcggcac ggcgtcgctg gccaaagagc agtggaccgg cacgttcgac ctagcgtcca
     1141 gcgatcaccc gctcggccag atcgtgctgc acaatgacgg tgtgcgtgaa agcggcggtc
     1201 tgactatcga cacaggaatg ctgaccttcg ctcaaggcgg gctgcagccc cttgatctca
     1261 gcccgctcgt cgcggggttc atttcgcctc cagtgaccgg tcaggcccgc ttcaccggca
     1321 agttcgactg gagcccggac accgagttca gttcaggcgt gatcaccatc ccgggcctgg
     1381 atttcgtcag ccccatcggc gacgccaagg gcctgaaggg cgagatcgtc ctgaccagcc
     1441 tcgtcccttt gaccacagcc gcgaaccaga cggtgacact ggacagtctg gcggcgatca
     1501 cgcctctgac cgacctgaag ctgaacttcg gactggaggc ctctgcggtg accatcagcg
     1561 gggcggattt gcgcgtcggt ggtgggatcg tgcgaatcga acccctcgcc atcccgttcg
     1621 accgcagcca gtcgatcacc ggggcactgg tcttcgaaaa cgttcagatc ggcaatatct
     1681 tcagcggtgc gggtctcgac gacaaggtca agctcgacgc catcgtctcg ggtcgcgttc
     1741 ccttcacctc cgggccagaa ggcgtgcgcg tcgtcggcgg cgtgctgaac gcggtacaac
     1801 ccggacgtct gtcactccag cgcgaggccc tcaccggact tgaggcgtct aacaatgacg
     1861 atagtcttcc gcccaacgcc atgacggatc tggcctatca ggcgctggag aacctcgcgt
     1921 tcgacgacat gacaatagtg gtcaacagcc tcgacgccgg cctgctcaac ctgcgcttcg
     1981 gaattcatgg tcgccatgat ccgccgaaca agcaggaaat ccggctgaca tggctgcagg
     2041 tgctgcgtcg ccagttcttc gacaccccgc tgcccctccc ctccggtacc ggcgtgaacc
     2101 tgaacctcga caccacgctg aacgccaatc agttgatcgg cgatctgctg gagctgaacc
     2161 gcgtccgtca gggccagagc gcccccgctc cgatagacgc tgctccaata tccggctcca
     2221 gaccgtgacc gctcggaatg ttcaggggct gttcaccccc gggggcgcta tggtgcggta
     2281 tagtatcgaa ctcggagtag ccgacatgat cagcaagcgg acccttaccg ccgtattact
     2341 cggcgtcgtc gccgtcggcg cttcggcctg cgtcccgacc gtgcgtctgg aagtggcgcc
     2401 gataacgatc aacgccacat tgagcgcgga tgtccgcatc cgtctcgaca aggagttgca
     2461 gacgctcctt caagaaaacc ccaacctgtt ctgatgggaa agattcacca gatgaccttc
     2521 cgtaaatacc tcgttctcgg cgcggcggtc gccatgatcg gcgtcgccgc cactggcgcc
     2581 ctggcccaga cctcggccca gaagtccatg atcgacgccg ccaaggccca gggcgtcgtg
     2641 ggcgagcagg ccgacgggta tctcggcgtg gtcggctcgg tgtcgggcag cgtccgcgcc
     2701 caagtcgatt cgatcaacat tcagcgccgg gcctattaca ccgatcttgc gggccgccgc
     2761 ggcgcca
//
LOCUS       DASVTM010000019         5057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_374030,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000019 DASVTM010000000
VERSION     DASVTM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374030"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1419
                     /locus_tag="VF633_00475"
     CDS             37..1419
                     /locus_tag="VF633_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX8469562.1"
                     /translation="MLGSDLHVTSLASLPPDSLASALASLTEAEAEQLLHDWRFTARP
                     EQTAPEGDWQVWAYIAGRGAGKTRSGAEWVREKIKGGARRLALIAPTAGDARDVMVEG
                     DSGVLSVCWDGDRDHKGRITGRPLYEPSKRRLTWANGAMATTYSADEPDRLRGPQHDA
                     GWCDELAAWTYAQEAWDMFMFGLRLGDHPQAMVTTTPRPIPLVRQIVTAASTVVTKGS
                     TYSNRAHLAPSFFDKIITRYEGTRLGRQELAGEILDDVPGALWTRGMVDSARSVGTLP
                     DMKRIVVAVDPSGTSGAGDDGDAIGIVVAGKGVDGLAYVLADRSCKLSPDGWGQRTVN
                     AYREFSADRIVGERNFGGAMVEHVIRTVDRSASYAEVTASRGKVVRAEPIAALYEQGR
                     VKHVAGLEALEDQMCAMTGDGFLGEGSPDRVDALVWALTELDLQSVKRAEKPKTEARR
                     EPRGPTAWMG"
     gene            1461..3410
                     /locus_tag="VF633_00480"
     CDS             1461..3410
                     /locus_tag="VF633_00480"
                     /inference="COORDINATES: protein motif:HMM:NF027825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEX8469563.1"
                     /translation="MPKRDGNPGPTDPDPKVIDRTEFLTEARERFQQGVDYDQDNRNA
                     ALEDLRFLTGEQWDPAVVKVRAGRPCLTINRLPQFVAQVVGDIRLNQPAIRVRPAEDG
                     DVEVAETRQGLIRGIEYASKAQRVYASAGQSQVSCGLGHFRVKLEYASDDVFDQDIRI
                     RSIPDPFAVVWDPMATEPTGADAEWCFVVDEIDRKTFEAAWPDAQSSDLTVGEAQGWS
                     TKDTVRVTEYWRMQETTRTLAAMPDGFVVDVTDQPELAEQAVRTRQVTRRSAVMELIT
                     GAAVLSTQAYELPISRLPIFRVPGWEMHIRDKRVRWGLVRFAKDPQRLMNYDRSVFAE
                     TMGLAPKAQWIASSEAIEGIEDDFRSAHRSGDPLLVYNTGAEKPQRVDPPAFPAGLAQ
                     SAAMHAQDMKDVTGLHDASLGAQSNETSGKAIMARDRQGDVATYIYPDNLKDAIGECG
                     RVVNELIPVVYDTARTIRVLGDDGKAEQKRINDPNTEGSIDLLIGKYDVVVESGPSYS
                     TKRVEAAESMMAFVQAVPTAGAVAGDLIAKAQDWPMADEIAKRLVKTLPPGIREPEEG
                     EEPEQPPPPDPMQQQQAQMAMQAGQLDLRLKAAQAEKAEADARRAEAEAFKAEMEVQL
                     AQAGSMIGQMAPPPFEQPAPAAPGF"
     gene            3430..4242
                     /locus_tag="VF633_00485"
     CDS             3430..4242
                     /locus_tag="VF633_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469564.1"
                     /translation="MTDVTNPEAQAPEEPQTLPEGQQEGQEQQPADDPGASETETPEQ
                     RQERKRQSFQERIDEKTRLQREAERDRDYWREQATRNQPPPQAQPEPTNGEPDPSQYE
                     YGETDARFIRDLARYETRQEFTAQRQQDEAQRSQAQLLSTFSTNAAKAAETMPDFHEV
                     VGDDYGRAAALCTPAMSAAILESADGPAVAYHLTKNPTEARRIAALSPLAQVREIGRL
                     EGRLSAPTVAQPAPRPSNAPPPPASARGASGQFKVSPETDDFAAFAKQYASG"
     gene            4270..>5057
                     /locus_tag="VF633_00490"
     CDS             4270..>5057
                     /locus_tag="VF633_00490"
                     /inference="COORDINATES: protein motif:HMM:NF023081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HEX8469565.1"
                     /translation="MANALLSPKVYANTALALLTNQLVAAKMIDGQFSDTFQKKGDTI
                     YVKRPPEFTLRTGPVVSIQDVVEGEQAVKIDRQIGVDFQFTSVEETLTVDSLMKSRSM
                     KAATAQIAQGVDSVILGQYVNFPNWVGTPGQIIDSTQDFSLGPKRLDNLGVPMDGRFA
                     LMGPNDHYGLVGNFVGLNTQEGVANQALKEARLPRVQGVDPYMTQGMPTHTTGTRTNG
                     TIAGAAQQTTYVAAKATYTQTLNLAGLGAGGTVKAGDVFSIAGV"
BASE COUNT          953 a         1661 c         1739 g          704 t
ORIGIN      
        1 acaagagcga gaacacgacc aaggtcgagg tgacggatgc tagggagcga cttgcacgtc
       61 acgtcgctgg cgtcgttgcc gccggactcg ctggcgagcg ctctggcctc actcactgag
      121 gcagaagccg agcagctact gcacgactgg cgcttcactg cgcggccgga gcagacagcg
      181 ccggaaggcg actggcaggt ctgggcctac atcgcaggac gcggagccgg gaagacgcgt
      241 tcaggagccg agtgggttcg ggagaagatc aagggcggcg ctcgacggct ggccctgatc
      301 gcgccgacag cgggcgatgc tcgcgatgtc atggtggaag gcgacagcgg cgtgctcagc
      361 gtgtgctggg acggcgaccg cgaccacaag ggccggatca ccggaaggcc gctgtacgag
      421 ccctccaagc gccgcctgac gtgggccaac ggggcgatgg cgacgaccta ctcggcggat
      481 gagccggatc gcctaagagg gccgcagcac gacgccggat ggtgcgacga gctcgcggct
      541 tggacctacg ctcaagaggc gtgggatatg ttcatgttcg ggctccggct cggcgaccat
      601 ccgcaggcga tggtgacgac gactccgcga ccgatcccgc tggtgcggca gatcgtgaca
      661 gcggcctcta cggtcgtgac caaggggtcg acctacagca accgggcgca cctggccccg
      721 tcgttcttcg acaagatcat cacccgctac gaaggcacgc gcctcggtcg gcaggagttg
      781 gccggcgaga tactggacga tgtgcctggc gcgctctgga cgcgcggcat ggtcgacagc
      841 gctcggtcgg tcggaacgct gcccgacatg aagcgcatcg tcgtggccgt ggacccctcg
      901 ggaacgtcgg gcgctggcga tgacggtgac gctatcggga ttgtggtcgc gggcaagggt
      961 gtcgacgggt tggcctacgt cctggcggac cggagctgca agctttcgcc ggacggctgg
     1021 ggacagcgca ccgtcaacgc ctatcgcgag ttcagtgcgg accgcatcgt cggtgagcgc
     1081 aacttcggcg gcgcgatggt ggagcatgtg atccgcaccg tggatcgctc cgccagctac
     1141 gcagaggtca cggcgtcacg gggcaaggtc gttcgggctg agcctatagc ggcgctctac
     1201 gagcagggcc gggtcaagca cgtcgcgggg cttgaggcgc tggaagacca gatgtgcgcc
     1261 atgaccggcg acgggttcct cggcgagggc tcgcctgacc gtgtcgatgc gctggtctgg
     1321 gcgctgaccg agctggacct gcagtccgtc aagcgcgctg agaagccgaa gacagaagcc
     1381 cgccgcgagc cgcgaggacc gaccgcatgg atgggctgag gcgagacgac aaccctggac
     1441 ccagtgacgc tggcccgctc gtgccgaagc gcgacggcaa ccccggtccg accgatcccg
     1501 atcccaaggt catcgaccgc accgagttcc tgacggaagc ccgcgagcgt ttccagcagg
     1561 gcgtggacta cgatcaggac aaccgcaacg ccgcgctgga agacctgcgg ttcttgaccg
     1621 gcgaacagtg ggacccggcc gtcgtcaagg tgcgcgccgg gcggccgtgt cttaccatca
     1681 accgcctgcc gcagttcgtg gcgcaggtag tcggcgacat ccgcctgaac cagccagcca
     1741 ttcgggtgcg gcctgcagag gatggtgacg tcgaggttgc ggagacgcgg caggggctga
     1801 tccgcggcat cgagtacgcg tccaaggcgc agcgggtcta cgcctccgct gggcagtcgc
     1861 aggtgtcgtg cgggctcggc catttccggg tgaagctcga gtacgccagc gacgacgtgt
     1921 tcgatcagga cattcgtatc cggtcgatcc ccgatccctt tgcggtcgtg tgggatccga
     1981 tggcgacgga gccgaccgga gccgacgcgg agtggtgctt cgtggtcgat gagattgacc
     2041 gcaagacgtt tgaagccgcc tggccggatg ctcagtcgag tgacctgacg gtcggggagg
     2101 cgcagggctg gtccaccaag gacacggttc gcgtcaccga gtactggcgg atgcaggaga
     2161 caacgcggac gctggcggcc atgccggacg gtttcgtggt cgacgtcacc gatcagcccg
     2221 aactcgcgga gcaggcggta aggacgcggc aggtgacgcg ccgatccgcc gtcatggagt
     2281 tgatcaccgg cgccgcggtg ctgtcgacgc aggcttacga gctgccgatc agccgtctgc
     2341 cgatcttccg ggttccgggc tgggaaatgc acatccgcga caagcgggtg cgctggggct
     2401 tggtgcgctt cgccaaagac ccacagcggc tgatgaacta cgaccgctcg gtgttcgccg
     2461 agacgatggg tctggccccg aaagcccagt ggatcgcgtc gtcggaggcc atcgagggca
     2521 tcgaggacga cttccgcagc gcgcaccgat cgggtgatcc gctgctggtc tacaacacgg
     2581 gtgccgagaa gccgcagcgc gtcgatccgc ccgcgttccc ggctggccta gcgcagtccg
     2641 cagcgatgca cgcgcaggac atgaaggacg tgaccgggct gcacgacgct tcgctgggcg
     2701 cgcaatccaa cgagacgagc ggcaaggcga tcatggcccg cgaccgtcag ggcgatgtcg
     2761 cgacgtacat ctacccggac aacctcaagg acgcgatcgg cgagtgcggc cgggtggtga
     2821 acgagctgat cccggtcgtc tacgacaccg cgcgcacaat ccgggtgctg ggtgacgacg
     2881 gcaaggctga gcagaagcgg atcaacgacc cgaacaccga aggcagcatc gacctgctga
     2941 tcggcaagta cgatgtggtc gtggaaagcg ggccctcgta ctccaccaag cgcgtcgagg
     3001 ccgccgaatc catgatggcg ttcgtccagg cggttccgac tgccggggct gtagcgggcg
     3061 acctgatcgc caaggcgcag gactggccga tggcggacga gatcgccaag cggctggtca
     3121 agacgctccc gccgggcatc cgcgagccgg aagagggcga ggagcccgaa cagccgcctc
     3181 cgcccgaccc gatgcagcaa cagcaggcgc agatggcgat gcaggcgggt caactcgacc
     3241 tccgcctcaa ggccgcgcaa gccgagaagg ccgaagccga cgcccgcaga gccgaagcgg
     3301 aggcgttcaa ggccgagatg gaagttcagc tcgcacaagc gggctcgatg atcgggcaga
     3361 tggcgccgcc gccgtttgaa caacccgcac cggcggctcc gggcttctag ccctcgccac
     3421 aggcgctcca tgaccgacgt gaccaatccc gaggcgcaag ccccggaaga gccccaaacc
     3481 ctgcctgaag gccagcaaga ggggcaagag cagcaacccg ccgacgatcc cggcgcatct
     3541 gagacggaga cgcccgagca gcgccaagag cgcaagcggc agtcgtttca ggagaggatc
     3601 gacgagaaga cccgcctcca gcgtgaagct gagcgggatc gggactactg gcgcgaacaa
     3661 gcgacgcgca accaaccgcc gccccaggcg caaccggaac cgacgaacgg cgagcccgat
     3721 ccgtcgcagt acgagtacgg ggaaaccgac gcccgcttca tccgcgacct cgctcgatac
     3781 gagacgcggc aggagttcac ggcgcaacgc cagcaggacg aggcccagcg ttcccaagcg
     3841 cagctgctgt ccacgttcag cacgaacgcc gccaaggccg ccgagaccat gcccgacttc
     3901 catgaggtcg tcggggatga ctacggccgc gcggcggctc tctgcacccc ggccatgagc
     3961 gccgccattc tggaaagcgc ggacggtccc gccgtcgcgt accacctcac caagaacccg
     4021 accgaagcgc gccgcatcgc ggctctgtca cccctcgctc aggtccgtga aatcggccgt
     4081 ctggaaggtc gcctgagcgc gccgaccgtg gcccagcctg caccccgccc atcaaacgct
     4141 ccccctccgc cagcctctgc ccgcggcgca tccggccagt tcaaggtttc ccccgagacc
     4201 gacgacttcg cggcgttcgc caagcagtac gcgagcggct gagcacccat cctcgaaagg
     4261 acgccttaag tggcaaacgc tctcctctcc ccgaaggtct acgcgaacac cgcgttggcc
     4321 ttgctgacca atcagctcgt cgcggccaag atgatcgacg gccagttcag cgacaccttc
     4381 cagaagaagg gcgacacgat ctacgtgaag cgcccgccgg agttcacgct ccgcaccggc
     4441 ccggtcgtgt ccattcagga cgtggtcgag ggcgagcagg ccgtgaagat cgaccggcag
     4501 atcggcgtcg acttccagtt cacctcggtc gaggagacgc tgacggtcga tagcctgatg
     4561 aagtcccgct cgatgaaggc ggccaccgcc cagattgcgc agggcgtcga ttcggtgatc
     4621 ctgggtcagt acgtgaactt ccccaactgg gtcggcacgc cgggccagat catcgactcc
     4681 acgcaggact tctcgctggg tccgaagcgt ctcgacaacc tcggcgtccc aatggatggc
     4741 cgcttcgccc tgatggggcc gaacgaccac tacgggctgg tcggcaactt cgtcggcctg
     4801 aacacgcagg agggcgtcgc caaccaagcg ctcaaggaag cgcgtctgcc gcgcgtgcag
     4861 ggtgtcgacc cctacatgac gcagggcatg ccgacgcaca cgaccggcac gcgcaccaac
     4921 ggcacgatcg ccggtgccgc gcagcagacc acatatgtcg cagccaaagc gacctacacg
     4981 cagacgctga accttgcggg gctcggcgcc ggcggcacgg tcaaggccgg agacgtgttc
     5041 agcatcgcag gcgtgtt
//
LOCUS       DASVTM010000020         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_746652,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000020 DASVTM010000000
VERSION     DASVTM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746652"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..909)
                     /locus_tag="VF633_00495"
     CDS             complement(52..909)
                     /locus_tag="VF633_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX8469566.1"
                     /translation="MAIGVFDSGVGGLTVHRELVARFPERDFVYFADQANAPIGGKTG
                     EQIVDLTRAGCTTLFDAGASVVVLACNTASAVALRRLQQTWIPEQQAKYGRAVNVLGI
                     IVPTIEAATGLPWTYETDRTAKDDKIEAIDIVGVFSTAATALSRVYEIEIDKRREDLA
                     VFSESCPGLAGLIELGATPEELKVVVDEHVDALRRRIGRHPDTAILGCTHYEIIADLF
                     VQALPKSTTLIDQPRSVADALARYFEHHPDYDLGSSGRRDFLTSGEPGPQSEMVSQFW
                     GAPLSFQAA"
     gene            1031..>1888
                     /locus_tag="VF633_00500"
     CDS             1031..>1888
                     /locus_tag="VF633_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family peptidase"
                     /protein_id="HEX8469567.1"
                     /translation="MHRRQLLIAGGSLMALSACASGGGTADRMSGEYAAGSNPATVAE
                     EARIARAVLPATAPRAELLQVWTGPYDGVPPWDKVTPAKLREALMEGMTLNRADVAAI
                     ANNPEAPTFANTLVAMELMGAPLDRVSNIYSVMTGNIGGAEYDALDTEMSPILSAFFD
                     EISFNEKLFQRVKAVADNADAMGLNAQQKRLAVRSRDNFIRSGANLDAAGKAELGRIN
                     TALSGAFTSFGQKVVADENAWTVIPNEAGVAGLPASNKAAAASAARGKSIPGWIIMNT
                     RSSVDPFLTF"
BASE COUNT          348 a          638 c          591 g          311 t
ORIGIN      
        1 cagctcagtg agttcgagga ctagatcgtg gcttgaagcc tgcgcgtgaa atcaagccgc
       61 ctgaaaactc agcggcgcgc cccagaactg gctgaccatc tccgactgcg gtccgggttc
      121 gccgctggtc agaaagtcgc gccgtccgga tgatcccaga tcatagtcgg gatgatgctc
      181 gaaatagcgc gccagtgcat cggcgacgga acgtggctga tcgatcagcg tcgtgctctt
      241 cggtagcgcc tgaacgaaca gatcggcgat gatctcatag tgggtgcacc cgaggatggc
      301 ggtgtcggga tgccggccga tacgacggcg cagggcatcg acatgctcgt ccacgacgac
      361 cttgagctct tcgggcgtcg cgcccagttc gatcaggccg gccagaccgg ggcaggactc
      421 cgagaacacc gccagatctt cgcgccgctt gtcgatctcg atctcataga cgcggctgag
      481 cgccgtggcg gcggtcgaga acacgccgac gatatcgatc gcctcgatct tgtcgtcctt
      541 ggcggtccgg tcggtctcat aggtccaggg caggccagtc gccgcctcga tggtcgggac
      601 gatgatgccg agcacgttca ccgcgcgacc gtatttcgcc tgttgctctg gaatccacgt
      661 ctgctgcaag cgacgcaggg ccacggccga cgctgtgttg caggccagca cgacgacact
      721 ggcaccggcg tcgaacaatg tggtgcagcc tgcgcgcgtc agatcgacga tctgctcgcc
      781 ggtcttgccg ccgatcggcg cattggcctg atcggcgaaa tacacgaagt cccgctccgg
      841 aaaccgggcc acgagttcac ggtggaccgt caggccacca acaccggagt cgaatacgcc
      901 tatcgccatg gcgcgggctt tagccgcacg ggcgtcgggc gtccaacgcc aaagattact
      961 gtccgttcag gtgacggtca ggttcaacag ggctagtgtc cacgcaaatg attttgatcc
     1021 ggagcattcc atgcacagac gtcagcttct catcgcgggc ggcagcctga tggccctctc
     1081 ggcctgcgcc agcggcggcg ggaccgctga caggatgtct ggcgaatacg ccgccggctc
     1141 gaacccggcc acggtggcgg aagaagcgcg catcgcccgc gccgtcctgc cggccaccgc
     1201 gcccagggcc gaactgctgc aggtctggac cggtccctat gacggcgtgc cgccgtggga
     1261 caaggttacc cccgccaagc ttcgcgaagc tttgatggaa ggcatgacgc tcaaccgcgc
     1321 cgacgtcgcc gccatcgcca acaaccccga ggctccgacc ttcgccaaca ccctcgtggc
     1381 catggagctg atgggcgcac cgctggatcg cgtgtccaac atctacagcg tcatgacggg
     1441 caacatcggc ggcgccgaat acgacgctct cgacaccgag atgtcgccga tcctctcggc
     1501 cttcttcgat gagatcagct tcaacgaaaa actgttccag cgcgtcaagg ccgtggccga
     1561 caacgccgac gccatgggcc tgaacgccca gcagaagcgt ctggccgtgc gcagccgcga
     1621 caacttcatc cgctcgggcg ccaatctcga cgccgccggc aaggctgagc tgggccgcat
     1681 caacaccgcc ctgtcgggcg ccttcaccag cttcggccag aaggtcgtcg ccgacgagaa
     1741 cgcctggacc gtgatcccga acgaggcggg cgtcgcaggc ctgcctgcat cgaacaaggc
     1801 cgccgccgcc tccgccgcgc gcggcaagtc gatcccgggc tggatcatca tgaacacccg
     1861 ctcgagcgtg gatccgttcc tgaccttc
//
LOCUS       DASVTM010000021         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_480315,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000021 DASVTM010000000
VERSION     DASVTM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480315"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF633_00505"
     CDS             complement(<1..313)
                     /locus_tag="VF633_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469568.1"
                     /translation="MATFFALQVGLAGAPDPATGYLCNIKRVDAAVRARGAPVLQALL
                     AEGTFTHARVLGQLYGELRDAWPGVTLERLRLRLSPYMAIEHFAKEPTMIRLSQRFEF
                     SA"
     gene            604..1548
                     /locus_tag="VF633_00510"
     CDS             604..1548
                     /locus_tag="VF633_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX8469569.1"
                     /translation="MAKKVSKKPVVKPVVKAKPKTVSVAASSGAGRKASAKSPRVVSR
                     AAKPAAKAGVSRSSAKSTVVKSPVARTPVARPVAQAPTRPAVALKALSRAGRSASTPI
                     PNPAPNPAASPTPSSSALKTGAPKLVIRSSAKPELVIKSSASPAMKSGLVKSLTRPKV
                     KTRTLEPIIVRLPPPPVTSNSKMAKNQAGLSTKELETFRDLLLQKRRELLGDMSSMEG
                     DALRSGNTNLSTLPLHMADSGTDNYEQEFTLNLVEKERKLLGEIQRALAKIQDGSYGV
                     CEGTGKPIGKPRLEAQPWAKYSIEHARALEKPQYRRAY"
BASE COUNT          317 a          554 c          537 g          245 t
ORIGIN      
        1 cggcggagaa ctcgaatcgc tggctcaggc ggatcattgt cggttccttc gcgaagtgtt
       61 cgatcgccat gtacggagag aggcgcagcc gcagccgctc gagcgtcacg cccggccagg
      121 cgtcgcgcaa ctcgccgtag agctgcccta aaacccgggc gtgggtgaac gtgccctcgg
      181 ccagcagggc ctgcagtacc ggcgcgccgc gggcgcggac ggcggcgtcg acgcgcttga
      241 tgttgcacag gtagcccgtc gccgggtcgg gcgcgccggc gaggcccacc tgcagggcga
      301 agaacgtcgc caatggatcc attgggggcg ttcctgcgta cccattgggt gacaatgccc
      361 ccccggtggg catcgcggcc ggggcggcgg tgacgtggag acgaacctcg cggacgagtc
      421 gggccatggg acgtgattgt aagcgaaccg gcggcgacat tatcggctgc cgtccgttag
      481 gccgacgttc ggccgaaaat cttgaccgaa gtcgagggat ctgataggtt tgcattgctc
      541 tacccggggc ggcggagcgc gtggctaatc gactgaaaag tccaagcacc agaggcacaa
      601 tccgtggcga agaaggtcag caagaagccg gtcgtaaagc ctgtcgttaa ggcaaagccc
      661 aaaaccgttt ccgttgcggc cagttccggc gcgggcagga aggcgtccgc caagtcgccg
      721 cgcgtggtgt cgcgggccgc caagcctgcc gccaaggccg gtgtctcccg gtcttccgcc
      781 aaatcgactg tggtcaaatc gcctgtcgcc aggacaccgg tcgcccggcc cgtcgcacag
      841 gcgccgaccc gcccggcggt ggcgctcaag gccctgtcgc gggccggccg ttcggcgtcg
      901 acgccgatcc caaacccggc cccaaatccg gccgcgagtc cgaccccgag ctcttcagcg
      961 ctcaagaccg gcgcgcccaa gctcgtcatc aggtcgtcag cgaagccgga actggtcatc
     1021 aaatcgtcgg cttccccggc catgaagtcc gggctggtga agtcgctgac gcggccgaag
     1081 gtcaagacac ggacgctcga gccgatcatc gtgcgcctac ccccgccgcc ggtcaccagc
     1141 aacagcaaga tggccaagaa ccaggccggc ctgagcacga aggaactcga aaccttccgc
     1201 gacctgctcc tgcagaaacg ccgtgagctg ctcggcgaca tgagcagcat ggaaggcgat
     1261 gccctgcgct cgggcaacac caacctcagc accctcccgc tacacatggc cgacagcggc
     1321 acggataact acgagcagga attcacgctc aacctcgtcg aaaaagagcg caaactgctc
     1381 ggcgagatcc agcgtgcctt ggccaaaatc caagacggca gctacggcgt ctgcgaaggc
     1441 acgggcaagc ccatcggcaa gccgcgcctc gaagcccagc cgtgggcgaa gtactcgatc
     1501 gagcacgccc gggcgcttga aaagccccag tatcgccgcg cgtattaacc ccgcgcaggc
     1561 ccgacgttcg gagcgcattc acccactggc ggggtcactg tagtgacccc gtctttttct
     1621 tcaggggtag gtttctcgtt tcaacggggt cgc
//
LOCUS       DASVTM010000022         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_55167, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000022 DASVTM010000000
VERSION     DASVTM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55167"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VF633_00515"
     CDS             complement(<1..805)
                     /locus_tag="VF633_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX8469570.1"
                     /translation="MRLDGPTADSRWPRAWVNWRAAVLLGLISSTFSTVVSTLGAQRI
                     GRDAPVDWMVVASIPARDAALDVHPQLWAQVVGVLFHQWADFSWAVVFFGFLGFWTAR
                     LKPGWIAVIALPWSVATSSSEWLFLVPWLPFQQPIFTLEQPYWLGFFVHLMSALIYPL
                     FPWIRDQVEGLRPSPHRRFAAIWGGGATTFVALLAVVAIMGAADREPGWRGGGDPQAD
                     QTFLRRMAAHHVQGVEIATLAATRAEDPHLRALAGLMAAAQSGEIRVMQA"
     gene            complement(807..1835)
                     /locus_tag="VF633_00520"
     CDS             complement(807..1835)
                     /locus_tag="VF633_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8469571.1"
                     /translation="MGCRILGAIAFILLLGPGLTACATSTGLTSGDPDRIAGRTYVVT
                     GASSGFGRGTAVRLGALGANVVLAARRTELLEEVASEVRAAGGGALVVQTDVADAAAV
                     QRLADQAVGRFGRVDVWINNAGVGAIGRFEDIPIEDHSRLVDINLKGVIYGSHAAMRL
                     FRAQGFGELVNVGSVDSEVPLAYQASYSATKAGVLALGRAMNEEIRLNRTGRIHVSTV
                     MPWAADTPWWPHAANYSGGTPRMAAMDDPQKIVDAIVWVSVHPREELPVGWKAKASND
                     MHHLFPDITERMSGDIAHRWQIETAPPAPPTHGTLHEPMLEGRAVDGGVRERMAAENE
                     ARRVQTPK"
     gene            2033..2590
                     /locus_tag="VF633_00525"
     CDS             2033..2590
                     /locus_tag="VF633_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8469572.1"
                     /translation="MKDVVLAASAAAAVLLAACSTMPTGQAAPIAEAAMAQSAPPAME
                     FVRMAGASDQYEIQSSQLVLQSTQDPELRRFAQMMVDHHTMTTATVMRAAQTAGLTPS
                     PPTLDAEKTEMIRQLQGTSNEARDALYKQQQVMAHRQALMLHASYAKNGDAPSLKTAA
                     ASAVPIVSRHYNMIMAMAGDASSPM"
     gene            3303..>4317
                     /locus_tag="VF633_00530"
     CDS             3303..>4317
                     /locus_tag="VF633_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8469573.1"
                     /translation="MAGDKLAKYQEMRDFGATAEPSGKDAKVVPSEALRFVIQKHAAS
                     HLHFDLRLEYEGTFRSWAVPKGPSLDPRDRRMAMEVEDHPLDYGDFEGTIPKGQYGGG
                     TVMLWDRGFWAPEKGFEKIGAALAKGELKFVMEGERMHGSWVIVRTRRDDRGKATWLL
                     IKHRDEGAVEGNISGPTDDDRSVASGRTMTEIANGKGKAAKPFMTAKGAAAGSVWQSN
                     RDDTAPAGLIAPTRSRPPAKSKAVSELPDFVEPQLCKSLEKPPGGAGWAHEIKFDGYR
                     MQLRTAGGKATLSTRKGLDWSARFPEIVAAGTALKDGIIDGEVVALDHTGAPDFAALQ
                     AAIA"
BASE COUNT          817 a         1359 c         1409 g          732 t
ORIGIN      
        1 acgcctgcat gacccggatt tcgccggact gggccgccgc catcagaccg gccaacgccc
       61 gcaaatgggg atcctcggcg cgtgtagcgg ccagtgtggc gatctcgacg ccctgaacat
      121 gatgcgccgc catccggcgc aggaaggtct ggtcggcctg aggatcgccg ccgccgcgcc
      181 atcccggttc gcgatccgca gcgcccatga tcgccaccac ggcgaggagg gcgacgaatg
      241 tcgtcgcccc gccgccccag atcgcggcga acctccggtg cggagagggt cgcagcccct
      301 ccacctgatc ccggatccag gggaacagcg ggtagatcag ggctgacatc aggtggacga
      361 agaaccccag ccaatagggc tgttcgagag tgaaaatcgg ctgctggaag ggtagccacg
      421 gaacgaggaa caaccattcc gacgacgagg tcgcgacgga ccatggcagg gcgatgacgg
      481 cgatccagcc cggcttcagt cgggccgtcc agaaccccag gaagccgaag aacaccaccg
      541 cccaagagaa gtcggcccat tggtggaaga gaacgccgac gacctgcgcc cagagctgcg
      601 gatgcacgtc cagtgcggcg tcacgcgccg gaatgctcgc caccaccatc cagtctacgg
      661 gcgcgtcacg tccgatgcgt tgggcgccga gggtgctgac aacggtcgag aaggtgctgc
      721 tgatcaggcc aagcaggacc gcggctcgcc agttaaccca tgcgcgcggc caacggctgt
      781 cagcggtcgg accatccagc ctcattctac ttcggcgttt gaacgcggcg ggcttcgttc
      841 tcggcagcca tcctctcacg gacgccgccg tcgacggccc ggccttccag catcggctcg
      901 tgcagggttc cgtgagtcgg cggggcgggg ggcgcggtct cgatctgcca gcgatgtgcg
      961 atatcgcctg acatccgttc ggtgatgtcg gggaacaggt ggtgcatgtc gttcgacgcc
     1021 ttggccttcc agccgacggg caactcttca cgggggtgaa ccgaaaccca gacgatggcg
     1081 tctacgatct tttgcggatc gtccatcgcc gccattcgcg gcgtgccgcc gctgtagttg
     1141 gcggcgtgag gccaccacgg ggtgtcggca gcccagggca tgacggtgga gacgtgaatc
     1201 cgccccgtcc ggttcagccg gatctcctcg ttcatcgccc tgcccaaggc gagcacgccc
     1261 gcctttgtgg cggagtagga cgcctgatag gcgaggggga cttcgctatc gacggagccc
     1321 acgttcacca gttcgccaaa accctgggcc cggaacagac gcatggcggc gtgactgccg
     1381 tagatgacgc ctttcaggtt gatgtcgacc agccttgaat ggtcctcgat cggtatatcc
     1441 tcaaagcgac cgatcgcgcc gaccccggcg ttgttgatcc agacgtccac gcgcccgaac
     1501 cgcccgacgg cctgatcagc gagacgctgc accgcggccg cgtccgccac gtcggtctgc
     1561 acgaccaggg ccccaccacc ggccgcccgc acctcggacg ccacctcttc gagcagttcc
     1621 gttcgccggg cggcgagcac gacattcgcg ccaagggcgc ccagccgcac ggcagttcct
     1681 cgcccaaagc cgctggaggc gccggtcaca acataggtgc ggcccgcgat ccgatcagga
     1741 tcgccactgg tcagtccggt cgatgtcgcg caggctgtca ggccggggcc caaaagcaga
     1801 atgaaggcta tcgccccaag aattcgacag cccatcaaat agtatccgcc aacttcgatc
     1861 tctgttgtgc gggaaacaag tattacttgc tacggttcct atgtcggaaa cgacacacaa
     1921 agctacaggc gtgttcaggc gcggtcggaa tgttgtagac ccgactctgg aactgttttt
     1981 atggtccggg tcttgtgtcg aacgatgcgt tttggcgtcc aggagatcct ttatgaaaga
     2041 tgttgtactc gcggcctcgg ccgctgcggc agtgttgctt gctgcatgca gcacgatgcc
     2101 aacaggccaa gcggcgccga ttgccgaggc cgccatggcg caaagcgcgc cccccgcaat
     2161 ggagtttgtc cggatggccg gggcgagcga ccagtatgaa attcagtcta gccagctcgt
     2221 gttacagtcg acccaggatc ccgagttgag acggttcgcg cagatgatgg tcgatcacca
     2281 tacaatgacg accgcaacgg tgatgcgcgc ggcccagacc gcaggcctga caccatcgcc
     2341 gccaacgctg gacgcggaga agacggagat gatccgccag cttcagggta ccagtaacga
     2401 ggctcgcgat gccctctaca agcaacagca ggtcatggct catcggcagg cgcttatgct
     2461 tcatgccagt tatgcgaaaa acggcgacgc accgtccttg aagaccgccg ccgccagcgc
     2521 tgtccccatc gtctcgcggc actacaacat gatcatggcg atggctggtg acgcttcgtc
     2581 gccgatgtaa gccggccgcg caatttcgtg tcacgattac ggaggtgacg gcgtcatcgt
     2641 cgtgatctcc gacgcggccg ggcccaaaac ggacggttcg gtgcggctcc ccgggctgca
     2701 cctcgccgtt ttgcgagaga tgctggcgga catctggcgc gcaactggcc ggtcctgact
     2761 ggtggactgt ccggccggga gctgccccaa tggtgtcagt ctcttttcca aggcagttta
     2821 cgccaaggaa aggcgcgcaa cgccctgtcc ggtggggctg ctgcgcagcg cggctgccct
     2881 ggccgaggtt ctgggtcact gctgccacag atggaagtag caaagttcgt ggcgggtctg
     2941 gcccggcctc tggctgtctg agcattggcg gtgtggctgg ccgaaggcgc aagccgttcg
     3001 agatcgcatt gaagcggctt ctgccggaac tcggcctcga ccggctgatc ggagctgcca
     3061 tgttcgcggt cgatggcgat catggcaggc ttcgacgtct atgatatccg gagcctgggc
     3121 gccggccggc ctgggtcaca gcaggtcggg ccgtcaagtc cgtgtagttg agcctagcgc
     3181 tggcaatatc cagagcgcac cgcagcgatt ggcagcgcga cgttcctcga acagcatcgg
     3241 cctcgggatc aaccgtcgaa gctgatcgtt gagcgccgag gatcaggata cgaggttaga
     3301 ccatggctgg cgacaaactc gctaaatacc aggagatgcg cgactttggg gcgacggctg
     3361 agccgtcggg aaaggacgcg aaggtcgttc cctcggaggc gcttcgcttc gttatccaga
     3421 agcacgccgc cagccatctc catttcgatc tgaggctgga atatgaaggc actttccgat
     3481 cctgggcggt gccgaagggg ccttcgcttg atcccaggga tcgccgcatg gccatggagg
     3541 tcgaagacca cccgctcgac tacggcgact tcgagggcac catccccaaa ggccagtacg
     3601 gtggcggaac cgtcatgctg tgggatcggg gtttctgggc gccggagaag ggtttcgaga
     3661 agatcggagc ggcgttggcc aagggcgaac tcaaattcgt catggaaggc gagcggatgc
     3721 atgggtcatg ggtgatcgtg cgcacccgtc gcgacgaccg cggcaaggcg acctggctgt
     3781 tgatcaagca tcgcgacgaa ggcgccgtgg aaggaaacat ctccggtccg accgacgatg
     3841 accgctcggt cgcctcgggt cgaaccatga cggagatcgc gaatggcaag ggcaaggctg
     3901 caaagccctt catgaccgcg aaaggcgccg cggccggatc ggtctggcag agcaatcgcg
     3961 atgataccgc acctgcaggc ttgatcgcgc cgacaaggtc ccgcccgccc gccaaatcca
     4021 aagcagtttc cgagttgccc gacttcgtcg agccacagct ctgcaagtcg ctggagaagc
     4081 cgccgggcgg tgcgggttgg gcgcacgaga tcaaattcga tggctaccgg atgcagcttc
     4141 ggacggccgg cggcaaggcc actctgtcga cgcgtaaagg actggactgg tcggccaggt
     4201 ttccggagat cgtcgctgcg ggaacagcgc tcaaggacgg tatcatagat ggagaagtcg
     4261 tcgcgcttga tcacaccggc gcccccgact tcgccgcact ccaggccgcg attgccg
//
LOCUS       DASVTM010000023         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_694126,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000023 DASVTM010000000
VERSION     DASVTM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694126"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VF633_00535"
     CDS             complement(<1..448)
                     /locus_tag="VF633_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX8469574.1"
                     /translation="MSIGSIPSVGGVEAAIRRASSATGVDFDFLMKTARRESAMNPSA
                     KARTSSAAGLFQFIEQTWLATVKQSGAKHGYGQYADLIHQGGDGRWRVEGSARNVVMD
                     LRFDPQAASSMAAELTASNAAYLRGRTGQEPGAGDLYAAHFLGPAGA"
     gene            616..1347
                     /locus_tag="VF633_00540"
     CDS             616..1347
                     /locus_tag="VF633_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8469575.1"
                     /translation="MMMKIAGGPGLVSPLDYGERKKPLPRTTLIAIGIVALAHVGVGA
                     ALYYQRFELKQAEQLPEPPIIDISMDKPVIPPEPEKPVVQRNPAPNTAIHHTPLPVFP
                     TDTLATEVFPDATPNTGTVIALSNPMPKVDVPGTATEPPRAPPVITHPRWASQPSAAQ
                     MSRAYPERALDDGVSGSASLICSVSAAGALSNCAVAAENPSNRRFGSAALGLTRYFRM
                     QPGAVDGQAIDGARVSFSVRFAAPE"
     gene            complement(1351..2382)
                     /locus_tag="VF633_00545"
     CDS             complement(1351..2382)
                     /locus_tag="VF633_00545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8469576.1"
                     /translation="MPSSSPLLTTDILVIGGGVLGLCTAVELTRRGRDVLVLDPGGAN
                     ASSVAAGMIAPAIESFIDEASPEHARLLREAGRLWAGVPEVRLKPTPAIWRDGGGEQM
                     VAALRVLGFEASLDARGQVLAPDDVQLDPVAALTALKAVLWTPVVEGQAVRIDRVGTG
                     WGVETESGAIFARTVVLATGAAKPLAGLPQGVAALIRLIEPIRGQIGFTAEVLAETVT
                     RGAGGYVVPTEGGSLIGATMEPGRRDLEPDGATGERLTAMAASLVEQEIADEIDWRVG
                     VRGSTPDGLPMAGFSGEPGLYLALAPRRNGWLLGPLVGRIVADKMGGGDISGYDPEHA
                     AALDALRFA"
     gene            complement(2433..>4165)
                     /locus_tag="VF633_00550"
     CDS             complement(2433..>4165)
                     /locus_tag="VF633_00550"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX8469577.1"
                     /translation="DGSEAEKSVIVADLIAKGTLKALDPVKRPGSYYAASDPRDVARV
                     ESRTFICSDDEADAGPTNNWADPIEMRDRLDGLFDGCMVGRTMYVVPFSMGPVGSKIS
                     ALGVEITDSGYVAVSMGIMTRMGKPALDVLGDEGVFVPAVHTLGAPLAPGQSDVPWPC
                     NADKWIVHFPETREIWSYGSGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILKLT
                     NPEGQAKYIAAAFPSACGKTNLAMLQPTLPGWKAETIGDDIAWMRFGDDGRLYAVNPE
                     AGFFGVAPGTSHASNTNAMATLATNTVFTNTALTADNDIWWEGMTKETPEGMTTWKGV
                     AHDASSGEPAAHPNARFASPASQCPTIAPEWEDPKGVPISAILFGGRRASAVPLVTEA
                     FDWEHGVFLGSTVASEGTAAAENAIGELRRDPFAMLPFCGYNMGDYFAHWLALGAKAD
                     ASKLPRIYFVNWFRKNEDGKFVWPGFGENARVLKWISERLDGKARAMDTAVGRVPSPD
                     ALDLAGLDLSNADLHLLLDVDTQVWSEEASLIPGFYEKFGDRLPKALWAQHEALVERL
                     NAEGGLAVSA"
BASE COUNT          722 a         1504 c         1284 g          655 t
ORIGIN      
        1 cggctcccgc gggaccgaga aagtgagcgg cgtacaggtc gcctgcgccg ggttcctgac
       61 ccgtgcgccc gcgcaaatat gcggcgttcg acgcggttag ctccgcggcc atgctcgaag
      121 ccgcctgcgg atcgaagcga agatccatga caacgttgcg cgccgaaccc tcgacacgcc
      181 agcgaccgtc gccgccctga tggatcagat cggcgtactg gccatatcca tgcttggcgc
      241 ccgactgttt gaccgttgct aaccaggttt gctcgatgaa ctggaacagg cccgccgctg
      301 acgaggtcct ggccttcgcc gacgggttca tcgcgctctc gcgacgcgcc gtcttcatca
      361 ggaaatcgaa gtccacgccg gttgccgacg acgcgcgccg aatggccgcc tctacgccgc
      421 caactgatgg aatcgatccg atactcatga tgaccgtgac gctggcggat cgtggttaat
      481 ggaacgttaa tacagcacac tgatcgaaca tcgctcaagt aaactttgat aagcttggct
      541 ttgcccaaaa gccgccacaa gcatggagcg aacttcacac gtgggaggct ggggagcgcc
      601 acgggagtgc ctgccatgat gatgaagatt gccggcggac cgggtctggt cagcccgctg
      661 gactatggcg aacgcaagaa acccctgccc cgaacgacgc tgatcgccat cggcattgtc
      721 gctctggctc acgtcggtgt cggcgccgcg ctctactacc aaaggttcga gctgaagcag
      781 gcggagcagc ttcccgaacc gccgatcatc gatatcagca tggacaagcc ggtcatccca
      841 ccggagcctg aaaagcccgt cgtccagcgg aatcccgcgc cgaatacggc catccaccac
      901 acgccgctgc ctgtgttccc caccgacacc ttggccactg aggtcttccc ggacgcgacg
      961 cccaacacgg gcacggtcat cgccctgagc aacccgatgc ccaaggtgga tgtgcccgga
     1021 acggcgaccg agccgccccg ggcgccgcca gtcatcacgc acccgcgttg ggccagccag
     1081 cccagtgctg cgcagatgag ccgagcctac ccggaacggg ccctggacga cggggtctcg
     1141 ggttcagcct cgctgatctg ctcggtcagc gcagcgggag ccttgagcaa ctgcgcagtc
     1201 gcggcggaga acccgtccaa ccgccgcttc ggctcggcgg cgctcggcct gacgcggtac
     1261 ttccgcatgc agcccggcgc ggtcgatggt caagcgatcg acggcgcccg ggtgagcttc
     1321 agcgtccgct tcgcggcgcc cgagtaaggg tcaggcgaac ctgagagcgt cgagggcggc
     1381 ggcatgctcc gggtcgtatc cggagatgtc gccgcccccc atcttgtccg caacgatacg
     1441 cccgaccagc ggccccagca gccagccgtt ccgacgcggc gccagcgcga ggtacaggcc
     1501 cggctcgccg ctgaacccgg ccatcggcag cccgtccggc gtcgatcccc gcacaccgac
     1561 ccgccagtcg atctcgtctg cgatctcctg ttccacaagg ctcgccgcca tggccgtcaa
     1621 ccgctcgccg gtagccccgt ccggctccag atcacgccgt cccggctcca tcgtcgcccc
     1681 aatcaggctc ccgccctcgg tcggaaccac atagccccct gccccccgcg tcacggtctc
     1741 cgccagcacc tcggcggtga agccgatctg cccccggatc ggctcgatca ggcggatcag
     1801 cgccgcgacg ccttgtggca gtcccgctag cggcttagcc gccccggtcg ccagcaccac
     1861 tgtcctggcg aagatcgcgc cgctctcggt ttcgacgccc cagcctgttc caacccgatc
     1921 gatccgcaca gcttgccctt cgacgaccgg cgtccagagt accgccttga gagctgtaag
     1981 ggcggcgacc ggatccagct gaacatcgtc cggtgcaagc acctgccctc gcgcgtcgag
     2041 cgaggcttcg aaccctaaca cccgaagcgc cgccaccatc tgctcacctc ccccatcacg
     2101 ccagatggcc ggggtgggct tcaaccgcac ctcaggaacg ccggcccaca accgcccagc
     2161 ctcgcgcaac agccgggcgt gttcgggcga ggcctcgtcg atgaaggact cgatcgccgg
     2221 tgcgatcatc cccgctgcaa ccgacgaggc attggcgccg ccgggatcca gcacgagcac
     2281 atcccggcct cggcgcgtca gctcaaccgc cgtgcacaag ccgagcacgc cgcctccgat
     2341 gacgaggatg tcagtagtca ggagaggaga cgatgagggc acaggcagtc cttcgaccgc
     2401 ccgcgcctcc gtttcaagcc gtcaaagcgt cctcaggccg aaacagccag cccaccttcg
     2461 gcattcaacc gttccacaag cgcctcatgc tgggcccaga gcgccttggg cagccggtcg
     2521 ccgaacttct cgtagaagcc tgggatcagc gacgcctcct cgctccagac ctgagtgtcg
     2581 acgtccagca acaggtgcag gtcggcgttc gacaggtcga gacccgcgag gtccagagcg
     2641 tccggcgacg gcacgcgccc cacagccgtg tccatagccc tcgccttgcc gtcgagccgt
     2701 tcggatatcc acttcagcac gcgggcgttt tcgccaaagc cgggccagac gaacttaccg
     2761 tcctcgttct tgcggaacca gttgacgaag tagatgcgcg gcagcttcga ggcgtccgcc
     2821 ttcgccccca aagccagcca gtgcgcgaaa tagtcgccca tgttgtagcc gcagaacggc
     2881 agcatggcga aggggtcgcg acgcagctcg cctatggcgt tctcggcggc agcggtccct
     2941 tccgaagcca cggtcgagcc gaggaacacc ccgtgctccc agtcgaacgc ctcggtcacc
     3001 agaggcacgg ccgacgcacg acgcccgccg aacagaatgg ccgagatcgg cacacccttg
     3061 gggtcctccc actccggcgc aatggtcggg cactgcgagg cgggagaggc gaagcgggcg
     3121 ttcggatggg cagcgggttc gccggaagaa gcgtcatgcg ccacgccctt ccaggtcgtc
     3181 atcccctccg gcgtctcctt cgtcatgcct tcccaccaga tgtcgttgtc ggcggtcagg
     3241 gcggtgttgg tgaagacggt gttggtggcc agcgtggcca tggcgttggt gttcgacgca
     3301 tggctggtgc ccggcgcgac gccgaagaag ccggcctcgg ggttcaccgc atacagccgc
     3361 ccgtcgtcac cgaagcgcat ccaggcgatg tcgtcgccga tggtctcggc cttccagccc
     3421 ggcagcgtcg gctgcagcat ggccaggttg gtcttgccgc aggcgctcgg gaaggcggcg
     3481 gcgatgtact tggcctgacc ctcgggattg gtcagcttga ggatcagcat atgctcggcc
     3541 agccagccct cgtcgcgggc catgaccgac gcgattcgca gcgcgtagca cttcttgccc
     3601 agcagcgcgt tgccgccgta gcctgaccca taggaccaga tctcgcgcgt ctcggggaaa
     3661 tggacgatcc acttgtcggc gttgcagggc cacggcacat ccgactgccc gggagccagc
     3721 ggcgcgccga gcgtatgcac ggccggcacg aacaccccct cgtcgcccag cacgtccagc
     3781 gccggcttgc ccatccgcgt catgatgccc atcgacacgg cgacatagcc gctgtcggtg
     3841 atctcgacgc cgagcgcgct gatcttcgac cccaccggcc ccatcgagaa cggcacgaca
     3901 tacatcgtcc gcccaaccat gcagccgtcg aagaggccgt cgaggcggtc gcgcatctcg
     3961 atcggatcgg cccagttgtt ggtcggcccg gcgtcggctt catcgtccga gcagatgaag
     4021 gtccggctct cgactcgcgc tacgtcgcgc ggatcggagg ccgcatagta gctaccgggg
     4081 cgcttgaccg gatcgagcgc cttcagcgtc cccttggcga tcagatccgc gacgatgacc
     4141 gacttctccg cctccgatcc atcgc
//
LOCUS       DASVTM010000024         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_55541, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000024 DASVTM010000000
VERSION     DASVTM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55541"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="pgk"
                     /locus_tag="VF633_00555"
     CDS             <1..649
                     /gene="pgk"
                     /locus_tag="VF633_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX8469578.1"
                     /translation="GNPDKPVIGIVGGAKVSTKLDLLKNLVGKLDTLAIGGGMANTFL
                     FAQGVDIGGSLAEKDMAETAREIMAEAAAKGCEILLPVDVVVATEVKPGAAARTVKTG
                     ESLSADDKILDAGPETVARLNAAIDASKTLIWNGPLGVFEVPPFDAATVAVAKHVAEK
                     TKAGALIAVAGGGDTVSAVNHAGVADDLTFVSTAGGAFLEWMEGKPLPGVEALSV"
     gene            776..1861
                     /gene="fba"
                     /locus_tag="VF633_00560"
     CDS             776..1861
                     /gene="fba"
                     /locus_tag="VF633_00560"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505152.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX8469579.1"
                     /translation="MARITLRQLLDHAAEHSYGLPAYNINNMEQGLAIMEAAEEVNAP
                     VIIQASRGARNYANDIVLAKLIDALAELYPHIPVCMHQDHGNGPATCATAIQYGFTSV
                     MMDGSLEEDAKTPASYEYNVEVTRRVTQMAHSCGVSVEGELGVLGSLETGMGEAEDGH
                     GFEGKLDHSALLTDPDQAVDFVKATQVDALAIAMGTSHGAYKFSRKPDGEVLAMNVIE
                     EIHRRLPDTHLVMHGSSSVPQDLQDIINEYGGKMPQTWGVPVEEIQRGIKHGVRKINI
                     DTDNRMAMTGAVRKLLMEKPGEFDPRAWMKPAKEAMRKVCKQRFQEFGCEGMASSIKP
                     LSTAQMAIRYASGELNPHYGKAASQAA"
     gene            1966..>2220
                     /locus_tag="VF633_00565"
     CDS             1966..>2220
                     /locus_tag="VF633_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8469580.1"
                     /translation="MSENAQPGEDFEDGGDTLTVTLGPDSRALRLDKALADAVPTLSR
                     ARIQALLDAGAIYRDETRLTGGSGKATPGEYLIVLPPLISA"
BASE COUNT          437 a          730 c          698 g          355 t
ORIGIN      
        1 gggcaatccg gacaagccgg tgatcgggat cgtggggggg gcgaaggttt cgaccaagct
       61 cgatcttctc aagaacctgg tcggcaagct cgatacgctc gcgattggcg gcggcatggc
      121 caacaccttc ctgttcgcac agggcgtgga tatcggcggc tcgctggccg agaaggacat
      181 ggccgaaacc gcccgtgaga tcatggcgga ggcagcagcc aagggctgcg aaatcctgct
      241 gcccgtcgat gtcgtcgtgg cgaccgaggt taagccgggc gctgctgctc gcacggtcaa
      301 gactggtgaa tcgctgtccg ccgacgacaa gattctcgac gccggccccg agacggttgc
      361 tcgcctcaac gcggcgatcg atgcatcgaa gaccctgatc tggaacggcc ccttgggcgt
      421 gttcgaagtg ccgccgttcg atgccgccac ggtcgccgtc gcaaagcatg tcgctgaaaa
      481 gaccaaggcc ggcgccctga tcgcggtcgc aggcggcggc gacaccgttt cagccgttaa
      541 tcacgcaggc gtggccgacg acctgacctt cgtctcaacg gccggcggcg ccttcctcga
      601 atggatggag ggcaagcccc tgcccggcgt tgaggctcta agcgtctagg gtgccgcgca
      661 acgtcgggtc gcaaggacgg cgcccgacgg aaaccctgtt acacggcgtg actttgttgc
      721 ggtgcggcgc tagtctttct gcaaataaaa cagactgaca ttcagggatc aaatcatggc
      781 gcgcatcacg ctgcgacagc tcctcgacca cgcggccgag cacagctacg ggcttccagc
      841 ctacaatatc aacaatatgg aacagggtct ggcgatcatg gaggcggccg aagaggtcaa
      901 tgcccccgtc atcatccaag cctcgcgcgg cgctcgcaac tacgccaatg acatcgtcct
      961 cgccaagctg atcgacgctc tggccgagct ctacccgcac atccccgtct gcatgcacca
     1021 ggaccacggc aacggcccgg cgacctgcgc caccgccatc cagtacggct tcacctcggt
     1081 gatgatggac ggttcgctgg aagaagacgc caagaccccc gccagctacg aatacaacgt
     1141 cgaggtcacg cgccgcgtga cccagatggc ccacagctgc ggcgtgtcgg tcgaaggtga
     1201 gctaggtgtt ctcggctcgc ttgaaactgg catgggcgag gccgaagacg gccacggctt
     1261 cgaaggcaag ctcgatcatt ccgccctgct gaccgaccct gaccaagccg tcgacttcgt
     1321 caaggccact caggtcgatg cacttgccat cgctatgggc acatcccacg gcgcctataa
     1381 gttcagccgc aagccggacg gcgaggtcct cgccatgaac gtgatcgagg aaatccaccg
     1441 ccgcctgccc gacacccatc tggtaatgca cggctcgtcg tcggtgccgc aggacctgca
     1501 ggacatcatc aacgagtacg gcggcaagat gccccagacc tggggcgtgc cggtcgagga
     1561 aatccagcgc ggcatcaagc atggcgtgcg caagatcaac atcgataccg acaaccgcat
     1621 ggcgatgacc ggcgcagtcc gtaagctgct gatggaaaag ccgggcgagt tcgatccccg
     1681 cgcctggatg aagccggcca aggaagcgat gcgcaaggtc tgcaagcagc gcttccagga
     1741 gttcggctgc gagggcatgg ccagcagcat caagccgctc tccaccgccc agatggccat
     1801 ccgctacgcc tctggcgagc tcaacccgca ctacggcaag gccgcctccc aggcagcttg
     1861 atatggcagg ggagcgggcc gctggggtgc ggttcggtcc cttgaccacg ctcgaaggat
     1921 cgcccggcct gtcgcggccg gacgaaaccc cctacatccc ggctgatgtc agagaacgcg
     1981 cagccaggtg aggatttcga ggacggcggc gacacgctga ccgtcaccct ggggccagac
     2041 agccgggctt tgcgcctcga caaggcgctc gcggacgccg ttccgaccct ttcgcgcgcc
     2101 cgtattcagg ccttgctcga cgcgggagcg atctaccgtg acgagacacg cctgactggg
     2161 ggctccggca aggctacgcc cggcgaatac ctgatcgtgt tgccgccgtt gatttcagcg
//
LOCUS       DASVTM010000025         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_958790,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000025 DASVTM010000000
VERSION     DASVTM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958790"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF633_00570"
     CDS             <1..337
                     /locus_tag="VF633_00570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170187.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX8469581.1"
                     /translation="GDPVLVDDNKVVPPRRGEMKRSMEALIHHFKLYTEGFRTPPGEV
                     YAAVEAPKGEFGVYLVSDGTNRPYRCKVSSPGFRHLQAMDWMNRGHMLADVSAILGSL
                     DIVFGEVDR"
     gene            334..525
                     /locus_tag="VF633_00575"
     CDS             334..525
                     /locus_tag="VF633_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469582.1"
                     /translation="MSDAPISNLIAQGWEIAGYSATDASGTAYQHSVLLRRQGRHKIV
                     VLRKKILGDGIVVDSELDV"
     gene            525..1205
                     /gene="nuoE"
                     /locus_tag="VF633_00580"
     CDS             525..1205
                     /gene="nuoE"
                     /locus_tag="VF633_00580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX8469583.1"
                     /translation="MSVRRLAKVQPASFAFSDATMEKAQWWIAKYPADRRQSAVTPIL
                     WLVQKQEGWVSEPALRAIAELLGMAYIRVLEVATFYTMFMLEPVGSAALIQVCGTTPC
                     MLRGAGELMKVCKSRIGPKQTLSADGKFYWEEVECLGACVNAPMAMVNDYYHEDLTAA
                     DMNAIIDGFAAGAPLERGPRVERVNSAPEGGALTLLDPKLYDGSSAVKITKLPNSDPA
                     PVPEKAPA"
     gene            1202..1471
                     /locus_tag="VF633_00585"
     CDS             1202..1471
                     /locus_tag="VF633_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469584.1"
                     /translation="MSLPDLNTDEGRAAYRKELRKVVMPIRWTGLALIILGGVLAMMA
                     RTHTLGLDNGVLPFAYGALAFGWVLFLVATVVRTRHHRRRLAEGL"
     gene            1478..2791
                     /gene="nuoF"
                     /locus_tag="VF633_00590"
     CDS             1478..2791
                     /gene="nuoF"
                     /locus_tag="VF633_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX8469585.1"
                     /translation="MVGILEDKDRIFTNLYGLQDWGLEGAKNRGCWNATKDMLDMGRD
                     WLITNVKNSGLRGRGGAGFGTGLKWSFMPKEVKDRPHYLVVNADESEPGTCKDREIMR
                     HDPHLLIEGCLIASFSMQAHACYIYLRGEYILERERMEAAVKQAYEANLIGKNNVHGW
                     DFDVYIHHGAGAYICGEETALLESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIA
                     VVGTILRRGADWFAGFGRANNTGTKLMSISGHVNRPCNVEEAMSIPLRQLLEDHCGGV
                     RGGWGNLKAIIPGGVSVPLITREMSETALMDFDSLREMKSGLGTAAVIVMDNDTDLVK
                     AIARISYFYKHESCGQCTPCREGTGWMWRVLERMAIGEADPSEIDLLLDVASQVEGHT
                     ICALGDAAAWPVQGLIRHFRHEIEDRIQQYRTSRANFAGHAIAAE"
     gene            2791..3288
                     /locus_tag="VF633_00595"
     CDS             2791..3288
                     /locus_tag="VF633_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469586.1"
                     /translation="MRAAIIIAGGLLGLAACGQPAEKAAPTPAEKVSATPVVSDAATT
                     PVGPLSAADLRRVCRAGLAAIHGQEPTAIEITAVEGDVVNAQWRAPVDGGQMKAQCRT
                     DGELLTWKPLELPDTADIRWMNQSGDPVNKFAIKGDVVTVTQTYPDGTNQQSDLTVPA
                     TKEAA"
BASE COUNT          599 a         1105 c         1048 g          581 t
ORIGIN      
        1 cggcgacccg gtgctggtcg acgacaacaa ggtcgtcccc ccgcgccgcg gcgagatgaa
       61 gcgctcgatg gaagccttga tccaccactt caagctctac accgaaggct tccgcacccc
      121 tccgggtgag gtctatgccg ccgtcgaagc ccccaagggt gagttcggcg tgtatcttgt
      181 cagcgacggc acgaaccgtc cgtatcgttg caaggtctct tctcccggct tccgtcacct
      241 tcaggccatg gactggatga accgcggtca catgctggcc gacgtctccg ccatcctcgg
      301 ctcgctcgac attgtgttcg gcgaggtcga ccgatgagtg atgccccgat cagcaatctg
      361 attgcccagg gttgggaaat cgcgggctat tccgcgactg acgcctccgg gaccgcctat
      421 cagcatagtg tcctgctgcg ccgtcagggc cggcacaaga tcgtcgtgct gcgtaagaaa
      481 atcctcggcg acggcatcgt cgtcgactcg gaattggacg tctgatgtcg gttcgtcgcc
      541 tcgccaaagt ccagcctgcc tcgttcgcgt tctcggatgc gacgatggaa aaggcccagt
      601 ggtggatcgc caaatatccc gctgaccgtc gtcaatcggc cgtgaccccg atcctgtggc
      661 tggtgcagaa gcaggagggc tgggtctccg agcccgcgct gcgcgcaatc gccgagctgt
      721 tgggcatggc ctacatccgg gtgctggagg tcgcgacctt ctacaccatg ttcatgctgg
      781 agccggtcgg ctcggccgcc ctgatccagg tctgcggcac cacgccctgc atgctgcgcg
      841 gcgcgggtga gctgatgaag gtctgcaaga gccgcatcgg cccgaaacag accctctcgg
      901 ccgacggcaa gttctactgg gaagaggtcg agtgcctcgg cgcctgcgtc aacgcgccga
      961 tggccatggt caacgactac taccacgaag acctgacggc ggccgacatg aacgccatca
     1021 tcgacggctt cgcggcaggc gctccgctgg agcgtggccc gcgcgtggag cgcgtcaact
     1081 ccgctcccga gggcggcgcg ttgacgctgc tcgatccgaa actctacgac ggttcgtccg
     1141 ccgtgaagat caccaaactg ccgaactccg accctgcgcc ggttcctgaa aaggccccgg
     1201 catgagcctt cccgacctga acaccgacga aggccgcgcc gcctaccgca aggaactgcg
     1261 caaggtggtc atgccgatcc gctggacggg tctggcgctg atcattctcg gtggcgttct
     1321 cgccatgatg gcccgcaccc atacgctggg tctcgacaat ggcgttctgc cgttcgccta
     1381 tggcgccttg gccttcgggt gggtgctgtt tctggtcgcg acggtcgttc gcacccgcca
     1441 tcacagacgc cgtttggcgg aaggacttta ggacgccatg gtcggcattc tcgaagacaa
     1501 ggatcgcatc ttcaccaacc tttacgggct ccaggactgg ggcctcgaag gcgcgaagaa
     1561 ccgcggctgc tggaacgcga ccaaggacat gctggacatg ggccgcgact ggctcatcac
     1621 caacgtcaag aactcggggc tccgtggacg cggcggcgcg ggcttcggca ccggcctgaa
     1681 atggtcgttc atgcccaagg aagtgaagga ccgtcctcac tatctggtcg tcaacgccga
     1741 tgagtcggag cccggcacct gcaaggaccg ggagatcatg cgccatgatc cccacctgct
     1801 gatcgaaggc tgtctaatcg cctcgttctc gatgcaggcc cacgcctgct acatctacct
     1861 gcgcggcgaa tacatcctcg agcgcgagcg gatggaggct gcggtcaagc aggcctatga
     1921 ggccaacctc atcggcaaga acaacgtcca cggctgggac ttcgacgtct acatccacca
     1981 cggcgccggc gcctacatct gcggcgaaga aaccgccctg ctggaaagcc tcgagggcaa
     2041 gaagggccag cctcgcctga agccgccgtt cccggccggc gcaggcctct acggctgccc
     2101 gaccacggtc aacaatgtcg agtcgatcgc cgtcgtcggc acgatcctgc gtcgcggggc
     2161 cgactggttc gcgggcttcg gtcgcgccaa caacaccggc accaagctga tgtcgatctc
     2221 gggccacgtg aaccgcccgt gcaatgtcga agaagcgatg tcgatcccgc tgcgccagtt
     2281 gctggaagac cattgcggcg gcgttcgcgg cggctggggc aacctcaagg ccatcattcc
     2341 cggcggcgtc tccgtcccgt tgatcacgcg tgagatgtcc gaaaccgcgc tgatggactt
     2401 cgactccttg cgcgaaatga agtcgggtct cggcaccgcc gccgtgatcg tcatggacaa
     2461 tgacaccgac ctcgtgaagg cgatcgcccg catcagttat ttctacaagc atgagagctg
     2521 cggccagtgc acgccttgcc gcgaaggcac cggctggatg tggcgcgtgc tcgaacgcat
     2581 ggcgatcggc gaggcggatc cgtccgagat cgacctgctg ctcgacgtcg cctctcaagt
     2641 cgaaggccac accatctgcg ctcttggcga cgctgcggcc tggcccgttc aaggcctgat
     2701 ccggcatttc cgccacgaga tcgaggaccg catccagcag taccgcacca gccgcgcaaa
     2761 cttcgccggc cacgcgatcg cggccgaata atgcgggcag cgatcatcat cgctggagga
     2821 cttctgggtc tggccgcttg cggtcagcct gcggagaagg ctgcgccgac accggctgag
     2881 aaggtctcgg ccacgccggt cgtgtccgac gccgcgacga cgccggtcgg tccgctgtcg
     2941 gcggccgatc tgcgccgcgt ctgccgtgcg ggcctcgccg ccatccatgg tcaggagccg
     3001 acggcgatcg agatcaccgc cgtcgaaggt gatgtcgtga acgctcagtg gcgcgctccg
     3061 gtcgacggcg gccagatgaa ggcccagtgc cgcaccgacg gcgagcttct gacgtggaag
     3121 ccgctcgaac tgcccgatac cgccgacatc cgctggatga accagtcggg tgatccggtg
     3181 aacaaattcg ccatcaaggg cgacgtcgtg acggtcaccc agacctatcc cgacggcacg
     3241 aaccaacagt cggacctgac ggttccggca actaaagagg ccgcgtgatg aagcgacctt
     3301 tagcgatcat ggtctggtcg gtcgtctggc tgg
//
LOCUS       DASVTM010000026         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_321130,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000026 DASVTM010000000
VERSION     DASVTM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321130"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VF633_00600"
     CDS             <1..1325
                     /locus_tag="VF633_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX8469587.1"
                     /translation="EQFYTAAKAAADALVAGQHPRGGWNYVIDTAGEESLKDWYAKIG
                     GNAWRLEEYHRYYGNATFDDAGTAEASQLLLRVYLEKRERKYRAPLDKAIQFVLDAQY
                     ANGGWPQRFPFVEGGGIHGHADYTPYITFNDDVAGENIEFLLYAHQALGSNDARLVDA
                     IERGMDIYVETQQPMPQPAWGLQHFPDTLKPAPARTIEPQAFATHTTGTNIRSMIGFY
                     KLTGDRKFLARLPEALDWLDQVKTPQNLIAPGRTHPTFILEGSNTPIYIHRKGSNIAN
                     GHYYADTNPNNVIIHYGSFRSVDTAAIRREYEAAAAMDPAALIAASPLKAKAGSLKLP
                     KYFTLRDVSIWDMSTDARREKTPAPEAVAAVLSSLTDNGCWLTPLTTTSNPYNGPSPA
                     AVTGGEYQTTRVGDLWDVSPYNTDFPVEGISTAVFIKNMGDLIEALQA"
     gene            1383..3080
                     /locus_tag="VF633_00605"
     CDS             1383..3080
                     /locus_tag="VF633_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX8469588.1"
                     /translation="MDASGRLEIGAGFATALGPRADNQDFGAVHLGTPSEQALHGIVA
                     VVADGVSGSKGGRIAAELATRSFIDGYLSKSPLGGIAAAGVASLLGFNRWLHAKGRTD
                     PEMKGAATTFTALVLRGREATVLHVGDSRAWHYRDGVLTLLTEDHVLPQPGLSHVLYR
                     AVGIEADVRLDVRPVPLKPFDRFLLTSDGVHGVVDEDALKRLLGRRASPDADAQAIVD
                     QATLEGATDNATAIVIDVIRLGPLDSEAIGIEAEGLPVLPPPDTGDNVDGFRLERLLS
                     DGKYTRVILARDGADPTPIVLKFPKPALLSEHGARLAFLRESFIGRRIDSPFVGKTLT
                     LDPGRQSRLYIAQPFYEGRTLHARLASDGPFDIQPGVTEAIRLSRAVAALHRQGIAHR
                     DIKPDNVILEKNGGLKLIDLGVARLPRVEEFTESEAPGTAGYMAPELFDGNPGDALTD
                     QYALGVTLYRLFAKDYPRGQVELPQRPKFEKPVPPTQLRPDMPAWLEAAILRAIVVDR
                     DDRFADVEEMIHVLETGSAVAVLPPQPPSLIERDPVRFWQGVCILLVIALMISLATR"
     gene            complement(3309..>4201)
                     /locus_tag="VF633_00610"
     CDS             complement(3309..>4201)
                     /locus_tag="VF633_00610"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX8469589.1"
                     /translation="DWLQKSELETGTTLRAMTIQEAGGTGRAIEAGIEIVRELVDEAS
                     KVERQTVPASFLTVGLQCGGSDGWSGVTANPALGKAVDLLVAHGGSAMLSETPEIWGA
                     EHLLLRRAASQDVADKLNARLAWWRSYADKHGMELNNNPSPGNLKGGLTTILEKSLGA
                     VAKSGSTPLTDVIGYAEPLKTQGLTFMDSPGYDPCSATGQIASGANLIVFTTGRGSVF
                     GAKPAPSIKVASNAKLASWMDEDMDIDASPVLTGTPMADVGAEIFERMLAVASGEQSK
                     SEALGIGDNEFVPWAVGAYL"
BASE COUNT          728 a         1461 c         1341 g          671 t
ORIGIN      
        1 acgaacagtt ctacaccgcc gccaaggccg cagccgacgc cctcgtcgca ggccagcacc
       61 cgcgcggcgg ctggaactac gtcatcgaca ccgccggcga ggagagcctc aaggactggt
      121 acgccaagat cggcggcaac gcctggcggc tcgaggaata tcaccggtac tacggcaatg
      181 cgacgtttga cgacgcgggc acggccgagg cttcgcaact gctgctgcgg gtctatctcg
      241 agaagcgtga gcgcaaatat cgcgcgccgc tcgacaaggc gatccagttc gttctcgacg
      301 cccagtacgc caacggcggc tggccgcagc gcttcccgtt cgtcgagggc ggcggcattc
      361 acggccacgc cgattacacg ccctacatca ccttcaacga tgatgtagcc ggcgagaaca
      421 tcgagttcct gctgtatgcg catcaggcgt tgggctccaa cgacgctcgt ctggtcgacg
      481 ccatcgagcg cggcatggac atctatgtgg agacccagca gccgatgccg cagccagcct
      541 ggggcctgca gcatttcccc gacacgctga agcccgcccc ggcccggacg atcgagccgc
      601 aagccttcgc gacccacacc accggcacga acatccgctc gatgatcggc ttctacaaac
      661 tgaccggcga ccggaagttc ctggcccgcc tgcctgaggc gctggactgg ctggatcagg
      721 tcaagacgcc gcagaacctg attgcgcccg gccggacgca tccgaccttc atcctcgaag
      781 gctcgaacac gccgatctat atccaccgca agggctcgaa tatcgccaac ggccactact
      841 atgccgacac gaacccgaac aatgtgatca tccactacgg ttcgttccgc agtgtcgaca
      901 ccgccgccat ccgtcgcgaa tacgaggccg ccgccgccat ggatccggct gcgctgatcg
      961 ccgcctcgcc gctgaaggcc aaggccggtt cgctgaagct gccaaaatac ttcaccctgc
     1021 gcgacgtcag catctgggac atgtcgaccg acgcccgccg cgagaagacc cctgcgcccg
     1081 aggctgtcgc cgccgtcctg tccagcctga ccgacaacgg ctgctggctc acgcctctga
     1141 ccaccacgtc caatccgtac aatggcccct ccccggctgc tgtgacgggc ggcgagtatc
     1201 agacgacccg tgtcggcgac ctgtgggacg tctcgcccta caacaccgac ttcccggtcg
     1261 aaggcatctc cacagccgtc ttcatcaaga acatgggcga cctgatcgaa gcgcttcaag
     1321 cctgacggga cttgacggcg cgtccgcttg cctccccatg atcggataac ggggatgggt
     1381 tgatggacgc gtcgggcagg ctggagatcg gcgcgggatt cgctacggcg ctgggccccc
     1441 gcgcggacaa tcaggacttc ggcgccgtcc atctcggcac gccgtccgaa caggcgctgc
     1501 acggcatcgt ggctgttgtc gccgacgggg tcagcggctc gaagggcggg aggatcgccg
     1561 ccgaactggc cacccgcagc ttcatcgacg gctatctgtc gaaaagcccg ctcggtggca
     1621 tcgccgccgc aggcgtcgcc tccctgctcg gcttcaaccg ctggctccac gccaagggtc
     1681 gcaccgatcc cgagatgaag ggggcggcga cgacgttcac ggcgctggtc ttgcggggcc
     1741 gcgaggcgac cgtgctgcac gtcggcgaca gccgcgcatg gcattaccgc gacggcgtcc
     1801 tgaccctgtt gaccgaggat cacgtcctgc cgcagccggg gctaagccat gtgctctacc
     1861 gcgccgtggg gatcgaggcc gacgtgcgtc tcgatgtccg gcccgtgccg ctgaagcctt
     1921 tcgaccgttt cctgctgacc agcgacggcg tgcatggcgt ggtcgatgaa gacgcgctca
     1981 aacgcttgct cggccgccgc gcgtcccccg atgcagacgc ccaggccatc gtcgatcagg
     2041 cgacgctgga aggcgcgacc gataacgcga ccgccatcgt catcgacgtg atccgtctgg
     2101 gcccgctcga cagcgaggcg atcgggattg aggccgaggg cctgccggtc ctgccgccac
     2161 cggatacggg cgacaacgtg gacggcttcc ggctggagcg gctgctgtcg gacggcaaat
     2221 acacgcgggt cattctggcc agggacggcg ctgatccaac gcccatcgtc ctgaaattcc
     2281 ccaagcccgc cctgctcagc gagcacggcg cgcggctggc gttcctgcgc gagagcttca
     2341 tcggccgtcg cattgacagc ccgttcgtcg gcaagaccct gacgctggat cccggccgac
     2401 agagccgcct ctatatcgcc cagcccttct atgagggtcg gaccctgcat gcccgactgg
     2461 ccagcgacgg accattcgac attcagccgg gcgtcaccga ggccatccgc ctgtcccgcg
     2521 ccgtcgccgc cctgcaccgc caaggcatcg cccaccgcga catcaagcca gacaatgtga
     2581 tcctcgagaa gaacgggggc ctgaagctga tcgacctcgg cgtagcccgc ctgccccgcg
     2641 tcgaggagtt caccgaaagc gaggcccccg gcaccgccgg atacatggcg cccgaactgt
     2701 ttgacggaaa tcccggcgac gccctgaccg accaatatgc gctgggggtc accctctatc
     2761 gcctgttcgc caaggactat ccgcgcggtc aggtcgagct gccgcagcgg ccgaagttcg
     2821 agaaaccggt cccgccgacg cagctccgtc ccgacatgcc tgcatggttg gaggccgcca
     2881 tcctgcgcgc catcgtcgtc gatcgcgacg accggttcgc ggacgtcgag gaaatgatcc
     2941 acgtgctgga gaccggcagc gccgtcgccg tcctcccgcc tcagccgccg tccttgatcg
     3001 agcgcgaccc ggtccggttc tggcagggcg tctgcatcct gttggtcatc gccttgatga
     3061 tctcgctggc gacgcggtag gacctacagc ctcggtcgag gtggcggcgg caggggcgcg
     3121 ggcggaggcg atggcggcgc gctgtgcgga gggcgcggcg gataataccg cgccggatag
     3181 tgtgattggc aagcgctgag cagcccggcg cccgccacca ctgtgccgat caacttggag
     3241 aggtcggcaa gatggcgctg ggtcatgccg atcaaagaac atcgataccc gatggcgtca
     3301 acgcttgctt agagataggc cccgacagcc cagggaacga actcgttgtc gccgatgccg
     3361 agcgcctccg acttggattg ctcacccgag gccacggcca gcatccgttc gaagatctcc
     3421 gcgccgacat ccgccatcgg cgtgccggtc aggaccggcg aggcgtcgat gtccatgtcc
     3481 tcgtccatcc acgacgccag tttggcgttg gacgcaacct tgatcgacgg ggcgggcttg
     3541 gcgccgaaca ccgagccacg gcccgtggtg aagacgatca gattggcgcc cgaggcgatc
     3601 tggcctgttg ccgaacaggg gtcgtagccg ggactgtcca tgaaggtcag gccctgcgtc
     3661 ttcagcggct cggcatagcc gatgacgtcg gtcagcgggg tcgaacccga cttggccacc
     3721 gcgcccagcg acttctcgag gatggtggtc aggccgccct tgaggttgcc cggggacggg
     3781 ttgttgttga gctccatgcc atgcttgtcg gcataggaac gccaccaggc gagacgcgcg
     3841 ttgagcttgt cggccacgtc ctgcgaggcc gcgcgacgca gcagcagatg ttcggcgccc
     3901 cagatctcgg gcgtttccga cagcatggcc gaaccgccgt gggcgaccag cagatcgacg
     3961 gccttgccca aagccgggtt ggccgtgacg ccggaccagc cgtccgaacc gccgcattgc
     4021 aggccgacgg tcaggaatga agccgggacc gtctggcgct cgactttcga ggcttcgtcg
     4081 accagttcgc ggacgatctc gatcccggcc tcgatggcgc gaccggtgcc gccagcctct
     4141 tgaatcgtca tggcgcgcag ggtcgtgccg gtttcgagtt ccgacttctg caaccagtcg
     4201 g
//
LOCUS       DASVTM010000027         4972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_694802,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000027 DASVTM010000000
VERSION     DASVTM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694802"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VF633_00615"
     CDS             complement(<1..941)
                     /locus_tag="VF633_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX8469590.1"
                     /translation="MASRLTPRPDTLEAALAKVFDDRNTRASWFALTGGESLFAAGEE
                     ADSLYLLRSGRLGVFRREEGQPPYFVGVIRPGEPVGEMALIAGTSHTSDVVALRDSEI
                     LALPRDAFFDAVKTMPEVMIELSRLMLHRARERTTGATEPSVFGFISARPRPIRAFVE
                     RIAAAIEAQGSTCQIIDHSALSSASEWFSRVEDSHDYVLYVAEHDEPNWASLCARQVD
                     RLFIVGNPLLAPPSNVPTRNGGMVAGKTDRSGHQFTDLILLRDPRMARPANTAVWLDA
                     LEPGRWFHCVEGEAADAERMARVLTGASVGLVLSGGGA"
     gene            1117..2631
                     /gene="serS"
                     /locus_tag="VF633_00620"
     CDS             1117..2631
                     /gene="serS"
                     /locus_tag="VF633_00620"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX8469591.1"
                     /translation="MHDIRALRETPDAYVAGWSSRGVVDAQGVVDQILALDLELRAAQ
                     TAGQDALAKRNAASKLIGAAMGRKDMAEADRLKGEVDSLKGEIAGFEATEKDVGGRLR
                     DMLAREQNLAADGVPDGEDESGNVEVSTWGEPRRTGPAKDHSDLGEALGMLDFEAAAR
                     MSGARFAVIKGPLARLERAIGQFMLDLQTGEHGYLEVNPPFLVKDEAMFGTGQLPKFE
                     IDLFRTDQIDFAAYDQALREAAYGGFQQDFDRLTKLREEMVSTLDPEVFEIAWLKVLE
                     DANSEARSRDLPPVLERLQRGEFSDWRYLIPTAEVSLTNLVREQILTEDEIATPMRLT
                     ALTPCFRAEAGSAGRDTRGLIRQHQFHKVEMVSIARPEDSDAEHERMTNCAETVLQKL
                     ELPYRRMLLCKGDMGFTAKKTFDLEVWLPSQATYREISSCSNCGDFQARRMDARFKRA
                     GEKKTEFVHTLNGSGLAVGRTLVAIMENYQDEDGRIAVPAALQPYMGGLTHVGV"
     gene            2635..3039
                     /locus_tag="VF633_00625"
     CDS             2635..3039
                     /locus_tag="VF633_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469592.1"
                     /translation="MTRIALAPALIATALIAAVTAGAASAQIKPPIDPSATTFAGWVR
                     VSHGEFQLYETQRQISEPFSRPCISGALPRNLQRASGDISGQQVTFTGKAVAWAERGT
                     RTTLNHQGSRIVNECGGDYVIAAESVRVLRAG"
     gene            3036..3866
                     /gene="surE"
                     /locus_tag="VF633_00630"
     CDS             3036..3866
                     /gene="surE"
                     /locus_tag="VF633_00630"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX8469593.1"
                     /translation="MRILLTNDDGIEAEGLASLERIARALSDPASSSPTGGRPQENEV
                     WIVAPATEQSGKGRGITLTEPLRVNKLGDKRFSVTGTPTDCVVLAFDLMDAKPDLVLS
                     GVNRGHNVGEDCSYSGTVAGALQGMALGVRSIALSQSLDRFHDDVTAHWETAETYAPA
                     IIATLLAQDWQPGVVMNLNFPARPPEQVKEVEVTVQGFRQIGEMHPVKRTDLRGRDYY
                     WMSFRGAKQDHAPGTDLRAMDDGKISVTPLHIDLTHKASVHDLKGVLGGVPPKAIAAE
                     "
     gene            3863..4507
                     /locus_tag="VF633_00635"
     CDS             3863..4507
                     /locus_tag="VF633_00635"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX8469594.1"
                     /translation="MNLKDDRAGRLILSLRQQGVTDPRVLGAMESIDRAVFVHEKFLD
                     QAWEDQALPIDCAQTISQPYIVGLMTQALDVQPRHRVLEIGTGSGYQCAVLSRLARYV
                     YSVERYRSLMVEAEGKLKLLGIDNVITQHSDGGLGWIQQAPFDRIMVTAASPTEPTEL
                     LKQLKPGGVLVCPVGRTSVQMLHRYTGQPDGSFHRESLREVRFVPLVEGTAKEG"
BASE COUNT          886 a         1582 c         1680 g          824 t
ORIGIN      
        1 gcgcctccgc ctgagagcac caggcccacg gaggcgccag tcagcacccg ggccattcgt
       61 tcggcatctg cggcttcgcc ctcgacgcag tggaaccagc gcccgggctc gagcgcatcg
      121 agccagacgg ccgtgttggc cggccgggcc atgcgggggt cgcgcagcag gatcagatcg
      181 gtgaactgat ggccgctgcg gtcggtcttc ccggcgacca tgccgccgtt tcgcgtgggt
      241 acgttgctcg gcggcgcgag caacgggtta ccgacaataa acagtcgatc gacctgacgc
      301 gcgcacaggg aggcccagtt gggctcgtcg tgctcggcaa catagaggac gtagtcgtgg
      361 ctgtcctcga cccgtgagaa ccactccgat gccgacgaca gggcggaatg gtcgatgatc
      421 tgacaggtag acccttgcgc ctcgatggcg gccgcgatgc gctcgacgaa ggcgcggatg
      481 ggccggggac gggcggagat gaagccgaac acgctgggct cggtggcgcc ggtcgttcgc
      541 tcccgcgccc ggtgcagcat cagccgcgac agctcgatca tcacctcggg catggtcttc
      601 acggcgtcga agaaggcgtc gcggggcagg gcgaggatct cggaatccct caacgccacg
      661 acatccgaag tgtgcgaggt gcctgcgatc agcgccatct ctccgacagg ctcgcccggg
      721 cggatgactc ccacgaagta aggcggctgg ccttcctctc gccggaacac gccgagccgg
      781 cctgatcgca gcaggtacag gctgtcggcc tcttcgccgg cggcgaacag cgactccccg
      841 ccggtgaggg cgaaccagct cgcacgcgta ttgcgatcgt cgaacacctt ggccagagcg
      901 gcttcgagcg tatcgggtcg cggggttaga cgagaggcca tggaaccgga tatgggagtt
      961 gagtctgcgg tgacgctagg cccgggaagc gtgaacgtcg atggggccat tctgatcctt
     1021 ctccccgcag tggagaacga cgggcagcgt tgcccgactc cgttgggggc ggtaaggcgt
     1081 acgccttctg aaacgccgac ctgaaacgac gacgccatgc atgatatccg agcccttcgc
     1141 gagacaccgg acgcctatgt ggccggctgg tcgtcgcgtg gcgtcgtgga tgcgcaaggg
     1201 gtcgtcgatc agatcctggc tctcgacctc gaactgcgag ccgcacagac ggcggggcag
     1261 gatgcgctgg ccaagcgcaa tgcggcgtcc aagctgatcg gcgccgccat gggccgcaag
     1321 gatatggccg aggctgaccg gctgaagggc gaggtcgaca gcctgaaggg cgagatcgcc
     1381 ggcttcgagg cgaccgagaa ggatgtcggc gggcgtctgc gcgacatgct ggcgcgcgag
     1441 cagaacctgg ccgccgacgg cgtgccggac ggcgaggacg agagcggcaa cgtcgaggtg
     1501 tcgacctggg gcgagccgcg ccgcaccggt ccagccaagg accactccga cctcggcgaa
     1561 gcgctgggga tgctggactt cgaggccgcc gcgcggatgt cgggggctcg cttcgccgtg
     1621 atcaagggtc cgttggcgag actggaacgc gccatcggcc agttcatgct ggacctgcag
     1681 accggcgagc atggctatct ggaagtaaac ccgccgttcc tcgtcaagga tgaggcgatg
     1741 ttcgggaccg ggcagttgcc gaaatttgaa atcgatctgt tccgaaccga ccagattgat
     1801 ttcgcagcgt acgaccaagc tcttcgagaa gctgcatatg gcggttttca gcaagacttt
     1861 gatcggctga cgaagctgcg cgaagaaatg gtatccacgt tggacccaga ggtctttgag
     1921 atcgcttggc tgaaagtttt ggaggacgca aactccgaag cgcgctcaag ggatctgcct
     1981 ccagtacttg agcggctcca gcgcggagag ttctctgatt ggcgctacct catccccacc
     2041 gccgaagttt ccctgaccaa tctcgtacgt gagcaaattc tcaccgagga cgagatcgcc
     2101 acgcccatgc ggctgaccgc cctgacgccc tgtttccggg ccgaggctgg gtcggcgggg
     2161 cgcgatacgc gcggcctgat ccgccagcac cagttccaca aggtcgagat ggtctccatc
     2221 gcccgccccg aggactccga cgccgagcac gagcggatga caaactgcgc cgagaccgtg
     2281 ctgcagaagc tggagctccc ctatcgccgg atgctgctgt gcaagggcga catgggcttc
     2341 acggcgaaga agacgttcga cctcgaggtt tggctgccgt cgcaggcgac ctatcgcgag
     2401 atcagctcct gctccaactg cggggatttt caggcgcgtc gcatggacgc ccggttcaag
     2461 cgcgcgggcg agaagaagac cgagttcgtg cacacgctga acggctcggg tctggccgtt
     2521 ggtcgcactc ttgtcgccat catggagaac tatcaggacg aggacggccg catcgctgtc
     2581 cctgccgccc tgcaacccta tatgggtggc ctgacccatg ttggagtgta agccatgacc
     2641 cgcatcgccc tcgcccccgc cctgatcgcc accgccctga tcgccgccgt cacagcaggc
     2701 gcggcctcgg cccagatcaa gccgccgatc gatccatccg cgacgacctt cgccggttgg
     2761 gtccgggtct cccacggcga gttccagctg tatgagaccc aacgccagat ctcagaaccc
     2821 ttctctcggc cctgcatctc cggcgccctg ccgcgcaacc tgcagcgggc gtcgggcgac
     2881 atcagcggac aacaggtgac cttcaccggc aaggccgtcg cctgggccga acgcggtacg
     2941 cgaaccacgc tcaatcatca gggctcacgc atcgtcaacg aatgcggcgg cgactacgtc
     3001 atcgccgccg aaagcgtccg ggtgctgcgc gccggatgag gatactcctg accaacgacg
     3061 acgggatcga ggccgaggga ctggcgtcgc tggaacggat cgcacgggcg ctttcggacc
     3121 cggcctcaag tagccctacg ggcggtaggc cccaagaaaa cgaagtctgg atcgttgcgc
     3181 ccgcgaccga acagtcgggc aagggcaggg gcatcaccct gaccgagccg ctgcgcgtca
     3241 acaagctggg cgacaagcgg ttctcggtca cgggcacgcc gaccgattgc gtcgttctgg
     3301 cgttcgatct gatggatgcg aagcccgatc tggtgctgtc cggcgtcaac cgcggccaca
     3361 acgttggcga ggactgctcc tattccggca cggtcgccgg ggcattgcag ggcatggcgc
     3421 tgggcgtccg ctcgatcgcc ctgtcgcagt cgctggaccg gtttcacgac gacgtgacgg
     3481 cgcattggga gacggcggag acctatgcgc cggcgatcat cgcgaccttg ctggcgcagg
     3541 actggcagcc gggcgtcgtc atgaacctga acttcccggc tcgccctcct gaacaggtga
     3601 aagaggtcga ggtgaccgtg cagggcttcc gccagatcgg cgagatgcat ccggtcaagc
     3661 gcaccgacct gcgcggtcgc gactattact ggatgagctt tcggggcgcg aaacaggatc
     3721 atgcgccggg caccgacctg cgggccatgg acgacggcaa gatctcggtc acgccgctcc
     3781 atattgacct gacgcataag gccagcgtcc acgacctgaa gggcgttctg ggcggggttc
     3841 ctccgaaagc catcgcagcc gaatgaacct caaggacgat cgcgcgggcc gattgatcct
     3901 gtccctgcgt cagcagggag tcacggatcc gcgcgtactc ggcgcgatgg agtcgatcga
     3961 ccgcgccgtg ttcgtgcacg agaaattcct cgatcaggcg tgggaggatc aggcgctgcc
     4021 gatcgattgc gcccagacga tttcacagcc ctatatcgtc ggtctgatga cccaggcgct
     4081 ggacgtccag ccgcgccacc gggtgctcga gatcggcacg ggcagcggct atcagtgcgc
     4141 cgtgctcagt cggctggcgc gctacgttta ttccgtcgag cgctatcgca gccttatggt
     4201 cgaggccgag ggcaagctga aactgctcgg catcgacaac gtcataaccc agcactcaga
     4261 cggcggtctc ggctggatcc agcaggcgcc gtttgaccgc atcatggtca cggcagcgtc
     4321 accgaccgaa ccgacggaac tgctgaagca gttgaagccg ggcggggtgc tggtttgccc
     4381 ggtcgggcgg acgtcggtgc agatgctgca tcgctacacc ggccagcctg atggcagctt
     4441 ccacagggaa agcctgcgcg aggtgcggtt cgtcccgctg gtcgagggga cggcgaagga
     4501 ggggtgacgg tggtgcggcg tcacctctcc gcgcgcgaag gcgcgggggg aggacgatcg
     4561 tcgcgcttgc ggcgatccgg agggggcgac acggtgtcaa ccggtgggcc aacatcttcg
     4621 ccggtgtcgc ccccacctgg tcgctgaggc gacctgtcct ccctattcgc cttcgcggat
     4681 agggaggtga gcgccgaggt tgcgccccgc aatcttcccc cgttaacctt cgttccccac
     4741 tttcgcccgg tttcgcgggc ttggcttatg gctaacggcg gggcacatct gggtcaggat
     4801 tcgggttctg acttcgggcc aagatttcag gagagcgcga tgaacggaat gacgagctgg
     4861 atcaaggctc tgatcgtcac gggcgcggcg ctgaccgtga ctgcttgcgc gagctatccg
     4921 accgagccgc gctattcgac gcgcgccgac cctgcagcgg tcccgaattc ac
//
LOCUS       DASVTM010000028         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_959424,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000028 DASVTM010000000
VERSION     DASVTM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959424"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VF633_00640"
     CDS             <1..1026
                     /locus_tag="VF633_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tipN"
                     /protein_id="HEX8469595.1"
                     /translation="ERLGEALFDAAQKADQAADSRIEGARRIVGETAVLVDQAGQQAV
                     EKLERTMDRMVRALGEVDASISELDERTARLPEEARARANEVRNTVEQGLAALSAASR
                     KAAEDTEALDVGFQERVRRNYDMLNEAVRLMGVVSGDSPSSRRREPTPSAEEPRSRPS
                     AADERSFGLRGRLRLEPTDESVRRSPTPPVETPGLDWADLVNDGASQQPVEPMDLDQP
                     VMPSDADAMSDRITAAIRRMGVDPNALLPRSRVEEAADAFANRDPDRARQIVRRVAPA
                     AVRSVSRRVMSDPELKSDAERYVRAYSQKLANAARSGDAPSVLAALATDAGRAFMLLD
                     AAVGDLN"
     gene            1162..1761
                     /locus_tag="VF633_00645"
     CDS             1162..1761
                     /locus_tag="VF633_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469596.1"
                     /translation="MLDLPAEKQPDRRPAIVICAYDVDDVAWDPVEDLSGQPWSPTGA
                     RTVAIPADAPAQLAETISLHLRDPDCRAVLLVGRTHRSGGFRVQMRAENRALTGGEKL
                     SHTGPAMARATAPVADMVRALTAAGLHADASSEGEDDVGSFLLYSILTGLRDDADVPA
                     VGLLRAPTDVSDEMVRKGVKTAAAAMARTLAPLPRSRAI"
     gene            complement(1758..>2645)
                     /locus_tag="VF633_00650"
     CDS             complement(1758..>2645)
                     /locus_tag="VF633_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8469597.1"
                     /translation="APLWFVAATAFGAIIPLSDVMTLRLARREGFSFSVPRGFGSAAF
                     VGANVLMGALLVKAPVDAVLIWCVVAGVAMAVCAHWVLPAELVDDAPAGETPATGWAR
                     FAGMGRLLKDRTFMTAIFAVGCIQATHAFYYGFSAISWKAQGIPESMTGLLWAASVVI
                     EIGFMWWGEPWRRARGIGPLTVLGIGAGAAMLRWTALAFAPPLWLLWPLQTLHALSFA
                     ATYLAGVQLVERLSPPGSQTVAQTLSSVLSAGVLIGLATLAAGPLYDAFGVKGYLAMT
                     AMAVVGGLAALRLKSALKA"
BASE COUNT          479 a          996 c          802 g          368 t
ORIGIN      
        1 gaacgtctcg gcgaagccct gttcgacgcc gcccaaaaag cggatcaagc cgctgacagc
       61 cgtatcgagg gcgcccgccg catcgtcggc gagactgccg tccttgtcga tcaggctggt
      121 caacaggccg tcgaaaaact cgagcgcacc atggatcgca tggttcgcgc cctcggcgag
      181 gtcgacgcct ccatcagcga gctcgacgag cgcacagccc gcctgcccga agaagcccgc
      241 gcccgcgcca acgaggtccg taacacggtg gaacagggcc ttgccgcgct ctccgccgcc
      301 tctcgcaagg ccgcagagga caccgaggcc cttgacgtag ggttccagga acgcgtccgt
      361 cgcaactacg acatgctaaa cgaagcggtc cggctgatgg gcgtggtctc cggtgacagt
      421 ccaagctcgc gccgtcgcga acccacaccg tcagcggagg agccgcgaag ccgaccgtca
      481 gcagccgatg agcgcagctt cggcctccgc ggtcgtctgc gtctcgaacc gaccgatgaa
      541 tccgttcgcc gttcgcccac gccccccgta gagacccccg gcctggactg ggccgatctg
      601 gtcaacgacg gcgccagcca acagccggtc gaacctatgg acctcgacca gccggtcatg
      661 ccgtcggacg ccgacgccat gtccgaccgg atcaccgccg cgatccgccg catgggcgtc
      721 gatcccaacg ccctgctgcc gcgctcgcgc gtggaagagg cggccgacgc attcgccaac
      781 cgcgatcccg accgcgcgcg ccagatcgtc cgccgggttg ccccggcggc cgttcgcagt
      841 gtctcgcgtc gtgtcatgag cgatccggag ttgaagtcgg acgcggaacg ttatgtccgg
      901 gcttactcgc agaagctggc gaacgccgct cgctctggcg atgcgccttc ggttctggcg
      961 gccctcgcaa ccgacgccgg acgcgccttc atgcttctgg acgccgcggt gggcgacctc
     1021 aactgaaggt cggcgggtca cgcgtctgtt cttgcggcac tgtgacggga aaccgacgca
     1081 ataggacttg acggcgcgac cgggatgccg acacctgaag tctgcctgtc acgtcattgt
     1141 cacgcacgcc aagcagggtt catgctcgac ctcccagccg agaaacagcc ggatcgcaga
     1201 ccggcgatcg tcatctgtgc ctacgatgtc gacgatgtcg cgtgggatcc cgtggaagac
     1261 ctgtcgggtc agccatggag tcccaccgga gcgcgcacgg tggcgattcc ggcggacgca
     1321 ccggcccaac tcgccgaaac catcagcctg cacctgcgcg accctgactg ccgcgctgtc
     1381 cttctggtcg gacgcaccca caggagcggg ggctttcgag ttcagatgcg ggccgaaaac
     1441 cgggccctga ccggcggtga aaagctgtcc cacaccggac ccgccatggc ccgcgccacg
     1501 gctccggtag ccgatatggt gcgcgccctg actgcagccg gcctgcacgc agacgccagt
     1561 tccgagggcg aagacgatgt cggcagtttc ctgctttaca gcatcctgac tggcctgcgg
     1621 gacgacgctg acgtcccggc tgtcggcctg ttgcgcgccc cgacggacgt ctccgacgag
     1681 atggtccgca aaggcgtcaa gaccgctgcc gccgccatgg cccgcaccct ggctcccctg
     1741 ccccgtagcc gcgcgatcta ggctttcaag gcggacttca gcctcaacgc cgcaagcccg
     1801 ccgacgaccg ccatcgccgt catggctaga tagcccttca ctccgaatgc gtcgtaaagt
     1861 ggtcccgcag ccagcgtcgc cagtccgatc aacacgcccg ccgacagcac cgagctcagc
     1921 gtctgggcca cggtctgtga ccccggcggg ctcagccgct ccaccaattg cactccggcc
     1981 agataggtcg cggcgaagct aagcgcatgc agggtctgca acggccacag caaccacagc
     2041 ggtggcgcga acgccaacgc cgtccagcgc agcatcgccg cgcccgctcc gatccccagc
     2101 accgtcagcg gaccgatccc tcgcgcgcgc cgccacggct cgccccacca catgaaaccg
     2161 atctcgatca cgaccgacgc cgcccacagc aggccagtca tgctttccgg gatgccctga
     2221 gccttccagg agatggccga aaacccgtag tagaacgcgt gggtcgcctg aatgcagccg
     2281 accgcaaaga tggcggtcat gaaggtccgg tccttcagca gccgccccat gcccgcgaac
     2341 cgcgcccacc cggtcgctgg cgtctcgcca gcaggcgcgt catcgaccag ttccgccggc
     2401 agcacccagt gcgcgcacac cgccatcgcc acgcccgcca ccacgcacca gatcagcacg
     2461 gcatcgaccg gcgccttcac cagcagagcc cccatcagca cattcgcccc cacgaaggcc
     2521 gccgagccga acccacgcgg gaccgagaac gagaaaccct cacgccgagc cagcctcaag
     2581 gtcatcacgt cgcttagcgg gatgatcgcc ccgaacgccg tcgccgccac gaaccacagc
     2641 ggggc
//
LOCUS       DASVTM010000029         7767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_694930,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000029 DASVTM010000000
VERSION     DASVTM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694930"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VF633_00655"
     CDS             <1..620
                     /locus_tag="VF633_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX8469598.1"
                     /translation="TVREAMIALEIRGLVEARPGSGIYVTGAAPTPGPAPELDIGAFE
                     LTEARRLFEGEACALAASAITDGELAELDSIVADMIGENTMVSSIGEQADRRFHMTIA
                     KATRNAAIVMTVENLWDLRWRSPLCAAMLERARQVGVRPLIEDHEVILNALKARDPRA
                     ARNAMRAHLSRVIEGLLTATEMDAMAQTRSDVEARRLEMARRTAI"
     gene            complement(637..1893)
                     /locus_tag="VF633_00660"
     CDS             complement(637..1893)
                     /locus_tag="VF633_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX8469599.1"
                     /translation="MKRPLSLLIAGALALCPIMASAESPVCDGSSGYADAFDGRRTFL
                     WRPDWLAMTKARLADDPSLQPARAAMLARANAALDHAPYTVVDKAQTPASGDKHDYMS
                     MGPYWWPDRSKPNGQPYLRRDGQINPERETNAFDLADLEAMSQDVQALSLAYYFTGDE
                     RYAAKASEFIKVWFLTPATRMNPNLNHGQAVPGVVSGRAEGVIDAVRLVRVVESLGLL
                     DPSSSLTDEDRAGLRDWFAALVEWMATSPIGRDEKAATNNHGVYYDMLISEFALYSGM
                     DDVARSVAGRFGPTRLALQVAPDGSLPQELARTRSLHYTAWTLTASFDIAQLGQCVGV
                     DVWNYATPDGRSLRKATDFLAGWAGRETEWRWPELDKTETLGLYEVLQRGAWAWGDRS
                     LAEKAAGYRERNADLDLNLRVPPYAP"
     gene            complement(1946..4858)
                     /locus_tag="VF633_00665"
     CDS             complement(1946..4858)
                     /locus_tag="VF633_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469600.1"
                     /translation="MTIHSKTLTRAMLLASVASVFAIPAMAQTTPAPTAEQTPQQTAP
                     EDGAAVDDIVVTGIRATQRSSIAVKRDSAVVVDAIVNDEIGSQPDNSVGDTLERITGV
                     TADRFKGNANELSVRGLGPTLSFSTFNGREVSTAGPDRSVAFQQFPSELVNGVLVYKT
                     QQADFTEGGIAGVIDLRSIRPLEAKPRIQAEYRGVYQEKEDAATGRDGLGYRANISVT
                     NRWMDTSIGDIGVSLGYQHQDQAAPEDYYTTNSAFIPCSTSAINPALITGTAAQQAAA
                     GASFNCALRPTLSAANQAGTTTTPLYFATTSRAFQQKTTSEIRDGLIGALQWRPNDRL
                     DFVLDGQYSNRSSLESRNIFAITEAARGIQPLIIGGQPGGEGFSDGALISYTGNSNLE
                     IQLEDRLREEEYFGGGLAARYSGDRLTVNTDISYSQSVRTELQKATNMRSGRRVVYTL
                     DASDDVVPNITFQNFDINDPANFLTLVPASNTNYARYRRVTDRKDSIAAVRSDAVYDL
                     GEGFFRNVAFGARYSEHRRETDLNNNVDVPIPLARDGLTSAQLVAQANANCRVPFGTS
                     NFMAESPTNVRSWAQFNNDCLFNTYTGRDTNPLLADSRGPEDTNVTEKIAALYLMTTF
                     GSEIRGIPFSGNAGLRYVRTTVDATGYRSAVTVTPSVAGSAATIVVVPGTLSEVSGSG
                     EYDYLLPSLNLNFDLNDTTRLRFGVSKSIARVGIEEFNVGISPIVDPTATTVAGVLAN
                     SQTGNPNLKPLESWNFDTSLEFYPNRDTILSFATYYKLIKGAAFDAVQPFTTSIIANG
                     TEVTFNAVAPTNDTEQRHLYGFEVTGNYVFSWLPGLWSGLGATAGYNYAEADFEFPDP
                     SAVAPYVEPANLRGLSKHNFNGSVFWEGGGLSLRASYRYRSDYSKPNSSTNRSVQGSG
                     YLNLSAQYNLTDNIQLKAQALNVTNERDIMYKPGEDAITEVSESSPQYYFGVRFRY"
     gene            5003..6199
                     /locus_tag="VF633_00670"
     CDS             5003..6199
                     /locus_tag="VF633_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX8469601.1"
                     /translation="MMTTKGWRRAVLVAAGLLAAGGCAAGPIAPAPAAFAAPPASAGA
                     RDVAVLAQARRVADWQLSHRADFSYVQTFRDQTADPIDWIQATFYAGLADFADATGDT
                     AYAQAVERHGEEAGWGFGSRPRHADADAIGHAWIWAAGRVQGVARERRLAPIRARFNA
                     VLATPSDVALTFDEGRGERPCQVRWCWSDAVFMAPPVWTALSQVTGDDRYRVHADREL
                     KATIAALYDRDEHLFYRDSRFIGRPGPSGRKVFWSRGNGWSYAGMAKVLQTIPADDPN
                     RAEYEVLFRQMSERLRALQGAEGYWAVSLLDPGGPPETSGTAFFVYGLAWGVNAGLLD
                     RAEYGPAVAKGWAALDRAVQPDGHLGWVQQVGYAPDRVDAADTQLYGVGAFLMAASEV
                     SRRADW"
     gene            6316..>7767
                     /locus_tag="VF633_00675"
     CDS             6316..>7767
                     /locus_tag="VF633_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469602.1"
                     /translation="MSRSTLLAIAAVLASAACAHAEPARLSTGAISDPRGAVYHQAPP
                     MNVTVATRITPQVEALLEQLQAQGRDYRLDGVEVFSANDKFLPGKIAIAMAHVLTATP
                     RTDPNFANYLNGFRRLADLTVDEPNDSWGIYYYASALHDLEVAGLLDEAVSPETLAKL
                     RVKLDWRRFVREDGLTLIDLPNNYFGVAFSIARLRYLLGWEDASASEALLAKTLDHFR
                     EYSGEYGFADETNGEGRFDRYSVLLIGEIAQRFIETGVAPPPEVKTWLRKSVDLMLPR
                     LNLRGEGFEYGRSIGTYGETAFLEVLTAAAALDVLTDQEKAMAYAFSSRVAARYADFW
                     LDPATGSVNLWDHGRDTDNYRGKHRILGENLSLGHQLFYTNAIWNRLGLEDATPDVGF
                     EAWLAALPRSTTTWFARGDHDRLLVTYRDGERVIGLPLINGGATQHMNTPYFAIPFSP
                     GVLAGSADETYPQLLPRLTLEDGSVLMPLAYFQN"
BASE COUNT         1313 a         2343 c         2697 g         1414 t
ORIGIN      
        1 cgacggtgcg cgaagcgatg atcgcgctcg aaatccgggg tctggttgag gctcgacccg
       61 gatccgggat ctacgtcacc ggagcggcgc cgacgccagg acccgcgccc gaactcgaca
      121 tcggagcctt cgagctgacc gaagcgcggc gactgttcga gggcgaggcc tgcgcgctcg
      181 ccgccagcgc catcaccgac ggggagctgg ccgagctgga cagtatcgtc gccgacatga
      241 tcggcgagaa cactatggtc tcctcgatcg gcgaacaggc cgatcgccgc ttccacatga
      301 ccatcgccaa ggccacgcga aacgccgcta tcgtgatgac cgtcgagaac ctgtgggacc
      361 tgcgctggcg ctcgccgttg tgcgcggcga tgctggaacg ggcgcggcag gtcggcgtgc
      421 ggcctttgat cgaggatcac gaggttatcc tgaacgcgct gaaggcgcgc gacccgcgcg
      481 cggcgcgaaa cgccatgcgc gcgcatctga gccgggtgat cgagggcctg cttacagcaa
      541 cggagatgga cgcgatggcc cagacccgtt cggacgtcga ggctcgtcgg ctggagatgg
      601 cgcgccggac cgcgatctag gctgacctgt ccggtctcag ggcgcatagg gcgggaccct
      661 gaggttcagg tcgagatcgg cgttacgctc gcgatagccg gcagcctttt cagcgagcga
      721 acgatcgccc caggcccagg ctccgcgttg caggacttca tacagtccca aggtctcggt
      781 cttgtcgagc tcgggccagc gccattcggt ctcgcgaccc gcccagcctg caaggaagtc
      841 cgtcgccttt cgcagcgagc ggccgtcggg cgtggcgtaa ttccagacat ccacgccgac
      901 gcactgtccg agctgggcga tgtcgaacga cgccgtcagc gtccacgccg tgtagtgcag
      961 cgaccgggtc cgggcgagtt cttgcggcag gcttccgtcc ggtgcgacct gcagcgcgag
     1021 acgcgtcggg ccgaagcggc cggcgacaga gcgggccacg tcgtccatgc cggagtacag
     1081 ggcgaactcg ctgatcagca tgtcgtaata gacgccgtgg ttgttggtcg ccgccttctc
     1141 gtcacggccg atcgggctgg tcgccatcca ttcgacaagg gcggcaaacc agtcgcgaag
     1201 gcccgcacgg tcttcgtcgg tcaggctgga agagggatcg agcaggccga gcgactcgac
     1261 cacacgcacc agccgcaccg catcgatgac cccttcggcc cggcccgaga cgacgcccgg
     1321 gaccgcctga ccgtggttta ggttcgggtt catccgcgtt gcgggcgtga ggaaccagac
     1381 cttgatgaac tccgaggcct tggcggcgta gcgttcgtcg ccggtgaagt aataggccag
     1441 cgacagggcc tgcacgtcct ggctcatcgc ctccagatcg gccaagtcga aggcgttggt
     1501 ctcgcgctcg ggattgatct gcccgtcgcg ccggagatag ggctggccgt tcggtttcga
     1561 ccgatcaggc caccagtacg ggcccatgct catatagtcg tgcttgtcgc cgctggcggg
     1621 cgtctgagcc ttgtcgacga cggtgtaggg cgcgtggtcc agcgcggcgt tcgcccgagc
     1681 gagcatcgcc gctcgggcgg gctgaaggct gggatcatcg gccaatctag ccttggtcat
     1741 ggcgagccag tctggccgcc aaaggaaggt tcggcgacca tcgaaggcgt cggcatagcc
     1801 gcttgagcca tcgcagacgg gactctcggc gctggccatg atggggcaca gggccaaggc
     1861 gccggcgatc aggagggaga ggggacgctt catgagtttc acaggaatgg gacgccccgg
     1921 cccgcgaagg ccggggccgt cgggatcagt aacggaagcg gacgccgaaa tagtactgtg
     1981 gcgagctctc gctgacctcg gtgatcgcgt cctcgccggg cttgtacatg atgtcccgct
     2041 cgttggtgac gttgagcgcc tgcgccttga gctggatgtt gtcggtgagg ttgtactggg
     2101 ccgacaggtt cagatagccg gagccctgga cgctgcggtt ggtgctcgag ttcggcttgg
     2161 aatagtccga gcggtagcga tacgacgcgc gcagcgacag accgccgccc tcccagaaga
     2221 ccgagccgtt gaagttatgc ttcgaaagac cgcgcaggtt ggccggctcg acatagggcg
     2281 ccacggctga cggatcagga aactcgaaat cggcttcggc gtagttgtag cctgcagtcg
     2341 cgccgaggcc cgaccagagg cccggcagcc agctgaacac atagttgccg gtgacttcga
     2401 agccatagag gtggcgctgc tcggtatcgt tggtcggggc gacagcgttg aaggtgactt
     2461 ccgtgccgtt ggcgatgatc gaggtggtga agggctgaac cgcgtcgaag gcggcgccct
     2521 tgatcagttt gtagtaggtg gcgaacgaca ggatggtgtc gcggttggga tagaactcca
     2581 gcgacgtatc gaaattccac gactccagcg gcttcaggtt cggattgccg gtctgggagt
     2641 tggccaacac gcctgcgaca gtcgtcgccg tcggatcgac gatcgggctg atgccgacgt
     2701 tgaactcctc gatgccgacc cgggcgatgg acttcgatac gccgaagcgc agccgggtgg
     2761 tgtcgttgag atcgaagttc aggttaaggc ttggcagcaa atagtcgtac tcgcccgagc
     2821 cggaaacttc gctcagcgtt ccgggcacca ccacgatagt cgccgccgaa ccggccaccg
     2881 aaggggtgac ggtgacggcc gaacggtacc cggttgcatc cacggtcgtg cgaacgtagc
     2941 gcaggccggc gttgccgctg aacgggatgc cacggatctc cgatccgaat gtggtcatca
     3001 gatagagggc ggcgatcttc tcggtgacgt tggtgtcttc aggaccgcgc gagtctgcca
     3061 gcagcggatt ggtgtcgcgt cccgtgtagg tattgaacag gcagtcgttg ttgaactgtg
     3121 cccacgaccg gacgttggtc ggcgactcgg ccatgaagtt gctggtgccg aaggggacgc
     3181 ggcagttggc gttggcctga gccaccaact gggctgaagt caggccgtcg cgagccagcg
     3241 ggatggggac atcgacgttg ttgttgagat cggtttcgcg gcggtgctcc gaatagcggg
     3301 cgccaaatgc gacgttcctg aagaagcctt ctccgaggtc gtagacggca tcagagcgga
     3361 cggccgcgat ggagtccttg cggtccgtga cccgccgata gcgggcgtag ttggtgttgg
     3421 acgcgggaac gagcgtcagg aagttggccg gatcgttgat gtcgaaattc tggaaggtga
     3481 tattcggcac gacatcgtcg gaggcgtcca gcgtataaac cacgcggcgg cccgaccgca
     3541 tgttggtggc cttctgcagc tcggtgcgga ccgactggga gtaggagatg tcggtgttga
     3601 ccgtcagtcg atcgccgctg tagcgcgccg ccagaccgcc accgaaatac tcttcttcgc
     3661 ggagccggtc ctcaagctgg atttcgagat tggagttgcc ggtgtagctg atcagggcgc
     3721 cgtcgctgaa cccttcgccg cccggctgac cgccgatgat caacggctgg atacctcggg
     3781 ccgcctcggt gatcgcgaag atattacggc tctcaaggct ggagcggttc gaatattggc
     3841 cgtccaggac gaaatcgagc cggtcattgg gccgccactg cagggccccg atcaggccgt
     3901 cccggatttc gctggtggtc ttctgctgga aggctcgcga tgtcgtggcg aaatacagcg
     3961 gagtggtcgt ggtgccggcc tggttggcgg cgctgagcgt cggccgcaag gcgcagttga
     4021 agctggcgcc cgctgctgcc tgttgcgccg ccgtgcccgt gatcagggcc gggttgatgg
     4081 cggaggtgga gcaggggata aaggcggagt tggtggtata gtagtcttcc ggcgcggcct
     4141 gatcctgatg ctggtagccg aggctcaccc cgatgtcgcc gatggaggtg tccatccagc
     4201 ggttggtgac cgagatattg gcgcggtagc cgagaccatc acggccggtc gcggcgtctt
     4261 ccttctcttg ataaacgccg cggtattcgg cctggatacg gggcttggcc tcaagcgggc
     4321 ggatcgagcg caggtcgatc acgccggcga tgccgccctc ggtgaagtct gcctgctggg
     4381 tcttgtaaac gagcacgccg ttgacgagtt cggaggggaa ctgctggaaa gcgaccgacc
     4441 ggtcgggccc cgccgtcgag acttcacggc cgttgaaggt cgagaaggaa agggtcggcc
     4501 cgagaccccg gacagaaagt tcgttggcgt tgcccttgaa ccggtcggcg gtgacgccgg
     4561 tgatccgctc gagcgtgtcg cccacggagt tatcgggttg agacccgatc tcgtcgttga
     4621 cgatggcgtc gaccacgacg gccgagtcgc gcttcaccgc gatcgaacta cgctgggtgg
     4681 cccggatgcc ggtgacgacg atgtcatcaa cggcggctcc gtcttcggga gcagtctgct
     4741 gcggcgtttg ctccgctgtc ggagccgggg tggtctgggc catggctgga atggcgaata
     4801 cggacgcgac ggacgcgagc agcatggcgc gcgtgagagt cttcgagtgg attgtcatgg
     4861 tgtttcccct gtgatgtttc caagcgcccg tgaaccaggc gtcgttatga gaacaggcta
     4921 gaccggaagt gacaccggtg acaagattgg cctgaccaaa aaatgaaccg gcaagccata
     4981 ggcttcggtc aggaggaatg agatgatgac gaccaaaggg tggagacgcg ccgtgctggt
     5041 agcggccggg ctgctggcgg cgggcggttg cgccgccggt cctattgcgc cggcgcccgc
     5101 tgccttcgct gccccccctg catcagcggg cgcgcgagac gtggccgttc tggctcaggc
     5161 ccgtcgggtc gctgactggc aactgtctca cagggcggac ttcagctacg tccagacttt
     5221 ccgcgatcag acggctgatc cgatcgactg gattcaggcg accttctacg ccgggctggc
     5281 ggatttcgcc gacgccacgg gcgacacagc ctacgcgcaa gctgtcgaga gacacggcga
     5341 agaagccggc tggggattcg gttcgcgtcc acgtcatgcc gatgcggacg ctatcggcca
     5401 cgcctggatc tgggcggccg gtcgtgtgca gggtgtggcg cgagagcggc gtctggcgcc
     5461 catccgggcg cgcttcaacg cggtgttggc gacgcccagc gatgtcgccc tgaccttcga
     5521 cgagggcagg ggtgaacggc cctgccaagt ccgctggtgc tggagcgacg ccgtcttcat
     5581 ggcccctccg gtgtggactg ccttgtcaca agtgacgggc gacgatcgct atcgcgtcca
     5641 tgcggatcgc gaactgaagg cgacgatcgc agctctgtat gatcgcgacg agcatctgtt
     5701 ctatcgtgac agccggttta tcgggcgtcc gggcccgagc ggccggaagg tgttctggag
     5761 ccggggcaac ggatggtcct acgcaggcat ggccaaggtc ctgcagacga tccctgccga
     5821 cgacccgaac agggccgaat atgaggtgct cttccgacag atgtccgaac ggctgagagc
     5881 gctacagggc gccgaagggt attgggccgt ctctctgctc gatccgggtg ggccgccgga
     5941 aaccagcgga accgccttct tcgtctatgg gctggcgtgg ggcgtgaacg cgggcctgct
     6001 tgatcgggcc gaatacggcc ctgcggttgc gaagggctgg gccgctttgg accgcgccgt
     6061 gcaacccgat ggccatctcg gctgggtcca gcaggttggt tacgcgccag accgcgtcga
     6121 cgccgccgat acgcagcttt acggagtcgg cgccttcctg atggcagctt cggaagtctc
     6181 acgtcgagcg gattggtaag accggttgac ggtgcggtct gaccaccata gcgttggcac
     6241 gttccttccc aggagcagat gacctgtagg gagcggcgct cgcgtcgctc cctcttttgt
     6301 atcgcggagt cccggatgag cagatccacc cttttggcga tcgcggccgt tttggcatcc
     6361 gcagcttgcg cccacgcaga gccggctcgg ttgagcacag gcgccatctc cgaccctcgc
     6421 ggcgccgtct atcatcaggc gccgccgatg aacgtgacgg ttgccacacg catcacgccg
     6481 caggtcgaag ccttgctgga gcagcttcag gctcaaggcc gcgactatcg tctggatggg
     6541 gtcgaggtct tttccgctaa cgacaaattc ctccccggca agatcgccat cgccatggcc
     6601 catgtgctga cagcgacgcc tcgcaccgac ccgaacttcg ccaactactt gaacggcttc
     6661 cggcgtctgg ctgatctgac ggtcgacgag cccaatgaca gctggggcat ctactactac
     6721 gcctcggcgc ttcacgacct cgaggtggcc ggccttctgg acgaggcggt cagccctgag
     6781 accctggcca agctccgcgt caaacttgac tggcgccggt tcgtccgaga ggacggcctg
     6841 accctgatcg atcttccgaa caactatttc ggggtcgcgt tcagcatcgc ccggctccga
     6901 tatctgctcg gctgggagga cgcctcggcg agcgaggccc tgctggccaa gacgctcgat
     6961 catttccgcg aatattcggg cgagtacggc ttcgccgacg agaccaatgg cgagggtcgg
     7021 ttcgatcgct attccgtgct gctgatcggt gagatcgccc agcgattcat cgagaccggc
     7081 gtcgcgccac cgcctgaggt gaagacctgg ctgcgcaagt ccgtcgacct gatgctgccc
     7141 cgcctcaacc tgcggggcga gggcttcgag tacggccgca gcatcggcac ctacggcgag
     7201 accgcgtttc tggaagtgct gaccgccgcc gctgcgttgg acgtgctgac ggatcaggag
     7261 aaggcgatgg cctatgcgtt ctcgtcacgg gtcgcggcgc gctacgccga cttctggctg
     7321 gatcccgcga cgggttcggt gaacctctgg gaccacgggc gcgataccga caactatcgg
     7381 ggcaagcacc ggatcctggg cgagaacctc agtcttgggc accagctttt ctacaccaat
     7441 gcgatctgga accggctggg tctggaagac gcgacgccgg acgtcgggtt tgaggcctgg
     7501 ctggcggcgt tgccgcgatc gacgacgacc tggttcgcgc ggggcgatca tgacaggttg
     7561 ctggtgacct atcgcgacgg ggagcgggtg atcggcctgc cgctgatcaa cggcggcgcc
     7621 acccagcata tgaacacgcc gtatttcgcg atcccgtttt cgcccggtgt gttggcgggc
     7681 tcggcggacg agacctatcc gcaacttctg ccgcgcctga cgctcgagga cggatcggtg
     7741 ctgatgccgc tcgcgtattt ccagaac
//
LOCUS       DASVTM010000030         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_959573,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000030 DASVTM010000000
VERSION     DASVTM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959573"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VF633_00680"
     CDS             complement(<1..1199)
                     /locus_tag="VF633_00680"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX8469603.1"
                     /translation="MSEEFYRMRRLPPYVIAEVNAMRHAARTAGEDVIDLGMGNPDLP
                     PPPHVIDKLIEVARNPDAHGYSQSKGIPGLRRAQANYYARRFGVDLDPETEVIVTMGS
                     KEGLASLATAITEPGDVVLAPNPSYPIHTFGFIIAGAAIRSVPTTPDEKYFESLERAM
                     AFTVPRPKILVMNYPSNPTAETVDLAFYERVVAFAKANELWIISDLAYSELYYDGKPT
                     VSILQVPGARDVAIEFTSLSKTYSMAGWRIGFAVGNKRLIAAMARVKSYLDYGAFTPI
                     QAAAVAALNGPQDIVEKNRALYHKRRDVLVKSFGAAGWTIPAPAASMFAWAPLPPSMA
                     HLGSLEFSKQLLTHAKVAVAAGVGYGENGEGFVRIAMVENEQRIRQAARNIKTFLKSR
                     GVNGPFG"
     gene            complement(1292..1600)
                     /locus_tag="VF633_00685"
     CDS             complement(1292..1600)
                     /locus_tag="VF633_00685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8469604.1"
                     /translation="MTEPEIRHNEASHRYELTIDGQTAVVLYNEVGGGYLITETLVPV
                     PLEGKGVASRLAKHVLADIRERGLLVLPTCPFFSGYIQKHPADYADIVHPSYRIALGL
                     "
BASE COUNT          268 a          444 c          620 g          279 t
ORIGIN      
        1 tccccgaacg ggccgttgac gccgcgggac ttgaggaagg tcttgatgtt gcgggcggcc
       61 tgacggatgc gctgctcatt ctcgaccatg gcgatgcgga cgaagccctc gccgttctcg
      121 ccgtagccga cgccggctgc gaccgcgacc ttggcgtggg tcaggagctg tttggagaac
      181 tccagactgc cgagatgggc catcgagggg ggcaggggcg cccaggcgaa catcgaggcg
      241 gcgggggcgg ggatggtcca gccggcggcg ccgaagctct tgaccagcac gtcgcggcgc
      301 ttgtgataca gggcgcggtt cttttcgacg atgtcctgcg ggccgttcag ggcggcgacg
      361 gcggcggcct gaatgggggt gaaggcgccg tagtcgagat aggatttgac ccgcgccatg
      421 gccgcaatca gccgcttgtt gccgaccgcg aagccgatgc gccaaccggc catgctgtag
      481 gtcttgctga gggaggtgaa ctcgatggcg acatcgcggg cgcccggcac ctgcaggatc
      541 gagacggtcg gcttgccgtc gtaatagagc tctgaatagg cgaggtccga gatgatccag
      601 agctcgttgg ccttggcgaa ggcgaccacg cgctcataga aggcgaggtc caccgtctcg
      661 gccgtcgggt tggacggata gttcatcacc aggatcttgg gccggggcac ggtgaaggcc
      721 atggcccgct caagggactc gaaatacttc tcgtccggcg tggtcgggac gctgcggatg
      781 gcggcgccgg cgatgatgaa gccgaaggtg tggatcggat aggacgggtt gggggcgagg
      841 accacgtcgc cgggctcggt gatggcggtg gcgaggctgg ccagcccctc cttggagccc
      901 atggtgacga tgacctcggt ctcgggatcg aggtcgacgc cgaaccgacg ggcgtaatag
      961 ttggcttggg cgcggcgcag gccgggaatg cccttggact gggaatagcc gtgggcgtcc
     1021 ggattgcgcg cgacctcgat cagcttgtcg atgacgtgcg gaggcggcgg caggtccgga
     1081 ttgcccatgc cgagatcgat gacgtcctcg ccggcggtgc gcgcggcatg gcgcatcgca
     1141 ttgacctcgg cgatcacata gggcggcaga cgccgcatgc ggtagaattc ttcggacatc
     1201 acaggctcat cggaaaaggc gtcccgcgag gtgaagccag agcgcgccag acgcaagagg
     1261 cttgcgcgat ccagcggaag aaagtgctgc gttagagccc cagcgcaatc cggtagctgg
     1321 ggtggacgat gtcggcgtag tccgccgggt gcttctggat gtagccggag aagaaggggc
     1381 aggtcggcag gaccagcagg ccgcgttcgc ggatgtcggc gaggacgtgt ttggcgaggc
     1441 ggctggcgac gcctttgcct tcgagcggga ccgggacgag ggtttcggtg atcaaatagc
     1501 cgccgccgac ctcgttgtag agcaccacgg cggtctgacc gtcgatggtt agttcgtagc
     1561 ggtgggaggc ttcgttgtgg cggatttcgg gttcggtcat ggcggatgtc t
//
LOCUS       DASVTM010000031         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_3547, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000031 DASVTM010000000
VERSION     DASVTM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3547"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF633_00690"
     CDS             complement(<1..450)
                     /locus_tag="VF633_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469605.1"
                     /translation="MRRFLIWSGLAVVIGLILAVSGYLAYDYFYARFQPVTIARNQGQ
                     IQRLLDEASWVSAGGGDEPLYIIGYRDSPATQAYQRQEADKLKAGGVDVRIIMFARPD
                     KEGASQSTAAERATLAELWLTRDWSLYERWTATPSKDWTAAGIPMSDG"
     gene            622..1623
                     /locus_tag="VF633_00695"
     CDS             622..1623
                     /locus_tag="VF633_00695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8469606.1"
                     /translation="MTKLSILDLAPVPQGSDVSQALANTIDLARNAERLGYNRYWLAE
                     HHNMEGIASAATCVVIGAVAAATNTIRVGAGGVMLPNHAPLVIAEQFGTLNALYPGRI
                     DLGLGRAPGSDQATARALRRTLQGEVDAFPQDVMELLHWFKPADDNQAIRANPGEGQA
                     VPVWILGSSTYGAQLAAHLGLPYAFASHFAPGDMMSAIRVYRETFRPSEYLSEPYVML
                     GFNVFAANTDEEANLLFSSMQQAFVNLRSGRPGKLPPPVQYYEASLDSGAKGMIAQAL
                     SCSAVGSPATVKRGLESFIARTGADELMITSQIWSHEARVRSLEILADVGMGLAKAA"
     gene            complement(1627..>2730)
                     /locus_tag="VF633_00700"
     CDS             complement(1627..>2730)
                     /locus_tag="VF633_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8469607.1"
                     /translation="LVAVDPAWFDKSVKQLRGRWSGTRSQPVTEGETRLLAALHDDRP
                     GAESVLSPPPDPEQPDRNAGRRLRVWKQFFRSRRMAELPVSQHYFNVGHTSLNTPDIL
                     SSLKDHGVDTVVLLHDLIPITHPEFCRPGDGDKHRKRVLTALNYASRILVNSDYTAHE
                     LVLFAQREGLRPPPIEAIHLGLEPSFVARPTASTLRPYFVHVGTIEARKNLAFLLTIW
                     RRLEERMGDKAPRLVLVGRYGWENEAVLDHLERSPNLRGLVHQASNLADETLAILMSN
                     AQAVLAPSSVEGFDLPAVEACALGAPLIASDIPAHRELVPQAELIDPLDGLGWLAALE
                     RATLKKPEAPAYRAPTWDEHFDKVARTLGLTAA"
BASE COUNT          482 a          903 c          870 g          475 t
ORIGIN      
        1 gccgtcggac atcgggatgc cagccgccgt ccagtctttc gatggcgtcg ctgtccagcg
       61 ttcatacagg ctccagtccc gtgtcagcca cagctccgcg agcgtggcgc gctcggccgc
      121 cgtcgattgg gacgcgcctt ccttgtcggg gcgcgcgaac atgatgatcc ggacatcgac
      181 gccgccggcc ttcagcttgt ccgcttcctg acgctgatag gcctgggtgg ccgggctgtc
      241 gcgatatccg atgatgtaga ggggctcgtc cccaccgccg gctgacaccc acgacgcctc
      301 atcgagcaga cgctggatct gaccctggtt cctcgcgatc gtcacgggtt ggaagcgggc
      361 gtagaaatag tcgtacgcga ggtatccgct cacggcgagg ataaggccga tcacgaccgc
      421 caggcccgac cagatcagaa agcgacgcat acggcaaagg gctccggctt gggcattcgt
      481 ccaagtctaa tacctatcac cgatttgtgt cgcatccgaa cacccaccgg gcgaacccac
      541 acagtcaggg tccgcatggt ttgaaagcaa cagtccgagt tgcagggttt tcatcgcggc
      601 gaacctcccc atatcctgct catgaccaag ctctcgatcc tcgacctcgc tccggtgccg
      661 cagggctcgg atgtgtcaca ggcgctcgcc aacaccatcg accttgcgcg caacgccgag
      721 cgtctgggct acaaccgcta ctggctggca gagcaccaca acatggaagg catcgcctcg
      781 gcggcgactt gcgtcgtgat tggggcggtg gcagcggcga cgaacacgat ccgggtcggc
      841 gccggcgggg tcatgctgcc caaccacgcg cccttggtca tcgccgagca gttcggcacc
      901 ctgaatgccc tctatcccgg gcgaatcgac ctcggtctcg ggcgcgctcc gggctccgat
      961 caagctaccg cacgggcgct gcgccgcacg ctgcagggcg aggtcgacgc ctttccgcaa
     1021 gacgtcatgg agcttctgca ctggttcaag ccggccgacg acaatcaggc catccgcgcc
     1081 aaccccggcg agggtcaggc cgtgccggtc tggatcctcg gctcctcgac ctacggcgcg
     1141 cagctggccg ctcatctcgg cctgccctac gccttcgcca gccatttcgc gcccggcgac
     1201 atgatgtccg ccattcgagt ctaccgcgag accttccgcc cgtcggaata tctgtcggag
     1261 ccctatgtga tgctcggctt caacgtcttc gcggccaaca ccgacgagga ggcgaacctg
     1321 ttgttcagtt cgatgcagca ggccttcgtc aatctgcgct ccggccgtcc cggcaagctt
     1381 ccgcctccag tgcagtacta tgaggcttcg ctagattccg gcgccaaggg catgatcgcc
     1441 caggcgctct cctgttcggc tgtaggatct ccggcgacag tcaaacgcgg actggagagc
     1501 ttcatcgccc gcaccggagc ggacgagctg atgatcacca gccagatctg gtcccacgaa
     1561 gcccgggtgc gctcattgga aatcctcgcc gacgtcggga tgggcctggc caaggcggcc
     1621 tgatgttcag gcggccgtaa ggcccagcgt ccgcgccacc ttgtcgaaat gctcgtccca
     1681 cgtgggggcg cggtaggcgg gggcctcggg ctttttcaac gtcgctcgct cgagcgccgc
     1741 cagccaaccg agaccgtcca gcggatcgat caactcggcc tgcgggacca gttcgcggtg
     1801 ggcagggatg tccgaagcga tcagcggcgc ccccagagcg caggcctcga ccgccggaag
     1861 atcgaacccc tccaccgacg aaggtgccaa caccgcctgg gcattagaca tcaggatggc
     1921 gagagtttcg tcggccaggt tcgacgcctg atgcacgaga ccgcgcaggt ttggtgagcg
     1981 ctcgagatgg tcgagcaccg cctcgttttc ccagccatag cgaccgacca gcaccagacg
     2041 cggagccttg tcacccatcc gttcctcgag cctgcgccag atggtcagca ggaaggcgag
     2101 gttcttgcgc gcctcgatgg tgccgacatg aacgaagtag ggacgcaggg tcgaagctgt
     2161 cggtcgggcg acgaaactgg gttccagccc cagatggatg gcctcgatcg gcggcgggcg
     2221 caggccttcc cgttgggcaa acaggacaag ctcgtgcgcg gtgtaatccg agttcaccag
     2281 aatgcgcgac gcatagttca gcgcggtcag cacgcgtttg cgatgcttgt caccgtcccc
     2341 cgggcgacag aactcgggat gggtgatcgg gatcaggtca tgcagcagaa cgaccgtatc
     2401 gaccccatga tccttcaacg aagacaggat gtcgggcgta ttcaggctgg tgtggccgac
     2461 gttgaaataa tgctggctga cgggcagctc ggccatgcga cgcgagcgga agaactgctt
     2521 ccagacgcgc agccgacggc cggcgttgcg gtccggctgt tcgggatcgg gcggggggct
     2581 gaggacggac tccgctcccg gtcgatcgtc atgcagggcc gcaaggagtc gcgtctcgcc
     2641 ctcagtgaca ggctgggacc gggttccgct ccagcggccg cgcagttgtt tgacgctttt
     2701 gtcgaaccac gcgggatcga ccgctaccaa
//
LOCUS       DASVTM010000032         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_322048,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000032 DASVTM010000000
VERSION     DASVTM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322048"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="VF633_00705"
     CDS             <1..1406
                     /locus_tag="VF633_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469608.1"
                     /translation="DWAVRFGAVSQVLHIDDSQYGFKSLPPYERALSLAEPSNNDFNG
                     LSLSASGDYDWGVVKLSSAFQAHDLDRRYDATTVAARFGGTGGSLAYDETDDILAFAL
                     EASVTSPDTQSFRWLAGLSVSRYSHDRTSEMVDVAPDIRLYETTKQDQTNEAAVFGEI
                     AWALNDRLEITLGGRYFAAVMDSTIEAHRRGVLSDTFEGEIRDDDFSTKAVIEYAFRE
                     ELLVYAQTSLGYRMSGFNGGSVLDGVYGEPGTGAQPYRAFLPDNLFSHEIGLRWRTLD
                     DRLALRLAAFVVDWRSIQSDRVSRDGLPFTANIGSADNTGLEIEGVWRDDDWRIDFNL
                     MANDPRLNAEDDGYPLATDSDLPGVPKLMGAMSARRDGVLFDLPGWISGTIGYVGPSK
                     QRLTPTSLADMGDYFTSELAANISLGAWSATLRLDNLLGGAGNTFGYGNPFLVDQETV
                     TTPQRPRNLSLSFSRSF"
     gene            complement(1611..>1903)
                     /locus_tag="VF633_00710"
     CDS             complement(1611..>1903)
                     /locus_tag="VF633_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX8469609.1"
                     /translation="EYLGHPAEGEYPRASPLLANDETLRRQPPTLVITAEMDPLRDEG
                     QAYADRLRALGVAVEYVCTPGTIHAFVLFAGVLKRGDAGLTRIANAFRQMTR"
BASE COUNT          353 a          632 c          575 g          343 t
ORIGIN      
        1 acgactgggc ggtgcggttt ggcgcggtgt cccaggttct gcacatcgac gacagccagt
       61 atggcttcaa gtcgctgccg ccctacgagc gagccttgtc attagctgag cccagtaaca
      121 acgatttcaa cggcctctcc ctgtcagcga gcggtgatta cgactggggc gtagtgaagc
      181 tgagcagcgc ctttcaggct catgaccttg atcgtcgata cgatgcgacc accgtcgcag
      241 cccgctttgg cggaactggt ggttcgttag cctatgacga gaccgacgac attctcgcct
      301 tcgcgctgga ggcctccgtc acatccccgg acacgcagtc gttccgctgg ctcgccgggc
      361 tttctgtgtc tcgctacagc catgaccgga ccagcgagat ggtcgatgtc gcccctgata
      421 ttcgcctcta cgaaacgacc aaacaggacc agaccaatga ggcggcggtg ttcggcgaga
      481 tcgcctgggc gctcaacgac cggctcgaga tcacgctggg cggtcgctat ttcgccgccg
      541 tgatggactc gaccatcgaa gcgcatcgac gcggcgtgct gtccgacacg ttcgaaggcg
      601 agatcaggga cgacgatttc tcgaccaagg cggtgatcga atacgccttc cgcgaagagc
      661 tcctcgtcta tgcccagacc agcctcggct accgcatgag cggcttcaac ggaggctccg
      721 tgctggacgg cgtttacggc gagcccggaa ccggcgccca gccctatcgc gccttcctgc
      781 ccgacaacct gttcagccat gagatcggcc tgcgctggcg cacgctcgac gatcgtctgg
      841 ccctgcgtct ggccgccttc gtggtcgact ggcgttcgat ccagtcggac cgcgtatcgc
      901 gcgacggcct gcccttcacc gccaacatcg gttcagccga caacacgggg ctggaaatcg
      961 agggcgtatg gcgcgacgac gactggcgca tcgacttcaa cctgatggcc aacgaccccc
     1021 gcctgaacgc ggaagacgac ggctatcccc tagcgaccga cagcgatctg ccgggagtac
     1081 cgaagctgat gggcgcaatg agcgcgcgcc gcgacggcgt cttattcgac ctgccgggct
     1141 ggataagcgg aaccatcggc tacgtcggcc catcgaaaca acggctgacg cccacaagcc
     1201 tcgccgacat gggtgattat ttcacctccg aactggccgc taacatcagc ttgggcgcct
     1261 ggtccgcgac cctgcgcctt gacaatctgc tgggcggcgc aggcaacacc ttcggctacg
     1321 gcaatccatt cctcgtcgat caggaaacag tcaccacgcc ccagcgcccg cgaaatctgt
     1381 cgctttcctt cagcagaagt ttctagcggt ccgccgacat cagacattcc gagccgccgc
     1441 gttgtgtcag tggcggctaa ggtctcggaa gcgccataag ccgacgtcgc ccggcctgcc
     1501 cggacaaggc gtcggcagga cggcttggtt agcgccttta tcctcgtgca aagtgctgct
     1561 gcggaacggc ccaagcccct gaacgtcgtt gtgcgaccca acgcaagcga tcagcgcgtc
     1621 atttgccgga aggcgttagc gatccgcgtc aacccggcgt cgccgcgttt cagaactccg
     1681 gcaaacaaca caaaggcatg gattgtgccg ggcgtgcaga catattccac ggcaaccccc
     1741 aaggcccgca ggcgatcggc atacgcttgc ccctcgtctc gcagcggatc catctcggca
     1801 gtgatcacca gcgtcggcgg ctggcggcgc agggtttcgt cattggccaa caggggcgag
     1861 gcgcgggggt attcgccctc cgccggatgg ccgagatatt cgg
//
LOCUS       DASVTM010000033         9026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_587787,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000033 DASVTM010000000
VERSION     DASVTM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587787"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /gene="grpE"
                     /locus_tag="VF633_00715"
     CDS             <1..344
                     /gene="grpE"
                     /locus_tag="VF633_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX8469610.1"
                     /translation="KDAEGAAAGLVIGLELTEKSLLGAFASNGLKRVAPEAGEAFDPH
                     LHQAMMEQPSETVPGGAVIQTMQAGYELFGRTVRPAMVVVAAKGSGAKAASSAYAEAP
                     ATNGGSYDNKA"
     gene            complement(445..978)
                     /locus_tag="VF633_00720"
     CDS             complement(445..978)
                     /locus_tag="VF633_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8469611.1"
                     /translation="MSIDTVRDLLPAYAKDISLNLGSLASETVLTDQQKWGTFLASAH
                     AIGVVPVVKAIEAAAAEHLSPEAMNAAKAAAAIMGMNNIYYRSLHLMHNHEYQTLPAR
                     LRMNVLANPGVDKIDFELWSTAVSAINGCGACLDAHEGELKKHAVPSVQIQTALRIAA
                     VVHAASRIAASEAALAA"
     gene            complement(1162..1716)
                     /locus_tag="VF633_00725"
     CDS             complement(1162..1716)
                     /locus_tag="VF633_00725"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8469612.1"
                     /translation="MLGVGETLPNFKITGVKPGFNSHEENGVSAFEPLTETSFEGKWK
                     VIFFYPKDFTFVCPTEIAAFAKLGKDFEDRDTIVLGGSTDNEFVKLAWRRDHADLDKL
                     PIWQFADTGGKFARALGILDEAEGVALRATFVVDPHNVVQHVYVTNLNVGRAPDDTLR
                     VVDALQTDELCACNRPAGGEVIAA"
     gene            1982..4216
                     /gene="katG"
                     /locus_tag="VF633_00730"
     CDS             1982..4216
                     /gene="katG"
                     /locus_tag="VF633_00730"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX8469613.1"
                     /translation="MDGEQDPTPLNDPKKEPAALRTLLGRNNRDWWPNHLSMDILHQQ
                     GRSGDPMGDGFNYGEAFKKLDYAAVKRDLTALMTDSQPWWPADYGHYGPFFIRMAWHS
                     AGTYRTGDGRGGASAGQQRFAPLNSWPDNGNLDKARRLLWPIKQKYGANLSWADLMIL
                     AGNVAIESMGGPVFGFGGGRADVWEPEKDVYWGTEEEWLGETRIDEESGKALEAPLAA
                     IQMGLIYVNPEGPGGNAQALQSARDIRETFSRMGMNDEETVALTAGGHTFGKAHGAGD
                     ASKVGSDPEGADIAQQGLGWQSGHESGMGDHTITSGLEGAWTPTPTTWDMTFFDMLLD
                     HEYELVRSPAGAKQWQPVGNPEDTLAPGAHSPHRKVPTMMTTADMALKVDPAYRAICE
                     KFRSDPAYFGDAFARAWFKLTHRDMGPKSRYLGPEVPAEELIWQDPIPRRTGPAIGDA
                     DIRVLKDAIAASGLSVAELVKTAWASAATYRGSDHRGGANGGRLRLEPQRSWEVNEPD
                     QLARVLQVYEGIKAGSGTNVSIADLIVLGGSVGIEQAAQAAGHAIEVPFTPGRTDASA
                     EQTDVEGFAVLEPKADGFRNYLSVRFNVPTEELLVDRAQLLGLTAPEMAVLVGGLRVL
                     GANHGGTPHGVLTKRPGQLTNDFFVNLLDMTTAWKEVDDRGDEVFLGTDRITGEELWT
                     ATRTDLVFGSNSQLRALSEVYASADAGEKFVRDFANSWAKVMNADRFDVTLEEEFGTA
                     AVDA"
     gene            4319..5218
                     /locus_tag="VF633_00735"
     CDS             4319..5218
                     /locus_tag="VF633_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8469614.1"
                     /translation="MLPTLRQLQYLKLLAEHASFSRAAEAAHVSQPALSAGVQELEKI
                     LGAPVVERTRGNVQLTAVGIEAVKRAEDVLARTEDLVEAARSAGRPLSGRFRLGIIPT
                     VAPFLLPATLPALKTAYPALRLFIREDLTPRLIAGLKAGQLDAAVIALPYDAPGIEHA
                     HISDDEIVAAAPAGHALAAPGAIQSGSLKAEDLILLEDGHCLRDQALAAFDIEAPKGD
                     DVFAATSLHTLVQMVSSGLGVSFLPQMAVKAGLADTPSVVIRHITPRAGALAPSREIV
                     VAWRAGSSRAAEARLLAEAMSQD"
     gene            5293..5793
                     /locus_tag="VF633_00740"
     CDS             5293..5793
                     /locus_tag="VF633_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469615.1"
                     /translation="MRLSRSIAVAALIGGAAALSGCVSMSRSKSVSALDPALASGARV
                     SEIRLTTDDKVTVRPEFAGIFRDHVQKKLDGCATGAQPLRLEATISRFDRANPAQVML
                     IGGANVLRGHARLVDPATGRVVAEYEIGRTIMGSRFGIFQMAESEEQLSDAFGAELCD
                     QAFKAD"
     gene            complement(5799..6092)
                     /locus_tag="VF633_00745"
     CDS             complement(5799..6092)
                     /locus_tag="VF633_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469616.1"
                     /translation="MNRSPHILNVSSNLLGITLLIIAGLHLTDKAAQSLADEIAWLGA
                     VCFLLACFLSYLSIGNGRASARLEIWADRIFLTGMTSLFCAIVVLALPGSIMR"
     gene            complement(6136..6762)
                     /locus_tag="VF633_00750"
     CDS             complement(6136..6762)
                     /locus_tag="VF633_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8469617.1"
                     /translation="MSAHPDLLAIWARGWALTRGVSPPSWDDGGWRVEVGLDDQLRRY
                     VFAKADDAVARRAAAISEPCIFLKVCADAQEVRAILPNTWSLRPSGFLMTLDGAMSEG
                     PPPPQGYTTSLWQDGAVTFCTIRHASGAEAARGRFVIVDDRVIYDRIAVDPDHRRLGL
                     GRQVMRTLETAAGGPRKAVLVATEEGQALYRTLGWGLHSPYTTAVIPG"
     gene            6978..7613
                     /locus_tag="VF633_00755"
     CDS             6978..7613
                     /locus_tag="VF633_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX8469618.1"
                     /translation="MTVSRRAFAFGTAAIAGLAALPAAAQTSLSSEDRATLQTAQTYL
                     QNLSSAQGTFVETGPGGQQRRGRFYLQRPGKMRFEYTDPAGLLVVSDGYNVKRYDPRL
                     EVFRQVPLGATPLSTFLARNVRLDQGVRIDRVTRMQSGAFALTVRDSAKPNEGSVILA
                     FAGSPLRLQEWTITDAQGGRTRTQLTTLQPASGLAASLFQLRDPTRRQGRN"
     gene            7854..8651
                     /locus_tag="VF633_00760"
     CDS             7854..8651
                     /locus_tag="VF633_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8469619.1"
                     /translation="MTLRIATWNINSVRLRIEQVARFVTESETDVLCLQEIKCLEDQF
                     PRNAFAEMGMPYLRIAGQKGWHGVAIASRRPIIDAPALDVCRNKHARCVSALVEGVEI
                     QNFYIPAGGDLPDRELNEKFDHKMDYYARLTAEMAARDQLAPLLLAGDFNIAPSEFDV
                     WNHRYMSRVVSHTPGEVETLNRLQAAGGFSDVVRDAYPEPQKLASWWSYRAQDFRKSN
                     RGLRLDHLWTSPGLTPKVVPGSATIHDTVREWTQPSDHCPITVDLDV"
     gene            complement(8655..>9026)
                     /locus_tag="VF633_00765"
     CDS             complement(8655..>9026)
                     /locus_tag="VF633_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8469620.1"
                     /translation="DGGAALSVRRKLLRTRRDHLLARLAERFPDWVCPRPDGGLSIWA
                     RLPGPISSALTVQAAIEGLRLAAGPRFGVDGAFENRLRLPYTLPEAELDLAVERLAKA
                     YGKLGRQKFVTRSPLNADPVY"
BASE COUNT         1595 a         2923 c         2947 g         1561 t
ORIGIN      
        1 ccaaagacgc cgagggcgca gccgccggtc tcgtcatcgg tctggaactg accgagaaat
       61 ccctgctggg cgcgttcgcc tccaacggtc tcaagcgcgt cgctcctgaa gcgggcgagg
      121 ccttcgatcc gcatctgcat caggcgatga tggaacagcc gtcggaaacc gttccaggcg
      181 gcgccgtgat ccagaccatg caggccggtt acgaactgtt cggccgcacc gtgcgcccgg
      241 ccatggtcgt cgtcgccgcc aagggctcag gcgccaaagc cgcctccagc gcctacgccg
      301 aagcccccgc caccaacggt ggatcgtacg acaacaaggc gtaaaacgag aaaagggact
      361 gacgacgttg ccgctgtcag tccctcgttt cactctaaac gctcaagcag cgaggctccg
      421 cgagcctcgc gttagcgctc ttgcttaagc ggcgagcgcc gcttcagacg ccgcgatgcg
      481 gctggcggcg tggaccacag cggcgatacg cagcgccgtc tggatttgca ccgacgggac
      541 ggcgtgcttc ttcagttcgc cttcgtgagc gtcaaggcag gcgccgcaac cgttgatcgc
      601 cgagacggcg gtcgaccaaa gttcgaagtc gatcttgtcc acgcccggat tggccaggac
      661 gttcatccgc agacgggccg gcagggtctg gtactcgtgg ttgtgcatca ggtgcagcga
      721 ccgatagtag atgttgttca tgcccatgat ggcggcggcg gccttggcgg cgttcatcgc
      781 ttccggcgac aggtgttcgg cggcggcggc ctcgatcgcc ttgaccacgg gcacgacgcc
      841 gatggcatgg gccgaggcga ggaacgtgcc ccacttctgc tggtcggtca gcacggtctc
      901 ggaggccagc gaaccgaggt tcagggaaat gtccttggcg taggcgggaa gcagatcgcg
      961 gacggtgtcg atcgacatga tgatttttcc ttcggaaagc ggctaccgct cggctcgcgg
     1021 agcctcgctg cttgagccgc agatatgggg atgaccgtga gcgccgccat ttggccggac
     1081 gctggcgctg atggatggca ggtaaagaaa aggcggcggc ggtgatccgc cgccgccctt
     1141 ctgtttcgtc aggtcagacg cttaggctgc gatgacttcg ccgccggcag ggcggttgca
     1201 ggcgcacagt tcgtcggtct gcagggcgtc gacgacacgc agggtgtcgt caggcgcgcg
     1261 gccgacgttc aggttggtga cgtagacgtg ctggacgacg ttgtgcgggt cgacgacgaa
     1321 ggtcgcgcgc agggccacgc cttcagcttc gtcgaggatg ccaagggcgc gggcgaactt
     1381 gccgcccgtg tcggcgaact gccagatcgg cagtttgtcg aggtcggcgt ggtcgcggcg
     1441 ccaggccagc ttgacgaatt cgttgtcggt cgagccgccg agcacgatcg tgtcgcggtc
     1501 ttcgaagtcc ttgccgagct tggcgaaggc ggcgatttcg gtcgggcaga cgaaggtgaa
     1561 gtccttcgga tagaagaaga tgaccttcca cttgccttcg aatgaggttt cggtcagcgg
     1621 ctcgaacgcc gagacgccgt tttcttcgtg cgagttgaag ccgggcttta cgccggtgat
     1681 cttgaagttc ggaagggttt cgccaacgcc gagcatgcgc gtatctcctg ggaggttgtc
     1741 tgaaatggat acggcgagga gggggagccc ctagccggtg acctccagat ggtccctcgc
     1801 acctgcgaag tcaaatcgat catttcgcac ttgcgaatag actgaggtta tgaaggccgg
     1861 ctcactcaga aggccaatcg tcataagttc agttgttagc gacatcaaaa tctatcgatt
     1921 gtctctttca acctgcgggg cataggctcg agctaacgat cataaatctg gaggaacgac
     1981 catggacggc gaacaggatc ccacacccct gaacgacccc aaaaaggagc ccgcagcgct
     2041 gcggaccctg ctgggccgca acaatcgcga ctggtggcca aaccacctgt ccatggacat
     2101 ccttcatcag cagggtcgat cgggcgatcc gatgggcgac ggcttcaact acggcgaggc
     2161 gttcaaaaag ctcgattacg ctgctgtgaa gcgcgacctt accgccctga tgaccgacag
     2221 tcagccctgg tggccggcgg actatggcca ctatggcccc ttcttcattc gcatggcctg
     2281 gcactcggcc ggtacctatc gcaccggcga cgggcgcggc ggcgccagcg cgggccagca
     2341 gcgtttcgct cctctgaaca gctggccgga caacggcaac ctcgacaagg cgcggcgcct
     2401 gctgtggccg atcaagcaaa aatacggcgc gaacctgtcg tgggccgatc tgatgatcct
     2461 ggccggcaac gtcgcgatcg agtcgatggg cggcccggtg ttcggctttg gcggcggtcg
     2521 tgccgacgtc tgggagcccg agaaggacgt ctactggggc accgaagaag agtggctggg
     2581 cgaaacccgt atcgacgagg agtccggcaa ggccctcgag gcgccgttgg cggcgatcca
     2641 gatgggcctg atctacgtca accccgaagg cccgggcggt aacgcccagg cgctgcagtc
     2701 tgcccgcgat attcgtgaga ccttctcgcg gatgggcatg aacgatgagg agacggtggc
     2761 cttgaccgcc ggtggtcaca cgttcggcaa ggcccacggg gcgggcgacg cctccaaggt
     2821 cggttccgat cccgaaggcg ccgatatcgc ccagcaaggg ttaggctggc agtccggcca
     2881 cgagagcggc atgggcgacc atacgattac ctcgggactt gagggcgcat ggaccccgac
     2941 gccgacgacc tgggacatga ccttcttcga catgctgctg gaccacgagt acgaactggt
     3001 ccgcagcccg gccggcgcga agcagtggca gcccgtcggc aaccccgaag acaccttggc
     3061 tccgggtgcg cacagcccgc accgcaaggt gccgaccatg atgaccaccg ccgacatggc
     3121 gctgaaggtc gatcccgcct accgcgcgat ctgcgagaaa ttccgctccg atccggccta
     3181 tttcggcgac gccttcgccc gcgcctggtt caagctgacg caccgcgaca tgggaccgaa
     3241 gtcccgctac ctcggcccgg aagtgcctgc cgaagagctg atctggcagg acccgattcc
     3301 gcggcggaca gggcccgcca ttggcgatgc ggacatccgg gtgctgaagg atgcgattgc
     3361 agcctcgggc ctgtccgtag ccgaactggt gaagacggcc tgggcctcgg ctgcgaccta
     3421 tcgcggctcg gaccatcgcg gcggcgccaa cggcggccgg ttgcggctcg agccccagcg
     3481 cagttgggag gtcaatgagc cggaccagtt ggcgcgcgtg cttcaggtct atgagggcat
     3541 caaggccggg tcggggacga acgtctcgat cgccgatctg atcgtgctcg gcggctcggt
     3601 cgggatcgag caagcggctc aggcggccgg tcatgcgatc gaggttccct tcacgccggg
     3661 ccgtaccgac gccagcgccg aacagacgga tgttgagggc ttcgccgttc tggagcccaa
     3721 ggcggacggc ttccgcaact atctgtcggt gcgcttcaac gtgccgaccg aggagctgct
     3781 ggtcgaccgg gcccaacttc tgggtctgac cgcgcctgag atggcggtgc tggtcggcgg
     3841 cctgcgcgta ctgggcgcca accacggcgg aacgccgcac ggcgttctga ccaaacgtcc
     3901 gggccagttg accaatgact tcttcgtcaa cctgctcgac atgacgaccg cgtggaaaga
     3961 ggtcgacgat cgcggtgacg aggtcttcct cggcacggac cgtatcaccg gcgaagagct
     4021 gtggaccgcg acccggaccg atctggtgtt cggctccaac tcgcaactgc gcgccctgtc
     4081 cgaggtctac gcctcggcgg atgcaggcga gaagttcgtc cgcgacttcg ccaactcctg
     4141 ggccaaggtg atgaacgccg accggttcga cgtcaccctc gaggaagagt tcggcacggc
     4201 tgcggtcgac gcctgacgat tgggttgggc ggtcccctgc ggcacagcca ttggggacct
     4261 cccatagcct gactaaatga tcgtggcttt ctccatagtg agcacctatg tggctcacat
     4321 gctccccaca ctgcgacagc tccagtatct caagctcttg gccgaacacg cctcgttcag
     4381 ccgcgccgcc gaggccgccc acgtcagcca gcctgcgcta tccgcaggag tgcaggagct
     4441 cgagaagatc ctgggcgctc cggtcgttga gcgcacacgc ggcaacgtcc agctgactgc
     4501 cgttggtatc gaggccgtca aacgcgccga agacgtgctg gcccgcaccg aggatctggt
     4561 cgaggcggcg cgtagtgcag gacggcccct gtcgggccgg ttccggctcg gcatcattcc
     4621 gaccgtggcg cccttcctgc tgcccgccac tctgccggcc ctgaagacgg cctatcccgc
     4681 gctgcgcctg ttcatccgcg aagacctgac gcctcgactg atcgcgggcc tgaaagccgg
     4741 ccagctcgac gccgccgtca tcgccctgcc gtatgacgcg ccggggatcg agcacgccca
     4801 catcagtgac gacgagatcg tggcggcggc ccctgccggc cacgccttgg ccgcgccggg
     4861 cgcaatccag tcgggctcgc tcaaggccga ggacctgatc ctgctagagg acggtcactg
     4921 cctgcgcgat caggcgctgg ccgccttcga catcgaagcg cccaagggcg acgacgtctt
     4981 cgccgccacc agcctgcata cgctggttca gatggtcagt tcgggacttg gcgtatcctt
     5041 cctgccccag atggcggtca aggcggggct cgccgacacg ccgtcggtgg tcatccgcca
     5101 catcacgccc cgggcaggtg cgctcgctcc aagccgtgag atcgtggtcg cctggcgcgc
     5161 cggttccagc cgcgccgccg aggcccggct gctggccgag gcgatgagcc aggactgact
     5221 ggtcgaagat cgatgtcgca acggaaaacg gctctcaagc gttgccgctg cgaactgtca
     5281 ggatcgaacc tcatgcgcct ctctcgctca atcgccgtcg ccgctctgat cggtggcgct
     5341 gccgccctgt ccggctgcgt cagcatgtcc cgatcaaagt ccgtctcggc tttggatccg
     5401 gcccttgctt ctggtgcgcg cgtgtccgag attcgcctga ccaccgacga caaggtcacc
     5461 gtgcgccccg agttcgccgg catcttccgt gaccacgtgc agaagaaact ggatggttgc
     5521 gccaccggcg ctcaacccct gcggcttgag gccacgatca gccggttcga ccgcgccaat
     5581 ccggctcagg tcatgctgat cggcggcgcc aacgtcctgc gcggccacgc ccgtctggtc
     5641 gatcccgcga cgggccgcgt cgtggcggaa tacgagatcg gtcgcaccat catgggcagc
     5701 cgcttcggca tcttccagat ggccgagagc gaggaacaat tgtccgacgc cttcggcgcc
     5761 gagctctgcg atcaggcctt caaggctgac tgattgcctc agcgcatgat cgagcccggc
     5821 agggcaagaa cgacgatggc gcagaacagg ctggtcatgc cggtcaggaa tatccggtcg
     5881 gcccagattt ccaaccgtgc gcttgctcgg ccgttcccga tcgacagata ggacaggaag
     5941 cacgccagca ggaaacacac tgcgcccaac catgcgatct cgtcggctaa cgactgcgcc
     6001 gccttatcgg tcagatgcag tcctgcgatg atcagcaggg tgatgccgag taggttcgac
     6061 gagacgttga gaatgtgcgg cgagcggttc atggctcagc tttcggctcg aaaaggggcg
     6121 ttttgtagaa gcggctcacc cgggaatgac agccgtcgtg tagggcgagt ggagccccca
     6181 cccgagcgtg cgataaaggg cttggccttc ctctgtggcg acgagaacgg ctttgcgcgg
     6241 cccccctgca gcggtctcca gcgtgcgcat cacctggcga cccaatccga gccgccgatg
     6301 atcggggtcg acggcgatcc ggtcatagat gacccggtcg tcgacaatca caaaccgccc
     6361 cctcgctgcc tcagcgccgg aagcgtgccg gatggtgcag aaggtcaccg cgccgtcttg
     6421 ccagaggctc gtcgtataac cttgcggtgg aggaggacct tcggacatcg cgccgtccaa
     6481 cgtcattaga aaccccgatg ggcgcaacga ccatgtattg ggcagaatgg cgcgcacctc
     6541 ctgtgcatct gcacagacct tgagaaagat gcagggctcc gagattgctg ctgcacggcg
     6601 agccacggcg tcatcggcct tggcgaagac atagcgtcgc aactgatcgt cgagcccgac
     6661 ctcgacccgc cagccgccgt cgtcccacga gggtggggaa acgccgcgcg tcagggccca
     6721 gcctcgggcc cagatcgcca acaggtccgg atgtgcgctc atgccgagag cgtgaccctt
     6781 tcggtcgcac gcggcaacgc cttcggcaac ggtttgcggg gtgtgacgtt gtttcgcctc
     6841 gccgaaggag ccgcgtaatg aacggggctt catcgcagcg ccagaatatg gtctggcttg
     6901 ggtcataacg acgccagcct ccctgatcag attggcgtca tcgaaaaccc atcccgaacg
     6961 ggaggaagat tctatccatg accgtttccc gccgcgcctt cgcttttgga accgccgcca
     7021 tcgccgggct ggccgccctg ccggctgccg cgcagacctc gctgtcgtcc gaggaccgtg
     7081 cgacgctgca gacggcccag acctatttgc agaacctgag ttcggctcag gggacgttcg
     7141 tcgagaccgg gccaggcggc cagcaacgtc gtggccgttt ttatctgcag cgcccgggca
     7201 agatgcggtt cgaatacacc gatcctgcgg gcctgctggt cgtctcggat ggctacaacg
     7261 tcaagcgcta cgatccccgg ctcgaggtct tccgtcaggt gccgctgggg gcgacgcctc
     7321 tgtcgacctt cctcgctcgc aacgtccgcc tcgatcaggg cgtacgcatc gatcgcgtga
     7381 cccgcatgca gtcgggcgcg tttgcgctga cggtgcggga cagcgccaag ccgaatgagg
     7441 gttcggtgat cctggccttc gccggcagcc cactgaggct gcaggaatgg acaatcacgg
     7501 acgcacaggg cggtcgaacg cggacccaac tgaccacgct acagcccgct tcgggtctgg
     7561 cggcgagcct gtttcagctg cgcgacccga cgcgtcgtca gggccgcaac tagtcgcccc
     7621 ccaccgctat atcggcgtgc ggacgattcg atgaacgtgg ttatgccgca acggaaaagc
     7681 ttcatagttt agtatttgac cttttttctt ctgcgtgaca ctttgggcac agaacggcgg
     7741 cggtcgttct tccgcgtcgg acatcatccc ctcccgattg cggcgtgtga gagagaaaaa
     7801 cttcagcgcc cggcacgctc gtgccgggcg ttttttctcg ctagaagccg ggcatgaccc
     7861 tgcgtatcgc cacctggaac ataaactccg tccgtctgcg catcgagcag gtcgcgcggt
     7921 tcgtgaccga gagcgaaacc gacgtcctgt gcctgcagga gatcaaatgc ctcgaggacc
     7981 agtttccgcg gaacgccttt gccgaaatgg gcatgccgta tctgcggatc gcggggcaga
     8041 agggctggca tggggtggcg atcgccagcc gtcgtccgat catcgatgcg ccagcgctgg
     8101 acgtctgtcg caacaagcat gcgcgctgcg tctcggccct ggtcgagggg gtcgagatcc
     8161 agaatttcta catccccgcc ggtggtgacc tgccggaccg ggagttgaac gaaaagttcg
     8221 atcacaagat ggattattac gcgcgactga ccgccgagat ggctgcgcgc gaccagttgg
     8281 cgcccttgct gctggcgggc gacttcaaca tcgcgccgag cgagttcgac gtctggaacc
     8341 atcgctatat gtccagggtc gtcagtcata cgccgggcga ggtcgagacc ctgaaccggc
     8401 tacaggcggc gggcggcttc agcgacgtgg tccgtgacgc ctatccggag ccgcagaagc
     8461 tggcgagctg gtggagctat cgcgcgcagg actttcgcaa gtcgaaccgg ggtctgcgcc
     8521 tcgaccacct gtggacctcg cccggcctga cgccgaaggt tgtcccgggc agcgccacga
     8581 tccacgacac cgtgcgcgaa tggacccagc cgagcgacca ttgcccgatc accgtcgacc
     8641 tcgacgtcta gcgctcagta gacgggatct gcgttgaggg ggctgcgcgt cacgaacttc
     8701 tggcgcccca gctttccgta ggccttggcc agacgctcca cggcgagatc aagctcggct
     8761 tcaggcaggg tgtagggcag acgcaggcgg ttttcgaacg cgccgtccac gccgaagcga
     8821 ggccccgccg ccagccgcaa gccctcgatc gccgcctgca ccgtcagggc cgaactgatc
     8881 ggtcccggca ggcgcgccca gatcgatagt ccgccgtccg ggcggggaca gacccagtcc
     8941 gggaatcgct cggccaaccg cgccagcagg tgatcgcggc gggtcctgag cagcttccgc
     9001 ctcaccgaaa gcgccgcacc cccgtc
//
LOCUS       DASVTM010000034         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_960123,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000034 DASVTM010000000
VERSION     DASVTM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960123"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF633_00770"
     CDS             complement(<1..304)
                     /locus_tag="VF633_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX8469621.1"
                     /translation="MAAVQETIDAVAALEKYEHGFTSEIETEYAPRGLSADIVRFISE
                     KKGEPEWMLEWRLAAYERWLAMEEPTWASVKYEPVDYQSLFYYAAPKKKNGPKSLDE"
     gene            complement(304..1440)
                     /locus_tag="VF633_00775"
     CDS             complement(304..1440)
                     /locus_tag="VF633_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX8469622.1"
                     /translation="MSVYLDYNASGLIRPEVLEAVNATLTDGGNPSAIHALGRKARAR
                     IETARAQVASLVGADATAVIFSSGGTESNGQGLASAFAAGCDRLIVGATEHPCILEAA
                     VASGKPVKMLPVDANGVISLGKLSELLRAPGKAVVAVHHANNESGVIQPIADAARLVR
                     EAGGWLHVDAVQSAGKIPVDMRTLGADSLTLSAHKLGGPQGVGALVMSEGRSAVAVIH
                     GAGQERGMRGGTENVAGVTGFGVAAQSSLRDLHLAEAHRPWRDSAERTVVEAGAVIIG
                     AGADRLPNTLFMAVADWDSPQQIITLDLAGIMASGGSACSSGKTKPSRAILATGRTDL
                     AIGGVRASGGWTTTEDDWIRFASTWTEAYARQRERAAARKREVA"
     gene            complement(1437..1943)
                     /locus_tag="VF633_00780"
     CDS             complement(1437..1943)
                     /locus_tag="VF633_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8469623.1"
                     /translation="MRLSTKGRYAVMAMADLAKNSLAENGAGRAVSLAEIAMRQEISL
                     SYLEQLFARLRKGGLVMSVRGPGGGYRLAKAAGDTVVADIVLAVDEPIRATRCVGHSS
                     PKGCMMAGERCITHNLWEDLGDEIHRYLAGVSLEDVVLNRTGARRSVGQVEAGLLSAE
                     LLSVGAPA"
     gene            2102..2752
                     /locus_tag="VF633_00785"
     CDS             2102..2752
                     /locus_tag="VF633_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8469624.1"
                     /translation="MPEVILPGASGRIEGRYSPGKRANAPIALILHPHPKAGGHMNNP
                     VTVTLHQLFQQRGFATLRYNSRGVGKSQGEFDSGIGELADAATALDWLQSNNPAATQT
                     WVGGYQFGAYIGMQLLMRRPETDGFISVSPPSNMYDFSFLAPCPASGLFLHGTADTIV
                     PPVEVERVVNKLRTQKGIVIDYELEEGASHFWQNHMDAVDRRVGLYLDKRLEENPA"
     gene            complement(2814..4016)
                     /locus_tag="VF633_00790"
     CDS             complement(2814..4016)
                     /locus_tag="VF633_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX8469625.1"
                     /translation="MKFGIDRLISEPALQAELKGKRVALLAHPASVTADLTHSIDALA
                     ALGNVRLTAAFGPQHGMKGDLQDNMMETPDETDPVHGIPVFSLYGEVRRPSAEAMATF
                     DVILIDLQDVGCRIYTFVTTLLYVLEAAAEHGKTVWVLDRPNPAGRPIEGLTLRPGYE
                     SFVGAGPMPMRHGLTLGEMGLWFTDHFKLDVDYRVIEMQGWQPEQAPGYGWPLSERSW
                     INPSPNAPNVSMVRAYAGTVMLEGATLSEGRGTTRPLELFGAPDIVARDVIAEMRRLA
                     PEWLHGCVLRDCWFQPTFHKHVCQLCNGIQMHVDDPHYDHAAFKPWRVQALAFKAVRN
                     LYPDYDLWRDFPYEYASGKLPIDTINGGPALREWVDDRSATAADLDALTLPDEAAWEE
                     ARKPFLLY"
     gene            complement(4013..4534)
                     /locus_tag="VF633_00795"
     CDS             complement(4013..4534)
                     /locus_tag="VF633_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEX8469626.1"
                     /translation="MHASLIPHPTTANGPIGRIQVQMAREGGQLWLRFTAEGEVDRVL
                     WPAQATPGRANDLWRHTCFEVFVRTPEGYREFNLSPSGKWASYAFDGYRDGMRDASGV
                     ATVEPLDGASDQVALEARIQLPPDASAFGLSAVIEDVDGGISYWALAHPSDKPDFHHP
                     DSFTLELPAPEPA"
BASE COUNT          808 a         1524 c         1531 g          800 t
ORIGIN      
        1 cctcgtcgag cgacttgggt ccgttcttct tcttgggagc ggcgtagtag aacagcgact
       61 ggtagtcgac cggctcgtat ttcaccgagg cccaggtcgg ttcttccatg gcaagccagc
      121 gctcgtaggc ggcgagacgc cattcaagca tccactcagg ttcgcccttc ttctcactga
      181 taaagcggac gatatcggcg ctgagcccgc gcggcgcgta ctccgtctcg atctcggacg
      241 tgaacccgtg ctcatacttt tccagcgccg cgacggcgtc gatggtttct tgaacggcgg
      301 ccattatgcg acctccctct tgcgtgcggc ggcccgttcg cgctggcggg cgtaagcctc
      361 ggtccaggtc gatgcgaaac ggatccaatc atcttcagtg gtggtccagc cgcccgaggc
      421 gcgaacacct ccgatggcca gatcggtgcg gccggtggcg aggatggcgc gcgacggctt
      481 ggtcttgccc gacgaacagg ccgacccgcc ggacgccatg atcccggcga gatcgagcgt
      541 gatgatctgc tgggggctgt cccagtcggc gacggccatg aacagggtgt tgggcaggcg
      601 gtcggcgcca gcaccgatga tcacagcgcc ggcctcgacg acggttctct cggcggagtc
      661 gcgccaaggg cgatgagcct cggcgagatg caggtcacgc agcgacgatt gcgcggccac
      721 gccgaagccg gtgacgccag cgacgttttc ggtgccgccg cgcatcccac gttcctgacc
      781 tgcgccatgg atgaccgcga ctgcggatcg cccctcgctc atcaccaaag cgcctacgcc
      841 ttgcgggccg cccagcttgt gagccgacaa cgtcaggctg tcggcgccga gtgtgcgcat
      901 atcgaccggg atcttgcccg ccgactggac cgcgtcgacg tgcaaccagc caccggcctc
      961 gcgcaccaga cgggcggcgt cggcgatggg ttggatgacg ccgctctcgt tgttggcgtg
     1021 atggacggcg accacggcct tgccgggcgc gcgcagcagt tcggacagct tgcccagcga
     1081 gatcacgccg ttggcgtcga ccggcagcat cttgaccggc ttgcccgagg cgacagcggc
     1141 ttcgaggatg cagggatgtt cggtggcgcc tacgatcagc cggtcgcaac ccgctgcaaa
     1201 ggcgctggcc agcccctgac cattggactc cgtgccgccg gacgagaaga tcaccgccgt
     1261 cgcatcggcg ccgacgaggc tggcgacctg ggcccgagcc gtctcgatac gcgcgcgcgc
     1321 cttgcggccc agagcgtgga tggcggacgg gttgccgccg tcggtcaggg tcgcgttcac
     1381 ggcctccagc acttccggac ggatcagacc cgaggcgttg tagtcgagat agacgctcac
     1441 gccggagccc ctactgaaag cagttcagcc gagagcaaac cggcctcgac ttgcccgacc
     1501 gaacggcgcg cgccggtgcg attcaggacg acatcctcga gcgagacgcc ggcaagatag
     1561 cgatggatct cgtcaccgag atcttcccac agattatggg tgatgcagcg ctcgccggcc
     1621 atcatgcagc ccttgggcga agaatggccg acgcagcggg tcgcgcggat cggttcatcg
     1681 accgccaaca cgatatcggc gaccacggtg tcacctgcgg ccttggccag ccgatagccg
     1741 ccgcccggtc cacgcacgct catcaccagt ccgcccttgc gaagacgggc gaacagctgt
     1801 tcgaggtaac tcaatgaaat ctcttggcgc atggcgattt cggccagcga aacggcacgg
     1861 cccgcaccgt tctcggcgag gctattcttg gccagatcgg ccatcgccat cacggcgtat
     1921 cgtcccttgg tcgacagtcg catcgcacac cttcaaaatt ctcgcgcttt tcaggggtcc
     1981 tgtgctaaga cccgcgcctt ctgaacagcg gcggcgcgca aggtggactt agaaagtcct
     2041 accccagcgg tcaagtattc atgccgttga aacgacatat gtgaacggat tccagagccc
     2101 catgcctgaa gtcatcctgc ccggcgcctc cggtcgtatc gaaggtcgct attccccggg
     2161 caagcgcgcc aatgctccga tcgccttgat cctgcacccg caccccaagg ccggtggcca
     2221 catgaacaac ccggtgacag tgacgctgca ccagctgttc cagcagcgtg gcttcgccac
     2281 cctgcgctac aattcgcgcg gcgtcggcaa gtcgcagggt gagttcgaca gcggcatcgg
     2341 cgagctggcc gacgccgcca cggcgctcga ctggctgcag tcgaacaacc ccgccgcgac
     2401 gcagacctgg gtcggcggct atcagttcgg cgcgtatatc ggcatgcaac tgctgatgcg
     2461 ccgccccgag acggacggct tcatatcggt gtcgccgccg tcgaacatgt acgacttcag
     2521 cttcctggcg ccctgcccgg cctcgggcct gttcctgcac ggcacggccg acaccatcgt
     2581 cccgccggtc gaggtcgagc gcgtggtcaa caagctgcgc acccagaagg gcatcgtcat
     2641 cgactatgag ctggaagagg gcgcgagcca cttctggcag aaccacatgg acgccgttga
     2701 ccgccgcgtg ggcctgtatc tggacaagcg cctggaagaa aacccggcct aggctgcgct
     2761 gttcggaaag cttcagaacc tgacatcctc tcccggcggg agaggggtca aagtcagtaa
     2821 agcaggaacg gctttctcgc ctcttcccat gcggcttcgt cgggcagcgt cagggcgtcc
     2881 agatcggcgg cggtggcgct ccggtcgtcg acccattccc gcagcgccgg gccgccgttg
     2941 atggtgtcga tcgggagttt gccggaggcg tattcgtacg ggaagtcgcg ccacaggtcg
     3001 tagtcgggat agaggttgcg cacggccttg aaggccagcg cctggacccg ccagggcttg
     3061 aaggcggcgt gatcgtagtg cggatcgtcg acgtgcatct ggatcccgtt gcacagctgg
     3121 cagacgtgct tgtggaaggt cggctggaac cagcagtcgc gcagtacgca gccgtggagc
     3181 cactccgggg ccagtcgacg catctcggcg atgacgtcgc gggcgacgat gtcgggggcg
     3241 ccgaacagtt ccaacggccg ggtggtgccg cgtccctcgg acagggtcgc accttccagc
     3301 atgacggtgc cggcataggc gcggaccatg gagacattgg gggcgttagg gctggggttg
     3361 atccagctgc gctcgctcag cggccagccg taaccggggg cttgttcggg ctgccacccc
     3421 tgcatctcga tgacgcggta atcgacgtcg agcttgaagt ggtcggtgaa ccacaggccc
     3481 atctcgccca gcgtcaggcc gtgacgcatc ggcatgggac cggcgccgac gaaactttca
     3541 tagccggggc gcagggtcag gccctcgatg gggcgtccag cggggttggg acggtccagc
     3601 acccagaccg tcttgccatg ctcggcggcg gcctccagca catagagcag cgtcgtcacg
     3661 aaggtataaa ttcggcaacc gacatcctgc aggtcgatca ggatgacgtc gaaggtggcc
     3721 atcgcctcgg ccgagggtcg gcgcacctcg ccatacaggc tgaagacggg gatgccgtgg
     3781 accggatcgg tctcgtccgg cgtctccatc atattgtcct gcaggtcgcc cttcatcccg
     3841 tgttgagggc cgaaggcggc ggtcagtctg acattcccca aggctgccag cgcgtcgatg
     3901 gaatgggtca ggtcagccgt caccgaagcc ggatgggcca gcagggcgac gcgtttgccc
     3961 ttcagctcgg cctgcagcgc gggttcggag atcaggcggt cgataccgaa tttcatgcag
     4021 gctccggcgc tggaagctcc agcgtaaatg agtcgggatg gtggaagtcg ggcttatccg
     4081 aaggatgcgc caaggcccaa tagctgatgc ctccgtcaac gtcctcgatg actgcggaga
     4141 ggccgaatgc ggatgcgtct ggcggcaact ggatacgcgc ctccagggcg acctgatcgc
     4201 tggcgccgtc cagcggctcg accgtggcga ccccactggc gtccctcatg ccgtcgcggt
     4261 agccgtcgaa cgcgtaggac gcccacttgc ccgagggtga caggttgaac tcacggtagc
     4321 cctccggcgt ccggacgaag acttcgaaac aggtgtgtcg ccagagatcg ttcgcccgtc
     4381 ctggcgtcgc ctgagccggc cagagcacgc gatcgacctc gccctccgcc gtgaaccgta
     4441 gccacagctg accaccttca cgggccatct gcacctgaat gcggccaatg ggaccgttcg
     4501 ctgtggtggg atgtgggatg agggaggcgt gcatccacaa ccgatagcgc tcgcttgacg
     4561 ccggtcaact gtgacggcta ctcaagcgtc atgttcgcca cccgcaccct cacaggtctg
     4621 tattaccgct cgctgagcta gcgagcggct gcgaactcac gcg
//
LOCUS       DASVTM010000035        12740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_588160,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000035 DASVTM010000000
VERSION     DASVTM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588160"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..161)
                     /locus_tag="VF633_00800"
     tRNA            complement(88..161)
                     /locus_tag="VF633_00800"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(127..129),aa:Gly,seq:tcc)
     gene            complement(248..331)
                     /locus_tag="VF633_00805"
     tRNA            complement(248..331)
                     /locus_tag="VF633_00805"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:Tyr,seq:gta)
     gene            478..1368
                     /gene="rlmB"
                     /locus_tag="VF633_00810"
     CDS             478..1368
                     /gene="rlmB"
                     /locus_tag="VF633_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX8469627.1"
                     /translation="MSRNPERNDRKAGKKPDSGNRPAAGSDGPRKGGWNPNPPAKSFA
                     DSTPRAPARAKEGADNFVWGRHPVLAALANPARKGMGRLLATEDRAAELERDKLNHGH
                     TIEVIDSQLLERMLPVGAVHQGLAFKVTPLDGVPLDELADPAEGIIVMLDQLTDPQNV
                     GAIFRSALAFGARGIVVQDRHSPALAGALAKASAGATERLPCARVTNLSRALETLADL
                     GWRAVGLDGNSEETLESVLDARPTVLVMGSEGEGIRRLVAEHCDVLAKIPMPGGFESL
                     NVSNAAAVALYVATQKQAKA"
     gene            1414..2205
                     /locus_tag="VF633_00815"
     CDS             1414..2205
                     /locus_tag="VF633_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX8469628.1"
                     /translation="MESLFTPLLLSQLAALGQVLMIDLVLAGDNAVAVGLAAAALPQE
                     QRRKAILVGLGFAVVLRIVFALVTVQLMAVVGLLLAGGVLLLWVCWKMWRELREAATH
                     DQAEAQRELELAASIEHGTGPSPEELGLKRKTFGAALLQILIADVTMSLDNVLAVAGA
                     AHEHPWILAGGLIISIALMGLAATYIARLLNRFRWIGYVGLVIVLLVAGRMIWDGGRS
                     VVVRTGNMDEFNASAPAFLDINEEEAEEYLRGMKQPEATAAETAP"
     gene            complement(2251..2997)
                     /locus_tag="VF633_00820"
     CDS             complement(2251..2997)
                     /locus_tag="VF633_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEX8469629.1"
                     /translation="MTTGEPQVWTPGSGLAAQAPDRIDAARDRLARTGRTQLHYLLDD
                     RYARHLKSEAEAVDYNVVTLRGTGHVDLPAAWLASLEPRHKEGLGQAIQAHATGQFQY
                     LYDNHPIYDLNAAGRAAPVWSDLVSFLSGAPFLNLMREVTGEPRIAQADAQLTRFRPG
                     HFLTTHDDHAEGKSRYFAYVLGLSEGWRIDWGGLLAFHDAEGNVAEAFTPRFNTLNLL
                     KVPQPHSVTQVALSAGANRISITGWLRGAG"
     gene            complement(2994..4694)
                     /gene="metG"
                     /locus_tag="VF633_00825"
     CDS             complement(2994..4694)
                     /gene="metG"
                     /locus_tag="VF633_00825"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX8469630.1"
                     /translation="MARILITSALPYINGIKHLGNLAGSMLPADVWARFKRGQGHEVL
                     YICATDEHGTPAELAAAAAGQDVRTYCDEQHQIQKSAGEAFGLSYDHFGRSSNPPNHR
                     LTQHFAEALERNGLIEERVDRMIYSIDDARFLPDRYVEGTCPHCAYPKARGDQCDNCG
                     RLLDPIDLIDPYSSVSGSKNLEVRDTRHLYLLQTKLEQPIRDWIASKTDWQTLAKSIA
                     YKHLDEGLIDRGITRDLAWGIPVVKDGFPRPGMEDKVFYVWFDAPIEYIGATQEWAEK
                     NGTTWRDWWRLDEGAEDVRYVQFMGKDNVAFHTVSFPATILGSGEPWKTVDTLKAFNW
                     LNWYGGKFSTSQKRGVFMDQALELLPADYWRWHLTAYGPEGSDAAFTWEQFQSTTNKD
                     LADVLGNFVNRIVKFTESKFGGVVPAGGEAGPVEAKLIEDVEAGIAEATAAFEAMEFR
                     KAAQALRAVWVLGNEYLQVAAPWTAFKTDPVQAAVGVRTGLNLVALFARLAAPVMPFS
                     AVKIAASVGASDLRWPAPGEDFLNALPLGQPVQAAEVLFKKIEDAQVVEWTERFGGSE
                     "
     gene            complement(4747..6084)
                     /locus_tag="VF633_00830"
     CDS             complement(4747..6084)
                     /locus_tag="VF633_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8469631.1"
                     /translation="MLSPATRTATKDLLGLAWPVILARLGIMTMGLTDVIVVGNYSAR
                     ELAFSSLALAPMSIVVTTAVGLMMGVQVMTARFLGEGRRQEVGAVFRRGVSYSLQLGV
                     GAMLVLLLLGPLGLKHAGLADGLGEGASPALRVFALSMPGYLVSVAAQFFLEALHKPK
                     PGMWAMWVANGINLALNLLLVPDILGLGVHGAEASAWATFAARTSLAVFLIFYIIRLP
                     EARELGVFLKPNRDPLAEREQRKVGYGAGSSYFIEVGAFAAMTFIAGQLGAIETSAWA
                     IVLNVSAIVFMVPMGLSAATAVLVGRAYGARDGRGVLRAGMVGIGVVTVMTVIVALGL
                     WAFAPVVASAYNRDPLLLAVVAPALVLATLFFVADGIQVVSASANRAAGDVWWPTIMH
                     FGAYTLVMMPVGWVLAHSLGVDGLVWAVIVASLVSSVLLTGRFVRVARRLPQG"
     gene            6185..6796
                     /locus_tag="VF633_00835"
     CDS             6185..6796
                     /locus_tag="VF633_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEX8469632.1"
                     /translation="MTLRPLFVTQVYEASLAPTPNFDVFNSELAEACRMLAYEDGAGR
                     AWCKSHGYRGYTSYGSLNDLPNRIPEFAELKRLLDRHALAYAKGLNFDLARKPRLDNL
                     WVNILKPGGGHSGHIHPHAFLSGTVYIEVPDGASALKLEDPRLPMMMARPGVVADAPD
                     AEKPFVYLAPQAGTVLMWESWLRHEVPPNAAKAERISISFNYA"
     gene            6873..7436
                     /locus_tag="VF633_00840"
     CDS             6873..7436
                     /locus_tag="VF633_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8469633.1"
                     /translation="MTDREAHLKALMLRGLDGDAVAWRELLTDMGAHLRPYFRTRLFD
                     GGADAEDLVQETLIAIHAKRGTWDPAQPFTGWAYAIARHKLIDHLRRQGRRPTHPLEE
                     ASALFAEHTVEDGATRADLSRCLSVLPARQRKLIEDVRLKGLSVAEAAERHGYTLTAA
                     KVAIHRSLKSLNAKFAPAPATESVDED"
     gene            7426..8061
                     /locus_tag="VF633_00845"
     CDS             7426..8061
                     /locus_tag="VF633_00845"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1109 domain-containing protein"
                     /protein_id="HEX8469634.1"
                     /translation="MKTDDLIQALSLDLPPTQQLDRRLMLAMIPAALVVLITVGLWLG
                     YRDDLMLAMRGPVFWAKAAYSVLMAGVGFWLLERLGRPGASPRAPLILLAAVLALALG
                     LALLQLIAMPMPERMPAMMGESAHACAPTIALLSLVAAPFIFWAARAFAPTRPMLAGA
                     AAGLLTAGMATTLYGLHCHEHAAAFVALWYTLGMAIVAAAGALIGKLAFRW"
     gene            complement(8062..8691)
                     /locus_tag="VF633_00850"
     CDS             complement(8062..8691)
                     /locus_tag="VF633_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8469635.1"
                     /translation="MSLSSDAAPVVSRGRPRSFDRQVALDQAMHVFWKKGFGGASMSD
                     LTAAMGIASPSLYAAFGSKEGLYREAIEHYVVLHADEFWGVMNAPTARESIEGLLRNA
                     ARAFTECDAASGCLVMQTAAETGDLSADLEASLCDLRASGAAQFAERLRRGIAEGDVA
                     AGTNVRGVADFYATIHKGMSLSAKGGAGREELDSVVTSAMAAWDPLTGG"
     gene            8806..9558
                     /locus_tag="VF633_00855"
     CDS             8806..9558
                     /locus_tag="VF633_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8469636.1"
                     /translation="MTSPLRLAGKRALVTGAARGIGAAIALKLAEDGADVAITYEKSA
                     EKAEALAVEIRALGRRAVAIQADAASPQAVKDAVAQTVAELGGLDILINNAGVGGSGG
                     LVADESLEDIDHLLNVNVRGVFLTTQAALAHIPDGGRIISIGSCLGDRVSFPGVTIYS
                     ATKSALESFNRGLARELGSRQITVNLVKPGPIDTDMNPADGDHADGVRASVALGHYGE
                     TRDIAEAVAFLAGSEAKYITGTSIVVDGGSNA"
     gene            10021..11688
                     /gene="ettA"
                     /locus_tag="VF633_00860"
     CDS             10021..11688
                     /gene="ettA"
                     /locus_tag="VF633_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX8469637.1"
                     /translation="MAQQYIFQMQGLTKAFPGGKKIFENIWLSFYNDAKIGVVGVNGS
                     GKSTLLKIMAGIDPEFSGEAKAADGIKRGYLEQEPQLDDSLNVRENVEAWCEEKQWVN
                     RFNAIAGEMAEEYTDELMEEMTKLQEKIDAGDVWDIDSRIEMAMDALRCPPDDWSTEN
                     LSGGEKRRVALARLLLSKPDMLLLDEPTNHLDAESVAWLQHHLEAFPGCVILVTHDRY
                     FLDLVTKWTLELDRGKGIPYEGNYSSWLEQKQKRVVQENSESEARQRALTRELEWVRS
                     GAKARQAKSKARLAAYERMVDEQDQQRGAQTHAVIQIPPGPRLGNVVLEVEGLQKSYG
                     EKLLFDNLTFKLPPNGIVGVIGPNGAGKSTMFRMITGQETPDGGTIRVGETVKLAYVD
                     QSRDSLDPNKTIWEEISGGTDVMMVGKREINTRAYVGSFNFKGGDQQKKVGQLSGGER
                     NRVHLAKTLATGGNLILLDEPTNDLDIETLQNLEEALEDFAGCAVVISHDRWFLDRLA
                     THILAFEGDSHVEWFEGNFEAYEEDKKRRLGTDALIPHRIKFQKFGR"
     gene            11782..12711
                     /locus_tag="VF633_00865"
     CDS             11782..12711
                     /locus_tag="VF633_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX8469638.1"
                     /translation="MTTRPAVLIMQRHLGQLTPFLERAYDVYRIWEGPPTEVEACIEA
                     IIVAGEFELDKALIERLPNLKLIACFTSGYDRIDVEWCRARGLPVTHAPGVNHEDVAD
                     HAIGLIIAARRQIVAGDRALRAGSWTPETRILTPSLKDQKVGIVGLGLIGQAIAARAE
                     AMRMSVRWWGPRNKPADWPRAASLLELAKDSDILVVACRADDDNIGLISREVIEALGP
                     SGLLVNVSRGQVLDEDAVIAALKSGALGQAALDVFTEEPVDPAIWADVPNTVLTPHTA
                     GATTEAVQGMLMLLLQNLSAVFAGEPLKTPVQD"
BASE COUNT         2335 a         4285 c         3939 g         2181 t
ORIGIN      
        1 tgcggcttgt tacgttcgaa cttttccttg gccatggcca aactcctgat ggcccgggtt
       61 gaggggcgct gtgtgcattc aaaaacctgg agcgggtaga cgggatcgaa ccgacatcct
      121 cagcttggaa ggctgctgca ctaccattgt gctatacccg cgcggagacc gcctcggacc
      181 ctccccgtaa ggcggggcct cggcgaccgg tccggttcgt cgtcctgatt gtcgtttcga
      241 aagcgcgtgg tgggggaagc aggactcgaa cctgcgaagc ttacgcaggg gatttacagt
      301 ccccccccgt tgccactcgg gacattcccc cagcgcgttt tcgaaccgtt caaaccgtct
      361 tgtcggtccc ctttcggagg ccggcgcaac aaaagccttc agtattctga cacgcctgag
      421 atagaggggg cgccagaccc gaaagcccta gggctccaaa tcggagggcg tcttatagtg
      481 tcgagaaatc cagaacgcaa cgatcggaaa gcaggcaaaa aacctgattc cggaaacagg
      541 cctgcagcgg ggtccgacgg gccacgcaaa gggggctgga atcccaatcc cccggcgaaa
      601 agcttcgccg attcaacacc ccgggctcca gcccgggcca aggaaggcgc ggacaatttc
      661 gtctggggcc gtcacccggt gctcgccgca ctggcgaatc cggcccgaaa aggcatgggt
      721 cgcctgctcg cgaccgaaga tcgggcagcc gagcttgagc gtgacaaact gaatcacggt
      781 cacacgatcg aggtcatcga ttcccagctt ctggaacgga tgctgcctgt cggcgccgtc
      841 catcagggcc tggccttcaa ggtgacgccg ctggacggcg ttcctctcga tgagctcgcg
      901 gatcccgcag aggggatcat cgtcatgctg gatcagttga ccgatcccca aaacgtgggc
      961 gccatcttcc gttcggccct cgccttcggg gcgcgcggca tcgtcgtgca ggaccggcac
     1021 tcgcccgccc tcgccggcgc tctggccaag gcctcggccg gcgccacgga acgcctgccc
     1081 tgtgctcgcg taacgaacct cagccgggcg ctcgaaactc tggccgatct gggctggcgc
     1141 gcggtcggcc tcgacgggaa ctcggaagag acgttggaaa gcgttctcga tgcccgcccg
     1201 acggtgttgg tcatggggtc ggagggcgaa ggtatccgcc gtctggtcgc cgaacactgt
     1261 gacgtgctgg ccaagatccc gatgccgggc ggcttcgaaa gcctgaacgt atcgaacgcg
     1321 gctgcggtgg cgctttatgt ggcgacacaa aagcaagcca aggcctgaca gagaggggtt
     1381 cacgtagcac tgtctcgccg ctagagaccg cttatggaat ccctcttcac gccccttctt
     1441 ctcagccagc tcgccgccct cgggcaggtg ctgatgattg acctcgtcct ggccggtgac
     1501 aacgccgtcg ccgtgggact ggccgccgcc gccctgccgc aggaacaacg ccgcaaggcg
     1561 atcctcgttg gcctcggctt tgcggtggtg ttgaggatcg tcttcgccct ggtcaccgtg
     1621 caactgatgg ctgtcgtcgg cctgctgctg gcgggcggcg tcctgctgct gtgggtgtgc
     1681 tggaagatgt ggcgcgagct gcgcgaggcc gccacccacg atcaggccga agcccagcgt
     1741 gaactggaac tcgccgcctc gatcgagcac ggcaccggcc cttcccccga ggaactgggc
     1801 ctgaagcgca agaccttcgg tgcggccctg ctgcagatcc tgatcgccga cgtgaccatg
     1861 tcgctcgaca acgtcctcgc cgtcgccggc gctgcgcatg agcatccgtg gatcctcgcc
     1921 ggtgggttga tcatctccat cgccctgatg ggcctcgccg ccacctacat cgcccgcctg
     1981 ctgaaccgct tccgctggat cggctatgtc ggtctggtca tcgtcctgct ggtcgccggt
     2041 cgcatgatct gggacggcgg ccgttcggtc gtggtccgca ccggcaacat ggatgagttc
     2101 aacgcctccg cccccgcctt cctcgacatc aacgaggagg aagcggagga gtatttgcgt
     2161 gggatgaagc agcccgaggc gactgcggcc gagacggctc cctgagctga tcggcatcgt
     2221 ggtcactgat caacggtagg actgacgccc ctagccggct ccgcgcagcc agccggtaat
     2281 cgatatcctg ttcgccccgg ccgacagggc gacttgcgtc acagagtgcg gctgcggcac
     2341 cttcagcagg ttcaaggtgt tgaaccgggg cgtgaacgct tcggcgacat tgccctcggc
     2401 atcatgaaac gcgagcaggc cgccccaatc gatcctccag ccttcgctca gacccagcac
     2461 ataggcaaag tagcggctct tgccctcggc atggtcgtcg tgggtggtca ggaagtggcc
     2521 ggggcgaaat cgcgtgagct gagcatccgc ctgagcgatc cttggctcgc cggtcacttc
     2581 gcgcatcagg ttcagaaacg gcgccccact cagaaacgac accaaatctg accagaccgg
     2641 cgccgcccga cccgctgcgt tcaggtcata gatcgggtga ttatcgtaca ggtattggaa
     2701 ctgcccggtg gcatgagcct ggatcgcctg accgaggccc tccttatgtc gaggctcaag
     2761 cgaggcgagc cacgccgccg gcaagtcgac atgccccgtg ccgcgaaggg tcaccacgtt
     2821 gtaatcgacg gcttcggcct cggacttcag atggcgagcg tatcgatcgt ccagaaggta
     2881 atgcaattgc gtccgtccag tccgcgccag ccggtctcgc gccgcatcga tccggtctgg
     2941 cgcttgcgcc gccagaccgg atccgggcgt ccatacctga ggctcgcctg tcgtcactcg
     3001 gacccgccga agcgctccgt ccactcgacc acctgagcgt cctcgatctt cttgaacaga
     3061 acctcggcgg cctgcacggg ctggccgagc ggcaaggcgt tcaggaagtc ctctcccggc
     3121 gccggccatc tcaggtcgct cgcgccgacg ctggcggcga tcttcaccgc cgagaacggc
     3181 atgaccggtg cggccaaacg ggcgaacagg gccaccagat tgaggccggt gcgaacgccc
     3241 acagccgcct gaaccggatc cgtcttgaac gcggtccacg gcgcggcgac ctgcaggtac
     3301 tcgttaccca gcacccagac ggcgcgcagg gcctgagccg ccttgcggaa ctccatcgcc
     3361 tcgaaggcgg cggtggcttc ggcgatgccc gcctcgacgt cctcgatcaa ctttgcttcg
     3421 accggcccag cctcgccgcc cgcgggcacg acgccgccga acttcgattc cgtgaacttg
     3481 acgatccggt tgacgaaatt gcccagcaca tcggccagat ccttgttcgt cgtcgactgg
     3541 aactgctccc aggtgaaggc cgcgtccgag ccttcgggac cataggccgt caggtgccag
     3601 cgccagtagt cggcgggcag cagctcaagc gcctgatcca tgaagacgcc gcgcttttgc
     3661 gaggtcgaga atttgccgcc gtaccagttc agccagttga aggccttcag cgtgtcgacg
     3721 gtcttccacg gctcgcccga gccgaggatg gtcgcgggga agctgacggt gtggaaggcg
     3781 acgttgtcct tgcccatgaa ctggacgtag cggacgtcct cggccccttc gtccagacgc
     3841 caccagtcgc gccaggtcgt gccgttcttc tcggcccatt cctgcgtcgc gccgatgtat
     3901 tcgatggggg cgtcgaacca gacgtagaag accttgtcct ccatgcccgg gcgcggaaag
     3961 ccgtccttga ccaccgggat gccccaggcg aggtcgcgcg tgatgccgcg atcgatcagc
     4021 ccttcgtcga ggtgcttgta ggcgatcgat ttcgccagcg tctgccagtc ggtcttcgac
     4081 gcgatccagt cgcggatcgg ctgttccagc ttcgtctgca gcagatagag gtggcgtgtg
     4141 tcgcgcacct cgaggttctt cgatcccgag accgacgaat agggatcgat caggtcgata
     4201 ggatccagca accggccgca gttgtcacac tggtccccgc gcgccttggg ataggcgcag
     4261 tgcggacacg tcccctcgac gtagcggtcc ggcaggaagc gcgcgtcgtc gatcgaatag
     4321 atcatccggt cgacgcgttc ctcaatcagg ccgttccgct ccagcgcctc ggcgaaatgc
     4381 tgggtcaggc gatggttcgg tgggttcgag gaccggccga aatgatcgta ggacaggccg
     4441 aacgcctcgc ccgcagactt ctggatctga tgctgttcgt cgcaataggt gcggacgtcc
     4501 tgaccggcgg cggctgcggc cagctcggcc ggggtgccat gctcgtcggt ggcgcagata
     4561 tacagcacct catggccctg cccgcgcttg aaccgcgccc agacgtcggc cggaagcatc
     4621 gagcccgcga gattccccag atgcttgatg ccgttgatgt acggcagggc cgaggtaatc
     4681 aggatgcggg ccatgggaat ccagtaagct tgagagcggt ggatatagac accggatggc
     4741 tccccgtcac ccttgcggca gccgcctcgc cactctgacg aaccgtccgg tcaacagaac
     4801 cgaggacaca aggctggcga cgatcactgc ccagaccaga ccgtcgacgc ccagactgtg
     4861 agccagaacc cagcccaccg gcatcatcac cagcgtatag gcgccgaaat gcatgatggt
     4921 cggccaccag acgtcgcccg ccgcccggtt ggccgaggcc gaaacgacct gaattccgtc
     4981 ggcgacgaag aacaatgtcg ccagcaccag cgccggggcc accaccgcca gcagcagcgg
     5041 atcgcggttg taggcgctgg ccaccaccgg cgcgaacgcc cacagcccta gcgccacgat
     5101 caccgtcatg accgtgacga cgccgatccc gaccatcccg gctcgcagca cgccgcgtcc
     5161 gtcgcgcgcg ccatacgctc gaccgaccag taccgccgtc gccgccgaca gccccatcgg
     5221 gaccataaag acgatggccg agacattgag cacaatggcc caggccgagg tttcgatcgc
     5281 gccaagctgg ccggcgatga aggtcatggc ggcgaaggcc ccgacctcga tgaaatagct
     5341 ggaccctgcg ccatagccca ccttgcgctg ctcgcgttcg gccagcggat cacggttggg
     5401 cttcaggaac acacccagct ctcgcgcctc tggcaggcgg atgatgtaga aaatcaggaa
     5461 caccgccagc gatgtccgcg ccgcgaaggt cgcccacgcc gaggcctcgg cgccgtgcac
     5521 gcccagcccc agaatgtccg gcactagcaa caggttgagc gcgagattga taccgttggc
     5581 gacccacatg gcccacatac ccggcttggg cttgtgcagg gcctcgagga agaactgcgc
     5641 cgcgacgctg accagatagc cgggcatcga cagggcgaag acgcgcagcg ccggcgacgc
     5701 cccctccccc agaccatcgg ccagaccggc atgcttcagc cccagcggcc cgagcagcag
     5761 caaaaccagc atggcgccga cgccgagttg cagcgagtag gacaccccgc gcctgaagac
     5821 agcgccaact tcctgtcgcc gtccttctcc gaggaagcgc gcggtcatca cctgcacgcc
     5881 catcataagg cccaccgcag tggtcacgac gatgctcatg ggggcgagcg caagagagct
     5941 gaacgccagc tcacgcgccg aatagttacc caccacgata acgtccgtca gacccatggt
     6001 catgatgccc agacgcgcca ggatcaccgg ccaggcgagg cctaggaggt ctttggtggc
     6061 ggtacgggtt gcggggctca acatgggacg cggcacggac cacgggacgg ccacggggtc
     6121 aacacttggg ctgcttatgg gttgataagg tgacgggcgt gcgcgtgctc gctagagaac
     6181 cccgatgacc cttcgccccc tcttcgtcac ccaggtctat gaggcctccc tcgctccaac
     6241 gccgaacttc gacgtattca actccgaact ggccgaagcc tgccgtatgc tggcctacga
     6301 ggacggcgcc ggccgcgcct ggtgcaagtc acacggctat cgcggctaca cgtcgtatgg
     6361 ctcgctcaac gacctgccca accggattcc cgagttcgca gagctgaagc gacttctgga
     6421 ccgccacgcc ctcgcctatg ccaaagggct gaatttcgac ctcgcgagga aaccccgtct
     6481 cgacaatctc tgggtcaata ttctgaagcc cggcggcggc cactccggcc atatccaccc
     6541 ccacgccttc ctgtccggca cagtctatat cgaggtcccg gacggcgcct cggcgctgaa
     6601 gctggaggac ccccgcctgc cgatgatgat ggcccgcccc ggcgtcgtcg ccgacgcacc
     6661 cgacgccgag aagcccttcg tctatctggc gccacaggcc ggcaccgtgc tgatgtggga
     6721 aagctggttg cgccatgagg tgccgccgaa cgccgccaag gccgaacgca tctcgatcag
     6781 cttcaactac gcctgagttt ccaaggccta gatggcgtca tgctgcaggc cgtgtaacct
     6841 tccccacgca acggacgaag acatccccga ccgtgacgga tcgtgaagcc catctgaaag
     6901 cgctgatgct ccggggtctc gacggcgacg ccgtggcctg gcgcgaactg ctgaccgata
     6961 tgggcgcgca tctgcggccg tatttccgga cccgcctgtt cgatggcggt gccgatgctg
     7021 aggatctcgt gcaggaaacc ctgatcgcca tccacgccaa gcgcggcaca tgggatccgg
     7081 ctcagccctt caccggctgg gcctatgcga tcgcgcgtca caagctgatc gaccatctgc
     7141 gccgccaagg ccgtcgcccc acccatccgc tggaagaggc ctcggccctg ttcgcggaac
     7201 acaccgtcga ggacggcgcg accagagcgg atttaagccg gtgcctgtcc gtgctgcctg
     7261 ctcgccaacg caagctaatc gaggatgtgc gtctgaaagg cctctccgtc gccgaggccg
     7321 cagaacgcca cggttacacg ctgaccgccg ccaaggtcgc gattcaccgc agcctgaaat
     7381 cgctcaacgc gaagttcgcg cccgcgcccg cgacggagtc cgtcgatgaa gactgacgac
     7441 ctgatccagg ccctgtccct cgatctgccg ccgacgcagc aactcgaccg gcgactgatg
     7501 ttggcgatga tccccgccgc cttggtggtg ttgatcacag tcggtctttg gctcggctat
     7561 cgcgacgacc tgatgctggc gatgcgcggt ccggtgttct gggccaaagc cgcctattcc
     7621 gtcttgatgg caggcgtcgg cttctggctg ctggaacgat tgggccgtcc cggggccagt
     7681 cctcgcgcac cattgatcct gctggccgca gttctggcgc tggccctggg cttggccctg
     7741 ctccaactga tcgcgatgcc catgcccgag cggatgcccg ccatgatggg cgagagcgct
     7801 cacgcctgtg cgcccaccat cgccttgctc agcctcgtcg ccgcgccctt catcttctgg
     7861 gccgcgcgag ccttcgcgcc cacccgcccc atgctggccg gcgcggcggc gggtctcctg
     7921 accgcaggta tggcgacaac cctctacgga ctgcattgcc acgagcacgc cgccgccttc
     7981 gtggccctct ggtacacgct cgggatggct atcgttgccg cagccggcgc cttgatcgga
     8041 aagctggcct tccgctggtg atcacccgcc agtgagtggg tcccacgccg ccatcgccga
     8101 ggtcaccacc gaatccagct cttcccgtcc agccccgccc ttggcgctga ggctcatgcc
     8161 tttatggatg gtggcgtaga agtcggcgac gcccctgacg ttcgtcccgg cggcaacatc
     8221 accttccgcg atcccacgtc gtaagcgctc ggcgaattgc gcggcgccgc tcgcgcggag
     8281 atcacagagg ctggcttcca gatcggccga taagtctcca gtttctgcgg ccgtctgcat
     8341 caccagacaa ccgcttgccg catcgcattc ggtgaaggcg cgcgcggcat tccgcaacag
     8401 accttcaatg ctttcgcgcg cggtcggcgc gttcatcacg ccccagaact catccgcgtg
     8461 gagcacgacg taatgttcga tggcttcgcg atagaggcct tccttggacc cgaaggccgc
     8521 atagaggctg ggcgacgcga tgcccatggc cgccgtcagg tcgctcatag aagcgccgcc
     8581 aaagcccttc ttccagaaca catgcatcgc ctgatcgagc gcgacctgac gatcaaaact
     8641 gcgggggcgg ccccggctga cgacgggagc cgcgtcggat gacagcgaca ttcggacctc
     8701 ttctttgctg atcgatacat aatcccttgc gccgggacgg caagcctccc agttagttct
     8761 gtgccaatcg atacagatct ggcagaactc gaaggattac ccagaatgac ctcccctctc
     8821 cgccttgccg gcaaacgcgc cctcgtcacc ggcgcagcac gcggcatcgg cgcagccatc
     8881 gccctgaagc tcgccgaaga tggcgctgac gtcgccatca cctatgaaaa gtccgctgaa
     8941 aaggccgaag ctctcgcggt cgaaatccgc gctctgggtc gcagggctgt tgccattcaa
     9001 gcggacgccg ccagcccgca ggctgtgaag gatgccgtag cacagaccgt agctgaactt
     9061 ggtggtctcg acatcttgat caacaacgct ggcgtcggtg gtagtggtgg actggtggcc
     9121 gacgagtcgc tcgaagacat cgaccatctg ctgaacgtca acgtccgcgg cgtcttcctg
     9181 accacccagg cagccttggc tcacattcct gacggcggcc ggatcatctc gatcggttcg
     9241 tgcctcggtg accgcgtctc tttccccggt gtgacaatct attcggcgac caagtcggcg
     9301 ctggaaagct tcaaccgtgg actggctcgt gaactcggct cgcgtcagat cacggtcaat
     9361 ctggtcaagc ccggcccgat cgacacagac atgaaccccg ctgacggcga ccatgcggac
     9421 ggtgtccggg cctccgttgc actgggccac tacggtgaaa cccgcgacat cgccgaagcc
     9481 gtcgccttcc tcgccggatc ggaagcgaaa tacatcacgg gcacgagcat cgtcgtcgac
     9541 ggtggctcga acgcctgatg cggcgggttt ccggcggcga tctggccccg caagggcggt
     9601 tcgtcgccgg agactctcga ccctgatatt gtggagacat ccgacccccg gaagggagac
     9661 tctcgacccc gggataaagg gccggtcgaa atcgacgcac catgctccga ggctgagcta
     9721 gaatatgagt ccggacttcc tttggtcgtc cagagcccac ggtctttgac attgtcagca
     9781 tgaaacactc tccgggagga ggcgtcttct ccaggaattg cgtgcgccca caccggtcgg
     9841 cgcacgtaac cgatcctgag gtcatcgatt ttttcccgga atggatacac tggacccgaa
     9901 cggtcactcg ccatcaaacc gccctccccg tcgggtagtt catttgccac ccggcgccgg
     9961 gatcgctaca tcccgcgcct acctcccaat ccgttcagac gacagggcgc accgcaactc
    10021 atggcgcagc aatatatttt ccagatgcag ggcctcacca aggcctttcc cggcggcaag
    10081 aagatcttcg agaacatctg gctgagcttc tacaacgacg ccaagatcgg agtcgttggc
    10141 gtcaacggtt cgggtaaatc gaccctgctg aagatcatgg ccggcatcga ccccgagttc
    10201 tcgggcgagg ccaaggccgc cgacggcatc aagcgcggct atctggagca ggagccccag
    10261 ctcgacgaca gcctgaacgt ccgcgagaac gtcgaggcct ggtgcgaaga gaagcagtgg
    10321 gtcaaccgct tcaacgcgat cgccggtgag atggcggaag aatacaccga cgaactcatg
    10381 gaggagatga ccaagctcca ggagaagatc gacgccggtg acgtctggga catcgacagc
    10441 cgcatcgaaa tggccatgga cgccctgcgc tgtccgcctg acgactggtc gaccgagaac
    10501 ctgtccggtg gtgaaaagcg ccgtgtggct ctggcccgcc tgctgctgtc caagcccgac
    10561 atgctgctgc tcgacgaacc gaccaaccac ctcgacgccg aatccgtcgc ctggttgcag
    10621 caccacctcg aagccttccc cggctgcgtc atcctggtga cccatgaccg ttacttcctc
    10681 gacctcgtga ccaagtggac gcttgagctc gatcgcggca agggcatccc ctacgaaggc
    10741 aactactcca gctggctgga gcagaagcag aagcgcgtcg tgcaggagaa ctcggaatcc
    10801 gaagctcgcc agcgcgccct tacccgcgaa ctggaatggg tccgttcggg cgccaaggcc
    10861 cgtcaggcca agtccaaggc ccgtctggcc gcctatgagc ggatggtcga cgagcaggac
    10921 caacagcgcg gcgcccagac ccatgccgtg atccagatcc cgcccggacc gcgcctcggc
    10981 aacgtcgtgc tggaggtcga aggcctgcag aagtcgtatg gcgagaagct gctgttcgac
    11041 aacctgacct tcaagctgcc gcccaacggc atcgtcggcg tcatcggccc caacggcgcc
    11101 ggcaagtcga ccatgttccg catgatcacc ggccaggaaa cgcccgacgg cggcacaatc
    11161 cgcgtcggtg aaacggtcaa gctggcctac gtcgaccagt cgcgcgactc gctcgatccc
    11221 aacaagacga tctgggaaga gatctcgggc ggcaccgacg tgatgatggt cggcaagcgc
    11281 gagatcaaca cccgcgccta tgtcggctcg ttcaacttca agggcggcga tcagcagaag
    11341 aaggtcggtc agttgtccgg tggtgagcgc aaccgcgtcc acctcgccaa gacgctggcg
    11401 accggcggca acctgatcct gctcgatgaa ccgaccaacg acctggacat cgagacgctg
    11461 cagaacctcg aggaggctct ggaggatttc gcgggctgcg ccgtggtcat ctcccacgac
    11521 cgctggttcc ttgaccgtct ggccacccac atcctcgcct tcgaaggcga cagccacgtc
    11581 gaatggttcg agggcaactt cgaagcctac gaagaagaca agaagcgccg ccttggcacc
    11641 gatgccctga tcccccaccg gatcaagttc cagaagttcg gtcgctaagc ctacggcaga
    11701 ttgcgcagtc aacgacctcc cctgcgggag gtcgttttcg tttgggatga cgcccgaagc
    11761 cggtcggcgt agtctggttc catgaccacg cgtcccgccg ttctgatcat gcagcgccat
    11821 ctgggacagc tgacgccgtt cctggagcgc gcctacgacg tctatcgcat ctgggaaggg
    11881 cctccgaccg aggtcgaggc gtgcatcgag gccatcatcg tcgctggcga gttcgagctc
    11941 gacaaggcgc tgatcgaacg gctgccgaac ctcaagctga tcgcctgttt cacctcgggc
    12001 tatgaccgga tcgatgtgga gtggtgtcgg gcgcggggcc tgccggtcac ccacgcgccg
    12061 ggggtcaatc acgaggatgt cgccgaccac gcgatcggcc tgatcatcgc cgcacgacgc
    12121 cagatcgtcg ccggggaccg cgccctgcgg gccgggagct ggacgccaga gacccgaata
    12181 ctcacgccgt cattgaagga ccagaaggtc gggatcgtcg gtctcggcct gatcggtcag
    12241 gcgatcgcgg ctcgtgccga ggcgatgcgg atgtcggttc gctggtgggg tccgcgaaac
    12301 aagcccgccg actggcctcg cgctgcgtcg ctgctggaac tggctaagga tagcgacatc
    12361 ctcgtcgtcg cttgccgggc cgacgacgac aacatcggcc tgatctcgag ggaggtcatc
    12421 gaggcgctcg gcccgtctgg tttgttggtc aatgtctcgc gcggtcaggt gctcgacgaa
    12481 gacgccgtga tcgcggcctt gaaatcagga gcgctgggcc aggccgcgct ggatgtcttc
    12541 accgaggagc cggtcgatcc agcgatctgg gcggacgttc cgaatacggt gctgacgccc
    12601 cacaccgccg gagcgacgac cgaagcagta cagggcatgc tgatgctgct gctgcagaac
    12661 ctgtcggcgg tcttcgcagg cgaaccgttg aagacccccg ttcaggactg aaggctagtt
    12721 gccgtcgggc atttgcatgc
//
LOCUS       DASVTM010000036         5866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_482576,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000036 DASVTM010000000
VERSION     DASVTM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482576"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /gene="lepA"
                     /locus_tag="VF633_00870"
     CDS             <1..1262
                     /gene="lepA"
                     /locus_tag="VF633_00870"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX8469639.1"
                     /translation="RLPAPKGDANAPLKALLVDAWYDPYLGVVVLVRVFDGVLKAGMR
                     VKMMQNGSTHLIDRVGVFLPKNTPVDQLGPGEVGFITAQIKEVADAAVGDTITDERKP
                     TTTALKGFKQVQSVVFCGLFPVDAADFEDLRAAIGRLRLNDASFTFEMESSAALGFGF
                     RCGFLGLLHLEIIQERLSREFNLDLIATAPSVVYKIQLRDGAEIDLHNPADLPDVMAI
                     ETISEPWIKATILTPDEFLGGVIKLCQDRRGSQVELSYVGSRALVVYELPLNEVVFDF
                     YDRLKSISKGYASFDYELTDYKVGDLVKMSILVNAEPVDALSMLVHRARAETRGRGMV
                     EKMKELIPPHMFVIPIQAAIGGKIIARETVRALRKDVTSKCYGGDATRKKKLLEKQKA
                     GKKKMRQFGKVEIPQEAFIAALKMDDD"
     gene            1351..2361
                     /locus_tag="VF633_00875"
     CDS             1351..2361
                     /locus_tag="VF633_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX8469640.1"
                     /translation="MSSTSDVRRDLPVWDEKHLRLGIAAAGIALWAWNVDSDGLTMDD
                     IGYDLWAVAKDQSVTFEDLSANIHPADRDRVRVAFNATRGIVGPYEIDFRIIVKDEVR
                     WISARGRGDDEGIVGRVMFGVFLDVTGRKQAEEAHELLAGEMSHRVKNLIAIAGALTT
                     MTSRSAETKEDMARELTHRLTALGRAHDLVRPVPGEIETAALLGDLLSVLLTPYDDLG
                     AFSGRIRVSVPRMAIGESAATNLALVLHELATNSVKHGALSVDTGLLDVACLVDDGAA
                     VLVWTERGGPRVVPPVPSKGFGSQLLKRIMTHHFAGSVTYDWAPDGLVATLKIPAGRL
                     AV"
     gene            complement(2525..3613)
                     /locus_tag="VF633_00880"
     CDS             complement(2525..3613)
                     /locus_tag="VF633_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8469641.1"
                     /translation="MKPSVRLLAAVTFMAAASPVWAQQAPAVPAPAGTSSDQGGTGRT
                     RPVVAVFGDNVGSEAVDFLTPYGVLSSSGVAEVVAVSLEAGPVHLQPALTIEADETVA
                     SFDARFPQGADFVIVPAISRRNDPAVIAWLQQQAAKGATLVSICDGAWTLAATGLLDG
                     HRATAHWASIKGLKKAYPQVDWVRDQRWVHDGKVQSTTGVSAALPASLALVEQIGGRG
                     AAERTARLYGADGWSAAYDSSPFRLTAGVASTAVWNMVAVWGHEKVGVRVVPGVDEAV
                     LALTMDPLARTYRTQAVTVAATALPVPTAHGLTIIPDQSEAETSPRMTFVVPSATAPM
                     DSALAWIGARYGKGTRRLVELELEAPSQ"
     gene            3776..4837
                     /locus_tag="VF633_00885"
     CDS             3776..4837
                     /locus_tag="VF633_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8469642.1"
                     /translation="MTLSTPAFISARAQSSAGSTTPAVRRVMMIAFPRSQILDVVGPL
                     EVLGRVAALVPGGGYEIELVASAPVQGSDAITTTSGLQLQAQRRLDEVPDAAIDGLDT
                     LIVAGGAGAVEACSDTRLIDFVKRTSGRIRRTTAICTGAFVLGEAGVLDGRRAVTHWS
                     HLDDFRTRYPQVSLDADAIYVRDVRPDAGEVWSSAGVTAGIDLTLAMVRDDFGAAVAL
                     AIARDLVMFMVRPGGQAQFSAQLVAQAAEGKRTARAVAHILDHPCDDLGVAALAHLSN
                     MSERTFARVFRQELGMTPALFVERARVDTARRLLEADDVPVKQLFGLSGFRDEETLRR
                     AFQRQLGVNPSAYQARFSG"
     gene            complement(5116..>5866)
                     /locus_tag="VF633_00890"
     CDS             complement(5116..>5866)
                     /locus_tag="VF633_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEX8469643.1"
                     /translation="NVSAALAELGLPHIRGYRPMANYQRALFPALERYLDTHPNAWML
                     GQSIFAPTRARGPQSRETTNSNPGFAEASEGYSGTLTPEPRASDIVLPTDPPPFLTPR
                     KPRPEGLVRLVRKYDPAKRDNANRLLGRLGEQHVLEHEIARLVAADRMDLAKKVEWTS
                     DVHGDGAGYDIRSFEPDGSDRLIEVKATRGGPTTDFFLTRTEREVSEEQPDAWRLYRL
                     HTLPISPRLFVLPPPLEASVRLEAENWRAAF"
BASE COUNT          987 a         1928 c         1944 g         1007 t
ORIGIN      
        1 cccgcctgcc cgcgcccaag ggcgacgcca acgcgccgct gaaggccctg ctggtcgacg
       61 cctggtacga cccctatctg ggcgtcgtgg tgctggtgcg ggtgttcgac ggcgtgctga
      121 aggccgggat gcgcgtcaag atgatgcaga acggctcgac ccacctgatc gaccgcgtcg
      181 gcgtcttcct gcccaagaac acgccggtgg accagctcgg ccccggcgag gtcggcttca
      241 tcaccgccca gatcaaggaa gtcgcggacg ccgccgtcgg cgacactatc actgacgaac
      301 gcaaacccac gaccacggcg ctcaagggct tcaaacaggt ccagtcggtg gtcttctgcg
      361 gcctgttccc ggtcgacgcc gccgatttcg aggacctgcg cgccgccatc ggccgcctgc
      421 gcctgaacga cgcctccttc accttcgaga tggagtcctc ggccgcgctg ggcttcggct
      481 tccgctgcgg cttcctcggc ctgctgcatc tggagatcat tcaggagcgc ctcagccgcg
      541 agttcaacct cgacctgatc gcgaccgccc cctcggtggt ctacaagatc cagctgcgcg
      601 acggtgccga gatcgacctg cacaacccgg ccgacctgcc cgacgtgatg gcgatcgaga
      661 ccatctccga gccgtggatc aaggccacca tcctgacccc cgacgagttc ctcggcggcg
      721 tcatcaagct gtgtcaggac cgtcgcggct cgcaggtcga gctgtcctac gtcggttcgc
      781 gcgcgctggt ggtctatgag ctgccgctga acgaggtcgt gttcgacttc tacgaccggc
      841 tcaaatccat ctccaagggc tatgcctcgt tcgactatga gctgaccgac tacaaggtcg
      901 gcgatctggt caagatgagc atcctcgtca acgccgagcc cgtcgacgcc ctgtcgatgc
      961 tggtccaccg cgcccgcgcc gagacgcgcg gccggggcat ggtcgagaag atgaaggagc
     1021 tgatcccgcc ccacatgttc gtcatcccga tccaggccgc catcggcggc aagatcatcg
     1081 cgcgcgagac ggtgcgcgcg ttgcggaagg acgtgaccag caaatgctac ggcggcgacg
     1141 cgacccgcaa gaagaagctg ctggagaagc agaaggccgg caagaagaag atgcgtcagt
     1201 tcggcaaggt cgagatcccg caggaagcct tcatcgcggc gctgaagatg gacgacgatt
     1261 gagcgcccgg gagatgcaca gttcagcttg ccgttcgagc gggaataggg ctctgctttc
     1321 cggccccgcc tccgagaagg acgtttcaat atgagctcga cgtccgatgt ccgccgggac
     1381 ttgcctgttt gggacgaaaa acacctgcgc ctcggtatcg cggcggcggg catcgccctg
     1441 tgggcctgga acgtggacag cgacgggctg acgatggacg acatcggcta cgacctgtgg
     1501 gcggtggcca aggaccagag cgtcaccttc gaggacctgt cggccaacat ccaccccgcc
     1561 gatcgggatc gcgtccgcgt cgccttcaat gccacgcggg ggatcgtcgg cccttatgag
     1621 atcgacttcc gcattatcgt caaagacgag gtgcgctgga tttcagcgcg tggccgcggc
     1681 gacgacgagg gtatcgtcgg tcgtgtcatg tttggcgtct ttctggacgt gacgggccgc
     1741 aaacaggccg aggaggccca tgaactgctg gccggagaaa tgagccaccg ggtcaagaac
     1801 ctgatcgcca tcgccggcgc cctgaccacc atgacgtccc gctcggcgga aaccaaagag
     1861 gacatggcgc gcgagctgac gcaccgcctg acggctctgg gccgggccca cgatctggtg
     1921 cgccccgtcc ccggcgagat cgagaccgcc gcccttctgg gcgatctgct gtcggtcctg
     1981 ttgacgccct atgacgacct cggcgccttc agcgggcgta tccgcgtctc ggtgccgcgc
     2041 atggcgatcg gcgagagcgc cgcgaccaat ctggcgctgg tgctgcatga actggccacc
     2101 aactcggtca agcacggcgc cttgtcggtc gatacgggtc tgctcgacgt cgcctgtctg
     2161 gtcgacgacg gcgccgcggt ccttgtctgg accgagcgcg gcgggccgag ggtcgtgccg
     2221 cccgtcccgt ccaagggctt cggcagtcag ttgctgaaac ggatcatgac gcaccatttt
     2281 gccgggtcgg tcacctacga ctgggccccc gacggtctgg tcgcgaccct gaagataccg
     2341 gcggggcggc tcgccgtctg atcgggttta cgattgggag cgcaccgcgc ccgatcgttt
     2401 cctgcatgtg gtccgcgacg gtctgaagcc ccgcagcccg acgccgtccg gccgcccgca
     2461 ggagccgttg ccggcgccgg gggaggtgga cgggtgacgg gccatgcgcc ggagcagccg
     2521 tgcttcactg cgacggcgcc tccagttcca gttcgaccag acgacgcgtc cccttgccgt
     2581 agcgggcgcc aatccatgcc agcgcggagt ccatcggcgc tgtcgccgac gggacgacga
     2641 aggtcatgcg aggcgaggtc tctgcttcgc tttgatccgg gatgatcgtc agcccatgcg
     2701 cggtgggcac cggcagggcc gtcgcggcga cggtcacggc ctgcgtccga taggtccgcg
     2761 ccagtggatc catcgtcagc gccagtacgg cctcgtcgac cccggggacg acccgaacgc
     2821 ccactttctc atgtccccag accgcgacca tgttccagac ggcggtagag gccacgcctg
     2881 cagtcaggcg aaacgggctg ctgtcatagg ccgctgacca gccgtcggcg ccatagagcc
     2941 gggcggtgcg ttcggccgcg ccacggccgc cgatctgttc cacgagggcg agagacgcag
     3001 ggagggcggc cgacactccg gtcgtcgact gcacttttcc atcatgcacc cagcgttggt
     3061 cgcgcaccca gtcgacctgc ggataggcct tcttcagtcc cttgatcgag gcccagtggg
     3121 ccgtggcccg gtgaccgtcc agcagtccgg tcgcagccag ggtccatgcc ccgtcgcaga
     3181 tagaaaccag cgtcgccccc ttggccgcct gttgctgcaa ccaggcgatc acggccggat
     3241 cattgcgccg tgagattgcg ggaacgatca cgaaatcagc gccttgcgga aagcgggcgt
     3301 caaaactggc gaccgtttca tcggcctcga tggtcagcgc gggctgaaga tgcaccggcc
     3361 cggcttcgag cgataccgcc acgacctcgg ccacgccgga gctggagagg acgccgtacg
     3421 gcgtgaggaa gtcgaccgct tccgatccga cattgtcacc gaagacggcc acgacaggcc
     3481 gcgtccgacc ggttccgcct tggtcagaag acgtccctgc aggcgccggg acggcgggcg
     3541 cctgttgggc ccagacgggc gaagctgcgg ccataaaggt gacggcggcg agaaggcgaa
     3601 ctgaaggctt catgatgggg ttccaatgag acctgcggag ggtcagaccc ggacggtctc
     3661 gtcacaagga caagcgcccc tcggaaacgg ccaatgcgaa gatcccccac gcgatggcgg
     3721 aatccgaggg ttgaaggtcc ttttcgcgaa cggcgtcagg gcgcaaagcg gtcggatgac
     3781 gctttccact ccggctttca tcagcgcgcg cgcccagtca tccgcaggtt cgaccacgcc
     3841 cgccgtccgg cgcgtgatga tgatcgcctt tccccggtcc cagatactgg acgtcgtggg
     3901 acctcttgag gttctgggac gcgtggccgc cctcgtgccc ggcgggggtt atgagatcga
     3961 actggtcgcc tccgcgccag tgcagggaag cgacgcgatc accacgactt cgggtctcca
     4021 gcttcaggct cagcgtcgtc tggatgaggt gcccgatgcc gccatcgatg gactggacac
     4081 cctgatcgtg gcgggcggcg ccggcgcggt cgaggcctgt tccgacaccc gtttgatcga
     4141 cttcgtcaaa cggaccagtg gccgaatccg ccgcaccacc gccatctgca ccggggcctt
     4201 cgtgctcgga gaagccggtg tgctggacgg gcgccgcgcc gtgacgcact ggagccatct
     4261 cgacgatttc cgcacccgat acccgcaggt gtcgctggac gccgacgcca tctatgtgcg
     4321 cgacgtcagg cccgatgcgg gcgaggtctg gagctcggcc ggagttacgg cgggcatcga
     4381 cctgacgctg gccatggtcc gcgacgactt cggcgccgcc gtcgccctcg ccatcgcgcg
     4441 cgatctggtg atgttcatgg tgcgccccgg cggacaggcc cagttttccg cccagcttgt
     4501 ggctcaggcg gcggagggca agcgcacggc gcgcgccgtg gcccacatcc tcgaccaccc
     4561 gtgcgacgat ctgggtgtcg ccgctctggc ccatctgtcc aatatgtcgg agcgcacctt
     4621 cgcccgcgtc tttcgtcagg agctgggcat gacccccgcc ttgttcgtcg agcgcgcccg
     4681 cgtcgacacc gcccgtcgtc tgcttgaggc tgacgacgta cccgtcaaac aactattcgg
     4741 cctgagcggc ttccgcgacg aggaaaccct gcgccgcgcg tttcagcggc agttgggggt
     4801 gaacccgtca gcgtatcagg cgaggttctc ggggtgaggg ggggcgtttt accaaccttc
     4861 ggttggtagc ggctggccca gattgcgccc aacctcgcag gtgatggcat ccttaagcgc
     4921 aacctccaaa gaacgtgctg gctgggtggc ggaggtcgat cgtctgatga agcgcgactg
     4981 gtgcatcgac acagcggatg ctggactcag cgccgaggac tccgagcgct tttggcgcga
     5041 cggcgaaaca ccgcagcagt tcgttgcgag gttcgcggag aagtacgacc taatccgctt
     5101 tgaagccgcg ctagatcaga atgccgctcg ccagttctct gcctccagcc tgaccgacgc
     5161 ttcgagcgga ggcggcaaga cgaagaggcg cgggcttatg gggagggtat gaaggcgata
     5221 caggcgccat gcgtcgggtt gttcttccga cacttcccgt tctgtgcgcg ttaagaagaa
     5281 gtcggtggta gggccaccgc gcgtggcctt gacctcaatc aagcggtcgg aaccgtcggg
     5341 ttcgaaactc cgaatgtcat agcccgcgcc atcgccatgc acgtcggaag tccactctac
     5401 cttcttcgca aggtccatcc gatcggcggc gactaaacgg gcgatctcgt gttccaagac
     5461 gtgctgttct cccagccgtc caagcaggcg gttggcatta tcgcgcttgg cgggatcgta
     5521 tttccgtacc agccgaacga ggccctcagg gcgaggtttc cggggcgtga ggaacggcgg
     5581 cggatcggtg ggaaggacga tatctgaagc gcggggctcg ggggttagcg tgcccgagta
     5641 gccctcgctt gcctcggcaa agcccgggtt cgagtttgtc gtctctctgc tctgtgggcc
     5701 tcgggcgcgc gtgggggcaa aaatgctttg accgagcatc cacgcattgg ggtgagtatc
     5761 aagataccgt tccagcgccg ggaaaagcgc ccgttgatag tttgccatcg gtcggtatcc
     5821 gcgaatgtgc ggcaacccca gttcagccag cgcagccgac acgttc
//
LOCUS       DASVTM010000037         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_216713,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000037 DASVTM010000000
VERSION     DASVTM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216713"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..640)
                     /locus_tag="VF633_00895"
     CDS             complement(341..640)
                     /locus_tag="VF633_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="HEX8469644.1"
                     /translation="MKTSALLSTLLAFSAIAACAPMEGGSPTPPPSASLPGECKADAY
                     RSYVGKNRSELPATPAGETRRVVCSTCAVTMDFNPDRVNIVYDTTSNLVTEVKCG"
     gene            complement(662..991)
                     /locus_tag="VF633_00900"
     CDS             complement(662..991)
                     /locus_tag="VF633_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469645.1"
                     /translation="MIAGLLSTGLLAGCSTPAAAPTPAPTVVAEAQATQPVGTRMPQT
                     SADMCKANDLQWLVGRNKSEIPVPVDVVNRRVVCTSCAVTEDYSPYRLNIFFNAQTDV
                     VEQVRCG"
     gene            complement(988..1449)
                     /locus_tag="VF633_00905"
     CDS             complement(988..1449)
                     /locus_tag="VF633_00905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX8469646.1"
                     /translation="MTDTTGPEIWFYHLERSTLDQVLPELLGRTLQRGWKALVRVADE
                     RRLEDIDERLWTWREDSFLAHGRADEPNADRQPILLTQSLENPNGAQALFIIDGSDLG
                     ATETYQRCFIIFDGRDDTALTGARERWKDLKGQGANLAYWKQTDEGWQKAA"
     gene            complement(1446..1646)
                     /locus_tag="VF633_00910"
     CDS             complement(1446..1646)
                     /locus_tag="VF633_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469647.1"
                     /translation="MSFSPLETKSLALRDYLFELIEKAGQIEDGLDFQRGYIMGIQHA
                     LESLQNKLESFGLSQAEVLSIK"
     gene            complement(1646..>2806)
                     /locus_tag="VF633_00915"
     CDS             complement(1646..>2806)
                     /locus_tag="VF633_00915"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX8469648.1"
                     /translation="TLDFGHLTPEQSARSGFAVRLAAYTFNKYRTTLKPEKIASIKTV
                     RIVTSDIRASEAAYEPMSAVADAVVFARDLVSEPANVLYPAEFGRRVKELERLGLKVE
                     ILGEAEMEKLNMWTLLGVGMGSRRESQLAVIHWNGGEPGAQPLAFVGKGVCFDTGGIS
                     IKPADGMEDMKWDMGGAAAVAGLMMALAGRKAKVNAVGVLGLVENMPDGNAQRPGDVV
                     MSMSGQSVEVINTDAEGRLVLADALWYTQQRFQPKFMIDLATLTGAAIVAVGLDIAPL
                     YANSEEVAGPILAAAEKVGENFWRMPLPPQYEQHIDSKIADVKNTGNGRAGGSITAAL
                     FLQRFTNGVPWAHLDIAPTAWVNKSPNPTVPEGAVGFGVRTLDRMVADSYEG"
BASE COUNT          477 a          890 c          923 g          516 t
ORIGIN      
        1 ctctacacgc cgcgcacccg ctagggccga tgacccggag cctgacgcgt gtcaggctcc
       61 ggcagtggtc agccgcaatc ggctttctcg atgacgcccg tctgctggtc gaaccggacg
      121 tttaatcgtt ccggtcggta atcgcgcgtc gtggcacagg tggtgcagac gacacggaca
      181 tactcgctgc gttcgaaatc gacggcgcca acatgggatc cgatcagcga ttgtgcagcc
      241 gacgcattac actgccgagg cgcggtcggc cccgccggag cgggcgcggt cgccgcgcac
      301 ccggccatgg cgaatccgac gatcagaccc gccgcgcccc tcatccgcat ttcacttccg
      361 tgaccagatt gctggtggtg tcatagacga tgttcacccg atccggattg aaatccatcg
      421 tcacggcgca ggtcgagcaa accacgcggc gcgtttcgcc agcgggcgtc gctggcagtt
      481 ccgagcggtt cttgccgacg tagctccgat aggcgtccgc cttgcattcg ccgggcagtg
      541 acgcggacgg tggcggcgtg ggggatccac cctccatcgg cgcgcaggcg gcgatggcgg
      601 aaaaggccag cagggtcgag agcagcgctg aggtcttcat ggatgtcgtc tccttaagcg
      661 gtcagccgca gcggacctgt tcgaccacat cggtctgggc gttgaagaag atgttcaagc
      721 ggtagggcga gtagtcttca gtcactgcgc aactggtgca gaccacgcga cggttgacga
      781 cgtcaacggg cacggggatc tcggacttgt tgcgtccgac cagccattgc agatcgttgg
      841 ccttgcacat atccgcgctg gtctggggca tgcgggtgcc gaccggttgg gtcgcctggg
      901 cctcggctac caccgtcggc gcgggtgtcg gagccgccgc cggggtcgaa cagccggcca
      961 gcagaccggt gctcaaaagg ccggcgatca cgccgccttt tgccagcctt cgtccgtttg
     1021 cttccagtag gcgagattgg cgccctgccc cttcagatcc ttccagcgct cccgtgcgcc
     1081 ggtcagcgcc gtgtcgtcgc gtccgtcgaa aatgatgaag catcgctggt aagtctccgt
     1141 ggcgcccaga tctgagccat cgatgatgaa cagcgcctga gcgccattgg ggttctcaag
     1201 cgattgcgtc agcaggatgg gttggcggtc ggcattaggc tcgtcggcgc ggccatgggc
     1261 gaggaagctg tcctcgcgcc aggtccacag ccgctcatcg atatcctcga gccgccgctc
     1321 gtcagcgacc cgcaccagcg ccttccagcc acgctgcaac gtccggccca gaagctccgg
     1381 caacacctga tcgagcgtcg accgctccag atggtagaac cagatttccg ggccggtcgt
     1441 gtcggtcatt ttatcgacag cacctcagcc tgagagagac cgaagctctc caacttgttt
     1501 tggagactct ccaaggcatg ctggatgccc atgatatacc ctcgctggaa atccaacccg
     1561 tcctcgatct gaccagcctt ttcgatcaat tcgaaaagat agtcgcgcaa agccaggctt
     1621 ttggtttcca gcggggagaa ggacatcaac cctcgtagct atccgcgacc atccggtcga
     1681 gggtgcggac gccgaagccg acagcgcctt cggggacggt ggggttcggc gacttgttga
     1741 cccacgcggt cggggcgatg tcgaggtgag cccagggcac gccgttggtg aagcgttgca
     1801 ggaacagggc ggcggtgatc gagccgccgg cccggccgtt gccggtgttc ttcacgtcgg
     1861 cgattttgga gtcgatgtgc tgctcgtact ggggcggcag cggcatgcgc cagaagtttt
     1921 caccgacctt ctcggcggcg gccaggatcg gtccggcgac ctcttcgctg tttgcgtaga
     1981 gcggcgcgat gtcgaggccg acggcgacga tggcggcgcc cgtcagggtg gccagatcga
     2041 tcatgaactt gggctggaag cgctgctggg tgtaccagag ggcgtcggcc aaaacgagac
     2101 ggccttcggc gtcggtattg atcacttcca ccgactggcc ggacatcgac atgaccacgt
     2161 cgcccggacg ttgggcgttg ccgtcaggca tgttctcgac gaggcccagc acgcctaccg
     2221 cgttgacctt ggccttgcga cccgccagcg ccatcatcag gccggccacg gcggccgcgc
     2281 cgcccatgtc ccacttcatg tcttccatgc cgtcggcagg cttgatcgag atgccgccgg
     2341 tgtcgaaaca aacgcccttg ccgacgaagg ccagaggctg ggcgccaggc tcgccgccgt
     2401 tccagtggat gacggccagt tggctctcgc gacggctgcc catgccgacg ccgagcagcg
     2461 tccacatgtt cagcttttcc atctcggctt cgcccaggat ttcaaccttg aggccgaggc
     2521 gttccagttc cttgacccga cggccgaact cggcgggata caggacgttg gcgggctcgg
     2581 agacaaggtc acgggcgaag accacggcgt cggcgacggc cgacatgggc tcataggcgg
     2641 cttcggacgc gcggatgtcg gaggtcacga tccgcaccgt cttgatcgag gcgatcttct
     2701 cgggcttcag cgtcgtgcga tatttgttga aggtgtaggc ggccagacga accgcgaaac
     2761 cggagcgggc ggactgctcc ggcgtcaggt gaccgaagtc cagcgt
//
LOCUS       DASVTM010000038         6994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_535638,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000038 DASVTM010000000
VERSION     DASVTM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535638"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VF633_00920"
     CDS             complement(<1..893)
                     /locus_tag="VF633_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX8469649.1"
                     /translation="MTTMFALLILTLVAAYMAVLFGIAGRSERLGPRKPGGRFGPWAY
                     ALSLAIYCTSWTYYGAVGTASRDGWEYLPIYLGPIIGIVVLFPIWRRIAAAAKRENIG
                     SIADFISSRYGKSQTLGAVVACVAIFGSLPYIALQLQSLSMAWQLVTAGTAVAGSEQL
                     TVPIIAVVLAGFTILFGARRPDLTEHNRGLIQAIAIESIVKLAALVAVAVFAALLWFG
                     SPDPAAVITALGDLAAAPEINARFIAITLLATLAVFCLPRQFHVGFVEAAEPAHIRRA
                     RWVFPVYLLITSVAVVPLLAAG"
     gene            complement(1005..2678)
                     /locus_tag="VF633_00925"
     CDS             complement(1005..2678)
                     /locus_tag="VF633_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8469650.1"
                     /translation="MTDSAMDAPAMDKAALDKKDRLVILASSVGTVIEWYDFYLYGSL
                     AAIITAQFFSGVNETTGYIFALMAFAAGFAVRPFGAVFFGRLGDIWGRKNTFLVTMML
                     MGLSTFVVGLLPSYAAIGIAAPIILVVMRLIQGLALGGEYGGAATYVAEHAPKGKRGF
                     YTSFIQITATAGLVLSLLVILGVRLTVGEEAFTAWGWRIPFLVSILLLGVSLWIRLKL
                     AESPSFKQMKAEGKGSPTPLKDSFLKWPNLKLVIIALVGLTAGQAVIWYTGQFYALFF
                     LERIMKVDATLVYILLAIALLLACPFFIFWGWLSDKVGRKPIILAGCLIAAITYFPLF
                     NALTHAANPALAAATESQPVTIYADPADCNVQFDPVGKTVFNQSCDVAKSYVAKAGIN
                     YTNVAAPAGTVAEVRIGDSVVVPSFRGDVLPAAEFAAQRKTWETGLGAALATAGYPAK
                     ADSDQVNKPMVVLILFILGFYVTMVYGPIAAALVEMFPTNIRYTSMSLPYHIGNGWFG
                     GFLPTTAFAMVAATGNIFYGLWYPIIVALATVVLGFLLVKEGKDVDLNA"
     gene            3098..4147
                     /locus_tag="VF633_00930"
     CDS             3098..4147
                     /locus_tag="VF633_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8469651.1"
                     /translation="MADYVPEGHRIVTHDLGSQSRRLWLSRAAVSAILPGWADPAGAE
                     AHGVWLGEYFRAAEARTASSLDEALGLGPRPLVRGTTDFVLTQAAEESTLEGAMRRTA
                     LAYNRIHGGDYNRVRRMNGLLIYIIDDARFPYSDMPPPAVTLMLECVLIHLHSCFCAL
                     THSDLTPAVVHVATRRAFTNVEDAMGVWPCGARWGRPWFALAYDEGIGCTPVLTAGPR
                     PSAAIVHARLMHLVERREAELSRQSDLPSRVRAVLRSDLVCDQAEAARRLGCSPATLR
                     RQLTAEGLAFRRLRDEVLNERARLELLGLHPIADIAEILGYADSRSFARAFKRWNGVA
                     PNGFRRSPSEVSLHA"
     gene            4253..6499
                     /locus_tag="VF633_00935"
     CDS             4253..6499
                     /locus_tag="VF633_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469652.1"
                     /translation="MTHRSMLAGASAIAMTMGLALQASAQVQSTAPAPVQPEDNVFQV
                     EEIIVTARRQSERLQDVPSAVTAFSADTLYRNQTQTLGDLQAQVPNLSLHVGDASNAV
                     IYLRGIGQIDSISFNDPGVGVYLDDVYLGRVQGSFLDVIDPQQIEVLRGPQGTLYGRN
                     TIGGAVKFTSQKPTATPGGYIAGGVGSYGERRLRARLAGPVGSDQLLGSIAVAASRRD
                     GYTDNLGDGETDGDKNLLAGRATLIYQPNDRFSANLTVDASRNDPRRSRTPHRETPIY
                     SVNTGGIRPVITDPYTVDVTYNDLEKLNTSGVALTLAYDAGPFTFKSISAYREMDYRT
                     HLDLDASPDRSFGIYDFETQDQTSQELQILYHGDRLSAVGGLFYFQENDWTFGGAVAP
                     DFFVDIGFILPFPLNTAGLRLSDNESIAAYGQIDYDLTDRASLTLGLRYTHETKATDN
                     RGENFSPAVASTAEDMERLFGQGFGSGLTGYQADESWDSLTPRVALNYKVSEDVLAYV
                     SAAQGFKSGGFNGRITTSAQSFDPETLWSYEGGLKSELFDGRARVNLAAFFQKYTDLQ
                     LSRFAADPVTGDFTSVFDNAGGADFYGLEAEVSAQLSQALRFDGNLAYLHSEYTEYID
                     GGVDISGQRKLVNAPEWSGRAGLSYDASLGELGDLTLSGGASYRSKTYLTVSSSEVLA
                     QDGYWLTDASILFTPRNAAWTVLLSGKNLTDEAYKEHGFDLSASPGVQLGYYGAPRTV
                     SLDVRYTF"
     gene            6499..>6994
                     /locus_tag="VF633_00940"
     CDS             6499..>6994
                     /locus_tag="VF633_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX8469653.1"
                     /translation="MSDSTLDEEVQALMVRMGADLAGAPADPTVAERRELMTWMSDAY
                     GPPMARVRAAQDRWIKTPVGALRLRIYWPRRLSLSRPGIVVNIHGGGWVLGGPESYER
                     VSRAYCARGDVIVVDVDYRRAPENRFPAAIDDCEWALAWVAENARSLGGDPQRIAVTG
                     DSAGG"
BASE COUNT         1300 a         2228 c         2285 g         1181 t
ORIGIN      
        1 cccgccgcca gcaggggcac gacagcgacc gaggtgatca gcagatacac gggaaagacc
       61 cagcgcgcgc gacggatgtg ggccggctca gcggcttcga cgaagccgac gtggaactgg
      121 cgcggcagac agaagacagc gagcgtcgcc agcagggtga tggcgatgaa gcgggcgttg
      181 atctccggcg ccgcagcgag gtctccaagc gcggtgatga ccgcggcagg atctggcgat
      241 ccgaaccaca gcagcgccgc gaatacggcg accgcaacca gggccgcgag cttgacgatc
      301 gactctatcg cgatggcctg gatcaggccg cgattatgct cggtcaggtc gggacgacgg
      361 gcgccgaaca ggatggtgaa gccggccagc accacggcga tgatagggac ggtcaactgt
      421 tccgaccctg cgacggccgt acccgccgta accagctgcc aggccatcga cagggattgc
      481 agctgcaggg cgatataggg aagggagccg aagatggcga cgcaggcgac aacggcgccg
      541 agcgtctggg acttgccgta tcgcgacgag atgaagtcgg cgatcgagcc gatgttctcg
      601 cgtttggcgg ccgccgcgat acgccgccag atcggaaaca ggacgacgat gccaatgatg
      661 ggccccagat agatgggcag gtattcccac ccgtcccgcg acgccgtgcc gaccgcgccg
      721 taataggtcc atgaggtgca gtagatggcc aggctgagcg cataggccca agggccgaaa
      781 cgaccaccgg gcttgcgcgg acccagccgc tcgctgcgcc cggcgatgcc gaacagcaca
      841 gccatatagg cggccaccag cgtgaggatc agcagggcga acattgtcgt catctagacg
      901 ggctcgggct gggtctcgca agaagtccga gagggtctcg caagaaaaag acgggccgat
      961 ccgaagatcg acccgcctct ggctcgggga ggagcgtctt caggtcaggc gttgaggtct
     1021 acgtccttgc cttccttgac cagcaggaag cccagcacga ccgtcgcgag ggcgacgatg
     1081 atcgggtacc agaggccgta gaagatgttt ccggtcgccg cgaccatggc gaaggcggtg
     1141 gtcggcagga agccgccgaa ccagccgttg ccgatatgat agggcagcga catcgaggtg
     1201 tagcggatgt tggtcgggaa catctcgacc agagcggcgg cgatcgggcc gtaaaccatg
     1261 gtcacgtaga agccgaggat gaacaggatc aggacgacca tgggcttgtt gacctggtcg
     1321 ctgtcagcct tggcggggta tccagccgtg gcaagcgccg cgcccagtcc ggtttcccag
     1381 gtcttgcgtt gcgcggcgaa ctcggcggcc ggcaggacgt cgccccggaa gctcgggacc
     1441 acgacgctgt cgccgatgcg cacctcggcc accgtcccgg ctggggccgc gacattggtg
     1501 tagttgatgc ccgccttggc gacataggat ttggccacgt cgcacgactg gttgaagacg
     1561 gtcttcccga ccgggtcgaa ctggacgtta cagtcggccg gatcggcata gatcgtcacc
     1621 ggttgggact cggtggcggc ggccagcgcc gggttggcgg cgtgggtcag ggcgttgaac
     1681 agcgggaaat aggtgatcgc cgcgatcagg caaccggcca gaatgatcgg cttccgcccc
     1741 accttgtccg acagccagcc ccagaagatg aagaaggggc aggcaagcag cagggcgatg
     1801 gccagcagga tatagaccag ggtagcgtcg accttcatga tccgttcgag gaagaacagg
     1861 gcgtagaact ggcccgtgta ccagatcacg gcctggccgg cggtcaggcc caccagggcg
     1921 atgatcacca gcttcaggtt cggccatttc aggaacgagt ctttcagcgg agtaggggag
     1981 cccttgccct cggccttcat ctgtttgaac gacgggctct cggccagctt caggcggatc
     2041 cacagggaca cgcccagcag caggatcgag acgagaaacg gaattctcca gccccatgcg
     2101 gtgaaggctt cctcaccaac cgtcaggcgg acgcccagga tgacgagcag gctgagcacc
     2161 agtccggcgg tggccgtgat ctggatgaag gaggtgtaga agccgcgttt gcccttgggc
     2221 gcgtgttccg cgacataggt ggcggcgccg ccgtactcac cgcccagggc cagcccctgg
     2281 atcaggcgca tgacgaccag aatgatcggc gccgcgatgc cgatggcggc ataggacggc
     2341 agcaggccga cgacgaaggt cgacaggccc atcagcatca tcgtgaccag aaaggtgttc
     2401 tttcggcccc agatgtcgcc gagacggccg aagaagacgg ccccgaacgg acggaccgca
     2461 aaaccggccg cgaaggccat cagggcgaag atatagccgg tggtctcgtt cacgcccgag
     2521 aagaactggg ccgtgatgat cgcggcgagc gagccgtaca gatagaagtc gtaccactcg
     2581 atcacggtgc cgactgagga cgccagaatg acgagcctgt ctttcttgtc gagcgccgcc
     2641 ttgtccatcg cgggcgcatc catggcgcta tcggtcatcg tatttgcctc ccaaatctcg
     2701 ccgttcatcg gctgttctgt cgccgcgtca ccggcgtcaa ctccaaccgt ggcagcgatt
     2761 ggtgtccagc ggcagatcga ctttggtcga acagaagacg cggcgacctc tacgacggcg
     2821 ccgaaggagc cagaacgcga aggggcctgc ttcaggctcc ggggttggga tcatataggc
     2881 gctctaagcg atggccttgg agaacaggct tcgcgccatg cggttcgcgc gcgctacccg
     2941 ggcagttcgt ctcttgcttg gcaagtattg cccggggacc gtcaacaccg gcggatagaa
     3001 gcctcggctg aaggaagtag gtggctcgtt gaccagacct gttccgcaca gcacgcattc
     3061 tcacgctgct gacaatgagc tttgtggtta gcgtcagttg gccgattatg tccctgaggg
     3121 gcaccgcatc gtgacgcatg atcttggttc gcaatccaga aggttgtggc tgtcgcgtgc
     3181 ggcagtcagc gccatactgc ccgggtgggc tgatccggcc ggcgccgagg cgcacggcgt
     3241 ttggctcgga gaatatttcc gcgccgctga ggcgcgcaca gcctcgtcac tggacgaggc
     3301 gctcggtttg ggaccacggc ccctggtacg cggcacgacg gatttcgtcc tgacccaagc
     3361 cgccgaggag tcgacgctgg agggcgccat gcgccgcacg gccctggcct acaaccgcat
     3421 ccacggggga gactacaacc gggtgcgccg gatgaacggt ctgctgatct acatcatcga
     3481 cgatgcccgt tttccctatt cggatatgcc gccgccagcc gtgaccctga tgctggagtg
     3541 cgtcctgatt catctacaca gctgcttttg cgctctgact cacagcgatc tcacccccgc
     3601 tgtcgtgcat gtagcgaccc gcagggcgtt tactaacgtc gaggacgcca tgggcgtgtg
     3661 gccttgcgga gcgcgatggg gccggccgtg gttcgccctg gcctacgacg aggggatcgg
     3721 ctgcacccct gtcttaaccg ctggtccgcg tccgtcggcg gcgatcgtcc acgcccgcct
     3781 gatgcatctc gttgagcggc gcgaagccga gctgtcccgc cagtccgacc tgccgtcccg
     3841 ggtccgggct gtgcttcgga gtgatctggt ttgcgaccag gccgaggcgg cgcgccgtct
     3901 gggctgcagt ccggcgacct tgaggcgcca actgactgcc gaagggctcg cctttcgtcg
     3961 actgcgtgac gaggtgttga acgagcgcgc ccggctggaa ttactgggtt tgcacccgat
     4021 agccgatatc gccgaaatac tgggctatgc cgatagccgc agcttcgcgc gggcattcaa
     4081 acgctggaac ggcgttgccc ccaatggctt tcgtcgttca cccagcgagg tctcacttca
     4141 cgcctgagcg aatttgtccc ccgccgagcg gcgattcggt cgtcgacgcc cgcccccggg
     4201 actctttagc tgctcgccga acgcgtcaca aaaggcgcga cgggaggatg agatgacaca
     4261 ccggtccatg ctggcgggcg cttcagcgat cgccatgacg atggggctcg ccctgcaagc
     4321 ctccgcccag gtccagagca cggcccccgc acctgtgcag cctgaggaca atgtcttcca
     4381 ggtcgaagag atcatcgtca cggcgcgccg tcagtccgag cggttgcagg acgtgccgtc
     4441 ggctgtcacg gccttctcgg ccgacacctt gtatcgcaat caaacccaga ccctcggcga
     4501 ccttcaggcc caggtgccaa acctgtcgct tcacgtgggc gacgcgtcca atgcagtcat
     4561 ctacctgcgc ggtattggcc agattgactc gatctcgttc aacgatccgg gagtcggcgt
     4621 ctatctggac gacgtctatc tgggcagggt gcagggctcg ttcctcgacg tcatcgaccc
     4681 gcagcagatc gaggttctgc gcggccccca aggcaccctg tatggccgaa acaccatcgg
     4741 gggagcggtc aagttcacca gtcagaagcc gaccgccacg cccggcggct acatagctgg
     4801 cggggttggg agctatggcg agcggcgcct gcgcgctcgt ctggccggac cggtgggctc
     4861 ggaccagttg ctcggctcca tcgcagtcgc cgcctcgcgg cgcgatggct acaccgacaa
     4921 tcttggcgac ggcgaaaccg acggcgacaa gaaccttctc gccggacgcg ccacgctgat
     4981 ctatcagccg aacgatcgct tttcggccaa tctcacggtc gatgcgtccc gaaacgaccc
     5041 gcgccgctcg cgaacaccgc atcgcgaaac gcccatctat tccgtgaaca ccggcggcat
     5101 acgcccggtg atcacggatc cctacacggt cgatgtcacc tacaatgatc tggagaagct
     5161 gaacacgtcg ggcgtggcgt tgacccttgc ttacgacgca ggccccttca ccttcaaatc
     5221 catcagcgcc tatcgcgaga tggactatcg cacccatctg gatctcgacg cctcccctga
     5281 tcggtcgttc ggcatctacg acttcgagac ccaggaccag accagccagg aactccagat
     5341 cctctatcat ggagaccgtc tctcggcggt tggcggcctg ttctactttc aggaaaacga
     5401 ttggacgttc ggcggcgctg tggccccgga cttcttcgtc gacattggct tcatcctgcc
     5461 cttcccgctg aacacagccg ggctgcgctt gtcggacaac gaaagcatcg ccgcctacgg
     5521 tcaaatcgac tacgacctga ccgatcgcgc cagcctgacg ctgggcctgc gctacactca
     5581 cgaaaccaag gccaccgaca accgcggtga gaacttttca ccagcggtcg cctccacggc
     5641 cgaggacatg gagcggctgt tcggccaagg gttcggctct ggcctcaccg gctaccaggc
     5701 ggatgaaagc tgggattcgc tcacgccccg cgtcgctctg aactacaagg tctccgagga
     5761 cgtcctggcg tatgtcagtg cggcccaggg tttcaagagc ggcggcttca acggccggat
     5821 caccacgtcc gcccagagct tcgatcccga gaccctctgg agctatgagg gcggactgaa
     5881 gtccgagctg ttcgacggcc gcgccagggt caacctggcc gcgttcttcc agaaatacac
     5941 cgaccttcaa ctcagccgct tcgccgccga cccggtaaca ggtgatttca cctcggtgtt
     6001 cgacaacgct ggcggcgccg acttctacgg gctcgaggca gaggtctcgg cccagctgtc
     6061 ccaagccctg cgcttcgacg gtaatctggc ttatctgcac agcgagtata ccgaatacat
     6121 cgacggcggc gtggatatca gcggccagcg caaactggtc aatgctccgg aatggagcgg
     6181 tcgggcgggt ctgagctatg acgcctcact gggcgaactg ggcgacctga ccctgtcggg
     6241 cggagcctct tatcgctcca agacctatct gaccgtctcc agcagcgaag tgttggccca
     6301 ggacggctat tggttgaccg acgcctcgat cctgttcacg ccccgaaacg ccgcctggac
     6361 ggtgctgctc agcggcaaga acctgacgga tgaggcttat aaggagcacg gcttcgacct
     6421 cagcgcctcg cccggcgtcc agctcggcta ctacggcgcg ccacgcacgg tcagcctgga
     6481 cgtccggtac acattctgat gtcggactcc acgctggatg aggaggttca ggcactgatg
     6541 gtccgcatgg gggccgacct cgccggcgcg ccggcggatc ccacggtggc ggagcgacgg
     6601 gagttgatga catggatgtc cgatgcctac ggcccgccga tggcgcgggt ccgggcggcg
     6661 caggatcgct ggatcaagac gccggtcggc gccctgcggc tgcgcatcta ctggccgcga
     6721 cggctcagcc tgagccgacc aggcatcgtc gtgaacattc acggcggcgg gtgggtcctg
     6781 ggcggaccgg agtcctacga acgggtcagc cgggcctact gcgcccgggg cgacgtgatc
     6841 gtggtcgacg ttgactatcg gcgcgcgccc gagaaccggt tccccgccgc tatcgatgac
     6901 tgcgaatggg cgttggcatg ggttgccgag aacgcccggt cactgggcgg cgacccccag
     6961 cggatcgccg tcaccggaga cagcgcgggc ggct
//
LOCUS       DASVTM010000039         6355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_322662,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000039 DASVTM010000000
VERSION     DASVTM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322662"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VF633_00945"
     CDS             <1..833
                     /locus_tag="VF633_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX8469654.1"
                     /translation="YILAEGLGRFRFDGPDTIAAFAREHGLRLHGHTLIWYAQGEQAF
                     AGLDHAAFTSAFDRYIATVAGRYRGQVVSWDVVNEPVTDEGSELRDCHWSRMLGQDGY
                     MIRAFHKARAADPDAVLFLNDYNLENTPRKGATFLKLAERLMKAGAPIGGIGTQSHLD
                     IRIPAGQIRDFFRDAASLGLPIHVSELDASLRSEDRVDLRSRAQRIEQQTARVGELAE
                     AFMALPERQRFAFSIWGLRDTDSWLRQGERDDGEDQPLVFDDSGQPNPMYAAVTKAFV
                     "
     gene            868..1779
                     /locus_tag="VF633_00950"
     CDS             868..1779
                     /locus_tag="VF633_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8469655.1"
                     /translation="MTTFVLPGALQRRVEVLANDFMRSDGVPALDFSQPTGEPALIAA
                     DSVSWRIFKNPIALFIGGVAAVILELAEARVRSGVWDHTSFRTDPAPRLKRTGLAAMV
                     TVYGARSRAEAMIAGVNRRHAAVTGRADDGSAYAANDLELLDWVQATASYGFIEAYHR
                     FARPLSPEDRDRAWAEAGPAARLYGATGAPTSRAEWEVQLDAITPKLTASPIVFEFLD
                     IMRAAPLMPTVAKPLQSLLIRAAVDLTPPKVARIVGLEPRHGLSSWQRTIVRAAARTA
                     DRLPLFQSPPAQACVRLGLPADWLYRR"
     gene            complement(1858..2247)
                     /locus_tag="VF633_00955"
     CDS             complement(1858..2247)
                     /locus_tag="VF633_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469656.1"
                     /translation="MKTTMILGAACALAFATAACSGRDDSAQTATDATATDAGSAMAP
                     AAGTTGGTSGAMAPSGSMSTTTNGQTDANGMAGSSTTGSAGTTSGAMGSTTGSTGANG
                     GSTGSTSSGGTSGSTGTSTGSTTPPTH"
     gene            complement(2405..3106)
                     /locus_tag="VF633_00960"
     CDS             complement(2405..3106)
                     /locus_tag="VF633_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8469657.1"
                     /translation="MRVLLIEDDHATAQSIELMLKSEGFNVYTTDLGEEGIDLGKIYD
                     YDLILLDLNLPDMSGLEVLRQLRLGKINTPVMILSGSTEIETKVKTFGGGADDYMTKP
                     FHKDELIARTHAVVRRSKGHAQAIIHTGEIAVNLDGKTVEVHGHRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLATAAGGKHYIETVWGRG
                     YVLRDPSEGIQTVAA"
     gene            3472..3858
                     /locus_tag="VF633_00965"
     CDS             3472..3858
                     /locus_tag="VF633_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HEX8469658.1"
                     /translation="MMLHVQMEETAYRVPVAEGDRTRTGLAIQVIALTFDLPMSAMAG
                     DRLGVKACRARWMAIYLAHVGYGWTLERVSHAFGVNRTTASAACRWSEDARDDAAIDA
                     ALDRMEQTLRLVCEMPRLGLPLGGRG"
     gene            3855..4562
                     /locus_tag="VF633_00970"
     CDS             3855..4562
                     /locus_tag="VF633_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEX8469659.1"
                     /translation="MSAAAERARRMLVRPGAWIDQQDGRYPLRVGGDRRSRISLTLEE
                     ADFRALVSDPGLKVRAGGGWTARQACDVEAPPYGSPGVVPGQRTLMDDEGRLITRAAN
                     LGETPIAWLARRRDASGRPWLTPAEVAAGERLRQDGERAAAGPSVTMRWDGLPRSGGG
                     SATRVEPGDRAISASAHVDAALQAVSPRVRAFVVHICLRSSSLQVAERELGLRRRQGK
                     TMLKQGLQALAEHYGIG"
     gene            complement(4576..5004)
                     /locus_tag="VF633_00975"
     CDS             complement(4576..5004)
                     /locus_tag="VF633_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX8469660.1"
                     /translation="MTEPVSSIADTFAEMVENFDLLEDWEQRIEYVIDLGKDLELLPE
                     ADRVEANKVPGCAAQVWLATDRLNGRLVFHADSDSAISKGNVALLLALFSNRSPAEIL
                     SFDAKAALDRLGLPQALTRQRANGLNSMVGRIRQEALAGA"
     gene            5198..>6355
                     /locus_tag="VF633_00980"
     CDS             5198..>6355
                     /locus_tag="VF633_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX8469661.1"
                     /translation="MNLTPALSTAERAHDLRAVVAWHGVWAVAAGLTAFAAHWLEPGL
                     QLPVLAGLIGMVVPGLAGLILARKDGWIDRALVLGVWSLAAVAASALSGGVTGSMAAF
                     VFMPFVAGILLGGEILSLTGAFAVALAALVGLVSVLLGGAPGDSPALAALVAVVTALA
                     AGIAIRLVWRPREDVLGETRQSLGRVESLLAGQPGMTLVMDAGGRVLGAYGTPPPALE
                     VDPLFTQGLIAQVHAPDRGFVLAALERARTGLQTQVTFAPRAALDRRVTVVLRRLDGA
                     DDTVRLIAQAFDGTPQFARESGLENARAEAEAQNAGKTRFLANMSHELRTPLNAVLGF
                     SDIMRQRLFGPLPDRYADYADSIHEAGGHLLDLINDVLDVSKIEAERYELSL"
BASE COUNT         1032 a         2066 c         2079 g         1178 t
ORIGIN      
        1 aatatatcct ggccgaaggg ctgggcaggt tccgcttcga cggtcccgac acgatagccg
       61 ccttcgcccg ggagcacggc ctgcgtctgc acggccacac gctgatctgg tacgcgcagg
      121 gagaacaggc cttcgccggt ctcgaccacg ccgcgttcac ctcggccttc gatcgctata
      181 tcgccaccgt ggccggtcgc taccggggtc aggtggtcag ctgggacgtc gtcaacgagc
      241 cggtcaccga cgagggctcc gaattgcggg attgccactg gagccggatg ctggggcagg
      301 acgggtacat gatccgcgcg ttccacaagg cgcgcgccgc cgacccggac gccgtcctct
      361 ttctcaacga ctacaatctt gagaacaccc cccgcaaagg cgcgaccttc ctgaagctgg
      421 ccgagcggct gatgaaggcg ggcgcgccca tcggcggcat cggcacccag tcgcacctcg
      481 acatccgcat ccccgctggc cagatccgcg acttcttccg cgacgccgcc tcgctcggtt
      541 tgccgatcca tgtgtcggag ctcgacgcct ccctgcgcag cgaggaccgc gtcgatctgc
      601 gcagtcgggc acagcgcatc gagcagcaga ccgcgcgcgt cggtgaactg gccgaagcct
      661 tcatggccct gccggagcgt cagaggttcg ccttctcgat ctggggcctg cgcgacaccg
      721 acagctggct ccgtcagggc gagcgcgacg acggcgagga tcagccgctg gtgttcgatg
      781 actcagggca gcccaatcct atgtacgccg cggtcaccaa agccttcgtt tgagggaacc
      841 ggcgccacgg ccgagctttc ccaccccatg accaccttcg tcctgcccgg agcgttgcag
      901 cgccgcgtcg aggtcctcgc caacgacttc atgcgctccg acggcgtccc tgcgctcgat
      961 ttctcccagc caacaggcga accggccctg atcgcggcgg actccgtgtc ctggcgtatc
     1021 ttcaagaacc cgattgcgct gttcatcgga ggggtcgcgg cggtcatcct ggagctggcc
     1081 gaggcgcggg tgcgttccgg cgtctgggat cacaccagct ttcgcaccga tcccgccccg
     1141 cgcctgaaac gcaccggttt ggcggccatg gtcaccgtct atggcgcgcg gtcgcgggcc
     1201 gaggcgatga tcgctggcgt gaaccggcgt cacgccgcag taaccggtcg tgccgatgac
     1261 ggcagcgcct atgcggccaa cgatctcgag ctgctggact gggtgcaggc gacagcgagc
     1321 tatggcttta tcgaggccta tcatcgcttc gcccgccctc tgtcgccgga agatcgcgac
     1381 cgggcatggg ccgaggctgg acccgctgca cgcctctacg gggcaaccgg agcgcccacc
     1441 tcgcgggcgg agtgggaggt gcagctggac gccataacgc caaaactaac cgcatcgccg
     1501 atcgtgttcg agttcctcga catcatgcga gcggcgccgc tgatgcctac ggtcgccaaa
     1561 cccctgcaat ccctgttgat ccgggcggcg gtggatctca cgccgcccaa ggtcgcgcgg
     1621 atcgtggggc tagagccgcg ccacggcctg tcgtcatggc agcggacgat cgtgcgggcc
     1681 gctgcacgga cagcggaccg tctgccgctg ttccaatccc cgccggctca ggcctgcgtg
     1741 cggctgggcc tgccggcgga ctggctctac cgtcgctgaa cgaaaacgcc cgccggatgt
     1801 ctccgacggg cgcaggagtc tccgcccctg gacggaatga attgtttgta cggaggacta
     1861 gtgagtgggc ggggtcgtgg aaccggtcga ggtccccgtc gacccgcttg tgccgccgga
     1921 gctcgtggac ccggtggatc caccgttcgc gcccgtggaa ccggtggtgg agcccatcgc
     1981 gccagaggtc gttccggcgg aaccggtggt cgagcttccg gccatgccat tcgcgtcagt
     2041 ctggccattg gtcgtggtgg acatcgaccc gctcggagcc atggcgccgg atgtgccgcc
     2101 agtcgttccc gcggctggag ccatggcgga acccgcatcc gttgcggttg cgtcggtcgc
     2161 cgtctgggcg ctgtcgtcgc gaccgctgca ggccgccgta gcgaatgcga gggcgcaggc
     2221 agcccccagg atcatggtgg ttttcatttt cgttcccttg gatgtcaggt cacgaaatcg
     2281 cgacctcgat tttccaacag ggaaggcccg gaacggttcc taagtcggaa aaccgccaga
     2341 tcagtaacga aaacgcccgc cggcagtgcc ggcgggcgtt tctgatgtct tcgtggtcag
     2401 agattcaggc agcgactgtc tggatgcctt ccgacgggtc acgcagcaca tatcctcggc
     2461 cccagacggt ttcgatgtag tgtttgccgc cagcagccgt agccagcttc ttgcgcagct
     2521 tgcagatgaa cacgtcgatg atcttgagct caggctcgtc cataccgccg tagaggtggt
     2581 tcaggaacat ttccttggtc agcgtcgtac ccttgcggag cgaaagcagc tccagcatct
     2641 ggtattcctt gccggtcagg tggacgcggt gaccgtgcac ttcgaccgtc ttgccatcca
     2701 ggttcacggc gatctcgccg gtgtggatga tggcctgggc gtggcccttg gagcgacgga
     2761 ccacggcgtg ggtgcgcgcg atcagctcgt ccttgtggaa aggcttggtc atgtagtcgt
     2821 cggcgccgcc gccgaacgtc ttgaccttgg tttcgatctc ggtcgaaccc gacaggatca
     2881 tgaccggggt gttgatcttg ccgagacgca gctggcgcag gacttccagg ccgctcatgt
     2941 ccgggaggtt caggtcaagc aggatcaggt cgtagtcata gatcttgccg agatcgatgc
     3001 cttcctcgcc caaatcggtc gtgtagacgt tgaagccctc cgacttcagc atcagctcga
     3061 tgctctgcgc ggtcgcatga tcgtcttcaa taagcagaac gcgcattaat gcccctccag
     3121 gcaaagcttg accatataag ccggtaacca atctcgtggt gtttccgggt aaccttgttc
     3181 atgagttaac cggtgaaaac cttaagaaca gttaacgcca ccccattgag gcgaatcgta
     3241 ggctgatttg cgaatcggcg tcgagcgcca accgttaacc atctccgatt tcttcgttcg
     3301 cgggcccaga ggcctgaaat ggcggctaga attatcgaac ccccacaaca ggggcgagcc
     3361 attctcaaag cgcgcctcgg gatatgaccg gaactgacgt tgaataggac attattccta
     3421 tcgtggctcg ctggcgtttt aggctgcctg caagtttggt cacggagttg gatgatgttg
     3481 cacgtgcaga tggaagagac ggcgtaccgg gtcccggtgg cggagggaga ccggacgcga
     3541 acgggtctgg cgatacaggt catcgccctg acgttcgatt tgccgatgtc cgcaatggcc
     3601 ggcgaccgcc tcggggtgaa agcctgcagg gcgcggtgga tggccatcta cctggctcat
     3661 gtcggttacg gatggacgtt ggagcgtgtc agccacgcct ttggggttaa tcgcaccact
     3721 gcaagcgccg cctgtcgctg gtccgaagac gcgcgcgatg atgcggcgat cgacgctgcg
     3781 cttgaccgta tggaacagac tttgcgcctg gtttgcgaga tgccccggct cggactgccg
     3841 ctcggaggtc ggggatgagc gccgcggccg aacgcgcgcg ccggatgctc gtgcgtccgg
     3901 gcgcatggat cgaccaacag gacggaagat atccgttacg ggtcggcggc gaccggagat
     3961 cgcgcataag cctgacgctg gaggaggccg acttccgcgc tctggtctcc gatccgggcc
     4021 tgaaggtacg agccggcggc ggttggactg ctcgtcaggc gtgcgatgtg gaggctcccc
     4081 cttatggcag ccccggggtg gttccgggcc agcgcacgct tatggacgac gagggccgcc
     4141 tgatcacccg tgcggccaat ctgggcgaaa caccgatcgc atggctggcc cggcggcgcg
     4201 atgcgtccgg ccgaccgtgg ctgacgccgg cggaggtcgc agccggcgaa cggctgcgtc
     4261 aggacggaga gcgagccgcg gccggaccgt ccgtaaccat gcgctgggac ggcctgccgc
     4321 gttccggcgg cgggtcggcc acacgggtcg agcccggaga cagggcgatc tcggcttcgg
     4381 ctcacgtcga tgcggcgtta caggcggtta gcccgcgcgt ccgcgccttc gtcgtgcata
     4441 tctgcctgcg cagttccagc ctgcaagtgg ctgaacgcga gctgggcctc aggcgccggc
     4501 agggcaagac aatgctgaaa caaggtctgc aggccctggc ggaacactac ggcatcggtt
     4561 gagaccccag gccgctcagg cccccgccag cgcttcttgc cggatacgtc cgaccatgga
     4621 gttcaggcca tttgcgcgtt ggcgtgtcag cgcctgtggc agaccaagcc ggtccagcgc
     4681 agctttcgcg tcgaaactca ggatctccgc aggcgagcgg ttcgagaaca gggccagcaa
     4741 cagtgcgaca ttgcccttgg agatcgcgct gtcggaatcg gcgtgaaaca ccaggcgacc
     4801 attcagtcga tcggtcgcca gccacacctg tgcggcgcaa cccggcacct tgttggcctc
     4861 gaccctgtcg gcctcgggca agagttcaag gtccttcccg aggtcgatga cgtactcgat
     4921 ccgctgctcc caatcctcaa gaaggtcgaa attctcgacc atttcggcga aggtgtcagc
     4981 aatgcttgag actggctcgg tcatgcgccg ccagataggc cgccggccca tcgagaacaa
     5041 ccggcccgtt aaggatgacc tcgcccatgc tccccagatc aaacgcttcg gtccaccttc
     5101 ctgagtcgcc tgacgcggga gcgccctcgc gccgactcgg ctcgcagtct agatttgccg
     5161 gtcgggcggt acacgtcccg gacgtttgga atcccgcttg aatctcaccc ccgccctttc
     5221 gacagccgaa cgcgctcacg acctgcgcgc cgtcgtggcg tggcatggag tctgggccgt
     5281 ggcagcggga ctgacggcgt ttgcggccca ctggctggag ccaggccttc agctccccgt
     5341 gctcgcgggc ttgatcggga tggtcgttcc cggcctggcg gggctcattc tggcccgcaa
     5401 ggatggctgg atcgaccggg cgctggtgct cggcgtctgg agcctcgccg cggttgcagc
     5461 ctcggccttg tcaggcgggg tcaccggatc gatggccgca ttcgtcttca tgccttttgt
     5521 ggcgggcatc ctgctcggtg gcgaaatcct gtccctgacc ggagcgttcg ctgtcgccct
     5581 ggctgcattg gttgggctgg tctccgttct gctaggcgga gccccgggtg acagccccgc
     5641 cctcgccgcc cttgtcgccg tggtcacggc cctggctgcc gggattgcta tccgactggt
     5701 ctggcgaccg cgcgaagatg ttctgggcga gacccgtcaa agcctgggac gggtcgaaag
     5761 cctcttggcc gggcagccag gcatgaccct cgtcatggat gccggggggc gggttctggg
     5821 tgcttacggc acgccgccac ctgcccttga ggtcgatccg ctcttcacgc agggtttgat
     5881 cgcccaggtt catgctccgg accggggctt tgtcttagcc gcactcgaac gcgcccgcac
     5941 cggcctccag acccaggtga catttgctcc tcgcgctgcc cttgatcgcc gtgtcacggt
     6001 cgtcttacgt cgtctcgacg gggccgacga tacggtccgt ctgatcgccc aggcattcga
     6061 cgggacaccg cagttcgccc gagagtcggg actggaaaac gcccgcgccg aggccgaagc
     6121 ccagaacgcg ggcaagaccc gtttcctcgc caatatgagc cacgaactgc gcacgccgct
     6181 gaacgccgtc cttggcttct ccgatatcat gcgccagcgg ttgttcggcc ccctgccgga
     6241 ccggtacgcc gactatgcgg acagcatcca cgaggcgggc ggccacctgc tggatctgat
     6301 caacgacgtt cttgacgtct ccaagatcga ggccgagcgg tatgagttgt cactg
//
LOCUS       DASVTM010000040         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_588975,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000040 DASVTM010000000
VERSION     DASVTM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588975"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF633_00985"
     CDS             complement(<1..221)
                     /locus_tag="VF633_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX8469662.1"
                     /translation="MGARDPDFETWVHEHVAALGPLEIKRMFGGAGVYRQGTIFALLD
                     DGVVWLKGDESNITALEAAGSRQFTYSGK"
     gene            complement(221..616)
                     /locus_tag="VF633_00990"
     CDS             complement(221..616)
                     /locus_tag="VF633_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX8469663.1"
                     /translation="MTELQTTARPRRPRPKAVTLTDAAAERVREIMSNADRPYIALRV
                     GVKNGGCAGQEYTFAYAEQIEAMDEVVEDKGVTILIDPKAILFLIGSEIDYETTKLAS
                     KFVFRNPNQTDACGCGESVTIIPVAALEA"
     gene            complement(628..1029)
                     /locus_tag="VF633_00995"
     CDS             complement(628..1029)
                     /locus_tag="VF633_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEX8469664.1"
                     /translation="MTDPTLPETPAATDAFAEAWDEPQKSALSQAELNTLTDDLIEAL
                     KTVYDPEIPVDIYELGLIYKVDVNDDRDVVVEMTLTAPGCPVAGEMPGWVEDAVKKVD
                     GVKSARANLVFDPPWDSSKMSDEAKLALNMF"
     gene            complement(1029..2249)
                     /locus_tag="VF633_01000"
     CDS             complement(1029..2249)
                     /locus_tag="VF633_01000"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX8469665.1"
                     /translation="MKTFDPYAARAQFPILSRTVNGKPLVYLDNAASAQKPRAVIDNL
                     VAQMEGSYANVHRGLHTLSNEATDAFEAAREIVARYLNAETPDNIVWTKGGTEAINLV
                     ANGLGLSIQPGDEIIVSEMEHHSNIVPWHLLRERRGAVLKWIPVLDDGSLDMEAYAAL
                     LGPKTRMVAVTHMSNVLGTINPIAEITRLAHAAGAQVLIDGCQGAVHAAPDVQAIGCD
                     FYVITGHKLYAPTGIGALYGKTEALQALPPYQGGGEMIETVERDRITYAKPPHRFEAG
                     TPPIIEAIGLGAALTWLSAFDRAAIAAHENALYDHAVARLEGLNWLRILGQAEGKGAI
                     LSFAVADAHAHDIAQIMDRYGVAVRAGQHCAEPLAKRMGVTSSTRASFALYNTVEDAD
                     AFVDALIKAREFFV"
     gene            complement(2252..>2953)
                     /gene="sufD"
                     /locus_tag="VF633_01005"
     CDS             complement(2252..>2953)
                     /gene="sufD"
                     /locus_tag="VF633_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX8469666.1"
                     /translation="LLETHEGHGGAYVAHNRLTLDVAETAEVTRIVLVDEPANAISVT
                     EAQVKVAPGGRYRQTIVATGAKLQRLETHVSHGGEGADVRLDGLYAVSDARQTDLTSV
                     VSHVAPNGQTSQLTKGVARDTSRGVFQGKIVVERGADGTDARMGHHALILGERAEIDA
                     KPELIIYADDVQCAHGNTVGNLDDDALFYMEQRGIPADEARALLTQAFLIEVVDRIEH
                     DEAREVVRSWLTERL"
BASE COUNT          459 a          944 c          988 g          562 t
ORIGIN      
        1 tccttgcccg aataggtgaa ctggcgcgaa ccggcggctt ccagagcggt gatattgctc
       61 tcgtcaccct tcagccagac cacgccatcg tccagcaatg cgaagatcgt gccttgccga
      121 tagacgccgg cgccgccgaa catccgcttg atctccagcg ggccgagtgc cgcgacatgc
      181 tcgtggaccc atgtctcgaa gtcgggatcg cgggcgccca tcaggcctcc agcgccgcca
      241 cggggatgat ggtgacgctc tcgccacagc cgcaggcgtc ggtctggttt ggattgcgga
      301 acacgaattt ggacgccagc ttcgtggtct cgtagtcgat ttccgagccg atcaggaaca
      361 ggatcgcctt ggggtcgatc aggatggtga cgcccttgtc ctcgaccacc tcgtccattg
      421 cctcgatctg ttcggcgtag gcgaaggtgt attcctgacc cgcgcagccg ccgttcttca
      481 ccccgacacg cagggcgata tagggcctgt cggcgttcga catgatctcg cgcacgcgct
      541 cggcggcggc gtcggtcagg gtcacggcct tgggacgcgg gcgtctcggg cgtgcggtgg
      601 tctgaagctc ggtcatcgtt ttcgctctca gaacatgttc aaggccagct tggcctcgtc
      661 gctcatcttg gaggaatccc acggcgggtc gaacacgaga ttggcccgcg ccgacttgac
      721 gccatcgacc ttcttgactg catcctcgac ccagccgggc atctcgccgg cgacggggca
      781 gccgggcgcc gtcagcgtca tctcgaccac cacgtcgcgg tcgtcgttga cgtcgacctt
      841 gtagatcagg ccgagctcat aaatatcgac cgggatttcc gggtcataga cggtcttcag
      901 cgcctcgatc aggtcgtcgg tcagggtgtt cagctcagcc tgcgacaggg cgctcttctg
      961 aggctcgtcc caggcttccg cgaaggcgtc ggtggccgct ggtgtttcgg gcagggtcgg
     1021 atcagtcatt acacaaagaa ctcgcgcgcc ttgatcagcg catccacgaa ggcatctgca
     1081 tcctccacgg tgttatatag ggcgaagctg gcgcgcgtgc ttgacgtcac gcccattctt
     1141 ttcgccagcg gctcggcgca gtgctggcca gcgcggacgg cgacgccgta tcggtccatg
     1201 atctgggcga tgtcgtgggc gtgggcgtcg gcgacggcga agctgaggat ggcccccttc
     1261 ccctcagcct gaccgagaat ccgcagccag ttcaggcctt ccagacgcgc gaccgcgtgg
     1321 tcgtagagcg cgttctcgtg cgccgcgatg gccgcacgat cgaacgccga cagccaggtc
     1381 agggccgcac ccagaccgat ggcttcgatg atcggcggcg tgccggcctc gaaccggtgc
     1441 ggaggcttgg cgtatgtgat ccggtcgcgc tcgacggtct cgatcatctc gccgccaccc
     1501 tgatagggcg gcagggcctg aagcgcctcc gtcttgccgt agagggcgcc gatgccggtc
     1561 ggggcgtaga gcttgtggcc ggtgatgacg tagaagtcgc agccgatggc ctgaacgtcc
     1621 ggtgcagcat ggaccgcgcc ctgacagccg tcgatcaaca cctgtgcgcc ggcggcatgc
     1681 gccagccgcg tgatctcggc gatggggttg atggtgccca agacattgga catatgcgtg
     1741 accgcgacca tgcgcgtctt tggacccagc agcgcggcat aggcttccat gtcgagcgag
     1801 ccgtcgtcga gcaccgggat ccacttcagc accgcaccgc gccgctcgcg cagcaaatgc
     1861 cagggcacga tgttggagtg gtgctccatc tccgagacga tgatttcgtc gcccggctgg
     1921 atcgacagac cgagcccgtt ggcgaccaga ttgatcgcct cggtgccacc cttggtccag
     1981 acgatgttgt ccggcgtttc agcgttcaga tagcgcgcta cgatctcgcg cgcggcctcg
     2041 aaggcgtcgg tcgcctcgtt cgacagggtg tggaggccgc gatgtacgtt ggcgtatgag
     2101 ccctccatct gggccaccag attgtcgatc acggcgcggg gcttctgggc cgaggcggcg
     2161 ttgtcgagat agaccagcgg cttgccgttg accgtccgcg acaggatcgg gaactgggcg
     2221 cgggcggcgt aagggtcgaa ggtcttcata gttacaggcg ttccgtcagc catgaccgga
     2281 ccacctcccg tgcttcatcg tgttcgatcc ggtccacgac ctcgatcagg aaagcctgcg
     2341 tcagcagcgc gcgggcctcg tcggccggga tgccgcgctg ctccatgtag aacagggcgt
     2401 cgtcatccag attgccgacg gtgttaccgt gggcgcactg gacgtcgtcg gcgtagatga
     2461 tcagctcggg cttggcgtcg atctcggcgc gctcaccgag gatcagggcg tggtgtccca
     2521 tgcgcgcatc ggtgccgtcg gccccgcgct cgaccacgat tttgccctgg aacacgccgc
     2581 gcgaggtgtc acgcgccacg cccttggtca gttgcgaggt ctggccgttc ggggccacgt
     2641 gggaaacaac gctggtcagg tcggtctggc gcgcatcgct cacggcgtac aggccgtcga
     2701 ggcggacgtc tgcgccctcg ccgccgtgcg aaacgtgggt ctcgagccgc tgcagtttgg
     2761 cgccggtggc gacgatggtc tggcgatatc gtccgcccgg cgcgaccttg acctgggcct
     2821 cggtgacgga gatggcgttc gcaggttcat cgaccaaaac gatgcgggtc acttctgcgg
     2881 tctcggcgac atcgagcgtc agccggttgt gagccacata ggcgccgccg tggccctcgt
     2941 gggtttccag cag
//
LOCUS       DASVTM010000041         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_322781,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000041 DASVTM010000000
VERSION     DASVTM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322781"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VF633_01010"
     CDS             <1..665
                     /locus_tag="VF633_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX8469667.1"
                     /translation="DWHPGVVGIVAGRLRERWRKPVIVIGVDAVNGIGKGSGRSQPGM
                     NLGRAIQSAWNDGLLIAGGGHAMAAGLTVRTDGIDRLRDYLNEKLAGEQVEAEIIDRV
                     EIDALIEPGAATRALFESFEQLAPFGPANPEPLFALAGVQAREPVAMSGGHVRCRLVG
                     ADGTSVKSIAWRCADLPLGKALLAGQGGLSVAGRLKADDWNGRKGVQFEIEDVADPRM
                     AS"
     gene            745..819
                     /locus_tag="VF633_01015"
     tRNA            745..819
                     /locus_tag="VF633_01015"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Glu,seq:ttc)
     gene            1058..1621
                     /locus_tag="VF633_01020"
     CDS             1058..1621
                     /locus_tag="VF633_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX8469668.1"
                     /translation="MSDYEGLEAPDVVRITGRVKWFDPGKGYGFIVPDESGHTHAMDV
                     LLHVSCLRECGHEAAGEGAPITFDCARRAKGWQVVHVHELGQGEMPATPPRRSAYDDL
                     KADSRAMHPLVEVHDSEMESATVKWFNRTKGYGFVVRANDPGDIFIHIETLRRFGLED
                     LVPGETVQVVFADGPKGLVVAEIKPGG"
     gene            1646..2119
                     /locus_tag="VF633_01025"
     CDS             1646..2119
                     /locus_tag="VF633_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX8469669.1"
                     /translation="MAFTRRLFVSGLFLLLTACAPAEKGPVDAGGRALEPLTVVTSTG
                     EHDFMVEIADTEEARQRGLMFRQPLEADGGMLFEFETATEQGFWMKNTPSSLDIVYIA
                     ADGKIVSIAAHATPFSESVLNSNGAAKGVLEVRAGRMDEIGAKAGDTIRHPFFTN"
     gene            2186..2262
                     /locus_tag="VF633_01030"
     tRNA            2186..2262
                     /locus_tag="VF633_01030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2220..2222,aa:Pro,seq:tgg)
     gene            2376..>2614
                     /locus_tag="VF633_01035"
     CDS             2376..>2614
                     /locus_tag="VF633_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEX8469670.1"
                     /translation="MLARIYRPAKTAMQSGKAKSKDWRLEFEPATARTTDPLMGWISS
                     SDMNGQVCLTFDTKEEAVAYAERHGIAFRLQEPQEP"
BASE COUNT          467 a          829 c          840 g          478 t
ORIGIN      
        1 acgactggca tccgggcgtg gtcggaatcg tggcgggccg gctgcgcgaa cgctggagaa
       61 agccggtcat cgtcatcggc gtcgacgccg tcaacggcat cggcaagggc tcaggccgct
      121 cccagccggg catgaacctc ggtcgcgcca ttcagtccgc ctggaacgat ggcctgctga
      181 tcgcgggcgg cggtcacgct atggctgcgg ggctgacggt caggaccgac ggcatcgatc
      241 gtctgcgcga ctatctcaac gagaagctgg cgggtgaaca ggtcgaggcg gagatcatcg
      301 accgggtcga gatcgacgcc ctgatcgagc cgggcgcggc cacacgcgcc ctgttcgaaa
      361 gcttcgagca actggcgccc ttcggtcccg ccaacccgga gccgctgttc gccctcgcgg
      421 gagtgcaggc gcgcgagccc gtggccatga gcggcggcca tgtccgttgt cggctggtcg
      481 gcgccgatgg gacctcggtc aagtccattg cctggcgctg cgccgacctg ccgctcggca
      541 aggccttgct ggccgggcag ggcggcttga gcgtcgctgg tcgtctgaag gcggacgact
      601 ggaatggccg caagggcgtt cagttcgaaa tcgaggacgt cgccgacccc cggatggcgt
      661 cctgaacgca aaccagcctt tacagccccg atttggtgtt gcgccccccg gaatcggcgg
      721 ctatatcgcc cgctctcgcg ctgtggtccc ttcgtctatc ggttaggacg ccaggttttc
      781 aacctggaga gaggggttcg actcccctag ggactgccac gccacgccac gccacgcttc
      841 cgcatccaga agcatccgct cactgaagta atgtcgcgac gccgtcccaa agtgaggtgg
      901 tccgttcgaa tatgtggacc tctacgtggc ttttcaggaa atcctgtgcg gaaaaaagtg
      961 gatgtacgcg tgaaacgtgc gtttttgtct tttctctcgt tccccgaacc gtggtaaacg
     1021 agaaccgcct gttgtctacg ggcgggggga agagacagtg tctgattacg aagggctgga
     1081 ggctccggac gtcgtccgga tcaccggccg ggtgaaatgg ttcgacccgg gcaagggcta
     1141 tggcttcatt gtgccagacg agtccgggca tacccacgca atggatgtgc ttctgcacgt
     1201 tagctgcctg cgcgagtgcg gccatgaggc cgctggcgaa ggcgccccga tcaccttcga
     1261 ctgcgcccgt cgcgccaagg gctggcaggt cgttcacgtc catgaactgg gccaaggcga
     1321 aatgcccgcc acaccgccgc gccgctccgc ttacgacgac ctgaaggcag acagccgcgc
     1381 catgcatccc ctcgtcgagg ttcatgacag cgagatggaa tcagccacgg tcaaatggtt
     1441 caatcgcacc aagggatacg gtttcgtggt gcgcgccaac gatccgggcg atatcttcat
     1501 ccacatcgaa acgctgcgcc gcttcggcct cgaggacctg gtccccgggg aaacggtaca
     1561 ggtagtgttc gccgacggtc ccaagggact ggtggtggct gagatcaaac cgggcggatg
     1621 atccgcccct gatagggcgt tcctgatggc tttcacgcgt cggcttttcg tttccgggct
     1681 gttcctgctc ctgaccgcct gtgcgcccgc cgaaaaaggg ccggttgacg ctggcggtcg
     1741 ggcgcttgaa cccctgaccg tcgtcacctc gacgggcgaa cacgacttca tggtcgaaat
     1801 cgccgatacc gaagaggccc gccagcgtgg cctgatgttc cgccagccgc ttgaggccga
     1861 cgggggcatg ctgttcgagt tcgaaaccgc gaccgagcag ggcttctgga tgaagaacac
     1921 gccgagctcg ctcgacatcg tctacatcgc cgcagacggc aagatcgtct cgattgccgc
     1981 ccacgcgacg ccgttttcgg aatcagtgct caattccaac ggcgccgcca agggcgttct
     2041 ggaggttcgt gccggccgga tggacgagat cggagccaag gccggagaca ccattcggca
     2101 cccgtttttc acaaactgac ggtttattcg cttcaggaga tcttcgcgtt tgcggtgcgt
     2161 cgacgtttgt gtcaaatccc gccgccggag tgtagcgcag cctggtagcg catctggttt
     2221 gggaccagag ggtcggaggt tcgaatcctc tcactccgac catccccttt tcggaccgag
     2281 cccacgacag ccgtcgtttc ggagctccga cgtcttgagc cgggcggctt gggacgcgat
     2341 aagggatcac ccgcttccgc cgagagtctt ttcccatgct ggcccgcatc taccgccccg
     2401 ccaagaccgc gatgcagtcg ggcaaggcaa aatccaaaga ctggcgtctg gagttcgaac
     2461 cggcgacggc gagaaccacc gatccgctga tgggctggat cagctccagc gacatgaacg
     2521 gccaggtctg cctgaccttc gacaccaagg aagaggccgt cgcctacgcc gagcgccatg
     2581 gcatcgcctt ccgcctgcag gaaccgcagg agcc
//
LOCUS       DASVTM010000042         6306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_589121,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000042 DASVTM010000000
VERSION     DASVTM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589121"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            741..980
                     /locus_tag="VF633_01040"
     CDS             741..980
                     /locus_tag="VF633_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469671.1"
                     /translation="MTGSSGSSGSSQNGASGSAGSSGSMGSTGSSYGGQSGSSGQGGG
                     YGAGSGAGSQGGAMTGSAQDGAYGGSATQRAGERG"
     gene            complement(1019..1339)
                     /locus_tag="VF633_01045"
     CDS             complement(1019..1339)
                     /locus_tag="VF633_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469672.1"
                     /translation="MSDMFVVAISLAAALSASALFSDAAPPQRYREATSFTLVIADQN
                     RIERTCQPLFGKPGDGQKTLGCTVGKTVTLPNPCDFPETDTYARMVCHELGHINGWPA
                     THDE"
     gene            2903..4258
                     /locus_tag="VF633_01050"
     CDS             2903..4258
                     /locus_tag="VF633_01050"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8469673.1"
                     /translation="MTRPEQVVIIGGGFGGLEAAKALEHAPVEVTLIDRQNHHCFQPL
                     LYQVATATLSPADVAWPIRAILSRQRNVRVVMAHVTGLDTAGRRVCSNRGEFSYDHLV
                     IATGATHAYFGHEDWARYAPGLKRIEDATEIRRRILTPFELAELALDRQERERLTTFV
                     VVGGGPTGVEMAGAIADMAREALPPDFRHVDPRCAQVLLVEAGPRLLSAFPESLSAYT
                     RLALERRGVQVLLGAPVTEIDEDVVVIGDRHIATGAVVWAAGVAASAATDWLGAERDR
                     AGRVLVQPDLSVPGKAGIFVIGDTASVVSASGKPVPGVAPAAKQMGRYVGTVISARAR
                     GKAAPEPFHYRHQGDLATIGRKAAVVNTNRFILRGLPAWIFWSLAHVYFLIGIRNRIG
                     VGFSCAWEYLTFGRRARLITEPANAPAAEAALKDTGASPAAVPAVGHADVVANPGPIW
                     G"
     gene            4882..5811
                     /locus_tag="VF633_01055"
     CDS             4882..5811
                     /locus_tag="VF633_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX8469674.1"
                     /translation="MYYTDKILQFPVRVERPDPVFARALQQAIGGVEGEIRVCLQYFF
                     QAWGGRGATKYRDLLLNTATEEIAHIEMLATAVALNLESAPLSVKEDISNDVAGGSVL
                     NGMDMRHVLSTGLAALPTDANGVPFDCSHVYATGNTAADMTANVAAEASGRVLALRLY
                     NMTDDPGMKDMLSYLIARDAYHQQQFLAVLEDMGGLAANLPIPNTPSPEEMPNAEFAY
                     TTLGFNLDGSLPPQGRWSEGPSLDGRSEFTSRAMQPLGEEPHLAPPRPDSGAQLAQMA
                     GGVTAPGSMKPRHPNESGGVINAIKHALDGDPA"
     gene            6034..>6306
                     /locus_tag="VF633_01060"
     CDS             6034..>6306
                     /locus_tag="VF633_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8469675.1"
                     /translation="MKSLAQQTSAELGGRGSVFSRQKRDHIELDALLHRLNGLAAREQ
                     PPVLLQIYRLVFPHAFAEESVLWPVIRRVLPDGEQLTLQVETEHQAI"
BASE COUNT         1169 a         1895 c         2062 g         1180 t
ORIGIN      
        1 ccatgatgtt acgaacaggt gacacacctg aaccacgacc gccaaagccg cgtcgatgcc
       61 atgctcgcca acaggatcgc ttccgttctg gcttgagaaa cgggtgggca cgggagcggc
      121 gctctcgcgg cgataacagg atcgcgcgtc gtgtcatcca tgggcttcca caggccgccc
      181 cggcatgccg cgatctgaca gggaagtgaa ggtcgtgaac tcgcccgggt aggtcaggcg
      241 cagggcgttc ggtccaggtc agggatggcg gtgccaccgc agccgaaagg ccacgacgcg
      301 accgctgcga gagcttcaaa acctcgtcgc cggacgggag ccattgggtc cacgcgtgag
      361 catgaccctc gatcctgaag ctcgacgtca aagaacttca taggttaaca ttcgttttaa
      421 catttgattt ctaagggggt ttttcgcgtt tcggggggaa cgatgcatga tgttctctgt
      481 tcgaatcctg ggacggatgt cctcagcaaa agggatcgaa tatgaaacgc gtgatcgcca
      541 tctgcggcgc ggccggcatt atcgccgctg ccaccgtcgc ctattctcaa tctagcggga
      601 gcacgtccgg atcgtcgggg cagggctcga caagttcgca gagcagcgga tcgatgaccg
      661 gctcccagtc gggcgcgaac ggtcagtcga tgggctccag tacttcgggt cagtcaggct
      721 caagctccca aggcggtgct atgaccggct cctccggaag ctctggctcg agccagaacg
      781 gcgcgtccgg atcggccggt tcatcgggct ccatgggctc gacgggctcc tcatacggcg
      841 gacagagtgg ctcgtccggc caagggggcg gctacggcgc gggctcgggc gccgggtctc
      901 agggtggggc catgacaggc tcggcccagg acggcgccta cggtggttcg gcgacgcagc
      961 gtgctggcga acgaggctaa cgacggccta tctgatggag ccgcttggcg cggctccatc
     1021 actcgtcgtg ggtggccggc cagccgttga tgtgtcccag ttcgtgacac accatacggg
     1081 cgtaggtgtc ggtctcggga aaatcgcagg ggttgggcag ggtgacggtc ttgccgaccg
     1141 tacagcccag tgtcttctgg ccatcacccg gcttgccgaa caggggctga cacgttcgct
     1201 cgatgcggtt ttgatcggcg atgaccaagg taaaggatgt ggcctcccgg tagcgttgcg
     1261 gaggcgctgc gtcggagaac agcgccgagg cgctgagcgc ggcggcgagg ctgatcgcca
     1321 cgacgaacat atctgacatg atgtttggaa actggagcca ttgagcgccg gacagtccgg
     1381 ctcagagaag gaagccctat catttgttga ggtcaggatg acgcccgagg ggaggctgcg
     1441 gcgctcaaat ctgtgtgacc tgatcgcgtc gcattccgcc tgatcaggcc ggccaccatc
     1501 gcctggtgca aggccagcga gtcgaggaga tgcatccaga gcttcgcgga tattgaaacg
     1561 ccgggctgcg cgataaggaa agggttttga tgaggcgggg atcattgttt gaccctgaag
     1621 tcgatcaaac ttcaacaaca gaaagaagtt atattcacct aaagccggct atgttggaat
     1681 atatcaggcc catactagtt ttgtagttta caatactcgc ctcgaccaag gaacgtttct
     1741 gtatacgtcc attaacacct tcgggtgatt ggaaagcgat ggttcggtac agatggccga
     1801 ttttagagtt cggggcgtcg cctcgcgcgg taaatcggtc gaggcttccg ccgaaatccc
     1861 ctgggatgag acctatggac ccgatctggt cgacgccgtt ctggacatgc ttttggacgg
     1921 tgttaggggg ccgctgagct tccagtccgc cgatagagtg tcagttgtgg agtttacacg
     1981 ccgcctcgca gaaacagcgt attgcgatcc gaccctggcg cgcgagaccg cgctgccaag
     2041 actgcgcgcc gtgcgggata acgtgaaatc tcaaatacga tcaaccttct gcctccagcg
     2101 gaaaccacca tcgagcgctt cgttcatgag tggcgctggg ctcggcacaa ggggcttgag
     2161 gatgccccgg agccccgccg gcaaggcgtc gcttgaaaac gcttccgagg ggcgaacgcc
     2221 gtttggcgct ttggattgtc agaggcgtga tcccgcgcgt ccagcgcccg tacgagcgcg
     2281 ccggccgcag gcgccaatcg gctttgtgcg ccaggtccag tacgttcgct cgaaacgctt
     2341 ccaagctgct caccctggtg actgcagccg aaccggtcgc ggggttctcg cggcaggcaa
     2401 tcgtccttgc gcgctgtcaa accgaggggc gaacttgccg acggcgatgt cagtagatgg
     2461 cgaccagtcg gaggatttct ttgctaaatt ctaaccaacg ttaaggaatg cgccgtcgcg
     2521 acgtgaccct ctggatctca acggggtgta gggcaccggc gggaggtggg gcatggcgcg
     2581 gattttttgt tggatcgggg agttcgccga actcaagctg ataaatccgg ccatcggtgt
     2641 gacgccgaaa atgcgtgcaa gaccgccgag gccactctcc aggaccggac gctgacgatg
     2701 ccggagcggc cggtgtgcca tcgatttatc agccgggatc gccgttgaca cccggttcag
     2761 cgttgcgggt tgggtccccc tccttatggt cgaataggcc ttggatgcct gtgtacctac
     2821 gccgtctgtc ttcgcttttt gcgttggccc ggaacctgcc gaacctgcag cggttgcccc
     2881 ggaacgcgac gcgtagagcg caatgaccag acctgagcag gtcgtgatca tcggaggtgg
     2941 ctttggcggc ctcgaggcgg ccaaggcgct tgagcatgcc ccggttgaag tcaccctcat
     3001 agatcgccag aaccaccact gtttccagcc ccttttgtat caagtggcga cggccaccct
     3061 cagcccggcg gatgtggcct ggccgatccg ggcgatcctg tcccgacaac gcaatgtgcg
     3121 tgtcgtgatg gcgcacgtga ccggactcga taccgccggt cggcgggtat gctccaaccg
     3181 cggcgagttt tcctacgacc acctggtcat cgccaccggc gccacgcacg cctattttgg
     3241 ccatgaagac tgggcgcggt acgcacccgg gttgaagcgg atcgaagacg caaccgagat
     3301 caggcggcgt attctgacac ccttcgagtt ggccgaattg gctttggaca ggcaggagcg
     3361 cgagcgcctg accaccttcg tcgtcgtcgg aggaggcccc accggggtcg aaatggcggg
     3421 cgccatcgcg gacatggccc gagaggcgct gccgccggac ttccgtcacg tggacccgcg
     3481 ttgcgcccag gtgctgctgg tcgaggcagg tccgaggctg ctgtcggcgt tccccgaatc
     3541 cttgagcgcc tatacccggc tggcgctgga gcgacgcggc gtgcaggtgc tgctcggcgc
     3601 cccggtgacc gagatcgacg aggatgtcgt tgtgatcggc gatcgacaca tcgccaccgg
     3661 tgcggtcgtc tgggccgcag gggtggctgc gtcggcagcc accgactggc tcggcgccga
     3721 gagagacagg gcggggcgcg tgctggtcca gcccgacctc tcagtaccgg ggaaggccgg
     3781 aatcttcgtg atcggcgaca ccgcgtccgt cgtttccgcg tcgggcaagc ccgttcccgg
     3841 cgtggcgcct gcggccaagc agatggggcg gtacgtcgga acggtgatct cggcccgcgc
     3901 gagggggaag gctgcgcctg aaccctttca ctaccggcac cagggagacc tggccactat
     3961 cggacgcaag gccgccgtcg tgaacacgaa ccggttcatt ctcagaggtt tgccggcctg
     4021 gatattctgg agccttgccc atgtctactt cctgatcggg atccgcaacc ggatcggggt
     4081 gggatttagc tgcgcctggg agtacctgac cttcgggcga cgcgctcgtc tgatcaccga
     4141 gcccgccaac gcccctgcgg ctgaagccgc tctcaaggac acgggcgctt cgccggcggc
     4201 cgtccccgcc gtgggtcatg cagacgtcgt cgctaacccc ggtccaatat ggggctgatc
     4261 ggcccccggg caggaaacgg cacaggcggt gtcgccggca acctggggcg gaagggtgga
     4321 gagctcgaca aagtgagcct tcttgtcgtc ccggctcgac aggcacagga atttgtagac
     4381 ttggccgttg cgtgtctcga tctccatgat ggagccggcg tcgaagcggc tgcacgccag
     4441 ggcaagtcgg cgggccgcct tctcggccga cgaccctgtt cgaaagatca gaggcgctgt
     4501 gatcccgtcc gcttccacaa cccaccactc cgctgagggt ttgacccgaa cagacttcat
     4561 tgcgacgcca ttctcactct tcctccgtct gtcttaggca ggcgccgggg aggcgtcctt
     4621 gctgtaggat agtcgcacag cgatagatcc aaatagcaac gctacgaggt cagggcggtc
     4681 tggtggggcg atttactgcg acgcttcatg cagaagatgg tggagtgggc cgtccggact
     4741 gggcataagg ttttggcatc ttggacataa ctgaacagca acaacaaagg atagtattcc
     4801 aaacattaat atgtcggaac gatagaccgg catgtggctt gagatgtgat ccttcaaacc
     4861 gaaaagagtt gatcgcaagt catgtactac accgacaaaa tcctccaatt tccggtccgt
     4921 gtcgaacggc ctgatcccgt gttcgcccgc gccctccagc aggcgatcgg cggcgtcgag
     4981 ggcgagattc gcgtctgcct tcagtatttt tttcaggctt ggggcggtcg gggcgcgacc
     5041 aaatatcgtg acctgctgtt gaacacggcg accgaggaaa tcgcgcacat cgagatgctc
     5101 gccaccgctg tggccctgaa cctcgaaagc gccccgctct cggtcaagga agacatctcg
     5161 aatgacgtcg ccggcggctc ggtactgaac gggatggaca tgcgccatgt gctctcgacc
     5221 ggtcttgcgg ccctgccgac ggacgccaac ggcgtgccct tcgactgcag ccacgtctac
     5281 gccacgggca atacggctgc ggatatgacg gccaatgtcg cggctgaggc gtccgggcgc
     5341 gttctggctc tgcggctcta caacatgacc gacgatcccg gaatgaagga catgctgtcg
     5401 tacctgatcg cccgggacgc ctatcaccag cagcagttcc tggcggtgct cgaggacatg
     5461 gggggactgg ctgcgaacct gcccataccc aacacgccgt cgcccgagga gatgccgaac
     5521 gcggagttcg cctacacgac gctcgggttc aatctcgacg gctcgcttcc gccccaggga
     5581 cggtggtcgg aaggaccctc tctggacggc cgctcggagt tcacctcccg cgccatgcag
     5641 cccctcggag aggagccgca cctggcgccg ccccgccctg actccggggc ccaactcgcc
     5701 cagatggccg gcggcgtcac cgcacccggc tcgatgaagc cccgtcaccc gaatgagagc
     5761 ggtggcgtca tcaacgcgat caagcacgcg ctggacggcg atccggcctg acagcatcgc
     5821 cagacgtgac cccgtagcca ttttgcggaa gggctacggg cttcgcgtta gcggatcggc
     5881 gccttgaggt ggggctgcca tcgtcttttg attgggacgg tggcgcaggt gctgccaggt
     5941 aaaccgccga tgagaagaga cttgccaagg ggcacgtacc gggttcgcga tccggcagga
     6001 tcgaaggccg ttggacatat cggaggagac gtcatgaagt cgctagcgca gcagacctca
     6061 gccgaattgg gcgggcgcgg gagcgtgttc agccgccaga agcgtgacca catcgaactg
     6121 gacgcgctgt tgcaccgact gaacgggctt gcagcaaggg agcagccacc ggtgcttctt
     6181 cagatctacc ggttggtgtt tccgcacgcc ttcgctgaag agtccgttct gtggccggtc
     6241 atacgcaggg ttttgccgga tggcgaacaa ctcacgctgc aggtggaaac tgagcatcag
     6301 gccatc
//
LOCUS       DASVTM010000043         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_376904,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000043 DASVTM010000000
VERSION     DASVTM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376904"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VF633_01065"
     CDS             <1..417
                     /locus_tag="VF633_01065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8469676.1"
                     /translation="ILRHETEAHDPTRIVINGPRVALGPAAALSLGMIFHELATNAAK
                     YGALSNAEQASEAMANGRGRVFVDWSVANQAERRLTIHWRELDGPPVAEPTRRGFGSR
                     LIERNVRHDLAGEVKFTYAIDGLRADISIPLDKEND"
     gene            417..794
                     /locus_tag="VF633_01070"
     CDS             417..794
                     /locus_tag="VF633_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8469677.1"
                     /translation="MTLPLEGKTILVVEDESLVAMLLETILEDMGCATLGPIATIDEA
                     LAVIADGQAFDGALLDVNVAGKEIFPVAAALKERGIPFVFSTGYGEGGLPPEWRGQRT
                     IQKPFTEDAVQQALIHALGVMAG"
     gene            complement(852..2084)
                     /gene="hutI"
                     /locus_tag="VF633_01075"
     CDS             complement(852..2084)
                     /gene="hutI"
                     /locus_tag="VF633_01075"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX8469678.1"
                     /translation="MIEPLKADLLLVDCQVAAMTEHGAAYGAIEDAAILIRDGAIVWV
                     GSRADLPDHEATQTDRLDGRWVTPGLIDCHTHLVFGGDRSGEFEQRLEGATYEDIARS
                     GGGIVSSVKATRAASEDELYASALTRLAGLKATGVTTVEIKSGYGLNRDSELKMLRVA
                     RRIGREAGVRVRTSYLGLHAVPPEFRGTDADRAAYVDLAIDDILPAANAEGLVDAVDA
                     YCEPIAFSGDEVSRLFEKARSLGLPVRLHADQLSDGGGAALAARHNALSADHIEHASA
                     AGIAAMGEAGVVAVLLPGAYLMLRETTAPPIALLRAHGVAMAVATDCNPGTSPVASMT
                     AAITLACVQFRLTPEEALAGATRHAAKALGLSDQIGTVEVSKRADLAVWDIVRPGELA
                     YWLGKPLLAKRYVNGQLA"
     gene            complement(2081..2503)
                     /locus_tag="VF633_01080"
     CDS             complement(2081..2503)
                     /locus_tag="VF633_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEX8469679.1"
                     /translation="MLGQRERAPRTGEEQDLAGAGLLLELECLHLSDEAPFALEHRLI
                     ALAAAPEAATTDFADTPPGAWLLDSAQWTEAEHRISAVAASASEARLLGLKSGTACLR
                     VERRTWRDGQGVTRVWQTFPGDRYDLVARFSPARGDRP"
     gene            complement(2513..2800)
                     /locus_tag="VF633_01085"
     CDS             complement(2513..2800)
                     /locus_tag="VF633_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8469680.1"
                     /translation="MSEPATLHHRIYTELEGRILSGDWAPGSRIPFEHELTAQYDCSR
                     MTVNKAITELAGRGLVVRRRRAGTFVAQPRAHAAILAIPDLQAEVAERGQA"
     gene            complement(2797..>3220)
                     /locus_tag="VF633_01090"
     CDS             complement(2797..>3220)
                     /locus_tag="VF633_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX8469681.1"
                     /translation="GRFGIGTDSNVQIGLAEELRMLEYSQRLSLRKRAVMADGRRSTG
                     RALFDRALSGGAQATGSQSGIAVGLPADIVALDTRRIAFEGRTGDAVLDSWIFGGDGS
                     AIHSVWRRGRQVVVEGRHIAREAIEARYRKALATVLVG"
BASE COUNT          546 a         1144 c         1030 g          500 t
ORIGIN      
        1 atcctgcgtc acgagaccga ggcgcatgac ccgacccgta ttgtcatcaa tggtcctcgg
       61 gtggcccttg gtcccgcggc ggccctgtcg ctcgggatga tcttccatga actggccacc
      121 aacgccgcca aatacggcgc gctgtccaat gcggaacaag ccagcgaggc catggcgaac
      181 ggtcgtggcc gggtgttcgt cgactggtcc gtagccaacc aggcggagcg ccgcctgact
      241 atccattggc gcgaactcga tggcccgcct gtcgccgaac cgacccgtcg cggcttcggt
      301 agtcgcctca tcgaacgtaa tgtccgacat gatctggcgg gtgaggtcaa attcacctac
      361 gcaatcgacg gcttacgcgc ggacatatcg attccgctcg acaaggaaaa cgactgatga
      421 cgctcccact cgaaggcaag accattctgg ttgtggagga cgagtccctg gtggccatgc
      481 tgctggagac aatcctggag gacatgggct gcgcaacgct aggccccatc gcgaccatcg
      541 acgaggcgct ggcggtcatc gccgacggtc aggcgtttga cggcgccctg ctggatgtca
      601 acgtggcagg caaggagatc ttccccgtcg cggcggcgct caaggaacgg ggcatcccct
      661 tcgtcttctc gacgggttat ggcgaaggcg gcctgccccc tgagtggcgt ggccagagga
      721 ccatacaaaa gcccttcacc gaagatgcgg tgcagcaggc cctgatccac gcgctcgggg
      781 tcatggcagg ctagggtcag gggcggtccg acgggtcccc gacgagatga tgtcgtcact
      841 ggtggtcggg attatgccaa ttggccgttg acgtaccgct tcgccagcag cggcttgccc
      901 agccagtagg ccagctcgcc cggacggacg atgtcccaaa cggccagatc agcccgcttg
      961 ctgacctcca ccgtgccgat ctggtccgac aggcccagcg ccttggcggc gtggcgggtc
     1021 gcgcccgcca gcgcctcctc gggggtcaac cggaactgaa cgcaggccag agtgatcgcc
     1081 gccgtcatcg acgcgacggg cgacgtgccg ggattgcagt cggtggccac cgccatggcc
     1141 acgccatgag ctcgcaacag ggcgatgggc ggcgcggtag tctcgcgcag catcagatag
     1201 gctccgggca atagcaccgc gaccacgccc gcctcgccca tagccgcgat cccggccgcg
     1261 ctcgcgtgtt cgatatggtc ggcggacagg gcgttgtgac gcgccgccag agccgccccg
     1321 ccgccgtccg acagttgatc ggcgtgcagc ctgaccggca ggcccagcga ccgcgccttt
     1381 tcgaacaacc gggacacctc gtcgcccgag aaagcgatcg gctcgcagta ggcgtcgacc
     1441 gcatcgacca gcccctcagc atttgcggcg ggcaggatgt cgtcgatggc cagatcgaca
     1501 taggcggcgc gatcggcgtc tgtcccccgg aactccggcg gcaccgcgtg cagtccgaga
     1561 taagaggttc gcacccgcac ccccgcctcg cggccgatcc gacgcgccac gcgcagcatc
     1621 ttcagttcgc tgtcacggtt gaggccataa cccgacttga tctcgactgt cgtgacgccg
     1681 gtcgctttca gccccgccag ccgcgtcaga gccgaagcat acaactcgtc ctcagacgcc
     1741 gcgcgggtcg cctttaccga agagacgatg ccgccaccgg agcgggcaat atcctcgtag
     1801 gtcgcaccct ccagacgctg ttcgaactcg cccgaccggt cgccgccgaa gaccagatgc
     1861 gtatggcaat cgatcaaccc gggtgtgacc caccggccgt ccagcctgtc cgtctgcgtc
     1921 gcctcatggt caggcagatc ggcgcgcgac ccgacccaga cgatggctcc gtcgcggatc
     1981 aggatcgccg catcttcgat cgcgccatag gccgcgccat gttccgtcat ggcggcgacc
     2041 tggcagtcga ctagaagcag gtcggccttc aatggctcga tcatgggcgg tctccgcgcg
     2101 cgggcgagaa gcgggccacc agatcatagc ggtcgccggg gaaggtctgc cagacccgcg
     2161 tcaccccctg cccgtcccgc caggtccgtc gctccacccg cagacaggcc gtgccggact
     2221 tcagacccag caaccgggcc tcgctcgcgc tggctgcgac agccgagata cggtgctcgg
     2281 cctcggtcca ctgcgcggag tcgagcagcc acgcgcctgg cggggtgtcg gcgaagtcgg
     2341 tcgtggccgc ctcaggcgca gcggccagcg cgatcagcct gtgctcaagc gcgaacggcg
     2401 cctcgtcgct gagatgcaga cactccagct ccagcaacag acccgctccg gccagatcct
     2461 gttcctctcc ggtgcgcggc gcgcgctcgc gctgacccag caaccggacg ccctaggcct
     2521 gccctcgctc ggcgacttcc gcttgcagat cgggaatggc gaggatggcg gcatgggcgc
     2581 gcggctgggc cacgaacgta cccgcccggc gacggcgcac caccagcccg cgcccggcca
     2641 gttcggtgat cgccttgttc accgtcatcc gcgaacagtc atactgcgcc gtcagctcat
     2701 gctcgaacgg gatgcgcgac ccgggagccc agtcgcccga caggatgcga ccttccagct
     2761 cggtatagat gcggtgatgc agggtggcgg gctcgctcat cctacgagca ccgtggccaa
     2821 cgccttgcga taacgagcct cgatagcttc gcgagcgatg tggcggccct caaccacgac
     2881 ctgcctgccg cgccgccaga cggaatggat cgccgacccg tccccaccga agatccagct
     2941 gtcgagcacg gcgtcgcctg tccgcccctc gaaggcaatc ctgcgcgtgt cgagcgcaac
     3001 gatgtcggcg ggcagcccaa cggcgatgcc ggattgcgat cccgtggctt gcgccccgcc
     3061 ggacagtgcc cggtcgaaca gggcgcgacc ggtcgagcgg cgcccatccg ccatgaccgc
     3121 ccgcttccgc agcgacagcc gctgactgta ctccagcatc ctcagttctt cggccagacc
     3181 gatctggacg ttcgagtcgg tgccgatccc gaaccgtccg
//
LOCUS       DASVTM010000044         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_323160,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000044 DASVTM010000000
VERSION     DASVTM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323160"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VF633_01095"
     CDS             <1..787
                     /locus_tag="VF633_01095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8469682.1"
                     /translation="GAILNRELTSVSDAIAKARESQAAYGETLAEASVDIDNAAQPSD
                     LKQVVTGLSDATSRVQIENATLERRLETSTKEVARLREHLEQVRRDAMTDALTNLANR
                     KAFDEELLRSCEEAERNQTVVTLAVLDIDHFKRFNDTWGHQTGDQVLRYVASVIGRIS
                     KNPRVAARYGGEEFAIIFPGEGALQVETALNAIREEIGSRALRRRSTNDELGAVTISG
                     GLAQRRPGESGSLLLDRADEALYASKHGGRNMVTSAERLSAAA"
     gene            830..1606
                     /locus_tag="VF633_01100"
     CDS             830..1606
                     /locus_tag="VF633_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8469683.1"
                     /translation="MRSFLFNIAYWILSIGYGLTAAFAALTPGREATTWVIRRYVKRM
                     VQAMRVFAGITLEVRGRERLPAGSFIIASKHSSWGDGFATYDQFDDLAFVTGDHLEKF
                     PLLGAVLKKLGAIVVNSCGGREARDSLKQRGADAHAEGRKILIYPEGHLAKVGEKYRY
                     RSGVWHMYRDFDMPVVPLASNLGLFWPQKEYEKHPGTATLEFLEPIPTGLGKDEFLAR
                     LEAAVEGRTAELVAEATGKPVAPAVQVLTEDELKKVKTKI"
BASE COUNT          329 a          569 c          576 g          294 t
ORIGIN      
        1 cggagccata ttgaaccggg aactgaccag cgtttccgac gccatcgcca aggcgcgcga
       61 gtcacaggcc gcctatggcg agaccctggc cgaagccagc gtcgacatcg acaacgccgc
      121 ccaaccttcc gacctcaagc aagtcgttac cggcctgagc gacgccacga gccgggttca
      181 gatcgagaac gcgacgctgg agcgtcgtct ggagacttca accaaggagg tcgcccgtct
      241 tcgcgaacac ctcgagcagg tgcgccgcga cgccatgacc gacgccctga ccaacctcgc
      301 caaccgcaag gccttcgacg aagaactgct gcggtcctgc gaggaggctg agcggaacca
      361 gaccgtcgta acgctggccg ttctggatat cgaccacttc aaacgcttca acgacacctg
      421 gggtcaccag accggcgatc aggtcctgcg ctacgtggcc agcgtcatcg gccgcatctc
      481 caagaaccca cgcgtcgctg ctcgttacgg tggcgaagag tttgcgatca tcttcccggg
      541 tgaaggcgcg ctgcaggtcg agaccgcgct gaacgccatc cgcgaggaga tcggctcgcg
      601 cgcgctgcgc cgtcgctcga ccaacgacga actcggagcg gtgaccattt cgggaggctt
      661 ggcccagcgg cgtcccggcg agagcggaag cctgctgctg gatcgtgccg acgaagccct
      721 ctacgcctca aagcatggcg gacggaacat ggtcaccagc gcggaacggc tctccgccgc
      781 cgcctgatcg ggcgcgagac gtttccgcga accgtgccat agtcatcgaa tgcgcagctt
      841 cctcttcaat atcgcctact ggatcctgtc gataggctac gggctgacgg cggctttcgc
      901 ggcgctgacg cccggccgcg aggcgacgac ctgggtgatc cggcgatatg tgaagcggat
      961 ggttcaggcc atgcgggtgt tcgccggcat cacgctggag gtgcgcggac gtgagcgcct
     1021 gccggccggc tcgttcatca tcgcctccaa gcactccagt tggggcgatg ggttcgccac
     1081 ctacgaccag ttcgacgacc tcgccttcgt caccggcgac catctggaga agtttccgct
     1141 actgggcgct gtgctgaaga agctgggggc catcgtagtc aacagctgcg gcggtcggga
     1201 agcccgggat tcgctcaagc aacgcggagc cgacgcccat gccgaaggcc gcaagatcct
     1261 gatctatccg gaggggcatc tggccaaggt cggcgagaaa tatcgctatc gatccggcgt
     1321 ctggcacatg taccgcgact tcgacatgcc ggtcgtgccg ctggcgtcca acctcggcct
     1381 gttctggccg cagaaggagt acgagaagca cccgggcacg gcgacgctgg agttcctgga
     1441 gccgatcccg acgggtttag gcaaggacga gtttctggcg cggctggagg ccgcggtgga
     1501 ggggcggacg gcggagttgg tcgcggaggc tacggggaaa ccggttgcgc ccgccgttca
     1561 ggtgctgacc gaggatgagc taaagaaggt gaaaacgaag atatgatcct tctcccgtaa
     1621 ggggagaagg gctttacgcc tttctcctcg gcttcttcac cgcagctttc cttagcgcga
     1681 cctcgaacgc gccgcgcgcc cagtcggcgg cctcatcagg gtcatcggtg gcgctgtcgg
     1741 gcaggctcca gtagccgagc ttcatcac
//
LOCUS       DASVTM010000045         5344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_749848,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000045 DASVTM010000000
VERSION     DASVTM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749848"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1021)
                     /locus_tag="VF633_01105"
     CDS             complement(236..1021)
                     /locus_tag="VF633_01105"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8469684.1"
                     /translation="MNVSQSDIAATESLLAFWRDAGVDVCYEDAAVDRTYVPPPALKA
                     VAKATASVVVQNDGRDAISEARRLAAGADTLEALAAAVATCEACDLRGMGARQSVFGR
                     GATDAPILIIGEGPGADEDAQGQPFVGKAGQLLDRMLEAAGLTGKVFITNTVFWRPPG
                     NRTPTPQEQAVCAPFVERAFAILKPKVVLLLGAAAAKSVLQTDEGIMRMRGQWREWRL
                     AEGDVSAPALPTLHPAFLLRQPQAKKQAWADMLSLAARLDEIG"
     gene            1227..2876
                     /locus_tag="VF633_01110"
     CDS             1227..2876
                     /locus_tag="VF633_01110"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX8469685.1"
                     /translation="MEYDVVIVGGGPAGLSAAIRLKQRAAAAGTEINVAVLEKSAEIG
                     GHILSGAVIDPKALNELFPDWKERGAPLETPVTKDRFMILGPMGQFSLPMALMPPMMH
                     NHGCYIASLGNLSRWLAEQAEALGVEVYPGMAASHVVYEEPSGRVKGVVAGVFGIDKH
                     GQPTGEFQPGIELHGKYVFIAEGVRGSLAKTIIARHQLAKDSSPQKFGIGLKELWQVP
                     PEKHQPGLAQHSTGWPLDEFTGGGSFMYHFGDNYVAIGYVVHLSYKDPFLSPFDEFQR
                     FKHHPAIAEHLEGGTRISYGARAITEGGFQSVPKLAFPGGALIGCSAGFVNVPRIKGS
                     HNAMKTGMMAADAAYDAVMAGRSGDELVEYQAAYEKSWVYKELKGVRNAKPYLTKFGT
                     TLGGAFGVVEMWLSSFGITVPWTLKHTKTDAASTEPASKHKPRVYPKPDGKLSFDKLS
                     SVFISNTNHAEEQPAHLKLLDPSIPIRVNLPEYGEPARLYCPAGVYEVVYADEVARTD
                     PKFVINAQNCVHCKTCDIKDPSQNIVWTTPEGGGGPNYPNM"
     gene            2932..4929
                     /locus_tag="VF633_01115"
     CDS             2932..4929
                     /locus_tag="VF633_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8469686.1"
                     /translation="MMSLRLSLLSFAAPAVILGGVLGLAATGATAQEAPPVSDPAVGQ
                     RSPITVPIGPTQSDPAVLPPAVSAPAEGETEGEPEDGDPATAETPLPPPAAPAVIPIP
                     TIWAPSPVNSEGRTAYGLYLGGRNLVGRSAEADGEAALGARYLAEVQSLTPEQPTVRE
                     QAFTATLLAGNLQDAARMAPSGEGVSPVLAEAGRLVQAIEAYAHGDARAATAIFKTAP
                     IQMPHAPAGLLTQRWIAAAAKDWDFALVAPPATATDTFSQVLRFQRAQLLEVRRRYDE
                     AEAQFKALAANPASQPPFHAGYGGYLERRGRKAEAIAVYDAALGANPDSALLRAARER
                     ASGRNRAPALPNFRDGVSQGLSLAASLASENRANEFAAVYLRLALDVAPSDDMRLLLG
                     GALVGAKLEAASRDALAQIGPGNANAYAAARLQIGASLGREDRNEEALAEFRKAFAAA
                     PQSPDIAYALATQLLQLKRNDEALEVLNGPLLNTPQQPAAVRFVRGAAYEGTGKIAEA
                     ETELTAAVADQPNEPSFLNYLGYLWVDSGTKVAEGAALIARAHAAEPNDGNIQDSLGW
                     AQFRQGQYETAVATLEEAVDKEPANAEINDHLGDAYWQVGRKREAGFQWNRVLTLDPD
                     ADRKAGVEKKLAEGLPPQTPVLGPVAAPSVTPPAALSASGL"
     gene            4931..>5344
                     /locus_tag="VF633_01120"
     CDS             4931..>5344
                     /locus_tag="VF633_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX8469687.1"
                     /translation="MPLTGLAPAKINLFLHVGAVQPDGYHPLASLVAFADIGDTITVE
                     RADALSLTVEGPFGAGLSAEPDNLILRALRALGEAAGIGEPRLHVALDKQLPIAAGLG
                     GGSSDAGTALKLARDVLGLQLDDAALETVAGVVGAD"
BASE COUNT          919 a         1888 c         1691 g          846 t
ORIGIN      
        1 acgggggcga gcatcggcgc gaggagcgcg cggtggtaag cgtacatagg cggcggcccc
       61 ctcgtgcaac gcgtctcccg tctcgattca gggtcggcgc gtctgtctgc gatcggtcgt
      121 caatctgtaa ccatgcctga aaaatcgtca ggcctcaatc acatgaccgt ttataggggc
      181 attccattgg tgagtgcggc gggttgccgc gcccgaggct tggcgagttc gagtatcagc
      241 cgatctcgtc cagccttgca gcaagggaca gcatgtcagc ccacgcctgc ttcttggcct
      301 gaggctgtcg caggaggaag gcgggatgga gcgtcggcag ggcaggggcc gagacgtcgc
      361 cttccgccag tcgccattcg cgccactgcc cgcgcatccg cataatgcct tcatccgtct
      421 gtagcaccga ctttgccgca gcggctccca gcagcagaac gaccttgggc ttcaggatgg
      481 cgaaagcccg ctccacgaag ggggcgcaca ccgcctgttc ctgaggagtc ggtgtgcggt
      541 ttcccggtgg gcgccagaag acggtgttcg tgatgaagac cttgccggtc aaccctgcgg
      601 cctccagcat tcggtccagc aactggccgg ccttgccgac gaaaggttgg ccctgggcgt
      661 cttcatccgc ccccgggcct tcgccgatga tcaggatcgg ggcatctgtc gcgcctcggc
      721 cgaacaccga ctgtcgagcc cccatgccgc gcaggtcgca cgcctcgcaa gtcgccacgg
      781 ccgccgccaa cgcttccagc gtgtccgcac ccgccgccaa tcgtcgcgcc tccgagatcg
      841 cgtcgcggcc gtcgttttgc accacgaccg aggccgtcgc cttggccacc gccttgagtg
      901 caggcggcgg gacgtaggtc cggtcaaccg ctgcgtcttc gtagcaaaca tccactcccg
      961 catcgcgcca gaaggcgagc aggctctcgg ttgcagcgat atccgattgg gagacgttca
     1021 tgaggttcgt acagggataa gccttgaccg ccgcaacgtc acgttccgat aagcgaccaa
     1081 gaacaataat gaccacacag acaaccggga ttgacgagac catggccgag gaagcaatcg
     1141 aaggcggcgc caccactccg gcgcaggaag ccatcctcga caacctgccg cagctggaag
     1201 cgcttcaggg cgtcgagcgc gaggtcatgg aatatgacgt cgtcatcgtc ggcggcggtc
     1261 ccgccggtct gtctgcagcg atccgcctga aacagcgcgc ggcggctgcc gggaccgaga
     1321 tcaacgtcgc tgtgctggag aaatccgccg agatcggcgg ccatatcctg tccggcgccg
     1381 tcatcgatcc taaagcgctg aacgaactgt tccccgactg gaaggaacgc ggcgctcctc
     1441 tggaaacccc cgtcaccaag gacaggttca tgatcctggg cccgatgggc cagttcagcc
     1501 tgccaatggc gctgatgccg cccatgatgc acaaccacgg ctgctacatc gcctcgctcg
     1561 gcaacctgtc ccgctggctg gctgaacagg ccgaggcgct gggcgtcgag gtttatcccg
     1621 gcatggccgc cagccacgtg gtctatgaag agccgtcggg ccgggtgaag ggcgtcgtcg
     1681 ccggcgtctt cggcatcgac aagcacggtc agccgactgg cgagttccag ccgggcatcg
     1741 aactgcatgg caaatacgtc ttcatcgccg agggcgtgcg cggctcgctg gccaaaacca
     1801 tcatcgcccg ccaccaactg gccaaggact ccagcccgca gaaattcggc atcggcctga
     1861 aggagctgtg gcaggtcccg cctgagaagc accagcccgg tctggcccag cactcgaccg
     1921 gctggccgct ggacgagttc accggcggcg gctcgttcat gtaccacttc ggcgacaact
     1981 acgtggccat cggctacgtc gtgcacctga gctacaagga cccgttcctg tcgccgttcg
     2041 acgagttcca gcgcttcaag caccacccgg ccatcgccga acatctggaa ggcggcacgc
     2101 gcatctccta cggcgcgcgt gcgatcaccg agggtggctt ccagtcggtg cccaagctgg
     2161 cgttccccgg cggtgccctg atcggctgtt cggcaggctt cgtgaacgtg ccccgcatca
     2221 agggcagcca taacgccatg aagaccggca tgatggcggc cgacgccgcc tatgacgcgg
     2281 tcatggccgg gcgttcgggc gacgaactgg tcgagtatca agccgcctac gagaagagct
     2341 gggtctacaa ggagctcaag ggcgtccgga atgccaagcc ttatctgacc aaattcggca
     2401 ccacgctggg cggcgctttc ggtgtcgtgg agatgtggtt gtcctccttc ggcatcacgg
     2461 ttccgtggac gctgaagcac accaagaccg atgcggcctc gaccgaaccg gcctcgaagc
     2521 acaagccgcg cgtctacccc aagccggacg gcaagctgtc gttcgacaaa ctaagttcgg
     2581 tgttcatctc caacaccaac cacgccgagg aacagcccgc tcacctgaag ctgctggatc
     2641 cgtcgattcc catccgcgtc aacctgccgg aatatggcga gcccgcgcgc ctctattgcc
     2701 cggcgggcgt gtacgaggtc gtctatgcgg acgaggtcgc gcgcacagat ccgaagttcg
     2761 tgatcaacgc ccagaactgc gtccactgca aaacctgcga catcaaggac ccgtcgcaga
     2821 acatcgtatg gaccacgccc gagggcggcg gcggccccaa ctatccgaat atgtagaaga
     2881 gaccggcgag gccccttgcg ggggagggac aaagtggcga ggttcctcga catgatgtct
     2941 ctccgcctct cgctgctttc gttcgccgcc cctgccgtca ttctcggcgg ggtgctcggt
     3001 ctggccgcca ctggcgcaac tgctcaggaa gcgccgccgg tttccgatcc cgccgtcggc
     3061 cagcgatcgc ccatcactgt cccgatcggc ccgacccagt cggatccagc ggtgctcccc
     3121 ccggcagtgt ctgcgcctgc cgagggtgag accgaaggtg aaccggagga cggcgatccc
     3181 gccacggccg agacgcccct gccgccgccc gcagcgcctg ccgtcattcc cataccgacc
     3241 atctgggcgc cttcgccggt caacagcgag ggccgcacgg cctacggtct ctatctcggt
     3301 ggtcgaaacc tcgtcggtcg ctcggctgag gccgacggcg aggcggctct gggcgcccgg
     3361 tatctggccg aggtccagtc cctcaccccc gaacagccga ccgtgcgcga acaggccttc
     3421 accgccacct tgctggccgg aaatcttcag gacgccgccc gcatggcgcc ttcgggcgaa
     3481 ggggtatcgc cggtactggc agaggccggc cgtctggtgc aggcgatcga ggcctatgcc
     3541 catggcgacg cacgcgctgc gactgcgatc ttcaagaccg cgcccatcca gatgccccac
     3601 gcgcccgccg gtctgctgac ccagcgctgg atcgccgccg ccgccaagga ttgggatttc
     3661 gcactggtcg ccccgcccgc gaccgcgacc gacaccttca gtcaggtcct gcgcttccag
     3721 cgcgcgcagt tgctcgaagt ccgtcgccgc tatgacgagg ccgaggccca gttcaaggct
     3781 ttggccgcaa accccgcgtc tcagccgccc ttccatgccg ggtacggcgg ttatctggag
     3841 cggcgcgggc gcaaggccga ggccatcgct gtctatgatg cggccctcgg agccaatccc
     3901 gatagcgccc tgctcagggc cgcccgcgag cgcgcttcgg gtcgcaaccg ggctccggcg
     3961 ctgccgaact tccgtgacgg cgtttcgcag ggcctgagcc tcgccgcgtc gctggcgagt
     4021 gaaaaccgcg ccaacgagtt cgcggcggtc tatctgcggc tcgcgctcga cgtagcaccc
     4081 agcgacgaca tgcggttgct actcggtggc gcgctcgtcg gcgccaagct cgaggccgcc
     4141 agccgcgacg ccctggccca gatcggcccg ggcaacgcga acgcctatgc cgccgcccgc
     4201 ttgcagatcg gcgccagcct cggtcgcgaa gaccgcaatg aggaagcgct ggcggaattc
     4261 cgcaaggcct tcgccgccgc cccacaatcc ccggacatcg cctacgcgct ggcgacccag
     4321 ctgctgcagc tcaagcgcaa tgacgaggcg ctggaagtcc tcaacggccc gctcctcaac
     4381 acgccgcaac agcccgccgc cgtccgtttc gtgcgcggcg ccgcctatga gggcacgggc
     4441 aagatcgccg aggcggagac cgaactaacc gccgccgtcg cggaccagcc caacgagccg
     4501 tccttcctca actacctcgg ctacctctgg gtcgacagcg ggaccaaggt cgccgaaggc
     4561 gcagccctga tcgcgcgagc ccacgccgcc gagcccaacg acggcaacat ccaggactcg
     4621 ctcggctggg cccagttccg tcagggtcag tacgagaccg ccgtcgccac cctcgaggaa
     4681 gcggtcgaca aggaacccgc caacgccgag atcaacgacc acctcggcga cgcctactgg
     4741 caggtcggtc gcaagcgcga ggcgggcttc cagtggaacc gcgtcctgac gctcgacccg
     4801 gacgcggatc gcaaggccgg ggtcgaaaag aagctggccg agggcctgcc gccacagacc
     4861 cccgttcttg ggccggtcgc cgcgccttcg gtgacgccgc cggcagccct ctcggcctcc
     4921 ggactgtaga gtgccgctaa ccgggctcgc gcccgccaag atcaatctgt tcctgcacgt
     4981 cggggcggtg cagcccgacg gctatcatcc gctcgccagc ctcgtcgcct tcgccgacat
     5041 cggcgacacg atcaccgtcg agcgtgcgga cgccctgtcc ctgacggtcg agggcccctt
     5101 cggcgcaggc ctgtcggctg agccggataa tctgatcctc agggcgttgc gggcgttggg
     5161 cgaggctgcc ggaatcggtg agccacgcct ccacgtcgct ctcgataagc agcttcccat
     5221 cgcagccggt ctcggcggcg gatcatccga tgcaggcaca gccctgaagc tggcgcggga
     5281 tgtcctcggg cttcagctcg acgacgctgc tctggagacc gtcgcaggcg tcgtcggcgc
     5341 ggac
//
LOCUS       DASVTM010000046         8205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_696977,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000046 DASVTM010000000
VERSION     DASVTM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696977"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VF633_01125"
     CDS             complement(<1..843)
                     /locus_tag="VF633_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX8469688.1"
                     /translation="MKPISRPLLYAGLIALLLILSGISLIAGKVWVPFSAWLQPDVDP
                     RWAIVFELRLPRTILGIAVGAALGLSGAVLQGYTRNPLADPGVLGVSAMAALGAVLTL
                     YYGVTAVAPWALPVAAISGALIGVALLMLLSGTASSLVTFVLAGAILNILSSAGVSLA
                     LSLAPNPWAVNEIVDWLMGSLADRSFEEVQMALPFILVGCGLLLTTGRALDALTLGEV
                     GARSLGVSMPVTRWSIALGVALAAGASVAVTGVIGFVGLVTPHLLRPLVGARPGALLV
                     PSILG"
     gene            complement(840..1733)
                     /locus_tag="VF633_01130"
     CDS             complement(840..1733)
                     /locus_tag="VF633_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8469689.1"
                     /translation="MSQPLHRRTVMLGGLAMTAGCNVAPAASPPTSTGRPQRIVSLNP
                     CLDVILIHVADRAQIAALSHYARDSYGSTFAPLARTLPFTYETAEEVLSLSPDLVMTG
                     RHSSLATRNALDRLKISTALFGVPETIAESFEQVREVATLVGHPERGEALIRQIEAAL
                     AEAAPRPGTPPLSALVFMPGGFASGPGTLMDEMMRRMGLENAASRYGLKSSRRVPLER
                     IIADPPDVLLAGEAWPGAPNWALRVMTHPALSHVAGRMERATFPERLLFCGGPVLIQT
                     AAALSRAREHALSNRKAANRA"
     gene            complement(1730..2350)
                     /gene="cobO"
                     /locus_tag="VF633_01135"
     CDS             complement(1730..2350)
                     /gene="cobO"
                     /locus_tag="VF633_01135"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX8469690.1"
                     /translation="MTETPELPKTDAEHTEAMRTLQAERRELMRFKTEERGLLIVNTG
                     DGKGKSTAGFGMVARALGWGQKVGIVQYIKGKWITGERQFFRKFPDQVRYEVMGEGFT
                     WDTQDRSRDIAAARAAWAISLEMIADPTLNLVLLDELNIALRYDYLDIDEVVAGLVAR
                     PEGKHVCVTGRNAKPQLLEVADLVTEMTLVKHPFEQGVKAQRGIDF"
     gene            complement(2347..3324)
                     /gene="cobD"
                     /locus_tag="VF633_01140"
     CDS             complement(2347..3324)
                     /gene="cobD"
                     /locus_tag="VF633_01140"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HEX8469691.1"
                     /translation="MAGPIPHGGRLAAAQADHPLAPLPWLDLSTGINPDGWRGARASD
                     AALTVLPDPADTAALEAAAALAFGCDPDRVAAVSGAEAGLRLLPALLDVARVAVASPT
                     YGSHADAWRQARVETVEAERTHLFQQPTDTLVIVNPNNPDGAITPGAELVAAAERRWL
                     IIDESFVDTDPAMSAIPHMTDRVVVLRSFGKFYGLPGVRLGFVVASPALAARIRQRTG
                     DWPVGADAIVMGQAAYADTDWADRTRALLLQEAARLDERLIASGFTIVGGTSLFRLVR
                     CEDAPGRAQRLAEQGVLVRTFPHDPELIRFGLPGQANWRRLETALEMSR"
     gene            complement(3333..5318)
                     /locus_tag="VF633_01145"
     CDS             complement(3333..5318)
                     /locus_tag="VF633_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469692.1"
                     /translation="MRPELYLGAATAALLLTATAAAAQTATLTDDPALLDDVIVTANR
                     SPQAADRVGQSVTVLTTKQIEGSQAVGVVDLLVRTPGVSLSRNGGIGASTSLRIRGAE
                     SDQTVVVIDGVKLNDPSATGSGYNFGNLLVSDIARIEVLRGAQSTLWGSQAIGGVVNL
                     ITAEPTRPFQASLTAEAGSRGTHYLHGVIGGVTERVNWRIAASHYQTDGFSTFARGAE
                     KDGYENTGLSGRLNLRLTEDVSVDLRAVYSNGRVEIDGFPAPTFSFGDTREYGTTKDL
                     VTYAGLNFDLFEDRLKNRIAYAWTQTDRQNFNPAQTVTNLTFDARGENKRFEYQGVFD
                     IAQGWTATFGAEHEDSEFRTASPSAFDANPTPRSNSVSIDGAYLQVQGEVIDGLTLTG
                     GVRRDEHDTFGGKTLGQAAAAWSLNDGSTILRASFGQGFKAPSLYQLYSEYGNTTLSP
                     EEADSWDAGIEHGLFGRRLIVSATWFDRDTTNQIDYASCNGTETVTSVPLCFVPVTVN
                     GVVTNQPRFGYYDNIAHTKAKGFELAAAAYLGQIEVQANYTYTDATNESAGANFGRQL
                     ARRPQDTANLSATYLWPFRLSTTVAVQYAGESYNNASNSQRLEPYTLVDMRASYPLNE
                     TVELYGRVENVGDEKYETTRDYGVAGRGTFVGVRARF"
     regulatory      complement(5468..5675)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5712..6227
                     /gene="cobU"
                     /locus_tag="VF633_01150"
     CDS             5712..6227
                     /gene="cobU"
                     /locus_tag="VF633_01150"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX8469693.1"
                     /translation="MPTTLVLGGARSGKTAYAQQRAESIAAPMHRRLVMIVTAQAFDD
                     EMQDRIARHRADRDDRWTTVEAPLDLVGAIAALTAEDVAVVDCLTLWLSNLMGSEADI
                     QAAFGGLVDAVVACPADLLLVSNEVGWGIVPDNAMARAFRDHAGRLHQRLAKVAEETV
                     LVVAGLPLRLN"
     gene            complement(6224..6556)
                     /locus_tag="VF633_01155"
     CDS             complement(6224..6556)
                     /locus_tag="VF633_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469694.1"
                     /translation="MNRPRMTRSMSTDPGANKVRWIGALALAALAYWSWTHRKPSFTQ
                     RAEAAAKASGVIEVSFDPARQRGQMTVICGNADGKLVVYREPGGFARDDGSLAFITLY
                     RDGCGPKT"
     gene            complement(6556..7113)
                     /locus_tag="VF633_01160"
     CDS             complement(6556..7113)
                     /locus_tag="VF633_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469695.1"
                     /translation="MTGLPLLPDRTCGGCVECCRFIPLDLPELAKPTGELCAYCVDGA
                     GCGVHEIRPRTCRTWFCVWRVVELSDDWRPDRSGVIVRPDGLTDGEITLYVIDRSDFL
                     LSDDFFDTVVHWLDNGIRLAFSVPGPVGTFPVRAVVTDYLKQAVAAGDRDLFNQLVAR
                     SLDSLANHDWQPDGVVAHYAVGEVS"
     gene            complement(7177..>8205)
                     /locus_tag="VF633_01165"
     CDS             complement(7177..>8205)
                     /locus_tag="VF633_01165"
                     /inference="COORDINATES: protein motif:HMM:NF021382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX8469696.1"
                     /translation="LADRSVELIDRDGTDSADPVAESRRFLAARRNSFPILDDTAERL
                     AAGVVEAGGLAAFLSAKHGLRTRRMPPDVMVGSIRRYDLHRREILLDDTLDTASQTFQ
                     LALQTAYLEMRADIDAVVAGGVFATEGGERLTRRALASYAAAAMVMPYTAFARAAETR
                     RYDVEALARQFGTSFEQTAHRLTTLQKPGQERVPFFFIRVDPAGNVSKRLDGAGFSFA
                     RHGGGCPLWSVHSAFRTPREIVTQWLELPDGQRFFSIARTVTQGGGGFGSPRVERAIA
                     LGCAAEHAHKLIYTQGLPEPLPTPIGVTCRLCHRLECPARSAPPIGRQILPDDIRRTS
                     TPFGFSDA"
BASE COUNT         1423 a         2755 c         2699 g         1328 t
ORIGIN      
        1 gccgaggatg ctgggcacaa gcaatgcgcc cggcctcgcc ccgaccagcg gccgcaacag
       61 atgcggcgtc accagcccaa cgaacccgat caccccggtc accgcgacgc tggccccggc
      121 cgccaaagcc acgccgagtg cgatgctcca gcgggtcacc ggcatggaga cgcccagcga
      181 ccgcgcccca acttcgccga gcgtcagggc gtccagcgcc cgaccggtgg tcagcaacag
      241 gccgcagccc acgaggatga agggcagcgc catctgcacc tcctcgaagc tgcggtcggc
      301 cagcgatccc atcaaccagt cgacaatctc gttcaccgcc cacgggttcg gggccagcga
      361 cagggccagc gacactcccg cgctcgacag gatgttcagg atcgcgcccg ccagcacgaa
      421 ggtcacgagg ctggaggccg tgccggacag cagcatcaac aacgccaccc cgatcaacgc
      481 tccgctgatc gccgccaccg gcagggccca cggtgccacc gccgttacgc cgtaatagag
      541 agtcagcaca gcgccgagag ccgccatggc cgagacgccg agcacgcccg gatcagccag
      601 cgggttgcgg gtgtatccct gcagcaccgc gcccgagaga cccagagctg cgccgacggc
      661 gatgcccaga atggtgcgcg gcagacgcag ttcgaagacg atggcccagc gcggatcgac
      721 gtcgggctga agccacgcgc tgaacggcac ccagaccttg ccggcgatca gactgatgcc
      781 tgacaggatc agcagcaggg cgatcaagcc cgcatagaga agagggcggc ttatcggttt
      841 catgctcggt tcgccgcttt tcggttgctc aaggcgtgtt cgcgcgctcg cgacagggcg
      901 gcggccgtct ggatcaggac cgggccaccg cagaacagca gccgctctgg gaaggtcgcg
      961 cgctccatcc ggccagcgac gtgcgagagc gccggatggg tcatgacccg caaagcccag
     1021 ttcggggctc cgggccaggc ttccccggcc agaaggacgt cgggcgggtc ggcgatgatc
     1081 cgctccagcg gcacgcgccg ggagctcttg aggccgtagc gagaggctgc attctcaagt
     1141 cccattcgac gcatcatctc gtccatcagg gtcccgggtc cggaggcgaa gccgcctggc
     1201 atgaagacca gcgccgacaa cggcggcgtt ccgggcctcg gcgcggcttc cgccagcgcg
     1261 gcctcgatct ggcggatcag ggcctctccg cgctcgggat ggccgaccag tgtggcgacc
     1321 tcgcgcacct gttcgaagct ttcggcgatg gtctcgggca cgccgaacag ggccgtcgag
     1381 atcttcagcc ggtccagcgc attgcgcgtc gcgagcgacg aatgccgccc ggtcatcacc
     1441 agatcgggag agagggaaag cacctcttcg gccgtctcgt aggtgaacgg cagggtccgg
     1501 gccaagggcg cgaaggtcga accatagcta tcgcgagcat agtggctgag cgccgcgatc
     1561 tgcgcccggt cggcgacgtg gatcaggatc acgtcaaggc aagggttcag cgagacgatc
     1621 cgctggggac ggcccgtcga tgtcggaggc gacgctgccg gcgcgacgtt gcagccggcg
     1681 gtcatggcca gcccgcccaa catcaccgtc cgtcggtgca gcggctggct caaaagtcga
     1741 ttccccgctg ggccttcacg ccctgctcga acggatgttt caccagcgtc atctcggtga
     1801 ccagatcggc cacctccagc aactgcggct tggcgttacg gccggtgacg cagacatgct
     1861 tgccctcggg ccgcgcgacc agtccggcga cgacctcatc gatgtccaga tagtcgtagc
     1921 gcagggcgat gttcagctca tccagcaaca ccagattcaa ggtcggatcg gcgatcatct
     1981 ccagcgagat ggcccacgcc gccctggccg ccgcgatgtc gcgcgaacgg tcctgggtgt
     2041 cccaggtgaa gccttcgccc atcacctcgt agcgaacctg atccgggaat ttgcggaaga
     2101 actgccgctc gccggtgatc catttgccct tgatatactg gacgatgccg accttctggc
     2161 cccagcccag ggcgcgggcg accataccga agccggcggt ggacttgccc ttgccgtcgc
     2221 cggtgttgac gatcagcagg ccgcgctcct cggtcttgaa gcgcatcagt tcgcgacgtt
     2281 cggcctgcag agtcctcatc gcctcggtgt gttcggcgtc ggttttgggc agttcgggcg
     2341 tctcggtcat cgcgacatct ccagagcggt ttcaagccgc cgccaattcg cctgtcccgg
     2401 caagccgaaa cgaatgagtt cgggatcgtg cgggaaagtt cgcaccagta caccctgctc
     2461 cgccagccgc tgcgcgcgac ccggagcgtc ctcacagcgc acgagccgaa acaggctggt
     2521 tccaccgacg atcgtgaagc cggatgcgat cagacgctca tccagtcggg ccgcttcctg
     2581 caacagcaag gcgcgcgtcc tgtccgccca gtcggtatcg gcataggcgg cttgccccat
     2641 gacaatcgca tccgcgccga ccggccagtc tcctgtccgt tggcgaatcc gtgcagcgag
     2701 cgcgggactg gccactacga accccagccg cacgccaggc aatccataga attttccgaa
     2761 cgaccgcagc accacgaccc ggtcggtcat gtgggggatc gcgctcatcg ccggatccgt
     2821 atcgacgaag gactcgtcga tgatcagcca gcgacgctcc gccgccgcga ccagctctgc
     2881 gcccggcgtg atcgcaccgt ccggattgtt gggattgacg atcacgaggg tgtcggtggg
     2941 ctgctggaac aggtgcgtcc gctcggcctc taccgtctcg acccgcgcct gacgccaagc
     3001 gtcggcatgg ctgccgtacg tcggcgaggc cacggcgacc cgtgcgacat cgagcaaagc
     3061 aggcaggagc cgcagccccg cctcagctcc ggaaacggcc gcgacccgat caggatcaca
     3121 accgaaagcc aaggccgcag ccgcctcgag cgctgcggtg tccgctggat cgggcaggac
     3181 cgtcagggcg gcgtcagaag cacgcgcacc cctccagcca tccgggttga tgcccgtcga
     3241 caggtcaagc caaggcagcg gcgcaagcgg gtggtccgcc tgcgccgccg ccagtcgtcc
     3301 accgtgagga atgggaccgg ccatgttacg tcctagaagc gggcgcgaac gccaacgaag
     3361 gtcccgcgtc ccgcaacgcc gtagtcgcgg gtggtttcgt atttctcgtc gccgacattc
     3421 tcgacccggc catagagttc gaccgtttcg ttcagtggat acgaggcccg catatcgacg
     3481 agggtgtagg gctccaggcg ctgtgagttg gaggcgttgt tgtagctttc gcccgcgtac
     3541 tggaccgcca ccgtcgtcga caggcggaac ggccagagat aggtggccga aaggtttgcc
     3601 gtgtcctgcg ggcggcgagc cagttgcctg ccgaagttcg ccccggccga ctcgttcgtg
     3661 gcgtcggtgt aggtgtagtt ggcctgcacc tcgatctggc cgaggtaggc tgcagcggcc
     3721 agctcgaagc ccttcgcttt ggtgtgggcg atgttgtcgt agtagccgaa gcgaggctgg
     3781 ttggtgacca cgccgttgac cgttaccggc acgaagcaca gcggtacgga cgtaaccgtc
     3841 tcggttccat tgcaggaagc gtagtcgatc tggttggtcg tatcgcgatc gaaccaggtc
     3901 gctgacacga tcagacgcct gccgaacaga ccgtgttcga taccggcatc ccagctgtcg
     3961 gcttcttctg gcgacagggt cgtgttcccg tactcgctgt agagctgata caggctcggt
     4021 gccttgaagc cttggccgaa gctggcgcgc aggatggtcg agccatcgtt caacgaccat
     4081 gccgcagcag cctgaccgag ggtcttgccg ccgaaggtgt catgctcgtc acgacgcacg
     4141 ccaccggtca gggtaaggcc gtcgatgacc tcgccctgca cctgcagata ggcgccgtcg
     4201 atgctgaccg agttggaacg gggggtcgga ttagcgtcga aagccgatgg cgaagccgtg
     4261 cggaactcgc tgtcctcatg ctcggctccg aacgttgccg tccagccttg ggcgatgtcg
     4321 aacacacctt gatattcgaa gcgcttgttc tcgccgcgcg catcgaacgt caggttcgtc
     4381 acggtttggg ccgggttgaa gttctggcgg tcggtctggg tccaggcata ggcgatgcgg
     4441 ttcttcaggc ggtcttcgaa caggtcgaag ttcagcccgg cgtaggtcac cagatccttc
     4501 gtcgttccgt attcgcgcgt gtcgccgaac gagaacgtcg gcgccgggaa gccgtcgatc
     4561 tcgacgcggc cgttcgagta gacggcgcgc aggtcgaccg agacatcctc ggtcagcctc
     4621 aggttcaaac ggccgctaag gccagtgttc tcatacccgt ccttctcggc tccccgggcg
     4681 aaggtcgaga agccgtcggt ctggtagtgc gaggccgcga tgcgccagtt gacgcgctcg
     4741 gtcacgccgc cgatgacgcc atggagatag tgggtgccgc gcgatccggc ctcggcagtc
     4801 agcgaggcct gaaaaggacg agtcggttcc gcggtgatca ggttgaccac cccgccgatg
     4861 gcctgactgc cccacagggt cgattgcgct ccccgcagga cttcaatccg ggcgatgtcg
     4921 ctgaccagca ggttgccgaa attatagccg ctgcccgtcg ccgacggatc gttcagcttg
     4981 acgccgtcga tgacgaccac ggtctggtcc gactcggcgc cgcggatgcg gagcgaggtc
     5041 gacgcgccga tgccaccgtt gcgggacaga ctgacgccgg gggtgcgcac cagaaggtca
     5101 acgacgccga cggcctggct gccctcgatc tgcttcgtgg ttagtacggt aaccgactgg
     5161 ccgacacgat cagcggcctg tggcgagcgg ttagcggtca cgatgacgtc gtccagcaag
     5221 gccggatcgt cagtcagcgt cgccgtttgc gcggcagccg cagtagcggt cagcagaagc
     5281 gcggccgtgg cggcgcccag gtaaagttca ggtctcatag cgggtccaat cccttgctcg
     5341 acaacgagct ggccgaacga ggccgtgatc ggatgcggac gcgcccggtc acgaccgcgc
     5401 tcacacgcag cccaacagac gtcgtcattc gggtagatcc cgttccgctc ggcacaccac
     5461 gtccgctcga aagacgacca cgacgggcag gtctcctggc tcacgggtca acaccggtgc
     5521 gcaccgcctt cccagaccct ccagctttcg ccttcggaac ccagtggcta ttggtgcgcg
     5581 ggctcgccgc ttacagttgc gtgggcagtc cgggattttc acccgagttc ccttttcatc
     5641 cccatcgctg gggaacctgt cgcgaggcgg ccaataggct aaagcgtcac tacaggcaat
     5701 ggagcgtctc gatgccgacc acactcgttc tcggcggggc ccgctcgggc aagacggcgt
     5761 atgcacagca gcgcgcggaa tccatcgccg cgcccatgca ccggcgactg gtgatgatcg
     5821 tcaccgctca ggctttcgat gacgagatgc aggaccggat cgctcgccat cgggcggatc
     5881 gtgacgatcg ctggacgacc gtggaagcgc cgcttgatct ggtcggcgcc atcgcggccc
     5941 tgacggctga agacgtcgcc gtagtggatt gcctgacgct ttggctcagc aacctgatgg
     6001 ggtccgaggc cgatatccaa gccgcgttcg gcggcttggt cgatgcagtg gtcgcctgtc
     6061 cggcggacct gctgctggtc tccaacgagg tcggctgggg gatcgtgccg gacaacgcca
     6121 tggcccgggc gttccgcgat cacgcagggc ggttgcacca gaggctggcc aaggtcgccg
     6181 aggagactgt gctggtcgtg gctggcctgc cgctgagact gaactaggtc ttcggcccgc
     6241 agccgtcgcg gtacagggtg atgaaggcca gagacccgtc gtcgcgggcg aaaccgccgg
     6301 gctcacgata gacgaccagc ttgccgtcgg cgttgccgca gataaccgtc atctggcccc
     6361 gctgccgtgc cggatcaaag ctgacttcga tgactcctga cgccttggcc gccgcctccg
     6421 cccgctgggt gaaactcggc ttgcgatggg tccagctcca gtaggcaagg gctgcgagcg
     6481 ccagcgctcc gatccagcgg accttgttgg cgccgggatc ggtcgacatc gaccgggtca
     6541 tgcgaggccg gttcatcacg acacctcgcc gacggcatag tgggcgacga cgccgtcggg
     6601 ctgccagtcg tggttcgcca gactgtccag cgaccgcgcc accaactggt tgaacaggtc
     6661 gcgatcgccc gccgccaccg cctgcttcag atagtctgtg accacggccc gtactggaaa
     6721 ggtcccgacc ggccccggca cgctgaaggc cagccggatg ccgttgtcga gccaatgaac
     6781 caccgtatcg aagaagtcgt ccgacagcag gaagtcgctg cggtcgatga cgtagagggt
     6841 gatctccccg tccgtcagcc cgtcgggtcg gacgatgacg ccgctgcggt cgggccgcca
     6901 gtcatcggac agctcgacaa cccgccagac gcagaaccag gtcctgcagg tccgcggccg
     6961 gatctcgtgg acaccacacc ccgctccgtc gacgcaatag gcgcacagct ccccagtcgg
     7021 cttggccagt tccggcaggt cgagtggaat gaaccggcag cattccacgc agccgccgca
     7081 ggtgcggtcc ggcaggagcg gaagcccggt catgatcgat cgctcgccat gccccgacct
     7141 aaacccgcgg tggggcagag gccagtcctg agcgcctcaa gcatctgaaa acccgaaagg
     7201 tgtgctcgtc cgacggatat cgtcgggcaa gatttggcga ccgatcggcg gcgccgaccg
     7261 ggctggacac tccagcctgt gacacaggcg acaggtgacc cctatcgggg tcggcaaagg
     7321 ttccggcaga ccttgcgtat agatcaactt gtgagcgtgt tcggccgcgc agcccagggc
     7381 gatggcccgc tctaccctcg ggcttccgaa cccgccgcca ccctgcgtca ccgttcgggc
     7441 gatggagaag aagcgctggc catcgggcaa ctccagccat tgggtcacga tctcgcgcgg
     7501 ggtccggaaa gctgaatgca ccgaccacag cggacagccg ccgccgtgcc gggcgaacga
     7561 gaaccctgct ccgtccagcc gcttggacac attgcccgcc ggatcgacgc ggatgaagaa
     7621 gaacggcacc cgctcctgtc cgggcttttg cagggtcgtc agccggtgcg ccgtttgttc
     7681 gaagctggtc ccgaactggc gcgccagcgc ctcgacatca tagcgccgcg tctcagccgc
     7741 gcgggcgaag gcggtgtagg gcatgaccat cgccgccgca gcatagctgg ccagcgcccg
     7801 ccgggtgagc cgctcgccgc cctcggttgc gaacaccccg cccgcgacca ccgcgtcgat
     7861 gtcggcccgc atctccagat aggcggtctg caacgcgagt tggaatgtct gactggcggt
     7921 atcgagcgtg tcgtcgagca ggatctcgcg ccggtgcagg tcgtagcgcc ggatcgagcc
     7981 gaccatgacg tcgggcggca tccgccgggt ccgcagcccg tgtttggcgc tcaggaaagc
     8041 cgccagaccg ccggcctcga cgacccccgc cgccagtcgt tccgccgtgt cgtcgaggat
     8101 cggaaaactg ttccgccgcg ccgccagaaa ccgccgggac tcggccaccg gatcggcgct
     8161 gtcagtccca tcgcgatcga tcagctcgac gctgcgatcg gcgag
//
LOCUS       DASVTM010000047         6743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_5222, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000047 DASVTM010000000
VERSION     DASVTM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5222"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VF633_01170"
     CDS             complement(<1..544)
                     /locus_tag="VF633_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469697.1"
                     /translation="MKTRLIKPVMLAAAAALALAACATATPYQAAGTGSARGGYAEQR
                     LESDRFRVSFSGNSVTSREQVEMGLLLRSAELTRESGFDWFSTVNRATDRDTRFQTLG
                     TGFNDPFYGRYSPYWGPSWRFYGGGGWSPWGNPWGRGWGGFNDFDVRQIDRYEATSEI
                     IMGRGPKPAGDPNAFDAAEVI"
     gene            complement(623..1258)
                     /locus_tag="VF633_01175"
     CDS             complement(623..1258)
                     /locus_tag="VF633_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX8469698.1"
                     /translation="MTSSLPVDPHLYAAFLGVMAVMAITPGPANLFSVATGMERGKAA
                     ALLGVVGMNSATLVWFLAAALGLGALVAAFPQVFKLVAIGGALYVGWLGVKALRGAFA
                     AESAPKAVVISRDRSAIFDGFMVQIANPKVILFFTAVLPPFLDLNRPIVPQLVLFAMA
                     TIGMDVLSMSAYGLGGAALARRMSEPRFRRGFGIFVGVLLMLAAVLIVSRL"
     gene            complement(1372..1965)
                     /locus_tag="VF633_01180"
     CDS             complement(1372..1965)
                     /locus_tag="VF633_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469699.1"
                     /translation="MIRILSLAAALAILPFSAAVAEENKTQSEAAIEAAASAFEARME
                     AFGTRAEAIGDDKSLTEAQREAKIATLWSEYQPEVAVFTAVVSQHASKIASEALANID
                     VDAMITAALAQPEVQAQVQSAMAGASGMAANGAWAQNDPEQMVTYGLMAQYGVDQAMD
                     AATGDVAAAEEDARAAKTEARAAENEARPNPASHSHH"
     gene            complement(2043..2531)
                     /locus_tag="VF633_01185"
     CDS             complement(2043..2531)
                     /locus_tag="VF633_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469700.1"
                     /translation="MRTLTTAAVAAAALMMAAPAFAQTAPAPAPTPAPAPAAPAAAGP
                     DEDAVEAASAAFEAQIEQMTTEIQSAKTAAGTDTAKANTDADAIVARYKPKADAFAEM
                     VKAFIATQPVPPEAQPQVTAAIDQIRGIPKMARDHAMNPEPAPVATPAPEAATPTPPA
                     AN"
     gene            complement(2585..3820)
                     /locus_tag="VF633_01190"
     CDS             complement(2585..3820)
                     /locus_tag="VF633_01190"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX8469701.1"
                     /translation="MSKASDKPVKKVVLAYSGGLDTSIILKWLQTEYNAEVVTFTADL
                     GQGEELGPAREKALKMGIKPENIFVDDLREEFVRDFVFPMFRANTVYEGQYLLGTSIA
                     RPLIAKRQIEIARMVGADAVCHGATGKGNDQVRFEIGYYALEPDIRVIAPWREWEYRS
                     REALLEFAEKNQIPIAKDKRGEAPFSVDANLLHSSSEGKVLEDPAVEAPEFVHQRTIA
                     PEDAPDKAHVFTMTFEKGDPVAIDGVAMSPATILTKLNELGHANGVGRLDLVENRYVG
                     MKSRGVYETPGGTILLAAHRGIESITLDGGSMHLKDQLMPKYAELIYNGFWFAPEREM
                     LQAAIDYTQQKVAGTVTIKLYKGNVSIIGRESPYSLYDQDLVTFEEGVQAYDHNDAAG
                     FIKLNALRLRVLAKRNARG"
     gene            complement(3926..4804)
                     /locus_tag="VF633_01195"
     CDS             complement(3926..4804)
                     /locus_tag="VF633_01195"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519125.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX8469702.1"
                     /translation="MSSTFPRVRKAVLPVAGLGTRVLPAAKTTPKNMLNVFDRPILSH
                     IVEEARAAGIEHFIFVVGRGQSSVEDYFDHAYEMEAILKSKGKNEILEMVVADLPKPG
                     EMSFVRQMAPLGLGHAVYCARDLIGDEPFAVILADMLMMADTPALKQAIDAHEKTGGN
                     IVVVEQAPEGETHKYGIVALDGQDGRLNRMTGMVEKPPLGTEPSNLFISGRYILTPDI
                     FPLLADQQSGAGGEIQLTDAMARLMKVQDFHALEYEGDTYDCGDPVGLLRANVAYALK
                     HAKLGAAAREAIAALL"
     gene            complement(4914..6734)
                     /gene="glmS"
                     /locus_tag="VF633_01200"
     CDS             complement(4914..6734)
                     /gene="glmS"
                     /locus_tag="VF633_01200"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX8469703.1"
                     /translation="MCGIIGIVGKAPVSDRLIDSLKRLEYRGYDSAGIAVVVGGKVER
                     RRAQGKIKNLEALLAAEPLAGQNGIGHVRWATHGAPNLKNAHPHTAGRVTLVHNGIIE
                     NFAELKAELAAAGRTFESDTDTEVIAHLIDAELATGLAPLEAFKAALDRLTGAYALAV
                     LIEGADDLILGARRGSPLVVGEGQGEMFLGSDALAVGPFTNRVIYLEEGDFVAIDHDS
                     ARIFDASGAPVVRAVKVVPASAVMMEKGNYRHFMEKEIHDQPEGCQRTIAAYVDALTA
                     RAAMPGDVDFKALERIQIVACGTSYIAGMIGKYLIEQLADLPVDVEIASEFRYRQPAL
                     RPSSLVIAMSQSGETADTLAALRYCKARGMKSAVIVNAQESTMAREVDVVWPIHCGPE
                     IGVASTKAFTAQVSVMIALAVAAAKARGRIDAAEEQRLVKVMLEAPRLIAEAIGLEDA
                     LKEIAFDIAKARDVLFLGRGPMSALALEGALKLKEISYIHAEGYAAGELKHGPIALVD
                     DQTPIIILAPYDSYFEKSASNMSEVMARGGQVVFITDPEGAKHAPAGARVVVTAPASD
                     PLVATLVMSAPIQLLAYHVAVVKGADVDQPRNLAKSVTVE"
BASE COUNT         1136 a         2159 c         2246 g         1202 t
ORIGIN      
        1 agatcacttc cgctgcgtcg aaggcgttgg gatctccggc aggcttggga ccccggccca
       61 tgatgatctc cgaggtcgct tcgtaacggt cgatctgacg gacgtcgaaa tcgttgaagc
      121 caccccagcc acggccccac gggttacccc aagggctcca gccgccgccg ccgtagaagc
      181 gccacgacgg accccagtag ggcgagtaac ggccatagaa cggatcgttg aagccggtgc
      241 ccagggtctg gaagcgggtg tcacgatcgg tcgcgcggtt gacggtcgag aaccagtcga
      301 aaccgctctc gcgggtcagt tcggcggagc gcagcagcag ccccatctcg acctgttcgc
      361 gcgacgtcac cgaattgccc gagaagctga cccggaaacg gtcgctttca agcctttgct
      421 cggcgtagcc gccgcgtgcg ctgccggtgc ctgcagcctg gtagggagtg gccgtggcgc
      481 aggcggccaa ggcgagggcc gcggcggcgg ccagcattac gggctttatc aaacgcgttt
      541 tcatcactga actccatgac ggacatagcc gccttttctg gtgaacgggc gagctgcagg
      601 tccgttcccc gcaggcaacc cgttataatc gtgacacaat caacacggcg gcgagcatga
      661 gcagaacgcc gacgaagatc ccgaaaccgc gccggaaccg gggttccgac atacggcggg
      721 caagcgccgc tcccccgaga ccgtaagcgc tcatcgagag cacgtccatg ccgatggtgg
      781 ccattgcaaa caacaccagt tgcggaacga tcggacggtt caaatcgagg aaaggcggca
      841 ggacggcggt gaagaacagg atgaccttcg ggttggcgat ctggaccatg aacccgtcga
      901 atatggcgct gcgatcgcgc gagatgacga cggccttggg cgcgctttca gcggcgaagg
      961 cgccgcgcag ggccttgacg cctagccagc cgacatagag cgcgccaccg atggcgacca
     1021 gcttgaacac ctgcggaaac gccgccacca gcgcgcccag tcccagggcc gccgccagga
     1081 accagacaag cgtcgcgctg ttcatcccca ccacgccgag caaggccgcc gccttgcccc
     1141 gctccatccc ggtggcgacc gaaaacaggt ttgccggtcc cggcgtgatc gccatcaccg
     1201 ccatcacccc gaggaaggct gcatagaggt gcggatcgac gggaaggctg ctggtcatga
     1261 gtgggctttc cggcaagcgc gggcctcacg tcaagagcgc tggcaggtca agcggtccgc
     1321 agcggggaga gctcactgtg atccagagca actgcccttt gcgggaaagc ctcagtgatg
     1381 agaatgcgaa gccggatttg ggcgcgcttc gttttctgca gcccgcgcct cggttttggc
     1441 tgccctggcg tcttcttcag ctgcggccac atcacccgtc gcggcgtcca tggcctgatc
     1501 aacgccgtat tgggccatca ggccgtaggt gaccatctgt tccgggtcgt tctgggccca
     1561 ggcgccgttg gcagccatgc ccgatgcccc agccatggcg gactggacct gagcctgaac
     1621 ctcgggttgg gccagcgctg ccgtgatcat ggcgtcgacg tcgatgttgg ccagcgcctc
     1681 ggaagcgatc ttgctggcgt gctggctgac gacagccgtg aagaccgcga cctcgggttg
     1741 atactcggac cacagggtgg cgatcttcgc ttcgcgttgg gcttcggtca gggacttgtc
     1801 gtcgccgatg gcctcggcgc gcgtgccgaa cgcctccatc cgggcctcga aggcgctggc
     1861 ggcggcttcg atcgcagcct ccgactgggt cttgttctct tcggcgactg cggccgagaa
     1921 cggcaggatg gcgagagcgg cggcgagact caggatgcgg atcatggcgc ggcttccttg
     1981 cagatgacgc cgccaaaatc ctccctgcac cccgctatcg caagttactt cgccgtaagg
     2041 gcctagtttg cggccggcgg tgtcggggtt gcagcctcag gagccggggt cgcgacaggg
     2101 gccggttccg ggttcatcgc atggtcgcgg gccatcttcg gaatgccacg gatctggtcg
     2161 atcgcagcgg tgacctgagg ctgggcttcc ggcggaacgg gctgggtggc gatgaaggcc
     2221 ttgaccatct cggcgaaggc gtcggccttg ggcttgtagc gggcgacaat cgcgtcggcg
     2281 tcggtattgg ccttggcggt atcggttccg gcagccgtct tggcggactg aatctccgtt
     2341 gtcatctgct cgatctgcgc ctcaaaagcc gcgctggcgg cttccacggc gtcctcatcc
     2401 ggcccggcag cggcaggcgc agcaggagcg ggggccgggg tcggagctgg cgccggggca
     2461 gtctgggcga aagccggtgc tgccatcatc aaagcggcgg ccgcaacggc ggcggtggtc
     2521 agggtacgca tgggcatcgt ctccagtgaa agacctcgac cgcgcggtcg gggctacaaa
     2581 caggtcagcc gcgcgcgttg cgcttggcca gcacccgcag gcgcagggcg ttcagtttga
     2641 tgaagcctgc ggcgtcattg tggtcataag cctgaacgcc ttcctcgaag gtcaccagat
     2701 cctgatcgta caggctgtag gggctttcgc gaccgatgat cgagacgttg cccttgtaga
     2761 gcttgatcgt gaccgtgccc gcgacctttt gctgggtgta gtcgatcgcg gcctgcagca
     2821 tctcgcgctc gggcgcgaac cagaagccgt tgtagatcag ctctgcatat ttcggcatca
     2881 actggtcctt gaggtgcatc gagccgccat cgagggtgat ggactcgatg ccccggtgcg
     2941 cggccagcag gatcgtgcca cccggcgtct catagacgcc gcgcgacttc ataccgacgt
     3001 agcggttctc gaccagatcc agacgaccga cgccattggc gtggcccagt tcgttgagct
     3061 tggtcaggat ggtggccggc gacatcgcca cgccgtcgat cgcgaccggg tcgcccttct
     3121 cgaaggtcat agtgaagacg tgcgccttat cgggcgcgtc ctcgggcgcg atggtgcgct
     3181 ggtggacgaa ctcgggagcc tcgaccgccg ggtcttcgag aaccttgccc tcggacgacg
     3241 agtgcagaag gttggcgtcg acgctgaacg gcgcttcgcc gcgcttgtcc ttggcgatcg
     3301 ggatctggtt cttctcggcg aactccagca gagcctcgcg gctgcggtat tcccactcgc
     3361 gccagggcgc gatgacgcgg atgtcgggct ccagcgcata gtagccgatc tcgaaccgga
     3421 cctgatcgtt gcccttgccg gtcgcgccgt gacagacggc gtcggcgccg accatgcggg
     3481 cgatctcgat ctggcgcttg gcgatcagtg gccgggcgat ggaggtgccc agcaggtact
     3541 ggccttcata gacggtgttg gcgcggaaca tcgggaagac gaagtcgcgc acgaactcct
     3601 cgcgcaggtc gtcgacaaag atgttctccg gcttgatgcc catcttcagc gccttctcgc
     3661 gtgccgggcc gagctcttcg ccctggccca gatcggcggt gaaggtcaca acctcggcgt
     3721 tatattccgt ctgcagccat ttcaggatga tcgaggtgtc gagaccgccc gaataggcga
     3781 ggacgacctt cttgacgggc ttgtcggaag ctttggacat ggcgggacct tcgatcggga
     3841 ggcggcaggc gcagcggcac agtttgcgcg cgcttaaagg gcctgacgcc ccgtgatgca
     3901 agggcgggaa tgcggcagcc gctttctaaa gcagggcggc gatcgcttcg cgggctgctg
     3961 cgccgagctt ggcgtgcttc agggcgtagg cgacgttggc ccgcagcagg ccgacgggat
     4021 cgccgcagtc ataggtgtcg ccctcgtact ccagcgcgtg gaaatcctgc accttcatca
     4081 accgggccat ggcgtcggtc agctggatct cgccgcccgc gcccgattgc tggtcggcca
     4141 gcagcgggaa gatatccggc gtcaggatat agcggcccga gatgaacagg ttcgacggct
     4201 cggtcccgag cggcggcttc tcgaccatgc cggtcatgcg gttcagtcgc ccgtcctgac
     4261 catccagagc cacgattccg tatttgtggg tctcgccttc gggggcctgt tcgacgacga
     4321 cgatgttgcc gccggtcttt tcgtgcgcgt cgatcgcctg tttcaaggca ggcgtgtcag
     4381 ccatcatcag catgtcggcc agaatgaccg cgaacggctc gtcgccgatc agatcgcggg
     4441 cgcaatagac ggcatggccg aggcccagcg gcgccatctg acgcacaaag ctcatctcgc
     4501 cgggcttggg caggtcggcg acgaccatct cgaggatttc gttcttgccc ttggatttga
     4561 ggatcgcctc catctcgtag gcatggtcga aatagtcctc gaccgagctc tggccgcgac
     4621 cgaccacgaa gatgaaatgc tctatcccgg ccgcacgcgc ctcttcgacg atgtgcgaca
     4681 ggatcggccg gtcgaacacg ttgagcatgt tcttgggcgt ggtcttggcc gcagggagca
     4741 cgcgcgtccc gagaccggcg acaggaagca cggccttgcg gacgcgagga aaggtagacg
     4801 acatgaattt cagccccggg gagagttctg acaacccaac gaccataggc cggattgcgt
     4861 tgccagcgga agcccaagcc gagctcgccg ggccttgcta tcagcgatcg ccgctattcg
     4921 acagtgacag atttcgccaa atttcgcggc tgatcgacgt cggcgccctt caccacggcg
     4981 acgtgatagg ccagcagctg gatcggcgcc gacatcacca gcgtggcgac cagcgggtcg
     5041 ctggccgggg ccgtgaccac cacccgcgcg ccggcaggag cgtgtttggc gccctccgga
     5101 tcggtgatga agaccacctg accgccccgg gccatgacct cgctcatgtt cgacgccgac
     5161 ttctcgaaat agctgtcgta gggcgcgagg atgatgatcg gcgtctggtc gtcgaccaag
     5221 gcgatcgggc catgtttcag ctcgcctgcg gcatagccct cggcgtggat gtagctgatc
     5281 tccttgagct tcaacgcgcc ttccagcgcc agcgccgaca tcggaccgcg cccgaggaac
     5341 agcacgtcgc gggctttggc gatgtcgaag gcgatctcct tcaaggcgtc ttccagcccg
     5401 atggcttcgg cgatcaggcg cggagcctcc agcatcacct tgaccagccg ctgctcctcg
     5461 gcagcgtcga tcctgccccg cgccttggcg gctgcgacgg ccagagcgat catcacgctg
     5521 acctgtgcag tgaaggcctt ggtcgaggcc acgccgatct cggggccgca atggatcggc
     5581 cacaccacat cgacctcgcg ggccatggtc gactcctgcg cgttgacgat caccgcgctc
     5641 ttcatgcccc tggccttgca gtagcgcagg gcggcgagag tgtcggcggt ctcgcccgac
     5701 tgcgacatgg cgatcaccag cgaactggga cgcagcgccg gctggcgata gcggaactct
     5761 gacgcgatct cgacgtccac aggcaggtca gccagctgct caatcaggta tttaccgatc
     5821 atgcctgcga tataggaggt gccgcaggct acgatctgga tccgctccag cgccttgaaa
     5881 tccacatcgc cgggcatggc ggcgcgggcg gtcagggcgt cgacataggc ggcgatcgtg
     5941 cgctggcagc cctccggctg atcatggatt tccttctcca tgaagtgccg atagttgccc
     6001 ttctccatca tcaccgccga ggcgggaacg accttcacgg cacgcacgac cggcgcgccc
     6061 gaggcgtcga agatgcgggc gctgtcgtgg tcgatggcga cgaagtcacc ctcttccaga
     6121 tagatcaccc ggttggtgaa cggccccacg gccagggcgt cggagccgag gaacatctcg
     6181 ccctgcccct cgcccaccac cagcgggctg ccgcgccggg cgccgaggat cagatcgtcc
     6241 gcgccttcaa tcagcacggc cagcgcatag gcgccggtca gccggtccag cgcggctttg
     6301 aaagcctcca gcggcgcgag gcccgtggcc agttcggcgt cgatcagatg ggcgatgacc
     6361 tcggtgtctg tgtcgctttc gaacgtgcgc cccgcagccg ccaattcggc cttcagctcg
     6421 gcgaaattct cgatgatgcc gttgtgcacg agggtcacgc gcccggcggt gtgggggtgg
     6481 gcgttcttga gattgggcgc gccgtgcgtc gcccagcgga cgtggccgat gccgttctga
     6541 ccggccagag gctctgcggc gagcagagct tcgaggttct tgatcttgcc ctgggcgcgt
     6601 cgacgctcga ccttgccgcc gaccacgact gcgataccgg ccgagtcgta gccccggtat
     6661 tccagccgct tgaggctgtc gatcagtcga tccgagacgg gcgccttgcc gacgataccg
     6721 atgatgccgc acatgctagt cgc
//
LOCUS       DASVTM010000048        12821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_164475,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000048 DASVTM010000000
VERSION     DASVTM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12821
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164475"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /gene="parC"
                     /locus_tag="VF633_01205"
     CDS             complement(<1..1894)
                     /gene="parC"
                     /locus_tag="VF633_01205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX8469704.1"
                     /translation="MTIHTPPPPEGGRIIDEPMETALSRRYLAYALSTITNRALPDVR
                     DGFKPVHRRIMYAMHQMRLNPAAAARKCAKVVGEVMGTYHPHGDASIYDALVRLAQDF
                     AQRYPLVDGQGNFGNIDGDNAAAMRYTECKLTAAAVLLMDGIDQDSVDFRATYDDQGE
                     EPTVLPAGFPNLLANGSSGIAVGMATSIPPHNVGELIDACQLLLDRPDATTAELAEIV
                     PGPDFPTGGVAVEGHASILDAYETGRGGVRLRARWERVPLERGGYQIVITEMPYQVQK
                     SKLVEALAELIELKKAPLLGDVRDESAEDVRLVIEPKSRTIDADVLMESLFKLSDLEV
                     RFPINMNVLDATGTPGVMGLKACLQAFLDHRRDVLIRRSQWRIDRVEKRLHLLEGLRI
                     VFLNLDEIIRIVREEEQPKAVFITRFGLTEMQADYILDTRLRQLARIEEMAIENEYKA
                     LAEELAMLQSLSTTPAKQWKKIGQDLAATRKALVSPRRTTIAEAVDSSAFAAPEAYIP
                     REAITVILSERGWIRAAKGKVEDPSELKFKEGDALAYLVPAFTTDRILVAASDGRVFT
                     IGADKLASGRGHGEPLRLMIELEEKADIVAVMAHAPGQKVLVASKDGYGFVAPEDDLT
                     AQKRGGK"
     gene            complement(2377..3108)
                     /gene="recO"
                     /locus_tag="VF633_01210"
     CDS             complement(2377..3108)
                     /gene="recO"
                     /locus_tag="VF633_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX8469705.1"
                     /translation="MEFHEEAFVLSARAHGDTGVVVDLLTESHGRQTAYVAGGASRKM
                     KPFLQAGARVLADYRARTSDNLGSARLEAVGEGPSALFEDSMALTGLAAATAVTQGAL
                     PEREAHPGAFLAFEALMSAFMIPEVWPAIFVRFEAGLLEDLGFGLDLSRCAATGSMDD
                     LIYVSPRTGRAVSREAGAPYADKMLKLPPFMLGAQAGLGEGDVGSGFDLTGHFLEQFV
                     FHPQNKPLPPARDWMVAKLRDLGRL"
     gene            complement(3108..3599)
                     /locus_tag="VF633_01215"
     CDS             complement(3108..3599)
                     /locus_tag="VF633_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8469706.1"
                     /translation="MTIVAIALIAALQTAPSDAAEASIGAVLDQLNVASTAADAPAYF
                     SLFTPDARYIGTDRTERWSMAEFRSYVEPYFAQGKGWTYTPSDRVITVADIDCRCVAW
                     FDEQLSNTSYGATRGSGVLRLTPKGWKIEQYVLSFAVPNDAAKAVVAVIAAPPAPTPA
                     TAH"
     gene            3783..4136
                     /locus_tag="VF633_01220"
     CDS             3783..4136
                     /locus_tag="VF633_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8469707.1"
                     /translation="MQPTPDPLDAKFAALADPTRRAILARLTEGEASVNELAAPFDMS
                     LPAVSKHLKVLEKAGLISRGREAQWRPARLEPMALKGVFDWLEHYRRFWDKSFDRLDA
                     YLKQIQKGDPDGPKN"
     gene            4120..4632
                     /locus_tag="VF633_01225"
     CDS             4120..4632
                     /locus_tag="VF633_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8469708.1"
                     /translation="MDQRTDIATEAADPCLATGAHGHALVLERILDATPEKIRRACLT
                     PEILAQWFCPKPWYITDAVIEPEVGGRFAFTMHGPNGEVMPNDGVFLLVEPHRWITTD
                     AFVKGWLPAGQPFMAATIELTPLPDGKTRYVATARHWSEATMKQHEQMGFHEGWGTVA
                     DQLAELLKTL"
     gene            4656..5138
                     /locus_tag="VF633_01230"
     CDS             4656..5138
                     /locus_tag="VF633_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8469709.1"
                     /translation="MALLQKHPSETRPTLKMQRVFAAPRELVWMAWSQPEITVQWMGP
                     VEWPAVSATSDFRVDGAWRVCLRSPDTGEDLWQGGVYREIVPPERLVFTFKWEESHED
                     GPPVDTLVTVELHETTDGKTRMDFTHEGLKSEQSLTGHKHGWNSTADRLEAWLAANPD
                     "
     gene            5193..5636
                     /locus_tag="VF633_01235"
     CDS             5193..5636
                     /locus_tag="VF633_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8469710.1"
                     /translation="MKLVTSLSFSGECRQAFEFYQSVLGGEIKAFPFGDGPPDMPVDP
                     KYRDWLMHGWLQVGDQAIMGADMPPEFAPNIDKPKNGFDVTFHTDDPAEAKRVYDGLS
                     AGGKPGMAFGETFWSPGYGSFTDKFGIPWMINTNPSADWNPGGAA"
     gene            5636..6049
                     /locus_tag="VF633_01240"
     CDS             5636..6049
                     /locus_tag="VF633_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8469711.1"
                     /translation="MKASPAVWIGRVLTGLFALFMLGASIMPKFFMPEIVAQSMIPNG
                     WTGDKTLLLGIIEFSCLALYLIPRTAVFGAVLMTGYLGGAIATNLRVDNPLFGFTLFS
                     VYLGLFMWGGLWLRDPVLRTRFPLRRAPTISGASL"
     gene            6046..6669
                     /locus_tag="VF633_01245"
     CDS             6046..6669
                     /locus_tag="VF633_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8469712.1"
                     /translation="MRKLIAAAFVSLDGVMQAPGGPGEDPSGGFAFGGWTAPFWTGDS
                     SPFMGVFEQSSDHPFDLLLGRRTYDIFAAYWPVHQDHPIGPLFDRVTKYVATSTPDSL
                     EWMNSVGLSGDVPAEIARLKRGDGPELLTQGSSVLLHTLLAHDLIDELRLMTFPVVLG
                     EGKRWFDTGSRPAGFNLERSETSDGGVMSSVYRRGGEVRTGTVGAAD"
     gene            complement(6679..6912)
                     /locus_tag="VF633_01250"
     CDS             complement(6679..6912)
                     /locus_tag="VF633_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469713.1"
                     /translation="MPRQALQPGSPGVHTQRDTSELPSDLEYPVREYIGGMTRELARM
                     AREDGDEVLAVQLERASERAALSIQIRPVARIA"
     gene            7067..9745
                     /gene="topA"
                     /locus_tag="VF633_01255"
     CDS             7067..9745
                     /gene="topA"
                     /locus_tag="VF633_01255"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX8469714.1"
                     /translation="MKLVIVESPAKAKTINKYLGSDFEVLASYGHIRDLPSKDGSVKP
                     DEDFAMEWEVDARGAKRMTEIAEAAKRADRIILATDPDREGEAISWHLLEVLNKKKAL
                     KDKHVERVTFNAITKSAVLEAMAAPRQIDMELVEAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRIVVDRELEIERFKALEYWSVEADLAADSPPFTTRLVKHDGKRI
                     QRLDIPSEAVAFAARDAIKSGDFTIKSIEKKPVRRFPSPPFTTSTLQQEASRKLGFNA
                     QRTMQAAQKLYEGVDEQGGLITYMRTDGVQTTPEGIAQARSVISDTFGPAFVPAEPRY
                     YKTKAKNAQEAHEAIRPTNIARHPDSLRLDGDLQRLYELIWKRMVASQMEAARIDRTT
                     VDIETPDGQTGLRATGQVVTFDGFLAVYEEGRDEKQKGAETDEDDDSTRLPSLKEGAK
                     AKVEAIRTEQHFTEPPPRFSEATLVKKLEELGIGRPSTYASILTTLRDREYVRMDKNR
                     FIPEDNGRLVTAFLEQFFRQWVEYDFTAGLENQLDEVSAGNADWKALLREFWSKLKPA
                     TAAMLERQGVIDELDEAIGPFLFPPKEDGSDPRTCPVCGTGRLHLKASFKMRSSFIGC
                     SNYPECRYTRGFGAGAGSAEAEAGGDRELGIDPETGQPVSLKIGRFGPYVETANPDAD
                     AAKPKRSSLPKGWSPASLTLEQALRLLALPREVGMHPEDGKPITAGLGRYGPFVLHAG
                     TYANVADIDEVFEVGLNRAVVLLAEKRAGKFAGRGAAVAPLKDLGAHPETGEPIHVMA
                     GRFGPYVKSGKINATLPKGTAPEDLTLEVALPLLAARALAAPAKGKKAPAKKAAAPKA
                     AASKTTAAKKPAAKKAPAKKAAAKKPAAKKVAPVEE"
     gene            9988..11700
                     /gene="leuA"
                     /locus_tag="VF633_01260"
     CDS             9988..11700
                     /gene="leuA"
                     /locus_tag="VF633_01260"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX8469715.1"
                     /translation="MLRDPSVKYRPFPQVDIPDRTWPSKTITKAPRWLSTDMRDGNQA
                     LIDPMNAEKKQRFFDLLVKIGIKEIEVGFPAAGATEFDFIRSLVDRGAIPDDVMPQVL
                     TQARADLIDKTFDSLDGAKQAVVHVYNAVSPAWRRIVFGMERPEIKGIAETAAKLLRD
                     QAARRPNTDWRFEYSPETFSTAELDFSIECCEAVMDILQPTPDRPLILNLPATVEAAS
                     PNVYADQIEWFGRNISRRDSVIISVHPHNDRGTGVAAAELSMMAGADRVEGCLFGNGE
                     RTGNVDLVTLALNLYTQGVDPELDFSDISEVVRTVEYCNQLPVHPRHPYAGELVFTAF
                     SGSHQDAIKKGFAAQAARNDQMWDVPYLPIDPADIGCSYEAVIRVNSQSGKGGVAWIL
                     EQDKGLKLPKRLQADFSRHVQELADKTSRELNAADIWGAFQIAYHLDANQPLRLIDYQ
                     EGAVTGGERHFVGRISLDDQEHTVSGRGNGLLSSVLDGLREGVGIDLEIADYQEHAIG
                     HGSDVQAAAYVECKGADGSTVFGVGIDADVATASVKALLSAATTARAAAKARNAKAPE
                     PATV"
     gene            complement(11697..>12821)
                     /locus_tag="VF633_01265"
     CDS             complement(11697..>12821)
                     /locus_tag="VF633_01265"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX8469716.1"
                     /translation="AGQVSPGYSSPWAAPSIPRKAPRWLMMKHAPLILRPAVDWEMIS
                     WGVAMLRNCTSTRYALNKERMVRLAEYSRDQLDALRGETGITYDGRQQGTLQLFRTEK
                     QLHDVGKDVKVLNAEGVPCEVLDPAGCIAAEPGLGRSEATFVGGLRLPHDQTGDCSLF
                     TIRLAEMAAAAGVVFRFNETVQRVNVDAGRIASVTTDKGDLRADAYVLALGSYSAAVA
                     RSIDLRLPVYPVKGYSISMPIVDKTAAPVSTVMDESYKVAITRLGDRIRVGGMAELSG
                     FSTDLPPARERTLKHSVSTLFPGAGDLDAAHFWSGLRPMTPDGTPVVGPSRISNLHIN
                     TGHGTLGWTMACGSARVLADQMSGLKPEIETADLSIARYG"
BASE COUNT         2371 a         4297 c         4034 g         2119 t
ORIGIN      
        1 gtttgccacc acgcttctgg gcggtcaggt cgtcctcagg cgccacgaag ccatagccgt
       61 ctttggacgc gaccagcact ttctggcccg gcgcatgggc catgacggcg acgatgtccg
      121 ccttttcctc cagttcgatc atcagccgca gcggctcgcc atggccgcga ccggaggcca
      181 gcttgtccgc gccgatcgtg aacacccgac cgtccgacgc cgccaccagg atcctgtcgg
      241 tggtgaaggc ggggacgagg taggcgagcg cgtcgccctc cttgaacttc agctccgacg
      301 ggtcctcgac cttgcccttg gcggcgcgga tccagccgcg ttccgacagg atgacggtga
      361 tggcttcgcg cgggatatag gcttcgggcg ccgcgaaggc cgagctgtcg acggcttcgg
      421 cgatcgtggt ccggcgcgga gagaccagcg ccttgcgggt cgcagccaag tcctggccga
      481 tcttcttcca ctgcttggcc ggcgtggtgg acagggactg gagcatggcc agttcctcgg
      541 ccagcgcctt gtactcgttc tcgatcgcca tctcctcgat ccgcgccagc tgacgcagac
      601 gggtgtcgag gatgtagtcg gcctgcatct cggtcaggcc gaagcgggtg atgaagacgg
      661 ccttgggctg ttcctcctcc cgcacgatgc ggatgatctc gtcgaggttc aggaagacga
      721 tcctgagacc ttccaacaga tgtagtcgct tctcgacgcg atcgatccgc cattgcgagc
      781 gccggatcag cacgtcgcgg cggtgatcga ggaacgcctg cagacaggcc ttcagtccca
      841 tcacgccggg cgtgccggtg gcgtccagca cgttcatatt gatcggaaag cggacctcca
      901 gatcggacag cttgaacagg ctctccatca ggacgtcggc gtcgatggtg cgcgacttgg
      961 gctcgatgac cagccgcacg tcctcggccg actcgtcgcg gacatcgccc agcaacggcg
     1021 ccttcttgag ctcgatcagt tcggccagag cctcgaccag cttggacttc tggacctgat
     1081 acggcatctc ggtgatgacg atctggtagc cgccgcgctc gagcggaacc cgctcccagc
     1141 gtgcgcgcaa ccgcaccccg ccccggccgg tctcataggc gtccaagatg gaggcatgtc
     1201 cttcgacggc gacgccgccg gtcgggaagt cggggcccgg cacgatctcg gccagttcgg
     1261 cggtggtggc gtcaggccgg tccaacagca actggcaggc gtcgatcagc tcaccgacgt
     1321 tgtgcggcgg gatcgacgtc gccatgccca cggcgatgcc cgacgagccg ttggccagca
     1381 ggttggggaa gccggccgga agcacggtcg gctcctcgcc ctgatcgtca taggtggcgc
     1441 ggaaatcgac cgagtcctga tcgatgccgt ccatcagcaa gacggcggcg gcggtcagct
     1501 tgcactcggt gtatcgcatg gctgcggcgt tatcgccgtc gatgttgccg aagttcccct
     1561 ggccgtcgac cagcggatag cgttgcgcaa agtcctgcgc cagacggacc aacgcgtcat
     1621 agatcgaggc gtccccgtgc gggtgatagg tgcccatcac ctcgcccacg accttggcgc
     1681 acttccgcgc cgccgccgcc gggttcagcc gcatctgatg catcgcgtac atgatgcggc
     1741 ggtgcaccgg cttgaagccg tcgcgcacgt ccggcagcgc ccggttggtg atcgtggaca
     1801 gggcataggc caggtaacgg cggctcagcg ccgtctccat gggctcgtcg attatacggc
     1861 cgccctcggg aggcggagga gtgtggatgg tcatgcggga tcgaatcagc cggtttggga
     1921 cggaagtcta gtgcggggaa tggtcaaggt gcagagccgg atcgctatcc gcctcgaaag
     1981 ccgccgggct cgtgctttcg aggccaccgg tccagcggcc gccctaaagt cggaaaaacg
     2041 acttatgcaa tggaaccggt agaaggagga actgttctat ctgcgaacca gcgttattgg
     2101 acgcgaaatc tcagatgaag acgcctacac agccagatca gttcgaccat caggttttcg
     2161 acctttcgtt catcaactcg gtgatcaaag acattcacgg aagacttgcc acaccgatcg
     2221 gggggtttcg taagtcccac gccatggtgt tcaggtaacg acaacatcgc gagtgtgaca
     2281 gcggctgaag agcgcgttgc tcacgtcgat ggcattctcg acttgccagc cgagtgagaa
     2341 cgccccgtac gcagatcacc ctcggttgat caggcgctat aaccgcccca gatcccgcaa
     2401 tttggccacc atccagtcac gcgcgggcgg caatggcttg ttctgcgggt ggaagacgaa
     2461 ctgttcgagg aagtgaccgg tcaggtcgaa gcctgagccg acgtcgccct cgccgagccc
     2521 cgcctgggcg ccgagcatga atggcggcaa cttcagcatc ttgtcggcat agggagcgcc
     2581 ggcctcgcgg ctgacggcgc gaccggtgcg ggggctgaca tagatgaggt cgtccatgct
     2641 tccggttgcg gcgcagcgcg acaggtcgag gccaaagccg aggtcttcca gcaaccccgc
     2701 ctcgaagcgc acgaagatcg cgggccagac ctcggggatc ataaacgccg acatcagggc
     2761 ctcgaatgcc aggaaggcgc cgggatgggc ctcacgttcg ggcagcgctc cttgcgtcac
     2821 cgcagtcgca gcggcgagac cggtcagggc catcgagtcc tcaaacaggg cgctgggccc
     2881 ctcccccacc gcttccagcc gcgccgatcc cagattgtcc gacgtccgag cgcggtagtc
     2941 ggcgaggacc ctcgcgccgg cctgcaggaa cggcttcatc ttgcgtgatg ccccgcccgc
     3001 gacataggcc gtttgacggc cgtggctctc ggtcaggagg tcgacgacga cgccggtatc
     3061 gccatgcgcc cgggccgaca ggacgaaagc ttcctcgtgg aactccatca gtgggcggtc
     3121 gccggtgtcg gggctggcgg agcggcgatc accgcgacca ccgccttggc cgcatcgttc
     3181 ggcacggcga agctgaggac atactgttcg atcttccagc ccttaggcgt caagcgcagc
     3241 accccggagc ctcgcgtcgc gccgtaggac gtgttgctga gttgctcatc gaaccaggcc
     3301 acacagcggc agtcgatgtc ggcgacggtg atgacgcggt ctgacggcgt gtaggtccag
     3361 cctttgccct gagcgaaata cggttcgacg tacgaccgga actccgccat cgaccagcgc
     3421 tcggtccggt ccgtgccgat gtaacgtgcg tccggcgtga acaggctgaa ataggcgggc
     3481 gcatcggccg cagtcgaagc gacgttcaac tgatccagca ccgcgccgat gctggcttcc
     3541 gccgcgtccg aaggagcggt ctgaagcgct gcgatgagcg caatggcgac aatggtcatg
     3601 acgacagttc ccgttttgta cacctctcat agcggcgaca ggcccaacgg caaatcgcta
     3661 aatcgccatg acctcatacc gagcctgagc gccaaggccc caccaaacac ctatttaacc
     3721 catcggttga caaccgcttc gcccggccgt atttaactga taggttagat tgaagacaca
     3781 tgatgcagcc cacccccgat cctcttgacg ccaagttcgc cgcgctggcc gacccgaccc
     3841 gtcgggccat tctggcgcgg ctgactgagg gcgaggcgag cgtcaatgaa ctggcggcgc
     3901 cgttcgacat gagccttccg gcggtctcaa aacatctgaa ggtgctggaa aaagccgggc
     3961 tgatctcacg cggtcgcgag gcccagtggc gccccgcacg gctggagccg atggcgctga
     4021 agggggtctt cgactggctg gaacactaca ggcgtttctg ggacaaatcg ttcgatcgtc
     4081 tcgacgccta tctgaaacaa atacagaagg gagaccccga tggaccaaag aactgacatc
     4141 gcgaccgagg ccgccgatcc ctgcctcgcc accggcgctc acggccacgc cctggtgctg
     4201 gaacgcatac tggacgccac gccggagaag atccgcagag cctgcctgac gccggaaatc
     4261 ctggcccagt ggttctgtcc caagccctgg tacatcaccg acgcggtcat cgagcccgag
     4321 gtcggcggcc gcttcgcatt caccatgcat gggccgaacg gcgaggtcat gcccaatgac
     4381 ggcgtcttcc tgctggtcga gccacaccgc tggatcacca ccgacgcctt cgtgaagggc
     4441 tggctgcccg caggccagcc gttcatggcc gcgacgatcg agctgacccc cctgcccgac
     4501 ggcaagaccc gctatgtcgc gactgcccgc cactggtcag aggcgacgat gaagcaacac
     4561 gaacaaatgg gcttccacga aggctgggga accgttgccg atcagctggc cgaactgctg
     4621 aagaccctct gatccaacgc tagcggaaac ccaacatggc cttgctgcag aaacaccctt
     4681 ccgaaacccg cccgacgttg aagatgcaac gcgtcttcgc cgccccgcgc gagctggtct
     4741 ggatggcctg gagccagccc gagatcacgg ttcaatggat gggaccggtc gaatggccgg
     4801 ccgtcagcgc gacctccgat ttccgcgtcg acggcgcgtg gcgcgtctgc ctgcgttcac
     4861 ccgacaccgg cgaggatctg tggcagggcg gcgtgtaccg cgagatcgta ccgcccgaac
     4921 ggctcgtctt cacgttcaag tgggaggaga gccacgagga cggccctccg gtcgataccc
     4981 tggtcaccgt cgaactgcac gagacgaccg acggcaagac ccggatggac ttcacccacg
     5041 agggcctgaa gtccgaacag agcctgaccg gccacaaaca cggctggaac tctacggccg
     5101 acaggctcga agcttggttg gccgccaatc ccgactaacc gccggctact ggctgaccca
     5161 ccaaacccac aacaacaaga ggagacgccg tcatgaaact ggtcaccagc ctgagcttct
     5221 ctggcgaatg ccgccaggcc ttcgaattct atcagtccgt actcggcggg gagatcaagg
     5281 ccttcccgtt cggcgacggc ccgcccgaca tgccggtcga tccgaaatac agggactggc
     5341 tgatgcacgg ctggctgcag gtcggcgatc aggcgatcat gggcgccgac atgccgccgg
     5401 agttcgcgcc taacatcgac aagcccaaga acggcttcga cgtcaccttc cacaccgatg
     5461 atccggccga ggcgaaacga gtctatgacg gcctgtctgc gggcggcaag ccgggcatgg
     5521 ccttcggcga gaccttctgg tcgccgggct acggctcctt caccgacaag ttcggcattc
     5581 cgtggatgat caacaccaat ccgtcggcag actggaaccc ggggggagcc gcctgatgaa
     5641 ggcgtccccc gccgtctgga tcggccgcgt cctgaccggc ctgttcgccc tgttcatgct
     5701 gggcgcgtcg atcatgccca aattcttcat gccggagatc gtcgcacaaa gcatgatccc
     5761 caacggctgg acgggtgaca agaccctgct gctcgggatc atagagttca gttgcctcgc
     5821 cctctatctg atcccgcgca ccgccgtctt cggcgcggtg ctgatgaccg gctacctggg
     5881 cggagctatc gccaccaacc tgcgggtcga caacccgctg ttcggcttca ccctgttcag
     5941 cgtctacctc ggcctgttca tgtggggtgg gctgtggctg cgcgatccgg ttttgagaac
     6001 ccggttcccg ttgcgccgag cacccacgat ctcgggagca tcattatgag gaagctgatc
     6061 gctgccgcct tcgtcagtct ggacggcgtc atgcaggcgc cgggcgggcc tggggaagat
     6121 ccctccggcg gtttcgcctt tggcggctgg accgcgccat tctggaccgg cgactccagc
     6181 ccgttcatgg gcgtgttcga gcagtcatcg gaccacccgt tcgacctgtt gctgggccgc
     6241 aggacctacg acatcttcgc cgcctactgg cctgtgcatc aggatcatcc gattggaccg
     6301 ctgttcgacc gtgtgaccaa atatgtcgcg acctcgacgc ccgacagcct ggaatggatg
     6361 aactccgtcg gcctgtccgg cgacgttccc gcagagatcg cccggttgaa acgaggcgat
     6421 gggccagagc tgctgacgca agggtccagc gtcctgctcc acaccctgct cgcgcatgat
     6481 ctgatcgacg aactgcgcct gatgaccttc ccggttgtgc tcggcgaggg caagcgctgg
     6541 ttcgacacag gatcgcggcc cgccggcttc aaccttgagc ggagcgagac ctccgacggc
     6601 ggcgtcatgt cgagtgtcta ccgccggggc ggtgaagtca ggaccgggac ggtgggcgca
     6661 gcggactgag cctgcgtcct atgcaatgcg agccacaggc ctgatctgaa tcgacagggc
     6721 ggcgcgctct gacgcgcgct caagctggac agccagcact tcatcaccgt cctcgcgggc
     6781 catccgggcg agctccctcg tcatgcctcc gatatattcg cggaccggat attccaggtc
     6841 cgacgggagt tccgaggtat cccgttgcgt atggacgccc ggcgatccgg gctgaagagc
     6901 ttggcgcggc atgcaaaaac ctccgaaaca aacaaccaac tcactggaac acaacttcaa
     6961 gtaacattgc aacccttacg ggaagttgtg atgcaggaag ctccactgat tgacacggcc
     7021 cccggcgcca accacgttcg ccgcctccca aacagactgc gcagtaatga aactcgtcat
     7081 cgtcgagagc ccggccaagg ccaagaccat caataagtac ctcggatccg acttcgaggt
     7141 gctcgcctct tacgggcaca tccgcgacct tccgtcgaag gacggatcgg tcaagccgga
     7201 cgaggacttc gccatggaat gggaagtcga cgcccgcggc gccaagcgca tgaccgagat
     7261 cgccgaggcg gccaaacgcg ccgaccgcat cattctcgcc accgaccccg accgtgaggg
     7321 agaggcgatc agctggcacc tgctcgaggt tctgaacaag aagaaggcgc tcaaggacaa
     7381 gcacgtcgag cgcgtgacct tcaacgccat caccaagtcc gccgtgctcg aagccatggc
     7441 cgccccgcgc cagatcgaca tggaattggt cgaggcctat ctggcccgcc gtgcgctcga
     7501 ttatctggtg ggcttcaccc tgtcgccggt gctgtggcgc aagctgcccg gcgcccggtc
     7561 ggccggtcgg gtgcagtcgg tcgccctgcg tatcgtcgtc gatcgcgagc tcgagatcga
     7621 acgcttcaag gcgctggaat actggtcggt cgaggccgat ctggccgccg acagcccgcc
     7681 gttcaccacc cgtctggtca agcacgacgg caagcggatc cagcgtttgg atatcccgtc
     7741 cgaggccgtg gccttcgccg ctcgtgacgc gatcaagtcg ggcgacttca ccatcaagtc
     7801 gatcgagaag aagccggttc gccgcttccc ctccccgccc ttcaccacct cgaccctgca
     7861 gcaggaggcc tcacgcaagc tcggcttcaa cgcccagcgc accatgcagg cggcgcagaa
     7921 gctgtacgaa ggcgtcgacg agcaaggcgg cctgatcacc tatatgcgga ccgacggcgt
     7981 ccagacgacg cccgagggca tcgctcaggc tcgctcggtc atcagcgaca cgttcggccc
     8041 ggcctttgtc ccggccgagc cgcgctacta caagaccaag gccaagaacg ctcaggaagc
     8101 gcacgaggcg atccgcccga ccaacatcgc ccgccatccc gactcgcttc gtctcgatgg
     8161 cgacctgcaa cggctctatg agctgatctg gaagcgtatg gtcgcctcgc agatggaagc
     8221 cgcccgcatc gaccgcacca cggtcgatat cgagacgccc gacggccaga ccggcctgcg
     8281 cgccactggc caggtcgtga cctttgatgg cttcctcgcc gtctatgaag aaggccgcga
     8341 cgaaaagcag aagggcgccg agaccgacga ggacgatgat tcgacccgcc tgccgtcgct
     8401 caaggaaggc gcgaaggcca aggtcgaggc catccgcacc gaacagcatt tcaccgagcc
     8461 gccgccgcgc ttctcggaag ccacgctggt caagaagctc gaagagctcg gcattggccg
     8521 tccctcgacc tacgcctcga tcctgaccac cctgcgcgac cgcgaatacg tccgcatgga
     8581 caagaatagg ttcatccccg aggacaacgg gcgtctggtg acggccttcc tcgaacagtt
     8641 cttccgccag tgggtcgaat acgacttcac cgccggactg gagaaccagc tcgacgaggt
     8701 atccgcaggc aatgccgact ggaaggcgct gttgcgtgag ttctggtcca agctgaagcc
     8761 ggcgaccgcc gccatgctgg agcgtcaggg cgtcatcgac gaactggacg aggccatcgg
     8821 gcccttcctg ttcccgccca aggaagacgg atcagacccg cgcacctgtc cggtgtgtgg
     8881 cacgggacgt ctgcacctga aggccagctt caagatgcgg tcgtccttca tcggctgctc
     8941 caactatccc gaatgccgct acacgcgcgg cttcggcgcc ggtgcaggct cggcggaagc
     9001 cgaggccggt ggcgaccgcg aactgggtat cgatcccgaa accggccagc ccgtctcgct
     9061 gaagatcggc cgctttggtc cctatgtcga aacggccaac cccgacgccg acgccgccaa
     9121 gccgaaacgc tcctccctgc ccaagggctg gagcccggcg tcgctgacgc tggaacaggc
     9181 gctgcggctg ctggccctgc cgcgcgaggt cgggatgcac ccggaggacg gcaagccgat
     9241 cacggcgggc ctcggccgtt acgggccgtt cgtcctgcac gccgggacct acgccaacgt
     9301 cgccgatatc gacgaggtgt tcgaagtcgg attgaaccgc gcggtcgtgc ttctggccga
     9361 gaagcgtgcg ggcaagttcg cgggtcgtgg cgcggccgtg gctccgctca aggacctcgg
     9421 cgcccacccg gaaacgggcg agccgatcca cgtgatggcc ggtcgcttcg gcccctatgt
     9481 gaagtcgggc aagatcaacg ccaccctgcc caagggcacg gcccccgagg acctgacgct
     9541 ggaagtcgcc ctgccgctgc tggccgcccg cgccctcgcc gcccccgcca agggcaagaa
     9601 ggctcccgcc aagaaggcgg ctgctccaaa ggctgcggcc tccaagacga ccgcagccaa
     9661 gaagcctgct gcgaaaaagg ctccggccaa gaaagctgct gcgaagaagc ccgcagccaa
     9721 gaaagtcgcg cctgtcgaag agtgattcct ctcccttcgg ggagaggaac tttcgcctcg
     9781 cacgcatttt ttgaccgtta gggcgagcca aggcgatgat tcttcgctat agggttcgga
     9841 tggtctgtca ctcgacgcaa tatcgccgcc tgccgctccg gctcgacgcg cgacttatcg
     9901 gcggccgccg cgcctgatct gcgcccggcg gctttgactg cgtcgtaact tctacagacc
     9961 ctccagactg aaaaaggaga cgcgccgatg ctgcgcgatc cgtccgtaaa ataccgtccc
    10021 ttcccgcagg tcgacattcc cgaccgcacc tggccgtcca agacgatcac gaaggcaccg
    10081 cgctggctgt cgactgacat gcgcgacggc aatcaggccc tgatcgatcc gatgaacgcc
    10141 gagaagaagc agcgcttctt cgacctgctg gtgaagatag gcatcaagga aatcgaggtc
    10201 ggtttcccgg ccgccggcgc caccgagttc gacttcatcc gctccctggt cgatcgcggc
    10261 gccattcccg acgacgtcat gcctcaggtc ctgacgcagg cccgcgccga cctgatcgac
    10321 aagaccttcg acagcctcga cggcgccaaa caggcggtcg tccacgtcta caacgccgtc
    10381 tctccggcct ggcgtcggat cgtgttcggc atggagcggc ccgagatcaa gggcatcgcc
    10441 gagaccgccg ccaagctgtt gcgcgatcag gccgcacgcc gtccgaacac cgactggcgc
    10501 ttcgaataca gccccgagac cttctccacc gccgagctcg atttctccat cgagtgttgc
    10561 gaggcggtga tggacatcct gcagccgacg cccgacaggc cgctgatcct gaacctgccg
    10621 gcgacggtcg aggcggcgtc gccgaacgtc tatgccgacc agatcgaatg gttcggccgc
    10681 aacatctcgc gccgcgacag cgtcatcatc tcggtccacc cgcacaacga ccggggcacc
    10741 ggcgtcgccg ccgcagagct gtcgatgatg gccggcgccg atcgcgtcga gggctgtctg
    10801 ttcggcaacg gcgagcggac cggcaacgtc gatctcgtga cgctggcgct gaatctgtac
    10861 acccagggcg tcgatccgga gctggacttc tcggacatca gcgaggtcgt ccgcacggtc
    10921 gaatactgca accagttgcc ggtccatccg cgccatccct atgctggcga actggtcttc
    10981 accgccttct caggcagcca tcaagacgcg atcaagaagg gcttcgcggc ccaggcggcg
    11041 cgcaacgacc agatgtggga cgtgccctat ctgcccatcg atccggcgga catcggctgc
    11101 tcctatgagg cggtgatccg ggtcaactcc cagtcgggca agggcggagt cgcgtggatc
    11161 ctggagcagg acaagggcct gaaactgccc aagcgcctgc aggccgactt cagccgccac
    11221 gtgcaggaac tggccgacaa gaccagccgc gagctcaacg ccgccgacat ctggggcgca
    11281 ttccagatcg cctatcatct ggacgcaaac cagcctctgc gcctgatcga ctatcaggag
    11341 ggcgcggtga ccggaggcga acgccacttc gtcggccgca tcagcctcga cgatcaggaa
    11401 cacaccgtca gcggtcgcgg caacggcttg ctgtccagcg tgctcgatgg tctgcgcgaa
    11461 ggcgtcggca tcgacctcga gatagccgac tatcaggagc acgccatcgg gcacggttcg
    11521 gatgttcagg ccgccgccta cgtcgagtgc aagggcgcgg acggctcgac cgtgttcggg
    11581 gtgggaatag acgccgacgt ggctacggcc tcggtcaagg cgctgctctc ggccgcgacc
    11641 acggcccggg cagccgccaa ggcgcgcaac gcgaaggcac cggaacccgc gacggtttag
    11701 ccgtagcgag cgatggacag atcggcggtc tcgatctctg gcttgaggcc gctcatctga
    11761 tccgccagca cccgcgccga gccgcaggcc atggtccagc ccagcgtccc gtggccggta
    11821 ttgatgtgca ggttgctgat ccggctgggc cccaccacgg gggtgccgtc cggcgtcatg
    11881 gggcgcaggc cggaccagaa gtgagcggcg tcgagatcgc ccgcaccggg aaacagcgtc
    11941 gagaccgagt gtttcagcgt gcgctcacgc gccggcggca ggtcggtgct gaagcccgat
    12001 agctcggcca ttccgccaac acggatgcga tcgcccagac gagtgatcgc caccttgtag
    12061 ctctcgtcca tcacggtcga gacgggcgca gccgtcttgt ccacaatggg catggagatg
    12121 gaatacccct tcaccggata gaccggcagc cgcaggtcga tcgaccgcgc caccgccgcc
    12181 gaatagctgc ccagcgcgag cacataggcg tcggcgcgca agtcgccttt gtcggtggtg
    12241 accgacgcga tgcggcctgc atcgacgttg acgcgctgga ctgtctcatt gaagcggaag
    12301 accaccccgg cggcagcggc catctcggcc agacggatgg tgaacaggct gcagtcgccg
    12361 gtctggtcat gcggcaggcg gagtccgccg acgaaggtcg cctccgaacg gcccaggcca
    12421 ggctcagcgg cgatgcaacc tgcaggatcc agcacctcgc acggcacgcc ctcggcgttc
    12481 agcaccttca catccttgcc gacgtcgtgc agctgtttct ccgtgcggaa tagctgcagc
    12541 gtcccttgct ggcggccgtc gtaggtgatg ccggtttcgc cacgcagggc gtcgagttga
    12601 tcgcggctgt attccgcgag cctgaccatc cgctccttgt tcagcgcata gcgggtcgag
    12661 gtgcagttcc tcagcatcgc cacgccccag gaaatcatct cccaatcgac ggcgggccgc
    12721 aggatcagcg gcgcatgctt catcatcagc caacgcggcg ccttacgcgg gatcgacgga
    12781 gcggcccagg gcgatgaata gccgggcgag acctgtccgg c
//
LOCUS       DASVTM010000049         9133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_855492,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000049 DASVTM010000000
VERSION     DASVTM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855492"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1958
                     /locus_tag="VF633_01270"
     CDS             <1..1958
                     /locus_tag="VF633_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469717.1"
                     /translation="AGSMANVQYSVNGDGTPHVALLPGSNVNYAAGGLANPHNYWIPF
                     IQGHVEDNEGEEIALRADATYDFNEGGWLDTLSVGVRYADRQQTVRYSTFNWTPIAQP
                     WNCNGPGFNADNTTPAPYPTNSGNCGGNAGHAPFQGYGAGIWESTSLGDFYDGGVYDN
                     GALVFLSRDVLRDFPRLVDALGPRTNAPLNSYTPICDRAEATEGCFTPGEILDVRERT
                     EAVYAMLRFGGDDKMLGGLTVAGNVGLRLVRTREDSDGSVAFPANTVFNGLQPCGTPL
                     SGGAIVNPSCYLTPEIRAFANGAVILDSYKADYLNWLPSFNVRLGLDDQNFIRFAYSR
                     ALSRPDIGLLRNTLAINAPTFSVTADSPNIIFNSPTAPRTAANVAGYNFVFLANSGNA
                     SLKPLTADQFDLSFEHYVGRNTSFAAGLFYKRLNDAISFGQFNRTIANGASTQSVQIN
                     GPSNTEGDSGELLGAELSYQSFFDFLPGWLSGLGVQTNYTYVQQSGINNSNLFNASSG
                     GDVGAFGAGQQSLGGSGFVIDSHRLAGVSTHTFNVVGLYEKGPLGFRIAYNWRSRYLT
                     QNLDCCIGLPVFQKSAGFLDASLRYSINSNLELSLDATNLLDTTSRYEQQIFGDSPQT
                     PNASPVFRDSAWSRVDRRYQIGLRTKF"
     gene            2019..3551
                     /locus_tag="VF633_01275"
     CDS             2019..3551
                     /locus_tag="VF633_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8469718.1"
                     /translation="MVNQTERYRIVILGGGTAGWMTAAMLVSRLSPADYEIALVESDL
                     IGTVGVGEATLPHIKHFNDALGIDEVDFVAQTQATFKLGIEFVGWGPPGERYIHPFGA
                     FGEPWRGVEFQHQWLRNRAMGRPTLPLAAYSYAVAAAHARRFDLPDKDRSSIRSTYAY
                     AYHFDAGLYAAYLRRWAIARGVDRREGLVEGVDRDAQSGDVTGLRLQSGTTIQGDLFV
                     DCSGFRSVLVGDCQEVPWRDWSACLPCDRALAVPSARAEDFTPFTRSTAQTAGWTWRI
                     PLQHRTGNGYVYSSRFIDEAAARATLLGALDSEPLDEPRLLRFQAGRREKAWSRNCVA
                     IGLASGFLEPLESTSIFLIQAAAHDLATLMPARGDSRLDPRLSDEFNRLFEIHYERAR
                     DFLILHYAINGREGEPLWDHMRRMALPESLERRIALFRTRGALPNYELGLFSRDSWLS
                     VLVGQGLSPEGYDRLADALDPEVLDPRLADFADRIASNLSTMRSHEDFVAAYRARAGA
                     MA"
     gene            3548..5020
                     /locus_tag="VF633_01280"
     CDS             3548..5020
                     /locus_tag="VF633_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX8469719.1"
                     /translation="MIGRPTRVVVVGADAPLWLAALTIHGALHKAGVTVEAVALPSRV
                     APTEVIEARPAIEALHNRLGIREARLLAATKGAFMLGRQLDGGPTDGSSGFVSYGANG
                     ASIGQEAFFPHWLRAERAGSGVPLEAFNLVAAAARLGRRLVPDSEIAAFGRADYGYAL
                     PAKAYARSLAVLARQDGLVSHQTEGLDAERGENGRLVALRLDDGRFITADLFVDVSQE
                     AVLSEGILRSPKEDWSAWSPTTRIARALTPPMPAPLAYGRVEAHRWGWLARIPTPAAT
                     HLLAAFSEDGEPAPDLRRMGFDPASSEALVQFGRTAPWRSNCVAMGPAACRLDPIFGL
                     DFHVLQLGLIHFVSRFPTSADAACERDDYNGTLSALMEHLRDFQIAHYRTQRYQGRFW
                     DQARAGDVPPALAHRLALFAARGLLGPLEHETIPADEWQSLLIGHGLRPTSHDPVADL
                     NPPEAVNDALRGMLDFIARKVSAFPTNAALPAASHSSDRP"
     gene            5033..7048
                     /locus_tag="VF633_01285"
     CDS             5033..7048
                     /locus_tag="VF633_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEX8469720.1"
                     /translation="MLARALIFLACALPLTAHAGSRECAQSPGRLLELCVSVEAGRAT
                     WEVGRLGQPVIAPSTLGLSFAGEPAVRWTALTQARWSSVDRTWEQPWGEQRIIADRHS
                     EMTVTLSGDTPLNREVVLTVRLFDDGVGFRYSYPSIPPDESVKVVADNTQYRTVGTNR
                     AWWYPAWGQERDEYLYSETDARRITTTETPLTLKRDDGLYLSIHEAALVDFPSMLLTG
                     DGAGTLTARLMPWPDGVLAQRTGPFSTPWRTILVGETPGNLADSRIELNLNEPNRLGD
                     VSWVRPGKYVGVWWEMHLDRSTWGSGDRHGATTANVQRYMDFAAENGFDNVLVEGWNQ
                     GWDGEWIANGDAFRFAAPYPDFDIAAVAEYGKHRGVGLIGHHETAGAVTNYEAQIEEA
                     MALYERYGVGAVKTGYVRHSGDIADQNGGLQWFAGQYMVRHHLQVAEIAARHHIAINA
                     HEPVKDTGLRRTWPNMISREGARGQEFNAWGNPTNPPEHLTILPFTRLLAGPMDFTPG
                     IFDIPRGGAELTRRVQSTLATQLAQYVVIYSPIQMAADLPENYQAHPDAFQFIKDVPT
                     DWETSRTLQSEIGEYVVVARLQRGAADWYLGAITDENGRTLRQPLSFLEPGRRYEAQI
                     YADGPEADYRTNPMSYVVSRQVVTSTDILPIVMRPGGGLAVRFRALP"
     gene            7185..7769
                     /locus_tag="VF633_01290"
     CDS             7185..7769
                     /locus_tag="VF633_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8469721.1"
                     /translation="MSGAALKNDCLLFPSENVATVADCSGVAAVRDVVQRSCSRRGPS
                     SHRRRSYCVLATVTLAAGPRANILLKSVGLRFGGRSIYFAQFLLGIYGGYFGGAVGLM
                     MLAVWSLATSADLRQLTPLRTVMVAAANGAAVLCFAATGLVSWAETSIVMIGGILGGY
                     VGARLSRRLPANVLRGATLSISVVTTAVFFARAY"
     gene            8136..8342
                     /locus_tag="VF633_01295"
     CDS             8136..8342
                     /locus_tag="VF633_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469722.1"
                     /translation="MTLISAGPVGDDIYAEAVGQFSEAELVRLTVAVAAINAWNRLAV
                     SFNLSDARSGAARVRNDLGSTMHG"
     gene            8411..8809
                     /locus_tag="VF633_01300"
     CDS             8411..8809
                     /locus_tag="VF633_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX8469723.1"
                     /translation="MTMQLINPPGSEPLYEAYQLSQAVRIADTVHVSGQVGYDAGGIP
                     EGKEDQIRLAFGHLKAVLACAGASMADIVSLTSYHTDPADFEGFKRVQREFIPLNYPA
                     HTAVGVSALLMPALKCEIAAIAVIGSRAVS"
     gene            8874..>9133
                     /locus_tag="VF633_01305"
     CDS             8874..>9133
                     /locus_tag="VF633_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8469724.1"
                     /translation="MPKIEIATAPTRLGSGYPQPYDQQCAGRRRWALGDAAGLSQFGV
                     NLLRLPPQQWSSQRHWHSAEDEFVYVLEGEVVLYTNAGAQVM"
BASE COUNT         1555 a         3078 c         2853 g         1647 t
ORIGIN      
        1 ctgccggctc gatggccaat gtccaataca gcgtcaacgg tgacggcacg cctcatgtgg
       61 cgctgctgcc gggctcgaat gtcaactacg cggcaggcgg ccttgccaac ccccacaact
      121 actggatccc gttcatccag ggtcacgtgg aagacaatga gggcgaggag atcgctcttc
      181 gcgccgacgc gacgtatgac ttcaacgagg gcggctggct cgacaccctc agcgtgggcg
      241 ttcggtacgc cgaccgccag cagactgtgc gttattcgac cttcaactgg acgccgatcg
      301 cccagccctg gaactgcaac ggacccggtt tcaacgccga caacaccacg cctgcgccct
      361 atccgaccaa ctccggaaat tgcggcggca acgccggtca tgcgccattc cagggttatg
      421 gcgcaggcat ctgggagtcg acctccctcg gcgacttcta tgacggcggc gtctacgaca
      481 acggggcgct cgtcttcctg agccgcgatg ttctgcgcga tttcccgcgc ctcgtggatg
      541 cactcggtcc gcgcaccaat gcgccgctca acagctacac cccgatctgc gaccgcgccg
      601 aggcgacgga ggggtgcttc acgccgggag aaatcctcga cgtgcgcgaa cgcacggagg
      661 cggtctacgc catgcttcgc ttcggcggag acgacaagat gctgggcggt ctcaccgtcg
      721 ccggtaacgt gggccttcgc ctggtgcgca cccgcgaaga cagcgacggc agcgtggcct
      781 tccccgccaa tacggtgttc aacggtctac agccctgcgg cacgcccctg tcgggcggcg
      841 ccatcgtcaa tccgtcctgc tatctcacgc cggaaatccg cgccttcgcc aacggcgcgg
      901 tgatcctcga cagctataag gccgactatc tgaactggct gccgagcttc aatgtgcgcc
      961 tcggcctcga cgaccagaac ttcatccgct tcgcctattc ccgcgccctc tcgcgaccgg
     1021 acatcggcct gttacgcaac acccttgcga tcaacgcccc gacgttttcg gtcactgcgg
     1081 actcgcccaa catcatcttc aactccccga cggcgccccg gaccgccgcc aacgtcgccg
     1141 gctacaactt cgtcttcctc gcaaactcgg ggaacgcctc gctgaagccg ctgactgccg
     1201 accagttcga cctgtcgttc gaacactatg tcggtcgcaa cacctcgttc gcggccggtc
     1261 tcttctacaa gaggttgaac gacgcgatct ccttcggcca gttcaaccgg acgatcgcca
     1321 atggcgcctc gacccagtcg gttcagatca acggcccgag caataccgag ggcgacagcg
     1381 gcgaactgct cggtgcggag ctgtcctacc agagcttctt cgacttcctg cccggatggt
     1441 tgagcgggct cggagtccag accaactaca cctacgtcca acagtctggc atcaacaact
     1501 cgaacctctt caacgcgtct tcgggcggcg atgtcggcgc cttcggggcc ggccagcagt
     1561 cgcttggcgg gtccggcttc gtcatcgact cgcacaggct cgcgggcgtt tcgacgcata
     1621 ccttcaacgt ggtgggcctc tatgaaaaag gtccgctcgg gttccggatc gcttacaact
     1681 ggcgctcccg ctacctgacc cagaacctgg actgctgcat tgggcttccg gtgttccaga
     1741 agtccgcagg cttcctggat gcgtcgctgc gttactctat caacagcaat ctggagctgt
     1801 cgctcgatgc gaccaacctg ctggatacga ccagtcggta cgagcagcag atcttcggcg
     1861 actcgcccca gaccccgaac gcgagcccgg tgttcaggga ttccgcctgg agccgcgtcg
     1921 accggcgcta ccagataggc cttcggacca aattctgacg tcctcgaaac tcatcgccgc
     1981 gaccacggtc gcggcgatgg tcttttggac ggagttccat ggtcaaccag acagaacgct
     2041 atcggatcgt gatcctcggc ggcggcaccg cgggctggat gacggccgcc atgctcgtct
     2101 cccgactgag ccccgcagac tatgagatcg ccctcgtcga gtcagacctg atcggcacgg
     2161 tcggcgtggg cgaggccacg ctccctcaca tcaaacactt caacgatgcg ctggggatcg
     2221 acgaggtgga cttcgtcgcc cagacccagg cgacgttcaa gctcggcatc gagttcgtcg
     2281 gctggggccc gccgggcgag cgctacattc atcccttcgg cgccttcggc gagccttggc
     2341 gcggggttga gttccagcac cagtggctcc ggaaccgggc catgggacgc cccaccctgc
     2401 ctctcgccgc ctattcctat gcggttgcgg cggcccacgc ccggcggttc gatctgccgg
     2461 acaaagaccg gagctcaatc cgatcgacct atgcctacgc ctaccatttc gacgccggtc
     2521 tctacgcggc ctacctgcga cgctgggcta tcgcgcgcgg cgtcgaccgc agggagggtc
     2581 tggtcgaagg cgtggacagg gacgcccagt ctggtgacgt caccgggctt cgcctgcagt
     2641 cggggacgac catccagggc gatctcttcg tcgactgcag cggcttccgc agcgtgctgg
     2701 tcggcgactg ccaggaggtc ccatggcgcg actggagcgc gtgcctcccc tgcgacaggg
     2761 cgctcgcggt ccccagcgcg cgcgccgaag acttcacgcc tttcacccgg tcaaccgccc
     2821 agacggcggg atggacgtgg cgcatcccgc tccagcaccg caccgggaac ggctacgtct
     2881 attcaagccg gttcatcgat gaagccgccg cgcgcgcgac cctgctcggc gccctcgaca
     2941 gcgagccgct cgatgagccg cgtctcctgc ggtttcaggc ggggcggcgc gagaaggcct
     3001 ggagtcgcaa ctgcgtggcg atcggactgg ccagcggctt cctcgaaccg ctcgagtcga
     3061 ccagcatctt cctgatccag gcggcggctc atgacctggc gaccttgatg cccgcgcggg
     3121 gcgacagccg cctggatccc cgcctgtccg acgagttcaa ccgcctgttc gagattcatt
     3181 acgagcgcgc acgggatttt ctgatcctgc actatgcgat caacgggcgg gagggcgagc
     3241 cgctctggga ccatatgcgt cgcatggccc tgcccgagag cctggaacgc cgtatcgccc
     3301 tgttccggac gcgcggcgcc ctgccaaact atgagctggg cctgttttcg cgagacagct
     3361 ggttgtccgt tctggtcggc caaggcctat cgccggaggg gtacgatcgt ctggcggacg
     3421 ccctcgaccc ggaggtcctg gacccgcggc tggcggactt cgccgaccgg atcgcctcga
     3481 acctctcaac gatgaggtcg cacgaagact tcgtcgccgc gtatcgggct cgtgccggag
     3541 cgatggcgtg atcgggcgtc cgacccgggt cgtcgtcgtc ggcgcggacg cccccctgtg
     3601 gctcgcagca ctgacgattc acggggcgct gcacaaggcc ggcgtcacgg ttgaagcggt
     3661 ggcgctgcct tcgcgggtcg ccccgaccga agtgatcgag gccaggccgg cgatcgaggc
     3721 gcttcataac cggctcggca tccgggaagc tcggctcctg gccgcaacca aaggcgcgtt
     3781 catgctcgga cgccaactgg acggcggccc cacggacgga tcgagcgggt tcgtaagcta
     3841 tggagccaac ggggcctcca tcgggcagga ggccttcttc cctcactggc tgagggcgga
     3901 gcgcgccggt tccggcgttc cgctggaggc gttcaacctg gtcgccgccg ccgcccgtct
     3961 tggacggaga ctggttcctg acagcgagat cgcggccttc ggccgagcgg attatggata
     4021 cgccctgccc gccaaggctt acgccaggag tctggccgtg cttgcacgac aggatggcct
     4081 ggtgtcgcat cagacagaag gcctcgacgc cgaacgtggc gagaacggtc gccttgtcgc
     4141 tctccgcctc gatgacggcc ggttcatcac cgccgacctg ttcgtcgatg tcagccagga
     4201 ggccgtcctc agtgagggca tcctgcggtc ccccaaggag gactggagcg cttggtcgcc
     4261 gaccacccgc atcgcccgcg cgctgactcc gcccatgccc gcgccgctgg cctacggtcg
     4321 ggtggaggcc caccgctggg gctggcttgc ccgcatcccg acgcccgcgg cgacccatct
     4381 gttggcggct ttttcggagg acggcgagcc tgcgccggat ctgcgcagga tgggcttcga
     4441 ccccgcgtcg tccgaggcgc tagtgcagtt cggacgaacc gctccctggc gctccaactg
     4501 cgtggccatg ggcccggccg cttgccgcct ggatccgatc ttcggactcg atttccacgt
     4561 gctccagctg gggctgatcc atttcgtcag ccggttcccg acttccgccg acgccgcttg
     4621 cgaacgcgat gactacaatg ggaccctctc cgcccttatg gagcatctcc gcgatttcca
     4681 gatcgcccac tatcgcaccc agcggtacca gggccgcttc tgggatcagg cgcgcgccgg
     4741 agacgtgccg cccgcgctgg cccaccggtt ggcgctcttc gcggcgcgag ggctgctcgg
     4801 cccgctcgaa catgagacga tcccggccga cgagtggcag tcgctcctga tcggtcacgg
     4861 cttacggccg acgagccatg accccgtcgc cgacctgaat ccgcccgagg cggtcaacga
     4921 cgcccttcgg ggcatgctcg acttcatcgc ccgaaaagta tccgcctttc cgacaaacgc
     4981 ggccctgccc gctgcgtctc actcctccga ccgcccttag gatgtccaga ccatgttagc
     5041 tcgcgccctg atcttcctcg cctgcgccct gcccttgacc gcccacgccg gctcgcgtga
     5101 gtgcgcccaa tcgccgggcc ggctgctcga actgtgcgtc tcggtcgagg cgggtcgcgc
     5161 gacctgggag gtcgggcgcc tcggccagcc ggtcatcgcc ccctcgacgc tgggtctttc
     5221 gtttgcaggc gaaccggccg tccgctggac ggccttgacc caagcgcggt ggtcgtccgt
     5281 cgaccggacc tgggagcagc cctggggcga gcaacgcatc atcgcggaca gacattccga
     5341 gatgacggtg acgctcagcg gcgatacgcc gctcaaccgg gaggtcgtcc tcaccgtgcg
     5401 cctgttcgac gatggcgtgg ggttccggta cagctatccg tcgatcccgc cggacgagtc
     5461 cgtgaaggtc gtcgcggaca atacccagta ccgcacggtg gggacgaacc gggcatggtg
     5521 gtatccggct tggggtcagg aacgcgacga atatctctat tcggagaccg acgcgcgccg
     5581 gatcacgacg acggagacgc cgctcacgtt gaagcgcgat gacgggctgt atctctcgat
     5641 ccacgaggcc gcgctcgtcg acttcccgag catgctgttg acgggcgacg gcgcagggac
     5701 gttgaccgcc cggctgatgc cttggccgga tggcgttctg gctcaaagga caggcccgtt
     5761 ctccacccca tggcggacca ttctcgtcgg cgaaacaccc gggaacctgg cggatagccg
     5821 cattgaactg aatctgaacg agccgaaccg tctgggcgac gtgtcctggg tcaggccggg
     5881 gaaatacgtc ggcgtctggt gggagatgca cctggaccga agcacctggg gcagcggcga
     5941 tcgtcatggc gccaccaccg ccaatgtgca gcgctacatg gacttcgcgg cggagaacgg
     6001 tttcgacaac gtgctggtgg agggatggaa ccagggttgg gacggagagt ggatcgccaa
     6061 cggggacgcg ttccggttcg cggcccccta ccccgacttc gacatcgcgg cggtcgccga
     6121 gtacggcaag catcgcggcg taggcctgat cggccaccac gagacggcgg gcgcggtgac
     6181 aaactatgag gctcagatcg aggaggccat ggcactctac gagcgctatg gcgttggcgc
     6241 ggtgaagacc ggctatgtgc ggcacagcgg cgacatcgca gaccagaatg gcggcctgca
     6301 atggtttgcc ggacaatata tggttcgcca tcatctgcag gtggccgaga tcgcggcgcg
     6361 tcaccacatc gcgatcaacg cccacgagcc ggtgaaggac accggcctgc gccgaacctg
     6421 gcccaatatg atcagccggg aaggcgctcg gggccaggag ttcaatgcct ggggcaatcc
     6481 cacgaacccg cccgaacatc tgaccatcct gccattcacc cggctgctcg ccggaccgat
     6541 ggacttcacc cccgggattt tcgacatacc gcgcggcggg gccgaactga cccgcagggt
     6601 tcagtcaacc cttgcgaccc agttagccca gtacgtcgtc atctacagtc ccatccagat
     6661 ggccgccgac ctgccggaaa actaccaggc tcaccctgac gcattccagt tcatcaagga
     6721 cgtgccgacg gactgggaga cgagccggac gctccagtca gagatcggag agtacgtcgt
     6781 ggtcgcccga ctacaacggg gcgccgccga ctggtatcta ggggcgatca cggacgaaaa
     6841 cgggcgcaca ctccgtcagc cgctcagctt tctggagccc gggcgcaggt acgaggcgca
     6901 gatctacgcg gatgggcctg aggccgacta tcgcaccaac ccgatgagct atgtcgtgtc
     6961 ccgccaggtc gttacgagca ccgacattct tcctattgtc atgcgtccag gaggaggcct
     7021 ggcggtgcgg ttccgcgcgc tgccatgacg gagagggtcc aaacttccgg acgcggcgat
     7081 cgtggccctc gccaaatcac ctcggtttgc gtctctggac gcgtgcaacc gacacgggcc
     7141 ggaaacctcc ctcgcgccat catatatcgg caacgagctg gagaatgtca ggtgcggcac
     7201 tgaagaacga ttgccttttg tttccatcgg agaacgtcgc cactgtcgcc gattgcagcg
     7261 gcgttgccgc tgtcagagac gtcgtacaac gatcatgttc gcggcgagga cccagctctc
     7321 accgtcgtcg aagctattgc gttctcgcca cggtaaccct cgctgccggt ccacgggcca
     7381 acattcttct gaaaagcgtc ggccttcgct ttggtggccg ctccatctat ttcgctcagt
     7441 tcctgctggg gatttatggc ggctatttcg gcggcgcggt cggcctgatg atgctcgcgg
     7501 tgtggagtct cgcgacttcg gcagacctcc gccagctgac gccgttgcgg accgtaatgg
     7561 tagcggctgc gaacggcgct gcagtcctgt gcttcgccgc tactggcctc gtgagttggg
     7621 ccgagacctc aatcgtgatg atcggcggga tactcggggg ctatgtcggt gcgcggctga
     7681 gccgtcggct gccggcgaat gttctccggg gcgccaccct gtcgatcagc gttgtcacca
     7741 ccgctgtttt cttcgcacgc gcctactgag accggtcaac cacccttggc ccagaccctg
     7801 agggttgacc gcccatggat ctatttatcg ataacgacaa ctggagttcc ccccgtgtct
     7861 gaccgttcac atccctttgt cctggcgccg gacgccgtca aagccctgat ggacctggac
     7921 gcttaggtgg acggcgccgg gcttcccgcg ccccttgttg aactcgtccg aattcgaaca
     7981 tctcaactga acaactgcgc ctactgcgtc gaaacccatg tgaaaatggc cctcgacagc
     8041 cgcgaggatc cgcagcgttt ggaactgcta tccacgtggc gggaaagctc ggtgttcacg
     8101 cacgcgagcg tgctgcactc gcctggagcg aggcggtgac attgatctct gccgggcccg
     8161 tgggcgacga tatctacgca gaagccgtgg gacagttcag cgaggcggag ttggtccgcc
     8221 tcaccgtcgc ggtcgcggca atcaacgcct ggaaccgtct ggcagtcagt ttcaacctca
     8281 gtgatgctcg cagtggggca gcgcgtgttc ggaatgatct cggctccacg atgcacggct
     8341 agcttccgtt ttcgccgttg tcgagcgccc tctcaggggc atccgaactg ttcccagaag
     8401 gaccaacacc atgactatgc aactcatcaa tccacccggg tccgagccgc tttatgaggc
     8461 ataccagttg tcacaggcgg tcaggattgc agatacggtc cacgtgtcgg gtcaggtcgg
     8521 ctacgatgcg ggcggcattc ccgaaggcaa ggaggaccag atccgccttg catttggcca
     8581 cctgaaggcg gttttggcct gcgctggagc gtccatggcc gacatcgtat cgctgacgtc
     8641 ctatcacacc gacccagcgg acttcgaagg cttcaaaagg gtgcaacgcg agttcatccc
     8701 gttgaactac cccgcccaca cagccgtcgg cgtgtccgct ttgctgatgc ctgcgctgaa
     8761 gtgcgaaatc gcagcgattg ccgtcatagg cagcagggcc gtgtcctaag cgcgccatct
     8821 cccggcttgg tcgcccgctg gccatcgttt caactgcaat gacggaaacc ctgatgccga
     8881 agatcgaaat tgcgacagcg ccgaccagat tgggttcagg ctatccgcaa ccttacgacc
     8941 agcagtgcgc cggccgtcgc cgctgggcat tgggtgacgc agccgggttg agccagttcg
     9001 gagtcaacct gttgcggttg cctccccaac agtggtcaag ccagcggcat tggcactccg
     9061 ccgaggacga gtttgtctac gtccttgaag gtgaggtggt tttgtacacc aacgccggag
     9121 cacaggtgat gaa
//
LOCUS       DASVTM010000050         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_218128,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000050 DASVTM010000000
VERSION     DASVTM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218128"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VF633_01310"
     CDS             complement(<1..184)
                     /locus_tag="VF633_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469725.1"
                     /translation="MIKPILALAATGVLLVACGNNNETTGPVEGPAVASSEAPRNPAV
                     DTEDTSAKEALTPGANS"
     gene            complement(358..2004)
                     /locus_tag="VF633_01315"
     CDS             complement(358..2004)
                     /locus_tag="VF633_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8469726.1"
                     /translation="MFLSLSRRQAMSSAVGLGAVACPAFASALTQADASTPGDATALA
                     EAVDVYVKRAMAAFPDQPAISIAVVKDGRTVLARGYGVRKMGEAQTVDEHTLFAIASN
                     TKNVTAAALAMMVDEGRVKWDEPVRTYLPDFRLSDPYISEHITVRDTLSHRAGFGLGA
                     GDLLFWPNSDRTREEVVAQAAFVPIEDGFRANYHYCNLMFVVAGQVLAAASGMRWEEF
                     VQKRIFDRVGMTGTVPMATLADPAKSALPHARVGPPLRTHGPMTLIAESIAEVWNWDS
                     AAAAGGICTNPVDWAKWINVRLAMGKLPDGSRLFSESVAREMWKPNIIISGTEGPTAA
                     MPGRAIASTYAMGYQVQDYRGERYISHGGGSPGGISATVMIPGRNVGFSIFSNAEEGM
                     LLRSLRSGIADLCMGPDRSGADFDWITDSLRLAAEAETRAIAAAAGIAAKQAQGARPS
                     LPLEAYAGTWRDPWYGDITIQSQDGGLWLAFTHTSALKGVLEPYDGETMRTRFGDKRE
                     EDLLITFALVDGQPATATMKALSPDADFSYDYQDLRLTRV"
     gene            complement(2216..2290)
                     /locus_tag="VF633_01320"
     tRNA            complement(2216..2290)
                     /locus_tag="VF633_01320"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2256..2258),aa:Gly,seq:gcc)
     gene            complement(2427..3209)
                     /locus_tag="VF633_01325"
     CDS             complement(2427..3209)
                     /locus_tag="VF633_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8469727.1"
                     /translation="MKLDSSMAAVVTGGASGLGEGTARAIAATGAKVALFDMNEEKGE
                     AIAAEIGGVFCKVDVTSDEEVAAAFAKARAAHGQERLTVNCAGIATGQKTVSRKKDTG
                     EIRAHDMASFERTVRINLFGTFRVLSQSALGMVTLEPMEDGERGLIVNTASVAAQDGQ
                     IGQAAYSASKGGVYAMTLPVARDLAQDGIRVNTILPGIMWTPMMAGMDQKIQDALAAA
                     IPFPSRLGRPADYASLVLELARNVYINGECIRLDGAIRLAPR"
     gene            3264..3671
                     /locus_tag="VF633_01330"
     CDS             3264..3671
                     /locus_tag="VF633_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX8469728.1"
                     /translation="MPTRSQPVTPDDVLDPAKALVPSVMRVNEVRVQRGFWPKIVRTA
                     AHIPFVDQALSVYYAARDPATPTAAKGMMLGALAYFVMPLDAIPDIFAGIGFTDDAAV
                     ITALIATLGANIKRRHRDQAGKTVERLKDRQEG"
     gene            complement(3716..4717)
                     /locus_tag="VF633_01335"
     CDS             complement(3716..4717)
                     /locus_tag="VF633_01335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8469729.1"
                     /translation="MRAVLSKVVGGPESLVVEEVLDPTPGKGQVIIEVKAVGINFPDT
                     LIIEDKYQFKPARPFSPGAEVAGVIEAVGEGVKGLFKGDRVIAVPGWGGLVERLAVPA
                     ASVIKMPDAMSFEEGAALVMTYGTSYYALKDRAQLKAGETLLVLGAAGGVGAAAVELG
                     KAMGARVIAAASTNDKVQFALELGADTGLIYPSGEMDKTAQKELSGELKLASGRDGPD
                     VVYDGVGGGYAEPALRSMDWYGRYLVVGFPAGIPSFPLNLTLLKSVSVIGVFWGAAVA
                     RDPAGHAANIADLLTFYAEGKIKPRIFKTYPLERAHEAIKALGDRAAMGKIVVTVES"
BASE COUNT          870 a         1654 c         1617 g          872 t
ORIGIN      
        1 aggagttggc gcccggcgtg agcgcctcct tcgcggacgt gtcctccgta tcgaccgccg
       61 gattacgcgg cgcttcggac gaggcgacag ccgggccttc aacaggtccg gttgtctcat
      121 tgttgttgcc gcaggcgacc agcaggacgc cggtcgcagc aagggcgagg ataggtttga
      181 tcatggagct ttcccttcca aggttgcaga ctggaaagct ctcaccacgc caggcgttcc
      241 gcacagaacg gcgatctccg gtcaaaatgg agagttccgt ccgatgcgcc tccctgcggg
      301 tccacccctc ccgtgacctt tgagggcccg taccgcccgg actgagccga cgtgcgatca
      361 gacgcgcgtc agtctgagat cctggtagtc gtacgagaaa tcagcgtcgg ggctcagcgc
      421 cttcatcgtc gcggtcgcag gctgaccgtc gacaagggcg aaggtgatca gcaaatcctc
      481 ttcgcgtttg tcgccgaacc gcgtccgcat ggtctcgccg tcatagggct ccagaacgcc
      541 cttcaacgcg gaagtatggg tgaacgccag ccacagacct ccgtcctgcg actggatcgt
      601 aatgtcgccg taccaagggt cgcgccatgt tccggcatat gcctcgagcg gcagggacgg
      661 cctggcaccc tgagcctgtt ttgctgcgat cccggcggcg gcggcgatgg ccctggtctc
      721 ggcttcagca gccagtcgaa gcgagtccgt gatccagtcg aagtccgcgc cggagcggtc
      781 cggacccatg cagaggtcgg cgatcccgct tcgcagcgac cgcagcagca tcccctcctc
      841 ggcattggag aagatcgaga agccgacgtt gcggcccggg atcataaccg tcgccgagat
      901 gccgcccggc gaacctccac cgtggctgat gtagcgttcg ccgcgatagt cctgcacctg
      961 gtatcccata gcgtaggtgg atgcgatggc tcgccctggc atggcggccg tgggtccctc
     1021 ggtgcccgag atgatgatgt tgggcttcca catctctctg gcgacggatt cggagaacag
     1081 ccgcgaaccg tccggcaact tgcccatcgc cagccggacg ttgatccatt tggcccagtc
     1141 gacaggattg gtgcagatgc cacccgcagc cgccgctgaa tcccagttcc agacctcggc
     1201 gatcgactcg gcgatcagcg tcatggggcc gtgggttcgc aacggcggcc cgacccgggc
     1261 atgaggcagc gcagacttcg ccgggtccgc cagcgtcgcc atcggcacag tcccggtcat
     1321 gccgacgcgg tcgaagattc gcttctggac gaactcctcc caacgcatgc cggaagcggc
     1381 agccagcacc tgtcccgcca ccacgaacat cagattgcag tagtgatagt ttgcccggaa
     1441 cccatcctcg atcggcacga aagcggcctg cgcgaccact tcctcgcgcg tgcggtcgct
     1501 gttgggccag aacagcagat ctccggcgcc cagcccgaac cctgcgcggt ggctcagcgt
     1561 atcgcgcacc gtgatatgct cgctgatata cgggtccgac agcctgaaat cgggcagata
     1621 ggtccggacc ggttcgtccc acttcaccct gccttcgtcg accatcatgg cgagggcagc
     1681 ggccgtgaca ttcttggtgt tcgaggcgat ggcgaacagc gtgtgttcgt cgacagtctg
     1741 cgcctcgccc atcttgcgaa cgccatagcc gcgcgcgagt acggtccgtc cgtccttgac
     1801 cactgcgatc gagatcgcgg gctgatccgg aaacgcggcc atcgcccgct tcacatagac
     1861 gtccacagcc tccgccagag cagtcgcatc gcccggcgtt gacgcgtcgg cctgcgtgag
     1921 agccgacgcg aatgcgggac aggcgacggc gcccagaccc accgcagatg acatcgcctg
     1981 acgacgcgaa agactaagaa acatgcgaaa cgctccacca acaagacagg acgctactca
     2041 tcgatcagca ttggcgccag accgggcagc ggcttcacca ctgtcgccgc ggaccacgtt
     2101 cccggtgaaa tctgcatcaa ggacaatgtc acgcttaagc aacatcgcga acaatcctgc
     2161 cggggcccgg aaacagaaaa gcccgatccg tgaggaccgg gccttctggt tcgactggag
     2221 cgggcgaaga gattcgaact ctcgacccca accttggcaa ggttgtgctc taccactgag
     2281 ctacgcccgc actccgtaac ggtccccgaa gggcgatccg tctcgtcgag aggcgggcgt
     2341 atagcggacg cttgcggcat acggcaaggg tctttttctc cgaactccgg attatctcga
     2401 ggccggcccg acagcctcgt cttccgtcaa cgaggcgcga gacggatcgc cccgtccaga
     2461 cggatgcatt ccccgttgat gtagacgttg cgggccagct ccagcaccag cgaggcgtag
     2521 tccgccggac ggccgaggcg gctggggaac gggatggcgg cggcgagggc gtcctgaatc
     2581 ttctgatcca tgccggccat catcggcgtc cacatgatgc cgggcaggat ggtgttcacc
     2641 cggatgccgt cctgggccag atcgcgggcc actggcaggg tcatggcgta gacgccgccc
     2701 ttggatgccg aataggccgc ctggccgatc tggccgtcct gtgcggccac tgaggccgta
     2761 ttgacgatca ggccgcgctc gccgtcctcc ataggctcca gagtgaccat gccgagcgcc
     2821 gactgcgaca ggacccggaa ggtgccgaac aggttgatgc gcacggtgcg ctcgaagctg
     2881 gccatgtcgt gggcgcggat ctcgccagtg tccttcttgc gcgagacggt cttctggccg
     2941 gtggcgatgc cggcgcagtt gacggtcagg cgctcctggc cgtgggcggc gcgggcctta
     3001 gcgaaggcgg cggcgacttc ctcgtccgag gtcacgtcga ccttgcagaa gacgccgccg
     3061 atttcagcgg cgatggcctc gcctttttcc tcgttcatgt cgaacagggc gaccttggcg
     3121 ccggtggccg cgatggcgcg cgccgtgcct tctccgagac cggaagcgcc gccggtgacg
     3181 actgcggcca tcgaactgtc gagtttcatg cgaagagtcc ctatcttgtc tgaaccatgg
     3241 atgttccgag gggtttagcg gcgatgccga cccgatcaca acctgttacg cccgacgatg
     3301 tgctggaccc cgccaaggcg ctggtcccgt ccgtgatgcg ggtcaatgaa gtcagggttc
     3361 agcgtggatt ctggcccaag atcgtgcgca ccgcggcgca catccctttc gtggatcagg
     3421 cgctgagcgt ttattacgcc gcccgagacc ccgcgacgcc gacggcggcc aaggggatga
     3481 tgctgggggc gctggcctat ttcgtcatgc ccctcgacgc gattcccgac atcttcgccg
     3541 ggattggctt caccgacgac gctgcggtga tcaccgccct gatcgcgacg ctgggggcca
     3601 acataaagcg ccgccaccgc gatcaggcag gcaagacggt cgagcggctc aaggatcggc
     3661 aggagggcta gccgcgaaca cgccgactgg aacgtcggcg tgttcgatca ggccgtcacg
     3721 actcgacggt caccacgatc ttgcccatgg cggcgcggtc gccgagagcc ttgatggctt
     3781 cgtgggcccg ttccagcgga taggtcttga agatacgagg cttgatcttg ccctctgcgt
     3841 agaaggtcag caggtcggcg atgttggcgg cgtgaccggc gggatcacga gcgaccgcag
     3901 cgccccagaa gacgccgatg accgacaccg atttgagcag ggtcaggttc agcgggaacg
     3961 acggaatgcc cgccgggaag ccgaccacca gatagcggcc gtaccagtcc atcgagcgca
     4021 gggcgggctc ggcatagccg ccgccgacgc cgtcatagac cacgtcggga ccgtcgcggc
     4081 ccgaggccag tttcagctcg ccggacagtt ctttctgggc cgtcttgtcc atctcgccgg
     4141 acggatagat caggccggtg tcggcgccga gttcgagtgc gaactggacc ttgtcgttgg
     4201 tcgaggcggc ggcgatgacg cgggccccca tggccttgcc cagttcgacg gcggcggcac
     4261 cgacgccccc tgcagcgccg agcaccagca gggtctcgcc ggcctttaac tgggcccgat
     4321 ccttgagcgc gtaataggag gtgccgtagg tcatcacgag agccgcgccc tcttcgaacg
     4381 acatggcgtc gggcatcttg atgacgctgg cggcggggac ggcgaggcgt tcgaccagcc
     4441 cgccccagcc gggaacggcg atgacgcggt cgcccttgaa caggcctttg acgccctcgc
     4501 ccacggcttc aatcacgccg gccacctcgg cgccgggcga gaagggacgc gccggcttga
     4561 actggtattt gtcctcgatg atcagggtgt cggggaagtt gatgccgacc gccttgacct
     4621 cgatgatcac ctgaccctta ccgggggtcg gatcgaggac ctcctcgacg accagagact
     4681 cgggaccacc gacgaccttg gatagaactg cgcgcatgct tgtttcctcg ttcctcggac
     4741 ggcctgccgc tcttgaggcc gttctgtttg gcccaccgcg cttcgcgctg cttcaggccg
     4801 gttgatctgg tttggcgctg acccccttgc cgccggtgaa cggtggcgcg cgcgtctccg
     4861 ggacgctaat cagccgccag caagattgcc agacccgaat gggagccgcc gccaatgagc
     4921 tcctgcagta agcctttccg gagacgttca tgactcgccc agccctgatc ctgcacggcg
     4981 gcgccggcgc ccgacgtgaa cgcgattaca gcc
//
LOCUS       DASVTM010000051         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_855554,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000051 DASVTM010000000
VERSION     DASVTM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855554"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="VF633_01340"
     CDS             complement(<1..1740)
                     /locus_tag="VF633_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX8469730.1"
                     /translation="MRKLLATAAAIAPLLVATGVQAEVVVSTTRTTPIQTSNATGTAA
                     DNIRFASGGGTNLTSGTAVTVDSSNTLTIDGGAAVNIQTAADNSVAVLVNGGTSTTIL
                     INGQVLVSDTLTTYPDTDSDGDVDGPWAVGTNRYGVRLAGAGAVTGNVTIGSSGVVQV
                     EGNNSIGLAVDTNLIGNLNSYGTITAVGDNSVAVRTTAPITGSVTVDGTVTSNGGNSS
                     AAAILGDVSGRLTLQGAMTSSGYRYNQQGTDAFIKTLDPDDLLQTASTVIVGANVGGG
                     VVFDTAPADKDTANTDEDGDGRVDTTEGTASITAYGAAPAVTVGSATRSVTLGVAGTG
                     DNAYGFINRGSIVGNGVYDGVSSNGVLFGVAGGQAVNVAGGILNTGTMAVLSSEADAT
                     TVHLAAGASTPILRNDGFITAASATSLASTVNAIRIDAGASLPSFTNNGSLLATSGGG
                     TANVTALVDLSGTLTNVLNTGSFQASLVANEAGDPLKGSTVAMDLRANTTGVTVTQTG
                     ILSDTTSTLVDTDNDGVDDVDEPSLVGNVLLGSGADTVNLQNGIMLGDIAFGVGADNL
                     NITGGGYVRGALSD"
     gene            1947..2876
                     /locus_tag="VF633_01345"
     CDS             1947..2876
                     /locus_tag="VF633_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8469731.1"
                     /translation="MTTDSTTIAGSGRAQRAPSIEGPANSGNGVTMLLFSLALMIGGP
                     VLAVQMAGEIMAVGAGVLAAVLGLLILCGLYSQQPNEATAILLFGDYRGTDRITGLRW
                     VVPWYSRKKISLRARNITSDKLKVNDKRGNPVEIAANVVWRVSDSAQALFDVDDYQAF
                     VNIQVDTGLRDIASHYAYDHGEQVEGVDQELTLRADAEDVAARLRDELAKRCSVAGVA
                     VDEARLAHLAYAPEIAGAMLRRQQAEAILSARRLIVSGAVGMVETALADLSTRGVVDL
                     DEDRKAAMVSNLLVVLCADREAQPVVNTGTLYG"
     gene            2906..3073
                     /locus_tag="VF633_01350"
     CDS             2906..3073
                     /locus_tag="VF633_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469732.1"
                     /translation="MRASPGVMAAVERLAAADLRSVNAQIEVLLREALARRGVSVTEA
                     RQNDQGPDDTA"
     gene            3186..>3596
                     /locus_tag="VF633_01355"
     CDS             3186..>3596
                     /locus_tag="VF633_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8469733.1"
                     /translation="MSLVDIDVTESDLRLIPGWDEEVAETLRILKASTSDPRPRLVDT
                     TMLYAPRSGGVKRYLESKKSWLAENRPGVAHSLVVPGARHDAGADGIIKLRATKLPFG
                     DGYRWPSSVKRWGAWVASMKPSIIEAGDPYTPGQG"
BASE COUNT          611 a         1118 c         1190 g          677 t
ORIGIN      
        1 gtccgacagg gcgccacgca catagccgcc gccggtgatg ttaagattgt ccgctcccac
       61 gccgaaggcg atgtcgccca gcatgatgcc gttctgaagg ttaaccgtgt cggcgccgga
      121 tcccagcagg acgttcccga ccagcgaagg ctcgtcgacg tcatcgacgc cgtcgttgtc
      181 agtgtcgacg agggtgctgg tggtgtcgct cagaatgccg gtctgggtaa cggttacgcc
      241 cgttgtgttg gcccgcaggt ccatggcgac cgtggaaccc ttcaagggat cgccggcctc
      301 gttggcgacg aggctcgcct ggaaggatcc agtgttcagg acgttggtca gggtcccgct
      361 cagatcgacc agggcagtga cgttcgcggt tccgccgcca gatgtagcca gcagcgatcc
      421 gttgttggtg aaagatggaa gactggcgcc ggcgtcgatc cggatcgcat tgacggtgct
      481 ggccaagctc gtcgcggatg ctgcggtgat gaagccgtcg ttgcgcagga tcggggtgct
      541 tgcgccggcc gccagatgga ctgtagtggc gtcggcttcg ctggagagga ccgccatagt
      601 cccggtgttc aggatgccgc cggcaacatt gacggcctga ccgcccgcga cgccgaacag
      661 gacgccgttg ctgctgacgc catcatagac gccattaccg acaatcgagc cacggttaat
      721 gaagccatag gcgttgtcgc cggtcccagc aacgccaagg gtcacggatc gagttgccga
      781 acccacggtc accgctggcg cagcaccata ggcggtgatg ctggcagtgc cttcggtcgt
      841 atcgacccgg ccgtcgccat cctcgtcggt gttggccgta tccttgtcgg caggggcggt
      901 gtcgaatacg acgccaccgc cgacattggc gccgacgatg acggtcgagg ccgtctgtag
      961 caggtcgtcg gggtccagcg tcttgatgaa ggcgtccgtg ccttgctggt tgtagcgata
     1021 gccgctcgac gtcatcgcgc cttgcagcgt cagccgaccg gaaacgtcac cgaggatcgc
     1081 tgccgcagaa gaattgccgc cgttggaagt gaccgtgccg tcgaccgtga cactgccggt
     1141 gatgggggcg gtcgtgcgga cggcgacgga gttgtcgccg acggcggtta tcgtgccgta
     1201 gctgttcaga ttgccgatca ggttcgtgtc tacggccagg ccgatcgagt tgttgccttc
     1261 gacctgcact acgccggacg agccgatcgt tacattaccc gtcacggccc ccgcgccggc
     1321 cagccgcacg ccatatcggt tggtgccgac agcccatggg ccgtcgacat cgccgtcgct
     1381 gtcggtgtcg ggataggtcg tcagagtgtc ggagaccagt acctgaccgt tgatcaggat
     1441 ggtggtgctc gtcccgccgt ttaccagcac ggcaaccgaa ttatcggccg cagtctggat
     1501 gttgacggcg gcgccgccat cgatcgtcag ggtgttgctg gaatcaacgg tcacggccgt
     1561 accggacgtc agattcgttc cgcctccgct ggcgaagcgg atgttgtccg ccgccgtgcc
     1621 ggtggcgttc gaagtctgga tgggagtcgt gcgggtggtc gagacgacga cctccgcctg
     1681 aacgccggtg gcgaccagca gcggagcaat cgccgcagcg gtcgcgagta gtttacgcat
     1741 tcggtagaaa cccctgtgaa cacacacgcg cacggtcatc ggggacccct tatcagggcc
     1801 cgcgcgacca cgcctcaagt ttcgcgaatt tgaggcgatt gcaaggccgg agcgccgtgt
     1861 ttcaaagggc gttggccaga tgcagcttgt cggatacgca atcaatctga tatcacttag
     1921 atagtgaata gcggagggct gagaccatga cgaccgattc aaccacaatc gccggcagtg
     1981 gccgggcaca gcgggcgccc tcgatcgagg gaccggctaa ctctggcaat ggcgttacga
     2041 tgttgctgtt cagcttggcc cttatgatcg gcgggccggt gcttgctgtc cagatggcgg
     2101 gagagatcat ggccgtcggc gccggggtcc tcgccgccgt cttggggctt ttgatccttt
     2161 gcgggttgta ttcccagcaa ccgaatgagg cgaccgccat cctgctgttc ggtgactacc
     2221 gcggcaccga tcgcatcacc ggtcttcgct gggtcgtgcc atggtacagc cgcaagaaaa
     2281 tctcgctgcg cgccagaaac atcacgagcg acaagctcaa ggtgaacgac aaacgcggca
     2341 acccggtcga gatcgcggcc aatgtcgtct ggcgcgtgtc cgattccgcc caggccctgt
     2401 tcgacgttga tgactatcag gcgttcgtga atatccaggt cgacactggc ctgcgcgaca
     2461 tcgcttccca ctatgcctac gaccacggcg agcaggtcga aggtgttgat caggagctga
     2521 ccctgcgcgc cgatgccgag gacgttgcgg cccgcttgcg tgacgaactg gcgaaacgct
     2581 gcagcgtcgc gggtgtcgcc gtcgatgaag cccgtctggc ccaccttgcc tatgccccgg
     2641 aaatcgccgg ggcgatgttg cgccgtcagc aggctgaggc aatcctgtcg gctcgtcgcc
     2701 tgatcgtctc gggcgctgtt ggcatggtcg agacggcgct cgccgatctc agcactcgtg
     2761 gcgtcgtcga tctcgacgag gaccgcaagg ccgccatggt ctccaacctg ctggtcgtcc
     2821 tgtgcgccga ccgcgaagct cagcccgtgg tcaacactgg cacgctctac ggctgatggc
     2881 ggcaaagccc cgcaaaccat tcttgatgcg cgcctctccg ggcgtgatgg cggccgtcga
     2941 acgtctggcg gccgccgatt tgcgctcggt caacgcccag atcgaggtgc ttctgcgcga
     3001 ggctttggcg cggcggggcg tctctgtaac cgaggcacgg cagaacgatc agggacctga
     3061 cgataccgct tgagcttggc gttgcgcggg tttaggggcg cttgatggtc tcgcgtaaaa
     3121 cagctgcccg catccaaact cgccagtcgg gcgttgcccg gtccagcaga gcggagaagt
     3181 cccccatgag cctggtcgat atcgacgtca ccgaatccga cctgcgcctg attccaggtt
     3241 gggacgagga agtcgccgag acgctcagga tcctgaaggc ctcgacctcc gatccgcgtc
     3301 cccggctggt cgacaccacc atgctgtatg cgccgcgttc cggcggggtg aagcgctacc
     3361 tcgagtccaa gaagagctgg ctggccgaga atcgccccgg cgtcgcccat tcgcttgtcg
     3421 tgccgggcgc ccgtcacgat gcgggcgcgg atggaatcat caaacttcga gcaaccaagc
     3481 tgccgttcgg cgacggctac cgctggccgt cctcggtgaa gcgctggggg gcctgggtcg
     3541 cctccatgaa gccgtcgatc atcgaggccg gcgatcccta tacccccggt cagggc
//
LOCUS       DASVTM010000052         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_377525,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000052 DASVTM010000000
VERSION     DASVTM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377525"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VF633_01360"
     CDS             complement(<1..959)
                     /locus_tag="VF633_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8469734.1"
                     /translation="MLQITDLTFNAWGRKFLVDASVSLPPGSKVGLVGRNGIGKTTLF
                     KLILGELHAGGDEISLPKTARIGSVDQEHPATPVSVIDTILEADVERHTLLGRLETAE
                     PEEMGDIWSRLIEIDADAAPARASEILVGLGFDQENQQRPMSEFSGGWRMRVALAAAL
                     FAEPDMLLLDEPTNYLDLEGALWLEARLKKYPHTALIISHDREMLNEVCTHILHLANH
                     TLTIYTGNYDAFEKARAEKSRLQMSAKAKQDAERAHLQSFVDRFKAKASKAAQAQSRM
                     KRLEKMQPVATTIEERVAPFTLPSPPRPLAPPLIRLERANVGY"
     gene            1099..1518
                     /gene="ndk"
                     /locus_tag="VF633_01365"
     CDS             1099..1518
                     /gene="ndk"
                     /locus_tag="VF633_01365"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX8469735.1"
                     /translation="MTERTFSIIKPDATRRNLTGAVNAVIEGAGLRIVAQRRVKLSTD
                     QAKKFYEVHAERPFYGELVEQMTAEPVVVQVLEAEGAIAKYREVMGATNPKDAAEGTI
                     RKQFALSIGENSVHGSDSAENAAIEIAQFFTDDQIVG"
     gene            1640..1846
                     /locus_tag="VF633_01370"
     CDS             1640..1846
                     /locus_tag="VF633_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX8469736.1"
                     /translation="MKTITKLAPLVGVVGLLAACGTTMEQRAATGAIGGAVAGQVIGG
                     NTKSTVIGGVAGAAVGAATTPRRR"
     gene            complement(1920..>2838)
                     /locus_tag="VF633_01375"
     CDS             complement(1920..>2838)
                     /locus_tag="VF633_01375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX8469737.1"
                     /translation="SSKVAMEGLVKNLVAAMTERLKRLEWMSPETKEQALYKIAHFGV
                     KVGYPDEWRSYDGLTISPTDLYGNTERAGAFEWAFQTSKLGRPVNPKEWGMTPQTINA
                     YYNPPRNEIVFPAAILQAPFFDPNADMAVNYGAIGAVIGHEITHGFDDQGRKVDGNGV
                     LRDWWTAEDATKFEARAKVYGAEYDATFPLPGMHVNGAATMGENIADLGGLLMALDAY
                     HLSLNGQPAPVINGLSGDQRLFLGWAQVWRAKQRDAALQQQLATDPHSPASVRASVPV
                     RNVQAWYDAFGVKPGDEQYLAPADRAVIW"
BASE COUNT          500 a          877 c          944 g          517 t
ORIGIN      
        1 tcgtagccga cgttggcgcg ctccagccgg atcagcggcg gcgccagagg gcgaggcggc
       61 gagggcagag tgaacggagc gacacgctcc tcgatggtgg tggcgaccgg ctgcatcttc
      121 tccagccgct tcatgcgcga ctgggcctgc gcggccttgg acgccttggc cttgaagcga
      181 tcgacgaagg actgaaggtg ggcgcgctcg gcgtcctgct tggccttggc cgacatctgc
      241 aggcgcgact tctcggcccg agccttttcg aaggcgtcgt agttgccggt gtagatcgtc
      301 agcgtgtggt tcgcgaggtg caggatgtgg gtgcagacct cgttcagcat ctcgcggtcg
      361 tgggagatga tcagggcagt gtgcggatat ttcttcagcc gggcctcgag ccacagggcg
      421 ccttcgaggt cgaggtagtt ggtcggttcg tcgagcagca gcatgtccgg ttcggcgaac
      481 aaggcggcgg caagggcgac gcgcattcgc cagccgcccg aaaactcgga catcggtcgt
      541 tgctggtttt cctgatcgaa gcctaggccg acgaggattt cagaggcgcg ggcgggggcg
      601 gcgtcggcgt cgatctcgat caggcgcgac cagatatcgc ccatctcctc gggctcggcc
      661 gtttcgagac ggcccagcag ggtgtgacgc tcgacatcgg cctcaaggat ggtgtcgatg
      721 acgctgacag gcgtggccgg gtgttcctga tcgaccgagc cgatgcgggc cgtctttggt
      781 aggctgattt cgtcgccgcc ggcgtgaagc tcgcccagaa tcagcttgaa cagcgtcgtc
      841 ttgccaatgc cgttgcgacc gaccagaccg accttggagc ccggcggcag gctgacggag
      901 gcgtcgacga ggaatttgcg gccccaggcg ttgaacgtca ggtctgtaat ctggagcatg
      961 acgtggaaac ccggggtttg tccctcaatc cgcgagccat cgggcgcgct tcagggacac
     1021 aaaagagaga tagttggaaa ctagggaggg cgcgtctata agcccccacc tccacaatcc
     1081 caaacaagaa gtctcaccat gaccgaacgc accttctcga tcatcaagcc cgacgcgacc
     1141 cgccgcaacc tgacgggcgc cgtcaacgct gtgatcgaag gcgccggcct gcgcatcgtg
     1201 gcccaacgcc gcgtcaagct gtcgaccgac caagccaaga aattctacga agtccacgcc
     1261 gagcgcccgt tctatggcga gctggtcgag cagatgaccg ctgagcccgt cgtcgtccag
     1321 gttctggaag ccgaaggcgc catcgccaaa taccgtgaag tcatgggcgc aacgaacccg
     1381 aaggacgctg ccgaaggcac gatccgcaag cagttcgccc tgtccatcgg tgaaaactcg
     1441 gtccacggtt cggactccgc cgagaacgcc gcgattgaaa tcgcccagtt cttcacggac
     1501 gaccaaatcg tcggctaacg ccgtctgtgc tttttaaagc gcggtagacg acatagcggc
     1561 ccgtcccggg gaacctggga cgggccgttt cgtttcagtt gccaagcttc actcccccag
     1621 aagttttgga gaactcgaaa tgaagacgat caccaaactc gctcccttgg tcggcgttgt
     1681 gggcctgctg gccgcttgcg gcactacgat ggaacaacgc gctgcaaccg gcgccatcgg
     1741 cggcgccgtc gccggtcagg tcatcggcgg caacaccaag tcgaccgtca tcggtggtgt
     1801 cgccggtgcg gccgtcggcg ctgcgactac cccgcgtcgt cgctaagcct tacagctaca
     1861 catgaaagcg gccccgggag cgatcccggg gccgttgtca tgtccaggat ctggtgaagc
     1921 taccagatca ccgcccggtc ggcgggggcg aggtactgtt cgtcgccggg cttgaccccg
     1981 aaggcgtcgt accaggcctg gacgttgcgc accggcaccg aggcgcgaac cgaggccggc
     2041 gagtggggat cggtcgccag ttgctgctgc agggcggcgt cgcgctgctt ggcacgccag
     2101 acctgagccc agccgaggaa caggcgctgg tcgccgctca ggccattgat gaccggagca
     2161 ggctggccgt tcagcgacag gtgataggcg tcgagcgcca tcagcagacc gcccagatca
     2221 gcgatgttct cacccatggt cgctgcgccg ttcacatgca tgccgggcag ggggaaggtg
     2281 gcgtcgtatt cggcgccgta gaccttggcc cgggcctcga acttggtcgc gtcctcggcc
     2341 gtccaccagt cgcgcagcac gccgttaccg tcgaccttgc ggccctgatc gtcgaagccg
     2401 tgggtgatct catggccgat gaccgcgccg atagcgccgt agttgacggc catgtcggcg
     2461 ttcggatcga agaacggcgc ctgcaggatg gctgcaggga atacgatctc gttgcgaggc
     2521 gggttgtagt aggcgttgat ggtctgaggc gtcatgcccc attccttggg gttcaccggc
     2581 ctgccgagct tggacgtctg gaaggcccat tcgaaggcac cggcgcgttc ggtgttgccg
     2641 tacagatcag tcgggctgat cgtcaggccg tcatagctgc gccattcgtc gggataaccg
     2701 accttcacgc cgaagtgggc gatcttgtag agcgcctgtt ccttggtctc ggggctcatc
     2761 cattcgaggc gcttgaggcg ttcggtcatc gccgccacaa ggttcttgac cagaccttcc
     2821 atcgccacct tggacgat
//
LOCUS       DASVTM010000053         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_910224,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000053 DASVTM010000000
VERSION     DASVTM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910224"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VF633_01380"
     CDS             complement(<1..677)
                     /locus_tag="VF633_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469738.1"
                     /translation="MINRSRLAALLTGTALMFAAGAVQAQQPEPLPAEVLVDAPDAAK
                     AAFLKDHVVVSGPVSGGLTFAFPPAFYDNKLFLLGESHGVAAPQVLDLELLTHLNARI
                     GLIDYVAEVDPVQGLRLNQYLDSGDEAVLDRVFDYWTRTGAQWGNTAFEDKIRGIRAL
                     NLTLPENRRVHLLGIDAVQDWPLTTAWLAEQGLTVDPAALSAATTNAARATVVLAALP
                     TTSTSPVL"
     gene            complement(761..1036)
                     /locus_tag="VF633_01385"
     CDS             complement(761..1036)
                     /locus_tag="VF633_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEX8469739.1"
                     /translation="MDLFLVLLGLTEVGVFCLFWFDKAQAVDHGQRVRESTLLQAAVV
                     GGLGAWFAQHLLRHKTRKEPFRTRLGVIIILHLALSAGAVWLVLQYG"
     gene            complement(1087..>4047)
                     /gene="carB"
                     /locus_tag="VF633_01390"
     CDS             complement(1087..>4047)
                     /gene="carB"
                     /locus_tag="VF633_01390"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX8469740.1"
                     /translation="VEMIGARAEVIEKAEDRQKFRDAMDKLGLESPRSRVAHTLEEAQ
                     DALAAVGLPAIIRPSFTLAGTGGGIAYNVEEFKEIVLRGLDLSPTTEVLIEESVLGWK
                     EYEMEVVRDKADNCIIICSIENIDPMGVHTGDSITVAPALTLTDKEYQRMRTGSINVL
                     REIGVETGGSNVQWAINPADGRMLVIEMNPRVSRSSALASKATGFPIAKVAARLAVGY
                     TLDELTNDITMVTPASFEPSIDYVVVKIPRFAFEKYPGAEPHLTTAMKSVGEVMAIGR
                     TFQESMQKALRGLETGLSGFDEIEIDGTIDAEDDASARAAVIRALGMPTPDRIRVIAQ
                     AFRHGLTVEEVNAACSYEPWFLRQIADIVREEGHVRVKGLPVDAHDFRKLKAKGFSDA
                     RLGQLTGQTEVAVRKARRDLSVRPVFKRIDTCAAEFASSTAYMYSTYETGALGQVPEC
                     ESEPTENRKAIILGGGPNRIGQGIEFDYCCCHAAFAFADIGVESIMVNCNPETVSTDY
                     DTSDRLYFEPLTEEDVLELIDVERSKGELIGVVVQFGGQTPLKLAHALQADGVPILGT
                     TVDSIDLAEDRERFQVMLNDIGLRQPPNGLARSAEEAAQKAEEVGYPVVLRPSYVLGG
                     RGMMIVHDREQLDRYVGEAMRVSGNDPVLIDHYLNRATEVDVDALCDDETVFVAGVLE
                     HIEEAGVHSGDSACSMPPFSLSPAIIAELKRQTTAMARALKVRGLMNVQFAIEEPHSA
                     EPRIFVLEVNPRASRTVPFVAKTIGQPVAAIAAKVMAGVPLKSFGLADKPYDHIAVKE
                     AVFPFARFAGVDIILGPEMRSTGEVMGLDWKRDGEADMAPAFARAFAKSQIGGGTTLP
                     TTGCAFVSVKDDDKPFIKEAVAALIAEGFKIIATGGTATYLAEQGLAVTPVKKVLEGR
                     PNIVDAMKNGDVQLVFNTTDGKQALQDSFSLRRTALMMKIPYYTTAAGALAASQAIGA
                     IKAGELDVKAIQAY"
BASE COUNT          670 a         1262 c         1411 g          704 t
ORIGIN      
        1 agcaccggcg aggtcgaggt ggtgggcagg gccgccagca ccacggtcgc gcgggctgcg
       61 ttggtcgtcg cggcgctcag ggcggcgggg tcgaccgtga ggccttgttc cgcgagccac
      121 gccgtcgtca gcggccagtc ctgaaccgcg tcgatgccga gaaggtggac gcgacggttc
      181 tctggcagcg tcaggttcag cgcccggatg ccccggatct tgtcttcgaa cgccgtattg
      241 ccccactggg cgccggtccg ggtccagtag tcgaacaccc ggtccagcac ggcctcgtcg
      301 ccgctgtcga gatactggtt caggcgtagg ccctgaaccg gatcgacctc ggccacatag
      361 tcgatcaggc cgatacgggc gttgaggtgg gtcagcagtt cgaggtccag cacctgaggc
      421 gcagcgacgc cgtggctttc gcccagcagg aacagcttgt tgtcgtagaa ggctggcggg
      481 aaggcgaagg tcaggccgcc gctcacgggg ccggagacca ccacgtggtc cttgagaaag
      541 gcggccttgg cagcgtccgg cgcgtcgacc agaacctctg ctggcagagg ttctggttgc
      601 tgggcctgca ccgcgcccgc cgcaaacatc aacgccgtac cggtgagcag agccgccagc
      661 ctggaccgat taatcatctc atttccccaa aacctctgcg tgcatccagc gcaagagaag
      721 ggcggatcgc aagtgaaatc gtagcaatga ctgcaagcga ctagccgtat tgcaaaacga
      781 gccagacggc gccggcgctc agcgcgaggt gcaggatgat gatgacgccg aggcgcgtgc
      841 ggaacggctc cttgcgcgtc ttgtgccgca gcaggtgctg ggcgaaccac gcgccgagac
      901 ccccgacgac ggccgcctgc agcagagtcg actcgcgaac gcgttggccg tgatcgaccg
      961 cttgcgcctt gtcgaaccag aacaggcaga acacgcccac ctcggtcagt ccaagcagga
     1021 cgagaaacaa atccatcagc gacaaattca ttgctcgtca tgctcgaatg agaggggctc
     1081 ggttcgtcag taagcctgga tcgctttcac atccagttcg ccagctttga tcgccccaat
     1141 ggcctgcgag gccgcgagtg ccccggcggc cgtcgtgtag taggggattt tcatcatcag
     1201 ggcggtgcgg cgcaggctga agctgtcctg cagggcctgc ttgccgtcag tggtgttgaa
     1261 gacgagctgg acgtcgccgt tcttcatcgc gtcgacgatg ttcgggcggc cttccagcac
     1321 cttcttgacc ggggtgacgg ccaggccttg ctcggccaga taggtggcgg tgccgccggt
     1381 ggcgatgatc ttgaagccct cggcgatcag ggcggcgacg gcctccttga tgaagggctt
     1441 gtcgtcgtcc ttgacgctga cgaaggcgca gccggtcgtc ggcagggtgg tgccgccgcc
     1501 gatctgggac ttggcgaagg cgcgggcgaa ggcgggcgcc atgtcggcct cgccgtcacg
     1561 cttccagtcg aggcccatga cctcgccggt cgagcgcatt tccgggccga ggatgatgtc
     1621 gacgccggcg aagcgggcga acgggaagac cgcttccttg accgcgatat ggtcgtaggg
     1681 cttgtcggcc agaccgaagg atttcagcgg caccccggcc atgaccttgg cggcgatggc
     1741 ggcgacgggc tggccgatgg tcttggccac gaacggcacg gtgcgcgaag cgcgcgggtt
     1801 cacttccagc acgaagatgc gcggctcggc cgagtggggt tcctcgatgg cgaactgcac
     1861 gttcatcagg ccgcgaacct tgagcgcccg ggccatggcc gtggtctggc gcttcagctc
     1921 ggcgatgatg gcgggcgaga gcgagaacgg cggcatcgaa caggcgctgt cgcccgagtg
     1981 gacgccggct tcctcgatgt gttcgagcac gccggcgacg aagaccgtct cgtcgtcgca
     2041 cagggcgtcg acgtcgacct cggtggcgcg gttcagatag tggtcgatca ggaccggatc
     2101 gttgccggac acgcgcatgg cttcaccgac atagcggtcc agttgctcgc gatcgtggac
     2161 gatcatcatg ccgcgtccgc ccagcacata ggaggggcgc aggacgacgg ggtagccgac
     2221 ctcttcggcc ttttgcgcgg cttcttcggc ggagcgggcc agaccgttcg ggggctggcg
     2281 caggccgata tcgttcagca tgacctggaa ccgctcacga tcttcggcca gatcgatgga
     2341 gtcgaccgta gtgccgagga tcggcacgcc gtccgcctgc agcgcatgcg ccagcttcag
     2401 cggcgtctga ccgccgaact gtacgaccac gccgatcagc tcgcccttcg agcgctcgac
     2461 gtcgatcagc tccagcacgt cttcttcagt cagcggctcg aaatacaggc ggtcggaggt
     2521 gtcgtagtcg gtcgagacgg tctcggggtt gcagttgacc atgatcgact cgacgccgat
     2581 gtcggcgaag gcgaacgccg cgtggcagca gcaatagtcg aactcgatgc cctgaccgat
     2641 ccggttggga ccgccgccga gaatgatggc cttgcggttt tcggtcggct cggactcgca
     2701 ctcggggacc tgtcccagcg cgccggtctc ataggtggaa tacatatagg cggtcgacga
     2761 ggcgaactcg gcggcgcagg tgtcgatgcg cttgaaaacc ggacggacgg acaggtcgcg
     2821 gcgggccttg cgcacggcga cctcggtctg gccggtcagt tggccgaggc gggcgtcgga
     2881 gaagcccttg gccttcagct tgcggaagtc gtgggcgtcc acgggcaggc ccttgacccg
     2941 gacgtggcct tcttcgcgga cgatgtcggc gatctggcgc aggaaccacg gctcatacga
     3001 acaggcggcg ttgacctcct cgaccgtcag gccgtggcgg aaggcctgag cgatgacgcg
     3061 gatgcggtcg ggagtcggca tgcccagagc gcggatgacg gcggcgcggg ccgaggcgtc
     3121 gtcttcagca tcgatcgtgc cgtcgatctc gatctcgtcg aagcctgaca ggccggtttc
     3181 gagaccgcgc agggccttct gcatcgattc ctggaaggtc cggccgatgg ccatgacctc
     3241 gccgaccgac ttcatggcgg tggtcaggtg aggctcggcg ccggggtatt tctcgaaggc
     3301 gaagcgggga atcttgacga cgacatagtc gatcgacggc tcgaacgagg ccggcgtcac
     3361 catggtgatg tcgttggtca gttcatcgag cgtgtaaccg acagccagac gggcggcgac
     3421 cttggcgatc gggaagccgg tggccttgga cgccagagcc gaagaccgcg acacgcgcgg
     3481 gttcatctcg atcaccagca tgcggccgtc ggcggggttg atcgcccact ggacgttcga
     3541 tccgccggtc tcgacgccga tctcgcgcag gacgttgatc gagccggtcc gcatccgctg
     3601 atattcttta tccgtcagcg tcagggcggg ggccacggtg atggagtcgc cggtgtggac
     3661 gcccatcggg tcgatgttct cgatcgagca gatgatgatg cagttgtccg ccttgtcgcg
     3721 gacgacctcc atctcgtact ccttccagcc gagcacgctt tcctcgatca gcacctcggt
     3781 ggtcggcgac aggtcgaggc cgcgcaggac gatctccttg aactcctcga cgttgtaggc
     3841 gatgccgccg ccggtcccgg cgagggtgaa ggacggccgg atgatcgccg gcaggcccac
     3901 cgcggccagc gcgtcctgcg cctcctcaag ggtgtgagcg acgcgcgagc gcgggctctc
     3961 gagcccgagc ttgtccatcg cgtcgcggaa tttctgccgg tcctccgcct tttcgatgac
     4021 ctcggcgcgg gcgccgatca tctcgac
//
LOCUS       DASVTM010000054         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_643667,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000054 DASVTM010000000
VERSION     DASVTM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643667"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1303
                     /locus_tag="VF633_01395"
     CDS             128..1303
                     /locus_tag="VF633_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8469741.1"
                     /translation="MIKRHFFLIAAGVMLALMIVAALLHVAFAEDEGAAKGGPGAGGG
                     RAGGRGQQVSEATVGARSFSDTIRVLGVARGQKSVNITSNATELITAVLFRDGQRVTA
                     GTPLVRLQAREEDADIIQARAQVTQAERNYERYRGLAERGIAPRVTAEDAQTTLETAR
                     ASLAASQARLGDRVIRAPFSGVLGLTTVTSGTLINPGAVITTLDDTDTIRVDFPVPER
                     YLGVLRVGLPLTATADAFGGQAFQGRIALIDTRINEQTRAVTARAEFPNPGGRIRPGM
                     LMRVAIEQGQRQSTAVPEAAIQYEGQGAFVYRITAGQKGSTAQRVEVETGAVEGGFVE
                     IVSGLAVGDKVVGGGLNRIQPNAPVTVAGQGRGQGGRGPAGGKAPAGAPAAAQGAAR"
     gene            1303..4530
                     /locus_tag="VF633_01400"
     CDS             1303..4530
                     /locus_tag="VF633_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8469742.1"
                     /translation="MLSDVAVRRPVFAAVAAIVLCVIGAAAFFFLPVRELPDVDPPVV
                     SISTSYAGASAEVIENRITEPVEQQIAGIQGVDRITSSSRDGRSNVTIEFSLDRNIDD
                     AANDVRDRVARVVGRLPEQADPPEVSKADSDSSPIMILFLRSSSMNRLELTDYADRYL
                     LDRLATVPGVAQAQIFGEQRYAMRIWLNAEAMAARGITVTQVENALNAQNVELPAGSL
                     ESTDKDYTVRVARTYARAEDFAQLPIGTAGSAPAVATANNTTQPSTSGSVGAIQSANR
                     YVTRLGDIARVEEAPAEARRLFRGNGLDQIGLGVTRQAQSNDLAISEGIRSALVDMQS
                     SLPPGTELVVGSDNSVFTSHAISEVWITIGISLALVALVNFIFLGSLRAALIPSVVAP
                     ICLLATFIVLAPLGFSLNLLTLLALVLAIGLVVDDAIVVTENIQRRLDLGEPPLVAAE
                     RGARQVFFAVVATTVVLLAVFAPLLFLPGYVGRLFVELAAAIAAAVAFSAFLALTLSP
                     MLASKILKPAAGSGWVARRVDKAMDTLKNSYGASLDMLVGSRVAVIGVAAMILVVAGG
                     AVGMFFILPSELVPQEDRGRVQVRVQGPEGAGFDYTKKIMLGLEPILAQYKADGEVES
                     YLISAPGFGGGSYNSGNATLILKDWSERKRAADVIAQEINGKLRGQTGAQVNASIPGA
                     FQRGGGGGGGNSNSVEMIVTGSEYEDIYRWLQPVLAASLENPGFSRPRLNYEPNSPRL
                     LVDVDPEKSAALGVSSQEIGRTLETMFGSRRATTYIRGGQEYDVILQTERANRAGVAD
                     LEALYVSTGSGTLVPLASVVDTKTSGDTPDRRRLDRQRSISLQADLNPGETIADAVAF
                     LNAEVAKQPQGVVAIQWGGAARDQQEAGGAVGAAFGLALLLVFLVLAAQFESWITPAV
                     IMLTVPLAAAGGLFGLLMAGSSLNIYSQIGLIILIGVAAKNGILIVEFANQLRDQGRS
                     IREAIIESSTLRLRPIIMTSIATAFGALPLMLWHGAGAGSRQTIGVVIFTGAMFSTLL
                     TLFVVPVIYGALARFTTSPEWTARKIEQWEAQEATANDPVPHAAE"
     gene            complement(4878..>5269)
                     /locus_tag="VF633_01405"
     CDS             complement(4878..>5269)
                     /locus_tag="VF633_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX8469743.1"
                     /translation="VPVPPPLSDVERATMLAALPLPYNQADLENGARIFARCRACHSV
                     TPNGPNMTGPNLYGVIGRVAGTHPGFNYSKPLKAAGFTWDDDRIDHWLTNPRTFLPGN
                     RMSFPGLPNAEDRRDVIAFLSVEGAKR"
BASE COUNT          845 a         1702 c         1802 g          920 t
ORIGIN      
        1 tgcgcaccga cggaaaccgg aacggcgtgc gtttgacaga cattagttgc ctcggcggca
       61 ggaccggcta tgccctgccg gctgcttgca tccgatctca ctgcctgccg cccggagact
      121 atcgcgcgtg atcaaacgac atttcttcct gatcgccgcc ggcgtgatgc tcgccctgat
      181 gatcgtggcg gcgctgctac atgtcgcctt cgcggaggac gagggcgccg ccaagggcgg
      241 cccaggagcc ggtggtggtc gggccggtgg tcgcggacag caggtgtccg aggcgaccgt
      301 gggcgcgcgg tcgttcagcg acacgatccg ggtgcttggc gtggcccggg gtcagaagtc
      361 ggtgaatatc acctcgaatg cgaccgagct gatcacggcg gtgcttttcc gcgatggaca
      421 gcgggtcacg gcgggcacac cgctggttcg acttcaggcg cgcgaggaag acgccgacat
      481 cattcaggcg cgggcgcagg tgacccaggc cgaacgcaat tacgaacgtt atcgtgggct
      541 ggcggaacgc ggcatcgcgc cgcgcgtgac cgccgaggat gcccagacca ctctggagac
      601 ggccagagcc tctctcgccg cctctcaggc gcgtctgggg gaccgcgtga ttcgggcgcc
      661 gttctcaggc gtgctcggcc tcacgacggt tacatcaggc acgctgatca atccgggcgc
      721 agtcatcacg accctcgatg acaccgacac gatccgcgtc gatttccctg tgccggagcg
      781 gtatctgggc gtgctgcgcg tcggcctgcc gctgacggcg accgctgacg cgtttggcgg
      841 acaagcgttc cagggtcgca tcgccctgat cgacacccgc atcaacgaac agacccgcgc
      901 cgtcaccgcc cgggccgagt ttcccaaccc cggcggccgt atccgacccg ggatgctgat
      961 gcgtgtcgcc atcgaacagg gccagcgcca gagcaccgcg gtgcctgagg ccgccatcca
     1021 gtatgagggc cagggagctt tcgtttatcg catcaccgcg ggtcaaaaag gatcgactgc
     1081 ccagcgggtc gaggtcgaga ccggcgccgt cgaaggcggc ttcgtcgaga tcgtgtccgg
     1141 cctcgcggtc ggcgacaagg tcgtgggcgg cggtctgaac cgaattcagc ctaacgcccc
     1201 cgtcaccgtg gccggtcaag gtcgtggtca gggcggccgg ggtccggcgg gtggcaaggc
     1261 tcccgcaggc gctcctgctg cagcacaagg cgccgcacga tgatgctttc cgacgtcgcg
     1321 gtccggcgcc ccgtcttcgc ggcggtcgcg gccatcgtat tgtgcgtcat cggcgcggcg
     1381 gccttcttct tcctgccggt gcgcgaactg cccgacgtgg atccgcccgt cgtctcgatc
     1441 agcaccagct acgcaggggc ctcggctgag gtcatcgaga accggatcac cgaaccggtc
     1501 gagcaacaga tcgcaggcat tcagggcgtc gaccggatca cgtcgtccag ccgtgacggc
     1561 cgctccaacg tgacaatcga gttctcgctc gatcgcaaca tcgacgacgc tgcaaacgat
     1621 gtgcgcgacc gcgtcgcccg ggtggtggga cggttgcctg aacaggccga tccgcccgag
     1681 gtctccaagg ccgacagcga ctcatcgccc atcatgatcc tgttcctgcg gtcttcgtcg
     1741 atgaaccggc tggaactgac cgactacgcc gaccgctatc tactggaccg tctggcgacg
     1801 gtgcctggcg tggctcaggc ccagatcttc ggtgagcagc gctacgccat gcggatatgg
     1861 ctgaacgccg aggctatggc tgcgcgcggc atcaccgtaa cccaggtcga gaacgccctg
     1921 aacgcccaga acgtcgagct tccggccggg tcgctggagt cgacggacaa ggattacacc
     1981 gttcgcgtgg cccgcaccta tgcccgcgcc gaggacttcg cccaactgcc gatcggcacg
     2041 gcgggctcgg ccccggccgt tgcgaccgcc aacaacacca cccagccgtc gacgtcgggc
     2101 tcggtcggcg cgatccagag cgccaatcgc tatgtcacgc gcttgggcga tatcgcccgg
     2161 gtcgaggaag cgccggcaga ggcgcggcgt ctgttccggg gcaatgggct ggaccagatt
     2221 ggtctgggcg ttacgcgtca ggcccagtcc aacgacctgg ctatctccga aggcatccgc
     2281 agtgcgctgg tggacatgca atcgagcctg ccgccgggga cggaactggt cgtcggttca
     2341 gacaactcgg tcttcacgtc tcatgcgatc tctgaggtct ggatcaccat cggcatctcg
     2401 ctggcgctgg tggcgctggt caacttcatc ttcctcggca gcctgcgggc ggcactgatc
     2461 ccgtcggtcg tggcgccgat ctgtctgctg gcgaccttca tcgtgctggc cccgctgggc
     2521 ttctcgctga acctgctgac cctgctggcg ctggtgctgg ccatcggtct tgtggtcgat
     2581 gacgccatcg ttgtgaccga gaacatccag cgacgtctgg acctcggcga gccgccgctg
     2641 gtggccgccg aacgtggtgc gcgtcaggtg ttcttcgccg tcgtggcgac gaccgtggtg
     2701 ctgctggcgg ttttcgcgcc gctgctgttc ctgccgggat acgtggggcg tctgttcgtc
     2761 gaactggcgg cggcgatcgc tgcggccgtc gccttctcgg ccttcctcgc gttgacgctg
     2821 tcgccgatgc tggcgtccaa gatcctcaag cctgccgccg gatccggttg ggtcgcgcgt
     2881 cgcgtcgaca aggccatgga caccctgaag aacagctatg gcgcgtccct cgacatgctg
     2941 gtcggtagcc gagtagccgt catcggcgtc gccgccatga tcctcgtggt cgctggtgga
     3001 gccgtcggga tgttcttcat cctgcccagc gaactggtgc ctcaggaaga ccggggccgg
     3061 gttcaggtcc gggtccaggg gcccgaaggc gccgggtttg attacaccaa aaagatcatg
     3121 ctcggcctcg agccgattct ggcccagtac aaggccgacg gcgaggtcga gagctatctg
     3181 atctcggcgc ccggcttcgg cggcggcagc tacaactccg gcaacgccac cctgatcctg
     3241 aaggactggt ccgagcgcaa gcgcgccgcc gacgtgatcg cgcaggagat caacggcaag
     3301 ctgcgcggcc agacgggcgc ccaggtcaac gcctccattc ccggcgcctt ccaacgcggc
     3361 ggcggcgggg gaggggggaa ctccaactcg gtcgagatga tcgtcaccgg ttcggagtac
     3421 gaggacatct atcgctggct tcagccggtg ctggcggcct cactggagaa ccccggcttc
     3481 tcgcgtccac gcctgaacta cgagcccaac tcgccgcgtc tgctggtcga tgtcgatccg
     3541 gagaagtcgg ctgcactggg cgtgtcctcg caggaaatcg ggcgtacgct ggagacgatg
     3601 ttcggttcgc gccgcgccac gacctacatc cgtggcggtc aggagtacga cgtcattctg
     3661 caaaccgagc gagccaaccg cgcgggcgtg gctgacctcg aggcgcttta cgtcagcacc
     3721 ggttcgggca cgctcgtgcc gctggcctcg gtcgtggaca ccaagacgtc cggcgacact
     3781 ccggatcgtc gccgcctcga tcgccagcgg tctatcagcc tgcaggccga cctgaacccg
     3841 ggcgagacca tcgccgacgc cgtagccttc ctcaacgccg aggtcgccaa acagcctcag
     3901 ggcgtcgtgg cgatccagtg gggcggggcg gcgcgagacc aacaggaagc cggcggcgcg
     3961 gtcggtgcgg ccttcggtct ggcgctgttg ctggtcttcc tcgtgctggc ggcccagttc
     4021 gagagctgga taactccggc ggtcatcatg ctgacggtgc cgcttgcggc ggccggcggt
     4081 ctgttcggcc ttcttatggc tggtagtagc ctgaacatct actcgcagat cgggctgatc
     4141 atcctgatcg gggtcgcggc caagaacggc attctgatcg tggagttcgc caaccagttg
     4201 cgggatcagg gccggtcgat ccgcgaggcc atcatcgaga gttcgacgct gcgtctgcgc
     4261 ccgatcatca tgacctccat cgccacggcg ttcggcgccc tgccgctgat gctgtggcac
     4321 ggcgcggggg cgggcagtcg ccagaccatc ggcgtggtga tcttcaccgg ggcgatgttc
     4381 tcgaccctgc tgacgctgtt cgtggtgcca gtgatctacg gcgcgctcgc acgcttcacc
     4441 acgtcgcccg aatggaccgc ccgcaagatc gaacagtggg aagcgcagga agccaccgcc
     4501 aacgacccgg tgccgcacgc ggcggaatag ccatcaggac ggggcgggtc gagtgtctcc
     4561 agttctgcga aaatgcagga aatggagaca ctggatacag tcggttcgag acaggcatcg
     4621 aacgacgatg ctgagtggag gcctgccctt tagaccgctt tcctgagacg gaggcggcta
     4681 ggctggatgt caaagaccgt gccggcccta aggaggagcc gcagcgtcat tgtacctgtg
     4741 cgggaagggc cgtcggatcg aaactgccaa ataagtccgg gagcaggggg ccggaatcca
     4801 cggagtgtct ccgatggggt ccggagtatc agggtcgagt gtctccagtc tcggcgcccg
     4861 cgcccattgc gagactccta gcgcttggcc ccttccacac tgaggaaggc gatgacgtcg
     4921 cggcggtctt cggcgttggg gaggccgggg aagctcatgc ggttgccggg caggaaggtg
     4981 cggggattag tcagccagtg gtcgatacgg tcgtcgtccc aggtgaagcc cgccgccttg
     5041 agtggctttg agtagttgaa gcctggatgg gtgccggcga cccgaccgat gacgccgtac
     5101 aggttcggcc ccgtcatgtt cggcccgttc ggggtgacgc tgtggcaggc gcggcaacgg
     5161 gcgaagatcc gggcgccgtt ttccagatca gcctgattgt aaggcagggg cagggcggca
     5221 agcatggtcg cgcgctcgac gtcgctaagg ggcggcggaa cagggaccg
//
LOCUS       DASVTM010000055         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_856156,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000055 DASVTM010000000
VERSION     DASVTM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856156"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF633_01410"
     CDS             complement(<1..203)
                     /locus_tag="VF633_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469744.1"
                     /translation="MNTEKDTRPSDTEAAHLPQNGNADGPARIDQNFVHEIEGAERRE
                     RTGEPGIHGGVAGPAAGPEVADRL"
     gene            251..469
                     /locus_tag="VF633_01415"
     CDS             251..469
                     /locus_tag="VF633_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469745.1"
                     /translation="MLIASRYTMKDARTWPAFVTVRGRCVAGAGRRIRLTRSITGADD
                     AGVAVHESSLVSHKQLQFDSVPKEMSRL"
     gene            605..1045
                     /locus_tag="VF633_01420"
     CDS             605..1045
                     /locus_tag="VF633_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8469746.1"
                     /translation="MKVTDCMTRDVRVVSPELPIFEAARLMLETDVGALPVVIDERVQ
                     GMVTDRDIAVCAVAQQMGLDTPVRVIMSGDLIVAYEDEDADDVALRMSDQQVRRMPVL
                     SRDQSLIGMIALADLSRSDDTSTAEEALSGVSEPGGEHNQSAQA"
     gene            1399..1776
                     /locus_tag="VF633_01425"
     CDS             1399..1776
                     /locus_tag="VF633_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8469747.1"
                     /translation="MGHPGLRVLLVEDEFLQADDLSSALTDQGARVVGPASSIIEALD
                     LLDADDRVDAAVIDINLHGDTSMALAYRLMTDNIPFVFLTGYDRSWLTSAFAGIEVWP
                     KPFEFPTLRAWLEKRMDQAEPAS"
BASE COUNT          325 a          627 c          619 g          379 t
ORIGIN      
        1 agcctgtccg cgacctccgg tccggcggcc ggaccggcga cgcctccgtg gatgccgggt
       61 tcgccggtac gctcccgccg ctccgcgcct tcgatttcgt ggacgaaatt ctggtcgatc
      121 cgcgccggcc cgtccgcgtt gccgttctga ggcagatgcg cggcctctgt gtcactgggc
      181 cgggtgtctt tttcggtgtt catagcgtcc tccagacttt tccgctcgtc gccgagccaa
      241 ggcgcgtccc atgctcatcg ccagccggta cacgatgaag gacgcccgaa cctggccggc
      301 tttcgtcacc gtgcgcggcc gatgcgttgc cggcgcaggg cgccgcattc ggctgacccg
      361 ctccatcacg ggtgctgacg atgcaggagt agccgtccat gagagctcct tggtgtcgca
      421 caaacagctg cagttcgact cggttccaaa ggaaatgtct cgattgtaga acggcttaac
      481 aaaggacagg attggtcgat ccttccggtc ttcgtcgtga agccgtcact ccggaacgtc
      541 ggccgcgggt ccgttttgtt ggcggagggc gggcatatgg tccgccgcca aagggacaac
      601 accgatgaaa gtcaccgact gcatgacccg cgacgtgcgc gtggtctcgc cggaactgcc
      661 gatctttgaa gccgcccgac tgatgctgga gaccgatgtc ggcgccctgc ccgtcgtgat
      721 cgacgagagg gtgcagggca tggtcaccga tcgcgacatc gccgtgtgcg ccgtggccca
      781 acagatgggg ttggacacgc ccgtccgtgt catcatgagc ggagacctga tcgtcgccta
      841 tgaagacgag gacgccgacg acgtcgccct gcgtatgagc gaccagcagg tgcgtcggat
      901 gccggttctc agccgtgacc agtccctgat cggaatgatc gcactggccg acctgtcccg
      961 ctccgacgac acctcgaccg ccgaggaagc gctgagcggc gtctccgaac ccggaggcga
     1021 gcataaccag tccgcccagg cctgaggcgt gttccctcgt ccagcggcct cagctcctag
     1081 ctgtcggaat gagatcaggg ttgagctgcc gggtggggcg ggcggtcaga taaggggccg
     1141 cccgctcgtc tcctgggcgc tagacgaggc cgaccgctct cccatgtatt cggacaacaa
     1201 gcccggtcct gcgaagtctc cgggatcacc tggcggcctg agcctggcgt gccgagcaaa
     1261 ggctccagac tggttgggtt tgcgagcgcc cgcgaagata tttgctcacg tttcgtcgga
     1321 ctcacctatg tcggtgcggg cttgccttgg ctgccgctac cgatggcaat catgattgtt
     1381 catctacgga cacactctat gggacaccca ggtctgcgcg tgcttctggt ggaagacgag
     1441 tttctccagg cggacgacct cagttccgcg ctgactgatc aaggagcccg cgtggtcggt
     1501 ccggcgtcct ccatcatcga ggctctggat cttctggacg ccgatgaccg cgtggatgcg
     1561 gcggtgatcg acatcaatct gcatggtgac acctctatgg cgctggccta tcgcctgatg
     1621 accgacaaca tcccgttcgt cttccttacg ggctacgatc gttcgtggct gacatcggcc
     1681 ttcgcgggca tcgaagtctg gccgaagccg ttcgagtttc caacactgcg cgcatggctc
     1741 gaaaagcgga tggatcaggc ggagcctgcc tcctaggcct tggcaccctc aacgaaccgc
     1801 ctgaggcaaa tccgccaaaa tatcggcctt gcccatcgtt ttggagaggc taaggagacg
     1861 cggatcgcct cggctgccgc aacgtctgca ggaacggtcg ggcttcaaga tcgcagccct
     1921 gccggcgatc ccggcaccgt gcggcgcctg
//
LOCUS       DASVTM010000056         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_431165,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000056 DASVTM010000000
VERSION     DASVTM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431165"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VF633_01430"
     CDS             complement(<1..1335)
                     /locus_tag="VF633_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8469748.1"
                     /translation="MPCLTSTIDPNSAAFKTLDAHNRALAAELRDKVAKAALGGAEAS
                     RERHVARGKLLPRDRVERLLDPGSPFLEIGQLAANGMYGDVKDPDGAPGAGMICGVGR
                     VSGREVVIVANDPTVKGGAYFPMTVKKHLRAQEIAEQNNLPCVYLVDSGGANLPHQAE
                     VFPDRDHFGRIFFNQARMSARNIPQIACVMGSCTAGGAYVPAMSDETVIVRNQGTIFL
                     AGPPLVKAATGEVISAEELGGAETHARKSGVVDYVAENDEHALEIVRDIVGHLNSVKT
                     VGMDVREPRAPTLDPEELYGLIPEDVRAPYDVREVIARVVDGSEFEEFKPLYGSSLVC
                     GFARIWGYPVAILANNGVLFSESAQKGAHFIELACKRKIPLVFLQNISGFMVGGKYEA
                     EGIAKHGAKLVTAVASAEVPKFTVLIGGSFGAGNYGMCGRAYSPRFLFTWPNS"
     gene            complement(1335..2498)
                     /locus_tag="VF633_01435"
     CDS             complement(1335..2498)
                     /locus_tag="VF633_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX8469749.1"
                     /translation="MSIPFAPQSMEFGLGENADMIRETTARWAADRLAPRAAEIDEKN
                     EFARDLWPEMGELGLHGITVEEEYGGLGMGYLEHVVAMEEVSRASASIGLSYGAHSNL
                     CVNQIRRWGNAEQKAKYLPKLISGEHLGSLAMSEAGSGSDVMSMRTKAEKKGDRYVLN
                     GTKFWITNSPTADTLVVYARTGEGSGGVTTFLIEKGMKGFSVSKKLDKMGMRGSDTAE
                     LVFEDCEVPEENVMGPIGGGAGILMSGLDYERTVLSGGPLGIMQAALDVVLPYVRERK
                     QFGKAIGSFQLMQAKVADMYVALNSARTYVYAVAKACDAGKTTRYDAAGAILLASENA
                     VKVSLEAVQALGGAGYTKEWPVERLVRDAKLYDIGAGTNEIRRFLIGRELLGS"
     gene            2671..3063
                     /locus_tag="VF633_01440"
     CDS             2671..3063
                     /locus_tag="VF633_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8469750.1"
                     /translation="MDRPIVLIIDDEPLISTMVEATFEDAGFEVRCVLDAAEAAQQIA
                     ELDTRLSAIVTDIRLGPGPNGWALAVDARTKLPLLPIVYMTGDSAADWTAFGVPRSIL
                     VQKPFVGAQVLAAVMTLMNSDNTESPSA"
     gene            complement(3173..>4344)
                     /locus_tag="VF633_01445"
     CDS             complement(3173..>4344)
                     /locus_tag="VF633_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469751.1"
                     /translation="VPDINKFVAAYNIYSNTGIYAISDQSTGARGQNVNVQENDTGAY
                     LQADFRFNAFGIPFRGDAGVRYVLTEQYSNGYASTGGQIQLVSARRDYDTFLPSANLA
                     ADITDNVVARISAAQTISRPSIGSLTPGGDVGIQGGNFTFSSGNPNVLPTKSNNVDFS
                     LEYYPDSDSLFSVGVFYKKFDTFVQTLSQNIPFSSLGLPSSLLVGAGLNGTDLPASTI
                     FRTTQPINTDGGTLKGFEINAQKRLSFLPGWMENFGVQANYTFVSSDIEYLTSTAPGA
                     ATINETLVGLSKNAANATLYYETEKFSIRGSVAYRQGYLTQVPGNDGNSVHGTNETIN
                     FDAQASYQITPQLRASVEGINLTDEYNDQYVDASNRLNVLTHTGRQVFVGLRYTF"
BASE COUNT          760 a         1391 c         1361 g          832 t
ORIGIN      
        1 gctgttcggc caagtgaaca ggaaacgcgg cgaataggcc cgcccgcaca tgccgtagtt
       61 acccgcaccg aacgatccgc cgatcaacac cgtgaacttc ggcacctcgg ctgaggcgac
      121 agcagtgacc agtttggcgc cgtgtttggc gatgccctca gcctcgtatt tgccgccgac
      181 catgaagccc gagatgttct gcaggaacac cagcgggatc ttgcgcttgc aggccagttc
      241 gatgaagtgc gcgcccttct gggcgctctc cgagaacagc acgccgttgt tggccaggat
      301 ggcgaccgga tagccccaga tgcgcgcgaa accgcagacc aggctcgatc catacagcgg
      361 cttgaactcc tcgaactccg agccgtccac gacccgcgcg atgacctcac gcacgtcata
      421 gggcgcacgc acgtcctcgg ggatcaggcc gtacagttcc tcgggatcca acgttggcgc
      481 acggggctcg cgcacatcca tcccgaccgt cttgacgctg ttcaggtggc cgacaatgtc
      541 gcgcacgatc tccagcgcat gctcgtcgtt ctcggcgaca tagtcgacga cgccggactt
      601 gcgtgcatgg gtctcggccc cgcccagttc ctcggccgag atgacctcgc ccgtcgcggc
      661 cttcaccagc ggcggcccgg cgaggaagat ggtgccctga ttgcgcacga tcaccgtctc
      721 gtccgacatc gcaggcacat aggcgccacc cgcggtgcac gatcccatga cgcaggcgat
      781 ctgcgggata ttgcgcgcgc tcatccgcgc ctgattgaag aagatgcggc cgaaatggtc
      841 gcggtcggga aacacctcgg cctgatgcgg caggttggcc ccgccgctgt cgaccagata
      901 gacgcatggc aggttgttct gttcggcgat ctcctgcgcc cgcagatgct tcttcaccgt
      961 catcgggaag tatgcgccgc ccttgaccgt cgggtcgttg gccacgatca cgacctcacg
     1021 gcccgacacc cggccaaccc cgcagatcat tcccgccccc ggcgcgccgt cagggtcttt
     1081 cacgtcgccg tacatgccgt tggccgccag ttgtccgatc tcaaggaacg gcgagccggg
     1141 atccagcagc cgttcgaccc ggtctctagg cagcagcttg ccgcgcgcca catgccgctc
     1201 ccgcgacgcc tccgccccac cgagtgccgc cttggccacc ttatcccgca gctcagcggc
     1261 cagggcgcga ttgtgggcgt ccagcgtctt gaacgccgcg ctgttcggat caatcgtgga
     1321 ggtcagacag ggcatcagct tcccagtagt tcgcggccga tcaggaagcg ccggatctcg
     1381 ttcgtgcccg cgccgatgtc gtagagcttg gcgtcgcgga ccaatcgctc gaccggccat
     1441 tccttggtgt agccggcgcc gcccagcgcc tgcaccgcct ccagcgacac cttcaccgcg
     1501 ttttcggacg ccagcaggat cgcgcctgcc gcgtcatacc gcgtggtctt gcccgcgtcg
     1561 caggccttgg caaccgcata gacataggtc cgggccgagt tcagggcgac gtacatgtcg
     1621 gcgaccttgg cctgcatcaa ctggaacgag ccgatggcct tgccgaactg cttgcgctcg
     1681 cggacgtagg gcaacaccac atccaaggcc gcctgcatga tgcccagcgg gccgcccgac
     1741 aacaccgtcc gctcatagtc caggccgctc atcaggatac cggcgccgcc gccgatcggg
     1801 cccatgacgt tctcctcggg aacctcgcag tcctcgaaca ccagctcggc ggtgtccgac
     1861 ccgcgcatgc ccatcttgtc cagcttcttc gagacgctga accccttcat ccccttctcg
     1921 atcaggaaag tggtgacccc gcccgagccc tcgcccgtgc gggcatagac caccagggtg
     1981 tcggcggtcg gggagttggt gatccagaat ttcgtgccgt tcaggacgta gcgatcgccc
     2041 ttcttctcgg ccttggtgcg catcgacatg acgtcggagc cagagcccgc ttccgacatg
     2101 gccagcgacc ccagatgctc gccggaaatc agcttcggca ggtacttagc cttctgctcc
     2161 gcgttgcccc agcggcggat ttgattgacg cacaggttgg agtgagcgcc gtagctcagg
     2221 ccgatcgagg ccgaggcgcg tgacacttct tccatcgcca cgacgtgttc gaggtagccc
     2281 atgccgaggc cgccgtattc ttcctcgacg gtgatgccat gcaggcccag ctcgcccatc
     2341 tcgggccaca ggtcgcgagc gaactcgttc ttctcgtcga tctcggcggc gcgaggcgcc
     2401 agccgatcgg cggcccagcg cgccgtggtc tcgcggatca tgtcggcgtt ctcgccgaga
     2461 ccgaactcca tactttgggg cgcgaagggg atactcatga tgtttcctcg atcctgggac
     2521 gcctcttggg gagtctccga cgccccctta gccgccatcg cgatacttgg gaatttgaat
     2581 tgctggtgga cggatccaga atgtcagcta gcgtacggaa cccgccccgg tttcatgctt
     2641 ttgcctcgca gcggggagcg tagactgact atggacagac caatcgtact catcatcgat
     2701 gatgagcccc tgatttcgac catggtcgaa gcgaccttcg aggacgctgg cttcgaggtt
     2761 cgctgcgttc tcgatgcggc cgaggcggca cagcagatcg ctgaactgga cacccggtta
     2821 tcggccatcg ttaccgacat tcgactgggc cctggtccaa acggttgggc gctagccgtg
     2881 gatgcccgca ccaaactgcc gcttttgccc attgtctata tgaccggtga cagcgccgcc
     2941 gattggacgg ccttcggagt gccccggagc atactggtcc agaagccgtt cgtcggcgcc
     3001 caggttctgg cagccgtgat gacgctgatg aactctgaca acaccgaaag cccgtccgca
     3061 taactggcct cgtcgcatcg gggcgatcag cggcgttggg atggcaaaaa aacgccggat
     3121 ccacgaatgg atccggcgca gggactaacg ttcgggagga gcgtcagaag ggttagaagg
     3181 tgtagcgaag gcctacaaag acctgacgac cagtatgggt cagcacgttc aggcggttcg
     3241 aggcgtcgac gtactggtcg ttgtactcgt cggtcaggtt gatgccctcg acgctggccc
     3301 tcagttgcgg tgtgatctgg tacgaggctt gggcgtcaaa attgatcgtc tcgttggtgc
     3361 cgtgaaccga gttgccgtcg ttgccgggaa cctgggtcag gtagccttgg cgataggcca
     3421 ctgaaccgcg gatcgagaat ttctcggtct cgtagtacaa ggtggcgttg gccgcgttct
     3481 tggacagtcc gaccagcgtc tcgttgatcg tcgcggcgcc gggggcggtc gaggtcaggt
     3541 actcgatgtc cgaactgacg aaggtgtagt tggcctgcac cccgaagttt tccatccagc
     3601 ccggcaggaa gctaaggcgc ttctgagcgt tgatttcgaa gcccttcagc gtgccgccgt
     3661 ccgtgttgat cggctgcgtg gtgcggaaga tcgtgctggc tggcagatcg gttccgttca
     3721 ggcccgctcc aaccagcaac gacgacggca ggccgagcga gctgaacggg atgttctggc
     3781 tgagcgtctg aacgaaggtg tcgaacttct tgtagaagac gccgaccgag aacaggctgt
     3841 cgctgtccgg atagtactcc agcgagaagt ccacgttgtt ggacttggta ggcaggacgt
     3901 tcggattgcc cgagctgaag gtgaagttcc cgccctggat cccgacgtcg ccgcccggcg
     3961 tcaggctgcc gatcgaagga cgcgagatgg tctgggccgc gctgatgcga gcgacgacat
     4021 tgtccgtgat gtcggccgcg aggttcgccg agggcaggaa cgtatcgtag tcgcggcgtg
     4081 cagataccag ctggatctga ccaccggtcg aggcgtagcc gttggagtat tgctccgtca
     4141 ggacgtagcg gacacccgcg tcaccccgga acggaatgcc gaaggcgttg aagcggaagt
     4201 cggcctgcag ataggcccct gtgtcatttt cctggacgtt gacgttctgg ccgcgcgcgc
     4261 cggtcgattg gtcgctgatc gcgtagatgc cggtattcga gtagatatta taggctgcga
     4321 cgaacttgtt gatgtctggg acaa
//
LOCUS       DASVTM010000057         4558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_5964, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000057 DASVTM010000000
VERSION     DASVTM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5964"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2371
                     /locus_tag="VF633_01450"
     CDS             <1..2371
                     /locus_tag="VF633_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8469752.1"
                     /translation="TIAANMRTEGLLTQSQLLTAELQAQQGELKTTNDRLEQQAASLR
                     QSEELLRAKQEELQQSNAELEDKAVLLSTQNKQVEAKNQEVEQARFALEEKAEQLALT
                     SKYKSEFLANMSHELRTPLNSLLILSKMLSENTQGNLTLKQVEFAKNIHAAGSDLLGL
                     INDILDLSKIESGTVTLDVSDTPFASLREQMQRTFAQLAQDKKLDFSIDIDPSLPASI
                     YTDDKRLRQVIKNLLSNAFKFTAAGSVTFRVARVATGWTASNEHLATAEGVVAFSVQD
                     SGIGIPEDKQRIIFEAFQQADGTTSRKYGGTGLGLSISREITRTLGGELKVESEPDHG
                     SVFTLYLPLAFSPALAQAPSSRDPLNKAVTVRSVPAERFEDAPEPISDDRDSLTPDDA
                     VVLIVEDDPRFGSILLALAHDCGFKGVITREGVAAASLALRYAPQAIMLDVGLPDMDG
                     LALLDLLKRTPETRHIPVHVISADDQAALGLTIGAFGFTAKPVDREGVIASLEGVRRF
                     VSAPDRRLVLVGRSDAATATLEAGFGSVVHADRLDDLGDILASASDSLVVSADAAPVS
                     ELLDYLKTAERPAIVYADRPLGEEDERRLRFASFSGYTRVARTPDQLVEQVTLMLHEP
                     TDRLSDSARATLAQTRKEDAILTGRTVLVIDDDIRNIFSMASALEEFGVALHYAESGR
                     AGIDLLRQHPEVDIVLVDIMMPDMDGYETMREIRSLTAFTNLPMIAVTAKAMKGDRLK
                     CIQAGASDYVSKPVDIDYLISVMRVAIQRTDALRFGVQSLSGAVEPVDA"
     gene            2391..4403
                     /locus_tag="VF633_01455"
     CDS             2391..4403
                     /locus_tag="VF633_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8469753.1"
                     /translation="MLSAPLVAKVLIVDDDERNAFAATEALATLGHELIVARSGEEAL
                     RSLLTNEFAVILLDLHMPGMDGYETARLIREHPKTRDTPIVFVTAVFRDDAHIFQAYS
                     VGAVDVVFKPADPFILRSKVAILVDLYLKTQEIRRQADESARLLRENARVSAEKSEAE
                     AALRRSRARQDAILQSLPIVFTSRAPTYPFGALFVSDSITPITGFEPVRFVEEADLGL
                     GRIHPDDVSAVADCLLGASETGAYACEYRWLCADGSYRVFLDQGVLAPRHDGDGMEII
                     GTILDVTDRRSLEEQLTQARKMEAVGQLTGGVAHDFNNLLTVVLGNADILLRRGLADD
                     KQARQLGAIRQAAERGQTLTRQLLAFSRRQTLNPVVIELNALVRDFAPLLQQAVGEGV
                     ALEILLSDEAICAHVDPTHLETALLNLSINARDAMAREGRLTIQVRAEPAPGNGDGDG
                     DDRVLITVSDTGAGMAADVANRIFEPFYTTKEVGKGSGLGLSQVYGFVTQSGGEISVE
                     TQPGQGAAFTLSLPRSPQGPQERTATEAHPIAPASERLLIVEDDPDVLNLCVDLLEGL
                     GYQCDTATNAGEALVRLDTCGPYDLMFSDVVMPGGMTGIELARRASQTFPTMRVLLTS
                     GYVGENALQDTHAFDVIDKPYEQASLARRLRLLLDHETRKKLAAVP"
BASE COUNT          841 a         1484 c         1402 g          831 t
ORIGIN      
        1 caccatcgcg gccaatatgc gcaccgaggg gctgctgacc cagtcccagc tcctgaccgc
       61 cgaattgcag gcccagcagg gcgaactcaa gaccactaac gaccggctgg aacagcaggc
      121 cgcctcgctg cgtcagtccg aggaactgct gcgcgccaaa caggaagaac tccagcagtc
      181 caacgccgag cttgaggaca aggcggtgct gctctccacc cagaacaagc aggtggaggc
      241 gaagaatcag gaggtcgaac aggcccggtt cgccctcgag gaaaaggccg aacagctggc
      301 gctgacctcg aaatacaagt ccgagttcct ggccaatatg agccacgaac tgcgcacacc
      361 gctgaacagc ctgctgatcc tgtccaagat gctcagcgag aacacgcaag gcaacctcac
      421 gctcaaacag gtcgagttcg ccaagaacat ccacgccgca ggctcggatc tgctgggcct
      481 gatcaacgac attctcgacc tctccaagat cgaatccggc accgtaacgc ttgatgtcag
      541 cgataccccg ttcgcgtcac tgcgcgagca gatgcaacgg accttcgccc agttggcgca
      601 ggacaagaag ctcgacttct ccatcgacat cgacccctcg ctgcccgcat cgatttacac
      661 ggacgacaag cgtctccggc aggtgatcaa gaacctgctg tcgaacgcct tcaagttcac
      721 cgctgctggc tcggtgacgt tccgtgtcgc gcgggtcgcc accggctgga cggccagcaa
      781 cgaacacctt gcaaccgctg agggggtcgt ggccttctcg gtgcaggaca gcggcatagg
      841 catccctgag gacaagcagc gcatcatctt cgaggccttc cagcaggccg atggcacgac
      901 gagtcgcaaa tacggaggca cgggcctcgg actgtcgatc agtcgcgaga tcacccgtac
      961 gctgggcggg gagttgaagg tcgaaagcga gccggatcac ggcagtgtat tcacgcttta
     1021 tctgccattg gccttcagtc ccgccctggc ccaggcgccg tcttcgcgcg accctctcaa
     1081 caaggcagtt actgtccgca gcgtccctgc cgaacggttc gaggatgctc cggagcccat
     1141 cagcgacgac cgggacagcc ttacacccga cgatgcagtg gtcctgatcg tggaggacga
     1201 cccccggttc ggatcgatcc tgctggcttt ggcccatgac tgcggcttca agggggtcat
     1261 cacgcgtgag ggcgtcgcgg ccgcttcact ggccctgcgt tatgctcccc aggccatcat
     1321 gcttgacgtc ggcctcccgg acatggacgg gctggcgctt ctggatcttc tgaaacgaac
     1381 cccggagacc aggcatattc ccgtccacgt catttccgcc gacgatcagg ccgctctagg
     1441 cctgacgatc ggagctttcg ggttcaccgc caagcccgtg gatcgggagg gcgtgatcgc
     1501 cagcctggag ggcgtgcgcc ggttcgtttc tgcgcccgac cgcaggcttg ttctggtggg
     1561 acgatcagac gcggcgaccg cgacgcttga agccggcttc ggatcggttg ttcacgctga
     1621 tcggctggac gatcttggcg acatcctcgc ttctgccagt gacagtctcg tggtgtcggc
     1681 ggacgcggcg cctgtcagtg agttgctcga ctatctgaaa acggccgaac gacctgcgat
     1741 cgtctacgct gatcgtcctc tgggcgaaga ggacgagcgt cgcctgaggt ttgccagctt
     1801 ctcgggctac acccgtgtcg cacggacccc tgaccagttg gtcgaacagg tcaccctgat
     1861 gttgcacgag ccgacggacc gcctttcgga ctcggcccgc gcgaccctcg cccagacccg
     1921 caaggaagat gcgattctga ccggccgtac ggtgctggtg atcgacgacg atatccggaa
     1981 catcttctct atggccagcg cgctggagga gttcggcgtc gcgctccatt atgccgaaag
     2041 cggccgggcg ggcatcgatc tgcttcgcca gcatccggag gtcgatatcg tacttgtcga
     2101 catcatgatg cccgacatgg atgggtacga aacgatgagg gagatacgct ccctgaccgc
     2161 cttcacgaac ttgccgatga tcgccgtcac ggccaaggcc atgaagggcg accggttgaa
     2221 gtgtatccag gccggtgctt cggattacgt ctccaagcct gtcgatatcg actacctcat
     2281 ctcggtcatg cgggtggcca tacagcgcac tgacgccctc cgtttcggtg ttcagtcact
     2341 gtccggcgcg gtcgagccgg tggacgccta gcgtgggtgg gcactccacg atgctttccg
     2401 cgccgctggt cgccaaggtt ctgattgtcg atgacgacga acggaacgct ttcgccgcga
     2461 ccgaggccct ggccacactc ggacacgagt tgatcgtcgc ccgctcgggc gaggaggcct
     2521 tgcgcagcct gctcaccaac gagttcgcgg tcattctcct cgaccttcac atgcctggca
     2581 tggacggtta cgagaccgcg cgtctgatcc gcgaacatcc caagacacgt gacaccccga
     2641 tcgtgttcgt cacggcggta ttccgcgatg acgcccacat tttccaggct tactccgtgg
     2701 gcgccgtcga cgttgtcttc aaaccggcgg atccattcat cctccgctcc aaggtcgcaa
     2761 tcctggtcga tctgtatctt aagacgcagg agatccgccg acaggccgac gagagcgcga
     2821 ggttgcttcg ggagaatgcc cgggtcagtg ccgagaagag cgaagcggaa gcagcgcttc
     2881 ggcgcagcag ggcccggcag gacgcgatcc tccagtcact tccgatcgtg tttacctcaa
     2941 gggcgccgac ctatcccttc ggggccctgt ttgtcagcga cagtattacg ccgatcacgg
     3001 gattcgagcc ggttcgcttc gttgaggagg ctgatctggg tcttggccgc attcaccccg
     3061 atgatgtctc tgccgtggcg gactgtctgt tgggcgcatc ggagacgggc gcctacgcct
     3121 gtgagtatcg ttggctatgt gccgacggaa gctacagggt ctttctcgac caaggcgttc
     3181 tggcgcctcg tcacgacggt gacgggatgg aaatcatcgg caccatcctt gacgtgaccg
     3241 accgccggtc tctcgaggaa caacttaccc aggcccgcaa gatggaagct gtggggcagc
     3301 tgaccggcgg tgtggcccac gacttcaaca atctgcttac cgtcgttctg ggcaacgccg
     3361 acatccttct gagacgcggc ctcgccgatg acaaacaggc gcgccagctc ggtgcgatcc
     3421 ggcaagccgc cgaacggggg caaaccctga cccgccagtt gctggctttc tcgcggcgtc
     3481 agaccctcaa tcccgtggtc atcgagctca acgcgctggt ccgcgacttc gcccctttgc
     3541 tgcagcaggc ggtcggcgag ggcgtggctc tggagatcct cctgagcgac gaggcgattt
     3601 gcgcccacgt cgatccaacc cacctcgaga ccgcgcttct caacctttcg atcaatgccc
     3661 gggacgccat ggccagggag ggacgactga ccattcaggt cagggcggag ccggcgcccg
     3721 gcaacggtga cggtgacggt gacgaccggg ttctgatcac cgtgtcagat accggcgcgg
     3781 ggatggctgc ggatgtggcg aaccggattt tcgaaccctt ctatacgacc aaggaggtcg
     3841 gcaaggggtc cgggctcggc ctgtcccaag tctatggctt cgtcacgcaa tcaggcggcg
     3901 agatctcggt ggaaacgcag cctggtcagg gcgccgcctt caccctcagt ctcccccgca
     3961 gtccgcaggg cccccaggaa cgaacggcga cagaggcgca tccgatcgcg cccgcgtcgg
     4021 agcgcctgct catcgtagag gacgatcccg acgtcctgaa cctctgcgtc gatcttctcg
     4081 agggtctcgg ctatcagtgc gacacagcga ccaatgcggg cgaggcgctg gtccggctcg
     4141 atacctgtgg gccctacgat ctgatgtttt cggatgtcgt catgccgggc gggatgaccg
     4201 ggatagaact ggcccggcgc gcgtcccaga ccttcccgac aatgcgggtt ctgctgacgt
     4261 ccggctatgt cggcgaaaac gcgctacagg acacgcacgc cttcgatgtg atcgacaagc
     4321 cttacgaaca ggcgagcctt gcccgccggc tgcgcctctt gcttgaccac gagacgagga
     4381 aaaagctggc ggccgttccg tgacggacca cagaccgatc gctggtgcgc ggcacgacgc
     4441 tgggccgggc gcggcgtaga gcgtcgcgag tcccggtgca cggcctccgc tctgcgcatc
     4501 gacctccaca agcggggctc tttcatcctc gaggccacca gtcccgcgcc gcccccct
//
LOCUS       DASVTM010000058         4614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_219116,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000058 DASVTM010000000
VERSION     DASVTM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219116"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VF633_01460"
     CDS             complement(<1..513)
                     /locus_tag="VF633_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8469754.1"
                     /translation="MSDLETFRAETRAWLEANCPAEVRGPLDNDEDRVWGGRNATFRT
                     PAHKLWMERMGARGWTAPEWPSEYGGGGLTREEAKVLGSELRRIDAQMALSSFGIWML
                     GPALLQFGTEEQKKRFLPEIVRGEIRWCQGYSEPGAGSDLAGVQTRAEDPSLDGQGDH
                     WIVNGQKVWTS"
     gene            755..1132
                     /locus_tag="VF633_01465"
     CDS             755..1132
                     /locus_tag="VF633_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469755.1"
                     /translation="MSSIMTSSTRRSRDHFEPQDSDPQEVRRLRGLLEQIDYTAFISN
                     REVIGASLSVVDASTFQRLAVTVATARTKWVSEALRLSESGSPVTPDQTARLTAHRTA
                     FEELSEAYEGLRRMVERSYITLP"
     gene            complement(1154..1495)
                     /gene="fdxA"
                     /locus_tag="VF633_01470"
     CDS             complement(1154..1495)
                     /gene="fdxA"
                     /locus_tag="VF633_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX8469756.1"
                     /translation="MTYIVTDACVKCKFMDCVEVCPVDCFYEGENFLVIAPDECIDCG
                     VCEPECPIDAIKPDTEDEPDGKWLQVNASYAKVWPNITVKGTPPADREEWERVENKFE
                     NHFSPNPGKGS"
     gene            1697..2326
                     /locus_tag="VF633_01475"
     CDS             1697..2326
                     /locus_tag="VF633_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8469757.1"
                     /translation="MINRRFLVRAGAAGALALTLIGGGVVAQAAMTQNPAEVQAGTYK
                     LDPSHGKITWSLKHLGFSTYYGQFTEVTADLTIDPANPSAGTLTATIPMSGLAPNNDA
                     LKTHLLSADFFNAAEFPQATFKSTSITVDADDKSEADVAGELTLHGVTRPVTLEVKFN
                     QAGPAMGGGYKVGFDGEATIKRSDFGITTYLPALGDDVTLHIEGEFLKQ"
     gene            complement(2316..2645)
                     /locus_tag="VF633_01480"
     CDS             complement(2316..2645)
                     /locus_tag="VF633_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX8469758.1"
                     /translation="MAETALSVRIDVWLWRARFAKTRSLAAALVERGAVRLTHQGRQT
                     RLDKPSRLVHPGDEMTFAVDGRVRAVRVEAMGERRGPPAEARALYSSLDASPDPGPEE
                     PDQTTTA"
     gene            complement(2638..>4614)
                     /locus_tag="VF633_01485"
     CDS             complement(2638..>4614)
                     /locus_tag="VF633_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX8469759.1"
                     /translation="AFSTDQVYAIAELIRRQRGGAAVVMGSLSPRTRNAQVALFQSGE
                     VDFLVATDAIGMGLNMDVDHVAFAGMRKFDGRRTRWLHANEIAQIAGRAGRHVRDGTF
                     GVTGECDDLDEDLVEQVVNHQFEPVQAAEWRNARLDFDTLPDLLRSLTKTPERAGLKL
                     TAQSLDETLLRRALQDEEIARISRSRGTIMRLWECCQLPDFQKTTLDEHVRLSRDIFH
                     ALTGKRGRLTDEWFAPRFAELDRDDGQIDQLSARLAGVRTLSYIAARPDWLDQAKTWR
                     EKTRALEDRLSDVLHERLTARFVDRKTTALMRSLNDHAGSLAEVAADGIVTVDGEAVG
                     HLEGVRFAGESGGSLLADRTLKAAALRAVGPEIARRLGKLAADGDDAFSVTPEGEVMW
                     SGALAAKILNAEPFSPRVRLIGDLGPQAARDRAQRRIEAWLASEAGRALRDLRRLKQA
                     IETGGLKGLPRGIAFRLLEAGGILDRRDVERDLAALSQVERRTLKTFAIRVGVHSVWL
                     PGAMKPRARTMTQAFSAGEPFRASGEGLTLLPIPTPSVRALSAFGRRAVGRWSVPVEL
                     LEKLAEAKQAAGKGAIPDATLSHLGLKADEAGALLSALKTVRAQQPDRPGGSPVVIKD
                     SPFAKLAELTASVVPARRKRPRRKPQVGGAASNG"
BASE COUNT          788 a         1639 c         1387 g          800 t
ORIGIN      
        1 ggaggtccag accttctggc cgttgacgat ccaatggtcg ccctgcccat caagactggg
       61 gtcctctgca cgggtctgaa cgccagccag atcggacccc gcgccgggtt cggaataccc
      121 ttggcaccag cgaatttcgc cgcgcacgat ctcgggcagg aaacgcttct tctgctcctc
      181 agtgccgaac tgcagcagtg cgggccccaa catccagata ccgaagctgg acagagccat
      241 ctgggcgtcg atgcggcgca gttccgagcc gagcaccttg gcctcttcgc gggtcagtcc
      301 cccgccgccg tattcgctgg gccattccgg agcggtccag ccgcgcgcac ccatccgctc
      361 catccagagc ttgtgagcag gagtcctgaa ggtggcgttg cgcccgcccc agacgcggtc
      421 ttcgtcattg tcgagcggac ccctgacttc agccgggcaa ttcgcctcaa gccaggcgcg
      481 ggtctccgca cggaaggttt ccagatcgct catgccgtct cccaaacgtc taaccctcgg
      541 cccgcgtgtt cccgcgttcg cctgaagcaa gagttagcag ggtttcgcga tgacgtagcg
      601 tcagtgtttg gtgagaatgg cgccccgcaa ggcatcattg gtcagggctt ggtcactgga
      661 accgatcatt aaccgacttg gcgcaccctc ttgatgacca agcgccagcc ctcccccgag
      721 gaagtcgccc tgagacgggc cagaggatga tccgatgtcg agcatcatga cctccagcac
      781 gcgccgctcg cgcgaccatt tcgaacccca ggacagcgat cctcaggagg tccggcgcct
      841 gcgcggcctg ctggagcaga ttgactacac ggccttcatc tccaatcgcg aagtgatcgg
      901 cgcgtcccta tcggtcgtcg acgcctctac attccagcgg ctggcggtca cggtggccac
      961 agccagaacg aaatgggtgt cggaggcgct gcgactttca gagtccggct cgcctgtcac
     1021 gcctgaccag accgcgcgcc tgacggccca tcgcacggcg ttcgaagaat tgtccgaggc
     1081 ctacgagggc ctgcgccgga tggtcgagcg cagctacatc accctgccgt aagcgcaggc
     1141 aaacgtcaga agatcaagat cccttgcccg ggttggggct gaagtggttc tcgaacttgt
     1201 tctcgacccg ctcccactcc tcgcgatcgg cggggggcgt gcccttgacg gtgatgttgg
     1261 gccagacctt ggcgtacgac gcattgacct gcagccattt gccgtcgggt tcgtcttcgg
     1321 tgtcgggctt gatcgcgtcg atcggacatt ccggctcgca cacgccgcaa tcgatgcatt
     1381 cgtcgggtgc gatgacgagg aagttctcgc cctcgtagaa gcagtccacc ggacagactt
     1441 cgacacagtc catgaactta catttcacgc aggcgtcggt gacgatgtag gtcatgggcg
     1501 gagaggatca cgtgaggcgg gtgaagaacg gtccggggat gtggccccga cagtcggggg
     1561 tgtcaaccga acgcttctat cgacattgaa cagcatggct ttcttgccgc accggccttg
     1621 cagcgttaga tcaaaatgct atttgcacct gcatcgatta cagttttagc ttcacctttc
     1681 cattcggagt cgttccatga ttaaccgccg ctttctcgtt cgcgctggcg ccgctggcgc
     1741 gctggccctc accctcatcg gcggtggcgt cgtcgcccag gccgccatga cgcagaaccc
     1801 cgccgaggtt caggccggga cctacaagct ggatccatcg cacggcaaga tcacctggtc
     1861 gctgaaacac ctcggcttct cgacctacta cggccagttc accgaggtga cggctgacct
     1921 gaccatcgat cccgccaacc cttcggccgg tacgttgacg gcgaccatcc caatgtccgg
     1981 cctcgcgccc aacaacgacg cgctgaagac gcacctgctg tcggctgact tcttcaatgc
     2041 cgccgagttt ccgcaagcga ccttcaagtc gacctcgatc accgttgacg ccgacgacaa
     2101 gagcgaagcc gatgtcgccg gcgaactaac cctgcacggc gtaacccgtc cggtgacgct
     2161 ggaagtgaag ttcaatcagg ccggaccagc aatgggcggc ggctacaagg tcgggttcga
     2221 cggcgaggcc acgatcaagc gttcggactt cggcatcacc acctacctgc cggcgctggg
     2281 cgatgacgtc acgctgcaca tcgagggcga gttcctcaag cagtagtcgt ctgatccggc
     2341 tcttcggggc cagggtcagg actagcatcc agactcgaat agagggcgcg ggcctccgcc
     2401 ggcggtccgc gccgctctcc catcgcctcc acgcgaacag cccgcacgcg accgtccaca
     2461 gcgaaggtca tctcgtcgcc tgggtggaca aggcgactgg gcttgtcgag ccgggtctgc
     2521 cgcccctgat gcgtcaggcg aaccgcccca cgctccacca gagccgccgc caacgagcgg
     2581 gtcttcgcga accgggcccg ccacaaccac acatcgatac gaacagacag ggccgtctca
     2641 gccattcgac gccgctcccc ccacctgcgg cttgcgacgc ggccgcttgc gccgtgcggg
     2701 gacgaccgaa gccgtcagct cggccagctt ggcgaatggc gaatccttga tcaccacggg
     2761 actgccgccc ggtcggtcag gctgctgagc gcggaccgtc ttcaaagcgc tcagcagagc
     2821 cccggcttcg tccgctttca gtccgagatg gctgagcgtt gcgtccggga tcgcgccctt
     2881 gcccgccgcc tgtttggcct cggccagctt ctccagcagt tctacaggca cggaccaccg
     2941 tcccactgcg cgccgcccga acgccgaaag ggcccgcacc gatggcgtcg ggatcggcaa
     3001 cagggtcagc ccctccccgc tcgcccggaa cggttcaccc gctgaaaatg cctgcgtcat
     3061 ggtccgcgcc cgtggcttca tggcccccgg cagccagacc gaatgcaccc cgacccggat
     3121 ggcgaaggtc tttagcgtgc gtcgctcgac ctggctcagc gcagccaggt cgcgctcgac
     3181 gtcgcgtcgg tcaaggattc cacccgcctc caacaggcga aatgcgatac cgcgcggcaa
     3241 acccttcagc ccgcccgtct cgatcgcctg cttgagccgc cgcagatcgc gcaacgccct
     3301 gcccgcttcc gacgccagcc atgcctcgat gcgccgctgc gcccggtccc gcgcggcctg
     3361 aggccccaga tcgccgatca acctgacgcg cgggctgaac ggctcggcgt tgaggatctt
     3421 cgccgccagc gcacccgacc acatgacctc gccctccggc gttaccgaga aggcgtcgtc
     3481 gccatccgcc gccagcttgc ccaaccgtcg cgcgatttcc ggtccgaccg cgcgcagggc
     3541 tgcagccttc agcgtccggt cggccagcaa cgacccgccg ctttcgcccg cgaaccgcac
     3601 gccctccaga tgtccgaccg cctcgccatc taccgtgacg atcccatcgg cggcgacttc
     3661 ggccagcgat ccggcgtggt cgttcagcga ccgcatcaga gccgtcgtct tgcgatccac
     3721 aaaccgcgcc gtcagccgtt cgtgaagcac gtccgacagc cggtcttcca gcgcgcgcgt
     3781 cttctcgcgc cacgtcttcg cctgatccag ccagtcgggc cgggcggcga tgtagctgag
     3841 cgtgcgcact ccggcgagcc gtgccgacag ctggtcgatc tggccgtcat cgcggtccag
     3901 ttcggcaaag cgcggcgcga accattcgtc ggtcagccgc cctcgcttgc cggttagcgc
     3961 atggaatata tcgcgcgaca gccgcacatg ctcgtccagc gtcgtcttct ggaagtccgg
     4021 cagctggcag cattcccaga gccgcataat cgtcccgcgc gaccggctga tccgcgcaat
     4081 ctcttcgtcc tgcagagcac gtcgcagcag ggtctcgtcc agcgactggg cggtcagctt
     4141 cagcccggcc cgctccggcg tcttcgtcag cgaccgcagc aggtccggca gggtgtcgaa
     4201 atccagccgg gcgttgcgcc actcggcggc ctgcaccggc tcgaactgat ggttgaccac
     4261 ctgctcgacc agatcctcat cgaggtcgtc gcattcgccg gtcacgccga aggtgccgtc
     4321 gcgcacatgc cgtcccgctc gcccggcgat ctgggcgatc tcgttagcgt gcagccagcg
     4381 cgtccgcctt ccgtcgaact tgcgcatacc ggcgaaggcg acatggtcga cgtccatgtt
     4441 cagccccatg ccgatggcgt cggtagcgac gaggaaatcg acttcgcccg actggaacag
     4501 agccacctgg gcattacggg tgcgtgggga cagcgagccc atcaccaccg ccgccccgcc
     4561 gcgctgacgc cggatcaact cggcgatggc gtagacctga tcggtcgaaa aggc
//
LOCUS       DASVTM010000059         4757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_485529,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000059 DASVTM010000000
VERSION     DASVTM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485529"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF633_01490"
     CDS             complement(<1..187)
                     /locus_tag="VF633_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469760.1"
                     /translation="MVEALHQVGQIVARRVRPLGEESLKHALTEVLAGRIGSFGLQLA
                     PGVDACTESMEQARRQSC"
     gene            complement(303..779)
                     /locus_tag="VF633_01495"
     CDS             complement(303..779)
                     /locus_tag="VF633_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8469761.1"
                     /translation="MSNPAQVIRPPNTLRLKVGGGFGGIDANAIAKAEAALKAMSAQF
                     GQWLQDEITKLDAAQAAIRTEGLNAQTAEGLYFRAHDLKGLGTTYQYPLVTRLAGSLC
                     KLLDDPTKRATAPVRLLNAHIDAIKAVVRDEIQTDDHPTGRILAETLEAEVAAYRT"
     gene            complement(848..1648)
                     /locus_tag="VF633_01500"
     CDS             complement(848..1648)
                     /locus_tag="VF633_01500"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX8469762.1"
                     /translation="MRDAVTETPFPASSLALAFTASERPEAQFARARLAKRYGSVPVG
                     EADIIVALGGDGFMLETLHGDLTRRTPVYGMNRGSVGFLMNDYEEDDLPERIAAAGRA
                     VIHPLQMDAWNEAGEVSSGLAINEVSLLRQTRQSAKLRISIDGKVRLDELTCDGCMVA
                     TPAGSTAYNLSAHGPIIPLDARMLALTPISAFRPRRWRGALLSHEARVTFEILEPDKR
                     PVSAVADGLEVRRVAKVEVRERRDVSLTMLFEAGRSFEERVLLEQFTG"
     gene            complement(1689..2816)
                     /locus_tag="VF633_01505"
     CDS             complement(1689..2816)
                     /locus_tag="VF633_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX8469763.1"
                     /translation="MTAVASLQTFAPSRAPSFSPADLLTLAKSRKSDDRQRLLLGIAA
                     LCDATPTGSEVSPVLTEIFLTLARQAEHDIRKSLAESLAGAEWAPLALVNMLALDEIE
                     IARPIIANSPLLKDQDLLRILVEATIEHHIEVARRPALSGIVADAIIDAGEPATMTAL
                     ASNRTAEISESGLGRLVEQSRRVAALRAPLTRHPRLNDALAHELYGLVGQALRQAIGE
                     RFRVDDAPLAVAVDAAVASAIPRRAPAPGPSQPSLSDREEMERRLVAKLQSAGQLRAG
                     FLIRAVREKRLSLFEHSLAALAGFSVGQVRTAVMGPTPDALFLACSVVGIDRAVFPAV
                     LDELRKLTNGTPGESSSWNSIKLSPSDAAREFRLLVGDIAA"
     gene            2984..3751
                     /locus_tag="VF633_01510"
     CDS             2984..3751
                     /locus_tag="VF633_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8469764.1"
                     /translation="MADGAAPQSLKSRVSEAEWQARVDLAALYRLVALHGWDDMIFTH
                     ISARVPGPEHHFLINPYGWYFEEMTASALVKVDLDGNIVQDTQSFINPAGFTIHSAIH
                     AAREDAHYVLHLHTTAGVGVAAQQEGLLPIGQNACLLQHQVAYHGYEGLALNHDERER
                     LVADLGDKPLMLLRNHGTLAVGQTAAAAWIGMFFLERACAQQVAALSGGREHVLIAPD
                     AAQEETKQQGGGLPFVAALAWPGALRMLDRKSPGYDT"
     gene            3748..4557
                     /locus_tag="VF633_01515"
     CDS             3748..4557
                     /locus_tag="VF633_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469765.1"
                     /translation="MKAAAALLTGAAASLSLLLIPAVATAGAWTQPKGKGQIIVKYED
                     MHADEGFDPEGRRVPLLVERTDVSTGVFVEYGLTDRLTLQLKGDWQDGEDQFVDYQGR
                     GPLEVALVWQAWRNDRGALSVQAGYSAPGEGRNAGYEAPGEGAGDWEVRVSGGWSFNR
                     KKPAGRWRALLSPDRTFVELQFARRVRSGLPDETRADFTVGRHFADNWMVLNQTYAGR
                     TEDGVSQWVQSETSIVRRLGSWSVQAGWRATTTGRETPAGSGPIVGIWHRF"
     gene            complement(4578..4730)
                     /locus_tag="VF633_01520"
     CDS             complement(4578..4730)
                     /locus_tag="VF633_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469766.1"
                     /translation="MAAAFDEFSKLDAISADAENDKGVNGANVAAVLLFWPAAAGTYL
                     NARDAQ"
BASE COUNT          814 a         1498 c         1609 g          836 t
ORIGIN      
        1 cgcaggactg ccgccgcgcc tgttccatgc tttccgtgca ggcgtcgaca cctggcgcca
       61 gttgcaggcc gaaggagccg atacgtccgg cgaggacttc cgtcagcgca tgcttgagcg
      121 attcctcacc cagcggccga acgcgccgcg cgacgatctg gcctacctga tggagcgcct
      181 cgaccatgcg ccgctctcgg ctatgtcgcg gagtgcggct tctgcggctt aacaccgctc
      241 cggatacgaa aacgcccgct cgtcagagcg ggcgtattgg gttttgttac ggattgccct
      301 tgtcaggtgc gataggcggc gacctcggct tccagggttt cggccaggat gcgcccggtc
      361 ggatggtcgt cggtctggat ctcgtcacgc acgacggcct tgatggcgtc gatgtgggca
      421 ttcagcagtc gtaccggagc ggtcgcgcgc ttcgtcggat catccagcag cttgcacaat
      481 gaaccggcca agcgggtgac cagcggatat tgataggtgg tgccgagacc tttgagatcg
      541 tgagcccgga aatacaggcc ttctgccgtc tgggcgttca gcccctcggt gcgaatcgcg
      601 gcctgagcgg cgtcgagctt ggtgatttcg tcctgcaacc attggccgaa ctgggccgac
      661 atggccttga gcgcggcttc ggccttggcg atcgcattag cgtcgatacc gccgaaaccg
      721 ccgccgacct tgaggcgaag cgtgttgggc gggcgaatga cctgggccgg attgctcacg
      781 tgaactcctg tgggcgcata ggggcttcat agcctcatgg cccttaagga acggtgaccg
      841 ggtggtctta accggtgaat tgttccagca atacgcgctc ttcgaacgat cggcccgcct
      901 cgaacagcat ggtcaggcta acgtcgcggc gctcgcgcac ctcgactttc gccacccgcc
      961 gcacttcgag gccatcggcc acggcgctca ccggtcgctt gtctggctcc agaatttcga
     1021 aggtgaccct ggcctcgtgc gagagcaggg ctccgcgcca ccgccgcggg cgaaaggcgc
     1081 tgatcggcgt aagggccagc atccgcgcgt cgaggggaat gatcgggcca tgagcggaca
     1141 gattgtaggc cgtcgatccc gccggagtcg ccaccatgca gccatcgcaa gtcagctcat
     1201 ccagccgtac cttgccgtcg atcgagatgc gcagtttggc gctctgccgc gtctgcctca
     1261 gcaaagagac ttcattgatc gccagtccgc tggagacctc tccggcctcg ttccaggcgt
     1321 ccatctgcag cgggtggatg accgcccgcc cggctgcggc aatccgctct ggcaggtcgt
     1381 cttcctcgta gtcgttcatc aggaagccga ctgatccccg gttcatgcca tacacaggcg
     1441 ttcggcgtgt cagatcgccg tgcagggtct cgagcatgaa gccatcgcca cccagggcga
     1501 cgatgatgtc ggcctcgccg accgggacgg agccatagcg tttggccagc cgcgcgcgcg
     1561 cgaactgcgc ctcgggacgc tcactcgcgg tgaaggccag ggcaaggctg gaagctggga
     1621 agggcgtttc ggtcacggcg tcacgcaagc cgagcggggc gtccaagtca aacccggaaa
     1681 tgaaattgtc aggcggcgat gtcaccgacg agaaggcgga actcgcgagc agcgtcggac
     1741 ggcgacaact tgatggagtt ccagctggag ctttcgcccg gcgtcccgtt ggtcagtttg
     1801 cggagctcgt cgagtacagc cggaaagacc gctcgatcga ttccgacaac gctgcaggcc
     1861 agaaacagcg cgtccggcgt tgggcccatg accgccgtcc ggacttggcc aacgctaaag
     1921 cccgccaagg cggccagaga gtgttcaaac aggctcagtc gcttctcgcg gacggcgcgt
     1981 atcagaaatc cggcacgaag ctggccggcg ctctgcagct tggcgaccag tcggcgctcc
     2041 atttcctcgc ggtccgacag gctcggctgg ctcggccccg gcgcaggggc gcggcggggg
     2101 atagcactgg cgactgcagc gtcgacggcg accgccaggg gcgcgtcatc gacgcggaag
     2161 cgttcgccga tcgcctgccg cagagcctgc ccgacgagtc cgtagagctc gtgggccagg
     2221 gcgtcgttga ggcgcggatg gcgggtcagg ggcgcgcgca gggcggccac tcgccgcgac
     2281 tgctcgacca gtcgcccaag gccgctctcg ctgatctctg ccgtgcggtt ggaggcgagg
     2341 gcggtcatcg ttgcaggttc accggcgtcg atgatggcgt cggcgacgat gccgctcagc
     2401 gccgggcgac gtgcgacctc gatatgatgc tcgatcgtgg cttccaccag gatgcgtagc
     2461 aggtcctgat ccttcaacag cgggctgttg gcgatgatcg gccgggcgat ctcgatttcg
     2521 tcgagcgcca gcatgttgac cagagccaac ggcgcccatt cggctccggc caggctctcg
     2581 gccagcgact tgcggatgtc gtgttcggcc tgacgcgcga gggtcaggaa gatttcggtg
     2641 aggaccggcg acacttcgct cccggttggc gtggcgtcgc acagggcggc aatgcctagc
     2701 agcagacgct ggcggtcatc gctcttgcgg cttttcgcca gcgtcaacag atccgcaggc
     2761 gaaaagctgg gtgcgcgcga gggggcgaag gtttgcagcg atgcgacagc ggtcacggcg
     2821 ggctcccatc ggaacaggtc cgattctgtt cacaagtctg ggttccggga gtgaaaactc
     2881 ggttaacgaa ctctgaacct gccccagggg catgcttgac gggccgacat caggggagga
     2941 cggtggaggc ctacgtccga agaggcgaca ggaaggaaac cccatggccg acggcgccgc
     3001 gccccaaagt ctgaagtccc gtgtatcgga agcagaatgg caggcccggg ttgatctcgc
     3061 cgccctgtac cggctggtcg ccttgcatgg ctgggacgac atgatcttca cccacatctc
     3121 ggcccgcgtg ccgggacccg agcaccattt cctgatcaac ccctacggct ggtatttcga
     3181 ggagatgacg gcgtcggctc tggtcaaggt cgatctggac gggaacatcg ttcaggacac
     3241 ccagagcttc atcaatccgg ccggcttcac catccattcg gccattcacg ccgcgcggga
     3301 ggatgctcac tacgtcctcc atctgcacac cacggcaggc gtcggcgtgg ccgcccaaca
     3361 ggaaggcctg ctgccgatcg gccagaacgc ctgcctgttg cagcatcagg tcgcctatca
     3421 tggctacgaa ggcttggccc tgaaccacga cgagcgcgag cggctggtgg cggaccttgg
     3481 cgacaagccg ctgatgctgc tgcgcaatca cggcacgctg gccgtcggcc agacggctgc
     3541 ggcggcgtgg atcggcatgt tcttcctcga acgcgcctgc gcccagcagg tggcggccct
     3601 gtccggcggg cgcgagcatg tgctgatcgc gccagatgcg gcgcaggaag aaacgaaaca
     3661 gcagggcggc ggactgccat tcgtggcggc actcgcctgg cccggcgccc tgagaatgct
     3721 ggatcgtaaa tcgcccggct acgacacgtg aaagccgccg ctgcgcttct cacgggcgcg
     3781 gcggcatcac tttcgcttct gctaattccg gccgtcgcca cagcgggggc ctggacccag
     3841 ccgaagggca aggggcagat catcgtcaaa tacgaggaca tgcacgccga cgagggcttt
     3901 gacccggaag gtcgccgtgt gcctttgctg gtggagcgaa cggacgtctc tacgggggtg
     3961 ttcgtcgaat atggcctgac cgatcgcctg actcttcagc tcaagggtga ctggcaggac
     4021 ggcgaggatc agttcgtcga ctatcagggc cgaggaccac tcgaagtcgc ccttgtctgg
     4081 caggcctggc ggaacgatcg tggggcgctc agcgtccagg ccggctattc agcgcccggt
     4141 gaaggccgga acgccggcta cgaggcgccc ggcgaggggg ccggggattg ggaagtcagg
     4201 gtttccggcg gctggtcttt caacaggaag aagcctgccg gacgctggcg cgccttgctg
     4261 tctccggacc gtaccttcgt cgaactgcag ttcgcgcgcc gcgtccggag cggtcttcca
     4321 gacgagaccc gggcggattt caccgtcggc cggcatttcg ctgacaactg gatggtgctg
     4381 aaccagacct atgcaggacg gaccgaggac ggcgtgtccc agtgggttca gtccgaaacg
     4441 tccatcgtgc ggcgtttggg atcgtggagc gtgcaggcgg gctggagggc cacgactaca
     4501 gggcgcgaaa cccccgccgg atcgggtccc atcgtcggca tctggcacag attctgatcc
     4561 tgccgatggg cgaccaacta ttgggcgtca cgggcattca ggtaggtacc tgccgctgct
     4621 ggccagaaca gcagcacggc ggcgacattg gcgccgttca cacccttgtc gttctcagcg
     4681 tcagccgaaa tggcgtcgag cttgctgaac tcgtcgaacg cggccgccac gtgcggtcaa
     4741 gtcgcagcgt ttttgta
//
LOCUS       DASVTM010000060         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_219408,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000060 DASVTM010000000
VERSION     DASVTM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219408"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1520
                     /locus_tag="VF633_01525"
     CDS             75..1520
                     /locus_tag="VF633_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8469767.1"
                     /translation="MQNKGSTGPQGFVVQPRRLPKTMASVRVQDFGEIIVKPTTENVE
                     VQAGRCTDCGVPFCQNACPLQQNIPDWLGLAAEDEMREAWRIASATSTMPEICGRICP
                     QDRLCEGSCTLNQSGWEAVTIGSVEAFIGDTAFDNGWVEPIRPTRERGQSVGIVGAGP
                     AGLAAADRLRSAGYAVTIYDRHDRPGGLLIYGIPGFKLEKRVVQRRTDRLSDGGVEFV
                     QNCNVGVDVTLAELRERHDAVLLAMGVYQARALSVPGGGPGDTVPALDFLIHQNRRDL
                     GEVDNDTWHNAKGRKVVVIGGGDTAMDCVRTAIRQGAESVVCLYRRDKDNMPGSAREV
                     LNAEEEGVVFEWLGAPKALMSRDGAVSSVRAARMALSEPEFGKRREIVTVPGGDFDLQ
                     ADLVIEALGFEPEEFAAHHNELSLTQWNTVAVARFGKDGAGFATSLPGVYAAGDAVRG
                     ASLVVWAVRDGQDAAAEIDRYLRTQTEEQAA"
BASE COUNT          271 a          536 c          515 g          279 t
ORIGIN      
        1 gtctaatacc gtgccaaaag cttgatatcg cggaaatgat ttgctgaacg gaccggtttt
       61 ggttttatgc gcgcatgcaa aacaagggat caaccggccc ccagggcttt gtcgtgcaac
      121 cacgtcgtct tcccaagacg atggcttccg ttcgcgtgca ggattttggc gagatcatcg
      181 tcaaaccgac gaccgagaac gtcgaagttc aggccggacg ttgcaccgac tgcggcgtgc
      241 ccttttgcca gaacgcctgc ccgctgcagc agaacatccc cgactggctg ggtctggcgg
      301 cggaagatga aatgcgcgag gcgtggcgga tcgcgtcggc gacttcgact atgcccgaga
      361 tctgcggccg catctgtccg caggaccgcc tgtgcgaagg ctcctgcacg ctgaaccagt
      421 ccggctggga agccgtgacc atcggttcag tcgaggcctt catcggcgat accgcattcg
      481 acaacggctg ggtcgagccg atccgcccaa cgcgtgaacg cggtcagtcc gtaggcattg
      541 tcggcgcggg tcctgccggt ctcgccgccg ccgaccgcct gcgcagcgca ggctatgcgg
      601 tcaccattta tgatcgccat gaccggcccg gcggcctgct gatctatggc atccccggct
      661 tcaagctgga gaagcgcgtc gttcagcgcc gcaccgatcg cctgtccgac ggcggcgtcg
      721 aattcgtcca gaactgcaac gtcggcgtcg acgtcaccct ggcggaactg cgtgagcgtc
      781 acgacgccgt gctgctggcc atgggtgtct atcaggctcg cgctctgtcg gtgcccggcg
      841 gcggtcccgg cgatacggtc ccggcgctcg atttcctgat ccatcagaac cgccgcgatc
      901 tgggcgaggt cgacaatgac acctggcaca acgccaaggg ccgcaaggtc gtggtcatcg
      961 gcggcggcga taccgcgatg gactgcgttc gcaccgccat ccgccagggc gcggagtccg
     1021 tcgtctgcct gtaccgccgc gacaaggaca acatgccggg ctcggcccgc gaggttctga
     1081 acgccgagga agaaggcgtc gtgttcgagt ggctgggcgc gcccaaggcc ctgatgtcgc
     1141 gcgacggtgc ggtctcgtcc gtccgcgccg cccgcatggc cctgtcagag cccgagttcg
     1201 gcaagcggcg tgagatcgtc accgtgcccg gcggcgactt cgacctgcag gccgatctgg
     1261 tcattgaagc gctcgggttt gagcccgaag agttcgccgc ccaccacaac gagctgtccc
     1321 tgacccagtg gaacaccgtg gccgtcgccc gtttcggcaa ggacggcgca ggtttcgcca
     1381 cctccctgcc cggcgtctat gccgccggcg acgccgttcg cggcgcctcg ctggtggtct
     1441 gggccgtccg cgacggtcag gacgccgccg ccgaaatcga ccgttatctg cgtacacaga
     1501 ctgaggagca ggctgcatga cctggctcaa gcaatatgaa accgaccgcg cccgcctgat
     1561 cgccggccac gcctatgatc ccgcgtcaga gaaagacgcc t
//
LOCUS       DASVTM010000061        14046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_431699,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000061 DASVTM010000000
VERSION     DASVTM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431699"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF633_01530"
     CDS             complement(<1..402)
                     /locus_tag="VF633_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469768.1"
                     /translation="MTLLASLWWGSLALALGAVVWMFGLVLGRLGRERSGRARDVDRK
                     RLREACLAIVSGAGETAEGLRPFQHRARLMAEGLLEFGVIVRGAERERLIAAYRLMAA
                     DARFRERLFAGSRAGRLVAAEALALFPSDETE"
     gene            complement(402..1472)
                     /locus_tag="VF633_01535"
     CDS             complement(402..1472)
                     /locus_tag="VF633_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEX8469769.1"
                     /translation="MPMHAVPVEPAAIVVQARTQSVYDSAVANRRAGRHPAALAGFQA
                     VIDAEPANLDARLNLGLTLLALDRLDDAEAAFEGVLEVEPDYVDARVGLVQAARRRGD
                     AAAARRQLDLAAAFAPDRKDVQALSAAIDAPLWRFDLDVSRSRLSHDLPDWSSERLGG
                     ARRLDERTAAGLSIERTERFGDEDVYLEGQLDREWGRASLFLAIGGAPGADYRAQRAV
                     RIGGATPLGAGFAATIEAGAARYATGTVRNFQPGLTASMASARLVLAARWILVWDEQD
                     RSRSGYAIRASAAFTDRSRVTLSYADAPETSEGVTVDVRATAIGLEVDLVERITARLT
                     LVQEDRASYRRDEIAVGLGVRF"
     gene            complement(1447..1857)
                     /locus_tag="VF633_01540"
     CDS             complement(1447..1857)
                     /locus_tag="VF633_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8469770.1"
                     /translation="MARILIVDDDELLRALLQYRLSADGHDVLALEDGSRVLATIEES
                     RPDLIVLDAMMPVMDGFEILRRLKTGTSPDSTPVIMLTALKREADVVGALELGAADYL
                     AKPFIPDELSQRIRRVLAAIPSAETSDAHARRPG"
     gene            complement(2530..4614)
                     /locus_tag="VF633_01545"
     CDS             complement(2530..4614)
                     /locus_tag="VF633_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8469771.1"
                     /translation="MLRESEARLRFMQQLEERLFRSVEAGEAMGAAAELLGRHLSASR
                     CAYADVDPDTDRFWIRSDYTAPGVESSAGAYSLDLFGPRASADMREGRTLVVRDVAEE
                     LEPGEGREMFQAIGINAIICCPLVKNGRLAAMMALHQTEARDWTPAEIALVNEVVERC
                     WAHVERVGAEARLRESEERLRLAVESADVGFWDVDTVRERLIWPARTKAMFGISPEVP
                     VTMQDFYDGLHPEDRAATIDAFTAAADPGRRALYDVEYRTVGKEDGVQRWVAAKGRGV
                     FDGAGRCLRVAGTAVEVTARKAAEEALRELNTTLEARVAQAIAEREEAQAALRQSQKM
                     EAMGQLTGGVAHDFNNLLTPIVGALDMLERRKLGGEREQRLISGAMQSAERARTLVQR
                     LLAFARRQPLQPVPVDIPKLVHGMGDLVASTTGPQIRVVVDAPEDLPAATADPNQIEM
                     ALLNLSVNARDAMPEGGTLRISASAETVGLGAQANLSPGSYIRMSVADTGAGMDEATL
                     QRAVEPFFSTKGVGQGTGLGLSMVHGLASQLGGALMIQSTPGLGTNVELWLPQSLDRP
                     SGDPSEPNVAKAGRTSGTALLVDDEVLVRASTADMLMDLGYDVVEASSGEEALRLLEE
                     DRHFDILVTDHLMPGLNGTDLARLVRTSRPSTPVLLVSGYGEREGLDPDLPRLTKPFR
                     KDELAARLADLR"
     gene            complement(4967..5380)
                     /locus_tag="VF633_01550"
     CDS             complement(4967..5380)
                     /locus_tag="VF633_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX8469772.1"
                     /translation="MQLERLVYCSRATVPTDSLLVVAEILAVSQRNNDRDKLTGALAI
                     NDGWFLQVIEGSADNIDRLLGRLKSDPRHRDVEILQRGPVTSRLFDKWSMASARITPS
                     LRSDLVALVNECRTSPADAVTALAQIVAINEAANT"
     gene            complement(5694..7481)
                     /locus_tag="VF633_01555"
     CDS             complement(5694..7481)
                     /locus_tag="VF633_01555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8469773.1"
                     /translation="MRISTLTNWAYAATLILTGLSGAAFISAAHSAQEERLAVEEHLR
                     IDVIAEDLALGVEKLSDEARLYAMRRTARHRDSYVHQREVVGSLDGALERARAADPVA
                     GERAAIDEAERQLVELERIEDAAVAAVAEGRTDLARSMLFGAEHERAQAAVLTALESF
                     RALATARTETRMNAAHRASDLAGLVARITLGITALVFLAVLYFILRRRVALPLARMAG
                     IVTKLADHDYDVDVPDDRRRDEIGEMAQAIQVFRQTGLERERLEAERLADQRVKDGIL
                     QMMHRLQACETRDELAGVVACFAPQTFPALAGALYVMDQGGNGLVRAADWLNPVQADA
                     VFAATACWGLRRGRPHVSNAEAHDIACPHIHSGDAIGLCLPLHAQGETIGLLYLEERP
                     EIEDSASRVFLDLMADNIALALANLRLRERLADLAVLDGLTGLKNRRALDQRLASLSE
                     EEAVACVMIDIDHFKRFNDTFGHDAGDAVMTHVAQMMKETLGAAGEAYRFGGEEFTLV
                     IPGASAADARAHAEALRLRISEAQLAHHGRMLGRISISLGVAAAPGDGPPATLLRQAD
                     GALLHAKQNGRDRTVLAGELSPDVQRQRA"
     gene            complement(7716..8219)
                     /locus_tag="VF633_01560"
     CDS             complement(7716..8219)
                     /locus_tag="VF633_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEX8469774.1"
                     /translation="MTNSISTPARQPALVFLAVGGIAAVTASAAATSSLALGFFPWAM
                     FVGWVAWYTRPTSARQGVATWLCLLGGLAFGALAVMALRTLTPSLGIFALSAVVFVVA
                     LTVVSMRNLRLVDNIPAWFLGLIAFFASHMEPGVISVAELGATSAMGVGGGWLSMQLQ
                     RRLAVAH"
     gene            complement(8280..9200)
                     /locus_tag="VF633_01565"
     CDS             complement(8280..9200)
                     /locus_tag="VF633_01565"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8469775.1"
                     /translation="MSKQVILVTGASSGFGLMTAKALAQAGHTVYATMRETEGKNAPR
                     VIEVAEWSKAEGVDLRTAELDVQSDASADAGVAYILKDAGRLDVIVHNAGHMMFGPAE
                     AFTPEQFIQQYDVNVLGAQRVNRAALPYLRGQGKALMIWVGSSSTRGGTPPFLAPYFA
                     AKAAMDALAVSYSTELALWGIETTIMVPGAFTKGTNHFAHSGSPADTERAAEYEAGPY
                     KGIVDKALKGLAGLEPADADPAEVAREIVRVVDAPFGKRPFRVHVDPSQDGAEIVNGV
                     ADRMRREMYRNIGLQDLLSPAETWKHAADV"
     gene            complement(9301..10041)
                     /locus_tag="VF633_01570"
     CDS             complement(9301..10041)
                     /locus_tag="VF633_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8469776.1"
                     /translation="MTNTTHKVAIVTGAAGGIGAAVSERLAQDGFIVVVNYAGKAEPA
                     EALVARIEAAGGRAVAAQADISDSAAVARLFDTTEAAFGGVDVLVNNAGIMQLATIGE
                     SDDALFDRQIAVNLKGTFNTLREASRRLRDGGRIINFSSSVTTMLQPTYGVYAATKAA
                     VEAMTSILTKELRGRAITVNAIAPGPTATKLFLDGKPQEVIDRLSKMAPLERLGQPED
                     IAAAVAFLAGPDGAWINGQTLRANGGIV"
     gene            10157..11104
                     /locus_tag="VF633_01575"
     CDS             10157..11104
                     /locus_tag="VF633_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8469777.1"
                     /translation="MDRFDAMRVFTRIVERRSFTQAAEDLGLPRSSVTDAVKGLEARL
                     GVRLLQRTTRSVSPTLDGEAYYRRCIDLISDLEDAEAGFTGGKPSGLVRIDVHGTQAR
                     HFLLPGLPKFLETYPDLQLHIGEAHQAMDMVREGYDCMLRAGELTVDSSLIRRRLATL
                     QRGTYASPDYLSRHGVPLTPDDLERKGHRMVGYYAPDAPEIAPFDFAIDGKHRKMVLP
                     AAITVIGPETNVASAGLGLGLIQVPKYRVEVDVAAGVLVEVLKDYPPAVLPVHVLYSH
                     TRQLSPRLRAVIEWMVEQFRERTAEAAARNIKGETQTAR"
     gene            complement(11153..11956)
                     /locus_tag="VF633_01580"
                     /pseudo
     CDS             complement(11153..11956)
                     /locus_tag="VF633_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
     gene            12059..12955
                     /locus_tag="VF633_01585"
     CDS             12059..12955
                     /locus_tag="VF633_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8469778.1"
                     /translation="MRSTEFAELSAFVAVAQEKSFRRAAVKLNLRPSTLSHSLRSLEA
                     RLKVRLLARTTRTVAPTEAGQALLDRIAPAFADITAAVTDIHELSGQPQGRLRINAPF
                     TAATMVMAPILGLFARQWPDVTLEIVINEKFVDIVQEGFDAGIRIGDDVAQDMVGVRL
                     TQDFRTAVVASPAYFETRPQPETPYDLHDHLCVAQRQPGSGGLFRWEFVNDGRALTVA
                     VDGPLILDSHDLMISAALQGVGLACVLEHVVADHIASGRLVQVLRPWCETYPGFYLYY
                     PRHRQGSASLRALVDILRRQAG"
     gene            13461..14003
                     /locus_tag="VF633_01590"
     CDS             13461..14003
                     /locus_tag="VF633_01590"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HEX8469779.1"
                     /translation="MPEPKPRRRFPHKLYLVLLDNRDGPAIRDGRSLWALQRPMSYYS
                     GPGRDEEIIVPAGFVTDLTSIPRLVWSFYPPDGPWVKAAIVHDFLYDTQGDGRWNHTV
                     GVTRETPYSRKESDDILLEGMIERNVGRWERLVIWIAVNFGGQRGWDRAAEIRAGRQK
                     LGKPIANKGEPAVPAPKASH"
BASE COUNT         2526 a         4580 c         4493 g         2447 t
ORIGIN      
        1 ctcggtctcg tctgacggaa acaaggcgag ggcttcggcc gcaaccagac gccctgctct
       61 gcttccagcg aacagccgct cgcggaaacg cgcatccgcc gccatcagcc ggtaggctgc
      121 aatcaaacgc tcccgctcag cgccccgaac gatcactcca aactccagaa ggccctccgc
      181 catcaaccgg gcccgatgct gaaacggtcg caaaccttcg gcggtctcgc cggctccgct
      241 cacgatcgcc agacaggcct cccgcaggcg cttgcggtcc acatcgcgcg cccggccgct
      301 tcgctcgcga cccagccggc ccagcaccag gccgaacatc cagaccacgg cgccgagagc
      361 aagggcgaga gatccccacc acagggaggc aagaagagtc atcagaaccg gacgccgagt
      421 ccgaccgcga tctcgtcccg tcggtagctg gcgcggtcct cctgaaccag ggtcagtcgg
      481 gcggtgatgc gttcgaccag atcaacttcg aggccaatgg cagtcgcccg cacgtccacc
      541 gtcaccccct cggacgtttc cggcgcgtca gcatagctga gggtcacacg ggatcggtcc
      601 gtgaaggctg cgctggcgcg tatcgcatag ccggatcgac tgcggtcctg ctcgtcccag
      661 acgagtatcc aacgggccgc cagcaccagt ctggctgagg ccatgctcgc cgtcagtccc
      721 ggctggaagt tgcgaacagt gcccgtggcg tagcgcgccg cgccggcttc gatcgtagct
      781 gcgaagccgg cgccgagggg ggtggccccg ccgatccgca cggcccgctg ggcccggtag
      841 tctgctccag gcgccccgcc gatggccaaa aaaagagagg cgcgccccca ctcccgatcg
      901 agctggcctt ccagatagac gtcctcatcg ccgaaccgtt cggtccgctc gatggagagt
      961 cccgctgcag tgcgttcgtc gaggcgtctc gctccgccaa gacgctcgct gctccagtcg
     1021 gggaggtcat gcgacagccg actgcgggaa acatcgaggt caaatctcca tagcggcgcg
     1081 tctatggcgg ccgagagcgc ctgaacgtct ttacggtccg gcgcgaaggc cgcggccaga
     1141 tcgagctggc gtcgggccgc agcggcgtcg ccgcgccgac gcgccgcttg gaccagtcca
     1201 acgcgggcgt cgacgtagtc aggctcgacc tccagaaccc cttcaaacgc cgcttcggca
     1261 tcatccagtc tgtccagcgc cagcagagtc agtccaaggt tcagcctggc gtccagattg
     1321 gccggctcgg cgtcgatcac ggcctgaaag ccagccaagg cagccgggtg gcgacctgct
     1381 cggcgatttg cgacggcgct atcgtagacg ctttgggttc gagcctgaac gacgatggcc
     1441 gcaggttcaa ccgggacggc gtgcatgggc atctgaggtc tcggcgctgg gaatggcagc
     1501 cagaacgcgt cgtatccgct ggctaagttc gtcgggaatg aagggcttgg ccagataatc
     1561 ggccgcgccg agctcaaggg ctccgacaac atcggcctcc cgcttgagcg ctgtcagcat
     1621 gatcacaggc gtggagtccg gactcgtccc ggtcttcagg cggcgcagga tctcgaagcc
     1681 gtccataacc ggcatcatcg catcaaggac gatcaggtcc gggcggcttt cttcaatagt
     1741 ggcgagcacc cgcgacccat cttccagggc gagcacgtca tgaccatcgg ctgagagacg
     1801 atattgaagc agagccctga gcagctcgtc atcgtcgaca atcagaatac gcgccattgg
     1861 ctgacccagt cacaatctcg gccacaccaa gagcgtcaat catgccagct tgaggtaaac
     1921 caatcgctat tgtgagcgat ttggccgtag cgtccggtcc actgcttaac ccgagcttgg
     1981 ggggcgccag ccggactggg gattgggttg atcaaagcgc cacatacctg gataggcggc
     2041 cggcgttcca gctcaggtgc tctaggacta ttcctctgcc gtcttgaagg tccacgcgct
     2101 ctgctccaac actaggcgaa tctccccgcg cctgcgcgtc atcgaatgat ggcggagcat
     2161 ctccgcgagc taggatggag tggcggccac gaccagcgag ccggaaacca gagccgcaga
     2221 ttaagacgac gagtatcaag cattaggtcc caccgggctg tgagaggttc tgaaccgtcg
     2281 gccaggccgg aggagagtgc agtattcgac catggcgggt cccgggccgg ctttctcggc
     2341 gcatctatgt catcgagaga tgccgatcgc ttcgctgaga tgcaacggaa cctcaagccg
     2401 ggacggtcga gaaccacatc gcgcgcgtcc caaagcggac cttcccgatg actgggaaga
     2461 gtcagataga gaacttgcca agaacgaggt acattgacct agagccccgt aagtttgcca
     2521 aatgtggcgt cagcgcaggt cggcaagacg tgcggcgagc tcatccttgc gaaagggttt
     2581 ggtcaagcgc ggcaaatccg gatccaaccc ttcgcgctct ccgtatcctg acacgagaag
     2641 cacgggcgtg cttggcctgg acgtgcgtac caaccgcgcc aagtcggtcc cgttgagccc
     2701 cggcatcagg tgatcggtga cgaggatatc gaaatggcgg tcctcttcca aaagacgaag
     2761 cgcctcctcc ccggacgagg cctccacgac atcatagccg aggtccatca gcatgtccgc
     2821 ggtgctggct ctcacaagaa cctcgtcatc caccagaagc gccgtcccgc tcgtgcgacc
     2881 cgccttggcc acgttcggct cggacgggtc gccagaaggg cgatccaggc tctggggaag
     2941 ccacagttcg acgttcgttc ctagtccggg ggtgctctgt atcatcagag ctcctccgag
     3001 ctgcgacgcc agcccatgaa ccatcgacag cccgagcccc gtgccttgcc caacaccttt
     3061 ggtcgagaag aagggctcga ccgcgcgttg aagggtggct tcgtccatac cggcgccagt
     3121 gtcagcaacc gacatccgga tatacgaacc cgggctaagg tttgcttggg caccaagacc
     3181 gaccgtctca gcgctcgccg agattctcaa agtcccaccc tccggcatgg cgtcgcgcgc
     3241 gttcacgctc aaattcagaa gcgccatctc gatctggttc ggatccgctg tcgctgccgg
     3301 aagatcttcc ggtgcatcaa cgaccaccct gatctgggga cccgtcgtgc tggcgacgag
     3361 gtcccccatg ccgtgaacga gcttggggat atcgaccggc acgggctgaa gcggttggcg
     3421 ccgggcgaac gccagcagcc tttgcacgag cgtcctggct ctttcggcgg actgcatcgc
     3481 tcccgagatc aggcgttgct cacgttcgcc tccaagcttt ctccgctcga gcatgtccaa
     3541 cgccccgaca atcggtgtca aaaggttgtt gaagtcatgc gccacaccgc cggtgagttg
     3601 gcccatcgcc tccatctttt ggctctgcct caacgccgcc tgcgcctcct cgcgctctgc
     3661 gatcgcttga gcgacacggg cctcaagcgt cgtgttcaac tcccggaggg cttcctcggc
     3721 ggcctttcgc gccgtaacct caacggctgt gccggcgacc ctcaggcacc ggcctgcccc
     3781 gtcaaacacg cccctcccct tggccgcgac ccagcgctgt acgccgtctt ccttgccgac
     3841 agttcgatat tcgacgtcat agagcgcgcg ccggcctgga tccgccgcag ccgtgaaggc
     3901 gtcgatcgtc gccgcccgat cttccggatg aagtccgtcg tagaagtctt gcatcgtcac
     3961 cggcacttcc ggcgagatcc cgaacatggc tttggttcgc gctggccaga tgaggcgttc
     4021 cctgaccgtg tccacatccc agaacccgac gtccgcgctc tccacggcca gacgaagtct
     4081 ttcctcgctt tcccgcaacc gagcctcggc cccgacacgc tccacatgcg cccagcatcg
     4141 ctcgaccact tcattgacca gggcgatttc ggccggagtc cagtcacggg cttctgtctg
     4201 gtgaagcgcc atcatggcgg ccagtcgtcc gttcttcacc agcgggcagc agatgatggc
     4261 gttgatcccg atggcctgga acatctcgcg gccctcgccc ggctcgagtt cttcggccac
     4321 gtcgcgcacg acaagggtcc gtccctcacg catatccgcc gacgctcgcg gtccgaacag
     4381 gtcaagactg tacgccccgg ctgagctctc cacccctggc gcggtatagt cgctgcggat
     4441 ccagaaccgg tcggtatcag gatcgacgtc agcgtaggcg cagcgggacg ccgagagatg
     4501 gcggcccagc aattcggcgg ccgcgcccat ggcttcgccg gcctcgacgg atcgaaacaa
     4561 ccgctcctca agttgctgca tgaagcggag ccgcgcctcg ctctcgcgca acatggcctt
     4621 ggactggaga cgctcagtca cggtcctcgc cgtcctgaag atgcgactgg cggtctcgag
     4681 atcatcgggt gacatccccc gcgcatcggg tcgcctcaat aacacccgga gacgtctcgc
     4741 gtagtcgagc acaggggtgg gcgccgctgc agccgccgtc gccgctcatg gagccaggat
     4801 ttcgaagagc ctcgtgatca atcaaacagg cttcctcttc gtgtccgcgc atccgcgcgc
     4861 cgcggtcctt agagctatgc tttagttcag aatcgcggag gcgcaatcat cgccatagcc
     4921 tttagttggc ggacgcttgc ccgctcgctt gctgaaaccg gccgactcag gtgttggcgg
     4981 cctcgttgat ggcgacgatt tgtgcaagcg ccgtcaccgc atccgccggc gaagtacggc
     5041 actcgttgac cagcgcgacc aggtcggaac gaaggctcgg cgtgatccgt gcgctagcca
     5101 tagaccactt gtcgaaaagt cgactggtga ccggaccgcg ttgcaggatc tcgacgtccc
     5161 tgtgacgagg gtctgacttc agccgaccta acaatcggtc gatgttgtct gctgaccctt
     5221 caataacttg tagaaaccaa ccatcgttga tggccaaggc gccggttagt ttgtcgcgat
     5281 cattgttccg ctgcgaaacc gccaggattt cagccacgac taagagggag tcggtcggca
     5341 cggtggctcg gctacagtag acgagacgtt caagttgcat gttccggacc tccaacctaa
     5401 gaccgcaaac accgccaagc ggactcggtt gccggaagcc gaccgggacg gggcttgcca
     5461 gcgttggcaa tcctaccatc atcgcaagtc gtatctccgc ctgttctgga cggtcaacgt
     5521 ccggctttcg ttgtctcaaa tgtggtcgcc accgtggtcg cggggcggac atggttcaca
     5581 gaagcccctt ctggagaggc atcgcctgcg gttctcaagg gtgtctgagt gctgacgcct
     5641 aaaagcagac gtctaacggc gcgaagcccc ctgtgtactg cgccgcggtc ggatcaggct
     5701 cgctgacgct gaacgtccgg gctcaactcg cccgcaagta ctgtcctgtc acgaccgttt
     5761 tgtttcgcgt gcagcaaggc gccgtccgcc tgtcggagaa gcgtcgcggg cggaccatca
     5821 ccaggagccg ccgccacccc cagagagatg gagatgcgtc cgagcatccg gccatggtgg
     5881 gcgagctggg cttcactgat ccggagcctg agcgcctccg catgcgcgcg agcgtcagcg
     5941 gcgcttgcgc cggggatgac gagggtgaac tcctcgccgc cgaagcgata agcctccccc
     6001 gccgcgccga gtgtttcttt catcatctgc gccacatggg tcatcacggc gtctccagcg
     6061 tcatgaccga aagtgtcgtt gaagcgcttg aaatggtcga tgtcgatcat gacacaggcg
     6121 accgcctctt cctcagaaag gctggcgagc cgctgatcca gcgcccggcg gttcttcagt
     6181 cccgtgaggc cgtccagcac ggccaggtcg gccagccgct cgcgcaggcg caaattcgcc
     6241 aaggccaggg cgatgttgtc ggccatcagg tcgagaaaca cccgggaggc gctgtcctca
     6301 atctccggcc tctcctcgag atagagaagt ccgatcgtct cgccctgcgc atgaagcgga
     6361 aggcagaggc cgatggcgtc cccggagtgg atgtgagggc aggcgatatc gtgcgcctcg
     6421 gcattgctga cgtgaggccg accgcgacgc aggccccagc aggcggtcgc cgcgaacact
     6481 gcatccgcct gaacgggatt gagccagtcc gctgcgcgaa ccaggccgtt gccgccctgg
     6541 tccatcacat agagcgcgcc agccagtgcc ggaaaggtct gcggcgcgaa acaggccacc
     6601 acaccggcca actcgtcgcg ggtctcacaa gcctggagac gatgcatcat ctgcaggatg
     6661 ccgtccttga cccgctgatc agcgagacgc tcggcctcca gccgctcacg ctccagaccg
     6721 gtctgacgaa acacctggat cgcctgggcc atctctccga tttcgtcacg ccttcggtcg
     6781 tcgggcacat cgacatcgta gtcgtggtcg gcgagcttcg tgacgatacc ggccatccgg
     6841 gccagcggca gggccacgcg gcgtcgcagg atgaagtaga gcactgccag aaacacgaga
     6901 gccgtgatcc cgagcgtgat ccgggccacc aaccccgcca gatcgctcgc cctatgcgct
     6961 gcgttcatgc gggtctccgt ccgggcggtc gccaaggctc ggaaactctc gagagcagtg
     7021 aggacggcgg cctgggcgcg ctcgtgttcg gccccgaaca acatgctgcg ggcaaggtcc
     7081 gtgcggccct ccgcaacggc ggcaaccgcc gcgtcctcga tcctctcgag ctctacgagc
     7141 tgacgctccg cttcatcgat cgcagcccgc tcccccgcaa caggatctgc ggcgcgcgcc
     7201 cgttccagcg ccccgtcgag cgagccaacg acctcgcgct ggtggacata actgtcgcgg
     7261 tgccgggccg tcctgcgcat ggcgtaaagt cgcgcctcgt cagacagttt ttccacgccc
     7321 agagccaagt cctcggcgat cacatcgata cgcagatgct cttcgacagc caggcgctct
     7381 tcctgagccg aatgagccgc gctgatgaag gcggcgcctg agagacctgt gaggatcagc
     7441 gttgctgcat aggcccagtt ggtgagtgta ctgatacgca tttgagcaga ttacatgaac
     7501 agcatttccc gccgattaat ccctgcctac gtaaaacctg agattatcga aaagagcgaa
     7561 atctacctga tgcggcggct gcaaatggag ggtgaccgtg cccattcgag aaggtgggcg
     7621 tagcgcgagt atcgccggac atttgaacct gcaacccgag cgtggacgcc cgcaagggcg
     7681 acggaggaga aacctggccg tcgtccctac ggggctcaat gtgcgactgc aagacgccgc
     7741 tgcaattgca ttgacagcca gccccctccc acgcccatgg ccgacgttgc tcccaattcg
     7801 gccaccgaaa tcacaccggg ctccatgtgt gaggcgaaga aggcgatcag cccaaggaac
     7861 caggcgggaa tgttatcgac caagcggagg tttcgcatgg aaaccactgt gagggcgacg
     7921 acgaagacga ccgcgcttag tgcgaagatg ccgaggcttg gcgtcagcgt gcggagcgcc
     7981 atgacagcga gcgccccgaa cgcgagaccg cccagcagac acagccaggt cgctacaccc
     8041 tggcgagcag aagtggggcg tgtgtaccag gcgacccagc ccacaaacat ggcccagggg
     8101 aagaagccga gggcaaggct ggaggtggct gcggctgagg cggtgacggc ggcgatgccg
     8161 ccgacggcga ggaagacaag ggccggctgg cgggcgggtg tggaaatgga attagtcatt
     8221 gaagggctcc gatcaaaaag gcgtccgccg acgcgggagc gccggcggaa aggttgaggt
     8281 cagacgtcgg cggcgtgctt ccaggtctcg gccgggctga gcaggtcctg caggccgatg
     8341 ttgcggtaca tctcgcggcg catccggtcg gcgacgccgt tgacgatctc ggcgccgtcc
     8401 tgcgacggat cgacgtggac ccggaacggg cgcttgccga acggggcgtc gacgacgcgc
     8461 acgatctccc gggccacctc ggccggatcg gcgtctgcag gttccagacc ggccagaccc
     8521 ttcagcgcct tgtcgacgat gcccttatag ggaccggctt cgtattcggc ggcgcgctcg
     8581 gtgtccgcgg gagaaccgga gtgggcgaag tggttggtgc ccttggtgaa ggcgccgggg
     8641 accatgattg tcgtctcgat cccccacagg gccagttcgg tcgaatagga gaccgccagc
     8701 gcgtccatcg ccgccttggc ggcgaagtat ggcgccagga acggcggggt gccgccgcgt
     8761 gtcgaggacg atccgaccca gatcatcagg gccttgccct gcccgcgcag gtaggggagc
     8821 gcggcgcggt tgacccgttg ggcgccgaga acgttgacgt cgtactgctg gatgaactgc
     8881 tccggcgtga aggcttcggc cggaccgaac atcatgtgcc cggcgttgtg gacgatgacg
     8941 tcgaggcggc cggcgtcctt gaggatgtag gcgacgccgg cgtcagccga ggcgtccgac
     9001 tggacgtcga gttcggcggt gcgcaggtcg acgccttcgg ccttggacca ttcagcgacc
     9061 tcgatgacgc ggggggcgtt cttgccctcg gtctcgcgca tggtggcgta gacggtgtga
     9121 ccggcctggg cgagggcctt ggcggtcatg aggccgaagc cggaggaggc gccggtgacg
     9181 aggatgactt gcttggacat tgtggtgttc cttgttcggg gagccctccc gcaccgccct
     9241 cgggcgatgc ggctcaggcc gtgatgtgga tgaggttcaa tcgggtcggt gaggcgaggc
     9301 tcagacgatg ccgccgttgg cgcgcagggt ctggccgttg atccaggcgc cgtccggtcc
     9361 ggccaggaag gccacggcgg cggcgatgtc ctcgggctga ccgaggcgtt ccagcggcgc
     9421 catcttcgac agacgatcga tgacctcctg cggcttgccg tcgaggaaca gcttggtggc
     9481 cgtcggtcca ggggcgatgg cgttgacggt gatggcccgg ccgcgcagtt ccttggtcag
     9541 gatgctggtc atggcctcga ccgccgcctt tgtcgctgcg tagacgccgt aggtcggctg
     9601 cagcatcgtc gtgacgctgg acgagaagtt gatgatccgg ccgccgtcgc gcaggcggcg
     9661 cgaggcttcg cgcagggtgt tgaacgtgcc cttcaggttc accgcgatct ggcggtcgaa
     9721 cagggcgtcg tcgctctcgc cgatcgtggc gagttgcatg atgccggcgt tgttgacgag
     9781 gacatccacg ccaccgaacg cggcttccgt ggtgtcgaac aggcgggcca ccgcggccga
     9841 gtcagagatg tcggcctggg cggcgacggc gcgaccaccg gcggcttcga tcctggcgac
     9901 caaggcctcg gcgggttcgg ccttaccggc gtagttgacg acgacgatga agccgtcttg
     9961 cgccagacgc tccgagacgg cggcgccgat gccgccggct gcgcccgtga cgatggcgac
    10021 tttgtgagtg gtgttggtca tggtcttttt ccttgctgtg gccaccgcgt gtcggcgtcc
    10081 gatgaccaga aggtgcctgt atccagcggc gcgataatca ggcacaaacc ggcatcacaa
    10141 tccgaaagtg cgaacaatgg atcggttcga tgcgatgcgg gtcttcaccc ggatcgtgga
    10201 gcgacgcagc ttcacccagg ccgctgagga tctcggtcta ccgcgctcat ccgtgaccga
    10261 tgctgtgaaa ggactggagg cgcggcttgg cgtccgcctc ctgcagcgca ccacgcgaag
    10321 cgtcagcccg acactggacg gggaagccta ttaccgccgc tgtattgatc tgatctccga
    10381 cctcgaagat gcggaggccg ggttcactgg aggcaaaccg tcaggtctgg tgcggattga
    10441 tgtccacggc acccaggccc gccacttcct tcttcccggc ttgccgaagt tccttgaaac
    10501 ctaccccgat ctgcagcttc acatcggtga ggcgcaccag gccatggaca tggttcgcga
    10561 aggctacgac tgcatgctga gagccgggga actaacagtg gacagctccc tgatccgtcg
    10621 ccgtcttgcg accctgcagc gagggacata cgcaagcccc gactaccttt cgcgccacgg
    10681 ggttcccctg acgcccgatg atctcgagcg caagggtcat cggatggtgg gctactacgc
    10741 gccggacgct ccggagatcg cgcccttcga cttcgctatc gacggcaagc atcgcaaaat
    10801 ggtgctcccc gccgcgatca ccgtcatcgg cccggaaacg aacgtcgcat cggcgggcct
    10861 cgggcttggc ttaatccagg tgcccaaata ccgcgtcgag gtggatgtgg cggcgggcgt
    10921 cctggttgag gtcctcaagg actatccgcc cgccgtcctg ccggtccacg tcctgtactc
    10981 tcacacgcgc cagctctctc ctcgcctgcg cgcggtcatc gagtggatgg tggagcagtt
    11041 ccgggagcga acagcggaag ctgcggccag aaacatcaag ggggaaacgc agaccgcgag
    11101 atgaggcccg ctgccgtcat cacaggtggc gggccctgtc acgcgtttcg gtttagcgga
    11161 caccgggcag attaagaccg ccgtcaacca agatcgtctg gccggtgaca aaccgcgcgc
    11221 cgtcggaggc cagccaaacc gcgacatcgg cgacgtctgc gggctggcca agacgcccga
    11281 gcggggtctg gcgggtcacg gccgccgcgg cggcggcccc gtcgaggcgg gcgaacatcg
    11341 acgtatcaat cgccccgggc aggacgttgt tgatcctgat cccgtgaggg ccgccctcga
    11401 ctgcgagacc ggccaccatc gcatccagac cgcccttgct ggcggcatag gcggaggcgc
    11461 cggcgagagc gttcttggcg acgaaggacg aggtgttgac gattgccccc ccctgcccgc
    11521 ccgcgatcat ggcgatcatc tggtgcttca tggaaagtca ggcgcctttg aggttcaccc
    11581 ggatgacctg atcgaagtcg gcttccgtca gcgccgtgat ggggccgaac acgccctcga
    11641 ttccggcatt gttgaacgtg gcgtccagac ggccgaagcg cgcgatcgtc ttggcgacca
    11701 gcgacgcgac ctcggcttcg accgagacat cggtcgcgac gacgaaggcg accccggcct
    11761 ggtcaacgat cagagcggcc gtctcgtcga gttcggcccg gcggcggccg gcgagcacga
    11821 cgctcgctcc ctccatagcg aagcgcagag cggccgcgcg gccgatgccg ctgccggccc
    11881 cggtgacgat ggcgacccgc ccggccattc gggcttgtcc gtcggtggcg tccgtcgtgg
    11941 aagcgatgtg atccatggtg aaaatcctcg tgagttacgg aggtctatct aaggccggcc
    12001 cacccatggc gataatccat gctatatggc atcagctgat gaacacaggc catcaatcat
    12061 gcggtccact gagtttgcgg aattgtcggc cttcgttgct gttgcgcagg aaaagagctt
    12121 tcgacgcgcg gcagtgaagc taaacctcag gccgtcaacg ctcagccact ccctgcgctc
    12181 tctggaggcg cgtttgaagg ttcggctttt ggcgcgcacg acacgtacgg tcgcgccgac
    12241 ggaggccggc caggctttgc tggacaggat cgctcccgct ttcgccgaca tcaccgctgc
    12301 cgtgacggat atccatgagc tgagcgggca gccgcaggga cgcctgcgga tcaacgcgcc
    12361 cttcacggcc gcgaccatgg tgatggcgcc gatccttgga ctgttcgcac ggcaatggcc
    12421 ggacgtgacg ctggaaatcg tcatcaacga gaagttcgtc gacatcgttc aggagggttt
    12481 cgacgccggt atccggatcg gcgatgatgt ggcgcaggat atggtgggcg tccgtctaac
    12541 gcaggacttc agaacggctg tcgtcgcctc gccggcctat ttcgagaccc gacctcagcc
    12601 ggagaccccc tacgatctgc atgaccatct gtgcgtggcg cagcgtcagc cgggcagtgg
    12661 cggcctgttc cgatgggagt tcgtcaatga cggtagagcg ctcactgttg cggtcgatgg
    12721 accgctaatc ctcgacagcc acgacctgat gatttcggcg gcgctccagg gcgttgggct
    12781 ggcctgcgtt ctggagcatg tcgtggccga ccacatcgcc agcgggcggc tcgtgcaggt
    12841 cctgcgtccc tggtgcgaaa cctatccagg cttctacctc tactatcccc gacatcgcca
    12901 aggctcggcc agcctgcgtg ccttggtcga cattctgcgc cgtcaggccg gctgaacccc
    12961 gctcgttagt ggccgaaggc cggccggttt ctcgtcctcc gcaacggcac ttgggcttac
    13021 gatcccttcg tataatgacc gtctggatca tgaccgcagg tataatcgac gggctccgcg
    13081 ccgccgagca agagtggctg tgtcgaaggg gatggccgaa gaaggcgatc tgggggtcgt
    13141 cgttgacgca aagggcgaag ccgccggcca ccgcctgcgc cttgatcttg tagtgtttgg
    13201 tcaggaccat ggccccgaag caggcgaaga agaggcaggc cttctcggac cgggcgacgt
    13261 cgtgaagaac tccgtgcgcc acctgataaa ttcgattgta ctcgctcaac ggcatcagaa
    13321 accggctcaa tggcgtcact cccttgaagg tctgcgcacc gtagccgatc accggtcccg
    13381 atcgcgaacg cgatcagcct gtgcgaccga cgccggatcc aggggccctg cggctccgga
    13441 ccgtttcatt ggatcgtgtt atgcctgaac ccaaaccgcg ccgccgtttt ccgcacaaac
    13501 tctatctggt gttgctcgac aaccgggacg gaccggcgat ccgcgacggc cgctcgctgt
    13561 gggccctgca gcggccgatg tcctactaca gcggtcccgg acgcgacgag gagatcatcg
    13621 tgccggccgg gttcgtcaca gacctgacct cgatcccgag actggtctgg tccttctatc
    13681 cgcccgacgg tccctgggtg aaggcggcga tcgtccacga cttcctctac gacacccagg
    13741 gcgatggtcg gtggaaccac accgtcgggg tgacccggga gacgccctac agccgcaagg
    13801 aatccgacga catccttctg gaagggatga tcgaacggaa cgtcggccgc tgggaacggc
    13861 tggtgatctg gatcgccgta aatttcggtg gccaaagggg gtgggaccgt gccgccgaaa
    13921 tccgcgccgg acgccagaag ctcggcaagc ctatcgcgaa caagggcgag ccggccgtgc
    13981 ccgcccccaa ggcctcacac tgaaggcctc atcatccgcg cctcgctcga cctacttcag
    14041 ctgaca
//
LOCUS       DASVTM010000062         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_751291,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000062 DASVTM010000000
VERSION     DASVTM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751291"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="VF633_01595"
     CDS             <1..109
                     /locus_tag="VF633_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX8469780.1"
                     /translation="KGFLMVSSSPLTRSSHHAGDDFARLKAARLAHTGR"
     gene            117..578
                     /locus_tag="VF633_01600"
     CDS             117..578
                     /locus_tag="VF633_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX8469781.1"
                     /translation="MAVHTVTRILPYEPQQLAALVADVRAYPEFVKWVSSMRVWNARE
                     EAPGVTLVDAEAQVGFSFLKERFSTWVRHDRNLPRVEVGLIRGPFKHLKNRWDFHEDP
                     AGTRLEFMIDFAFRSPILNAMLQANFQLAVGKLISSFEAEAQRRYGAKTEA"
     gene            575..1690
                     /locus_tag="VF633_01605"
     CDS             575..1690
                     /locus_tag="VF633_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8469782.1"
                     /translation="MTEFDFDATVVGAGAVGLACGRALAKRGLTVLVLEKEGHIGQGV
                     SSRNSEVIQGGLYYPTGSLKARFCVEGRRALYGYLESHKIDYRKCGKLVVATQEDEVG
                     HIEQIFEQATTNGIEGLEHLTGAQTRAMEPALNAHAAILSPESGLFASHDYMLALQGE
                     IEDAGGSVVLSTPFERAEPLTGGGFTITAGGEGGATLTTRLLITAPGLQAQHVAALID
                     GFPADHIPAGHFGKGVYFRLTGKAPFDKLIYPPPIAGALGTHYRKDLGGQAVFGPDLV
                     YVETEDYSVDPAKAEEFATYIRRFWPGLPDGALTPDYAGIRPKLHGPGEVQPDFQLRG
                     AEDHGIEGLMALFGIESPGLTSSLAIGEAVADRLLAV"
     gene            complement(1687..2025)
                     /locus_tag="VF633_01610"
     CDS             complement(1687..2025)
                     /locus_tag="VF633_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469783.1"
                     /translation="MDQMTQAKPRNRSLKVWLIVDTGVSILAIVPVAMFGMMSVMASD
                     AGVNAAIMTFIWTGLTFPVAVALSPILAWIAFALRRERTAVILSLLPVLWVIAIIAMF
                     VVGFGDPQPV"
     gene            2088..2666
                     /locus_tag="VF633_01615"
     CDS             2088..2666
                     /locus_tag="VF633_01615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEX8469784.1"
                     /translation="MTEPLLTEREKMLAGSVYDPGDAELAEGRARAVGLTVRINTEPD
                     RVARRALVNELVKAADGAAVIMPGFFCDYGDNIHVGERAFVNANCVFLDCAEIIIGDD
                     FQCGPGVQLLTPEHPLDAAERKAGLESARPIRIGNDVWLGGGAIVLAGVTIGDRAIIG
                     AGAVVTKDVPADVVVAGNPARIIKTLVSPTVD"
     gene            2818..3000
                     /gene="rpmF"
                     /locus_tag="VF633_01620"
     CDS             2818..3000
                     /gene="rpmF"
                     /locus_tag="VF633_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX8469785.1"
                     /translation="MAVPKRKVSPSRRNMRRSHDALGVNAYTEDKDTGELRRSHHIDL
                     KTGTYRGRQVLTPKED"
     gene            3116..>3518
                     /locus_tag="VF633_01625"
     CDS             3116..>3518
                     /locus_tag="VF633_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469786.1"
                     /translation="MIRRATVLTSFAVLALAACGQPKPAEQAEAPAAPTTASAESPPA
                     DPNLVTAEGIGPIRIGQTIGEVQALSGTSDTAIASPDGCNIFHPSRAPAGVFVMTEMG
                     RVSRVTLRDGSTAKTDKGFGVGADASAIKASY"
BASE COUNT          620 a         1192 c         1101 g          605 t
ORIGIN      
        1 caaggggttt ctgatggtct catcctcgcc cctgacccgc agctcgcacc acgccggtga
       61 cgacttcgcc cggctgaagg ccgcgcgtct tgcccatacg ggacgctgac gaccgcttgg
      121 ccgtccacac cgtcacccgc atcctgccct acgaacccca gcagctcgct gcgctggtgg
      181 cggacgtgcg cgcctatccg gagttcgtga agtgggtcag ctccatgcgg gtctggaacg
      241 cgcgtgaaga ggcgccgggc gtcactctcg tcgatgccga ggcgcaggtc ggcttctcgt
      301 tcctcaagga gcggttctcg acctgggtgc ggcatgatcg caacctgccg cgcgtcgagg
      361 tcggcctgat ccggggaccg ttcaaacacc tgaagaaccg ctgggacttc catgaggacc
      421 ccgcaggcac gcggctggag ttcatgatcg acttcgcctt ccggtcgccg atcctgaacg
      481 ccatgctgca ggccaatttt cagctcgcag tcggcaagct gatatccagt ttcgaggccg
      541 aggcgcaacg aaggtatgga gccaaaaccg aagcatgacc gagttcgact tcgacgcgac
      601 cgtggtggga gcaggggccg tgggcctcgc ctgcggacgg gcgttggcga agcgcggact
      661 gaccgttctt gtgctggaga aggaaggcca tatcgggcag ggcgtctcct cgcggaactc
      721 cgaagtcatc caaggcgggc tctactatcc gaccggttcg ctgaaggccc ggttctgcgt
      781 cgagggccgt cgtgcgctgt acggctacct cgagagccac aagatcgact accgcaaatg
      841 cggcaagctg gtcgtggcga cgcaggaaga cgaagtcggc catatcgagc agatattcga
      901 acaggcgacg accaacggca tcgaggggct ggagcatctg accggcgccc agacgcgggc
      961 gatggagccg gcgttgaacg cccacgccgc catcctgtcg cccgagagcg gcctgttcgc
     1021 cagccacgac tatatgctgg ccctgcaagg agagatcgag gacgcgggcg gctcggtcgt
     1081 cctgtccacc ccgttcgagc gcgccgagcc cctgactggc ggcggcttca ccatcacggc
     1141 cggcggggag gggggcgcga ccctgacgac ccgcctgctg atcaccgcgc ccggccttca
     1201 ggcccagcat gtcgctgccc tgatcgacgg cttccccgcc gaccacattc cggcaggcca
     1261 tttcggcaag ggcgtctatt tccgcctgac cggcaaggcc ccgttcgaca agctgatcta
     1321 tccgccgccc atcgccggcg cgctcggcac ccactaccgc aaggacctcg gcgggcaggc
     1381 cgtgttcggt cccgacctgg tctatgtcga gaccgaggac tattcggtcg acccagccaa
     1441 ggccgaagag ttcgcgacct atatccggcg tttctggccg ggccttcccg acggcgccct
     1501 gacgcccgac tacgccggta tccgtcccaa actgcacggc cccggcgagg tccagcccga
     1561 cttccagctt cgcggcgccg aggatcacgg catcgaaggc ttgatggcct tgttcggcat
     1621 cgaaagccct ggcctgacga gttctctggc tatcggtgaa gcggtcgcgg accggctgct
     1681 ggccgtctaa actggctgcg ggtcgccgaa cccgacgacg aacatcgcga tgatggcgat
     1741 cacccaaagc actggaagca ggctcagaat caccgccgtt cgctcccggc gcaaggcaaa
     1801 ggcgatccag gccaatatcg gcgacaaggc caccgcgacc ggaaaggtca ggccggtcca
     1861 gatgaaggtc atgatcgcag cgttgacccc tgcatccgac gccatcaccg acatcattcc
     1921 aaacatcgcc accggcacga ttgcgaggat cgacactccg gtgtccacga tcagccacac
     1981 cttgagactg cggttgcgcg gctttgcctg cgtcatctga tccacgatcg gctccctttg
     2041 ttgatgtcat ttgacgctgc ccagatcatt gcggcaagtc cggcgccatg acggaaccgc
     2101 tcctgaccga acgcgagaag atgctggccg gtagtgtcta tgacccaggc gacgcggaac
     2161 tggccgaagg gcgcgctcgt gccgtgggcc ttaccgtgcg gatcaacacc gagcccgacc
     2221 gggtcgcgcg acgggctctg gtcaacgaac tggtaaaggc ggctgacggc gctgccgtca
     2281 tcatgcccgg ctttttctgc gactacggcg acaacatcca cgtcggcgag cgcgcattcg
     2341 tcaacgccaa ctgcgtcttc ctggattgcg ccgaaatcat catcggcgac gactttcagt
     2401 gtggtccggg cgtgcaactg ctgacgccgg agcatccgct ggacgccgcc gagcgcaagg
     2461 cgggtctcga gagcgcccga ccgatccgaa tcggcaacga cgtctggctg ggcggcggtg
     2521 cgatcgtgct ggctggcgtc accatcggtg atcgggctat catcggcgca ggagccgtcg
     2581 tgacaaagga cgtgccagcg gatgtagtcg tggcggggaa tcctgcacgg atcatcaaga
     2641 cactggtgtc gccgaccgtc gattgaggct ttagccggcg gtgtttcatg accccttgaa
     2701 agttccaacc ggttccggca taagcccccg ctcgcaggcg gctcaccgat gggccgccgc
     2761 ttctgttttt cgcgcttctt cgtgtaggta gcgccgagcc ctgagattga gacggacatg
     2821 gcagttccga aacgcaaagt atcaccctcg cgtcgcaaca tgcgtcgttc gcatgacgcc
     2881 ctcggcgtca acgcctacac cgaggacaag gacaccggcg agctgcgccg ctcgcaccac
     2941 atcgacctga agaccggcac ctatcgcggc cgtcaggttc tgacgcccaa ggaagactag
     3001 gtctttcagg cgcttggccg tttcggcggt cgaccgacat tcgaacggcg cgcgatgcta
     3061 tcgcgcgccg tttttatgcg ttgtactccg gccgttcgca actggagctt gcccgatgat
     3121 tcgtcgcgcc accgttctga cctccttcgc cgtcctcgcg ctcgccgcct gcggccagcc
     3181 caagcccgcg gagcaggccg aggctcctgc cgcacccacg accgcgtctg ccgaatcacc
     3241 tcccgctgac cccaatctcg tgaccgccga gggcatcggc ccgattcgca tcggccagac
     3301 catcggcgag gttcaagcgc tctcgggcac atcggatacg gcgatagcct cgccggacgg
     3361 atgcaacatc ttccatccct cacgtgcgcc cgccggggtc ttcgtcatga cggagatggg
     3421 ccgcgtcagc cgggtcaccc tgcgtgacgg ctccaccgcg aagaccgaca agggctttgg
     3481 cgtcggggcg gacgcctccg ccatcaaagc gtcttacg
//
LOCUS       DASVTM010000063         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_538164,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000063 DASVTM010000000
VERSION     DASVTM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538164"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..588)
                     /locus_tag="VF633_01630"
     CDS             complement(280..588)
                     /locus_tag="VF633_01630"
                     /inference="COORDINATES: protein motif:HMM:NF039302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5961 family protein"
                     /protein_id="HEX8469787.1"
                     /translation="MGQALRRQDVWDEIPEAAEITPAPTRPAPERRFAVHAADDGRHR
                     WETVWAVSHDDAAIGFVERHHPTVGDDGEVTVVVTDCESGHEHCFRIDLDTGDTAPCA
                     "
     gene            complement(651..1706)
                     /locus_tag="VF633_01635"
     CDS             complement(651..1706)
                     /locus_tag="VF633_01635"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8469788.1"
                     /translation="MSPILLDAAPARTRPGLLTSSAAYKPFRYPWAYEFWKRQQQVHW
                     MPEEVPLGEDCKDWASNLNDGERNLLTQIFRFFTQSDIEVADNYMEKYGRVFKPTEVK
                     MMLSSFANMETIHIAAYALLLETIGMPESEFGAFLDYQAMRDKHDFMQKFGVETNADI
                     ARTLAMFGGFTEGLQLFASFAMLMNFPRFNKMKGMGQIVSWSVRDESLHCEGIIKLYH
                     AFAHETGAVTKAVADDIVDCCRTVVGLEDRFIDLAFEMGPVQGMTPEDIKTYIRFIAD
                     WRLRQLGLPEVYGVKENPLPWLQSLLSGVEHANFFEARSTEYSKAATRGQWQGPDGVW
                     STFDRIKARAAAESAHH"
BASE COUNT          277 a          581 c          570 g          299 t
ORIGIN      
        1 cggaaccgga tggtcatcca gaagccctac acgctcgaaa acgtcgcccg cgccatggac
       61 gacctgctca cgacgccgtc ggtgagcacc gcggcaggct agctagacca aaccggttta
      121 gatgaactca ccgctgtcat cccggccgga gccgcaggcg gagagccggg atccaggagg
      181 aacggccacc agcccctgga tcccggctcg cgctgcgcgc gtccgggatg acaaaggcca
      241 tcgactccac ttggtccggc cccgctccag cagccgcgct caggcgcagg gcgcggtgtc
      301 gccggtgtcc aggtcgatgc ggaagcagtg ttcgtggccg ctctcgcagt cggtgacgac
      361 caccgtcacc tcgccgtcgt cgccgacggt cgggtgatgg cgctcgacga agccgatcgc
      421 cgcgtcgtcg tggctgacgg cccagaccgt ctcccagcgg tgccggccgt cgtcggccgc
      481 gtgcacggcg aagcgacgct ccggcgcggg cctggtcggc gcgggcgtaa tctccgcagc
      541 ttccgggatc tcgtcccaga cgtcctggcg tctcagtgct tgacccatag ggtgacctcc
      601 tgccgcggat caacgccgcc gggcggaaaa ggattcacgt tgcgttcgcg tcagtgatgc
      661 gctgattcgg ccgcggcgcg cgccttgatc cggtcgaagg tgctccacac gccgtccggc
      721 ccctgccact gaccgcgcgt ggcggccttg gagtattcgg tggagcgcgc ctcgaagaag
      781 ttcgcgtgct cgaccccgct gagcaggctt tgcagccacg gcagcgggtt ctccttgacg
      841 ccgtagacct ccggcagacc cagctgccgc aggcgccagt ccgcgatgaa gcggatgtac
      901 gtcttgatgt cctcgggcgt catgccctgc acgggaccca tctcgaacgc caggtcgatg
      961 aagcggtcct ccagcccgac caccgtgcgg cagcagtcga cgatgtcgtc cgccaccgcc
     1021 ttggtcaccg cacccgtctc atgggcgaag gcgtggtaca gcttgatgat cccctcgcag
     1081 tgcaggcttt cgtcccgcac cgaccagctg acgatctggc ccatgccctt catcttgttg
     1141 aagcgcggga aattcatcag catcgcgaaa ctggcgaaca gctgcagccc ctcggtgaag
     1201 ccgccgaaca tggcaagcgt ccgcgcgatg tcggcgttgg tctcgacgcc gaacttctgc
     1261 atgaagtcgt gcttgtcgcg catggcctgg tagtcgagga acgcgccgaa ctcgctttcc
     1321 ggcatgccga tcgtctcgag cagcagcgcg taggccgcga tgtggatggt ctccatgttg
     1381 gcgaaggatg acagcatcat cttcacctcg gtcggtttga agacgcggcc gtacttctcc
     1441 atgtagttgt cggcgacttc gatgtccgac tgggtgaaga agcggaagat ctgcgtcagc
     1501 aggttgcgct cgccgtcgtt caggttcgac gcccagtcct tgcagtcctc gccgagcggc
     1561 acctcctcgg gcatccagtg cacctgctgc tgccgcttcc agaactcgta cgcccaagga
     1621 tagcggaacg gcttgtaggc ggcgctggag gtcagcaggc cggggcgcgt ccgggcggga
     1681 gcggcgtcga gcaggatcgg ggacatggcg ggcgctccgg aatcgac
//
LOCUS       DASVTM010000064         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_698877,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000064 DASVTM010000000
VERSION     DASVTM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698877"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF633_01640"
     CDS             complement(<1..555)
                     /locus_tag="VF633_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX8469789.1"
                     /translation="MSHILTLVFGAGVLGVLYGLAQTAVLMKAGTGNDKMREIAAAIQ
                     EGASAYLKRQYATIGMVGVVILIAAWLLIGPYAAVGFVVGAVLSGAAGYAGMLISVRA
                     NVRTAQAASESLAKGLNLAFRSGAITGMFVAGGALMGVSGYFAVLVMHMGLEPTSREV
                     IDGLVALGFGASLISIFARLGGGIF"
     gene            complement(750..1205)
                     /locus_tag="VF633_01645"
     CDS             complement(750..1205)
                     /locus_tag="VF633_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469790.1"
                     /translation="MRRRDLIAAGTAVALSGPIVTSASFASASDKKAEGEPAVAPAIN
                     LPPVGLPVIVNGRIRNYVFISLKLHLGGEATAETVRARLPFLKNALVRTAHTAPFTMP
                     DDWALLSENKIKAAVMAIAGVVAPHSVTACEVLTQVPRRRTGMKPASSL"
     gene            complement(1260..>2233)
                     /gene="thiL"
                     /locus_tag="VF633_01650"
     CDS             complement(1260..>2233)
                     /gene="thiL"
                     /locus_tag="VF633_01650"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX8469791.1"
                     /translation="SGEFETIQKLLAPLAHPEWGRGLVDDVAVLPSRPGYDLVLTKDA
                     MVEGVHFLAADPLDTVAQKLLRVNLSDLAAKGAEPFGYLLACHWSARCGWPEREAFVE
                     GLARDQKTFGIALLGGDTVVTPGPASFSVTLLGWARAGQVVSRSGARIGDAVFVTGQI
                     GDGWLGLQAAQGGLALEQARIDALAEHYRRPVPQLDFSSVVRDMATASLDVSDGLVAD
                     LGHIAEASGVCIEIDLELTPLSMAGQAWFDSRVDPQAAFEQLATGGDDYEIAFTAAAS
                     DEAALRREAERRHVRLTKLGRVVAGKGVAVRYGHKLIDIAKTGWTHG"
BASE COUNT          412 a          765 c          708 g          348 t
ORIGIN      
        1 gaagatgccg ccgccgagac gcgcgaagat ggagatcagc gaagcgccga agcccagagc
       61 caccagaccg tcgatgacct cacggctcgt gggctccagg cccatatgca tgaccagaac
      121 ggcgaaatag ccggagacgc ccatcagggc gccgcccgcc acgaacatgc cggtgatggc
      181 gccggaccgg aacgcgaggt tcagcccctt ggccagactt tccgacgcgg cctgcgccgt
      241 ccggacgttc gcgcgcaccg agatcagcat cccggcatag cccgccgcgc ccgaaagcac
      301 cgcgccgacg acgaagccga cggccgcgta aggaccaatc agcagccagg cggcgatcag
      361 gatgaccacc ccgaccatac cgatggtggc gtattgccgc ttcaggtaag ccgaggcccc
      421 ttcctggata gcggcggcga tttctcgcat cttgtcgttg ccggtgccgg ccttcatcag
      481 cacggccgtc tgggcgaggc cgtacagcac ccccagcaca ccagccccga agaccagcgt
      541 aagtatatga ctcattggcg tttccatgcc cttgcaaaag gcgcccggct ccttggcgcg
      601 gcgggccttt tggcctctat ccgccttccg gctcccccgg gcgctacgac cgcagaactg
      661 cgatcactcg ggaattgccc ggcgaccgtg ccgcatgcgt cgagacgacg caaccatgct
      721 cttcgaaggg gtctggacga gcgccgttgc tagagggaag aggcgggctt cattccggtg
      781 cgacggcgcg gcacctgggt gagaacttcg caggccgtaa ccgagtgcgg cgcgaccacg
      841 ccggcgatcg ccatcaccgc cgccttgatc ttgttttcgc ttagcaacgc ccagtcatca
      901 ggcatggtga agggcgcggt atgagccgtg cgcaccaggg cgttcttgag gaagggcagc
      961 ctggcgcgca cggtctcggc cgtagcttcg ccacccagat ggagcttcag gctgatgaag
     1021 acatagttgc ggatgcgacc attgacgatc accggcaaac ccacgggagg aagattgatc
     1081 gcgggggcta ctgcgggttc gccctcagcc ttcttgtcgc tggcggaagc gaaggaagcg
     1141 gacgtcacga tcggccctga cagggcgacg gcggttccgg cggcgataag gtctctgcgg
     1201 cgcatggtcg aaccgtagcg gcgaccggtt aggattgcgt ttcggagcaa ttggacgatt
     1261 tagccgtggg tccaaccggt cttggcgata tctatcagct tgtggccgta gcgcaccgcg
     1321 acgcctttac cagccaccac gcgtcccagc ttcgtcagtc gaacatggcg gcgctcggcc
     1381 tcgcgtcgca gggcggcttc gtccgaggcg gcagcggtaa aggcgatctc gtagtcatca
     1441 ccgccggtgg ccagttgctc gaacgccgcc tgtgggtcga cccggctatc gaaccacgcc
     1501 tgtccggcca tggacagcgg cgtcagttcc agatcgatct cgatgcagac cccggaagcc
     1561 tcggcgatgt gtccaaggtc cgcgaccagc ccgtccgata cgtcgagaga cgcagtcgcc
     1621 atgtcccgaa ccacgctgga gaagtccagc tgcggaacag gtcggcgata gtgttcggcc
     1681 agggcgtcga tccgcgcttg ctccaacgca agcccgcctt gcgccgcctg cagtcccagc
     1741 cagccgtcgc cgatctggcc cgtgacaaaa accgcatcgc caatgcgcgc accgctccgg
     1801 cttacgacct gtccggcccg cgcccagccc agaagcgtaa cggagaacga ggccggcccc
     1861 ggcgtgacga ctgtgtctcc gcccagcagc gcaatcccga acgtcttctg gtcgcgcgcc
     1921 agtccctcca cgaaggcctc gcgttcgggc caaccgcagc gggcggacca atggcaggcc
     1981 agcagatagc cgaacggctc tgcgcccttg gccgccagat cggacaggtt cacgcgcaac
     2041 agcttctgcg ccaccgtatc cagcggatca gcggcgagga aatggacgcc ctcgaccatc
     2101 gcgtccttgg tcagcaccag atcataaccc ggccgcgacg gcagcacggc gacatcgtcg
     2161 accagccccc tgccccactc ggggtgagcc aggggcgcca gcagtttttg gatcgtttcg
     2221 aactcgccgg aca
//
LOCUS       DASVTM010000065        17656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_60303, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000065 DASVTM010000000
VERSION     DASVTM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60303"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VF633_01655"
     CDS             complement(<1..795)
                     /locus_tag="VF633_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8469792.1"
                     /translation="MTAVTTDDEAKGPGLATVVGASAAGTAFEWYDFFIFGSLTTIIA
                     RHFYADIGEATSYILALLTFGVGFVVRPLGALAFGWFGDRTGRKTTFLVTITLMGAAT
                     VAIGLLPDYGMAGIAAPILLILMRIIQGFALGGEYGGAAIYVAEHAPAKTRGLLTSYI
                     NTTAALGLILALVVILSTRAILGTEAFNEWGWRVPFWLSAALLAISLWIRVRLHESPA
                     FVKMAAESKVRQAPFKESFLTWKNGRWVLVALVGIMFAQGAVWYASY"
     gene            complement(977..1117)
                     /locus_tag="VF633_01660"
     CDS             complement(977..1117)
                     /locus_tag="VF633_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX8469793.1"
                     /translation="MRKLFVLFAAAAALTTAACNTIAGAGRDTSAAGQAVTGAANEVK
                     GN"
     gene            complement(1163..2845)
                     /locus_tag="VF633_01665"
     CDS             complement(1163..2845)
                     /locus_tag="VF633_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8469794.1"
                     /translation="MAIALLPILVLGAIQAQADFRDQGADRRSALQLAAARSAATTRA
                     RLDSTTILLEALGAEATGPRCTPRLAALNERLADYDALYRTSATGDIVCGSGPDSFPA
                     AMVRTGPWFQRLSAGEPFVVTRFASPGSPRPGLVVASRLEQPMGRFAGAMVAVIPLNH
                     LQPDVTDPALPEGAQAALTDGQGQILTATDVRAFQLKGDALGGWVDRARSAETAVFEG
                     NDAAGQHRVYAGAALAGRDVYILLSAPAPGLLSWARLNPIGSILLPFASWLAAFAAVM
                     LVTERVVVRWLDYLERVAAIYARGRFSVRPVQAANAPSEIRLLAQTLDELAENITTRD
                     TMLTESLAEKDALMREIHHRVKNNLQIISSLLSMQQRALTDAPAKAALGDTRQRISAL
                     ALIYRTLYQSENIRYADAREFLTELVGQLVATEAMRGPVVSSSVVADSLVVDPDKLAP
                     LALWLVEAVTNAQKHAFAGRGGDLQVRFTVDGDKSTLEVQDDGPGPDERAVKAGVGRT
                     LMGAFAKQLRGEAEILRGANGGTIARMVFATPEAVVPSDPGDLVGTARTTSR"
     gene            complement(2959..3579)
                     /locus_tag="VF633_01670"
     CDS             complement(2959..3579)
                     /locus_tag="VF633_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8469795.1"
                     /translation="MSVSSSRSSAPPKPASADDVGFKRELVTLIPHLRAFARTLTGDP
                     TAADDLAQDAMMKAWDARASYQMGTNMKAWTFMILRNQFYSEKRRSWRQSQLDQEAAE
                     RTLVAVDDPEAPVALDELRMALNTLPEEQREALILVGAGGFAYEEAAEICQCAVGTVK
                     SRVSRARRALLATLERGGYARDGRAAGDAMRSILADADRLSGARQG"
     gene            complement(3576..3776)
                     /locus_tag="VF633_01675"
     CDS             complement(3576..3776)
                     /locus_tag="VF633_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEX8469796.1"
                     /translation="MIDTPDSRRKAAPTGEAGLEEARLRQQAIGVKLRHMFDEVVNEP
                     VPDEFLAILRRADDKSSGGSQG"
     gene            4022..4816
                     /locus_tag="VF633_01680"
     CDS             4022..4816
                     /locus_tag="VF633_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8469797.1"
                     /translation="MSLLARLAPHLPYVRRYARALTGDQATGDNYVRVALEALAAGER
                     QLSTEMTPRVALYQVFHAIWSSTGAQLEDQSGIEGRDDASRRLMRIAPRSRQAFLLTA
                     LEGFTPSEAAQILSADVHDVERLIGEAQAEIDSELATDVLIIEDEAIISADIESLVKE
                     LGHRVIATATTHDEAVDAVARHRPGLVLADIQLADGSSGIDAVKDILKRFDVPVIFIT
                     AFPERLLTGERPEPTFLITKPFQPETVKAAISQALFFHPSRQKEAA"
     gene            complement(4881..5252)
                     /locus_tag="VF633_01685"
     CDS             complement(4881..5252)
                     /locus_tag="VF633_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469798.1"
                     /translation="MTPEKTDPHSEAADAVHDLRPVDADLVRSARPGKRILLVLGGGL
                     ILVVIGFLAIWGPGGGALSRTNANDGDQAVDTAAFQGDALNPPTADAPTTATGAAQPR
                     PTGEAPNVNAPTVPSNRAPAH"
     gene            5601..6620
                     /locus_tag="VF633_01690"
     CDS             5601..6620
                     /locus_tag="VF633_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8469799.1"
                     /translation="MPINRQWVLRQRPQGLIKDGDLELIESPTPDLKDGEVLIRTVYL
                     SLDPTNRTWMNDAVGYLPPVGLGDVMRGLTLGVVEETKSDRFKAGDVVTTAMGGWADY
                     GVVPDVGASRVHRAPGLPLTANMSVMGMTGLTAYFGVTDVLRPEPGQVMVISAAAGAV
                     GSIAGQIAKQLGAHVIGIAGGSDKCRWLVEELGFDAAIDYKGEDVGAALDRLAPNGVD
                     LNFENVGGDIMVAVWNRLNIGARMAVCGMVSAYNATKRPPSPDLSRLITHRMTMQGFL
                     VLDYAPRAREMIAEMGPWLADGQVKWKVHVDNGLEGALTSLNRLFTGDHDGKLLVRVS
                     EEPAE"
     gene            6631..7038
                     /locus_tag="VF633_01695"
     CDS             6631..7038
                     /locus_tag="VF633_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HEX8469800.1"
                     /translation="MSESLHLHFEDLDNGQVLQLGAIAVDEAAVEAFAERFAPGWDVA
                     YGAPDAMVYALWSRLAAEKSSGWAQTKVLAVDGLRFMRNPPPGELLRGRMTVMGKDPV
                     GDEKGIVIAQHDLLDEGGRLVFSCLTRALFVRR"
     gene            complement(7152..7943)
                     /locus_tag="VF633_01700"
     CDS             complement(7152..7943)
                     /locus_tag="VF633_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX8469801.1"
                     /translation="MTDTMQSDGQLAGTVLFYSKPEPLSAELHGKLGANPVDKPYAFV
                     AQTHVVPLTVTEFAPAALSYPVVFLGDNKQPVAVMGLRQSENLYVDATGEFRAEAYIP
                     AYARRYPFVFANDDQQQRMILCIDRDAPFIVEGGQVPLFDNGQPSAYVNQAMEFCNNF
                     EQERQRTDSFVALLTELDLFHTREAVFTPRDEQGNPGEPQKLADYFAVSEDKLKALPA
                     EKLAELRDNGALGQIYAHLVSLLGWDRLIALAFQRAAQMPTAANA"
     gene            complement(8153..10207)
                     /locus_tag="VF633_01705"
     CDS             complement(8153..10207)
                     /locus_tag="VF633_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469802.1"
                     /translation="MRKTSALSTRTALLAFAGWTALAGVAAAQTAPTPDAADTVDDVV
                     VTASPYGVSQRASNIATTVLDEQALAVAPAQSLGDMLAGQPGLRSTGFAPGASRPVIR
                     GLSGPRVQVLTNGIGMIDASSVSPDHAVATDPAEASRIEVVRGPSTLIYGGSAIGGVV
                     NIIDSRIPTEIPNGGLSGVISTQASSVDDGKSIFARLTAGTGHFAFNIDGVKRSTDDY
                     DIPSPARSARLAAQDGEPRGAGGTQPNSYTELEAWGVGGAYIFDKGYFGVSYKDTNSE
                     YGTVAEPTVFIQLHQTREDVRGEYNFDAGPFKTIRGNYGHAEYTHTEFEGPGEPGTIF
                     NSDGQEGRIDLIQRERSMSGGGTWNGAIGVQGLSRTFEAIGDEAFVPTSDIEEAGIYT
                     IQRLDRGPFGFEGGLRYDRHMVTATPIGDVTEIEREFDNVSASLAGFIRPAPGLFLGL
                     SLAHNERAPSEVELFADGLHIATTAYETGDRTLDTEKVNTLEGTVHYDAGKVSADLHV
                     YGSKYDGFIDERPTGATFTSEEGEEFPIFAFVQTDATFYGFEAEGSYALWETGNRKLA
                     LEAAADYVNAETDLGPAARIPPWSLTSRLAWTSKPFDASIEVRHVAEQDRVADLELPT
                     DDYTLLNLAAAWRPLADRNVTVFAEARNLTDEEAREHVSFLKDIAPMPGRNFRVGVAY
                     RF"
     gene            10316..10813
                     /locus_tag="VF633_01710"
     CDS             10316..10813
                     /locus_tag="VF633_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX8469803.1"
                     /translation="MASACDHDHHHDRPGVDAIGIALSAAEGRCVAEGERMTAPRRRV
                     LELLLTAGEPVKAYDLIARYGEDGQAAKPPTVYRALEFLERKGMAHRIASISAYVACT
                     AGNDAEALPHAAAFLICDCCGATEELLAPTGQTFSLAADAAGYAIERTTIEGHGRCPA
                     CRVAA"
     gene            10860..11744
                     /locus_tag="VF633_01715"
     CDS             10860..11744
                     /locus_tag="VF633_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8469804.1"
                     /translation="MDAALLTPPRRLSVPVDNRWGAGQMSVLDFGDAKRPVDLVYVHA
                     NGFNAATYRTLLAPLSASLRIWAPDLRGHGRTTLPTPVVGRWSWADHRDDLIALLDTI
                     DGPPVVMSGHSMGGTSSLLAAAERRDKVSQLVLLDPVIWTRAWVTAFKFPLLRSLPRR
                     IPLAKGALRRRRVFESRDQALIAYRGRGAFKGWPEMALAHYLSDGLVETDEGLVLACA
                     PEWEASNYAAQSHDPWKALARLDRPVRILKAETASTCHVASDPRRQITLETVAGGTHF
                     FPMLKPEIARDALFDAAV"
     gene            complement(11741..12808)
                     /gene="prfA"
                     /locus_tag="VF633_01720"
     CDS             complement(11741..12808)
                     /gene="prfA"
                     /locus_tag="VF633_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX8469805.1"
                     /translation="MRLPQARLDQVLDRFHQVEARMGSVTDGQEIVRLSKEHSEMKPV
                     ADAVQALAKARAEIEDLASMAEDPEMAAMVADELETLNATLPDMERDVALLLAPRDAD
                     ENASAVLEVRAGTGGDEAAIFAGDLFRMYSRYASTRGWRVELDSATEGDAGGYKEIIA
                     TVTGDGVFGRLKFESGVHRVQRVPATESQGRIHTSAATVAVLPEVEDVEIEIHDKDIR
                     IDTYRASGSGGQHVNTTDSAVRITHLPTGIMVTSSEKSQHVNRDKAMKNLRVRLYDMQ
                     RQAKDLVRSDARKSQVGSGDRSERIRTYNYPQGRVSDHRINLTLHSLPQIMEGDIDPL
                     LNALIAEDQAARLADLEAEFN"
     gene            13129..13905
                     /locus_tag="VF633_01725"
     CDS             13129..13905
                     /locus_tag="VF633_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8469806.1"
                     /translation="MNDFLIFLLVGFLAQSVDGALGMAYGVISSTVLLAFGVPPAAAS
                     ASVHAAEVFTTAASAGSHAVNKNVNWALFAPLALGGVVGGILGAFVLTSIDGNLLKPW
                     ITLYLAVMGITIVWRAWRGTRPKDFSKKLAGPLGVAGGFFDAIGGGGWGPTVTTTLVG
                     SGENARMSIGTVNTAEFFVTSAISATFLAALLTGHWQQADGLMTHATAVAGLVVGGLL
                     AAPMAGVITRIAPTKVLTYAVGVIVLLVAGYQALRLFGVV"
     gene            complement(13963..14496)
                     /locus_tag="VF633_01730"
     CDS             complement(13963..14496)
                     /locus_tag="VF633_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469807.1"
                     /translation="MILRKLAIAGALASVMGLAACATPSNPGNMTLPASAGLTATAGD
                     LGYHSVTKVDVSGGKETNPLWTAQVSSEAFKKALEDSLALAGYMGTDGPPMQVQANLV
                     NLRQPFAGFDLSVTSEVRYTVTRDGRSVFDETVSATGTASMGESFMAVERLRMANEKS
                     IQENIKAFLTRFRARAH"
     gene            complement(14493..14954)
                     /locus_tag="VF633_01735"
     CDS             complement(14493..14954)
                     /locus_tag="VF633_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HEX8469808.1"
                     /translation="MFSVSIARFGVRALVLAGVSLSLPACATITRGSSQEFTVQSQPP
                     GATVRTSNGFECAATPCTFRMQRKSAFTATFELDGYATETRQVESKMAGAGAAGMAGN
                     ILVGGIIGGVVDASSGAMNDLTPNPLLVILQTPAQRAANPAPAPAPSGAAQ"
     gene            complement(15063..16199)
                     /gene="ispG"
                     /locus_tag="VF633_01740"
                     /gene_synonym="gcpE"
     CDS             complement(15063..16199)
                     /gene="ispG"
                     /locus_tag="VF633_01740"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX8469809.1"
                     /translation="MSDHSHIRPWRHIERRKSRQIMVGSVAVGGDAPISVQSMTNTLT
                     SDANATIGQIRELEEAGADIVRVSCPDEESTAAFKTIARAAKVPLVADIHFHYKRGIE
                     AAQAGAACLRINPGNIGNASRVRDVIQAAKDYGCSMRIGVNAGSLERELLEKYGEPCP
                     DAMVESALNHARILQDHDFHEFKISVKASDPFLTVAAYQQLAEAIDCPLHLGVTEAGP
                     LRTGTIKSAIGMGNMLWAGIGDTIRVSLAADPVEEIKVGFDILKSLGLRHRGVNIIAC
                     PSCARQGFNVIDTVVLLEEKLAHISTAMSLSIIGCVVNGPGEALYTDVGFTGGGKGSG
                     MVYLNGKIAHKLDNALMVDEIVGLVEAKAAELDAAREAELQAAE"
     gene            complement(16255..17400)
                     /locus_tag="VF633_01745"
     CDS             complement(16255..17400)
                     /locus_tag="VF633_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8469810.1"
                     /translation="MGSAVMALDTGAASDEFRSHADWKDLAPSIADAATLGSGLRATR
                     EASGRSLAELTVATRVHTRYLIAMEEGDFSALPSRVFAIGYVRAYAEALGLDEQLAVE
                     RFKRESPDGSVPLQAPVGVAFQDVRRYSPRVIGVIVAIAVAVVAWNVFQRVNLMHAPQ
                     PSDIASVPESWSLGDVPGQPAVAIGAPQPAPPDQTTPALYITPGLEAQLTGIDPTDTE
                     AVAAAAAAQAPPVQAAFNPRGAIYGAAATASQVVLQAKKAAALVVRMGDGRVLFARQL
                     AAGESWRAPTGVAATVDVSDPTAFDVYLNGEHGGGLATVLTPLSQLNSRADQLARQTA
                     AEVSARTASAQRAAQAATDQAARRTGQAMQAGAGVAQQGLNMAVSPR"
BASE COUNT         2987 a         5563 c         5886 g         3220 t
ORIGIN      
        1 atagctggcg taccagaccg cgccttgggc gaacatgatg ccgacgaggg caacgagaac
       61 ccagcggccg ttcttccagg tcaggaagct ttccttgaac ggcgcctgac gcaccttgct
      121 ctcggcggcc atcttcacga aggccgggct ttcgtgcagg cgaacccgga tccacagcga
      181 tatggccagc agggccgccg aaagccagaa cgggacgcgc caaccccact cgttgaaagc
      241 ttcggtaccc aggatggcgc gggtgctcag aatcacgacc agcgcgagga tcaggcccag
      301 agccgcagtc gtgttgatgt aggaggtcag cagtccgcgc gtcttcgctg gcgcgtgttc
      361 ggcgacgtag atggccgcgc cgccgtactc cccgccgagc gcgaaaccct gaatgattcg
      421 catcaggatg agcaggatcg gcgcggcgat cccggccatg ccgtagtcgg gcaacaggcc
      481 gatggcgacg gtcgccgcgc ccatgagggt tattgtgacg aggaaggtgg tcttgcggcc
      541 tgtgcggtcg ccgaaccagc cgaacgccag cgcgccgagc gggcgtacga cgaaaccgac
      601 gccgaaggtc agcagtgcga ggatgtagct cgtggcctcg cctatgtcgg cgtagaaatg
      661 gcgggcgatg atcgtcgtca gcgatccgaa gatgaagaag tcgtaccact cgaaggccgt
      721 accggcggcg gacgctccga cgaccgtcgc aagaccaggg cccttggctt cgtcatcggt
      781 ggtgactgcg gtcatcgtgt tctcccggag gcctctgcgg gccatctctc gggagacact
      841 agcgaggcgg ttgggttcag gaaagcgcgg ctaccccgcg atatccagca aaaaccccgc
      901 cggtgggggg accgacgggg ctctgcagcc tcggtgaccc gttcaggccg ccgatatggc
      961 taactccctt gcgggattag ttgcccttaa cttcgttggc ggcgccggtc acggcctgac
     1021 cggctgcgct cgtgtcgcgg ccagcgccgg cgatagtgtt gcaagcagcg gtcgtcaggg
     1081 cggcggcggc ggcgaaaaga acgaacagct tgcgcatggg tctactccgg aaggcgcgac
     1141 gattcgcgca ggaactgtcg gatcaacgcg atgtggttct ggcggttccg actaaatctc
     1201 cgggatcgct gggaacaacg gcttcggggg tggcgaagac cattcgggca atggttccgc
     1261 cgttcgcccc gcgcagtatt tcggcttcgc ctctcaattg tttggcgaaa gcgcccatca
     1321 gagtccgtcc gacgcctgcc ttgaccgccc tttcgtccgg gccggggcca tcgtcctgaa
     1381 cctccagcgt gctcttatct ccgtcgacgg tgaagcgaac ctgaaggtcg ccgccgcgcc
     1441 ccgcgaaggc gtgtttctgg gcgttggtga cggcctcgac cagccacaag gcgagcggcg
     1501 ccagcttgtc tggatcgacg accagactat cggccaccac tgaactggaa actaccgggc
     1561 cgcgcatggc ctcggtcgcg accagttgcc cgaccagttc agtcagaaac tcgcgggcgt
     1621 ctgcatagcg gatgttttcg gattggtaga gggtgcgata gatcagggcg agagccgaga
     1681 tgcgctggcg cgtatcgccc agcgcggcct tggccggagc gtcggtgagc gcgcgctgct
     1741 gcatcgacag gagcgacgag atgatctgca agttgttctt cacccggtgg tggatctcgc
     1801 gcatcagcgc gtccttctcc gccaggctct cggtcagcat cgtgtcacgg gtggtgatat
     1861 tctcggccag ctcatcgagc gtctgtgcca gaagccggat ctccgatggg gcattggcgg
     1921 cctggaccgg ccggacagag aaccggcctc gcgcgtaaat ggctgcgacc cgctccagat
     1981 aatccagcca acgcacgaca acccgttctg tcaccagcat cacggcggcg aaggctgcca
     2041 gccatgatgc aaacggcagc aggatcgatc caatgggatt caaccgggcc cacgagagca
     2101 ggccgggggc aggagctgaa agcaggatat aaacatcacg tcccgcgagg gcggcgcccg
     2161 catagacgcg gtgttggccc gccgcatcgt tgccctcgaa aacggctgtt tctgcagagc
     2221 gggcgcgatc gacccaacct ccaagcgcgt cgcccttgag ctgaaaggcg cgcacgtcgg
     2281 tggcggtgag aatttggccc tgcccgtccg tcagggcggc ctgtgcgcct tcgggcaggg
     2341 ccggatctgt tacgtcgggt tgcagatggt tcaggggaat caccgccacc atggcgcccg
     2401 cgaaacgtcc catcggctgt tcgagccgtg atgcgaccac cagtcccggc cgtggcgaac
     2461 cgggcgaggc gaaacgcgtg acgacgaagg gttcaccggc gctcaagcgc tggaaccacg
     2521 gaccggtacg gaccattgcg gcggggaacg agtccggtcc cgagccacag acgatgtcac
     2581 ccgtggcgct ggtccgatac agcgcgtcgt aatcggccag tcgctcgttc agggcggcga
     2641 gccgcggcgt gcaccggggg cccgtcgctt cggcgcccaa ggcttcgagc aggatcgtcg
     2701 tgctgtccag ccgagctcgg gttgtcgcgg ccgaacgagc cgccgccaac tgcagggctg
     2761 atctgcggtc ggcgccctga tcgcggaagt cggcctgggc ctggatcgcc cccagcacca
     2821 ggatcggcaa aagggcaatg gccatggcca cacccaaccg gaagcggatg ctctggaatc
     2881 ttctggatcc cagtggcggc ttgcctccta ggcgtttgag atcgcgaccc agggagtggc
     2941 cgatacgcac cacgggcttc aaccctgcct ggcgccgctc agacggtcag catcggcaag
     3001 gatcgaacgc atggcgtccc cggcggctcg accgtcgcgg gcgtatccgc cacgctccag
     3061 agtggccagc agggcacgac gggcgcggct cacccggctt ttgactgtcc caaccgcgca
     3121 ttggcagatc tcagctgctt cttcataggc gaagccgccg gcgccgacca gaatgagcgc
     3181 ttcgcgctgt tcctcgggca gggtgttcag ggccattcgc agttcgtcca atgcgacggg
     3241 agcctccgga tcgtcaacgg cgacgagcgt gcgctctgcg gcttcctggt cgagttgcga
     3301 ctggcgccac gagcggcgct tctccgagta aaactggtta cgcaggatca tgaaggtcca
     3361 ggccttcatg ttggtgccca tctgataact ggcccgggcg tcccaggcct tcatcattgc
     3421 gtcctgcgcc agatcgtccg ccgcggtcgg gtcgccggtc agggtgcggg cgaatgcgcg
     3481 tagatgcggg atcagcgtca ccaattcacg cttgaagcct acgtcgtccg ccgaagccgg
     3541 cttcggcggc gcggacgatc gcgaggatga gacactcatc cctggctccc gcctgaggat
     3601 ttgtcgtcgg cgcggcgcag gatcgcgagg aactcgtccg gaaccggctc gttgacgacc
     3661 tcgtcgaaca tgtgacgaag cttaacgcct atggcttgct gccggagccg ggcttcttcc
     3721 agtccggcct caccggtggg agcggcctta cgccgggagt caggagtatc gatcatggac
     3781 aattggcccg ccacccgttg cacgcattca aggatgcacc gatctttgcg atcagcgtca
     3841 ttatggaccc cgacaacgtc ggcgaacgga cacggttcct acgcgacatg cgaataatgg
     3901 tctaggttca gcctcgagat ggaaccgtgg cgctgatgat acgtttcggt ggcactctcg
     3961 cgcctgctgc gttgtcggta tttggtcgcg tcgttctgtt atgtcgggga atattctgaa
     4021 aatgagcctt ctggccagac tcgcgccaca ccttccttac gttcgtcgtt acgcccgtgc
     4081 cctgaccggc gaccaggcga ctggcgacaa ttatgtccgc gtcgccctgg aggctttggc
     4141 cgcaggcgag cgccagcttt caaccgagat gacccctcgt gtggctcttt atcaggtgtt
     4201 tcacgccatc tggtcctcga caggcgcaca actggaagac cagagcggca tcgaaggccg
     4261 tgacgacgcc tcgcgtcgtc tgatgcggat cgccccgcgt tcacgccagg cattcttgct
     4321 gactgcactt gaaggcttca ccccttcgga agccgcccaa attctttcgg cagacgtcca
     4381 tgacgtcgag cggctcatcg gtgaagccca ggccgagatc gactccgaac tggctacgga
     4441 cgttctcatt atcgaggatg aggccataat ctccgccgac atcgagagtc tggtcaagga
     4501 gctcggccac agggttatcg ccacggcgac gactcacgat gaggctgtcg acgccgtcgc
     4561 acgtcatcgt ccgggtctgg tcctcgctga catccagctg gccgacggtt cgtcaggcat
     4621 agacgctgta aaggacatcc tgaagcgctt cgacgtgccg gtaattttca tcacagcctt
     4681 ccctgagcgg ctgttgaccg gcgagcgccc ggaaccgacc ttcctgatca ccaagccgtt
     4741 ccagccggaa acggtaaagg cggctatcag tcaggctctg ttcttccatc cttcacgtca
     4801 gaaggaagcg gcgtaagcct ctttccaagc cggagttaag ggccgtcgga cacagtccga
     4861 cggccctttc gtttggcctt ttagtgggcg ggtgcgcgat tggacggaac agtcggagcg
     4921 ttgacgttgg gagcctcacc tgtcggtcgg ggctgggccg ctccggtggc cgtcgtcggt
     4981 gcatcagccg tcggaggatt caacgcgtcg ccctggaagg ccgcggtatc gaccgcctga
     5041 tcaccatcgt tggcgttcgt ccgggacaag gccccgcccc ctggtcccca gatggcgagg
     5101 aacccgataa cgaccagaat cagtcctccg ccgagaacca gcagtatacg cttgccggga
     5161 cgggcgctgc gaacgaggtc cgcatcgact ggtctaaggt catggacagc gtcagcggcc
     5221 tcggaatggg gatcagtctt ctcgggcgtc atcggtgtct ccgttcgcgc atacccgggg
     5281 tcgggctctc tgaacgcaaa cgttgtttgc acctgcgccg ttccgttcgt cccacttcat
     5341 ccaccgcgga agaaaacgtt aaaaatctct cgcccgaagg aacgcctttc aagagtcatc
     5401 gttatcaatc tgcggaggcg gcgacgcccc cccgacttga aactggcgaa agccagtttg
     5461 tcgcctccgc gcctcattct cgatgatgcc acttcacggg cggatctttg atccgtccgt
     5521 ttttctttgc actcctctcg acagccgccg cagctcggtc atcatgggtc cacaaaagaa
     5581 accatctcgg gagacgttct atgccgatca atcgtcagtg ggttctgcgt cagcgacctc
     5641 aagggctgat caaggacggt gacctcgaac tgatcgagag ccccacgcct gatctgaaag
     5701 acggcgaggt tctgatccga accgtttacc tgtcgctgga cccaaccaac cggacctgga
     5761 tgaacgacgc cgtcggctat ctgccgccgg tagggctggg cgatgtcatg cgcggtctga
     5821 ccctgggcgt tgtcgaggag accaaatcgg acaggttcaa ggcaggcgac gtcgtcacca
     5881 ccgcgatggg cggctgggcg gactacgggg tcgtgccgga tgtgggcgcg tcgcgggtcc
     5941 accgcgcgcc gggcctgcct ctgacggcca atatgtcggt gatgggcatg accggactga
     6001 cggcctattt cggcgtcacc gatgtgctga ggcccgagcc ggggcaggtc atggttatct
     6061 cggcggcggc gggcgcagtc ggctccatcg ccggtcagat cgccaaacag ctaggcgctc
     6121 acgtcatcgg catcgccggc ggatccgata aatgtcgctg gttggtcgag gagctcgggt
     6181 tcgacgcggc catcgactat aaaggcgagg acgtcggcgc ggctctcgat cggctggccc
     6241 cgaacggcgt cgacctcaat ttcgagaacg tcggcggcga catcatggtc gcggtctgga
     6301 atcggctgaa catcggcgcc cgaatggcgg tctgcgggat ggtctccgcc tataacgcga
     6361 ccaaacgccc gccgtcgccg gacctcagcc gcctgatcac tcaccggatg acgatgcagg
     6421 gttttctggt gctcgactac gcgccacgcg ctcgcgagat gatcgccgaa atggggccat
     6481 ggctggcgga cggccaagtc aagtggaagg tccacgtcga taacggcctc gaaggcgcgc
     6541 tgacctcatt gaaccggttg ttcaccggcg atcatgacgg caagctgttg gttcgcgtgt
     6601 ccgaagagcc ggccgagtaa ggaccagacc atgagcgaaa gcctgcacct ccatttcgag
     6661 gacctcgaca acggccaggt gctgcagctc ggcgccattg cagtcgacga ggcggcggtg
     6721 gaggctttcg ccgaaaggtt cgcgcccggc tgggacgtcg cctacggcgc gcccgacgcc
     6781 atggtctacg ccctgtggtc gcggctggcg gcggagaagt cgtccggctg ggcccagacc
     6841 aaggtcctcg ccgtcgacgg ccttcgcttc atgcgcaacc cgccaccggg cgaactgctg
     6901 cgtggccgca tgaccgtgat gggcaaggat cctgtcggcg acgaaaaagg catcgtcatc
     6961 gcccagcatg atctgctcga tgagggcggg cggctggtct tcagctgcct gactcgggcg
     7021 ttgttcgtga gaagataggc agtaggcagt aggcagtagg cagtaggcag taggcagtag
     7081 gggaggcgga atggcggtga gctctcgctt ctactgcctg ctgcctgacc cctactgcct
     7141 ccttcttcct atcaggcgtt agccgcagtc ggcatctgcg cggcgcgttg gaacgcgagt
     7201 gcgatcagac ggtcccagcc gagcagcgac accaggtggg cgtagatctg gccgagggcg
     7261 ccgttgtcgc gcagttcggc aagcttctcg gccggcaggg ccttcagctt gtcttccgag
     7321 accgcgaaat agtcagccag cttttgcggc tcgccggggt tgccctgctc gtcgcgtggc
     7381 gtgaagacgg cttcgcgggt gtggaacagg tcgagttcgg tcagcagggc gacgaagctg
     7441 tcggtgcgct gacgctcttg ttcgaagttg ttgcagaact ccatcgcctg attgacgtag
     7501 gccgagggct ggccgttgtc gaacagaggc acctggccgc cttcgacgat gaatggagca
     7561 tcgcggtcga tgcaaaggat catccgctgc tgctggtcgt cgttggcgaa gacgaacgga
     7621 taacggcggg cgtaggcggg gatataggcc tcggcccgga actcgccggt tgcgtcgacg
     7681 tagaggttct cactctggcg caggcccatc acggcgaccg gctgcttgtt gtcgccgagg
     7741 aagacgacgg gataggacag ggcggccggg gcgaactcgg tcacggtcag cggcacgacg
     7801 tgcgtttgag ccacgaacgc atagggcttg tcgactggat tggcgcccag tttgccgtgc
     7861 aactcggccg aaagcggctc gggcttgctg tagaacagga cagtgccggc cagttggccg
     7921 tcggattgca ttgtgtcggt catggacgcc aaggtctctg gaaaaactga aagtcgcgcc
     7981 ttctagcgga agccggcgtc ggcggcaaac gcgaccatcc cattgggcga gtctcgctcc
     8041 ccctcagggg agcgagatca cttcttcgtc gagcagtgag cggtcatcga gagcgatagc
     8101 gccccaaacc tgcgctcaag cagcaagccg ccgcgcggcg agcgatagcg ccctagaacc
     8161 tgtaagccac gccgacgcgg aagttccggc cgggcatcgg agcgatgtcc ttgaggaagg
     8221 agacgtgttc gcgcgcctcc tcatccgtca agttcctggc ctcggcgaag accgttacgt
     8281 tgcgatcggc cagcggacgc caggcggcgg cgaggttcag cagggtgtag tcgtcggtcg
     8341 gcagttcgag atcggcgacc cggtcctgtt cggcgacgtg acgcacctcg atgctggcgt
     8401 cgaacggctt ggaggtccag gccagacggc tcgtcaacga ccacggcggg atgcgcgctg
     8461 ccggaccgag gtccgtctcg gcgttgacgt agtcggccgc cgcttcgaga gccagcttgc
     8521 ggtttccggt ctcccacagg gcgtaggagc cctcggcttc gaagccgtag aaggtagcgt
     8581 cggtctggac gaaggcgaag atcgggaatt cctcgccctc ttccgaggtg aaggtcgcgc
     8641 cggtcgggcg ctcgtcgatg aagccgtcgt atttcgatcc atagacgtgc aggtcggccg
     8701 aaaccttgcc cgcgtcgtaa tggaccgtac cttcgagagt gttgaccttt tcggtgtcga
     8761 gcgtccggtc gccggtctca taggcggtgg tggcgatgtg caggccgtcg gcgaacagct
     8821 cgacctcgga tggcgcgcgc tcgttgtgag cgaggctcag gccgaggaaa agaccgggcg
     8881 cagggcggat gaagcccgcc agcgaggccg agacgttgtc gaactcgcgt tcgatctcgg
     8941 tgacgtcgcc gatcggtgtg gccgtcacca tatggcggtc atagcgcagc ccaccttcga
     9001 agccgaacgg accccggtcc agtcgctgga tcgtgtagat ccccgcttcc tcgatatcgc
     9061 tggtcgggac gaaggcttcg tcgccgatgg cttcgaacgt ccgcgaaaga ccctgaacgc
     9121 cgatggcgcc gttccacgtc ccaccgcccg acatcgaccg ttcgcgctgg atcaggtcga
     9181 tgcgaccttc ctgaccgtcg gagttgaaga tcgtgccggg ctcgccgggg ccttcgaact
     9241 cggtgtgggt gtattcggca tggccatagt tgccccggat cgtcttgaac ggacctgcgt
     9301 cgaaattgta ctcaccgcgc acgtcctcgc gcgtctggtg caactggatg aagacggtcg
     9361 gctcggcgac ggtgccgtac tcgctgttgg tgtccttgta ggacacgccg aaataaccct
     9421 tgtcgaagat gtaagcgccg ccgacgcccc aggcttcgag ctcggtatag gagttaggct
     9481 gagtgccgcc cgcgccgcgc ggttcgccat cctgcgctgc gaggcgggcc gagcgggcgg
     9541 gtgagggaat gtcatagtcg tcggtcgagc gcttcacgcc gtcgatgttg aaggcgaaat
     9601 ggccggtccc ggcggtcagg cgggcgaaga tcgacttgcc gtcgtcgacg ctggacgcct
     9661 gggtcgagat cacgcccgac aggccgccat tcgggatctc ggtcggtatg cggctgtcga
     9721 tgatattgac caccccgccg atggccgagc caccatagat gagggttgag gggccgcgta
     9781 cgacctcgat gcggctggcc tcggcgggat cggtggccac ggcatggtcg ggcgagaccg
     9841 aggaggcgtc gatcatgccg atgccattgg tcaggacctg aacgcgcggg ccggacagtc
     9901 cccggatcac cggacggcta gccccgggag cgaagccggt cgagcgcagg ccgggctgac
     9961 ctgccagcat gtcgccgagc gattgggcag gcgcgacggc cagagcttgt tcatccagca
    10021 ctgtggtagc gatgttggag gcgcgttggc tgaccccgta cggcgaggct gtaacgacca
    10081 catcgtcgac ggtgtcggcg gcatccggcg tcggcgcggt ttgcgcagcg gcgacaccgg
    10141 caagggctgt ccagccggca aaagcgagta gtgcggtgcg ggtggaaagg gcggaagttt
    10201 tgcgcatgag agacctcggc gtcggggaag acactaatct gttatgagat aacatcacag
    10261 atcgtcaagc cctgcttggt tgcgaagcgg acgttcacgc cctagctgtg tctccatggc
    10321 ctccgcttgc gatcacgatc accatcacga ccgccccggc gtcgacgcca tcggcattgc
    10381 gctgtccgca gcggaggggc gctgcgtggc cgagggcgag cgcatgaccg cgccccgccg
    10441 ccgggtgctg gagctgttgc tgacggctgg agagccagtg aaggcctatg acctgatcgc
    10501 ccgctatggc gaggacggac aggccgccaa gccgccgacg gtctatcgtg cgctggagtt
    10561 tctagagcgc aagggcatgg cgcaccggat cgcctcgatc agcgcctatg tcgcctgcac
    10621 ggccggcaac gacgccgaag cccttccgca cgcggcagcc ttcctgatct gcgactgctg
    10681 tggcgcgacc gaagagttgc tggcgccgac cggccagacc ttctccctcg ctgcagacgc
    10741 cgccggctat gcgatcgaac gcaccacgat cgagggtcat ggtcgctgtc ccgcttgccg
    10801 ggtggctgca tagggtttcg ccgctggcga aagcgatgcg ccatccctag attggatcga
    10861 tggacgccgc ccttctgacc ccgccccgtc gtctgtccgt tccggtcgat aatcgctggg
    10921 gcgccgggca gatgtccgtg ctggatttcg gcgacgcaaa gcgcccggtc gatctggtat
    10981 acgtccacgc caacggcttc aacgccgcca cctatcgcac cctgctggcc ccgctttcag
    11041 cgtcgttgcg catctgggcc ccagacctgc gtggtcatgg ccgcacgacc ctgcccacgc
    11101 ccgtcgtcgg tcgctggagc tgggccgatc accgcgatga cctgatcgcc ctgctcgaca
    11161 ccatcgacgg tccgccggtg gtgatgtcgg gtcactcgat gggcggcacg tccagcctgc
    11221 tggcggctgc cgagcggcgc gacaaggttt cacaactggt gctactcgac cccgtcatct
    11281 ggacgcgcgc ctgggtcacg gctttcaagt ttccgctgct gagaagcctg cctcgccgca
    11341 ttccattggc aaaaggcgca ctgcgtcgtc gccgcgtgtt tgagagccgc gatcaagcgt
    11401 tgatcgccta tcgcggacgc ggcgccttca agggctggcc cgaaatggcg ctggctcact
    11461 atttgtccga cggtctggtc gagaccgacg aaggtcttgt gctcgcctgt gcgcccgagt
    11521 gggaagcgtc caactacgcc gcccagtcac acgatccgtg gaaggctctg gctcggctgg
    11581 atcgccccgt gcgcatcctg aaggccgaga ccgcctcgac ctgccacgtc gccagcgacc
    11641 cgcgccgtca gatcacgctc gagaccgtgg ctggcggtac gcatttcttc ccgatgctga
    11701 agcccgagat cgctcgcgac gccctgttcg acgccgctgt ttagttaaat tccgcttcca
    11761 gatcggcgag acgcgcggcc tgatcctcgg cgatcagggc gttcaacagg ggatcgatat
    11821 ccccctccat gatctgcggc aggctgtgca gggtcaggtt gatgcggtga tcgctgaccc
    11881 gtccctgcgg atagttgtag gttcggatgc gctccgaccg gtcgcccgag ccgacctgcg
    11941 acttgcgggc gtcggaccgg accagatcct tggcctggcg ctgcatgtcg tacagacgca
    12001 cgcgcaggtt cttcatcgcc ttgtcgcggt tgacgtgctg ggatttctcc gacgacgtca
    12061 ccatgatgcc ggtcggcagg tgggtgatgc ggacggctga atcggtcgtg ttgacgtgct
    12121 gaccgcccga gccggaggcg cggtaggtgt cgatgcggat gtccttgtcg tggatctcga
    12181 tctcgacgtc ctcgacctcg ggcagcaccg cgactgtcgc cgcggaggtg tggatgcgcc
    12241 cttgcgactc ggtcgccggc acccgctgga cacggtggac gccgctctcg aacttcagcc
    12301 ggccgaagac gccgtcgccg gtgaccgtgg cgatgatttc cttgtagccc ccggcgtcgc
    12361 cctcggtggc gctgtccagt tcgacccgcc agcctcgcgt cgaggcgtag cgcgaataca
    12421 tgcggaacag atcgccggcg aagatggcgg cttcgtcccc gcccgttccg gcgcggactt
    12481 ccagcacggc cgaagcgttc tcgtcggcgt cgcgcggcgc cagcaacagg gcgacatcgc
    12541 gctccatatc cggcagcgtc gcgttaagcg tttccagctc atcggcgacc atggcggcca
    12601 tctcgggatc ctcggccatg ctggccagat cctcgatttc cgcgcgcgcc ttggccaggg
    12661 cctggacggc gtcggcgacc ggcttcatct ccgagtgctc cttggacagg cgcacgatct
    12721 cctgaccgtc cgtgaccgag cccatacgcg cctccacctg atggaaacgg tcgagcacct
    12781 gatcgagccg ggcctggggc agtcgcaagg gagaagtcgc ctgttcttga ggaggcgcgg
    12841 tcttactccg ggatgcggcc tcctgtcgag gaagggcgtt gcggcgcccg gatcgcgctc
    12901 gggcgtcacg ttcgcgtcag ccccggttac catcccggcc tcatgaacta gatgtttcaa
    12961 gctgtagccg gagaaccctc agatggtcgc gcgcctgcaa gagtgaaatg tgcgaccggc
    13021 gctgaaaggc gaggccggag aattactccc tcgagtgacc atgatttgtc gctttaccct
    13081 gtgccgggca cgttcgagaa cgcggctccg ctttccgcca gagacgccat gaacgacttt
    13141 ctgatcttcc tgctcgtggg cttcctggcg caaagcgtcg atggggccct cggcatggcc
    13201 tacggtgtaa tttcatcgac ggtgctgctg gcgttcggcg tgccgcctgc agccgcgtcc
    13261 gccagtgtcc atgcggcgga agtcttcacc acggcggcct cggccgggtc ccatgcggtc
    13321 aacaagaacg tcaactgggc gctgttcgcg ccgctggcgc tgggcggggt ggtcggcggc
    13381 atactcggcg ccttcgtcct gaccagcatc gacggcaatc tgctcaagcc ctggatcacc
    13441 ctgtatctgg cggtcatggg gatcacgatc gtctggcgcg cctggcgcgg tacgcgtccc
    13501 aaggatttct cgaagaagct ggcggggcct ttgggcgtcg ccggtggctt cttcgacgct
    13561 atcggcggcg gtggctgggg cccgaccgtg acgaccaccc tcgtcggctc cggcgagaac
    13621 gcgcgcatgt cgatcggcac tgtcaacacc gcggagttct tcgtcacctc ggcaatttcc
    13681 gcgaccttcc tcgccgccct cctgaccggc cactggcagc aggccgacgg cctgatgacc
    13741 catgcgacag cggttgcagg tctggtcgtt ggcggccttc tggcggctcc catggccggc
    13801 gtcatcaccc gcatcgctcc gaccaaggtc ctgacctacg ccgtcggcgt catcgtcctg
    13861 ttggtcgccg gctatcaggc gctgcggctg ttcggggtgg tttagagacg gggatgacgg
    13921 gaccagcttt ctccgatccc gtcgcgtcca cgtcctgacg gctcagtgcg cgcgagcgcg
    13981 gaagcgggtc aggaaggcct tgatgttttc ctggatcgac ttttcgttgg ccatccgcag
    14041 gcgctccacg gccatgaagg attcgcccat actcgccgtt ccggtcgccg aaactgtctc
    14101 atcgaaaacc gaccgaccgt cgcgggttac ggtgtagcgc acctcgctgg tgaccgagag
    14161 gtcgaagccg gcgaagggtt ggcggaggtt taccagattg gcctgaacct gcatcggcgg
    14221 cccgtccgtt cccatgtaac cggccagagc cagagagtcc tcgagggcct ttttgaacgc
    14281 ttcgctggaa acctgagcgg tccacagagg attggtttcc ttgcccccgc tgacatctac
    14341 cttggtcacc gaatggtaac ccaggtcgcc agccgtagcg gtcaggcctg ccgacgcagg
    14401 cagggtcatg ttgcccgggt tggagggggt ggcgcaggcg gcgaggccca tgacggaggc
    14461 aagagctccg gcgatcgcaa gtttgcgcaa gatcattgag ccgctccgct cggagcgggc
    14521 gctggggcag ggtttgcagc gcgctgagca ggagtttgga gaatgaccaa caaggggttg
    14581 ggtgtcaggt cattcatcgc gccggagctg gcgtcgacca ctccaccgat gatgcctccg
    14641 accagaatgt tgcctgccat accggcagcg cctgcgcccg ccatcttgct ctcaacctga
    14701 cgcgtctcgg tcgcatagcc gtccagttcg aaggtagccg tgaaagcgct tttgcgttgc
    14761 atccggaagg tgcagggcgt ggcggcgcac tcgaagccat tggacgtgcg gaccgtggct
    14821 ccgggcggct ggctttgaac ggtaaactcc tggctagagc cgcgcgtgat ggtcgcgcag
    14881 gctggcagag aaagcgaaac gcccgcaagc acgagcgcgc gcacgccgaa gcgcgcgatt
    14941 gaaactgaaa acattgaagc ccctgagtcg ataaagttca ccccccggcg aactggccgc
    15001 aacttggcat tgcgctcttg gccggtcaac caagccgtga agcttgagac ccgcagggtg
    15061 cgttattcag ccgcctgaag ctcagcctca cgcgccgcgt ccagttccgc ggccttcgcc
    15121 tcgaccaggc cgacgatctc gtcgaccata agagcgttgt cgagtttgtg agcgatcttg
    15181 ccgttcaggt agaccatgcc cgaacccttg ccgccgccgg tgaagcctac gtcggtgtag
    15241 agggcctcgc ccgggccgtt gacgacgcag ccgatgatag acagcgacat ggcggtcgag
    15301 atgtgagcga gcttttcttc cagcaagacc accgtatcga tgacgttgaa gccctgacgg
    15361 gcgcacgacg ggcaggcgat gatgttgacc ccccgatggc gcagacccag cgacttgagg
    15421 atgtcgaacc cgaccttgat ctcctcgacc ggatcagcgg cgagggagac gcggatggtg
    15481 tcgccaatac cggcccacag catattgccc atgccgatcg cggacttgat cgtgccggtg
    15541 cgtaacggcc ccgcctcggt gacgccgagg tgcagcggac agtcgatggc ctcggccagc
    15601 tgctgatagg cggcgacggt caggaaggga tcggatgcct tgaccgaaat cttgaactcg
    15661 tggaagtcgt gatcctgaag gatgcgggcg tggttgaggg cgctctcgac catcgcatcc
    15721 gggcagggct cgccgtattt ctccagcagt tcccgctcca gcgacccggc gttgacgccg
    15781 atccgcatgg agcagccgta gtccttggcg gcctggatca cgtcgcggac gcgcgaggcg
    15841 ttgccgatat tgcccggatt gatcctcagg caggcggcgc cggcctgagc cgcctcaatg
    15901 ccgcgcttgt aatggaaatg gatgtcggcg accaaaggca ccttggcggc ccgggcgatc
    15961 gtcttgaatg ctgccgtcga ctcttcgtcg gggcatgaga cacggacaat gtccgcgccg
    16021 gcctcctcga gctcacggat ctggccgatc gtcgcattgg cgtccgaggt cagggtgttg
    16081 gtcatcgact ggacgctgat cggcgcgtcc ccgcccacgg ccacggaccc caccatgatc
    16141 tggcggctct tgcggcgctc gatatggcgc cacggacgga tatgcgaatg gtcgctcata
    16201 gttgccacta gataggcgct acatcatggc atggccatga ctggcgcgat gatatcaacg
    16261 cggcgagact gccatattca aaccctgctg ggcgacaccg gcccccgcct gcatggcctg
    16321 tccggtccgg cgcgctgctt gatccgtagc ggcctgagcg gcgcgttggg ctgacgcggt
    16381 ccgggccgag acctcggcgg cggtctgacg ggccagttga tcggcacggc tgttgagctg
    16441 gctcaggggc gtcaggacgg tggcaagacc cccgccgtgc tccccgttca ggtagacatc
    16501 gaacgcagtg ggatcggaga cgtcgacggt ggccgcgacg cccgtcgggg cgcgccagct
    16561 ctcacctgcg gccagttgtc gggcgaacag gacgcggccg tcccccatgc gcacgaccag
    16621 agcggcggcc tttttcgcct gcaacacgac ttgcgaagcg gtggcggcag cgccgtagat
    16681 ggcgccgcgt ggattgaagg cggcctgaac cggcggggcc tgggcggcgg ctgcggcggc
    16741 gacggcttcc gtgtcggtgg ggtcgatacc cgttagctgg gcctccaggc cgggcgtgat
    16801 gtagagggca ggcgttgtct ggtccggcgg agcgggttgc ggagcgccta tggcgactgc
    16861 gggctgtccg gggacgtcgc cgaggctcca gctctcggga accgaagcga tgtccgacgg
    16921 ctggggcgca tgcatcaggt tgacgcgctg gaatacgttc caggcgacga ccgcaaccgc
    16981 gatggcgact attaccccga tcacacgagg cgagtagcgc cgtacgtcct gaaatgcgac
    17041 cccgaccggc gcctggagcg gcaccgagcc gtccgggctt tcccgcttga agcgttcgac
    17101 ggccagttgt tcgtcaaggc cgagcgcctc ggcataagcg cgcacatatc cgatggcgaa
    17161 aacgcgcgag ggcagggcgg agaaatcgcc ttcttccatc gcgatcagat agcgagtgtg
    17221 cacacgggtc gcgaccgtca gttcggcgag tgatcggcct gaagcttcac gggttgcgcg
    17281 cagtccggat ccaagagtcg ccgcgtcggc gatggagggc gcgaggtcct tccagtcggc
    17341 gtgcgaacgg aactcgtcgg aggcagcccc cgtatccagc gccatgacgg cagaccccat
    17401 actcggtgcg ttcgacccgg ttgaagcggg gcggcggtcg tgcatcgtac tcgaccagtg
    17461 acagagcgat accacatcgg tttcgccgct ctggcctccc ccccggaggc catttgtgct
    17521 cgtcttgtgt ataactcatt tccgctcgtc gttcaacgca gtgttaatca cgacggatac
    17581 tcgcccgacc ggtgcggcgg ggcctcgcca ggcctcgagt atcggtgata tcgcgtcata
    17641 ccgggacacg tcagac
//
LOCUS       DASVTM010000066         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_486081,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000066 DASVTM010000000
VERSION     DASVTM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486081"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VF633_01750"
     CDS             <1..1247
                     /locus_tag="VF633_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX8469811.1"
                     /translation="EVGLALRYLRAKKKEGGVALIAIISFFGIMLAVMALIVVLSIMS
                     GFRSELLGRMLSFNGHMYVTGPVLTSPDRNAALARIKAVPGVISVSPLVEAQALIQAS
                     GQTTGAYVRGLRPEDLGSMSYVSSSLSPEAKASFGKGAYGGDRILIGQALADQLGLRV
                     NDPITLYSPTGADSAFGNLGGNRKTYFVGGTYSSGAADYDRSFIFMPQEQAQLFFGKE
                     GVWDVIELKVAKADEVASYTEAVRNAAGRGSLVTDWTLRLAAFWGALKVERVAMSIIL
                     GMVVAIAALNIISGIVMLVKNKTRDIAILRTIGASPSSILRVFFMAGAMIGISGMLVG
                     LILGLLFCLNIGAIQHFLEFVFQTQLFNADVYMLDSIPAKVDPMDVVWVTLWSVFMSC
                     VASLIPSWLATRIDPVEALRYE"
     gene            1240..1941
                     /locus_tag="VF633_01755"
     CDS             1240..1941
                     /locus_tag="VF633_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8469812.1"
                     /translation="MSDPVLSLRNIVRTYTTGRGDLTVLKGVDLDVYPGEIVGLIGPS
                     GSGKSSLLHAAGLLERPTEGVVMIDGEDVGTLDERARTRIRLNRIGFVYQFHHLLPEF
                     DARDNVALPIRIAGHKEAEARAMAEESLASLGLADRVLHQPAQMSGGEQQRVALARAL
                     ANKPRLLLADEPTGNLDPATSQTVFEALHALVKNTGVAALIATHNMELAGHMDRVFAL
                     KDGLLVERPAESHAY"
     gene            1954..>2140
                     /locus_tag="VF633_01760"
     CDS             1954..>2140
                     /locus_tag="VF633_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469813.1"
                     /translation="MTEAIQKEFEIAREAYAEDDRVTARNSYARGAALARELNKPLLR
                     AFALRHLADIDRDADRPA"
BASE COUNT          343 a          769 c          641 g          387 t
ORIGIN      
        1 gggaagtcgg cctcgccctc cgctacctgc gcgccaagaa gaaggaaggc ggcgtcgccc
       61 ttatcgccat catctccttc ttcggcatca tgctggcggt aatggccctg atcgtggtgc
      121 tcagcatcat gtcgggcttc cgctccgaac tgctcggccg catgctgtcc ttcaacggcc
      181 atatgtatgt caccgggccc gtgctgacat cgccggatcg caacgccgct ctggcccgca
      241 ttaaggctgt cccgggcgtg atcagcgtca gccccctggt cgaggcgcaa gcgctgattc
      301 aggccagtgg ccagacgact ggcgcctacg ttcgcggcct tcgtcccgaa gaccttggct
      361 cgatgtccta cgtctcgtcc agcctctcgc ccgaggccaa agccagcttt ggcaagggcg
      421 cctacggcgg tgaccggatc ctgatcggtc aggcgttggc cgaccagctc ggcttgcgcg
      481 tcaacgaccc gatcaccctc tattccccga cgggcgccga cagcgccttc ggcaatctgg
      541 gcggcaaccg gaagacctat ttcgtcggcg gcacctattc gtctggcgcc gcagactatg
      601 accgcagctt catcttcatg cctcaggaac aggcgcaact cttcttcggc aaggaaggcg
      661 tctgggacgt catcgagctc aaggtcgcca aggccgatga ggtcgcatcc tacaccgagg
      721 ccgtgcgcaa cgcggcgggc aggggatcgc tggtcaccga ctggaccctg cgcctcgccg
      781 ccttctgggg tgcgctcaag gtcgagcggg tggccatgag catcatcctc ggcatggtcg
      841 tggccatcgc cgcgctcaac atcatctccg gcatcgtcat gctggtgaag aacaagacgc
      901 gcgacatcgc catcctgcgc accatcgggg ccagcccgtc gtcgatcctg cgcgtcttct
      961 tcatggccgg cgccatgatc ggcatctcgg gcatgctggt cggtttgatc ctcggcctgc
     1021 tgttctgtct caacatcggg gcgatccagc atttcctcga gttcgtgttc cagacccagc
     1081 tgttcaacgc cgacgtctat atgctcgact ccatacccgc caaggtggac ccgatggacg
     1141 tcgtctgggt caccctgtgg tccgtcttca tgtcctgcgt cgccagcctg atcccgtcgt
     1201 ggctcgcgac ccggatcgat ccggtcgagg cccttcgcta tgagtgatcc tgttctgagc
     1261 ctgcggaata tcgtccgcac ctacacgacc ggtcggggcg acctgaccgt gctcaagggc
     1321 gtcgatctgg atgtttatcc gggtgagatc gtcggcctga tcgggccttc gggctccggc
     1381 aagtcatcgc tgctgcacgc cgccggctta ctggagcgtc cgaccgaggg cgtggtgatg
     1441 atcgacggcg aggatgtcgg cacgctcgac gagcgcgccc gcacccgcat ccgcctgaac
     1501 cgcatcggct tcgtctacca gttccaccac ctgcttcccg agttcgacgc ccgcgacaac
     1561 gtcgccctgc cgatccgtat cgccggtcac aaggaagccg aggcccgcgc catggcggag
     1621 gaatctctgg cgtcgctcgg tctcgccgac cgtgtcctgc accagccggc ccagatgtcg
     1681 ggtggcgagc aacaacgtgt ggcccttgcc cgcgcgctcg ccaacaagcc gcgcctgctg
     1741 ctggccgacg aaccgaccgg caacctcgat cccgccacca gccagaccgt gttcgaggcc
     1801 ctgcacgcct tggtcaagaa caccggcgtc gccgccctga tcgcgaccca caacatggag
     1861 ctggccggtc acatggaccg cgtcttcgcc ctcaaggacg gtctgctggt cgagcgcccc
     1921 gccgagagcc atgcctacta aggacccgac gccgtgaccg aagcgatcca gaaagagttc
     1981 gagatcgccc gggaggccta tgccgaggac gaccgggtca ccgcgcgcaa cagctatgcg
     2041 cgcggcgccg cccttgcgcg cgagctcaac aagcccctcc tgcgcgcctt cgccctccgc
     2101 cacctcgccg acatcgaccg cgacgccgac cgccccgccg
//
LOCUS       DASVTM010000067         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_378900,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000067 DASVTM010000000
VERSION     DASVTM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378900"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1400
                     /locus_tag="VF633_01765"
     CDS             198..1400
                     /locus_tag="VF633_01765"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX8469814.1"
                     /translation="MTVTFSDIQAAQSRIAGQVDRTPVRHSRRLSQLTGAEVWVKYDN
                     LHFTGSFKERGALNRLLQLTPDEMRRGVVAASAGNHAQALAYHGGRLGVPVTIVMPEG
                     TPFTKADGTRSHGATVVIKGADFTASTQEAHRFRDEEEMVFISAFDDEGIVTGQGVCA
                     IEFLEDAPDLDVLIIPIGGGGLIAGCSIAAKAMKPDIRIFGVETEMYPSFAARRRGEM
                     PKCGGATIAEGIAIKAVGDIPYALADPLVEDVLVCAEEDFEKAVALYVTLEKTVAEGA
                     GAGGLAALLRYPEKFAGLKVGLVLCGGNIDARMLAVVLNREMVRERRLIVYRILGDDR
                     PGMLSAMAAVIGGLGGNIIDVVHNRLALDVPAKGAEFDIMVETRDSAHADEIGQALKD
                     QGYALRMG"
     gene            1381..1920
                     /locus_tag="VF633_01770"
     CDS             1381..1920
                     /locus_tag="VF633_01770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8469815.1"
                     /translation="MRFGWVKAATAFAMGAAILTTAACNRTPPVSAETLAKNAADAAA
                     FMEKNKSAEGVQTLPSGLQYKVLTSGPSGAASPDRNDLVRVDYEGALTDGTVFDSSFE
                     RGVPLAIHVDEVVPGWTEALQRMKKGDEWMLYLPPALGYKDQNAGDIPPNSALVFRVK
                     LLDIAPVPGAGRAIGAAQG"
     gene            complement(1837..>2554)
                     /gene="queG"
                     /locus_tag="VF633_01775"
     CDS             complement(1837..>2554)
                     /gene="queG"
                     /locus_tag="VF633_01775"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX8469816.1"
                     /translation="FLGVILSAAEIEPDAAETEHCGSCTACLDACPTNAFPAPFQLDA
                     RRCLSYLTIEFPGAWPVEFRAATGSRIYGCDDCLAVCPWNKFAEASKEIRLQARDALE
                     QPPLAELATLDDAAFRALFSKSPIKRIGRDRFLRNVLYAVGNSGDAALVATAERLLDD
                     ASPLVRGAAVWALGRLDAVAFGRARGERLAGEADEGVKREWALLPLREKDVSALRRPN
                     RASRTGHGGDVEELDPEHQR"
BASE COUNT          445 a          847 c          847 g          415 t
ORIGIN      
        1 gccaaggcga gaaaggtcag tgaggcggcc aaggcagggc gggcgaaacg ggtttgcgtg
       61 gacatggcgt ttcctttctg cgcgtcacgc tcctttgccc cgtgaagggg tcggcgcgat
      121 cggctctcag tcataccccc aacgcgcccc ccaaggtcaa ggttgcgggc gcttgctggc
      181 tgagccaaaa catggcgatg accgtgacat tttcagacat ccaggctgcc cagtcccgaa
      241 ttgcgggaca ggtggaccgg acccccgtcc gccattcccg ccgcttgtcg caactgaccg
      301 gcgctgaggt ctgggtgaag tacgataatc tgcatttcac cggctcgttc aaggagcgag
      361 gcgccctgaa ccgtctgctc cagctgacac ccgacgagat gcggcgcggc gtcgtcgcgg
      421 cctcagccgg caatcatgcg caggctctgg cctatcacgg cggtcggtta ggtgttcccg
      481 tgaccatcgt catgcccgag ggcaccccgt tcaccaaggc cgatggcacc cgcagccacg
      541 gcgcgaccgt ggtcatcaag ggggcggact tcaccgcctc gacgcaggag gcgcaccggt
      601 tccgcgatga ggaggagatg gtcttcatct ctgccttcga cgacgagggc atcgtcaccg
      661 ggcagggcgt gtgcgcgatc gagttcctcg aggatgcgcc ggatctcgac gtcctgatca
      721 tccccatcgg cggcggaggg ctgatcgccg gctgttcgat cgcggccaag gcgatgaagc
      781 ctgacatccg catcttcggg gtcgagaccg agatgtatcc gtcgttcgcc gcgcgccggc
      841 ggggtgagat gcctaaatgc ggcggtgcga ccatcgcgga agggatcgcc atcaaggcgg
      901 tcggcgacat tccttatgcg ctcgccgatc cgctggtcga agacgtgctg gtttgtgccg
      961 aggaagactt cgaaaaggcc gtggccctgt acgtcacgct cgagaagaca gtggccgagg
     1021 gcgcgggcgc cggcggtctg gcggcgttgc tgcgctatcc ggagaagttc gccgggctca
     1081 aggtggggct ggtgctgtgc ggcggaaata tcgacgcgcg gatgctcgcc gtggtgctta
     1141 atcgtgagat ggtccgtgaa cggaggctga tcgtttaccg cattctcggc gacgacaggc
     1201 cgggcatgct gtcggccatg gcggcggtga tcggcggcct cggcggcaac atcatcgacg
     1261 tcgtccacaa ccgtttggcg ctggatgtcc ctgccaaggg cgcggagttc gacatcatgg
     1321 tcgaaacgcg cgatagcgcc cacgctgacg agatcggtca ggcgttgaag gaccagggtt
     1381 atgcgcttcg gatgggttaa ggctgcgacg gcgttcgcga tgggcgccgc gatattgacg
     1441 accgccgcct gtaaccgcac cccgcctgtc tcggccgaaa ccctggccaa gaacgccgcc
     1501 gacgccgccg ccttcatgga aaagaacaag tcggccgagg gcgttcagac cctgccgtca
     1561 gggctccagt acaaggtgct gacctccggc ccgtcgggcg ccgccagccc cgaccgcaac
     1621 gatctggtcc gcgtcgacta cgaaggcgct ctgaccgacg gcacggtgtt cgacagctcg
     1681 ttcgagcgcg gcgtgccgct ggccatccac gtcgacgagg tcgtgcccgg ctggaccgaa
     1741 gccctgcaac gcatgaagaa gggcgacgag tggatgctct atctgccgcc tgcgctcggc
     1801 tataaggatc agaatgcggg tgatattccg ccgaactcag cgttggtgtt ccgggtcaag
     1861 ctcctcgaca tcgcccccgt gcccggtgcg ggacgcgcga ttggggcggc gcagggctga
     1921 cacgtccttc tcccgcaagg ggagaagggc ccactcgcgc ttcacgccct catcggcctc
     1981 gcccgccagc cgttcacccc gcgctcggcc aaacgccacc gcatccagtc gccccagcgc
     2041 ccaaactgca gccccgcgca ccaacggcga cgcatcgtcc agtaaccgct ccgccgtcgc
     2101 caccagcgcc gcatccccgc tgttgcccac cgcatagagg acgttcctca ggaaccggtc
     2161 gcgaccgatc cgcttgatcg ggctcttcga gaacagggcc cggaaggcgg cgtcatccag
     2221 cgtcgccaac tcggccagcg gcggctgttc cagcgcatcc cgcgcctgca gccggatctc
     2281 cttcgacgcc tcggcgaact tgttccacgg acagaccgcc agacagtcgt cacagccata
     2341 gatgcgcgaa ccggtcgccg cgcgaaactc gacgggccaa gcccccggga actcgatagt
     2401 cagatacgac aggcaccgcc gggcatcgag ctggaacggc gcaggaaagg cgttggtcgg
     2461 acaagcgtcg aggcaggcgg tgcaggagcc gcagtgctcg gtctcggccg catcgggctc
     2521 gatctccgcc gccgacaata tgacgccgag gaac
//
LOCUS       DASVTM010000068         6259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_911163,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000068 DASVTM010000000
VERSION     DASVTM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911163"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1121)
                     /locus_tag="VF633_01780"
     CDS             complement(135..1121)
                     /locus_tag="VF633_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX8469817.1"
                     /translation="MTVSSASRRFFKAASIKKLARTAVGAALGLALVAALVGSWAGPA
                     FAQATSAPVVSAISPAAGDGPALWVVKDVDSTIYLFGTIHVLRPETRWESAEVTAAFN
                     SALDIWFEISNPDDMAALIPLIKQYGVSPGTPLSSLLTPAELNELNAAAQTIGASAAQ
                     LDVFRPWYAGLTLSLAPLKKAGYDPNSGVELTLKKRAEAAGKPIHGFETLDKQVRLLA
                     NLPQDIQLEFLRSTLRDFDDAGTELDAMVAAWAAGDVAALDRVTNEDMKAEAPDIYKA
                     LLTDRNTGFADQIQTLLAGSGTAFVAVGAAHLAGDDSVQSMLSARGVEVSRE"
     gene            complement(1126..1314)
                     /locus_tag="VF633_01785"
     CDS             complement(1126..1314)
                     /locus_tag="VF633_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8469818.1"
                     /translation="MNNRLKVLRAERNWSQAHLAEELGVSRQTVNALETGRYDPSLPL
                     AFKIAKIFGQPIEAIFSE"
     gene            complement(1319..1804)
                     /locus_tag="VF633_01790"
     CDS             complement(1319..1804)
                     /locus_tag="VF633_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469819.1"
                     /translation="MSTMNVVTRKRVLQTVIGGVILAAVTVGGSVGLGYLVGHLSASA
                     EVGLDKAMMWGMGIFAVIVMAGAILVGTLWMRSIDEAAREAHKAAWYWGGSGGMAAGG
                     VLMILSFLPQAARISIPSVLSDRTDPAAYAVTGALALMTLMMIGYLIVWAWWWIVRSR
                     G"
     gene            complement(1879..3216)
                     /gene="rmuC"
                     /locus_tag="VF633_01795"
                     /pseudo
     CDS             complement(1879..3216)
                     /gene="rmuC"
                     /locus_tag="VF633_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
     gene            complement(3412..4047)
                     /gene="recR"
                     /locus_tag="VF633_01800"
     CDS             complement(3412..4047)
                     /gene="recR"
                     /locus_tag="VF633_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX8469820.1"
                     /translation="MAASAGPEIERLIALLAKLPGLGPRSARRAALQLLKRREQLLIP
                     LAAAMAETAEKVVSCSVCGTPDTRDPCSICSDPHRDNGLICVVEEAGSLWAMERSGAF
                     RGKYHVLGGLLSALDGIGPEALRVAELVGRARAGNATGGTNGEGGAVREVVLALPATV
                     DGQTTAHYLAERLSGSGVEVTSLARGVPVGGDLDWLDDGTIVQALRARRPA"
     gene            complement(4048..4377)
                     /locus_tag="VF633_01805"
     CDS             complement(4048..4377)
                     /locus_tag="VF633_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX8469821.1"
                     /translation="MKDLNSLMQQAQAMQQKLQDAQAKMADTTAEGTSGGGLVTIALK
                     GPGEITSVKIDDSLLVPGEGEILADLIVAAHADAKRKLDEINGELMRQAAGPLAGMNI
                     PGMPKLF"
     gene            4555..5166
                     /locus_tag="VF633_01810"
     CDS             4555..5166
                     /locus_tag="VF633_01810"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX8469822.1"
                     /translation="MAYDASTRDAVLDDNALAQLFTEARTRNAFSDRPVAPELLHRLY
                     DLTKFGPTAANTTPGRFLFITSSEAKARLTPFMSEGNKAKTMSAPVNVIVAYDGEFHE
                     QMPFLFPHNPDAKNWFGDEDARKIPGVLNSSLQAGYFMIAARALGLDVGPMIGFDAAG
                     VKAEFLADATWVPSFVINLGYGTDENLFPRFPRLPFDTAAKII"
     gene            complement(5214..>6259)
                     /locus_tag="VF633_01815"
     CDS             complement(5214..>6259)
                     /locus_tag="VF633_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX8469823.1"
                     /translation="LALIARAAEGSVRDGLSLLDQALVQGERGETVKTEVVRDMLGLA
                     DRSQTIALFEQIMGGRTPEALETFRTLYGYGADPIQVTNDLLEHCHAASVAKMLGPNA
                     TRLPADQAKTLAALGAAISAGTLSRTWQMLLKGLEEVRRAPSPADAVEMIIVRLAYAA
                     DLPAPEDALKKLQNGETLGGGSGGPTRGGGGGGGGSSVALAPRPAAPPALPDPQTFEA
                     TVALIGQRREISLQMDVQRYVKPVSFKPGAIVYESVEGAPIDLARRLASRLREWTGRT
                     WLIAANGQGGGQTLIEQEKSDRAQVRAEVEADPFVASVLAAFPGAVISDIRTLAPTIE
                     MPAIPDDPVDDED"
BASE COUNT         1095 a         2037 c         1994 g         1133 t
ORIGIN      
        1 tcctccgtgg gctgaccgag ccttcattcc tgccggtgtc tgccagacct cgttggccaa
       61 tccggccgct gaaggatggc ggtgccgcac agcttgtaaa gtaagggcag gctggaggct
      121 ggacagagcc gcatctactc ccggcttacc tccacgccac gggcagacag catcgactgg
      181 acactgtcgt caccggcgag gtgagcggct ccgacagcga cgaaggccgt gccggagccg
      241 gccagcaggg tctggatctg gtcggcgaag ccggtgttcc gatcagtcag gagcgccttg
      301 tagatgtctg gcgcctcggc cttcatgtcc tcgttggtga cgcggtccag agccgcgacg
      361 tcgccggctg cccaagcggc gaccatggcg tccagttctg tgcccgcatc gtcgaaatcc
      421 ctgagcgtcg agcgcaagaa ctcgagctgg atgtcctgcg gcaggttcgc gagaagccgg
      481 acctgtttgt cgagggtttc gaacccgtgg atcggttttc cggccgcctc ggcacgcttc
      541 ttgagcgtca gttcgacgcc ggagttcggg tcatagccag ccttcttcag cggcgcgagc
      601 gacagagtga gcccggcata ccacggacgg aagacgtcca attgggccgc gctggcgcca
      661 atggtctggg ctgcggcgtt gagttcgttg agctcggccg gcgtaagcag gctggacagc
      721 ggtgtgccgg gcgagacgcc gtattgcttg atcaggggga tcagggccgc catgtcgtcc
      781 gggttcgaaa tctcgaacca gatgtctaag gcgctgttga aagcggcggt gacctcggcc
      841 gactcccagc gggtttcggg tcgcagcacg tggattgtgc cgaatagata aatggttgag
      901 tcgacgtcct tgacgaccca cagcgcggga ccgtctcctg cagcggggct tatggcgctg
      961 actaccggcg cagacgtcgc ctgtgcgaac gctggccccg cccaggaccc caccagagcg
     1021 gcgactagcg ctagccccaa tgcggcgccg acagcggtcc gcgccagctt cttgattgat
     1081 gccgccttga agaagcggcg agaggcggac gagacagtca tgattttact ctgaaaagat
     1141 ggcttcgatg ggctggccga agatcttggc gatcttgaag gcgagcggca gggatgggtc
     1201 gtagcggccg gtttccagcg cattgacggt ctggcgcgaa acaccgagtt cttcggccag
     1261 atgggcctga ctccagttcc gttcggcgcg cagcaccttg aggcgattgt tcatcgggtc
     1321 agccccgcga cctgacgatc caccaccatg cccaaacaat gagatagccg atcatcatca
     1381 gcgtcatcag ggccagggct ccggtgacgg cgtacgccgc cgggtcggtt cggtcagaca
     1441 gcaccgaggg aatggatatc ctcgcggcct gagggagaaa gctcagaatc atgagcaccc
     1501 cacccgccgc catgccaccg gatccgcccc agtaccaagc cgccttgtgc gcctcacgcg
     1561 cagcttcatc gatcgatcgc atccacagcg taccgacgag gatcgcgccg gccatgacga
     1621 tcaccgcgaa gatgcccatc ccccacatca tagccttgtc gaggccgacc tctgccgaag
     1681 cgctcaggtg gcctaccaga tagcccaagc cgacggagcc accgacagtc actgccgcca
     1741 ggatcacgcc gccgataacg gtttgaagaa cgcgtttgcg cgtgacgaca ttcatggtgg
     1801 acaccccgat ctctatgaca agggtctttt acaaggcgtt cgagataagt caagcaccct
     1861 tgtcaccctg gcagcgctct agctatcggg ttcgtcgagc aaggccagcg cagggacctc
     1921 cgctgtgggt tcaggcaggg cgacggtctc gacgtttcgc aaactatgca ccaccgcctt
     1981 atggcgacgg gtgacgtcgg aaaccttgtt gcgggccttc tccagcgacg tgtcgacagc
     2041 ctcgagagca tcgccgtact tctcgaacgc cgccttgacc ttgccgagct cggtccagac
     2101 ctcactggat ttcttctgga tctgcagcga gcggaagccc acccgcaggc tgttctacag
     2161 ggctgtcagg gtgctgggcc ccgccaactc gaccttgtaa cgagcgcgca gatcggcagc
     2221 gaaaccttcg cgtcgaacga tctcggcaaa caagccttcg gtgggcagat agaggtaggc
     2281 gaagtcagtg gtgttcggcg gattgatgta tttctcggcc atcgaacggg cttgcgcggc
     2341 aagagcgcgt tcgagtccct tgcctgctgc ttcggcagcg accggatcgg ctgcttcctg
     2401 ggcagcgacc agccgggcat agtcctcatg ggggaatttg cagtcgatag gcagccagac
     2461 ctggccgttg tcgtcctgcc cgggaaggcg cacggcgaag tcgacgcgct cctggctccg
     2521 cggccggacc tgaacctgcg tcgcgtattg ttccgtggtg agcgtgtcct cgagcagggc
     2581 gccgagctgg acctcgcccc acgagccccg cgatttcacg ttggtcagca cgcgcttgag
     2641 gtcgccgacg cccgtggcga gcgtctgcat ctcgccgagc cctttctgca ccgactccag
     2701 ccggtccgag acctgtttga agttcgcatc cagtcgctgg tccaaggtcg attgcagctt
     2761 ttcatcgacg gtcaggcgca tctgttccag cttggcctca ttctccttac gaaggttctc
     2821 gagattgccg gacagagtgg tgcggacctt ctccaactcc tcctgcatgg ccttgcggcc
     2881 ttcaagttgc tgggcggtca gggtctcggt cagctttgcg acggctgtgt tggtctcggc
     2941 cagccgcaag cgctggctct cggcgaggtt ctcgccagcc ttctgctgaa cctcggcaaa
     3001 ggcagtgaag tggcgattga cgctttcggt caggtgggcg acgcgaccgt ccagtccgcc
     3061 ccccatcttg gccagagcgt catggagctc catccgctga gtggctgact tctggtccag
     3121 ttcgccgcgc agttctccaa gtgagcgacg cagcatttcc gcgccctttt ccggatcgtt
     3181 aagccccgcg agatgcgccg cccgggccgc agacattccg gcgaatatcg cggcgccggc
     3241 ggcaactagc gccactgcca aggtcagaag ggagagaagt tcaggggaca cagggcaggc
     3301 tcctaagtga cgacggcggc cataggcgcc gatgccgacg accaaatcgg tcgcagactc
     3361 atgcgttcgc ccttcgttcc taaagagagt cgccgttctg gagaagcggc gtcaggcggg
     3421 tcgtctggcc cgtagcgcct gcacgatcgt gccgtcatcg agccagtcga gatctccgcc
     3481 gacggggacg ccccgcgcca gagaggtcac ctccacgccg ctgcctgaca ggcgttcagc
     3541 caggtagtgg gccgtggtct gaccatcgac ggtggccggg agcgccagca cgacctcccg
     3601 cacagcgccg ccctccccgt ttgttccgcc tgtggcgttg ccggcgcgcg cgcgaccgac
     3661 cagctcagcg acacgaaggg cctctggacc gatgccatcc agcgccgaga gaagcccgcc
     3721 caacacatga tatttgcccc ggaaagcgcc ggaccgctcc atggcccaca gcgaacctgc
     3781 ctcctcgacg acgcagatca ggccattgtc gcgatgggga tcggaacaga tcgagcaggg
     3841 atcgcgcgtg tccggcgtgc cgcagaccga gcaggagacg accttctccg ccgtctcggc
     3901 catagcggcg gccagcggga tcagcagttg ctcgcggcgc ttgagcagtt gcaacgccgc
     3961 gcgtcgagcc gagcggggac ccagccccgg cagcttggcc agcagcgcga tcaaacgctc
     4021 gatttcaggt ccggcggaag cggccaatca gaacagcttc ggcatgccgg gaatgttcat
     4081 tccggccaga ggaccggcgg cctgacgcat cagctcgccg ttgatttcat ccagcttgcg
     4141 cttggcgtca gcatgagcgg cgacgatcag gtcggcgagg atttcgcctt cgccggggac
     4201 cagcaggctg tcgtcgatct tcaccgaagt gatttcgccg gggcctttga gggcaatcgt
     4261 gacgaggccg ccgccggagg ttccctcggc ggtcgtatcg gccatcttcg cctgggcgtc
     4321 ctgcagcttt tgctgcatgg cctgggcctg ctgcatcaga gagttgagat ctttcatggc
     4381 ctctagataa gacgaagcgg cgtgatgcga caggggtcgc gccgagaact gacttgcaaa
     4441 gttggcgacg ttcgatcagc gaattcccga accgccggat acgctcttgc caagcgggcc
     4501 cggaagccta tttagcaact gtacaagttg ctattcataa acaggacgcc ttccatggct
     4561 tatgacgcct ccacacgcga cgcagtgctc gacgataacg cgctggccca gttgttcacc
     4621 gaagcccgta cgcgcaatgc gttcagcgac cgccctgtcg cacccgaact gctgcacagg
     4681 ctctatgacc tgacgaaatt cgggccaaca gcggccaaca cgacgccggg acgcttcctg
     4741 ttcatcacct cgtccgaggc caaggcgcgc ctgacgccct tcatgtccga gggtaacaag
     4801 gccaagacga tgtcggctcc ggttaatgtc atcgtcgcct atgacggcga atttcatgag
     4861 cagatgccgt tcctgttccc gcataacccg gacgccaaga actggttcgg cgatgaagac
     4921 gcccgcaaaa tcccgggcgt gctgaacagt tcgcttcagg ccggctattt catgatcgcc
     4981 gcccgcgcgc tcggcctcga tgtcggcccg atgattggct tcgacgccgc cggtgtgaag
     5041 gcggagttcc tagccgatgc gacatgggtc ccgtcgttcg tgatcaacct cggctacggc
     5101 accgacgaga acctgttccc acgcttcccg cgcctgccat tcgacacggc tgcgaagatc
     5161 atctgactct gcaaaacaga aacggcggga gctgtgtccc gccgttttcg tctctagtcc
     5221 tcgtcgtcga ccggatcgtc ggggatggcg ggcatctcga tggtcggcgc cagcgtgcgg
     5281 atgtcggaaa taacggcgcc cgggaacgcc gccagcaccg aggcgacgaa ggggtcggcc
     5341 tcgacctcgg cgcgcacctg agcccggtcg ctcttttcct gctcgatcag agtctgaccg
     5401 ccgccttgac cgttggcagc gatcagccag gtgcgcccgg tccattcgcg cagccgcgag
     5461 gccagacgcc gggccagatc gatcggcgcc ccctcgacgc tctcatagac gatggcgccc
     5521 ggcttgaacg agacgggttt gacgtagcgc tgcacgtcca tctgcagact gatctcgcgc
     5581 ctctggccga tcagcgcgac ggtcgcctcg aaagtctggg gatccggcag agcgggcggg
     5641 gcagccggac gcggggcaag cgctacggat gatccgcctc ccccaccgcc gccgccacgg
     5701 gtcggtccgc ctgaaccgcc tccgagggtc tcgccgttct gaagcttctt cagcgcatct
     5761 tcgggcgcag gcaggtctgc cgcataggcc agccgcacga tgatcatctc gacggcgtcc
     5821 gccgggctcg gcgcgcgcct cacttcttca agacctttga gcagcatctg ccaggtgcgt
     5881 gacagggtgc ctgccgagat cgctgctccc agcgccgcga gcgtcttggc ctgatcggcg
     5941 ggcaaacgcg tcgcattcgg cccaagcatc ttggcgaccg aggcggcgtg gcaatgctcc
     6001 agcaggtcgt tggtcacctg gatcgggtcg gcgccgtagc cgtacagggt ccggaaggtc
     6061 tccagcgcct cgggcgtgcg accgcccatg atctgttcga acagggcgat ggtctgggac
     6121 cggtcggcca gacccagcat gtcgcgcacg acctcggtct tgaccgtctc gccgcgctcg
     6181 ccctgcacca gggcctgatc cagcaaagac agcccgtcgc ggacggagcc ctcggcagcg
     6241 cgggcgatca gggcgaggg
//
LOCUS       DASVTM010000069         7960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_166373,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000069 DASVTM010000000
VERSION     DASVTM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166373"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VF633_01820"
     CDS             <1..402
                     /locus_tag="VF633_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX8469824.1"
                     /translation="AAAVIALVAAPALAQTGEATITLTFDTAASTGGVMVALYGSEDA
                     YGHGAAITNTMVNIAAGQHTATFTVPAGDYAVKAFHDVDGDGKMNTNPFGMPTEPYAF
                     SNNARGNMGPATWSQTHFTASGVTARTISLR"
     gene            510..1976
                     /locus_tag="VF633_01825"
     CDS             510..1976
                     /locus_tag="VF633_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX8469825.1"
                     /translation="MTPTRRNFLMGAAALSAAVATPEMVRAAAALDATADWALVTRDL
                     EDDIAPRNLRLIRGRAPAGLEGTLFRNGPAKFRRPGGSATHWFDGDGLMRSFDIRDGK
                     ATLQARFADTQKRRLETRLGAVVTPGFGTAGDSRAETDSNDAANAANTSVMVAGDKVW
                     ALWEAGSPMAMDPTTLVTEGFVTLRDDLKSMPFQAHPRYAPDGTIWNVGTSGDRMIVW
                     HMRADGGLIDASIVRLPRNSYFHDFTATDGHLVIVLQPWVFEHHVMPVASQFAWQPEL
                     GTQVLVLDKADLTRTRLFELPTFFFFHLGDAWADNDGTIRFDVAAGKDIQFAIEGARV
                     LVEQRGRVPGEPSELSLITLHADGRADMVSSGLTAEFPKANPRFAGLRRDLTVHTHGE
                     TSGRPLPTGLAVHDWSRGTSDAFEFGTSHIVEETIFVPRPGARTERDAWLIGPTVNLR
                     AGVTELHVFDAAHVSDGPVATWQADVALPAGFHGAWKG"
     gene            complement(1983..2765)
                     /locus_tag="VF633_01830"
     CDS             complement(1983..2765)
                     /locus_tag="VF633_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8469826.1"
                     /translation="MSPALNSPNITPRKPATLGLFRSIVVAGAAGVVLAVAGAFGTGS
                     APLWLRLVYWMPMILAGSLWGHLCSRLIERRIDRDDQPWLTVAVLTVSITAPVSVVVW
                     MFTGLAFEHRMYPVQTLQYMIVPVGVVTMTLSAINVFLSRAQPVQTHAALVGATPARF
                     PDRLPIKLRGARIRAVQSEDHYLRIHTDRGSGLILMRFADALEELDGLEGAQTHRSWW
                     VARGAVREVARGDGRATLSLDGGLTAPVSRRYAKALRDAGWY"
     gene            2835..3482
                     /locus_tag="VF633_01835"
     CDS             2835..3482
                     /locus_tag="VF633_01835"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX8469827.1"
                     /translation="MKIILAITTLFVPALLAMSPADAVRAQQIGRPPMAPPPRNAPVV
                     PVTTAEPLAPSLSETRRPGSAALSPVTSQPATSSRPIVPPTQTRPARGTQTSLLVEVQ
                     TTQTTGQIAVAIYRDPESFRRSQNPVRTLMLERKGAVTSAFVVGLPPGRYVIAAFQDT
                     DGDGKLSKGNFGIPREPFGFSNNARARFGLPGFDAAAFELTTAGTTQRVVLRGLF"
     gene            complement(3568..4329)
                     /locus_tag="VF633_01840"
     CDS             complement(3568..4329)
                     /locus_tag="VF633_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8469828.1"
                     /translation="MTQSAPFPANDTPAKPEDAAVATPPPARRGRPPKTVGAAATDTR
                     ATILDAAEDLFSKHGFYGVTIREVAREAGVDTALVHYYFGAKRELFDAVFLRRAEVWN
                     NDRVDAINRYVETNGASATLEGLLEAFLRPPFEWSLKGGPGWKHYAALVAQTNANPTF
                     GGETMARYFDPAIHRLIELIREMIPGSKDVDLYWAYHNLSGALTLTLGETGRLDRLSG
                     GLARSGDLESACDYMVRFAAAGIRAVCGPTLPPQT"
     gene            4509..6716
                     /locus_tag="VF633_01845"
     CDS             4509..6716
                     /locus_tag="VF633_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469829.1"
                     /translation="MNRLVKSALLAGAAWSAISTGAMAQDAIPQEEAATVGDVVVTAR
                     RRDEQLKDVPVAVSALSAERLEQSGAVDITALQQQTPNATVQIARGSNSTLISFIRGV
                     GQQDPLWGFEPGVGLYIDDVYVARPQGAVLDIFDIQRIEVLRGPQGTLYGRNTIGGAI
                     KYVTKKLSDDPTFLGRATFGAYNEHNFLLSGSVPIGDTLAIGGAVATYNHDGYGKNLN
                     TGMDQYDKDVTAYRASAEWTPSNQLFFRLAYDRVEDNSNPRHGHREVNSTVGGYTPPA
                     SVYDTNAGITGEQKVVTDGVSLTGEYQINDMVTFKSITAYRSGDTATVIDFDETPLPT
                     LDIPAVYSDDQFTQELQLLFTGDRWSGVAGLFYLDGTSSGAFDTIAGNLGLSIAAAGS
                     VETKSYSAFADFSYDITDRLHASLGGRYTKDDKDATVFRAFYLGATNSPGLGGTPRPI
                     FATRTNYAASDSFEKFTPRASVSYDFSDDITGYASVGQGFKSGGWDMRGDAALVPQTV
                     NGYKPETVTTYELGLKGTALDHRLSFASAAFYSDYTDQQITTQQVATAPAVGIASVVD
                     NAGASTIYGFELEGSAFLSDDITANFSVGYLHNEFDQFITLVTGTPVDISNIREPQNS
                     PEWSAYYGMTFRGDILGGVIRVTPSLSYRSSYHLFDTPDPILDQKGYVLADAAVTWAA
                     PNGHYEIGLFGRNLTDQEYRVGAYSFPGATYNNSISAFYGPPRTYSVQLTVKY"
     gene            6887..>7960
                     /locus_tag="VF633_01850"
     CDS             6887..>7960
                     /locus_tag="VF633_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8469830.1"
                     /translation="MATNEAAAPQARVERPGYRYYVLAILILVYMLNFLDRQIIGILA
                     APLKAEFGLTDTQFGLLGGIAFASVYSTLAIPLAALADKTSRVWIMTGALAVWSSFTA
                     LCGVAGSFGQLFLFRMGVGVGEAGGVAPAYSLISDYFPPNQRARALAGFAFGIPLGTA
                     AGTLVGGLVAASYGWRTAFIVVGLLGLLIAPLLRLTVRDPVRGGTDAVRAPLTAVESA
                     APPVVVSEKVGRTASLILLGLGAGCLILAALNHFAGLAVGNALVLAFGGLLAVVIGVS
                     FWIARRTASVVMPKPSFWLLALGAASSSVCGYGIAGWLPLFFMRSFDLTLAQTSWYYS
                     GIALIGGLLGIWLGGSIAAKLAKR"
BASE COUNT         1345 a         2791 c         2465 g         1359 t
ORIGIN      
        1 gccgccgccg tcatcgccct ggtcgccgcc ccggcattgg cccagacagg tgaagccacc
       61 atcactctga ccttcgacac cgcagcctcg acgggcggcg tcatggtcgc gctgtacggc
      121 agcgaagacg cctacggtca cggcgctgcg ataaccaata cgatggtcaa catcgcggcc
      181 ggtcagcata cggccacctt cacggttccc gcaggcgact atgcagtgaa ggccttccat
      241 gacgtggatg gcgacggcaa gatgaacacg aacccctttg gcatgccgac cgagccctac
      301 gcgttctcaa acaacgcgcg cggcaacatg ggtcccgcga cctggagcca gacgcatttc
      361 accgcctccg gcgtgaccgc ccggacgatc agcctgcgct gacatccttt ccgggggcta
      421 acgcgcttcg cgctacttga gcccgcagac ccccggaaac catccggccc ttgcctcttc
      481 tcctctcctt cctgctcgtg agctccgcca tgacccccac ccgtcgcaac ttcctcatgg
      541 gcgcagccgc cctgtccgcc gccgtcgcaa cacccgagat ggttcgcgcc gccgccgccc
      601 tcgacgcgac cgcagactgg gccctggtca cccgtgatct ggaagacgac atcgcacccc
      661 ggaatctacg tctgatccgg ggccgggcgc cggcggggct ggaggggacg ctgttcagga
      721 atggcccggc caagttccgg cgtccgggcg gctcggcgac ccactggttc gatggcgacg
      781 gactgatgcg gtccttcgac atccgcgacg gcaaggcgac gctgcaggcc cggttcgccg
      841 acacgcagaa acgccgcctc gagacccggc tcggcgccgt cgtcaccccc ggcttcggta
      901 ccgccggcga cagccgggcg gagaccgact ccaacgacgc cgccaacgcc gccaacactt
      961 cggtgatggt cgccggcgac aaggtctggg ctctctggga ggccggttcg cccatggcca
     1021 tggatccgac gaccctcgtt acggaaggct tcgtcaccct gcgcgacgac ctcaagagca
     1081 tgcccttcca ggcccatccc cgctacgccc ccgatggcac gatctggaat gtggggacca
     1141 gcggcgaccg gatgatcgtc tggcatatgc gcgccgacgg aggactgatc gacgcgtcca
     1201 tcgttcgcct gccgcgcaac agctacttcc acgacttcac cgccaccgac gggcatctgg
     1261 tgatcgtgct gcagccttgg gtgttcgagc atcatgtcat gccggtcgcc agtcagttcg
     1321 catggcagcc ggaactcggc acgcaggttc tggtgctgga caaggcggac ctgacgagga
     1381 cgcgtctgtt cgaactgccg accttcttct tcttccacct tggcgacgcc tgggccgaca
     1441 acgacggcac gatccgcttc gatgtggctg caggcaagga catccagttt gccatcgagg
     1501 gcgcgcgggt gctggtcgag cagagaggcc gggtgcccgg agaaccctcc gagctctcgc
     1561 tcatcaccct gcacgccgat ggtcgcgccg atatggtgtc cagcggcctg actgccgaat
     1621 tccccaaggc aaacccacgc ttcgccggcc tgcgccgcga cctgaccgtc cacacccatg
     1681 gcgagacatc gggtcgtccg ctgccaaccg gactggcggt tcacgactgg tcgcggggga
     1741 cctcggacgc cttcgaattc ggtacgagcc atatcgtcga ggaaacgatc ttcgtgccac
     1801 gaccgggcgc ccgcacagag cgcgacgcct ggctgatcgg cccgacggtc aatctgcgcg
     1861 ctggtgtgac cgagcttcac gtgttcgacg ccgcccacgt ctcggacggt cccgtggcga
     1921 cctggcaggc cgacgtggcc ctgcccgcag gctttcacgg ggcgtggaaa ggttaagccg
     1981 gttcagtacc agcctgcgtc tcgcagggcc ttggcatagc ggcggctaac gggcgccgtc
     2041 aggccgccgt ccagcgacag ggtcgcgcga ccatcgccgc gagcgacctc gcgcacggcg
     2101 ccacgggcca cccaccagct gcgatgggtt tgcgcgccct cgagcccgtc cagctcctcc
     2161 agggcatcgg cgaaccgcat caggataagg cccgagcccc ggtcggtatg gatacgcaga
     2221 tagtggtctt cggactgaac cgcgcggatc ctcgcgcccc tcagcttgat cggcagccgg
     2281 tccggaaagc gagccggagt ggcgccgacg agggccgcat gggtctggac cggctgggcc
     2341 ctggagagga agacgttgat ggcagacaac gtcatcgtca cgacgcccac gggcacgatc
     2401 atgtactgca gggtctggac cggatacatc cggtgttcaa aagccagacc ggtgaacatc
     2461 cagacgacca ccgagaccgg ggcggtgatg gacacggtca gaaccgcgac cgtcagccat
     2521 ggctggtcgt cacgatcgat ccgccgctcg atcagacgtg agcacaaatg tccccacagc
     2581 gatcccgcca ggatcatcgg catccagtaa accagcctga gccacaaggg cgcggacccg
     2641 gtgccgaacg cccctgcgac ggccagaacg acgcccgcgg cgccggcgac gacgatgctg
     2701 cggaaaagac ccagcgtcgc aggcttgcgg ggcgtaatgt tgggggaatt cagggcggga
     2761 ctcattgcat tctgtggcgc ttcgccgtca tggttggcgg cggtaacgct gtgagaccac
     2821 gaaacggccc cgaaatgaag atcatcctcg cgatcacgac actgttcgtc cccgccctcc
     2881 tggcgatgtc acccgccgac gccgtgcgcg cgcagcagat cggcaggccg cccatggccc
     2941 ctccgccccg caacgccccg gtggttcctg tgacgaccgc ggagcccctc gcgccatctc
     3001 tgtccgaaac gcgccgccct ggatcggcgg ccttgtcccc ggttacatcg cagccagcga
     3061 cttcatcgcg cccgatcgtg ccgccgaccc agacccgtcc tgcgcgggga acccagactt
     3121 ccctgctcgt cgaggtgcag accacccaga cgaccggtca gattgccgtc gctatctatc
     3181 gtgaccctga atccttccgc cgcagccaga atccggttcg caccttgatg ctggagcgga
     3241 agggcgccgt gacgtccgcc ttcgtcgttg gtctgccgcc cgggcgctac gtgatcgccg
     3301 ccttccagga caccgacggt gacggcaagc tcagcaaagg caatttcggt attccccgcg
     3361 aaccgttcgg tttctcgaac aatgctcgtg cccgctttgg cctgcccggc tttgacgcgg
     3421 cggcgttcga gttgacgacg gcaggcacga cgcagcgggt cgttctgcgc ggtctgttct
     3481 gatctgcgcg cctgaagtgg ggccgcccgt cacatgatgc agtaaggatc ggcacgtttg
     3541 ggccccgacg ggcgggcgtc ggttgcccta ggtctgcggc ggcagcgttg gcccgcacac
     3601 agcccggatc cccgccgctg cgaacctgac catatagtca caagcgcttt ccagatcgcc
     3661 agagcgcgcc agcccgccgg acaacctgtc gagacgcccg gtctcgccca gcgtcagggt
     3721 caacgcgccc gacaggttgt gataggccca gtacagatcg acgtctttcg atcccgggat
     3781 catctcccgg atcagctcga tcagacggtg gatggccggg tcgaaatagc gggccatggt
     3841 ctcgccgccg aaggtcgggt tggcgttggt ctgggccacg agcgcggcat agtgtttcca
     3901 gccgggaccg cccttgagcg accattcgaa cggcggacgc aggaaagcct cgagcaagcc
     3961 ctcgagggtc gctgaggcgc cgttggtttc gacatagcgg ttgatggcgt cgacgcggtc
     4021 gttgttccag acctcggccc gacgcaggaa gacggcgtcg aacaattcgc gcttagcccc
     4081 gaaataataa tgcaccagcg cggtatcgac gcccgcctcg cgcgccacct cgcggatggt
     4141 gacgccgtaa aagccgtgct tggaaaacag gtcttcggcc gcgtcgagga tggtggcgcg
     4201 ggtgtcagtc gccgctgccc cgaccgtttt gggcgggcgt ccgcgccggg ctggaggcgg
     4261 cgttgcgacg gcggcgtctt caggctttgc cggagtgtca ttggccggaa acggggcgga
     4321 ctgggtcatg ccgtcacgct aacccccgaa acggcgccgg tgcaacggcg ccacaggctg
     4381 acgctacgtc attgccgcaa agcgctgccg tttgacacaa gcctgaaacg cggtcaggtt
     4441 caggaaaatc attgaatgct gaataacagc gtcgatgggc tggaaacaac aggaacggga
     4501 cgaccccaat gaacagactc gtcaagagcg cgctgctggc cggtgcggcg tggagtgcga
     4561 tttccaccgg ggccatggct caggatgcga tccctcaaga agaagcggcc accgtcggcg
     4621 acgtcgtcgt caccgcccgc cgccgtgatg agcagttgaa ggacgtgccg gtcgcggtct
     4681 cggccctcag cgccgagcgt ctggaacagt ccggcgccgt cgacatcacc gccctgcagc
     4741 agcagacccc gaacgccacg gtccagatcg cgcgcggctc caactcgacc ctgatcagct
     4801 tcatccgcgg cgtcggccag caggatccgc tctggggctt cgagcccggc gtcggcctct
     4861 atatcgatga cgtctatgtc gcccgtccgc agggcgccgt gctcgacatc ttcgacatcc
     4921 agcgcatcga agtcctgcgc ggcccgcaag gcacgctgta cggtcgcaac accatcggcg
     4981 gcgccatcaa atatgtgacg aagaagctga gcgacgaccc gaccttcctg ggacgcgcga
     5041 ccttcggagc ttacaacgag cataacttcc tgctctcggg ctcggtgccg atcggcgaca
     5101 ccctggccat cggcggcgcg gtcgccacct acaaccacga cggttacggc aaaaacctca
     5161 acaccggcat ggaccagtac gacaaggacg tgaccgcgta tcgtgccagc gccgaatgga
     5221 cgcccagcaa tcagctgttc ttccgcctcg cctatgaccg ggtcgaagat aactccaacc
     5281 cgcgccacgg ccaccgcgag gtcaactcga ccgtcggtgg ctatacgcct ccggcaagcg
     5341 tctacgacac caacgccggc atcaccggcg agcagaaggt ggtcacggac ggcgtctcgc
     5401 tgactggcga ataccagatc aatgacatgg tgacgttcaa gtcgatcacc gcctatcgtt
     5461 ccggcgacac ggcgaccgtc atcgacttcg acgaaacgcc gctgccgacg ctcgacattc
     5521 cggccgtcta ttccgacgat cagttcaccc aggaactgca gctgctgttc accggcgatc
     5581 gctggtctgg tgtcgcgggc ctgttctatc tggacggcac ctcgtcgggc gcgttcgaca
     5641 ccatcgccgg aaacctcggg ctgtccatcg ccgccgcagg ctcggtcgag acgaagtcgt
     5701 actcggcctt cgccgacttc agctacgaca tcaccgaccg cctgcacgcc tcgctcggcg
     5761 gccgctatac caaggacgac aaggacgcga ccgtgttccg cgccttctac ctcggcgcca
     5821 ccaacagccc cggactgggc ggtacgccgc gtccgatctt cgccacccgc accaactatg
     5881 cggcctcgga ctcgttcgaa aaattcacgc cccgcgccag cgtcagctac gacttctccg
     5941 acgacatcac gggctacgcc tcggtcggtc agggcttcaa gtcgggcggc tgggacatgc
     6001 gcggcgatgc agctctcgtt ccccagacgg tcaatggcta caagcccgag acggtcacga
     6061 cctatgagct gggccttaag ggcaccgcgc tggaccaccg cctgtccttc gcctccgccg
     6121 ccttctattc cgactacacg gaccagcaga tcacgaccca acaggtcgcg accgccccgg
     6181 ccgtcggcat cgcctcggtc gtcgacaacg ccggcgcctc gaccatctac ggcttcgaac
     6241 tcgaaggctc cgccttcctg agcgacgaca tcacggcgaa cttctcggtc ggttacctgc
     6301 acaatgagtt cgaccagttc atcaccctgg tgacgggcac gccggtcgat atctcgaaca
     6361 tccgcgagcc gcagaactcg ccggagtggt cggcctatta cgggatgacg ttccggggcg
     6421 acatcctcgg cggggtgatc agggtcacgc catcgctgtc gtaccgctcg agctaccatc
     6481 tgttcgatac gcccgatccg atcctcgacc agaagggcta cgtgctggcc gacgccgccg
     6541 tcacgtgggc tgcgccgaac ggccattacg agatcggcct gttcggtcgc aatcttactg
     6601 accaggagta ccgtgtcggc gcctacagct tccccggcgc gacctacaac aactcgatca
     6661 gcgccttcta cggcccgccg cgcacctatt cggtgcagct gaccgtcaaa tactgatcgg
     6721 ctagacgtcg aacggacaag ggcggcggtt ctccggcaac gggatccccg ccctttttcg
     6781 tttgggcctt ttcgttccgg gcctttttcg tttgggcctt ttttcgttgc ggtccccagc
     6841 ttgaccgcta ccataccgaa aaccagaaaa accccgagga agcgacatgg cgacgaacga
     6901 ggcggccgcg ccgcaagccc gcgtggaacg gcccggttac cggtattacg tgctggcgat
     6961 cctgatcctc gtctacatgc tgaactttct ggaccgccag atcatcggca ttctcgccgc
     7021 tccgctgaag gccgagttcg gcctgaccga tacgcagttc ggactgctgg gcggcatcgc
     7081 tttcgcttcg gtctactcca ccctcgccat tccgctggcg gcgcttgccg acaagaccag
     7141 ccgcgtgtgg ataatgaccg gggcgctcgc cgtgtggtcg agctttaccg ccctgtgcgg
     7201 cgtcgccggt tcgttcggcc agttgttcct gttccgaatg ggcgtgggcg tgggcgaggc
     7261 gggcggcgtg gcccccgcct attcgctgat ctccgactat ttcccgccca accaacgcgc
     7321 acgcgcgctg gccgggttcg ccttcggcat cccgctcggc accgccgccg gcaccttggt
     7381 tggcggcctc gtggctgcga gctatggctg gcgcaccgcc ttcatcgtcg tcgggcttct
     7441 gggcctgctg atcgcgccat tgctgcgcct gaccgtcagg gatcccgtac gcggcgggac
     7501 cgacgccgtc cgcgcgccgc tgaccgccgt cgaaagcgca gccccgccgg tcgtggtctc
     7561 cgaaaaggta ggccgcacgg cttcgctgat cctgctcggc cttggcgcgg gctgcctgat
     7621 cctcgccgca ctgaaccatt tcgcaggcct cgcggtcggc aatgcgctgg tgctggcgtt
     7681 cggcggcctg ttggccgtcg tcatcggcgt gtccttctgg atcgcccgca ggaccgcgtc
     7741 cgtcgtcatg cccaagccga gcttctggct gctggcgctg ggagccgcgt cctcgtcagt
     7801 ctgcggttac ggcatcgcag gctggctgcc gctgttcttc atgcgcagct tcgacctgac
     7861 gctcgcccag accagctggt actattccgg catcgccctg atcggcggtc tgctgggcat
     7921 ctggctcggc ggttcgatcg ccgccaagct ggccaagcgc
//
LOCUS       DASVTM010000070         9027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_432674,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000070 DASVTM010000000
VERSION     DASVTM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432674"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VF633_01855"
     CDS             <1..900
                     /locus_tag="VF633_01855"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX8469831.1"
                     /translation="VPLDYHKEGLDAEVLAHFGITDAPAPKLEMWEEIARRRLHPDGE
                     VTVAIVGKYTVLKDAYKSLIEALQHGGVANNVKVNIDWVEADTFEGDRDACEERLQTA
                     HAVLVPGGFGERGAEGKIEAARFARERNIPYFGICFGMQMAVIEAARNQAGFAKASST
                     EFGPTAEPVVGLMTEWTQGNQRVMRQQGGDLGGTMRLGAYDSTLAEGSRIAEIYGTTA
                     ISERHRHRYEVNINYREAIEAKGLVFAGLSPDGILPETVERTDHPWFIGVQYHPELKS
                     RPFKPHPLFASFIGAAVIQSRLV"
     gene            1216..2625
                     /locus_tag="VF633_01860"
     CDS             1216..2625
                     /locus_tag="VF633_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HEX8469832.1"
                     /translation="MNQRTLSRRSTLALMAAAAASPAFAGSAGQGVPSEDWPALAADV
                     KAEFKWAWDHYVDKAWGKDEINPVSGTSQSFFIEGHDLGLSIVEALDTLWIMELDDEF
                     QAGVDWVKANLDFDIDGEAQVFETNIRLVGGLLSAYLACGDPVLLAKAKDLADRLMKA
                     FDASPHGLPYRYVNLKSGAVRDPETNLAEIGTYATEFGLLGQLTGEDRYYAAAKRAMK
                     HALDMRSPLGLMAANIHAETGQFTSRNASIDVYADSFYEYLWDAWEMFGDVEMKLAAE
                     ECMAAMIAHQGHRYDGKLWFPMVDFETGVVTNTSQTVLGAYLAGLFGQVGQKAAGDDF
                     LATYTAMQEKYVIIPESTDVTTGLARSKRTGLRPEYVDACLNLWLGDRDERYRALAAV
                     HYRNMKTTSKAAFGYTALADITTRPMTQGDNCPGYWWSEQMKYYFLLFGDARRVDYGR
                     LQLSTEANVLRGFKAAGAV"
     gene            2800..3183
                     /locus_tag="VF633_01865"
     CDS             2800..3183
                     /locus_tag="VF633_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX8469833.1"
                     /translation="MPDGLSYTRAKVFRRQMTPPEARLWVRLRGSRLGGFKFRRQHPI
                     GPYIVDFYCAWVKLAVEVDGGSHDTEEQIAHDKRRTAWLGRQGVRVIRLRASSVRDEI
                     EGVMDFILASVRDRVPDGYHGVAPT"
     gene            complement(3282..5651)
                     /locus_tag="VF633_01870"
     CDS             complement(3282..5651)
                     /locus_tag="VF633_01870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEX8469834.1"
                     /translation="MVRRMVAAVLSAALAIGPASVVVAQTAAPSPYDAVDPFIGTAGQ
                     GHTFPGAVAPFGMVQLSPDTEHKVLRQGYEWASGYRYDDPTILGFSHTHFSGSGHSDL
                     GDVLLTPLAAGEIKLEPGEADTPGSGYRSRYDKASEVAEPGYYAVNLTDSGVHVELTA
                     GTRVGMHRYAFPTGAPARVLLDFRPSIYDYPGKVSWSRIRVAPDGTVTGYRETRGWAP
                     GRQLYFAMRFSRPMTSHSIQNREENVPYNGFTKPGRTPEDRAQVEGRALEAVFDFGPI
                     DGPLVVKVALSGVDEAGAIANLNSEGGDFDFNGLRASTKTAWEQALGAVEIDAPADMR
                     TTLYTALYHTLMAPSVMGDVDGRYRGPDQAVHKAEGFTFHSTFSLWDTFRAEHPLLTL
                     VQPEKRNADMINSLIASQQVSPYGLLPVWQFAGQETWTMIGYHAVPVIADAWMKGIRG
                     FDGEAALKAMVASATYRPYGGLGDYMDLGYVPIDREPEAASKTVEYAYDDWTIARMAR
                     NLGHADIAATFEKRAQNWRNSFDLETGFLRARKADGAYRTPFDPTAINYGSDYTEGNA
                     WQYSWFAPQDLGGIVGAMGGDTATVAKLDAMFDFDNSKLDYSHAEDIAGLIGQYIHGN
                     EPSHHSAYMYVYAGAPWRTQERLKQIVESQYHARPDGLAGNDDLGQMSAWLVFTGLGF
                     YPVTPGSNQYVIGRPFVDRAVLNLPNGRRFAVVAEGLSDANTYIASVTLNGRPLDRSW
                     IGDEEIRKGGELRFVMGAEPNEAWGAGAAARPYSMTGFTGQTASPSPGR"
     gene            6170..6949
                     /locus_tag="VF633_01875"
     CDS             6170..6949
                     /locus_tag="VF633_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX8469835.1"
                     /translation="MIGGLLGPRDPATVSVTNPAGRSAFLLIGDHAGRAIPERLGDLG
                     LSESDRSRHIAWDIGVRALGEALAARLDAVFISQTWSRLVIDCNRDPDRADAIPEISD
                     GSRIPGNQGLTPQERAVRIAEVHAPYQDAIASEMADRRRMSKPTVLVALHSFTPSMGG
                     FDRPWDAGVLHDGGDLRFARAMLDVMQATPGLNIGDNAPYQMDAIDHTVPRHAIAAGL
                     PYVELEIRQDHLSNDIGVNRWADWIAGWLTQSAAVMENASP"
     gene            complement(6992..7411)
                     /locus_tag="VF633_01880"
     CDS             complement(6992..7411)
                     /locus_tag="VF633_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX8469836.1"
                     /translation="MARPLERVAYVSTATLSLESLLVITDILAVSQRNNARDHLTGAL
                     EFSEGKFFQVIEGQTADVDRLMNRVMADRRHTDIEIVTRLHVEGRLFPDWSMTAPRIA
                     PEMEPAIRKAVAESRSSPTAAIEMMRRIAMEDGIHSG"
     gene            7760..8602
                     /locus_tag="VF633_01885"
     CDS             7760..8602
                     /locus_tag="VF633_01885"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX8469837.1"
                     /translation="MGYGARGFVYVSLGLLTLLAALDLGGRAVGTHGAILWLAEQPFG
                     RGWLIALGAGLWAFVAWRVLQSVFDADREGRTPRALLLRVGQAVSGLFYAVLGSGVFE
                     LLDEVGSRMGAEDVAENQRKAALLLDLPFGDALLISVGLVITAVGVGNIVKGIRSDFA
                     DSLACSERLCRRLTPVARAGYIARGAAYLPLALLVVLAGWHARASEVESFGSSLDALE
                     AQPGGSLMLGATAVGLMAFGVFAFVEARFRRIRAPRDLNPLQAGSSPFPRWQSLLAQR
                     LRKP"
BASE COUNT         1543 a         2761 c         3048 g         1675 t
ORIGIN      
        1 gtgccgctcg actatcacaa ggaaggcctc gacgccgagg tgcttgccca tttcggcatc
       61 accgatgcgc cggctccgaa gctggagatg tgggaagaga tcgcgcgccg ccgcctgcat
      121 ccagacggcg aggtcaccgt cgccatcgtc ggcaaataca cggtcctgaa ggacgcctac
      181 aaatccctga tcgaggccct ccagcacggc ggtgtggcca acaacgtcaa ggtcaacatc
      241 gactgggtcg aggccgacac cttcgagggc gatcgtgatg cctgcgagga acgcctgcag
      301 accgcccatg cggtgttggt gcccggcggc ttcggcgagc gtggcgccga aggcaagatc
      361 gaggcggctc gtttcgcccg cgagcgcaac atcccctatt tcggcatctg ctttggcatg
      421 cagatggcgg tcatcgaggc ggcgcggaac caggcaggtt tcgccaaggc gtcgtccacc
      481 gagttcggcc cgaccgcaga acccgtcgtc gggctaatga ccgaatggac gcagggcaat
      541 cagcgggtga tgcgccagca gggtggagat ctgggcggca caatgcgtct gggcgcctat
      601 gactcgacgt tggctgaggg gtcgcggatc gccgagattt acggcacgac cgccatcagc
      661 gagcggcacc gccaccgcta tgaggtcaac atcaactacc gcgaggccat cgaggccaag
      721 ggtctggtgt tcgcgggcct gtcgcccgac ggcatcctgc ccgagacggt cgagcgcact
      781 gaccacccgt ggttcatcgg cgttcagtat caccccgagc tcaagagccg accgttcaag
      841 ccgcatccgc tgttcgccag cttcatcggc gcggcagtga ttcagagccg gctggtctag
      901 ggcctcatgg gaccgggacg ggtgggcttg ccagaagctt gtggaacgtg aaaacttgaa
      961 cacggttctt gaacggtttc atgttatttg catgttgcgt tccagcggtg aggcagaacg
     1021 cagggtccgt gggaggacac agcaatggtt ggtgagttca tcacctttct ccaccggaac
     1081 atccgggtcg tgacgcccgc cgtgttgctg gtgggagccg gggccgtgtt cgcgctcacg
     1141 catctgctgc acgcccggta gctgtaccgt tattaccggc tggaaagctc aaccggtttg
     1201 tccgaacata cgggcatgaa ccagcgcact ctgtctcgac gttcgaccct cgccctgatg
     1261 gccgccgcag ctgcatctcc ggcatttgct gggtcggcag ggcagggcgt tccgtctgaa
     1321 gactggcctg cgctcgccgc agacgtgaag gccgagttca aatgggcctg ggaccattat
     1381 gtcgacaagg cctggggcaa ggacgaaata aatccggtct cgggcacgtc gcagtcgttc
     1441 ttcatcgagg gccatgacct cggcctgtcc atcgtcgagg cgctcgacac cctgtggatc
     1501 atggagttgg acgacgagtt ccaggccggg gtcgactggg tcaaggccaa cctcgatttc
     1561 gacatcgacg gcgaggccca ggtgttcgag accaatatcc ggttggtcgg cggattgctg
     1621 tcagcctatc tggcctgtgg cgacccggtg ctgctggcca aggccaagga tctggctgac
     1681 aggctgatga aggcgttcga cgcctcgccg catggcctgc cgtaccgcta tgtgaacctg
     1741 aagagcgggg cggtgcgcga ccccgagacc aatctggccg agattggcac ctatgcgacc
     1801 gagttcggcc tgttaggtca actgaccggg gaggaccgct actatgccgc agcaaaacgg
     1861 gcgatgaagc atgcgctcga tatgcgctca ccgctgggtc tgatggcggc gaatatccac
     1921 gccgagacgg gccagttcac cagccgcaac gcctcgatcg acgtctatgc cgacagcttc
     1981 tacgaatacc tctgggacgc ttgggagatg ttcggcgacg tcgagatgaa gctcgccgcc
     2041 gaggaatgca tggccgccat gatcgcgcat cagggccacc gctacgacgg caagctgtgg
     2101 ttcccgatgg tcgatttcga gacgggcgtt gtgaccaaca cctcacagac ggtgctgggc
     2161 gcctatctgg ccgggttgtt cgggcaggtg ggccagaagg ccgccggcga cgacttcctc
     2221 gccacctaca cggcgatgca ggagaaatac gtcatcatcc cggagtccac ggatgtcaca
     2281 acgggtctgg cgcggtcgaa gcggacgggg ctgaggccgg aatatgtcga cgcgtgtctg
     2341 aacctttggc tgggggaccg agacgagcgg taccgggcgc tggcggcggt tcactatcgc
     2401 aacatgaaga cgacgtcgaa ggccgcgttt gggtatacgg cgctggccga catcacgacg
     2461 cgtccgatga cgcagggtga caactgcccg ggttactggt ggtcggaaca gatgaagtat
     2521 tacttcctgc tgttcggcga tgccaggcgg gtggactacg ggcggctgca gctgagcacg
     2581 gaagcgaatg tgctcagggg gttcaaggcg gccggcgcgg tgtagctctc tcctgaccgc
     2641 tgcgcggtct cacctctcca ttcgccttcg cgaatggaga ggtgagttgc ggcgttcacc
     2701 tctccatcgc cgaagtgcga tggagaggac agatcggcgt cttcgtcgat caggtgaggg
     2761 cgactcgacg cctgaagtta tacgcgccat agttgtttca tacccgacgg cctcagctat
     2821 acgcgtgcga aagtcttccg ccgtcagatg acgccgccgg aggcgcggct gtgggttcgg
     2881 ttgcgcgggt cacggctggg cggtttcaag tttcgccgcc agcatccgat cgggccgtac
     2941 atcgtcgact tctactgcgc ttgggtgaag ctggccgtcg aggtcgatgg cgggtcgcac
     3001 gacaccgagg agcagatcgc gcacgataag aggaggacgg cgtggctcgg gagacagggc
     3061 gttcgcgtga tccggctgcg ggcgagctcg gttcgcgacg agattgaggg cgtgatggat
     3121 tttatactgg cgtccgtgag ggaccgagtc ccggacggct accacggtgt cgcccccacc
     3181 tgactgctgc gcagtctgtc ctcccatcgc ccttcggcga tggggaggtg agtagcatca
     3241 cctctccatt cgggaaggcg aatggagagg acaggtcgct cttagcgacc aggagagggc
     3301 gacgcggtct gtccggtaaa gcccgtcatc gaatacggcc tcgccgccgc ccccgcaccc
     3361 caggcctcgt tcggctcagc ccccatgacg aatcgcagct cgccgccctt gcggatttcc
     3421 tcgtcgccga tccagctgcg gtccagcggg cgaccgttca acgtcaccga cgcgatgtag
     3481 gtgtttgcgt ccgacagccc ctcggccacc accgcgaacc tccgcccgtt cggcaggttc
     3541 agcaccgcac ggtccacgaa cggccgaccg atcacatact ggttgctgcc cggcgtgacc
     3601 gggtagaagc ccaatccggt gaacaccagc caggccgaca tctggccgag gtcgtcgttg
     3661 ccggcgaggc cgtcggggcg ggcgtggtac tggctttcga cgatctgctt cagccgctcc
     3721 tgtgtccgcc atggcgcgcc ggcatagacg tacatgtatg ccgagtggtg gctgggttcg
     3781 ttgccgtgga tgtactggcc gatcaggccg gcgatgtcct cggcgtggga atagtcgagc
     3841 ttcgagttgt cgaaatcgaa catggcgtcg agcttggcga cggtcgcggt gtccccgccc
     3901 atggccccga cgatgccgcc cagatcctga ggcgcgaacc acgaatattg ccaggcgttt
     3961 ccctcggtgt agtcggatcc gtagttgatg gcggtggggt cgaagggcgt ccggtacgcg
     4021 ccgtcggcct tgcgcgcgcg caggaagccg gtctcgagat caaagctgtt cctccagttc
     4081 tgggcccgct tctcgaacgt cgcggcgatg tcggcgtggc cgaggttgcg ggccatgcgg
     4141 gcgatggtcc agtcgtcata ggcgtactcg actgtctttg acgccgcctc cggctcccgg
     4201 tcgatgggga cgtagccgag gtccatatag tcgccgagcc cgccgtaggg tcggtaggtg
     4261 gcgctggcca ccatggcctt cagcgccgcc tcgccgtcga agccgcgaat gcccttcatc
     4321 caggcgtcgg cgatgaccgg gacggcgtga tagccgatca tggtccaggt ttcctgaccc
     4381 gcgaactgcc agacaggcag caggccgtat gggctgacct gttgcgaggc gatcagggag
     4441 ttgatcatgt cggcgttgcg cttctccggc tggaccagcg tgagcagtgg atgctcggcg
     4501 cggaaggtgt cccacagcga aaacgtcgag tggaaggtga agccctcagc cttatgaacc
     4561 gcctgatcgg ggccgcgata ccgaccgtcg acgtcgccca tgacgctggg cgccatcagg
     4621 gtgtgataga gcgcggtgta cagggtggtg cgcatgtcag ccggagcgtc gatctcgacc
     4681 gcgcccagcg cctgttccca tgccgtcttc gtcgaggcgc gcaagccatt gaaatcgaag
     4741 tcgccgcctt cgctgttcag gttggcgatg gctccggctt catcgacgcc ggacaaggcg
     4801 accttgacca ccagcgggcc gtcgatgggg ccgaagtcga atacggcttc cagcgcgcga
     4861 ccttcgacct gggcgcgatc ctcgggcgtg cggcccggct tggtgaagcc gttgtagggg
     4921 acgttctcct cgcggttctg gatcgagtgg ctcgtcatcg gcctggagaa ccgcatggcg
     4981 aaatagagtt gacggcccgg cgcccagccg cgcgtctcgc gatagccggt gaccgtgccg
     5041 tcgggcgcga cacggatccg cgaccacgac accttgcccg gatagtcata gatcgagggc
     5101 ctgaaatcga gcagcacccg ggccggagcg ccggtcggga aggcatagcg gtgcatgccg
     5161 acgcgcgtgc cggccgtcag ctcgacgtgg acgccggagt cggtcaggtt caccgcgtag
     5221 tagccgggct cggcgacttc ggaggccttg tcgtagcgtg agcggtaacc ggaccccggc
     5281 gtatctgctt cgccgggctc cagcttgatc tcgcccgccg ccaacggcgt cagcaggacg
     5341 tcgccgaggt cggagtggcc cgagcctgag aagtgggtgt gggagaagcc gaggatggtc
     5401 gggtcatcgt agcgataccc tgaggcccat tcgtagccct gacgcagaac cttgtgctcg
     5461 gtgtcagggc tgagctgcac catgccgaag ggagccacgg cgcccgggaa ggtgtggccc
     5521 tgacctgctg tgccgatgaa cggatcgacg gcgtcatagg gggagggggc cgccgtctgg
     5581 gccaccacga cactcgccgg gccaatcgcg agcgccgcgc tcaggactgc cgccaccatc
     5641 cgacgcacca tgacgactct cctacaccgc acagggactt cacatgatca ggacagcgac
     5701 ctcaagcgcc gaggttcctc accccagggt ttcgcgtctg cggcgtatgc gcttggccaa
     5761 gcttcgccat tggctctgta tgcagtggac tatcggatgc gaccgcgtct gaaggacagg
     5821 ggagctcata tgccaaagga aatcatcgcc ggagccgtcg gtatggggct cgtcagcatc
     5881 atgtgggtca ttcaacggct gatcgctttt tccgcgtccc ggaaagaacg cctgcgtttc
     5941 taggacctcg cgctgaggac acaggctttt ccactaggtg aaccggtgtt ttgtgtggtt
     6001 catggaagac ggccaggggg gcaagcctcg gtcaggcggc ccggtggatt tccagtcacc
     6061 aagccgggcc gcttccattc cttttcgaaa atccgcatcc cttcggggac aggcgcgcta
     6121 gatattccgg tagcggtcta accggctgtc aggcgaaaag cgtgagacga tgatcggcgg
     6181 acttctgggg ccaagggatc cggcgacggt atcggtgacc aatccggccg ggcggtcagc
     6241 tttcctgctg atcggggacc atgcaggccg ggcgatcccg gagcggctgg gtgatctggg
     6301 cttgagcgag agcgatcgga gccgacacat cgcgtgggac atcggggtgc gagcgctggg
     6361 cgaagcgctg gctgcacgac tggatgcggt gttcatctcc cagacctggt cgcggctggt
     6421 gatcgactgc aaccgcgatc cggaccgcgc cgacgccatc ccggagatca gcgacgggtc
     6481 tcgcattccg ggtaaccagg gtctgacgcc gcaagagcgg gcggtgcgga tcgccgaagt
     6541 ccacgcgccg tatcaagacg ccatagcgtc agagatggcg gaccgcaggc gcatgtccaa
     6601 accaaccgtt ctcgtcgccc tgcacagctt cacaccctcg atggggggct tcgaccggcc
     6661 gtgggatgca ggcgttctgc acgacggcgg cgacctacgg ttcgcccgcg ccatgctgga
     6721 tgtgatgcag gccacgcccg ggctgaatat cggggataac gcgccctacc agatggacgc
     6781 gatagaccac accgtgccgc ggcatgccat tgccgccggt ctaccctacg tagaactcga
     6841 gattcgtcag gaccatctct cgaatgacat cggcgtcaat cgttgggcag actggatcgc
     6901 gggctggctg acgcagtcgg cagccgtcat ggagaacgcc tcgccgtagc cgagttgagc
     6961 cagcggctgc tgagcagttg attcagaggc atcagccgga atgaatcccg tcttccatag
     7021 cgatccggcg catcatctcg atcgccgctg tcggcgagct ccggctttca gccactgcct
     7081 tcctgatagc aggctccatc tcaggtgcga tccggggcgc ggtcatcgac cagtccggaa
     7141 acagccgtcc ctcgacgtgc aatcgggtaa cgatctcgat gtccgtgtgc cggcggtctg
     7201 ccatgacccg gttcatcaaa cggtcgacat cggcagtctg tccctcaatg acctggaaga
     7261 acttgccctc gctaaattcc agcgcgcccg tcagatgatc gcgcgcattg ttccgctgcg
     7321 agaccgcgag aatgtcggtg atcacgagga gagactccag cgagagagtg gcagtgctga
     7381 cataagcgac gcgttcgagc ggtcgggcca tctggaaatc ctggcacgtc ccttcccggc
     7441 aggagagttg ggatcggcgg cagacgaacg tacgctcatt caccgcgttc ctgagtcgac
     7501 gctgcgacac aggcctgaaa gtagggccat ggagcttttg ccgtcgtcga gagttaggcg
     7561 cgctctgggg gacatcatgc gccgatcgat ttcatcacgg accgttctgc acggggcttc
     7621 gcgcctcgga cagggcgtcc tgcgacgcct tgccagcctg ggacagtcag tcggggctct
     7681 acgaggacct ggcaacgcaa gccacggcaa atctcgcggg ggatcatcgc tcagtgtttt
     7741 cgtcgagtat gttgcgcggg tgggctacgg cgctcgtggc ttcgtctatg tctctctggg
     7801 cctgctgacc ttactggccg cgctcgacct cggcggcaga gcggtcggaa ctcacggcgc
     7861 tatcctgtgg ctggccgaac aaccgttcgg gcggggctgg ctgatcgccc tcggcgccgg
     7921 tttgtgggcc ttcgtcgcgt ggcgggtgct tcagtcggtc ttcgacgccg atcgcgaagg
     7981 acgaacgcct cgtgcgctgc tgcttcgcgt gggtcaggcc gtcagcgggc tgttctacgc
     8041 cgtgctgggg tccggcgtgt tcgaactgct ggacgaggtc gggtcgagaa tgggagcgga
     8101 ggacgttgcg gagaaccagc gcaaggctgc gctcttgctc gaccttccct tcggtgacgc
     8161 cctcctgatc tccgttggtc tggtgatcac cgcggtaggg gttggcaata tcgtcaaggg
     8221 gatccgaagc gatttcgccg acagcttggc ctgttcggag cgcctgtgtc ggcggctgac
     8281 cccggtggca cgcgccggct acatcgcgcg aggcgccgcc tacctgccgc tggcgctgct
     8341 cgtcgtcctg gccggctggc atgcccgggc cagcgaggtc gaaagcttcg gttcgtcgct
     8401 ggatgccctt gaggctcaac ccggcggctc gttgatgttg ggtgcgacgg ctgtcggcct
     8461 gatggccttc ggtgtcttcg cattcgtgga ggccaggttc cggcgcatcc gggcgccgcg
     8521 cgacctcaac ccgctccagg caggatcaag tccgttcccg cgttggcagt cgttgcttgc
     8581 ccagcgtttg cggaaaccgt agccggcgac cggttcgacg tctggtccac atcagcgaaa
     8641 gctaccggct ccacgcccgg ctggtgggta agtctgccaa ccacatagac gcccttttcg
     8701 gccagagccc ggttcagccg ctcgcgcaga gtgaagcgta cctcggcatc tatgatctga
     8761 cgtttcctgt ctgggtccat cggagcgtcc tttcgccgct gacttccctc aaaaacgcat
     8821 gccgggcaag agtcggttgc cgtagaagag cgcgcgggat cagggagaga tctatcagcc
     8881 tggcaaagcc ggcatgggat tggcggcttg ctattcctgg aagggcgggt ggctatggga
     8941 gggctcttgt ccttcagata tcacgacggg cgggccgacc gcggcgcact ggtcggaacg
     9001 aaggcgccaa gtgtcggttg ggcttga
//
LOCUS       DASVTM010000071         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_432738,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000071 DASVTM010000000
VERSION     DASVTM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432738"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VF633_01890"
     CDS             complement(<1..641)
                     /locus_tag="VF633_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8469838.1"
                     /translation="MTASVHATEQMLFSILVQLILMIGAARLLNIAFRKMGQPGVIGE
                     IVAGLLLGPSLFGHFFPEFSIDLFGAKAATPIVILSQIGLILLMFQIGSEFQFGHLRT
                     PRNRRAMAVISVASITVPFGLGFAIGQVSHSVLAPGIDPLIYSLFTGVAMAITAVPVL
                     GRILREFGLTKDEMGVVAISAAAVNDVVGWVMLACISAYAAAQFSTGNMLVQLA"
     gene            complement(710..3256)
                     /locus_tag="VF633_01895"
     CDS             complement(710..3256)
                     /locus_tag="VF633_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8469839.1"
                     /translation="MKYAVSQLAVAAAIPLLGLSPLSAASAQTGATDGPAAAQLSSAG
                     PDSTAHPALWPRAASPAAMSDPATEAFVTELMGRMTMEEKVGQTIQADISTITPEDLV
                     RYPLGSVLAGGSSGPWGDDKAPAQKWVDLARAFRAAAAARPGARVPLIYGIDAVHGHN
                     NVPGATIFPHNIGLGAARDPDLIRRIGEATALEVAVTGADWTFGPTLAVPRDDRWGRA
                     YEGYAEDPEVQRAYAGPMTLGLQGELSADHPLAPGHIAGSAKHFVADGGTFEGEDQGD
                     FKGTEQELIDVHLGGYPAAIDAGVLSVMASFSGWNGVKHSGNHSLLTDVLRGPLGFQG
                     FVVSDWNAHGQLPGCTNDSCPLAINAGVDMLMAPDTWKPLYESTLEAARSGVIPTARL
                     DEAVRRILRAKVKAGVFRTDRPIEGDLSRIASPAHRALAREAVRESLVLLKNEGSVLP
                     IRAGARVLVAGAAADDIGQAAGGWTLAWQGTGNTNADFPQGQSIWGGIDEAVKASGGT
                     ATLSADGAFTDKPDVAIVVFGEAPYAEFQGDVDTLDFIPTGPLETLKRLQAAGIPTVS
                     VFLSGRPMWTNPEINASDAFVAAWLPGTEGGGVADVLVGDAQGNARHDFHGVLSFSWP
                     RNAAGAPLNRGQPGYDPQFAYGYGLTYARSGSVGTLSQESGVEAATSNIDHYFVDGRY
                     VAPWGVMLRDAGGQNKPGTVKVASSPRGGVSVRPVDDLAQESGQQFAFSGSGPAQAYI
                     EGTPVDLTRQSNGELALSFRYRIDATPQGSVGLSVGQGRVDVTELFRSAPVRQWRTLK
                     VRLSCLRDAGADVAHVDVPFRLDTDAAFTVSVSEVQLAPNANDTVCPARH"
     gene            complement(3382..>4406)
                     /locus_tag="VF633_01900"
     CDS             complement(3382..>4406)
                     /locus_tag="VF633_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX8469840.1"
                     /translation="RARGCECMKSVREYQNVDRARFETEIVPAGRPAVLRGLVADWQI
                     VRAAHSDENLAGYLRDFDTGKPVRTFVGAPEMSGRFGYSPDLKGFNHDQTAMPLADLL
                     ERLLSGRGQGGPHIYAGGVPLPEVLPGLSEANPMPLLDPGMERLTSLWIGNRSRTAAH
                     WDLPQNLACVVRGKRRFTLFPPEQIANLYVGPLDFTLAGQPISLVDIVEPDFDQHPKF
                     REALEVAEVAELVPGDALYLPSLWWHHVETTADFGAMVNLWWRDGPPWMGTPLLTMFH
                     ALLTIRDLPSDERMRWRSFFDHYIFQTGDDPVAHIPMDARGVLAPMNANLHQRLRALI
                     SRTLQR"
BASE COUNT          794 a         1461 c         1438 g          713 t
ORIGIN      
        1 gccagttgca ccagcatgtt accggttgag aactgagcgg cggcataggc cgagatacag
       61 gccagcatga cccagccgac gacgtcgttc accgctgcgg cggagatggc caccaccccc
      121 atctcgtcct tggtcaagcc gaactcgcgc agtatccgcc ccaagaccgg cacggcggtg
      181 attgccatcg cgacgccggt gaacagactg tagatcaggg gatcaatccc gggcgccagc
      241 accgaatgcg acacctggcc gatggcgaag cccagaccga agggaacggt gatcgaggcg
      301 acggagatga cggccatcgc acgccggttg cgcggagtcc tcaggtgacc gaactggaac
      361 tccgagccga tctggaacat cagcaggatc aggccgatct ggctcaggat cacgatcggc
      421 gtcgcggcct tggctccaaa caggtcgatg gagaactccg ggaagaaatg gccgaagagc
      481 gacgggccga gcaggaggcc agcgacgatc tcgccgatca cccccggctg gcccatcttg
      541 cggaaagcga tgttcaatag tcgggccgca ccgatcatga ggatcagctg gaccagaatg
      601 gagaacagca tttgttctgt cgcgtggacg gatgcagtca cgtctttctc cccctgtgac
      661 ggtcgtgtct tgtgcacgcg ccgtcgtttc tcgttatagc tgttccgccc tagtgtctcg
      721 cgggacaaac ggtatcgttg gcattggggg caagctggac ctccgaaacc gagaccgtga
      781 aagcggcgtc ggtgtcgagc cggaacggca cgtcgacgtg agccacgtcg gcgccggcgt
      841 cccgcaggca cgagagccgc accttcagcg tccgccattg ccggaccggg gccgatcgga
      901 acagctcggt gacatccacc ctgccctgcc cgaccgatag cccgaccgac ccttgcggcg
      961 tcgcatcgat gcggtagcgg aagctgagcg ccagctcgcc gttggactgc cgggtcaggt
     1021 cgaccggcgt tccctcgata taggcctggg ccggaccgga gccggaaaag gcgaactgct
     1081 ggccgctctc ctgggccaga tcgtcaactg gccgaaccga aacgccgcca cgtggactgg
     1141 atgcgacctt gaccgtcccc ggcttgttct gccccccggc gtcgcgcagc atcacgcccc
     1201 aaggggcgac atagcggccg tcgacgaaat aatggtcgat gttagaggtt gcggcctcga
     1261 cgccgctctc ttgcgaaagt gtaccgaccg atccgctgcg ggcataggtc aggccgtagc
     1321 cgtaggcgaa ctgcggatca tagccgggct gaccgcggtt cagcggcgcg ccggcggcgt
     1381 tcctgggcca gctgaacgac aggacgccgt ggaaatcgtg gcgggcattg ccctgcgcgt
     1441 cgccgaccaa cacatcggcg acgccgccac cctcagttcc cggcagccag gcggcgacaa
     1501 aagcgtccga agcgttgatc tcggggttgg tccacatcgg tcgacccgac agaaaaaccg
     1561 aaaccgtggg aatgcccgcc gcctgcagcc ttttcagcgt ctcgagtgga cctgtcggga
     1621 tgaagtcgag cgtgtcgacg tcgccctgaa actcggcata gggggcctcg ccgaagacca
     1681 cgatggcgac gtcgggcttg tcggtgaagg cgccgtcggc gctgagggtc gccgtaccgc
     1741 ccgacgcctt cacggcttca tcgatcccgc cccagatcga ctggccttgt gggaagtcgg
     1801 cgttggtgtt gcccgtgccc tgccaggcca gggtccagcc gcccgccgcc tggccgatgt
     1861 cgtcggcggc cgcgcccgcg acaagcacgc gcgccccggc gcggatcggc agaaccgaac
     1921 cctcgttctt gagcaggacc agcgattcac gcaccgcttc acgcgccagt gcgcggtgcg
     1981 ccggcgaggc gatccgcgac aggtctccct caatcggacg atctgtacgg aacacgcccg
     2041 ccttgacctt ggcgcgcagg atgcgacgaa cggcctcgtc cagacgggcc gtcgggatca
     2101 cccccgatct cgctgcctcg agcgtgcttt cgtacaatgg cttccaggtg tcaggagcca
     2161 tcagcatgtc gacgccagca ttgatggcca gggggcaact gtcgtttgtg cagccgggca
     2221 gctggccgtg ggcgttccag tcggagacta cgaaaccctg aaagcccagc ggtccccgca
     2281 ggacgtccgt cagcaagctg tggttgcccg aatgcttgac cccgttccaa cccgaaaagc
     2341 tggccatgac cgacaggaca cccgcgtcga tggcggccgg atagccgccc aggtggacgt
     2401 cgatcagctc ctgctcggtc cccttgaaat cgccttggtc ctcgccttcg aacgtgccgc
     2461 cgtcagcgac aaaatgcttg gcagatccgg cgatatggcc gggcgccagc ggatggtcgg
     2521 ctgacagctc gccctgcagt cccagcgtca tcggcccggc ataggcgcgc tgaacctcgg
     2581 ggtcttcggc ataaccttca taggcgcgtc cccagcgatc gtcgcggggc accgccaggg
     2641 tcgggccgaa cgtccagtcg gcgccggtga ccgccacctc caacgccgtc gcctcgccga
     2701 tgcgacggat caggtcggga tcgcgcgctg cgccaaggcc aatgttgtgt gggaagatgg
     2761 tcgcgccggg aacgttgttg tgcccgtgca cggcgtcgat accgtagatc agcggcaccc
     2821 tggctcccgg tcgggccgcc gccgcggcgc ggaaggcgcg ggccagatca acccactttt
     2881 gcgcgggcgc cttgtcatcg ccccaagggc cggagcttcc ccccgccaat accgaaccca
     2941 gcggatagcg caccagatcc tcaggcgtga tagtgctgat gtcggcctgg atggtttggc
     3001 cgaccttctc ctccatggtc atccggccca tcaattcggt gacgaaggcc tcggttgcgg
     3061 ggtcgctcat ggccgcagga gaggcggcgc ggggccaaag cgccgggtgt gccgtcgagt
     3121 cgggaccagc gctcgataac tgggctgcgg caggcccatc agtcgctccg gtctgggcgg
     3181 aggcggcgct caagggcgac agacccagca gcggaatcgc tgcggcgaca gccagttggg
     3241 acacggcgta tttcatggat tttcctcgac caagggcgcc cgccccggtc gctcattgag
     3301 cggcgaccgt cccggcaagg aacccgtgaa tggagttgtt gcgcattccc ccagccttgt
     3361 cacccaaaat tgagaacgct ctcaacgttg cagggtgcgg ctgatcaacg ccctcagtcg
     3421 ctggtgcaga ttcgcattca tgggcgcaag cacaccccgg gcgtccatgg gaatgtgggc
     3481 gaccgggtcg tcgccagtct ggaagatgta gtgatcgaag aagctgcgcc agcgcatccg
     3541 ctcgtccgac ggaaggtcgc ggatggtcag cagggcatgg aacatcgtca gcagcggcgt
     3601 gcccatccag ggcggcccgt ctcgccacca cagattgacc atggccccga aatcggcggt
     3661 ggtctcgaca tgatgccacc acagacttgg caggtagaga gcgtcaccgg gaacgagttc
     3721 cgccacctcc gcaacctcca gcgcctcgcg aaatttcggg tgttggtcga agtcgggttc
     3781 aacgatgtcg accagactga tgggctgtcc tgccaaggtg aagtcgagcg gcccgacata
     3841 gaggttggcg atctgctcgg gcggaaacag ggtgaagcgc cgcttgcccc ggaccacgca
     3901 ggccaggttc tggggtaaat cccagtgcgc cgccgtccgc gaccggttcc ctatccacag
     3961 ggacgtcagc cgttccatgc ccggatccag cagcggcatc ggattggcct cggacagtcc
     4021 cggcagaacc tcaggcaagg gcacgccacc ggcgtagatg tgcggaccac cctgccctcg
     4081 cccggacagc agccgctcca gcaggtccgc cagaggcatg gcggtctgat cgtggttgaa
     4141 ccccttgagg tcgggtgaat agccgaaccg cccgctcatc tcaggcgcac cgacgaaggt
     4201 gcgcaccggt ttgccggtgt cgaaatcccg cagatacccg gctagattct cgtcagagtg
     4261 cgcagcgcgg acgatctgcc agtcggcgac caggcccctc agcacggccg gtcgcccagc
     4321 cgggacgatc tccgtctcga accgggcccg gtcaacgttt tggtattcgc gaaccgactt
     4381 catgcactca caacctctgg cgcggt
//
LOCUS       DASVTM010000072        11102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_220432,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000072 DASVTM010000000
VERSION     DASVTM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220432"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VF633_01905"
     CDS             <1..959
                     /locus_tag="VF633_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEX8469841.1"
                     /translation="VRTSGATMFVYIGELCRYLVNCPPGEFDRGHKLKLAFGNGLRPD
                     VWPDFQSRFKIPDILEFYGSTEGNVSLFNFDGKQGAIGRVPKLLKKQINIRLVRFDID
                     TNEPVRAANGLCEEARPGEIGEAIGAIGNDVRHDFSGYADKAASQKKILTDVFARGDK
                     WFRTGDLMRQDSEGYLYFIDRIGDTFRWKGENVSTSEVEQRLAEAPGVKEVIAYGVPV
                     PNTEGKAGMVTLIVEGKFAPQAFADYADAQLPSYARPVFVRVAKTLETTGTFKYRKVD
                     LVTDGYDPAKIEGGVWVRGGKTGYQKLTPAIIAQILSGENRL"
     gene            1047..1913
                     /gene="motA"
                     /locus_tag="VF633_01910"
     CDS             1047..1913
                     /gene="motA"
                     /locus_tag="VF633_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX8469842.1"
                     /translation="MFQIIGIVLLFGLVFGSFLMSGGKMDVILHAAPHELMAILGAGV
                     AAFLIANSMGVIKASLAGFGKCFAGPKWKKQDYKDLLSLLFLLTKTMKSKGVIALESH
                     IEKPGESSIFQKYPKVMKDHFAIDFICDTLRMMTMNLEDPHQIEDAMEKQLEKHHHEA
                     ACAPHALQNLADGLPALGIVAAVLGIIKTMGSITEPPEVLGEMIGSALVGTFLGVFLA
                     YGLVGPFASRLQAIVDEEAAFYKIIQSVLVAHLHGNAAQISVEIGRGDIPSSAQPTFL
                     EMEEALAAAPTE"
     gene            1958..2203
                     /locus_tag="VF633_01915"
     CDS             1958..2203
                     /locus_tag="VF633_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469843.1"
                     /translation="MRARLIRSAFASIVLVGGLSACHSDTHDVRPEEPVSSEGLVSDA
                     GRNPEGITASDTEAAQAGTQAAEAIGVAETSTTRPEK"
     gene            2365..2688
                     /locus_tag="VF633_01920"
     CDS             2365..2688
                     /locus_tag="VF633_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469844.1"
                     /translation="MNIRNMMALSAIAAAALAITACSPKPEEKAADAAADSAAAADTA
                     ADAAATADQAAQDAAAAAGEAASNAAAVGAAPAEGAMAPAGSAMAPAHASEGAMAPAA
                     VPAKH"
     gene            2783..4552
                     /gene="mnmD"
                     /locus_tag="VF633_01925"
     CDS             2783..4552
                     /gene="mnmD"
                     /locus_tag="VF633_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HEX8469845.1"
                     /translation="MLPSDDATADPLPDSGHADLTWSEDGAPRSSRFGDVYFSKDDGL
                     AESRAVFLQGCGLPERWKDRDRFTVAELGFGTGLNIIALLDLWRREGPPTGWLNIFSI
                     EAFPLTADEAARALAAWPDVADIAEALIAVWPTRTPGFHRIDLPEFRATLDLAIGDAK
                     WALSEWQGAADGWFLDGFSPALNPDMWSEPVMDGIAARSAPGARVATFTVAGAVRRGL
                     SERGFEVEKRPGHGRKRERLEAWLPDGAPAQGTPRSVAVIGAGIAGASVARALTALGV
                     SVTVIEAERPGAGGSGFPAALVTPRLDAGDAVIAGFHAQALKRARDLYGATPGAVVAL
                     GVLQLEQAERDRNRFAKVAAQAWWGPEDMKVLDGGLSMAGALTVRPEPVLKAWLDGAA
                     IVTREVAVIERCETGWRILDAEGAVLAEADAVVLTAGWGSAALSPELGLRPVRGQADW
                     VDIDDAPLASAWGGYVAPTGEGFLFGATHDREDVGTDVRPGDTERNRAVLAARLPVLA
                     ARIEGVETQARAAVRATTADRLPVCGALGSSDGLYVLGGLGSRGFCVAPLLGEHLAAD
                     IVGVPSPLPAAFSRRLTPGRFGN"
     gene            4649..5071
                     /locus_tag="VF633_01930"
     CDS             4649..5071
                     /locus_tag="VF633_01930"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEX8469846.1"
                     /translation="MRQSLLVLIPVVGLLIGSAAAPVATTARAQTSESPAPERRCFSE
                     QQIRNFRSSHEGTLYLKVWGSNSGTYQLATSGGCLDLTTAQQVAVTPGLGSGRLCTGD
                     WATVATQGFSGSPQVCRALIQKRLTDAEIAALPGRDRP"
     gene            complement(5094..6002)
                     /gene="accD"
                     /locus_tag="VF633_01935"
     CDS             complement(5094..6002)
                     /gene="accD"
                     /locus_tag="VF633_01935"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX8469847.1"
                     /translation="MSDPKVPSEKPQPEKRGGWLSRFAPGVRKIVSRRADTPDNLWVK
                     DPDSGEMLYRSDLETSLWVTPSGRHMRLNAEQRLRYTFDDGRYEAIDTPDVPEDPLKF
                     SDGKSYKDRLSAARKAAGRKDTMMIGFGDVDGTPAVVIVQDFSFMGGSLGTAAGEAFI
                     AAAHAAIARNVPLVCFTAAGGARMQEGALSLMQMARSTLAVQEVKAAQLPYVVVLTDP
                     TTGGVTASYAMLGDVHLAEPGALIGFAGARVIESTIREKLPPGFQRAEYLQEKGMVDK
                     VVARADLPATLGAILSMLMGGKRRVA"
     gene            complement(6013..6843)
                     /gene="trpA"
                     /locus_tag="VF633_01940"
     CDS             complement(6013..6843)
                     /gene="trpA"
                     /locus_tag="VF633_01940"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX8469848.1"
                     /translation="MTTSRIDARFASLKAEGRAGFVAYVMAGDPSGEESLEILRGLPA
                     AGADIIELGFPFSDPMAEGPPIQRAALRGLKAGLTLRGTLSLAAEFRAGDADTPLILM
                     GYLNPIESYGYEAFARDAAVAGVDGCIVVDCPPEEADPLSDALDAAQVSLIRLATPTS
                     DDARLEIIARRTSGFVYYVSVSGVTGVKEAQAVSVAPAVERVRKASGLPVAVGFGVKT
                     PERAAEIARVADAVVVGSALVDEVALALAANQPVAPRVLAKVKALGDAVRGARVGETV
                     "
     gene            complement(6840..7379)
                     /locus_tag="VF633_01945"
     CDS             complement(6840..7379)
                     /locus_tag="VF633_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469849.1"
                     /translation="MKILSLAAAATLALSSAASAQVVADQTPAAGTPVPWSQTTRTPA
                     PAAAPAPAAQGAPVAAPLDLQIPEITGTAGDPTCGGRPALTQQAFCIQTTQAAMQTVA
                     DQYDAAFQKQGWLAAGGKDNLTVYVKRKDGGGCTGFQLMAFADETRQAAPGAPGYFAF
                     ATIPGDVCAARPETPAAAQ"
     gene            complement(7382..8596)
                     /gene="trpB"
                     /locus_tag="VF633_01950"
     CDS             complement(7382..8596)
                     /gene="trpB"
                     /locus_tag="VF633_01950"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX8469850.1"
                     /translation="MNAIIPNQYAMPDAEGRFGPYGGRFVAETLMPLVLELNTAYEAC
                     KADPTFQAELDSFLTHYVGRPSPLYFAERLTEHHNAANIWFKRDELNHTGAHKINNCM
                     GQILLAMRMGKKRIIAETGAGQHGVATATVCARFGLQCVVYMGATDVERQSPNVFRMK
                     LLGAEVVPVTSGRGTLKDAMNEAMRDWVTNVGDTYYLIGTAAGPHPYPAMVRDFQSVI
                     GKETKVQMMDRRGKLPDALVAAIGGGSNAIGLFHPFIEDAGVRLMGIEAAGRGLDGPD
                     HAASIQGGRPGVLHGNRTYLLQDEDGQILEGHSISAGLDYPGIGPEHAWLKDIGRAEY
                     LAATDAEALEAFQLCAKLEGIIPALEPSHALARVGEIAAEVGKGGDVVLNMCGRGDKD
                     IFAVAKHLGVEL"
     gene            complement(8629..9267)
                     /locus_tag="VF633_01955"
     CDS             complement(8629..9267)
                     /locus_tag="VF633_01955"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX8469851.1"
                     /translation="MTGVKICGLTTVETLDVALDGGASFVGAVVFPKSPRHITPMEAA
                     TLFERARERALIVAVVVNPDDDLLADIAAMLKPDFIQLHGSETPARAEQIRTLTGAGI
                     IKALPIRTHEDFAPAIEWEPFVDHLMFDAKPVEGSTLPGGVGASFDWSLLADRAFRQP
                     WFLAGGLNPANVAEAIRISGAPLVDVSSGVETAPGVKDAGRIAAFLEASRRS"
     gene            complement(9503..10249)
                     /locus_tag="VF633_01960"
     CDS             complement(9503..10249)
                     /locus_tag="VF633_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX8469852.1"
                     /translation="MTKIPTSRVDKLLGSMGYGSRTEIARLGKVGGIVLDGVDLTDVS
                     KRIPVPSDLPSRMAIDGQPLDPVPGLVMLLNKPLGMTCSHKEDGALVYDILPERWKRR
                     DPAISTIGRLDKQTSGLLLLTDDGDLLHRVISPKKHVAKVYRATLARPLVGTEGELFA
                     QGGLVLEGEDKPLAPAHLEVISPNEARLTVTEGRYHMVRRMFAAAGNHVEALHRERMG
                     GLALPDDLAPGEWRLLTPDQIALIFEPESA"
     gene            complement(10284..>11102)
                     /locus_tag="VF633_01965"
     CDS             complement(10284..>11102)
                     /locus_tag="VF633_01965"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8469853.1"
                     /translation="DSTPLENLEPGSIEGAMVYAPPGVLERRYTLALALKALKVGGRL
                     DVMAPKDKGGSRLKKELTAFGVEIGETAKAHHRRCVVIRPEAMTGIEEAIEAGAPRLV
                     EGLEAWSQPGVFAWDRIDGGSALLAEALPPLKGKGADLGCGYGALATVVLRSPAVTSL
                     RLVDMDRRAVEAAKRNVDDPRATFEWTDTRTLEDTSDLDFIVMNPPFHDGGAEDRKLG
                     QAFIRKAAGMLKKGGVLWLVANRHLPYETELKEAFKRVTPVGDGGGYKLFEAMK"
BASE COUNT         1935 a         3669 c         3588 g         1910 t
ORIGIN      
        1 acgtgcgcac cagcggtgcg acgatgttcg tctatatcgg cgaactctgc cgctatctgg
       61 tcaactgccc ccccggtgag tttgatcgtg gtcacaagct gaagctcgcg ttcggaaacg
      121 gtctgcgccc cgacgtctgg cccgacttcc agagccgctt caagatcccc gatatcctgg
      181 aattctacgg ctcgaccgag gggaacgtct cgctgttcaa cttcgacggt aaacaggggg
      241 ccatcggtcg cgttcccaag ctgctgaaga aacagatcaa cattcgactg gtccggttcg
      301 acatcgacac caatgagccg gttcgtgcgg ccaacggcct gtgtgaagag gctcgtccgg
      361 gcgaaatcgg cgaggctatc ggcgcgatcg gcaacgatgt tcgtcacgac ttctccggct
      421 atgcggacaa ggcggcttcg cagaagaaaa tcctcacgga cgtcttcgcg cgcggagaca
      481 aatggttccg aacaggcgac ctgatgcgtc aggacagcga gggctacctc tatttcatcg
      541 accggatcgg cgacaccttc cgctggaagg gcgagaacgt ctccacctcc gaggtcgaac
      601 agcggctggc cgaagcgccg ggcgtcaagg aagtcatcgc ctacggtgtt cctgtcccga
      661 ataccgaggg caaggcaggc atggtcaccc tcattgtcga gggaaagttc gcgccacagg
      721 cctttgccga ctacgccgac gcacagttgc catcctacgc tcgccccgtg ttcgtccggg
      781 tcgccaaaac gctcgagacc acgggcacgt tcaagtaccg taaggtcgat ctggtgacgg
      841 acggttacga tccggccaag atcgagggcg gtgtctgggt gcgtggcggc aagactggct
      901 atcagaaact gacgccagcg atcatcgccc agatcctcag cggcgaaaac cggctctagc
      961 gttcgccgga cggcgcaatc gcaaccgcac cttaatcatt aacggccatg gtcggcgcct
     1021 ctcctgcgga cggcggacgc cttttcatgt ttcagataat cggcatcgtg ctgctgttcg
     1081 gcctcgtgtt cggaagcttc ctgatgtcag gcggcaagat ggacgtaatc ctccacgccg
     1141 ccccgcacga attgatggcc atcctcggag cgggcgtcgc cgccttcctc atcgccaaca
     1201 gcatgggcgt catcaaggcg tcactggcgg gcttcggcaa atgtttcgcc ggtcccaagt
     1261 ggaagaagca ggactataag gacctgctca gcctgctgtt cctgctgaca aaaacgatga
     1321 aatcaaaggg cgtcatcgcg ctcgaaagtc atatcgagaa gccgggcgag tcctccatct
     1381 tccagaaata cccgaaggtg atgaaggacc atttcgccat cgacttcata tgcgacaccc
     1441 tgcggatgat gacgatgaac ctggaggatc ctcaccagat cgaggacgcc atggaaaagc
     1501 agctcgaaaa gcatcaccat gaagcggcct gcgcgccgca cgcgcttcag aacctcgccg
     1561 atggcctgcc ggctctgggt atcgttgcgg ccgtgctcgg catcatcaag accatgggct
     1621 ccatcactga gcctcccgaa gttctcggtg agatgatcgg ttcggccttg gtgggcacct
     1681 tcctcggcgt gttcctggcc tatggtctcg tcggcccgtt cgcgtctcgc cttcaggcga
     1741 tcgtcgacga ggaagctgcg ttctacaaga tcatccagtc ggtcctggtc gcccacctcc
     1801 acggcaacgc cgcccagatc tcggtcgaaa tcggccgcgg cgatatcccc tcttccgccc
     1861 agccgacgtt cctcgagatg gaagaggctc tcgctgctgc tcccacggaa taatggtcaa
     1921 gacatccggc aggtcggccg gatcatcgag gaagacaatg cgcgcccgac tcatccgcag
     1981 cgcctttgca tccatcgtct tggtgggtgg actgtctgcc tgccactccg atacgcacga
     2041 cgttcggcct gaagaacccg tctcttccga gggccttgtg tcagacgccg gacgcaatcc
     2101 cgaaggcatc accgcatccg atacggaggc ggctcaagcc ggtacgcagg cagcagaggc
     2161 tatcggcgta gccgagacca gcaccacgcg cccggaaaaa tagttcgtga tcaatctggt
     2221 cggggcgacg acagtcaagt ctcaccgcat gcaccccgcc atgcgatcac gaacgtgtga
     2281 tatttcgctt gcggtttctc cggcccggtc gttatctggt cgtcgcgagg cttgaagatc
     2341 tcgctgaaca ggaaggaatt ccccatgaac attcgtaaca tgatggcgct ctcggccatt
     2401 gctgcagccg ctctcgctat aactgcctgc tcgccgaagc ctgaagaaaa ggctgccgac
     2461 gccgcagccg actcggccgc tgccgccgac accgcggccg acgccgccgc gaccgccgat
     2521 caagccgccc aggacgccgc cgctgcggca ggcgaagctg cctcgaacgc ggctgctgtc
     2581 ggcgctgctc cggccgaagg cgcaatggcg ccagccggca gcgcgatggc tccggcccac
     2641 gcctccgaag gcgctatggc tccagccgcg gtgcccgcga aacactaagg ccggtaaaca
     2701 gacctcacgg tttgcacgga aaggggtcat cctcgggcga cccctttttc gtatccatgc
     2761 gccatcggtg taggacacgg gaatgctccc ctccgacgat gccaccgccg accccctccc
     2821 cgacagcggt cacgccgacc tgacctggtc cgaagacgga gccccccgct cctcgcgttt
     2881 tggcgacgtc tatttctcca aagacgacgg cttggccgag agccgcgcgg tcttccttca
     2941 ggggtgcggt ctgcccgagc gctggaaaga tcgggatcgc ttcaccgtag cggagcttgg
     3001 gttcggcacc gggctcaaca tcatcgcgct gctggacctg tggcgtcgcg agggaccgcc
     3061 gaccgggtgg ctgaacatct tctccatcga agccttcccg ttgaccgcag acgaagcggc
     3121 gcgcgcgttg gccgcatggc ccgacgtcgc cgacatcgcc gaagccctga tcgcagtttg
     3181 gccgacccgg acgccgggct ttcaccgcat cgacctgccc gagtttcggg cgacgctcga
     3241 cctcgccatc ggtgatgcca aatgggcgtt gtccgagtgg caaggcgcgg ctgacggctg
     3301 gtttctcgac ggcttctccc ctgccctcaa ccccgacatg tggtccgagc ccgtgatgga
     3361 cgggatcgcg gcccgatcag ccccgggtgc gcgggtcgcc actttcaccg tcgccggagc
     3421 ggtgcggcgt gggctatcgg agcgcggctt cgaggtcgag aaacgccccg gccatggtcg
     3481 caagcgcgaa cggctggagg cttggcttcc ggacggcgcc cctgcgcagg gtacgccccg
     3541 gtccgtcgcc gtcatcggcg ccgggatcgc cggcgcctca gtcgcccgag cccttacggc
     3601 gttgggagtg tcggtcaccg tgatcgaggc ggagcgcccc ggggcgggcg gatccggctt
     3661 tccggcggcg ctggtgacgc ctcgcctcga cgccggtgac gcagtgatcg caggcttcca
     3721 cgcgcaggcg ctgaaacgcg cacgcgacct ctacggggcc accccgggtg cggtggtcgc
     3781 cttgggcgtc ctgcaactgg aacaggccga acgcgatcgc aaccgcttcg ccaaggtcgc
     3841 cgctcaggcg tggtgggggc cggaagacat gaaggttctg gatggtggcc tgtcgatggc
     3901 gggcgctctg accgtgaggc cagaacccgt cctgaaagcg tggctcgatg gcgccgccat
     3961 cgtcacccga gaggtggccg tgatcgagcg gtgcgaaacc ggatggcgca tcctggacgc
     4021 cgagggcgcg gtgctggccg aagccgatgc cgtggttctg acggctggct ggggctcggc
     4081 cgcgctgtcg cccgaactgg gcttgcggcc cgttcgggga caggcggact gggtcgatat
     4141 cgacgacgcg ccgttggcga gcgcctgggg cggctatgtc gcgccgacgg gcgaagggtt
     4201 cctgttcggc gccacgcatg accgtgaaga cgttggcaca gacgttcgcc ccggcgacac
     4261 cgagcgcaat cgcgccgttc tcgccgcccg cctgcctgtc ctggcggccc ggatagaggg
     4321 cgtcgaaacg caggctcgcg ctgctgttcg tgcgaccacc gctgatcgcc tgcccgtgtg
     4381 cggcgccttg ggatcgagcg acggcctgta tgtgctcggc ggcttgggat cgcgcgggtt
     4441 ctgcgtcgcc ccgctgctcg gagaacatct ggcggcggat atcgtcggcg tcccctcgcc
     4501 tctgccggcg gcgttctcca gacgcctgac gcccggacgc ttcggcaact gattgtcatc
     4561 accggcaggc cgcctcgctt gtgcccccgg ccagacgagc gcaaaatcga cagctggatt
     4621 gagcccgatc cggtcggagg cgatccccgt gcgtcagtcc ctgctcgttc tcatcccagt
     4681 cgtcggcctg ttgatcgggt cagccgcagc acctgttgcg acgaccgcca gagcccagac
     4741 gtccgagagc cctgcgccgg aacgcagatg tttctcggaa cagcagatcc ggaacttccg
     4801 atccagccac gagggcacgc tttaccttaa ggtctgggga tcgaactcgg gcacctatca
     4861 actggcgacc agcggcggat gcctcgatct gaccaccgcc cagcaggttg cagtcacgcc
     4921 cgggctggga tcaggacgcc tgtgcaccgg ggattgggcg accgtggcga cgcagggctt
     4981 ctccgggtca ccgcaggtct gccgcgccct cattcaaaag agactgaccg acgccgagat
     5041 cgcagccttg ccgggccgcg accggccctg aagccttcag accagacttg aggtcaggcg
     5101 acgcggcgct tgccgcccat cagcatcgac aggatcgcgc cgagggtcgc aggcaggtcc
     5161 gctcgggcga caaccttgtc gaccatgccc ttttcctgaa ggtattcggc gcgctggaag
     5221 cccggcggga gcttttcgcg gatggtgctt tcgatcactc gtgcgcccgc aaagccgatc
     5281 agggcgcccg gctcggccag atgcacatcg cccaacatgg cgtaactggc ggtcacgccg
     5341 cccgtggtcg gatcggtgag gacgacgacg tagggcagtt gagccgcctt cacttcctga
     5401 accgccaagg tcgaacgggc catctgcatc aagctcagcg cgccctcctg cattcgggcg
     5461 ccgccggcgg cggtgaagca gaccagcggc acattgcgcg cgatggcggc gtgggcggcg
     5521 gcgatgaagg cctcaccggc cgccgtgccc aacgacccgc ccataaagct gaaatcctga
     5581 acgatgacga cggcgggcgt cccgtcgacg tcgccgaagc cgatcatcat cgtgtccttg
     5641 cggcccgcag ccttgcgcgc cgcgctgaga cgatccttgt aggatttgcc gtccgagaat
     5701 ttgagcgggt cctcaggcac gtcgggcgtg tcgatcgcct cgtagcgacc gtcgtcgaag
     5761 gtatagcgca gacgctgttc ggcgttcagg cgcatgtggc ggcccgacgg agtcacccac
     5821 agggatgtct cgaggtcgga gcgatacagc atctcgcccg agtccggatc cttgacccag
     5881 agattgtcgg gcgtgtcggc gcggcggctg acgatcttgc ggacaccggg ggcgaagcgg
     5941 ctgagccagc ctcccctctt ctcgggctga ggcttttcgg acgggacttt gggatcggac
     6001 ataggcggct ctctagaccg tttcgcctac gcgcgcaccc cgcaccgcgt cgccgagcgc
     6061 cttaaccttg gccagtacgc gaggggcgac cggctggttg gcggcgagcg ccagagcgac
     6121 ttcatcgacc agagccgagc cgacgacgac cgcatcggcg acgcgggcga tctcggcggc
     6181 gcgttcgggc gtcttgaccc cgaagccgac ggcgaccggc agaccagacg ccttgcgcac
     6241 acgttccacg gccggggcga ccgagacggc ctgcgcctcc ttcacgccgg tcacgccaga
     6301 cacggagaca taatagacga agcccgaggt ccgccgggcg atgatctcca gccgcgcatc
     6361 gtcggaggtc ggcgtggcca gtcggatcag cgagacttga gcagcgtcta gagcatcaga
     6421 caggggatcg gcttcctctg gcgggcagtc cacgacgata cagccgtcca cgcccgccac
     6481 cgccgcgtcc cgcgcgaagg cctcgtagcc ataggattcg atggggttca gatagcccat
     6541 caggatcagg ggggtgtcag cgtcaccggc gcggaactcg gcggccaacg agagcgtgcc
     6601 tctgagtgtc aggcccgctt tcaggcctcg caacgcggcg cgctggatcg gcggcccttc
     6661 ggccatcgga tcgctgaacg ggaagcccag ttcgatgatg tcggcgccgg ccgccggcag
     6721 gccgcgcagg atttccagag actcttcgcc cgaagggtct ccggccatca cataggccac
     6781 gaagccggcc cggccttcgg ccttcagcga agcaaagcgg gcgtcgatac gagaggtagt
     6841 cattgagcag cagccggagt ttctggacgc gccgcacaga catcgccggg aatggtggcg
     6901 aaggcgaaat agccgggcgc gccgggggcc gcctgccggg tctcgtcggc gaaggccatg
     6961 agttggaagc ccgtgcagcc gccgccgtcc ttgcgcttca catagacggt tagattgtcc
     7021 ttgccgccag cggcgagcca gccctgcttc tggaacgccg cgtcatattg atcggcgacg
     7081 gtctgcatcg cggcctgcgt cgtctggatg cagaacgcct gctgcgtgag cgcaggacga
     7141 ccgccgcatg tggggtctcc cgccgtcccg gtaatctcgg ggatctggag atcgagcgga
     7201 gcggcgaccg gagccccctg cgcggccggg gctggagccg ccgctggcgc aggcgtgcgg
     7261 gtcgtctggc tccagggaac gggcgtcccg gcagccggtg tttgatccgc cacaacctgc
     7321 gccgaagcgg cgctggacag ggcgagagtc gccgcagcgg cgaggctcag gattttcatg
     7381 ctcacaactc aacgcccaga tgtttggcca ccgcaaagat gtccttgtcg ccccgcccac
     7441 acatattcag caccacgtcg ccgcccttgc cgacctcggc cgcgatctcg ccgacacgag
     7501 cgagtgcgtg gctgggttcc agagcgggaa tgatgccttc gagcttggcg caaagctgga
     7561 acgcctccag cgcctcggcg tcggttgcag caagatattc ggcccgaccg atgtccttga
     7621 gccaggcgtg ctcgggaccg atccccggat agtcgagacc cgcggagatc gaatgcccct
     7681 cgaggatctg accgtcctcg tcctgaagca gataggtccg attgccatgg agaacgccgg
     7741 ggcgcccgcc ttgaatggaa gccgcgtggt cgggaccgtc cagtccccga cccgccgcct
     7801 cgattcccat caatcgaacg cccgcgtcct cgatgaaagg atggaacagg ccgatcgcgt
     7861 tggatccgcc gccgatcgca gccaccagag cgtcaggcaa cttgccgcgc cggtccatca
     7921 tctgaacctt ggtctccttg ccgatcaccg actggaaatc gcgcaccatg gccggatagg
     7981 gatgcggtcc ggccgccgtg ccgatcaggt aataggtgtc gccgacgttg gtgacccagt
     8041 cgcgcatcgc ctcgttcatg gcgtccttga gcgtgccgcg acccgaggtc accggcacca
     8101 cctcggcgcc caacagcttc atgcggaaca cgttagggga ctgacgctcg acatcggtcg
     8161 cgcccatata gacgacgcat tgcaggccga agcgggcgca gacggtcgcc gtcgccacgc
     8221 catgctgacc tgcgccagtc tcggcgatga tccgcttctt gcccatccgc atggccagca
     8281 ggatctgacc catgcagttg ttgatcttgt gcgcgccggt gtgattcagc tcgtcgcgct
     8341 tgaaccagat gttggccgcg ttgtgatgct cggtcagacg ctcggcgaaa tacagcgggc
     8401 tgggccgtcc aacatagtgg gtcaggaagc tgtcgagctc ggcctggaag gtcggatccg
     8461 ccttgcacgc ctcgtaggca gtgttgagct cgagcaccaa cggcataagg gtctcggcga
     8521 cgaaccgccc gccataaggg ccgaaccggc cctcggcgtc aggcatggcg tactggtttg
     8581 ggatgattgc gttcacggcg gagactctca ggcaagcagt cgtcggaatt acgaccggcg
     8641 cgacgcttcc agaaaggccg cgatccggcc ggcgtcctta acacccgggg cggtttccac
     8701 gccagaggag acgtcgacca aaggcgcgcc ggaaatccgg atcgcctcgg ccacgttggc
     8761 ggggttcaga ccccccgcga ggaaccaggg ctgacggaac gcgcggtccg ccagcaggct
     8821 ccagtcgaag gaagcaccga caccgccagg cagagtcgag ccctcgaccg gcttggcgtc
     8881 gaacatcagg tgatcgacga aaggctccca ttctatcgcc ggcgcgaaat cttcgtgcgt
     8941 gcggatcggc agggccttga tgatgcctgc tccggtcagc gtgcggattt gctcagcgcg
     9001 ggctggggtt tctgagccat gcagttggat aaagtccggc ttgagcatcg cagcgatgtc
     9061 agccagtaaa tcgtcgtccg gattaacgac cacggccacg ataagcgccc gctcccgggc
     9121 tcgctcgaac agggtcgcgg cctccatcgg cgtgatgtgt cgcgggcttt tagggaagac
     9181 caccgcgccg acgaaacttg cgccgccgtc gagtgcaacg tcgagggttt cgacggtggt
     9241 gaggccgcag atttttacgc ctgtcatggg cgaggcaaag gcgaacggcg cttctttgca
     9301 ggtgcctctg tcagaccccg aagaatgacg acctgttcgc cgcgacgaat ggctatccgc
     9361 tcaccacctt cggcgcgcga gatgagttcg gaaaaccgaa cctgggcttc ctcgacagag
     9421 tattccagca tgctgatagt ctagcccatc gcgggctacg cctaaaggcg tatcgttgcc
     9481 ctgaaaaccg ggccaagcag cgttaggcag actcgggctc aaagatcagc gcgatctggt
     9541 ccggcgtcag cagccgccac tctccgggag ccagatcgtc cggcagagcc agaccaccca
     9601 tgcgttcacg atgcagggct tccacgtggt tgccggcggc ggcgaacatg cggcggacca
     9661 tgtgatagcg gccctcggtc accgtcaggc gcgcctcgtt tggggaaatc acttcaagat
     9721 gagcgggggc caaaggcttg tcctcacctt cgagaaccag cccgccttga gcgaacaact
     9781 cgccttcggt gccgacgagt ggacgagcca gagtcgcgcg atagaccttg gcgacatgct
     9841 ttttggggct gatgacccgg tgcagcaggt cgccgtcgtc ggtcagcagc aacagaccgg
     9901 aggtctgttt gtccagccgg ccgatggtcg agatcgccgg atcgcgccgc ttccagcgct
     9961 cgggcaggat gtcatagacc agcgcgccgt cttccttgtg ggagcaagtc atgcccagcg
    10021 gcttgttcag cagcatgacg agccccggaa ccgggtccag cggctgaccg tcgatcgcca
    10081 tacgggacgg caggtccgac ggcaccggaa tacgcttcga cacgtccgtc agatcgacgc
    10141 cgtccagcac gatgcccccg accttgccca gccgcgcaat ctcggtccgc gagccgtagc
    10201 ccatcgaacc gagcagcttg tccacgcgag aggtcgggat cttcgtcatt gcgctatcgt
    10261 ccatcgggct gcttgagcgc gcttcacttc atcgcctcga acagcttgta accgccgccg
    10321 tcgccgaccg gggtcacgcg cttgaaggct tccttgagct cggtctcgta gggcagatgc
    10381 cggttggcca cgagccacag tacgccaccc tttttcagca ttccggcggc cttgcggatg
    10441 aaggcctgac ccagcttgcg gtcttcggcg ccgccgtcat ggaagggcgg gttcatgacg
    10501 atgaaatcga gatcgctcgt gtcttccaac gtgcgcgtgt ccgtccattc gaaggtcgcg
    10561 cgtggatcgt cgacattgcg tttcgcagcc tcaactgcgc ggcgatccat gtccaccagc
    10621 cgcaatgacg ttactgcggg cgagcgcaga accactgtcg cgagcgcacc gtagccacag
    10681 ccgaggtccg cgcccttgcc cttcagaggc ggcagggctt cggccagcag ggcggagcct
    10741 ccatcgatgc ggtcccaggc gaagacgccg ggttgcgacc aagcttccag cccctcgacc
    10801 agacgcggag cgcctgcctc gatggcctcc tcgataccgg tcatggcttc cggtctgatc
    10861 acgacgcagc ggcgatggtg ggccttggcc gtctcgccga tctcgacgcc gaacgccgtc
    10921 agttccttct tgagacgcga gccgcccttg tccttggggg ccatgacgtc gaggcgacca
    10981 ccgaccttca gcgccttcaa cgcgagggcc agggtgtagc gccgctccag cacgccggga
    11041 ggggcataga ccatggcgcc ttcgatcgag ccgggctcga ggttttccag cggcgtggag
    11101 tc
//
LOCUS       DASVTM010000073         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_166680,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000073 DASVTM010000000
VERSION     DASVTM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166680"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..455)
                     /locus_tag="VF633_01970"
                     /pseudo
     CDS             complement(1..455)
                     /locus_tag="VF633_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612205.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            575..1783
                     /locus_tag="VF633_01975"
     CDS             575..1783
                     /locus_tag="VF633_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX8469854.1"
                     /translation="MIRASWTSGTSIEATLELRASSLRAVKGRMRPLFSQERVAASAG
                     AFLDGLLGAERRKTGWMRAEAAGDPGPWRQQAVLGRGRWDADALRDVVRDYALETLAD
                     PDAVLVLDETGFLKQGKASCGVHRQYTGSAGKITNCQIGVFAAYASRHGHAFIDRALY
                     LPKTWTSDPARLAAAHVPEGTTFATKPGLALTMIERAMAAGVPFSWVAADTVYGVGDI
                     GQALRRAGKGYVLGVNSDHAFNSWVGKPPVAGTAEAIANGLAPSAWVRLSAGDGTKGA
                     RLYDWAYCELADLDGAGYDQTGVWTRGLLIRRSLSDGERAYFTTWCPAGTPIAVLVAV
                     EGQRWAIEDAFETAKTELGLTHNETRSWHGWHRHVSLVMMAFALLAVIRHHANAPPPK
                     SRVRPRLRAR"
BASE COUNT          284 a          609 c          581 g          315 t
ORIGIN      
        1 gtcgacgagg acgtgccgct tcttgccctt gatcttcttg ccggcgtcgt agcccgacgg
       61 gtcgatcgca gagccccctt gtccgcgcct ttgacgctct ggctatcgat gatgcccgcc
      121 gtcggactgg cctcccgtcc cgcctgctcc cgacactgca cgaacaacgc gtgatggatc
      181 cgctccagcg tcccgtcgta atcccacaga tcgagatagc cgtggaccgt gctgcgcggc
      241 ggcaagtcct ttgggatcgc acgccactga catcccgtgc tcagcacgta catcagcccg
      301 tcgaccaccg cccgcaggtt cacgctccgc ttgccgccgc cgcgtttacc cggtgggatc
      361 aggggagcga caagcctcca ttcctcatcc gtcagatcgc tcggatagcg caaaccgctc
      421 cgatcgtagc tgccccggtt ctccttcgtc cacatccgca gaggctccta aaagcccctt
      481 gaatcacaag ccgctcctat cgctcaatat gtcttcggac agacactaac attacagttg
      541 cgtttttatc tgagttctga ctctctgcgt caccatgatt cgcgcatcat ggacgtcggg
      601 aacctcgatc gaggcgaccc tggagttgcg ggcctcgtcg ctgcgggcgg tgaaggggcg
      661 catgcggccg ctcttcagcc aggagagggt ggccgcctcg gcgggagcgt ttctggacgg
      721 actgctcggc gctgagcggc gcaagacagg ttggatgcga gccgaagccg cgggcgatcc
      781 tgggccttgg cgtcaacagg ccgtgctcgg gcgagggcgc tgggatgccg acgccctgcg
      841 cgacgtggtg cgggactacg ccctggagac gctggccgac ccggacgcgg ttctggttct
      901 cgatgagacc ggcttcctca agcagggcaa ggcttcctgc ggcgtgcatc gccagtacac
      961 cggctcggcc ggcaagatca cgaactgtca gatcggcgtg ttcgcggcct acgcctctcg
     1021 gcacggtcat gccttcatcg atcgggcctt gtacctgccc aagacctgga cctcggaccc
     1081 ggcccggctg gccgccgccc acgtgccgga ggggacgacc ttcgcgacca agccaggatt
     1141 ggccctgacg atgatcgaac gggcgatggc cgccggcgtg ccgttttcct gggtggcagc
     1201 cgacaccgtg tacggggtcg gcgacatcgg acaagctctg cgccgggcgg gcaagggcta
     1261 cgtgctcggg gtcaacagcg atcacgcgtt caattcgtgg gtcggcaagc cgccggtggc
     1321 cggcaccgcc gaggcgatcg ccaacggcct cgcgccctcg gcctgggtgc gcctctcggc
     1381 cggcgacggg acgaagggcg cacgacttta cgactgggcc tattgcgaac tcgccgacct
     1441 cgatggtgcc ggctacgacc agaccggtgt gtggacgcga gggctcctga tccggcgcag
     1501 cctgagtgac ggcgaacggg cctacttcac gacctggtgc ccggccggca cgccgatcgc
     1561 ggtcctggta gcagtggaag ggcagcgttg ggccatcgag gacgcgttcg agacggcaaa
     1621 gaccgagctc gggctcactc acaacgagac ccgctcctgg catggttggc atcggcatgt
     1681 cagcttggtg atgatggcct tcgccctgct ggcggtgatc cgccatcacg ccaacgcgcc
     1741 acccccaaaa agccgagttc ggccgaggct gcgcgcccgc tgatccgct
//
LOCUS       DASVTM010000074        12414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_699675,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000074 DASVTM010000000
VERSION     DASVTM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699675"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF633_01980"
     CDS             <1..206
                     /locus_tag="VF633_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX8469855.1"
                     /translation="IPDDLQAAIDADAEARVTFATLTAQNRFALIFRMQSLKTEAGRH
                     KAIARLVEMLARGETFHPQGKSQ"
     gene            230..1024
                     /gene="folP"
                     /locus_tag="VF633_01985"
     CDS             230..1024
                     /gene="folP"
                     /locus_tag="VF633_01985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX8469856.1"
                     /translation="MGIVNVTPDSFSDGGRLATVEAAVAHAMRLVEQGADVLDIGGES
                     TRPGAEPVNAAEEIARTAPVIEAIRARWDGPLSIDTLKPEVAEAAVSVGATMWNDVSA
                     LGFSPDSLRTAVTLGCDVVLMHMKGEPRTMQAAPTYDDVVAEVVSYLAERAQAAIAAG
                     VARERIWLDPGIGFGKTVAHNLTLTARLDALVTLGFPVLYAASRKRTIQTVDPTATDP
                     ADRLGGSLALALEGVRRGARMVRVHDVRETVQALRLQAAVEAAGVN"
     gene            1067..1894
                     /gene="thiD"
                     /locus_tag="VF633_01990"
     CDS             1067..1894
                     /gene="thiD"
                     /locus_tag="VF633_01990"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX8469857.1"
                     /translation="MVVESRIAPLRIIPGRVLIVAGSDSGGGAGIQADIKAVTMMGAY
                     AATAITAITVQNTLGVHGVFPLPLDLIEQQARAVLDDIGADAIKTGMLGTVEVVERVA
                     AILDTSHAAVVVDPVMVAKGGASLLASDAIEAVRSLMIPRATLLTPNAPEAEAMTGLT
                     VIDLDGQRRAGEALLAMGAKAVLMKGGHVHSDTVIDVLMTSTGETLLEGPRIDTTSTH
                     GTGCTLASACAAGLAKGLPLEVAVAEAWAYVAEAIRLAPGLGQGHGPLDHGWPLGKR"
     gene            1891..2463
                     /locus_tag="VF633_01995"
     CDS             1891..2463
                     /locus_tag="VF633_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8469858.1"
                     /translation="MTELENRLVTIVRGYPDLMTVLATVRSLELPDWLVFSGSVYQSV
                     WNDVTGRVPGHGVNDFDLGYFDHDTSWDAEDAVIQRVAAAFDEPLRSRVEARNQCRVP
                     LWFKDKFGEDYGPLASTADALPRFVAPAFAVGVRLEADDSITVVAPFGLEDVFDMVIR
                     PNPLRPLAKDWAKVTAKAKARWPELTVLTA"
     gene            2478..3290
                     /locus_tag="VF633_02000"
     CDS             2478..3290
                     /locus_tag="VF633_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX8469859.1"
                     /translation="MRQWTIDAFADRPFMGNPACVVEPFDVWPEDVWLQAMAMENNQS
                     ETAFLRRTPDPARFDLRWFTPAMEVDLCGHATFASGAALLVEMALEADAVTFDTRSGP
                     LVVRRLHDRFEMDLPADPPRRSAEVAGLAEALGEEPVEVWSGRYLVAILADAATVRRC
                     DPDLKALARFEAQGGESGQVVIAAVSDETDVDVVDRFFGPGCGIDEDPATGSAHCILA
                     PLYADKLGRGIVRFRQVYPGRGADFETEVVGDRVLIRGRAVTTIESRLRLRP"
     gene            complement(3357..3749)
                     /locus_tag="VF633_02005"
     CDS             complement(3357..3749)
                     /locus_tag="VF633_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469860.1"
                     /translation="MFKKMIVPALVLAATSVAAMPAAAQSYHGQGHGSNRPPVVSAGY
                     GNWQSINQRQANLDRRIDVGIRNGSLSRREATRLRSEFNSLSRLEASYRRGGFSGWER
                     ADLDRRFDRLAAQIRNERHDRDDRRGRG"
     gene            4008..4367
                     /locus_tag="VF633_02010"
     CDS             4008..4367
                     /locus_tag="VF633_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469861.1"
                     /translation="MPHFMIRARDILLAFLIAAAPLGGAVQAASLPGILLGVQDVIPV
                     LQIDGQRRDKAREQAEDGQRMSLGDIVRRVQSGREGRMLGVRPQGDAYVVRWEYPGGR
                     IVDITVDARSGRILGER"
     gene            4371..5051
                     /locus_tag="VF633_02015"
     CDS             4371..5051
                     /locus_tag="VF633_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8469862.1"
                     /translation="MRILLVEDDADLSRQLKGALSDAGYAVDHAADGEEAHFLGDTEP
                     YDVVVLDLGLPKIDGVSVLERWRRDGKTTPVLILTARGAWSDKVSGFDAGADDYLTKP
                     FHTEELLARLRALLRRSAGIASATLSCGGLRLDPRAARASVNGEPLRLTSLEYRLLHY
                     MMMHQGRVISRTELVEHLYDQDFDRDSNTIEVFIGRVRKKVGSDRIETVRGLGYRLTP
                     LPGETVEA"
     gene            5077..6540
                     /locus_tag="VF633_02020"
     CDS             5077..6540
                     /locus_tag="VF633_02020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8469863.1"
                     /translation="MTDTSIDTAVVVPDTADLATPKALFSRWRLRPGRSLTRRLILLA
                     SAWIAAALIVTGFVLTSQFQESGLRRLGGVLNTTVDTLVAKTTATDTGIVTPQIVDQR
                     TLDLASGKYWSTGEFAPNGQIVILNRSLSIWDSDIVIPRDLPPRLRDAPGQTITYNAI
                     GPIGEPLRVAATVKVLPGRSQPVVFLAAIDRSTIDADTRDFATLTWISLALLGFGLMA
                     AVFIQVRIGLAPLYDLRVEIANVSKGKAARIERAYPVEIAPLAEQVNQLLDHNQETVE
                     RQRTHVGNLAHALKTPLSVMLAEAEGNNDPLANLVRRQTEVMKGQVDHHLRRARAAAR
                     AAHGLGERTPVGEVVDELAVMLERVFQAKDVEIDWRAPDELGFLGERQDLQEILGNLI
                     ENACKWCKRRVRVSAGASGLGHMIVVVEDDGPGIPLEQRETAMKRGQRMDEGAPGSGL
                     GLSIVDDLTRAYGGRLTLSASDLGGLKAILELPAAEL"
     gene            6623..7978
                     /locus_tag="VF633_02025"
     CDS             6623..7978
                     /locus_tag="VF633_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469864.1"
                     /translation="MAELSVAHRMALGQLIGGVPDRTLRMLSLAVAGMPGDKARDLEL
                     MLSQESIHRARRARGFGALAPLFHPRPDGVEAMTFPAGVLPRLWNLAAAREQDLLPLL
                     DTDDDREASRTAAVCGRLYASAAAAVRDQPEAVWPAGLCDPESRARGLIDLGHACDFG
                     GLVHRALPSLKAWVGRPDGDQLAELRLLIRDASAMTSDGAQRLLDILFAHLADAALVL
                     RLVVHTSSAAAREAFLSESELATFVERLIAGVQVRAQRVSAFQPGHPIASLRADLIWC
                     ADVLQELDATIHLDREGPWGKTLRDARVRIGKRLGELLSGVTRVVDKTTPMQKVQTAG
                     RMTRAVPRLEIAIEPKLVQAATDALAMVALLRNLAAPFGCEAQRGALIQSLTVQLSEY
                     GDLVIEEVNAGDAPEETPALERAAMAARFLEQIGAVTEARAVRRRAAVAGGPAPMKRS
                     A"
     gene            8085..8906
                     /gene="ccmI"
                     /locus_tag="VF633_02030"
     CDS             8085..8906
                     /gene="ccmI"
                     /locus_tag="VF633_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX8469865.1"
                     /translation="MTALVTAMAALLVLTGARRGVDVVVDGEPDAAARELAELDRLKA
                     RGLLTEDAWASARAEAGRRLLATRTGPVELHSRPTDRRWVLAGLILTAVAALGLYWII
                     GKPGYGDQTYDRRVEAWAQSVEPLEPEQVAAVLTREARLRPDDRQMLTMLGSARFAAD
                     DPIGAASAFRRALAIDPNDARTWSRLGESLVRSQDGAVGADAEAAFREALKRDPGQLG
                     ALYFLGDAALKRADLAGATILWTPLIKALDPGDPRRIDLERRLATATTTSLGAAG"
     gene            8992..9474
                     /gene="ccmE"
                     /locus_tag="VF633_02035"
     CDS             8992..9474
                     /gene="ccmE"
                     /locus_tag="VF633_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX8469866.1"
                     /translation="MSWLPRSPKARRRLWVVAAVAPILALAVGLSLWAMNDSVTFFFT
                     PSEVTAEKAPAGRDIRLGGLVEAGSVQRHGADVSFGVTDNAATTRVIFRGDLPDLFRE
                     GQGIVAQGAFQPDRTFVAHQVLAKHDEKYMPREVADKLKAKGEWRPEASAAATGTSTL
                     "
     gene            9471..11477
                     /locus_tag="VF633_02040"
     CDS             9471..11477
                     /locus_tag="VF633_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX8469867.1"
                     /translation="MISEFGAYALILALVLAVLQTLLAAAGRIRRSPVLAGAAQGAAV
                     ACAGVVALAFLTLIFAFVTSDFSVANVANNSHTDKPMLYKVAGAWGSHEGSLVLWCLV
                     MTGFGAALARAPSSTGGLPFGLKTSAVAVQGLLGSLFLAFAVFTSNPFARLDPAPFQG
                     ASLNPLLQDPALAFHPPLLYAGYVGFSVTFSLAVAALIEGRVDPAWGRWVRPWALASW
                     VLLTVGITLGSFWAYYELGWGGWWFWDPVENASFMPWLAGAALLHSAVVTERRGALAG
                     WTVFLALLAFTFSMLGAFLVRSGVLTSVHAFAVDPERGLMLLGILGVTAGAAFALFAW
                     RAPKLTGGGLFAPISREGALVLNNLFLTAAAATVLLGTLYPLILQAATGATISVGPPY
                     FALTFTPLMVVAFLILPAGPLLAWKRGDLKGAGQRLAIAAGLALLCALAGWLAFEPRK
                     AIAAGGIAIGTWLILGSLAEVVERVRLFRVPLAEVGRRAKALPLGAWGMTLAHTGLGV
                     FVLGAVIETGFKVEAAAALSLGGTVKAGHYSVRLDQVHIVEGPNYLAERGHLTVTPDH
                     GGRPEVVVAERRFFPAGGQTTTEVGLDFRGLDDVYVVLGERRATPQAAQAWTVRVYWN
                     PWARLIFLGPLIMALGGVLSLLDRRLRIGVAGRRKTVETVESVA"
     gene            11474..11971
                     /locus_tag="VF633_02045"
     CDS             11474..11971
                     /locus_tag="VF633_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX8469868.1"
                     /translation="MRRAALTFAFALLATTALAEPAAAPDRPLTSPSQEARAQTLFQD
                     IRCVVCQHESIADSPAEIAADMRRLVREEIASGSSDQAIRADLVRRWGDYILFKPPVN
                     IATLLLWFGPLLLVLLAGGAFIALSFRGKPVETPLSPDEERRLAELVHPDPLRPYPDA
                     TSSHD"
     gene            12084..>12414
                     /locus_tag="VF633_02050"
     CDS             12084..>12414
                     /locus_tag="VF633_02050"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX8469869.1"
                     /translation="MLKRKEFILGAAAGLIIAAGATAGGVVSWPSAQAEGRPGQLMAS
                     AGAAGLAFAPPQGAPLSFADIVQQVAPAVVQIEVETPVERPQGIQGFQIPGLPGIVIP
                     GPGSQQAD"
BASE COUNT         1893 a         4176 c         4121 g         2224 t
ORIGIN      
        1 ccatccctga cgatctgcaa gcggccattg atgcagacgc tgaggcccgc gtgacctttg
       61 ccaccttgac cgcccagaac cggttcgcgc tgatctttcg catgcaatcg ctgaagaccg
      121 aggcggggcg gcataaggcc atcgcacggc tcgtcgagat gctggcgcgg ggcgagactt
      181 ttcatccgca aggcaagtcg cagtgagcgc tttgacacgg cctctcgtca tggggatcgt
      241 caacgtcacg cccgacagct tctcggatgg cgggcggctg gcaacggtgg aggcggcggt
      301 cgcccacgcg atgcggctgg tcgaacaggg cgcggacgtc ctcgacatcg gtggtgaaag
      361 cacccgtccg ggcgctgaac cggtcaatgc cgcagaggaa atcgcgcgca cggccccagt
      421 gatcgaagcc attcgggcgc gttgggacgg ccccctcagc atcgataccc tcaagccaga
      481 agtcgccgaa gctgcagtct cggtcggcgc aacgatgtgg aacgacgtct ccgcgctcgg
      541 cttttcgcca gacagtctca ggacggcggt gacgttagga tgcgacgtcg tcctcatgca
      601 catgaagggc gagccgcgca ccatgcaggc cgcgcccacc tatgacgacg tcgtggcgga
      661 ggtcgtctcc tatttggccg agcgggctca ggcagccatc gcagcgggcg tcgcacgcga
      721 gcggatatgg ctcgatccgg gcatcggctt tggcaagacc gtcgctcaca acctgaccct
      781 gacggcgcgg ctggatgccc tcgtgacgct cggttttccc gtcctttacg cagccagtcg
      841 caagcgcacc atccagaccg ttgacccgac cgccaccgat cccgcagacc ggctgggcgg
      901 ctcgctggcc ctcgcgctcg aaggagtccg gcgtggcgcc aggatggtcc gggtccacga
      961 cgtacgcgaa accgttcagg cgttgaggct tcaggcggcg gtcgaagcgg caggcgttaa
     1021 ctaacgtccc tcttgccggt tcggtgcggc tgacgcattt gaacgcatgg ttgtcgaatc
     1081 ccgcatcgcg ccgctgcgca tcatacccgg ccgcgtcctc atcgtcgccg gttcggactc
     1141 tggcggcggt gcgggcattc aggcggacat caaggcggtg acgatgatgg gcgcctacgc
     1201 ggcgactgcc atcaccgcca tcacggtgca gaacaccctc ggcgttcacg gcgtcttccc
     1261 cctgccgctc gacctgatcg aacagcaggc gagggccgtt ctggacgaca tcggcgccga
     1321 cgcgatcaag accgggatgc tcggcacggt cgaggtggtg gagcgtgtcg ccgccatcct
     1381 cgacacctcg cacgccgccg tggtcgtcga tccggtcatg gtggccaagg gcggcgcgtc
     1441 gctgctggcg tccgatgcga tcgaggcggt gcggtcgttg atgatccccc gcgcgaccct
     1501 gctgaccccc aatgcccccg aggccgaggc catgaccgga ttgacggtca tcgatctgga
     1561 tggccagcgt cgggccggcg aggccctgtt ggcgatgggc gcgaaagccg ttctgatgaa
     1621 gggcgggcat gtgcacagcg acacggttat cgacgtgctg atgacttcca ccggcgagac
     1681 cctgctggag gggccgcgca tcgacacgac ctcgacccac ggcaccggct gcaccttggc
     1741 cagcgcctgt gcagccggtc tggccaaggg gctgccgctc gaagtcgccg tcgccgaagc
     1801 ctgggcctat gtcgccgagg ctatccggct cgctcccggt ctgggccaag gccacggacc
     1861 gctcgaccac ggctggccat tggggaaacg atgaccgaac tcgagaaccg gctggtgacg
     1921 atcgtccggg gctatccgga cctgatgacc gtcctggcca cggtccgctc gcttgagctt
     1981 cccgattggc tggtgttctc gggctcggtc tatcagagcg tctggaacga cgtcaccggt
     2041 cgcgtacccg gccatggcgt caacgatttc gacctcggct atttcgacca tgacacctca
     2101 tgggacgctg aggacgccgt gatccagcgc gtcgccgccg ccttcgacga accgctgcgc
     2161 agccgcgtcg aggcccgcaa ccagtgccgc gtgccactct ggttcaaaga caagtttggc
     2221 gaggattatg gcccgctcgc atcgacagct gacgcgcttc cccggttcgt ggcgccggcc
     2281 ttcgccgtcg gggtccggct ggaggctgat gacagcatca ccgtggtcgc gccgttcggt
     2341 ctggaagacg tgttcgacat ggtcatacgc cccaaccccc tgcggccact ggccaaggac
     2401 tgggcgaagg tgacggccaa ggcaaaagcg cgctggccgg aactcaccgt tctcaccgcc
     2461 tgaggagacg cccacccatg cgccagtgga ccatagacgc ctttgcagac cgcccgttca
     2521 tgggcaatcc cgcctgtgtg gttgagcctt tcgatgtctg gccggaagac gtctggctgc
     2581 aagcgatggc catggaaaac aaccagtccg agaccgcttt cctgcgtcgg acgccggatc
     2641 ccgcgcgctt cgacctacgc tggtttacgc cggctatgga ggtggatctg tgcggtcacg
     2701 cgaccttcgc ctcaggcgcg gccttgctgg tcgagatggc gctcgaagct gacgccgtca
     2761 ctttcgacac ccgatccgga cctctggtgg tgcgcagatt gcacgaccgg ttcgagatgg
     2821 atctgcccgc cgatccgcct cgccgctcgg cagaggttgc gggcttggcc gaagcgcttg
     2881 gcgaggagcc ggtagaggtt tggtccgggc gatacctcgt cgccatcctc gctgatgccg
     2941 cgacggtgcg tcgatgcgat ccggacctga aggctttggc gcggttcgag gcccaaggag
     3001 gggagagtgg ccaggtcgtg atcgccgccg tgtccgacga aaccgatgtc gatgtcgtcg
     3061 atcgcttctt tggtcccggc tgcggcatcg acgaagaccc ggcgaccggt tccgctcact
     3121 gcattctcgc gccgctctat gccgacaagt tgggtcgtgg aatcgtgcgg ttccggcagg
     3181 tttatccggg acggggcgcc gattttgaga ccgaagtcgt cggagaccgt gtgctgatcc
     3241 ggggccgtgc agtgacgacg atcgagagcc ggttgcggct gcgcccctga tcctgaaacg
     3301 gcgaagggcg cgaccctcgc ggatcgcgcc ccatgactgg acttcagagg tacggtttag
     3361 ccgcgaccac ggcggtcgtc gcgatcgtgc cgttcgttgc ggatctgggc cgcgagacgg
     3421 tcgaagcgac ggtcgaggtc ggcgcgttcc caaccgctga agccaccacg gcgatagctg
     3481 gcctcaagcc gcgagaggct gttgaactcg ctgcgcagac gggtcgcttc acgacggctc
     3541 agcgaaccgt tgcggatgcc gacatcgatg cggcggtcaa gattggcttg gcgctggttg
     3601 atcgactgcc agttgccata gccggccgaa acgacggggg ggcggttcga gccgtggcct
     3661 tgaccgtgat acgactgggc ggcggcgggc atggcggcga ccgacgtagc ggccagaacg
     3721 agggcgggga caatcatctt cttgaacatc ggatcactcc ttggagttct gttcgtcggg
     3781 ggccggggtg gccagcgatg actgcagata accacccccg gcctgagccc gacctgaaca
     3841 gcgcgggtca ggttcggttc atcatcatga caaatgcgaa aggtggcttg gcggtcatgg
     3901 cgcatgacgg atcgtcctcg cgcgcgagca tggttctccg cctgcccttc ccatcagggc
     3961 tgcagtcctt aatgtggcgt tcagacgccg ttcagagcgt tccctgtatg cctcatttca
     4021 tgatccgcgc tcgcgacatc cttctggcct ttctgatcgc cgccgctcct ctcggcggcg
     4081 cagttcaggc tgcttccctc ccggggattt tgctcggggt gcaggatgtt atcccggtcc
     4141 tgcaaatcga cggacagcgc cgcgacaagg cgcgtgaaca ggccgaagac ggccaacgga
     4201 tgagcctcgg tgatatcgtg cgccgggtgc aatccggtcg cgaaggccgt atgctgggcg
     4261 tccggcctca gggcgacgcc tatgtggtgc gctgggaata tcccggcggg cggatcgtcg
     4321 atattaccgt cgacgcccgt tcgggccgta tcctcggaga acgttgaacc atgcgaattc
     4381 tgcttgtcga ggatgacgct gacctgtcgc gccagctgaa aggcgccctg agcgatgccg
     4441 gctatgcggt ggatcatgcg gccgatggcg aagaggcgca tttccttggc gacactgaac
     4501 cctatgacgt cgtggtgctc gacctcggcc tgccaaagat cgacggcgtt tcggtgctcg
     4561 agcgctggcg gcgcgacggc aagacgacgc ctgtcctgat ccttaccgcg cgtggcgcat
     4621 ggtcggacaa ggtctcgggc tttgacgccg gcgccgacga ttacctgaca aaaccctttc
     4681 acaccgaaga gctgctcgcg cgcctgcggg ctctgctgcg gcgctcggcc gggatcgcct
     4741 cggcgacgct ttcctgtggc ggtctgcggc tcgatccccg tgcggcccgg gccagcgtca
     4801 atggcgagcc cctgcgtctg acgtcgctcg aataccgact gctccactac atgatgatgc
     4861 atcagggccg ggtgatcagc cgcaccgagc ttgtcgagca cttgtacgat caggatttcg
     4921 accgcgactc caacaccatc gaagtcttca tcggccgggt gcggaagaag gtcggctcag
     4981 accggatcga gaccgtgcgc gggttgggtt atcgcctgac cccgttgccc ggcgagaccg
     5041 tcgaggcgtg agcgccacgg ccagcaagcc atggcgatga cggacacgtc catcgacacg
     5101 gctgtcgtcg ttcctgatac tgccgatctc gccacgccca aggccttgtt ttcccgctgg
     5161 cgccttcgac ccgggcgctc tctcacacgc cgcctgatcc tgctggcctc ggcctggatc
     5221 gcggcggcgc tgatcgtcac cggcttcgtc ctgacctcgc agtttcagga atccggcctg
     5281 cgccggctgg gcggcgtgct caacaccacg gtcgataccc tcgtcgccaa gactaccgcg
     5341 accgacaccg gcatcgtcac gccccagatc gtggatcagc ggacgctcga cctcgcctct
     5401 ggcaaatact ggtcgacggg agagttcgca cccaacggcc agatcgtcat cctgaaccgg
     5461 tcgctgtcga tctgggacag cgacatcgtc attccgcgcg acctgccgcc gcgccttcgc
     5521 gacgcccctg gccagactat cacctacaac gccatcggtc cgatcggaga gccgttgcgg
     5581 gtcgcggcca cggtcaaggt cctgccaggg cgctcacagc ccgtggtctt tcttgcagcc
     5641 atcgaccgct cgaccatcga cgccgacacg cgcgacttcg ccacactgac atggatatcg
     5701 ctggcgctgc tgggcttcgg cctcatggcg gcggtgttca ttcaggtgcg gatcggtctc
     5761 gccccgctgt atgatctgcg ggttgaaatc gccaatgtca gcaagggcaa ggcggcgcgg
     5821 atcgagcgcg cctatccggt cgagatcgcg cctctggcgg agcaggtgaa ccagctgctg
     5881 gaccacaatc aggaaacggt cgagcgtcag cgaacccatg tcggcaacct ggcccacgcc
     5941 ctgaagacac ccctttcggt catgctggcc gaggctgagg gaaataacga tccgctggcc
     6001 aacctcgtcc gtcgccagac cgaggttatg aaaggccagg tcgaccacca tctgcgtcgc
     6061 gcccgcgccg ccgcccgcgc ggcccacggt ctgggcgaac gcacccccgt gggcgaggtg
     6121 gtcgatgaac tggcggtcat gctggagcgc gttttccagg ccaaagacgt ggagattgac
     6181 tggcgcgcgc cggacgagct tggcttcctc ggcgaacggc aggacctgca ggaaattctc
     6241 ggcaatctga tcgagaatgc ctgcaaatgg tgcaagcggc gtgtccgggt ctcggctggt
     6301 gcgagcggcc tcggacacat gatcgtggtg gtcgaggacg acggtccggg cattccactg
     6361 gaacagcgag agacggcgat gaagcgcggg cagcggatgg acgagggcgc gccgggctcg
     6421 ggtctcggcc tgtcgatcgt cgacgacctg acccgggcgt acggggggcg gctgacgctg
     6481 tccgccagcg acctcggggg cttgaaagca atcctcgagc tgcctgccgc tgagctctga
     6541 tcttcgtcca tcgctggacg aagcattaac ccggcggttc taatctattg gttggaacgg
     6601 tgtttccgcg aggtagagtt ttatggccga gttgtcggtc gcccatcgca tggccctggg
     6661 ccaactgatc ggaggcgtgc cggatcgcac gctgcggatg ctgagcctcg ccgtcgctgg
     6721 catgccgggc gacaaggcgc gcgatctcga actgatgctg tctcaggaga gcatccaccg
     6781 cgcccgccgc gcgaggggat tcggagcgct tgctcccttg ttccacccgc gtccggatgg
     6841 cgtggaagcg atgacctttc ccgccggcgt cctgccgcgt ctctggaatc tcgctgccgc
     6901 ccgagaacag gatctgttgc ccctgctgga cactgacgac gatcgggagg ccagccgtac
     6961 tgcggccgtc tgcggccgtc tgtacgcctc ggcggccgcc gccgttcgtg accagcctga
     7021 ggccgtctgg cccgcaggcc tctgcgatcc cgagtcacgc gcccggggtc tcatagatct
     7081 gggccacgcc tgcgacttcg gagggcttgt ccatcgcgcc ctgccgtcgc tcaaggcatg
     7141 ggtcggtcga cccgacggcg atcagctcgc cgagctgagg ctgttgatcc gcgacgcttc
     7201 ggccatgacg tcggatggcg cacagcgcct gctcgacatc ctgttcgctc atctggccga
     7261 tgccgcgctg gttctgcgac tggtcgttca cacgtcgagc gccgccgccc gagaggcctt
     7321 tctatccgaa agcgagctgg cgacgttcgt cgaacggctg atcgcgggtg tccaggtacg
     7381 cgctcagcgg gtctccgcct tccaacccgg ccacccgatc gccagtctgc gggccgatct
     7441 gatctggtgc gccgacgtcc ttcaggagct cgacgccacg atccatctgg atcgtgaagg
     7501 gccttggggg aagacgctgc gtgatgcgcg ggtgcggatc ggcaagcgtc ttggcgaact
     7561 gctcagcggt gtgacccggg tggtagacaa gaccacgccg atgcagaagg tccagactgc
     7621 gggtcggatg acgcgcgccg tgcccaggct ggagatcgcg atcgagccga aactcgtgca
     7681 ggcggcgact gacgccctgg ccatggtcgc cttgttgcga aatctggccg cgccgttcgg
     7741 ttgcgaggcc caacgtggcg ccctgatcca gtcgctgacg gttcaactga gcgagtacgg
     7801 cgatctggtt atcgaggagg tcaatgccgg cgacgctccc gaggagaccc cggcgctcga
     7861 acgggccgcg atggcggccc ggttcctcga gcaaatcgga gcggtgaccg aagcacgcgc
     7921 ggtgcgccgt cgcgctgcgg tcgcgggcgg tcccgcaccc atgaagcgtt cggcgtaaag
     7981 tccggacgga gatcccgccg aaagagctgg ccagaagacc gttacggacc tcgccgccgc
     8041 gcgcgcgatc cgctactacc ccatacatga cggccttctg gatcatgact gcactggtga
     8101 ccgcgatggc ggcgctgctg gtgctgaccg gcgcacgccg tggcgttgac gttgtcgtcg
     8161 atggcgagcc ggacgccgcc gcccgagaac tggccgagct ggatcgcctg aaagcgcgtg
     8221 gcctgctcac agaggacgct tgggcttctg ccagagcgga ggccggacgt cgtcttctgg
     8281 cgacgcgtac cgggcccgtc gaactgcact cccgaccaac cgaccgccgc tgggtgctgg
     8341 cgggcctgat cctgacggcg gtggccgcgc tcggcctcta ctggatcatc ggcaagcccg
     8401 ggtatggcga ccagacctat gacaggcggg tcgaggcctg ggcccaatcc gtcgagccgc
     8461 tggagcctga acaagttgcg gcagtcctga ctcgcgaggc caggctgcga cccgacgacc
     8521 gccagatgct gaccatgctg ggctccgcgc ggttcgccgc cgacgacccg atcggcgcgg
     8581 cgtccgcgtt tcgccgggct ctggccattg atcccaacga cgcccgtacg tggtcgcgtc
     8641 tgggcgaaag cctcgtccgc tcgcaggatg gagccgtcgg cgccgacgcc gaggccgcct
     8701 tccgtgaagc cctgaagcgc gatccgggtc agctcggcgc cctttatttc ctcggcgacg
     8761 ccgccttgaa gcgcgccgat ctggccggag cgacgattct ctggacgccg ctgatcaagg
     8821 cgctggatcc cggtgatcca cgccgcatcg atctcgagcg ccgccttgcc acggcaacca
     8881 ccacttcgct gggcgcggcc ggatgacgct gctgacgagc gcctcttgga gcgcctcacg
     8941 ccggacgcta tacgaccgcg aatatccctc cgcgggcgga cccttcctga catgagctgg
     9001 cttcccagat cgcccaaggc gcgccgtcgc ctgtgggtcg tcgccgccgt cgcgccgata
     9061 ctggcgctgg ctgtcggcct gtcgctgtgg gcgatgaacg acagcgtcac cttcttcttc
     9121 acgccctccg aagtgaccgc tgaaaaggcg ccggccgggc gcgacatccg gctgggtggt
     9181 ctggtcgagg cgggcagcgt ccagcgccac ggcgccgacg tcagcttcgg cgtcaccgac
     9241 aacgccgcca ccacccgggt gatctttcgc ggcgacctac ccgacctgtt ccgcgaaggg
     9301 caggggatcg ttgctcaggg cgcgttccag cccgaccgca ccttcgtcgc ccatcaggtg
     9361 ctggccaagc acgacgagaa atacatgccg cgcgaggtcg ccgacaagct caaggccaag
     9421 ggcgagtggc gtcctgaggc atcagccgcc gcgaccggaa cctccacgct gtgatcagcg
     9481 aattcggcgc ctatgccctg atcctcgcct tggtcctcgc ggtgctgcag accctgctgg
     9541 ccgccgcggg tcgcattcgg cgctcgccgg tgctcgccgg agcggcgcaa ggcgcggccg
     9601 tcgcctgtgc aggcgtggtg gctctggcat tcctgacgct gatcttcgcc ttcgtgacgt
     9661 cggacttctc ggtcgccaac gtcgctaaca acagccacac cgacaagccg atgctctaca
     9721 aggtcgccgg cgcctggggc agccatgagg gctcgctggt cctttggtgt ctggtcatga
     9781 ccggtttcgg cgcggcgctg gcgagagccc cgagttcgac cggaggcctg ccgttcggcc
     9841 tcaagacctc ggcggtcgcc gtgcaaggcc tgttgggcag cctgttcctc gccttcgccg
     9901 tcttcacctc caaccccttc gcgcgtctcg atccggctcc ctttcagggc gcgtcgctga
     9961 acccgctgct gcaggatcca gcgctcgcct tccatccgcc gcttctctac gcaggctacg
    10021 tcggtttctc ggtgaccttc tcgctcgccg tcgccgcctt gatcgagggt cgcgtcgatc
    10081 cggcctgggg ccgctgggtc cggccatggg cgctggcgtc gtgggtgctg ctgaccgtcg
    10141 gcatcaccct cggctcgttc tgggcctatt acgaactagg ctggggcggc tggtggttct
    10201 gggatccggt cgagaacgcc agcttcatgc catggcttgc gggcgcggcc ttgctgcaca
    10261 gcgcggtcgt gaccgagcgg cgcggcgcgc tggcgggctg gaccgtgttc ctcgccctgc
    10321 tggccttcac tttttccatg ctcggcgcct tcctcgtccg ctccggcgtc ctgaccagcg
    10381 tccacgcgtt cgccgtcgat cccgagcgcg gcctgatgct gctgggcatc ctcggcgtca
    10441 cggcgggcgc ggccttcgcc ctgttcgcct ggcgcgcacc caaactgacc ggaggcggcc
    10501 tgttcgcccc gatcagtcgc gaaggcgcgc tggtgctcaa caacctgttc ctcacggcgg
    10561 cggcggcgac cgtcctgctg ggcaccctct atccgctgat cctgcaggcg gcgacgggcg
    10621 cgaccatctc ggtcggccca ccctatttcg ccctgacctt cacgccgtta atggtcgtgg
    10681 ctttcctgat cctgcccgct ggaccgctgc tcgcgtggaa gcgcggcgac ctcaaggggg
    10741 caggacagag gctcgccatc gccgccggtc tggccctgct gtgcgctctc gccggatggc
    10801 tggcgttcga gccgcgcaag gccatcgctg caggcgggat cgccatcggc acctggctga
    10861 tcctcggatc tctggccgag gtggtcgagc gggtgcggct gttccgcgtg ccgctggccg
    10921 aagtcggtcg ccgggccaag gccctgccgc tcggcgcatg gggcatgacg ctggcccata
    10981 ccggacttgg cgtgttcgtg ctcggcgccg tgatcgagac cggtttcaag gtcgaggcgg
    11041 ctgcagcgct gtcgctcggc ggaacggtca aggcaggcca ctattcggtg cggctcgatc
    11101 aggtccatat cgtcgaaggc cccaactatc tggccgagcg gggccatttg acggtgaccc
    11161 cggaccacgg cggacggccc gaggtcgtcg tcgccgagcg tcgcttcttc cccgccggtg
    11221 gtcagaccac gaccgaggtc ggcctcgact tccggggtct cgacgacgtt tatgtcgttc
    11281 tcggtgagcg ccgggcgaca ccacaagcgg ctcaggcgtg gacggtgcgc gtctactgga
    11341 atccgtgggc gcgactgatc ttcctcggcc ctctaatcat ggcgctgggc ggcgtcctgt
    11401 ccctgcttga ccggcgtctg cgcatcggtg tcgccggtcg ccgcaagacc gtcgagaccg
    11461 tcgagagcgt cgcgtgagac gcgccgcgct caccttcgca ttcgctcttc tcgccaccac
    11521 ggcgttggct gaacctgccg ctgctcccga ccgcccgctg acgtcgccgt cgcaggaagc
    11581 gcgcgcccag acgctgtttc aggacattcg ctgtgtcgtc tgtcagcacg aatccatcgc
    11641 cgacagtccg gccgagatcg ctgcggacat gcgccgtctg gtccgtgaag agatcgcctc
    11701 gggatcgagc gatcaggcca tccgcgccga cctcgtccgc cgctggggcg actacatcct
    11761 gtttaagccg ccggtgaaca tcgcgacctt gctgctctgg ttcggccctc tgctgttagt
    11821 cctgctggcc ggaggggctt tcatcgccct ctccttccgt ggaaagcccg tcgaaacacc
    11881 actttcgccc gacgaggagc gccggttggc cgaacttgtc catccggacc cgctccgccc
    11941 ctatcctgac gcaacttcgt cccacgactg acgttcgggg ggaacaatcc tcccattgcc
    12001 cggcttggtt tcggccccga cgggtctata tctgaatgcg aaacggcttc ccggtgaagc
    12061 cgaaagagga tcgctaaacg acgatgctga agcggaaaga gttcattctg ggggccgctg
    12121 caggcctgat cattgcggcc ggagccaccg ccggtggggt cgtgtcctgg cccagcgccc
    12181 aagccgaggg gcgtcccgga cagctgatgg cctcggccgg cgctgcgggc ctcgcatttg
    12241 ccccgccgca aggcgcgcca ctcagcttcg ccgacatcgt ccagcaggtc gccccggcgg
    12301 tcgtgcagat cgaggtcgag acccccgtgg aacgcccaca gggcatccaa ggttttcaga
    12361 ttccaggcct gcccggtatt gtaataccgg gcccgggatc gcagcaggcc gatc
//
LOCUS       DASVTM010000075         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_699694,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000075 DASVTM010000000
VERSION     DASVTM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699694"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..607
                     /gene="rbfA"
                     /locus_tag="VF633_02055"
     CDS             125..607
                     /gene="rbfA"
                     /locus_tag="VF633_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX8469870.1"
                     /translation="MSKLKPKSKSTAGLVSQRQLRASEMIRHALVEVMRENEIRDPAL
                     AGVSVTVTEVRLSPDLKHATCFVEPLGAGVATSDTANHIDEIVKALNAHAKFLRGALG
                     RHIDMRFTPDLRFRHDESFAAAERLNRLLDDPRVRADVFIPPVEEDEEPEVTSDPKES
                     "
     gene            607..1551
                     /gene="truB"
                     /locus_tag="VF633_02060"
     CDS             607..1551
                     /gene="truB"
                     /locus_tag="VF633_02060"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX8469871.1"
                     /translation="MARKKRGELVNGWVCLDKPFEMGSTEAVSKIRRLFNAQKAGHAG
                     TLDPLASGILPIALGEATKTVPFMMEAEKVYRFSINWGVSTDSIDREGEIIGRSDVRP
                     SVEAVRAALPTFVGEIDQTPPQFSAIKVDGQRAYDLAREGVEFELASRKVTIHEAEVT
                     DAPDAANPQQDWVEITIRTGKGVYVRSLARDLAVMLGAEGHVCALRRERVGPFSTENA
                     VTLDFLTDLVHRDAAFEGILPVATALDDIPELAITDQDAFSLRQGRPIVLLPRQVETL
                     KSHLRDGSRTVSAFQGQTLVALGQLRAGRLEPDRVFNL"
     gene            1632..1901
                     /gene="rpsO"
                     /locus_tag="VF633_02065"
     CDS             1632..1901
                     /gene="rpsO"
                     /locus_tag="VF633_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX8469872.1"
                     /translation="MSITAERKNEVIADNARTAGDTGSAEVQVAILSERIANLTEHFK
                     THKKDNHSRRGLLKMVSQRRRLLDHLTKVDKERYTALITKLGLRR"
     gene            2141..>3721
                     /gene="pnp"
                     /locus_tag="VF633_02070"
     CDS             2141..>3721
                     /gene="pnp"
                     /locus_tag="VF633_02070"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX8469873.1"
                     /translation="MFDIKRKTIDWAGRPLTLETGRIARQADGAVLATYGETVVLATV
                     VYGRSPKPGLDFFPLTVNYQEKTFAAGKIPGGYFKREGRPTEKETLVSRLIDRPIRPL
                     FVKGFKNETQVVITVLQHDMENDPDIVGMVATSAALTISGVPFQGPIGAARVGYIDGE
                     YVLNPTLDQLPDSQLDLVVAGTQDALMMVESEAKELSEEVMLNALMFAHRGMQPVIDA
                     IIELAEHAAKEPFDFTAEDHSDVVTSIQSLVGEDIKAAYTHVGKHDRHAAVGAAKKKA
                     SAALVKTEENPDGVDSAKFGTAFKECEAHVLRRDIIDNGHRVDGRALDKVRDIVSEVG
                     ILPRTHGSALFTRGETQAIVVATLGTGEDEQYVDALSGTYKEKFLLHYNFPPYSVGET
                     GRMGGAGRREIGHGKLAWRAIRPMLPKAEDFPYTIRIVSEITESNGSSSMASVCGASL
                     ALMDAGVPLTRPVSGIAMGLILEPSGEFAILSDILGDEDHLGDMDFKVAGTREGITSL
                     QMDIKVAGITEEIMRKAIA"
BASE COUNT          708 a         1307 c         1094 g          612 t
ORIGIN      
        1 agcctgacct gctgacctgc tgaaactgga catcacggtg cgaggcgact agaccgcccg
       61 acttcccttt tcggagggcg tatttccagt cgggtccgat ccccgacgcg tgaaggatca
      121 gctcgtgagc aaacttaagc cgaagtccaa gtccaccgcc ggcctcgtca gtcaacgtca
      181 gctgcgtgcg tcggaaatga tccgccacgc cctcgtcgag gtcatgcgcg agaacgagat
      241 ccgcgacccg gccctcgccg gcgtctcggt gacggtgacc gaagtccgcc tgtcgcccga
      301 cctcaagcac gcaacctgct tcgtcgagcc gctgggcgca ggcgtcgcca cctccgacac
      361 cgccaatcac atcgacgaga tcgtcaaggc gctcaacgcc cacgccaaat tcctgcgcgg
      421 cgcgctgggc cgtcacatcg acatgcgctt cacccccgat ctgcgcttcc gccacgacga
      481 gagcttcgcc gccgccgaac gcctgaaccg cctgctcgat gacccccgcg tccgcgccga
      541 cgtcttcatt ccgcccgtcg aggaagacga agagcccgaa gtcacgtcag acccgaaaga
      601 gtcctgatgg cgcgcaagaa gcgcggcgaa ctggtcaacg gctgggtctg cctcgacaag
      661 ccgttcgaga tgggttcgac cgaggccgtc tcgaagatcc gccgcctgtt caacgcccag
      721 aaggccgggc acgccgggac gctggatccg ctcgcctcgg gcattttgcc gatcgcgctc
      781 ggcgaggcga ccaagaccgt ccccttcatg atggaggcgg agaaggtcta tcgcttctcc
      841 atcaactggg gcgtctcgac cgacagcatc gaccgcgagg gcgagatcat cggtcgttcg
      901 gacgttcggc cctcggtcga ggcggttcgc gcggcgctgc cgaccttcgt cggcgagatc
      961 gatcagacgc cgcctcagtt cagcgccatc aaggtcgacg gacagcgcgc ctatgatctg
     1021 gcgcgcgagg gcgtcgagtt cgagttggcc agccgcaagg tcaccatcca tgaggccgag
     1081 gtcaccgacg cgcccgacgc cgccaatcct caacaagact gggtcgagat caccatccgc
     1141 accggcaagg gcgtctatgt ccgctcgctg gcccgcgatc tggcggtgat gctgggcgcc
     1201 gaggggcatg tctgcgccct tcggcgcgag cgggtggggc cattcagtac cgagaacgcc
     1261 gtcactctgg atttcctgac cgatctggtg catagggacg ccgcctttga aggcattctg
     1321 cccgtagcga ccgccctgga cgacatcccg gagctggcca ttacggatca ggacgccttc
     1381 tcgcttcgtc agggacgccc gatcgttctg ctcccccgac aggtcgagac gctcaagagc
     1441 caccttcggg atggctcgcg gaccgtttca gcctttcagg gtcagactct cgtcgctctc
     1501 ggtcagttgc gggccggccg gctagaaccc gaccgcgtat ttaacctcta ggtccgagcc
     1561 atctggcgac gaccgggacc ccacggcctc aagtagcgcg caagcgcgat aggccaagaa
     1621 aggactctac gatgtcgatt actgctgaac gcaagaacga agtcattgcc gacaacgccc
     1681 gcaccgctgg cgacaccggc tcggccgagg tccaggtcgc gatcctttcg gaacgcatcg
     1741 ccaacctcac cgaacacttc aagacccaca agaaggacaa ccactcgcgt cgcggcctgc
     1801 tcaagatggt ctcgcagcgt cgtcgccttc ttgaccacct gaccaaggtc gacaaggagc
     1861 gttacaccgc tctgatcacc aagctgggcc tgcgccgcta agcgctgtcc acgacaacgg
     1921 tttcgaggcg cgggccacgt ccgcgcctct ttccgtacct aaagaccagc cgcgcgccgc
     1981 ggtacgtacg gcgcagacgc gagggccaga ccggtcctct tctcccggac ttatcgcgat
     2041 ggcgcgatct gtccatggac cgaaggactg aacgcccgac gctccaccca ttgatgggac
     2101 cccgcgcgga atacgccgcc cgggatacga aagacgaaac atgttcgata tcaaacgcaa
     2161 gacgatcgac tgggccggcc gcccgctgac gctggaaacg ggccgcatcg cccgtcaggc
     2221 tgacggcgcc gttctggcca cctacggtga gaccgtcgtt ctcgccaccg tcgtctacgg
     2281 tcgctcgccc aagccgggcc tggatttctt cccgctgacc gtcaactacc aggaaaaaac
     2341 cttcgccgcc ggcaagatcc cgggtggcta tttcaagcgt gaaggccgtc cgaccgagaa
     2401 ggaaaccctg gtttcccgcc tgatcgaccg tccgatccgt cccctgttcg tcaagggctt
     2461 caagaacgag acacaggtcg tcatcaccgt gctgcagcac gacatggaga acgaccccga
     2521 catcgtcggc atggtcgcca cctccgccgc cctgacgatc tcgggcgtcc cgttccaggg
     2581 cccgatcggc gcggctcgcg tcggctacat cgacggcgag tacgtcctga acccaaccct
     2641 cgaccaactg cctgactcgc aactcgacct cgtcgtcgcc ggcacgcaag acgccctgat
     2701 gatggtcgaa tccgaagcca aggagctgtc ggaagaagtc atgctgaacg ccctgatgtt
     2761 cgcgcatcgc ggcatgcagc cggtgatcga tgcgatcatc gaactggccg agcacgccgc
     2821 caaggagccc ttcgacttca ccgccgaaga ccactccgac gtcgtcacct cgatccagtc
     2881 gctggtcggc gaagacatca aggccgccta cacccacgtc ggcaagcatg accgtcacgc
     2941 cgccgtcggc gccgccaaga agaaggcctc ggccgctctg gtgaagaccg aagaaaaccc
     3001 ggacggcgtc gattccgcca agttcggcac ggccttcaag gaatgcgaag cccacgtcct
     3061 gcgtcgcgac atcatcgaca acggccaccg cgtcgatggc cgcgccctcg acaaggtccg
     3121 cgacatcgtc tcggaagtcg gcatcctgcc ccgcacccac ggctcggccc tgttcacccg
     3181 cggtgaaacg caagccatcg tcgtcgccac gctcggcacc ggcgaagacg agcaatacgt
     3241 cgacgccctg tccggcacgt acaaggagaa gttccttctc cactacaact tccctccata
     3301 ctcggtcggt gaaaccggtc gtatgggcgg cgctggtcgt cgtgaaatcg gccacggcaa
     3361 gctcgcctgg cgtgcgatcc gtccgatgct gccgaaggct gaagacttcc cgtacaccat
     3421 ccgcatcgtg tccgagatca ccgagtccaa cggctcgtcc tcgatggctt cggtctgcgg
     3481 cgcctcgctg gcgctgatgg atgcaggcgt gcctctgacc cgtccggtct ccggcatcgc
     3541 catgggcctg atcctcgagc catccggcga gttcgccatt ctgtccgaca tcctgggtga
     3601 tgaagatcac ctcggcgaca tggacttcaa ggtggccggc acgcgcgagg gcatcacgtc
     3661 cctgcagatg gacatcaagg tcgcgggcat caccgaagag atcatgcgca aggcgatcgc
     3721 c
//
LOCUS       DASVTM010000076         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_166937,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000076 DASVTM010000000
VERSION     DASVTM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166937"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VF633_02075"
     CDS             <1..419
                     /locus_tag="VF633_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX8469874.1"
                     /translation="VALAILMPIVSAIGGNAGTQALTVTVRALATRELNSSNAPRTFV
                     RELVVGLANGLILAPLIGIATALWFRNEDWRIGLVIGAAMVLNLIVAATFGVLTPITL
                     SKLKFDPAVSSAVFVTATTDFFGFLIFLGLATIVLL"
     gene            590..2104
                     /locus_tag="VF633_02080"
     CDS             590..2104
                     /locus_tag="VF633_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEX8469875.1"
                     /translation="MQRLKISREGVILIKSFEGFRPRAIRRDNGQWVIGYGHTASARE
                     GASVSETDAELLLQYDLIPIVKALNEGVAARLNQHQFDALASFAYSVGLDRFQGSDVL
                     ARLNAGFAGEAADALVGWPEPVLPETSLRRRAAERALFVADPASPVALSDLLVAPIPA
                     LTVAEKSGTEADATLAEVMGDPAPQAVAPLSETNPVLRQAEALRAPPSPATDHDEPAL
                     APAAFTSSAVVFNYPGAQAMQRYSAYPGSIVGPLPAPLHATRAVSEVQPIQSTEADVE
                     SVSSASDTTAAISAPEPAQPVETDVEAAATPEAPVVPATDLSAPWGTPPVPRPFPALA
                     PESEVLFPDFSAVPTAAPTGSPNPWPSISAVSEPRTDGELIVTSTEGGAVFASPRLVW
                     PHEDQTSGEALPMFEDDGSLRLAPGNIIRHEPLQQTAPTSIPWGELGAYGLMGAFGLV
                     SFGMSMAAFRRASQDNPGGDGTTLIAIVLAVIGATCVGVSAYNLYRRWGRTDDE"
     gene            2184..2372
                     /locus_tag="VF633_02085"
     CDS             2184..2372
                     /locus_tag="VF633_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEX8469876.1"
                     /translation="MPAKSAAQQKAAGAALSAKRGDTPKSELKGASRQMVDSMTEKQL
                     EDFAHTKRKGKPEHVKSN"
     gene            2487..>2716
                     /locus_tag="VF633_02090"
     CDS             2487..>2716
                     /locus_tag="VF633_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX8469877.1"
                     /translation="MSLVPAPPPPHRTFSQVLEDLGVGDASLTLDEVVEAFGDRGIGA
                     LILVLSLMALIPWPPGGKAIFAAPIILLAVELA"
BASE COUNT          432 a          970 c          809 g          505 t
ORIGIN      
        1 tcgtggcgct ggccatcctg atgccgatcg tctcggccat cggcggcaat gccggcaccc
       61 aggccctgac cgtcaccgtc cgagccctcg ccacgcgtga gctcaacagc tccaacgctc
      121 ctcgtacgtt tgtacgcgaa ctcgtcgtag gtctcgccaa cggcctgatc ctggcgcccc
      181 tgatcggtat cgccactgcc ctgtggttcc gtaacgagga ctggcgcatc ggcctcgtca
      241 tcggagccgc catggtgctg aacctgatcg tggccgcgac cttcggcgtc ctgaccccga
      301 tcaccctgtc caagctgaag ttcgacccgg ccgtgtcgtc cgccgtgttc gtcacagcga
      361 ccacggactt cttcggcttc ctgatcttcc tcggactggc gacgatcgtc cttctttaag
      421 gatctttagg ccggttctcc ccgcataccg gtgtccctgc gtctcctggg agtggaatcc
      481 ggtttcagac ggcgccgcac tgcggcatga tccggcgtga gccttgctgg tgctcgctca
      541 aggcgaactc gcctgtctcg atttgggtca ttccgtgtct gaacccgtca tgcaacgtct
      601 gaagatttcc cgcgagggcg tcattctgat caagagcttc gagggctttc gcccgcgggc
      661 aatccgtcgc gacaacggac aatgggtgat cgggtacggc cacaccgcgt ccgcacgcga
      721 aggcgcttcg gtcagcgaga ccgacgccga actgctgctc caatacgacc tcataccgat
      781 cgtcaaggcg ctcaacgagg gagtcgcggc acgcctcaac cagcatcagt tcgacgctct
      841 tgccagtttc gcctactccg tgggcctcga tcgtttccag ggctccgacg tgctggcgcg
      901 cctgaacgcc ggttttgcgg gcgaggcggc ggacgccctt gtgggctggc ccgagccggt
      961 tctgcctgag acgtcactac gccgacgcgc cgccgagcgc gccctgttcg tcgccgaccc
     1021 cgcctcgccg gttgccctgt ccgaccttct ggtcgccccg atcccggcgc tcaccgttgc
     1081 cgagaagtcg ggcacggagg ctgacgccac gttggcggag gtgatgggcg atccagcacc
     1141 gcaggccgtc gccccactct cggaaacgaa cccggtcctg cggcaagcgg aagccctgcg
     1201 agccccccct tcgcccgcga cagaccacga cgaacctgcc cttgctcccg cagccttcac
     1261 gtcctccgcc gtggttttta actacccggg cgcccaggcg atgcagcggt attcagccta
     1321 tcccggctca atcgtcggcc ccctgcccgc gcccctccac gccacccgtg cggtcagtga
     1381 ggtgcaaccg atccaaagca ccgaggccga cgtcgaatct gtatcttctg cttcagacac
     1441 gaccgccgct atctccgctc ccgaacccgc tcaaccggtc gaaacggacg ttgaagcggc
     1501 agcaacacct gaagccccgg tcgttccggc gacggatctc tcggcgccgt gggggacgcc
     1561 gccggtcccg cgccccttcc ccgccttggc gccagaatcg gaagtgctgt tccccgactt
     1621 ctcagccgta ccgacggctg cgccgaccgg ctccccgaat ccatggccct cgatcagcgc
     1681 cgtcagcgag cctcgcaccg acggtgaact gattgtgacc tcgactgaag gcggggccgt
     1741 ctttgcctcg ccgcgactgg tctggccgca cgaagatcag acatccggtg aggccctgcc
     1801 gatgttcgag gacgatggct cgctgcgcct cgcgcccggc aatatcatcc gtcacgagcc
     1861 tctccagcaa accgcgccga ccagcatccc gtggggcgag ctcggcgcct acggccttat
     1921 gggcgcattc ggactggtgt cgttcggcat gtcgatggcc gcattcaggc gtgcctccca
     1981 ggataatccc ggcggggacg gcacgacctt gatcgccatc gtactcgccg tcatcggcgc
     2041 gacctgcgtc ggagtctcgg cgtacaatct ctaccgtcgc tggggccgca cggacgacga
     2101 gtaaggcccg tcggggccgc cgcgactgca ggaacgcccg gccgcgctgg ccgttgaaat
     2161 gtcctcagag aggagatttc gtcatgcccg ccaaatccgc cgcccagcaa aaagccgccg
     2221 gcgccgcatt gtcggcaaag cgcggcgaca cgccgaaatc cgagctcaag ggcgcttcca
     2281 gacagatggt cgactcgatg accgagaagc agctagagga ttttgcccac acgaaacgca
     2341 agggcaaacc cgagcacgtc aagtccaact gaggcgcccg ggttttcatc ctgaaatcgt
     2401 cgtaacactc tgcgacagtg ctggcgtggg gcccccgccc cgcttacatc acggccgggc
     2461 cgcaatgggc cgttgaacgg tatccgatgt ctctggtccc cgcccctcct ccgcctcacc
     2521 gcaccttctc gcaggtgctg gaggacttgg gtgtaggcga cgcgagtctc actttggatg
     2581 aagtggtcga ggcgttcggt gatcgaggta ttggcgcgct gatccttgtg ctgtcgctca
     2641 tggccctgat cccttggcct cccggcggca aggctatctt tgctgccccg atcatcctgc
     2701 ttgcagtcga gttggc
//
LOCUS       DASVTM010000077         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_752488,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000077 DASVTM010000000
VERSION     DASVTM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752488"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VF633_02095"
     CDS             complement(<1..267)
                     /locus_tag="VF633_02095"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEX8469878.1"
                     /translation="MSPSTDHAVSEIEGRARFDQIRYAQVWEDADVLVAALRVQPGDT
                     VVSIASAGDNAIALLAQGAERVIAADLNPTQLVCVRLRVAAYRTL"
     gene            complement(264..>1929)
                     /locus_tag="VF633_02100"
     CDS             complement(264..>1929)
                     /locus_tag="VF633_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX8469879.1"
                     /translation="SPFTFTFARRVYGHVYRAFSAMMGVSPEAIADQRATFDTMLGRV
                     HGRVYYNLLAWYRALSLFPGFKANRAFMEQMMGVGEPLPAALVHRIAPDDHSGLDRLL
                     DRLKLVGVLARLVVYNFLLPGVINRFYSRLNLALAESDADIEGLSATQLAARYRMLET
                     RLLERWDAPLINDFLCMIAFGASRKVMQTWAGDEGLTLHSDIMIGQGDIVSAEPARRI
                     RAMGDVVRGDAVLIERLAVGDVTAVRTHVRLARLFDDYIARFGDRCTEELKLESVTLH
                     EDPRQLLSAVAAAARAEPRADRPANLAEATDLRLRSLFAGRPIKRRLAGALTAWAKTR
                     VRDRENLRFERTRLFGRVRRIVRASGRRLTEAGLLDAENDIFLLTVEEWLGAVEGGVP
                     DRGLKALVELRRAEHAADMARADPPERLTIRGGFLIGLSSAEAAAVVDDEDPSSLVRT
                     GQPCCAGTVTARVRVVADPRVEALEAGEILVARHTDPGWIAVFANASGVIAERGSLLS
                     HSAIVAREMGVPCVVALKNVTSWLKTGDLVRLDGGTGRVEKLESAS"
BASE COUNT          354 a          650 c          629 g          296 t
ORIGIN      
        1 cagggtccgg taggccgcga cccgcaaacg cacgcagacg agctgcgtcg ggttcaggtc
       61 cgccgcgatg actcgctccg caccctgcgc gaggagggcg atggcgttgt caccggccga
      121 cgcgatggag acgacggtgt ctccgggctg gacgcgaagg gcggcgacca gcacatcggc
      181 atcctcccag acctgggcat agcggatctg gtcaaaacgc gcccggccct cgatctcgga
      241 gacggcgtgg tcggtgctcg ggctcatgat gcggattcca gcttctcgac ccgacccgtg
      301 ccgccgtcca gtcgcaccag atcgccggtc ttgagccatg acgtgacgtt cttcagcgcg
      361 accacgcagg gcacgcccat ctcgcgagcc acgatggcag aatgggacag caggctgccc
      421 cgttcggcga taacgccaga ggcgttggcg aagacggcga tccagcccgg atcggtatga
      481 cgggcgacca ggatttcgcc cgcctccagt gcctctacac gcggatcggc gacgacacgc
      541 acgcgggctg tgacggtccc ggcgcagcag ggctggccgg tccgcacgag gcttgacggg
      601 tcctcatcgt caaccacggc cgccgcttct gcgctggaca aaccgatcag aaaaccgccg
      661 cgaatggtca gtcgctcggg cgggtcggca cgcgccatat cggcagcgtg ttcggctctc
      721 cgcagctcga ccaaagcctt caggccgcga tccgggacac cgccctcgac ggcgcccagc
      781 cattcctcga cggtcagcag gaagatgtcg ttctctgcat caagcaagcc ggcttcggta
      841 agccgacgac ctgacgctct gacgatccgg cggacccgtc caaacagccg cgtccgctcg
      901 aaccggagat tctcccgatc ccgcacgcga gtcttggccc aggctgtgag agcccccgcg
      961 agccgtcgct tgatcgggcg tccggcgaac aggctgcgca accgcaagtc ggtcgcctca
     1021 gcgagattgg cggggcggtc ggcgcgcggc tcggctcgag cagccgctgc tacggcggac
     1081 aacaactgac gcgggtcctc gtgaagcgtg acgctctcca gcttgagctc ctcggtgcag
     1141 cgatcgccga aacgagcgat ataatcgtcg aacaaccggg ccaatcggac gtgcgtccgg
     1201 acggcggtga cgtcaccgac ggccagtcgc tcgatcagga cggcgtcgcc acggaccacg
     1261 tcgcccatcg cgcgaatgcg tcgcgccggt tcggccgaga cgatgtcgcc ctggccgatc
     1321 atgatgtcgg aatgcagcgt caagccttcg tcgccggccc acgtctgcat caccttccgc
     1381 gacgccccaa aggcgatcat gcacaggaaa tcgttgatca gtggcgcgtc ccaccgctcc
     1441 agcagacggg tctcaagcat ccggtagcgg gcagccagct gggtcgccga caagccttcg
     1501 atgtccgcgt cgctctcggc cagcgccaga ttcaggcggc tgtagaagcg gttgatgacg
     1561 cccggcagaa ggaagttgta gaccaccaga cgcgcgagta ctccgaccag cttcagccta
     1621 tccagcagtc gatccagacc tgaatgatcg tcaggcgcga tcctgtgtac cagagcagcg
     1681 ggcaggggtt ctccgacccc catcatttgc tccatgaagg cgcggttagc cttgaagccc
     1741 gggaacagcg acagcgcgcg gtaccaggcc agcaggttgt aatagacccg accgtgtacc
     1801 cgccccagca tggtgtcgaa cgttgcgcgc tggtcggcga tggcttctgg actgacgccc
     1861 atcatggcgc tgaacgcgcg atagacgtgg ccgtagacgc gacgtgcgaa ggtgaaggtg
     1921 aaagggctg
//
LOCUS       DASVTM010000078         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_806192,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000078 DASVTM010000000
VERSION     DASVTM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806192"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VF633_02105"
     CDS             complement(<1..558)
                     /locus_tag="VF633_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469880.1"
                     /translation="MNYKSGLGLLTSLLLASTAMPAFAQTQTISAPETGPEEQATGLE
                     DIVVTATKRETNLQDTPISISVVNAEALADRHIQSLMDLADGAVPSLRVATFEARQSA
                     LTIGIRGIVPNDANQPAREQGVGVYVDGVYLARQHGLGAALLDLERIEVLKGPQGTLF
                     GRNTEGGALSMVTKKPTGEFGMRAVA"
     gene            867..1499
                     /locus_tag="VF633_02110"
     CDS             867..1499
                     /locus_tag="VF633_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8469881.1"
                     /translation="MPSNTINKLGHRIGARGGRTRASILAATRRLLDSRHLGEIRVAD
                     VAAEAGLSPSNFYTYFKTVEEPVLALCEASAADFQVLARHLDGDWSGEQAFLAARAYV
                     VDTLAFWQAHGPVLRIEHMLADKGEAGFIETRVKRLRRVHLAFERRIAQAHATGYHAK
                     GLDPRLTSYQVANLVESMADGFELLKRGDTPPDTIIDTTAYIVVKIVTGR"
     gene            complement(1496..2161)
                     /locus_tag="VF633_02115"
     CDS             complement(1496..2161)
                     /locus_tag="VF633_02115"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX8469882.1"
                     /translation="MPLPVLLAILLGLSGLAVGQALAVVSDRLPGSEDAVAATKSWSS
                     LATSRRHLLFSFAAAGIGVWSALAANWPVAVAGAVLGWQLLVIADVDAEHFWLPDVLT
                     LPLIGSGLMANGLLTHGLPWSQAIGAVGGFGLLWGLSWLYRTVRKRDGLGGGDPILFA
                     GAGAWVGWTGLPAVFLTACAVLLFGVLLRLLWRKPVRGADRAPFGTLLAIGVWLVWLY
                     GGV"
     gene            complement(2201..>2875)
                     /locus_tag="VF633_02120"
     CDS             complement(2201..>2875)
                     /locus_tag="VF633_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX8469883.1"
                     /translation="MFGLYAAGIVSALLVSFVIRKVFWRGAVEPFMMELPTYKMPDLK
                     SVGFNLWVRARIFMERAARIILPLMVLIWVLSTFPYPPEGATGPAIDYSFAGMVGHAL
                     EPLFRPIGFNWQMVIALVPGMAAREVAVAALGTVYAIGDVESSTTALSATLMTQWSLA
                     TGLSFLAWYIFAPQCVATLGVVKRETNSWKWMWVMVGYMFGLAYLAAFATYHIAVALG
                     GGTIGS"
BASE COUNT          570 a          934 c          911 g          460 t
ORIGIN      
        1 ggcgacggcg cgcatgccga actcgccagt aggcttcttg gtgaccatcg aaagcgcgcc
       61 gccttcggtg ttgcggccga acagggtgcc ttgcggtccc ttcagaactt cgatgcgctc
      121 gaggtcgagc agagcggcgc cgaggccgtg ctgacgagcc agataaacgc cgtcgacata
      181 gacgccgacg ccttgttcac gggcgggctg gttggcgtcg ttcggcacga tgccgcggat
      241 gccgatggtc agggccgact ggcgggcttc gaaggtggcg acgcgcagcg atgggacggc
      301 gccgtcggcg agatccatca ggctctgaat gtggcggtcg gccagagctt cggcgttgac
      361 gaccgagatc gaaatcgggg tgtcttgcag gttggtctca cgcttggtgg cggtgaccac
      421 gatgtcttcc aggcccgtgg cctgttcttc cgggccggtt tccggcgctg agatggtttg
      481 cgtctgtgcg aaagcgggca tcgccgtgct ggcgagcaaa aggctggtaa gcaggccgag
      541 gccggatttg tagttcatct tgagtcccct ttgttgcggg gacgtatagg tgactaatat
      601 gtagcctaag ccgcaggaat atgacggctc ggtttcggaa ttacatcgat agcgacatgc
      661 gtttgttgca aatctgtcag atatacacag tcgttcgtca cgatacggat catcttttac
      721 aataacgaca ttccagacaa accggaatcg ctcccggccc tccgagcggc cagctgccaa
      781 ctgcgttgac gcgacagcgc aattctggcc gataggttgg agcggcgacg cctcccctcc
      841 ctccgctttc aagactcgcg ttcctcatgc catcaaacac catcaacaag ctgggacacc
      901 ggatcggcgc gcgcggcgga cgcacgcgcg cctcgatcct cgctgcgacg cgcaggcttc
      961 tcgacagccg tcatctgggt gagattcgcg tggccgacgt cgcggccgag gcagggctgt
     1021 cgccctcgaa cttctacacc tacttcaaga ccgtggaaga gcccgtgctg gctctgtgcg
     1081 aagccagcgc cgccgacttc caggtgctcg cgaggcatct ggacggcgac tggtcgggcg
     1141 agcaggcttt tctggcggcg cgggcgtacg tcgtggatac gctggcgttc tggcaggccc
     1201 atgggccggt gctgcgcatc gagcacatgc tggcggacaa gggcgaagca ggcttcatcg
     1261 aaacccgggt caaacgtctg cgtcgtgtcc acctggcgtt cgagcgtcgc atcgctcagg
     1321 cccacgccac cggctatcac gccaagggac tggacccgcg cctgacgtcg tatcaggtcg
     1381 ccaatctggt ggagtcgatg gccgacggat ttgagctgct caaacgcgga gacaccccgc
     1441 cggacaccat catcgacacg acggcctaca tcgtcgtcaa gatcgtgacc ggccgctaga
     1501 cgccgccgta cagccagacc agccagacgc cgatcgcaag cagggtgccg aacggcgcac
     1561 ggtcggcgcc tctcaccggc ttgcgccaca acagtctgag cagaacgccg aacagcagaa
     1621 cggcgcatgc cgtcagaaac actgctggaa gaccggtcca gccaacccac gcccctgccc
     1681 ccgcgaacag gatcgggtca ccgccgccga ggccgtctcg cttacgaacc gttcggtaga
     1741 gccaagacag gccccacagc agaccgaaac ccccgaccgc gccgatcgcc tgagaccagg
     1801 gcagaccatg ggtcagcagg ccgttggcca tcagaccgct gcctatcagc ggcagggtca
     1861 ggacgtccgg cagccagaaa tgctcggcgt cgacatccgc gatcaccagc aactgccagc
     1921 ccagcacagc gcccgcgacg gcgacaggcc agttcgcagc tagcgccgac caaacgccga
     1981 tcccggcggc cgcaaacgaa aacagcaggt ggcggcgtga cgtagcgagg ctggaccagg
     2041 atttcgtcgc tgctaccgca tcttcactgc ccggcagccg atcgctgacg acggcgagcg
     2101 cctgacctac ggccagtcct gacaggccca gcaatatcgc caagaggacg ggaagcggca
     2161 tggacgaatc cgacgccgcg tcctgtgcgg cccctcggga ttagctgccg atcgttccac
     2221 cgccaagcgc aacggcgata tgataggtgg cgaacgccgc cagataggcg aggccgaaca
     2281 tatagccgac catcacccac atccatttcc agctgttggt ctcgcgtttg acgacgccga
     2341 gcgtcgccac gcactgcggc gcaaagatgt accaggcgag gaagctgagc cctgtggcca
     2401 gcgaccattg cgtcattagc gtcgcgctaa gggccgtggt cgagctttcc acgtccccga
     2461 tagcataaac ggtgccgagc gcggcgacgg ccacttcacg cgccgccatc ccgggcacca
     2521 gcgcgatgac catctgccag ttgaagccga tcgggcggaa caggggttca agggcgtggc
     2581 cgaccatacc cgcaaaggaa tagtcgatgg ccggcccggt cgcgccctcg ggaggatagg
     2641 ggaaggtcga caaaacccag atcagcacca tcaacggcag gatgatccgc gccgcccgct
     2701 ccatgaagat gcgcgcccgg acccacaggt tgaagccgac gctcttgagg tcgggcatct
     2761 tgtaggtcgg cagctccatc atgaaaggct cgaccgcgcc gcgccagaac accttgcgga
     2821 ttacgaacga caccagcagc gcactgacga tcccggcggc gtagaggccg aacat
//
LOCUS       DASVTM010000079         4508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_592095,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000079 DASVTM010000000
VERSION     DASVTM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592095"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2044)
                     /locus_tag="VF633_02125"
     CDS             complement(<1..2044)
                     /locus_tag="VF633_02125"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX8469884.1"
                     /translation="MLNKAYVYAMKMHGSQLRASGDPYFAHPIQVAGILTDYKLDTAT
                     IVTALLHDVVEDTSATRDDIAAMFGEEVAVLVEGVTKLSRLELQAEHTRQAENLRKFI
                     LAISKDVRVLLVKLADRLHNMRTLKFVKPEKRERISRETLEVYAPLGRSIGIHSIASE
                     LEELAFEHINPTARTAIQRRLEALKLEHGQAIEGVSHEVERVLSEAGITGANVFGREK
                     TPYSIWRKLQRKTVGFSSLSDIYGFRVIVPFEDDCYRALGVIHRAWPMVPERFKDFIS
                     TPKSNNYRSLHTTVVGPGGLRIEMQIRTEAMDRVAEDGVAAHWAYKNHSYGFDKEGMR
                     AEGGRDPLQNLRHLVQVIEHGEGGEDWVEYAKLEMYLDQVFVFTPKGRLISLPRGGMA
                     LDFAYGVHTEVGDTAAGVKINGELKPMRTPLQNGDVVEIIRGNKREAPTDWRSLTVTG
                     RARSAIRRHIRGAEREEFLRLGRATLEQTISKAGKSIDDVSLKPVLEVLAIANEADLF
                     EAIGRGRVSPVKVAETLFPALMGKIKPAQQRKSIQDARARLFVRGGGLTPGVSIHFGQ
                     CCTPLPGDRIVGILEKDKGLTVHTIDCQKLADFADDDSVWQDLQWTPQAEQGALGIAR
                     LRATVRNGVGVMGQVTTLIGEAGGNIRNLALAHRHQDFFDLNFDVEVEDAKHATMIM"
     gene            complement(2242..2601)
                     /gene="rpoZ"
                     /locus_tag="VF633_02130"
     CDS             complement(2242..2601)
                     /gene="rpoZ"
                     /locus_tag="VF633_02130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX8469885.1"
                     /translation="MARVTVEDCIQKVPNRFGLVLLAGNRARAIANGAALTLDRDNDK
                     NPVVALREIADETVMPAELLETVIVALQRVDERTEAEDEAEIVGLLAEPQHMNMAEQE
                     LIRALQSDRDGGQEERY"
     gene            complement(2693..3259)
                     /gene="folK"
                     /locus_tag="VF633_02135"
     CDS             complement(2693..3259)
                     /gene="folK"
                     /locus_tag="VF633_02135"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX8469886.1"
                     /translation="MQGSGVISNPAVNGLTVNIDGDLDLDAAVIVALGCNDKGVWSSC
                     RDALEAALTRFRSEGIDVVARSSWWSSQAWPDPTDPPFLNGVVIVRTTLDPHALMGAL
                     GRIEEMFGRRRSTQNAPRTLDLDLIAYGRERGDCDGLILPHPRAADRLFVMGPVAEIA
                     PDWRHPGGGFASALALTATVGTDARIAS"
     gene            3259..3828
                     /locus_tag="VF633_02140"
     CDS             3259..3828
                     /locus_tag="VF633_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX8469887.1"
                     /translation="MYPTARIALFIDGANLYSAARALNVDLDFRKLVESFREKAVLVR
                     AYYYTAVIEGEEFSPIRPLVDWLGYNGFSVVTKPVKRFTDAQGHSKLKGNMDIEIAVD
                     MIEMAPHLDEMVLFSGDGDFRRLVQAVQAKGVRVTVVSTTRSQPPMIADELRRQADDY
                     IDLADRLGDWGRPRTSTPSPSVRTFGDEV"
     gene            complement(3825..4142)
                     /locus_tag="VF633_02145"
     CDS             complement(3825..4142)
                     /locus_tag="VF633_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469888.1"
                     /translation="MFASPASADIDSVFGNTVVSHYPDGGWVKHWFNRDGVYSAQFSD
                     GRRITASWRVEGQKICLNGIRPSFMMISRFCSPLIEAGVGQRWASRDPLGRRVQNELI
                     PGR"
     gene            4418..>4508
                     /locus_tag="VF633_02150"
     CDS             4418..>4508
                     /locus_tag="VF633_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469889.1"
                     /translation="MTEFANLGLSPTTLQAVSDTGYTIATPIQA"
BASE COUNT          810 a         1420 c         1452 g          826 t
ORIGIN      
        1 ccatgatcat ggtggcgtgt ttcgcgtcct cgacctcgac gtcgaaattg aggtcgaaga
       61 agtcctggtg ccggtgggcc agggcgaggt tgcggatgtt gccgccggcc tcgccgatca
      121 gggtggtgac ctgacccatg acgccgaccc cgttgcggac cgtggcgcga aggcgggcga
      181 tgccgagcgc gccctgctct gcttgcggcg tccattgcag atcctgccag acgctgtcgt
      241 cgtcggcgaa gtcggccagt ttctgacagt caatggtgtg gacggtgagg cccttgtcct
      301 tttccagaat accgacgatc cggtcgccgg gcaaaggcgt acagcactga ccgaaatgga
      361 tgctgacgcc gggcgtaagg ccgccgccgc gcacgaacag ccgagcgcga gcgtcctgaa
      421 tggatttgcg ctgctgggcc ggtttgatct tgcccatcag ggccgggaac agggtctcgg
      481 cgaccttcac cggcgatacc cggccacggc caatcgcctc gaacaggtcg gcttcgttgg
      541 cgatggccaa tacttcgagg accggcttca gcgacacgtc gtcgatcgac ttgcctgcct
      601 tcgaaatggt ctgttccagc gtcgcacggc ccagacgcag gaactcctcg cgctcggcgc
      661 cgcggatgtg acgacggatc gcagagcgcg cgcgaccggt gacggtcagg ctgcgccagt
      721 cggtaggggc ctcacgctta ttgccgcgaa tgatctcgac cacgtcgccg ttctggagcg
      781 gcgtacgcat cggcttcagt tcgccgttga tcttgacgcc cgcagccgtg tcgccgacct
      841 cggtatgaac gccataggcg aagtcgagcg ccatgccgcc gcgcggcagg ctgatcaggc
      901 gccccttcgg tgtgaagacg aagacctgat cgaggtacat ctcgagcttg gcgtactcga
      961 cccagtcctc gccgccctcg ccgtgctcga tgacctgaac aaggtggcgc aggttctgca
     1021 gcggatcgcg cccgccctcg gcccgcatgc cttccttgtc gaacccatag gagtggttct
     1081 tgtaggccca gtgggcggcg acaccatcct cagccacacg atccatcgcc tcggtgcgga
     1141 tctgcatctc gatccgcagc ccgccgggac cgacgaccgt agtatgcagc gagcgatagt
     1201 tgttcgactt gggcgtcgag atgaagtcct tgaaccgctc gggcaccatc ggccaggcgc
     1261 gatggatgac gcccagcgcg cggtagcaat catcctcgaa cggcacgatg acgcggaagc
     1321 cgtagatgtc ggacagggac gagaagccga cggtcttgcg ctgcagcttg cgccagatcg
     1381 aatacggcgt cttctcgcgg ccgaagacgt tagcgccggt gatgccggcc tcgctcagaa
     1441 cccgctcgac ctcgtgcgag acgccctcga tggcctgacc gtgttcgagc ttgagggctt
     1501 cgaggcggcg ttggatggcg gtgcgggccg tgggattgat atgctcgaac gcgagctctt
     1561 ccagctctga cgcgatcgag tggatgccga tggaacgacc cagcggcgca tagacctcga
     1621 gcgtttcacg gctgattcgt tcgcgcttct cgggcttgac gaacttgagc gtccgcatgt
     1681 tgtgcaaacg gtcggcgagc ttgaccaaca ggacgcggac gtccttggag atggcgagga
     1741 tgaatttgcg caggttctcg gcctgacgcg tgtgttcggc ctgaagctcc agccgcgaca
     1801 gcttggtcac tccctccacc agaaccgcga cctcttcccc gaacatggcg gcgatgtcgt
     1861 cgcgcgtcgc cgaggtgtcc tcgactacgt catgcaacag ggcggtgacg atagtggcgg
     1921 tgtcgagctt gtagtcggtg aggatgcccg cgacctggat cgggtgggcg aaataggggt
     1981 cgccggaggc ccgcaactgc gagccgtgca tcttcatcgc atagacgtag gccttgttga
     2041 gcatcgcctc gtcggcggtg gggtcatagg ccttgaccgc ctcgatcaac tcgaactggc
     2101 gctggatcgg cacgcgtttc accaccggct gagaatcagc cggtggtgaa actggatcat
     2161 tattattggc gacgttttgc agcggcgggg gcttgccggc ccgcgccggt aggatcgtga
     2221 taccgttggc gggttccgcc gtcagtaacg ttcctcctgc ccgccgtccc ggtcgctctg
     2281 cagggcgcgg atcagctcct gctcggccat gttcatgtgc tgcggctcag ccagcagccc
     2341 gacgatttca gcttcgtcct cggcttccgt acgctcatcc acgcgttgca gcgccacgat
     2401 gacggtttcc agcagttcag ccggcatgac cgtttcgtcg gcgatttcac gcagggcgac
     2461 gacggggttc ttgtcgttgt cgcgatccag agtaagggcg gcgccgttgg cgatcgcgcg
     2521 agcgcggttg cccgcaagca gaaccagacc gaaacggttc ggcacctttt ggatgcagtc
     2581 ttcgacagtg acgcgagcca taggaatcct tcgaggtcgt cgggagaacc gcgcccaata
     2641 caggtaaggc ctgatttgtg caacgccaga ggggcgattg tgaggcgacc tttcaggagg
     2701 cgatgcgcgc atccgtcccc acggttgccg tcaacgccag cgccgacgcg aagccgccgc
     2761 ccggatggcg ccagtccggc gcgatctccg ccaccggccc catgacgaac agcctgtcgg
     2821 cggcgcgcgg gtgcggcagg atcaagccgt cgcaatcgcc acgttcgcga ccataggcga
     2881 tcaggtcgag atccagagtt cggggtgcgt tctgggtcga gcgccggcgt ccgaacatct
     2941 cctcgatccg ccctagcgcc cccatgagag cgtgggggtc cagcgttgtg cggacgatga
     3001 ccacgccatt caggaacggc gggtcggtcg ggtcgggcca ggcttgcgac gaccaccacg
     3061 acgaccgggc cacgacatcg atcccttcgg accgaaaccg cgtcagagca gcctcaagtg
     3121 cgtcgcggca ggacgaccag acccccttgt cattgcaacc gagggcgacg atgacggctg
     3181 catccaggtc gaggtcgccg tctatattga cggtgaggcc gttgacggcc gggttcgata
     3241 ttacgccgga gccttgcatt gtacccaacc gctcgcatcg ccctgttcat cgacggggcc
     3301 aatctttact cggcggcacg ggcgctgaac gtcgatctgg atttccgaaa actggtcgag
     3361 tcgttccgcg aaaaggccgt ccttgttcgc gcctactatt acacggcggt gatcgagggc
     3421 gaagagttct ctccgatccg gccgctggtc gactggctgg gctacaacgg cttctcagtc
     3481 gtgaccaagc cggtgaagcg gttcaccgac gcccaggggc acagcaagct gaaggggaac
     3541 atggacatcg agatcgcggt ggacatgatc gagatggctc cgcacctcga cgaaatggtg
     3601 ctgttctcag gcgacggcga cttccgcagg ctggttcagg ccgtgcaggc gaagggcgtg
     3661 cgggtaaccg tcgtgtccac caccagaagc cagccgccga tgatcgctga cgaactgcgc
     3721 cgtcaggccg acgactatat cgatctggcg gaccggctgg gtgattgggg ccggccgaga
     3781 acctcgacgc cctccccttc cgtgcgcacc ttcggagacg aggtttagcg gcccgggatc
     3841 agctcgttct ggacgcgacg gcccagcggg tcgcgggagg cccagcgttg gcccacaccg
     3901 gcctcgatca ggggcgaaca gaaacgcgag atcatcatga acgacgggcg aatgccgttc
     3961 agacatattt tttgcccctc cacgcgccag ctggcggtga tccggcgacc gtccgagaac
     4021 tgggcgctat agacgccgtc acggttgaac caatgcttaa cccagccgcc gtcagggtag
     4081 tgagacacga cggtgttgcc gaacacggag tcgatatcag ccgaagccgg tgacgcaaac
     4141 atgcccgcca atagtgctgc agcgcacaaa aagcccttca tttcaaagcc ccgcaccttc
     4201 cagaggttaa ctatcgttaa tcaatcggga tttgcaacac ccgtcaaagt aggggccgga
     4261 aagccgtgaa tacttgactc tcgcgagggg gcttctatat ccggcccacg ttcggaacag
     4321 gtctggtgaa ttcgcgccgc ccgctcgccc acagaggggc ggttccgcgc accgcacacg
     4381 cactcaagat gggtgcgcca tctgttccca aggatccatg acagaattcg caaacctggg
     4441 cctctcgccc acgacactgc aagccgtttc cgacaccggc tatacgatcg ccacgcctat
     4501 tcaggctc
//
LOCUS       DASVTM010000080        12586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_486760,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000080 DASVTM010000000
VERSION     DASVTM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12586
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486760"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VF633_02155"
     CDS             complement(<1..395)
                     /locus_tag="VF633_02155"
                     /inference="COORDINATES: protein motif:HMM:NF023345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469890.1"
                     /translation="MRFAIFFGSLALAAAVLPVYAQDEASASLTALPAVNSSAIVAEV
                     RRVIGERYVLPERRPALDAVMAEGLATGRYDVTDGAVLAERLNADLEKTGQDLHLWFR
                     FDPREAGIIASGSVEQPGANPAFERLIRNA"
     gene            complement(515..973)
                     /locus_tag="VF633_02160"
     CDS             complement(515..973)
                     /locus_tag="VF633_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8469891.1"
                     /translation="MNQGLGTQLRHLLELLDGAVTEAYARESLRYRPRYTPVMRALIA
                     SEPRAISQIAEAAGISQPAATQTVSLMIRDGLVSSAPGTDARQRMIRLTPAGRDLLPK
                     LQGCWRATTAAAADLDAELCSPLSQVLADAIAALERKSFGDRLAEARPPT"
     gene            complement(1014..1442)
                     /locus_tag="VF633_02165"
     CDS             complement(1014..1442)
                     /locus_tag="VF633_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8469892.1"
                     /translation="MTDDTDLDLLSENAFANMDVVRPVLDKIADKWTILILTVLCPRP
                     SRFNAIKRRLGGITHKALADALKRLERNGLVTRTVLPTTTPIGVEYAITPLGHSLRQP
                     FEALCAWALENAECVQDASASYDRIKAETPAALSPSRLYT"
     gene            1524..2255
                     /locus_tag="VF633_02170"
     CDS             1524..2255
                     /locus_tag="VF633_02170"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8469893.1"
                     /translation="MARMKDKIALVVGGAKGIGLATAQKLAAEGALVFLTGRDRAEVD
                     AAAETIGHGARGLVADAGRAEDLIGVVEAVMEAHGRIDALVLNAGISEPGPLGDIAAD
                     HVDRLFGVNVRGPVLGLQAALPAMKDGASVVLIGSVAGVLGIPGYGTYAATKAALRSY
                     ARSWTVELAPRGIRVNVVAPGPTDTAMMAVVPDDIRASLIAPIPLGRMARPDEVAAAA
                     VFLLSDEASFIAGSELHVDGGMGQV"
     gene            complement(2713..3681)
                     /locus_tag="VF633_02175"
     CDS             complement(2713..3681)
                     /locus_tag="VF633_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HEX8469894.1"
                     /translation="MRAILIAAALMAGITPAATALAQTVAAVPAPASPFEHDRQSILA
                     QAGQYHVHFDMRENVSFNADYDPMEGKLSGGREIVRVVYDKGNHISLQHILVMEHEGQ
                     TMVIKHWRQDWVYQPETVLTYAGPNQWTLTPVSLDARQGAWSQTVWQTDDSPRYGGLG
                     RWTYANGMKVWTSEPTWRPLARRDAVLNPVYDRYLGTNRHALTPNGWVHIQDNVKMNG
                     RAGGEPVAIVQEDVANTYDRSTSYSPKPGDDYWAATKDYWAAVRDAWDAVIARDNGVH
                     VEEEAQTGVVTGPPLMDLAQKIQDGELTTEAAIGLARTTIETATRD"
     gene            complement(3725..5854)
                     /locus_tag="VF633_02180"
     CDS             complement(3725..5854)
                     /locus_tag="VF633_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HEX8469895.1"
                     /translation="MKKAVVLAPVTVVGTRTQRRVDDVPASVSIITADTIENNLVTDI
                     KDLIRFEPGISVPTSPARFSAALSGAGRDGNSGFTIRGMGGNRVLIIQDGVRVPDGFA
                     FGAQAVGRGGYNDLDLLKSVEILRGPASALYGSDGIAGAVSFTSKDPSDFLSSGESFG
                     ARGRVGYNSSDDSWTEGLAVAGRTGSVSGLLAYTRRDAHETENMAEVGGTGATRTQPN
                     PQEFASNAILAKLVWNANDNHTVRLTYDHFDQEMDGEALSSYSAIVTGLTAHDETQRD
                     RLSLDWRFDDVFGLDGSVSAYWQDARTRQFTYEDRTAAVDRTRDVTFDNTVWGMAAQS
                     SKIFRGEGVQHRITIGGDWSRTTQRGIRDGTVPPVGEPFPARPFPETKYDLAGLFIQD
                     EIELLGGRLTIIPALRYDWYELTPEADALFPAPSEGQNDSHLSPKIGAIYWIDDHFSV
                     FANYAQGFKAPSPMQVNNFFANPVFGYESIPNPDLTPETSESVEAGMRFRNLNAFGGN
                     LSLSTTAFHTNYDDFITQIVVRGTGVPGVDPLIYQYVNLTEVKIWGLEARGDLRFDNG
                     ISFIVAASFADGDQETDGAKGKLTSIDPIKVVGGLSYAAPSGVWGASATGTWSDKKDR
                     SGCGGGNCWQGEEFAILDLTGFWNVTEKATLRAGLFNVLDESYAWWSDVRGLSRGGTV
                     MAPTDPAALGAFTQPGRNVGLSLNVRY"
     gene            6282..7736
                     /locus_tag="VF633_02185"
     CDS             6282..7736
                     /locus_tag="VF633_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEX8469896.1"
                     /translation="MIQDHHFLTVRAACRLLATTASIGLMAVAGAARAQDVGAPRWLA
                     GDHHVHSRFSATYAADPAHPDEMPQPTLGGDGPHTIIQNGAMAGQFGLSWMVSTDHGG
                     PHHSELNAAQAYPELLESRARTPDVIQFYGMELDTPGSEHSSLIIPIHDGERDMLRGI
                     ESRWGSREPWPADASHDTRAAMLDLLRTVDRYTPRPVIIANHPSRSAEGFGVWGEHDA
                     ASLRDWQDAAPRVAIGMEGAPGHQATSLKPDGQRGIYRRYPTMGGFDQMTATVGGLWD
                     AMLGEGRHWWITATSDSHRHFSEGGDDFWPGEYSKTWVMAKPEPDAILEGLRSGQIFV
                     STGDLVTEMDLTVAGGGSEARMGQDLSIAKGSDVVVTIRLRDPEAPNAGGRTPDVKRI
                     DLIAGDVTAPSQDRTVDKTADTRVERRFTDADWTRDGEMLTVRYVLSAVDRPLYLRVR
                     ATGGTELEPTADPAGEDPWSDLWAYGNPVFVRLR"
     gene            complement(7733..10702)
                     /locus_tag="VF633_02190"
     CDS             complement(7733..10702)
                     /locus_tag="VF633_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8469897.1"
                     /translation="MPTTRALAVAAAAAFAVAAPAPAWAQVPTLIDIPAQDARSALLA
                     LCLRTGCAVSFVADSGAVHRTKAVRGARSWQTALAGMLEGSGLGYRILDSRSARVWVN
                     PRPVQPAPEPEPTVLRPILVIGQFAEGIDTALSEKRRADRIQDSVSAARIGELPAANL
                     AEALQRIPGVAIEREVGEGQFVSVRGLGPLFQSVTLNGAPVAFNENIRNSTQSGRQFR
                     FRALSADLLAGATLTKSATADLVDGGIGSNIDIRTVRGLEGPTFISGQAVTHHEARSG
                     DTTGEFSLSGRWRDRNRRLGFVAGASSQDRNVQFDRLQTQRYREMILEGRPVSVPNDI
                     RTTLEREDRTRVSLFAGAQWRPNDHVTIDFDTLASRFENAIREDRIGYDLGERLTNGS
                     AVPGSIRVKDGVLTGVELTNGRISNNLEVSNQIHDNVASSLSLEADVAGWLLQPRLSY
                     SRALSRLATPLQRIGAVSDEGIAYGFDFGADPLGDRRIVRLRTSLDLTDPLALTWSRY
                     GVRAIDVDDSDVTLQIAADRPLDRSIGGVRIEALRIGAQVSDRGRDYQRRDREAALRP
                     GATVDPAFFDVLLPANAFSGVIRDGQGPWIAADFAHFRDAFVVPGEYGGVVFSTADLT
                     PTGTDLQGSYGVSETLAAAYARLDLSGFIGGLPASGNIGVRGVATRTEVIGSILGVDQ
                     AGALQVRTLAHRGSGMDWLPSTNLAVELGPRTLVRLAASRTITRPSLADLGGATVPAS
                     TLVGGIYERGQAEIDHPSPSTIFTGVGGNPDLKPYSATSIDVSFERYFEEFGAFSVAV
                     FHKVVDDYVLVVNQTERLTFATRSGPPVIADVMMSRPRNVGRATVTGLETGYSRRWSS
                     GLGVWASATLTDATSREARGVATSDLQGVSRFSWSVSPFVERGPLEAHLSWTWRSPFR
                     SIADLQGGGVTAFVVDEAGVLDFAASYRLAPKVTAFAEASNMTDAVDVAYDGIRSRPL
                     QITRSGRSFGLGLKFSL"
     gene            complement(10780..11778)
                     /locus_tag="VF633_02195"
     CDS             complement(10780..11778)
                     /locus_tag="VF633_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX8469898.1"
                     /translation="MLDRLTNLFEPTPRTGEAWLVRMGRPQVSTRDLTAFEAWLDGDN
                     TRLDEYQALKATQRQARTLKADLTAELSQIPRQRRTSKSASRGLLIGGPVLAALSVAV
                     LLVAVWGDLPFLHPADPMAGAAIYSTTVGEIRQVQLADGSQVTLDTGSAIRVVLAEDA
                     RHVILDRGRAYFDVFHDAARPFDVALADRQVTVTGTRFTTALAGGAASVALLEGSVSL
                     SSKSGPVLHMRPGDEVSYHGGLMAQTVNHVDPAETAPWRNRQLVFRDEPLSEVLAELS
                     RYTPVHLKMDDPALRRLRVTAVFPLDGDSSIIERIDQLLPVSTGSSEAGVVTVRPE"
     gene            complement(11864..12343)
                     /locus_tag="VF633_02200"
     CDS             complement(11864..12343)
                     /locus_tag="VF633_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8469899.1"
                     /translation="MRQELVGFLTRRGQAIETAEDIVQETFLRFHRAGNHIADVDARP
                     LIFVIAKNLLKDHWKSSGREQGRRAPLSPDDLEMTGQAMADDGPAPDRRILGREQLDQ
                     ALEVIRNLPPRTRDAFLLHRFEDLTYRQIADRLGVSVSMIEKHLAEALKRLKSARDH"
BASE COUNT         2222 a         3890 c         4251 g         2223 t
ORIGIN      
        1 gcattgcgga tcagccgctc gaacgcgggg ttggcgccgg gctgctcgac cgacccggac
       61 gcgattatgc ctgcctctcg cggatcgaag cggaaccaaa ggtgaaggtc ctgtccggtc
      121 ttttccaggt cggcgttgag ccgttcagcc agtacagcgc cgtccgtgac gtcatagcgg
      181 cccgtggcga ggccctcggc cattaccgca tcgagcgcgg ggcggcgctc aggcagaaca
      241 tagcgttcgc cgatcacacg acgcacttcg gccacgatcg ccgaactgtt gaccgcgggc
      301 agggcagtca gcgaagcgga agcctcgtcc tgggcataga caggcaaaac ggccgcggcg
      361 agcgcgagag acccgaaaaa gatggcgaac cgcatgaata tctccctgat tgaccggatt
      421 aaattatgaa acgattgatg tttcgggcgt ccacgaggct ggccatgggc gcgggttgtt
      481 gtcttgaacc ttgacgggga acgtcgacct gacgtcaggt cggagggcga gcctcggcca
      541 ggcgatcacc aaacgatttc cgctctagcg cagcgatcgc atcggccaga acctgcgaca
      601 gagggctaca cagctctgcg tcgagatcgg ccgcggccgc agtcgtggcg cgccagcacc
      661 cctgcagttt cggcaacagg tcccgtcccg ccggcgtgag ccggatcatc cgctgacgcg
      721 cgtccgtgcc tggcgccgac gataccaggc cgtcgcggat catgaggctc acagtctgtg
      781 tggctgcagg ttgggagatg ccggcggctt cagcgatctg actgatggca cggggctcgg
      841 atgctatcag cgcgcgcatt accggggtgt agcgcgggcg atagcgtaag gactcgcgcg
      901 cataggcctc agtgaccgcc ccatcgagca gttcgagtaa atggcgaagc tgggtgccga
      961 ggccttggtt cattcagttt ccataagcac ttatggaaac tctgtcgcgc caattatgtg
     1021 taaagccgcg aagggctgag cgctgccggc gtctcggcct tgatgcggtc ataggatgcg
     1081 ctggcgtcct gaacgcactc ggcgttttcg agggcccagg cgcacaaggc ctcgaacggc
     1141 tggcgcagcg aatgaccgag gggagtgatc gcgtattcga cgccgatcgg cgtcgtcgtc
     1201 ggcaggacgg tgcgggtcac aaggccgttc cgctccagcc tcttgagcgc gtccgccagc
     1261 gccttgtgag tgatgccgcc gagccgacgc ttgatcgcgt tgaaccgcga aggccggggg
     1321 cacagaaccg tcaggatcag gatcgtccac ttgtccgcga tcttgtcgag cacgggccgc
     1381 acgacgtcca tgttggcgaa cgcgttttcg gagagcaggt cgaggtcggt atcgtcggtc
     1441 atatctaggc tgtttcaggt gcgttattga tcgcagatac acgcggtata tctgatcgcc
     1501 atcgtagaac aaaggacatc acgatggctc gaatgaaaga caagatcgct ctggtggtcg
     1561 gaggcgccaa aggcatcggc ctggcgactg cgcagaagct ggcggctgag ggcgctttgg
     1621 tcttcctcac cggccgggac cgcgccgagg tggacgctgc cgcggagacg atcggccatg
     1681 gtgcccgggg cctcgtcgcc gacgccggtc gggcggagga cctgattggc gtcgtcgagg
     1741 cggtgatgga ggcccacggt cgcatcgacg cactggtgct gaacgccggc atctccgaac
     1801 ccgggcctct gggcgatatc gccgccgacc atgtcgaccg gctgttcggc gtcaatgttc
     1861 ggggtcccgt tctgggcctg caggccgcgc ttcccgcgat gaaggacggc gcttcggtgg
     1921 tcctgatcgg atcggtcgcc ggagtgctgg ggatcccggg ctacgggacc tatgcggcga
     1981 caaaggcggc gctgcgatcc tatgcccgga gctggacggt ggaactggcc ccgcgcggaa
     2041 tccgggtcaa tgtcgtcgcg cccgggccga ccgacaccgc catgatggcg gtcgtgcccg
     2101 acgatatccg cgcctcgctg atcgcgccga tccctctggg ccggatggcg cgacccgacg
     2161 aggtcgcagc ggcggcggtc ttcctgctca gcgacgaggc cagcttcatc gccggctcag
     2221 agctacacgt cgacggcggc atggggcagg tttgaccaca ccgactacag cggattttcc
     2281 gcaagtctct gacaaggggt gtcgcggccc gtttccggga cggtcgggcg attgaccgtg
     2341 tcgcgagctc aggcaagcgg catcggtagt acggccgtca cagagacgtt ttagcgcggg
     2401 cttgctggga gctaccaagt ttcgtcctgt tgcttgagca tgaaatctaa cgatctcgaa
     2461 cgtctgttct cgggaggaac ccgactttcg ctcctggcgc ttctgagatc tttaggatca
     2521 accgcagttc gtgactccga accgcggtcc ggaaggtccg cttgtggaac cgcacctgct
     2581 cgggcgcgag gatcccgcct gactcaatag agtagtcggg gttgcctggc tgtcgatcca
     2641 tgaagaacag tcgaaacgtg gttgccaacg ctgaagggtg gatgggcttg cccatccacc
     2701 cttcagcggg tctcagtccc gggtggcggt ctcgatcgtc gtccgggcca gcccaatggc
     2761 ggcttcggtc gtgagctcgc cgtcctggat tttctgggcg aggtccatca gaggcggtcc
     2821 ggtgacgacg ccggtctgcg cctcttcctc gacatggacg ccgttgtcgc gggcgatgac
     2881 ggcgtcccag gcgtcgcgga cagcggccca gtagtccttc gtcgcagccc agtaatcgtc
     2941 gccgggcttg ggcgagtagc tggtcgagcg gtcgtaggtg ttggcgacgt cttcctggac
     3001 gatggcgacg ggctccccgc cagcccgacc gttcatcttc acattgtcct gaatatggac
     3061 ccagccgttt ggcgtcaggg cgtggcggtt cgttcccaga tagcggtcat agaccgggtt
     3121 cagcaccgcg tcgcgacgcg ccagcggtcg ccaggtcggc tcggaggtcc agaccttcat
     3181 gccattggcg taggtccagc gtcccaatcc accgtatcgg ggcgaatcgt cagtttgcca
     3241 cacggtctga gaccaggcgc cctggcgcgc gtccaaggaa accggcgtca gcgtccactg
     3301 gttcggaccg gcgtaagtca gaaccgtctc gggctgatag acccagtcct gccgccagtg
     3361 cttgatgacc atggtctggc cctcatgctc catgaccagg atatgctgca ggctgatgtg
     3421 gttgcccttg tcatagacga cgcggacgat ctctctgccc ccggacagct tgccctccat
     3481 cgggtcgtag tcggcattga agctgacgtt ctcgcgcatg tcgaagtgga cgtggtattg
     3541 gccggcctgc gccaggatcg actggcgatc gtgctcgaac gggctggcgg gggccggaac
     3601 ggccgcgacg gtttgagcca gagccgtcgc ggcgggcgtg atgccagcca tcagggccgc
     3661 ggcgatgagg attgcgcgca tggggcttgc cttctttggt gaaattgtca tcgggttggc
     3721 ggggtcaata tcggacgttt agcgagaggc cgacgttgcg accgggctgg gtaaaggcgc
     3781 ccagcgctgc gggatcggtc ggagccatga ccgtgccgcc gcgcgacagg ccgcggacgt
     3841 cgctccacca ggcgtagctt tcgtccagga cattgaagag tccggcacgc agcgtggcct
     3901 tctcggtcac attccagaaa ccggtgaggt ccaggatggc gaactcttcg ccctgccagc
     3961 aattgccgcc gccgcaaccc gagcggtcct tcttgtccga ccaggtgccg gtcgccgagg
     4021 cgccccagac gcctgacggt gcggcgtagc tcaggccgcc gacgaccttt atcgggtcga
     4081 tactggtcag cttgcccttg gcgccgtctg tctcctgatc gccgtcggcg aacgaggccg
     4141 cgacgatgaa cgagatgcca ttgtcgaaac ggaggtcgcc ccgcgcttcg agtccccaga
     4201 tcttcacctc agtcaggttg acgtattgat agatcagcgg atcgacgccg gggacacccg
     4261 tgccgcgcac cacgatctgg gtgataaagt cgtcgtagtt ggtgtggaag gccgtcgtgg
     4321 agagcgacag gttgccgccg aacgcgttca gattgcggaa gcgcatgccc gcttcgacac
     4381 tctcgctggt ttcgggcgtc aggtccgggt tggggatcga ctcatagccg aatacggggt
     4441 tggcgaagaa gttgttcacc tgcatgggtg aaggggcctt gaagccttgg gcgtagttgg
     4501 cgaagacgct gaagtggtca tcgatccagt agatggcgcc gatcttgggc gacaggtgac
     4561 tgtcgttttg gccctctgaa ggggctggga acagggcgtc ggcctcgggt gtcaactcgt
     4621 accagtcgta gcgcagggcc gggatgatag tgagacggcc gcccaacagc tcgatctcgt
     4681 cctgaataaa caggccggcc agatcgtatt tggtctcggg gaatggtcgg gcggggaacg
     4741 gttcgccgac cggtggaacg gtgccgtcgc ggatgcctct ctgggtcgtg cgcgaccagt
     4801 cgccgccgat ggtgatgcga tgctgcacgc cttctccgcg gaagattttt gagctttgcg
     4861 ccgccatgcc ccagaccgtg ttgtcgaacg tgacgtcgcg ggtgcgatca accgccgccg
     4921 tgcggtcctc ataggtgaac tgacgcgtcc gggcgtcctg ccagtaggcg gagaccgaac
     4981 cgtccaggcc gaagacgtca tcgaagcgcc agtccaggct aagccgatcg cgttgggttt
     5041 catcgtgcgc cgtgaggccg gtgacgatgg cggagtagct gctgagcgct tcgccatcca
     5101 tttcctgatc gaagtgatcg taggtcaggc ggacggtgtg attgtcgttg gcgttccaga
     5161 ccaacttggc cagaatggcg ttggaggcga actcctgtgg gttcggctgg gtgcgggtcg
     5221 cgcccgtacc gccgacctct gccatgttct cggtctcatg ggcgtcgcgg cgggtgtatg
     5281 ccagaagccc agacacggag ccggtgcggc ccgccacagc caggccctcg gtccagctgt
     5341 cgtccgacga gttgtaaccg acgcgacccc gggcgccgaa gctttcgccg ctggagagga
     5401 agtcggaagg gtccttggag gtgaagctga ccgcgccggc gatgccgtcc gagccgtaga
     5461 gggcggaggc cggaccgcgc aggatctcga cagacttgag gaggtccagg tcgttatagc
     5521 cgccacgccc aacagcctgg gcgccgaaag cgaagccatc gggcacgcgc acgccgtcct
     5581 gaatgatcag cacccggttg ccgcccatcc cgcggatggt gaagcccgag ttgccatcgc
     5641 gcccggcacc ggaaagggca gccgagaaac gggcagggct ggtcggaacg ctgatgcccg
     5701 gctcgaagcg gatcaggtcc ttgatgtcgg tgacgaggtt gttctctatg gtgtcggcgg
     5761 tgatgatgct gacgctggcc ggaacatcat cgacgcgtcg ctgcgtgcgt gtgccaacca
     5821 cagtgacagg cgcgaggacc accgcctttt tcaagtcgct gggctcctca gctcctgccg
     5881 ggaaagcagc acaggacagg gcagcaagcc caacggaaaa catcagaatc gaacgcatgg
     5941 ttgcagcccc ggttgaagag ggttgcgcaa ttactgcgaa cgattatcag aatcaaccgc
     6001 aatagcggat aatctgtgcg tgaacgccgg atcgcaggat tctgctccgg cgggtgggcg
     6061 actgaccagt cgggagtgcc tggctgaccg ccgtccagcg accacccgag cagcccgcat
     6121 cagaagcatc ggccggaaaa gtcgctatcg tgccgggccg gtaccgacgg agtcccctcg
     6181 aacaatttcg aacgtcggcg ttgaggcgtt cgggatgtga gtcgttgtgt accttaaccg
     6241 gtccgagcag gaacccggcc ctgagccgcg gggattccag catgatccaa gaccatcatt
     6301 tcctgacagt tcgtgcagcc tgtcggctcc tggcaaccac agcatccatc ggtctcatgg
     6361 cggtcgcggg cgccgcccgc gcccaggatg tcggtgcacc gcgttggctg gcgggcgacc
     6421 accacgtcca tagccggttc agcgcgacct atgccgccga cccggctcat ccggacgaga
     6481 tgccccaacc caccctcggt ggcgacggtc cccatacgat catccagaac ggcgcaatgg
     6541 cgggacagtt cgggttgagc tggatggttt cgaccgacca cggcgggcct catcactccg
     6601 agctcaacgc cgcgcaggcc tatccggagt tgctggagtc gcgcgcccga acgccggacg
     6661 tcatccagtt ttacggcatg gaactggaca cgccggggag cgagcattcg agcctgatca
     6721 ttccgatcca cgacggcgaa cgcgacatgc ttcgggggat cgagtcccgc tggggctcgc
     6781 gcgagccatg gcccgccgac gcgagccacg atacacgcgc ggcgatgctg gacctgctgc
     6841 ggactgtcga ccggtacacg cccaggcctg tcatcatcgc caaccaccca tcacgctcgg
     6901 ccgaaggttt cggggtctgg ggcgaacacg acgccgcctc actcagggac tggcaggatg
     6961 cggcgccgcg cgtggccatt gggatggagg gggcgccggg ccatcaggcg accagtctga
     7021 agccggacgg ccagcgcggg atttatcggc gctatccgac gatgggcggc ttcgaccaga
     7081 tgacggcgac ggtcggcggg ctgtgggacg cgatgctggg tgagggccgt cactggtgga
     7141 tcaccgcgac gtcggactcg catcgtcact tcagcgaggg gggtgacgac ttctggcccg
     7201 gggaatattc caagacctgg gtcatggcca agccagaacc cgacgccatt ctggaaggcc
     7261 tgcgatcagg ccagattttc gtctctacag gcgatctggt cactgaaatg gacctgaccg
     7321 ttgccggcgg cggtagcgag gcccggatgg ggcaggacct ttccatcgcg aagggctccg
     7381 acgtcgtcgt gaccatccgc ctgcgggatc cggaggcgcc gaatgccggg ggtcgcaccc
     7441 ctgacgtcaa gcgtatcgat ctcatcgccg gcgacgtcac ggcgcccagc caggatcgta
     7501 ccgtagacaa gaccgcggac acacgcgtgg agcggcgctt cactgacgct gactggactc
     7561 gcgacggcga gatgctgacg gtgcgctatg tcctctctgc ggttgatcgc ccgctttatc
     7621 taagggtccg cgcgacaggc gggaccgagc tggaacccac cgccgatccg gccggtgaag
     7681 atccgtggtc cgacctgtgg gcctatggca atccggtctt cgtcagactg cgctagaggc
     7741 tgaacttcag cccgaggcca aaggatcgtc ccgagcgggt gatctgcagc ggacggctgc
     7801 ggatgccgtc gtacgcgacg tcgacggcgt cggtcatgtt gcttgcctcg gcgaaggccg
     7861 tcaccttggg agccagccgg tatgaggcgg cgaaatccag aacgcccgct tcgtcgacga
     7921 cgaaggccgt gaccccgccg ccttgcaagt ccgcgatgga ccgaaatggg gaacgccagg
     7981 tccaactcag gtgggcctcg agcgggccgc gttcgacgaa ggggctgacg gaccaggaaa
     8041 agcgggagac cccctgaaga tcagacgtag ccacgccgcg agcctcgcgg ctggtggcgt
     8101 cggtcagggt tgcgctggcc cagacgccca gcccggaaga ccaacgtcgg ctgtatcccg
     8161 tctcgagacc cgtgaccgtg gcgcgaccga cgttgcgggg tcttgacatc atcacgtctg
     8221 cgatcacggg cggaccagat cgcgtggcga aggtcagccg ctcggtctgg ttcaccacca
     8281 gcacataatc gtccacgacc ttgtggaaga cggccacgct gaacgctcca aactcttcga
     8341 aatagcgttc gaacgagaca tcgatgctgg tggcggaata gggcttgagg tcgggattgc
     8401 cgcccacacc ggtaaagatc gtggagggcg agggatggtc gatttcggcc tggccccgct
     8461 catatatgcc gccgacgagg gtgctggcgg gcacggtagc cccgcccaga tcggcgagtg
     8521 aggggcgggt gatcgtgcgc gatgcggcga gtcgaacaag cgtacgcggt cccagctcga
     8581 cggccagatt ggtgctcggg agccagtcca ttccggaccc gcgatgagcg agcgtgcgaa
     8641 cctgcagggc gccggcctga tcaacaccaa gaatggagcc aatcacctcg gtgcgggtcg
     8701 ccaccccccg tacgccgatg ttgccagatg ccggcaggcc gccgatgaag ccggaaaggt
     8761 cgagccgggc gtacgctgca gccaaggttt ccgaaacgcc gtaggagccc tgaaggtcag
     8821 ttcccgtcgg tgtcagatca gcggtgctga agacgacccc gccgtattct ccaggcacga
     8881 cgaaagcgtc gcggaagtgt gcaaaatcgg cggcgatcca aggaccttgc ccgtcgcgga
     8941 tgacgcccga gaaagcattg gcgggaagca aaacgtcgaa gaaggcggga tcgacggtag
     9001 cccccggtcg cagggccgcc tcgcggtcgc gtctctgata gtcgcgtccc ctgtcgctga
     9061 cctgggcgcc gatccgcagt gcctcgatcc ggactccgcc gatagatcgg tccagcgggc
     9121 gatcagcagc gatctgcagc gtcacgtcgc tatcgtcgac gtcgatcgcc cgcacaccat
     9181 agcgggacca ggtcagggca agcgggtcgg taagatcgag gcttgtgcgc agccgtacga
     9241 tccgacggtc gcctagcggg tccgcgccga agtcgaaccc ataggcgata ccttcgtcag
     9301 acaccgctcc aatgcgctgc agcggtgtcg ccagtcggga gagggcgcgt gaatagctta
     9361 gtctgggttg caggagccaa ccggcgacgt ctgcctcaag cgacagactg ctggcgacat
     9421 tgtcgtgaat ctggttcgag acctcgaggt tgttgctgat gcggccgttg gtcagttcaa
     9481 cgccggtcag gacgccgtcc ttgacgcgga tgctgcccgg aacggccgaa ccattggtca
     9541 accgctcgcc caggtcatag ccgatccggt cttcacggat ggcgttctcg aaccgcgagg
     9601 cgagggtgtc gaaatcgatg gtgacatggt cgttggggcg ccactgggct ccggcgaaca
     9661 gactgacgcg ggtgcggtcc tcccgctcca gagtggtccg tatatcgttc ggtacagaga
     9721 ccgggcggcc ctcgaggatc atctcccgat agcgctgtgt ttgtagccgg tcgaactgga
     9781 cgtttcggtc ttgcgaggag gctcccgcca cgaagccgag ccgtcgattg cgatcacgcc
     9841 aacgtcccga aaggctgaac tctccggtcg tatcaccgga gcgggcttcg tgatgggtca
     9901 cggcctgacc gctgatgaac gtggggccct ccagaccccg gaccgtacgg atgtcgatgt
     9961 tggagccgat gcccccgtcg acgaggtccg ccgtcgcgga cttcgtcagg gttgcgcccg
    10021 ccagcaggtc ggcggacagc gcgcggaaac gaaactgccg tccgctctgg gtcgagttgc
    10081 ggatgttctc gttgaacgcc accggcgcgc cgttgagcgt caccgactgg aacagggggc
    10141 ctagacctcg cacgctgacg aattggcctt cgccgacctc gcgttcgatc gccacgccgg
    10201 gaatgcgctg aagcgcctcg gccagattgg cggcagggag ctcaccgatt cgggcggcag
    10261 agaccgagtc ctggatcctg tcggcgcgac gcttttcgct gagggctgta tcgatgcctt
    10321 cagcgaactg gccgatcacc aaaatgggcc gcagcacggt gggttcaggt tcaggggcgg
    10381 gctggacggg ccgggggttc acccatactc gcgcggaccg gctgtcgaga atccggtagc
    10441 cgagcccgga accctccagc atccccgcga gcgccgtttg ccaactgcgc gcgccccgca
    10501 ccgctttagt gcgatggacg gcgcctgaat ccgcgacgaa actcacggcg cagccggtcc
    10561 gcagacacag ggccaataac gccgaacggg cgtcttgagc ggggatgtcg atcaacgtcg
    10621 gtacctgcgc ccaggccggc gcgggagccg cgacagcaaa cgcggcggct gcggcgacgg
    10681 cgagcgctct ggtagtcggc atgcgacggc tgacgggcgg cgcgacgtga tctgtcgctc
    10741 acaccggtct agacggtcca gatgacaggt ctgcgatagt tattccgggc gaactgtgac
    10801 gacgcctgcc tctgaggacc ctgtagagac cggcagaagc tggtcgatcc gctcgatgat
    10861 agagctgtca ccgtccagcg gaaagaccgc tgtgacgcgc aacctgcgca gggccggatc
    10921 atccattttc aggtggacag gtgtgtagcg ggaaagttcg gccagcacct cggacaaggg
    10981 ttcatcgcgg aagaccagct gtcgattgcg ccacggcgcc gtctcggccg gatcgacatg
    11041 attgaccgtc tgagccatca gtcctccgtg atatgacacc tcgtctcccg gccgcatgtg
    11101 caagaccggt ccagatttcg acgacagcga cacggaccct tccagcaggg cgactgacgc
    11161 ggctccgcct gccaaagccg tagtgaaacg cgtacccgtt acggtcacct ggcgatcagc
    11221 caacgcgacg tcgaacgggc gcgcggcgtc gtggaagacg tcgaaatagg cccggccccg
    11281 gtcaaggatg acatggcgag cgtcttcggc cagaaccacc cggatcgccg aaccggtgtc
    11341 gagggtcacc tgcgagccgt ctgccagctg tacttggcgg atctcgccga ccgtggtcga
    11401 gtagatcgcg gctccggcca tgggatcggc agggtgcaaa aacggtaggt cgccccacac
    11461 tgcaaccagc agaaccgcca cgctcaacgc cgcgaggacc gggccgccaa tcagcagtcc
    11521 gcgtgatgcc gacttcgacg tccgacgttg gcgcggtatc tgcgacagct ccgccgtgag
    11581 atcggccttg agcgtccggg cctgacgctg ggtcgccttg agcgcctgat actcgtccag
    11641 acgagtgtta tctccgtcga gccacgcttc aaacgcggtc aggtcgcgcg tgctcacctg
    11701 cggccggccc atacggacca gccacgcctc acccgtgcgg ggtgtcggtt cgaacaggtt
    11761 ggtcaggcgg tcaagcatca gggcaaatgg cccctttcag agcgggtgaa gtccggggcg
    11821 atacgcccgg actgtcttct caacgacaca aatcacgacc acctcagtgg tctcgcgccg
    11881 atttcaggcg cttcagcgct tcggcgagat gcttttcgat catgctgacc gatacgccaa
    11941 gacgatccgc gatctgacga tatgtcaggt cctcgaaacg atgcagcagg aaagcgtcac
    12001 gggttcgggg cggaaggttg cgaatgactt ccagcgcctg gtccagctgc tcgcgtccaa
    12061 ggatgcgcct gtcgggcgcg gggccgtcgt cggccatggc ttggccggtc atctccagat
    12121 cgtcgggtga cagaggcgcg cgtcgaccct gctcgcgtcc tgaactcttc cagtgatcct
    12181 tcagaaggtt cttcgcgatg acgaagatca gcggacgggc gtcgacgtcc gcgatgtgat
    12241 tccctgcgcg atgaaaccgc aggaaagtct cttgcacgat gtcctcggcg gtctcgatcg
    12301 cctgtccacg tctcgttaga aacccgacca gttcctgacg catgcgctcc gtcgcggcct
    12361 gatcgcccgg acgtgcgaaa acgccccctc gcgaggagga ggcgttttct gtcgtcaggc
    12421 gtgtggtgct cagaacgtcg cccgcaggcc gaggttgaag cttcggccgt actcgtgcgt
    12481 ctggaagggg cgatcctcgt tgccctgata gaccgcctgg cgttcgtcgc tgatgttggt
    12541 gccttccgcg aagatctgga agttttcgct cacctgccaa gaggcg
//
LOCUS       DASVTM010000081         8426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_379565,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000081 DASVTM010000000
VERSION     DASVTM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379565"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="aroC"
                     /locus_tag="VF633_02205"
     CDS             complement(<1..696)
                     /gene="aroC"
                     /locus_tag="VF633_02205"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX8469900.1"
                     /translation="MSHNTFGHLFRVTTWGESHGPAIGCVVDGCPPMIPLTEADLQPW
                     LDLRKPGGSRFVTQRNESDTARILSGVFDDGNGPVTTGTPIAIEIQNEDQRSKDYGEI
                     ARAYRPGHADVTYQAKYGVRDHRGGGRSSARETASRVAAGAVARKVLGDGVVIRAGVV
                     QLGTHRIAEEAIDFGAVYQNPLFAASSEVAAEWEGYLDGVRKAGSSIGAVVALEVTGV
                     PAGWGAPLYAKLDA"
     gene            complement(707..1423)
                     /locus_tag="VF633_02210"
     CDS             complement(707..1423)
                     /locus_tag="VF633_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX8469901.1"
                     /translation="MRLTSQNLAQSFATLGLHGPTGGDALTRAFREAVKAARPDMPGG
                     DDDRFRRVIAAYRLIQEQGGALTSLAAPITTPEAPPVVVVTPLQALQGGATRIRLNGR
                     AVKVTVTAGLRTGDHVRLRGAADGADLYLPVLIRSADGLTVLGDDVHMNWPVAPRLSE
                     DGGRIEIDTHAGLRGAWITPGMMGPVKIRLRDMGLPARGGRPAGHLFVSLQPSEDAPS
                     AAEHLLARFTRVWTPDRLAA"
     gene            1549..2223
                     /gene="pdxH"
                     /locus_tag="VF633_02215"
     CDS             1549..2223
                     /gene="pdxH"
                     /locus_tag="VF633_02215"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX8469902.1"
                     /translation="MTNAAIPASPDRETYARDYAAALAANGDETIFDRAEPIGLFLEW
                     LADAKETEPNDANAMGLSTVDASGAPDSRMVLLKDIDGRGFTFFSNVESDKGLELAAH
                     PVAALLFHWKSLRRQVRVRGRVEPVTPDEADVYFATRARESRIGAWASDQSRPLPDRA
                     TLEAAIARASERFEGQDVPRPERWTGWRVVPTAVEFWRDRPFRLHDRLRFDMVDGQWR
                     RERLYP"
     gene            complement(2227..2859)
                     /locus_tag="VF633_02220"
     CDS             complement(2227..2859)
                     /locus_tag="VF633_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8469903.1"
                     /translation="MNSEIRASLTTPVQDPRRAAILCSARRPFMSSGYAATRMEPIAR
                     EAAVSTATLYAYFPSKEDLFAAVIIDAADEFERQMHNVGAGAGPARVRLTEFMMAYAG
                     FMGDPFVRSVFRLVMAERPRFRDVAMRFFDRGRSDFGRPLMTALTELQATGELKIDKP
                     SWAAGQLMGMIEHPVFFMPLVTGDEVQATRSVEQIVNDAIETFMARYGAK"
     gene            complement(2917..3579)
                     /gene="udk"
                     /locus_tag="VF633_02225"
     CDS             complement(2917..3579)
                     /gene="udk"
                     /locus_tag="VF633_02225"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX8469904.1"
                     /translation="MTILIAITGGSGSGKSTLAEAIVSALRPGAAALVREDSYYRDAA
                     SLPDFDADTFDFDDVAARDHELMAADLRRLKAGKPVTAPVYSFLHHGREPDGEPIPAA
                     EVIVVEGTHVLCTPEMTALFDIRVFVDTPADIRFIRRLLRDQAERGRTAESVIHQYLL
                     TVRPGHERLTEPSKVHADFVVADATAAVRLDDPQAVVRLVAPVLAHPLLAGMIERALR
                     PS"
     gene            3800..5434
                     /locus_tag="VF633_02230"
     CDS             3800..5434
                     /locus_tag="VF633_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX8469905.1"
                     /translation="MLGLMQDWPLTVDRILDHAKNWHGHREVVSRSVEGPIVRTTYAD
                     IHARAKRVSNALLGWGVKPGDRIATLAWNTGKHIEAWYGIMGIGAVCHTLNPRLFPEQ
                     LIYIINHAEDRMIFVDLTFVPLLEAILPHCPKVERVIVMTDEATMPATRLPLAECYEA
                     VVAGASADVIWGDFPETTACGLCYTSGTTGNPKGVLYSHRSNFIHTLLGMQSTVLGAT
                     PSEVILPVVPMFHANAWGIAFAGPAAGAKLVMPGAKMDGASIYELIVSEGVTFSAAVP
                     TVWQGLLAHLREHNLQIPTVKRVLIGGSAVPESLIRAFHDEFGIEVLQGWGMTETSPI
                     GTLSNMTPETLALPYDEQMKYRVKQGTPPLGVELKLKNYAGQEMPQDGHTYGRLMIKG
                     PTIAGAYFRSDESILDAEGFFDTGDVSTIDEMGFMQITDRAKDVIKSGGEWISSIEIE
                     NIAMGHPRVELAAVIGAAHPKWDERPVLIVKLKPGEPQDKQEHLDFLVGKIAKWWMPD
                     DVVFVDDIPLGATGKVDKKLIRERLKDYRLPTATAA"
     gene            5525..5809
                     /locus_tag="VF633_02235"
     CDS             5525..5809
                     /locus_tag="VF633_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469906.1"
                     /translation="MTNALQRTSKLACAIAVILGGSAMLQGCLVGAVAGAGVAVVGGT
                     AKVVGGVAGAGIDAVTTSDQETRQRREREQRDYEREQRRCEQRQRQGKRC"
     gene            6407..7783
                     /locus_tag="VF633_02240"
     CDS             6407..7783
                     /locus_tag="VF633_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX8469907.1"
                     /translation="MSAPRIAVIGAGFSGLLTAVHLSRRLPDAELWLIEKGSRFAEGA
                     AYSTREADHVLNVRLGNMSAFPDEPEHLSRWLATQPHWSSKGAFITRGDYGRYLRSIL
                     SDALSGARSESDSEAGSQTRSAAGSRLKLVHDEVLDLTRAGGGWQVLLGSGETLAVDV
                     AVLALGNLEPARPSGLDEAVVAAGRYIADPWRGAANLPDDARRILLIGSSLTMVDVAL
                     SHARPGRRFFALSRRGLAPRAHKAADLALTSRPYSGSPLTVLRQARRAALTSDWRAVI
                     DDLRRSARTLWRSWSRAQRGRFLRHLRPIWDVHRHRLAPAVASRIQSMIASGELSIEA
                     GRIERIEPAGTKVLVTWRRRGRGRTTTRKVDVVINCTGPLADVVASQIPLVKGLVASG
                     YGRADPDGLGLDVDEQCRVRDIDGAPQDELYAVGPMTRGAFWEVTSVPDIRIQAAEVA
                     EVIAYSAG"
     gene            complement(7831..8196)
                     /locus_tag="VF633_02245"
     CDS             complement(7831..8196)
                     /locus_tag="VF633_02245"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX8469908.1"
                     /translation="MTEFEITKAATFDAAHHFPNEPEGSIYRRMHGHSFQVEATVKGT
                     TLDGHGWVADLGALDRALKAAAEQLDHGLLNEKPGLELPTLEHLCLWFVAQLKPDWPG
                     LSRITVARPTIHEKCTLTL"
     gene            complement(8193..>8426)
                     /locus_tag="VF633_02250"
     CDS             complement(8193..>8426)
                     /locus_tag="VF633_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX8469909.1"
                     /translation="PKADAPVLQTRGAELKLVFPQGSAMPDRFEGLDFERFWLQPMDG
                     PDQVANTEAAIEYCLTHPMWRLSVQTHKYVGVR"
BASE COUNT         1462 a         2961 c         2582 g         1421 t
ORIGIN      
        1 ggcatccagc ttggcgtaga gcggcgcgcc ccagcccgcc ggaacgccgg tgacctcgag
       61 cgcgaccacg gccccgatcg acgacccggc cttgcgcacg ccatccagat acccttccca
      121 ctccgctgca acctctgacg acgcggcaaa cagcgggttc tgatagacgg cgccgaaatc
      181 gatggcctct tcggcgatcc gatgcgtccc aagctggacc acgccggctc ggattactac
      241 cccatcgccc agcaccttcc tggcgaccgc tccggcggcg acccggctgg cggtctccct
      301 agccgacgaa cgcccgccgc cgcggtggtc gcgcactccg tacttggcct gataggtcac
      361 gtcggcgtgg ccggggcgat aggcccgcgc gatctcgcca tagtctttcg accgctggtc
      421 ctcgttctgg atctcgatag cgatcggcgt gcccgtcgtg acaggtccgt ttccgtcatc
      481 gaatacgccc gacaggatac gcgcagtgtc gctctcgttc cgctgggtca cgaagcggct
      541 gccgcccggc tttctcaggt ccagccatgg ctgcaggtcg gcttcggtca acgggatcat
      601 cggcggacag ccgtcgacga cacagccgat cgccggccca tggctctcgc cccaggtcgt
      661 cacgcgaaac agatggccga aggtgttgtg ggacatgcgg cctcgcttaa gccgccagac
      721 gatccggcgt ccacactctc gtgaaccgcg ccagcaggtg ttccgccgca gacggggcgt
      781 cctcggacgg ttgaaggctc acgaacagat gccccgcggg tcggccaccc cgcgccggca
      841 gccccatatc ccgcagtcgg atctttactg gccccatcat gcccggtgtg atccaggcgc
      901 cgcgcagccc ggcgtgagtg tcgatctcga ttcgaccgcc atcctcgctc aggcgcggcg
      961 cgacgggcca gttcatgtgc acgtcgtcgc ccagaaccgt gaggccgtcg gccgaacgga
     1021 tcagcaccgg taaatagaga tcggcgccgt cggcggctcc acgcagtcgc acatgatcgc
     1081 ccgtccgcag ccctgccgta acggtgacct tgaccgcacg accgttcaac cggatccggg
     1141 tcgccccacc ttgcagggcc tgcagcggcg ttacgactac gaccggcggg gcctcaggcg
     1201 tagtgatcgg ggccgcgagg gatgtcaggg cgccgccttg ctcctggatc aggcgatagg
     1261 ctgcgatcac ccgccggaac cggtcgtcat caccgccagg catgtccggg cgcgccgctt
     1321 tgacagcctc ccggaatgcc cgtgtcaggg cgtcgccgcc cgtcggtccg tgcaaaccga
     1381 gagtggcgaa agactgggcg aggttctggc tggtcaagcg catcgcacag ccttgtcgcg
     1441 gacatggtca agaccgggtt aaccgcgttc cacgcttcac cggtccagca tcgcctttct
     1501 tgatccagaa caatgccaat cgctgctcca gtggttatat tgccggccat gaccaacgcc
     1561 gccattcccg ccagccctga ccgtgagacc tacgcgcggg actatgccgc agccctcgcc
     1621 gctaacggcg acgaaacgat tttcgaccgg gccgagccga tcggcctatt cctcgagtgg
     1681 ttggccgacg cgaaggaaac cgagcccaac gacgccaatg cgatgggcct gtcgacggtc
     1741 gacgcctccg gcgcgccaga cagccggatg gtgctactga aggatatcga tgggcgcggc
     1801 ttcaccttct tctccaacgt cgagagcgat aaggggctgg agctcgccgc ccatcccgtc
     1861 gccgcgctcc tgttccactg gaagtcactg cgccgtcagg tgcgggttcg cggccgggta
     1921 gagccggtca cgcctgacga ggccgacgtc tatttcgcca cccgcgcgcg cgaaagccgc
     1981 atcggcgcct gggcttctga ccagtcccgc cccctgcccg atcgggccac gcttgaggcc
     2041 gccatcgctc gcgccagtga acggttcgag ggccaggacg tcccccgccc ggaacgctgg
     2101 accggctggc gagtcgttcc aacagccgtc gagttctggc gcgatcgccc cttccgcctg
     2161 cacgaccggc tccggttcga tatggtggac gggcagtggc gtcgcgagcg cctctacccc
     2221 tgacgactat ttcgcaccgt aacgcgccat aaaggtctcg atagcatcgt tgacgatctg
     2281 ttcgaccgac cgcgtcgcct ggacctcgtc cccggtgacc agcggcatga agaagacggg
     2341 gtgttcgatc atccccatca actggcctgc cgcccatgag ggcttgtcga tcttcagttc
     2401 gccagtagcc tgaagttccg tcaaggcggt catcaggggg cgaccgaagt cgctgcggcc
     2461 gcggtcgaaa aagcgcatcg ccacgtcacg aaaacggggt cgttctgcca tgacgagcct
     2521 gaacaccgat cggacgaatg gatcacccat gaagccggcg taggccatca tgaactcggt
     2581 cagtcgcaca cgggccggtc ccgcgcccgc cccgacgtta tgcatttgcc gctcgaactc
     2641 gtcggcggcg tcgatgatga cagccgcgaa caggtcctcc ttggagggaa agtaggcgta
     2701 caacgtcgcg gtggagaccg cggcctctcg cgcgatcggt tccatccggg tcgctgcgta
     2761 gccagagctc atgaacggtc ggcgcgcgct gcacagaatg gccgcccggc gagggtcttg
     2821 tacaggtgtg gttagggaag ccctgatttc gctgttcatt ttgaccgact gccgttctgc
     2881 gcaacctgtc tcgaaagctc tgcccccgca acataattag cttggccgta gagcccgctc
     2941 gatcattccg gccagcaacg gatgcgccag caccggcgcc accaggcgca ctaccgcctg
     3001 cggatcatca agccgcaccg cagccgtagc atccgcgacc acgaaatccg catgcacctt
     3061 ggacggctcg gtcaggcgct catgtccggg ccggacggtt agcagatact ggtggatcac
     3121 ggactccgcc gtccgcccac gctcggcctg atctcgaagc agacgccgga tgaatcggat
     3181 gtcggcaggc gtatcgacga acacccggat gtcgaacagc gcggtcatct cgggcgtaca
     3241 caggacgtgc gtgccctcga cgacaatcac ctcggccgcc gggataggct cgccatcagg
     3301 ctcgcgcccg tggtggagga aggaatacac cggcgcggtc accggcttgc ccgccttcag
     3361 ccgcctcaag tcagccgcca tcagctcgtg atcccgcgcc gccacgtcgt cgaaatcgaa
     3421 cgtatccgcg tcgaagtccg gcaagctcgc cgcatcgcga tagtaggaat cttcgcgcac
     3481 cagcgcggct gcgccagggc gcagtgccga aacgatcgct tccgccagcg tgctcttgcc
     3541 ggagccggag ccccccgtaa tcgcgatcag aatagtcatt gggagggctt cgccggggtt
     3601 agggggtagg gagtagggct tagggaagac cgttcgtttc gtccatgccg atgcccccct
     3661 accctcccta ctccctaagc cgtaacccct actccagcag cggcaagctg ccccaaggtc
     3721 acgtttgctt atcagcaatc acctgactag cgtgtctcca agcgccgcga tcagccggtg
     3781 caaactccga ggaagtgcaa tgctgggttt gatgcaggac tggccgctga cggtcgacag
     3841 gattctcgat cacgcgaaga actggcatgg ccaccgcgag gtcgtgagcc gctcggttga
     3901 ggggccaatc gttcgcacga cctacgccga cattcatgcc cgcgcgaaac gcgtgtcgaa
     3961 cgccctcctc ggctggggtg taaagcccgg cgatcgcatc gccaccctgg cctggaacac
     4021 cggcaagcac atcgaggctt ggtacggcat catgggcatc ggcgcagtct gccacacgct
     4081 gaacccccgc ctgttcccgg aacagctgat ctacatcatc aatcacgccg aagaccggat
     4141 gatcttcgtc gacctcacct tcgtgccgct gctcgaagcc atcctgccgc actgccccaa
     4201 ggtcgagcgg gtcatcgtca tgaccgacga ggcgaccatg ccggcgacca ggctcccgct
     4261 cgctgaatgc tatgaagccg tcgtcgccgg agcctccgcg gacgtgatct ggggtgactt
     4321 cccggaaacc accgcctgcg gcctctgcta cacctcaggc acgaccggga accccaaggg
     4381 cgtcctgtat tcgcaccgct cgaacttcat ccacaccctg ctgggcatgc agtcgacggt
     4441 cctcggcgcg acgccgtccg aggtcatcct gccggtcgtg ccgatgttcc acgccaatgc
     4501 ctggggcatc gccttcgccg gtccggcggc aggcgccaag cttgtcatgc cgggcgccaa
     4561 gatggacggc gcctcgatct atgagctgat cgtctccgaa ggcgtgacct tctcggccgc
     4621 cgtgccgacc gtctggcaag gcttgctggc ccatctgcgt gagcacaatc tgcagatccc
     4681 gacggtcaag cgcgtgctga tcggcggctc ggccgtgccc gaaagcctga tccgcgcttt
     4741 ccacgacgag ttcggcatcg aggtgctcca aggctgggga atgaccgaga cttcgcccat
     4801 cggcaccctg tccaacatga cgcccgaaac cctcgccctg ccgtatgacg agcagatgaa
     4861 atacagggtc aaacagggca ccccgccgct gggcgtcgag ctcaagctca agaactacgc
     4921 cggtcaggaa atgccccagg acgggcacac ctacgggcgg ctgatgatca agggcccgac
     4981 catcgccggc gcctatttcc gttcggacga aagcatcctc gatgcggagg gcttcttcga
     5041 caccggcgac gtgtcgacca tcgatgagat gggcttcatg cagatcaccg accgcgccaa
     5101 ggacgtgatc aagtccggcg gtgagtggat ctcatcaatc gagatcgaga acatcgccat
     5161 gggccacccc cgtgtcgaac tggccgcggt catcggcgcc gcccacccca aatgggacga
     5221 gcgcccggtg ctgatcgtca agctcaagcc cggcgagcct caggacaagc aggaacacct
     5281 cgacttcctc gtcggcaaga tcgccaaatg gtggatgccc gacgacgtgg tcttcgtcga
     5341 cgacatcccg ctcggcgcca ccggcaaggt cgacaagaag ctgatccgcg aacggctgaa
     5401 agactaccgc ctgccgacgg ctactgcggc atagcagtcc cctctccccg cgggagaggg
     5461 gacccagtcg tcagcttccg ttcacccttt cgcggttaat gctgatgtct cgggagaacc
     5521 agccatgacc aacgccctcc agcgcacatc taaactcgcc tgcgccatcg cggtgatcct
     5581 tggcggcagc gccatgctgc aaggctgcct cgtcggagcg gtcgcgggag caggcgtcgc
     5641 cgtggtcgga gggacggcca aggtcgtggg gggcgttgca ggcgcaggca tcgacgccgt
     5701 caccacttcc gatcaggaaa cccggcagcg gcgcgaacgt gaacagcgcg actacgagcg
     5761 tgagcaacgc cgctgcgagc agcgccaacg gcaaggcaag cgctgctagc atctggccca
     5821 gagcccgccg ccgcacgctt gcaccgaggc gaatacagtc acaattaccc gctagagcag
     5881 tggccgttgt gattccggag gttcgatatg atttcaattg ttatggcgac ggttctggct
     5941 ttccagtcgc cgacgcccgc caacaacagc actccgtccc gcgaagcccg gacctggcag
     6001 ggcgacacat ttcgttttat tttcttccct gacctgccgg gtggagcccc ggggagcgtc
     6061 agacttcgct gccgggtaag agacagtgga cgcctgatcg ggtgccgata cagcgaagcc
     6121 agcgatcacg aattcggagc ctatgccgtc cggtccgccc acaactcccg gatcgtcatg
     6181 gaggaaggcg ggccgcgagg tggggacttt atagaattcc ggttgatcag cgctggcgag
     6241 gatcaaacgc catagagcct cgaaacagcg ccccgacaga cctccgtcaa tgattctcta
     6301 gtctgtacgt aggatatatc ggtggtgatc cggggccgtc gcgtcatgct ggcgacatgt
     6361 gcagtttcgt ttccttccag acgccaaacc tcgccggaaa aggccgttga gcgccccccg
     6421 catcgcggtc atcggggccg gcttcagcgg cctcctgact gcggtccatc tgtcgcgacg
     6481 cctccccgac gccgagctct ggctgatcga aaagggctcc cgcttcgccg agggcgccgc
     6541 ctactcgacg cgagaggccg accacgtcct gaacgtccgt ctcggcaata tgagcgcctt
     6601 ccccgacgag cccgagcatc tgtcgcgctg gctcgccacc cagccccact ggtcttccaa
     6661 gggcgccttc atcacgcgcg gcgactatgg ccggtatctc agatcgatcc tgtccgacgc
     6721 cctttcaggt gctcgctctg aaagcgacag cgaagccggt tcacaaaccc gctcagccgc
     6781 cggctcccgc ctgaagctgg tgcacgacga ggtgctcgac ctcacccgcg ctggcggcgg
     6841 ctggcaggtc ctcctcggct ccggcgaaac gctggctgtc gacgtcgccg tcctcgccct
     6901 cggcaatctg gagcccgcac gcccgtccgg acttgacgag gctgtggtcg ccgccggtcg
     6961 ctacatcgcc gatccctggc gcggcgccgc caacctgccc gacgacgcgc gccgcatcct
     7021 cctgatcggc tccagcctga ccatggtcga cgtcgccctg tcccacgccc gccccggtcg
     7081 gcgctttttc gccctgtcgc gacgcggtct ggcgccccgc gcgcacaagg ccgccgatct
     7141 cgccctcacc tcacggccct actccggctc accgctgacg gtcctgcgtc aggcccgtcg
     7201 cgccgccctc acctccgact ggcgcgcggt cattgatgac cttcgccggt ccgcccgcac
     7261 tctgtggcgc tcatggtccc gagcccagcg cggtcgcttc ctgcgccatc tgcggccgat
     7321 ctgggacgtc caccgccacc gcctcgcccc cgccgtggcc agccgcatac agtcgatgat
     7381 cgcctcgggc gagctctcca tcgaggctgg ccgcatcgaa cggatcgagc ccgccggcac
     7441 caaggtcctc gtgacctggc gccggcgtgg ccggggtcgc accacgaccc gcaaggtcga
     7501 tgtcgtcatc aactgcaccg gccccctcgc cgacgtggtc gccagccaga tcccgctggt
     7561 gaagggactg gtcgcctccg gatacggccg cgcagatccc gacggtctcg gcctcgacgt
     7621 cgatgaacaa tgccgcgtcc gcgacatcga cggcgccccg caggacgaac tctatgccgt
     7681 cggtcccatg acccgaggcg ccttctggga agtcacctcc gtccccgaca tccgcatcca
     7741 ggccgccgaa gtcgccgaag tcatcgccta cagcgcgggc taaggccaag gtggccgagg
     7801 gttctcattc ccgacgcatc gaacgccgcc ctaaagcgtc agcgtgcact tctcatggat
     7861 ggtcgggcgg gcgaccgtga tgcgcgacag gccgggccag tcgggtttca gctgcgccac
     7921 aaaccacagg cacagatgct ccagcgtcgg cagttcgaga cccggctttt cgttcagcag
     7981 gccgtggtcc agttgctcgg ctgcggcttt cagcgcccga tcaagcgcgc caaggtccgc
     8041 aacccagccg tggccgtcca gcgtcgtgcc cttgacggtc gcctcgacct ggaaagagtg
     8101 gccgtgcatc cggcgataga tgctgccctc gggctcattg gggaaatggt gggccgcatc
     8161 gaaggtcgcg gctttcgtaa tctcgaactc ggtcatcgca cgccaacata cttgtgggtc
     8221 tgaacgctga gccgccacat cgggtgggtc aggcagtatt cgatcgccgc ctcggtattg
     8281 gccacctgat cggggccatc catcggctgc agccagaagc gctcgaaatc gagtccttcg
     8341 aagcggtcgg gcatggccga gccttgcggg aagaccagct tcagctcggc gccgcgcgtc
     8401 tgcagaaccg gcgcatcggc cttggg
//
LOCUS       DASVTM010000082         3865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_62002, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000082 DASVTM010000000
VERSION     DASVTM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62002"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF633_02255"
     CDS             <1..380
                     /locus_tag="VF633_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX8469910.1"
                     /translation="LTLRRGELLLDPGEFYILASSDAVEIPVDQAAEMTPIDPSVGEF
                     RVHYAGFFDPGFGTDEAHGAGSKGVLEVRTHDTPFLLEHGQIVARLVYEPLTERPSRL
                     YGEGGSHYQSQGLKLSKHFKEWV"
     gene            386..1006
                     /locus_tag="VF633_02260"
     CDS             386..1006
                     /locus_tag="VF633_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469911.1"
                     /translation="MRQLWPFTASIAALILATGACSREPSSDAASVPGSPETTAPAAP
                     AIQPTPVSVPEAAPATDASSKVLALEGLGALRIGQPVPNGGSWASRGAQISDACRTVT
                     SPDYPGIYAIVTDGEVRRITVGQRSDIKLIDGIGVGATEQAVNGAFPGFRSEPHQYEA
                     APAKYLTAPNAASGDPALRFEIGADGKVSLMHVGTMPVLGYVEGCS"
     gene            1119..1817
                     /locus_tag="VF633_02265"
     CDS             1119..1817
                     /locus_tag="VF633_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX8469912.1"
                     /translation="MDLGSVRDALAPILGAVVAGGIIGFEREWRGRPAGFRTHILVSL
                     TSALLMLAAMSQADWAFEALPNENIVTDPTRMAHGVLTGIGFLCAGVIFRNGFSIHGL
                     TTAASLWITAAIGLLFGAGLWELGVIGTAVTALILVGLRLVSARLPMRPLVDVVVCWT
                     RGPASPEAAVEALLQDVDVAVKNDGVALVEAGSMIRRRWRLKLTNPGQLTELARDLAR
                     LDGVVSFDLDPRDD"
     gene            complement(1828..3114)
                     /locus_tag="VF633_02270"
     CDS             complement(1828..3114)
                     /locus_tag="VF633_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8469913.1"
                     /translation="MTTETIETTEPVQPPPPPEGPASPWGIRDFKLLWFGRVVAVLAI
                     QIQSSALLWQVYTIARRDHPVAEASLYLGLVGLCQFLPLLALTLPAGAMADVRDRKIT
                     VAISIMVEGLCAFAFLMMALHGSPPLWGLLSVAAVFGAARAFLAPASQAFLPMVVGRR
                     ALPPAIAAQSVAFQTGAIAGPALGGVIVGVNVPLAYAVALGLFLLGVAAFLLIRTSGK
                     PAPNPNKLSPVESVKEGLSYVWKTKIVLGAISLDLVVVLLAGVALLTPIFARDILHVG
                     PEGFGLLRASFGVGALGMAVYLSRYPITKHGGLWMFSAVAVFGVCTLTFGLSKIVWVS
                     GLALLVGGAADMISVNVRQTLIQLATPDHMRGRVSSVSMLFIGASNELGEAYSGVMVR
                     ILGAVGAAVFGGVGALAATGIWSWMFPSLRKADKLS"
     gene            3211..>3865
                     /gene="dapE"
                     /locus_tag="VF633_02275"
     CDS             3211..>3865
                     /gene="dapE"
                     /locus_tag="VF633_02275"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX8469914.1"
                     /translation="MSHASNHVIDPVELTRRLIRRPSVTPADAGAMEVLETVLTELGF
                     TCRRMPFEGPGGEGVHARIENLYARRGTASPNLCFAGHTDVVPTGADSGWSSDPFQAE
                     VKDGILFGRGAVDMKGGIAAWVSAVAEVLSKGEPDGSLSFLITGDEEGPALHGTKRVV
                     ETLMAEGEVIDACVVGEPSSQGLLGDMIKIGRRGSLNTWITVHGKQGHVAYPQRAANP
                     "
BASE COUNT          667 a         1343 c         1267 g          588 t
ORIGIN      
        1 ctctgaccct tcgtcgtggc gagctgctgc tggacccggg cgagttctac atcctcgcct
       61 cgtccgacgc cgtcgagatc cccgtcgatc aggccgccga aatgaccccg atcgacccct
      121 cagtcggcga gttccgcgtc cactatgccg ggttcttcga ccccggtttc ggcacggacg
      181 aggcccacgg cgcaggctcc aagggcgtgc tcgaggtccg cacccacgat acccccttcc
      241 tgctggaaca cggccagatc gtcgcccggc tggtatacga acccctcaca gaacgccctt
      301 cacgcctcta cggcgaaggc ggcagccact accagagcca gggcctgaag ctctccaaac
      361 acttcaagga atgggtatag cctgcatgag acagctttgg ccgttcaccg cgtcgatcgc
      421 ggccttgatc ctcgccacag gcgcctgctc gcgtgagccg tcctccgacg ccgcctccgt
      481 tccggggtca cctgagacca cagcgcccgc cgcgccggcg atccagccaa ccccggtctc
      541 tgtgccggaa gccgcgcccg ctaccgatgc gtcctccaaa gtcctcgccc tcgaaggtct
      601 cggcgcgctc cgcatcggcc agccggtccc caacggcggc tcatgggcct cgcgtggcgc
      661 ccagatcagc gacgcgtgcc gcacagtgac ctcgcccgac taccctggca tctacgccat
      721 cgtcaccgac ggcgaggtcc gccgcatcac agtcggccaa cgctccgaca tcaaactgat
      781 cgatgggatc ggcgtcggcg ccaccgaaca ggccgtcaat ggcgccttcc ccggtttccg
      841 ctccgagccc catcaatacg aagccgcccc cgccaaatac ctcaccgccc ccaatgccgc
      901 ctccggcgac cccgccctcc gattcgagat cggcgcggac ggcaaggtca gcctcatgca
      961 cgtcgggacc atgccggtgc tggggtatgt ggaggggtgc agctaagtct gatcccatgg
     1021 ccggaccttg ggagcggtct gcgcaaccgc agcttttgac ggtgcgttaa agcaaaccgc
     1081 cgagccaggc gaggggcgga tcaggagcag cagcagccat ggacctcgga tctgtcagag
     1141 atgcgctggc gcccatcctc ggcgcggttg tcgccggcgg cataatcggg tttgagcgcg
     1201 aatggcgcgg tcgccccgca ggcttccgga cgcatatcct cgtcagtctc acttcggccc
     1261 tgctgatgct tgcggccatg tcccaggccg actgggcttt cgaggccctt cccaacgaaa
     1321 atatcgtgac cgatcccacg cgcatggccc acggcgtcct gacgggcatc ggcttcctgt
     1381 gcgcaggcgt gatcttccga aacggcttct ccatccacgg cctgacgacg gctgcctccc
     1441 tgtggatcac ggccgccatc ggcctcctgt tcggggctgg cctttgggag ctaggcgtca
     1501 tcggaaccgc cgtgacagcg ctgatcctcg ttggactgcg gctggtcagc gcgcggttgc
     1561 cgatgcggcc cctcgtcgat gtggtcgtct gctggacgcg aggtccggcc tcgcccgagg
     1621 cggcagtgga ggccctgctt caggacgttg acgtcgcggt caagaatgac ggcgtcgctc
     1681 tggtcgaggc ggggtcaatg atccggcgcc gatggcggtt gaaactcacc aatcccgggc
     1741 agttgaccga gctggcccgg gatctggccc gactggacgg agtggtgagt ttcgacctcg
     1801 atccgcgcga cgattgaccg cgcgtcgtca gctcaacttg tcggccttcc gcaagctcgg
     1861 gaacatccag ctccagatgc cggtcgcggc cagcgcgccg acgccgccga acaccgccgc
     1921 tccgaccgcg cccagaatgc ggaccatgac gcctgaatag gcctcgccga gttcgttgga
     1981 ggcgccgatg aacagcatgg agaccgacga cacccggccg cgcatgtggt cgggggtggc
     2041 gagctggatc agggtctggc ggacgttgac gctgatcatg tcggcggcgc cgcccaccag
     2101 cagggcgagg cccgagaccc agacgatctt cgacaggccg aacgtcaggg tgcagacgcc
     2161 gaacacggcg acggccgaga acatccacag tccgccgtgt ttggtgatcg gatagcggct
     2221 cagatagacc gccatgccca gggcgccgac gccgaacgag gcgcgcaaca ggccgaagcc
     2281 ctcgggcccg acgtgcagga tgtcgcgggc gaagatcggc gtcagcagcg cgaccccggc
     2341 cagcagcacc accaccagat ccagcgagat ggcgcccagc acgatcttgg tcttccagac
     2401 atagctcagg ccttccttta cggattcgac cggcgacagc ttgttggggt ttggcgcggg
     2461 cttgccgctg gtgcggatca gcaggaaggc ggcgacgccc agcaggaaaa gccccagcgc
     2521 cacggcatag gccagcggta cgttgacgcc gacgatgacc ccgccgagcg caggaccggc
     2581 gatggctccg gtctggaagg cgacggactg ggcggcgatg gcgggcggca gggccctgcg
     2641 accgaccacc atcggcaaaa aggcctggct ggcgggagcg aggaaggcgc gggcggcccc
     2701 gaacacggcg gcgaccgaca gcagccccca cagcggcggt gagccgtgca gcgccatcat
     2761 cagaaaagcg aaggcgcaca agccttcgac catgatcgag atggcgacgg tgatcttgcg
     2821 gtcgcgcacg tcggccatgg cccctgcggg cagggtcagg gccagcagcg gcaggaattg
     2881 gcacaggccg acgaggccga gatacaggct ggcctctgcc accggatggt cgcgccgcgc
     2941 gatcgtatag acctgccaca gcagggccga ggactggatc tggatcgcca gcaccgccac
     3001 cacgcgtccg aaccacagca gtttgaagtc ccggataccc cacgggctcg ccggaccctc
     3061 aggcggaggc ggcggctgga cgggttcggt ggtctcgatg gtttctgtgg tcacggtcgg
     3121 gacgggtctc ggacagggga ggagggcaag atcaaagccg ctgggctggc cccttctcgc
     3181 aatcgcgtaa cgtccctaaa gcataggcgc atgagccacg catcaaatca cgtgatcgat
     3241 cctgtcgaac tgacccgccg cctgatccgc agaccatcgg tcacgcccgc cgacgccggc
     3301 gcgatggagg tgctggagac ggtgctgacc gaactcggct tcacctgccg ccggatgccg
     3361 ttcgagggac cgggcgggga gggcgtccat gcccggatcg agaacctcta cgcccggcgc
     3421 gggaccgcct cgcccaacct ttgtttcgcc ggtcataccg acgtggtgcc gacgggcgcc
     3481 gacagcggct ggtcgtcgga tcccttccaa gccgaggtca aggacggcat cctgttcgga
     3541 cgcggcgccg tcgacatgaa gggtggcatc gccgcgtggg tctcggccgt ggcggaggtt
     3601 ttgtccaaag gcgaacccga cggctccctg tccttcctga tcaccggcga cgaagagggt
     3661 ccggccctgc acggcaccaa gcgcgtcgtc gaaaccctga tggccgaggg cgaggtcatc
     3721 gacgcctgcg tggtcggcga accgtcgtcg caagggctgc tcggcgacat gatcaagatc
     3781 ggtcgtcgcg gctcgctcaa cacctggatc accgttcacg gcaaacaggg ccacgtcgcc
     3841 tatccccagc gcgccgccaa ccccg
//
LOCUS       DASVTM010000083         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_167787,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000083 DASVTM010000000
VERSION     DASVTM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167787"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VF633_02280"
     CDS             <1..1602
                     /locus_tag="VF633_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX8469915.1"
                     /translation="ARDILVRQGLTAESVPEAGRFVMLRTTANLSSGGTAVDRTDEIH
                     PDNASIARRAALTLGLDVAGIDLLAPDITRSVRETGGGIVEVNAAPGLRMHLDPSEGR
                     ARDVAGPILRMMYPPGVRARIPVLAVTGTNGKSTVGRMLALIFTCEGQTVGLTNTSGV
                     YIGDEQVFAGDASGPKSARLVLRDPTVDVAVLECARGGILREGLAFDRCDVGAVLNVQ
                     PDHLGLKGIETLEDLAAVKSVVIETVSRRGVSVLNADDPLTVRMARHAGGRLCWFSMR
                     GGSDTPELVRRHIADGGLAALFNPADGGLTLYDDGVVHPVCRAVDIPATLGGHAAFNI
                     ANALAAAAMAYGAGIEPVRIARALSGFASTYEQNPGRLNVHDAHGVRIIMDYAHNPEG
                     LRALGRLIQAARPDSGRTIAMLSVPGDRRDAEVTAMGAIGAEFFDELVFRETPDNRGR
                     RAGEVIRLLTEGAVSAGCDLSRIRGVIREEDPVAECLAMARPGDMVILTPTRIDSVWR
                     QVLDYSLGPARPLSPAEAILEPAHG"
     gene            1595..>2372
                     /locus_tag="VF633_02285"
     CDS             1595..>2372
                     /locus_tag="VF633_02285"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX8469916.1"
                     /translation="MANAGPLIIIGGHEDKTDERVILKAVAEHVNGGKLVIATVASHQ
                     PEGYFDSYRKGFEGMGVGELVELYVHERSESQADAALAILDGAAGVFFSGGDQLRISS
                     QIGDTPVERRIREMHAAGAVIAGTSAGASVMSETMLVKGASAESYRIGELHMAPGLGL
                     VRDVIIDQHFAERGRYGRLLGAVAHNPRLLGIGIDENTAIKVEGRSFRVLGSGAAYVV
                     DGEGSTHSNIAEAKSERALSMFDVRMHVLSSGDRFDLDARK"
BASE COUNT          393 a          755 c          794 g          430 t
ORIGIN      
        1 gcgcgcgata tattggtccg tcagggcctg acggctgaaa gcgtgcctga ggcgggacgg
       61 ttcgttatgc ttcggacgac cgcgaacctc tcgtccggtg gcacggcggt tgatcgcacg
      121 gatgagatcc accctgacaa cgcgtccata gcccgccgcg ccgccctgac gctcggtctg
      181 gatgtcgcgg ggatcgatct tctggcgccg gacatcaccc gttcagttcg cgagaccggg
      241 ggcggcatcg tcgaggtcaa tgccgcgcct ggccttcgga tgcacctgga tccatcggaa
      301 ggccgggctc gggacgtggc tggccccatt ctgcgcatga tgtatccccc cggcgtccgg
      361 gcccggattc ccgtgctggc ggtcaccggc acgaacggaa aatcgacggt gggccggatg
      421 ctcgccctca tcttcacgtg cgagggccag accgtcggcc taaccaacac cagcggcgtc
      481 tatatcggcg atgaacaggt ctttgcgggc gacgccagtg gaccgaagag cgctcggctc
      541 gtgctgcgcg accctaccgt ggacgtggcc gtactcgaat gcgctcgagg cggcatcctc
      601 agggagggcc tcgccttcga ccgctgcgat gtaggcgcgg tgttgaacgt tcagcccgac
      661 catctgggtc tgaaggggat cgagacgctt gaggatttgg cagccgtcaa gtcggtcgtg
      721 atcgagacgg tgtcgcgtcg cggtgtcagc gtcctgaacg ccgacgaccc gttgacagtg
      781 cggatggcgc ggcacgccgg gggacggctc tgctggttct cgatgcgcgg cggctccgac
      841 actcccgaac ttgttcggcg ccatatcgcg gacgggggac ttgccgccct gttcaatcct
      901 gcagatggcg gtttgaccct gtacgacgac ggcgtggtcc atcccgtttg ccgagcggtc
      961 gatatcccgg cgaccctggg cggccatgcc gcattcaaca tcgccaacgc gctggcggct
     1021 gccgcgatgg cctacggagc aggcatcgag cctgtccgga tcgctagagc cctgtctggc
     1081 ttcgcctcga cctatgagca aaatcccggt cgactcaacg ttcacgacgc ccacggcgtt
     1141 cggatcatta tggactacgc gcataatccc gaaggcttgc gcgccctggg acgtctgatc
     1201 caggccgccc gccccgactc gggacgcacc atcgccatgc tcagcgtgcc tggagaccgc
     1261 cgggatgccg aggtcacggc gatgggagcg atcggggccg aatttttcga cgagctcgtc
     1321 tttcgcgaaa ctccggacaa ccgggggcgg cgggccggcg aagtgatccg gctgctgaca
     1381 gagggcgccg tgagcgcggg ctgcgatctg tcgcgcattc gcggcgtcat ccgcgaggag
     1441 gatccagtgg ctgaatgcct ggccatggcc aggcccggcg acatggtgat cctgacccct
     1501 acgcggatcg attccgtctg gcgtcaggtc ctcgactatt cccttggacc ggcgcgtcct
     1561 ctttcgccgg ctgaagcgat cctggagcct gcccatggct aacgccggac ccctcatcat
     1621 tatcggcggg cacgaggaca agaccgacga acgggtcatc ctcaaagcgg tggccgaaca
     1681 cgtcaacggc gggaagcttg tcatcgccac cgtcgcttcc caccagccgg aaggctattt
     1741 cgacagctac cgcaagggct tcgagggtat gggcgtcggc gaactggtcg agttgtacgt
     1801 ccacgaacgc tccgaaagcc aagctgatgc ggcgctggcc attcttgacg gcgcggccgg
     1861 cgtgtttttc agcggcggcg accagttgcg aatttccagc cagatcggcg ataccccggt
     1921 cgagcgccgg atccgcgaaa tgcatgccgc gggcgctgtc attgccggta cgtctgcggg
     1981 cgcttctgtc atgagcgaga ccatgctggt caagggagcc agcgccgagt cgtaccggat
     2041 tggcgaactc catatggccc ccggtctcgg tcttgtacgg gacgtgatca tcgatcagca
     2101 ttttgccgaa cgcgggcgat atggccgcct gcttggcgcg gtcgcccaca acccgcgtct
     2161 gctcggtatc ggcatcgatg agaacacagc catcaaggtc gagggtcgaa gcttcagggt
     2221 gctgggcagc ggggcggcct atgtcgtgga tggcgagggc tccacccatt caaatatcgc
     2281 cgaggcgaag tcggaacggg ccctctcgat gttcgacgtg cggatgcatg tgctgtcgag
     2341 cggagaccgg ttcgacctgg acgcccgcaa gc
//
LOCUS       DASVTM010000084         4675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_326637,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000084 DASVTM010000000
VERSION     DASVTM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326637"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /gene="hutH"
                     /locus_tag="VF633_02290"
     CDS             complement(<1..1172)
                     /gene="hutH"
                     /locus_tag="VF633_02290"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX8469917.1"
                     /translation="MTHLTLGGAPLTLPQLRAVLDAPCTVELTPTAWVCVEKSAATIA
                     AIVAEGRTVYGVNTGFGLLANTSIAPGDLETLQRNLVLSHACGVGPLLPDPVTRLMMV
                     LKIASLSRGASGVRRETLEALIALVNADVLPCVPSKGSVGASGDLAPLAHMSCVLIGV
                     GEARHRGQTLDAEAALAVAGLTPLTLGPKEGLALLNGTQCSTALALAGLFATEAVFAA
                     GIAAGALSVDALKGSDAPFDPRIHALRGQPGQIAVAATLRHIMSGSAIRDSHLDDCSK
                     VQDPYSLRCQPQVMGAILDVLTASAAMLEREANAVTDNPLVFIEEGDVISGGNFHAEP
                     VAFAADQIAMALCEIGNISERRTAILVDPKMSELPAFLVRESGLNSGFMIAQVTAAA"
     gene            complement(1175..2845)
                     /gene="hutU"
                     /locus_tag="VF633_02295"
     CDS             complement(1175..2845)
                     /gene="hutU"
                     /locus_tag="VF633_02295"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX8469918.1"
                     /translation="MTDKTPNARVIRSPRGPDKTAKTWAAEAAMRMLMNNLDPEVAER
                     PDDLVVYGGIGKAARDWASFDRIVAELQTLEADQTLLVQSGKPVGVFRTHEDAPRVLI
                     ANSNLVPKWATWEHFNELDRKGLMMYGQMTAGSWIYIGTQGIVQGTYETFMEAGRQHY
                     GGEWTGRWILTAGLGGMGGAQPLAAVMAGAHCIAVESQESSIEKRLETRYLDVMAETL
                     DEALALIEDAGRTGKPVSVGLLANAADVLPELVRRGVRPDLVTDQTSAHDLINGYLPA
                     GWTVAEWEDKRLSDPASVEAAAKTSVVAHVQAMLDFQSAGVPVVDYGNNIRQVAFDAG
                     VAAAFDFPGFVPAYIRPLFCRGIGPFRWVALSGDPADIAKTDAAMNRLFPDDAGLHRW
                     LDMAGESIAFQGLPARICWIGLGDRHRAGLMFNEMVASGEISAPIVIGRDHLDSGSVA
                     SPNRETESMIDGSDAVSDWPLLNALLNTASGASWVSIHHGGGVGMGYSQHAGVVIVAD
                     GTPEAARRLERVLWNDPATGVMRHADAGYDIARDAAKAHGLNLPSLKA"
     gene            complement(2842..3663)
                     /gene="hutG"
                     /locus_tag="VF633_02300"
     CDS             complement(2842..3663)
                     /gene="hutG"
                     /locus_tag="VF633_02300"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEX8469919.1"
                     /translation="MKDWLTVREGAAPLIIGFPHTGTEIPAAIEARMTSPWLARKDAD
                     WWVDRLYDFAVELDATFVRTAISRSVIDVNRDPSGASLYPGMTTTGLCPIETFDGEPL
                     YRAGQAPDRAEIDARRAAFFDPYHAALQAQIDRLSTNGPVVLYDAHSIRSVVPRLFEG
                     ELPQFNIGDNGGTTCDGNLTRVVEAACAASGSSHIVNGRFRGGWTTRHYGKPQDGLHA
                     IQMELADRGYMLDPAGPVSEANWPSPYEPARAEPMRETLRAVLTACIDFARSSTA"
     gene            3741..>4675
                     /gene="hutF"
                     /locus_tag="VF633_02305"
     CDS             3741..>4675
                     /gene="hutF"
                     /locus_tag="VF633_02305"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050416 - formimidoylglutamate deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX8469920.1"
                     /translation="MPLTSSEGRSLWFEQALLGEVWARDVRITVVDGLISAVETGVAR
                     GPSDNGGGVAMPGVANVHSHAFQRAMAGLTEGRGPEGGDDDDFWSWRQLMYRFLDRGG
                     PDEIEAITALAFAEMLEGGFTRLAEFHYLHNDPSGAAYADPGELTGRIVAAAEATGVG
                     LTLLPVFYAHAGFGGQPPSPGQRRFITGLDGFADLVARGRTLTAGLEDAVVGVAPHSL
                     RAVSPDELAVMPGLAPDGPIHIHVAEQTREVVDCLAWSGARPVEWLLANAPVDPRWCL
                     IHATHVTEPEWREVAARGAVVGLCPVTEANLGDGV"
BASE COUNT          731 a         1463 c         1709 g          772 t
ORIGIN      
        1 gcggcagccg tgacctgggc gatcatgaag cccgagttga gcccgctctc acgcacgagg
       61 aaggccggaa gctcgctcat cttgggatcg acgaggatgg cggtgcggcg ctcggagatg
      121 ttgccgatct cgcacagggc catggcgatc tggtcggcgg cgaaggccac gggctcggcg
      181 tggaagttgc cgcccgagat gacgtcgccc tcttcgatga acaccagcgg gttgtcggtg
      241 acggcgttcg cctcgcgctc cagcatggcg gcggaggcgg tcagcacgtc aaggatcgcg
      301 cccatcacct gaggctggca gcgcagggag taggggtcct gcaccttgga gcagtcgtcg
      361 agatggctgt cgcggatggc gctgccggac atgatatggc gcagggtcgc ggcgacggcg
      421 atctggccgg gttggccgcg cagggcatgg atgcgcgggt cgaacggggc gtcggagccc
      481 ttgagcgcgt ccaccgacag ggcgccggcg gcgatcccgg cggcgaagac ggcctcggtc
      541 gcgaacaggc cggcgagtgc cagcgcggtt gaacactggg tgccgttgag cagggccaga
      601 ccttccttgg ggccgagcgt cagaggggtc agcccggcca cggccagcgc cgcctcggcg
      661 tccagcgtct gaccgcgatg ccgggcctcg ccgacaccga tcaggacgca ggacatgtgg
      721 gcaaggggcg cgaggtcgcc cgaggcgccg acggagccct tggatgggac gcagggcagg
      781 acgtcggcgt tcaccagcgc gatcagggct tcgagggtct cgcggcgaac gccggatgcc
      841 ccgcgcgaca ggctggcgat cttgagcacc atcatcagcc gggtgaccgg gtccggtagc
      901 agggggccga caccgcaggc atggctgagg acgaggttgc gctgcagggt ctccagatca
      961 ccgggcgcga tcgaggtgtt ggccagcagg ccgaagccgg tgttgacgcc atagacggtg
     1021 cggccctcgg cgacgatggc ggcgatggtc gcggcgctct tctcgacgca gacccacgcc
     1081 gtcggggtca gctcgacggt gcagggcgcg tcgagcacgg cgcggagttg gggcagggtg
     1141 agcggggcgc cgccgagggt gaggtgggtc atggtcaggc cttcaggctc ggcaggttga
     1201 gtccgtgcgc tttcgccgcg tccctggcga tgtcgtagcc ggcgtcggcg tggcgcatga
     1261 cgccggtggc ggggtcgttc cagaggacac gttcgagacg cctcgcagcc tcgggcgtac
     1321 cgtcggccac gatgacgacg cccgcgtgtt gcgagtagcc catgcccact ccgccgccgt
     1381 gatggatcga cacccagctg gctccggagg cggtgttcag gagagcgttc agtagcggcc
     1441 agtccgacac cgcatctgag ccgtcaatca ttgattccgt ctcgcgattg gggctggcga
     1501 ccgagcccga gtcgaggtgg tcgcggccaa tcacgatcgg cgcggagatt tcgcccgagg
     1561 ccaccatctc gttgaacatc agcccggcgc ggtggcggtc acctaatccg atccagcaaa
     1621 tacgcgccgg aagtccttgg aaagcgatgc tttcgcccgc catatctagc cagcgatgta
     1681 gtccggcatc atctggaaaa agtcggttca tggcggcgtc ggtcttggcg atatcggcag
     1741 gatcgccact cagcgccacc caccggaacg ggcctatgcc gcgacagaac aacggtctga
     1801 tgtaggccgg gacgaagccg ggaaagtcga aggcggcggc cactccggcg tcgaatgcga
     1861 cctgtcgaat gttgttgccg tagtcaacca caggcacgcc tgccgactgg aaatcgagca
     1921 tggcctggac gtgggcgacg acggaggtct tggccgcagc ttcgaccgag gcggggtcgg
     1981 acaggcgttt gtcctcccat tccgcaactg tccagccggc gggcagataa ccattaatca
     2041 ggtcgtgggc gctggtctgg tcggtgacga gatcgggtcg cacgccgcgc cggaccagtt
     2101 caggcagcac gtcggcggcg ttggcgagga ggccgaccga gacgggtttg ccggttcggc
     2161 ccgcatcttc gatcagggcg agagcttcat ccaaagtctc agccatgacg tcgaggtagc
     2221 gggtctcaag acgtttctcg atcgaactct cctggctctc gaccgcgatg cagtgcgccc
     2281 cggccatcac cgccgcgagc ggctgcgcgc cgcccatgcc gccaaggccc gcggtcagga
     2341 tccaccggcc cgtccactcg ccgccatagt gctggcgacc ggcctccatg aaggtctcgt
     2401 aggtaccctg aacgatgcct tgcgtgccga tgtagatcca ggacccggcg gtcatctggc
     2461 cgtacatcat cagccccttg cgatcgagct cgttgaaatg ctcccaggtc gcccatttcg
     2521 gcacgaggtt ggagttggcg attagcacgc gcggggcgtc ctcgtgggtg cggaacacgc
     2581 cgaccggctt gcccgattgc accagcagcg tctggtcggc ctcgagagtc tggagctcag
     2641 caacgatgcg gtcgaaactg gcccagtcgc gcgccgcctt accgatgccg ccatagacga
     2701 cgaggtcgtc cgggcgctcg gccacctcgg gatcgaggtt gttcatcagc atccgcatcg
     2761 ccgcctcggc ggcccaggtt ttggcggtct tgtccggtcc gcgcgggcta cgaatgaccc
     2821 gagcattggg agtcttgtcg gtcatgctgt ggagctccgg gcgaagtcga tacaggcggt
     2881 caggacggcg cgcagggttt cgcgcatcgg ctcggctcgc gcaggttcgt agggggacgg
     2941 ccagttcgcc tcgctgacgg gaccggccgg atcgagcatg tagccgcgat cggcgagctc
     3001 catctggatg gcatgcaaac cgtcctgtgg tttgccatag tggcgcgtgg tccagccgcc
     3061 ccggaagcgg ccgttgacga tatggctgct gcccgaggcg gcgcaagctg cctcgaccac
     3121 gcgggtcaga ttgccgtcgc aggtggtccc gccgttgtcg ccgatgttga actgcggcag
     3181 ttcgccctcg aacagacgcg gcacgaccga acggatcgaa tgggcgtcat agaggacgac
     3241 ggggccgttg gtgctcagcc ggtcgatctg ggcctgaagc gctgcatggt aggggtcgaa
     3301 gaaggccgct cggcgcgcgt cgatctcggc gcgatccggc gcctggccgg cccgatacag
     3361 cggttcgccg tcgaaggtct cgatcgggca caggccggtg gtggtcatgc ccggatagag
     3421 ggaggcgccc gacggatcgc ggttgacgtc gatgacgctg cgcgagatcg cggtgcgcac
     3481 aaaggtcgcg tcgagctcga ccgcaaagtc gtatagccgg tccacccacc agtcggcgtc
     3541 cttgcgggcc agccagggcg aggtcatccg ggcctcgatc gccgccggga tctcggttcc
     3601 cgtgtgcgga aatccgatga tcaggggcgc ggcgccttcg cgcacggtca gccagtcttt
     3661 catcgttgag gcctccctcg ccattccgga tcaacgtggt atgtctagac atattgttcg
     3721 aagccgaccg ggattgtcca gtgcctctaa cttcgtctga aggtcgcagc ctgtggttcg
     3781 aacaggcgct gctgggggag gtctgggcgc gcgacgtgcg gatcacggtc gtggacgggt
     3841 tgatatcggc ggtcgagacc ggggtggcgc gcgggccgtc ggataacggc ggcggggtcg
     3901 ccatgccggg cgtcgcgaat gttcacagcc atgcgttcca acgcgccatg gccggtctga
     3961 ccgaaggacg cggacccgag ggcggggacg atgacgactt ctggagctgg cgacagctga
     4021 tgtaccggtt cctcgaccgg ggcggcccgg acgagatcga ggccattacg gcgttggcct
     4081 ttgccgagat gctggagggc gggttcaccc gcctcgctga gttccactat ctgcacaacg
     4141 acccgtcggg agcggcttac gccgaccccg gcgagctgac cggacggatc gtggcggcgg
     4201 cggaggcgac gggcgtaggc ctgacgctgc tgccagtgtt ttacgcccat gccgggttcg
     4261 gtggtcagcc gccgtcgccg ggccagaggc ggttcatcac cggtctcgat gggttcgccg
     4321 atctggtggc gcgcggccgg acgctgacgg cgggactcga ggacgcggtg gtcggtgtcg
     4381 cgccgcacag cctgcgcgcc gtctcgccgg atgaactggc cgtgatgccg ggactggcgc
     4441 cggacggacc gatccacatc cacgtcgccg aacagaccag ggaggtcgtc gactgcctcg
     4501 cctggtcggg tgcgcggccc gtcgaatggc tgctggccaa tgcgccggtc gatccgcgct
     4561 ggtgcctgat ccacgccacc cacgtcaccg aaccggagtg gcgcgaggtt gcggcgagag
     4621 gcgctgtggt tgggctgtgc cctgtgaccg aggccaatct cggcgacggc gtctt
//
LOCUS       DASVTM010000085         5437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1018417,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000085 DASVTM010000000
VERSION     DASVTM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018417"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF633_02310"
     CDS             <1..429
                     /locus_tag="VF633_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169719.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8469921.1"
                     /translation="AQYDYVQDQPPKLRGYQATNRVTVNINDLTKVGTTADAVVAAGV
                     NQIDGISFGLKDPKTAEDQARRQAVEALQSKAQLYAQALGVQLGGIRSLNEGGGYTPQ
                     PPMPVFAMARMQSAGADSTPVSAGELSVKIEITGVYDIAR"
     gene            565..1812
                     /locus_tag="VF633_02315"
     CDS             565..1812
                     /locus_tag="VF633_02315"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX8469922.1"
                     /translation="MIRIFGLAAISAALMASGAAAQATTDRAGEARVLDILKTTPLID
                     GHNDLPWALREKHGNDPYAVDLTTDLTATGLHTDIPRLRAGGVGAQLWSVYVPADIAP
                     VEAANETFEQIDTVRRIVAAHPDVFELATTADDIERIHRSGKIASLIGMEGGYSIDDS
                     LGLLREFYVAGARYMTLTHSKTTTWADSATDAPKWGGLNPFGEAVVHEMNRIGMMVDL
                     SHVSEETMLDAVRVSEAPVIFSHSSARAVTDHNRNVPDNVLRLLPENGGIVMVTFVPG
                     FVSVALPQWNRDLEAEAKRLGADPAAATPDPALVAWATAHPRPHATIADVVAHIHHVR
                     EVAGIDHVGLGGDFDGVPTLPNGITGVDAYPIILAALMQSGWTEADIRKISGENMLRV
                     MRAVEAVAAKKSNERPSLATNVL"
     gene            complement(1809..2324)
                     /locus_tag="VF633_02320"
     CDS             complement(1809..2324)
                     /locus_tag="VF633_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469923.1"
                     /translation="MGLLDFLTGGSKPATDRNRATTPTLREALLAINRDTSPFQIRDG
                     DPEGVDLVAEWKIVDAAWYEIFAKAGTKRVFKVLMKFDEDSGQVRSIDREFDVEWRAG
                     VPELSATVQAFRGQKWETSFEAVYAFDEKDLHWGKVYQYRFQTDEMKKPLKAAAEAAG
                     WGWRGVAFGKL"
     gene            complement(2397..2837)
                     /locus_tag="VF633_02325"
     CDS             complement(2397..2837)
                     /locus_tag="VF633_02325"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX8469924.1"
                     /translation="MRLALFQPDIPQNVGACIRLGACFGVELHVIEPVGFQFDDRAMK
                     RAALDYGPLGHMRRHSDWDAFQENRGPGRLVLFTTKGATSLSEFSFEADDMLLFGRET
                     VGVPDHVHAAANARVFIPIRPEARSLNLAVSAGIGLWEGLRQTR"
     gene            3034..3573
                     /gene="petA"
                     /locus_tag="VF633_02330"
     CDS             3034..3573
                     /gene="petA"
                     /locus_tag="VF633_02330"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX8469925.1"
                     /translation="MAEPVVTDGVETDATRRDFIHIAAGAMAVGAGAMVAWPLINQMN
                     PAADTLALSTIEFDVSKVQEGQQVVITWQGKPVFVRYRTAKEIQEANAASPGDLKDPE
                     TDAARVKPGHSQYLVVIGSCTHLGCIPTFGSGEFGGWFCPCHGSVYDTSGRIRKGPAP
                     TNLVVPEYAYLSDTRIKIG"
     gene            3588..4916
                     /locus_tag="VF633_02335"
     CDS             3588..4916
                     /locus_tag="VF633_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX8469926.1"
                     /translation="MSDHESTYVPQTGVEKWLDTRLPIIRFGADYLSLPTPKNLNYWY
                     TFGGILAVCLVIQIITGIVLAMHYTPNTAMAFASVERIMRDVNGGWLIRFIHANGASM
                     FFIAVYIHMLRGLYYGSYKAPREMIWILGCVIFFLMIATAFLGYVLPWGQMSYWGAEV
                     ITNLIGAIPLIGEPILIWLRGGPGIDNATLNRFFSLHYLLPFVIFGVVVLHLWALHTS
                     GQNNPVGILIPKDREKKDMVPFHPYYTVKDGFALLLFLILFSVFVFFMPDSLGHADNY
                     IQANPLQTPAHIVPEWYMLPFYAILRAVPDKFGGVVAMFGSIGVLFILPWLDTSKVRS
                     MRYRPTMRTFFLIFVFVGIGLGWCGGQLPDAHVIPGLSTFVFIDGDLNSYLWLTRLFT
                     AYYFAFFFIILPLVGLKETPLPIPASISEPVLTGSHAMSGAVPAQAEKKG"
     gene            4922..>5437
                     /locus_tag="VF633_02340"
     CDS             4922..>5437
                     /locus_tag="VF633_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX8469927.1"
                     /translation="MTFFKSKTLALRNTLAAITAVAGLAVMAAPASAEGGHHLRDGGF
                     TFEGPFGTFDQGQLQRGYKVYREVCASCHSMDLMHFRTLGQKGGPFYDEHAANPAENR
                     FVKALAAEVQVADIDNETGEAIQRPGIAADTFPSPYPNKTAAAAANGGAAPPDLSVMA
                     KARHGGANYIYS"
BASE COUNT          969 a         1813 c         1623 g         1032 t
ORIGIN      
        1 gcccagtacg attacgttca ggaccagccg cccaagctgc gcggctatca ggccaccaac
       61 cgcgtgacag tcaacatcaa cgatctgacc aaggtcggca cgaccgcaga cgccgtggtc
      121 gccgccgggg tcaaccagat cgacggcatc tcgttcggcc tcaaggatcc caagaccgcc
      181 gaggatcagg cgcgtcgtca agcggttgag gcgctgcaat ccaaggcgca gttgtacgcc
      241 caggcgctcg gggttcaact cggcggcatt cgttcgctga acgagggcgg cggttatacg
      301 ccccagcctc cgatgccggt cttcgccatg gcacggatgc agtccgccgg cgccgattcg
      361 acaccggtct cggcaggcga actgtcggtg aagatcgaga tcacaggcgt ctacgacatc
      421 gctcgctaag actgttgatc gacagggtac ggggtcgcag cgatgcggcc ccgttttcca
      481 tgtctgcgga cttacggcga tttcacttgt gaccggacgg cgttcggctg acttctcgga
      541 aacgcgttgt ctggagcctc ccgcatgatc cgcatctttg gcctcgccgc catatccgca
      601 gcgcttatgg cgtcaggcgc agcggcgcag gccacgacgg atcgcgcagg cgaggcgagg
      661 gtgctggaca tcctcaagac gacgccgctg atcgacggtc acaacgacct gccctgggcc
      721 ctgcgcgaga agcacggcaa cgatccctac gccgtcgacc tgaccaccga cctcacggcg
      781 acaggcttgc atacggatat tccccggcta cgggcgggcg gagtcggcgc gcaactctgg
      841 tcggtctatg tgccggctga catcgcgcct gtcgaagcgg ccaatgaaac cttcgagcag
      901 atcgacaccg tccgccgcat cgtcgccgcc catccggatg tgttcgaact agccaccacc
      961 gccgacgaca tcgagcgcat tcatcgctcc ggaaagatcg ccagcctgat cggcatggaa
     1021 ggcggctatt cgatcgacga ttctctgggc ctgctgcgcg agttctatgt cgccggcgcc
     1081 cgctatatga ctctgacgca ttcaaagacg accacgtggg ccgacagcgc caccgatgcg
     1141 ccgaaatggg gcggcctcaa tcctttcggc gaggcggtgg tccacgagat gaaccgcatc
     1201 ggcatgatgg tcgacctgtc ccacgtgtcc gaggagacga tgctggacgc cgtccgagtg
     1261 tcggaagccc cggtgatctt ttcgcactca tctgctcgag ccgtgacgga ccacaatcgc
     1321 aacgttcccg acaacgtcct gcgtcttctg cccgaaaacg gcggcatcgt catggtgacc
     1381 ttcgttccgg gcttcgtcag cgttgcgctg cctcagtgga accgagacct ggaagctgag
     1441 gctaaaaggc tgggtgctga ccccgcggcc gcgacgccgg acccggcact ggtggcctgg
     1501 gccaccgccc accccagacc tcatgccacg atcgctgatg tcgtcgccca tattcatcat
     1561 gtccgggagg tcgctggcat agatcacgtc ggtctcggcg gagacttcga cggcgtaccg
     1621 accctgccca atggcatcac cggtgtagac gcctatccca tcatccttgc cgcgctgatg
     1681 cagtccggtt ggactgaagc tgacatccgg aagatttctg gcgagaacat gctgcgggtt
     1741 atgcgagccg ttgaggcagt ggcggccaag aagtcgaacg agcgtccgtc gctggcgaca
     1801 aacgtgctct agagtttgcc gaacgcgacg ccgcgccaac cccagcctgc ggcctcggcc
     1861 gccgctttca gcggtttctt catctcgtcg gtctggaacc ggtactgata gaccttgccc
     1921 cagtgcaggt ccttttcgtc gaaggcgtag acggcctcga aactggtctc ccacttctgg
     1981 ccgcgaaaag cctgcactgt ggccgacagt tcaggcacgc ccgcccgcca ttcgacgtcg
     2041 aactcgcgat cgatcgagcg gacctggccg ctgtcctcgt cgaacttcat cagcaccttg
     2101 aacacgcgct tggtgccggc cttggcgaag atttcgtacc aggcggcgtc gacgatcttc
     2161 cactcggcga cgagatccac cccctcgggg tcaccatcgc ggatctggaa cggcgaggtg
     2221 tcacggttaa tggccagcag agcctcgcga agcgtcggcg tcgtcgcccg gttgcggtcg
     2281 gtcgcgggct tcgacccgcc cgtcaaaaaa tccagtagac ccattgccgt gcttccatcc
     2341 ctgcagacgc tgtgggcacc ctgacagatc aaacccggcc gggcgagcgt cgttcttcaa
     2401 cgtgtctgcc gcagaccttc ccacaggcca atcccggcgc tgaccgccag gttcagagag
     2461 cgggcctcgg gccgaatcgg aatgaacacc cgcgcattgg cggcggcatg gacatgatct
     2521 ggaacaccga cggtctcgcg accgaacagc agcatatcgt cggcctcgaa gctgaactcc
     2581 gaaaggctgg tcgcgccctt ggtcgtgaac aaaaccagcc tgccgggacc gcgattttcc
     2641 tgaaaggcgt cccagtccga atgccgtctc atatgaccca gaggtccata gtctagcgca
     2701 gcgcgcttca tggcccggtc gtcgaactgg aagccgacgg gctcgatgac gtgcagttcc
     2761 acgccgaaac aggcgccgag ccggatacag gctccgacat tctgcgggat gtctggttga
     2821 aaaagggcga gacgcattct aaggccgtca tacgcgtggc ctgggggctt gtcgcggtct
     2881 gatttgcgac tgtttcgcaa gaagcaacag caacttgtgg cgcatgccgc gagatgagac
     2941 cgggtcagcc tgcacggcct tgcactcgac atatcgccga cgcgacggcc ccgctcccgg
     3001 gggtcagagg cgccggccaa taagaggtga gacgtggccg aaccggtcgt tacagatggt
     3061 gtcgaaacgg acgccacccg ccgtgacttc attcacatcg cggccggggc catggccgtc
     3121 ggtgcgggtg cgatggtggc ctggcctctg atcaatcaga tgaaccccgc cgccgacacc
     3181 ttggccctgt cgaccatcga gttcgatgtg tccaaggttc aggaaggcca gcaggtcgtc
     3241 attacctggc agggcaagcc cgtgttcgtg cgctatcgga ccgccaagga aatccaggaa
     3301 gccaatgcgg cttctcccgg tgacctcaag gatccggaaa cggacgccgc ccgggtcaag
     3361 ccgggccatt cgcaatatct ggtcgtcatc ggctcctgca cccatctggg ctgcatcccg
     3421 acgttcggct cgggcgagtt cggcggctgg ttctgccctt gccacggttc ggtctacgac
     3481 acctcgggcc gcatccgtaa gggtcctgct ccgacgaacc tcgtggttcc ggaatacgcc
     3541 tacctgtcag acacgcgcat caagatcggt tgaggccaga ccagaccatg agcgatcacg
     3601 aatccacata cgttccccag accggcgtcg agaagtggct cgatacccgt ctgcccatca
     3661 tccgtttcgg cgctgactac ctgtcgctgc cgacgccgaa gaacctgaac tactggtaca
     3721 cgttcggcgg cattctggcg gtctgtctgg tcatccagat catcaccggc atcgtcctgg
     3781 cgatgcacta cacccccaac accgcgatgg cgttcgcctc ggtcgagcgc atcatgcgcg
     3841 acgtcaacgg cggctggttg atccgattca tccacgccaa cggcgcctcg atgttcttca
     3901 tcgccgtata catccacatg ctgcgcggcc tgtactacgg ctcgtataaa gctccgcgcg
     3961 agatgatctg gatcctcggc tgtgtgatct tcttcctgat gatcgccacc gcattcctcg
     4021 gctacgtcct gccgtggggc cagatgtcct actggggcgc agaggtcatc accaatctga
     4081 tcggggccat cccgctgatc ggtgagccga tcctgatctg gctgcgtggc ggacccggca
     4141 tcgacaacgc gacgctcaac cgcttcttct cgctgcacta cctgctgccg ttcgtgattt
     4201 tcggcgtggt cgtgctgcac ctctgggcgc tgcacacgtc gggccagaac aacccggtcg
     4261 gcatcctgat ccccaaggat cgcgagaaga aggacatggt ccccttccac ccgtattaca
     4321 cggtcaagga tggcttcgcc ctgctgctgt tcctgatcct gttctcggtg ttcgtgttct
     4381 tcatgccgga cagcctcggc cacgccgaca actacatcca ggcgaacccg ctgcagactc
     4441 cggcgcacat cgtgcccgaa tggtacatgc tgccgttcta cgcgatcctt cgcgcagttc
     4501 ctgacaagtt tggcggcgtc gtcgccatgt tcggctcgat cggcgtcctg ttcatcctgc
     4561 cttggctgga tacgtccaag gtgcgctcga tgcgctaccg cccgacgatg cggaccttct
     4621 tcctcatctt cgttttcgtc ggcatcggcc tcggctggtg cggtggtcag ctcccggacg
     4681 ctcacgtcat tccgggcctg agcaccttcg tcttcatcga cggcgacctg aactcctacc
     4741 tgtggctgac gcgtctgttc accgcctact acttcgcctt cttcttcatc atcctgccgc
     4801 tggtaggcct gaaggagacg ccgttgccga ttcccgcctc gatctcggag ccggtcctga
     4861 cgggctcgca tgcgatgtcc ggcgcggtcc ccgcccaagc cgaaaagaag ggctgagcgc
     4921 gatgacgttt ttcaagagca agactttggc ccttcgcaat accctcgcag ccatcacggc
     4981 cgtcgccggc ctcgcggtga tggcggcccc ggcctcggct gaaggcggtc accatctgcg
     5041 tgacggcggc ttcaccttcg aaggtccgtt cggcaccttc gatcagggcc agctgcagcg
     5101 cggctacaag gtctatcggg aggtctgcgc atcctgccac tcgatggacc tgatgcattt
     5161 ccggaccctc ggccagaagg gcgggccgtt ctacgacgag cacgccgcca acccggccga
     5221 gaaccgtttc gtcaaggcgc tggccgccga ggtgcaggtc gcggatatcg acaatgagac
     5281 cggcgaggcg atccagcgtc ccggcatcgc ggccgacacc ttcccgtcgc cctatccgaa
     5341 caagacagcc gcggccgccg caaacggtgg ggctgctccg ccggatctgt cggtgatggc
     5401 caaggcccgt cacggcggtg cgaactacat ctattcg
//
LOCUS       DASVTM010000086        10771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_965351,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000086 DASVTM010000000
VERSION     DASVTM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965351"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..3468)
                     /gene="mfd"
                     /locus_tag="VF633_02345"
     CDS             complement(34..3468)
                     /gene="mfd"
                     /locus_tag="VF633_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX8469928.1"
                     /translation="MSRTDIELGGAPEGLDALTVAERLTAAGGVGVFVARDYQRAGNF
                     AQAFRFFAKDIDVLEFPAWDCLPYDRLSPTSSVSAERMATLTELAGRPARAARPLLVV
                     TTIAAAMQRTPPQEVTVSAGFDAAVGRDLDQPALEKYFVTNGYMRASTVNERGEYAVR
                     GGVIDVFPPGFEEPVRLDMFGSELESIRTFDPATQRSTGQKKKISLSPVSEALLDPET
                     ISRFRTGYLGLFGAGGDDPLYVAVSEGARRQGMEHWLPLLYPQLETLFDYLPDDAAVF
                     LDNQADAARHERWALASDAYEARREASLTKGGAANRALPPDRLYIPDQDWNSALAGRA
                     VRRLTPFDTTGEDAGGRLGKTFAAERAQDSVNLFEAVAKHAEALKGEGKRVLFASWSE
                     GSSERLSIMLADHGLDHVVAVRDWADVQSAPKDLYLRAVLPVEHGFTTDNLAVISETD
                     MLGDRLARPKRKRRASNFLAEASALTTGDLVVHLDHGIGRYEGLKTLEIQEAPHDCLE
                     LLYAGESKLYLPVENIDLLTRYGTDSEDVQLDRLGGAAWQGRKAKAKSRLREMAEGLI
                     ALAAKRSLRVSDAITPPHGLFDEFCARFPYEETDDQLNAIGDVLEDLGKGTPMDRLIC
                     GDVGFGKTEVALRAAFVVAMTGQQVAVVCPTTLLARQHFKTFSERFAGWPITVRHLSR
                     MVTAKDAAESRAGLKDGKFEIVVGTHAVLSEQVGFKDLGLVIVDEEQHFGVKHKEKLK
                     SLRADVHLLTLTATPIPRTLQMALSGIREMSIIATPPVDRLAVRTYVTPWDRVLIREA
                     LLREKYRGGQAFYVCPRLSDLPEVEHFLREQVPEIKFVVGHGQMSPTQLEDVMSAFYD
                     GEYDLLVSTTIVESGIDIPTANTLIVHRADMFGLAQLHQIRGRIGRSKARAFAYLTTD
                     PKRPLTLSAERRLQVLQSLDNLGAGFQLASHDLDQRGGGNLLGDEQTGHMREVGVELY
                     QQMLEDAVASLRDAGEGDTVDRGWSPAINVGAAVLIPEAYVPDLNVRLSLYRRLSDAE
                     KTEEREALAAELIDRFGPLPDEAKQLLRIVGIKANCKTACIERIDIGPKGCVLTLRNN
                     TFPNPMGLVGLIQKNHAFWKIRPDQKIVVKGDWPTPAERLKVAERITTDLARVAGA"
     gene            complement(3481..3786)
                     /locus_tag="VF633_02350"
     CDS             complement(3481..3786)
                     /locus_tag="VF633_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX8469929.1"
                     /translation="MTDTNARLKERLDRIRFRAWRRGFREADMVLGPFADRVGPELTD
                     AELTEFESLLAEDNDHVLYSWIIETAPTPPEHDTLLLGKLRAFVKAHVAHAVQQGIG"
     gene            3932..6022
                     /gene="recG"
                     /locus_tag="VF633_02355"
     CDS             3932..6022
                     /gene="recG"
                     /locus_tag="VF633_02355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX8469930.1"
                     /translation="MRPQILFPLFAEVTSLKGVGAKVAPLVQKLAGPLVRDVLFLSPN
                     GLIVRRRTTAAEAVDGEVGVFDVVIDRLIVPGKPGAPLKVRASDDTGYIHMVWFGGSP
                     QHIDRQLPRGERRLVSGKVERFNGEVQILHPDWIVALDKAEEIPTVEPVYPATAGLTS
                     RVVRKLAQGALATAPELPEWQDAAWMAARRWPGWRPALEALHSPQTEFDLGPDAPVRQ
                     RLAYDELFAHQLALARRRRARQITPAPKIAPGEASAALLTALPFALTGAQAQALAEIR
                     VDLASGEQMGRLLQGDVGSGKTAVAALAMADAASSGFQAALMAPTEILVRQHWQRLSP
                     MLESAGVTSVLLTGRDTTVERRERLDALATGRAQVAIGTHALFQDAVRFDRLGLAVID
                     EQHRFGVNERQRLQAKGDPRVGGVHLLTMSATPIPRTLELTQYGELEVSRLMEKPPGR
                     TPIATAVLPTARIGEVAKRLKAAIEGGAQAYWICPLVAESEASDLAAAEARAEDLKRI
                     LQIEVGLAHGQMPGAQREAVMAGFADGRIPLLVATTVVEVGVDVPNASIMVIEHADRF
                     GLAQLHQLRGRVGRGAKASACILLYGSGENGLGDTAKERLETLRRTEDGFEIAEEDFR
                     LRGGGDPLGLKQSGFPAYRFADPVRHRSLMLAAADDARLVLGRDPDLLSERGQAVRTL
                     EALFDWRNDRSGLD"
     gene            complement(6025..7134)
                     /locus_tag="VF633_02360"
     CDS             complement(6025..7134)
                     /locus_tag="VF633_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX8469931.1"
                     /translation="MTRARLTFRQWVGWFGFAFVLLIVAATAFWRGDILRAGLDPQVP
                     FQTYEPPPAPDYAKPSSWALLDARTPSAGDAQIFFVHSTTFDGGPDWNGPIDDAEADA
                     YLRRVVIPNYAGPFEQAGAISIPRYRQASLYTRLTLRMDARDARAFAYDDILTAFDIW
                     LERHPSGPIVLSGVEQGADLLDRLVRERIASDPAVRRRLVAAYLVDTIIAADNLSPDV
                     PACASRQQTGCVVAWSQVGEGDDGAAERRLRRALVWNARGRLVELAGRQPLCVNPVSG
                     MTGTEAVPARRHLGAANATGLEWGVRPAFLDRLIATQCRGGLLRHTMPDTESFREVGD
                     WADRRKARQYNLFYADTQADVQDRLTAWKAANPAG"
     gene            complement(7131..8132)
                     /gene="rfaD"
                     /locus_tag="VF633_02365"
     CDS             complement(7131..8132)
                     /gene="rfaD"
                     /locus_tag="VF633_02365"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX8469932.1"
                     /translation="MSRKVVVVTGGAGFIGSNIVARLTAEDRYDVVVCDRLETAELAK
                     WKNLAKHPIADFWQPEELFEMLERQAEEIEAVVHMGAISATTEPDADLILRTNFTLSR
                     DLWDWCSIRDARFIYASSAATYGDGDQGFEDDDSLPALASLRPLNAYGYSKMLFDQYA
                     VRQSDRGQSPSQWAGLKFFNVYGPNEGHKGSMKSVVAQIWPQVAAGEPVILFRSHNPD
                     YADGGQMRDFVYVDDVVDIVVWLLDNPHAFGVFNAGSGQARSFLDLANATFAAAGKTP
                     EIAYVDTPVSIRDRYQYFTEAKMDRIRAAGYAGQSTPLEEGVRRYVQSYLSQPDQYR"
     gene            complement(8129..9601)
                     /gene="rfaE2"
                     /locus_tag="VF633_02370"
     CDS             complement(8129..9601)
                     /gene="rfaE2"
                     /locus_tag="VF633_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX8469933.1"
                     /translation="MAENSSDRTLDLGALQILLDRVAGLKIACVGDLMLDRYVYGEVT
                     RVSPEAPIPVLKTKRTVSMPGGVGNVARNVAALGGEAHLGAVAGPDAAGDELVALIAA
                     DDRVSDFVDRPAGASTIVKTRFVSAGQQLLRLDEEELAVGVFDREDVFSDTSAILLSD
                     YDKGVVTERLIRSALWAGQNAGVPVIVDPKGRDFARYGAVDVIKPNASELAGATGLPV
                     GTDAEVEAALAHLLSITTAKAIVVTRAGKGMSLVRRDGPIQHFPGRAREVFDVSGAGD
                     TGLAALGLALGAGATLEQAVELAILASGVVVGKSGTAVVTPAELIEAEMSQHASLAYA
                     KVTPVDELAAQVEAWRRQGLRVGFTNGCFDILHRGHVAYLAQARSWCDRLVVALNTDA
                     SVRRLKGEGRPVNDLDSRAVVIGGLASVDRVTAFDDPTPIALIERLRPDVLIKGADYT
                     REGVVGGDLVESWGGEVRLAAFQDGYSTTKTIEKLSGDAA"
     gene            9744..>10771
                     /locus_tag="VF633_02375"
     CDS             9744..>10771
                     /locus_tag="VF633_02375"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX8469934.1"
                     /translation="MPTEKPVVLWFRRDLRLADNPALSHAVETGRPVIPLYILDGAGH
                     GRALGAASLWWLDKSLRALGDSLKATGAPLILRRGDSEAELRRLIDETGADVVLMNRL
                     FEPDAWVRDADIAHALQDDGVECRGFNGSLMTKPGSVLNGTGGPYKVFTPFKNALLAR
                     AEPPPPTAGPRALETISGLASDNIDDWGLHPTNPDWSHGFDWTPGEAAAVGALTLFTG
                     SALKSYSRGRDHPAEVGGSRLSPHLHWGEVHPWRVLEAVQQAAAEGRVPQAEADKFVA
                     EIAWREFSAHLLHAFPQITDRSFRPEYDQFPWLNDDAGFAAWTRGQTGYPIVDAGMRQ
                     MWTTGWMH"
BASE COUNT         1815 a         3402 c         3797 g         1757 t
ORIGIN      
        1 gggcgaaggg gcttttcaag cagtaatggc tttctacgcc cccgccaccc tcgccagatc
       61 ggtggtgatg cgttcggcga ccttgaggcg ttcggcgggg gtgggccagt cgcccttgac
      121 gacgatcttc tgatcgggac ggatcttcca gaaggcgtgg ttcttctgga tcaggccgac
      181 cagacccatc gggttcggga aggtgttgtt gcgcagggta aggacacagc ccttcggtcc
      241 gatatcgatg cgttcgatac aggcggtctt gcagttggcc ttgatgccga cgatgcgcag
      301 caactgcttc gcctcatccg gcagcgggcc gaaccggtcg atgagttcgg cggccagggc
      361 ttcgcgttct tcggtcttct cggcgtcgga cagacggcgg tagaggctga gacggacgtt
      421 caggtccggg acgtaagcct ctggaatcag gaccgctgca ccgacgttga tggcgggaga
      481 ccagccgcga tcgacggtgt ccccctcgcc tgcgtcgcgc agggaggcta cggcgtcttc
      541 gagcatctgc tgataaagtt cgacgccgac ctcgcgcatg tgacccgtct gttcgtcgcc
      601 cagcaggttg ccgccgccgc gctggtcgag gtcgtggctg gcgagctgaa agcctgcgcc
      661 gaggttatcc agcgattgta gcacctgcag cctacgctct gccgacaagg tcagcggacg
      721 cttgggatcg gtggtcagat aggcgaaggc gcgggccttg gaacggccga tacggccccg
      781 gatctggtgc aactgcgcca ggccgaacat gtctgcgcgg tggacaatca gggtgttggc
      841 ggtcgggatg tcgatgccgc tctcgacgat cgtggtcgag accagcaagt cgtactcgcc
      901 gtcatagaag gcgctcatga cgtcttcgag ctgggtcgga gacatctggc cgtgaccgac
      961 aacgaacttg atctcgggga cctgttcgcg aaggaaatgc tcgacctcgg gcaggtctga
     1021 cagacgtggg cagacgtaga aggcctgacc gccacgatac ttctcgcgca gcaaggcctc
     1081 gcggatcagc acccggtccc acggcgtcac ataggtccgc acggccagac gatcgacggg
     1141 cggggtggcg atgatcgaca tctcccggat gcccgacaga gccatctgca gggtgcgcgg
     1201 gatcggtgtc gcggtcaggg tcagaaggtg gacgtcggca cggaggctct tgagcttctc
     1261 cttgtgcttg accccgaaat gctgctcttc atcaacaata acaaggccca aatccttgaa
     1321 cccaacctgt tccgagagga cggcgtgggt gccgaccacg atctcgaact tgccgtcctt
     1381 gaggcctgcg cggctttcgg cggcgtcctt ggcggtgacc atgcgcgaca ggtggcggac
     1441 cgtgatcggc cagccggcga agcgttcgga gaaggtcttg aaatgctggc gggccaacaa
     1501 ggttgtcggg cagacgacgg cgacctgctg gccagtcatg gccacgacga aggcggcgcg
     1561 cagggcgacc tcggtcttgc cgaagccgac atcgccgcag atcagccggt ccatcggcgt
     1621 gcccttgccg aggtcctcaa gcacatcgcc gatggcgttg agctgatcgt ccgtctcctc
     1681 ataggggaag cgggcgcaga actcgtcgaa caggccgtgc ggcggagtga ttgcgtcgga
     1741 cacgcgcagc gaacgctttg cggcgagggc gatgagaccc tcggccattt cgcgcagtcg
     1801 ggacttggcc ttggccttac gcccctgcca ggcggcgccg cccagacgat cgagttgaac
     1861 gtcttcggag tccgtgccgt agcgggtcag aaggtcaatg ttctcaaccg gaaggtagag
     1921 cttgctctca ccggcataca gaagttcgag gcagtcgtgc ggagcttcct ggatctccag
     1981 cgtcttcagc ccctcgtagc ggccgatgcc gtggtcgagg tggacgacga gatcgccagt
     2041 cgtcagggcg gaggcctcgg cgaggaagtt ggaggcgcga cgcttgcgct tgggccgggc
     2101 cagccggtcg ccgagcatgt cggtctcgga gatgaccgca aggttgtcgg tggtgaagcc
     2161 atgctcgacc ggcaggaccg cacgcagata caggtccttt ggcgccgatt ggacatcggc
     2221 ccagtcgcgc acggcgacga cgtgatccag cccgtggtcg gccagcatga tcgacagacg
     2281 ctcggacgaa ccctcggacc acgaggcgaa caggacacgc ttgccctcgc ctttcagcgc
     2341 ctcggcgtgt ttggcgacgg cctcgaacag attgacgctg tcctgcgcac gctcggcggc
     2401 gaaggtcttg cccagtcgac cgccggcgtc ttcgcctgtg gtgtcgaacg gcgtcaggcg
     2461 acgaaccgcg cgccctgcga gtgcgctgtt ccagtcctga tcggggatat agaggcggtc
     2521 gggcgggagg gcgcgattgg ccgcgccgcc cttggtcagc gaggcctcgc gacgggcctc
     2581 gtaggcgtcg gaagccagag cccagcgctc gtgccgggcg gcatcggcct gattgtcgag
     2641 gaacaccgcc gcgtcgtcgg gcagatagtc gaacagggtc tcaagctgcg gatagagcag
     2701 cggcagccag tgctccatgc cctgacggcg tgcaccctcg gacacggcga catagagggg
     2761 atcgtccccg cccgcgccga acaggccgag atagccggtg cggaagcgcg atatggtctc
     2821 ggggtccagc agggcctcgg acaccgggga cagggagatc ttcttcttct ggccggtcga
     2881 gcgttgggtc gccggatcga aggtgcggat cgattccagc tcggagccga acatgtcgag
     2941 gcgcacaggc tcctcgaagc ccggcggaaa gacgtcgata accccgccgc gcaccgcgta
     3001 ttcgccgcgc tcgttgacgg tcgaggcgcg catatagccg ttggtgacaa agtatttctc
     3061 gagcgcaggc tggtcgagat cgcggccgac ggcggcgtca aagccggccg agaccgtgac
     3121 ctcctgcggg ggcgtgcgct gcatggccgc ggcgatggtc gtcacgacca gcaggggtct
     3181 ggcagcgcgc gcggggcgac cggccagttc ggtcagtgtc gccatccgct cagcggagac
     3241 cgacgaggtc gggctcaacc ggtcgtaggg caggcagtcc caggccggga actcgaggac
     3301 atcgatgtcc ttggcgaaga aacggaacgc ttgagcgaaa ttgccggcgc gctggtaatc
     3361 gcgcgccacg aacacgccga cgccgcctgc agcggtcagc cgctcggcga cggtcagggc
     3421 gtcgagaccc tcgggcgcgc cgcccagttc gatgtcggtg cggctcaatt taacgctcac
     3481 tcagccgatc ccctgctgca cggcatgggc gacgtgggct ttaacgaaag cgcgcagctt
     3541 gcccagcagc agcgtgtcgt gctcgggcgg cgtcggcgcc gtctcgatga tccagctgta
     3601 gaggacgtga tcgttgtcct cagccaacag gctttcgaac tcggtcagct cggcgtcggt
     3661 cagttcgggc ccgacacgat cggcgaacgg gcccagcacc atgtcggcct cgcgaaaacc
     3721 gcgccgccaa gcgcggaagc ggatccggtc cagtcgttct ttcagacgcg cgttcgtatc
     3781 ggtcacaagg atccttgccc ggtcggttca gtgaaatgtg aggtcgcaga tagtccgtcg
     3841 ccgggcggtt tgccagtaaa tcagcgcaga gaacccgcgc tgccgccaat cgcatggcgc
     3901 gcccgggtac tgccccgcta tgctgcgacg aatgcggccc cagattctct ttcccctgtt
     3961 tgcagaggtc accagcctga agggcgtggg ggccaaggtc gcgcctctgg tccagaaact
     4021 ggccgggccg ctggtgcgcg acgtgctgtt cctgagtccc aacggcctga tcgtgcgccg
     4081 ccggacgacc gccgctgagg cggtggatgg cgaggtcggg gtgttcgacg tggtcatcga
     4141 ccggctgatc gttccgggta agcccggcgc gccgctcaag gtccgcgcct ccgacgacac
     4201 cggctatatc cacatggtct ggttcggcgg ctcgccgcag cacatcgacc ggcagcttcc
     4261 acggggcgaa cggcggctgg tgtcaggcaa ggtcgagcgg ttcaacggcg aggtgcagat
     4321 cctgcatccg gactggatcg tggcgctgga caaggccgag gagattccga cggtcgagcc
     4381 cgtctatccc gccaccgctg ggctgacctc gcgcgtggtt cggaagctcg cgcagggcgc
     4441 gctggctacg gctcccgaac tgccggagtg gcaggacgcg gcgtggatgg cggcgaggcg
     4501 ctggcccggg tggcgaccgg cgctggaggc gctgcattca ccgcagaccg agttcgatct
     4561 cgggccggac gcgccggtgc ggcagcggct ggcctatgac gagttgttcg cacaccaatt
     4621 ggcgctggcc cgccggcgtc gggcgcgcca gatcacgccc gcgccgaaga tcgcgccggg
     4681 tgaggcttcg gcggcgctgc tgaccgccct gcccttcgcc ttgacgggcg cgcaggcgca
     4741 ggcgctggcg gaaatccgcg ttgatctggc gtcaggcgaa cagatgggcc ggttgctgca
     4801 gggcgacgtg ggctcgggca agacggcggt cgcggcgctg gccatggccg acgcggcgtc
     4861 aagcggcttt caggcggctc tgatggcccc cacagagatc ctcgtccgcc agcactggca
     4921 gaggctgtcg ccgatgctgg agagcgcggg cgtgacgtcg gtcctgctga ccgggcgcga
     4981 cacgacggtc gaacgacgcg aacggctcga cgcactcgcg accggtcgag cccaggtcgc
     5041 catcggcacc catgccctgt ttcaggacgc cgtgcgcttt gaccgtctgg ggttggccgt
     5101 gatcgacgaa cagcaccggt tcggcgtcaa cgagcgccag cggctgcagg ccaagggtga
     5161 tccgcgcgtg ggcggggtgc atctgctgac catgtccgca acgccgatcc cgcgcacgct
     5221 ggagctgacg caatacggcg agttggaggt cagccggctg atggagaagc cgcccgggcg
     5281 cacgccgatc gctaccgccg tcctgccgac cgcacgcatc ggcgaggtgg ccaagcggct
     5341 gaaggcggcg atcgagggcg gggcccaggc ctactggatt tgtcctctgg tggccgagag
     5401 cgaggccagc gatctggcgg cggccgaggc gcgcgccgag gacctgaaac gcatcctgca
     5461 gatcgaggtg ggtctggcgc acggccagat gccgggcgcg cagcgcgagg cggtgatggc
     5521 cggcttcgcc gacggacgta tcccgctgct ggttgcgacg acggtggtcg aggtgggggt
     5581 cgatgtgccc aacgcctcga tcatggtcat cgaacatgcc gaccggtttg gattggcgca
     5641 gctgcaccag ttgcgcggac gggttgggcg tggcgccaag gccagcgcct gtatcttgct
     5701 ctacggtagt ggcgagaacg gcttgggcga caccgccaag gaacggctgg agaccctgcg
     5761 tcgtaccgag gacggattcg agatcgccga ggaggacttc cgcctgcgcg ggggcggcga
     5821 tccgctgggg ctcaagcaga gcggttttcc cgcctaccgg ttcgccgatc ccgtgcggca
     5881 tcgcagcctg atgctggcgg ccgccgacga cgcgcggctg gtgctcgggc gcgatccaga
     5941 tctgcttagc gagaggggcc aggcggtgcg gacgctggag gcgctgttcg actggcgcaa
     6001 cgatcggtcg gggctggact aaccctagcc tgccgggttg gcggccttcc aggcggtcag
     6061 acgatcctga acgtcggcct gagtatcggc gtagaacagg ttgtattgac gcgcttttcg
     6121 tcgatcggcc cagtcgccga cttcgcggaa ggactccgtg tccggcatag tgtggcgcag
     6181 gagcccgcca cggcactggg tggcgatgag acggtcaagg aaggccgggc ggacgcccca
     6241 ttcgagcccg gtggcgttgg cggcgcccag atgacgacgg gcggggacgg cctcagttcc
     6301 ggtcatgccg ctgactggat tgacgcacag cggttgacgg ccagcgagtt cgaccagccg
     6361 gccacgggcg ttccagacga gggctcgccg caaccgccgc tccgccgccc cgtcgtcacc
     6421 ctcaccgacc tgcgaccagg caacgacaca gcccgtttgc tgtcgggaag cacaggcggg
     6481 aacatcgggg ctgaggttgt ccgccgcgat gatggtgtcg accagatagg cggccaccag
     6541 tctgcgtcgg acggcggggt cggaggcgat ccgctcgcgc accaaccggt ccagcagatc
     6601 ggcgccctgc tcgacgccgg aaagcacgat cggccccgac ggatggcgct caagccagat
     6661 gtcgaaagcg gtcaggatat cgtcataggc gaaggctcgg gcgtcgcggg cgtccatccg
     6721 caaggtcagc cgggtgtaga gactggcctg ccggtagcgc ggaatgctga tggcccccgc
     6781 ctgctcgaac ggtccggcgt agttggggat gaccacgcgg cgcagatagg cgtcggcctc
     6841 ggcgtcgtcg atgggaccat tccagtcggg accgccatcg aaggtggtgg aatggacgaa
     6901 gaatatctgc gcgtcacctg cggaaggtgt tcgtgcgtcg agaagcgccc agctggacgg
     6961 cttggcgtaa tcgggcgcgg gcggcggttc ataggtctgg aacgggacct gcgggtcgag
     7021 accggccctg agaatatcgc cgcgccagaa ggccgtcgcc gcgacgatca gcaagacgaa
     7081 cgcaaagccg aaccagccga cccactggcg aaaggtcagc cgcgcacggg tcaccgatat
     7141 tgatccggtt gggagagata cgactggacg tagcgacgca cgccctcttc gagcggcgtc
     7201 gactggcccg cgtatccggc ggcgcggatg cgatccatct tcgcctcggt gaaatactgg
     7261 tagcggtcgc ggatcgagac cggggtgtcg acgtaggcga tctcaggcgt cttgcccgcc
     7321 gctgcgaagg tggcattggc gaggtcgagg aaggaccgcg cctgacccga accggcattg
     7381 aagacgccaa aggcgtgcgg gttgtccagc agccacacga cgatatcgac cacgtcgtcg
     7441 acatagacga agtcgcgcat ctggccgccg tcggcgtagt ccgggttgtg cgagcggaac
     7501 aggatgacgg gctcgcctgc cgcgacctgg ggccagatct gggccacgac cgacttcatc
     7561 gaccccttgt gaccctcgtt gggaccgtag acgttgaaga acttcagccc ggcccactgg
     7621 ctcggagact gaccgcgatc cgactgacgc acggcgtact ggtcgaacaa cattttcgaa
     7681 tagccgtagg cgtttagcgg gcgaagcgat gccagggccg gaagactgtc gtcatcctcg
     7741 aagccctgat cgccatcgcc gtaggtggcc gccgaagagg cgtagatgaa gcgggcgtcg
     7801 cggatcgaac accagtccca cagatcgcgg gacagggtga agttggtgcg caggatcagg
     7861 tcggcgtcgg gctcggtcgt cgccgaaatc gcgcccatgt ggacgaccgc ctcgatctcc
     7921 tcggcctggc gttcaagcat ttcgaacagc tcttccggtt gccaaaagtc ggcgatcggg
     7981 tgtttggcga ggttcttcca cttggccagt tcggcggtct caagccgatc gcaaacgacc
     8041 acatcatagc gatcctcagc cgtcagccgc gcgacgatgt tggagccgat gaagcccgcc
     8101 ccgccggtga ccacgacgac cttccggctc atgccgcatc tcccgagagc ttctcaatgg
     8161 ttttcgttgt cgagtagccg tcctggaacg cggccagccg gacctccccg ccccagctct
     8221 cgaccagatc gccgccgacc acgccttcgc gcgtatagtc agcgcccttg atcagaacat
     8281 cgggacgcag gcgttcgatc agggcgatgg gggtcgggtc atcgaaggcg gtgacccggt
     8341 ccacgctggc gaggccgccg atgacgacgg cgcggctgtc gagatcgttg accggtcggc
     8401 cctcgccttt caaccgtctg accgaggcgt cggtgttcag ggcgaccacc agccggtcgc
     8461 accaactgcg cgcctgcgcc agataggcga cgtggccgcg gtgcaggatg tcgaagcagc
     8521 cattggtgaa accgaccctc agtccctgac ggcgccaggc ctcgacctga gccgcgagtt
     8581 catcgacggg cgtgaccttg gcgtaggcca gcgaggcatg ctggctcatc tcggcctcga
     8641 tcagttcggc gggcgtgacg acggcggtgc cgctctttcc aaccactacg cccgaggcga
     8701 ggatggccag ctcgacggcc tgctccagcg tcgcgcccgc gccgagcgcc agaccgagag
     8761 ccgcaaggcc ggtatcgcct gcgccggaga cgtcgaacac ctcgcgtgcg cgaccgggga
     8821 aatgctggat ggggccatca cggcgtacga gcgacatgcc tttacccgca cgggtgacga
     8881 cgatggcctt ggccgtggtg atggacaaca ggtgcgcgag ggcggcctcg acctcggcgt
     8941 ccgtgccgac cggcaggccg gtggcgcccg ccagttcgga ggcgttgggc ttgatgacat
     9001 cgaccgcgcc gtatcgggcg aagtcacgcc ctttgggatc gacgatgacg ggaacgccag
     9061 cattctgacc ggcccagagc gccgagcgga tcaggcgttc ggtgacgacg cccttgtcgt
     9121 agtccgacag aaggatggcg gaagtgtctg aaaacacgtc ctcgcggtcg aacacgccga
     9181 cggcgagttc ctcttcgtcc aagcgcaaaa gctgctggcc ggccgagacg aagcgggtct
     9241 tgacgatggt gctcgcgccc gccggacggt cgacgaaatc agagacgcgg tcgtcggcag
     9301 cgatcagggc gaccaactca tcgcccgctg catcgggtcc ggcgacggcg cccagatggg
     9361 cctctccacc tagagccgcg acgttgcggg cgacattgcc gacgccgccg ggcatcgaca
     9421 cggtgcgctt ggtcttgagc acggggatcg gagcctcggg cgagacgcgg gtgacctcgc
     9481 catagacgta gcggtccagc atcaggtcgc cgacgcaggc gatcttcaga cccgcgacgc
     9541 ggtccagcag gatctgcagg gcgccgagat cgagcgtccg atcagaagag ttttcagcca
     9601 tgccgtcgaa ctaccggatt cagcccgctt tcgccatcgg cggtttcgcg agtggggaaa
     9661 ccttcgcagt cgccgaggct tctgtcggac aggctgcgcg acgacgacca tcgcactcgc
     9721 agaccaattc cggagacgca cgcttgccca ccgaaaaacc cgtcgtcctg tggttccgtc
     9781 gcgatctgcg gctggcggac aatccagccc tgtctcatgc ggttgagacg ggtcggccgg
     9841 tgatcccact ctatatcctg gatggcgccg ggcatggtcg agcgctcggc gcggcgtcgc
     9901 tgtggtggct cgacaagtcg ctgcgggcct tgggtgacag cctgaaggca acaggcgcgc
     9961 cgctgatcct gaggcggggc gattctgaag ctgaactgcg tcgcctgatc gacgagaccg
    10021 gcgccgacgt cgtgttgatg aaccgactgt tcgagcccga cgcctgggtc cgcgacgccg
    10081 atatcgccca tgcgctgcag gacgacggcg tcgaatgccg gggtttcaac ggctcgctga
    10141 tgacaaagcc gggatcggtt ctgaacggga ccggcgggcc gtacaaggtt ttcacgccgt
    10201 tcaagaacgc gctgctggcg agggccgagc cgcctccacc cacggcaggg ccgcgcgcac
    10261 tggagacgat ctcggggttg gccagcgaca acatcgatga ctgggggctg cacccgacca
    10321 accccgattg gtcccacggc tttgactgga cgccgggcga ggccgccgcc gttggggcgt
    10381 tgacgctgtt caccggttcg gcgttgaagt cctattcgag ggggcgcgac cacccggcag
    10441 aggttggggg aagccggttg tcccctcacc tgcactgggg cgaggtccac ccgtggcggg
    10501 tcctcgaggc cgtgcagcag gccgccgcag agggtcgcgt accacaggcc gaggcggaca
    10561 agttcgtggc cgaaatcgca tggcgcgagt tctcggcgca cctgctccac gctttcccgc
    10621 agatcaccga ccggtcgttc cggcccgagt acgaccagtt cccgtggttg aacgacgacg
    10681 ccggctttgc ggcgtggacc cgcggccaaa cgggctatcc gatcgtagac gccggcatgc
    10741 gccagatgtg gactacgggc tggatgcaca a
//
LOCUS       DASVTM010000087        13170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_488093,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000087 DASVTM010000000
VERSION     DASVTM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 13170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13170
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488093"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VF633_02380"
     CDS             complement(<1..774)
                     /locus_tag="VF633_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8469935.1"
                     /translation="MASSTNKKVIFAALAGNLAIAATKLIAAVFTGSAAMMSEAIHSA
                     VDTGNQVILLIGLKRAARPPTENHPFGHGLQLYFYTFVVAVLIFGIGAVISITHGIER
                     IQNPEPIENPWVNYLILGLSMVFEGASWTIAFRAFNKERGGRPFLGMVHTSKDPTVFT
                     VLFEDTAALAGLVVALVGVVCSQVFDMPVLDGVASVAIGLILAVTAFFLASESQSLLT
                     GEAADPATREGIKAIATAEPGVEGLNDARTMHFGPNEIMV"
     gene            1036..1590
                     /locus_tag="VF633_02385"
     CDS             1036..1590
                     /locus_tag="VF633_02385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX8469936.1"
                     /translation="MADDFRTTEVGERYARALFDLALETGKLDAVRADLKSLKAAWLE
                     SADLRRLATSAIISADDQAKGLVAIAAKAKFETTTRNFLGLLAQNGRARDLAAVTTGF
                     EALYAKHAGVVAAEVVSTQELTVVQLTHIRNALRQALGKAPEITTRIDPSILGGLKVK
                     VGSKLFDASLKTKLDQMKFALRRA"
     gene            1678..3213
                     /gene="atpA"
                     /locus_tag="VF633_02390"
     CDS             1678..3213
                     /gene="atpA"
                     /locus_tag="VF633_02390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX8469937.1"
                     /translation="MDIRAAEISAILKSQIANFGVEADVSDVGSVLSVGDGIARIHGL
                     DNVQAGEMIEFPKAGVKGMALNLERDNVGAVIFGADAQIAEGDEVRRLGEIVDVPVGK
                     GLLGRVVNPLGEPIDGKGPIQFTERRRVDVKAPGIIPRKSVHEPMQTGLKAIDTLIPV
                     GRGQRELIIGDRQVGKTAVAIDTILNQKVTNAAATSEGDKLYCIYVAIGQKRSTVAQI
                     VKTLEERGALEYTIVVAATASEPAPLQFLAPFSGTAMGEFFRDNGMHALIVYDDLSKQ
                     AVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERSAKLNEDNGSGSMTALPLIETQAN
                     DVSAYIPTNVISITDGQIFLESDLFYQGIRPAVNVGISVSRVGSSAQIKAMKQVAGSI
                     KGELAQYREMAAFAKFGSDLDVSTQKLLARGARLTELLKQPQYSPLTVEEQVVSIYAG
                     TRGFLDAIAVSDIGRFESELLARMHSNHAGILDAIRTQKALSKDLEAELKAAIDAFAK
                     TFA"
     gene            3243..4121
                     /locus_tag="VF633_02395"
     CDS             3243..4121
                     /locus_tag="VF633_02395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX8469938.1"
                     /translation="MASLKEMRNRIESVKSTQKITKALNMVAAAKLKRAQESAENARP
                     YARKMAAVIANLAAGVSGDSAPKLLAGTGSDKKHLVVVATGDKGLAGGFNTNVIRAAR
                     DRINSLIANGKDVRIIAVGRKVRDGLTRLFGDKIIRTFELTGHKVIGMATAEPIAELI
                     ADEFESGNADVVTLIFSRFQSVISQVPTPRQLIPAVVDGAAEPINLNGAVYDYEPSEE
                     EILETLLPRNIKTQILAALLENQASFFGAQMGAMDNATRNAGELITALRLTYNRKRQA
                     QITTELIEIIAGAEAL"
     gene            4164..5696
                     /gene="atpD"
                     /locus_tag="VF633_02400"
     CDS             4164..5696
                     /gene="atpD"
                     /locus_tag="VF633_02400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX8469939.1"
                     /translation="MTDTVAKKPAAPRKPKAPAEVAATGNAAYTAGTGVGKIAQVIGA
                     VVDVEFVGELPAILNALETTNTDQKTGEPFRLVLEVAQHLGENMVRTIAMDTTEGLTR
                     GQPVTDTGTSILAPVGPGTLGRIMNVVGDPIDEGGPILTTMYRPIHREAPSFEEQSTS
                     SEILVTGIKVIDLICPYTKGGKTGLFGGAGVGKTVTMQELINNIAKAYGGYSVLAGVG
                     ERTREGNDLYHEMIESNVNVAGGGEGSKCALVYGQMNEPPGARARVALTGLAQAEYFR
                     DEEGKDVLLFIDNIFRFTQAGSEMSALLGRIPSAVGYQPTLATEMGNLQERITSTKKG
                     SITSIQAIYVPADDLTDPAPAASFAHLDATTVLNRDIAAQAIFPAVDPLDSTSRIMDP
                     LVIGEEHYRVARSVQEILQQYKALKDIIAILGMDELSEEDKLVVSRARKISRFLSQPF
                     FVAEQFTNAPGKFVALADTIRSFKGIVAGEYDHLPEAAFYMVGAIEEAVEKAQKMASE
                     AS"
     gene            5699..5968
                     /locus_tag="VF633_02405"
     CDS             5699..5968
                     /locus_tag="VF633_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX8469940.1"
                     /translation="MAGKLQFSLVAPEREVFTGLVDQVDAPGVEGDFGVLPEHAPFMT
                     ALREGVVTVFDGSTRRVFEVHGGFADVTPAGLTILAEQASEVSAT"
     gene            5990..6937
                     /locus_tag="VF633_02410"
     CDS             5990..6937
                     /locus_tag="VF633_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8469941.1"
                     /translation="MILTLAWIGAVVSPLVCALLIRRLLRSRRIIFAGALTAITAAAW
                     VLGVWAFLVEPATLTVRHVTVESAQWRGPPLRIGLISDTHVAAPHTDVRRVEHLVARM
                     NAQHPHVVMLLGDYAGGHEPAATRAHPEQSEILRGIEAFRSLTAPFGVHGVLGNHDSW
                     YDDAAISGALRRAGASVLDNRAERIERPGGAFWIAGLADIESPRDPPDVAATLAQVTD
                     DAPVIVLSHWPDPFAEVPGRVAITLAGHTHCGQVNLPLFGRVVHASRMSKRWACGLYD
                     EGGRKLFVTGGVGVSILPVRFRAPPEIVILTLKSAGAHS"
     gene            6934..7173
                     /locus_tag="VF633_02415"
     CDS             6934..7173
                     /locus_tag="VF633_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469942.1"
                     /translation="MSLIDIAGVFGVLLILIAYAGATAGKLDPKQWPALVLNLVGALL
                     ILWSLSVDFNLSAALMEGAWAIVAVLGLVRLVMKR"
     gene            complement(7208..7690)
                     /locus_tag="VF633_02420"
     CDS             complement(7208..7690)
                     /locus_tag="VF633_02420"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX8469943.1"
                     /translation="MQDLSLYRPNVGVVLFNASGQVWYGKRAGVGGPHNWQFPQGGVD
                     KGEDLEAAALRELQEETGVTSVELLGRTESEIIYDFPAELMAQHLREKRKPWNGQRQI
                     WFAYRFIGDEAEIDLNQHEEIEFDDWRWGDLSEACDLIVPFKRAAYEQVVAAFAKFVG
                     "
     gene            complement(7800..9011)
                     /locus_tag="VF633_02425"
     CDS             complement(7800..9011)
                     /locus_tag="VF633_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX8469944.1"
                     /translation="MFAKRESALAAAAGAAPQSKGPRLDFNSVVALFKRPPVAVGAAG
                     LVLLAAAALFLTVLGDPTAGTPSARAALHRETAEVAPTVAPTGLEAFGMDGFGAYQTL
                     AGDPNADPMSGAAGGDSLITLPDGATVAGGGSVITAPRRPAAPLLAAPIAGLSQPGPN
                     GPLPTIAADGRVPAQAYARPFRPNGKPRVALIVGGLGLNAVTTRAAIERLPAEVTLSF
                     VPYADGLQGWIDLARAQGHEVMLEIPMEPSGYPDNDPGPQTLLASATPDDITSRLSWL
                     LGRATGYFAVTNYLGDRFVTSDNGMSAFLSLLRQRGVAFIDDGSARRRPGAWARASAD
                     SVIDETQTPAAIVGRLNALEARAKATGAALGTGFSYPVTVEAAARWTAGLEGRGLQLA
                     PASAMTQRPGR"
     gene            complement(9135..10595)
                     /locus_tag="VF633_02430"
     CDS             complement(9135..10595)
                     /locus_tag="VF633_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8469945.1"
                     /translation="MRKLLLAGAAVLALGLSGMAVATQTPRNETFRMLQLFGDVLAVV
                     EQAYVVPVDNQKLIEAALAGMMSALDPHSNYLSPKGYGDLQERTSGEYSGVGLTITAE
                     GGLVKVISPMDNSPASRAGVQAGDVISAIEGQNASGMSVSDVSNKLRGAMGTSVTVTF
                     LRDGEEARDVVLTREVIKVESVTGRLEGDFGYIRVSTFNENTGRELTQVITRLKTEKP
                     GIKGFVLDLRNNGGGLLTAAIDVSDAFLERGEIVSQRGRKPDQIERYAAKPGDLTNGL
                     PLVVLINYGSASASEIVAGALKDQERATMVGLTSFGKGSVQTVIPLGGGKDGALSITT
                     ARYYTPSGRSIQKIGIEPDLEVARSEAEGRIVSRSSFIYSEAAYATALDASIGAERKG
                     AHTPHEAPGPDFDKEKDYQLERALDVLRAGGDLTKLAAAPTGIVISDGSTPTVTVAAN
                     DDTPDAAARPVDADAKPTTTPEVTPEATPTPQQQRR"
     gene            complement(10796..11854)
                     /locus_tag="VF633_02435"
     CDS             complement(10796..11854)
                     /locus_tag="VF633_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8469946.1"
                     /translation="MALAATPAASLRVPQENGELAGLQSEFRDEQVRARRLRADAADA
                     AAEIVNLDRQLASLHRAQNHDDLQMAAQRGRLNELSRREAVLLVALAKDRAQQGRLLS
                     ALQMMSRDPPPPLLIPADKAVDTVRAAILIRAITPELQRRTRTLVDQQTEIARIRRLA
                     VLSSEQLMTTESAQGDRRGEIESLSARKTSLVAVLRADAARSERAAKVLEARIRSLGG
                     EIQPLSSGEADAEAVVTRLPGGRSRLTPPTPGQPEATFGRGSSGWRWRPGARTIVAPS
                     NGVVDYAGPLSGWGEVVILDLGPGWRAVIAGLDTVSVEAGGRVTDGQALGRSSEGGEI
                     YFELRRDERPVDPGPWLR"
     gene            complement(11899..12363)
                     /gene="rlmH"
                     /locus_tag="VF633_02440"
     CDS             complement(11899..12363)
                     /gene="rlmH"
                     /locus_tag="VF633_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX8469947.1"
                     /translation="MKVSIIAIGKPGRGPEAALATDYAQRTSLAGRALGLGPLDLIDL
                     DAKKTGKAPEAELLLAAAEGAHLIACDERGKTYSSRAFADHIALLRDRGERKLVFVIG
                     GADGLDPSVLAAAGSTLAFGPQTWPHALARAMLAEQLYRAVTILAGSPYHRD"
     gene            complement(12511..12945)
                     /gene="rsfS"
                     /locus_tag="VF633_02445"
     CDS             complement(12511..12945)
                     /gene="rsfS"
                     /locus_tag="VF633_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX8469948.1"
                     /translation="MDEFAPIHSEDGDSDSGFDGPRAALPVGATALENAILGKLDDDK
                     AQDIVLIDLRGKSPMADTMIVASGRSHRHVGALADHLLRTLKEQGLGKAKVEGLPHCD
                     WVLVDAGDVIIHLFRPEVRMFYNIEKIWAVDSAHRTSTDIAV"
BASE COUNT         2303 a         4331 c         4281 g         2255 t
ORIGIN      
        1 gaccatgatc tcgttggggc cgaaatgcat ggtgcgggcg tcgttcagcc cctccacgcc
       61 gggctcggcc gtagcgatcg cctttatccc ctcgcgcgta gcgggatctg cggcttcacc
      121 ggtcagaagg ctctggctct cggaggccag aaagaatgcg gtgaccgcca ggatcagtcc
      181 gatggccacc gaggcgaccc cgtccagcac cggcatgtcg aacacctggc tgcatacgac
      241 ccccaccagc gccactacca gaccggccaa cgcggccgtg tcctcgaaca gtacggtgaa
      301 gacagtgggg tccttggagg tgtggaccat gccaaggaag ggccgcccgc cgcgctcctt
      361 gttgaaagcc ctgaacgcga tcgtccacga tgccccttcg aacaccatgg acaggccgag
      421 gatcagataa ttgacccacg ggttctctat cggctcaggg ttctggatgc gctcgatgcc
      481 atgagtgatg gagatgaccg cgccgatgcc gaagatcagc accgccacga cgaaggtgta
      541 gaaatagagc tgcagcccgt ggccgaacgg atggttctcg gtgggcggac gcgcggcgcg
      601 tttcagccca atgagaagaa tgacctggtt ccccgtatcc accgccgaat ggatcgcctc
      661 ggacatcatc gccgccgagc cggtgaacac tgcagcgatc agcttggtcg cggcgatggc
      721 cagatttccg gcgagcgcag cgaatatgac tttcttgttg gtcgatgaag ccatgatgtc
      781 tttcggagtg tgaggctctc ccgggctgag gcggaaggtc gcaggctcgc aagcgttccg
      841 gctccgatca ggttcctgcg tcgatcacga cttgttgcga gcaggaaaac cccatgctat
      901 caggcgcccg gcttcctagg ggcgtttcat acgaaacggc tccttgtgtg ctttcctaag
      961 cattacaggc ccttgaccgg cgtggactcc acggccgggc gcgaccacaa aagcttgacc
     1021 ggacggattc gacccttggc agacgatttc aggacgacag aagtcggcga gcgctatgca
     1081 cgggcgctgt tcgacctcgc tctcgagaca ggaaaactcg acgccgtgcg cgccgatctg
     1141 aagtccttga aggccgcttg gctggaaagc gcagacctgc gccgcctcgc cacctcggcc
     1201 attatcagcg ccgacgatca ggccaagggt cttgtcgcca tcgcggccaa ggcgaagttc
     1261 gagaccacaa cccggaattt cctcggcctg ctggcccaga acggccgcgc ccgtgatctg
     1321 gccgccgtca ctaccggctt cgaagccctg tacgccaagc acgcgggcgt cgtcgccgcc
     1381 gaggtcgtct cgacccagga actgacggtc gtccaactga cgcacatccg caacgccctg
     1441 cgccaagcct tgggcaaggc gcccgaaatc accacccgga tcgatccgtc gatcctgggc
     1501 ggtctgaagg tgaaggtcgg ctcgaagctg ttcgacgcct cgctgaagac caaactcgac
     1561 cagatgaagt tcgccctccg gcgcgcttga gacccctatt caacgaatga ctgccgaacg
     1621 catcctccct taacaggggc cgttcgcacc tgaagacgaa agccagagag ccagaccatg
     1681 gacatccgcg ccgccgagat ttcggccatc ctcaagtcgc agatcgccaa tttcggcgtt
     1741 gaagccgacg tgtccgacgt cggcagcgtg ctgtcggtgg gcgacggcat cgcccgcatc
     1801 cacggtctgg acaatgtcca ggccggcgaa atgatcgagt tcccgaaagc cggcgtgaag
     1861 ggcatggccc tgaacctcga gcgcgacaac gtcggcgccg tgatcttcgg cgccgatgcg
     1921 cagatcgccg agggcgacga agtccgtcgt ctgggcgaga tcgtggacgt tcccgtcggc
     1981 aagggcctgc tgggtcgtgt cgtcaacccg ctgggcgagc cgatcgacgg caagggcccg
     2041 atccagttca ccgagcgccg ccgcgtcgac gtcaaggctc cgggcatcat cccccgcaag
     2101 tcggttcacg agccgatgca aaccggcctg aaggccatcg acaccctgat cccggtcggc
     2161 cgtggccagc gtgagctgat catcggtgac cgtcaggtcg gcaagaccgc cgtcgccatc
     2221 gacaccatcc tgaaccagaa ggtcaccaac gccgctgcga catcggaagg cgacaagctt
     2281 tactgcatct acgtcgccat cggtcagaag cgctcgaccg tcgcccagat cgtcaagact
     2341 ctcgaagagc gcggcgctct ggaatacacc atcgtcgtcg ccgccacggc gtcggagccc
     2401 gctccgctgc agttcctggc cccgttctcg ggtacggcca tgggcgagtt cttccgcgac
     2461 aacggcatgc acgccctgat cgtttatgac gatctctcca agcaagccgt ggcttatcgc
     2521 cagatgtccc tgctgcttcg ccgcccgccc ggccgtgaag cctatcccgg cgacgtcttc
     2581 tacctgcaca gccgcctgct ggagcgttcg gccaagctga acgaagacaa cggttccggc
     2641 tcgatgacgg ccctgccgct gatcgaaacc caggccaacg acgtctcggc ctatatcccg
     2701 accaacgtga tctcgatcac cgacggccag atcttcctcg agtccgacct gttctaccaa
     2761 ggcatccgcc cggccgtgaa cgtcggcatc tcggtgtcgc gcgtgggttc gtccgctcag
     2821 atcaaggcga tgaaacaggt cgccggttcg atcaagggcg agctggccca gtaccgggag
     2881 atggccgcgt tcgccaaatt cggttcggac ctcgacgtct cgacccagaa gcttctggcc
     2941 cgcggcgccc gcctgaccga actgctgaag cagccccagt actcgccgct gaccgtcgaa
     3001 gagcaggtcg tttcgatcta cgccggtacg cgcggcttcc tcgatgcgat cgccgtctca
     3061 gacatcggtc gtttcgagtc cgagctgctg gcccggatgc actcgaacca cgcaggcatt
     3121 ctggacgcca tccgcacgca gaaggccctg tccaaggatc tcgaagccga gttgaaggcc
     3181 gctatcgacg ccttcgccaa gaccttcgcg taagcctgac ctagagagcg agagaccgcc
     3241 cgatggccag tctgaaggaa atgcgcaatc ggatcgaaag cgtcaagtcg acgcagaaga
     3301 tcacgaaagc cctgaacatg gtcgccgcgg ccaagctcaa gcgcgcccag gagtcggctg
     3361 aaaacgcgcg cccctatgct cgcaagatgg ccgccgtcat cgccaatctg gcggcgggcg
     3421 tgtcgggcga ctctgcgccg aaactgctgg ccggcacggg ctcggacaag aagcaccttg
     3481 tagtcgtggc tacgggcgac aagggtctgg cgggtggttt caacaccaac gtcatccgcg
     3541 ccgcacgtga ccggatcaac tcgctgatcg ccaacggcaa ggacgtccgc atcatcgccg
     3601 tgggccgcaa ggtccgcgat ggtctgaccc gtctgttcgg cgacaagatc atccgcacct
     3661 tcgagctcac cgggcacaag gtcatcggca tggccaccgc cgagcccatc gccgagttga
     3721 tcgccgacga gttcgagtcc ggcaacgccg acgtcgtgac cctgatcttc agccgcttcc
     3781 agtcggtcat ctcgcaggtg ccgacgcccc gacagctgat cccggcggtc gtcgacggcg
     3841 ccgccgagcc gatcaacctg aacggcgcgg tctacgacta cgagccctcg gaagaggaaa
     3901 tcctcgagac cctgctgccg cgcaacatca agacccagat cctggccgcc ctgctcgaaa
     3961 atcaggccag cttcttcggc gcccagatgg gcgcaatgga caacgccacg cgcaatgcgg
     4021 gcgaactcat caccgccttg aggctgacct ataaccgcaa gcgtcaggct caaatcacca
     4081 ccgaactgat cgagatcatc gccggcgcgg aagcgctctg atcttccgat cctgacagca
     4141 catacgataa cggaccgcac ccgatgaccg acaccgtcgc caagaaaccc gccgcccccc
     4201 gcaagcccaa ggctccggcc gaggttgccg ccaccggtaa cgccgcctat acggccggca
     4261 ccggcgtggg caagatcgct caggtcattg gcgccgtcgt cgacgtcgag ttcgtcggcg
     4321 aactgccggc gatcctgaac gcgctggaaa ccaccaacac cgaccagaag acgggcgagc
     4381 ccttccgtct ggtgctggaa gtcgcccagc acctgggtga gaacatggtc cgcaccatcg
     4441 ccatggacac gaccgagggt ctgacccgcg gccagcccgt gaccgacacg ggcacctcga
     4501 tcctggctcc ggttggcccg ggcacgctcg gtcgcatcat gaacgtcgtc ggcgacccga
     4561 tcgacgaagg tggtccgatc ctgaccacca tgtaccgccc gatccaccgc gaggcgcctt
     4621 cgttcgaaga gcagtcgacc tcgtcggaaa tcctcgtcac ggggatcaag gtcatcgacc
     4681 tgatctgccc ctacaccaag ggcggcaaga ccggcctgtt cggcggcgcc ggcgtgggca
     4741 agaccgtgac catgcaggag ctgatcaaca acatcgccaa ggcctacggc ggttattccg
     4801 ttctggccgg cgtgggtgag cgcacccgcg agggcaacga cctgtatcac gagatgatcg
     4861 agtccaacgt gaacgtcgcc ggtggcggcg aaggctcgaa atgcgcactc gtctacggcc
     4921 agatgaacga gcctcccgga gcccgcgccc gcgtcgccct gaccggtctg gcccaggccg
     4981 aatacttccg cgatgaagag ggcaaggacg tccttctgtt catcgacaac atcttccgct
     5041 tcacgcaggc cggttcggaa atgtccgctc tgctgggtcg tattccttcg gcagtgggct
     5101 atcagccgac gctcgcgacc gagatgggca acctgcaaga gcgcatcacc tcgaccaaga
     5161 agggctcgat cacctcgatc caggccatct acgtccccgc cgacgacctg accgaccccg
     5221 cgcccgccgc ctcgttcgcc caccttgacg cgacgaccgt gctgaaccgc gacatcgccg
     5281 cccaggccat cttccccgcc gtcgacccgc tcgactcgac ctcgcggatc atggacccgc
     5341 tggtcatcgg tgaagaacac taccgcgtcg cccgttcggt ccaggaaatc ctgcagcagt
     5401 acaaggcgct gaaggacatc atcgccatcc tcggcatgga cgagctgtcc gaagaggaca
     5461 agctggtcgt ctcgcgcgct cgcaagatca gccgcttcct gtcgcagccc ttcttcgtgg
     5521 ccgagcagtt caccaacgcc cccggcaagt tcgtggcgct ggccgacacc atccgctcgt
     5581 tcaagggcat cgtcgccggc gaatacgacc acctgccgga agccgccttc tacatggtcg
     5641 gcgccatcga ggaagccgtc gagaaggccc agaagatggc ttcggaagcc tcttgatcat
     5701 ggctggaaag cttcagttct cgctcgtcgc ccctgaacgc gaggtcttca ccggcctcgt
     5761 cgatcaggtc gacgcgccgg gcgtcgaggg cgacttcggc gtcctgccgg agcacgctcc
     5821 gttcatgacc gccctgcgcg aaggcgtggt cacggtgttc gacggctcaa cccgtcgcgt
     5881 gttcgaggtc cacggcggtt tcgccgacgt gactccggct ggcctgacca tcctcgccga
     5941 acaggcttcc gaagtttcgg cgacctagcg gctcgtggcc gggccgggaa tgatcctgac
     6001 ccttgcctgg atcggggcgg tcgttagccc tttagtctgc gctttgttga tccggcggct
     6061 gcttcgcagt cgccggatta tttttgccgg agccttaaca gcaatcaccg cagcggcctg
     6121 ggttctcggg gtctgggcct ttctggtcga gcctgcgacc ctgaccgttc gccacgtcac
     6181 ggtcgaaagc gcgcaatggc gcggtccgcc gttgcgcatc ggtttgatct ccgacaccca
     6241 tgtggccgca ccccacacgg atgtccgccg ggtagagcat ctggttgcac ggatgaacgc
     6301 ccaacatccc cacgtcgtca tgctgctggg cgactatgca ggcggccacg aaccagcggc
     6361 cacgcgagca caccccgaac agtccgaaat cctgcgcggc atcgaggcgt tccgcagcct
     6421 gactgccccg ttcggtgtcc acggcgtgct gggcaaccat gactcatggt atgatgacgc
     6481 ggctatttcg ggagcgttgc gacgcgccgg agcgagcgta ctggacaacc gcgcggaacg
     6541 gatagagaga ccgggcgggg cattctggat tgccggtctg gcggatatcg agtccccgcg
     6601 tgatccgccg gatgttgcgg ccaccctcgc tcaggtgacg gacgacgcgc cggtcatcgt
     6661 gctcagccac tggccggatc ccttcgcgga agtccccggt cgggtcgcca tcacgctcgc
     6721 cggccacacg cactgcgggc aggtcaacct gccgctgttc ggccgtgtgg tgcatgcgtc
     6781 acggatgtcg aagcgttggg cctgcggact gtatgacgag ggcggacgca agctgttcgt
     6841 cactggaggg gttggcgtgt ccatcctccc ggtgcgattc cgcgcaccgc ccgagatcgt
     6901 aatcttgacc ctcaaatccg ctggagccca ttcatgagcc tcatcgacat cgccggtgta
     6961 ttcggcgtgc tgctgatcct catcgcttat gcaggggcga ccgcagggaa gctggatcca
     7021 aaacagtggc cggcgctggt gctgaacctc gtcggcgccc tgctgatcct gtggtcgctg
     7081 tcggtggact ttaatctgtc ggcggctctg atggagggcg catgggccat cgtggcggtg
     7141 ctgggattgg tgcgcttggt gatgaagcgc tgaacttcga tcccccgccg ggagtgggaa
     7201 ggatgtttca gcccacgaac ttcgcgaacg ccgcgacgac ctgttcgtag gctgcacgct
     7261 tgaacgggac gatcagatcg caggcttcgg acagatcgcc ccagcgccag tcgtcgaact
     7321 cgatctcttc gtgctggttg aggtcgatct cggcctcatc gccgatgaag cggtaggcga
     7381 accagatctg gcgctggcca ttccacggtt tgcgcttttc acgcagatgc tgggccatca
     7441 gttcggcggg gaagtcgtag atgatctcgc tttcggtgcg gcccagaagc tcgaccgaag
     7501 tcactccggt ctcttcctga agctcgcgca gggcggccgc ttcaaggtct tcgcccttgt
     7561 ccacgccgcc ttgaggaaac tgccagttat gcggaccgcc gacaccggca cgcttgccgt
     7621 accagacctg accgctggca ttgaacagca cgacgccgac gttggggcgg tagagggaga
     7681 ggtcttgcat ggggcgggtc cgagggcagt aggcagtagg cggcggtgcg gcgggggaga
     7741 ccggttcaac ccgacatctt ccctactgcc tactgcctaa cccctactgc ctctcttgcc
     7801 tatcttcccg gcctctgcgt catcgcgctg gccggagcca actgcagccc tcgaccttcc
     7861 agaccggcgg tccagcgggc ggcggcttcg acggtgaccg gatagctgaa gcctgtgccc
     7921 agagcggcgc cggtggcctt ggcgcgggct tcgagggcgt tgaggcggcc gacgatggcg
     7981 gcgggggtct gggtttcgtc gatgacactg tcggcgctgg cgcgagccca ggcgcccggg
     8041 cggcgacggg ccgagccgtc gtcgatgaag gcgacgccgc gctggcgcag caggctgagg
     8101 aaggcgctca tgccgttgtc ggacgtcacg aaccggtcgc cgagatagtt ggtcaccgcg
     8161 aaatagccgg tggcgcgacc cagcagccac gacaaccgcg aggtgatgtc gtcgggcgtg
     8221 gcggaggcca gcagggtctg cgggccggga tcgttgtcgg gatagccgct cggctccatc
     8281 gggatctcga gcatgacctc gtgaccctgg gcgcgggcga ggtcgatcca gccctgcagg
     8341 ccgtcggcat agggcacgaa gctcagcgtc acctcggcag gcaggcgttc gatggcggcg
     8401 cgggtggtga cggcgttgag gccgaggccg ccgacgatca gggccacgcg cggcttgccg
     8461 tttgggcgga acgggcgggc ataggcctgc gccgggacgc gaccgtcagc ggcgatggtc
     8521 ggcaggggac cgttggggcc cggctggctc aagcccgcga tcggcgcggc cagcagagga
     8581 gcggcaggac ggcggggagc ggtgatgact gagccaccac cggcgacggt cgcaccgtcc
     8641 ggcagggtga ttagggagtc gccgcctgca gcgccggaca tcggatccgc attagggtcg
     8701 ccagcgagtg tctgataggc gccgaaaccg tccatgccga aggcttcgag cccggtcggg
     8761 gcgacagtcg gtgcgacttc ggcggtctcg cggtgcagcg ctgctcgtgc cgagggcgta
     8821 ccggccgttg ggtcgccaag caccgtcagg aacaaggcgg cggcggcgag cagcaccagc
     8881 cctgctgcac cgacggcgac cggcggacgt ttgaaaagcg cgacgaccga attgaaatcc
     8941 agacgcggac ccttgctctg tggagcggcg ccggcggcgg cggcgagggc ggattctctc
     9001 ttggcgaaca tacgacgaca ggccgctcgg tcagggatcg cgacggcgat ccttctcaaa
     9061 tgacatattg gcgtgtggcc ggttaagaaa ccctaacgcc gcattcacca tccgcgctca
     9121 agaggcgagc ccgatcagcg gcgctgctgt tgtggcgtcg gcgtcgcttc cggcgtcacc
     9181 tcaggcgtcg tcgtcggctt cgcatcggcg tcgacaggac gggccgcagc gtcgggcgtg
     9241 tcgtcgttgg ccgcaaccgt cacggtcggc gtcgaaccgt cggagatgac gataccggtc
     9301 ggggccgccg ccagtttcgt caggtcgccg ccggcgcgca gaacgtccag cgcccgctcg
     9361 agctgatagt ctttttcctt gtcgaagtcc ggacccggag cctcgtgcgg tgtgtgcgcg
     9421 cccttgcgtt cggcgccgat cgaggcgtcc agcgcagtgg cgtaggcggc ttcggaatag
     9481 atgaagctgg agcgcgagac gatccggccc tcggcctcgg agcgggcgac ctcgaggtcc
     9541 ggctcgatgc cgatcttctg gatcgaacgg cctgacggcg tgtagtagcg cgccgtcgtg
     9601 atagacaaag cgccgtcctt gccgccgccc agcgggatca cggtctggac cgaacccttg
     9661 ccgaagctgg tcaggccgac catcgtcgcg cgttcctgat ccttcagggc cccggccacg
     9721 atctcggacg ccgaggcgga tccatagttg atcagcacca ccaacggcag gccgttggtc
     9781 aggtcgccgg gcttggcggc atagcgttcg atctgatcag gcttgcggcc gcgctggctc
     9841 actatctcac cgcgttcgag gaaggcgtcg gacacgtcaa tggccgccgt caacaagccg
     9901 ccgccgttgt tgcgcagatc gaggacgaag cccttgatgc ccggcttttc ggtcttcagc
     9961 cgggtgatga cctgggtcag ctcacggccg gtgttctcgt tgaaggtcga gacgcggata
    10021 tagccgaaat cgccttccag acggccggtg acggattcaa ccttgatcac ttcgcgggtc
    10081 aggacgacgt cgcgcgcttc ttcgccgtcg cgcaggaagg tcacggtgac cgaggtgccc
    10141 atggcgccgc gcagtttgtt ggagacgtcc gagacgctca tgcccgaggc gttctggcct
    10201 tcgatggccg agatcacgtc gcctgcctgc acgcctgcgc ggctggccgg gctgttgtcc
    10261 atcggcgaga tgaccttcac cagaccaccc tcggccgtga tggtcaggcc gacgcccgaa
    10321 tattcgccgg acgtgcgttc ctgcagatcg ccgtagccct tgggcgacag atagttggag
    10381 tgcggatcca gcgccgacat catgccggcc agcgccgcct cgatcagctt ctggttatct
    10441 accgggacca cataggcctg ctcgaccacg gccagtacgt caccgaacag ctgcagcatc
    10501 cggaaggtct cgttccgggg cgtctgggtg gcgacggcca tgccgctcag gccgagggcc
    10561 agcacagcgg cgccggcgag aagcagttta cgcattcggt ttctttcgcg gtcgtctcaa
    10621 aagcttcggg tccgaagcct acgggcgtcc ggtattgggg tcaggagcgc cgaagtcacg
    10681 acgaaatcgt ggcgccgacg gatgtggggg ataaaatcag cgtaacgccc ggaatgaaag
    10741 cgctttcgct cgccggagca gcctcttctg cggccacgct gggcgtttga ggcgcttatc
    10801 gtaaccaagg gccgggatcg accgggcgtt cgtcgcggcg cagttcgaaa tagatctcac
    10861 cgccctcgga cgatcggccc agcgcctgtc cgtccgtcac tcgcccacca gcttcgaccg
    10921 atacggtgtc cagtccagcg atgaccgcgc gccaacccgg gccgaggtcg aggatgacca
    10981 cctcacccca gccactcagc ggtccggcat agtccacgac cccgttcgac ggcgcgacga
    11041 tggtgcgggc acccggcctc cagcgccagc cgctcgaacc gcgaccgaag gtggcctcgg
    11101 gctggcccgg cgttggcggc gtcaggcgtg agcgaccacc gggcaggcga gtgacgacgg
    11161 cttcggcatc cgcctcaccc gaggacagag gctggatttc accgccgaga ctgcggatgc
    11221 gcgcctccaa caccttggcg gcgcgttccg accgcgcggc gtcggcgcgc aggacggcga
    11281 cgagggatgt cttgcgggcc gaaaggctct cgatctcgcc gcgtcggtcg ccctgagcgc
    11341 tctcggtcgt catcagctgt tcgctggaaa ggacggcgag gcggcggatg cgggcgatct
    11401 cggtctgctg atcgaccagg gtgcgcgtgc ggcgttgcag ctcaggcgtg atagccctga
    11461 tcaggattgc cgcgcgaacg gtgtcgacag ccttgtcagc ggggatcagc aaaggcggcg
    11521 gcggatcccg gctcatcatc tgcagggccg acagcaaccg cccctgctgg gcgcggtcct
    11581 tggccaacgc gaccagaagc accgcctcgc ggcggctcag ttcgttcagc cggccccgct
    11641 gggcggccat ttgaagatcg tcgtggttct gggcccgatg gaggctggcc agttgtcggt
    11701 cgaggttaac gatctcggct gcggcgtcgg cggcgtcggc cctcaggcgg cgggcgcgga
    11761 cctgttcgtc acgaaactcg gattgcagcc cggccagttc gccgttttcc tgaggcacgc
    11821 gcaggctcgc ggccggcgtc gccgccagcg ccatcgcaca gccgagaagg atcaggcgtt
    11881 tcaggggagc ggtccggttc agtcccgatg atagggtgat ccggccagga tcgtcacggc
    11941 ccgatacagc tgttcggcca acatcgcgcg ggccagcgca tgaggccagg tctggggacc
    12001 gaaagcgagc gtcgatccgg cggcggcgag cactgatgga tcgagcccgt cagcgccgcc
    12061 gatgacgaac accagctttc gctcaccgcg atctcgcagc agggcgatgt gatcggcgaa
    12121 cgcccggctg gaataggtct tgccgcgttc atcgcaggcg atcagatgcg cgccctcggc
    12181 ggcggccagc aacagttcgg cctcgggagc cttgccggtc tttttggcgt cgaggtcgat
    12241 caggtcgagc ggcccgagcc ccagcgccct ccccgcaagg gaggtgcgct gggcatagtc
    12301 ggtcgcgagc gcggcttcag gtccgcgccc gggcttgccg atcgcaatga ttgaaacctt
    12361 caccctgaac tccgctcatc cccgcgaaag cggggaccca gttctgttgt gaagcatagc
    12421 gtatgggatg agggtccgtc gccagtcgtc atgatccgat cgcccaaagc gctgggtccc
    12481 cgctttcgcg gggatgagcg gaaactgttt ttagacggcg atgtcggtcg acgtgcggtg
    12541 ggcgctgtcg acggcccaga tcttctcgat gttgtagaac atccgcactt cgggccggaa
    12601 caggtggatg atcacatcgc cggcgtcgac aagaacccag tcgcagtgcg gcaggccttc
    12661 gaccttggcc ttgccgagac cctgttcctt gagcgtacgc agcaggtggt cggccagagc
    12721 gccgacgtgg cggtgcgaac ggccggaagc cacgatcatg gtgtcagcca tcggcgattt
    12781 gccgcgaagg tcgatcagga cgatgtcctg ggccttgtcg tcgtcgagct tgccgagaat
    12841 agcgttttca agcgcggtcg cgccgaccgg cagcgcagcg cgcggtccgt cgaagccaga
    12901 gtctgaatcc ccgtcttcgg agtggatggg cgcgaattcg tccatctcgt gggccgtgtt
    12961 ggaaacggct tcgtcttgcg tatcgtacgc ggtcgagggg gtcagcggaa agctccgggg
    13021 cggtgactaa agaaggtctc tagcacagat ggtccccaag gtcacccgac tcaagcagag
    13081 ccgtggttct ccgttcgcgt aacggtcgat tcccgcgcct tgacccgcag cgccgtcgat
    13141 gaactgagat tcagcggagc cgtcagatag
//
LOCUS       DASVTM010000088         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_593229,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000088 DASVTM010000000
VERSION     DASVTM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593229"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..764
                     /locus_tag="VF633_02450"
     CDS             162..764
                     /locus_tag="VF633_02450"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8469949.1"
                     /translation="MALLDWMTDATGPVVAGNGVMLRPPRVGDYQAWADLREASRDYL
                     QPWEPAWPDDDLTRAAYRRRLTVYAREMELGNAWPFFILDPSGRTLYGAITLSNIRRG
                     VADTGTLGYWIGLPWSGEGHGTAAVQALARHAFGQLKLHRLEASCLPSNAASRRVLEK
                     SGFRLEGEAKAYLKINGVWADHLLFGLLSEDAERADARST"
     gene            780..>2107
                     /locus_tag="VF633_02455"
     CDS             780..>2107
                     /locus_tag="VF633_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8469950.1"
                     /translation="MTPTRSRTLAWDATTAIEALAAADTALWIWTPSDDTLRFTGATR
                     PLGLGPLAPECSGAAFVAVTLPQDRSLAESILTPREEGSEIAVRLRMRGSETCLWRGV
                     WLEDGLRAAGVVALETKFAGSNKDMLTGLLDRRAFLSRASDVLAVPGDYDLVVADVDR
                     LRRLNEALGHERTDLVLSALGSRLAAAFSDQASPARIGEDEFAVLLPRGVSNASDRVR
                     DALEQPLRVAGFDIYPTVSIGSVACEGGADAPDPAELLRRVELAVSHAKGAGRGGASA
                     YGRALESDSLSRLALEADLRNAFVRGEIEPFFQPIVNLNTGAVAGFEALARWRHPKRG
                     LVPPDEFLGLTEEMGLMNDLGLMMMTQSARQLAEWLHRHPRAGKLFCSVNLSVGEIER
                     ADLVEDVQRVIQETGLPKGALKLEVTEGDIMRDTTRAAVILQQLKDVGASL"
BASE COUNT          321 a          686 c          730 g          370 t
ORIGIN      
        1 ccgaaggcga tcggtcttgc gaaagggtga ggggttagtc tcaccggtag acgccaaccc
       61 ctcacccttc ggctacacgc atcgctgcgc tctgcgagcc tcaagtcctc tcccgccggg
      121 agagggttca caaagcggcg cccctcacgc taatatttcc tatggccctt ctcgactgga
      181 tgacggatgc gaccggtccc gtggtggctg gaaacggcgt gatgctgcgg ccgccccgag
      241 ttggcgacta tcaggcctgg gcggacctgc gcgaggcttc gcgggattat cttcagccgt
      301 gggagccggc ctggccggac gacgacctga cccgcgccgc ttaccgccgt cgcctgaccg
      361 tctatgcgcg tgaaatggag ctcggcaacg cctggccatt cttcatcctc gatccgtcgg
      421 ggcggaccct gtacggggcg atcaccctgt ccaatatccg gcggggcgtc gccgataccg
      481 ggaccctggg ctactggatc ggcctgccct ggtcggggga gggacatggg acggcggcgg
      541 tgcaggcgct cgcacgccac gcgttcggcc agctgaaact gcaccggctc gaggcctcct
      601 gcctgccctc caacgccgca tcgcgccgtg tgctggaaaa atccggcttc aggctcgagg
      661 gtgaggcgaa agcttatctt aaaattaacg gcgtttgggc agatcatctt ctgttcggcc
      721 tcttgtcaga ggacgccgaa cgggccgacg caaggtccac ctgaaggaag agctcgcccg
      781 tgacacccac gcgatccaga accctggcct gggacgcgac gacggcgatc gaggcgctgg
      841 ccgccgccga tactgcgctg tggatatgga ctccgtccga tgacacgctg cgcttcaccg
      901 gagccacccg cccgctaggt ctgggcccgc tcgctccaga gtgttcgggc gccgccttcg
      961 tcgccgtcac cctgccccag gaccggtcgc tggccgagag catcctgacc ccgcgcgagg
     1021 aaggctccga gatcgccgtg cggctgcgaa tgcgggggtc cgagacctgc ctgtggcgtg
     1081 gcgtatggct cgaagacggg ctgcgcgccg cgggggttgt cgcgctggaa accaaattcg
     1141 cgggctccaa caaggatatg ctgacgggcc tgctggaccg gcgcgccttc ctttcacgcg
     1201 cgtccgacgt gctggcggtt ccgggggact atgatctggt ggtcgccgac gtcgaccggt
     1261 tgcgccggtt gaacgaggct ctgggccatg agcgcaccga tctggtgctc tcggctctgg
     1321 gctcgcgctt ggccgccgcc ttctcggatc aggcctcgcc ggcccggatt ggcgaggatg
     1381 agttcgccgt gctgctgccc cgtggggtca gcaacgcctc cgaccgggtg cgtgatgcac
     1441 tggagcagcc gttgcgggtc gcgggtttcg acatctatcc gaccgtctcg atcggttctg
     1501 tggcttgcga gggcggggcc gacgcccctg atccggccga gctgctgcgc cgcgtcgaac
     1561 tggccgtgag ccatgccaag ggagccggac ggggtggcgc ctccgcctac gggcgagcgt
     1621 tggaaagcga ttccctgagc cgtctggctc tggaggccga cctgcgcaac gccttcgtgc
     1681 gcggtgagat cgaacccttc ttccagccga tcgtgaacct caacaccggg gccgtcgccg
     1741 ggtttgaggc gctcgcccgc tggcgtcacc ccaagcgcgg tctggtgccc ccggacgagt
     1801 tcctcgggct gaccgaggag atggggctga tgaatgatct gggtctgatg atgatgacgc
     1861 agtcggcgcg gcagcttgca gagtggttgc accggcatcc gcgcgcgggg aaactcttct
     1921 gcagcgtcaa tctgtcagtc ggagagatcg aacgggccga tctggtcgag gatgtccagc
     1981 gggtgatcca ggaaaccggc ctgcccaaag gtgcgctgaa gctggaggtc accgagggcg
     2041 acatcatgcg cgacacgacc cgggcggccg tgatcctgca gcagctcaag gacgtggggg
     2101 cgtcgct
//
LOCUS       DASVTM010000089         5689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_646284,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000089 DASVTM010000000
VERSION     DASVTM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646284"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..915)
                     /locus_tag="VF633_02460"
     CDS             complement(277..915)
                     /locus_tag="VF633_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX8469951.1"
                     /translation="MTAIRFAPRFAALATVSAVAMLAVSACGKKEEAAPVPAEPMAPA
                     AATAPATTDPMVGGAPMSPNATIVANASKAANLTTLVSAVQAAGLAETLSATGPFTVF
                     APDNAAFEKIPATTRESLMTPAGNTALTKILTYHVVPGRLTAADLTAQAQANGGRAEL
                     TTVQGDTLTVMVNADGSVTLTDAKGGTSKVTQADVMQSNGVVHVIDTVVMPK"
     gene            complement(1030..1530)
                     /locus_tag="VF633_02465"
     CDS             complement(1030..1530)
                     /locus_tag="VF633_02465"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX8469952.1"
                     /translation="MISDRKAAFAPVIDEQTRVLVLGSLPGDASLKAQQYYAHPQNGF
                     WRLMGGVIGCDLTALPYPERLDALKMAGVGLWDVIASAERPGSLDAAIRSPEAADLRS
                     LVARLPMLKAVAFNGGTAAKSGRRVLAGIEGLTLIDLPSSSPAYTRPISEKAEGWARI
                     GPMLHG"
     gene            complement(1665..2963)
                     /gene="purB"
                     /locus_tag="VF633_02470"
     CDS             complement(1665..2963)
                     /gene="purB"
                     /locus_tag="VF633_02470"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX8469953.1"
                     /translation="MITRYSRPEAAAIWSSETKYKIWFEIEAHAATKMAELGVIPVEA
                     AEAIWAKGENAAWDSDRIDEIERTTKHDVIAFLTHVSETVGEEARFLHQGMTSSDVLD
                     TCFAVQLARSSDLLIAGVDRVLTALETRAKEHKYTPTVGRSHGIHAEPVTFGLKLAGY
                     HAEFQRAKRRLITAKEEIATCAISGAVGTFANVDPAVEEYVAGKMGLQVEPVSTQVIP
                     RDRHAAFFAALGVVASSVERLAIEIRHLQRTEVLEVEEFFDKGQKGSSAMPHKRNPIL
                     TENLTGLARLVRSAVTPAMENVALWHERDISHSSVERGIGPDATCHLDFALNRLAGVM
                     ERLLVYPENMQKNLDKLGGLVHSQRVLLALTQLGLSREDSYAAVQRNAMKVWRGEGNF
                     LDFLKEDAEVIVPDADLEALFDLGYHTKHVDTVFQRVFGS"
     gene            3098..3739
                     /locus_tag="VF633_02475"
     CDS             3098..3739
                     /locus_tag="VF633_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8469954.1"
                     /translation="MELIIGPRRFSTWSLRPWLVLKHCTDDFTVRELDFNTAEGHAEM
                     LSVSPSGLVPVLKVDGETIWDSMAISVWCAERYPEAGLWPRDAHARWAARSAACEMHA
                     GFPALRKHCTLDPDHLQVGPSRSSPPESEAVAVEFRRLVSLWREMRERFGAGGTYLFG
                     EWSVTDAFFTPVASRVRHYQIDLTAYGDVDGIARAYVETLLQQPDFLDWEASA"
     gene            3871..3996
                     /locus_tag="VF633_02480"
     CDS             3871..3996
                     /locus_tag="VF633_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469955.1"
                     /translation="MTAPVEPRPAQDSPRQPTLRSVALTLLVGCCLCASLIASLF"
     gene            complement(4013..>5689)
                     /locus_tag="VF633_02485"
     CDS             complement(4013..>5689)
                     /locus_tag="VF633_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8469956.1"
                     /translation="GFASGAALAEETPSSESAAVAATDSAEGVAYEGASSEGGPTALS
                     SADRLSYTTAFDALRRGDLELARASARQANDRVLLGQVEFERLFHPDHVTTYEELAAW
                     LEDYSDLPCAQRAYALALKRRPDGAAEPKRPAGVSGRTWSSVVEAGGGSTEDDPAKAA
                     RVAYNHDDLSTAYTLGIQIGDWWTAGLSAYRLGQFNESMQAFERVAADPTEDPWVRAG
                     AGVWASKAASQSGRQDRINEFLNLAARWPATFYGQVAMRQLGREPAIEGMGPRAYASL
                     QQANYQPARSVGDINPRDLNAFVQSDSRARRTVAYLEIGRRTDAQDELRAGLRTAVTE
                     TARRMWVGLADAIGPRIGGSGPDVTHIDAARYATPVLQPDGGFTIEPALVYALARKET
                     DFNASARSGAGAYGLMQVMPTTAAEMTGDRSFVSKPDQLLDPAVNMRLGQTYVNRMFA
                     RPEFQGDILRAVASYNAGPGPMLAALRKLGPTPDPLLLIETIDVPQARDYVEKVMAAY
                     WIYQRMFGKPLNTLDAVASGAGLISIGLDFVAPVPAPVEIASTLITAGGL"
BASE COUNT          986 a         1780 c         1886 g         1037 t
ORIGIN      
        1 cgcaagatcg cgggcgactt caaggcctcg gccctgagcg tgtctgaagg tgagattcgt
       61 tcgaggatgg acgagtacgc ttcggtagcc cgggatcaga ttcgcacagg cgaataagct
      121 tccggaaggc tgcggtgagg acaaacgaaa agggccgcac tcgggggaaa gtgcggccct
      181 tcgttttcgg gggctggggg gatagcctca cccgaaactc tttgcgtctt gcgctcccct
      241 acaatgcacc cgagcgcatt cggttcaccc gaaaaatcat ttcggcatga cgaccgtatc
      301 gatcacgtgg acgacgccgt ttgactgcat cacatcggcc tgtgtgacct tcgatgttcc
      361 accctttgcg tcggtaagtg tgaccgaacc atccgcattc accatcacgg tcagagtatc
      421 gccctgaacc gtggtgagtt cagccctgcc gccgttcgcc tgagcctgtg cagtcaagtc
      481 ggcggcggtc agccggcccg gcacgacgtg gtaggtcagg atcttggtca gggcggtgtt
      541 gcccgctggc gtcatcaacg actcacgcgt cgtggccggg attttctcaa acgctgcgtt
      601 gtcgggtgca aagaccgtga acggcccggt ggcgctcagg gtctcggcca gaccggcagc
      661 ctgaacggcg ctgaccaggg tggtcaggtt ggcagccttg gaagcgttgg cgacgatggt
      721 ggcgttgggg ctcatgggcg cgccgccgac catcggatca gtcgttgcgg gcgctgtcgc
      781 ggcagccgga gccatcggct cggcgggaac gggagcggcc tcttccttct tgccgcaggc
      841 cgacacggcc aacatggcaa cggccgagac tgtggcgagg gcagcgaaac ggggggcgaa
      901 acggattgca gtcatgacgt cttctccaga ggccgcgctg tgggcggcta tggagcaggt
      961 actgtcgagc gagcgaaagg atgcatctgg ccgcgccaga acgttgccgc aggtcgcgtc
     1021 gcacctgaat cagccgtgca gcatagggcc gatccgggcc cagccttccg ccttttccga
     1081 gatgggccgc gtgtaggcgg ggctggagga agggaggtcg atcaaggtca gcccttcgat
     1141 ccctgccagc actcgccgac cactcttcgc tgctgtcccg ccattaaacg ccacggcttt
     1201 cagcattggc aggcgggcga caagactgcg caagtcggcg gcttcaggcg accgtatggc
     1261 cgcgtccaga ctgccgggac gttctgccga ggcgatcacg tcccaaagcc cgacacccgc
     1321 catcttcaga gcgtcgagcc gctcgggata gggcagggcg gtgagatcgc acccgatcac
     1381 cccgcccatc aatcgccaga agccgttctg cggatgcgcg taatactgtt gtgccttcag
     1441 cgaagcgtcg cccggtaagc tgccaagcac cagaacgcgt gtctgctcat cgatgaccgg
     1501 ggcgaacgcg gcttttctgt cagagatcat ctagcgagac gagcactgga atctcgtttg
     1561 ctctcgacaa aatcgtagcc acgagagcca gatcgtccat gcgctccagc ctggccttgt
     1621 tggcgttggc ttgcactttc tggacgaacc cgaccttcac tgttctagct cccaaatacc
     1681 cgctggaaca cggtgtccac gtgcttggtg tgatagccga ggtcgaacag ggcttcgaga
     1741 tcggcgtcgg gcacgatgac ctcagcgtct tccttcagga agtcgaggaa gtttccttca
     1801 ccgcgccaga ccttcatggc gttgcgctgg accgccgcgt agctgtcctc acgggacagg
     1861 cccaattggg tcagggccag caggacacgc tgcgagtgaa cgaggccacc aagcttgtcg
     1921 aggttcttct gcatgttctc gggatagacc agcagccgct ccatcacccc ggccagacgg
     1981 ttcagcgcga agtccagatg gcaggtggcg tcagggccga tgccgcgttc cacggacgaa
     2041 tggctgatgt cccgctcgtg ccacaaggcg acgttttcca tcgccggggt cacggccgaa
     2101 cggaccagac gggcgaggcc ggtcaggttc tcggtcagga tcgggttgcg cttgtgcggc
     2161 atggccgacg agcccttctg gcccttgtcg aagaactcct cgacttccag cacctcggtg
     2221 cgctgcaggt ggcggatctc gatagccagc cgctcgaccg acgaggcgac gacgccgaga
     2281 gcggcaaaga aggcggcgtg gcggtcgcgc gggatgacct gcgtcgagac gggttcgacc
     2341 tgcaggccca tcttcccggc gacgtattcc tcgaccgccg gatcgacgtt ggcgaaggtg
     2401 ccgacagcgc cggagatggc gcaggtggcg atctcttcct tggccgtgat caggcgacgt
     2461 ttcgcgcgct ggaactcggc gtgatagccg gccagcttga ggccaaaggt cacaggctcg
     2521 gcatggatgc cgtggctgcg gccgacggtc ggagtgtatt tgtgctcctt ggcgcgagtc
     2581 tccagcgccg tcagcacccg gtcgacgccg gcgatcaaca ggtcggagga tcgcgccaac
     2641 tgcaccgcaa aacacgtgtc cagcacgtcc gatgaggtca tcccctggtg caggaagcgg
     2701 gcttcctcgc cgacggtctc ggacacgtgg gtcaggaagg cgatgacgtc atgcttggtg
     2761 gtgcgctcga tctcgtcgat gcggtcgctg tcccacgcgg cgttctcgcc cttggcccag
     2821 atggcctcgg cggcctcgac cgggatcaca cccagttcag ccatcttggt ggcggcatgg
     2881 gcctcgatct cgaaccagat cttgtacttg gtctcggagg accagatggc ggcggcttcg
     2941 ggtcgggaat agcgcgtgat catgggcgcg gggtgtcggt gcaaccccct ccggagtcaa
     3001 gcggcgcttg tggctggcca gcctaaactt ggtctccgcc ggtggtgatc cggcccgagt
     3061 cggtgcatcg aaatgcaacg cttggcaagg atgcaccatg gaactgatca tcggaccgcg
     3121 ccggttctcc acctggtcat tgcgcccgtg gctggtgctc aagcattgca ccgatgattt
     3181 cacagtgcgc gaactggact tcaacaccgc tgaaggtcac gccgaaatgc tgtctgtctc
     3241 gccgagcggt ctcgtgccgg tgttgaaggt cgacggcgag acaatctggg actccatggc
     3301 catctcggtc tggtgcgccg aacgctatcc agaggcgggt ctctggccaa gggatgctca
     3361 cgctcgctgg gcggcgcgat cggctgcgtg cgagatgcac gcgggctttc cggccttgcg
     3421 caaacactgc acccttgatc ccgaccacct gcaagtcggc ccgtcccggt cttcacctcc
     3481 tgagagcgag gcggtagccg ttgaattccg tcgtctggtt agcctctggc gcgagatgcg
     3541 tgaacggttc ggcgcgggcg gcacctattt gttcggcgaa tggtccgtaa ccgacgcttt
     3601 cttcacgccc gtggcgtcgc gggtgcggca ctaccagatc gacctcaccg cgtacggtga
     3661 cgtagatggg atcgcccgcg cctatgtcga gacgttattg caacaacccg attttctcga
     3721 ctgggaagcc tccgcctgac ctgaaccctg cctcatttcg aggcttcaag gacggcggtt
     3781 gttaagtacg cccgatgacc tgcgtttaag acgacgtcgt tatcttgaaa tcaagccggg
     3841 gagaccgtcc ccgacacagg acctgaagac atgaccgctc cggtcgagcc gcgcccggct
     3901 caggactccc cgcgccagcc gacgctccgc agcgttgcgc tcaccctgct ggtcggctgc
     3961 tgcctgtgcg cctcacttat cgcgagcctg ttctagcaag tcgcgaaaag gcttaaagcc
     4021 cgcccgccgt aatcagcgtg gaagcaattt cgaccggtgc gggcactgga gcgacgaagt
     4081 ccagtccgat cgatatcaga cccgcgcctg acgccaccgc gtccagagtg ttcagcggct
     4141 tgccgaacat gcgctgatag atccagtagg cggccatgac tttttcaacg tagtcacgag
     4201 cctgcggaac atcgatcgtc tcgatcagca gcaagggatc cggggtcggt ccgagcttgc
     4261 gcaacgcagc cagcataggc ccggggccgg cgttgtaaga ggcgacggcg cgcaggatgt
     4321 caccctgaaa ctcgggccga gcgaacatcc ggttcacata ggtctgaccc agtcgcatgt
     4381 ttaccgccgg gtcgagcaac tggtcgggtt tcgacacgaa tgagcggtcg ccggtcattt
     4441 cagccgctgt agtcggcatc acctgcatca gcccatacgc gccggcgccc gatcgggccg
     4501 aggcgttgaa atcggtttcc ttgcgggcga gggcgtagac cagcgccggc tcgatggtga
     4561 aaccaccgtc gggctgcagc acaggcgtgg cgtaacgcgc tgcatcgatg tgggtgacat
     4621 caggcccgga gccaccaata cgtgggccga tggcgtccgc cagaccgacc cacatgcgac
     4681 gagcagtctc cgtcacggcc gtgcgcaagc cagcgcgaag ctcgtcctga gcatctgtac
     4741 ggcgtcctat ctcaagatag gcgaccgtgc ggcgcgcccg gctgtcggac tggacgaagg
     4801 cgttcaggtc gcgcgggttg atgtccccca ccgagcgggc gggctgatag ttcgcctgtt
     4861 gcaagctggc ataggcgcgt ggacccatgc cttctatagc gggctcgcgg cccagttggc
     4921 gcatggccac ctgaccatag aaggtcgcag gccagcgtgc cgccaggttc aggaactcgt
     4981 tgatccggtc ctgccgaccg gattgcgagg cagcttttga ggcccagacc ccggcgccgg
     5041 cgcgcaccca ggggtcttcc gtcggatccg ctgcgacgcg ctcgaacgcc tgcatcgact
     5101 cgttgaattg gccgagccga taggctgaca gaccggccgt ccaccagtcg ccgatctgga
     5161 tcccgagcgt ataggcggtc gacaggtcgt cgtgattgta ggcgacgcgg gcggccttgg
     5221 cgggatcgtc ctcggtcgag ccgccgccgg cctcgacgac gctcgaccat gtccggccgc
     5281 tgacgccggc gggacgtttc ggttcggccg caccgtcggg gcgccgcttc agggccaggg
     5341 cgtaggcgcg ctgggcgcag ggcaggtcgg agtagtcctc cagccaggcc gccagttctt
     5401 cgtaggtggt gacgtggtcg gggtgaaaca gccgttcgaa ttcgacctga cccagcagca
     5461 cccggtcgtt ggcctgacgg gcggaagcgc gagccaactc caggtcacca cgccgcaggg
     5521 cgtcgaaggc ggtggtgtag ctgaggcggt cggcggaact cagggcggtg gggccgcctt
     5581 cagaggaagc gccttcatag gcgacgcctt cagcggaatc cgtcgccgcg actgcggcgg
     5641 actcggatga aggcgtttcc tcggcaaggg ctgcgcccga ggcgaagcc
//
LOCUS       DASVTM010000090         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_701463,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000090 DASVTM010000000
VERSION     DASVTM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701463"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..705)
                     /locus_tag="VF633_02490"
     CDS             complement(85..705)
                     /locus_tag="VF633_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8469957.1"
                     /translation="MQIAVLTFDGFNELDSFIALGILNRMKAKGWGAHITSPTPEVTS
                     VNSVTVKRQRPLEFATEADAVLVGSGIKTREIARDAGILDCLKLDPARQIIGAQCSGT
                     LLLAKLGLLGGLPACTDLTTKPWVVEAGVEVIDAPFIAHGNVATAGGCMASQYLAAWM
                     IARMATLEDAEQAIHYVAPVGEKALYVGLALDAVRPFLPRNESRAA"
     gene            complement(855..1196)
                     /locus_tag="VF633_02495"
     CDS             complement(855..1196)
                     /locus_tag="VF633_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469958.1"
                     /translation="MSILAFAVAAALLAVQTPVPQTPAPQTPAPQDAQTAAQAPTASA
                     ANAQLTPAQLRSHNRTQAQQICSRRAPTGRRLEQATCVSREMAAAQSRAGQDEHNRVT
                     LENGWSKESGN"
     gene            complement(1318..1749)
                     /locus_tag="VF633_02500"
     CDS             complement(1318..1749)
                     /locus_tag="VF633_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469959.1"
                     /translation="MAKLATIAMACAAYLAPSGMTFAQSAAPITNRDVVGQWALVVTP
                     VEQQGRSITFKSSDGRQQLDFPLTITAQPNGRLTCVADGNPADCRIRDGDLVVVSSGG
                     GVRMTFTLTDRTRAGLSGTASLRVRLLPIGGPIGSVVMTRR"
     gene            complement(1845..>2286)
                     /locus_tag="VF633_02505"
     CDS             complement(1845..>2286)
                     /locus_tag="VF633_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8469960.1"
                     /translation="SAAVPTGPDYRMTSSTTANNNRPAANAPRGNLTASLNGGVAAPS
                     STRGAATPPNRSTPARPIPTRNAAGQWAVQVGAFRDEAVARNWLAEINRRFRTQFSSA
                     ERTVQSASGWYRSRFTGLTEANAQAACSVLSERRVTCAVIRPEA"
BASE COUNT          378 a          707 c          793 g          408 t
ORIGIN      
        1 ccatcccgat caaggcggca ccgaaggctt ggccgcgcgg gtgaacgccg cgcgggatcg
       61 gttgctgaag cggtagccga ccgttcaagc ggcgcgggac tcgttccggg gcaggaacgg
      121 gcggacggca tcgagcgcga gcccgacgta tagcgccttc tcaccgacag gggcgacgta
      181 gtggatcgcc tgctcggcat cctccagcgt cgccatgcgg gcgatcatcc aagcggcaag
      241 gtattgcgac gccatgcagc cgcccgcagt cgctacgttg ccgtgcgcga tgaatggcgc
      301 atcgatgacc tccacgccgg cttcgacgac ccacggcttg gtcgtcaggt cggtgcaggc
      361 cggcagtcct cccaacaatc ccagcttggc cagcaagagc gttccggaac actgcgcccc
      421 gatgatctgt ctggcggggt cgagtttcag gcagtcgagg atgccggcgt ccctggcgat
      481 ctctcgggtc ttgatcccgc tgccgacaag gaccgcgtcc gcctcagtgg cgaactccag
      541 agggcgctga cgcttgaccg tgacgctgtt cacggaggtc acttccggcg tcggtgaggt
      601 gatatgcgcg ccccagccct tggccttcat tcggttcaga atgcccaggg cgatgaacga
      661 gtccagctcg ttgaagccat cgaaggtcag gacagcgatc tgcacggcaa tgctccctta
      721 ggccaactgc ggcccatgtt cagcctagac cgggaagctt ggcacttgtc actgaatgca
      781 gtggctgggc agtggcaatc gacgttattg tggcggataa cgccaacgga agacgttcca
      841 ggcgagcggc gtaactagtt gccgctctct tttgaccagc cgttctcgag ggtgaccctg
      901 ttgtgctcat cctggccggc ccgcgactgg gcggccgcca tttcacgcga gacgcaggtc
      961 gcttgttcga gccggcggcc agtcggggcg cgccggctgc agatctgctg cgcctgcgtc
     1021 cggttgtgac tgcgcaactg ggccggggtg agctgcgcat tcgcagcgct cgccgtcggt
     1081 gcctgtgcgg cggtttgggc gtcttgaggc gccggagtct ggggcgcagg agtctggggc
     1141 acgggtgtct ggacggccaa taaggctgcg gcgacggcaa aggccaggat cgacatgaca
     1201 ggctccggtt gagttcgacc tatcatgcca ccgacaggag gattttgcaa tttcacggat
     1261 caatggtcgg cacgcgcacc tggcgcgcgt gatgacgcgc cgaggccgca gaccttgcta
     1321 acggcgcgtc atgaccacgg atccgatcgg tccgccgatc gggagcaggc gaaccctcag
     1381 actggcggtg ccggataacc ccgcacgcgt gcggtcagtg agcgtgaagg tcatccgcac
     1441 gccgcctccg ctcgaaacga caacaaggtc gccatccctt attcggcagt cggccggatt
     1501 accgtccgcg acgcaggtga gtcggccgtt gggctgggcc gtgatcgtca acggaaagtc
     1561 gagctgctgc cgaccgtcgc tggatttgaa cgtaatgctg cggccttgtt gctcaacggg
     1621 cgtgacgacc agcgcccatt gaccaactac gtccctgttg gttatcggcg ccgcgctctg
     1681 ggcgaaggtc atgccggaag gagccagata cgcagcacag gccatcgcga tcgttgccag
     1741 cttcgccatg acagggctcc agaacacctt ccggtagcat cagtcggcca cagtcagctg
     1801 tcacgtgaat ctttggacag ttcagggagc aggcgaggca gacctcacgc ctccggacgg
     1861 atcaccgcac acgttacccg gcgctcgctg agcacggagc aggcggcttg ggcgttggct
     1921 tcggtgaggc cggtgaagcg tgagcggtac cagccggatg ccgactgtac cgtgcgttcg
     1981 gcgctcgaga actgggtacg gaagcgacgg ttgatctcgg ccagccagtt gcgggctacc
     2041 gcttcgtcgc ggaaggcgcc cacctgcacc gcccactgac ctgcggcgtt gcgtgtcggg
     2101 atcgggcgag cgggtgtcga gcggttgggc ggtgtcgccg cgccgcgtgt cgaagacggg
     2161 gctgcgacgc cgccgttcag cgaggccgtg agattgcctc gtggcgcgtt ggcggcgggg
     2221 cggttgttgt tggccgtagt gctgctggtc atccggtagt cgggacccgt cggaaccgca
     2281 gcggaa
//
LOCUS       DASVTM010000091         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_593452,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000091 DASVTM010000000
VERSION     DASVTM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593452"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VF633_02510"
     CDS             <1..278
                     /locus_tag="VF633_02510"
                     /inference="COORDINATES: protein motif:HMM:NF022456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEX8469961.1"
                     /translation="ALGRLGAWGGGPDPTRLLPFLEQALMGPRARRAVAALGVCAFLA
                     FYIWAVISIGARLPDHPLVHLLFYGISGIAWGVPLLPLLSWAERKPK"
     gene            complement(455..1381)
                     /gene="bla"
                     /locus_tag="VF633_02515"
     CDS             complement(455..1381)
                     /gene="bla"
                     /locus_tag="VF633_02515"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HEX8469962.1"
                     /translation="MKRIHTAGAVALVTLAACSTAASQAPTSAHPDPLLQPIAPDYAR
                     QWLRPQAPVRVYGNTWLVGFEGLTIGLIKTSDGLILIDGAAPQAVRDIEANLTTLGFS
                     IRDVKYILSTEAHWDHSGGIAALARDSGAEVLAGREAVQSLLQGRSTEDDPQHGELAS
                     MPAIAGVRGVRDGETLTLGDVTVTARATPGHTPGSMSWTWRSCEDDLCKDVVFAASLN
                     PISADGYLYSTHPALSEGFRRAFARVRAMPCDILFSSHPSASGQDARHAAFVRGEQPN
                     PFVDSAACRAFADHYERKFDERLAREGTGSVD"
     gene            1485..2357
                     /locus_tag="VF633_02520"
     CDS             1485..2357
                     /locus_tag="VF633_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8469963.1"
                     /translation="MDRAQLPLNALRAFEASARHLNFTRAAIELCVSQGAVSHQVAQL
                     EARLDVRLFRRLPRGLALTSEGQALAPMLAETFDRIGATLDRYAGGRLQEVLTVGVVG
                     TFAMGWLLPRMDAFRRALPDIELRLMTNNNRVDLAGEGLDLAIRYGDGAWHGTHADAI
                     LDAPLTPLCAPAVAARLSEPVDLARETLLRSYRADEWPRWFEAAGVASPPLRGPVFDS
                     SAMMVAAAEAGVGVALAPASMFTRDLMNERLVRPFALDVSLGGYWLTRLRSRAETDAM
                     RAFRDWLETTALDH"
BASE COUNT          397 a          832 c          829 g          440 t
ORIGIN      
        1 tggctttggg tcggctgggc gcttgggggg gcggccctga ccctactcgc ctactacctt
       61 ttctggaaca agcgctgatg ggaccgcgag caaggcgagc ggtcgcggcc ctgggcgtct
      121 gtgctttcct ggccttctac atctgggcgg tgatatcgat cggcgcccgc ctccctgacc
      181 atccgctggt gcacctgctg ttttacggca tttccggcat cgcatggggt gtgccgctgc
      241 tgcccctgct cagttgggca gagcgcaaac caaagtagcg agcggcggaa ttgcaaaggg
      301 gtcgcttcga gcgacccctt caaccaaccc attcccccca cgaaacggtc ggagcgacag
      361 gcttcgaacc cgcgtccctt gaccccctgc ggcttcaagg cgcgccgctt cgattgagtg
      421 atgagggtca cccagcgagc accggccttc gcgctcagtc gaccgatcct gtgccctccc
      481 tggccagacg ctcgtcgaac ttgcgttcgt agtgatcggc aaaggcccgg caagccgccg
      541 aatcgacaaa gggattgggc tgctcgccgc gcacgaatgc cgcgtgacgg gcatcttgtc
      601 cggaagcgct cggatgggat gagaacagga tgtcgcaagg catggcgcgc accctggcga
      661 aggcccgtct gaacccttcg ctcagggccg ggtgggtcga gtagagatag ccgtcggccg
      721 agatcgggtt caggctggcg gcaaagacga cgtccttgca cagatcgtcc tcgcatgaac
      781 gccaggtcca gctcatgctg cccggcgtgt gacccggcgt ggcgcgcgcg gtgacagtga
      841 cgtcaccgag cgtcagggtc tcgccgtccc ggacgccccg cacgccagca atggcgggca
      901 tggaggccag ttcgccgtgc tggggatcgt cctcggtgct ccgcccctgc agcaggctct
      961 gaaccgcctc gcgaccggcc aggacctccg ccccgctgtc gcgcgccagc gccgcaatgc
     1021 cgccggagtg gtcccagtgg gcctcggtgc tgaggatgta cttcacgtcc cggatcgaga
     1081 agccgagggt ggtcagattg gcctcgatat cccggaccgc ctgcggcgcc gcgccatcga
     1141 tcagtatcag gccgtcagac gtcttgatca gaccgattgt cagtccctcg aagcccacca
     1201 gccatgtgtt gccgtagacg cggactggag cctgtggacg cagccattgc cgggcgtagt
     1261 ccggcgcgat gggctgcagc agcggatccg gatgcgccga cgtcggcgcc tgcgatgccg
     1321 ccgtcgaaca ggccgccagc gtaaccagcg cgaccgcgcc tgccgtatgt attcgcttca
     1381 tcgctctctc cgtgttgagg tctgatacgg agagtggtga atcgaagctg gccagacaaa
     1441 gcatcatatc tggacagagt catgaactca tctaatgcct gatcatggat cgtgctcaac
     1501 tcccgctcaa cgccctgcgc gccttcgagg cctcggcgcg ccacctgaac ttcacgcggg
     1561 cagccatcga gctgtgcgtt agccagggcg cggtcagcca tcaggtcgcg caactggagg
     1621 ccaggctcga cgtccgcctg ttccgccgcc tgccccgcgg gctggcgctg acgtccgagg
     1681 gacaggctct tgcgccgatg ctggccgaga cgttcgaccg tattggcgcg actctggacc
     1741 gttatgcagg cgggcgactg caggaggtgc tgacggtcgg cgtggtcggg accttcgcca
     1801 tgggctggtt gctgccaagg atggacgcct tccggcgcgc gctgccggac attgagctgc
     1861 gcctgatgac caacaacaat cgggtcgacc tggccggcga aggcctggac ctggcgatcc
     1921 gctatggcga cggcgcatgg cacggcacac atgccgacgc gattctggac gcgccgctga
     1981 cgcccctgtg cgcccccgcc gtcgccgccc gtctttcgga gcccgtcgac ctcgcccgcg
     2041 agaccctgct gcggtcctac cgcgccgacg aatggccgcg atggttcgag gcggcgggcg
     2101 tggcgtctcc gccgctgcgt ggcccggtct tcgacagttc cgccatgatg gtcgccgccg
     2161 ccgaggccgg cgtgggcgtc gctctggccc cagcgagcat gttcacgcgc gatctgatga
     2221 acgagcggct agtccgtccg tttgctctgg acgtatcgct gggtggctac tggctgactc
     2281 gtctgcgatc ccgcgccgag acggacgcca tgcgagcctt ccgcgattgg ctggagacga
     2341 ccgctttgga tcattagcgc agttcatggc tccgtccaga aatcggacct tgtctgcccc
     2401 ggtcggcaag cgacacagtc gcttccatgg aaaactcagg ggagaagacg ggctgaaagc
     2461 gagcgacctc catcagggcg ccgaaggctg caagagcg
//
LOCUS       DASVTM010000092         5441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_168482,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000092 DASVTM010000000
VERSION     DASVTM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168482"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VF633_02525"
     CDS             complement(<1..346)
                     /locus_tag="VF633_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469964.1"
                     /translation="MTDLAALSFSVDSRPVAQGTADLAAFGAQSTRTAGAMDDASRRT
                     RLSLVAIGSAADQTGASVQGIASRIASYVRGVLGSLDGAARGLADGVVGSISRIQGSV
                     NSIDARPVDRVGR"
     gene            523..795
                     /locus_tag="VF633_02530"
     CDS             523..795
                     /locus_tag="VF633_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469965.1"
                     /translation="MIECDLTNEGQVPVAGVNYTASFTEANRTVPWAEIVGRPTRISG
                     GIEPGETAPILLQGQYLSGRADPSRVRINLRITEAFDVNGDSIPPE"
     gene            887..1285
                     /locus_tag="VF633_02535"
     CDS             887..1285
                     /locus_tag="VF633_02535"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEX8469966.1"
                     /translation="MHSATHLKAIPVPPGHAIIERDLSVAGLAHHKVHAARFCEGQNQ
                     AVELVPDPQNAHDANAIQVWGTWTDIDGAHGELLGFVWREEAAKIARAGIVGAIQGRL
                     YKTFLEDDYVSIRFQITAPKEAAAIYKATR"
     gene            complement(1352..2434)
                     /locus_tag="VF633_02540"
     CDS             complement(1352..2434)
                     /locus_tag="VF633_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEX8469967.1"
                     /translation="MDFSQRVSDLAQKSRHAASYAQTEEATKMAVVVPFIQMLGFDTS
                     NLEEVIPEFVADVGIKKGEKIDFAIRVDGKLAILVEVKPITMSLGAAQYSQLYRYFGV
                     SEARLAILTNGREFWFFSDIAEPNKLDKSPFFVFDVQSYHESQVSELARFQKSIFAID
                     AILEAASNLSRVSKVAGYLKKQLAQPDDDFVRMVGRQVETRNLTKPVIDELRQTVQAA
                     LDEVIRDRIQDKLGVTFAQEAPIKVVLPAEAAEALAEADEALDRSEVETTSDELMAFM
                     IVRAIGSKTVPIERIAMRDAKSYCAVFMDDNNRKPICRFYFNAKSVKSIGLFDAEKAE
                     TRHQVSGPSDLYKYAGEIEAVVSAYA"
     gene            2614..2901
                     /locus_tag="VF633_02545"
     CDS             2614..2901
                     /locus_tag="VF633_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8469968.1"
                     /translation="MFTVQLLPEFEAWATKLRDDRAKARIADRLERIEEGNLGDHKVF
                     QGLIELRIDYGPGYRLYACRREAVVIILLCGGDKSSQDRDIKRAINLAKEA"
     gene            2902..3195
                     /locus_tag="VF633_02550"
     CDS             2902..3195
                     /locus_tag="VF633_02550"
                     /inference="COORDINATES: protein motif:HMM:NF044550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEX8469969.1"
                     /translation="MTLETIPFDAARLLNTPGAQAEYLALAVESGDPARVAKAFGTVA
                     RAHGMANLAEEIGITRQGLAKALGEGGNPTLATLLALTKALGFRVTVEPLTAA"
     gene            complement(3192..3521)
                     /locus_tag="VF633_02555"
     CDS             complement(3192..3521)
                     /locus_tag="VF633_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469970.1"
                     /translation="MKSGRRQWIARHHGEEVAATYDIDIGPLGWLVDLMEDAGAVRIV
                     PTGMGALTTGLGWPEIAAWVEGAGEQSLSPTFRRGVMLLSAHYAAQVEASREMACPAP
                     FDPGKAD"
     gene            complement(3578..3955)
                     /locus_tag="VF633_02560"
     CDS             complement(3578..3955)
                     /locus_tag="VF633_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469971.1"
                     /translation="MDLTRYDTREKAHEGVDVPLIIDGETIIGDDDEPVTFRLRGIAD
                     PDVHRVILGLRRGDSRTPAEVLENDMRLARVAVAGWSGNWSLAGRKVPYSREGIAEVF
                     AVPTIRRAVLAEVLEDSHFMNGS"
     gene            complement(3955..4416)
                     /locus_tag="VF633_02565"
     CDS             complement(3955..4416)
                     /locus_tag="VF633_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469972.1"
                     /translation="MPVQLNPSAGAKLYVSATAPATPNEAGFAALTWVQVKGLDQLGE
                     FGKSYEVGTFDSLDEGRLKYRSILDEGDIDASPADLPADPGQIIVKAAADAPRGGVGE
                     VISFRAEAENGAGVYGRCIVATWKRQFGGATGIVLRRMVAPIVAGSTVEFS"
     gene            complement(4494..4733)
                     /locus_tag="VF633_02570"
     CDS             complement(4494..4733)
                     /locus_tag="VF633_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469973.1"
                     /translation="MTAKAEPAPIKGATITHVERDGTHYPALVYKGDKPAKGSVIRAD
                     FGGVTYEARVHAVSDEGDGMLVETSGLTPIPDPTT"
     gene            complement(4743..5138)
                     /locus_tag="VF633_02575"
     CDS             complement(4743..5138)
                     /locus_tag="VF633_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator-like protein"
                     /protein_id="HEX8469974.1"
                     /translation="MNRAAIVATLRGPLYAANLGLPVAWPNVPAPGDAPRIDVAWADP
                     DRTDPTLAMDSPIERGRMILTAVTPADEGEDPGNVLGDQLRAAYPACTRLPGAGVLVL
                     LGVPLVRTGFRDGDEWRVPVVVPYTAYPA"
     gene            complement(5135..>5441)
                     /locus_tag="VF633_02580"
     CDS             complement(5135..>5441)
                     /locus_tag="VF633_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469975.1"
                     /translation="ARVVGTIPRDLGTLAASLQSTLTGSTSITSPGQGGWVMVAGAMK
                     AGDVARFAWGGPAAPYARAVHDGAKGVPGTFWIDSMVNRFPGAVAEAVAEAKAEIMG"
BASE COUNT          925 a         1825 c         1765 g          926 t
ORIGIN      
        1 ctcggccgac ccgatcaact ggcctagcgt cgatgctgtt gacggaccct tggatgcgcg
       61 aaatcgaccc gaccacacca tcggccaaac ccctggcggc gccatcaaga gagcccaaga
      121 cgccccgcac gtaagacgcg atccgtgagg cgatgccctg gactgacgca cctgtctggt
      181 cagcggccga gccgatggcg acaagggaaa gccgcgtgcg acggctggcg tcatccatcg
      241 ccccagccgt gcgcgtgctc tgcgccccaa acgcagcgag gtcggcggtc ccctgggcga
      301 cgggtcggct atcgacgctg aaacttaggg ccgccagatc ggtcatgcgg cttgtcctcg
      361 cgcaaacggc gcaccacgtc ctcccagcga cacagggagg tcacgatgcg ctacggttgg
      421 gtttggggct tggcgctagc gggtccgctg gctgctcaag aggtgacagt cggcgagtgc
      481 cgtcctgcgg gtggctacgg cctaaatcag ccgacatttg acatgatcga gtgcgacctg
      541 acgaacgagg gacaggtgcc ggtggcgggc gtgaactaca cggccagttt cacggaggct
      601 aaccgcacgg tgccttgggc cgaaatcgtc ggccgcccca cccggatcag cgggggcata
      661 gagccaggcg agacggcacc gatcctactc caaggccagt atctctccgg ccgcgccgac
      721 ccatctcgcg tcaggatcaa tctcaggatc accgaggcgt tcgacgtgaa cggggactcc
      781 atccctccgg agtgagtaga gccgtcgcgt cccgttccct acttggactc gttgacgagg
      841 gctctacccc tcaagagctt acccgagaca gagcggggag gcgcccttgc actcggctac
      901 tcatctcaag gccatcccgg ttcctccggg tcacgccatc atcgagcgcg acctgtcagt
      961 cgctgggctt gcccaccaca aggtccacgc ggctcgcttc tgcgagggtc agaaccaagc
     1021 cgttgagttg gtgcccgacc ctcagaacgc ccatgacgcg aacgcgattc aggtctgggg
     1081 cacctggact gacattgatg gagcacatgg cgaattgctc ggcttcgtct ggcgagagga
     1141 ggcggccaag atcgcgcgcg ccgggatagt cggggccatc caaggccgac tctacaagac
     1201 cttcctggag gacgactacg tgtcgatcag gttccagatc accgcgccca aggaagctgc
     1261 ggcgatctac aaagccactc gttagcgacg gtagggcgac cccgaagggc tgctcctagg
     1321 cttcgatcac agggctcacg actagcccgc ttcaggcata ggctgagacc actgcctcaa
     1381 tctcgccagc atacttatag aggtcgctcg gcccactgac ttggtggcgt gtttctgctt
     1441 tttctgcatc aaacaagccg atggacttca cggatttggc gttgaagtag aaccggcaga
     1501 tcggcttgcg gttgttgtca tccatgaaga cggcgcagta ggacttcgcg tccctcattg
     1561 cgatccgctc gatcggcacg gtcttcgagc cgatcgcccg cacgatcatg aacgccatca
     1621 gctcgtcgga ggtcgtctcg acctcgctcc tgtcgagcgc ctcatcagcc tcggccagcg
     1681 cttcggcagc ctcggccggc agtacgacct tgattggcgc ttcctgcgcg aaggtcacgc
     1741 cgagcttgtc ctggatgcgg tcgcggataa cctcgtcgag cgccgcctgg accgtctggc
     1801 ggagctcgtc gatcaccggc ttggtcaggt tgcgcgtctc gacctggcgg ccgaccatgc
     1861 gcacgaagtc gtcgtcgggt tgggccaact gcttcttgag gtagcccgcg accttgctga
     1921 ccctcgacag gttagatgcc gcctccaaga tcgcgtcgat ggcgaagatg ctcttctgga
     1981 accgggcaag ttcgctgact tggctctcgt ggtaggactg gacgtcgaag acgaagaacg
     2041 gcgacttatc gagcttgttc ggctcagcaa tgtccgagaa gaaccagaac tcccgcccgt
     2101 tggtcaggat ggcgagccgc gcctccgaca cgccgaagta gcggtagagc tggctgtact
     2161 gggccgcgcc cagcgacatg gtgatcggct tgacctccac taggatggcg agctttccat
     2221 cgacgcggat ggcgaagtca atcttctcgc ctttcttgat gccgacgtcg gccacgaact
     2281 cggggatcac ctcttcgagg ttcgaggtgt cgaagcccag catctggatg aacggcacca
     2341 ccacggccat cttcgtggcc tcttcggtct gcgcgtagct tgccgcgtgc ctagattttt
     2401 gggcgagatc gctgacacgc tgagaaaagt ccacggggca ctccgtaagc gagcagatgc
     2461 cgcgtagcta aagtgctgag aacacggcgc cgatcaagcg gaaactcctc tccttcggct
     2521 gaagagcggc cccgaaggac cgcccgtcag tctgcgaaaa ctatagttgc accggggatt
     2581 gaccccctgc ccatctgcaa ctatagtttc ttcatgttca ccgttcagct tctccctgag
     2641 ttcgaggcct gggccaccaa gctccgggat gaccgcgcca aggctcggat tgccgaccgc
     2701 cttgagcgga tcgaggaagg caacctgggc gaccacaaag tctttcaggg gctgatcgag
     2761 ctgcgcatcg actacgggcc gggctaccgg ctctacgcct gccgccgcga ggcggtggtc
     2821 atcatcctgc tctgcggcgg cgacaagtcg tcccaggaca gggatatcaa gcgcgcgatc
     2881 aatctcgcca aggaggccta gatgaccctc gaaaccatcc cattcgacgc ggcccgcctg
     2941 ctcaacactc ccggggctca agccgagtat ctggccctcg ctgtcgagtc cggcgatccg
     3001 gcccgtgtcg ccaaggcgtt cggcaccgtc gcccgcgccc atggcatggc gaacttggcg
     3061 gaggagatcg gtatcacgcg ccaagggctc gccaaggcgc tcggcgaagg cggcaaccca
     3121 accctcgcca cgctgctcgc gctcacaaag gccctgggct tccgcgtgac ggtggagccg
     3181 ctgactgcag cctagtccgc cttccccgga tcaaacggcg ccgggcacgc catctcccgg
     3241 ctggcttcca cctgggccgc gtagtgcgcc gacagaagca tgacgcccct ccggaacgtc
     3301 ggcgaaaggc tctgctcccc tgccccttcg acccacgccg cgatctcggg ccaacccagc
     3361 cccgtagtca gcgcacccat tccggtgggg acgatcctga cggcccccgc gtcctccatc
     3421 aggtccacca gccacccgag cgggcctatg tcgatgtcgt aggtagcggc aacctcctcg
     3481 ccgtggtgac gcgcgatcca ttggcgacgt cccgacttca agtctgtggg agcggactcc
     3541 agccacgccc tttgccgcag atgaagaacg gccccagtca ggagccgttc ataaaatggc
     3601 tgtcctccag cacctccgcc agcacggcac ggcggatggt cgggaccgcg aagacctcgg
     3661 cgatcccctc tcggctgtag ggcaccttcc ggcccgccag cgaccagttg ccggaccagc
     3721 cagccacggc cacgcgcgct aggcgcatgt cattctccag aacctcggcc ggggtccgag
     3781 agtcgcccct gcgcagcccc aggatgacgc gatgcacgtc tgggtcggca atgccgcgaa
     3841 gccggaacgt gaccggctcg tcgtcgtcgc cgatgatcgt ctcgccgtcg atgatgagcg
     3901 gcacgtccac gccctcgtgt gccttctcgc gggtgtcgta gcgggtcagg tccatcagga
     3961 gaactccacc gtgctgcccg caacgatggg ggccaccatg cggcgcagca cgatgcccgt
     4021 ggcgccgccg aactgccgct tccaagtcgc aacgatgcag cggccataga cgcccgctcc
     4081 attctcggcc tcggcgcgga aggagatgac ttcgcccaca ccgccgcggg gggcgtcggc
     4141 cgcagccttc acgatgatct gacccgggtc agccggaagg tcggcaggcg aggcgtcgat
     4201 gtcgccttcg tccaggatcg agcgatactt gaggcgtcct tcgtccaggc tgtcgaaggt
     4261 gcccacctcg tagctcttgc cgaactcgcc gagttgatcg agtcccttga cctggaccca
     4321 ggtaagggca gcgaagccgg cttcgttggg ggtagccggg gccgtcgcgg agacgtagag
     4381 ctttgcaccc gcggacgggt tgagctgcac aggcatgtgc ttggtccttt cagcggaggg
     4441 gagcgcgccg acgtgggtcg cgcgggatca tcagcgtcgg gcgccgaatg cggtcaggtc
     4501 gtgggatcgg ggatcggggt caggccagag gtctcgacca gcatcccgtc gccctcgtcg
     4561 ctgacggcgt ggacgcgcgc ctcgtaggtc acgccgccga agtcggcgcg gatgacactg
     4621 cccttggcgg gcttgtcgcc cttgtagacg agcgccgggt agtgggtccc atcgcgctcg
     4681 acatgcgtga tcgtcgcgcc cttgatgggc gcgggctcgg ccttggccgt catagctctc
     4741 tcctaggctg ggtaggcggt gtaggggacg acgacaggca cccgccattc gtcgccgtcg
     4801 cgaaagcccg tgcgcaccag cggcacgccc aggagcacga gcaccccggc gccgggtagg
     4861 cgggtgcagg ccgggtaggc ggcgcggagc tgatcgccga ggacgttgcc ggggtcctcg
     4921 ccttcgtcgg ccggggtcac ggccgtcagg atcatccgcc cccgctcgat ggggctgtcc
     4981 atggcaaggg tcgggtcggt gcggtcgggg tccgcccagg ccacgtcgat gcgcggggca
     5041 tcaccgggcg ccgggacgtt cggccaggcg accggcaggc caaggttggc ggcgtagagc
     5101 gggccgcgca gggtggcgac gatggcggcc cggttcatcc catgatctcc gccttggcct
     5161 cggccaccgc ctcagcgacc gcgccgggaa agcggttgac catgctgtcg atccaaaagg
     5221 tgccggggac gcctttcgcg ccgtcgtgaa ccgccctggc gtagggcgcg gctggtccac
     5281 cccaggcgaa gcgcgccacg tcgccggcct tcatcgcgcc cgcgaccatc acccagccgc
     5341 cttgtccggg agacgtgatc gacgtgctgc ccgtcagcgt ggattgcagg gacgccgcga
     5401 gcgtgccgag gtcccgaggg attgtcccga cgacccgggc c
//
LOCUS       DASVTM010000093         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_593676,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000093 DASVTM010000000
VERSION     DASVTM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593676"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2350
                     /locus_tag="VF633_02585"
     CDS             <1..2350
                     /locus_tag="VF633_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8469976.1"
                     /translation="GFGELEREASVWRLLPDWTSGLATGTDGSSIANLGDAVSAELSQ
                     GRMVAVDEVATDLWTAPQIASHIARNDQAFVAVPLMRAGRLRAFFNVSSARPRVWTAE
                     EVGLINEVAERAWVEIERTRAQAEVAESEARFRAIADTAPVLIWVTQQNGERAFVNQT
                     YVAYMGGDYESVRTADWRTYIHPDDQDRLVHESHAGEATGQPFSLEARYKNHAGQYRW
                     LKSFSRPRFGPAGQIIGFVGVAFDVTDAREAQALLAESEARFRTVADSAPALIWMTDE
                     RPGIVFTNKRYKAFFNVDPEQISDDRWTDFIHPDDIKVFSATFLRAFKARVRFEAVMR
                     VQHHTFGQRWLRSEGVPRFDSTGAFQGYVGASLDITDAKRAEDDLKRINELLEERVGE
                     ALAEKAKAEADLMHAQRMEAVGRLTGGVAHDFNNLLTVVIGALDMMLKSPDDAPRRKK
                     LGEAALAAARRGEGLTHQLLAFSRRQALRPEAVDLNALIMESEPLLRRAVGEAVEYKV
                     KLRRGGARVSVDPAQFEAALLNLMVNARDAVGSRSKGRITVQTMSCTVTTGEVLELPA
                     GRYVCVTVSDNGAGMDAATMERVFEPFFTTKAVGKGTGLGLSQVYGFARQSGGGVHIS
                     STLGRGTEIKLYLPPLKASEVVTPIDDRRDIEAIPSGRRLLLVEDDVGVAAVATELLT
                     GLGLEVEAAQSGPEALERLGAAPFDVMLSDIVMPGGMTGIELARVCAERFPLMPILLT
                     SGYAGDDVDEALADAPWPFLRKPYSGDQLRQALGELLPFTEG"
     gene            complement(2432..2794)
                     /locus_tag="VF633_02590"
     CDS             complement(2432..2794)
                     /locus_tag="VF633_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HEX8469977.1"
                     /translation="MRDLKQTGFSDRITTQQEAKKALLAKFKPKAAAPDPEFERLAEK
                     RAAEKEALRLQHEQAKAEQRLEKAEKEAARLATEAASQEAVDAEKRAARKERKQVSKE
                     DQKAARDARYAARKARQR"
     gene            complement(3044..3229)
                     /locus_tag="VF633_02595"
     CDS             complement(3044..3229)
                     /locus_tag="VF633_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469978.1"
                     /translation="MKRILTAAAVAVSAIALTACGHTIEQRAATGAIAGAVVAGPVGA
                     AVGGAAGTVVGQAQKPH"
     gene            3686..>4175
                     /locus_tag="VF633_02600"
     CDS             3686..>4175
                     /locus_tag="VF633_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8469979.1"
                     /translation="MFHSAQPRANPTVDRVARIRPVTAAAMFGGLVGLAVGCAYLGGS
                     VAKATTVRAQAERLEGATSAGFTEEALAAAAGGLDASALTIARRHDPYTVAGGAQRDR
                     QAELMTARLEQLRGEPADKALRRISLANDAARPFRMGNALDASRDLECLTQAAYYEAR
                     GEG"
BASE COUNT          745 a         1340 c         1324 g          766 t
ORIGIN      
        1 aggctttggc gagcttgaac gggaggcgag cgtctggcgg ctgttgccag actggacctc
       61 cggcctcgcg accggcacag acggatcgtc gattgcaaac ctcggtgacg cggttagcgc
      121 agagctatcg caaggccgga tggtcgcagt cgacgaagtg gcgaccgatc tgtggacggc
      181 gccgcagatc gccagccata tcgcgcgcaa cgatcaggct ttcgtcgctg tccccctgat
      241 gcgtgcaggt cgtttgcgcg ccttcttcaa tgtctcgtct gcccggcccc gggtgtggac
      301 cgctgaagaa gttggcctga tcaacgaagt cgccgagcgc gcctgggtcg agatcgaacg
      361 cacccgggcc caggccgagg tcgccgaaag cgaagcccgt ttccgcgcta tcgctgatac
      421 agcccccgta ttgatctggg tgacgcaaca gaacggtgag cgggctttcg tcaaccagac
      481 ctatgtcgcc tacatgggcg gcgactacga aagcgtccgc accgccgact ggcgaaccta
      541 catccacccg gacgatcagg atcgtctggt gcacgagtcc cacgctggcg aagccaccgg
      601 ccagccgttc tcgctggaag cccgctacaa gaaccacgcc ggtcaatacc gttggctcaa
      661 atccttctca cgcccccgct tcgggcccgc cgggcagatc atcggctttg tcggcgtggc
      721 gttcgacgtc accgacgccc gtgaggccca agcccttctg gctgagtctg aggcgcgctt
      781 ccgcactgtc gccgacagcg cgcctgccct gatctggatg accgacgagc ggccaggcat
      841 cgtcttcacc aacaagcgct acaaggcgtt cttcaacgtt gatccagagc agatcagcga
      901 cgaccgctgg acagacttca tccatcctga cgacatcaag gtcttttcgg cgaccttcct
      961 ccgggccttc aaggcacgcg tccggttcga agccgtgatg cgggtgcagc accacacatt
     1021 cggtcagcgc tggctgcgct ccgagggtgt gccacgcttt gattccaccg gggccttcca
     1081 gggctatgtg ggcgccagtc tcgacatcac cgacgcaaag cgggcggaag acgacctcaa
     1141 acgcatcaac gaactccttg aggaacgcgt cggagaagcg ctggctgaaa aagccaaggc
     1201 cgaggccgac ctgatgcatg cccagcgaat ggaagcggtg gggcggttga cgggcggggt
     1261 cgcccacgac ttcaacaatc ttctaaccgt ggtgatcggc gcgctcgaca tgatgttgaa
     1321 gtcgcccgac gacgcccccc gtcgcaagaa gctcggcgag gctgcgctgg ccgccgcgcg
     1381 ccgtggcgag ggtttgaccc accagctgct agccttctcg cgccgtcagg cgctgcggcc
     1441 cgaggcggtc gatctaaatg ccttgatcat ggagagcgaa cccttgttgc gaagggctgt
     1501 cggcgaggcg gtcgagtaca aggtcaagct gcgtcgtggc ggtgcgcgcg tcagcgtcga
     1561 tccagcccag ttcgaagcgg cgctgctcaa tcttatggtc aacgcgcgcg atgcggtggg
     1621 atcacgcagc aagggccgga tcacagtgca gacgatgagc tgtaccgtca cgaccggaga
     1681 agtcctcgaa ctgccagccg gtcggtacgt ctgcgtgacc gtctcggaca atggcgccgg
     1741 catggacgcc gcaaccatgg agcgcgtgtt cgaacccttc ttcacgacca aagctgtcgg
     1801 caaaggcacg ggtctcggtc tcagccaagt ctatggcttt gcgcgccaga gcggcggtgg
     1861 cgtccacatc tcatcgaccc ttggacgggg cacggagatc aagctctatc tacctccgtt
     1921 gaaggcttcg gaggtcgtta ccccgatcga cgatcggagg gacatcgagg caattccatc
     1981 aggacgacgc ctgctgctcg tcgaggatga cgtcggggtc gcggctgtcg ccaccgaatt
     2041 gctgaccggt ctgggcctcg aggtcgaagc cgcgcagtcc ggtccggaag cgctggagcg
     2101 gctgggggcc gcaccgtttg atgtcatgct cagcgatatt gtcatgcccg gtggcatgac
     2161 cgggattgaa ctggcccgag tctgcgccga gcggtttccc ttgatgccca ttctgctgac
     2221 gtctggctac gccggcgacg acgtcgacga ggctcttgcc gacgccccct ggcctttcct
     2281 gcgaaagcct tattcaggag accagttgcg tcaggcgctc ggtgaattgc tgcctttcac
     2341 cgaggggtga tctgaaattc gcgtgtcgcc cctgcaaacg aaagtcagcg gagcgagccc
     2401 cgccggcttg atctagccga gacctgtcgg tttagcgctg acgcgctttc cgggcggcat
     2461 agcgggcatc ccgtgcagcc ttctggtctt ccttggatac ctgcttgcgc tccttgcggg
     2521 cagcgcgctt ctcggcgtcc acagcttcct ggcttgcggc ctccgtagcc agacgggcag
     2581 cttccttctc ggccttctcc agacgctgct cggccttggc ttgctcgtgc tgcaggcgca
     2641 gggcctcctt ctcggcggcg cgcttttccg cgagacgttc gaattcgggg tcaggcgcgg
     2701 cggccttggg cttgaacttg gccaggagcg ccttcttggc ctcctgctgg gtggtgatac
     2761 ggtcgctaaa gccggtttgt ttcagatctc gcatgcgtag ggtcggttgt cctttaggcg
     2821 gttcaggtca cgcggcgtcg ccgggtgaca gcgtcgcgtc tatacgggcg ggctccaccg
     2881 aaatcaggcg agccaaggcg gtgcgacaaa gcggaaaccc gagccaaaag caaggtgcgc
     2941 cgtgacggaa cgaccgcgag gacgccgatc ctcccgggat ggcgcctctg cgatcacagc
     3001 cggatatagg cacttggctc gaaaagccaa cccgcacgat ggctcaatgc ggcttttgcg
     3061 cctgaccgac gaccgtgccc gccgcaccgc cgacggcggc tccgactgga ccagccacga
     3121 cggctccggc gattgcgccg gtcgcagcgc gttgctcgat ggtgtggccg caagcggtca
     3181 gtgcaatcgc ggaaactgcg acggcggcgg cggtgaggat gcgtttcatg ggtcaagctc
     3241 ccgaatggta aagcttaacg tctttcgatt gacgacggtt cctcaattca ctgtccaagt
     3301 caaaagcctt aaggagaacc taatgaagac aatagtttcc ggctggataa gttcgtctgg
     3361 aaatgcgttc accatcgttg agttgataaa gggaaaccca atacagtaat cgatcacctg
     3421 actaacacgg ctttgtcgta gagaggctca gcctcgacac gaatgccttc cggttgcgtc
     3481 aaattgctgc gccgcacctg ccgcttgcga ctcaaacaca ttggcgccat aaatggatgg
     3541 cttaaggcgc tgcttcgatg accggctgat cgccgatcgc cgatttacca tagccgacgt
     3601 gatgagcccc aaagagggac cctcgccggc ctgtgaagac gaccaagggg ccgcgtttcc
     3661 cgccctgctg aacaggacta cgcctttgtt tcattccgcg cagccgcgtg cgaacccgac
     3721 tgttgatcgc gtggcccgta tccggcccgt gaccgcagct gcgatgttcg gcggccttgt
     3781 gggactggct gtcgggtgcg cctatctggg cggctcggtg gccaaggcga cgaccgttcg
     3841 cgctcaggcg gaacgacttg aaggcgccac ctcggcgggc ttcaccgaag aggccctcgc
     3901 ggccgcagcg ggcgggctgg atgcaagcgc cctcaccatc gcccgtcgcc atgaccccta
     3961 tacggtggcc ggcggcgccc agcgcgaccg ccaggccgaa ttgatgaccg cgcgcctcga
     4021 gcaactgcgc ggcgagccag ccgacaaggc gcttcgccgt atcagcctcg ccaacgacgc
     4081 cgcccgcccg ttccgcatgg gcaacgcgct cgacgcctcg cgcgacctcg aatgcctgac
     4141 acaggccgcc tattacgaag cgcgcggcga aggcc
//
LOCUS       DASVTM010000094        21519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1019324,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000094 DASVTM010000000
VERSION     DASVTM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 21519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21519
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019324"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VF633_02605"
     CDS             <1..547
                     /locus_tag="VF633_02605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX8469980.1"
                     /translation="RNRPEDAPIGLIAVDALYSPVKRVAYRVEPTRLGQSLDFDKLVM
                     EVETNGAVSPVDAVAYASRILQDQLQIFITFDEPKKAVEASEGKPDLPFNPALLKKVD
                     ELELSVRSANCLKNDNIVYIGDLIQKTEGEMLRTPNFGRKSLNEIKEVLTSMGLSLGM
                     DVPNWPPENIEDLAKKFDDQI"
     gene            655..1071
                     /gene="rplQ"
                     /locus_tag="VF633_02610"
     CDS             655..1071
                     /gene="rplQ"
                     /locus_tag="VF633_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX8469981.1"
                     /translation="MRHGAAHRKLGRTSAHRTAMFANMAASLIKHEQITTTLPKAKEL
                     RPFVEKLVTLGKKGDLHARRQAISHVRDVTQVGKLFETIGPRYAERSGGYIRIMKAGF
                     RHGDNAPMAVIEFVDRDVDAKGLDSGPVYAVEGDEE"
     gene            1230..1700
                     /locus_tag="VF633_02615"
     CDS             1230..1700
                     /locus_tag="VF633_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8469982.1"
                     /translation="MTSPSLEIVSYRPDHAEAWRSLNTFWILAGGYAIEAKDELVLSD
                     PEGAILSKGGAIFIAELDGQAVGCCSLMAMEDGGYEVGKMAVDAVAHGRGLGERLLEA
                     CEAKAGSASAHRLYLETNAAQTHAIALYRRFGFVDLPAQPSPYARCNVWMEKRL"
     gene            1890..2816
                     /locus_tag="VF633_02620"
     CDS             1890..2816
                     /locus_tag="VF633_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8469983.1"
                     /translation="MKRPAPPPLNALRAFEAFARHGGMTAAGAELCVTHGAVSRQVRM
                     LQDVLGVVLVTGPRHRLALTDAGTALAHSLSGAFLAIDDAVATARVGPFREIGISCLG
                     TFGLKWLIPRLPGFLDAHPGIRVRMSESYAPADFKRDQIDGAIRICAPEQAPRGQEVT
                     PFMELQQGLVIAPHLIADDASVAAIARLPRLHSATFRESWSVWSELMGIDLPPACVER
                     EFGHNHSMVEAVAAGMGAAIAPWAFVAPDIRSGRLAAPFSFVARPGHQFVFLRPENRG
                     DPAVDAFRDWLVAEGAASPPPPSLPTYSFDRS"
     gene            complement(2795..3655)
                     /locus_tag="VF633_02625"
     CDS             complement(2795..3655)
                     /locus_tag="VF633_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469984.1"
                     /translation="MLTDAISSEAYRFSKNRMAVFWSTMFLPVVLLIIGSIGQYFLKQ
                     KMVEVGGDVRLPPELLTSGAMDLGHSLVKLAGDLANPMLLLFVLIGTATLYAGDYRWE
                     TWRLSSARNTRANLILGKVGVAKMLTLFALVVFFVFDFAGDIAKGLIHGQSFVFHFGG
                     KEAGQMALLALSSYVRVVQVGLLALLAAVVTRSLLATLFVPLVVSVAQFFLMQSMPLF
                     GWEGSEWYAQALIPGLAYDTLKSAIMGGALSGVSSATVWAAAISLILWCGVPLIASIA
                     WFQRQDLSKE"
     gene            complement(3658..4566)
                     /locus_tag="VF633_02630"
     CDS             complement(3658..4566)
                     /locus_tag="VF633_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8469985.1"
                     /translation="MNSVIETRSLTKTYGTVRALDGLSLTIPKGGVYGVLGPNGAGKS
                     TLFRILLGLIRPSEGEAIVMGGPIGDVKASRRMGSMIETPRFPPFMTARQVLVWLSTA
                     HGLTTDPSRVEAWLERVGLTGAADRKVRGFSVGMLQRLGVAVALITEPELIILDEPTS
                     GMDPPGIQEMRALIRSLSDHDGITVVLASHQLLEVQRVCDRVAIMNKGKLVREGTVSE
                     LTTGGERLRLSVKPAERVLEVLGARGSMEGTAVLAAIPRAEGPALLKALIETGIDIDE
                     ARWVGADLESVFMSETGSIQTPESIL"
     gene            4665..5894
                     /locus_tag="VF633_02635"
     CDS             4665..5894
                     /locus_tag="VF633_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX8469986.1"
                     /translation="MNARLPLLMAALSAVLAGCSTPLTTAPTPIVQAAGAATARPLLI
                     LISIDGFRYDYLSRGVTPTLAALAADGAIGPMRPSFPSLTFPNHYTLVTGLHPDHHGI
                     VGNSFVDPKLGKFTMANTEPGWWDQAEPIWNTAEKAGLTTATMFWPGSETTVNGVHPT
                     YHRAFDQALTGEQRVDQLLTWMDATPQPDFATLYFDTVDTAGHRNGPDALATTQAAAA
                     VDASVARLIKGLKARGLYERTVLVIVSDHGMAATSPDRVIVLDDLITPEAVTITYAGP
                     VVYLDPVPGHEAEVEAALVKRHDHGVCWDKAHIPARFVLGSNPRVPAIVCSADIGWVF
                     GTRARPMRQAGGAHGYDNQALEMAALFIAHGPGIAHGRQLQNLDSVDVQPLLGRLLGI
                     TVPAGDGHLTDSAAALQ"
     gene            5957..6847
                     /locus_tag="VF633_02640"
     CDS             5957..6847
                     /locus_tag="VF633_02640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX8469987.1"
                     /translation="MSFESWGVPVALGDRRVLHPGMLRWLRALGWMVLVGFLCVLSFG
                     VPYSTLDHFLPDSGPIDVASNAVCAVIALGVYTALVWFGEARAPSELALKAALPGLFW
                     GLLIGALMFASVMGIMAGFGLYDIVWSGPAPAWKAIGGSIESGVLEELLLRAVILRLV
                     WRAFGPWVAFAASAILFGAGHIGNPNATVFAAVCIAVEAGIMLAAFYALTGRLWVSIG
                     VHAAWNFTQGYLFGAAVSGTDFGPSIAHSTARPGFADWLTGGAFGPEASLPGLFVGTL
                     VGVVVLWMAWKAGRFAKPIT"
     gene            6978..8396
                     /locus_tag="VF633_02645"
     CDS             6978..8396
                     /locus_tag="VF633_02645"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX8469988.1"
                     /translation="MIASSEFASKAAMKSSSIALAAVLFLGACGNANQTKAQDGVFAE
                     PNSRAVPTDAVTMKSSFAPVVRQAAPAVVNISARSVQQVDPFFQMFRGMPAQRMAESA
                     GSGVIVRSDGIVVTNNHVIEGAQQIRVVLNDRREFPAKVLLADERADIAILQLEGVTE
                     QLPVLPIDDREQQEVGDLVLAIGNPFGVGQTVTNGIISALNRTETGISDSGSFIQTDA
                     AINPGNSGGPLIDMDGDVIGINTAIFSRSGGSSGVGFAVPASMVKRVIDSAIGGATSV
                     VRPWLGVKGEGVSADIARSLGMTRPQGLVVTQVYSSGPGDRAGIKEGDVITTVGGQEI
                     NDQGGLNFRVGTHNPNETVAVGLLRDGRPLTVTARIAALPGEADRDQGTVLARGPLAG
                     LKVATLTPAFADSLGADPFTRGIIITGAEGRASNYAFRRGDLIRAVNGQPVNSIADVQ
                     AINGPIQLTIERNGRRITGVIR"
     gene            8453..9766
                     /locus_tag="VF633_02650"
     CDS             8453..9766
                     /locus_tag="VF633_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX8469989.1"
                     /translation="MSDLFEASGVLPPDAPLADRLRPRTLDEVVGQDHLLGPTGPIRR
                     MIEAGRLGSMILWGPPGTGKTTIARLLAKAAGYEFQQISAVFSGVADLKKAFEQARMR
                     RLAGQSTLLFVDEIHRFNRAQQDGFLPFVEEGIVTLVGATTENPSFELNGALLSRSQV
                     FVLKRLDDDALDQLLLRAEAHEGKALPLTPEARHALLALADGDGRYLLTMTEVLFALP
                     EDLMLDVQGLAGTLQRRAPAYDKSREEHYNLISALHKSVRGSDPDAALYWLARMLNGG
                     EDPLYLARRIVRMAVEDIGEADPLSILVANAAKDTYDFLGSPEGELALAQAVVHLATA
                     PKSVGVYEAFKAAKKAAAETGSLMPPAHIRNAPTKLMKSLGYGKGYQYDPDTEEGFSG
                     ANFFPDEMERRTFYKPKGEGHEEKIKGRLERWAVMRARMQAEAEE"
     gene            9994..10728
                     /locus_tag="VF633_02655"
     CDS             9994..10728
                     /locus_tag="VF633_02655"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8469990.1"
                     /translation="MTLRQPVPLSPEEIAAVRSWVIHEDAHVIAFAKPAGLSSQGGRI
                     QAHTLDDLLWAFARSNGKRPELVHRLDRDTSGVILAAKTKPAAGFLGKAIQARKLTKT
                     YLALLSAAPSPPSGRIDAPLLRQEIGRESYMRVVAEDAPGAQASQSRYRTLASSEDGA
                     LVELEPLTGRMHQLRVHMASIGRPLVGDQRYGGALTAAGRAASRLMLHAASLDFPHPE
                     SGRLTVSAPPPEDFQALATAMGLRPD"
     gene            10781..11272
                     /locus_tag="VF633_02660"
     CDS             10781..11272
                     /locus_tag="VF633_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469991.1"
                     /translation="MKRLTVIAIVASTAALAACASLAPYGPQRGPGGQGFSEQRIESD
                     RYRVSYNGVGAPGPVADRALLRAAQLTTEQGYDWFEITQRYIDGRPDSAGGVRPSLGV
                     GYGSSNYGGWRSSGTSVGLGLNFSGPSPTSTTLEVRLGKGPRPDRSEAYDAREIQRSL
                     RAY"
     gene            complement(11269..12471)
                     /locus_tag="VF633_02665"
     CDS             complement(11269..12471)
                     /locus_tag="VF633_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469992.1"
                     /translation="MSHDEHSHAHAPHSTGGSELPTGVEPIPASQAAPAGEPALDIPH
                     VRTAPPATPAPAPRPPVSDDGILRPIQESDYFTLDPTAIRVISANIDEYFNQPLDMFP
                     FWENEGKPDRFHVLFRRQIEERFPVDVDIRGRIYGYGRRVFGALRLHVATKRLTQGLS
                     IVGFGILALLGPTLFEGWTGSQAGGLGLAALSMLIVVGLFFGLSSVLFVQYRFRLEND
                     SYELSREVVQRTRELQNLFTNARAMSDQAETQFSQDGKGWGERALFLTRLTMWIGARM
                     EYLEKFTQMELWRTRRERYWTGWVGGMSVSAVLVVGLLVIGFWPAPGADEIGYRVLQG
                     LAAVLALGVSWLSFYRWQTPMAAVKDKLGVEGWVRYASLDVDNTVGDQVRRDKERLVE
                     YRALTRGR"
     gene            12689..13084
                     /gene="crcB"
                     /locus_tag="VF633_02670"
     CDS             12689..13084
                     /gene="crcB"
                     /locus_tag="VF633_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX8469993.1"
                     /translation="MTRFLLVAIGGALGSTARYGVGLAAGRLFPGSAWPYGTLTVNIV
                     GGLVMGLVAGWLAARGGAGQESVRVFAAVGVLGGFTTFSAFSLETVQMIERRQIGLAV
                     AYVAVSVVVAVAALFLGLLMARRAFGAAL"
     gene            13156..14133
                     /locus_tag="VF633_02675"
     CDS             13156..14133
                     /locus_tag="VF633_02675"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8469994.1"
                     /translation="MLYVAEAEDGIRLDRWFRRRWPHLSNIQVQKMARGGQIRVDGAR
                     IKPEGRLTAGAAVRVPPIPEPIVRAPGEHMALTPADIAFAKSMVIYEDDLVIALNKPH
                     GLAVQGGTKTTRHVDRLLSAWGEGMDRPRLVHRLDRDTSGVLLLGKGPEAAKRLSGAF
                     ARRQAKKTYWAIVHGMPKPNAGQIDMALKKSGINDYEIMRPADPKENGAEPAETAFAL
                     ISHAGQRASWMALRPFTGRTHQLRAHMKAIGHPILGDPKYGDERSNELSGPLKLQLHA
                     RRIELDHPAGGKLIVEAPISAELRAGFNHFGFDEGEADYDPFEGVKRIR"
     gene            14130..15632
                     /locus_tag="VF633_02680"
     CDS             14130..15632
                     /locus_tag="VF633_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX8469995.1"
                     /translation="MTRAGDLSGPERAGRAQALAVAGDLIGAISLLQAAPADQVAGHR
                     TMLAQLLVAADRHDEALAVHIAHAEARPDVAVRHHNVAARLGDMGRVVEAEAAVHRVF
                     SLGGDAPETWLVLARALQGQDRYDEAEVAFREVLARRPDSLDALVDLSQLIWMRTGDG
                     DKARAPLDAAIDAFPDRADLHIVRARLNKFAGVAPQQIHHDLTSAPIAATGALELAAA
                     HAALSFDLDRALKHARAAIARAPADPAAIQQLVEIHLARDEPAAALALLQPILEQRPT
                     DQQALALRNTAWRLAGDPRALGPDDYPAVIAGYQIDTPQGWDTLEAYLADLAAALSGL
                     HGLTTHPVGQSLRHGTQTSANLRHSDDLVIRAFFTAIDGPVRRHMAMLGQGSDPLRSR
                     NTGDYRIAGCWSVRLHPGGYHAPHFHSKGWLSSACHIVLPSVVDGEGKEGWLAFGAPA
                     FEGVRPLPPEHYEKPSPGRLVLFPSYMWHGTVPFSGTEPRLTIAFDVVPA"
     gene            15629..16357
                     /locus_tag="VF633_02685"
     CDS             15629..16357
                     /locus_tag="VF633_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8469996.1"
                     /translation="MTSRLHPKPLAIFDIDGTLVDSRASIFKAAKEAARDLGLPEPEY
                     DRVRQIVGLSLMDAMRALEPSLAEAELAEFVAAFQASFRRMFDAGHEEPLYDGAMEML
                     WRLKDDGWRLSLATGQNRRGISRNLARPGWAELFSSSHCASDGPGKPDPAMLLAALLA
                     CEGTPETAVMIGDTAHDATMARNAGIAAIGVAWGFHTVEEQRAAHVRHVAADFADLEA
                     SLNRFAAELTFEPTAFEPFAFETV"
     gene            16357..17118
                     /locus_tag="VF633_02690"
     CDS             16357..17118
                     /locus_tag="VF633_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEX8469997.1"
                     /translation="MSHIPPLDPRVAAKKGFRESEERLKRFWKAADVVSDGDGWKVVL
                     DGRAPKTSAHAPFVLPTEAAARLVAEEWNAQGEFIEPGTMPATRLAATAIDRVSQTRE
                     PVAEEIAAYAGSDALCYLAEHPTNLVAEQAKAWEPWRDWAAAELGIVLEPTEGIIHRE
                     QDPAAIARVKTLALEADDFALTALATAVPLLGSAVLGLAVMRGALTGEAAFDLSRLDE
                     DFQERQWGVDADNAERTATRRAEMVLVDRWFRALR"
     gene            17199..17648
                     /locus_tag="VF633_02695"
     CDS             17199..17648
                     /locus_tag="VF633_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8469998.1"
                     /translation="MTDPQQTPAEALEAIRKSRTVVAGAVAHNSKTYDLIYSSIAAVM
                     VAGQVLPFPLNVVASTGGAVAFAVLARKWAQKTGVFVSGVTPKRARWVAFGMGAVFVV
                     LMLAALWAGRTHRLWMGIPLGVVAFLVAGVGSRLWLRVFQAETRDRT"
     gene            17645..17935
                     /locus_tag="VF633_02700"
     CDS             17645..17935
                     /locus_tag="VF633_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8469999.1"
                     /translation="MRAPVFDAIIHAPGRLQICAILSDADTAEFALVRDAVGVSDSVM
                     SKHVKQLEDAGYVAVSKAAAGGRQRTWLKLTGEGRAAFRAHVAELTRLAGIG"
     gene            complement(17932..19083)
                     /locus_tag="VF633_02705"
     CDS             complement(17932..19083)
                     /locus_tag="VF633_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8470000.1"
                     /translation="MSPNYTIHVHDSLAAIGREAWDACAEPTGDPFVAYDFLHACEAS
                     GSAAPRQGWAPRHLSLRDENDDVLGVMPLYLKGNSQGEYVFDHSWADAYERAGGRYYP
                     KLLGAVPFTPATGPRFLNAPGTDAATVRQALIQGALGLTEQTGVSSLHVNFPLEAEWE
                     AMGEQTLLLRQDMQFIWRNEGYQTFDDFLAALSANRRKTIRRERRDAQGQLDIRVLTG
                     AQITEDHWDAFFAFYMDTGSRKWGQPYLTRDFFTRVGATMADRIALVMAFRDDAPIAG
                     ALNFIGRDALYGRQWGTLEDIPFLHFELCYYQAIQFAIERGLSRVEAGAQGQHKIARG
                     YLPSPVYSAHYIADPALRAPVARYLEQERPGIAAEIIAMTDELSPYKTP"
     gene            complement(19080..19538)
                     /locus_tag="VF633_02710"
     CDS             complement(19080..19538)
                     /locus_tag="VF633_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8470001.1"
                     /translation="MTVAARIAELGITLPEPAKPVASYVSFVQSGDLVYISGQLSNDA
                     SGGIKGTVGVDVTPEQALAAARLCAINLIAQISAAVDGDLERVVRIVKLGGFVQAGPG
                     FTAIPAVINGCSDLMVEVFGDAGKHARSAVGVYQLPLGFAVEVDAVVEVR"
     gene            19656..20588
                     /locus_tag="VF633_02715"
     CDS             19656..20588
                     /locus_tag="VF633_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470002.1"
                     /translation="METLSPAQVAAFNAVRDRLSAAERAVVDIASVEAVYPAKASASH
                     WNFDLPCHAADEALGEGATDAQRRALIATWVLELPGRVNEADLPFEVLQFYPFWLDKL
                     AAFLSEAAETGATYDRDHWAKDVRFALVLSVPGARTQTIDLSSPMGPGQVVRNGRDGY
                     GWSQLIAYVKAQGWKKPWLEVHTESRHTDDFSEAGWDQAWASAAAICKRRPELAGMIG
                     SSWFYDPPLETISPRMAYLRLNPLKGGAFIIHQGPGQVHSDRAGATSPTRRALIESGE
                     YVARSWLVAWPKSTLIPWADKRAAAMADAERKAA"
     gene            complement(20585..>21519)
                     /locus_tag="VF633_02720"
     CDS             complement(20585..>21519)
                     /locus_tag="VF633_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8470003.1"
                     /translation="NTVSPPSMPLGEVNRAREMEVFARVVDAGGFSAAARDLRLSPSA
                     VSKLVSRLEARIGARLLNRTTRKLILTPEGAAFHEQARRILADMDEAERAAAGGAVPR
                     GRVRVSATVAFGLHCLVPIVPAFSARYPEVVLDLNLTDAVIDLMDQRADVAIRVGKLS
                     GGTLMARKLGTSRTIALASPAYLARYGTPRDLTELAGHNLISFNFVRQRTDWPFLNPD
                     GSISGFPARGNVTAGDGETARALALADAGIVRLAHYHVAEDIEAGRLVPILEAFDPGD
                     HEDLHAVYVGGRATLPSRVRAFIDYLVETVRLDG"
BASE COUNT         3563 a         7142 c         7175 g         3639 t
ORIGIN      
        1 gcgcaaccgt cccgaggacg ccccgatcgg cctgatcgcc gtcgacgccc tgtacagccc
       61 ggtgaagcgc gtcgcctacc gcgtggagcc gacccgcctc ggccagtcgc tcgacttcga
      121 caagctggtc atggaagtcg agaccaacgg cgccgtctcg ccggtggacg ccgtggccta
      181 cgcctcgcgc atcctgcagg accagcttca gatcttcatc accttcgacg agcccaagaa
      241 ggccgtggaa gcgtcggaag gcaagcccga cctgccgttc aacccagccc tgctgaagaa
      301 ggtggacgag ctggaactgt cggttcgttc ggcgaactgc ctgaagaacg acaacatcgt
      361 ctacatcggc gacctgatcc agaagaccga gggcgaaatg cttcgcaccc cgaacttcgg
      421 ccgcaagtcg ctgaacgaga tcaaggaagt gctgacctcc atgggtctgt cgctcggcat
      481 ggacgtgccg aactggcccc cggaaaacat cgaagacctg gccaagaagt tcgacgacca
      541 gatctagttt taaccctccg cccaaatccc caacggggac tgacggggcg gttataaatg
      601 gtacggcggc aggtcctcga aagggatacc gtcgccggag taggagagac cccgatgcgc
      661 cacggcgccg cccaccgcaa gctcggccgt acgagcgctc accggacagc aatgttcgcc
      721 aacatggctg cctcgctgat caagcatgag cagatcacca cgaccctgcc caaggccaag
      781 gagctccgcc ccttcgtcga aaagctggtc acgctcggca agaagggcga ccttcatgcg
      841 cgtcgtcagg ccatcagcca cgtccgcgac gtgacccagg tcggcaagct gttcgaaacg
      901 attggcccgc gttacgccga acgtagcggt ggctacatcc gcatcatgaa ggccggcttc
      961 cgtcacggcg acaacgctcc gatggccgtc atcgagttcg ttgatcgcga cgtcgacgcc
     1021 aagggcctgg actccggtcc ggtttacgcc gtcgaaggcg acgaagaata ggcttcagcc
     1081 acacagctgg aacaccgaaa gggcggcccg agcgatcggg ccgccctttt gaatttacgc
     1141 agggcgctaa cgctcgcgac gtggtcgctc gctgcttgag cgagagggtg gggcttggcg
     1201 ccgtgtcggg tagcgaatag gcgggtggca tgaccagccc cagtcttgag atcgtttcct
     1261 accgccctga ccacgccgag gcatggcggt cgctgaacac cttctggatc ctcgccggtg
     1321 gctacgccat cgaggccaag gacgagttgg tcctcagcga tcccgagggc gcgatcctgt
     1381 cgaagggcgg agccatcttc atcgctgaac tggacgggca ggcggtcgga tgctgctccc
     1441 tcatggccat ggaggatggg ggctacgagg tcggcaagat ggcggtcgac gcagtcgcgc
     1501 acggccgcgg gctcggtgag agattgcttg aagcttgcga agccaaagca ggctctgcat
     1561 cggcgcatcg cctgtatctg gaaaccaacg ccgcccagac gcacgccatc gccctttacc
     1621 gacgcttcgg tttcgtcgat ctgcccgcgc agccgtcgcc ctatgcgcgc tgcaacgtct
     1681 ggatggagaa gcgcctctag cgtccgaaca tctcgtacag ggctgcaccg cagacggcgg
     1741 cggcacacag gccgtgggcg atccaatccc aaacagttcg gcggggcggg gcggggcgtt
     1801 tgtcgaacag tggcattgat cagtatcagc ccgaattgcg ctgaggtgaa aatcgattaa
     1861 acgggatgtt ctttgtgagc tgaggtcaca tgaagcgtcc tgctccgccg cccttaaatg
     1921 ccttgcgcgc cttcgaggcg ttcgctcgcc acggcggcat gacggctgcg ggcgctgagc
     1981 tgtgcgtcac tcacggtgcg gtcagtcgtc aggtgcggat gctgcaggac gtgctcggcg
     2041 ttgtgctggt gaccggtccg cgtcatcgac ttgctcttac ggacgccggc actgcgctgg
     2101 cgcacagcct gtccggcgcg ttcctcgcta tcgacgacgc cgtcgcgacg gcccgagtgg
     2161 ggccattccg cgagattggc atctcatgtc tcggcacgtt cgggctgaaa tggttgatcc
     2221 cccgactgcc gggcttcctc gacgcccacc cagggatccg cgtgcggatg tcggagtcct
     2281 atgctccggc ggacttcaag cgcgaccaga tcgacggggc gatccgtatc tgcgcaccgg
     2341 agcaggcgcc gcgcggccag gaagtgacgc cgttcatgga gttgcagcag ggtctggtca
     2401 tcgcgccgca cctgatcgcg gacgacgcgt cagtcgccgc catcgcgcgt ttgccccggt
     2461 tgcattcggc gacgttccgc gagagctggt ctgtctggag cgagctgatg ggcatcgatc
     2521 tgccgccagc ctgcgtcgaa cgcgagttcg gccataacca ttcgatggtg gaggcggtgg
     2581 ctgcgggcat gggcgccgcg atcgcgccgt gggccttcgt ggctcccgac atcaggagcg
     2641 ggcgcttggc ggcgccgttc agcttcgtcg cccgtccggg gcaccagttc gtgttcctgc
     2701 gacctgaaaa tcgaggggat cctgctgtgg acgcgtttcg cgactggctg gtggcggagg
     2761 gcgcggccag cccgccgccg ccgtccttgc cgacctattc cttcgacagg tcctgacgct
     2821 ggaaccaggc gatggacgcg atcaacggga ccccgcacca gaggatcagg ctgatcgcag
     2881 cggcccagac ggttgcagag gatacgcctg acaacgcccc acccatgatc gcgctcttca
     2941 acgtgtcata ggccagaccg gggatgaggg cctgcgcgta ccactcgctg ccctcccagc
     3001 cgaacagcgg catcgactgc atcaggaaga actgggccac cgagacgacc agcggcacga
     3061 atagcgtcgc caacagtgac cgtgtcacga ccgccgccag caaggccaac agtccgacct
     3121 gaacgacgcg cacgtagctg ctcagggcca gcagagccat ctgaccggct tcctttccac
     3181 cgaagtggaa cacgaatgac tgtccgtgga tcagcccctt ggcgatgtcc ccggcgaagt
     3241 cgaagacgaa gaaaacgacg agcgcgaaca gggtcagcat ttttgcgacg ccgaccttgc
     3301 caaggatcag gttcgcccgg gtgttccgcg ccgaactcaa acgccaggtc tcccagcggt
     3361 agtcaccggc atagagggtc gccgtcccga tcagcacgaa cagcagcagc atcggattgg
     3421 cgaggtcgcc ggccagcttc accagcgaat ggccgaggtc catcgccccc gacgtcagca
     3481 gttcgggcgg cagtcgaaca tcgccaccga cctcaaccat cttctgtttc aggaagtact
     3541 ggccgataga gccaatgatc agcagcacga cgggcaggaa catcgtgctc cagaacacgg
     3601 ccatgcggtt cttggagaag cgataggcct cggaggaaat ggcgtctgtc agcatgctca
     3661 caggatgctc tccggggtct ggatggaacc ggtttcggac atgaagacgc tttcgagatc
     3721 agcgccgacc cagcgggcct cgtcgatgtc gatgccggtt tcgatcaggg ctttcagcag
     3781 ggcggggccc tccgcgcgcg ggatggcggc gaggacggcc gtgccttcca tcgaaccacg
     3841 ggcgcccagc acctcaagca ccctctcggc cggcttgact gacaggcgca ggcgttcgcc
     3901 gccggtcgtc agctcactga ccgtgccttc gcggaccagc ttgcccttgt tcatgatggc
     3961 cacgcggtca cagacgcgct ggacctcgag caactggtgg ctggcgagga cgacggtgat
     4021 cccgtcgtga tcggaaagcg accggatcag cgcccgcatt tcctgaatac cggggggatc
     4081 catgccgctg gtcggttcgt ccaggatgat gagttcaggc tcggtgatca gggcgacagc
     4141 cacaccgagc cgctgcagca tgcctacgga gaagccgcgc accttgcgat cggcggcgcc
     4201 ggtcagcccg acccgttcca accaggcctc gacccgcgac ggatcggtcg tcaatccgtg
     4261 ggccgtcgat agccagacca gcacctgacg ggcggtcatg aagggcggga accggggtgt
     4321 ctcgatcatc gagcccatgc ggcgcgaggc cttcacgtcg ccaatcggcc cacccatgac
     4381 gatggcctcg ccctcgctcg gccttatcag gcccagcagg atgcggaaca gggtcgactt
     4441 gccggcgccg tttggtccga ggacgccata gacgccgcct ttcgggatcg tcagcgagag
     4501 cccgtcgagg gcgcggaccg tgccataggt cttggtcagg gagcgggttt cgataaccga
     4561 gttcatgcgc aaccttggcc tgtgccgcat cgcgcgacaa gccgtcgtga ccctgttgcg
     4621 gattaagctt aggcaatgtt cgtaacccga gtcggagacg agtgatgaac gcccgcctgc
     4681 ctctgctgat ggccgccctt tccgccgttc tggcaggctg ctcgacacct ctgacgacgg
     4741 cgccgacgcc gatcgtccag gctgccggtg cagcgaccgc gcggcctctg ctgatcctga
     4801 tctccatcga cggcttccgg tatgactacc tgtcgcgcgg cgtgacgccg accctggccg
     4861 cgctggccgc cgacggcgcg atcggcccaa tgcggccgtc cttccccagc ctgaccttcc
     4921 ccaaccacta tacgctagtc accggtctgc atcccgacca ccacggcatc gtcggcaaca
     4981 gcttcgtcga tcctaagctc ggcaagttca cgatggccaa taccgaaccc ggctggtggg
     5041 atcaggccga gcccatctgg aacacggcgg agaaagcagg cctgaccacc gcgacgatgt
     5101 tctggccggg ctccgagacg acagtcaacg gcgtgcaccc gacctatcat cgcgctttcg
     5161 atcaggccct caccggcgaa cagcgcgtgg atcaactcct gacctggatg gatgcgacac
     5221 cgcagcctga tttcgccact ctgtatttcg ataccgtcga caccgccgga catcgcaacg
     5281 gcccggacgc tctcgccacc acccaggcag ccgctgcggt ggacgcctcg gtcgcccgcc
     5341 tgatcaaggg gctgaaggcg agggggctgt atgagcggac ggtgctggtg atcgtgtcgg
     5401 accatggaat ggccgcgacc tcgcctgacc gggtgatcgt gctggacgac ctgatcaccc
     5461 ccgaggccgt caccatcacc tacgccggac cggtcgtcta tctggatccc gtcccgggcc
     5521 atgaggccga ggtcgaggcg gcgctggtca agcgccacga ccacggcgtc tgctgggaca
     5581 aagcgcacat cccggcgcgc tttgtgctcg gttcgaaccc gcgcgttccc gcgatcgtct
     5641 gttccgccga cattggctgg gtgttcggca cccgggcccg tccgatgcgt caggcgggcg
     5701 gcgcccacgg ttatgacaat caggccctcg agatggccgc cctgttcatc gctcacggtc
     5761 cgggtatcgc ccacggtcgt cagctgcaga acctcgacag cgtcgatgtc cagccgctgc
     5821 tggggcggct gctcggtata acggttcccg ccggggacgg ccacctcacc gatagcgccg
     5881 ccgccttgca atagcgtaac ttgccttccc aagctgtgcc gtctctgatg gcggcttgtc
     5941 tgggggagtg ccgacgatga gtttcgaaag ctggggcgtg ccggtcgcgc tgggggatcg
     6001 gcgcgtactg catccgggaa tgctgcgctg gctgcgcgcg ctgggttgga tggtgctggt
     6061 cggtttcctg tgcgtcctct cgttcggcgt gccctattcg acgctggatc atttcctgcc
     6121 ggacagtggc ccgatcgacg tcgcttccaa cgccgtctgc gcggttatcg ccctcggcgt
     6181 ctatacggcg ctagtctggt tcggggaggc gagggcgccg tcggaactgg cactaaaggc
     6241 tgccttgccg ggccttttct ggggcctgct gattggcgcg ctgatgttcg cctcggtcat
     6301 gggaatcatg gccggtttcg gcctttacga catcgtatgg agcggcccgg ctccggcctg
     6361 gaaggccatc ggagggtcga tcgagtcggg cgtgttggag gaactgttgc tccgggccgt
     6421 catactgcgg ctggtctggc gcgccttcgg accttgggtc gctttcgccg cctcagccat
     6481 cctgtttggc gcggggcaca tcggcaaccc gaacgccacg gtgttcgccg ccgtctgtat
     6541 cgcggtcgag gccggcatca tgttggcggc cttctacgcc ctgaccggac ggctgtgggt
     6601 gtctatcggc gtccatgcgg catggaattt cacgcagggc tatctgttcg gcgcggcggt
     6661 ctcaggcacg gattttggcc catccatcgc ccacagcacc gctcgccccg gcttcgctga
     6721 ctggctgacc ggcggcgcct ttggtcctga ggcttcactg cccggcctgt tcgtcggtac
     6781 gctcgtcggg gtcgtcgtac tctggatggc gtggaaggcc ggacggttcg caaagccgat
     6841 cacctgaggc gccggctggc gcagttcgtg acgcgaaagg tcgagcaggc ggaacgaacg
     6901 gccgtcgttt gacgctttga aggctcaagc ttgcccgcca tcatcccgcc gaaatggcgg
     6961 agagaaatgc tgccgacatg atcgcctcct ctgagttcgc ctcgaaagcc gcaatgaaat
     7021 cctcctcgat cgctctcgcc gccgtcctgt tcctcggtgc gtgcggcaac gccaaccaga
     7081 ccaaggctca ggacggggtc ttcgccgaac ccaacagccg ggccgttccg accgacgccg
     7141 tgacgatgaa gtcgagcttc gcgccggtcg tgcgtcaggc ggctccggcg gtcgtcaaca
     7201 tctcggcccg gtcagtccag caggtcgatc ccttcttcca gatgttccga ggcatgccgg
     7261 cccagcggat ggccgagtct gcgggctccg gcgtcatcgt ccgctcggac ggcatcgtcg
     7321 tcaccaacaa ccacgtcatt gagggcgccc aacagatccg cgtcgtcctg aacgaccggc
     7381 gcgagtttcc ggccaaggtc ctgctggccg acgagcgggc cgacatcgcg attcttcagc
     7441 tcgaaggcgt caccgaacaa cttcccgtct tgcccatcga cgatcgcgaa cagcaggagg
     7501 tcggcgacct cgtgctcgcc atcggcaatc catttggcgt gggccagacc gtcaccaacg
     7561 gcatcatctc ggccctcaac cggactgaga ccggcatctc cgattcaggc tcgttcatcc
     7621 agactgatgc ggccatcaac ccgggcaact ccggcggacc gctgatcgac atggacgggg
     7681 acgtgatcgg catcaacacc gcaatcttct cgcgctcagg cggctcgtcc ggcgttggct
     7741 tcgcggttcc ggcctccatg gtcaagcgcg tcatcgatag cgccatcggc ggcgcgacca
     7801 gcgtcgtccg cccgtggctg ggcgtgaagg gcgagggcgt gagcgccgat atcgcccgca
     7861 gcctcgggat gacccggcct cagggtcttg tcgtcactca ggtttattcg tccggcccag
     7921 gggaccgcgc cgggatcaag gaaggtgacg tcatcaccac cgtcggcggt caggaaatca
     7981 acgaccaggg cgggctgaac ttccgcgtcg gcacccacaa tcccaacgaa accgtcgccg
     8041 tgggcctgct gcgcgacggc cgtccgctga ccgtcactgc ccgcatcgcc gcgctgccgg
     8101 gtgaggccga tcgcgatcag ggcacagtgc tggcgcgcgg tccgctggcg ggcctcaagg
     8161 tcgcgacact gacgcctgca ttcgccgaca gcctcggcgc cgaccctttc acgcgcggga
     8221 tcatcatcac cggagccgaa ggacgcgcga gcaactatgc gttccgccgg ggcgatctga
     8281 tccgcgcagt caacggccag cccgtaaact cgatcgccga tgttcaagct atcaacggcc
     8341 cgatccagct gacgatcgag cgcaacggtc gccgtattac cggcgtcatt cgctaggtct
     8401 cgcccccgtt ggagcccttc ccccgtcgag ggcgaagggg gtatggacgc cgatgagcga
     8461 tctcttcgaa gcttccggcg tactgcctcc cgatgcgccg ctcgccgatc gcctgcgccc
     8521 ccgcacgctg gacgaagtcg tggggcagga tcatctgctc ggtccgacgg gtcccattcg
     8581 tcgtatgatc gaagccgggc ggctgggctc gatgatcctg tggggcccgc ccggcacggg
     8641 caagacgacc atcgcgcggc ttctggccaa ggcggcgggg tatgagttcc agcagatcag
     8701 cgctgtcttc tccggcgtgg ccgacctgaa gaaggcgttc gagcaggcgc ggatgcgtcg
     8761 cctcgcgggt cagtcgacgc tgctgttcgt cgatgaaatc catcgcttca atcgtgcgca
     8821 acaggatggt ttcctgccgt tcgtggagga ggggatcgtc accctcgtcg gggccacgac
     8881 ggagaacccg tcgttcgaac tgaacggcgc tctgctgtcg cgtagccagg tgttcgtgct
     8941 caagcggctc gacgacgacg cactcgatca gctgttgctt cgggccgagg ctcatgaggg
     9001 caaggcgctt ccgctgaccc ccgaagcgcg tcacgctctg ctggctctgg ccgatggtga
     9061 cgggcggtat ctgctgacga tgaccgaggt gctgttcgcg ttgcctgaag acctcatgct
     9121 cgacgttcag gggctggccg gcaccctcca gcgccgcgcg cccgcctacg acaagagcag
     9181 agaagaacac tacaacctca tatccgcgct gcataaatcg gtgcgggggt ccgatccgga
     9241 tgcggcgctg tactggctgg cgcggatgct gaacgggggc gaggatcccc tgtatctggc
     9301 gcgccggatc gtgcggatgg cggtcgagga catcggcgag gcggacccgt tgtcgatcct
     9361 cgtcgccaat gcggccaagg acacctacga cttcctcggc tctccggaag gtgaactggc
     9421 gctggcccag gccgtggtcc atctggccac tgcgcccaag tccgtcggag tctatgaagc
     9481 cttcaaggcg gcgaagaagg cggcggccga aaccggctcg ctgatgcccc cggcgcacat
     9541 ccgcaacgcg ccgaccaagc tgatgaagtc gctcggctac ggcaagggct accagtacga
     9601 tcccgacacc gaagaaggct tttcgggcgc gaacttcttc cccgacgaga tggaacgccg
     9661 caccttctac aagcccaagg gcgagggcca tgaggagaag atcaaaggcc gcctcgaacg
     9721 ctgggctgtg atgcgcgcgc ggatgcaggc agaagcggag gaataaccat gtccgattct
     9781 accctctccc ggtgggagag ggcttgagcg cccgcaggcg cgggcgatca gcttgcgcga
     9841 aagggtgagg ggttgctggc gggatttgcg gagagggctg tccctcaccc ttttggcttg
     9901 cgcatccctg cgctctgcga gcctcaagcc ctctccgccg gcagaggaaa agagctatgt
     9961 gacgcagcgt ctgctatggg ctagggacgt cgggtgactc tccgccagcc tgtaccacta
    10021 agccctgaag agatcgccgc cgttcggtcg tgggtgatcc atgaggacgc ccacgtcatc
    10081 gccttcgcca aaccggcggg cctctccagt cagggcggtc gcattcaggc gcatacgctg
    10141 gacgacctgc tgtgggcctt cgcccgctcg aacggcaagc ggcctgagtt ggtgcaccgg
    10201 ctggatcgtg acacatcagg tgtaatcctc gcagccaaga ccaagcccgc cgccggcttc
    10261 ctcggcaagg ccattcaggc gcgcaagctg accaagacct atctcgccct gctgtcggcg
    10321 gcgccgtctc cgccgtccgg ccgcatcgac gcaccgctgc tgcgtcagga gatcgggcgc
    10381 gagagctata tgcgcgtggt cgccgaagac gccccgggcg cccaggcatc gcagagccgc
    10441 tatcgcacgc tcgcctcgtc ggaggacggc gccctcgttg aactcgaacc gctgactggc
    10501 cggatgcacc agctccgcgt ccacatggcg tcgatcggtc ggccgctcgt cggggaccag
    10561 agatatggcg gcgcgctgac ggctgctggc agggccgcgt cgcggctgat gctgcatgcg
    10621 gcctcgttgg atttccccca tcccgaaagc ggccgcttga ccgtctcggc gccgccgccc
    10681 gaagacttcc aggcattggc cacggcgatg gggttgaggc ccgactaacg ggaacgaacc
    10741 cgcacgcctg acgctgtgtt tccaaacagg atgttacgtc atgaagcgcc taaccgtcat
    10801 tgccatcgtc gcctcgacag ccgccctggc ggcctgcgcc agcctcgcgc cctacggacc
    10861 tcagcgtgga ccgggcggac agggcttttc cgagcaacgg atcgaaagcg accgttaccg
    10921 ggtgagctat aacggcgtcg gcgctccggg cccggtcgcc gaccgtgcct tgctgcgcgc
    10981 cgcccaactg accaccgaac agggctacga ctggttcgag atcacccagc gctacatcga
    11041 cggacgcccc gacagcgcgg gcggcgttcg gcccagcctc ggcgtcggct acggctcgtc
    11101 gaactacggc ggctggcgtt cgtcagggac cagcgtcggt ctcggtctga acttcagcgg
    11161 gccgtccccg acctccacta cgctcgaagt ccggctcggc aaggggccgc gccccgatcg
    11221 ctcggaggct tatgacgctc gcgagataca aaggtcgctc agggcctact agcggccccg
    11281 ggtaagcgcc cggtactcga ccagccgttc cttgtcgcgg cggacctgat cgccgacggt
    11341 gttgtcgacg tcgagggagg cgtagcgcac ccagccctcg accccgagct tgtccttcac
    11401 cgccgccatc ggcgtctgcc agcggtagaa ggacagccag gacacgccca gcgccagcac
    11461 agccgcaagg ccttgcagca cccggtagcc gatctcgtcg gcgccgggcg ccggccagaa
    11521 gccgatgacc agcagcccga cgaccagcac ggcgcttacc gacatgcctc caacccagcc
    11581 cgtccagtag cgttcgcgac gggtccgcca gagctccatc tgggtgaatt tctcaaggta
    11641 ttccatccgc gcgccgatcc acatcgtcag ccgcgtcagg aacagggcgc gctcgcccca
    11701 gcccttgccg tcctgactga attgggtctc ggcctgatcg gacatggcgc gggcgttcgt
    11761 gaacaggttc tgtaactcgc gggtgcgctg aaccacttct cgcgacaact cgtaggaatc
    11821 gttctccagt cggaaccggt actgaacgaa cagcacgctc gacagaccga agaacagccc
    11881 gaccacgatc aacatcgaca gggcggcgag accgagcccg cccgcctgac tgccggtcca
    11941 gccctcgaac agggtcgggc cgagtagcgc cagaatgccg aagccgacga tcgacaaccc
    12001 ctgcgtcagc cgcttggtcg cgacgtggag ccgaagggcg ccgaacaccc gccggccata
    12061 gccgtagatc cggccccgga tatcgacatc gaccgggaac cgctcctcga tctggcgacg
    12121 gaacaggacg tggaagcggt ccggcttgcc ctcgttctcc cagaacggga acatatccag
    12181 cggctggttg aaatactcgt cgatgttggc tgagatgacc cggatcgcgg taggatcgag
    12241 cgtgaaatag tcgctctcct gaatgggccg caggatgccg tcatcggaca ccggcggtcg
    12301 cggcgcaggg gccggcgttg cgggcggcgc cgtgcggaca tgtgggatgt cgagtgccgg
    12361 ctctccggcg ggcgcggcct gagacgccgg gatcggctcc accccggtag gcagttcgga
    12421 gccgccggtc gaatgggggg cgtgcgcgtg ggagtgttcg tcgtgcgaca tgatgacggc
    12481 ttggccttcg gcgaatgacc gggaactctg tcagacaatg cggtctggtc cgaccacgtt
    12541 ctctcacgac gcgaagctga gccgttgttc gggtatcgcc gttaacccta tgccgccgca
    12601 agtgacgttt cgttcagacg gcgcctgggc cctgcgcagg cgtgtcccgg cttcgcattg
    12661 gcgcacatcc gggtataagc gccggaacat gacacgcttc cttctcgtcg ccatcggcgg
    12721 agcgctcggc tccacggcgc gctacggcgt cgggcttgcg gctggacgtt tgtttcccgg
    12781 ctcggcctgg ccctacggca ccctgaccgt caacatcgtc ggcggccttg tcatggggct
    12841 ggtcgcgggc tggttggctg cgcgtggtgg cgccggtcag gagagcgttc gcgtcttcgc
    12901 cgctgtcggc gtgctcggcg ggttcaccac cttctcggcc ttttcgctgg agacggtcca
    12961 gatgatcgag cggcgccaga tcggtctggc cgttgcctat gtcgcggtgt cggtggttgt
    13021 cgccgtcgcc gctctcttcc ttggactatt gatggcgcga cgagcgtttg gagcggcatt
    13081 gtgagttacg accccagaga agacgccccg gaaacccccg cgccaaaggg gccggtcggc
    13141 gtcgccgagg cccagatcct ctatgtcgcc gaggccgagg acggcatccg gctggaccgc
    13201 tggttccgcc ggcgctggcc tcacctgtcc aacatccagg tgcagaagat ggcgcgtggc
    13261 ggccagatcc gtgtcgacgg cgcccgcatc aagcccgagg ggcgcctgac cgccggcgcc
    13321 gccgtgcgcg tgccgccgat ccccgaaccc atcgtccgcg cgccgggcga gcatatggcg
    13381 ctcacgcccg ccgacatcgc ctttgcgaag tctatggtga tctatgagga cgatctggtc
    13441 atcgccctga acaagccgca cggtctggcg gtgcagggcg ggacgaagac gacgcgccac
    13501 gtggaccggc tgctgtcggc ctggggcgag ggcatggatc gcccgcgtct ggtccaccgc
    13561 ctcgaccgcg acacctccgg tgtcctgctg ctcggcaaag gccccgaggc cgccaagcgc
    13621 ctgtccggag ccttcgcccg gcgtcaggcc aagaagacct actgggccat cgtccacggc
    13681 atgcccaagc ctaatgcggg ccagatcgac atggcgctca agaagtcggg catcaacgac
    13741 tatgagatca tgcgtcccgc cgatcccaag gagaacggcg ccgaaccggc cgaaacggct
    13801 ttcgccctga tcagccatgc gggtcaacgc gcgtcgtgga tggcgctccg ccccttcact
    13861 ggccgaactc accagctgcg cgcccatatg aaggccatcg gtcacccgat cctcggcgac
    13921 cccaaatatg gcgatgagcg ctcgaacgaa ctgtcgggtc cgctgaagct gcagctgcat
    13981 gcccgccgta tcgagctgga ccatccagcg ggcggcaagc tcatcgtcga ggccccgatc
    14041 agcgccgagc tccgcgccgg cttcaaccac tttggtttcg acgagggcga ggccgactac
    14101 gatcccttcg aaggggtcaa gcgcatccga tgacgagggc gggcgacctg tcggggccgg
    14161 aaagggcagg ccgtgcgcag gcgctcgctg ttgccggcga cctgatcgga gccatcagtc
    14221 tgctgcaagc cgcgcctgcc gatcaggttg cgggccatcg caccatgctg gcccagttgc
    14281 tagtcgcggc tgaccgccat gacgaggcgc tggctgtcca catagcccac gccgaggccc
    14341 ggccagacgt cgcggttcgg catcacaatg tcgcggcgcg gctcggtgac atgggccggg
    14401 tggtagaggc cgaggctgct gtgcatcgcg tcttctcact cggcggtgat gcgcccgaga
    14461 cgtggctggt gctggcgcgc gcgcttcagg ggcaggatcg ctacgacgag gcagaggtcg
    14521 ccttccggga ggttctcgcc cgccgtcccg acagcctcga cgctctggtg gacctgtccc
    14581 aactcatctg gatgcgcacc ggcgatggcg acaaggctcg tgcaccgctc gatgccgcca
    14641 tagacgcctt cccggatcgt gccgatcttc acatcgtccg cgcacgcctc aacaagttcg
    14701 cgggagtggc cccccagcag atccatcacg acctgacctc tgcgccgata gccgctacgg
    14761 gcgcgctgga actggcagcc gcccatgccg cgctcagctt tgatctcgat cgcgccctga
    14821 agcatgcccg tgcggcgatc gcgcgcgctc ccgccgatcc ggcagccatc caacaattgg
    14881 tcgagatcca cctggcccgc gacgaacccg cagccgctct cgcgcttctg caaccgatcc
    14941 ttgaacagcg gcctaccgac caacaggcgc tcgctctccg caacaccgca tggcggttgg
    15001 cgggcgatcc gcgtgcgctc gggcctgacg actatcccgc cgtcattgcc ggatatcaga
    15061 tcgacacgcc gcagggatgg gacacgctgg aagcctatct tgcggacctc gccgccgcgc
    15121 tatccggatt gcacggacta accacccatc ctgtcggcca gagcctcagg cacgggaccc
    15181 agaccagcgc caatcttcgc cacagtgacg acctcgtcat ccgcgccttc ttcaccgcca
    15241 ttgacggccc ggtccgacgc cacatggcga tgctggggca ggggagcgat ccgctgcgca
    15301 gccgcaacac cggcgactac cgcatcgccg gttgctggtc ggtgcggctc caccccggcg
    15361 gttatcacgc gcctcatttc cactcaaaag ggtggctctc ctcggcctgc catatcgtcc
    15421 tgccgtcggt ggtggacggc gagggcaagg agggctggct ggcgttcggc gctcccgcat
    15481 tcgagggtgt ccgtcccctt ccaccggaac actacgagaa gcccagcccg ggtcgtctgg
    15541 tgctgttccc ttcctatatg tggcacggaa cggttccctt cagcgggacc gaaccccgac
    15601 tgacgatcgc cttcgacgtg gttcctgcat gacttccaga cttcacccca agcctctcgc
    15661 tatcttcgac atcgacggca cactggtcga cagccgggcc tcgatcttca aggcggctaa
    15721 ggaagcagcg cgcgatctgg gcctacccga gcctgagtat gaccgggtgc gccagatcgt
    15781 cggcctgtcg ctgatggacg ccatgcgagc gttggagccg tcgctggccg aggccgagct
    15841 ggccgagttc gttgcggcgt ttcaggccag cttccggcgc atgttcgacg caggtcacga
    15901 ggagccgctg tacgacggcg cgatggagat gctttggcgg ctgaaggacg acggctggcg
    15961 tttgtcgctg gcgacaggcc agaaccggcg gggcatctcg cgcaacctcg cgcgtccggg
    16021 gtgggcggag ctgttttcgt ccagccattg cgcatccgac ggtccgggga agcccgatcc
    16081 cgccatgctg ttggccgctc tcctcgcctg cgagggcacg cctgaaaccg ccgtgatgat
    16141 cggggacacc gcccatgacg cgaccatggc ccgcaacgcc gggatcgccg ccatcggcgt
    16201 ggcctggggc tttcacacgg tcgaggaaca gcgtgcggcg catgtccgtc acgtcgccgc
    16261 cgacttcgcc gatctggagg cgagcctgaa ccggttcgcc gccgaactga catttgagcc
    16321 caccgccttc gagcccttcg ctttcgagac tgtctgatga gccatatccc gccgctggac
    16381 ccccgcgtcg ccgccaagaa gggcttccgc gagtccgagg aacggctgaa acgcttctgg
    16441 aaggcggccg atgtcgtctc tgatggcgat ggctggaagg tcgtcctcga cggccgtgcg
    16501 cccaagacct cggcccatgc gccgttcgtc ctgccgaccg aggcagccgc gcgactggtg
    16561 gcggaggaat ggaacgctca gggcgagttc atcgagcccg ggaccatgcc ggcgacgcgc
    16621 ctcgccgcca ccgccatcga ccgggtcagc cagacgcgcg agccggtcgc cgaggagatc
    16681 gccgcctacg ccggttcgga tgccctctgc tatctggccg aacacccgac caacctcgtc
    16741 gccgaacagg ccaaggcatg ggagccgtgg cgcgactggg ctgcggctga actgggcatc
    16801 gtgctggagc cgaccgaggg catcatccat cgcgaacagg acccggcggc catcgcccgg
    16861 gtaaagacgc tggcgctgga agccgacgac ttcgccctga cggcgttggc gacggccgtg
    16921 ccgttgctgg ggtcggcggt actggggctg gcggtgatgc gcggcgcact gaccggagaa
    16981 gcggctttcg atctcagccg cctcgacgaa gactttcagg aacgccagtg gggcgtcgac
    17041 gccgacaacg ccgaacgcac cgccacccgt cgggcggaga tggtgctggt tgatcggtgg
    17101 ttcagggctc tccgataaac tttccaatct ggaaagttat ctcttgacga tgtggaaagt
    17161 cagcgtcatt ttccagtatg gaaagtgagg gctcaacaat gaccgatcca caacagactc
    17221 ccgccgaggc gctggaagcc atccgcaagt cccgtaccgt cgtcgcgggt gcggtggccc
    17281 ataactcgaa gacctacgac ctgatctact cctcgatcgc ggcggtgatg gtcgccggac
    17341 aggtgctgcc ctttccgctg aacgtggtgg cttcgaccgg cggtgcggtg gcgttcgcgg
    17401 ttctggcccg caaatgggcg cagaagaccg gcgtgttcgt cagcggcgtg acacccaagc
    17461 gtgcgcgatg ggtggcgttc ggcatgggcg cggtgttcgt ggttctgatg ctcgcggcgc
    17521 tgtgggcggg tcggacgcac cgcttgtgga tgggcatccc gctgggggtc gtggccttcc
    17581 tggtggcggg cgtcgggtcg cggctgtggt tgcgggtgtt ccaggccgag acgcgggacc
    17641 gcacgtgagg gcgcccgtct tcgacgccat catccatgcg ccgggccgcc tccagatctg
    17701 cgccatcctg tccgacgccg acaccgccga gttcgcattg gttcgtgacg ccgtcggggt
    17761 ctccgactcg gtcatgtcca agcatgtgaa gcagttggaa gacgcgggct acgtcgcggt
    17821 ttcgaaggcc gcagcgggcg ggcgtcagcg cacctggctc aagctcaccg gggagggccg
    17881 cgccgcgttc cgggcgcacg tcgccgaact gacgaggctg gcggggatcg gctagggcgt
    17941 cttgtacggc gacagttcat ccgtcatggc gatgatctcg gcggcgatgc cggggcgttc
    18001 ctgttccaga taccgggcca ctggcgccct cagcgcgggg tcggcgatgt agtgggcgga
    18061 gtagacgggg gagggcaggt agccgcgtgc gatcttgtgc tggccctgag cgccggcctc
    18121 gacgcgggac aggccgcgtt cgatggcgaa ctggatcgcc tgatagtagc agagctcgaa
    18181 gtggaggaag gggatgtcct ccagcgtgcc ccactggcgg ccgtagaggg cgtcgcggcc
    18241 gatgaagttg agcgctccgg cgatcggggc gtcgtcccgg aacgccatga ccagcgcgat
    18301 ccggtcggcc atcgtggccc cgacccgggt gaagaagtcg cgggtcagat agggctggcc
    18361 ccacttgcgc gagcccgtgt ccatgtagaa ggcgaagaag gcgtcccagt gatcttcggt
    18421 gatctgggcg cccgtcagga cgcggatatc gagctggcct tgcgcatcgc ggcgctcgcg
    18481 gcggatggtc ttgcggcggt tggccgacag ggcagcgagg aagtcgtcga aggtctggta
    18541 gccctcgttc ctccagatga actgcatgtc ttgccgcagc agcagcgtct gctcgcccat
    18601 cgcctcccac tcggcctcaa gcgggaagtt gacgtgcagg gacgagacgc cggtctgctc
    18661 ggtcaggccg agcgcaccct ggatcagggc ttgccggacg gtggcggcgt cagtgccggg
    18721 cgcgttgagg aagcgcgggc cggtcgccgg ggtgaagggc acggcgccta gcagcttggg
    18781 atagtagcgt ccgccggcgc gctcataggc gtcggcccag ctgtggtcga agacgtattc
    18841 gccctggctg ttgcccttca gatagagggg catgacgccg aggacgtcgt cgttctcgtc
    18901 gcgcagggac aggtggcgcg gcgcccagcc ctgacgcggc gccgcactgc ccgaggcctc
    18961 gcaggcgtgc aggaagtcgt aagcgacgaa cggatcgccg gtcggctcgg cacaggcgtc
    19021 ccaagcctcg cgaccgatgg ccgcgaggct gtcatgaacg tggatcgtat agtttgggct
    19081 cagcggactt ccacgacggc gtcgacttcg acggcgaagc cgaggggcaa ttgatagacg
    19141 ccgacggccg agcgggcgtg cttgccggcg tcaccgaaga cttcgaccat caggtccgag
    19201 caaccgttga tgacggcggg gatggcggtg aagcccggtc cggcctgaac gaagccgccc
    19261 agcttgacga tgcgcacgac gcgctcgagg tcgccatcga cggcggcgct gatctgggcg
    19321 atcaggttga tggcgcagag gcgggcggct gcgagggcct gttccggggt gacgtcgacg
    19381 ccgacggtgc ccttgatccc gcccgaggcg tcgttcgaga gctggcccga gatgtagacg
    19441 aggtcgccac tctggacgaa ggagacatag ctggcgacgg gcttggcggg ctcgggcagg
    19501 gtgatgccga gttcggcgat acgggcggcg acggtcatgc tgtgggctcc tttggggatg
    19561 tcgcggggtt tagcgcgccg cgccctcgcc gtcatccgtc aagcatgtgg ggaggcctta
    19621 aaggttcgcc aaccgccgtg tgtcagggtg cgtggatgga aacgctctct cccgcccaag
    19681 tcgcggcctt caatgccgtt cgtgaccgcc tcagcgccgc agagcgcgcg gtggtcgaca
    19741 tcgcttcggt ggaggccgtc tacccggcca aggcttcggc cagccactgg aacttcgacc
    19801 tgccttgcca cgccgccgat gaagcgcttg gcgagggcgc gacggatgcg caacgccgcg
    19861 cgctgatcgc gacctgggtg ctggagctgc cggggcgggt caacgaggcc gacctgccgt
    19921 tcgaggtgct tcagttctat ccgttctggc tcgacaagct ggccgccttc ctgagcgagg
    19981 ctgccgagac gggcgccacc tacgaccgcg atcactgggc caaggacgtg cgcttcgcgc
    20041 tggttctgag cgtgccggga gcgaggaccc agaccatcga cctgtcgtcg ccgatggggc
    20101 cgggtcaggt cgtccgcaac ggtcgcgacg gctatggctg gagccagttg atcgcctatg
    20161 tgaaggccca aggctggaag aagccctggc tcgaggtcca caccgagagc cgtcacaccg
    20221 acgacttctc ggaagccggc tgggatcagg cctgggcttc cgctgcggcg atctgcaagc
    20281 gccgtccgga gttggccggc atgatcgggt cgagctggtt ctatgatccg ccgctggaga
    20341 cgatcagccc acgcatggcg tatctgcggc tcaacccgct gaagggcggc gccttcatca
    20401 tccatcaggg accgggacag gtgcactcgg atcgggccgg agcgacatcg ccgacacggc
    20461 gcgccctgat cgagtccggt gagtatgtcg cccgctcgtg gctggtcgcc tggcccaagt
    20521 cgacgctgat cccgtgggcc gataagcgcg ccgctgcgat ggcggacgcc gagcgcaagg
    20581 ctgcctaacc gtccagcctg accgtctcga ccagatagtc gatgaaggcg cggacgcggc
    20641 tcgggagggt ggcgcgaccg ccgacataga cggcgtgcag gtcctcatgg tcgccgggat
    20701 cgaaggcttc gaggatcggg accagccgtc ccgcctcaat gtcctcggcg acatgatagt
    20761 gggcgagccg gactatgcct gcatccgcca aagccagcgc ccgggccgtc tcgccgtcac
    20821 cggcggtgac gttgccgcgc gccgggaagc cgctaatcga gccgtcggga ttgaggaacg
    20881 gccaatcggt ccgctggcgc acaaagttga agctgatcag gttgtgaccg gcgagttcgg
    20941 tcaggtcgcg cggcgtgccg tagcgggcca gataggcggg cgaggcgagg gcgatggtgc
    21001 ggctggtccc cagcttccgc gccatcaggg tcccgcccga cagcttgccg acccggatgg
    21061 cgacgtcggc gcgctggtcc atcaggtcga tgacggcgtc ggtcaggttg agatcgagga
    21121 ccacctcggg atagcgagca ctgaaggccg ggacgatggg cacgaggcag tgcagaccga
    21181 aggcgaccgt cgcgctgacc cgcacccgac cgcgcggcac cgcgccgccc gccgcagcac
    21241 gctcggcctc atccatgtcg gcaaggatgc gccgcgcctg ttcgtgaaac gccgccccct
    21301 ccggcgtcag gatcagcttc ctggtcgtgc ggttgagcag ccgtgcgcct atgcgggcct
    21361 ccagccgtga caccagtttc gacaccgccg acgggctgag ccgcaggtcg cgcgccgccg
    21421 ccgagaagcc gcctgcatcc acgacgcgag cgaacacctc catctcgcgg gcgcggttga
    21481 cctccccgag aggcatactg ggcggcgaca cggtattca
//
LOCUS       DASVTM010000095         8689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_754895,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000095 DASVTM010000000
VERSION     DASVTM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754895"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VF633_02725"
     CDS             complement(<1..1027)
                     /locus_tag="VF633_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX8470004.1"
                     /translation="MKLDLNPQKIVRRIPVEWLDKYQKGVSGPSGALDDSRKLVRFGL
                     IVLVGFFGVLIVWAALAPLNGAAVASGIVTVSGNRQSLQTLNGGVVEAVLVREGETVK
                     AGQVLIRMNGIAAGGKYRQTQAQRDALRAAEARLLAERDNASEIQVPQELRTRQFEPA
                     VSQALANQRALFDSRKKVFDADRGIYDARVQQARSEVNSPSEQLRYVREQVAGMRTLY
                     RKGFAPKSTLFELERLRVELEAQAVAGGAKVAEAQLLAARAEQERVGEVVEQLRLVQD
                     QLQQVDPDLAVARYNAERNVVRAPLAGAVVDLARVAPGSIIGSGEKVMDILPSGRGLI
                     VEAKIKPE"
     gene            complement(1035..2753)
                     /locus_tag="VF633_02730"
     CDS             complement(1035..2753)
                     /locus_tag="VF633_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX8470005.1"
                     /translation="MPEPTTAPKDEFANALSACLPLFWTAVAFGGGVNLLFLASPLFM
                     LQIYNRVIPSGSISTLVGLAFALIIALVTMACLDAVRSRVLIRAAARLDRLLAPRVFQ
                     AMTDLSLRTGANTRNAQALRDLDTFRSAMAGPAAQLFFDVPWSPLFIILLFLLHPLLG
                     LIGLLGALMLLGLAFLNDALTREDAAGASAAAGRSYGFADSIVRFSDPVHAMGMNDAL
                     QVRWRIDRDNMMGKQSAGSDRSADMSAFIRFSRLLLQSAMLGVGAWLVIEGQMLPASI
                     FASSLLLGRALSPLEQAVVGWRQMAIALNAGRNVQRVLNAAPPQPQRLRVPPKAGGVI
                     LERVVFVPLGAPKPSLLDIDLMIEAGETVGVVGASGAGKSCLARLLVAASLPTRGKAA
                     IAGISTNKWTADALARHVGFLPQNVGLFPGTIRDNISRFLDADDQAVVQAARRARVHD
                     MIMDLPDGYETGIGDGGAGLSGGQRQRIALARALFGSPRLLVLDEPNAHLDAEGEEAL
                     SDSLKALKQTGSTIVIVAHRLNPLAQVDRLLFLKNGRLEMDGPRQTVIDQVPTEVVEH
                     FAQPAA"
     gene            complement(2821..3084)
                     /locus_tag="VF633_02735"
     CDS             complement(2821..3084)
                     /locus_tag="VF633_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470006.1"
                     /translation="MGILSNVLGGDQDSSSNSDSSAAGVLQSATDLGVSYEANSSDMN
                     EDGETTSSHDDGAFGTDLNTGNLLGGASDSSSDSSTDSDGGSF"
     gene            complement(3236..3883)
                     /locus_tag="VF633_02740"
     CDS             complement(3236..3883)
                     /locus_tag="VF633_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470007.1"
                     /translation="MPATTVGDSSGSTLSHVEQFVGLPDAETGLTQFVAAAQGAVAGG
                     QDSLIDPALDQISATSSLVEGALAPALASASYPALTAAAVTGALADGLGQTAADIGGL
                     TAGALAPVSTAIEAAVPGADLVSSIASQTDPDLGAITDIPAVFDEPAGNIPEAGAAPG
                     LLAALFTPHNDTADTSAPVGHEQSPELATDLAPDLDLGGFLRTTDDHVDLGHIGL"
     gene            complement(4465..5199)
                     /locus_tag="VF633_02745"
     CDS             complement(4465..5199)
                     /locus_tag="VF633_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8470008.1"
                     /translation="MREITSGDLLRRLQRLAPLDPVAEDLVAGMVRPGTPHPAGAVLL
                     QQGEAVRGPHLLTSGWAWRTRTLPSGRQQVFAIVLPGDFMGLRWRPNPRALSSTVAVT
                     SVQTCEVAALQTVMGRKGETHRGLWEALTLVSRHDEMRLLDQVVRLGQQSAIERVSSL
                     MLELHSRLSVVGLTDDYSFQMPLTQEHLAVTLGMSLVHVNRTVQLLRKEGLAEIGSGR
                     ATLLEPDRLRSMASFDATAKAYAEAV"
     gene            6070..6780
                     /locus_tag="VF633_02750"
     CDS             6070..6780
                     /locus_tag="VF633_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8470009.1"
                     /translation="MSDTIETSSSCFGNVSLLFPRGELRAFLPDRSQMHGVVLASGHP
                     LTDLLGDYLLSLTQRAGLLSVGEAMLAAGATVALAGAVLAGQPAGPDRQTVCGQPSDL
                     RRIGQYVDAHIGDPKLDAVRIARSLGMSRASLYRACLSSGGVANLIRRRRLTGAALQL
                     ADPLQRERIGEIARRWGFTSDSAFARTFREAFGISPREARRRGSAPVSTNADGSVDAS
                     GFASWMRLLRPTGECSNR"
     gene            7011..7904
                     /locus_tag="VF633_02755"
     CDS             7011..7904
                     /locus_tag="VF633_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470010.1"
                     /translation="MGARLLFLVSLIATGVGTAMPDTAGAQTVALTPEQMSAFNQQVV
                     HARQAQTVVGKSEACVTLYNDGLQRRSSQLETALADNLRLERALDVELIRLNEERGQF
                     SSIALAEQRNLDEKQRDLQSAIAERQEQDRRLQECRRAMTFLAFLCDWGDQAVRDLGW
                     MRNVQAEIGGIEARLESRRNGMLDAERRLAASHTALTEAERQIAASQKAIPQIEAQIV
                     QVKATIADLHVMIQSYNTLLDDFASLLEHADAVTAGSPRAREIIRLSSNIEDLVSRSP
                     AAVSAASAILPEDLKQACSTH"
     gene            7952..>8689
                     /locus_tag="VF633_02760"
     CDS             7952..>8689
                     /locus_tag="VF633_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470011.1"
                     /translation="MAQVLEFESRNAQIAADIRSGVDRIRGVSPLALPGPEAAIVKSY
                     VQKIQGTYDVERARTDTDNAIDLLYIAYNTTPQMEGDVRNTISALMNKVITAQQDSER
                     TMKGAMRVANIIVNSLDDVFFEWLDAKESDSEADLKRFVSTDLLRLATEIKTQALRIR
                     DELLAVASTYDGIIRDNVAATSISEKALSVQLKQKAAVEKEMNDATARREQLESLVVD
                     LKDQVAKFDKMARDFEARASTAEQRAFV"
BASE COUNT         1624 a         2722 c         2785 g         1558 t
ORIGIN      
        1 cttcgggctt gattttggct tcgacgatca atccccggcc gctcggcaaa atatccataa
       61 ccttttcgcc ggagccgatt atggagcccg gtgcgacccg ggcaagatct acgaccgcgc
      121 cggcgagggg agcccgcacc acgttgcgct ctgcgttgta tcgagcaacg gccagatcgg
      181 gatcgacctg ctgcaattga tcctgaacga ggcgaagctg ttcgacgacc tcaccgacgc
      241 gttcctgctc cgccctggcg gcgagcagtt gcgcttccgc gacctttgcc ccgcctgcta
      301 ctgcctgggc ctcaagctcg acccgaagcc gctcaagctc gaacagcgtg ctcttgggcg
      361 cgaaaccttt gcggtagagg gtccgcatgc cggcgacctg ttcgcggacg taccgtagct
      421 gttcggacgg actgttcact tcgctgcgcg cctgctggac acgcgcgtcg tagatgcctc
      481 gatcggcatc gaacaccttc ttgcggctgt cgaaaagagc gcgctgattt gccaaagcct
      541 gggaaacggc cggctcaaac tgacgcgtac gaagttcttg cgggacctgt atttcggagg
      601 cgttgtcgcg ttcggccagc agcctcgctt ccgccgcgcg caaagcatct ctctgggcct
      661 gggtctgtcg atattttccg ccggccgcta ttccgttcat ccggatcagg acttgtccgg
      721 ccttcaccgt ttcgccttcc ctgaccagga cggcctcgac gacgccgccg ttgagggtct
      781 gcaggctttg gcgattgccg gacaccgtca cgatcccgct ggccactgca gctccgttta
      841 acggggcgag cgcggcccaa acgatcaata cgccgaagaa gccgaccagg acgatcaggc
      901 cgaaacggac aagttttcgg gaatcgtcga gcgcgcccga cggccccgag acgcccttct
      961 gatatttatc aagccactcg acggggatac gccggacgat cttctgggga ttaaggtcca
     1021 gtttcatctc ggcctcacgc cgctggctgg gcaaaatgtt cgacgacctc cgtcgggacc
     1081 tgatcgatga cggtctggcg gggtccgtcc atctccagcc gaccgttctt gaggaacagc
     1141 agccggtcga cctgtgccag tgggttcagt cgatgggcca cgatgacgat cgtcgatccc
     1201 gtctgcttga gagccttgag gctgtcgctc aacgcttcct cgccttcggc gtcgaggtgg
     1261 gcgttcggtt cgtcgagaac gagcaggcga ggcgagccga agagggcacg ggcgagcgcg
     1321 atccgttgcc gttggccgcc tgaaagcccg gctcccccgt ctccgatccc ggtttcgtaa
     1381 ccgtccggga gatccatgat catgtcatgg acccgcgccc gtctggccgc ctgcacgacg
     1441 gcctgatcgt cggcgtcgag gaagcgactg atattgtcgc ggatcgtgcc cgggaacagg
     1501 ccgacattct ggggcaggaa gcccacgtgg cgtgccaggg cgtccgccgt ccacttgttg
     1561 gtcgagatcc cggcgatagc cgccttgccc cgggtcggaa gcgaggccgc aaccaggagc
     1621 cgcgccagac agctcttgcc ggcgccactc gcgccgacca cacctacggt ttcaccagcc
     1681 tcgatcatca ggtctatgtc cagcagggac ggtttgggtg cgcctagcgg tacgaacaca
     1741 acgcgttcga ggattacgcc gcccgccttg ggtgggaccc gcagtcgctg gggctggggc
     1801 ggagcggcgt tcaggacccg ctggacgttt cggcccgcgt tcagggcgat cgccatctgg
     1861 cgccagccta ccacggcctg ctcaagaggc gacaaggcac ggcccagcag gagacttgag
     1921 gcgaagatac tggccggcag catctgcccc tcgattacga gccaggcgcc cacccccagc
     1981 atggcgcttt gcaagagcag gcgactgaag cgaatgaaag ccgacatgtc cgcgctgcgg
     2041 tcggagcccg cactctgctt tcccatcata ttgtcgcggt cgatgcgcca gcgcacctgc
     2101 aaggcgtcgt tcatgcccat ggcgtgcacc ggatcggaga agcggacgat gctgtcggcg
     2161 aacccatagc tgcgcccggc ggcagcgctc gctccagccg catcttcacg ggtcagggcg
     2221 tcgttgagaa acgcgagtcc cagcagcatc aaagctccca agaggccgat caggcccagc
     2281 agcggatgca gcagaaacag aagaatgatg aacaggggtg accagggtac gtcgaaaaag
     2341 agctgcgccg ccggaccagc catggcggag cggaacgtgt cgaggtcgcg caaggcctgc
     2401 gcgttgcggg tattggcccc ggtccgcagg gacagatccg tcatggcctg gaagacccgg
     2461 ggagcaagga gccggtcaag ccgcgccgca gcgcggatca gaactcgaga gcgcacagcg
     2521 tccagacagg ccatggtcac caaggcaatg atcagtgcaa acgcaaggcc cacgagggtg
     2581 ctgatactgc cgctcgggat aacccgatta tagatttgca acatgaacag gggggaggcc
     2641 aggaacagca ggtttacccc gcctccaaat gcgaccgcag tccagaacag cggaaggcag
     2701 gcgctcaggg cattggcaaa ctcgtccttg ggggccgtgg tcggctcagg cacggttcct
     2761 cccgtcagaa cagaaagcga tgggaggcgg cagtgccgcc tccctcacaa tggcgtcgga
     2821 tcagaatgaa ccgccgtcgg aatccgtcga gctatcggac gaactgtccg aggcgccgcc
     2881 gagcaggttg ccggtgttca gatcggtgcc gaaggcgccg tcgtcgtgcg agcttgtggt
     2941 ctcgccgtct tcgttcatgt ccgagctgtt agcttcgtag ctaacgccta gatcggtcgc
     3001 ggattgcagc acgccggcgg cgctggaatc gctgttgctg ctggagtctt ggtcgccgcc
     3061 aagaacattg ctcagaatac ccatagtaca tctcctgttg ttgtttgcga tgcgccgggt
     3121 tttgacagat gtatgtaatg tccgggctcg ccaatattaa acggagatgc agaacgtaag
     3181 tttcagctaa gccattatat ttaaatgact atatcatttg ttcacgtcaa atggtctaga
     3241 gcccgatatg gccgaggtcg acgtgatcat ccgtcgtcct cagaaatccg cccaggtcta
     3301 gatcgggcgc cagatcggtc gccagctcgg gcgactgctc gtggccaaca ggcgcactcg
     3361 tgtccgcagt gtcgttgtgg ggcgtgaaca gggccgccaa aagccccggc gccgcgcctg
     3421 cttcagggat gtttcctgca ggttcatcga agactgcggg aatatccgta atcgccccca
     3481 ggtccgggtc agtctgagac gcgatggacg agacaaggtc agcgcccgga accgcagcct
     3541 cgatggcggt cgagacagga gcgagtgcgc cagctgtcaa tccgccgatg tcggcggcgg
     3601 tttggccgag cccgtcagcg agcgcgcctg ttaccgctgc tgcggtcagg gccggatacg
     3661 atgctgacgc cagagccggt gcgagagccc cctcaacgag agaagacgtg gccgagatct
     3721 gatcgagcgc aggatcaatc agggagtcct gcccaccggc gacggcccct tgagcggcag
     3781 cgacgaactg ggtcaggccc gtttcggcgt caggcagccc gacgaattgc tctacgtgcg
     3841 aaagtgtcga tcccgagctg tccccgaccg tcgtggcggg catcgaaccc ccaaggctgg
     3901 tcgcctgtga caagccgacg gagatttcac tcgatcgaac atgatcaccg ccagcgtcgc
     3961 tccagcccat ttccccggcg atcgtcagct gtaagtcttg atcaagatcc gcccgggtag
     4021 acgcctcctc cgtcgagccc cggtcagccg gctgcggccc ggtgggcacg gttccgggat
     4081 acgtccccgc cgggagacct gccggcggtg cggctgggcc tgcggctgcc ggggtgggtt
     4141 ctccctgacc agtggatgtg cccaggtgag gggctgtccc cgatccgacg ccatcggctg
     4201 gatccggttg gggctgggag cccatgggat ccgttgaagg agcgaccggc tcctcggcgt
     4261 cgtgtcgcgc ggcctgttgc tcccagtgct cgtcgggctg atcactgacc ggggcgagat
     4321 cggcctgctg ggcgcccgag ggcgcggttc gggcgtcgga acggtgaagc ggatcgccag
     4381 atgtgtcgga catggaggtg ccttgtggaa atgaggcatc tggcgagacg ataatgcgcg
     4441 gcgcccgctt ttgatccctg ccgcctagac agcctcggcg taggccttgg ccgtggcgtc
     4501 gaaactcgcc atcgaacgca accggtcggg ttcgagaagc gtggcccgac cgcttccgat
     4561 ttcggcaagc ccttccttgc ggagcagttg gaccgttctg ttcacatgga ccagactcat
     4621 ccccaaggtc acagcaagat gttcctgggt caggggcatc tggaaactgt agtcgtccgt
     4681 caggcccacc accgacaacc ggctgtggag ttcgagcatg agactggaga cgcgctcgat
     4741 cgccgattgc tggcctaaac gaaccacctg atcgagaagg cgcatctcat cgtggcggga
     4801 gaccagggtc agcgcctccc acaggcctct gtgagtttcg cctttgcgac ccatgacggt
     4861 ctgcaatgcc gctacctcac aggtctggac cgaagtcaca gctacggtcg aagacaaagc
     4921 cctcggattg ggacgccagc gcagacccat gaagtctccg ggaaggacga tggcgaagac
     4981 ctgctggcga ccgcttggca gggtacgggt tcgccacgcc catccggagg tgaggagatg
     5041 gggaccgcgg acggcctcac cctgttgcaa caggacagcc ccggcgggat gaggcgtccc
     5101 cggccggacc atacctgcga caagatcctc ggcgaccgga tcgagcggcg ccaacctctg
     5161 aagacgccgc aacaaatcgc cggatgtgat ttcgcgcaag cgtctctctc cgagttaccg
     5221 ggcgatcgct gaatgcgtcc cggacgatag agcctccggc gccgccccgt tccctatcgg
     5281 gacgagatgc tcgcactgac ggctgtcaga tcagctcctg acctgatccg gagcacctaa
     5341 actcctaaga tcgctggcaa acccttgcgc gacgccgttg gttcctgagt cgtgaaacca
     5401 acatttttca aggcatgccg cgaagaggcc aacccttttc gtcgcgaagt cagcgccggg
     5461 aacgacggct ttcacgttcg cccggctgat caaggcggtc agatgctagc cgctggcggg
     5521 attacaggtg tccccgccgg ggtgatctga ttgcgaacgg ttggatcggc ccctcaaccg
     5581 tattcgcgtt aaagttgatt catgcgcctc aggcgtgtat tgagaagcgg tgatggagtc
     5641 gccgccagtc ccgctccgtt tcaatgattt tgccacagta ccggttgatc gttgcggtcg
     5701 ttcctcacgc gtgagctcgg tctgtgggcc aaaggatgcg acctccgtac cctcattccg
     5761 gatcaacggt tcagaccttg ggccggaggc ggctctgaag cagttccgga tcgccgttca
     5821 ggatgtcttt tcggtcgaac tgacaagccg cgaagacgcc gtcgcactgg acgctgtgag
     5881 ttggcacctc ggaaacatga tgctcggtgt attccgggga cccccctgat cttcaggcgg
     5941 tcaccggcgc tcgtggcgac cagcggactc gatcacattc tggttcagct atattttgaa
     6001 ggcggcttcg tcggctccgc gggtacgagt tcggtctgcg tcgctgcggg agacatttgc
     6061 gtctttgact tgagcgacac tatcgagaca tccagcagct gtttcggcaa cgtcagcctc
     6121 ctgtttcccc gtggcgagtt aagggccttt ctgccggacc gaagccagat gcacggggtc
     6181 gtgctcgcct ccggccatcc gctgaccgac ctccttggag actacctctt gtccctgaca
     6241 cagcgggccg gcttgttgag cgtcggcgag gccatgctgg cggcgggcgc gaccgtcgct
     6301 cttgcgggcg ccgtgctggc cggccagcca gcggggcccg accgccaaac agtctgtggt
     6361 cagccctccg atctgcgcag gatcggccaa tatgtagacg cgcatattgg tgatcccaaa
     6421 ctcgatgcgg tcaggatcgc gaggagcctg gggatgtcgc gcgccagcct gtaccgcgcg
     6481 tgcctgtctt caggaggtgt tgcgaacctg atccgacggc ggaggttgac cggagccgcg
     6541 ctgcagttgg ccgatccgct gcaacgcgag cggatcggcg agatcgcccg tcggtggggt
     6601 ttcaccagcg actccgcctt cgcccgcaca tttcgggagg cgtttggaat atcccctcgc
     6661 gaagcgcgtc ggcgcggatc cgcccccgtg tccaccaatg ctgacgggtc ggttgacgct
     6721 tcagggttcg ccagctggat gcggctgctg cgcccgacag gcgagtgttc caatcggtag
     6781 gcgtgactgg cgcggccagc cctggaaggc aggcttgcca gaacggcgcg gcggggaatt
     6841 ggtccgcacg tctcagatac ttgacgcttc atcgcgtttg ttgggcaggc gctcgccttc
     6901 ccggatcttc ctgataccga cgacaataga ggggcggaaa cccttgggca ggagccccct
     6961 caccagcacc cggtttcggg ttgcggttcg ccggcgcggg agggaagacg atgggcgctc
     7021 gtttgctgtt tttggtgtcg ttgatcgcca ccggggtggg gacggccatg ccggacaccg
     7081 cgggcgcgca gaccgtcgcc ctgacgccgg agcagatgtc tgcattcaac caacaggtcg
     7141 ttcatgcccg gcaggcgcaa actgtcgtcg gcaagagcga ggcgtgcgtg acattataca
     7201 acgacggatt gcaacgacgc agcagccagc tggagaccgc ccttgccgac aacctgcgtc
     7261 ttgagcgggc ccttgatgtt gagctgatcc ggctcaacga agagagggga cagttctcgt
     7321 cgatcgcctt ggccgagcag cgaaatctcg atgagaagca acgggatctc cagtcggcca
     7381 tcgccgaacg gcaggaacag gaccgcaggc tccaggaatg tcggcgcgcc atgacatttc
     7441 tcgctttcct ctgcgattgg ggcgaccagg ccgttcggga tcttggctgg atgagaaacg
     7501 ttcaggcgga gatcggcggg atcgaggcga ggctcgagag caggcgaaac ggcatgctgg
     7561 acgcggagcg gcgtctcgcc gccagccaca cggccttgac ggaggccgag cgtcagatcg
     7621 cggccagtca gaaagcaata ccgcagattg aggctcaaat cgtgcaggtc aaggcgacga
     7681 tcgccgacct tcatgtgatg atccagagct acaacacgct cctggatgac tttgcgagcc
     7741 tgcttgagca tgcggacgcc gtgactgccg gttcaccgcg cgcccgggag atcatccgtc
     7801 tctcctcaaa catcgaagac ctcgtatcgc gctcgccggc tgctgtcagc gccgccagcg
     7861 ccatcctgcc cgaggacctg aagcaagcct gttccaccca ttgaaaaccg ccccgcccgg
     7921 caaccgggcg cgggcacagg agacgacgat catggcccaa gtgttggagt tcgaaagccg
     7981 caacgcgcag atcgccgcgg acatccggtc cggtgtggac cgtatccggg gcgtcagtcc
     8041 gctagccctg ccagggccgg aggcggccat cgtcaaatcc tacgtccaga aaatccaggg
     8101 cacgtacgac gtcgagcgcg ctaggacaga caccgacaac gccatcgacc tgctctacat
     8161 cgcctacaac acgacgcccc agatggaagg cgacgtccgc aacaccatta gcgccttgat
     8221 gaacaaggtc atcacggcgc agcaggacag cgagcgaacg atgaaggggg ccatgcgcgt
     8281 cgccaacatc atcgtgaact ccctcgacga tgtcttcttt gaatggctgg acgcgaaaga
     8341 gtccgacagc gaggccgacc tcaagcggtt tgtcagcacc gacctcctca ggctcgccac
     8401 cgaaatcaag acgcaggcgc tcaggattcg agacgagctg ctggccgtcg ccagcaccta
     8461 cgacggcatc atccgggaca atgttgcggc gacgtccatc agcgagaagg ctctgagcgt
     8521 ccagctcaag cagaaggccg ccgtcgagaa ggagatgaac gacgcgaccg ccagacgcga
     8581 gcagctggag tccctcgtcg tcgatctgaa agaccaggtc gccaaattcg acaagatggc
     8641 gcgagacttt gaagcgcggg ccagcacggc cgaacagcgg gccttcgtc
//
LOCUS       DASVTM010000096         6338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_168914,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000096 DASVTM010000000
VERSION     DASVTM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168914"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF633_02765"
     CDS             complement(<1..259)
                     /locus_tag="VF633_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX8470012.1"
                     /translation="MFLGTYEKQLDGKRRLLIPQEYRTPDNGAEHGVFCFFSIASDCL
                     EAGGDKLMAEYVSRIEALPFGSDRRTALEETFYAGQRALSYD"
     gene            complement(728..1118)
                     /gene="rnpB"
                     /locus_tag="VF633_02770"
     ncRNA           complement(728..1118)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF633_02770"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1195..1392
                     /locus_tag="VF633_02775"
     CDS             1195..1392
                     /locus_tag="VF633_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470013.1"
                     /translation="MAQPLPQTSNAEDPVAREHRLAWERERLAEAEADLVAGNYIEGD
                     EAIRWLKDELAWARSSADREA"
     gene            complement(1676..2440)
                     /locus_tag="VF633_02780"
     CDS             complement(1676..2440)
                     /locus_tag="VF633_02780"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8470014.1"
                     /translation="MSLIFEDAPSPNFDVRRGLPDMLVLHYTGMASGEAALARLRDPS
                     AKVSAHYLVEEDGRIFALVPEERRAWHAGRGVWQGETDINTASIGIEIVNPGHEFGYR
                     AFPDAQIEAVIALTADIRSRWCITDARIIAHSDMAPKRKHDPGELFPWKRLAQAGHGL
                     WFEPALERIAALGGFLQVGDEGIGVVVLRAGLHRLGYGLQPGSTYDDETAMTVKAFQR
                     HWRQHRVDGVADGETRATLMGVLQLATAESVTGVLN"
     gene            complement(2455..2778)
                     /locus_tag="VF633_02785"
     CDS             complement(2455..2778)
                     /locus_tag="VF633_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470015.1"
                     /translation="MTQNRFASLGFNERRRNSGGGLLAPLMWLAGLVATIAALAVGAV
                     LAVFTAAAVAVIALVASVLVFFAGLAMRARRKVTVRTRRGDDVLEARKVGETWVTYGW
                     ERQGH"
     gene            complement(2903..4684)
                     /gene="sppA"
                     /locus_tag="VF633_02790"
     CDS             complement(2903..4684)
                     /gene="sppA"
                     /locus_tag="VF633_02790"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX8470016.1"
                     /translation="MKQFFLTVLGVFAGLFLFLIVVPIVLVTMAAAAVSSKPTTTANT
                     VLELDLRDGISDQASSSPFAAFSGSSLSVMKIVDTLAQAEKDEHVKVLLLRLPEGGVT
                     PASADEIRQAIRRFRASGKPVIAHSQGFQPIGTAISSYMLGASASELWMQNTANFQVT
                     GFSSDTMFLGRAFEKYGVRADFEQRYEYKNAVNQYTQSDFTEPHREAMTAWMTSVYEN
                     AVGSAAQDRKIAPAALKATIEAGPYSAEQALSLKLIDKIGQAEEAEIEAKRRAGSNSD
                     IVEFGDYASSQGEREGSGANAIAIVGAEGDIVTGRGGGSGFGTSSSIHSDDTAKAIYD
                     AIKDKDVKAIVFRVSSPGGSPEASEQILAAVRAARAAGKPVVVSMGSYAASGGYWISS
                     EADWIVAQPSTITGSIGVFGGKFVLAPALARFGVDLKEISVGGEYSDAFSPTQPFTAS
                     QRAAFSASMDKTYEEFVQRVSTGRHLPVTRVREIARGRVWTGAQALPLGLVDQLGGVT
                     EAIYKARELAKIPATQSVRYKRYPENESPWEALSSVFGVSSEAAHALIAIGGVMSDPQ
                     AEALIRSVETDRMRSRGAVVMADQPLD"
     gene            4902..5258
                     /gene="panD"
                     /locus_tag="VF633_02795"
     CDS             4902..5258
                     /gene="panD"
                     /locus_tag="VF633_02795"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX8470017.1"
                     /translation="MLVTLMKAKLHRATVTQADLDYEGSIAIDGDLLDAAGIFPNEQV
                     DVLNITNGARFTTYAIEAPRGSKVIGVNGAAARLVQKNDLVIVVTYAQMPQEEARQWS
                     PTVVLLDEANNIKRAA"
     gene            5340..6236
                     /gene="hemF"
                     /locus_tag="VF633_02800"
     CDS             5340..6236
                     /gene="hemF"
                     /locus_tag="VF633_02800"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX8470018.1"
                     /translation="MSDVTTDPLALKKAETRAWFEALRDRIHAGFEALEDAAPPELFP
                     GEPGRFVRTPWNRAANSDGSKGGGGVMGMMHGRLFEKVGVHCSTVHGTFPADYAKGVP
                     GASEDPRFFATGISLIAHPWSPRVPAVHMNTRFITTTRSWFGGGADLTPVLDVERTQD
                     APDTIAFHAAMKAACDAHDPSYHPRFKAWCDEYFWLPHRNEPRGVGGIFYDHQDSGDH
                     DADFAFTRDVGTAFLDVYSAIVSGRMTESWTAQERQEQLVRRGRYVEFNLLYDRGTMF
                     GLKTGGNVESILSSMPPSVVWP"
BASE COUNT         1181 a         2092 c         1953 g         1112 t
ORIGIN      
        1 cgtcgtacga aagcgcgcgc tggccggcgt agaaggtttc ctcgagagcc gtgcgccggt
       61 cgctgccgaa aggcagggct tcgatgcgcg agacgtactc ggccatcagc ttgtcgccgc
      121 ccgcttcgag gcagtcagac gcgatggaga agaagcagaa cacgccatgt tcggcgccat
      181 tgtcaggcgt gcggtattct tgcgggatca agagacggcg tttgccgtcc agctgcttct
      241 cataggttcc gagaaacacg ctcttgtccc attcggacct ttcggcccgc ccctgaactg
      301 ccaaacgccc aaatgtcagg cgcgatcacc cccggctcac gtaactggga taacatggga
      361 taagatgggc ctcaatggga ttagaagcca ctttttaacc atgttcggac tgtgatcgcg
      421 atgtcgcgca gttactctag agaacataca cggaacaaag acagtagcgg tggttcgacc
      481 gacaccgtga tgcactgacc tccgcacctt attaatgaag aaattcaacg gccggtggcg
      541 acgcccacgc aggccatcta acaaccccaa cgcgtcgtcc agcacccgtg cagatcatgc
      601 caaagttagg tgtggatcgt cggcagaccg ctcctcgccg aacgctgcgg gtcggggtcc
      661 gcgcaaaatg tccgaggagc cggcaaccat ccggctcaag cagccggcag gcggtacgcc
      721 aggaaaaaat gtcagacggc ctgtaagccg ggttctgtcc cgcgccctga ccgaggtcgg
      781 agagcggtga cgatcattcc tctggaccgc ccattgctgg acggttctcg cgacctaccc
      841 ggacgcctcg ggcggtgaac cctgcgaagc gaacctcgcg cgacgtccct attcggtctt
      901 gctccaggcg gggcttgcca tgccgtccct gttgccaggc acgcggtggg ctcttacccc
      961 accctttcac ccttccctgc gtcgagacgc aggcggtttg ctttctgtgg cgctatccct
     1021 cgggtcgccc cgggcgggcg ttacccgccg ccttttcacc gtggagcccg gactttcctc
     1081 gacgaagcaa gctccgccgc gaccgcccgg ccgtctgacg ggggcgttag tgaccgttcg
     1141 acccgcctgc gtcaacgttc tgtctgacgc ccctcggctg tggtagtgtt ccacatggct
     1201 caacccctgc cccagacttc caacgccgaa gacccggtag cgcgggagca tcgtctggct
     1261 tgggaacgag aacggctcgc tgaagccgaa gccgatctcg tcgccggaaa ctatatcgaa
     1321 ggtgacgagg cgatccgctg gctcaaggac gaactggcgt gggcgaggtc gagcgctgac
     1381 cgtgaggcgt gaagtttcga ctccgccttc tcggcaaatc gcgatctgat ctcgaggcgg
     1441 cgaggctttg gcttactcag ccaggaagcg gtctccgagg cagagcgcga tacgggaaca
     1501 tcaccgaagc cctgcttgat cttgaaagct caccgctgag gtggccatcg agtgaccatt
     1561 cgcagcatcg ggagcgtacg gtcgaaggct atcgtatagt ctaccgtgtt ctcgaacatg
     1621 agcgtcgcgt tgaggttctc cgcatcttcg gcccttacca gaaccgggtc gacggctaat
     1681 tcagcacacc cgtgacgctc tcagcggtcg ccagctgcag cacgcccatc agcgtcgcgc
     1741 gagtttctcc gtcggcgaca ccatcgacgc gatgctgccg ccagtggcgc tgaaacgctt
     1801 tcaccgtcat cgcagtctcg tcgtcgtacg tcgacccggg ctgcagcccg taccccagcc
     1861 gatgcagacc ggcccgcagc accacgaccc cgatcccctc gtccccgacc tgcaggaacc
     1921 caccgagcgc tgcgatccgt tcgagcgcgg gctcgaacca caacccatga ccggcctgcg
     1981 ccagccgctt ccagggaaac agttctccgg gatcgtgctt gcgcttcggc gccatgtcgg
     2041 aatgcgcgat gatgcgggcg tcggtgatgc accagcgcga ccggatgtcg gccgtcaggg
     2101 cgataaccgc ctcgatctgg gcgtcgggaa aggcgcgata gccgaattcg tggccgggat
     2161 tgacgatctc gatgccgatg gaggcggtgt tgatatcggt ctcgccctgc cagacgccgc
     2221 gtcccgcatg ccaggctcgg cgttcttcag ggacgagggc aaagattcgt ccgtcctctt
     2281 ccaccagata gtgagccgaa accttggcgc tggggtcgcg caaacgcgcc agggcggcct
     2341 caccactggc catgccggta tagtgaagga ccagcatgtc cggcaggccg cggcggacat
     2401 cgaaattagg tgaaggcgcg tcttcgaaga tcagactcat gaggctgcga agagctagtg
     2461 gccctgacgc tcccagccgt aggtaaccca ggtctctccg accttgcggg cttccagaac
     2521 atcgtcgcca cgacgggtgc gaacggtcac cttgcggcgc gctctcatag ccagaccggc
     2581 gaagaagacc agcacgctgg cgaccagcgc gatgactgca acggcggcgg ctgtgaacac
     2641 ggccagcaca gcgccgaccg ccagagccgc gatcgtcgcc acgagacccg ccagccacat
     2701 caacggcgcg agcagaccgc cacctgaatt ccggcggcgt tcattgaagc cgagcgatgc
     2761 aaagcggttc tgggtcattc tattcccttt cagagcgctc cgtggaacgc ctgaacctca
     2821 atgtcggtcc atacagatga accgtcaatg accaacaccc tcacagggct gtgtgacgca
     2881 ccagactttg gtgcatttga actcagtcca acggttggtc ggccatcacc acggcgccac
     2941 ggctgcgcat ccgatccgtt tcgaccgacc ggatgagcgc ctcggcctga gggtcggaca
     3001 tgaccccgcc gatggcgatc agggcatgcg ccgcttcgct ggacacgccg aacaccgacg
     3061 agagcgcctc ccacggagac tcattttcag gatagcgttt gtagcgcacc gattgggtcg
     3121 ccgggatttt ggcgagttca cgtgccttgt agatggcttc ggtcacgccg cccaattggt
     3181 cgaccagacc taggggaaga gcctgcgccc cggtccagac ccgaccacgt gcgatctcgc
     3241 gaacgcgcgt gacggggagg tggcggccgg ttgagacccg ctggacgaac tcctcatagg
     3301 tcttgtccat cgaggctgag aaggcagcac gctggctggc cgtgaacggc tgggtcggag
     3361 agaaggcgtc ggaatactcg ccgccgaccg agatttcctt caggtcgaca ccgaaccgcg
     3421 ccagtgccgg agccagcacg aacttgccgc cgaacacgcc aatcgagccc gtaatggtcg
     3481 agggctgggc cacgatccag tcggcctcgg agctgatcca gtagccgcca gatgccgcat
     3541 aggaacccat cgacaccacg accggcttgc ccgcggcacg ggccgcgcgg acggcggcca
     3601 gtatctgttc ggacgcctcg ggcgatccgc cgggcgagga gacgcgaaag acgatggcct
     3661 tcacgtcctt gtccttgatg gcgtcgtaga tggccttggc cgtgtcgtcg gaatgaatcg
     3721 acgagcttgt gccgaagccg ctcccgccgc cgcgtccggt gacgatgtcg ccctcggcgc
     3781 cgacgatcgc gatcgcattc gcgccggagc cctcgcgctc gccctggctc gaggcgtagt
     3841 cgccgaactc gacgatgtcg gagttggaac ccgcccggcg cttggcctcg atctcggcct
     3901 cttcggcctg accgatcttg tcgatcagct tcagcgacag agcctgctcg gcggaatagg
     3961 ggccggcttc gatggtcgcc ttcagcgcag cgggtgcgat cttgcgatcc tgcgcggcgc
     4021 tgccgacggc gttctcatag accgaggtca tccacgccgt catcgcctcg cgatggggct
     4081 cggtgaaatc ggactgcgtg tactggttga cggcgttctt gtactcgtag cgctgctcga
     4141 aatcggcccg gacgccatat ttctcgaacg cccggccgag gaacatcgtg tcggacgaga
     4201 agcccgtgac ctgaaaattg gcggtgtttt gcatccacag ctcagacgca gaggcgccga
     4261 gcatatagct ggagatagcc gtaccgatcg gctggaagcc ctgcgaatgg gcgatgaccg
     4321 gcttgccgga cgccctgaag cgccggatcg cctgacggat ttcatccgcg gatgccggag
     4381 tcacgccgcc ttcgggcaga cgcagcagca ggactttgac atgctcgtcc ttctcggcct
     4441 gggcgagcgt atccacgatc ttcatcaccg aaaggctcga gccgctgaac gcggcgaacg
     4501 ggctggatga ggcctgatcg ctgatgccgt cgcgaagatc gagttccagt accgtattcg
     4561 ccgtcgtcgt gggcttggac gagaccgctg cggccgccat cgtgacaagc acgatcggga
     4621 cgacgatcag gaacagaaac aggccagcga agacgccgag cactgtcagg aagaactgtt
     4681 tcatgggcgg gcggcgatcc gtcgcagtgc cgggagcggc gctaagtcaa ccatagaggc
     4741 catcaggtca ccgtcaaatg aacgtctcgc aagacgcgat cagaaacacg ttgtcgctgc
     4801 ggcgcagcaa aggcgattga tgaccccctt tgaaatccct atatcaaccg cctgcgagcg
     4861 ggggcctaac gccccgcgtc atgactggac cgcgcactcc aatgctggtc acgctgatga
     4921 aggccaaact gcaccgcgct acggtgacac aggccgatct cgattatgaa ggctcgatcg
     4981 ccatcgacgg cgatctgctg gatgctgcgg gcatcttccc gaacgaacag gtcgatgtgt
     5041 tgaacatcac caacggcgca cgcttcacca cttacgccat cgaggctccc cgtgggtcga
     5101 aggtcatcgg cgtcaacggc gccgcagcgc gtctggttca gaagaacgac ctcgtcatcg
     5161 tggtgactta cgcccagatg ccgcaggaag aagcccgcca gtggtcgccc accgtcgtgc
     5221 tgctcgacga agccaacaac atcaagcgcg ccgcctgatc tgaccggctc gacggggtca
     5281 ctgacccttg gacagtttgc accggtgacg atccctccca tccgtggttc aagccacgca
     5341 tgagcgacgt gaccaccgac ccgctggccc tcaaaaaggc cgagacccgc gcctggttcg
     5401 aggccttgcg ggaccgtatt catgcgggct tcgaagccct cgaggacgcg gctccccccg
     5461 agctcttccc cggcgagccc ggccgtttcg tccgcacgcc ctggaaccgc gctgccaatt
     5521 ctgacggaag taaaggcggc ggcggcgtca tgggcatgat gcatggccgg ctgttcgaga
     5581 aggtcggcgt ccactgctcg accgtccacg gcaccttccc cgcagactac gccaaaggcg
     5641 ttcccggcgc ctccgaggat ccccgcttct tcgccaccgg catcagcctg atcgcccacc
     5701 cttggagccc gcgtgttccg gcggtgcata tgaacacccg cttcatcacc acgaccagaa
     5761 gctggttcgg cggcggcgcc gatctgactc cggttctcga tgtcgaacgg acgcaggacg
     5821 cgccggacac gatagccttc cacgccgcca tgaaggccgc ttgcgacgcc cacgatccaa
     5881 gctatcaccc gcgcttcaag gcttggtgcg acgagtattt ctggctgcct caccgcaacg
     5941 agccgcgtgg cgtcggcggc atcttctacg accatcagga cagtggcgat cacgacgccg
     6001 acttcgcctt cacccgcgac gtgggcacgg ccttcctcga tgtctattcc gccattgtgt
     6061 ccggtcgcat gaccgagtct tggaccgcgc aggagcgtca ggaacagctg gtccgccggg
     6121 gccgctacgt cgagttcaac ctgctctacg accggggcac gatgttcggg ctcaagaccg
     6181 gcggcaacgt cgagtcgatc ctcagctcga tgcccccctc cgttgtctgg ccctaagtct
     6241 cagcgcggcg ccgtgagcag cgctgcggta tgccgccagt gaccgtcgcg cgtgcgctcc
     6301 agaacggcat ggaccggcgt cgcgccgggc tcctgata
//
LOCUS       DASVTM010000097         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_223239,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000097 DASVTM010000000
VERSION     DASVTM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223239"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF633_02805"
     CDS             complement(<1..231)
                     /locus_tag="VF633_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX8470019.1"
                     /translation="MTDALPAASLLKSQRFRHAREDDWKRLERLMEKAEKGQVGKLTD
                     EEILAVPVLYRSTLSALSVARETSLDLGLIAYL"
     gene            complement(228..1556)
                     /locus_tag="VF633_02810"
     CDS             complement(228..1556)
                     /locus_tag="VF633_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8470020.1"
                     /translation="MIYPTRRAVLAIAAGLPVAMVVGVLTPAGWLIAPVWIAVVGLLL
                     GLDALMGGSRTELTLDTPATLFPAVARPAQADFTVRFGKARFGWGVAPSHAEAALAAD
                     KRLGFADPVAHARVHDETVHFVFPFTPERRGAALLQRLWIRWTGPMGLTWKQKIFELD
                     QSTPVTTDLQSVRDDAVRLFARDALFGAKTQIELGEGSEFQALRDFQPGMDRRAIDWK
                     QSARHSALLAKEFRTERNHHVIMALDCGRVMSEPVGGAPRIDRAINASLLLAWACLKS
                     GDRAGFFAFDSIPRLSTGVASGLDAFRSLQLVAGRIDYSTHETNYTLALTTLAGQLRR
                     RSLVVVFTDFTDSTAAELMLDSLGRLLRRHLVLFVVLRDEELEMLTEVEPVQAEDVAR
                     AVVAGDLLLERDAVVTRLRRMGVHIVDAPADRIGPAVINAYLDLKRRDLL"
     gene            complement(1553..>2373)
                     /locus_tag="VF633_02815"
     CDS             complement(1553..>2373)
                     /locus_tag="VF633_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8470021.1"
                     /translation="PGTAKTFMAQCFARTLKLDYGRIQFTPDLMPGDIIGANLFNFQT
                     SSFTLTRGPIFCELLLADEINRTPPKTQAALLEAMQERQITIDGVSHQLSDRFTVVAT
                     QNPIEQQGTYPLPEAQLDRFLFKHVLDYPTIEQERAIVSAHGARSGQMDPVALGVQAV
                     ADRATIDAAVATVANVRLTDEVIGYIVDLIRATRDSADTDTGASPRAGAMLAVAARAR
                     AALDGRDYVIPDDVKVLAMPTLRHRLLLSPAAEIEGRKVDQVLKSLVEQVAAPR"
BASE COUNT          436 a          755 c          818 g          364 t
ORIGIN      
        1 gagataggcg atgagcccca gatccagcga ggtctcgcgc gccaccgaca gggccgacag
       61 cgtcgagcga tagagcaccg gcacggcgag gatttcctcg tcggtcagct tgccgacctg
      121 acccttctcg gccttttcca tcagccgttc gagacgcttc cagtcgtctt cgcgcgcatg
      181 acgaaagcgc tggctcttga gcaaactcgc ggcaggcagg gcgtcggtca cagcaggtcc
      241 ctccgcttca gatcaaggta tgcgttgatg actgccgggc cgatgcggtc agcgggagcg
      301 tcgacgatat gcacacccat gcggcgaaga cgggtgacga cggcgtcgcg ctcaagcagc
      361 aggtcgccgg ccaccacggc gcgggcgacg tcctcggcct gcacaggctc gacctcggtc
      421 agcatctcca gctcctcgtc gcgcagaacg acgaacagca ccagatggcg gcgcagcaac
      481 cggcccagcg agtcgagcat cagttcggcg gcggttgaat cggtaaaatc ggtgaagacg
      541 acaaccagcg agcggcgtcg gagctggccc gccagagtgg tcagggccag ggtgtaattg
      601 gtttcgtgcg tcgaatagtc gatgcgacca gcgaccagct gcagcgaacg gaaggcgtcc
      661 agacccgacg ccacgcccgt tgagaggcgg gggatggaat cgaaggcgaa gaatcccgcc
      721 cggtcgccgg acttcagaca ggcccaggcc aacaacaggc tggcgttgat ggcgcgatcg
      781 atccggggcg cgccgccgac cggctcgctc atcacccgac cgcagtccag cgccatgatg
      841 acgtggtgat tacgctcggt tcggaattcc ttggccagca gggccgaatg acgggcggat
      901 tgcttccagt cgatggcgcg gcgatccatg ccgggttgga aatcgcgcag ggcctggaac
      961 tccgagcctt cgcccaactc gatctgggtc ttggccccga aaagggcgtc gcgggcgaac
     1021 agccgcaccg cgtcgtcgcg cacagactgc aaatcggtgg tgacgggcgt cgattgatcg
     1081 agctcaaaga tcttctgctt ccacgtcagc cccatcggac ccgtccagcg gatccacagg
     1141 cgctggagca gagctgcacc gcgacgctcg ggcgtgaacg ggaagacgaa gtgaaccgtc
     1201 tcgtcgtgca ctctggcgtg ggcgactgga tcggcgaaac ccagcctctt gtcggccgca
     1261 agcgcggcct ctgcgtggga gggtgcgacg ccccagccga accgggcctt accgaaccgg
     1321 acggtgaaat ccgcctgcgc cggtcgagcg acagcgggaa aaagggtcgc gggcgtgtcg
     1381 agcgtcagtt cggtgcgcga tccgcccatc aacgcgtcga gaccgagcag caggcccacg
     1441 acggcgatcc agacaggcgc gatcagccag ccggcaggcg tcaggacgcc aaccaccatg
     1501 gccacgggca agcccgccgc tatcgccaga accgctcgcc gggtggggta gatcaccggg
     1561 gcgccgcgac ctgttccacc agcgacttga gcacctgatc caccttccgg ccctcaatct
     1621 cagcggccgg tgacagcagc aggcggtgac gcagggtcgg cattgccaac actttcacgt
     1681 cgtccggaat gacgtagtcg cgaccatcga gagcggcgcg ggcgcgggcc gcgaccgcca
     1741 gcatggcgcc ggcccgaggc gaagcgccgg tgtcggtgtc ggcagagtcg cgtgtcgccc
     1801 ggatcaggtc gacgatatag ccgatgacct cgtcggtcag gcgcacattg gcgacggtcg
     1861 caacggccgc gtcgatggtc gcgcggtctg caacggcctg aaccccaagg gcgacaggat
     1921 ccatctggcc cgagcgtgcg ccatgcgccg agacgatggc gcgttcctgc tcgatcgtcg
     1981 gataatcgag cacgtgtttg aacaggaacc ggtcgagctg cgcctcgggc agcggatagg
     2041 tgccctgctg ctcgatcggg ttctgggtgg cgacgacggt gaagcggtcg ctcagttggt
     2101 gcgacacgcc gtcgatagtg atctggcgct cctgcatggc ttccagcaag gcggcctgcg
     2161 tcttgggcgg cgtccggttg atctcgtcgg ccagcagcag ttcgcagaag atcggcccgc
     2221 gcgtcagggt gaagctggac gtctggaaat tgaacagatt ggcgccgatg atgtcccccg
     2281 gcatcaggtc gggcgtgaac tggatgcggc catagtccag cttcagggtc cgggcgaagc
     2341 actgagccat gaaggttttg gcggtgcccg gcg
//
LOCUS       DASVTM010000098         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_381800,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000098 DASVTM010000000
VERSION     DASVTM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381800"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF633_02820"
     CDS             <1..542
                     /locus_tag="VF633_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470022.1"
                     /translation="SGIKTFKRDLGMLISSADAQVFYHLDVPLVSLWQLRGQKRVWVY
                     PVTEPCVSDEQLERVVLKETAEQLEFRPEMDAEAEVFDLTPGKMVTWRQNAPHRIENG
                     PMLNVSLSVEFMTPAALMRADVLYANGVIRKAGARPRIQSAPHPTALAKIAFARAAKM
                     SGVTKTFERSLPETFRLQS"
     gene            complement(539..721)
                     /locus_tag="VF633_02825"
     CDS             complement(539..721)
                     /locus_tag="VF633_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470023.1"
                     /translation="MPPRRTLSQCREKISEMIGEAVVEDDDARREGLLTLADLWVEVA
                     ERRREDEMPSPSGHCD"
     gene            complement(817..1371)
                     /locus_tag="VF633_02830"
     CDS             complement(817..1371)
                     /locus_tag="VF633_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8470024.1"
                     /translation="MTDIAAPVAERLLVLVEDDEAFARTLKRSFERRDYKVVTATNHD
                     ELVAVLADHRPAYAVVDLKLAGGASGLTCVQTLHAADPTTIIVVLTGFASIATAVDAI
                     KLGASQYLAKPSSTDDIEAAFSRVEGNPDTALTARPTSIKTLEWERIHETLADTGFNI
                     SEAARRLGMHRRTLARKLAKSQVT"
     gene            complement(1368..>1792)
                     /locus_tag="VF633_02835"
     CDS             complement(1368..>1792)
                     /locus_tag="VF633_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470025.1"
                     /translation="LVAGWGATHAGVEVAYRDDLGEDLTVVSDPALLQVLVNVLDNAS
                     EAGATAVVILAERQDGDLILTVSDNGPGFAAAVLERFGQPYQSTKARPGAGLGLFLLL
                     NVLRKLGGGATARNRKDGGAVVTLRLPLTAIAYPGKPT"
BASE COUNT          298 a          555 c          611 g          328 t
ORIGIN      
        1 agtcgggcat caagaccttc aagcgcgatc tggggatgct gatctcgtcg gccgacgctc
       61 aggtctttta tcacctagac gtgccgctgg tgtcgttgtg gcagttgcgt ggacagaagc
      121 gggtctgggt ctatccggtc accgaaccct gtgtcagcga tgagcagctt gaacgcgtcg
      181 tgctcaagga aacggccgaa cagctcgagt tccggcccga gatggacgcc gaggctgagg
      241 tgttcgatct gacgccgggc aagatggtca catggcgcca gaacgctccg caccggattg
      301 aaaacgggcc gatgttgaac gtctcgctga gcgtggaatt catgacaccg gcggccctga
      361 tgcgcgccga cgtgctgtat gcgaatggcg tcattcgcaa ggcaggcgct cgtccgcgca
      421 tccagtcggc gccgcatccg acggcgctgg ccaagattgc cttcgcccgg gcggccaaga
      481 tgtctggcgt gacgaagact ttcgagcgca gcctgcccga gaccttccgg ctgcaatcct
      541 agtcgcagtg gccggaaggg gagggcattt catcctccct ccgtcgctcg gcgacctcga
      601 cccaaaggtc cgccagcgtc agcagacctt cgcggcgcgc atcgtcatct tcgacgacgg
      661 cttcaccgat catctcgctg atcttctcac gacactggga cagggttctg cggggaggca
      721 ttgtaatcag acggtttcgg ccccgatcaa ccgccgtctt gcactgatgt tccgtatggc
      781 ggcgctcgga atacgggcat ttcgccatag gggagatcag gtcacctgac tcttggccag
      841 cttccgcgca agggtccgtc ggtgcatgcc aagacgacgc gcggcctccg aaatgttgaa
      901 gccggtgtcc gccagggttt cgtgaatccg ctcccactcc agtgtcttga tcgaggtcgg
      961 gcgggcggtc agcgccgtgt ctggattgcc ttcgacccgg ctgaacgcag cctcgatgtc
     1021 gtccgtgctg gaaggtttgg cgaggtattg cgacgcgccc agcttgatgg cgtcgacggc
     1081 ggtggcgatg ctggcgaacc cggtcaggac cacgataatg gtggtggggt cggcggcgtg
     1141 cagcgtctgg acgcaggtca ggcccgaagc gccgcccgcc agcttcagat cgactacggc
     1201 ataggctggc cggtggtcgg cgaggacggc gaccagttcg tcgtggttgg tcgcagtcac
     1261 caccttgtag tctcggcgtt cgaacgagcg cttgagcgtg cgggcgaagg cttcgtcatc
     1321 ctcgacgagg accagcaggc gttcggcgac gggtgcggcg atgtcggtca tgtcggcttt
     1381 cccggatagg cgatggcggt cagcggcagt cgcagggtga ccacggcgcc gccgtccttg
     1441 cggttgcgag cggtcgcgcc gccgccgagc ttgcgcagca cattgagcag caggaacagg
     1501 cccagacccg cgcccggccg ggccttggtc gattgatagg gttggccgaa ccgctccagc
     1561 actgcggccg caaaacccgg gccattgtcg ctgaccgtca ggatcagatc gccgtcctgc
     1621 cgttcggcga ggatgacaac ggcggtggcc ccggcctcgg aggcgttgtc gagcacattg
     1681 accagcacct gcagcagggc gggatcggac accacggtca ggtcttcgcc gaggtcgtcg
     1741 cgataggcga cctcgacacc cgcgtgggtc gcgccccagc cggcgaccag at
//
LOCUS       DASVTM010000099         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_966996,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000099 DASVTM010000000
VERSION     DASVTM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966996"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="VF633_02840"
     CDS             <1..1947
                     /locus_tag="VF633_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX8470026.1"
                     /translation="NDPVYRSFRRPGRTEKIVGGVALLLLAAIVIFLLLFDWNWLSGP
                     IGRWASAKYDREIALQGDLDVNLFSWTPSVIVRDLKFGGPQWAKEKDTADIAQIEASV
                     RLRKLFAGQIEMPLLSLTRPDVTLIATKDGKNSWTLGPPKPDDGKGMKLPVIQQLIIA
                     DGHITLDEQKRGLTLDADINARETANDGDAGFILNGRGSVNKSPLTLRIEGGPFINIR
                     RNKPYHFTANVAGAGSRLVADGSITRPFDLGQFQSTLSLQGQDLNDLYLLTGITLPNT
                     PPYRLSGALTRDDSLWTFNDFSGRVGASDLSGDLKVETSGRMRVDAQLASRKLDINDL
                     SAILGAKTQTNAAGTETTTVSSGAPGKLLPDATLQVERLRSMDGSLTYRANSVKANEL
                     DIRAVQLGAKLEKGILNLDPVAFNFNRGELNGTARINATRDTPYSAIDFRLSGYPLES
                     IVPAKNGSTPISGRALGRARLEGSGSSIHRFAAGSKGSISLVVPQGQMRSAFAELLGI
                     NASAGLLKLLRGDQSTSNIRCAVADFDVAGGVARARTFVLDTDVVLAKGTGSIDLGQE
                     TLNLRIDGDSKKPRLLRLWTPILVRGPLTKPRIGVDTASLVTQGGLTALVGAVVAPVA
                     ALFAFVDPGLAKDANCGALIASAR"
     gene            2034..2894
                     /locus_tag="VF633_02845"
     CDS             2034..2894
                     /locus_tag="VF633_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8470027.1"
                     /translation="MTRRGILALTLGSLTAACSPLGLLNTLGPRDRGVGRVAHDLAYG
                     DDPRQKFDLYGPQWTAGQPTLPVIVFFYGGGWDSGSRSLYGWAAQALAARGFMVALPD
                     YRIVPQVVFPAFIEDAARATARVAEVIGQYGGDPARLGVAGHSAGAHLAMMIALDRRY
                     MEAVGQPDLIKAAAGLSGPYEFLPLDVASSINAFGRAPDPTMTQPVTFVRADAPPLWL
                     GHGTDDTVVHDDDTIILNRRMHTVGGRSEAKLYPGLNHADMIATFSPLFRKKAPVLDD
                     VSAFFHRELG"
     gene            3088..4533
                     /locus_tag="VF633_02850"
     CDS             3088..4533
                     /locus_tag="VF633_02850"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX8470028.1"
                     /translation="MTAQMTAGAALDRLETLYTDSVANLRDAVRHFLVTGERADTEAR
                     AKGLFSYPSLKISWFGDRPINLPIRAYARMSRQGVYATTVTRPDLFRSYLQEQLTLLE
                     QDYGATFTVEPSEQEIPFPYVLDGSDVVLNPSQTAAIARYFPTTDLAHIGDEISDGLF
                     AADEDFPLSQFDGLRTDFSLARLRHYTGTPVEDVQSYILFTNYNRYVDDFVRWACEQL
                     RDPGSPYETLSCAGGVVITRETADPETAIMDAAWKKHQMPAYHLTAPGNAGITLVNIG
                     VGPSNAKTICDHLAVTRPHAWLMIGHCGGLRPSQTIGDYVLAHAYLRDDHVLDAVLPP
                     DIPIPSIAEVQRALYDAAKMVSGAPGEEVKRRLRTGTVVTTDDRNWELRYSKSALRFN
                     QSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGSISEHL
                     QIGIQAIELLRQEGSKLHSRKLRAFDEPPFR"
     gene            complement(4530..>4879)
                     /locus_tag="VF633_02855"
     CDS             complement(4530..>4879)
                     /locus_tag="VF633_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470029.1"
                     /translation="LAPGGWLLWGDMVWLAAPSEPLKQLIELTNTYADHEGWQVAARD
                     AGFKVVTAHVSSPELWNRYRTTMQDAVEAWLEANPEHPDAASIRSRAHQLKLMFDFGE
                     GTLGFGLYLLKAP"
BASE COUNT          877 a         1717 c         1428 g          857 t
ORIGIN      
        1 aacgatccgg tttaccgctc ttttcgccgc cccgggcgaa cagaaaaaat cgtcggcggg
       61 gtcgctctgc ttctgctcgc tgcgatcgtc atcttcctgc tgctgttcga ctggaactgg
      121 ctgagcggtc caatcggacg ctgggcctca gcgaaatatg accgcgagat cgcgcttcag
      181 ggcgacctcg acgtgaacct gttcagctgg accccgtcgg tcatcgtgcg tgacctgaag
      241 ttcggcggtc ctcagtgggc caaggaaaag gacacggccg acatcgccca gatcgaggcc
      301 tccgttcgcc tgcgcaaact gttcgcgggc cagatcgaaa tgccgctcct gtccctgact
      361 cgcccggacg tcaccctgat cgcgacgaag gacggcaaga acagctggac cctcggtccg
      421 cccaagcccg acgacggcaa gggcatgaag ctgcccgtga ttcagcaact gatcatcgct
      481 gacggtcaca tcactctgga tgagcagaag cgcggcttga cgctggacgc cgatatcaac
      541 gcgcgcgaga ccgccaacga cggcgacgcc ggcttcatcc tgaacgggag gggcagcgtg
      601 aacaaatctc cactgacgct gcggatcgag ggcggcccct tcatcaacat ccgccgcaac
      661 aagccctatc atttcaccgc caatgtcgcc ggcgcgggct cgcggctggt ggctgacggg
      721 tccatcaccc gacctttcga tctcggccag ttccagtcga ccctgagcct gcaaggtcag
      781 gacctgaacg atctctatct gctgaccggc atcaccctgc cgaatacccc gccctatcgc
      841 ctgtccggcg ccctgacgcg ggatgacagc ctctggacct tcaacgactt ctcgggccgg
      901 gtcggcgcat ccgacttgtc cggcgatctg aaagtcgaga cgagcggacg aatgcgcgtt
      961 gacgctcagc tcgcctcccg caagctcgac atcaatgatc tcagcgccat cctcggcgcc
     1021 aagacccaga ccaacgccgc cggaaccgag acaacgaccg tcagctcggg cgcgccggga
     1081 aagctgttgc cggacgccac cctgcaggtg gagcgcctcc gctcgatgga tgggtctctg
     1141 acctaccggg cgaactccgt gaaggccaac gagcttgaca tccgggctgt tcagctcggg
     1201 gcgaagcttg aaaagggcat cctcaatctc gacccggtcg ccttcaactt caatcgcggt
     1261 gaactcaacg gcacggcccg gatcaacgcc acccgcgata caccttacag cgctatcgac
     1321 ttccgtcttt cgggctaccc gctggaatcg atcgttccgg ccaagaacgg ctcgacgcca
     1381 atcagcggca gggcgctggg ccgggcgcgg ctggagggat ccggcagctc gatccaccgg
     1441 ttcgccgccg gatccaaggg cagcatcagc cttgtggtgc cgcaaggcca gatgcgatcc
     1501 gccttcgccg aactgctcgg catcaacgcc agcgccgggt tgctgaagct gctgcgcggc
     1561 gaccagtcga ccagcaacat ccgctgcgcc gtcgccgatt tcgacgtcgc cggcggtgtc
     1621 gcccgggcgc gcaccttcgt tctggacacg gatgtcgtgc tcgccaaggg caccggctcg
     1681 atcgaccttg gccaagaaac cctgaacctg cgcatcgatg gcgacagcaa gaagccccgc
     1741 ctgctgcgcc tgtggacgcc gatcctcgtg cgcggccccc tgacgaagcc gagaatcggc
     1801 gtcgatacgg cgtcactggt cacccagggt ggtctgacgg cgctggtcgg cgcggtcgtt
     1861 gctccggtcg cggccctttt cgccttcgtc gatcccggtc tggccaagga cgccaactgc
     1921 ggcgctttga tcgctagtgc tcggtgaggc gccagaaaat tccggtcgca cttgagccga
     1981 cagtcgccga cgtgcgtatc tggacgccta ctttcttcgc ctgacgagac tccatgaccc
     2041 gcagaggcat cctcgccctc acactgggct ccttaaccgc cgcctgttct ccgttgggct
     2101 tgctcaacac gctcggccca cgtgatcgag gtgtcggacg ggtggcgcac gatctggcct
     2161 atggcgatga tccgcgccag aaattcgatc tctatggtcc gcaatggacg gcgggtcagc
     2221 cgaccctgcc ggtcatcgtc ttcttctacg gcggcggctg ggattcagga tcccgctccc
     2281 tgtacggctg ggctgcgcag gccttggcgg cacgcggttt catggtcgcc ctgcccgact
     2341 ataggatcgt gccacaggtc gtctttcccg ccttcatcga ggacgcggcc cgcgctaccg
     2401 cccgggtggc tgaagtcatc ggccagtacg gcggagaccc cgcacgcctc ggcgtcgccg
     2461 gccattcggc gggcgctcac cttgcgatga tgatcgcgct tgatcggcgc tacatggagg
     2521 ctgtcgggca gccggacctc atcaaggccg ccgccggtct gtcgggaccc tacgagtttc
     2581 tgccgctcga cgtcgcctcg tcgatcaacg cctttggtcg cgcaccggac ccgacgatga
     2641 cccaacccgt caccttcgtg cgcgccgacg cccctccgct gtggcttggt cacggcacgg
     2701 acgacaccgt ggtccatgac gacgacacca tcatcctgaa ccggcgaatg cacactgtcg
     2761 gcggccgcag cgaagccaag ctttatcccg gcctgaacca cgccgacatg atcgcaactt
     2821 tctcgcccct gtttcggaag aaggcgcccg tcctcgacga cgtcagcgcc ttcttccatc
     2881 gagaactggg ttagacgcag gtcagccatc cagcgtccgg ccgcatcgcg gagccatttg
     2941 cctctcagtt gcgctcaagc agcaaggctc cgcgagccga gcgttagcgc ggcggcgtaa
     3001 gccgccccaa agcaacagac gcaaagcaaa gcttcaccgt ctcgccaagg tcatgttctt
     3061 ccgctagatg aggccatgac agaaacaatg acagcccaaa tgacggctgg ggcggctctg
     3121 gaccgcctcg agaccctcta caccgactcc gtcgccaacc tgcgcgacgc ggttcgccac
     3181 ttcctcgtca caggcgagcg ggcggacacc gaggcccgcg ccaagggcct cttctcctat
     3241 ccttcgctca agatcagctg gttcggcgat cgcccaatca acctgcccat ccgcgcctat
     3301 gcccggatgt cacgtcaggg cgtgtacgcc accacggtca cccggcccga cctcttccgg
     3361 tcatacctgc aggaacaact gacccttctc gaacaggact acggcgcgac cttcacggtc
     3421 gagccgtcgg agcaggaaat ccccttcccc tatgtgctgg acgggtcaga cgtggtcctc
     3481 aacccgtcgc agacagcggc catcgcccgc tatttcccga ccacggacct ggctcatatc
     3541 ggcgatgaaa tctcggacgg cctgttcgcc gcggacgagg atttcccatt gtcgcagttc
     3601 gacgggctgc ggacggactt ctcgctggct cgcttgcggc actacaccgg gacccctgtc
     3661 gaggatgtcc agtcctacat cctgttcacc aactacaacc gctacgtcga tgatttcgtg
     3721 cgctgggcct gcgagcagtt gcgcgatccg ggcagccctt acgaaaccct gtcctgcgcc
     3781 ggcggtgtgg tcatcacccg cgaaaccgca gatccggaaa cggccatcat ggacgccgcc
     3841 tggaagaagc atcagatgcc cgcctaccac ctcacggcgc cggggaatgc aggtatcacg
     3901 ctggtcaaca tcggtgtcgg gccatccaac gccaagacca tctgcgacca cctggccgtc
     3961 acccggcccc acgcctggct gatgatcggt cactgcggcg gcctgcgccc cagccagacg
     4021 atcggcgact atgtgctggc tcatgcctat ctgcgcgatg atcacgtgct ggacgcggtg
     4081 cttccgccgg acatcccgat cccgtcgatc gccgaggtcc agcgcgccct ctacgacgcc
     4141 gccaagatgg tctcgggcgc tccgggcgaa gaggtcaaac gccgcctccg caccggaacg
     4201 gtcgtcacca ccgacgaccg gaactgggag ctgcgctact cgaaatccgc cctgcgcttc
     4261 aaccagtcgc gcgccgtggc catcgacatg gaatccgcga ccatcgccgc ccagggctac
     4321 cgcttccgcg tcccctacgg cacgctgctg tgcgtctcag acaagccgct gcacggtgaa
     4381 atcaagctgc cgggtcaggc caatcgcttc tatgaaggct cgatctcgga acacctccag
     4441 atcggcattc aggcgatcga actcctgcgt caggaaggct cgaagctgca ttcccgcaag
     4501 ctgcgcgcct tcgacgagcc gcctttccgc taaggcgctt tcagcaggta aagaccaaat
     4561 ccgagcgtcc cctcgccgaa gtcgaacatc agcttcagtt gatgcgcgcg gcttcggatc
     4621 gaggcggcgt cgggatgttc tgggttggcc tcaagccatg cctcgaccgc gtcctgcatc
     4681 gtcgtgcgat agcgattcca cagctccggc gacgatacat gagcggtaac aaccttgaag
     4741 cccgcgtcac gcgcggcgac ttgccagccc tcatggtcgg cataggtatt ggtcagttcg
     4801 atcagctgct tcagcggctc cgacggcgca gccagccaga ccatatcgcc ccatagcagc
     4861 cagccgcccg gcgccagat
//
LOCUS       DASVTM010000100         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_327887,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000100 DASVTM010000000
VERSION     DASVTM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327887"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1411)
                     /locus_tag="VF633_02860"
     CDS             complement(185..1411)
                     /locus_tag="VF633_02860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8470030.1"
                     /translation="MRIAVVMPRGSVMGRDKANSMEMVARTLLQHSRHKATVRIICDA
                     GADVPALSDLMTVPAGLSKTKRADAVAELLAGFDPDYIEYHQQLESAASLARRYPTKI
                     NVLYRHTRIKQPKNLLDRMRYGARLKAFDKLIFVSKAAGAEFIGDYPALADRVSSICN
                     PIEIDAWKASPDKREKLILFSGRAMEEKGLEPFCEALAAVLDTAPDWRGALMLGDWDR
                     HEAWAGPRIAALARFGDRVEIHKSASLPQVQAVTRRAAIAVTPSFVAEALGLSALEAH
                     AAGAALISSGRGGLREASGDHAVYVDPPHAAGLTEAMSALVTNANRRIAMARAGQKFV
                     AAVHAPAVRAAQLDDLRETLVAERAQSAVREAVRARTPLLQRIAAALSGRPWSVQPAS
                     WTFRTVVREHVEWIRA"
     gene            complement(1414..2394)
                     /locus_tag="VF633_02865"
     CDS             complement(1414..2394)
                     /locus_tag="VF633_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470031.1"
                     /translation="MYDIWRVGFVRRSLSSVTATAGVDASEVTWLPNSGPFQYVADPF
                     GIERDGVLTVFVEAFDYRVRRGEIHFYQYSADNALTGHGVALTAPIHLSYPSLIEDGG
                     ELYMLPEGYKAGGLTLYRCVRFPDVWEPAQQIMDVPAIDATVVKHGDKWWAFYALPGP
                     EDRAMRELHVAWSETLTGPWIPHAANPVRSGFDASRPGGTAFDHDGALHLPVQDCSTT
                     YGAAINLLRIDVLTPEAFEATLVHRFESTGLLTDHEDGFHTLSGMGDVTFVDVKSIRH
                     SPSEGWIKAQFKVRRLLGLNAPRRRSASAVVSRPINHLPPVSTVAARVGG"
     gene            2553..3077
                     /locus_tag="VF633_02870"
     CDS             2553..3077
                     /locus_tag="VF633_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8470032.1"
                     /translation="MTAQAAHPLIGAYFEHRAALGRFLQARLGASGDVEDLLQDLYLK
                     VAALDPAIEVRDARAFLYRLASNQMMDRWRSGQRSVARDAAWRLANHAVGETEDLNDA
                     PSAEAVVIGRQKLAALTAAVSRLPDKTRTVFSLHKFEGFSYAEVAGRLGISRSSVEKH
                     MMDALRVLSARIRS"
     gene            3187..>3769
                     /locus_tag="VF633_02875"
     CDS             3187..>3769
                     /locus_tag="VF633_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX8470033.1"
                     /translation="MTTSQPDIEEQALEWFVRLSDVDAPASAWMSFQDWLEADEAHGA
                     AYEAVERTWVVLDGVVPTVAVLPVAANDQSSPARASNARRRRPVWLLPSFAAAAAAAM
                     VIGIWRQISAVAPVESYHTDLEPRTVVLSDGSRVVMNRHSDLSVRIRRDNRTVTLADG
                     EAAFDVAHNPDRPFVITAGDHSVRVLGTAFNVLN"
BASE COUNT          684 a         1241 c         1196 g          648 t
ORIGIN      
        1 cgaggatgcg tctggctcgc atcgtcttgc gtttggccag accggcctcg ccgagaaaca
       61 ggcgggtgaa gaatgttcct gcgcccacgg cgacctccga tcgtcaccgc cggacgacct
      121 tcgccacggc gcagcgcaat aagacgcgcc ttcactcaac gggtgatgat cggaccggac
      181 gaggtcaagc cctgatccat tccacatgct cgcgaacgac cgtccgaaag gtccagctcg
      241 cgggctgaac cgaccatggc cgtccgctca gcgcggctgc gatccgctgg agaagcgggg
      301 tcctggctcg aacggcttcg cgcacagcgg attgcgcacg ttccgcgacg agggtctcgc
      361 ggaggtcgtc aagttgggct gcgcggacgg ctggagcatg caccgcggcc acgaatttct
      421 ggcccgcacg cgccatcgcg attcggcggt tggcgttggt caccaatgcc gacatcgcct
      481 cggtcagacc ggcggcatgg ggcggatcca catagacggc gtggtcgccg ctggcttcgc
      541 gcaggccgcc tcgacctgag gaaatcagcg cggcgcccgc tgcatgagct tccagagcgg
      601 acaggcccaa ggcctcggcc acgaacgacg gcgtaaccgc gatcgcagcg cggcgggtca
      661 cggcctgaac ttgcggcaag gacgccgact tgtgaatctc cacgcggtcg ccgaaccggg
      721 ccaaagccgc gatccggggg cccgcccacg cttcatggcg gtcccagtcg cccagcatca
      781 atgcgccacg ccagtcgggc gccgtgtcga ggacggcagc cagtgcctcg cagaacggct
      841 ccagtccctt ctcttccatg gctcggccag aaaacaggat cagcttctcg cgcttgtcag
      901 ggctggcctt ccacgcatcg atctcgatgg gattgcagat cgaggacaca cgatccgcca
      961 gcgccggata gtctccgatg aactctgcac ccgcagcctt gctgacgaag atcagcttgt
     1021 cgaacgcctt gagccgcgcg ccataacgca tgcgatccaa caggttcttc ggctgtttga
     1081 tccgcgtatg tcgatacagg acgttgatct tcgtcgggta gcgccgggcc aggctggcgg
     1141 ctgattccag ctgctggtga tactcgatgt agtccggatc gaacccggcc agcagttcgg
     1201 cgacggcatc ggcccgcttc gtcttgctca ggcccgcagg cacggtcatc aaatcagaca
     1261 gggctggaac gtccgctccg gcgtcgcaga tgatgcgaac ggtggccttg tgccggctat
     1321 gctgcagcag ggtgcgagcg accatctcca tcgaattggc cttgtcgcgg cccatcacgc
     1381 tgcctcgcgg catcaccacg gcgatacgca tcactatccc cctacgcggg ccgcgaccgt
     1441 cgagacgggc ggcagatgat tgattgggcg gctaaccacc gcgctcgccg accggcggcg
     1501 cggagcgttc aatcccaaca gccgacgaac cttgaactgg gccttgatcc agccttccga
     1561 cggcgagtgg cggatgctct tcacgtctac gaaggtgacg tcgcccatgc ccgacagggt
     1621 gtggaagccg tcttcgtgat cggtcagcag acccgtcgac tcgaaccgat gcaccagcgt
     1681 cgcctcgaag gcctcaggcg tcaggacgtc gatacgcagc aggttgatcg ccgcgccgta
     1741 ggtcgtcgag cagtcctgca ccggtagatg cagggcgccg tcgtggtcga aggccgtccc
     1801 acccggccgc gaggcatcga agcccgaccg cacgggattg gccgcatgcg ggatccacgg
     1861 cccggtcagg gtctcggacc atgcgacatg aagctcgcgc atcgcgcggt cttccggccc
     1921 cggcaaggcg tagaaggccc accacttgtc gccatgcttg accaccgtgg cgtcgatggc
     1981 cggaacatcc atgatctgct gggccggctc ccaaacatcg gggaagcgta cgcaacgata
     2041 cagggtcagg ccgccggctt tgtaaccctc cggcagcatg tagagctcgc caccgtcttc
     2101 gatcagcgac ggataggaca ggtggattgg cgcggtcagg gccacgccgt gcccggtcag
     2161 cgcgttatcg gcgctgtact gatagaaatg gatttcaccg cgacgaacgc gatagtcgaa
     2221 ggcttcgacg aagaccgtca gcaccccgtc gcgttcgatt ccgaatggat cggcgacgta
     2281 ctggaacggc ccggagttgg gcagccaggt cacttccgac gcgtcaacac cggctgtcgc
     2341 cgtcacgctg gaaagcgaac gcctgacaaa accgacacgc cagatatcgt acattgcgac
     2401 ccccgccgga ccgcctcacg caggtctcgg acgtaggacg ccgccggatg cggcttgcct
     2461 cacaaaaatc gacgcggcat gaagtctgca ggcgttcgat ccggttcggc gtaacgccgt
     2521 cgaccgagcc gaaacgagaa ggactcccgt ttttgaccgc gcaagccgcg catccgctga
     2581 tcggcgccta tttcgagcat cgcgccgccc tgggtcggtt cctgcaggcg cggctcggtg
     2641 cgtctggtga tgtcgaggat ttgctgcagg atctctatct gaaggtcgcc gcgctcgatc
     2701 cggcgatcga ggtgcgagac gctcgcgcct tcctgtaccg cctcgcatca aatcagatga
     2761 tggatcgctg gcggtccggt cagcgatcgg tcgctcgcga cgctgcatgg cggctggcta
     2821 atcacgctgt cggcgagaca gaggacctga acgacgcgcc gtcggccgag gccgtcgtca
     2881 tcggacgcca gaaactcgca gccctgaccg cagcggtgtc ccgccttcca gacaagaccc
     2941 gcacggtgtt cagcctgcac aagttcgagg gcttcagcta cgccgaggtg gctggccggc
     3001 taggcatttc gcgcagttca gtcgagaagc acatgatgga cgcgctccgg gtgcttagcg
     3061 cgagaataag atcgtgattt tcgcggagac gttgaaaact tggcacgaca acggaggtga
     3121 caggcggtcg gtgacgtcta ttggtcatac accctctgac acgacgagaa tccggcttcc
     3181 caacgcatga ccacttcgca accggacatc gaagaacagg cgctggagtg gttcgtccgc
     3241 ttgagcgacg tcgatgcacc cgcgtctgca tggatgtcgt ttcaggactg gctggaggcc
     3301 gacgaggctc acggggccgc ctatgaggct gtcgaacgaa cctgggtcgt gctcgacggc
     3361 gtggttccaa ccgtcgccgt tctgcctgta gcagcgaacg accagagctc ccccgcccgc
     3421 gcgtcgaacg cgcggcgtcg acgccctgtt tggctgctgc catcattcgc cgcagcggcc
     3481 gctgcggcga tggtcatcgg catctggcgc cagatcagtg ccgtcgcgcc cgtcgagagc
     3541 tatcacacgg accttgagcc aaggaccgtc gtcctgtccg atggctcccg tgtggtcatg
     3601 aaccgccact ccgacctgag cgtacggata agacgcgaca accggaccgt gaccctcgct
     3661 gacggtgagg cggccttcga cgtcgctcat aatcccgacc ggccgtttgt gatcactgcg
     3721 ggcgaccata gcgtccgcgt tctcggaacc gcctttaacg tcctcaatc
//
LOCUS       DASVTM010000101         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_435716,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000101 DASVTM010000000
VERSION     DASVTM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435716"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3169)
                     /locus_tag="VF633_02880"
     CDS             complement(<1..3169)
                     /locus_tag="VF633_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8470034.1"
                     /translation="MSASLLASFVVTAAVSTWIDLRRQATLETNRLTQTARVIGSLAS
                     DAVVKGDPAATFTAIRSVSQMPGISYARISSERFGLLAETGAGARLISEATVEGADQK
                     LSVWSILSTGSIQVSAPVMSDGAPVGSITLFGRTPELRGRVLSAIWATLAGALIAVLA
                     GLIVALRMAHKISSPIVALAGLVRRVHDTRDFSTSVDIHADGEVADLVAGIDTMLSGI
                     RERDTRIAAQVEGLESEVAARTGELIVARDAAEQANAAKSDFLAVMSHEIRTPLNGIL
                     ALSDMLATSDLPQRQQRYADVISKSGRSLLSIINDILDFSKVEAGKMDMETVEVDLAE
                     AAEDVASLFSEKAASKGLDLAVFVDPRLPIVIGDPTRLRQVIGNLVNNAIKFTDVGGV
                     LIVIDREPADMERVRIAVQDTGPGIPADKLPNLFEAFTQADQSTTRTHGGTGLGLAIC
                     DRLVRSMGGVWALSSTIGKGSTFAFSAPLKTASVPVKAPGLEPNWSVSVRDLSAQTRQ
                     AVLLYLAAWGLTATANEHGPCIVGAGQAAPGLGAVICDSEDAAAAVLRSAPAACVMVK
                     PLRLSELRDVLGQMQRGEQPRLTHQTPRVQAGTTFPGVRVLVVDDSEVNREVATEALS
                     RLGVETFSACDGQQAVERLRTQSFDMVLMDGSMPVLDGFEATRAIRSEEAQTQRPRAV
                     IVALTAHVVGSGAEAWKASGMDGLIHKPFTLDDLARELTRFCGDRAVAGSTVSVPTPA
                     ATEDEAEADLFDPSIRADLFSMGRNGRADFVDRVHSLYATNAPLRLTEIVDSATANDN
                     DSLARAAHALKSMSLSLGASAVAREASRIETAARSGAPAEVEAIQSLVSRTLAAMAEP
                     ADPPAIKTTAEEDLAVAIERGELHLVYQPLVERKGHLSGKVEALIRWTHPQHGRLSPD
                     DFIPRLEAEGGIERLTDFVMTRAMQDLADRPDIRVSINASASEFQARGFAQRVAAAAS
                     ALSFPPERLEVEVTETAMLDVEAARRTIEELRTAGFDVALDDFGAGYTSLHALRELKF
                     TTLKVDGSFVNQCTDDTASAAIIHAV"
     gene            3336..3788
                     /locus_tag="VF633_02885"
     CDS             3336..3788
                     /locus_tag="VF633_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470035.1"
                     /translation="MSGFFYVLDDEARLLFVDDDPILREFAQVNLASAAAEIDVAADG
                     VEALEALSRCKYDLLLIDLDMPRMDGFELLAHLRADPATAHLPVVVETGREDVEAIDR
                     AFRAGATAFVTKPLNWRLLAYQLRFTLRAARAEAALRDRYRSATAVAA"
     gene            3785..>4124
                     /locus_tag="VF633_02890"
     CDS             3785..>4124
                     /locus_tag="VF633_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX8470036.1"
                     /translation="MTSLLQMLERGRLWLRSFRHSEQGNIAVIFALAAPALFLIGVGA
                     IDLGRVQSNRVKLQDIADAAALAGANELGLAINDTVAIERAKVFVQGHVLEWKEGPDI
                     SPEISVISKDG"
BASE COUNT          722 a         1293 c         1331 g          778 t
ORIGIN      
        1 tcacggcgtg gatgatcgcg gctgacgcgg tgtcgtcggt gcattggttc acaaagctgc
       61 cgtccacctt cagcgtcgtg aacttcagtt cgcgaagggc gtggaggctg gtgtagcccg
      121 cgccgaaatc atctagcgcc acgtcgaaac cggcggtgcg cagttcttcg atggttcgcc
      181 tcgcggcttc gacgtcgagc atcgcggtct ccgtcacttc gacttccaga cgctcaggag
      241 gaaagctcag ggcgctggcc gctgcggcga cccgttgagc aaagccgcgc gcctggaact
      301 cggacgccga ggcgttgatc gatacccgga tgtccgggcg gtcggcgagg tcctgcatgg
      361 cgcgcgtcat cacgaagtcg gtcaaccgtt cgatgccgcc ttcggcttcc agccggggaa
      421 tgaagtcatc gggactcagg cggccgtgct gcggatgggt ccagcggatc aacgcctcga
      481 ccttgcccga cagatgccct tttcgttcta caagcggttg gtagacgaga tgaagttccc
      541 ctcgctcgat ggcgaccgcg aggtcctcct cggcggtcgt ctttatagcc ggcggatcag
      601 ccggctccgc catagcggcc agggtgcgcg agacaagaga ctgaatagct tccacttcgg
      661 cgggcgcgcc ggagcgcgcc gcagtctcga ttcgggaagc ttcccgtgcc accgccgacg
      721 cgccgaggct caggctcatg gacttcagcg cgtgagcagc gcgggccaga ctgtcgttgt
      781 cattggcggt ggcgctgtcg acgatctcgg ttaaccgcag aggcgcgttc gtcgcataaa
      841 gggagtggac ccgatccacg aagtccgcgc gtccgttgcg acccatagag aacagatcgg
      901 cgcggattga gggatcaaac aagtcggcct cggcctcgtc ttcggtagca gccggggtcg
      961 ggacagaaac ggtggatccg gcgacggccc gatcgccgca gaagcgtgtc agctccctgg
     1021 ccaggtcgtc cagtgtgaaa ggtttgtgga tcaagccgtc catgcctgac gctttccagg
     1081 cctcggcgcc ggaaccgacg acgtgcgctg tcaaggcgac gatcacggcg cggggtcttt
     1141 gtgtctgggc ctcctcggag cggatagcgc gcgtcgcctc gaagccgtca agaacgggca
     1201 tcgagccgtc cataagaacc atgtcgaagc tctgtgttcg aagacgttcc actgcctgct
     1261 gtccgtcgca cgcggaaaag gtctctacgc ctagacgtga cagggcctcg gttgcgacct
     1321 cccggttaac ttcggagtcg tcaacgacga gaacccgaac gccgggaaaa gtcgtgccgg
     1381 cctgaacgcg cggcgtctga tgtgtgagcc ggggttgttc gccccgctgc atctgcccaa
     1441 ggacgtcgcg caattccgac agccggagcg gcttcaccat cacacaggcc gcgggagcgg
     1501 agcgaagcac cgctgccgcc gcgtcttcgc tgtcacagat cacggctccc aaacccggcg
     1561 cggcctggcc tgctcccacg atgcacgggc catgctcgtt tgctgtagcc gtgagtcccc
     1621 aggcggcgag atagaggagg actgcctgtc gggtctgcgc cgacaggtcg cggacgctca
     1681 cactccagtt cggctcaagg ccgggcgcct ttaccgggac tgaagccgtc ttcagcgggg
     1741 cggagaacgc aaatgtcgat cccttgccga tggtgctgga cagcgcccag acgccgccca
     1801 tcgaacgaac aagccgatcg cagatcgcga gcccaaggcc ggtgccgccg tgcgtgcggg
     1861 tggtgctctg atcagcctgc gtgaatgctt cgaacaggtt cggcagttta tcggcaggga
     1921 tgcctgggcc ggtgtcctga accgcgatgc gaacccgttc catgtccgcc ggttcccgat
     1981 cgatgacgat cagcacgccc ccgacatccg tgaacttgat ggcgttgttc accaggttcc
     2041 cgatgacctg ccgcagacga gtggggtcgc cgatgacgat gggcaggcgt ggatccacga
     2101 atacggcaag atccagccct ttgctcgcag ccttctctga aaacaggctg gcgacgtcct
     2161 cggccgcctc cgcgagatcg acctccacgg tctccatatc catcttgccg gcctcgacct
     2221 tcgagaaatc gaggatatcg ttgatgatgc tgagtagcga cctgcccgat tttgagatga
     2281 cgtccgcgta gcgctgctgg cgctggggca agtcggaggt cgcgagcatg tcactgagcg
     2341 ctagaatgcc gttgaggggc gtgcgaattt catggctcat gaccgccaga aagtccgatt
     2401 tggcggcatt tgcctgttca gcggcgtccc gtgcgacgat gagttccccc gtgcgtgctg
     2461 caacctcgct ttcgagccct tcgacctggg ccgcgatacg cgtgtcgcgt tcgcggatgc
     2521 cgctaagcat ggtgtcgatg cccgccacca gatcggcgac ctctccgtcg gcgtggatgt
     2581 caacggaggt cgagaagtcc ctggtgtcat ggacccgcct gaccagaccc gccaaggcga
     2641 cgatcggcga actgatttta tgcgccattc tcagggctac gatcaggcct gcgaggacgg
     2701 cgatcagggc tcctgccaag gtcgcccaga tcgcggacag cactcggcct cgcaattcgg
     2761 gtgtccggcc gaacagcgtg atcgagccga ccggcgcgcc atcgctcatg accggcgctg
     2821 agacctgaat cgatcccgtg ctcaggatcg accagaccga gagcttctgg tcggcgcctt
     2881 cgaccgtcgc ctcggagatg agacgagccc ccgctcccgt ttcagcaagg aggccgaacc
     2941 gctcggagct gatgcgggca tagctgatgc cgggcatctg gctgaccgat cgaatggctg
     3001 tgaaggtcgc cgcgggatcg ccctttacaa cagcgtcgga agccagtgat ccaatcaccc
     3061 gggccgtctg ggttagacgg ttcgtttcca gcgtcgcctg ccggcgcagg tcgatccagg
     3121 ttgaaactgc cgccgtcacg acaaacgacg ccagcagcga ggccgacacc aggccggcca
     3181 atttccgagc gatgccaccc ctggttctaa gtcggctacc gtacacgttc gagctccagg
     3241 ccgctgtagt tcctaggtta tacgtgatgt taatgacctg acccttaagt cctgcgttcg
     3301 gagtcagaga ggcgccaagc ctccggggtg ttctgatgag tggctttttc tatgttctcg
     3361 atgacgaggc tcgtcttctg ttcgtggacg atgatcccat cctgcgggaa ttcgcccagg
     3421 tgaatctggc ttccgccgcc gccgagatcg acgtggcggc ggatggcgtt gaagccctcg
     3481 aggcgctctc gcgctgtaaa tatgatctgc ttctgatcga cctcgacatg ccgcgcatgg
     3541 acggcttcga actgctggcc catctccgcg cggaccctgc gacagcccat ttgccggtcg
     3601 tggtggaaac gggacgtgag gacgtggagg ccatcgatcg ggccttccgg gccggggcga
     3661 cggccttcgt caccaagccg ttgaattggc gtctgttggc ctaccaactc cgcttcacgc
     3721 tgcgggccgc ccgcgccgaa gcggccctga gagaccgcta cagatccgca actgctgttg
     3781 cggcgtgacc agcctgttgc agatgctgga gcgcgggcgc ctgtggctcc gcagcttccg
     3841 gcatagcgag caaggcaata tcgcggtgat attcgccctt gccgcgcctg ccctgtttct
     3901 gatcggggta ggggcaatcg atcttggacg cgtgcagtcg aaccgggtca agcttcagga
     3961 catcgccgac gccgctgcgt tggccggcgc gaatgagctt ggactggcta tcaacgacac
     4021 agtcgccatc gaacgggcca aggttttcgt tcaggggcac gtcctggaat ggaaagaggg
     4081 ccccgacatt tcgccggaaa tctcggtcat ttcaaaggat ggcc
//
LOCUS       DASVTM010000102         8344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_276598,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000102 DASVTM010000000
VERSION     DASVTM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276598"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VF633_02895"
     CDS             <1..555
                     /locus_tag="VF633_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX8470037.1"
                     /translation="HALRAYTPSDQRGLKAASESFRVNPAFNTAEAIQALGTGEALVS
                     VLDEKGAPTITARTLIRPPGSRLGPATDAERAAVMAASPVAGVYDKAIDRESAEEVLA
                     ARHAALDKLADDEATAKAEAKAPRAPRAAPAPRAPAPRAPAAPRKSTRQTPLEALTKS
                     VLRTAGSTITRELLRGVLGGFKRR"
     gene            complement(603..2765)
                     /locus_tag="VF633_02900"
     CDS             complement(603..2765)
                     /locus_tag="VF633_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8470038.1"
                     /translation="MRPVLLSFAMSLMASTALAQSAPPHMPTDLSPAGVRAADEHLAL
                     EEVNGTEAMAFVASENEKSLKSLTGDPRYEGFRQQAFDILSATDRIAAPSFLGEGIGN
                     FWQDATNAKGLWRRTSLDSYRSATPQWETLIDLDALSKTEGKDWVWKGGDCLPPDETR
                     CLISLSDGGKDAVVVREFDITTKTFVDGGFSLPEGKHRIEWLDQDTLLVATDFGPDTL
                     TESGYPFIVKGLRRGQTLEQAVEVFRGAPTDGGYGVSPRAFRNGDGELQAVIVTRPLD
                     TFKAETWLIDGAKTIKLGLPEKVTIHGMIDNRLVFTPDQDWVRRGKSRTAGSLLAVSL
                     NVLRPPVGAGDIFVSTEADTVIVPTARQSIEDVAVLDEKIVATVYDNVTGHVVAFNNH
                     GTQPWTRTVLPAADNAAVHLGSFSRKAGRIFYTFEGFLTPATLALSDLVANTANVIRS
                     APARFDASRDVVEQFEATSSDGTKIPYFVVRPKDAPMDGSTPTIMFGYGGFQVAFPPA
                     YKPEMGKLWLENGGAFVIANIRGGGEFGPSWHDAALREHRQLAFDDFAAVARDLKDRK
                     ITSPRRLGIYGRSNGGVLTSVSITQHPELFNAAVIESPLIDMLRYPDLPAGASWIGEY
                     GDPRVPADAAFIARYSGYQNLRAGARYPRVYITTNTRDDRVHPGHARKFAARLGDMGY
                     DHLYYEETSGGHSNDADPVANARRWARHYTYLSQQLMD"
     gene            complement(2781..4988)
                     /locus_tag="VF633_02905"
     CDS             complement(2781..4988)
                     /locus_tag="VF633_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8470039.1"
                     /translation="MMRSLASAAALLVATGMTTAAMAQTTALPSASAPARQAARPANP
                     PIAEGGFATSDATDPYLWLEEVDGARAMEWVKAHNERSLGILQSDPRYVPLHQQALAI
                     VQSKDRIPAPGFNHDGSITNFWQDAEHVRGVWRKTTLESYRTDTPQWETVLDIDVLAT
                     AEGKNWVYKGSSCLPPDERLCLVSLSDGGKDAVVVREFDSVSKTFVDGGFNLPESKGG
                     ADWIDADTLLIARDFGPGTMTTSGYPMIVKRLKRGQTVDQAETIFTGQPSDVSVSGSA
                     LRDADGVIKAVLISRGVSFYESETYRLNADGTTTKLELPAKSDIDALVQGQLIVTTKQ
                     DWTAPSGQEFKTGDVIAWNLDAWLADPKAEAKLVIRPTERQAIEGITSTRNQLVVALF
                     DNVRGSVQVYDPKDWSHTRLDLPENVTVGVGSASETDDKIFVSVAGYLNPSSLYLADA
                     AAGSAAVVKSLPAKFDATGMTVDQFEATSADGTRIPYFVVHKDAIPMDGSNPTLLYGY
                     GGFQSSLLPGYSATVGKLWLERGGVYVVANTRGGGEFGPKWHDAALQENRHKAHEDFQ
                     AVALDLEARNITSQPKLGIMGGSQGGLFMGAMLTQRPDLINAAVIQVPLFDMLRFNKL
                     LAGASWQGEYGNPDVPREREWIQAYSPYQNLRAGMNYPEVFIHTSTKDDRVHPGHARK
                     AAARLEELGYPVLFYENTDGGHAAGANLLETARRLALEYTYLTRRLMDQPAQQ"
     gene            complement(5119..5922)
                     /locus_tag="VF633_02910"
     CDS             complement(5119..5922)
                     /locus_tag="VF633_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX8470040.1"
                     /translation="MTASSARPHKHHGLRLRPRLRYLYHGDSLTAVRFRLAVIVIDLA
                     IIAFFIAAPVLKEHGRTFYVLDYTIAALLLIDLAARITAYSDWKDWFKRPINWVDIVV
                     LATLLVPAWAFNLGFLRILRLWTLLNSTMFWRTIGHRFDDTRVEEITRALSALVTFVF
                     VVTGFVYTVFRGRHDGITGYLDALYFTVATLTTTGFGDITLPGAWGRIISITVMLVGI
                     TLFLRLAQTLIRPHKVRYPCEKCGLQKHDPDAVHCKACGNLLCILDDGG"
     gene            6005..6862
                     /gene="dapF"
                     /locus_tag="VF633_02915"
     CDS             6005..6862
                     /gene="dapF"
                     /locus_tag="VF633_02915"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX8470041.1"
                     /translation="MTRPFVKMNGAGNDFIVVNALERPFTPTVDQVRALGDRQSGEGF
                     DQLISIEPSKTGDAFMRVWNNDGSVVETCGNALRCVGWMLMQASGADRVTIDTAGGPA
                     VAMRANDGLGEYRITVDMGAPRLDWTQIPLSEEMDTRGIELQVGPIDAPLLHTPGAVS
                     MGNPHVVFFMDHTPDDAFVRGSGSLIEHHPLFPEAVNVGFAHVEARDRIRFRVWERGA
                     GLTKACGTGACAALVATARRGLTDRKATVVMDGGEVVIDWAPDENGASGHVFMTGPVE
                     VERTGGLSI"
     gene            complement(6880..7218)
                     /locus_tag="VF633_02920"
     CDS             complement(6880..7218)
                     /locus_tag="VF633_02920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8470042.1"
                     /translation="MALLRNPDERRTESRLPANARGVIVAPSLEMACLIVDLSDGGMR
                     VRTDRSVSLPSVVTIVDIAAGTACEADVAWRKGHEAGLKCRVRATALRGLVPARFAQA
                     REAWLRAGGR"
     gene            complement(7275..7595)
                     /locus_tag="VF633_02925"
     CDS             complement(7275..7595)
                     /locus_tag="VF633_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8470043.1"
                     /translation="MAKVTYIQHDGGEQTVEVKNGLSVMEGAIRNNVPGIDADCGGAC
                     ACATCHVYVDPDWMQEAGKPSAMEESMLDFADNVEPNSRLSCQIRVSDTLDGLVVRLP
                     ESQH"
     gene            7911..8285
                     /locus_tag="VF633_02930"
     CDS             7911..8285
                     /locus_tag="VF633_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX8470044.1"
                     /translation="MIAADSSVLIDAMKGLGGADIESLLTALKADQLRLPPPVVTEVL
                     SFPGSAARLQKLPQFKQLPILEQFWERAAVSRRKLLERGLKAKLGDALIAQCCIDADV
                     ALLTRDTDFRHFANHCGLKLAV"
BASE COUNT         1463 a         2555 c         2859 g         1467 t
ORIGIN      
        1 cacgcgctgc gcgcctatac gccgtcggac cagcgcgggc tgaaggcggc gtcggagagc
       61 ttccgcgtca acccggcctt caataccgcc gaggccattc aggcgctggg gaccggcgag
      121 gcgctggtgt cggtactgga cgagaagggg gcgccgacga tcaccgcccg taccctgatc
      181 cgtcccccgg gcagccgtct gggtcccgcg acggacgccg agcgcgctgc agtcatggcg
      241 gccagtcccg tcgccggggt ctatgacaag gcgatcgacc gcgagtccgc cgaggaggtt
      301 ctggccgctc ggcacgccgc tttggacaag ctcgccgatg acgaagcgac tgcgaaggcc
      361 gaggcgaaag cgccacgagc gcccagggca gctccggctc cacgtgcccc tgcccctcgc
      421 gcccctgccg caccgcgcaa gtccacacgt cagacaccgc tggaagccct gaccaagtcg
      481 gtgctgcgca ccgcgggatc gaccatcact cgcgaactgc tgcgaggcgt tttgggtggg
      541 ttcaagcggc ggtagccctt ggtcccgctc atcgctgcga gcgatgagcg ggaaggatgc
      601 gcctagtcca tcaactgctg ggacagatag gtgtagtgcc tcgcccagcg gcgggcgttc
      661 gcgaccggat cggcgtcgtt ggagtgaccg cccgaggtct cttcgtaata gaggtggtcg
      721 tagcccatgt cgccgagacg ggccgcgaat ttgcgcgcat ggccgggatg gacgcggtca
      781 tcgcgggtat tcgtcgtgat gtagacgcgg gggtaccggg ctccggcgcg caggttctga
      841 tagccggaat agcgggcgat gaaggcggcg tctgcgggca cgcgcgggtc gccgtactcg
      901 ccgatccatg aggccccggc gggcaggtcc ggatagcgca gcatgtcgat cagcgggctc
      961 tcgatcaccg cggcgttgaa cagttcgggg tgttgggtga tcgagaccga ggtcagtacg
     1021 cccccgttgg accggccata aatgccgagg cgacgcggcg aggtgatctt gcgatccttg
     1081 aggtcacggg ccacggcggc gaagtcgtcg aaggccagct ggcggtgttc gcgcagtgcc
     1141 gcatcgtgcc aggacggccc gaactcgccg ccgccgcgga tgttggcgat gacgaaggcc
     1201 ccgccgttct cgagccacag cttgcccatc tcgggcttgt aggcgggcgg gaaggcgacc
     1261 tgaaagccgc cgtagccgaa catgatggtc ggggtcgatc cgtccatcgg cgcgtccttg
     1321 gggcggacga cgaaataggg aatcttcgtg ccgtcgctcg aggtcgcctc gaactgttcg
     1381 acgacatccc tcgacgcatc gaaccgcgcc ggggcgctgc ggatcacgtt ggcggtgttg
     1441 gcgaccagat cggacagggc gagcgtcgcc ggggtcagga atccctcgaa cgtatagaag
     1501 atacggccgg cctttcggct gaaggagccc agatggacgg ccgcgttgtc cgcggcgggc
     1561 agaaccgtcc gggtccaggg ctgcgtgccg tggttgttga acgccaccac gtggcccgtg
     1621 acattgtcat agactgtggc gacgatcttc tcgtccagca cggccacgtc ctcgatggac
     1681 tggcgggcgg tcggcacgat gacggtgtcg gcctcggtcg agacgaagat atcacctgca
     1741 ccgaccggag ggcgcaggac gttcaggcta acggccagca gggagccggc ggtgcgagac
     1801 tttccgcgac ggacccagtc ctgatcgggc gtgaacacca gccggttatc gatcatgccg
     1861 tgaatggtga ccttttccgg gaggcccagc ttgatggtct tcgccccgtc gatcagccac
     1921 gtctcggctt tgaaggtgtc gagcgggcgg gtgacgatga cggcctgaag ctcgccgtcg
     1981 ccattccgga aggcgcgcgg gctgacgccg tagccaccgt cggtcggagc gccacggaaa
     2041 acctcgaccg cctgttccag cgtctggcct cgtctcagcc ccttgacgat gaaggggtag
     2101 ccgctctcgg tcagcgtatc gggaccgaag tcggtggcga ccagcagcgt gtcctgatcc
     2161 agccattcga tgcggtgctt gccctccggc aggctgaagc cgccatcaac gaaagttttg
     2221 gtcgtgatgt cgaactcacg cacgacgaca gcgtccttgc cgccgtccga caggctgatc
     2281 aggcagcggg tctcatcggg cggcaggcag tcgccgccct tccagaccca gtccttgcct
     2341 tcggtcttcg acagggcgtc gaggtcgatc agggtctccc actgcggcgt ggccgagcgg
     2401 taggaatcca gcgacgtgcg ccgccagagg cctttggcgt tggtcgcatc ctgccagaaa
     2461 ttgccgatac cctcgccgag gaagctgggc gcggcgatgc ggtcggtggc tgacaggatg
     2521 tcgaacgcct gctggcggaa gccttcatag cgcggatccc cggtcaggga cttgaggctc
     2581 ttctcgttct ccgaggcgac gaaggccatg gcctcggtcc cgttgacctc ctccagcgcc
     2641 agatgctcgt ccgccgcccg cacccccgcc ggcgacaggt cggtcggcat gtggggcggg
     2701 gcggactggg cgagagcggt ggacgccatc agggacatgg cgaaggacag caggacaggg
     2761 cgcatcgtca gactttcaag ttactgctgc gccggctgat ccatcagccg ccgggtcagg
     2821 taggtgtatt ccaaggccag acggcgggcg gtttccagca gattcgcacc ggccgcgtgt
     2881 ccgccgtcgg tgttctcata gaacagcacc ggatagccga gctcttccag acgggctgcg
     2941 gccttgcggg cgtggcccgg gtggacgcga tcgtccttgg tcgaggtatg gatgaagacc
     3001 tcggggtagt tcatcccggc gcgcaggttc tggtacggcg aataggcctg aatccactcg
     3061 cgttcgcgcg gcacgtcggg attgccgtac tcgccctgcc acgaggcgcc ggccagcagc
     3121 ttgttgaagc gcagcatgtc gaacaggggc acctgaatga cggcggcgtt gatcaggtcg
     3181 gggcgctggg tcagcatcgc acccatgaac agcccgcctt gcgagccgcc catgatgccg
     3241 agcttgggct gggaggtaat gttgcgggcc tcgaggtcga gggcgacagc ctggaagtct
     3301 tcgtgcgcct tgtggcggtt ttcctgcagg gccgcgtcgt gccatttcgg accgaactcg
     3361 ccgccgccgc gtgtgttggc gacgacatag acgccgccac gctcgagcca cagcttgccg
     3421 accgtggccg aatagccggg caggagcgag ctctggaaac caccgtagcc gtacagcagc
     3481 gttgggttgg agccgtccat cgggatggcg tccttgtgga ccacgaaata agggatcctg
     3541 gtgccgtcgg cagaggtggc ttcgaactgg tcgaccgtca tgcctgtggc gtcgaacttg
     3601 gcgggcaagg atttgaccac ggcggccgag cctgcggcgg cgtcggccag atagaggctg
     3661 gaggggttca ggtagcccgc gacgctgacg aagatcttgt cgtccgtttc gctggccgag
     3721 ccgacgccga ccgtgacgtt ctcaggcagg tcgagccggg tgtggctcca gtccttcgga
     3781 tcatagacct gaaccgagcc ccggacgttg tcgaacaggg cgacgaccag ctggttgcgg
     3841 gtcgatgtga tgccttcgat cgcctgacgc tcggtcgggc ggatgaccag tttggcctcg
     3901 gccttgggat cggcgagcca ggcgtcgagg ttccatgcga tcacgtcgcc ggtcttgaac
     3961 tcttgtcccg aaggcgcggt ccagtcctgc ttggtcgtga cgatcagctg gccctggacc
     4021 agggcgtcga tgtcagactt ggcgggaagc tccagcttgg tcgtcgtgcc gtcggcgttc
     4081 agacggtagg tctcgctctc gtagaagctg acgccacggc tgatcaggac cgccttgatg
     4141 accccgtccg cgtcgcgcag ggccgaaccg ctgaccgaga cgtccgaggg ttggccggtg
     4201 aagatcgtct cggcctgatc gacggtctga ccacgcttca gccgtttgac gatcatcggg
     4261 taacccgaag tcgtcatcgt gccgggaccg aagtcgcgtg cgatcaacag ggtgtcggcg
     4321 tcgatccagt ccgcgccgcc ctttgattcc ggcaggttga aaccgccgtc cacgaaggtc
     4381 ttcgagacgc tgtcgaactc gcgcacgacc acggcgtcct tgccgccgtc ggacagggaa
     4441 acgaggcaca gacgctcatc cggcggcagg caggatgaac ccttgtaaac ccagttcttg
     4501 ccctcggcgg tcgcgaggac gtcgatgtcg agcacggtct cccactgggg agtgtcagtg
     4561 cggtagctct ccagcgtcgt cttgcgccag acgccgcgta catgttcggc atcctgccag
     4621 aagttggtga tcgagccgtc gtggttgaag ccgggcgccg ggattcggtc cttagactga
     4681 acgatcgcca gggcctgctg atgcagcggc acgtagcgcg ggtcgctctg caggatgccg
     4741 aggctgcgtt cgttgtgggc cttgacccac tccatggccc gggcgccgtc gacttcctcg
     4801 agccacagat aggggtcggt cgcgtccgag gtggcgaaac cgccttcggc gatcggggga
     4861 tttgcgggac gtgcggcttg gcgagcggga gcggacgcgg aaggcaaggc ggtcgtttga
     4921 gccatggcgg cggttgtcat tccggttgcg acgagaaggg cagcggccga ggccagcgaa
     4981 cgcatcatat gggggctcct tgaaggttgg ggcaccttag cggcgaaggc ttctgcgaca
     5041 agacaaacga ccttacggat ttgtcatgcg cgacatttgg tagccgtgca gacgccctcg
     5101 cttgataacc ggcttcggtc agccgccgtc gtccagaatg caaagcagat tcccgcaggc
     5161 cttgcagtgg acggcgtcgg ggtcgtgttt ctgcagtccg cacttctcgc acgggtagcg
     5221 aaccttgtgc ggtcggatca aggtttgggc caatcgcagg aaaagggtga tgccgaccag
     5281 catgacggtg atcgagatga tccgccccca ggcgcccggc agggtgatgt caccgaaacc
     5341 cgtggtcgta agcgtcgcca cggtgaaata cagggcgtcg aggtagccgg tgattccgtc
     5401 gtgtcggccc cgaaagacgg tgtagacgaa gccggtgacg acgaagacga aggtgacgag
     5461 ggccgacagg gcgcgtgtaa tctcctccac ccgggtatcg tcgaaccggt gtccgatcgt
     5521 gcgccagaac atggtcgagt tcagcaaggt ccacaggcgc aggatcctga ggaagccgag
     5581 attgaacgcc caggcgggca ccagcagtgt cgccagcacg acgatatcga cccagttgat
     5641 cggacgcttg aaccagtcct tccagtccga ataggccgtg atccgcgccg ccaggtcgat
     5701 gagaagcagg gcagcgatcg tgtagtccag cacatagaag gtgcgtccat gctccttcag
     5761 caccggggcg gcgatgaaga aggcgatgat ggccagatcg atgacgatca cggcgagcct
     5821 gaaccgcacc gccgtcaggc tgtcgccatg atacagatat cgcagtcgcg gcctaagccg
     5881 caggccgtga tgtttgtggg ggcgggcgga agaagcggtc atggaccagc gataacgcgc
     5941 gtctcgccag aacgaaacgc ggaagcttcg cggcgcgccc acgacaccct atatatgcaa
     6001 cgacatgacc cgcccctttg tcaaaatgaa cggagccggc aacgacttca tcgtcgtcaa
     6061 tgcgctcgaa cgaccgttca caccgacggt cgaccaggtg cgcgcgctgg gcgaccggca
     6121 aagcggcgag gggttcgacc agctcatctc gatcgagccg tcaaagaccg gcgacgcctt
     6181 catgcgggtc tggaacaacg acgggtcagt ggtcgagacc tgcggcaatg cgttgcgatg
     6241 cgtcggctgg atgctgatgc aggcctcggg cgcggaccgg gtgaccatcg acacggcggg
     6301 cgggccagca gtggcgatgc gcgccaatga tgggttgggc gagtaccgga tcaccgtcga
     6361 catgggcgca ccgcgtctcg actggaccca gatcccgcta tctgaggaga tggatacgcg
     6421 cggtatcgag ctgcaggtcg gaccaatcga tgcgcctttg ctgcacacgc ccggcgcggt
     6481 ctcgatgggt aacccgcacg tggtcttctt catggaccac acgcccgacg atgccttcgt
     6541 gcgtggttca gggtcgctga tcgagcacca ccccctgttt cccgaggcgg tcaatgtcgg
     6601 tttcgcccac gtggaggccc gcgatcgcat tcgtttccgt gtctgggagc gtggcgccgg
     6661 tttgaccaag gcctgcggca ccggcgcctg cgcggcgctg gtcgcaacgg cgcggcgcgg
     6721 gttgactgat cgcaaggcga cggtggtgat ggacggcggc gaagtggtga tcgactgggc
     6781 cccggacgag aatggggctt cgggccacgt cttcatgacc ggtcctgtcg aggtcgaacg
     6841 gaccggcggg ctgtcgatct aggcggagcg acccgtccct cagcggccgc ctgcgcgcag
     6901 ccatgcctcg cgcgcctgag cgaagcgggc gggtaccaga ccacgcaggg cggtggcgcg
     6961 gacgcggcat ttcagtccgg cctcatgccc cttgcgccaa gccacatcgg cttcgcaggc
     7021 ggttccggcg gcaatgtcga cgatcgtcac gacggacggc agggaaaccg atcggtcggt
     7081 acgaacccgc atgccgccgt cggacaggtc gacgatcaaa caagccatct ccaaagacgg
     7141 agcaacgatc actccccgcg cattggcggg caggcgggat tcggttcggc gttcgtctgg
     7201 attacgcaac agggccatgg gtcagcatac cgcctgacgt cgccgttgta tgcccgaacc
     7261 gccgatcgac ctgatcagtg ctggctttcc ggcaggcgga cgacaagtcc gtccaaggtg
     7321 tcggacaccc ggatctggca cgacaggcgt gagttcggct cgacgttgtc ggcgaagtcg
     7381 agcatcgact cctccatggc gctcggcttg ccggcctcct gcatccagtc gggatcgaca
     7441 tagacgtggc aggtcgcaca ggcgcaggcg ccgccgcagt cggcatcgat ccccggcacg
     7501 ttgttccgga tcgcgccttc catgaccgac aggccgttct tgacctccac ggtctgctcg
     7561 ccaccgtcgt gctggatgta ggtaactttg gccatgggcg ggtcttagca agggtggcga
     7621 tagaggcaag tgctgtaagt tgatgccagc atcgttttat ggtaacatac cgcaatgaca
     7681 gacatgcgga aaatcaccgt cttcctaccc gcggatttgc tcgagagctg tcagcaaata
     7741 acaggcaagg gggtttcgga aaccattagg accgcccttg ccgatctcgt ccaccacgcg
     7801 gctcgagaag agcttttggc ggctatgggg catctcgatc tggaggcgga tggtttgaca
     7861 ctgcaagatc tgagggatga gagcccaagc ctgcccgcgc atcacgcagc atgatcgccg
     7921 ccgacagcag cgttctgatc gatgcaatga aggggctggg cggagccgat attgaaagct
     7981 tgctgacagc tttgaaggcg gaccagcttc gcttgcctcc tccggtcgtc accgaggttc
     8041 tttcgtttcc aggctccgca gcccgcctcc aaaaactacc tcagttcaag caactgccga
     8101 tcttggagca gttctgggag cgtgctgcgg tatcgcgcag aaagttgttg gagcgtggtt
     8161 tgaaagccaa gctcggcgat gcattgatag cccaatgctg catcgacgcc gacgttgcgc
     8221 tgctcacgcg agacacagac tttcgccact tcgcaaacca ctgcggactg aagttagcgg
     8281 tctaaagttg ctcgctcagg ctgcgacata gaccgacgcg acaaagcccg ggtgtccgag
     8341 cccg
//
LOCUS       DASVTM010000103        10399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_169419,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000103 DASVTM010000000
VERSION     DASVTM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169419"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(635..1414)
                     /gene="pgeF"
                     /locus_tag="VF633_02935"
     CDS             complement(635..1414)
                     /gene="pgeF"
                     /locus_tag="VF633_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX8470045.1"
                     /translation="MSDLPPPIVHPLLTKAGVRHGFFTRQGGVSTGIFEGLNTGLGSS
                     DDPAAVLENRRRVAVAMGGTPDDLAACYQIHSAITHVANGPWRGDRPEGDAVASATPG
                     VICAILTADCAPILLADAEAGVVAAVHAGWKGALGGVIQSAVSAMSALGAEPSRTVAV
                     VGPCIAQASYEVGSDFEDRFTQTAPGAERFFEVGDSPDKRRFDLPGFVLWRLEQAGVG
                     EAAWTGHDTCAAETLFFSNRRAFQRGEPDFGRLMSAISVNG"
     gene            complement(1414..2484)
                     /locus_tag="VF633_02940"
     CDS             complement(1414..2484)
                     /locus_tag="VF633_02940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8470046.1"
                     /translation="MSLKSRLAREIALTGPMSVADYVTRCLHDPQDGYYSTRPALGER
                     GDFITAPLISQMFGELLGLWAAETWHRLGAPERVRLVEVGPGDGTLIDDALRAMRLAP
                     DFLEACDLILIEPSAPLRKEQERRLAGADIAPRWLSSLNQIETDAPVILIANEVLDCM
                     PARQFMKTEDGWSERRVGVDDEGGILWGLSNITGGFEKPDYVVEPGQIVEISDQQAIF
                     GRDIAVMLAEASGAALLIDYGRDKPGAGDTLQALHRHTKVDPLEHPGAADLTQWADFP
                     RVLEAAVRAGADVTGCKDQSDFLRLLGIEARADRLIQGRPDAEPVIRRQLERLTAEDQ
                     MGSLFKVASIFSPRSLTVPGFD"
     gene            complement(2481..3377)
                     /gene="lgt"
                     /locus_tag="VF633_02945"
     CDS             complement(2481..3377)
                     /gene="lgt"
                     /locus_tag="VF633_02945"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX8470047.1"
                     /translation="MIFPEFDPVLIHLGPLPIRWYALAYVAGIVLGWWYAGVLIKKNS
                     LWSPRRPPIGSVQLDDLILWITLGIILGGRLGYALFYKPAMYLQLFTGDSWGERLELF
                     QLWTGGMSFHGGFLGCTLAVILYSRRQKLNTLSIGDMALSTAPIGLFFGRIANFINGE
                     LWGRETTVPWAVRFCNARIEQMYGVCPAGDVPRHPSQLYEAGLEGLLLFGILALAVWK
                     FRLLSKPGYITGLFLVGYGVIRASLENVRQPDVGFDHLPFGLTMGMILSIPMVLVGAW
                     LIWRAWKTPPVVEELEPEEAAA"
     gene            3572..3859
                     /locus_tag="VF633_02950"
     CDS             3572..3859
                     /locus_tag="VF633_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX8470048.1"
                     /translation="MQTRNPILDEFAKITTGAMGLAQAAGEEAKAAFRAQTDRVAAEM
                     DLVRRDEFDALKAEVAALRAQVTVLNAAFAATKTPETGTSTGGTPAADAAG"
     gene            3957..4460
                     /locus_tag="VF633_02955"
     CDS             3957..4460
                     /locus_tag="VF633_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8470049.1"
                     /translation="MDAVRPEEVDVPYDPLDVVEHVLNAENLPFDRTDEGDLAFALAG
                     DWKDYELWFAWRPEGDCLQLCCALDLRATKPRRNAAYELVSLVNQRTWMGHFEVWSED
                     GEIVFRHSLALPHGERPTLAQAASMIDAAVETADRYYPAFDFMIRGNKKPREAIDACL
                     FETVGTA"
     gene            4574..5389
                     /locus_tag="VF633_02960"
     CDS             4574..5389
                     /locus_tag="VF633_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEX8470050.1"
                     /translation="MNIPETNERRPVVLVGCGRLGSAIVEGWLHTGGVSPTGLIILTP
                     SSKPIAEVAVARGASLNPSPDALASARAVVLAVKPAMWRSAVADLVEILAPDAIIVSV
                     MAGVRAADISETLLGRSVARVMPTIAVATGQGVAALWSPNRVAREVAHDLFDGLADMV
                     ELDREDLIDVATAVAGSGPAYVHAFTRALAEAGEAQGLDPASALTLARGAVRSAASAD
                     RAETLDALIARVASPGGTTEGGLKALNDGGALDGAVGAAVSAALAKARELSKG"
     gene            complement(5529..6602)
                     /locus_tag="VF633_02965"
     CDS             complement(5529..6602)
                     /locus_tag="VF633_02965"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX8470051.1"
                     /translation="MNDQANNPVECQPLLCSECFQDAGLRLDAERFGLSSRQPCPNCG
                     SLTGALLDLGRIRWVAHRFFVLGSLTKVDYGGAPRIEFNDLRSNEISFEGGLQQDVDL
                     ICEKAEVGFFHYGPPLWRLGHIEPLEALQDGERAASVIQRILAEYPVFSLQPGDPFFR
                     LRKDVSRPSEKSQFDSPPNPMCGTGRLDSEDIPVLYGSSDLQLCVHECRVTVDDSLHV
                     ATLEATKPLRLLDLTAILQEDCTTFESLDMTVHLLFLAGPHSYPISRVIGAAAKEAGF
                     DGLLFPSYFSLLRTGAPFLETAYGLATRAFPDAASREAGKIVPNIALFGRPIADGRID
                     VRCINRLYLRQAAYDLGFGPADI"
     gene            complement(6659..7750)
                     /locus_tag="VF633_02970"
     CDS             complement(6659..7750)
                     /locus_tag="VF633_02970"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8470052.1"
                     /translation="MKAFLSYPSEHVEIAREVKSFIRSTGVVCWFDKDDLVAGEDWDR
                     ARSLALREADVVVVLCAAQTTERNGVYQREINEALSLRNDRRLGLVYIIPLRIEDASL
                     PPELSRLQYVDYFDPSWRRKCAASLARAVSEQNENVPPALQVAAAQPDEGGVVLREIN
                     EEIPQGTLSATWLSYALDGDYWDFVNGVIRARALGGLYQARRHLTEWWKPSGSDWELH
                     ISEHYRKGQLVSLTVGTSSYFSGAAHPNHGIQSINILGEAAGVVGAADLFDTSLDALK
                     FLTDYVNLDLRRQYGRSGEEIDLTYYVETYGWEFFDQFNFNEAGMQLNFSSMSGLPHV
                     LGYQEVYLPWQHAGHFLSPTAKRILLGSD"
     gene            8422..8787
                     /locus_tag="VF633_02975"
     CDS             8422..8787
                     /locus_tag="VF633_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470053.1"
                     /translation="MSAPRLRRPYRPKYPVNTDPYARDPWADEFLQACAKLEVMKAFK
                     EACHSRVWATARARGSRCCGQAEFDEAEAMVIAAGHPKPPADWFLWNRIFEADRPNPF
                     NGSIPLMTHEEVQAALGVV"
     gene            complement(8842..9360)
                     /locus_tag="VF633_02980"
     CDS             complement(8842..9360)
                     /locus_tag="VF633_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470054.1"
                     /translation="MRRFAFFAPLAVAAAAASLAYAEPAANQAPSPGPLVTVSVGGDL
                     TEQVEKLGERDVNQQRDELVQTVTRALVRSGDYDGAQINLVLTDLKPNRPTMQQTIDR
                     PGLSMFDSISIGGAAIEGEIITAGGQRLPVRYSRYNTSLADVFGYNTWEEANRAYDRF
                     ASNIVAGRLVSR"
     gene            complement(9466..>10399)
                     /locus_tag="VF633_02985"
     CDS             complement(9466..>10399)
                     /locus_tag="VF633_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470055.1"
                     /translation="YPAYVDILVQEPIGVLRIIAQRERAVASQAHIFVLWLMLATVLL
                     MSVAILFIRNQVRAIERLAEAAEAFGRGEVSPRFKPHGAREVRQAATAFLAMRDRIQR
                     HIEQRTALLASVSHDLRTPLTRLRLELALAPPFKRADDMKGDLDEMEHMIDEYLAFAR
                     GEAGEAAQTVDIVGLLKAAGDDARRAGAEVEVAAPTGLSASLRPIAFKRAISNLTANA
                     ASHGDHVRLTARALPSGGLEIAVDDDGPGIPDELHEEAFMPFSRLDESRNQNSKGVGL
                     GLAIARDVARGHGGDITLARSDMGGLRALIRLPG"
BASE COUNT         1905 a         3318 c         3366 g         1810 t
ORIGIN      
        1 ggcggagttg caacgttcgt ttattttgtt ggctacgctg ccaatctcgc aaaggatgcg
       61 ctctttcgga aacgcatcta aatagctgct tcccaccctt aaggatcatc cggcacgtct
      121 gcttttcggc ctgattgtca tgactgaaat cgacccgtta cggacgttaa cggcctgact
      181 ggttcaagaa ctaacgctat gccaaagtcg aaccatgcgg acttggctca tcatcacgat
      241 catgctcgga ccagtcgttg catactggct ttcgattatc agcgcggccc gtcgcctgcc
      301 gaggcaacgg cgacagatag gtgacgcccg acctgtgccg gggttgttcg atccggcgac
      361 ttcgcatcgt tggctgggga ttatcctgac cgaagatacg tcgcagtttt cgccacgtgc
      421 gaaacgagca ttctggatcg cccaactaag cgtgtgcctg atcccctttg gtttcattct
      481 ggcgatggtc attgcgaaca acggagtcga tgaacgatag aagcgcagcc cgtgagacgc
      541 ggacccccgc ccgttacgct tcccatcgag aatgaccgct ttccatccct tagtgggcat
      601 tcgagcagtc cgctttcagg cgaagaacct taccctagcc gttcacactg atcgccgaca
      661 ttaaccggcc gaagtccggc tcgccccgct ggaacgccct acggttggag aagaagaggg
      721 tttcggcggc gcaggtgtca tggccggtcc aggcggcttc accgacgccg gcctgctcca
      781 gacgccacaa gacgaacccg ggcaggtcga agcggcgctt gtcgggggaa tcgccgactt
      841 cgaagaagcg ttcggcgccg ggggcggtct gggtgaaccg gtcctcgaag tctgagccga
      901 cctcgtagct ggcttgggcg atacaggggc cgacgacggc tacggtgcgg gacggctcgg
      961 cccccagagc cgacatcgct gagaccgcgg actggatgac gccgcccagc gcgcctttcc
     1021 agcctgcatg caccgctgcc accacgcccg cctcggcgtc ggccagcagg atgggggcgc
     1081 agtcggcggt gaggatggcg cagatgacgc cgggcgtggc gctggcgacc gcgtcgccct
     1141 cgggccggtc accgcgccac gggccgttgg cgacgtgggt gatggcggaa tgaatctggt
     1201 agcaggcggc gaggtcgtcc ggcgtgccgc ccatagcgac ggcgacgcga cggcggttct
     1261 ccagcacggc ggcgggatca tcgctggagc cgagaccggt gttcaacccc tcgaagatgc
     1321 cggtcgagac cccgccctga cgtgtgaaga agccgtggcg cacgccggct ttcgtcaaca
     1381 gggggtgcac gatggggggg ggcaggtcgc tcatcagtcg aaccccggga ccgtcaggct
     1441 gcgcggcgag aagatcgagg cgaccttgaa cagggacccc atctggtctt cggcggtcag
     1501 gcgctcgagt tggcggcgga tcacgggctc ggcgtcgggt cgtccctgaa tcaggcggtc
     1561 ggcgcgggcc tcgataccca gcaggcggag gaagtcgctc tgatccttgc agccggtgac
     1621 gtcggcgccg gcgcggacgg cggcctccag aacgcgcggg aagtcggccc actgggtcag
     1681 gtcggcggcg cccggatgct cgagcggatc gaccttggtg tgccggtgca gcgcctgcag
     1741 cgtgtcgccc gcacccggct tgtcgcggcc atagtcgatc agcaaggccg cgcccgaagc
     1801 ctcggccagc atgaccgcga tgtcgcgtcc gaagatggcc tgctggtccg agatttcgac
     1861 gatctggccc ggctcgacaa cgtagtccgg cttctcgaag ccgccggtga tgttggacag
     1921 gccccacagg atgccgccct catcatcgac gccgacacgc cgttccgacc agccgtcttc
     1981 ggtcttcatg aactggcgcg cgggcatgca gtccagcacc tcgttggcga tcaggatcac
     2041 cggtgcgtcg gtctcgatct ggttcagcga gctcagccaa cgtggcgcga tgtcggcgcc
     2101 ggccagccgc cgctcctgtt ccttgcgcaa gggagcgctc ggctcgatca ggatcaggtc
     2161 gcaggcctcg aggaagtcgg gggccagccg catggcgcgg agcgcatcgt cgatcagggt
     2221 cccgtcgccc ggcccgacct cgaccagccg caccctctcc ggcgcaccca gacgatgcca
     2281 ggtctcggcc gcccacaggc ccagcagttc gccgaacatc tggctgatca gcggcgcggt
     2341 gatgaagtcg ccgcgttcgc ccagcgcggg gcgggtcgaa tagtagccgt cttgcgggtc
     2401 gtgcaggcag cgggtgacat agtcggccac cgacatcggc ccggtcaggg cgatctccct
     2461 cgcgaggcgg cttttcaggc tcacgccgcc gcctcttcgg gctccagctc ttcaacgacc
     2521 ggcggcgtct tccatgcccg ccagatcagc cacgcaccga ccagcaccat cgggatcgac
     2581 aggatcattc ccatggtcag gccgaacggc aggtggtcga acccgacgtc gggctgacgc
     2641 acgttctcca gcgaggcccg gatcacgcca tagccgacga ggaacagccc ggtgatatag
     2701 cccggcttgg acagcagccg gaatttccac accgccagcg cgaggatgcc gaacagcagc
     2761 aacccctcaa gcccggcctc atacagctgg ctggggtggc gcggcacgtc gccggcggga
     2821 cagacgccat acatctgttc gatgcgggcg ttgcagaagc ggacggccca ggggaccgtc
     2881 gtctcgcgac cccagagctc gccgttgatg aagttcgcga tccgtccgaa gaacaggccg
     2941 atcggtgcgg tggacagggc catgtcgccg atgctgagcg tgttcagctt ctgacgccgg
     3001 ctgtagagga tcaccgccag cgtgcagccg aggaagccgc cgtggaacga catcccgccg
     3061 gtccacagct ggaacagctc cagccgctcg ccccaactgt cgccagtgaa cagctgcaga
     3121 tacatcgcgg gcttgtagaa cagcgcatac cccagacgcc cgccgaggat gatgccgagg
     3181 gtgatccaca ggatcaggtc gtcgagctgg accgagccga tcggcggcct gcgtggcgac
     3241 cacaggctgt tcttcttgat caggacaccg gcgtaccacc agcccagaac gatcccggcc
     3301 acataggcaa gggcgtacca gcggatcggt aatggaccga ggtggatgag gaccggatcg
     3361 aactcgggaa agatcacgaa gggcagctcc agccgttttc agttgtgcgg tttagcaagc
     3421 gtcgcctgcg ccgtcttccc tctgcgagca tacctccacg atttcgcccg acttgacgct
     3481 ataagtcgca tggacggaaa ggtttcgttc accatatcgg gtaaccttcc ttaatacgcc
     3541 gggactgatt tgatccgggc cggagacgac catgcagacc cgcaatccca tcctcgacga
     3601 gttcgccaag atcaccactg gcgccatggg cctggcccag gcggcgggtg aggaggccaa
     3661 ggccgccttc cgcgcccaga ccgaccgcgt cgccgccgag atggacctcg tccgtcgcga
     3721 cgagttcgac gccctcaagg ccgaggtcgc cgccctgcgc gcccaggtga cggtgctgaa
     3781 cgccgcgttt gccgcgacga agacccctga gacgggaacg tccacaggcg gaactcccgc
     3841 tgcggacgca gccggttgag ccgaatctgc gaacacgttt accgtctggc gacgataggt
     3901 gagtcgcggc ccgcgccgca cgccgatcgg gtgctcttgc tgatcggaaa taactaatgg
     3961 acgccgtgag gcctgaggaa gtcgatgttc cctacgatcc gctggacgta gtcgaacacg
     4021 tactgaacgc cgaaaacctg ccgttcgacc gcaccgacga gggcgatctg gcctttgctc
     4081 tggccggcga ctggaaggat tacgagctgt ggttcgcctg gcgtcccgag ggcgactgcc
     4141 tgcagctatg ctgcgcgctc gacctgcgcg ccaccaagcc gcgccgcaac gccgcctatg
     4201 aactggtctc gctggtcaac cagcgcacct ggatgggcca tttcgaggtc tggtccgagg
     4261 acggcgaaat cgtcttccgc cattcgctgg ccctgccgca cggcgaacgc ccgacgctgg
     4321 cgcaggccgc atccatgatc gacgccgccg tcgagaccgc cgaccgctac tatccggcgt
     4381 tcgacttcat gatccgcggc aacaagaagc cacgcgaagc catcgacgcc tgcctgttcg
     4441 agaccgtcgg caccgcctga taatcctccc ccatcggggg aggtggcgtg gcgcttcttc
     4501 gcgccgtgac ggagggggcc agtacgcgca agctgtccga cgctgaccca cccgtactga
     4561 gcgcgagtcc ccaatgaaca tccccgaaac gaatgagcgc cgacccgtcg tcctcgtcgg
     4621 ctgcggccga ctgggctcgg ctatcgtcga gggctggctg cacaccggcg gggtcagccc
     4681 gaccggcctc atcatcctga cgccgtcgtc caagccgatc gccgaggtcg ccgtcgcgcg
     4741 cggagcctcg ctgaaccctt cgcctgacgc tctggcgagc gcccgcgccg tcgtcctcgc
     4801 cgtgaagccg gcgatgtggc gctccgccgt cgccgatctg gtcgagatcc tcgcccccga
     4861 cgccatcatc gtctcggtca tggcgggcgt ccgcgccgcc gatatatccg agaccctgct
     4921 cggccgctct gtcgcccggg tcatgcccac gatcgcagtg gcgacagggc agggggtcgc
     4981 cgccctgtgg tcgcccaacc gcgtggcgcg cgaggtcgcc cacgatctgt tcgacggcct
     5041 cgccgacatg gtcgagctgg accgtgaaga cctcatcgac gtcgccaccg ccgtcgccgg
     5101 ctccggcccc gcctacgtcc acgccttcac ccgcgccctc gccgaagccg gagaagccca
     5161 gggcctcgac cccgccagcg ccctgacgct ggcccgaggc gcggtgcgat ccgccgcgtc
     5221 tgcagacagg gcggagacgt tggacgcttt gatcgcccga gtggcgtcgc cgggcggaac
     5281 gaccgaaggg gggctgaagg cgttgaatga cgggggggcg ctggatggtg cggttggggc
     5341 tgcggtgagc gcggcgctgg cgaaggcgcg ggagttgtcc aaggggtgag ggcgatttcg
     5401 cgagtgtcgc ctttgcgcca gaagcagaaa ttgtcggcca ctcgggaacg tcacattcaa
     5461 acagcctacg atggtctgct tgttgacgtc ggcctatcga catattcgct gcccaagtgg
     5521 actgcgtgtc agatgtcagc gggcccgaag cctagatcat aggcggcctg ccggagatag
     5581 aggcgattga tgcaacggac gtcaatccgt ccatcggcga tcggccttcc gaacaaggct
     5641 atatttggaa cgatctttcc ggcctcgcga gacgcggcgt ccgggaaggc gcgagtggct
     5701 aggccgtagg cggtctcgag aaaaggcgct cctgtgcgta gcaggctaaa ataggacgga
     5761 aacagcaggc catcaaagcc ggcttccttt gccgcagcgc caattacgcg gctaatcgga
     5821 taggagtgcg gtcctgctaa aaataggaga tgcaccgtca tgtctaagct ttcgaacgtc
     5881 gtgcagtcct cttgaaggat ggctgtgaga tcgagaagcc ggaggggctt tgtggcctca
     5941 agtgtcgcga cgtggaggct gtcatcgacc gttactcggc attcgtgaac gcagagctgt
     6001 aggtcggagg atccgtaaag aacgggtatg tcctcactgt ccaaacgacc tgtcccacac
     6061 ataggattgg gcggactgtc aaactggctt ttttcgcttg ggcggctcac atctttgcga
     6121 agtcggaaaa agggatcgcc aggctgcagg gaaaacacgg ggtactcagc tagaatacgc
     6181 tggatgaccg aagccgcgcg ctcgccgtcc tgaagcgctt caaggggttc aatatggcca
     6241 aggcgccaga gtggcgggcc atagtgaaaa aagcccacct cagctttctc gcagatcagg
     6301 tccacatcct gttgaaggcc tccttcaaac gagatctcgt ttgatctcag atcattgaac
     6361 tcgatacgag gggcgccgcc gtagtcgacc tttgtcaggc tccccagcac aaaaaagcgg
     6421 tgagcgaccc aacggatacg tcccaagtct agcagggcac cggtcagaga cccacagttc
     6481 gggcagggct ggcgcgagga cagaccgaac ctttctgcat ccagccggag cccagcgtcc
     6541 tgaaaacatt ccgagcaaag cagcggctga cattcgactg gattatttgc ttggtcgttc
     6601 atagacattc gctggcccta ctttcccaaa tctgatgccc cacaactgcg tgtttcgtct
     6661 aatcggatcc cagcagaatt cgctttgcag tcggtgagag gaaatgtccg gcgtgctgcc
     6721 aaggcagata aacttcctga tagccgagga catgaggcag tcccgacatg gaagaaaagt
     6781 tcagttgcat acccgcctcg ttgaagttga actggtcgaa gaattcccag ccgtacgtct
     6841 cgacgtagta agtgagatcg atctcttcgc cagacctgcc gtactgacgt ctcagatcca
     6901 gattaacgta gtcggtgaga aacttcaggg cgtccaacga tgtatcgaac aggtcggctg
     6961 caccgacaac tcccgcagct tcgccgagga tatttatcga ctgaatgcca tggttggggt
     7021 gggctgcgcc tgagaagtaa ctcgacgtcc caactgtcag ggatacgagc tgtcccttgc
     7081 gatagtgctc tgaaatgtgc agctcccagt cggacccgga aggcttccac cactcagtga
     7141 gatgacgtct tgcttggtac aggccgccta gggcgcgggc gcgaatgacc ccgttcacaa
     7201 agtcccagta gtcgccatcc aacgcgtagc tcagccatgt cgcggagagc gtcccctgcg
     7261 gtatctcttc gttgatctcg cgcagcacta cgcctccctc gtccggttgg gcggctgcga
     7321 cctgaagtgc gggcggaaca ttctcgttct gctcgctgac agcgcgagct aggctcgccg
     7381 cgcactttcg acgccaagat gggtcgaagt agtcgacgta ctgcagtcgg ctcagttctg
     7441 ggggcagcga cgcgtcctcg atccgcaacg gaatgatata cacaaggccg agccgtctat
     7501 cgtttctgag gctaagggcc tcattgattt cacgttggta gacgccgttc ctttccgtgg
     7561 tctgcgcagc gcacaggacc acgaccacat cagcctcacg aagagctagg cttctggccc
     7621 gatcccagtc ctcgcccgcg acaaggtcgt ctttatcgaa ccagcaaacg actcctgtcg
     7681 agcgaatgaa gctcttaact tcgcgtgcga tctcgacgtg ttctgatggg tagctgagga
     7741 atgctttcat aggtggccct gactctctgg ggctcatgct aatgtcctca ggagtcatga
     7801 gcgagtgggc gcgatagaat tcggtttcag aaaactggcg aagtccaagc agcccgtgca
     7861 gggctagcgc ttcaaatttg aagaggacga tcaggcaccc gccctacggt cattctgaac
     7921 gtccgcaatg aatcaggatt ggaccgtaac cgccagaaac cacgagcccc cgcaattccc
     7981 gctcccaatc ccaatgattt cccgggtttc cggcaatctg accctgctga actccgccca
     8041 cccgtcagcc gccctataat gctgccgttc cgtcgcatcg gggaggcgca gggccttgcg
     8101 agcttcgcgc ggcgggatgt gaagaagggg gaagtccagc cctgaggttg gacgacaggg
     8161 gcgaggggga tactcagccc ggtccgggga ctgcgttcgc aagacgcagc ccgcccgtgg
     8221 gaggcgctgc gctaaggcga taaaaccgcc acctggtgcg agcttccgga aaattttcac
     8281 gcggggccaa aggcggcgtc gaaacggtat tcttccattt ccttccggac gtcgtccgga
     8341 ggtcccgcgc gcctccccaa ctcaccgatc actcgggcga gcgcacagct gcgtccgggg
     8401 ccaacccaag gagacctgcc gatgtccgcc ccacgcctgc gccgtcccta tcgaccgaaa
     8461 tacccggtga acacggatcc ctacgccagg gacccgtggg ccgatgagtt cctgcaggcc
     8521 tgcgccaagc tggaggtaat gaaggcgttc aaggaggcct gtcactcgag agtctgggcc
     8581 acggcccgcg cccggggctc acgctgctgc ggacaggccg agttcgacga ggccgaggcc
     8641 atggtcatcg ctgcgggcca ccccaagccg cctgccgact ggttcctgtg gaaccggatc
     8701 ttcgaggccg accgcccaaa cccgttcaac ggcagcatac ccctgatgac ccacgaggag
     8761 gttcaggcgg cgctgggtgt tgtctgagaa aatcatcgtc atggccgaga cgagcccggc
     8821 catgacggaa ggggtatagc gttagcgcga gaccagacgc cccgcgacga tgttcgaggc
     8881 gaagcggtcg taggcgcggt tggcttcttc ccaggtgttg tagccgaaaa cgtcggcgag
     8941 gctggtgttg taacgggaat agcgaaccgg caggcgttgg ccgccggcgg tgatgatctc
     9001 gccctcgatg gcggcgccgc cgatggagat gctgtcgaac atcgacaggc cgggtcggtc
     9061 gatcgtctgc tgcatggtcg ggcggttggg cttgaggtcg gtcaggacca gattgatctg
     9121 ggcgccgtca tagtcgccgc tgcggacgag ggcgcgggtg accgtctgga ccagttcgtc
     9181 acgctgctgg ttgacgtcgc gctcacccag cttttcgacc tgctcggtca ggtcgccgcc
     9241 gacgctgacg gtgaccaggg ggccggggct cggggcctga ttggcagcgg gctcggcgta
     9301 ggccagcgag gcggcggccg cagcgacggc gaggggggcg aaaaaggcga agcggcgcat
     9361 gatgatctcc gtgatcctgt ctggagtgaa gatgagcctt ggaaccgctc ttcgccagat
     9421 gccgcgccga aaagtgatcg tccgtgacac tatgtcttcg gtgggtcagc ccggcaggcg
     9481 gatcagcgcc ctcagtccgc ccatgtcgct acgggcgaga gtgatgtcgc cgccatggcc
     9541 gcgcgccaca tcgcgggcga tggcgaggcc gagaccgacg cccttggagt tctgattgcg
     9601 gctctcatcc agccgcgaga aaggcatgaa cgcctcctcg tgcagttcgt cggggatgcc
     9661 ggggccgtcg tcatcgaccg cgatctcgag cccgcccgac ggtagcgccc gagccgtcag
     9721 ccggacatga tccccatgcg aggccgcgtt ggccgtcaga ttgctgatcg cccgcttgaa
     9781 ggcgatgggg cgcagcgagg cgctcagtcc ggtcggcgcg gcgacctcga cctcggcgcc
     9841 ggctcggcgg gcgtcgtcgc cggcggcctt gagcaggccg acgatatcga ccgtctgggc
     9901 cgcttcaccg gcttcgccac gtgcgaaagc cagatactcg tcgatcatgt gctccatctc
     9961 gtcgaggtcg cccttcatgt cgtcggcgcg cttgaacggc ggggcgaggg cgagctccag
    10021 acgcaggcgg gtcaacggcg tacgcaggtc gtggctgacc gaggccagca gggctgtgcg
    10081 ttgttcgatg tggcgctgga tccggtcgcg catggcgagg aacgccgtcg cggcctggcg
    10141 gacttcacga gcgccgtggg gcttgaaccg gggcgagacc tcgccgcgcc cgaacgcctc
    10201 cgccgcctcg gccagccgtt cgatggcgcg gacctgattg cggatgaaca ggatggccac
    10261 cgacatcagc aacaccgtcg ccagcatcag ccacagcacg aagatatggg cctgcgacgc
    10321 cacggcccgc tcacgctgcg cgatgatccg caacaccccg atgggctcct gcaccagaat
    10381 gtcgacatag gcgggatag
//
LOCUS       DASVTM010000104         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_647620,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000104 DASVTM010000000
VERSION     DASVTM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647620"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VF633_02990"
     CDS             complement(<1..378)
                     /locus_tag="VF633_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8470056.1"
                     /translation="MLAALTLASCDRGQTDAGTAEPAATSAIPKAGTVEAQSPTLAAV
                     KRRGYLNCGVHQGLVGFAYIDNRGDWRGFDADFCRVMAASIFGDPDRVRFVPLTADNR
                     FDALAAGRIDVLWRNTSWTMDRDI"
     gene            515..1690
                     /gene="metC"
                     /locus_tag="VF633_02995"
     CDS             515..1690
                     /gene="metC"
                     /locus_tag="VF633_02995"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX8470057.1"
                     /translation="MKTPSDRTRLIAGATRRGGGRRPVNPPIERASTMLSDRVGDMRD
                     ESLGPTYGLTSGSAARELRAAVAGLEGADDAWIVPSGLAAVTVPLLAVARPGDEILAS
                     DAVYGPTRRFLSRWMGGRGVSVRYHPAEADAATVASLIGERTRVVLIESPASLTMEML
                     DVPALAAAAQAKGVLTIMDNTWAAGLAFKPLAHGVNISVQALTKYASGHSDVLIGSIA
                     TNDAVLGRAVHDTIEDMGWHVSPDDAWLTLRGIRTLPLRMTEQARSALQVTAWLRDQP
                     EVAEVLYPPLPGSAGHDLWARDYTGAASLFGVVMTGGTTASAEAMLDSLSLFGLGYSW
                     GGFESLATHEKHQMDYRQQPPTLAGELLRFHIGLEDPADLIADLEVGLAAWRMSLRT"
     gene            complement(1687..1950)
                     /locus_tag="VF633_03000"
     CDS             complement(1687..1950)
                     /locus_tag="VF633_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470058.1"
                     /translation="MAETKNQTLRGVIKLDGATFRDCEFVDAQLTYEGGTPPNFINCR
                     FTNSNFAFVESAGNTVNFLRAMAPHASNMREVVFGLMPELKDN"
     gene            complement(1991..2067)
                     /locus_tag="VF633_03005"
     tRNA            complement(1991..2067)
                     /locus_tag="VF633_03005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2031..2033),aa:Arg,seq:tct)
     gene            complement(2126..2467)
                     /locus_tag="VF633_03010"
     CDS             complement(2126..2467)
                     /locus_tag="VF633_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470059.1"
                     /translation="MLEHLSDPVAALASISRHAADRIVLGVYIPEATETAQAWFLSSA
                     DRPESSAVHWAYSMPVYREVLAMLGFEIESVTARCFQHVAGPSPRTTIVARRSAAQRS
                     CGRPMAGPTVD"
     gene            complement(2398..>2814)
                     /locus_tag="VF633_03015"
     CDS             complement(2398..>2814)
                     /locus_tag="VF633_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470060.1"
                     /translation="DYGAYTGNVDMIGKSVLDIGTASGFLSFEAEAAGASEVVSFDMD
                     TAERQILQPFNDSLYFTNHAAWVGEQTAVIRRWKMGYWTAHHALASKARAVYGNIYRL
                     PRPSASLMSSSSAPCLSTFQTRLRLWPQSRVTPLTA"
BASE COUNT          492 a          867 c          960 g          495 t
ORIGIN      
        1 gatgtcgcga tccatcgtcc atgacgtatt gcgccacagg acgtcgatcc gaccggccgc
       61 cagcgcgtcg aacctgttgt ccgccgtgag gggcacgaac cggaccctgt ccggatctcc
      121 gaagatcgaa gcggccatga cccggcagaa gtcggcgtcg aacccgcgcc agtcgccgcg
      181 attgtcgatg taggcgaacc cgaccagccc ctgatgcacg ccgcagttca gatagccgcg
      241 ccgtttgacg gcggccagcg tggggctttg cgcctcaacg gttcccgcct tgggaatggc
      301 gctggtggcg gcgggctcgg cggtcccggc atcggtctga ccacgatcgc aggaggccaa
      361 tgtcagggcg gcaagcatca ggagcttcag acccagccgg gttccgcttc gtcctgtact
      421 cgctgtcgtc cgcatgcccg gcctcgcaac tgtgctgttc cgttagaagc ctttaggcct
      481 tgatccgcaa cccgccgccc gtcatcgagg aggcatgaaa acgccttcgg accgcactcg
      541 actgatcgct ggagccaccc gccgcggcgg gggacggcgg cccgtcaatc cgcctatcga
      601 gcgcgcctcg accatgctca gcgaccgggt cggcgacatg cgcgacgaaa gcctcggtcc
      661 gacctatggc ctgaccagcg ggagcgctgc gcgtgagctt cgcgctgccg tggccgggct
      721 ggaaggcgcc gacgatgcgt ggatcgtgcc gtcggggctg gccgccgtga ccgtgccgtt
      781 gctggcggtg gccagacccg gagacgagat tctggcctcg gacgccgtct atggtcccac
      841 tcgccggttc ctgtcgcgct ggatgggcgg acgcggggtc tccgtaagat atcaccctgc
      901 cgaggccgac gcggcgacgg tggcctcgct gatcggcgag cggacccgcg tggtgctgat
      961 cgaatcgccc gcttcgctga cgatggagat gctcgacgtg cccgcgttgg cggctgcagc
     1021 gcaagccaag ggcgtgctga ccatcatgga caatacctgg gccgccgggc tggctttcaa
     1081 gccgctggcc catggcgtca acatcagcgt gcaggccctg accaagtacg cgtcgggcca
     1141 ttcggatgtg ctgatcgggt cgatcgcgac caatgacgct gtcctcgggc gcgcggtcca
     1201 cgacacgatc gaggacatgg gctggcacgt gtcgccggat gatgcctggc tgacgttgcg
     1261 cggtattcgg accctgcctt tgcgtatgac cgagcaggca cggtcggcgc tgcaggtcac
     1321 cgcgtggctg cgcgaccagc cggaagtggc ggaggtgctc tatcctcccc tgcccggttc
     1381 ggcgggccac gatctgtggg cgcgcgatta tacaggggcg gcgtcgctgt tcggggtagt
     1441 catgacgggc ggaacgaccg cttccgccga ggcgatgctc gatagcctga gcctgttcgg
     1501 gctggggtat tcatggggcg ggttcgaaag cctcgccacc cacgagaagc atcagatgga
     1561 ttaccgtcag cagcccccga ccctggccgg agaactgctg cggtttcaca tcgggcttga
     1621 ggacccggcc gacctgatcg cggatctgga ggtcggtctc gccgcttggc ggatgtctct
     1681 taggacctag ttgtccttga gttccggcat caggccgaag acgacttcgc gcatgttcga
     1741 cgcgtgggga gccatggcgc gcaggaagtt gacggtgttg cctgcggatt cgacgaaggc
     1801 gaaattcgag ttcgtgaagc ggcagtttat gaagttcggt ggcgtgccgc cctcgtaggt
     1861 cagttgcgca tcaacaaact cgcagtcgcg gaaggtcgcg ccgtccagct tgataacgcc
     1921 cctgagcgtc tggttcttcg tctcggccat caaagcttcc tgagctcgcg gtaaaccgcg
     1981 cgatagagaa tggcgcgccc agcaggactc gaacctgcaa catcccgctt agaaggcggg
     2041 tgctctatcc ggttgagcta tgggcgcgga cagggtggaa aatcaggccg gataaggggt
     2101 tgcgggtcaa ggcgcaaggg ctgggctaat cgacagtcgg acccgccatc ggacgaccgc
     2161 aagacctctg ggctgcggac cgccgcgcta cgatggtcgt acgcggcgag gggccagcga
     2221 cgtgctggaa gcaccgtgct gtcacgctct cgatttcgaa gccgagcatg gccagcactt
     2281 cacgataaac cggcatcgaa taggcccaat gcacggcgga cgattccggc cgatctgcgg
     2341 agcttaagaa ccacgcctga gcggtctcgg tggcctcagg gatgtagacg cccagaacta
     2401 tgcggtcagc ggcgtgacgc gagattgagg ccagagccgc aaccgggtct gaaaggtgct
     2461 caagcacggc gcagacgagg acgacatcaa actggccgag ggccggggga gccggtagat
     2521 gttaccgtag acggcgcggg ccttcgatgc gagggcgtga tgcgcggtcc aatagcccat
     2581 cttccagcgc cggataacgg cggtctgctc tccaacccat gcggcgtggt tggtaaaata
     2641 gaggctgtca ttgaagggtt ggagaatctg gcgctcggcg gtgtccatgt cgaacgaaac
     2701 gacttctgag gcgccggcag cctcggcctc gaagctcaga aatccagagg cggtcccgat
     2761 gtcgaggact gacttcccaa tcatatcgac gttgccagta taggctccat agtc
//
LOCUS       DASVTM010000105         9839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_64055, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000105 DASVTM010000000
VERSION     DASVTM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64055"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /gene="hemE"
                     /locus_tag="VF633_03020"
     CDS             <1..1006
                     /gene="hemE"
                     /locus_tag="VF633_03020"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX8470061.1"
                     /translation="SGQKTDRPPVWFMRQAGRYLPEYRALRAEAPDFIAFCLNPEMAA
                     EATLQPMKRFGFDAAIVFADILLIPQALGQDVWFETGEGPKLGEMPVVGRMAELAEGA
                     GDYLHQVGQTLSLVREGLEPERALIGFAGAPWTVATYMLDGHHNTIGKGERATARKYA
                     YEDPERVDALLEVLVEATARYFVMQANAGAQVLKIFESWAEGLPDDLFERLVLNPHKA
                     LVARTRELGVDVPLIGFPRGSAIHAERYATEVDVQAVALDTACPLEAGKRVQKIKPIQ
                     GALDPLLLRVGGKALDARVDQLMEAWGDGPWIFNLGHGILPDVPIAHVEQVLKRIGAQ
                     "
     gene            1006..2082
                     /gene="hemH"
                     /locus_tag="VF633_03025"
     CDS             1006..2082
                     /gene="hemH"
                     /locus_tag="VF633_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX8470062.1"
                     /translation="MATGEVSHSGQDAGGRRIAVVLFNLGGPDDAASVKPFLFNLFSD
                     PAIIGLPDFLRLGVAKLISSRRETSAQANYAMMDAGGGSPLLPETRRQSSALETALTS
                     ALPEDEIQVFIAMRYWHPLTEETAVDVAAFGPDEVVLLPLYPQFSTTTTQSSLKAWNE
                     SYAGSGRSRAICCYPQGDGWIKAQAESIRSVLTETGDRPIRVLFSAHGIPEKLVTKGD
                     PYQEQIESTVAAIVAQLGPVDYRICYQSRVGPMKWLGPSTPEAIAEAGRDQVGAVVVP
                     VAFVSEHIETLVELDIEYGELAHKLDVSPYLRTPTVSLSEPFITMLSNAVIGALGRTG
                     AAPYGPGCQASWKACPFQHQEKAA"
     gene            2079..2546
                     /locus_tag="VF633_03030"
     CDS             2079..2546
                     /locus_tag="VF633_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX8470063.1"
                     /translation="MNAYDIARGLHILAVIAWMAGLLFLPRLYAYDAEQAAKPEPLKS
                     EMQTLLRLWQARLMKIILNPAAGLALIFGAWLIHLDVQMRGASFLAEPWMIAKLVGVV
                     LLFGWHGFLSAERKRIAVGTSTKSSKFWRMTNEIPFVLAIIMVLSVTTEWTFG"
     gene            2965..4404
                     /gene="rho"
                     /locus_tag="VF633_03035"
     CDS             2965..4404
                     /gene="rho"
                     /locus_tag="VF633_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX8470064.1"
                     /translation="MTDVRDTDPTALEAEALEGQNADGASEVETTAEEAAASDAQIEA
                     AEIEDEEEGDAVVPSGRITLQELNDKTPADLVAFAEQLEIENAGNLRKQDLLFAILKA
                     LADEEVEIIASGVLEILSDGFGFLRSPDANYLPGPDDIYVSPSQIRRFGLRNGDTVHG
                     AVRGPREGERYFALLKVDTINFEDPEAVSTKVLFDNLTPLYPEERLHMEIQDPTIKDR
                     SGRVIDIVAPLGKGQRCLIVAPPRVGKTVMLQNIAKSIERNHPEVFLIVLLIDERPEE
                     VTDMQRTVKGEVVASTFDEPASRHVAVAEMVIEKAKRLVEHKRDVVILLDSVTRLGRA
                     YNATVPSSGKVLTGGVDANALQRPKRFFGAARNVEQGGSLTIIATALIDTGSRMDEVI
                     FEEFKGTGNSEIVLDRKVADKRIFPAIDVLKSGTRKEELITPKDQLTKTYVLRRILNP
                     MGPQDAIEFLLDKLRQSKNNSDFFQSMNT"
     gene            4481..4714
                     /locus_tag="VF633_03040"
     CDS             4481..4714
                     /locus_tag="VF633_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HEX8470065.1"
                     /translation="MKVTVNVECEPAEARAFLGLPNVEPLNDHLVAEMKKRMDANMSA
                     MQPDELMKSWTSFGLQAQDQFRKLMEAAATTTK"
     gene            4794..6101
                     /gene="mnmE"
                     /locus_tag="VF633_03045"
     CDS             4794..6101
                     /gene="mnmE"
                     /locus_tag="VF633_03045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX8470066.1"
                     /translation="MTQQTIFALATPPGRGAIAIIRLSGPATDAALTALGAAKLKPRT
                     ASLRVLRHGKTVIDQALILRFPAPNSYTGEDSAELHLHGGRAVVEAASNALIALGLRP
                     AEPGEFTRRAFQNGRMDLAQAEAIADLIDAETTAQASQALGQLDGALSQTYAGFRRDL
                     LKALALVEAEIDFPDEDVPDNLARTAGPVLDALAGDLRAGLADSDRGRRVREGYRIVL
                     IGETNAGKSSLFNALVEREAAIVTPIAGTTRDILDAELVIGGYAVTLSDTAGLRDSED
                     VIEREGVRRARVRAEEADLRLWIRAPGDPEGVAAEFAKSDDLVVYTKADLDQTSGAVA
                     GSIAVSTLTGEGLSSLHDWISARLAHDLSGSDFPAVTRERHRRRLSEALAAVDAGRLA
                     LNIAPEMAGDDLRRAADALSRVTGAIGVEDVLGEVFSTFCMGK"
     gene            6171..8048
                     /gene="mnmG"
                     /locus_tag="VF633_03050"
     CDS             6171..8048
                     /gene="mnmG"
                     /locus_tag="VF633_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX8470067.1"
                     /translation="MNSPTSFDVIVIGGGHAGCEAAAASARAGARTLLLTQKLETVGE
                     MSCNPAIGGLGKGHLVREIDALDGVMGRLGDVSGIQFRLLNRSKGAAVQGPRSQIDRR
                     LYREAMQAELAQTPNLTMIAGTAEGLIMDGDRVVGVTTNSGETIRAAAVVLTTGTFLN
                     GVIHRGDERIPAGRHGEEPSTGLAADLYGAGLHMGRLKTGTPARLDGRTIGWDRLEMQ
                     AADDEPVPFSFLTERIEVPQIACGITSTNEETHKIIAANLGESAVYGGHLTGRGPRYC
                     PSIEDKVVRFADKTSHQVFLEPEGLDDPTVYPNGISTSVSPETQLAFLRTMPGLEAVE
                     VFRFGYAIEYDYVDPRELTPALEVKRRPGLYLAGQINGTTGYEEAGAQGLIAGLNAAR
                     AAAGSDPIILGRDQAYIGVMIDDLVTRGVTEPYRMFTSRAEYRLTLRADNADQRLTPV
                     GIAAGIVSSDRTARFGAKAAMLAEAATLVRETRYTPKEAQALGIAVNADGQRRSIREL
                     LGYPGVDLTAFETVHPIVSTWSTVVREQVQIDAGYAGYLDRQSADADALRREEQLKLP
                     ANLDYATIGGLSNEVREKLMLIRPQTLGQAGRIEGMTPGALTALLSYVRKGVSRETVA
                     A"
     gene            8048..8671
                     /gene="rsmG"
                     /locus_tag="VF633_03055"
     CDS             8048..8671
                     /gene="rsmG"
                     /locus_tag="VF633_03055"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX8470068.1"
                     /translation="MTPEEFQDLTGASAERMADLVAFHGHLTEANAVMNLVGPGSMPD
                     FWNRHAWDSAQLLELAPDAKTWADLGAGAGFPGVVLAILLKDDPDAHVWLVDSLQKRC
                     KFLQYVVDELELPATVIWGRAEEQELEVDIVTARAVASLDKLVGYAQPYLSRGAQALF
                     LKGEKAEQELIDARKVWHLDAELSISRSDPRGRIVTVRTLRRGKRFA"
     gene            8652..9476
                     /locus_tag="VF633_03060"
     CDS             8652..9476
                     /locus_tag="VF633_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8470069.1"
                     /translation="MANVSPEKKTRVLAVSNQKGGVGKTTTAINLGTALAAIGEKVLI
                     VDMDPQGNASTGLGVPRETRRITVYDVIVDGRSVHDAAVHTAVPGLDIIPADADMSGV
                     EIELSQADRRSYRLRDALAAQGDAGHTGYDYVLIDCPPSLNLLTLNAMAAADGVLVPL
                     QCEFFALEGLTQLMKTIEMVRQSLNPSLEIQGLVLTMYDRRSALSGQVAADVRSHFGE
                     KVYDAVIPRNVRVAEAPSFGKPVLIYDLKCTGSQAYLRLAKEVVGRERRRRAQLAA"
     gene            9504..>9839
                     /locus_tag="VF633_03065"
     CDS             9504..>9839
                     /locus_tag="VF633_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX8470070.1"
                     /translation="MSERQRGLGRGLAALMGEDANEAAPATGGPATGVQTAPIESLKP
                     NPDQPRKIFTQENLDELTNSIRDKGILQPILVRTQPGQDGMWQIIAGERRWRAAQAAR
                     LAVVPIVVRE"
BASE COUNT         1804 a         3178 c         3084 g         1773 t
ORIGIN      
        1 ttcaggccag aagacagatc gtccgcccgt ctggttcatg cgtcaggccg ggcgctacct
       61 gccggaatac cgggctcttc gggccgaggc tcccgacttc atcgccttct gcctcaatcc
      121 cgagatggca gccgaggcga cccttcagcc gatgaagcgg ttcggcttcg acgccgcgat
      181 tgtcttcgcc gatatactgc tgatccctca ggcgctgggc caggacgtct ggttcgaaac
      241 cggggagggc cccaagctcg gcgagatgcc ggtcgtcggt cgtatggcgg aactggcgga
      301 aggcgcaggc gactacctgc atcaggtcgg ccagaccctg tcactggttc gtgaaggtct
      361 cgagccagaa cgcgccctga tcggtttcgc cggcgcaccc tggacggtcg cgacctatat
      421 gctggacggc caccacaaca cgatcggcaa gggcgaacga gcgaccgccc gcaaatacgc
      481 ctatgaggat cccgagcgcg tcgacgccct gctcgaggtt ctggtcgagg ccaccgcccg
      541 ctatttcgtc atgcaggcca acgccggcgc ccaggttctc aagatcttcg agagctgggc
      601 cgagggtttg cccgacgacc tgttcgaacg tctggtgttg aatccccaca aggctctggt
      661 cgcccgcacg cgtgagcttg gcgtcgatgt tccgctgatc ggcttcccgc gcgggtcggc
      721 catccacgcc gaacgctatg ccaccgaggt cgatgtccag gccgtcgcgc tcgacaccgc
      781 ctgtccgctc gaggccggaa agcgcgtgca gaagatcaag ccgatccagg gcgcgctcga
      841 tcccctgctg ctgcgggtcg gcggcaaggc gctggacgct cgcgtcgatc aactgatgga
      901 ggcctggggc gacgggccgt ggatcttcaa ccttggccac ggcatcctgc ctgacgtgcc
      961 gatcgcccac gtcgaacagg tgctgaagcg gatcggagcc caataatggc gaccggagag
     1021 gtatcccatt cagggcagga tgcaggcggc cgccggatcg cggtagtgct gttcaatctg
     1081 gggggcccgg acgacgccgc ctcggtgaag ccgttcctgt tcaacctgtt cagcgacccc
     1141 gcgatcatag gtctgccgga cttcctgcgc ctgggcgtgg cgaaactgat ctcatcgcgc
     1201 cgcgagacct cggcccaggc caactatgcg atgatggacg ccggcggcgg ttcgcctttg
     1261 ttgcccgaga cacgccgaca gtcctcggcg ctcgagacgg ccctgacgtc cgctcttccg
     1321 gaagacgaga tccaagtctt catcgccatg cgctactggc atcctctgac cgaggagacc
     1381 gccgtcgacg tcgccgcctt cgggcctgac gaggtcgtgc tgctgccgct ctatccgcaa
     1441 ttctcgacca ccacgaccca gtcttcgctc aaggcctgga acgaaagcta cgccggctca
     1501 ggccgctcgc gggccatatg ctgctatccg caaggcgacg gttggatcaa ggcccaggcc
     1561 gaaagcatcc gttccgttct caccgagacc ggcgatcggc ctatacgggt cctgttctca
     1621 gcccacggca tccctgaaaa gctggtcacg aagggcgatc cctatcagga acagatcgag
     1681 tcgacggtcg ccgccatcgt cgctcaactg ggtccagtcg attaccgcat ctgctaccag
     1741 agccgcgtag ggccgatgaa gtggctgggc ccctccacac ctgaggccat agccgaagcg
     1801 ggcagagacc aggtcggggc agtggtcgtt ccggtcgcct tcgtctccga gcatatcgag
     1861 accttggtcg agctggacat cgaatacggc gaactcgccc acaagctcga cgtcagcccg
     1921 tatctgcgca ccccgacggt cagcctttcc gagcccttca tcaccatgtt gtcgaacgcg
     1981 gtgatcggcg ccttgggccg aacgggagcc gctccctacg gtcccggctg tcaagcctcg
     2041 tggaaagctt gcccatttca acaccaggag aaggccgcat gaacgcctat gatattgcac
     2101 gcggtctcca catcctcgca gtgatcgcct ggatggccgg actgctgttt ctcccgcgcc
     2161 tctatgccta tgacgccgaa caggcggcca agcccgagcc gctgaagtcc gaaatgcaga
     2221 ccctgctacg actgtggcag gcgcggctga tgaagatcat tctcaacccc gcggcgggcc
     2281 tggcgctgat attcggcgcc tggctgatcc atcttgacgt ccagatgcgc ggcgcatcct
     2341 tcctcgccga gccgtggatg atcgccaaac ttgtcggcgt cgtgctgctg ttcggctggc
     2401 acggcttcct ctcggccgag cgcaaacgga tcgccgtcgg gacctcgacg aaatccagca
     2461 agttctggcg catgaccaac gagatcccgt tcgtgctggc gatcatcatg gtgctgtcgg
     2521 tcacgacgga atggacgttt ggctaaacct tccctctccc ggcgggagag ggcttgagcc
     2581 tccgggagcg tagcgatcgg caaggcgaaa gggtgagggg ttaaggttga acgggagtcc
     2641 ccctccgtcg gacacgataa cccctcaccc tttcgcgcca gaacgacggc ttcgccgcca
     2701 tgcgcgcaag ccctctcccg ccgggagagg gttctcgcga catccagcac cgcttgacct
     2761 gacaccgcgt ttcgtggttt cgctggcggt gaggcgtggc tccggctgcg ctgcccttct
     2821 ctatgcgacg accggtgcgc ctggcttctt ttagccgccg ccggacccga cgccttccct
     2881 ccggcccaca aggccacatt ttccaacgcc tttccgtccc gccccttctt ggcgtgtgga
     2941 tgcgaggctg agtgctatcc tgacatgacc gacgttcgcg ataccgatcc caccgctctc
     3001 gaggccgaag cgctcgaagg ccagaacgct gatggggcct ctgaggtcga gaccacggcc
     3061 gaagaagccg cagcgtcaga cgcccagatc gaggccgccg aaatcgaaga tgaggaggag
     3121 ggcgacgccg tcgtcccgtc cggccggatc acgctgcagg aactgaacga caagacgccg
     3181 gctgatctgg tcgctttcgc cgaacagctc gagatcgaga acgccggcaa cctgcgcaaa
     3241 caggacctgc tgttcgccat cctcaaggcc ctggccgacg aagaagtcga gatcatcgcc
     3301 tcgggcgtgc tcgaaatcct ctccgacggc ttcggcttcc tgcgcagccc ggacgcgaat
     3361 tatcttccgg gcccagacga tatctacgtc tcaccctcgc agatccgccg cttcggcctg
     3421 cgcaacggcg acaccgtcca cggggccgtt cgtgggccgc gtgaaggcga gcgctacttc
     3481 gccctgctta aagtcgacac gatcaatttc gaggatccgg aagcggtctc gaccaaggtc
     3541 ctgttcgaca acctgacgcc gctctatccc gaagagcggc tgcacatgga aatccaggat
     3601 ccgacgatca aggatcgctc gggccgggtc atcgacatcg tcgccccgct cggcaagggt
     3661 cagcgttgcc tgatcgtggc tccgccgcgc gtcggcaaga cggtgatgct gcagaacatc
     3721 gccaagtcga tcgagaggaa ccaccccgag gtcttcctta tcgtgctgct gatcgacgaa
     3781 cgccccgaag aagtcaccga catgcagcgc actgtgaagg gcgaggtcgt tgcctcgacc
     3841 ttcgacgagc cggccagccg ccacgtcgcc gtcgccgaaa tggtgatcga aaaggccaaa
     3901 cgcctggtcg agcacaagcg cgacgtcgtc atcctgctgg actctgtcac ccgtctcggc
     3961 cgcgcctata acgctaccgt cccttcgtcg ggcaaggtcc tgaccggcgg cgtcgacgcc
     4021 aacgccctgc agcggcccaa gcgcttcttc ggcgcggcgc gtaacgtgga gcagggcggt
     4081 tcccttacca tcatcgccac cgccctgatc gacaccggat cgcggatgga cgaggtgatc
     4141 ttcgaagagt tcaagggcac cggtaactcg gaaatcgttc tggaccgcaa ggtcgccgac
     4201 aagcgcatct tcccggccat cgacgtactc aagtccggca cccgcaagga agagctgatt
     4261 acgccgaagg accagctgac caagacctac gtgctgcgcc gcatcctgaa cccgatgggc
     4321 cctcaagacg cgatcgagtt cctgctcgac aagctacggc agtcgaagaa caacagcgac
     4381 ttcttccagt cgatgaacac ctgaggtcgg gagcgcggta tgactttagt cataccgcgt
     4441 ttcacgtgaa acatgggaag tgcgacgagg agcttcgatg atgaaagtca cggtcaacgt
     4501 agaatgcgaa cccgccgagg cccgtgcatt cctgggactc ccgaacgtcg aacccctcaa
     4561 cgatcacctc gtcgccgaga tgaagaagcg catggacgcc aacatgtccg cgatgcagcc
     4621 agacgaattg atgaagtctt ggaccagctt cggtcttcag gcgcaggacc agttccgcaa
     4681 actcatggaa gcggcggcga ctactaccaa gtgacccaga catctcctct ccatgcgcag
     4741 catgggaagg tggcacggcg ccgcttgcgc cgtgacggag gggcctcttc cctgtgaccc
     4801 aacaaaccat cttcgccctc gccaccccgc ccggccgagg cgccatcgcc atcatccgtc
     4861 tgtccggtcc ggccaccgac gcggcgctca ccgcgctcgg cgcagcgaag ctcaagcccc
     4921 ggacggcgtc gctccgagtg ctaaggcacg gcaagacggt catcgatcaa gccctgatcc
     4981 tcaggttccc ggctccaaac tcctacaccg gcgaggacag cgccgagctt caccttcacg
     5041 gcggtcgcgc cgtggtcgag gccgcatcca acgccctgat tgcgctgggc ctgcggcctg
     5101 ccgaaccggg tgaattcacg cgtcgcgcgt tccagaacgg ccgcatggac ctcgcccaag
     5161 ccgaggccat cgccgatctc atcgacgcgg aaacaacggc tcaagccagc caggcattag
     5221 gccagctcga cggcgcgctc agccagacct atgctggctt ccgacgcgac ctgctcaagg
     5281 ccttggcgtt ggtcgaggct gagatcgact ttcccgacga ggacgttcca gacaatctcg
     5341 cccggaccgc cggacccgtg ctggatgcct tggctggcga tctgcgcgct ggtctcgccg
     5401 actccgaccg gggccgccgc gtgcgcgagg gctatcgcat cgtcctgatc ggtgagacca
     5461 acgccggcaa gtcatcgctg ttcaacgcct tggtcgaacg ggaagccgcg atcgtcacgc
     5521 cgatcgcggg caccaccaga gacattctgg acgccgaact cgtcatcggc ggatatgcag
     5581 tgacgctctc agacactgcc ggcttgcgtg acagtgagga tgtgatcgag cgcgaaggcg
     5641 ttcgtcgggc cagggttcgc gctgaagagg ctgatctccg gctctggatt cgagctcccg
     5701 gagatcccga aggtgtagcg gctgagttcg caaaatcgga tgatctggtc gtctacacaa
     5761 aggcggatct ggatcaaact tcaggtgcag tcgccgggtc gatagcggtc agcacattga
     5821 cgggggaggg gctgtccagt ctgcacgact ggatctctgc gcgtctggct cacgacctgt
     5881 cgggctccga cttccccgcg gtcactcgtg aaagacaccg ccgccgtttg tctgaggcct
     5941 tggctgcggt tgatgccgga cgacttgcct tgaacatcgc tccggaaatg gcgggcgatg
     6001 atctgcggcg tgcggcagat gccctgtcca gggtgactgg agccataggt gtagaagacg
     6061 tgctgggtga ggtcttttcc accttctgca tgggcaagtg aagcagcggt gttccacgtg
     6121 aaacgctctc gccggtagct ttttaggcct gttcgcccta tgtcgctgcc atgaactctc
     6181 cgacttcttt tgatgtgatc gtcatcggcg gcggccacgc aggctgtgag gctgcggcgg
     6241 cttcggcgcg tgcgggcgct cgtacccttc ttctgaccca gaagctggaa accgtcggtg
     6301 agatgtcttg caaccccgct atcggcggac tgggcaaggg ccatctggtt cgagagatcg
     6361 atgcactcga cggggtcatg gggcgcttag gcgatgtctc cggcatccag tttcgcctgt
     6421 tgaaccgctc caaaggcgcg gccgttcaag gtccccggag ccagatcgac cgccgccttt
     6481 accgtgaggc catgcaagcc gagttggccc aaaccccgaa cctgacgatg atagccggca
     6541 ccgccgaggg cctgatcatg gacggcgacc gggtggtcgg cgtcacgacg aactcgggcg
     6601 agacgatccg cgctgctgcc gtcgtcctca ccacgggcac cttcctgaac ggcgtcattc
     6661 atcggggcga cgagcgtatc cccgccgggc gtcacggtga ggagccatcc accggattgg
     6721 ccgccgacct ctacggcgcc ggcctccata tgggccgctt gaagaccgga acgccggctc
     6781 gcctcgacgg gcgaacgatc ggctgggacc ggctcgagat gcaggccgcg gatgatgagc
     6841 ctgtgccgtt cagcttcctg acggaacgga tcgaagttcc ccagatcgct tgcggcatca
     6901 cctcgaccaa cgaagagacg cacaagatca tcgccgccaa tctcggtgag agcgccgtct
     6961 acggtggcca tctcactgga cgcggtccgc gctattgtcc atccattgag gacaaggtcg
     7021 tgcggttcgc ggacaagacg tcgcaccagg tcttcctcga gccggaaggt ctggacgacc
     7081 cgaccgtcta tcctaacggt atctcgacct cggtgtcgcc ggagacccag ctggcgttcc
     7141 tgcgaacgat gcctgggctc gaggctgtcg aggtgttccg cttcggctac gccatcgaat
     7201 acgactatgt cgatccgcgt gagctgaccc cggcgctgga ggtcaaacgt cgcccgggct
     7261 tgtatctcgc cgggcaaatc aacggcacga ccggctatga ggaggccggg gcccaagggc
     7321 tgatcgcggg actgaacgcc gcgcgggctg ctgccggttc cgatccgatt atcctcggtc
     7381 gcgatcaggc ttacatcggc gtgatgatcg atgacctggt cacacgcggg gtcaccgagc
     7441 cgtatcggat gttcaccagc cgcgccgaat accggctgac cctgcgggcc gacaacgccg
     7501 accagcgact gacgccggtg ggcatcgcgg ccggaatcgt atcatctgac cggaccgcgc
     7561 ggttcggcgc caaggcggcg atgttggctg aggcggctac cctcgttcgt gagacacggt
     7621 acactccgaa ggaagcgcag gccctcggta ttgcagtcaa tgcagacggt cagcgccggt
     7681 ccatacggga gctgctgggg tatcccggtg ttgacttgac ggccttcgag acggtgcacc
     7741 ccatcgtctc gacatggtcg acggtcgtca gggagcaggt gcagatcgac gcgggctatg
     7801 ccggttatct ggatcggcaa agcgcagatg ccgacgcgct gagacgtgag gagcagttga
     7861 agcttcctgc caatctcgac tatgcgacga tcggcgggct gtccaatgag gtgcgtgaaa
     7921 agctgatgct gatccggcct cagacgctcg gacaggccgg gcgtatcgag ggcatgaccc
     7981 cgggagcgct gaccgctctg ctctcctatg tccgcaaggg cgtttcacgt gaaacggtcg
     8041 ccgcatgatg actcccgagg agttccagga cctgaccggg gcatcggcag aacggatggc
     8101 ggatcttgtc gcgttccacg ggcatttgac cgaagccaac gcggtgatga acctcgtggg
     8161 gccggggtct atgccggact tctggaaccg ccacgcctgg gactcggcgc agttgctcga
     8221 actggcgcct gacgcaaaga cgtgggcgga tctcggggca ggggcgggtt ttccaggtgt
     8281 cgttctggcc atcctgctga aggatgatcc ggatgctcac gtctggttgg tcgactcgct
     8341 ccagaagcgg tgcaagtttc tccagtatgt cgtcgatgaa ctggagcttc cagcgacggt
     8401 gatctggggt cgggcagagg agcaggagct ggaggtcgat atcgtcaccg cccgcgccgt
     8461 cgcttcgctc gacaagcttg tcggttatgc acagccctac ctctctcgcg gtgcacaagc
     8521 tttgtttctc aagggagaga aagctgagca ggagttgatt gatgcccgta aggtctggca
     8581 tctggatgca gagctctcga tctcccgcag cgatccgcgc ggccgtattg taaccgtcag
     8641 gaccctacgc cgtggcaaac gtttcgcctg aaaagaagac ccgcgtcctc gccgtctcca
     8701 accagaaggg tggggtgggc aagaccacga cggcgatcaa tctcggcact gccctggccg
     8761 ccatcggcga gaaggtcctg atcgtcgaca tggacccgca gggcaatgcg tccacgggtc
     8821 tcggtgttcc acgtgaaaca cgacgcatca cggtctacga cgtcatcgtc gacggccgtt
     8881 cggtccacga tgccgccgtc catacggccg tacccggcct tgacatcatc ccggcagacg
     8941 cggacatgtc cggcgtcgag atcgaactga gccaggccga ccgccgatcg tatcggttgc
     9001 gggatgcgct ggcggctcag ggcgatgctg gacataccgg gtatgactat gtgctgatcg
     9061 actgcccgcc gtcgctcaat ctcttgaccc tgaacgccat ggccgcagcg gacggcgtgc
     9121 tggttcctct tcagtgtgag ttcttcgcgc tcgagggtct gacgcagttg atgaaaacca
     9181 tcgagatggt gcgtcaatcg ttgaacccgt cgctggagat tcaaggtctc gttctgacca
     9241 tgtatgaccg ccgcagtgct ctgtcgggtc aggtggcggc tgacgttcgg agccatttcg
     9301 gcgagaaggt gtatgacgcg gtcataccga gaaacgtgcg ggtcgccgag gcgccgtcgt
     9361 tcggcaagcc ggtgctgatc tacgatctga aatgcacagg cagccaagcg tatctccgct
     9421 tggccaagga agtggtcggt cgtgagcgtc gtcgccgcgc tcagctcgcc gcctgataga
     9481 caaccaagaa acggaacaaa gacttgtctg aacgtcaaag aggattgggc cgcggccttg
     9541 cagccctgat gggcgaggac gccaatgagg ctgctccggc gacgggcggg cctgctacgg
     9601 gcgttcagac cgcgccgatc gagagcctga aacccaatcc ggaccagccg cgcaagatct
     9661 tcacgcagga gaatctggac gagctgacca actccattcg cgacaagggc atcctgcagc
     9721 cgatcctcgt gcgcacccag cccggccagg atggcatgtg gcagatcatc gccggtgaac
     9781 gccgttggcg cgctgcgcag gccgctcgcc tggccgtggt gccgatcgtc gtgcgtgag
//
LOCUS       DASVTM010000106         7036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_435901,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000106 DASVTM010000000
VERSION     DASVTM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435901"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..343
                     /locus_tag="VF633_03070"
     CDS             77..343
                     /locus_tag="VF633_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470071.1"
                     /translation="MKPAVIAIASLALVVAVGGCTDSKRASRDATWGDKPADITCWTY
                     GTQSFSGRSTGKVEYDEGDRISFVDAANQRYTTVDGDCRVVYSK"
     gene            complement(347..571)
                     /locus_tag="VF633_03075"
     CDS             complement(347..571)
                     /locus_tag="VF633_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470072.1"
                     /translation="MSSSMPKANDTVHLRLSMRNDGGPFWQTNAVIASVAGRSVVLDG
                     FDRQVTASATELKPAGAGRWTLDWEIRTRP"
     gene            complement(636..1721)
                     /locus_tag="VF633_03080"
     CDS             complement(636..1721)
                     /locus_tag="VF633_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8470073.1"
                     /translation="MALFTRKAPAAVEATAEASSPDGKRPISLALQGGGAHGAFQWGV
                     LDRLLEDDRLDIRAVTAASAGAMNGAALVSGLATGGAEGARKSLDKLWRETNQSGGRN
                     VFGDSSIWSSAMTPNWLTETTAWRSMETLAGSMSPYQFNPFNLNPLKRVLDATIDFDA
                     VRASDIKLFVAATAVRHAKARIFTTAEITDQVLLASACLPHLFQAVEIEGEPYWDGGY
                     LANPPRWPLFYADTPDDILLMTLNPFEREEAPRAAGEIMDRLNEVVFNAPLAAELRAV
                     AFVQELLEQGMLKEDARGRYRHILMHAIEADGWLGDQTLGSKFNTEWTYLNSLKARGC
                     QAAEEWLATCFNQVGVSSSVDLQARFG"
     gene            complement(1740..2570)
                     /locus_tag="VF633_03085"
     CDS             complement(1740..2570)
                     /locus_tag="VF633_03085"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX8470074.1"
                     /translation="MDNPRDHERPVYRDPNDLKGQVAVISGSTSGIGLALARAVAARG
                     GDVVLNGLGDPAEIERTRAEMEASCGVRVRYHAANMLRGDEVADMVAFGKHQLGRVDI
                     LVNNAGIQHVESVEKFPSDQWEKIIAINLSSTFYATRAAIPIMKAQGRGRIVNIASAH
                     GLVASPFKSAYVAAKHGVLGFTKTVALELAQENITCNAICPGFVETPLVAAQIADQAR
                     LNGVSKEVALRDIILEAQPTKRFVTTDELTGIFLYLVSDLGASATGASFSVDGGWTAK
                     "
     gene            complement(2673..3446)
                     /gene="yaaA"
                     /locus_tag="VF633_03090"
     CDS             complement(2673..3446)
                     /gene="yaaA"
                     /locus_tag="VF633_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEX8470075.1"
                     /translation="MLIVLSPAKRLDFTETDPELPATAPRFVEDIASLSRTAKRQTKA
                     DLRKLMSISEDLAKLNQQRFKAFDPEATDVGTQAVMAFAGDVYQGLDARSLDAKSLDW
                     AQSHLRILSGLYGLLRPLDRIQPYRLEMGVRLKTRRGNSLYDFWDDRISKQLNIDAEG
                     QPHPVLVNLASQEYFGAVDAKALKIPVVTPNFRETKDGESRIISFFAKKARGTMARFA
                     IDERIENPTDLKTFDRDGYTFDKSLSSETDWIFTRSGNS"
     gene            complement(3568..4974)
                     /locus_tag="VF633_03095"
     CDS             complement(3568..4974)
                     /locus_tag="VF633_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8470076.1"
                     /translation="MNAPSPAAIDELKAALGPSGWTQDPFTIAPHLTEWRGRWSGETP
                     ILLTPGSTEDVRKAVAICASHGVAVIPQGGNTGLVGGQIPFGEVLLSTRRLRTVRDVT
                     PLDDAMTLEAGITLLEAQQHAAAADRFFPLSLAAEGTATVGGVISTNAGGTQVLRYGM
                     MRDQVLGLEAVMPNGEIFHGLKRLRKDNTGYDLKQLLIGAEGTLGVVTAATLKLYPVM
                     RSRAVAMVALETAAASIELLARARAETGGGVEAFELMKRLGAELVVKNIPDSRWPLEP
                     IPEWSVLIEIASGTPGGAEAQMERLLEVAFEEGLIVDAAIAQNDAQRKAFWRLREEHS
                     AALKPEGGGWKHDVSVPISRIAHFIDEATAAVERFHPGARVSVFGHIGDGNLHYDVLP
                     GGGEDVPAFIARWKEGSKVVHDIVARYDGSISAEHGLGRLKTEEVRRYKSPLEIETMA
                     AIRRAIDPQRIMNPAVLF"
     gene            5031..5462
                     /locus_tag="VF633_03100"
     CDS             5031..5462
                     /locus_tag="VF633_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8470077.1"
                     /translation="MDFAPDLPIPAPVPAVGVVCLRGNEVLLIRRGKPPRQGEWSLPG
                     GRIEPGERAVDAALRELREETGVEAEIVGLLDVVDGFFPEAGLHYVLIDYVATWCSGE
                     PVAGDDAADARFVRRAEVDALIAWNETRRIIAKAFENMNTV"
     gene            5612..5959
                     /locus_tag="VF633_03105"
     CDS             5612..5959
                     /locus_tag="VF633_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470078.1"
                     /translation="MIKLMKVALAAALLSAAAPVAAMAQTTEPVTFTLNNQTDHVLTA
                     LYISKVSTNEWEEDIFGADVLGAGDSVEVTIDDNLPDCEYDLKAVFEDGDEAILGSEN
                     FCELDGGEVNVTE"
     gene            6117..>7036
                     /locus_tag="VF633_03110"
     CDS             6117..>7036
                     /locus_tag="VF633_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8470079.1"
                     /translation="MSYVAITLVVIFAFVLLFSVIKIVPQGREFTVERFGRYTKTLKP
                     GISILTPFVERVGRRMNMMEQVLDVPQQEVITKDNAMVKVDAIVFIQVMEASAAAYRI
                     ENLPYAITQLCMTNLRTVVGSMELDEVLFQRDSINTRLLTVIDAATEPWGVKVNRIEI
                     KDLTPPADITNAMARQMKAEREKRAIITEAEGEKQAAIARAEGAKQSAILQSEGRKEA
                     AFRDAEARERAAAAEAKATELVSNAIAAGDVNAINYFVAQKYVEAFAELARNPTAKTV
                     IVPAEMGSLVGAIAGIGELVGLAKDQQQVAR"
BASE COUNT         1247 a         2221 c         2317 g         1251 t
ORIGIN      
        1 ggtatgtggg gtagggcgga ttgtcgcatc ccaacgttgg gtgaaggcgt tatcgtccgc
       61 tgcacaggag cctcccatga aacccgccgt catcgccatc gcctcactcg ccctcgtagt
      121 cgcagtcggc ggctgtacgg acagcaagcg cgccagccgc gatgcgacct ggggcgacaa
      181 gccggcggac atcacctgct ggacctatgg tacgcagagc ttcagcggtc gctctaccgg
      241 caaggtcgag tacgacgagg gcgaccggat ctcgttcgtg gacgccgcga accagcgcta
      301 cacgaccgtc gatggcgact gccgggtggt ctactccaag taggcgtcag ggccgggtgc
      361 ggatttccca atccagggtc cagcgaccgg cgcccgcagg cttcagttcg gtggcagagg
      421 cggtgacctg acggtcgaag ccgtccagca cgaccgagcg accggcgacc gaggcaatga
      481 cggcgttggt ctgccagaac gggccgccgt cgtttcgcat cgacagacgc aggtggacgg
      541 tgtcgttggc cttgggcatc gaggaagaca tcggcgggtc cttggggagg cgcggcgatg
      601 tgcgcccgcg ggcagtcgtc gtcaaggatg tcatttcagc cgaaccgggc ctgcagatcg
      661 acgctggaac tgaccccgac ctgattgaag caggtcgcca gccactcctc ggccgcctga
      721 caaccacggg ccttcaggct gttgagatag gtccattcgg tgttgaactt ggagcccagc
      781 gtctgatcgc cgagccagcc gtcggcctcg atcgcgtgca tcaggatgtg ccgatagcga
      841 ccgcgcgcat cctccttcag catgccttgc tcaagcagtt cctgcacgaa ggcgacggcc
      901 cgcagttcgg cggccagagg ggcgttgaag accacctcgt tgagccggtc catgatctcg
      961 ccggccgcgc gtggggcctc ctcacgctcg aacgggttaa gcgtcatcag caggatgtcg
     1021 tcgggcgtgt cggcatagaa cagaggccac cgcggcgggt tggccagata gcccccgtcc
     1081 caatagggct cgccctcgat ctccaccgcc tgaaacagat gcggcaggca ggccgaggcc
     1141 agcaacacct gatcggtgat ctcggccgtg gtgaagattc gcgccttggc atggcggaca
     1201 gccgtcgcgg cgacgaacag cttgatgtcg gacgcccgca ccgcgtcgaa gtcgatcgtc
     1261 gcgtcgagca cgcgcttgag cggattgagg ttgaacgggt tgaactgata cgggctcatc
     1321 gaccccgcca gcgtctccat cgaccgccag gccgtcgtct cggtcagcca gttgggagtc
     1381 atggccgacg accagattga actgtcgccg aagacgttgc ggccgcctga ctgattagtc
     1441 tctcgccaca gcttgtcgag cgacttgcgc gcgccctcag caccgccggt agccagtccc
     1501 gagaccagcg ccgcaccgtt catcgcgcct gctgacgcgg ccgtgacggc gcgaatgtcg
     1561 agccggtcat cctccagcaa ccggtccagc acgccccact ggaacgcgcc gtgcgcccca
     1621 ccgccctgaa gcgccagcga gatgggacgt ttgccgtccg gggacgatgc ctccgccgtg
     1681 gcttcgactg ctgcaggagc cttacgggtg aacagcgcca tcggccctcg gggttgctgc
     1741 tacttggcgg tccagccgcc gtcgacggag aagctggcgc cggtggccga ggcgccgagg
     1801 tcggacacca gatagaggaa gattccggtc agttcgtcgg tcgtgacgaa ccgcttggtc
     1861 ggctgggcct cgaggatgat gtcacgcagg gcgacctcct tcgaaacgcc gttcagccgg
     1921 gcctgatcgg cgatctgggc cgcgaccagc ggcgtctcca cgaagccggg gcagatggcg
     1981 ttgcaggtga tgttctcctg cgccagctcc agcgccaccg tcttggtgaa gccgagcacg
     2041 ccatgcttgg cggcgacgta ggccgacttg aacggcgagg cgaccagccc gtgcgccgag
     2101 gcgatgttga cgatccgccc gcgcccttgc gccttcatga tcgggatcgc cgcccgcgtc
     2161 gcatagaagg tcgacgacag gttgatggcg atgatcttct cccactggtc cgacgggaat
     2221 ttctcgaccg actcgacatg ctggatgccg gcgttgttga ccaggatgtc gacgcgaccg
     2281 agctggtgct tgccgaacgc gaccatgtcg gccacctcgt cgccgcgcag catgttggcg
     2341 gcatggtagc ggacgcggac gccgcagctg gcttccatct cggcgcgggt gcgttcgatc
     2401 tcggccggat cgcccagacc gttcaggaca acgtcgccgc cgcgcgccgc gactgcccgg
     2461 gccagcgcca gaccgatgcc cgaggtcgac cctgagatga ccgccacctg acccttgaga
     2521 tcgttgggat cccggtagac gggacgctcg tggtccctgg ggttatccat cgcgggggct
     2581 ttggtccggt tgaacatgtc ggcgtttccg aaggcgtggt ttctgtttgg acgcagacta
     2641 gtcgctcggg gcggcgatgg cgacagcagc gatcacgaat ttcctgatcg ggtgaatatc
     2701 cagtccgtct cactcgaaag cgacttgtcg aacgtgtagc cgtcgcggtc gaaggtcttg
     2761 aggtcggtgg ggttctcgat ccgctcatcg atggcgaacc gggccatggt tccacgcgcc
     2821 ttcttggcga agaaggagat gatccggctc tcgccgtcct tcgtctcgcg gaagttcggg
     2881 gtgacgacgg ggatcttcag cgccttggcg tcgaccgcgc cgaaatactc ctggctggcc
     2941 aggttcacga gcacggggtg cggctgccct tcggcgtcga tgttcaactg cttcgagatc
     3001 cggtcgtccc agaagtcgta aaggctgttc ccgcgccggg tcttcagccg gacgcccatt
     3061 tccagccgat agggctggat ccggtcgagt ggacgcaaca gaccgtagag acccgacagg
     3121 atacgcagat gagactgcgc ccagtcgagc gacttggcat ctagcgaacg ggcatccaac
     3181 ccctgataga catcgcccgc gaaggccatc acggcctggg taccgacatc ggtcgcctcg
     3241 gggtcgaagg ccttgaaccg ttgctggttc agcttggcca gatcctcgga gatgctcatc
     3301 agcttgcgca ggtcagcctt tgtctggcgc ttggccgtgc gtgacaggct ggcgatatcc
     3361 tcgacgaagc ggggcgcagt ggccggcagt tccgggtcgg tctcggtgaa atccagccgc
     3421 ttggccgggg acagcacgat cagcacggca cactcatcat caggttcgtc atgggcgtct
     3481 ccggtctcaa gaggcgcgat ctaggccggt tccgtgacga attgaaccgt cgcgggcgaa
     3541 ctgtcctcag cgttcgatcc gggagggcta aaacagaacc gcagggttca tgatccgctg
     3601 agggtcgatg gcacggcgga ttgcggccat ggtctcgatc tccagcggcg atttgtagcg
     3661 gcggacttcc tcggttttga gccgccccag accgtgttcg gcggagatcg agccgtcgta
     3721 gcgggcgacg atgtcgtgga cgaccttgga gccttctttc cagcgcgcga tgaaggcggg
     3781 aacgtcttcg ccgccgccgg gcaacacatc gtagtgcagg ttgccgtcgc cgatgtggcc
     3841 gaagaccgag acgcgggcgc ccggatggaa gcgttcgacg gcggcggttg cttcgtcgat
     3901 gaagtgggcg atccggctga tcgggacgga gacgtcgtgc ttccagccgc cgccttcggg
     3961 tttcagcgcc gcagaatgtt cctcgcgcag acgccagaag gctttgcgct gggcgtcgtt
     4021 ctgggcgatg gcggcgtcga cgatcaggcc ttcctcgaag gcgacctcga gcaaccgttc
     4081 catctgggct tcagcgccgc cgggagtacc tgaggcgatt tcgatcagca ccgaccattc
     4141 ggggatgggt tcgagcggcc aacgggagtc ggggatgttc ttgaccacca gttctgcccc
     4201 cagacgcttc atcagttcga aagcctcgac accaccaccg gtttcggctc gagcccgggc
     4261 cagaagctcg atggatgccg ccgctgtctc aagggcgacc atggccacgg cccgactgcg
     4321 cataacggga tagagcttca gcgtcgccgc tgtaaccacg ccaagggtgc cttcagcccc
     4381 gatcagaagt tgcttgaggt catagccggt gttgtccttg cgcagccgtt tcaggccgtg
     4441 aaagatctcg ccattcggca tcacggcctc gaggccgagc acctgatcgc gcatcatgcc
     4501 gtagcgaaga acctgcgtgc ctccggcgtt ggtagagatc acgcccccga ccgtcgccgt
     4561 gccctccgca gcaaggctga gggggaaaaa gcgatcggca gccgccgcat gctgctgggc
     4621 ctccagcagg gtaatgccgg cctccagcgt catcgcatca tcgaggggtg tgacatcgcg
     4681 caccgttctc agtcgccgcg tcgaaagcag gacttcgccg aaggggatct gtcccccgac
     4741 gagaccggtg ttgccgccct gcgggatgac ggcgacgccg tggctggcgc aaatcgccac
     4801 ggccttgcgc acgtcttcgg tggagcccgg cgtaagcaag atgggagtct cgccgctcca
     4861 acgaccccgc cactcggtca agtgaggcgc gatggtgaag ggatcctggg tccagccgga
     4921 cggtccgagc gcggccttga gctcatcgat tgcggcgggg gagggcgcgt tcatggacgc
     4981 tcttctgtca aaaacgtgca caggacgaaa gtcgaaagct atgctggggg atggattttg
     5041 cgccagacct tccgattccc gcccccgtgc ctgccgtcgg cgtggtctgt ctgcgcggga
     5101 atgaggtgct gttgatccgt cgcggcaagc cgccgcgcca aggcgaatgg tcgctgccgg
     5161 gcggccggat cgagcccggc gaacgcgccg tcgacgccgc tctgcgcgaa ctgcgggaag
     5221 aaacgggtgt ggaggcggaa atcgtcggcc ttctcgacgt cgttgacggc ttctttcccg
     5281 aagcgggtct gcattacgtc ttgatcgact acgtcgcgac atggtgctcc ggcgaaccgg
     5341 tggcgggcga cgacgccgcc gatgcccgtt tcgtcaggcg ggctgaagtc gacgcgctga
     5401 tcgcctggaa tgagacccgc cgcatcatcg cgaaggcgtt cgaaaacatg aatacggtgt
     5461 gaatgaatcg accctaaggc gttgtttccg catgattgca gcgaattatg aacgtgacag
     5521 catggcagta aagcgcaata agccctcggc tcggcaaccg ccgggtctgg gggctatccg
     5581 ccggcgtgag ccggtattgg gggactatac tatgatcaaa ctgatgaagg tggctcttgc
     5641 cgccgccctg ctcagcgctg ctgcgcccgt cgctgcgatg gcgcagacca ccgagccggt
     5701 tacgttcacg ctgaacaacc agaccgacca cgtgctgacg gcgctttaca tctccaaggt
     5761 ctccaccaac gagtgggaag aagacatctt cggcgcagac gtcctgggtg ctggcgacag
     5821 cgtcgaagtc accatcgacg acaacctgcc agactgcgaa tacgacctga aggccgtgtt
     5881 cgaagacggc gacgaagcca tcctgggctc ggaaaacttc tgcgaactgg acggcggcga
     5941 agtcaacgtc accgaatagt cctcgcgacg ttcgttacgg atcggggcgg tctctgctga
     6001 ggccgccctt tttcgtttcg ggccgccttc aggttgcgaa aacagccggg acagcgtccg
     6061 gtttccatgg cggggcttat ggcgacggtc tagactggct ccaagggaga ctgttcatga
     6121 gctatgtcgc catcaccctc gtggtgatct tcgcctttgt cctgctgttc agcgtgatca
     6181 agatcgtgcc gcagggtcgc gagtttacgg tcgagcgctt cggccgctac accaagacct
     6241 tgaagcccgg catcagcatc ctgaccccgt tcgtggagcg cgtcggacgc cgcatgaata
     6301 tgatggagca ggtcctcgac gtgccccagc aggaggtcat caccaaggac aacgccatgg
     6361 tgaaggtcga cgccatcgtc ttcatccagg tgatggaggc ctcggccgcc gcctatcgga
     6421 ttgagaacct gccttacgcc atcacccaat tgtgcatgac caacctgcgc accgtggtcg
     6481 gttcgatgga gctggacgag gtgttgttcc agcgcgactc gatcaatacc cggctgctga
     6541 ccgtcatcga cgcggctacc gagccgtggg gcgtcaaggt caaccgcatc gagatcaagg
     6601 acctgacccc gcctgctgac atcaccaacg ccatggctcg tcagatgaag gccgaacgcg
     6661 aaaagcgcgc catcattacc gaggccgagg gcgagaaaca ggccgccatc gcgcgcgccg
     6721 agggcgccaa acaatcggct atccttcagt ccgaaggccg caaggaagcc gccttccgtg
     6781 acgccgaggc ccgtgaacgc gccgccgccg ccgaggccaa ggcgaccgaa ctggtgtcga
     6841 acgccatcgc cgctggcgac gtcaacgcca tcaactactt cgtcgcccag aaatacgtcg
     6901 aggccttcgc cgaactggcg cgcaacccga ccgccaagac cgtgatcgtt cccgccgaga
     6961 tgggcagcct ggtcggcgcc atcgccggca tcggcgaact ggtcggtctg gcgaaggatc
     7021 agcagcaggt cgcgcg
//
LOCUS       DASVTM010000107         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_809402,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000107 DASVTM010000000
VERSION     DASVTM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809402"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VF633_03115"
     CDS             <1..746
                     /locus_tag="VF633_03115"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX8470080.1"
                     /translation="AGELSLQLRRRGLSVEHKANHSPVSNADLALDALLTDRLRAARP
                     DYGWLSEETADTSERLSKERIFVVDPIDGTVAYMKNKPWWCVPIAVVENGHPVAAVIH
                     VPSLNETFEATLGGGARLNGRAISPSDTDDLDDASVLADAKLMERPEWPEPWPPMRYE
                     KRNALAYRMALVAAGMFDAAIAMGPKWDWDVAAGQLIATEAGAKVTDHLGRPWLFNRP
                     DPRQTSLVCSAPALHPLIIRRTAPIPLAL"
     gene            774..1019
                     /locus_tag="VF633_03120"
     CDS             774..1019
                     /locus_tag="VF633_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX8470081.1"
                     /translation="MSVHTEVETPQLLHLVIGGELRHLDAPVFRDLSKVEFVGAFGSY
                     DEAKKAWKARAQATVDNALMRFFILHAHKMIDPRADH"
     gene            1367..1639
                     /locus_tag="VF633_03125"
     CDS             1367..1639
                     /locus_tag="VF633_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470082.1"
                     /translation="MTLFGTTLGTEQIVGLIGLLVLLVFWVVALRNEQGALFHLRRWE
                     ADRKARREAELMRENGGPVAPPSSDTPSSRKPAPTRPDDPKRGPWS"
     gene            complement(1657..2322)
                     /locus_tag="VF633_03130"
     CDS             complement(1657..2322)
                     /locus_tag="VF633_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX8470083.1"
                     /translation="MPRFDLTSTAGRFHAGWDYFWSDHAFLRLAFSNAHWLGPDLVRT
                     NQPSPRQLKGWADRGIKTVINLRGERDESYYWLEKAACEALGLTLIDAPLDSRDVPEP
                     YRIHRAIDLFRTVEYPVLIHCKSGADRAGLMAVLYRHFHLGEPIPVAMEQLSKKYLHS
                     REGLTGVLDYFLEKYVREIEPTGMTFLEWVDSPDYDPKAIRAEFKAGWWGTLLTDKLL
                     RRE"
     gene            2464..>2943
                     /locus_tag="VF633_03135"
     CDS             2464..>2943
                     /locus_tag="VF633_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8470084.1"
                     /translation="MTTTGVSDEDEGLRPLLARIWRDYLSRHKRALFASILCAIIAGG
                     LNAAILKLLEPAINGLFVDHSVPVKLWGLIPVSADEALFWIPTAIVMVAFVWTLASLG
                     QAALVNRLGHGIVGDIQVRLFGAMIRADLARLRSQHSGSFVSSVLFDANLVREAFTNG
                     "
BASE COUNT          501 a          988 c          941 g          513 t
ORIGIN      
        1 aggcgggcga actgtcgctg cagctgcggc gtcggggcct gagcgtcgag cacaaggcga
       61 accattcgcc ggtcagcaac gccgacttgg cgctggacgc gctgttgacc gatcggctgc
      121 gcgccgcgcg acccgactac ggctggctgt ctgaagagac ggcggacacc agcgagcgtc
      181 tgtcgaagga gcgcatcttc gtcgtcgatc cgatcgacgg caccgtcgcc tatatgaaga
      241 acaagccgtg gtggtgcgtt ccgatcgccg tggtcgagaa cggtcatccg gtggcggcgg
      301 tgattcacgt gccgtcgctg aacgagacgt tcgaggcgac gctgggcggc ggcgcgcgac
      361 tgaacggacg agcgatctcg ccgtccgaca ccgacgacct cgacgacgcc tcggtgctgg
      421 ccgacgccaa gctgatggag cggcccgagt ggcccgagcc ctggccgccg atgcgctacg
      481 agaagcgcaa tgcgttggcc tatcgcatgg cgctggtcgc tgcggggatg ttcgacgcgg
      541 ccatcgccat gggtccgaag tgggactggg acgtcgctgc cggacaactg atcgccaccg
      601 aggcgggcgc aaaggtcacg gatcacctcg gtcgaccgtg gctgttcaat cgtccggacc
      661 cgcgtcaaac cagcctggtt tgcagcgcac cggccctgca cccgttgatc atccggcgca
      721 cagcgcctat cccgctggcc ttataagccc tcccccgctt tcaaggacct gagatgagcg
      781 ttcataccga agtcgaaacc ccgcaactgc tgcacctcgt catcggcggc gagctgcgcc
      841 acctcgacgc acccgtcttc cgcgacctgt ccaaggtcga gttcgtcggc gccttcggca
      901 gctatgacga agccaaaaag gcgtggaagg cccgcgcgca ggccacggtc gacaacgccc
      961 tgatgcggtt cttcatcctg cacgcccaca agatgatcga tccgcgcgcg gaccattagg
     1021 ccgcaaggtt aactgcggaa cacgccccct cgcttacgcg cttcatgccg tgagcctccg
     1081 ctttgatgag ctgggcgttc ctgcaggagt attccatggt tccttttctc gtcgtctccg
     1141 gcctgttgtt gctgggcgcg gcgatcgtcg cctgggcgac ccggcccacg gcgtcttacc
     1201 ccgccgtgca aatgctccgg ggcaaacaag gacaagccag ggggcgcgag gcgccaggcg
     1261 atcggcgcac gaagccgaag ttgcccgccc agcccgaggc gtttgccagc gcgcctgtct
     1321 cggcgccgcc caccatggcg agtgctgcat cacgagaggc gaggccatga ccctgtttgg
     1381 aacgacgctc ggcactgaac agatcgttgg actgatcggt cttctggtcc tgctggtctt
     1441 ctgggtcgtg gcccttcgca acgaacaggg cgccctgttc cacctccgca gatgggaggc
     1501 cgatcgcaag gccagacgcg aggcggagct gatgcgcgag aacggcggac cggtcgcccc
     1561 gccatcatcc gacacgccgt catcccgcaa gcctgcccct acccggccgg atgaccccaa
     1621 gcgcggcccc tggagttaat ccggacaggc tccgccctac tcccggcgca gcagtttgtc
     1681 tgttagcagc gtgccccacc agccggcctt gaactcggcg cggatcgcct tgggatcata
     1741 gtcgggactg tcgacccatt cgaggaaggt catccctgtc ggctcgatct cgcggacgta
     1801 tttctcgagg aaatagtcga gcacgccggt caatccctcg cggctgtgca gatacttctt
     1861 cgacaactgc tccatggcga ccgggatcgg ctcgccaaga tggaaatgcc ggtacagcac
     1921 cgccatcagc ccggcccgat ctgcgcccga cttgcagtgg atcaggaccg ggtattcgac
     1981 ggtcctgaac aggtcgatgg cgcggtggat gcggtacggc tcaggcacgt ctcgcgagtc
     2041 cagcggcgca tcgatcaggg tcagaccgag cgcctcgcac gccgccttct ccagccagta
     2101 atagctctcg tcgcgttcgc cgcgcaggtt gatgaccgtc ttgatccccc ggtccgccca
     2161 gcccttcaac tgccggggcg atggctggtt ggtgcgcacc agatcgggcc ccagccaatg
     2221 ggcgttcgag aacgccagcc tgaggaaggc atggtcgctc cagaaatagt cccatccggc
     2281 atgaaaccgg ccggcggtgc tggtgaggtc gaaacgcggc atagggcgca gatagaggtt
     2341 cgaggccgcc gcgtcatccg cacacgtgac gatacaatcc cgccgacgca tcgaaggtca
     2401 cagttcgctc ggaataagcc cacgcgggtt gacttgccgg gccgtccgtc cgacctccgt
     2461 ccaatgacga cgactggcgt ttccgacgaa gacgaaggcc tgcgcccgct gctggcccgg
     2521 atctggcgcg actacctctc gcggcacaag agagcgctgt tcgcctcgat cctgtgcgcc
     2581 atcatcgcag gcggactgaa cgccgcgatc cttaagctgc tggaacctgc aatcaacggc
     2641 ctgttcgtag atcacagcgt gccggtgaaa ctgtgggggt tgatccccgt ttcggcggac
     2701 gaggccctgt tctggatccc gacggcgatc gtcatggtcg ccttcgtctg gaccctggcg
     2761 tcgctgggtc aggcggcgct ggtcaacagg ctgggccacg gcatcgtcgg tgatattcag
     2821 gtgcggctgt tcggagccat gatccgcgcc gacctcgccc gtctgcgcag ccagcattcc
     2881 ggcagcttcg tctcctcggt gctgttcgac gccaatctgg tgcgcgaggc cttcaccaac
     2941 ggc
//
LOCUS       DASVTM010000108         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_436048,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000108 DASVTM010000000
VERSION     DASVTM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436048"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1392
                     /gene="fabF"
                     /locus_tag="VF633_03140"
     CDS             112..1392
                     /gene="fabF"
                     /locus_tag="VF633_03140"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX8470085.1"
                     /translation="MRRVVVTGLGLVTPLGWGVEHTWQGIVEGRSGIGPITTFDTEGY
                     GCTIAGEVPSVDGRGQGGPDVPGSFDPDKVMSAKDRKRVDDFILFGIAAADEALNDAN
                     WHPESDEDQERTGVMMGSGIGGLGPIAETALILDKQGPRRVSPFFIPSSLINLTSGQI
                     SIRHGLKGPNHAVVTACASGVHAIGDAARMIAFDDADVMVAGGAESSIVPIGIAGFLA
                     CKALCTAYNDTPETASRPYDRGHAGFVMGEGAGVVILEEYEHAKARGAKIYAEVIGYG
                     MSGDAYHITAPSEDGDGAYRAMKAAVKRAGIAIEDIDYVNAHGTSTMADWIELRAVER
                     LVGDHAKNLAMSSTKSMTGHLLGAAGAIEAVFSVLAIRDQIAPPTINLDDPEHQTDID
                     LVPHKGKPMEIDVAMSNSFGFGGTNASIIFKKVA"
     gene            1392..2621
                     /gene="mltG"
                     /locus_tag="VF633_03145"
     CDS             1392..2621
                     /gene="mltG"
                     /locus_tag="VF633_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX8470086.1"
                     /translation="MLKGSGGPIKGPVKGPRPVARGKRSGKMAALLAASATFSLFLIA
                     ALAWAWSVYYAPGPSARMGDSTVVTLPSGSGVSAIAATLKNAGVIRSTDMFKAAATLT
                     GADRKLRAGEYQVPTRASLKNVLRLLTDGRVVRHYVTIPEGWSSAQAIDILNREQVLT
                     GAVETTPAEGSLWPDTYEVARGETRAAVVARMQRAMTENLAKLWAARGQGLVVRTPQE
                     ALTLASIVEKETGLAEERPRVAAVFSNRLRMGVRLESDPTIVYGITRGRPLGRGIRQS
                     ELQANTGYNTYLIDGLPPTPIANPGKDAIAAVLNPPRSTDLFFVADGTGGHVFASTYE
                     QHLVNVARWREIERRKAGLPPGATPPAAPGALAPGTAPTDQPLTGEAVITMQPPGAIT
                     PSRAAPPVTPPPATTPR"
     gene            2618..3502
                     /locus_tag="VF633_03150"
     CDS             2618..3502
                     /locus_tag="VF633_03150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX8470087.1"
                     /translation="MSGISGMTGFGRADGALDGWSWSVEARSVNGRNLEVRFRGPNGF
                     DDLERLTKAAAQARLNRGQVTVGVQAKQAQGSAGALKVNEAVLARYLELANQLAEEGA
                     TPPSADGLLGLRGVLDVADDDIDAETRTPIEAAMAVSVGEALDALKVSREREGAQLTP
                     VIADFIGRIEALIGKAEGEASAQTDHIRERFGRRMAELAPDATGLEDRIVVEAAALAT
                     RADVREELDRLTAHVASARTLLLQPPAGRKLDFLMQEFMREANTLCSKSATTPLTAIG
                     LDLKAVIEQLREQIQNVE"
     gene            3492..>3834
                     /locus_tag="VF633_03155"
     CDS             3492..>3834
                     /locus_tag="VF633_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX8470088.1"
                     /translation="MSNDRTPRRGVLLIVASPSGAGKTSLCRRLMADHGGLELSVSMT
                     TRGIRPGEVADRDYHFVTPEAFQALIDQDAFLEWADVHGSRYGSPRAPIDKALEEGRD
                     VLFDIDWQGAEQ"
BASE COUNT          675 a         1250 c         1304 g          605 t
ORIGIN      
        1 tggtcattct cgttagcacc ctgacgagga gatcagcgct cgccctagcg agtcatcgtg
       61 gcgcccacta tgctgtgctg cttgagtctc aaccatcagg gagcgcagcc tatgcgccgc
      121 gtcgtcgtca ccggcctcgg actggtcact cccctgggct ggggcgtaga acacacatgg
      181 caagggatcg tcgaagggcg ctcgggcatc ggcccgatca cgaccttcga cactgaaggc
      241 tatggctgca ccatcgcggg tgaagtgcct agcgtggacg gacgggggca gggcgggccg
      301 gacgttcccg gcagcttcga tcccgacaag gtgatgtcag ccaaggaccg caagcgtgtc
      361 gacgacttca tcctgttcgg catcgccgcc gccgatgagg cgctgaacga cgcgaactgg
      421 catcccgagt ccgacgagga tcaggaacgc accggcgtaa tgatgggctc cggcatcggc
      481 ggtctcgggc cgatcgccga gaccgcgctg attctcgaca agcagggccc gcgtcgcgtc
      541 agtcccttct tcatcccttc gtccctgatc aacctgacgt cgggccagat ctcgatccgg
      601 catgggctca agggtccgaa ccatgcggtg gtcacggcct gcgcctccgg ggtccacgcc
      661 atcggcgacg cggcgcgcat gatcgccttc gacgatgccg acgtgatggt cgcgggcggg
      721 gccgaaagct cgatcgtgcc gatcggcatc gccggcttcc ttgcctgcaa ggcgctgtgc
      781 accgcctaca atgatacgcc ggagacggcc tcgcgtccct atgaccgagg gcatgcgggc
      841 ttcgtgatgg gcgagggcgc gggggtcgta attctcgagg aatacgagca cgccaaggcc
      901 cgtggggcca agatctacgc tgaggtgatc ggttatggaa tgtcgggcga cgcctaccac
      961 ataaccgccc cgtccgagga cggcgacggc gcctatcgcg ccatgaaggc ggcggtgaag
     1021 cgggccggga tcgccatcga ggacatcgac tacgtcaatg cccacggcac atcgacgatg
     1081 gccgactgga tcgaactgcg cgcagtcgag cggctggttg gcgaccacgc caagaacttg
     1141 gccatgtcct cgaccaagtc gatgaccggg cacctgctgg gcgcggcggg cgctatcgag
     1201 gcggtgttct cggtgctggc catccgcgac cagatcgcgc ctccgacgat caaccttgac
     1261 gatcccgaac accaaaccga catcgacctc gtgccacaca agggcaagcc gatggagatc
     1321 gacgtggcga tgtccaacag cttcgggttc ggtggcacca acgcctcgat catcttcaag
     1381 aaggttgcct gatgctcaaa gggtccggcg gtcctatcaa gggaccggtg aaagggcccc
     1441 ggcccgtcgc gcgcggcaaa cgatcgggca agatggcggc gttgctggcg gcctcggcca
     1501 ccttcagcct gttcctgatc gccgccttgg cctgggcctg gagcgtctac tacgcgcccg
     1561 gaccatcggc gcggatgggc gattcaacgg tcgtcacgct cccgagcggg tcgggcgtct
     1621 cagccatagc ggcgacgctg aagaacgcgg gtgtcatccg ttcgacggac atgttcaagg
     1681 ccgcagcgac cctgaccggc gccgaccgca agttgcgggc gggggagtat caggtgccga
     1741 ccagggcctc gctgaagaac gtgctgaggc tgctgacaga cgggagggtc gtgcgccatt
     1801 atgtgaccat ccccgagggc tggtcgtccg cgcaggccat cgacatcctc aaccgcgaac
     1861 aggtgctgac cggtgcggtc gagacgacgc ccgccgaggg ctcgctgtgg cccgacacct
     1921 atgaggtcgc acgtggtgag acccgcgccg ccgtggtcgc ccgcatgcaa cgggccatga
     1981 ccgagaacct cgccaagctt tgggccgcgc gcggtcaggg gcttgtcgtg cggacgccgc
     2041 aggaagccct gaccctggcc tccatcgtcg agaaggaaac cggtctggcc gaggaacgtc
     2101 cgcgggtggc ggcggtgttc tcgaaccgcc ttcgcatggg cgtgcggctc gagagcgatc
     2161 cgaccatcgt ctacggcatt acccgcggac gaccgctggg acgggggatc cggcagtctg
     2221 aactgcaggc caacaccggc tacaacacct atctgatcga cggcctgccg ccgacgccga
     2281 tcgccaatcc gggcaaggac gcgattgccg ccgtgctcaa cccgccgcgt tcgactgatc
     2341 tgttcttcgt ggccgacggc acaggcggac acgtcttcgc cagcacctat gagcagcatc
     2401 tcgtcaacgt cgcacgatgg cgcgagatcg agcggcgcaa ggctggcctg cctccgggcg
     2461 cgacgccccc tgccgcgcct ggagcgctgg ctcccgggac cgctccgaca gatcagcccc
     2521 tgaccggcga ggcggtcatc accatgcagc ctccgggcgc catcacgcca agccgcgcag
     2581 ctccgccagt cacaccgcct cctgcgacga cgccacgatg agcggcattt cgggcatgac
     2641 cggctttggc cgggccgacg gcgcgctgga cggctggagc tggagcgtcg aggcgcgctc
     2701 ggtcaacggg cgtaatctgg aggtccggtt ccggggtcca aacgggttcg acgatctcga
     2761 acggctgacc aaggcggcgg cgcaagcgcg gctgaaccgg gggcaggtca ccgtcggtgt
     2821 ccaggccaaa caagcgcagg gcagcgccgg cgcgctcaag gtcaatgagg ccgtgctggc
     2881 ccgctatctg gagctcgcca atcaattggc ggaggagggc gcgacgccgc cttcagccga
     2941 tggcttgctg ggcctgcgcg gggtgctcga cgtcgccgac gatgacatcg atgccgagac
     3001 acgtacgccg atcgaagccg ccatggccgt ctcagttggc gaggcgctgg acgcgttgaa
     3061 ggtgtcgcgc gaacgcgaag gcgcccagct gacgccggtc atcgccgact tcatcggcag
     3121 gatcgaggcc ctgatcggga aggccgaagg cgaggcgtcg gcgcagaccg atcatatccg
     3181 cgaacgattt ggccgccgca tggccgaact ggcgcccgac gcaacggggc tggaagaccg
     3241 tatcgtggtc gaggcggcgg cgctggcgac ccgggcggat gtgcgcgagg agctggatcg
     3301 gctgacggcg cacgtcgcct cggcccgcac tctgttgctt caaccgcccg caggacggaa
     3361 actcgacttc ctgatgcagg aattcatgcg tgaagcgaac accctgtgct cgaaatcggc
     3421 taccacgccg ctgaccgcca tcggactcga tctgaaggcg gtcatcgagc aacttcgaga
     3481 acaaatccaa aatgtcgaat gaccgtacgc cgcgccgcgg tgtcctgctg atcgtggcca
     3541 gcccctcggg cgccggcaag acttccctgt gccgtcgatt gatggccgat cacggcgggc
     3601 tggagctgtc cgtgtcgatg acgacgcgcg ggatccgtcc gggcgaggtc gcagatcgcg
     3661 actatcattt cgtcacgccg gaagcttttc aggcgttgat cgatcaggac gccttcctgg
     3721 agtgggccga tgttcacgga agccgctacg gcagtccgcg cgcgccaatc gacaaggcgc
     3781 tggaagaggg gcgcgatgtt cttttcgata tcgactggca gggcgcggaa cagg
//
LOCUS       DASVTM010000109         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_967935,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000109 DASVTM010000000
VERSION     DASVTM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967935"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1440)
                     /locus_tag="VF633_03160"
     CDS             complement(136..1440)
                     /locus_tag="VF633_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8470089.1"
                     /translation="MTDATTTREPITIKAPSPGKYRWVIMWLLFAAMVINYVDRQMIG
                     FLKPTLSVEMGWSETDYADIVFYFQATYALFYAIWGRLMDKIGARWGLGVAFAIWQVG
                     HILHAGARDLNHFIMARMVLGAGEAGGFPGGIKAVTEWFPKKERALATGLFNAGTNIG
                     AIVTPLIVPVLVLSFGWQMSFIITGLLGLIWLPIWLLVYRKPREQKRLSATELAYIEQ
                     DPADSDEKVGWLKLLTVKETWAYALGKFLIDPVWWMFLFWLPDFLGKRYGLDLKTFGP
                     PIVAIYLLSDVGSVGGGWLSSRMMHMGVSLNKARKLTMLLCAVLAVPVMFAAEASNLW
                     VAVLIIGLATAAHQGFSANLYALPGDVFPRKAVGSVVGIGGMLGGIGGMVFSKYVGQI
                     LEKIGTYTPIFVVAGSVYLIALLVIHLIMPKYAPAKVGGVTA"
     gene            complement(1534..2352)
                     /locus_tag="VF633_03165"
     CDS             complement(1534..2352)
                     /locus_tag="VF633_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEX8470090.1"
                     /translation="MTASSQTAQTEVQSPTATAEAFVSARLAARATPDYPGVIPATMA
                     DGYVIQEIAIGLYPDPIVGWKVGGVPPPKQEALGTHRLAGPIFARNRWPIDGDVSVAA
                     IEGGFTAIEAEFLARIGPNVDPAKTEWTAEEAADVVDAVFIGLEIAGSPLSAINDLGP
                     PIVASDFGNNAGIFVGPELNDWRAALDGVVVETVIDGVSVGTGGSPSLEGGAVESVRF
                     LLGHCAKRGRPLTQGMLVSTGAVTGVHRIYAGSEFTCDFRGLGVIKGKVVKAEA"
     gene            2519..>3388
                     /locus_tag="VF633_03170"
     CDS             2519..>3388
                     /locus_tag="VF633_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8470091.1"
                     /translation="MTDDQRAAKPGKRATINDVARVARVSKKTVSRVINLSPFVKGDT
                     RERVDAAIAELGFTPDPQARGLAFRRSFLVGLIYDNPSPTYVVNMQQGVLDALKGSGL
                     ELVVHPCNRNSETLIEDIRGFIERQRLFGVIMPPSMSEDERVIDLLRELDCPYVRIAS
                     ISLDEPQNMVVTNDHLGAAEAANRLADLGHTRIGLVRGPALFRSSAVRGGGFRDALAE
                     RGIPMQPEYEIEGAYTFESGVEAGHALLSLPEPPTAIFSLNDDMAIGVMQAARERGLE
                     LPRDLSMVGFDDLP"
BASE COUNT          634 a         1084 c         1079 g          591 t
ORIGIN      
        1 aacgatcatc atattataat acaaatggag cgccatccct tttgcactcc tctgcggccc
       61 gccgtcgggt gacgcgcaca aaaaagcggc ggtggatcgg gtccaccgcc gctctatagt
      121 ctcacgccgt gaggatcagg ccgtgacgcc gcccaccttg gccggagcgt atttcggcat
      181 gatcagatga atgaccagca gggcgatcag gtagaccgaa ccagcgacga cgaagatggg
      241 cgtgtaggtg ccgatcttct cgaggatctg gccgacgtat ttcgagaaca ccatgccgcc
      301 gatgccgccg agcatgccgc cgatgccaac gactgacccg acggcctttc ttgggaagac
      361 gtcgcccggc agggcgtaga ggttggcgga gaagccctgg tgcgccgcgg tcgccaggcc
      421 gatgatcagg accgcaaccc acaggttcga agcctcggcc gcgaacatga ccggcacggc
      481 cagcacggcg cacaacagca tggtcagttt gcgcgccttg ttcaggctga cgcccatatg
      541 catcatccgc gaggacagcc agccgccgcc caccgaaccc acatcggaca gcagatagat
      601 ggccacgatc ggcggaccga aggtcttgag gtcgaggccg taacgcttgc cgaggaagtc
      661 gggcaaccag aacaggaaca tccaccagac cgggtcgatc aggaatttgc ccagcgcata
      721 ggcccatgtt tccttgacgg tcagcagctt caaccagccc accttctcgt cagagtccgc
      781 aggatcctgc tcgatatagg ccagttcggt tgccgacaga cgtttttgct cgcgcggctt
      841 gcgatagacc agcagccaga tcggcagcca gataagaccc agcaggccgg tgatgatgaa
      901 ggacatctgc cagccgaagg acagtaccag aaccggaacg atcaggggcg tgacgatcgc
      961 cccgatattg gtgcctgcgt tgaacaggcc ggtcgccagt gcccgttcct tcttggggaa
     1021 ccactcggtg acggccttga tgccaccggg gaaaccaccg gcctcgccgg cgcccagaac
     1081 catgcgggcc atgatgaagt ggttcaggtc ccgggctccg gcatgcagga tgtggccgac
     1141 ctgccagatg gcgaaggcga cacccaggcc ccagcgggcc ccgatcttgt ccatcaaccg
     1201 gccccagatc gcgtagaaca gcgcgtaggt ggcctggaaa tagaagacga tgtcggcgta
     1261 gtcggtctcc gaccacccca tctcgacgga cagcgtcggc ttcaggaagc cgatcatctg
     1321 acggtcgacg tagttgatga ccatggccgc gaacagcagc cacatgatca cccagcggta
     1381 tttgccgggt gacggcgcct tgatcgttat cggctcacgt gtggtcgtgg cgtcggtcat
     1441 cgtgaaaaat cccgttcctg tactcgtatc ctcgcgcgtc ccgacctgtc tccgcatcat
     1501 gcggaccggc gggttggcgc ctcgtttcta gtcttaagcc tcggccttga cgaccttacc
     1561 cttgatcaca cccaggcctc tgaagtcgca ggtgaactca ctgcccgcat agatgcggtg
     1621 cacgccggtg acggcgccgg tcgagaccag catgccttgc gtcaggggcc ggccgcgttt
     1681 ggcgcaatgc cccagcagaa agcgcaccga ttccacggcc ccgccttcaa gcgatggcga
     1741 tccgccggtc ccgacggaga cgccgtcgat gacggtttcc acgacgacac cgtccagcgc
     1801 cgcgcgccag tcgttcagtt cggggccgac gaagatgccg gcgttgttgc cgaaatcgga
     1861 ggccacgatg ggcgggccga ggtcgttgat ggctgacagc gggctgcctg cgatttcaag
     1921 gccgatgaag acggcgtcga ccacgtcggc ggcttcttcg gcggtccatt cggtcttggc
     1981 tggatcgacg ttcggtccga tgcgggcgag gaattcagct tcaatggccg tgaagccgcc
     2041 ttcgatcgcc gcaaccgaga cgtcgccgtc gatcggccag cgattgcgcg cgaagatcgg
     2101 tccggcgagg cggtgagtcc cgagagcttc ctgtttgggc gggggaacgc caccgacctt
     2161 ccagccgacg atcgggtctg gatacagacc tatcgcgatc tcctggatca cgtagccgtc
     2221 ggccatagtg gcgggaatca cgcccggata gtcgggggtc gcacgcgctg cgagccgtgc
     2281 cgagacaaag gcctcggcgg tcgccgtcgg gctctggact tcggtttggg cggtctggct
     2341 ggaggccgtc ataggtctcc tcccttggta aacatcgtca cgaacacgaa agtccctcga
     2401 taagggccgg aatatgggcg ggcaaggaaa ccggtgtcaa tgcggttctt atgacacctg
     2461 tggcgcttct cgccgggcgc agctatgcct tggtcaaaag agaataagag gaacgcctgt
     2521 gacagacgac cagcgtgccg cgaagcccgg caagcgcgcg acgatcaacg acgtggcccg
     2581 ggtcgcccgg gtgtccaaga agacggtctc gcgggtcatc aacctgtccc ccttcgtcaa
     2641 gggcgacacc cgtgagcggg tcgacgcggc catcgccgaa ctcggcttca ccccggatcc
     2701 gcaggcgcgc ggcctcgcct tccgccggtc gttcctcgtc ggactgatct acgacaaccc
     2761 cagcccgacc tatgtcgtga acatgcagca gggcgtcctg gacgcgctta agggctcggg
     2821 gcttgagctc gtcgtccatc cctgcaatcg caactccgag accctgatcg aggatattcg
     2881 agggttcatc gagcgccagc gcctgttcgg cgtcatcatg ccgccgtcga tgtcggagga
     2941 tgagcgggtc atcgacctgc tgcgcgaact cgactgcccc tatgtccgca tcgcctcaat
     3001 ctcgctggac gagccacaga acatggtcgt gaccaacgac catctgggcg ccgccgaggc
     3061 cgccaaccgg ctggcggatc tgggccatac ccgaatcggt ctggtgcgtg ggcctgccct
     3121 gttccgttcc tcggcggtgc gtggcggcgg gttccgtgat gctctggcgg aacgcggcat
     3181 tcctatgcag ccggaatatg agatcgaagg cgcctacacc ttcgaatcgg gcgtcgaggc
     3241 cgggcacgcc ctgctctccc tgcccgaacc tccgacggcg atcttcagcc tgaacgacga
     3301 catggcgatc ggcgtgatgc aggcggcgcg cgagcgcggg ctggagctgc cgcgcgacct
     3361 gtcgatggtg ggtttcgatg acctgccg
//
LOCUS       DASVTM010000110         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_542263,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000110 DASVTM010000000
VERSION     DASVTM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542263"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /gene="lon"
                     /locus_tag="VF633_03175"
     CDS             <1..2111
                     /gene="lon"
                     /locus_tag="VF633_03175"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX8470092.1"
                     /translation="TRFTDREEYFEAEAVEIADDLGEPSQAEAMLRAVVEQFESYVKL
                     NKKVPPEALSSIPQITEPSKLADSVAAHLSVKIADKQGLLETIDVPKRLEKVYGLMEG
                     EISVLQVEKKIRSRVKRQMEKTQREYYLNEQMKAIQRELGETDDSRDEIMELEKRIRK
                     TKLSKEARTKAEAEVKKLRNMSPMSAESTVVRNYLDWLLAIPWGKTKQKAIDLDKAEA
                     ILEEDHYGLEKVKERIIEYLAVQARTNSLKGPILCLVGPPGVGKTSLAKSIAKATGRE
                     YVRMSLGGMRDEAEIRGHRRTYIGSMPGKIIQSMKKVKTTNAFILLDEIDKLGADWRG
                     DPSSALLEVLDPAQNNAFGDHYLEVDYDLSNIMFVTTANSLNMPQPLMDRMEIIRVSG
                     YTEDEKVEIAKRHVLPKVTTDNGLTPVEFVVPDETIRDLIRYYTREAGVRSLERALGG
                     LARKAVREMSKEGTTTITVDAEKLAKYAGVQKFRYGETDEEDQVGIVTGLAWTEFGGE
                     ILTIEAIKMPGRGRMTVTGNLKEVMKESVSAAASYVRARALAIGVKPPVFEKTDIHIH
                     VPDGATPKDGPSAGVAMTVAMVSVLTGVPIRKDIAMTGEITLRGRVTAIGGLKEKLLA
                     ALRSGIKTVLIPQENEKDLADVPDNVKSALEIVPISTADEALKWALVGSLTPVEWDEL
                     TEPLNPTPPPPVDGSVVVSH"
     gene            2266..2541
                     /locus_tag="VF633_03180"
     CDS             2266..2541
                     /locus_tag="VF633_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX8470093.1"
                     /translation="MTKAELIAAMAAGANISRDQAKLALEAFTDGVSESLKRGEDVRI
                     VGFGSFVAVDRKAGSARNPQTGETISRAASRTARFRIGEGLKSVLNG"
     gene            2580..2656
                     /locus_tag="VF633_03185"
     tRNA            2580..2656
                     /locus_tag="VF633_03185"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2614..2616,aa:Val,seq:tac)
     gene            2681..3055
                     /locus_tag="VF633_03190"
     CDS             2681..3055
                     /locus_tag="VF633_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470094.1"
                     /translation="MRLFSPAGATPVEFLRIRSNILHRAELVASPIAGVAEGSRAPYR
                     RLHIGTAITLLICGTSTYTRRDRASQFDAAFVFGLGCVERGRQRQTGCEQVEDGSHLQ
                     ARAEIAMGDEPDREREGREGGQ"
BASE COUNT          640 a          966 c         1011 g          512 t
ORIGIN      
        1 tgacacgctt caccgaccgc gaggagtatt tcgaggcgga agccgtcgag atcgccgacg
       61 acctgggcga gccttcacag gcggaggcca tgctgcgcgc cgtggtcgag cagttcgaaa
      121 gctatgtgaa gctgaacaag aaggtcccgc ccgaggccct cagctccatt ccccagatca
      181 ccgagccgtc gaagctggcc gacagcgtcg ccgcccactt gtcggtcaag atcgccgaca
      241 agcagggcct gctcgaaacc atcgacgtgc ccaagcggct ggagaaggtt tacggcctga
      301 tggagggcga aatctcggtc ctgcaggtcg agaagaagat ccgctcgcgc gtgaagcgcc
      361 agatggagaa gacccagcgc gaatattatc tcaacgagca gatgaaggcg atccagcgcg
      421 agctgggtga gaccgacgac tcgcgcgacg agatcatgga gctcgaaaag cgcatccgga
      481 agaccaagct cagcaaggaa gcgcgcacca aggccgaggc cgaggtcaag aagctgcgca
      541 acatgagccc gatgtcggct gagtcgaccg tggttcggaa ctacctcgac tggctgctgg
      601 cgatcccgtg gggcaagacc aagcagaagg cgatcgacct ggacaaggcc gaagccatcc
      661 tcgaggaaga ccactacggc ctcgaaaagg tgaaagagcg gatcatcgag tatctggcgg
      721 tgcaggcgcg caccaacagc ctcaagggtc cgatcctgtg cctcgtcgga cctcccggcg
      781 tcggcaagac ctcgctggcc aagtcgatcg ccaaggctac gggtcgtgaa tacgtccgta
      841 tgtcgctggg cggcatgcgc gacgaggccg agatccgtgg tcaccgccgc acctacatcg
      901 gctcgatgcc cggcaagatc atccagtcga tgaagaaggt gaagaccacc aacgcgttca
      961 ttctgctgga cgagatcgac aagctcggcg ccgactggcg tggggacccg tcctcggccc
     1021 tgctcgaggt gctggatccc gcacagaaca acgcgttcgg cgaccactac ctcgaggtcg
     1081 actacgacct gtccaacatc atgtttgtca ccacagccaa cagcctgaac atgccgcagc
     1141 ccctgatgga ccggatggag atcatccgcg tcagtggcta caccgaggac gagaaggtcg
     1201 agatcgccaa gcgtcacgtc ctgcccaagg tcacgaccga caacggcctg accccggtcg
     1261 agttcgtcgt gcctgacgag accatccgcg acctgatccg ctactacacc cgcgaagccg
     1321 gcgtgcggtc gcttgaacgg gctctcggcg gtctggcccg caaagctgtc cgcgaaatgt
     1381 ccaaggaagg cacgaccacc attacggtcg acgccgagaa gctggccaaa tacgcgggcg
     1441 ttcagaagtt ccgctacggc gagacggacg aagaggatca ggtcggcatc gtcactggtc
     1501 tggcctggac cgagttcggc ggcgagatcc tgaccatcga ggcgatcaag atgccgggcc
     1561 gcggtcgcat gaccgtcacc ggcaacctga aggaagtgat gaaggagtcc gtctccgccg
     1621 ccgcctccta cgtccgggcg cgtgcgctcg ccatcggcgt caagcctccg gtgttcgaga
     1681 agaccgacat ccacatccac gttcccgacg gcgcgacgcc caaggacggc ccctcggcag
     1741 gtgtggctat gaccgtggcc atggtctcag tcctgacagg cgtgccgatc cgcaaggata
     1801 tcgccatgac cggcgagatc accctgcgtg gtcgcgtcac ggccatcggc ggtctgaagg
     1861 agaagctgct cgcggccctg cgctccggca tcaagacggt gctgatcccg caggaaaacg
     1921 agaaggatct cgccgacgtg cccgacaacg tgaagtcggc gctggagatc gttccgatct
     1981 ccacggcgga cgaagccctc aaatgggctc tggtcggcag cctgaccccg gtcgaatggg
     2041 acgagctgac cgagccgctc aaccccacgc cgcctccgcc cgtggacggt tcggtcgtgg
     2101 tcagccacta acggtcgccg cttcaaggtt gatgtgtcgt taggtacgaa aagcacggtc
     2161 cgggcccccg ggccgtgttt ctcttttgac gggcacaaaa aagctggttt tactgtgggc
     2221 ttcacgacgg tgcgggggca tcacataggg ggcgaccgag catccatgac caaagccgag
     2281 ttgattgccg ccatggcggc aggcgccaat atttcccgtg accaggcgaa gttggcgttg
     2341 gaggctttca cggacggggt aagcgaatcg ttaaagcgcg gcgaggacgt ccgcatcgtg
     2401 gggttcggca gcttcgtggc cgtcgatcgc aaggccggga gcgcccgaaa cccccagact
     2461 ggagagacca ttagtcgcgc cgcttcccgc acggcgcgct tccgaatcgg cgaaggtctg
     2521 aaaagcgtct tgaacggtta gccggagaca ggcacaaggc acgtccaccg acgtggtgag
     2581 ggcggctagc tcagctggtc agagcatctc gtttacaccg agagggtcgg cggttcgaac
     2641 ccgtcgccgc ctaccacttt tctcgagaac gacagcgccg ttgcgtttgt tttcgccggc
     2701 gggggcgaca ccggtcgagt ttctgcggat tcgctcaaac atcctgcatc gggctgaact
     2761 ggtcgcgtcg ccgatcgctg gtgttgctga aggcagtcgg gctccctatc ggcggcttca
     2821 tattgggacg gcgatcacgc ttttgatctg cgggacatca acttataccc gccgtgaccg
     2881 ggccagtcag ttcgacgccg cctttgtttt cggccttggc tgtgtggagc ggggaaggca
     2941 acgccagacc ggctgcgagc aggtcgaaga cggttcgcac cttcaagctc gtgcggagat
     3001 cgcgatgggt gacgagccag accgggaaag agaggggcgg gagggcgggc aatagtcgcc
     3061 gcactgaggt gtcggattcg ccaagccgaa gggtcatgat ccccacgccg gctcccgctt
     3121 tcaccatgg
//
LOCUS       DASVTM010000111        12066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_224388,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000111 DASVTM010000000
VERSION     DASVTM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224388"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2188
                     /gene="uvrB"
                     /locus_tag="VF633_03195"
     CDS             <1..2188
                     /gene="uvrB"
                     /locus_tag="VF633_03195"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX8470095.1"
                     /translation="KGRPEFIDKTWAPHRPSRPQKSEGGVPLVIRSEFEPAGDQPAAI
                     AELVEGIGEKEHDQVLLGVTGSGKTFTMAKVIEQTQRPALILAPNKTLAAQLYSEFKS
                     FFPDNAVEYFVSYYDYYQPEAYVPRTDTYIEKDSSQNEQIDRMRHSATRSILERDDVI
                     VVASVSCIYGIGSVETYTAMTFTLKIGDQIDEAKLRADLIALQYKRNDVTFERGMFRK
                     RGDTIEIFPVHQEDRAWRVSLFGDEIESIQEFDPLTGKKTAELNEITVYAASHYVTPR
                     PTLNQATIGIKAELKETLDWMIENGKLLEAQRLEQRTRFDLEMMEATGSCAGIENYSR
                     WLTGRSPGEPPPTFFEYIPDNALLFVDESHVTVGQINGMFRGDYRRKSTLAEYGFRLP
                     SCIDNRPLKFDEWDAMRPQTMFVSATPGPWEMERTGGVFVEQVIRPTGLIDPPVEIRP
                     VSGQTRNQVDDVIDEVKAVARAGYRSLVTVLTKKMAEDLTEYMHEQGVRVRYMHSDVD
                     TLERIEILRDLRLGTFDVLIGINLLREGLDIPECGLVAILDADKEGFLRSETSLIQTI
                     GRAARNVDGRVILYADRMTGSMERAIAETDRRRERQVAYNLEHGITPESVKRDIKEIL
                     NSPYEKDRVLVPIGVKEESRPFTGTNFQAALKDLEGRMREAASNLEFEEAGRLRDEIK
                     KMKLLDLEFANDLMTAEGQDVDKSAPKRWRADAAAEKQEAFRKGRL"
     gene            2278..2829
                     /locus_tag="VF633_03200"
     CDS             2278..2829
                     /locus_tag="VF633_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470096.1"
                     /translation="MVSAGLPLFGYLRYTRLENHAAAGREMSLACFFGFLPVLTAILI
                     SYLVKGTAGVGPAIQFQVDTGSIFLVCAALLSSYIYVLWGEQLDPGLAPFPHKTAFSL
                     IFVGMIVAWAIVMGLQALNAKNVGATISLDSDALRALTIVSLVMSFTCLYLMFVYRSA
                     IKPGYAGITREETESFSSDWQGR"
     gene            2826..4031
                     /locus_tag="VF633_03205"
     CDS             2826..4031
                     /locus_tag="VF633_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEX8470097.1"
                     /translation="MSSIILPETETQGQSPDLLPPDPDKQVLLIPALKVSQPIGESFI
                     ASIPFKTLIRMSRFDVRRIMQVERPFETYLGIQRPVDPKRREQLQAYVNFKDANFPGA
                     VIIAIDTDYAKFDEEHKTLEVRNWRDGEENPSVNLGDVARVIDGQHRIAGLENYRGEQ
                     FDIPVTIFVGADLSDQAYIFSTVNLEQTKVNKSLSYDLFALAKTRSPQKTAHNIAVAL
                     DRDPNSPFYQKIKRLGFAQRTGGTETLTQATFVEMLMSTISTNPKVDRDLLLKGKALD
                     RVRSDRPGQLVFRNLFIDEKDLVIAENVNNYFLAVSERWSTAWWAAGTGIILGRTNGF
                     RALMRFLPMAYRYSLTSGEVVSKNAFALLFEKIRVDDIYFNSERFSPGSGGESSLLQF
                     IKDQLPFVD"
     gene            complement(4045..4692)
                     /locus_tag="VF633_03210"
     CDS             complement(4045..4692)
                     /locus_tag="VF633_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HEX8470098.1"
                     /translation="MAYDSEQPDPNLNLRAFANGERFRTILADPPWQFQNRTGKVAPE
                     HKRLNRYSTMDLKAICALPVAEVAQETAHLYMWVPNALLPEGLEVMKAWGFNYKSNLI
                     WHKIRKDGGSDGRGVGFYFRNVTEILLFGTRGKNARTEAPGRRQVNMLQTRKREHSRK
                     PDEQYELVEACSTGPFLELFARGERPGWAVWGNQADETYAPTWKTYSYNSSVAAE"
     gene            complement(4698..5351)
                     /locus_tag="VF633_03215"
     CDS             complement(4698..5351)
                     /locus_tag="VF633_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HEX8470099.1"
                     /translation="MSNLRFVPEDLQALYEVHEWRNGLAVLAAARPDEWEDLKAALSE
                     FRLYATEIIKKGGNRSAIAIRLDSLLEQRNWVEHRFETSIRVDTDERQSPTHKVDMYK
                     NRVAFETEWNNKDPFYDRDLNNFRLLFDLRAIDVGVIVTRCDELQQLVVELGRDKGSY
                     GNSTTHMSKLLPRLEGGGGGGCPVVVFGIRREAFVDDRQNPVAPAKATTVTSGDDEE"
     gene            6003..6701
                     /locus_tag="VF633_03220"
     CDS             6003..6701
                     /locus_tag="VF633_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470100.1"
                     /translation="MQPHPLTTYVTASPATWSLIRGAYLSGLSAPTVAVRFGVSVSAI
                     RKRARREGWTKAVFAATATPWSGWRTGPAPDSSPSENGGRSSHGLGVGSADEEAILEG
                     WRSPIHIRPGDLARRALANAAHAVKTGAGLNAVRLARAAAEIARLDGLLDWAVDDPAV
                     TDEQAEGRDNVMRLFLREQALKLARILADGGELPAPYAEARAELERLRAMSEADPDAP
                     CPDIGSADPAGCAS"
     gene            complement(6770..7444)
                     /locus_tag="VF633_03225"
     CDS             complement(6770..7444)
                     /locus_tag="VF633_03225"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX8470101.1"
                     /translation="MSKVLGVLGGMGPAATVAFLARVQALTPAEGDADHIRVFMDMNP
                     QVPDRNKTPELAAVVLGEMAARLRDAGAQVLAMPCNTAHAQASGIHAVAEAAGIPFID
                     MIAATTATVVSTGQTRVGILATPGGEALYVTALTAAGRAPLLLEGPDREAFMARVYGV
                     KAGDTGDAARARMKALAAALVARGAEVLVAGCTEVPLLLSAADVTVPLVDSAEVLAEA
                     CVFACL"
     gene            complement(7445..8443)
                     /locus_tag="VF633_03230"
     CDS             complement(7445..8443)
                     /locus_tag="VF633_03230"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HEX8470102.1"
                     /translation="MHLARFPRVRLAHLPTPLEPLPRLSEALGVELWIKRDDCTGLAG
                     GGNKTRKLEFLLGDAFEQGADTLVTQGAVQSNHVRQTAAAAASHGLKCEIILEERTGS
                     KAIDYTRNGNVLLDRLFDAQVRTVPGGTDMVAELEITAEAVRKRGGKPYIIPGGGSNP
                     IGALGYADCAREIVVQADELDMAIDRIVTATGSAGTHAGLVAGLAVMGADIPVLGIGV
                     RAPKEKQEANVRKLARETAALLGRPDAVTDAMVVADCDYVGEGYGLVDDAVIEALKLA
                     ARTDGIVLDPVYTGKAMKGLIALARAGRFENETVVFLHTGGAQGLFGYQGEIEGSL"
     gene            8570..9037
                     /locus_tag="VF633_03235"
     CDS             8570..9037
                     /locus_tag="VF633_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470103.1"
                     /translation="MIATEDRNGLFNGLPARGSTAYVELLVFLNANRSAVLAALPWVA
                     AEIAGNEGFEVMLDFVCRLGGRRLYISARLQEFSRATDSAFGLATHRNLLRGAGVSSL
                     LEAPSAWGVFLVFRRVALARALAEGVSKKAAARLFGVTERSLRGPQRTESAHD"
     gene            9110..9847
                     /locus_tag="VF633_03240"
     CDS             9110..9847
                     /locus_tag="VF633_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470104.1"
                     /translation="MPSTDDLWSMYGHQILKFAAPDFDPTKQQFSLASSTLSVDLGNA
                     DPAIVNGYIYNLGNTIPGPSPSYMPGSALFTSYQRFLDAIDLHGDTNPNDDAGINIAA
                     ANMNAAQTNFLSTQGAAITAYTTYKIVAPNVTFSDYVTQQYPTYIQAKNALDATTSAW
                     QQLMTQKYGQGYEIIANARNRLSPTGGASDILSANSYNMAVKTGAAAPAGSVPVLPGG
                     TPQPPANALVPSFDPSFALQSFGSVYQ"
     gene            9896..10459
                     /locus_tag="VF633_03245"
     CDS             9896..10459
                     /locus_tag="VF633_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470105.1"
                     /translation="MDGSAASSSFSDMGWSSESAGMFSYGFFDVWGETSSSSDTQTSF
                     AMDSSFHMQIDFTGMNTFTISPGGWFDLGIVNSFRNQLLPNSPDLFSSAGALARIPYQ
                     AVIGFQPSITLSMSQENFSSFQSTFQSQTTAGMGFGPFVVGETHSSSYANKSGAAFDA
                     QSATVTVSPPPSTVPVLLGIISTRLDV"
     gene            10522..10905
                     /locus_tag="VF633_03250"
     CDS             10522..10905
                     /locus_tag="VF633_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470106.1"
                     /translation="MPIQSVQGAAIPNGVVFNANQRDDATTAIGLVQLADVWRGGNTI
                     ANTQRVRVDSQGNQVNGRANLQAQINGISGASTIACVLVDASVGMAGGQAVAAQQLYA
                     IRQIRGALQNSLNTGMVYTLTGNPT"
     regulatory      10961..11069
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11211..>12066
                     /locus_tag="VF633_03255"
     CDS             11211..>12066
                     /locus_tag="VF633_03255"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX8470107.1"
                     /translation="MRETGMIPTGERAGSRKVYMAGELFPDIRVPFREVAVHPSANEP
                     PVTIYDSSGPYTDPTAVIDIKRGLPLVKSSWQLDRGDIAPVLDPREVKPEDNGHASGK
                     NLAPRFDTSRHKVFKGVEGRPVTQYEYANAGIITPEMEYVAIRENLRREQTAPCIRDG
                     EDFGASIPDFVTPEFVRSEIARGRAIIPHNINHPEVEPMIIGRNFLVKINANIGNSAV
                     LSSVDDEVDKLVWATRWGADNVMDLSTGRNIHNIRDWIIRNSSVPIGTVPIYQALEKV
                     NGVAEDLTW"
BASE COUNT         2283 a         3983 c         3656 g         2144 t
ORIGIN      
        1 gaagggccgg cccgagttca tcgacaagac ctgggcgccg catcgcccgt cccggccgca
       61 gaaatccgaa ggcggcgtgc cgcttgtcat cagatcggag ttcgagcccg ccggcgacca
      121 gcccgcggcc atcgccgagc tcgtcgaggg catcggcgag aaggaacacg accaggtcct
      181 gctcggcgtc accggctccg gcaagacctt caccatggcc aaggtcatcg aacagaccca
      241 gcgccccgcc ctgatcctcg cccccaacaa gaccctcgcc gcccagctct attccgagtt
      301 caagagcttc ttcccggaca acgccgtcga gtatttcgtc tcctactacg actactacca
      361 accggaagct tacgtaccca gaacagacac ttacatcgaa aaggactcgt cgcagaacga
      421 gcagatcgat cgcatgcggc actcggcgac gcggtcgatc ctcgagcggg acgacgtcat
      481 cgtcgtggcg tcggtcagct gcatctacgg tatcgggtcg gtcgagacct acaccgccat
      541 gaccttcacg ctgaagatcg gggaccagat cgatgaggcc aagctgcggg ccgacctgat
      601 cgcgctgcag tacaagcgta acgacgtcac gttcgagcgc ggcatgttcc gcaaacgtgg
      661 cgacaccatc gagatattcc cggtccacca ggaggatcgc gcctggcggg tgtccctgtt
      721 cggcgacgag atcgagtcga tccaggagtt cgaccccctc accggcaaga aaaccgccga
      781 gctcaacgag atcaccgtct acgccgcctc ccactacgtc accccgcgcc cgacgctcaa
      841 ccaggccacc atcggcatca aggccgagct caaggagacg ctcgactgga tgatcgagaa
      901 cggcaagctg ctggaggccc agcgcctcga acagcgcacc cgtttcgatc tggagatgat
      961 ggaggccacc ggctcctgcg ccggcatcga gaactattcc cgctggctga cgggccgcag
     1021 cccgggcgag ccgccgccga ccttcttcga atacatcccc gacaacgccc tgctgttcgt
     1081 cgacgagagc cacgtcaccg tgggccagat caacggcatg ttccggggcg actaccgccg
     1141 caagtcgacg ctggccgaat acggtttccg cctgccctcc tgcatcgaca accgcccgct
     1201 caagttcgac gaatgggacg ccatgcggcc ccagacgatg ttcgtcagcg ccacccccgg
     1261 cccgtgggag atggagcgca ccggcggcgt cttcgtcgaa caggtcatcc gccccaccgg
     1321 cctgatcgac ccgccggtgg agatccgccc ggtctcgggc cagacccgca accaggtcga
     1381 cgacgtcatc gacgaggtca aggccgtggc ccgcgccggc taccgctccc tggtcaccgt
     1441 cctgaccaag aagatggccg aggacctgac cgaatacatg cacgaacagg gcgtccgcgt
     1501 ccgctacatg cactccgacg tcgacacgct ggagcggatc gaaatcctgc gcgacctgcg
     1561 tctgggcacg ttcgacgtcc tgatcggcat caacctgctg cgtgagggcc tcgacatccc
     1621 cgagtgcggt ctggtcgcca tcctcgatgc ggacaaggag ggtttcctgc gctccgagac
     1681 ctccctgatc cagaccatcg gccgcgccgc gcgcaacgtc gacggccgcg tcatcctcta
     1741 cgccgaccgc atgacgggct cgatggagcg cgccatcgcc gagaccgacc gccgccgcga
     1801 acgccaggtc gcctacaacc tcgaacacgg catcacgccc gagagcgtca agcgcgacat
     1861 caaggaaatc ctcaactccc cctacgagaa ggaccgggtc ctggtcccca tcggcgtcaa
     1921 ggaggagtca cgccccttca ccggcacgaa cttccaggcc gccctcaagg acctcgaagg
     1981 ccgcatgcgc gaagccgcct ccaacctcga gttcgaggag gccgggcggc tcagggacga
     2041 gatcaagaag atgaagctgc tggatctgga gttcgccaac gacctgatga cggcggaagg
     2101 tcaggacgtc gacaagtcgg cgccgaagcg ctggcgggct gacgcggcgg cggagaagca
     2161 ggaggcgttc aggaagggaa ggctgtaggc acgggttagc tagcgataaa tcgctggcat
     2221 ttgcttgcga aagttgcaga agcgtgttcc ctcgtgcgct aaagctgggg gaacaatatg
     2281 gtatcggcag gattgccgct gtttgggtat cttcgttaca cgcggctaga aaaccatgct
     2341 gcggccgggc gcgagatgtc acttgcgtgc ttcttcggtt ttttgcccgt cttaaccgcg
     2401 attttgatta gctacctcgt gaagggtacc gcaggcgttg gacccgccat acaatttcag
     2461 gtcgacactg gctcaatctt cctggtctgc gcggcgctac tgtcatcata catatacgtt
     2521 ctgtggggcg agcaactcga tccgggattg gccccatttc ctcacaagac tgcgttttcg
     2581 ctaattttcg tcgggatgat tgtcgcgtgg gcaatcgtca tgggcttgca agctttaaac
     2641 gccaagaatg tgggtgcaac gatcagctta gacagtgacg cccttcgagc tttaacaatc
     2701 gtctcgctcg ttatgtcttt tacgtgcctg tacttaatgt tcgtctacag aagcgctata
     2761 aagcccggtt acgccgggat cacccgcgaa gaaacagaat cattctctag tgattggcaa
     2821 ggtcgatgag cagcattatt ctgcctgaga cagaaaccca aggtcagtcc cctgatctgt
     2881 tgccgccgga tccagacaaa caagttttgc taatacccgc gctaaaggtt tcccaaccta
     2941 tcggcgagag tttcattgcc tcgataccat tcaagacgtt gatcagaatg tcccggttcg
     3001 atgttagacg catcatgcag gttgagcgcc cgttcgagac atatttgggc attcaacggc
     3061 ctgtggatcc aaaacggcgc gaacagcttc aagcttacgt aaatttcaag gatgccaatt
     3121 tccccggcgc agtcataatt gcgatcgaca ccgactacgc caagtttgat gaagaacata
     3181 agacgcttga ggtcaggaac tggcgtgatg gagaggaaaa tccatccgtt aatctaggcg
     3241 acgtcgcaag agttattgac ggacaacatc gtattgctgg cctcgaaaat tacagaggcg
     3301 aacagtttga tataccggta actatatttg tgggtgccga cttgtcagac caagcttata
     3361 tattttctac ggttaacctc gagcagacca aagtaaacaa gagtctctcc tacgacctat
     3421 ttgcgctagc gaagacacgc agcccgcaaa aaactgctca caatattgct gttgcactcg
     3481 atagggatcc gaacagcccg ttctatcaga aaatcaagcg cttggggttt gcgcagcgta
     3541 ctggcgggac ggaaaccctg acccaagcga cgtttgtgga aatgctgatg tccacgatct
     3601 cgacaaaccc caaggttgat cgtgatttgc tcctcaaagg caaagctctt gaccgtgtga
     3661 gaagtgatcg cccgggacag ctggtatttc gaaatctctt catcgacgag aaggatctcg
     3721 tcatcgccga aaatgtgaac aactatttct tggcagtatc ggaacgctgg agcaccgcat
     3781 ggtgggctgc gggtacggga attattcttg ggcgcaccaa tgggtttaga gcgctcatga
     3841 gattcctccc gatggcctat cgttatagcc tgacgtcggg cgaagttgtt tcaaagaatg
     3901 cgtttgcttt gctcttcgag aaaatccgcg tcgacgatat ctattttaac tcagaacgtt
     3961 tttcgcccgg gtctggggga gaatcctcgc ttctccaatt tatcaaagac cagctcccat
     4021 tcgtagactg atgttgctta gaatctactc tgcggcaacg gacgagttgt aagaatacgt
     4081 cttccacgtt ggcgcataag tctcatcggc ctgattgccc catacggccc agcccggtcg
     4141 ctcgccgcgt gcgaacagct ccagaaatgg ccccgtgctg cacgcctcga ccagttcgta
     4201 ctgttcgtca ggcttgcggc tgtgctcacg tttgcgagtc tggagcatgt tgacctgacg
     4261 ccgtccggga gcctcggtac gcgcattctt gccgcgcgtt ccgaacaaca gaatctcggt
     4321 tacgttgcga aaatagaacc cgacgccccg gccatctgaa ccgccgtcct tgcggatctt
     4381 gtgccagatc aagttggact tgtagttgaa gccccaagct ttcatgacct ccaatccttc
     4441 aggaagaagc gcgttcggca cccacatata gaggtgcgcc gtttcctgag cgacctcggc
     4501 aacaggcaat gcgcagatcg ctttaaggtc catcgtcgag tagcgattca ggcgcttgtg
     4561 ttccggtgcc accttgccgg tgcgattttg aaactgccaa ggcggatcgg ccaagatggt
     4621 gcggaaccgc tcgccgttgg caaatgcacg caagtttagg ttcgggtcgg gctgctctga
     4681 atcataagcc atcgctgtca ttcctcatca tcgcctgaag tcacggtcgt agccttagcg
     4741 ggcgccaccg ggttttgcct gtcgtccaca aaagcttcgc ggcgaatgcc gaaaacgacg
     4801 actggacagc caccgccgcc gccgccttcg aggcgcggca aaagcttgct catgtgggtc
     4861 gtagaattgc cgtagctgcc cttgtcccgt cccaattcga cgaccagctg ttggagctcg
     4921 tcgcaacgcg tcacgatcac tccaacgtcg atcgcccgca agtcgaagag aaggcggaag
     4981 ttgtttaggt cgcggtcata aaatggatcc ttgttgttcc actcagtctc gaaggcgacc
     5041 cggttcttgt acatatccac tttatgggtc ggtgattggc gctcgtccgt gtccacgcga
     5101 atagatgtct cgaaccggtg ttcgacccag tttcgctgtt ccaggaggct gtctaaccgt
     5161 atggcgatgg ccgatcggtt tccgcccttt ttgataatct ccgtggcgta taaccggaac
     5221 tctgacagcg cagccttgag atcttcccac tcgtccggtc gcgctgctgc tagcaccgcc
     5281 aagccattcc tccactcgtg aacttcgtag agcgcttgca ggtcttcggg cacgaatcgg
     5341 aggttggaca tgcatcaggg taacctagtg gcggcctaga tctaggacac tcgaagttca
     5401 ccatatgttc tggcagaagt cgagtgcatc aaaagatggt ccaccttcaa cgtttttcgc
     5461 caagatccgg caacggcttc gagtgttgtg ggactgcgac tagctggtgt ttcagcgggc
     5521 gcgggacgca tcggcggacc tggcaggttc gcgctctcgc gcgccgctgg gtctcccggt
     5581 ctcgctacgc tcgccggagg atgacggaag aagatgggag gcgtggtgcg ttctcctccc
     5641 catcggagat ggggaggtgg caggacgccg cttgcgtcgt gacggagggg gcgaccgggt
     5701 ctcgccggtg acctctttcg cacactggcc ttgtccagcg agcgttccac ccagcaccgt
     5761 gtcgccctct cctgacgccc ttcggtcgtc tgtcctctcc atcgccctcc ggcgatggag
     5821 aggtgatgcc catacccgcg cccgaattcc ccgaccccgc acgaacgccc ctcgggtgga
     5881 gacactcgac cctaatagtt tgaacccttt gcgaccctgc ggtggagaca gtggccccat
     5941 tcggcgcggc atagtcggcg gcggggctag agtctggcgt ctcaggaccg tcgagatcat
     6001 ccatgcagcc tcatccgctc accacctatg tcaccgccag ccccgcgacc tggagcctga
     6061 tccggggcgc ctatctgtcg gggctgtcgg cgccgaccgt ggcggtgcgg ttcggggttt
     6121 cagtcagcgc gatccgcaag cgcgcccggc gcgagggctg gaccaaggcg gtgttcgccg
     6181 ccacggccac gccctggtcg ggctggcgaa ccggcccggc acctgacagc tcgccctccg
     6241 aaaacggagg ccgttcgtct cacggcctcg gcgtcggctc ggccgatgag gaggccatcc
     6301 tcgagggctg gcgctcgccg atccatatcc ggccgggcga tctggcccgc cgcgccctcg
     6361 ccaacgccgc ccatgcggtc aagaccgggg ccggcctcaa cgccgtccgc ctcgcgcgcg
     6421 ccgctgccga gatcgcgcgg ctggacggtc tgctggactg ggccgtggac gaccccgccg
     6481 tcaccgacga acaggccgag ggccgcgata acgtcatgcg gctgttcctg cgcgaacagg
     6541 cgttgaagct ggcgcgcatt ctggcggacg gcggcgaact gcccgctccc tatgccgagg
     6601 cccgcgccga gctggaacgc ctgcgggcga tgagcgaagc cgaccccgac gccccttgcc
     6661 ccgatattgg gagcgccgat cccgccgggt gcgcatcatg agccgggtcg actgtctcca
     6721 cattccacct ttcgagcatt ttccggggac cggagacact cgacccaatt cagaggcagg
     6781 cgaacacgca ggcctcggcg agaacctcgg cggagtcgac cagaggcacg gtgacgtcgg
     6841 cggcggacag cagcagcggc acctcggtgc agccggcgac cagaacctcg gccccgcgcg
     6901 cgaccagagc ggcggccagc gccttcatcc gggcgcgggc ggcgtcgccc gtgtcgcccg
     6961 ccttgacgcc atagacccgg gccatgaagg cctcacggtc ggggccttcg agcagcagag
     7021 gcgcgcgccc cgccgccgtc agggccgtga cgtacagcgc ctcaccgccc ggggtggcga
     7081 ggatgccgac gcgggtttgt cctgtggaga cgaccgtcgc cgtggtcgcg gcgatcatgt
     7141 cgatgaaggg gatgcccgca gcctcggcga ccgcatggat gcccgacgcc tgggcgtggg
     7201 cggtgttgca gggcatggcc aggacctggg ccccggcgtc ccgcagccgc gccgccatct
     7261 cgcccagcac gaccgccgcc agctcgggcg tcttgttgcg gtcggggacc tgggggttca
     7321 tgtccatgaa gacgcggatg tggtcggcgt cgccctcggc cggggtcagg gcctgcaccc
     7381 gcgccagaaa cgccaccgtc gcggcggggc ccatgccgcc gagaacgccc aaaaccttcg
     7441 acatctagag cgagccctcg atctcgccct gatagccgaa caggccctgc gccccgccgg
     7501 tgtggaggaa gaccacggtc tcgttctcga accgacccgc gcgggccagc gcgatcagcc
     7561 ccttcatcgc cttgccggta tagaccgggt ccagcacgat gccgtcggtg cgggcggcga
     7621 gcttcagcgc ctcgatcacc gcgtcgtcga ccagcccgta gccctcgccg acatagtcgc
     7681 agtcggccac gaccatggcg tcggtgacgg cgtcgggacg gcccagcagg gcggcggtct
     7741 cacgcgccag cttgcggaca ttggcctcct gtttctcctt cggcgcgcgc acgccgatgc
     7801 cgaggaccgg gatgtcggcg cccatgacgg ccagaccggc gacgaggccc gcgtgggtcc
     7861 cggcgctgcc cgtggcggtg acgatgcggt cgatggccat gtcgagctca tcggcctgga
     7921 ccacgatctc gcgggcgcag tcggcatagc ccagcgcgcc gatcgggttg gagccgccgc
     7981 cggggattat gtagggcttg ccgccgcgct tgcgcaccgc ctcggccgtg atctcgagct
     8041 cggcgaccat gtccgtgccg ccggggacgg tgcggacctg cgcatcgaac agccggtcca
     8101 gaagcacgtt gccgttgcgg gtgtagtcga tcgccttgga gcccgtgcgc tcctcgagga
     8161 tgatctcgca tttgagcccg tgcgaggccg ccgccgccgc cgtctggcgc acatggttgg
     8221 actggaccgc gccctgggtg accagagtgt cggcgccctg ttcgaaggca tcgccgagca
     8281 ggaactcgag cttgcgcgtc ttgttgccgc cgcccgcgag gccggtgcag tcatcccgct
     8341 tgatccacag ctcgaccccc agcgcctcgg acagccgcgg cagaggctcc agcggcgtgg
     8401 gcagatgggc gagacgaacg cgagggaagc gggcgaggtg catgggcggg ggctccggtt
     8461 gtggaggccg ttctacgcgg ttcggaggag cggcgaaaca gcggacaggg gcaagaatat
     8521 tcgtgtaacg gttgcatgcg gatgtcgcag tcggagctcg agcatggata tgatcgccac
     8581 cgaagaccgg aacggcctgt tcaacggcct gcccgccagg ggatcgacgg cgtacgtcga
     8641 actgctcgta ttcctcaacg ccaacaggtc agccgtgctc gcggcgctgc cctgggtcgc
     8701 ggcggagatc gccggcaacg aaggcttcga ggtgatgctc gatttcgtct gccggctggg
     8761 cgggcgccgg ctttacatat cggcacgatt acaggagttc tcgcgcgcga cagacagcgc
     8821 attcgggctg gcgacccacc gcaacctgct gcgcggggca ggcgtctcgt cattgctgga
     8881 ggctccgtcg gcgtggggcg tcttcctcgt cttccgccgc gtcgcactgg cccgggccct
     8941 cgcggaaggc gtgtcgaaga aagccgcagc ccggctgttc ggcgtcaccg aacgctccct
     9001 gcgcggcccc caacgaacgg agtcggcgca cgactgagga acccttccgc atacacgcct
     9061 aacgtgcacg cgcgcatggt gctggtctcg caacgagaaa ggacaagaca tgcccagcac
     9121 ggacgacctc tggtcgatgt acggccacca gatcctcaaa ttcgccgccc ccgacttcga
     9181 cccgaccaag cagcagttct cgctggcctc gtcgacgctg tcggtcgacc tgggcaacgc
     9241 cgacccggcc atcgtcaacg gctatatcta caacctcggc aacacgatcc ccggaccgag
     9301 cccgtcctac atgcccggca gcgcgctgtt caccagctac cagcgcttcc tcgacgccat
     9361 cgacctgcac ggcgacacca acccgaacga cgacgccggg atcaacatcg cggcggccaa
     9421 tatgaacgcc gcccagacca atttcctcag cacacagggc gccgcgatca cggcctatac
     9481 gacctacaag atcgtggctc cgaacgtgac cttctccgac tacgtcaccc agcagtaccc
     9541 gacctacatc caggccaaga acgcgctcga cgccacgaca tcggcctggc agcagctgat
     9601 gacgcagaaa tacggccagg gctacgagat catcgccaac gcccgcaacc ggctcagccc
     9661 taccggcggc gcgtccgaca tcctgagcgc caacagctac aatatggcgg tcaagaccgg
     9721 ggcggcggcg cccgccggat cggtgccggt cctgccgggt ggaacgcccc agccgcccgc
     9781 caacgcgctg gtccccagct tcgacccctc gttcgcactg cagagcttcg gctcggtcta
     9841 ccagtaatgg caggcgaaca gtgcgtcgaa ggccaagcct gccgtctcga tcgtcatgga
     9901 cggcagcgcg gcctcgtcca gcttttcgga catgggctgg agcagcgaat cggcggggat
     9961 gttctcgtac ggcttcttcg acgtctgggg cgagacctcg tcgtcatcgg acacccagac
    10021 cagctttgcg atggattcca gcttccatat gcagatcgac ttcacgggga tgaacacctt
    10081 caccatctcg cccggcggct ggttcgacct cggcatcgtc aacagcttcc gcaaccagct
    10141 gctgcccaac tcgccggacc tgttttcgtc cgccggggcc ctggcccgca tcccgtatca
    10201 ggcggtgatc ggcttccagc cgtccatcac cctgtcgatg tcgcaggaga atttctcgtc
    10261 gttccagtcg accttccagt cgcagacgac cgccggcatg ggctttggtc cgttcgtcgt
    10321 cggcgagact cactcctcga gttacgccaa caagtcggga gcggccttcg acgcgcagag
    10381 cgccaccgtc accgtgtcgc cgccacccag caccgtgccg gtcctgctgg gcatcatcag
    10441 cacccgcctc gacgtctgac catcaacagc cggcggcgtc gtcgccggcc actttttcat
    10501 catcctcgat cggagagtcc gatgcctatt caatccgtgc agggcgccgc cattccaaac
    10561 ggcgtggtct tcaacgcaaa ccagagagac gacgccacga ccgctatcgg tctggtccaa
    10621 ctcgctgacg tctggcgagg cggcaacacg atcgccaaca cccagcgtgt tcgggtggac
    10681 agccagggca atcaggttaa cgggcgcgcc aaccttcagg cccagatcaa cggcatcagc
    10741 ggtgcgagca cgatcgcttg cgtgctggtc gacgccagtg tgggtatggc gggcggtcaa
    10801 gccgtcgcgg cccagcagct ctatgccatc cgccagatcc gtggcgcgct gcagaacagc
    10861 ctcaacaccg gcatggtcta caccttgacc ggcaatccta cctgaacggc gatcctgaat
    10921 ctgacccggt tgagccccgc gctcatccgg gttaggctgc ctgcgttctc cggggggtcg
    10981 cgatcgagcg gctgagattg gcgtagccgc ctgacccgtc gaacctgatc cgggtcatgc
    11041 cggcgaaggg atgggaaacg ctttggtcga cgcccccaat ttgggcaaca ggaccgttcc
    11101 gcccggagca ggcagccctc aaaccgtttg aggccgccat gaacatcgcc gtcaccattc
    11161 ccgaagaacc caacgtcgaa ctggcgttga aagacgcgcg cgatgcggtg atgcgcgaga
    11221 cggggatgat ccccaccggc gaacgggccg ggtcgaggaa ggtctatatg gcgggcgagc
    11281 tgttccccga catccgcgtg ccgttccgcg aggtcgccgt ccatccgagc gccaacgagc
    11341 cgccggtgac gatctatgac agctccggcc cctataccga cccgacagcg gtcatcgaca
    11401 tcaagcgcgg cctgccgctg gtcaaatcca gctggcagct ggatcgcggc gacatcgccc
    11461 ccgtgctgga cccgcgcgag gtcaagcccg aggacaacgg ccatgcctcg ggcaagaacc
    11521 tcgccccgcg cttcgacacc tcccgccaca aggtgttcaa gggcgtcgaa ggccgccccg
    11581 tcacccagta cgaatacgcc aacgccggga tcatcacgcc cgagatggaa tacgtcgcca
    11641 tccgcgagaa cctgcgccgc gagcagaccg cgccatgcat ccgcgacggc gaggatttcg
    11701 gcgccagcat ccccgacttc gtcacgcccg agttcgtccg cagcgagatc gcgcgcggcc
    11761 gggccatcat cccgcacaac atcaaccacc ccgaagtcga gccgatgatc atcgggcgga
    11821 acttcctggt gaagatcaac gccaacatcg gcaactcggc ggtcctcagc agcgtcgatg
    11881 acgaggtcga caagctggtc tgggccaccc gctggggcgc cgacaacgtc atggacctgt
    11941 cgacgggccg caacatccac aacatccgcg actggatcat caggaactcg tccgtcccga
    12001 tcggcacggt gccgatctat caggcgctgg aaaaggtcaa cggcgtggcc gaggacctga
    12061 cgtggg
//
LOCUS       DASVTM010000112         5082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_64783, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000112 DASVTM010000000
VERSION     DASVTM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64783"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VF633_03260"
     CDS             <1..1320
                     /locus_tag="VF633_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8470108.1"
                     /translation="GVTLPIVKHSYLVRHAADIPAVIEEAFHIARSGRPGPVLIDLPK
                     DVQFEMTDAPRGFHLSNEIAVAEASVIAEAEAAIRAAKRPVLYIGGGAKISGAVDAVR
                     EFAAMTGIPQVSTLNALGTIPTGQDNFLGMLGMHGTRAANEAVQESDLLIVVGARFDD
                     RATGKLIEFAPLAGVVHFDIDPSEIGKLRKVAVGVVGDLKPNLEALNARLSTGPAIAV
                     SEWADRWRAKSIECAFDYNAPGDGVFAPQLLNELSKKAGPNFIAACDVGQHQMWVAQY
                     CEFSRPQAHITSGGLGSMGFGLPAGIGAKLAEPDAVVVTITGDGGVMMNIQELATVNR
                     FKIPLKIVLLDNSRLGMVRQWQEFFFERNFSEVDLSDNPDFVKVAEAFGIEAFRVDAR
                     DQVSAGIDRLLAAEGPILMHVIIDPNDNVWPLVPPGKNNAEMLEGAR"
     gene            1317..1571
                     /locus_tag="VF633_03265"
     CDS             1317..1571
                     /locus_tag="VF633_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8470109.1"
                     /translation="MTDTVHILIDRADGALQRLIGLVERRGFFIDGMNMEPEGAGLRV
                     SMTVRGRDAGRSMDNLGLQIDRLVGARRVSNDAAFRYEAA"
     gene            1575..3002
                     /gene="leuC"
                     /locus_tag="VF633_03270"
     CDS             1575..3002
                     /gene="leuC"
                     /locus_tag="VF633_03270"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX8470110.1"
                     /translation="MSVTTTSPKTLYEKVWDKHLVVAEAEATPGVLYIDLHLVHEVTS
                     PQAFSEIESRGLKVRRPDRTFATLDHSTPTLPAVNGLRPYVTDEARDQVSRLEKNCAA
                     HGVELAGWDSDARGVVHVMGPELGLTQPGMTVVCGDSHTATHGAFGALAFGIGTSEVG
                     HVLATQCLLQRASKTLRVTVDGELQPGVSGKDVALAVIAALGFGGGTGYVIEYAGSAV
                     TGLDMEGRMTLCNMSIEAGARAGMIAPDQKTIDWLRGRRKVPQGAAYDTAAAEWLALA
                     SDPGAVFDKEVVLDGSAIRPMATWGTTPDAGAPIGSKVPEPRSDSDRKAIAYMGFEAG
                     QTTTTQAVDVVFIGSCTNGRLPDLRAAAEVLKGRKVKDGVRMLVVPGSEAVRRDAEAE
                     GLDIIFIEAGAEWRIPGCSMCIAMNGDTVGPGQLAVSTSNRNFEGRQGKGARTILASP
                     ATAAASAIAGVLTDPRVYLSESVDA"
     gene            2995..3591
                     /gene="leuD"
                     /locus_tag="VF633_03275"
     CDS             2995..3591
                     /gene="leuD"
                     /locus_tag="VF633_03275"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX8470111.1"
                     /translation="MPEPIRVFTSRSLVLTQANIDTDQIIPARFLTTTETTGLGAKAF
                     YDWRYESDGSARPEAVLNRIDPAEHRILVAGDNFGCGSSREHAPWALYDYGFRAVIST
                     GIADIFTSNALKNGLLPIVVDQATWDDLVAHPEQPVTIDLELGEVRRGNHPPVTFTVE
                     AFARQCLLDGVDTLGWLQNKLPDIETYEREHAQAPEAV"
     gene            3591..4694
                     /gene="leuB"
                     /locus_tag="VF633_03280"
     CDS             3591..4694
                     /gene="leuB"
                     /locus_tag="VF633_03280"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX8470112.1"
                     /translation="MTTPKTYKIVLLPGDGVGPEVIAATKAVLQIIGDFYSHEFEFSE
                     HLIGGAAIDAVQNPLPQETIDACLASDGVLLGAVGGPKWDGQALRPEQGLLGIRKAMG
                     LFANLRPLQVSPVMAHRSPLKKEIVEGVDLIVFRELTGGVYFGDKGRKDDGSAYDVCS
                     YSIDEIERVTRAAFKIALQRRGKVTSVDKANVMETSRLWREVVTRIHAEEFPQVELKH
                     ALVDSMAMHLIQRPRDYDVILTENMFGDILSDEISVLGGSIGLLPSASLGASGPGLFE
                     PIHGSAPDIAGKELANPVGTILSAAMLLRHGLGLDDEAGSIEAAVAAVLAAGATTADL
                     GGELGTRAAAEAIMDAIRAIHWAAAHRVQMHWG"
     gene            4892..>5082
                     /locus_tag="VF633_03285"
     CDS             4892..>5082
                     /locus_tag="VF633_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX8470113.1"
                     /translation="MSQVLNRLPGILALLILTAVLFYAGGAFAQDAAPAAAPALLGHQ
                     VPLELNGADTSWLATSTALV"
BASE COUNT          860 a         1658 c         1683 g          881 t
ORIGIN      
        1 ggcgtcaccc tgccgatcgt gaaacactcc tatctggtcc gccacgccgc cgacattccg
       61 gcggtgatcg aggaggcgtt ccacatcgcc cgttcgggtc gtcccggtcc ggtcctgatc
      121 gacctgccca aggacgtcca gttcgagatg acggacgcgc cgcgcggctt ccacctttcg
      181 aacgagatcg ccgtcgccga agcctcggta atcgccgagg ccgaggccgc catccgcgca
      241 gcgaagaggc cggtcctgta catcggcggt ggggccaaga tctccggcgc ggttgatgcg
      301 gttcgcgagt tcgcggccat gaccggcatc ccgcaggtct cgaccctgaa cgctctgggc
      361 acgatcccga cgggtcagga caacttcctc ggcatgctgg gaatgcacgg cacccgcgcc
      421 gccaacgagg cggttcagga aagcgacctg ctgatcgtcg tcggcgcccg cttcgatgat
      481 cgcgcgaccg gcaagctgat cgagttcgca ccgctggccg gcgtcgtcca tttcgacatc
      541 gacccgtccg agatcggcaa gcttcgcaag gttgcggtcg gcgtcgtcgg cgacctgaag
      601 cctaatctgg aagcgttgaa cgcacgcctg tccaccggtc cggccatcgc ggtttcggaa
      661 tgggccgacc gctggcgcgc caaatcgatc gagtgcgcct tcgactacaa tgcgcccgga
      721 gacggcgtgt tcgcgccgca actgctgaac gagctgtcca agaaggccgg gccgaacttc
      781 atcgccgcct gcgatgtcgg ccagcaccag atgtgggtcg cgcagtattg cgagttctcg
      841 cgtccgcaag cccacatcac ctctggcggt ctgggctcga tgggctttgg cctgcccgca
      901 ggcatcggcg ccaagctggc cgagcccgac gctgtcgtcg tcaccatcac cggcgacggc
      961 ggggtgatga tgaatattca agaactggcc acggtcaacc gcttcaagat cccgctgaag
     1021 atcgtgctgc tggacaactc gcgtctgggc atggtccgcc aatggcagga gttcttcttc
     1081 gagcggaact tctccgaggt cgatctgtcg gacaaccccg acttcgtgaa ggtcgcggag
     1141 gcctttggca tcgaggcttt ccgcgtcgac gcacgcgatc aggtttccgc cggcatcgac
     1201 cgcctgctgg ccgccgaggg cccgatcctg atgcacgtca tcattgatcc caacgacaac
     1261 gtctggccac tggtgccgcc gggcaagaac aacgccgaaa tgctggaggg cgcacgatga
     1321 ccgacaccgt tcacatcctg atcgaccggg ccgacggcgc ccttcaacgg ctcatcggtc
     1381 tggtggagcg ccggggcttc ttcatcgacg gcatgaatat ggagccagaa ggtgcgggtt
     1441 tgcgggtctc gatgaccgtc cgtggccgcg acgccggtcg ctcgatggat aatctgggcc
     1501 tgcagatcga ccgtctggtc ggcgcgaggc gggtgtcgaa cgacgccgcc tttcgctacg
     1561 aggcagcctg accaatgagc gtaacgacta cctcccctaa gaccctgtac gagaaggtct
     1621 gggacaagca tctggtcgtg gccgaggccg aggcgacgcc cggcgtgctc tatatcgacc
     1681 tgcatctggt gcatgaggtc accagccctc aggccttctc ggagatcgag agccgggggc
     1741 tgaaggtccg acggccagat cgcacgttcg caacgctgga ccactcgacg ccgacgcttc
     1801 cggcggttaa cggcctgcgc ccctatgtca ccgacgaggc gcgggatcag gtgtcgcggc
     1861 tggagaagaa ctgcgccgcc cacggcgtcg aactggccgg gtgggattcg gatgcgcgcg
     1921 gcgtcgtcca cgtgatgggg cctgagctgg gactgaccca gcctggcatg acggtcgtat
     1981 gcggcgacag ccatacggcg acgcacggcg cctttggggc gctcgcgttc ggcatcggca
     2041 cgtccgaagt tggccacgtt ctggcgaccc agtgcctgtt gcagcgggcg tcgaagaccc
     2101 tgcgcgtcac ggtcgacggc gagttgcagc ccggtgtctc gggcaaggac gtggcgttgg
     2161 ccgtgatcgc cgcgctcggc tttggcggtg gcaccggcta cgtcatcgaa tatgcgggca
     2221 gcgccgtgac cggcctcgac atggaagggc ggatgaccct gtgcaacatg tcgattgagg
     2281 ctggtgcgcg cgcgggcatg atcgcgcccg accagaagac catcgactgg ctgcggggac
     2341 gccgcaaggt tccgcaaggc gccgcctatg acaccgccgc cgccgagtgg ctggcgctgg
     2401 cgagcgatcc cggcgcggtg ttcgacaagg aggtcgtgct ggacggctct gccatccgtc
     2461 cgatggccac ctggggcacg acgcccgacg cgggcgcccc catcggctcg aaggtgccgg
     2521 agccccgctc cgactccgac cgcaaggcca tcgcctatat gggtttcgag gccgggcaga
     2581 cgacgacgac ccaggccgtg gacgtggtct tcataggctc ctgcaccaac ggacgcctgc
     2641 ccgacctacg cgccgccgcc gaggtgctga agggccgcaa ggtcaaggat ggcgtacgga
     2701 tgctggtcgt cccgggctcc gaagcggtgc gtcgtgacgc cgaagccgag gggctggaca
     2761 tcatcttcat cgaggccggc gccgagtggc ggatcccggg ctgttcgatg tgcatcgcca
     2821 tgaacggcga caccgtcggg ccgggccagc ttgctgtctc gacctccaac cgcaatttcg
     2881 aaggccgtca gggcaagggc gcccgcacca ttctggccag ccctgcaacg gcggcggcct
     2941 cggccatcgc aggcgtgctg acggatccgc gcgtctatct gtcggagtcg gtcgatgcct
     3001 gagccgatca gagtattcac ctcgcgcagc cttgtgctga cccaagccaa tatcgacacc
     3061 gaccagatca ttccggcgcg cttcctgacc acgacggaaa cgacgggtct gggggccaag
     3121 gccttctacg actggcgcta tgaatccgac ggttcggcgc ggcccgaggc ggtgctgaac
     3181 cggatcgatc cggccgagca tcgcatcctc gtggcaggcg acaatttcgg ctgtggctcg
     3241 tcgcgcgaac atgcgccctg ggccctgtac gactatggct tccgggctgt gatctcgacc
     3301 gggatcgccg acatcttcac gtctaacgcc ctgaagaacg gcctgctgcc gatcgtggtg
     3361 gatcaggcga cgtgggacga tctggtcgcg cacccggaac agccggtgac cattgatctg
     3421 gagttaggcg aagtccggcg cggcaaccat ccgccggtga ccttcacggt ggaggccttc
     3481 gcccgccagt gcctgctgga cggcgtcgac acgctcggct ggctgcagaa caagctgcct
     3541 gatatcgaaa cctacgagcg cgaacacgcg caagccccgg aagccgtttg atgaccacgc
     3601 ccaagaccta caagatcgtc ctgctgcccg gcgacggcgt cggcccagag gtcatcgccg
     3661 cgaccaaggc ggtgctgcag atcatcggcg acttctacag ccacgagttc gagttttccg
     3721 agcatctgat cggcggcgcc gccatcgatg cggtccagaa cccgctgccg caggagacga
     3781 tcgacgcctg tctggcgtcg gacggcgtgc tgctgggcgc ggtcggggga ccgaagtggg
     3841 acgggcaggc gctgcgaccg gaacagggtc tgctgggcat tcgcaaggcc atgggtctgt
     3901 ttgcaaacct gcgcccgctg caggtctcgc ccgtcatggc gcaccgctcg ccgctgaaga
     3961 aggagatcgt cgagggcgtt gacctgatcg tcttccgcga gctgaccggc ggcgtctatt
     4021 tcggcgacaa gggacgcaag gacgacgggt ccgcctacga cgtctgttcc tattcgatcg
     4081 acgagatcga acgcgtcacc cgcgccgcct tcaagatcgc cctgcaacgt cgcggcaagg
     4141 tcacctcggt cgacaaggcc aacgtcatgg agacgagccg cctgtggcgc gaggtcgtga
     4201 cccgtatcca cgccgaggag ttcccacagg tcgagctgaa gcatgcgctg gtcgattcga
     4261 tggccatgca cctgatccag cgcccgcgcg attacgacgt catcctgacc gagaacatgt
     4321 tcggcgacat cctgtcggac gagatctcgg tgctgggcgg atccatcggc ctgttgccgt
     4381 cggcgtcgct gggtgcaagc gggccggggc tgttcgagcc gatccacggc tccgcgccgg
     4441 acattgcagg caaggagctg gccaaccccg tgggaacgat tctctcggcc gccatgctgc
     4501 tgcgtcacgg actgggtctg gatgacgagg ccggctcgat cgaggccgcc gtcgccgctg
     4561 ttctggctgc cggagcgacc accgccgacc tgggtggcga actgggcacg cgcgccgcgg
     4621 ccgaggctat catggacgcg attcgcgcca ttcactgggc ggcggcccac cgtgtgcaaa
     4681 tgcactgggg atgaggtgtg ctgttagcat ctgcgaaaca ggtatgaacc gttggcggaa
     4741 tgctaatacg cggcacaatc cttcatttgt tccgtgctga aataatacgc ctgcacgaaa
     4801 attgcgccgg gcacggttaa gcctcttgca tatgtccgtt ttgcgatggt tacttcgcag
     4861 ctgcgagact gcaacggggg atccaacgca catgtcacag gtactcaatc gacttccggg
     4921 catccttgcc ctgctgatac tcaccgccgt gctgttctat gcaggcggcg ccttcgctca
     4981 ggacgccgct ccggccgccg cgccggccct gctgggtcat caggttccgc tggaactgaa
     5041 tggcgctgac acgtcttggc tggcgacctc tacggcgctg gt
//
LOCUS       DASVTM010000113         5716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_328816,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000113 DASVTM010000000
VERSION     DASVTM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328816"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VF633_03290"
     CDS             <1..1133
                     /locus_tag="VF633_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8470114.1"
                     /translation="ELPENIEKAWRKVGRQGAKARKDWEARLAESKQAAEFTRAVIKG
                     DLPESAFDALNAKIAELIETKPAQATRQSSGAALDEVFAAIPELVGGSADLTGSNNTF
                     VKDTPIFDAPTYEGRYVNWGIREFGMAAALNGLALHGGIIPYGGTFMVFSDYSRPAIR
                     LGALMGVRAIHVLTHDSIGLGEDGPTHQPVEHLAALRAIPNLLVFRPADTVETMEAWK
                     IALEAKTMPSAMCLSRQKTPAVRTVGSDENLTARGAYEIKASNGEAKVTLFGTGTELA
                     LALKAAETLEAEGTPTRVVSVPCFELFEQQDAAYQASVIGRGTVRVAIEAAIKQGWER
                     FIGEDGSFIGMTSFGASAPADVLYREFGITTDAIVAAAKARL"
     gene            1138..1614
                     /locus_tag="VF633_03295"
     CDS             1138..1614
                     /locus_tag="VF633_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX8470115.1"
                     /translation="MTDVPSLYDWLGGREALLRLTTEFYRRVALDDVLSPVFAHMDAD
                     HPAHVADFIGEVLGGPALYDPSGHGHVRMIGHHLNKHLGETQRRRWFDLLLTTADDIG
                     LADDPEFRSAFVGYLEWGSRLAVINSQPGVKLPDSSPMPKWSWGAPGGPYIPDTSA"
     gene            1685..2521
                     /locus_tag="VF633_03300"
     CDS             1685..2521
                     /locus_tag="VF633_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470116.1"
                     /translation="MKTITSVAAAALILGLAACNPVGAAPTKVAEATETVSLVEKPKL
                     SEEVEALPRLVGDSPAILRINAELDRLDATATADAKECVEMAGDGPGGGWSRAITRPM
                     TGPAFVTMREHVEVYCGGAYPSTTQTAITYDLSTGARANWVALLPALALVQDEPMAEL
                     PADYIYSVRSPQLEALYERKMIADADDAEWRDGCREVWKPKLEDETGGQGFNVWADAV
                     HGGVAIDADYVHAVQACGGTVYLSADEMRAAGAPAGLINALTAAMAAGNWSPKEEAEA
                     AE"
     gene            2531..3727
                     /gene="purT"
                     /locus_tag="VF633_03305"
     CDS             2531..3727
                     /gene="purT"
                     /locus_tag="VF633_03305"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEX8470117.1"
                     /translation="MNLGTPLSDTAIRVMLLGAGELGKELAIELQRLGVEVIAVDRYA
                     NAPAMQVAHRSHIIPMTDPQVLNGVIMAEAPHIIVPEIEAIATEILAHIEAAGLAVVI
                     PTARAVQLTMNREGIRRLAAEELGLPTSPYAFAGSAAELAEAANTIGLPVFIKPVMSS
                     SGKGQSMIDALEDAGNAWAYAQSSGRVETTRVIVEGRIDFDFEITLLTVRSVGADGKI
                     RTDFCAPIGHRQVKGDYVESWQPQAMSPAALATSQDIAAKVTTSLGGLGVFGVELFVK
                     GDEVWFSEVSPRPHDTGLVTLATQTMSEFALHARAILGLPVDVSQRDIGASAVIYGGM
                     DAVGIAFEGVAEALAVPTADLRLFGKPESFERRRMGVATAWGATVEEARERATLAASL
                     VRPVRS"
     gene            3865..4872
                     /gene="gap"
                     /locus_tag="VF633_03310"
     CDS             3865..4872
                     /gene="gap"
                     /locus_tag="VF633_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX8470118.1"
                     /translation="MTLRVAINGFGRIGRLVLRSIVEHGRTDIEVVSINDLGPVETNA
                     HLFRYDSVHGRFPGTVTTGEDWIDVGTGKIRVTAERDPANLPHAELKVDIAFECTGIF
                     TSKDKASAHLKAGAKRVLVSAPADGSDKTIVYKVNHETLTADDIVVSNGSCTTNALAP
                     VAKVLNDLFGIERGYMTTIHSYTGDQPTLDTMHKDLYRARAAALSMIPTSTGAAKALG
                     LVLPELKGKLDGSSIRVPTPNVSVVDLKVVSGRDVTVEEINAALQAAADGPMKGVLAT
                     TIEPLVSHDLNHIAASSTAALPQTQVVDGKLARVLTWYDNEWGFASRMADTAMVMAKF
                     I"
     gene            5169..>5716
                     /gene="pgk"
                     /locus_tag="VF633_03315"
     CDS             5169..>5716
                     /gene="pgk"
                     /locus_tag="VF633_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX8470119.1"
                     /translation="MTFRTLDDAGSLAGKTALVRVDFNVPMEDGRITDDTRLRVALPT
                     INKLREAGAKVALLAHFDRPKGQVVPSMSLKPLVGPLSELLGAPIHFAEDCVGGPAHA
                     AIDGLDAGGVVLLENVRFHAAEEANDAEFATSLSELADLFVNDAFSAAHRAHASTEGV
                     AHHLPAYAGESMRRELEALDAAL"
BASE COUNT         1036 a         1903 c         1817 g          960 t
ORIGIN      
        1 tcgaactgcc cgagaacatc gagaaggcct ggcgcaaggt cggtcgtcag ggcgccaagg
       61 ctcgcaagga ctgggaagcc cgtctggcgg aatccaaaca ggctgcggag ttcacccgcg
      121 cagtcatcaa gggcgacctg ccagaaagcg ccttcgatgc gttgaacgca aagatcgctg
      181 agctgatcga gaccaagcct gcgcaggcca cgcgccaatc gtcaggcgcg gcgctcgatg
      241 aagtcttcgc cgcaatcccc gaactggtcg gcggctcggc cgatctgacc ggctcgaaca
      301 acaccttcgt caaggacacg ccgatcttcg acgccccgac ctatgaaggc cgctacgtga
      361 actggggcat ccgcgagttc ggcatggctg ccgctctgaa cggtctggcc ctgcacggcg
      421 ggatcatccc ctacggcggc accttcatgg tgttctcaga ttacagccgc ccggcgatcc
      481 gcctcggcgc cctgatgggg gtgcgcgcca tccacgtgct gacccacgat tcgatcggtc
      541 tgggcgaaga cggcccgacg catcagcccg tcgagcatct ggccgccttg cgcgccatcc
      601 cgaacctgct ggtcttccgt cccgccgata cggtcgaaac gatggaagcc tggaagatcg
      661 cgcttgaggc gaagaccatg ccctcggcca tgtgcctgtc gcgccagaag accccggcgg
      721 tccgcaccgt ggggtccgat gagaacctga ccgctcgcgg cgcctacgag atcaaggctt
      781 ctaacggcga ggccaaggtc accctgttcg gcacaggaac cgaactggcg ctggcgctga
      841 aggctgccga aacgctggaa gccgaaggca cgccgacgcg cgtcgtctcg gtcccctgct
      901 tcgaactgtt cgagcaacag gacgccgcct atcaggcctc ggtcattggc cgcggcacag
      961 ttcgcgtagc gatcgaagcc gcgatcaaac agggctggga acggttcatc ggcgaagacg
     1021 gatccttcat cggcatgacc agcttcggcg cctcggctcc ggcggacgtg ttgtaccgcg
     1081 agttcggcat cacgacggac gcgatcgtgg cggcggccaa ggcgcggctc taggaggatg
     1141 accgatgtcc caagccttta cgactggctg ggaggtcgcg aggccctgct gagactgacg
     1201 accgaattct atcgccgcgt ggcgctggat gacgtcctgt cgccggtatt cgcccacatg
     1261 gacgccgacc atccggccca tgtcgcagac ttcatcggtg aggtgctggg cggaccggcg
     1321 ctctatgatc catcgggcca cggccatgta cggatgatcg ggcatcattt gaacaagcat
     1381 ctcggcgaaa cgcagcgacg gcgctggttc gacctgttgc tgaccaccgc ggatgatatc
     1441 ggtctggccg acgacccgga gttccggtcg gccttcgttg gttacctgga atggggatcg
     1501 cggcttgcgg tgatcaactc gcagccgggc gtcaaactgc cggactcctc gccgatgccg
     1561 aaatggagct ggggcgcgcc gggtgggccc tatattccgg acacctcggc ctgagacatt
     1621 ttcagtggct ggcgagatcg acggatcggg agcaagcttg ccgcgatttc agggggcttt
     1681 gcagatgaag acgatcacga gtgtggcggc agcggcgctg attctgggac tcgcagcgtg
     1741 caatccggtt ggagccgcgc cgaccaaggt cgccgaggct accgaaaccg tatccctggt
     1801 cgagaagccc aagctttccg aggaggtgga agccctgccc cggctggtcg gcgacagtcc
     1861 tgcgatcctg cgcatcaacg ccgagctgga tcggctggac gccacagcga cggcggacgc
     1921 gaaagagtgc gtcgagatgg ccggagacgg accgggaggc ggctggagcc gcgcgatcac
     1981 gcgaccgatg accggtccgg cctttgtgac catgcgcgag catgtcgagg tctactgcgg
     2041 cggcgcctat ccgtcgacca cacagacggc catcacctac gacctgtcga ccggggcacg
     2101 cgccaactgg gtggccctgc tgccggctct ggccctggtt caggacgagc ccatggccga
     2161 gttgcccgcc gattatatct acagcgtccg ttcgcctcag ctggaagctc tgtacgaacg
     2221 caagatgatc gccgacgccg acgacgccga gtggcgcgac ggatgccgcg aggtatggaa
     2281 gccgaagctg gaggatgaga cgggcggcca gggcttcaac gtgtgggccg atgccgtaca
     2341 cggcggggtg gcgatcgacg ccgactatgt ccacgcggtg caggcctgcg gaggaaccgt
     2401 ctatctgtcg gccgacgaga tgcgcgcggc cggcgcgccc gcgggtctga tcaacgccct
     2461 gaccgctgca atggcggctg ggaactggtc gcccaaggaa gaagccgaag cggctgaata
     2521 ggaaaacatg atgaacctcg gcaccccgct ttccgacacc gcgatccgcg tcatgctgct
     2581 gggcgcaggc gagctcggca aggaactcgc catcgaactg cagcggctcg gcgtcgaggt
     2641 gatcgcggtc gatcgctacg ccaacgcacc ggccatgcag gtggcgcatc gctcccacat
     2701 catcccgatg accgaccccc aggtgctgaa cggcgtcatc atggccgagg ctccgcacat
     2761 catcgtgccc gagatcgaag ccatcgccac cgagatcctc gctcacatcg aagcggcagg
     2821 tctggccgtg gtcatcccga cggcgcgcgc tgtgcaactg accatgaacc gcgagggcat
     2881 ccgccggttg gcggccgaag agctcggcct gccgaccagc ccctacgcct ttgccggaag
     2941 cgcggcggaa ctggccgagg cggcgaacac catcggcctg cccgtcttca tcaagccggt
     3001 gatgtcgtcg tcgggcaagg gccagtcaat gatcgatgcg ctcgaggatg cgggtaacgc
     3061 ctgggcctat gcgcaatcca gcggccgggt cgagacgacg cgagtcatcg tcgagggccg
     3121 gatcgatttc gacttcgaga tcacgctgct aacggtccgc tcggttggag ccgacggcaa
     3181 gatccgcacc gacttctgcg cgcccatcgg tcaccgtcag gtcaagggcg actatgtcga
     3241 gagctggcag ccgcaggcca tgagcccggc ggctctggcc acgtcacagg atatcgcggc
     3301 gaaggtcacc acctcgttgg gggggcttgg cgtgttcggc gtcgaactgt tcgtaaaggg
     3361 tgacgaggtc tggttctccg aggtctcgcc acgtccgcac gacaccggac tggttaccct
     3421 cgccacccag accatgagcg agttcgccct gcacgcgcgc gcgatcctcg gcctgccggt
     3481 cgacgtcagc cagcgcgaca tcggcgccag cgcagtcatc tatggcggaa tggacgccgt
     3541 cggcatcgcc ttcgagggcg tcgcagaggc cttagccgtc ccgaccgccg acctgcgcct
     3601 gtttggaaag cccgaaagct tcgaacgccg ccgcatgggc gtggccacgg cgtggggcgc
     3661 gaccgtcgag gaagcgcgcg aacgggccac tctcgccgca agtctggtca ggccggtccg
     3721 gtcgtaggca gccacacacg cagcaacgcc cttcaaagcg tcacagttca tcacgcgatg
     3781 ttacgcgtgc gggggttgat cctcgcgggc gtcggctcca ttgaagcgtc aactctagac
     3841 accacgcctt tcaggagccc gctcatgacc cttcgcgtcg ccatcaatgg ctttggccgc
     3901 atcggccgtc tcgtcctccg ctcgatcgtc gagcatggcc gcacggacat cgaggtcgtg
     3961 tcgatcaacg atctgggccc ggtcgagacc aatgctcacc tgttccgcta tgactcggtc
     4021 cacggtcgct tccccggcac ggtgacgacg ggcgaagact ggatcgatgt cggcaccggc
     4081 aagatcaggg tcaccgccga gcgcgatccc gccaacctgc cgcatgccga gctcaaggtc
     4141 gatatcgcct tcgaatgcac ggggatcttc acgtccaagg acaaggcctc ggctcacttg
     4201 aaggcgggcg ccaagcgcgt gctcgtgtcc gcgcctgcgg acggttccga caagaccatc
     4261 gtctacaagg tcaaccatga gaccctgacc gccgacgaca tcgtcgtctc gaacggctcc
     4321 tgcaccacca acgctctggc tcccgtcgcc aaggtgctga acgacctgtt cggcatcgag
     4381 cgcggctaca tgaccaccat ccactcctac accggcgacc agccgacgct ggatacgatg
     4441 cacaaggacc tgtaccgcgc ccgcgctgca gccctgtcga tgatcccgac ctcgaccggc
     4501 gccgccaagg ctttgggtct ggttcttcct gaactgaagg gcaagctgga cggctcctcg
     4561 atccgcgtcc cgaccccgaa cgtctcggtc gtcgacctga aggttgtctc cggtcgtgat
     4621 gtcacggtcg aggaaatcaa cgccgcgctt caggccgccg ccgacggtcc gatgaagggc
     4681 gttctcgcca ccacgatcga gcctttggtc tcgcacgacc tgaaccacat cgccgcctcc
     4741 tccaccgccg ccctgccgca aacccaggtc gtcgacggca agttggcccg cgtcctgacc
     4801 tggtacgaca atgagtgggg cttcgcctcg cgcatggccg acaccgccat ggtgatggcc
     4861 aagtttatct gatgagcgtg gaggcggaca tccatttacg gttgtccgcc cagcgcgcgt
     4921 tgcttggcgg tgacgctagg cctgaggtcg ttttccgtgg agctgaagtc tggcgtagtt
     4981 cacacgctgg ctgtctatgc gaatgagccg accgaagttg agaccgatct gattcaggcc
     5041 gctgtgacgg aaatcatcgc cggttatctt gttgagacac tcgacgaaca gattgtggtc
     5101 agttcaaaaa agccacttcc tcgattgacc cacgtcgcct ttgaacgaca cgagccataa
     5161 ggacccccat gaccttccgc accctcgacg acgccggctc cctcgcgggc aagaccgcct
     5221 tggtccgcgt ggatttcaac gtgccgatgg aagacgggcg gatcacagac gacacccgcc
     5281 tgcgggtcgc cctgccgacg atcaacaaac tgcgtgaagc cggtgccaag gtcgcacttc
     5341 tggcccattt cgaccgcccc aagggtcagg tcgtgccgtc gatgagcctg aagcctctgg
     5401 tgggtccgct gtccgaactt ctgggtgctc cgattcattt cgctgaggac tgcgtcggag
     5461 ggccagccca cgccgccatc gacggactgg atgcaggcgg cgtcgtcctg ctcgaaaacg
     5521 tacgcttcca cgccgccgag gaagccaacg acgccgagtt cgcgaccagc ctgtccgaac
     5581 ttgccgacct ctttgtcaac gacgccttct ccgccgctca ccgggcccac gcctcgaccg
     5641 aaggcgtcgc gcaccacctg cccgcctatg ccggcgaatc catgcggcgc gagctggagg
     5701 cgctagacgc cgcgct
//
LOCUS       DASVTM010000114         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_914713,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000114 DASVTM010000000
VERSION     DASVTM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914713"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF633_03320"
     CDS             complement(<1..400)
                     /locus_tag="VF633_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470120.1"
                     /translation="MTEEADIVPEPEAAAAESSAKVKKPKRSLLRRIGVGVGIAVAAL
                     IVLLLVATVGGRMYLTSDAGRGEVVKALTDLRLSRYGRLKVEGLSGDLFSDFKVARLT
                     VSDAKGVWLEANDLRVDWKFSTLLGRRFHAN"
     gene            complement(397..>1588)
                     /locus_tag="VF633_03325"
     CDS             complement(397..>1588)
                     /locus_tag="VF633_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8470121.1"
                     /translation="TEGSTNPDWVAGLAPWKEGDKFEPDKVAELERRLLDTGVYDGVG
                     VALAPADQTNAGGNRPVVVTLNDRPRRILEAGASFSTAEGSGVDAIWTWYNHFNRADT
                     LRFQARAANIDSRLGVDLSLPHWRRPGQTLKLSTAVVNEDTDAYRRTALTAAADLQQR
                     IGRTSYYSYGVAIDAGQYAENRFDPLTQLPVGLDRDLVILTGRGNAYLDESNDPLNPT
                     KGYRLTLNVQPTAVAGEDTVLFLRTETQGTMYVPIEKTGHTILAGRVRLGAILGGQEL
                     TVPADRLFYSGGGGSVRGYEYQGVNPRLPDNTPRGGLSLFETSLEVRRDIGESWQVVG
                     FVDAGAIGFQETPNLTNMRYAAGIGGRFKLPFGPVRADLAFPLDPREGDSTFQVYISI
                     GQAF"
BASE COUNT          275 a          534 c          506 g          273 t
ORIGIN      
        1 cgttggcgtg gaagcgacgc ccaagcaggg tcgagaattt ccagtcgaca cgaaggtcat
       61 tggcctccag ccagacgccc ttggcgtccg aaaccgtgag acgcgccacc ttgaagtcgc
      121 tgaacaggtc cccgctcaac ccctcgacct tcaaccggcc atagcggctc agcctgaggt
      181 ccgtcagcgc cttgaccact tcgccacgtc cggcgtccga cgtcaggtac attcgtccgc
      241 cgacggtcgc gaccaacagc agcacgatca gcgccgcgac ggcaatcccg acgccaacac
      301 cgatccgacg cagcagcgaa cgcttcggct tcttcacctt cgcagacgat tcggcggcag
      361 ccgcttcggg ctcgggaacg atatcggcct cttcggtcaa aacgcctgac cgatgctgat
      421 gtagacctgg aaggtgctgt cgccctcacg cggatcgagc gggaaggcga ggtcggcgcg
      481 aaccgggccg aagggcagct tgaaccggcc gccgatccct gccgcatagc gcatgttggt
      541 caggttcggc gtttcctgaa agccgatcgc gcctgcatcc acaaatccga ccacctgcca
      601 gctctcgccg atgtcgcgcc ggacctcaag cgatgtctcg aatagcgaca gcccgccgcg
      661 cggggtgttg tcgggcagtc gaggattgac gccctgatac tcgtaaccgc gcaccgaacc
      721 gccgccaccg gaatagaaca gacggtctgc agggacggtc agttcctgac cgccgaggat
      781 cgcacccagc cggacccgac ctgccagaat ggtgtggccg gttttttcga tcggaacgta
      841 catggtccct tgcgtctcgg tgcgtaggaa cagcaccgta tcctcgcccg ccaccgccgt
      901 cggctggacg ttcagggtaa ggcgatagcc cttggtcggg ttcagcgggt cgttcgactc
      961 gtccagatag gcgttgcccc ggccggtcag aataacgagg tcgcgatcga gtccgaccgg
     1021 cagttgggtc aacggatcga aacggttctc ggcatactgg ccggcgtcga tcgccacgcc
     1081 atagctgtaa tacgacgtcc gtccgatgcg ctgctgcagg tcggcggcgg ccgtcaacgc
     1141 agtgcgccga taggcgtcgg tgtcctcgtt gactaccgct gtcgacagct tcagcgtctg
     1201 ccccggacga cgccagtggg gcagggacag gtcgacgccc agacgactgt cgatattggc
     1261 ggcccgcgcc tggaatctca gcgtgtcggc gcggttgaag tggttgtacc aggtccagat
     1321 ggcgtccacg ccggagcctt cggcggtcga gaagctggcc ccggcctcga ggatccggcg
     1381 cggccggtcg ttcagcgtga cgacgaccgg gcgattgccg ccggcgttgg tctggtcggc
     1441 aggcgcaagt gcgacgccca ccccgtcata gacgccggtg tcgagcagcc tgcgctccag
     1501 ttcggcgacc ttgtcgggct cgaacttgtc cccttccttc cagggagcca ggcccgccac
     1561 ccagtccggg ttagtcgacc cttcggtg
//
LOCUS       DASVTM010000115         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_436485,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000115 DASVTM010000000
VERSION     DASVTM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436485"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VF633_03330"
     CDS             complement(<1..489)
                     /locus_tag="VF633_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX8470122.1"
                     /translation="MKRVRLDQLLVTRGLSESRSKARAAIEAGGVTVDGQPARSASQQ
                     VERDADIVAVEAHRFVGRGALKLDHALTLWPVLVEGRVVLDVGASTGGFTEVCLERGA
                     VKVFAVDVGSGQMHERVAADPRVVNLEKTDARSLDTDLIPETPELIVCDASFIGLAKV
                     LPT"
     gene            complement(486..>2025)
                     /locus_tag="VF633_03335"
     CDS             complement(486..>2025)
                     /locus_tag="VF633_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8470123.1"
                     /translation="PAIKAVETGATVFEPKSYEKIYFEWLRNIEPWCISRQLWWGHRI
                     PAWYDADGQIYVAETAAEAVALAGGKALTQDEDVLDTWFSSALWPFSTMGWPEKTSDL
                     EKFYPTSDLVTAADIIFFWVARMMMMGLHFMDEVPFKRVIINGLVRDDKGQKMSKSKG
                     NVIDPLVIIDELGADPLRFTMAILSGTRDIKLSKERIEGYRNFGTKLWNAARFSQMNE
                     AVRVEGFDPATATQTINRWIRGELTKAERQVSEAIEGGRFDDAASALYRFVWNVFCDW
                     YVELSKPVLQGADEAAKAETRAMTAWTLDQTLKLLHPVMPFITEELWDELGKEGPTRD
                     EAMLISAKWPVLPDAFLDAEAEAEIGWLVDLITEVRQLKAEMNVPQGAKPPLAFVAPD
                     AVTAGRITLHQPLILTLARVSEVSVVEVAPPGAVTFVTGGATAAISLAGTIDVAAEKA
                     RLGKAVVAATSDVDHINKKLGNPNFVAKAAPAVIEEQRAKLAEAEEAKAKLVAALERL
                     GSLE"
BASE COUNT          344 a          664 c          641 g          376 t
ORIGIN      
        1 ggtcggcaag accttggcca gaccgatgaa gctggcgtcg cagacgatca gttcgggcgt
       61 ttcggggatc aggtcggtgt cgaggctgcg ggcgtcggtc ttctccagat tgaccacgcg
      121 aggatcggcc gcgacgcgct cgtgcatctg gccggaaccc acgtcgacgg cgaacacctt
      181 caccgctccg cgttcgagac acacctcggt gaagccgccg gtcgaggcgc cgacgtccag
      241 caccacccga ccctcgacca agacgggcca gagggtcagg gcgtggtcga gcttgagcgc
      301 gccccgtccc acgaagcgat gcgcctcgac ggcgacgata tcggcatcac gttcaacctg
      361 ctgcgaggcg gatcttgccg gctgaccatc gacggtgacc ccgcccgcct cgatggccgc
      421 gcgcgccttc gaccggctct cggacaggcc gcgcgtgacc agcaactggt ccagtctgac
      481 gcgcttcatt ccagcgatcc cagacgctca agcgccgcga ccagtttggc tttcgcttcc
      541 tcggcctcgg cgagcttggc gcgctgctcc tcgatgacgg cgggcgcagc cttggcgacg
      601 aagttcggat tgccgagctt cttgttgatg tgatcgacgt cggaggtcgc agccacgaca
      661 gccttgccca aacgcgcctt ctcggcggcc acgtcgatgg tcccggccag cgatatcgcc
      721 gccgtggccc cgccggtgac gaaggtcacg gcgcccggcg gcgccacctc gaccacggag
      781 acttcggaca cgcgggccaa ggtcaggatc aatggttgat gcagcgtgat ccgtcctgcc
      841 gtcacggcgt caggagcgac gaaagccagc ggtggtttcg cgccctgcgg cacattcatc
      901 tcggccttga gctgacgcac ctcagtgatc aggtcgacca gccagccgat ctcggcctcg
      961 gcctcggcat cgaggaaggc gtcgggcagg accggccatt tggcgctgat cagcatggct
     1021 tcatcgcgcg tggggccttc cttgccgagc tcgtcccaca gctcttcggt gatgaagggc
     1081 atgacggggt ggagcagctt cagcgtctgg tcgagcgtcc aggcggtcat ggcgcgcgtt
     1141 tcggccttgg cggcttcgtc cgcaccttgc agcacaggtt tggacagttc gacgtaccag
     1201 tcgcagaaga cattccagac gaagcggtag agcgccgagg cggcgtcgtc gaaacggccg
     1261 ccttcgatgg cttcggacac ctgacgctcg gccttggtca gctcgccacg gatccagcgg
     1321 ttgatggtct gggtcgcagt cgcgggatcg aagccctcca cgcgtaccgc ctcgttcatc
     1381 tggctgaagc gtgcggcatt ccacagcttg gtgccgaaat tacgatagcc ttcaatgcgt
     1441 tctttcgaca acttgatgtc gcgtgtgccc gacaggatgg ccatggtgaa gcgcagcggg
     1501 tctgcgccca gctcatcgat gatcacaaga ggatcgatca cgttcccctt ggatttgctc
     1561 atcttctggc ccttgtcgtc gcggacgagg ccattaatga tgacgcgctt gaacgggacc
     1621 tcgtccatga agtgcaggcc catcatcatc atccgggcga cccagaagaa gatgatatcc
     1681 gccgccgtca ccaggtccga ggtcgggtag aatttctcca gatccgacgt cttctccggc
     1741 caccccatcg tcgagaacgg ccacagcgcc gacgagaacc aggtgtcgag aacgtcttcg
     1801 tcctgggtca gggccttgcc gccggccaga gccacggcct ccgctgcggt ctccgcgacg
     1861 tagatttggc cgtcggcatc ataccatgcc gggatgcggt gaccccacca gagctggcgc
     1921 gagatgcacc atggttcgat gttgcgcagc cattcgaaat agatcttttc gtacgatttc
     1981 ggctcgaaca ccgtcgcacc ggtctcgacc gccttgatgg cgggt
//
LOCUS       DASVTM010000116         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_490036,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000116 DASVTM010000000
VERSION     DASVTM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490036"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..715
                     /gene="phnC"
                     /locus_tag="VF633_03340"
     CDS             2..715
                     /gene="phnC"
                     /locus_tag="VF633_03340"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX8470124.1"
                     /translation="MEVAAGEMVALIGPSGSGKSTLLRSITGMHPIDPGKGTIQVFGE
                     TVQKNGRTTSQIRAARAKLGMIFQQFNLVGRLSLFSNVMLGTLGRLPGWRGMLGAWPQ
                     ADKDKAMAALARVGVADYAAQRANTLSGGQQQRGAIARAIVQGAKAVIADEPVASLDP
                     VSARKVMELLVELNRRDGLGVIVTLHQVDYAIRYCERVIALKAGKIVYDGPSTGLDTK
                     TLIDIYGPEFEDAFWEAKA"
     gene            712..>1698
                     /gene="phnD"
                     /locus_tag="VF633_03345"
     CDS             712..>1698
                     /gene="phnD"
                     /locus_tag="VF633_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8470125.1"
                     /translation="MSRTLLTPRPLLGRVLGIAAAAVMTLGLASCGGGDDKKAGAAPT
                     EITFSILSAEGQASAGPLWQPLLDDMTKATGVTVKPFFGSNYTVLVEAMRGGQTQVGW
                     FSAKPALEAVDRANAEVIARTVNKEGLDSYRATLIARKDSGITLDQVLACGKKYDFGI
                     GDAQSTSGTLAPMSFLFNPRGIVPANCFKTVRSANHQANAFSVATGVLDVATSNSVNT
                     VFMTRENPTMAAQIHDIWQSPPIPESGIVVSETLPPALKEKVRTFFLSYGKGTGVEAE
                     RQRQVLASLEYSEFRAADDGYLDPIRVMVADQKLSDAKAKGNAAAVTAAEAEL"
BASE COUNT          295 a          617 c          515 g          271 t
ORIGIN      
        1 ggtggaggtc gccgccggag agatggtggc ccttatcggc ccctcgggct ccggcaagtc
       61 cacgctgttg cgctccatca ccggcatgca ccccatcgat cccggcaagg gcacgatcca
      121 ggtgttcggc gagacggtac agaaaaacgg tcgcacgacc agccagatcc gcgccgcccg
      181 cgccaaactt ggcatgatct tccagcagtt caatctggtc ggccggctgt cgctgttctc
      241 gaacgtcatg ctcggcacgc tcggtcgtct gcccggctgg cgcggcatgc tcggcgcctg
      301 gccccaggcg gacaaggaca aggccatggc ggccctcgcc cgcgtcggcg tcgccgacta
      361 cgccgcccag cgcgccaaca ccctgtcggg cggccagcag cagcgcggcg ccatcgcccg
      421 cgcgatcgtt cagggcgcca aggcggtcat cgccgacgag ccggtcgcct cgctcgaccc
      481 cgtgtccgcc cgcaaggtca tggaactgct tgtcgagctc aaccggcgtg acggtctcgg
      541 cgtcatcgtc accctgcacc aggttgacta cgccatccgc tactgcgagc gcgtgatcgc
      601 gctgaaggcg ggcaagatcg tctatgatgg cccatcgacg ggcctcgaca ccaagaccct
      661 tatcgacatc tacggtcccg agttcgagga cgcgttctgg gaagccaagg catgagccgc
      721 accctcctta ctccccgtcc tcttctgggc cgagttctcg gcatcgccgc agccgccgtc
      781 atgacgctgg gcctcgcctc ctgtggtggc ggcgacgaca agaaggccgg cgcagccccg
      841 accgagatca ccttctcgat cctgtccgcc gaaggtcagg cctcggccgg cccgctgtgg
      901 cagccgctgc tcgacgacat gaccaaggcg accggcgtca cggtcaaacc gttcttcggc
      961 tcgaactaca ccgtgctggt cgaagccatg cgtggcggcc agacgcaggt cggctggttc
     1021 tcggccaagc ccgcgcttga agccgtcgat cgcgccaacg ccgaggtcat cgcccgtacg
     1081 gtcaacaagg aaggcctcga cagctaccgg gccaccctga tcgcgcgcaa ggactcgggc
     1141 attacgctgg atcaggttct ggcctgcggc aagaaatacg acttcggcat cggcgacgcc
     1201 caatcgacct cgggcacgct ggccccgatg tccttcctgt tcaatccgcg cggcatcgtt
     1261 ccagccaact gctttaagac ggtccgctcg gccaaccatc aggcgaacgc cttcagcgtc
     1321 gcgaccggcg tgctggacgt cgccacctcg aacagcgtca acacagtctt catgacgcgt
     1381 gaaaacccca ccatggccgc ccagatccac gacatctggc agtccccgcc gatcccggag
     1441 tcgggcatcg tcgtctccga gaccctgccc cccgcgttga aggaaaaggt ccgcaccttc
     1501 ttcctgtcgt acggcaaggg cacaggcgta gaggccgaac gtcagcgtca ggtcctggcc
     1561 agccttgaat attccgagtt ccgcgccgcc gacgacggct atctcgaccc gatccgggtg
     1621 atggtcgcgg accagaaact ttccgacgcg aaggccaagg gcaacgccgc cgccgtgacc
     1681 gccgccgagg ccgaactg
//
LOCUS       DASVTM010000117         5124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_649076,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000117 DASVTM010000000
VERSION     DASVTM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649076"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1319
                     /locus_tag="VF633_03350"
     CDS             168..1319
                     /locus_tag="VF633_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8470126.1"
                     /translation="MPPEIQTLTREIAALWRQFNWLPEWAVIAIVLAVFVGGGSIVHE
                     IVFSVLRRVAKNRDVFWRGVVERGRAKLRVMMIIIAIGIGVTVSPMDPGPSLQIRSVL
                     VFVFILTLGWLASGVLDMWTAMHLRRFNMAAEDNLIARKHYTQARILQGVAKVLLVII
                     TVGLALMTIAGFRQWGVSLLASAGVVGIIAGLALQPVLTNLLAGIQIALTQPIRIDDA
                     VIVENEWGNVEEITATYVVVKLWDWRRMVLPLSWFITKPFQNWTRENARLIGTAMFYV
                     DYEAPIDKLRAGFEAIVKNSKLWDGDVQVMQVTDITERVMQVRCLASARSAPVAFDLR
                     CEIREKLMAFMRDECPEALPRDRVDLERISAGPDRPPEPLDPTTPPPQA"
     gene            complement(1255..1734)
                     /locus_tag="VF633_03355"
     CDS             complement(1255..1734)
                     /locus_tag="VF633_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470127.1"
                     /translation="MLRIALAVAAMACALPATAQTTAPAEHRFLLTGAGSFAVFADLT
                     TIQRAGDIATLRSFQVVEPGFAVGGTAYWGGWSRWSFDCKAMTADRLDFASVREGGVE
                     GPSSPDNQPAYSAGPGGDAAELLAVACGDPDRPADASTLEAAVSLGRAALAGDLDPH"
     gene            complement(1740..2558)
                     /gene="map"
                     /locus_tag="VF633_03360"
     CDS             complement(1740..2558)
                     /gene="map"
                     /locus_tag="VF633_03360"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX8470128.1"
                     /translation="MYETPELETETFTRSNAIRIYDQDDFEGMRIAGRLVAEALDMIT
                     PFVVPGVTTGELDDRIREYTIERGGLPACLGYRGYTKTVCTSINHVVCHGIPGDRVLK
                     DGDIVNIDHTVIVDGWHGDSSRMYSVGNINARTKKLVDVTYESLMLGLEQVKPGNTFG
                     DIGYAIQRHVEAARMSVVRDFCGHGIGRVFHDSPNVLHYGRKGEGAVLKPGMCFTIEP
                     MVNLGKPHVKVLSDGWTAVTRDKSLSAQCEHTIGVTETGVEIFTASPAGLFRPN"
     gene            complement(2651..3037)
                     /locus_tag="VF633_03365"
     CDS             complement(2651..3037)
                     /locus_tag="VF633_03365"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8470129.1"
                     /translation="MAKPAPTVTVWLDSLDQPTRAAIDALREVVRNSGGALHEEIKWN
                     APNYAHEGRDRVTLGIEPRGGYRVVLHRGAKVADTAGFHFEDPDRLAVWPSPDRGVVR
                     LADRAEIEAKCEALAGLIARWIVATA"
     gene            complement(3081..3758)
                     /locus_tag="VF633_03370"
     CDS             complement(3081..3758)
                     /locus_tag="VF633_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8470130.1"
                     /translation="MKKAIVTGGAGLIGSGICQTLAANGWQVASFDLKPGPSEARSIM
                     CDIADEASVAAAFAELGWSELELLVNNAGVANATSGSIADLSLADWRKVTDSHLTGAF
                     LMTRAAVPLLGEGSSVVNMVSTRAFMSEPDSEAYAASKGGLVALTHALAISLGPRVRV
                     NAIAPGWISDGKDLRDVDHEQHPVGRVGEAPDIADAVVYLAGAGFVTGQVLTVDGGMS
                     RKMIYEG"
     gene            3850..4281
                     /locus_tag="VF633_03375"
     CDS             3850..4281
                     /locus_tag="VF633_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470131.1"
                     /translation="MPLIQIMIQLRDKERFPNPVAIAALAERFNLPNDSNMQDWEYVV
                     ADLARLDEFLAYAESCPDEDEAFVLIKLIFQSFEDLGGTALEDPRWARVDKLLNTDFE
                     LHLSTLRYWASADQISEDGWHIAPLVRSVIASHGVEKLSPS"
     gene            4445..4822
                     /locus_tag="VF633_03380"
     CDS             4445..4822
                     /locus_tag="VF633_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470132.1"
                     /translation="MDQRIRNRIMEELLGLAEGDENIRQIGAGEYFEAFYDWVPHRGD
                     GIRPNGALTQDEAALILNVSIALDEAWDATPGMVSAEELIATGWPERIKPLAQSALDQ
                     MLKRGRFSEDAEEDEPSSAIPWP"
     gene            complement(4875..>5124)
                     /locus_tag="VF633_03385"
     CDS             complement(4875..>5124)
                     /locus_tag="VF633_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecF"
                     /protein_id="HEX8470133.1"
                     /translation="LANPAPVCVLDEVDAPLDDANVDRFCRMLNEMRNRTDTRFIVIT
                     HNPVTMSRMDRLYGVTMPERGVSQLVSVDLHQAEKLVA"
BASE COUNT          907 a         1528 c         1730 g          959 t
ORIGIN      
        1 ggactgttct gtcttagtct gatccgggcc gaggctcact ttcaccatca cgcccctcac
       61 cctttcgcgc gaaggctgat cgctgcgctc tcaggcgctc aagccctctc ccggtgggag
      121 agggtaggtt aatccgaaca accttggcct tggcgcgttc tagtcccatg cctccggaaa
      181 tccagacact gacccgcgag atcgctgcgc tgtggcgaca gttcaactgg ctaccagaat
      241 gggctgtgat cgccatcgtt ttggcggtgt tcgtcggagg cggatcgatc gttcacgaga
      301 tcgtcttctc cgtgctcagg cgggtggcga agaaccgcga cgtgttctgg cgcggcgtgg
      361 tcgagcgcgg ccgggccaag ctgcgggtga tgatgatcat catcgccatc ggcatcgggg
      421 tgacagtctc gccgatggat cccggaccgt cgctacagat ccgctcggtg ctggtgttcg
      481 tcttcatcct gacgctgggg tggctggcgt cgggcgtcct cgacatgtgg acggccatgc
      541 acctgcggcg gttcaacatg gccgccgaag acaatctgat cgcgcgcaaa cactatacgc
      601 aggcgcggat attgcagggg gtggccaagg tgctgctggt catcatcacc gtcgggctgg
      661 cgctgatgac gatcgcgggg ttccgccagt ggggcgtcag tctgctggcg tcggcgggtg
      721 tcgtcggcat catcgccggt ctggcgctgc agccggttct gaccaatctt ctggccggta
      781 tccagatcgc cctgacccag ccgatccgca tcgacgacgc cgtcatcgtc gagaacgagt
      841 ggggcaatgt cgaggagatc accgcgacct atgtggtggt caagctgtgg gactggcgcc
      901 ggatggtgct gccgctgtcg tggttcatca ccaagccgtt ccagaactgg acgcgcgaga
      961 acgcccggct gataggcacg gcgatgttct acgtcgacta cgaggcgccg atcgacaagc
     1021 tgagggccgg gttcgaggcc atcgtcaaga actcgaagct ctgggacggc gacgtgcagg
     1081 tgatgcaggt cacggacatc accgagcggg tgatgcaggt gcggtgtctg gcgagcgcgc
     1141 gctcggcgcc ggtggcgttc gatctgcgct gcgaaatccg cgagaagctt atggctttca
     1201 tgcgcgatga atgcccggag gcgctgccga gagatcgcgt ggatctggag cgtatcagtg
     1261 cgggtccaga tcgcccgcca gagccgctcg acccaacgac accgccgcct caagcgtaga
     1321 agcgtcggcg gggcggtcgg gatcgccaca ggccacggcg agcagctcgg cggcgtcccc
     1381 accgggtccg gcgctatagg caggttggtt gtcggggctg gacgggcctt cgacgccgcc
     1441 ctcgcgaacg gatgcgaagt cgagacggtc agcggtcatg gccttgcagt cgaatgacca
     1501 gcgcgaccag ccgccccaat aggcggtgcc gccgaccgcg aagccgggct cgacgacctg
     1561 gaacgaccgc agggtggcga tgtcaccggc gcgctggatg gtggtcagat cggcgaacac
     1621 cgcgaaactg ccggcgcccg tgagcaagaa gcggtgctcg gcgggcgcgg ttgtctgggc
     1681 agttgcaggc agggcgcacg ccatcgcggc gacggcgagc gcgatcctga gcatcaaaat
     1741 cagttcgggc ggaacaggcc ggcggggctg gcggtgaaga tttctacgcc agtctcggtg
     1801 accccgatcg tgtgctcgca ctgggcggac agggacttgt cgcgggtgac ggcggtccag
     1861 ccgtcggaca ggaccttcac gtgcggcttg cccagattga ccatgggctc gatggtgaag
     1921 cacatgccgg gtttcagcac ggcgccctcg cccttgcggc cgtagtgcag gacgttgggg
     1981 ctgtcgtgga aaacgcgacc gatgccgtgg ccgcagaagt cgcgcacgac gctcatgcga
     2041 gcggcttcga cgtgacgctg gatggcgtag ccgatgtcgc cgaaggtgtt gcccggcttg
     2101 acctgttcga ggcccagcat cagcgattca taggtgacgt cgacgagctt ctttgtgcgg
     2161 gcgttgatat tgccgaccga atacatgcgg ctggagtcgc cgtgccagcc gtcgacgatg
     2221 acggtgtggt cgatgttgac gatgtcgcca tccttgagca cccggtcgcc cggaatgccg
     2281 tggcagacga cgtggttgat cgaggtgcag acggtcttgg tatagccccg gtaaccgagg
     2341 caggcgggca ggccgccgcg ctcgatggtg tactcgcgaa tgcggtcgtc gagctcgccc
     2401 gtggtcacgc ccgggaccac gaacggcgtg atcatgtcca gcgcctcggc gaccagccga
     2461 ccggcgatgc gcatgccctc gaaatcatcc tgatcgtaga tgcggatcgc gttcgaccgg
     2521 gtgaaggtct cggtttccag ttcgggagtc tcgtacatcg tgcagctcgg gaggagggcg
     2581 ttcgggcggg aacgtcaggg ggcagatggc gacgatagca cgaaacctca accctgtccg
     2641 ggcggagcga tcaagccgtc gcgacaatcc agcgcgcgat gagaccggca agggcttcgc
     2701 atttggcttc gatttcggcc cggtcggcca accgcaccac gccgcgatcc ggtgaaggcc
     2761 agacggctag tcggtccggg tcttcgaaat gaaagcctgc cgtatctgca accttggcgc
     2821 cccggtgcag gaccacccgg tatccgcctc tcggctctat gccgagggtc acccggtcac
     2881 gcccctcgtg ggcatagttt ggggcgttcc atttgatctc ttcgtgcaag gcgccgcccg
     2941 agttgcggac cacttcgcgc aaggcgtcga tggccgctcg ggtcggctga tcgagcgagt
     3001 cgagccagac ggtgacggtg ggggcgggct tggccatacg ggttcctgac gcggcgatca
     3061 gcgacgacag gatgcgatcg tcatccctcg tatatcatct tgcggctcat cccgccgtcg
     3121 acggtcagca cctgtcctgt gacgaagccc gcgccggcca gatagacgac ggcgtcggcg
     3181 atgtccgggg cttccccgac ccggcccacg ggatgttgct cgtggtcgac gtccctcagg
     3241 tccttgccgt ccgagatcca gcccggagcg atggcgttga cgcgcaccct ggggccgagg
     3301 ctgatcgcca gcgcatgggt cagggcgacg agaccgccct tggacgccgc ataggcctcg
     3361 ctgtccggct cggacatgaa ggcgcgggtc gagaccatgt tgacgacgct ggagccttcg
     3421 cccagcagcg ggaccgccgc gcgggtcatc aggaaggcgc cggtcagatg gctgtcagtg
     3481 accttgcgcc agtcggccag cgacaggtcg gcgatggagc cgctggtggc gttggcgaca
     3541 cctgcgttgt tgaccagcag ctcgagttcg ctccagccga gttccgcgaa ggcggcggcg
     3601 acggaagcct catcggcgat gtcgcacatg atggagcggg cttcggacgg gccgggtttg
     3661 aggtcgaacg acgcgacctg ccagccgttg gctgcgaggg tctggcagat gcccgagccg
     3721 atcaggcctg ctccgccggt gacgatggct ttcttcatgg gagctttaat cggcgagggc
     3781 gtggcgagtt ccgtagcgtc acgggcaccc gttaatggcc gctatcgacc cattgcggac
     3841 gttggctgaa tgccgttaat ccagatcatg atccaactac gagacaaaga gcgtttcccg
     3901 aaccctgttg cgatagctgc gcttgcagag aggttcaacc tgcccaacga cagcaacatg
     3961 caagactggg agtacgtcgt cgctgacctc gcgcggctcg atgagttctt ggcgtacgcg
     4021 gagtcctgtc ctgatgaaga tgaggcattt gtcctcatca agttgatatt ccagtcattt
     4081 gaagacttgg gaggcacagc gctcgaagat ccgcggtggg cccgggtaga taagctgttg
     4141 aataccgatt ttgaacttca tctgtcgacg ctccggtatt gggcttcggc tgatcaaatt
     4201 tcagaagacg gctggcatat cgcgcctctc gtacgttcag ttatcgcctc gcacggtgta
     4261 gaaaaactgt cgccatcctg aaggcgcatg accacttccc acccttaggc gacattggtg
     4321 ataacggcac accggacgaa cgttcgcttt caggccggac gccgagctcg caaatcgacc
     4381 cgttgcggac gttgattcat cgacgccaaa tgatttgatg gaaacggccc catccagccg
     4441 gatcatggat cagcgcatcc gaaaccggat catggaggaa ctgctcggat tggcagaagg
     4501 cgacgaaaac attcgccaga tcggagccgg agaatacttc gaagccttct acgattgggt
     4561 tccgcatcgc ggcgacggca tcagaccgaa cggtgcgctg acacaggatg aagctgcctt
     4621 gatccttaat gtcagcatcg ctttggatga ggcttgggac gccacgccgg gaatggtgtc
     4681 ggctgaagaa ctcatcgcta ctggttggcc tgaaaggatc aagcccctcg cgcagtctgc
     4741 gctggaccag atgctgaagc gaggtagatt cagtgaagac gctgaagagg acgagccgtc
     4801 ttcagccatt ccttggccgt agcgaagtcc gctttccacc catctcagac attggcagtg
     4861 tccgccttca gcccttacgc caccaacttc tcggcctgat gcaggtcgac gctgaccaac
     4921 tggctgacac cccgctccgg catggtcact ccgtacagcc ggtccatgcg gctcatggtc
     4981 accgggttgt gggtgatgac gatgaagcga gtgtcggtgc ggttgcgcat ttcgttgagc
     5041 atgcggcaga agcggtcgac gttggcgtcg tccagaggcg catcgacctc gtcgagcacg
     5101 cagacgggcg cggggttggc gagg
//
LOCUS       DASVTM010000118         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_757475,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000118 DASVTM010000000
VERSION     DASVTM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757475"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /gene="glf"
                     /locus_tag="VF633_03390"
     CDS             <1..1394
                     /gene="glf"
                     /locus_tag="VF633_03390"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX8470134.1"
                     /translation="IRHYGELEAVAVADTPEAFIAACDAALALARDSAGEWLQAADTA
                     LADLSWDQTHIRMSALIERAGLAKRQKVAEPTRRRRAPQPRYDVLVVGAGFAGSVLAE
                     RLASEGGQSVLVVDRRPHVAGNAYDHLDAAGVMIHQYGPHIFHTNSVEVFAYLSRFTR
                     WRPYEHRVLAQVGDNLVPMPINRTTLNILFGLHLQTDEDAAAFLASRAEPVSVVKTSE
                     DVVVTAIGRELYETFFRGYTRKQWGLDPSQLDKSVTSRVPTRTNTDDRYFTDTFQAMP
                     LDGYTAMFKNMLDHPNIEVRTGVEYKDIAATTPHDRLVFTGPIDEFFDYRYGRLPYRS
                     LKFRHETHDRETFQPVANVNYPDEATPYTRITEYKHLTGQQHPSTSITYEYPTADGDP
                     YYPIPRPENQALFKRYEALAAERPDVSFVGRLATYRYYNMDQVVGQALSTWRRLAPQL
                     GHAPEDRVAAQRG"
     gene            complement(1453..2652)
                     /gene="glp"
                     /locus_tag="VF633_03395"
     CDS             complement(1453..2652)
                     /gene="glp"
                     /locus_tag="VF633_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX8470135.1"
                     /translation="MSLPTVATALATMLAEVQPLAGECVPVDRADRRWLSEDVAATRD
                     QPPFDSSAMDGWAVRTVDVAVRASLRIVGESAAGHRSGVPLKAGETVRISTGAPLPEG
                     ADRVVMQEDAERLGDLLAYGPAGLAPTYVRPRGCDFREGETLLRAGERMNPWRLALAA
                     SAGAGILRCGRLPRVAIVATGDELVEPGQAPGPDQIYNSGAPSVGAFVMRHGGEAVRV
                     ATASDSEVAIIDAIGGVDFDILVTIGGASVGDHDLVKPALKSHGAIMHIEGVAVRPGK
                     PVWFAVLADGRPVLGLPGNPASALVCAELFLAPLLARFQGGSAEGRFETAVLATDLPA
                     NGARDHYIRAHAVAGTDGRRRVTPFTDQDSSLVTIMAAANVLIRRPPGAPSATAGTAV
                     TILGPTL"
     gene            complement(2649..>3023)
                     /gene="moaC"
                     /locus_tag="VF633_03400"
     CDS             complement(2649..>3023)
                     /gene="moaC"
                     /locus_tag="VF633_03400"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX8470136.1"
                     /translation="MMLPETLALALSRDGKKGDVRSVAEIAGVMAAKRTSDLIPMCHP
                     LMLSSVKVSVEPMPDGVGLGVNARVRTTGQTGVEMEALTAASVALLTLYDMLKAVDRG
                     MTIEGVRLLNKSGGASGEWARA"
BASE COUNT          555 a         1040 c          909 g          519 t
ORIGIN      
        1 tcatacgtca ttatggtgag ttggaagcag tcgccgtcgc cgatacgccg gaagccttca
       61 tagcggcttg tgatgcggct ctggctcttg cccgcgattc tgccggcgag tggctacaag
      121 ctgccgacac ggcactggcg gatctttcgt gggaccagac acatatccgc atgagcgcac
      181 tcatagagcg cgctggtctg gccaagcgtc agaaggtggc cgagcccacc cgtcgtcgcc
      241 gtgctccgca gcctcgctac gatgtgctcg tcgtcggcgc cggctttgcg gggtccgtcc
      301 ttgccgaacg tctcgcctcg gagggcggcc agagcgtgct ggtggtcgat cggcggccgc
      361 acgtggccgg caacgcctac gatcatctcg acgcagccgg cgtgatgatc caccagtacg
      421 ggccgcacat cttccatacc aactcggtgg aagttttcgc ctacctgtcg cgcttcaccc
      481 gctggcgtcc ctatgagcat cgggtgctgg cccaggtcgg cgacaacctc gtacccatgc
      541 caatcaaccg cacgactctg aacatccttt tcgggctgca cctgcagacc gacgaagacg
      601 cggctgcatt cctggcatcg cgcgccgagc cggtttcggt cgtgaaaacc tccgaggacg
      661 ttgttgtcac agcaatcggc cgcgagctct acgagacctt cttccggggt tatactcgca
      721 agcagtgggg tctggatccc tcgcagctcg acaagtcggt gacctcgcgc gtccctaccc
      781 gcaccaatac agatgatcgc tattttacag acacgttcca ggccatgccg ctggacggtt
      841 acacggccat gttcaagaac atgctggacc atccgaacat cgaggttcgg accggggtcg
      901 agtacaagga catcgccgcg acgacgcctc acgaccgact ggtgttcacg ggccccatcg
      961 acgagttctt cgactatcgg tacggccgcc tgccctatcg ctcgctgaag ttccgccacg
     1021 aaacccatga tcgcgagacg ttccagcccg tcgccaacgt caactatccg gatgaagcga
     1081 cgccctacac gcgcatcacg gaatataagc acctgaccgg ccagcagcat ccgtccacca
     1141 gcatcacata cgaatatccg acggcagacg gcgaccccta ctatccgatc cctcgccccg
     1201 aaaaccaggc gttgttcaag cgctatgagg cattggcggc ggagcggccg gatgtcagtt
     1261 tcgtcggacg gctggcgact taccggtatt acaatatgga ccaggtcgtc gggcaagcgc
     1321 tgtctacctg gcgtcgtctg gcgccgcagc tcggccacgc gccagaagac cgggtggccg
     1381 cccagcgcgg ttagcgtggc agaaagggag aagtcgcaga ggcaaagcaa aaatgcggcg
     1441 ccgctataaa ccctacagcg tcggtccgag aatggtcacg gcggtccccg cagtcgccga
     1501 aggtgcaccc gggggacgtc tgatcaggac gttggcggcc gccatgatgg tgaccagcga
     1561 cgagtcctga tccgtgaagg gcgtaactcg tcggcgaccg tccgtacccg cgaccgcatg
     1621 agcccgaatg taatgatcgc gcgcgccgtt ggcagggagg tctgtcgcca gcaccgctgt
     1681 ctcgaagcgg ccctcggccg atccaccctg aaaccgcgcc aacaagggtg caaggaacag
     1741 ttcggcgcaa acgagcgccg aggcggggtt gccgggaaga cccagcacgg gtcgcccgtc
     1801 tgcgagcacg gcgaaccata ctggtttgcc cggccgcacg gccacgccct caatgtgcat
     1861 gatcgctccg tggcttttga gcgccggctt gacgaggtcg tggtcaccga cagaagcacc
     1921 gccgattgtt accagaatgt cgaaatcaac accgccgatg gcgtctatga ttgccacttc
     1981 gctatcgctc gcggtcgcca cacggaccgc ctcgccgcca tgtcgcataa cgaaggctcc
     2041 aacggagggc gctccggaat tgtagatctg gtctgggccc ggcgcttggc ccggctcgac
     2101 gagctcatcg ccggtggcca cgatcgcaac tctgggcaat cgcccacagc ggaggattcc
     2161 ggcccctgcc gatgccgcca gcgccagccg ccaggggttc attcgttccc ctgccctcag
     2221 aagggtttcg ccctcccgaa aatcgcaccc gcgaggccgg acgtaggtcg gggccaatcc
     2281 ggcgggtccg tacgccagca gatcgcctag tctctcggcg tcctcctgca tgacgacccg
     2341 gtccgcgcct tccggcaagg gagcccctgt agagatgcgc acggtctctc cagctttcag
     2401 gggcacacca gaccgatgtc ccgccgcgct ttcgcctaca attctgaggg aggcccgcac
     2461 cgccacatcc acagttcgca ctgcccagcc gtccatggct gaagagtcga acgggggctg
     2521 gtcacgcgtc gctgcaacgt cttccgacag ccagcggcgg tcagcccggt ccaccggaac
     2581 gcactcgcct gccaaaggct ggacctcggc aagcatcgtc gccagcgccg ttgcgacggt
     2641 gggcaggctc atgctcgggc ccactcaccg gacgccccgc cggacttgtt cagcagccgc
     2701 acgccctcga tcgtcatgcc gcgatcgacg gccttcagca tgtcatagag cgtcagaagg
     2761 gccacgcttg ccgctgttag agcctccatc tcgaccccgg tctggccggt cgtccgcacc
     2821 ctggcgttca cgcccagccc cacaccgtcc ggcatcggct cgaccgaaac cttgacggag
     2881 ctcagcatca gcggatggca catcgggatc aggtcggagg tgcgcttggc ggccatcacg
     2941 ccagcgatct cggctaccga acgcacgtcc cccttcttgc cgtcccggga cagcgcaagc
     3001 gccagggttt ccggcagcat cat
//
LOCUS       DASVTM010000119         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_704657,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000119 DASVTM010000000
VERSION     DASVTM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704657"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF633_03405"
     CDS             <1..322
                     /locus_tag="VF633_03405"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX8470137.1"
                     /translation="LDRTAEGPDHAASLEPGEMAALVRGARRVTEALGDVIKRPVAAE
                     IPNIAVARRGLVAARPIAVGEIFSAENLTARRAGGGLPPGRLWELIGQVATRAYAADE
                     KIEA"
     gene            319..1488
                     /gene="neuC"
                     /locus_tag="VF633_03410"
     CDS             319..1488
                     /gene="neuC"
                     /locus_tag="VF633_03410"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX8470138.1"
                     /translation="MSGAKARIGVFTATRAEYGLLRPLLERLSASDRLEPHLIVSGTH
                     LSGRHGMTVSEIESDGRSIAARIPVPLAADDGVSIAQDMAAVLAGAAAAYATLKLDAI
                     LILGDRTEALAAAAAAVPLGLPIIHLQGGERTEGAVDDNIRHALTKLASFHFAAAEPY
                     RRRILQMGESPDRVVTAGSTAIDNQRTFGRSTRAEVARMLNIDLEPGFLLITFHPETL
                     TGIPAIDQIAAFLGGLAHETRRKLVITLPNADTGADAVREGLASFAAARPGQVYLFES
                     LGARRYAAALTACDAVVGNSSSGVIEAPGAGTPSVNVGDRQAGRLKPSSVIDCANTPE
                     AIAAALGRALDPAFRQAARHQPPPFGDGHAADRIVEVLERTDFRGLARKAFVDRD"
     gene            complement(1485..2177)
                     /locus_tag="VF633_03415"
     CDS             complement(1485..2177)
                     /locus_tag="VF633_03415"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEX8470139.1"
                     /translation="MIDGKRVLGLVPARAGSQGLPGKNMRPLAGRPMIGWTIAAGQAS
                     TTIDRLVVTTDDDAIIALAAEMGVETVRRPAELAGAKASVIDAIDHALTQLGGEWDYV
                     VLLQPTSPLRTAVDVDGAVSLCHDCAAPSVIGVSPMPKPPGFYGSVSPDGLWVRNGPS
                     PDEAVVINGAIYVGRPDILRRDRSFQSEGTLTWPLPPERGWDVDTLFDFVVCEALWPR
                     VQSGEALGGVRR"
     gene            2301..>3204
                     /locus_tag="VF633_03420"
     CDS             2301..>3204
                     /locus_tag="VF633_03420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX8470140.1"
                     /translation="MIDGSEEVFNAYRGKRVLVTGAGGFIGSRLTERLVELGASVRVL
                     VRYTSDGDAGWLDRSGVRGQLGVRRGDLADRDSVYDAVKDREIVFHLGALIAIPYSYL
                     APESYVRTNMMGTLNVLQAVRELGVSRLVHTSTSEVYGSAQIIPMTEAHPLVGQSPYS
                     ATKIAADKLAESYHRSFETPVVTLRPFNTFGPRQSARAVIPSITMQALAGRPVKLGDT
                     RPTRDFVFVDDTADAFLRAGIVHGIEGLTVHTGGGREVRIGDLPAMIAKAGGIKVEVV
                     EDAQRIRPAASEVERLVADASLARE"
BASE COUNT          550 a         1011 c         1088 g          555 t
ORIGIN      
        1 gctcgaccgc acggcggagg ggccagatca tgcggcttcg ctggagccgg gcgagatggc
       61 tgcactggtt cggggcgcga gacgtgtgac cgaggccctc ggcgatgtga tcaagcgacc
      121 cgtcgcggcg gagatcccga acatcgcggt ggcgcgcagg ggcctcgtcg ctgcgcgccc
      181 aatcgcggtg ggagaaatct tcagcgctga aaatctgacg gcgcgacgcg ccggaggcgg
      241 gcttcctcca ggacgacttt gggagctgat cggccaggtc gctacccgcg cctatgcagc
      301 cgacgagaag atcgaagcgt gagcggggcc aaggccagga tcggtgtgtt tacagctacg
      361 cgcgctgaat atggtctgct gcgacccttg ctggagcgac tgtcagcatc cgatcgcctc
      421 gaaccgcacc tgatcgtcag cgggacccat ctttccgggc gccacggcat gaccgtctcg
      481 gagattgaga gtgacgggcg atcaatcgca gcccgcattc cggttccgct agccgcagac
      541 gacggcgtgt cgatcgctca ggatatggcg gccgtgctgg cgggggccgc cgcggcctat
      601 gcgacattga agctcgacgc gattctgatc ctcggtgatc ggacggaggc gttggcggct
      661 gcggctgcgg cagtgcccct gggtcttccc atcatccacc ttcaaggcgg tgagcgcacc
      721 gaaggcgccg ttgacgacaa cattcgtcat gccctgacca agctcgcctc attccatttc
      781 gctgcggccg aaccctaccg gcggcggata ctgcaaatgg gagaaagccc cgaccgggtg
      841 gtgactgccg gatccacggc gatcgacaac caacggacgt ttggacgcag tacccgggct
      901 gaagtcgccc gcatgctgaa catcgatctc gaacccggct ttctgctgat caccttccat
      961 ccagagactt tgaccggcat tcctgcgatc gatcagattg cagctttcct cggcggcctg
     1021 gcgcacgaga ctcggcgaaa gctggtgatc accctgccga acgcggacac cggtgcagat
     1081 gccgtgcgtg agggtctggc ttcgttcgca gcagcacggc cgggtcaggt ctatctgttc
     1141 gagtccctcg gcgcgcggcg gtacgctgct gcgctgacgg cctgcgatgc tgtggttggc
     1201 aactcctcca gcggcgtgat cgaggcgccg ggggcaggga cgccttcggt caatgtcggt
     1261 gatcggcagg ccggtcggct aaagccatcc agcgtcatcg actgcgccaa cacgcccgag
     1321 gccatagccg ccgccctggg acgggcgctc gacccggcct ttcgccaagc ggcgcgccat
     1381 cagccgccgc cattcggtga tggccacgcc gcggaccgga tcgtagaagt gcttgagcgg
     1441 accgacttca gggggttggc gcgcaaggct tttgtggatc gggactagcg cctcactcca
     1501 ccgagtgctt cacccgattg gacgcgcggc cagagggctt cgcaaaccac gaagtcgaac
     1561 agggtgtcga cgtcccagcc gcgttcgggg ggcaggggcc aagtcaatgt gccttccgac
     1621 tggaacgacc ggtcccggcg aaggatatca ggccgcccga catagattgc gccgttgatg
     1681 acgaccgctt catccgggct ggggccgttt cggacccaaa gcccgtccgg gctgaccgag
     1741 ccatagaacc cgggcggctt gggcatcggg gagaccccga tcaccgacgg cgctgcacag
     1801 tcgtgacaga gagacaccgc cccgtcgaca tcgacggccg tgcgcagtgg tgaggtcggc
     1861 tgcagcaaga ccacgtagtc ccattcgccg ccaagttggg tcagggcatg gtcgatggcg
     1921 tcgatcacgg aggccttggc gccggccagc tcagcagggc ggcgcactgt ctcgacaccc
     1981 atttcggcgg cgagagcgat gatggcgtcg tcgtcggtgg tcacaaccaa acggtcgatg
     2041 gtggttgatg cctgacctgc ggctatggtc cagccgatca ttggtcgtcc agcgagagga
     2101 cgcatgttct tgcccggcag gccttgcgag cctgcacggg cgggaacgag tcccagcacc
     2161 cgtttgccgt cgatcatggg cgttctcctc gcaacgtcac cagaatgaag gcgcgggcct
     2221 tacgcccggt taaccacgtc tggaggatca tggaccttat agcgagacgt caagtgacgt
     2281 gaccgcacgg agcagacgac gtgatcgacg ggtcggaaga ggtcttcaac gcctatcgag
     2341 gcaagcgcgt gctggtcacc ggtgcgggcg gcttcatcgg cagccgtctg actgagcggc
     2401 ttgtcgagct cggcgcgagc gtgcgggtgc tggttcgcta cacctccgac ggggacgcgg
     2461 gctggctgga ccggtcaggt gttcgaggcc aactcggtgt ccgacgtggg gatctggccg
     2521 atcgcgacag cgtgtatgat gcggtcaagg atcgggagat cgtctttcac ctcggggcgc
     2581 tgatcgcgat cccctattcg tacctggccc cggaaagcta cgtccgcaca aatatgatgg
     2641 gcacgctcaa cgtgcttcag gcagtgcgcg agctgggtgt gtcgcgtctg gtccatacct
     2701 cgaccagcga ggtctatggc tcggcccaga tcatcccgat gaccgaggcg cacccgctcg
     2761 tcggccagtc gccctattcc gccaccaaga tcgccgccga caagctggcc gagagctatc
     2821 atcgctcgtt cgaaacgccc gtggtgacgc tgaggccgtt caacactttt ggtccccgcc
     2881 agagcgcccg ggcagtgatc ccgtcgatca ccatgcaggc tctggctggt cgtcctgtga
     2941 agctggggga cacccgtccg acgcgagact tcgtcttcgt ggacgacacc gccgacgcct
     3001 tcctgcgggc gggcatcgtg cacggcatcg aagggctgac tgtccacacg ggtggcggtc
     3061 gcgaggtccg gatcggagac ctgccggcga tgatcgccaa ggcgggcggg atcaaggtcg
     3121 aggtggtcga ggatgcgcag cgcattcgtc ctgccgccag cgaggtcgag cggttggtcg
     3181 ccgacgcgtc ccttgccaga gaaa
//
LOCUS       DASVTM010000120         4994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_116914,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000120 DASVTM010000000
VERSION     DASVTM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116914"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..386
                     /locus_tag="VF633_03425"
     CDS             252..386
                     /locus_tag="VF633_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470141.1"
                     /translation="MRRALHIGLFIGTLIGMCACASMDGANTGQSGMYGGAEVGRTGR
                     "
     gene            542..1876
                     /locus_tag="VF633_03430"
     CDS             542..1876
                     /locus_tag="VF633_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX8470142.1"
                     /translation="MLLIAIVVVLLLVVLNGLFAMTELAVVSSRRSKLQSRAEKGDRG
                     ARAALKLAEDPTHFLSAVQVGITLIGILAGAYGQAAIAGELDKILEAAFPALIAYTQI
                     FSTAIVVVFITYVSVIVGELVPKRLALIFPETIAAKMAGPVSGLAIVLKPFVVVLTAS
                     TSGILKLMGVKDRDGSDVTQEEVETMIAEGTSAGLIEPEEQVMIEEILRLGDRPVRVA
                     MTPRTDVYWVALDDAPEVLREEIRVCPYSRIVVAKDGDLDHPMGVVHKKDLLDALLTT
                     GEMNVGEHIAEPAFIPQSTSVLKALEILKGTHVHMAFIVDEYGAFEGVVTATDILEMI
                     AGDFNESHDEDRSHVTQRADGSWLVDGRTDLDELCDLLGEDFGENESFHTVAGLVLHQ
                     LSRVPDEGEILQLGRFEVEVVDMDDRRIDKLIFKQVVNAEDEKKALAEHYEG"
     gene            1994..2069
                     /locus_tag="VF633_03435"
     tRNA            1994..2069
                     /locus_tag="VF633_03435"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2027..2029,aa:Trp,seq:cca)
     gene            2139..2444
                     /gene="secE"
                     /locus_tag="VF633_03440"
     CDS             2139..2444
                     /gene="secE"
                     /locus_tag="VF633_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX8470143.1"
                     /translation="MAKSPGKPTRPGFRPGKAPVAAGAPATVTIDSAAPKKKTSIPQF
                     FSQVRAEARKIVWPSRKETWITSVMVFIMVLIATLFFWIVDSGLTFLSQIILAIGQR"
     gene            2457..3050
                     /gene="nusG"
                     /locus_tag="VF633_03445"
     CDS             2457..3050
                     /gene="nusG"
                     /locus_tag="VF633_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX8470144.1"
                     /translation="MTDAAPISTDTAAKPVNPRHKWYIIHAYSNFEKKVAQHIRDQAK
                     QRGLEDNFSEILVPTEDVIEIRRGRKVNSERKFFPGYVLARMEMTDDAYHLVKDTPKV
                     TGFLGAAGGTKPLPVSEREVQAIIGAVEEGVERPKPTIRFEIGETVKVIDGPFASFEG
                     QVESVDEDAARLRVAVSIFGRPTPVDLEYNQVEKNAA"
     gene            3403..3834
                     /gene="rplK"
                     /locus_tag="VF633_03450"
     CDS             3403..3834
                     /gene="rplK"
                     /locus_tag="VF633_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX8470145.1"
                     /translation="MAKKILGYIKLQVKAGSATPSPPIGPALGQRGVNIMGFVKEFNA
                     RTEKEVKGTPLPTVITVYQDKSFTFITKTPPATHYIKQAAGIESASKLPGREVAGKIT
                     RTQLREIAEKKMKDLNANDLDAATKIIEGSARAMGLTVVEA"
     gene            3838..4524
                     /gene="rplA"
                     /locus_tag="VF633_03455"
     CDS             3838..4524
                     /gene="rplA"
                     /locus_tag="VF633_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX8470146.1"
                     /translation="MAKQTKAHKARTGDTLTPISFTAAIAAVKENAKAKFDESIEIAV
                     NLGVDPRHADQQVRGVVNLPSGTGRDVRVAVFAKDAKADEARAAGAEYVGAEDLYEKI
                     NGGFMDFDRVIASPDMMALVGRLGKVLGPRGLMPNPKVGTVTPNVAQAVKDAKGGAVE
                     FRVEKAGIAHGGIGKVSFTQEALEANVKAYVEALNRAKPAGAKGTYVKRITLSSTMGP
                     GFKIDPASLA"
     gene            4655..>4994
                     /locus_tag="VF633_03460"
     CDS             4655..>4994
                     /locus_tag="VF633_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470147.1"
                     /translation="MAVVLAALTVGPLSAHAEGTSANVPASTAVRDEAAVKLELARRF
                     IAVMQTDQLGAMIGQMTTSMTPTKDGMSQDESDAMRRAIQRAAGEMMPRMFDAMAPIY
                     ADIFTLEELTG"
BASE COUNT          980 a         1556 c         1546 g          912 t
ORIGIN      
        1 gccggcgagg ggttccagat ctacgattcg accttcgacc tgcccgccat tgaagtggac
       61 ggtgtcagcg ggttcctgac tgctgacggg ggcaaggtcg agaacctttt cggcggcgat
      121 acatgcatgg ccggctgaga cggtagctga cgaagacgga aggctgctga gcgtgtcccg
      181 ccgtgagacc ttgcgcggct catgacttgc aggcatttcg cttatcggac gttaacgttt
      241 tggtaagcat catgcggcgc gcacttcaca tcggcctctt catcggcacc ctcatcggaa
      301 tgtgcgcctg cgcctccatg gacggcgcca atacgggaca gtccgggatg tacgggggcg
      361 ccgaggtggg gcgcaccggc cgctgaccgg tctgcgctga cccctgaaac agcaatttcg
      421 cgggcgggca gggctgtgtt agtcgactcc agcaaggaaa acgtggcgga aacggtcgac
      481 ttcggctatc cacgaacagc ggacgccggc tgtcgcgccg cgctggttct ctccccccta
      541 gatgttactc atcgccattg tcgtcgttct gctcctcgtg gtgctcaacg ggctattcgc
      601 catgaccgaa ctcgcggtcg tatcctcgcg ccggtccaag cttcaaagcc gggcagagaa
      661 gggggaccgg ggcgcgcggg cggcattgaa gctggccgag gatcccaccc atttcctctc
      721 agcggtccag gtgggaatca ccttgatcgg gattctggcg ggtgcctacg gtcaggccgc
      781 catcgccggt gagctcgaca agatcctcga agccgccttt ccggccctga tcgcctatac
      841 gcagattttt tccacagcga tcgtcgtcgt gttcatcacc tatgtctcgg ttatcgtcgg
      901 cgaactggtg cccaagcgac tggcgctgat ctttcccgag accatcgcag ccaagatggc
      961 tgggccggtc tccggcttgg cgatcgtgct gaagcctttt gtcgtagtgc tgacggcctc
     1021 gacctccggc atcctcaagc tgatgggcgt caaggaccgg gacggttcgg acgtgaccca
     1081 ggaggaggtc gagaccatga tcgccgaggg cacctcggcg ggcctcatcg agcctgaaga
     1141 gcaggtgatg atcgaggaaa tcctgcgtct cggcgaccgt cctgtgcgcg tggcgatgac
     1201 gccgcgtacc gacgtctact gggtcgcgct ggatgatgct ccggaagtgc tgcgcgaaga
     1261 gattcgggtt tgtccctatt cgcgcatcgt ggtggccaag gacggcgatc tggatcaccc
     1321 catgggcgtg gtccacaaga aggatctgct ggacgccctg ctgaccacgg gcgaaatgaa
     1381 cgtgggcgag catatcgccg agccggcctt catcccgcag tcgacctcgg tgctgaaggc
     1441 gctggagata ctgaagggca cccacgtcca tatggccttc atcgtcgatg agtacggcgc
     1501 attcgaaggc gtggtcaccg cgaccgacat cctcgagatg atcgcaggcg acttcaacga
     1561 aagccatgac gaggaccgtt cgcacgtcac ccagcgcgcc gatggcagct ggctggtgga
     1621 cggccggacg gatttggacg aactctgcga ccttctgggc gaggacttcg gcgagaacga
     1681 aagcttccat accgtcgccg gtctggtgct gcaccagttg tcacgcgtcc ccgacgaagg
     1741 cgaaattctg caactcggcc gattcgaggt cgaggtcgtc gatatggacg atcgtcgcat
     1801 cgacaagctg atcttcaagc aggtcgtgaa cgcggaggac gaaaagaagg ccttggcgga
     1861 gcactacgag ggttgatcct gcccgattaa ggcggattgt ccttgaaaac gcctgaccgg
     1921 tcgggcatac gccgcggtct ggcgaagtac accgcgccga aggggaaacc tgacggagcg
     1981 ttgtgtttta tctaggagct tagctcagct ggtagagcac cggtctccaa aaccgggggt
     2041 cgtgggttcg aatcccccag ctcctgccat ttccccctgt cgtcgccgga tggacttccc
     2101 atctggtgcg acaggttcgt ttgtttgaag agacattgat ggccaagtcc cccggtaagc
     2161 ccacacgacc cggcttccgt cccggcaagg cgccggttgc tgcgggcgcg cctgcgaccg
     2221 tgacgatcga ttcggcggct ccgaagaaga agacttcgat cccgcagttc ttcagccagg
     2281 ttcgcgccga agcccgcaag atcgtctggc cgagccgcaa ggagacctgg atcacctcgg
     2341 tgatggtctt catcatggtt ctgatcgcaa cgctgttctt ctggatcgtg gactccgggc
     2401 tgaccttcct gtcccagatc atcctcgcca ttggccaaag ataaggagtc gcgcacatga
     2461 ctgatgcagc gcccatttcc accgacacgg ccgccaagcc cgtcaaccca cgccacaagt
     2521 ggtatatcat ccacgcctat tcgaacttcg aaaagaaggt cgcgcagcac atccgcgacc
     2581 aggccaagca acgcggcctg gaagacaatt tctccgaaat cctggttccg accgaggacg
     2641 tcatcgagat ccgtcgcggc cgcaaggtga actcggaacg caagttcttc cccggctacg
     2701 ttctggcccg gatggaaatg accgacgacg cctatcacct cgtcaaggac acgcccaagg
     2761 tcacgggctt cctcggcgct gcaggcggca ccaagccgct gccggtctcc gagcgtgaag
     2821 ttcaggccat catcggcgca gtggaagagg gcgtcgaacg tccgaagccc accatccgct
     2881 tcgagatcgg cgagaccgtc aaggtcatcg acggtccctt cgccagcttc gaaggtcagg
     2941 tcgaaagcgt cgacgaagat gcagcccgcc tgcgtgtcgc cgtgtcgatc ttcggccgtc
     3001 cgaccccggt ggacctcgag tacaatcagg tcgagaagaa cgcggcttaa gaagattcac
     3061 ttctccatcg ccgaagggcg atggggagga cagatcgcgc aagcgatcag gagggggcga
     3121 ccgggtgata ccggtgggcc tcctttcttg tttctggtgt tttgaccgtc gggcacggtg
     3181 tcgccctctc cgggtcgtcg ctgcgcgcca accgtcctct ccattcgcct tcgcgaatgg
     3241 agaggtgaag cgtttgcctc tctgcccacg ctctgctaca cgcgcgcctc gctccgcagg
     3301 ggggcggcga ggggcgacct tcgtaaatcc gtgggaggca gccatgccag accacggctc
     3361 accggccccg attagcttcg gggtcactca caggagagac caatggccaa gaagatcctc
     3421 ggctatatca agctgcaggt taaggctggc tccgccacgc cttctccccc gatcggcccg
     3481 gcactgggcc aacgtggcgt gaacatcatg ggcttcgtca aggagttcaa cgcacgcacc
     3541 gagaaggaag ttaagggcac gcccctgccg accgtgatca cggtctatca ggacaagagc
     3601 ttcaccttca tcaccaagac cccgcccgcg acccactaca tcaagcaggc cgccggcatc
     3661 gagtcggctt ccaagctccc gggccgtgaa gtcgccggca agatcacgcg cacgcaactg
     3721 cgcgagatcg ccgaaaagaa gatgaaggat ctgaacgcca acgacctcga cgcggcgacg
     3781 aagatcatcg aaggctcggc tcgcgccatg ggcctgacag tggtggaggc ataaacgatg
     3841 gctaagcaaa ccaaggctca caaggcccgc accggcgaca ccctgacgcc gatctcgttc
     3901 acggccgcta tcgccgccgt caaggaaaac gccaaggcca agttcgacga atccatcgag
     3961 atcgccgtca acctgggcgt cgatccccgt cacgccgacc aacaggtccg tggcgtcgtc
     4021 aacctgccgt cgggcacggg ccgtgacgtt cgcgtcgccg tcttcgccaa ggacgccaag
     4081 gctgacgaag cccgcgcagc cggcgctgaa tatgtcggcg ccgaagatct gtacgaaaag
     4141 atcaacggcg gcttcatgga cttcgaccgc gtcatcgcgt cgcctgacat gatggctctc
     4201 gtcggccgtc tgggtaaggt tctgggtcct cgcggcctga tgcctaaccc caaggtcggc
     4261 accgtcacgc cgaacgtcgc gcaagcggtc aaggacgcca agggcggcgc ggtcgagttc
     4321 cgcgtcgaaa aagccggtat cgctcatggc ggcatcggca aggtttcctt cacccaggaa
     4381 gccctcgaag ccaacgtgaa ggcttacgtc gaagctctga accgcgccaa gccggccggc
     4441 gccaagggca cctacgtcaa gcgcatcacc ctgtcctcga cgatgggccc aggcttcaag
     4501 atcgacccgg cctcgctggc ttaagtcgac cttcaaagtc cgaacgatta cggcggcgga
     4561 gagcaatctc cgccgccgtt tttgttttac tcgtcaggcg caatgcggga atgtttctct
     4621 tagaaaacgg gggcgagcat gaaacgactg atctgtggcg gtggttctgg cggctttgac
     4681 ggtggggccg ctgtcggccc acgctgaggg cacatcagcc aatgtgcctg catcgacagc
     4741 tgttcgagac gaagccgcgg tgaaactgga actggcgcgt cgtttcatcg ccgtcatgca
     4801 gaccgaccag ctgggcgcga tgatcgggca gatgaccacg tccatgacgc cgacaaagga
     4861 tggtatgtcc caagatgaga gcgacgcgat gcgtcgtgcg atccaaaggg ccgccgggga
     4921 gatgatgcct cgtatgttcg acgcgatggc gccgatctac gcggacatct tcaccctcga
     4981 ggagctaacc ggtc
//
LOCUS       DASVTM010000121         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_543118,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000121 DASVTM010000000
VERSION     DASVTM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543118"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1101)
                     /locus_tag="VF633_03465"
     CDS             complement(193..1101)
                     /locus_tag="VF633_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8470148.1"
                     /translation="MAIRFDGKVAIVTGAGGGLGREHALALAARGAKVVVNDLGAARD
                     GSGGSSAAAEAVVAEIVAAGGQAMANGASVTDAESVQAMVDAAVAKWGAVDILVNNAG
                     ILRDKSFAKMTLEDFRFVVDVHLMGAVNCTKAVWDGMRERNYGRIVMTTSSSGLYGNF
                     GQSNYGAAKMALVGLMQTLSIEGAKNNIKVNCLAPTAHTRMTEDLGAALPLDALGPEL
                     VSPGLLYLVSEDAPSRVILAAGAGGFERAYVTITQGDYAAGPDALEQIAERFDEISDR
                     TGEIVPEMGGAQGMIELTKAQKFHGG"
     gene            1334..2488
                     /locus_tag="VF633_03470"
     CDS             1334..2488
                     /locus_tag="VF633_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8470149.1"
                     /translation="MKLKLLASVAVAGLFAAGAASAEPDGWYGAVDAGYHTMEDGINA
                     ESQATGNNFDFEVNDGWAAFARLGYRFNPNWRVELEGGYRSGDVGKVRAVTGNNGVCN
                     ITPATGPCYSPDGDIESTTLMANVIYDFGFEYWGVRPFVGLGAGVNHVNTDVVGRLRN
                     NRAVGFAADDSSTKFAAQAIAGLAWAVSDRANIDLTYRYLTGDAEFNTFTSVPAYNQG
                     VMEGDYDNSHTVTLGLRYAFGAEPVAAPPPPPYVAPPAPPVAPPPPPPAPVAQKPAPR
                     QFVVYFDWDRSDLTAEARSVITAAANYAKSGQPTRLLVVGHADTSGSAAYNIGLSNRR
                     ARTTADALVAQGVAGGVVSLDGKGETQLARATADGVREPLNRRATIDINF"
BASE COUNT          446 a          926 c          758 g          458 t
ORIGIN      
        1 cactggttga gcgcggtttc ctgggttcgc ccccagcttc tgtcggctgg ttcagcggtc
       61 gatgaatagt ctaggcgctt gccgtcacgt caacgcaacc ctctcccggc gggcgagggc
      121 ttgagcctcc gggagcgcag cgatcggcaa ggcgaaaggg tgaggggtgc agtccgatcc
      181 cactcccccg cctcatcccc cgtggaactt ctgcgccttg gtcagttcga tcatgccctg
      241 ggcgccgccc atctccggaa cgatctctcc ggtgcggtcc gaaatctcat cgaagcgctc
      301 ggcgatctgt tccagcgcat ccgggcctgc cgcataatcg ccttgggtga tcgtcacata
      361 ggcccgctcg aaaccaccag cgcccgctgc cagaatgacc cggctcggcg cgtcttcgct
      421 gaccagatac agcaggcccg gcgacaccag ttcaggcccc agggcatcga gcggcaatgc
      481 ggcgcccagg tcctcggtca tgcgggtatg ggcggtcggc gcgaggcagt tgaccttgat
      541 gttgttcttc gcaccctcga tcgacagggt ctgcatcaaa ccgaccaagg ccatcttggc
      601 ggccccgtag ttcgactggc cgaagttgcc gtacaggccg gacgacgagg tggtcatgac
      661 gatccgacca taattgcgct cgcgcatgcc gtcccaaacg gccttggtgc agttgaccgc
      721 gcccatcagg tgaacgtcga cgacgaaacg gaagtcctcc agcgtcatct tggcgaacga
      781 cttgtctcta aggatgccag cgttgttcac cagaatgtcg actgcgcccc atttggcgac
      841 tgcggcatcg accatggcct gaaccgattc ggcgtcggtg actgaagctc cgttagccat
      901 cgcctggccg cccgccgcga cgatctcggc gacgaccgct tccgctgcag ccgaggatcc
      961 gccggagccg tctcgcgctg cgccgaggtc gttgaccacc accttcgcgc ctcgcgccgc
     1021 cagcgccaga gcgtgctcgc gccccaggcc gcccccggct cccgtcacga tcgccacctt
     1081 gccgtcgaac cggatcgcca tctgtcgttc tcccaatatt ttctcgatct ctacgccggc
     1141 gccgcgacgt aagccaagag actcaccagc ggctgcgggc ggtcccgact ggacgatgtg
     1201 ttgaaatggc gccacagtgg ccacaatctc gaacgatatg aggaaccgtc accgagcggc
     1261 gccgttgggg acttcgagca gccacgtgag agcgtcggct atcggcaaaa caattcgaat
     1321 gaggtctaca aacatgaagc tgaaacttct cgccagtgtc gcagtagcag ggctgttcgc
     1381 ggccggcgcc gcttcggctg agcccgatgg ctggtacggc gctgttgatg ccggctatca
     1441 caccatggaa gacggcatca acgccgagtc ccaggccacc ggcaacaact tcgactttga
     1501 agtgaacgat ggctgggcgg cgttcgcccg tctcggctac cggttcaatc cgaactggcg
     1561 cgtcgaactt gaaggcggct accgctcggg cgacgtcggc aaggttcgcg ccgtcaccgg
     1621 caacaacggc gtttgcaaca tcacgcctgc caccggtcct tgctattcgc ccgacggcga
     1681 catcgaatcc accaccctga tggccaacgt catttatgac ttcggcttcg agtactgggg
     1741 cgttcgtccc ttcgtcggcc tcggcgccgg tgtgaaccac gtcaacaccg acgtcgtcgg
     1801 tcgcctgcgc aacaaccgcg ccgtcggctt cgccgccgat gacagctcga ccaagttcgc
     1861 cgcgcaagcc atcgccggcc tggcatgggc cgtttcggac cgcgccaaca tcgacctgac
     1921 gtaccgctac ctgaccggcg acgccgagtt caacacgttc acctcggttc ccgcctacaa
     1981 tcaaggcgtg atggaaggcg actacgacaa cagccacacc gtcaccctgg gcctgcgtta
     2041 cgccttcggc gccgagccgg ttgctgcgcc tcctcctccg ccatacgtcg cgccgccggc
     2101 tccgccggtt gctcctcccc cgccgccgcc cgctccggtc gcccagaaac ctgcccctcg
     2161 ccagttcgtc gtttacttcg actgggatcg ttcggacctg acggctgaag cacgctcggt
     2221 catcacggcc gccgccaact acgcaaagtc gggccaaccg acccgcctgc tggtcgtcgg
     2281 tcacgccgac acctccggct cggccgccta caacatcggc ctgtcgaacc gtcgcgcccg
     2341 caccacggct gacgccctgg tcgcccaagg cgtcgccggc ggcgttgtct cgctggacgg
     2401 caagggtgaa acccaactgg cccgcgccac cgccgacggc gttcgcgagc ctctgaaccg
     2461 tcgcgccacc atcgacatca acttctagtc caaaggggtc cgcatcactg cggaccccaa
     2521 cggtctcgaa cgacgtcgaa gacttcagac cgccggtccc gcgaaagcag gaccggcggt
     2581 tttgcttt
//
LOCUS       DASVTM010000122         5446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_225244,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000122 DASVTM010000000
VERSION     DASVTM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225244"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="VF633_03475"
     CDS             complement(<1..1659)
                     /locus_tag="VF633_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8470150.1"
                     /translation="MTIKTRALCSASILATLLAAPAFAQTTPPAAPQTTDDAAVVDEV
                     VVTGFRRSLQSAQAIKRNSDSIVDAIVAEDIGKLPDVTASESLARVTGVQVTRSAGEA
                     AQVAVRGLLDVSTTYNGREIFTAQDRLVAIQDFGAGTVAALEVYKSGTANLVEPGIGG
                     LINVRSRKPFDFAGREISGSVNAVYWEQAEEPSWNGNLLLSDRWDTGAGEMGLLVNVA
                     YSSVDYLDSTREQALFIAASSPGESPLPSFRFPDSVQQFQGRGTRSRPTANVAFQWRP
                     TEQLEVYIDALYQGYRGRDGDIWNYNPLWGGATFTDVVLRPGSNDAMSLTAQQGPGRV
                     SDGYKTSLDRDTDTWQIGGGLVWTGERLKVSADLATTDSTVTDEKVNLDYAYTGPVTL
                     RVNFDGSGEHGGPSFDLVNYDASNPANVLYRGLFEERARNEGQDVQFRTDFDYEAGWG
                     PITSIQGGLRATDRDARRQYANRYAPGLGLGLNMSQLGLELEVVRGFAFDDGHSFGGI
                     VLPTRDSIMDNVAGLRTIAGQPAGNVPFPTRPELDANEKTFAGYL"
     gene            complement(1734..2939)
                     /locus_tag="VF633_03480"
     CDS             complement(1734..2939)
                     /locus_tag="VF633_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8470151.1"
                     /translation="MRHSLIKAVLAAGFVLCAPMTASAQDAPSRRAIAAAVAGAPDRW
                     TPQQANDWYARQPWLVGANFIPSTAINQLEMFQAETFDPVTIDRELGWAHEKFGMNVM
                     RVYLHDLLWSQDAEGFKSRLDQFLAIAARHDTRIIFVLFDSCWDPDPVLGAQHRPIPG
                     VHNSGWVQSPGREDLIDRANDPRFRAYVEGVVGAFANDPRVLAWDIWNEPDNAGGGSY
                     VDEQLAQESDRIAELLPKAFAWARSRKPVQPLTSGVWVGPDWSPAATTLNAIQKAQLA
                     HSDIITFHNYEWPESFENRIAQLRPYGRPLIATEWLARGNGSTPDTILPIARRENVGM
                     VNWGLVDGDIQTRLPWDSWERPYTLQEPPIWFHDLMHTDGKSYRDREAELFRSIAAGT
                     ANTTTTTAH"
     gene            complement(2957..4057)
                     /locus_tag="VF633_03485"
     CDS             complement(2957..4057)
                     /locus_tag="VF633_03485"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX8470152.1"
                     /translation="MSATPVFAGEAVRTEFGKMADGRAVEAVTLTNDAGVVVRIISLG
                     AAIQSVVVPDRDGVMADVALGYPTIAGYLAKPEFFGATVGRVANRIAGGRFTLDGVAY
                     QVSKNDGENTLHGGAKGFDKVLWEVLDVKSGPDASVTLRYVSPDGDQGYPGTLTATAT
                     YSLNDNGELSVDYGATTDKATVVSMSNHGYWNMAGEGAAAGAMGNVLTIPAAEYTPTD
                     AAAIPTGEFRSVEGTVFDFRTPTAVGARVRDASDEQIVFGRGYDHNWVISRERSAQPR
                     VIATLEDPVSGRTMEVVSDQPGVQFYSGNFFNGTVIGKSGHLYRMGDAIVLEPQAFPD
                     TPNRPEFGSVRLDPGQHYRNHMVFRFSARPRS"
     gene            4200..5192
                     /locus_tag="VF633_03490"
     CDS             4200..5192
                     /locus_tag="VF633_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8470153.1"
                     /translation="MPLFRHGLFLAAAMFVMGFSDARPGPQAPVLDREFADPFVLPVD
                     GGLVAYATNVRERGRGMNVQLSSSIDGRTWSPPRDAMPTRPPWALQRSPDVWAPEVMR
                     IGERYVLFFSARHATRRRPDGLTLCVGAATSDSPEGPFVPQAEPLTCGGPLGVIDASP
                     FRDGDDLWLYVKSDGNCCRIPITIVAQRLSSDGLALQGQPTTIAGLSNDAPWEGRVIE
                     AQQMVLHDGRYIMFYAANDYGGRDYATGYAVCEGPAGPCRDAPENPILKSTSGLQGLV
                     GPGHQSVFQWKDRTWIAFHGWRRPLTDGRWGYRSLYVSPLDWVNGSPVVSSPTP"
     gene            complement(5226..>5446)
                     /locus_tag="VF633_03495"
     CDS             complement(5226..>5446)
                     /locus_tag="VF633_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX8470154.1"
                     /translation="DDGRKLIDKAGAGPIWSRNYDVVTGQPIFGDKDASIHDDVNGIS
                     VGRRNGYSWWIASPQAALDAYPGWAAAN"
BASE COUNT          914 a         1750 c         1792 g          990 t
ORIGIN      
        1 cagatagcca gcgaacgtct tctcgttggc gtcgagttcc ggacgggtcg ggaaaggaac
       61 gttgcctgcc ggctggcccg cgatcgtgcg cagcccggcg acattgtcca tgatgctgtc
      121 acgcgtcggc aggacaattc cgccgaacga atggccgtcg tcgaacgcga agcctctcac
      181 cacctcgagt tcgaggccga gttgcgacat gttgaggccc agcccgagac cgggcgcata
      241 tcggtttgcg tactggcgcc tggcgtcccg atcggtcgcc cgcagtccgc cctgaatgga
      301 ggtgatcgga ccccagccgg cctcatagtc gaagtcggtg cggaactgca cgtcctgacc
      361 ctcgttcctc gctcgctcct cgaaaaggcc gcggtagagc acattggccg gattgctcgc
      421 gtcgtagttc accagatcga acgacgggcc gccatgttcg ccgctgccgt cgaagttgac
      481 ccgcagggtg accgggccgg tgtaggcgta gtccaggttg accttttcgt cggtgaccgt
      541 gctgtcggta gtggcaaggt cggccgaaac tttcagccgt tcgcctgtcc agaccaggcc
      601 gccgccgatc tgccaggtgt ccgtgtcgcg gtcgagggag gtcttgtagc cgtcggaaac
      661 gcgacccgga ccctgctgcg cggtcaggct catcgcgtcg ttgctgccag ggcgcagcac
      721 cacgtcggtg aacgtcgcgc cgccccacag cgggttgtag ttccagatgt cgccgtcgcg
      781 cccacggtag ccttggtaga gggcgtcgat gtagacttcg agctgttcgg tgggccgcca
      841 ctgaaaagcg acgttggccg tcggcctgga gcgggtgccc ctgccctgaa actgctggac
      901 cgagtccggg aatcggaacg acggtagcgg cgactcgccg gggctggagg cggcgatgaa
      961 cagtgcctgc tcgcgcgtgg agtcgaggta gtcaacgctg gagtaggcaa cgttgacaag
     1021 tagacccatc tcgccggcgc cggtatccca ccgatcgctg agcagcaggt tgccgttcca
     1081 cgacggctct tcagcctgct cccagtagac ggcgttgacg ctgcccgaga tctcgcggcc
     1141 ggcaaagtcg aacggcttgc gcgatcggac gttgatcagg ccgccgatgc cgggctcgac
     1201 caggttcgcg gtccctgact tgtagacctc cagggccgcc acagtgcccg cgccgaagtc
     1261 ctgaatggcc accaggcggt cttgcgcggt gaagatctcg cggccgttat aagtcgtgga
     1321 cacgtccagc aggccgcgca cggcgacctg cgccgcctcg cccgccgagc gggtaacctg
     1381 cacgccggtg acgcgcgcca gggattccga ggccgtcaca tccggcagct tcccgatgtc
     1441 ctcggccacg atcgcatcga cgatggagtc gctgttgcgc ttgatcgcct gcgctgactg
     1501 caaactgcgg cgaaagccgg tgacgacgac ctcatccacc accgcagcgt cgtccgtcgt
     1561 ctgcggtgca gccggcggcg tcgtctgggc aaacgccggc gccgcgagaa gcgtggccag
     1621 gatagaggcc gagcacaggg cccgtgtctt gatcgtcatg atagtctctc cccgagattt
     1681 tgtttttatg gtcgtctccg cacctgcgcc ccttcgcgcc cgtacgatcg tcttcagtgg
     1741 gccgtcgtgg tcgtcgtatt ggcggttcca gccgcgatgg accggaacag ctcggcctcg
     1801 cgatcacggt aagatttgcc gtccgtgtgc atcagatcgt gaaaccagat gggcggctcc
     1861 tgcagggtgt agggacgctc ccagctgtcc cacggcaggc gggtctggat gtcgccatcg
     1921 acaagacccc agttgaccat gcctacattc tcgcgccgcg cgatcggcag gatggtgtca
     1981 ggggtcgatc cgttgccgcg ggccagccac tccgtggcga tcaggggccg gccgtagggc
     2041 cggagctgcg cgatgcggtt ctcgaaggac tcgggccatt cgtagttgtg gaaggtgatg
     2101 atgtcggagt gggccagttg agccttctgg atggcgttca gggtcgtggc agccggggac
     2161 cagtccgggc cgacccacac gccgctggtc agcggctgaa cgggcttgcg agagcgagcc
     2221 cacgcgaagg ccttgggcaa gagttcggcg atacgatcgc tttcctgagc cagttgttca
     2281 tcgacataac ttccgccacc cgcgttgtcg ggctcgttcc agatgtccca ggcgagaacg
     2341 cgcgggtcgt ttgcgaaggc gccgacgacc ccctccacgt aggcccggaa gcgaggatcg
     2401 ttggcgcgat cgatcagatc ctcgcgcccc gggctttgaa cccaacccga gttgtgcacg
     2461 cccggaatgg ggcggtgctg cgcgcccagg acagggtcgg gatcccagca gctgtcgaac
     2521 agcacgaaga tgatcctggt gtcgtgccgg gcggcgatcg ccagaaactg gtcgagcctg
     2581 ctcttgaaac cctcggcgtc ctggctccag agcagatcgt gcagatagac ccgcatgacg
     2641 ttcatgccga acttttcgtg cgcccagccg agttcgcgat cgatggtcac cgggtcgaag
     2701 gtttcggcct gaaacatctc aagctggttg atggcggtcg agggtatgaa gttcgccccc
     2761 accaaccacg gctggcgcgc ataccagtcg ttggcctgct gcggcgtcca gcggtcaggc
     2821 gcgcctgcga cggctgctgc gatcgctcgc cgggacgggg cgtcctgcgc gctcgccgtc
     2881 atgggcgcgc aaagaacgaa acctgcggcc agaaccgcct tgatcaggga atgacgcatg
     2941 ctgaagacct gaccttctag gaccggggac gggcggagaa acggaacacc atgtggttcc
     3001 ggtagtgctg gccggggtcc agccgcactg agccgaactc gggtcggttg ggcgtgtccg
     3061 ggaaggcctg tggctcgagg acgatggcgt cacccatgcg atacagatgg ccggacttgc
     3121 caatgactgt gccgttgaag aaattgccgg aatagaactg cacacccggc tggtcagaga
     3181 ccacttccat ggtgcggccc gagacggggt cttcgagggt ggcgatcaca cgcggctggg
     3241 ccgagcgctc gcggctgatg acccagttgt ggtcgtagcc tcgcccgaag acgatctgtt
     3301 cgtctgacgc gtcgcggacg cgtgcgccga cggcggtcgg agtacggaag tcgaacaccg
     3361 tcccctccac ggaacggaac tcgccggtcg gaatcgcggc ggcgtcagtc ggtgtatatt
     3421 cggcggcggg aatcgtcagg acgttgccca tggctccggc cgcagcgcct tcgcccgcca
     3481 tgttccagta gccgtggttg gacatgctca cgaccgtggc cttgtccgtg gtcgcgccat
     3541 agtcgacgga gagttcgccg ttgtcgttca gggaataggt ggccgtcgcg gtcagcgtac
     3601 ctggatagcc ctgatcgcca tccggactga cgtagcgcag cgtgacactg gcgtcgggcc
     3661 cgctctttac gtcgaggacc tcccatagaa ccttgtcgaa gcccttggcc ccgccgtgca
     3721 gggtgttctc accgtcgttc ttcgacacct gataggcaac gccgtcgagg gtgaaccgtc
     3781 cccccgcgat ccggttcgcg acacggccta cggtcgcgcc gaaaaactcc ggtttggcca
     3841 gatagcccgc gatggtcggg tacccgagag cgacatccgc catcaccccg tcacgatcgg
     3901 ggacgaccac cgactgaatc gcagcgccca gactgatgat ccggaccacg acgccggcat
     3961 cgttggtcag ggtgaccgcc tcgaccgccc gtccatcggc catcttgccg aattcggtgc
     4021 gaaccgcctc gcccgcaaac acaggggtgg cggacatcaa gacggcgaca agggtgagac
     4081 agcgcgtggc tgcggttcgc ataacatctc ccggcttcgt tcaaaatcag tcatctagtg
     4141 gaccgttgcg acattcatat gttaaatggt tttcattggc aatactgaag gagggttcag
     4201 tgccgctgtt ccgacatggt ttgtttctcg ccgccgcgat gtttgtgatg ggcttcagcg
     4261 atgctcgccc gggcccgcag gcgcctgtgc tggatcgcga attcgccgat cctttcgtgc
     4321 ttcccgtcga cggaggtctg gtcgcctacg caaccaatgt ccgggagaga ggaagaggga
     4381 tgaacgtcca gctgtcctca tcaattgatg ggcggacatg gtcgccaccg cgcgacgcca
     4441 tgcccacccg tccgccctgg gccctgcaac ggtctccgga tgtctgggcg cctgaagtca
     4501 tgcggatcgg ggagcgatac gtcctgttct tcagcgcgcg gcacgcgacc cgccggaggc
     4561 cggacggcct gaccctgtgt gtgggcgcag ccacgtccga tagtccggaa ggtcccttcg
     4621 ttccgcaggc cgagcccctg acctgcggcg gccccctcgg cgtcatcgac gccagcccgt
     4681 tccgggacgg ggatgatctc tggctctacg taaagagcga cggcaactgc tgccgcatcc
     4741 cgatcaccat tgtcgcccag cggctttcgt ccgacggcct cgcccttcag ggtcaaccga
     4801 ccacgatcgc cggtctgagc aacgatgcgc cgtgggaagg ccgggtgatc gaggcccagc
     4861 agatggtgct tcacgacggc cgctacatca tgttctacgc cgcgaacgac tacggtggcc
     4921 gcgactacgc caccggttac gcagtctgtg agggtccggc ggggccctgt cgggacgcac
     4981 cagagaaccc gatcctgaaa tccacatccg gtctgcaggg gctcgtcggg cccgggcatc
     5041 agagcgtgtt ccagtggaaa gatcgaacat ggatcgcatt ccatggatgg cgacgccccc
     5101 tgaccgacgg tcgctggggt tatcgctcac tctacgtctc ccccctcgac tgggtgaacg
     5161 gcagccccgt cgtcagcagc ccgaccccgt gatcgcccga gcaaaacgtg cggagcgcct
     5221 tccgctcagt tcgctgcagc ccaccccgga taggcgtcga gcgcggcttg cggcgaagcg
     5281 atccaccacg agtatccatt gcggcgacct accgagatgc cgttgacgtc gtcgtggata
     5341 gacgcgtcct tgtcgccgaa gatcggctgg ccagtgacga cgtcatagtt ccgcgaccag
     5401 atcgggcctg cgcccgcctt gtcgatcagc ttgcgaccat cgtcgg
//
LOCUS       DASVTM010000123         6448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_65771, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000123 DASVTM010000000
VERSION     DASVTM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65771"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..2112)
                     /locus_tag="VF633_03500"
     CDS             complement(58..2112)
                     /locus_tag="VF633_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470155.1"
                     /translation="MYQDVPPAPTPLPEIIVTSARLPPAASDAAFSVIRLDQTVLDRS
                     TRLDEALATVPAVSLFRRTSSLGANPTTQGISLRAIAPSGAGRTLVTLDGVPLNDPFG
                     GWVLWSQVAPESLDSLDIVRGAGAGPYGAGALTGVIALRERDREGGALDASISEYGGQ
                     RLAAATTLKSGRVAITGSVLYDHSDGYTPVRGPAAGAADTPLDLVTKSAALRADIGID
                     NLTALSLRASAYDEDRGSGLLNTRSAASGQSYSATLSRTPSNGALGWRAQVWRRESDF
                     ANSSGSVAAGRGGTTPANDQYATPASGWGTNLALRQSAIDLFGGKLEWEVGADARFSE
                     GETQERFNWNAALSQFNRDRYAGGETSVIGAYVDGSWTSGPWLVAGGLRIDQWKNEEG
                     HRLELERATGAILLNQTYPDRSGEVTSARLAVRRDLGNGFAARAAAYSGFRPATINEL
                     HRPFRVGNDITEANAALEPEKLKGVEAGLSWDHDSIWGDITFGATLFWNEIEDAIVNV
                     TIGTGPGIIAALPQGGFVPAGGVLRQRQNAGTIEATGVELNAEHRFDRITLVAAVSWT
                     DAELDGGSAAPQLTGLRPAQAPEWSATAGIDWRTTDRLTLSSRARYESARFDDDLNSR
                     VLDSAVTFDARADYRIKEGVTVYAVAENLFDEDVEVSETGDGVAGYGPPQTVRAGVSL
                     SW"
     gene            complement(2203..3237)
                     /locus_tag="VF633_03505"
     CDS             complement(2203..3237)
                     /locus_tag="VF633_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8470156.1"
                     /translation="MNRAQAFAANAPLMGVPGASSPPGGQGEWFRGAGGLRLRAAFWT
                     PSALVAAKPRGTVILSPGRTEPIEKYFEVIGNFLARGFCVLTHDWRGQGLSARLLPDR
                     LKGHARAVEEFLDDYHRLLDTFESRAPKPWIMVGHSMGAVLNLLTLENGEERFSGAVL
                     SGPMLRIKTGKRSMWSVKLAVRWNLRHGKAGDFVLDDADDPFDHTFEKDALTTDETRY
                     ELWRQQLYACPHLAVGGPTWGWLAFGLDAGERALKPKALKTVKIPCTFVQAGEDDRVW
                     KQTNKWAAKRLGRGRYVEVPGAKHEVIMETDDLRAHFLSEFDAMSDYITPIQDLSPLV
                     QPEDIEVGAA"
     gene            complement(3234..4145)
                     /locus_tag="VF633_03510"
     CDS             complement(3234..4145)
                     /locus_tag="VF633_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8470157.1"
                     /translation="MVGGSRRSLLAGLIGCAALAPLGLAACDAKPKPAAKVETPEPAA
                     PAGPPEGSLEWAIQGPWRAFDRTRDRYRHPMETLRFFGLQPNMTVVDFWPGSGWYTEI
                     LAPYLSRGGGTYYAAGFSTGPGADPAQAALMANYENRYTADKKLYGEIKFSAFGPTST
                     EVAPPGSADLVLFMRNINAWMATGIAEKAFADAFQALRPGGVLGVEQHRLAPDENQDP
                     AAANGYVQEAFVKQLAAEAGFVFLASSEINANETDTKDHPFGVGTLPPQNLTAPAGQA
                     PDPTFDRSKYDAIGESDRMTLKFRKPE"
     gene            complement(4293..5105)
                     /locus_tag="VF633_03515"
     CDS             complement(4293..5105)
                     /locus_tag="VF633_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8470158.1"
                     /translation="MRLIALTLCTAIPLMALAAPVLAQDMPTTAETPAQRASRRAAMP
                     PPVLQEDEALQAAIGADTRPAADVARDSQRHPLETLTFWGLTPATTIVEIEPGQGGWW
                     RHILEPYAAATGGTYVPVAKPLEGMGVADGTADMVVVARAFHNWAQAGRTEPYLAAFF
                     KALKPGGILAVEQHRSAEGLNVATTAPTGYVPQSYVIRAAQAAGFVLEARSEVNANPK
                     DDHDHPFGVWSLPPIRQSQARDGTGALSVEQRAELDAVGESDRMTLRFRKPF"
     gene            5359..5826
                     /locus_tag="VF633_03520"
     CDS             5359..5826
                     /locus_tag="VF633_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX8470159.1"
                     /translation="MRTIDFTPLYRSAVGFDRLARQLESATGAAEANGWPPYNIETTA
                     ENTYRVEIAVAGFKPEELNIEVKENLLTVTGRKTANDDAAPQKTYLHRGLAERDFERR
                     FQLADYVVVEDAQLDNGLLAISLKRELPEALKPRRIEIGTGKTALIEGESAAA"
     gene            complement(5882..>6448)
                     /locus_tag="VF633_03525"
     CDS             complement(5882..>6448)
                     /locus_tag="VF633_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8470160.1"
                     /translation="ALTDKGSAGAPVANLPAADMLHEHAVWCESGGAQGQPSVFDGVD
                     LRALGSITGLNLTALSAKGGVFYVLDMEGVKLQGAHLEGADLRSTRLRRADLRGARLT
                     GARLSGADLREAQLGPLMLGPDRLLPADLTGASLRGADLSGADLRRAVLQDADLSRAT
                     LHGAQTRHADLTNANLIGLRGYVPSDDV"
BASE COUNT         1072 a         2180 c         2090 g         1106 t
ORIGIN      
        1 ttgcaataga cgtagcggtc aaaagcgccg ctgcgtcatc atgcgccccg attttattca
       61 ccagctcagg ctgactccgg cccgaacggt ttgcggcggg ccgtagccgg cgacgccatc
      121 gccagtctcc gacacctcta cgtcctcgtc gaacaggttc tccgcgacgg catagaccgt
      181 gacgccttcc ttgatgcggt agtcggcgcg ggcatcgaag gtaacggcgg agtccagcac
      241 gcggctgttc aggtcgtcgt cgaagcgggc gctctcatag cgggcgcggc tcgacagtgt
      301 caggcgatcg gtcgtgcgcc agtcgatccc ggcggtcgcg ctccactcgg gcgcctgggc
      361 cggacgcagg ccggtcagct gcggcgcggc tgatccgcca tccaactcgg cgtccgtcca
      421 ggacaccgcg gcgaccagcg tgatccggtc gaagcgatgt tcggcgttga gttcgacacc
      481 ggtcgcctcg atggtcccgg cgttctggcg ctgacgcagc acgccgcccg caggcacgaa
      541 accgccttgc ggcaaggcgg cgatgatccc cggccccgtc ccgatcgtca cgttgacgat
      601 ggcgtcctcg atctcgttcc agaacagggt cgcgccgaag gtgatgtccc cccagatcga
      661 gtcatgatcc cagctcaggc cggcctcgac gcccttgagc ttttcaggct ccagcgcggc
      721 gttcgcctcg gtgatgtcgt tgccgacccg gaacggccgg tgcagttcgt tgatcgtcgc
      781 aggtcggaac ccggaatagg cggcggcgcg ggctgcgaac ccgttgccca gatcgcgacg
      841 caccgccaga cgcgccgagg tgacctcgcc cgaccggtcg ggataggtct ggttcagcag
      901 gatcgcaccc gtggcgcgct cgagctccag ccgatgccct tcctcgttct tccattggtc
      961 gatacgcagg ccgcctgcga ccagccaagg gccgctggtc cacgatccat cgacataggc
     1021 gccgatgacc gaggtctcgc ccccggcgta gcggtcgcgg ttgaactggc tcaacgcggc
     1081 gttccagttg aaccgctcct gcgtctcgcc ctcgctgaag cgggcgtcgg ctccgacttc
     1141 ccattccagt ttcccgccga acaggtcgat ggcgctctgg cgcagcgcga ggttggtccc
     1201 ccagcccgag gccggcgtcg catactgatc gttggccggg gtcgtcccgc cccgtcccgc
     1261 cgcgaccgag cccgacgagt tggcgaagtc gctctcgcgc cgccagacct gcgcccgcca
     1321 gccgagcgcg ccgttcgatg gcgtcctcga cagcgttgcg gaatagctct gcccgctggc
     1381 cgcagaacgg gtgttcaaca ggcccgatcc gcgatcctca tcataggccg aggccctcag
     1441 cgacagggcg gttagattat cgatcccgat gtctgcccga agcgcggcgc tcttggtcac
     1501 cagatccaac ggcgtgtcgg cggcgcccgc agccggtccg cgaaccggcg tgtagccgtc
     1561 cgaatggtcg taaagcaccg agccggtgat cgccacgcga ccgctcttca gcgtagtcgc
     1621 agcggccagc ctctgtccgc cgtattcgct gatcgaggcg tccagcgctc cgccttcgcg
     1681 gtcacgctca cgcagcgcga tcaccccggt cagggccccg gcgccgtaag gacccgcacc
     1741 cgcaccgcgc acgatgtcga ggctatcgag gctttcaggc gcgacctgtg accacagcac
     1801 ccaaccgccg aacggatcgt tcagaggcac accatccagc gtcaccagtg tccgacctgc
     1861 gcctgatgga gcgatggccc gcagcgaaat cccttgcgta gtcggattgg ccccaaggct
     1921 cgacgtccgc cggaacagcg acaccgccgg caccgtcgcc agcgcctcgt ccagccgggt
     1981 cgaacgatcc agcaccgtct gatcgagcct gattacagag aatgccgcgt cgctggcggc
     2041 aggcggcaat cgcgccgacg tgacgatgat ctcgggcaac ggggtcgggg cgggcggaac
     2101 atcctggtac atgggcgctc caatacacgg cgcctgcggg ggttgcgttg gcctttggtt
     2161 ctttttgccc caaaaacgtc ttcaggctcg ccctcaatgg gctcaggcgg cgccgacctc
     2221 tatgtcttcg ggctggacca gggggctcag gtcctgaata ggcgtgatgt agtcggacat
     2281 ggcgtcgaac tcggagagga aatgggccct caggtcgtcg gtctccatga tgacctcgtg
     2341 cttcgctccc gggacctcga catagcgacc ccgtccaaga cgtttcgccg cccacttgtt
     2401 ggtctgtttc cagacccggt cgtcctcgcc cgcctgaacg aaggtgcagg gaatcttgac
     2461 cgtcttcaat gctttgggtt tcagcgcccg ctcgccggcg tccaacccga atgccagcca
     2521 gccccacgtc gggccgccca ccgccaggtg agggcaggcg taaagctgct gccgccacag
     2581 ttcgtagcgg gtctcgtccg tggtcagggc gtccttctcg aacgtgtggt cgaagggatc
     2641 gtcggcatcg tccagaacga aatctccggc cttgccgtga cgcaggttcc agcgaacggc
     2701 cagcttgacg gaccacatcg atcgcttgcc agtctttata cggagcatcg gtccggacag
     2761 caccgcgccg gagaaccgct cctcgccgtt ttccagcgtc agcaggttca gcacggcgcc
     2821 catcgaatga ccgaccatga tccagggctt gggcgcgcgg gactcgaacg tgtccagcag
     2881 ccggtgatag tcgtccagaa attcctcgac cgcccgggcg tggcccttca gccggtcagg
     2941 cagcagccgc gccgacagcc cctgaccgcg ccagtcgtgc gtcaggacgc agaagccgcg
     3001 ggcgaggaaa ttgccgatga cctcgaaata cttctcgatc ggctcggtcc gccccggcga
     3061 caggatcacg gttccgcgcg gtttggcggc gaccagagca gatggcgtcc agaaggcggc
     3121 gcgcagccgg aggccgccgg cgccccggaa ccactcgccc tgtccgccgg gaggcgacga
     3181 ggcgccgggt acgcccatca gcggggcgtt ggcggcaaag gcttgggcgc ggttcactca
     3241 ggtttcctga acttcaaggt catgcggtcg ctctcgccga tcgcgtcgta tttgcttcgg
     3301 tcgaacgtcg gatcgggggc ctgacccgca ggggcggtca ggttttgcgg cggaagggtc
     3361 ccgacaccga acggatgatc cttggtatct gtttcattcg cgttgatttc tgaagacgcg
     3421 aggaagacaa aacctgcctc tgcggcgagt tgcttgacga aggcctcctg aacatagccg
     3481 ttggcggcgg ctggatcctg gttctcgtcg ggcgccaggc gatgttgttc gacgcccagc
     3541 acgccgccgg gtcgcaacgc ctggaaggcg tcggcgaatg ccttctccgc tatgccggtg
     3601 gccatccatg cgttgatgtt gcgcatgaac agcacgaggt ccgctgatcc cggcggcgcc
     3661 acttcggtcg aagtgggccc gaaagcggag aatttgattt ctccgtacag cttcttgtcg
     3721 gcggtgtagc ggttttcata gttggccatc agggcggcct gagcgggatc ggcgccgggc
     3781 cccgtgctga agcccgccgc gtaataggtt ccgccgccgc gcgacagata gggcgccaga
     3841 atctcggtgt accaaccgct tcccggccag aagtcaacga cggtcatatt gggctggagt
     3901 ccaaagaacc gcagggtctc catcggatga cgatagcgat cacgggtgcg gtcaaaggcg
     3961 cgccagggtc cttgaatagc ccattccaga gaaccctccg gcggtccggc aggcgctgcg
     4021 ggctcgggcg tctcgacctt ggctgcgggt ttgggtttgg cgtcgcaagc ggcgaggccg
     4081 agcggcgcca gggctgcgca gccaatcaga ccagccagca gggatcggcg tgagcctcct
     4141 accatttcag gcctccgttc ggacggctcg cgttcatgca tacgggtctc ccgccgagcc
     4201 gtatgaccta cggccttgct cgggtcgaac gggattaacg ggcttcgttg cgtgcgtcaa
     4261 agccccgctt tgggcgggat cgacagggtg tttcagaaag gctttctgaa tcgcagcgtc
     4321 attcgatcac tctcgccgac agcgtccagt tcggcgcgct gttccacgct cagggcgccg
     4381 gttccatcac gcgcctgaga ctggcgaatg ggaggcaggc tccacacccc gaacgggtgg
     4441 tcatgatcgt ccttcggatt ggcgttcact tcgctgcgcg cctccagcac gaagcctgcc
     4501 gcctgcgcgg cccggatgac ataactctgg ggcacatagc cggtcggggc ggtggtcgcg
     4561 acattcagtc cctcggcgct gcgatgctgc tcgacggcca gaatgccgcc cggcttcagt
     4621 gccttgaaga aggccgccag atagggctcg gtgcggccag cttgagccca gttatggaag
     4681 gcgcgcgcca cgacgaccat gtcggcggtt ccgtcggcta cgcccatgcc ttccagcggc
     4741 ttggcgacgg ggacataggt cccgccggtt gcggctgcgt agggctcgag gatatgccgc
     4801 caccagccgc cctgacccgg ctcgatctcc acgatcgtcg tcgcgggcgt caggccccag
     4861 aaggtcaggg tttcaagagg atggcgctgg ctgtcgcgcg caacatcggc ggccggacgg
     4921 gtatccgctc cgatcgctgc ttgcagagcc tcgtcttcct gcagaaccgg cgggggcatc
     4981 gctgcgcggc gagatgcgcg ttgagcgggc gtctcggctg tggtcggcat gtcctgagcc
     5041 aggacgggag ccgccaaagc cataagggga atggcggtgc agagcgtgag ggcgatcagg
     5101 cgcatggtcg tctccgaaag aacccgtcgt cagtacccgc tcgcctcgcc aaaacaaagg
     5161 cgacgaaacg tccgaaaaag catgaccgcg gccctcttgc gcgccgaaaa ccgctaccca
     5221 catcctcaat cgaaggcgcc gattatcggg ccttctcaga ccggcggggc ccattcggga
     5281 ccgctgacct gacatcccac cgccgttccg ggcaaaaacg cgggacggtt ccatcttgct
     5341 gatcatcagg agatcgacat gcgtaccatc gactttaccc ccctctatcg ttcggccgtc
     5401 ggcttcgacc gtctggcccg ccagctagag agcgccacgg gcgccgcaga ggccaatggt
     5461 tggcccccct acaacatcga gacgacggct gagaacacct accgcgtcga gatcgccgtg
     5521 gccggcttca agcccgaaga gctgaacatc gaggtcaagg aaaacctcct gaccgtcacc
     5581 ggccgcaaga ccgccaacga cgacgccgct ccgcagaaga cctatctgca ccgtggcctc
     5641 gccgagcgcg acttcgagcg ccggttccag ctggctgact acgtcgtggt cgaagatgcc
     5701 cagctcgaca acggccttct ggccatcagc ctgaagcgtg agctgcccga ggccctcaag
     5761 ccccgccgca tcgaaatcgg caccggaaag actgcgctga tcgaaggcga atccgccgcc
     5821 gcttaagcgg caggcgttaa ccgaagacct tggagggcgg cgcgaaagcg tcgccctttt
     5881 cttatacgtc gtcgctcggg acgtagcccc gcaggccaat caggttggcg ttggtcaaat
     5941 cggcatggcg ggtttgtgcg ccgtgcaggg tcgcgcggct gagatcggcg tcctgcagca
     6001 ccgctcgccg aagatctgcg cccgagaggt cggctccccg caatgaggct ccggtcaggt
     6061 cggcgggcag cagcctgtcg ggccccagca tcaatggccc cagttgagcc tcacgcaggt
     6121 cggcgccgga aagccgcgct ccggtcagtc gtgcgcctct gaggtcggcc cgccgcagac
     6181 gtgtcgagcg caggtcggcc ccctccagat gtgcgccctg aagcttcacc ccttccatgt
     6241 cgaggacgta gaagaccccg cccttggccg acagggccgt caggttcagg ccggtgatcg
     6301 accccagcgc ccgcaggtcg accccgtcga acaccgaggg ctgaccctgt gcgccgccgc
     6361 tttcacacca gacggcatgt tcgtgcagca tgtcggcggc aggtagattg gccaccggcg
     6421 cgccggccga gcccttgtcg gtcaacgc
//
LOCUS       DASVTM010000124         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_915452,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000124 DASVTM010000000
VERSION     DASVTM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915452"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..696
                     /gene="rimP"
                     /locus_tag="VF633_03530"
     CDS             82..696
                     /gene="rimP"
                     /locus_tag="VF633_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX8470161.1"
                     /translation="MRAKTTQDRQLLELIDPVAESLGLEVVRVRLMGGTLRQRLQIMA
                     ERPSDHDISVEECARLSRAVSEIFDAADPIAGEYLLEVSSPGIDRPLTRQIDFELFEG
                     FEARLESDRMIEGRKRFKGLLAGLDKDDAGNVLVAIDLDGEEDTALIPFDWMVDAKLV
                     MNDDLIKRGALLRAARGEPEDDGLPEGAPEDNDTTPQTLPEDKA"
     gene            696..2468
                     /gene="nusA"
                     /locus_tag="VF633_03535"
     CDS             696..2468
                     /gene="nusA"
                     /locus_tag="VF633_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX8470162.1"
                     /translation="MAVTGISANRLELLQIANAVAQEKNIDREIVIEALEEAIQKGAK
                     SRYGAHHDIRARIDPKTGELALTRHVTIVDNDWQPEDELEEFNDSAMVRLNDASKRDP
                     EAEVGKEYVESLPPFEFGRVQTQMARQVITGKVRDAERANQYEEFKDRVGEIVNGTVK
                     RVEYGNTIVDLGRGEGIMRRDQSIPREIFNIGDRIRTYIYDVRPEAKGPQIMLSRAHP
                     GFMAKLFAQEVPEVYDGVIEIRAAARDAGSRAKMAVLSNDSSIDPVGACVGMRGSRVQ
                     AVVAELQGEKIDIIQWNADEPTFIVNALAPAEVSKVVLDEEADRVEVVVPDEQLSLAI
                     GRRGQNVRLASQLTGWQIDIITESQDSERRQREFSERTLLFQEALDVDEVIAQLLVTE
                     GFATVEDLAFVENYEVAEIEGFDEDTAEELQARARDFLERQAAALDTKRVELGVLDEV
                     MAVPGVTGAIAVALGEGGIKTVEDLADLATDEIRGGYEMKNGERTKLPGVLESFNMSQ
                     EDAEMLILQARVAAGWIDASELPQTEPEYEEELPEGEYDADAVFGDAPAQSSEEGSEG
                     LEEATLDAGDAEVFAEDLESSRDA"
     gene            2487..3164
                     /locus_tag="VF633_03540"
     CDS             2487..3164
                     /locus_tag="VF633_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX8470163.1"
                     /translation="MSLRDATTDRERRDLVTHEVMDESRLIRFVAGPDGSVFPDLGRK
                     LPGRGLWVAADRASVETAAKKNLFSRAAKTKLNASADLAAVVESQLFRRCLDQLGLAR
                     REGVLISGFEKSAASIRAGKAAWVIEASDGASDGRGKILALARHQTTKVCGAFSVDDL
                     SLALGLENAIHGVLLHGGRADRWTTEVERLAGFRSLRPAAWDLDFRPHAVDGPTEDLK
                     DEPERAD"
     gene            3285..>3695
                     /locus_tag="VF633_03545"
     CDS             3285..>3695
                     /locus_tag="VF633_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HEX8470164.1"
                     /translation="MSDENDKTNEGQDAVAPAGTPSTTGPRAPLSLKPRVAGSVSTGT
                     VRQSFSHGRTKTVVVETKRRPGAPVGGHQRPQGFDVSRPRAEVAPASQAPRPPMRQPA
                     GGSLSNEEQEARRRAIESATRAQADRAAAQAAEQA"
BASE COUNT          714 a         1198 c         1194 g          589 t
ORIGIN      
        1 cgttagacat cggcgtccaa cggcgccgtc tccaagcggc ggccggacgg ccagccgctt
       61 ttttgttgag ttattcccgc cttgcgcgcc aaaaccaccc aggaccgcca gctgctagag
      121 ctgatagacc ccgtcgccga aagcctcggc ctcgaagtcg tgcgtgtgcg tctgatgggc
      181 ggcaccctgc gccagcgctt gcagatcatg gccgagcgcc cgagcgacca cgacatctcg
      241 gtcgaggaat gcgcccgcct gagccgcgcg gtttccgaaa tcttcgacgc cgccgacccc
      301 atcgccggcg aatatctgct tgaagtgtcc tcgcccggca tcgaccgtcc cctgactcgt
      361 cagatcgatt tcgaactgtt cgagggtttc gaggcgcgtc tggaatccga ccgcatgatc
      421 gaggggcgca agcgcttcaa gggtctgctg gccgggctgg acaaggacga cgccggtaac
      481 gttctggtcg ctatcgacct ggacggcgag gaagacaccg ccctgatccc gttcgactgg
      541 atggtggacg ccaagctggt catgaacgac gacctgatca aacgcggcgc gttgctgaga
      601 gcagcgcgcg gcgaacctga agacgacggg cttcccgaag gggcgcctga agacaacgac
      661 acgacccccc agacacttcc tgaggacaaa gcctgatggc cgtcaccggt atttccgcta
      721 accgtctcga acttctccag atcgccaacg cggtcgctca ggagaagaac attgatcgtg
      781 agatcgtcat cgaggcgctg gaagaagcga tccagaaggg cgcgaagtcg cgctacggcg
      841 ctcatcacga catccgcgcc aggatcgacc ccaagaccgg cgagctggcc ctgacccgtc
      901 acgtcaccat cgtcgacaac gactggcagc ctgaagacga gctggaggag ttcaacgata
      961 gcgccatggt gcgcctgaac gacgcctcca agcgcgaccc ggaagccgaa gtcggcaagg
     1021 aatacgtcga gagcctgccg ccgttcgagt tcggtcgcgt tcagacccag atggcccgtc
     1081 aggtcatcac cggtaaggtc cgcgacgccg agcgcgccaa ccagtacgaa gagttcaagg
     1141 accgcgtcgg cgagatcgtc aacggcaccg tcaagcgcgt cgaatacggc aacaccatcg
     1201 tcgatctggg ccgtggcgaa ggcatcatgc gccgcgacca gtcgatcccg cgcgaaatct
     1261 tcaacatcgg cgaccggatc cgcacctaca tctacgacgt ccgtccggag gccaagggcc
     1321 cgcagatcat gctcagccgc gcccatcctg gcttcatggc caagctgttc gcgcaggaag
     1381 tgccggaagt ctacgacggc gtcatcgaga tccgcgccgc cgcccgcgac gccggttcgc
     1441 gcgccaagat ggccgtgctc tcgaacgaca gctccatcga tcctgtcggc gcctgcgtcg
     1501 gtatgcgcgg ttcgcgggtt caggcggtcg tcgccgaact gcagggcgag aagatcgata
     1561 tcatccagtg gaacgccgac gagccaacct tcatcgtcaa cgccctggcg cctgctgaag
     1621 tctccaaggt cgtgctcgac gaagaagccg atcgcgtcga agtggtcgtg cctgacgaac
     1681 agctgtcgct ggccatcggc cgtcgcggcc agaacgtacg cctcgcctcg caactgaccg
     1741 gctggcagat cgacatcatc acggagtcgc aagactccga acgtcgccag cgcgagttct
     1801 cggaacgcac cctgctgttc caggaagccc tggacgtcga tgaagtcatc gcccagctgc
     1861 tggttaccga aggcttcgcc acggtcgagg atctggcctt cgtcgaaaac tacgaagtcg
     1921 ccgaaatcga aggcttcgac gaagacaccg ccgaggaact gcaggcccgc gcccgtgatt
     1981 tcctcgaacg tcaggctgcc gcgctggaca ccaagcgtgt cgaactcggc gtgctggacg
     2041 aagtcatggc cgttccgggc gtgaccggcg ccatcgccgt cgccctgggc gaaggcggga
     2101 tcaagaccgt cgaggatctg gccgatctgg cgacggacga aatccgcggc ggctacgaaa
     2161 tgaagaacgg cgagcgcacc aagctcccgg gcgtgctgga aagcttcaac atgtctcagg
     2221 aggacgccga gatgctgatc cttcaggcac gggttgcggc cggatggatc gacgcttcgg
     2281 aactgccgca gaccgagccg gaatacgagg aagagcttcc cgagggtgaa tacgacgccg
     2341 atgccgtgtt cggcgacgcc ccggctcaat cttcggaaga gggttccgag ggtctcgaag
     2401 aagcgacgct ggacgccggc gacgcggagg tcttcgccga agacctcgaa tcttcccgcg
     2461 acgcgtgatg ggaagcggga ccgatcatga gcttgcggga cgcaacgacg gatagagagc
     2521 gccgggacct cgtcactcac gaggtcatgg atgaatctcg tctgatccgt ttcgtcgccg
     2581 ggcctgacgg ttccgtgttt ccagacctgg gcaggaaact gcccgggcgc ggcctctggg
     2641 tcgccgccga ccgcgcctcg gtcgagacgg cggccaagaa gaacctgttc tcacgggccg
     2701 ccaagaccaa gctcaacgcc tccgccgacc ttgccgctgt ggtcgaaagc cagctgtttc
     2761 gtcgctgtct cgatcaactg ggccttgccc ggcgcgaagg cgtgcttatc tcaggcttcg
     2821 aaaagtcggc cgcgagcatc cgcgccggca aggccgcgtg ggtcatcgag gcgtccgacg
     2881 gcgcctctga cggacgcggc aaaatcctcg ctctggcgcg acatcagacc acaaaggttt
     2941 gcggcgcgtt cagcgtggac gatttgagtt tggcccttgg gctggaaaat gcgatacatg
     3001 gcgtcctgct gcatggcggg cgcgctgatc gctggaccac cgaggtcgaa cgactggcgg
     3061 gatttcgatc gcttcgccct gcggcgtggg acctggattt caggccgcac gctgtggacg
     3121 ggccgaccga ggacctgaag gacgagcctg aacgggcgga ttgacgggcg gggtctggcg
     3181 ttttccaacg ccgggctgcg cacggctttt tgtgttttcg gactttggtg acgagacgat
     3241 ttcaagatca ggcggacttc cccgccacga gtaaaagcga ccggatgagc gacgagaacg
     3301 acaagaccaa cgaaggccag gacgccgtgg cgcccgcagg aacaccgtcg acgacgggtc
     3361 cgcgcgcgcc gctgagcctg aagccgcgcg tcgcgggatc ggtttcgacc ggcaccgtac
     3421 gccagagctt cagccatggc cgcaccaaga cggtggtcgt cgagaccaaa cgtcgtccgg
     3481 gagcgccagt cggcggtcac cagcgtccgc agggcttcga tgtttcgcgt ccgcgcgccg
     3541 aggtcgctcc tgcgtcccag gctccgcgtc cgcctatgcg tcagccggcc ggcggaagcc
     3601 tgtcgaacga ggagcaggaa gcccgccgcc gcgccattga gtcggccacg cgagcccagg
     3661 ccgatcgcgc tgctgcacag gccgccgaac aggcg
//
LOCUS       DASVTM010000125         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_758310,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000125 DASVTM010000000
VERSION     DASVTM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758310"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VF633_03550"
     CDS             <1..445
                     /locus_tag="VF633_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470165.1"
                     /translation="MAKLKVFTWADGFHAFTVAASSKPKALAAWGIGADIFKSGLASE
                     ITEGADFDAALASPGEVIERGLSIDVGKITRAAKPKPVSNKAREDVRALEADLDALDL
                     SQAAEVAELDARRAALDAEAKSLAATQDRARKALMVRLRAARSKL"
     gene            582..1586
                     /gene="hemB"
                     /locus_tag="VF633_03555"
     CDS             582..1586
                     /gene="hemB"
                     /locus_tag="VF633_03555"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX8470166.1"
                     /translation="MTLPFAPAAFPMARPRRLRSQPWVRRLVAETTLTPSDLIWPLIV
                     HDGKEDRVPVPSMPGVFRLSPKAAAEAAVEARSLGIPLIALFPNVDAATKDAVGTGAT
                     DPDGLIPDCIKAIKDAAPEIGVMCDVALDCYTDHGHDGIVENGRIMNDASLDRLSEQA
                     FIQAHAGADIVAPSDMMDGRVQAIREALEANGFHDTLILSYAAKFASAFYGPYRDAVG
                     SAKMLTGDKKTYQMDYANADEALREVAMDIAEGADAVMVKPGMPYLDIVRIVADTFKV
                     PTFAYQVSGEYSMMQASIANGWLDEDRAILETLHGFKRAGCAGVLTYFAPKAARLLGA
                     "
     gene            1822..>2888
                     /locus_tag="VF633_03560"
     CDS             1822..>2888
                     /locus_tag="VF633_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470167.1"
                     /translation="MLDDNRTDVPADQLGPLVAKYSNIFLEVRWVFPRRYDQLSHYAW
                     LLSDPGADELDTVELAHLSRELQTRLFGTGLEDAVKLVLFEGDDEAIETFSAWPAETV
                     LDALQNPDGLPPGCRLRRIAPDGSLSGPEETGETPGEPDTPNLFKFAPTIDTAQGVYF
                     PPGRQFVGDVLSCTPVSAPTYYSLLDGADHRPEDTEAFDAACVTTALRFLVDFPSLPR
                     LFVPISFSTLVRPSQRAAYLDLIMVLPPIFRSRLSASVYDVPRSPTFQALKTLREMLD
                     PHFGAIGLGTHDPDFQIEQLAERAVHSVTFTLPDAHPDVRLAALRRFASHTPHYRKRC
                     IASGVTNIRFRTERELAIELKV"
BASE COUNT          496 a         1044 c          858 g          490 t
ORIGIN      
        1 aatggcgaag ctgaaggtct ttacgtgggc cgacggcttc catgccttca ccgtggccgc
       61 ctcgtcgaag cccaaggcgc tggccgcatg gggcatcggc gccgacatct tcaaaagcgg
      121 tctggcgtct gagatcaccg agggcgcaga tttcgacgcc gccctcgcct cgcccggcga
      181 ggtcatcgaa cgcggcctgt ccatcgacgt gggcaagatc accagggctg caaagcccaa
      241 accggtctcg aacaaggcac gcgaggacgt cagggcgctg gaagccgacc ttgatgcgct
      301 ggacctgagc caggcggcgg aggtggccga actcgatgcg cgccgcgccg cgctggacgc
      361 cgaggcgaaa tcgctcgcag cgacacagga cagagcccgc aaggcgctga tggtcaggct
      421 gagggcagca cgctcaaaac tctagatcgc gtcgaacaga gagggggagc gacgatgcct
      481 gcgccacaca acggtttgcg gagcggccgc aactcgagac agcctgtgcg acagcagagg
      541 ttggccttct cccgccccaa gaaggagcct agatgaggct catgaccctc cccttcgcgc
      601 ccgccgcctt ccctatggcc cgtcctcgcc gcctgcgcag ccagccgtgg gtgcgtcgtc
      661 tggtggccga gaccaccctg acgccctccg acctgatctg gccgctgatc gtacatgacg
      721 gcaaggagga ccgcgtcccg gtcccgtcga tgcccggcgt cttccgcctg tcgcccaagg
      781 ccgccgccga ggccgcagtc gaggcgcgct cgctcggcat tccgctgatc gccctgtttc
      841 ccaacgtcga cgccgccacc aaggacgccg tcggcacagg cgcgaccgac cccgacggcc
      901 tgatccccga ctgcatcaag gccatcaagg acgccgcccc cgagatcggc gtcatgtgcg
      961 acgtggcgct ggactgctac accgaccacg gccacgacgg tatcgtcgag aacggtcgca
     1021 tcatgaatga cgcctcgctc gaccgcctgt ccgaacaggc cttcatccag gcccacgctg
     1081 gcgccgacat cgtcgcccca tcggacatga tggacggccg cgtccaggcc atccgcgagg
     1141 cgctggaagc caacggcttc catgacaccc tgatcctgtc ctatgccgcc aaattcgcct
     1201 cggccttcta tggcccgtac cgcgatgccg tcggctcggc caagatgctg accggcgaca
     1261 agaagaccta tcagatggac tacgccaacg ccgacgaggc cctgcgcgag gtcgccatgg
     1321 acatcgccga gggcgccgac gccgtcatgg tcaagcccgg catgccctat ctcgacatcg
     1381 tccgcatcgt cgccgacacc ttcaaggtgc cgaccttcgc ctatcaggtg tcgggggaat
     1441 actcgatgat gcaggcctcc atcgccaacg gctggctcga cgaggaccgc gccatcctcg
     1501 agaccctgca cggcttcaag cgcgcaggct gcgcgggcgt cctgacctat ttcgccccca
     1561 aggcggcgag gctgctgggg gcgtagccgc cgctctggtc ccggagcccg cccttcgaga
     1621 tcctgtcgac cggcggccgg ttcctcgtcg aggcgctcat caccccggtg agttagctca
     1681 ggcttatcag cgactttcgt ccccgagcca cgaacccgtt cgacgggcat tcgaacccgg
     1741 cttcgccaaa tcttcattct cggcagccaa catcgtcagc ggaggacctg acgagtggcg
     1801 gcagttgcgt gtttgagagt gttgctcgac gataaccgga cagacgtgcc tgccgatcag
     1861 cttggcccgc tggtcgccaa atacagcaac atcttccttg aggttcgctg ggttttcccc
     1921 cgccgctacg accagctgag ccactacgcc tggctgttga gcgatcccgg cgccgatgaa
     1981 ctcgacaccg tcgaacttgc ccatctgtcg cgcgaacttc agacgcggct gttcggcacc
     2041 ggcctggagg acgcggtcaa gttggtcctt ttcgagggcg acgatgaagc tatcgagaca
     2101 ttttccgcct ggcctgccga gaccgtgctg gacgccttgc agaatccgga cggcctgcct
     2161 cccggctgcc gtctacgccg aatcgctccg gacggctcgc ttagcggacc agaagagaca
     2221 ggcgagacgc ccggtgagcc cgacacaccc aacctcttca agttcgcccc caccatcgac
     2281 acggcgcaag gggtctattt cccccccggc cgccagttcg ttggcgacgt gctcagttgc
     2341 actccggtct ccgccccgac ctattacagc ctgctcgacg gcgctgacca ccggcccgag
     2401 gacaccgagg ccttcgacgc cgcgtgcgtg accaccgcgc tgcggtttct cgtcgacttc
     2461 ccgagcttgc cgcgcctgtt cgtgccgatc tcgttttcca cactggtccg tccgagccag
     2521 cgagctgctt atctcgatct cataatggtg ctgccgccaa tctttcgatc ccgcctgtca
     2581 gccagcgtct acgacgttcc ccgttcgccc accttccagg ccctcaagac cctccgggaa
     2641 atgcttgacc cccatttcgg ggcgatcggc ctgggcaccc acgatccgga tttccagatc
     2701 gagcagttgg ccgagcgcgc ggtgcacagc gtgaccttta ccttgcccga cgcccacccg
     2761 gacgtgcgtc tggcggcgct gcgccgcttc gccagtcata ctccccacta tcgcaaacgc
     2821 tgcatagcgt cgggcgtgac caacatccgc ttccgcaccg agcgcgagct cgccatcgag
     2881 ctgaaggt
//
LOCUS       DASVTM010000126         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_969811,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000126 DASVTM010000000
VERSION     DASVTM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969811"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VF633_03565"
     CDS             <1..684
                     /locus_tag="VF633_03565"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX8470168.1"
                     /translation="VQIYCDFSAYSDMAIGIAALLGYSFPRNFDQPYRASSIQSFWRR
                     WHISLSSWLRDYLYVPLGGSRRGLAMSCVNVMITMLLGGLWHGAAWTFVAWGGLHGGA
                     QVVERLGRTVFGNRRVIPGWLGVLITFHIVCLGWILFRAETFDMAVAVLQGLGRAGPM
                     LMTTPFVLTLIVGGLAMHWLPPRAVEGIAERLKPAPSLTVAVLIGLAILMVEAVRPDG
                     VAPFIYFQF"
     gene            697..1200
                     /locus_tag="VF633_03570"
     CDS             697..1200
                     /locus_tag="VF633_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470169.1"
                     /translation="MTDDPEEPLIGPFPTSFPYSPFPPLPGDASVGPAQDPASDWIET
                     ADAHDLPIQDTTHPLVALSRFAFPPAPAPDEEGVAARDRRWTSRVIMLVTVFLLVFNA
                     ASIQNWSRQQAPGWITSTVQQLADVWAAQVAQLGAEQPRQAVRDAYETLHTLRFPGQK
                     PASKEER"
     gene            1269..2426
                     /locus_tag="VF633_03575"
     CDS             1269..2426
                     /locus_tag="VF633_03575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8470170.1"
                     /translation="MTAQAPKFEVEIVRATEISDRDMAAWSAIQHAQPVFRSPLLSPH
                     FARAVGRLRDDARITIVRKAAEPVAFLAFHRRPGGFGRPIGAPFSDYHGVVSTPDTGL
                     MPGQVIGLAGLKAFRHNGLIDPHGLFPVTNDAVEAFAIELNGTPDGYLEAVRAASPKK
                     FKNYRRLAHRLAEVGPLTMRTDTSQAAFDDVMAWKSAQFVRTGIQDVLRPAWVSELMQ
                     SLFETREGPMTGLLLSLYAGDTFVGGHFGVRENGVYHPWIASMSPEMAAYSPGQTFLD
                     QAIRAMPELGLTVYDLGVGHDHYKRPFAPTVDHVGAGFHTAAGGGLKRAEEAAWTLGP
                     LGRSPAIDKVRRRLDHIATADPSIRGRVHGLIEAVVATRRRSLGGEGGASE"
     gene            2423..>2911
                     /locus_tag="VF633_03580"
     CDS             2423..>2911
                     /locus_tag="VF633_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470171.1"
                     /translation="MTIITDWTADKAAQLTKANLGFDHTLHERPMFDDEGLISLLDRY
                     PRDKLGVFTMGHDPVDWQSWRRGSAGDLSGEQLLRAAQDGRIWLNLRHTNDYLPEYGL
                     LEEEIFAEKRAQSGIKTFKRDLGMLISSANAQVFYHLDVPLVSLWQLRGQKRVWVYPV
                     AEP"
BASE COUNT          485 a          931 c          930 g          565 t
ORIGIN      
        1 gtccagatat attgcgactt ctccgcctat tcggacatgg cgatcggcat cgcagccctg
       61 cttggctatt ccttcccgcg aaacttcgac cagccctacc gggccagttc gatccagtcg
      121 ttctggcggc gctggcatat cagcctgtcc agttggctaa gagactattt gtatgttccg
      181 ttgggcggga gccggcgtgg gctggccatg tcctgcgtca acgtaatgat cacaatgctg
      241 ctcggtggtc tctggcacgg ggcggcctgg acgttcgtag cctggggcgg gttgcacggc
      301 ggggcccagg tcgtcgaaag gctgggccgc acggtctttg ggaaccggcg ggtgattccc
      361 gggtggctgg gggttctgat caccttccac atcgtctgcc tgggctggat actgttccgg
      421 gcggagacct tcgacatggc cgtcgccgtg ctacagggcc ttggccgagc cggacccatg
      481 ttgatgacga cgccgttcgt gttgacgttg attgtgggag gattggccat gcactggctg
      541 ccgccgcgcg ccgtcgaggg gattgcagaa cgcctcaagc ctgcgccgtc gctgactgtc
      601 gccgtcctga tcggcctcgc tattctcatg gtggaggcgg tgcgccctga tggcgtcgcg
      661 cccttcatct atttccagtt ctgagggagg cgacagatga ccgacgatcc cgaagagcct
      721 ctgatcggac cctttccgac cagcttcccg tactcgccat tccccccact gccgggtgac
      781 gcatctgtcg ggccggctca agacccagcg agcgactgga tcgagaccgc ggacgctcac
      841 gacctgccga tacaggacac cacgcatcct ctggtagcct tgagccgctt cgccttcccg
      901 cccgcacccg cgccggacga ggagggcgtc gccgcgcgtg atcgccgctg gaccagccgg
      961 gtcatcatgc tggtgaccgt cttcctgctg gtgttcaacg ccgcctcgat ccagaactgg
     1021 tcccgtcagc aggctcccgg ctggatcacg agcacggtgc agcaactggc cgatgtctgg
     1081 gctgcccaag tcgcccaact cggcgccgag caacctcgcc aagccgttcg cgacgcctat
     1141 gaaaccctcc acacgcttcg tttcccgggc cagaagccgg cgtccaagga ggagcgctga
     1201 gttcggcagc caacactctg gcaaacacat tgcacggctc aaacagagct gtttccgagt
     1261 gtgccataat gactgcccaa gccccgaaat tcgaagttga gatcgtccgg gcaactgaga
     1321 tttcagaccg tgatatggcc gcatggtcgg cgatccagca cgcacaaccg gtgtttcgca
     1381 gccctctgct cagcccccat ttcgcccgcg ccgtcggccg gctgcgcgac gacgcccgga
     1441 taacgatcgt gcgcaaggcc gccgagccgg ttgcgttctt ggcctttcac aggcgtcccg
     1501 gcggttttgg acgaccgata ggcgcgccgt tcagcgacta tcatggggtt gtttcgacgc
     1561 ctgacacggg gttgatgccc ggccaggtca tcggcctcgc ggggctgaag gcgtttcggc
     1621 ataacggcct gatcgatccg cacggcctgt tccccgtgac gaatgacgct gtcgaggctt
     1681 tcgccataga attgaacggt acgcccgacg gctatctgga ggcggtgcgc gccgccagcc
     1741 ccaagaaatt caagaactac cgccgtttgg cgcaccgtct ggccgaagtc gggccactga
     1801 cgatgcgtac cgatacctca caagcggcgt tcgacgacgt catggcctgg aagagcgcgc
     1861 agttcgtgcg caccggcatt caagatgtgc tgcgtccggc atgggtctcc gagctgatgc
     1921 agtccctgtt cgagacgcgc gaaggcccga tgactggtct gctgttgtcg ctctatgcag
     1981 gcgacacctt cgttggcgga catttcggcg tgcgcgagaa tggagtctat catccgtgga
     2041 tcgcgtcgat gtcgcctgag atggcggcct attccccggg acagacgttc ctcgatcagg
     2101 cgatccgcgc gatgccggaa ctggggctga ccgtctatga tctgggtgtc ggccacgacc
     2161 actacaagcg ccctttcgcc ccgacagtcg atcacgtggg cgcgggtttc cacacggcag
     2221 cgggcggtgg gctgaagcgc gccgaggaag ccgcctggac cctcggaccg ctcgggcggt
     2281 cgccggcgat cgacaaggtg cgtcgccgcc tcgaccacat cgccaccgca gacccctcga
     2341 tccgtggccg ggtgcatggc ctgatcgagg cggtcgtcgc cacgcgtcgt cgctcgctgg
     2401 gtggtgaagg gggagcttcg gaatgaccat catcaccgat tggaccgccg acaaggcggc
     2461 gcagctgacc aaggccaatc tgggtttcga ccacactctg cacgaacgtc cgatgttcga
     2521 cgacgagggg ctgatctcgc tgctggaccg ctatccgcgc gacaagctcg gcgtcttcac
     2581 catggggcat gatccggtcg actggcagag ctggcgtcgg ggttcggctg gtgacttgtc
     2641 gggagagcaa ctgctgcgcg ccgctcagga cggtcgcatc tggctcaacc tgcgtcacac
     2701 caacgactat ctgccggaat acgggttgct cgaagaggag atcttcgccg agaaacgcgc
     2761 gcagtcgggc atcaagacct tcaagcgcga tctggggatg ctgatttcat cagccaacgc
     2821 gcaggtgttt tatcacctcg acgtgccgct ggtgtcgctg tggcagttgc ggggccagaa
     2881 acgggtttgg gtctatccgg tcgccgaacc c
//
LOCUS       DASVTM010000127         7829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_597529,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000127 DASVTM010000000
VERSION     DASVTM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597529"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF633_03585"
     CDS             complement(<1..518)
                     /locus_tag="VF633_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX8470172.1"
                     /translation="MNFFNNLPVGRKMFAAFAVVLTAILVMGGVVGMNLMHISRIGET
                     RRMENKVNQLISIAEFRVSKQENAMRGFLLSNNPYYLERLEAHRTAFKAALAETRGLL
                     DADRDADAGAAEAAGDAWFAKVVTPGVAMMRDPSQRAQAQALVAPDGIADEMIKPLED
                     SIEAMKSKSETSL"
     gene            complement(548..1243)
                     /locus_tag="VF633_03590"
     CDS             complement(548..1243)
                     /locus_tag="VF633_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX8470173.1"
                     /translation="MNNTVILPIVTAFAVTGCAMFCAGNARAADPVSFQLGIATEYLG
                     KGIGKSNGEPSLSGSVELSHNGFYGSVFAATAELSQGADAEIVTTGGYRTTLAGYGLD
                     LAVVNRDLPGTRAGVDANYIEYQADVSRKIGAVSTRLRVNYTADGFAATQEAWWLELQ
                     LGVALDASTKATVAVADRTAEGGAEYTAWNAGIKRKLPHDLALDLRWYDTDGHRFGPA
                     YDGRLVAALTYSF"
     gene            complement(1343..1792)
                     /locus_tag="VF633_03595"
     CDS             complement(1343..1792)
                     /locus_tag="VF633_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470174.1"
                     /translation="MPGIRILAVDDSESIQALLRQLLGTAGHEVVTADSPRQALRVLR
                     IFDPHIILTDFNMPGIDGHAFVRLLRRSPRFDSTPIFVLSSETETLKRDLMNDAGANG
                     WFSKPVCTDQLIAGVQAAARAVVRQHTASRPRFEPASPARHLTMPQR"
     gene            1906..3429
                     /locus_tag="VF633_03600"
     CDS             1906..3429
                     /locus_tag="VF633_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470175.1"
                     /translation="MASQYQTTSPDLRSIASVSQKAALLFGAFSDAAVAWASRPWNGY
                     ALAIASVAVALAARLAFGTLGEFFYLTLIPAVALPAILASRRAAVVAIILSVLANVWL
                     PPHDAVSDTVIAAMLFIAVGLGIGECGRARHRARARADKLASSLTTREAIIHAMLALT
                     PVVTLDENSTILTMSQPACTLFKVQEAEVTGQPFRRLVDAFDPAVIQASGKGGPGPDQ
                     YWLGRRSDGDVFALGIQLAVIAAPPKAPDVVLTLTDLSLWHSAETRNKELGDQLNKVW
                     RLNSLGEIAATLSHELNQPLTAAVSYLHASQADLARAGIFGASASRVIDLAKGQILRA
                     GDIIRRARALLSVGSLPLQAERVSSMVDDLGPILQLLSRAVDAQVQNEIHDTDDIVLA
                     DRIQFQQAMLNLVRNAVEAVSDQPRREIVIRGRAISPNAYRISVEDSGPGVARECIDR
                     MFQPLMTTKADGMGLGLSVTRTIIEKHGGALAYQTSDLGGAAFIFDLQRCLDPGDMK"
     gene            3426..4028
                     /locus_tag="VF633_03605"
     CDS             3426..4028
                     /locus_tag="VF633_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470176.1"
                     /translation="MTPSIFVIEDDPSVRDSLVTLLRSEGLRTRGFSSGTEFFSNLPT
                     DDFACVITDLRMPGVDGIELVDRLVDLRGEAWPVIVITGHGDVASAVSLMKAGVVDFI
                     QKPFEPHRLVETIRGCISRLQRVGKDMDRLALIDRRLALLTPRERQVFDLLVNGLSNK
                     EIATELQISSRTVEVFRAKVMSKMEAATLSALVRMGLRLE"
     gene            complement(4605..4689)
                     /locus_tag="VF633_03610"
     tRNA            complement(4605..4689)
                     /locus_tag="VF633_03610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4653..4655),aa:Leu,seq:caa)
     gene            4831..5781
                     /locus_tag="VF633_03615"
     CDS             4831..5781
                     /locus_tag="VF633_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HEX8470177.1"
                     /translation="MSDQALPDLAIVYEHPEWFEPLFAALDRHGVSYVKVPLAGHIFD
                     PTASPAPAKVVFSRVAMSSFLRDPEHPIFYAQSLFAHWEGQGTRVVNASALNIDTSKA
                     RQMSLIAKLGLKGPATRVAHRQADLPQAAEGLRFPVLVKADIGGAGAGITRYETPEAL
                     AEAAGEKWAPVGVNGISLIQEYAPRRGGKITRAETLNGKFLYALDIESPGDAFDLCPA
                     DACLVRPGAPTLTMTRTTPSAELIDAVERLAVAAELEVGGIEYLIDDRDGSVLFYDIN
                     GLSNFVAKPMDVLGFDPHDDLVEWLVGIVAEQSLKAEKAA"
     gene            5778..6821
                     /locus_tag="VF633_03620"
     CDS             5778..6821
                     /locus_tag="VF633_03620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8470178.1"
                     /translation="MRYGYWAPVFGGWLRNIEGEPRASWEHLSSIVKRSEEIGYDLTL
                     VAELNLNDIQGIEAPALDAWSSTAALAAVTSKIELMTAVRPNFHQPALFAKQAANIDQ
                     ISGGRLALNVVSSWWAKEAESYGLQFDEHDDRYARTTEWLQVLDRLWSDPRTDFSGSY
                     YELKDAIVEPKPVSTPARRRPVIYAGGESEAAKTLIARHCDAYVMHGDTPEVIAEKIA
                     DMKSRRESFGLPPMQFGMAGYAIVRDTEAEAEAELSRITTIPGLAEGRPPAGFANFDQ
                     WLSGTQLERELKIREYSVSNRGLRPGFVGTPETVRDRIEEFEAAGLNLVLLQMSPQAE
                     EMERFSAQVIRPN"
     gene            complement(6830..7645)
                     /locus_tag="VF633_03625"
     CDS             complement(6830..7645)
                     /locus_tag="VF633_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470179.1"
                     /translation="MKRFTVVGLLLAAALPAMAQDAAEPDWDISRDPVKKSVIAYIPL
                     TTGLALAVRCVDGALDSVIAGLPTYPRGRPTRPLHIAFGDDPLRETNWNVTTERTVAI
                     ADYPSAFARSLRKGGPLKIMIPRGADDGRNLVHSLTLPPSSAAIAETMSACNVPLEDP
                     RDALLPDIEERGLPAGITWARAPRPQYPNTNYATGYAVLTCVADAQGALNQCQIESEH
                     PAKSRFGEASLRAMPRARISSTTEPTGQITPRMIAFRVNFYMPGYQPPGTPRS"
BASE COUNT         1424 a         2410 c         2486 g         1509 t
ORIGIN      
        1 aggcttgtct cactcttgct tttcatcgcc tcgatggagt cttccagcgg cttgatcatc
       61 tcgtcagcga taccgtctgg agccaccaga gcctgggcct gagcgcgctg ggacgggtct
      121 cgcatcatgg cgacaccggg agtgaccacc ttggcgaacc aggcgtcgcc agctgcttcg
      181 gccgcgccgg catctgcatc acggtcggca tcaaggaggc cgcgcgtttc agccagtgca
      241 gccttgaaag ccgtacggtg ggcttcgagc cgttcgaggt agtagggatt gttcgagagc
      301 aggaagccgc gcattgcatt ttcctgtttc gagacccgga actcagcaat gctgatcagc
      361 tggttgacct tgttctccat gcgtcgcgtt tcgccgatgc gtgagatgtg cataaggttc
      421 atgccgacga cgcctcccat cacaaggatg gccgtcagca caacggcgaa cgccgcgaac
      481 attttccgac cgacgggcag gttgttgaag aaattcattg gcatatcctg gcgatgggga
      541 gggtcagtca gaagctgtag gtgagggccg cgaccaggcg gccgtcgtag gcggggccga
      601 aacgatgtcc atcggtgtca taccaacgca aatcgagcgc cagatcgtga ggcagcttgc
      661 gcttgatgcc cgcgttccag gcggtgtact cggcgccgcc ttcggctgta cggtctgcaa
      721 ccgccacagt ggccttggtg cttgcgtcaa gcgcgactcc caactgaagt tccagccacc
      781 aggcttcctg cgtcgcggcg aaaccatcgg cggtgtagtt cacgcgtaaa cgggtcgata
      841 ctgcaccgat cttgcgcgac acgtcggcct gatactcgat gtagttggcg tcaacgcctg
      901 cccgtgttcc cggcagatcc cggttgacga ccgcaaggtc cagtccgtat ccggcaagcg
      961 ttgtacgata gccgcccgta gtcacgatct cggcgtccgc gccctgggac agctcggcgg
     1021 tcgctgcgaa gacactgccg tagaagccgt tatgtgagag ctcgacagag ccggagaggc
     1081 tcggctcgcc gttgctcttg ccgatcccct tgcccaagta ttcagttgcg atcccgagtt
     1141 ggaaagagac tggatcggcc gcacgggcgt tcccggcgca gaacatcgcg cagccagtca
     1201 cagcaaatgc agtcacgatc ggaaggataa cagtgttatt catggtttcg atttcatggc
     1261 agccattctg gcgcccgctt tcgttgttaa ggttgaccta aataccgata ttctttgtaa
     1321 atcagcgtct acgtaagaat acctagcgct gtggcatggt gagatgccgc gcaggactgg
     1381 ctggctcgaa acggggcctg gatgccgtgt gctgcctgac caccgctcgg gccgcggcct
     1441 gaactccggc gatcagttga tcagtgcaga ccggcttgct gaaccatccg ttcgcacccg
     1501 cgtcattcat caggtcccgt ttgagggtct ccgtctcgct ggacaggacg aagatcggag
     1561 tgctgtcgaa gcgcggtgag cggcgcagca gacgcacgaa tgcatgaccg tcgatcccgg
     1621 gcatgttgaa gtccgtcaga atgatgtgtg ggtcgaaaat ccgtaggacc cgcaatgctt
     1681 gccggggact atctgcggtt acgacttcgt gtcctgcagt acccagtaat tgtcgcagca
     1741 acgcctgtat cgactcggag tcgtcgaccg ccaatattcg gatgccgggc attaagcctc
     1801 ctcagttcat ttgaggaggc taagggcaaa ttgcgtacag gttataacct aagttgtacg
     1861 tcgaataccc taggtattta gccttactta gcgtacaggt tgagtgtggc gtctcagtat
     1921 caaactactt ccccagatct ccgcagtatt gcgagcgttt ctcaaaaagc ggcgctcttg
     1981 ttcggggctt tctctgacgc ggcggtggcc tgggcctcgc ggccctggaa tggctacgcc
     2041 ctcgctatcg cttctgtcgc cgttgcgctc gcggcgcggc tggcgttcgg gacgctcgga
     2101 gagttcttct atctgacgct gattcccgcc gtcgcgcttc ctgcgatcct ggcgtcgcgc
     2161 cgcgcagctg tggtcgccat catcctttcg gtactagcca acgtctggtt gcctcctcac
     2221 gacgccgtca gcgacacggt tatcgccgcc atgctcttta tcgctgtcgg attgggcatc
     2281 ggggagtgcg gtcgggcccg ccacagggca agagccagag ctgacaagtt ggcgtccagc
     2341 ctgaccacac gcgaggcgat catacacgcg atgctcgcat tgacgcctgt cgtgacgctc
     2401 gatgagaact cgacgatcct gaccatgagc cagcccgcct gcacattgtt caaggttcag
     2461 gaggcggagg tgacgggtca gccgttccgg cgtcttgtgg atgcctttga cccagcggtg
     2521 atccaggcct cgggaaaggg cggcccgggg ccggatcagt actggctcgg tcggcgcagc
     2581 gatggcgatg tttttgccct gggtatccag ttggccgtta tcgcagcccc ccccaaggcg
     2641 ccggatgttg ttctgaccct gacagatctg agcctgtggc actccgcaga gacgcgaaac
     2701 aaggagttgg gcgatcaact caacaaggtc tggcggctga actcgctcgg cgagatcgcc
     2761 gccaccctgt cgcacgaatt gaatcaaccg ctaactgcgg cggtcagtta tctacacgcc
     2821 agccaagcgg atctcgcgcg ggccgggata ttcggcgcca gtgcgagccg cgtcatcgat
     2881 ctagccaaag gccagatatt gagggcaggg gacattatcc gcagggcgcg ggcgcttttg
     2941 tcagtcggct cgctgcccct gcaggccgag cgggtttcct cgatggttga tgaccttggt
     3001 cccattctcc agctcctgag ccgggcggtg gacgcccagg tgcagaatga aattcacgac
     3061 acggacgaca tcgtcctggc tgaccgcatt caattccagc aggcgatgct gaacctcgtc
     3121 cgcaacgcgg tggaggcggt atccgaccaa cctcgtcgag agatcgtcat ccgcggtcgc
     3181 gccatctcgc ccaatgcata tcgcatcagc gtcgaggaca gcggtcccgg cgtcgcacga
     3241 gaatgtatcg atcggatgtt ccagccgttg atgacgacca aagctgacgg catgggtctg
     3301 ggcctgtccg tgacccgcac catcatcgag aaacatggcg gcgctctggc gtatcaaacc
     3361 agcgacctcg gtggcgcggc ttttatcttc gatctgcaac gctgccttga tccgggagac
     3421 atgaaatgac cccctcgatc ttcgtcatag aagatgatcc gtccgtccgc gactccctcg
     3481 ttaccctgct gcgctccgaa ggcttgcgga cgcggggttt ctcgagcggc accgagttct
     3541 tctccaatct gccgaccgac gacttcgcct gcgtgatcac tgatttgcga atgcctggcg
     3601 tcgatggaat cgaactggtc gaccgtctgg tagacctgcg cggcgaagca tggccggtca
     3661 tcgtcataac cggccatggg gacgtcgcct cggccgtttc cctgatgaag gcaggggtcg
     3721 tggatttcat ccagaaaccc ttcgaacctc atcgtttggt cgaaacgatc cgaggatgca
     3781 tctctcgcct ccagcgcgtc ggtaaggata tggacagact ggcgctgatt gacaggcgac
     3841 tggcgcttct gacgcctcgc gaacggcagg ttttcgacct gcttgtcaac ggactgtcca
     3901 ataaggagat cgccacggaa ttgcagatca gttcacggac ggtcgaagtt tttcgggcca
     3961 aggtcatgtc caagatggag gcggcgaccc tctcggccct tgtgaggatg ggcctccggc
     4021 tggagtgaga tgtgatgcgt gggcgagacg ttgcgttcgg ttcttcagta ccggcgtcgc
     4081 cgcactaaca cgaggtcggc tagggtcgat gcgagccgtg gacaggcggc agcacctcga
     4141 ccggttctcc gcagcctccg caccgtccgc tcgattcccg gggcctcgcc aaggcaatgc
     4201 gagggcgtgc caccccctgc cattcctttc gggcctcgcc ccactgattc cacgaaaccc
     4261 tgagctcggt gacggcgccg catccgctac agcgggcggg ataaatgtcg gtctgccagg
     4321 tcaccgcgcg gataaatgca acgaacgcac tggcgtcaag cctgacttgc aaggtttctg
     4381 ccgccggcag gcgcagccca ggctgcagag gcgccgattc ggaaatctcc aaggacgact
     4441 gcatgtcaga cgaccggtcg ggatctggcg cgcgatgacg gccacgcccc cagaccagtc
     4501 atccaaagac ctatcgccgt cgcggagctg gagcggcctg taaccgtctg cacggtggct
     4561 gcgaccggga tagcctttct gctgttcagc gaagtgatcg ctgatggtgc gggcggccgg
     4621 ggtcgaaccg gcactcctct cggaactgga ttttgaatcc agcgcgtcta ccaattccac
     4681 cacgcccgca tgcagtcgcc cgtccggcgg gtggtccggg gcgggggctg atcctctagc
     4741 gcaaccaggc ggtattgcgc cagtcccgtg cggttgagaa tctactccac gggcttgcgt
     4801 ttcatcgagg tgtaactcat ctgccttggc atgagcgacc aggccctccc cgatctcgcg
     4861 atcgtctatg aacaccccga gtggttcgaa ccgttgtttg cagcgctgga ccggcatggc
     4921 gtcagctatg tgaaggtccc gctggcgggt cacatcttcg acccgacggc gtcgcctgct
     4981 ccggctaagg tggtgttcag ccgggtggcg atgtcgtcgt tcctgcgcga tccggaacat
     5041 ccgatcttct acgcccagag cctgttcgcg cactgggagg gacaggggac gcgggtggtc
     5101 aacgcctcgg ccctgaacat cgacacctcc aaggcgcggc agatgtcatt gatcgccaaa
     5161 ctcggtctga aaggtcccgc gacccgcgtc gctcaccgtc aggccgacct gccgcaagcc
     5221 gccgaggggc tgcgctttcc ggtgctggta aaggccgaca tcggcggcgc cggggcgggc
     5281 atcacccgct atgaaacccc tgaagcactg gccgaggccg ccggtgagaa atgggccccg
     5341 gtcggcgtca acggcatctc tttgattcag gaatacgcac cgcgccgggg cggcaagatc
     5401 acccgcgccg aaacgctgaa cggcaagttc ctgtacgccc tcgacatcga gagccccggc
     5461 gacgccttcg acctgtgccc cgccgacgcc tgtctggtgc ggcccggcgc gccgacgctg
     5521 accatgactc gcaccacacc gtccgccgag ttgatcgacg cggtcgagcg ccttgcggtc
     5581 gcggcggaac tggaagtcgg ggggatcgag tatctgatcg acgaccgcga cggctcggtg
     5641 ctgttctacg acatcaatgg cctgtcgaac ttcgtcgcca agccaatgga tgtgctgggc
     5701 ttcgaccccc acgatgatct ggtcgaatgg ctggtcggca tcgtcgccga acagtctctc
     5761 aaggcggaga aggcggcatg aggtacggtt actgggctcc cgtcttcggc ggctggctaa
     5821 ggaacatcga gggcgaaccg cgcgcctcgt gggaacatct gtcgtcgatc gtgaaacggt
     5881 ccgaggagat cggatacgac ctgacgctgg tcgccgagct caacctcaac gacattcagg
     5941 gcatcgaagc cccggcgctg gatgcctggt cgagtacggc ggcgctggcg gcggtcacct
     6001 cgaagatcga gctgatgacg gcggtgcgtc cgaacttcca ccagcccgcc ctgttcgcca
     6061 aacaggccgc caacatcgac cagatctcgg gcgggcggct ggcgttgaac gtggtctcct
     6121 catggtgggc caaggaggcc gagagctacg gcctgcagtt cgacgagcac gacgaccgct
     6181 atgcgcggac caccgaatgg ctgcaggtgc tggacaggct gtggtccgat ccgcgtacgg
     6241 atttctcggg cagctactac gagctgaagg acgccatcgt cgaacccaag ccggtctcaa
     6301 cgcccgcccg ccgccgtcca gtcatctatg cgggcggtga atcagaggcc gccaagaccc
     6361 tgatcgcgcg ccactgcgac gcctatgtca tgcacggcga cacgcccgag gtcatcgcgg
     6421 agaagatcgc cgacatgaag tcgcgccgtg aaagcttcgg cctgccgccg atgcagttcg
     6481 gaatggccgg atacgccatc gtccgcgata ccgaagctga agccgaggct gaactcagcc
     6541 gtatcaccac cattcccggt ctcgccgaag gcaggccgcc cgccggcttc gccaatttcg
     6601 atcagtggct gtcgggcacc cagctggagc gcgagctcaa gatccgcgaa tactcggtct
     6661 cgaaccgcgg cctgcgcccc ggcttcgtgg gcacgccgga gaccgtccgc gaccggatcg
     6721 aggagttcga agctgcgggt ctcaacctcg tgctgctgca gatgtcgccg caggccgagg
     6781 agatggagcg gttctccgcc caggtcatcc gcccgaactg agaagacgat caggagcgtg
     6841 gcgttccggg cggttgatag cccggcatgt agaagttgac gcggaaggcg atcatccggg
     6901 gcgtgatctg accggtgggc tcggttgtgc tagagatgcg tgctctcggc attgccctga
     6961 gggaagcctc gccgaagcgg cttttggcgg gatgttcgga ctcgatctga cactggttca
     7021 gcgcgccctg ggcgtcggcg acacaggtca ggaccgcgta gccggtcgcg tagttggtgt
     7081 tcggatactg agggcgaggc gctctggccc atgtgatgcc cgcaggcagg ccccgttcct
     7141 cgatgtccgg cagtagagcg tcgcgcgggt cctccagtgg gacattacag gccgacatgg
     7201 tctcggcgat ggcggcgctg gaaggtggca gagtcaggct atgaaccaga ttgcggccgt
     7261 catcggcgcc ccgggggatc atgatcttga gcggaccacc cttgcgcagg ctgcgagcga
     7321 aagcggacgg gtagtcagcg atggcgacag tgcgttcggt ggtgacgttc caattcgtct
     7381 cgcgcagggg atcgtcgccg aaagcgatat gcaggggcct tgtcggtcgc cctcgtgggt
     7441 aggttggaag cccggcgatg acggaatcca gcgctccgtc gacgcaccgc accgccagcg
     7501 ccaaacctgt cgtgagcggg atataggcga tgacggactt ctttacgggg tcccgtgaga
     7561 tgtcccagtc cggctctgcg gcgtcctgcg ccatagcggg aagagccgct gcgagcaaca
     7621 gcccgacgac ggtgaagcgc ttcatgacaa ctcccgagac agttaggagc aagctggcac
     7681 acgtcagggt aggggatcaa ccgccgattc atgaagcctt ggctcaggca gtcgcttcca
     7741 catgactgtc ggtgccgccg tgctggacgc ggcgagcccg gatgtacatc tcgacgcgct
     7801 gcaggacgat catccctacc acgaacacc
//
LOCUS       DASVTM010000128         4874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_597806,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000128 DASVTM010000000
VERSION     DASVTM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597806"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VF633_03630"
     CDS             complement(<1..804)
                     /locus_tag="VF633_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8470180.1"
                     /translation="MSDTILITGGAGFVGRHVAKALLEQGHKIRVLDSLIEQVHPTRT
                     RPDELDPEVELLVGDIRDEAMVLRAIAGVDKVIHLAAEVGVGQSMYAVDRYTSVNDHG
                     TAVLFQQLIERPVQRVVVASSMSIYGEGLYRTQDGELMSDVVRGPRNPDGTWDPLDAL
                     GRPMIPSPTPETKQPALKSVYAINKFVQERLTLTLTAQYGMVGTALRLWNIYGPGQAL
                     SNPYTGVLAIFASRIANGQSPMVFEDGQQRRDFVHVSDVARAFLLALDKP"
     gene            1112..2215
                     /locus_tag="VF633_03635"
     CDS             1112..2215
                     /locus_tag="VF633_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX8470181.1"
                     /translation="MARDKIRVGVVGVGNCASSFVQGLTHYREALANEPPPGLMNVEL
                     GGYHVGDMEISAAFDIHAGKVGRDVSQAIFSRPNNTVQFATPPNSGVQVMRGPTLDGL
                     GKYLVGDIEESSDEPVDVAEVLRRSGTDVLVSYLPVGSQRATEFYAEQALSAGCAFVN
                     CIPVFIASDPAWAKRFEQRGLPIVGDDIKSQVGATIVHRMLANLMRERGVRLDRTYQL
                     NFGGNTDFKNMLERERLESKKLSKTRAVTSQLDVPLDPDDVHVGPSDFVPWLTDRKWA
                     HIRLEGTTFGDVPLSIELKLEVWDSPNSAGVVIDAVRCAKLALDRGVSGALIGPSSYF
                     MKSPPQQFTDHEAHERTLRFIAGSDHVKVRLDS"
     gene            2212..2805
                     /locus_tag="VF633_03640"
     CDS             2212..2805
                     /locus_tag="VF633_03640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8470182.1"
                     /translation="MSASTVYLVRHGAHDRLGRVLCGRDDTVLNEEGRRLAGRLGEHF
                     ARLGIDAVQTSPLSRATETAEPIARLAGRPAAIDQALSEIDFGAWTGSTFDALEGRPD
                     WRLWNEDRGRRRPPGGETMLEAQARIAGWLEGLRTGAKALVAVSHADLIKAAVCYVLG
                     LPLHLYDRFEIDPGSITTLTVADWGMKVQAMNERALA"
     gene            2807..3574
                     /locus_tag="VF633_03645"
     CDS             2807..3574
                     /locus_tag="VF633_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HEX8470183.1"
                     /translation="MDTEQIRARVTALGDWFHNLDLKGVQTAPDHFLADYPRNKFSRF
                     AGAIPADLTGKTVLDIGCNAGFYSMEMKRRGAARVVGLDSDDRYLAQARLAAEVLEVD
                     IEFRNLSVYDLAALGERFDLVIFMGVLYHLRHPLLALDLIHEHVAGDLLLFQTMQRGS
                     NDVDPVQADYDFFQQSHFDSPGYPKMHFIEHEYAGDWTNWWAPNAACSQAMLRSAGFA
                     IVEQPESEVYLCRRVDRPKDAGAVYPAKPQRNGSAQG"
     gene            3571..4503
                     /locus_tag="VF633_03650"
     CDS             3571..4503
                     /locus_tag="VF633_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEX8470184.1"
                     /translation="MIEAIKIWNEPNNKSHWDPNLDPEWDLFAEMTRLTGQAVRAENS
                     NLTRVLGGMSPIDPAFIRRLEARGVMDHIDAVAVHGFPLDWNLWPIDAWPSKIEEIRA
                     VTNKPVWVTEVGVSSFGSEEVQAWGVRKTADLLRGKAPRIHWYSLYDLPQEWEATTRH
                     KEAEGSSYYRHFHMGLLRQDGTPKPAAEAFAEHARGMGICQWFHFEDHRLDDAVAWLR
                     RLGVRQLRTGLSWADSFRPDALEWFDRQMDALAEFDVTVTFCFTPEHRGLEPHHSSPP
                     QVAAEFAEFCASMVRRYAPTSAAKSLAENADKAA"
     gene            complement(4519..>4874)
                     /locus_tag="VF633_03655"
     CDS             complement(4519..>4874)
                     /locus_tag="VF633_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470185.1"
                     /translation="WLRSADGGLLKADALDHDVEHDLIGPQDLAWDVAGAAVELGFSA
                     TALAAQTALISGRTIDPDLLVFLTPCYMAFRLGAARMAQRMQDETEGRLNAQAAERYA
                     RGLTDWLDEDHSITG"
BASE COUNT          906 a         1519 c         1570 g          879 t
ORIGIN      
        1 aggcttgtcg agagccagaa ggaaggcccg tgcgacgtcc gacacgtgca cgaagtcacg
       61 ccgttgctgt ccgtcctcga acaccatcgg cgactgaccg ttggcgatcc gcgaggcgaa
      121 gatcgcgagt acgccggtgt aggggttcga cagggcttgc cccggcccgt agatgttcca
      181 gagccgcagc gcggttccca ccatgccgta ttgggcggtc agggtcaggg tcagccgttc
      241 ctgaacgaac ttgttgatgg cgtagacgct tttcagcgcc ggctgtttcg tctccggcgt
      301 cggggagggg atcatggggc ggccgagggc gtccaagggg tcccatgttc cgtccggatt
      361 gcgcggcccg cgcaccacgt cagacatcag ctcgccgtcc tgagtgcggt agagtccttc
      421 cccgtagatg ctcatggacg acgcaactac gacgcgctga acgggccgct cgatcagttg
      481 ctggaacaac accgccgtcc cgtgatcgtt gaccgaggtg tagcgatcga ccgcgtacat
      541 gctctggccg acaccgactt cggcggccag gtggatgacc ttatcgacgc ctgcgatggc
      601 tcgcagcacc atggcttcgt cacggatatc tccgaccaga agttcgactt ccgggtccaa
      661 ctcatccggg cgggtgcggg tcgggtgaac ctgctcgatg aggctgtcga ggacccgaat
      721 tttatggcct tgctccaaca gcgccttggc cacgtgccgt ccgacgaagc cggcgcctcc
      781 cgtgatcaga atggtgtcgc tcacgacaaa tcccctcatg cttgaacggt cggcgcaggg
      841 cgccagcgac cttctgcttc agcgtcgccg taccgaggaa catcatcatc aaccgatcgg
      901 ttccgtgaaa cctgtcaaaa gcgaaacgaa tactatgtct gattcccgac atatcaagtc
      961 agtactgatg tcgagtacct gacactaagc accgacagca acgtgtggta gcgacgtcaa
     1021 ggatcaatgg aaccaacagc tttgtagttg cttatagcca agccagaccg atcccgaagg
     1081 ggctcacggt tgtcagccca ggagtatgaa aatggcgcga gacaagattc gggtgggagt
     1141 ggtcggtgta ggcaactgcg cctcttcctt cgtccagggc ctgacccact atcgtgaggc
     1201 tctggcgaac gagccgcctc cgggcctgat gaacgtggag ctcggcggct atcatgttgg
     1261 cgatatggag atttcggctg ccttcgacat ccacgccgga aaggtggggc gcgacgtctc
     1321 tcaggcgatc tttagtcggc ccaacaacac ggtgcagttc gccacgccgc cgaacagcgg
     1381 cgtccaggtg atgagagggc cgacgctcga cgggcttggg aagtacctcg tcggggatat
     1441 cgaagagtcg agcgacgagc ctgtcgacgt cgcggaggtc ttgcgccgat cgggcaccga
     1501 cgtgcttgtg tcctacctgc cggtcggctc ccagcgcgca accgagttct acgctgagca
     1561 ggcgctgtcg gcgggctgcg ccttcgtcaa ctgcatcccg gttttcatcg cttccgatcc
     1621 agcctgggcc aagcggttcg agcaacgggg cctgccgatc gtaggcgacg acatcaagag
     1681 ccaggtcggc gcgaccatcg tccatcgtat gctcgccaat ctgatgcgcg aacggggcgt
     1741 gcggctggat cggacctatc agctcaattt cggggggaac accgacttca agaacatgct
     1801 ggagcgtgag cggctggagt ccaagaagct ctcgaaaacg cgggcggtga ccagccaact
     1861 ggatgtgccg ctcgacccgg atgacgtgca cgttggtccg agcgactttg taccgtggct
     1921 gaccgatcgc aaatgggcgc acattcgcct ggaaggcacg acgttcggtg acgtgccgct
     1981 gagcatcgag ttgaagctgg aggtgtggga cagccctaac agcgcgggtg ttgttatcga
     2041 tgccgtacgg tgcgccaaac tggcgctgga ccgtggcgtg tccggcgcgc tgatcggacc
     2101 cagcagctat ttcatgaagt ctccgcccca acagttcact gatcatgagg cgcacgaacg
     2161 cacgctgcgt ttcatcgccg gctcggacca cgtcaaggtt cgtctcgact cgtgagcgcc
     2221 agcactgtgt atctggttcg gcatggcgcg catgatcgtc tggggcgagt tctgtgcggg
     2281 cgcgacgaca ccgtcctgaa cgaggaaggt cggcgtctcg ccgggaggct cggcgagcat
     2341 ttcgcccgtc tcggcattga cgcggttcag accagtccgc tttctcgcgc gaccgaaacc
     2401 gcagagccga tcgcccgact ggccggccga cctgcagcca tcgaccaagc gctgagcgaa
     2461 atcgacttcg gcgcatggac cggctcaacg ttcgatgccc tcgaaggccg cccggactgg
     2521 cgcctttgga acgaggatcg aggccggcga cggccgccgg gcggcgagac gatgctggaa
     2581 gcacaggcac ggatcgccgg ctggcttgaa gggctcagga ccggggcgaa ggcgctagtc
     2641 gccgtcagcc acgcggacct catcaaagcc gcggtctgct acgtgctcgg actgccgctt
     2701 cacctgtacg accggttcga gatcgatccg ggctcaatca ccacactcac cgtcgccgat
     2761 tggggcatga aggtgcaggc catgaacgag agggcccttg cctgacatgg acacagaaca
     2821 aatccgcgct cgggtcaccg cactgggcga ctggttccac aatctcgacc tgaagggggt
     2881 tcagacagct cctgaccact tcctggcgga ttatccccgg aacaagttca gccggttcgc
     2941 cggtgccata cccgcagacc tgaccggcaa aaccgttctg gatatcggct gcaacgccgg
     3001 cttctactcg atggagatga agcgacgcgg agcggcgcgg gtggtcgggc tggactcgga
     3061 tgaccgatat ctggctcagg cccgtctcgc cgccgaggtg ctggaggtcg acatcgagtt
     3121 ccgcaacctg tccgtctacg atctggccgc gttgggagag cggttcgacc tagtgatctt
     3181 catgggcgtg ctctatcacc tgagacatcc cctgctggct ctggacctga tccacgagca
     3241 tgtggcgggc gacctgctgt tgtttcagac gatgcagcgg ggatcgaacg atgtagatcc
     3301 ggtgcaggcc gactacgact tcttccagca gtcgcatttc gacagtcccg gctacccgaa
     3361 aatgcacttc atcgagcacg agtacgccgg tgactggacc aactggtggg cgccgaacgc
     3421 cgcctgttcc caggccatgc tgcgcagcgc cggcttcgcc atcgtcgagc agccggagag
     3481 cgaagtgtat ctgtgccgcc gagtcgaccg gcccaaggac gctggcgcgg tctatccagc
     3541 caaaccccaa cgcaacggaa gcgctcaagg atgatcgagg cgatcaagat ctggaacgaa
     3601 cccaacaaca agtcgcactg ggatccgaac ctcgatcccg aatgggacct gttcgccgag
     3661 atgacccggc tgaccggaca ggccgtgcga gcggagaaca gcaacctgac acgggtgctg
     3721 ggcggcatgt ccccgatcga tcctgccttc atccgccgcc tggaggcgcg cggggtcatg
     3781 gatcacatcg acgcagtcgc cgtgcacggc ttcccgctgg actggaacct gtggccgatc
     3841 gacgcctggc cctcgaagat cgaggaaatc cgcgccgtca cgaacaagcc ggtctgggtg
     3901 accgaggtgg gggtctcgtc gttcggctcc gaggaggtgc aggcgtgggg cgtgcgcaag
     3961 acggccgatc tgctgcgcgg caaggcgcca cgcattcatt ggtacagtct ctacgacctg
     4021 ccacaggaat gggaggcgac cacccggcac aaggaggccg aagggtcgtc ctactaccgg
     4081 catttccata tggggctgct gcgtcaggac gggacgccca agcccgccgc agaagcgttc
     4141 gccgagcatg cccgaggtat gggcatttgc cagtggttcc acttcgagga tcatcggctc
     4201 gatgacgccg tcgcctggct ccgccggctg ggagtgcgcc aattgcggac cgggttgtcg
     4261 tgggccgaca gcttcaggcc tgacgcgctg gagtggttcg atcgtcaaat ggacgcgttg
     4321 gccgagttcg acgtcactgt caccttctgc ttcacgcccg agcatcgcgg gctggaaccc
     4381 caccacagca gcccgccaca ggtcgcggcc gagttcgccg agttctgcgc ctcgatggtg
     4441 cgccgttacg cgcccacatc ggcagccaag tcgctcgccg agaacgctga caaggccgca
     4501 tgattggcgg cggcgcggct atccggtaat gctgtggtct tcgtccagcc agtccgtcag
     4561 tcctctggcg tagcgttcgg cggcctgggc gtttaagcga ccctccgtct catcctgcat
     4621 gcgctgggcc atacgcgccg cgcccagtcg gaaggccatg taacagggtg tcaggaagac
     4681 gagcagatcc gggtcgatcg ttcgacccga aatcagagcg gtctgggcgg ccagcgctgt
     4741 ggctgaaaac ccgagttcaa ccgctgctcc ggcgacgtcc caggcgaggt cctgcggtcc
     4801 gataaggtcg tgctccacgt catgatcgag cgcatcagcc tttagcagcc cgccgtcggc
     4861 cgagcgcagc catt
//
LOCUS       DASVTM010000129         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_13265, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000129 DASVTM010000000
VERSION     DASVTM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13265"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VF633_03660"
     CDS             complement(<1..151)
                     /locus_tag="VF633_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470186.1"
                     /translation="MARAQFQKGQKVWVECVGAWAHIEKVQPVWAKGFDEPVRVTYDV
                     GLGREF"
     gene            complement(253..666)
                     /locus_tag="VF633_03665"
     CDS             complement(253..666)
                     /locus_tag="VF633_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8470187.1"
                     /translation="MKIRDVMSKDVQVARPGDTIQDVAARMAAGDFGFIPVADGDRLV
                     GTVTDRDIAVRAVAKGLSATTQVSDIMTAEAVTALDSADLKTALDAMGGGKIRRLPVV
                     DKHDRLVGVVSLGDLSTRVKEKYAGETLETISQPG"
     gene            774..2273
                     /locus_tag="VF633_03670"
     CDS             774..2273
                     /locus_tag="VF633_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8470188.1"
                     /translation="MTAAAEAKTILTRLGVADSVWNGDLPTRSPIDGSSAGSLHQATS
                     DEVEAAITGSAEAFKAWRRVPAPRRGELVRLLGEELRAAKTDLAMLVTLEAGKVVSEG
                     LGEVQEMIDICDFAVGLSRQLYGLTMPSERPGHHMRETWQPLGPVAVITAFNFPVAVW
                     AWNAALALVCGDPVIWKPSEKTPLTALGVQAVFERAVARFGDAPDGLSQLLIGGREIG
                     EALSADPRVPLISATGSTRMGKAVAQSVAGRLGRSLLELGGNNAMIVTPSADLEMAVR
                     AIAFSAVGTAGQRCTSLRRLFVHEDIAESLIARLAKAYATLPIGDPRTEGVLVGPLID
                     EAAAQGFDAALADAVAQGGEIVVGGTRVQNDGVYVTPAIVRMPGQSAVVQHETFAPIL
                     YVLTWSDFDEAVEMQNAVPQGLSSCVFTDSVREAEAFLSAWGSDCGIANVNIGPSGAE
                     IGGAFGGEKDTGGGRESGSDAWKAYMRRQTQTVNFSAELPLAQGVKFEV"
     gene            complement(2275..2724)
                     /locus_tag="VF633_03675"
     CDS             complement(2275..2724)
                     /locus_tag="VF633_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8470189.1"
                     /translation="MNFNSALILLAIIIAGGATALQAPTNARLAVAMASPVNAAFVSF
                     AVGTVALGLMAALLHTKPDLAAARALPGWAWMGGLYGCVFVITAAWAVPRLGAALTIT
                     LMVAGQLLLSLGLDHFGALGVPRHPIGIGRLAGVALVFIGVVMVRRF"
BASE COUNT          482 a          988 c          940 g          461 t
ORIGIN      
        1 gaaattcccg acccagaccg acgtcgtacg taacccgcac cggctcatcg aagcccttcg
       61 cccacacggg ctgaaccttt tcgatgtgag cccacgcgcc tacgcattcg acccagacct
      121 tctggccctt ttgaaattga gcgcgcgcca tccttgtcct tccagtatcc catggacacc
      181 ctgcagtatg ggcgattagc gacccgtgtc cgagcagggt gaaggaggga tgaagggaac
      241 ctgggctcgc tgtcagccag gctgggaaat ggtctccagg gtctcgccgg cgtacttttc
      301 cttcacccgc gtggacaggt cgccgagcga cacgacgccg accagacgat catgcttgtc
      361 cactacgggg aggcgacgga tcttgccgcc gcccatggcg tccagcgcgg tcttcaggtc
      421 ggcgctatca agtgctgtaa ccgcctctgc ggtcatgatg tcggacactt gagtcgtggc
      481 actcagtcct ttggccaccg cgcggacggc gatgtcgcga tcggtcacgg tgcctaccag
      541 ccggtccccg tcggcaacgg gaataaagcc gaaatcaccg gccgccatcc gggcggcaac
      601 gtcctggatc gtatctccgg ggcgagcgac ctgcacgtcc ttgctcatca cgtcacggat
      661 tttcattggg gaactcctgt tgtgtcggcg attgaacccc ggtcgcgtga cccggttccg
      721 ctttcgcgac actggcgagc gaggtaaggc catcgccccg acggagagac gccatgactg
      781 ccgctgccga agccaagacc atcctgaccc gcctgggagt cgccgacagc gtctggaacg
      841 gagaccttcc gacccgctcg ccgatcgacg gttcgagcgc cggatcgctg catcaggcga
      901 cgtctgatga ggtcgaggcc gcgatcaccg gctcagccga ggcgttcaag gcctggcgcc
      961 gtgtccccgc gccgcgccgt ggcgagttgg tgcggctgct gggtgaagaa ctgcgcgccg
     1021 ccaagaccga cctcgccatg ctggtcacgc tcgaagccgg caaggtcgtg tccgaaggcc
     1081 tcggcgaggt gcaggagatg atcgacatct gcgacttcgc cgtcggcctg tcgcgccaac
     1141 tctacggcct gaccatgccg agcgaacggc ccggccacca catgcgcgaa acctggcagc
     1201 cgctgggtcc ggtcgccgtg attaccgcct tcaacttccc cgtcgccgtc tgggcctgga
     1261 acgcggcgct ggcgctcgtc tgcggcgatc cggtgatttg gaaaccatcc gagaagacgc
     1321 cactgaccgc gctcggtgtg caggcggtgt tcgaacgcgc ggtcgcccgc ttcggcgatg
     1381 cgcccgacgg cctgagccaa ctgctcatcg gcggccgcga gatcggcgag gctctgtccg
     1441 ccgacccgcg cgtacccctg atctcggcca ccggctcgac ccgcatgggc aaggccgtgg
     1501 cgcagagcgt cgccggtcgg ttgggtcgct cgctgctgga actcggcggc aacaacgcca
     1561 tgatcgtcac cccgagcgct gatcttgaga tggccgtccg cgccatcgcc ttctcggccg
     1621 tgggcacagc cggccagcgc tgcacctcgc tgcgccgtct gttcgtgcat gaggacatcg
     1681 ccgagagcct gatcgcccgc ctcgccaagg cctatgcgac cctgccgatc ggcgaccccc
     1741 gcaccgaagg cgtgctggtc ggcccgctga tcgacgaggc ggcggcgcaa gggttcgacg
     1801 ccgccctggc cgatgccgtt gcgcagggtg gcgagatcgt cgtcggcggc acgcgcgtcc
     1861 agaatgacgg cgtctacgtc acccccgcca tcgtcaggat gccgggccag agcgcggtcg
     1921 tgcagcacga aaccttcgcc cccatccttt atgtcctgac gtggagcgat ttcgacgagg
     1981 cggtcgagat gcagaacgcc gtgccgcagg gcctgtcgtc gtgcgtcttc acagacagcg
     2041 tgcgcgaggc cgaggccttc ctgtcggcct ggggctcgga ttgcggcatc gccaacgtca
     2101 acattggccc gtccggcgcc gagattggcg gggcgttcgg cggcgagaaa gacaccggcg
     2161 gcgggcgcga gagcggctcg gacgcgtgga aggcctatat gcgtcgccag acccagacgg
     2221 tgaatttctc cgccgaactg ccgctggctc aaggcgtgaa gttcgaggtc tgaactagaa
     2281 gcggcggacc atcaccacgc cgatgaacac cagcgccacg cccgccagtc gcccgatccc
     2341 gatcgggtga cgcggcacgc ccagcgcgcc gaaatggtcg agcccgaggc tcagcagcaa
     2401 ctggcccgcg accatcaggg tgatggtcag ggcggcgccg agacgcggca cggcccatgc
     2461 cgctgtgatg acgaagacgc agccgtatag accacccatc caggcccagc cgggcagggc
     2521 tcttgctgcg gcaagatcag gcttggtgtg cagcagcgcc gccatcaggc ccagcgccac
     2581 cgtccccacg gcgaacgata cgaacgccgc gttgaccggc gacgccatgg ccacagccag
     2641 ccgcgcattg gtcggcgcct gcagggcggt cgcgcccccg gcgatgatga tggccagcag
     2701 gatcaaggcg gagttgaagt tcattttgtc ccgctaacac agggtcgcaa agaggggcag
     2761 ggggctttca ccagtcgtcc gtcctcgcca cgcccccgaa gatttcgccc agccgtgcgc
     2821 gggtcgatcg atgtgcatcc tcgggcaggg catccggcga gaaccatccg a
//
LOCUS       DASVTM010000130         6245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1021668,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000130 DASVTM010000000
VERSION     DASVTM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021668"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VF633_03680"
     CDS             <1..1791
                     /locus_tag="VF633_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470190.1"
                     /translation="QLDHGYIRFYAKYLDDRQPNYSLFPLIASGSNNDPSFSNLPGTD
                     GLKDTILSSNNAVYNGVDQNNNPTTFDRRNGVRGVVKAIGAEGQYDINGWTISEKLRY
                     SRISGELNDDISLVTMPAAVMAAAFGGPGARLSYATGPNAGQVVANPSTLNGNGLLQV
                     GVYVNAKLNNLDNVTNDLRASRVWNIGAGKLTTTAGFYASSQNIDMYWSFANALEDFA
                     SGGNGSRYNLTTATNIPVTDNGMIAYGFATATALYTYHQRYDVNYQTLAPYASANYQI
                     GKLAVGGSVRLDHGDVSGSLYGASLGGGRNGQATIDVNGDGVISLPETKVAVLPLAQP
                     GIVDYSYDYVSYSVGVNYRIAEPVSVFGRYSRGGRAAGERQLFQPQFNSTTGQPVDAD
                     TAYGEVEQAEAGVKFRRPGLVVYATGFWASTGENNTQIGADASGAPKVISFDRTYSAK
                     GIELESEYSYGPFSVALGATYAKASIDGDSANAALVGNRPRHQPELFYNVRPQFERGM
                     FTIGATINGTTSSYAQDDNVLVQPGYVIVNPYFFVRPLPRIELGLTAYNVFDEIAIVS
                     INSSAIPASGVVSAQTLNGRTISASLRYTF"
     gene            1948..2718
                     /locus_tag="VF633_03685"
     CDS             1948..2718
                     /locus_tag="VF633_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8470191.1"
                     /translation="MLIDPMFYAVAIPAVILLGLTKGGFAGIGVVAVPMMALVISPVQ
                     AAAIILPILLAQDVVGVWSFRKTWDKGLVILMLPSAAVGILVGWLLAARVEVAAVELA
                     IGLIAVTFSLQQLWAGRAVRAAEVIEAGPARRWLGVLCGLVSGFTSQVAHAGAPPFQI
                     YVLPRRLPRDMFIGTSAVFFAAVNWMKLPAYIALGQFTPQVLTTAAVLLPLALVSTVA
                     GVWLVRRVPAKGFYQVIYVVLAAVGVKLAWGGASGLLG"
     gene            complement(2884..3735)
                     /locus_tag="VF633_03690"
     CDS             complement(2884..3735)
                     /locus_tag="VF633_03690"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX8470192.1"
                     /translation="MTNSTYSFAGRTVNRLGYGAMQLAGPGVFGPPRDPDAALAVLRA
                     ARDAGVNHIDTSDFYGPHVTNQLIHKALHPYPDDLLIVTKVGAVRGPNAEWHRACSAD
                     DLTSAVHDNLRNLGLDVLDVVNFRAMFDVNGPAEGSIEEPLSALAELQNQGLIRHIGL
                     SNVTPAQYAQASHIVPIVGVQNQYNLARRDDDAFIDQLAAEGIAYVPFFPLGGFSPLQ
                     SEGLTKVAADLGVAAMQVALAWLLQRSPNILLIPGTSSTLHLAENLASSRLILPDAAI
                     QALEGLG"
     gene            3829..4737
                     /locus_tag="VF633_03695"
     CDS             3829..4737
                     /locus_tag="VF633_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8470193.1"
                     /translation="MPHDLADLNAFVAVARARGFRNAARIEGVSASALSDAVRRLETG
                     LGVRLLHRTTRSISLTEAGSRLIDRLEPALSEVDAALAGVGTLGDEPAGTLRLNVPVS
                     ATRLVLPHIVPAFLAAYPQIRMEVTAEDSFVDVLAAGADAGIRYDERLEQDMIAVPIG
                     PRVQRFACAAAPFYLDTHGRPGHPRDLLGHACLRGRFSSGVSPAWEFEQGGETVMIEG
                     DGPLLVNMGPAIDLAVEAAVAGAGVIYVFEDWIAQQLARGDLEPVLQDWWPSFSGPFL
                     YYSGRRLVPPPLRAFIDFIRDPRSSG"
     gene            complement(5073..5669)
                     /locus_tag="VF633_03700"
     CDS             complement(5073..5669)
                     /locus_tag="VF633_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470194.1"
                     /translation="MVEGGRNLNGDFYAFDGRDFRFHDPEADRRYRSFLDSLGVTAAA
                     GIPCPVLCTFGGNFHYLSRQEVWDGFRIPNSPLEGRYLSQAAFEAAVRAMISGALAFQ
                     ADLVGLGLTVSFALPPRRVPVTASRWAFLEIERAVPPMFAALGVGIIDLRSETLDREG
                     TLRSEFAHPDPADDTHGSVAFGAMVVARAIGARDVPTP"
     gene            complement(5749..>6245)
                     /locus_tag="VF633_03705"
     CDS             complement(5749..>6245)
                     /locus_tag="VF633_03705"
                     /inference="COORDINATES: protein motif:HMM:NF020459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HEX8470195.1"
                     /translation="GLEYPSCPGIIAGEYSQDRYVFHNQTMAEVMETMDECFHLLQSL
                     RPGLRILLTVSPVPLTATKTGDHVLVASTHSKSVLRAVAGELTAREMLYDYFPSYEII
                     TAPAFRGYFFAPNMREVSPRGVDFVMTHFLPHLLRVPVAAILPPASVDPVCDEMVLDR
                     YAAP"
BASE COUNT         1151 a         1968 c         1975 g         1151 t
ORIGIN      
        1 cagctcgacc acggctacat ccgtttctac gccaagtatc tggacgaccg ccaacccaac
       61 tacagcctgt tccccctgat cgctagcggc tccaacaacg atccgagctt ctcgaacctt
      121 cccggcaccg acggcctgaa ggacaccatt ctgtcgagca ataacgccgt ctacaacggg
      181 gtcgatcaga acaacaatcc gaccaccttc gatcgccgca acggcgttcg cggcgtggtc
      241 aaggcaatcg gcgccgaggg tcagtacgac atcaacggct ggactatctc agagaagctt
      301 cggtactccc ggatctcggg cgaattaaac gacgacatct ctctggtcac catgcctgct
      361 gcggtcatgg ccgcagcctt cggcggtccc ggcgctcgcc tgagctacgc tacgggcccc
      421 aacgccggtc aggttgtcgc caacccgtcg acactaaacg gcaacggtct gctgcaggtg
      481 ggcgtctatg ttaacgccaa gctcaataac ctggacaatg taaccaatga cctgcgcgcc
      541 agccgcgtct ggaacatcgg cgccggcaag ctgaccacga ctgctggttt ctatgcctct
      601 tcccagaata tcgacatgta ctggagcttc gccaatgctc tggaagactt cgccagtggt
      661 ggtaacgggt ctcgctacaa cctgacgacc gcgaccaaca ttccggtcac cgacaacggc
      721 atgatcgcct atggcttcgc gacggcgacc gcgctctaca cctatcacca gcgctacgac
      781 gtgaactacc agaccctggc gccctatgct tcggccaact accagatcgg caagctggcg
      841 gtcggcggca gcgtccgtct ggaccacggc gatgtcagcg gaagcctgta cggcgccagc
      901 ctcggtggcg gccggaacgg ccaggccacc atcgacgtca acggcgacgg cgtcatcagc
      961 ctgccggaaa ccaaggtggc cgtcctgccg ctggctcagc caggcatcgt cgactacagc
     1021 tatgactacg tctcctactc ggtcggcgtg aactatcgca tcgccgagcc ggtctcggtc
     1081 ttcggccgct acagccgcgg cggtcgcgct gcaggcgagc gtcagttgtt ccagccgcag
     1141 ttcaactcga ccaccggcca accggttgac gccgacacag cgtatggcga ggtcgaacag
     1201 gcggaagcgg gcgtcaagtt ccgccggccc ggtctggtcg tctacgccac cgggttctgg
     1261 gcttcgacgg gtgaaaacaa cacccagatc ggtgcggacg cctcgggtgc gcccaaggtc
     1321 atcagcttcg accggaccta cagtgccaaa ggcatcgagc tggagagcga gtacagctac
     1381 ggaccgttca gcgtcgctct gggcgccacc tatgccaagg cgagcatcga cggcgactcg
     1441 gcgaacgctg ccctggtcgg gaaccgcccc cgtcaccagc cggagctgtt ctataacgtc
     1501 cgtccgcagt tcgaacgcgg catgttcacg atcggcgcca ccatcaacgg cacgacgagc
     1561 agctatgcgc aggacgataa cgtgctggtt caacccggct acgtcatcgt taatccgtac
     1621 ttcttcgtgc ggcccctgcc gcgcattgaa ctgggactga ccgcgtacaa cgtgttcgac
     1681 gagatcgcga tcgtcagcat caactccagc gccatccccg cgtccggggt ggtctctgcg
     1741 cagactctga acggtcgcac catctccgcc tccctgcggt ataccttctg atcaaaaccg
     1801 gcggcagcca catctgcccg cggcagattt ctgccgcggg caggtgctgt tcggtggtgc
     1861 tctcatccga aggggatttc tcgggtcgcg actgaggtcg ggcccgtagc gacatatcct
     1921 gaccgttcca agcctgcgga ccgccacatg ctgatcgacc cgatgttcta cgcagtcgcc
     1981 ataccagccg tcatcctgct ggggctgacc aagggcggat tcgcgggtat cggggttgtc
     2041 gccgtgccga tgatggccct ggtcatctcg ccggtccagg ccgccgcgat catcctgccg
     2101 atcctgttgg ctcaggacgt ggtcggcgtc tggagctttc gcaagacctg ggacaagggg
     2161 ctggtgatcc tgatgctgcc gtcggcggcg gtgggaatcc ttgtgggatg gctgttggct
     2221 gcacgggtgg aggtggcggc ggtcgaactg gcgatcggcc ttatcgcggt gacgttctcg
     2281 ctgcagcagc tctgggccgg gcgggccgtc agggcggccg aagtgatcga ggccggcccg
     2341 gcgcgtcgct ggctgggcgt cctgtgtggc ctggtctccg gtttcaccag ccaggtggct
     2401 cacgccggcg ccccgccctt ccagatctac gtcctgcccc gccgcctgcc gcgtgacatg
     2461 ttcatcggca ccagcgccgt gtttttcgcc gcggtcaact ggatgaagct gccggcctac
     2521 atcgcgctcg gacagttcac cccccaggtg ctcaccacgg cggctgtgct gctgccgttg
     2581 gcgctggtct ctacggtggc cggcgtgtgg ctggtgcgac gggtgccggc caagggcttc
     2641 taccaggtga tatacgtcgt gctggctgcc gttggggtca agctggcctg gggcggggcg
     2701 tccggcctgc ttggctgacg gagcattggc cgctgagagc ctgctgcgtg aaacgaatag
     2761 cggcgccagg cttcagccca gcgtttcctc gccacccacg cggcgatctg ccggcggccc
     2821 ttctcgcgat caaatccgag gcccgtccgc gcagagggcg acagagcggg cctctgcacc
     2881 ttgtcagccc aaaccttcga gagcctggat cgccgcatcc ggcaggatta gcctggagga
     2941 cgcaaggttc tcggcgaggt ggagggttga tgaggtcccg gggatcagca gaatattcgg
     3001 cgagcgctgc agcagccagg cgagcgcaac ttgcatggcc gccacgccaa gatccgcagc
     3061 gactttggtc aggccctccg actgcagtgg cgagaagccg cccaagggga agaagggcac
     3121 ataggcgatt ccctcggcgg ctagctggtc aatgaaggcg tcgtcatccc tgcgagccag
     3181 attgtactgg ttctgcaccc cgacgatcgg gacgatgtga gaggcctgcg catactgggc
     3241 aggcgtaacg ttgctgaggc cgatgtgtct gatcagcccc tggttctgca gttcggcaag
     3301 ggccgaaagg ggttcctcga tcgaaccttc tgcggggccg ttcacgtcga acatagcgcg
     3361 gaaattgacc acgtcgagga catccaggcc gaggtttctc aggttatcgt gcaccgctga
     3421 agtgaggtca tcggccgaac aggcccggtg ccactcggcg ttcggaccgc gcacggcgcc
     3481 gaccttggtg acgatgagaa ggtcgtcagg atagggatgc agcgccttgt ggatcagctg
     3541 gttggtaacg tgcggcccgt agaaatcgct tgtgtcgatg tgattgacgc cagcgtctct
     3601 cgcggcgcgc agcaccgcaa gggctgcgtc aggatccctg ggtggtccga acacccccgg
     3661 gccggcgagt tgcatggcgc cgtagcccag acgattcacg gtccgtccgg cgaaagaata
     3721 ggtcgagttc gtcatgttcg ttctccttgg gaacaccaga caattagacc ttgcagcact
     3781 gcgcgataat cactcacaat ctgcacgccc tgtacgagtt tccgaacaat gcctcatgac
     3841 ctggctgatc tgaacgcatt cgtggcagtg gcgcgagcga gaggcttccg caatgcggcg
     3901 cgtatcgagg gagtcagcgc gtcagccttg agcgatgccg tgcgaaggct cgaaaccgga
     3961 ctaggtgttc gtcttctgca ccggacaacc cgcagtatca gcctgactga ggccgggagc
     4021 cgattgatcg atcgcctgga gcccgccctc agcgaggtcg acgccgccct ggccggagtc
     4081 ggaacactcg gcgacgaacc cgccgggacc ttgcgcctga atgtgccggt cagcgctacg
     4141 cgtcttgtgc tgccgcacat cgtacccgcc tttctggcgg cttacccgca gatccgaatg
     4201 gaggtcacag cagaggacag tttcgtggac gtcctggctg caggggccga cgcgggcatc
     4261 cggtatgacg agaggctgga acaagatatg attgcggtcc ctatcggccc gcgggttcag
     4321 cggttcgcct gcgccgccgc gcccttctac ctcgacactc acgggcgacc aggccatccg
     4381 cgcgacttgc ttggccacgc ctgtttgcgc ggacgctttt ccagcggcgt cagccccgcc
     4441 tgggagtttg agcaaggagg cgagacggtc atgatcgagg gtgatggtcc gttgctcgtc
     4501 aacatggggc cggcgattga tcttgccgtc gaggcggccg tcgcgggcgc cggagtgatc
     4561 tacgtgttcg aagactggat agcgcaacag ctcgccagag gggatctgga gcctgtgcta
     4621 caggactggt ggccaagttt ttcaggaccc tttctctact attccggccg gcggctggtg
     4681 cctccgcctc tgcgcgcctt catcgatttt atcagagacc ctcggtcgtc tggataacgg
     4741 ccatcatacc tgtcttctag ctgcaggttt gccgtaccat atcgagagag cctgcaagtg
     4801 gagggcgctg cgaccctcgc tactcaggcc cggctctcgc caacgccgcc gtctgttgcc
     4861 tcaggtcctg acggatgagc tttatgctgc tcatcctgtt cagcatttaa ggcggcggcg
     4921 gtgctgtcag tcgtacgtct cccgacagag cctcgaccag cgttgccaag cggtcgaggc
     4981 tgagcgctgg gccactcacc gtgctggtgc aaggccctcg cgtgacgggg gatgacggtc
     5041 gcgttattgc ctgccgggtg aacttgaaaa gactatggcg ttgggacgtc tcgtgcaccg
     5101 atcgctcgcg cgacgaccat cgccccgaac gccacgcttc cgtgagtgtc atctgcggga
     5161 tcggggtgcg cgaactcgga gcgcagcgtt ccctcacggt cgagcgtttc cgagcggagg
     5221 tctatgatgc cgacgcccag ggcggcgaac atggggggga ctgcgcgctc aatctccaga
     5281 aaggcccaac gcgaggcggt taccgggacg cggcgcggcg gaagggcgaa gctaacggtc
     5341 aatcccaggc ccaccaggtc ggcctgaaag gccagagcgc cggaaatcat cgcgcgcacc
     5401 gcggcttcga acgcggcctg gctcagatag cgcccttcga gtggtgagtt ggggatacga
     5461 aacccatccc agacctcttg ccgactgaga tagtgaaaat tacctccgaa ggtacacagg
     5521 acggggcagg gaatgcccgc cgcggccgta actcccaagc tatcgagaaa gctgcgataa
     5581 cgccggtcgg cttctgggtc gtgaaagcga aagtcgcggc cgtcgaaggc atagaagtcg
     5641 ccgttcaggt tgcgtccacc ctcgaccatg cctccaacaa catgccgagc catcgcagcc
     5701 gcggcgtccg ttgcgcaaac gccgtgcgaa tcgctgagga tcagaagttc agggggcggc
     5761 atagcgatcc agcaccattt cgtcgcaaac ggggtcgacc gaggcaggcg gaaggatcgc
     5821 cgcgaccggc acgcgcaaaa ggtgagggag gaagtgagtc atgacgaaat caacgcctct
     5881 gggactcact tcgcgcatgt tcggcgcgaa aaaatagccc cggaaggctg gagcagtgat
     5941 gatctcgtag gagggaaagt aatcatacag catctcgcgt gccgtcagtt cgcctgctac
     6001 tgcccgcagc accgacttgg aatgagttga cgccaccagc acatgatcgc cggttttggt
     6061 cgccgtaaga gggacaggtg acacggtcag caggatgcgc agccccggac gaaggctttg
     6121 gagcagatgg aaacactcgt ccatcgtctc cattacttcg gccatcgtct gattgtgaaa
     6181 gacataacga tcttgcgaat attcgcctgc aataatgcct gggcatgagg gatactccaa
     6241 acccg
//
LOCUS       DASVTM010000131         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_916595,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000131 DASVTM010000000
VERSION     DASVTM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916595"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VF633_03710"
     CDS             complement(<1..830)
                     /locus_tag="VF633_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEX8470196.1"
                     /translation="MRRLNASESVAPEPVGARKPRRAGRIVLIVAGVLLVLIAGLYIN
                     RRAAAQQILTSWLAQQGVHARVEVQRIELNGFTGRIEIGDPAHPDFVVDRAEVDYAIG
                     TPWSRAGLGLTPGRIRLVRPVLSARWKDGRISLGSLDPLIKKFTGRPPKPGSRSPLII
                     VEGGRLLLASEYGPVEVLADATVNDGKLMRLVARMPAASLKSGPMSITGLQGHIDLTT
                     TGDRVALTMQAASEAIQGPAGDGRDLDVAVTGDLPYPDLKTRRGDGRARLQIIANTGS
                     A"
     gene            complement(915..>2923)
                     /locus_tag="VF633_03715"
     CDS             complement(915..>2923)
                     /locus_tag="VF633_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470197.1"
                     /translation="TAQQAPTALTAIAGNRIGLADVTDLTEVGSLVAGMTVTNLGSGR
                     NKILLRGMSDGAFTGLTQSTVAIYLDLAPITYSAPDPDLKLVDIDRVEVLRGPQGTLY
                     GTGPIGGVVRIVTNRPNPYHLSLDLSATQSSTHSGGQNSDYSATANVPLLAGRLALRG
                     SIYKETFGGYINDVNLNLHRVNDGSRHGGRIAIGAQITPNWTVTGGYVQQLIDTEDTH
                     YVYRVLGGLTRANLVREPHRNHFDETYVTLNGEGNWGRFDASVADIRHSFTSRYDASS
                     ALINFGSFGRIGALDEDKDIHLVVGEMTLHSPETGPLRWLAGAFFSTSTTRAETTLSA
                     LLPLPVRTVYTENRDDNLDELAVYGEVSLDLTPSLTATAGARIYGFDYSAVSDVAQRT
                     LHRRFSGEGEVAGLSPKFGLNYRAGSDLNLYAQISQGHRTGGFNTAGPIGQMFIGRVG
                     MPAREYRSDTLWNYEVGAKARLWQGRVQARVAAFVSHWDDIQSDQFLPSGLAYAVNVG
                     DGANKGLEIEANWRATQNFEIRFNTLLADPEITRPSAVFNSRGDAGLPGVPAASANLN
                     ASYRRPIWRNLVFTTDGSVVYVGASRLTFDASRRYRMGDYVTGRLSTGIQGDRWSATA
                     FVDNPFDTEANTFSFGDPFRLPEALATTPLRPRTLGLTLRWSPW"
BASE COUNT          537 a          898 c          984 g          504 t
ORIGIN      
        1 gctgaacccg tgttggcgat gatctgaagc cgggcccggc cgtcaccgcg ccgggtcttg
       61 aggtcggggt agggcaggtc cccggtgacg gcgacatcga gatcgcgccc atctccagcg
      121 gggccctgaa tggcttcaga agccgcctgc atcgtcagcg ccacgcggtc gccggtggtg
      181 gtcaggtcga tgtgcccctg caagccggtg atcgacatgg ggccgctttt gagtgacgca
      241 gccggcatgc gcgcgaccag ccgcatcagc ttgccgtcgt tgaccgtcgc atcggccaga
      301 acttcgaccg ggccgtattc gctcgcgagc aacagccgcc cgccctcgac aatgatgagg
      361 ggagaacgcg agcccggctt tggtggtcgc ccggtgaatt ttttgatcaa cgggtccagc
      421 gagcccagcg agatccggcc gtccttccac cgggctgaca gaaccggccg aaccagcctg
      481 atccggccgg gcgtcaagcc aagtccggcc cgcgaccatg gcgtcccgat ggcatagtcg
      541 acctcggcgc gatcgacgac aaaatcaggg tgggcgggat cgccgatctc gatgcgaccg
      601 gtaaagccgt tcagctcgat acgctgaacc tcgacccggg catggacgcc ttgctgggcc
      661 aaccaactgg tcaggatctg ctgcgcggcg gcgcggcgat tgatgtagag gcccgcgatc
      721 aggaccagca gaacgccggc cacgatcagc acgatacgcc cggcacgtcg cggcttgcgg
      781 gccccgactg gctcgggggc gacggactcg gaagcgttca ggcggcgcat cgaggcgact
      841 taaagcaaat cggggtctgt gccgccatgc cgaatccggt cggggaggtg atcactgtca
      901 gccgacgccc aaactcacca aggagaccat ctcagcgtca gaccaagcgt gcgcgggcgc
      961 aaaggtgtcg tggcgagggc ctccggcaga cggaacggat cgccgaacga gaaggtattg
     1021 gcctcggtgt cgaacgggtt atcgacgaag gcggtcgccg accagcgatc accctggatc
     1081 cctgtcgaca gccgaccggt gacgtagtcg cccatcctgt agcggcgcga ggcgtcgaag
     1141 gtcagccgcg aggctccgac atagacgacc gatccatcag tcgtgaagac cagattgcgc
     1201 cagatcgggc ggcgataact ggcgttgaga ttggcagatg cggctggaac ccccggtaaa
     1261 cccgcatcgc cccgtgagtt gaagaccgcg ctcggacgcg tgatttcggg atcggccaac
     1321 agggtgttga agcgtatttc gaagttctgg gtggcgcgcc agtttgcctc gatctcaaga
     1381 cccttgttgg cgccgtcgcc aacattgact gcataggcca gtccgctggg caggaactga
     1441 tcgctctgga tgtcgtccca gtgggaaacg aatgcggcga cgcgagcctg tacccgaccc
     1501 tgccagagcc tggccttggc cccgacctcg tagttccaaa gggtatcgga ccggtactcc
     1561 cgggcgggca tgccgacgcg cccgataaac atctgcccga tcggtccagc ggtgttgaag
     1621 ccgccggtcc ggtgaccttg gctgatctgg gcgtagaggt tcaggtccga accggcccgg
     1681 tagttcaggc cgaatttggg ggacaggccg gcgacctctc cctcgcccga aaaacgacga
     1741 tgaagggtgc gttgggcgac atcggatacc gcactgtaat cgaagccata aatgcgggcg
     1801 ccggcagtgg cggtcaggct cggcgtgagg tccagcgaga cctctccata gacggccaac
     1861 tcgtccagat tgtcatcgcg gttctcggtg tagactgtcc gcacgggcag gggcaggagc
     1921 gctgacagtg tcgtttccgc ccgggtggtg ctggtcgaga agaaggcgcc ggccaaccag
     1981 cgcagcggcc cggtctccgg cgaatgcagt gtcatttcac cgacgacgag gtgaatgtcc
     2041 ttgtcctcgt ccagggcgcc gatccggccg aacgagccga aattgatcag ggcgctggag
     2101 gcgtcgtagc ggctggtgaa gctgtggcgg atgtcggcca ccgacgcatc gaaccggccc
     2161 cagtttccct cgccgttcaa ggtgacatag gtctcgtcga agtggttgcg gtggggctcg
     2221 cgaacgaggt tggcgcgggt tagtccgccg agcacgcgat agacgtagtg ggtgtcttca
     2281 gtgtcgatca actgctgaac atagccgcct gtgacggtcc agttcggagt gatctgcgcg
     2341 ccgatggcga tacgtccgcc gtgccgtgac ccgtcgttca ccctgtgcag gttcaggttc
     2401 acatcgttga tgtagccgcc gaacgtttcc ttgtagatcg atcccctgag cgccagacgt
     2461 cccgcgagca ggggcacatt ggccgtcgcg gaatagtcgc tgttctggcc accggagtgg
     2521 gttgacgact gcgtcgccga caggtcgagc gacagatgat acgggttcgg acggttcgtg
     2581 acgatacgaa cgacgcctcc gatggggccg gtgccgtaga gggtgccctg aggccccctc
     2641 aagacctcca cccgatcgat atcgaccagc ttgaggtcgg gatcaggagc actgtaggta
     2701 atgggcgcca ggtcgagata aatcgccacg gtggactggg tcaggccagt aaaggcgccg
     2761 tccgacatac cgcgcagcag aatcttgttc cgcccggaac cgagattggt cacggtcatc
     2821 cccgccacca gcgaacccac ttcggtcaga tcagtgacgt cggcgagccc gatccggttc
     2881 cccgcaattg cggtcaacgc ggtcggagcc tgttgcgccg tgt
//
LOCUS       DASVTM010000132         8154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_759238,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000132 DASVTM010000000
VERSION     DASVTM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759238"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF633_03720"
     CDS             complement(<1..357)
                     /locus_tag="VF633_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8470198.1"
                     /translation="MPRKFLVVVDDSPEFDAALRYAARRARSTGGRVALLAVIPDTSD
                     EHWAGVRGEIQRQQRAEAEALLTRLGAEAAERSGAPPIFLIEEGDAQAAIRKAVGEDP
                     EIKILVLASAGGGKSPG"
     gene            complement(467..943)
                     /locus_tag="VF633_03725"
     CDS             complement(467..943)
                     /locus_tag="VF633_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470199.1"
                     /translation="MSCGIALSLALMMTDPNVAVAAAQPPAVAGAPVSVASEPLFVDI
                     VSQSSALKALVDSWSAAKAADAPGFLTGADYTGFKTRAEHLSATDMQAHLILKERGTD
                     GDLKCILRGISEDIPKKVAAVEAATTPELRAVALSELSYLLNDNVEVITSPPQPEV"
     gene            1043..2653
                     /gene="pgi"
                     /locus_tag="VF633_03730"
     CDS             1043..2653
                     /gene="pgi"
                     /locus_tag="VF633_03730"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX8470200.1"
                     /translation="MFTMTADWTAFDAAAERDADARIVDQFTADPDRVSRLSVEAAGL
                     YLDLSKQSWTHEGFAACLDLARSAGVEAARTRLFGGEAINSTEGRAVLHPALRAARGS
                     NFIALGEAVSAEVDAVRADMAAYATAVRSGAETGATGKPFTAIVHIGIGGSDLGPRVV
                     WDALRPLDPQIDLRFVANIDPRDMAEALTGLDPETTLVVVVSKTFTTQETLANADAAK
                     AWLAAGLPEAGRDKHFIGVTAAPDKAAAFGCARTFAFRDWVGGRYSLWSAVSLSCVIA
                     LGPDAFEAMLAGAAAMDDHFVAAPLDKNAPVLLALAQIWNVDGLNRPARTVAPYAHAL
                     RRLPAFLQQLEMESNGKRVHRDGSPVTRQTCPVVFGEPGTNGQHAFFQQIHQGPQVVP
                     AEFVIVAKTHADAPESPLWSNALAQGQALMLGKTTAQAHAELLASGADAAEADRLAPH
                     KTFPGNRPSTAIVMDRLTPETLGALLALYEHKTFVEGVINDINSFDQWGVELGKVLAR
                     AILKDVDAGGPSAEMDPSTAGLLTRLLG"
     gene            complement(2709..3197)
                     /locus_tag="VF633_03735"
     CDS             complement(2709..3197)
                     /locus_tag="VF633_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470201.1"
                     /translation="MMKFSKIAAGAAAPVLALSLLAAGTADAQSYRNHDRDRGNDNTA
                     QNAIVGAAIGGIAGALLGNGDGRYVAGGALAGAAVGAASSSRNSRGSNNYYGGGYGYD
                     RGPDCGYYRNGQCYRNQGHWERDHGINSRDYNRNDRYERNDRYERNDRYDRRDYRYDR
                     RW"
     gene            3471..3755
                     /gene="groES"
                     /locus_tag="VF633_03740"
     CDS             3471..3755
                     /gene="groES"
                     /locus_tag="VF633_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX8470202.1"
                     /translation="MAFRPLGDRVLVKRVEEESKTKGGIIIPDTAKEKPQEGEVVSVG
                     PGVRDESGKVNALELKAGDRILFGKWSGTEVKLEGEDLIIMKESDVLGVL"
     gene            3812..5458
                     /gene="groL"
                     /locus_tag="VF633_03745"
     CDS             3812..5458
                     /gene="groL"
                     /locus_tag="VF633_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516094.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX8470203.1"
                     /translation="MAAKQVQFSTDAREKMLRGVNVLANAVKVTLGPKGRNVVIQKSF
                     GAPRSTKDGVSVAKEIELEDAFENMGAQMIREVASKTNDKAGDGTTTATVLAQSIVQE
                     GLKAVAAGMNPMDLKRGIDKAVTAVLAEIKASAKKVSNNSEIAQVGTISANGDVEVGE
                     MIARAMEKVGNEGVITVEEAKTAETELDVVEGMQFDRGYLSPYFITNADKMEVQLEEP
                     LILLFEKKLSSLQAMLPILEAVVQSGRPLLIIAEDIEGEALATLVVNKLRGGLRVAAV
                     KAPGFGDRRKAMLEDISVLTGGQVISEDLGIKLENVTIDMLGKAKKVTITKDDTTIVD
                     GVGDKEAIEARISQIKKQIEDTTSDYDKEKLQERLAKLAGGVAVIRVGGSTEVEVKEK
                     KDRVDDALNATRAAVEEGIVPGGGIALLKATKALEGLKGDNADQTAGIAIIRRAIQAP
                     IRQIVENAGVEGSIVVGKVLENPSLTFGFNAQTEEYGDLVAMGVIDPAKVVRTALQDA
                     ASVAGILITTEAAVADAPKKGGGGAPDMGGMGGGMGGMDF"
     gene            5989..6714
                     /locus_tag="VF633_03750"
     CDS             5989..6714
                     /locus_tag="VF633_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470204.1"
                     /translation="MDDAGITSAFARIADEWDKAEKLIKTAEVTRNEVVIASINELRY
                     AGRRLVDALRAADACRADPTCVKSKEDLNRYVSETLSFCQRALHDAVDAIILHVRTQI
                     GEYESEFGHAILADKFPAILEIKKTLRGAEQLVILSREDRAKRASDYDKLATELIPAL
                     LEHCDSLEANRSLLQELVAEKRKGEIRDRKVDRRFWITLIGGAILGVIGAVFLMVIKT
                     EYERSRALPAHTAQGQIDTRGKF"
     gene            7001..7180
                     /locus_tag="VF633_03755"
     CDS             7001..7180
                     /locus_tag="VF633_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470205.1"
                     /translation="MATYRTFIATYIMASERNGTLYTGVSSKLKTRVWKASPRDVRGV
                     QQGSWLHPAGVVRAA"
     gene            7185..7340
                     /locus_tag="VF633_03760"
     CDS             7185..7340
                     /locus_tag="VF633_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470206.1"
                     /translation="MTTAISHEKRIKGWKRDWKLALIETANPDWLDLAANWYAETDPN
                     WVPPPED"
BASE COUNT         1609 a         2596 c         2537 g         1412 t
ORIGIN      
        1 ccccggcgac ttgccgcctc cagccgaggc cagcaccagg atcttgattt cgggatcctc
       61 gcccacggcc ttgcggatcg ccgcctgcgc gtcgccctcc tcgatcagga agatcggcgg
      121 tgcgcctgag cgctcggcgg cctccgcccc tagccgggtc aacagcgcct cggcctcggc
      181 gcgttgctgg cgctggatct cgccgcgcac gccggcccag tgctcgtccg aggtgtcagg
      241 gatcacggcc agcagcgcca cccgcccgcc ggtcgagcgc gcacggcggg cggcgtagcg
      301 cagggccgca tcgaactcgg gactgtcgtc gacgacgacc agaaacttcc tgggcatggg
      361 gctctcgttg ggcacgaccg ctctctacgc gatcgcgcac actgaagcgc gttttcagcc
      421 gcgcgccaaa tcctgcgcga ctggatcggt tttaaatttc aggccgtcag acttcaggct
      481 gaggcggcga ggtgatgacc tcgacgttgt cgttcagcag gtatgatagt tccgacaggg
      541 ccacggcgcg cagttccggc gtcgtagccg cctcgacggc ggcgaccttc ttggggatgt
      601 cctcggaaat gccgcgcagg atgcatttca ggtcaccgtc tgtgccgcgt tccttgagga
      661 tcagatgagc ctgcatgtcc gtggccgaca ggtgttcggc gcgggtcttg aagcccgtgt
      721 aatcggcgcc ggtcaggaag cccggcgcat cggcggcctt ggcggcggac cagctgtcga
      781 ccagcgcctt cagcgccgag gactggctga cgatgtccac gaacagcggc tcggaggcga
      841 ccgagacagg cgctccggcc acggcgggcg gctgggctgc agccaccgcg acgttgggat
      901 cggtcatcat cagggcgagg ctgagggcga taccgcagga catgggaaac ctttccgtcg
      961 atcacttcag gagacaggcc ccgaataacc gttgaaccgg tttacgcccg acccgtaaac
     1021 gagaccttga tatcgacagg ggatgtttac catgaccgcc gactggaccg catttgacgc
     1081 cgccgctgaa cgcgacgccg acgcccgtat cgtcgaccag ttcaccgccg atcccgaccg
     1141 ggtgtcgcgc ctgagcgtcg aggccgccgg tctctatctc gacctgtcga aacaaagctg
     1201 gacccacgag ggcttcgcgg cctgtctgga tctggcccgt tcagccgggg tcgaggcggc
     1261 ccgcacgcgg ctgttcggcg gcgaggcgat caactccacc gaaggccgcg cggtgctgca
     1321 ccctgctctg cgtgccgcca ggggttcgaa cttcatcgca ctcggtgaag cggtctcggc
     1381 cgaggtcgac gccgtgcgcg ccgacatggc cgcatacgcc accgccgtcc gttcgggcgc
     1441 ggagacgggc gcgaccggca agccgttcac cgctatcgtc cacatcggca tcggcggttc
     1501 ggacctcggc ccgcgcgtgg tctgggatgc gctgcgtccg ctcgatccgc agatcgacct
     1561 gcgcttcgtc gccaatatcg atccgcgcga catggccgag gccctgaccg ggctcgatcc
     1621 cgagaccacg ctggtggtcg tcgtctcaaa gaccttcacg acgcaggaaa ccctcgccaa
     1681 cgccgacgcc gccaaggcgt ggttggcggc gggcctgccg gaagccggtc gcgacaagca
     1741 tttcatcggc gtcaccgctg cgccggacaa ggctgcggcg ttcggctgcg cgcggacgtt
     1801 cgctttccgc gactgggtcg gtgggcggta ttcgctgtgg tctgcagtca gcctgagctg
     1861 cgtcatcgcg ctcggccccg atgcgttcga agccatgctg gccggcgccg ccgcgatgga
     1921 cgaccacttc gtcgccgcgc ctctggacaa gaacgcgccg gttctgctgg cgctggccca
     1981 gatctggaac gtcgacggcc tcaaccgtcc ggcccggacc gtcgcgccct atgcccacgc
     2041 cctgcgtcgc ctgcccgcct tcctgcagca gctggagatg gagtcgaacg gcaagcgcgt
     2101 gcatcgcgac ggctcacccg taacgcgcca gacctgcccc gtcgtcttcg gcgagcccgg
     2161 caccaatggc caacacgcct tcttccagca gatccatcag ggcccgcagg tcgtccctgc
     2221 cgagttcgtt atcgtggcga aaactcatgc ggacgcgccg gagtccccgc tgtggtccaa
     2281 cgccctcgcc cagggtcagg ccctgatgct gggcaagacc acggctcagg cccacgccga
     2341 actgctggcg tccggcgccg atgcggccga ggccgaccgt ctggctccgc acaagacctt
     2401 ccccggcaac cgcccctcga cggccatcgt catggatcgc ctgacgccag agaccctagg
     2461 cgcgctgctg gccctctatg agcacaagac cttcgtcgag ggcgtcatca acgacatcaa
     2521 ctccttcgac cagtggggcg tcgagctcgg caaggtgctg gccagggcga tcctgaagga
     2581 cgtcgacgcg ggcggcccct cggctgagat ggacccctcg accgccgggc tgctcacccg
     2641 gctgctgggc tagctccaga cgcagaaacg ggcgccgaag cgcccgtttc ccgcttagct
     2701 acgtccgcct accagcggcg gtcgtagcgg taatcgcgcc ggtcgtaccg gtcgttccgc
     2761 tcatagcgat cgttgcgctc gtaccggtcg ttgcgattgt agtcgcggct gttgatgccg
     2821 tgatcgcgtt cccagtggcc ctgattccgg tagcactggc cgttgcgata gtagccgcag
     2881 tccgggccac ggtcgtagcc atagccaccg ccgtaatagt tgttcgaacc gcgtgagttg
     2941 cgcgaagacg aggccgcgcc gaccgcagcg cccgcgaggg ccccgccggc gacatagcgc
     3001 ccgtcgccgt tgccgagcag ggcgcccgcg ataccgccga tggccgcgcc gacgatggcg
     3061 ttctgggcgg tgttgtcatt gccgcgatca cggtcgtggt tgcgatagct ctgggcgtcg
     3121 gctgtaccgg cagccagcag gctgagagcc agaacgggag ccgcggcgcc ggcggcgatc
     3181 ttgctgaact tcatcatggt gcgtctcctc agaaacgatt gggccggcgg aatgccgact
     3241 gcatgatggg gttatggacc cccgcgcctg aacgacccgg gtttgcacca ttcattttca
     3301 gttcatcaca gcgtcacgtt ggaggcgctt tgtgacgttg ctgacccttg accgaacccc
     3361 tgcctcctcc tacttgcagc ttgcgttagc actcgtgggt cccggctgcc aaaacgccgg
     3421 ttcaggggcg ctttcgagaa cctttagaag tcataccgga gaatatgcag atggcgtttc
     3481 gtcctctcgg cgaccgcgtg ctggtcaagc gcgtggaaga agaatccaag accaagggcg
     3541 ggatcatcat ccccgacacc gccaaggaaa agcctcagga aggcgaagtc gtctccgtcg
     3601 gccccggcgt tcgtgacgaa tccggcaagg tcaacgctct cgagctgaag gccggcgacc
     3661 gtatcctgtt cggcaaatgg tcgggcaccg aggtcaagct tgaaggcgaa gacctgatca
     3721 tcatgaagga atccgacgtc ctgggcgtgc tgtaagcacc ctcgacctcg accttcttcc
     3781 cctgacaatc cagatttagg agccgcctca catggcagcc aaacaagttc agttctccac
     3841 cgacgcccgc gagaaaatgc tgcgcggcgt caacgttctc gccaacgccg tcaaagtgac
     3901 cctcggtccc aagggccgca acgtcgtgat ccagaagtcc ttcggcgctc cgcgctcgac
     3961 caaggacggc gtttcggtcg ctaaagaaat cgaactggaa gacgccttcg agaacatggg
     4021 cgcccagatg atccgcgaag tcgcttcgaa gacgaacgac aaggcgggtg acggcaccac
     4081 caccgcaacc gttctggctc aatcgatcgt gcaggaaggc ctcaaggccg tcgccgccgg
     4141 catgaacccg atggatctga agcgcggcat cgacaaggcc gtcaccgccg tcctcgccga
     4201 aatcaaggct tcggccaaga aggtctcgaa caactccgaa atcgcgcaag tcggcaccat
     4261 ctcggccaac ggcgacgtcg aagtcggcga aatgatcgcc cgtgcgatgg aaaaagtcgg
     4321 caacgaaggc gtcatcacgg ttgaagaagc caagaccgcc gagaccgagc tggacgtcgt
     4381 cgaaggcatg cagttcgacc gcggctacct gtcgccctac ttcatcacca acgccgacaa
     4441 gatggaggtt caactcgaag agcctctgat cctgctgttc gaaaagaaac tctccagcct
     4501 gcaagccatg ctgccgatcc tggaagccgt ggttcagtcg ggtcgtcccc tgctgatcat
     4561 cgctgaagac atcgaaggcg aggccctggc cacgctggtg gttaacaagc tgcgtggcgg
     4621 cctgcgcgtc gccgccgtca aggctcccgg cttcggcgat cgccgcaagg ccatgctgga
     4681 ggacatctcc gtcctgaccg gcggccaggt gatctcggaa gacctgggca tcaagctcga
     4741 gaacgtcacc atcgacatgc tcggcaaggc caagaaggtc acgatcacca aggacgacac
     4801 caccatcgtc gacggcgtgg gtgacaagga agccatcgaa gcgcgtatct cgcagatcaa
     4861 gaagcagatc gaagacacga cctccgacta cgacaaggaa aagctgcagg aacgtctggc
     4921 caaactggcc ggcggcgttg ccgtcatccg cgtcggcggt tcgaccgaag tcgaagtgaa
     4981 agaaaagaaa gaccgcgtcg acgatgcgct gaacgccacg cgtgcagccg ttgaagaagg
     5041 catcgtcccc ggcggcggca tcgcgctgct caaggcgacc aaggctctgg aaggcctgaa
     5101 aggcgacaac gccgatcaga ccgccggtat cgcgatcatc cgtcgcgcca tccaggcccc
     5161 gatccgtcag atcgtcgaga acgccggcgt cgaaggttcg atcgtggtcg gcaaggtgct
     5221 cgagaacccc tcgctcacct tcggcttcaa cgcccagacg gaagaatacg gcgacctggt
     5281 cgccatgggc gtcatcgacc ccgccaaggt cgtccgcacg gccctccagg acgccgcctc
     5341 cgtggccggc atcctgatca ccaccgaagc ggccgtggcc gatgctccta aaaagggtgg
     5401 cggcggcgcc cccgacatgg gcggcatggg cggcggcatg ggcggcatgg acttctagtc
     5461 cacgctactc acacgctgaa caaagagagg gcgggtccag caatgggccc gccctttggc
     5521 accatttccg ccataaagac ggtgtgttat ccgtgctcca aacgacacaa ttcaatagtt
     5581 atccacacgt tgttattcca acaccgacac aacatgcggt gttcagcacc ctctgaaacc
     5641 aacatgttga ttaacgtaac gttggtttgg aacaacctat tgtcgtggag gctttctgcg
     5701 actagtgacg cgcacctcca atcagtgcag cgtggctgat agggaacggg attgaaccat
     5761 gggccataat tttttcgcga gcagttcgcg cgcgaacgat gaacgcgttg ccaagttcga
     5821 aaagcgcctc caggacaacc gtatgttggt tgtacgccgc ttcgcacgtg gaaacgttgc
     5881 gcttcaacac ggtcgagtgc taactcaaga ccagctggaa aaagaacgtg agtcgctgcg
     5941 cgcgaatctg tcttcggcta gctgctgatc aactaactag gctcgagcat ggacgacgcc
     6001 ggcattacct cggcgttcgc gcgcattgcg gatgagtggg acaaagctga aaaactcata
     6061 aagactgcgg aagtgacccg aaatgaggtc gtaatcgcgt cgatcaatga gttacgttac
     6121 gccggacgcc gattagtcga tgctttgcgt gccgcagatg catgccgagc agatccaact
     6181 tgcgtgaaaa gcaaggaaga cttgaatcgc tacgtcagcg agaccttatc attttgtcaa
     6241 cgagcgctac acgacgcagt tgatgcgata atcctgcacg tcagaactca gatcggagaa
     6301 tatgaatcag agttcggcca cgcaatactg gcagataaat ttcctgcaat tttagagata
     6361 aagaaaacgc tgcggggcgc agagcagctt gtcatactat caagagaaga cagagctaag
     6421 cgcgcaagcg attacgataa gctagctaca gaattgatcc ctgccctgct tgagcattgc
     6481 gatagcttag aggctaaccg gtcgttgctc caagaacttg tcgcggaaaa gcgaaagggt
     6541 gaaattcgcg atcgcaaagt tgaccgccga ttctggataa cgcttatcgg cggtgcgatc
     6601 ctcggagtta tcggtgcagt ctttctgatg gtaatcaaaa cggaatatga gcgctctaga
     6661 gctctaccgg cccatactgc tcaaggtcag atcgatacac gcggcaagtt ttaacaaagt
     6721 gaagatcaca ttgttcgagg tgacattggt ttttctgaga cattgcacag cgaattaagg
     6781 atatgcgtac taactaactc cactgcccca ttccgtgcac agcatagaga acaatctgct
     6841 tgggcccgaa cttatagcgg acgagatgtt tcaaccaccc acttgaacag gtcgctttcg
     6901 gcgccgctgg gttgccccca tcagtcgagc aacgacagtg gcgatgacgg ggggcggcgg
     6961 tgatcaacgg ggccgtggga agatcggcta gattgtgtgg atggcgacct accgaacgtt
     7021 tatcgccacc tacatcatgg ccagcgagcg gaacggcacg ctctacaccg gcgtgtcctc
     7081 gaagctgaag acgcgggtgt ggaaagcatc gccaagggac gttcgagggg ttcagcaagg
     7141 atcatggctg cacccggctg gtgtggttcg agccgcatga acgggtgacg accgccatct
     7201 cgcacgagaa gcggatcaag ggctggaaac gcgactggaa gctggcgctg atcgagacgg
     7261 ccaacccgga ctggctggat cttgcggcca actggtacgc ggagacagac cccaactggg
     7321 tcccgccacc tgaggactaa agccccgtca ttccgggcgc agcgcagcga agacccggaa
     7381 cccagcggcc tgcgccagca ggaccctgtc gcggactcgg cgtttcaaca gcgtgcatgg
     7441 aaaggtcgcc ttcggccgcc gctgggttgc ccgatcgagt cgggcaatga cggggtcagg
     7501 gggcgagcgc ccgcaattcc ccgcaccaat cccctacccc gcccgggttt gcgcctttcc
     7561 cacccctttc gactccgcac accccaaacc gcgcgtatgc ttctccctgt cccgtcgcga
     7621 ggggaaggcg tcctgggccc aggagccgga cgtgcggcgg ggtcgatcgc ggggttgccg
     7681 gggaatagag agccccgggt ccggcagtgc ttctgggcgc tgccgcctgt cgtgggctga
     7741 gccgagcgga gcagggacga accgggttca accagccggc aaggaaaatg ggccgagccg
     7801 gtcccggctg aagagattcc cacccccgcg gcgcttgcga gaaccgatcg aacggagcac
     7861 gggcgaccac gccgaaactc ccccttagag acggcgaacg gcgacgttct ccgtcaacgg
     7921 tatccgggcg agatcgcccg tgctccgtcg cttccctgcc gcccaccggg tttcccggcg
     7981 gggtacacaa acatgaactt cagcgctggt gcgcgtgagg acgagaatct gtgcgtgagt
     8041 gtcctcacca tcgctctcgc gatggggagg gggaccgcgt cttcggtgga ggggttcttg
     8101 aagcgaatgc ctacgacggg ggttcaagag cccctccgtc acgtcgctga agga
//
LOCUS       DASVTM010000133         5105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_172352,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000133 DASVTM010000000
VERSION     DASVTM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172352"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1144)
                     /locus_tag="VF633_03765"
     CDS             complement(53..1144)
                     /locus_tag="VF633_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX8470207.1"
                     /translation="MTDLKTPDPLKNVIHRGDCLEILRGLPSKSVDMVFADPPYNLQL
                     GGDLLRPDNSKVDAVDDDWDKFDSFAAYDSFCRDWLKECRRVLKDEGSLWVIGSYHNI
                     FRLGVAIQDMGYWVLNDIIWRKSNPMPNFKGTRFTNAHETLIWAAKSRDQKRYTFNYD
                     AMKAFNEDTQMRSDWTFPLCTGEERIKGEDGKKAHPTQKPEALLHRIILAATRPGDVI
                     LDPFFGTGTTGAAAKRLGRHFVGIERDETYAKVAETRIAGVIPAAPDDLKVMGSKRAE
                     VKVPFGALVEAGMLAPGDILYGPKESNQARVRADGSLVSGEFTGSIHKLGAILENAPA
                     CNGWTYWRFKTDQGFKSIDALRTEIRLTM"
     gene            1261..1527
                     /locus_tag="VF633_03770"
     CDS             1261..1527
                     /locus_tag="VF633_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX8470208.1"
                     /translation="MIEFDEAKAVANLAKHGLPLSLAEEMDMFDAVILADERKDYGEA
                     RFRAFGRIEGRWHCLVFTLRGDAIRAISLRHAHAKEIKRHAPKR"
     gene            1511..1795
                     /locus_tag="VF633_03775"
     CDS             1511..1795
                     /locus_tag="VF633_03775"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX8470209.1"
                     /translation="MPRNVDLSDPNNPEWIDADFARARPASEVLSPAQLVQFKRSPGR
                     PKLEKPKVAVSLRLDPDVLNFFKATGPGWQARLEDVLRGAISDNPSVRQG"
     gene            1898..3568
                     /locus_tag="VF633_03780"
     CDS             1898..3568
                     /locus_tag="VF633_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8470210.1"
                     /translation="MRLTLSLAALMTGVAMSTLAVAQTAAPAFDPAKLSEHIRILSDD
                     SYEGRGIATEAEPKVIKYLSEQYAAAGFEPGGDPQADGTRLWTQAVALNKFVVSDFAA
                     QLKVGDWTLPLEQGQQVVASTRLPTDAGHVMLMNAPLVFVGYGIRAPERNWDDFKGMD
                     LKGKILVVLINDADFEEPALDTFGGKAMTYYGRWTYKYEEAARQGAAGMLIVHEDAPA
                     SYGWNTVRNSWTGPQFDIVRADPSKDRVAVEGWIQRDVAVQLFQHSGLDFEALKAQAR
                     SRDFRPVELTGAGLDVMFNEAASRIESHNVVARLAGTTHPDETVLYTAHWDHIGVGTP
                     DANGDAIFNGAVDNASGTAGLLELARLFGAAPRTQRSIVAISFTGEESGLLGSTFYAA
                     NPLYPLAKTVGGINMDSANVYGRTSAMSIIGFGQSDFDERVTAKVESQGRAIEPDGNT
                     QAGYYFRSDHFPLAKEGVPMAYAESGGTFRDEPVGARIAARDAYTEKRYHQADDEWSA
                     DWDYRGQMEDLTVYYDIGRALADSRDWPAWKTGSEFGPARARTADQRE"
     gene            3649..>5105
                     /locus_tag="VF633_03785"
     CDS             3649..>5105
                     /locus_tag="VF633_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8470211.1"
                     /translation="MFARLAYSKLACGVAASALLFSTMPALAQDFSAERLSDHIKILS
                     DDSFEGRFPGKIGEQKTLEYLQAQYEAMGLEPGGPDGQWLQPVDLLRLTPTRPPVISW
                     TGADGVAHVVDAGITARAGAAESQVKLAGLPIVFAGYGVVAPERKWDDYGDMDLTGKV
                     VILLNGQPAAFGADPNFYGSSTHKTEEAMKRGAVGVLTLYEATGGRGMRGGGRPRMTI
                     TGAHETAFNGIMTLDAVTAMAGSMQGDVQSIIASAKLGGAFKAHDTGLKLSVDIDETG
                     EVIHSHNLLAKITGTTRPDEYLVYSAHWDHVGTAADPDATGDRIFNGAWDNASGTSGV
                     VEMARAFKAGPAPERTVVFLHVTAEEQGLLGSEWYATHPVYPLEKTAADINIDMLPFT
                     PATKTIAIFGIGKSTLEDDLSRLAQREGRNVVGDGEPEQGYYYRSDHFNFAAGGVPAL
                     MPWTGVDFVEGGEAVGRPYYQAQMAKYYHKLDDEWR"
BASE COUNT          920 a         1574 c         1681 g          930 t
ORIGIN      
        1 cgcccgggcg acaggtcaaa tgaagcccgc gtaatctacg cgtgtttgcg gactacatcg
       61 tcagccggat ttccgtgcgc agggcgtcga tggatttgaa gccctgatcg gtcttgaagc
      121 gccagtaggt ccagccgttg caggccgggg cgttctcgag gatggcgccg agtttgtgga
      181 tcgagccggt gaactcgccc gacaccagcg agccatcggc gcggacgcgg gcctggttgg
      241 attccttggg gccatagagg atgtcgccgg gggccagcat tccggcctcg accagcgcgc
      301 cgaacggcac cttcacctcg gccctcttcg agcccatgac cttgaggtcg tcgggcgcag
      361 cggggatgac gccggcgatg cgggtctcgg cgaccttggc gtaggtctcg tcgcgctcga
      421 tgccgacgaa gtggcggccc agacgcttgg cggcggcgcc agtggtgccg gtgccgaaga
      481 aggggtcgag gatcacgtcg ccgggacggg tcgccgccag gatgatccgg tgcagcaggg
      541 cctcgggctt ctgggtcgga tgggccttct tgccgtcctc gcccttgatg cgttcctcgc
      601 cggtgcacag cgggaaggtc cagtcggacc gcatctgggt gtcttcgttg aaggccttca
      661 tcgcatcgta gttgaaggtg tagcgcttct ggtcgcggct cttggcggcc cagatcagcg
      721 tctcatgagc gttggtgaac cgggtgccct tgaagttcgg catcgggttc gacttgcgcc
      781 agatgatgtc gttgaggacc cagtagccca tgtcctgaat ggcgacgcca aggcggaaga
      841 tgttgtgata gctgccgatg acccacagcg agccttcgtc cttgaggact cggcggcatt
      901 ctttcagcca gtcgcggcag aaactgtcat aggcggcgaa gctgtcgaac ttgtcccagt
      961 cgtcatcgac tgcatcgacc ttcgagttgt ccggacgcag caggtcgccg cccagctgga
     1021 gattataggg cggatctgcg aagaccatat ccaccgactt cgacggcagg ccgcgcagga
     1081 tctcgaggca gtcgccacga tgaatgacat tcttgagcgg gtctggggtc ttcagatcag
     1141 tcacgcgcgc agctccagga gagtagaacc gcgatggtga atccttgtgg ttaaggtcgg
     1201 gtaaccgccg cgccggattg atgtctggct tgccgatgat ttctgtagta cgaaaactct
     1261 atgattgagt tcgacgaggc caaggctgtc gcgaacctcg ccaaacacgg tctgcctcta
     1321 agcttggctg aagagatgga tatgttcgat gcagtcatct tggccgatga acgcaaggac
     1381 tacggcgagg ctcggtttcg agctttcggc aggatcgagg gcaggtggca ctgcctcgtc
     1441 ttcaccctgc gcggagatgc gatccgggcg atcagtctgc gccatgctca tgcaaaggag
     1501 atcaaacgcc atgccccgaa acgttgactt gtctgatccg aataatcccg aatggatcga
     1561 cgcggatttc gcgcgtgcgc gtcccgcgag cgaggtcctg agcccggctc aattggttca
     1621 attcaaaagg tcgcctgggc gtccgaagct ggagaagccc aaggttgcgg tgtcgttgcg
     1681 actggatcct gacgtgctga acttcttcaa ggcgacaggt ccggggtggc aggcccggct
     1741 ggaggacgtt ctacgcggcg ccatcagcga caacccttcg gtgagacagg gctgacaagc
     1801 tggttcgacg ctccgcaaaa acggaagtcc tcgcgacgca tcgacacctg cgttcggtct
     1861 gaatagggtc acagccaaag ccagccccgg agcccgcatg cgtctcaccc tttctctcgc
     1921 cgcgctgatg accggcgtcg ctatgtccac gcttgcggtg gcgcagacgg cggctcccgc
     1981 cttcgatcca gcgaaactgt ccgagcatat ccgcatcctg tctgacgaca gctacgaggg
     2041 ccggggcatc gccaccgagg ctgagcccaa ggtcatcaaa tatttgagcg agcagtacgc
     2101 cgccgctggc ttcgagcccg gtggtgatcc gcaggctgac ggcacgcggc tgtggacaca
     2161 ggctgtggcg ctcaacaagt tcgtggtcag cgactttgcg gcccagctca aggtcggcga
     2221 ctggaccctg cctctggagc agggccagca ggttgtggcc tcgacccggc tgccgaccga
     2281 cgcgggccac gtcatgctga tgaatgcgcc gctggtgttc gtgggctatg gcattcgcgc
     2341 gcccgagcgg aactgggacg acttcaaggg catggacctg aagggcaaga tcctggtcgt
     2401 gctgatcaac gacgccgatt tcgaggagcc tgctctcgat acgttcggcg gcaaggcgat
     2461 gacctattac ggtcgctgga cctacaaata cgaagaggcg gctcgtcagg gcgcggcggg
     2521 catgctgatc gtccatgagg acgcgcctgc atcctatggc tggaacacag tgcgcaactc
     2581 gtggaccggg ccacaattcg acatcgtccg cgctgatcct tcgaaggatc gcgtcgccgt
     2641 ggagggctgg atccaacgcg acgtcgccgt ccagttgttc cagcactcgg gcctcgattt
     2701 cgaagccttg aaggctcagg cgcggagccg agacttccgt ccggtcgaac tcaccggcgc
     2761 cgggctggac gtcatgttca acgaggctgc ctcgcgcatc gagagccaca acgtggtggc
     2821 tcgactggcg ggcacgacgc atccggacga gaccgtcctc tatacggcgc actgggatca
     2881 catcggggtc gggacgccgg acgcgaacgg cgacgccatt ttcaacggcg cggtcgacaa
     2941 cgcctcgggc acggcgggtc tgctggagct tgcgcgtctg ttcggcgcgg ccccgcgcac
     3001 ccagcgctcg atcgtggcca tcagcttcac cggcgaggaa agcggcctgc tgggctcgac
     3061 cttctatgcg gccaacccgc tatatccgtt ggccaagacc gtcggtggca tcaacatgga
     3121 ctcggccaac gtctacggcc ggacctcggc catgagcatc atcggctttg gccagtccga
     3181 tttcgacgag cgagtcaccg ccaaggtcga gtcgcagggc cgcgcgatcg aacccgacgg
     3241 caacacccag gccggctact atttccgctc cgaccacttc ccgctggcca aggaaggcgt
     3301 gccgatggcc tatgccgaaa gcggcgggac cttccgcgac gaacccgttg gcgcccggat
     3361 cgccgcccgc gatgcctata ccgagaaacg ctaccatcag gccgacgacg aatggtccgc
     3421 cgactgggac tatcgcggcc agatggagga cctgacggtc tactacgaca tcggccgcgc
     3481 tttggccgac agccgcgatt ggcccgcgtg gaagaccggc tcggagttcg gcccggcccg
     3541 cgcccgcacc gccgaccagc gcgagtaatc gtcctcatta cgaaaacgta acggacggtc
     3601 cggctgggcc gccctaaatg cttgccttag actgttctgg agttcctgat gtttgctcgc
     3661 ctggcctatt cgaagctcgc ctgcggtgtc gccgcttccg cgctgttgtt ttccaccatg
     3721 ccggccttgg cgcaggactt ctcggccgag cgcctgtcgg accacatcaa gattctgtcg
     3781 gacgacagtt tcgaaggccg tttccccggc aagatcggcg aacagaagac actcgaatat
     3841 ctgcaggccc agtatgaggc catgggtctg gaaccgggtg gtccggacgg ccaatggctg
     3901 caaccggttg atctgttgcg tctgacgccg acgcgcccgc cggtgatcag ctggaccgga
     3961 gccgatggcg tggctcatgt cgttgacgca ggcatcaccg cgcgcgccgg cgccgccgaa
     4021 agccaggtga agctggctgg cctgccgatc gttttcgccg gatacggtgt cgtcgcgccc
     4081 gagcggaagt gggacgacta cggcgacatg gatctgaccg gcaaggtcgt catcttgctc
     4141 aacggccagc ctgcagcgtt cggggctgac ccgaatttct acggctcttc gacccacaag
     4201 accgaagagg cgatgaagcg gggcgccgtg ggcgtgttga cgctttatga agccaccggt
     4261 gggcgcggga tgcggggcgg tgggcggccg cgcatgacga tcacaggcgc acacgagacg
     4321 gcctttaacg gcatcatgac gctcgacgct gttacggcga tggctggatc gatgcagggc
     4381 gacgtccagt cgatcatcgc cagcgccaaa ctgggcggag ccttcaaggc gcacgacacg
     4441 gggctgaagc tcagcgtcga catcgacgag accggcgagg tcatccactc gcacaacctg
     4501 ctggccaaga tcaccggcac gacgcggccg gacgaatatc tggtgtattc ggcccactgg
     4561 gatcatgtcg gcacagcggc tgatccggac gccacaggag accgcatctt caacggcgcg
     4621 tgggacaatg cctcggggac gtcaggcgtc gtcgagatgg cgcgcgcgtt caaggccggt
     4681 cctgcgcctg aacgcaccgt cgtcttcctg cacgtcactg ccgaggagca gggcctgctg
     4741 ggctcggagt ggtacgcgac ccatccggtt tatccgctgg agaagaccgc ggccgacatc
     4801 aacatcgaca tgctgccctt caccccggcg acgaagacca tcgccatctt cggcatcggc
     4861 aagtcgacac ttgaggatga cctttcgcgt ctggcgcagc gcgagggccg caacgtcgtg
     4921 ggcgatggcg agccggagca ggggtattac taccggtcgg accacttcaa ttttgcggct
     4981 ggcggggtgc ccgccctgat gccgtggacc ggggtcgatt tcgtcgaggg cggcgaggct
     5041 gtcgggcggc cctattacca agcccagatg gcgaaatatt accacaagct cgatgacgaa
     5101 tggcg
//
LOCUS       DASVTM010000134         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_67957, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000134 DASVTM010000000
VERSION     DASVTM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67957"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1102
                     /locus_tag="VF633_03790"
     CDS             308..1102
                     /locus_tag="VF633_03790"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX8470212.1"
                     /translation="MANVLKHPLFGPAFVIGVALSGFFDGILLHQVLQWHHLLSLVPG
                     ETIQRLTTQILADGLFHVLMYGIALAGGAWLWTRRHALAGDGASPQFWAGVIIGFGTW
                     NIVDVTLFHWLLEIHHIRLDVAQPLVWDACWLLALGVGPVVVGALVGRKTSGGSSGAA
                     SAVVVILTVLVCGGWALRPPAGSDETLAVFAPDADPARVMQAVAAVDGQLVRVVGEGS
                     IVVLRLTDKGQSWKLYRHGAWLVSATAPAGCLTWSTARSGSTAERA"
     gene            complement(1468..1776)
                     /locus_tag="VF633_03795"
     CDS             complement(1468..1776)
                     /locus_tag="VF633_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470213.1"
                     /translation="MLEHSTRQILIRNAGSSRAAEIATRYAGEGWIVSLEISEEDTPG
                     NELVVQVWDAGGHVHVLSKAVCPVRDGRRFGRGEKKFDLVVDAGATEAACGRRKPLAG
                     "
     gene            complement(1828..2460)
                     /locus_tag="VF633_03800"
     CDS             complement(1828..2460)
                     /locus_tag="VF633_03800"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8470214.1"
                     /translation="MSDRIHTLEELSAVQHRRRRSVAIGLILVMGIALCTRSRWQADD
                     FPRLLIEDLGLALIAVAVVGRAWCSLYIGGRKAAELITTGPYSLTRNPLYLFSFVGAF
                     GVGAQSGSLVLGVVFLLATMAIFVPLIVREESFLANAMPGLFHAYRRLTPRLWPRPAL
                     WRSPDEITVRPALFLRTLSDGLPFVLAWPLFNGIENLQQIGLLPVIARWP"
     gene            complement(2658..3515)
                     /locus_tag="VF633_03805"
     CDS             complement(2658..3515)
                     /locus_tag="VF633_03805"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HEX8470215.1"
                     /translation="MHSSQTHRHPGVLQTSSPGQLSSRLGAVAGVLCATTLAACHTTA
                     APSSGYLSAYDGMEKAGRTGVALTQRRDDVASDNVRMVFIEPAAFAPNVGAALSDNER
                     TMVLSEVDRQICFEVSERFTIASSPAPDTATIRTAVVRLQPTGRIGSAVSAAVGYFVP
                     VVSLRPPMTTGGLAVESELLEAGTGRQIAAIAWARNAQYVGRDSASLSRVGDALQMAE
                     PMGDAVGDAFSSKARKVNDIPAVDPCAAFGSRRDIGRTAAGIAFGAVTGLYSPEVAGD
                     GAKRSSSEP"
BASE COUNT          701 a         1220 c         1255 g          676 t
ORIGIN      
        1 gatcccgaca atctggagtg agacaggacc acgcagtcta ctgcagcggc gcccattcga
       61 tccgccccaa ggcggtcgac ggcacgaatg tcacgacctg cacgattgac gacgccgggc
      121 atggacaccc gagcagatcg agaccgtcgt cgcaccgttc atcggctcta ggcctcgcgc
      181 accacaacac ggtggggatt ggactgggac tggcagtcgc caagggcgtc aggtgaagcc
      241 cagcgtcgct tcttcgacat aggaacgacc gtgtgcgccg ggatttcacc cgaagggaga
      301 actggatatg gcaaacgtat tgaagcaccc gctgttcggg ccggcctttg tcatcggcgt
      361 ggcgctgagc ggcttttttg atggaatact gctccatcag gtcctgcagt ggcaccacct
      421 cctcagcctc gttcccggcg agaccatcca gcgtctgacc acgcagatcc tggccgacgg
      481 tttgtttcac gtcctgatgt acgggatcgc actggctggc ggcgcctggc tctggaccag
      541 gcgtcacgcc cttgctggtg atggggcgtc gccgcagttt tgggccgggg tcatcatcgg
      601 cttcgggaca tggaatatcg tcgatgtgac cctgttccat tggctcctgg aaatccacca
      661 catccgtctg gatgtcgccc aacctctcgt ctgggatgct tgctggctgc tcgcgctagg
      721 tgttgggccg gttgtcgtcg gcgccctggt tgggagaaag acgagcggcg ggtcgtcggg
      781 cgcggcctcg gcggtcgtgg tcatcctcac cgttctggtt tgtggtggtt gggcgcttcg
      841 cccgcctgcc ggatctgacg agaccttggc ggtcttcgcc ccggacgccg atccggcgcg
      901 ggtcatgcaa gccgtggccg cggtcgacgg ccagcttgtg cgtgtggtgg gagaaggctc
      961 aatcgtcgtg ctgcgcctga ccgataaggg tcagtcctgg aagctatatc gccacggcgc
     1021 ctggctggtc agcgccactg cgcccgccgg gtgtctcacc tggtcaacgg cacggtccgg
     1081 atccacggct gagcgagcct gacgtcacgc gcgacccagc atggcgtcag tcgacgcgtc
     1141 cctgccagga acctgccggt tgcgagcacg cgggcctgat ggatcccccg actggtaggc
     1201 ggtttccgcg cggggaaacg atcccggcct ttgatcgata tgacatccac gacacggacg
     1261 atccccgcgc cgttgatcga ggcgttaagc tgacaggcat tcggccgagc ttcatcctgc
     1321 cgatcatgtt cctcggcaga agcttggact gggaagcaac gagatcggca tcggaaccat
     1381 cggccgccca tccgatggat cactcacgca caagaacagc agatgttcga cggtatcgag
     1441 aggccgcgcg ccacctacgt cgagccgcta ccccgctaac ggttttcgtc gtccacaggc
     1501 agcctcagtc gctccggcgt ccacgacgag atcgaacttc ttttcgcctc ggccaaaccg
     1561 tcgcccatcc cgaaccgggc aaacggcctt actcagtacg tggacatgcc cgcctgcgtc
     1621 ccacacctgc acgacgagct cgttgccggg cgtgtcttct tccgaaatct ccaggctgac
     1681 gatccagccc tcgcctgcgt atcgggtggc gatttcagca gcacgggaag aacctgcgtt
     1741 gcggatcagg atctgtctcg tcgaatgctc gagcatgact tcactacctg attccccgga
     1801 cggttgagtt gatcgggtct gcatcgatca gggccagcgg gcgatgaccg gcagaaggcc
     1861 gatctgctgc aggttctcga ttccgttgaa caatggccag gccagaacga aaggcaggcc
     1921 gtcggaaagg gtcctcagga acagggcggg tctgacggtg atctcatccg gcgaccgcca
     1981 aagggccggc ctgggccaca gccggggcgt caggcggcga taggcgtgga acaggcccgg
     2041 catggcgttg gcgagaaagc tttcctcacg cacgatcaac ggcacgaaga tcgccatcgt
     2101 cgccaacagg aagaccactc caagaacaag gctacctgac tgggctccca ccccgaacgc
     2161 gccgacaaaa ctgaacaggt acaggggatt gcgggtaagc gaatacgggc ccgtggtgat
     2221 cagctctgca gcctttcgcc cgccaatgta aagcgaacac caggcccgcc ctacaaccgc
     2281 aacggcgatg agcgccagcc cgagatcttc gatgagcagg cgcggaaaat cgtccgcttg
     2341 ccaccgggag cgggtgcaca gcgcaattcc catgacgaga atcaatccta tggcgacgga
     2401 tcggcggcgt cggtgctgga cggcgctgag ttcctccaga gtgtggatgc gatcgctcat
     2461 cccggcgcac cagccaaggc ggatggtggc cgaagtgcgg gggccaggac gaccgtggca
     2521 aagacggcga atgattgtag cgaacctggg tccgccaagg gtggcttgaa atacatgaag
     2581 agtccgggga gtcggaggga caagggtctc ggcgccgggc gggtcagcgg aaacgagatc
     2641 gcccggcgcc ggtggcgtca gggctcggac gagctccgct tggcgccgtc gccggcaacc
     2701 tctggggaat agaggccggt gaccgcaccg aaggcgatgc cggcggccgt ccttccaata
     2761 tcgcgccgcg agccgaaggc ggcgcagggg tcgacggccg gaatgtcgtt gaccttgcgc
     2821 gccttgctgg aaaacgcgtc gcccacggca tcgcccatcg gttccgccat ctgcaaggcg
     2881 tcccccaccc gcgacagcga cgccgagtcc cgtcctacgt actgggcatt gcgagcccag
     2941 gcgatggcgg cgatctgacg tccggtcccg gcctccagca gttcggattc caccgcaagc
     3001 ccaccggttg tcatcggcgg gcgcaggctg acgacaggca cgaaatagcc caccgcagcg
     3061 ctgacggccg atccaatccg tccggtcggc tgaaggcgca ccacggcagt gcggattgtc
     3121 gccgtgtcgg gggcgggcga ggaggcgatg gtgaaccgct cgctgacctc gaagcagatc
     3181 tggcgatcga cctcgctaag gaccatcgtc cgctcattgt ccgacagggc ggcgccgacg
     3241 ttcggagcaa aggctgcagg ctcgatgaag accatacgaa cattgtcgga cgccacatcg
     3301 tcgcggcgct gggtcagggc gacaccggtt cggccggctt tctccatacc gtcataagcg
     3361 ctgagatagc ctgaactggg agccgccgtc gtatgacagg cggccagcgt cgtcgcgcag
     3421 aggacgccgg caacggcccc cagtctggag gacagttggc cagggctgct cgtctgcagg
     3481 acgccggggt ggcgatgggt ttggctgctg tgcattgctg aactctcgat ctgggggcgc
     3541 cgcgttcggc gctcgtcaac gacgtagtca cgcgtcgaac ctctcaatcg gaatcgaata
     3601 ttatttcatc gaatgctgga gcctgcccat tgcgcggcga tgcggcgcgt cggacaaggt
     3661 ctcgcgggac gctcaagctc gctctgtcct gagggcctcc aacacgtggc gtcgagcgcg
     3721 aaaaacgaac tgctcgctgt ctatctggag cgtcgccccg ctctcatccg ttcgttcgct
     3781 gcccggaccg gatcccagga tcgagccgag gacgtagttc aggacattta tgtccgcctt
     3841 caggccctga cc
//
LOCUS       DASVTM010000135         4662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_13891, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000135 DASVTM010000000
VERSION     DASVTM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13891"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1822)
                     /locus_tag="VF633_03810"
     CDS             complement(20..1822)
                     /locus_tag="VF633_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8470216.1"
                     /translation="MSAVAAEPSTPEDEAHAIMPTAQGAAGDSASPEAIRRLSRALAG
                     DRTSVKAQRKAIETLKAALASVRVGDYAGGANRALQVLKVDERNGLAWHVLAICQEKA
                     GQLAPALTSYDAALRLMPEDANIAHDLGRLAQRLGYLEIAEKLMLKFLAAEPGHVEGT
                     NNLACVLRDEKRYDEAIDLLRNLIQIEPAEPTLWNTLGTVLSDQGQMAQSLTFFEEAL
                     RLDPAFAKARYNRANVRQPLGDAAGALEDLDHAIPGAETPYEKAMMNMARALTLLGLG
                     RLEEGFEAYEVRLNPDLTEAMGVAIDCARWNPKTEDIAGKRLLVVGEQGIADEMLFGT
                     ALPDVIEAVGPTGKVFIAVEGRLVDLFQRTFPTAVVGGHRAVRLEGRLTRYVPFMEQI
                     AEAEGSADRWVPMASLSAVYRKDLSAFPDRDGYLVADPVKIARWKAELDALGPGLKVG
                     LHWKSSVLTGVRARYFSDFERWAPVLTAPGCVMVNLQCGDVSTDLAAAEAAGANIWTP
                     PMNLKDDLDDLAALSNALDLVIGPGIAGTNMAASTGARTWLIHAPDDWHLLATDHYPF
                     YPRVRTFATGGFDGWPRVIAEVRAALDAAVKSGF"
     gene            1960..2382
                     /gene="flbT"
                     /locus_tag="VF633_03815"
     CDS             1960..2382
                     /gene="flbT"
                     /locus_tag="VF633_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEX8470217.1"
                     /translation="MPLKLSLKPGEKFVLNGAVVQNGDRRGVLILQNKASVLREKDIM
                     QLEEVTTPARRIYFPVMMMYLDETSASKVYDEFVRRLTEFMGATRNPQVMQDCVDASK
                     HVMAREYYKALMAARKLVDYEEQRLGNAAGVQTGGDAD"
     gene            2396..2707
                     /gene="flaF"
                     /locus_tag="VF633_03820"
     CDS             2396..2707
                     /gene="flaF"
                     /locus_tag="VF633_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEX8470218.1"
                     /translation="MEYRLFGQVTRALMHAATVDPSDTKTRIDALDWNRRLWSTLATD
                     CADPGNAMSMPLRAQIISISIFVGKHSSRVMRNEDDFETLIDINRSIMQGLSQGVQQA
                     A"
     gene            2911..3738
                     /locus_tag="VF633_03825"
     CDS             2911..3738
                     /locus_tag="VF633_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX8470219.1"
                     /translation="MANSVNTNSGALIALQNLNATNAQLAQTQTRINTGKKVSSAKDN
                     GAIWAIAQGQRADIGALGAVKSSLDRGQSAVDVSLAAGESVSDLLLQMKEKALAATDK
                     SLSTASRSALNEDFKAIRDQITTVTNNADFNGINLIKVGATGFQALANASGTATLTVK
                     PEVLALGSTNVTVTATTTIGTSTLATTALGLVNASIDRVSGALARLGTSSKAVTTHAT
                     FVGKLADALESGVGNLVDADLAKESATLQALQTKQQLGVQALGIANQAPQILMSLFQ"
     gene            complement(4212..>4662)
                     /locus_tag="VF633_03830"
     CDS             complement(4212..>4662)
                     /locus_tag="VF633_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX8470220.1"
                     /translation="WVLTREFRMGFSKTAPRYVFVPKVRRLVRRRPRGVNVLKTLRGV
                     ENLTRPRLSVLTGPISEVLMGTTCVLLALVMALPIPFGDMLPGIGLVFFALGVMQKDG
                     VAVLIGVLGSIATAGYLILVWRTAIEAAHHAAGWVAHLAGWFAGLFG"
BASE COUNT          894 a         1468 c         1495 g          805 t
ORIGIN      
        1 gcctagcgga gccgttccgt cagaagccgg acttaaccgc cgcatcaagc gcagcacgga
       61 cttcagcaat gacacgaggc cagccgtcga aaccaccggt cgcgaaggtg cgaacgcgcg
      121 gatagaaggg atagtgatcg gtcgccagca ggtgccagtc gtccggcgcg tggatcagcc
      181 aggtgcgtgc gccggtcgag gccgccatat tggtgccggc gatgcccggg ccgatgacga
      241 gatcgagggc gttcgacagg gcggccagat cgtcgagatc gtccttgaga ttcatcggcg
      301 gcgtccagat attcgcaccc gcagcctcag ccgccgcgag atcggtcgag acgtcgccac
      361 actgaagatt gaccatgacg caacccggcg cggtcaacac cggagcccag cgctcgaagt
      421 ccgagaaata acgggcgcgg acgccggtca ggaccgagct cttccaatgc agaccgacct
      481 tgaggccggg gccgagcgca tccagttccg ccttccagcg cgcgatctta accgggtcgg
      541 cgaccagata gccgtcccgg tccggaaagg cggacaggtc cttgcggtac acggccgaca
      601 ggctggccat gggaacccag cggtcggcgc tgccttcggc ttccgcaatc tgctccatga
      661 aggggacgta gcgggtcaac cggccttcta gacgcacggc ccggtgtccg ccgacgacag
      721 cagtcgggaa ggtgcgctga aacaggtcca cgaggcggcc ctcgaccgcg atgaagacct
      781 tgccggtcgg tccgacagcc tcgatcacgt ccggcaaggc tgtgccgaac agcatctcgt
      841 cggcgatgcc ctgttcgccg accaccagca gccgcttgcc ggcgatgtcc tcggtcttcg
      901 gattccagcg cgcgcagtcg atggcgacgc ccatagcctc ggtcaggtcc gggttcagac
      961 ggacctcata ggcttcgaat ccttcctcaa ggcgcccgag gcccagcagg gtcagcgccc
     1021 gggccatgtt catcatggcc ttttcgtagg gggtttcggc accgggaatg gcgtggtcga
     1081 ggtcttcgag cgcgcctgca gcgtcgccga gcggctggcg cacgttggcg cggttgtaac
     1141 gagccttggc gaaggccggg tcgagacgaa gagcctcttc gaagaaggtc agggactggg
     1201 ccatctgacc ctggtcgctg agcaccgtgc cgagggtgtt ccacagcgtc ggctcggcag
     1261 gttcgatctg gatgaggttc cgaagaagat cgatcgcctc gtcgtagcgt ttctcatcac
     1321 gcaggacgca ggcgagattg ttggtcccct cgacgtggcc gggctcggcg gcgaggaatt
     1381 tcagcatcaa tttctcggcg atctcaaggt agccgagacg ctgggccaga cgaccgaggt
     1441 cgtgggcgat gttggcgtcc tcgggcatca gcctcagcgc cgcgtcatag gaggtgagcg
     1501 cgggagcgag ctggcccgct ttctcctgac agatcgccag cacatgccag gccaggccgt
     1561 tgcgctcgtc gaccttcaac acctgcagcg cgcgattggc gccgccggcg taatcaccca
     1621 cccgcaccga ggcgagggcc gccttaagcg tctcgatcgc cttgcgctgg gccttgacgg
     1681 aggtgcggtc cccggcgagg gctctggaca gacggcggat agcctctggc gacgccgagt
     1741 cgcccgccgc accctgcgcc gtcggcatga tggcgtgagc ttcgtcctcg ggagtcgatg
     1801 gttctgcggc gactgcggac atctgcgaaa aggctctaat ggcttgaatg tcctgattgc
     1861 cgcgaaccct cacaatggat ggttaaccac gcgcgtttaa cctgcgtttg ccggctcttg
     1921 ttatggacga tgagcggtca tggatggaga cctcgccgga tgcccttgaa gctgtcgctg
     1981 aaacccggtg agaaattcgt cctgaacggc gcggtcgtcc agaacggcga ccgccggggt
     2041 gtgctgatcc ttcagaacaa ggcgtccgtg ctgcgtgaga aagacatcat gcagcttgag
     2101 gaagtcacca ctcccgctcg acgcatctat ttcccggtca tgatgatgta cctggacgag
     2161 acgtcagcgt ccaaggtcta cgacgaattc gttcgtcgcc tgaccgagtt catgggcgcc
     2221 acacgcaatc cgcaggtgat gcaggactgc gtggacgcgt cgaagcacgt gatggcccgc
     2281 gaatactaca aggccctcat ggccgcgcgg aaactggtag attacgaaga acaaaggctc
     2341 ggtaatgctg caggcgtaca aacaggcggc gacgcggact gaatctccgc gtgagatgga
     2401 gtaccgtcta ttcggccagg tcacgcgcgc tctgatgcac gcggcgaccg tcgatccgtc
     2461 ggacaccaag acacgtatcg atgccctcga ctggaatcgg aggctgtggt cgacgctcgc
     2521 caccgattgc gccgacccag gcaatgccat gagcatgccc ctgcgcgccc agatcatctc
     2581 gatcagtatt tttgtcggca agcactcctc gcgggtaatg cgtaacgagg atgacttcga
     2641 gaccctgatc gacatcaacc gttcgattat gcaaggcctg tcccaaggcg tgcagcaggc
     2701 cgcctgaccc cactcaggtg gtcggcaaat cctgaccggc ggccggtcgt tgatgccggg
     2761 ttaaccatcc ggactttctc taacctgttg aattcatggc ggccagatcc ctagcggtct
     2821 ggccgtcttc ttgcgtgtgc gtgtgcgagc caagaaccgg ctctacggcc agaaggccgt
     2881 tttcccccga ccagaatgga aggacacacc atggccaatt cggtcaacac gaactccggc
     2941 gcgctcattg cgctccagaa cctgaacgcg accaacgccc agctggccca gacccagacc
     3001 cgtatcaata cgggcaaaaa ggtctccagc gccaaggaca acggggcaat ctgggcaatc
     3061 gcccagggcc aacgtgccga catcggggct ttgggagcgg ttaaatcgtc gctcgatcgc
     3121 ggtcagtccg cggtggatgt gtcactggca gcgggcgagt cggtctctga cctcttgctc
     3181 caaatgaagg agaaggcact ggcggcgact gacaagtcgc tgtcgacggc gtcgcgtagc
     3241 gcgctgaacg aggacttcaa ggctatccgc gaccagatca ccacggtgac caacaacgct
     3301 gactttaacg ggatcaacct gatcaaggtc ggcgcaacgg gtttccaggc tctggccaat
     3361 gcgagcggca ctgcgaccct gaccgtcaag ccagaagtcc tggcgctggg gtccacgaac
     3421 gtcaccgtca ccgcgaccac gaccatcgga acctcgaccc tggccaccac ggccctgggt
     3481 ctggtcaacg cctcgatcga cagggtttcc ggcgcgctcg cgcgtctggg cacgtcgtcg
     3541 aaagccgtca cgacccacgc gaccttcgtc gggaaactgg cggatgcgct ggaatcgggc
     3601 gtgggcaatc tggtggacgc cgatctggcg aaggaaagcg ctacgcttca ggcgttgcag
     3661 acgaagcaac aactgggcgt tcaggctctg ggtatcgcca accaggcgcc gcaaatcctc
     3721 atgtctctgt tccagtaaga ccgcgcgttc agggaacaag cgctggagag gggacccctc
     3781 tccagcgctt ccttttttgt aagagggaaa gagcgctagc gctcgccgcg cggcggcttg
     3841 ctgcttgagc gcaaggcgtg gcggccccga tggtcagcga aaaacgcttc acgcccaact
     3901 cttgacgaga gcccaaaccc gtcccacctt cgacatccct gttcaacagt cgaaaggagg
     3961 tgatcaagtg atctcattgt ccccaacccg tacggtgacc gaagcttggt cctctcgcga
     4021 ggtccgggcc tgatcctttc aggcttgctt taggtccggc cgtaacgcgg tcagacaatg
     4081 agggccgtcg gggcaacccg gcggcccttt ttgctggagt ctgtgggcgc tatcgctcgg
     4141 ctcgcggagc ctgctgcatg ttcgcactgc ccggccctat gcctgcgagg ctatcgcttg
     4201 ctgcttgcgc tttacccgaa cagcccggcg aaccagccgg cgaggtgggc gacccagccg
     4261 gcggcgtggt gggcggcttc gatggcggtg cgccagacca ggatcagata accggcggtg
     4321 gcgatcgagc ccagcacgcc gatcaggaca gcgacgccgt ccttctgcat cacaccgagg
     4381 gcgaagaaga ccaggccgat gccggggagc atgtcgccga acgggatggg caaggccatc
     4441 acgagcgcca gcagcacgca ggtggtcccc atcaggactt cgctgatcgg gccggtcagg
     4501 accgacaggc gtgggcgggt gaggttctcg acgcctctga gagtcttcag cacattgacg
     4561 ccccgaggac ggcggcggac gaggcgcctg accttcggga caaacacgta tcgaggcgcc
     4621 gttttcgaga atcccatccg gaactcacgc gtcagaaccc ag
//
LOCUS       DASVTM010000136         5065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_759731,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000136 DASVTM010000000
VERSION     DASVTM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759731"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3007)
                     /locus_tag="VF633_03835"
     CDS             complement(<1..3007)
                     /locus_tag="VF633_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8470221.1"
                     /translation="MKNISSWSIKNPIPIILLFTLLTIAGLLSFSNMRINNNPDIDFP
                     LVAVTAVRPGAAPSEMEIQVTRLIEDSLAGLSGVRHINSQITDGVSSTTIEFELGTDT
                     ERATNDVRNAMSGLRADLPQDMQEPSVQRIDITGDALITWVVRSTTMTPEQISWFVDN
                     DVSRSLLSIKGVGEVNRGGGVDREIAVELDPDRLASYGLTAAAVSQALTSVNADQPGG
                     RVTISGSERSIRTLGAATSIEALRETLVPLSGGRSVRLGDLGRIEDHWSEPRRLARYN
                     GQEAITFNFLRSRTASEVRIAETVREAVAKIDEEHPELTISQVTASVEQIEESYIASL
                     EALLLGAVLAVIVVFIFLRDWRATFITAMAMPLSLIPAFAVLGPLGQSLNVVSLLALS
                     LTIGILVDDAIVEIENIVRHMRDGKPPYDASMEAADEIGLAVVATTATIIAVFAPVGF
                     MPGIIGQFFKAFALAACVSVAFSLVVARTLTPLMAAYLLKHHKQDDKDPFWMARYLKA
                     LSWSLNNRWKVFAIGTALFLATALLIAPRVPFEFQPGGDVGRAGFSVELPPGATLAQT
                     DAVVQRLTRDLRARKEVTSVYASIGGQEVNQANVYADLVDKGDRDLSQQQFARTMVDG
                     WKPIPGARIGAGVAQQGGGPSDGTSYQFAILSDNGAALTAAARKVEAEMRTVPGLANV
                     VNTASIARPEILVTPRPDQAALMGVSTSAISQAVRVATIGDVDQNLPKYNLGDRQVPI
                     RLRLTRDAREDLAVLETLRVPTASGGSVPLSAVADIRFGAGPSQVLRQDRSRVATISA
                     ELDGIRSGDAGAAVNKLPTVMNLPAGVRQVPAGDQEFIQEMISGFAVAFGTGILLMYA
                     VLVLLFRSFAYPLTIMASLPLAIGGAFVALMLGGSSFSISSLIGVLMLMGIAAKNSIL
                     LVDYVIIAEKEGMGRREALMDAAHKRARPILMTTFAMGAGMIPVAVGWGADVEFRQPM
                     AIAVVGGLISSTFLSLLFIPPIFTIVDDIS"
     gene            complement(3018..4163)
                     /locus_tag="VF633_03840"
     CDS             complement(3018..4163)
                     /locus_tag="VF633_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8470222.1"
                     /translation="MATKIMTRSTTLAGLMLTTALVLSACGGGGEDKKATAEKDGPQA
                     NAGQTVTVVTAAVTDVARTVTASGTVSAWEEVPVGAETGGLTATAVYVDEGSYVRQGQ
                     PLVQLNDALLRAQVQQQQAGVQTAEANAARDDAALGRAQELKERGFLSQASLDTALAN
                     QRASRANLSSARASLSETRTRLSQATLRAPVSGLIISRSVTKGQIVAAGTEMFRMVRD
                     GRLELDAQVPETELGLIRAGQSATIASDQVGQATGSVRIVTPEVNAETRLGLARIALS
                     GAGGFRPGMFARARIDVGAQPTVTVPTAAVLYRENRAGVFVLTADNHARFQPVTVLSR
                     SADRTSVSDLAAGSRIVVEGAGFLGDGDRVRVGTAAPVAAPAAPAAR"
     gene            4395..>5065
                     /locus_tag="VF633_03845"
     CDS             4395..>5065
                     /locus_tag="VF633_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8470223.1"
                     /translation="MTTPPISQIRNFSVVAHIDHGKSTLSDRLIQFTGGLTAREMSAQ
                     VLDNMDIEKDRGITIKAQTVRLNYKAKDGLEYVLNLMDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYQAIDNNHEIVPVLNKIDLPAAEPERVRQQIEDVIGIDA
                     SDAVLCSAKSGIGIEDVLEAIVTRLPAPKGDPDAPLKALLVDAWYDPYLGVVVLVRVF
                     DGVLKA"
BASE COUNT          865 a         1598 c         1771 g          831 t
ORIGIN      
        1 cgctgatgtc gtcgacgatg gtgaagatcg gcggaatgaa cagcagagac aggaaggtcg
       61 aggagatcag cccgccgacc acggcgatgg ccatgggttg gcggaactcg acgtcggcgc
      121 cccaacccac ggcgaccggg atcatgccgg cccccatggc gaaggtcgtc ataaggatcg
      181 gccgcgcccg cttgtgggcg gcgtccatca gggcctcgcg gcggcccatg ccttccttct
      241 cggcgatgat cacatagtcg accagcagga tggagttctt ggccgcgatc cccatcagca
      301 tcagcacccc gatcagcgac gagatcgaga agctggagcc gccgagcatc agggccacga
      361 aggcgccgcc gatcgctaac ggcagggagg ccatgatggt gagcggatag gcgaagctgc
      421 ggaacaacag caccaggacg gcatacatca agaggatgcc ggtgccgaag gcgaccgcga
      481 agcccgagat catctcctgg atgaactcct ggtccccggc cgggacctgg cggacgccgg
      541 cgggcagatt catgacagta ggcagcttgt tcaccgcagc tcccgcatcg ccactacgga
      601 tgccgtccag ttcggccgag atggtggcca cgcgcgaacg atcctgacgc aggacctgtg
      661 acggacccgc gccgaaacgg atgtcggcca cggcgctgag cggcaccgag ccgccgctgg
      721 ccgtcgggac gcgcagggtt tccagcacgg ccagatcctc gcgcgcgtca cgggtcaggc
      781 gcagacggat cggcacctga cggtcaccca gattgtattt cggcaggttc tgatcgacgt
      841 cgccgatggt ggcgacacga acggcctgag agatcgcgct ggtcgagacg cccatgagcg
      901 ccgcctgatc ggggcgtggc gtgacgagga tttcggggcg ggcgatcgag gcggtgttga
      961 ccacgtttgc gaggcccgga acggtgcgca tctcggcctc gaccttgcgc gcggccgcag
     1021 tcagggcggc gccgttgtcg ctgaggatag cgaactgata gctggtgccg tcgctggggc
     1081 cgccgccttg ctgtgccacg cctgcgccga tacgggctcc gggaatgggt ttccaaccgt
     1141 cgaccatggt ccgggcgaac tgttgctggc tgaggtcgcg atcgcccttg tcgacgaggt
     1201 cggcgtagac attggcctga ttgacctcct ggccaccgat cgaggcatag accgaggtca
     1261 cctccttgcg ggcgcgcagg tcgcgcgtca ggcgttgaac cacggcgtcg gtctgggcca
     1321 gagtcgcgcc gggcgggagt tcgaccgaga agccggcgcg gccgacgtcg ccgccgggct
     1381 gaaactcgaa cggcacccgg ggcgcgatca gaagggccgt cgcgaggaac agcgccgtgc
     1441 cgatggcgaa caccttccag cggttgttca ggctccagct cagggccttc agataacgag
     1501 ccatccagaa ggggtccttg tcgtcctgct tgtggtgctt gagcaggtag gccgccatca
     1561 gcggcgtcaa cgttcgcgcg accacgaggg agaaggccac agacacacag gccgcgaggg
     1621 cgaaggcctt gaagaactgg ccgatgatcc cgggcatgaa gccgaccggt gcgaagacgg
     1681 cgatgatggt ggcggtggtc gccacgacag ccagaccgat ctcgtcggcg gcctccatcg
     1741 aggcgtcata gggcggtttg ccgtcgcgca tgtggcggac gatgttctcg atctccacga
     1801 tcgcatcgtc gaccagaatg ccgatcgtca gcgacagggc cagcagactg accacgttca
     1861 gcgattggcc cagcggcccg agcacggcga aggcagggat cagcgacagc ggcatggcca
     1921 ttgcggtgat gaaggtggcc cgccagtcgc gcaggaagat gaacaccacg atgacggcca
     1981 aaacggcgcc caacagcaag gcttccagcg aggcgatata gctttcttcg atctgttcga
     2041 cactggcggt gacctggctg attgttagtt ccgggtgctc ctcgtcgatc ttggcgacgg
     2101 cttcgcggac cgtctcggcg atcctgacct cggaagcggt gcgcgagcga aggaagttga
     2161 aggtgatggc ttcctgaccg ttgtagcggg ccagacggcg aggctcggac cagtggtcct
     2221 cgatccggcc aagatcgccc agacgcaccg aacggccgcc ggacaggggg accagggtct
     2281 cgcgcagggc ctcgatggag gtcgcggcgc cgagggtccg gatcgaacgt tccgagccgg
     2341 atatggtcac gcgtccgcca ggctggtcgg cgttgacgct ggtcaacgcc tgactgactg
     2401 cggcggccgt caggccgtag gaggccagac ggtcgggatc gagctcgacc gcgatctcac
     2461 ggtccacgcc accgccgcgg ttgacctcgc ccacgccctt gatcgagagc aggctgcggc
     2521 tgacgtcgtt gtcgacgaac cagctgatct gttccggcgt catggtggtg gagcggacga
     2581 cccaggtgat cagggcgtcg ccggtgatgt cgatgcgctg gacgctgggt tcctgcatgt
     2641 cctggggcag gtctgcgcgc aggccgctca tggcgttgcg cacgtcattg gtggcgcgtt
     2701 cggtatcggt gcccagctcg aactcgatgg ttgtcgagga cacgccgtcg gtgatctggg
     2761 agttgatgtg acgcacgccc gacaggccgg cgagcgagtc ctcgatcagg cgggtgacct
     2821 ggatctccat ctcgctgggc gcggcgcctg ggcggacggc cgtgaccgcc acgagcggga
     2881 agtcgatgtc cgggttgttg ttgatccgca tgttgctgaa gctcagcagc cccgcgatcg
     2941 tcagcagggt gaacagcagg atgatcggga tcgggttctt gatcgaccag gaggagatgt
     3001 tcttcatagt cggatcctca gcgcgcggcc ggagcggcgg gcgccgcgac cggcgccgca
     3061 gttccgacgc gcacacggtc gccgtcgccg aggaagccgg cgccctcgac cacgatccgt
     3121 gaaccggccg ccagatccga cacggacgtc cggtccgctg atcgcgacaa aacggtcacg
     3181 ggctggaacc gggcatggtt gtcggctgtc agaacgaaca cgccggcgcg gttctcacgg
     3241 tacaggacgg cggcggtggg cacggtgacg gtgggttggg cgccgacgtc gatccgggct
     3301 cgtgcgaaca tgccgggacg gaagccgccg gcgccgctga gagcgatgcg cgccagtccc
     3361 agacgggtct cggcgttcac ctcgggcgtc acgatgcgga cgctgccggt ggcctgaccg
     3421 acctgatccg aggcgattgt cgcagactgg ccggcgcgga tcagacccag ttcggtctcg
     3481 ggcacctggg cgtccagctc cagtcgaccg tcgcggacca tacggaacat ttcggtgccg
     3541 gcggccacga tctgaccctt ggtcacgctg cggctgatga tcaggcccga cacgggcgcg
     3601 cgcagggtcg cctgcgacag acgggtgcgg gtctcggaca gcgaggcgcg ggccgacgac
     3661 aggttggcgc gggacgcgcg ctggttggcc agtgcggtgt cgagcgaggc ttgactgagg
     3721 aagcctctct ccttgagttc ctgcgcgcgg ccgagagcgg cgtcgtcacg ggccgcgttg
     3781 gcctccgcgg tctggacgcc cgcctgctgc tgctggacct gggcgcggag cagcgcgtcg
     3841 ttcaactgga ccagcggctg gccctgacgg acatacgaac cttcgtcgac atagacggcg
     3901 gtggcggtca gaccgcctgt ctcggctccg accggaacct cttcccatgc ggacacggtg
     3961 cccgaggcgg tgacggtgcg cgccacatcg gtcacggcgg ccgtgacgac agtcaccgtc
     4021 tggccggcgt tggcctgagg gccatctttc tcagccgtcg ccttcttgtc ctcgccgcca
     4081 ccgccacagg cgctcaacac cagcgccgta gtcagcatca atccggccag ggtggtagag
     4141 cgggtcatga tctttgtcgc cacgttggaa tatcctgtcg catggcccgc ggggaggaac
     4201 gggtcgaggc gccgcgtttg cggcgggaga ttgccgctcg ccaatagccg ttccacggct
     4261 gttctcaaac acccacctgc gccattacgg ggttgtaatc cgtagccgcc ggggttccag
     4321 atcgctggat agcgctggtg gaggccggct ctgcgttggc gttcgggcgg cctccatgct
     4381 aaccgcgccg cacaatgacg acgcccccca tttcccagat ccgcaacttc tccgttgtcg
     4441 cgcatatcga ccacggcaaa tcgacacttt ccgaccggct gattcagttc acgggcgggc
     4501 tgaccgcgcg cgagatgagc gctcaggttc tcgataatat ggatatcgag aaggaccgcg
     4561 ggatcaccat caaggcgcag accgtgcgcc tgaactacaa ggccaaggac gggctggagt
     4621 atgtcctcaa cctgatggat acgccggggc acgtcgactt cgcctatgaa gtcagccgca
     4681 gtctggccgc ttgcgaaggc tcgctgctgg tggtcgatgc gtcgcagggc gttgaggccc
     4741 agaccttggc caatgtttat caagccattg ataataatca tgaaatcgtt cccgtcctca
     4801 acaagatcga tctgcccgcc gctgagcccg agcgcgtcag gcagcagatc gaggacgtca
     4861 tcggcatcga cgcttcggac gccgtcctgt gcagcgccaa gtcgggcatc ggcatcgagg
     4921 acgtgctcga ggccatcgtc acccgcctgc cggcccccaa gggcgatccc gacgcgccgc
     4981 tgaaggcgct gctggtcgac gcctggtacg acccctacct cggcgtcgtc gtgctggtgc
     5041 gcgtgttcga cggcgtgctg aaggc
//
LOCUS       DASVTM010000137         5081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_119078,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000137 DASVTM010000000
VERSION     DASVTM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119078"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VF633_03850"
     CDS             complement(<1..682)
                     /locus_tag="VF633_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX8470224.1"
                     /translation="MPAVVRGGRRQSSSSAPKRGASSSGKKSGGQRGARNAPVVNGKF
                     AAVGKLDLSPRAVVISIVAGVAVLAAVLATGARAERISASATRGMDQITTGMGLSLKR
                     VHITGSSAEATPAIRAALDVRAGQPITSLDLAAMRDRVQGVGFVQEARVVRLLPDTLI
                     VEVKEHDRLAVWQTGGQTYVIDTHGRTIAGADAGRYPQLPLVVGKGADLAAAELLPLL
                     AQRPRLIGR"
     gene            complement(670..1614)
                     /locus_tag="VF633_03855"
     CDS             complement(670..1614)
                     /locus_tag="VF633_03855"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX8470225.1"
                     /translation="MSPSKPFSELHVAVLLGGISAEREVSLVSGQGCADALEGQVAKV
                     SRVDADRDLAAVLTELKPDVVFNALHGAWGEDGCVQGVLETLGIPYTHSGVLTSALAM
                     DKDKSKAVLKAAGVVVPGGGLFSRHEVAAGHVIPPPYVIKPNAEGSSVGVFLVREGAN
                     GPVTEVGEDSWTYGETVMVEPYIPGKELCVSVVGEKSGPRALTVTDITPKKGFYDYEA
                     KYAPGGSVHELPAVLPTHVFEKALRQAELAHVAMGCRGVSRSDFRYDDVKDDLVFLEV
                     NTQPGMTPTSLVPEQAAYVGMSYQDLVRWIVEDASCPR"
     gene            complement(1722..2645)
                     /gene="murB"
                     /locus_tag="VF633_03860"
     CDS             complement(1722..2645)
                     /gene="murB"
                     /locus_tag="VF633_03860"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX8470226.1"
                     /translation="MSVDWREALPSVRGKLLRDEPLGPFTWFRVGGAAEVLFIPADAD
                     DLADFLKALEPDVPVMVLGVGSNVIVRDGGVEGVVIRLAGRPFAGITTTGETVTTGAG
                     ALDAMVAKASAKAGLAGLEFYAGIPGTIGGALTMNAGCYGSETKDILVSAWGLTQAGE
                     RVDYSLADFGYTYRHSEAPADIIWIEAVYRGTPDEPAAVAARIAEITSRRETTQPIRE
                     KTGGSTFKNPPGHSSWKLVDEAGWRGKLHAVTGGGAMFSDLHSNFMINPGEATAADIE
                     GLGEAVRADVLAKTGIQLDWEIKRVGCSGQA"
     gene            complement(2642..3094)
                     /locus_tag="VF633_03865"
     CDS             complement(2642..3094)
                     /locus_tag="VF633_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8470227.1"
                     /translation="MTDVVLTGGCQCGAVRYELSAQPETEFCHCRMCQRAVGGVFAAL
                     SGVKKTEFRWTRGEPSFFASSTLSKRGFCAACGTPLTFAYDAKDGIEVTTGSLDDPEL
                     APIASHYGVESRVSWLKLCDGLPEHRTGESPDSMVHRADFQPKQDADR"
     gene            complement(3091..4533)
                     /gene="murC"
                     /locus_tag="VF633_03870"
     CDS             complement(3091..4533)
                     /gene="murC"
                     /locus_tag="VF633_03870"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX8470228.1"
                     /translation="MIARLRPVPFDLGPVHFVGIGGIGMSGIAEIMLKIGYAVQGSDA
                     KASANTERLAALGAKIFIGHDAAHVSEGVSAVVYSTAVKATNPEMMTARERRIPLVRR
                     AEMLAELMRLQFSIAVGGTHGKTTTTSMVACLLDAAGFDPTVVNGGIINAYGTNAKVG
                     DGDWIVVEADESDGSFLRLKSTVAIVTNIDPEHLDHYGDFDAVRKAFVDFVENIPFYG
                     FAAVCLDHPEVQRLVASIDNRRLVTYGMNPQAQVRAENVAMGPDGCRFDVIFQSGDPA
                     LEPTRIDGLHLPMAGGHNVANALAAIAVARELDVSDDAIKSGLAAFGGVKRRFTTTGV
                     VNGVRIVDDYGHHPVEIAAVLKAARQVVQSSEGGGGKVIAVMQPHRYTRLRDLMEEFS
                     TSFSDADTVLIADVYPAGEAPIEGVDKQALVEGVRRYGHRHVSALENVTTLPRVIAEV
                     ATSGDVVVLLGAGDITSWAYALPAQLEALA"
     gene            complement(4702..>5081)
                     /locus_tag="VF633_03875"
     CDS             complement(4702..>5081)
                     /locus_tag="VF633_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX8470229.1"
                     /translation="ANYIYSILTGYHTPPAGLRMTSTQHYNAYMPGDLKPFMPEGAET
                     PPGGFIAMPPPLAQDGLVTYDDGTAATTDQMAKDVSAYIAWASEPHAVERKQAGMGVL
                     VFLALFAGVTFMSYRRIWKNVAH"
BASE COUNT          908 a         1628 c         1718 g          827 t
ORIGIN      
        1 cgcggccaat cagacgcggg cgctgggcca gcagcggcag cagctcggcg gcggccagat
       61 cggcgccttt tccgaccacg agcggaagct gcggataacg cccggcgtct gcgccggcga
      121 tggtccggcc atgagtgtcg atgacgtagg tctggccgcc ggtctgccag acggcgagac
      181 ggtcgtgttc cttgacctcg acgatcaggg tgtcaggcag caggcggacg acgcgcgcct
      241 cctgaacgaa gcccacgccc tgaacccggt cgcgcatggc ggcgaggtcg aggctggtga
      301 tcggttgccc ggcacgcacg tcgagggcgg cgcggattgc aggcgtcgcc tctgccgagg
      361 agccggtgat gtggacgcgc ttcagcgaca gacccatgcc ggtcgtgatc tgatccatgc
      421 cgcgagtcgc ggacgcgctg atacgctcgg cgcgcgcacc ggtggccaga acggccgcca
      481 gcacggcgac gcctgcgacg atggagatga cgactgcgcg aggcgacaga tcgagcttgc
      541 cgacggcggc gaatttgccg ttgacgacgg gagcgttacg agcgccgcgc tggcccccgc
      601 tcttctttcc agaagacgag gcgccgcgtt tgggagccga actcgaactc tgtcgccgac
      661 cgccgcgtac taccgcgggc atgaggcgtc ctccacgatc caccggacaa ggtcttgata
      721 ggacatcccg acataggccg cctgctctgg aaccagagat gtcggggtca tgccgggctg
      781 cgtgttgacc tccagaaaga ccaaatcgtc cttaacatcg tcataacgaa agtcgcttct
      841 cgacaccccg cgacagccca tggcgacatg cgccaactcg gcctgacgca gggctttctc
      901 gaacacgtgg gtaggcagaa ccgcaggcag ctcatggaca gagccgcccg gcgcgtattt
      961 ggcttcgtag tcgtagaagc ctttttttgg cgtgatgtcg gtcacggtca gggcgcgcgg
     1021 gccggacttt tccccaacta cggagacgca caactctttg ccggggatgt agggctcaac
     1081 catcacggtc tcgccgtagg tccagctgtc ctcgccgacc tcggtcaccg ggccattggc
     1141 gccttcacga acgaggaaga ccccgaccga agatccctcg gcgttgggct taatgacata
     1201 ggggggcgga atgacgtggc cggcggcgac ttcgtggcga ctgaacaggc cgccgccggg
     1261 aacgacgacg cctgctgcct tcagcacggc cttggacttg tccttgtcca tcgcgagcgc
     1321 cgaggtgaga acgccggagt gagtgtaggg aatgccgagc gtttcgagca cgccctgaac
     1381 acagccgtcc tcgccccacg cgccgtgcag ggcgttgaag acgacgtcgg gtttgagttc
     1441 ggtcaggacg gcggcgaggt cacggtctgc atcgacacgg ctgaccttgg cgacctgtcc
     1501 ctcaagcgcg tcggcgcaac cctggccgga gaccagcgac acctcccgct cggcagagat
     1561 gccgcccagc aggacggcga catgcagttc ggagaagggt ttggaaggcg acatcgaaag
     1621 ctccgggcgt cagaactcaa tgcgcgaacg tccttaacac ctcgtcaatg atgatgtcgg
     1681 cgagaggcta aagaccaaaa aggacgacaa tacctaccgg ctcaagcttg gccggagcac
     1741 ccgacccgct tgatctccca gtcaagctgg atgccggtct tcgcgaggac atcggcacgg
     1801 accgcctcgc caaggccttc gatgtcggca gcggtcgctt ccccgggatt gatcatgaag
     1861 ttggagtgga ggtcggagaa catcgcccct cccccggtca cggcatgcag ctttccgcgc
     1921 cagccggctt cgtcgaccag cttccacgag gaatggccgg gagggttctt gaaggtcgag
     1981 ccgccggtct tttcgcggat gggctgggtg gtctcgcggc gagaggtaat ctcggcgatg
     2041 cgagcggcta cggcggcggg ttcatccggc gtgccgcgat agacggcttc gatccagatg
     2101 atgtcagctg gcgcctctga gtggcggtag gtgtagccga agtcggcgag cgaataatcg
     2161 acccgttccc ctgcctgcgt cagcccccag gccgaaacaa ggatgtcctt cgtttccgag
     2221 ccgtagcagc cggcgttcat ggtcagggcg ccgccgatgg tgccgggaat gcccgcatag
     2281 aactccagcc cggccagccc ggccttggcc gaagccttgg cgaccatggc gtcgagcgcg
     2341 cccgcgcccg tcgtgacggt ttcgccggtc gtcgtgatgc ccgcaaacgg acgcccggcc
     2401 aggcgaatga ccacgccctc aacgcctcca tcgcgcacga tgacgttgga cccgacaccc
     2461 agcaccatga ccggcacatc cggttcgagc gctttcagga aatcggcgag gtcatcggcg
     2521 tccgccggaa tgaacagcac ctcggccgcg ccaccgacgc ggaaccaggt aaagggtccc
     2581 agcggttcgt cgcgcagcag cttgccgcgc acggaaggaa gggcttcgcg ccagtcaacg
     2641 ctcaccggtc ggcgtcctgc ttcggctgga agtcggcacg gtggaccatc gagtccgggc
     2701 tttcgccggt gcggtgttcc ggcaggccgt cgcagagctt gagccaagac acccgggact
     2761 ccacgccgta gtgcgacgcg atcggcgcca gctccgggtc gtcgagactt ccggtcgtga
     2821 cctcgatgcc gtcctttgcg tcataggcga aggtcagcgg cgtaccgcag gcggcgcaga
     2881 agccgcgctt cgacagggtc gaactggcga agaagctcgg ctcgcccctg gtccagcgga
     2941 actcggtctt cttgacgccg gacagggcgg cgaatacccc gccgaccgcc cgttggcaca
     3001 tgcggcagtg gcagaattcg gtctcgggct gggcagacag ttcgtatcga acagcgccgc
     3061 actggcaacc gccggtcagg accacgtccg tcatgccagg gcctcgagct gcgccggcag
     3121 ggcgtaggcc cagctggtga tgtctccggc gccgagcagg acgactacgt cgcccgaggt
     3181 cgcgacctcg gcgatgacgc gcggcaaggt ggtgacattc tcgagcgccg aaacatggcg
     3241 atgcccgtaa cgacggacac cttcgaccag agcctgtttg tccacgccct cgatcggcgc
     3301 ctcgcccgcc ggatagacgt cggcgatcag aacggtgtcg gcgtcggaga agctggtcga
     3361 gaactcttcc atcaggtcgc gcaggcgggt gtagcggtgc ggctgcatga cggcgatgac
     3421 cttgccgccg ccaccctcgc tggactggac gacctgacgc gcggccttca gcaccgcggc
     3481 gatctcgacg gggtggtggc catagtcgtc gacgatgcgg acgccattga cgacgccggt
     3541 cgtggtgaag cggcgcttga ccccgccaaa ggccgcaaga ccggatttga tggcgtcgtc
     3601 cgaaacgtcc agctcacgcg ccacggctat cgcggccaag gcattggcga cgttgtggcc
     3661 acccgccatc ggcagatgca acccgtcgat gcgcgtcggc tccagcgccg gatcgcccga
     3721 ctggaagatc acgtcgaagc ggcagccgtc cggccccatc gcgacgttct cggcacggac
     3781 ctgagcctga gggttcatgc cgtaggtgac cagacggcgg ttatcgatcg aggccaccag
     3841 ccgctgcacc tcgggatggt cgaggcaaac ggcggcgaaa ccgtagaagg ggatgttctc
     3901 gacgaagtcc acaaacgcct tccgcaccgc gtcgaagtcg ccgtagtgat cgaggtgttc
     3961 ggggtcgatg ttggtgacaa tggccacggt ggacttgagg cgcaggaagc tgccgtccga
     4021 ctcgtcggcc tcgaccacga tccagtcgcc gtcaccgacc ttggcgttgg tgccataggc
     4081 gttgatgatg ccgccgttga cgaccgtcgg atcgaagccc gcagcgtcca gcaggcaggc
     4141 gaccatcgat gtcgtcgtcg tcttgccgtg ggtgccgccc accgcaatcg agaactgcag
     4201 ccgcatcagt tcggccagca tctcggcgcg acgcaccagc ggaatgcgac gctcgcgtgc
     4261 cgtcatcatc tcgggattgg tcgccttgac cgccgtcgaa tagaccacgg cggagacgcc
     4321 ctcgcttacg tgagcggcgt catggccgat gaagatcttc gcacccaagg ccgcaagccg
     4381 ttcggtattg gcgctggcct tggcgtcgga gccctgaacg gcatagccga tcttcagcat
     4441 gatctcggcg atgccgctca tgcctatgcc gccgataccg acgaagtgga cgggaccgag
     4501 gtcgaacgga acggggcgaa ggcgagcgat catccaagcg cattagcaag ggtgggatcg
     4561 gattgcgaga ccgcttccat caggtgctat tgctcgcgct gcgctcaagc agtgagccgc
     4621 cacatcgcga gccatagcgc aaaagaaagc ccgccggagc gaactccggc gggctggatc
     4681 gtttcgcgag gaaggcgcgg cctagtgggc gacgttcttc cagatgcgac ggtagctcat
     4741 gaaggtcacg cccgcgaaca gggcgaggaa aacgagcacg cccatgcccg cttgcttgcg
     4801 ctcgaccgcg tggggctcgg aggcccaggc gatataggcc gagacgtcct tcgccatctg
     4861 gtccgtggtg gcggcggtgc cgtcatcgta ggtgacgagg ccgtcctggg ccaacggcgg
     4921 cggcatggcg atgaagccgc ccggaggggt ctcggcgcct tccggcataa agggcttcag
     4981 gtcgcccggc atataggcgt tgtagtgctg ggtgctggtc atccgcaggc cggcgggcgg
     5041 cgtgtggtaa ccggtcagga tcgaatagat gtagttcgca c
//
LOCUS       DASVTM010000138         8887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_172774,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000138 DASVTM010000000
VERSION     DASVTM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172774"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VF633_03880"
     CDS             complement(<1..230)
                     /locus_tag="VF633_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX8470230.1"
                     /translation="MTSKLIDHSVPLIRAIVIHPDGRSESSRVAEERLEEAAGLARAL
                     DLDVRAEEIVRIRKVTPATLFGAGKVEEIAALV"
     gene            complement(381..626)
                     /gene="hfq"
                     /locus_tag="VF633_03885"
     CDS             complement(381..626)
                     /gene="hfq"
                     /locus_tag="VF633_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX8470231.1"
                     /translation="MSQDKRQNLQDTFLNSVRKTKTPLTIFLVNGVKLQGIVTWFDNF
                     CVLLRRDGQSQLVYKHAISTIMPSAPVQLYEPDADED"
     gene            complement(755..1618)
                     /locus_tag="VF633_03890"
     CDS             complement(755..1618)
                     /locus_tag="VF633_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEX8470232.1"
                     /translation="MSRVAYVNGQYQPHGEATVHVEDRGFQFADGVYEVWAVFDGRLA
                     DFDGHITRLVRSLTELRIDVPMTKGALTRVLKETVRRNRVRNGIVYLQITRGTARRDH
                     PFPTQGTPPSIVVTSHSRNLAQSDAQAAKGVAVITVPDNRWGRCDIKTVGLLPNVLAK
                     QAARDRGAYEAWFVDDMGLVTEGASTNAWIVDADGKLRTRDTQANILNGITRTAILGL
                     IAAEGVELDERPFSVEEAKAAGEAFFTSAGAFVMPAVSIDGARIGDGKPGPIATRLRT
                     LYLEAALRDAI"
     gene            complement(1618..3027)
                     /locus_tag="VF633_03895"
     CDS             complement(1618..3027)
                     /locus_tag="VF633_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8470233.1"
                     /translation="MRSTGADILIVDDEADIRELVSGLLEDEGHSVRVAANAEEALAG
                     IRARKPTLLVLDIWMQGGGMDGLELLDMVKALDADLPVVMISGHGNIETAVSALKRGA
                     YDFIEKPFKSDRLVVVIERALEAASLKRENRRLRAQASVPTGLIGRSAAAQALRTTIS
                     KVAPANSRVLISGPPGSGKELVARQIHEASPRARGEFVAISAAGMTPERLDLELFGEE
                     GADGRPRKIGVFERAHNGTLYLDDVGDMPRESQSRILRVLVDQRFRRVGGEQDVQVDV
                     RVVTSTSRDLKIEITEGRFREDLFHRLNVVPIRVPPLSERREDISELVEYFIDTLSSS
                     QGLPRRRMGDDAVAVLQVHPWPGNVRQLRNNVERLLILATGDLNDPISAEMLPQEVAS
                     SNSGASMGAERTIALPLREAREVFEREYLAAQIMRFGGNISRTAAFIGMERSALHRKL
                     KSLGVSPSRGGEDEEGSLD"
     gene            complement(3037..5310)
                     /locus_tag="VF633_03900"
     CDS             complement(3037..5310)
                     /locus_tag="VF633_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX8470234.1"
                     /translation="MTDTPASPGAQLEALNIDAEPSLWRRFADGRARALFGAAYSIAV
                     VTAAAAIWLVAVAPGAGADGGARAVAGQAVLYILLANLILIGGLALIVGAQVMRLARA
                     QRSDPGARLHLRFVALFSLVAVVPAILIALVFGVLVNRGVDQWFSQNVQDAVENGADI
                     GKAYIGDVGGGMTRDLGAMSNELGGVRQVFDNRIQFTGALTQIAEFFGYPAVYILNGD
                     GEILARGETADAPPYVAPPRSALEIAAEGSEAPVEVTENPDTVRALYPLPDYGDAYLY
                     VVRPLAPGLVSQMRTADQSIQGYREAEDSRARIQTAFALSYLETALLVLVGAVWLGMS
                     AASAISAPIGRLVKAADQVAGGDLSARVDNVGAPGEIADLSDAFNRMTADLQHQQAAL
                     KTASEDAQSRTLFIETVLSGVSAGVIGLDKRGRVSAINDSALQLLSVAEAEVHGQPLA
                     KVAPELSELVARVEAHIEEDIDVSREGETRRLRVRIEGGQGGEMVLTFDDITRLVTAQ
                     RNAAWRDVARRIAHEIKNPLTPIQLSAERLRRKYRSRIGDDVEVFDRCTDTIIRQVGD
                     IGRMVDEFSSFARMPTPRFRTEDPAEVLRQVVFAQRVAAPDIVVELAEPLPEAKFQAD
                     GRMVGQALANILKNAGEAVAARRINTPADETAPAIIAKLVVENGSATFVIEDDGKGLP
                     ARDRDRLTEPYVTTREKGTGLGLAIVKRICEDHGGELKLADAETLHGARVCLIFPLNP
                     NPKVTEAARASGVMAAE"
     gene            complement(5307..6407)
                     /gene="dusB"
                     /locus_tag="VF633_03905"
     CDS             complement(5307..6407)
                     /gene="dusB"
                     /locus_tag="VF633_03905"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX8470235.1"
                     /translation="MADSLQNPAHLGPHPVPRPSLQIGDVTIGGRVLMAPMTGITDLP
                     FRLLASKLGAAYVATEMVASAELARGRPDVVRRAAVGAGLPLTVIQLVGRDPDAMAQG
                     ARMAEAAGADIIDLNFGCPAKEVTGSASGSALMRTPELASRLIAAAVGAVSRPVTVKM
                     RLGWDDAHRNAVDIARRAEDLGVAAVTVHGRTRQQFYTGVADWDAIGEVKAALSIPVI
                     VNGDIITADQAREALSRSGADGLMLGRGVYGRPWLAAHLEQALIDGTELQEPGPEERL
                     AIVSEHLRASVAFYDHPIGLKMFRKHLGWYIEQAPWPVDPLERRQAKSRLCRIDDPEV
                     VIRELKHVWLPLAHFSNSPVEFSPQLVEADAA"
     gene            6472..7632
                     /locus_tag="VF633_03910"
     CDS             6472..7632
                     /locus_tag="VF633_03910"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX8470236.1"
                     /translation="MTFASIVVAAGTGSRAANDVRIAKQWRTLGGKPVLRWSVEALLN
                     AGAEEVVVAVSADGHEAAAAALAGLNGWRSVTGGETRAESVRAGLAALTGPDDQILLV
                     HDAARPLLSDSVIQRLLMALETADGALPALPVSDTLKKTSGDAITGTVDRDGLWRAQT
                     PQAFRKDRLLAAWANWPVSETPTDDCAVVERDGGKVVVVEGEARLMKLTFPEDFAMAE
                     ALIAAGTRMQTRVGSGFDAHRWGPGGSVWLCGVEIPHDQTLIGHSDADAGLHALTDAI
                     LGALGDGDIGDHFPPTDPKWKGASSDLFLSYAADKVAARGGRMVHVDVTLICERPKVK
                     PHRQAMRERVAALLSLPLDAVSVKATTTEAMGFTGRGEGLAAQAIATIELPA"
     gene            complement(7629..>8887)
                     /locus_tag="VF633_03915"
     CDS             complement(7629..>8887)
                     /locus_tag="VF633_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX8470237.1"
                     /translation="EFYLLMSLGGVVGGAFTALLAPVIFTTVLEYPLVLVLVGLARPL
                     SKAPIHKWEVYAFVVAVLICASPLVLKSVFDADWDLAWAWSRNISASMEQTTQLILLF
                     AMIAAFLIRDRTPAFVIICALIAMTTLSIGRGYDWALTERSFFGVMRVANTRDDRLGG
                     DVHVLMHGTTLHGAQPRDPRFACMPTLYYAPSTPIGQTTQVVQRRAPGVRMGVVGQGS
                     GAMAGYKRGDDRLTFFEIDPMVDRLSRDPQWFTFINGCAQNDGSGGAIDTVLGDARLT
                     MAKQPAGTYDLLLIDAFSSDAVPTHLLTEEAIAGYLKLLKPTGVVILHLSNRNLDIVR
                     PTQAAAVALNAPYLHQLYAEDRNTPDMAEASTEVIILSPTPEGLAEFRADPRWSTLPD
                     TTVRPWTDDYVNLFGALWRQIRRTPY"
BASE COUNT         1520 a         2748 c         3104 g         1515 t
ORIGIN      
        1 accagagccg cgatctcctc gaccttgcct gcgccgaaca gtgtcgcggg cgtcaccttg
       61 cggatgcgaa cgatttcttc ggcgcgaacg tcaaggtcca gcgcgcgggc caagcccgca
      121 gcctcttcca gccgttcctc cgccactcgc gagctttcgc tgcgtccgtc gggatggatg
      181 acgattgcgc ggatcagggg aacggaatgg tcgatcagtt ttgaggtcaa agggtctctg
      241 tcgtcttggg ttcagtgacg gaggcgagcg cacaggccgt catccccgga gaacgccggg
      301 gatgtggatg tagtgcgggt ggccgtattc cgaaagaacc agccgccaaa aagccgcttc
      361 taccaaacga aatgtaggac ctaatcctcg tcggcgtccg gttcgtacag ctgcaccgga
      421 gccgaaggca tgatggtcga gatggcgtgt ttgtagacga gctgcgactg gccgtcgcga
      481 cggagcagca cgcagaaatt gtcgaaccag gtgacgatgc cctgaagttt gacgccattg
      541 acgaggaaga tcgtcagggg ggtcttggtc ttacggaccg agttgaggaa ggtgtcctga
      601 aggttctgac gcttgtcttg cgacatggca ggtttgtcct gttcttgctt gttacgcccg
      661 tcggggagaa cggggaccgg atcgattgcc cggcccgtcc accttagact tgagcgtccc
      721 ggggctgcaa ccgcctcggc agcatatgag acgcttaaat ggcgtcgcgc agggcggctt
      781 cgagataaag cgtccgcagt ctggtcgcga tcgggcccgg tttgccgtcg ccgatccttg
      841 cgccgtcaat ggacacggca ggcatgacga aggcgccggc cgaggtgaag aaggcttcgc
      901 cggcggcctt ggcttcttcg acgctgaagg ggcgttcgtc gagctcgacg ccctcggcgg
      961 cgatcaggcc gaggattgcg gtgcgagtga tgccgttcag gatattggcc tgggtgtcgc
     1021 gggtacgcag cttgccgtcc gcatcgacga tccaggcgtt ggtcgatgcc ccctcggtca
     1081 ccagacccat gtcatcgacg aaccacgcct cataggctcc gcgatcgcgg gcggcctgct
     1141 tggccagaac gttgggcagc agacccaccg tcttgatgtc gcaacggccc cagcggttgt
     1201 cgggcaccgt gatgacggcg acacccttgg cggcttgggc gtcggactgg gccaggttgc
     1261 gggagtgcga ggtgaccacg atgctgggcg gcgtgccttg cgtcgggaac gggtgatcgc
     1321 ggcgagccgt gccgcgcgtg atctgaaggt agacgatgcc gttgcggacg cggttgcgac
     1381 ggacggtttc cttgagcacg cgggtaagtg cacccttggt catcggcaca tcgatgcgca
     1441 gttcggtcag actgcggacc agccgcgtta tatggccatc gaagtcggcg agccggccgt
     1501 cgaacaccgc ccagacctca tagacgccgt cggcgaattg aaagccacga tcctcgacgt
     1561 ggaccgtcgc ctcgccgtgc ggctggtact gaccgttgac gtaggcgacg cgtgacatca
     1621 gtcgaggctg ccttcttcgt cttcgccgcc acgcgacggg gacacgccga gcgacttgag
     1681 cttcctgtgc agggccgagc gttccatgcc gatgaaggcg gcagtgcgcg agatattgcc
     1741 gccgaagcgc atgatctggg ccgccagata ttcgcgttcg aacacctcgc gggcctcacg
     1801 cagcggcagg gcgatggtgc gttcggcgcc cattgaggcg cccgagttgg aagaggcgac
     1861 ttcctgcggc agcatctcgg cgctgatcgg gtcgttcaga tcgcccgttg cgaggatcaa
     1921 caggcgctcg acgttgttgc gcagctgacg gacgttgccg ggccaggggt ggacctgcag
     1981 cacggcgacg gcgtcgtcac ccatgcggcg gcggggcaga ccttgcgagc tcgacagggt
     2041 atcgatgaaa tactcgacca gttcggagat gtcctcgcgg cgttccgaca gcggcgggac
     2101 gcggatcggc accacgttca ggcggtggaa caggtcctcg cggaaacggc cttcagtgat
     2161 ctcgatcttg aggtcgcgcg aggtcgaggt cacgacgcgg acatcgacct gaacgtcctg
     2221 ctcgccgccg acccggcgga agcgttgatc gaccagcacg cgcaggatgc ggctctggct
     2281 ttcgcgcggc atgtcgccga cgtcgtccag atagagggtg ccgttgtggg cgcgttcgaa
     2341 gacgccgatc ttgcggggac ggccatccgc accctcttca ccgaacagtt caaggtcgag
     2401 gcgttcgggg gtcatgccgg cggcggagat ggcgacgaac tcgccccggg cgcgcgggct
     2461 ggcctcgtga atctggcgcg ccaccagttc cttgccggaa ccgggagggc cggaaatcag
     2521 aacgcgactg ttggctgggg cgaccttgga gatggtggtg cgcaaggctt gcgccgcagc
     2581 ggagcgaccg atcagtccgg tcggcaccga agcctgggcg cgcaggcggc ggttctcgcg
     2641 tttcagcgag gcggcttcga gggcgcgttc gatgaccacg accagacgat cggacttgaa
     2701 tggcttttcg atgaagtcgt aggcgccgcg cttcagggcg ctgacggcgg tttcgatatt
     2761 gccgtggccc gagatcatca ccaccggcag atcggcgtcg agcgccttga ccatgtcgag
     2821 cagttcgagc ccgtccatgc ccccgccctg catccagatg tcgagcacca acagggtcgg
     2881 cttgcgcgcc cggatacccg cgagggcctc ctcggcgttg gcggcgacgc gcacggaatg
     2941 gccctcgtcc tcgagcaggc ccgagaccag ttcacgaatg tccgcctcgt cgtcgacgat
     3001 caggatgtca gcgccagtgg aacgcatatc gcctcgctat tcggccgcca tcacaccaga
     3061 cgctcgcgcg gcttcggtga ccttgggatt ggggttgagg ggaaagatca gacagacccg
     3121 cgcgccatgc agggtctcgg cgtcagcaag tttcagctcg ccgccatggt cttcgcagat
     3181 acgcttgacg atggcgaggc cgaggccggt gcccttctcg cgggtggtga cgtagggctc
     3241 ggtcaggcgg tctcggtcgc gggccgggag acccttgccg tcgtcctcga tgacgaaggt
     3301 cgcgctgccg ttctcgacaa ccagtttggc gatgatcgca ggcgcagtct cgtccgccgg
     3361 ggtgttgatg cgacgggcgg cgacggcttc cccggcattc ttgaggatgt tggcaagggc
     3421 ttggccgacc atgcggccgt cggcctgaaa tttcgcttcg ggcaggggct cggctagttc
     3481 gacaacgatg tccggcgctg cgactcgttg cgcgaagacc acctgacgca ggacctcggc
     3541 agggtcttcg gtcctgaagc gtggcgtcgg catgcgggcg aaggacgaga actcgtccac
     3601 catccggccg atgtcgccga cctgacgaat gatggtgtcg gtgcagcgat cgaaaacctc
     3661 gacgtcgtcg ccgatgcggg agcgatattt acgccgcagc cgttcagccg agagctggat
     3721 cggggtcaga gggttcttga tctcgtgagc aatgcgacgg gcgacgtcac gccatgcggc
     3781 gttgcgctga gcggtgacga ggcgggtgat atcgtcgaag gtcaggacca tctcgccgcc
     3841 ctgcccgccc tcgatacgga cgcgcaagcg acgggtttcg ccttcacgcg acacgtcgat
     3901 gtcctcctcg atatgggcct cgacgcgagc gaccagttcg gacagttcag gagcgacctt
     3961 ggccagaggc tggccgtgca cttcggcctc ggccacggac agaagttgca gggcgctgtc
     4021 gttgatcgcg gaaacccggc cgcgtttgtc gaggccgatg acaccggcgc tgacgcccga
     4081 caggacggtc tcgatgaaca gggtgcggga ctgggcgtcc tcgctggcgg tcttcagcgc
     4141 agcctgctgg tgctgcaggt cggcagtcat gcggttgaag gcgtcggaca ggtctgcgat
     4201 ttccccgggc gcgcccacgt tatcgacgcg agccgaaaga tcaccaccgg cgacctgatc
     4261 cgcagctttg accagacgcc cgatgggggc agaaatggcg ctggctgccg acatgccgag
     4321 ccagacggcg ccgacgagga ccagaagcgc ggtctcgaga tagctgagcg caaaagcggt
     4381 ctggatacgg gcccggctgt cttccgcctc gcggtaccct tggatcgatt gatcagcggt
     4441 gcgcatctgc gacacgagac cgggcgccaa gggtcgaacg acatagagat aggcgtcgcc
     4501 gtagtccggc agcggataga gggcgcgaac ggtgtcgggg ttttcagtga cttcgaccgg
     4561 agcttccgag ccttcggccg cgatctccag cgccgagcga ggcggggcga catagggcgg
     4621 cgcatcagcc gtctcgcccc tcgccagtat ctcgccgtcg ccattcagga tgtagacggc
     4681 ggggtagccg aagaactcgg cgatctgggt cagggcgccg gtgaactgga tgcggttgtc
     4741 gaacacctga cgcacgccgc caagttcgtt ggacatggcg ccaagatcgc gcgtcatgcc
     4801 gccgccgacg tcgccgatat aggctttccc gatatcggcg ccgttttcga ccgcgtcctg
     4861 aacgttctgg ctgaaccact ggtccacgcc ccggttgacc agcacgccga ataccaacgc
     4921 gatcaggatg gccggcacga ctgccacgag tgagaacagc gcgacgaagc ggaggtggag
     4981 gcgagcgccg gggtcgctgc gctgggcgcg ggccagccgc atgacctggg cgccgacgat
     5041 gagcgcgagg ccgccgatca ggatcagatt ggcgagaaga atgtagagaa ccgcttggcc
     5101 agccactgcg cgggcgccgc cgtcggctcc ggcgccgggg gcgaccgcca ccagccagat
     5161 cgccgcagcg gccgtcacca ccgctatgct gtaggcagcg ccgaacagag cgcgcgctct
     5221 accatccgcg aaccgccgcc aaaggctggg ttccgcgtcg atgttcagcg cttcaagctg
     5281 ggcgcccggg gaagcgggtg tgtccgtcat gccgcatcag cttcaaccag ctgtggcgaa
     5341 aactcaacag gcgagttgct gaaatgcgcc agcggtagcc agacgtgttt caactctctg
     5401 attacaacct cggggtcgtc gatcctgcag aggcgggatt tagcctggcg acgctccagc
     5461 gggtcgacgg gccagggcgc ctgttcgatg taccagccga ggtgttttcg gaacatcttc
     5521 aggccgatag ggtggtcata gaaggcgacc gaggcgcgca ggtgttcgga gacgatggcc
     5581 agacgctcct cagggccggg ctcctgaagt tcggtcccgt cgatcagagc ctgttcgaga
     5641 tgagcagcca gccatgggcg gccatagaca ccgcgtccaa gcatcaagcc gtctgcgccg
     5701 gagcgtgaca gcgcttcgcg tgcctgatcc gccgtgatga tgtcgccgtt gactatgacg
     5761 gggatggata gcgccgcctt gacctcgcct atagcgtccc agtcggcgac gccggtgtaa
     5821 aactgctggc gggtgcggcc gtggacggtg acggccgcga cgccgaggtc ttccgcgcga
     5881 cgggcgatgt cgacggcgtt gcgatgggcg tcatcccagc cgaggcgcat cttgaccgtg
     5941 actgggcggc tgacggcccc cacggccgcc gcgatcaggc gcgacgcgag ttcgggcgtg
     6001 cgcatcaggg ccgagccaga cgcggagccg gtgacttcct tggcgggaca accaaaatta
     6061 agatcgatga tgtcggcgcc tgcggcttcg gccatgcgcg cgccctgtgc catcgcgtcc
     6121 ggatcgcgac cgacgagctg gatcaccgtc agcggcaggc ctgcgcccac cgctgcgcgc
     6181 cggactacgt caggtcgtcc gcgcgccagc tcggccgagg ccaccatctc ggtcgcgacg
     6241 taggccgcgc ccagcttcga ggcgagcagg cggaacggga ggtcggtgat gcccgtcatc
     6301 ggggccatca gcacccgtcc accaatggtc acgtcgccga tctgaaggct gggacgtgga
     6361 acaggatggg gacctaggtg tgctgggttt tgcaaactgt ccgccatcgg tccttttccg
     6421 ccgtttccag tccctctcgt caagcggaac cggggcgatt agaaggccgc catgacattc
     6481 gcctccatcg tcgtcgccgc aggaaccggt tcaagagccg ccaatgacgt ccgcatcgcc
     6541 aagcaatggc gtacgctggg cggcaaaccc gtgctgcgct ggtcggtcga ggccctgctg
     6601 aacgccgggg cggaagaggt cgtggtggcg gtttcggcgg acggccacga ggcggcggcg
     6661 gcggccttgg ctggtctgaa tggctggcgc tcggtcactg gtggtgagac gcgagccgaa
     6721 tcggtgcggg cggggctggc ggcgctgacc ggaccggacg atcagatcct gctggtccac
     6781 gatgccgcgc ggccactgct gagcgactcg gtgatccagc ggctgctgat ggcgttagag
     6841 acagcggacg gcgcactgcc cgccctgccc gtctcggata cgctgaagaa gacttcgggc
     6901 gacgccatca ccggcacggt cgatcgtgac ggcctgtggc gcgcgcagac gccgcaggcg
     6961 tttcgcaagg accggctgct ggccgcctgg gcgaactggc ccgtcagcga gacgccgacc
     7021 gacgactgcg ccgtggtcga gcgtgatggc ggcaaggtcg tggtcgttga gggtgaggcg
     7081 cgattgatga aactgacttt cccggaggat ttcgccatgg ccgaggccct gatcgctgct
     7141 ggaacacgga tgcagacccg cgtcggctcc ggcttcgacg cgcaccgctg gggtccgggc
     7201 ggctcggtgt ggctttgtgg ggtcgagatc ccgcatgacc agaccctgat cggccattcg
     7261 gacgccgacg ccgggttgca cgccctgacc gacgcgatcc tcggtgcgct tggcgacggc
     7321 gatatcggcg accatttccc gccgaccgat ccgaaatgga agggtgcgtc gtcggacctg
     7381 ttcctgtcct atgcggcgga caaggttgcc gcgcgtggcg gccgaatggt ccacgtcgac
     7441 gtcaccttga tctgcgagcg acccaaggtg aagccgcacc gtcaggccat gcgcgaacgc
     7501 gtcgccgccc tgctgtccct gccgctcgac gctgtcagcg tgaaggccac tacaaccgag
     7561 gccatgggct tcaccggccg tggcgaaggt ctggcggccc aggcgatcgc cacgattgag
     7621 ctgcctgcct agtaaggcgt ccggcgaatc tgccgccaca gggcgccgaa caggttcaca
     7681 tagtcgtctg tccatgggcg gacggtcgtg tcgggcagag tgctccagcg ggggtcggcg
     7741 cggaattcgg ccaagccttc cggtgtcggc gacagaataa tgacctcggt cgaggcctcg
     7801 gccatgtcgg gcgtattgcg gtcctcggcg tagagctgat gcaggtacgg ggcgttgagc
     7861 gccacagcgg ccgcctgcgt tggccggacg atgtcgaggt tacggttcga caagtgcagg
     7921 atgacgacgc ccgtcggctt gagcagcttc agatagccgg cgatggcttc ctcggtcagc
     7981 aggtgcgtgg gcacggcgtc ggacgagaag gcgtcgatca acagcagatc gtaggtcccc
     8041 gcaggctgtt tcgccatcgt cagccgtgcg tctccgagca cggtgtcgat cgctccgccg
     8101 gagccgtcgt tctgggcgca gccgttgatg aaggtgaacc attgcggatc gcgcgacagg
     8161 cggtcgacca taggatcgat ctcgaagaag gtcagccggt cgtcgcctcg cttgtagccg
     8221 gccatggcgc ccgacccctg ccccaccacg cccatacgca cgccgggcgc gcggcgctga
     8281 acgacctgtg tcgtctgacc gatcggggtg gagggtgcgt agtagagcgt cggcatacag
     8341 gcgaagcgcg ggtcgcgtgg ttgggcgccg tgcagcgtcg tgccgtgcat aagcacatgg
     8401 acatcacccc cgaggcggtc gtcgcgagtg ttggccaccc gcatgacgcc gaagaagctg
     8461 cgctccgtca aagcccagtc gtagcctcga ccgatggaca gggtggtcat ggcgatcagg
     8521 gcgcagatga tgacgaaggc cggcgtgcgg tcgcggatca ggaaggcggc gatcatcgcg
     8581 aaaagcagga tcagctgtgt cgtctgctcc atagaagcac tgatgttgcg gctccaggcc
     8641 caggcgagat cccagtcggc gtcgaacacg ctcttcaaaa ccagtggcga cgcgcagatc
     8701 aacaccgcca cgacgaaggc atagacctcc cacttgtgga tcggcgcttt gctcaggggg
     8761 cgagccagtc cgaccagcac cagcaccagc ggatattcca gaacggtggt gaagatcaca
     8821 ggagccagca gggcggtgaa ggctccacca accacgccac ccagcgacat cagcagatag
     8881 aactccg
//
LOCUS       DASVTM010000139         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_438892,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000139 DASVTM010000000
VERSION     DASVTM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438892"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..954)
                     /locus_tag="VF633_03920"
     CDS             complement(31..954)
                     /locus_tag="VF633_03920"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX8470238.1"
                     /translation="MTQSIKAAKTAVLYRMVMRQHTCPYGLKAKDLLKRSGFKVEDRH
                     LTTREATDAFKAEHGVATTPQTFINGERIGGYDDLRRHLGKSVADPKATSYWPVIVLF
                     PLTALMAMGASHAVLGSPFTLRAAEWFIGFSMVVLSLLKLQNVETFSTMFLNYDLLAK
                     RWVPYGYVYPFAEGLAGLLMVAGILTWLSVPIALVIGGIGAVSVFKAVYIDKRELKCA
                     CVGGSSNVPLGFVSLLENLMMVAMAVWMALGALGWAAGAHTGPSPAMAAHSVLVASET
                     ETPSTATYRAAHHAMMAGIAVPKKGRDTALA"
     gene            1010..1414
                     /locus_tag="VF633_03925"
     CDS             1010..1414
                     /locus_tag="VF633_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8470239.1"
                     /translation="MVQSQVPRPLTIGKLAEAGGVGVETVRFYQRKGLLSTPDRAEGF
                     RHYGDDDIRRLRFIRQAQTAGFTLGEIAELLELDAGEDRDRARRLAETRINALDARIA
                     DMEQARDALRRLARECGGGASGPCPILKSFEV"
     gene            complement(1803..1879)
                     /locus_tag="VF633_03930"
     tRNA            complement(1803..1879)
                     /locus_tag="VF633_03930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1843..1845),aa:Arg,seq:acg)
     gene            2047..3672
                     /locus_tag="VF633_03935"
     CDS             2047..3672
                     /locus_tag="VF633_03935"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HEX8470240.1"
                     /translation="MISVVSVAPFSDQKPGTSGLRKAVGVFQQPHYAETFIQSVFDVL
                     ADKTGATLVIGGDGRFLNREVIGKAIRMAAANGFGRVVIGRGGILSTPAASHLIRIRH
                     ALGGLILSASHNPGGPHGDFGIKYNIANGGPAPESVTAAIHARSLVIDRYMTLEGEVD
                     LEIEGESRLGEMVVEVVDPVADYADLMESLFDFDAIRNHRAEGFSLSFDAMSAVTGPY
                     AHEILERRLGFAPGSVVNGTPLEDFGGHHPDPNIVHARELYDRMMGPDAPDFGAASDG
                     DGDRNLVIGRGRFVAPSDALAILAANAHLAPGYARGLAGIARSMPTSAAADRVAAALG
                     IPLFETPTGWKFFGNLLDAGRVTLCGEESAGAGSDHVREKDGLWAVLLWLNILAVRRM
                     SVDDLVRDHWARFGRNYYVRHDYEEVSSEGADALMAGLRLNLGTLPGTEQQGLTIAEA
                     DDFAYTDPIDGSVSAHQGVRVMFEGGSRIVYRLSGTGTSGATLRVYIERYEPVGGDLD
                     AEPAVALSALVEASVALARIVEFTGRLTPDVAT"
BASE COUNT          675 a         1245 c         1269 g          689 t
ORIGIN      
        1 acaagcaccg cgaacggata tcggcttcgc tcatgccaag gccgtgtccc gccctttctt
       61 cgggacagcg atgccagcca tcatcgcgtg gtgggcggcg cggtacgtgg cggtcgatgg
      121 cgtttcggtc tccgacgcga cgagcacgga atgagcggcc atggcaggcg aaggccccgt
      181 gtgcgcgcct gccgcccagc cgagggcgcc cagcgccatc cagacggcca tggcgaccat
      241 catcaggttc tcaagcaggg agacgaagcc cagggggacg ttgctggagc cgccgacgca
      301 ggcgcacttg agttcgcgct tgtcgatgta gacggccttg aacacggaca ccgcgccgat
      361 ccctccgatg accagagcga ttggaaccga cagccaggtc agtatgccag ccaccatcag
      421 cagaccggcg aggccctcgg cgaagggata gacgtagccg taaggcaccc atcgcttggc
      481 cagcaggtcg tagttcagga acatagtcga gaaggtctcg acgttctgca gtttcagcag
      541 ggacagcacg accatggaga agccgatgaa ccactccgcc gcccgcaggg tgaaggggct
      601 tccgagcacg gcatggctgg cgcccatggc catgagggcc gtgaggggga acaggacgat
      661 tacgggccag tagctggtcg ccttcggatc agccacgctc ttgccgaggt ggcggcgcag
      721 gtcgtcgtag ccgccgatgc gttcgccgtt gatgaaggtc tggggcgtgg tcgctacccc
      781 gtgctccgcc ttgaaggcgt cagtcgcctc gcgcgtcgtc aggtggcggt cctcgacctt
      841 gaagcccgat cgcttcagca gatccttcgc tttcaggccg taagggcagg tgtgctgccg
      901 catcaccatg cggtacagaa ccgccgtctt cgccgcctta attgactgtg tcattgccga
      961 tctctcgcgt tccattggaa caggtcggct caaatatagg ttccgtacca tggtacagag
     1021 tcaagtcccc cgtccgctga ccatcggaaa gctggccgag gctggcggcg tgggcgtgga
     1081 gacggtgcgc ttctaccagc gcaaggggct gctttcgaca cccgaccggg ctgaaggctt
     1141 ccgccactat ggcgacgacg acatccggcg gctccggttc atccggcagg cccagacagc
     1201 cggcttcacc ctcggcgaaa tcgccgagct ccttgagttg gacgcgggcg aggatcgcga
     1261 ccgggcgcgc cggttggcgg agacgcggat aaatgccctc gacgctcgca ttgccgacat
     1321 ggagcaggct cgtgacgcac tcaggcgact ggccagggaa tgcggcggcg gtgcaagcgg
     1381 gccatgcccc atcctgaaat ctttcgaagt ctagccctgc gatcccgacg gcacgccggc
     1441 cttcaccccc gttgcctgat cccaccccgg tccacggcgc cctggtcccc gcggccaaat
     1501 gctgcaatcg tcttcgcgca tggcgaggtg ctcactaccg agaacctgct cgcgcgctga
     1561 cagccaccgt tcccgtccgt gctgagcgcg gggtggcaat ctcgctccgg tgttcaatga
     1621 tactgagagc gaccgaaccg ccgctaccaa gcagcaacgg atcgagtcag agagccagtc
     1681 aatccggctg gagaaacgtc tcggatttca acgttctagc cagtgggcca aggggcttta
     1741 caccccgcgg cgccacgcct cagtggcgcg gcatatatca gcttgttgac ttgtaagaca
     1801 aatggcgcgc ccggaacgat tcgaacgtcc gaccctcaga ttcgtagtct gatgctctat
     1861 ccagctgagc tacgggcgcg cacaggcctt tcggcgaggg cggctatcta gcggctggtt
     1921 cgggcgggcg caagcgactt cgtgagattt cgcccaagtc atcttcaggg atcgcgccgg
     1981 cgatgcaaat cgttggaagc tgtgctcaat agaggtggca ggctgtgcct ccaaaggaat
     2041 gaaaccttga tctcagttgt gtcagtagcc ccgttttccg accagaagcc gggcacatcg
     2101 ggtttgcgca aggcggtcgg agtgttccag cagccgcact acgccgaaac cttcatccag
     2161 tcggtcttcg acgtgctggc tgacaagacc ggcgcgacac tcgtcatcgg cggggatggc
     2221 cggtttctga accgtgaggt catcggcaag gcgatccgca tggcggcggc taacggcttc
     2281 ggtcgcgtgg tcatagggcg gggcggcatc ctgtcgacgc cggcggcgag ccacctcatc
     2341 cgaatccgac acgccctcgg gggcttgatc ctgtcggcca gccacaatcc cggcggcccg
     2401 catggcgatt tcggtatcaa gtacaatatc gccaatggcg gtccggcgcc tgaatcggtg
     2461 accgccgcga tccatgcgcg tagtctggtc atcgaccgct acatgacgct tgaaggtgag
     2521 gtcgatctcg agatcgaggg cgagagtcgt ctgggcgaga tggtggtcga ggtcgtcgat
     2581 ccggtcgccg attatgccga tctgatggag agcctgttcg atttcgacgc catccggaat
     2641 catcgcgccg aaggcttcag cctgtcgttc gacgccatga gcgcggtgac cggcccctat
     2701 gcgcacgaaa tcctcgaacg ccgcctcggc ttcgcgccgg ggtcggtggt caacggcacg
     2761 ccgctcgagg actttggcgg ccaccacccc gatcccaaca tcgtccatgc gcgcgagctc
     2821 tatgaccgca tgatgggccc ggacgcgccc gatttcggcg ccgcgtccga cggcgacggc
     2881 gaccgcaatc ttgtcatcgg tcgcggccgg ttcgtggccc cctccgacgc cctggcgatc
     2941 ctggccgcca acgcccacct ggcgcccggc tatgcccggg gtctggccgg tatcgcccgt
     3001 tccatgccga cgagcgcggc agccgaccgc gtcgcggcgg cgctcggcat cccccttttc
     3061 gagacgccga cgggctggaa attcttcggc aacctgttgg acgccgggcg ggtgacattg
     3121 tgcggcgagg agagcgcagg cgcaggctcg gatcacgtcc gcgaaaagga cggtctctgg
     3181 gccgtgctgc tgtggcttaa cattctggcc gtgcgccgga tgtccgtcga cgatctggtg
     3241 cgggaccact gggcgcggtt cggccgcaac tattacgtcc ggcacgacta cgaggaagtc
     3301 tcgtccgagg gcgcagacgc cctgatggcc gggctgcgtt tgaacctcgg gaccctgccc
     3361 ggcaccgagc aacaagggct gaccatcgcc gaggcggacg acttcgccta cacggatccc
     3421 atcgacggct cagtcagcgc gcatcagggc gtgagggtga tgttcgaggg cgggtcgcgc
     3481 atcgtctacc gcctgtccgg gacggggacc tcaggcgcga ccctgagggt ctatatcgaa
     3541 cgttacgaac ccgtgggcgg cgatctggac gcggagccgg cagtggcctt gtcggcgctg
     3601 gtcgaggcca gcgtcgccct cgcccggatc gtggagttca ccggccggct gacgcccgac
     3661 gtcgccacct gaaccgccta cggttcggct ccgtggctga cgattttatc ttccgctccg
     3721 gaagttgggt ccttcgaatg cctatctcga gtggaagtga aatcatgcca taaacccctt
     3781 gcggtctgcc aaggccgcag gttatagagc gcgccttccc gtccaaggaa agttgccttg
     3841 gacctacggc ggatgcgtag ctcagcggga gagcacct
//
LOCUS       DASVTM010000140        11795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_492726,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000140 DASVTM010000000
VERSION     DASVTM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492726"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /gene="modA"
                     /locus_tag="VF633_03940"
     CDS             <1..541
                     /gene="modA"
                     /locus_tag="VF633_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8470241.1"
                     /translation="APADLVVLAEEPWMDRLVTSGRVASSSRIDLLGNRLVLVAPSTA
                     RVDGNPLVWLKRTHGKLVIGDPGSVPAGAYAREWLQREQAWGGLQSSIVTAADVRAVR
                     GFVARGEAQLGMIYRSDAVGFKDIQIVFEPAAADQPEIVYPAALTTAAPPAAAAFLAW
                     LGTPEAGAVFRRYGFEVRF"
     gene            622..1317
                     /gene="modB"
                     /locus_tag="VF633_03945"
     CDS             622..1317
                     /gene="modB"
                     /locus_tag="VF633_03945"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX8470242.1"
                     /translation="MFAPLSPFEAEALLLSLRVAAASLAGVVPVALALALLLARGRFP
                     GKWLIEALVNLPLVLPPVVTGLALLILFGVRGPIGGWLYRTCGITLAFHWTGAALAAG
                     IMALPLVVRPVRLSLEAIDNRLEQAAAALGAPPLHVFLRITLPLAAPGLIAGALLGFA
                     RAFGEFGATVTFAGAIPGETQTLPVAIYTVLQRADGEHAALRLALLSVAVSMIALAVT
                     EWINRRALSRRGA"
     gene            1314..1949
                     /locus_tag="VF633_03950"
     CDS             1314..1949
                     /locus_tag="VF633_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8470243.1"
                     /translation="MSLALRVRVSRGDFDLDVAFDTDARALGVVGPSGAGKTTLLQGL
                     AGLIPTNDTRLVIGGKVVVDTLVGLNPPTHRRGVGYVFQDGRLFPHMTVGANVGFARS
                     YAVDPLTVAEALKLVDMEGFEHRRPAGLSGGEARRVAIARALLARPRLLLLDEPFSGL
                     DDARRETLIPYLVRLRDEVGIPMILVSHDERDVSALAQTILAIEHGRRVDP"
     gene            2102..3214
                     /locus_tag="VF633_03955"
     CDS             2102..3214
                     /locus_tag="VF633_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEX8470244.1"
                     /translation="MSVSVSRRLILTAAIATTLAGAVPDEALAATSYDAVVQRPGGRA
                     HPGVPSYPDLTAAIAAAPADGLQPFRILLTRGIWDGQVVVDKPFVHLIGEDRTGSVIN
                     HLAASGLAAPDGRNLGTFRTPTMFVRAPDFHARNLTINNAFDGLAEMSKSGGLHSHDG
                     AGPQAVALMLDKGSERASLIDCDILSYQDTLFPDAGNSLFERCLIVGSYDFIFGAGRA
                     WFDDCEIRSRPRPVDPVEGYIVAPSTPIDQPFGFVFHRCRLTKETGVLRGSVYLGRPW
                     RPSRVFADGRYGDPRFVAAATFLDCWMDDHIAPAGWTEMWYTGKDGNPRTMLQPEDVR
                     FSQYGSRGPGQAAFARGRALDQRSATLITRQAVIGF"
     gene            complement(3543..5588)
                     /locus_tag="VF633_03960"
     CDS             complement(3543..5588)
                     /locus_tag="VF633_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8470245.1"
                     /translation="MRIRDARLLVLTPLSLAFAAPALSQTAADAPAPEAEASQQGVLV
                     FTPDFFASQQPVTALDMVNRVPGFGVVDGDGSRGFQGSVGNILINGARSASKNDTGSS
                     VLSRTLVAQVERIELVRGGAPGIDMQGFSVVANVITRGQSSRQSIFTGNAVLYEGGND
                     TYGGTWQFTAMDGDRTWGFTLTDGFEVNDSNGVGPVVRRDAAGVLLRNEAFSNDQTSG
                     GNALRLNYASPFLGGQIDVTARYGVNDYETRSLQTSPTAQRESQYQEDGSEGEFGAVV
                     TRALGARLTSETRFIHEFSDTAGVSTNLTNLSGVGSPEQRFESDSNASETILRGLIRF
                     EQSTDLTWEFGGEVARNMLETDQALSVGSVTVPLPSASVKVQETRGEAFGKSTWRIRD
                     NLSVEGGLRLEGSTISQSGDADSEKTFSFIKPRLLATWTPGAANQFRIRFEREVGQLD
                     FNAFAASAELQNENVLGGNIDLEPEQRWISELAFERHFWGDGVVSITLRHDEISDAID
                     VIPLDEGLSATGNIGDGALDRLAVNIQAPLDRFGFRGGRISARNTWNHTEVTDPTSGK
                     ARPISTVPKSHAVMMVAQDIKTWSLQWSAAWVPLLGRTSYDPDVRFSWRGRDFLEGWV
                     EYKATRSLAIRAQINVWDDYQQTRTSYADRLTRPVGYTETRTIDPRTFYSLRLRKTF"
     gene            5690..7780
                     /locus_tag="VF633_03965"
     CDS             5690..7780
                     /locus_tag="VF633_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8470246.1"
                     /translation="MNDLSALLRRPIDLVSEPSFPLGSGVVHPASLEVIWPDTRCKLE
                     PRVMQVLVALNRRRGNPLSREELSRLCWDGRVVGDDALVRCIVKLRKVFQRDPVVEIG
                     SIPKVGYRLHLLSVVPELADTPLPLQSPRSVWRPLRWLLAGGGVAAAVALAVGAFVLM
                     KPPGALATEAMRPLTSEPGVETHPALSPDGRLLAYAGGAGFGTQRDIFLRGVDDGAPI
                     RLTNTEEDEMAPAWSPDARRIAFVRRIPGAPCEILVAPVPQGEPRVVGRCSVETMTRL
                     AWTRDGEILLSDRPTRDAVRRIRALSPDTGRMREVTSPPSTSLGDLDPIVTHRGDHMV
                     FRRAFSYGVADLYAAEVSGARLRPLTRDGWKAMGYAFTADDRTLVFSSNRGGDSGLWS
                     VDFKRPATPIRLSLGLLEFGRLSADKTGLMAVEAGDYRSNLFAWRAGAEPVALTNGNT
                     SDWDPDLSATGQLTFVSDQNGGSEIWSKRRDGQPSRLTDLKASSVFSPRWSPDGRSIA
                     FVASRSGRTDVYLMDAGGAGLRNVTSDGAVRDELAWSHNGDLLVPTLRKGGWRIERID
                     AQGQVSVVPNSAGVLTVKSAGPRGIYAVKKDDDRLWRLGAAGVVLSSPLIRIDRDAAW
                     TPTADGVYHLTGAPGGEADLRLTSWSGGGRDLQSLGRFGLKHSLAVSSTGDIVAARVV
                     RDEAALRAIQLGRS"
     gene            complement(7984..8202)
                     /locus_tag="VF633_03970"
     CDS             complement(7984..8202)
                     /locus_tag="VF633_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470247.1"
                     /translation="MRAIDVATELTGMRPRFILPIMFLGQKLGLEGNEIGIETIGRTI
                     GSITNAQEQQMIAGVERPRANYVGPLPR"
     gene            complement(8468..8728)
                     /locus_tag="VF633_03975"
     CDS             complement(8468..8728)
                     /locus_tag="VF633_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470248.1"
                     /translation="MTACSRCCRDEHANPLAYDLSPEGFARTAADALGWLAAILVDRD
                     RPTAEPLHSIAASRGASPAVSDVIRQALIQAADRELDRRLPG"
     gene            8975..9337
                     /locus_tag="VF633_03980"
     CDS             8975..9337
                     /locus_tag="VF633_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8470249.1"
                     /translation="MVELIPTHLDIVFHALGDGTRRQMLRNLVDGEQTVGELARPFDM
                     SLAAASKHVKSLEGAGLIRREIRGRTHYCRLDPGPLSRAFDWLGHYERLWTNNLDQLE
                     RLLRQDARPIPTSEGNGT"
     gene            9334..9864
                     /locus_tag="VF633_03985"
     CDS             9334..9864
                     /locus_tag="VF633_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8470250.1"
                     /translation="MTRSMTADAYGALTNPTTLTMQRLLPARIDRVWAYLTDGDLRAK
                     WFAAGDMPTSVGDAIELVWRNNDLTNPPGRIPEGMGAENRMLTRITELDPPHTLAIGW
                     GDGEVRFVLVEQGEDVLLTLTHTGIPDRARLLSFAPGWHMHFDILRARLTGTTPEPFW
                     DGIARLREDYIARLPA"
     gene            10002..10679
                     /locus_tag="VF633_03990"
     CDS             10002..10679
                     /locus_tag="VF633_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470251.1"
                     /translation="MPADLEIRYALSAAPPALREAATVYRLDPDQGYILARTGDNGLA
                     CLVQRTAWELLDFRDDIFIPLCYDAAGLEGHFKAIIDVARLRVEGLAPEEVKTRIEAS
                     YAGGGYALPGRTGLSYMVSPVMRTVGPPDLSVRTMAMPHLMFYAPGVTNADIAAAPNL
                     SDAASLMNPFVDRQGHDAQTYVIVMTGAAEKAQILEREAELVADLCAYRDVLCLRHSH
                     DHDRQRP"
     gene            complement(11221..>11795)
                     /locus_tag="VF633_03995"
     CDS             complement(11221..>11795)
                     /locus_tag="VF633_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8470252.1"
                     /translation="PSGVGKSTLIKVLIGLYPLNYGEILIDGRPLAFWNPRSFRRQIG
                     VVMQDDALLQGSLIENIALFDERPDMQRVEAAARTAAIYDEIVAMPMGFQSLVGDMGS
                     ALSGGQRQRLMIARALYRQPRILVLDEGTSQLDISNEQAINAALRNLNMTRIVVAHRP
                     ETLRAADRVLLLQAGQLVQVHPQPTPLKFR"
BASE COUNT         1947 a         3821 c         3807 g         2220 t
ORIGIN      
        1 ggcgccggca gatctcgtcg tgttggccga ggagccctgg atggacaggc tggtcacttc
       61 cgggcgcgtg gcgtcatcca gccgtatcga ccttctgggc aaccggttgg tgctggtggc
      121 cccgtcaact gcccgggtcg acggcaatcc tctggtctgg ctcaaacgca cgcacggaaa
      181 actggtcatc ggcgatcccg gcagcgttcc cgccggagcc tacgcccgcg aatggctgca
      241 gcgagagcaa gcctggggcg ggctgcaatc gtccattgtc accgccgccg acgtccgcgc
      301 cgtccgcgga tttgtagcgc gtggagaagc ccagctcggt atgatctacc gcagtgacgc
      361 ggttggcttc aaagacattc agatcgtatt tgagccggcc gccgccgatc agcccgagat
      421 tgtctacccc gcggccctga cgactgcagc gccgccagca gccgcggcat ttctggcttg
      481 gttggggacc cccgaagctg gcgccgtgtt caggcgctac ggcttcgagg tccggttttg
      541 atcggtggag ctgatctccc tcacgtggat ctatcgctgc cgatatccgc gccttaaagg
      601 ctggtttatg aggatacctt cctgttcgcg ccgctaagcc ccttcgaggc ggaggcgctg
      661 ttgctttcgc tgcgggtcgc ggctgcgtcg ctggcgggcg tcgttccggt cgcgctcgcc
      721 ctggccctcc tgctggctcg cgggaggttc ccgggcaaat ggctgatcga ggctttggtc
      781 aaccttcccc tcgtgctgcc gcccgttgtg acaggtctgg ccttgctcat ccttttcggc
      841 gtccgcgggc cgataggcgg atggctgtat cggacgtgcg ggatcactct ggctttccac
      901 tggacaggcg cagcgctggc ggctggcatc atggccctac cactggttgt gcgtccagtg
      961 cggctttctc tggaggcgat cgataatcga ctggagcaag cggccgctgc tctcggagcc
     1021 ccgccactgc atgtcttcct ccgcatcacc ctccccttgg cggcccccgg tctgatcgct
     1081 ggcgccttgc tcgggttcgc gcgagcattc ggagagttcg gcgcgacagt aacctttgcg
     1141 ggcgcgattc ctggcgagac ccagaccctg cccgtggcta tctacacggt gctgcaacgg
     1201 gctgacggcg agcacgccgc gctgcgcctg gccctgctgt cggtagcggt ctccatgatt
     1261 gctctggcgg tcaccgagtg gatcaaccga cgcgctctca gccggagggg cgcgtgagcc
     1321 ttgccctgcg tgttcgcgtc agccgtgggg acttcgatct cgacgtcgcc ttcgacaccg
     1381 acgcccgcgc gctcggcgtg gtcggcccct cgggcgcagg caagaccacc ctgttgcagg
     1441 gccttgcggg cctgatcccg acgaatgata cccgcctcgt catcggggga aaggtcgtgg
     1501 tggacaccct tgtcggactg aatcctccga cccaccgccg aggtgtcggc tatgtctttc
     1561 aggacggcag actgttcccg cacatgacgg tcggcgccaa cgtcggtttc gctcgatctt
     1621 acgccgtcga tccgctgacc gtcgccgagg ctctcaagct cgtggacatg gaggggttcg
     1681 aacatcgccg cccggcggga ctttcgggcg gcgaggcccg ccgcgtcgcc atcgcccggg
     1741 ctctgctggc ccgtccacgc ctgctgttgc tcgatgaacc cttctccggg ctggatgacg
     1801 cgaggcgtga gaccttgatc ccgtatctgg ttcgcttgcg cgatgaggtc gggattccga
     1861 tgatcctcgt gtcccacgac gagcgcgacg tttccgccct cgcgcagacg atactggcga
     1921 tcgagcatgg tcgccgggtt gacccctgac agaacccggg aaggcaaagg agcggtggtc
     1981 tactaccaca cgggccgtca gtataattcg gcgtccgcca cagcttctag aactcctcct
     2041 cccgaggatt ggacagcggc cggttcgccg ggcggcgccc gctttttcat ttgaggatcc
     2101 gatgtccgta tccgtctctc gccgtctgat cctcaccgct gcaatcgcca ccactctggc
     2161 cggggccgtg ccagatgaag cactggccgc gacatcttat gacgccgtcg ttcagcggcc
     2221 aggagggcga gcgcacccgg gcgtaccgtc ttatccggat ctcaccgccg ccatcgccgc
     2281 agcgcctgct gacgggctgc agccgttccg cattcttctc acgcgcggga tctgggacgg
     2341 tcaggtcgtg gtggacaagc cgttcgttca cctgatcggc gaggaccgga ccgggtcggt
     2401 tatcaatcac cttgcggcgt ccgggctggc cgcccccgac gggcgcaacc tgggcacgtt
     2461 ccgtaccccg acgatgttcg tccgcgcacc tgatttccac gcccgcaacc tgaccatcaa
     2521 caacgccttc gacggcctgg ccgagatgag caagtcgggc ggattgcatt cccacgacgg
     2581 cgcggggccg caggcggtag ccctgatgct cgacaagggt tccgaacgcg cctctctgat
     2641 cgactgcgat atcctgagct atcaggacac cttgtttccc gatgcgggga acagcctgtt
     2701 cgagcgctgc ctgatcgtcg gcagctacga cttcatattc ggcgcggggc gggcctggtt
     2761 cgatgactgt gaaatccgtt cacgcccccg cccggttgat cccgtcgaag gctatatcgt
     2821 tgcgcccagc acgcctatcg accagccctt cggctttgtc ttccaccgct gtcgactcac
     2881 taaggagacc ggggtcctga ggggctcggt ttatctgggg cggccctggc gaccatcccg
     2941 cgtcttcgcg gacgggcgct atggcgaccc ccgcttcgtc gccgctgcga ccttcctcga
     3001 ctgctggatg gacgaccata tcgccccggc cggctggacc gagatgtggt ataccggcaa
     3061 ggatggcaac ccgcgcacca tgctccagcc cgaagatgtg cggttctccc aatacgggtc
     3121 gcggggaccg gggcaggccg cgttcgcccg cgggagggcg cttgaccaga ggtcggcgac
     3181 actgattacg cgacaagcgg tgatcggatt ctgacgccgc cagagcgcaa ccgaaaacgc
     3241 catttgacat cctcacgcga tgtcgcaagt caggagatcc tgacgctgcg ctgggatttc
     3301 gtcgactggc gcaagggctg tcttcgcctt cccgaatcca aaactggcgc aaggtcgttc
     3361 ccctggcaga tcaggccatc gggatactca agcgacgttg ggacgagacc caacctccag
     3421 acgcctcacg aggccacaac tccggcgaag ccattctcca tcttggagtg aactcgcctt
     3481 gggttcttcc ggccctggtc ttctggactg aatagtgcgt ccattggcgg cctcaacagc
     3541 actcagaacg ttttacgaag acgcagcgag tagaaggttc gcggatcgat ggtgcgggtc
     3601 tcggtatagc cgaccggccg cgtcagacgg tcggcgtagg aggtgcgggt ctgctgataa
     3661 tcgtcccaga cgttgatctg ggcgcggatc gccaagctac gagtcgcctt gtactcgacc
     3721 catccctcca ggaagtcgcg gcccctccac gagaaccgga catccgggtc gtagctggtg
     3781 cgacccagca gcgggaccca ggcggcgctc cactgcagac tccacgtctt gatatcctgg
     3841 gcgaccatca tgaccgcgtg ggacttgggg acggtggaga ttgggcgcgc cttgccgctc
     3901 gtcgggtccg tcacctcggt gtggttccag gtgttgcgtg cgctgatccg gccgccacgg
     3961 aagccgaagc gatccaacgg cgcctgaatg ttcaccgcca gccgatccag tgcgccatcg
     4021 ccgatattac cggtcgccga caggccctca tccaacggga tgacgtcgat ggcgtcgctg
     4081 atctcgtcat ggcgcagggt gatgctgacg acgccgtcgc cccagaagtg gcgctcaaag
     4141 gccagttcgc tgatccagcg ctgttcgggt tccagatcga tgttgccgcc gaggacgttc
     4201 tcgttctgca gttcggccga ggcggcgaag gcgttgaagt ccagttggcc cacctcacgc
     4261 tcgaagcgga tgcggaactg gttggctgca ccgggcgtcc aggtcgccag caggcgcggc
     4321 ttgatgaagg agaaggtctt ctcgctgtcg gcgtcgcccg actggctgat ggtcgagcct
     4381 tccaggcgca agccgccttc gacggagaga ttgtcgcgga tccgccaggt gcttttgccg
     4441 aaagcctcgc cacgcgtctc ttgcaccttt actgaggccg acggcagggg aaccgtgacg
     4501 ctgccgacgc ttaaggcctg atcggtttcc aacatgttgc gggcgacctc gccgccgaac
     4561 tcccacgtca gatccgtcga ctgctcgaag cggatcagac cgcgcaagat ggtctcggag
     4621 gcgttgctgt cggactcaaa gcgctgctcc gggctgccga cgccagacag gttggtgaga
     4681 ttggtcgaga cgccggcggt gtcgctgaac tcgtggatga agcgcgtttc ggaggtcagg
     4741 cgggcgccga gggcgcgggt gacgaccgcc ccaaactcac cttcagagcc gtcttcctga
     4801 tactgagatt cacgctgcgc cgtgggcgag gtctgcaggc tccgggtttc atagtcattg
     4861 acgccgtagc gagcggtcac gtcgatctgc ccccctaaga agggactggc gtagttcagc
     4921 cgcagagcat taccgccgct tgtttggtcg ttggaaaagg cttcgttgcg cagcaggacg
     4981 cccgcggcgt cgcggcgaac tacggggccg acgccgttgg agtcgttgac ctcgaatcca
     5041 tcagtaaggg tgaagcccca ggtgcggtcg ccgtccatgg ccgtgaactg ccacgtgccg
     5101 ccgtaggtgt cgttgccgcc ctcgtacagg accgcgttgc cggtgaagat cgactggcgg
     5161 ctggactggc cgcgggtgat gacgttcgcg acgaccgaga aaccctgcat gtcgatgccg
     5221 ggggcgccgc cacgcaccag ctcaatccgc tcgacctggg cgaccagtgt gcgcgacagg
     5281 acgctggagc ctgtgtcgtt cttcgaggcc gagcgtgcgc cgttgatcag gatgttgccg
     5341 accgagcctt ggaaaccgcg gctgccgtcg ccatcaacca cgccgaagcc cggaacgcgg
     5401 ttgaccatgt ccagggccgt gaccggctgc tggctggcga agaagtcggg cgtaaagacc
     5461 agcacgccct gctggctggc ttcggcctcc ggcgccgggg cgtcggccgc agtctgcgag
     5521 agcgccggcg cagcgaacgc cagggacagt ggggtcagta caagcaggcg agcgtcgcgg
     5581 atgcgcatcg aaggtccgat caaggtggct ggtaatctcc gcggcgatag aagagattgg
     5641 cgttctggga gcgagggcgg ggggccgatc ttgaaccgat gattttccga tgaacgacct
     5701 gtcagctctg cttcgcaggc cgatcgacct ggtgtccgag ccgtcgtttc ccctcgggtc
     5761 gggcgtggtt catcccgcct cacttgaggt gatctggcct gacacgcgct gcaaacttga
     5821 accgcgcgtg atgcaggttc tggtcgcgct caaccgccgg cgcggcaatc cgctcagccg
     5881 tgaggaattg tcccgcctgt gctgggacgg acgggttgtg ggcgacgacg ccctggtgcg
     5941 atgcatcgtc aagctaagga aggttttcca gcgcgatccg gtcgtggaga tcggcagcat
     6001 cccaaaggtc ggctatcgcc tgcaccttct atccgtcgtg ccggagctgg ccgacacgcc
     6061 gctgccgttg cagtcacctc gatccgtttg gcggccgctg cggtggcttc tggcgggcgg
     6121 cggcgtcgcg gctgcggtcg ccttggccgt cggcgctttc gtcttgatga agccgccggg
     6181 cgcgctcgcg accgaggcca tgcgccccct gacctccgag cccggcgtcg agactcatcc
     6241 agctctttca ccggacggac gcctgctggc ctacgccggg ggcgccggtt ttgggaccca
     6301 gagagatatc ttcctgcgcg gcgtcgacga cggcgccccg atcagactga ccaacacgga
     6361 ggaggatgaa atggcgccgg cctggtcgcc cgacgcccgt cgcatcgcct ttgtgcggcg
     6421 tattcccggc gcgccttgcg agatcctcgt cgcgccagtg ccccaggggg agccgcgcgt
     6481 cgtcggtcgc tgctcggtgg aaaccatgac gcgtctggcc tggacccggg acggcgaaat
     6541 cctgctgtcc gaccggccaa cgcgcgacgc cgtgcgccgc atccgggccc tgtcccccga
     6601 cacgggacgg atgcgcgaag tcacctcgcc gccctccacc agcctgggag acctcgatcc
     6661 gatcgtcact caccggggcg atcacatggt tttcaggcgg gccttctcct atggggtcgc
     6721 tgatctttac gccgcagaag tgtccggggc ccgactgcga ccgcttaccc gcgatggttg
     6781 gaaggccatg ggatacgctt tcactgccga tgatcggacc cttgttttct ccagcaatcg
     6841 aggcggggat tccggcttgt ggtcggtgga cttcaaacgg ccggccacgc ccatccgcct
     6901 cagcctgggc ctgctcgaat tcggccgcct gtccgccgat aaaaccgggc tgatggcggt
     6961 cgaggccggc gactaccggt ccaacctgtt cgcctggcgc gccggtgctg aacccgtcgc
     7021 ccttacgaat ggcaatacca gcgactggga cccggacctt tccgcgacgg gccaactaac
     7081 cttcgtttcc gatcagaacg gcgggtcgga aatctggagc aagcgtcgcg acggccaacc
     7141 gtcgcgcctg accgacctga aggccagcag cgtattctcg ccccggtggt cgcccgacgg
     7201 ccggagcatc gcctttgtcg cgtcgcggag cggccgcacg gacgtctatc tgatggacgc
     7261 cggcggggcc ggccttcgca acgtcacgtc cgatggcgcg gtgcgcgatg aactggcctg
     7321 gagccataat ggcgacctcc tcgttcccac gctccgaaaa ggcggctggc ggatcgaacg
     7381 aatcgacgct caaggccagg tctcagtggt tccgaacagc gctggggtcc tgaccgtcaa
     7441 gagcgccgga cccagaggaa tctatgcggt gaaaaaggat gatgaccggc tctggcgcct
     7501 gggcgccgca ggcgtggttc tctcctcccc cctgatccgc atcgaccgcg atgcggcctg
     7561 gacgccgaca gcagacggcg tctaccacct gaccggagcc cccgggggcg aagccgacct
     7621 gcgcctgacc tcatggtccg gcgggggccg cgacctgcaa tctctcggta ggttcggcct
     7681 gaagcacagc ctggccgttt cctctacggg agacatcgtc gcggcgagag tggtccgcga
     7741 cgaggccgct ctgcgagcca ttcagttggg ccggagctga agctcaggcc cgttatcgga
     7801 cggcgcgagg tctgttttgc ggcgacgtcc cattgtcgga tcttggcgcc tctaagacat
     7861 cggcggcaac cgccactgat gggtcttggc ggctaagaac gtcggctctc ggtatgcatg
     7921 caaggctgcc gtgtggaccg atgccggagc gaagtgagga ctgcttggcg actgcgatga
     7981 acgctaacgg ggtaacggac cgacatagtt cgcccggggt cgttcgaccc ccgcgatcat
     8041 ctgctgctcc tgcgcgttag tgatcgagcc aatggtgcgg ccgatggtct ctatgccaat
     8101 ctcgttgccc tcaagtccaa gcttctgacc gaggaacata atcggcagga tgaacctggg
     8161 ccgcatcccg gtcagctcgg tcgcgacgtc gatcgcacgc atcaagcggt cgaggtccgg
     8221 gtcgcccgca aaggtgtgtt tgaacgcgtc gatcagcgcc tcggcccggg gggctccggt
     8281 gtcgtggatg tcgtatcgat caaacgccgg gattgtctca ccggcgcgaa gacgttgacg
     8341 acgacatcct ccggttttgg gctttcgacg atctccttca acagccgacg gatggtctcc
     8401 gtccgcttca cgcgaagctt ctgtccgcgt ccggcgatca tacccaccag aacgacggag
     8461 tagggcgtca ccccggtagc cgccgatcta actcacgatc cgccgcctgg atcagcgctt
     8521 gccgaatgac gtcactgacg gctggcgaag cgcctctgga ggcggcgatg gagtgtagcg
     8581 gctccgccgt aggtcggtcc cggtcgacga gtatggccgc cagccaaccc agggcatcgg
     8641 cggcggtgcg ggcgaaacct tccggagaca ggtcgtaggc caagggattg gcgtgctcgt
     8701 cgcggcagca tcgagagcac gcggtcattg gccggcatcc gggttaggac aacggccgaa
     8761 agcggacttt catgagttct gctttgggtg gggagcggac gttcgttctg accttggtta
     8821 tccgccacag attcagctag cgcgacgccc gcttcaacct ggctcgttcg gattaagagc
     8881 catgctctca acacgccggt catgaccctt cccagcagta aattaattca accgatcggt
     8941 tgactgttcc gcgtcctcca tctaattcaa ccaaatggtt gaacttatcc ccacccatct
     9001 tgacatcgta ttccacgcgc ttggcgacgg gacgcgccgt cagatgcttc gcaatctggt
     9061 cgatggagag cagacggtcg gcgaactcgc gcgaccgttc gacatgtcgc tcgcggccgc
     9121 ctccaaacac gtcaagtcgc tcgaaggtgc gggtctgata cgccgggaga tccgggggcg
     9181 tacgcattac tgtcggctcg accccggtcc attgtcgcgg gcgttcgact ggctcggcca
     9241 ctacgaacgc ctctggacca acaacctcga tcaactcgag cgcctgcttc gtcaggatgc
     9301 caggccaatc cctacttcag aaggaaacgg gacatgaccc ggtctatgac agccgacgcc
     9361 tatggcgctc tgaccaaccc caccaccttg acgatgcaac gccttctgcc tgccagaatt
     9421 gaccgggttt gggcctatct gacggatggc gacttgcggg ccaaatggtt cgctgcaggg
     9481 gatatgccga catctgtcgg ggacgccatt gagctggtct ggcgaaacaa cgacttgacc
     9541 aacccgccgg gccggatccc tgagggcatg ggggcggaga accggatgct gacgcgcatt
     9601 actgaactcg acccgccgca tacgcttgcc atcgggtggg gcgacggaga agtgcgcttc
     9661 gtccttgtcg agcaaggcga ggacgtcctg ctgaccctga cccacacggg catccccgat
     9721 cgcgcccgat tgctcagctt cgccccgggc tggcacatgc atttcgacat tctgcgagcg
     9781 cgtctcactg gaacgacgcc tgagcctttc tgggacggaa tagcccgtct tcgggaagac
     9841 tatatcgccc ggctgccggc gtgagggcgc cgtcgatgcg cagaccgatc ccaaggtgca
     9901 agagatcgcg acggcacgcg agtttcgtgc tcgctagcct gcttgtcctc gctggagcga
     9961 gcggagcggc cgcgcagagc gtgccgccga ggctcaccgc catgcccgcc gacctggaga
    10021 tccgctacgc tctcagcgca gcgccgccag cgcttcgcga ggcggccacg gtgtaccggc
    10081 tcgaccctga ccaaggctat atattggccc ggacgggcga caacggcctc gcctgcctgg
    10141 tgcagcgcac cgcctgggag ctcctcgact tccgcgacga tatcttcatc ccgctctgct
    10201 acgacgctgc cggcctggag gggcatttca aggcgatcat cgatgtcgct cgcctgaggg
    10261 tcgaaggcct cgcacccgag gaggtcaaga cgcgcatcga ggcctcatac gctggcggcg
    10321 gctacgcgct gcccggacgg acaggtctgt cctacatggt gtcgccggtg atgcggaccg
    10381 ttggtcctcc cgacctttcg gttcggacca tggcgatgcc tcatctcatg ttctacgctc
    10441 ccggcgtcac caacgcagac atcgcagccg cgccgaatct gtccgacgcc gccagcctga
    10501 tgaacccctt cgtcgaccga cagggtcatg acgcccagac ttatgtgatc gtcatgacgg
    10561 gggcggccga gaaagctcag attctcgagc gggaagccga attggtcgcc gatctctgcg
    10621 cttaccggga cgtcctctgc ttgcggcaca gccatgatca cgaccgacag cggccctgac
    10681 accgtccggt tttgctggcc acatcaatgc ctgtggctgg cgaacccccc tgaccacacg
    10741 tgtattgtcg cagcagggtg gcggcgaaca cgcagggccc ggccgccgag gtcgatgcgc
    10801 attacgcgcg atgggcattg agcgaacagg ttagcagttg ggaacatgcg cagtcagttt
    10861 tcattgcgcg acgcttctga agggcattac ttgatagact cggagacagc gggcgagtgg
    10921 atgtatttac gttcaaaatc atcgccgttg aaagattagt agaccctgca gctataaacg
    10981 cgtggcgaga ggcctgttcg gacgctagcc gatagctgtt tcgcaacgtc tgcaacgggt
    11041 cggtttgcgg cattggcttc tcggcgacac gtggaggtca gccggcgttc gcgagccatc
    11101 gagaggcaag ggcgaacgag aggtctcagg ctcccagcag gtcgtggcct ggcgccagag
    11161 ccgtcggttg ccatcgaccg gatgacgtca ggcgcgcgcc gataatgcta tccaggactg
    11221 tcatcggaat ttcaggggtg tcggctgcgg atgcacctgc accagttggc cggcctgcag
    11281 gagcaaaacg cggtctgccg cccgtaacgt ctcaggtcgg tgggcgacga cgatgcgcgt
    11341 catgttcaga tttctcagtg ctgcgttgat cgcctgctcg ttggaaatgt ccagctggga
    11401 tgtgccctcg tccaggacca gtatcctggg ctgacggtat agggcgcggg cgatcatgag
    11461 gcgctggcgc tgccctccgg acagcgcgga gcccatatcc ccgaccaggc tctgaaaccc
    11521 catcggcata gcgacgatct catcgtagat cgccgccgtc cgggcggccg cttcgacccg
    11581 ctgcatgtcg ggccgctcgt cgaacagggc gatgttctcg atcaaggatc cctgcagcag
    11641 ggcgtcgtcc tgcatcacga cgccgatctg acgccggaac gatcttggat tccagaatgc
    11701 aagcggccgc ccgtcgatca ggatctcgcc gtagttcagg ggataaaggc cgatcagaac
    11761 cttgatcaac gtcgatttcc ccacgccgct cggac
//
LOCUS       DASVTM010000141         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_172971,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000141 DASVTM010000000
VERSION     DASVTM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172971"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VF633_04000"
     CDS             complement(<1..882)
                     /locus_tag="VF633_04000"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX8470253.1"
                     /translation="MPASTAPLADDRLREEVRLLGGMLGEVIRDEGGQDLYDRIEAVR
                     QASVAYHRDPHSHPAARLERLLSELSLEQAVGLAHGFAVFSLLANVAEDRAGKRRART
                     QTAEGARADTLEGAMARLTAAGKTTEDARELLNDALISPVLTAHPSEVRRKSVLDRIA
                     AVSDLLDACDKDDLSCAPDVLTRGLRRQTVILWATRLVRTTGLVVQDEINTVVSFLDR
                     VFLRVAPEQLTEWRRALSAPDLNPFIRVGAWVGGDRDGNPNVDGTTLTAALATASRAV
                     LRYYLDEVNALGSELSLS"
     gene            1208..2773
                     /gene="ffh"
                     /locus_tag="VF633_04005"
     CDS             1208..2773
                     /gene="ffh"
                     /locus_tag="VF633_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX8470254.1"
                     /translation="MFEALNERLSGVFDRITGRGALSEKDVAEAMREVRVALLEADVA
                     LPVVKDFIAFATERATGEDVIRSVRPADQVVKIVYDGLIEMLGGEEPTPLNLNAAPPA
                     VILMAGLQGSGKTTTSAKLALRLTKFDRKKVMMASLDTRRPAAMEQLAQLGKQIDVAV
                     LPIVAGESAVQIAKRALTSAKLQGFDVLILDTAGRITLDEGLMNEVAEVAHVSNPVET
                     LLVADSLTGQDAVRTAKAFHDRLPLTGLVLTRADGDGRGGAMLSMRAVTGLPIKYMGS
                     GEKVDALDVFDARRVAGRILGQGDIVALVEKASQELDQSKAEAMARKLAKGQFDLNDL
                     AAQFQQMKKMGGLQGIMGMLPGVAKMKSQMADANVDDKSLARQEAIILSMTKAERKKP
                     DLLNASRRKRIARGAGVEVQEINRLLKQHRQMADMVKSMSKGGPRKMQQMAAMLGGLG
                     GGGGGGGMPGLPGGMAGLGGPDLNRLKAMGGGKTPEPSADDLKALQDRISGLGGGQLP
                     GGLPGLPGFPPKK"
     gene            2799..3383
                     /gene="rpsP"
                     /locus_tag="VF633_04010"
     CDS             2799..3383
                     /gene="rpsP"
                     /locus_tag="VF633_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX8470255.1"
                     /translation="MLKIRLARGGAKKRPYYYIVVADSHAPRDGKFLERVGTYNPMLA
                     KDGEKPRVTLKADRISEWLSKGAQPTDRVARFISQSADEALVGKVKWTQSNNPNKGAP
                     GKKAQERAAERSQREADRAEAEAAAKIEAAEAAVKAKEEAAAAAEAARNAPPVEEAPA
                     EEAAAEEAPVEAAAEEAPAAEEAPAADTEEKTEA"
     gene            3540..>4004
                     /gene="rimM"
                     /locus_tag="VF633_04015"
     CDS             3540..>4004
                     /gene="rimM"
                     /locus_tag="VF633_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506629.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX8470256.1"
                     /translation="MTNDKLILVGHVAGGFGVKGEIKITAYTADPLALTAYGPLLRAD
                     GSHALTVTSARPTKEGVVGRVKEVETKEQADALRNLKLYVARDRFPEPGEDEFYLADL
                     VGIEARDPEGIVLGTVRAVQNFGADDMLEISPAAGGQTWYLPFTKEATPELHL"
BASE COUNT          753 a         1368 c         1270 g          613 t
ORIGIN      
        1 cgacaggctc aactcggacc ccagcgcgtt cacctcgtcg agataatacc gcagcactgc
       61 acgcgacgcc gtcgccagcg ccgccgtcag ggtcgtgccg tcgacattgg gattgccgtc
      121 gcggtctccg cccacccagg cgccgacccg gatgaaggga ttgaggtccg gcgccgacag
      181 cgccctgcgc cattccgtca gttgctccgg cgcgacccgc aggaagacgc ggtcgaggaa
      241 cgagaccacg gtgttgatct cgtcctgcac caccagaccg gttgtccgca ccagccgcgt
      301 ggcccacagg atcaccgtct gccgccgaag cccgcgcgtc agcacatcgg gcgcgcagct
      361 caggtcgtcc ttgtcgcagg cgtccagcag atcggacacc gctgcgatcc ggtcgagcac
      421 gctcttgcgc cgaacctctg acgggtgagc ggtcagcacc ggcgagatca gggcatcgtt
      481 cagcaactcg cgcgcatctt cggtcgtctt gccagcggca gtcagccggg ccatcgcgcc
      541 ttccagcgtg tcggcccgcg ccccctctgc cgtctgcgtg cgtgcgcgtc gcttgccggc
      601 ccgatcctcg gcgacgttgg caagcaacga gaacaccgcg aacccgtgcg ccagccccac
      661 cgcctgctcc agcgacaact ccgacaacaa ccgctccagc cgggccgccg ggtgcgaatg
      721 cggatccctg tgataggcga ccgacgcctg cctgaccgcc tcgatccggt catagaggtc
      781 ctgtcccccc tcatcgcgaa tgacctcgcc cagcatcccg cccaacaggc gaacctcctc
      841 gcgcaagcga tcgtcagcta ggggcgcagt ggaagcaggc atggcgagtc tttcagtgaa
      901 ggcgggcggc agggaaggcc acaggatcga aacgggaccg tatcagggct tcgccgcctg
      961 accatcctgc accttaacga tgaaacggat tgtgcggttt ggtttcaaac gctcaattcc
     1021 cggcaagagt cgacaaacgg gcctctttcc tgcataagcc cgcgcttccg gcgcaattga
     1081 acctgcgtct ccacctgcac gtccccggct tcggtcggac gaggcaggcg agaaccctct
     1141 cccgacgtga tcgtcgccag aggccgcttt cgtatggagc acagttcgac cgcctaatga
     1201 ggtctatatg ttcgaggctc tgaacgagcg actgtcaggc gttttcgacc ggatcaccgg
     1261 tcgtggagcg ctgtccgaaa aggacgtggc cgaggcgatg cgcgaagtgc gcgtggcttt
     1321 gctggaggcc gacgtcgccc tgccggtggt caaggacttc atcgctttcg ccaccgaacg
     1381 ggccacgggc gaggacgtca tccgttcggt ccgtccggcc gatcaggtgg tcaagatcgt
     1441 ctatgacggc ctgatcgaga tgctgggcgg cgaagagccg accccactca acctgaacgc
     1501 cgccccgccc gccgtgattc tgatggccgg cctgcagggc tcgggcaaga caacgacctc
     1561 cgccaagctg gcgctgcgac tgacgaagtt tgatcgcaag aaggtcatga tggcctcgct
     1621 ggacacgcgt cgtccggccg cgatggaaca gctggcccag ctcggcaagc agatcgacgt
     1681 cgccgtgctg ccgatcgtgg cgggcgagag cgccgtccag atcgccaagc gcgcccttac
     1741 ttcagcgaag cttcagggtt tcgacgtcct gatcctcgac accgccggcc gcatcaccct
     1801 cgacgagggc ctgatgaacg aggtcgccga ggtcgcgcat gtgtcgaacc cggtcgagac
     1861 gctgctggtc gccgacagcc tgaccggtca ggacgccgtc cgcacggcca aggccttcca
     1921 cgaccgcctg cccctgaccg gcctcgttct gacccgcgcc gatggcgacg gtcgcggcgg
     1981 cgccatgctg tcgatgcgcg ccgtcaccgg cctgccgatc aaatacatgg gctcgggcga
     2041 gaaggtcgac gcgctcgacg tgttcgacgc ccgccgtgtc gccggtcgca ttctgggtca
     2101 gggcgatatc gtcgccctgg tcgaaaaggc ctcgcaggaa ctcgaccagt ccaaggccga
     2161 ggcgatggcc cgcaagctgg ccaagggcca gttcgacctc aacgacctcg ccgcccagtt
     2221 ccagcagatg aagaagatgg gcggtcttca gggcatcatg ggcatgctgc ccggtgtcgc
     2281 gaagatgaag tcccagatgg ccgacgccaa tgtcgacgac aagtcgctgg cgcgtcagga
     2341 agccatcatc ttgtcgatga ccaaggccga gcgcaagaag cccgacctgc tcaacgccag
     2401 ccgccggaag cgcatcgcca ggggcgccgg ggtcgaggtc caggagatca accgcctgct
     2461 taaacagcac cgccagatgg ccgacatggt caagtccatg tccaagggcg gtccgcgcaa
     2521 gatgcagcag atggccgcca tgctgggcgg tctcggcggc ggcggtggag gcggcggaat
     2581 gcccggcctt cccggcggca tggctggtct tggcggccct gacctcaacc gtttgaaggc
     2641 catgggaggc ggcaagacgc ccgaaccctc ggccgacgat ctgaaagctc ttcaggaccg
     2701 tatctcgggc ctgggcggcg gacaacttcc gggaggcctt ccgggtcttc cgggcttccc
     2761 acccaagaaa taatccgact taaagaaaga gactgaaaat gctgaagatt cgtctggccc
     2821 gcggtggcgc caagaagcgc ccctactact acatcgtcgt cgctgactcc cacgctcccc
     2881 gggacggcaa gttcctggaa cgcgtcggca cgtacaaccc gatgctcgcc aaggacggcg
     2941 aaaagccccg cgtgacgctg aaggccgacc gcatctcgga atggctgtcc aagggcgccc
     3001 aaccgaccga ccgcgtcgcg cgcttcatca gccagagcgc cgacgaagct ctggtcggca
     3061 aggtcaagtg gacccagtcg aacaacccga acaagggtgc tccgggcaag aaggctcagg
     3121 aacgcgccgc tgaacgctcg cagcgtgaag ccgaccgcgc cgaggccgaa gccgccgcca
     3181 agatcgaagc tgctgaagct gccgtgaagg ccaaggaaga agccgccgct gctgccgaag
     3241 ccgctcgcaa tgcgcctccg gtcgaagagg ctcctgctga agaagccgcc gccgaagaag
     3301 cacctgtcga agctgctgct gaagaggctc cggccgctga agaagcgcct gctgctgaca
     3361 ccgaagaaaa gaccgaagcc taaggcttca catccgctcc ggtcgaaagc ccgggggtca
     3421 ggcggcaggg ggcagtgttc gagatctcgg acactgcctt tctgttatcc ggcaccctat
     3481 tatcggagcg gaacttgatc tggacgccgg ccttggccgg aatgagcggg agcggtcgca
     3541 tgaccaacga caaactgatc ctcgtcggcc acgtcgccgg cggcttcggg gtcaagggcg
     3601 agatcaagat cacggcctat accgccgacc ctctggccct gaccgcctat ggcccgctgc
     3661 tgcgcgccga cggctcgcac gccctcaccg tgaccagcgc ccgcccaacc aaggaaggcg
     3721 tcgtcggacg ggtcaaggaa gtcgagacca aggagcaggc cgacgccctg cgcaatctga
     3781 aactctacgt cgcgcgcgac cgctttcccg agccgggcga ggacgagttc tacctcgccg
     3841 atctggtggg gatcgaggct cgtgaccccg aaggcatcgt tctcggcacg gtccgcgccg
     3901 tgcagaattt cggcgccgac gacatgctgg aaatctcgcc cgccgccggt ggccagacct
     3961 ggtacctgcc cttcaccaag gaggcgacgc cggaactgca tctc
//
LOCUS       DASVTM010000142        10100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_227409,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000142 DASVTM010000000
VERSION     DASVTM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227409"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VF633_04020"
     CDS             complement(<1..436)
                     /locus_tag="VF633_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1453 domain-containing protein"
                     /protein_id="HEX8470257.1"
                     /translation="MTPQQYIPLIAVGVALVLIVIRNRAPRTLRPQFLWVAPAIIIPL
                     MGLAIWGTGMSPGAAHAPFLALDWLVLTVGLLLGALAGWWRGKMTSIEKHADGTLKAQ
                     ASPVGIILIVGLLLGRRALNAFLEPHAATLGLNTTAVADAFLV"
     gene            complement(507..1514)
                     /locus_tag="VF633_04025"
     CDS             complement(507..1514)
                     /locus_tag="VF633_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8470258.1"
                     /translation="MKLASLKHGRDGRLVVVSNDLNWFTDAFLIAPTLQAALDDWERC
                     EPLLQALAESLEHEAVPRGRFREHEAAAPLPRAYQWADGSAYVNHVELVRKARGAEMP
                     ASFWTDPLMYQGGSDGFLAPRDPIPLADPAWGCDLEAEIVVVTGDVPQGVNAADALAY
                     IRLVGLVNDVSLRNLIPAELGKGFGFVQSKPASALSPVFVTTDTLGDRWKDGKLSGEL
                     NVQLNGQDFGKADAGTDMTFDFGTLIAHLAKTRPLVAGTIIGSGTVSNKDADGGPGKP
                     VSQGGLGYSCIAEVRTVETLQKGAPVTPFLTHGDTVRIEMLDDKHRSIFGAIEQTVEA
                     A"
     gene            complement(1574..2308)
                     /locus_tag="VF633_04030"
     CDS             complement(1574..2308)
                     /locus_tag="VF633_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470259.1"
                     /translation="MSAPTPTEFVSLEGAYTPEGSGLFFSLSPMMGATMVVMLGLALL
                     LGWFWGRRQRPTDDPSEAIYGTIRKALTEATAAPRDTVIASARKAQSTIKAQLGHVLG
                     LAHGLAGPFGALGPALDGIDPSHREPEPPQHDAPAKSSIERVVIKARDVFISSPALSS
                     AHEHDADHGHGDTEGHGAKPHGGGHREGQGGAHWSGHGPGKPLDTATQIDRVRAAIHD
                     LSDHWSDKAARVGELRAARKQLTRAS"
     gene            complement(2414..2680)
                     /locus_tag="VF633_04035"
     CDS             complement(2414..2680)
                     /locus_tag="VF633_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470260.1"
                     /translation="MSFADDELAAMAEEELASACALTWPELKRITPWGDSFEGFAPSG
                     RTVEVERRYLWALDPKGAIVVEVEVRDAAARTGVETTAILEPPV"
     gene            complement(2677..3876)
                     /locus_tag="VF633_04040"
     CDS             complement(2677..3876)
                     /locus_tag="VF633_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8470261.1"
                     /translation="MRLFEKLVGPALGLSLMAGPAFAQTADAPRWSFAIHGGAGVIER
                     TNLSPEQDAAYRSALRKALEAGGKVLDGGGSAMGAVQAAIEIMEDDPLFNAGRGAVFT
                     AAGRNELDAAVMNGADLAAGAVAGLTTTRHPIAAARAVMEQSPHVMLIGQGADSFAAS
                     AGLEQVEPSFFFTQRRWDQLVKLLTDQHLPIPLPPAGAPVSPAVGMAENDASAPPLNE
                     RKFGTVGAVALDNQGHLAAGTSTGGTTGKRWGRVGDVPVIGAGTYASNRDGCAVSATG
                     DGEYFIRATVARDICARISAMAGTHDANVRTAVEQADAANTGDTDSVAFVDELYPQAV
                     AAQAEIDDADSLGGKGGVIVMATDGVPAFAMSTSGMYRGEISADNPTPRVAIYADEQP
                     RPLELSQ"
     gene            complement(3895..4035)
                     /locus_tag="VF633_04045"
     CDS             complement(3895..4035)
                     /locus_tag="VF633_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470262.1"
                     /translation="MAGVLAGEYWLGGGFWVVLATGMVCGFAPIVIYRLWKWWHHRTP
                     RP"
     gene            complement(4091..5206)
                     /gene="hppD"
                     /locus_tag="VF633_04050"
     CDS             complement(4091..5206)
                     /gene="hppD"
                     /locus_tag="VF633_04050"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX8470263.1"
                     /translation="MTPAATQDLTTPDATIAAADNPLGVDGFEFVEFTGPDPQAMIRQ
                     LETMGFVQTHVNPRTNVVRLKQGDISFLVHRTPTGQAGEFARDHGPSANGMAFRVNDA
                     KAAYDSAVARGGHPAEAHAGGALGEGAYVLRGIGGSLLYLIDAYGETGSLYDTWDEIE
                     GWEEEERKSNVGLHLLDHLTHNVKRGQMRTWSGFYGDVFAFEEQKYFDIKGKATGLFS
                     QAMIAPDRAIRIPLNESQDDNSQIEEFLKRYNGEGIQHIALATDDIFATVEAMRARGV
                     EFQDTIETYFELIDKRLPNHGHDVERMRANRILIDGSDEEGLLLQIFTTDVFGPIFFE
                     IIQRKGNEGFGNGNFQALFDSIELDQIRRGVIKVDAN"
     gene            5400..5981
                     /locus_tag="VF633_04055"
     CDS             5400..5981
                     /locus_tag="VF633_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX8470264.1"
                     /translation="MLRKLYDWVFEQARKPYAEKTMAVVSFAEASFFPIPPDVMLAPM
                     ILSRPDRAYRYAAVCSIASLLGALFGYAIGMFLEPVAVKLMAFFGHGMGIEVYQSWFA
                     TNGFLVILGKGLTPIPFKLVTIAAGVAHFNLGLFIAAVAITRTARFFLEAWILKTWGP
                     RMLEIVEKRLALFFVLAVVLLVGGFLAIKLLGH"
     gene            6039..6602
                     /locus_tag="VF633_04060"
     CDS             6039..6602
                     /locus_tag="VF633_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX8470265.1"
                     /translation="MIEIYRLLTRWWTAFALVISLAMLGAAHASERLWGLTPCNLCLK
                     QREVLWGAVTIAAVATLWAIVSRASRGTPRIASFLLFAVFLTGTITAVFHTGGEMKWW
                     HLPATCSGAGDMASLESLTALALGTGPTIKVAMCDAVTWRFAGLSMSSWNAIVSACLA
                     GFSLLAAKRPKDARAPRNGAEVMVKAA"
     gene            6607..7194
                     /locus_tag="VF633_04065"
     CDS             6607..7194
                     /locus_tag="VF633_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX8470266.1"
                     /translation="MTDTSNQARTPRPPLPRPGRGSTRARMAEILRVDHAGEYAAVQI
                     YRAQRAVFEAAKGKEAVAADMAHMQDQEAVHLARFDALLNDRRVRPTLMVPAWRLAAQ
                     ALGAGTALMGEKAAHACTEAVESVIEEHYADQIAELKDRDPDLAAELERFRAEEMEHH
                     DHAVAHGSREAPAYRLLSAVIKAGCKVAIKVSERV"
     gene            complement(7204..8220)
                     /locus_tag="VF633_04070"
     CDS             complement(7204..8220)
                     /locus_tag="VF633_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX8470267.1"
                     /translation="MTSAVPRSAKRLLLAWGLMTLAACSGSMGVPLPYMPAVGGWIPQ
                     DVATKSVSLGLPGGAVLATGVTFAGGIEIVADAGSPLHSLSDMKLTGDGGFISVSDVG
                     DLVRAELRLDATGRPVGIDGPRARRLTLQDGTPIADKQMGDAEGLTLLPDGELLVSFE
                     RDHRIWNYGPLAALRNPIPVRKPDFSFPENDGMEGMAVAPHGWRVNGESGGVWDCSIA
                     VCTVVTAPPTPLISDSDFRITGMDRDPATAPGQGWFVVQRAYAPPINARARIRRMAAD
                     GTLGPVLVELKLPGTTDNFEGIAAETRNGPNGEVTRLYILSDDNFNAVQRTLMLAFDV
                     TPAR"
     gene            complement(8217..>10100)
                     /gene="cobT"
                     /locus_tag="VF633_04075"
     CDS             complement(8217..>10100)
                     /gene="cobT"
                     /locus_tag="VF633_04075"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX8470268.1"
                     /translation="APETPAEIFKRALAHAARALAEQSELEVVFGSDGPKLSNGLLTL
                     PHPPRDPSSADSATIRGQADRLALRLANHDEVLNARLRPLDSRAAEVFEAVEQARIEA
                     VGSNSLAGVRANMNAALLTRLERTGALRAEADRVPVAEAVALLVRERLTGQRSPDGAG
                     AMLDLVRQELETKAGAQLDRLAEVAENQGAFGKLLKDVLRALELDPGDGRGDDDSEDP
                     GEDKPDPQEPDSGDDQDEEEDEGGGEGGQSMDGSSEDQTAEEERDSTSDGSPAEMDGD
                     TTDDGPELSEGDLPNRQQTADDGRVIDAYRVFTTQFDETIDAADLCDPMELERLRSLL
                     DQQLTALSSIVARLANKLQRRLMAQQNRTWTFDLEEGMLDTARLTRIITDPTAPLSFK
                     AESESPFRDTVVTLLLDNSGSMRGRPIMVAAVCADILARTLERCGVKVEILGFTTRAW
                     KGGQAREAWISAGKPAMPGRLNDLRHIIYKSADAPWRRAKKNLGLMMREGLLKENIDG
                     EALTWAHDRLKSRPEARQILMVISDGSPVDDSTQSANAALYLDKHLRQVIEQIETRSP
                     VELIAIGIGHDVTRWYRRAVTIVDVEQLGGVMIEKLAELFDTEPKTISRKGGGRTASK
                     RAA"
BASE COUNT         1619 a         3241 c         3412 g         1828 t
ORIGIN      
        1 acaccaggaa ggcatcggca accgcggtgg tgttcaggcc aagcgtggcg gcgtgcggct
       61 caaggaaggc gttcaaggcg cggcggccca gcagcaggcc gacgatcagg atgatgccga
      121 caggcgaggc ctgggctttc agcgtgccgt ctgcatgttt ctcgatgctc gtcatcttgc
      181 cacgccacca gcccgctagt gcgccaagga ggaggccgac ggtgagcacg agccagtcaa
      241 gcgcgaggaa gggtgcgtga gccgcgccgg ggctcatgcc tgtgccccag atagccagcc
      301 ccatgagcgg gatgatgatg gcgggtgcta cccacagaaa ctgaggccgc aaggtcctcg
      361 gcgcgcggtt cctgatcacg atcagcacga gagccacgcc cacggcgatg agcggaatat
      421 attgctgcgg cgtcatagtt attgtccccc tgcggcgaga cgccgcaggc cagattacgt
      481 cgagagaacc gcctgtggga agagcctcag gcggcttcga ccgtctgttc gatggcgccg
      541 aaaatcgacc ggtgtttgtc gtccagcatc tcgatgcgga cggtatcgcc atgagtcagg
      601 aacggggtca ctggcgcgcc cttttgcagc gtttcgacgg tgcgcacctc ggcgatgcag
      661 ctatagccaa ggccgccttg ggataccggc ttgccggggc caccgtcggc gtccttgttg
      721 gacacggtgc ctgagccgat gatcgtgccg gcgaccagcg ggcgcgtttt ggccagatgg
      781 gcgatcaggg ttccgaagtc gaaggtcatg tcggtcccgg cgtcagcctt gccgaagtcc
      841 tgaccgttca gttgcacgtt tagttcgccc gacagcttgc cgtctttcca gcggtcgccg
      901 agtgtgtccg tcgtgacgaa aactggcgac agggcgctgg cgggcttgga ctggacgaag
      961 ccgaagccct tgcccagttc ggcgggtatc aggttacgca gcgagacgtc gttgaccagc
     1021 ccgacgaggc ggatgtaggc gagcgcatcg gcggcgttga cgccctgcgg cacatcaccg
     1081 gtgaccacga cgatctcggc ctcgaggtca cagccccagg cgggatcggc caaggggatc
     1141 gggtcgcgcg gggcgagaaa cccgtcggaa ccaccctgat acatcagcgg atcggtccag
     1201 aaactggcgg gcatttcggc cccgcgcgcc ttacgcacca gttcgacatg gttcacatag
     1261 gccgagccgt ccgcccactg atatgcacgg ggcagggggg cggcggcctc gtgctcccgg
     1321 aagcgaccgc gcggcacggc ctcgtgttca aggctctcgg ccaaggcctg aagcagcggt
     1381 tcgcaccgct cccagtcatc gagcgccgcc tgcagtgtcg gggcgatcag gaaggcgtcc
     1441 gtaaaccagt tcaggtcgtt ggagacgacg acgagacgac cgtcacggcc gtgcttgagc
     1501 gaggcgagct tcatgcctct ggcttaagcc gatttttccg gaggggaaac cgtagtcgaa
     1561 acctgatcaa tgatcaggag gcgcgcgtca gctgtttgcg cgctgcgcgg agttcgccaa
     1621 cccttgcggc cttatcgctc cagtgatcgg acagatcgtg gatggccgcc cggacccggt
     1681 cgatctgggt ggcggtgtcg agtggcttgc cgggaccgtg accgctccaa tgggcgcccc
     1741 cctggccctc tcggtgaccg ccgccatgtg gcttggctcc gtgcccttcg gtatcgccgt
     1801 ggccgtgatc tgcatcgtgc tcgtgtgctg aggacagagc ggggctgctg atgaaaacat
     1861 cgcgcgcttt gatgacgacc ctttcgatgc tcgatttggc gggcgcatcg tgttgtggcg
     1921 gttcgggctc gcgatggctg gggtcgatgc cgtcgagcgc gggaccgagt gcgccaaagg
     1981 gtccggccag cccgtgagca aggcccagta catggccaag ctgagccttg attgtcgact
     2041 gagccttgcg cgcagatgca atgacggtat cgcggggcgc ggcggtcgct tcggtgaggg
     2101 ctttcctgat cgtaccgtag atggcctccg atggatcgtc ggtcggtcgt tggcgacgtc
     2161 cccaaaacca gcccaacaac agggccagac ctagcatgac caccattgtg gcgcccatca
     2221 ttggcgacag gctgaagaac aggccgctac cctcgggcgt ataagctcct tcgaggctga
     2281 cgaactcggt cggggtcggg gctgacatgc ggcgatcctc tccgaacaac gatctcggag
     2341 cgccatcatg gcctaacggc tacgcttcgc aacgaaaagg ttaacgctgt tgtgcgaatg
     2401 ccgtggcatc gcttcagacg ggcggctcca ggatcgccgt ggtctcgacg ccggtgcggg
     2461 cggcagcgtc gcgcacctcg acctcgacca cgatggctcc tttcgggtct agcgcccaca
     2521 ggtagcgccg ctcgacttcg accgtccgcc cagatggtgc gaagccctca aagctgtcgc
     2581 cccaaggcgt gatccgcttc agctccggcc aggtaagggc gcaggcggac gccagctcct
     2641 cctcggccat ggcggccagt tcgtcgtcgg caaaactcat tgggaaagct caagcgggcg
     2701 tggctgttcg tccgcataga tcgcgacgcg cggagttgga ttatcggcgg atatttcgcc
     2761 gcgatacatg ccggaggtgg acatcgcaaa ggccgggaca ccatccgtgg ccatgactat
     2821 gacgccgccc ttgccgccga ggctgtcagc gtcatcgatc tcggcctggg cggcgacagc
     2881 ctgcggatac aattcgtcca cgaacgcaac ggagtcggta tcacccgtgt tggcggcgtc
     2941 agcctgttcc acggctgtgc gaacgttagc gtcatgtgtt ccggccatgg cagaaattcg
     3001 cgcgcagata tcccgcgcca cggtcgcccg gatgaaatac tcgccgtcgc cggtggccga
     3061 gaccgcgcag ccatcgcggt tggaggcgta tgtgcccgcg ccgatcaccg gcacgtctcc
     3121 aacccggccc cagcgcttgc cggttgtccc gccggtcgag gtgccggccg ccagatgacc
     3181 ctggttgtcc agagctaccg cgccgacggt gccgaacttg cgctcgttca gcggaggggc
     3241 tgaggcgtcg ttttcagcca tccctacagc gggcgagacg ggcgccccgg ccggtggcaa
     3301 ggggatgggc agatgctggt cggtcagcag cttgaccaac tggtcccaac gcctctgggt
     3361 gaagaagaac gaaggctcca cctgctccag tccggccgag gcggcgaagc tgtcggcgcc
     3421 ctgaccgatc agcatcacgt gcggcgattg ctccatcacc gcccgggcgg cggcgatcgg
     3481 atggcgggta gtggtcagac cggcgacggc cccggcagcc aggtcagcgc cgttcatgac
     3541 agcagcgtcc agctcattgc gcccggcggc ggtgaagacc gcgcctcgtc cggcgttgaa
     3601 cagaggatcg tcctccatga tctcgattgc cgcctgaacc gcgcccatcg ccgagccgcc
     3661 gccatccaga accttgccgc ccgcctccaa cgcctttctc aaggcggagc ggtaggccgc
     3721 gtcctgttcc ggtgagagat tggtccgctc gatcacgccc gcgccgccgt ggatggcgaa
     3781 tgaccagcga ggcgcatcgg ccgtctgggc gaatgccggg ccagccatca gtgagagacc
     3841 gagagccggt ccgaccagtt tttcaaacag gcgcattatg agctccgatc cggctcaggg
     3901 ccgtggggtc cggtgatgcc accacttcca caggcggtag atcacgatcg gcgcaaaacc
     3961 gcagaccatg ccggtggcca gaaccaccca gaacccgccg cccagccagt attctccggc
     4021 cagcaccccg gccacggccg cgaggaccgg gccgaggtac ggcagccacg agggaggctt
     4081 gcgcggcagc ctaattggcg tcgaccttga ttacgccccg gcggatctgg tccagctcga
     4141 tcgagtcgaa cagggcctgg aaattgccgt tgccgaagcc ttcgttgccc ttgcgctgga
     4201 tgatctcgaa gaagatcggg ccgaagacgt cggtggtgaa gatctgcagc agcaggcctt
     4261 cctcgtccga accgtcgatc agaatgcggt tggcgcgcat ccgctctacg tcatgcccgt
     4321 ggttgggcag gcgtttgtcg atcagttcga aataggtctc gatggtgtcc tggaactcga
     4381 cgccacgcgc gcgcatcgcc tcgactgtgg cgaagatgtc gtcggtggcc agagcgatgt
     4441 gctggatgcc ctcgccgttg tagcgtttca ggaactcctc gatctgggag ttgtcgtcct
     4501 ggctctcgtt cagggggatg cggatcgcgc gatcgggcgc gatcatggcc tgcgagaaca
     4561 ggccggtcgc cttgcccttg atgtcgaaat acttttgctc ctcgaaggcg aagacgtcgc
     4621 cgtagaagcc ggaccatgtg cgcatctggc cgcgtttgac gttgtgggtc aggtgatcga
     4681 gcaggtgcag gccgacgttc gacttgcgct cttcttcttc ccagccctcg atctcgtccc
     4741 aggtgtcata gagtgacccg gtctcgccat aggcgtcgat cagatacaac agcgagccgc
     4801 cgataccgcg cagcacatag gcgccttcgc ccagcgcgcc gccggcgtga gcttcagccg
     4861 gatggccgcc gcgcgcgacg gcgctgtcgt aagccgcctt ggcgtcattg acgcggaaag
     4921 ccatgccgtt ggccgaaggg ccgtggtcgc gcgcgaactc gcccgcctga ccggtcgggg
     4981 tgcggtggac gaggaagctg atgtcgccct gcttcagccg cacgacgttg gtgcgcggat
     5041 taacgtgggt ctggacgaag cccatggtct ccagctggcg gatcatggcc tgcggatcag
     5101 ggcctgtgaa ctcgacgaat tcgaaaccgt cgacgcccaa tgggttgtcg gcggcggcga
     5161 tggtggcatc gggcgtggtc aggtcctggg tggcggcggg cgtcatgcgg cgtctctcca
     5221 cgaatatgtt catgcggcgg tcgccgctgt tggccggaac ctagtgtcgg aagccggctt
     5281 cgccaaggcg ttggatattt cattgcgtct gcgaaggccg tttaggcggt gcggcgccgg
     5341 agcggatgga ccctgggtcc ggtcgttgct agggctgacg tcgctttccg gggatcttca
     5401 tgctgcgcaa actctacgac tgggtcttcg aacaggcgcg caagccctat gccgagaaga
     5461 cgatggcggt cgtgtccttc gccgaggctt cgttcttccc catcccgccc gacgtgatgc
     5521 tggcgccgat gatcctgtcg cgaccggacc gggcctatcg gtatgcggcg gtgtgttcga
     5581 tcgcttctct gctcggcgcc ttgttcggtt acgccatcgg catgtttctg gagcccgtag
     5641 ccgtcaagct gatggcgttc ttcgggcatg gtatgggcat cgaagtctat cagtcgtggt
     5701 tcgccaccaa cggcttcctt gtcatcctcg gcaaggggct gacccctatc ccgttcaagc
     5761 tggtgaccat cgcggccggg gttgcgcatt tcaatctcgg cctgttcatc gcggcggtgg
     5821 ccatcacccg cacagcccgc ttcttcctcg aggcgtggat cctcaagacg tggggaccaa
     5881 ggatgctgga gatcgtcgaa aaacggctgg cgctgttctt cgttctggcc gtcgtgctgc
     5941 tggtcggcgg tttcctggcg atcaagctgc tcgggcattg aggaacgtcg caatcgtcct
     6001 gttggagcat tgatcgggtc ggagtaggac tatttccgat gatcgagatt tatcgcctcc
     6061 tgacccgctg gtggacagca ttcgctctgg tgatttctct cgccatgctg ggtgcggccc
     6121 atgcgtccga gcgtctatgg ggcctcaccc cgtgcaacct ctgcctcaaa cagcgtgagg
     6181 tcctatgggg cgcggtgacc atcgcggcag tcgcgaccct gtgggccatc gtcagccgcg
     6241 ccagccgggg tacaccgcgt atcgcatcct tcctgctgtt cgccgtcttc ctgaccggga
     6301 cgatcaccgc agtcttccac accggtggcg agatgaaatg gtggcacctg ccggcgacct
     6361 gttcgggtgc gggcgacatg gccagtctcg aaagcctgac ggcgttggca cttggcaccg
     6421 gtccgacgat caaggtggcg atgtgcgacg ccgtgacatg gcgcttcgca ggcctgtcga
     6481 tgtcatcgtg gaacgccatc gtctcggcct gtctggcggg cttcagcctg cttgcagcca
     6541 aacgccccaa ggacgcccgc gccccgcgca acggcgccga ggttatggtc aaggcggcct
     6601 gaaacgatga cagacacctc caatcaagcc cgcacccctc gtccgccgct gccgcgtccc
     6661 ggacggggct cgacccgggc gcggatggcc gaaatcctgc gtgtcgatca cgccggagaa
     6721 tatgcggcgg ttcagatcta tcgcgcccag cgcgccgtgt ttgaagcggc caagggcaaa
     6781 gaagccgtcg ccgccgacat ggcccatatg caggatcagg aagcggtgca tctggcccgt
     6841 ttcgacgccc tgctgaacga ccgccgcgtg cgtccgacgc tgatggtccc ggcctggcgg
     6901 ctggcggctc aggcgcttgg ggccggtacg gccctgatgg gcgagaaggc tgcgcacgcc
     6961 tgcaccgaag ccgtcgagag cgtgatcgag gagcattacg ccgatcagat cgccgagctg
     7021 aaggatcgtg atcccgacct cgccgccgag ttagagcggt tccgcgccga ggagatggaa
     7081 catcacgacc acgccgtcgc ccatggcagc cgcgaagcgc ccgcctatcg ccttttgagc
     7141 gctgtgatca aggcggggtg caaggtggcg atcaaggtca gcgaacgcgt ctgacgcctc
     7201 cgactagcgg gcaggcgtga cgtcaaaagc cagcatcagc gtacgctgca ccgcgttgaa
     7261 gttgtcgtcg gacaggatgt agaggcgcgt cacctcgccg ttcggaccgt tgcgggtctc
     7321 ggcggcgatg ccttcgaaat tgtcggtcgt gccgggcagc ttcaactcga ccagcaccgg
     7381 cccgagcgtc ccgtcggcgg ccatgcggcg gatgcgcgct ctagcgttga tgggcggagc
     7441 ataggcgcgc tgaaccacga accagccctg tcccggagcc gtcgccggat cgcggtccat
     7501 gccggtgatg cggaaatcgc tgtcggaaat gaggggtgtc gggggcgcgg tgacgacggt
     7561 gcagacggcg atcgaacaat cccacacgcc gccgctctca ccattaacgc gccatccgtg
     7621 cggggcgacg gccatgcctt ccatcccgtc gttctcggga aaggagaagt cgggtttgcg
     7681 cactggtatc gggttgcgaa gggcggcaag cgggccgtag ttccagatgc ggtggtcgcg
     7741 ttcgaaactg accagcaact ccccgtcggg caacagggtg aggccttcgg cgtcccccat
     7801 ctgcttgtcc gctatcggtg tcccgtcctg cagtgtcagg cggcgggcgc ggggtccgtc
     7861 gatgccgacc ggcctgcccg tcgcgtcgag ccgcagttcg gcccggacga ggtcgccgac
     7921 atccgacacg ctgatgaagc cgccgtcgcc ggtcagtttc atgtccgaca ggctgtgcag
     7981 cggtgatcca gcgtccgcca cgatctcgat gccgcctgca aaggtgacgc cggtcgccag
     8041 cactgcgccg ccgggcaggc cgagactgac cgatttcgtc gcgacgtcct gaggaatcca
     8101 gccgccgacc gcaggcatat agggcagggg cacgcccatc gaaccggaac aggctgcgag
     8161 ggtcatcaga ccccaggcga gcagcagcct cttcgccgac ctcgggacgg ccgaggtcat
     8221 gcggcgcgct tcgaggcggt acggccgccg cccttgcgcg agatcgtctt gggctcggtg
     8281 tcgaacagct cggccagttt ctcgatcatg acgccgccga gctgttcgac atcgacgatg
     8341 gttacggcgc ggcggtacca gcgggtgacg tcgtggccga tgccgatggc gatcagttcg
     8401 acaggcgaac gggtctcgat ctgctcgatg acctgacgca ggtgcttgtc cagatacagc
     8461 gccgcattgg ccgactgggt cgagtcgtcc accggtgaac cgtcggagat caccatcagg
     8521 atctggcggg cttcggggcg tgatttcagc cggtcgtgcg cccaggtcaa ggcctcgccg
     8581 tcgatgtttt ccttcagcag accctcgcgc atcatcaggc cgaggttctt cttggcccgc
     8641 cgccacggcg catccgccga cttgtagatg atgtggcgca ggtcgttcag gcgacccggc
     8701 atggcgggtt tgccggcgct gatccacgcc tcgcgcgcct gtccgccctt ccacgcccgg
     8761 gtggtgaagc cgaggatctc aaccttgacg ccgcagcgtt ccagcgtcct cgccagaatg
     8821 tcggcgcaga ccgccgcgac catgatcggc cgcccgcgca tcgagccgga gttgtccagc
     8881 aacagggtca cgaccgtgtc gcggaacggg ctctcgctct cggccttgaa gctgagcggc
     8941 gcggtcgggt cggtgatgat ccgggtcagg cgcgccgtgt ccagcatccc ttcctccaga
     9001 tcgaaggtcc aggtgcggtt ctgctgcgcc atcagccgcc gctgcagctt gttggccaac
     9061 cgcgcgacga tgctggagag ggccgtcagc tgctgatcca gcaggctgcg caggcgttcc
     9121 agctccatcg ggtcgcacag gtcggcggcg tcgatggtct cgtcgaactg ggtcgtgaag
     9181 acgcggtagg catcaatcac ccgaccatcg tcggcggtct gttgccggtt cggcaaatcg
     9241 ccttcggaca gctcggggcc gtcgtcggtt gtgtcgccgt ccatctcagc gggcgagccg
     9301 tcggaggtgc tgtcgcgttc ttcctcggcg gtctggtcct cggacgagcc gtccatcgac
     9361 tggccgccct cgccgccgcc ttcgtcttct tcctcgtcct gatcgtcgcc ggaatcgggt
     9421 tcctgaggat cgggcttgtc ctcgcccggg tcctcggagt cgtcgtcgcc gcgcccgtcg
     9481 ccgggatcga gttcgagcgc ccgcaggaca tccttgagca gcttgccgaa cgcgccctgg
     9541 ttctctgcga cttcggccag acgatcgagc tgggcgcccg ccttggtctc cagttcctga
     9601 cgcacgaggt cgagcatggc gccggcgccg tcggggctgc gctggccggt cagccgctcc
     9661 cgcaccagca gggccaccgc ctcggcgacc ggcacccggt cggcctcggc gcgaagagcg
     9721 ccggtccgtt ccagccgcgt caacagggcg gcgttcatat tggcccgcac gcccgcgagc
     9781 gagttggagc cgacggcctc gatacgggcc tgttcgacgg cctcgaacac ctcggcggct
     9841 cggctgtcga gagggcgtag gcgggcgttc aggacttcgt cgtgattggc cagccgcagg
     9901 gccagccggt cggcctgacc gcggatcgtg gcgctgtcgg cgctggaggg atcgcggggc
     9961 ggatgcggca gcgtcagcag accgttggag agcttgggcc catccgagcc gaacacgacc
    10021 tccagctccg actgctcggc cagcgcccgg gccgcgtgag ccagagcgcg cttgaagatt
    10081 tcggcgggag tttcgggagc
//
LOCUS       DASVTM010000143         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_68879, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000143 DASVTM010000000
VERSION     DASVTM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68879"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VF633_04080"
     CDS             complement(<1..292)
                     /locus_tag="VF633_04080"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HEX8470269.1"
                     /translation="MTTASIASGLYVGTVFHRRLRPRVHALKYKIYMLLIDLDEAASL
                     IQRLKWLVSGKFGLMSLNLSDFGDRSNMPLRQQIEGSLAAAGIPGGGPIRLLT"
     gene            complement(289..1683)
                     /locus_tag="VF633_04085"
     CDS             complement(289..1683)
                     /locus_tag="VF633_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8470270.1"
                     /translation="MYDRLSPVEQTPKSRRHIAVVGSGVSGLSAAWLLSQAHDVTLYE
                     PGERLGGHAHTIDVPTPDGIVPVDTGFIVFNEPNYPNFTALLNYLDVPSKPAHMSFAV
                     SMNDGGFEYSSHGAKGLFAQKRNLASPKFWRMLLDLKRFHRTAEADLCDLDVSLCSLG
                     DYLNKGRYGAAFRDKHLLPQAAAIWSSSSCKMADYPAAAFIRFYRNHRLLEVDLKPNW
                     KTVDAGSRAYIDALAREFRGRIVSDAVARVERLPGQVTIHTASGKSETFDGVVLATHS
                     DQALALLDQPTADETRLLGAICYSKNRVVMHRDPKLMPKRRAVWAAWNYVGAGKKGGG
                     KAAEASEDHPSVTYWMNLLQGLPGAPVFVTLNPEAEPAPATIIVDQEFDHPIFNSAAL
                     TAQQELWSLQGVQNTWFAGAWFGSGFHEDGLQVGLAVAEAIGGVKRPWSVENESGRIP
                     LKIAVAQAPQAASA"
     gene            1834..3051
                     /locus_tag="VF633_04090"
     CDS             1834..3051
                     /locus_tag="VF633_04090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8470271.1"
                     /translation="MTAPSLSDAVGVRAASAPRPAELLLWVLQKVGQDWRGRGLTIQT
                     PDRQEYRFGPSSPEPGRLIVHDWRAMRRGLASGDIGLAEGYMAGEWDTPDLAALLAVF
                     ADNYERLDRFANGNAIGRAINWVVHRMGKLNTRAGSKKNIVAHYDLGNAFYGRWLDPG
                     MTYSSALWTRPEQSLEAAQREKYAALATSIDLKPEHTVLEIGCGWGGFAEYAAGEIGC
                     QVVGLTISPSQHQFAAERMMRAGLSDKVEIRLQDYRDVTETFDRVVSIEMFEAVGEKW
                     WPTYFDVVRSRLKPDGKAGLQIITIRDDLFAGYRERVDFIQKYVFPGGMLPSEAVLKD
                     VIETCGLGIAATTRFGQDYARTLHLWAKQFREVDSGMNTVFDRQWLFYLAYCEAGFST
                     RRTNVVQMTLCAR"
BASE COUNT          784 a         1293 c         1276 g          735 t
ORIGIN      
        1 tggtcagcaa ccggatcggc ccgccgccgg gaatgccggc ggctgccaga ctgccttcga
       61 tctgttgacg caacggcata tttgagcggt cgccgaagtc ggacaggttg agcgacatca
      121 ggccgaactt gccgctcacc agccatttga gtcgctgtat caggctcgcc gcctcgtcga
      181 ggtcgatcag gagcatgtag atcttgtatt tcagcgcatg gacacgcggc cgcagccggc
      241 gatggaagac cgtaccgaca tagagacccg atgcgatcga cgccgttgtc atgcggaggc
      301 cgcctgcgga gcttgagcca ccgcgatctt cagcgggatc cgaccgctct cgttttcgac
      361 cgaccacggc ctcttcacgc cgccgatcgc ctcggcgacc gccagaccga cctgcaagcc
      421 gtcctcatgg aaccctgatc cgaaccaagc cccggcgaac caggtgttct gaacgccctg
      481 tagcgaccac agctcttgtt gcgccgtcag cgccgcgctg ttgaagatcg ggtgatcgaa
      541 ctcctgatcg acgatgatgg tcgcgggggc gggctcggct tccgggttca gggtcacgaa
      601 caccggggcg ccgggcagac cctgcagcag gttcatccag taggtgacgc tgggatggtc
      661 ctcgctggct tccgcggcct tgccgccccc cttcttcccc gccccaacgt agttccacgc
      721 cgcccagacc gcgcgacgct tcggcatcag cttcggatca cgatgcatga ccacccgatt
      781 cttgctgtag caaatcgccc ccagcagccg cgtctcgtcc gccgtcggtt gatccagcaa
      841 ggccaaggcc tgatccgaat gagtcgccag gacgacgcca tcgaaggtct cggacttccc
      901 cgaggcggtg tgaatggtca cctgacccgg aagacgctcg acccgggcca ctgcgtcgct
      961 gacgatccgt ccgcgaaact cgcgcgccag ggcatcgata taggcgcggc tgccggcatc
     1021 aaccgtcttc cagttcggct tcaggtccac ctcaagcagg cggtggttac gatagaaccg
     1081 gatgaaggcc gccgccggat agtccgccat cttgcacgat gacgacgacc agatcgcagc
     1141 cgcttgaggc aacagatgct tgtcgcggaa ggcggcgccg tagcgtccct tgttcagata
     1201 atcgccgagc gaacacaacg acacgtcgag atcgcagaga tcggcctcgg ccgtccggtg
     1261 aaaccgtttc agatccagca gcatgcgcca gaatttgggg ctcgccagat tgcgtttctg
     1321 ggcgaacagc cccttggcgc cgtggcttga atactcgaac ccgccgtcgt tcatcgacac
     1381 tgcaaacgac atgtgcgcgg gcttggatgg cacatccaga tagttaagca gcgcggtgaa
     1441 gttggggtag ttgggttcgt tgaagacgat gaagccggtg tcgacaggca cgatgccatc
     1501 cggcgtcggc acgtcgatcg tatgggcatg accgcccagc cgttcgcccg gttcgtagag
     1561 ggtgacatcg tgggcctgtg acaacagcca cgccgcagaa agccccgaca ctcccgaccc
     1621 cacgacggcg atatggcgtc gtgacttcgg ggtttgttca acgggcgata gtcgatcgta
     1681 catgatggct cgcaagcagg ccgacgttca gccggacgag gtcagctacg aaacggcgcc
     1741 gcttccggat gcctgagaaa atcgcgcatc catttcgaag cctgaccgta gcttcgcctg
     1801 tccgaccgga tgcgtgactg cgcggagatc aagatgaccg ccccttcgtt gagtgatgcc
     1861 gttggcgttc gggcggcaag tgcgccgcga ccggcggagc tattgctatg ggtgcttcag
     1921 aaggtggggc aggactggcg cggtcgcggc ctgaccatcc agacaccgga tcgtcaggag
     1981 taccggttcg gcccgtcgtc ccccgagccg ggccggctga tcgtacatga ctggcgtgcc
     2041 atgcgtcggg gtctggcgag cggcgatatc ggcctggccg agggctatat ggccggtgaa
     2101 tgggatacgc ccgatctggc cgccctgctg gcggtattcg ccgacaacta cgaacgcctc
     2161 gaccggttcg ccaatggcaa cgccatcggc cgcgcgatca actgggtcgt acatcggatg
     2221 ggcaagctga acacacgcgc cgggtcgaag aagaacatcg tggcccacta cgatctgggc
     2281 aacgccttct acgggcgctg gctagaccct ggcatgacct attcgtcggc cctgtggacc
     2341 aggccggaac agtcgctcga agcggcccag cgtgagaaat acgcagcact cgccaccagc
     2401 atcgacttga agcctgagca cacagtgctg gaaatcggct gcggctgggg cggattcgcc
     2461 gaatacgcag cgggcgagat cgggtgccag gtcgtcgggc tgacaatctc cccatcccag
     2521 catcagttcg cggccgagcg gatgatgaga gccgggctct ccgacaaggt cgagatccgc
     2581 ctgcaggatt atcgcgacgt gacggagacc ttcgaccggg tcgtctcgat cgagatgttc
     2641 gaggccgtcg gcgagaagtg gtggccgacg tatttcgacg tggtccgcag ccgactgaag
     2701 ccggacggca aggccgggct gcagatcatc accatccgcg acgacctgtt cgccggctat
     2761 cgcgagcgtg tggacttcat ccagaaatac gtcttccccg gcggcatgct gcccagcgag
     2821 gcggtgctga aggacgtcat agagacgtgc ggtctcggca tcgccgccac gacccggttc
     2881 ggtcaggatt acgcccgcac cctgcacctt tgggccaaac agtttcgtga ggtcgacagc
     2941 ggcatgaaca ccgtcttcga ccgccagtgg ctgttctacc tggcctattg cgaggccggt
     3001 ttctccacgc gtcgaacgaa cgtcgtgcag atgacgctgt gcgcgcgata gcggtggagc
     3061 ggcgccgatg acgcggtcac tgcctgtatc catcctccgg tgacttggaa cgccaggcaa
     3121 aagcggtcct ggacagcacc tctgccaacc gggcgatcct cggcggcttt aagggcgtgt
     3181 gcggcagctg cggtgtagag gcctctggtc ctgcgtcttc agccagggat ttaccctgca
     3241 tggcttgcgc gcagatcgca cagaacaggg gcatggttac ggatcgcctc atcggcccgg
     3301 ccacgcgaac tcgactcgcg cggcgctgat ctgcccggct gaacttccgc ctgaagcggg
     3361 aacagaccag gcctgccctc ccgctgatga tagactgagc ctgggacggc aagacggttg
     3421 gacgcatggt catgatcgca gggaagggca gccggaagtc cggcgcggcg tcgatgatgg
     3481 cttacccttc ctgcgggcgg cggactcagt cgcagccttt gtcattcagg ggcccccggc
     3541 ccggcgcgta taggtgatcc gcccggcgcg gatctgatcg cccatctgaa gcgccacgac
     3601 gttgtagtcg agctgaagat cgaacgtcga cgcggccaac aagcagacct tccgaagcgc
     3661 ggatacgagt caagagcgga ggtcaacatc gagggccgag cagcattctg cgggcccccc
     3721 taaaggcaac gttcgcactc cagaccgatc cagtcgaagg cctggccgca gtttgcttac
     3781 tttcgcgagc aagaatgacg gctgagcgat ctcaatcttc tcgtatcgac aaccttttgg
     3841 ctcgcctcag acacccgcct cgagatgaaa cagcctgaaa tcatctcttg ccacgtcagg
     3901 caggctcaat cggtttcaaa cgccttcttt gtcgccggtc ggggacctgc agcgtctggc
     3961 gccccccttc tgcccccctt ggcgaagagt ggcaaaatat catccgtaca cagatactga
     4021 tgaaaacata aacgtttgtt ttaaaagcaa aaaatggcgc acccgacagg attcgaacct
     4081 gtgacccc
//
LOCUS       DASVTM010000144         6729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_599851,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000144 DASVTM010000000
VERSION     DASVTM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599851"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VF633_04095"
     CDS             <1..985
                     /locus_tag="VF633_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX8470272.1"
                     /translation="REQYIADVAAKTKLGRPLKVVCACGNGTAGVFAPQALRLMGCEV
                     IEMDTDLDYTFPKYNPNPEDHVMLMQMAAKVRETGADLALGFDGDGDRCGVVDDTGEE
                     IFADKIGLMLARDLSAIYKNVTFVVDVKSTGLYKTDEILKANGATTVYWKTGHSYIKR
                     KTAELGALAGFEKSGHFFLSGPLGHGYDDGIVAAGAVLAMLDRNAGKTLSELKTALPD
                     AWTSLTMSPHCSDETKYQVLEKLVAEYEDLFAAGGSVLGRKIVEVITVNGARVHLEDG
                     SWVLIRASSNKPEMVVVVESMRSADDMRDLFRQEMKPRLAAHGVVEYNQEI"
     gene            complement(1030..2490)
                     /locus_tag="VF633_04100"
     CDS             complement(1030..2490)
                     /locus_tag="VF633_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8470273.1"
                     /translation="MSLGDDLAGKLRLSPEWRERLTPHAPDGLSSRLLLLTVAFTLAV
                     EALIIAPSAASFHERWLTDRLQAAELASVGVEALPYNAVDDTTAESMLRIGGVSAVAI
                     SDQGVMRQLLQAPNLPRAPDFIDLRGRGLTARLWDPVVTLMGDPDRSIRVRAKPRYRS
                     GDFIDVVAPAEPLRMELRAFLLNSLLVSILISIVAGGLLYAGLAFLVLQPLRRVTRSI
                     EGFAANVETVAPTPSDRHDEIGRVERELARMQDEVRQSLRARSRLVALGEAVAKINHD
                     LRNMLTSAQMASERLASSADPQVAMAMPRLERALSRAAALTRNVLDYGKSEEPAPVLT
                     RIPLATAASNAAEDAGLDPAGVRLSKSIPARFAINADSDQLHRILVNLMRNARQAIEA
                     DATRGDRKGKGVVRIEALTDGEVVVIRIADDGPGIPQRLSERLFEPFVSGKASDGTGL
                     GLTISRELAANHGGELRLVDTGPGGTTFELTLPNAI"
     gene            complement(2528..3250)
                     /locus_tag="VF633_04105"
     CDS             complement(2528..3250)
                     /locus_tag="VF633_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX8470274.1"
                     /translation="MNRLRRFAPLIILACLMALIFGMGWNRYLSLETLRLHGGELRAF
                     TADHWIVSLLVLMAIFTLATASVVPGVFFVTITAGYLFGPWVGGVSTSIAATLGALIV
                     YGVARSALGKSMRERAEQNAGLMQKVCLAIDKETFWYVLASRLAVVVPFHMINIAAGV
                     MGVRLLPYTVATVIGLLPAHIIYCWIGARLNTLLATDPNPDFQALFGQFWAPMMGVFV
                     LAIVLPFALKAAQAWLVPKVAR"
     gene            3396..3530
                     /gene="rpmH"
                     /locus_tag="VF633_04110"
     CDS             3396..3530
                     /gene="rpmH"
                     /locus_tag="VF633_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX8470275.1"
                     /translation="MKRTYQPSRLVRKRRHGFRSRMATKNGQKIVARRRAKGRKKLTA
                     "
     gene            3565..3984
                     /locus_tag="VF633_04115"
     CDS             3565..3984
                     /locus_tag="VF633_04115"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX8470276.1"
                     /translation="MTDVLKIERLTSRPQFLAAAKGVSLARGAVVVQRLGRADGDPVV
                     RVGFTATRKVGGAVVRNRAKRRLREAARALVPSHGVPGSDYVLIARMGTPGRDWLRLL
                     DDVKSALTRLATSPAPSVGNSGDKPNAAARDTTTQDR"
     gene            3989..5815
                     /gene="yidC"
                     /locus_tag="VF633_04120"
     CDS             3989..5815
                     /gene="yidC"
                     /locus_tag="VF633_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX8470277.1"
                     /translation="MKLDNARNTVIFIVCTVVILILYQVFVLQPQANSRKAALAAESS
                     QPVRPDASAAIGPQTTPTSGNFVTDRTQALGGAARVPISTPTLRGSLSLQGGRIDDLF
                     LTKYRTTIKPDAGLEELFRPQGMEHAFFAQFGWTGPNIPGGVPGPNTVWSLTNGSTLT
                     PATPVTLTWDNGQGLRFTRIVSIDDQYMFAVSDTVANLGTQVVTIAPYGRVERQGVPP
                     ALGKTQILHEGAIGTFSTADGKGYSTEQLKYKAWEKKPLEAHTSKGGWLGITDKYWMA
                     ALIPPQTEIVTAQFKVDDAQRFDIHTAQMLGASHTIQPGQTITEQQHLFAGAKRNEIL
                     AGYEKSLGLPRFIYAIDWGFLFFLTRPIFLLVEFFYGLVGNFGVAILLLTLTIKLIMF
                     PLANKSYESLSKMRKLQPKMEAIKAAHKDDPAAQQRETMALYQSEKINPLAGCLPIVV
                     QIPVFYALYKVLYVTIEMRHAPFFGWIRDLSDRDPTNIWNLFGLIPWDPATAPLIGNF
                     LSPHVGGGFTLALSVLAIIYGLTMWLQQAMNPPAPDPIQRQIFMFMPLIFTFIMAAFP
                     AGLLVYWAWNNILSIVQQYFIMHRFKAENPIDTFFSRLKKAT"
     gene            5817..6455
                     /gene="yihA"
                     /locus_tag="VF633_04125"
     CDS             5817..6455
                     /gene="yihA"
                     /locus_tag="VF633_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX8470278.1"
                     /translation="MEYSDEEIEAARVLFERQATFVMGAAKIEQLPEPNLPEICFAGR
                     SNVGKSSLINGLVSMHKLARASNEPGRTREVNFFDLDGKLRLVDLPGYGWAKASKTTV
                     KKFQDLGRDYLRGRVTLKRVYLLIDARHGLKAVDGEALEALDLAAVSYQIVLTKADKI
                     KPAEGLAMQEKTLKAISKRPAAFPAVALTSAEKGFGMPELRAEIMRTTGAEI"
     gene            6534..>6729
                     /locus_tag="VF633_04130"
     CDS             6534..>6729
                     /locus_tag="VF633_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470279.1"
                     /translation="MTDPAHPEGPGVWTLLQDHVTHVMWQQDETDGAGAMASRFWVIN
                     PPEQLMFDTFDEAEAMFEQLS"
BASE COUNT         1239 a         2261 c         2107 g         1122 t
ORIGIN      
        1 tcgcgagcaa tatatcgccg acgtcgccgc caagacgaag ctgggccgtc cgctcaaggt
       61 tgtctgcgcc tgcggcaacg gcaccgccgg cgtgttcgcg ccacaggccc tgcgcctgat
      121 gggctgtgaa gtcatcgaga tggataccga tctcgactat accttcccca aatacaatcc
      181 gaacccggaa gaccacgtca tgctgatgca gatggcggcc aaggttcggg agacgggcgc
      241 cgatctggcg ctcggcttcg acggtgacgg cgaccgttgc ggcgtggtcg atgacacggg
      301 cgaggagatc ttcgccgaca agatcggcct gatgctcgct cgcgacctgt cggcgatcta
      361 caagaacgtg accttcgtcg tcgatgtgaa gtcgaccggc ctctacaaga ccgacgagat
      421 cctcaaggcc aacggcgcga cgaccgtcta ctggaagacg ggtcactcct atatcaagcg
      481 caagaccgca gaactgggcg ccctggccgg gttcgagaaa tcgggccact tcttcctgtc
      541 gggtccgctg ggccacggct acgacgacgg catcgtcgcc gccggcgccg tgctggcgat
      601 gctggaccgc aacgcgggca agacgctgtc ggagctgaag accgcattgc cggacgcctg
      661 gacgtcgctg acgatgtcgc cgcattgctc ggacgagacc aaatatcagg tgctcgaaaa
      721 gctggtcgcc gaatacgagg acttgttcgc ggctggcggc tcggtccttg gccgcaagat
      781 cgtcgaggtc atcacggtca atggcgcgcg cgtgcatctg gaagacggct cgtgggtcct
      841 gatccgcgcc tcgtccaaca agcccgagat ggtcgtcgtg gtcgagagca tgcggtccgc
      901 cgacgacatg cgcgacctgt tccgtcagga gatgaagccg cgcctcgccg cccacggcgt
      961 cgtcgaatac aatcaggaaa tctgatcagg ccggagtcgt ccgggggccg acagctccgt
     1021 ccccgacgtc tagatcgcgt tcggcagggt cagttcgaag gtcgtgccgc ccggaccggt
     1081 gtcgaccagc cgcaactcgc caccgtggtt ggccgccagt tcgcgcgaga tggtcaggcc
     1141 gagacccgtg ccgtccgagg ccttgccgct gacgaagggc tcgaacagtc gctccgacag
     1201 gcgctgcggg atgcccgggc cgtcgtcggc gatgcggatc acgaccactt cgccgtccgt
     1261 cagcgcctcg atccgcacca cgcccttgcc tttgcgatcg ccgcgcgtgg cgtcggcctc
     1321 aatggcctga cgggcgttgc gcatcaggtt gacgaggatg cggtgcaact ggtcgctgtc
     1381 ggcgttgatg gcgaagcgcg ccggtatgct cttggacagc cgcacgcccg cgggatccag
     1441 tcctgcgtcc tcggcggcgt tgctcgcggc ggtggctagc gggatgcgtg tcaggaccgg
     1501 cgcgggctct tccgacttgc catagtcgag cacgttgcgg gtcagggcgg cggcgcggct
     1561 cagcgcccgc tccagtcgag gcatggccat ggccacctgc gggtcggcgg acgacgccag
     1621 acgctcggac gccatctggg ctgaggtcag catgttccgc aggtcgtgat tgatcttggc
     1681 cacggcctcg cccagcgcca cgaggcgaga ccgcgcacgt agcgactgcc gcacctcgtc
     1741 ctgcatccgc gccaactcgc gctcgacccg gccgatttca tcgtgccgat ccgagggggt
     1801 aggggcgacg gtctcgacgt tggcggcgaa gccttcgatc gaccgcgtca cccgccgcaa
     1861 aggctgcagc accagaaacg ccagaccggc atagagcagc cccccggcga cgatggagat
     1921 caggatcgac accagcaggc tgttcagcag aaaggcgcgc agctccatcc tcagcggttc
     1981 ggccggcgcc accacgtcga tgaagtcgcc cgagcggtag cgcggcttgg cccggacgcg
     2041 gatcgaccgg tcgggatcgc ccatcagggt cactacgggg tcccacaggc gggcggtgag
     2101 gccgcggccc ctcaggtcga tgaagtcggg cgcacggggc agattggggg cctgcagcag
     2161 ctggcgcatc accccctgat cgctgatggc cacggccgag acgccgccga tgcgcagcat
     2221 gctttcggcg gtggtgtcgt ccacggcgtt gtagggcagg gcctctaccc cgacggaggc
     2281 cagttcagcg gcctgcagcc ggtcggtcag ccagcgctcg tggaacgagg cggcgctggg
     2341 cgcgatgatc aacgcttcga ccgcgagggt gaaggcgacg gtcagcagca gcagccgaga
     2401 cgacagaccg tccggcgcat gcggggtcag ccgctcgcgc cactcgggcg aaagacgcag
     2461 cttgccggca agatcgtccc ccagactcat atgcccgtct tagcagggat aacgctgccg
     2521 cgccgcctca tcgcgcgacc ttcgggacca gccacgcctg cgccgccttc agcgcgaacg
     2581 gcaggacgat ggccagcacg aacacgccca tcatcggcgc ccagaactgg ccgaacaggg
     2641 cctgaaaatc cggattgggg tcggtggcca gcagggtgtt caaccgggct cctatccagc
     2701 aatagatgat gtgcgcaggg agcaggccga tcacggtcgc cacggtgtag ggcagcaagc
     2761 gcacccccat aaccccggcg gcgatgttga tcatgtggaa cggcactacg accgcgagcc
     2821 ggctcgccag cacgtaccag aaggtctcct tgtcgatggc gaggcagacc ttctgcatca
     2881 ggcctgcgtt ctgctcggcc cgttcgcgca tcgacttccc aagcgccgag cgcgcgaccc
     2941 cgtagacgat cagggcaccg agggtcgcgg cgatcgaggt cgagaccccg ccgacccacg
     3001 gtccgaacag atagccagcg gtgatcgtca cgaagaacac ccccggcacc acgctcgcgg
     3061 tggccagggt gaagatggcc atcagcacca gaaggctgac gatccagtgg tcggccgtaa
     3121 aggcgcgcag ttctccacca tgcaggcgca gggtctccag cgacagataa cggttccatc
     3181 ccatgccgaa gatcagggcc atcagacagg ccagtatgat gaggggcgcg aaacgccgca
     3241 gacgattcat tcgaactcca acccttaggg gcgtgctaca agggctttgg cctgtcgaag
     3301 gcgttgacac cctccgacat gacacttata cgcccgccct ccgcaacgga tgcttttcag
     3361 tccgtcgcac aaccattcaa ccctaggagc cgaccgtgaa gcggacctat cagccgtcgc
     3421 gactcgtgcg caagcgccgt cacggcttcc gttcgcggat ggccaccaag aacggacaga
     3481 agattgttgc gcggcgtcgc gcaaagggtc gcaagaagct gactgcttaa gcgacctgaa
     3541 gttccttccc tcgtgggagg acctatgacg gatgttctga aaatcgaacg cctgacctcg
     3601 cggccccagt ttctggcggc cgcgaaaggc gtttctctgg cccgtggtgc ggtagtcgtt
     3661 caaaggctcg gcagggccga cggcgacccg gtcgtgcggg tgggtttcac tgcgacccgc
     3721 aaggtcggcg gcgccgtcgt ccgcaatcgg gccaagcgtc gtctgcgcga ggccgcgcgt
     3781 gctctcgtgc ccagccacgg cgtgcccggc agcgactatg tattgatcgc gcgcatgggc
     3841 acccccggcc gcgattggtt gcgtttgctt gacgacgtca aatcggcgct tacaaggctc
     3901 gcaacctcgc cggccccatc agtcgggaac tcgggcgata agcccaacgc cgccgcccgc
     3961 gacaccacca cccaggaccg ctgaccgaat gaagctcgat aacgcacgca acacggtgat
     4021 cttcatcgtc tgcacagtgg tgatcctgat cctgtatcag gtcttcgtcc tgcagcccca
     4081 ggccaactcc cgcaaggcgg cgctggcggc cgagagctcg cagccggtcc gtccagacgc
     4141 cagcgcggcg atcgggcccc agaccacccc gaccagcgga aacttcgtca ctgaccgcac
     4201 gcaggccctc ggcggcgcag cgcgcgtgcc gatctcgacc ccgaccctgc gcggctcgct
     4261 gtcgctgcaa ggcggtcgta tcgacgacct gttcctgacc aaataccgca ccaccatcaa
     4321 gccggacgct ggccttgagg aactgttccg cccgcagggc atggagcacg ccttcttcgc
     4381 ccagttcggc tggaccggcc ccaacattcc gggcggcgtt cccggcccga atacggtctg
     4441 gagcctgacc aacggttcga ccctgacgcc cgcaaccccg gtcaccctga cgtgggacaa
     4501 cggtcagggc ctgcgcttca cccgcatcgt ctcaatcgac gaccagtata tgttcgccgt
     4561 ttcggacacg gtcgccaacc tggggactca ggtcgtcacc atcgccccct atggccgcgt
     4621 cgagcgtcag ggcgtgccgc ccgcactggg taagacccag atcctgcacg agggcgccat
     4681 cggcaccttc agcaccgccg acggcaaagg ctattcgacc gaacagctca agtacaaggc
     4741 ttgggaaaag aagccgctcg aggcgcatac ttcaaaggga ggctggctcg gcatcaccga
     4801 caaatactgg atggcggccc tgatcccgcc ccagaccgag atcgtcactg cccagttcaa
     4861 ggtcgatgac gcccagcgtt tcgacatcca cacggcccag atgctcggcg cctcgcacac
     4921 gatccagccg ggccagacga tcaccgaaca acagcatttg ttcgccggcg ccaagcgcaa
     4981 tgaaatcctc gccggttacg agaagtcgct cggcctgccg cgcttcatct acgccatcga
     5041 ctggggcttc ctgttcttcc tgacgcgccc gatcttcttg ctggtcgagt tcttctacgg
     5101 ccttgtcggc aacttcggcg tggccatcct gctgctgacc ctgaccatca agctgatcat
     5161 gttcccgttg gccaacaaga gctacgagag cctgtcaaag atgcgtaagc tccagcccaa
     5221 gatggaggcg atcaaggcgg cccataagga cgacccggcc gcccaacagc gcgagaccat
     5281 ggcgctgtat cagtcggaaa agatcaatcc gctggcgggc tgtctgccca tcgtggtcca
     5341 gatcccggtc ttctatgcgc tctacaaggt gctctacgtc accatcgaga tgcggcacgc
     5401 gccgttcttc ggctggatcc gcgacctttc ggaccgcgac ccgaccaaca tctggaacct
     5461 gttcggcctg atcccgtggg atccggcgac cgcgcccctg atcggcaatt tcctgtcgcc
     5521 tcacgtgggc ggcggtttta ccctggcgct cagcgttctg gcgatcatct acggcctgac
     5581 catgtggttg cagcaggcca tgaacccgcc tgcgcccgat ccgatccagc gccagatctt
     5641 catgttcatg ccgctgatct tcaccttcat catggccgcc ttcccggcgg gcctgctggt
     5701 ctactgggcc tggaacaaca ttctctcgat cgtccagcag tatttcatca tgcacaggtt
     5761 caaggccgag aacccgatcg acaccttctt ctcgcgtctg aagaaggcca cctgagttgg
     5821 aatattctga cgaagagatc gaggccgcgc gggtcctgtt cgagcgccag gcgaccttcg
     5881 tcatgggggc tgcgaagatc gagcaactcc ccgaaccgaa cctgccggag atctgcttcg
     5941 ccgggcgttc gaacgtcggc aagtccagcc tgatcaacgg actggtgagc atgcacaagc
     6001 tggcccgcgc ctcgaacgag ccgggccgga cgcgcgaggt caatttcttc gacctcgacg
     6061 gcaaactgcg cctcgttgac ctgcccggct acggctgggc caaggcgtcg aagacgactg
     6121 tgaagaagtt ccaggacctc ggtcgcgatt acctgcgtgg ccgtgtgacg ctgaagcggg
     6181 tctatctgtt gatcgacgcc cgtcacgggc tgaaggcggt ggacggcgag gcgctcgagg
     6241 cgctggatct cgccgccgtc agctatcaga tcgtcctgac caaggccgac aagatcaagc
     6301 ccgccgaggg tctggccatg caggagaaga ccctcaaggc catctccaaa cgccccgcgg
     6361 ccttcccggc cgtcgccctg acctcagccg aaaaaggctt cggcatgccc gagctccgcg
     6421 ccgagatcat gcgcaccacg ggcgccgaaa tctaggaagc gcttttctgg cgtcgtgccc
     6481 gcgacggctc gctgaaatcg tgtagcttgc gtccacctga tacggagacg ctcgtgactg
     6541 accctgctca tcccgaaggc cctggcgtct ggaccctgct tcaggatcac gtcacccatg
     6601 tcatgtggca gcaggatgaa acggatggcg caggcgcaat ggccagtcgc ttctgggtca
     6661 tcaacccgcc tgaacagttg atgttcgaca ccttcgacga agccgaagcg atgttcgaac
     6721 agctcagtg
//
LOCUS       DASVTM010000145         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_439683,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000145 DASVTM010000000
VERSION     DASVTM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439683"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..588)
                     /locus_tag="VF633_04135"
     CDS             complement(124..588)
                     /locus_tag="VF633_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470280.1"
                     /translation="MPAPIQGMVAEWAAQGTLGLSPEDQFGEIFNSLLIPHELGHFVQ
                     MLDGRDRTLDNWSAEVDANRVAVAFWSLEPADAARIAERVANFNDFLAALPSPVPAGE
                     DPHAYFEANYERLGSDPAAYGWYQGAFMRTAWAQRQDATFCQLVKPGAASPS"
     gene            complement(912..2303)
                     /gene="fumC"
                     /locus_tag="VF633_04140"
     CDS             complement(912..2303)
                     /gene="fumC"
                     /locus_tag="VF633_04140"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX8470281.1"
                     /translation="MTDTRIETDTFGPIEVANDRYWGAQAQRSLGNFRIGWEKQPLPI
                     VRALGIVKRAAAETNMALGKLDATVGNAIIAAANEVIEGKLNDHFPLVVWQTGSGTQS
                     NMNANEVISNRAIEMLGGEMGTKKPVHPNDHVNMSQSSNDTYPTAMHVACAEQVVNDL
                     LPALKHLHAALKAKSEAWAHIIKIGRTHTQDATPLTLGQEFGGYTQQVENGIARIELT
                     LPALMELAQGGTAVGTGLNAPIGFAEKVAEQIAGITGLPFTTAPNKFEALAAHDAMVF
                     SHGAINTVAASLFKIANDIRFLGSGPRSGLGELALPENEPGSSIMPGKVNPTQCEALT
                     QVCVQVFGNNAALTFAGSQGHFELNVYNPVMAYNFLQSVRLVTDAAISFTDNCVVGIE
                     PRVENIERGLNNSLMLVTALNGKLGYDACAKIAKTAHKNGTTLRQEAVGGGYLTDAEF
                     DEAVRPEKMISPG"
     gene            2399..2701
                     /locus_tag="VF633_04145"
     CDS             2399..2701
                     /locus_tag="VF633_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX8470282.1"
                     /translation="MGVYSSQNSGEVLIMELGVFEARNRFSELVEAAERGEETIVLKH
                     GKAVAKLVPIDLVATRVANTMKALEMAQEFGARITARNGRRFTHEELMDAKTEGRR"
     gene            2706..3116
                     /locus_tag="VF633_04150"
     CDS             2706..3116
                     /locus_tag="VF633_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8470283.1"
                     /translation="MVVDASFLAAAIFEEDHTRFARDIIAKHPDGFTAPALLTWELSN
                     IVWKKCRRGLIDAADIPDFISIYATFDVALARLPDLDELGELTATAVKRDLTAYDAAY
                     FQFAVMQGFPLATTDRGLSKTARAAGVVVHSPFA"
     gene            3116..3472
                     /locus_tag="VF633_04155"
     CDS             3116..3472
                     /locus_tag="VF633_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEX8470284.1"
                     /translation="MDKTWIGTGKVRARETGEMVEITIDGLTTQAKYYKPLVYEFMRK
                     EWQSRPSYGDYVVEILMEHVGDAPWMDLDNLAKALLDAIKGYLFHDDSQVARLLVERR
                     EGERERITIRSFPRKD"
     gene            complement(3488..3982)
                     /locus_tag="VF633_04160"
     CDS             complement(3488..3982)
                     /locus_tag="VF633_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX8470285.1"
                     /translation="MAEDTPPIDEMNYEALAQDALRGVIRAALERAASPGGIPGEHHF
                     YITFKTRGVGVSVPPDVLAKYPDEMTIVLQHQYWDLAVEHDLFSVMLKFGGMPKVLTA
                     PYSAVTRFYDPSVQFLLQFESPELVSEPVTTMVPEPTRPAIVQSEGEDSGPKVVSLDQ
                     FRKK"
     gene            complement(4183..4563)
                     /gene="ssrA"
                     /locus_tag="VF633_04165"
     tmRNA           complement(4183..4563)
                     /gene="ssrA"
                     /locus_tag="VF633_04165"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            complement(4561..>4705)
                     /locus_tag="VF633_04170"
     CDS             complement(4561..>4705)
                     /locus_tag="VF633_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX8470286.1"
                     /translation="AGHLAGETTLEAAVDAARQATRNYAKRQLTWFRNQTQDWPRQSI
                     STS"
BASE COUNT          854 a         1475 c         1464 g          912 t
ORIGIN      
        1 cctgttagac cagcttcgga tcgataccga tgttcagttc gaccttgtcc atccgcaagt
       61 taacccgaaa ctcgccaaag cccaaccggg cgccgggcgt aggtcgggtt ccggagagcc
      121 cagtcacgaa ggtgaagctg cgcccggttt gaccagctgg cagaacgtcg cgtcttgccg
      181 ctgcgcccag gccgttcgca tgaaggcgcc ttgataccat ccgtaggctg cggggtcgct
      241 tcccaaacgc tcgtaattcg cctcgaaata ggcgtgggga tcttcgcctg ccggaaccgg
      301 gctcggaagt gcggccagga agtcgttgaa attggccacg cgctccgcga tgcgagctgc
      361 atctgcaggc tccaacgacc agaaggcgac ggctacgcga ttggcgtcca cctctgccga
      421 ccagttgtcc agcgtccgat ctcgcccgtc gagcatctgc acgaaatgcc cgagctcgtg
      481 cggaatcagc aggctgttga agatctctcc gaactggtct tcgggcgaca aacccagcgt
      541 gccttgggcg gcccattccg cgaccattcc ctgaatcgga gctggcatct ccgtccagcg
      601 ggatagatgg agcgaccggt cacgatgact gaacgacaca agggaaggct cggagtcgac
      661 gaccacaccg ggcatggctc cgggctctat tccgcaggct ttcagagccg tctcgaactt
      721 ctgaatcaag ggtgacgcca tgtccgcgac cgggtctggc tttatctgca cctcggccga
      781 cgcgtttgcg gcggacgtca acgttagcag cgaagccgcc gccaagcttg aaaacaggcg
      841 catcgacact acaaacctcc cggagaacgc cgatccttag aatgcgctcc gccagtcttc
      901 ttgtccagcc gtcaccccgg tgagatcatc ttctccggcc gcacagcctc gtcgaactcg
      961 gcatccgtca aataaccgcc gccgacagct tcctgacgca gggtcgtgcc gttcttgtgc
     1021 gcggtcttgg cgatcttggc gcacgcgtca tagccgagct tgccgttcag cgccgtcacc
     1081 agcatcagcg agttgttcag gccgcgctcg atattctcga cgcggggctc gatgccgacg
     1141 acgcagttgt cggtgaagct gatcgccgca tccgtcacca gccgcaccga ctgcaggaag
     1201 ttgtaggcca tcaccgggtt atagacgttg agttcgaaat gtccctgcga gccggcgaac
     1261 gtcagggcgg cgttgttgcc gaacacctgc acgcagacct gcgtcagcgc ctcgcactgg
     1321 gtcgggttga ccttgcccgg catgatcgac gagcccggct cgttctcggg cagcgccagc
     1381 tcacccagac ccgaacgcgg gccggaaccg aggaagcgga tgtcgttggc gatcttgaac
     1441 agcgatgcgg cgaccgtgtt gatggccccg tgactgaaga ccatcgcgtc gtgggcggcc
     1501 agcgcctcga acttgttcgg cgccgtcgtg aagggcaggc cggtgatgcc cgcgatctgc
     1561 tcggcgacct tctcggcgaa accgatcggc gcgttcaggc cggtgccgac ggccgtgccg
     1621 ccctgcgcca gttccatcag ggccggcagc gtcagttcga tccgcgcgat tccgttctcg
     1681 acctgctgcg tataaccgcc gaactcctga ccgagcgtca ggggcgtagc gtcctgagta
     1741 tgcgtccggc cgatcttgat gatgtgcgcc caggcttccg acttggcctt cagcgcggcg
     1801 tgcagatgct tcagcgcggg cagcaggtcg ttgaccacct gttcggcaca ggcgacgtgc
     1861 atggctgtcg gataggtgtc gttcgacgac tggctcatgt tgacgtggtc gttcgggtgg
     1921 accggcttct tggtccccat ctcgccgccc agcatctcga tggcgcggtt cgagatgacc
     1981 tcgttggcgt tcatgttcga ctgggtgccc gacccggtct gccagaccac cagcggaaaa
     2041 tggtcattga gcttgccttc gatcacttca ttggcggcgg cgatgatcgc gttaccgacc
     2101 gttgcgtcga gcttgcccag cgccatgttc gtctcggcgg cggcgcgctt gacgatgccc
     2161 agagcccgca cgatcggaag cggttgcttc tcccaaccga tcctgaaatt ccccagcgac
     2221 cgctgcgcct gcgcgcccca gtatcggtcg ttggcgacct cgatcgggcc aaaggtgtcg
     2281 gtttcaatgc gtgtgtcggt catgcgcagc actcgtcttc aggctcggcg ggagaatggc
     2341 ggcgggttta gcacgggcgg gcgagggggc aaggcggcgc gacgttgctg tgccggacat
     2401 gggggtctac tctagccaga attctggcga ggtgctcatc atggaactcg gcgtcttcga
     2461 agcccgcaac cgcttctccg aactggtcga agccgccgag cgtggcgagg aaaccatcgt
     2521 cctcaagcac ggcaaggctg tcgccaagct ggtgccgatc gatctcgtcg ctactcgcgt
     2581 cgccaacacg atgaaggcgc tggagatggc gcaggaattt ggtgcgcgta tcaccgctcg
     2641 aaacggacgc cgattcacgc atgaagagtt gatggacgcc aagaccgaag gccgtcgctg
     2701 aagtgatggt cgtcgacgct tcgtttttgg ctgccgcgat cttcgaggaa gaccacacgc
     2761 gcttcgcccg ggacattatc gctaaacatc cggatgggtt cacggcgcca gctctcctga
     2821 cttgggagtt gtccaatatc gtctggaaaa aatgccgacg cggtctgatc gacgccgccg
     2881 acatccccga tttcatttcg atctatgcaa ctttcgacgt cgcgctcgca aggttgccag
     2941 acctcgacga gcttggtgag ttgaccgcga cggccgtgaa acgcgatttg acagcctacg
     3001 acgcggccta tttccagttt gccgttatgc agggctttcc tttggccact actgaccgcg
     3061 gcctgagcaa gaccgcacgc gccgccggtg tcgtcgtcca ctcgccgttc gcctgatgga
     3121 caagacctgg atcgggaccg gcaaggtccg tgcccgcgag accggcgaga tggtcgagat
     3181 caccatcgac ggcctgacca cacaggcgaa atactacaag ccgctggtct atgagttcat
     3241 gcgcaaggag tggcagtcgc ggccgtccta cggcgactat gtcgtcgaga tcctgatgga
     3301 acacgtcggc gacgccccgt ggatggacct cgacaatctt gccaaggccc tgctggacgc
     3361 catcaagggt tatctgttcc acgacgacag tcaggtcgcg cgtctgctgg tcgaacgccg
     3421 cgagggcgaa cgtgaacgta tcaccatccg cagcttcccc cggaaagact agacgctcgc
     3481 ccgctgtcta tttcttgcgg aactggtcca gcgacacgac cttgggtcct gaatcctcgc
     3541 cttcggactg cacgatggcc ggacgcgtgg gttccgggac catggtcgtc accggttcgg
     3601 acaccagctc cggcgactcg aactgcagca ggaactgcac gctcggatca tagaaccggg
     3661 tcacggcgct gtagggcgcg gtcagcacct tgggcatgcc gccgaacttc agcatcacgg
     3721 agaacaggtc gtgctcgacg gccagatccc agtactggtg ctgcaggacg atggtcatct
     3781 cgtccggata tttggccagc acgtccggcg gtacgctgac gccgacgccg cgcgttttga
     3841 acgtgatgta gaaatggtgc tcgccgggaa tgccgccggg gctggcggcg cgttcgagcg
     3901 ccgcacggat caccccacga agggcgtcct gcgccagcgc ctcgtagttc atctcgtcga
     3961 tcgggggggt gtcttccgcc atctgatcct cagcgcgcca gtagcgtgac tgatagcggc
     4021 gtcgggctcc cgacgaaagg ccgtatgacg gatatgttct gaggaaattg gagagctatg
     4081 cggcctcaac cgtctgctca tcctggaata ctgccaaggg ctctgttggc gagaccccgc
     4141 caatcttcca aagcaagaag ccgggcactt aatgataggg aaagtgccgg ggattctgtt
     4201 ggcaggctcc ccggcggccc cgcctaagga tctgaacccc taggacttaa gattcgaaat
     4261 caaccgaacc gcattacgcg gcgaggcgga cttctccagc gaagttatcg ttcgcagtta
     4321 caaaatggcc cgatacggtg ggccaggacg ggcaaaagca gtcttcttta cacgtccgtc
     4381 gatgctagtc ggccccccat ttccaacctt ggatcccgcc gtcgaaaaca cggtgagagg
     4441 ttggtggagc cgccgggaat cgcacccggg tccggtccgc ttattacaag cgcgtttatc
     4501 gccatatccg gagcaagccc cggcgggatc aatataggcg caggcgacgc cgcttccaag
     4561 ttacgatgtg gaaattgatt gccgcggcca gtcctgtgtc tggttcctga accacgtcag
     4621 ctgccgtttg gcgtagttgc gggtcgcttg gcgggcggcg tcgacggcgg cttccagcgt
     4681 cgtctcgccc gccagatggc cggcg
//
LOCUS       DASVTM010000146         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_652833,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000146 DASVTM010000000
VERSION     DASVTM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652833"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VF633_04175"
     CDS             <1..792
                     /locus_tag="VF633_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX8470287.1"
                     /translation="TLAAFAPPPRLVKAERAGAPTHVAPGETEVTGFVFKVQANMDPN
                     HRDRIAMFRMASGEFKRGMKLKVQNTGKQLSVNAPIMFFASDRELADDAFAGDVIGIP
                     NHGVLRVGDSLSESGMIRFAGLPNFAPEILQRVRVKDPLKAKHLKKALDGLAEEGVTQ
                     LFRPEMGADFIVGAVGQLQFEVMADRLGEEYGLDVIFEHSPYAEARWVDGADADVEDF
                     SSKYRGQMARDIDQAPVFLAKSSWETGYVTERFPKVAFTKTKERG"
     gene            933..1520
                     /locus_tag="VF633_04180"
     CDS             933..1520
                     /locus_tag="VF633_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470288.1"
                     /translation="MASGAMARQTPTGETPAQARARAAASALNQGVTESPTHSEQAAR
                     PARTQPIQTRAPLSPDPVPTSGPSTAASPEATPPEPDGLPRGYYARADKDCNQVWPGD
                     GELAWLSETAFTIDFGGCEPGLIQQLGPTTWHEEQRCVTELGGDGGAYSIDYELTGPG
                     EILRTARLAVDQSVESQTWKHCDTADVPAEARFRS"
     gene            1708..>2267
                     /locus_tag="VF633_04185"
     CDS             1708..>2267
                     /locus_tag="VF633_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470289.1"
                     /translation="MALTALTPMSAMAEWRRAESEHFVVYSDGSDRSLRDYTAKLERF
                     DQLLTSRFGGARAEARRLPVYLVGDAKALRVAVPGLPAGIAGYYSSSNTDVFAVLVRG
                     ESDDILLHEYSHHVMARAGDMRYPGWFSEGFAEYFATATVTERGSASFGLPNPGRLQA
                     LQTERWLPMDVVLRAESSLGVGSETGR"
BASE COUNT          412 a          747 c          726 g          382 t
ORIGIN      
        1 accctggcgg ccttcgcgcc gccgccgcgg ctggtcaagg ccgagcgcgc cggcgcgccc
       61 acccacgtcg cgcccggcga gaccgaagtc accggcttcg tcttcaaggt ccaggcgaac
      121 atggacccca accaccgcga ccggatcgcc atgttccgca tggcctcggg cgagttcaag
      181 cggggcatga agctgaaggt ccagaacacg ggcaagcagc tgtcggtcaa cgccccgatc
      241 atgttcttcg cctccgatcg cgagctggcc gacgatgcct tcgccggcga tgtcatcggc
      301 atccccaacc acggcgtcct gcgcgtcggc gacagcctgt ccgagagcgg catgatccgt
      361 ttcgccggcc tgccgaactt cgcgcccgaa atcctgcagc gcgtccgggt caaggatccg
      421 ctcaaggcca aacacctcaa gaaggcgctg gatggtctgg ccgaagaagg cgtgacccag
      481 ctgttccgtc ccgaaatggg cgcggacttc atcgtcggcg ccgtcggaca gctgcagttc
      541 gaggtcatgg ccgatcggct tggcgaagaa tacggtctgg acgtcatctt cgagcacagc
      601 ccctacgcag aggcccgctg ggtggacggt gcggacgccg acgtcgagga tttcagctcc
      661 aaatatcgcg gccagatggc ccgcgacatc gatcaggcgc cggtgttcct ggccaagtcg
      721 agctgggaaa ccggctacgt caccgaacgc ttccccaagg tggccttcac caagacgaag
      781 gaacggggct agttccactc ggggtcctcc tcgaccagcg acggttgctg cggggacaag
      841 gttctgtcca agagctgtgc tctgctgtcc ttatgaccaa ccgagacaga tcacgcgctt
      901 tggcgctcgt cgcgatcacc agcgggcttt tcatggccag cggcgctatg gcgcgccaga
      961 ctccaacggg tgagactccc gcgcaggcgc gggcccgcgc tgccgcttcc gcattgaatc
     1021 agggcgtcac ggaatcgccg acccattcag aacaggcggc tcgcccggcg cgcacccagc
     1081 caatccagac ccgtgccccg ctttcgccgg acccggtccc aacttcaggt ccctcgacgg
     1141 cggcctcgcc ggaagcgaca ccaccagagc cggacgggct tccgcgaggt tactatgcgc
     1201 gcgccgacaa ggactgcaat caggtctggc cgggcgacgg cgaactggcc tggttgtccg
     1261 aaacggcgtt tacgatcgac ttcggcggct gcgaacccgg gctgatccaa cagcttggcc
     1321 cgaccacctg gcatgaagaa caacgctgcg tgactgagct cggaggcgac ggcggcgcct
     1381 acagcatcga ctatgagctg accggccccg gcgagatcct ccgaacggcg cgcctggctg
     1441 tcgatcagtc cgtcgagagc cagacctgga agcattgcga caccgcagac gtgccggcag
     1501 aagcgcgttt tcggtcctga ccccggctat gtcaccgaac atttcgccga ggtggcgttc
     1561 acgaagagga agtaacggga ctaacggacg cgcagattag gtaaaatcag ggaaagttgg
     1621 accacgccac gtaaatcggg tttagcattt gcgggctctc gggggaggtc cattttgtcg
     1681 ccaaccgtcc aacgcctttt cttcgccttg gcgctgaccg ccctaacgcc gatgtccgct
     1741 atggcggagt ggcgacgcgc cgaaagcgag catttcgtcg tctatagcga tggctcagac
     1801 cgctcgttgc gcgactatac ggccaagctc gagcgctttg accaactcct gacgtcgcgc
     1861 ttcggcggcg cacgtgcgga ggcccgccgg ttgccggtct atctcgtagg cgatgcaaag
     1921 gcgttgcggg tggccgtgcc gggccttccc gccggaattg ccggctacta ctccagcagc
     1981 aacaccgacg ttttcgccgt tctggtgcgg ggcgaaagcg acgacatttt gctgcacgaa
     2041 tacagccatc acgtcatggc ccgggctggc gacatgcgat acccgggctg gttcagcgag
     2101 ggtttcgccg agtatttcgc tacggcgacg gtgacagaga ggggttcggc ttcgttcgga
     2161 ctgccgaatc ccgggcgcct gcaagcgctg caaaccgagc gctggttgcc catggacgtc
     2221 gtgctgcggg cagagagctc gctcggcgtc ggcagcgaga caggacg
//
LOCUS       DASVTM010000147         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_814436,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000147 DASVTM010000000
VERSION     DASVTM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814436"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VF633_04190"
     CDS             <1..520
                     /locus_tag="VF633_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8470290.1"
                     /translation="LHVVGDAMLEQVLSQMEALAPAAQWRPLRVRIEHANGLAGARLS
                     RAAALGLVVAQPRPTSPIASWIKAGIPVAYGSDSGFPPFAAFAQMVDPTNPNGVDRDT
                     ALAILTSGGAFAEFAEGDRGILDVGMQADLAVLSQDIMTVPGAALPATRSLMTIVAGQ
                     VAFEAPGFSSDP"
     gene            complement(684..869)
                     /locus_tag="VF633_04195"
     CDS             complement(684..869)
                     /locus_tag="VF633_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470291.1"
                     /translation="MADQPGSNRAVFKLKAGKVSMAGKLEVSTAGLFAIGVMVSSILG
                     SAAGVVWVATSVARKRL"
     gene            1050..1244
                     /locus_tag="VF633_04200"
     CDS             1050..1244
                     /locus_tag="VF633_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470292.1"
                     /translation="MSILDQVVKTVETAPLSGKLREIEAILERVSDPGDELDQDAALR
                     AIAAIAGSSRSTDIYQKPLG"
     gene            complement(1241..2200)
                     /locus_tag="VF633_04205"
     CDS             complement(1241..2200)
                     /locus_tag="VF633_04205"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8470293.1"
                     /translation="MTGPLPATPEADDALLGLLEWLEAAGYAFVNPTPSTHALVRERC
                     DARKEDLLRDVFGWVRPFDRAQMPDRLLALMETAGLLVDTEGKLASALRVSRLEGRLF
                     LHSAPTCDNDAVFLGPDSYRFARLLRQVLTHGPAIGHALDIGTGAGVGAVMVKDLAPD
                     AEVHASDINPSALRLAGLNARHAGLNVLTRLGSGLPGTPASFDLIIANPPYIAGDVGK
                     TYRDGGDDYGAALALEWVREGVRRLAPGGRFILYSGAAVVAGRDVVLESLTALCAHAG
                     LSLDYDELDPDVFGQTLRQNAYRNVDRIAAVGAVITAPSSRGG"
     gene            complement(2197..3111)
                     /locus_tag="VF633_04210"
     CDS             complement(2197..3111)
                     /locus_tag="VF633_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8470294.1"
                     /translation="MITPFHHGQSTAEFPDRRSFHQRLARWNNRRLAPGTPTNDWRAQ
                     AAEDAEMILIEGAFIERSRAEVSPLLADLPSDVDSFIAWFEALKGSGPGEGDPLFPWL
                     AETADFEQMRWFLLQEVAGEAGFEDLVAMAQVKMPTRPKLELARNYWDEMGRGSEHGM
                     HGPMLQRLADALDLQPTIEDTVTPSLALGNTLVAFSTTRRYAFHALGALGAVELTAPW
                     RAGHVAAGLKRLGVGSERKYFALHATLDVEHSRTWNEEVLRPLAETHPECIRSLAEGA
                     VMRLAAGARCYEAYRARLWEAAPVLRSA"
BASE COUNT          623 a         1109 c         1116 g          590 t
ORIGIN      
        1 tctgcacgtc gtcggtgacg ccatgcttga gcaggtgctt tcccagatgg aggcgctggc
       61 gccggcggcg caatggcgcc cgctgcgggt gcgcatcgaa cacgccaacg gcttggcggg
      121 cgcccggctg tcgcgagcgg cggcccttgg actggtggtc gcgcagcctc gaccgacctc
      181 gcccatagcg agctggatca aggcggggat cccggtcgcc tatggctctg actccgggtt
      241 tcctcccttc gcggccttcg cccagatggt ggatccgacc aatcccaacg gggtggaccg
      301 cgatacagcg ctcgccattc tgacaagcgg cggtgcattc gccgagttcg ccgaaggtga
      361 cagaggcatt ctcgacgtcg gcatgcaggc ggaccttgcc gtcctctccc aagatatcat
      421 gacagtaccc ggagccgcgc tgcccgcgac ccgcagcctg atgacgatcg tggccggcca
      481 agtcgcgttc gaggcccctg gattttctag cgatccctga ccgttctttg gccgaccagc
      541 ctacgtcatg gccgccttgg tggagccgcg actgcaacag cggggaatcg cgatttgccg
      601 ctactgcagt cttgaaagcc gtcttcggat gcgtcgcact gtcttggccc cgtccgccgc
      661 tgagccgctc agtgacgccg ttactagagg cgctttcgtg cgacggatgt ggccacccag
      721 acgactccgg cggccgaacc aaggatgctc gataccataa cgccgatggc gaacagcccg
      781 gcggtggaga cctccagctt ccctgccatc gaaacttttc cggccttgag tttgaagacc
      841 gctctatttg aaccgggctg atcggccatg tcggttggct ccctccaagt ggagaccgaa
      901 ctgaccacga ccagatgggt tccggcgggg cgaccagggg aggtcgtgtc gccgtccgaa
      961 ggcgagcgcc tccgcggtct gggctcatat gctccctcgg acgcggatca tcatcgctgc
     1021 cagtgcgttg gatgaaaagg agcgacatca tgagcattct ggaccaggtc gtgaagaccg
     1081 tcgaaacggc gcccctgagt ggcaagctcc gtgagatcga ggcgattctt gagcgcgtta
     1141 gcgacccggg agacgagctt gatcaggacg cggcgcttcg agccatcgcc gccatcgccg
     1201 gctcatcccg ttcgacggac atctaccaga agccgctcgg ctagccgccc cgggagctcg
     1261 gcgccgtaat gacggcgccg actgcggcga tccgatcgac gtttcgatag gcattctggc
     1321 gcagtgtttg accgaacacg tcggggtcaa gttcgtcata gtcgagagac agtccggcgt
     1381 gcgcgcacaa agcagtcaga gactcgagaa ccacgtcccg gccggccacc acagcggcac
     1441 ctgaatagag gatgaaacga ccgcctgggg ccagccgtcg tacgccctct cgtacccatt
     1501 ccagagccaa ggcggcgcca tagtcgtcac cgccgtctcg ataggtcttg ccgacgtccc
     1561 cggcgatata gggagggttg gcgatgatca gatcgaacga ggcgggtgtc ccgggcaagc
     1621 cgctgccgag ccgggtcagg acattgaggc cagcatgccg tgcattcaat cctgctagcc
     1681 gcagggcaga ggggttgatg tccgaggcat ggacctcggc atcgggcgcc agatccttga
     1741 ccatcactgc gcccaccccg gccccggtgc cgatgtcgag cgcatggccg atcgccggac
     1801 catgggtcaa cacctgacgc agcagccggg cgaaccgata gctgtccgga cccaggaaga
     1861 ctgcgtcgtt gtcgcacgtc ggcgcggaat gaaggaacag ccggccttcc aggcggctga
     1921 cgcgcagcgc gctcgccaac ttgccctccg tatccaccag aaggccggca gtctccatca
     1981 gcgccagcaa gcggtccggc atctgagctc ggtcaaacgg acgcacccag ccgaagacgt
     2041 cccgcagcag atcctccttg cgagcgtcac agcgctcacg caccagtgcg tgtgtcgagg
     2101 gggttgggtt gacgaaggca tagccagcgg cttcgagcca ttcaagaaga ccaaggagcg
     2161 cgtcgtcggc ttccggcgtc gcgggaaggg gtcctgtcac gcggagcgaa ggacgggagc
     2221 cgcctcccag agacgggccc gataagcctc ataacagcgc gcacccgcag ccagccgcat
     2281 cactgcgccc tcagcaaggc ttcggataca ttcggggtgg gtctccgcga gcgggcgcag
     2341 cacttcctcg ttccaggttc gcgagtgttc gacgtccagg gtcgcgtgca gggcgaaata
     2401 tttgcgctcg gacccgacgc ccagacgttt caggcccgcg gcgacatgcc cggcgcgcca
     2461 gggagccgtc aactcgaccg cgcccaaggc gcccagggcg tggaaagcat atcgtcgcgt
     2521 tgtggaaaag gcgaccaaag tgttcccgag cgccagcgat ggcgtgacgg tgtcctcgat
     2581 cgtcggttgg agatccaggg cgtccgcaag ccgctgcagc atcggaccat gcatgccgtg
     2641 ttcggaaccc cggcccatct cgtcccagta gttacgcgcc agctcgagct tggggcgtgt
     2701 cggcatcttc acctgagcca tggcgacaag atcttcgaaa ccggcctcgc cggccacttc
     2761 ctgcagcagg aaccaacgca tctgttcgaa atcagccgtt tcggcaagcc acggaaacag
     2821 cgggtcgccc tctccgggcc cgctgccttt cagggcctcg aaccaggcga tgaaactgtc
     2881 aacgtccgag ggcagatcgg ccagcaatgg gctgacctcg gctcgggacc gttcgatgaa
     2941 ggctccctct atcaagatca tctccgcgtc ttcagcggct tgggcacgcc agtcgttcgt
     3001 aggcgttcca ggcgcgagtc ggcgattgtt ccagcgggcc agccgctgat ggaacgacct
     3061 gcgatccggg aattcggccg tggattgtcc gtggtggaac ggggtgatca tgacgggctc
     3121 ctgatctttg cggacaccga atacgcgcat ggaccatccc cggttccacg gccacaattc
     3181 tgaatggtct aggtcgcctg ggcgactctg gaacccgtga aggtgcttgg ggtttcgggt
     3241 atcctgcagg gaggagaccc gatgaatgac acgggattca actggtccat cgcgaccgcc
     3301 gatgacggct ggatatggat gctgaaggac cgcgacagcg ctcagattct ggttgcgggc
     3361 cgggctccga cgcgagcggt cgcagcggca atggtcgtgc gcgccatcgc gcgcggcatg
     3421 actgcggaat gctcgcag
//
LOCUS       DASVTM010000148         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_332633,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000148 DASVTM010000000
VERSION     DASVTM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332633"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /gene="pelA"
                     /locus_tag="VF633_04215"
     CDS             complement(<1..1097)
                     /gene="pelA"
                     /locus_tag="VF633_04215"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX8470295.1"
                     /translation="MRRQLKSGLAASTVIAALLCGALAAPAMAEVIGSNVPAVSMTAD
                     RIASLPAAEQDAWYDYLNRSIAQRALDRATLAAELPAGATPPPPPLAVGGGQHNMPLD
                     RDAAWYATPEARATADAIVSFQTPAGGWSKNQNRTIARLPGQRYANDAEHMELDMANF
                     DAPVDRFWTFVGTLDNDATTTEMRFLAKVAAQAQGRQGDAWRASIVKGVNYLLNAQYP
                     NGGWPQIWPLEGGFHDAITFNDNAVAKASMILEDVAESKEGFGFVPAALKVRAAAAQV
                     RAVDVILAAQLRINGQLTAWPQQVDPVTLQPSSARNYEPHSLASGETTDVLLFLMRQP
                     NPSAEVKTAVRAGIAWLEKTRVYDKSFEMTD"
     gene            complement(1219..>1957)
                     /locus_tag="VF633_04220"
     CDS             complement(1219..>1957)
                     /locus_tag="VF633_04220"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEX8470296.1"
                     /translation="DRGFMKDALERAKAAGVKTLVFTVDMPVPGARYRDAHSGMSGPN
                     AELRRMVQAMTHPGWALDVGLRGRPHDLGNVSHYLGKATGLADYIGWLGANFDPSISW
                     KDLQWIRDFWDGPMIIKGILDPQDARDAVSFGADGIVVSNHGGRQLDGVLSSARALSA
                     IADEVKGEITILADSGVRSGLDVVRLLALGADSVMIGRAFIYALAARGQGGVEHVLDL
                     LAAEMRVAMALSGCTRVSDINGNMIAN"
BASE COUNT          336 a          639 c          646 g          336 t
ORIGIN      
        1 tcgtcggtca tctcgaacga cttgtcgtat acgcgggtct tctcgagcca ggcgatcccg
       61 gcccgaaccg cagtcttcac ttcagccgac gggttgggct ggcgcatcag gaacagcagg
      121 acgtcggtcg tctcgccgct ggcgagcgaa tgcggctcat agttgcgggc gctggaaggc
      181 tggagggtta cgggatcgac ctgctggggc caggcggtca gttgaccgtt gatgcgcaac
      241 tgggcggcga ggatcacgtc gaccgcacgc acctgagccg ccgccgcacg aaccttcagg
      301 gctgccggca cgaagccgaa gccttccttg ctctcggcga catcctcgag gatcatcgac
      361 gccttggcga cggcgttgtc gttgaaggtg atggcatcgt ggaagccacc ttcgagcggc
      421 cagatctgcg gccagccgcc gttgggatac tgggcgttca gcaggtagtt gacgcccttg
      481 acgatgctgg cgcgccaggc gtcgccctga cgtccctgcg cctgagcggc gaccttggcg
      541 aggaagcgca tctcggtcgt ggtggcgtca ttgtcgaggg tgccgacgaa ggtccagaag
      601 cggtcgaccg gagcgtcgaa attcgccatg tcgagctcca tatgctcggc gtcatttgcg
      661 tatcgctgac cgggcaggcg agcgatggtc cggttctggt tcttgctcca gccaccggcc
      721 ggggtctgga agctgacgat ggcgtcggcg gtcgcgcggg cttcaggcgt cgcataccag
      781 gcggcgtcgc ggtccagagg catgttgtgc tgaccaccgc ccacggccag cggcggcgga
      841 gggggtgtgg cgcctgcggg aagctcggcg gccagggtcg cgcgatcgag cgcgcgttgg
      901 gcgatggagc ggttgagata gtcgtaccac gcgtcctgct cggcggcagg cagtgaggcg
      961 atacggtccg ctgtcatcga gaccgccgga acattgctcc cgatcacctc ggccatggca
     1021 ggcgcggcca gcgctccaca caggagcgcg gcgatgacgg tggacgcggc gaggccgctc
     1081 ttcagttgac gtcgcatggt gtcctcccgg gacagttttc tcattatatt ggtatcgatg
     1141 ctacatgatg agaacagccg gtcaatcgac tcaaacggtc acgcgtgtcc gggcgctcaa
     1201 ctcacgtaac cgaggccgct agttggcgat catgttgcca ttgatgtccg acacgcgcgt
     1261 gcagcccgaa agcgccatag cgacccgcat ctcggcggcc aacaggtcga ggacatgctc
     1321 gacgccaccc tgccctcgcg ccgccaaggc atagatgaag gcgcgaccga tcatcacgct
     1381 gtccgcacca agagccagca accggacgac atccagcccg ctgcggacac ctgaatcggc
     1441 caggatcgtg atctcgccct tcacctcatc agcgatggcg gacagcgctc gcgccgacga
     1501 cagaaccccg tccagctggc gcccgccgtg gttcgagacg acgatcccgt cggctccaaa
     1561 gctgaccgcg tcacgggcgt cctgcggatc gaggatgccc ttgatgatca tcgggccgtc
     1621 ccagaaatcg cggatccact gcaggtcctt ccacgagatc gagggatcga agttggcgcc
     1681 caaccagccg atatagtccg cgagccccgt cgccttgccg agataatgcg agacattgcc
     1741 gaggtcgtgc ggcctgcctc gcagtcctac atccagcgcc caacccggat gggtcatcgc
     1801 ctgcaccatc cgccgcagct cggcgttcgg cccgctcatg ccggagtggg catcgcgata
     1861 ccgggcccct gggacgggca tgtcgacggt gaagacaagc gtcttcacac ccgccgcctt
     1921 cgcccgctcc aacgcatcct tcatgaagcc ccggtcg
//
LOCUS       DASVTM010000149         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_600300,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000149 DASVTM010000000
VERSION     DASVTM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600300"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF633_04225"
     CDS             complement(<1..216)
                     /locus_tag="VF633_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470297.1"
                     /translation="MTTATLSFRNLGSRQTWEARNLEFRFDIHIPRGRSSVGGLQMTV
                     LVTGRQASAGSSPLIDPFGRTIDYVRVS"
     gene            571..1299
                     /locus_tag="VF633_04230"
     CDS             571..1299
                     /locus_tag="VF633_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8470298.1"
                     /translation="MSFTAHSSPGVVSYSAGNSSFGRAEGFAAEHPIIRALPPEAVNC
                     IELHGIRVTEPARTPLDNATPHARFVVSGAIGVFPAPDSACVAMIGPGGLQGLEHCFL
                     ESISERHVVLVDAEWIEVPAAALALAMGKQWAERMFAYQASGRLRLLGAEAACNARHP
                     VSQRLARWLLRLHEAAGSPRRLEITQSLLGEILGVQRTTVNAAVGYLHQVGVVDNTRG
                     RITIPNRDALSLASCGCGDAPRGG"
     gene            complement(1648..>2953)
                     /locus_tag="VF633_04235"
     CDS             complement(1648..>2953)
                     /locus_tag="VF633_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470299.1"
                     /translation="DRIDDLEQIAALSIRTLRYPMVWERIETKPGRFDWSWMDRRMER
                     LRALGIRPIVGLIHHGSGPRWTNLLDDGFAPGLARFAGEAARRYPWVQDWTPVNEPLT
                     TARFSALYGHWYPHAVSEGDCWRALLNQVDATRLAMSAIRAVIPGARLVQTEDFGITF
                     GTAACLEQVEHENIRRLATWDLLAGQVGEGHPLYDRISSFGFGDRLASVAKSPCPADV
                     IGLNHYATSDRFLDHRLALYPPDLHGGNGLMAYVDTEAVRVDHGGQGGWVEMMRQLWA
                     RYATPIAVTECHLGCSVDEQIRWLGDCWSAALSARDEGIPVEAVTAWALLGSFDWNSL
                     LTRQVGYYEPGAFDLSGGRGPVITPLGEAISQLANGGWIDPHAVGAPIVERGWWTRAD
                     RFGDVATEDGYDEMIVEGATITEHGTSRNGRHSLGEVLRSLS"
BASE COUNT          536 a          966 c          938 g          513 t
ORIGIN      
        1 ggagactcgt acgtaatcga tcgtccgacc gaaggggtca attagcggcg acgagccagc
       61 cgacgcttgc ctgccggtga ccagcaccgt catctgcaga ccgccgactg aggaccggcc
      121 cctggggata tgaatatcga atctgaactc aaggttccgg gcttcccagg tctggcgcga
      181 gcccaggttc cgaaaggaca gggtcgcggt cgtcacgcta cggtccaacg ctgcggtgca
      241 gaggcagtcc aaagacgtga ggccatctcg taagcccccc gagatgtttg tcaaaacttc
      301 ccgggatagc cggcgctacg gccggactta acggccgggt cgcaagcaca cattgcggtg
      361 ggacagttcg atcgttcggg aaggcgccgg ttcggaggcg ctgcttcgtc ggcgcgtttt
      421 cttcctttga cgaacgggcc cactcgaagg accgaagccg ttgcggacaa gctggaccgg
      481 agccaggcca gtctggccac cagatccaaa ttctggcggg accacaacat atgtgaaagg
      541 cgaaaaccgc caagcctgtc actcaggagc atgtctttca ccgcacacag cagtcccggc
      601 gtcgtcagct attcggccgg aaatagcagt ttcgggcgcg cggagggctt cgccgccgag
      661 cacccgatca tccgcgccct cccgccggag gccgttaact gcatcgagct tcatggcata
      721 cgcgtcaccg agccggcgcg tacgcccctc gataatgcga cgccgcacgc aagattcgtg
      781 gtgtccggag cgatcggggt attcccggct ccggacagcg cttgcgtggc catgattggt
      841 cccggaggct tgcaggggct ggaacactgt ttcctggagt ccatcagcga gcgtcatgtc
      901 gttctggtcg acgcggagtg gatcgaagtc ccggcggctg ccttggcgct cgcgatggga
      961 aaacaatggg ccgagcggat gttcgcctat caggccagtg gtcggttgcg ccttctgggc
     1021 gccgaagcgg cctgcaacgc ccgacacccc gtctcacagc ggctcgcgcg ctggcttctt
     1081 cgtctccatg aagccgcagg ttcgcctcgg cgactggaga tcacccagtc ccttctcggt
     1141 gagattctcg gcgtccagcg cacgacggtc aacgcagcgg tgggctatct ccatcaggtc
     1201 ggagtagtcg acaacacgcg gggacggatc accatcccca accgggacgc cctgagcctg
     1261 gcctcctgtg gttgcggcga cgcgccccgg ggcggctgat ctcgccttgt cgctgagttc
     1321 aaggcaccgg cccggtcagt cgtgcaggct tccccgtcgt cgagcgtaga caagccggcc
     1381 ggcggcccag acctccacgc cctgacgccc cggcattcgc aaaagctctc cagcggcccg
     1441 ggcgacagct tcccgatcgc cggacagcac cgcctcttca gaaacgggcc gatcgcctga
     1501 ggcgataaag tacctgaatt cgtaggtcat cgtatcaact gaacagggcc gggcggggcc
     1561 tgaaatgtga cgtcgttgca gatttggatg gggacagctg gcccctgggc gaacagatgc
     1621 tcgggcgtca agcgtgcggc ggccgactca agagaggctc ctcaggacct cgccgaggga
     1681 atgacgcccg ttacggcttg tcccatgttc tgttatcgtt gctccttcta cgatcatttc
     1741 gtcgtatcca tcctcagtgg cgacgtcgcc gaacctgtcg gcgcgcgtcc accagccgcg
     1801 ttcgacgatg ggtgcgccca ccgcatgcgg gtcgatccag ccgccgttcg ccaactggct
     1861 gatcgcttcg ccaagcggtg tgatgaccgg cccgcgcccc cccgacagat cgaaggcgcc
     1921 gggctcgtag tacccgacct gtcgcgtcag aagactgttc cagtcgaaac tgcccagaag
     1981 ggcccaagcg gtgaccgcct cgaccgggat gccctcgtcg cgagccgaca gtgcggcact
     2041 ccagcaatcg ccgagccagc gaatctgttc atccacgctg cagccaaggt ggcattcggt
     2101 cacggcaatc ggtgttgcat agcgagccca gagctgacgc atcatctcca cccagccgcc
     2161 ttggccgccg tgatcgaccc tcacggcttc ggtgtcgacg tatgccatca agccgttccc
     2221 gccatgcagg tctggaggat agagggcgag ccggtggtcc aggaaacgat cgctggtggc
     2281 atagtggttc aagcctatga cgtcggccgg acagggtgac ttcgctacgg aggcgagccg
     2341 atccccgaat ccgaagcttg agatgcggtc gtagagaggg tgaccctccc ctacctggcc
     2401 tgccaggagg tcccaggtcg cgagccggcg aatgttctca tgttcgacct gctcaaggca
     2461 cgctgccgtg ccgaaggtga tcccgaaatc ctccgtctgg acgaggcggg cgccagggat
     2521 aaccgcgcgg atcgcgctca ttgcgagccg cgtcgcgtcg acctgattga gcagggcgcg
     2581 ccagcagtcg ccctcagaga cggcgtgcgg ataccagtga ccgtacaggg cgctgaaacg
     2641 tgcggtcgtc agaggctcgt taaccggggt ccagtcctgc acccaggggt accgccgcgc
     2701 ggcctccccc gcgaaccgcg ccagacccgg cgcaaaacca tcgtcgagaa ggttggtcca
     2761 gcgagggcct gagccgtgat ggatcaagcc gacgataggt cgtatcccga gcgccctcaa
     2821 ccgctccatg cggcggtcca tccaggacca gtcgaaacgg ccgggcttgg tttcgatccg
     2881 ctcccagacc atcgggtagc gcagggtcct tatgctcaac gccgcgatct gttcgagatc
     2941 gtcaatgcgg tcc
//
LOCUS       DASVTM010000150         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_386326,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000150 DASVTM010000000
VERSION     DASVTM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386326"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1157
                     /locus_tag="VF633_04240"
     CDS             183..1157
                     /locus_tag="VF633_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX8470300.1"
                     /translation="MKAAVLAAALPVASAQAWEPTKPIEIIVPFPPGGSSDQMARTIQ
                     GAIQKNNLVSQPIIIVNKPAAAGGEAMLDIQKSAGDPHKLITTSSGIYMTPLATKLPV
                     SWKNFTPVAMMAQDAFLLWVNASQPYKTAKDFVAAAKAANPPLKTGGNGSKREDHLIS
                     VTMEQAAGIKITYIPYQGGGPSSVQLAGGHIDANMNNPAEEISNWRSGAVKPLCVFSA
                     KPLAYTQKITADMAWADVPTCVSQGLDVNYQMLRGMFMPGKVTPEQQAFYVDLFRKVT
                     ETADWKTYVERNALVPDFRSGNDFVEFLTTDEQKHKDLMGKAGFIGTN"
     gene            1461..1958
                     /locus_tag="VF633_04245"
     CDS             1461..1958
                     /locus_tag="VF633_04245"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX8470301.1"
                     /translation="MADTTLTPPEPGLSRRVVEIIVALILMALAGMVLWDSYERGAGW
                     DGGPQNGFFPARVGWILLIAAIAAFVSAIRREDSVFVTYGQLRMVGSVFAPLFLYVLA
                     IEFVGIYVASALFMAVFMVMLGDFRWWQTLLAVIAVPVVCFWVFEIQFRLSLPKGPVE
                     TMLGF"
     gene            2156..>2814
                     /locus_tag="VF633_04250"
     CDS             2156..>2814
                     /locus_tag="VF633_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX8470302.1"
                     /translation="MFMVVGVLLGIVVGVLPGLGGPNGVAILLPLTFGMDPTSAIILL
                     SSIYWGALFGGAITSILFNIPGEAWSVATTFDGYPMAQQGRAGEALTAAFTASFIGAL
                     SGVILITFVAPLVAKFALRFGPAEFFGVFFLTFCSFIGMGKENKAKIVASLAIGFLLA
                     AVGLDTISGDLRLTFGSSELIKGFDFLVVVIGLFGVSEILLTIEEGLHFKGKKAAIDL
                     K"
BASE COUNT          460 a          957 c          880 g          517 t
ORIGIN      
        1 ttcaaaacga ttgtcgagct tgtccggtcc cttgaacctg tggaatttat catacaagct
       61 tgcgttgacg gtgaggcgtc cgtcccggcc ggttgccaac gacttccaaa gcctcgtacc
      121 tgtcgcgtga cgatggtgcc gaaaagagca cctttcagag gaagacgccc atgagacgct
      181 tcgtgaaggc ggccgtgctg gccgcggcgc tgccggtcgc gtcggcccag gcctgggaac
      241 cgacgaaacc catcgagatc atcgtaccgt tcccgcccgg cggctcatcc gaccagatgg
      301 cgcggacgat ccagggcgcg atccagaaga acaatctcgt ctcgcagccg atcatcatcg
      361 tgaacaagcc ggccgcggcc ggcggcgagg cgatgctcga catccagaaa tcggcgggcg
      421 acccgcacaa gctcatcacc acctcgagcg gcatctacat gaccccgctc gcgaccaagc
      481 tcccggtgag ctggaagaac ttcacgcccg tggcgatgat ggcccaggac gccttcctgc
      541 tctgggtgaa cgcaagccag ccctacaaga ccgccaagga cttcgtggcg gccgccaagg
      601 ccgccaaccc gcccctgaag accggaggca acggctcgaa gcgcgaggat cacctcatct
      661 ccgtgacgat ggagcaggcg gccggcatca agatcaccta catcccctat cagggcggcg
      721 ggccgtcctc ggtccagctc gcgggcgggc atatcgacgc caacatgaac aacccggccg
      781 aggagatctc caactggcgc tcgggcgcgg tgaagccgct gtgcgtgttc tccgcaaagc
      841 cgctggccta cacgcagaag atcaccgccg acatggcctg ggccgatgtc ccgacctgcg
      901 tctcgcaggg tctcgacgtc aattaccaga tgctgcgggg catgttcatg ccgggcaagg
      961 tcacgcccga gcagcaggcg ttctacgtcg acctgttccg gaaggtcacg gagaccgccg
     1021 actggaagac ctatgtcgag cgcaacgcgc tcgtgcccga cttccgctcg ggcaacgact
     1081 tcgtcgagtt cctcaccacc gacgagcaga agcacaagga cctgatgggc aaggccgggt
     1141 tcatcggcac gaactgaggc ggttcccggt gatggcttcc atgctggcct ccgcgtcctt
     1201 ccccggacgc ccggagcgcc agcggcgggt gatccggggt ccagctcggg acgtgccgca
     1261 tcagcgggtc gatctcgatg acgcaacgac gtcggacgct tcgcggcctc gcccgctggg
     1321 tcccggatcg ggttccgctt cgctccaccc gtccgggaaa aggcgggggg agacaccggg
     1381 gcggtccatc cgacttccca tgaaacctaa gccggcccga tgcgccgggc tcgcttcctc
     1441 tctgtccgga taagttgatc atggcagata ccactctcac gccccccgag cccggcctgt
     1501 cgcgccgggt ggtggagatc atcgtcgccc tcatcctgat ggcgctcgcg ggcatggtcc
     1561 tgtgggattc ctacgagcgc ggggccggct gggacggtgg tccccagaac gggttcttcc
     1621 ccgcccgcgt cggctggatc ctgctcatcg ccgcgatcgc cgccttcgtg agcgccatcc
     1681 ggcgcgagga cagcgtcttc gtcacctacg gccagctgcg catggtgggt tcggtcttcg
     1741 cgccgttgtt cctctacgtg ctggctatcg agttcgtcgg catctacgtc gcctcggcgc
     1801 tgttcatggc cgtgttcatg gtgatgctcg gcgacttccg ctggtggcag acgctgctcg
     1861 cggtgatcgc cgtgccggtg gtctgcttct gggtgttcga gatccagttc cgcctgtccc
     1921 tgcccaaggg tccggtcgag acgatgctcg gcttctaagc atcatttccg cgaagtggtc
     1981 cgcggttcgg cggagacaat gatgcggcat cgagaacacc caagcaggca atggcggggc
     2041 aacccacgaa tgcctgctta gaccaccgac cgagacgaaa ccgaggaacc ccgccatgga
     2101 cgatctccac cagctcatgt tgggcttcca ggtcgctctg tcctggacca acgtcatgtt
     2161 catggtcgtg ggcgtgctgc tcggcatcgt cgtcggcgtg ctgccggggc tcggcggccc
     2221 caacggcgtc gcgatcctgc tgccgctcac cttcggcatg gacccgacct cggcgatcat
     2281 cctcctgtcg tcgatctact ggggcgcgct gttcggcggc gccatcacgt cgatcctgtt
     2341 caacatcccc ggtgaggcgt ggtccgtcgc caccaccttc gatggctacc cgatggctca
     2401 gcagggccgg gcgggcgagg cgctcacggc cgccttcacc gcctcgttca tcggcgctct
     2461 ctcgggcgtg atcctcatca ccttcgtggc gccgctcgtg gcgaaattcg ccctgcgctt
     2521 cggcccggcc gagttcttcg gggtgttctt cctgacgttc tgctcgttca tcggcatggg
     2581 caaggagaac aaggccaaga tcgtcgcctc cctggcaatc ggcttcctgc tcgccgcggt
     2641 cggtctcgac accatctcgg gcgacctgcg cctcaccttc gggtcgagcg agctcatcaa
     2701 gggcttcgac ttcctcgtcg tggtgatcgg cctgttcggc gtgtccgaga tcctgctcac
     2761 catcgaggag ggcctgcact tcaagggcaa gaaggcggcg atcgacctca agat
//
LOCUS       DASVTM010000151         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_600447,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000151 DASVTM010000000
VERSION     DASVTM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600447"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1373)
                     /locus_tag="VF633_04255"
     CDS             complement(84..1373)
                     /locus_tag="VF633_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX8470303.1"
                     /translation="MFSLLNLQSLFGIVVIVGACWAISEDRKAFPWRLTLGAIAVQAL
                     LVLALFAIPGSQVVLHGITGAVDGLSVATAEGARFVFGFLVDGRQDAPYYLTNTDPRI
                     SAPFTFAFNVLPMILVISALSALLWHWKILKWVTHGFGFLFQKTMGLGGASALAVAAN
                     IFLGMIESPIVIRAYLDKLTRSEIFLMMVVGLATVAGSTMVAYAAILSPVLANAAGHV
                     LVASIVSAPAGVLLARIIIPEKPGEGGAVADYNTALKYDSSIDAISKGTADGLMVVMN
                     ISAVLIVFVALVALVNIMIGGIVIFDAPLTLQRALGWLFMPIAWLTGVQWSETGPAGL
                     LLGVKLTLTEFVAFIDLGKVSPAVMSERTRMIMTYALCGFANIGSVGITVSGLSVLMP
                     ERREEVLGLIWKALFAGFLSTLMTAAVVGAMPAVIFG"
     gene            1623..>1991
                     /locus_tag="VF633_04260"
     CDS             1623..>1991
                     /locus_tag="VF633_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470304.1"
                     /translation="MSSDSRGNRSGPVAGAGVGRHLLIVDDDDRIRELLKEYLAREGY
                     RTTGAAHAAAARRLMELIEFDLVVLDVMMPGESGLELTSWVRSKAQLSRTPVLLLTAR
                     GEASDRIDGLSRGADDYMSKP"
BASE COUNT          399 a          629 c          643 g          320 t
ORIGIN      
        1 cagcggaccc ggcgaggatt gggcgcgcga tgtgaggtgt agagcttcat cgacgtttcc
       61 tgatgccgcg tccgggtggc ttgttagccg aaaatgaccg ccggcatcgc gccgacaacc
      121 gccgccgtca tcagggtcga caggaagccc gcgaacaaag ccttccagat cagccccagc
      181 acctcttcgc gccgctcagg catcagcacc gacagacccg agaccgtgat cccgacggag
      241 ccgatgttag cgaaaccgca gagggcatag gtcatgatca tgcgggtgcg ttcgctcatc
      301 acggcgggcg aaaccttgcc cagatcaatg aaggccacga actcggtcag cgtcagtttg
      361 acgcccagca gcagtccggc aggaccagtc tctgaccact gaacgcccgt cagccaggcg
      421 atgggcatga acagccagcc cagagcccgt tgcagcgtca gcggggcatc gaatatgacg
      481 atgccgccga tcatgatgtt caccagcgcc accagcgcca cgaacacgat cagcacagcc
      541 gagatgttca tcaccaccat caggccatcg gccgtgccct tggagatggc gtcgatggac
      601 gagtcgtatt tcagcgcggt gttgtagtcg gccacagctc caccctcgcc cggcttttcg
      661 ggaatgatga tacgggccag cagcacccct gcaggcgccg agacgatcga ggcgaccaac
      721 acgtggcccg ctgcgttcgc aagcaccggc gacaggatgg cggcgtacgc gaccatggtg
      781 gacccggcga cggtcgctag cccgacgacc atcatcagga agatttcgga ccgggtcagc
      841 ttgtcgagat aggcgcggat gacgatcggg ctctcgatca tgccgaggaa aatgttggcc
      901 gcgaccgcca gtgccgaggc tccgcccaga cccatggtct tctggaacag gaagccgaag
      961 ccgtgagtga cccacttcag gatcttccag tgccacagca gcgccgacaa cgccgagatc
     1021 accagaatca tcggcagaac gttgaaggcg aaggtgaacg gcgccgagat gcgggggtca
     1081 gtgttggtca aatagtaggg cgcgtcctga cgtccatcga cgaggaagcc gaacacgaac
     1141 cgggctccct cggccgtcgc cacggacagg ccatcgactg cgccagtgat gccatgcagc
     1201 accacctgcg atcccgggat ggcgaacagg gccagcacca gcagcgcctg cacggcgatc
     1261 gctccgagcg ttagccgcca ggggaaggcc ttgcggtcct cggatatcgc ccagcaggcg
     1321 ccgacgatga ccacgatgcc gaacaggctc tggaggttca gcagactgaa catgaagcga
     1381 agatccccga cgtcttccac gctggtcagc gtcggaagct caatcctcgc ttgaacgcca
     1441 aggcgcggcg cactgcaagt cccccgaggt catgaatccg gggcggtgac ggtccgatgt
     1501 cagccttcag atgtgccaat cagccttcct ccgtcgaaac atggcctctg tcgcctgtcc
     1561 gtcgtataga ttcgctgtct tgccggacgc ggcgagcgac gggctgggta tggaattcga
     1621 gcatgagtag tgattcacgg ggcaaccggt cgggtccggt cgccggagcc ggcgtggggc
     1681 gtcatcttct gatcgtcgat gacgacgacc ggattcgcga actgctgaaa gaatatctgg
     1741 ctcgcgaggg ctatcgcacc accggcgcgg cgcacgctgc ggcggcgcgt cggctgatgg
     1801 agttgatcga gttcgacctc gtcgtgcttg acgtcatgat gccgggcgag agcgggctgg
     1861 agctgaccag ttgggtgcgt tccaaggcgc agttgtcgcg gacgcccgtc ctgctgctga
     1921 ccgcccgcgg cgaggcctcg gaccggatcg acggcctgtc gcgcggcgcg gacgactata
     1981 tgtccaagcc g
//
LOCUS       DASVTM010000152         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_120434,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000152 DASVTM010000000
VERSION     DASVTM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120434"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1430
                     /locus_tag="VF633_04265"
     CDS             141..1430
                     /locus_tag="VF633_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8470305.1"
                     /translation="MFVRFFAIPLWQRTAAGFILGIIAGLILRESAVTWLQPIGDLYL
                     NLIRMVVAPLVLFTIASSIAKLGEGAGAVRLGVKTIMWFAITSFLAVLVGFAFAHLIN
                     PGVGLANLPLGEVKERVIPTPLEVLLGVVPTNPFAALSEGKVLQIIFFSALVGVALVA
                     LGDRAANARRLVDEGAAIIFRITRWVIQLTPFGVFGLIGAIVGGYGWEALLPLIKFIV
                     AIYAACLFHIVFVYGALLKLHGLKVTSFFRGAFAAQQTAFATSSSLGTLPITLRQTVE
                     RLGVPQAYAAFAVPLGANVKMDGCGAIYPAIASIFIAQYFKIDLSLTQYILIGLTAVL
                     GSLGTAGVPGTSIVMLTLTLSTAGLPLEGIGYIVAIDRIIDMMRTATNVTGQMLVPVL
                     VAKEEGVLNRDIYDGHVAWLPGDPETDAPDLVKAGGI"
     gene            1508..1582
                     /locus_tag="VF633_04270"
     tRNA            1508..1582
                     /locus_tag="VF633_04270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Val,seq:cac)
     gene            1868..3034
                     /locus_tag="VF633_04275"
     CDS             1868..3034
                     /locus_tag="VF633_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470306.1"
                     /translation="MKRIIIIAVAATLASAGAAWAGGGNGGNGGGGHPGGGGGCGSSC
                     GGGGGHGGGNWGGNFNNNVNVNVNANANANANANSNSNSSYGRSVLNARSYSGYGSIR
                     SGGGGGGYAYVDDGYAPAGPGYGGGVQLSADCGCIAGPSQPFGYSVSGFGRNLARTQG
                     SIAYSQQSYSQGGYAQSSYSQGSRYSSTSSYGGASACYSCEAPAPVPSCGPYPGGYGG
                     GYGGDYGACGPAPAPQPYPGPDCDCDAPVYAQPFYAPPIGPRPVAVRSQGVRIVSPPV
                     NVPPQLVYVQGPPVWVDAPPVNVAPAQIYLEAPNVRVRPSEVTVAPPEIHFTPAQPHP
                     EDDCCNVASGAAIDAATASQYQESSYSSSAQSGSAAYVQGTPVQSHNTGPEYFQ"
     gene            complement(3128..3601)
                     /locus_tag="VF633_04280"
     CDS             complement(3128..3601)
                     /locus_tag="VF633_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470307.1"
                     /translation="MNRYLTTTALVLALAAGAGLSSCDKAGKAAAPAVPAATALTAPA
                     TEAAPETESAELPPLIPDIAAAHNWVRADQPPEERAVACAALTALQLQAIDAGAPGDK
                     AAIKAAYDAWNAELIKQTESKDSADQYFASTFAVLDDTPPAPLKAAADDCAAHKP"
     gene            complement(3601..>4351)
                     /locus_tag="VF633_04285"
     CDS             complement(3601..>4351)
                     /locus_tag="VF633_04285"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8470308.1"
                     /translation="IMVGVSDYGDDDMNLPYTDEDASKLAETLRREGVLNPSSVTLTN
                     AQATVAGVRQAFQRVAQQAGPNDMFLFFFSGHGDQTEGNVSDVSAFEPDGRTESIVLR
                     DGEISDFEMARLFGTLRTRLSLLVLDSCYSGGFARNVVDRPGVMGLFSSEEDLTSAVA
                     DKFEAGGYLAHFLRTGLSGDADGDGDAMVTAGELTTYLRRQFTNQVENVEAETSEGQR
                     NYQNLVIDRGGVQIDDVILRLGTTNQARGVH"
BASE COUNT          660 a         1505 c         1356 g          830 t
ORIGIN      
        1 ggcccggggt ttccaatgtc tcctttttgg cgtttttttc taaaccggag actctgatcc
       61 cttcgccacc cacccggttg cggtttggtg tcgcagtccg cacattcgcg aaccatcgct
      121 tccccgaaag ccgttcgctc atgttcgtcc gcttcttcgc catcccgctg tggcagcgca
      181 ccgccgccgg gttcatcctc ggcatcatcg ccggcctgat cctgcgtgag agcgccgtca
      241 cttggctgca gccgatcggc gatctctatc tgaacctgat ccgcatggtg gtcgcgccgc
      301 tggtgctgtt caccatcgcc tcgtccatcg ccaagctggg cgaaggcgcc ggcgcggtgc
      361 ggctgggcgt caagaccatc atgtggttcg cgatcacgtc gtttctggcg gtgctggtcg
      421 gcttcgcctt cgcccacctg atcaacccgg gcgtcggcct cgccaacctg ccgctgggcg
      481 aggtcaagga acgggtcatc ccgaccccgc tcgaggtttt gctgggcgtc gtccccacca
      541 acccgttcgc cgcgctcagc gaaggcaagg tgctgcagat catcttcttc tcggcgctgg
      601 tcggcgtggc tctggtcgcg ctgggcgatc gcgccgccaa cgcccgtcgt ctggtcgacg
      661 aaggcgcggc catcatcttc cgcatcaccc gctgggtcat tcagctgacc ccgttcggcg
      721 tgttcggcct gatcggcgcg atcgtcggcg gctatggctg ggaggcgctg ctgccgctga
      781 tcaagttcat cgtcgccatc tacgccgcct gccttttcca catcgtcttc gtttatggcg
      841 ccctgctgaa gctgcacggg ctcaaggtga cgtccttctt ccggggcgcg ttcgccgccc
      901 aacagacggc cttcgccacc tcctcgtcgc tcggcaccct gcccatcacc ctgcgccaga
      961 cggtcgagcg cctcggcgtg ccgcaggcct atgccgcctt cgccgtaccg ctcggggcca
     1021 acgtcaagat ggatggctgt ggcgccatct atccggccat cgcctcgatt ttcatcgccc
     1081 agtatttcaa gatcgacctg tcgctgaccc agtatatcct gatcggcttg accgccgtcc
     1141 tcggctctct cggcaccgcg ggcgtcccgg gcaccagcat cgtcatgctg acgctgacgc
     1201 tctccaccgc cggtctgccg ctggaaggca tcggctatat cgtcgccatc gaccggatca
     1261 tcgacatgat gcgcacggcc accaacgtca caggccagat gcttgtgcca gttctggtcg
     1321 ccaaggaaga gggtgtgctg aatcgggaca tctacgacgg ccacgtggca tggctcccgg
     1381 gcgatcccga gacggacgct cccgatttgg tcaaggcggg cggcatctga cgcttgctct
     1441 ttagccggcg tcgagctaca tcgccgcctc cccggtcggg ccctgaacag gcccgacacg
     1501 ccggtccgga tgcttagctc agcgggagag cacctcgttc acaccgaggg ggtcgcaggt
     1561 tcaatccctg cagcatccac catctccctt ctcccgaatt agtccgcaaa accctgcagc
     1621 atctaggttt gaaccctcgt cgggtattcg ttcgtgggga tgccaatcgt ggttaagtct
     1681 cgccgcctgc ggacacagca tccaagcttc accgcagaga ccgcgaactc cagttcatca
     1741 gcaataacag ccgatcggct cgcctgacgc gcgcattctt atggttactt tttgcgtacg
     1801 gggtgaaagc ggctttcccg gcgcatctct tgctctagct ccagtcaaat acattggagc
     1861 gatcaccgtg aaacggatca tcatcatcgc agtcgcagcg accctcgcct ccgcaggcgc
     1921 ggcctgggct ggaggcggca acggcggaaa cggtggcggt ggccatcccg gtggcggagg
     1981 cggctgcggc tcgtcctgcg gcggcggcgg cggtcacggt ggcggcaatt ggggcggcaa
     2041 cttcaacaac aacgtcaatg tgaacgtcaa cgcaaacgcc aatgcgaacg cgaacgccaa
     2101 ctcgaacagc aacagcagct acgggcgcag cgtcctaaac gcccgttcct acagcggcta
     2161 cggctcgatc cgtagtggcg gcggcggtgg cggttacgcc tatgtcgacg acggttatgc
     2221 gccggccggt cccggctatg gcggcggcgt ccagctttcg gccgattgcg gctgcatcgc
     2281 cggtcccagc cagcccttcg gctattcggt ctccggcttc ggtcgcaacc tcgctcgcac
     2341 ccagggttcg atcgcctatt cgcaacagtc ctactcgcag gggggatatg cgcagagcag
     2401 ctactcgcag ggttcccgct attcgagcac gtccagctac ggcggcgctt cggcctgcta
     2461 ctcctgcgaa gcgccggcgc ccgttccctc gtgcggccct tatcccggcg gctacggtgg
     2521 cggatatggt ggcgattacg gcgcctgcgg tccggctcca gcgccccagc cgtatcccgg
     2581 cccggactgc gattgcgacg cccccgtcta cgcccagccc ttctacgctc cgcccatcgg
     2641 tccgcgtccc gtggcagtgc gttcgcaggg cgtccggatc gtctcgccgc cggtgaatgt
     2701 cccgccgcag ctcgtctacg tgcaggggcc gccggtctgg gtcgatgcac cgccggtcaa
     2761 cgtcgcgcct gcccagatct atctggaagc tccgaacgtc cgcgtccgtc cgtcggaagt
     2821 caccgtggct ccgccggaaa tccacttcac cccggctcag ccgcatccgg aagacgactg
     2881 ctgcaacgtc gcctcgggcg ccgcgatcga tgcagctacg gcgtcgcagt accaggagtc
     2941 gagctattcg tcctcggctc aatccggttc ggccgcctac gtccagggca cgccggtcca
     3001 gagccacaac accggtcctg aatacttcca gtaagcgggc ccggggcctc actcgcggcc
     3061 ctgagctcaa gaaaacgccc ggcccatgga ttacgggccg ggcgttgcct tgtctgggcg
     3121 ggtcattcta cggcttgtgg gcggcgcagt cgtcggcggc ggccttgagc ggcgcgggtg
     3181 gcgtatcgtc cagaaccgcg aaggtgctgg cgaaatactg gtcggcgctg tccttgctct
     3241 ctgtctgctt gatcagctcg gcgttccagg cgtcgtaggc ggccttgatc gccgccttgt
     3301 cgccgggcgc acccgcgtcg atggcctgta gctgcagagc ggtcagggcg gcgcaggcga
     3361 cggcgcgctc ctcgggcggc tgatcggccc tgacccagtt gtgagccgca gcgatgtcgg
     3421 ggatcagagg cggcagctcg gcggattcgg tttcaggagc ggcctcagtc gcaggagcgg
     3481 tgagtgccgt cgcagcaggc acagccggag ccgccgcctt cccggccttg tcgcagctcg
     3541 aaagaccggc tccggccgcc agagccagca ccagcgcagt cgtcgtcaga taccggttca
     3601 tcagtggacc ccacgagctt gattggtcgt accgagtctg aggatgacgt cgtcgatctg
     3661 gacgccgccc cggtcgatga cgaggttctg gtagttgcgt tgcccctctg acgtctcggc
     3721 ctcgacgttc tcgacctggt tcgtgaactg gcggcgcaga taggtggtca gctcgcctgc
     3781 cgtgaccatc gcatctccgt caccgtcggc atcgcccgac agcccggtgc gcaggaaatg
     3841 cgcgagataa ccaccggcct cgaacttgtc ggccacggcg ctggtcaggt cttcctcgga
     3901 gctgaacagg cccatgacgc ccggccgatc gacgacgttc ctcgcaaagc cgcccgagta
     3961 acagctgtcg agcaccagca ggctcagccg cgtccgcagc gtgccgaaca accgcgccat
     4021 ctcgaagtcc gagatctccc cgtcgcgcag gacgatggac tccgtccggc cgtcgggctc
     4081 gaaggcgctg acgtcggaga cgttgccctc ggtctgatcc ccatgacccg agaagaagaa
     4141 caggaacatg tcgttcggcc cggcctgctg ggccacgcgc tggaaggcct gacgcacgcc
     4201 tgcgaccgtg gcctgggcgt tggtcagggt gacgctggac gggttcagca caccctcgcg
     4261 acgcagggtc tcggccagtt tcgacgcgtc ctcgtcggtg tagggcaggt tcatgtcgtc
     4321 gtcgccgtag tcggaaaccc cgaccatgat g
//
LOCUS       DASVTM010000153         5736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_333149,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000153 DASVTM010000000
VERSION     DASVTM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333149"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..435)
                     /locus_tag="VF633_04290"
     CDS             complement(22..435)
                     /locus_tag="VF633_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX8470309.1"
                     /translation="MTDAPTTGWADQLAPSLDDFARLAEQAFADLPEEFRAAAGEVVF
                     RVDDFADEETLAHFGMEDPFELSGLYHGVDIGRRDTLGPAVGPSRIFLYRRPILDEWC
                     ERGDVGLAEIITHVLIHEIGHHFGLSDADIDAAER"
     gene            579..2975
                     /locus_tag="VF633_04295"
     CDS             579..2975
                     /locus_tag="VF633_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470310.1"
                     /translation="MAEAEIAFVASAGAAGLALAVSAWAWRLRQRLAVREASVEAHEA
                     ERLAALSEREGALTAFDDIRIGLTSDGGVTSLLGSTAGVIAARAALCPDDEGADDAVV
                     LATAIHAAWPDAIRALVEQGEACDLRIDTPSSDADPDGAEDRATQGRGSGQSTGWSVE
                     GRLTGGVAWLRLYPTPHSQPGIEAGLGLMTDASPAPTWVVDPQGKLIWANRAWLAEVG
                     ATSLDEAREKGLSFDRGADTLLIEAGRIGTRQEGFRWTTGGGRRRAWRIVADPLARTG
                     AGTGAVLAFAVDMTEAEETRDTLRRHVEAHDETLNHLADAVAIFGPAKRLAFHNTAFQ
                     TLFNIDPAWLDERPTHAELLDRLRQKRQLPEVVDYAGWKAGELDFYGAAAAAPDDSWS
                     LPDGRTLRVVRQPHPLGGILLLFSDITDELKLRSRFNAQIQVQTATLDKLNDAVAVFG
                     SDGRLRLHNEAFETFWSLSNEQLVEASDFDAVAELAKTIMPDPALWLGLKARVADPDP
                     ESRVPISGEGRTTDGRIAAWQTRPLPDGATLVAFSDVTARRELEQALAAKAAALEESQ
                     ALKREFVGSVSYELRTPLTTIVGYSELLETLGGLNERNKQYAGAIRIAASQLARSIDD
                     VLDMAQIDAGEMELALGDVRIEDLFAAAAERFRPRVEGRGGRLIVQTMAASRAIRGDG
                     RRIAQALDHLLENATRGVSEGGVVTLRADGNSSEVRIRVEDTGRGIPYHLQAHVFDRF
                     VRRDRGGPGVGLALVKALVELHGGWAEVESEPGKGAAFILHLPIDAHGDAAAPELQLG
                     "
     gene            complement(2983..3612)
                     /locus_tag="VF633_04300"
     CDS             complement(2983..3612)
                     /locus_tag="VF633_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX8470311.1"
                     /translation="MPSTPLLTRAAILAAVGAAVVLGGATVKASAQSTAPAPTPALAS
                     GGWLVDAARNRDTEAAQVFVSHGANVDEGRASDGTPLIVAARNGDLVMARFLVERGAG
                     VDRAMKGDGNPLIAAAANGRLDMADYLISLGADVNAVVSDDETPLINAAREGHLEMVQ
                     RLVKAGANVNLAVPVTLASGATEIRSPLNQARHRQAAVVAWLAAQGARR"
     gene            3727..4572
                     /locus_tag="VF633_04305"
     CDS             3727..4572
                     /locus_tag="VF633_04305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8470312.1"
                     /translation="MTKLLKTAIIYDFDGTLARGNMQEVSFIPSIGVDIGAFWAEAEA
                     LTEAADADGILMYMQLMLRHARDNGAPITRETLREHGREVALFEGLKDLSWFDRMNAF
                     GAQYGLDIEHYIISAGLEEMIEGTPIRSALTHVFASHYVYDENGEAAWPAVGVNYTTK
                     TQYLFRINKGVNNHWEHERINHFIPDEERSIPFERMIFLGDGDTDVPTMKMMHTKGGF
                     SIAVYDPRSNEKDQKKVYSLISEDRVNFVAAADYREGSALDLIVKGLVGRIAINSGTM
                     PADAR"
     gene            complement(4594..5496)
                     /locus_tag="VF633_04310"
     CDS             complement(4594..5496)
                     /locus_tag="VF633_04310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8470313.1"
                     /translation="MPVLTRPEGLSAEDLKFSRRTLGVSAVGGLFFSQYAVAALAQTA
                     QPIVTDANGLTTADVTYPAPDGFALPAYVARPEGDGPFPVVIVVSEIFGVHAYIQDIC
                     RRLARQGYAAIAPAFFVRGEDPAPLSDMSRIREIVGAARYEQVMGDVSATLDWTSQQL
                     WADSDKVGITGYCWGGKVVWQSCARFPAIDQGVAWYGQLAPSPTATPEQASAGSPWPV
                     DLARDLKCPVLGLYGELDQGIPQTAVEAMRAALRTAGQTTSEIVVYPGAQHGFHADYR
                     ASYDATSAADGWSKLLKVFEAKLK"
     gene            complement(5568..>5736)
                     /locus_tag="VF633_04315"
     CDS             complement(5568..>5736)
                     /locus_tag="VF633_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX8470314.1"
                     /translation="AIGGKTTTVKLASGDICALTGAARLARHGIDRTLEGSSRLVPGG
                     GRLNLTLRRAM"
BASE COUNT          970 a         1827 c         2019 g          920 t
ORIGIN      
        1 ggccccaggg cgaaagacgc gctagcgctc cgcggcgtcg atgtcggcgt cggagaggcc
       61 gaagtggtgg ccgatctcgt ggatcaggac gtgggtgatg atctcggcca gaccgacgtc
      121 gccgcgctcg caccactcgt cgaggatcgg gcggcgatag aggaaaatcc ttgacggccc
      181 cactgcgggt ccaagcgtgt cacgacgacc gatgtcgacg ccgtggtaga gaccgctgag
      241 ttcgaacgga tcctccatgc cgaagtgagc cagcgtctcc tcgtcagcga agtcgtcgac
      301 gcggaacaca acctcccccg ccgccgcgcg gaactcttcg ggcaagtcgg cgaaggcctg
      361 ttccgccagc cgggcgaagt cgtcgagtga tggggctagc tggtcggccc agccggtcgt
      421 cggagcatcg gtcatgaccc catatcgagc cggcgaggcc ccttgcgcaa tgcgacagcg
      481 aggcgtcaaa ccgcgctgct tgaagctgat ccagcgcaaa aggcgtcagc ggccgaaaat
      541 cggttatggt gaatcaacga atcggcgggt gaacagtcat ggcggaagcc gaaatcgcct
      601 ttgtggcgtc ggcaggcgcg gcggggctgg ccttggcggt cagcgcctgg gcctggcggc
      661 tgcgtcagcg gctggcggtg cgcgaggcct cggttgaagc gcatgaggcc gaacggctgg
      721 cagccctgtc ggagcgcgaa ggtgcgctta cggccttcga tgacattcgc atcggtctga
      781 cctcggacgg aggcgtgacc agtctgttgg gctccaccgc aggcgtcatc gccgcccggg
      841 ccgccctgtg ccccgacgat gaaggcgctg acgacgccgt cgtgctggcc accgctatcc
      901 acgctgcctg gcctgacgcg atccgcgcgt tggtcgaaca gggcgaggcc tgcgacctga
      961 ggatcgacac accgtccagt gacgcagatc cagacggcgc ggaggaccgc gcaactcaag
     1021 gccgaggcag cggccaaagt accggctgga gcgtcgaggg gcgactgacg ggcggagtgg
     1081 cgtggctgag gctctacccg acgccgcatt cgcaaccggg catcgaagcc gggctgggtc
     1141 tgatgaccga tgcctcccct gcgccgacgt gggtggttga tccgcagggc aaactgatct
     1201 gggccaatcg ggcctggctg gccgaggtcg gggcgacgag cctcgacgag gcccgcgaga
     1261 agggattgag cttcgaccgg ggcgccgaca cccttctcat tgaggccggg cggataggca
     1321 cgcgtcagga aggcttccgc tggaccaccg gcggcggccg tcgtcgcgcc tggcggatcg
     1381 tcgccgatcc tctggcgcgg acgggcgccg ggacgggggc ggtgctggcc ttcgcggtcg
     1441 acatgaccga ggccgaggaa acccgtgaca ccctgcgccg tcacgtcgag gctcatgacg
     1501 agaccctgaa ccacctcgcc gacgcggtcg ccatcttcgg gccggcgaag cggctggcct
     1561 tccacaacac cgccttccag acgctgttca atatcgatcc ggcgtggctg gacgaacggc
     1621 cgacccacgc cgagttgctg gatcggttgc gccagaagcg ccagttgccc gaggtcgtgg
     1681 attatgcggg gtggaaggcc ggggaactgg acttctacgg cgccgcagcg gctgcgcctg
     1741 atgatagctg gtccctgccc gatggccgaa ctctcagggt cgtgcgccag ccgcacccgc
     1801 tgggcggcat cctgctgctg ttctcggata tcactgacga gctcaaactg cgcagccggt
     1861 tcaacgccca gatccaggtc cagaccgcga cgctcgacaa gctgaacgac gccgtcgccg
     1921 tgttcggctc ggacggaagg ttgcgactgc acaacgaggc gttcgagacc ttctggagcc
     1981 tgtcgaacga gcaactcgtc gaggcgtcgg atttcgacgc cgttgccgag ctggccaaga
     2041 cgattatgcc ggatccggcc ctgtggcttg ggctgaaggc gcgggtggca gatcccgatc
     2101 ccgaaagccg tgtgccgatc tcgggcgaag gacggacaac ggacgggcgc atcgccgcct
     2161 ggcagacccg ccctctgcct gacggcgcga cgctggtcgc cttctccgac gtaaccgctc
     2221 gccgggagtt ggagcaggcc ctggccgcca aggctgcggc tctcgaagag agtcaggcgc
     2281 tcaagcgcga gttcgtcggc tcggtcagct acgaactgcg cacgccgctg accaccatcg
     2341 tcggctattc ggaattgctc gagacgttgg gcgggctgaa cgagcggaac aagcagtatg
     2401 cgggcgccat ccgtatcgcc gccagccagt tggcgcgttc gatcgacgac gtgctcgaca
     2461 tggcccagat cgacgccggc gagatggagc tggcgctggg cgacgtccgg atagaggatc
     2521 tgttcgccgc cgccgccgag cggttccgcc cgcgcgtcga gggacgtggc ggtcggttga
     2581 tcgtgcaaac catggccgcc tctcgcgcca ttcgcggcga cggtcgccgt atcgctcagg
     2641 ccttggatca tctgctcgag aacgcgacgc gcggcgtgtc cgagggtggc gtggtgaccc
     2701 tgcgcgcgga tggcaatagc tccgaggtcc gcatccgggt cgaggacacc ggccggggta
     2761 ttccctatca tctgcaagcg catgtgttcg accgcttcgt gcgtcgcgac cggggcggac
     2821 cgggtgtcgg gctggccctg gtcaaggcgc tggtcgagtt gcatggcggc tgggccgagg
     2881 tcgagagcga gcccggcaag ggcgcggcgt tcatactgca tttgcccatc gacgcacacg
     2941 gcgacgcagc cgcgcccgag ttgcaactcg gttaggcggc ccttagcgcc gcgcgccttg
     3001 ggccgcgagc caggcgacga cggcggcctg gcgatgacgc gcctggttga gcggggaccg
     3061 gatttccgtg gcgccggaag ccagcgtcac gggtacggcc agattgacgt tcgctccggc
     3121 cttcacgagg cgttgcacca tctcgaggtg accttcccgt gccgcgttga tcagcggggt
     3181 ttcatcatcg gagacaaccg cgttcacgtc agctccgagc gagatgagat agtccgccat
     3241 atcgaggcgg ccgttggcgg cggcggcgat cagcgggttg ccgtcaccct tcatcgcgcg
     3301 gtcgacgccc gcgccgcgct cgaccagaaa ccgggccatc acaaggtcgc cgttgcgagc
     3361 cgcgacgatg aggggcgttc cgtcgcttgc gcgtccttca tcgacattgg cgccgtgcga
     3421 gacgaagacc tgcgccgcct cagtgtcgcg gttgcgcgcg gcatcaacca gccagccgcc
     3481 cgaagcgagg gccggggtgg gcgccggagc agtcgattgg gccgatgcct tgacggtcgc
     3541 cccgcccaga acgacggcgg cgccgacagc ggcaaggatg gcggcgcgcg tcaggagggg
     3601 cgttgaaggc atggtcggct ccttgaggtg gccaactgta ccagccctcc gccgggtgcc
     3661 tacttaaggt ttggtcatgt gggcgcaggg ctggcgcggg agtgaggctg ggaataagga
     3721 cgcagcatga cgaaacttct gaagaccgcc atcatctatg atttcgacgg cacactggcg
     3781 cgtgggaaca tgcaggaggt gtcgttcatc ccgtcgatcg gtgtcgatat cggcgccttc
     3841 tgggccgagg cggaagcgct gaccgaggcg gcggatgccg acggcatcct gatgtatatg
     3901 cagttgatgc tgcgccacgc ccgtgacaac ggcgcgccga tcactcgcga aaccctgcgt
     3961 gagcacggtc gcgaggtggc gctgttcgag ggcctgaagg acctgagctg gttcgaccgg
     4021 atgaacgcgt tcggggcgca gtacggtctg gatatcgagc actacatcat ctcggccggg
     4081 ctggaggaga tgatcgaggg cacgccgatc cggtccgccc tgacccacgt cttcgcctcg
     4141 cactatgtct atgacgagaa cggcgaggcg gcctggcctg cggtcggcgt caactacacg
     4201 acaaagaccc agtacctgtt tcgcatcaac aagggcgtga acaaccattg ggaacatgag
     4261 cggatcaacc acttcatccc ggacgaggag cggtcgatcc cgttcgagcg gatgatcttc
     4321 ctcggcgacg gggacaccga cgttccgacc atgaagatga tgcacaccaa gggcggcttc
     4381 tcgatcgccg tctatgaccc gcgctcgaac gagaaggacc agaagaaggt ctattcgctg
     4441 atctcggagg accgggtgaa tttcgtcgcc gccgccgact accgcgaggg ttcggcgctg
     4501 gacctgatcg tgaaggggct ggtcgggcgg atcgccatca actcgggcac catgccggcc
     4561 gacgcgcgct gaggcacgcc ggccatttgg cctctatttg agtttggcct cgaacacctt
     4621 gagcagcttg ctccagccgt ccgccgccga ggtggcgtca tagctggcgc gataatcggc
     4681 gtggaaacca tgctgggcgc cgggatagac aacgatctcg ctggttgtct ggcccgccgt
     4741 gcgcagggcc gcgcgcatcg cctccacggc ggtctgcgga atgccctgat caagctcgcc
     4801 gtacagaccc agcaccgggc atttcagatc cctggccaga tcaacgggcc agggcgaacc
     4861 ggccgaagcc tgttccggcg tggcggtcgg cgagggcgca agctggccgt accaggcgac
     4921 gccctgatcg atggcgggga agcgggcgca cgactgccaa accaccttgc cgccccagca
     4981 gtagccggtg atgccgacct tgtctgagtc agcccacagt tgctggctgg tccagtcgag
     5041 cgtcgccgat acgtcgccca tgacctgttc gtatctggcg gcgccgacga tctcgcggat
     5101 gcggctcatg tccgacagcg gcgccgggtc ctcgccgcgc acgaagaagg cgggggcaat
     5161 ggcggcatag ccctgcctgg ccagacgacg gcagatgtcc tgaatatagg cgtgcacgcc
     5221 gaagatctcg gacaccacga tcaccacggg gaagggaccg tcgccttcag gacgcgcgac
     5281 ataggccggc agggcgaagc cgtcgggcgc cggataggtc acgtcggcgg tggtcaggcc
     5341 gttcgcgtcg gttacgatgg gttgagcagt ttgggcgaga gcagcaacgg cgtactggct
     5401 gaagaacaga cctccgactg cactgacgcc aagcgtccgg cgcgagaact tcaggtcttc
     5461 ggcgctcagg ccttcggggc gggtcaggac gggcatgcgg ggctccggtt ggccaactgg
     5521 gaatgctggc ccgaccttga tcccgacgaa gacggtcgtc gagtccctta catcgcgcgg
     5581 cgcagcgtca gattgaggcg tccgccgccg gggaccaggc gcgacgatcc ctccagcgtc
     5641 cggtcgatcc catgccgggc cagtctcgcg gcgccggtca gggcgcagat gtcgccagac
     5701 gccagtttca ccgtggtggt ctttccgccg atcgcc
//
LOCUS       DASVTM010000154         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_440277,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000154 DASVTM010000000
VERSION     DASVTM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440277"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VF633_04320"
     CDS             complement(<1..723)
                     /locus_tag="VF633_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8470315.1"
                     /translation="MSTPFTHDIIIAGAGMAGATFGLAAAQGGLKVVMIDPQPFSTQL
                     EPAFDGRSTAIAYSTFRMLDTLGVGEALRPHACRMDHILVTDGHRPGAASHRPLPAFL
                     RFDASEIGDQNGDEPLGYMVENRQIRTALAQAVTAAGIEVRAPASVADVTRDGVSATV
                     TLKDGDVLTAPLVVGAEGRGSVVRRKAGIGTVGWGYGQSGVVATVRLGLPHGGVAHEY
                     FLPGGPFAILPLTDQRASLVWTE"
     gene            878..>2283
                     /locus_tag="VF633_04325"
     CDS             878..>2283
                     /locus_tag="VF633_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX8470316.1"
                     /translation="MSTALALSRHAWMSARVLWDAPFVVRFRGVLQALLATLLLVALI
                     SWNPADPSLNAASSTDATNWLGGNGALFADLSMQSVGLGAWPMTVLLVAFGLATAIGE
                     AIQQRLKPTPLKALSASAGVLLLSGALSALAAPAAWPLAVGIGGLWGDGLTGLFASGL
                     HALKIPGARAIVGVLFAGLGIWACAYAVGVRITDFAEAVGWASGLRRQAPPAPVPAPR
                     RTRTPKPKPMTEAAGLQSGAFAEVSTRAPILAEPEDAPTYDDADDGPPWEEPTAMAPT
                     SLAAAVEPRVAAPKTPKPAKNTGDPDQTMFEFNRPIGDFELPPLGMLAKPQPRVGMVD
                     EDSLKQNAKMLEGVLAEFGVKGVIDQIRPGPVVTLYELVPAAGVKHGRVVALSDDIAR
                     SMSARACRISVVQGRNAIGIELPNAKRETVYLRDLLASAEYAKPAHILPLALGESIGG
                     EPYVADLARMPHLLIAGTT"
BASE COUNT          350 a          844 c          736 g          353 t
ORIGIN      
        1 ctcggtccac acaaggctgg cgcgctgatc ggtcaggggc aggatggcga agggaccacc
       61 gggcaggaaa tattcgtgag ccacgccgcc gtggggaagc ccgagccgga ccgtcgcgac
      121 cacgccgctc tgaccatagc cccagccgac ggtgccgatc ccggccttgc gacgcaccac
      181 cgacccccga ccctcggccc cgaccacgag gggggctgtg aggacgtcac cgtccttcaa
      241 ggtcaccgtc gccgagacgc cgtccctcgt gacatcggcg acggaggcgg gcgcccggac
      301 ctcgatcccg gcagccgtaa cggcctgcgc cagagcggta cgaatctgcc ggttctcgac
      361 catatagccg agcggctcgt caccgttctg gtcgccgatt tccgaggcgt cgaagcgcag
      421 gaaggcgggc aggggccgat gcgacgccgc gccgggacga tgaccgtcgg tcaccagaat
      481 atggtccatc cgacatgcgt gcggcctcag cgcttcgccc acacccagcg tatccagcat
      541 gcggaacgtc gaataagcga tcgccgtcga acgaccgtcg aaggccggct caagctgggt
      601 cgaaaagggc tggggatcaa tcatcaccac cttcaacccg ccctgcgccg cagcgagtcc
      661 gaaggtcgct ccggccatgc ctgcgccggc gatgatgatg tcgtgggtga acggcgtgct
      721 cattcttcgc ttgtcgcgcc gtctcgcgtt cgcgtccaga ggacaagagg tccgcaccct
      781 catatccgac ctgccgaggc gctctggtaa acagcccctt aaccctgtcg gtgcgatgat
      841 cgggacgtac gcccgtcgcc gcaaggtctc gcccctcatg tccaccgccc tcgccctcag
      901 ccgccacgcc tggatgagcg cccgcgtcct gtgggatgcg cccttcgtgg tgcgttttcg
      961 gggcgtgctt caggccctgc tcgcgaccct gttgctggtc gccctgattt cgtggaaccc
     1021 ggccgatccc agcctgaacg cagcctcctc gacggacgcg accaactggc tggggggcaa
     1081 cggcgccctg ttcgccgatc tgtcgatgca gtcggtcggc ctcggcgcct ggccgatgac
     1141 ggtgctgttg gtggccttcg gtctggcgac cgccatcggc gaggcgatcc agcaacgcct
     1201 caagcccacg ccgttgaaag ccttgtcggc cagcgccggc gtcctgctgc tgtccggtgc
     1261 actgtcggcc ctcgccgcgc ccgctgcatg gcctctggcc gtcggcatag gcggcctgtg
     1321 gggtgacggt ctgaccggcc tgttcgccag cggcctgcac gccttgaaga tccccggagc
     1381 gcgggccatc gtcggcgtgc tgttcgccgg actgggtatc tgggcctgcg cctatgcggt
     1441 cggcgtccgc atcaccgact tcgccgaggc cgtgggctgg gcctctggtc tccgtcgtca
     1501 agcgccgccc gcgcccgtcc ccgcgccccg ccgcacccgg acgccgaagc ctaaaccgat
     1561 gaccgaagcc gccggcctgc agtccggcgc tttcgccgag gtctcgactc gcgcgcccat
     1621 cttggccgag cccgaggacg ccccgaccta cgatgacgcc gacgacggcc cgccgtggga
     1681 agagccgacg gcgatggcgc cgacctcgct ggccgccgcc gtcgagcccc gcgtcgccgc
     1741 acccaagacg cccaagccgg cgaagaacac cggcgatcca gaccagacca tgttcgagtt
     1801 caaccgcccc atcggcgatt tcgaactgcc gccgctgggc atgctggcca agccgcaacc
     1861 gcgcgtcggc atggtcgacg aggactccct caaacagaac gccaagatgc tcgaaggcgt
     1921 gctggctgag ttcggggtca agggcgtcat tgaccagatc cgcccgggcc ccgtcgtcac
     1981 cctgtacgaa ctggtgcccg ccgccggggt caagcacggc cgcgtcgtgg ccctgtccga
     2041 cgacatcgcc cgcagcatgt cggcgcgcgc ctgccgcatc agcgtggttc agggccgtaa
     2101 cgccatcggc atcgaactgc ccaacgccaa gcgcgagacc gtctatctcc gcgacctgct
     2161 ggcctcggcc gaatacgcca aaccggccca catcctgccg ttggcgctgg gtgagagcat
     2221 cggcggcgag ccctatgtcg ccgatctggc gcgcatgccc cacctgctga ttgcggggac
     2281 cac
//
LOCUS       DASVTM010000155        11954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_282842,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000155 DASVTM010000000
VERSION     DASVTM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282842"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="VF633_04330"
     CDS             complement(<1..1660)
                     /locus_tag="VF633_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470317.1"
                     /translation="MMRTSHGFQFALRTGASALAMTLAIAAAAHAQTGPTSAELQTDD
                     ASEVDEVVVTGFRASLASAVSAKRRESGIVDVIKAEDIADFPDLNLAESLQRIPGVTI
                     TRANGEGRQISVRGLGSEYTRVRVNGMEAIATTGGTANSGGTNRSRGFDFNMFASELF
                     NSLTVRKSASADVEEGSLGATVDLQTARPFDYREPTLVLGVQGSYNDLARDVQPRVTL
                     LASRTWADGKFGALFSAAYEKRHILEEGANITRWSHGGSNGATGGFNAASTITGYTAA
                     QINGTLASPGLYHPRIPSYVSYDINSERLGLTGALQFRPDDKTLFTLDVLYSDVKSTR
                     DEAQLQAIGFSRPQATGRPEIIIRSGVVDENRNIVAGVFDNVDLRTQAAHDELETQFQ
                     QYTFTATRNWTDRFRTGFVGGYSRSEFTNPVSTIITFDRANTQGYAYDFRTRAPGITL
                     GFDPTRAANWSMNSGVSEVRIRPSAVTNAFTTAKGYFEFDANDQITLKGGLDYRRFTF
                     DSSGQTRTTETVVQTLTPAQLAAVSTTYSGFGRNFDMPSGNATSW"
     gene            2123..3475
                     /locus_tag="VF633_04335"
     CDS             2123..3475
                     /locus_tag="VF633_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8470318.1"
                     /translation="MTDAAIPIAPATGKRTRVRFLILLLIFLLTTINYADRSTFSIAG
                     DAASRDLGLDPIQMGYILSAFAWAYVLGQIPGGALLDRFGTKLIYAIAIAAWSFFTLI
                     QGFSGFLTGGLVFGALFAMRFAVGLAESPSFPGNARLVAAWFPGSERGTASAIFNSAQ
                     YFSLVVFAPLMGWLVHTFDWHSVFFVMGGLGILAAIVFWKLIHSPVEHPAINQAELDY
                     IEAGGGLIRMEDKVAKKSAFTWPNVKQVITSRMLIGVYIGQYGINVLTYFFVTWFPIY
                     LVKERGLNILEAGFAAAAPALCGFAGGVLGGVISDLLLKKTGSLTIARKTPLLIGMIL
                     ASTIIACVFIQEQWLIIVVMALAFFGKGIASLGWAVVSDTSPKELVGVTGGIFNTVGN
                     TAGIVTPIVIGYIVAATGSFDGALWFVGLHCVLVIAAYFLITGKIERLALKPVVEKAV
                     "
     gene            3475..5325
                     /locus_tag="VF633_04340"
     CDS             3475..5325
                     /locus_tag="VF633_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470319.1"
                     /translation="MHADRRTFLGGTALAALAVAVAPKARAAAALEVTTPMPAPDWAL
                     LQRQLLADNAEACAAFYARYFDERGYFECFERWGANDGPDDAIENVNNWPLLHALGGA
                     DSVKSMYTQAWEGHLRQYTAAKTTEVEIAREGMFYKEFCVQLDWQHHAEELTMFNVQG
                     LSDPYNPRFRDRARRFSAMYMGEDVDAPNYDPQHKIIRSLMNGSRGPMLRKATALDWA
                     GDPFEAGHRFFMEHGEDTYEQTLHHYDEYTDVVGDHPLNLFSTTLAMNAYMLEHEPKY
                     RDWLLGYVDAWADRAKANNDLLPSNVGLDGVIGSAADGNWWGGAYGWGFSPVNPVTGK
                     REDRSRVLRTSIGFFNAYLMTGDDKYLDTWRKQADRLNAAKRTVDGVVQTPTMYGAEG
                     WYGWKDGLHQLNTLDIWWFSMKPSDRDRAPDHPWVAYLEGRNPEYPETALRKDLARVA
                     AMARAEREDTSTADTRLADARLEYNPASVTSLMQTMMGALHIGRPPWNPTTANAGGSP
                     LYARLRYFDADTRRAGVPEDVSALIDRMTADETAVTLVNLSPTQTRSVTIQGGGYGEH
                     QIKTAAYDGATQPVNASAFTLKLAPGAGARVLLTMDRYVNQPTLKFPWDR"
     gene            5504..6721
                     /locus_tag="VF633_04345"
     CDS             5504..6721
                     /locus_tag="VF633_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8470320.1"
                     /translation="MRVRTLTMLAVLAGASFATQASAQTAPARWIAGWTFPPVSNVGP
                     VVPPSPPNPNDVRGPLGPTTVSNQTVSQITTLSASGDRVRIRVSNIYGSAPIRVGAVS
                     LSVGDAAPIAVTFDGSEGLLLPAGAPRLSDPVLLPVKALDRLTLSIFYPDETRLPGHR
                     LRQVMRDGNATAATLAADAPRLRIGALATAIEVETTAATQVIVALGDSITEGTGSLPS
                     GPGGWPERLAQRMVTAGSTWTMVNNGIGGNRLLHQGSGPSALERLDADALAVSGAKCL
                     ILLEGINDIGRPARAEYAHESITADDLIAGYRQVLTRAHAAGLKVVLATIPPFEDANY
                     FTANGEAIRQAANAWIRTSSDVDARVDFDAAVRDPAHPTRLRTEFQSGDWLHPSDAGY
                     AAMVETIPINICD"
     gene            complement(6866..7171)
                     /locus_tag="VF633_04350"
     CDS             complement(6866..7171)
                     /locus_tag="VF633_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEX8470321.1"
                     /translation="MTAQEAAPEFEAADMALPLPTMDMDGDALFRLGMMYSAGQGGCP
                     MDRVSAHMIFNLAAMKGSIEARVYRREMSQEMEREEIAEAQKAARRYIDAGVVTLAA"
     gene            complement(7389..8108)
                     /locus_tag="VF633_04355"
     CDS             complement(7389..8108)
                     /locus_tag="VF633_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8470322.1"
                     /translation="MTRPIELWYWPTPNGWKISIALEEMGLPYETKYVNIGAGEQFTP
                     QFQAISPNGRMPAIIDPDGPDGMPLSIFESGAILQYLGAKSGQFYATDRRHKAEIDQW
                     LFWQVGGLGPMAGQTHHFRQYAPSMIKDQRQLAYGVRRYTNETNRLYGVMDKRLADRP
                     FLAGDYSIADMAAWPWVLPEHQGQNLDDFPNLKDWVERVGARPAVQVGRALGEEMRKN
                     LAASGKAAEDARKVLFGQRAR"
     gene            8346..9383
                     /locus_tag="VF633_04360"
     CDS             8346..9383
                     /locus_tag="VF633_04360"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8470323.1"
                     /translation="MKRSLMVFSAIAAFALPALAPVATLAQDRGQDDSAPTPRRDGGH
                     RNDSNGDRQRTAPDNTAERSESGDRGGRGPDRGGHRGERPDRPSAPASQPNPPQRPDR
                     PDRPGQGEGRPDRPAGGWNNGGPGRPDRPSNGGTNRPDRPGQGRPDQGRPDQGRPDQG
                     RPGNGGWNGNQGRPDRPGQDRPGQGRPDQGRPGNGGWNGNQGRPGGNSGWNNNDNRGG
                     RDRDRQRQEYRSRFDSNRWQQSWDRTHRSDWWRNDNRFRGWNGARIGFYFAPGYGYYS
                     VPRNYYGQTFHEGQYLPSIFWRYSVNDYRTYGLGFPPEGTRWVYVDNSIYLIDQYDGY
                     IIDVIRSAWRW"
     gene            9556..9993
                     /locus_tag="VF633_04365"
     CDS             9556..9993
                     /locus_tag="VF633_04365"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8470324.1"
                     /translation="MRSFAKIAAMAVAALAVTAAPMSASAQSWRGGDRDHDGRYERHE
                     RRADRNRYDGGRYTNRERYRDDRRYYRDQRQDYRRDYRYSQRRYYRGATLPYQYRSNW
                     YIRDYNRYGYAPPPRGYGYYRTDTGDIVVAALATGLIVSLLNN"
     gene            complement(10113..11606)
                     /gene="gatB"
                     /locus_tag="VF633_04370"
     CDS             complement(10113..11606)
                     /gene="gatB"
                     /locus_tag="VF633_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX8470325.1"
                     /translation="MATTTKLIQGRTGPWELVMGLEIHAQVASKAKLFSGAAVGFGAG
                     PNEQVSLVDAGFPGMLPTLNGHCVEQAVKTGLGLKAQINKRSQFDRKNYFYPDLPTGY
                     QISQLYFPIVGEGEVEVEAEDGSFNTVRIERLHLEQDAGKLIHDLSPTESFVDLNRAG
                     TALMEIVSKPDIRSPEEAVAYVKKIRTILMYLGTCDGDMEKGNLRADVNVSVCREGQY
                     AKFQATGDFSHLGTRCEIKNVNSFRFISQAISVEARRQIEIIEDGGKIIQETRLYDPT
                     KNETRSMRSKEEANDYRYFPDPDLLPLEIEQAWIDQIKASLPELPDEKRKRLMSQYGL
                     SQYDAVVLISDQAKADYFEEAAKGRDAKLVANWVTNELSARLSADNLEFDASPLPAAY
                     VAELVALIEEEVISSRIAKEVFDHVWNGEGSPRAVVEARGLVQVNDTGAIEKAVDDLI
                     AANPDKAAAVAEKPQALGWFVGQVMKATGGKANPASVNAILKAKLGL"
     gene            11715..>11954
                     /locus_tag="VF633_04375"
     CDS             11715..>11954
                     /locus_tag="VF633_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470326.1"
                     /translation="MIKTPFAAVAASLMLAGAASAQTTPAAPAAANAPAARPTIESTI
                     ESLINEPATKTVVLKHLPALDQHPAYSQFKGMTLRA"
BASE COUNT         2112 a         4028 c         3671 g         2143 t
ORIGIN      
        1 gccacgacgt cgcgttgccc gagggcatgt cgaagttgcg tccgaacccg ctgtaggtcg
       61 ttgacacggc ggccaactgg gctggcgtca gggtctgcac gactgtctcg gtcgtgcgcg
      121 tctggcccga cgaatcgaag gtgaagcggc gatagtcgag gccgcccttc agcgtgatct
      181 ggtcattggc gtcgaactcg aaatagccct tcgccgtggt gaaggcgttg gtcacggcgc
      241 tagggcgaat acggacctcc gacacaccgc tgttcatcga ccagttggcg gcccgcgtcg
      301 gatcgaagcc cagcgtgatg cccggggcgc gggtgcggaa gtcataggcg tagccctgcg
      361 tgttggcacg gtcgaaggta atgatcgtcg agaccgggtt ggtgaactcc gagcgcgaat
      421 agccgccgac gaagccggtc cggaagcggt cggtccagtt gcgcgtcgcc gtgaaggtat
      481 actgctggaa ctgtgtctcc agctcgtcgt gcgccgcctg ggtgcgcagg tcgacgttgt
      541 cgaacacgcc ggcaacgatg ttgcggttct cgtcgacgac gcccgagcgg atgatgatct
      601 cgggacgacc ggtcgcttgc gggcggctga aaccgatggc ctgaagctgg gcctcgtccc
      661 gggtgctctt cacgtctgaa tacaggacgt ccagcgtgaa cagcgtcttg tcgtcggggc
      721 ggaactgcag ggcgcccgtc aggccgagac gctcggagtt gatgtcgtag ctgacgtagg
      781 acgggatgcg tggatgatag aggccggggc tggccagtgt gccgttgatc tgggcggcgg
      841 tgtagccggt gatcgtagag gcggcgttga agccaccggt ggcgccgttc gaaccgccgt
      901 ggctccagcg cgtgatgttc gccccttcct caaggatgtg tcgcttctca taggcggccg
      961 agaacagggc gccgaacttg ccgtccgccc aggtgcgcga ggccagcagg gtgacgcggg
     1021 gctgaacgtc gcgggccagg tcgttgtagc tgccctgaac gcccagaacg agcgtgggct
     1081 cgcggtagtc gaacggacgt gccgtctgca gatcaaccgt agcgcccagc gagccttcct
     1141 cgacatcggc cgaggcggac ttgcgcaccg tcaggctgtt gaacaactcg gaggcgaaca
     1201 tgttgaagtc gaaaccgcgc gaccggttgg tgccgccgga gttggccgtg ccgcccgtgg
     1261 tggcgatggc ttccatgccg ttgacgcgga cgcgggtgta ttccgaacca aggccacgca
     1321 ccgagatctg acggccctcg ccgtttgcgc gggtgatagt cacacccgga atgcgctgca
     1381 gcgactcggc gaggttgagg tcggggaagt cggcgatgtc ttcggccttg atgacatcaa
     1441 cgatgccgct ttcgcgccgc ttggcgctaa ccgcgctggc gaggctggcg cggaagccgg
     1501 tgacgacgac ttcgtcaact tcggaggcgt catcggtctg cagctcagcg ctggtagggc
     1561 cagtctgggc atgagcggcg gcggcgatcg ccagcgtcat ggccagcgcc gaagcgccgg
     1621 tgcgaagtgc gaactggaat ccgtgcgagg tgcgcatcat gtttccttcc cctcatgccc
     1681 gcattccgcg gatcattatt ggtgacgcct tgggcgccgg ttatccgccg cgcgatgaca
     1741 tcggttggct tcaacccgaa cataggatcg ccccgatgtt cgaggccaat cccaatcggg
     1801 catgaaccaa tgccggagtt gaaccaatgc cgggcctgcg cgcccattcg cgtcatcgat
     1861 cgttcttcgc gtcccatctg atggtatcga tcgatattcg acttgcagca catggtccgg
     1921 atcaatgccg tttctggatc ggtccatagt cggattggtt tggaccgtcg gctcgccccc
     1981 tcgtatcgtc ccacagagcc agacgcccgc aagaggtgtc ggtcggcgcc gccggcgcca
     2041 tgggaagcga acactcaggc gtcggcgtct ccgacatcaa ggttccctcg ccttccacga
     2101 taagtcgagg agcgtaacaa ccatgaccga cgcagccatc cccatcgctc cggcaacagg
     2161 caaacgaacc cgggttcgtt tcctgatcct gctgctgatc ttcctgctga cgacgatcaa
     2221 ctatgcggac cgctcgacct tctccatagc cggcgacgcg gcgtccaggg atctgggcct
     2281 ggatccaatc cagatgggct acatcctctc ggccttcgct tgggcctatg tgctcggcca
     2341 aatcccgggc ggcgccctgc tcgaccggtt tggcaccaag ctgatctacg ccatcgccat
     2401 cgccgcctgg tcgttcttca ctttgatcca aggcttctcc ggcttcctga ccggcggcct
     2461 cgtcttcggc gccctgttcg ccatgcgctt tgctgtcggt ctggccgaga gcccctcctt
     2521 ccccggcaac gcccgcctcg tcgcggcctg gttcccgggg tccgagcgcg gcaccgcctc
     2581 ggccatcttc aactcggccc agtatttctc gctggtggtg ttcgcgccgc tgatgggctg
     2641 gctggtccac accttcgact ggcactcggt cttcttcgtc atgggcggcc tcggcatcct
     2701 cgccgccatc gtcttctgga agctgatcca ctcgcccgtc gaacaccccg ccatcaacca
     2761 agccgagctc gactacatcg aggctggcgg cggtctgatc cgcatggagg ataaggtcgc
     2821 caaaaagtcg gccttcacct ggcccaacgt caaacaggtc atcaccagcc gcatgttgat
     2881 cggcgtctac atcggccagt acggcatcaa cgtcctgacc tatttcttcg tcacctggtt
     2941 cccgatctat ctggtcaagg aacgcggcct gaatatcctc gaggccggtt tcgctgccgc
     3001 cgccccggcc ctgtgcggct tcgcgggcgg cgtcctcggc ggcgtcatct ccgacctgct
     3061 gctcaagaag accggttcgc tgaccatcgc ccgcaagacg cccctgctga tcggcatgat
     3121 cctcgcctcg accatcatcg cctgcgtctt cattcaggag cagtggctga tcatcgtcgt
     3181 catggcgctg gccttcttcg gcaagggcat tgcctcgctc ggctgggctg tcgtctccga
     3241 cacctcgccc aaggaactgg tcggcgtcac aggcggcatc ttcaacacag tgggcaacac
     3301 cgccggcatc gtgaccccca tcgtgatcgg ctatatcgtc gccgcgacgg gcagcttcga
     3361 cggcgccctg tggttcgtgg ggctccactg cgtgctggtc atcgccgcgt atttcctgat
     3421 caccggcaag atcgaacgcc tcgccctgaa acccgtcgtg gagaaagcag tctgatgcac
     3481 gctgatcgca ggactttcct cggcggcacg gcgctcgccg ccctcgccgt agcggtggcc
     3541 cccaaggccc gcgccgctgc cgcccttgag gtgaccacgc ccatgccggc gcccgactgg
     3601 gccctgctgc agcgtcagtt gctggccgac aacgccgagg cctgcgccgc cttctacgcc
     3661 cgctatttcg acgagcgcgg ctatttcgag tgcttcgaac gctggggcgc caacgatggt
     3721 cccgacgacg ccatcgagaa cgtcaacaac tggccgctgc tgcatgcgct gggtggcgcc
     3781 gacagcgtca agtcgatgta cacccaggct tgggaaggcc acctgcggca gtatacagcg
     3841 gcgaagacga ccgaggtcga gatcgcgcgc gagggcatgt tctacaagga gttctgcgtc
     3901 cagctcgact ggcagcacca cgccgaagaa ctgaccatgt tcaacgtgca gggcctgtcc
     3961 gacccctaca atccccgctt ccgcgaccgc gcccgacgct tcagcgccat gtatatgggc
     4021 gaggacgtcg atgccccgaa ctacgatccg cagcacaaga tcattcgcag cctgatgaac
     4081 ggctcgcgcg gtccgatgct gcgcaaggcc accgcgctcg actgggccgg agaccccttc
     4141 gaggccggcc atcgcttctt catggagcac ggcgaggaca cctacgaaca aaccctgcat
     4201 cactacgacg agtacacgga cgtggttggc gaccacccgc tgaacctgtt ctcaaccacg
     4261 ctggcgatga acgcctacat gctggagcac gagccgaaat accgcgactg gctgctcggc
     4321 tacgtcgacg cctgggccga ccgggcaaag gccaacaacg acctgctgcc ctccaacgtc
     4381 ggtctcgacg gcgtcatcgg ctcggcggcg gatggaaact ggtggggcgg cgcctacggc
     4441 tggggcttct cgccggtcaa tccggtcact ggcaagcgcg aggaccgcag ccgcgtcctg
     4501 cgcaccagca tcggcttctt caacgcctat ctgatgaccg gcgacgacaa atatctcgac
     4561 acctggcgca agcaggccga ccgcctcaac gccgccaaac gcaccgtcga cggtgtggtc
     4621 cagaccccga ccatgtacgg cgccgagggc tggtacggct ggaaggacgg cctgcaccag
     4681 ttgaacacgc tcgatatctg gtggttctcg atgaagccct cggaccgcga ccgcgcgccg
     4741 gaccatccat gggtcgccta cctcgaaggc cgcaatcccg aataccccga gacggccttg
     4801 cgcaaggatc tcgcccgcgt ggctgcaatg gccagggccg agcgcgagga cacctcgacc
     4861 gccgacaccc gcctcgccga cgcccggctc gagtacaatc cggccagcgt cacctccctg
     4921 atgcagacca tgatgggcgc gctgcacatc ggccgcccgc cgtggaatcc gaccaccgcc
     4981 aacgccggcg gctcgcccct gtatgctcgt ctgcgctatt tcgacgccga cacacgtcgg
     5041 gccggcgtgc ccgaggacgt ctcggcgctg atcgaccgca tgaccgccga cgagaccgcc
     5101 gtcaccctcg tcaacctgtc accgacccag acccgctcgg tcacgattca gggcggcggc
     5161 tacggcgagc accagatcaa gactgcggct tacgacggcg ccacgcagcc cgtgaacgcc
     5221 tcggctttca cgttaaagct ggccccgggg gcaggggcgc gcgtgctcct gacgatggat
     5281 cgctacgtca atcagcccac gctgaaattc ccgtgggacc gctagggaaa ctctgggacc
     5341 gacaggccaa ccaacctcat aatccgctca tccccgcgaa cgcggggacc cagacctttg
     5401 gcagcggttg ctatcgcgca agcaggcagg tcgcttgatc ccttgcagcg tgcattaggc
     5461 tagaactagg tccccgcttt cgcggggatg agcggagaat aagatgcggg ttcgcacact
     5521 gacaatgctg gctgttttgg ctggcgcatc attcgcaacc caagcgtccg cccagaccgc
     5581 acccgcccgc tggatcgcag gctggacctt cccgcctgtg tcgaacgtgg ggccagtcgt
     5641 tccgccgtcg ccgcccaacc cgaacgacgt gcgcggcccc ctcggcccga cgacggtctc
     5701 gaaccagacc gtcagccaga tcacgaccct ctccgccagc ggcgatcgcg tccgtatccg
     5761 cgtctcgaac atctacggct cggcgcccat ccgtgtaggc gcagtcagcc tctcagtcgg
     5821 agacgcggcg cctatcgccg ttaccttcga cggctccgaa ggcttgctgc ttcccgccgg
     5881 cgccccacga ctgtccgacc cggtcctact cccggtgaaa gccctcgacc gcctcaccct
     5941 cagcattttc tatcccgacg aaacccgcct tcccggccat cgactgcggc aggtcatgcg
     6001 cgacggaaac gccaccgctg caaccctcgc tgccgacgcc ccccgccttc gcatcggagc
     6061 tctcgccacc gccatcgagg tcgaaaccac agccgccacc caagtcatcg tcgccctcgg
     6121 agactcgatc accgaaggca ccggctcgct cccctccggc cccggcggct ggcccgagcg
     6181 gctcgctcaa cgcatggtca cggcggggtc aacctggacc atggtcaaca acggcattgg
     6241 cggcaatcgc ttgctgcatc agggctccgg cccgtccgcg cttgaacgtc tcgacgccga
     6301 cgccctggcg gtctccggcg cgaaatgcct gatcctgctg gagggcatca acgatatcgg
     6361 tcgccccgcg cgcgccgaat acgcccacga aagcatcacg gcggacgacc tgatcgccgg
     6421 ctaccggcag gtcctgaccc gcgcccacgc cgccggtctc aaggtggtcc tcgcgaccat
     6481 tcccccgttc gaggacgcca actatttcac cgcgaacggc gaggccattc gccaggccgc
     6541 caacgcatgg atccgcacct catccgacgt ggacgcccgc gtggacttcg acgccgccgt
     6601 ccgcgaccct gcccatccga cacggctccg caccgagttc cagagcgggg actggcttca
     6661 cccgtccgat gccggctacg ccgctatggt cgaaacgatc ccgataaaca tctgcgacta
     6721 gtcactgggc cctgcgacag acgttaagag agcgcgtccg gtgacgttct ggtcgctggc
     6781 gctagacccg ctggccacca ttccacaccg agatagccaa ggctcccggg cctccgcctc
     6841 tttcctgtag gacaggtggc gaatatcagg cagctaaagt caccacgccg gcgtcaatgt
     6901 agcgacgggc ggctttctgg gcttcggcga tctcttcgcg ttccatttcc tgcgacatct
     6961 cgcggcgata aacgcgcgct tcgatggagc ccttcatagc ggcgagattg aagatcatgt
     7021 gggccgagac gcggtccatc gggcaaccac cctgcccggc cgagtacatc atgccgagac
     7081 ggaacagggc gtcgccgtcc atgtccatgg tcggcagagg cagagccatg tcagcagctt
     7141 caaactcagg cgcggcttct tgcgcggtca tcgtcgtatt cctctggcgg catccattct
     7201 ggagccgttc ttcgaggtca catcaaatac cgtcggtttg aagttaaagt taacggccag
     7261 caggccgaaa aagtctttta tgtaaagagt ttaacaacac agcccgccgt cttcagacat
     7321 catgaacttg ccggtaatcc cgtgtttatc gacatctata caaggttaac ggcgccgggc
     7381 tccgcctgct atcgagcgcg ctgtccgaac aggaccttgc gggcgtcctc agcggccttg
     7441 cccgaggctg caaggttctt gcgcatctcc tcgcccagcg cccgcccgac ttgcaccgca
     7501 ggccgcgcgc cgacccgctc cacccagtcc ttgaggttgg gaaagtcgtc gaggttctgg
     7561 ccttgatgct ctggcagaac ccaaggccag gcagccatat ccgcgatcga atagtcgccc
     7621 gcaagaaacg gcctgtcagc cagccgcttg tccatgacgc catacagccg gttggtctcg
     7681 ttggtgtagc gtcgcacgcc ataagccagt tggcgctggt ccttgatcat gctcggcgcg
     7741 tactgccgaa aatggtgtgt ctggccagcc atcggaccga ggccgcccac ctgccagaac
     7801 aaccactggt caatctcggc cttgtgccgg cggtcagtgg cgtagaactg gcccgacttg
     7861 gcgccgaggt actggaggat ggcgccggac tcaaaaatcg acagcggcat gccgtcggga
     7921 ccgtccggat cgatgatggc cggcatacgg ccattgggac tgatggcctg aaactgcggc
     7981 gtgaactgct cgccggctcc gatgttgacg tacttcgtct catacggcag ccccatctcc
     8041 tcgagggcga tggagatctt ccagccgttc ggcgtaggcc agtaccaaag ctcgatggga
     8101 cgggtcataa agcggtctcc agcgggccga acgcccctta ccgatctggg acctaatcag
     8161 gcgaggcgca acagcaccgg atccaaaagc ctggttgcgc tcggatgggc cattcgcgag
     8221 gccgaggggt gggtctttat tacgaactct tcacgggagc gccccttttc attcagacag
     8281 ggttcatgcg gcggagccga attaaccacc caagccgccc ctgtgggcgt gcggagataa
     8341 cgaccatgaa acgttcactg atggtgttct ctgccatcgc tgccttcgcc cttccggcgt
     8401 tggctcccgt cgcgaccctc gctcaggatc gtggtcagga cgatagcgcg cccacgcctc
     8461 gccgcgatgg cggacaccgc aacgacagca acggcgatcg ccaacggacc gcgccggaca
     8521 acaccgccga gcgcagtgag agcggtgacc gtggcgggcg aggtccagac cggggtggcc
     8581 atcggggcga acgcccggac cgcccgtcgg cgcctgcatc tcaaccgaac ccacctcaac
     8641 gccctgacag acccgaccgg cccggtcagg gtgaaggtcg cccggaccgt ccagccggcg
     8701 ggtggaacaa tggcggaccg ggtcgccctg atcggccctc caatggcggc acgaatcgtc
     8761 ctgaccgtcc cggccaaggc agacccgacc agggacggcc ggaccaaggt cgtccagatc
     8821 aaggccgtcc gggcaatggc ggctggaacg gcaaccaggg ccgtcctgat cgccccggcc
     8881 aggaccgtcc tggccaaggt cgccctgatc aaggtcgtcc cggcaacggc ggctggaacg
     8941 gcaatcaggg tcgccccggc ggcaatagcg gctggaacaa caacgacaac cgtggcggtc
     9001 gcgatcggga tcgccagcgt caggaatatc gcagccgctt cgactccaac cgctggcagc
     9061 agagttggga tcgcacccat cgctccgact ggtggcgcaa cgacaaccgt ttccgcggct
     9121 ggaacggcgc tcgtatcggg ttctattttg cgcccggtta cggctactac agcgtgccga
     9181 ggaactacta cggccagacg ttccacgagg gtcagtacct gccgtcgatc ttctggcgct
     9241 acagcgtcaa cgactaccgg acctatggtc tgggcttccc gcccgaaggt acacggtggg
     9301 tctatgtcga caactcgatc tacctgatcg accagtacga cggctacatc atcgacgtga
     9361 tccgcagcgc ctggcgctgg tagctctcac ggcctgacga cagagcggcc ccttcgaaag
     9421 gaggggccgt tttcgttttt gccgggcctg aagcctgaac cgaacgttca gaaacggttc
     9481 aggcgctaag gttcaattaa cgatcattgg gggtttcgaa gccgctccgt ggcggcagtt
     9541 caaggagatc aagtgatgag aagttttgca aagatcgccg cgatggccgt cgccgcactg
     9601 gctgtcaccg ccgcaccgat gtccgcttcc gcccagtcgt ggcggggcgg cgaccgtgac
     9661 cacgacggac gctatgagcg ccacgagcgt cgcgccgatc gcaatcgcta cgacggcggc
     9721 cgctacacca atcgcgagcg ttaccgtgac gaccgtcgct actatcgcga tcagcgtcag
     9781 gactatcgcc gcgactatcg ctatagccaa cgtcgctatt atcgcggggc gaccctgccc
     9841 taccaatatc ggtcgaactg gtacattcgc gactacaatc gctacggcta tgcacctccg
     9901 ccacgcggat acggctacta tcgcacggac actggcgaca tcgtcgtcgc ggctctggcg
     9961 actgggctga tcgtctcgct gctgaacaac taggcttcac acgccgcaca agaaagagcc
    10021 cgcccggagc gatccgggcg ggcttttcgt tgtgtcacca ggtaccgaac gcgctcaagc
    10081 agcaagccgc cgcgcggcga gttaaagagc ccttaaagac caagcttagc cttcaggatg
    10141 gcgttgaccg acgccgggtt ggccttgccg ccggtggcct tcatgacctg accgacgaac
    10201 cagccgagcg cttgcggctt ctcggcgacg gcggcggcct tgtcggggtt ggcggcgatc
    10261 aggtcgtcga cggccttctc gatggcgccg gtgtcgttga cctgaaccag accgcgcgcc
    10321 tcgacgacgg cgcgaggcga gccctcgccg ttccagacgt ggtcgaacac ttccttggcg
    10381 atccgggacg agatgacctc ttcctcgatc agcgccacca gttcggcgac ataggccgca
    10441 ggcagagggc tggcgtcgaa ctcgaggtta tccgccgaca ggcgggccga aagctcgttg
    10501 gtgacccagt tggcaaccag cttggcatcg cggcccttgg cggcctcctc gaaatagtcg
    10561 gccttggcct gatcggaaat cagcaccacg gcgtcgtatt gcgacaggcc gtactggctc
    10621 atcaaacgct tgcgcttctc gtccgggagt tccggcaggg acgccttgat ctgatcgatc
    10681 caggcctgct cgatttcgag cggcagcagg tcgggatcgg ggaagtagcg atagtcgttc
    10741 gcctcttcct tggaccgcat tgaacgggtc tcgttcttgg tggggtcata caggcgggtt
    10801 tcctgaatga tcttgccgcc gtcctcgata atctcgatct ggcggcgcgc ctcaaccgag
    10861 atggcctgag agatgaagcg gaacgagttg acgttcttga tctcgcagcg cgtgcccaga
    10921 tggctgaagt cgccggtcgc ctggaatttg gcgtactggc cttcgcgaca gaccgagacg
    10981 ttgacgtcgg cgcgcaggtt gcccttctcc atgtcgccgt cgcaggtccc cagatacatc
    11041 aggatggtcc tgatcttctt gacgtaggcg accgcttctt cgggcgagcg gatgtccggc
    11101 ttggagacga tctccatcag cgccgtgccg gcgcggttca ggtcgacgaa gctctcggtc
    11161 ggagacaggt cgtggatcag cttgccggcg tcctgctcca gatgcaggcg ctcgatacgg
    11221 acggtgttga acgacccgtc ctcggcctcg acctcgactt caccctcccc gacgatgggg
    11281 aaatacagct ggctgatctg atagccggtc ggcagatcgg gatagaaata gttcttgcgg
    11341 tcgaactggc tgcgcttgtt gatctgcgcc ttgaggccga gcccggtctt caccgcctgc
    11401 tcgacgcagt gaccgttcag ggtcggaagc atgcccggga agcccgcatc gaccagcgac
    11461 acctgctcgt tggggcccgc gccgaagccc acggccgcgc cggagaacag cttggccttc
    11521 gacgccacct gagcgtggat ctccagcccc atgacgagtt cccaaggccc ggtccggccc
    11581 tggatgagtt tggtggtggt tgcgatcgtg tcggtcatgc gctggtccta gcgccgtcga
    11641 ggacgccgat aaagaggctg tcgtgatgat gtcgcgaaac cacggtcatc tggcggctcc
    11701 caatggagat tccaatgatc aagaccccgt tcgccgccgt cgcggcatca ctgatgctcg
    11761 ccggcgctgc ttcggcccag acgacacccg ccgctccggc cgcagctaac gctcccgcag
    11821 cacgcccgac tatcgaaagc acgatcgaat ccctgattaa cgaaccggcg acgaagactg
    11881 tcgtcctgaa gcacctgccg gccctcgatc agcaccccgc ctacagccag ttcaagggca
    11941 tgaccttgcg cgcc
//
LOCUS       DASVTM010000156         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_919789,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000156 DASVTM010000000
VERSION     DASVTM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919789"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1014)
                     /locus_tag="VF633_04380"
                     /pseudo
     CDS             complement(1..1014)
                     /locus_tag="VF633_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1071..2291
                     /locus_tag="VF633_04385"
     CDS             1071..2291
                     /locus_tag="VF633_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX8470327.1"
                     /translation="MARRKAPHIPDVILDQLLAGADPKTAFEADGLLDRLKKALAERA
                     LNAEMDHHLAGEEAGNSRNGYGRKTVTTETGRIELAIPRDRQATFDPQLIARYQRRFP
                     GFDDKIVSMYARGMSTREIVGHLRELYGIEVSPDLISTVTDAVLEEIAAWQARPLEPV
                     YPIVFFDALRIKVRDEGLVRNKAVHIALGVRANGSKEILGLWLEQNEGAKFWLRVMNE
                     LRNRGVEDVLLAVVDGLKGFPDAIRAVFPETMVQTCIVHLLRHGLDFVSYKDRKAVAV
                     ALKDIYRALDAAAGEAALAAFEAGEWGRKYPAIAQSWRRAWSEVVPFYAFPSEVRRIL
                     YTTNAIEALNATLRRAVRARGHFPTDEAALKLLYLVLNRSEKAWKMGPREWVMAKAQF
                     AVIFGERFTRAMAA"
     gene            2335..2745
                     /locus_tag="VF633_04390"
                     /pseudo
     CDS             2335..2745
                     /locus_tag="VF633_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041359162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          488 a          959 c          965 g          454 t
ORIGIN      
        1 agttgcttgg gagatggaag agagattgcg ccagtgatcg gcgctgcatt ccgcgccagc
       61 catttgcggg cggtgcgccg cgccggcctc gtgcgacgct cggcgacgaa gcggtgaacc
      121 tgccggtgcg tgccggcaaa gccccgctcg cgaagttcgc gccagagttc catcgcgttc
      181 tcgcaccccc ggtcatccgc tgctccagat gcgccagata ggggtcgagg cgactggggc
      241 cggggcgacg tgctgcgcgt tccggaaagc tctcggcctg ggcatacttg cgcaccgtcc
      301 cgcgtgcgag gcccgtcgcc cgggcaatcg ccagcaaggt ctcaccggct tgagagcgcc
      361 gacgcacgtc ctcgtaggcg gccagacggc gggcgcggct gtcaaggctc acggcgatct
      421 cggcttcact ccggcggtag gctcgcagac gttgcccggc tgtcgcccat cttcggaggc
      481 aaggctcggc agacgtcgca agcgtccatg gacgcgggcg agccagcgct ccacggcctg
      541 acgcaggttg aggaggagat gccagcggtc ggcgacctga agggctgcgg gagcgcccat
      601 ggtcgctgca cgcgcgtatt cggacgaacg gtcgcgcgtg atgactgcga taccgggatg
      661 gcaacgcagc cacgcggcga cggtcgtgct ggatcggtcc ggcagcaggt cgatcggacg
      721 tcgccggtcg agatcgacga tgagcgtgcc gtaggtctgt cccttgcgga tcgcccagtc
      781 gtcgattccc accgcccgca gcggaccggt cggcggtagc gtcatgccgt gcacgaggcg
      841 cagcaccgta tcggggctcg tggccatggc cagatgccct gcgagccgag cgccggcttc
      901 gccacccaac gcgagcccag cccgggcctg cgttcgggcg agccgaccgg tgcgtcgtgc
      961 gcgtggcggg agaagccgcg gcagcgattc gacgaaggtg cgccgtcggc agggggactg
     1021 tcaggatttt cgtgtagggg cggccctagt ggacccgaag gaggttcccg atggcacgac
     1081 gcaaagcacc tcacatccct gacgtgatcc tggaccagtt gctggctgga gccgatccta
     1141 agacggcgtt cgaggccgac ggcctgttgg ataggctgaa gaaggccctg gccgagcggg
     1201 cgctcaacgc cgagatggac caccatctgg cgggcgagga ggccggcaac agccgcaacg
     1261 gctacggccg caagacagtg acgaccgaga ccggccggat cgaactggcg atcccccgcg
     1321 accggcaggc gacattcgat ccgcagctga tcgcccggta tcagcgccgc tttcccggct
     1381 tcgacgacaa gatcgtgtcg atgtatgccc gcggcatgag cacccgggag atcgtcgggc
     1441 accttcgcga gctctacggc atcgaggtgt cgcccgacct gatcagcacg gtgaccgacg
     1501 cggtgctgga ggagatcgcc gcctggcagg cccggccact ggagccggtc tacccaatcg
     1561 tgttcttcga cgctctgcgc atcaaggtcc gcgacgaagg ccttgtgcgg aacaaggccg
     1621 tgcacatcgc actcggcgtg cgcgccaacg gatccaagga gatcctcggc ctatggctgg
     1681 agcagaacga gggagccaag ttctggctgc gggtcatgaa cgagctccgg aaccgtggtg
     1741 tcgaagatgt gctgctggcg gtcgtcgacg gactgaaggg cttccccgac gccatccggg
     1801 cggtcttccc cgagacgatg gtccagacct gcatcgtcca tctcctgcgt cacggcctgg
     1861 acttcgtctc ctacaaggat cgcaaagccg tggcggtcgc gctgaaagac atctatcgcg
     1921 cgctcgacgc cgcggctggc gaggcggccc tggctgcctt cgaggcgggc gagtggggcc
     1981 ggaagtaccc ggccatcgcc cagagctggc gacgtgcttg gagcgaggtc gtcccgttct
     2041 acgccttccc cagcgaggtt cggcgcatcc tctacacgac gaacgccatc gaggccctga
     2101 acgccacgct gcgccgagcc gtgcgcgccc gagggcactt cccgaccgac gaggccgcgc
     2161 tgaagctcct ctacctggtc ttgaaccgct ccgagaaggc gtggaagatg ggcccgcgtg
     2221 aatgggtcat ggccaaggcg cagttcgccg tcatcttcgg cgaacgcttc acccgggcca
     2281 tggcggcctg atgttcaacc cgccgcccct acacggaatt tccgatagtc ccccaaggtc
     2341 cacctggcgg tcgacaccct gggctacctc ctggctctgc atgtcacgcc cgccgatgcg
     2401 gacgaccgtg ctcaggtcgg gcagctcgcc gaggcggtgc aggccgccac aggcgatcac
     2461 gtcgatctgg cctacgtcga tcagggctat accggagaga agccggccaa ggcggcccgc
     2521 gcgcacggca tcgaattgga ggtcgtcaaa ctcccggacg ccaagcgcgg cttcgttctg
     2581 ctcccacgcc gctgggtggt cgaacggtcc ttcgcctggg cgacgcgctt tcgccgcctc
     2641 gtcaaagatt acgagcgcta tcccagtacg ctcgcagatc tccacatcgt cgccttcgtc
     2701 tgcatcatgc tcaaacaggc cgctcaactc acaagcggtc cataacagcc tctaacctgg
     2761 cagcctgatt gtcccagccc aagccggaga tcgcggtggc cgcgtcaaag ctacaccacg
     2821 ccaggcgacc atcatgagcg acgttgcaat ttgatgaagc aatcga
//
LOCUS       DASVTM010000157         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_973160,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000157 DASVTM010000000
VERSION     DASVTM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973160"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2475
                     /locus_tag="VF633_04395"
     CDS             <1..2475
                     /locus_tag="VF633_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470328.1"
                     /translation="DEIVVTGQRAAQRAAISVKRNQFEVMDVISSDDIGKLPDHNTAA
                     ALRRIPGVSVQEDQGEPRFPVLRGLTSTYNRTTIDGAIVASVDDTGRTVPLDIVPSVM
                     AGQVQVIKTVTPQNDANAIGGVINITTRSAFDAGRPFFNGMASYGWYEQHGEVRNEDP
                     SYRLSFATGRTFGRNDEWGVVVGASHEQLDYDIPQVESAAPSVREYTAVGAPVNSGSA
                     TGNGIQVPTQVRLFWYNNTKTRDGVNGKLEWRPNADFHWEASALWAHMEDDEERIEYR
                     VEPIGNVTNQTATSGTFASGRGIIGLNQPITKREISLARTAFRWDVTPQWLIDGLLVY
                     SRAKLESPNTSATFQTRDAQGANYGFSYDTSNFFPVFTPTGAGLRTPSNYFMTQFRDS
                     LLETFENTTQVGLNAGFDDGGAERNLKAKFGFNLRSTDREYQNAQTNYVLSTFNFDLV
                     DRPGPTEQIQGGYLLGPRIDVAMLESYRATFARQAPSPRTGDYDLTEDVSAAFGQASY
                     RIGDFTALGGVRYEKTEVSSDTFRTRAGVVTPVQNSGDYDNWLPSVHLRWDALENVVV
                     RAAWTNTIGRPDFASLAGAENVTFDGSLTLVSRGNPGLKPRESEGFDLSAEYYPRDGL
                     ISVAVFSKDIENEIFTATSVEQLDVGAGVQTVTVSTPRNAQTATIRGIELAFQQELYF
                     LPAPFDGFGVSANATFLDTDVSFQNSLGVVRHRSLFLQPSTTTNESIYYQRGPLEAKI
                     SHNYIGGFLETVNDLIPNADQYWKGRHLYDASVSWRFDQGVTVFFEAQNLSNSGRQEN
                     TGPLQRNLQESAEYGRTFWLGMSASF"
     gene            2483..>2727
                     /locus_tag="VF633_04400"
     CDS             2483..>2727
                     /locus_tag="VF633_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470329.1"
                     /translation="MMIVSALFALVLSGAADQAQITAAPAPEHPPIALVGQAETVRRY
                     DANATQGVAVGATDVYAVSNFALTRLDKATGEKKAEWV"
BASE COUNT          516 a          911 c          829 g          471 t
ORIGIN      
        1 gatgagatcg tcgtcaccgg tcaacgcgcg gcgcagcgtg cggccatctc ggtcaagcgc
       61 aaccaattcg aggtgatgga cgtcatctcc tcggacgaca tcggcaagct gcctgaccac
      121 aacaccgccg ccgctctgcg ccgtattccg ggtgtgtcgg tgcaggaaga tcagggtgag
      181 ccgcgcttcc cggtcctgcg cggcctgact tcgacctata accgcaccac catcgacggc
      241 gcgatcgtcg cctcggtgga tgacacaggc cggaccgtgc cgctcgacat cgtgccgtcg
      301 gtaatggccg ggcaggtgca ggtcatcaag acggtgacgc cccagaacga cgccaacgcc
      361 atcggcggcg tcatcaacat cacgacccgc agcgccttcg acgccggtcg cccgttcttc
      421 aacggcatgg catcttacgg ctggtacgag cagcacggcg aggtccgcaa cgaagacccg
      481 tcctaccgcc tgagcttcgc caccggccgc acgtttggta ggaacgacga gtggggcgtc
      541 gtggtcggcg cctcgcacga acagctcgac tatgacatcc cgcaggtcga gtccgccgcc
      601 ccgtcggtcc gtgagtatac ggccgtcggc gctccggtga actcgggctc cgccacaggc
      661 aacggtatcc aggttccgac ccaggtccgc ctgttctggt acaacaacac caagacccgc
      721 gacggtgtga acggaaagct ggaatggcgt ccgaacgccg acttccactg ggaggcgtcc
      781 gcgctctggg cgcacatgga ggatgacgag gaacggatcg aataccgcgt cgagccgatc
      841 ggcaacgtca ccaaccagac agcgacctcg gggaccttcg ccagcggccg aggcatcatc
      901 ggtctcaacc agccgatcac caaacgcgag atcagcctgg cgcgcaccgc cttccgttgg
      961 gacgtgacgc cgcaatggtt gatcgacggc ttgctggtct attcgcgggc caagctggag
     1021 tcgcccaaca cctccgcaac cttccagacc cgcgacgccc agggtgcgaa ctacggtttc
     1081 agctacgaca cgtcgaactt cttccccgtc ttcacgccga ccggggcggg tctgcgcacg
     1141 ccgtccaact atttcatgac ccagttccgc gacagcctgt tggagacgtt cgagaacacc
     1201 acgcaggtcg gcctgaacgc gggcttcgac gacggcggcg ccgagcgcaa tctgaaggcc
     1261 aagttcgggt tcaatctgcg ctcgaccgat cgtgaatatc agaatgctca gaccaactac
     1321 gtgctctcga ccttcaactt cgatctggtc gatcgtcccg gtccgacgga gcagatccag
     1381 ggcggctacc tgctggggcc acgcatcgac gtcgcaatgc tcgagtcgta ccgggccacc
     1441 ttcgcgcggc aggcgccgtc gccccgcacc ggcgactacg atctcaccga ggatgtctcg
     1501 gcagcctttg gtcaggccag ctaccggatc ggggatttca cggccttggg tggagttcgt
     1561 tacgagaaga ccgaggtttc ctcggacacg ttccggaccc gcgcgggtgt ggtgacaccc
     1621 gttcagaact cgggtgacta tgacaactgg ttgccgagcg tgcatctgcg ttgggatgct
     1681 ctggagaatg tcgtcgtccg cgccgcctgg accaacacga tcggtcgacc cgatttcgca
     1741 tcgctggccg gtgcggagaa cgtgacattc gacggatccc tgacgctggt ctcgcgaggc
     1801 aatccgggat tgaagccgcg cgaaagcgaa ggcttcgacc tgtcggccga atactacccg
     1861 cgcgatggcc tgatatcggt cgcggtcttc tcgaaagaca tcgagaatga aatcttcacg
     1921 gcgacctccg tcgaacagct cgacgtcggg gcaggtgtgc agacggtcac cgtgtcgacg
     1981 cctcgcaacg cccagacggc cactatccgg ggcatcgaac tggcgttcca gcaggagctc
     2041 tacttcctgc ctgcgccgtt cgacggcttc ggcgtcagcg ccaacgcaac cttcctcgac
     2101 accgacgtct cgttccagaa cagtctgggc gtggttcgtc accgctcctt gttcctgcag
     2161 ccgagcacga cgaccaacga gtcgatctac taccagcgcg gtccgctcga agcgaagatc
     2221 tcgcacaact acatcggcgg cttcctcgag acggtgaacg acctgatccc caacgccgac
     2281 cagtactgga agggccgcca cctctatgac gcctcggtca gctggcgctt cgaccaaggc
     2341 gtgacggtct tcttcgaggc ccagaacctg tcgaactcgg ggcgtcagga gaacacgggc
     2401 ccgctgcagc gcaacctgca ggagtcagcg gaatacggcc ggaccttctg gctcggcatg
     2461 tcggccagct tctaggaacc ggatgatgat cgtttcagcc ctgttcgctc tcgttctgtc
     2521 gggagctgcc gaccaagccc agatcacggc agctcccgcg cctgagcatc cgcccatcgc
     2581 tttggtcggg caagcagaga cggtgcgccg ctatgacgcc aacgctaccc aaggcgtcgc
     2641 cgtaggcgcc acggatgtct atgcggtgtc caacttcgcc ctgacccgct tggataaggc
     2701 gacgggcgag aagaaggccg aatgggt
//
LOCUS       DASVTM010000158         4889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_229604,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000158 DASVTM010000000
VERSION     DASVTM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229604"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VF633_04405"
     CDS             <1..693
                     /locus_tag="VF633_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8470330.1"
                     /translation="TSHADQADWIFCLVRTDPDAPKHLGISFVLFDMATPGVSTRPIT
                     LISGKSPFCETFFDDVRVEKVNLVGTLNRGWDVAKALLAHERTMIGAMGSLGSGRPMG
                     QVAADSIGVDTDGRLDDPMLRARIAELEVDLAAFHLSMERVGDQVKARQASPAIASML
                     KYYGSELNKRRHELAMAAGGSDALEWESERSHHGRVARDWLRTKANSIEGGTSEVQLN
                     IIAKHLLQLPGA"
     gene            698..1816
                     /locus_tag="VF633_04410"
     CDS             698..1816
                     /locus_tag="VF633_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8470331.1"
                     /translation="MLILTEEQTMLKEAADGFLSENGPVAHLRSLRDSNDADGVSREL
                     WKAFGEMGFAGVIIPEEHGGSGLGAVEAGVVAEALGRTLTPSPFMGSSILCAKILTDG
                     GSPEQQAAWLPRIAAGEAILSLAIDEGAKHRPSNIATTAERSGNGFKLNGSKGFVLDG
                     HIADALIVAAHSDEGLSLFLVDPKAAGVVIERTVMVDAHNAARVTLTDVQVDADAVIG
                     AVDHGKTLLQGALNLGRACAAASLTGAGDQAFQITLDYLRTRKQFGKLIGEFQALQHR
                     AAHLFTEIELARAAVIGALQRLDAGKDPDGLAVSVAKAKAGRVAELAVQEAVQMHGGV
                     GMTDAFDVGLYMKRVRVLNELLGDAGFHSERLARAQGY"
     gene            1899..2261
                     /locus_tag="VF633_04415"
     CDS             1899..2261
                     /locus_tag="VF633_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470332.1"
                     /translation="MRILLAALALTAGLLAGCTPMSAPDPAVPGQSVDMASCTARGGE
                     IRRVGRMQSEQCIVKYADAGKQCTSGSQCAGDCLIQGNSGVAPGAPVTGQCQVDSDRF
                     GCNTRVEDGKAAATLCVD"
     gene            complement(2330..2596)
                     /locus_tag="VF633_04420"
     CDS             complement(2330..2596)
                     /locus_tag="VF633_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470333.1"
                     /translation="MRKMILAAVATFALAGVAACGESAADKTAETKADALEAQAAATP
                     NEQTEKNLNAQADHIEQNAGNADGGMTTANTPSTSPTAGETAGH"
     gene            complement(2737..3714)
                     /gene="glk"
                     /locus_tag="VF633_04425"
     CDS             complement(2737..3714)
                     /gene="glk"
                     /locus_tag="VF633_04425"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX8470334.1"
                     /translation="MTVAWDKTLLVGDVGGTNARFALAHMVEGKPVLEHHDSFPAERY
                     PTFLEGVKAFIDGCDVKPTGGVIAVAGPVTDGEIDLTNSPWAVSEKQLATLGLDPVRL
                     INDFEALAWGAPVVPADQLASLGGPEEGELHAAVAVLGPGTGFGVSALVRDIHGAEMA
                     MPSEGGHACFPPGDSVEDEILRILRRRYDRVSIERLICGPGLLNMHRALAEIDGRETH
                     IDDPAQITETALADPNSHCGATLARFCAILGAVAGDIALTTGARGGVYIAGGIAPRIL
                     PFLKASPFRERFERKGRFQEYMQAIPTNVILHKHAALLGAARVAFADIS"
     gene            complement(3711..>4889)
                     /locus_tag="VF633_04430"
     CDS             complement(3711..>4889)
                     /locus_tag="VF633_04430"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX8470335.1"
                     /translation="PGTCTFYGTANSNQMMMELGGLHMPSTAFVHPDTGLRDALTAAA
                     AKRAVELARSGECRMADVISEKSIVNMIVALLATGGSTNHAIHLVAMAKAAGVLIDWT
                     DMDQLSSVTPLLARVYPNGSADVNAFQAAGGVAYVARELAQAGHIHSDVTTIMGEGIQ
                     AYFQEPSMVDGELVWREGVTESLDLDILRPASDPFDTEGGLRLVQGDLGRAVIKISAV
                     KPENRIVEAPAEVYETQEDVLADFKAGKLDRDVIVVLRFQGPRANGMPELHSLSPALG
                     VLQDRGFKVALVTDGRMSGASGKTPAAIHVSPEALAGGPLAHVQTGDVIRLDAEAGVL
                     TTIAVADLQARPRARRSAANREASTWGYGRELFSAFRRVVTSAEEGATVCYSGEEEDA
                     "
BASE COUNT          844 a         1534 c         1641 g          870 t
ORIGIN      
        1 acctcccacg ccgatcaggc cgactggata ttctgtctgg tgcgcaccga cccggacgcg
       61 cccaagcacc tcggcatctc gttcgtgctg ttcgacatgg cgacgccggg ggtctcgacc
      121 cgtcccatca ccctgatcag cggcaagtcg ccgttctgcg agaccttctt cgacgatgtg
      181 cgggtcgaga aggtgaacct cgtcggcacg ctgaaccggg gctgggacgt ggccaaggcg
      241 ttgctggcgc acgagcgaac catgatcgga gccatgggct cgctaggatc cggtcggccg
      301 atgggccagg tggccgccga cagcatcggc gtcgacaccg atggccggtt ggacgatccg
      361 atgctccgtg cgcggattgc tgagctagag gtcgatctcg ccgccttcca tctatcgatg
      421 gagcgggtcg gggatcaggt gaaggcgcgt caggccagtc cggcgatcgc ttcgatgctc
      481 aaatactacg gctcggagct caacaagcgc cgccacgaac tggcgatggc ggctggcggt
      541 tccgacgcgc tggaatggga gagcgagcgt tcccatcacg gcagggtggc gcgcgactgg
      601 ttacggacca aggccaattc gatcgagggc gggaccagcg aagtgcagct gaacatcatc
      661 gccaaacacc ttctgcaact gcccggagcc tgagcggatg ctgatcctga ccgaagaaca
      721 gacaatgctg aaggaggccg ccgatggctt cctgagcgaa aacgggccgg tcgcccacct
      781 gcggtccctg cgcgatagca acgacgccga cggcgtttcg cgtgaactct ggaaggcgtt
      841 cggcgagatg gggttcgcgg gcgtgatcat cccggaggaa catggcggct ccggcctcgg
      901 cgccgtcgag gccggcgtgg tggccgaggc gctgggtcgt acgctgaccc cctcgccgtt
      961 catgggctca agtatcctgt gcgcaaagat actgactgac ggagggtctc ccgaacagca
     1021 ggcggcctgg ctgccgagga tcgcggcagg cgaagctatc ctgtcgctgg cgatcgacga
     1081 gggcgccaag caccgtccat cgaacatcgc cacgaccgcc gaacgctcgg gcaacggctt
     1141 caagctgaac ggctcgaagg gcttcgtgct ggacggtcat atcgccgatg ccctgatcgt
     1201 cgcggcccac tccgacgagg gcctcagcct gttcctcgtc gatcccaagg ctgcgggcgt
     1261 ggtaatcgaa cgcaccgtca tggtcgacgc ccacaatgcc gcgcgcgtca ccctgaccga
     1321 tgttcaggtc gatgcggacg ccgtgatcgg cgctgtcgat catggaaaga cgctgcttca
     1381 gggggcgctg aaccttggtc gggcctgcgc tgcggcttca ctgaccggag ccggcgatca
     1441 ggctttccag atcacgctcg actacctgcg cacgcgcaaa cagttcggca agctgatcgg
     1501 cgagtttcag gcgctgcaac atcgcgcggc ccacctgttc acggaaattg agttggctcg
     1561 ggctgccgtg atcggcgcgc tccagcggct ggatgccggt aaggaccccg acggtctggc
     1621 cgtctcggtc gccaaggcca aggccggtcg ggtcgccgaa ctggcggttc aggaggcggt
     1681 gcagatgcac ggcggggtcg ggatgacgga cgcgttcgac gtcggcctct atatgaagcg
     1741 ggtgcgagtg ctcaacgagt tgctgggcga cgcaggtttc cattccgaac gactggcccg
     1801 ggctcagggg tactgatgca tcgggcgatg gtgtcacgaa acggacccat cccctcccta
     1861 ggcagtttca gaactgaaat ccgattcgga gactttccat gcgcatactg ctcgccgctc
     1921 tcgccttgac cgctggcctt ctcgccggct gcaccccgat gagcgcgccg gatccggctg
     1981 tccccggtca gagcgtggac atggcgtcct gcaccgcgcg gggtggcgaa atccgccggg
     2041 tcggacgcat gcagagcgag caatgcatcg tcaaatacgc cgacgccggc aaacagtgca
     2101 cctcaggctc gcaatgcgcg ggcgactgcc tcatccaagg caacagcggc gtggctcccg
     2161 gcgcgcccgt gaccggccaa tgccaggtcg acagcgaccg cttcggctgc aacacccgtg
     2221 tcgaagacgg caaggcggct gccacactct gcgtcgactg atcgaacgga gagaccacat
     2281 agaaaagccc ccgccgtttg cacggcgggg gcttctttat tgccttggct tagtggccgg
     2341 cggtttcgcc agcggtcggc gaggtgctgg gcgtgttggc ggtggtcatg ccgccatcgg
     2401 cgttgccagc gttttgttcg atgtggtcgg cctgcgcgtt caggttcttc tctgtctgct
     2461 cgttaggcgt tgcagccgct tgggcttcga gagcgtcagc cttcgtttcg gcggtcttgt
     2521 cggcagcgct ttcgccgcag gcggccacgc cggccagagc gaaggtggcg acggcagcaa
     2581 ggatcatctt acgcattggg tattcccttc catgttggag actatcaaac gtagccattc
     2641 caatccggtt cacgcccgcg tgataatttt ttctgccagc cacctctgta ttatatcggt
     2701 ttttgatcgg gactttcaca aatgacggtt ttgggatcag ctgatatcgg cgaaggcgac
     2761 gcgggctgcg cctagcaagg ccgcgtgttt gtgcaggatg acgttggtcg gaatggcctg
     2821 catgtattcc tgaaaccgcc cttttcgctc aaatcgctcc cggaaggggc tggccttgag
     2881 gaagggcagg atgcgcggag cgatcccacc ggcgatgtag acgccgccgc gcgcgccggt
     2941 ggtcagggcg atatctccgg ccacggcccc gaggatggcg cagaaccggg ccagtgtcgc
     3001 gccgcagtgg ctgttgggat cggccagggc ggtttcggtg atctgtgccg gatcgtcgat
     3061 atgggtctcg cgtccgtcga tttcggccaa agcgcgatgc atgttcaaca ggcccggacc
     3121 acagatgaga cgctcgatcg atacccggtc atagcgtcgg cgcaggatcc tcaggatttc
     3181 gtcctctacc gaatcaccgg gaggaaagca ggcgtgaccg ccctcggacg gcatggccat
     3241 ctcggcgccg tggatgtcgc gcaccagagc ggagacacca aagcccgtgc ctgggcccag
     3301 taccgcgacg gcggcgtgca gctcaccctc ttcggggccg cccagcgaag ccagctggtc
     3361 agccgggact acaggcgctc cccaggccag cgcctcgaag tcattgatca gccgcaccgg
     3421 gtcgagcccc aacgtagcga gctgcttttc agacacggcc cagggcgaat tggtcaggtc
     3481 gatctcgcca tcggtcacgg gcccagcgac ggcgatgact ccgccggtcg gcttgacgtc
     3541 gcaaccgtcg atgaaagcct tcacgccctc gaggaaggtc ggatagcgtt cggcggggaa
     3601 gctgtcgtgg tgctccagca ccggctttcc ctcgaccata tgcgccaagg cgaagcgggc
     3661 attggtgccg cccacgtcac cgacgagaag ggtcttgtcc caggctacag tcatgcgtct
     3721 tcttcttccc cggagtaaca gactgtggcg ccctcttcgg cggaggtgac cacgcggcgg
     3781 aacgccgaga acagttcgcg accatagccc caggtagagg cctcgcggtt ggctgctgac
     3841 cggcgggctc tgggacgagc ctgcagatcg gcgacggcga tcgtggtcag gaccccggct
     3901 tcggcgtcca gacggataac atcgccggtt tgtacatggg cgagcggacc gcccgccaga
     3961 gcttcgggtg agacgtggat agctgcgggc gttttgcccg aagcgcccga catccggccg
     4021 tcggtgacga gggcgacctt gaagccccgg tcctgcagca cgcccagggc gggcgacagg
     4081 gagtgaagct caggcatgcc gttggcgcgc ggaccctgga accgcagcac gacgatgacg
     4141 tcgcggtcca gctttccagc cttgaagtcg gccagcacgt cttcctgcgt ctcatagact
     4201 tcagctggag cctcgacgat ccggttctct ggcttgaccg ccgagatctt gatcacagcc
     4261 cggcccagat cgccctgtac cagacgcaag cccccctcgg tgtcgaacgg atcggacgcg
     4321 ggtcgcagga tgtccaggtc gaggctttcg gtgacgccct ctcgccagac cagttcgccg
     4381 tcgaccattg agggttcctg gaaataggcc tgaatgccct cgcccatgat cgtcgtgacg
     4441 tcggagtgga tatgtccggc ctgcgccagc tcgcgagcaa cgtaggccac gccgccggca
     4501 gcctggaagg cgttcacgtc ggccgagccg ttcggataga cgcgggcgag cagcggcgtc
     4561 accgacgaca gctgatccat gtcggtccag tcgatcagca cgccggcggc tttggccatg
     4621 gcgaccagat ggatggcgtg attagtcgag ccgcccgtgg ccagcaaggc cacgatcatg
     4681 ttgacgatcg acttctcgga gatgacgtcg gccatgcggc actcgccgct gcgggccaat
     4741 tcgacggccc gcttggcggc tgcggcggtg agggcgtcgc gcaggccggt gtcaggatgg
     4801 acgaaggcgg tcgaaggcat gtgcaaaccg ccgagttcca tcatcatctg gttggagttg
     4861 gcggtgccgt agaaggtgca tgtgccggg
//
LOCUS       DASVTM010000159         4046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_815776,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000159 DASVTM010000000
VERSION     DASVTM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815776"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="VF633_04435"
     CDS             complement(<1..1637)
                     /locus_tag="VF633_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX8470336.1"
                     /translation="MFKSITAVLHPRLSSAASAVVLAIAFASPAHAEWLRAETEHFII
                     YGDTNRSEITRYATKLERFDSLLRTYFPIPVENMIPKLEIVLADGGGDMNRMSPGIGS
                     SVAGFYTPDSGRIFAVVNTRSEMDDVVIFHEYAHHFMFQMTAPAYPSWFVEGFAEYFA
                     QSDVRPDHLRVGGISSGRMRSLAVPANQWAPTADVLSWRISASGRYRGFDYYAQSWAL
                     THYMMADEGRKRKLGEYLNLVKNGASSIEAFQTAFGRTPAQLQEDLRRYLLLGSVNVM
                     TPQIELRDTPVAVTEMPPSYGRLVWFDFRLDRNGYKPAEAPEDATEDVRRRTERRNRE
                     AQEEQQTLIREALAEADLHPGDRMGLMVKSRAQRLAGHPSQAFDTLMPLLTATSTDPI
                     SLRLAGEILRETAQTQTDQAAKLAMMRQSSAYLARAMDADPLDFRIYLALDDNRQDSP
                     GYPNANDLSTLEVASALAPQSSDARVRTARAYLAHDQPALAVIMLMPVVNNPHGGTDL
                     KATRALLAQARAAAGMAPTLTDAPPPEDNGVEEGDGD"
     gene            complement(1726..2106)
                     /locus_tag="VF633_04440"
     CDS             complement(1726..2106)
                     /locus_tag="VF633_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470337.1"
                     /translation="MLFLLNDVVFDLDEATPVTPADARRFEKLGFDYVLELGCELFAE
                     DPMLHRTDPGRARRLAWLIAERSPEINAALFAAPGQNCDPDLVEPRFCALPETVMRQL
                     GRRGEKGQLDAVAADRVVWNRMAA"
     gene            complement(2235..2570)
                     /locus_tag="VF633_04445"
     CDS             complement(2235..2570)
                     /locus_tag="VF633_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEX8470338.1"
                     /translation="MLLSSDLWVSALIRRAEIGGGNATVARRGDQRAGTVIVKAYNTR
                     ERTARLYTEAFGQDGERLWIQPVTSEFESELDAYIQRQIGYDPDIWVVEIEDRDGRHF
                     ITETVAKED"
     gene            2666..3499
                     /locus_tag="VF633_04450"
     CDS             2666..3499
                     /locus_tag="VF633_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470339.1"
                     /translation="MLVAVTLAVPFTALADATAPYYERSFVLAADAKCRLFTPILTNA
                     LSASTWQARGAAARAGVSDLSLAQTVERAKVNAANTKCADPQLATVRTRVATAFSGWS
                     RIARMTFPGGRSSWVANRSDFSSPTWRLMQTTRVGATPVTFGYVEGGAERQNLKAVVS
                     FAGRSRPYAARILMRNVERAPHAFLGDGGVAMMPPESTRQAFFAAGNLPAERALLQPE
                     ETQGEAWLFPPATAEAMAKLDPREPFVVEFLFGDGSVAKATFETGDFAAGRAFIAMGS
                     L"
     gene            complement(3502..>4046)
                     /locus_tag="VF633_04455"
     CDS             complement(3502..>4046)
                     /locus_tag="VF633_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470340.1"
                     /translation="SLERFDARDVVSAAIALVRLSATDKGVDLNGVLPIAPLMVDADR
                     RALKQMTLNLLSNAIKFTHAGGSITVTADAIGPYLEVVVADTGVGVAPEDLRRLGRPF
                     EQAGGAEQRAQGTGLGLSLVRALSELHGGRMSIDSTLGEGTAVTVRMPVVSLARPASP
                     EGGAEIIPLRPDFSSANDAG"
BASE COUNT          713 a         1300 c         1304 g          729 t
ORIGIN      
        1 tgatctccat cgccttcctc tacaccgttg tcctccggcg ggggagcatc agtgagcgtc
       61 ggcgccattc ccgctgccgc cctggcctgg gccagcagcg cgcgcgtggc tttcaagtca
      121 gttccgccgt ggggattgtt gaccacaggc ataagcatga tcaccgcgag cgctggctga
      181 tcgtgagcca gataggcgcg ggcggtgcgg acgcgggcgt cggatgactg gggcgccagg
      241 gccgacgcga cttccaaagt gctcaggtcg ttggcgttag gataccccgg cgagtcctgg
      301 cggttgtcgt cgagcgccag atagatgcgg aaatccagcg gatcggcgtc catcgcccgc
      361 gccagataag ccgagctttg acgcatcatc gcgagcttcg ccgcctgatc agtctgggtc
      421 tgtgcagttt cccgaaggat ttcgcccgcc aaccggagtg atatcggatc cgtcgaagtg
      481 gccgtcagca gcggcatcag ggtgtcgaag gcttgcgagg gatgaccggc caggcgctgt
      541 gcacgagact tcaccatcaa ccccatccga tctcctggat gaagatcagc ctcagccaag
      601 gcttcccgga tcaaggtctg ctgctcctcc tgagcctcgc ggttacgtcg ctcggtgcgg
      661 cgtcggacat cttcggtggc gtcttcgggc gcctctgccg gcttgtaacc attgcggtcg
      721 agacggaaat cgaaccagac gagccggccg taggatggcg gcatctcggt caccgctacg
      781 ggggtgtcgc gcaactcgat ctggggcgtc atcacattga ccgagcctag aagaagatag
      841 cggcgcaggt cttcctgaag ctgagccgga gttctgccga acgctgtctg gaaggcctca
      901 atcgagctcg cgccgttctt gaccagattc aggtactcgc ccagtttccg cttccggccc
      961 tcgtccgcca tcatgtaatg ggtcagggcc caggactggg cgtaatagtc gaacccccgg
     1021 tagcgaccgc tcgccgaaat acgccagctc aacacgtcag ccgtcggcgc ccactggttc
     1081 gcggggaccg ccagagacct cattcttccg ctgctgatcc ctccgacccg aaggtggtca
     1141 ggacgcacat ccgactgagc gaaatattcg gcgaagcctt cgacgaacca ggacggatag
     1201 gccggcgccg tcatctggaa catgaaatgg tgggcgtact cgtggaagat cacgacgtcg
     1261 tccatttcgc tgcgagtgtt gaccaccgcg aaaatccgac ccgagtccgg cgtgtagaat
     1321 cctgccacgc tcgagccgat ccccggcgac attcggttca tgtcgccgcc accatcggcc
     1381 aagacgattt ccagcttcgg gatcatattt tcgacaggga tcgggaaata ggtgcgcagc
     1441 aggctgtcga accgctccag cttggtggcg tagcgggtga tctcgctacg gttcgtgtca
     1501 ccataaatga tgaaatgctc ggtttcggct ctcaaccatt cagcgtgggc tggcgaagcg
     1561 aaagcgatcg ccagcactac ggcacttgcg gcgctggata gccgtggatg caggactgcc
     1621 gtgattgact tgaacatccc ggtcttcccc cgaccacaac ccaaggcggc agtcttggac
     1681 gcagaacggt cggcgtcaag cgcccaggca acctggactg tgaagtcacg ccgccatgcg
     1741 gttccacacg acgcgatcgg cggcgacggc atccagttgg cctttttcac cgcgccggcc
     1801 aagctgacgc atcactgttt ccggcagggc gcagaagcgg ggctccacca gatcgggatc
     1861 acaattttgg cctggggcgg cgaacagggc ggcgttgatt tcggggctgc gctcggcgat
     1921 cagccaagcg agccggcggg cgcggccggg gtcggtccgg tgcagcatcg ggtcctcggc
     1981 gaacagctcg cagccgagtt ccagcacata gtcgaagccc agtttctcga accggcgcgc
     2041 gtcggcgggc gtgaccgggg tggcctcgtc cagatcgaac acgacgtcgt tcagaagaaa
     2101 cagcatgagg cctactcgcc agacatcgac gtttctcgcc gacatccgga cactaggccg
     2161 gctcgcttga ggtcccgttc acgaacgcgg ttaagcgttt ctaaccaggg aaggcctcct
     2221 cgcgcaggaa gagactagtc ctccttggcc accgtctccg tgatgaaatg ccgcccgtcg
     2281 cggtcctcga tctcgaccac ccagatgtcg gggtcgtaac cgatctggcg ctggatgtag
     2341 gcgtcgagct cgctctcgaa ctcgctggtg acaggctgga tccacaaacg ttcgccgtct
     2401 tgcccaaagg cttcggtgta gagcctggcc gtgcgctcgc gggtgttgta ggccttgacg
     2461 atcacggtcc cggcgcgctg gtcgccacgc ctcgcgactg ttgcgttgcc cccgccgatt
     2521 tcggcgcggc ggatcagggc gctgacccag aggtcgctgg aaagaagcaa atcgctaacc
     2581 ttgctcggaa acgggaccgg aaccccgatg gttctacggt aggttcatga ggataggcct
     2641 tccgacccga tacgccagaa caaccgtgct cgtcgcggtg accctggccg ttccgttcac
     2701 tgcattggcc gacgccacag ccccctatta cgagcgcagc ttcgtgctgg ccgccgatgc
     2761 gaaatgccgg ctgttcacac ccatcttgac gaacgccctg tccgcctcga cctggcaggc
     2821 tcgcggcgca gcggcgcgcg caggcgtcag tgacctgtcg ttggcccaga cagtcgaacg
     2881 agccaaggtt aacgcagcca acacgaagtg cgccgatcct caactggcga cggtgcggac
     2941 gcgcgtggcc acggcctttt caggctggtc gcgcatcgcc cggatgacct tcccgggcgg
     3001 gcggtcgtca tgggtcgcca accgcagcga tttctccagt ccgacatggc ggttgatgca
     3061 gacaacgcgc gtcggcgcca cgccggtcac cttcggctat gttgagggcg gagccgaaag
     3121 gcaaaacctc aaggccgtgg tgtccttcgc cggtcggtcg cgtccctatg cggcgcggat
     3181 cctgatgcgt aacgtcgaac gtgcgccgca cgcttttctg ggcgacggcg gggtggcgat
     3241 gatgcccccg gaatcgacgc ggcaagcctt cttcgctgcc ggcaatctgc ctgcggaacg
     3301 ggcgctgcta cagccagaag agacgcaagg cgaggcgtgg ctgttcccgc ctgcgacggc
     3361 agaggccatg gcgaaactgg acccgcgcga gccgttcgtc gtcgagttcc tgttcggcga
     3421 cggtagcgtg gcgaaggcga cgttcgagac aggcgacttc gccgccggtc gggccttcat
     3481 cgccatgggg tcgctgtaga tctagcctgc gtcattcgca gagctgaaat ccggacgcaa
     3541 cgggatgatt tccgcaccgc cttctggcga ggccggtcgc gccaggctga cgacgggcat
     3601 gcgcaccgta acggcggtgc cttcgccgag cgtgctgtcg atgctcatcc tgccaccgtg
     3661 cagttccgac agcgcgcgca ccagtgacag cccgagcccg gtgccctggg cacgctgctc
     3721 ggccccgccc gcctgttcga agggccggcc cagccggcgc agatcctcag gcgcgacgcc
     3781 cacaccggta tcggccacga cgacctccag ataggggccg atggcgtctg cggtgacggt
     3841 tatggagcct ccggcgtggg tgaatttgat ggcgttcgac agcaggttca gcgtcatctg
     3901 tttcaacgcc cggcggtcgg cgtcgaccat cagcggcgcg attggcagga cgccgttcag
     3961 gtccacgccc ttgtcggtcg ccgacaaccg gacgagggcg atagcggcgg agaccacgtc
     4021 acgagcatcg aaccgctcca gtgaca
//
LOCUS       DASVTM010000160         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_973432,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000160 DASVTM010000000
VERSION     DASVTM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973432"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VF633_04460"
     CDS             <1..917
                     /locus_tag="VF633_04460"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470341.1"
                     /translation="EYDDTLPALNVAFEPIENLQIRFAAAKVIARPPVTNLIPVFTLS
                     ALNAPNPSASLGNTQLEPYRAKTYDVSVEWYFAPESLLSFAYFYKDISTAIQITQQNL
                     TYAQLTALNPAAFPAGGGRDPNLPYVYSTPTNTPGGPLKGFEISYQQPFSFLPGMLSN
                     LGTQLNYTHVESELQYCTTASCGAFVVADLVNLSPNAWNATLYYDDGKLNARISAAYR
                     DDYIQLVPAPNAGSRGIAVTGKTETTSIDASASYKLTDELTLTLEGINLTDEPNRQYH
                     GDLGGERNSTYVYQHTGRQIFVGARYKF"
     gene            1133..1558
                     /locus_tag="VF633_04465"
     CDS             1133..1558
                     /locus_tag="VF633_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470342.1"
                     /translation="MLIALPLLLIPVVLYNIVILFGATGGGGMAEADAILRDPLFSIP
                     MTSGANWSVGVGDLILFLSLILLFFELLKSTSSQKVAIVNHALSLIVFVVCLVEFLLI
                     RGFATSTFFLIVSMVMLDVLAGFIVTIIAARKDLDFGGS"
     gene            complement(1572..1808)
                     /locus_tag="VF633_04470"
     CDS             complement(1572..1808)
                     /locus_tag="VF633_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470343.1"
                     /translation="MASKDSSAPVLQILPDYVAEVRKVAENQRLSFTVADGQDGASEF
                     RFQRLSDDALFALLEDVPDEAYADRAITGDPWAP"
     gene            complement(1829..2395)
                     /gene="hslV"
                     /locus_tag="VF633_04475"
     CDS             complement(1829..2395)
                     /gene="hslV"
                     /locus_tag="VF633_04475"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX8470344.1"
                     /translation="MKEFNASDDNSSPGWHGTTILAVRKNGRTVIAGDGQVSMGATIV
                     KGAARKVRVLAGGKVLAGFAGATADAFTLIERLEAKLEQYPDQLARACVDLAKDWRTD
                     RYLRRLEAMLLVADASSIFTVTGVGDVLEPEQGVAAVGSGGNYALSAALALLDNSDLD
                     AEAIARKSMAIAASICVYTNGNLTVETL"
     gene            2578..2970
                     /locus_tag="VF633_04480"
     CDS             2578..2970
                     /locus_tag="VF633_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX8470345.1"
                     /translation="MYVSDAVGTAGSSILSIAQILGASDVHNRRDDLKGLLIFHRGQF
                     MQLIEGPRTDLDRLLKRLGADPRHENMRMMVNGPAEGAPLGALPMVQVSVAGELEGMI
                     GERTLDRLSLRQVESIFATAATMGRLAA"
     gene            3002..3748
                     /gene="moeB"
                     /locus_tag="VF633_04485"
     CDS             3002..3748
                     /gene="moeB"
                     /locus_tag="VF633_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX8470346.1"
                     /translation="MAHFSQDEVERYARHLVLSEVGGPGQQKLARARVLIVGAGGVGG
                     PASLYLAAAGVGTIGLVDADTVSLSNLQRQIQFATTDIGRPKVAASAERLGAINPHVV
                     LRTHAVMLAADTAAALIADYDIVLDGTDDFATRFAVNAACVMAGVTLVSGALGRWDGQ
                     VAVFEGRPCYQCLVSEAPPDAETCSRVGVIGALAGVIGAMAALEVVKLITGAGEPLTG
                     RLLLYDGLHATARTVKIAADPSCPICALKA"
     gene            complement(3755..>4099)
                     /locus_tag="VF633_04490"
     CDS             complement(3755..>4099)
                     /locus_tag="VF633_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8470347.1"
                     /translation="PATRDTHHLLNAERLARLQPHAILINTARGELIDEAALSEAMAR
                     RAIYGVGLDVFENEPAIHAGLLGHSNVVLLPHLGSATLEARQDMGDRVILNVMTYQNG
                     HRPPDRVIPAML"
BASE COUNT          723 a         1364 c         1241 g          771 t
ORIGIN      
        1 atgagtacga cgacaccctg ccggccctca acgtcgcgtt cgagccgatc gagaacctgc
       61 aaatccgctt cgccgccgcc aaggtcatcg ctcgtccgcc ggtcacaaac ctgatcccgg
      121 tattcacgct cagcgccctg aacgctccga acccctcggc gtcgttgggc aatacccagc
      181 tggagccgta ccgcgccaag acctacgacg tgtccgtcga atggtatttc gcgccggaat
      241 cgctcttgtc cttcgcctat ttctacaagg acatttcgac cgctatccag atcacccagc
      301 agaacctgac ctacgcccag ctgacggctc tcaaccctgc agctttcccg gccggcggtg
      361 gacgcgatcc caatctgccc tatgtctatt caacgccgac gaacacgcca ggtggcccgc
      421 tcaagggatt cgagatcagc taccagcagc cgttctcgtt cctgcccggt atgctgtcga
      481 acctcggcac gcagctgaac tacactcacg tcgagtccga gcttcagtac tgcacgaccg
      541 cgagctgtgg cgcctttgtc gtcgcggatc tggtgaacct ttcgcctaac gcctggaacg
      601 ccacgctcta ctatgacgac ggcaagctga acgcccggat ctcggcggct tatcgcgacg
      661 actacatcca gctagttccg gcgccgaacg ccgggtcgcg cgggatcgcc gtcacgggca
      721 agaccgaaac gacctcgatc gacgcctcgg cttcctacaa gctgaccgac gaactgacgc
      781 tgacgctcga agggatcaac ctgaccgacg agccgaaccg ccagtatcac ggcgacctcg
      841 gcggcgaacg caactcgacc tacgtctacc agcacaccgg ccgccagatc ttcgtcggcg
      901 cccgctacaa gttctagacc cgccgaagtc tggcttcaga ccaagcgacc agaggcccga
      961 tcgcttgcgg tcgggcctct tttcatgccc gctgcagggc ccggcctgaa gaggcggcgc
     1021 tctcgttcgc ctgtcaggcg agcgggcgtg gcggactgct gtaatccggg atatgtgtca
     1081 tcgttaagaa gctcagccgc ttgaccggca tccgcttgag agaaccgctg tcatgctgat
     1141 cgccctgccc ctgctgctga tcccggtggt gctctacaac atcgtcatcc tgttcggggc
     1201 gaccggcgga ggcggtatgg ctgaagccga cgcaatcctg cgcgatccgc tattctcgat
     1261 cccgatgacc tcgggcgcca actggagcgt gggcgttggc gatctgatcc tgttcctgtc
     1321 gctgatcctg ctgttcttcg aactgctgaa gtcgacatcg agccagaagg tcgcgatcgt
     1381 caatcacgcc ctgtcgctga tcgttttcgt cgtctgtctg gtcgagttcc tgctgatccg
     1441 cgggttcgcg acctcgacct tcttcctgat cgtgagcatg gtcatgctgg acgttctggc
     1501 cgggttcatc gtcaccatta tcgcggcccg taaggatctg gacttcggcg gctcgtaagc
     1561 ctcgacgtcc gtcagggagc ccacggatcg ccggtgatgg cgcgatccgc ataggcctca
     1621 tcaggaacgt cttcgagcaa ggcgaacagg gcgtcgtcac tcaaacgctg aaagcggaac
     1681 tccgacgcgc catcctgacc atcggcgacg gtgaacgaca gacgctggtt ctcggcgacc
     1741 ttgcggacct cagcgacata gtccggcagg atctgaagca ccggtgcgga cgagtctttc
     1801 gaggccataa gaccctcctg caatctgact agagggtctc tacagtgaga ttcccgttgg
     1861 tatacacgca gatgctggcg gcgatggcca tggatttgcg cgcgatcgct tcggcatcca
     1921 ggtctgagtt atccagcagg gccagcgccg cagacagcgc atagttgccg cccgacccaa
     1981 cggccgccac gccttgctca ggctccagca cgtcgccgac cccggtgacg gtgaagatcg
     2041 agctggcgtc ggcgaccagc agcatggcct ccagacggcg caggtagcgg tcggtgcgcc
     2101 agtccttggc caggtcgacg caggcgcgcg cgagctggtc cggatactgc tccagcttgg
     2161 cctccagccg ctcgatcagg gtgaaggcgt cggcggtggc tccggcgaaa cccgccagca
     2221 ccttgccgcc cgccaggacg cgcaccttgc gcgccgcgcc cttgacgatg gtcgcgccca
     2281 tggagacctg accgtcgccg gcaatgaccg tccgaccatt cttgcggacg gccagaatgg
     2341 tggtgccgtg ccaaccgggg ctcgagttgt cgtcggaagc gttgaactct ttcatggatc
     2401 tcagatggcg tcggcgaagc caaccatcaa gcacgggcac aagattctaa ctttgctctc
     2461 gtgagacaca gttttgaccc acgtcaccgc tatcagccgc acccttcgtt ctgcgtgcct
     2521 ggcgttcctc ccaaaaaatc tcacctctaa aaaggcctcg ccctgttaca gcgcgcgatc
     2581 tatgtcagcg atgctgtcgg tacggcaggg agttcgatcc tttcgatcgc ccaaattctc
     2641 ggcgcgtccg acgtccataa ccgacgcgac gacctgaaag gcctgctgat ctttcatcgc
     2701 ggccagttca tgcaactgat cgagggaccc cggacagatc tggatcgcct gctcaagcgt
     2761 ctaggcgcgg atcctcgtca cgagaacatg cggatgatgg tcaacggtcc agccgaaggg
     2821 gcccctctcg gggccctgcc catggtccag gtcagcgtgg cgggtgagct cgaggggatg
     2881 atcggggaac gcacgctcga ccggctgtcc ctgcgtcagg tcgaatctat tttcgcgact
     2941 gctgcgacca tgggcaggct cgccgcctga caatcggccc cgcgacgcct agatgtcgcg
     3001 tgtggcccat ttttcccaag acgaagtcga acgttacgct cgccatctgg tgctgtccga
     3061 ggtcggcgga ccgggacagc agaagctcgc ccgcgcccgc gtgctgatcg tcggggctgg
     3121 cggcgtaggc ggtccggcct cgctgtatct tgcagcagcg ggcgtcggga cgatcggcct
     3181 cgttgatgcc gacaccgtct ccctgtccaa tctgcagcgt cagatccagt tcgctacgac
     3241 cgacattggc cgacccaagg tcgcagcaag cgccgaacgc ctgggggcga tcaatccgca
     3301 cgtcgtcctc cggacccacg ccgtgatgct ggcagccgac acggctgcag cgctgatcgc
     3361 cgactacgat attgtgctgg acggcaccga cgactttgcc acccggttcg ccgtcaatgc
     3421 cgcctgcgtc atggcggggg tcacgctggt gtccggcgcc ctcggccggt gggacggaca
     3481 ggtcgccgtg ttcgaaggcc gcccctgcta tcagtgcctc gtctctgaag ccccgcccga
     3541 cgccgaaacc tgctcacgcg tcggcgtgat cggcgccttg gcgggcgtaa ttggagcaat
     3601 ggctgcgctg gaagtcgtga aactgatcac cggagcaggc gagccgctaa ccggacgcct
     3661 gctgctttat gacggtctgc acgctacggc ccgtaccgtg aagatcgcgg ccgatccgtc
     3721 atgccccatc tgcgcgctga aggcctgacg cggcctagag catcgccggg atgacgcggt
     3781 ccggaggacg gtggccgttc tgataagtca tgacgttgag gatcacccgg tcgcccatgt
     3841 cctgacgggc ctccagcgtg gccgaaccca gatgcggcag aagcaccacg ttggaatgac
     3901 cgagcaggcc tgcgtggatg gccggttcgt tctcgaacac gtccagccct acgccgtaga
     3961 tcgcccgtcg agccatggct tcggacaggg cggcttcgtc gatcaactcg ccgcgcgcag
     4021 tgttgatcag gatggcgtgc ggttgaagcc gggccagtcg ctcggcattc agcaggtgat
     4081 gggtgtccct ggtcgccgg
//
LOCUS       DASVTM010000161         6143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_865583,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000161 DASVTM010000000
VERSION     DASVTM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865583"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VF633_04495"
     CDS             complement(<1..589)
                     /locus_tag="VF633_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8470348.1"
                     /translation="MTKTYDLIVRGGEVCNHAGRGLADVGITDGRIEFVGDLSQASSG
                     ETFDATGLTVLPGVIDTQVHFREPGLEWKEDLESGSRSAALGGVTAVFEMPNTEPSTT
                     DPDALADKLVRARDRMWTDHAFYIGGTHGNSSMLSELERLPGCCGVKVFMGASTGTLL
                     VADDDGVREVLRNVKRRATFHSEDEYRLVERRPLAR"
     gene            complement(656..1612)
                     /locus_tag="VF633_04500"
     CDS             complement(656..1612)
                     /locus_tag="VF633_04500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX8470349.1"
                     /translation="MTARFPILYVAEAEAEAAVLSSGVLSYLVEAMPQATFTVVGSPA
                     TSPLFADTPRLDRLLTLDRDGRFEWITLWNQVRDIKWGLVVDMRGSTLSGKLRRHKRA
                     VRGKDDPELHAVAQAADVLKLDEIPAPRLYFGEETQGTADALVKPDGKPILAVGPGTD
                     WIGKRWPADRYSRVVSALLADDGPLAGGRLLIVGEEADRDLAHTIRLAVARNRVIETQ
                     GRLTRLQSAAALSHASLYVGADSIWTQLAVAAGVPAVGVFGPSDDARVGPWQGVSVRG
                     PRSLEEFRTLDPRLNQAIQHMMDLPAERVLKAAKKLLAAKKA"
     gene            1738..2553
                     /locus_tag="VF633_04505"
     CDS             1738..2553
                     /locus_tag="VF633_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX8470350.1"
                     /translation="MTHIAHLDSRALIRVSGDDVKAFLHNLLTQDVETLGEGELRFGA
                     LLSPPGRLLFDLFLFGEPGAVVLDVAAERRDALIQRLTMYKLRAKVEIIADERPVVAS
                     WPEIADGFIADPRTTLLGGRHYGDAPVTASEADFDAHRLAVGVPDPARDMAVDRTYPI
                     EADFDLLNGIDFAKGCFVGQETTSRMKRRGTIKNRMLPIAFDGPAPEIGAEVLLNGDR
                     RAGEVMTGADGSAIALLRLDRIDGVLTVDGRPVTVRTPAWMPVEPAPPAEPID"
     gene            2595..3182
                     /locus_tag="VF633_04510"
     CDS             2595..3182
                     /locus_tag="VF633_04510"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX8470351.1"
                     /translation="MTESPHRCAWCGTDPLYTAYHDTDWGVPEYDSRALWEKLVLDGF
                     QAGLAWITILRKRDTMRAAFAGFDPVKVAQFDDADRQRLMEDPGIIRSRAKIESAIRG
                     AHIYLAMQASGEDFSEWLWSFVGGVPIQGERRGMGDVPTQTEVSVAMAKALKKRGFNF
                     CGPVIVYAFMQATGMVNDHIESCFRCEEVRAMGGE"
     gene            3251..3850
                     /locus_tag="VF633_04515"
     CDS             3251..3850
                     /locus_tag="VF633_04515"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX8470352.1"
                     /translation="MALEILLSQTVGGPVCGVDEAGRGPWAGPVSAAAVILNPDDLPE
                     GIDDSKVLTEKRRFELEPRIKACAVAWGVGFASVEEIEELNILHATGLAMCRAIEALQ
                     VHPSAALVDGNYRFKLPCDVKTVVGGDGLSLSIAAASILAKTARDRLMIELDVEYPGY
                     GFASHKGYNAPIHQEALKTLGPCPAHRPSWAPIRALLEG"
     gene            complement(3847..4107)
                     /locus_tag="VF633_04520"
     CDS             complement(3847..4107)
                     /locus_tag="VF633_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470353.1"
                     /translation="MTPISHAEAQRLQAADRAYRKSADALDTVAWRAPSANRFDGRPA
                     NDVAAHDPSLTEMPAVPVWLSVAAGAVAAAAMGALLGGALHI"
     gene            complement(4167..5219)
                     /locus_tag="VF633_04525"
     CDS             complement(4167..5219)
                     /locus_tag="VF633_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX8470354.1"
                     /translation="MIKYLGSKRALLDPIKAAVAGALPDAGMVCDLFSGSARVGHALK
                     GQGYRVWSNDHNAYAHTLATAYVQADHEVWHDRAAAVIAELSEVKPEAGWFTKTFCED
                     ARFFHPENGALIDAMRDRIAAMALEPELEAIALVSLMGAADRVDSTAGLHMAYMKAWA
                     PRALKPLSLRMPDLLPGVPGGPCKATQGDALEIATQFDGDLVYLDPPYNQHSYLGNYH
                     VWESLVLWDKPQTYGVANKRIDVRTRKSAFNSKPGIGPALGAVIEAIRAPNLIVSFND
                     EGYLSRAQLVEMLSARGHVRVVEIDRPRYVGARIGIHNPKGEKVGAIGRLKNVEYLFV
                     VTDRVIGGVGEIPEAA"
     gene            complement(5250..>6143)
                     /locus_tag="VF633_04530"
     CDS             complement(5250..>6143)
                     /locus_tag="VF633_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX8470355.1"
                     /translation="CIGVDWVALSFVQKPEDMAELKRLVGGKAACLAKIEKPQGLSDL
                     EAILDYCDGVMVARGDLGVEMDPEEVPVAQKAIIRAARRRGVPVIVATQMLESMTGSP
                     APTRAEASDVANAVYEGADALMLSAETASGDYPLEAVGIMNRIMERVEADPIWPSLMD
                     AEHAGMDDFDADALVAAAGKAAQAQSTACIVVFTTLGGTARRMSRERPLQPILALTPK
                     PETARRLSLVWGLEPRFGKEPTSLEDVTEDAVAGAVEFGLAQPGQRVLILAGTPFGAP
                     GAANLLRLAHAPSKPVKRSKG"
BASE COUNT         1056 a         2101 c         1962 g         1024 t
ORIGIN      
        1 tgcgggccag aggccgacgc tcgaccagac ggtattcgtc ttcggagtgg aaggtcgcgc
       61 gccgcttcac attgcgcagc acctcacgca cgccgtcgtc gtcggccacc agcagcgtgc
      121 ccgtcgaggc gcccataaag accttgacgc cgcagcagcc gggcaaccgc tccagctcgc
      181 tcagcatgct cgaattgccg tgcgtcccgc cgatatagaa ggcgtggtcg gtccacatcc
      241 ggtcgcgcgc ccggaccagc ttgtcggcca aggcatcggg gtcggtcgtc gaaggctcgg
      301 tattcggcat ctcgaacacg gcggtcaccc cgcccagcgc tgctgaacga gatccgctct
      361 cgaggtcttc cttccactcc agtcccggct cgcggaaatg aacctgggtg tcgatcacgc
      421 ccggcagcac cgtcagcccc gtggcgtcga acgtctcacc ggacgacgcc tgcgacaggt
      481 cgccaacgaa ctcgatccgc ccgtcggtga tgccgacgtc ggccagaccg cgcccggcgt
      541 gattgcagac ctcgccgccc cgcacgatca ggtcataggt cttggtcatc ggacaactcc
      601 tcgggggcga cggctcgcat atgcagaaca gcaggtcccc gggtaaaccc cggtctcagg
      661 ctttcttcgc cgccagcagt ttcttcgcag ccttcagcac ccgttcggcg ggcaggtcca
      721 tcatatgctg gatcgcctga ttgagccggg gatcgagcgt cctgaactct tcgagcgagc
      781 gcggcccgcg cacagacacc ccctgccagg gcccgacgcg agcatcgtcg gacggcccaa
      841 acacgcccac agccggcacg cccgcagcta cggccaactg ggtccagatc gagtcagcgc
      901 cgacgtacaa gctggcgtgg gacagggccg ccgctgactg cagccgggtc agccggcctt
      961 gagtctcgat cacccggttg cgggccacag ccagacggat ggtgtgggcc agatcgcggt
     1021 cagcttcctc gccgacgatc aacaaccgac caccggccag aggaccgtcg tcggccagca
     1081 atgccgaaac aacgcgggaa taccggtcgg cgggccagcg cttgccgatc cagtccgtcc
     1141 ccggaccgac ggcgagaatg ggcttgccgt caggcttcac cagagcgtcg gcggtgcctt
     1201 gcgtctcctc gccaaaatac agacgcggcg ccgggatctc gtcgagcttg agcacgtcag
     1261 cggcctgagc caccgcatgg agttcgggat catctttccc gcgcaccgcc cgcttgtgac
     1321 gcctcagctt acccgacaag gtcgagccac gcatgtcgac gactagcccc cacttgatgt
     1381 cacgaacctg attccacagg gtgatccatt cgaaccggcc gtcgcgatcc agcgtcagca
     1441 accggtcgag gcgcggcgtg tcggcgaaca gcggcgacgt cgcgggcgat ccgaccacgg
     1501 tgaacgtcgc ctgcggcatg gcttcgacca gataggacag cacgccggac gagagcacgg
     1561 cggcctcggc ctcggcctct gcgacataca aaatggggaa acgcgccgtc atggttcgac
     1621 cgtatacgga ttctcttgtg ggttcagagg gatgaatgac ggaactcggt ctgtctggtg
     1681 gaaaactaat gtctagcggg gctcgctccc tgaacgcacc aaccctatct tggccgcatg
     1741 acccacatcg ctcatctcga cagccgcgcc ctgatccgag tgtcgggtga cgatgtaaaa
     1801 gcctttctgc acaacctgtt gacccaggat gtggagacgc tgggtgaagg cgaactgcgg
     1861 ttcggcgccc tgctgtcgcc gccgggacgg ctactgttcg acctgttctt gttcggcgaa
     1921 cccggggccg tcgtgctgga cgtggcggct gagcggcgcg acgccctgat ccagcgcctg
     1981 accatgtaca agctgcgcgc caaggtcgaa atcatcgccg atgagcgacc cgtggtggcg
     2041 agctggcccg agatcgcgga cgggttcatc gctgacccgc gcaccactct gctcggcgga
     2101 cgccactatg gcgacgcgcc tgtcaccgcg agcgaggctg acttcgacgc tcatcggctg
     2161 gctgtcggcg tccccgatcc cgcgcgcgac atggccgtcg accggacgta tccgatcgag
     2221 gccgacttcg acctgctgaa cggcatcgac ttcgcaaagg gctgtttcgt aggtcaggag
     2281 acgacctcgc ggatgaagcg gcgcggcacg atcaagaacc gcatgctgcc gatcgccttc
     2341 gacggtcccg cgcccgagat cggcgccgag gtgctgctga acggcgaccg ccgcgcgggc
     2401 gaggtcatga ccggcgccga cggtagcgcc atcgccctgc tgaggctcga ccgcatcgac
     2461 ggcgtcctga ccgtcgatgg ccgtccggtg actgtgcgca cgcccgcgtg gatgccggtc
     2521 gagcctgcgc cgccggctga accgatcgac tgacgcgact tccgccgtct ccgggcgggg
     2581 gttaggatcg gccgatgacc gaatctccac atcgctgcgc ctggtgtggg accgacccgc
     2641 tctacacggc ctatcacgac accgattggg gcgtgcctga gtacgactcg cgcgccctgt
     2701 gggagaagct ggtgctggac gggtttcagg cggggctagc ctggatcacc atcctgcgga
     2761 agcgcgacac gatgcgcgcg gctttcgccg ggttcgatcc cgtgaaggtc gcgcagttcg
     2821 acgacgctga ccgtcaacgg ttgatggaag acccgggcat catccgctcg cgcgccaaga
     2881 tcgagagcgc catccggggc gcacacatct atctcgccat gcaggcctcg ggcgaagact
     2941 tctccgaatg gctctggtcg ttcgtcgggg gcgttccgat acagggagag cggcgcggca
     3001 tgggcgacgt gccgacccag accgaggtct cggtcgccat ggccaaggcg ctgaagaagc
     3061 gcggcttcaa cttctgtggc ccggtgatcg tctacgcctt catgcaggcg accggcatgg
     3121 tcaacgacca catcgagagc tgcttccgct gcgaagaagt tcgggctatg ggcggcgaat
     3181 gaccggcaag atcgccaaac cgaaagcagc ctcaaaggcg actacgaagg ccgaccgcgc
     3241 cttcccgacc atggcgctcg aaatcctgct gtcccagacc gttggcggtc ccgtctgcgg
     3301 cgtggacgaa gccggacgcg ggccttgggc ggggccggta tctgcggcgg cggtgatcct
     3361 gaaccccgac gacctgcccg agggcatcga cgactccaag gtcctgaccg agaagcggcg
     3421 gtttgaactg gagccccgga tcaaggcctg cgccgtggcc tggggtgtcg gcttcgcctc
     3481 ggtcgaggag atcgaggaac tcaacatcct gcacgccacc ggcctcgcca tgtgccgcgc
     3541 catcgaggcc ctgcaggtcc atccgtctgc ggcgctggtc gatggcaact atcgcttcaa
     3601 gctgccctgc gacgtcaaga cggtagttgg tggcgacggc ctctcgctgt cgatcgcggc
     3661 ggcctcgatc ctggccaaga cggcgcggga tcggctgatg atcgaactgg atgtggaata
     3721 cccgggctac ggtttcgcca gccacaaggg ctacaacgcg ccgatccatc aggaagcgct
     3781 caagaccctc ggcccctgcc ccgcacaccg ccctagctgg gcgccgatcc gggctctgct
     3841 cgaaggctag atgtgcagcg cgccgcccag cagcgcgccc attgccgcgg ctgcgactgc
     3901 acctgctgcg accgatagcc aaaccggtac ggcaggcatc tccgtcaatg atggatcatg
     3961 ggccgcgacg tcattggcag gacgaccgtc gaagcggttc gcactcggcg cgcgccaggc
     4021 cacggtgtcc agggcgtcgg cgctcttgcg ataggcccgg tctgcggcct gcaaacgctg
     4081 ggcttcggca tgcgatatcg gggtcatcaa tggctcctcc tgccgagcaa aacggcctga
     4141 gtccgctacc gttcccttca cgcaaatcag gccgcttccg gtatctcccc aacgccgcca
     4201 atcacccgat ccgtaaccac gaacagatat tcgacgttct tcagccggcc gatcgcaccg
     4261 accttctcgc ccttgggatt atggatgccg atccgggcgc cgacgtagcg cgggcggtcg
     4321 atctccacga cccggacatg ccctcgcgcc gacagcatct cgaccagctg cgcgcggctc
     4381 agatagcctt cgtcgttgaa cgatacgatc aggtttggcg cccggatcgc ctcgatcacc
     4441 gcacccagcg ccggaccgat gccgggcttg gagttgaagg cgctcttgcg cgtgcgcacg
     4501 tcaatccgct tgttggcgac gccataggtc tgcggcttgt cccacagcac caggctctcc
     4561 cagacgtggt agttcccaag gtaggaatgc tggttgtagg gcgggtccag atagacgagg
     4621 tcgccatcaa actgggtggc gatttccagc gcatcgcctt gcgtcgcctt gcacggcccg
     4681 ccggggacac ccggtaggag gtcgggcatc cgcagcgaga gcggcttcaa cgccctcggc
     4741 gcccaggctt tcatataggc catgtgcaga cctgcggtgg aatccacccg gtcggccgcg
     4801 cccatcaggg acacgagcgc aatcgcctcc agctcaggtt ccagcgccat cgccgcgatg
     4861 cgatcgcgca tggcgtcgat cagggccccg ttttccggat ggaagaaccg agcgtcctcg
     4921 cagaaggttt tggtgaacca gccagcctcc ggcttgacct cggacagctc ggctatgacg
     4981 gcggcggcgc ggtcgtgcca gacctcgtga tcagcctgca cataggccgt ggccagtgtg
     5041 tgagcatagg cgttgtggtc gttagaccag acccgatagc cctgcccctt cagagcatgg
     5101 ccgacgcgcg cactgccgga gaacaggtcg cagaccatgc ctgcgtcggg gagcgcgccg
     5161 gccacagccg ccttgatcgg atccagcagg gcccgtttcg agccgagata tttgatcatg
     5221 acggccctcc cctttttgtc gggaaggcgt cagcccttgc tgcgcttcac cggtttggag
     5281 ggagcatggg ccaatcggag cagattcgca gcccccggcg caccgaacgg cgtgccggcc
     5341 agaatcagca ccctctgacc cggctgagcc aagccgaatt ccactgcccc ggcgacggcg
     5401 tcctcggtaa cgtcctcgag tgaggtcggc tccttgccga aacgcggttc cagtccccag
     5461 accagcgaca accgtcgcgc cgtctcgggc ttgggcgtca gggcgaggat cggctgcagc
     5521 gggcgttcgc gcgacatccg ccgtgcggtc ccgcccagcg tcgtgaacac cacgatacag
     5581 gcggtcgact gggcctgagc cgccttgccc gccgccgcga ccaacgcatc ggcgtcgaaa
     5641 tcgtccatgc cggcgtgttc tgcatccatc aggctcggcc agatcggatc ggcttcgacc
     5701 cgctccatga tccggttcat gatgccgaca gcttccagcg gatagtcgcc cgaagcggtt
     5761 tcggccgaca gcatcagggc gtcggcgcct tcatagacgg cgttggcgac gtcggacgcc
     5821 tcggctcggg tcggtgccgg agacccggtc atagactcca gcatctgggt cgcgacgatg
     5881 actggcacgc cacgccggcg ggcggcgcgg ataatggcct tctgggccac cggcacttcc
     5941 tcaggatcca tctcgacccc gagatcgccg cgcgcgacca tgacgccgtc gcagtagtcg
     6001 aggatggcct ccagatcgct caagccttgc ggcttctcga tcttggccag acaggccgcc
     6061 ttgccgccga ccagccgttt cagttcggcc atgtcctcgg gcttttgaac gaagctgagc
     6121 gccacccaat ccacgccgat aca
//
LOCUS       DASVTM010000162         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_387219,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000162 DASVTM010000000
VERSION     DASVTM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387219"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /gene="nagE"
                     /locus_tag="VF633_04535"
     CDS             complement(<1..1489)
                     /gene="nagE"
                     /locus_tag="VF633_04535"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HEX8470356.1"
                     /translation="MKIGLSSLQPLGRALMLPIAVLPVAGLLLRLGQPDLLDIAFLAA
                     AGQAVFANLGLLFAMGVAVGFARDNNGAAGLAGVVCFLIATSGAKSLIDIPAEVVTGM
                     TEAAGKLAGAAFKDSAIARLGVPIGILSGLIAGDFYNRFSTLKLPDYLAFFSGRRFVP
                     IVSGLAGLALALVIGLTFSGLDHGIDVFSRAIVGSGEVGLFVFGVLNRLLIVTGLHHI
                     LNNVAWFIVGDYGGATGDLNRFFAGDPSAGAFMSGFFPVMMFGLPAACLAMYHTARPE
                     RKKAVGGMLASLALTAFLTGVTEPIEFTFMFLAPVLYALHAVLTGLAMVIMSLLDSHL
                     GFTFSAGLFDYVINFGQATRPWLLLPVGLLYALIYYGLFRFFIERFDLKTPGREPATE
                     GLSTAPALGTTGSKTEDRAIGFIVALGGAANLRSVAACTTRLRLVINARDKIDEPALK
                     ALGARGVIYPSGDDLQVILGPIADAVADEIKIALARGPAAAVDGDV"
     gene            complement(1486..>2461)
                     /locus_tag="VF633_04540"
     CDS             complement(1486..>2461)
                     /locus_tag="VF633_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX8470357.1"
                     /translation="ARAFAAEPCRTADGVRIEVFANLGAASETVGAVVGGAEGCGLLR
                     TEFLFLDRQTAPDEAEQLSQYQAIADGLSGRPLVVRTLDAGGDKPLPYLPMPHEDNPA
                     LGLRGVRSGLLHPELLLTQMRAVCRVQSSGLVALMLPMIASVAEVRQARALLDRAVAD
                     AGRPAPLLGVMIETPAAAMTTDLLAAEIDFVSIGTNDLTQYTLAMDRQNPHLAVQLDG
                     LHPAVLRLIAASAAGAADLQWIGVCGGLASDRLAAPILVGLGVTELSAAPAVVAEIKA
                     TVRGFTLGECQALAATALKLDSAEAVRRVANEALMRNRTPRQSAGAAA"
BASE COUNT          474 a          828 c          809 g          350 t
ORIGIN      
        1 cgacatcgcc gtccacggcc gccgccggtc cccgcgcgag cgcaatcttg atctcatccg
       61 ccactgcgtc ggcgatgggg ccgaggatga cctggagatc gtcgccggat ggatagatca
      121 cgccccgggc ccccaaggcc ttgagcgcgg gctcgtcgat cttgtctcgc gcgttgatga
      181 cgagtctcag ccgcgtggtg caggccgcga ccgatctgag gttcgccgcc ccacccagcg
      241 ccacgatgaa cccgatggct cgatcttcag ttttcgaacc ggtggttccg agcgccggcg
      301 ccgtggacag gccctccgta gccggctcgc gtccgggggt tttcaggtcg aaccgttcga
      361 tgaagaagcg gaacaggccg taatagatta gcgcgtagag cagtccgacg ggaagaagca
      421 gccacgggcg cgtcgcctga ccgaagttga tgacatagtc gaacagacct gccgagaagg
      481 tgaagccgag gtgtgagtcc agcaggctca tgatcaccat cgccagcccc gtgaggacgg
      541 cgtgcagggc gtagagcacc ggcgccagaa acatgaaggt gaactcgatc ggctcggtca
      601 ccccggtcag gaacgccgtc agcgccagcg acgccagcat gccgccgacg gcctttttcc
      661 gctcagggcg ggccgtgtgg tacatggcca gacaggcggc gggcaggccg aacatcatca
      721 cgggaaagaa gccgctcatg aaggcgccgg cgctcggatc gcctgcgaag aagcggttca
      781 ggtccccggt cgcgccgccg tagtcgccga cgatgaacca ggcgacgttg ttgaggatgt
      841 gatgcagacc ggtgacgatc aacagccggt tcagcacccc gaacacgaac aggccaacct
      901 cgccggaccc gacgatagcc cggctgaaga catcgatgcc gtgatcaagc ccggagaatg
      961 tcagaccgat gacgagggcc agtgcgaggc ccgccagtcc cgagacgatc ggcacgaacc
     1021 gccgtccgct gaaaaacgca agatagtccg gtagcttgag agtggagaag cggttgtaga
     1081 aatcccccgc gatcaggccg gacagaatgc cgatcggcac acccagccgc gcgattgcgc
     1141 tgtccttgaa agccgcgccg gccagcttgc ccgccgcctc ggtcatgccc gtgacgacct
     1201 cggccgggat gtcgatcaga gacttggcgc cggacgtcgc aatcaggaag cagacgacgc
     1261 ccgctagtcc cgccgccccg ttattgtcgc gtgcaaagcc cacggccacg cccatggcga
     1321 acagcagacc gaggttcgcg aacacggctt ggccggcggc ggccagaaag gcgatatcca
     1381 acaggtccgg ctgtcccaaa cgcaacagca accccgccac cggcaggacc gcgatgggca
     1441 gcatcagcgc acgacccaga ggctggaggc tggagagacc gattttcatg ctgcggctcc
     1501 tgcggattgg cgcggcgttc ggtttcgcat cagggcttcg tttgcaacac gccggacagc
     1561 ctctgcgctg tcgagcttga gagcggtggc ggccagagcc tggcattcac cgagggtgaa
     1621 tccgcgcacg gtcgccttga tctcggcgac cacggccgga gcggcggaga gctcggtgac
     1681 ccccagtccg accagtatgg gggccgccag ccgatcggac gccagcccgc cgcagactcc
     1741 gatccattgc aggtccgctg ctcccgccgc cgaagctgcg atcaacctca gcaccgcagg
     1801 atggagcccg tccagttgca ccgccaaatg cgggttctgg cggtccatag ccagcgtata
     1861 ttgggtcagg tcgttggtgc cgatcgagac gaaatcgatc tcggcggcaa gcaggtcggt
     1921 ggtcattgcg gcggccgggg tctcgatcat gacgcccagc agaggcgccg ggcgacccgc
     1981 gtcggcgaca gcccggtcca gcagagcccg ggcctgccgc acctctgcga ccgaggcaat
     2041 catcggcaac atcaacgcca ccagaccgga cgactggacg cggcagacgg cgcgcatctg
     2101 agtgagcaga agctcgggat gcagcagtcc cgaccggacg ccccgcagcc ccagagccgg
     2161 attgtcttca tgcggcatcg gcagatacgg cagcggcttg tcgccgcctg catccagcgt
     2221 ccgtacaacc aaaggcctgc cgctgaggcc gtcggcgatc gcctgatact gggacaactg
     2281 ctcggcttca tccggcgcgg tctggcgatc gaggaacagg aactcggtcc gaagcaggcc
     2341 gcagccctcc gctccaccca ccaccgcccc gaccgtttcc gaggccgcgc cgaggttggc
     2401 gaacacctcg atccggacgc cgtcagcggt tcgacacggc tcggccgcga acgcgcgcgc
     2461 g
//
LOCUS       DASVTM010000163         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_283403,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000163 DASVTM010000000
VERSION     DASVTM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283403"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VF633_04545"
     CDS             complement(<1..290)
                     /locus_tag="VF633_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470358.1"
                     /translation="MRTLTLAGWLMVAVGVLCVLSLGYCAYDGNRDRRAAERLASANA
                     ETFGVTVAARDDLSAQTASDTKANSTLERDLTNAVSPLPDARPSDRRIALQC"
     gene            complement(290..817)
                     /locus_tag="VF633_04550"
     CDS             complement(290..817)
                     /locus_tag="VF633_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HEX8470359.1"
                     /translation="MAASPVAPSWLTLARTFLGAREVVGAKHSPLIMSWIQEIGAKTL
                     GMKVNDDETPWCGTFMALLMKRSGLSAPLIAVRAASWGRVGQWGRELIAPRLGCILVF
                     TRDGGGHVGLYVGEDATHFHVLGGNQSNSVSVSRIAKDRLAVGGMRWPKGPELPPAQV
                     VKLTAAGAPVSVSEA"
     gene            complement(959..2632)
                     /locus_tag="VF633_04555"
     CDS             complement(959..2632)
                     /locus_tag="VF633_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470360.1"
                     /translation="MSFGARIAFSSDGLGAPGGGVGHGPNNFPQSAILALGGRLRPCA
                     APYMGQSGTRIQTASGSQAAWMPAARSAMLRQRPNLWGNFSRGHNDGIMNSNPVGNPN
                     YTDWEEETLADYQAFASYAASNDYFVVFGTIPSDVAGETTWRSAVWAAQAAYVATLTA
                     IDPRVRFCPLTNLTPPSDYSIDTSSANTHSDERMVKAVMPNFLALMADVLPIETTDQV
                     IAAILAGTYPGMGTANLDTLSAMATTGGVVSGTGLSIGGDGFLPGGKTVTMTMVGATA
                     VASRIAKGDGSYKVRVTFDGTLTTAGKIMFGDTSNQARTASPGRYFRHGLRVKTTKGG
                     FRNFGADWGTQGSFAGTASSLATNALSGDNADPIADAIMWILPTATYGSDTSYTGSRR
                     WAFWWPAGTVFNAATDYVEFDCPFTFEGSDRGAVPAAYIGGTIGTGGTPVVFFGNNYR
                     ARLSGTYSATTGGTIRLETGMVQPEGLTNADFTRRAIYKGTSSDTAAGTGTLMHTYAS
                     AAVWSAALAGGLATAGQILFAEYDITDPTSGVVTTVRSRVTTDSLTVAA"
     gene            complement(2712..>3361)
                     /locus_tag="VF633_04560"
     CDS             complement(2712..>3361)
                     /locus_tag="VF633_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470361.1"
                     /translation="HLMTLSITAPGSANLKSNLDGGDHVVVHSEDTTQRAALLAALAA
                     LDPDEGLAAVVTALGGTLAISASALPLPAGAATQTTLAALLAKVIAAPATEAKQDAGN
                     TALGLLATQSTLAAVLAKMIAAPATEAKQDTLKASVDALFNAIKPATGHAAVTPGTAL
                     ATPSRALYIGGAGNISVKVGGVDATYAVTAGQTLNVVATDVNSAGTTATGIVSQF"
BASE COUNT          578 a         1069 c         1128 g          586 t
ORIGIN      
        1 tcacactgca gagcgatccg gcgatcagaa ggccgagcat cggggagcgg tgagacagca
       61 ttggtcagat ccctttcgag ggtggagttg gctttggtgt cggaggcggt ctgggcgcta
      121 aggtcgtccc ttgcggcgac cgtcacccca aatgtctcgg cattggcgct ggcgaggcgc
      181 tcagcagccc tgcggtccct gttgccgtca taggcgcagt aaccgagcga caggacgcac
      241 aggacgccga cggcgaccat tagccagccg gcgagggtca gggtgcgcat caggcttcgc
      301 tcaccgatac cggggcgccg gctgcagtca gtttcacgac ctgggcgggc ggaagctccg
      361 ggcctttcgg ccatcgcatc ccgcccaccg ccagccgatc cttggcgatc cggctcacgc
      421 tgacgctgtt cgactggttg ccgccgagaa cgtggaagtg ggtcgcgtct tcgccaacgt
      481 agagcccgac gtggccgccg ccgtcgcgcg tgaagacgag gatgcagccg aggcgcggag
      541 cgatcagctc gcgaccccac tggccaacgc ggccccatga cgcagcgcgc acggcgatca
      601 gcggcgcgga gaggcccgaa cgcttcatca acagggccat gaaggtcccg caccacggcg
      661 tctcgtcatc attgaccttc atgccgagtg tctttgcgcc gatctcctgg atccagctca
      721 tgatgagcgg gctgtgtttt gccccgacga cctctcgggc gccgaggaag gttcgggcaa
      781 gcgtcagcca cgagggcgcg acgggcgaag cggccatggt ctctctccag attgtcgatt
      841 tgatttagct gtgcgttcag cagcgcaggg gtcggtccga ccgaacggct cttaggcgac
      901 ccgcctgcgg acaaagcgat gcgagctggg tcgccgcacc ttcagagtgg cgtgaggcct
      961 aggctgcgac cgtcaggctg tccgtggtca ctctcgagcg aacggtcgtc accacgcctg
     1021 atgtgggatc ggtgatgtcg tattcggcga agagtatctg ccctgccgtc gccaagcctc
     1081 ccgccagagc cgcagaccag accgctgccg aggcataggt gtgcatcagc gtgccggtgc
     1141 ccgccgccgt gtcggacgaa gtgcccttgt agatggcgcg ccgcgtgaag tcggcattgg
     1201 tcaggccttc cggctgaacc attccggttt ccagccggat cgtgcctccg gtcgttgccg
     1261 agtaggtgcc ggacaggcgg gcgcgatagt tgttgccgaa gaagacgaca ggcgtcccgc
     1321 cggtgccgat cgtaccgccg atatacgcag caggcacagc gccccgatcc gacccctcga
     1381 atgtgaaggg gcaatcaaac tcgacatagt cagtcgccgc gttgaagacc gtgcccgccg
     1441 gccaccagaa ggcccagcgt cgtgacccgg tgtaggacgt gtcgctgccg taggtggcag
     1501 tcggcaaaat ccacatgatc gcgtcggcga tggggtcggc attgtcgccg ctcaacgcgt
     1561 tcgttgccaa tgaagaggcc gtgccggcaa atgaaccctg cgtaccccaa tcggcgccga
     1621 agttgcggaa gccgcccttc gttgtcttca cccgcagccc atgccggaag taccgaccgg
     1681 ggctcgcggt gcgcgcctgg ttcgaggtat cgccaaacat aatcttgccg gctgtcgtca
     1741 gcgtgccatc gaaggtcacg cggaccttgt aagatccgtc ccctttggcg atgcgcgatg
     1801 caactgcggt cgcgcccacc atcgtcatgg tcacggtctt gccgccgggc aggaatccat
     1861 cgccgccgat ggaaagcccg gttccggaca ccacgccacc cgtcgtcgcc atggctgaca
     1921 gcgtgtcgag gttggcggtt cccatgcccg gataggttcc ggcaaggatc gcggcgatga
     1981 cctgatctgt ggtctcgatc ggcagcacgt cggccatcag cgccaagaag ttcggcatca
     2041 ctgccttgac catgcgctca tcgctgtgcg tgttggcaga gctggtatcg atggaatagt
     2101 cggacggagg tgtaaggttc gtcagagggc agaaccgcac cctcggatca atagccgtca
     2161 gggtcgcgac ataggccgct tgagccgccc aaacggcaga tcgccacgtc gtttcgcccg
     2221 cgacatcaga cgggatcgtg ccgaacacga cgaaataatc gttggacgcg gcgtaggacg
     2281 cgaacgcctg atagtcggcc agcgtctcct cttcccaatc ggtgtagttg gggttgccga
     2341 ccgggttcga gttcatgatg ccgtcgttgt gaccgcgcga gaagttcccc cacaggtttg
     2401 gcctctgccg gagcatcgcc gagcgcgcgg ctggcatcca tgcggcctgt gacccgcttg
     2461 ccgtctggat ccgggttcca gactgcccca tgtagggggc tgcgcaaggc ctgagcctgc
     2521 cgcccaacgc aaggatcgcc gactggggga agttgttggg gccgtggcca acgccgccgc
     2581 ccggagcgcc gagaccgtcg ctgctgaacg cgatgcgggc gccgaacgac attcgctgcg
     2641 agccaatggg aagggcccca cctttcgttc gtgaccgcgc gagaccgagt ccctgaccga
     2701 ggcgcgccat cctagaactg gctcacgatg ccggttgcgg tcgtgccggc cgagttaaca
     2761 tcagtcgcga ccacattgag cgtttgcccc gcagtcactg cataggtggc gtcgacgcca
     2821 cctaccttga cgctgatgtt acccgccccg ccaatgtaga gtgcgcggga cggcgtcgcg
     2881 agagctgtgc ccggcgtcac cgcggcatgg cctgtcgccg gtttgatcgc gttgaacagc
     2941 gcgtcgacag acgccttgag cgtatcctgc tttgcctcag tcgcgggcgc cgcaatcatc
     3001 ttggccagga ccgccgcgag ggtggactgc gtagccagca agccgagagc ggtgttcccg
     3061 gcgtcctgtt tggcttccgt ggcgggagcg gcgatgacct tggccaacag ggccgcaagg
     3121 gtcgtctgcg tggcggctcc ggcagggagg ggaagggcag aggccgatat ggcaagggtg
     3181 ccgcccagag ccgtaacgac ggcagccaag ccctcgtcag ggtcgagagc cgccagagca
     3241 gccaacagcg ccgcgcgttg cgtggtgtcc tccgaatgaa ctacgacgtg atcgccgccg
     3301 tctaggttgg acttgaggtt cgccgagccc ggcgcggtga tcgaaagggt catcagatgg
     3361 c
//
LOCUS       DASVTM010000164         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_816006,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000164 DASVTM010000000
VERSION     DASVTM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816006"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VF633_04565"
     CDS             <1..1268
                     /locus_tag="VF633_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX8470362.1"
                     /translation="STGARLLVDTAIVPGGRSDFLTVGTTSGVTSVAIRDTATTSFGA
                     YNPTGIVIVNGVSHAGDFVLDSGSSFYNAGVFGGAIDKPGLFFSQLGVNGAGQTVLVS
                     LPKVQAYQFSTLASQAQAVWYETAPRAARQAEVRDQLASGDGAGGFWVDVQGTHTARD
                     VDRKSPTLAGIKSFDASYGQDVTAATIGYDAVRPMMNGDVVFGASVGYVNSSVDFDKQ
                     STSVDMDGVSGAAYASFVRSGWFVAGTVGVTQLNAEIKAPRLTGFTEQETDITSIGAT
                     LEAGFRAPFIMGTTIEPTAALAYVNTSVDDFTAAGSTFRFEDGESLRPSIGARVSGDT
                     SIGAGWATRYNVSVRAVGEALSENAITIGSAGPNLRVFDQFENAYGEVKAGLTSESVN
                     GWSVFGDITGRYSDEIRAVGASVGMRLRY"
     gene            complement(1395..2270)
                     /locus_tag="VF633_04570"
     CDS             complement(1395..2270)
                     /locus_tag="VF633_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX8470363.1"
                     /translation="MIAVLDVAALSACPLPRLPVDGDKEDRGSLFVMGGETVMAGAAL
                     LAGVAALRVGAGKLQLAATSDAVAAMSVAVPEARIVRKGAAGGLGKKVRAAARKASAL
                     VIGPGMDARPRQRRMALNLLREAPFSPALIDAGALPEAKEASVFAALSNGRVILTPHA
                     GEMAGMLGRDKAAVKADPLATAREASAMFRSVVVMKGVETFVVSPDGLAWQFKGGVAG
                     LGTSGSGDVLAGVIGGLLARGASPVTAALWGVVLHAEAGAALSEHAPLGFLARDLLEP
                     LPGLLGRLGEASAAA"
     gene            complement(2267..3019)
                     /locus_tag="VF633_04575"
     CDS             complement(2267..3019)
                     /locus_tag="VF633_04575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8470364.1"
                     /translation="MTSAWPARLWIVRHGQSAGNVARDRAHAEGLGEIDLDIRDVDVP
                     LSDLGHEQSAALGKWFAALPTEDRPTVVLTSTYLRARQTAAAIAEVGGVIGGDAPPSV
                     DERLREREFGILDRLTTQGIMARYPEQAEARRRLGKFYHRPPGGESWCDVILRLRAAL
                     DTLSLHHRDENVLIVGHQVVVLCLRYLLEGLTEDEILAIDAQGDVANCGVTTYRRSAR
                     CGLVLEGYNFVAPLQMEGAPVTTAPDVPVAPR"
     gene            3429..3800
                     /locus_tag="VF633_04580"
     CDS             3429..3800
                     /locus_tag="VF633_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470365.1"
                     /translation="MIGSTGASSASGVLEIPGNASTGDEFPSVSMSHDAGACRRGLRE
                     ISEIAAVASLENSQMSAQEALSTIAAIAEWVDEEEDGARADCSDIIGRLNDLTGSVDF
                     EVLDDRAAFDMFRSVLGVLRR"
     gene            complement(3861..4025)
                     /locus_tag="VF633_04585"
     CDS             complement(3861..4025)
                     /locus_tag="VF633_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470366.1"
                     /translation="MSNMAWVWGVVIIGGPLILAGAIAWAKLHSVPKTRKDDPDTPAD
                     DPSKGMPGHD"
     gene            complement(4025..4207)
                     /locus_tag="VF633_04590"
     CDS             complement(4025..4207)
                     /locus_tag="VF633_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470367.1"
                     /translation="MYDKTTNGCRDGQLNSLSEDSGGGDRFKKPGVGGQVLRNRVVRL
                     LDNRANDVERELAGAR"
BASE COUNT          804 a         1552 c         1533 g          818 t
ORIGIN      
        1 gttcgaccgg cgcgcgcctg ctggtcgata ccgccatcgt cccgggtggg cgttcggatt
       61 tcctgactgt cgggacgact tcgggcgtca cttcagtcgc gatccgcgat acggccacga
      121 ccagcttcgg cgcctacaac ccgaccggta tcgtgatcgt caacggcgtc agccatgccg
      181 gtgacttcgt cctcgacagc ggctccagct tctacaacgc aggcgtcttc ggcggggcca
      241 tcgacaagcc gggcctgttc ttcagccaac tcggcgtcaa cggcgcgggc cagaccgttc
      301 tggtcagcct gcccaaggtt caggcctatc agttctcgac gctcgcctcg caagctcaag
      361 ccgtctggta tgaaacggcg ccacgcgcgg ctcgtcaggc cgaggtgcgc gatcaactgg
      421 catcgggtga cggcgccggc ggcttctggg tcgacgttca gggcacgcat accgcccgcg
      481 acgtggaccg caagtcgccg acgctagctg gcatcaagtc cttcgacgct tcgtacggtc
      541 aggacgtcac cgccgccacg atcggctatg acgcggttcg cccgatgatg aacggcgacg
      601 tcgtcttcgg cgcttcggtc ggctatgtga actccagcgt cgacttcgac aaacagtcga
      661 cctcggtgga catggacggc gtctccggcg ccgcctacgc ctcgttcgtc cgcagcggct
      721 ggttcgtcgc gggtacggtc ggcgtcaccc agctgaacgc cgagatcaag gctcctcgcc
      781 tgaccggctt caccgagcag gaaaccgaca tcacgtcgat cggcgcaacc cttgaggcgg
      841 gcttccgggc tccgttcatc atgggcacga ccatcgaacc aacggcggcc ctggcctacg
      901 tcaacacttc ggtggacgac ttcacggcgg cgggttcgac cttccgcttc gaggacggcg
      961 aaagcctgcg gccttcgatc ggcgcacgtg tctccggcga cacgagcatc ggcgctggct
     1021 gggccactcg ctacaacgtc tcggttcgcg ccgttggcga agccctgtcg gagaacgcaa
     1081 tcacgatcgg cagcgccggt ccgaacctgc gtgtcttcga ccagttcgaa aacgcctacg
     1141 gtgaggtcaa ggctggcctg accagcgaga gcgtcaacgg ctggagcgtg ttcggtgaca
     1201 tcacgggccg ctatagcgat gagatccgcg ctgtcggcgc ctcggtaggc atgcgtctgc
     1261 gctactaggt cggcgaatcg accgctgaaa acgccgcgtc tcgtcagaga cgcggcgttt
     1321 ttcgcttcag tgacggtcgc accgaaagcc ggacatcggc cggcgaagca ggcttgaccg
     1381 ggtggctcca gccatcatgc ggcggctgaa gcttcgccaa ggcggccaag caggccaggg
     1441 agaggctcga gaagatcgcg ggcgagaaag ccgagaggcg cgtgttccga aagggcggcc
     1501 ccggcctcgg catggaggac aacgccccag agtgcggcag tgaccggcga agccccgcgc
     1561 gccagcaagc cgccaattac gcccgccagc acgtcgccgg aacctgaggt tcccagtccg
     1621 gcgacccccc ccttgaactg ccaggcgaga ccgtcgggcg acacgacgaa agtctctact
     1681 cccttcatga ccaccacgga tcgaaacatt gcggaggctt cgcgggctgt agccagcggg
     1741 tccgccttta cggctgcttt gtcgcgcccc agcatgccgg ccatctcccc ggcgtgcggg
     1801 gtcaggatga cccgaccgtt cgacagagcc gcgaatacag acgcctcctt cgcctcaggc
     1861 aaggcgccgg cgtcgatcaa ggcaggtgag aatggcgctt cccggagaag gttcaatgcc
     1921 atccgtcgct ggcgaggacg cgcgtccatc cccggtccga tcaccagggc ggaggctttt
     1981 ctcgccgccg cgcgcacctt cttgcccaag cccccggcgg cgcctttccg cacgatccgg
     2041 gcctcgggca ctgcaactga catggccgcg accgcgtccg acgttgcggc aagttgcagt
     2101 ttgcccgcgc ccactcgcaa tgcggcaacg cctgcgagca aggcggcgcc tgccatgacg
     2161 gtctccccgc ccatgacgaa cagggagcca cggtcttcct tgtccccgtc gacgggaagc
     2221 cggggaaggg gacaggcgga cagggcggcg acgtcgagga cggcgatcat cgcggcgcaa
     2281 ccgggacgtc gggggcggtc gtcaccgggg cgccctccat ctgcagcggg gcgacaaagt
     2341 tatagccctc cagcaccaat ccgcaccggg cgctgcggcg gtaggtggtg acgccgcagt
     2401 tggccacatc gccctgcgcg tcgatggcca ggatctcatc ctccgtcagc ccctcaagga
     2461 ggtaacgaag gcacaggacc accacctgat ggccgacgat caaaacgttt tcgtcgcggt
     2521 ggtgcaggct gagggtgtca agggccgccc gcaaacgcag aatgacatcg caccagctct
     2581 caccaccggg cggtcgatga tagaatttgc cgagccggcg tctcgcctcc gcctgctcgg
     2641 gatatcgcgc cattatgccc tgggtggtca gccggtcgag gatgccgaac tcgcgctcac
     2701 gcaagcgttc gtcgacggac ggcggcgcgt cgccgccaat aacacctcca acttcggcga
     2761 tggcggctgc ggtctgtcgg gcgcgtaaat aggttgaggt cagaacgacc gtgggtctgt
     2821 cttcggtcgg gagcgctgca aaccatttgc caagtgcggc ggactgctcg tgaccgagat
     2881 cgctaagcgg cacatcaacg tcgcgaatgt cgagatctat ctcgccgagg ccctccgcgt
     2941 gcgccctgtc tcgcgccacg tttcctgcgc tttgtccatg gcggacgatc cagagacggg
     3001 cgggccatgc cgaggtcatg atgggcgctt tcttcaccga ggagagacgc tcaacgcacg
     3061 agcgccgcgt tggagccctt cactcgaccg ccgggcgtgg gggttgtcgg caggcgggtc
     3121 ggaactctcc accgctcgcc aggttagagg atcatgacca aactcttgca ggacaaggac
     3181 gaccagtggc gggagaggcc aaacggcgcg acgcccgtat ttgacccggg gctatcagct
     3241 ctgggaaccg acgatgaggc tggcggagcg agagcacctg cggcgtcgcc ggggcctgga
     3301 agcgccgcac cgatgcccgc atcccccgtt tccgagcccc atggccccag gctgagaccg
     3361 tacgtctggt ggacagcggg ggctttgatc atcgtcgctc tggccctcac agcgatactg
     3421 agcttctgat gatcggcagc acgggtgctt cttcagccag cggcgtgctc gagatccctg
     3481 ggaatgcgtc gaccggagac gagtttccca gcgtttcgat gtctcacgat gcgggggcat
     3541 gtcgccgcgg gctccgcgag atcagcgaaa tcgcggctgt cgcctcgctc gaaaatagcc
     3601 agatgtcggc ccaggaagcg ctgtcgacaa ttgctgccat cgccgaatgg gtggatgagg
     3661 aagaggacgg cgcgcgcgca gattgctcgg acatcatcgg gcgtctgaat gatctgacgg
     3721 gctcggtgga cttcgaggtc ctggacgacc gcgcggcctt tgacatgttc agaagcgtcc
     3781 tcggggtgct gcgacgctga acggcgatcg gctttcagtc cagtacgcaa accttgcttc
     3841 gtacgggcct ttgcgcgcgc tcagtcgtgg cccggcatcc ctttggatgg atcgtctgcg
     3901 ggcgtgtcgg gatcatcctt ccgggttttc gggacgctat gcagtttggc ccaggcgatc
     3961 gcgccggcca gaataagggg gcctccgata atcaccacgc cccagaccca tgccatgttg
     4021 ctcatcagcg cgctcccgcc agttcgcgct cgacatcgtt ggcgcggttg tcgaggaggc
     4081 ggaccacgcg gttgcgaaga acctgtccgc cgacgccggg ttttttgaac cggtccccac
     4141 ctccggagtc ttccgaaaga ctgttcaact ggccgtcccg gcagccgttc gtggtcttgt
     4201 cgtacatatc gaacccttcg ctcggagcga ggccacccgc tcggtgacag ttgtgatgcc
     4261 tggcggaccg aagcgacgcc gttcccgtcg agcgctggtc gccacaagta atccgtcgtg
     4321 cgtcaagctg ttcctgtgtc gggacccgga cgggtcggtg ctttcggacg cccctcgctg
     4381 atgtcggacc gcgcgcgttg gcgatgccag ccgtcgggtg ggcgacatag caacctcggt
     4441 gcgcaaaagg ctttcactct cgcagagggg ggccggtcaa cgagagtcga cctccgttaa
     4501 tcaaggcgtc gggcatcagt ccgggcgtca atccaggaca actcttaggg agaccgccat
     4561 gctggcgatg gattatcggg ggccccggcg ggtccgaatt gcgcaaaagc ccatgcctgc
     4621 acttcagcat ccgcgtgacg ccatcgtccg cgtcacgcgg tcgtgcatct gtggctcgga
     4681 tctccacctc taccacggca tggtgcc
//
LOCUS       DASVTM010000165         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_763029,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000165 DASVTM010000000
VERSION     DASVTM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763029"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VF633_04595"
     CDS             complement(<1..531)
                     /locus_tag="VF633_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HEX8470368.1"
                     /translation="MSRRLLAASAAVLAFAAAPFAASAQDAATIRTAEQLRDKAMQSN
                     VAMDYVTQLTTRFGPRPAGSHSEQAAAAWAADYMKELGFKNVHIQQFPLVGWERGAES
                     GSIVGSNPQPLIVAALGHSPGTPEGGVEAEVVRFTSLEALQAAPDGSLTGKIALVDAG
                     QMHAMQDGSAYGPLTRI"
     gene            complement(626..976)
                     /locus_tag="VF633_04600"
     CDS             complement(626..976)
                     /locus_tag="VF633_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8470369.1"
                     /translation="MLRFIVQFLVTAAGLWLASVFVPGVAFTDTPSLLLAAVLLGIVN
                     AIVRPILVVLTFPITVVTLGLFLLVVNAATIGLVAVLLNGFVVNGLGAGIGAAIVTGV
                     ISWLAGAFIGDRKG"
     gene            1152..1457
                     /locus_tag="VF633_04605"
     CDS             1152..1457
                     /locus_tag="VF633_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX8470370.1"
                     /translation="MTDQRINGGVANLGGQVQEGLGNLTGDSKLQADGKLNQVKGKYL
                     DGYGKVIDRLDGFAGKAPTDLQEPARIGLEFARKKPFLTTAIVAGLGLLLAGAGRRR"
     gene            complement(1552..>2624)
                     /locus_tag="VF633_04610"
     CDS             complement(1552..>2624)
                     /locus_tag="VF633_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8470371.1"
                     /translation="ASQMLLPENKRKKVIGQKEVEAVIAKMARIPPKSVSKSDTEGLR
                     ELERDLNRAVFGQDAAIEQVASAMKLARAGLRDPQKPIGSFLFAGPTGVGKTEVAKQL
                     ASTLGIEMLRFDMSEYMERHTVSRLIGAPPGYVGHDQGGLLTDAVDQHPHAVVLLDEI
                     EKAHPDVYNILLQVMDNGMLTDAIGKKVDFRNVILIMTTNAGAADNARASIGFGRGKV
                     EGEDDKAIQRLFAPEFRNRLDAIVGFKPLQPETIRLVVTKFILQLEAQLADRNITIEL
                     SDDAADWLAKNGFDELYGARPLGRVIQENIKKPLADDILFGRLTRGGHVKVALKDGKI
                     VFDIAAASGEAVKETVDEEPAV"
BASE COUNT          456 a          864 c          869 g          435 t
ORIGIN      
        1 aatgcgcgtc agcgggccat aggcggagcc gtcctgcatc gcgtgcatct gacccgcatc
       61 gacgagcgcg atcttgccgg tcagcgatcc atcgggcgcg gcctgcaggg cctcgaggct
      121 ggtgaagcgg acgacctccg cctcgacacc gccctcgggc gtgccgggcg aatggccgag
      181 cgccgcgacg atcagcggct gcgggttgga cccgacgatg gaaccgcttt cagccccacg
      241 ttcccagccg accagcggga actgctggat gtggacgttt ttgaagccaa gctctttcat
      301 gtagtcggcg gcccaggcgg cggcggcctg ttcggaatgc gacccggccg ggcgcggacc
      361 gaagcgcgtc gtcagctggg tgacgtagtc catggccacg ttggactgca tcgccttgtc
      421 gcgcagttgc tcggccgtgc ggatcgtggc ggcgtcctgc gcagaggccg cgaagggcgc
      481 agcggcgaag gcgaggacag cggcgctggc ggcgagcaga cggcgggaca tgggcgactc
      541 cggtcggacg aggaagaccg gaccctaaac gctcagccgc atggggcaag cgccgcagtc
      601 gtccattggc agtagtcagc cacggtcagc ccttgcggtc gccgatgaag gcgccggcca
      661 gccagctgat cacgccggtg acgatcgccg ctccgatccc ggcccccaga ccattgacga
      721 cgaagccgtt aagcagcacc gccaccagcc cgatggtcgc cgcattcacg accagcagga
      781 acaggccgag cgtgacgacc gtgatcggga aggtcagcac cacgaggatc gggcgcacga
      841 tggcgttgac gatcccgagc aggacggcgg ccagcaacag cgacggcgtg tcggtgaacg
      901 ccacccccgg cacgaacacg ctggccaacc acagacccgc ggcggtgaca aggaattgga
      961 cgatgaaacg cagcatggcg gaccctgtct gaaacggcgc cgccactgtg gcgcatttcg
     1021 ggtgcggcgt cttgcccgga gggagccgca gacgtgggtc gcgcggaacc ttggccgggc
     1081 ctgtgcacta agcgagcaga ctgttcgagc gtctcccctg ctcgaagttt ttcagcgcaa
     1141 aaggacctca catgaccgac caacgcatca acggcggcgt cgccaacctc ggcggccaag
     1201 tgcaagaagg cctcggcaac ctgaccggcg attcgaagct gcaagccgac ggcaagctga
     1261 accaggtcaa aggcaaatac ctcgacggct acggcaaggt catcgaccgg ctggacggct
     1321 tcgcgggtaa ggccccgacc gacctgcagg agcctgctcg catcggtctt gagttcgccc
     1381 gcaagaagcc attcctgacc acggccatcg tcgccggtct cggcctgctg ctcgccggcg
     1441 ccggtcgccg ccgctagtct cttcgcgaac ataaaagaaa ggcccggacg tcaccgtccg
     1501 ggccttttgc gttttagggt cggccctccc ccaccggggg gaggatctgg atcagaccgc
     1561 aggttcctcg tcgacggttt ccttgaccgc ttcgccgctc gccgcagcga tgtcgaagac
     1621 gatctttccg tccttgaggg cgaccttgac gtgaccgcca cgcgtcagac gtccgaacag
     1681 gatgtcgtcg gccagcggct tcttgatgtt ctcctgaatg acccggccca gagggcgcgc
     1741 gccgtacagt tcgtcgaagc cgttcttggc cagccagtcg gccgcgtcgt ccgagagctc
     1801 gatggtgatg ttgcgatccg ccagctgggc ttccagctgc aggatgaact tggtcaccac
     1861 caaacggatg gtctcgggtt gcagcggctt gaagccgacg atggcgtcga ggcggttgcg
     1921 gaactccggc gcgaacaggc gctggatagc cttgtcgtcc tcgccctcga ccttgccacg
     1981 accaaagccg atcgaggccc tggcgttgtc ggcggcgccc gcgttcgtgg tcatgatcag
     2041 gatcacgttg cggaagtcga ccttcttgcc gatcgcgtca gtcagcatcc cgttgtccat
     2101 cacctgaagc aggatgttgt agacgtcggg gtgagccttc tcgatctcgt ccagcaggac
     2161 cacggcgtgc ggatgctgat cgacggcgtc ggtcagcagg ccgccctgat catgcccgac
     2221 atagcccgga ggcgcgccga tcaggcggct gacggtgtgc ctctccatgt attccgacat
     2281 gtcgaagcgc agcatctcga tgccgagcgt ggacgccagt tgcttggcga cctcggtctt
     2341 gccgacgccg gtcggaccgg cgaacaggaa cgaaccgatc ggcttttgcg gatcacgcag
     2401 accggcgcgg gccagcttca tggccgaggc cacctgctcg atcgccgcgt cctgaccgaa
     2461 caccgcgcgg ttcagatcgc gctcgagttc gcgcaggcct tcggtgtcgg acttggagac
     2521 cgacttcggt ggaatgcgcg ccatcttggc gatgacggcc tccacttcct tctggccgat
     2581 gaccttcttg cgcttgttct cgggcagcag catctgcgac gccc
//
LOCUS       DASVTM010000166         3994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_602342,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000166 DASVTM010000000
VERSION     DASVTM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602342"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF633_04615"
     CDS             <1..663
                     /locus_tag="VF633_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8470372.1"
                     /translation="AGGGFGGFGQEGGPGGRASFDNIDLDELFGRFGGGAGARPGTRS
                     PFNGRGQDVRATLDISLEDSITGATRRIQFSDGRTLDVAIPKGAGDGQTIRLKGQGAP
                     GRGGATGDALIELKISAHPLFKIEGADLTMDQPVSLYDAVLGGKIPVRTPEGTVSMTI
                     PKGSQSGKVLRLKGRGAFANGKRGDLLAKLMITLPDDEDDALTKLAQKRRDEAPARPF
                     RD"
     gene            666..1826
                     /locus_tag="VF633_04620"
     CDS             666..1826
                     /locus_tag="VF633_04620"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX8470373.1"
                     /translation="MNKPDIKPARPWFSAGPTAKRPGYSLAGLPQELLGRGIRAPEVV
                     ERFAHGLRLTREVLEVPESHVMLYTPGSDTGAVEAALWGMLGERPVQVVAYENFGLTW
                     LADVKEHLGLEPEALTAPWGELPDMSKADWSRDVVFPWNGTTSGVRVPDAEWIADDRE
                     GLAICDATSAAFAMPLPWDKLDVVTFSFQKALGGEAGIGVVVLSPRAVARLDSYKPER
                     AIPKVLRLTNGKGFDRALADGVAINTFSVLTIEDWIDALGWARDVGGLTELIRRTYAN
                     YSALETWVEATDWIAFLPDRPEIRSTTSVCLKFTDARIEALDAKAQKAFVARFKALLE
                     GEAAVFDMEPHRNAPPGLRLWCGCTVETADVVAATPWLEWAFQTAVGELAAA"
     gene            1911..3206
                     /locus_tag="VF633_04625"
     CDS             1911..3206
                     /locus_tag="VF633_04625"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX8470374.1"
                     /translation="MANVAVVGAQWGDEGKGKIVDWLSNRADIVVRFQGGHNAGHTLV
                     VGGKVYKLALLPSGVVQGKPSIIGNGVVVDPWHLVSEIEKIEAQGVTINPDILTIADN
                     ACLILPIHPALDVAREAAASAPGAKIGTTGRGIGPAYEDKVGRRAIRVCDLASEEDLK
                     VKIERLRSHHDPLRAGLGLEPIDSNALLAQLLEIAPRILPYAQPAWRVLDQAQKAGKR
                     VLFEGAQGAFLDVDHGTYPYVTSSNTVAGQAAAGSGLGPKGVGYVLGIVKAYTTRVGE
                     GPFACELFDDVGKHLATIGREVGVNTGRARRCGWFDAVLVRQSVAINGIDGIALTKLD
                     VLDGLETLKICVGYKIDGEILDYLPSSLKAQAAAEPVFEELAGWRESTVSARSFKDLN
                     ANAIKYVRRIEELIGAPVAILSTSPERDDTILVRDPFLG"
     gene            3294..>3994
                     /locus_tag="VF633_04630"
     CDS             3294..>3994
                     /locus_tag="VF633_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470375.1"
                     /translation="MFAADARTLSRIAPTIQRVVIVDPNLAAARLLVDIMKSLGCREV
                     IHEPDAARALDAVREIEPGLIFTERLGPRLDGEKLTRTLRRSNLACRRAPIIMVTADA
                     TASTIKGARDCGVHEFLRKPFTSADLFRRVENVALKPRDWIEGVAYVGPDRRRFNSGE
                     YAGPQKRKQDKPASAAAAAMAVKDQAMRILAAALAQFDSDPLQAVRAARQQAETLKGL
                     AIKTSDAKLAIAAAGL"
BASE COUNT          716 a         1176 c         1375 g          727 t
ORIGIN      
        1 gctggcggcg gctttggcgg ttttggccag gaaggcggcc ccggcggtcg cgccagcttc
       61 gacaacatcg acctcgacga gttgttcggt cgtttcggcg gcggcgctgg agcgagaccc
      121 gggacgcggt cgccgtttaa cggtcgcggt caggatgttc gggcgacgct cgatatcagt
      181 cttgaagact cgatcacggg ggcgacccgc cgcatccagt tttcggacgg tcggacgctg
      241 gatgttgcta tcccgaaggg cgccggagat ggccagacca tacgtctgaa agggcagggt
      301 gcgcccggtc gtggcggcgc gacgggtgat gcgctgatcg agctgaagat atccgcacat
      361 ccgctgttca agatcgaggg cgcggacctg accatggatc agccggtgtc gctttatgat
      421 gcggtgctgg gcggcaagat tccggtgagg acgcccgagg gtaccgtcag catgacgatc
      481 ccgaagggct cgcaatcagg caaggttctg cggctcaagg ggcgcggcgc attcgccaac
      541 gggaagcgtg gcgatctgct ggccaagctc atgatcactt tgccggacga cgaggacgac
      601 gcattgacca agctcgcgca gaaacgtcgc gatgaggcgc ctgcacggcc tttccgggac
      661 tgaaaatgaa caagcccgac atcaagcctg cgcggccatg gttttctgct ggtccgaccg
      721 cgaagcgccc gggctacagt ctggcgggac tgccgcaaga actgctagga cgcggtatcc
      781 gcgcgcctga ggtggtggag cggttcgcac acggcctgag gctcacgcgc gaggtgctcg
      841 aagtgccaga gagccacgtg atgctctaca cgccggggtc cgatacggga gcggtcgagg
      901 cggcgctgtg gggcatgctt ggcgaacggc cggtgcaggt cgtggcgtac gagaatttcg
      961 ggctgacatg gctggcggat gtgaaggagc atctgggtct ggagccggag gcgctgacgg
     1021 cgccgtgggg cgaactgccg gatatgtcca aggcggactg gtccagggat gtggtgttcc
     1081 cgtggaacgg cacgacctcg ggcgtgcgcg tgcctgatgc cgagtggatc gccgatgatc
     1141 gcgaagggtt ggcgatctgc gacgccacgt ccgcagcatt cgctatgccg ctgccctggg
     1201 acaagctgga tgtcgtgacc ttctcgttcc agaaggcgct gggcggtgag gcgggcatcg
     1261 gcgtggtggt gttgtcgcct cgcgcggtgg ccaggctgga tagctacaag cccgaacggg
     1321 ctattcccaa ggttctgcgt ctgaccaacg gcaagggctt cgatcgggcg ttggcggacg
     1381 gggtggcgat caacactttc tcggtcctga ccatcgagga ctggatcgat gcgttggggt
     1441 gggcgcggga tgtcggcggg ctaacggagc tgatccgccg gacgtacgcg aactattcgg
     1501 cattggaaac gtgggtcgag gcgaccgact ggatcgcttt cctgccggac aggccggaaa
     1561 tacgctcgac gacttcagtc tgtctgaagt tcaccgacgc gcggatcgag gcgctcgacg
     1621 ccaaggcgca gaaggctttc gtggcgcggt tcaaggcgtt gctggaaggc gaagcggcgg
     1681 tgttcgatat ggagccacat cgcaacgcgc cgccggggct gaggctctgg tgcggttgta
     1741 cggtcgagac tgccgacgtt gtggcggcca ctccatggct ggaatgggcg ttccagacgg
     1801 cggtcggcga attggccgct gcataaaaca tcccgaccgg gacgacattc gccgattccc
     1861 tcgctatagg ctgcgcccgc ccatacaggc atcggtgcgg agagacagtt ttggcgaatg
     1921 ttgcagtagt cggcgctcaa tggggcgacg agggcaaggg caagatcgtc gactggctgt
     1981 cgaaccgtgc cgacatcgtc gtgcggttcc aaggcggtca caacgccggc cacacgctgg
     2041 tcgtgggcgg caaggtctac aagctggccc tgcttcccag cggcgtggtt cagggcaagc
     2101 cctccatcat cggcaacggc gtagtcgtcg atccctggca tctggtcagc gagatcgaaa
     2161 agatcgaggc gcagggcgtc accatcaacc ccgacatcct gaccattgca gataatgcct
     2221 gcctgatcct gccgatccac cctgctctgg acgttgcccg cgaggctgcg gcaagcgcgc
     2281 cgggcgccaa gatcggcacc accggtcgcg ggatcggccc tgcctatgag gacaaggtgg
     2341 ggcggcgcgc catccgggtc tgcgacctgg ccagtgaaga agacctgaag gtcaagatcg
     2401 agcgcttgcg gtcgcatcat gatccattgc gggccggtct gggactggag ccgatcgatt
     2461 ccaacgcgct gttggcgcag ctcctggaaa tcgcgcccag gattctgccc tatgcccagc
     2521 cagcatggcg agttctggac caggcgcaga aggcgggcaa gcgggtgctg ttcgagggtg
     2581 cgcagggcgc cttcctcgac gtggaccatg gcacctatcc ctatgtcacc agctccaata
     2641 ccgtagctgg ccaagccgct gcggggtcgg gccttggtcc caagggggtc ggctatgtgc
     2701 ttgggatcgt gaaggcttat acgacccgcg tcggtgaagg ccccttcgca tgtgagctct
     2761 tcgatgatgt cggaaaacac ctcgcgacta tcgggcgcga ggtcggggtc aataccgggc
     2821 gcgcgcggcg ctgcggctgg ttcgacgccg tgctggtgcg tcagtctgtt gccatcaacg
     2881 gtatcgacgg catagcactg accaagctcg atgttctgga cggcctcgag acgctgaaga
     2941 tctgcgtcgg ctacaagatc gatggcgaga ttctcgacta tctgccgtcg agcctgaagg
     3001 cccaagcagc agctgagccg gtattcgagg agctggcagg ctggcgcgaa agcacggtgt
     3061 ccgcgcgtag cttcaaggac ctgaacgcca acgcaatcaa atatgttcgt cgaatcgagg
     3121 aactgatagg ggcgcccgtg gcgatcctgt cgaccagccc tgagcgtgac gacacaatcc
     3181 tcgttcggga cccattcctc ggttaggtat ggacctgttg gcgtagatgc ggctgttcaa
     3241 cgccgtctta accggacgct gttagggata atgctccatc ccggagagct cccttgtttg
     3301 ctgccgacgc ccgaaccctg agccgtatag cgccgaccat ccagcgtgtg gtcatcgtcg
     3361 atccgaatct ggctgctgct cgcttgctgg tcgacattat gaagagcctt gggtgccggg
     3421 aggtgattca cgagccggac gcggcacggg cgctggacgc agtccgcgag atcgagcccg
     3481 gcctcatctt taccgagcgg ttgggccccc gcctggacgg cgagaagctg acccgaaccc
     3541 tacgacgctc aaatctggct tgccgtcgcg ctcccatcat aatggtgacg gctgatgcca
     3601 cggcctccac aatcaagggc gctcgtgatt gcggcgtgca tgagttcctt cgaaagccgt
     3661 ttacctctgc cgatcttttc cgacgggtcg agaacgtcgc cctgaaaccg cgcgactgga
     3721 tcgagggcgt agcctacgtc ggtcctgacc gccgtcgctt caactctggc gaatacgcgg
     3781 gaccccagaa gcgcaagcag gacaaaccag cctccgccgc cgccgcggcg atggccgtga
     3841 aggatcaggc gatgcgaatc ctcgccgcag ctttggctca gttcgatagc gatccgctgc
     3901 aggcggtccg cgctgcacgg cagcaggctg aaaccttgaa aggcctggcc ataaagactt
     3961 ccgacgcgaa gctggctatc gcggcggcgg gtct
//
LOCUS       DASVTM010000167         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_71112, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000167 DASVTM010000000
VERSION     DASVTM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71112"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF633_04635"
     CDS             <1..265
                     /locus_tag="VF633_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX8470376.1"
                     /translation="AEGQNISTVTASGNVYYVTPTETIRGDRAVYTVSNATIIVTGDV
                     ILTQGKNVLTGGRLSYNIDTGQTNMVAGGNGRIQGVFYPQGAN"
     gene            301..1095
                     /gene="lptB"
                     /locus_tag="VF633_04640"
     CDS             301..1095
                     /gene="lptB"
                     /locus_tag="VF633_04640"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX8470377.1"
                     /translation="MARKELSALNVNDPAAAVAPTLVAESTKGLRVKNIARAFGARQV
                     VRDVSLTVQRGEVAGLLGPNGAGKTTCFYMITGLIPADTGSIWLDGEDITAQPMYQRA
                     RMGLGYLAQEASIFRGMTVEQNIKAVVELRESGRGIETETTRLLEELHIDHLRHAPAS
                     GLSGGERRRVEIARSLAGRPSFMLLDEPFAGIDPLAIADIRQVIRYLADEGIGVLITD
                     HNVRETLDIIDRASIMSNGEVLFEGTADQVIHDPEVRRVYLGDLYG"
     gene            1191..>2110
                     /locus_tag="VF633_04645"
     CDS             1191..>2110
                     /locus_tag="VF633_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX8470378.1"
                     /translation="MVGQRLEFRQGQGLVITPQLQQAIKLLQLSNQELDEYVETELEK
                     NPLLQRDETDAAPSRENGDDSFEVPQSSDGAELSFSDAGVSDRNSTVDAGDSDVYGDA
                     TPGERTVDRLSDDNAQPGLSDWSSAGKGGSSFSDGEGGERPDQRAITLWEHLQAQASS
                     AGFDATDHAIALSLIDAVDEGGYLRGELAEIADRLGCGLDRVEAVLKVCHGFEPTGIM
                     ARSVPECLTLQLVERNRFDPAMAALLDNLELLARRDMNGLRQACGVDGEDLIEMIAEL
                     KALTPRPGAGFGGETPQTVIPDVHVRPDPAG"
BASE COUNT          376 a          694 c          691 g          349 t
ORIGIN      
        1 cgccgaaggt caaaacatct cgaccgtcac cgcctcgggc aacgtctatt acgtcacccc
       61 gaccgagacg atccggggcg accgcgccgt ctacacggtg tcgaacgcga ccatcatcgt
      121 cactggcgac gtcattctga cgcaaggcaa gaacgtcctg accgggggcc gtctcagcta
      181 caacatcgat acgggccaga cgaacatggt cgcgggcggc aatggccgca tccagggcgt
      241 cttctatccg cagggtgcga actaggccct cgcggctccg gttcgactga aaggctccgt
      301 ttggcgcgca aggaactctc cgctctcaac gtcaacgacc cggccgctgc ggtcgcgccg
      361 acccttgtcg ctgagtccac caaaggtctg cgggtcaaga acattgctcg cgccttcggt
      421 gcacggcagg tggtgcgcga cgtctcgctg acggtccagc gcggcgaggt cgccgggctt
      481 ctgggaccga acggcgccgg caagacgacc tgtttctaca tgatcaccgg cctgatcccc
      541 gctgacaccg gctcgatctg gctggacggc gaggacatca cagcccagcc gatgtaccag
      601 cgcgcccgta tgggcctggg ctatctggcg caggaagcct cgatcttccg cggcatgacc
      661 gtcgagcaga acatcaaggc ggtggtcgaa ctgcgcgaga gcggccgggg gatcgagacc
      721 gagaccacgc gcctgctcga agaactccac atcgatcacc tgcgtcacgc cccggcctct
      781 ggcctgtcgg gcggcgagcg acgccgggtc gagatcgcgc gttcgctggc ggggcgtccg
      841 tccttcatgc tgctggatga gcccttcgcg gggatcgacc cgctggccat cgccgacatc
      901 cgtcaggtga tccgctatct ggcggacgag ggcatcggcg tgctgatcac cgatcacaac
      961 gtccgcgaga cgctggacat catcgaccgg gcttcgatca tgtcgaacgg cgaggtcctg
     1021 ttcgagggga ccgccgatca ggtgatccac gatcccgagg tccgccgcgt ttatctcggc
     1081 gacctgtacg gttgagacct gtcccgcgcg tcatcccgcg ttaactcctt gccttcattc
     1141 tgcccgacag caagttccgg gccaagtgcc cgatcgggag atgaaacggc gtggtcgggc
     1201 agaggctgga gttcagacag gggcaggggc tggtcatcac gccccagctg cagcaggcga
     1261 tcaagctgct gcaactgtcg aaccaggagc tggacgaata cgtcgagacc gaactcgaaa
     1321 agaacccgct gcttcagcgc gacgagaccg atgcagcgcc ctctcgcgag aatggcgacg
     1381 acagctttga agtcccgcag tcgagtgacg gcgctgaact gagcttctcc gacgcgggcg
     1441 tttcggatcg caactcgacc gtcgatgcgg gcgacagcga cgtctatggc gacgcgacgc
     1501 cgggcgaacg caccgtcgat cgcctgtctg acgacaacgc ccagcccggg ctgagtgact
     1561 ggtccagcgc cggcaagggc ggatcgtcct tctcggatgg cgaaggcggc gaacgcccgg
     1621 accagcgcgc catcaccctg tgggaacatc ttcaggccca ggcgtcgtca gccgggttcg
     1681 acgctacgga tcacgccatc gccctgagtc tgatcgacgc cgtcgatgag ggcggctatc
     1741 ttcgtggcga actggccgag atcgccgatc gtctcggctg cgggctggac cgcgtcgaag
     1801 cggtcctgaa agtctgtcac gggttcgagc cgaccgggat catggcgcgg tcggtgccgg
     1861 aatgcctgac gctgcagttg gtcgagcgca atcgcttcga tccggcgatg gcggccttgc
     1921 tcgacaatct ggagcttctg gcgcggcgcg acatgaacgg cctgcgtcag gcctgcggtg
     1981 tcgatggcga agatctgatc gagatgatcg ccgagctgaa ggcactgaca ccgcgtcccg
     2041 gcgcgggctt cggcggcgag acgccccaga ccgtcattcc cgatgtgcac gtccgacccg
     2101 atcccgccgg
//
LOCUS       DASVTM010000168         7356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_230769,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000168 DASVTM010000000
VERSION     DASVTM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230769"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VF633_04650"
     CDS             complement(<1..804)
                     /locus_tag="VF633_04650"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX8470379.1"
                     /translation="MNITVVGSINLDFVASAPSLPAPGETVTGATLMQHPGGKGANQA
                     LAAEKLGAEVALIGRVGNDGMADVALSLLEDFGVDLAGVEVDIAAPTGVALIAVDPSG
                     ENQIVVAAGANHMVTPEQLPARIEGPLIVQLELPIETVEAAVGRAVGFVCANLAPAAP
                     VSEALLRRADLIVVNEVEAAFYGDLLHHGGGRVVVTQGARGAEMYHRGVKMAWATPPT
                     ITAVDATGAGDAFVGAITVALLEGMDPADALRFACAAGAVAATRAGAQPS"
     gene            complement(801..1775)
                     /locus_tag="VF633_04655"
     CDS             complement(801..1775)
                     /locus_tag="VF633_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX8470380.1"
                     /translation="MLSAATLAKPNPMKTRALIIDCDPGVDDAVGLFLAFASPELELL
                     AITTVGGNVPVAATARNARIMRQIAGREDVPAYAGADRPLVRDPAGAGEFHGAEGLGD
                     LTAFEPNVGPAEGHAVDAIINLVMARPAGEVALAVMGPMTNLALAMRKEPPLAAHLGP
                     VVVMGGARTEGGNITASAEFNVWADPDAAAEVFASGCEMIVLGLDVTHQVRATEDRIA
                     AIEAIGSPAADATASMLRFSQRIEREIVRSDAPPVHDPCTVAWLVSPGLFETRPCRVE
                     VETVSDLTRGHTAVEFRVDPATARHHWATKADGQGVFDLITERLKAAK"
     gene            1857..3659
                     /locus_tag="VF633_04660"
     CDS             1857..3659
                     /locus_tag="VF633_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8470381.1"
                     /translation="MNSKVAAGLATLLLSTTTLTGSGAALAQTAPVLAATPVPNPALA
                     TLLTDYEAFLKTNDPISAGFEGDQAAKPKLPDVTRAAEVAQQAPLEAFLARAEALPST
                     GLSSDDRLNRDFLIWVLKRAIESIRYDSSRLAFTSEGGIGQNMAYVASSTRITSRSDA
                     DAWMARLVGLPKLYDDSLENARRGLATGFTQPKSVTESYLVSLRNEAAFTPETDPLLK
                     PFSVLPASIPAADQATLKARAAAIVAEQITPRRRQWLAFVQNDYLPAASATVGIGSRP
                     GGKEYYAFQARSYTTTDLTPDQIHQIGLDEVTRIRARMDVEMRASGWTGDFAGFLNHL
                     RTDPQFYATSREDMIEKASEMAKRADGGLPALFRTLPRLTYNVRPVPLDIEDTYTTGR
                     YNGGSLAEGVPGGYIVNTGKLDQRPLYELPALTLHEAVPGHHIQIALQQEAAQGPYFR
                     RNVDVTAFTEGWGLYSEFLGEEMGFYRTPEERFGRLSYEMWRACRLVADTGMHAMGWT
                     IEQARQCFTDNSALAPHNIETELQRYIGWPGQALAYKIGEIRLRAIRTRAETALGDKF
                     NVRDFHDALLVDGPLPLDLLDLRMDAWVAARKAE"
     gene            complement(3663..4217)
                     /locus_tag="VF633_04665"
     CDS             complement(3663..4217)
                     /locus_tag="VF633_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HEX8470382.1"
                     /translation="MNRKTQLIVLAGVIFLFFAAYFASPLLAVRGLISAAKVGDEAKL
                     EKLVDFPAFRTSLKEELNSKLVEQMRADLGDRADGLSGLGMIFAPTLISGAVDAFVTP
                     PAVAAMVRSGEVPNARDVARDRARAPNEPSTKVRHGYSYRGLNTFAVRLMRDDRPGDY
                     VDLLLERRNVFGWKLAAIDLPRKR"
     gene            complement(4270..4914)
                     /locus_tag="VF633_04670"
     CDS             complement(4270..4914)
                     /locus_tag="VF633_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HEX8470383.1"
                     /translation="MSHEPTTESDHRPGWAQAEARRAAARREPSFKRLILNLIVVGIV
                     LAAVSFFLAPAVAFIGIRSAAEAGDVAGLGKLIDYDAVRASLRPQVSGRVEALTPPPS
                     ILQDPIGAIRRQFEQATPQFVSTAPPVDAWLAPGPLLALTSGSGRDALTVGANPAVET
                     ARRWPRPTYWSVNRARLSVADDIHSPTVFTFERRGPYEWKLVHIGLPREGSTAP"
     gene            complement(5049..6269)
                     /locus_tag="VF633_04675"
     CDS             complement(5049..6269)
                     /locus_tag="VF633_04675"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX8470384.1"
                     /translation="MAKIKVANPIVDIDGDEMTRIIWQWIKDKLVFPFVDVDLDYYDL
                     GMEHRDATDDQVTIDAAHAIQKHGVGVKCATITPDEARVKEFGLKKMWKSPNGTIRNI
                     LGGVVFREPIICQNVPRLVPGWTQPIVVGRHAFGDQYKATDFLVPGPGKLTMKWVGAN
                     GEEQNYEVFDFPSAGIAMGMYNLDDSIRDFAHASFSYGLNRNFPVYLSTKNTILKAYD
                     GRFKDIFQEVFDAEYADKFKAAGLTYEHRLIDDMVAAAIKWSGGFVWACKNYDGDVQS
                     DVVAQGFGSLGLMTSVLMTPDGKVMETEAAHGTVTRHFRQHQKGEATSTNSIASIFAW
                     TAGFKHRAKLDDNAELAAFAETLEKIVVDTVEAGFMTKDLALLVGDQQGWLTTEGFLD
                     KVAENLRAELPNVG"
     gene            6471..6605
                     /locus_tag="VF633_04680"
     CDS             6471..6605
                     /locus_tag="VF633_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470385.1"
                     /translation="MDQETAALVQRWILTFLEQPPLIDAELMRHVLAEHDNRVAEPSS
                     "
     gene            6602..6814
                     /locus_tag="VF633_04685"
     CDS             6602..6814
                     /locus_tag="VF633_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEX8470386.1"
                     /translation="MTEKTFNPGDAVKWDHSQGTTTGKVILKVTKPMKIKDHKVAASS
                     ENPEYLVKSDKTGAEAAHKPSELRKA"
     gene            6837..>7356
                     /locus_tag="VF633_04690"
     CDS             6837..>7356
                     /locus_tag="VF633_04690"
                     /inference="COORDINATES: protein motif:HMM:NF018389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEX8470387.1"
                     /translation="MRKLWTGAAALALAACGGEQSSARPAEATQTPAATRAIASTARS
                     ESRTFRDWYAVCDNGNLCSAYSGGSTGWILVQMEAGPAAQPTVRVGMWPDNGEELKTP
                     ITLVIDGKRHATTPGPEDTSSAMLPAADARAVVAELTAGRAVNISSGDQTIELPVAGV
                     SASLLWFDERQGR"
BASE COUNT         1288 a         2348 c         2503 g         1217 t
ORIGIN      
        1 ggagggttga gcgccggcgc gggttgcggc cacggccccg gcggcgcagg cgaagcgaag
       61 ggcatcagcg ggatccatgc cctccagcag ggcgaccgtg atggccccga cgaaggcatc
      121 tccggccccg gtcgcatcga ccgccgtgat ggtcggcggc gtggcccagg ccatcttgac
      181 gccccggtgg tacatctcgg ccccacgcgc gccctgagtg acgaccaccc tgccgccgcc
      241 gtggtggagc aggtcgccat agaaggcggc ctcgacctcg ttgacgacga tcaggtcggc
      301 gcggcgcagc aaggcctcgg acaccggcgc ggcgggggcc aagttggcgc agacgaagcc
      361 gacggcgcga ccgacagcgg cctccaccgt ctcgatcggc agttcaagct ggacgatcaa
      421 cgggccttca atgcgggcgg gcagctgttc tggcgtgacc atatggttgg cccccgccgc
      481 gaccacgatc tggttctcac cggaggggtc taccgcgatc agggcgacgc cggtgggggc
      541 ggcgatgtcg acctcgacgc ccgccagatc cacgccgaaa tcttccagca gcgagagggc
      601 gacgtcggcc atgccgtcgt tgccgacgcg gccgatcagg gcgacctcgg caccgagctt
      661 ttcggccgcc agcgcctgat tggcgccctt gccgcccgga tgttgcatca gggtggcgcc
      721 ggtcaccgtc tcgccgggag cgggcaagct aggagcggag gcgacgaaat ccaggttgat
      781 agagccgacg acggtgatgt tcatttggcc gcctttagcc gctcggtgat caggtcgaac
      841 accccctgcc cgtcagcctt cgtcgcccag tgatgccgtg cggtcgccgg gtcgacacgg
      901 aactcgaccg ccgtgtggcc tcgggtgagg tcggatacag tctcgacctc gacgcgacag
      961 ggccgggttt caaacaggcc gggactcact agccaggcga ccgtgcaggg gtcgtggacg
     1021 ggcggcgcat cgcttctgac gatctcgcgt tcgatccgct gcgagaagcg gagcatggag
     1081 gccgtggcgt cggcggcggg cgagccgatg gcctcgatcg cggcgatacg gtcctcggtc
     1141 gcgcgcacct gatgggtgac gtcgagcccc agcacgatca tttcgcagcc gctggcgaag
     1201 acctcggctg cggcgtcggg gtcggcccag acgttaaact ccgcggaggc ggtgatgttg
     1261 ccgccctcgg tgcgtgcgcc acccatgacg accaccgggc cgaggtgggc tgcgaggggc
     1321 ggttccttgc gcatcgccag cgcgagattg gtcatcgggc ccatgaccgc gagagcgacc
     1381 tcgccggctg ggcgggccat gacgaggttg ataatggcat cgacggcgtg gccctctgcc
     1441 ggtccaacgt ttggctcgaa ggcggtcaga tcgccgaggc cttcggcgcc atgaaattcg
     1501 ccggcaccag cggggtcgcg caccagcggc cggtcggcgc cggcgtacgc cggcacgtcc
     1561 tcgcgcccgg cgatctggcg catgatgcgg gcgtttctcg cagtcgccgc gacggggacg
     1621 ttgccgccga cggtggtgat cgccagcagc tcaagctccg gcgaagcgaa ggcgaggaac
     1681 aggcccacgg cgtcgtcaac gccggggtcg caatcgatga tcagggcacg ggtcttcatc
     1741 gggttaggtt tggcgagggt cgcggcggag agcaagagca gcctgtctgg ttgatgccca
     1801 ttgacgacgg gcgtccggtc cggccccatg cggcttcagg gaaaagggct gtcatcatga
     1861 actccaaagt cgccgccggg ttggccacgc tactgctttc gaccaccacc ctgacgggat
     1921 ccggcgcagc gctcgcccag accgcgcccg tgctagcggc tacacccgtt cccaacccgg
     1981 cgttggccac gctgctgacc gattacgagg cgttcctgaa gaccaatgac ccgatctcgg
     2041 cagggttcga gggcgatcag gcggcgaaac ccaaactccc cgacgtcacc cgcgccgccg
     2101 aagtggccca gcaggcaccg ctggaggcct tcctcgcccg ggccgaagcc ctgccctcga
     2161 ccggcctctc gtccgacgac cggctgaacc gcgacttcct gatctgggtg ctgaagcgag
     2221 ccatcgagtc gatccgctac gacagcagcc ggctggcctt cacctccgag ggcgggattg
     2281 gccagaacat ggcctatgtg gcctcctcga cgcggatcac cagtcgctcc gacgccgacg
     2341 catggatggc gcggctggtg ggcctgccga aactctacga cgacagtctg gagaacgcgc
     2401 ggcgcggtct ggcgaccggc ttcacccagc cgaagtcggt gaccgagagc tatctggtct
     2461 ccctccgcaa cgaggccgct ttcacgccgg agaccgatcc gctgctgaag ccgttctccg
     2521 tcctgcccgc ctcgatcccc gctgccgatc aggcgacgct caaagcccgc gcagccgcca
     2581 tcgtcgctga acagatcacg cctcgtcgtc gccaatggct ggccttcgtg cagaatgact
     2641 atctgcccgc cgcctcggcg acagtcggga tcggctcgcg tccgggcggc aaggaatact
     2701 atgcgtttca ggcgcgctcc tacacgacta ccgacctgac gcccgaccag atccaccaga
     2761 tcggccttga cgaagtcacc cgcattcgcg cgcgtatgga tgtcgagatg cgggcttccg
     2821 gatggaccgg agatttcgcc ggcttcctga accatctgcg cactgacccg cagttctatg
     2881 cgacctcacg tgaggatatg atcgagaagg cgtcggagat ggccaagcgc gccgacggtg
     2941 gactgccggc cctgttccgc accttgccgc gcctgaccta caacgtccga ccagtgccgt
     3001 tggatatcga ggacacctat acgaccgggc gctacaacgg cgggtcgttg gccgagggcg
     3061 tacccggcgg ctatatcgtc aacaccggca agctcgacca gcggccgctg tatgaactgc
     3121 cggccctgac gctgcatgag gcggtgccag gccaccatat ccagatcgcc ctgcagcagg
     3181 aggcggcgca gggaccctat ttccggcgca acgtcgacgt caccgccttc accgagggct
     3241 gggggctgta ttcggagttt ctcggcgagg agatgggctt ctaccggacg ccagaagaac
     3301 gcttcgggcg tctgtcctat gaaatgtggc gggcgtgccg tctggtggcg gacaccggga
     3361 tgcacgcgat gggctggacc atcgaacagg cgcgccagtg ctttaccgac aactcggccc
     3421 tggcgccgca caacatcgag accgagttgc agcgctacat cggctggccc ggtcaggcgc
     3481 tggcctacaa gatcggcgaa atccgtctgc gcgccatccg gacacgggct gaaacggcgc
     3541 tgggcgacaa gttcaacgtc cgcgacttcc acgatgcgct gctggtcgat ggaccattgc
     3601 ccctcgacct gctcgacctg cggatggacg catgggtcgc tgctcggaaa gcggaataga
     3661 ccttagcgct tccggggcag gtcgatggcg gccagcttcc agccgaacac gttgcggcgc
     3721 tccagcagca ggtcgacata gtcgccgggc cggtcgtccc gcatcagccg caccgcgaag
     3781 gtgttcagtc cgcgatagct gtagccgtga cggaccttgg tcgagggctc attcggggca
     3841 cgagcacggt cgcgcgcgac atccctggcg ttggggactt cgcccgagcg gaccatggcg
     3901 gcgacggcgg gtggggtgac gaaagcgtcg acggcgccgc tgatcagggt cggggcgaag
     3961 atcatgccga gaccgctgag gccatcggcc cggtcgccca gatcggcgcg catctgttcg
     4021 accagtttgg agttcagctc ttccttcaga ctggtgcgaa aggcgggaaa atcgaccagc
     4081 ttctccagct tcgcctcgtc tcccaccttg gccgccgaga tcagcccgcg aacagccagc
     4141 aacggcgagg cgaaataggc ggcgaagaac aggaaaatga cgcccgcgag gacgatgagc
     4201 tgggtcttgc gattcattgg atctcctcga accgcaacgc ggcgcaggct tgacggtttc
     4261 gaggaaggct caaggcgcgg tcgatccttc gcgaggcagg ccaatatgca ccagcttcca
     4321 ctcatagggg ccgcgacgct cgaaggtgaa gactgtgggt gagtggatat catcagcgac
     4381 gctgagccgt gcccggttga cgctccagta ggtcggtcga ggccagcggc gcgcggtctc
     4441 gacagcggga ttggcgccga cagtcagtgc gtcgcggccg gagccggagg tcagggccag
     4501 caacgggccc ggggccagcc aggcatcgac cggtggcgcc gtcgagacga actgcggcgt
     4561 cgcctgttcg aactggcgac ggatggcgcc gatgggatcc tgcagtatgg acggcggtgg
     4621 ggtcagggct tcgacacgac cggacacttg gggcctcaac gaggcgcgta cggcatcgta
     4681 gtcgatcaac ttgcccaaac ccgcgacatc gcccgcctct gcggctgagc gaatgccgat
     4741 gaaggcgacg gcgggtgcaa ggaagaagct cacggccgcc agcacgatgc cgacgacgat
     4801 gaggttgagg atcaggcgtt tgaacgaagg ttcgcggcgg gccgccgccc gacgcgcctc
     4861 ggcctgcgcc cagcccggtc gatggtcgct ctcggtcgtg ggctcgtggc tcaagcgaag
     4921 atcctgacag gttcagcggt gacgctatgc ggtcaagaca acgcccgcaa cggtctagcc
     4981 gttgcgggcg tatgtttagt cacgagcggc gctcaatcag cgagcggggc ttcgttccgt
     5041 ttttgcgctt agccgacgtt cggcagctcg gcgcgcaggt tctcggcgac cttgtcgagg
     5101 aagccctcgg tggtcagcca gccctgttgg tcgccgacca gcagtgccag gtccttggtc
     5161 atgaaaccgg cttcgacggt gtcgacgacg atcttttcca gggtctcggc gaaggccgca
     5221 agttcggcgt tgtcgtcgag cttggcgcgg tgtttgaagc cggcggtcca ggcgaagatc
     5281 gaggcgatcg agttggtcga ggtggcctca cccttctgat gctggcggaa gtggcgggtc
     5341 acggtgccgt gggcggcctc ggtttccatc accttgccat ccggcgtcat cagcaccgag
     5401 gtcatcaggc ccagcgagcc gaagccctga gccaccacat cggactgcac gtcgccgtcg
     5461 tagttcttgc acgcccagac gaagccgccg gaccacttga tggccgcggc caccatgtcg
     5521 tcgatcagac ggtgttcgta ggtcaggccg gcggccttga acttgtcggc gtattcggcg
     5581 tcgaacactt cctggaagat gtccttgaag cgaccgtcat aggctttgag gatcgtgttc
     5641 ttggtcgaca gatagacggg gaagttgcgg ttcaggccgt agctgaacga ggcgtgggcg
     5701 aagtcgcgga tggaatcatc gaggttgtac atgcccatgg cgatgcccgc cgatgggaag
     5761 tcgaagactt cgtagttctg ctcttcgccg ttggcgccga cccacttcat ggtcagcttg
     5821 ccgggaccgg ggacgaggaa gtcggtggcc ttgtactggt cgccgaaggc gtgacgaccg
     5881 acgacgatcg gctgggtcca gcccggaacc agacggggca cgttctggca gataatcggc
     5941 tcgcggaaga cgacgccgcc gaggatgttg cggatcgtgc cgttcggcga cttccacatc
     6001 ttcttcaggc cgaactcctt cacccgggct tcgtcaggcg tgatggtcgc gcatttgacg
     6061 ccgacgccgt gcttctggat agcgtgggcc gcgtcgatcg tcacctggtc gtcggtggcg
     6121 tcgcggtgct ccatgcccag gtcgtagtag tccagatcga catcgacgaa cgggaagaca
     6181 agcttgtcct tgatccattg ccagatgatg cgggtcattt cgtcgccgtc gatatcgacg
     6241 atggggtttg cgaccttgat cttggccatg cggagcgcct cacgtttggg aaggaatggg
     6301 ttggcggcgg tatagccatc cggagccgtc ggcgcaaagg gtccgcgaaa agcgggtcgg
     6361 ggcatttccg gccggggtta accatgcccg caaaaaatca tccgcatacc atcgaaccag
     6421 atggggcccg gactcttctt atcgtgcctc ccacggcacg gagccccttc atggatcaag
     6481 agactgcggc gctggttcag cgctggatat tgacgtttct cgaacagccg cccctcatcg
     6541 acgcggaact gatgcgccac gtgctcgctg agcatgacaa ccgcgtcgca gagccctcgt
     6601 catgaccgaa aaaaccttca accccggcga tgccgtgaag tgggatcaca gccagggcac
     6661 gacgaccggc aaggtcattc tcaaggtgac aaagccgatg aagatcaagg accacaaggt
     6721 tgcagcttcg tcggaaaacc cggagtatct ggtcaagagc gacaagaccg gcgcagaggc
     6781 ggctcacaag ccatcggaac tgcgcaaggc ctgagacgtc gcagggggcg aacagaatgc
     6841 gcaaactctg gacgggcgcc gcggctctcg cgctcgcggc ctgcggcggt gagcagtcat
     6901 cggccaggcc ggctgaggcg acacagactc ctgcggcgac aagggccatt gcctcaaccg
     6961 ccaggtctga aagcaggaca tttcgcgact ggtacgccgt ctgcgacaac ggcaatctct
     7021 gcagcgccta ctcagggggc tcgacggggt ggatcctggt ccagatggaa gccggccccg
     7081 ccgctcagcc caccgtgcgc gtggggatgt ggcccgacaa cggcgaggaa ctgaagacgc
     7141 caattaccct ggtcatcgac ggcaagcgcc atgcgacgac gccggggccg gaggatacct
     7201 cgagcgccat gctaccggcg gcggacgccc gcgccgtggt tgcggaactg acagcaggac
     7261 gcgcggtcaa catatcgtcg ggcgaccaga cgatcgagtt gccggtcgcc ggagtgtcgg
     7321 cctccctgct gtggttcgac gagcgtcagg gtcgtc
//
LOCUS       DASVTM010000169         5490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_548516,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000169 DASVTM010000000
VERSION     DASVTM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548516"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VF633_04695"
     CDS             <1..639
                     /locus_tag="VF633_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8470388.1"
                     /translation="GLGARVETLQTDLSTEDGVQSLYDLVAGRPIDALAANAGHGLGE
                     GFLDQDFNDILHVINTNITGTTRLLHLIGRDMRARGRGQILLTGSIAGLMPGSFQAVY
                     NASKAYVDSFSFALRNELKDTGVSVTVLMPGPTDTEFFSRAGMDDTVVGQGKKMDPVD
                     VAKIGYHAMQRGEGDVVAGLMNKLQAATAAVTPQTALAEMHRKQAEPGGGSH"
     gene            complement(688..1125)
                     /locus_tag="VF633_04700"
     CDS             complement(688..1125)
                     /locus_tag="VF633_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470389.1"
                     /translation="MVAFLTGVGRDGANRTLDDVLALDDAALERHHDFIQWMFPLSEP
                     SRAVPGSPVLTEEDRLVISSSAIALANLAAGAARMSDFYDRTTDWLESHDHNHLRISR
                     IIRSLRLLIGDTAADQFRDARLADIAARNGRVNPLSLDHWQQS"
     gene            complement(1137..4430)
                     /locus_tag="VF633_04705"
     CDS             complement(1137..4430)
                     /locus_tag="VF633_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX8470390.1"
                     /translation="MTDPAKDAIVGEARLVDGPAPRTAGAMSHSRRASSALTAVVVVE
                     QLDAEAPLTVVLTTSERRADELGRAIAVMADPQVEVLVLPPWDCLPYDHASPSRDCQG
                     RRLEVLDRLDREDGTRRVLVVSPEAAMQRLAPAKARQAAFTLRTGDILDRDALQTFAA
                     RTGYVTDDRIDEPGEIAILGEVVDVFPSSAERPLRLTLDNDRIDTMRWFDPLTQRSEA
                     EIDVLTLMGASERYLEDQSREPGCEHRLAEDGPLESLFEMIDGAPLFAEPDAGDRCHA
                     FLEQVREAHQTARTFGDPAATLPAPTALYLAPDEAGRGLAKAKLLDVEGKATGVPTFT
                     LQRSPGRAFKAFLDDRLEAGHRVVVTGIAAELKMIARMLKRQGVEAPAAAAAWNEVLA
                     GDPGTLRAFDADLAAGFDAPHLRLSLITASDLLGGRISRTGAVAQGLIGEPELRVGDV
                     VIHEDHGLGILEALERIEIDGFERDVIRLGYHGGATVLAPVEEFGRIWRYGSEPSAVT
                     MDRLGSDGWRRRRAEVSAQIDQTASRLVELSAAREAARVEPIAPPRQPYARFSAGFPF
                     PESVDQNAAITAVLEDLASGRPMNRLVCGDVGFGKTEIALRAAAAVAMSGRQVVVAAP
                     TTVLARQHYETFRRRFRDTGLKVAHLSRLVDGAEAAETKAGLADGSVRIVVGTQALAA
                     ESLTFTDLGLVIIDEEQRFGTKMKDGLRDRAEHGLTMTATPIPRTLQSALVGLIDVSV
                     IASPPARRRPIRTTLTDFDGASLRTALLREKRRGGQSFVVCPRIEDIEPMAARLARLL
                     PELSVRTAHGKMPVAEVDAAMVGFAEGQDDILLATNIIESGLDVPRANTIVVWRPDRF
                     GLAQLHQLRGRVGRGRAQGFAYLLTEPADEIAEGTRARLGTLEAFDRLGSGFAISARD
                     LDLRGGGDIVGDEQAGHMKMIGSALYQQVLERAVRLARGEPTAEVLPPPRVDATTYLP
                     VDYIPDATMRINLYARLARIVSAAEVEVLEDEIADRFGPVPEPAAALLNATRLSALAA
                     EAGVTRIASGPKATAFTVASSRAALLRERLPANDERRWVEDRLVYDARKDRAHDSAFI
                     ASVLSDLAA"
     gene            complement(4903..>5490)
                     /locus_tag="VF633_04710"
     CDS             complement(4903..>5490)
                     /locus_tag="VF633_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470391.1"
                     /translation="AWDVHHFHLGTTITGGWAARTGPLLFARLTERDLYCIAVLPHGS
                     WTQQQLIATLHDNWPETIEMFRMKGVDGLAHQVSDDDLKALRKANINSATEVAPGVVY
                     MTPGLGAMLDGTSTWAMMSKLTLKRMCAELERQVRVSLSDPGSSISSALTANGIDPAY
                     ADFTLMFDGTNALLRERSTHVALNIGPQFAVPPID"
BASE COUNT         1030 a         1809 c         1706 g          945 t
ORIGIN      
        1 ggcctcggcg cccgtgtcga aacccttcag acggaccttt ccaccgagga cggcgtacag
       61 tccctgtacg acctcgtcgc gggccgcccg atcgacgcgc tggccgccaa cgcaggacac
      121 ggcctcggcg aaggttttct cgatcaggat ttcaacgaca tcctgcacgt catcaatacc
      181 aacatcaccg gcacgacccg gctgctgcat ctgatcggac gcgacatgcg cgcgcgtggg
      241 agaggccaga ttctgctcac cggctccatc gcggggctga tgcccggatc gttccaggcc
      301 gtttacaacg cctccaaggc ttacgtagac agcttttcgt tcgcgcttcg caacgagttg
      361 aaagacacgg gcgtcagcgt cacggtcctc atgccaggac caaccgacac agaattcttc
      421 tctcgcgccg ggatggatga cacggtggtc ggccagggca agaagatgga tccggtcgat
      481 gtcgcaaaga tcggctacca cgccatgcag cggggcgaag gcgacgtcgt ggccggcctc
      541 atgaacaagc tgcaggccgc gaccgccgcc gtgacaccgc agactgcgct cgccgagatg
      601 caccgcaaac aggctgagcc gggcggcggc tctcactgac gcgtgctggc gtcagcgcaa
      661 catgcgaaac tcaccgggta acgcaggtca gctttgttgc caatgatcga ggctgagggg
      721 attcacccga ccgttccgcg ccgcgatatc tgcaagccgt gcatccctga actgatcggc
      781 ggcggtgtcg ccgatcagca gtcgaaggct tcgaatgatg cgactgatcc gaagatgatt
      841 gtggtcatgg ctctccagcc agtcggtcgt acggtcgtaa aaatccgaca tccgcgcggc
      901 gccggcggcg aggttggcca gggcgatcgc cgaactgcta atcaccagac gatcctcctc
      961 cgtcaacacg ggggatcctg gaacagcccg gctgggttcg gagaggggaa acatccactg
     1021 aatgaaatcg tggtggcgtt cgagcgccgc atcgtcgagc gccagtacgt cgtctaacgt
     1081 ccggttggcg ccgtcacggc ccacgccggt cagaaaagcg accacaggcg agcccatcag
     1141 gcggccaggt ctgacaggac cgaggcgatg aaggcggagt cgtgcgcgcg gtctttacgg
     1201 gcgtcataga caagccggtc ttcaacccat cggcgctcat cgttggcggg aaggcgctct
     1261 cgcaacaggg ccgcccgcga agaggcgaca gtgaacgccg tcgccttcgg gccggaggcg
     1321 atgcgcgtga cgccggcttc agccgcgagc gctgacaacc gggtcgcgtt caacagggcg
     1381 gccgccggct ccggaaccgg cccaaatcga tcggcgatct cgtcttcaag gacctcgacc
     1441 tccgcggccg acacgatgcg ggccagtcgg gcatacagat tgatgcgcat cgttgcgtca
     1501 gggatgtagt ccaccggcag ataggttgtc gcatccaccc ggggcggtgg aagaacctct
     1561 gcggtcggct cgccacgcgc caaccttacc gctcgctcca gcacctgctg atagagagcc
     1621 gatccaatca tcttcatatg accggcctgc tcgtcaccga cgatatctcc accccctctc
     1681 aggtcgaggt cgcgagcgct gatcgcgaag ccggacccca gccggtcgaa ggcctccaga
     1741 gttcccagcc gggcgcgggt tccctccgca atctcatcag cgggttcggt cagaagatag
     1801 gcgaaaccct gcgcccgccc gcgtccgacc ctgcctcgca actggtgcaa ctgggccagc
     1861 ccgaagcgat ctggccgcca gaccacgatg gtattggcgc gcggaacgtc cagaccgctc
     1921 tcgatgatgt tggtggccag caggatatcg tcctgtccct ccgcaaaccc gaccatcgcg
     1981 gcatccacct cggccaccgg catcttgccg tgagccgtac gcacgctcag ttccgggagc
     2041 agccgggcca atcgcgcagc catcggttcg atgtcctcga tccggggaca aacgacgaaa
     2101 ctctgcccgc cccgccgctt ctcgcgtagc agggcggtgc gcagcgaagc gccgtcaaaa
     2161 tcggtgaggg tggtccggat cggccggcgc cgggcgggcg gactggcgat cacgctgaca
     2221 tcgatcaggc cgactagggc gctctggagc gtgcggggga tcggggtcgc ggtcatggtc
     2281 agaccgtgct cggcccgatc ccgcaggccg tccttcatct ttgtgccgaa ccgctgctct
     2341 tcatcgatga tgacgagccc cagatccgtg aacgtcagcg attccgcagc cagggcctgg
     2401 gttcccacga cgatacgcac cgagccgtcg gccagtccgg ccttcgtttc cgcagcctcc
     2461 gctccgtcga ccagacgcga caggtgcgcc actttcaggc cggtgtcgcg gaagcgtcgc
     2521 ctgaaggtct cgtaatgctg ccgggccagc actgtggtcg gcgcggcgac gacgacctgg
     2581 cgcccggaca tggcgaccgc cgctgcagcg cgcagggcga tttcagtctt gccgaagcca
     2641 acatcgccac agaccaacct gttcatcggt cgtccgctgg cgaggtcttc cagaaccgcg
     2701 gtgatcgctg cattctggtc gacggattca ggaaacggaa agcccgcaga gaacctggca
     2761 tagggctggc gcggcggagc gatcggctcg accctggccg cctcgcgggc tgcagagagt
     2821 tcgaccaacc gggacgcagt ttgatcgatc tgtgcgctca cttccgcgcg ccggcggcgc
     2881 cagccgtcag aaccaagccg gtccatggtc acggcagagg gctccgaacc gtatcgccag
     2941 atacggccga actcctcgac cggcgcaagc acggttgcgc cgccgtggta accgagccgg
     3001 atcacatcgc gctcgaaccc gtcgatctcg atccgctcga gcgcttcgag gatgccgagc
     3061 ccgtgatctt cgtgaatgac cacgtcgccc acgcggagtt ccggttcgcc gatcagaccc
     3121 tgggcgacag ctcccgtgcg cgatatgcga ccgcccagaa ggtcgctcgc ggtgatcagt
     3181 gaaaggcgca gatgcggagc atcgaaccct gccgccagat cggcgtcaaa agcccgaagc
     3241 gtcccggggt cgccggcgag aacctcgttc caggccgccg ctgcggcggg cgcctcgacg
     3301 ccctgacgtt tgagcatacg agcgatcatc ttcaactcgg cggcgatacc tgtaacgacg
     3361 acccgatgtc ctgcctccaa tcgatcgtcc aggaacgcct tgaacgcccg gccagggcta
     3421 cgctggagcg taaaggtcgg aacacctgta gccttgccct cgacatccag gagcttcgct
     3481 ttcgccagac cgcgtccggc ctcgtccgga gccagataga gggcggtcgg tgcgggaagt
     3541 gtcgctgccg ggtccccgaa cgttctggcg gtctgatgcg cttcccggac ctgttccaga
     3601 aacgcgtgac atctgtcccc tgcgtccggc tcggcgaaca gcggagcccc gtcaatcatt
     3661 tcaaacaggc tctccagagg cccgtcctcg gcgagtcgat gttcgcaacc cggttctcgc
     3721 gactggtcct ccagataacg ctcggaggcg cccatcaggg tcaggacgtc gatctccgcc
     3781 tctgagcgtt gcgtgagcgg atcgaaccac cgcatcgtgt cgatgcgatc gttgtccagg
     3841 gtgaggcgaa ggggtcgttc cgccgaggac ggaaaaacat cgacgacctc gcccaggatg
     3901 gcgatttcac cgggctcgtc gatccggtcg tcagtaacgt agccggtccg cgccgcgaag
     3961 gtttgcaagg catcgcggtc gaggatgtcg ccagtcctca gcgtgaacgc ggcctgccgc
     4021 gctttcgcag gcgcaagccg ctgcatggcc gcctccgggc tgacgaccag cacccgtcgg
     4081 gttccatcct cgcgatccag acggtccaga acttcaaggc ggcgaccctg acagtcacgc
     4141 gacggtgagg cgtgatcata gggcaggcaa tcccatggcg gcagaaccag aacctcgacc
     4201 tgcgggtcag ccatgaccgc gatggcgcgt ccaagttcat cggcccggcg ctcgctggtc
     4261 gtcagcacca ccgtgagagg cgcttcggca tcaagttgct cgaccacaac gacagcggtg
     4321 agagccgaac tggcacggcg cgagtgactc atcgccccgg cggttcgcgg cgcggggcca
     4381 tcgaccagcc gcgcttcgcc gacgatcgca tcttttgcag ggtctgtcac aggcgtcggt
     4441 cctcctgcgg agacaacacg agcccggccg tgcggctccc tcaaatcgcg ggcgaaacag
     4501 aaaaattgtc ggggttaaac cgaggtcctg aagcgcaagg acagccgtga cgagaacaga
     4561 ccccggagag cgtctgatcg tcgatccatg ccacgtcgtt ggaaaaatgg tgccgcttag
     4621 gtgactcgaa cacctgaccc ccgcattacg aaggctactc caccctcggc cggaaagcct
     4681 tgcaggcaat ggttctggac caaattttac cgtggcccgg cactcctacc cagacccttc
     4741 ccagactggc ctgagggagc ggtggaggcg tttcctaagt tacgcgcttt aacgtgggaa
     4801 ccgccgaaat ttcgacctgc gctatccctg cggaagccca ctttcgtcgg ccgccgtttc
     4861 ctcgtccaac ttactgccgc cctttggagc gtccgccacc gttcagtcga tgggcgggac
     4921 agcgaactga gggccgatat tcaaagcgac gtgcgtactg cgctcgcgca gcagcgcgtt
     4981 ggtgccgtca aacatcaggg tgaagtccgc gtatgctgga tcgatgccat ttgcggtgag
     5041 cgccgatgag atcgacgatc ctggatcact caagctaacc ctaacttgtc gttccagctc
     5101 ggcgcacatc ctcttcaagg tgagtttgga catcatcgcc cacgtgctgg ttccatccaa
     5161 catcgcccct aggccaggtg tcatatagac tactccggga gcgacttcag tagcggagtt
     5221 gatgttggcc ttcctcagtg ccttcaagtc atcgtccgac acttggtgag cgaggccatc
     5281 tacgcctttc atgcgaaaca tctcgatggt ttcgggccaa ttgtcgtgca gcgtagcgat
     5341 tagctgctgc tgcgtccaag agccatgagg caagaccgca atgcagtata gatcgcgctc
     5401 tgttaaccga gcgaacaaga gcggaccggt ccgcgcagcc cagccgcctg tgattgttgt
     5461 gccaagatgg aagtggtgaa cgtcccatgc
//
LOCUS       DASVTM010000170         6042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_709867,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000170 DASVTM010000000
VERSION     DASVTM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709867"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VF633_04715"
     CDS             <1..1324
                     /locus_tag="VF633_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470392.1"
                     /translation="VRADLPAAERPTMRATVLMLLLLLGACATPIRPAADPTGDSLDA
                     IAHDYVALTLEIGEREPGYVDAYYGPAEWQAAAKANPRTVPQLIQGAAGLTQRLGAVP
                     HRGLDEASLQRRKYLKAHVSAASARLRMLSGEKMSFADEAKALFGIRPELKPLSDYEP
                     ILARIDALVPGTGPLTDRITTFKAGFVIPKDRLEPVMAAAIAECRRRTAQHIALPANE
                     RFTLALVTDKPWSGYNYYQGDAASKIEINTDFPIYTERAIDLGCHEGYPGHHVYNALL
                     ERTFVKQRGWVEMSVYPLFSPMSFVAEGSANYGIDLAFPGSEGAVFERNVLMPLAGLP
                     PADVEAKAELGRLTHELARAEYTIADDYLSGRVGKDQTVARLSKYLLTPPDKAAQRLR
                     FIDTYRSYIINYGLGRDTVEQWVARQGPDHWRGMETLLSSQILPVDLK"
     gene            1493..2014
                     /gene="lspA"
                     /locus_tag="VF633_04720"
     CDS             1493..2014
                     /gene="lspA"
                     /locus_tag="VF633_04720"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX8470393.1"
                     /translation="MKIPTHIPRIAWAAYGFALAIVVLDQLSKAWILDGLELREVGRV
                     FVFPPIFNVSYVENTGVSFGLFGGGSARWMLSVFSIVVAAGLAWWATQADRRLLATAI
                     GFVIGGALGNAIDRIRFGYVVDFLDFSGTGVFPWVFNIADSAISIGVVLLILDSVLSD
                     RKAAQVGVVHEKS"
     gene            2079..2645
                     /locus_tag="VF633_04725"
     CDS             2079..2645
                     /locus_tag="VF633_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HEX8470394.1"
                     /translation="MRIRSVALVSLLAVSTVALGACGGLKQSIGLTKVVPDEFVTVST
                     APLSVPPEYGLRPPAPGQPRPQELAPESAARQILLGQRQSITRTPGENALVTAAGAER
                     ADPLARYVVDDEFGDLTHKEESFANRVLFWRKDDAATQAPTVSQTSAGAVTIDAASET
                     ARLQALTGNRAGIAIQPRRDSRFKLPGL"
     gene            2750..3202
                     /locus_tag="VF633_04730"
     CDS             2750..3202
                     /locus_tag="VF633_04730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8470395.1"
                     /translation="MSESITATFADLSGVEQLRLGLEGKAPVAPIARTVGFELVEVEE
                     GRVVFEAVPTKAVYNPLGTVHGGWMATILDSACGCVVHSMLRPGQSYTTLELKTVFHK
                     ALKADTPVRAVGKIVQMGRRAAFSEAELRGTDGKLYATATSTCLVMEQ"
     gene            complement(3260..4051)
                     /locus_tag="VF633_04735"
     CDS             complement(3260..4051)
                     /locus_tag="VF633_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8470396.1"
                     /translation="MTDRSGRVAGKTALITGAAGGLGEAMAWMLAREGSKIALTDVDG
                     GRAETLAAAINAEVPGAAFAYAHDVRSEDQWIEVVARAAADMGGLSVLVNNAGVGDVL
                     LFAEQDTTENWQRQYEINLRSVMFGCKHAMPHLRDNAPSSIINISSIAGLAGAPGMGA
                     YNATKAGVWMYTKTIALEAAKMEWNVRVNSVHPVFIKTPILDPFIAMAHGDEAKAHER
                     LARGIPLKRIGEPDDVAYAVLYLASDESKFVTGSEFKIDGGMTAQ"
     gene            complement(4048..4353)
                     /locus_tag="VF633_04740"
     CDS             complement(4048..4353)
                     /locus_tag="VF633_04740"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX8470397.1"
                     /translation="MAQMPVDVAETLKGLPLWSTAEDLRPAIARTLIFKDFNAAFGFM
                     TRVALMADKMDHHPEWSNVYNRVSVVLTTHDAGGLTDLDIKMARFIDEAAKSLGAKE"
     gene            4463..5194
                     /locus_tag="VF633_04745"
     CDS             4463..5194
                     /locus_tag="VF633_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8470398.1"
                     /translation="MTIVEGMMDNLWLAGLAPADRRRIAPHLNEKSFAQGQVIYDAGE
                     AVDEVWFPLRGVVSLMTLLPDDKMIETAAIGREGLVGVTCGPMNARASSRAISQSDGV
                     AVTLPNAVFTDALAHSETMRMSLARYTEGLFAQVQQTAACNAQHRLDERLARWLLTIH
                     DRSDEDRFDLTQSDIAGMLGVRRATVSEVGAALEAKRLITRGRGWVEVTNRQALEHAS
                     CGCYRMMRSVMTDLGLPLAAQPEPA"
     gene            5205..5978
                     /locus_tag="VF633_04750"
     CDS             5205..5978
                     /locus_tag="VF633_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8470399.1"
                     /translation="MRIATFNINGVNSRLGSLLDWLADARPDVACLQELKATDVQFPA
                     KAIADAGYHAVWQGEHRWNGVAILSRDAEPIVTRRALPGDPRDAQSRYLEAAVNGVLI
                     ACLYQPNGNPRPGPKFDYKLAWAERLNAHAATLFGTGAPVVLAGDYNVVPTDADIYNT
                     RSWKKDALLQPESRAAFARLLEQGWTDALDERFPNDPQWTFWSYFRESWARDAGLRID
                     HLLLNGPAAERLTDAGVDRAVRGQDHASDHAPTWIELAD"
BASE COUNT         1043 a         2053 c         1867 g         1079 t
ORIGIN      
        1 cgtccgcgcc gacctgcctg ccgccgagag accgaccatg cgcgcgaccg tcctgatgct
       61 gctgctcctg ctgggggcgt gcgccacgcc gatccgaccc gcggccgatc cgaccggcga
      121 cagcctcgac gccatcgccc acgactatgt cgccctgacg ctggagatcg gcgaacgcga
      181 gcccggctat gtcgacgctt actacggccc ggctgaatgg caggcggcgg ccaaggcgaa
      241 cccccgcacc gtgccgcaac tgattcaagg tgcggcgggc ctgacccagc gcctcggcgc
      301 agtgccgcat cgcgggctgg atgaggcaag cctgcaacgc cgcaaatatc ttaaggcgca
      361 cgtgtcggcg gcatctgctc gactgcgcat gctgtccggc gagaagatga gttttgcgga
      421 tgaagccaag gctttgttcg gcatccgacc cgaactgaag cccttgtcgg actacgaacc
      481 catccttgcc cgcatcgatg cgctggtccc cgggaccgga ccgctgacag accgcatcac
      541 tacgttcaag gccggcttcg tcatccccaa ggatcggctc gaaccggtca tggcggccgc
      601 aatcgccgaa tgccgtcgcc gcacggccca gcacatcgcc ctgcccgcca acgagcgctt
      661 cactctggct ctggtcacgg acaagccgtg gtccggctat aattattatc agggcgacgc
      721 cgcctcgaag atcgagatca acaccgattt cccgatctac accgagcggg cgatcgatct
      781 cggctgccac gagggctacc ccggccacca cgtctacaac gcccttctcg agcggacctt
      841 cgtcaagcag aggggctggg tcgagatgag cgtctatccg ctgttctcgc ccatgtcgtt
      901 cgtggccgag ggcagcgcca actacggcat cgaccttgct tttcccggca gcgagggcgc
      961 cgtattcgag cgtaacgtgc ttatgccgct tgccggtctg ccgcccgcag acgtcgaggc
     1021 gaaggctgag ctcgggcggt tgacgcatga actggcgcgc gccgaataca ccatcgccga
     1081 cgactatctg tcgggtcgcg tcggcaaaga ccagaccgtc gcccgcctgt cgaaatatct
     1141 gctgactccg cctgacaagg ccgcgcaacg gctgcggttt atcgacacct accgcagcta
     1201 catcatcaac tacggcctcg gccgcgacac ggtggagcaa tgggtcgccc gccagggccc
     1261 cgaccactgg cgcggcatgg agacgctgct gtcgagccag attctgccgg ttgatctgaa
     1321 gtagggagga ggcagtaggc aggtaggcag taggcaaaaa ggcttggcgg ttgagagtac
     1381 cgcgccgccg gcgacacgca atatcggcgc atcgccccta cctcactgcc taccgcctac
     1441 tgcctactcc tgccttcccc cgccttccct cgcctgctaa gccccgggcc gcatgaaaat
     1501 accgacccac attccgcgta tcgcctgggc cgcctacggc ttcgctctcg ccatcgtcgt
     1561 gctcgaccag ctcagcaagg cctggatcct cgacgggcta gagctgcgcg aggtcggtcg
     1621 ggtgttcgtc ttcccgccga tcttcaacgt cagctatgtc gagaacaccg gcgtcagctt
     1681 cggcctcttc ggcggcggct cggcccggtg gatgctgagt gtcttctcga tcgtcgtcgc
     1741 cgcaggtctg gcctggtggg ccacgcaggc cgaccgtcgc ctgttggcta ccgccatcgg
     1801 gttcgtgatc ggcggtgcgc ttggcaacgc catcgaccgc atccgcttcg gctacgtggt
     1861 cgattttctc gacttttcgg gcaccggcgt gttcccgtgg gtgttcaaca tcgccgacag
     1921 cgccatcagc atcggcgtgg tcctgctcat cctcgacagc gttttgtcgg atcgcaaggc
     1981 agcgcaggtt ggcgttgtcc acgaaaagtc gtaatcagcc gctcttgagt atttcgcaga
     2041 gcgcgcacct tcaggcgcgg ccggagttca gccctaccat gcgtattcgc agcgtcgccc
     2101 ttgtcagcct tctcgcggtc tcgaccgtgg cgctcggcgc ctgtgggggt ctcaagcaaa
     2161 gcatcggtct gaccaaggtc gtgccagacg agttcgtcac cgtttccacc gctccgctca
     2221 gcgtgccgcc cgagtacggc ctgcgtccgc cggcaccggg ccagccgcgc ccgcaagagc
     2281 tcgcgcccga aagcgccgcc cgccagatcc tgctgggtca gcgtcagtcg atcacccgca
     2341 cgccgggcga gaacgccctg gtcacagcag ccggcgctga acgcgctgac ccgctggcgc
     2401 gctatgtggt cgacgatgag ttcggcgacc tgacgcacaa ggaagaaagc ttcgccaacc
     2461 gcgtgctgtt ctggcgcaag gacgatgcgg cgacgcaggc tccgaccgtc agccagacat
     2521 ctgcgggcgc cgtcaccatc gacgccgcca gcgagaccgc tcgtcttcag gccctgaccg
     2581 gtaaccgcgc cggcatcgcc atccagccgc gccgcgacag ccgcttcaag ctgccgggcc
     2641 tttaggctcg cagcctcacg ttttctaaga ctgcaaagga cgcgcttgcc accggcttgt
     2701 gcgtccttat tgtgtacgga ccccccccgc atcctccccg gagcgtcgaa tgtccgaatc
     2761 catcaccgcc accttcgctg acctgtcagg cgtcgagcag cttcggctcg gcctagaggg
     2821 aaaagcgccg gtcgcgccga tcgcccgaac cgtcggcttc gaactggtcg aggttgagga
     2881 aggccgcgtg gtgttcgagg ctgtcccgac caaggcggtc tacaacccgc ttgggaccgt
     2941 tcacggcggc tggatggcga cgatcctaga ctcggcctgt ggctgcgtgg tccattccat
     3001 gctcaggccg ggccagagct atacgacgct ggagctgaag accgtcttcc acaaggcgtt
     3061 gaaggccgac accccggtcc gcgccgtggg gaagatcgtg cagatgggcc gccgcgccgc
     3121 cttctccgag gctgaactgc gcggcacgga cggcaagctg tatgcgacgg cgacgtcgac
     3181 gtgtctggtg atggaacaat agagaagaac cctaatgccg cctgtcggcc tccctttccc
     3241 accgggaaag ggattggtct tattgcgcgg tcatgccgcc gtcgatcttg aactctgacc
     3301 ctgtcacgaa tttgctctcg tccgacgcga gatagagcac cgcatacgcc acgtcgtcag
     3361 gctcgccgat ccgcttaagc ggaatgccac gggccagccg ttcgtgggcc ttggcctcgt
     3421 cgccgtgggc catggcgatg aagggatcga ggatcggggt cttgatgaag acagggtgga
     3481 cagagttgac ccgcacgttc cattccatct tggcggcctc gagcgcgatg gtcttggtgt
     3541 acatccagac ccccgccttg gtggcgttat aggcgcccat gccaggcgct cctgccagtc
     3601 cagcgatcga cgagatgttg atgatggacg acggagcgtt gtcgcgaaga tgcggcatgg
     3661 cgtgcttgca gccgaacatc accgagcgga gattgatctc gtactgacgc tgccagttct
     3721 ctgtcgtgtc ctgttccgca aaaagcagga cgtcgccgac tccggcgttg ttcaccagaa
     3781 ccgacaaccc gcccatgtcc gcagcggcgc gggccacgac ctcgatccac tggtcctctg
     3841 agcgcacgtc atgggcatag gcgaacgccg cgcccggaac ctcggcgttg atggcggcgg
     3901 cgagggtctc ggcgcggcct ccgtcaacat cggtcagggc gattttcgag ccttcgcgcg
     3961 ccagcatcca tgccatggcc tcgcccaatc ctccggcggc tccagtgata agcgccgtct
     4021 tgcccgcgac gcgtcctgat ctgtcggtca ttctttcgct cccagcgact ttgcagcctc
     4081 gtcgatgaaa cgggccatct tgatatcgag atcggtgagg cccccggcgt catgcgtggt
     4141 caggacgacg ctgacccggt tgtagacgtt cgaccattcg ggatgatgat ccatcttgtc
     4201 ggccatcagg gccacgcggg tcatgaagcc gaatgcggcg ttgaaatcct tgaatatcag
     4261 tgtgcgggcg attgccggac gtaggtcttc ggcggtgctc caaagcggca gacccttcag
     4321 ggtttccgca acgtcaacag gcatttgcgc cattttatgt ccctcgcctc cctccgccgt
     4381 agcgcgcgcc gggcctctct acaagccatg ccaaggtcgg gttctggaca gaggaacgca
     4441 cggccaagct ttggcgttac cgatgactat tgtggagggc atgatggaca atctctggct
     4501 cgccgggctc gcgcctgcag accgccggcg gatcgcaccg catctgaatg aaaaatcttt
     4561 cgctcagggc caggtgatct atgacgccgg cgaggccgtc gacgaggtct ggtttccgct
     4621 caggggcgta gtctcgctga tgaccctgct gccggacgac aagatgatcg aaacggccgc
     4681 catcggccgc gaaggtttgg tcggcgtgac ctgcggcccg atgaacgcgc gcgcttcgag
     4741 ccgcgccatc tcgcagtcgg atggtgtagc cgtcaccctt cccaacgcag tctttaccga
     4801 cgctctcgcg cacagtgaga ccatgcggat gtctctcgcc cgctataccg aaggtctctt
     4861 cgctcaggta cagcaaaccg ccgcttgcaa cgcccagcat cggctggacg aacggctggc
     4921 ccgctggctt ctgacgatcc acgaccgctc ggacgaggac cggttcgatc tcacccagtc
     4981 agacatcgcg ggcatgctgg gcgtgcgccg cgccacggtc agcgaggtcg gcgccgcgct
     5041 cgaggccaag cgcctgatca cacggggtcg cggatgggtc gaggtcacca accgccaagc
     5101 tctggaacac gcctcgtgcg gttgctaccg gatgatgcga agtgtgatga ccgatctcgg
     5161 cctccccctg gccgcgcagc ctgaacccgc atgaagacat ttcggtgagg atcgccacct
     5221 tcaacatcaa tggcgtaaac agccgattgg gcagcctgct ggattggctc gccgatgcga
     5281 ggccggacgt cgcctgcctc caggagctga aagccaccga cgtccagttc cccgccaagg
     5341 ccatcgcaga cgccggctac cacgccgtgt ggcagggcga gcatcgttgg aacggggtgg
     5401 ctatcctgtc gcgggacgcc gagccgatcg tcactcgccg cgccttgccg ggcgacccca
     5461 gggacgccca aagccgatat ctggaggcgg ctgtgaacgg cgtcctgatc gcctgcctgt
     5521 atcagccgaa cggcaacccc cggccgggac cgaagttcga ctacaagctg gcgtgggccg
     5581 agcggctgaa cgctcacgcc gccaccttgt tcgggaccgg ggctccggta gttctggccg
     5641 gtgactacaa cgtcgtcccg accgacgccg acatctacaa cacccgctcg tggaagaaag
     5701 acgccttgct gcagcccgag agccgcgccg ccttcgcccg gttgttggag cagggatgga
     5761 ccgacgcgct ggacgagcgg ttccccaacg atccccaatg gaccttctgg tcctatttcc
     5821 gtgaatcatg ggcgcgcgac gcgggcctca gaatagatca cttgctgctc aacggccccg
     5881 ccgccgaacg cctgaccgac gccggggtcg accgagccgt gcgcggtcag gaccacgcca
     5941 gcgaccacgc tccgacatgg atcgaactcg ccgactagtt ctcgtcaaat ccgaccacgc
     6001 gagccggtga cgcgggcctc gccagaggct aaaccgccat ca
//
LOCUS       DASVTM010000171         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_710100,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000171 DASVTM010000000
VERSION     DASVTM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710100"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VF633_04755"
     CDS             <1..1404
                     /locus_tag="VF633_04755"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8470400.1"
                     /translation="EHVAPADHADFYSASRFTLNVTRADMVKAGFSPSIRLFEAAACE
                     TPIISDVWEGLDTLFQPGTEIVLALTADDVARALSVDEPARQAMAAGARRRVLDHHTA
                     DQRAVTLEHELHAAIQRRSARPRVDTARDITMRPTSARRVLVAGGAGFLGSHLCDRLL
                     AQGCEVLCLDNLLTGSLDNLKDALGHPNFEFIKADIVEPLPERIASRSFDRIYNLACA
                     ASPPLYQADPQHTLLTSVVGTKHLLDLAETTGARFLLTSTSEIYGDPTVHPQPETYLG
                     NVNCTGPRACYDEGKRCAETLTFDYDRAGRGEVRVARIFNTYGPRLSAADGRVVSNLV
                     SQALANAPITVFGDGSQTRSFCYVEDMISGLIALMEHAGPQPGPINLGNPTELTVSEL
                     VGVIQQLTGSSSPVTHNPLPVDDPKRRRPNIDKADAILKWRPQVNLSQGLQATIAWFD
                     AEATQARSVAEPRRQTA"
     gene            1401..2402
                     /locus_tag="VF633_04760"
     CDS             1401..2402
                     /locus_tag="VF633_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8470401.1"
                     /translation="MNDIMTAASPSRLATMRAAVVTGPGRVEVQTLARPEPGPRQVRV
                     RMEGCGVCASNLTPWAGPDWMNFPTEPGGLGHEGWGVVDAVGDGVTEAAIGQRVATLS
                     HHSYAEYDLADAGQVVPLPDSLADKPFPGEALGCAMNIFRRSDIHAGQTVAIIGIGFL
                     GAILTRLASEAGARVIAISRRPSSLQLARDMGAAETLPMDDHYALISKVAAMTDGLFC
                     DRVIEAVGKQWPLDLAAELTREHGRLIIAGYHQDGPRQVNMQLWNWRGLDVVNAHERD
                     PAMYARGVREAVEAVASGRLDPTPLYSATYNLDQLDEALDATRDRPAGFVKALVTFA"
     gene            2399..3397
                     /locus_tag="VF633_04765"
     CDS             2399..3397
                     /locus_tag="VF633_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8470402.1"
                     /translation="MIALGNAPRVGFLGVGWIGRHRMEAILKAGDVDAVAIVEPQDEL
                     AAAALALAPAATRVDSLDALLALDLDGIVIATPSAAHAAQSIQALDAGKAVFCQKPLG
                     RTAAEVRSVVEAARRADRLLAVDLSYRFTEGAQQLCALVREGALGPLHAIDLTFHNAY
                     GPDKPWFYDRALSGGGCVMDLGVHLIDLALWMLPGAAVVDVTSNLFSGGRPLTNTSAE
                     VEDFAVATLTLDTGAVVRLACSWNLPAGQEAVISASAYGAHGGGALRNVNGSFYDFTA
                     ERYVGTSTEILSTPPDDWGGRAAIEWASRLRRGQRFDPEIESIVAVADAIDRIYDR"
BASE COUNT          702 a         1352 c         1226 g          672 t
ORIGIN      
        1 gagcatgtag cgcctgccga tcacgccgac ttctacagcg cgagccgctt caccctgaat
       61 gtcacgcgcg ccgacatggt caaggcaggt ttcagcccca gcattcgcct gttcgaggct
      121 gcggcctgcg agaccccgat catttcggat gtctgggagg gactggacac cctgttccag
      181 ccgggaacag agatcgtgct ggcccttacc gccgacgacg tcgcccgcgc gctgtccgta
      241 gacgaaccgg ctcgacaggc catggccgcc ggggcgcggc gtcgtgttct ggatcatcat
      301 accgccgacc agcgcgccgt cacgctcgaa cacgaactgc acgcggccat ccagcggcgg
      361 tccgcccgcc cgcgcgtcga caccgccagg gacatcacca tgaggcccac ctctgcacgc
      421 cgcgtcctcg tcgctggagg cgcaggcttc ctcggctccc atctgtgcga caggctgctc
      481 gcccaaggct gtgaggtcct gtgcctagac aatctcctga ccgggtcgct cgacaacctg
      541 aaggacgcgc tgggccaccc gaacttcgag tttatcaagg ccgacatcgt cgaacccttg
      601 cctgaacgca tcgccagccg gtctttcgac cgcatctaca atctcgcctg cgcggcgtcg
      661 ccgcctctct accaggccga tccgcagcac acgctcctga ccagcgtggt cggaaccaaa
      721 cacctgctgg acctggctga aacgacgggc gcccggttcc tgctgacgtc gacgagcgaa
      781 atctatggcg atccgacggt tcacccccag ccggagacct acctcggcaa tgtgaactgc
      841 accggtcccc gggcctgtta cgacgaaggc aagcggtgcg ctgaaacctt gaccttcgac
      901 tacgatcggg cgggccgcgg cgaggttcgg gtggcccgga ttttcaacac ctacggccca
      961 cgtctttcgg cggctgatgg ccgggtcgtc tccaaccttg tcagtcaggc gctggcgaat
     1021 gcgcccatca ccgtctttgg cgacggatcc cagacccgtt cgttctgcta cgtcgaggac
     1081 atgatcagcg gcctgatcgc cctgatggag cacgcgggac cccagcctgg cccgatcaat
     1141 ctcggcaatc cgaccgagtt gacggtgagc gaactggttg gcgtgatcca gcaactgacc
     1201 ggctcaagct cgcccgtcac gcacaaccct ctgccggtcg atgatccaaa acgccgccga
     1261 ccgaatatcg ataaggcgga cgccatcctg aaatggcggc cccaggtcaa cctgtcgcaa
     1321 ggactgcaag cgaccatcgc ctggttcgac gctgaagcca cgcaagcccg atccgtagct
     1381 gaacccagga ggcaaaccgc atgaatgata tcatgacagc agcctccccc tcgcgcctgg
     1441 cgacgatgcg cgcagccgta gtcacagggc ccggtcgggt ggaggttcag accctcgcgc
     1501 gccccgagcc cggcccacga caggtgcgtg tgcggatgga aggatgcggg gtctgcgcca
     1561 gtaacctgac gccctgggcc ggaccggact ggatgaattt cccgaccgaa cccggcggtc
     1621 tcgggcacga aggctggggc gtcgtggacg ccgtcgggga cggtgtgacg gaggccgcca
     1681 ttggccagcg cgtcgccacc ctgtcccacc acagctatgc cgaatacgac ctcgccgacg
     1741 ccggtcaggt tgtgcccctg ccggatagtc tcgccgacaa gccctttccc ggcgaagcgc
     1801 tgggatgcgc gatgaacatc tttcgtcgca gcgacatcca cgctggccaa accgtcgcaa
     1861 tcatcggcat cggctttctc ggcgcgatcc tgacccgtct ggcgtccgaa gccggcgcgc
     1921 gggtcatcgc catttcgcgg cgtccgtcct ccctgcagct cgcccgtgac atgggcgccg
     1981 ccgaaaccct gccgatggac gatcactatg cgctgatcag caaggtcgca gccatgaccg
     2041 acggcctctt ctgcgaccgg gtcatcgagg ctgtcggcaa gcagtggccg ctcgacctcg
     2101 ctgccgagtt aacgcgtgaa cacggccgat tgatcatcgc cggctatcat caggacggtc
     2161 cgcgtcaggt gaacatgcag ttgtggaact ggcgcggcct cgatgtcgtg aatgcgcacg
     2221 aacgcgaccc cgccatgtac gcacgcggcg tccgcgaggc ggtggaagcg gtcgcttcag
     2281 gccggctcga tccgacgccg ctctattccg ccacctataa tctcgaccag cttgatgaag
     2341 ccctcgacgc cacccgcgat cggccggcgg gcttcgtcaa ggcgctggtg accttcgctt
     2401 gatcgcgcta ggcaacgctc cacgcgtagg atttctcggc gtcggatgga tcggccggca
     2461 ccggatggaa gcgatcctga aagccgggga tgtcgacgcc gtcgccatcg tcgagcctca
     2521 ggacgaactc gcagcggccg cgctggcgct cgctcccgca gctactcggg tcgacagtct
     2581 ggacgccctg ctcgcgctcg atctggacgg gatcgtcatc gctacaccga gcgccgccca
     2641 cgccgctcag tcgatccagg cgctggacgc cggtaaagcc gtcttttgcc agaagccgtt
     2701 aggccgaaca gctgccgagg tgcgctcggt cgtagaggcg gcgaggcgag cggatcgtct
     2761 gctcgccgta gacctgtcgt accgcttcac tgagggtgct cagcaactgt gcgcgttggt
     2821 ccgcgaaggc gcgcttggcc cgctgcacgc catcgatctg accttccaca acgcctacgg
     2881 ccccgacaag ccgtggttct atgatcgcgc cctctccggc ggcggatgcg tcatggatct
     2941 cggcgtgcat ctgatcgacc ttgccttgtg gatgctgccg ggcgctgcag tcgtcgacgt
     3001 gacctcgaac ctgttctcag gcgggcgacc gttgacgaac acgagcgccg aggtcgagga
     3061 tttcgccgtc gccaccctca ccctcgacac cggcgcggtg gtgcggctgg cctgctcgtg
     3121 gaacttgccg gcgggtcagg aggcggtgat ctccgcctcg gcttacggag cgcacggcgg
     3181 tggagccttg cgtaatgtga acggctcctt ctacgacttc acggccgaac gctacgtcgg
     3241 aacctcaacg gaaatcctga gtaccccgcc cgatgactgg ggcggccgcg cagccatcga
     3301 atgggcgagc cggctccggc ggggccaaag gttcgacccc gagatcgaaa gcatcgtcgc
     3361 cgtcgccgac gccatcgaca ggatctacga tcgataaccg agcccttcgg agcaaaccag
     3421 gattcgcctc cgaacgcgtc ctcgcacagg ccccgactgg attatcagcg gacggtgatc
     3481 gcgccggatc ggtcagccga gtcattgtcg tgatgtgcct gcaacagcga agtctacgtc
     3541 gtcagccaga gctgtagtcg gttgatgacc cccgacacca cttgcagggc atcgaaagga
     3601 ttgctgcccc caccttgggc atgtgtgggc gtgtcgggcc ccgaactcag gaacgtgaag
     3661 ccagatcggc ggttacacga acccggtcag cgaaatggcc ggcgccaagt cccgaccttg
     3721 cgcacgcgga ggatacatga agacgtacaa gataatcctt catcgcaaag gctgcggtgt
     3781 tcaggtcagg gtccgatgca acactgactg cagcgtcatt gcggcaacgg acgcggcgac
     3841 gacggcggct ctcctctcgt ccagcgacga cacccgttcc gtattaaggg ggactgtccg
     3901 gtcgtcggct ttcaaccccc gggaaggccg acgcccacag gctgcatgat ga
//
LOCUS       DASVTM010000172         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_549104,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000172 DASVTM010000000
VERSION     DASVTM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549104"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VF633_04770"
     CDS             <1..803
                     /locus_tag="VF633_04770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8470403.1"
                     /translation="RSVEIVILDDGTNDAQLTADLMDVIHQMVLPVRLITLTSNEGRA
                     VGRNRLAEAARGGSLLFLDSDMRPDQPRFLGTWVDLVTSQAPAVAFGGFSLLQASTEP
                     KYAVHRAMAAKSECLPWDERSKTPEKYVYTSNLLVRRDVFEAEAFDSGFSGWGWEDVE
                     WAMRVSRRYPVIHVDNPATHMGLDTTASLAGKYEQSADNFARVAAKHPDIIAGYPSFK
                     VAKMLKRLPMLKVLRPMMKQAAMADWLPVKTRAFSLRVYRAALYAEAV"
     gene            807..1751
                     /locus_tag="VF633_04775"
     CDS             807..1751
                     /locus_tag="VF633_04775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8470404.1"
                     /translation="MKDVAVIIPTMRRPESLERALKSVFAQKGVVKRLASVVVVDNDP
                     QGSAASVCGRLSVACPVPLVYRHEPRPGVSTARNAGLAATISPLIAFLDDDEKATPNW
                     LERLLKARETLNCDVVFGPIHGRVPASVGWTRRYLETFFGRQGPEQTQLLDHAYGCGN
                     SLMLRATALPGDAPFDPSTDASGGEDDALFSALAMRGGTFGWACDAWVDEYAPAHRAT
                     LKYALTRAFAYGQSPPQIAWKQGKPVRMLLWMGIGAGQAGVWALVALILTLINNDRRA
                     EALDKAARGLGKVLWTKGFEPHFYGLRELERLEKVKAG"
     gene            complement(1748..2404)
                     /locus_tag="VF633_04780"
     CDS             complement(1748..2404)
                     /locus_tag="VF633_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hfsB"
                     /protein_id="HEX8470405.1"
                     /translation="MGPGPAHRGRVIQFACAGSGEGASTVAREFARLAAVRARKPVWL
                     VDADLVQQGQQEFIAAEPDRFGRMGAAAAATPDGSVFYAVTPPVLGRDGKPVDPARLM
                     TARPCLGGRLWVTRFRAETLRSGQRAEPLPDPRYWDTMRRHADTVIIDAPAADRNDLA
                     ITLAPYVDATILVVAAEGSPDGSVGLRDEIEAVGGRIAGVVVNRSTYKPPAFLRRLVG
                     "
BASE COUNT          398 a          772 c          843 g          393 t
ORIGIN      
        1 gccggtcggt cgagatcgtg atcctcgacg acggcaccaa tgatgcgcag ctgacggcgg
       61 acctgatgga cgtaatccat cagatggtcc tgcccgtccg cctgatcacc ctcacctcta
      121 atgagggccg tgccgttggg cggaaccgtc tggccgaggc ggcgcgcggc ggaagcctgc
      181 ttttcctcga cagcgacatg cggccggacc agccccgttt tcttggaacc tgggtcgatc
      241 tggtgacatc gcaggctccg gctgtagcat tcggggggtt ctcgctgctt caggcctcga
      301 cggagccgaa gtacgcggtg catcgcgcca tggcggccaa atccgaatgc ctgccatggg
      361 acgagcgctc gaagacgccc gagaaatatg tctacacctc caacctgctg gtccgccgcg
      421 acgtgttcga ggccgaggcg ttcgattccg gcttcagcgg ctggggctgg gaggacgtgg
      481 aatgggccat gcgggtctcg cgccgctatc cggtaatcca cgtcgataat ccggcgaccc
      541 atatgggact ggacacgacg gcatcgctcg ctgggaaata cgaacagtcg gcggacaatt
      601 tcgcgcgggt cgcagccaag catccagaca tcattgcggg ctatcccagc ttcaaggtgg
      661 cgaagatgct gaagcggctg ccgatgctca aggttctgcg gccgatgatg aagcaggctg
      721 cgatggccga ctggctgccg gtcaagacgc gagcgttttc gttgcgcgtt taccgcgccg
      781 ctctctacgc cgaggcggtc taggtcatga aagacgtcgc cgtcatcatt ccgaccatgc
      841 gccgcccaga gtccctcgag cgcgcgctca agtcggtttt tgcgcagaag ggagtcgtca
      901 agcgcctcgc cagcgtggtg gtcgttgaca acgatccgca aggcagcgcg gcaagcgtct
      961 gcgggcgact gtccgtcgcc tgtcccgtcc cgctggtcta tcggcatgag ccgagaccgg
     1021 gcgtgtcgac tgcgcgcaac gccgggctgg cggcgacgat ctcacccttg atcgcgttcc
     1081 tggatgacga cgagaaggcg acgccgaact ggctggagcg attgctcaag gcgcgtgaga
     1141 ccctgaactg cgacgtggtg ttcgggccga tccatggccg cgtacccgcc agcgtcggat
     1201 ggacgcggcg gtatctggaa acgtttttcg gtcgtcaggg tcccgagcag acgcaactgc
     1261 tggatcacgc ctacggctgc ggcaactccc tgatgctgag ggcgaccgcc ctgcccggcg
     1321 atgcgccgtt cgatcccagc actgacgcca gcgggggtga ggacgacgct ctgttttcgg
     1381 cattggccat gcgcggcggc acgttcggct gggcctgcga cgcctgggtc gacgagtatg
     1441 cgcccgctca ccgcgccacc ctcaaatacg ccctcacccg cgccttcgcc tatggccaga
     1501 gcccgcccca gatcgcctgg aaacagggca agccggtacg catgctgctg tggatgggga
     1561 tcggcgcggg acaggccggg gtgtgggcgc tggtcgctct catcttgacc ctgatcaaca
     1621 acgacaggcg cgccgaggcg ctggacaagg cggccagagg tctcggcaag gtgttgtgga
     1681 ccaagggctt cgagccgcat ttctacggcc tgcgcgaact agaacggctg gagaaggtga
     1741 aggccggtta gcccaccaac cgccgcagga acgcaggcgg cttgtaggtc gagcggttga
     1801 cgacgacgcc ggcgatgcgg ccgccgacgg cttcgatctc gtcgcgcagg ccgaccgatc
     1861 cgtcgggcga gccttcggcg gctacgacca ggatcgtggc gtccacatag ggcgcgaggg
     1921 tgatggcgag gtcattgcgg tcggcggcgg gcgcgtcgat gatcaccgtg tcggcgtggc
     1981 ggcgcatggt gtcccaatag cgcgggtctg gcaggggctc ggccctctgg cccgagcgca
     2041 gcgtctcggc gcgaaaccgg gtaacccaaa ggcgaccgcc caggcagggt cgcgcggtca
     2101 tcaggcgagc cggatcgacg ggcttgccgt cacggcccag caccggcggc gtcactgcgt
     2161 agaacactga cccgtccggt gtggcggcgg cggcggcgcc catgcggccg aagcggtcag
     2221 gctcggcggc gatgaactcc tgctggccct gctgcaccag atcggcgtcg acgagccaga
     2281 cgggtttgcg ggcgcgcacg gcagcgaggc gggcgaactc acgggcgacc gtcgaggctc
     2341 cctcgcccga accggcgcag gcgaactgga tcaccctgcc gcgatgcgca ggtccgggcc
     2401 ccagtg
//
LOCUS       DASVTM010000173         7260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_974768,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000173 DASVTM010000000
VERSION     DASVTM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974768"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..78)
                     /locus_tag="VF633_04785"
     CDS             complement(<1..78)
                     /locus_tag="VF633_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX8470406.1"
                     /translation="MGQKINPIGFRLGVNRTWDSRWFADG"
     gene            complement(80..460)
                     /gene="rplV"
                     /locus_tag="VF633_04790"
     CDS             complement(80..460)
                     /gene="rplV"
                     /locus_tag="VF633_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX8470407.1"
                     /translation="MPKTNNPRRVGNTEARAKLVNVRISPQKLNLVAQSIRGLPVQKA
                     LNELEFSRKRISDDVRKVLYSAVSNAENNHNLDIDNLVVAEAFVGKNLVMKRFASRAR
                     GRSSRILKPFSEITIVVREAGEAA"
     gene            complement(462..743)
                     /gene="rpsS"
                     /locus_tag="VF633_04795"
     CDS             complement(462..743)
                     /gene="rpsS"
                     /locus_tag="VF633_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX8470408.1"
                     /translation="MARSSWKGPFVDGYLLKKADAVATSGRSKDVIKTWSRRSTILPQ
                     FVGLTFGVHNGHKHVPVHVNEDMVGMKLGEFSPTRSFPGHAADKKAKRK"
     gene            complement(746..1579)
                     /gene="rplB"
                     /locus_tag="VF633_04800"
     CDS             complement(746..1579)
                     /gene="rplB"
                     /locus_tag="VF633_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX8470409.1"
                     /translation="MALKTYNPTSPGRRALVLIDRSELFKGRPEKSLTEGLTKSGGRG
                     QGGRIAVRFRGGGAKRLYRKIDFKRRKFMTATVERLEYDPNRTAFIALITYEDGEKAY
                     IVAPQRLKAGDTIVAGEKTDVKPGNAMPLRSVPVGTIIHCVEMKPGKGAQLARSAGAY
                     AQLVGRDAGYAQIRLGSGELRMVLDGCMATVGAVSNADHMNQNLGKAGRVRHMGFRPH
                     VRGVAMNPVDHPHGGGEGRTSGGRTPVTPWGKDTKGTRTRKNKATDKYIIRTRHVKKA
                     R"
     gene            complement(1584..1886)
                     /locus_tag="VF633_04805"
     CDS             complement(1584..1886)
                     /locus_tag="VF633_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX8470410.1"
                     /translation="MAAAPTAKHYDTILSPIITEKATILSEQNKVVFRVAGTSTKDEI
                     AAAVESLFKVNVLKVNTMVTKGKTKRFRGILGRRADIKKAIVTLADGQSIDVTTGL"
     gene            complement(1886..2554)
                     /gene="rplD"
                     /locus_tag="VF633_04810"
     CDS             complement(1886..2554)
                     /gene="rplD"
                     /locus_tag="VF633_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX8470411.1"
                     /translation="MKLSVINLDGAAAGDVELSDIVFGISDIRGDILARYVNWQLAKR
                     RAGTHKVQTRNENSRTGKKMYKQKGTGGARHGSRRAPQFVGGSRAFGPIVRDHGFDLP
                     KKVRALALRHALSSKAKSGDLVVVDALTLAEVKTSALRDTFGKLGWTRTLIIAGPEVD
                     VNFALASRNIPQVHVLPNAGLNVYDILRADKLVLSKAAVDAIEATYADYKPSRREQAQ
                     KEAA"
     gene            complement(2551..3306)
                     /gene="rplC"
                     /locus_tag="VF633_04815"
     CDS             complement(2551..3306)
                     /gene="rplC"
                     /locus_tag="VF633_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX8470412.1"
                     /translation="MRTGVIAKKLGMTRLFAEDGAHIPVTILELDGCQVVGQRTQERD
                     GYVALQLGAGTKKAKNTNKAQRTTFATAEVEPKHHVTEFRVSDDALIEVGATLSADHF
                     VAGQKVDIQGETIGKGFAGGMKRWNFGGLRATHGVSLSHRSLGSTGQRQDPGKTFKGK
                     KMAGQLGMETITTQNLTVVRVDVERGLIMVKGAVPGHEGSWVKVRDAHKKPFADLPFP
                     GSFKAKGASAAPVAAETPAEDAPAAEVTEGGEA"
     gene            complement(3329..3634)
                     /gene="rpsJ"
                     /locus_tag="VF633_04820"
     CDS             complement(3329..3634)
                     /gene="rpsJ"
                     /locus_tag="VF633_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX8470413.1"
                     /translation="MDQSIRIRLKAFDHRVLDFSTREIVNTAKRTGATVRGPIPLPTL
                     IEKFTVNRSPHVDKKSREQFEIRTHKRVLDIVDPTPQTVDALMKLDLSAGVDVEIKI"
     gene            4366..5115
                     /locus_tag="VF633_04825"
     CDS             4366..5115
                     /locus_tag="VF633_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8470414.1"
                     /translation="MKTLLLATAAVSMFAAPALAQDAIGSVGVAYVNSSIDNSDLESE
                     GAVIDGNIAMPAFGAWTVTLDAAATFADSDLNDDTALRGSAHLTTMVNGDIRLGGFVG
                     ATDAGDDTLFTVGVDAQKYFAGSTLTGLVSYGSVDDIDLWTVGGDVAFYVQPNLRLNA
                     GASWNNVEANGVDADAWAYGAGAEYQFAASPFSAFATYNRVSLEDTDVDVDTFQVGVR
                     YSFGGGLQARDRSGANLGRTVLNAAGAFGGL"
     gene            complement(5215..6150)
                     /locus_tag="VF633_04830"
     CDS             complement(5215..6150)
                     /locus_tag="VF633_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX8470415.1"
                     /translation="MNFAVVATAASIAVLGFAAAAPAFASDGPEVRIRHSVARMVVVV
                     EDRADVVVEIEGGAAGLPRPTVTRIRNEIRIDGGLGRNATRNCSTGNSAGRQPGEGAS
                     VEVRGKGRVELSAAPLILVRTPRQVSVSVEGDAVFGAVGRGAQAVELSNNGCGGWTVA
                     NVAGPLSVSIAGSGDIRAGTSGKLEANIMGSGDLWAGATGDIEANIMGSGDMNLGAGN
                     RLEANIMGSGDLNVAAINGPLSANIPGSGNITVRGGQAANVSANVMGSGDIDLQGQAG
                     SVSANIMGSGDIHIARVTGAVSQSTMGSGRVHIGG"
     gene            complement(6251..7159)
                     /locus_tag="VF633_04835"
     CDS             complement(6251..7159)
                     /locus_tag="VF633_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX8470416.1"
                     /translation="MVAKRTRYPGPITEAKKHSHDLVARGFATDEALAQVLNRYPAEL
                     FDINLYDYDDEGQVTLRTGARGKLSGDKLLEAIQAGRLWVNLRDARNGCPDLWAAAMD
                     EFRKVEATYPGLKAVKNAGQLIISSPVAKVPYHFDPAGVVLFHLRGCKRVFVYPGDEQ
                     HLPERNMEQVVARQTTEELPYVRAFESDAQVMDLEPGEALTWPLYAPHRVENLDRFCV
                     SLSMDFQTWPSRFRNGALYTNAVMRSRGGSPRFTDTMSSPELAARWAASLMLKRVGGL
                     KSRLSSFERDFEPALGASDGAGALRV"
BASE COUNT         1248 a         2324 c         2293 g         1395 t
ORIGIN      
        1 accgtccgcg aaccagcggc tgtcccaggt acggttcacg ccgaggcgga aaccgatcgg
       61 attgattttc tgacccatat caggcggcct cgccggcttc acggaccacg atggtgatct
      121 cgctgaacgg tttcaggata cgcgacgagc gaccacgagc gcggctcgcg aaacgcttca
      181 tcaccaggtt tttgcccacg aaggcctcgg cgacgacgag gttgtcgatg tcgaggttgt
      241 ggttgttctc ggcgttcgac acagccgaat acagaacctt gcgaacgtcg tcggagatgc
      301 gcttgcggct gaactcaagt tcgttcagcg ccttttgcac cggcaggcca cggatggact
      361 gcgcgacgag gttcagcttc tgagggctga tgcggacgtt gaccagcttg gcgcgggcct
      421 ccgtattgcc gacgcggcgg ggattgttgg tctttggcat gttacttccg cttcgccttc
      481 ttgtccgcgg cgtggccggg gaacgaacgg gtgggcgaga actcgcccag cttcatgccg
      541 accatgtcct cgttcacgtg cacaggaacg tgcttgtgac cgttgtggac gccaaaagta
      601 aggcccacga actgcggcag gatggtggag cgacgcgacc acgtcttgat cacgtctttg
      661 ctgcggcccg acgtcgcaac ggcgtcggcc ttcttgagca gatacccgtc gacgaacggg
      721 cctttccagg aggagcgagc catctttagc gagccttctt aacgtggcgg gtacggatga
      781 tgtatttgtc cgtcgccttg ttcttgcgag tgcgcgtgcc cttggtgtcc ttaccccacg
      841 gcgtaaccgg ggtacgacca ccggaggtcc ggccttcacc accaccatgc gggtggtcga
      901 cggggttcat ggcgacgccg cgaacgtgcg gacggaagcc catgtgacga acacgaccgg
      961 ccttgccgag gttctggttc atgtggtcgg cgttggaaac ggcgcccaca gtggccatgc
     1021 agccgtccag aaccatgcga agctcgcccg aacccagacg gatctgggcg tagccggcgt
     1081 cacgaccgac gagctgggcg tatgcaccgg ccgaacgggc cagctgggcg cccttgcccg
     1141 gcttcatttc aacgcagtga atgatcgttc cgaccgggac cgagcgcagc ggcatggcgt
     1201 tgccaggctt cacgtcggtc ttttcgccag ccacgatcgt gtcacccgcc ttgaggcgtt
     1261 gcggtgcgac gatataggcc ttttcgccgt cctcatacgt gatgagagcg atgaaggcgg
     1321 tacggttcgg atcgtattcc agacgttcga ccgtagcggt catgaacttc cggcgcttga
     1381 agtcgatctt gcgatacagg cgcttggcgc cgccgccgcg gaagcggaca gcgatgcgac
     1441 cgccctgacc acgtccgcct gacttggtca ggccttcggt cagcgacttt tccggacggc
     1501 ccttgaagag ctccgaacgg tcgatcagca ccagggcgcg acggcccggc gatgtcggat
     1561 tgtatgtttt caaggccatc tgatcagagc cccgtggtga cgtcgatgga ctggccgtcg
     1621 gccagcgtca cgatcgcttt tttgatgtcg gcccgacggc caaggatgcc gcggaagcgc
     1681 ttggtcttgc ccttggtcac catggtgttg accttgagca cgttgacctt gaacaggctt
     1741 tcaacggcag cggcgatttc gtccttggtg gacgtgccgg cgacgcggaa gacgaccttg
     1801 ttctgctctg acaggatcgt cgccttttcg gtgatgatcg gcgacaggat ggtgtcgtag
     1861 tgtttggcgg tgggagcggc ggccattacg cggcttcctt ctgagcttgc tcgcgacgcg
     1921 agggcttgta gtcggcgtag gtcgcttcga tagcgtccac agccgccttg gaaagcacca
     1981 gcttgtccgc gcgcaggatg tcataaacgt tgaggccggc gttcggcagc acgtggactt
     2041 gcgggatgtt ccgcgaggcc agagcaaagt tcacgtcgac ttcaggtccg gcgatgatca
     2101 gcgtccgggt ccagcccagc ttgccgaagg tgtcgcgcag agccgaggtt ttcacttcag
     2161 ccagcgtcag ggcgtcgacg acgacgagat cgccgctctt ggccttggac gacagggcgt
     2221 gacgcagggc caaggcacgg accttcttgg ggagatcaaa gccgtgatcg cgaacgatcg
     2281 ggccgaaagc gcgcgaacca ccaacgaact gcggtgcacg gcgcgaaccg tgacgagcgc
     2341 cgccggtgcc cttttgcttg tacatcttct tgccggtgcg agagttctcg ttccgggtct
     2401 gaaccttgtg cgtaccggcg cggcgcttgg ccagttgcca gttgacgtaa cgggccagga
     2461 tgtcgccgcg gatgtcggag ataccgaaaa cgatatccga cagttccacg tcaccggcag
     2521 ccgcgccgtc gaggttgatc actgaaagtt tcatgcttca ccgccttcgg tcacttcggc
     2581 cgccggagcg tcctcagcag gggtctcagc ggcgacagga gcagccgaag cgcccttggc
     2641 cttgaaagaa cccgggaaag gcaggtcggc gaacggcttc ttgtgcgcgt cgcgaacctt
     2701 gacccacgaa ccctcgtggc cggggacagc gcccttgacc atgatgaggc cgcgctcgac
     2761 gtcgacacgg acgacggtca ggttttgcgt ggtgatggtt tccatcccca gctgaccggc
     2821 catcttcttg cccttgaacg tcttgcccgg atcctggcgt tggccagtcg aacccagcga
     2881 acggtgcgaa agcgacacgc cgtgcgtggc tcgcagaccg ccgaagttcc agcgcttcat
     2941 accaccggca aaacccttgc cgatcgtttc gccctggatg tccaccttct gaccggcgac
     3001 gaaatggtct gccgagagag tggcgccgac ttcgatcagc gcgtcgtcag acacgcggaa
     3061 ctcagtgacg tggtgcttgg gctcgacttc cgccgtagcg aaggtcgtgc gttgagcctt
     3121 gttcgtgttt tttgccttct tggtgccggc gcccagttgc agggcgacgt agccgtcccg
     3181 ctcctgagta cgttgaccca cgacctggca gccatccagc tccagaatcg tgaccgggat
     3241 atgcgcgccg tcttcagcga agagacgcgt cattcccagt tttttggcga tcacgcccgt
     3301 gcgcatatcg gatcccgcct ttgtttcttt aaatcttgat ctcgacgtcc acgccagcgg
     3361 acaggtcgag cttcatgagc gcgtccacgg tctgcggggt ggggtcgacg atgtcgagga
     3421 cacgcttgtg cgtgcggatt tcaaactgct cgcgcgactt cttatcgacg tgcggcgagc
     3481 ggttcacggt gaacttttcg atcagggtcg gcaacgggat cggaccacga actgtggcgc
     3541 ccgtgcgctt ggccgtattc acgatttcgc gcgtcgaaaa gtcgaggacg cggtgatcga
     3601 aggccttgag cctgatgcgg atgctttgat ccatatcggt ctttatatcc caagcaggcg
     3661 aacctgcgtc cctgtgaacc atttcaaaga ccggaggcct tcaggcgtaa aacgccctca
     3721 gagtacgcac gtccgcaaaa acgatcggcc cacgcagtcg cccgcgaagg ccgttgaagt
     3781 cctggttcgc tacaaagaac aatccgaggt cgttcctgcg gaccgcgtct gcgacttgcc
     3841 ccgaggggtt cgccgcacag ccggtccaac aagagttggt gcttatgacg atcgattccg
     3901 ttttcgtcaa gcggcgaaac cgtggcgcag ccgggttttg cggactctgg cagggccgtg
     3961 gcgagcaggt cacgccgccc gcgccgaacg ggttctatat actacgcgtg accggctttt
     4021 gagggtcctc tatatatacg gtgggcgaca cctgtgttgt ggacgcaagt cggcgtctgg
     4081 atagccactg aaccctcagg gcagttcgag cctccccccg acagaacgcg cttcatcggt
     4141 catcggttga ccaaatcctt gaaatccgaa gcaatattaa ccacttgcca cattcggcac
     4201 gccgcctgac gcatttgagc cgcaatgcgt gatgcttcgg ccacgcttgg cggtagtgtc
     4261 acatcattga agcaaagggc gtgcgcattg gcagccggac ctgatagcag cgccctcccc
     4321 gcgagcctcg acgcgcacgg gcatactgca atcaaggact acactatgaa aactctcctc
     4381 ctcgcgaccg ccgccgtttc gatgttcgcc gcccctgccc tggctcagga tgcaatcggt
     4441 tcggttggcg tcgcttacgt gaacagctcg atcgacaact ccgatctcga atcggaaggc
     4501 gccgtgatcg acggcaacat cgccatgccc gctttcggcg cctggaccgt gacgctggac
     4561 gccgcagcga ccttcgctga cagcgacctg aacgatgaca ccgccctgcg cggctcggct
     4621 cacctgacga ccatggtcaa cggcgacatc cgcctcggcg gcttcgtcgg cgcgaccgac
     4681 gcaggtgacg acaccctctt caccgtcggc gtcgacgccc agaaatactt cgccggttcg
     4741 accctgacgg gtctggtctc ctacggttcg gtcgacgaca tcgacctgtg gaccgtcggc
     4801 ggcgatgtcg ccttctacgt gcagcctaac ctgcgcctga acgctggcgc ctcgtggaac
     4861 aacgtcgaag ccaatggcgt ggatgctgac gcctgggctt acggcgccgg cgctgaatac
     4921 cagttcgcag cctcgccctt cagcgcgttc gcgacataca accgcgtctc gctggaagac
     4981 accgacgtcg acgtcgacac cttccaggtc ggcgttcgct acagcttcgg tggcggcctc
     5041 caggctcgtg accgttcggg cgccaacctc ggccgcaccg tgctgaacgc agccggcgct
     5101 ttcggcggcc tgtaagaccc ggtcgggcct gtcccgactg atcgaacaga agccccggcg
     5161 gagcaatccg ccggggcttt ttcgtgcgtg ttttccgagt ggcaggcgcg aggcctagcc
     5221 gccgatgtgg acgcggcccg agcccatggt gctttggctg acggcgccgg tgacgcgggc
     5281 gatgtggatg tcgcctgagc ccatgatgtt ggcggacacc gatcccgcct gcccctgcag
     5341 gtcgatgtcc cctgagccca tgacgttggc cgagacattg gcggcctgac cgccgcggac
     5401 ggtgatattg cccgaccccg ggatgttggc cgacagcgga ccgttgatcg cggcgacgtt
     5461 caggtctccg gatcccatga tgttggcttc caggcgattt ccggcgccga ggttcatgtc
     5521 gccggacccc atgatgttgg cctcgatgtc gccggtcgcc ccggcccaca gatcgcccga
     5581 gcccatgatg ttggcttcca gcttgccaga ggtcccggcg cgaatgtcgc ccgaccccgc
     5641 gatcgacacc gacagcgggc cggcgacgtt ggctacagtc cagccaccac aaccgttgtt
     5701 ggacagctcg acggcctgtg cgccccggcc aacggcgcca aacaccgcgt cgccttcgac
     5761 gctgacggac acctgacgcg gggtgcggac gaggatcagg ggcgcggcgg acagttccac
     5821 gcgacccttg cctcgtacct cgactgacgc gccctcacca ggctggcgac cggcggagtt
     5881 gccggtgctg cagttgcggg tggcgttgcg cccgaggcca ccgtcgatgc ggatttcgtt
     5941 gcggatgcgg gtcacggtcg gacgcggaag gcctgcggcg ccgccttcga tctcgacgac
     6001 gacatccgcg cggtcctcga ccaccacgac catccgggcg accgaatgcc ggatgcggac
     6061 ttcgggtcca tcgctggcga aggcgggtgc ggcggcggcg aaaccaagga cggcgatgga
     6121 ggctgcggtg gcgacaactg cgaagttcat gacgggtttc ctctccggtt ctgaccggaa
     6181 aatgctccca tgctcacgcc gagtcatttt aactcgacgt catgacaccg aattcacgaa
     6241 cgccggtctg tcagactcga agcgcgccgg ctccatccga ggcgccgagt gcgggttcga
     6301 agtcgcgctc gaagctactg agccggctct tcagcccgcc gacacgcttc aacatcagcg
     6361 acgccgccca gcgcgctgcc agctccggtg aagacatagt gtcggtgaaa cgcggcgagc
     6421 cgccccgact gcgcatcact gcgttggtat aaagggcgcc gttcctgaac cgcgacggcc
     6481 aggtctggaa gtccatcgaa aggctgacgc agaaccggtc gaggttctcg accctgtgcg
     6541 gcgcgtacag cggccaggtc agggcctcgc ccggctccag atccatgacc tgtgcatcgc
     6601 tctcgaaggc gcggacgtag ggcagttcct cggtcgtctg gcgcgcgacg acctgctcca
     6661 tgttgcgctc tgggagatgc tgctcgtcgc ccggataaac gaaaacccgc ttgcaaccac
     6721 gcaggtggaa cagcaccacc cctgccgggt cgaaatgata cggcaccttg gcgaccggcg
     6781 acgaaatgat cagctggccg gcgttcttga ccgctttcag ccccggatag gtcgcctcga
     6841 ccttcctgaa ctcatccatg gcggcggccc acagatcggg acagccgttg cgggcgtcgc
     6901 gcaggttgac ccacagccga ccggcctgaa tggcctccag cagcttgtcg cccgacagct
     6961 tgcctcgcgc cccggtccgc agcgtgacct gaccttcgtc gtcgtagtcg tacaggttga
     7021 tgtcgaacag ctcggccgga tagcgattca gcacctgcgc cagcgcctcg tcggtcgcga
     7081 acccacgcgc gaccagatcg tgcgagtgct tcttcgcctc ggtaatcggt ccgggatagc
     7141 gggtgcgttt cgcgaccacg tccctcatcc gctcaccgcc gggtcggcca tccgctgagc
     7201 cgcgcgcgcc ctgcccttgc tcatggcgac ctgggccgct ccaacagcgc ccttgaaccg
//
LOCUS       DASVTM010000174         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_17508, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000174 DASVTM010000000
VERSION     DASVTM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17508"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VF633_04840"
     CDS             complement(<1..232)
                     /locus_tag="VF633_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470417.1"
                     /translation="MNDRPTGLGPSRVIAVLGPTNTGKTHLAVERMLGHASGMIGLPL
                     RLLAREIYERIVKLRGASAVALITGEEKIIPPR"
     gene            360..917
                     /locus_tag="VF633_04845"
     CDS             360..917
                     /locus_tag="VF633_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470418.1"
                     /translation="MRRYSMTTVTMVAAMGLTAAAAGPASALTLQDAPATVVRPGDDT
                     LSCRQMADEGAALSADMGESGPGLLGRVGGIARAGAALLVPGAGLAVAGADALTSPAK
                     NRREAEADTRRDRWNYLNGLYAGRGCDQGDARTSPVSQTPAVPPPASVVPGSASGPAP
                     TASTTPSRAPASIPAPAITPASFPH"
     gene            complement(946..1749)
                     /locus_tag="VF633_04850"
     CDS             complement(946..1749)
                     /locus_tag="VF633_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8470419.1"
                     /translation="MIGSLFRRAKPASAPASVPDGAVVWAIGDVHGRADLLGPLLKAA
                     VEDLRTTRPVRPVIVMLGDYVDRGEDSRGVIDLLCDLANQGDIETCFLRGNHEDRFEA
                     FLSQPELGPAWSEYGGREALRSYGVTVPALRNDPEGWAEASAQLNVALPDRHRRFLLQ
                     QRSSFEIGDYFFAHAGARPGVALADQSADDLMWIRGDFLAAKTRFEKVVVHGHTPEES
                     VHSDQRRIGIDTGAYATGVLTGLRLEANRREVCQTRLMGRTVEIIRRSL"
     gene            2056..2132
                     /locus_tag="VF633_04855"
     tRNA            2056..2132
                     /locus_tag="VF633_04855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2090..2092,aa:Arg,seq:cct)
     gene            2721..3749
                     /locus_tag="VF633_04860"
     CDS             2721..3749
                     /locus_tag="VF633_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8470420.1"
                     /translation="MTKLLFDRGPEHEPILWSTRPRPDRERWRSVLTILAICMLLALL
                     MPGIAALTHIDHRWVDILAQFPAPALAATLLLVIASAALRLWPAMITGALVALLLAVA
                     VWPQAFADRGVARPGAPVVTLYSANLFFENPDASRIRASIEDANPDIIVLVETSRQVI
                     ERLDGVLAGYPYRIVASEDSLGRTDRTVIASRYPVRREGQIGSAVTYVRAVVDTPLGP
                     LQVIGAHLTRPWPYQIEWEQIRQALAVKSLLVEAKGPTVVAGDFNSVSSARIGRLLRK
                     EAGLRANPGWPGTWPARLPAVAGITIDQVYRTPDLAVVSRRVGQRTGSDHRPVVTKLT
                     LSEVASKG"
     gene            3842..>5397
                     /locus_tag="VF633_04865"
     CDS             3842..>5397
                     /locus_tag="VF633_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470421.1"
                     /translation="MTTGFIRREVLGGLLSTPALSGLISSATEAIDTTAAIATPVLLT
                     DFRRDSYLVNGRRRGDASSTPGLAARHAGGLALTPEGALRAFPAGMPRQDDKAGLLLE
                     AASGNLSAGYNAAPMTTLGWSASPGVGSATWTVVDRRDELYAATAPASGALIFRDLID
                     AGIMNGNVLQGYNPDPRKPFVVLADGAIATAGAHSISAWVRCVSGTGDIRISGAANGP
                     KFGHTDWRRTIRANITAAVGKGLSVVVDPMSTVEIILWQIEPGPMCTSPIVVAGTPAV
                     RAADRLSLVNSSLFKAPFTVVMDAFMDRADAVDRTWFSVSGSGAAVTVTRARDNALIL
                     SGSGEGHDGTLQPRVPRILGPGGARTALQVRAQGRSVSVASANAHDAFSLAPEGLDTL
                     MIANGPDGSTPLNAWVRSVEISGIGTAAALAVATSPPVDAMAHDIRRYVDPAGDDSAD
                     GLTPATAWATLARVAASTLPAGIQVLLKRGASWSETVSPPSHWCTIGTYGEGSRPVVG
                     AGQDYGFDENG"
BASE COUNT          963 a         1799 c         1724 g          911 t
ORIGIN      
        1 gccgcggcgg gatgatcttt tcctcgccgg tgatcagggc gaccgccgac gccccacgca
       61 gctttacgat ccgctcatag atctcgcgcg ccagcagacg cagcggcagg ccgatcatgc
      121 ccgaggcgtg acccagcatc cgttcgaccg ccagatgggt cttgccggta ttcgtcggcc
      181 ccagcaccgc gataacgcgg gagggcccca gacctgtcgg gcggtcattc atgatttgca
      241 taaggtggcg attagatcgc ggttcctcaa gcggaacagt tccgacttca agccagccaa
      301 ggcaacggat cgcaaacgcg cgtcgttaag tgcctgacgg ggaagaacgg gagtccgaaa
      361 tgcgacgcta ttcgatgacg acggtgacca tggtcgcggc gatgggattg acggcggcgg
      421 cggcgggccc cgcatcggcc ctcacgctgc aagacgcgcc ggcgacggtg gttcgccccg
      481 gcgacgacac cctgtcctgc cgccagatgg ctgatgaggg cgccgccctc agcgccgaca
      541 tgggcgaatc cggtccggga cttctgggcc gcgtcggcgg tatcgctcgc gcgggcgccg
      601 cactgctggt gcccggcgcg gggctggccg ttgcgggcgc cgacgccctg accagcccgg
      661 cgaaaaaccg gcgggaagcc gaggccgaca cccgccgcga ccgctggaac tatctgaatg
      721 gtctctatgc cggtcggggc tgcgatcagg gggacgcgcg gacatcacct gtgtcacaga
      781 cgccggccgt ccctccgcca gccagtgtcg ttcctggatc tgcctccgga ccagccccga
      841 cagcttctac gacgccaagc cgggcgccgg cttcgattcc ggcgccggcc atcaccccgg
      901 cgtctttccc gcactgaggc agcgacggag cctcgcgcac gcctgtcaaa gagaccggcg
      961 tattatttcg acagtccggc ccatcagccg ggtctggcat acctcgcgcc ggttggcctc
     1021 aagacgcaga ccggtcagca cgccggtggc ataggccccg gtgtctatgc cgatgcggcg
     1081 ctgatcggaa tggaccgact cttccggcgt atgcccgtgc accacgacct tctcaaaacg
     1141 ggtcttggcc gccagaaaat caccccggat ccacataaga tcatccgccg actggtcggc
     1201 cagtgcgacg ccgggccgcg ccccggcgtg ggcaaagaaa taatccccga tctcgaagga
     1261 cgacctctgc tgcagcagga aacgccggtg ccgatccggg agggcgacgt tcagctgggc
     1321 cgacgcctcc gcccagcctt cggggtcgtt gcgcagggcc ggaacagtga caccgtacga
     1381 acgcaacgcc tcgcgcccgc catactcgct ccaggctgga ccgagctcgg gctggctcag
     1441 aaaggcttcg aagcggtctt catgattgcc gcgcaggaaa caggtctcga tgtccccctg
     1501 attggccaga tcgcacagaa ggtcgatcac gccacgggag tcctcgccgc ggtccacata
     1561 gtcgcccagc atcacgatca cagggcgtac aggcctggtc gttcgcaggt cttccacagc
     1621 cgccttcaga agcgggccca gcagatcagc ccggccatgc acgtcaccga tcgcccagac
     1681 tacagctccg tcgggaacgg acgcgggagc gctggcgggt ttggcgcggc gaaacaggga
     1741 tccgatcatt cgctccacat cggagcattc cgcttccaga acaaggcttg tacccctcag
     1801 ccccgcaaaa agcgtcgccg ggaggcttca tgcccttaag gccattaatg ccggcgaccg
     1861 ttcagcggaa cagggaagaa acaaagcggg accgaatcag cgacaccttc gtattcaggc
     1921 tttgttccgt acaacatatt gtatcttaaa ggttgttaac cacaatcggg tcgttggaag
     1981 gccgcatcat tgatgggagc ttcgaagaat ggggacttcc cccaaccaca tcaaaccgct
     2041 aggccaccca ggcgtgaccc cgtagctcag ccggatagag caaggctttc ctaaagcaga
     2101 ggtcgggggt tcgagtccct ccggggtcgc catttcgctg ttcttgctgg taggaaatcc
     2161 gacccgcagc cagcctgtgc gagaccacct ggcggtctag cgcgctgatc gccctggtcc
     2221 gccaccccga gaaagccggg cgacgggctt gcgcagcatc ctgtatctct gaacgatccg
     2281 ccaatgccgg acgctgctcg cgccagccgt cctggtcgag cctccacgat atcaatagcg
     2341 gcgacgacga cctcgtcttc tgaggcgctt tagctggtcg ttcaagccga ggggttgcgc
     2401 caggaaaagc caggccagcc ataaaccaag cagcaggaga atcccgaagg agagcataat
     2461 catgagtgtc gccagagacg gggggagcgg ttcggacgtt gtcagactgc cacagccgcg
     2521 aggcgcgagc agcccgttgc ttggacccgg agaaagcggc gtgagaaaga ccggaaggga
     2581 aaacctccat cctccggcca ggtttcacct cgacatcgga atcgctcttc atgttacctt
     2641 gactgccagg ccgccttccc tccctccctc ggccgacggc tccacgctca ctgtcaccgc
     2701 cttcaccgga gcctcgcgcc atgaccaagc tgctgttcga tcgcgggcca gaacacgagc
     2761 cgatcctctg gtcgactcgc ccgcggccgg atcgcgagag atggcgatcc gtcctcacga
     2821 tactggcgat ttgcatgctg ctcgctctgc tcatgcccgg cattgcagcg ctcactcaca
     2881 ttgatcatcg ctgggtcgat attctggctc agttccctgc gcctgccctc gcggccacgc
     2941 tgctgcttgt gatcgcttca gcagcgctca ggctttggcc ggcgatgatt accggggcgc
     3001 tcgtagcgct gctgctcgct gtcgccgtct ggccgcaagc ctttgccgat cgaggcgtgg
     3061 cgcgccccgg agcgccggtg gtgaccctct attccgcaaa tctgtttttc gagaacccgg
     3121 acgcgtccag gatccgggcg tcgatcgagg atgctaaccc ggacatcata gtgctggtgg
     3181 agacgtcgcg gcaggtcatc gagcgactcg acggggtgct ggccggatac ccttaccgca
     3241 tagtggcgtc tgaagactcg ctcgggcgaa ccgaccggac cgtcatagcc tcgcgatatc
     3301 cagtccggag agagggccag attggcagtg ctgtgaccta tgtccgcgcg gtggtcgata
     3361 cgccgctggg tcccctccag gtcatcggtg ctcatctgac gcgcccatgg ccctaccaga
     3421 ttgaatggga gcagatcagg caggccctcg ccgtgaaatc cttgctcgta gaagcgaagg
     3481 gtcccaccgt cgtcgctggc gacttcaaca gcgtctcgag cgcgcggatc ggtcggctgc
     3541 tgcgcaagga ggccggcttg agggccaatc cgggatggcc cggaacctgg ccggcgcgac
     3601 tcccggccgt ggccggcatc acgatcgatc aggtctatcg cacgcctgat ctcgcggtcg
     3661 tcagccgccg cgtcgggcag aggaccggtt cggaccatcg ccctgtcgtc acgaagctga
     3721 ccctctcgga agtggcatcc aagggctgat ccgggcgttc tgacagacac cttctgcgat
     3781 cctcccccgg gccgcgactg atatcgtgca agtgcgggct tcaactcgag ggatatgagc
     3841 gatgaccacc ggcttcatca gacgtgaggt cctcggcgga cttctgtcga ccccggcgct
     3901 gagcggcctg atctcttctg cgacagaggc gatcgacacg acggcagcca tcgccacgcc
     3961 ggttcttctt accgattttc gacgagacag ttatctggtc aacggcagga gacgggggga
     4021 cgcctcgtcc acgccgggac tggcggcccg ccacgcgggc gggctggcgc tgacgcctga
     4081 gggcgccttg cgggcttttc cagcgggtat gccgcgacag gacgacaagg cgggcctgct
     4141 gctggaagcc gcatcaggaa atctgtccgc aggctacaac gcggccccga tgacaacgct
     4201 gggctggagc gcaagccccg gggtgggaag cgcaacctgg accgtcgtcg atcgccggga
     4261 tgagctttac gcggccaccg cccccgccag cggcgcgctc atttttcgcg acctgatcga
     4321 tgccgggatc atgaacggca acgtgcttca gggatacaat ccggacccga ggaagccttt
     4381 tgtcgttttg gccgacggcg ctatcgcaac agcgggcgca cattcgattt ccgcctgggt
     4441 ccggtgcgtg tccggaaccg gggacatccg gatcagcggg gcggccaacg gaccgaaatt
     4501 cggtcacacg gattggcgtc gaacaatccg ggccaacatc accgccgctg tgggcaaggg
     4561 cctgtcggtg gtggtggacc cgatgtccac agtcgaaatc atcctgtggc aaatcgagcc
     4621 cgggccaatg tgcaccagcc ccatcgtcgt cgcaggcact ccggcggtgc gggcggctga
     4681 ccggctgtcc ctggtgaact ccagcctttt caaggcgccg ttcaccgtcg tgatggatgc
     4741 gttcatggac cgcgccgacg ccgtcgaccg cacatggttt tccgtgtcgg gctccggggc
     4801 ggcggtcacc gtcacccggg cccgggacaa cgccctgatc ctctcgggat caggcgaagg
     4861 ccacgacggg acactgcaac cccgtgtccc ccgtattctc ggccccggcg gcgcgcgaac
     4921 ggcgctgcag gtcagagccc agggtcgcag tgtttccgtc gcgagcgcca acgcccacga
     4981 tgctttcagc ctcgcgccag agggcctcga taccctgatg atcgcgaacg gcccggacgg
     5041 ctccacaccc ctcaacgcgt gggttcgaag cgtcgagatt tccggcattg gcacggctgc
     5101 ggcgctggcg gttgctacga gccctcccgt ggacgcaatg gcgcatgaca ttcgccggta
     5161 tgtcgacccc gcgggcgacg atagcgcaga tggcctgacg cctgcgacgg cctgggcgac
     5221 cctggcgagg gtcgcagcca gcacactacc ggccggcatc caggtgctgc tcaagcgcgg
     5281 agcgtcatgg tcggagaccg tgtcgccgcc ctcccactgg tgcacgatcg ggacctacgg
     5341 agaaggttcg cgaccggtag tcggcgcagg gcaggactac gggttcgacg aaaacgg
//
LOCUS       DASVTM010000175         6170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_710792,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000175 DASVTM010000000
VERSION     DASVTM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710792"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VF633_04870"
     CDS             complement(<1..469)
                     /locus_tag="VF633_04870"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX8470422.1"
                     /translation="MSGPSSGVRVWITRTAPGAQRLADRVREAGFDPIVSPLLMTDPD
                     FPTPDLTSLLDDIAALAFTSVNGLRFADLTPRRDWPVFAVGERTAEAARARGFTDVIS
                     ADGDARTLAETIAAVWGDRPGLLLAPTAERPAADLAALLGDRAPVRSLPVYRTV"
     gene            complement(472..1449)
                     /gene="hemC"
                     /locus_tag="VF633_04875"
     CDS             complement(472..1449)
                     /gene="hemC"
                     /locus_tag="VF633_04875"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX8470423.1"
                     /translation="MTSVSPVIRIGTRRSRLALAQSGMMQRAIAAAMDLTLEQVVLVE
                     IVTTGDRIQDRRLLEVGGKALFTKEIEEALFDGRVDVAVHSMKDVPAEQPAGLLIAAI
                     PEREDATDAFISHQYASFDDLPVGAKFGTASLRRQAQALALRPDLDIQMLRGNVDTRL
                     RREAEGEFDAILLATAGLNRLGLSEVIRERLSLDRFLPAPGQGALAIQIRDGDEEAEK
                     HARWIAALNHPMTALAIAGERGAMTALEGSCRTAVGAHATFGDGHMMLTTEMLSPDGS
                     ARWRRSGVLGDIHAANAEASARILGETLGHEVHLAAGDQEVDLATLDQG"
     gene            1490..2644
                     /gene="tsaD"
                     /locus_tag="VF633_04880"
     CDS             1490..2644
                     /gene="tsaD"
                     /locus_tag="VF633_04880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX8470424.1"
                     /translation="MTIGADYSSVTGPATGGELPKGGPTGDLVVLGLETSCDETAASV
                     VRLTPDGTATVLSSVVHSQIDDHAAYGGVVPEIAARSHVEMIAGVTQRAMADAGLGYG
                     QLDGVAATAGPGLVGGVMVGLSFGKAAALARGLPFVAVNHLEGHAVSARLGAKVAYPF
                     LLLLVSGGHCQLLEVRGIGDMSRLGTTIDDAAGEAFDKIAKAMGLGYPGGPALEALAA
                     SGDGSRFDLPRALLGRKDCDFSFSGLKTAASRLAQTCETDQDRADLADAVQSAIARQL
                     SERSERAMRDYAEKHEHRLFVVAGGVAANKTVRSTLEATAAKHGFSFLAPPMAYCTDN
                     AAMIALAGAERLQLGLTSDIDTAARPRWPLDEARALADPVHPAGRKGAKA"
     gene            2654..3652
                     /locus_tag="VF633_04885"
     CDS             2654..3652
                     /locus_tag="VF633_04885"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX8470425.1"
                     /translation="MEFRSAGVIGAGAWGTALAQVTGWAGLDTLLQAREPEVVESIRE
                     RRINEAFLPGVVLDDHVNVTTEIADLASCDVILAVPPAQHLRGVMTAFAPYYRPGVPI
                     VLCAKGIERGSLKLMTEVLAESIPGATVAVLSGPSFAADVSRGLPTAVTLACADAALG
                     EELMWTLSAPGFRPYLATDLIGAEAGGALKNVLAIACGIVEGRGLGKSAHAALITRGF
                     AEMTRLAVALGGEAETVAGLCGLGDLVLTCSSPQSRNMSLGLALGQGQTVEQALAGKR
                     SVAEGYESAPAVRDLARKLGVETPICEAVAAVLAGETTVDIVIDGLLSRPLKVERN"
     gene            3649..4041
                     /locus_tag="VF633_04890"
     CDS             3649..4041
                     /locus_tag="VF633_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX8470426.1"
                     /translation="MSEEAAASEVPAERKRVWTTPANVTLGLIDDIAEPGSRGYVLQI
                     GEAYFHGFVVRKDGEVAGWVDRCPHAGFPIAIQMDRYLTPDGHLILCGWHGAVFEPIS
                     GKCVGGPCAGGKLTPWPVKVESGILRTA"
     gene            complement(4127..4276)
                     /locus_tag="VF633_04895"
     CDS             complement(4127..4276)
                     /locus_tag="VF633_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470427.1"
                     /translation="MADPKDNTDPHPGMMGDGTEEQNLGQPGDPSKRITEDEVDEAFG
                     GDEKG"
     gene            complement(4367..4876)
                     /locus_tag="VF633_04900"
     CDS             complement(4367..4876)
                     /locus_tag="VF633_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX8470428.1"
                     /translation="MTQTQLDKFLADSRRATRNARSAATTAVAGGEMMKAAGNVIAAR
                     LEILAAGMVDPGKTDLTEIGLMSSEKSEALSEAAASLSKNLGDVGERLSKSAMDEFGL
                     AGKAATEMAAAATPQAFVAAQYDYAMGWWGRATGQMLTLNTELLKAQSEALKPIHDTA
                     MANARRLKK"
     gene            complement(4903..5664)
                     /locus_tag="VF633_04905"
     CDS             complement(4903..5664)
                     /locus_tag="VF633_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX8470429.1"
                     /translation="MLIHLSSWPEIDARLKQTQPLAKTIVIPIGSNEQHGPTGLLGTD
                     WLCPEIIAHAAEKTDDSLIIAPTFNIGMAQHHLAFAGTISLRPSTFMAAITDWVSSLS
                     RHGFERIYFLNGHGGNVATIEATFSEIYAEWSFVEEQPPFVLKLKNWWDLPGVMGLCN
                     SLFPTGHGMHATPSEIAVTQAAYPDRIKTADYSPKIAPSGPIRDALDYRARFPDGRIG
                     SDPAQASPAKGQQIIDAAIPALLRDVAAFSNETLP"
     gene            5789..>6170
                     /locus_tag="VF633_04910"
     CDS             5789..>6170
                     /locus_tag="VF633_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8470430.1"
                     /translation="MTHSSAPLPSEIDVCVIGAGSAGIAAARRLIRAGVSVIVLEARE
                     RTGGRAHTIQRRLVQHRGGSLEMGLDMGCGWLHSGDENLLTEIGCDAGFTIDETPPPW
                     NQPPFAGATDDRAAFGEAFDAFDQR"
BASE COUNT          941 a         1912 c         2271 g         1046 t
ORIGIN      
        1 cgacggttcg gtagacgggg agagatcgaa ccggcgcgcg gtcgccgagc aatgccgcca
       61 gatccgctgc cgggcgttcg gcggtcgggg ccagaaggag gccgggccgg tcgccccaga
      121 ctgccgcgat ggtctcggcc aaggtgcggg cgtcgccgtc ggcggatatg acgtcggtga
      181 agcctctggc ccgcgccgcc tcggccgtgc gttcgccgac ggcgaagacg ggccagtcgc
      241 ggcggggggt caggtcggcg aaccggaggc cgttgacgct ggtgaaggcg agggccgcga
      301 tgtcatccag cagggacgtc aaatccggcg tggggaaatc tggatcggtc atcagaagcg
      361 gcgagacgat cggatcgaac ccggcctcac gaacgcggtc ggcgaggcgt tgggccccgg
      421 gcgccgtgcg ggtgatccag accctgacgc ccgagctggg cccggacacg atcagccctg
      481 atccagcgtg gcgaggtcga cttcctgatc gcctgcagcg aggtgaacct cgtggccgag
      541 cgtttcgccc aggatgcggg cggaggcttc ggcattggcg gcatggatgt cgcccagcac
      601 acccgaccgg cgccagcggg ccgagccgtc ggggctgagc atttcggtag tcagcatcat
      661 atggccgtcg ccgaaggtgg cgtgggcgcc gacggccgtg cggcacgacc cttcgagcgc
      721 ggtcatggcg ccgcgctcgc cagcgatggc caaagccgtc atcgggtggt tcagggcggc
      781 gatccagcgg gcgtgtttct cggcttcctc gtcgccgtcg cggatctgga tggcgagagc
      841 gccctgcccc ggcgcgggca aaaaccggtc gagcgacagg cgttcgcgga tgacttcgga
      901 caggcccagc cggttgagcc cggcggtggc cagcaggatg gcgtcgaact cgccctcggc
      961 ttcacgtcgc aggcgtgtgt cgacgttgcc gcgcagcatc tggatgtcga gatcggggcg
     1021 gagcgccagc gcctgggctt ggcggcgcag ggaggccgtg ccgaacttcg cgcccacagg
     1081 aaggtcgtcg aacgacgcgt attggtggct gatgaaggcg tcggtggcgt cttcgcgctc
     1141 ggggatggcg gcgatgagca ggccggcagg ctgttcggcg gggacgtcct tcatcgaatg
     1201 gacggcgacg tcgacgcggc cgtcgaacag ggcttcctcg atctccttgg tgaacagggc
     1261 cttgccgccg acttcgagca ggcggcggtc ctgaatccgg tcgccggtgg tgacgatctc
     1321 gaccagcacc acctgctcga gcgtcaggtc catggcggcg gcgatggcgc gctgcatcat
     1381 gccggactgg gccagagcca gcctggaacg ccgggtgccg atgcgaatga cgggagacac
     1441 tgaggtcatg ggcgttgcgc gggccttttg gggtcgctac ctcgaccgga tgactatcgg
     1501 cgcggactat agcagcgtga caggcccggc aaccggaggc gaattgccca agggcggacc
     1561 cacgggcgat ctggtcgttc tcgggctgga aaccagctgt gacgagacgg cggcctcggt
     1621 cgtacggctg acgcccgacg ggaccgcgac cgtcctgtcc tcggtggtgc acagccagat
     1681 cgacgaccat gcggcctatg gcggggtggt gccggaaatc gcggcgcgca gccatgtcga
     1741 gatgatcgcg ggcgtgacgc aacgcgcgat ggcggacgcc ggtctcggct atggtcagct
     1801 ggacggcgtt gcggcgacgg cggggcccgg gctggtcggc ggggtcatgg tgggcttaag
     1861 cttcggcaag gcggcggcgc tggcgcgggg cctgccgttc gtggcggtga accatctgga
     1921 gggccatgcg gtgtcggccc gactgggtgc gaaggtcgca taccccttcc tgctgctgct
     1981 ggtctcgggc gggcattgcc agttgctgga ggtgcgcggc atcggcgaca tgagtcgtct
     2041 gggcacgacg atcgacgacg ctgcgggtga ggctttcgac aagatcgcca aggccatggg
     2101 gctcggctat cccgggggtc cggcgctgga ggcattggcg gcgtcgggcg acggttcgcg
     2161 attcgacctg ccgcgcgcgc tgctgggccg caaggactgc gacttttcct tctcagggct
     2221 caagaccgct gcgtcccggc tggcccagac ctgcgagacc gatcaggatc gcgctgatct
     2281 ggccgatgcg gtgcagtccg ctatcgcacg tcagctgtcc gaacgctcgg aacgagccat
     2341 gcgggattat gcggagaagc acgagcaccg gctgttcgtg gtcgccgggg gcgtggcggc
     2401 gaacaagacg gtgcggtcga cgctggaagc aactgcggca aagcatggct tctcgttcct
     2461 cgcgccgccg atggcctatt gcaccgacaa tgccgcgatg atcgcgctgg cgggggccga
     2521 acggctgcaa ctggggctga cctcggacat tgataccgcc gcaaggccgc gctggccgct
     2581 ggacgaagcg cgcgccttgg ccgatcctgt ccaccccgcg ggccgcaagg gcgcgaaagc
     2641 ttaagggcga cgtatggaat ttcgatcggc gggcgtgatc ggcgcgggcg catggggcac
     2701 ggccctggcg caggtgaccg gctgggcggg gctcgatacc ctgttgcagg cgcgtgagcc
     2761 cgaggtggtc gaaagcatcc gcgagcgacg catcaacgag gccttcctgc ccggcgtcgt
     2821 gctggacgat cacgtcaatg tgacgacgga gatcgccgat ctggcgtcgt gcgacgtgat
     2881 cctcgccgtg ccgccggccc agcatctgcg cggcgtgatg accgccttcg cgccctatta
     2941 ccgaccgggc gtgccgatcg tgctgtgcgc caagggcatc gagcgcggct cgctgaagct
     3001 gatgaccgag gtgctggccg agtcgatccc gggcgcgacc gtcgccgtcc tgtcaggccc
     3061 aagttttgcg gccgatgttt cgcgtggcct gccgaccgcc gtcaccctcg cctgcgccga
     3121 tgcagctctg ggcgaggagt tgatgtggac gctgtcggcg cccggcttcc ggccctatct
     3181 ggcgaccgac ctgatcggcg cggaggccgg tggggcgttg aagaacgtgc tggccatcgc
     3241 ctgcggcatc gtcgagggcc ggggcctggg caagagcgct cacgccgctc tgatcactcg
     3301 ggggttcgcc gagatgacgc ggctggccgt ggcgctgggc ggtgaggcgg aaacggttgc
     3361 gggcctgtgc ggtctcggcg atctggtgct gacctgctcg agcccgcagt cgcgcaacat
     3421 gagcctgggt ctcgccctcg gtcagggaca gacggtcgaa caggcgctgg ccggtaaacg
     3481 gtcggtcgcc gagggctatg agagcgcacc ggccgtacgc gatctggcgc gaaagctcgg
     3541 ggtcgagaca ccgatctgtg aagccgtggc cgcggtgctg gcgggcgaga ccaccgtcga
     3601 catcgtcatc gacggactgc tgtcgcgccc gctcaaggtc gaacggaatt gagcgaagag
     3661 gctgccgcga gcgaggtccc cgctgagcga aagcgcgtct ggaccacccc ggcgaacgtc
     3721 acactcggcc tgatcgacga catcgcagag ccgggctcgc gcggatatgt gctgcagatc
     3781 ggcgaagcct atttccacgg gttcgtggtg cgcaaggatg gcgaagtggc cggttgggtc
     3841 gaccgctgcc cccatgccgg gtttccgatc gccatccaga tggaccgcta tctgacgccg
     3901 gacggccacc tgatcctgtg cggatggcac ggcgcggtgt ttgagccgat cagcggcaaa
     3961 tgcgtcggcg ggccatgcgc agggggcaag ctgaccccct ggccggtcaa ggtcgaaagc
     4021 gggatcctgc gtacggcgta aacggatctc aggcgtcgta tgcgcccaag accccctccg
     4081 tcacggcgaa aagagcgccg tgacacctcc cccgaaaggg gaggatttaa cccttttcgt
     4141 cgccgccgaa agcttcgtcg acttcgtctt cggtgatgcg cttggacgga tcgcccggct
     4201 ggccgaggtt ctgttcctcg gtgccgtcac ccatcatgcc gggatgcgga tcggtattgt
     4261 ccttgggatc ggccatggtc atcgtccttg ttgaagagag tggaacaacg tcggggcgga
     4321 ccccgccatg ccggaacgca aatgactagt tcttgaggcc ccgtgactat ttcttgaggc
     4381 ggcgggcgtt ggccatggcg gtgtcgtgga tgggcttcag cgcctcggac tgcgccttca
     4441 gaagttcggt gttcaaggtc agcatctgac ctgtagcccg gccccaccac cccatggcgt
     4501 agtcgtactg ggcggcgacg aaggcctgcg gcgtggcggc ggcggccatt tccgtggcgg
     4561 cttttccggc caggccgaac tcgtccatgg cggatttcga caggcgttcg ccgacatcgc
     4621 cgagattctt gctcagcgag gcggcggctt cggacagggc ctcgcttttc tcggagctca
     4681 tcagcccgat ttctgtcagg tcggtcttgc cgggatcgac catgccggcg gcgaggattt
     4741 ccagccgggc ggcgatgacg ttgcccgccg ccttcatcat ctctccgccc gcgacagccg
     4801 tggtcgccgc cgagcgggcg ttgcgcgtcg cgcgccggct gtcagcgagg aatttgtcga
     4861 gctgggtctg ggtcatggtt cagtcgtgcg cccgacactg tctcagggca aggtttcgtt
     4921 cgaaaacgcc gccacgtctc tgagcagggc cgggatggcg gcgtcgatga tctgctggcc
     4981 cttcgccggg ctggcctgcg ctggatcgga gccgatacgg ccgtcgggga agcgagcgcg
     5041 atagtcgagc gcgtcgcgga tcggaccgct cggcgcgatc tttggcgaat agtccgccgt
     5101 cttgatccgg tcgggatagg cggcctgcgt cacggcgatc tcggacgggg tggcgtgcat
     5161 gccgtgaccg gtggggaaca gcgagttgca cagccccatg acgccgggca ggtcccacca
     5221 gttcttgagc ttgagcacga acggcggctg ctcctcgaca aacgaccact ccgcatagat
     5281 ctccgagaag gtcgcctcga tggtcgcgac gttgccgccg tggccgttga ggaaatagat
     5341 ccgctcgaag ccgtgacggc tcagcgacga gacccagtcg gtgatcgcgg ccatgaaggt
     5401 cgaaggccgc agcgagatcg tcccggcgaa ggccagatgg tgctgggcca tgccgatgtt
     5461 gaaggtcggg gcgatgatca ggctgtcgtc ggtcttctcc gccgcatggg cgatgatctc
     5521 ggggcagagc cagtcggtgc cgagcaggcc ggtcggtccg tgttgctcgt tcgagccgat
     5581 gggaatgacg atggtcttgg ccaatggctg ggtctgcttc agccgcgcgt cgatctcggg
     5641 ccaggacgac agatggatca acatgaggaa ctcctacgcg gcgggacaga cgcttcttat
     5701 atcagagcga ggccgtgcgt ccggccctat tgccgcgatc ggcggttggc ggggatcgaa
     5761 ccgtcctcta tcgcgatgaa ccattgccat gacccattcc tccgctcccc tgccttctga
     5821 aatcgatgtc tgcgtgatcg gcgccgggtc cgccgggatc gccgccgcgc gcaggctgat
     5881 aagggcgggc gtttcggtca tcgtgctgga ggctcgcgaa cggaccggag gacgggcgca
     5941 caccatccag cgccgtctgg tccagcatcg cggcggcagt ctcgagatgg ggctggatat
     6001 gggctgcggc tggctgcact ctggcgacga gaacctgctt accgaaatcg gttgcgacgc
     6061 gggctttacg atcgacgaga ccccgccgcc gtggaaccag ccgcctttcg ccggggccac
     6121 ggatgacagg gccgccttcg gggaggcgtt cgacgccttc gatcaacggg
//
LOCUS       DASVTM010000176         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_388619,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000176 DASVTM010000000
VERSION     DASVTM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388619"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VF633_04915"
     CDS             complement(<1..471)
                     /locus_tag="VF633_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX8470431.1"
                     /translation="MSQVIPAEWAPHRAMWVGWPSHAELWGEDFEQAQSEVEGLVRAL
                     AGPGREQVKLMIGNDEALADAQARFADVEGVTVVSGRFGDIWLRDTGPIFGVNSKTGA
                     AFGFNGWGGKYVYQHDTEVADQIGEAAGVPLTRHVAILEGGSLDHDGLGTILTTR"
     gene            complement(571..2733)
                     /locus_tag="VF633_04920"
     CDS             complement(571..2733)
                     /locus_tag="VF633_04920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX8470432.1"
                     /translation="MKVASVLIPLPVPEAFDYEVAHDMAVERGDQVAVPLGPRLMRGI
                     VSEVRETTGSNRRLKAVETVLDDPRLPPGTVDFIEWAARWTLSAPGEMAATALKGLRA
                     PRPRPERRVCRVEGREPARPTASRTAVLEALGVRSIPGPDLARAAGVSSGVVKGLVDE
                     GVLEIVEIEAIAAFDAPDPDHAPASLNADQAASATAIAEAIDKGGFAPFLLDGVTGSG
                     KTEAYLEAAARALRADPTAQILILLPEIALTQALIARLTDRFGAAPAEWHSGIAPPRR
                     RQVWEAVVAGRCNIVVGARSALFLPFPNLKLIVVDEEHDGSFKQEEGLVYHGRDLAVA
                     RARIEGAAVVLASATPSLETLLNAHQGRYRWLKLGARHGAAVMPDIRLLDLRQNPPDP
                     QTWLSQPLREAIGETLLRREQTLLFLNRRGYAPVVLCRACGHRLTAPDTDSWLVEHRY
                     TGRLVCHLTGFSMVRPKVCPSCGASDSLVAVGPGVERVEEEVKALFPQARTAVFSSDT
                     VPDAKAARAMIQSMVDGDIDILVATQAAAKGHNFPKLTLVGVVDADLGLRGGDLRAAE
                     RTFQLLTQATGRAGRADIPGRALLQTWTPEHPVLRALAAGDRDGFVEAEMAEREAASL
                     PPYGRLAALILSGENAVAVENTADALAQAIPNAERLEVYGPADAPLALIRGRRRKRLL
                     VRADRDVDLQGFLRAWLARVKIPGSVRLTIDVDPYSFL"
     gene            2764..3417
                     /gene="fsa"
                     /locus_tag="VF633_04925"
     CDS             2764..3417
                     /gene="fsa"
                     /locus_tag="VF633_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX8470433.1"
                     /translation="MKLFLDTADVAIIKDMLPTGMVDGVTTNPSLIAKSGRNIAEVIA
                     EICALVEGPISAEAVATDFETMVKEGDKLAAIAPNVVVKLPLTWDGLRAARTFTDKGI
                     KTNVTLCFSAAQAMLAAKAGATFVSPFVGRLEDNGADGIGLLEEIRTLYDIHGFETEI
                     LAASLRNTNHVSAAAVAGSDAATLPADVFKALVKHPLTDKGLDQFLADWGKTGQSIL"
     gene            3451..>3951
                     /locus_tag="VF633_04930"
     CDS             3451..>3951
                     /locus_tag="VF633_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX8470434.1"
                     /translation="MTGTRDLFAKQETPMEPPHPHQVWPEVRVWLQANPQILLDDRSL
                     LEEIGLKPHGRNVVEFGRAALTRLEAVATRESDARKQIETVARANFAAQTQTHVAALD
                     LLEARNPSDLARRLDAAAQGRFGLAGAAIALEKPGGVPFGWRALEPGGVDNLLGEHGL
                     TWLGPNF"
BASE COUNT          677 a         1391 c         1275 g          608 t
ORIGIN      
        1 gcgcgtggtc aggatggtgc ccagaccatc atgatccagc gacccgccct cgaggatggc
       61 gacgtgacgc gtcaggggaa cgcctgctgc ctcaccgatc tggtcggcga cctcggtgtc
      121 gtgttgatag acgtatttgc cgccccagcc gttgaagccg aaggccgcgc cggtcttcga
      181 gttgacgccg aagatcggtc cggtgtcacg gagccaaatg tctccgaacc ggccggaaac
      241 gaccgtcacg ccctcgacgt ctgcaaaccg ggcctgggca tccgcgagcg cttcgtcatt
      301 gccgatcatc agcttgacct gctcgcggcc ggggcctgcg agggcgcgga caaggccttc
      361 gacctccgac tgcgcctgtt cgaagtcctc gccccacagt tcggcgtggc tcggccaacc
      421 gacccacatg gcccggtgag gagcccattc ggcgggaatg acctgtgaca tgggcggcga
      481 aaatcctgac gacgaacggg agggcttggg agcgcgtcca gatagacatt cgcccccctc
      541 gtttcaatga cagtcacccg tcgggcaccg tcagaggaag ctgtacgggt ccacgtcgat
      601 ggtcagccgc accgagccgg ggatcttcac ccgcgccagc cacgcccgca ggaacccctg
      661 cagatcgacg tcgcgatcgg cccgcaccag cagccgcttc ctccgccgac cccggatcag
      721 ggccagaggc gcgtcggccg ggccatagac ctccagccgc tcggcattcg gaatcgcctg
      781 agccaaagca tcggcggtgt tctccacggc gaccgcattc tcgcccgaca ggatcagggc
      841 cgcgagccgt ccatagggag gcagcgacgc cgcctcgcgt tcggccatct cggcctccac
      901 gaagccgtca cgatcgccgg cggccaacgc ccgcagcacc ggatgttcgg gcgtccacgt
      961 ttggaggagg gcgcgcccgg ggatgtcggc ccgccctgcc cggcccgtgg cctgcgtcag
     1021 aagctggaac gtccgctccg ccgcgcgcag atcgccgccc cgcaagccga ggtctgcgtc
     1081 gaccacgccg accagtgtca gcttgggaaa attgtgaccc ttggccgcag cttgcgtggc
     1141 gacgaggatg tcgatgtcgc cgtccaccat cgactggatc atggcccgcg ccgccttggc
     1201 gtccggcacg gtgtcggagc tgaaaaccgc cgtcctcgcc tgcgggaaca gggccttcac
     1261 ctcttcctcg acgcgttcga cgcccggtcc gacagctaca agtgaatccg acgcgccgca
     1321 ggacgggcag accttgggcc ggaccatgga gaagccggtc aggtgacaga ccagacggcc
     1381 ggtgtagcga tgctcgacca gccagctgtc ggtgtcgggc gcggtcagcc gatggccaca
     1441 agctcggcag aggacgacgg gggcatagcc gcgacggttc aagaacagca gcgtctgctc
     1501 tcgacgcagc aaggtctcgc cgatggcctc gcgcaacggc tgggacaacc atgtctgggg
     1561 atcgggcggg ttctgacgca ggtccagcag acggatatcc ggcatcaccg ccgccccgtg
     1621 ccgcgcacca agcttgagcc agcgatagcg gccctgatgc gcgttcagca gcgtctccag
     1681 cgacggcgtg gcagacgcca gcaccaccgc cgcgccttcg atccgggccc gcgccacggc
     1741 gaggtcgcga ccgtgataga cgaggccttc ttcctgcttg aacgaaccgt cgtgctcttc
     1801 gtcgaccacg atcagcttca ggttcgggaa cggcaggaac agcgccgagc gggccccgac
     1861 gacgatgttg cagcggcccg cgaccaccgc ctcccacacc tgacgccgac gtgggggcgc
     1921 gatacccgaa tgccactcag cgggagccgc gccgaaccgg tcggtcaacc gcgcgatcag
     1981 cgcctgcgtc agcgcgatct cgggcagcag gatcaggatc tgggccgtcg gatcggcccg
     2041 cagcgcccgc gccgccgctt ccagataggc ctcggtcttg cccgaccccg tgaccccgtc
     2101 cagcaggaag ggcgcgaaac cgcccttgtc gatggcctcg gcgatggcgg tggcagatgc
     2161 cgcctgatcc gcgttcaggg acgccggcgc gtggtcggga tcgggcgcat cgaaggcggc
     2221 gatggcctcg atctcgacga tctcaagcac gccctcatcc actagccctt tgacgacacc
     2281 agaggacacg cccgccgctc tcgccagatc gggacccggg atagagcgca cgcccaacgc
     2341 ctccagcaca gccgttcgcg atgcagtcgg gcgggcaggc tcccgcccct cgacacggca
     2401 cacgcgacgc tcgggcctag gacgcggcgc gcgcaaacct ttcagcgcgg tcgcggccat
     2461 ctcgccgggc gcgctgagcg tccagcgcgc cgcccattcg atgaaatcga ccgtgccggg
     2521 cggcaggcgc gggtcgtcca gcaccgtctc caccgccttg agccgacggt tcgagccggt
     2581 ggtctcgcgt acctcggaca cgatgccgcg catcagccgg ggccccaaag gcaccgcgac
     2641 ctgatcgccg cgttccaccg ccatatcgtg cgcgacctcg tagtcgaagg cttcgggcac
     2701 cggcagcgga atgaggactg aggcgacttt caagagccgc acgcctcgtt tacaaacatc
     2761 ggcatgaaac tcttcctcga caccgccgac gtcgccatca tcaaggacat gctgcccacc
     2821 gggatggtgg acggcgtgac caccaatccg tcgttgattg caaagtcggg gaggaacatc
     2881 gccgaggtca tcgccgagat ttgcgccctc gtcgaggggc cgatctccgc cgaggccgtg
     2941 gcgaccgact tcgagaccat ggtcaaggag ggcgacaagc tcgccgccat cgctccgaac
     3001 gtcgtcgtga agctccccct gacgtgggac ggcctgcgcg cggcgcggac tttcaccgac
     3061 aagggcatca agaccaacgt caccctttgc ttctccgccg cccaggccat gctggccgcc
     3121 aaggccggcg cgaccttcgt ttcgcccttc gtcggacggc tcgaagacaa cggcgccgac
     3181 ggtatcggac tgctcgagga gattcgcacg ctttatgaca tccatggttt cgagaccgag
     3241 attctggctg caagcctgcg caacacgaac catgtatccg ccgccgctgt ggcgggatcg
     3301 gacgcagcga ccctgcctgc tgacgttttc aaggccttgg tcaagcaccc gttaaccgac
     3361 aagggtcttg atcagtttct ggcggactgg ggcaagaccg gtcagtcgat tctgtagcat
     3421 acggcccggc ggttccggga ggagacgtcc gtgaccggca cccgagacct attcgccaag
     3481 caggaaacgc ccatggagcc accccaccct catcaggtgt ggcccgaggt tcgtgtttgg
     3541 cttcaggcca atccccagat cctgctggat gatcgctccc tcctggaaga gatcgggctg
     3601 aagccgcacg gacgcaacgt cgtcgagttc ggtcgcgccg ccctgacgcg tcttgaggcc
     3661 gtggccaccc gcgagtccga tgcccgcaaa cagatcgaaa cggtcgcccg ggccaatttc
     3721 gccgcccaga cccagaccca cgtcgcggcg ctggatctgc ttgaggcccg caatccttcc
     3781 gacctcgccc gacggctgga tgcagcggct caaggccggt ttggtctggc cggcgccgcg
     3841 attgcccttg agaagcccgg cggtgtgccg ttcggctggc gcgctctgga gcccggcggc
     3901 gtggacaatc tgctcggcga gcatggcctg acgtggctgg gtccgaattt c
//
LOCUS       DASVTM010000177         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_764507,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000177 DASVTM010000000
VERSION     DASVTM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764507"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VF633_04935"
     CDS             <1..544
                     /locus_tag="VF633_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470435.1"
                     /translation="KAHLLALPYWDPTRADGRSSAAMFSMLQRVHALKAAGRDVTLWA
                     YQPSTARPRGFDQSYTEIEMAGLLMDAAYARPDAIVLALGGSFHARKTIAPQYGFLFA
                     AGHLKAGNIVSLRTAFQGGRTWACFGEAPCGDTDLGQGDFGHERRGVILEPQNDGAYD
                     GLLALGPTTASPPARAAVAP"
     gene            631..1479
                     /locus_tag="VF633_04940"
     CDS             631..1479
                     /locus_tag="VF633_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8470436.1"
                     /translation="MLNQPHATVDGLSIPLLGFGTWMLEPDDARRMVAEALRIGYRHI
                     DTAWIYKNEAAVGDGIRDGIEAGHVTRDDIWLTTKIWVDHFEHDALLAQAKESATSLG
                     FTPDLLLLHWPKREPSFAETLGALNEAKDQGLTRSIGLSNFPSAEFREAQSLSKARLI
                     TNQVEYHPYLELTPLLATAAELGSSITAWSPLAQGKIADDAVINDIGRAHGKSAGQVT
                     LRWLIQQNVIAIPRTAKVSRAEENFDILDFELSDDEMKRIHALAQPDGRLGDWLDGAF
                     KWDKTG"
     gene            1502..1750
                     /locus_tag="VF633_04945"
     CDS             1502..1750
                     /locus_tag="VF633_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8470437.1"
                     /translation="MGAVLEFPEANPRHVSAGRRLKRWREQRGFDVDSLAAAIHATPD
                     VVRRVEAGRARLDSAQLAAATHALHLPLWALVSDTPAY"
     gene            2430..>2690
                     /locus_tag="VF633_04950"
     CDS             2430..>2690
                     /locus_tag="VF633_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX8470438.1"
                     /translation="MAYKSLRDFMAMLEADGELVRVSEPVSTHLEMTEIQTRLLRNGG
                     PAVLFEKPVMPDGSISPIPALANLFGTVKRVAMGVTMERKTRT"
BASE COUNT          500 a          880 c          818 g          492 t
ORIGIN      
        1 gaaggctcat ctgctggccc tgccatactg ggatcccacc cgtgccgacg ggcgctccag
       61 cgccgccatg ttctctatgc tgcagcgggt gcacgccctg aaggcagcag ggcgagacgt
      121 gaccctgtgg gcctaccaac caagcacggc gcgccctcgc gggttcgacc agtcctacac
      181 agaaatcgag atggcggggt tgctcatgga tgccgcctat gcccgccccg atgctattgt
      241 tctggcgttg gggggttcat ttcacgcgcg aaagaccata gcgccgcagt acggcttcct
      301 gttcgccgcc ggtcatttaa aggccggcaa catcgtctcc ttgcgaaccg cgttccaagg
      361 cggtcgcacc tgggcctgtt tcggcgaagc gccctgcggc gacaccgacc tcggccaagg
      421 cgatttcggc catgagcgca gaggcgtaat cctcgaaccg cagaacgatg gggcctatga
      481 cggtctgctg gcgcttggtc cgacgacagc ttctccaccc gcgcgggccg ctgtcgcacc
      541 ttgacgtccg ctagtgatca ggttgatcgg tggctctacc gacccgcgac ttcggcgcct
      601 aagtaaagcg acccaatcag gagcgcccaa atgctgaacc aaccccacgc caccgtcgac
      661 ggcctgtcca ttcccctgct cggtttcggc acctggatgc tggagcccga cgatgcgcgg
      721 cggatggtcg ccgaggcgct gaggatcggt tatcgccaca tcgacacagc atggatctac
      781 aagaacgagg ccgccgtcgg cgacggcatt cgtgacggca tcgaggccgg ccacgtcacg
      841 cgcgacgata tctggctgac gaccaagatc tgggtcgatc atttcgagca tgacgcgcta
      901 cttgcgcagg cgaaggagtc ggcgaccagc cttgggttca cccccgacct gttgttgctg
      961 cactggccca agcgggagcc gagcttcgcc gagacgctcg gcgcgctgaa cgaggcgaag
     1021 gatcagggcc tgacccggtc aattggcctg tcgaacttcc cgtccgccga gttccgagag
     1081 gcgcagagcc tttcgaaggc caggctgatc accaatcagg tcgaatacca cccgtacctc
     1141 gagctgacgc cgctgctggc gaccgcagcc gaactcggct cctcgatcac cgcctggtcg
     1201 ccgctggctc agggcaagat cgccgacgac gcggtcatca acgacatcgg ccgcgcccat
     1261 ggcaagtcgg ccggtcaggt gaccctgcgc tggctgatcc aacaaaacgt catcgccatt
     1321 cctcgcaccg cgaaggtctc gcgcgccgaa gaaaactttg acattctcga cttcgaactg
     1381 tcggacgacg agatgaagcg cattcacgcc ctggcccaac ccgatggtcg tctcggcgac
     1441 tggctggacg gagcgttcaa atgggacaag actggctgat cgcatcgaca ggaggccgct
     1501 catgggcgcc gtgctggagt ttcccgaggc caatccccgc catgtctcgg cggggcgacg
     1561 actgaaacgc tggcgcgagc agcgaggctt cgacgtcgac tcgctggcgg ccgccatcca
     1621 tgcgacgcct gatgtggtgc ggcgggtcga ggcgggccgc gcccgtctcg acagcgccca
     1681 gctggccgct gcgacccacg ccctgcacct gccgttgtgg gctctggtgt cggacacacc
     1741 cgcctattga tgacaaacgc ggcgacggtc tgcaggctcg tcttggcagc ggcaggctgg
     1801 ttcgccctat gggttccttg acatcggcgg gtaggctcag cggcctccgt gagctacgtc
     1861 ggccctacct tgttctttca gatcgtaggc acggcgatcg ttggcctgga ctggctcagc
     1921 cggctgcgcg agcacagtgg agcgtgagac cctgcccgcc tcttcctgcc gctgcatcat
     1981 ccggatcagg cgtcagaaac gtccttacga ggatgaacac tccagcccag aacgcgatcg
     2041 aaaagacgat agcagcgcta tatccaagca cgatccagtt gcggcgtcct tcgcccgaca
     2101 tccgttagcc tttccagcgt agtcgatcgg ttcaacgccg tcattccacc attttttcct
     2161 tgatttacaa tacctaaaaa gtgaattcgg gaggagctaa attcagactg cggtgcaaca
     2221 tgacgccgaa aatctgcgtt tccgtattaa aatcatcggg acgttgaccc ttgcccgccc
     2281 tctccggacg aggaataatt cgttgctcag cttcgctctt cgcaggagcg aagcagaatg
     2341 ccggttctta ccgagcgggg gcctgacagc atcgcgattt gacccttcgg gaaactgttc
     2401 taagccgtcg tctctgaccg gagtattcga tggcctacaa atccctgcgc gacttcatgg
     2461 ccatgctgga ggccgacggc gagctggtgc gggtgtccga gcctgtctcc acccacctcg
     2521 aaatgaccga gatccagacg cggctgctgc gcaacggcgg cccggcggtg ctgttcgaga
     2581 agccggtgat gccagacggc tcgatcagcc cgataccggc gctggccaac ctgttcggca
     2641 cggtcaaacg cgtcgccatg ggcgtgacca tggagaggaa gacccgcacc
//
LOCUS       DASVTM010000178         3670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_603611,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000178 DASVTM010000000
VERSION     DASVTM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603611"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2312
                     /locus_tag="VF633_04955"
     CDS             <1..2312
                     /locus_tag="VF633_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX8470439.1"
                     /translation="PDASELADYRPPTATRVYAGDGTLIGEFSDERRIYVPYEQIPQP
                     LIHAFLAAEDKNFFQHGGIDVSGLGRAMFKNVFNAVSGRRLEGGSTITQQVAKNVLLT
                     NESTVGRKLKEAILSSRLEATLTKEQILELYLNEIFLGYRSYGVASAAYNYFGKSLQQ
                     LSPDEAAFLASLPKGPNNYHPKRHPGAAKGRRDWVLGEMEQNHWITEAQMIEGRARPL
                     ITQDAPKRAQYADADFFVEEARRQAAANPDIGGQLNAGGFYMRTTLDPTLQTAARDAL
                     MTGLENYDRRHGWRGAWSTTDFAEGWQALALKKNVPPERRAWQAAAITAVSGNSVQIV
                     TARDGETGSLIVADAAWANANKQLRRGDLIFIEKTGGQFALKQIPIVNGALVAIEPQS
                     GRVLAMVGGYSYALSSFNRATQAERQPGSSFKPFVYATALESGDFTPASIVMDAPISF
                     AGGANGGRWTPENYSRQYYGPSTLRRGLELSRNVMTVRLASVIGMPRIIDLAKKMGVS
                     DNLQPNLSVSLGAGETTPYRLTAAYAAFVNGGRRVDPYLIEMVQDRNGETVYRADSRQ
                     CRECDRSFGGQPSPRLEPRGTQVIDPITAYQISSMLEGVVQRGTAASARGLGRWIGGK
                     TGTTNDYRSAWFVGFTTDIVVGVFIGFDDNRSLGSGETGAGTPVPIFVDFMHTALRER
                     PARPFVRPKNATFRMVNGIEEAFRPGTERERRDEAPRPNVPTGPMNYNEVIRQEQMGV
                     SPQPGSATPPPSAPPPPQKAPAEDLSGLY"
     gene            2508..3630
                     /gene="prfB"
                     /locus_tag="VF633_04960"
     CDS             join(2508..2576,2578..3630)
                     /gene="prfB"
                     /locus_tag="VF633_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269601.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX8470440.1"
                     /translation="MRPDVEAMKADIEQSVALLRRRLDWDVSLRKLDELNARVEDPTL
                     WNNPEAAQAVSRDRARLEGQINAVRAMERELEDGVMLSEMAEEENDEATLDEARVQLR
                     GIKERAARAELEALLSGEADGNDAYLEVNSGAGGTESNDWAGMLLRMYSRWAKAHGYE
                     VQIEAEESGEQTGVKSATILVTGPNAYGWLKSESGVHRLVRISPYDAAAKRHTSFASI
                     GVSPVVDDAIVIDINPSDVRTDTYRASGSGGQHINKTDSAVRLTHIPTNTVVACQAGR
                     SQHQNREQAWKMLRARLYELELQTREAAAQAMEDAKTDIGWGHQIRSYVLQPYQMVKD
                     LRTEVETSDTQGVLDGDLDLFMGAALAQRVGETRGSSTE"
BASE COUNT          676 a         1253 c         1134 g          607 t
ORIGIN      
        1 tgccggatgc gtccgagctg gctgattatc gccccccgac cgcgacgcgg gtctatgcgg
       61 gcgacggcac cctgatcggt gagttctcgg acgaacgccg gatctacgtg ccatacgagc
      121 agattccgca gccgctgatt cacgcctttc tcgccgccga agacaagaat tttttccagc
      181 acggcggcat cgacgtctcc ggtcttggcc gcgccatgtt caagaacgtg ttcaacgcgg
      241 tttctggtcg acgtcttgag ggtggctcga ccatcaccca gcaggtcgcc aagaacgtgt
      301 tgctgaccaa cgaatcgacg gtcgggcgca agctgaagga ggcgatcctc tccagccgcc
      361 tcgaagcaac gctgaccaag gaacagatcc tcgaactgta tctgaacgag attttcctcg
      421 gctaccggtc ctatggcgtg gcctcggccg cctacaacta tttcggcaag tcactgcagc
      481 agctttcgcc tgatgaggcc gctttcctgg cgtcgctgcc caagggaccg aacaactatc
      541 atcccaaacg ccacccgggc gcggccaagg gccggcgcga ctgggtgttg ggcgagatgg
      601 agcagaacca ctggatcacc gaggctcaga tgatcgaggg ccgggcccga cctctgatca
      661 ctcaggatgc ccccaagcgt gcgcaatacg ccgacgccga cttcttcgtg gaggaagccc
      721 gtcgtcaggc ggctgcgaac ccggacatcg gcggccagtt gaacgccggc ggcttctata
      781 tgcgcactac gctggatccg acgctgcaga cggcggcgcg tgacgccttg atgaccggac
      841 tcgagaacta tgaccgtcgc cacggctggc gcggagcatg gagcacgacg gacttcgccg
      901 agggctggca ggcgctggcg ctcaagaaga acgtgccgcc agaacgccgc gcctggcagg
      961 cggcggcgat caccgcagtc tccggcaaca gcgtccagat cgtcaccgcg cgtgacggcg
     1021 agacgggcag cctgatcgtc gccgacgccg cctgggccaa cgccaacaaa caattgcgcc
     1081 gtggtgacct gatcttcatc gagaagacgg gcggccagtt cgccctgaag caaatcccca
     1141 tcgtcaacgg cgccctcgtc gcgatcgagc ctcagtccgg acgggtgctg gcgatggtcg
     1201 gcggctattc ctacgccctg tccagcttca accgcgccac acaggccgag cgtcagcccg
     1261 ggtcgtcgtt taagccgttc gtctatgcga cggctctgga aagcggagac ttcaccccgg
     1321 cctcgatcgt catggacgct ccgatcagtt tcgcaggcgg cgccaacggc ggtcgttgga
     1381 cgcctgagaa ctacagccgc cagtattatg gcccctcgac cctgcgtcgt ggcctcgaac
     1441 tgtcgcgtaa cgtcatgacc gtgcgactgg cctcggtcat cggcatgccg cgcatcatcg
     1501 acctcgccaa gaaaatgggc gtgtctgaca accttcagcc caacctgtcg gtgtcgctgg
     1561 gggcagggga gaccaccccc taccgcctga ctgctgccta tgccgcgttc gtcaacggcg
     1621 gccgccgggt ggacccttat cttatcgaga tggtgcagga ccggaacggc gagacggtct
     1681 atcgcgccga ttcgcgccag tgccgcgagt gcgatcgcag cttcggcggc cagccgtcgc
     1741 cgcgcctcga accgcgcggg acccaggtca tcgacccgat taccgcctat cagatcagct
     1801 ccatgctgga aggcgttgtc cagcgcggca ccgccgccag cgcccggggc ctgggccgct
     1861 ggatcggcgg caagaccggc acgaccaacg actatcgctc ggcctggttc gtgggcttca
     1921 ccaccgacat cgtcgttggc gtcttcatcg gcttcgacga caaccgctcg ctgggatcag
     1981 gcgagaccgg tgcgggaacg cccgtgccga tcttcgtcga cttcatgcac accgccctgc
     2041 gtgagcgtcc ggcccgtccg ttcgttcgac ccaagaatgc gaccttccgg atggtcaacg
     2101 gcatcgagga ggccttccgc cccggcaccg agcgcgagcg ccgtgacgag gctccgcgtc
     2161 ccaatgtccc gaccggcccg atgaactaca acgaggtcat ccgtcaggag cagatgggcg
     2221 tctcgccaca acccggttcc gcaaccccgc caccttccgc cccgccaccg ccgcagaagg
     2281 ctccggctga ggatctgagc ggattgtact aagggaggca gtaggcaggt aggcaacagg
     2341 cagtaggagt gaagcggggg gccaccgtgc cgttctggct accgtccttg tttctaccgc
     2401 ctactgccta cctgcctact gcctacctgc ctattgcctc ctaacccctt cctctttccc
     2461 ccagcgcgct ctatgtccgc gctcctctta cactcaggat ttttgccatg agaccggatg
     2521 tcgaggccat gaaggccgac atcgagcaga gcgtcgctct gctcaggagg cgtctttgac
     2581 tgggatgtct cacttagaaa gctcgatgag ctgaacgcgc gggtcgagga cccgacgctg
     2641 tggaacaacc cggaagcggc ccaggccgtg agccgcgata gggcccgtct ggagggccag
     2701 atcaacgctg tccgcgccat ggagcgcgag ctcgaggacg gcgtcatgct gtccgaaatg
     2761 gccgaagaag aaaacgacga ggccaccctc gacgaggccc gcgtccagct tcgcggcatc
     2821 aaggagcgcg ccgcccgcgc cgagctcgag gccctgctgt ccggcgaggc cgacggcaac
     2881 gacgcctatc tggaagtgaa ctccggcgcc ggcggcaccg agtccaacga ctgggccggc
     2941 atgttgctgc ggatgtactc gcgctgggcc aaggcgcatg gctatgaagt tcagatcgag
     3001 gccgaggagt ccggcgaaca gacgggcgtc aagtcggcga ccatcctcgt caccggtccc
     3061 aacgcctatg gctggctgaa gtccgaatca ggcgtgcacc gtctggtccg catcagcccc
     3121 tatgacgccg ccgccaagcg ccacacctcg ttcgcctcga tcggcgtttc gccggtggtg
     3181 gacgacgcca tcgtcatcga catcaaccct tcggacgtcc gcaccgacac ctatcgcgcc
     3241 tcgggctcag gcggtcagca cataaacaag acggattcgg ccgtgcgtct gacgcacatc
     3301 ccgaccaaca ccgtcgtcgc ctgtcaggcc ggtcgctccc agcaccagaa ccgcgaacag
     3361 gcctggaaga tgctgcgggc ccgtctttac gaactggaac tgcagacccg cgaagccgcg
     3421 gcccaggcca tggaagacgc caagaccgat atcggctggg gtcaccagat ccgctcctac
     3481 gtcctgcagc cctaccagat ggtcaaggac ctccgcaccg aggtcgagac ctccgacacc
     3541 caaggcgtcc tcgacggcga cctcgacctg ttcatgggcg ccgcgttggc ccagcgggtc
     3601 ggcgagaccc gagggtcgag caccgaataa gattagggcc cggagcgatc cgggccctgt
     3661 ttggtcagtg
//
LOCUS       DASVTM010000179         6882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_817542,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000179 DASVTM010000000
VERSION     DASVTM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817542"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VF633_04965"
     CDS             <1..1548
                     /locus_tag="VF633_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470441.1"
                     /translation="LAADLAAGLEAIHARGQLHLNLAPESIGVASGRGVLHDPSADRR
                     RYIPLVRRLDGLFRTGLSPFEAQDATESAPLTPATDIYGLCAVLYRVVTGRDAPDWFG
                     WSDDTRLSAQPEAAGYPAAFVAAVEKGLSRNAADRVASVSDWRAAAGVGEPAPVEGRV
                     WFDAVEDFRVPPAPPGPVDELALAPPSFAGPADPVVVDDIGALRRSPEPVPFTPSRTV
                     TPSPQPRRSKAFGIGAVAAVAGLVAVVAFAGSRLTGDSSKASADEGKVSTRAAPPRAP
                     DGCAYRTGEDADDLTLRCGPGEGQVVDRGALRVSDVADAVAGRPGAMARLGAFYLRDT
                     PGRDERAGLDWLGRAAAAGETSAMLDLADFYETSATDASAAGTPAALRQSLQWYRTVS
                     DPGQGAHMPSPDQIRLADEGVQRIEARLTEVRDAAFVGCWRSGDVVADLRIDNDIFTL
                     AVEGGEPAVGQIAEPRQDDRIAVRLDSPGLMSGQRYLFSRVGADMIEHRLDVSTENAR
                     WNRCSAE"
     gene            2058..2741
                     /locus_tag="VF633_04970"
     CDS             2058..2741
                     /locus_tag="VF633_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8470442.1"
                     /translation="MAAPTAIANAPQGLDLSLGQEHLRAAQAGSVSAMGKVAEFYRSG
                     DGGLGQHPDAAVEWARRADAHGDPWGRYILGLCFEAGDGELAEDPDEAYRLFRLAADA
                     GVTPAMGKVGEYLYQGWGAVPRDDDEALIWLDRAADAGDARANYYLALFHLDGLAGLE
                     PDPDTAAALMLTSAQAGDVRAMFRTGRNYEIGMGIGRDLFEARRWYRQAADAGDPDAR
                     ERLDVIGGY"
     gene            2832..3362
                     /locus_tag="VF633_04975"
     CDS             2832..3362
                     /locus_tag="VF633_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470443.1"
                     /translation="MATAEDDGLDQIRRDLIELVLREAGGRVAADPKSPVAQLLAGIV
                     RREMAGAVEELRHGGAVVDTEALAEAIARRMGSAPRGQDFRSTSTASGANRTREIIVG
                     CVAAVVAVAIFVGGFALGGGLTKVQPAPIAAQTEAPVDPALVAPATSDGTVPTATPPV
                     SGDGATGMANQLSERP"
     gene            3362..4924
                     /locus_tag="VF633_04980"
     CDS             3362..4924
                     /locus_tag="VF633_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470444.1"
                     /translation="MSLTSLETAVLGPVENGRGYTVLARSTAAPGQDAAVEGMLRHFS
                     LALAGWADRQPSDCVVMFPLDAAAGTFAVARVAFLGEAQLGTVAMAHVLVLPLTAMTA
                     LDWLSHRLLAHIPTPTAEDDLSFADASLKLDLEVLAQGEAGPRLASFGLEWRDHEIAV
                     GAANPEAVLAAAIEGMDPPEQRARLTGWVTSGAFTRSGAFDPDEAFRLIVRGDGEGEA
                     QPNTGRRLVRLTNGQIAPVAATDATLAPPVAWRVWAQVEATIRARGVGKGLEWQPRYA
                     DHAPDAIAAIAILEACLDLDPVGRAALLSAVAESSDETDDGPDLLKGAAIALGRLMDQ
                     PGEPEGAAWYLNDYIEASADRPAAVASVAHLALRDGVLPWMSAQSLDLLAFAGLADRL
                     AAQAGYPLDGLPVAARLRLLKASIQKPGMVERRALTVRLIGLCLPDSAGAKPCAQAIA
                     ALLALPAGPEDAALATPAIYDLLQVGGSSVRTAYARRVLSPVLREQVRLAREPYVMAL
                     RTALHAVSGDAR"
     gene            4924..6489
                     /locus_tag="VF633_04985"
     CDS             4924..6489
                     /locus_tag="VF633_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470445.1"
                     /translation="MATKTCPIHFIDYPEQLDECPYCVEVRREEAAKPRPPETPTPGE
                     DQSSLLAGIDTLAGSGSIRTPIASFAPDAVITPDFTTQTRENPVSPSAPDTTGATDLT
                     AVTQPAQVEPVTDAAEAVLTLAKPVLRRPERGLAGTPEHDQPVSETLLNSWKNADQNI
                     VVLLGFASAGKSWFLTRLKHKLFHDPSFPLRSEPAFADQGARVSRSNNISSHSFLPVS
                     GAEGAHADDVFHIIDMPGERFEGVAGIDLFGRREELLALQVCKSVIIVLPADEVLLAE
                     RAAERYALWEDETAPAKARVPKAQQTLETQARDLAKANPQLERFIKTLGRLQDLLSLL
                     ETGIDVDAIIAMGRDGVIAHAATAKRPSKPVFIGLAKADVIKDYIREIQDAYADGVPD
                     KPVNLLHLLFKDAEEDLLALERFDDDPLTTVRRFRRGLVQCMEGFRWCKLDFVTAFAE
                     SEDPARRKRDPLRVDYGRRSWGVVPVIRWIQWAGRGRQISSQSDWAFISAARAIRRFR
                     DDGRLTPLKRRGL"
     gene            6486..>6882
                     /locus_tag="VF633_04990"
     CDS             6486..>6882
                     /locus_tag="VF633_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470446.1"
                     /translation="MSDATPLKPPFAQRLRRWRMDGIDLVFGEADRRPVQVLSAVCVA
                     IWLIVALWLSGFPSPWGGERGAIVIPAGAESDDGLGAAAANSAFQWAEFLRSDGEAPD
                     GLPPFPSGRGQERAWLDRHVCGPFSKAQSN"
BASE COUNT         1039 a         2357 c         2348 g         1138 t
ORIGIN      
        1 ctcgccgccg atctggccgc cgggctcgag gcgatccatg cgcggggaca actgcatctg
       61 aacctggccc ccgagagcat cggcgtggcg agcgggcggg gtgttctgca tgatccgtcg
      121 gccgatcggc ggaggtacat cccgctggtg cggcggctcg acggtctttt ccgcacaggg
      181 ctgtcgccgt tcgaggccca ggatgcaacc gagagcgcgc ccctgacgcc tgcaacggat
      241 atctatggcc tgtgtgcggt gctgtatcgc gtggtgacgg ggcgggatgc ccccgactgg
      301 ttcggctgga gcgacgacac gcggctgtcc gcacagcctg aggcggcggg atatcctgcc
      361 gccttcgtcg ccgccgtcga aaaagggctg tcgcgcaacg cagccgaccg ggtcgccagc
      421 gtctccgact ggcgcgccgc tgccggtgtg ggcgagcctg cgcccgttga gggccgcgtc
      481 tggttcgatg cggtcgagga tttccgcgtc ccgcccgcgc cgccgggccc ggtcgacgaa
      541 ctggccctgg ctccaccttc gttcgcgggc ccggcggatc cggtcgtggt ggacgacatc
      601 ggggccttgc gtcgctcgcc ggaacccgtg ccgttcaccc cgtcgaggac cgtcacgccc
      661 tcgccccagc cacgtcgcag caaggcgttc ggcatcggcg ccgtcgcggc cgttgcgggt
      721 ctcgtcgcgg tggtcgcgtt cgccggctcg cggctgaccg gcgacagctc gaaggcctct
      781 gcggacgaag gaaaggtatc gacccgggcc gcgccgcctc gcgcgccgga cggctgcgcc
      841 tatcggacag gcgaggacgc cgacgacctg accttgcggt gtggtccggg cgagggacag
      901 gtggtcgatc gtggcgcact cagggtctcg gatgtcgccg acgccgtcgc cgggcggccc
      961 ggagcgatgg cccggctggg cgccttctat ctgcgcgata caccgggccg ggatgagcgc
     1021 gcggggctcg actggctcgg gcgcgcggcc gcggcaggcg agacatccgc catgctggac
     1081 ctcgccgact tctacgagac atccgcgacc gatgcgtccg ccgcaggaac gccggccgcc
     1141 ctgcgccaga gcctgcagtg gtatcggacg gtcagcgatc ccgggcaggg tgcgcatatg
     1201 ccgtctcccg accagatccg gctcgcggac gaaggcgtgc agcgcatcga agcgcggttg
     1261 accgaggtgc gcgacgctgc cttcgtgggg tgctggcgct caggcgacgt ggtcgccgat
     1321 ctgcgcatcg acaacgacat cttcaccctc gcggtcgagg gcggcgaacc tgccgtcggt
     1381 cagatcgccg agccgcgtca ggatgatcgc atcgcggtca ggctcgatag ccccgggttg
     1441 atgagcggcc agcgctatct gttcagccgc gtcggcgccg acatgatcga gcaccggctg
     1501 gacgtctcga ctgaaaacgc gaggtggaac agatgttccg ccgaatagcg cccgtcctga
     1561 tcctgttggc cgccgtatcc ggggcgccgg cccacgccca gattgcgggc ggtctgcgcc
     1621 tcgagggtgt ggggacctgc gcccaactca gcagtttcat cgcccgcaat ccgggcttcg
     1681 acagcgccat gatcgcgggc aagcgggatg atctcggcgg ctgtgcgcca gcgccgacgc
     1741 ctgtcccaag gcctcgacca ggaccacaac caagaccaca accaagacca caaccaagac
     1801 cacagccgag acccgcgcct ccggtcaggc ctgctccgac cccacgcgtt ccgccccgca
     1861 tcccggctcc gaccgtgccg cccatcgcgc ttgctccggt cccgacgcct gcgccagagc
     1921 cccgtccgat accgccccgg ccggttggcc ccagcatcac gccgcgcctc aacgcgccca
     1981 gcctgacgcc gcgtctcaac ctgacaccgc acgttgtcac cgggagcgcg accaacaccc
     2041 ttaacgcagc gccagatatg gccgctccta ccgccatcgc gaacgcgccg cagggtctcg
     2101 atctatcctt ggggcaagag catctgcgtg cggcgcaggc gggcagcgtg tccgccatgg
     2161 gcaaggtcgc ggagttctat cgcagcggcg acggcggcct cggccaacat cctgacgccg
     2221 ccgtcgagtg ggcgaggcgg gccgatgctc acggggatcc gtggggccgc tacatcctcg
     2281 gcctgtgctt cgaggccggc gatggggagt tggccgagga ccccgacgag gcttatcggc
     2341 ttttccgtct cgccgccgac gccggggtga cgcctgcgat gggcaaggtc ggggagtatc
     2401 tctatcaggg atggggcgcc gttccgcgcg acgacgacga ggccctgatc tggctggacc
     2461 gcgccgctga cgcgggcgac gcccgggcga actactatct cgccctgttc catctggacg
     2521 gcctggcggg gctggaacct gatcccgaca cggccgccgc cttgatgctg acatcggctc
     2581 aggccggaga tgtgcgggcg atgttcagga cggggcgcaa ctatgagatc ggcatgggta
     2641 tcgggcgcga tctgttcgag gcccgtcgct ggtatcgtca ggctgcggac gccggtgatc
     2701 ccgacgctcg cgagaggctc gacgtcattg gtggatattg atcgcatgac agttttccgg
     2761 cccgaacctg tcacgtcgtt cacgtgcgtt cgggccggaa tcgtttaggt atggcgcagg
     2821 tggagcagca aatggcgaca gctgaagatg acggcctcga ccagatccga cgcgatctga
     2881 tcgagctggt cctgcgcgag gctggcgggc gcgtggctgc ggacccgaag tcgcccgtcg
     2941 cccagcttct ggccgggatc gtccgaagag agatggccgg agccgtcgag gaattgcgtc
     3001 atggcggcgc cgtggtcgac accgaagctc tggccgaagc gatcgctcgc cgaatgggct
     3061 cagctcctcg aggacaggat tttcgttcga catccacggc ttccggcgca aatcgaacac
     3121 gggagatcat cgtcggctgc gtggccgcgg tggtggcggt cgccatcttc gtcggcggtt
     3181 tcgccctcgg cggcggcctg acgaaagtgc agccggctcc gatcgcggcg cagaccgagg
     3241 cgccggtcga tcctgcgctc gttgcgcctg cgacctcgga tggaacagtc ccgacagcga
     3301 cgccgccagt gtccggcgac ggcgcgaccg gcatggccaa ccagttgagc gagcggccct
     3361 agtgagcctg acgtcgctgg agacggccgt tcttggcccg gtggagaatg gccggggcta
     3421 cacggtcctt gcccgctcga ccgccgctcc cggtcaggac gcagcggtcg aagggatgct
     3481 gcgccatttc agcctggccc tcgcgggatg ggccgatcgc cagccgtcgg actgtgtcgt
     3541 catgttcccg ctcgatgcgg cggccgggac cttcgcggtc gcccgggtcg cctttctcgg
     3601 agaagcccaa ctcggaacgg tggccatggc gcatgttctc gtcctgcccc tgaccgcaat
     3661 gacagcgctc gactggttat cccaccggct gctcgcccac atcccgacgc cgacggccga
     3721 ggatgatctg agcttcgcgg atgcatcgct gaaactcgat cttgaggtgt tggcgcaagg
     3781 ggaagccggt ccgcgtctgg cgtcgttcgg gctggagtgg cgcgatcacg agatcgccgt
     3841 gggagccgca aacccggagg ccgttctcgc cgcagccatc gaggggatgg atccgcccga
     3901 gcagcgcgcc cgtctgaccg gttgggtgac gtccggcgcc ttcacccgct ccggcgcctt
     3961 tgatcccgac gaagccttcc gtctgatcgt gcgcggagac ggggagggcg aggcgcagcc
     4021 gaacaccgga cgcaggctcg ttcgactgac caacggtcag atcgcgccgg tcgccgcgac
     4081 tgatgcgact ttggcgccgc ccgtcgcctg gcgcgtctgg gctcaggtcg aggcgaccat
     4141 ccgcgcccgc ggcgtcggca aggggctcga atggcagccg cgctacgccg accacgctcc
     4201 cgacgccatc gcagccatcg ccattctcga agcctgtctc gatctcgacc cggtaggccg
     4261 cgccgcgctt ttgagcgccg ttgccgagag ttccgacgaa acagacgacg gtcccgatct
     4321 gctcaaggga gcggccatcg ctctcggtcg gctgatggat caaccggggg agcccgaggg
     4381 tgccgcgtgg tatctgaacg attacatcga ggccagtgcc gatcgcccgg ctgccgtggc
     4441 atcggtcgct cacctcgcat tgcgtgacgg cgtcctgccg tggatgtcgg cgcagtcgct
     4501 ggatcttctg gcgttcgccg gactcgcgga tcgtctggcg gcgcaggccg gctatcctct
     4561 cgacggcctg ccggtggcgg cccgcctgcg cctgctgaaa gcctccattc agaagcccgg
     4621 catggtcgag cgtcgcgccc tgacggtgcg attgatcggc ctgtgcctgc cagattccgc
     4681 cggagcgaag ccttgcgcac aggcgatagc cgcccttctg gctctgcccg ccgggcctga
     4741 ggatgctgcc ctggccaccc ccgccatcta cgaccttctg caagttggag gttcgtccgt
     4801 ccgcaccgcc tatgcccggc gcgtgctctc gcctgtcctc agagaacagg tgaggttggc
     4861 cagggaaccc tatgttatgg cgttgagaac tgcgctgcac gctgtgagcg gagacgcgcg
     4921 ctgatggcca ccaaaacctg tcccatccac ttcatcgact atccagagca actcgatgaa
     4981 tgcccgtact gcgtcgaggt ccgtcgtgag gaggccgcca agccccgacc gccggaaacg
     5041 ccgacgccgg gtgaggatca gtcctcgttg cttgcaggaa tcgacaccct cgcggggtcc
     5101 ggatcgatcc ggaccccgat cgcatccttc gcaccggatg cggtgatcac tccggacttc
     5161 acgacgcaga cgcgcgaaaa ccctgtgtca ccatcagcgc cagacaccac aggcgcaacc
     5221 gacctgacgg ccgtcacgca gccagctcag gtcgagccgg ttaccgacgc cgccgaggcc
     5281 gttcttacgc tggccaagcc tgtgctgcgc cgtccggagc gcggcctcgc cgggacgccg
     5341 gaacatgatc agccggtcag cgaaaccctg ctgaacagct ggaagaatgc tgatcagaat
     5401 atcgtcgtgc tgctgggctt cgcctcggcg ggcaagtcgt ggttcctgac gcggctgaag
     5461 cacaagctgt tccacgatcc gtcgttcccg ctgcgctccg agcccgcttt cgccgatcag
     5521 ggtgcgcgcg ttagccgctc gaacaacatc tcctcgcaca gcttcctgcc tgtctcgggt
     5581 gctgaaggcg cccacgccga cgacgtgttc cacatcatcg acatgcccgg agaacggttc
     5641 gagggcgtgg ccggtatcga cctgttcggg cgccgcgagg agctgctggc gctgcaggtg
     5701 tgcaagtcgg tgatcatcgt cctgcccgca gatgaggtgt tgctggccga gcgcgccgct
     5761 gaacgctacg ccctgtggga ggatgagaca gctccggcga aggcccgggt acccaaggcc
     5821 cagcagacgc tggagaccca ggcgcgcgat ctggccaagg ccaatcccca gctggagagg
     5881 ttcatcaaga cgctgggccg tctgcaggac ctgctgtctc tgctggaaac cggcatcgac
     5941 gtcgacgcga tcatcgccat gggccgcgac ggcgtcattg cccatgccgc caccgccaaa
     6001 cggccctcca agccggtctt catcggtctg gccaaggccg acgtcatcaa ggactacatc
     6061 cgcgaaattc aggacgccta tgccgacggc gtgcccgaca agccggtcaa tctgctgcac
     6121 ctgctgttca aggacgccga ggaggatctg ctggcgctcg agcggttcga cgacgatccg
     6181 ctgaccaccg tgcgccggtt ccggcgcggt ctggttcagt gcatggaggg ctttcgctgg
     6241 tgcaagctcg acttcgtcac cgcctttgcc gagtccgagg atcccgcccg gcgcaagcgc
     6301 gatccattgc gggtcgacta tggccgccgc agttggggcg tggtgccggt gatccgctgg
     6361 atccaatggg cggggagggg acggcagata tcgagccagt ccgactgggc cttcatttca
     6421 gccgcccgcg ccattcgccg cttccgtgac gacggccgtc tgaccccgtt gaagaggcgc
     6481 ggcctgtgag cgatgcgacc ccgctgaaac cgcctttcgc ccaacgtctg cgtcgctggc
     6541 gcatggatgg gatcgacctc gtttttggcg aagcggaccg gcggccagtg caggttctct
     6601 ccgccgtctg cgtcgccatc tggctgatcg tggcgctctg gctctccggc tttccatcgc
     6661 catggggcgg agagcgcggc gcgatcgtga tcccggcggg ggccgaaagc gacgacgggc
     6721 ttggcgcagc ggcggcgaac tccgctttcc agtgggccga gttcctgcgc agcgacggcg
     6781 aggctccgga cgggcttccg ccgttcccga gcggcagggg gcaggagcgg gcctggctgg
     6841 atcgccacgt ctgcggtccg ttctccaaag ctcaatccaa cc
//
LOCUS       DASVTM010000180         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_284829,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000180 DASVTM010000000
VERSION     DASVTM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284829"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VF633_04995"
     CDS             complement(<1..453)
                     /locus_tag="VF633_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470447.1"
                     /translation="MPPFLALLVVHISAGFCAVGVGLMPILTSKGSRLHRLSGRVFAV
                     LMTILLICAWAMTALRPDAYFLALSATASLTLFSGLRVLGRKRPDLRTTDRARPLDWI
                     ATLAIVGIGILLMWLALTGRSDAPPAIVIALAYGSLSLGGWDLWRFARP"
     gene            complement(531..1286)
                     /locus_tag="VF633_05000"
     CDS             complement(531..1286)
                     /locus_tag="VF633_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8470448.1"
                     /translation="MTTTHPLADRIALVVGASRGIGFESALALAKAGAHVVATARTQG
                     GLEELDDHIFRETGKHATLVPFDLVDGGGIDRLGGAIFKRFGKLDIWVNAAATLGAAG
                     LTPVAHLDPRGWAKIEKTNFTSVFRLIRSLEPLLRLSDAGRVIHLTSSLATTPRAFWG
                     AYAATKAGAEAMMKAWADEIESTPVRVSIVDPGRMRTAMRGQAYPGEDPETVPHPSEI
                     APLIVELARGDFEPPLMVSFKDWSAGPSGEALI"
     gene            complement(1322..1672)
                     /locus_tag="VF633_05005"
     CDS             complement(1322..1672)
                     /locus_tag="VF633_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8470449.1"
                     /translation="MAQVQLGYFTLDTADIGKAKAFYGALFGWTFDEDASRSTYAHVS
                     NSDPAFGFVKAERPASEPNLYFKVENITAACARVTELGGKASIPAESATGRSCTVCDD
                     QGVSFSLWQPGEGY"
     gene            complement(1701..>3115)
                     /gene="purF"
                     /locus_tag="VF633_05010"
     CDS             complement(1701..>3115)
                     /gene="purF"
                     /locus_tag="VF633_05010"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX8470450.1"
                     /translation="VWGAAQDEASSIVALGLHALQHRGQEACGIASVNDTRFHTERHM
                     GHVGEAFGGTDLKTRMPGAAAVGHTRYSTAGGSFLRNIQPMFADLDQGGIAIGHNGNL
                     TNFHYLRNQLVGEGSIFQSTSDSEVILHLIARSRKAKIVDRFIDALARIEGGYALVAQ
                     TRTKMIGARDPLGIRPLVLGKIGEAWVLASETCALDTIGATFVRDVEHGEVVVIDKHG
                     LQSIKPFPARAARPCLFEYVYFSRPDSVVNGRSVYGVRKRMGQGLAREFPIEADVVVP
                     VPDSGVPAALGYAQESGIPYEMGIIRSHYLGRTFIQPSQGARQKGVAMKHSPNRSVIE
                     GKRVVLIDDSIVRGTTSVKLVRAVRDAGATEVHLRSASPPILWPDFYGIDMPERDQLI
                     AANKTMEEMRELLEVDSLGFLSVDGLYKAMGETGRNNRHPQFTDHYFTGEYPTRLVDR
                     EIEEGGRETVGRQLSLLVSA"
BASE COUNT          559 a          960 c         1081 g          515 t
ORIGIN      
        1 cggtcgtgca aaccgccaca ggtcccatcc ccctagcgag agtgagccat aggcgagggc
       61 gatgacgatc gcgggcgggg cgtcgctgcg gccggttagc gcaagccaca tgagcaagat
      121 gccgattccg acgatggcca gcgtggcgat ccagtcgagc gggcgggctc ggtcggtcgt
      181 tcgaagatcg ggtcgctttc ggcccagcac ccgcaagccc gagaaaaggg tcaaggacgc
      241 cgtcgcagag agggcaagga aataggcgtc gggtctgagc gccgtcatgg cccaggcgca
      301 gatcagcagg atggtcatca gtactgcgaa cacccggccg gacagccggt gcagccgtga
      361 acccttgctg gtcaggatgg gcatgagacc gacgccgacg gcgcagaagc cggcgctgat
      421 gtggacaacg aggagagcaa gaaacggggg catggccgac cctgtggctc gtcgtagtct
      481 cgtcgccgag catagcggga cgaacgacgg ctggcaggga cgagcgtcgc ctagatgaga
      541 gcctcgcctg aaggaccggc cgaccaatcc ttgaacgaca ccatcagcgg gggctcgaaa
      601 tcgccgcgcg cgagttcgac gatcagcggc gcaatctccg acggatgcgg caccgtctcg
      661 gggtcttcgc cgggataagc ctgtccgcgc atggccgtgc gcatacggcc agggtcgacg
      721 atggagaccc gcacgggggt ggattcgatc tcgtcagccc acgccttcat catcgcctcg
      781 gcgcccgcct tggttgcggc gtaggccccc cagaaagcgc gcggcgtggt cgccaagctg
      841 ctggtcaggt ggatcacccg cccggcgtca gacagacgca gcaggggctc caacgaacgg
      901 atgaggcgga acaccgaggt gaagttggtc ttctcgatct tggcccagcc gcgcgggtca
      961 agatgggcga cgggcgtcag gcccgcagcg cccagcgtcg ccgccgcatt gacccagata
     1021 tccagcttgc cgaaccgctt gaagatcgct ccgccgagcc ggtcgatgcc tccaccgtcc
     1081 accagatcga acggcaccag cgtcgcgtgc ttgcccgtct cgcggaagat atggtcgtcg
     1141 agttcctcga gcccgccctg cgtacgggcc gtggccacga catgggctcc ggctttggcc
     1201 aacgccagcg cgctctcgaa gccgatgcca cgcgacgctc ccacgacgag agcgatacga
     1261 tcagcgaggg gatgtgtcgt ggtcatcgcg cctgataacc cggcgttagc ctgcgggaaa
     1321 gtcagtaacc ttcgccgggc tgccacaggc taaagctgac gccttgatcg tcgcatacgg
     1381 tgcaggaccg accggtagcg ctttcggcgg gtatgctggc cttgccgccc agttctgtaa
     1441 cccgggcgca cgcagccgtg atgttctcca ccttgaaata gaggttcggc tcgctggcag
     1501 gccgttcagc ctttacgaag ccaaacgccg ggtcgctgtt cgacacatgg gcataggtgg
     1561 agcgcgatgc gtcttcatcg aacgtccagc cgaacagggc gccatagaag gccttggctt
     1621 tgccgatgtc ggcggtgtcg agggtgaagt aaccgagctg aacctgagcc atgaacgacc
     1681 ttcgctagtg cctgtgaagc ttaagcgctc acgagcagcg aaagttgcct tccgacggtc
     1741 tcacgaccgc cttcctcaat ttccctgtcc accagacggg tggggtattc gccggtgaaa
     1801 tagtggtccg tgaactgcgg gtggcggtta ttgcggccag tctcgcccat ggctttgtag
     1861 agaccgtcga ccgacaggaa cccgagcgag tcgacctcga gcagttcgcg catctcttcc
     1921 atggtcttgt tggcggcgat gagctgatca cgctcgggca tgtcgatgcc gtagaagtcg
     1981 ggccacagga tcggcgggct ggccgaacgc aggtggacct cggtagcgcc cgcgtcccgg
     2041 acggcgcgga ccagcttcac agaggtggtg ccgcgcacga tcgagtcgtc gatcagaacg
     2101 acgcgcttgc cttcgatcac cgaacggtta gggctgtgct tcatcgccac gcccttttga
     2161 cgggcgccct ggctaggctg gatgaaggtc cggcccagat agtggctgcg gatgatgccc
     2221 atctcgtagg ggatgccgct ttcctgcgca tagccgagcg cggcggggac gccggagtcg
     2281 ggcacgggca cgacgacgtc ggcctcgatc gggaactcac gggcaaggcc ctgacccatg
     2341 cgcttgcgga cgccatagac ggagcggccg ttcacgacgc tgtcggggcg cgagaagtag
     2401 acgtactcga acaggcaggg acgggcggcg cgggccggga agggcttgat cgactgcaag
     2461 ccgtgcttgt cgatgacgac gacctcgccg tgctcgacat cgcgcacgaa ggtcgcgccg
     2521 atggtgtcga gggcgcaggt ctcggacgcc agcacccagg cctcgccgat cttgccgagc
     2581 accagcggac ggatgccgag cggatcacgc gcgccgatca tctttgtgcg cgtctgggcg
     2641 acaagcgcgt agccgccctc gatgcgggcg agagcgtcga tgaagcggtc gacgatcttg
     2701 gccttacggc tgcgggcgat cagatgcagg atgacttcgg agtccgaggt cgactggaag
     2761 atcgagcctt cgccgaccag ctggttccgc agatagtgga aattggtcag gttgccgttg
     2821 tgaccgatgg cgatgccgcc ctggtcgagg tctgcgaaca tcggctggat gttgcgcagg
     2881 aacgatccac cggcggtgga gtagcgggtg tgccccacgg ctgcagcgcc gggcatccgg
     2941 gttttcagat cggtgccgcc gaaggcctcc ccgacgtggc ccatgtggcg ttccgtgtgg
     3001 aagcgggtgt cgttgacgct ggcgatgccg caagcttcct gaccgcggtg ttgcagggca
     3061 tggaggccaa gggcgacgat ggaggacgcc tcgtcttgcg cggcgcccca gacgc
//
LOCUS       DASVTM010000181         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_764798,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000181 DASVTM010000000
VERSION     DASVTM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764798"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF633_05015"
     CDS             <1..360
                     /locus_tag="VF633_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEX8470451.1"
                     /translation="IFGICLGHQMLARALGAETRKMDQGHHGANHPVKDLTTGKVEIV
                     SMNHGFTVDRDSLPDAVAETHVSLFDGTNCGIELKGRPVFSVQHHPEASPGPTDSLYL
                     FDRFADLMDAPRSADVP"
     gene            357..1322
                     /locus_tag="VF633_05020"
     CDS             357..1322
                     /locus_tag="VF633_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470452.1"
                     /translation="MSLPSAKSAYLGWLARALEPERSPLAPISGPVVGDVVLGYGTGY
                     DAADIAPFVLSLRAVFDGAVALVVDDRADLKAFLAEHGVIAVHPQHLDGWEPHAVMAR
                     FAAFDRLLGQWPEAKSVLITDVRDVIFQAEPFSPVPERLETYVEFEDGVLRDHDFNMK
                     YLRAVGGDDLAEAVADKPCVCVGTVMGPRYAMIRFCRTVMMLAAIPRSELGGAFGADQ
                     AACNLAIHMGLVEADIRPNYGRVATLGLTKPESLGWSDGRVINPDGGWSAIVHQHDRH
                     PTLATAVHARWGGGLEHRERVQKKTAAQRFEKQKRSIRRRLPELR"
     gene            complement(1330..1599)
                     /locus_tag="VF633_05025"
     CDS             complement(1330..1599)
                     /locus_tag="VF633_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470453.1"
                     /translation="MFISIARPAVEPTGPDAVAVPGSPAIAELSPRALHARLLANAAL
                     RRMRGVERKHAHRDEDAQYWFSAAPAAVRKASELRCEDRSFAPIP"
     gene            1949..>2475
                     /locus_tag="VF633_05030"
     CDS             1949..>2475
                     /locus_tag="VF633_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX8470454.1"
                     /translation="MRFDERFLEELKARLRPSDVIGRSVKLKRQGREWVGLSPFSKEK
                     SPSFFVNDDKGFFHDFSSGKHGDIISFLQEVERLSFPEAVERLAAEAGMQMPAEDPQA
                     QEREQKRQGLSDWVDLSQKWFAANLRRSAGKAAREYLEKRGLPEDQWERFGLGYAPND
                     REGLKAALVQRGAKPG"
BASE COUNT          420 a          763 c          835 g          457 t
ORIGIN      
        1 atcttcggca tctgcctcgg ccatcagatg ctcgcgcgcg cgctcggcgc cgagacgagg
       61 aaaatggatc agggccacca cggcgccaac catcccgtaa aggatctgac gacgggcaag
      121 gtcgagatcg tgtcgatgaa ccacggcttc accgtcgatc gcgacagcct gcccgacgcc
      181 gtcgccgaga cccacgtcag cctgttcgac ggcaccaact gcggcatcga actcaagggc
      241 cgtccggtgt tcagcgtcca gcatcacccc gaggcctcgc ccggcccgac cgacagcctc
      301 tatctgttcg accgtttcgc cgacctgatg gacgcgcccc gttcagcaga cgttccttga
      361 gcctgccgtc tgcgaagtcg gcctatctgg gctggctcgc acgcgcgctc gaacctgagc
      421 gcagtccttt ggcgccgatc agtggcccgg tcgtggggga cgtcgtcctc ggctacggca
      481 ccggctatga cgccgccgat atcgcgcctt tcgtgctgtc gctgcgggcg gtgttcgacg
      541 gtgcggtggc tctggtcgtt gatgaccgtg ccgacctgaa ggcgtttctc gccgagcatg
      601 gcgtcatcgc cgtccatcct cagcatctgg acggatggga gccgcacgcc gtcatggccc
      661 ggttcgccgc ctttgatcgg ctgctgggtc aatggcccga agcgaagagc gtcctgatca
      721 ccgacgtgcg tgacgtgatc ttccaggccg agccgttctc gcccgtgccc gagcggctcg
      781 aaacctatgt cgagttcgag gacggggtcc tgcgcgacca cgacttcaac atgaagtacc
      841 ttcgcgccgt cggcggcgac gatctggccg aggcggtggc cgacaagcct tgcgtctgcg
      901 tcggtacggt catgggccca cgctacgcca tgatccgctt ctgccgcacg gtgatgatgc
      961 tggccgccat tccccgctcg gaactgggtg gcgcgttcgg agccgaccaa gccgcctgca
     1021 acctcgcgat ccatatgggg ctggtcgagg cggacatccg gccgaactac gggcgagtcg
     1081 caacgctggg tctgacgaag ccggagtcgc ttggctggtc ggacgggcgc gtgatcaatc
     1141 ccgacggcgg ctggagcgcc atcgtccacc agcacgaccg gcatccaacc ttggcgacgg
     1201 cggttcacgc gcgctggggc ggggggctgg agcatcgcga gcgggtgcag aagaagacgg
     1261 ccgcccagag gtttgagaag cagaagcgct cgatccgccg gaggctgccc gaacttcgct
     1321 aagccagacc taggggattg gcgcgaagct gcgatcttcg cagcgcagct cgctggcttt
     1381 gcggactgcg gctggcgctg cgctgaacca gtactgagcg tcttcgtcac gatgtgcgtg
     1441 tttgcgctct acgccgcgca tccgacgcag ggcggcgttg gccaacaggc gcgcgtgcag
     1501 ggcgcgaggc gagagttcgg cgattgcggg ggaaccgggc acggcgacgg cgtcggggcc
     1561 agtgggttcc accgccgggc gggcgataga gataaacatc ggtcgtcttt caacggtatc
     1621 gagattgcaa cgccgaccgc gttctggccc cggatggccc gccgcattct gtggcgggtc
     1681 gtatggttaa tccacaacgt ctgtggatag ttcctttatg ccagaaaaaa tgtgcgttaa
     1741 atcaggcgct tatggtcacg cttttaacct attggttaat caaaggttaa tggcggcgct
     1801 tgagtcctgg gtaacggtgc cgctagctcg gggacaaaga tcgtccgggg gcggcacatt
     1861 tctcaacaaa aacaggcgtt tttcaccttg tccaggagcc gacgcagcgg gtctcgcttg
     1921 tgccgcttgt gcacgttaag gatcgcaggt gcgttttgat gagcgatttc ttgaagaact
     1981 gaaggctcgc cttcgcccgt ccgacgtgat cgggcggtcg gtgaagctca agcgtcaggg
     2041 acgcgagtgg gtgggcttga gtccgttctc gaaagagaag tcgccgtcgt tcttcgtcaa
     2101 cgacgacaag ggcttcttcc acgacttttc gtcgggcaag cacggcgaca tcatctcctt
     2161 cctgcaggag gtcgagcggc tgtcctttcc cgaggctgtc gagcgccttg ccgccgaggc
     2221 agggatgcag atgccggccg aggaccctca ggcgcaggag cgtgagcaaa agcgtcaggg
     2281 cctatccgac tgggtggacc tgtcgcagaa atggttcgcg gccaacctgc gccgatcggc
     2341 gggcaaggcg gcgcgcgagt atctcgagaa acgcggcctg cctgaggatc agtgggagcg
     2401 gttcggtctc ggctatgcac ctaacgaccg cgagggtctg aaggcggcgc tggtgcagcg
     2461 gggcgccaag cccgg
//
LOCUS       DASVTM010000182         4311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_603820,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000182 DASVTM010000000
VERSION     DASVTM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603820"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF633_05035"
     CDS             complement(<1..375)
                     /locus_tag="VF633_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8470455.1"
                     /translation="MADELDPIDARILDILQQDAGLSVAEVADRVGLSASPCWRRIKR
                     LEDSGLIRKRVTLLDAKLLGLDFEVYAIVKLMLPSSENLNIFEAAVSEWPEVVQCATI
                     TGREDYVLRIVTSDMHAFDKFLR"
     gene            534..1766
                     /locus_tag="VF633_05040"
     CDS             534..1766
                     /locus_tag="VF633_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEX8470456.1"
                     /translation="MTRNTQPLSLRVPEPPGRPGDAPDFSGLKLDIAGAVDRPPVDSR
                     PGQMMDMAFRLIRVLDDEGNAVGPWNPRLDSDTARRGLKAMILTRAFDDRMHRAHRQG
                     KTSFYMKCTGEEAIACAQGMILTREDMGFPTYRQQGLLIARGYPLATMMNQIYSNAAD
                     PIKGRQLPIMYSARDYGFFTISGNQGTQLPQAVGWAMASAYKGDDKIAAAWIGDGSTA
                     ESDFHSALTFASVYRAPVIINIVNNQWAISSFQGIAGGNETTFASKAIGYGLPSLRVD
                     GNDFLAVWAATQWAEERARTNQGATLIEMFTYRGAPHSTSDDPSKYRPADEQDQWPLG
                     DPIQRLRQHLTVIGEWDDERHEAALREAVEQVRQAGRESEAIGTLGQSRPSVKEMFEE
                     VYATEDWRLMEQRREVGV"
     gene            1769..2890
                     /locus_tag="VF633_05045"
     CDS             1769..2890
                     /locus_tag="VF633_05045"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8470457.1"
                     /translation="MSETYSPADQHDGIDPDMADAAEAPASVAEGTGTVPMNMIQALN
                     SALTGKMAEDPNVLSFGEDTGYFGGVFRVTDQLQQKHGLTRSFDSPINESGIVGAAIG
                     MAAYGLRPVVEIQFADYIYPAYDQIVSEAAKMRYRSGGQFSSPLVVRTPYGGGIFGGQ
                     THSQSPESLFTHIAGLKVVVPSNPYDAKGLLTSAIEDDDPVIFFEPKRLYNGPFDGWH
                     QKPVSPWKAQPEAQVPTEKYTVPIGVAKVVREGSDVTILCYGTMVWVSLAAAEHAGVD
                     AEVIDLRTLVPLDIATIEASVKKTGRCVIVHEAPKTSGFGGELSALVQERCFYHLEAP
                     IARVTGWDTPYPHAFEWEYFPGPERVATALKAVMTGGAR"
     gene            2890..>4311
                     /locus_tag="VF633_05050"
     CDS             2890..>4311
                     /locus_tag="VF633_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8470458.1"
                     /translation="MGRYVFKLPDVGEGTAEAELVGWHVKVGDHVEEDQLLADVMTDK
                     ATVELTSPVSGVVTAIHGEPGVMSAVGSPLVEFEVEGAGNAEAAEPAPAVESEVIVPF
                     ASKPEAKTEAVPEAQPAEQAAAIAAPVQASAGNYVFKLPDVGEGTAEAELVSWHVAVG
                     DTVEEDQLLAEVMTDKATVELTSPVAGVVAALYGQPGQQVPVGGPLVTFDVAGAGNVA
                     SAPPAASASAPSVTTQERRATSPDGGGSPSAKSPPVGGSTGEAGEGGKSAPKTASVTR
                     TAQTPGVRPLASPAVRNRARELGIELQFVPGSGPAGRIQHSDLDAFLARGSQAPAPTS
                     ASGSTYARAEGTTEVRIIGLRRKIAEKMAESVRRIPHITYVEEIDVTALEELRAHLNA
                     TKSKDQPKLNLLPFIARAIVVALRDQPNINATYDDEAGVLTQHAAVHLGIAAQTPNGL
                     MVPVVRHAEARDAWDTALEISRVS"
BASE COUNT          783 a         1484 c         1324 g          720 t
ORIGIN      
        1 acgcaggaat ttgtcgaagg cgtgcatgtc cgaggtgacg atgcgcagca catagtcctc
       61 gcggccggtg atggtggcgc attggacgac ctcgggccat tcgctgacgg cggcttcgaa
      121 gatgttcagg ttttcggatg acggcagcat cagcttgacg atggcgtaga cctcgaagtc
      181 caggcccagc agcttggcgt ccagcagggt cacgcgcttg cggatcaggc cggaatcttc
      241 cagtcttttg atacgtctcc agcacggcga agcggacagt ccgacccgat cggcgacctc
      301 cgcgacagac aggcccgcgt cctgttgcag gatgtccaga attctcgcgt cgatgggatc
      361 gagttcgtcc gccaaggcgt ttctccgttt gttattatgg cgcaataaaa taccctgaaa
      421 ccacctaatg caaggcgaag ttcggtaagt cttgatctgc cacgtctgct atcaggcgct
      481 ataaaggcaa atgcggtcgg atcaaccagg cggcggccta gaggcaggac gaaatgacca
      541 ggaataccca gcccctgtcc ctgcgtgtcc cggagcctcc ggggcgtccc ggtgatgcgc
      601 ccgacttcag tgggctgaag ctggatatcg ccggcgctgt cgatcgtccg cccgttgatt
      661 cccgtccagg ccagatgatg gatatggctt tccgcctgat ccgggtgctg gatgacgagg
      721 gcaacgcggt cggcccatgg aacccgcgtc tcgactccga cactgcccga cgcggcctca
      781 aggccatgat cctgacccgc gccttcgacg accggatgca tcgcgcccac cgtcagggca
      841 agaccagctt ctatatgaaa tgcacgggcg aagaggccat cgcctgcgcc caggggatga
      901 tcctgacccg cgaggacatg ggttttccaa cctatcgcca gcaggggctt ctgatcgcgc
      961 gtggctatcc gctggcgacc atgatgaacc agatctattc caacgccgcc gacccgatca
     1021 aaggtcgcca gctgccgatc atgtattcgg ctagggacta tggcttcttc accatcagcg
     1081 gcaatcaggg cacccaactg ccgcaggccg tgggttgggc tatggccagc gcctacaagg
     1141 gcgatgacaa gatcgcggca gcctggatcg gcgacggctc gaccgccgag agcgacttcc
     1201 actcggcgct gacgttcgcc tcggtctatc gcgcgccggt gatcatcaac atcgtcaaca
     1261 accagtgggc catctcgtcg tttcagggca tcgctggcgg caacgagacg accttcgcgt
     1321 ccaaggccat cggttatggc ctgccgtccc tgcgtgtcga cggcaacgac ttcctcgccg
     1381 tctgggccgc gacccagtgg gccgaggaac gcgcccgcac caatcagggc gcgaccctga
     1441 tcgagatgtt cacctatcgc ggcgcgcctc actcgacctc ggacgatcca tcgaaatacc
     1501 ggcccgcaga tgagcaggac cagtggccgc tcggcgaccc gatccagcgc ctgcgtcagc
     1561 acttgaccgt catcggcgag tgggatgacg aacgccatga ggctgcgctc cgtgaagctg
     1621 tcgaacaggt ccgtcaggcg ggccgtgaat ccgaggccat cggcacgctc ggtcagtcgc
     1681 gtccttcggt gaaggagatg ttcgaggagg tctatgcgac cgaggactgg cgcctgatgg
     1741 aacaacgccg cgaggttggg gtctgaacat gagcgagacc tattcccccg ccgaccagca
     1801 cgacggcatc gatcccgaca tggccgacgc tgccgaagcc cctgcgtccg tcgccgaagg
     1861 aaccggtacg gttccgatga acatgatcca ggccctgaac tcggccctga ccggcaagat
     1921 ggctgaggat cccaacgtcc tctccttcgg tgaggatacg ggttatttcg gtggggtgtt
     1981 ccgcgtcacc gaccagcttc agcagaagca cggcctgacc cgcagcttcg actcgccgat
     2041 caacgagagc ggcatcgtcg gcgccgccat cggcatggcc gcctatggcc tgcgcccggt
     2101 cgtcgagatc cagttcgccg actacatcta tccagcttac gaccagatcg tctccgaggc
     2161 ggccaagatg cgctaccgct cgggcggcca gttctccagc ccgctcgtgg tccgcacccc
     2221 ttacggcggc ggcatcttcg gcggccagac gcactcacaa agccccgaaa gcctgttcac
     2281 tcacatcgcc ggcctcaagg tcgtggtgcc gtcgaacccc tatgacgcca agggtctgct
     2341 gacctcggcc atcgaggacg acgacccggt catcttcttc gagcccaaac gcctctacaa
     2401 cggacccttc gacggctggc accagaagcc ggtctcgccg tggaaggccc agcctgaagc
     2461 ccaggtcccg accgagaaat acaccgtgcc gatcggcgtg gccaaggtcg tgcgcgaagg
     2521 ctcggacgtg accatcctgt gctacggcac catggtctgg gtctcgctgg ccgcagctga
     2581 acacgcaggc gtggacgccg aggtcatcga cctgcgcacg ctcgtgccgc tcgacatcgc
     2641 gaccatcgag gccagcgtca agaagaccgg ccgctgcgtc atcgtccacg aagcgcccaa
     2701 gacctcgggc ttcggcggcg agctttcggc tctggttcag gagcgctgct tctatcatct
     2761 ggaggcgccc atcgcccgcg ttaccggctg ggacacgccc tatccgcacg ctttcgagtg
     2821 ggaatatttc cccggacctg aacgggtcgc cacggctctg aaagccgtca tgaccggagg
     2881 cgcacgctga tgggccgtta tgtcttcaaa ctgcccgacg tgggcgaagg caccgccgag
     2941 gctgaactgg tcggctggca cgtcaaggtc ggcgaccatg tcgaggagga tcaactcctc
     3001 gccgatgtga tgaccgataa ggccaccgtc gagctgacct cgccggtctc cggcgtcgtc
     3061 actgccatcc acggcgaacc cggtgtcatg tccgccgtcg gctcacctct ggtcgagttc
     3121 gaggtcgagg gtgcgggtaa tgccgaagcc gctgaaccag ctcctgccgt cgagtccgag
     3181 gtgatcgttc cgttcgcctc gaagcccgaa gcaaagactg aagctgtccc cgaagctcaa
     3241 ccggctgagc aggctgcagc catcgctgcg cctgtccagg ctagcgcagg caactacgtc
     3301 ttcaaactgc ctgacgtggg cgagggcacg gcggaggccg agctggtctc gtggcacgtc
     3361 gcagtcggcg acacggtcga ggaagaccag ctcctcgccg aagtcatgac cgacaaggcc
     3421 accgtcgaac tgacctcgcc ggtcgccggc gtcgtcgccg ccctctacgg ccaaccgggc
     3481 cagcaggtcc ccgtcggtgg tccgctggtg acgttcgatg tcgcgggcgc cggtaatgta
     3541 gcctctgctc ccccggcggc gtccgcttcg gccccctccg tcacgacgca agaacgccgc
     3601 gccacctccc ccgacggggg aggatcgcca tccgcaaagt ctccccccgt cggggggagt
     3661 accggcgaag ccggggaggg gggcaagtcc gcacccaaaa ccgcttccgt cacccgcacc
     3721 gcacaaaccc ccggcgtccg cccgctcgca tccccggccg tccgcaaccg tgcccgcgaa
     3781 ctcggcatcg agctgcagtt cgtccccggc tccggccccg ccggtcgtat ccagcattcc
     3841 gacctcgacg ccttcctcgc gcgcggatcc caagccccgg ccccgacgtc cgcgtccggc
     3901 tcgacctacg cccgcgccga aggaacgacc gaggttcgca tcatcggtct gcgccgcaag
     3961 atcgcggaga agatggccga gagcgtccgg cgcatccccc acatcaccta tgtggaggag
     4021 atcgacgtca cggctctgga ggagcttcgc gcccacctga atgcgacgaa gtcgaaggac
     4081 cagcccaagc tcaacctgct gcccttcatc gcccgcgcca tcgtcgtggc cctgcgcgac
     4141 cagcccaaca tcaacgccac ctatgacgac gaggccgggg tcctgacaca gcacgccgcc
     4201 gtgcatctgg gcatcgcggc ccagacgccg aacggcctga tggtgccggt ggtccgtcac
     4261 gccgaggcgc gcgacgcctg ggatacggcg ctggaaatct cccgcgtctc g
//
LOCUS       DASVTM010000183         8412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_975087,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000183 DASVTM010000000
VERSION     DASVTM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975087"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1516
                     /locus_tag="VF633_05055"
     CDS             239..1516
                     /locus_tag="VF633_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX8470459.1"
                     /translation="MRFDYRLFLIACVAACAPMVPTPPMGVPQPGPTPQPAPSRPTPQ
                     QPNAAAPVGAFDQPGFEGWKRGFLDRMGGARRAAYEQQLANLTPDPDVIRLDSNQPEF
                     SRPTGAYVANAVSSARIAEARSKIDVVPWSVTQRFGVPAEILVSIWAQESSFGKVQGN
                     NDVIRSLATLAYEGRRREWAEGQLRDALNIVIDGRRERSGLKGSWAGAMGQTQFMPDN
                     YLRLGIDQNEDGKVDIWGTDVDALASAANLLAQAGWKRGQSWGYEVVVPSGFDYALAD
                     GAAQPWSFWRRHGVTLARGGGMSDQEAAENAVLLLPQGARGPAFIALPNHFVIRRYNN
                     SVSYALAIGLTADGAAGKPGLVAAWPNDPAMSRDQRIGAQAALTRLGYDTGGIDGVVG
                     ANTRAALKRWQIANGRIADGYLTAALAAELSGR"
     gene            1733..2986
                     /locus_tag="VF633_05060"
     CDS             1733..2986
                     /locus_tag="VF633_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX8470460.1"
                     /translation="MASDVSPSIVLVVEDELMLRMRAVDIVQDAGFESIEAISADQAI
                     EILESRDDISVLFTDIQMPGSMDGLKLAHVTHARWPHIKIILVSGQIAVTEADKPYDS
                     KFFPKPLEIEQMILELQDMVGAGALKVLPSLSAENGSLTKENRELRYQLQQAGVHAKA
                     LLLKTVLDTEKQQLIIDENQPFQDALTTENDSLKLALEQAGIDANALLIQAGIEAEER
                     DAADKLQNLIHAELHHRIKNMLATVGAITDQSLRTATSMAHARSAIDGRFAALARAHD
                     LLTRSSWENATINSTVRSAIEPFDQGGERFIISGEDIRITSSSVIAFAMTLNELCTNT
                     TKFGALSLPSGQVDISWRVDGGRVHFDWAESGGPAVTEPTRKGFGTRMITALGQQLKG
                     QVALDYNSNGFVYSLNVPVEALVSK"
     gene            3266..3508
                     /locus_tag="VF633_05065"
     CDS             3266..3508
                     /locus_tag="VF633_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470461.1"
                     /translation="MSDPTSDGNRAILAGFLAFLSIGLLLMAAFALFRVIVPLILEAH
                     FFGSVFVASVAGLFGTLGLGVLAVFLFKRIAGMIRT"
     gene            3669..4526
                     /locus_tag="VF633_05070"
     CDS             3669..4526
                     /locus_tag="VF633_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8470462.1"
                     /translation="MSRAARPGSRRQSSGPTPGFSPPNCGPGWHWEGLGEKIIKYPTR
                     QRVYSYTREGNADGQENEEIGFADQTGLPMTADVNMTVKVADNKTADLYAKYRLSFDD
                     LLDNPIRNDVRSFIAQEAEKVPVSCNLQAQQPVEAGAQPAPTNCTGSLMGAGRQAVIQ
                     RAYANLQRKWAAEGVEISNLQWVGTIRYPESITTAIQARTATEQRTLAAQQKEAEARA
                     NANALIETARGEAESIRLRGDALRANPQLIDQIYAQRSQGLCPPRASTCIIGQTPTTM
                     IPATGQQAE"
     gene            complement(4723..6132)
                     /gene="cysS"
                     /locus_tag="VF633_05075"
     CDS             complement(4723..6132)
                     /gene="cysS"
                     /locus_tag="VF633_05075"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX8470463.1"
                     /translation="MTLSLYDTLHREKRVFTPRDPDRITLYVCGPTVYDYAHIGNARP
                     PVVFDVLTRLLRREYGSDSVIYARNVTDVDDKINAKAHLEGVAIGEVTARYEAAYLAD
                     MAALNVTPPDLTPHVTEHMDAIIAQIGQIVDAGCAYAAEGHVLFDVSSYPAYGALSGR
                     NLDDMIAGARVEVAPYKKNPHDFVLWKPAKENEPSWRSPWGDGRPGWHIECSAMIEET
                     LGLPIDIHGGGIDLVFPHHENEIAQGVCAHGHAHSDAAPTEYSRYWMHNGFLTVDAEK
                     MSKSVGNVLLLHDLVQNIPGEVVRWALLSAHYRQPLDWNQSLLDQSKKNLDRLYGALR
                     RCADVEAKGDMPSPEFLEAISDDLNTPGAMSKLFALSSEIERAMTAGDLQAVAIAKAE
                     LLASGALMGVLLSSPDQWFEGGADDALKTEVEGLLAERITARAAKDWPAADRIRDRLT
                     ELNVVVMDSPTGATWRLKE"
     gene            6268..6885
                     /gene="folE"
                     /locus_tag="VF633_05080"
     CDS             6268..6885
                     /gene="folE"
                     /locus_tag="VF633_05080"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX8470464.1"
                     /translation="MSSIPAKPVLVPGNDQLLKPSREQALEAVRTLIAWAGDDPTRPG
                     LIDTPKRVVDAYAEWFEGYDADPAKELSRTFEDVTGYDDMVVLRQIEVESHCEHHMAP
                     FLGKAFVAYMPGEKVVGISKLARVVEIFAKRLQNQETLTNDIVEAIESHLAPKGVAVL
                     IDAEHQCMTTRGVHHRHVSTITTRFTGAFKSDPALVDRFLKLARG"
     gene            7044..7487
                     /locus_tag="VF633_05085"
     CDS             7044..7487
                     /locus_tag="VF633_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8470465.1"
                     /translation="MGSKLAGPGGQGGKTIVQNSDINVTPFVDIMLVLLIIFMVSAPL
                     ATVSIRLDLPPATPPTGEKAPDPVYITIQESGSIFIAEKQTTIETLYADVCTAVGNPT
                     GKGCTEERVFVRAQPEVKYNQFMEVMNDLQAHGFFKVGLLNEDIE"
     gene            7616..8038
                     /locus_tag="VF633_05090"
     CDS             7616..8038
                     /locus_tag="VF633_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8470466.1"
                     /translation="MPTRIQRPGLKAVLQNADINVTPFVDIMLVLLIIFMVSAPLATV
                     SLKLDLPPPIAEQFFKNDPVYVTVQASGAVFVADKASTMEGLSGDVCTALGGGDCHDE
                     RIFVRSEPDVPYRQFMAVMNDLHGHGFVKVGLLNEDIE"
     gene            8152..>8412
                     /locus_tag="VF633_05095"
     CDS             8152..>8412
                     /locus_tag="VF633_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470467.1"
                     /translation="MISTLMVALALAQQPVCSPPPHAELLWANPATRFGFIGETHGTN
                     ETPAAFAEMVCEASATRPVIVALELPSVIQPDLEAWMASAGGE"
BASE COUNT         1576 a         2685 c         2674 g         1477 t
ORIGIN      
        1 aggcctggcc tccgagttga acggagggtg tatgggcttt cgggcccatc gcgtagcccc
       61 tccgccacca ggccgcatca gggagatgac agaatgtcgc acttccattg cgatccccca
      121 aacggagatg gtcaatgcgc cattcatact cgcgatgtca tgtgacggga tgcaacgctt
      181 ctcgcctgta gcgcggttgt gattgcggtt tcgcctcacg ggacactagc ctgacgtcat
      241 gcgcttcgat taccgactct tcctgatcgc ctgcgtggcc gcctgtgcgc ccatggtccc
      301 cacgcctccg atgggcgtgc ctcagcccgg gccgacgccc cagccggcgc cgtcgcggcc
      361 cactccccag cagcctaatg ccgctgcccc ggtcggcgcc tttgaccaac cgggcttcga
      421 aggttggaag cgcggtttcc tcgaccgcat gggcggcgcc cggcgcgccg cctatgagca
      481 gcagctggcc aatctgacgc ccgaccccga cgtcatccgg ctggacagca atcagcccga
      541 gttcagccgc cccaccggcg cctatgtcgc caacgccgtg tcatccgccc gcatcgccga
      601 agctcgcagc aagatcgatg tcgtgccatg gagcgtgacc cagcgctttg gcgtcccggc
      661 cgagatcctc gtctcgatct gggctcagga gtccagcttc ggcaaggtcc agggcaacaa
      721 cgacgtcatc cgctcgctgg ccaccctcgc ctatgaaggc cgccgccgcg aatgggccga
      781 gggccagctg cgcgacgccc tgaacatcgt tatcgacggc cgccgcgaac gctccggcct
      841 caagggcagc tgggccggcg ccatgggcca gacccagttc atgcccgaca actacctacg
      901 cctcggcatc gaccagaacg aggacggcaa ggtcgacatc tggggcaccg acgtcgacgc
      961 cctcgcctcc gccgccaacc tgctggccca agccggatgg aagcgcggcc agagctgggg
     1021 ctatgaggtc gtggtgccct caggcttcga ctacgcgctg gccgacggcg ccgcacagcc
     1081 gtggagcttc tggcgccgac acggcgtgac cctcgcgcgc ggtggcggca tgtccgatca
     1141 ggaagccgcc gagaacgccg tcctgttgct gccacaaggc gcgcgcggcc cggccttcat
     1201 cgccctgccc aaccatttcg tcatccgccg ctacaacaac tcggtcagct atgcgctggc
     1261 tatcggcctg acggcggacg gcgcggcggg caagcccgga ctggtcgccg cctggcccaa
     1321 cgaccccgcc atgtcgcgcg accagcgtat cggcgcacag gccgccctga cccgtctggg
     1381 ctacgacaca ggcggcatcg atggcgtcgt cggcgccaac acacgggcgg ccctcaagcg
     1441 ctggcagatc gccaacggaa ggatcgccga cggctatctg acggcggccc tcgcggccga
     1501 actgtcgggg cgataggggc ggaacgcacc cggaaaatac ctagtgtaaa aggtgcaagc
     1561 caaaggggtt tgcaacgaaa ccctatgact ggagagtata aatccgctcg gaaggtatga
     1621 actaacccgc attgtttcag ccagccaagc ttgacagtcc agacaggcag gcccagcgga
     1681 gcggtcgggt cggcgctgtc tttcgttaag gccattcccc taggagttgg tgatggcctc
     1741 agatgtttct ccgtcgatcg tgcttgttgt cgaagacgaa ctcatgctgc gcatgcgtgc
     1801 cgtcgacatc gtccaggacg cggggttcga gtcgatcgaa gcgataagcg ccgatcaggc
     1861 catcgagatt cttgagtcgc gcgatgatat ttccgtgctg ttcaccgaca tccagatgcc
     1921 gggaagcatg gacggcctga agctggccca tgtgacgcat gcccgttggc cccatatcaa
     1981 aatcatcctg gtgtcggggc agatcgcggt caccgaggcg gataagccgt atgacagcaa
     2041 gttctttccc aagccgctcg aaatcgagca gatgattctg gaactccagg acatggtcgg
     2101 cgcaggcgca ctgaaggtgt tgccttccct gtccgcggag aacggttcgc ttaccaagga
     2161 gaaccgggaa cttcgttacc agctccagca ggcaggcgtc cacgcgaagg ccctgctgtt
     2221 gaagaccgtg ctcgacacgg aaaaacagca gctgatcatc gacgaaaacc aaccctttca
     2281 agacgccctc acgaccgaga acgacagcct gaaacttgcg ctcgagcagg cgggcatcga
     2341 cgctaatgca ttgttgattc aggctggtat cgaagccgaa gagagggatg ccgccgacaa
     2401 gctgcagaac ctgatccatg ccgaattgca ccaccggatc aagaacatgc tcgccaccgt
     2461 gggggccatc accgatcaaa gcctgcgcac cgcaaccagc atggcccatg caaggtccgc
     2521 gatcgatgga cggttcgcgg cgctggcgcg cgcacacgat ctgctcaccc ggtcgagctg
     2581 ggaaaacgcg acgatcaaca gcaccgtgcg aagtgcgatc gagccgttcg atcagggggg
     2641 cgaacggttc atcatctccg gcgaggatat ccggatcacg tccagctcgg taatcgcgtt
     2701 cgcgatgacc ttgaacgagc tttgcaccaa cacgacgaag ttcggggccc tgtcgcttcc
     2761 atcaggtcag gtcgacattt cctggagggt ggatggcgga cgtgttcact tcgactgggc
     2821 tgaatcgggc ggtcccgctg tgacggagcc cacccgcaag ggtttcggaa cccgaatgat
     2881 cacggcgctc ggccagcagc tcaagggcca ggtggctctc gactataact caaacggctt
     2941 cgtctattcg ctgaacgtgc cggtcgaggc gctggtcagc aaatagagcg ggccgcgcaa
     3001 ggcgatgccc ctcgcgctgc gttggcgagc gggacaggcg gcgcgctgtc actccgccgg
     3061 tttcaggggt gcgaacatga aggaactccg gcctctgatg atatcccgcg ttacaaacga
     3121 tggacgaagc cctgtggcaa ggcgccgcag cggtccggca aacggccctt agcgtggtcg
     3181 cttctggcgc ggctcaaaac tttcccgcta tgatcgaaat gttgtgggcg acaggcgccg
     3241 acgcgagcac gacaggggga gacggatgag cgatccgaca agtgacggca accgggcgat
     3301 cctggccggc tttctcgcct tcctgtcgat cgggctgctg ctgatggcgg cgttcgccct
     3361 gttcagggtc atcgtgccgc tgattcttga ggcgcatttc ttcgggtcgg tgttcgtggc
     3421 ctcggtagcg ggtctcttcg gtacgctggg gctgggtgtg ctggcggttt ttctattcaa
     3481 acgtatagcg gggatgatcc ggacctaggt ccgacatcaa cgcgggggaa cgacatcgat
     3541 gactattcag ggaaccgcgc aaaagctcgg caggggcggg ccggcggcgt tgagactgac
     3601 tgcgacggtg gccttgctgg ccatcgttct ggcgtgcggc agcctcgcgg tcacgtcgtg
     3661 tagcgtcact gtcgagagcg gcgaggccgg gatcaagacg acaaagttcg ggcccaaccc
     3721 cggggttcag cccaccgaac tgcgggcccg gctggcactg ggaaggtctg ggcgagaaga
     3781 tcatcaaata tccgacccgc cagcgcgtct attcctacac ccgcgaagga aacgctgacg
     3841 ggcaggagaa cgaggagatc ggcttcgccg accagaccgg cctgccaatg acggcggacg
     3901 tgaacatgac ggtcaaggtc gcggacaaca agacggcgga cctgtacgcc aaataccgtt
     3961 tgagcttcga cgacctgctc gacaacccga tccgcaacga cgtccgctcg ttcatcgcgc
     4021 aggaggccga gaaggtgccc gtatcctgta acctgcaggc ccagcagcca gtcgaggcgg
     4081 gcgctcagcc ggcgccgacc aactgtaccg gaagcctgat gggtgccggt cgtcaggctg
     4141 tgatccagcg cgcctatgcc aacctgcaac gcaaatgggc agccgagggc gtcgagattt
     4201 cgaacctcca gtgggtcggc accatccgct atcccgagag catcacgacg gcgattcagg
     4261 cgcggaccgc gaccgaacaa cgcacgctgg cggctcagca gaaggaagcc gaggctcgcg
     4321 ccaacgccaa tgcgctgatc gaaacggcgc gcggcgaggc ggagtcgatc cggctgaggg
     4381 gcgacgcttt gcgggcgaac ccgcagctga tcgatcagat ctatgcgcag cgttcacagg
     4441 ggctgtgccc tccccgcgcc tcgacctgca tcatcggcca gacgccgacg acgatgatcc
     4501 cggcgacggg tcagcaggcg gagtagagcg ttctcctccc tggcgcgcag cgatggggag
     4561 gtggcgcgac gccgcttgcg tcgtgacgga gggggcgacc gggtggcgcc ggtagcgcgt
     4621 cagccagccc tcactccgac cccggtgtcg ccccctccgt ctcgtcggct atcgccgcga
     4681 tccacctccc cgttcgcgac gcgaatggag aggtgaagcc agctactcct tcagccgcca
     4741 ggtcgcgccg gtcggactgt ccataaccac gacgttcaac tcggtcaggc ggtcgcggat
     4801 tcggtcggcg gcgggccagt ccttggcggc gcgggcggtg atacgctcgg ctagcaagcc
     4861 ttcgacctcg gtcttgagcg cgtcgtcggc gccgccttcg aaccactgat cggggctcga
     4921 cagaagcacg cccatgaggg cgccggaggc cagcagttcg gccttggcga tggcgacggc
     4981 ctgaagatcg ccggcggtca tggcgcgctc gatctcgctc gacagggcga acagcttgga
     5041 catggcgccc ggcgtgttca ggtcgtcgga gatggcctcg aggaactcgg gcgacggcat
     5101 gtcgcccttg gcctcgacat cggcgcagcg acgcagagcg ccgtacaggc ggtcgaggtt
     5161 cttcttcgac tggtccagca gcgactggtt ccagtcgagc ggctgacgat agtgggcgct
     5221 cagcagcgcc cagcggacga cttcaccggg aatgttctgc accagatcgt gcagcagaag
     5281 gacgttgccg accgacttgg acatcttctc ggcgtcgacg gtcaggaagc cgttgtgcat
     5341 ccagtatcgg ctgtattcgg tcggtgcggc gtcggagtgc gcgtggccgt gggcgcagac
     5401 gccttgcgcg atctcgttct cgtggtgcgg gaaaaccagg tcgatgccgc cgccgtggat
     5461 gtcgatcggc aggcccagcg tctcctcgat catggccgag cattcgatat gccagcccgg
     5521 acgcccatcg ccccagggcg accgccagga cggctcgttc tccttggccg gcttccacag
     5581 gacgaagtcg tgcgggttct tcttgtacgg tgcgacctcg acgcgggcgc cggcgatcat
     5641 gtcgtcgagg ttgcggcccg acagcgcgcc gtaggccggg tacgacgaga cgtcgaacag
     5701 cacgtgaccc tcggcggcat aggcgcagcc ggcgtccacg atctggccga tctgggcgat
     5761 gatggcgtcc atatgctcgg tgacatgggg cgtcaggtca ggcggcgtga cgttcagcgc
     5821 ggccatgtcg gccagatagg cggcctcata gcgggcggtg acctcgccga tggcgacgcc
     5881 ttccagatgc gccttggcgt tgatcttgtc gtccacgtcg gtgacgttgc gggcgtagat
     5941 gacgctgtcg gagccatact cacggcgcag caaccgggtc aggacgtcga acaccactgg
     6001 cggccgcgca ttgccgatat gggcatagtc atagacggtc ggcccgcaca catagagggt
     6061 gatgcggtct ggatcgcgcg gcgtgaacac ccgcttttca cggtgcaggg tgtcatagag
     6121 ggaaagcgtc atagttcgga ggcggccttg tcgttttctt gcagggacga gattggctcc
     6181 catatagccg ccaagctgat agatagtcac cgaagactga cttttcgcga ccccaatcgc
     6241 cgcgccgccc gttcgagacc gattttcatg agttcaatcc ctgcaaaacc tgttctcgtt
     6301 cccgggaacg accagctcct gaagcccagc cgggagcagg ctctggaggc tgtgcggacc
     6361 ctgatcgcgt gggccggcga cgatcccaca cgccccggac tcatcgacac gcccaaacgg
     6421 gtggtcgatg cctatgccga atggttcgag ggctacgacg ccgacccggc caaggaactg
     6481 tcgcgcacct tcgaggacgt gaccggctat gacgacatgg tcgtgctgcg tcagatcgag
     6541 gtcgagagcc actgcgagca ccatatggcg ccgttcttgg gcaaggcgtt cgtcgcctat
     6601 atgccggggg aaaaggtcgt cggaatctcc aagctggctc gggtggtcga gattttcgcc
     6661 aagcggctgc agaaccagga aaccctgacg aacgacatcg tcgaggccat cgaatcacat
     6721 ctggcgccca agggcgtcgc agtactgatc gacgccgaac accagtgcat gaccacccgc
     6781 ggagtgcatc atcgccacgt ctcgaccatc accacccgct tcaccggcgc gttcaagtcc
     6841 gatccggctt tggtcgatcg cttcctgaaa ctcgcccgcg gctgagtttc agcggaaaat
     6901 tgcatcgggc cgaaattcct taccaaaagg aacctccggc gcgttgagac cgctttacaa
     6961 gcgacgccgg gaacgccaag acacgggtct gacctttcaa ccgcagcctc gcagagcggg
     7021 gcatcgacgg agaaatatag catatgggat ccaaactcgc aggtccgggc ggccagggcg
     7081 gcaagacgat cgtccagaac tcggacatca acgtcacgcc tttcgtcgac atcatgctcg
     7141 tgctgctgat catcttcatg gtgtcggcgc cgctcgccac cgtttcaatc cgcctcgacc
     7201 ttccgccggc gaccccaccc acgggtgaga aggcgccgga ccccgtctac atcaccatcc
     7261 aggagtcggg ctcgatcttc atcgccgaga agcaaacgac gattgaaacc ctgtacgccg
     7321 atgtctgcac cgccgtcggc aacccgaccg gcaagggctg caccgaagaa cgcgtgttcg
     7381 tccgcgccca gcctgaagtg aaatacaacc agttcatgga agtcatgaac gacctgcaag
     7441 cccacggctt cttcaaggtc ggcctcctga acgaagacat cgagtagggt tcagacccgt
     7501 tcccaaagaa gaggccgcgt tccgaaagga gcgcggcctt ttctttgcct gaatgtgatc
     7561 cgcagatgga cgtgcagata acgtggttct gcccctcatc gatgcggaga tcgctatgcc
     7621 gactcgtatt caacgaccgg gcctcaaggc cgtgcttcag aacgccgaca tcaacgttac
     7681 gccgttcgtg gacatcatgc tggtgctgct gatcatcttc atggtctcgg cgccgctggc
     7741 caccgtgtcg ctgaagctgg acctgccccc tccgattgca gagcaatttt tcaagaacga
     7801 cccggtctat gtgaccgttc aggcctctgg cgcggtgttc gtcgcggaca aggcctcgac
     7861 gatggagggg ctgtccggcg acgtctgcac cgcgcttggc ggcggcgatt gccatgacga
     7921 gcgcatcttc gtccgcagcg aacccgacgt tccttatcgg cagttcatgg ccgttatgaa
     7981 cgacctccac ggccacggct tcgtcaaggt cggcctgttg aacgaggaca ttgaatagcg
     8041 ctccaacctt tcgaggaagg gtccgcgtcc cccggaccgt ggaccctttt cttgccacga
     8101 cttcgccgtt catctgatcg accgtcgcaa ccttgaggtt tggaggggcc tatgatttca
     8161 actttgatgg tggcgctggc tttggcgcag cagccggtgt gttcgccgcc cccacatgcg
     8221 gagctgctgt gggcaaatcc ggcgacgcgt ttcggcttta tcggcgagac ccacggcacc
     8281 aacgagacac cggcggcatt cgccgagatg gtctgcgagg caagcgcgac acggccagtg
     8341 atcgtggcgt tggagttgcc atctgtcatc cagccagatc tcgaggcctg gatggcctct
     8401 gccggtgggg aa
//
LOCUS       DASVTM010000184         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_603864,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000184 DASVTM010000000
VERSION     DASVTM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603864"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1456)
                     /gene="lpdA"
                     /locus_tag="VF633_05100"
     CDS             complement(53..1456)
                     /gene="lpdA"
                     /locus_tag="VF633_05100"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8470468.1"
                     /translation="MTQTQQIKTKVLIIGAGTGGYVAGIRCGQLGLDTVLVDGGDGLG
                     GTCLNVGCIPSKAIIHAAGKFETVVKAAGDGTLGITASEPAIDLSKTVAWKDGIVRKL
                     NNGVAALLKKSKVKVIKGWATFADAKTCRVETDDGPITITAEHVILATGSEPVELPFL
                     PFGGDVMSSTEALSLDAVPNRLVVVGGGYIGLELGIAFRKLGAEVAIVEMADRILPLY
                     DKALTEPVMKWLTSHGVEVHLGARAGGFGDGKLNVTTRDGQPLQLDADKVLVTVGRRP
                     RTKGWGLENMGVAMAGPFVKIDDRCATSMRNVWAVGDLTGEPMLAHKGSAQGEIVAEI
                     ISGKDRRFDPVTIAAVCFTEPEIVSAGLGPLDVEGRDDIITAVFPLAAIGRALAIEAG
                     EDGGFVRVLASKTDHRLLGVQAVGQHVAELSNSFAQMLEMGAVLEDVAGVIHVHPTLG
                     EAFHEATLRALGHAIHI"
     gene            complement(1929..>2251)
                     /locus_tag="VF633_05105"
     CDS             complement(1929..>2251)
                     /locus_tag="VF633_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX8470469.1"
                     /translation="ISRVSGAAKDGSAKREELMGSTITITSLGTLGGVVHTPIINHPE
                     VAIVGPNKIAERVVVRDGQMVIRKMMNLSSSFDHRIVDGHDAAVFVQRIKGLLEQPAT
                     LWMG"
BASE COUNT          391 a          752 c          704 g          404 t
ORIGIN      
        1 gctccgcgag ccgatcggta gcgctcttct aagggcgcct tcccgccctt cgctaaatat
       61 ggatcgcgtg gccaagggcg cggagcgtcg cttcgtggaa cgcctcgccc agcgtcgggt
      121 ggacatggat cacgcccgcc acatcctcca gcaccgcacc catctcgagc atctgggcga
      181 agctgttgga cagttcggcg acgtgctggc ccacggcctg aacgccgagc aggcgatgat
      241 cggtcttcga cgccagcacc cggacgaagc ccccgtcctc gcccgcttcg atcgccaggg
      301 cgcggccgat ggcggcgagc gggaagacgg cggtgatgat gtcgtcgcgg ccctcgacat
      361 cgagcggccc cagaccggcg gagacgattt ccggctcggt gaaacagacg gcggcgatgg
      421 tcaccgggtc gaaacggcgg tctttacctg agatgatttc ggcgacgatt tcgccctgcg
      481 ccgaaccctt gtgggccaac atgggttcgc cggtcaggtc gcccaccgcc cagacgttgc
      541 gcatcgaggt ggcgcaacgg tcgtcgatct tcacgaacgg ccctgccatg gcgacgccca
      601 tgttctcgag gccccagccc ttggtgcggg gacgacggcc gacggtgacc agcaccttgt
      661 cagcgtccag ttgcagcggc tggccgtccc tggtggtgac gttcagcttg ccgtcaccga
      721 atccgcccgc acgagcgccg agatgcactt cgaccccgtg gctggtcagc cacttcatca
      781 cgggctccgt cagggccttg tcatagaggg gcaggatgcg gtcggccatc tcgacgatag
      841 ccacctcggc gcccagcttg cggaaggcga tgcccagctc cagcccgata tagccgccgc
      901 cgacgacgac cagcctgttc ggcacggcgt ccagcgacag cgcctccgtg gacgacatga
      961 catcgccgcc gaaaggcagg aatggcagtt cgaccggctc cgagccggtg gccaggatga
     1021 cgtgttcggc ggtgatggtg atcgggccat catcggtctc gacgcggcag gtcttggcgt
     1081 cggcgaacgt cgcccagccc ttgatgacct tgaccttgga cttcttcagc agggcagcga
     1141 cgccattgtt cagcttacgg acgatcccgt ctttccaggc gacggtcttg gacagatcga
     1201 tggccggctc ggacgccgtg atgcccagcg taccgtcgcc ggccgccttg accaccgtct
     1261 cgaacttgcc tgccgcgtgg atgatcgcct tggacgggat gcagccgacg ttcaggcacg
     1321 tcccgccgag accgtcgccg ccatccacca gcacagtgtc gaggcccaac tgaccacagc
     1381 gaatgcctgc gacatagccg ccagtgcctg cgccgatgat caggactttg gttttgatct
     1441 gctgtgtctg ggtcatgagt ccggctagtt tcgataagcg cgcgatcgcg ccaagctgta
     1501 gagtcgacgg ccgcggatac tgtctctccg cgcgacctcc aagatttcga tccgccacca
     1561 agatcgtcgc ctcgccacaa cgatcaccgg ctgccccggc agaatacgtc gtaccccgtc
     1621 cagggaataa attggctctc tcccgcagct cgtctcgtag cggggggatg tttgagcata
     1681 gacaggggcc cgaccacccc ggatcgtctg ctcaccccgg attgcgtatc gctctcgggt
     1741 aagaccacca gagttccaga gcttgccgaa atagaccagg tctgcgccat cgatcatggc
     1801 gttctcgcgc tcctgttcca actgccgcgt aagttcttcg gacgttacga gaatcgtgca
     1861 ggcgaaaaca ggcgtcgccc ccacgatcaa ggcgaccgca gacgcaatag cgacgccccg
     1921 catcatgttc accccatcca cagcgtggcg ggttgctcaa gcaggccctt gatcctctgc
     1981 acgaagaccg ccgcgtcgtg gccgtcgacg atgcggtgat cgaaggatga ggacaggttc
     2041 atcatcttgc ggatgaccat ctggccgtcg cggacgacga cccgttcggc gatcttgttg
     2101 ggaccgacga tggcgacctc ggggtgattg ataatcgggg tgtgaaccac cccgcccagc
     2161 gtgcccagcg aggtgatggt gatggtcgaa cccatcagtt cctcgcgctt ggccgacccg
     2221 tccttggccg cgcccgagac gcgggagatt t
//
LOCUS       DASVTM010000185         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_711109,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000185 DASVTM010000000
VERSION     DASVTM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711109"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF633_05110"
     CDS             <1..409
                     /locus_tag="VF633_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8470470.1"
                     /translation="VRLQALTETSAAEIQNPVAFLYRIGANLILDGVRQGKRTAARDQ
                     AWTETVGEVVDGVSIAHQPAQDDAVWARQKLDQVSRALEGVAPNARQAFRLHRIDGLT
                     HAQIAQRLGVSSSSVEKYLSGVLARLLREVGWP"
     gene            501..1478
                     /locus_tag="VF633_05115"
     CDS             501..1478
                     /locus_tag="VF633_05115"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX8470471.1"
                     /translation="MTLGLDHTESVIDQASAWVVRLGGDEVSDSDYEALGAWLDLSPS
                     HRAAFEEAEGLWAELGGDRAALDAALTRSAPRTSVRGTRRPDVSRRWIWGAGGLAAAA
                     LACAVLIGPALLSPTQTYVAAPGEQRTISLSDGSTIAMNGGSTLSVRMSGQERRVEMA
                     DAEAAFDVAHDADRPFRVTVGESRIEVLGTAFDVRRDGDTTKVNVTRGVVRVSDLQDA
                     GHNVRLTIGQSVTRTDAIGELRVAPGSTVPAGWRAGQLIYNDRPLSEVVEDLNRAYPM
                     PIRAVNGADRLRFTGVLALDGQDETLRRLQLFLPIAVDRRDAIIELRPR"
     gene            1490..>2485
                     /locus_tag="VF633_05120"
     CDS             1490..>2485
                     /locus_tag="VF633_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8470472.1"
                     /translation="MTWTFRHRLLGASFAWLLGATGAVGQEARRLSIPAQPMTTAVLQ
                     LGAQTGVSISALAAAGCGRSRAVEGRFRVEAALQVLLAGSGCTYRRLDRTTYLIVARE
                     TPPSPPPPRRPAPAPPLPVVPSELDDVIVTATRRELHLAGAPYALSSVDGVSFDAAAR
                     RDTSALATRLAGLTVTNLGPGRNKLFVRGLADSPLTGQTQAMGGLYLDETRLTYDAPD
                     PDLRLVDLARVELLRGPQSTLYGSGTLGGVLKLISRPPVLDEYQGEIAAGLTFADDTS
                     PGHSMDLVFNAPLLRDRLAVRTVLYEEVLEGVVDDPGQGRSNIGRTVRKGARSSLL"
BASE COUNT          373 a          868 c          810 g          434 t
ORIGIN      
        1 tgtccgcctt caggccctga ccgagacctc ggccgcagaa atccagaacc cggtggcgtt
       61 cctttaccgg attggcgcca acctgatcct cgacggagtc cgacagggaa agagaacggc
      121 ggcgcgagat caggcctgga ccgaaaccgt tggcgaggtc gtcgatggtg tttcgatcgc
      181 ccatcaacct gctcaggacg acgccgtgtg ggcccgtcaa aagctcgatc aggtcagccg
      241 ggcgctggaa ggcgtcgcgc caaacgcccg tcaggccttt cgcctgcacc ggatcgacgg
      301 cctgacccac gcccagatcg cgcagcgcct cggtgtctcc agcagctcgg tggagaaata
      361 cctgagcggt gtgctcgctc gtctgctgag ggaggtcggc tggccatgaa gacgagattt
      421 cgccattatt ggttgggggt gtccgggatc ggcggcgtct cttcatctgg acggcggccc
      481 ttccgctgga tcgaaaccgc atgacccttg gcctcgacca tactgagtcc gtcatcgacc
      541 aggcctcggc ctgggtcgtc cggctgggcg gcgacgaggt ctctgacagc gactacgagg
      601 ctttgggtgc ttggctcgac ctgtcgccct ctcatcgcgc cgccttcgag gaggcagaag
      661 gcctatgggc ggagctcggc ggagatcgcg ccgcactcga cgccgcactc acccgctcag
      721 cgcctcgcac ctccgtccgt gggacccgcc gaccagatgt gagcaggcgc tggatatggg
      781 gcgccggcgg gctcgccgct gccgcgttag cctgcgctgt gttgattggc cctgcccttc
      841 tctcgccgac ccagacctac gtcgcggccc cgggcgaaca gcgaacgatc agcctttccg
      901 acggctccac catcgccatg aacggcggct cgaccctgtc tgtacggatg tcgggccagg
      961 aacgaagagt tgagatggcg gacgcggagg ccgccttcga cgtggcgcac gatgccgatc
     1021 gcccgttccg cgtcacggtc ggcgaaagcc ggatcgaggt cctcggaacc gctttcgatg
     1081 tccgccgcga cggcgacacc accaaggtca acgtcacccg cggcgtggtc cgggtctccg
     1141 atctgcaaga cgccgggcac aatgtgcgcc tgacgattgg ccagtcagtg acgcggaccg
     1201 acgctattgg cgaacttcgc gtcgctcccg gatcgaccgt tccggcaggc tggcgcgccg
     1261 gtcagttgat ctacaatgac cgtcctttgt ccgaggtcgt ggaagacctg aaccgggcct
     1321 atccaatgcc gatccgcgcg gtgaacggcg cagaccgact acgtttcact ggcgtcctcg
     1381 ccctggacgg gcaggatgag accctgcgca ggctccaatt gttcctgccg atcgcggtcg
     1441 atcggcgcga cgccatcatt gagttgcgac cgcgctgacg cctgtctgca tgacctggac
     1501 tttccgccat cgcctgcttg gagcgtcgtt cgcgtggctg ctcggcgcca ccggagccgt
     1561 gggtcaggag gcccgccgcc tttcgatccc ggcccagccg atgaccacgg ctgtccttca
     1621 gctcggcgcc cagacgggag tttccatcag cgccctcgcc gccgccggct gcggccggtc
     1681 ccgtgcggta gagggaaggt ttcgcgtgga ggcggcgctg caagtgctgc tggccggcag
     1741 cggctgcacc taccgtcgtc tcgaccggac aacctacctg atcgtcgcgc gcgagacgcc
     1801 gccctccccg cccccgccgc gtcgcccggc gccagcgccg ccgctgcccg tcgtcccgag
     1861 cgagctggac gatgtcatcg tcactgcgac ccgccgtgag ctgcatttgg caggcgctcc
     1921 ctacgccctg tcctccgtcg acggcgtgtc cttcgatgcc gccgcccgac gcgacacctc
     1981 ggcgctggcg acgcggctgg cggggctgac cgtcaccaat ctggggccgg gtcgaaacaa
     2041 attgttcgtc cgaggcctcg ccgacagtcc cctgacaggc cagacccagg ccatgggcgg
     2101 gctctatctg gacgagacgc ggctgaccta cgacgccccc gatcccgatc tgcgccttgt
     2161 cgatctggcg cgggtagaac tgctgcgggg tccacagtcg accttgtacg gctctggcac
     2221 gctgggcggc gtgttgaagc tgatcagccg tccgcccgta ctcgacgaat atcaggggga
     2281 gatcgccgcc ggcctcacct tcgcggacga tacctcgccg ggccactcaa tggatttggt
     2341 cttcaacgcc cccctcctgc gcgaccggct ggcggtgcgt accgttctct acgaggaggt
     2401 gctggagggc gtcgtcgacg atcccggcca ggggcgatcg aatatcggcc gtaccgtccg
     2461 caagggcgct cgttcctcgc tgctc
//
LOCUS       DASVTM010000186         7461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_867348,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000186 DASVTM010000000
VERSION     DASVTM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867348"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1306
                     /gene="lptG"
                     /locus_tag="VF633_05125"
     CDS             167..1306
                     /gene="lptG"
                     /locus_tag="VF633_05125"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX8470473.1"
                     /translation="MRISLRGGHTGVRLRLGRIERYVLVQQMRALGVALAVISALVML
                     IDFVEISRGLGSDIDLSPLRIVGLMMIKSPQVIVQLLPFVFLFGTLSAFVSLNRRSEL
                     IAMRAAGISAWRFVLPAAGAAVVLGILTVALIGPLAAMGDNVFQRERARISGSVLGAD
                     EQSQAIWLREGEADRQMVIRAGRQDRNNARLLDVTFFIYTTDPNGRRAFTERIDAPSA
                     SLSAGRWRLINAIGAQTGQRAVRYATLDLPSSLADDEAFDRFARPQATSFWSLPAQIS
                     RIEAAGFSSTGYRLRLQQLLATPLMFAAMSILAAAFSLRLMRLGDLAKMAVGAVVLGF
                     AFFFLNQASSAFGSAEVVPPFVAAWLPAILTALAALTLLFYTEDG"
     gene            1351..3831
                     /gene="lptD"
                     /locus_tag="VF633_05130"
     CDS             1351..3831
                     /gene="lptD"
                     /locus_tag="VF633_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX8470474.1"
                     /translation="MQCDRGASNGTGLRTRLLAGAALIACAPWLLASQASAQAAPTAP
                     QTASAPAASQASEATGPDGLPPKAVYISAETVTQAGDVVTANGTTERVLARFQAHTLR
                     AGVVTYDLDKGVGSADRDVELTSPNGDTVYASHIELDSDLKAGVAVDFATRLSNGTSL
                     MAATAVRRSARVNELNYALFTPCTICDAEGNAKTPSIAIQAEKVVQDEELRAILFRNA
                     IFRVGGVPVFWLPVFAVPDPTVDRASGVLVPIINYDEGRGVSVEVPYLYVVSPSEDWI
                     FSPQFNTRVAPFLNLQWRRRFADGTIVARGGYTYAQSFGDFDLDGDGSAESNVDFGDR
                     TSRSYILAHGKFDPMGPWRWGFTAERTSDKTLFDRYDVRDPYQDNGLYYGDQRRLISQ
                     LYAERQTERSYLSIAAFSIQSLRVAEFNTITPALNVFEDDKTLPLVAPLIDARWEPAE
                     PILGGRLRLKASAVSLSRNAYVGSPVLRPEIIPAGSTDGFGGVDSRRTTLQGEWRRAF
                     ITAAGVRYEPFLDARFDAYSISDLPPMLGRDSDVITRGRATAGVDVSYPMIKRLSATS
                     DLIIEPMAQLSISNRPDLDPRIPIEDTQTFELDESSLFRTDRLPGYDFYEGGARFTAG
                     ARATLRWDDIRKASLFVGRSIRSDEEDAFRTSIPDAPASLYDPSGLASQTSDWVVQAS
                     FSPTDRIRGWGHATVDGSGDIRRAEAAIDGTWGRRNMASVSYIVDRSNPLAGPNNRNY
                     EFVQLQGQQFVYGNWGVAVTGIADLERNLITRSEVGLLFDDDCFRFELGFRQDNTRVR
                     PSGPSEGVYVRLNLATFGGTGYGRNGNR"
     gene            3917..5296
                     /locus_tag="VF633_05135"
     CDS             3917..5296
                     /locus_tag="VF633_05135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8470475.1"
                     /translation="MRYTTGVAIAALLAGSAMAQTAPIPAAAQTPAARPPASQTAASQ
                     AAGALNPAAEQAPVRPAAAPPQFKISEGVIASVNDQVITSFDLRQRMLMIIAMSQVQP
                     TEENIPAIQQQALNALVEERLQTQELAKYKDLVISDAEVDEEIASMAQEAGATPANYM
                     SFLEQGGIRPQNVREQLRTEIGWRALVGGRFNSRAKVSRAAVDQAMRQLTETASKPQY
                     LIGEIYIEAARVGGQQAAMTGAEQLVQQMVQGAPFQNVARQFSAAPSASRGGDAGWVV
                     QGTIQPPLQAALDQLQVGQLSRPIPVEGGVYIVYMRDKRSGSATNLVSMKQIMVELPE
                     TASDADVAAATARLEAVRPQLTCDNILQRATSEQGLLGSDLGESDIANLAPQFQQVAR
                     AAAIGTVSSPVRTPLGVHLLAVCGRRAGGPDAPTYQQVENRLQNQNLAMLARRYIRDL
                     RSDALIEMK"
     gene            5301..6302
                     /gene="pdxA"
                     /locus_tag="VF633_05140"
     CDS             5301..6302
                     /gene="pdxA"
                     /locus_tag="VF633_05140"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX8470476.1"
                     /translation="MAAPRPLVLSMGEPAGIGPEIIAKAWRALSADGLPFVVIGDAAL
                     MEAQGVPVADIGQVADASIRFTRALPVLNHPAPAPVVIGTPDPANAGVVADWIERGVD
                     LILSGEASGLVTAPIAKAPLYAAGFRFPGHTEFIAELTADAPFSGTRGPVMMLTAQNL
                     RACLVTIHVALDQVPELVTVERVCRTARVVHESMKRDFGIDRPRLALAALNPHAGEGG
                     ALGLQEITVLRPAVEILRAEGITITDPLPADSMFHEKARPTYDAAICLYHDQALIPVK
                     TLDFWGGVNATLGLPIIRTSPDHGTGFDIAGKGIARADSLIAAIRLATEMAAARASR"
     gene            6311..7132
                     /gene="rsmA"
                     /locus_tag="VF633_05145"
     CDS             6311..7132
                     /gene="rsmA"
                     /locus_tag="VF633_05145"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX8470477.1"
                     /translation="MAAELPPLRESLEAHGLLAKKSFGQHFLLDLNVTRKIVRLAGPF
                     EGRAVIEVGPGPGGLTRAILESDAGKVVLVEKDPRFVPLLEELDKGDGRLTIVQADAL
                     KVDEAALTEGPTHLVANLPYNVGTPLLIKWLTGPWTPCALTLMFQKEVAERIVAATDD
                     DAYGRLAVITQAVAEAKIVMHLPPAAFTPPPKVSSAVVHLVPRADRPDRATLKRLETV
                     TAAAFGQRRKMLRSSLKQLGGGALCDAAGIDPDARAETIDVAGFLRLANALETKA"
     gene            7129..>7461
                     /locus_tag="VF633_05150"
     CDS             7129..>7461
                     /locus_tag="VF633_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX8470478.1"
                     /translation="MMSDIEPFRAITISRLAHALKAEGRSVIHMEFGQPSTGAPAAAI
                     AEAHRVLDVDGMGYWESTPLKARIAKLYQDRYGVDVDPDRLLLTCGASPALVLALSSR
                     FKPGDRIAM"
BASE COUNT         1234 a         2624 c         2306 g         1297 t
ORIGIN      
        1 gctatggcgt cgtcgccgcg agcgcgtgga acggctggct gaacgttttc cagtacctgc
       61 tgccgctagt ggctgctgcg gtggcgctga gattgctgtt ccgggcgctc aagcctcgca
      121 agggccggac gataccggcc gttgagcgct tcaaggcgag gttcgcatga ggatttccct
      181 gcgcggcggc cacaccggcg tccggctccg gctgggccgg atcgagcgct atgtgctggt
      241 tcagcagatg cgcgcgctgg gtgtcgccct cgccgtcatc agtgcgctgg tgatgctgat
      301 cgatttcgtc gaaatttcgc gcggactggg ctccgacatc gacctctctc cgctgcgcat
      361 cgtcggcctg atgatgatca agtccccgca ggtgatcgtc cagcttctgc cgttcgtctt
      421 cctgttcggg acactgtcgg ccttcgtcag cctgaatcgg cgcagcgaac tgatcgccat
      481 gcgcgccgcg ggcatctcgg catggcgatt cgtcctgcct gcggcgggcg cagctgtcgt
      541 gcttggcatc ctgactgtcg ccctgatcgg tccgttggcg gcaatgggcg ataacgtctt
      601 ccagcgcgag cgcgcccgta tctccggttc agtccttggc gcagacgaac aaagtcaggc
      661 gatctggctg cgtgaaggcg aagccgaccg tcagatggtc atccgcgccg gtcgtcagga
      721 ccgcaacaac gcccggctgc tggacgtgac cttcttcatc tacaccaccg acccgaatgg
      781 tcgccgcgcc ttcaccgaac ggatcgacgc cccttcggcc agcctcagcg ccgggcgctg
      841 gcggctgatc aacgcgatcg gcgcccagac cggccagcgc gccgtgcgct acgccaccct
      901 cgacctgccc tccagcctgg cggacgacga agcctttgat cgcttcgcca gacctcaggc
      961 aacctcattc tggtccctgc ccgcccagat cagtcgcatc gaggccgctg gattttcctc
     1021 gaccgggtac cgactgcggt tgcagcagct tctggccacg ccgctgatgt tcgcggccat
     1081 gtcgatcctc gccgccgcct tctcgctcag gctgatgcgg ctgggtgatt tggccaagat
     1141 ggcggtgggc gccgtggtgc tcggcttcgc cttcttcttc ctcaatcagg cctcatccgc
     1201 cttcggttcg gcggaggtcg tgccgccgtt cgtagcggcc tggcttccgg cgatactgac
     1261 ggcgctcgcc gccttaacct tgctgttcta taccgaagac ggctaagcga ccgatcggcg
     1321 ggggccgaag aaaaacggac ttgagtttgt atgcaatgtg atcgcggcgc ctcgaatggc
     1381 acaggcctgc ggacccggct tctggctggc gccgccctga tcgcctgcgc gccgtggctg
     1441 ctggccagcc aggcgtcggc gcaagccgca ccgaccgctc cccagaccgc ttcagcgcct
     1501 gccgcatccc aggcctccga agctacgggt ccggatgggc tgccgcccaa ggccgtctat
     1561 atcagtgccg agaccgtcac ccaggcgggg gacgtcgtca ccgccaacgg gacaaccgaa
     1621 cgcgttctgg cccgcttcca ggcccacacc ctgcgtgcgg gtgtagtcac ctacgatctg
     1681 gacaagggcg tcggcagcgc ggatcgcgat gtcgagctga ccagccccaa cggcgacacg
     1741 gtctacgcga gccatatcga actggactcc gaccttaagg ctggcgtcgc cgtcgatttc
     1801 gctacgcggc tgtcgaacgg caccagcctg atggcggcca cggcagtgcg ccggtctgcc
     1861 cgggtcaacg aactgaacta cgccctgttc accccctgca ccatctgcga cgccgagggc
     1921 aacgccaaga ctcccagcat cgccatccag gcggaaaagg tggttcagga tgaagaactc
     1981 cgggcgatcc tattccgcaa cgccatcttc cgcgtcggcg gcgtgccggt gttctggctg
     2041 cccgttttcg ctgtgccgga cccgaccgtt gatcgtgcct cgggcgtgct ggtcccgatc
     2101 atcaattatg acgagggccg gggggtctcc gttgaggtcc catacctgta cgtcgtctcg
     2161 ccctcagagg actggatttt cagcccacag ttcaacacgc gggtggcgcc gttcctgaac
     2221 ctgcaatggc gtcgccgttt cgcggacggt acgatcgtgg cgcggggtgg ctatacctat
     2281 gcccagtcgt tcggtgactt cgacctcgac ggcgacggca gcgccgaaag caacgtcgat
     2341 ttcggcgacc gcacaagccg cagctatatc ctcgctcacg gcaaattcga tccgatgggg
     2401 ccttggcgct ggggcttcac agctgagcgg acctcggaca agacactgtt cgaccgctat
     2461 gacgtccgcg atccctatca ggacaacggc ctatattacg gcgaccagcg ccggttgatc
     2521 agccagctct acgccgaacg ccagaccgaa agatcctacc tctcgatcgc cgccttctcg
     2581 atacaaagcc tgcgggtcgc cgaattcaac accattacgc cggcgttgaa cgtcttcgag
     2641 gacgacaaga ccctgccgct ggtcgcgccg ctgatcgatg cacgctggga gcccgcggag
     2701 ccgatcctcg gaggacgttt gcggctcaaa gcgtcagccg tatcgctctc gcggaacgcc
     2761 tatgtcggca gcccggtgct gcggcccgaa ataattcctg ccggttcgac cgatggcttc
     2821 ggcggcgtgg acagccgtcg cacgacgctg cagggcgaat ggcgtcgggc ttttatcact
     2881 gcggcgggcg tccgctacga gccgttcctc gacgcgcgtt tcgacgccta ttccatctcg
     2941 gacctgccac ctatgttggg acgggacagc gacgtcatca cccggggacg cgccactgcc
     3001 ggcgtcgacg tcagctatcc gatgatcaaa cgcctcagcg ctacgtcgga tctgatcatt
     3061 gagcccatgg cccagctctc catttccaac cggcccgatc tcgacccgcg cattcccatc
     3121 gaagacaccc agaccttcga gctcgacgaa agctcgctgt tccgcacgga ccgactcccc
     3181 ggctacgact tctacgaagg cggcgcgcgg ttcaccgccg gcgcgcgcgc gaccctgcgc
     3241 tgggacgaca tccgcaaggc cagcctgttc gtcggtcgca gcattcgatc cgacgaggaa
     3301 gacgccttcc gaacctccat ccctgacgcc cctgccagcc tctacgatcc gagcggcctc
     3361 gcgtcccaga cctccgactg ggtggttcag gccagcttct cgcccaccga ccgcatccgc
     3421 ggctggggcc acgcgacagt tgatggctcg ggcgacatcc gccgagccga ggcggccatc
     3481 gacggcactt ggggccggcg caacatggcc agcgtgagct atatcgtcga tcggtcgaat
     3541 cccctcgccg gccccaacaa ccggaattac gagttcgtgc agcttcaggg gcagcagttc
     3601 gtttatggca actggggcgt ggcggtcaca ggcatcgccg atcttgagcg caacctgatc
     3661 acccgatccg aggtcgggct gctgttcgat gacgattgct tccggttcga actgggtttt
     3721 cggcaggaca acacccgcgt gcggccctcc ggcccgtccg agggcgtcta cgtacgccta
     3781 aacctcgcca cttttggcgg tacaggctac ggacgaaacg gcaaccgatg aacacatcgc
     3841 ttgcaggggc gcgacctccc cgcacaatgg ggtcgagagt gcgcttcttg aggatccagg
     3901 actgactatg cctttgatgc gttatacgac tggcgtcgcg atcgccgcgc ttctcgcagg
     3961 ctcggccatg gcccagaccg ccccgattcc ggctgctgcc cagacccctg cggcccgccc
     4021 cccggcgtcc cagacggccg cgtctcaggc cgctggcgcg ttgaaccccg ccgctgaaca
     4081 ggcgcccgtt cgcccagccg cagccccgcc ccagttcaag atttccgaag gcgtcatcgc
     4141 ctcggtcaac gatcaggtca tcaccagttt cgatctacga cagcgcatgc tgatgatcat
     4201 cgccatgagc caggtgcaac cgaccgagga gaatatcccc gcgatccagc agcaggccct
     4261 gaacgccttg gtcgaagagc ggctgcagac gcaggaactc gccaagtaca aggatctggt
     4321 gatcagcgac gccgaggtag acgaggaaat cgcctcgatg gcccaggagg ccggcgcgac
     4381 gcccgccaac tacatgagct tcctcgagca gggcggcatt cgtccgcaga atgtgcgcga
     4441 gcagctccgc accgaaatag gctggcgcgc cctcgtcggc ggtcgcttca acagccgggc
     4501 caaggtttct cgcgccgccg tcgatcaggc aatgcgtcag ctgaccgaga ccgcctccaa
     4561 gccgcagtat ctgattggcg aaatctacat cgaggccgct cgtgtcggtg gccagcaggc
     4621 ggcaatgacc ggcgccgagc agctggtcca gcagatggtt cagggcgcac cgttccagaa
     4681 cgtggcccgt cagttttcgg cggctccatc ggcctcgcgc ggcggcgacg ccggctgggt
     4741 cgttcagggc accatccagc cccccctgca agcggcgctg gaccagcttc aggtcggcca
     4801 gctctcgcga cccattccgg tcgagggcgg cgtctacatc gtctacatgc gcgacaagcg
     4861 gtccggctct gccaccaacc tggtgtcgat gaagcagatc atggtcgagc ttcctgaaac
     4921 ggcttccgac gctgacgttg cagccgcgac ggcccggctt gaggccgtcc gcccgcagct
     4981 gacctgcgac aacatcctgc agcgcgcaac gtcggaacag ggcctgctcg gctccgacct
     5041 gggcgagtca gacatcgcca acctcgcgcc ccagttccag caggtcgccc gcgcggctgc
     5101 gattggcacc gtcagttcgc cggttcgtac gccgctgggt gttcacctgc tcgccgtctg
     5161 cggtcgtcgc gccggcggtc cggacgcccc gacttatcag caggtcgaaa accgcctgca
     5221 gaaccagaac ctcgccatgc tcgcccgccg ctacatccgc gatctgcggt cggacgccct
     5281 gatcgagatg aagtaagcgg atggccgcgc cccggccact ggtgctatcg atgggcgagc
     5341 ctgcgggcat cggcccggag atcatcgcca aggcctggcg cgcgctgtcc gccgatggtt
     5401 tgccgtttgt cgtcatcggc gacgcggctc tgatggaagc ccagggcgta cccgtcgctg
     5461 atatcggtca ggtcgctgac gcctccatcc ggttcacccg cgcgctcccg gtgctcaacc
     5521 atcccgcccc tgcgccagtc gtcatcggta cgcccgatcc ggccaacgcg ggcgtcgtgg
     5581 ccgactggat cgagcgcggc gtcgatctca tcctgtcggg cgaagcgtca gggctcgtca
     5641 ccgccccgat cgccaaggcc cccttgtatg ccgccggctt ccgctttccc ggccacaccg
     5701 agttcatcgc cgaactgacc gccgacgcgc ctttctcggg cacgcgcggg ccggtgatga
     5761 tgctcacagc ccaaaatctg cgcgcctgtc tcgtcaccat ccatgtcgcg ctcgatcagg
     5821 tgccggaact cgtcacggtc gagcgcgtct gccgcaccgc gcgcgtcgtg catgaatcga
     5881 tgaagcgcga cttcggcatc gaccgtcccc gtctggccct ggccgcactg aacccgcacg
     5941 ctggcgaagg cggggcgctg ggattgcagg aaatcaccgt gctgcgcccc gcggtcgaga
     6001 tcctgcgcgc cgaggggatc acgatcacag acccgctgcc tgccgattcg atgttccacg
     6061 agaaggcccg cccgacctat gacgcggcca tctgcctgta ccacgatcag gcgctgatcc
     6121 ccgtcaagac gctggacttc tggggcggcg tcaacgccac cctcggcctg ccgatcatcc
     6181 gcacctcacc cgaccacggc accggcttcg acatcgcagg caaaggcatc gcccgcgcag
     6241 atagcctgat cgccgccatc cgtttggcga cggagatggc ggcggcgcgc gccagccgct
     6301 agacggacga atggctgccg aactcccccc tctccgcgaa tccctcgaag cccacgggct
     6361 gttggccaag aagagtttcg gccagcattt cctgctggac ctgaacgtca cccgcaagat
     6421 cgtccgcctg gcagggccgt tcgaaggccg cgccgtgatc gaggtcggac ccggccccgg
     6481 cggcttgacg cgcgccatcc tcgaaagtga cgccggcaag gtggtgttgg tcgaaaagga
     6541 cccgcgtttc gtccccctgc ttgaagaact ggacaagggc gatggccgcc tgaccatcgt
     6601 tcaggccgat gcgctcaagg ttgatgaggc ggcactgaca gagggaccga cgcacctggt
     6661 cgcgaacctg ccctacaacg tcggcacgcc tctgctgatc aaatggctga ccggtccgtg
     6721 gacaccgtgc gccctgaccc tgatgttcca gaaggaagtg gccgagcgga tcgtcgccgc
     6781 gacggacgac gatgcctacg gtcgcctcgc cgtgatcacc caggcggtgg ccgaggcgaa
     6841 gatcgtcatg cacctgccgc ccgccgcctt caccccgccc ccgaaggtgt cgtcggcggt
     6901 cgtacatctc gtaccccgtg ccgatcgccc cgaccgcgcg acgctgaaac ggcttgaaac
     6961 cgtgaccgcc gccgccttcg gccagcgccg caagatgctg cgctccagtc tgaagcaact
     7021 tggtggaggc gccctgtgcg acgcggcagg catcgacccc gacgcccgcg ccgaaaccat
     7081 cgacgtcgcg ggcttcctga ggctggcgaa cgcgctggag accaaagcct gatgtcggac
     7141 atcgaaccgt tccgcgccat caccatcagc cgtctggcgc acgcgctcaa ggccgagggc
     7201 cgtagcgtaa tccatatgga attcggccag ccttcgacgg gagccccggc cgccgccatc
     7261 gcagaagcgc accgggtgct ggacgtggac ggcatgggct attgggagag cacgccgctc
     7321 aaggcgcgta tcgccaagct gtatcaggat cgctacggcg tcgatgtcga cccggaccgg
     7381 ctgctgctaa cctgcggagc ctcgccagcg ctggtgctgg ccctgtcttc gcgcttcaag
     7441 cccggtgacc ggatcgccat g
//
LOCUS       DASVTM010000187         4441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_655744,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000187 DASVTM010000000
VERSION     DASVTM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655744"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF633_05155"
     CDS             <1..208
                     /locus_tag="VF633_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX8470479.1"
                     /translation="DGTRVKVLMSRAEPTHTGAPGQILDEDLLVACGAGAVRLIRVQR
                     EGKAVQTAADLLKGFPLPAGTLLG"
     gene            208..948
                     /gene="truA"
                     /locus_tag="VF633_05160"
     CDS             208..948
                     /gene="truA"
                     /locus_tag="VF633_05160"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX8470480.1"
                     /translation="MPRYRLTIEYDGANYAGFQAQAGLPTVQGAIETAVKGFTGQTVR
                     IAAAGRTDSGVHATGQVVHVDLEKDWPAETVMNALNAHLVKEGVAVLDSTIVGDDWHA
                     RFSATGRRYLFRILNRPGRPALERGKVWHMRKPLDATAMHEAAQALVGHHDFTTFRDL
                     ACQSKSPEKTLDVARISRVGEEVHLVFEARSFLHRQVRSMTGTLVEVGLGRWTAQDVA
                     EALAARDRTACGPVAPSDGLYLTGVSYE"
     gene            complement(1023..1160)
                     /locus_tag="VF633_05165"
     CDS             complement(1023..1160)
                     /locus_tag="VF633_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin EcnA/B family protein"
                     /protein_id="HEX8470481.1"
                     /translation="MRKIITLSIIAAALAVSACNTIAGAGRDVSTAGSVVTGAANDAK
                     N"
     gene            1264..2934
                     /locus_tag="VF633_05170"
     CDS             1264..2934
                     /locus_tag="VF633_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8470482.1"
                     /translation="MSEMIPNTARRLVDTLVMNGVERVFCVPGESYLAVLDALADVTD
                     QIEVITCRHEAGAANMAEAYGKLTGKPGICMVTRGPGATHASIGVHTAHQDSTPMILF
                     VGQIAISDRGRGAFQEVDYREVFGGLAKWATELETGTRTVEVVERAFATALQGRMGPV
                     VIALPEDVLEADGGPAPIRPVTPARSALDPAFVAEVGSHLAKAERPVLVLGGSGWTQE
                     ATTAIGDWAERIGLPLALSFRRKDLISNDRPNYVGDLGLGPNPALVQRVKDADLIIAI
                     GARLGENPTQGYTLLDRTKTPQSLIHIHPGPEELGRVWPPLMSAAADVSLAAIALSEI
                     EPGRTWHDQANTGHAAYQAFTAPVAVTGAANMSECMAHLRTVLPADAILTNGAGNYAA
                     WLHRFHKHTAMHTQLGPTSGAMGYGYPAAVAAKSLYPDRDVVCIAGDGEYLMTGQELA
                     TAVQYGINTVTVIVDNGTYGTIRMHQERDYPRRVIATDLRNPDFVQYARAFGAFGIRC
                     ETTGDFPAAFAAAREAASGADGVPAIVHLITSAEDIAPGRTITALRGE"
     gene            2945..3406
                     /locus_tag="VF633_05175"
     CDS             2945..3406
                     /locus_tag="VF633_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470483.1"
                     /translation="MMRLAACLTLIATVVTSACTTDPAGPGGPLRSADNGNDCAVIAA
                     VAREHYKFNTTDRLPPPLWLKDGATAWNPRCDWGRYDLSFPATYVPRVNRHGPEPMQW
                     VSFEKPVYDGRGATITSGILHGPLAGMGVECRVVSGIAGWTVSECRNSWIS"
     gene            3465..3809
                     /locus_tag="VF633_05180"
     CDS             3465..3809
                     /locus_tag="VF633_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX8470484.1"
                     /translation="MNAAARKALIAEYKDRPTVAGVYAVICTATGQVWVGQSRHIDTR
                     QNGLWFTLKTGGGRDPSLQTAWSAHSPEDFRFEQLDRLPTDLSEMRRKDELRERTARW
                     VQRLQAETLLPG"
     gene            3859..>4441
                     /locus_tag="VF633_05185"
     CDS             3859..>4441
                     /locus_tag="VF633_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX8470485.1"
                     /translation="MTPRPPFTATVLTMFPEAFPGPLGVSLIGTAWREKGLWSLQTLD
                     IRDFSEDKRGFLDDTPAGGGPGQVLKADVVARALDSVAASSRPLLYMSARGRPQTQAR
                     VKEWVFPLQGGVADGITVLCGRFEGVDQRVLDARGFEEVSVGDAVLAGGEAAAMVAIE
                     ACVRLVPGVLGNDASAGDDSFEDGLLEHPQFTRP"
BASE COUNT          768 a         1464 c         1464 g          745 t
ORIGIN      
        1 cgacggaaca cgcgtgaaag tgctcatgtc gcgcgccgag ccgacccaca ccggagcacc
       61 gggtcaaatc ctcgacgagg acttgctagt cgcgtgcggt gcgggggccg ttcgtctgat
      121 ccgcgtccag cgtgagggca aggccgtgca aactgcagcc gatctgctca aagggttccc
      181 cctgcccgcc ggaaccctgt tgggctgatg ccccgctacc ggctgaccat cgagtacgac
      241 ggcgcgaact acgcaggctt tcaggcgcag gcgggactac cgaccgtgca gggtgcgatc
      301 gagacagcgg tgaaaggctt taccggccag accgtgcgga tcgccgccgc tggacgcacc
      361 gattccggcg tccacgccac cggtcaggtc gtccacgtcg atctggaaaa ggactggccc
      421 gccgagacgg tgatgaacgc tctcaacgcc catctggtga aggaaggcgt cgccgtcctc
      481 gacagcacca tcgtgggcga cgactggcat gcgcgcttct cggcgaccgg gcggcgctat
      541 ctgttccgca tcctcaaccg cccgggccgt ccggctctgg agcgtggcaa ggtctggcat
      601 atgcgcaaac cgcttgatgc cacggccatg cacgaggcgg cgcaggcgct ggtcggccat
      661 cacgacttca ccaccttccg cgacctcgcc tgccagtcga aatcgccaga gaagacgctc
      721 gacgtcgccc ggatcagtcg cgtcggcgaa gaggtccatc tggtgttcga ggcccgctcc
      781 ttcctgcatc gtcaggttcg gtcgatgacc gggaccctcg tcgaggtcgg tctcggccgc
      841 tggaccgcgc aggacgtggc cgaggcgctg gccgctcgcg accgcaccgc ctgcggtccg
      901 gttgcgccct cggacggcct gtatctgacc ggcgtcagct acgaataacg ccctccacag
      961 accgcaaaca caaaaggccc gccggatccc ggcgggcctt tcaaacatcg ggcgcggaag
     1021 ccttagttct tggcgtcgtt cgcggcgccc gtgacgaccg aaccggcggt cgagacgtcg
     1081 cggccagcgc cggcgatggt gttgcaggcc gaaacggcga gagcggcggc gatgatggac
     1141 agggtgatga tcttgcgcat gagagattct ccgggggttg tgcgtttaac ctgagccatg
     1201 aacgtcaagc gtgaccattg ggttgcatgg tgatttcgca gttcgatctg ctaaatcaga
     1261 gccatgagcg aaatgatccc gaacaccgcc cgccgtctcg tcgacaccct ggtgatgaac
     1321 ggggtggagc gagtcttctg cgtcccgggc gagagctatc tcgccgttct cgacgccctc
     1381 gccgacgtga cggatcagat cgaggtcatc acctgtcggc atgaggcagg cgccgccaac
     1441 atggctgagg cctatggcaa actgaccggc aagcccggca tctgcatggt cacgcgaggg
     1501 cctggcgcga cccacgcctc gatcggcgtc catacggcgc atcaggactc cacgccgatg
     1561 atcctgttcg tcggtcagat cgcgatttca gaccgtgggc ggggcgcctt tcaggaagtc
     1621 gattaccgcg aggtcttcgg cggtctggcc aaatgggcga ccgaactgga gaccgggacc
     1681 cgcacggtcg aagtggtcga gcgggccttc gccaccgcgc tgcagggtcg gatggggccg
     1741 gtggtcatcg ccctgcccga ggatgtgctg gaggccgacg gcggcccggc gccgatccgt
     1801 cctgtgacgc ctgcccgatc cgcgctggat ccggcgttcg tcgctgaggt cggatcgcat
     1861 ctggccaagg ccgaacgtcc cgtgctggtt ctcggcggct ccggctggac acaggaagcg
     1921 accaccgcca tcggcgactg ggccgagcgg atcggcctgc cgttggccct gtcgttccgg
     1981 cgcaaggatc tgatctccaa cgaccggccc aactatgtcg gtgatctggg gctcgggccg
     2041 aacccggcgc tggtccagcg ggtcaaggat gccgacctga tcatcgccat cggcgcccgg
     2101 ctgggtgaaa atccgacgca gggctacacc ctgctcgacc ggacgaagac gccgcaatcg
     2161 ttgatccata tccacccggg gccagaggaa ctcggtcgcg tctggccgcc gttgatgagc
     2221 gccgccgccg acgtgtcgct ggcggccatc gccctgtcgg agatcgaacc gggccggaca
     2281 tggcacgatc aggccaacac gggccatgcc gcctatcagg ccttcaccgc cccggtcgcg
     2341 gtcactggcg cggcgaacat gagcgaatgc atggcgcatc tgcgcaccgt cctacccgcc
     2401 gacgccatcc tgaccaacgg cgcgggaaac tatgcggcct ggctgcaccg cttccacaag
     2461 cacaccgcca tgcacaccca gctcggtccg acctcgggcg ccatgggcta cggctatccg
     2521 gcggcggtag cggccaagag cctgtatccc gaccgcgacg tggtctgcat tgccggggac
     2581 ggcgaatacc tgatgaccgg tcaggagctg gcgacggcgg tccaatacgg catcaacacg
     2641 gtcacagtga tcgtcgacaa cggcacctat ggcaccatcc gcatgcatca ggagcgcgac
     2701 tatccgcgcc gggtcatcgc caccgacctg agaaaccctg actttgttca atacgcccgg
     2761 gcgtttggag ccttcggcat tcgctgcgag accacgggag acttccccgc cgcattcgcg
     2821 gcagcgcggg aagcggcctc aggggccgac ggcgttccgg ctatcgtcca cctgatcacc
     2881 tcggccgagg atatcgctcc cggccggacc atcaccgccc tgcgcggcga atagacggag
     2941 cctgatgatg cgcctcgccg cctgcctcac gctgatcgca accgtcgtga cctccgcttg
     3001 caccacagat cctgccggtc caggcggtcc cttgcgttcg gcagacaacg gcaacgactg
     3061 cgcggtgatc gcggcggtgg ccagggagca ctacaagttc aacaccaccg accgtctgcc
     3121 gccgccgctg tggctcaagg acggggccac ggcatggaac ccccgctgcg actggggcag
     3181 atacgacctt agttttccgg cgacctatgt tccacgggtc aatcgccacg gccctgaacc
     3241 catgcagtgg gtctcgttcg agaagcctgt gtacgatggc cgcggcgcga caatcaccag
     3301 cggcatcctg cacggtcccc ttgccgggat gggagttgaa tgccgggtcg tctcgggcat
     3361 cgccggctgg actgtcagcg agtgccgcaa tagctggatt tcctaatcta tttacccggg
     3421 ttaaaattaa ggctattgtg cccgggtaaa acaggaagcc cgatatgaac gccgccgccc
     3481 gcaaagcctt gatcgccgag tacaaggatc gtccgaccgt tgctggcgta tacgccgtca
     3541 tctgtaccgc aacgggtcag gtctgggtcg gtcagagccg ccatatcgac acgcgtcaga
     3601 acggcctctg gtttacgctc aagacgggcg gcggtcgcga tccgtccctg cagacggcat
     3661 ggagcgccca ttcgcccgag gatttccgct tcgagcaact ggaccgcctg cccaccgatc
     3721 tctcggagat gcggcgtaag gatgaactgc gcgaacggac cgccagatgg gtgcagaggc
     3781 tgcaggccga aacgcttctg ccgggatagc cgaagcgcag gcgtggattt tcgtcccctt
     3841 caggcgctaa aggcggtcat gaccccacgt ccgcccttca ccgccactgt cctgaccatg
     3901 ttccccgagg cctttcccgg gccgctcggc gtctcgctga tcggcacggc ttggcgtgag
     3961 aaggggctgt ggagtctgca aacgctggac attcgggact tttccgaaga taagcgcggc
     4021 ttcctggacg acacccctgc gggtggcggc cccggacagg tactcaaagc ggacgtggtg
     4081 gcccgggcac tggacagtgt tgcggcgtcg tcccggccgc ttttgtacat gagcgcacgc
     4141 ggtcggcccc agacgcaggc gcgagtgaaa gagtgggtct ttcctctgca aggaggggtg
     4201 gcggacggta tcaccgtcct ctgcggccgg ttcgaggggg tggatcagcg ggtgctagac
     4261 gcacgcgggt tcgaggaagt ctccgtcgga gacgcggttc tcgccggtgg cgaggcggcg
     4321 gcgatggtgg ccatcgaggc gtgcgtgcgt ctggttccgg gggttctcgg caatgacgcg
     4381 agcgcgggag acgacagctt cgaggacggg cttctcgagc atccgcagtt cacgagaccg
     4441 c
//
LOCUS       DASVTM010000188         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_497878,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000188 DASVTM010000000
VERSION     DASVTM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497878"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VF633_05190"
     CDS             complement(<1..1031)
                     /locus_tag="VF633_05190"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8470486.1"
                     /translation="MNRHASLLATAAVALVLSTPAFAQQSSSATAPAATSTSNHAVIG
                     DWGFDPSSMDVTTKPGNDFNRYASGGWLDATTIPADRPSWSVWDVIYDQGQEQLKAII
                     ANSAAHPGSSPEAQRIGDFYASYMDEAAVNALGAKPLEPGLAAIRAAHDRTDIAKLMG
                     ESFGRAGNSLFTIYIDSDLKAPDTNSAYLGQSGLGLPDRDYYLEASFADKKTAYQAYV
                     AQMLGKIGWADADKAAADIVAFETKIAEKHWTRAQSREMDDTYNAMTLAEVAAYAPGF
                     DWAAWSTAAGIPATAHTVVGEKTAFPELARIFAETDLDTLKAYEAFHYVDQSASVLSS
                     DFVDARFAFR"
     gene            complement(1117..1713)
                     /gene="purN"
                     /locus_tag="VF633_05195"
     CDS             complement(1117..1713)
                     /gene="purN"
                     /locus_tag="VF633_05195"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX8470487.1"
                     /translation="MPTTTGDRTRVAVMISGAGSNMAALIDAGQTPDSPYQVVLVVSN
                     KPDAGGLHIAEAKGVRAVAVDHRDFPKDREAHERAIDALLAEADVQIIALAGYMRVLT
                     PYLVQRWEGRMINVHPSLLPLYPGLDTHARAIAAGDAEGGCTVHIVTDGVDEGPVLGQ
                     ARVPILRGDTPDALAERVHVAEHQLYPRVLTELCRSLH"
     gene            complement(1713..>2985)
                     /locus_tag="VF633_05200"
     CDS             complement(1713..>2985)
                     /locus_tag="VF633_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8470488.1"
                     /translation="QIQPERMRSDITALVGFGTRHTLSQTDSDTRGIGAARRWAESQL
                     RTISTECGGCIEIALPADTVTGRRVPTPTEVVNVLGIQKGTGDSKRVIVISGHIDSRV
                     TDVMDFTSDAPGANDDASGVAAVLEAARVLSKQKFDATLVYAVLSGEEQGLLGGKILA
                     DYAKAQGWVVEANLNNDIVGNSEGQSGVRDTTRIRVFSEGTKTVETHEQAATRRYNGG
                     ELDSSSRNLARFVDSIADRYLTNFDVEMIYRTDRFGRGGDQVEMLKAGFPAVRITEGA
                     ENYDRQHQDLRTENGTVYGDTIEGVDFAYLGQVARLNIVTMAAMAKAPTVPTEVAITG
                     AVLPDTTVKWSAVPGAAGYRVWWRATTAPQWTNSRWAGEATTLVLKDIPIDDYFFGVS
                     SVSADGYESPIVFPGPAGAFVSEAPPTPPAA"
BASE COUNT          467 a          879 c         1102 g          537 t
ORIGIN      
        1 cggaaggcga agcgggcgtc gacgaagtcg gacgacagga ccgacgccga ctgatctacg
       61 tagtggaagg cctcataggc tttcagcgtg tcgaggtctg tctcggcgaa gatgcgcgcc
      121 agttccggaa aggcggtttt ctcgccgacg acggtgtggg cggtggccgg aatgccggct
      181 gcagtggacc atgcggccca gtcgaagccg ggcgcatagg cggcgacctc ggccagcgtc
      241 atggcgttgt aggtgtcgtc catctcgcgc gactgggcgc gggtccagtg tttctcggcg
      301 atcttcgtct cgaaagcgac gatgtcggcg gcggccttgt cggcgtcagc ccagccgatc
      361 ttgccgagca tctgggcgac ataggcctga taggcggtct tcttgtcggc gaaggacgct
      421 tccagatagt agtcgcggtc gggcaggccg aggccggact ggcccagata ggcgctgttg
      481 gtgtcgggcg ccttgaggtc tgagtcgatg tagatcgtga acaggctgtt gcccgcccgg
      541 ccgaagctct cacccatcag tttggcgatg tcggtgcggt catgggcggc gcggatggcg
      601 gccagaccgg gttccagcgg cttggcgccg agcgcgttga ctgcggcttc gtccatgtag
      661 ctggcgtaga agtcgccgat gcgctgggct tcgggcgaag acccggggtg ggcggcgctg
      721 ttggcgatga tcgccttcag ttgctcctgg ccctgatcgt agatcacgtc ccagacgctc
      781 cacgacgggc ggtccgccgg gatggtggtg gcgtcgagcc agccgcccga ggcgtagcgg
      841 ttgaagtcat taccgggctt ggtcgtcaca tccatgctgg agggatcgaa gccccagtcg
      901 ccgatgacgg catggttgga ggtggatgtg gcggccggag cagtggctga gctggactgc
      961 tgcgcaaagg cgggcgtgga caggaccagc gccaccgcag cggtggcgag aagggacgcg
     1021 tgacggttca ttggggggat acgcctgttg atacggcgcg gagactggtc ccgccgagac
     1081 gttttgtcgc ggggtaagtg gccgcattac aaaaagctaa tgcaggctgc ggcaaagctc
     1141 ggtcagcacg cgcgggtaga gttgatgctc cgcaacatgg acacgctcgg cgagcgcatc
     1201 gggagtgtcg ccccgcagga tcggcacgcg ggcctgacca agcacaggac cttcgtcgac
     1261 gccgtcggtg acgatgtgga ccgtacatcc gccttcggcg tcgcccgctg caatggcgcg
     1321 ggcgtgggtg tcgagtccgg gatagagcgg cagcagcgac gggtggacgt tgatcatccg
     1381 cccctcccaa cgctggacca gatacggcgt cagaacacgc atataaccgg caagagcgat
     1441 gatctggacg tcggcctcgg ccagaagggc gtcgatggcg cgttcgtgcg cttcgcggtc
     1501 cttggggaag tctcgatgat cgacggcgac ggcccgaacg cctttggcct cggcgatgtg
     1561 caggcctccg gcgtcgggct tgttggagac gaccagcacc acttgatagg ggctgtccgg
     1621 cgtctgaccg gcgtcgatca gggcggccat gttggatccg gcgcccgaga tcatcactgc
     1681 gaccctggtg cggtcgcctg tcgtcgtggg catcaggcgg ctggcggcgt cgggggcgct
     1741 tcggagacga aggcgccggc cgggccgggg aagacgatcg ggctctcgta gccgtcagcc
     1801 gaaaccgacg agacgccgaa gaaatagtcg tcgatcggga tgtcctttag caccagcgtc
     1861 gtggcctcac ccgcccagcg gctgttggtc cactgcggtg cggtcgtcgc acgccaccag
     1921 acgcggtaac ccgcagcgcc cgggacggcg ctccacttga ctgtggtgtc gggcaggacg
     1981 gcgccggtga tggcgacctc ggtcgggacg gtgggggcct tggccatggc ggccatggtg
     2041 acgatgttca gccgggcgac ctgacccaga taggcgaagt cgacgccctc gatggtgtcg
     2101 ccatagacgg tgccgttctc ggtgcggagg tcctgatgct ggcggtcata gttctcggcg
     2161 ccctcggtga tgcggacggc ggggaagccg gccttgagca tttcgacctg atcgccaccg
     2221 cgtccgaacc ggtcggtgcg atagatcatc tcgacatcga aattggtcag gtagcggtcg
     2281 gcgatgctgt cgacgaagcg ggcgaggttg cgcgaggaac tgtccagctc gccgccgtta
     2341 tagcggcgag tcgccgcctg ttcgtgcgtc tcgacggtct tggtgccttc ggagaagacg
     2401 cggatgcggg tcgtgtctcg cacgcccgac tggccttcgg agttaccgac gatgtcgttg
     2461 ttgaggttgg cttcgacgac ccacccttga gccttggcat agtcggccag gatcttgccg
     2521 cccagcaacc cctgctcctc tccggacagg acagcataga cgagggtggc gtcgaacttt
     2581 tgcttcgaca ggacgcgagc ggcttcgagc acggcagcga cgcccgaggc gtcgtcgttg
     2641 gcgcccggcg cgtctgaggt gaagtccatg acgtcggtga cgcggctgtc gatatggccc
     2701 gagatgacga tgacccgctt cgagtcgccg gtgcccttct ggatgcccag aacgttgacg
     2761 acctcggtcg gggtggggac gcggcggcct gtcactgtgt cggcgggcag ggcgatctcg
     2821 atacagccgc cgcattcggt cgagatggtc cgcaactggc tctcggccca gcgtcgggcg
     2881 gcgccgatgc cgcgcgtgtc cgaatcggtc tgcgacaggg tgtgtcgggt gccgaagccg
     2941 accagcgccg tgatgtcgga gcgcatccgc tcaggctgga tctga
//
LOCUS       DASVTM010000189         4944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_765173,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000189 DASVTM010000000
VERSION     DASVTM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765173"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..623
                     /locus_tag="VF633_05205"
     CDS             93..623
                     /locus_tag="VF633_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470489.1"
                     /translation="MSMQARFGRPALAASLTLLAIAAAGCAPTTGGGMYAPPPPSASF
                     NTDAFGWSTRPGSNSIEGRIAFTRNGTAYACTGSVALTPDTPYTRARFHTLYGSTDSA
                     AIPEAIVRARTMADPNADYRSFVRSATCANGRFSLTGLPDGSWFIIAPVSAGGERIVL
                     MRRVETRGGRMISVTL"
     gene            686..1282
                     /locus_tag="VF633_05210"
     CDS             686..1282
                     /locus_tag="VF633_05210"
                     /inference="COORDINATES: protein motif:HMM:NF019501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEX8470490.1"
                     /translation="MSVYSRQISTWAALALAAPLLFAADPSVAQVYGYQPGGAQDFYG
                     YQQAAVCGDRCGRPHYERRYDRSHQGQVDRPGDYRCDAYWDRGRTDCDAGWRDQRRSW
                     RRGDIQRQSPYHADRVVHYGEYGRPDVVYSGGGYGRYSAGASYRDRYGHDHSQDRARY
                     GQGERNPQRINWCCAEYRSYDPATGYYIAYSGEPVFCG"
     gene            1451..1645
                     /locus_tag="VF633_05215"
     CDS             1451..1645
                     /locus_tag="VF633_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470491.1"
                     /translation="MSQHNISGSEAFDRSPSVIIRSVKTGGMRRRRNRTVIAFVAGAG
                     LAIVAGLIGAVAVFGPSLGG"
     gene            complement(1668..2201)
                     /locus_tag="VF633_05220"
     CDS             complement(1668..2201)
                     /locus_tag="VF633_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8470492.1"
                     /translation="MCIIETSPVIETRRLVLRAPAPQDVSRIAAMANDRDIARMTCRM
                     PHPFGTGDAEEFVIAVAGQNPAKASTFLIEHEDAGPVGVIGLFENGDLAPEAGYWIGR
                     DFWGRGFATEALEGALVWASRKWKRRAMMAGHFADNPASGRVLEKAGFLYTGETRKPF
                     SRARQTTADMRMMVWLA"
     gene            complement(2425..2712)
                     /gene="rpmA"
                     /locus_tag="VF633_05225"
     CDS             complement(2425..2712)
                     /gene="rpmA"
                     /locus_tag="VF633_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX8470493.1"
                     /translation="MAHKKSGGSSRNGRDSHSKRLGVKKYGSENVLAGNIIVRQRGTQ
                     FHPGVNVGLGRDHTLFALEHGVVKFTKKANGRCYVSILPANDVAEAAIAAE"
     gene            complement(2798..3343)
                     /gene="rplU"
                     /locus_tag="VF633_05230"
     CDS             complement(2798..3343)
                     /gene="rplU"
                     /locus_tag="VF633_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX8470494.1"
                     /translation="MYAVIKTGGKQYRVQPGDTIVVEKLDGDAGSELKFDSVLMLGGD
                     NGVTLGAPLIDGAFVGATLIETRKGEKVKIFKKTRRQGYRRTNGHRQMESVLRITGID
                     GAGETSKWDGEISLITKAEINLRARGLAKREAGSDLGSTETVVTDHDGAHVDAVVVES
                     SAPAKKAPAKKAAKPAETDEA"
     gene            3592..>4944
                     /locus_tag="VF633_05235"
     CDS             3592..>4944
                     /locus_tag="VF633_05235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8470495.1"
                     /translation="MTTDADSRIIAARRITAHIADLLQADLSLRLWNGEVLPLGPNAR
                     DDIQIVIARPSAVRRLILKPGLMTLFEMFATGDMRIEGGSPLEAADRWDHGRAVHLAK
                     RADKLLIAKMAIPFLLGGSDAGATQLPEWDKTGNDGIGADKAGRSDQDFIQFHYDVGN
                     DFYRLFLDPEMVYSSAYFTDHDATLEAAQEEKLDRICRKLRLQPGQKLLDVGCGWAGL
                     SCWAAKNYGVTVHGVTLSQAQLDFGRAKVKAMGLEDKITLELKDYRHITESGTFDAIS
                     QVEMFEHVGFANHERHFLEMKRLLKPGGLYFHQASVRRGGRDPIKPTDTTKVITRFIF
                     PGGELDTIGMTVTNLGRLGFETLDVEDMREHFELTLKHWEDRLYEKREDAYALVGEAR
                     TRLWLIYFALFKKSFERGSVLVYQTVAQKRRPGPSGLPLDRKSMYAERAVSVRTTIST
                     P"
BASE COUNT          848 a         1624 c         1540 g          932 t
ORIGIN      
        1 gaccttgggg ggcgcgttgg ggttatgact gagatctgtt cgcgcttacc cctgattggg
       61 gaaactgcgg cgctgcgtaa aaaggacccg ccatgtcgat gcaagcgcgt ttcggccgcc
      121 ccgctctggc cgcctccctg acccttctgg cgattgcggc cgcaggctgt gcgccaacca
      181 caggcggcgg catgtacgca ccgcccccgc cgtccgcctc gttcaatact gacgctttcg
      241 gctggtcgac acgtccggga tcgaactcga tcgaaggtcg gatcgctttc acccgtaacg
      301 ggaccgccta tgcctgcacc ggctctgtcg ctctaacgcc tgacaccccc tacacgcggg
      361 caaggttcca cacgctttac ggatcgaccg actccgccgc cattccagag gccatcgttc
      421 gcgcccgcac catggccgat ccgaacgcgg actaccgctc cttcgttcgg tcggcgacct
      481 gcgccaacgg ccgcttcagc ctgaccggcc tgccggacgg cagctggttc atcatcgctc
      541 cggtcagcgc cggaggcgaa cgtatcgtgc tcatgcgccg cgtcgagacg cgcggcggcc
      601 ggatgatctc cgtcaccttg taggcgtaat ccatcaacac ggcggatcgc cctgacgggt
      661 tttcgaccga ctggaggcgt taaccatgtc tgtttattcg cgtcagatct cgacctgggc
      721 cgctcttgcg cttgccgccc cgctgctgtt cgccgctgat ccttcagtag cccaggtcta
      781 tggctaccag cccggagggg cgcaggattt ctatggctat cagcaggctg cggtgtgtgg
      841 cgaccgctgc ggccgccccc actacgagcg ccgctacgac aggagccatc agggtcaggt
      901 cgatcgtccc ggcgactatc gctgcgatgc ctactgggac cggggccgca ccgactgcga
      961 cgccggctgg cgcgaccagc gcaggtcctg gcggcgtggc gacatacagc gccagtcgcc
     1021 gtatcatgcc gaccgggtcg tccattacgg agagtacggt cggccagacg tcgtctattc
     1081 cggtggcggc tatggccgat attccgcagg cgcgagttac cgcgaccgct atggtcacga
     1141 ccattcccag gaccgcgccc ggtatggcca gggcgagcgt aatccccagc ggatcaactg
     1201 gtgctgcgcc gaatatcgtt cctatgaccc ggcgaccggc tattatatcg cctacagcgg
     1261 cgagcctgtc ttctgcggct gatcgagctc gaagaccgac agtcctccga tcacggattg
     1321 gtgggtgagc gacgttcgcc tttgtttggc cccaccgaaa gtcccttgct gaccccgcgt
     1381 ccctttgtca gactcctgag aggcgcgggg tcaattttca gtctgccaac tcagtagggg
     1441 gagcgcgtca atgtcccagc acaacatcag cggttcggaa gccttcgatc gatcgccgtc
     1501 cgtcatcatc cgctcggtca agaccggcgg catgcgtcgc cgtcgcaacc gcacggtcat
     1561 cgccttcgtc gccggcgcag gtttggcgat cgtcgcgggt ctgatcggcg ccgtcgccgt
     1621 gttcggacct agcctcggcg gctaggcccg gcgacaggtc tggccggtca ggccagccag
     1681 accatcatcc gcatgtccgc cgtcgtctgg cgggctcggc tgaacggctt gcgggtttcg
     1741 ccggtgtaga gaaaccccgc cttttccagc acgcgacccg aggccgggtt gtcggcgaaa
     1801 tgcccggcca tcatggcgcg gcgcttccac ttgcggctgg cccagaccag ggcgccttcc
     1861 agcgcctcgg tagcgaagcc ccgcccccag aagtcgcggc cgatccagta gcccgcttca
     1921 ggcgccagat ccccgttctc gaacaggccg atgacgccga caggacctgc gtcctcgtgt
     1981 tcgatcagga aggtggaagc cttggccggg ttctgacccg cgacggcgat aacgaactct
     2041 tcggcgtcac ctgtcccgaa cggatgaggc atgcggcagg tcatccgcgc gatatcgcgg
     2101 tcgtttgcca tggccgcgat acggctcacg tcctgtggtg caggtgcgcg caggaccaga
     2161 cgccgggtct cgatgaccgg ggaggtttcg atgatgcaca tggtgtcgtc gcctcatact
     2221 tgtttggcga atgacgggcg ctgaaaccgg tggccacttt cagctgtcat tcgccgggtc
     2281 ggtccgcctc cttggagggc tcgggcggac ttcaaggcgg ggaacgcaaa cggggagcct
     2341 ggtgatccag actccccgcg gaatatttcc ctggtccgga tcggctgctt tggagagcaa
     2401 cgcgatccga aaaaccggtt cgcgctactc ggcggcgatc gccgcttcgg ccacgtcgtt
     2461 ggccggaaga atggacacgt aacagcggcc attggctttt ttggtgaatt tcaccacgcc
     2521 gtgttcgagc gcgaagagag tgtggtcgcg accaaggccg acgttcacgc ccgggtggaa
     2581 ctgggtaccg cgctgacgca cgatgatgtt gcccgccagc acgttttcgc tgccgtattt
     2641 cttcacgcca aggcgtttgg aatgggagtc gcgaccgtta cgcgacgaac caccggattt
     2701 tttgtgagcc atctgaactc tctcttcgtt gcgctaccgg ccttgcggcc tacttgagcg
     2761 cggtcgtgtg ggtcacgctg cgaagcgcgg gttattctta agcttcgtcg gtctcggcgg
     2821 gctttgcagc cttcttcgcc ggggccttct tggcgggtgc ggagctttcg acgacgactg
     2881 cgtcgacgtg ggcgccgtca tgatcggtga cgaccgtttc agtcgaaccg aggtccgaac
     2941 cggcttcgcg tttagcgagg ccacgggcgc gcaggttgat ttcggccttg gtgatcaggc
     3001 tgatttcgcc gtcccacttg gacgtttcgc cggcgccgtc gatgccggtg atgcgcaaga
     3061 ccgattccat ctggcggtgg ccgttggtac ggcggtagcc ctgacgacgg gtcttcttga
     3121 agatcttgac cttctcaccc ttgcgggttt cgatcagggt cgcgccgacg aaggcaccgt
     3181 cgatcagcgg agcgccgagc gtgacgccgt tatcgccgcc cagcatcagg acgctgtcga
     3241 acttcagctc cgagccagca tcgccgtcca gcttttcgac cacgatcgtg tcgcccggtt
     3301 ggacccggta ctgcttgccg ccggtcttga tcaccgcgta catgttgaag cctcagcgta
     3361 acgcaaaaaa ggatgcgccc gccggatgac ccagcgggcg aagagcggcg acatatacgg
     3421 acagcgacct tcgagtcaac gacaaaccgg tccgcaatcg cctgaaaagc ccgccgcagg
     3481 gcctctgcgc ttcactcccg gcatggcgag cgcttgaaat cgcgaccgtc tctggcgggt
     3541 tgcaacacga ccgtgcaaag aaccgcaaga cgcgaacagg aagaccttca tatgacgacc
     3601 gacgccgaca gccgcatcat cgccgcgcgc cgcatcaccg ctcatatcgc cgacctgctg
     3661 caggctgacc tgtccctgcg tctgtggaac ggtgaggtcc tgccgcttgg acccaatgcg
     3721 cgcgacgaca tacagattgt catcgcccgc ccgtccgccg tgcgccgcct gatcctcaag
     3781 cccggcctga tgaccctgtt cgagatgttc gccacgggcg acatgcggat cgagggcgga
     3841 agcccactgg aagccgccga tcgctgggat catggccgcg ctgtccatct ggccaaacgc
     3901 gccgacaagc ttctcattgc gaagatggcc atccccttcc tgttgggtgg gtccgacgcg
     3961 ggggcgaccc agcttccgga atgggacaag accggcaacg acggcatcgg cgccgacaag
     4021 gccgggcgca gcgaccagga tttcatccag tttcactatg atgtcggcaa cgacttctac
     4081 cggctcttcc tcgatccgga gatggtctac tccagcgcct atttcaccga tcacgacgcg
     4141 acgttggaag cggcccagga agagaagctg gaccgcatct gccgcaagct gcgcctgcag
     4201 ccgggccaga agctgctcga cgtcggctgc ggctgggcgg gcctgtcgtg ctgggcggcg
     4261 aagaactacg gcgtcaccgt tcatggcgtc accctgtcac aggcccagct cgatttcggt
     4321 cgtgccaagg tcaaggccat ggggctcgag gacaagatca ccctcgagct caaggattat
     4381 cgccacatca ccgagagcgg aaccttcgac gccatctcgc aggtggagat gttcgaacac
     4441 gtcggcttcg ccaaccacga acgccacttc ctcgagatga agcgcctgct gaaaccgggc
     4501 ggcctgtatt tccaccaggc ctcggtgcga cgcggcgggc gcgatccaat caagccgacg
     4561 gacacgacca aggtcatcac ccgcttcatc ttcccgggag gagaactgga caccatcggc
     4621 atgacggtga ccaacctcgg ccgtctcggc ttcgagacgc tggacgtcga ggatatgcgc
     4681 gaacatttcg aactgacgct caagcactgg gaagaccgcc tctacgagaa gcgcgaagac
     4741 gcttacgccc tcgtcggcga ggcccgcacc cgtctgtggc tgatctattt cgccctgttc
     4801 aagaagagct tcgagcgcgg ttcggtgctg gtctatcaga cggtggccca gaaacgtcgg
     4861 ccgggtccaa gcggcctgcc gctggatcgc aagtcgatgt acgcggaacg ggcggttagc
     4921 gtccgcacaa caatctcgac gccg
//
LOCUS       DASVTM010000190         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_922285,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000190 DASVTM010000000
VERSION     DASVTM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922285"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF633_05240"
     CDS             <1..323
                     /locus_tag="VF633_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8470496.1"
                     /translation="LLTFIGILAGLLGGMLVTWSQLGYGPAFFLQQMSQDNTMATHLM
                     VGMVKAPVFAIVIAAIGCRQGMSVAGDVESLGRRVTAAVVQAIFAIIFLDAVFALIFL
                     ELNL"
     gene            386..1141
                     /locus_tag="VF633_05245"
     CDS             386..1141
                     /locus_tag="VF633_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8470497.1"
                     /translation="MIEVRDLRSQFGEQVIHEGLNLDVRRGEVLGVVGGSGTGKTVLL
                     NTIIGLKEPDGGAIRIFGHDTTSLTSEESADIERRTGTLFQQGALFSSLSVLENVASP
                     LVEHTRLPRETIRELAEMKIAMVGLKPESHHQKPSELSGGMRKRVGLARALALDPELL
                     FLDEPTAGLDPIGAAAFDDLIRTLSDDLGLTVFMITHDLDSLYAICDKVAVLADKGIV
                     EKATVQELERSDHPWIKEYFLGPRGRAATKTAA"
     gene            1158..2096
                     /locus_tag="VF633_05250"
     CDS             1158..2096
                     /locus_tag="VF633_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8470498.1"
                     /translation="MERDAHYAAVGIATVALLMALAVFTIWLARLQFNKDYDVYDIVF
                     YGPVRGLSEGGEVHFNGIRVGEVTDLNLDPQKGDQVIARVRLNGTTPVRVSSRAQLEP
                     QGITGLNYIQITAGTEGSALLKTQYPENVIPVIQSQPSPIAELLSGSGTVLAQTVDAL
                     NRINRVMSDDNIRSFSTSLKNIETLSTELETRKGMFKELEGALAKANAAVEEYRQLAV
                     DARTVVNGDGRQAIANINKATQDAQVAIASINRSVSGLERPLGEFSTTGLPQLTQTIE
                     ELNEATASLQSLIDDVRASPRDFIGRPASKEIEVQP"
     gene            2093..2743
                     /locus_tag="VF633_05255"
     CDS             2093..2743
                     /locus_tag="VF633_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX8470499.1"
                     /translation="MIPTRLASKTVLAATAIALMLSGCSLLSSPDPVQLYRFGADIQP
                     PASSQATAAQPSSVALRRIDFPEAAKGDRILGVTGNETSYIKGARWVSPAETLFNDSL
                     ENAFNAQPDRVRLISRREPGISTRVLSVNVTTFEARYDVAGGIPTVVITARARMTVLP
                     ERSVADEQVFTVSQPATENRVAAIVSAFDIASRDLNAQIVDWTASANTTPAAIPAR"
     gene            complement(2787..3440)
                     /locus_tag="VF633_05260"
     CDS             complement(2787..3440)
                     /locus_tag="VF633_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX8470500.1"
                     /translation="MARTREQLAERAAQELQDGFYVNLGIGIPTLVANYIPDGMEVTL
                     QSENGMLGMGPFPYAGDEDPDLINAGKQTITEIPESAYFSSADSFAMIRGGHINLSIL
                     GAMEVAENGDIANWMIPGKLVKGMGGAMDLVAGVKRVVVVMDHANKHGESKVLKSCTL
                     PLTGTGVVSRIITDLAVFDVKPDGAGLDLVELAEGVTLDEVAAKTQATYTVSPSLAG"
     gene            complement(3443..>3844)
                     /locus_tag="VF633_05265"
     CDS             complement(3443..>3844)
                     /locus_tag="VF633_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEX8470501.1"
                     /translation="GTLAERIRAGGAGIPAFFTATGVGTLVAEGKEVRNFNGRDYVME
                     TGLVADLAIVKAWKADERGNLVFRKTARNFNPMMATAGKVTIVEVEEIVPTGSLDPDS
                     IHTPGIYVDRLFKGTFEKRIEQRTVRQKEVA"
BASE COUNT          698 a         1234 c         1191 g          721 t
ORIGIN      
        1 cgttgctgac gttcatcggc attctcgccg ggcttttggg cggtatgctg gtgacctgga
       61 gccagctcgg ctacggcccg gccttcttcc tccagcagat gagccaggac aatacgatgg
      121 cgactcacct gatggtgggc atggtcaagg ctccggtgtt cgccatcgtc atcgccgcca
      181 tcggctgtcg gcaaggcatg tcggtcgcgg gcgacgtcga gagcctgggg cgccgggtca
      241 cggccgccgt ggttcaggcc atcttcgcca tcatcttcct cgacgccgtc ttcgccctga
      301 tcttcctgga gctgaacctg tgagcgaggc gaacactccg gacaacgcgc ctgacaaggc
      361 cgctaacgac aagcagcctg aaaacctgat cgaggtgcgc gatctgcgca gccagttcgg
      421 cgagcaggtg atccacgagg gcctgaacct ggacgtccgg cgcggcgagg ttctgggggt
      481 ggttggggga tccggcaccg gcaagaccgt gctgctcaac accatcatag gcctcaagga
      541 gcccgatggc ggtgcgatcc gcatcttcgg gcacgacacg acgagcctca cgtcggaaga
      601 gtcggcggac atcgagcgcc gcaccgggac tttgttccag cagggcgccc tgttctcgtc
      661 gctgagcgta ctcgagaacg tcgcttcccc gttggtcgag cacaccaggc tgccgcgaga
      721 gaccatccgc gaattggccg agatgaagat cgccatggtc ggtctgaagc cggagtctca
      781 ccaccagaag ccatccgaac tatctggcgg catgcgcaaa cgcgtcggtc tggcgcgggc
      841 gctggcgctg gatcccgaac tgctgttcct cgatgagccg actgcggggc tcgacccgat
      901 tggcgcggcg gcgtttgacg acctgatccg gacgctttcg gacgatctgg gcctgacggt
      961 cttcatgatt acccacgacc tcgatagcct ttatgccatc tgcgacaagg tcgcggttct
     1021 ggcggacaag ggtatcgtcg aaaaggccac ggttcaggaa ctggagcgtt ccgatcatcc
     1081 gtggatcaag gaatacttcc tggggcctcg cggacgcgcg gccaccaaga cagccgcctg
     1141 aggagagcgg acccgagatg gaaagagacg cacactacgc cgccgtcggc atcgccaccg
     1201 tcgccctgct tatggcgctg gcggtgttca cgatctggct ggccagactg cagttcaaca
     1261 aggactacga cgtctatgac atcgtgttct acggacccgt gcgtggacta tcagagggcg
     1321 gcgaggtcca cttcaatggt atccgtgtcg gtgaggtcac ggacctgaat ctggacccgc
     1381 agaagggcga tcaggtcata gcgcgtgtgc gcctcaacgg caccaccccg gtccgcgtct
     1441 cctcacgcgc ccagctggaa ccgcaaggca tcaccggact gaactacatt cagatcaccg
     1501 ccggcaccga aggaagcgcc ctgctgaaga cccagtatcc tgaaaacgtc atccccgtga
     1561 tccagagcca gccgtccccg attgcagaat tgctttcggg ctcgggcact gttctggctc
     1621 agacggtaga cgcgctgaac cggatcaatc gggtcatgtc ggatgacaat atccggtcct
     1681 tctcgaccag tctgaagaac atcgagaccc tgtccaccga gttggaaacc cgcaagggga
     1741 tgttcaagga attggaaggt gcgctggcca aggccaatgc cgcggtcgaa gagtaccgtc
     1801 agctggctgt cgacgcccgg acggtcgtga atggagacgg tcgtcaagca atcgcaaaca
     1861 tcaacaaggc gacgcaggac gcgcaggtcg ccatcgcctc gatcaatcga tcggtcagcg
     1921 gtctggaacg gccgctcggg gagttctcga ccacaggtct gccgcaactg acccagacca
     1981 tcgaagaact gaacgaggcc accgcctcgc tgcagagcct catcgatgat gtccgcgcca
     2041 gcccgcgtga cttcatcggt cgtcccgcat ccaaggaaat cgaggtccag ccatgatccc
     2101 gacccgtctt gcttccaaga ctgttctcgc cgccacggct atcgccctga tgctgtcggg
     2161 ttgctcgttg ttgtcctcgc ctgacccggt gcaactgtat cgcttcggcg cggacatcca
     2221 gccaccggct tcatcccagg cgacagccgc gcagccttcg tcagttgcgt tgcgccggat
     2281 cgacttcccg gaggccgcca agggcgatcg tatccttggc gtcaccggta acgaaacgtc
     2341 ttacatcaag ggcgcgcgct gggtttcgcc cgccgaaacc ctgtttaacg acagccttga
     2401 gaacgccttc aacgcccagc ctgacagggt gcgcctcatc agtaggcgcg agcccggcat
     2461 ctcgacacga gtgttgagcg tgaacgtgac caccttcgag gcccgttatg acgtcgccgg
     2521 cggcataccc accgtagtga tcactgcgcg ggctcggatg accgtcttgc ctgagcgttc
     2581 cgtggccgac gagcaggtct tcacggtgtc tcaaccggcc actgagaacc gcgtcgcggc
     2641 catcgtctcg gcgttcgaca tcgcatcgcg cgatctgaac gcccagatcg tcgactggac
     2701 tgcgagcgcc aacaccaccc ccgccgcgat ccccgctcga taaatcggtc gggtgcgccg
     2761 cgggggtggc gtacgccaag cttacatcat cccgccagcg atggtgaaac ggtgtaggtt
     2821 gcctgtgtct tggccgcgac ctcgtccagc gtcacgccct cggccagttc gaccaagtcc
     2881 agacctgcgc cgtccggctt gacgtcgaac acggccagat cggtgatgat gcggctgact
     2941 acgcccgtgc cggtcagggg cagggtgcag gatttcagca ccttggactc gccgtgcttg
     3001 ttggcgtgat ccatgaccac gaccacgcgc ttgacgcctg cgaccagatc catcgccccg
     3061 cccataccct tcaccagctt gccggggatc atccagttgg cgatgtcacc attctcggcc
     3121 acttccatcg cgccgaggat cgagagattg atgtgccctc cacggatcat ggcgaagctg
     3181 tccgccgagc tgaaataggc gctttcggga atctcggtga tcgtctgctt gcctgcgttg
     3241 atcaggtcgg ggtcctcgtc gcccgcgtac gggaacgggc ccatgccaag catgccgttc
     3301 tcagactgga gcgtgacctc catgccgtcg ggaatgtagt tggcgaccag cgtcggaata
     3361 ccgatgccga ggttcacgta gaagccgtcc tgcagttcct gcgcggcgcg ttcggcgagt
     3421 tgttcgcggg tgcgggccat ggttacgcga cctccttctg acggacagta cgttgctcga
     3481 tgcgcttctc gaacgtgccc ttgaacaggc gatcgacgta gattccgggg gtgtggatgc
     3541 tgtccggatc aagcgagccg gtcggcacga tttcctcgac ctcgacgatg gtgaccttgc
     3601 ccgccgtggc catcatcggg ttgaagttgc gggcagtctt gcgaaacacc aggttgccgc
     3661 gctcgtccgc cttccacgcc ttgacgatgg ccagatcggc gacgaggccg gtctccatga
     3721 cgtagtcgcg gccattgaaa ttgcgcactt ccttgccctc ggcgacgagc gtgccgacgc
     3781 cggtggcggt gaagaaggca gggatgcctg cgccgcctgc gcggatgcgc tcggccagtg
     3841 ttcc
//
LOCUS       DASVTM010000191         5237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_73725, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000191 DASVTM010000000
VERSION     DASVTM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73725"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="VF633_05270"
     CDS             complement(<1..1678)
                     /locus_tag="VF633_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX8470502.1"
                     /translation="MDDNYSVQTTAAESAEVGGLDTLQALKDGDTFLVADGWGDLKGG
                     ADGLFDNDTRLLSRFQMTAGAARPSRLSSGVSRDNVFFTAHSTNRPLPPMGGRSAPAG
                     VLHIERRRFVWNRRMFERVRMVNHGIEDVLLPLAFDFAADFADIFQVRGTLREKRGTV
                     HTPTHDGRRVTFRYTGLDDVERQSCLSFSEPPARLSGTRAEFMFSLPMGKRLDLYIEC
                     GGEACSTPDPIRWRENAVAARLSMRSRRRRGASLRGPRSPLFNAWLDQSRADIALLTT
                     DLPTGPYPYAGTPWFSTPFGRDGILTAWQMLWLDPSLAKGVLTYLASRQATEVSAFHD
                     SAPGKIMHETRGGEMSALHEVPFGLYYGGVDTTCLFVGLAGAYAKRTGDLELIRKLWP
                     SLIAATEWMRDYGDSNGDGLIDYARGADTGLSNQGWKDSEDSIFHRDGHFPKGPIALL
                     EVQGYAYAAWLAMADLGARLGDARAQAWEDHAHEVRFLVEDKFWMEDEGFYAIALDGD
                     GEQCRSIGSNAGHLLFSGLPSKDRAARVTRRLLSSEFRSGWGLRTLAKGQA"
     gene            complement(1752..2927)
                     /locus_tag="VF633_05275"
     CDS             complement(1752..2927)
                     /locus_tag="VF633_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8470503.1"
                     /translation="MKIAQITPLYEAVPPKLYGGTERVVAHLTDALVDLGHEVTLFAS
                     ADARTKARLIPVRDQAIRLDPAPLKSDLAAHMTMLAQVLDQADQFDIIHFHTDMIHFP
                     MFRAIADKTVTTLHGRLDMKDLPEVYKRWTEYALISISDDQRRPLSFANWKATVHHGM
                     PAEQYRFSAKSDGYLAFLGRISPEKRPDRAIEIATRLGKRLKLAAKVDAADRVYYEEK
                     IKPLIDGNPLIEFVGEIGDHQKSEFLGGAEALLFPIDWPEPFGLVMIEAMACGTPVIA
                     FKCGSTTEIIEDGATGYLVDTMEQAIAAADRVGLLDREAIRARFELRFSATAMARRYL
                     DVYGDLLAKRPFAEAPLTDVVTPIRPSASREALPLQADPANSALTPVQAEPLIVSAI"
     gene            complement(3072..4622)
                     /locus_tag="VF633_05280"
     CDS             complement(3072..4622)
                     /locus_tag="VF633_05280"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470504.1"
                     /translation="MINAIVRMLPPGSVAWLLAAEVRLGLRGMAGKRGASASKIVLAI
                     ISVIAVAGVVIGGLALGFLASRWPLPVTPLSALIVDAACVVLFTLMLSQTINLAVQAL
                     YERGDLDLLLSSPLKPRVVLTVRALAITVVASILYIGLVTPFVITASVLGRPEWLSLF
                     VVLIALALVATSLGILLTMALFALIGPRKTRVVAQVLAAVVGGVMFALSQVYNLTRNE
                     DGGSSGVTGFMLGLTDSPLLAPDGLLAWPVRALTASPVPLIAIGAIGLILFVAVVQIL
                     GPRFASDAATAVGEKSTPARTKGADGAFADGLSRAMVRKELRLLARDPQLISQILLRL
                     MYLAPLGFVMFRNAHAGTASIFGGAGIVFMAGQLAGSFAWIAISAEDSPDLLRSAPID
                     RGLADRAKLIAALIPTFALTGLAILGFAFLAPLSAIVVAIGCICSAVSSGLVQVWRQK
                     PATRKAFNQRQRGSVLINLVEFIIQGGWAAATALALVGQIWGAVPAVLAILLTLALRR
                     SPEQRWAT"
     gene            complement(4619..>5237)
                     /locus_tag="VF633_05285"
     CDS             complement(4619..>5237)
                     /locus_tag="VF633_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8470505.1"
                     /translation="GKTTTLRMVAGQTKPDSGSIHVFGADAIADPVAAKRLIAWLPDE
                     PMLYDKLTPMEYLEFVAGLWGVDAKVARKRAQELLEFLALWPHRGERCEGFSRGMKQK
                     AALAGALIHEPRLLMLDEPLTGLDAAMARQVKDLLQDRVRAGATVILTTHILDVAERM
                     ADRIGIIQHGKLLAEGTLDELRARSGHGGATLEDVFLDLVGADVS"
BASE COUNT          972 a         1661 c         1770 g          834 t
ORIGIN      
        1 gagcctgacc ctttgcgagg gttctgaggc cccagccgct gcggaactcg ctggacagca
       61 ggcggcgggt gacgcgagcc gcgcggtcct tggacggcaa gccgctgaag agcagatgcc
      121 cggcgttcga gccgatcgag cggcattgct cgccgtcgcc gtcgagggcg atggcgtaga
      181 agccctcgtc ctccatccag aacttgtcct cgaccaggaa gcgaacctca tgggcatgat
      241 cttcccaggc ctgcgctctg gcgtcgccca gccgggcgcc gaggtcagcc atcgccagcc
      301 atgccgcata ggcatagccc tgaacctcca gcagggcgat cggacccttg gggaagtgcc
      361 cgtcgcggtg gaagatcgag tcctcggagt ccttccagcc ctgattggac aggcccgtat
      421 ccgcgccgcg cgcatagtcg atcaggccgt cgccgttgct gtcgccatag tcgcgcatcc
      481 actccgtggc ggcgatcagg ctgggccaga gcttgcggat cagctccaga tcgccggttc
      541 gtttggcata ggcgccggcg agaccgacga acaggcaggt ggtgtcgaca ccgccgtagt
      601 agaggccgaa cggcacctcg tgcagagcgc tcatctcgcc accgcgcgtc tcgtgcatga
      661 tcttgccggg ggcggagtcg tggaaggccg agacctccgt cgcctgacgc gaggccagat
      721 aggtcagcac gcccttggcc agcgacgggt cgagccacag catctgccag gccgtaagga
      781 tgccgtcgcg cccgaacggg gtcgagaacc acggcgtccc cgcatagggg tacgggcccg
      841 tcggcaggtc ggtggtcagc agggcgatgt cggcgcgcga ctgatctagc caggcgttga
      901 acaggggact gcgcgggcca cgcagggagg ccccgcgtcg gcggcgactg cgcatggaga
      961 gccgcgcggc cacggcgttc tcacgccacc ggatgggatc gggcgtcgaa caggcttcgc
     1021 ctccgcactc gatgtacagg tccagccgtt tgcccatggg caagctgaac atgaactcgg
     1081 ctctggtccc gctgaggcgt gcgggaggtt ccgaaaagga caggcagctc tggcgctcga
     1141 catcgtcgag gccggtatag cggaaggtga cccggcgacc gtcgtgggtc ggggtatgaa
     1201 ctgtgccgcg cttctctcgc agggtgccac gcacctgaaa gatgtcggcg aagtcggcgg
     1261 cgaaatcgaa cgccagcggg agcaggacat cctcgatgcc gtggttgacc atccgcaccc
     1321 gctcgaacat gcgccggttc cacacgaaac gacggcgctc gatatgcagc acaccggccg
     1381 gcgctgaacg cccacccatc gggggcaggg ggcggttggt cgaatgggcg gtgaagaaga
     1441 cgttgtctcg cgacacgcct gacgacagcc gcgaaggccg ggccgcgcct gcggtcatct
     1501 ggaaccggga cagcagacgt gtatcgttat cgaacaggcc gtcggccccg cccttcagat
     1561 cgccccagcc gtcggcgacc agaaaggtgt caccgtcctt gagggcctga agggtatcca
     1621 gacctccgac ttcggcgctc tctgcggccg tggtctggac cgaatagtta tcgtccatgg
     1681 gaactccgca tgcggcttcg ggtcgcctga atcgtcccac ccaaatgagg cgcttgctgg
     1741 ccgcttgatc ctcaaatggc tgagacgatc aacggttcag cctggaccgg cgtaagagcg
     1801 gagtttgctg gatcagcctg cagcggcaaa gcttcccggc ttgccgaggg gcggattggc
     1861 gtaacgacat cagtgagagg tgcttccgca aatggccgct tggccagcag gtccccgtag
     1921 acgtcgagat aacgcctcgc catcgccgtt gccgagaagc gcagttcgaa gcgggcgcgt
     1981 atggcttcgc ggtccaggag accgaccctg tcggcggcgg cgatcgcctg ttccatggtg
     2041 tccaccagat agcccgtggc gccgtcttcg atgatctctg tggtcgagcc gcatttgaag
     2101 gcgatgaccg gcgtgccgca ggccatggcc tcgatcatca ccagaccgaa tggctcgggc
     2161 cagtcgatcg ggaacaacag ggcttcggcg ccgccgagga actcggactt ctggtgatcg
     2221 ccgatctcgc cgacgaactc gataagcgga ttgccatcga tcagaggctt gatcttctcc
     2281 tcgtaataga cccggtcggc ggcatcgacc ttggccgcca gcttcaggcg cttgccgagc
     2341 ctggtggcaa tctcgatcgc ccggtccggg cgtttctcgg gcgagatgcg gccgaggaac
     2401 gcgaggtagc cgtccgattt ggctgagaat ctgtactgtt cggcgggcat gccgtggtga
     2461 accgtggcct tccagttggc gaacgacagc ggccgccgct ggtcgtcgga aattgagatc
     2521 aaagcgtact cggtccagcg tttgtagacc tcgggcaggt ccttcatgtc gagccgaccg
     2581 tgcagggtcg tcacggtctt gtcggcgatg gcgcggaaca tcgggaaatg gatcatgtcc
     2641 gtatggaaat ggatgatgtc gaactggtcg gcctggtcca ggacctgggc cagcatcgtc
     2701 atgtgcgcgg cgaggtcaga cttcagtggg gcggggtcga gacggatcgc ttgatcgcgc
     2761 accggtatca ggcgcgcctt tgttcgagcg tctgcactgg cgaataacgt cacctcgtgg
     2821 cccagatcga caagggcgtc agtcagatga gcgacaaccc gctccgtccc accatagagc
     2881 ttcggaggaa cggcctcgta cagaggtgtg atctgggcga tcttcatggt gcgcactccg
     2941 accgcttagc tgggctgtac acatcaatgt tttcgcatat gccaagttcc cgacacagcg
     3001 tacaatttgg tgagggcttg ccgctcttcc gcgagccaaa tcaggcgctc gccgaaggtg
     3061 aaaacgtggt gtcaggtggc ccagcgctgc tctggtgacc ggcgcaaggc gagggtcaac
     3121 aggattgcca gtacggcagg gacggctccc caaatctgac cgacgagcgc gagtgcagtg
     3181 gcggccgccc acccgccctg gatgatgaac tccacgagat tgatcaggac ggagccgcgc
     3241 tggcgttggt tgaaggcctt gcgagtggcg ggtttctgtc gccagacctg aaccaggccc
     3301 gaactgaccg cagagcagat acagccgatc gccacgacga tcgccgacag cggcgccagg
     3361 aaggcgaagc ccaggatcgc cagtccggtc agggcgaacg tcgggatcaa ggctgcgatc
     3421 agcttggcac ggtcggcaag gccacgatca atgggcgcgg aacgcagcag gtcggggctg
     3481 tcctcggccg agatggcgat ccaggcgaag ctcccggcca gctgaccggc catgaagacg
     3541 atgccggcgc cgccaaagat cgacgcagtt cctgcgtggg cgttcctgaa catgacgaac
     3601 ccaagcggcg ccagatacat cagccggagc aggatctggc tgatgagctg gggatcgcgg
     3661 gccagcagtc gcaattcctt cctgaccatc gcgcggctca gtccatccgc gaaagcaccg
     3721 tctgcgcctt tcgttctggc aggcgtagac ttctcgccga cggccgtggc cgcgtcgctg
     3781 gcgaagcggg gaccgagaat ctgcaccacg gcaacgaaca ggatgaggcc gatggcgcca
     3841 atggcgatca agggaacggg gctggcggtc agggcgcgca ctggccaggc gagcaggccg
     3901 tctggtgcga gcagcgggct gtctgtcagg cccagcatga agccggtcac accggatgag
     3961 cccccgtcct cattgcgggt caggttgtac acttggctga gggcgaacat cacgccaccc
     4021 acgaccgccg ccagaacctg agccaccacc cgcgtcttgc gaggaccgat gagggcgaac
     4081 agagccatgg tcaggaggat gccgagcgat gtcgccacca gagccaacgc aatcaggacg
     4141 acgaacagcg acagccattc cggtcggccc agcaccgagg cggtgatcac gaacggcgta
     4201 accagaccga tgtagaggat cgaggcgacc accgtgatgg ccagcgcccg caccgtcaga
     4261 acgacacgcg gcttcaaagg cgaggacagc agcaggtcga gatcgccgcg ctcatacagc
     4321 gcctgaaccg ccagattgat cgtctgggac agcatcaggg tgaacaacac gacgcaggcc
     4381 gcatccacga tcagcgccga cagaggcgtg acggggaggg gccagcgaga ggcgaggaaa
     4441 cccagcgcca gaccgccgat gacgaccccg gcgacggcga tcacgctgat gatcgccagc
     4501 acgatcttcg acgcgctggc gccgcgtttg ccggccatgc cgcgcaggcc gagccgaacc
     4561 tcggcggcca gcagccaggc gacggatccc ggcggcagca tccggacgat ggcgttgatc
     4621 acgagacgtc tgcgcccacg aggtcgagga acacgtcctc cagcgtcgcc ccgccgtgac
     4681 ccgatctcgc gcgcagttca tccagcgtgc cctccgccag aagcttgcca tgctggatga
     4741 tgccgatgcg gtcggccatg cgctcggcga cgtcaagaat gtgggtggtc aggatgacgg
     4801 tggcgccggc ccgcacgcgg tcctgcaaca ggtccttcac ctgacgcgcc atggcggcat
     4861 ccagcccggt caggggctcg tccagcatca gcagtctggg ttcgtggatc agagcgccgg
     4921 ccaacgccgc cttctgcttc atgccgcgcg agaagccctc gcagcgctcg ccgcgatggg
     4981 gccagagggc gagaaactcg agcagttcct gcgcccgctt gcgcgcgacc ttggcgtcaa
     5041 cgccccacag cccggcgacg aactccagat attccatcgg cgtcagcttg tcgtagagca
     5101 tcggctcgtc aggcagccag gcgatgagcc gtttggccgc aacggggtcg gcgatggcgt
     5161 cggctccgaa cacgtggatc gaacccgagt cgggcttggt ctggccggcg accatccgca
     5221 gcgtcgtggt cttgccc
//
LOCUS       DASVTM010000192         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_498049,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000192 DASVTM010000000
VERSION     DASVTM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498049"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VF633_05290"
     CDS             complement(<1..1314)
                     /locus_tag="VF633_05290"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8470506.1"
                     /translation="MIDRRRLMLSAAGVGVVSALPVAGFAQVKPSEAAQSEADARLDA
                     LLTEWFYDDLERHPTQATSLGIDKDEHAHLAGRLADLSLSAAASDKARSEARWAQLKD
                     WPTAGLSDASKVSLAVAQFQAETAAIEAALPFLGSPYVVAQNTGSYFQVPDFMANQHR
                     LESAGDAEAFLSRLEAFAVNVDQENERIRADADEGVMLPAVIMDKTLTQLRALRDMPA
                     ADMAMVQALVRKTGEKSIAGDWGPRAAVIIDAKVKPALGRQIELFESQRPSASEDVGV
                     WRFPHGEALYNLGLRRSTTTTYTGAEIHEIGLKQVASIQAEIATILEGLGMTEGTVGE
                     RVNALGKDPQHLWPNTEEGKVALIASLNEMVAAVTPRLPEVFSTLPKAPVEIRRVPPS
                     IEIGAPGGYYQAASLDGSRAGAYYINLRDTANRPKWSLPTLTYHEA"
     gene            complement(1390..>1892)
                     /locus_tag="VF633_05295"
     CDS             complement(1390..>1892)
                     /locus_tag="VF633_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470507.1"
                     /translation="IAVANLPGAGAAFRIFLPAATAEELVEAAPVVAKVKAAPRDLSG
                     AGRILFVEDEAAVRGIAARLLRQRGYEVIEAADGEEALALAEEWAGQIDMMISDVIMP
                     GLDGPSLLKKARQYLGDAPVMFISGYAESDFSDLLQDEKGVSFLPKPLDIKTLAERVK
                     QELHAD"
BASE COUNT          324 a          603 c          635 g          330 t
ORIGIN      
        1 cgcctcatga taggtcaggg tcggcagcga ccatttcgga cggttcgccg tgtcgcgcag
       61 gttgatgtaa taggcgcccg cccgcgagcc atccagcgac gccgcctgat agtagccgcc
      121 gggtgcgccg atctcgatgg acggcggcac gcgccggatt tccaccggag ccttgggcag
      181 tgtcgagaac acttcaggga gacgcggcgt gacggcggcc accatctcgt taagcgaggc
      241 gatcagggca accttgccct cttcggtgtt gggccagaga tgctgcgggt ccttgcccag
      301 agcgttgacg cgctcgccca ccgtcccttc ggtcataccg aggccttcga ggatcgtggc
      361 gatctcggct tggatggagg cgacctgttt caggccaatc tcatggattt ccgcgccggt
      421 gtatgtggtc gtggtcgaac gacgcaggcc gagattgtag agcgcttccc cgtgcgggaa
      481 acgccagacc ccgacgtcct cagacgcgga agggcgctga ctttcgaaca gctcgatctg
      541 gcggccgagg gccggtttga ccttggcgtc gatgatgaca gcggcgcggg ggccccagtc
      601 tcccgcgatg gatttctcgc ccgtcttgcg gaccagcgcc tgtaccatgg ccatgtccgc
      661 ggccggcatg tcacgcagcg cccggagctg ggtcagggtc ttgtccatga tgacggcggg
      721 cagcatgaca ccttcgtccg cgtcggcgcg gatgcgttcg ttctcctgat cgacgttgac
      781 cgcgaaggcc tcaagccggg acaggaaggc ttcggcatcg cctgctgatt ccagacggtg
      841 ctggttggcc atgaaatccg gcacctggaa gtagctgccg gtgttctgcg ccacgacata
      901 gggcgagcca aggaacggca gcgccgcttc gatggcggct gtttcggcct ggaactgagc
      961 gaccgccagc gaaaccttcg atgcgtcgga caaaccggca gtcggccagt ccttcagctg
     1021 cgcccagcgc gcttcggacc gggccttatc ggatgcagcg gcagagaggc tcagatcggc
     1081 caaccgcccg gcgagatggg cgtgctcgtc cttgtcgata ccaaggcttg tggcctgcgt
     1141 cgggtgtcgc tccagatcgt cgtagaacca ttcggtcagc aaagcgtcca gccgcgcgtc
     1201 cgcttcggac tgtgcggctt ccgatggctt gacctgcgcg aagcccgcga ccggcagggc
     1261 ggaaaccacg ccgacgccgg cggcggacag catcagacgg cgacgatcga tcattgaaag
     1321 ctcctgagac aggaccgcat tggtcagcaa ctcggcagaa ccggcaagcc caaacggcag
     1381 gggacgcgct cagtctgcgt gcagttcctg cttcacgcgt tcggccaggg tcttgatatc
     1441 caacggcttg ggcaggaagg acacgccttt ttcgtcctgc aacaggtcgg agaaatcgct
     1501 ctcggcatag cccgagatga acatcaccgg cgcgtcgccc agatactggc gggctttctt
     1561 gagcagggac ggaccgtcga ggccaggcat gatgacgtcg gagatcatca tgtcgatctg
     1621 acccgcccat tcctcggcca gggcgagagc ttcctcgccg tccgcggctt cgatgacctc
     1681 atagccgcgc tggcgtagca ggcgggcggc tattccccgc accgcggcct cgtcctcgac
     1741 gaacaggatg cggcccgcgc cggacagatc acggggcgca gccttgacct tggctacgac
     1801 cggcgcggcc tcgaccagtt cctcggcggt ggcggcgggc aggaagatgc ggaaagcagc
     1861 gcccgccccg ggcaggttgg cgacagcgat at
//
LOCUS       DASVTM010000193         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_73805, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000193 DASVTM010000000
VERSION     DASVTM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73805"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VF633_05300"
     CDS             complement(<1..586)
                     /locus_tag="VF633_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8470508.1"
                     /translation="MKLPSLFRRTPFRLTLLFLALFAAAASAILAYVYFASASEAEAR
                     ATKDVQVEFDALTAVYNTRGVDALNSALVERTIRGGPYLYLLTDKAGGVVTGNLSVLP
                     FDPGTQTPGMGDWNTFRLTATDEQGHVEDRRSIGVIMPLQSGGDLFVGEDIGDIEESL
                     SRLTQALWLAMGGVLLLGATGGLIISRNVERSMGR"
     gene            complement(596..1267)
                     /locus_tag="VF633_05305"
     CDS             complement(596..1267)
                     /locus_tag="VF633_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8470509.1"
                     /translation="MRVLVVEDDTEAATAMIRGLSEAGHEAAHAVDGAYGLLEAQRGG
                     YDVYVVDRMMPRLDGIGMVETLRKDGDQTPVLFLSAMGEVEDRILGLKAGGDDYLVKP
                     YAFGELIARVEALSRRRETGGVQTVLKVGDLEMNLIARTVYRGTSEIDLQPREFQLLE
                     FLMRHANQSVTRTMLLEKVWEYHFDPQTNVIDVHISRLRSKIDKGFDTAMLQTVRGAG
                     YRLEG"
     gene            complement(1335..>2403)
                     /locus_tag="VF633_05310"
     CDS             complement(1335..>2403)
                     /locus_tag="VF633_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8470510.1"
                     /translation="STDLAVIKVEGSDFNFVSFEEQADPRVGDWVVAVGNPFGLGGTA
                     TAGIVSARGRDIQDRTTSPYVDYLQIDAAINRGNSGGPTFDIYGRVIGVNSAIFSPTG
                     GSVGIGFAIPASTAKSVTDQLMRGGTIERGYLGVEMRTMTDEERSALGLADEVKGVLI
                     NKVTPDGPAEKGGIRAEDVIVAVNGAAVETGNALTRAVANAKVGDELRVELLREGRRQ
                     TVTVRAAKRPTEAELYPTDEEGNANPSAPGTPAVPSGLTVEGLSVSALTPALKSRFSI
                     ADDVSGVVVTDVARGSKAAEGGLPAGTVISRAFSRPVNSPQEFKAAIDAARAAGRPSV
                     LLQVTAQGTTALIALELSKAE"
BASE COUNT          424 a          772 c          784 g          423 t
ORIGIN      
        1 gccgtcccat agaccgctcg acgttacggc tgatgatcaa cccaccggtc gcgcccagca
       61 gcaaaacgcc gcccatcgcc agccacaagg cttgggtcag gcgagacagg gactcctcga
      121 tgtcgccgat gtcctcaccg acgaacaggt cgcccccgct ttgcagaggc atgatgacac
      181 cgatggagcg gcgatcttcg acgtggccct gttcatcggt cgcggtcagg cggaaggtgt
      241 tccagtcgcc catacccggc gtttgcgtcc cgggatcgaa tggcagcacc gagaggttgc
      301 ccgtcacgac ccctcccgcc ttgtcggtca gcagatagag gtaggggccg ccccggatgg
      361 tgcgttcgac cagggcgcta ttcagggcgt cgaccccgcg cgtgttgtag acggcagtca
      421 gggcgtcgaa ctcgacctgc acatccttcg ttgcgcgcgc ttccgcctcc gacgccgacg
      481 cgaaataaac gtaggccagg atcgcgctgg ccgccgctgc gaacagggcg aggaacagca
      541 gcgtcagccg gaagggcgtg cggcgaaaga gggagggaag cttcatcgag gcgtcttagc
      601 cctcgagccg ataccctgcg ccgcgcaccg tctgcagcat cgcggtgtcg aagcccttgt
      661 cgatcttgga acgtaagcgg ctgatgtgga cgtcgatgac gttggtctgg gggtcgaaat
      721 ggtattccca gaccttctcc agcaacatgg tgcgggtgac cgactggttg gcgtgacgca
      781 tcaggaattc cagcaactgg aactcacggg gctgaagatc gatctccgac gtgccgcgat
      841 agacggtgcg ggcgatgagg ttcatctcga ggtcaccgac cttgagtacg gtctggacgc
      901 cgccggtctc gcgacgacgc gacagggcct cgacgcgggc gatgagctcg ccgaaagcat
      961 aaggcttgac cagatagtcg tcgccaccgg ctttcaggcc gaggatgcga tcctcgacct
     1021 cacccatggc ggacaggaac aggaccggcg tctgatcgcc gtccttgcgc agggtctcga
     1081 ccatgccgat gccgtcaaga cggggcatca tgcggtcgac gacatagacg tcataaccgc
     1141 cgcgctgagc ctcgagcaga ccgtaggccc cgtcgacagc atgagccgcc tcgtgtcccg
     1201 cctcgctcag accgcggatc atcgccgtcg ccgcttcggt gtcgtcctcg actaccagaa
     1261 cacgcatgga accctcctga attcagaatc gtcacggacg ttagcgcatc cgtgacgatc
     1321 ctgtcagtta aggcttattc agctttggaa agctcgagag cgatcagagc cgtggtgcct
     1381 tgcgctgtga cctgcagcag gacgctgggg cgaccggcgg cccgggcggc gtcgatcgca
     1441 gccttgaact cctgaggcga gttgaccggc cggctgaagg cgcggctgat cacggtgcct
     1501 gctggaagcc cgccttcggc cgccttggat ccacgcgcta cgtcggtcac gaccacgccg
     1561 gacacatcgt ccgcgatgct gaaccggctc ttgagcgcgg gggtcagggc cgagaccgag
     1621 aggccttcga ccgtcagacc cgacggaacg gctggcgtcc cgggagcgct ggggttggcg
     1681 ttgccttcct cgtcggtcgg atagagctca gcctcggtcg gacgtttggc ggcgcggaca
     1741 gtcaccgtct ggcggcggcc ctcacgcaga agctcgacgc gaagctcgtc gccgactttg
     1801 gcgttggcca cggcgcgggt cagggcgttg ccggtctcta cagccgctcc gttgacggcg
     1861 acgatgacgt cctcggcgcg gataccgccc ttttcagccg gaccatcggg cgtgactttg
     1921 ttgatcagca cacctttgac ttcgtcggcc agccccagag cgctgcgttc ttcatccgtc
     1981 atcgtgcgca tctcgacacc gagatagccg cgctcgatcg tgccaccgcg catcagttgg
     2041 tcggtgacgc tcttggccgt tgaggctggg atggcgaaac cgataccgac cgacccgccg
     2101 gtgggcgaga agatcgccga gttcacgccg attacgcgac catagatgtc gaacgtcggg
     2161 ccacccgagt tgccgcggtt gatggcggcg tcgatctgga gatagtcaac gtaaggcgat
     2221 gtcgtgcggt cctgaatgtc gcggcctcgc gctgagacga tgcctgccgt cgcagtgccg
     2281 ccgagaccga acgggttgcc gaccgcgacg acccagtcgc caacgcgcgg atcggcctgt
     2341 tcctcgaagc tgacgaagtt gaagtccgaa ccctcgacct tgatcacggc cagatcggtg
     2401 ctt
//
LOCUS       DASVTM010000194         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_285794,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000194 DASVTM010000000
VERSION     DASVTM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285794"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF633_05315"
     CDS             <1..459
                     /locus_tag="VF633_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX8470511.1"
                     /translation="QSCHLVVPSEDQIVVIARIESPGDLGYSVRIGYRRNIVDATSGL
                     AFYGWAEPQTRAHLHAVLVAGSGARKVAQFAAMATEATRNDYIERASDFVKGVTDLVA
                     PIRGANGIIATLITPFIEHAPAVCSKDEAARLLRQAADTISEQMGAVTKA"
     gene            577..1335
                     /locus_tag="VF633_05320"
     CDS             577..1335
                     /locus_tag="VF633_05320"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8470512.1"
                     /translation="MGAFYPDLRDQVVIVTGGAGGIGEAITRAFVAQGCRVGFLDIDQ
                     ERGRRVQAEITAAGGSALFVECDLTRIPDVKAAVQSIREAFGPIDILINNAAHDERHE
                     TLEVSEDYWDARMAVNLKHQFFAAQAVLPDMKASGKGSIVNLGSTSWIIGQGGMAAYT
                     ACKSAVIGLTRSLARDFGQFGVRVNAVAPGWIMTERQLELWVTPETEKEIYANQCLKR
                     RLVPDDIARVIVFMSSDEAGAITNQHYVVDGGWT"
     gene            1338..3134
                     /locus_tag="VF633_05325"
     CDS             1338..3134
                     /locus_tag="VF633_05325"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX8470513.1"
                     /translation="MSVDSKKGLRSRAWFNNPDNPDMTALYLERYLNYGLTRRELQSD
                     KPIIGIAQTGSDLSPCNRHHLVLAERVREGIREAGGIAMEFPVHPIQETGKRPTAGLD
                     RNLAYLGLVESLYGYPLDGVVLTIGCDKTTPACLMAAATVDMPAIALSVGPMLNGWHR
                     GERTGSGTIIWKARQMMAAGEIDYEGFIELVASSAPSVGYCNTMGTATTMNSLAEALG
                     MSLPGSAAIPAPYRERAQVAYETGLRIVEMVREDLKPSDILTKEAFQNAIVVNSAIGG
                     STNAPIHLTALARHAHVDVPLQDWQTHGLHVPLLVNLQPAGAYLGEDYFHAGGVPAVM
                     AELMKQGLINEGALTVTGRSIGDNCRDAEIVLPDVIRTFDNPLKTDAGFVVFTGNLFN
                     SAIMKLSVISPEFRERYLSNPDDLNAFEGPAVVFDGPEDYHRRIDDESLGITEKSLLF
                     MRGAGPVGYPGAAEVVNMRAPDYLIKTGVTALACIGDGRQSGTSGSPSILNASPEAAT
                     GGNIALLRTGDRVRIDLNTGRADVLISPEELTERRVAFEASGGYAYPASQTPWQEIQR
                     GMVSELETGAVLEPAVKYHRIIDRFGLPRDSH"
     gene            complement(3165..>3663)
                     /locus_tag="VF633_05330"
     CDS             complement(3165..>3663)
                     /locus_tag="VF633_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8470514.1"
                     /translation="EAGLTGALYRYAQGQITRHDDGYRVTNGPAMSPDGRTLYHNDTT
                     EGRIYAFDHADGVLTGKRLFAETPGVGADGPCVDAAGVVHVGMFNGWGVARFDPDGVR
                     LEPIRFPAQTVTKAAFAGDDLRDLYCTTAWLGNADKRTDQPGLGGLYRVRVETPGLPQ
                     ALARL"
BASE COUNT          668 a         1177 c         1162 g          656 t
ORIGIN      
        1 cagtcctgcc atctcgtggt cccttccgaa gaccagatcg tcgtcattgc gcgcatcgaa
       61 agccctggcg atctggggta ctccgtgagg atcggatatc ggcgcaatat cgtcgacgcc
      121 acctctggtc tggccttcta cggctgggcc gaaccacaga cacgcgcgca tcttcacgca
      181 gttctggtgg ctggctcggg cgctcggaag gtcgcgcagt tcgccgccat ggccacggag
      241 gcgacgcgca acgactacat cgaacgggcc agcgatttcg tgaagggcgt gaccgatctc
      301 gtcgccccga tccgtggggc taacggcatt atcgcaactc tgatcacgcc ctttatcgaa
      361 cacgccccgg cagtctgcag caaggacgag gccgcccggc ttttgaggca ggccgccgat
      421 acgatttcgg aacagatggg cgctgtcacc aaggcttgac cgcgtcaccg ttgacatcgc
      481 gcccgccact agtcattttt gccaacgaga acgcgtttca cttgtgaaat gcagtaggtc
      541 gcagcagagc ggctggtgtc ggagccgtag gcgatcatgg gcgcttttta tccagaccta
      601 agagatcagg tcgtcattgt gaccggcggc gccggcggta tcggcgaggc cataacgcgc
      661 gcctttgtcg cgcagggttg tcgcgttgga tttctggaca tcgaccaaga gcgcggacgc
      721 cgcgttcaag ccgagattac agcggccggt ggcagcgccc tgtttgttga atgcgatctt
      781 acccgaattc ccgacgtgaa ggcggccgtc cagtccatac gggaagcctt tggcccaatc
      841 gacatcctga ttaacaacgc cgctcacgac gaacgtcacg aaacgcttga ggtcagcgaa
      901 gactattggg acgcccggat ggcggtgaat ctgaagcacc agttcttcgc cgcccaagcc
      961 gtgttgcccg acatgaaggc gagcggcaaa gggtcgatcg tcaatctggg ttcgacctcc
     1021 tggatcatcg gccagggtgg aatggcggcc tataccgcct gcaaatcggc cgtgatcggg
     1081 ctgacacgtt cactggcgcg ggatttcggc cagttcggcg tccgcgtgaa cgccgtcgcg
     1141 cccggctgga tcatgactga gcggcagctg gagctctggg tcacccctga gaccgaaaaa
     1201 gagatctacg ccaaccagtg cctcaaacgc aggcttgtcc ccgacgatat cgcacgggtg
     1261 atcgtgttca tgagttcgga cgaagccggc gcaatcacca atcaacatta cgtcgtcgat
     1321 ggcggctgga cctgacaatg agcgtcgatt cgaagaaggg tctgcgtagc cgggcctggt
     1381 tcaacaaccc tgacaacccg gacatgacgg cgctctatct ggaacgctat ctgaactacg
     1441 gactgacccg gcgcgagctg cagtccgaca agccgatcat tggtatcgcc cagacgggat
     1501 cggatctcag cccttgcaat cgccaccatc tcgtgctggc cgaacgggtc cgcgaaggca
     1561 tccgcgaagc cggtggtatc gcgatggaat tcccggttca tcccatccag gagaccggca
     1621 agcggccgac cgccggtctg gaccgaaacc tggcctatct cggtctggtc gagagcctct
     1681 acggctatcc tctggacggc gtcgtcctga ccataggctg cgacaagacg acaccggcct
     1741 gtctgatggc cgccgccacc gtggacatgc cggctatcgc actttcggtc gggccgatgc
     1801 tcaatggctg gcaccggggc gagcgcaccg gctccggcac gatcatctgg aaggcgcgcc
     1861 agatgatggc ggccggagag atcgactacg agggcttcat cgagctggtg gcctcctccg
     1921 ccccgtccgt ggggtattgc aacaccatgg gcacggcgac gacgatgaac tcgctggccg
     1981 aggcgctcgg catgtccctg cccggctccg cagcgatccc cgccccgtat cgcgagcggg
     2041 cgcaggtcgc ctatgagacc ggcttgcgaa tcgtggagat ggtgcgtgaa gacctgaagc
     2101 cttccgacat cctgaccaag gaagcgtttc agaacgcgat cgtggtcaac tcggccatcg
     2161 gcggctcgac caacgcgccg atccatctga ccgctctggc tcgccacgcc catgtggatg
     2221 tgccccttca ggactggcag acccatggcc tgcatgtgcc gctgctggtc aatctgcagc
     2281 ccgccggcgc ctatctcggc gaggactatt tccatgccgg cggcgtacct gcggtcatgg
     2341 ccgagctgat gaagcagggc ctgatcaacg agggcgccct gaccgtcact ggccgctcga
     2401 tcggcgataa ctgccgtgac gccgagatcg ttctgcccga cgtgatcagg acgttcgaca
     2461 accctctgaa gaccgacgcc ggcttcgtgg tgttcaccgg caacctgttc aactcggcga
     2521 tcatgaagct gagcgtgatc agtcccgagt tccgtgaacg ctatctgtcc aatcccgacg
     2581 acctgaacgc cttcgagggg cccgcagtcg tgttcgacgg gccggaagat tatcaccgcc
     2641 gtatcgacga tgagagcctc ggcatcaccg aaaagagcct gctgttcatg cggggtgcgg
     2701 gcccggtcgg ctatccgggc gcggcagagg tcgtgaacat gcgcgcgccg gattacctca
     2761 tcaagacggg cgtcacggcg ctggcctgta tcggcgacgg gcgccaatcg ggtacgtcag
     2821 gctcaccatc gatcctgaac gcatccccgg aggcggcgac cggaggcaat atcgccttgt
     2881 tgaggacagg cgaccgggtg cggatcgacc tgaacaccgg gcgggccgac gtcctgatct
     2941 cccctgagga actgaccgag cggcgggtcg cgttcgaggc gtcgggcggc tacgcctacc
     3001 cggcctctca gacgccttgg caagagatcc aacggggcat ggtcagcgaa ctcgagaccg
     3061 gcgctgtgct ggagccggcg gtcaaatatc accgcatcat cgaccgcttc ggcttgcctc
     3121 gggacagcca ctagaacgct gggtgtctgg cgtcctgttt ccgcctatag ccgcgcaagg
     3181 gcctgaggaa ggccgggcgt ctccacccga acccggtaga ggccgccgag gccgggctgg
     3241 tccgtccgct tgtcggcgtt tccgagccag gcggtcgtgc aatagaggtc gcgcaggtcg
     3301 tctccggcga acgcggcctt ggtcacggtc tgggccggaa acctgatcgg ctccagccgc
     3361 acgccgtcag ggtcgaaacg cgccacgccc cagccgttga acatcccgac gtggaccact
     3421 ccggcggcat ccacgcacgg cccgtcggct ccaacccccg gcgtctcggc gaacaggcgt
     3481 ttgccggtca gcacgccgtc agcatgatcg aaggcgtaga tccggccctc cgtggtgtcg
     3541 ttgtgataga gcgtccgccc gtcggggctc atcgccggtc cgttggtgac gcggtagccg
     3601 tcgtcgtggc gcgtgatttg accttgggca tagcgataga gcgcgcccgt caaacccgcc
     3661 tcg
//
LOCUS       DASVTM010000195         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_285867,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000195 DASVTM010000000
VERSION     DASVTM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285867"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..979)
                     /locus_tag="VF633_05335"
     CDS             complement(251..979)
                     /locus_tag="VF633_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX8470515.1"
                     /translation="MRSILDFFLNMEAKRWRALVATLVLFAVIVALFAVGKSTLGLGQ
                     GAEGAEARLEDWLVGFRSGPWGLLAAIVLFTLSAFLGVPQFILIAACVVAFGPWFGFG
                     YSWIATVASAAVTYWLGRGPTARLVERFGGKTMGRMTRFVGQNAFYTSFMIRNVPSAP
                     FIVVNMAFGAARASFPGFLAGCALGVLPKTALVAFFGGSFMTAVRGDGVWTSLILASV
                     AVVWLALMLAVREIVKRREAARGD"
     gene            1098..2321
                     /locus_tag="VF633_05340"
     CDS             1098..2321
                     /locus_tag="VF633_05340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX8470516.1"
                     /translation="MSTLQSSAPLQSSALARVKPSATIAVSAKARALAAAGRDIIGLG
                     AGEPDFDTPDNIKAAAIKAINDGQTKYTDADGMPSLKKAIADKFARENGLTYDVSQIH
                     VAPGGKPVIFNAFVATLNAGDEVIVPAPYWVSYPDMVLLAGGEPVFVTGEEADGFKLR
                     PEVLEAAITDRTKWLILNSPSNPTGAAYTRAELQALADVLLRHPQVWVLTDDMYEHLT
                     FGDFEYTTIAQIEPKLYDRTLTVNGVSKAYAMTGWRIGYAGGPKALIDLMRKVASQTT
                     SNPTSISQFAAVEALNGPQDFLAPRKEAFEKRRDLVVSMLNQATGIRCANPEGAFYVY
                     PSIDGLIGKTTPSGVVITDDEAFTSELLEAEGVAVVQGAAFGLSPYFRISYALSEAVL
                     EEACSRIQRFAASLT"
     gene            2441..2569
                     /locus_tag="VF633_05345"
     CDS             2441..2569
                     /locus_tag="VF633_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470517.1"
                     /translation="MVQDYREGRHAFSPAGVAGLLLAGMVCGVFWREIVHLILSLG"
     gene            complement(2594..>3217)
                     /locus_tag="VF633_05350"
     CDS             complement(2594..>3217)
                     /locus_tag="VF633_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX8470518.1"
                     /translation="RAFELMVETGQMRALGILATASSVIAEGEVLQLMRAHDLNLDQD
                     TYLQIISAKTAELFAAAAEAGAVGAGADDSATAALRSYGLNLGLAFQLADDALDYGGS
                     SEALGKNAGDDFREGKVTLPLLLAMARTRGREDAFWERTINKGERTEDDFRRARELII
                     GTGAVTATLDTAGDYADTAKAALRVLPRSDWRDALDALADFAVSRAA"
BASE COUNT          613 a         1136 c          937 g          531 t
ORIGIN      
        1 ccccgccgta cgcatcccca tacgcacaac atactgatcg tctttcgcct gaccgactgt
       61 ggcccgtttg ccctcatggg atggcgatta caagtcctca tccccccttt ggcgagatgt
      121 gcgatcacgt cctggttacg gccacgcttg ggttggggcg gggtcgtgag cattcaaagc
      181 cgtcaggcca cgctcgcccg gctatggcag cgaaaccgtg cacggacaaa gggcctgaaa
      241 gtcggagatg tcagtcgccg cgcgcggctt ctcgccgctt cacgatctcg cgtaccgcca
      301 gcatcaatgc cagccagacc acggcaacgc tcgcgagaat cagcgaggtc cagactccat
      361 cacccctcac ggccgtcatg aaggatcctc cgaagaacgc taccagcgcc gtcttgggca
      421 acacgcccag tgcgcagccc gccagaaacc ccgggaacga cgcgcgcgcc gcgccgaacg
      481 ccatattgac gacaatgaac ggcgctgacg gcacgttgcg gatcatgaag gacgtataga
      541 aggcgttctg gccgacgaac cgcgtcatac gccccatggt cttgccgccg aaccgctcaa
      601 ccagtcgcgc cgtcggccct ctccccagcc agtatgtcac cgccgctgaa gccaccgtcg
      661 cgatccagct ataaccaaac ccgaaccacg gcccgaacgc cacgacacag gcggcgatca
      721 ggatgaattg cggcacgccg agaaacgccg acagcgtgaa cagcacgatc gcggcaagca
      781 ggccccacgg cccgctgcgg aacccgacca gccagtcctc cagccgcgcc tcggcgccct
      841 cagcgccctg gcccaatccc aacgtcgact tgccgaccgc gaacaacgcc acgatcaccg
      901 cgaacagcac cagcgtcgcc accagcgccc gccagcgttt ggcctccata ttgaggaaga
      961 aatcgaggat ggaacgcatg atccggcccg aatagcaccg cccggtgcat ttcggcgagc
     1021 ctcgcggttt tcagagggcc ttcatctgcg taaagaggcc cgccgcctcc tctcccttgc
     1081 ggttccggga ttcgcctatg tccacgcttc agtcgtccgc gccccttcag tcatccgccc
     1141 ttgcccgcgt caaaccgtcc gccacgatcg cggtcagcgc caaggcccgc gcgctcgccg
     1201 ccgctggtcg cgacatcatc ggcctcggcg cgggtgagcc cgacttcgac accccggaca
     1261 atatcaaggc ggccgccatc aaggcgatca acgacggcca gaccaaatac accgacgccg
     1321 acggcatgcc ttcgctcaag aaggccatcg cggacaagtt cgcgcgggaa aacggcctga
     1381 cttacgacgt gtcgcagatc cacgtcgctc ccggcgggaa gcctgtcatc ttcaacgcct
     1441 tcgtcgctac tctgaacgcc ggtgacgaag tcatcgtgcc cgcgccgtat tgggtttcct
     1501 atcccgacat ggtgctgctg gcgggcggcg agcccgtgtt cgtgaccggt gaggaagccg
     1561 atggcttcaa gctccgcccc gaagtgctag aggctgcaat caccgatcgc accaagtggc
     1621 tgatcctcaa ctcgccgtcc aacccgaccg gcgccgccta cacccgcgca gaactccagg
     1681 ccctggccga cgtgcttctg cgccatccgc aggtctgggt cctgaccgac gacatgtacg
     1741 agcacctgac gttcggcgac ttcgaataca ccaccatcgc ccagatcgaa ccgaagctat
     1801 acgaccggac attgacggtg aacggcgtct ccaaggccta cgccatgacc ggctggcgca
     1861 tcggctatgc gggcggcccc aaggccctga tcgacctgat gcgcaaggtc gccagccaaa
     1921 ctacatccaa tccgacctcg atcagccagt tcgccgccgt cgaggccctg aacggcccac
     1981 aggacttcct cgccccccgc aaggaggcct tcgagaagcg ccgcgatctg gtcgtgtcga
     2041 tgctcaatca ggcgaccggc atccgttgcg ccaatccgga aggcgccttt tacgtctatc
     2101 cgtccattga cgggctgatc ggcaagacca cgccatccgg cgtcgtcatc accgacgacg
     2161 aggccttcac ctcggaactg ctggaagctg aaggcgtggc ggtggttcag ggcgcagcct
     2221 tcggcctcag cccctatttc cgcatcagct acgccctgtc ggaagccgtg ctcgaagaag
     2281 cctgcagccg catccagcgg ttcgccgcca gcctgacttg aaagccagcc cgacctgatt
     2341 tgtacggagc aaaagccgag cttgcgttca catcaaacgg gtctagattc gtagttaatg
     2401 cacgagagcc gactcaagac ggcccgttcg ggagagagac atggtccagg attatcgtga
     2461 ggggcgacac gccttctcgc cagcaggtgt ggcgggcctg ttgctcgcag gtatggtctg
     2521 cggcgtattc tggcgcgaga tcgtccatct catcctcagc ctgggctgaa gacggtcgaa
     2581 accgactgtg cgatcaggcc gctcgggaga ccgcgaagtc ggctaacgca tccagcgcat
     2641 cgcgccagtc gctacgggga aggacgcgca acgcggcttt ggccgtatcc gcatagtcgc
     2701 ctgccgtgtc cagcgtggcg gtgacggcgc cggtgccaat tatcagttcg cgcgcacggc
     2761 ggaaatcgtc ttccgtgcgc tcgcccttgt tgatcgtccg ttcccagaag gcgtcctcgc
     2821 gcccgcgcgt gcgggccatg gccagcagca acggcagggt gaccttcccc tcacggaagt
     2881 cgtcgccggc gttcttgccc agagcttcgc tggaaccgcc gtagtcgagc gcgtcgtcgg
     2941 ccagctgaaa cgccagcccg aggttcaatc catatgatcg aagcgctgcg gtggcggaat
     3001 cgtcggcgcc tgccccgacc gcgcctgcct cggccgcggc ggcgaaaagt tcggcggtct
     3061 tggccgatat aatctgaaga taggtgtcct gatcgaggtt caggtcgtgg gcgcgcatca
     3121 gctgcagcac ctcgccctcg gcgatgacgc tggacgccgt cgccagaatt cccagcgccc
     3181 gcatctggcc ggtctcgacc atcagctcaa acgcccg
//
LOCUS       DASVTM010000196         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_443182,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000196 DASVTM010000000
VERSION     DASVTM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443182"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VF633_05355"
     CDS             <1..260
                     /locus_tag="VF633_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX8470519.1"
                     /translation="SRIGSAADSLQRIKQRQAVNEEKLKAGQELEDRRTGADLDAKLK
                     EAGILPGHSSADDVLARLTQQEVRVVTPQIGTSVPADKDPA"
     gene            440..1603
                     /locus_tag="VF633_05360"
     CDS             440..1603
                     /locus_tag="VF633_05360"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX8470520.1"
                     /translation="MQRIKLPPRPDWKAKAEAVGFTWSQADGQEYWDESAVYEFSLAE
                     IEDGIEAPTAELHSMCLDLVDEAVQSERLMAQLEIPEGTRDYVAESWKRNEPSLYGRF
                     DFAYDGTGPAKLYEYNADTPTSVYETAVFQWLWLEDQIAAGVFPEDTDQFNSLHEKLV
                     DRFREIFPEGGFVHFASDPDYVEDRQTVKFLEDLATQAGLEPQFVAIGDIGLNEDGKF
                     VDHENFLIGAMFKLYPWEQMLRDDYAEPLPGADVTVLEPAWKSLLSNKGILPLLWERH
                     AGHPNLLESYFETDPAHTTLGSSYVRKPLFSREGANVELVKDGRKSSVLDGGYGDGAW
                     IRQALHAPPKFDNRYVIVGSWLVGNEPAGIGLREDRGRITRNTSRFLPHIIRG"
     gene            1763..>2967
                     /locus_tag="VF633_05365"
     CDS             1763..>2967
                     /locus_tag="VF633_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8470521.1"
                     /translation="MSNIAILAALGAGLFSASKVKAEGQDANGAVVRSVEEHADMIEA
                     LIAQMTIEEKAGQLNLLADPFRFRPQNVNPLDGQGDPAKVRDAIRSGQCGALFNGVGA
                     EAGRTIQRIAMEETRLKIPLLFAADIIHGVYTVFPVPLAEASAFDADLAQRTARAMSL
                     EGAVSAIHQTYAPMVDVARDQRWGRVVEGAGEDVYLNEVLAAARVRGVQGEKGLADRD
                     ALLATPKHFVAYSAAIGGMEYNTTDMSEATLRGVFLPPFKAALDAGALSVMSAFNDLN
                     GVPTSGNHRMLTDVLRTEWGFEGFVVSDYTSENELIAHGFAEDGRDAARIALLAGMDM
                     SMVSGLFLEHIPDLVEKGDVPLARVDQAVRRVLMTKAALGLFDDPYRGCDPERERLVV
                     GSREHRDLAR"
BASE COUNT          527 a          951 c         1008 g          481 t
ORIGIN      
        1 agtcgcgcat cggctcggcc gccgacagcc tgcaacgcat caagcagcgt caggccgtca
       61 acgaagagaa gctgaaggcc ggtcaggagc tggaagaccg ccgcacaggg gcggacttgg
      121 atgccaagct gaaggaggcc gggatcctgc ccgggcattc gtcggccgat gacgtactgg
      181 cgcggctgac gcagcaggag gtccgggtgg tgacgccgca gatcgggacc agcgtcccgg
      241 cggataagga cccggcttaa gaacttgtct cctccacgtt cgccgcttgg cgaatgggga
      301 ggtggcgcgg cgctcctcgg cgccgtgacg gaggggttct tgaactccca cgatcgcctg
      361 cgcgggaaga gcccctccac cgcttcgcgg tccccctccc aatcgccaag cggcgattgg
      421 gaggagactt gagcacccaa tgcaacgcat caaacttccg ccccgtcccg actggaaggc
      481 caaggccgag gcggtcgggt ttacctggag ccaggccgac ggtcaggaat actgggacga
      541 atccgccgtc tatgagttct cgctggccga gatcgaggac gggatcgagg cgccgacggc
      601 cgagttgcat tcgatgtgcc tcgatctggt cgatgaggcg gtccagtccg agcggctgat
      661 ggcgcagctc gagattcccg agggcacgcg cgactatgtc gccgagagct ggaaacggaa
      721 cgagccgtcg ctgtatggcc ggttcgactt cgcctatgac ggaaccggtc cggccaagct
      781 gtacgagtac aatgccgaca ccccgacctc ggtctatgag acggcggtgt tccagtggct
      841 gtggctcgag gatcagatcg cggcgggcgt cttccccgag gacaccgacc agttcaacag
      901 cctgcacgaa aagctggtcg accggttccg tgagatattc ccggaaggcg gcttcgtgca
      961 tttcgcctcc gaccccgact atgtcgagga ccgccagacg gtgaaattcc tcgaggatct
     1021 ggcgacgcag gccgggctgg agccgcagtt cgtggccatc ggcgacatcg ggctgaacga
     1081 ggacggcaag ttcgtcgacc acgaaaactt cctgatcggg gcgatgttca agctctatcc
     1141 gtgggagcaa atgctgcgcg acgactacgc cgagccgctg ccgggcgccg acgtcacggt
     1201 gctggaacca gcgtggaaat cgctgctgtc gaacaagggc atcctgccgc tgctctggga
     1261 gcgtcacgcc gggcatccaa acctgctgga aagctatttc gagacggatc ctgcgcatac
     1321 gacgctgggc tcgtcctatg tgcgcaagcc gctgttctcg cgcgagggcg ccaacgtcga
     1381 actggtcaag gacgggcgca agtcgtcggt gctggacggc ggttacggcg acggggcgtg
     1441 gatccgtcag gcgctgcacg ccccgccgaa gttcgacaat cgctacgtca tcgtcggctc
     1501 ctggctggtc ggaaacgaac cggcgggcat tggtctgcgg gaggatcgcg ggaggatcac
     1561 gcgcaacact tcgcgcttcc tgccacacat catccggggg tgacccggag agagcgaaac
     1621 catcggtgac ctgacataag cgtcagcggc ggctaggaac cccgtgcccc cgatgaaaag
     1681 gtccgctatg tcgatgtcca cgaccgcccc ccggaattca cggggagcga attctcccgc
     1741 ccagatgtcc cgccggtccg caatgtccaa tatcgccatc ctcgcggccc tcggcgccgg
     1801 cctgttctcc gcttccaagg tgaaggccga aggccaagac gccaatgggg ccgtcgtgcg
     1861 gtcggttgag gaacatgccg acatgatcga ggcgctgatc gcgcagatga cgatcgagga
     1921 gaaggcgggg cagctcaacc tgctggccga tccgttccgc ttccggcccc agaacgtcaa
     1981 tccgctggac ggacagggtg atcccgcgaa ggtccgcgac gccatccgct cgggccagtg
     2041 cggcgctctg ttcaacggcg tcggcgcaga ggccgggcgt acgatccagc gcattgcaat
     2101 ggaagagacg cggctgaaga tcccgctgct gttcgccgcc gacatcatcc acggcgtcta
     2161 tacggtgttc ccggtgccgc tggccgaggc ctcggcgttc gacgcggatc tggcccagcg
     2221 gacggcccgg gcgatgtcgc tggaaggcgc agtctcggcc atccaccaga cctatgcacc
     2281 gatggtcgac gtcgcgcgcg accagcgctg ggggcgcgtg gtcgagggcg caggtgagga
     2341 cgtctatctg aacgaggttc tggccgccgc tcgcgtgcgc ggcgtgcagg gcgaaaaggg
     2401 tctggccgac cgcgacgcgc ttctggccac gcccaagcat ttcgtcgcct attccgccgc
     2461 catcggcggg atggaataca acaccacgga catgagcgag gcaacgctgc gcggggtctt
     2521 cctgccgccg ttcaaggctg cgctggacgc cggcgccctg tcggtgatga gcgccttcaa
     2581 cgacctgaac ggggtgccga cctcgggcaa ccaccggatg ctgaccgacg tgctgcgcac
     2641 agagtggggc ttcgaagggt tcgtggtctc ggactacacg tcggaaaacg agctgatcgc
     2701 gcacggcttc gccgaggacg gtcgcgacgc cgcccgcatc gccctgctgg ccggaatgga
     2761 catgtcgatg gtctccggtc tgtttctcga gcacatccct gatctggtcg agaagggcga
     2821 cgtccccctg gcgcgcgtcg atcaggcggt gcgtcgcgtc ctgatgacca aggcggccct
     2881 cggcctgttc gacgatccct atcgcggctg cgacccggag cgagagcgcc tcgtcgtcgg
     2941 ctcgcgtgag caccgcgacc tggcccg
//
LOCUS       DASVTM010000197         4718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_711778,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000197 DASVTM010000000
VERSION     DASVTM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711778"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VF633_05370"
     CDS             complement(<1..473)
                     /locus_tag="VF633_05370"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX8470522.1"
                     /translation="MVVLNKIYTRTGDAGTTGLASGARVSKSDPRVEAYGTVDELNAV
                     IGVARLHSGQNDRIDAMLGRIQNELFDLGADLATPLDPPPKWEALRIVASQVERLESE
                     IDWMNESLKALDSFILPGGSALAAHLHLARTVSRRAERDAIRLVESGAAVTPEAMR"
     gene            complement(483..689)
                     /locus_tag="VF633_05375"
     CDS             complement(483..689)
                     /locus_tag="VF633_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX8470523.1"
                     /translation="MENVFTILILLAVLAVSVTLGFGLYSLYRGGDYARSNSNRLMRL
                     RVALQATAVILLVAGLWWKSTYGS"
     gene            839..1966
                     /locus_tag="VF633_05380"
     CDS             839..1966
                     /locus_tag="VF633_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470524.1"
                     /translation="MTDRSIRNLANLRDSASTARVLNLSSVYNEHNHTDAWSAQPLFR
                     HPALNRSLIIKHRLRSDEVDRFRLRRHVATKIVLPIDAKDLRVGGRYIFVGEIGFETT
                     MLQVFGIGASHPDLHTLMIIDVLPGLDPFLLREQLRRNGVTPAPCYFNLSEADLKAMI
                     AFVSSEITPLVDLSMGPDADLSVDNPVSRLTEKILSNCAGDDLGALGQTLRLKPDEYE
                     EGIFCWKGFLYYKWVLGGVIGDIGAVMEAVRTVKPRGRPNSQQYVALDRSRETVRRRI
                     LASCDSTAAMLRVYDDAFRGLTRNGDPVGFRDFLLDAPHLFAELGERLGAVQHIVSFW
                     NYRMAYGKPKPSPEELFDLLADFEASLAYGEDETRSMSLAA"
     gene            complement(2166..3557)
                     /gene="ahcY"
                     /locus_tag="VF633_05385"
     CDS             complement(2166..3557)
                     /gene="ahcY"
                     /locus_tag="VF633_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8470525.1"
                     /translation="MTDYIVRDISLAPFGNKEIAIAETEMPGLMALRDEFGAQQILKG
                     ARIAGSLHMTIQTAVLIQTLEALGADVRWASCNIFSTQDHAAAAIAAAGTPVFATKGE
                     TLEEYWDYAHKIFEWSDGGYPNLILDDGGDATLLCVLGPKAELDGTLLDNPQNEEEEA
                     LYKVMKRYIAEKPGFYSAIRDAIGGVSEETTTGVHRLYEMAKKGDLPFPAINVNDSVT
                     KSKFDNLYGCRESLVDAIRRGTDVMLSGKVAVVCGYGDVGKGSAASLRQGGARVKVTE
                     VDPICALQAAMEGYEVVTLEDVAGEADIFVTATGNKDVIRVDHMRAMKNNAIVCNIGH
                     FDSEIQVAGLKNFKWDEIKPQVHHVEFPDGKKIILLSEGRLVNLGNATGHPSFVMSAS
                     FTNQTLAQIELWTNAKSYENQVYTLPKHLDEKVAMLHLGKLGAKLTTLSQEQADYISV
                     PTEGPFKPDHYRY"
     gene            3714..>4718
                     /gene="bioB"
                     /locus_tag="VF633_05390"
     CDS             3714..>4718
                     /gene="bioB"
                     /locus_tag="VF633_05390"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX8470526.1"
                     /translation="MADDASPIRNLSRDVTVPRHDWTLAEVEALFSRPFMELVFEAAS
                     VHRRWFDPSEVQKSQLLSIKTGGCAENCGYCSQSASFDTGLRAEKLMDAAAVIAEAMA
                     AKAGGASRFCMGAAWRELKDRDTPKLATMIAGVKALGLETCATLGMLTADQARQLKEA
                     GLDYYNHNLDTGPEYYTQVVTTRTYQDRLDTLAHVREAGMSTCCGGIVGMGEGRRDRA
                     SLLHALATLPEHPDSLPVNALVPVTGTPLGERVLKTGEIDPIEFVRTVAVARLVCPKA
                     MVRLSAGRETMSPEMQALCFLAGANSIFVGGKLLTTANPEQDEDAKLFALLDLRPMQP
                     A"
BASE COUNT          872 a         1504 c         1452 g          890 t
ORIGIN      
        1 cgcatcgcct cgggagttac ggcggcgccg ctctctacca gccggatcgc atcccgctcg
       61 gcccggcgag agaccgtccg cgccagatgc aggtgcgcgg ccaacgccga accacctggc
      121 agaatgaaac tgtccagagc cttcaggctc tcgttcatcc aatcgatctc cgattccaga
      181 cgctcgactt gggaagcgac tattcggagc gcctcccatt tcggaggcgg atcgagaggc
      241 gtagcgaggt ccgcgcccag atcgaacaat tcgttctgaa tccggcccag catggcgtcg
      301 atacggtcgt tttgtcccga atgcagtcgg gcgacaccaa tgacggcgtt caactcatcg
      361 acggtgccgt aagcctcgac gcgcggatcg ctcttggaga cccgtgcccc ggaagccagc
      421 ccagtcgtac cggcgtctcc cgtgcgggtg tagattttgt tcagaacaac caagacctgc
      481 cctcaggagc cgtaagtgga tttccaccac agaccggcca ccaacaagat cacggccgtc
      541 gcctgtagcg ccacccggag ccgcatcagc ctgttggaat tggaccgcgc gtaatcccca
      601 ccccgatata gcgagtacag gccgaacccg agggtcacgg acaccgccag aacggccagg
      661 aggatcagga tggtgaagac gttttccatt atcccagact agtcctccct caaggtcgac
      721 gctagcggtc cgagcacagc gcagtcagga caccgccgtc aacgtttcgg ttaccctgtt
      781 aggcgccgcc ttggtcaacc tttccttctt acggtagagc ttcctcacta ggatgctcat
      841 gaccgatcgt tcgatccgaa atctggccaa tctgcgcgat tcggcctcga cagcccgggt
      901 cctgaatctg tcttcggtct ataacgagca caatcacacg gacgcatgga gcgcccagcc
      961 gctgtttcgc catccggcgc tgaaccggtc cctgatcatc aagcatcgat tgcgtagcga
     1021 tgaagtagac cgcttcaggc tccgaagaca cgtcgcgaca aagattgtcc tgccgatcga
     1081 cgccaaggat ctgcgagttg gtgggcgcta cattttcgtt ggcgaaatcg gcttcgaaac
     1141 gaccatgctg caggtgttcg gtatcggcgc cagtcatccg gacctgcata ccctcatgat
     1201 tatcgatgtc ttgccggggc ttgacccgtt cctgttgcgc gaacaactcc gccgaaacgg
     1261 ggttacgcct gcgccctgct atttcaacct cagcgaagct gacctgaaag ccatgatcgc
     1321 attcgtcagc agcgaaatca cgcccttggt ggatctgtcc atgggaccgg acgctgacct
     1381 ttcggtcgac aacccggtat cgaggctgac tgaaaaaatc ctgtcaaact gtgccggcga
     1441 cgaccttggc gcgctgggcc agaccctgcg attgaaacct gacgagtatg aggagggcat
     1501 cttctgctgg aagggcttcc tctattacaa atgggtgctt ggcggggtaa tcggcgacat
     1561 cggcgccgtc atggaagccg tccgcaccgt taaaccgcgc ggccgtccca acagccagca
     1621 atacgtcgcc ctggaccgaa gtcgcgagac cgtgcgtcgt cgcattctgg cgagttgcga
     1681 cagcaccgcc gccatgctgc gcgtctatga cgacgctttc aggggcttga cccggaatgg
     1741 cgatccggtc ggatttcgcg acttcctgct cgacgctccg cacctgttcg ccgagttggg
     1801 cgagcggctg ggcgccgtgc agcacattgt gagcttctgg aactatcgga tggcctacgg
     1861 aaagccgaag ccgtctccgg aagaactgtt cgacctgctg gccgatttcg aggccagcct
     1921 ggcctacggc gaggacgaga ctcgcagcat gtcactcgcc gcctagacat cgcgtctccg
     1981 gcagcgaaga cgaacggaga cgacaggttg gcgcgaggcg ccgaccagaa gacagcgcga
     2041 gacgacccca gacggctgaa ccccataaac gagaaagggg ccaccggtca cccggtcgcc
     2101 ccctgctgat cgcttgcgcg atctgtcctc cccatccgcc tgcgcgaaca gggaggcaaa
     2161 atcgcctagt agcggtagtg gtccggcttg aacgggcctt cggtcggcac cgagatgtag
     2221 tcggcctgtt cctgcgaaag cgtcgtcagc ttggcgccga gcttgccgag gtgaagcatg
     2281 gcgaccttct cgtcgaggtg cttcggcagg gtgtagacct ggttctcata ggacttggcg
     2341 ttggtccaga gttcgatctg ggccagcgtc tggttggtga acgacgccga catcacgaac
     2401 gaggggtgac ccgtcgcgtt gccgaggttc acgaggcgac cttccgacag caggatgatc
     2461 ttcttgccgt ccgggaactc gacgtggtgg acctgaggct tgatctcgtc ccacttgaag
     2521 ttcttcaggc cagcgacctg aatctccgag tcgaagtgac cgatgttgca gacgatggcg
     2581 ttgttcttca tcgcgcgcat gtggtcgacg cggatgacgt ccttgttgcc ggtcgcggtg
     2641 acgaagatgt cggcttcacc cgcgacgtct tccaacgtga cgacttcata gccttccatc
     2701 gcggcctgca gggcgcagat cggatcgact tcggtgacct tcacgcgagc gccgccttgg
     2761 cgcagcgaag cggccgagcc cttgcccacg tcgccgtagc cgcagaccac ggcgaccttg
     2821 cccgagagca tgacgtcggt gccgcgacgg atcgcgtcga ccagcgattc acggcagccg
     2881 tacaggttgt cgaacttgga cttggtgacg ctgtcgttga cgttgatggc ggggaacggc
     2941 aggtcgccct tcttggccat ttcgtacagg cggtgaacgc ccgttgtggt ttcttccgac
     3001 acgccgccga tagcgtcgcg aatggccgaa tagaagccgg gcttctcggc gatgtagcgc
     3061 ttcatcacct tgtatagcgc ttcttcttct tcgttctgcg ggttgtccag cagcgtaccg
     3121 tcaagctcgg ccttcgggcc aagcacgcag agcagggtcg cgtcgccgcc gtcgtcgagg
     3181 atcaggttcg gatagccgcc gtccgaccat tcaaagatct tgtgggcgta gtcccagtac
     3241 tcttccagcg tctcgccctt ggtggcgaac accggcgtgc cggcggcggc gatggcggcc
     3301 gcagcgtggt cctgggtcga gaagatgttg cacgacgccc agcgaacatc agcgccgagg
     3361 gcttccagcg tctggatcag cacggcggtc tggatcgtca tgtgcagcga accggcgatg
     3421 cgggcgccct tgaggatctg ttgagcgcca aactcgtcgc gcagcgccat caggcccggc
     3481 atttcggtct cggcgatcgc gatttccttg ttgccgaacg gggcaagcga gatgtcgcgg
     3541 acgatgtagt cggtcatgaa ggcgctcctt ggcgtcggct catggccgga tcgcggatgg
     3601 tcggttgccg cgcctatacc ccggcctacg ttacggggca ataaacatat aaggatatcc
     3661 ttatgtaaat acggccgctt ggcgatacac gcgtcggggt ttacgcatcc ggaatggccg
     3721 acgacgcatc tccaatccgc aatctctctc gtgacgtgac cgtcccgcgc cacgactgga
     3781 cgctggccga ggtcgaggcg ctgttctcgc gcccgttcat ggaactggtg ttcgaagccg
     3841 cgagcgtcca ccgccgctgg ttcgatcctt cagaggttca gaagtcccag cttctgtcga
     3901 tcaaaaccgg cggctgcgcc gagaactgcg gctactgcag ccagtcagcc agcttcgaca
     3961 ccgggctcag ggccgagaag ctgatggacg ccgcagctgt catcgccgag gccatggccg
     4021 ccaaggccgg cggggcgtcg cgcttctgca tgggcgcggc atggcgcgag ctgaaagatc
     4081 gcgacacgcc caagctggcg acgatgatcg cgggcgtaaa ggcgctcggt cttgagacct
     4141 gcgccaccct cggcatgctg accgccgatc aggctcgcca gctcaaggag gccgggctgg
     4201 actattataa ccacaacctg gacaccggcc cggagtacta cacccaggtc gtcaccacgc
     4261 gaacctatca ggaccgtctc gacaccctcg cgcacgtgcg tgaagcaggc atgagcacct
     4321 gctgcggcgg gatcgtcggc atgggcgaag gccgtcggga tcgcgccagt ctgctgcatg
     4381 cgctggccac cctgccggaa catcccgaca gcctgccggt caatgcgctg gtgccggtca
     4441 ccggcacgcc gctgggcgag cgcgtgctca agaccggcga gatcgatccc atcgagttcg
     4501 tccgcaccgt cgccgtcgcg cggctggtct gccccaaggc catggttcgc ctgtccgccg
     4561 gtcgcgagac catgagcccg gagatgcagg cgctgtgctt cctcgccggc gcaaactcca
     4621 tcttcgtcgg cggcaagctg ctgaccacgg ccaatcccga acaggacgag gatgcgaaac
     4681 tcttcgccct gctggacctg agacccatgc agccggcc
//
LOCUS       DASVTM010000198         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_711970,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000198 DASVTM010000000
VERSION     DASVTM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711970"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VF633_05395"
     CDS             complement(<1..905)
                     /locus_tag="VF633_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEX8470527.1"
                     /translation="MTTQLLPGCTGVLALADGTILQGIGVGATGSALGEVVFNTAMTG
                     YQEILTDPSYMSQILAFTFPHIGNTGVNVEDIEQIGGGSDTSARGAIFRDLPTDPANW
                     RSESSFDGWMKRRGVVGLSGVDTRALTKIIRDKGAPHAVIAHDPQGKFDLEALVAQAR
                     AWTGLVGLDLAKPASTLQSFEWDEGLWAWPEGHPKTEGRKNVVVIDYGVKRNILRALA
                     STGAKITVVPATTTADEILARNPDGVVLSNGPGDPAATGEYAVPEILKLVDSGKPVFG
                     ICLGHQMLALALGARTVKMDQGHHGA"
     gene            complement(996..>1619)
                     /locus_tag="VF633_05400"
     CDS             complement(996..>1619)
                     /locus_tag="VF633_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8470528.1"
                     /translation="AGSGRYAVVHGLSRSGPDRLDLEDEETIIAATARIAEGPPLTLV
                     FVATGVLHDGEEPERSYRTLNAEHMLRDYRVNTVGPALVAKHFLPLMPKDQRAVFAAL
                     SARVGSIGDNRLGGWHSYRASKAALNMILKNLSIEMARSHPLAIVAGLHPGTVATDLS
                     APFQKGVAPGKLFDAAYSAERLLAVLDGLSPKDSGGVFAWDGSRIPE"
BASE COUNT          259 a          546 c          539 g          275 t
ORIGIN      
        1 gcgccgtggt ggccctgatc catcttcacg gtcctggcgc ccagagccag cgccagcatc
       61 tggtgaccga ggcagatgcc gaacaccggc ttgccgctgt cgaccagctt caggatttca
      121 ggcaccgcgt attcgccggt cgccgccgga tcgcccggac cgttggacag gacgacgccg
      181 tcgggattgc gggccagaat ctcgtcggcc gtggtcgtcg cgggaacgac ggtgatcttc
      241 gcgcccgtcg aggccagcgc ccgcaggatg ttgcgcttca cgccatagtc gatgacgacc
      301 acgttcttgc ggccttcggt cttggggtga ccctccggcc aggcccacag gccctcgtcc
      361 cactcgaacg actgcagcgt cgaggcgggc ttggcgaggt cgaggccgac gaggcctgtc
      421 catgccctgg cctgagccac cagcgcctcg aggtcgaact tgccttgcgg atcatgggcg
      481 atgacggcgt gcggcgcgcc cttgtcgcgg atgatcttgg tcagggcgcg ggtatcgacg
      541 ccggacaggc cgacgacgcc gcgacgcttc atccagccgt cgaagctgga ttcgctgcgc
      601 caattcgctg gatcggtcgg caggtcgcgg aagatcgcgc cccgcgccga ggtgtccgaa
      661 ccgccgccaa tttgttcgat atcctcgacg ttcaccccgg tgttgccgat gtgggggaag
      721 gtgaaggcca ggatctggct catgtacgac gggtcggtca ggatttcctg gtagccggtc
      781 atggcggtgt tgaagacgac ttcgcccagc gccgagccgg tcgcgccgac accgatcccc
      841 tgaaggatgg tgccgtcggc caaggccaga acgccggtgc agccgggaag cagttgagta
      901 gtcatggcgc gtccttacca ccgaatcccg gcgagcaaac ggcggcgtga ctacagaggc
      961 gtatcgaggc ggtcaatctc ggtcattccg gcccctcatt ccgggatgcg cgagccgtcc
     1021 catgcgaaga ccccgccgct gtccttcggt gacaggccgt cgagcaccgc aagcagccgt
     1081 tcggccgaat aggcggcatc gaacagcttg ccgggcgcga cgcccttctg aaacggcgcg
     1141 ctgagatccg tcgcgaccgt gcctggatgg aggcccgcga cgatggccag cggatggctt
     1201 cgcgccatct cgatcgagag gttcttcagg atcatgttca gcgccgcctt ggacgcccga
     1261 tagctgtgcc atccgcccag ccggttgtcg ccgatggagc ccacgcgagc cgacaacgcc
     1321 gcgaacaccg cccgctggtc cttcggcatc agcggcagga aatgcttggc gaccagcgcc
     1381 ggtccgacgg tgttgacgcg gtagtccctc agcatgtgtt ctgcgttcag cgtcctgtag
     1441 gaccgctccg gctcctcgcc gtcgtgcagg acgccggtcg cgacgaagac cagcgtcagc
     1501 ggaggcccct cagcgatccg cgcagttgca gcgatgatgg tctcctcgtc ctcaaggtcc
     1561 agccgatccg gacctgagcg cgacagccca tgtacgacgg cgtatcgccc ggaccccgc
//
LOCUS       DASVTM010000199         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_818840,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000199 DASVTM010000000
VERSION     DASVTM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818840"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1084)
                     /gene="galE"
                     /locus_tag="VF633_05405"
     CDS             complement(62..1084)
                     /gene="galE"
                     /locus_tag="VF633_05405"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX8470529.1"
                     /translation="MLRHVLVTGGAGYIGSHTCKALAQAGHQPVVFDDLSNGHAEAVK
                     WGPLVVGDVRDSAAVASCMREHRIDSVIHFAGLIEVGRSMVEPELFWDQNLNGVAAVL
                     GAMRTCGVDRIVFSSTAAVYGTPPDTAPLNEAAPKQPINPYGDSKLAAERLIAAACRA
                     HGLEGIALRYFNAAGADPDGEIGEAHHPESHLIPLAIEAALGIGQPLTLFGSDFPTPD
                     GTCVRDYIHVCDLAAAHVLALDAPAGPDGFSALNLGNGVGYSVRQVVDAVAATTQCMP
                     PHAIGPRREGDPALLVADPSAARRQLGWSPRFDSLEDIVASAVAWRLKPRFGKRFDIS
                     ADLVAA"
     gene            1222..2946
                     /locus_tag="VF633_05410"
     CDS             1222..2946
                     /locus_tag="VF633_05410"
                     /EC_number="7.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan export ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX8470530.1"
                     /translation="MIVNLYARTLRLLEPERPVVIGLTLANVVLGGLVLLEPLLFGRV
                     IDALTRHASPWRFIAGWAVLGVALIGASAGLSLVADRMAHRRRMSAMNAAFEKAVTLP
                     APFVSETGTGPIIRVMVSGGDALFTLILSFFREQQTAVVSLILLVPLAFWMDPIMASV
                     LLVLAVLYVGASWLVVAKTEVGQAEVEQHHQAVSSRIGDAIANVSVIQAYTRVRGEGA
                     DLRAMTGRLLGAQYPVLSWWALLMVMTRGASTLAMISLFTTGAVLLRAGHVTVGEIVS
                     FIGFANLLIARLDQLSASLARVFVQAPTLSGLFRLMDTDRALRDAPDAEPLIEVRGDI
                     VFDRVTYWFLDTHMGVFDIDLHARAGSTVALVGPSGAGKTTLMSLLQRQREPSLGRIL
                     IDGRDIQRVSLASLRSAIGVVFQDAGLFNRSIADNLGLARPEASRAEIEQAAIAAEAH
                     DFIVAKPGGYDFVIGEGGRLLSGGERQRLAIARAILKNAPILILDEATSALDTVTEDR
                     VKRALTTAAEGRTTFLIAHRLSTVVDADQILMMERGRIIERGTYDQLVEAGGAFARLA
                     RSGLARRE"
BASE COUNT          521 a         1020 c          988 g          548 t
ORIGIN      
        1 aaactgtcgc cgacccggtt gaccgtgcat tcgacgccac cccacatctc aaggtgatcg
       61 gtcatgcggc gaccaggtct gcggagatgt cgaagcgctt gccgaagcgg ggcttgagcc
      121 gccaggcgac ggcggaggcg acgatgtcct cgaggctgtc gaagcggggc gaccagccga
      181 gttgccgacg ggcagctgac gggtcagcga caaggagcgc ggggtctccc tcgcggcgcg
      241 gcccgatggc gtgaggcggc atgcattgtg tggtcgcagc gacggcgtcg accacctgtc
      301 gcacggaata gcccacgccg ttgccgaggt tcaaggcgga gaagccgtcc gggccggccg
      361 gggcgtcgag agcgagaaca tgcgccgccg cgagatcgca cacgtggata tagtcgcgca
      421 cgcaggtccc atccggcgtc gggaaatcag aaccgaacaa ggtcaggggc tggccgattc
      481 ccagcgccgc ctcgatcgcc agcgggatca gatggctttc gggatgatgg gcctccccaa
      541 tctcgccatc cgggtccgcg cccgctgcat tgaaataccg gagcgcgatg ccctccagcc
      601 catgcgcacg gcatgcggca gcgatcagcc gctccgccgc cagcttgctg tcgccgtacg
      661 gattgatggg ctgcttcggc gccgcctcgt tcaagggggc ggtgtccgga ggcgtgccat
      721 agaccgcggc cgtagacgag aacacgatgc gatccacgcc gcacgtgcgc atcgcgccca
      781 gtacggccgc cacgccgttc aggttctggt cccagaacaa ctcgggctcg accatcgacc
      841 ggccaacctc gatcaggccg gcgaaatgga tcacgctgtc gatccggtgc tcacgcatac
      901 agctggcgac ggcggcgctg tcgcgaacgt cgccgaccac cagcggaccc cacttcaccg
      961 cctcggcatg accattgctg agatcgtcga agacgaccgg ctgatggccg gcctgagcca
     1021 acgccttgca ggtatgcgat ccgatatagc cggctccgcc ggtgaccagc acgtgtcgta
     1081 gcattctcga ttcagtcctc gttgcctgtg tgcaaggcgt tgcccgcgcg agtgtttcca
     1141 cctgtcgggt atcagtcaca tcgccgcacc acagaacccg cggcccgcct ggtggttcac
     1201 gcaaagcagc cggagcatgc cgtgatcgtc aacctctacg cccggaccct gcggctgctg
     1261 gaacccgaac gcccggtcgt gatcggtttg acgctcgcga acgtcgtgct cgggggcctg
     1321 gtgctgctcg aaccgctcct gttcggacgg gtaatcgacg cgctcacccg ccacgcctct
     1381 ccctggcgct tcatcgccgg atgggccgtc ctcggtgtcg ccctcatcgg agccagcgca
     1441 ggcctgtccc ttgtagccga tcgtatggcg cacaggcgcc gaatgtcggc catgaacgcg
     1501 gcgttcgaga aggcggtgac actgcccgcc cccttcgtgt ccgaaaccgg cacggggccc
     1561 atcatccgtg tgatggtgtc gggcggcgac gcgttgttca cactgatcct ctcctttttc
     1621 cgcgagcagc agaccgcggt cgtcagtctg atcctcctcg tcccccttgc gttctggatg
     1681 gacccgatca tggcctccgt cctgctggtg cttgcggtgc tctacgtcgg cgcaagctgg
     1741 ctggtcgtcg ccaagaccga ggtgggtcag gcagaggtcg agcaacacca tcaggccgtt
     1801 tcgagccgta ttggcgacgc gatcgccaac gtcagcgtca ttcaggccta tacccgggtg
     1861 cggggagagg gtgcggatct gcgtgccatg accggcaggc ttctcggcgc gcagtatcca
     1921 gttctcagct ggtgggccct gctgatggtg atgacgcgcg gcgcttccac cctggccatg
     1981 ataagcctct ttacgacggg cgccgttctg ctccgcgccg gacatgtcac cgtcggcgag
     2041 atcgtgagct tcataggctt cgcaaacctc ctgatcgccc gcctggatca actgtccgcc
     2101 tctctggccc gtgtcttcgt gcaggcgccc accctgagcg gcctgttccg actaatggat
     2161 acggatcgcg ccttgcggga tgcgccggat gcggaaccgc tcatcgaggt caggggcgac
     2221 atcgtcttcg atcgcgtgac ctactggttt ttggacacgc acatgggcgt gttcgatatc
     2281 gatctgcatg cacgggctgg ctcgacagtc gccctggtcg gtcccagcgg cgcgggaaag
     2341 accacgctca tgagcctgct tcaaagacag cgggaaccgt ccctcggccg catcctgatc
     2401 gatggccgcg acatacagcg cgtcagcctc gcctccttgc ggagcgccat cggcgtggtc
     2461 ttccaggacg ccggcctatt caaccgttca attgcggaca acctgggtct ggcccgtccc
     2521 gaggccagcc gtgcggagat tgagcaggcc gccattgcgg ccgaagcaca cgatttcatc
     2581 gtcgcgaagc cgggcggata tgacttcgtg attggcgagg gcggtcgtct gctttcaggc
     2641 ggagaacgtc agcggctggc gatcgcgcgt gcgatactga aaaacgcacc catcctgatc
     2701 cttgatgagg cgaccagcgc gctggacacc gtcaccgaag atcgggtcaa gcgggcgctg
     2761 acgaccgccg ccgaagggcg aaccaccttc ctcatcgcgc acaggctgtc caccgtcgtg
     2821 gacgccgacc agatcctcat gatggagcga ggccgcatca tcgagcgggg gacctatgat
     2881 caactcgtcg aagccggtgg cgcttttgca agactcgcgc gttccggcct cgccagacga
     2941 gaatgaacga agggtggccg accccggata ggccgcattc atgtcggacg ttgtaactac
     3001 ccattttgtg gcgcactgcg tcgaaccatt gcccggagat cgattgtgcc agaaccgagc
     3061 ctgcgagacc gtcgcat
//
LOCUS       DASVTM010000200         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1028525,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000200 DASVTM010000000
VERSION     DASVTM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028525"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF633_05415"
     CDS             complement(<1..416)
                     /locus_tag="VF633_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX8470531.1"
                     /translation="MLDFDRYAFLTGATAFLIAFAAAGVFTIAFKLIYQWVTPYHEAT
                     LIRDGNTAAAISLGGALIGYVLPLASALSHTVTLREFAAWALLAAVIQILAFVVVSRL
                     VYRKMAERIEAGEMSAAVYLASISICIGLLNAACMTA"
     gene            complement(487..1149)
                     /locus_tag="VF633_05420"
     CDS             complement(487..1149)
                     /locus_tag="VF633_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HEX8470532.1"
                     /translation="MFKRLFGGQEAPAAAPALAVVRNITIGRTVALDPLAWRRLGDQT
                     KFTLDRDVLDITAQGVVTLDTGQHVHRFYTDDHIMLQAMSDDPQGLESYDFSLFMPWT
                     SAYPPGEQQRRIWKDRLSAPIFHGAAESLPDYPRFWFTEADGIQPPVTLWETIYDDRA
                     ARTPYSKIFQTCMLYARDLPDGRELMMALEMQPEDAQGKPGDLSHEIMIGIPLEMAEF
                     RA"
     gene            1537..2001
                     /locus_tag="VF633_05425"
     CDS             1537..2001
                     /locus_tag="VF633_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HEX8470533.1"
                     /translation="MDAVIETPWTVPAIKTALDDSTLIRDGEMTLRVLEGAEPVLLAT
                     MHEYGDLPIYLSVGGAQIVCSVLLWPVSEQKDAARFNEFLLKAQRVVPLSNFAITTVG
                     GEDVYELMGELSCKTTLQTILIELRTLAENAIDATALRETFDAPLNAPVLQA"
     gene            2024..>2382
                     /locus_tag="VF633_05430"
     CDS             2024..>2382
                     /locus_tag="VF633_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX8470534.1"
                     /translation="MSMLSKLSALFRGTAHEAGQSVVDANALKILDQEIRDADNSQGK
                     ARDDLAGLVARRRMAENELQSFGEQISKYENSARTALGQGKVDLAREVAGRIAELESE
                     IGTRGPQIENMKQAEARL"
BASE COUNT          450 a          735 c          790 g          407 t
ORIGIN      
        1 gccgtcatac aggccgcgtt gagcagcccg atgcagatgg agatggaggc cagatagacg
       61 gcggcggaca tttcgcccgc ctcaatgcgt tcggccatct tgcgatagac cagacggctg
      121 acgaccacga aggccaggat ctgaatcacg gcggccagca gggcccaggc ggcgaactcg
      181 cgcagggtca cggtgtgcga cagggcggag gccagcggca ggacatagcc gatcagggcc
      241 ccgcccagcg agatggcggc ggcggtgttg ccgtcgcgga tcagggtcgc ctcgtgatag
      301 ggcgtgaccc actggtagat cagcttgaag gcgatggtga agacgcccgc cgcggcgaag
      361 gcgatcagga aggcggtcgc cccggtcagg aaagcgtagc gatcgaagtc gagcattctt
      421 gtctccccat ttgcgccggc ggcgcgaaag tctggtgcgc cctgcggcgc ttaagtcagt
      481 gtgccgtcag gcgcggaact cggccatctc cagagggatg ccgatcatga tttcgtggct
      541 gaggtcgccg ggtttccctt gggcgtcctc gggctgcatc tccagcgcca tcatcaactc
      601 gcgaccgtcg ggaaggtccc gggcgtagag catgcaggtc tggaagattt tcgaatacgg
      661 cgtgcgggcg gcgcgatcgt catagatcgt ctcccacagg gtcaccggag gctggatccc
      721 gtccgcctcg gtgaaccaga agcgcggata gtcgggcagg ctttcggctg cgccgtggaa
      781 aatcggcgcg gacaggcgat ccttccagat gcgacgctgc tgctcgcctg gcggataggc
      841 cgaggtccat ggcatgaaga ggctgaagtc gtagctttcc agcccctgcg gatcgtcgct
      901 catggcctga agcatgatgt ggtcgtcggt atagaaccgg tggacatgct ggcccgtgtc
      961 gagcgtgacg acgccctgcg ccgtgatgtc gaggacatcg cggtcgagcg tgaatttagt
     1021 ctggtcgccc agccggcgcc aggccagcgg gtcgagggcg acagtccggc cgatggtgat
     1081 gttgcgcacc actgccagtg ccggggccgc agccggtgct tcctgtccgc cgaagagacg
     1141 cttgaacatg aactgtcctt accctcgatc cgttgaagcg gcgaggggtc gtcttacacg
     1201 atccggttgt tcgtgggcca tgaggcaaaa tcatccgatg cgaccctctt gtcaagcgtt
     1261 cctgttacgc gttacatgta acgaatgacg agcggagacg acagttcatc gcctgatgcc
     1321 tcgccagccg ccgcaaccga tcgcatccgg gcgtggcgca aggcgaggcg cgaggcgggc
     1381 atggtcaagc tggagttctg ggttccccag acgacgcgtg acgatgtgaa ggcggctgtg
     1441 cgcgcaatcg tcacggactc gacccgctca cctgctattg cgacccgacg ccgatcttct
     1501 caaatccaag acctcacccc tggagccgac catcaaatgg acgccgtgat cgaaaccccc
     1561 tggaccgtgc ccgccatcaa gaccgcgctg gacgactcga ccctgatacg cgacggcgaa
     1621 atgacgctgc gggtgctgga aggcgccgag ccggtgctgc tggcgaccat gcatgaatat
     1681 ggcgacctgc cgatctatct gtcggtcggc ggcgcccaga tcgtctgttc ggtgctgctg
     1741 tggcccgtgt ccgaacagaa ggacgcggcc cggttcaacg agttcctgct caaggcccag
     1801 cgggtcgtgc cgctgtcgaa cttcgccatc accacggtgg gcggcgaaga tgtctatgag
     1861 ttgatgggcg aactgtcgtg caagacgacg cttcagacca tattgatcga attgcgcacc
     1921 ctggccgaga acgcgatcga cgccaccgcc ttgcgcgaaa ccttcgacgc tcccctcaac
     1981 gctcctgtgc tgcaagcctg attatcggaa ccggaaaacg accatgtcca tgctttccaa
     2041 actgtccgcc ctgttccgcg gcacggccca tgaggccggc cagtcggtgg tcgatgccaa
     2101 tgcgctgaag atcctcgatc aggaaatccg cgacgccgac aactcgcagg gcaaggcccg
     2161 cgacgacctc gccggtctgg tcgcgcgccg ccgtatggcc gagaacgaac tgcagagctt
     2221 cggcgagcag atctctaaat acgaaaactc ggcccgcacc gccctggggc agggcaaggt
     2281 cgatctggcg cgcgaagtcg ccgggcggat cgccgagctc gagagcgaga tcggcacgcg
     2341 cggccctcag atcgagaata tgaaacaggc cgaggcgcgg ct
//
LOCUS       DASVTM010000201         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_499459,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000201 DASVTM010000000
VERSION     DASVTM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499459"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VF633_05435"
     CDS             complement(<1..175)
                     /locus_tag="VF633_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8470535.1"
                     /translation="MARVSIQAVSRVFAGSRRQPETVALQRSSLEVADNDFITVLGPS
                     GCGKSTLLRIVAGL"
     gene            complement(213..989)
                     /locus_tag="VF633_05440"
     CDS             complement(213..989)
                     /locus_tag="VF633_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8470536.1"
                     /translation="MRPLQPVAPRTRIALGVSFFILFTAAWAFATFGGFVSRTFLADP
                     LTMLRDGWDLLAVHGFLADIGITIARVVGGFVMAAVLAVPLGLLMGAYKPIEAFFEPF
                     VSFARYLPASAFIPLLILWAGIGELQKLLVIFIGTFFQLVLMVAVVVGNTRRDLVEAA
                     YTLGASDRSLVNRVLLPNSAPEIAEMLRLVLGWAWTYVIVAELIGASSGIGHMITDSQ
                     SLLATGQIIFGIIVIGLIGLVTDFVFKFGNRILFPWAALR"
     gene            complement(1012..>1779)
                     /locus_tag="VF633_05445"
     CDS             complement(1012..>1779)
                     /locus_tag="VF633_05445"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8470537.1"
                     /translation="QASRHLTLASGDIQCAATTVETWVVWNANGVPAKQIFQLDKSYG
                     ADGMVVRGGVGSIKDLKGKTVAASAPGTAPYFTLAWFLKKNGLSVKDVRVVNMEPNPA
                     AQAFVAGQSGIDAALTYEPYLSLVRDKPEAGKIIATTLDYPMIMDTVGCSPKFIQDNP
                     KAVKALADSYFEALDYIAANKDKAYEIMGADVKQSGEQFGRSAAFLRWQGRDENRKFF
                     SGELQAFTKEAADLLLEAGIIKAIPDLGAIIDDSFVK"
BASE COUNT          326 a          596 c          579 g          278 t
ORIGIN      
        1 cgagccccgc cacgatccgc aacagggtcg acttgccgca gcccgacggc cccagcaccg
       61 tgatgaagtc gttgtccgcc acctccagcg acgaacgctg cagggccacc gtctcgggtt
      121 ggcgtcgcga gcccgcaaaa acgcgggaca ccgcctgaat ggagacccgc gccatcagcc
      181 gccgcccgcg atctgagttc cccgcccgct gttcatcgca acgccgccca agggaacagg
      241 atccggttgc cgaacttgaa gacgaagtcc gtcaccagac cgatcaggcc gatcacgatg
      301 atgccgaaga tgatctggcc ggtcgcgagc agcgactggc tgtcggtgat catgtggccg
      361 atgccggaag acgcgccgat cagctcggcc acgatgacat aggtccatgc ccagccgagc
      421 acgaggcgca gcatctcggc gatttccggc gccgagttcg gcagcagcac ccggttgacc
      481 agcgaccggt cgctcgcccc gagggtgtag gcagcctcga ccaggtcgcg gcgcgtgttg
      541 ccgaccacga ccgccaccat cagcaccagc tgaaagaagg tgccgatgaa gatcacgaga
      601 agcttctgca gctcgccgat gccggcccac aggatcagca aggggatgaa agcggacgca
      661 gggaggtagc gggcaaagga tacgaagggc tcgaagaacg cctcgatcgg cttgtaggcc
      721 cccatcagca gccccaacgg cactgccagc acggcggcca tgacgaagcc gccgaccacc
      781 cgggcgatgg tgatgccgat gtccgcgagg aagccgtgca ccgcaaggag gtcccagccg
      841 tcccgcaaca tggtcagcgg atcggcgagg aacgtccggc tcacgaaccc gccgaacgtt
      901 gcgaacgccc aggcggcggt gaacaggatg aagaaggaca ccccgagcgc gatgcgcgtc
      961 cggggggcga ccggctgcag cggcctcacg gacgagtccg gaaggccgtc gtcacttcac
     1021 gaacgagtcg tcgatgatgg cgccgaggtc cggaatggcc ttgatgatgc ccgcttccag
     1081 cagcaggtcg gcggcttcct tcgtgaaggc ctgcagttca cccgaaaaga acttccggtt
     1141 ctcgtcgcgc ccctgccagc gcaggaacgc ggccgacctg ccgaactgtt cgccgctctg
     1201 cttgacgtcc gcgcccatga tctcgtaggc cttgtccttg ttggccgcga tgtagtcgag
     1261 tgcctcgaag tagctgtccg ccagcgcctt gaccgccttc gggttgtcct ggatgaattt
     1321 cgggctgcag ccgacggtgt ccatgatcat cgggtagtcc agcgtcgtcg cgatgatctt
     1381 gcccgcttcc ggcttgtccc ggaccagcga gaggtacggc tcataagtga gggccgcgtc
     1441 gatgccgctc tgcccagcca cgaaagcctg tgccgccggg ttcggctcca tgttcaccac
     1501 tctgacgtct ttcaccgaca ggccgttctt cttcaggaac caggccaggg tgaagtaggg
     1561 ggcggtgccg ggagcggagg ccgcgacggt cttgcccttg aggtccttga tcgagcccac
     1621 cccgccgcgc acgaccatgc cgtcggcgcc gtaggacttg tcgagttgga agatctgctt
     1681 cgccggcacg ccgttggcgt tccacacgac ccaggtctcg accgtggtcg cggcgcactg
     1741 gatgtcgccc gacgccaggg tgagatggcg gctcgcctg
//
LOCUS       DASVTM010000202         4710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_657024,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000202 DASVTM010000000
VERSION     DASVTM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657024"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF633_05450"
     CDS             complement(<1..206)
                     /locus_tag="VF633_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470538.1"
                     /translation="MSAFEFFFSFYGLVLGLSVAALATGAARAVKHRKTVRIGWLTTM
                     LAVFVALDIATFWDAAWVNFREVP"
     gene            complement(272..904)
                     /locus_tag="VF633_05455"
     CDS             complement(272..904)
                     /locus_tag="VF633_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470539.1"
                     /translation="MSAFEFFFSFYGLLLGLSVAELVGGFARVLHQRRAVRFGWLTPL
                     LAVFVAIDIATFWNQAWFIFRFAPFNMALLVVGLAVAAVFYVAASVTFPQREAEASSD
                     LDDHFWAHRRTVFLCVLLANLIIMAVFFGVTAISGEMTTLKPGPIFWIGLCLFTGGTS
                     LAAFAPSRKLVMTALIVLVAYHGFSVARSTHALIEGGGWSLKAPKAAGGR"
     gene            complement(1056..1397)
                     /locus_tag="VF633_05460"
     CDS             complement(1056..1397)
                     /locus_tag="VF633_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470540.1"
                     /translation="MKAFLIAILVLSSAPVVVPTYADAQVLAGRGAARAAERRARPAL
                     SVREEDRLYATQDQIATLEEQIATLQAAGQTAGGLTPEQTTQIAAHRAHLAEARTVVE
                     RLEAKRARASN"
     gene            complement(1533..2018)
                     /locus_tag="VF633_05465"
     CDS             complement(1533..2018)
                     /locus_tag="VF633_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEX8470541.1"
                     /translation="MSEPVPYPPEAHDFDFIHGQWTVANRRLKTRGVGADDWDVFAST
                     AFCEPRLGGLANVEEVSCPERGWTGMAVRAFDREQGQWSVWWISSLTGQLQTPVTGRF
                     GSDGCVLEGEDLDGDWPIRARYIWSDIKPDSARWTQAFSYDDGSTWETNWVMDFARET
                     P"
     gene            2102..3076
                     /locus_tag="VF633_05470"
     CDS             2102..3076
                     /locus_tag="VF633_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8470542.1"
                     /translation="MRASRLLSILLILQTRGRTTASALAAEFEVSVRTVYRDIDQLSA
                     AGVPVYADRGREGGFQLMEGYRTRLTGLTDAEAETLFLGGLAGPAAEMGFSQALMTMQ
                     LKLLAALPPERQAAAERMAARFHLDPAGWYQADTGAARLPELAQAVWAQHLIQIRYES
                     WKGPVERTLEPLGLILKGGVWYIAARPAGKTKAEARTYRVSNILALEVSQEAFSRPED
                     FDLAGWWTATSNRFEAGLFIGEAGLKLTGEGLRRLAQLGPRYATIAARTPVPTPGAWV
                     GVTVPIESIAHAARELLRLGADAEVLAPPNLRTAMAETTQRMAALYRD"
     gene            complement(3083..4612)
                     /locus_tag="VF633_05475"
     CDS             complement(3083..4612)
                     /locus_tag="VF633_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX8470543.1"
                     /translation="MAYRSLREFMARLEAAGELVRVKEPVSTVLEMTEIQTRLLRNGG
                     PAVLFEKPVMPDGSISPIPALANLFGTVKRVAMGVTMDKKTRTTAAELREVGELLAFL
                     RNPTPPRGLGDAMEMLPLMQTVMSMRPKVVKTAPVQSVVLKGDQIDLTSLPVQGCWPG
                     EPAPLITWGLVVTKGPSDDREDDFNLGIYRMQVLGKDKAIMRWLAHRGGAQHYARHKK
                     AGKREPLPCAVVLGADPGTILAAVTPVPDTLSEYQFAGLLRGAKAELVACKTVPLMVP
                     ANAEIVLEGHVLLDEHEDEGPYGDHTGYYNSVEKFPVFKVSAITMRKDPIYLTTFTGR
                     PPDEPSVLGEALNEVFIPLLRAQFPEITDFWLPPEGCSYRIAVVSMKKAYPGHAKRVM
                     LGVWSYLRQFMYTKWVIVVDDDIDARDWKQVMWAISTKMDPARDITVIENTPIDYLDF
                     ASPESGLGSKIGLDATDKWPPETHREWGEEIRMDEAVVEKVSDMWERLGLPGDGKPIW
                     K"
BASE COUNT          887 a         1507 c         1537 g          779 t
ORIGIN      
        1 tacggcacct cgcggaaatt gacccatgca gcatcccaga aggtcgcgat gtccagcgcc
       61 acgaacacgg ccagcatcgt cgtcagccag ccgatgcgaa ctgtcttgcg atgcttgacc
      121 gctcgggccg cgcccgtggc aagcgcggct accgaaaggc cgaggaccaa gccgtagaag
      181 ctgaagaaga actcgaacgc gctcaaatcg attccccgaa agccaagact ggcgacgcta
      241 gagagtttga cgaaccgcga ctaccggaag gttaccgtcc gcccgccgct ttcggagcct
      301 tgagcgacca gcctccgcct tcgatcaggg catgggtcga gcgcgcgacc gagaacccgt
      361 gataggccac cagtacgatc agggcggtca tcacgagctt gcgcgacggt gcgaaggcgg
      421 ccagcgaagt cccgccggtg aacagacaca ggccgatcca gaagatcggg cccggcttca
      481 gcgtcgtcat ttccccgctt atcgccgtga cgccgaagaa gaccgccatg atgatcaggt
      541 tggcgagcag tacgcacaga aagaccgtgc gccggtgggc ccagaaatga tcgtccagat
      601 cgctcgaggc ttcagcctcg cgctggggaa aggtcacgct cgccgcgacg tagaagacgg
      661 cggcgaccgc cagtccaacc accagcagcg ccatgttgaa cggggcgaag cggaagataa
      721 accacgcctg attccagaag gtggcgatgt cgatggccac gaacaccgcc aacaacggcg
      781 tcagccaacc gaaccgaacg gcccgtctct gatgcagaac acgcgcaaat ccaccaacca
      841 gctcggcgac ggatagcccg agcagcaaac cgtagaaact gaagaagaat tcgaacgcgc
      901 tcatgcccac cccgtctaaa caagcgtcac cctaaagggc gatccggggt tcgtcacccg
      961 gtgacgcctt caaacgaggg caagccgttc aaagaacagc tatcgaccca aacaagccgg
     1021 ccgttgtcaa ccagaagcgg aagaagcctg cgacctcagt tggaggcgcg cgcccgcttg
     1081 gcttcgagcc gctcgacgac cgtacgagcc tcggccaagt gtgcgcggtg agcggcgatc
     1141 tgcgtcgtct gttccggcgt caggccgcct gcggtctgac ctgccgcttg cagcgtcgcg
     1201 atctgctctt ccagcgtcgc gatctgatcc tgagtggcgt acagccggtc ctcttcacgc
     1261 acgctgaggg cagggcgggc gcgccgttcg gctgcacgcg cagcaccacg accggcaaga
     1321 acctgagcat cagcgtaggt gggaacgacc acgggggcag acgacaggac gaggatggcg
     1381 atcagaaagg ctttcataag aagctccggc gacaaacaaa ttgggacgat attacgacat
     1441 taatatttcg tcacgttcta aatggtgcgg tgcagcgggt aagtacggcc tgcgatcgcg
     1501 ccgggaagcg ccaacatcgc aacacagcga gctcacggag tctcccgcgc gaaatccatg
     1561 acccaatttg tctcccatgt tgagccgtcg tcataggaaa aggcctgggt ccaccgagcg
     1621 ctgtctggct tgatgtcaga ccagatatac cgggcgcgga ttggccagtc gccgtcgagg
     1681 tcctcgccct ccagcacgca gccgtcggaa ccgaagcgtc ccgtgacggg cgtctggagt
     1741 tggcccgtca ggctgctgat ccaccatact gaccattggc cttgctcccg gtcgaacgcc
     1801 cgcaccgcca tgcccgtcca gccccgctcc ggacaggaga cctcttcgac gttagccaga
     1861 ccgcccagac gcggctcaca gaaggcggtg gaggcgaaga catcccagtc atcggccccg
     1921 acgccgcggg tcttcagccg ccgattggcg accgtccact ggccatggat aaagtcgaag
     1981 tcgtgggctt caggggggta ggggacaggt tcggacatct tgaactcctc gtcggatgtg
     2041 aacgaggaat agatcgagta acctgtcacc tgatgtcagg attaccggct atgctcgcga
     2101 aatgcgcgct agccggcttc tttcgatcct gctgatcctc cagacccggg ggcgaaccac
     2161 cgcgagcgct ctggctgcgg agttcgaagt ctcggtgcgg acggtctacc gcgacataga
     2221 tcagctgagt gccgctggcg tgccggttta tgccgaccgt gggcgcgaag gggggttcca
     2281 gctcatggaa ggataccgca cgcggctaac ggggctcacc gatgccgagg cggagacgtt
     2341 gtttctcggc ggattggcgg gcccagcggc ggagatgggg ttctctcagg cgctgatgac
     2401 catgcagctg aagctgctcg ccgccctgcc gcctgagcgg caggcggctg cagagcgcat
     2461 ggcggcgcgg tttcatctcg accctgcggg ctggtaccag gcggacactg gggccgcacg
     2521 actgccggaa ctggcgcagg cggtctgggc gcagcatctg atccagatcc gctacgaaag
     2581 ctggaaaggg ccggtggagc gaacgctgga gccgctgggg ctgatcctga aaggcggggt
     2641 ctggtacatc gccgcgcgac ctgcaggaaa gaccaaagcc gaggcgcgga cctatcgggt
     2701 gtcgaatatt ctggcgttgg aggtgagcca agaggcgttc tcccggcccg aagacttcga
     2761 cttggcgggc tggtggaccg cgacctcgaa ccggttcgag gctgggctgt tcatcggtga
     2821 agccgggttg aagctgacag gcgaggggtt gaggcggctc gcgcagctgg ggccccgcta
     2881 tgcgaccatc gccgctcgaa cgccggttcc gacaccgggt gcatgggtcg gggtgaccgt
     2941 gccgatcgag tcgatcgccc acgccgcccg tgaacttctg cgactgggag ccgacgcgga
     3001 ggttctcgca ccgccgaacc tcaggaccgc gatggcggag acgacccaga gaatggcggc
     3061 gttgtatcgc gactgacccg acctatttcc agattggttt cccgtccccc ggcagcccca
     3121 gccgctccca catgtcgctg accttctcga ccactgcctc gtccatgcga atctcttcgc
     3181 cccattcgcg gtgggtctcg ggcggccatt tgtcggtggc gtcgaggccg atcttggagc
     3241 cgagaccgct ctcgggggag gcgaagtcga ggtagtcgat cggggtgttc tcgatcaccg
     3301 tgatgtcgcg cgcgggatcc atcttcgtcg agatggccca catgacctgt ttccagtcgc
     3361 gggcgtcgat gtcgtcatcc acgacgatga cccacttcgt gtacatgaac tggcgcagat
     3421 agctccagac gcccagcatc acccgcttgg cgtggcccgg ataggccttc ttcatcgaca
     3481 ccacggcgat ccggtaggaa cagccttcgg gcggcagcca gaagtcggtg atctccggaa
     3541 actgcgctcg cagcagcgga atgaacacct cgttcaaggc ctcgcccagc accgagggct
     3601 cgtccggcgg gcgaccggta aaggtggtca gatagatcgg gtccttgcgc atggtgatgg
     3661 cgctgacctt gaacaccggg aacttttcga ccgagttgta gtagccggtg tggtcgccat
     3721 aggggccttc gtcctcatgc tcgtcgagca ggacatggcc ttccagaacg atctcggcgt
     3781 tggcgggcac catcagcggc accgtcttgc aggcgaccag ctcggccttg gcgccacgca
     3841 gcaggccggc gaactggtat tccgacaggg tgtccggcac cggcgtcacc gccgccaaga
     3901 tggtgcccgg atcggcgccc agcaccacgg cgcagggcag gggctcgcgt ttcccggcct
     3961 tcttgtgccg cgcatagtgc tgggcgccac cacgatgggc cagccagcgc atgatcgcct
     4021 tgtccttgcc cagcacctgc atacggtaga tgccgagatt gaagtcgtcc tcgcggtcgt
     4081 cggacggccc cttggtgacc accagccccc aggtgatcag cggggccggc tcgccgggcc
     4141 agcagccttg caccggcagg gaggtcaggt cgatctggtc gcccttgagc accaccgact
     4201 gcaccggcgc ggttttcacc accttgggcc gcatcgacat gacggtctgc atcagcggca
     4261 gcatctccat cgcgtcgccc agaccgcgcg gcggcgtggg attgcgcagg aacgccagca
     4321 gttcgccgac ctcgcgcagt tcggcggcgg tggtgcgggt tttcttgtcc atggtcacgc
     4381 ccatggccac gcgtttgacg gtgccgaaca ggttggccag cgccgggatc gggctgatcg
     4441 aaccgtcggg catcaccggc ttttcgaaca ggacagcggg gccgccgttc cgcagcagcc
     4501 gggtctggat ctcggtcatc tccagcacgg tggagacggg ctccttcacg cgcacgagtt
     4561 cgccggcggc ttcgagccgc gccatgaatt cgcgaaggga acgataggcc atcggccgca
     4621 ggcttagggc gcgcccgccg gagtgtcacc cttgagcagg cgcttgggcg cccggcggcg
     4681 ccagctgcgc tccacgtaag gccgcagtcg
//
LOCUS       DASVTM010000203         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_657113,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000203 DASVTM010000000
VERSION     DASVTM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657113"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1117
                     /locus_tag="VF633_05480"
     CDS             260..1117
                     /locus_tag="VF633_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX8470544.1"
                     /translation="MRLPLPASKIRTGLSLLAIVAATLTISACNGGKNRPKLAYEERP
                     VELLYNTGYERLQSHRWSDAVDYFQEVERQHPYSEWSRRAILMQIYAFYQNGSYADSI
                     AASDRFIQLFPGSPSAAYAFYMKATCNFEQIVDVGRDQGYAQNALDGLKDVVRRYPRS
                     AYATDATVKIDMVNDQLAGKEMNIGRYYQRANQPLAAIGRYKAVIDSEAFQRTSHTPE
                     ALYRLVEVYLSLGLKDEATRNGAVLGFNYPGSPWYAEAYALLNEQGETPGTAPTGQRE
                     GWLRRIIPG"
     gene            1215..>2485
                     /locus_tag="VF633_05485"
     CDS             1215..>2485
                     /locus_tag="VF633_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8470545.1"
                     /translation="MLTSLAIRDVVLVDVLDLDVDTGLTVLTGETGAGKSIILDALGL
                     ALGGRGDAGLVRAGAKQASATAVFTVPDDADLIALIAERGFDVVVGEDLILRRVLSAD
                     GRSRAYINDQPAGVAALREIGSRLVEVHGQHETVGLLDWRTHRSLLDAYGGLSPQLSA
                     VSAAAETLKAAEARLAELRAAAADAASRAEEIALNLADLDALDPGDDEETELAGERAI
                     LGAAEKAMGDLADARTQLGGDKLSQKLSAALRAVEHARQRATQAGAEADHPVIARLAA
                     AAEAIDRTIVEAVEAIAEVDAAAAAFDFEPGRLDKTEERLFALRAAARKLNTTVSALP
                     KLRVSLREQLRLIEDGAEALTAAQRETTVARENYDIAATFLTSAREAAGDRLTAAVMG
                     ELGPLKLDRARFRVAFEPVGEGRRGPLGVETV"
BASE COUNT          425 a          802 c          816 g          442 t
ORIGIN      
        1 gcctgggcga gttgccggtt cgagttgcct gcaagcagct tcatcgccgg tcatcctttc
       61 gccgtcatcg gaccgccaat gacgcgtgtt catttcccgc gctcctttac cagcgcgccg
      121 gacgggagca agcgatgagc ggtcgatttc agccttaacg gtagatttgg cacgcattgg
      181 cttgggaccg gtggctggtg tagaggttcg cggtcatcat cggggcgacg cgcggacgcg
      241 ccgtcccttg cggcgtgtca tgaggttacc gttgcctgct tcgaagatcc gtaccggcct
      301 gagccttctg gccatcgtcg ccgcgaccct gaccatttcc gcctgcaatg gcggcaagaa
      361 ccggccgaag ctggcctatg aagagcgccc ggtggagctg ctctacaaca ccggctatga
      421 gcggctgcag agccaccgct ggtcggacgc ggtcgactac ttccaggaag tcgagcgcca
      481 gcatccgtat tcggaatggt cgcgtcgcgc gatcctgatg cagatctacg ccttttatca
      541 gaacggcagc tatgccgact cgatcgctgc gtcggatcgc ttcatccagc tgtttccggg
      601 tagcccctcg gccgcctacg ccttctacat gaaggcgacc tgcaactttg aacagatcgt
      661 cgatgtgggc cgcgaccagg ggtatgccca gaacgcgctg gacgggctga aggatgtggt
      721 gcgtcgctat ccgcgctcag cgtacgccac cgacgccacg gtcaagatcg acatggtcaa
      781 cgatcagctg gccggcaagg aaatgaatat cggtcgctat tatcagcggg ccaaccagcc
      841 actggctgcg atcggacgct ataaggccgt gatcgacagc gaagcctttc agcgcacctc
      901 gcacacgccg gaagcgctct atcggctggt ggaagtctat ctttcgctgg gcctgaagga
      961 cgaagcgacc cgcaacggcg ccgtgctggg cttcaactat ccgggcagcc cttggtacgc
     1021 cgaggcttac gccctgttga acgagcaagg tgagacacca ggaactgccc cgaccggtca
     1081 gcgcgaaggc tggttgcgcc ggatcattcc gggctgaccg cgaggtcatt cgcgataggg
     1141 tctgttctgg gttggttcct gacggcatag agtgaaatcg atgtcgaatc acgtcctgcc
     1201 atgctgattg ccccatgctg acgtctctgg cgatccgcga cgtcgtgctg gtcgatgtgc
     1261 tcgacctcga cgtcgatacc ggcctgacgg tgctgaccgg cgaaactgga gcgggcaagt
     1321 cgatcattct cgacgcgctg ggacttgcgc tcggcgggcg aggggatgcc ggtctggtcc
     1381 gcgcgggcgc caaacaggcc agcgccaccg ccgtcttcac cgttcctgac gatgccgatc
     1441 tgattgcgtt gatcgccgag cgcggattcg acgtcgtggt gggcgaggac ctgatcctgc
     1501 gtcgggttct gtcggcggac ggccgcagtc gggcttatat caacgaccag cccgccgggg
     1561 tcgccgcgtt gcgcgagatc ggctcgcggc tggtcgaggt ccatggtcag catgaaaccg
     1621 tcggtctgct ggactggcgc acccatcgtt cgctgctcga cgcctatggt ggcctatcgc
     1681 cccaactgtc cgccgtgagc gccgccgcag agactctcaa ggccgccgag gcgcgactgg
     1741 ccgaattgcg ggcggccgcc gccgacgcgg catcccgggc ggaagagata gcactcaacc
     1801 ttgccgacct cgatgcgctc gatcccggcg acgacgagga gacagaactg gccggagagc
     1861 gggccattct gggcgccgcc gaaaaggcga tgggtgatct ggctgacgcc cgcacccagc
     1921 tcggtggcga caagctgagc cagaaactgt cagcagcact gcgcgccgtc gaacatgcac
     1981 gccagcgcgc gacgcaggcc ggcgccgagg ccgatcatcc ggtcatcgcc aggctcgccg
     2041 ccgccgccga ggccatcgat cggacgatcg tcgaggctgt cgaagccatt gccgaagtcg
     2101 acgccgccgc cgcagcgttc gacttcgaac ccggtcgact ggacaagacc gaggagcggc
     2161 tgttcgccct gcgcgccgct gcccgcaagc tgaacaccac cgtgtcggcc ctgccgaaac
     2221 tgcgtgtctc actccgtgag cagttgcgct tgatcgagga cggggccgaa gccctgaccg
     2281 ccgcccagcg cgagacgacc gtggcgcgtg agaactacga catcgccgcg accttcctga
     2341 cttccgcccg agaagctgcg ggcgatcggc tgaccgccgc cgtcatgggt gagttgggtc
     2401 cgctcaagct ggaccgtgcc cgtttccgcg tggctttcga gccagtcggc gagggcagga
     2461 gaggcccctt gggcgtggag accgt
//
LOCUS       DASVTM010000204         6496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_869129,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000204 DASVTM010000000
VERSION     DASVTM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869129"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VF633_05490"
     CDS             complement(<1..282)
                     /locus_tag="VF633_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX8470546.1"
                     /translation="MSSPLHRAMTATVGALGHPASLVVLLICAVVWQRVEPHSFDLHA
                     VATLVAVAMTLIIQRGQNRDTAALQAKLDELILATEGARNELAGLDQESV"
     gene            complement(410..787)
                     /locus_tag="VF633_05495"
     CDS             complement(410..787)
                     /locus_tag="VF633_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8470547.1"
                     /translation="MAVFNKDIIKLTETNKNWQKEAYYDELVQIVMMSIPAGEDIGEE
                     THDADQTTFVVSGEGQVVVDGSRTKVTANHLVVIPRGAKHNIINKGEDDLKIFTVYAP
                     PAEDEGVSFKTKEEAEAAEEDED"
     gene            complement(865..1491)
                     /locus_tag="VF633_05500"
     CDS             complement(865..1491)
                     /locus_tag="VF633_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX8470548.1"
                     /translation="MTQFAVPAFAPVADLGDLLPQPVRSDELLNRLATRRSAPAQTLT
                     APGPDAAEVAEIIRLGARTPDHGKLFPWRFVVMGPQTRATLGESLSVLAVRKELGVKA
                     QAVLAKLTAAPVSILVISTARPGPKPVWEQELSSGAVCMNIEHAANALGYASCWITDW
                     YSYDPDALTLLGVKAGEKVAGFIHIGATVEPPLERVRPDLSTLTTTLP"
     gene            1537..2190
                     /locus_tag="VF633_05505"
     CDS             1537..2190
                     /locus_tag="VF633_05505"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8470549.1"
                     /translation="MTDSPEKTEPNTPLVPAWAEGLEKTPPWKDLGHVVVHETPWMKV
                     TEHQAIAPTGQDATYGVLRFKNIATGVLPIHPDGTVTLVGQARFARANYSWEMPEGGA
                     PFGEDPLDAVKRELAEEAGLEAANWQLALKVEVSNSITDEIGYTWLAWDLKPVPVAPD
                     PTEVMTVVRVPFLSLLAEIERGSILDAFTVATAYRAYHMAREGLLPGWLAHAMLTRV"
     gene            2217..3059
                     /gene="cysE"
                     /locus_tag="VF633_05510"
     CDS             2217..3059
                     /gene="cysE"
                     /locus_tag="VF633_05510"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX8470550.1"
                     /translation="MSTQPRLEVIEGQMPAPDSVWTQLRASAEEAARSEPHLGSLMNA
                     TILSHLDLANALSFQIARKLGDAELAQMSVREVCAFAFWSEPSIVAAAEADLKAVAER
                     DPAIRSLLQPFLYFKGFQALQASRVAHWLWTQGRDTLAFHFQSRISELFQIDIHPGAK
                     LGKGLFLDHGTGIVIGETAVVGDDVSMLHGVTLGGTGAERGDRHPKIGKGVLLGAGAK
                     VLGNIEIGDYAKVASGSVVLKPVPTGCTVAGVPARLVNCPTGAEPARTMDHTLADVVY
                     DFSI"
     gene            3133..3345
                     /locus_tag="VF633_05515"
     CDS             3133..3345
                     /locus_tag="VF633_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEX8470551.1"
                     /translation="MNTNDMKRIEAHLKKTFNSAGIIVKPRPKVTDSAEVYVGDEFIG
                     VVFDDEDEDGSFMFEMAILAEDLAAD"
     gene            complement(3449..4048)
                     /locus_tag="VF633_05520"
     CDS             complement(3449..4048)
                     /locus_tag="VF633_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470552.1"
                     /translation="MAKAPSKSAPAKPAAASKPKPAAKSSAATTSTAKAADVTAAPKP
                     AKAPAAKVAPKTATAKTAAPKAAAAPKPAATKSAAKPAVKTVTAPKAAVPKATTKVAT
                     PVKTTPAPAAKTARTVKPAAAPTAKAAAPKAEVPKAEAPAPKPAQAPKPAEAPKPVPA
                     TAAPKAEAAPAPKPTAKPAEPSKPKSFLDKIADAIFGKR"
     gene            4135..5004
                     /locus_tag="VF633_05525"
     CDS             4135..5004
                     /locus_tag="VF633_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX8470553.1"
                     /translation="MSQTAVQIIAHGARADAEAAAEALDADVMLEGATYSILEEDEDK
                     GIWRIDAFPTTDEESEGIQARLAEHPGVTVKVEVLADADWLAMSLSGLPPVKAGRFFV
                     YGAHDQGQVPLSAVALKIDAGAAFGTGHHGTTVGCLLAYDELLKRETFDRVLDVGCGT
                     GVLAIAAARTGSKVAVGTDIDAPSVRIANENAKLNQAKASFVHASGLNDQRVRKHGPY
                     DLVFANILAPPLVMLSQDIKMALRIGGVAILSGLLRTQERRVTAAYLSRGFRLERRIH
                     RDAWSALVLRRMA"
     gene            5070..5546
                     /locus_tag="VF633_05530"
     CDS             5070..5546
                     /locus_tag="VF633_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470554.1"
                     /translation="MSQYDPDLDRQRPLQGESVYTETVIVRRESNTGWWIAGGLAALI
                     LIAVLWILFARSTPTADDTEARLAEAQAAQAVAEAQASSNAAAVQGQIAGGQQSIELA
                     RVDAARAQAEAQRASSEARAAEARAVGPAIITAPATPSSPPPSGGVAVITPTSPQP"
     gene            5596..5994
                     /locus_tag="VF633_05535"
     CDS             5596..5994
                     /locus_tag="VF633_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEX8470555.1"
                     /translation="MPLHMIKLCVGVAEVETLEQRSLKNGGVVVQTKMTPKRADEMED
                     GGSLYWVMKGSVVGRQTILDIKTLGEGPAKHCLIQLAPEVIRTAPQARRPFQGWRYLE
                     AKDAPPDLTVMEAGDLPDDLAQQLREMGAW"
     gene            6184..>6496
                     /locus_tag="VF633_05540"
     CDS             6184..>6496
                     /locus_tag="VF633_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470556.1"
                     /translation="MLRLSELKLPLDHVPEALPAAIAARLGVPPQDLVSWSIWKRAHD
                     ARRKSAILKVYIVDVEVRDEAAVLARFKDDVHVQPTPDVAYRPVAVAPKTQRLRPVVI
                     GA"
BASE COUNT         1058 a         1986 c         2273 g         1179 t
ORIGIN      
        1 cacgctttcc tgatcaagcc ctgcgagctc gttgcgagcg ccctcggtgg ccaggatcaa
       61 ctcgtcgagc ttggcttgaa gggccgccgt gtcacggttt tggcctcgct ggatgatcag
      121 ggtcatagcg accgcgacta gggtcgcgac ggcatgaaga tcgaagctgt ggggttcgac
      181 cctttgccag acgacggcgc agatcaacaa aaccacgagg ctggcaggat ggccgagggc
      241 gccgacggtg gcggtcatgg cgcggtgcag ggggctggac atccccgtca gcgctcgcga
      301 acggcgtggg ttccgccgat atcatgcaag ccgctgaaat ggcgaagtct ccccgcgcgg
      361 cgggatgccg gctcgcgcgg ggagggtcga ctgtctttga gcggccggct tagtcttcgt
      421 cttcctcggc cgcttcagcc tcttccttgg tcttgaagct gacgccctcg tcctcggccg
      481 gcggggcgta gacggtgaag atcttcaggt cgtcttcgcc cttgttgatg atgttgtgct
      541 tggcgcccct tgggatgacc acgaggtggt tggcggtgac cttggtgcgc gatccatcaa
      601 ccacgacctg gccttcgccc gatacgacga aagtcgtctg gtcggcgtcg tgtgtctctt
      661 caccgatgtc ttcgccggcc gggatgctca tcatcacgat ctgcaccagc tcgtcgtagt
      721 aagcttcctt ttgccagttc ttgttggtct cggtcagctt gatgatgtct ttgttgaata
      781 cggccacggc gcgctcctgc ggttgggtga tggagtagcc ttcaacccgc tacgcttcgc
      841 cgggttgcac cgctccatct ccaatcaggg cagggtagtc gtcagggtcg acaggtcggg
      901 gcgaacgcgt tcgagcgggg gctcgacggt ggcgccgatg tggataaagc cggcgacctt
      961 ttcaccggcc ttcacgccca gcagcgtgag ggcgtccggg tcgtagctgt accagtcggt
     1021 gatccagcag gaggcatagc cgagagcgtt ggcggcgtgc tcgatgttca tgcagacggc
     1081 gccggaagac agctcctgct cccagactgg tttgggcccc gggcgggccg tcgagatgac
     1141 gaggatcgag acgggggcgg cggtcagctt ggctagcacc gcctgagcct tgacgccgag
     1201 ttccttgcgg acggcgagga ccgacaggct ttcgccgagc gtcgctcggg tttgagggcc
     1261 catgaccacg aagcgccaag ggaagagctt gccgtgatcg ggagtgcggg cgccgaggcg
     1321 gatgatctcg gcgacctcgg ctgcgtccgg accgggggcg gtgagggtct gggccggggc
     1381 ggaacggcga gtggccagcc ggttcagcag ttcgtctgac cggaccggct ggggcaggag
     1441 atcgccgagg tcggcgaccg gagcgaaggc tgggactgcg aactgggtca tgccgtctag
     1501 ctaggcgtcg gcatcggctt gcgccatagg acgggcgtga ccgattcccc cgaaaagacc
     1561 gaaccgaata ctccgcttgt gcctgcgtgg gccgaagggc ttgagaagac gccgccctgg
     1621 aaggatctgg ggcatgtcgt ggtgcacgag acgccgtgga tgaaggtcac cgagcatcag
     1681 gcgatcgctc cgaccgggca ggacgcgacc tatggcgtgc tgcggttcaa gaacatcgct
     1741 accggcgtgc tgccgatcca tcccgacggg acggtgacgc tggtcggtca ggcccggttc
     1801 gcgcgggcca actattcgtg ggagatgccc gaaggcggcg cgccgttcgg tgaggatccg
     1861 ctggacgcgg tcaagcgtga gctggccgag gaggcggggc tggaggctgc gaactggcag
     1921 ctggcgctca aggtcgaggt gtcgaactcc atcaccgacg agatcggcta cacgtggctg
     1981 gcgtgggacc tgaagcccgt gccggtcgcc cccgatccga cggaggtcat gaccgtggtg
     2041 cgggtgccgt tcctgtcttt gctggcggag atcgagcgcg gttcgatcct ggatgcgttt
     2101 acggtagcga cagcgtatcg ggcctaccat atggcgcgtg agggtctgct gcccggctgg
     2161 ctggcgcacg ccatgctgac gcgggtataa gaccagacgt ccggagctac gccgccatgt
     2221 ccacgcaacc ccgacttgag gtgatcgaag gccagatgcc tgcgccggat agcgtctgga
     2281 cgcagcttcg cgcctcggct gaggaagctg cacgcagcga gccgcatctc gggtcgctga
     2341 tgaacgcgac gatcctgtcg cacctcgacc tcgccaacgc tctcagcttc cagatcgcgc
     2401 gcaagctcgg cgatgcggaa ctggcccaga tgagcgtgcg tgaagtgtgc gccttcgcct
     2461 tctggtccga gccgtccatc gtcgcggcgg ccgaggccga ccttaaggcg gtcgccgagc
     2521 gcgatccggc gatacggtcg ctgttgcagc cgttcctcta tttcaagggg ttccaggcgc
     2581 tgcaggcgtc gcgggtggcg cactggctgt ggacgcaggg gcgcgatacg ctggcgttcc
     2641 attttcagag ccgcatttcc gaactgttcc agatcgacat tcatccgggc gccaagctgg
     2701 gcaagggcct gttcctcgac cacggcacgg gcatcgtcat cggcgagacg gcggtggtcg
     2761 gcgacgacgt gtctatgctg cacggcgtga cgctgggcgg gacaggggcc gagcgcggcg
     2821 atcgtcaccc caagatcggc aagggcgtgc tgctgggtgc gggcgccaag gtcttgggta
     2881 atatcgagat tggcgattac gccaaggtcg cctcaggctc ggtggtgctg aagccggtgc
     2941 ctacgggttg cacggtcgcg ggcgtgccgg cgcggctggt caactgtccc acgggcgccg
     3001 aacctgcgcg gacgatggac catacgctgg ccgacgtggt ctacgacttc tcgatctgag
     3061 ggtttccccg gcaggcgcca gcgactactg tccgccacca aatcacactg atctaaactt
     3121 ccgaggcccg cgatgaacac gaacgacatg aagcgcatcg aggcgcacct gaagaagacc
     3181 ttcaactccg caggcatcat cgtgaagcct cgtcccaagg tcacggactc ggctgaggtc
     3241 tatgtcggcg atgagttcat cggcgtggtg ttcgacgacg aggatgagga cggcagcttc
     3301 atgttcgaaa tggcgatcct ggccgaagac ctcgccgccg actgagcgga cggacattcc
     3361 agacaaggaa aacccccggg cggttcgcac cacccggggg ttttttattt tggcccatcg
     3421 cccttagcgc tacttgaggc ccgtgacctc agcgcttgcc gaagatggcg tcggcgatct
     3481 tgtcgaggaa gctcttgggc ttggacggct cagcaggctt tgctgtcggc ttcggagccg
     3541 gagcggcttc agccttcgga gcagcggtgg caggcacggg cttgggagcc tcagccggct
     3601 ttggcgcctg ggcgggctta ggagcaggcg cttcagcctt gggaacttca gccttcggcg
     3661 cagcggcctt cgcggtggga gccgccgcag gcttcacggt gcgagccgtc ttagccgccg
     3721 gggcaggcgt ggtcttcacc ggtgtggcga ccttggtcgt agccttggga acagcggcct
     3781 tgggagcagt taccgtcttt acggctggct tggctgcaga cttggtcgcc gccggcttgg
     3841 gagcggccgc ggctttcgga gcggcggttt tcgcggtagc ggtcttaggc gcaaccttcg
     3901 cagcgggagc cttggcaggc ttcggagcgg ccgttacgtc agcagctttc gcggtcgaag
     3961 tcgtcgcagc gctcgacttt gccgccggtt tcggcttgga agcggctgcc ggtttcgccg
     4021 gcgcagattt agacggtgct ttagccatga tatccccgac ccctcggttg gttggaacac
     4081 acggatgtgg gttcgcgact gtttcgattc gttatgatac cggcgtttga gcgaatgtcc
     4141 caaactgcag tccagataat cgcacacggc gcgcgcgcgg acgccgaagc cgccgccgag
     4201 gcgctcgacg ccgacgtcat gctggagggt gcgacctact ccattctcga ggaagacgag
     4261 gacaagggca tctggcgcat cgacgccttc ccgacgacag acgaggagtc cgaaggtatc
     4321 caggcccgcc tcgccgaaca tccaggcgtg accgtcaagg tcgaggtgct cgcggacgcc
     4381 gactggctgg ccatgtcact gtcaggcctg cctccggtaa aggccgggcg tttcttcgtc
     4441 tatggcgcgc atgatcaggg gcaggttccc ttaagcgccg tggccctgaa gatcgatgcg
     4501 ggagcggcct tcggtaccgg ccaccacggt acgaccgtcg gctgcctgct ggcctatgac
     4561 gaactgctga agcgcgagac gttcgacagg gttctggacg tcggttgcgg cacgggtgtg
     4621 ctggccattg cggcggcaag gaccggctcc aaggttgcgg tcggcaccga catcgacgcg
     4681 ccttcggtgc ggatcgcgaa cgagaacgcc aagctcaacc aggccaaggc gagcttcgtc
     4741 cacgcttccg gcctgaacga tcagcgggtg cgcaagcacg gaccctatga tctggtgttc
     4801 gccaacatcc tcgccccgcc gctggtgatg ctgtcgcagg acatcaagat ggcgctgcgt
     4861 atcggcgggg tggctatcct gtcaggcctg ctgcggacgc aggaacgccg ggtgaccgcg
     4921 gcctatttgt ccaggggttt ccggctggaa cgccgcattc accgtgacgc atggagcgcc
     4981 ctggtgctgc gccggatggc ctgaccgaag gtgtgagggg aacctgtgcc gttagcgacc
     5041 gttgcagcgg atcgatggtg gaggaaccca tgagccagta cgaccccgat ctcgaccgcc
     5101 aacgtccgct ccagggcgaa tccgtctaca ctgagaccgt aatcgtccgc cgcgagtcca
     5161 acaccggctg gtggatcgcc ggcgggctgg cggctctgat cctgatcgcc gtgttgtgga
     5221 ttctgttcgc gcggtccacg ccgacggccg acgataccga ggcgcggttg gcggaggcgc
     5281 aggcggctca ggccgtcgcc gaggctcagg cttcgtccaa tgcggctgcc gttcagggtc
     5341 agatcgccgg tggccagcag agcatcgagc tcgcgcgggt cgacgccgcc cgggcccagg
     5401 ccgaggctca gcgcgccagt agtgaggccc gtgcagccga ggcgcgcgct gtcggccctg
     5461 caatcatcac ggcgccggcc acgccttcat cgcccccgcc gtccggcggc gttgcggtca
     5521 tcacgccgac ctcgccacag ccctgaatgc agtgacgctt gcctccgagg gggcaggaga
     5581 tgctagaggc gcgtcatgcc tcttcatatg atcaaactgt gcgtcggcgt cgccgaggtg
     5641 gagacgctcg agcagcgctc gctgaagaat ggcggcgtcg tcgtccagac caagatgacg
     5701 ccgaagcgcg ctgacgaaat ggaagacggc ggatcgcttt actgggtgat gaaagggtct
     5761 gtggtcggtc gtcaaacgat cctcgacatc aagaccctcg gcgagggtcc agcgaagcat
     5821 tgcctgatcc aactggcccc cgaggtcatc cgcacggcac cgcaggcgcg gcgccctttt
     5881 cagggctggc gctatctgga agccaaggat gcgccgccgg atctgacagt gatggaggcg
     5941 ggggatctgc ccgacgatct ggcgcagcaa ctgcgtgaga tgggcgcctg gtaggaaacg
     6001 gtgaggctga tcgcctggcg aggagcctac catgaccgcc gtggacaagc tggcgcgcaa
     6061 gtcgctgagg tagctcagga gccaggctga ccagaccgat gtgcggtatg ggcgggaaga
     6121 cgacttcggc cgcccttatc ttgcctcatg cgtttgacgt cgcgagcggc tacaaccccg
     6181 ccgatgttgc gtctctcaga actcaagctg cccctcgacc acgtgccgga ggccttgccc
     6241 gccgcgatcg ccgcccgtct gggcgtgccg ccgcaggacc ttgtatcgtg gtcgatctgg
     6301 aagcgggcgc acgacgcgcg caggaaatcg gcgatcctga aagtctacat cgtcgacgtc
     6361 gaggtcaggg atgaggcggc ggtgctggcc cgcttcaagg acgatgtgca cgttcagccc
     6421 acgccggacg tcgcctatcg ccccgtcgcg gtcgcgccca agacccagcg cctgaggccg
     6481 gtcgtgatcg gggccg
//
LOCUS       DASVTM010000205         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_657346,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000205 DASVTM010000000
VERSION     DASVTM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657346"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VF633_05545"
     CDS             <1..1156
                     /locus_tag="VF633_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX8470557.1"
                     /translation="YFSATSAHTCTGDGNAMVLRAGLPLQDMEFVQFHPTGIYGSGCL
                     ITEGARGEGGYLTNSEGERFMERYAPSAKDLASRDVVSRSMTMEIREGRGVGPKKDHI
                     FLHLDHLDPKILHQRLPGISESAMIFAGVDVTKEPIPVIPTVHYNMGGIPTNYHGEVL
                     TKVGDNADTVVPGLMAVGEAACVSVHGANRLGSNSLTDLVVFGRAAGLRAGEVVNKAA
                     PVPLAPESATAPHLARLERLRNANGTSSTAHLRGEMQAAMQSDAAVFRTGKTLDEGVS
                     KLRAIHARGADIKTTDRGLIWNTDLVETLEYDNLIDQALVTIEAAANRLESRGAHARE
                     DFPDRHDDEWMKHTLTWKKPGEPVAIDYRPVHTYTMSSDIEYIKPKARVY"
     gene            1158..1955
                     /locus_tag="VF633_05550"
     CDS             1158..1955
                     /locus_tag="VF633_05550"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX8470558.1"
                     /translation="MAAFTLPIGSRPKKGKVYKAPAGAQNVKTYKVYRYDPNIDENPS
                     WDEYQVSADDHGPMLLDALIHIKNEIDPTLSFRRSCREGICGSCSMNIDGRNGLACTK
                     GWSECSSSTIAISPLPHQPVVKDLVTDLTLFYAQYDSIKPYLQSDDPDPTTERLQSPE
                     DRAKLDGLYECILCACCSTSCPSYWWNAGEYLGPAALLQSYRWIADSRDDATQSRLDD
                     LEDPFKLYRCHTIMNCAQVCPKGLNPAKAIAETKKLMIAPDRKKTAA"
BASE COUNT          396 a          723 c          609 g          336 t
ORIGIN      
        1 ctacttcagc gccacctcgg cccacacctg caccggtgac ggcaacgcca tggtgttgcg
       61 cgccggcttg ccgctgcagg acatggagtt cgtccagttc cacccgaccg gcatctacgg
      121 ctcgggctgt ttgatcaccg aaggcgcgcg cggcgaaggc ggttacctga ccaactccga
      181 aggcgagcgg ttcatggaac gctatgcccc gtcggccaag gaccttgcct cgcgcgacgt
      241 ggtctcgcgc tccatgacca tggaaatccg cgaaggccgc ggcgtgggtc cgaagaagga
      301 tcacatcttc ctgcacctcg accacctcga tccgaagatc ctgcaccagc gcctgccggg
      361 catctccgag agcgccatga tcttcgccgg cgtcgacgtg accaaggagc ccatcccggt
      421 cattccgacc gtccactaca atatgggcgg catccccacg aactatcacg gcgaggtcct
      481 gaccaaggtc ggcgacaacg ccgacaccgt ggtgcccggg ctgatggccg tcggtgaggc
      541 cgcctgcgtc tcggtccacg gcgccaaccg tctgggttcg aatagcctca ccgatctggt
      601 cgtgttcggt cgcgccgctg gtctgcgggc cggtgaagtg gtcaacaagg ccgccccggt
      661 gccactcgcc ccggaatcgg ctacggcccc gcaccttgcc cgcctcgagc gcctgcgcaa
      721 cgccaacggc acgtcctcga ccgcgcatct gcgtggcgag atgcaggccg ccatgcagtc
      781 ggacgccgcc gtgttccgca ccggcaagac gctggacgag ggtgtctcca agctccgcgc
      841 cattcacgcc cgcggcgccg atatcaagac caccgatcgc ggcctgatct ggaatacgga
      901 tctggtcgag actctggaat acgacaacct gatcgatcag gcgctggtca ctatcgaagc
      961 tgcggccaac cgtctggaaa gccgtggggc ccacgcccgc gaggacttcc ccgaccgtca
     1021 cgacgacgaa tggatgaagc acaccctgac ctggaagaag cccggcgagc cggtcgcgat
     1081 cgattaccgt cccgtccaca cctacacgat gtccagcgac atcgagtaca tcaagcccaa
     1141 ggcgagggtt tactgagatg gctgctttca ccctcccaat cggatcgcgc cccaagaagg
     1201 ggaaggtcta caaggctccc gcaggcgccc agaacgtcaa gacgtacaag gtctatcgtt
     1261 acgacccgaa catcgacgag aacccaagct gggacgaata tcaggtctcg gccgacgacc
     1321 acggcccgat gctgctggat gccctgatcc acatcaagaa cgagatcgac ccgaccctgt
     1381 cgttccgccg gtcttgccgc gagggcatct gcggttcctg ctcgatgaac atcgacggcc
     1441 gcaacggtct ggcctgcacc aagggttgga gcgagtgctc gtcgtcgacc atcgccatct
     1501 cgcccttgcc gcaccagccg gtggtcaagg atctggtgac ggacctgacg ctgttctacg
     1561 cccagtacga ctcgatcaag ccctacctcc agtccgacga tccggatccg accaccgaac
     1621 ggctgcaaag ccctgaggac cgggccaagc ttgacggcct gtacgaatgc atcctgtgcg
     1681 cctgctgctc gacgtcctgc ccgagctact ggtggaacgc cggcgaatac ctcggcccgg
     1741 cggccctgct gcagtcctat cgctggatcg ccgacagccg tgacgacgcg acccagagcc
     1801 gcctcgacga cctcgaggat ccgttcaagc tctatcgctg ccacacgatc atgaactgcg
     1861 cccaggtctg ccccaagggc ctgaaccccg ccaaggccat cgccgagacg aagaagctga
     1921 tgatcgcgcc cgatcgcaag aagacggcgg cctgatccac cacgcatggc cgggattctg
     1981 tcctcctatg ggacgttgca ggacccggtg gtgcagatca cccattttcg gacggacggt
     2041 ggtccgcaag gctgacgtgc gtct
//
LOCUS       DASVTM010000206         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_75303, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000206 DASVTM010000000
VERSION     DASVTM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75303"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="VF633_05555"
     CDS             complement(<1..2158)
                     /locus_tag="VF633_05555"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX8470559.1"
                     /translation="MSDAVIADPTTGEEVPSSRAPQLALSEELLASPTRFFNREISWL
                     KFNARVLEEAANPIHPLLERLRFLSISANNLDEFFMTRVAGLKGQVRERVRVRSSDGL
                     TPAEQLEKVNVGAGELMAEQQSRWGQLRKELTEAGIEVIHSAKITKTEKDRLEPEFLN
                     QLFAVLTPLAIDPAHPFPFLPNLGFSLVLKLRRRADNRVLYALVPVPTQVRRFWELPV
                     DGRSKPGRRRFVTLESLLLLFIDHLFPGCEVLEKGLIRLIRDSDIEIEEEAEDLVLEF
                     EEALKQRRLGEVVRIKIESSTPDDLREFIVSELKAAPQDVVLVHGMLGLAQLSELIPN
                     GHPELKFPGYEPRYPERVRDQGGDVFAAVREKDILIHHPFESFDVVVQFLRQAARDPN
                     VIAIKQTLYRTSKDSPIVAALIEAAENGKNVVALVEIKARFDEEANLRWARAMERAGV
                     HVVFGFVEYKTHAKLSIVVRREGEVLKTYCHFGTGNYHPVTAKVYTDLSLFSSDPVLA
                     RDAGRVFNYITGYAEPDELEALVVSPLNMKATLIELIGKEMSAAAMGKPAAIWVKLNA
                     LVDPEIIDALYRASQWGVRIELVVRGICCLRPGVPGLSENIRVKSIVGRFLEHSRIIC
                     FANGKDLPHDKAKLYISSADWMERNLNRRVELMTPIRNATVHDQVLDQIMVANLKDDA
                     QSWVLDAAGDYHRVAPLDPERPFSAHQYFMTNPSLSG"
BASE COUNT          392 a          690 c          761 g          421 t
ORIGIN      
        1 gacccgacag gctggggttg gtcatgaaat actggtgcgc ggagaacggc cgttcgggat
       61 cgagcggcgc gacgcggtga tagtcgccgg cggcgtccag cacccagctc tgggcgtcgt
      121 ccttcaggtt ggcgaccatg atctggtcca gcacctgatc gtggacggtc gcgttccgga
      181 tcggcgtcat cagttcgacg cggcgattga ggttgcgctc catccagtcg gccgacgaga
      241 tgtagagctt ggccttgtcg tgcggcaggt ccttgccgtt ggcgaaacag atgatgcggc
      301 tgtgctcgag gaagcggccg acgatcgact tgacccggat gttctccgac agacccggca
      361 cgcccgggcg caggcagcag atgccgcgca cgaccagttc gatgcgcacg ccccactggc
      421 tggcgcgata cagggcgtcg atgatctcgg gatcgacgag ggcgttgagc ttgacccata
      481 tggccgcagg cttgcccatc gccgccgccg acatctcctt gccaatcagc tcgatcaggg
      541 tcgccttcat gttgagcggc gagaccacga gagcctccag ctcatcgggc tcggcgtatc
      601 cggtgatgta gttgaacacc cgaccggcat cgcgagcgag cacggggtcg gagctgaaca
      661 ggctgaggtc ggtgtaaacc ttggccgtga ccgggtgata gttgccggtg ccaaagtggc
      721 agtaggtctt caggacctcg ccctcgcgcc gcacgacgat gctgagcttg gcgtgggtct
      781 tgtactcgac gaagccgaag acgacgtgga cgcccgctcg ctccatggcc cgggcccaac
      841 gcaagttggc ctcttcgtcg aaccgggcct tgatctcgac caaggcgacg acgttcttgc
      901 cgttctcggc cgcctcgatc agggccgcga cgatcgggct gtccttggag gtccggtaga
      961 gcgtctgctt gatggcgatg acgttgggat cgcgagccgc ctgacgcagg aactgcacca
     1021 ccacgtcgaa gctctcgaac gggtggtgga tcaggatgtc cttctcgcgc acggcggcga
     1081 agacgtcgcc gccctgatcg cggacgcgtt cgggatagcg cggctcatag ccggggaatt
     1141 tcagctcggg gtggccgtta gggatcagtt ccgacagttg ggccagaccc agcatgccgt
     1201 gcaccagcac gacatcctgc ggcgcggcct tgagttcgct gacgatgaat tcgcgcaggt
     1261 cgtcaggcgt cgaggactcg atcttgatgc gcacgacctc gccgagacgg cgctgcttca
     1321 gagcctcctc gaactcgagg accagatcct cggcctcttc ctcgatctcg atgtccgagt
     1381 cgcggatcag acggatcaaa cccttttcaa ggacttcgca gcccgggaac aggtggtcga
     1441 tgaacagcag cagcaggctt tccagcgtca cgaaccggcg tcgacccggc ttggagcggc
     1501 catcgaccgg cagctcccag aagcgccgca cctgcgtcgg caccggcacc aaagcgtaga
     1561 gcacccggtt gtcggcccgg cgacgtagct tcagcaccag cgagaagccg aggtttggca
     1621 ggaacgggaa ggggtgagcc ggatcgatgg ccaggggcgt caacaccgcg aacaactggt
     1681 ttaggaactc gggctcgagc cggtctttct cggtcttggt gatcttggcg gaatgaataa
     1741 cctcgatgcc ggcctcggtc agttccttgc ggagctggcc ccagcgggac tgctgttcgg
     1801 ccatcagctc gccagcgccg acgttgacct tttccagctg ttcggcgggg gtcaggccat
     1861 ccgaggaccg tacgcggacg cgttcgcgga cctgaccttt caggcccgcc acccgggtca
     1921 tgaagaactc atctagattg ttggccgaga tcgacaggaa gcgcaggcgt tccagcaagg
     1981 gatggatcgg attggcggct tcttccagca cccgggcgtt gaacttcagc cagctgattt
     2041 cgcggttgaa gaagcgcgtg ggcgacgcca gcagctcttc ggacagggca agctgcggcg
     2101 cgcgggaaga ggggacctct tctccggtgg tggggtcggc aatcacggcg tcggacatga
     2161 aatggttgtg gagcgcgatc gtgacggctg caacctcggt cttagctcgc ggaggtctct
     2221 tcttcgtttc cgggcgtgtc ttcctcatct tcgtgcgcgc ccag
//
LOCUS       DASVTM010000207         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_337360,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000207 DASVTM010000000
VERSION     DASVTM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337360"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /gene="tatC"
                     /locus_tag="VF633_05560"
     CDS             <1..517
                     /gene="tatC"
                     /locus_tag="VF633_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX8470560.1"
                     /translation="PIIAWQLYRFVAPGLYRNERGAFLPFLIAAPVLFVMGGALVYFV
                     MLPFVMLFTLSQQVVGDGIAVVQTTKISEYLSLVTSLLLAFGLCFQLPVVTTLLGLAG
                     ILTSKLMVASRRYAIVGVVVIAAVVTPPDPISQLMLAVPLVLLYEISIWCVRIIELRK
                     RKDDAVEGLAG"
     gene            683..2197
                     /locus_tag="VF633_05565"
     CDS             683..2197
                     /locus_tag="VF633_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX8470561.1"
                     /translation="MNAVSPRQPLDILSAVAAVSIVTLLVMIGVIALDDPEARGLVAP
                     VTVGLGAGALILFRQLRKNARALRMSEARALYAATHDSLTQLPNKALFLDRMACLPAD
                     VSIGVFCIGVDQFEELYDFLGHEVGDRTVAELSKRLTSACPETETVARIADGVFALFW
                     RDLTAAQAEGVAGQLMQLMAASGKAASKDAAVSVSIGVSFPGDIGDRRPEEGLRQARL
                     AMSVARKHGGGCRIFEPAMDNELRERKALEADLRTALANGGLALCYQPQINAKGVITG
                     VEALMRWNHPERGVVSPTLFVPLAENCGLSEAVDRFALKQAFIDSQRWPSIRTAINIS
                     ATQVRAGGLVAMLQGLLSETGASTRSLEIEITESVLLSDDAATYEILKAVRKMGFTLA
                     IDDFGTGYSSLGYLKRFPIDKIKIDRSFVMHLGMQPESDAIVRAIVEMAAALDLKVLA
                     EGVESRAQVDRLAIAGCGDIQGFYFSRPVEADAIERMLARDGARMAPRLAHASR"
     gene            complement(2182..3045)
                     /locus_tag="VF633_05570"
     CDS             complement(2182..3045)
                     /locus_tag="VF633_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX8470562.1"
                     /translation="MRLLNWLWNALPGPRPIPLKDLRRMDAAGLLRRSRAMASAVYVG
                     DRTVVCRVLSRYRLYVASDDVGFGVHVMLDGLWEGWLTAFMARRITPGMRIVDAGANH
                     GYYTLMFAHLTGSAGHVAAIEPNPRTASLLRRSIYANGFDPRVTLFEQAGGARDDVIL
                     HLQVPDHEPKNAHLVGEEAPGTVAVKGARLDTLLSDWPRVDFMKIDVEGFEESFLEGA
                     WSIVQRDLPTMVLEFNAVRCTDPAGLLDRLEALYGRIQVIGRDSRAVMADRARLLDPS
                     RVDDWMLYLEA"
     gene            complement(3144..4553)
                     /gene="glnA"
                     /locus_tag="VF633_05575"
     CDS             complement(3144..4553)
                     /gene="glnA"
                     /locus_tag="VF633_05575"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX8470563.1"
                     /translation="MSTADKILKEIKDKDVKYVDVRFTDTKGKLQHVTFDIDLVDEDF
                     LNEGTMFDGSSIAGWKAINESDMLLKPDLTTAYIDPFYQATTMFLFCDVMNPDTGEPY
                     NRDSRSMAKKALQYIQAAGVGDVVYFGPEAEFFIFDDVRWTTQPGHTSYSYDSIELPA
                     NTGSEYTEGNMGHRPGPKGGYFPVNPVDSGQDLRAEMLGVMRDMGMQPEKHHHEVAPA
                     QHELGLKFSDMLTMADRLQLYKYIIHNVAAAYGKSATFMAKPMFGDNGSGMHVHMSIW
                     KDGKPQFAGDKYAGLSQECLWYIGGIIKHAKAINAFSNSTTNSYKRLVPGYEAPVKLA
                     YSARNRSASIRIPWVSSPKGKRLEARFPDPMGNPYLTFTALLMAGLDGIENQIDPGAP
                     ADKNLYDLPPEERGSIPEVCGSLKEALENLDKDRAFLKKGGVMDDDFIDSYIELKMEE
                     VMRLQLHPHPVEFDMYYSC"
     gene            complement(4627..>4769)
                     /locus_tag="VF633_05580"
     CDS             complement(4627..>4769)
                     /locus_tag="VF633_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX8470564.1"
                     /translation="DDLYPAVVEAIQTAARTGKIGDGKIFVTEVTDVIRIRTGETGAQ
                     AV"
BASE COUNT          818 a         1452 c         1564 g          935 t
ORIGIN      
        1 cccgatcatc gcctggcagc tgtaccggtt cgtagccccg gggctgtatc gcaacgagcg
       61 cggggccttc ctgccgtttc tgatcgctgc gccggtgctg ttcgtgatgg ggggcgcgct
      121 ggtctatttc gtcatgctgc cgttcgtgat gctgttcacc ctgagtcagc aggtcgtcgg
      181 cgacggcatc gcagtcgttc agacgaccaa gatctcggaa tacctaagcc tggtgacctc
      241 actgctgctg gcgttcgggc tgtgcttcca gttgcccgtc gtgaccacct tgctcggatt
      301 ggccggaatc ctcacctcga agctgatggt cgccagccgc cgctacgcca tcgtcggcgt
      361 ggtcgtgatc gcggccgtgg tgacaccgcc ggacccgatc agtcagctga tgctcgccgt
      421 gccgctggtg ctgctctatg agatctcgat ctggtgcgtc cgcatcatcg agctgcgcaa
      481 gcgcaaggac gatgcggtgg agggattggc tggctgaacg gcttccgcag tcaggccggg
      541 ctcccgctgc cctggacaag cgggcgggag ggcaggcgtc tgagcgccgg gatattcggg
      601 cggcgataat actacctccg gtgtttacac gatgcgaagg attgaggggg aggataaagg
      661 tctccttggt ccggaagcct ccgtgaacgc agtttcccct cgccagccgc tggatattct
      721 cagcgcggtc gccgccgtct cgatcgtgac cctgctggtc atgatcgggg tgatcgcgtt
      781 ggacgacccg gaagctcgtg ggcttgtagc cccggttacg gtgggtctcg gagccggcgc
      841 gctcattctg tttcggcagt tgcgcaagaa cgcacgcgcc ctgcgcatga gcgaagcccg
      901 cgcgctgtat gcggcgaccc acgattccct gacacagttg ccgaacaagg ccctgttcct
      961 ggaccggatg gcctgtcttc cggccgatgt ttctatcgga gtattctgta tcggcgtcga
     1021 tcagttcgag gagctctacg atttcctcgg tcacgaggtg ggcgacagga ccgtcgccga
     1081 actttcgaaa cggctgacct cggcctgtcc ggaaaccgaa acggtcgcca gaatcgccga
     1141 tggtgtattt gccctgttct ggcgcgatct gaccgccgcc caggcagagg gcgtcgccgg
     1201 ccaacttatg caactgatgg ccgcatccgg aaaagctgca tcgaaggacg ccgctgtctc
     1261 ggtttccata ggcgtgtcgt ttcctggcga catcggcgac aggcgccctg aagaaggttt
     1321 gcggcaggcc cggctggcca tgtccgtcgc tcgcaagcat ggcggcggct gtcggatatt
     1381 cgagccggcg atggacaacg aactccgcga gcgcaaggcg ctggaggctg accttcgaac
     1441 cgccctggcg aatggtggcc tggccctctg ttaccagccc cagatcaacg ccaagggcgt
     1501 catcaccggg gtcgaggcct tgatgcgctg gaaccatccg gagcgtggcg tggtctctcc
     1561 cacgctcttc gttcccttgg ctgaaaactg cggcctgagt gaagccgtcg accggtttgc
     1621 attgaagcag gccttcatcg acagccagcg ctggccttca atccgcactg ccatcaacat
     1681 ctccgccacc caggtgcggg ccggcggcct ggtggcgatg ttgcagggac tgctgagcga
     1741 aaccggcgcc tccacccggt cgctggagat cgagatcacg gaaagcgtcc tgctgtcgga
     1801 tgacgcagcg acgtacgaga tcctcaaggc cgtgcgcaaa atgggcttca cgcttgccat
     1861 cgacgacttc ggcaccggct actccagcct tggctatctg aagcggtttc caatcgacaa
     1921 gatcaagatc gaccggtcgt tcgtgatgca tctggggatg cagcccgaaa gcgacgccat
     1981 cgttcgcgcc atcgtcgaga tggccgccgc gctcgacctg aaggtcctgg ccgaaggggt
     2041 ggaatcccga gcccaggtcg acaggctggc gattgccggc tgtggagata tccaggggtt
     2101 ctatttcagc cgtcctgtcg aagccgacgc gatcgagcgc atgctggccc gggacggcgc
     2161 ccggatggcg ccaagattgg ctcacgcctc gaggtaaagc atccagtcat cgacccggct
     2221 ggggtcgagc agtcgcgccc tgtcggccat gaccgcgcgg ctgtcgcgac cgatcacctg
     2281 gatgcggcca tagagcgctt ccaaccgatc cagcaggcct gcgggatccg tgcagcgaac
     2341 ggcgttgaac tcaagcacca tagtcggaag gtcgcgctgg acgatcgacc acgccccctc
     2401 gaggaaggac tcctcgaacc cttccacatc gatcttcatg aaatcgaccc gtggccagtc
     2461 ggacagcagg gtatccagcc gggcgccctt gaccgcgact gtcccgggag cctcttcgcc
     2521 gacgagatgg gcgttcttgg gctcgtgatc cggaacctgc agatgcagga tgacgtcgtc
     2581 cctggcgccc ccagcctgtt cgaacagggt cacgcgagga tcgaagccat tggcgtagat
     2641 tgatcggcgc agcaggctcg ctgttcgagg gttgggctcg atcgccgcca cgtgtcccgc
     2701 tgatccggtc aaatgggcga acatcagggt gtagtagccg tggttggcgc ccgcatcgac
     2761 gatccgcatc cccggcgtga tccggcgcgc catgaaggct gtcagccagc cctcccacag
     2821 accgtccagc atgacgtgga caccgaagcc gacgtcgtcg gaggcgacat agagccgata
     2881 gcggctgagc acccggcaga cgactgtccg gtcacccaca tagaccgcgc tcgccatggc
     2941 ccggctgcgt cgcagaaggc cagcggcgtc catgcgacgg aggtccttga gcgggatggg
     3001 tcggggaccg ggcagggcgt tccacagcca gttcagcagt ctcacacgcg tctccaaaaa
     3061 cgaaccggcc ggaacgacgg atgtcgtccc ggccggatgc cgtcaagatg tcaggcggga
     3121 ctgacgcccc gccgtcgaag ccatcaacag ctgtagtaca tgtcgaattc gaccgggtgc
     3181 ggatgcagtt gcagacgcat cacttcttcc atcttcagct cgatgtagct gtcgatgaag
     3241 tcgtcatcca tgacgccgcc cttcttgagg aaggcgcggt ccttgtcgag gttctcgagc
     3301 gcttccttga gcgagccgca gacctcgggg atcgagccgc gctcttcggg cggcaggtcg
     3361 tagagattct tgtcggcggg tgcgcccgga tcgatctggt tctcgatgcc gtccagaccg
     3421 gccatgagca gggcggtgaa ggtcagatag gggttaccca tcggatcggg gaagcgagct
     3481 tccagacgct tgcccttggg cgacgaaacc catggaatac ggatcgaggc cgagcggttg
     3541 cgggccgaat aggccagctt gaccggggct tcgtagcccg ggaccagacg cttgtaggag
     3601 ttggtcgtcg agttcgagaa ggcgttgatg gccttggcgt gcttgatgat gccgccgatg
     3661 taccagaggc actcctgcga cagaccggcg tatttgtcgc cggcgaactg gggcttgccg
     3721 tctttccaga tcgacatgtg gacgtgcatg cccgagccgt tgtcgccgaa catgggcttg
     3781 gccatgaagg tcgccgactt gccgtaggcg gcggccacgt tgtggatgat gtatttgtag
     3841 agctgcaggc ggtcggccat ggtcagcatg tccgagaact tcaggccgag ctcgtgctgg
     3901 gccggagcca cctcgtggtg gtgcttctcg ggctgcatgc ccatgtcgcg catcacgccc
     3961 agcatttcgg cgcgcaggtc ctgaccggag tcgaccgggt tgacggggaa atagccgccc
     4021 ttgggcccag ggcggtgacc catgttgcct tcggtgtatt ccgagcccgt gttggccggc
     4081 agttcgatcg agtcgtacga atagctggtg tggccaggct gggtcgtcca gcgcacgtcg
     4141 tcgaaaatga agaactcggc ttccgggccg aaatagacca cgtcgcctac accggcagcc
     4201 tgaatgtact gcaaggcctt cttggccatg gagcggctgt cgcggttata gggttcgccg
     4261 gtgtccgggt tcatgacgtc gcagaacagg aacatcgtcg tcgcctgata gaacgggtcg
     4321 atgtaggccg tcgtcaggtc aggcttcagc agcatgtccg actcgttgat ggccttccag
     4381 cccgcgatcg acgagccgtc gaacatcgtg ccttcgttca ggaagtcctc gtcgacgagg
     4441 tcgatgtcga aggtgacgtg ctgaagcttg cccttggtgt cggtgaaccg gacgtcgacg
     4501 tacttaacgt ccttgtcctt gatctccttg aggatcttgt cggccgtgct cattctcgat
     4561 gggtcctgtt tgttctgggg agagagtggc caccccccga cagggacgac cggtaggtgt
     4621 tgaacgctag acggcctggg ccccggtctc gcccgtacga atacggatga catcggtcac
     4681 ctcggtgacg aagatcttgc cgtcgccgat cttgccggtg cgagccgcgg tctggatggc
     4741 ctcgacgact gcagggtaga ggtcgtctg
//
LOCUS       DASVTM010000208        10980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_287236,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000208 DASVTM010000000
VERSION     DASVTM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287236"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1582)
                     /locus_tag="VF633_05585"
     CDS             complement(131..1582)
                     /locus_tag="VF633_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX8470565.1"
                     /translation="MFWRGVWGYLPANIVQGVVGFLAIFIFTRLLTPEDFGRYALAFS
                     IVTLCHVAVFSWLEAAMARFWAAQQAGADMAAHFASIYRTAFILIALFVPVAAVVLWF
                     APIEPLFKAAIGVGLLGSPARCLAKLAQERFRAAGEVSRSAMLDMATTIGGLCIGIAF
                     AFCGAGGAAPLVGMGLAPLAALPFILPGELRQAKTGVVDTARLKSYAGYGYPIAASLT
                     LALVLASTDRFLLAAFMDEAAVGAYHAGYSIANRTLDVLFLWLGAAGQPALVMALERG
                     GLQKLKEAAREQASTFFLIGFPAAAGVALVARPLGEVMIGESLRVAAAQVAPWVALSA
                     LLYGLTAYYFGQAFTLGRKTKLLLVAMTVPALANVLLNLFLIPRFGVVGAAWSTAISF
                     GLGMVTTMLLGRKVIPLPVPWEPLVRCGVATGIMALVVWSLPPVGGLAELAMDASVGG
                     IVYAAMALTINAAGVRDIAQGLLQKFRARRVAA"
     gene            complement(1590..3233)
                     /locus_tag="VF633_05590"
     CDS             complement(1590..3233)
                     /locus_tag="VF633_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX8470566.1"
                     /translation="MPNTLNVTRLPTASPPVEADDAARAGIMPVEPDVRAIQALAARA
                     MVPPPASAGTGLASIGDSANEGASRRGPFRPEIWLNARQKNASRLSVHYFRTFDVLAV
                     AAVTAACAWIMAPAGLMALELSRAVPMVLGCVVALGLVKSLGLYDFARDQSPTRHVVA
                     LNIIVLVSGVLALGAGWILQGPVIEIRALAVWMAATLATLIALHLGWSLLVSDWRAKG
                     ALTPNIVVVGATQHAERLIRDALKRRDINVLGVFDDRLARSPDSIEGVPVLGTAEALL
                     THKITPYVDRIVIAIDPEARERVRELTARLQALPNEVTLLVDPQGEEECTAALTKLAR
                     APLASLNSADADRRAFHKRLQDVIIGGLGLIAVSPVIGLCALAVKLDSPGPVFFRQRR
                     HGFNQEAIIVWKFRSMRQESTDATASRQVTADDDRITKVGKFLRKTSLDELPQLLNVL
                     KGEMSLVGPRPHAIGMKTGTVESSLLVAEYAHRHRIKPGMTGWAAIKGSRGPLDDAQA
                     VRRRVQLDVDYIERQSFWLDLWIIAITLPVLLGDRSAVR"
     gene            3401..3492
                     /locus_tag="VF633_05595"
     tRNA            3401..3492
                     /locus_tag="VF633_05595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3435..3437,aa:Ser,seq:gct)
     gene            complement(3549..4340)
                     /locus_tag="VF633_05600"
     CDS             complement(3549..4340)
                     /locus_tag="VF633_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470567.1"
                     /translation="MLSIMALLAFGSLAQTAETLRSSEVRAAEPVSLARRLLPPDTAD
                     RIIGGRVQRAWQPGETYSIGLWERPAVHTSGLCKRTVHFGRAMAPGVRTPPQQDAILQ
                     VQAFTTAVQYAPTYPREADDEVCQTAEGWIGSDEATVEPTLLMLTRLTEAMTQAAGRG
                     RLAFQISCMSEVLRACTNERRALAGLPLDKLVFILLRNTEYRNDPVQNGVQVRYMQPV
                     ADNRWPEAEVNFDYSEPDGRSWIIVLKGIDRLEAVETRRTTIIRH"
     gene            4649..4870
                     /locus_tag="VF633_05605"
     CDS             4649..4870
                     /locus_tag="VF633_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470568.1"
                     /translation="MNAPNDQLKLARHVLGWSEGEMARALRLAGTPEKAMARVREMEA
                     GKRDISGPVQVAVEAFMSGWRPSGWKPDA"
     gene            4993..5298
                     /locus_tag="VF633_05610"
     CDS             4993..5298
                     /locus_tag="VF633_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8470569.1"
                     /translation="MADLAQVTEHIRTAVGDNSGLGKTVKLDLGDAGKIYIDGASTPN
                     TVSNDDKPADATVSMNWDDFMSLSDGKLDPMMAFMQGKLKITGDMMIAQKLAPLLKR"
     gene            5441..6652
                     /locus_tag="VF633_05615"
     CDS             5441..6652
                     /locus_tag="VF633_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8470570.1"
                     /translation="MDFKHSELGLHWQEKLQRFMDDKVHPRTAEYWAQVHADHTVQPP
                     IMETMKAEAREAGLWNMFLPGDHGAGLTNLEYAPLAEIMGRALWSAEVFNCNAPDTGN
                     MEVLHMYGNEAQQAQWLKPLMAGTIRSAFLMTEPAVASSDATNIQTSIVREGDEYVIN
                     GRKWWSTNMGHPNVKIAIVMGKTDTSAATHSQQSQVLVPTDTPGFRIERMLTVFGYDE
                     RPIGHAEVVLDNVRVPVTNLIAGEGRGFEIAQGRLGPGRIHHCMRTIGAAELALELMC
                     ERLLNRTAFKKQLAEHSVWEQRVGEARTNIEMCRLMVLKAAWMIDQAGAKNAKSEIAQ
                     IKVAAPKMALQIIDDAIQAFGGAGVSGDTPLAELYAGVRTLRLADGPDEVHNRTIARL
                     EYAKHGGRPVR"
     gene            6853..9024
                     /gene="egtB"
                     /locus_tag="VF633_05620"
     CDS             6853..9024
                     /gene="egtB"
                     /locus_tag="VF633_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX8470571.1"
                     /translation="MNDLTQIQSSQSDDVALYRNVRDRMIELAKGLSAEDLAAQPMAD
                     ASPGKWHLAHTSWFFEAMILASEPNYQPVDPRFQQLFNSYYEALGRRVDRHERGLMTR
                     PGLDDVLAYRREIDRRMVDRLSRGLTSGAERYLFVLGLHHDQQHQELFLMDLLNLMSR
                     SPLDPAAYDVEPRDAPVQPSVGGVTRFDGGLITLGHDGEGFAFDNEGPAHQTFLRPFA
                     LANDLVTNADWIRFIDDGGYSRPEFWLADGWTTVKAEHWTAPLYWHAEEGGWSVFGLA
                     GAAPVDPSAPVRHVSFYEADAYARWAGKRLPTEAEWEYAAVTDAAPFSNLFGEVWQHT
                     GSAYTAYPGFRPTDGTASEYNGKFMANQMVLRGSCFATPLGHTRATYRNFFYPHQRWA
                     FMGLRLAEDIAADQSTVDEAGHDKGGETAAFRAEFIAGLSQPQKAVSPKWFYDAEGSR
                     LFEDITRLPEYYPTRQEASLLKALAPRLTEDFGNDAVLVEFGSGASEKTRILLDAAPT
                     LSTYIPIDISPDALNEAAERITAAYPSIEVVPVVGDFLNLPSLPELGRGRRIGFFPGS
                     TIGNLDRDAAVEFLKVARARLDTGSLFILGVDLVKAPELLIAAYDDAQGVTAAFNKNL
                     LVRANAELGTDFDLDAFEHLALWNEPESRMEMHLRSARDQRVTIGDTVIRFVKGETIH
                     TESSRKYTRASVITLAEAAGWTVSLFERSAEPSVALVLLSA"
     gene            complement(9112..9339)
                     /locus_tag="VF633_05625"
     CDS             complement(9112..9339)
                     /locus_tag="VF633_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX8470572.1"
                     /translation="MAEGQPNRPSGVSKRGFASMDPERQREIARKGGASVPSEKRSFS
                     QDRGLAAQAGRKGGEASHGSRRPEDVEGQAE"
     gene            complement(9436..9594)
                     /locus_tag="VF633_05630"
     CDS             complement(9436..9594)
                     /locus_tag="VF633_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470573.1"
                     /translation="MTYIDPGQPAEFPEPQNDQPDIDPAGVPDEMPPAQPGGGDEGDS
                     RTFDAKAI"
     gene            9732..10028
                     /locus_tag="VF633_05635"
     CDS             9732..10028
                     /locus_tag="VF633_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HEX8470574.1"
                     /translation="MTDTYSTNPDDVLSEDLQDETPSARLDRQADALLNDADDRAFAR
                     SEGIRKAVRSDLEHGKQWAADRTEMTRDAIRDEPMKTALYAVGIGVLIGLLLRR"
     gene            complement(10033..10743)
                     /locus_tag="VF633_05640"
     CDS             complement(10033..10743)
                     /locus_tag="VF633_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8470575.1"
                     /translation="MSQSPVPPPPIGVFITAVTPLQQNCTTVWCTRTMKAAVIDPGGS
                     VDAILSEVARRGLTLDQIWITHGHLDHAGGAAEMQEKSGVTITGPQLEDQFWIDGIPA
                     QGQMYGIPDARSFKPDRWLEDGDTVTLGETTWEVLHCPGHTPGHVIFFNREARFAQVG
                     DVLFQGSVGRTDFPRSDPDALIRSITEKLWPLGDGVTFVPGHGPVSTFGQERQTNPFV
                     SDRAMKGPRLEREASGPS"
BASE COUNT         2034 a         3752 c         3322 g         1872 t
ORIGIN      
        1 acgtctgcgg cttcgcgatc cagcgactgc agcaggtcgc gcgggtcatc ccgcaggaac
       61 ggcatcaaca ccgaaagctt cggatctgca cccgtccacg gcctattgtc gctgatccgg
      121 ggcgaagggc tcacgccgcc acccgccggg cgcgaaactt ctgcagcaga ccctgagcga
      181 tatcccgcac gcccgccgcg ttgatggtca gggccatggc cgcatagacg atcccgccaa
      241 ccgaggcgtc catcgccagc tcagccagcc cgccgacggg cggcaaggac cacaccacca
      301 gcgccatgat ccccgtcgcg acgccgcagc gaaccagcgg ctcccacggc accggcagtg
      361 ggatgacctt ccggcccagc aacatggtcg tgaccatccc cagcccgaag ctgatcgcag
      421 tcgaccaggc tgcaccgacc acgccgaaac gcgggatgag gaacaggttc agcagcacat
      481 tagccagcgc cgggacggtc atcgccacca gcaacagctt cgtcttcctg cccagcgtga
      541 acgcctgccc gaaatagtag gcagtcagcc cgtacaacag cgctgatagc gccacccacg
      601 gcgccacctg cgccgccgcg accctcaagc tttcgccgat catcacctcg ccgagcggtc
      661 tggccaccag agcaacgccc gccgcagccg ggaagccgat caggaaaaag gtcgaggcct
      721 gttcacgcgc ggcttccttc aacttctgca gcccgccacg ttccagcgcc atcaccagcg
      781 ccggctgacc cgcagcaccc agccacagga acagcacgtc cagagtccgg ttggcgatgg
      841 aataacccgc gtgataggcg ccgacggccg cttcatccat gaaggcggcc agcaggaacc
      901 ggtctgtcga ggccagcacc agcgccagcg tcagcgacgc cgcgatcgga tagccataac
      961 ccgcatagga tttgagccgt gcggtatcga ccacgcccgt cttcgcctgt ctcagctcgc
     1021 cgggcaggat gaagggcagg gcggcgagag gggccaaccc catcccgacc agcggcgccg
     1081 cgccgcccgc gccgcaaaat gcgaacgcta tgccaatgca cagcccgccg atggtcgtcg
     1141 ccatatcgag catggctgag cgagagacct cgcccgccgc ccggaaccgc tcctgcgcca
     1201 gcttggccag acaacgcgcc gggctgccca gcaggccgac gccgatggcc gccttgaaca
     1261 gcggctcgat cggcgcgaac cacagaacca ctgccgcaac cggcacgaac agggcgatca
     1321 ggatgaaggc cgtgcgatag atgctggcga aatgggcggc catgtcggcg cccgcctgct
     1381 gcgctgccca gaaccgcgcc atggcggcct cgagccagct gaacacggcg acatggcaaa
     1441 gcgtgacgat cgagaacgcc agcgcatacc gaccgaaatc ctcaggcgtc agcaggcgcg
     1501 tgaagatgaa gatggccaga aatccgacca ccccctgcac gatattggcg ggcagatagc
     1561 cccagacacc gcgccagaac atcagcgctt caccgcaccg ccgaccggtc gcccagcagc
     1621 accggcaggg tgatggcgat gatccacaga tccagccaga acgactgacg ctcaatgtaa
     1681 tcgacatcca gctgcacccg gcgacgcacg gcctgagcat cgtccagcgg tccgcgcgaa
     1741 cccttgatcg cggcccagcc ggtcatgccc ggcttgatgc ggtggcgatg ggcgtattcg
     1801 gccaccagca gcgaggactc gaccgtgccg gttttcatgc cgatggcgtg cgggcgcggt
     1861 cccaccagcg acatctcgcc cttcagcacg ttcagaagct gcggcagttc atcaaggctg
     1921 gtcttgcgca ggaatttgcc gaccttggtg atgcggtcat cgtcggcggt gacctgacgt
     1981 gaggcggtag cgtcggtgct ctcctgacgc atcgagcgga atttccacac tatgatggct
     2041 tcctgattga agccgtggcg gcgctggcgg aagaacaccg gcccgggact gtccagcttc
     2101 accgccagag cgcacagccc gatcaccggc gaaaccgcga tcagtccaag accgccgatg
     2161 attacgtcct gcagccgctt gtgaaaggcg cgtctgtcag cgtcggcgct gttcagcgat
     2221 gccagtggtg cgcgcgccag cttggtcagg gccgcggtgc attcttcctc gccctgcgga
     2281 tcgacgagca gcgtcacttc gttcggcagg gcctgcagtc tcgcagtcag ttcgcgcacc
     2341 cgctcccgcg cctcggggtc gatggcgatg acgatccggt cgacataggg tgtgatcttg
     2401 tgcgtcagca gcgcctcggc cgtccccaga accggcacgc cttcgatact gtcgggcgag
     2461 cgcgccaacc ggtcgtcgaa cacccccaga acattgatat cccgtcgttt caacgcgtcc
     2521 cggatcaacc gttcggcgtg ttgagtcgcc ccgaccacga cgatgttcgg cgtcaacgcc
     2581 cctttggccc gccaatcgga caccaacaga ctccagccga gatgcagggc gatgagcgtc
     2641 gccagggtcg cagccatcca gacggccagc gcccttatct cgatcaccgg accctgcagg
     2701 atccagcccg cgccaagcgc cagcaccccc gacaccagga cgataatgtt cagagcaacc
     2761 acatgccggg tcggagactg gtcacgcgcg aaatcataga ggcccagcga cttcaccaga
     2821 ccgagcgcga cgacacagcc cagaaccatc ggcaccgccc gcgacagctc cagcgccatc
     2881 aatccggccg gagccatgat ccaggcgcag gccgcggtta ccgccgcgac ggccagcaca
     2941 tcgaacgtcc ggaaatagtg cactgacagg cgcgaggcgt tcttctgacg cgcgttcagc
     3001 cagatttcag gccgaaacgg gccccgacgc gatgcgcctt cgttggccga atcgccaata
     3061 ctcgccaagc ctgtgcccgc agacgcgggt ggcggaacca tcgctctggc ggccagggcc
     3121 tggattgcgc ggacgtccgg ctcgacgggc atgatcccgg cccgcgccgc atcgtcggcc
     3181 tcgaccgggg gagaggcagt cggcagtcgg gtcacgttca gggtattggg catcagggtc
     3241 cgcgggttgg gaaactcggc gacatcctgc acagaccccg tggatgtcgc gttaacgccc
     3301 cagctttccc cattggatgg gggttatgga aaacttctcg ccccaaccgc gtcttgccga
     3361 ttgcgccgcg tcgcgacctt cgctatcgac gcctcctgcc ggagacgtgg ccgagtggct
     3421 gaaggcaacg gtttgctaaa tcgtcatacc ctgtaagggg tatcgagggt tcgaatccct
     3481 ccgtctccgc caggcccttc cctgaatcaa gagtaatgcc cgttcgggcc ccgcgtcggt
     3541 gaagatggtc agtgtcggat gatcgtggtc cgacgcgtct cgacggcttc cagcctgtcg
     3601 atccctttca aaacgatgat ccaggatcgg ccgtccggtt cggaatagtc gaagttcact
     3661 tccgcctcag gccagcggtt atccgcgacg ggctgcatat atcggacctg aaccccgttc
     3721 tgcaccggat cattccggta ctccgtgttt cgaagcagga taaacaccag cttgtctaac
     3781 ggaaggcccg ccagggccct gcgctcgttt gtgcaagctc gcagaacttc tgacatgcaa
     3841 ctgatttgaa acgccaaccg acctcggccc gctgcctgtg tcatcgcctc ggtcagtcgg
     3901 gtcaacatta gcaaggtggg ttcgacagtc gcctcgtccg agccgatcca cccctctgcc
     3961 gtctgacaaa cttcgtcgtc agcttcccga ggataggttg gagcatactg gacggcggtg
     4021 gtgaacgcct gcacctgtag gatggcgtcc tgctgtggag gagtcctgac tccgggagcc
     4081 attgcccgac cgaaatggac ggttcgcttg cacaatcccg atgtatggac ggccgggcgc
     4141 tcccacagtc cgatactata ggtttcaccg ggctgccatg cgcgctggac gcgcccgcca
     4201 atgatccggt cggctgtatc cggcggcagc agccgtcggg ccaatgatac cggctccgca
     4261 gcacgcacct cgcttgaccg caatgtttcc gccgtttggg caagactgcc aaaagcgagc
     4321 aaagccatga tgctgagcat ttggtcctcc ggagccggtc tgacaaactg cgcttcagcg
     4381 aaggggctgg cgaccacagg ataatcgttc ggcgcgttct ggttgtaaac acggtgtgat
     4441 gatcggaaga aatcgtagcg ctatttctgc acgtatagga atttgacccg catctcctga
     4501 agccggtttt ctccatcgtg gcgcgggcgc gttcgacacg gtcctttggg cgacgcctcg
     4561 ctgcaggcgg ggcccggaaa ccgttcgtgc gttgcctttc acggcttcgc gacccaaaag
     4621 gcgcgccctt tatgctagag cggatgtcat gaacgccccg aacgaccagt tgaagcttgc
     4681 ccggcacgtc ctgggctgga gcgaaggcga aatggcccgc gcgcttcgtc tggccggcac
     4741 gccggagaag gcgatggccc gtgtccgcga gatggaggcg ggcaaacgcg acatcagcgg
     4801 cccggtacag gtcgccgtcg aggccttcat gagcggctgg cgtccaagcg gctggaagcc
     4861 cgacgcctga tcctcatgct cgatgaacag gccgctgtcg atagataggc agaacttgac
     4921 gcgacgtgat cttggcagcc tccgcgcggg ggtttagggc tctctcaacc attgctttca
     4981 gggagacgac ccatggccga tctggctcaa gtcaccgaac acatccgcac cgccgtcggc
     5041 gacaactcgg gcctcggcaa gaccgtgaag ctcgacctcg gcgacgccgg caagatctac
     5101 atcgacggcg cttcgacccc gaacaccgtc tccaacgacg acaagcccgc cgacgcgacc
     5161 gtgtcgatga actgggatga cttcatgtct ctgtcggacg gcaagctcga cccgatgatg
     5221 gccttcatgc agggcaagct gaagatcacc ggcgacatga tgatcgccca gaagctggct
     5281 ccgctgctga agcgctgatg tccggccaat agaccagagt tccagacggc gcggctcctt
     5341 cggggccgcg ccgttttcgt ttcgacgcca atttcgcttc gatcaagctt ccggcgcgcg
     5401 gtttcgcgaa cgaccggggt cataaaaagg ggatacgcac atggacttca aacacagcga
     5461 gctcggcctg cactggcagg aaaagctgca gcgcttcatg gacgacaagg tccatccgcg
     5521 caccgccgaa tattgggccc aggtccatgc cgaccacacg gtccagccac cgatcatgga
     5581 gaccatgaag gccgaggcgc gcgaagccgg tctgtggaac atgttcctgc cgggcgatca
     5641 cggcgctgga ctgacgaacc tcgaatacgc gccgctggcc gagatcatgg gccgcgcgct
     5701 gtggtcggcc gaggtgttca actgcaacgc gcccgacacc ggcaatatgg aagtcctcca
     5761 tatgtacggc aacgaggccc aacaggcgca gtggctgaag ccgctgatgg ccgggacgat
     5821 ccgctcggcc ttcctgatga ccgagcccgc cgtcgcttcg tctgacgcga ccaacatcca
     5881 gacctcgatc gtgcgcgagg gcgacgaata cgtcatcaac ggccgcaaat ggtggtcgac
     5941 caacatgggc catcccaacg ttaagatcgc catcgtcatg ggcaagacgg acacctccgc
     6001 cgccacccac tcgcagcagt cgcaagtgct ggtgcccacc gatacccccg gcttccgtat
     6061 cgagcggatg ctgaccgtgt tcggctatga cgagcgcccg atcggccatg ccgaggtcgt
     6121 tctcgataat gtccgcgtcc cggtgaccaa cctgatcgcc ggcgagggca ggggcttcga
     6181 gatcgcgcaa ggacgtctcg ggccgggccg tatccatcac tgcatgcgca ccatcggcgc
     6241 agcggagttg gcgctggagc tgatgtgcga acgcctgctc aaccgcacgg cgttcaagaa
     6301 gcagctggcc gaacattcgg tctgggaaca gcgcgtcggc gaggcccgca ccaatatcga
     6361 gatgtgccgc ctgatggtgc tgaaggccgc ttggatgatc gatcaggccg gagccaagaa
     6421 cgccaagtcc gagatcgccc agatcaaggt cgccgcgccg aagatggccc tgcagatcat
     6481 cgacgacgcc attcaggcct tcggcggcgc gggtgtgtcc ggcgatacgc cactggccga
     6541 actctacgcc ggtgtccgca ccttgcggct cgccgacggc ccggacgagg tccacaatag
     6601 gaccatcgcc cgtctcgaat acgccaagca cggcggccgc ccagttcgat agaggtgttt
     6661 ttgtcagcag tcgttcgccg ctgggctctt tccggcggcg aacgggtcct gcccggtggc
     6721 aaagtcgcct gagtcaccta tctgttcggg atcggcgagg tctgggtccg cgtacaggaa
     6781 ccaaacttag ccggtgacat tctgaggctg ggtgggtgac cgccgtgggc gaccggggcg
     6841 gacctgacgg cgatgaacga tctcacacag attcagtcgt cccaatcgga cgacgtcgcc
     6901 ctctaccgta acgtccgcga tcgcatgatc gagctggcca agggcctctc ggccgaagac
     6961 ctggccgccc agcccatggc cgacgccagt cccggtaaat ggcatctggc ccatacgagc
     7021 tggttcttcg aggcgatgat tctggccagc gagccgaact atcaaccggt cgatccgcgc
     7081 ttccagcagc ttttcaactc ctactacgaa gccctcggcc gccgggtaga ccgccacgag
     7141 cgcggcctga tgacccgccc cggactggac gacgtcctcg cctatcgtcg cgagatcgac
     7201 cgccgcatgg tcgaccgtct gtcgcgcggc ctgacctcgg gcgccgaacg ctatctgttc
     7261 gtgctcggcc tgcatcacga ccagcagcat caggagctct tcctgatgga cctgctcaat
     7321 ctgatgtcgc gctcgccgct ggaccctgcc gcctacgatg tcgaaccgcg cgacgcccct
     7381 gtgcagccct ccgtcggcgg cgtgacccgt ttcgacggtg gtctcatcac cctcggccac
     7441 gacggcgagg gcttcgcctt cgataacgag ggcccggcgc accagacttt cctgcgcccc
     7501 ttcgctctcg ccaacgacct cgtcaccaac gccgactgga tccgcttcat cgacgacggc
     7561 ggctattcac gccccgagtt ctggctcgcg gacggctgga ccacggtcaa ggccgagcac
     7621 tggactgcgc ccctctactg gcatgcggag gagggcggct ggtccgtctt cggcctcgct
     7681 ggcgcggctc ccgtcgaccc gtcagcgcca gtccgccacg tcagtttcta cgaggccgac
     7741 gcctacgccc gctgggccgg caaacgcctg ccgaccgaag ccgagtggga atacgccgcc
     7801 gtcacagacg ccgcgccctt ttcgaacctg ttcggcgaag tctggcagca caccggcagc
     7861 gcctacaccg cctatcccgg cttccgcccg accgacggca cggcgtccga gtacaacggc
     7921 aagttcatgg ccaatcagat ggtcctgcgc ggctcctgtt tcgccacgcc gctgggccac
     7981 acccgcgcga cctaccgtaa tttcttttat ccgcatcaaa gatgggcgtt catgggcctc
     8041 cggcttgccg aagacatcgc cgccgaccaa tcaacggttg acgaagccgg tcacgataag
     8101 gggggcgaga ccgccgcttt ccgcgccgaa ttcatcgccg gcctctcgca gccgcagaag
     8161 gctgtttcgc cgaaatggtt ctacgacgcc gaaggctccc gcctgttcga ggacatcacg
     8221 cgtctgccgg aatactatcc cacgcgacaa gaagcctcgc tgctcaaggc gctcgccccg
     8281 cgcctgaccg aggatttcgg caacgacgcc gtgctcgtcg agttcggctc aggcgccagc
     8341 gagaagaccc gcatcctgct cgacgccgcc ccgacgctgt ccacctacat tcccatcgac
     8401 atcagccccg atgcgctgaa cgaggcggcg gagcggatca ccgcagccta tccgtccatc
     8461 gaggtcgtgc ccgtcgtcgg cgattttctg aacctgccgt cgctgcccga actgggccgc
     8521 ggccgccgta tcggcttctt ccccggctcc accatcggca acctcgatcg tgacgctgcg
     8581 gtggagttcc tgaaggtcgc acgcgctcgc ctcgacaccg gctcgctgtt cattctcggc
     8641 gtcgatctgg tcaaggcgcc cgagctcctg atcgccgcct atgacgacgc gcaaggcgtc
     8701 acggcggcgt tcaacaagaa cctgctggtc cgcgccaatg ccgagctcgg caccgatttc
     8761 gatctcgacg ctttcgagca tctggcgctc tggaatgagc cggaatcgcg gatggagatg
     8821 caccttcggt cggcccgcga ccagcgtgtg accatcggcg acaccgtgat ccggttcgta
     8881 aagggtgaga ccatccacac cgaaagctcg cgcaagtaca ctcgcgcgtc ggtgatcacc
     8941 ctcgccgaag ccgccggctg gaccgtctcg ctgttcgaga gaagcgcgga gccgtcggtc
     9001 gcactggtgc tgttgagcgc ctgaggcgcc cgcccgggcc gcgacggaca aagaaaaagc
     9061 ggcgaaacca gatctggtct cgccgctttt tcgtctgcgc ttgagcggag cctattcggc
     9121 ctggccttcg acgtcttcag gacgccgcga gccgtgcgaa gcctcgccgc ccttgcgtcc
     9181 ggcctgcgcc gccaatccgc gatcctgtga gaagctgcgc ttctcgctgg ggacgcttgc
     9241 tccacccttg cgggcaattt cgcgctgccg ttcggggtcc atggaggcaa agcctctttt
     9301 ggatacaccc gatgggcggt tgggctgacc ttctgccatt cggctgttcc ttcctgagta
     9361 gtgttggtta ttgcagtgaa caaccccggc aatcggccaa ggttccgtgc gcggatcgtc
     9421 cgttaaccag aacgttcaga tcgctttcgc gtcgaacgtt cgcgaatcac cttcgtcacc
     9481 accacccggc tgcgccgggg gcatctcgtc gggcacgcca gcgggatcga tgtcgggttg
     9541 atcgttctgg ggttctggaa attcagcggg ctggccggga tcgatgtagg tcatgttctt
     9601 ctcctgttcg ggaagccaac gtctctgcgg ccgctccgtt cagacaagag ttcgtcactg
     9661 gcgacaccga gccggaaccg gaaagcgggc gggcggttgt tcgaaccgcg ccccgctcaa
     9721 aggatattcc catgaccgac acctattcga cgaaccccga cgacgtcctg tctgaagacc
     9781 tgcaggacga aaccccgtcc gcccgtctcg accgtcaggc cgacgccctt ctcaacgacg
     9841 ccgacgatcg ggcctttgcg cgctcggaag gcatccgcaa agccgtccgt tctgacctcg
     9901 aacacggcaa gcagtgggct gccgaccgaa cggagatgac ccgcgacgct atccgtgacg
     9961 agcctatgaa gaccgctctc tatgctgtcg gtatcggcgt tctcatcggc ctgcttctgc
    10021 ggcgctgagc gctcaggagg ggccgctcgc ctcgcgttcg agccgcggcc ccttcatcgc
    10081 ccgatccgac acaaacgggt tggtctgccg ttcctgtccg aatgtagaga ccggtccgtg
    10141 cccgggcacg aaagtcacgc catctccgag cggccaaagc ttttcggtga tcgaccggat
    10201 cagggcatcg ggatcgctac gggggaaatc tgtccgccca acggaccctt ggaacagcac
    10261 gtcgccgacc tgcgcaaacc gcgcctcccg attaaagaat atgacatgac ccggagtatg
    10321 ccccgggcag tgcagcacct cccaggtggt ctcgcccagc gtcaccgtat caccgtcctc
    10381 cagccagcgg tctggcttga acgaccgcgc atcggggatc ccgtacatct ggccctgcgc
    10441 cggtatgccg tcgatccaga actgatcctc gagctgcgga cccgtgatcg tcacgccgga
    10501 cttctcctgc atctccgccg cgcccccggc atgatccaga tgtccatggg tgatccatat
    10561 ctgatccaga gtcaggccgc gccgcgccac ctcgctgagg atcgcgtcca ccgacccgcc
    10621 cggatcgatc acggcggcct tcatggttct ggtgcaccac acggtcgtgc agttctgctg
    10681 cagcggcgtg accgccgtta tgaagacccc aatgggtggg ggagggacgg gcgactggct
    10741 catgcatgaa agatagcggg cagacgcccc gggtgaaagc ggctgggtat cttctccctg
    10801 tctactgcgg cgaccacgtg cgcgttgacg tttgaagtcc atctcgacat aagggcgggc
    10861 ttcgaggccg ccctgaaaat gggcggctct tctgctttat ccattccgcg catcgccagt
    10921 tcggcccgat gcgccccaga acgtcagatc ccgaccatgc aagtcgtcga aaaaaccacc
//
LOCUS       DASVTM010000209         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_233799,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000209 DASVTM010000000
VERSION     DASVTM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233799"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF633_05645"
     CDS             <1..234
                     /locus_tag="VF633_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470576.1"
                     /translation="DIGLPDGSGLELLSELNDEAGRAVPVIIYSAQEMDAALAGRVEA
                     VLTKSRTSLAGLARTVRRLTNARKTDEAPSIEP"
     gene            231..623
                     /locus_tag="VF633_05650"
     CDS             231..623
                     /locus_tag="VF633_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470577.1"
                     /translation="MKILHIDDEPDIREITALSLGIDPDIELTSCPSGRTALDALEGG
                     LRPDAILLDVMMPGLDGPGTLLQVRTIAGLTETPVIFMTARAQAQEQARFIGLGAIGV
                     IIKPFDPMTLAGQVRDILAAAGPKVPRR"
     gene            complement(901..2259)
                     /locus_tag="VF633_05655"
     CDS             complement(901..2259)
                     /locus_tag="VF633_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8470578.1"
                     /translation="MQIPDTAVTTPHAKKPFYTHLYFQVLTAIFLGAVIGHFWPEFGE
                     SLKPLGDAFIKLVKMVIAPVIFLTIVTGIAGMRDLGKVGRVAAKAFAYFLTFSTLALI
                     LGLIVANVVQPGRGLNIDPATLDAGAVAQYSSAAHETTMQGFLMGIIPDTVVSAFSTG
                     NILQVLFVSILFGIALAMIGDVGKPVLTFLESVSAVFFKLVAILMKAAPIGAFGAFAF
                     TIGAYGIGSIANLLALVATFYLTAILFVVGVLGLVGIANGFNILKLIRYLKEELLLVL
                     GTSSSEAALPSLLQKMERAGAAKSVVGLVVPTGYSFNLDGTNIYMTMAALFIAQALNI
                     HLSLEQQVLLLAVAMLSSKGAAGITGAGFITLAATLAVVPTVPIAGMALILGVDRFMS
                     ECRALTNFIGNAVATIVVARWENQLDRDALKAALDGHPLPLAAYPDPAAGPGDKAVLA
                     DD"
BASE COUNT          467 a          764 c          778 g          388 t
ORIGIN      
        1 gacatcggcc tgcctgatgg atccggtctg gaattgttga gcgagctgaa cgacgaggca
       61 ggtcgagcgg tgccggtcat aatctattca gctcaggaaa tggacgcggc cctcgccgga
      121 agggtcgagg ccgtgctgac gaagtcccgc acgtcgctgg ccggcctggc gcgcaccgtg
      181 cggcgtctga ccaacgccag aaagaccgat gaagcaccga gcatcgaacc atgaaaatcc
      241 tgcatatcga cgacgagccc gacatccgcg agattactgc tctctccctc ggcatcgacc
      301 ccgacataga actgacctcc tgtccgtcgg gtcgaacggc gctggacgcg cttgaaggcg
      361 ggcttcgacc cgacgccatc ctgctggacg tgatgatgcc tggcctcgac ggcccaggca
      421 cgctgttaca ggtccggacg atcgcggggc tgactgagac gccggtcatc ttcatgaccg
      481 cccgcgccca ggcccaggaa caggcccgct ttattggcct gggagccatc ggggtaatta
      541 tcaagccatt cgaccctatg accctcgccg gccaggtgcg ggacatcctg gcggcagccg
      601 ggccgaaggt cccccgcaga tgatcgatcc tctggcggcg ctgcgagaca agtttcgcgc
      661 ccggtccgcg gacgacctca cgcggctgag gatgctgatt cagggcgatt tgtcgtcgac
      721 ggaactgcgc catctggccc atagcctggc aggctccgca gggacgttcg gttttccgac
      781 gctgagcgcg gccgcggcgt cagtcgacga cgactacgcg ctcggccgca cccctgatcg
      841 gtcttccttc gacaggctgg agcgcgaact ggaacagctg gcgacgccca cgagccgagt
      901 tcagtcgtcg gcgaggacag ccttgtcacc gggtccggcg gctgggtctg gataggcggc
      961 gagcggcagg ggatggccgt cgagggcggc tttcaaggcg tcgcggtcga gctgattttc
     1021 ccatcgagcc acgacgatcg tggccacggc gttgccgatg aagttggtca gggcccggca
     1081 ctcgctcatg aaccggtcca cgccgaggat cagcgccatg cccgcgatcg gcacggtcgg
     1141 caccaccgcc agcgtcgccg ccagggtgat gaagcccgcg cccgtgatgc ccgcagcgcc
     1201 cttggacgac agcatggcca ccgccagcag cagcacctgt tgctcaaggc tgaggtgaat
     1261 gttcagggct tgcgcaatga acagcgcggc catggtcatg tagatgttgg tgccgtcgag
     1321 gttgaacgaa tagcccgtcg gcacgaccaa cccgactacc gacttggcgg ccccggcgcg
     1381 ctccatcttc tgcagcaggc tcggcagggc ggcctcggag gacgaggtgc cgagcaccag
     1441 cagcagctct tccttcaggt agcggatcag cttgaggatg ttgaagccgt tggcgatccc
     1501 taccagcccc agcaccccga ccacgaacag gatcgcggtc agatagaagg tcgccaccag
     1561 cgccaacaga ttggcgatcg agccgatgcc ataggcgccg atggtgaagg cgaaggcccc
     1621 gaacgccccg atcggcgctg ccttcatcag aatggccacc agcttgaaga agacggccga
     1681 gaccgactcg aggaaggtca gaaccggctt gccgacatcg ccgatcatgg ccagggcgat
     1741 gccgaacagg atcgagacga acaggacctg caggatgttg ccggtggaga aggcgctgac
     1801 caccgtgtcg gggatgatcc ccatcaggaa gccctgcatc gtcgtttcgt gcgcggcgct
     1861 cgaatactgg gcgactgcgc cggcatccag cgtcgccgga tctatgttca ggccccggcc
     1921 gggctggacc acattggcga cgatgaggcc caggatcagc gccagcgtcg agaaggtcag
     1981 gaaataggcg aaggccttgg ccgccacccg tccgaccttg cccagatcgc gcatcccggc
     2041 gatgccggtg acgatggtga ggaagatcac cggggcgatg accatcttca ccagcttgat
     2101 gaaggcgtcg ccaagcggct tcaggctctc gccgaactca ggccagaaat ggccgatcac
     2161 cgcaccgagg aagatcgccg tcagcacctg aaaatacagg tgggtgtaga agggcttttt
     2221 cgcatgcggc gtcgtcacgg ccgtgtcggg tatctgcaag cgtcgcatcc ttctgaatcg
     2281 tcgagactat cgtaaaggat caacccggtc ggtggtcaaa aaattgatcc gcgataacga
     2341 cgcagcgcct tagcccggat cgccgtcgcg gaaccgccac ttcatgatca ggacgcc
//
LOCUS       DASVTM010000210         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_924953,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000210 DASVTM010000000
VERSION     DASVTM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924953"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF633_05660"
     CDS             <1..249
                     /locus_tag="VF633_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8470579.1"
                     /translation="RNGTEVHLGPTEFRLLDHFMRHPGRVFSREQLLDAVWGSDVYVE
                     ARTVDVHVGRLRKALDIGESANPIRTVRSAGYSLDLEG"
     gene            complement(258..1196)
                     /locus_tag="VF633_05665"
     CDS             complement(258..1196)
                     /locus_tag="VF633_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HEX8470580.1"
                     /translation="MPHSAPAATTPIAPTDDLAAAFQIEGWPVRGRLVRLGEAVDTII
                     KAHDYPEPVAALLGEACALAALVGSSLKFNGRLIVQAQGDGPVRYVVADYDTSGTLRG
                     YCRFDEAEVAAASTGFARPGAQSLLGKGVFIMTLDQGPDFDRHQGITPIEGESLSLCA
                     EHYFAQSEQVPTKVHLAVGQVSSDAGMVWRAGGAMIQMVAGDETRGSTQEVWDRTRAL
                     FQTLADDELIDPTITPEILLFRLFHEDGVRLEQPLALSAQCRCSKERIVGVLGSFSAD
                     ERADMVEDDGKIRVTCEYCSTIYALDPAEVASAAAG"
     gene            1349..>2576
                     /locus_tag="VF633_05670"
     CDS             1349..>2576
                     /locus_tag="VF633_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8470581.1"
                     /translation="MAKPTLKPLDQQAIVITGATSGIGLATARRAARGGACVFMIARG
                     EKDLRALCEELQGTGARVAWAAADVADYDALAEAADKCRRLFGGFDTWINNAGVSIIG
                     SVLGTTLEDQRRLFETNYWGVVNGSTIAAEHLRGRPGGGAIINVGSTLSDAPSPIRGA
                     YSASKHAVKGFTNALRMELLRDKAPISLSLVKPAAVDSLYDHHARNLAGYAAQNPQPV
                     YATHVVADAILWCATHPMREITVGGGGRLIASFYAALPGIAEPLFARFAPRLKRDSGP
                     GYEPYEDGLYDASDDGLQEEVYYPMVRQFSVLAEARKHPGITAGSVALVAVAAIATVL
                     LSQRTGPTRYRELRDRLDPRGWIDAEALRHRFEDISRGLSDTAGDLGHRAEDFTGEAR
                     SRAGKFGEEARHHAGKL"
BASE COUNT          434 a          838 c          886 g          418 t
ORIGIN      
        1 cgcaacggca ccgaggtcca cctgggtccg accgagttcc gcctgctgga tcacttcatg
       61 cgtcacccgg gccgggtgtt cagccgcgaa caattgctcg acgccgtctg gggctccgac
      121 gtctacgtcg aggcgcgcac cgtcgacgtc cacgtcggcc gcctccgcaa ggcgctcgac
      181 atcggcgaaa gcgccaaccc catccgcacg gtcagatccg ccggctattc gctggatctg
      241 gaggggtagg gcggccgcta ccccgcggcc gcactcgcca cctcggccgg gtccaatgcg
      301 tagatcgtcg agcagtattc gcaggtgacg cggatcttgc cgtcgtcctc aaccatgtcg
      361 gcgcgttcgt cggccgagaa cgatcccagc acgccgacga tgcgctcttt cgagcagcgg
      421 cactgggctg acagcgccag cggttgctcg agccgcacgc cgtcctcgtg gaacaggcgg
      481 aacagcagga tttccggcgt gatggtcggg tcgatgagct catcgtcggc cagcgtctgg
      541 aacagggcgc gcgtgcgatc ccagacttcc tgcgttgagc cgcgcgtttc gtcgccagcg
      601 accatctgga tcatcgcacc ccctgcgcgc cagaccatgc ccgcgtcgct ggaaacctga
      661 ccgacggcca gatgcacctt cgtcggcacc tgttcggact gggcgaaata gtgctcggcg
      721 cacagcgaca ggctttcgcc ctcgataggg gtgatgccct ggtggcggtc gaagtcgggg
      781 ccttggtcca gggtcatgat gaacacgccc ttgcccagca ggctttgcgc gccggggcgg
      841 gcgaaaccgg tggaggctgc ggcaacctcg gcctcgtcaa agcggcaata gccacgcagg
      901 gtgcccgagg tgtcgtagtc ggcgacgacg tagcggaccg gaccgtcgcc ctgtgcctga
      961 acgatcaggc ggccattgaa cttcaggctc gagcccacca gcgcggccag cgcgcaggct
     1021 tcgcccagca acgccgcgac gggctcggga tagtcgtggg ccttgatgat ggtgtcgaca
     1081 gcctcgccca atcggaccag acggccgcgc acgggccagc cttcgatctg gaaggcggcg
     1141 gcgaggtcgt ccgtcggagc gataggcgta gtggcggcag gcgcggaatg aggcactggc
     1201 tcgggtcccg aaatcgaatg tctggaaggg cgacaagata gggaccggat gtcgccaagg
     1261 taagggggct gtgatctaac ggcggaaccc ggccttctgg cgacccgttc cagcggggct
     1321 ggagacgggc gtagcaacga ggcgagacat ggcgaaaccg acccttaaac cgctggacca
     1381 gcaggccatc gtcatcaccg gcgcgacctc gggcatcggc ctggccacgg cccgacgtgc
     1441 ggcgcgaggc ggagcctgcg tcttcatgat cgctcgcggc gagaaggacc tgcgcgccct
     1501 ctgtgaggag cttcagggga cgggagcgcg cgtcgcctgg gccgctgcgg acgtggcgga
     1561 ctatgacgcg ctcgctgagg cggcggacaa gtgccgtcgg ctgttcggtg gcttcgacac
     1621 ctggatcaac aacgcgggcg tctcgatcat aggcagcgtg ctggggacga cactggagga
     1681 tcagcgccgg ctgttcgaga caaactactg gggcgtggtc aacggctcga ccatcgcggc
     1741 ggagcatctg cgcggaagac cgggcggtgg cgcgatcatc aatgtcggct cgacattatc
     1801 tgacgcgccg tccccgatcc ggggcgcgta ttcggcgtcg aagcatgcgg tgaagggctt
     1861 caccaacgcc ctgcgaatgg agttgctgcg agacaaggcc ccgatctcgc tcagtctggt
     1921 caagccggcg gcggtggact cgctctacga tcaccatgcc cgcaacctcg ccggatatgc
     1981 ggcccagaac cctcagccgg tctatgcgac ccacgtcgtg gccgacgcca ttctgtggtg
     2041 cgcgacccat ccgatgcgcg agatcaccgt cggcggggga gggcggttga tcgcgtcttt
     2101 ctacgcagct ttgccgggga tcgcggaacc actcttcgcg cggttcgctc ctaggctgaa
     2161 gcgggacagc ggtcccgggt atgagccgta tgaggatggt ctgtacgacg cgtccgacga
     2221 cggcctccag gaagaggttt attatcccat ggttcgacag ttcagcgttt tggcggaagc
     2281 ccgcaaacat cccggcatca cagcgggctc ggtcgcgctc gtcgctgtcg cggccatcgc
     2341 caccgtactg ttgagccagc gcaccgggcc gacccgctat cgcgagcttc gcgatcgtct
     2401 cgacccacgc ggctggatcg acgccgaggc cctgcgccat aggttcgagg acatctcgcg
     2461 cggtctgagc gataccgccg gggacttggg ccatcgcgcc gaggacttca ccggcgaagc
     2521 ccgcagccgt gccggcaagt tcggtgaaga ggcgcgtcat cacgccggga agctgt
//
LOCUS       DASVTM010000211         5970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_869943,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000211 DASVTM010000000
VERSION     DASVTM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869943"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VF633_05675"
     CDS             complement(<1..121)
                     /locus_tag="VF633_05675"
                     /inference="COORDINATES: protein motif:HMM:NF045295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470582.1"
                     /translation="MTVPGQPLSDGERSARLRLARADRIGPVTFHQLLQRFGSA"
     gene            complement(118..756)
                     /gene="plsY"
                     /locus_tag="VF633_05680"
     CDS             complement(118..756)
                     /gene="plsY"
                     /locus_tag="VF633_05680"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX8470583.1"
                     /translation="MQDLVAPALGSLALMAIGGYLLGSIPFGVVITRAAGAGDVRNIG
                     SGNIGATNVLRTGRKDLALATLLLDGAKGAVALLIARWLFDSEVAGAIAGGAAFFGHL
                     FPVWLKFKGGKGVATFFGLLIAAHWPLGLMAGATWLIVAFVLRYSSLAALIAAVLAPV
                     YALLPLPGAGLPVAQPILILSIVTAVLIWIRHHENIGRLLKGAEPRLGAKKT"
     gene            902..1816
                     /locus_tag="VF633_05685"
     CDS             902..1816
                     /locus_tag="VF633_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX8470584.1"
                     /translation="MIRPVLLAAASLFVLTGCAGTPQIASSGPRLAVWFDCVRDGGVV
                     ISAHRGQSGEDQPENSLAAIRATGRAIPNAIVELDAVLTSDGKLTLMHDDTMDRTTTG
                     QGKVSDLTLAQVKAARLRAPDGHLTNEAPPTLAEALAAAGEVHAIASIDLKPASEAAT
                     LTLARAVIDEVRRVGAADRVILITYSPETARAVADMAPEMMISAGVKSIDELEGLRRP
                     QILAWTGAGRPNPELWSAMKAVGVEAQFGTLGGSDRALDRVYAADGNVSEYRDLIAQG
                     VTVIATDEPLAVKSVLGDAVAKAASCKR"
     gene            complement(1849..3126)
                     /gene="pyrC"
                     /locus_tag="VF633_05690"
     CDS             complement(1849..3126)
                     /gene="pyrC"
                     /locus_tag="VF633_05690"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX8470585.1"
                     /translation="MSALAFINVRLLDPATDYDGPGSVLIEDGRIVEVVRGGAAQVPA
                     GVEVIDANGKCLAPGLIDIRVKTGEPGSEPKETLKSASLSAAAGGVTTIVIQPDTDPA
                     VDDPAMVDFIQRRGAALNLVNVLVAGAATRNRDGQRMAEIGLMDEAGAVYFTDGDRVI
                     ANSRTLQRVMSYAAAFNALIACRPTDPWLSEGAVATSGELATRLGLSSAPAIAERIQL
                     ERDLALVEQTGARFLVDQISTTGALETLARARAKDLEVAASVSINHLCFNEIDIGDYR
                     TFFRLDPPLRPESDRQALIEAVRDGLIDVITSAHAPAPAEDKRRPFGEAAPGAVGLET
                     LLSASLMLHHEDGLELLDILRPLTHGPASLLGLASGVLAEGAPADLVLFDAHAPVVID
                     AAMLKSKSKNSPFDGRRLQGKVLGTWVAGRKIY"
     gene            complement(3123..4103)
                     /locus_tag="VF633_05695"
     CDS             complement(3123..4103)
                     /locus_tag="VF633_05695"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX8470586.1"
                     /translation="MTDSATVTDLISERLVPFPSAHFLSAGDLNPPATLALLDLADTF
                     VDFNRQSSKSLDLMHGRTVVNIFFENSTRTSSSFEIAAKRLGADVVTMPVQASSVAKG
                     ETLIDTAVTLNAMKPDILVIRHASSGAAALLSQKVGCAVVNAGDGRHEHPTQALLDML
                     SLRRAFGDVGGLTVAICGDIAHSRVARSNVILLSMMGARVRLIGPPTLVPGDADRWGC
                     EVFHDMREGLQGCDVVMMLRLQLERMEGALVPSTREYFRFWGLDREKLAWAMPNAKVM
                     HPGPMNRGVEIDSDVADDLSVSLIQDQVEMGVAARMAVLASLATRRGDDR"
     gene            complement(4127..5056)
                     /locus_tag="VF633_05700"
     CDS             complement(4127..5056)
                     /locus_tag="VF633_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX8470587.1"
                     /translation="MSFAALISGVVPVFVMIALGYGLKKSNFLPDATWRPIEKLSINI
                     LYPGFLIPAIWGADLGSGSAGAAAGAAVVAVLIVSAATLLAKPLMKIDGPAYTSVYQG
                     VIRWNSFVFLPVIQASFGTEGLALAAVMIACIIPVTNIGCVAVLARWGADQRGVTPLA
                     LLKAMLANPILLACLIGLTLNLLGVPGIPGVTDTLKLLGSAALPLGLIIAGAGLSFAE
                     VARRKWTIAGTSFVKLIIMPPLMWGLCILFGGDHLAQGVAMLCGAAPGAAASYLLARQ
                     MGGDAPLMAGVIALTTVTSAAMIPLLLFIYHLT"
     gene            complement(5062..5541)
                     /gene="ruvX"
                     /locus_tag="VF633_05705"
     CDS             complement(5062..5541)
                     /gene="ruvX"
                     /locus_tag="VF633_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX8470588.1"
                     /translation="MPVLDLLDLPAACPPGTPWLGLDLGENTIGVAASDTSRMIASPL
                     ELIRKEKFSLDAARLIKLMNGRNVSALVIGLPINMDGSEGPRAQSCRAFARNLERLRP
                     VNVAFWDERLSTSAVERFLIDELDLSRKRRAGVIDRTAAAWILQGALDRVRDQATWI"
     gene            5659..>5970
                     /locus_tag="VF633_05710"
     CDS             5659..>5970
                     /locus_tag="VF633_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470589.1"
                     /translation="MNQKLLTAAVLMGALALSGCDRLGAAWTAFQAPKAPVGAVPVAP
                     PAPAAAPVLAPLTPTSRGLPVKAVPDPELQSPASVVEVHPLTSVTGMDGKIFGLAGGD
                     PA"
BASE COUNT         1034 a         2055 c         1968 g          913 t
ORIGIN      
        1 cggcgctgcc gaaccgctgg agcaactgat ggaaggtcac ggggccgata cggtccgcac
       61 gcgcgaggcg cagacgggct gaccgctctc cgtcggaaag cggttggccc gggacggtca
      121 agttttcttg gcgcctaagc ggggctcggc gcccttgagc aggcggccga tgttctcatg
      181 gtggcggatc cagatcagca ccgccgtgac gatcgacagg atcaggatcg gctgcgcaac
      241 cggcaacccc gctccgggca acggcagcag cgcatagacc ggcgccagca cggcggcgat
      301 cagggcggcg agcgaggaat acctcagcac gaacgccacg atcagccagg tcgccccggc
      361 catcagcccc agaggccaat gcgcggcgat cagcaggccg aagaaggtcg cgacgccctt
      421 gccacccttg aatttgagcc agaccgggaa caggtggccg aagaaggccg cgccgcccgc
      481 gatggctccg gctacttcgc tgtcgaacag ccagcgggcg atcagcagag ccacggcgcc
      541 cttggccccg tcgagcagca gggtggccag cgccagatcc ttgcgcccgg tccgcagcac
      601 attggtcgcg ccgatattgc cagagccgat gttgcgcacg tctccggcgc ctgcggcacg
      661 ggtgatgacc acgccgaacg ggatcgaacc cagcagataa ccaccgatcg ccatcagggc
      721 gagcgagccc aacgccggcg ccacaagatc ctgcaactga ttcgctcccc gtccctgatt
      781 tcgcggcacg atgcgtcgcg ggccgagcgc gagcaagcgt ccgatcatct tgggttgtca
      841 aacggtgtct tgacctcggc gggtgttgcc gatttggtcc gcccgcttct ggagttcccg
      901 catgatccgt cccgtccttc tcgccgccgc atccctgttc gtcctgacag gctgcgcggg
      961 tacgccgcag atagcttcat cgggcccgcg gctggccgta tggtttgact gcgtccgtga
     1021 cggcggcgtg gtcatctcgg cgcacagggg tcagtctggc gaggaccagc ccgagaactc
     1081 gctggccgcc atccgcgcca ccggccgcgc catccccaac gccatcgtcg agctcgacgc
     1141 cgtcctgacg agcgacggca agctgaccct gatgcacgac gacaccatgg accgcaccac
     1201 gaccggtcag ggcaaggtct ccgacctgac gctggcccag gtcaaggcgg cccggttgcg
     1261 cgcgcccgac ggccatttga ccaacgaagc cccgcccaca ctggccgagg cgctcgccgc
     1321 cgccggtgaa gtccacgcca tcgccagcat cgacctgaag cctgcgtccg aagctgcaac
     1381 cctgacgctg gcccgcgccg tcatcgacga ggtccgccgc gtcggcgccg ccgaccgcgt
     1441 catcctgatc acctacagcc ccgaaaccgc ccgcgccgtc gccgacatgg cccccgagat
     1501 gatgatctcc gccggggtga aatcgatcga tgaactggag ggcctgcgcc gccctcagat
     1561 cctcgcctgg accggcgccg gtcggccgaa cccagagctg tggagcgcca tgaaggccgt
     1621 cggtgtcgaa gcccagttcg gcacgcttgg cgggtcggat cgcgcgctgg atcgggtata
     1681 tgcggcggac ggcaatgtca gcgaataccg cgatctcatc gcgcagggcg tgacggtcat
     1741 cgccacggat gaaccgctgg cggttaagag cgtgctgggt gatgcggtgg cgaaggcggc
     1801 gagttgtaag cggtagcttc cctctcccgg cgggagaggg aagagaagct agtaaatctt
     1861 cctacccgcc acccaggtcc ccagcacctt gccctgcagt ctgcgcccgt cgaacggtga
     1921 gttcttggac ttggatttca gcatcgcggc gtcgatcacg accggcgcat gggcgtcgaa
     1981 cagcaccaga tcggccggcg caccctcggc cagcacgccc gaggccagcc ccaacagcga
     2041 cgccgggcca tgcgtcagcg ggcgaaggat atccagaagc tcaagcccgt cctcgtggtg
     2101 cagcatcaac gatgccgaca gcagcgtttc caatcccacg gcgcccggcg cggcctcgcc
     2161 aaacggacgg cgtttgtctt cggcgggggc aggggcgtgg gccgaggtga tgacgtcgat
     2221 caggccatcg cggaccgcct cgatcagcgc ctgacggtcg ctctcgggtc gcagcggcgg
     2281 gtcgagccgg aagaaggtcc ggtagtcgcc gatatcgatc tcgttgaagc acaggtggtt
     2341 gatcgagacc gaggccgcga cttccagatc cttggcccgc gcccgcgcca gcgtctcaag
     2401 cgcacccgtc gtcgagatct ggtcgaccag gaaccgcgcg ccggtctgct cgaccaaggc
     2461 cagatcccgc tccagctgga tgcgttcggc gatcgccgga gcgctcgaca gtcccagacg
     2521 cgtggccagt tcgcccgacg tcgcgactgc gccttcgctc agccacggat cggtcggacg
     2581 acaggcgatc agggcgttga aggcggcggc atagctcatc acccgctgca gggtgcggct
     2641 gttggcgatg acgcggtcgc cgtcggtgaa atagaccgcg cccgcctcgt ccatcagacc
     2701 gatctcggcc atccgctggc cgtcgcggtt gcgtgtggcc gcgcccgcga ccagcacgtt
     2761 gacgaggttc agcgccgcgc cgcgccgctg gatgaagtcg accatggccg ggtcgtccac
     2821 ggcggggtcg gtgtcgggct ggatgacgat ggtggtgacg ccaccggccg ccgccgacag
     2881 gctggccgat ttcagcgtct ccttgggctc ggaccccggc tcgccggtct tgacccggat
     2941 gtcgatcagc ccgggggcga ggcatttgcc gttcgcgtcg atgacctcga cgcccgcagg
     3001 aacctgcgcc gcgccgccgc gcacgacctc aacgatgcgg ccgtcctcga tcaggaccga
     3061 gcccgggccg tcatagtcag tcgccggatc gagcagccgc acgttgataa aagcgagcgc
     3121 actcatcggt cgtctccccg acgggtcgcc agcgaggcca gcaccgccat gcgtgcggcg
     3181 acgcccatct cgacctgatc ctgtatcagc gacaccgaca ggtcgtcggc aacgtcggaa
     3241 tcgatctcga ccccccggtt catcggcccc gggtgcatga ccttggcgtt gggcattgcc
     3301 caggccagct tctcgcggtc cagaccccag aagcggaaat actcgcgggt cgaaggcacc
     3361 agcgccccct ccatccgctc gagctgcagc cgcagcatca tcacgacgtc gcagccttgc
     3421 aggccttcgc gcatgtcgtg gaacacctca cagccccagc ggtcggcgtc gcccggcacc
     3481 agcgtcggcg gtccgataag ccgcactcgc gcgcccatca tcgacagcag gatgacgttg
     3541 gaccgcgcca cccggctgtg ggcgatatcg ccgcagatgg ccaccgtcag cccgcccacg
     3601 tccccgaagg cgcggcgtag ggacagcatg tccagcaagg cttgcgtggg gtgttcatgg
     3661 cgtccgtcgc cggcgttgac gaccgcgcag ccgacctttt gcgatagcag ggcggcggcg
     3721 ccggacgagg catggcggat caccagaatg tccggcttca tggcgttcag cgtcaccgcc
     3781 gtgtcgatca gcgtctcgcc cttggccacc gaagacgcct gcaccggcat ggtcacgacg
     3841 tccgcgccga ggcgtttggc ggcgatctcg aacgacgagg aggtgcgggt cgagttctcg
     3901 aagaagatgt tcacgaccgt ccgcccgtgc atcaggtcga gcgatttgga ggactgcctg
     3961 ttgaagtcga cgaaggtgtc ggcgaggtcc agcagggcca gcgtcgcggg cgggttgaga
     4021 tcgccggcgg acaggaaatg ggcggacggg aacgggacca gtcgttcgga gatcagatcg
     4081 gtgacggtcg ctgaatcggt catcgaggcc cgccgataaa gcggtcctag gtcagatgat
     4141 agatgaacag caagagcggg atcatcgcgg cgctggtcac cgtcgtcagc gcaatcaccc
     4201 cggccatcag gggcgcatcc ccgcccatct gccgcgccag caggtatgac gccgccgcac
     4261 ccggcgccgc gccgcacagc atggccacgc cttgcgccag atgatcgccg ccgaacagaa
     4321 tgcacagccc ccacatcagc ggcggcatga tgatcagctt cacgaacgaa gttccggcga
     4381 tggtccattt ccgccgcgcc acctcggcga agctcagccc cgccccggcg atgatcaggc
     4441 ccagcggcaa ggcggcggac ccaagcagtt ttagggtatc tgtcacgccc gggatgcccg
     4501 gcacgcccag caggttcagc gtcagcccga tcagacaggc cagcaggatc ggattggcca
     4561 gcatcgcctt gagcagcgcc agcggcgtta cgcccctctg atccgccccc cagcgcgcca
     4621 gaacggcgac gcagccgatg ttggtcaccg ggatgatgca ggcgatcatc accgccgcca
     4681 gcgccagccc ttcggtcccg aacgaggcct ggatcaccgg cagaaagaca aaactgttcc
     4741 agcgaatgac gccttgatag acggaggtat aggcgggccc gtcgatcttc atcagcggct
     4801 tggccagaag cgttgcggcg gacacgatca gcaccgcgac gactgccgct cccgccgctg
     4861 cgcccgcgct gccgctgccg agatccgctc cccagatcgc ggggatcagg aagccgggat
     4921 agagaatgtt gatcgacagc ttctcgatcg gccgccatgt cgcgtcaggc aggaagttcg
     4981 acttcttcag tccatacccg agggcaatca tgacgaagac cgggacgacg cccgagatca
     5041 aagcagcgaa actcacttaa atcagatcca cgttgcctgg tcgcgcacgc ggtcgagcgc
     5101 gccctgcagg atccacgcgg cggcggtccg gtcgatcacg cccgcacgcc gtttgcggct
     5161 cagatccagt tcgtcgatca ggaaccgttc gaccgcgctg gtcgacagcc gctcgtccca
     5221 gaaggcgacg ttgacggggc gcagccgctc cagatttcgg gcgaaggcgc ggcacgactg
     5281 ggcgcgcggc ccctcgctac cgtccatgtt aatcggcagg ccgatcacca gcgccgagac
     5341 gttccgcccg ttcatcagct tgatcagccg tgcggcgtcc aaactgaact tctccttgcg
     5401 gatcagctcc agcggactgg cgatcatgcg cgaggtgtcc gacgcggcca ccccgatggt
     5461 gttttcgcca agatccagcc ccagccacgg cgttccgggc gggcaggcgg caggcaggtc
     5521 gagaaggtcg agaacgggca ctggcgctgc atagggcagc cggctgcgga tgtcgaagga
     5581 tcgtgttgga gtgcgtaacg ccgggtcgcc attggcaaac ccgcgttagg cgttcacggt
     5641 agcgcttggg ggaatatcat gaaccagaaa ctgctcaccg ccgccgtgtt gatgggcgcc
     5701 ctggcgctgt ccggctgcga tcgtctcggc gctgcgtgga cggccttcca ggcgccgaaa
     5761 gcgcctgtgg gtgcggttcc ggttgcacct ccagccccgg ccgccgctcc cgtgctcgca
     5821 cccttgacgc cgaccagccg cggattgccg gtcaaagccg tgcctgatcc tgagcttcag
     5881 tcgccggctt ctgtggtcga ggtgcatccg ctgacatcgg tcaccggcat ggacggcaag
     5941 atcttcggac tggcgggcgg tgatccggcc
//
LOCUS       DASVTM010000212         4001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_605889,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000212 DASVTM010000000
VERSION     DASVTM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605889"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF633_05715"
     CDS             <1..366
                     /locus_tag="VF633_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX8470590.1"
                     /translation="PVLSPIVLFLAVLLISDKANAFAALGAMLMGVIVTGLFVAISMT
                     SGGGAWDNAKKVIEEGFTDKNGVVHGKGSEAHKAAVTGDTVGDPYKDTSGPAVNPMIK
                     ITNIVALLLLAVLASGKIG"
     gene            complement(439..918)
                     /gene="bamE"
                     /locus_tag="VF633_05720"
     CDS             complement(439..918)
                     /gene="bamE"
                     /locus_tag="VF633_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX8470591.1"
                     /translation="MPRIASILAVAALAVSAAACAPVVGLHGFQVVDVKPSDIVAGTD
                     TKETVLARLGTPSTTSTFEPGQVWYYISQTSERYTYNRPQISQRSITEITFNKDDSKV
                     AGVRTLGLADGEKIAMEGRETPTRGRSLTIMEQLLGNVARGLLPRTDDDVPGLRRPD"
     gene            1007..1549
                     /locus_tag="VF633_05725"
     CDS             1007..1549
                     /locus_tag="VF633_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX8470592.1"
                     /translation="MLKSLFRKRPSQSQGDPLYAAVAAQARQPVFYTSLGVPDRIDAR
                     FELYTLHALLLILRLRDEDKVANGGEGAEAAQSLFDSYVSALDNVLRELGVGDVSMAK
                     KMRTLGEALYGRMTAYETPLRAGDAATLSTGLARNVFESEDAERGAALARYAIAAREG
                     LAAQTFADILKHPSWPEIAA"
     gene            1546..2100
                     /locus_tag="VF633_05730"
     CDS             1546..2100
                     /locus_tag="VF633_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX8470593.1"
                     /translation="MTEVLPYSESVRLNEIGAGLKRTLTPDAAMRARIARALDLASLD
                     AFEAELLLEPNPDHGASASKGGGGWRLSGRVKASAVQTCGISLEPLPVEIDERFSVDL
                     VEQAPAVAEDEEIEVGVDDDFPDVIEDGRIDLGQYAVEQLVLTLDPFPRKPGVEFVQP
                     DEPAEISPFAVLKAFKPKDDGSES"
     gene            2157..3293
                     /gene="plsX"
                     /locus_tag="VF633_05735"
     CDS             2157..3293
                     /gene="plsX"
                     /locus_tag="VF633_05735"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX8470594.1"
                     /translation="MSVASDDARRAYLLPSPVLISLDAMGGDHGPSVVVPAVREYIRH
                     HGGENVRFLLHGDEAAIAAEMARCKLASDVCQIRHTDKVVSMDEKPAQAMRRGKGSSL
                     WNAVESVKSGEAGAVVSAGNTGALMAISKLILRMSAPGLERPAIVASWPTLKGVTAVL
                     DVGANIESDAEQLIEFAIMGEAFHAAVHGKKTPTVGILNVGSEDMKGHDEVREAHQIL
                     KAGGLDLNYHGFVEGDDIAKGTVDVVVTDGFTGNIALKTAEGTARFISALLKDALTSS
                     VQAKMGALIAMPALKAMSRKIDPSTINGGPLLGLNGIVVKSHGGATAVGFANAIRIAV
                     DLARSDYLATVGAKLSRLGDILHAIPTPPEAGGAVVASSGETLQ"
     gene            3290..>4001
                     /locus_tag="VF633_05740"
     CDS             3290..>4001
                     /locus_tag="VF633_05740"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX8470595.1"
                     /translation="MSVIRSAVTGVGSYLPEQIVTNADLAKFVDTSDEWIQERTGIKQ
                     RHRARDDEPTSDLATEAARRALAAAGKTPADVDLIIVATTTPDMTFPATATIVQRKLG
                     CTVNIAFDVQAVCSGFVYALSIADGFIARGHAKCALVIGAEEMTKLMDWTDRTTCVLF
                     GDGAGAFVVEPREGQGTKEDQGILGFALRADGSKTDMLYVDGGPATTGTVGKLRMLGN
                     QVFRHAVVNIAEAITACCA"
BASE COUNT          721 a         1290 c         1311 g          679 t
ORIGIN      
        1 ccggtgctct cgccgatcgt gctgttcctg gccgtgctgc tgatctcgga caaggccaac
       61 gccttcgcgg cgctgggggc catgctgatg ggggtgatcg tgaccgggct gttcgtggcc
      121 atctcgatga cctcgggcgg cggggcgtgg gacaacgcca agaaggtgat cgaggaaggt
      181 ttcaccgaca agaacggcgt cgtccacggc aagggctcag aggctcacaa ggccgcggtg
      241 accggcgaca cggtcgggga tccctacaag gacacctccg gcccggcggt gaacccgatg
      301 atcaagatca ccaacatcgt ggcgctgttg ctgctggcgg tgctggcgag cggcaagatc
      361 gggtaggtct tagcctctgg aaaagaaaag ggcccggaga tttctctccg ggcccttttt
      421 gcttgtctgc agcggagctt agtccgggcg gcgcaggcct gggacgtcat cgtcggtgcg
      481 cggcagcagg ccacgggcga cgttgccgag cagctgttcc atgatcgtca gcgaccgacc
      541 acgggtcggg gtctcgcgac cttccatggc gatcttctca ccatcggcca ggcccagggt
      601 gcggactccg gcgaccttgc tgtcgtcctt attgaaggtg atctcggtga tgctgcgctg
      661 ggaaatctga gggcggttgt aggtgtaccg ctcgctggtc tggctgatgt agtaccagac
      721 ctgacccggc tcgaacgtcg aggtcgtcga tggcgtgccc agacgggcga gcacggtttc
      781 ctttgtgtcg gtgccggcga cgatgtcgga cggcttgaca tcaaccactt gaaagccgtg
      841 cagacccacg acgggggcgc aggctgcggc cgaaaccgcc agtgcggcga cggcgagaat
      901 agaggcgata cggggcatgg acgggagcct gcaacagaac aaggtctttg acctaaccgg
      961 acacgggctc tagttcaacg gcgcggcgga aaaggggccg tgaaacatgc tgaagtcact
     1021 ctttcgaaaa cgcccgtccc agtcccaggg cgacccgctg tatgcggccg tcgcggcaca
     1081 ggcgcgccag ccggtcttct atacgagcct cggcgtccct gaccggatcg atgcgcggtt
     1141 cgaactctac accttgcacg ccctgctgct gattctacgc cttcgcgacg aggacaaggt
     1201 cgcaaacggg ggggaggggg cggaggccgc ccagtcgctg ttcgacagct atgtctccgc
     1261 gctcgacaat gtgctgcgcg aactcggcgt cggcgacgtg tcgatggcca agaagatgcg
     1321 caccctcggc gaggcgctct atggtcggat gaccgcttat gagaccccgc tccgcgcagg
     1381 cgatgcagcc accctgtcca ctggactggc gcgcaatgtg ttcgagagcg aggacgcgga
     1441 acgcggcgcg gcgcttgccc gctatgccat tgcagcgcgt gaaggtctgg ccgcccagac
     1501 gttcgccgac attctgaaac accctagctg gccggagatc gccgcatgac cgaggtcctt
     1561 ccgtacagcg agagcgtccg tctcaacgag attggcgcag gcctgaagcg cacgctcacg
     1621 cctgacgcgg ccatgcgcgc gcggatcgcc cgcgcgctcg atctcgcctc gcttgatgcg
     1681 ttcgaggctg agttgttgct ggagccgaac cccgatcacg gagccagcgc cagcaaaggc
     1741 ggcggcggat ggcgtctgtc gggacgggtc aaggccagcg cggtccagac ctgtggcatc
     1801 tcgctggagc ccctgccggt cgagatcgac gagcgattct cggtcgatct tgtcgaacag
     1861 gctcctgcgg tcgccgagga cgaggaaatc gaagtcggcg tcgatgacga cttccccgac
     1921 gtgatcgagg acggtcgaat cgacctcggc cagtacgccg tcgagcaact ggtcctgacg
     1981 ctcgacccgt ttccccggaa acccggcgtc gaattcgtgc agcctgatga gcccgccgaa
     2041 atctcgccct tcgcggtgct taaggcgttc aagccgaagg acgacgggtc ggaatcctga
     2101 ccttgggtca cctgcctttg catcaccctg ttgcaggtct atcctgaacg gcccagatga
     2161 gcgtcgcgtc tgacgacgcc cggagagcgt accttttgcc atcccccgta ctgatttccc
     2221 tcgacgccat gggtggcgac cacggaccct ccgtcgtcgt cccggctgtg cgggaataca
     2281 tccgtcatca tggcggcgag aacgtgcgct tcctgctgca cggcgacgag gccgcgatcg
     2341 ccgccgagat ggccaggtgc aaactggcta gcgacgtctg ccagatacgc cacaccgaca
     2401 aggtcgtctc catggacgaa aagccggccc aggccatgcg ccggggcaag ggatccagcc
     2461 tgtggaatgc ggtcgaatcg gtgaagtccg gcgaggccgg agccgtcgtc tccgcaggta
     2521 acaccggggc cttgatggcc atttccaagc tgatcctgcg catgtcagcg ccggggctag
     2581 agcgcccagc aatcgtcgcc agctggccta cgctgaaagg cgtcaccgcc gtgctcgacg
     2641 tcggcgcgaa catcgagagc gacgccgagc aactgatcga attcgccatc atgggcgagg
     2701 ccttccacgc cgccgtccac ggcaagaaga cgccgacggt cggcattctc aacgtcggtt
     2761 ccgaggacat gaagggtcat gacgaggtgc gcgaggcgca tcagatcctc aaggccggcg
     2821 ggctcgacct gaactatcac ggcttcgtcg agggcgatga catcgccaag ggcacggtcg
     2881 acgtcgtcgt gaccgacggc ttcactggca atatcgcgct gaagaccgcc gagggcactg
     2941 cgcgtttcat ctcggccctg ctgaaggacg cgctgacctc cagcgttcag gccaagatgg
     3001 gcgcgctgat cgccatgcct gcgctcaagg cgatgagccg caagatcgat cccagcacca
     3061 tcaacggcgg gccgttgctg ggcctgaacg gcatcgtcgt gaagagccac ggcggcgcaa
     3121 cagcagtagg cttcgccaac gccatccgca tcgccgtcga tctggcccgc agcgactatc
     3181 tggccaccgt gggcgccaag ctgagccgtc tcggcgacat cttgcacgcc attccaaccc
     3241 cacctgaagc gggcggcgct gttgtcgcct cctctggaga gaccctgcag tgagcgtcat
     3301 ccgtagcgcc gtcaccggcg tcggttccta tctgcccgag cagatcgtca ccaacgccga
     3361 cctggcgaag ttcgtcgaca ccagtgacga atggattcag gaacgcaccg gcatcaagca
     3421 gcgccatagg gcgcgcgacg acgaaccgac cagcgatctg gccacagaag cggcccgccg
     3481 tgctctggcg gctgccggca agacccccgc cgacgttgac ctgatcatcg tcgccacgac
     3541 cacgccggac atgacgttcc cggcgacggc gaccatcgtc cagcgcaagc tcggctgtac
     3601 ggtcaacatc gctttcgacg ttcaggccgt ctgctcgggc ttcgtctatg cactcagcat
     3661 cgccgacggt ttcatcgcgc gaggccatgc gaaatgcgcc ttggtcatcg gcgccgagga
     3721 gatgaccaag ctgatggact ggactgaccg gaccacctgc gtcctgttcg gtgacggcgc
     3781 cggcgccttc gtcgtcgagc cgcgcgaagg gcaggggacc aaggaagatc agggcatcct
     3841 cggcttcgcc ctacgggccg acggttccaa gaccgacatg ctgtacgtcg atggtggccc
     3901 ggcgacgacg ggcacggtcg gcaagctgcg gatgcttggc aatcaggttt tccgccacgc
     3961 ggtcgtcaac atcgccgagg ctatcaccgc ctgctgcgct g
//
LOCUS       DASVTM010000213         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_234194,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000213 DASVTM010000000
VERSION     DASVTM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234194"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VF633_05745"
     CDS             complement(<1..947)
                     /locus_tag="VF633_05745"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8470596.1"
                     /translation="MTQGPLSGVRVLDLSRVLAGPWATQLLADLGAEVIKIERPGAGD
                     DTRAWGPPFLKTTDGTRGDAAYFLSANRGKKSVTLDIATDEGAAIARELAATCDVVIE
                     NFKTGGLKKYGLDHASLTAAHPKLVYCSITGFGQDGPDAGRPGYDYMIQAMGGLMSIT
                     GQPDGAPGAEPMKVGVAVVDLFTGMYASNAILAALLHARATGEGQHIDLALFDVQAAM
                     LANQATNYFASGSAPTRMGNAHPNLAPYQPFPCSDGMIVVAVGNDGQFRAFAGALGAA
                     EMGTDPRFATNALRIEHRAMLTATVSALTAGLTMRELTVA"
     gene            complement(988..1254)
                     /locus_tag="VF633_05750"
     CDS             complement(988..1254)
                     /locus_tag="VF633_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HEX8470597.1"
                     /translation="MIRRLKTLFADHPREVNETYLEHQATAFGMGLKLARLSATAFLH
                     GIVPGVCKSSVSDEIKAMARDLGGRAEEAKECRMRDAGVWDPGL"
     gene            1382..2572
                     /locus_tag="VF633_05755"
     CDS             1382..2572
                     /locus_tag="VF633_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8470598.1"
                     /translation="MADSAAPSVQTFKWDDPFDLTARLTEDERAIQEAARSYARDKLL
                     PRVVAAFREENFDRAIMTELGEMGFLGAMLPEQYGGSGVSHVAYGLISREIEAVDSGY
                     RSAMSVQSSLAMYPIYAFGSEDQKMRFLPRMAAGELIGCFGLTEADGGSDPASMKTKA
                     EAVEGGYRLNGGKYWITNSPISDLAVVWAKLDGVIRGFIVEREFEGFTTTEIGDKLSL
                     RASITGDIGLADVFVPEANLLPGVKGLRGPFSCLNKARYGIAWGAMGAAEFCFHATRD
                     YVGERMLFGRPLSSRQLVQKKLADMQTEIFLGFEGAYALGRLLDTGAWVPEAISMMKR
                     NNCGKALAIAREARDMHGGAGITGEMHVMRHAMNLETVNTYEGAHDVHALILGRAITG
                     ESAF"
     gene            2725..>3349
                     /locus_tag="VF633_05760"
     CDS             2725..>3349
                     /locus_tag="VF633_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8470599.1"
                     /translation="MSRAIFMAGSAVALVLATLPGVTLAQTSSPTPAPATSAPAASQT
                     PYRAPRPATPPAPVVRSPQYPLPYDEKRGVFTFATGLERSLPAVVQVTTLGTSRGPTT
                     GETEPKPLQSGSGVIIDAREGIIVTNNHVVDGGTKFTVDMTDGRLFDATLVGVDKATD
                     VAVLRIDTPDIRSLGLSQVETVNSDTLRTGDLAFAVGYPLGLDQTLTM"
BASE COUNT          567 a         1146 c         1059 g          577 t
ORIGIN      
        1 gccacggtca gctcccgcat cgtcagtccg gcggtcaagg cgctaacggt ggcggtcagc
       61 attgcgcgat gctcgatgcg caaggcgttg gtggcgaagc gcggatcggt ccccatctcg
      121 gcggcgccca gcgctccggc aaacgcccgg aactgaccgt cgtttccgac cgcgacgacg
      181 atcatcccgt ccgagcatgg gaatggctga tacggcgcga ggttcgggtg ggcgttgccc
      241 attcgcgtcg gcgcggaccc cgaggcgaaa tagttggtcg cctgattggc cagcattgcg
      301 gcctgaacgt cgaacagggc aaggtcgata tgctgcccct cccccgtcgc ccgcgcatgc
      361 aaaagagcgg cgaggatggc gttcgacgcg tacatgccgg tgaacagatc gaccaccgcg
      421 accccgacct tcatcggctc ggcgccgggc gcgccatccg gctggccagt gatcgacatc
      481 agcccgccca tggcctggat catgtagtcg tagcccggcc gtccggcgtc cggcccgtcc
      541 tgaccgaagc cggtgatcga gcaatagacg agctttggat gcgccgccgt caggctggcg
      601 tggtcgaggc cgtatttctt caggccaccg gtcttgaagt tctcgatcac gacgtcgcag
      661 gtcgcggcca gctcacgcgc gatcgcggcc ccctcgtccg tagcgatatc cagcgtgacc
      721 gatttcttgc cccggttggc cgacaggaaa taggccgcgt ctccgcgtgt gccatccgtc
      781 gtcttcagga agggcggtcc ccacgcgcgg gtgtcgtccc ccgcgccggg tcgctcgatc
      841 ttgatcacct cggcgccaag gtccgccagc aactgtgtcg cccacggtcc ggcgaggaca
      901 cgtgacaggt cgagcacgcg aacaccggag agagggcctt gggtcatcct gtgtgactac
      961 cccttctccc cctgcgggag aagtgagcta aagacccgga tcccacacgc ccgcgtcgcg
     1021 catgcggcat tccttggctt cctcagcgcg accgccgagg tcgcgcgcca tcgctttgat
     1081 ctcgtccgag acagaggact tgcacacgcc gggcacgatc ccgtgcagga acgcggtcgc
     1141 cgataaacgg gccagcttca gccccatacc gaaggcggtc gcctgatgtt cgagatacgt
     1201 ctcgttcacc tcgcgagggt gatcggcgaa cagcgtcttc agcctgcgga tcatgaattg
     1261 ccctctgaca cgacggacct gccactaccc gacgcagcca ttctgcccta aaccgcaagc
     1321 aacaacaggg gccggctcct cacgatccgc tgaacgccct gagagacacc gggaagacga
     1381 catggccgac agcgccgcac cctccgttca aaccttcaaa tgggacgacc cgttcgacct
     1441 gaccgcccgc ctgaccgagg acgagcgcgc cattcaggag gccgcgcgct cctatgcccg
     1501 cgacaagctg ctgccgcgcg tggtcgccgc cttccgtgag gagaatttcg accgcgccat
     1561 catgaccgag ctcggcgaga tggggttcct cggcgccatg ctcccagaac agtacggcgg
     1621 atcgggcgtc agccacgtcg cctatggcct gatctcgcgc gagatcgagg cagtcgacag
     1681 cggttatcgt tcggccatga gcgtccagtc gtcgctggcc atgtacccga tctacgcctt
     1741 cggttcggag gatcaaaaga tgcgcttcct tccccggatg gcggctggcg agctgatcgg
     1801 ctgcttcggc ctgaccgagg ccgacggcgg ttcggatccc gcgtcgatga agaccaaggc
     1861 ggaagccgtc gagggcggct atcgcctgaa cggcggcaaa tactggatca ccaactcgcc
     1921 gatttcagac ctcgcggtcg tgtgggccaa gctggatggc gtcattcgtg gctttatcgt
     1981 cgagcgtgag ttcgagggat tcaccaccac cgaaatcgga gacaaactgt ccctgcgcgc
     2041 ctccatcacc ggcgacatcg ggctggccga tgtgttcgtg cccgaggcca atctgctgcc
     2101 gggagtgaag ggtctgcgcg gaccgttctc ctgcctgaac aaggcgcgct acggcatcgc
     2161 ctggggggcc atgggagcgg ccgagttctg cttccacgcc acacgcgact acgtcggcga
     2221 acgtatgctg ttcggtcgtc cgctgtcgtc gcgccagctc gtgcagaaga agctggccga
     2281 tatgcagacg gagatctttc tcggcttcga aggcgcctat gcgctgggcc gtttgctgga
     2341 caccggcgcc tgggtgcctg aggcgatttc gatgatgaag cgcaacaact gcggcaaggc
     2401 tctggccatc gcccgcgagg cccgcgacat gcacggcggc gccggcatca ccggtgaaat
     2461 gcacgtcatg cgccacgcca tgaacctcga gaccgtcaac acctacgagg gggcccacga
     2521 cgtccacgcc ctgatcctcg ggcgggcgat caccggcgaa agcgcgtttt agagggggcg
     2581 acttcgtcgc cgcgctgacg ctcacgacgc ggtcgttcgc tgcttgagcg cgccgtgtct
     2641 tggaccatcg tcacggcgca tttctcacgc taggctgtta cccggtcgtg agcgaagacg
     2701 cgtcgcgccc gggggaaaga cttgatgtca cgtgcgattt tcatggcggg ttcggccgtt
     2761 gctctggttc tagctaccct cccgggtgtt accctggcgc agacgtcgag cccgacgccg
     2821 gcgccagcca ctagcgcccc ggccgcgtcc cagacgccct accgcgcgcc tcgtccggcc
     2881 acgcctccgg ctccggtcgt gcggtcccct cagtatcccc tgccctatga cgagaagcgc
     2941 ggcgtcttca ccttcgccac cggcctcgag cgctcgctgc ccgcggtggt gcaggtgacg
     3001 acgctcggca cttcccgcgg gccgaccacc ggcgagacgg agcccaagcc tctgcagagc
     3061 ggatccggcg tcattatcga tgcgcgcgaa ggcatcatcg tcaccaacaa tcatgtggtc
     3121 gacggcggca cgaaattcac cgtcgacatg acggacggtc ggctattcga cgcaacgctc
     3181 gtcggcgtcg acaaggctac cgacgtcgcg gtgttaagga tcgacacgcc cgacatccgc
     3241 tcgctgggcc tgtcgcaggt cgagaccgtc aattccgaca ccctgcgcac cggcgacctc
     3301 gcctttgcgg tcggctatcc gctgggcctc gaccaaacgc tgaccatgg
//
LOCUS       DASVTM010000214         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_925463,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000214 DASVTM010000000
VERSION     DASVTM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925463"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1116
                     /gene="fliG"
                     /locus_tag="VF633_05765"
     CDS             79..1116
                     /gene="fliG"
                     /locus_tag="VF633_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX8470600.1"
                     /translation="MARPAGKKPSVDDVKKLTGPEKAAVILLSLGEEHTQLWKGLDDE
                     EIKEISQAMAGLGTVSAEVVEELLLEFVSGMSGSGSVMGSFEQTQRLLAAFMPSDRVD
                     GLMEEIRGPAGRTMWDKLGNVNEAVLANYLKNEYPQTVAVVLSKVKPDHAARVLTALP
                     EDFALECVQRMLRMEPVQREILDKIEQTLRVEFMSNLARTSKRDSHEMMADIFNSFDR
                     QTEARFIGALEERNREAAERIRALMFVFEDLNKLDPGGVQTLLRAVEKDQLALALKGA
                     SETLREMFFTNMSERASKIMREDMESMGPVRLKDVDSAQMAMVQAAKDLAAKGEIMLA
                     GAGGADDELIY"
     gene            1118..1777
                     /locus_tag="VF633_05770"
     CDS             1118..1777
                     /locus_tag="VF633_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FlbE"
                     /protein_id="HEX8470601.1"
                     /translation="MTIAQPLNSRPFSFDTEFDDVGGVTAGSVFRPAKRAYLPAEVDA
                     LVAQARLEARETALAEVESLQAMALVTLGQAIAAAAPSLASVAQVHREQSAELAMAAA
                     RVIATAALDRFPEGPLQAALEALGSEIDATPRLVIRAHGLDDAVKARLQALCVDAGFS
                     GLVSFRDGPGMAAAAFQLEWADGRADFDPAEAAARMGEALTAALAAEAGHAETINGSN
                     F"
     gene            1777..2106
                     /gene="fliN"
                     /locus_tag="VF633_05775"
     CDS             1777..2106
                     /gene="fliN"
                     /locus_tag="VF633_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX8470602.1"
                     /translation="MAEDFALEEFGASTALAIGDEGGDKSAADLAPVFDVPVNISAVL
                     GKAHMTVAQLLKLNRGSVLELDRKVGEAIDIFVNNRLVARGEVVVVEDRLGVTMTEII
                     KTEDSAA"
     gene            2146..>2514
                     /locus_tag="VF633_05780"
     CDS             2146..>2514
                     /locus_tag="VF633_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX8470603.1"
                     /translation="MRLLVVGRLSGQLASAVKMAMAHGAKVQHVERTDQATEQLRRGQ
                     GADLLMVDYNLDIAGLIAANDAERIFVPVVACGVDNDAQKAAAAIRAGAKEFIPLPPE
                     ADLIAAVLSAVADDERPLISA"
BASE COUNT          451 a          802 c          831 g          430 t
ORIGIN      
        1 caagaaggtg gcggacttcg tgcagaagca cccggatgaa tcgaccgcga ttctccgcag
       61 ttgggttcag gaaggctgat ggctcgtcca gccggcaaga agccttccgt cgacgacgtc
      121 aagaagctga cagggccaga aaaggccgcc gtgatcctgc tttcgctggg cgaggagcat
      181 acccagctgt ggaaggggct cgacgacgaa gagatcaagg agatctctca agcaatggcc
      241 ggacttggta cggtcagcgc cgaggtcgtc gaggagctct tgctggagtt cgtctcgggc
      301 atgagcggct cgggctcggt catggggtcg ttcgaacaga cccagcggct gctggccgcc
      361 ttcatgccct ccgatcgcgt agacggcctg atggaggaga tccgcggtcc cgccggtcgg
      421 accatgtggg acaagctcgg caacgtgaac gaggctgtgc tcgccaacta tctcaagaac
      481 gaatacccgc agaccgttgc cgtggttctg tccaaggtga agcctgatca cgccgcgcgc
      541 gtcctgacgg cgctgcccga ggacttcgcc ctcgaatgcg ttcaacggat gctgcgcatg
      601 gagccggtgc aacgcgagat cctcgacaag atcgaacaaa ccctgcgcgt cgagttcatg
      661 tccaacctgg cccgcacgtc gaagcgcgac agccacgaga tgatggccga catcttcaac
      721 tcgttcgatc gccagaccga ggcccgcttc atcggcgccc tcgaagagcg caaccgcgag
      781 gcggccgagc gtatccgcgc cctgatgttc gtcttcgagg acctgaacaa gctcgatccg
      841 ggcggcgtcc agacgcttct gcgcgcagtt gagaaggacc agctcgccct ggccctcaag
      901 ggggcgtccg agaccctgcg cgaaatgttc ttcacgaaca tgtccgagcg cgcttccaag
      961 atcatgcgcg aagatatgga atcgatggga cccgtccgtc tcaaggacgt cgacagcgcc
     1021 cagatggcca tggtccaggc cgccaaggac ctcgccgcca agggcgagat catgctggcc
     1081 ggggctggcg gcgccgatga cgagttgatc tactgacatg accatcgccc agcctctcaa
     1141 cagtcgtccc ttcagcttcg acaccgagtt cgatgacgtc ggcggtgtga ccgccggttc
     1201 ggtcttccgt ccggcgaagc gcgcctattt gcctgccgag gtagatgcgt tggtggccca
     1261 ggcccgtctg gaagcgcgcg agacggccct tgccgaggtc gagagcttgc aggcgatggc
     1321 gttggtcacg ctcggccagg ccatcgctgc cgcagcgccc tctctggctt cagttgcgca
     1381 ggtccaccgt gagcaatcgg ccgaactggc catggccgcc gcccgcgtca tcgcaacggc
     1441 ggcgctcgac cgcttcccgg aagggccgct tcaagctgcg ctggaggctt tgggctccga
     1501 gatcgacgct acgccgcgtc tggtcattcg ggcgcatggc ctcgatgacg cagtgaaggc
     1561 ccggctgcag gccttgtgcg tcgatgccgg gttctcgggt ctggtgtcat tccgcgatgg
     1621 accgggcatg gccgccgccg ccttccagct cgaatgggcc gacggccgcg ccgatttcga
     1681 tcctgccgag gctgcagccc gcatgggcga ggccctgacc gccgccctcg ccgccgaggc
     1741 cggccatgct gaaacaatca acgggagcaa cttctaatgg ccgaggattt tgccctggag
     1801 gagtttgggg cctccaccgc actggcgatc ggcgacgaag gcggggacaa gagcgccgcc
     1861 gatctcgcgc ccgtgttcga cgtgccggtc aatatctcgg ccgtactggg caaggcgcac
     1921 atgacggtgg ctcagctgct caagctgaac cgcggcagcg tgctggaact ggaccgcaag
     1981 gtcggcgagg ccatcgacat cttcgtcaac aaccggctgg tcgctcgcgg ggaggtcgtc
     2041 gtggtcgagg atcgcctggg cgtgaccatg acggaaatca tcaagaccga ggactcggcg
     2101 gcctaggtcg ctggatcgcg cgtaagagct tagaggagaa gaccaatgcg gcttctggtg
     2161 gttggacggc tttcgggcca gcttgcgtca gcagtgaaga tggccatggc gcacggcgcc
     2221 aaggtccagc acgtcgagcg tactgatcag gcgacggaac agctccgtcg cggacagggc
     2281 gccgacctgc tgatggtcga ctacaacctc gacatcgccg gtctgatcgc cgccaatgac
     2341 gccgagcgga ttttcgtgcc tgtcgtggcc tgcggcgtcg acaacgacgc gcaaaaggcc
     2401 gccgccgcca tccgcgcggg ggccaaggag ttcatcccgc ttccacctga agccgatctg
     2461 atcgccgctg ttctttcggc ggtcgccgac gacgagcgtc cgctgatctc ggcc
//
LOCUS       DASVTM010000215         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_125676,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000215 DASVTM010000000
VERSION     DASVTM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125676"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..564)
                     /locus_tag="VF633_05785"
     CDS             complement(91..564)
                     /locus_tag="VF633_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtaL-like protein"
                     /protein_id="HEX8470604.1"
                     /translation="MIYSTAAVPVNPAGQTVLTRAQIWASLELKARDARLFLPEGLCT
                     RCDVVEESATHFIRGATIAGAELREIITLEPERKVTFFQATGPREGAIVNELFEDEDG
                     ALQLRFYCYLGLRDRAPDGPEEKAEQARFDRDEGYRAALLSTLGRTRELVAQGKL"
     gene            complement(590..1462)
                     /locus_tag="VF633_05790"
     CDS             complement(590..1462)
                     /locus_tag="VF633_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEX8470605.1"
                     /translation="MTILVTGATGRVGRHVVQSLIDRGADVRVLVRDIAMADFPARVE
                     VAQGDMLDLDALRAAFSGVRTLFLLNAVAADEFTQALITLNVAREAGVERVVYLSVIH
                     SERFVNVPHFAVKSGAERMIEQMGFSATILRPAYFIDNELMIKDVILGYGVYPMPIGD
                     KGVAMVDARDIAEAAALELIRRDAAETRLPITTLNLVGPDTLTGERIAAIWSEVLGRP
                     IAYGGDDPTAFEQNLATLMPKWMAYEMRLMAERYVSDGMIPEPGDVERLTALLGRPLH
                     TYRDFAARIAADAA"
BASE COUNT          276 a          501 c          570 g          282 t
ORIGIN      
        1 gcttgtcaca ctgtcgcccg accgccgcct tcggcgcggt cggggcccgg gtcaacggag
       61 cctcgacccc cgaggctccg ttgacgaaca tcagagctta ccttgcgcga ccagttcgcg
      121 ggtccggccg agcgtcgata gcagggcggc ccgatagcct tcgtcacggt cgaaccgggc
      181 ctgctccgcc ttctcttcgg ggccgtctgg tgcacgatcg cgcaggccca gatagcaata
      241 gaaacgaagc tgcagtgcgc cgtcctcatc ttcgaacaac tcgttgacga tggcgccttc
      301 gcgaggtcct gtggcctgga agaacgtcac cttccgctcc ggctcaaggg tgatgatctc
      361 gcgcagctcg gcgcctgcga tggtggcccc ccgtatgaaa tgggtcgcgc tctcctcgac
      421 cacgtcgcag cgggtgcaca gaccttccgg caggaagaga cgtgcgtcgc gcgccttgag
      481 ctccaggctg gcccagatct gtgcccgggt gaggacggtc tggccggcgg ggttcaccgg
      541 cacggcggcg gttgaataga tcatgtcgtg tctccttggc gttgagggat caggccgcgt
      601 ctgcggcgat gcgggcggcg aagtcgcgat aggtgtggag cggacggccg agcagggcag
      661 tcagacgctc gacgtcgccg ggctcgggga tcatgccgtc gctgacatag cgctcggcca
      721 tgaggcgcat ctcgtaggcc atccacttcg gcatcagagt ggccaggttc tgctcgaagg
      781 ccgtggggtc gtccccgcca tatgcgattg gccgcccgag gacttcggac cagatggcgg
      841 cgatgcgctc acccgtcagg gtgtcaggcc cgacaaggtt gagggtggtg atcggcaggc
      901 gcgtctcggc cgcgtcacgg cggatcagtt cgagggcggc ggcctcggcg atgtcgcggg
      961 cgtcaaccat ggccacgccc ttgtcgccga tcggcatcgg gtagacgccg tagccgagga
     1021 tcacgtcctt gatcatcagt tcgttgtcga tgaaataggc cggtcgcagg atggtggcgc
     1081 tgaagcccat ctgctcgatc atccgctccg caccggactt cacggcgaag tgcggaacgt
     1141 tgacgaagcg ctcgctgtgg atgaccgaca gatagacgac ccgctcgact ccggcttcgc
     1201 gagcgacatt cagggtgatg agcgcctggg tgaattcgtc ggcggccacg gcgttgagca
     1261 ggaacagcgt gcggacgccg gagaacgcgg cgcgcagggc gtccagatcg agcatgtcgc
     1321 cctgggcgac ctccacccgg gcagggaagt cggccatggc gatgtcgcgg acaaggacgc
     1381 ggacatcggc gccgcgatcg atcaggctct gcacgacatg gcggccgaca cggccggtgg
     1441 cgccggtaac gaggatggtc atgagaagtc tccgggttgg ggtgagggaa cacccggaaa
     1501 atcaacgatc cacttccgat ccgatagacg ctagtttgag accaactgtc tcataggcgg
     1561 aacacatgga cctgttagcg ctcgcggatt tcaatctcgt ggcccggcac gaggggtttg
     1621 ggcgcgctg
//
LOCUS       DASVTM010000216         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_338327,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000216 DASVTM010000000
VERSION     DASVTM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338327"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VF633_05795"
     CDS             <1..506
                     /locus_tag="VF633_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX8470606.1"
                     /translation="GASSRTMLRNDAYWFLRLGGAIERADNTARLLDVKYHLLLPADE
                     RVGGQLDYFQWTTLLREVSALTAYRWVYRESVRPWLVADLMVLNRQMPRSLASCQGMI
                     VSYLERLATDYGRRGPSQRLASARLDQFNNAKIEDIFQSGLHEYILCFLNENNNLAAA
                     VHDQYLV"
     gene            511..1353
                     /locus_tag="VF633_05800"
     CDS             511..1353
                     /locus_tag="VF633_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX8470607.1"
                     /translation="MRIRIDYETKYSYARPARFIVQTLRVTPGSTESQQVRDWRIETD
                     VDTRLRRSEDAFGNIVHTLYTERPTGVLTIRVTGEVATNDTGGVLRSGPERLSPLVYL
                     RDSSLTHADAALREFADQVGEEGDTLARLHRLMAAIHSEVAFMVGSTTADNTAADAFA
                     QRRGVCQDHTQIFIACARRMGIPARYVSGHLHRTDGLIDQDAAHAWAEAWVQDLGWVG
                     FDAANGIAPTEHYVRVAAGLDALGASPFRGTSYGGGPETMTVALRVRQMQQTQQQQQT
                     LSWS"
     gene            1438..2211
                     /locus_tag="VF633_05805"
     CDS             1438..2211
                     /locus_tag="VF633_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX8470608.1"
                     /translation="MTYCVGMLVNDGLAMIADTRTNAGVDNISSYKKLHITEVPGERI
                     ISICTAGNLSVTQTALAMVGEGIKMPGHDDFETLETTPSLFRAAQLVGYALNQVRKEI
                     APSLEADSLKVSASMLLGGQVRGGKMGLYLIYAQGNFIECSADTPYMQIGELKYGKPI
                     LDRALRPDTPMAEAVKLGLISFDSTIRSNIAVGPPFDMVVIKRDTLHGEQRRIDADDP
                     YFKDLGRRWSEALANAHRNMPPPPWLEPAAVRPAISVVG"
     gene            2275..>2723
                     /locus_tag="VF633_05810"
     CDS             2275..>2723
                     /locus_tag="VF633_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX8470609.1"
                     /translation="MTEETILAEGAPDGDAGALKAADLIRDEARRAPDKPGVYRMYGE
                     DGTCLYVGKAKSIKKRILQYAQGRFHTQRIGLMVSLTRSMQLVVTASETEALLLESNF
                     IKKLKPRFNVVLRDDKSFAELMIRRDHRAPQVRKHRGAHNVPGDYFGP"
BASE COUNT          510 a          880 c          836 g          497 t
ORIGIN      
        1 agggcgcgtc gtcccggacc atgctgagga atgacgccta ctggttcctt cgtctgggcg
       61 gagcgatcga gcgggctgac aacaccgccc gcctgctgga cgtgaaatat cacctgttgc
      121 ttcccgccga cgagcgggtc ggggggcagc tcgactattt ccaatggacc accctgctgc
      181 gcgaggtctc ggccctgacc gcctaccgct gggtttaccg ggagtctgtc cgcccctggc
      241 tggtcgccga tctgatggtt ctgaaccgcc agatgccgcg ttcactcgcc agctgtcagg
      301 gcatgatcgt cagctatctg gagcgcttgg ccacggacta tggtcgtcgc ggtccatcgc
      361 agcgtctggc gtccgcacgg ctcgaccagt tcaacaatgc gaagatcgaa gacatcttcc
      421 agtcaggtct gcacgaatac atcctctgtt ttctgaatga aaacaacaat ctggccgctg
      481 ccgtccacga tcagtatctg gtgtgacctg atgcgcatcc gtatcgatta cgagacgaaa
      541 tactcctatg cccgcccggc gcggttcatc gtccagacgc tgcgcgtgac gccgggctcg
      601 accgagagcc agcaggtgcg cgactggcgc atcgagaccg atgtcgacac ccggctgcgg
      661 cgttccgagg acgccttcgg caacatcgtg cacaccctct acacggagcg gccgaccggg
      721 gtgctgacca ttcgggtcac cggcgaggtc gcgaccaacg acaccggcgg ggtcctgcgt
      781 agcggtcccg aacggctttc accgctggtc tatttgcgcg acagctccct gacccatgcc
      841 gatgccgcgc tgcgcgaatt cgctgatcaa gtcggcgagg aaggcgacac cctggcccga
      901 ctgcaccggc tgatggcggc gatccattcg gaggtcgctt tcatggtcgg gtccaccacc
      961 gccgataata cggctgcgga cgcgttcgcg cagcggcggg gtgtctgtca ggatcacacc
     1021 cagatattca ttgcctgcgc acgccgcatg ggcatcccgg ctcgttacgt ctccggccac
     1081 ctgcaccgaa ccgatggact gatcgatcag gacgcggccc atgcctgggc ggaagcctgg
     1141 gtgcaggatc tgggctgggt cggcttcgat gcggccaacg gcattgctcc gacggagcac
     1201 tacgtccgcg tcgcagccgg tctggatgcc ctcggcgctt ctccgtttcg gggcaccagc
     1261 tacggaggcg gtccggaaac gatgaccgtg gccctccgtg tgcgccagat gcaacagacc
     1321 cagcaacaac aacagaccct gagctggtca tgagttggca agggtcgtcg tgcgacaaga
     1381 atctgttagc gttacccgac gctttgagca ttcgctcgtc gaggccgaac tgatctgatg
     1441 acctattgcg tcggcatgct ggtgaatgac ggtctggcga tgatcgccga cacgcgtacg
     1501 aacgcgggtg tcgacaacat ctcctcctac aagaagctgc acatcaccga ggtgccgggt
     1561 gagcgcatca tttcgatctg tactgcaggc aatctctcag tcacccagac cgccctggcc
     1621 atggtcggcg agggcataaa gatgcccggt cacgacgatt tcgagacgct ggaaacgacg
     1681 ccatccctgt tccgggcagc ccagttggtc ggatacgccc tgaaccaggt ccgcaaggaa
     1741 atcgcgcctt cgcttgaggc ggacagcctg aaagtctcgg catcgatgct gctggggggg
     1801 caggtccgtg gaggcaagat gggcctgtac ctgatctacg cccaaggcaa tttcatcgag
     1861 tgcagcgccg acacgcccta catgcaaatc ggcgagctca agtacggcaa gcccatcctc
     1921 gatcgcgccc tgcgtcccga cacgcccatg gccgaggcgg tcaagctggg tctcatctcc
     1981 ttcgactcga ccatccgatc caacatcgcc gtcggtccgc ccttcgacat ggtggtgatc
     2041 aagcgtgata ccttgcacgg cgagcagcgc cgcatcgacg ccgacgatcc ctatttcaag
     2101 gacctgggtc gccggtggtc agaggcgctg gcgaacgccc accgaaatat gccgcctccg
     2161 ccatggcttg aaccggccgc cgtacgtccg gcaatctcgg tggtcggata agcgcaccgt
     2221 tttctccctc agaggagaag ggcaaaccgt cggaaacctc ctatattgcc tccgatgacc
     2281 gaggagacga ttctggctga gggcgccccg gatggggatg ccggcgcgct gaaagccgcc
     2341 gacctgatcc gcgacgaggc gcgtcgcgca ccggacaagc cgggcgtcta tcgcatgtac
     2401 ggcgaagacg ggacctgcct ctatgtgggc aaggccaagt cgatcaagaa gcgcatcctg
     2461 cagtatgcgc agggccggtt ccacacccag cgcattgggt tgatggtgtc cctgacccgg
     2521 tcgatgcagt tggtggtcac tgcctcagag accgaggcgt tgctgctcga gtccaacttc
     2581 atcaagaagc tgaagccgcg tttcaacgtg gtgctcaggg acgacaagtc gttcgcggag
     2641 ctgatgatcc gccgcgacca ccgcgcgccc caggtccgga aacatcgcgg cgcccacaac
     2701 gtccccggcg actatttcgg acc
//
LOCUS       DASVTM010000217         5472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1030564,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000217 DASVTM010000000
VERSION     DASVTM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030564"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VF633_05815"
     CDS             <1..331
                     /locus_tag="VF633_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470610.1"
                     /translation="IRFPDDETMGAIVEVHFPGIRPRLVSAALKTFYEIRDTPGLKKK
                     PSTSELLDWLKLLLVDDIDPETLRETSPNKLIPPLHGALLKNEQDVHLFERLAFLTRR
                     EGGRPGS"
     gene            470..1411
                     /locus_tag="VF633_05820"
     CDS             470..1411
                     /locus_tag="VF633_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HEX8470611.1"
                     /translation="MLRLPTSYLPAPRPWSRLAMLAACASGLVLFAACDDSGVRISRS
                     TSNSDAEGVLKVVDALQCPESLGALTRKGSAQADGTVCTYTGPRGAQVELHLVTLDDD
                     GADPILKAFETRLLASMPHTAADISRSTADSARADANAARADAQAARADAEAAGADAR
                     AEADANPDSAHISAPGMKVDAEGDRASVHMPGIDVEADGDKADVRIGGFTIRANEGNA
                     SINGRLTTGDNTDSVNINARDNAAEIRTRAPGEAIRQTYLLTDNRPSDAGWRTVGYEA
                     RGPRTGPIVVATVRSRESNADGVFDDAKELVALNVGE"
     gene            1553..2146
                     /locus_tag="VF633_05825"
     CDS             1553..2146
                     /locus_tag="VF633_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEX8470612.1"
                     /translation="MLSGRRLDLLDPSPFDIEIEDIAHGLARVARWNGQTIGEHAFSV
                     AQHSCVVEEIAVHIKPDLAPKWRLAALLHDASEYVIGDMISPFKAALGMSYKDFEARL
                     EHAIHVRFGLPPRSPVAIKTLIKKADRACAFFEATQLAGFERAESLKIFGAPPPGYVL
                     IIDPLPAPIAQARYLERYRVLGEAVGIMPAADAWHTE"
     gene            2150..3088
                     /locus_tag="VF633_05830"
     CDS             2150..3088
                     /locus_tag="VF633_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HEX8470613.1"
                     /translation="MSFGGEHGVRIGLSAVVMALRDRRLCVLTTGTDEATALPFGPFD
                     PANDRTFELSLRAFITAQTGFRSGFVEQLYTFGDAGRESPRPTPGPERQREISVGYLA
                     LTRDASAADSADARWTPVFDAFPWEDRRDPTDAIRPLIAEALTRWAGSDPRRLARIDG
                     LFALGPGLRWNEERVLERYELLYEAGLVPEAARDGVCPVATALPGLTMASDHRRILAT
                     GLGRLRSKLKYRPVLFELAPETFTLSELQTAAEAVAGLPLHKQNFRRGVERTGLVEPT
                     GRLSAATGGRPAELFRFTGADPRGGQAPGLALPAQR"
     gene            3186..3275
                     /locus_tag="VF633_05835"
     tRNA            3186..3275
                     /locus_tag="VF633_05835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3220..3222,aa:Ser,seq:cga)
     gene            complement(3618..4577)
                     /locus_tag="VF633_05840"
     CDS             complement(3618..4577)
                     /locus_tag="VF633_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8470614.1"
                     /translation="MTKHLLLVGLTLACFAAPGISSAQATRTWVSGVGDDVNPCSRTA
                     PCKTFAGAISKTAAGGEINVLDPGGFGTVTITKSITITTDPGFGGILASSTTGVTINA
                     GATDVVTLRGLYINGSIGASTGLNGVRILAAGTVNIQNSAIMGARSPSAGYGVALLNS
                     AGTTYVNIADTTISESGTGSTGSAIQIAPTGSGSAFVSIRNSRILNNTRGVVIDSSAT
                     AGAVHTSISGSLISGNSLAGVLAISLGAVNALTVTETISSNNLGGLGSNGTGASVTFA
                     RSTISGNSNGLVAVNGGALVSSGGNIVVDNATPGAPTATIAPL"
     gene            5001..>5472
                     /locus_tag="VF633_05845"
     CDS             5001..>5472
                     /locus_tag="VF633_05845"
                     /inference="COORDINATES: protein motif:HMM:NF020459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HEX8470615.1"
                     /translation="MNPYEHLGAEAFWRSAIAQRNPLEISEMWAPKTAFPEGVRIATA
                     GSCFAQNIGAALRRNNLAWINTEPAPTTMPAEVRRAFGYDLFSFRTGNIYTARQLRQW
                     LRWASDAETPPIEVWARNGRFFDPFRPAIEPNGFDTPEDVHRARATTLEAIAEAI"
BASE COUNT          958 a         1987 c         1623 g          904 t
ORIGIN      
        1 catccgcttc ccggacgacg agacgatggg cgccatcgtc gaggtccact tccccggcat
       61 caggccccgc ctcgtctccg cggcgctgaa gaccttctac gagatccgcg acacgccggg
      121 tctgaagaag aagccctcga cctcggagtt gctggactgg ctcaaactcc tgctggtcga
      181 cgatatcgac ccggaaaccc tgcgggagac gtcgcccaac aagctgatcc cgcccctgca
      241 cggcgccctg ctcaagaacg aacaggacgt ccacctgttc gaacgcctcg ccttcctcac
      301 acgccgcgaa ggcggtcggc cagggtcgtg atcggggcgc cgatcttgcc gggccggaca
      361 cgggcccatc cggcacagga aggtcgggtt tcgccacgcc gattaccgcg atttcactgg
      421 cgcatcatgt cgttcagcgc aggatgaccc tgtgaaaagg actccgttga tgctccgcct
      481 gccaacatca tacctgcccg cgcctcgccc ctggtcccgt ctggccatgc tcgccgcctg
      541 cgcctccggc cttgtcctct tcgccgcttg cgacgacagc ggagtgcgca tcagccgcag
      601 cacgtcgaac agcgacgccg agggcgtgct caaggtcgtc gatgccctcc agtgccccga
      661 aagccttggc gccctcacgc gcaagggctc ggcgcaggct gatggcacgg tctgcaccta
      721 cacaggcccg cgcggcgccc aggtcgagct ccatctggtc acgctcgacg atgacggcgc
      781 cgatccgatc ctaaaggcct tcgagacccg gctgctggcc tccatgccgc acacggccgc
      841 cgacatcagc cgctctacgg cggactcggc ccgcgccgac gccaatgccg cccgtgccga
      901 tgcccaggcc gcccgggccg acgccgaagc cgccggagca gatgctcggg ccgaggccga
      961 tgcgaacccc gacagcgccc acatctccgc tcccggcatg aaggtggacg ccgaaggcga
     1021 tcgcgcttcg gtccatatgc ccggcatcga cgtcgaggcg gacggcgaca aggccgatgt
     1081 ccggatcggc ggtttcacta tccgcgccaa cgaagggaac gcctcgatca atggccgcct
     1141 taccaccggc gataacaccg actccgtcaa catcaacgcg cgcgacaacg ccgccgagat
     1201 ccgtacccgc gcgccgggcg aagccatccg ccagacctac ctcctgaccg acaaccggcc
     1261 ttcggacgcc ggctggcgca cggtcggcta cgaggcccgc ggcccgcgca cgggcccgat
     1321 cgtcgtcgcc acggtacgct cgcgtgagag caacgccgac ggtgtcttcg acgacgccaa
     1381 ggaactggtc gccctcaacg tcggggaata ggcgccgatt gcctccaagc ccgggccaaa
     1441 caccgggccg cacttgatcg cgcggcccgg atgggtcacc tgacctgccg acagcaggat
     1501 cacagacttg gccgacccta tctcgaagaa cgcacccaga gcgtggcagc gcatgctgtc
     1561 cggccgtcgg ctcgatctgc tcgacccctc gccgttcgac atcgagatcg aggacatcgc
     1621 ccacggcctc gcccgcgtcg cccgctggaa cggccagacc atcggcgaac acgccttctc
     1681 ggtcgcccag cactcctgcg tcgtcgagga gatcgcggtc cacatcaagc cggacctggc
     1741 ccccaaatgg cgcctcgccg ccctgctcca cgatgcgtcc gagtacgtca tcggcgacat
     1801 gatctcgccc ttcaaggccg cgctgggcat gagctacaag gacttcgagg cccgcctcga
     1861 gcacgccatc cacgtccgct tcggcctgcc gcccaggtcg ccggtggcca tcaagaccct
     1921 gatcaagaag gccgaccgcg cctgcgcctt cttcgaggcc acccaactgg ccggcttcga
     1981 gcgggccgag tcgctcaaga tcttcggcgc cccgcctccg ggctatgtcc tgatcatcga
     2041 ccccctgccc gcgcccatcg ctcaggcccg ctacctcgag cgctaccgcg tcctcggcga
     2101 ggccgtcggc atcatgccgg ccgccgacgc ctggcatacg gaatagccga tgtcgttcgg
     2161 gggcgagcac ggcgtgcgca tcggcctctc ggcggtcgtc atggcgttgc gggaccgccg
     2221 cctctgtgtc ctgaccaccg gcacggacga ggccacggcg ctcccgttcg gcccattcga
     2281 tcccgccaac gaccgcacct tcgaactgtc gctacgcgcc ttcatcaccg cccagaccgg
     2341 cttccggtcc ggcttcgtcg aacaactcta caccttcggc gatgcgggcc gtgaaagccc
     2401 ccgcccgacc cccggccccg agcgccagcg cgagatctcg gtcggctatc tcgccctgac
     2461 ccgcgacgcc tccgccgccg actccgccga cgcccgctgg acgcccgtct tcgatgcgtt
     2521 cccgtgggaa gatcgccgcg accccaccga tgccatccgc cccctgatcg ccgaagccct
     2581 gacccgttgg gccggctccg acccccgccg tctcgcccgc atcgacggcc tgttcgccct
     2641 cggccccggc ctgcgctgga acgaggaacg ggtgctcgaa cgctacgagc tcctctacga
     2701 agccggactg gtccccgaag ccgcccgcga cggcgtctgc cccgtcgcca ccgccctccc
     2761 cggcctcacg atggcctccg accaccgccg catcctcgcc accggcctcg gccgcctgcg
     2821 ctccaagctg aagtaccgcc cggtcctgtt cgaactggcc cccgagacct ttaccctgtc
     2881 cgaactccag accgccgccg aggccgtcgc aggcctcccc ctgcacaagc agaacttccg
     2941 ccgcggcgtc gaacgcaccg gtctcgtcga acccacaggc cgcctcagcg ccgccaccgg
     3001 aggccgcccc gccgagctct tccgcttcac cggcgccgac ccccggggcg gtcaagcccc
     3061 cggcctcgcc ctccctgccc agcgctgatc cggagcttga cgaaaatcgt cagtaagccg
     3121 cttgcagcgc ccgccctgtc cgactagaga agcccctcgc tcgaccaagc gtactacggc
     3181 accgcggaga ggtggcagag tggtcgaatg taccgcactc gaaatgcggc gtgcctggaa
     3241 gggtaccgtg ggttcgaatc ccaccctctc cgccagccat tacgctgata actagcggtc
     3301 tccctaccct caggaaatta tcgcgacatt gctgcgacat ccaggatccg ggagaccggc
     3361 tcaccgtctc cgatcgtgtg aaaacgccaa gcttctcaag cactcgcacc tgactcaacc
     3421 cgccaacctg ataaacgctg accctgtcca tcctcatgct gcggtcgccg atccaagccc
     3481 agtttcggga cggaacttac gaaggttcct gcggagtctt tgtcgcggcg gcggctgcga
     3541 caaaggctga taggcgctat gactcgctgg cgtgaaagtc ccggaacgtt gcctatcacc
     3601 ctagccgcag ccactgctca gagcggcgcg atagtcgcgg tcggcgcacc tggagtggcg
     3661 ttgtcgacga caatgttgcc cccgctggaa accaaggccc ctccgtttac ggcgacgagg
     3721 ccattgctgt tgcccgagat cgtagagcgg gcaaacgtga cgctcgcgcc cgtaccgttg
     3781 gaccccaaac cgccaagatt attggaggaa atggtttcag tgacagtcaa agcgttaact
     3841 gcccccaagc tgatcgccag gacaccagcg agcgagttgc cggaaatcag actgccgctg
     3901 atcgaagtat gcaccgcgcc cgctgtggcg ctgctgtcga tgacgacgcc ccgggtattg
     3961 ttcaggatgc ggctatttcg aatggacacg aaggcggagc ccgagcctgt aggagcgatc
     4021 tggatggcgg agccagtcga tcccgtccct gactcgctta tcgtcgtgtc ggcgatgttc
     4081 acataagtcg tacccgcgct gtttagcagg gcgacaccgt agccggcgct gggggatcgg
     4141 gcgcccatta ttgcgctgtt ctgaatgttg actgtgcccg ccgccaggat gcgcacgccg
     4201 ttgaggccgg ttgaggcccc gatactgccg ttgatgtaaa gcccccgcag ggtgaccacg
     4261 tcagtcgcac cggcgttgat tgtaacgcca gtggtggaag aggccagtat tccgccgaag
     4321 ccggggtcag tcgtaatcgt gatcgacttg gtgatcgtga ccgtgccaaa accgcccgga
     4381 tcaagcacgt ttatctcacc ccccgccgca gtctttgaaa ttgcgccggc gaacgtcttg
     4441 cagggcgcgg ttcggctgca cgggttaacg tcgtcgccca ctccggaaac ccaggttcgg
     4501 gttgcctgag cgctggatat gccaggggcc gcaaaacagg caagcgtcaa gccgacaagc
     4561 aacaggtgtt tcgtcatgat cttccccaga aggagtttgc tcgaccgatg gtcgacgagc
     4621 gtcggtctct agggataacc ttcgctggat ttcttgcaag agtgcttttc tcgccaccgg
     4681 ggccgaagat gttggtctgg tcgccaaccg cggtcctcgg gatcgcgcgt gcgctgggct
     4741 tggacggtgc agggcgagtg cagaggccgc atgaaggcct aggtcgcttt gctgggcacg
     4801 ctgagcgcct gcctatgcca caactaaccc tagagccggc atggggctca gcaacttccc
     4861 ccgacgcaca agaacgttga tcgccggacg ccaatcacct atcgttgatc gccccagctt
     4921 taccgcgagg ctccgcacag cacgctgctg ccagcagatt ttgcgccttc gggcctatgg
     4981 ttgggaagag tcgtcactcc ttgaacccgt atgagcatct cggcgcggaa gcattctggc
     5041 gatcagcaat cgcccaaagg aacccgcttg agatttcgga gatgtgggcg ccgaaaacag
     5101 cgtttccaga gggcgtgcgg atagcaaccg ccggctcctg tttcgcgcag aacatcgggg
     5161 cggccttgcg tcggaacaac cttgcctgga tcaacaccga gccggcgcca acaaccatgc
     5221 ctgcggaggt ccgccgcgcc ttcggttatg atctattctc attccgaacc ggcaatatct
     5281 acaccgctcg acagttgcgg cagtggcttc gctgggcgag cgacgccgag acgccgccca
     5341 tagaagtgtg ggcgcgcaat ggaaggtttt ttgatccgtt ccgtcccgcg atcgagccga
     5401 acggttttga cacgcccgag gacgttcacc gtgcccgggc caccacgctg gaagcaatcg
     5461 ctgaagccat ct
//
LOCUS       DASVTM010000218         4827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_870519,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000218 DASVTM010000000
VERSION     DASVTM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870519"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..791)
                     /locus_tag="VF633_05850"
     CDS             complement(183..791)
                     /locus_tag="VF633_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8470616.1"
                     /translation="MKIVVLGASGRAGSEITRELASRGHSITAVARKADAIAPAPGVT
                     AVQGDASNATALAQLIKGSDVVVSALHHDVTAATLLTALKAAGVPRLLVTGGAGSLEV
                     APGVRLIDTPAFPEEFKPYALPGIAFLDDLRGETDIDWTFFSPAALIEEGPRLAQFRL
                     GTDQLITDGKGDSRISFADYAIAMADEVEQHKHPRARFTAAY"
     gene            845..1285
                     /locus_tag="VF633_05855"
     CDS             845..1285
                     /locus_tag="VF633_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8470617.1"
                     /translation="MQEGTASTSGHLQETCPTPGETNWEGDVYARNCPTRLLLDRIAD
                     KWTVLLLTTLKDEPMRFNALKRRVEGVSQKMLSQTLRQLERDGLVTRSAEATVPVSVT
                     YAITPLGETLVLALQPIVAWAESRMPMVAEARATFDRGITLTQA"
     gene            1375..2022
                     /locus_tag="VF633_05860"
     CDS             1375..2022
                     /locus_tag="VF633_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEX8470618.1"
                     /translation="MLGWFQALLPKEDNFFRLFEAHAATLTKGAEAMRHMLDGGDAVP
                     GWCAKIVEHENEADDVAREVLVAVRRSFITPFDRSDIRGLTNSLDDAIDQMQKTAKCV
                     TLYDLREFTAPMRELGDIAVRSAVLTVEAVSLLPAMTKHSDRLNSLTEQLSRLEDESD
                     LLYDQGMKALYAEQKNAGSALDFIIGAEVYDHLEKVIDRFEDVATRINGILIEHL"
     gene            2024..3025
                     /locus_tag="VF633_05865"
     CDS             2024..3025
                     /locus_tag="VF633_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX8470619.1"
                     /translation="MDHALLILVFLIGVALLFDFLNGLHDAANSIATIVATRVLPPVY
                     AVGWAAFFNFIAFLFFGLHVANTIGKGIIDPAIISDQVIFGALMGAIAWNVVTWLAGI
                     PSSSSHALVGGLLGAGIAKAGLGAVVLSGTLKTVGAIFLSPAVGFALALLLVVIVSWL
                     FAKSNPAFADKTFRRLQFISASAYSLGHGGNDAQKTMGIIAVLLLSRAPAGTEFHVPL
                     WVVLSCHSAIALGTLSGGWRIVRTMGSKITRLSPQQGFCAETGGAITLFLATGLGIPV
                     STTHTITGAIVGVGAARRVSAVRWGVARSIVFAWFVTIPAAAAVAAVAYHIGGLFLT"
     gene            3118..3474
                     /locus_tag="VF633_05870"
     CDS             3118..3474
                     /locus_tag="VF633_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8470620.1"
                     /translation="MITSRETRRWVIPKGGRMAGKSDSQAAAIEAMEEAGVQGEIGET
                     AIGAFRYAKRLKNGDERMCIVAVFPLKVLIQLGAWPEVAERERRWMLVEDAAASVMEP
                     DLADLIQAFGTADIED"
     gene            3803..4318
                     /gene="rplJ"
                     /locus_tag="VF633_05875"
     CDS             3803..4318
                     /gene="rplJ"
                     /locus_tag="VF633_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX8470621.1"
                     /translation="MDRAQKAESIETLKGVFADAGAVVVTHNLGLTVAEMEDLRGRLR
                     TEGGAFKVVKNRLALKALDAAEGSAHHALFKGPVGIAYAPDAVTAAKVTADFAKGNDK
                     FVVLGGFMGETILDAAGVSTLSKLPSLDQIRASFLGLLNTPATRIAGIVQAPAAQLAR
                     VFNAYATKDAA"
     gene            4381..4764
                     /gene="rplL"
                     /locus_tag="VF633_05880"
     CDS             4381..4764
                     /gene="rplL"
                     /locus_tag="VF633_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX8470622.1"
                     /translation="MADLSKIVEDLSSLTVLEAAELSKLLEEKWGVSAAAPVAVAAAG
                     GGAAAPAEAAEEQTEFTVVLLDGGDKKINVIKEVRGVRSDLGLKEAKDLVEAAPTNVV
                     ENVSKQVAEEVKKKLEEAGAKIQIK"
BASE COUNT          881 a         1457 c         1573 g          916 t
ORIGIN      
        1 actgatgcgc gatatgggca acacaatgtg caaagagctg agttgcactg cgcaagagcg
       61 tacgcagatg caagaagcta tggctcaggc aggctatgga acgcctcaac tgtccaaata
      121 agcccgtcag cttggcgcct accggtgggc gagttgctgc ccaccggtac gccctctctc
      181 actcagtacg ccgcagtgaa gcgggcgcgg gggtgtttgt gctgctcgac ctcgtcggcc
      241 atggcgatgg cgtagtccgc gaagctgatg cggctgtcgc ccttaccgtc ggtgatcagt
      301 tgatcggtcc cgaggcggaa ttgggcgagg cgcgggcctt cctcgatgag ggctgcgggt
      361 gagaagaatg tccagtcgat gtcggtctcg ccgcgaaggt cgtcgaggaa ggcaatgccg
      421 ggcaacgcgt acggcttgaa ctcttcgggg aaggctgggg tgtcgatcag gcggaccccc
      481 ggtgcgacct caaggctgcc tgcgccgccg gtgaccagca ggcgagggac gccggctgcc
      541 tttagcgcgg tcagcagggt ggcggcagtg acgtcatgat gcagggcgct gacgaccaca
      601 tccgaaccct tgatgagctg ggcgagtgcg gtcgcgttcg acgcgtcgcc ttgaacggcg
      661 gtgacacccg gagccggggc gatggcgtca gccttgcggg caacggcggt gatcgaatgg
      721 ccgcgcgacg cgagctccct ggtaatttcc gagccggcgc gaccgctggc gccgaggact
      781 acgatcttca tggattgagc tccgtggttt ccaatagtga ccaagtgcga tacggagatc
      841 ggctatgcaa gaaggcaccg cgagcacatc tggtcacctg caggaaacct gccccacacc
      901 cggcgagacc aactgggagg gcgatgtcta tgcgaggaac tgtccgaccc gtctgctgct
      961 ggaccggatc gcggacaagt ggacggtgct gttgctgacg acgctcaagg atgagccgat
     1021 gcgtttcaat gcgctgaaac gtcgggtgga gggggtgtcg cagaagatgc tcagccagac
     1081 cctgcgccag ctcgagcggg acgggctggt tacgcggtcc gccgaggcga ccgtgccggt
     1141 ttcggtgacc tatgcgataa cgccgctggg tgaaacactg gtgctggcgt tgcagccgat
     1201 cgtcgcctgg gctgaaagcc gcatgccgat ggtcgctgaa gcccgggcga cgttcgaccg
     1261 cggaatcacg ctgacgcaag cgtgagcgac gaggcggctt gacctccggc gggggatggc
     1321 gttttgaggc gaaagattgt cggccgatgg ctggcgacag acgggggata tccgatgctc
     1381 ggctggtttc aggcgctgct gcccaaggaa gacaattttt tccgtctgtt cgaggcccac
     1441 gccgcgaccc tgaccaaggg agcggaggcg atgcgccata tgctggacgg cggcgacgcg
     1501 gttccgggct ggtgcgccaa gatcgtcgaa catgaaaacg aggccgacga cgtcgcccgt
     1561 gaagtgctgg tcgccgtgcg gcgcagcttc atcacgccgt tcgatcgctc ggatatccgc
     1621 ggcctgacca actcgctaga cgatgccatc gaccagatgc agaagaccgc caaatgcgtg
     1681 accctgtacg atctgcgcga gttcacggcg ccgatgcgcg agcttggaga tatcgccgtc
     1741 cgttcggctg tgctgaccgt ggaggctgtg tccctgctgc cggccatgac gaaacacagc
     1801 gaccggctga acagcctgac cgagcaactg tcccggctcg aggacgagag cgaccttctc
     1861 tatgatcagg gcatgaaggc gctctatgcc gagcagaaga acgcaggcag cgcgctcgac
     1921 ttcatcattg gcgccgaggt ctacgatcat ctggagaagg tgatcgaccg gttcgaggac
     1981 gtggcgacgc gcatcaacgg cattctaatc gagcacctgt agggtggatc acgccctcct
     2041 gatcctggtc ttcctgatcg gtgtcgccct gctgttcgac tttctgaacg gcctgcatga
     2101 cgccgccaac tccatcgcca ccatcgtggc gacgcgggtg ctgccgcccg tctatgcggt
     2161 gggctgggcc gcgttcttca acttcatcgc cttcctgttc ttcggcctgc atgtggccaa
     2221 caccatcggc aaaggcatca tcgatccggc tatcatctcg gatcaggtga ttttcggcgc
     2281 cctgatggga gccatcgcct ggaacgtcgt gacgtggttg gcaggcattc cttcgtcgag
     2341 ctcgcacgct ctggtaggcg gcctgttggg cgcaggcatc gccaaggcgg ggctcggggc
     2401 cgtcgtgctg tcgggcacgc tgaagacggt cggcgccatc ttcctgtcgc cggccgtcgg
     2461 gtttgcgctt gccctgttgc tggtggtcat agtgtcctgg ctgttcgcga agtctaatcc
     2521 ggctttcgcc gacaagacgt tccgcaggct gcagttcata tccgcctcag cttattcgtt
     2581 ggggcacgga ggaaatgacg cacagaaaac catgggaatc atcgccgtcc tgttgctgtc
     2641 gcgggctccg gcaggtaccg aattccatgt gccgttatgg gtcgttctgt cctgtcactc
     2701 ggcaatcgct ctcggaacct tgtccggggg ctggaggatc gttcgcacga tggggtcaaa
     2761 gattacccgc ctgtcgccgc agcaaggctt ctgcgccgag acgggcggag ccatcacctt
     2821 gttcctcgcc acgggtctgg gcatccccgt ttcgaccacc cacaccatca ccggcgctat
     2881 cgtcggcgta ggcgcagccc gtcgggtctc ggccgtgcgc tggggcgtgg cgcgtagcat
     2941 cgtcttcgcc tggttcgtca ccatcccggc cgccgctgcc gtcgccgccg tcgcctatca
     3001 tatcggcggg ttgttcctga cgtgaccggt aaggcggtca aacgatccgc cagatccgag
     3061 acacgacagg tcgctgccct gccgtggcga accggtgcga gcggtcttga aatcctgatg
     3121 atcacctcgc gcgagacgcg ccgctgggtc attcccaagg gcggacggat ggcggggaaa
     3181 agcgattcac aggccgccgc tatcgaggcg atggaggaag cgggcgtcca gggcgagatt
     3241 ggagagacgg caatcggagc cttccgctat gccaagcgtc tcaagaacgg cgacgaacgg
     3301 atgtgcatcg ttgcggtgtt cccgttgaaa gttctgatcc agctcggcgc ctggcccgaa
     3361 gttgctgaac gcgagcgtcg ctggatgttg gttgaggatg cggcggcctc ggtcatggag
     3421 ccagacctcg ccgacctgat ccaagcgttt ggaaccgccg acatcgagga ctagccattc
     3481 ggccctgtca ggggagcttg ctttctacgc gcgtcacctg taagagcgcc gcttctccgt
     3541 cgaggccttc tggtttcggc ggtcctgtcc gagaacttcg gggcgtgggg gtcacccatg
     3601 ccataactgt cagagggccc tctgacaccg tggggagatg gggacgcgaa cgacagccgt
     3661 cagcccgcac gtcgggacga cagcgttagt tgcatccttc ggacattacg accggctcga
     3721 acgcttcgca gcgatgcgtg gcgctttggc catatcgtca tcgggaataa gcccgttggc
     3781 gaactctaga ctggagaccg caatggaccg cgcacaaaag gccgagtcga tcgaaacgct
     3841 gaaaggcgtt ttcgctgacg ccggcgccgt ggtcgtgacc cacaacctgg gtctgaccgt
     3901 tgcggaaatg gaagatctgc gcggccgtct tcgtaccgaa ggcggcgcat tcaaggtggt
     3961 caagaaccgc ctcgcgctga aggctctcga tgctgccgaa ggttccgctc accatgccct
     4021 gttcaagggc ccggtgggta tcgcctacgc acccgacgcc gtcaccgccg ccaaggtcac
     4081 cgctgatttc gccaagggca acgacaagtt cgtcgttctg ggtggtttca tgggcgagac
     4141 cattctggac gccgccggcg tttcgaccct gtcgaagctg ccgtcgctgg atcagatccg
     4201 cgcttcgttc ctcggcctgc tcaacacgcc tgcgacccgt atcgccggca tcgttcaggc
     4261 tccggccgcc caactggcac gggtgttcaa cgcctacgcc acgaaggacg ccgcgtaagc
     4321 gaaccgactt ccatctctgc aaatctctac tgaacccaat cctccgaagg aaaactgact
     4381 atggctgacc tgtccaagat cgtcgaagac ctgtcctcgc tgaccgtcct cgaagctgcc
     4441 gaactgtcca agctgctcga agaaaaatgg ggcgtctcgg ctgccgctcc ggttgccgtt
     4501 gccgctgccg gcggtggcgc tgccgctcct gctgaagctg ctgaagaaca aaccgaattc
     4561 accgtcgtcc tgctggacgg tggcgacaag aagatcaacg tgatcaagga agtccgtggc
     4621 gtacgttcgg acctcggtct gaaagaagcc aaggatctgg ttgaagccgc tccgaccaac
     4681 gtcgtcgaga acgtctccaa gcaagtcgct gaagaagtta agaagaagct ggaagaagcc
     4741 ggcgccaaga tccaaatcaa gtaatttggg attggtcctc gcccctcggg gcgaggctat
     4801 cttcaggcag gcccgatggg aaaccat
//
LOCUS       DASVTM010000219         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_181097,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000219 DASVTM010000000
VERSION     DASVTM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181097"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VF633_05885"
     CDS             complement(<1..1323)
                     /locus_tag="VF633_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8470623.1"
                     /translation="MPIRKRLLGFAFASADLLIELSADGRVAMALGSGPAAGVGPEAF
                     QGLPLIDRLAPGGGKILMKALAGLKPGARTGPVELLLACGDGRVRRATLRAFMLPELA
                     PGISCSIIYDGPVFSIASAESPPILTAAAFLERAQGVITDTDSGDLAISFIDIIGLNE
                     AGSAGARATARVEAALQFASSDGACATRLTPERYALLHDNEDARDMIGEVRELIHDEG
                     LDLKVSGSSTPIPDCPPVNALRALRFALEACMADGGLERPDLFFKAALSRTMEEAESF
                     RAMVKARDFELHYQPIVDLKTGAVHHFEALSRFNTEVSPFNTIRMAEELALIEGFDLA
                     VVEKALQRLRKPGSGLLRIAVNVSGASLGSDTYVDSVLRMTSAAPDERRRLIVEVTES
                     AALADIAAANRRLHAFRDAGIKLCIDDFGAGAASFDYLHGLSVDTVKID"
     gene            complement(1414..1851)
                     /locus_tag="VF633_05890"
     CDS             complement(1414..1851)
                     /locus_tag="VF633_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body protein FlbY"
                     /protein_id="HEX8470624.1"
                     /translation="MSDQKTATDNARRLKALTERLTERLVVESRAFAERRPRDVLDGM
                     IETQELANQYRRESAQVKANPSLLSACQASERMALVKATERFNQVLAGHARAVEAARI
                     ISEGLVQTIAGEVAASRAMGTGYGASGRAAPGDGRAITLNRSA"
     gene            complement(1848..2162)
                     /locus_tag="VF633_05895"
     CDS             complement(1848..2162)
                     /locus_tag="VF633_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEX8470625.1"
                     /translation="MSDLATPVSLLSPVPTAPGANLSPAALDRIKKTAQDFEASFLSE
                     MYKPMFQGLSTDGPFGGGEAEGTWRSFLIDAMAKQTVKAGGVGLSNVVMTEMIKMQEG
                     RQ"
     gene            complement(2164..>3114)
                     /locus_tag="VF633_05900"
     CDS             complement(2164..>3114)
                     /locus_tag="VF633_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX8470626.1"
                     /translation="GTGDSLRNCPFTRQSLEGMTERLGVNIRGANANSKNMAAIMVTA
                     ELPPFSTPGARIDVAVSSMCDAKSLLGGTLLVTSLQGADGEVYAVAQGSVQTGSVSGS
                     GGSGSSVTRGVPTAGRIASGATVERETGFNLDSMPEVRLTLRNPDFTTAQRIAAAINQ
                     SFPQTALAENGSVVALRAPAQFGMAGFISRLENLQIAVDAPAKVIIDEVNGVIVMGEA
                     VRVSTVAIAQGNLTISVQEDPQVSQPAPFSQGQTAVVPSSTVNVEEELGREMRIVNGS
                     TSLSTLVNGLNALGVSPRDMISILQAIKSAGALQAEIEVM"
BASE COUNT          564 a          961 c         1030 g          559 t
ORIGIN      
        1 gtctatcttc acagtatcga ccgacaagcc gtgaaggtaa tcgaacgaag cagcgccggc
       61 tccgaaatcg tcgatgcaga gcttgattcc tgcgtcacga aacgcgtgca agcgacggtt
      121 tgctgcggcg atgtcggcca gagctgcgga ctccgtcact tcgacgatca gtcggcgtcg
      181 ctcgtccggc gccgcggacg tcatgcgcag gactgagtcg acgtaggtat cgctgcccag
      241 ggaggcgccg gagacgttga cggcgatgcg taacaaaccc gacccgggtt tgcgcagccg
      301 ctgcagggcc ttctcgacga cggcaagatc gaagccctcg atcaaggcta attcctctgc
      361 catgcgaatg gtattgaagg ggctgacctc tgtgttgaaa cgggacagag cttcgaaatg
      421 gtgtactgcg cccgtcttca gatcgacgat cggttggtag tgcagttcga aatctcgcgc
      481 cttgaccatg gcgcggaaag actctgcttc ctccattgtc cggctgaggg ccgccttgaa
      541 gaacagatcg gggcgttcca gtcccccgtc agccatgcag gcttcgaggg cgaaacgcag
      601 ggcccgcaga gcgttgaccg gagggcaatc cgggattggc gtggaggatc ccgagacctt
      661 gaggtcgagc ccttcatcgt ggatcagttc ccgaacttca ccgatcatat cccttgcgtc
      721 ttcattgtcg tgcagcaagg cgtagcgttc aggtgtcagg cgggtcgcgc aagcaccgtc
      781 ggaggaagcg aactgcagag cagcctcaac tctcgccgtc gcgcgcgctc ctgcagatcc
      841 agcctcattc agcccgatga tgtcgatgaa ggaaatcgca aggtcacccg agtccgtgtc
      901 agtgatcacc ccctgcgcac gctcgagaaa agcggctgcg gtgaggatgg gcgggctctc
      961 cgccgaagcg atcgagaaaa ccggaccgtc gtagatgatc gagcaggaga ttccgggcgc
     1021 cagctccggc agcatgaaag cacgcaaggt tgctcgacgc accctgccgt cgccacaggc
     1081 cagcagcagt tccaccggcc cggttcgcgc gcccggcttg aggccggcaa gggctttcat
     1141 gaggatcttg ccgcctccgg gcgcaagccg gtcgattaga ggtagtccct ggaaggcctc
     1201 cgggccgacg ccggccgcag gaccggagcc gagcgccata gcaacacgcc catccgccga
     1261 cagctcgatc agcaggtcgg cggaggcgaa ggcgaagccc agaagtcgct tacggatcgg
     1321 catgaccgga ccgtgcactg aaaggcctta aagcttcgcc ccattgcacg gttaacggcg
     1381 ttcaatctca gcgcaacggc aggattatgt gggtcaggcg ctgcgattga gagtgatcgc
     1441 gcgtccatcg cccggggcgg cgcggcccga cgctccgtat cccgtaccca tagcccgcga
     1501 ggctgcgact tcgcccgcga tggtctgaac caggccttcc gagatgatgc gagctgcttc
     1561 gacagcgcgg gcgtggccgg ccaacacctg gttgaatcgc tcggtcgcct tcaccaaggc
     1621 catgcgttcg ctggcttggc aggctgagag tagtgagggg ttggccttca cctgggccga
     1681 ctcccgacgg tactggttcg ccaactcctg ggtctcgatc attccgtcca gaacatctct
     1741 cggacggcgc tcggcaaaag cgcggctctc gaccaccagt cgctcggtca agcgttcggt
     1801 cagagctttc aaacgacggg cattgtccgt ggcggtcttc tgatcgctca ttggcgcccc
     1861 tcctgcatct tgatcatttc ggtcatcacg acgttggaca ggccgacgcc gccggccttg
     1921 actgtctgtt tggccatggc gtcgatcagg aagctgcgcc atgtgccttc ggcctcaccg
     1981 ccgccgaagg gtccgtcagt cgacaggccc tggaacatcg gcttgtacat ctcggacagg
     2041 aaggacgcct cgaagtcctg agcggtcttc ttgatccggt ccagagctgc cgggctcagg
     2101 tttgcgccgg gcgcggtcgg aaccggcgac agaaggctga cgggagtggc gagatcgctc
     2161 atgtcacata acctcgattt cggcttgcag ggcgccggcg gacttgatgg cctgaaggat
     2221 ggagatcatg tctcgcggcg aaacgcccaa ggcattcagg ccgttgacca gtgtcgagag
     2281 agaggtcgag ccgttgacga tccgcatctc ccgcccaagc tcctcttcaa cgttgacggt
     2341 ggaggagggg acgacggcgg tctggccctg gctgaacggc gcgggctgac tgacctgcgg
     2401 gtcttcctgc accgagatgg tcaggttgcc ctgagcgatg gcgacggtgg aaacacggac
     2461 ggcctcgccc atgacgatga cgccattgac ctcgtcgatg atcaccttgg ccggagcatc
     2521 gaccgcgatc tgcaggttct cgagccgact gatgaagcct gccattccga attgcgccgg
     2581 ggcgcgcagg gcgacgaccg aaccgttctc ggcaagggcg gtctggggga aggactggtt
     2641 gatggcggcg gcgatccgct gggccgtagt gaagtccggg ttgcgcaagg tcaggcgaac
     2701 ttcgggcatc gagtcgaggt tgaagccggt ctcgcgctcg acggtcgcgc cggatgcgat
     2761 gcgacccgcc gtcggcacgc cgcgcgtcac cgatgatccc gaaccgccgg agccggagac
     2821 ggatcccgtc tgaacggagc cctgggccac ggcatagacc tcgccatctg cgccctgcag
     2881 gctggtgacc agcagggtcc cgcccagcag gctctttgcg tcgcacatgc tggacaccgc
     2941 gacatcgatg cgggcgccgg gggtcgagaa gggcggcagt tctgcggtca ccatgatggc
     3001 ggccatgttc ttggagttcg cgttggcgcc ccggatgttg acgccgagac gctcggtcat
     3061 gccttcgagc gactgacggg tgaacgggca gttgcgcagg ctgtcgccgg tgcc
//
LOCUS       DASVTM010000220         8676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_391727,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000220 DASVTM010000000
VERSION     DASVTM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391727"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="fmt"
                     /locus_tag="VF633_05905"
     CDS             complement(<1..724)
                     /gene="fmt"
                     /locus_tag="VF633_05905"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX8470627.1"
                     /translation="MRLAFMGTPEFAVPSLAELIASGHEIVAVYSQPPRPKGRGQKLT
                     PSPVHAFAEAMGLPVFTPESMKSADGIEIFQSLDLDAACVVAYGQILKPAVLEAPRLG
                     CFNLHGSLLPRWRGAAPIQRAIMAGDRETGVQIMRMSEGLDEGAVLLGEVLPIHPEDT
                     AATLAERMSVTGATLWTRALAAIERGGVTETPQAGEPSYARKITPGEARIDWTRTATE
                     VDCHIRGLSPFPGAWFAGPDGTR"
     gene            complement(877..1092)
                     /locus_tag="VF633_05910"
     CDS             complement(877..1092)
                     /locus_tag="VF633_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470628.1"
                     /translation="MMGRSTVIDTEGRRRLTRSEKIGIGSLATLVGVAAIGLIAGVLL
                     DRLLDFDDALDAGGEWDEGGGVASRWE"
     gene            complement(1089..1682)
                     /locus_tag="VF633_05915"
     CDS             complement(1089..1682)
                     /locus_tag="VF633_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX8470629.1"
                     /translation="MTVRRILTIDNAADLAVLKQISKPVEAVDDDVRALMDDMLETMY
                     AAPGIGLAAVQIGDLRRVVVMDLHDGDLPEGDVQKVDEAGAEVEDDRRSNPRFFVNPE
                     VLWSSDELFQYEEGCLSLPEYFDTVERPARIRVAYLNREGQRVEEEIDGLYAVCFQHE
                     LDHLEGVLFIDHLSRLKRDRAMSKVKKYMRSLALEDA"
     gene            complement(1740..2159)
                     /locus_tag="VF633_05920"
     CDS             complement(1740..2159)
                     /locus_tag="VF633_05920"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX8470630.1"
                     /translation="MSDTPPETPLQAPDQQPVVRVIAMPADTNPEGDIFGGWLLAQMD
                     LAGSTPAFDLARGRCATIAVDAMVFHQPVSVGDEVSIYARVLRVGRTSIRVHVEAWKR
                     PRASHHISRVTEGVFTYVAIDEHRKPRALPEDPSAAE"
     gene            complement(2161..2919)
                     /gene="ccmB"
                     /locus_tag="VF633_05925"
     CDS             complement(2161..2919)
                     /gene="ccmB"
                     /locus_tag="VF633_05925"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX8470631.1"
                     /translation="MSAGGPKFTKWNEAKPETPEPGAVEPHAPGLRGATRVLFERELS
                     LAWGGGGGPLLACAFFACLTAILPLAAGGDLKTLGPVAAGVAWLALALSSLLSLERLF
                     ERDLEDGALDLLATGHLPLESVFVIKALSQWIATGLPLALTAPVAALALGQPIHLAPL
                     TALAAGLGGLGFAFTGALGAALALGAKRGGLLIAVVVLPLFIPPVVFGSGALARAVQG
                     LSPGPALALLAAYALFAAVIAPFAGAAAIRNAQG"
     gene            complement(2916..3539)
                     /gene="ccmA"
                     /locus_tag="VF633_05930"
     CDS             complement(2916..3539)
                     /gene="ccmA"
                     /locus_tag="VF633_05930"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX8470632.1"
                     /translation="MIHSLSLQGLSIVRGERVLFRDLDFVLKAGEVVALTGANGAGKT
                     SLLRAVAGLIRSEAGEITFRDGDHNVLEAREARGREVHLLGHLDGLKGARTAREELAF
                     QCDWLGHTQDGVDDAIETFGLKPLLDLEVRKLSAGQRRRLALGRLVGSPRALWLLDEP
                     MAPLDARWRSAFAEAMQRHLRADGLIIAAVHDALPFPARALDLGGLT"
     gene            3729..6473
                     /gene="acnA"
                     /locus_tag="VF633_05935"
     CDS             3729..6473
                     /gene="acnA"
                     /locus_tag="VF633_05935"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX8470633.1"
                     /translation="MPSIDSLKTRQDLSVGRKKYAYYSLPAAEEAGLTGISRLPRSMK
                     VLLENLLRNEDGVSVTEDDLKAVAAWIENKGSVEHEIAFRPARVLMQDFTGVPAVVDL
                     AAMRDAMTALGADASKINPLNPVDLVIDHSVMVDHFGTSGAFGQNVEREYERNIERYN
                     FLRWGSSAFNNFRVVPPGTGICHQVNLENLAQTVWTLDEGKKTVAYPDTVVGTDSHTT
                     MINGLAVLGWGVGGIEAEAAMLGQPIPMLIPEVIGFRLSGAMPEGTTATDLVLTVTQM
                     LRKKGVVGKFVEFFGPALPNMTIEDQATIANMAPEYGATCGFFPVSAATIAYLTATGR
                     DKARVALVEAYAKAQGLWIDETSEDPVFTDVLELDISTVVPSLAGPKRPQDKVELTIA
                     APSFETALTDIFNRPSDAPRYAVDGEKFTVGDGDVVIAAITSCTNTSNPSVLIAAGLV
                     ARKAHKLGMKVKPWVKTSLAPGSQVVTDYLTDAGLQKDLDALGFNLVGYGCTTCIGNS
                     GPLAPAISKSINDNAIVATSVLSGNRNFEGRVNPDVQANYLASPPLVVAYALAGSMRI
                     DITKDPIGQDKKGNDVFLKDIWPTTAEIAVIQKKSVTPAMFAKRYADVFKGDKHWQAI
                     KVTGGQNYAWDDSSTYVQNPPYFEGLSMEAKPVTDIVEGRVLAIFGDSITTDHISPAG
                     SIKTSSPAGAYLVNHGVDPLDFNGYGARRGNHQVMMRGTFANIRIRNKITSDIEGGVT
                     KHFPSGDTMSIYDAAMRYQSEGRPLVVFAGKEYGTGSSRDWAAKGTKLLGVRAVIAES
                     YERIHRSNLVGMGVVPLQFKADGWQKLGLTGEEIVTVRGLSDVNIGKLRPRQDLWVEL
                     FRPSDGKMARFPVRCRIDNQTELDYFKAGGVMPYVLRNLAAGAVAPTPEPETEVLAAE
                     "
     gene            complement(6744..7412)
                     /locus_tag="VF633_05940"
     CDS             complement(6744..7412)
                     /locus_tag="VF633_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8470634.1"
                     /translation="MITLHAFGRVHPFVHGETRDLRIQWALEEMGLPYKVRGWDHTGG
                     ETKGPEFSAISPFNQIPVLTDGDVTISESAAILIHLAEKSGCLIPADAEGRAHVIQWC
                     LAALATVEGPIQPLKLMNMGAMGDPKSANGDARAFLMDWTHRVLDGVNRRLEGRDWIA
                     TDAFTIADLLLAAVLRQLRQTNLMDGYPNVKAFYVCAMSRPAWQRTLDLYAERLGADR
                     AAIE"
     gene            complement(7475..>8676)
                     /locus_tag="VF633_05945"
     CDS             complement(7475..>8676)
                     /locus_tag="VF633_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX8470635.1"
                     /translation="PNAKRETVYLRDLLASAEYAKPAHILPLALGESIGGEPYVADLA
                     RMPHLLIAGTTGSGKSVGVNAMILSILYRLSPSECRFIMIDPKMLELSVYDGIPHLLA
                     PVVTDPKKAVVALKWVVREMEDRYRRMSKLGVRNVASYNDRAREAQAKGEHFERTVQT
                     GFDDQGRPVYESEKIRPEPLPYLVVVMDEMADLMLVAGKDVEGAVQRLAQMARAAGIH
                     LIMATQRPSVDVITGTIKANFPTRISFQVTSKIDSRTILGEQGGEQLLGQGDMLYMAG
                     GGRITRLHGPFVTDAEVEAVCAHLRSQAEPDYLDLITDDPDGDGDNAVGGDDGGGSSG
                     DDLYDRAVAVVTRDRKASTSYVQRRLQIGYNRAASLIERMEQEGVVSQANHSGKRDVL
                     AGPPPMA"
BASE COUNT         1566 a         2886 c         2796 g         1428 t
ORIGIN      
        1 cgcgtgttcc gtcgggaccc gcgaaccacg ctccggggaa gggcgacaga ccccggatgt
       61 ggcaatcgac ctcggtcgcc gtccgggtcc agtcgatgcg cgcttcgccg ggcgtgatct
      121 tgcgcgcata gctaggttcc cccgcctgag gcgtctcggt gacgccgcca cgctcgatgg
      181 cggccaaggc gcgcgtccat aaggtcgcgc cggtgacgga catacgctcg gccaatgtgg
      241 cggcggtgtc ctcggggtgg atgggcaaga cttcgcccaa caggaccgca ccctcgtcca
      301 gaccttcgct catccgcatg atctggacgc cggtctcgcg atcgccggcc atgatggcgc
      361 gctggattgg agccgcgccc cgccaacggg gcagcaacga gccgtgcaga ttgaagcagc
      421 cgagacgcgg cgcctcgagc acggcgggct tgaggatttg gccgtaggcg acgacgcagg
      481 cggcatcgag gtcgaggctc tggaatattt caatcccatc ggccgacttc atcgattcag
      541 gcgtgaagac cggcaggccc atggcctcag cgaacgcatg cacgggcgac ggcgtcagct
      601 tctggcctcg ccctttggga cggggcggct gggaatagac ggcgacgatc tcgtgacccg
      661 acgcgatcag ctcagccaac gaaggcacgg cgaattcggg agtgcccatg aaggccaggc
      721 gcatgtttgt tctttctttt ccgcctgacg gcgggagcgc tatcgctcgg ctcgcggagc
      781 ctcactgctt gagcgcaagg taagatcgta ttgcgctcaa gcagtgagcc gccgcgcggc
      841 gaacgttagc gccctaagca agaacgctga gagttcttac tcccagcgcg acgctacgcc
      901 gccgccttcg tcccattcgc caccggcgtc gagggcgtcg tcgaaatcaa gcagtcggtc
      961 cagcaggaca ccggcgatca ggccgatggc ggcgacgccg accagagtgg cgaggctgcc
     1021 gatgccgatc ttttcggagc gtgtcaggcg gcggcgacct tcagtgtcga tgaccgtgga
     1081 tctgcccatc atgcgtcctc cagcgccagc gagcgcatgt atttcttgac cttggacatc
     1141 gcccgatcgc gcttcaggcg cgacagatgg tcaatgaaca gcacgccttc gaggtggtcc
     1201 agttcgtgct ggaaacagac ggcgtaaaga ccgtcgattt cctcctcgac gcgctgaccc
     1261 tcacggttca gataggcgac gcggatacgg gccgggcgtt cgactgtatc gaaatactcg
     1321 ggcagggaca gacagccttc ctcgtactgg aacagctcgt ccgaggacca caaaacttct
     1381 ggattgacga agaagcgcgg gttggagcga cggtcgtcct cgacctcggc gcccgcctca
     1441 tcgaccttct ggacatcgcc ttcgggcagg tcgccgtcgt gcaggtccat gaccacgacg
     1501 cggcgcaggt cgccgatctg gacggcggcc agaccgatgc ccggcgcggc gtacatcgtc
     1561 tccagcatgt cgtccatcag ggcgcgaacg tcatcgtcca cggcctccac gggcttggag
     1621 atctgcttca gcacagccag gtcggcagcg ttgtcgatgg tgagaatacg tcttacggtc
     1681 atgggcttca ggtaggcgcg aaggcccttc aggtcaagat ttcgggcctt ttcggcaggt
     1741 tattcagctg cagaagggtc ttccggcagg gctctgggct tccgatgctc gtcgatggcg
     1801 acgtaggtga agacgccctc ggtaacccgg ctgatatgat gcgaggcgcg cggacgcttc
     1861 catgcctcga cgtgaacgcg gatcgaggtg cggccgacac gcagcacacg agcatagatc
     1921 gagacctcgt ccccgaccga gaccggctgg tgaaagacca tggcgtcaac ggcgatggtg
     1981 gcgcagcggc cacgcgccag atcgaacgcc ggcgtggacc ccgcgaggtc catctgggcc
     2041 agcaaccagc cgccgaagat gtcgccctcg gggttggtgt cggcgggcat ggcgatgacg
     2101 cgcacgacgg gttgctggtc gggggcctgc agcggggtct ccgggggagt atcggacatc
     2161 ttatccttga gcattgcgaa tggcggccgc ccccgcgaag ggcgcgatga cggcggcgaa
     2221 cagggcgtag gcggccagaa gggccagagc ggggccgggc gacaaaccct gaacagcccg
     2281 agcgagcgcg cccgacccga acaccaccgg cggaatgaac aggggcagga cgaccacggc
     2341 gatcaacagg ccgccacgtt tggctccgag cgccaacgcc gcgcccagcg ctccggtgaa
     2401 ggcgaaaccg aggccgccca gacccgccgc cagcgccgtc aacggcgcga ggtggatcgg
     2461 ctggccgagc gcaagcgccg cgaccggcgc ggtcagggcc agcggcagac ccgtggcgat
     2521 ccactggctt aaggccttga tgacgaagac ggattccagc ggcagatggc cggtcgcgag
     2581 cagatcgagc gctccatcct cgagatcacg ctcgaacaac cgctccaacg acaggaggga
     2641 cgacagggcc agagctagcc aggcgacgcc cgccgccacg ggcccaagcg ttttgaggtc
     2701 accccccgcc gccagaggca ggattgcggt cagacaggcg aagaaggcgc aggccagcag
     2761 tggtccgccg ccccctcccc aggccagcga cagctcacgc tcgaacagca cgcgggtcgc
     2821 cccacgcagt ccgggagcgt ggggctccac cgcgccgggc tcgggcgtct ccggtttcgc
     2881 ctcgttccac tttgtaaatt tcggaccacc ggcgctcatg tgaggccgcc gagatcgagc
     2941 gcacgcgccg ggaaaggcag ggcgtcgtga acggcggcga tgatcagccc gtcggcacgg
     3001 agatgccgct gcatcgcctc ggcgaaggcc gaccgccagc gtgcgtccag cggcgccatg
     3061 ggctcgtcca acagccaaag cgcgcgcggt gacccgacca gccgccccag cgctagcctg
     3121 cgcctctgtc ccgccgacag tttgcggacc tccagatcca gcaatggctt cagcccgaac
     3181 gtctcgatcg catcatcgac accgtcctgg gtatgcccga gccagtcgca ctggaacgcc
     3241 agttcctcgc gcgccgtccg tgcgcccttt agtccatcca gatggccgag cagatgaacc
     3301 tcgcgacccc gtgcttcgcg cgcctcaagg acgttgtggt caccgtcgcg aaaggtgatc
     3361 tcgcccgctt ccgatcggat caatccggcg accgcgcgca acaggctggt cttgcccgcc
     3421 ccgttggcgc cggtcagtgc gacgacctcg ccagccttca gaacgaagtc cagatcgcga
     3481 aacagcacgc gctcgccccg cacgatcgac aatccttgca agctcaggga atggatcatc
     3541 ttttgcgagc cactctcaat gtcctcacag gcgacgcggg tacatggact gtacacgtta
     3601 ccagagctat agcctccggc gccgcagcag gctttcgccg cgcgcgccgg ggacctgtgt
     3661 gacccggcgt ctgtcgcgcg gaggcgcaac cggattgtcg ggcggcgtac accagaggaa
     3721 gactctccat gccgtcgatt gacagcctca agacccgcca ggatctctcc gtcgggcgca
     3781 agaaatacgc ctattacagc cttcccgccg ctgaggaagc cggtctgacc gggatttccc
     3841 gcctaccgcg ctcgatgaag gtgctcctcg agaacctgtt gcgcaacgaa gacggcgttt
     3901 cggtcaccga ggacgacctc aaggccgtcg ccgcctggat cgagaacaag ggctcggtcg
     3961 agcacgagat cgccttccgt ccggcccgcg tcctgatgca ggacttcacc ggcgttcccg
     4021 ccgtggtcga tctggccgcc atgcgcgacg ccatgaccgc gctcggcgcc gacgcctcca
     4081 agatcaaccc gctgaacccc gtcgatctgg tcatcgacca ctcggtcatg gtcgatcact
     4141 tcggcacctc gggcgccttc ggccagaacg tcgaacgcga atatgagcgc aatatcgagc
     4201 gctacaactt cctgcgttgg ggttcgtcgg cgttcaacaa cttccgtgtc gttcctcccg
     4261 gcaccggcat ctgccaccag gtcaacctcg agaacctggc ccagaccgtc tggaccctcg
     4321 acgaaggcaa gaagaccgtc gcctatcccg acaccgtcgt cggcaccgac tcgcacacca
     4381 ccatgatcaa cggcctggcc gttctgggct ggggcgtcgg cggcatcgag gctgaagcgg
     4441 ccatgctcgg tcagccgatc ccgatgctga tcccggaagt catcggcttc cgcctgtcgg
     4501 gcgccatgcc agagggcacg accgcgaccg atctggtgct gaccgtcacc cagatgctgc
     4561 gcaagaaggg cgtcgtcggc aagttcgtcg agttcttcgg cccggccttg ccgaacatga
     4621 cgatcgaaga ccaggcgacg atcgccaaca tggccccgga atacggcgcc acctgcggct
     4681 tcttccccgt ctccgccgcc accatcgcct atctgaccgc caccggccgc gacaaggcgc
     4741 gcgtcgccct cgtcgaagcc tacgccaagg ctcaaggcct gtggatcgat gaaacctcgg
     4801 aagacccggt cttcaccgac gttctggaac tggacatctc gaccgtcgtg ccgtcgctcg
     4861 ccggccccaa gcgtcctcag gacaaggtcg aactgaccat cgccgcgccg tcgttcgaga
     4921 ccgccctgac ggacatcttc aaccgtccgt cggatgcccc tcgctacgcc gtcgacggtg
     4981 agaagttcac ggtcggcgac ggcgacgtcg tcatcgcggc catcacctcc tgcaccaaca
     5041 cctccaaccc gtcggtgctg atcgccgccg gtctggtggc gcgcaaggcc cacaagctgg
     5101 gcatgaaggt caagccgtgg gtcaagacct cgctggctcc cggctcgcag gtggtcaccg
     5161 actatctgac cgacgccggt ctgcagaaag acctcgacgc gctgggcttc aacctcgtgg
     5221 gttacggctg caccacctgc atcggcaact cgggcccgct ggcgcctgcg atctccaagt
     5281 cgatcaacga caacgccatc gtcgccacct cggtcctgtc gggtaaccgc aacttcgaag
     5341 gtcgcgtgaa cccggacgtc caggccaact atctggcctc gccgccgctg gtcgtcgcct
     5401 atgcgctggc cggttcgatg cggatcgaca tcaccaagga tccgatcggt caggacaaga
     5461 agggcaacga cgtcttcctg aaggacatct ggccgacgac cgccgagatc gccgtcatcc
     5521 agaagaagtc ggtcaccccg gccatgttcg ccaaacgcta tgccgacgtc ttcaagggcg
     5581 acaagcattg gcaggccatc aaggtcaccg gcggccagaa ctacgcctgg gacgatagct
     5641 cgacctacgt gcaaaaccct ccgtacttcg agggcctgtc gatggaagcc aagccggtca
     5701 ccgacatcgt cgagggccgc gtgctggcca tcttcggcga ctcgatcact accgaccaca
     5761 tctccccggc cggttcgatc aagacctcgt cgcccgccgg cgcctatctg gtgaaccacg
     5821 gcgtcgatcc gctcgacttc aacggctacg gcgcgcgtcg cggcaaccac caggtcatga
     5881 tgcgcggcac gttcgccaac atccgcatcc gcaacaagat cacgtccgac atcgaaggcg
     5941 gcgtgaccaa gcatttcccg tcgggcgaca cgatgtcgat ctatgacgcg gccatgcgct
     6001 accagtcgga aggtcgtccg ctggtcgtgt tcgcgggcaa ggaatacggc accggctcat
     6061 cgcgtgactg ggccgccaag ggcaccaaac tgctgggtgt ccgcgccgtc atcgccgaaa
     6121 gctacgagcg tatccaccgt tcgaacctcg ttggaatggg cgtcgtgccg ctgcagttca
     6181 aggctgacgg ctggcagaag ctgggtctaa ccggtgagga aatcgtcacc gtgcgcggcc
     6241 tgtccgacgt caacatcggc aagctgcgtc cgcgtcagga cctgtgggtc gagctgttcc
     6301 gcccgtcgga cggcaagatg gcccgcttcc cggtccgctg ccgcatcgat aaccagaccg
     6361 agctggacta cttcaaggcc ggcggcgtca tgccctacgt cctccgtaac ctcgccgccg
     6421 gcgctgtcgc cccgacgccc gagccggaaa cggaagtcct cgccgccgag taagatcggc
     6481 ccgcgacggt tcgaaacaag aaaggccggt ggagcgatcc accggccttt ttccgtgttt
     6541 gcgatgtcgt ggcatctcag gacgtgaact cctaagtccg taatcctggg gcgggtgttc
     6601 accgaggtcg acaatgcttg gtggtagcga tctgaacgtc tggatccggc gccggtgctg
     6661 atggctcctc acgacggaag gctggaagct ggcaaatcat cggcgaacga aagctccgat
     6721 tcagccgagc atgacaaggc gggctattcg atcgctgccc gatcggcgcc caaacgttcg
     6781 gcgtagaggt ccaaggtgcg ttgccacgcc ggtcgagaca tggcgcagac gtaaaaggcc
     6841 ttcacattcg gatagccgtc catcaggttg gtttggcgaa gctggcgcag gacggcggcc
     6901 agcagcaggt ctgcgatggt gaaggcgtcg gtggctatcc agtcgcggcc ctcaagcctg
     6961 cggttcacgc cgtcgagcac gcggtgggtc cagtccatca ggaaggcgcg cgcatctccg
     7021 ttggcgctct tgggatcgcc catcgctccc atgttcatca gcttgagcgg ctggatcgga
     7081 ccctcgaccg tggccagggc ggcaagacac cattgaatga catgggcgcg gccctccgcg
     7141 tcggccggga tgaggcatcc cgatttctcc gccagatgga tcagaatggc ggcggattcg
     7201 gagatcgtca cgtcgccatc ggtcaagaca ggaatctggt tgaaggggct gatggccgag
     7261 aattccggcc cctttgtctc tccaccagtg tggtcccagc cgcgcacttt gtagggcagg
     7321 cccatctcct caagcgccca ctggatacgc agatcgcgcg tctccccatg gacgaaggga
     7381 tggacgcggc caaaagcgtg aagagtgatc atggaggcat cctcgcccat ccagtgagca
     7441 ggggaaagag gatgttcctc cgctgcagtc gccctcacgc catcggcggc ggacccgcga
     7501 gcacgtcgcg tttgccggag tggttggcct ggctgacgac gccttcctgc tccatccgct
     7561 cgatcagcga ggcggcgcgg ttgtagccga tctgcaggcg gcgctggacg taggaggtcg
     7621 aggccttgcg gtcgcgggtg accacggcca cggcgcggtc atacaggtcg tcgccggacg
     7681 aaccgccgcc gtcatcccca cccacagcgt tgtcgccgtc gccatcggga tcgtcggtga
     7741 tcaggtcgag gtagtcgggc tcggcctgtg agcgcaggtg ggcgcagacg gcctcgacct
     7801 cggcgtcggt gacgaacgga ccgtgcaggc gagtgatgcg gccgccaccg gccatgtaga
     7861 gcatgtcgcc ctgacccagc agctgttcgc cgccctgctc gccgaggatg gtgcggctgt
     7921 cgatcttcga cgtcacctgg aaggaaatac gcgtcgggaa gttggccttg atggtgccgg
     7981 tgatgacgtc gaccgacggg cgctgggtcg ccatgatcag gtggatgccg gcggcgcggg
     8041 ccatctgggc cagacgctgg acggcgccct cgacgtcctt gccggcgacc agcatcaggt
     8101 cggccatctc gtccatgacg acgaccagat agggcagggg ctcggggcgg atcttctcgc
     8161 tctcgtagac cgggcgacct tgatcgtcga agccggtctg gacggtacgc tcgaaatgct
     8221 cgcccttggc ctgggcctca cgggcgcggt cgttgtagct ggcgacgttg cggacgccga
     8281 gcttggacat ccggcgatag cggtcctcca tctcgcgcac gacccatttc agggcgacga
     8341 cagccttctt cggatcggta acaacgggcg ccagcaggtg cgggatgccg tcatagaccg
     8401 acaactccag catcttgggg tcgatcatga tgaagcggca ttcggacggc gacaggcggt
     8461 acaggatcga caggatcatg gcgttgaccc cgaccgactt gccggaaccg gtggtgcccg
     8521 cgatcagcag gtgaggcatg cgtgccagat cggcgacata gggctcgccg ccgatgcttt
     8581 cgcccagcgc cagcggcagg atgtgcgcag gcttggcgta ttcggccgag gccagcaggt
     8641 cgcgcagata gacagtctcg cgcttggcgt tgggca
//
LOCUS       DASVTM010000221         5327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_21669, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000221 DASVTM010000000
VERSION     DASVTM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21669"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF633_05950"
     CDS             <1..442
                     /locus_tag="VF633_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX8470636.1"
                     /translation="GTTISESVAICRYLEALYPEPNLFGVDAREQAVVEMWTRRNEFY
                     LANPIMLNVRHTHPAMAALEGVQMTEVADYNRVGAERFMRTLDRHLAEHEFIALNRFT
                     IADIVAVVGLDFARLVKYRPPEELTNLARWLEDCRTRPAAKAGA"
     gene            496..798
                     /locus_tag="VF633_05955"
     CDS             496..798
                     /locus_tag="VF633_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX8470637.1"
                     /translation="MAVTANIHEAKTTLSKLIERAIAGEEVIIAKAGKPLVRLAPVEK
                     PVAVDRAAIFGSMRDKMNLQDGWEDSAFDAEMERKWFGDAATTLPHAAEPGRPFKR"
     gene            798..1172
                     /locus_tag="VF633_05960"
     CDS             798..1172
                     /locus_tag="VF633_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8470638.1"
                     /translation="MRLLLDANIVIWLLGSPERISATALQALLNPDNRLFVSTASLME
                     IVAKAATGRLTFDEESRRRTELFADWMIVTAEHAWRIGSLPPIHKDPFDRIIVAQALV
                     EGMTLVTGDRLLLEYGVSILLA"
     gene            complement(1173..1607)
                     /locus_tag="VF633_05965"
     CDS             complement(1173..1607)
                     /locus_tag="VF633_05965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8470639.1"
                     /translation="MVAKITVSEGEFAGWKTYDLHGTFDQTVGPFYFKPDPDGRMRCA
                     FRAEQKHMNAGHRMHGGCLMTFADIALFQTAYQEMEGKNGVTVQLDSTFIDGALVGEL
                     IEATGEVVRAGGSLIFVRGQITTGERTLMTFSGVIKKFTPRG"
     gene            1691..2014
                     /locus_tag="VF633_05970"
     CDS             1691..2014
                     /locus_tag="VF633_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HEX8470640.1"
                     /translation="MSDPIETAIFEKLSRVDPKGDNGKSIEPAEVAKELQPEQWQRML
                     PKVRGVALGLMRQGKLTITKKGKPIDPASFRGVIRLRLPTADETAAALAANPPVVNED
                     DDDFA"
     gene            1998..2636
                     /locus_tag="VF633_05975"
     CDS             1998..2636
                     /locus_tag="VF633_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX8470641.1"
                     /translation="MTTSPDNFGPEHFGSDPDTDLEDVLADIRACRACLGELPHTPRP
                     VVRVFPETRLLICGQAPGRRVHESGLPFTDPSGDRLRQWLGVDYDTFYADHRIGVAAQ
                     AFCYPGTALKGGDYPPPRRCAELWRPRLIDALPQMELTLLVGGYAQVWALGDRVGSNM
                     TETVRNWRAYAPQFLPMPHPSWRNTAWLRKNPWFEEEVVPYLRQRVSDILTS"
     gene            2633..3676
                     /locus_tag="VF633_05980"
     CDS             2633..3676
                     /locus_tag="VF633_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8470642.1"
                     /translation="MTIRIAGLFAAALALASCTTPLVQSPLTPPAGFAGPRIKDAASP
                     GMTGAFIVQDGARLPFLHWAPADGKPWATIVALHGMNDHDASFRLAGPWWAEHGIEVW
                     SFDQRGFGQAPAEGIWAGEALLTEDLRTVTALVRARRPDATLAVVGESMGGSVAAAAF
                     GGPNPPDADRVVLLSPGVWGWSSQNILNRTSLWIASHTVGDLSVEPPDFIARHILASD
                     NIVELIRNGRDPDTLLSTRFDAISGLVDLMESASNRIGLIKAPVILMYGAHDQVVEPG
                     PMRRALERAGTPPSLRTAFYDDGWHLLNRNLGAERMYRDVEAYLRDASAPLPSGVPPV
                     LPELQKGRLTKVR"
     gene            3765..>5327
                     /gene="fliF"
                     /locus_tag="VF633_05985"
     CDS             3765..>5327
                     /gene="fliF"
                     /locus_tag="VF633_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX8470643.1"
                     /translation="MGGFTAALQRFGVGRLAAVLGVAAGVAAVLVALMLRVGQAPDAL
                     LYSNLDLKEAGDITASLEQAGIKYKVQGDGSTIMVNRDDVGTARMMVNSKGLVTTGSV
                     GYEIFDNQSVLGQTEFQQNLNEKRALEGELGRTILSYRGISSAKVLIALPRREMFASS
                     TGEPTAAVVVGVGGRTLTGDQIQAIRNVVASSVPGLKPEKVTVTDTANRTLAAGSGED
                     GFSAASAQDAKDTTEAQLQARIMDIVEGVVGAGAARVQVTADIDHSRSTTQEQKYDPD
                     GQVVRSTSTNGSEKTDTTGDPTGGTTATNNIPGGQAPTTTPAGSSDKANTETTNYEIS
                     NITTTTVKEAGQIQKLAVAVNVDGIWTPAADGKGEPTYAPRSAEDIAKIDALVKAAIG
                     FDQQRGDQVKVDNVKFSRDTSAAGGTETGSSLLDFDKNDIMRGVELLVLLVTGLLLIF
                     FVLRPLLKTANTTGVPAIASGGGGGGLPVTSLQTVMVDGVPQLLAAPASDMDQRLDIA
                     RIEGQVKASSIKK"
BASE COUNT          952 a         1707 c         1705 g          963 t
ORIGIN      
        1 cggcacgacg atctcggagt cggtggccat ctgccgctat ctcgaagcgc tctacccgga
       61 gccgaacctg ttcggcgtgg atgcgcgtga acaggcggtg gtcgagatgt ggacgcgccg
      121 gaacgagttc tatctcgcca atccgatcat gctgaatgtg cggcacaccc atccggccat
      181 ggcggcgctg gaaggcgtgc agatgaccga ggtcgccgac tacaaccggg tcggagccga
      241 gcggttcatg cggacgctgg atcggcatct ggcggaacac gagttcatcg ccctgaaccg
      301 gttcaccatc gccgacatcg tcgccgtggt cgggctcgat tttgcccggt tggtcaaata
      361 ccggccgccc gaggagctca ccaatctggc ccgctggctt gaagactgcc gaacaaggcc
      421 tgcagctaag gctggcgcat agataacgtc ggtacccagc ttgcgccggt tggttagcta
      481 gcctagctta gttgcatggc tgtcaccgca aacattcacg aagccaagac cacgctgtcg
      541 aagctgatcg aacgcgccat cgcgggcgaa gaggtcatca tcgccaaggc cggcaagccg
      601 ttggtgcgac tggctccggt agagaagccc gtcgcggtcg atcgggcggc gatcttcggc
      661 agcatgcggg acaagatgaa cctgcaggac ggttgggaag actctgcgtt cgacgccgag
      721 atggagcgca aatggttcgg cgatgcggcg acaaccctcc ctcatgccgc tgagcctggc
      781 aggcctttca agcgctgatg cgactgctgc tcgacgccaa tatcgtgatc tggctgctcg
      841 ggagtcctga acggatatcg gccaccgcat tgcaggcgct tctgaacccc gacaatcgat
      901 tgttcgtcag cacggcgtcg ctgatggaga ttgttgcaaa ggcggcgacg ggtcgcctga
      961 ccttcgacga ggagtcgcgc cgccggacag aactgttcgc ggactggatg attgttacgg
     1021 cggagcatgc gtggcggatc ggaagcctgc cgccgatcca caaggatcct tttgaccgca
     1081 tcatcgtcgc gcaggcgctg gttgagggca tgaccctcgt caccggcgat cgtctgctgc
     1141 tggaatacgg cgtatctatt cttcttgcct gatcagcccc tcggcgtgaa cttcttgatc
     1201 acgccggaga aggtcatcag cgtgcgctcg ccggtggtga tctggccgcg gacgaagatc
     1261 agcgagccgc ccgcgcgcac gacttcgcca gtggcctcga tcagttcgcc gacgagggcg
     1321 ccgtcgatga aggtcgagtc cagttggact gtgacgccgt tcttgccctc catctcctga
     1381 taggcggtct ggaacagggc gatgtcggcg aaggtcatca ggcaaccgcc atgcatgcgg
     1441 tgcccggcgt tcatatgctt ctgctcggcc cggaaagcac aacgcatccg gccgtcggga
     1501 tcgggcttga aatagaaggg accaaccgtc tggtcgaagg tgccgtgcag gtcgtaggtc
     1561 ttccaacccg cgaactcgcc ttccgatacg gtgattttcg cgaccatcgc cttttccttg
     1621 cctaccgcgc ttgcgccact tcaggcgatt ccgccttctt tctgatctcg caatgcagca
     1681 tataggcgca atgagcgacc ccatcgaaac cgcaatcttc gaaaaactct cccgcgtcga
     1741 tcccaagggc gacaacggca agagcatcga gcccgccgaa gtggccaagg aactgcagcc
     1801 cgagcagtgg caacgcatgc tgcccaaggt tcgcggcgtg gccctcggcc tgatgcgtca
     1861 aggcaagttg accatcacaa agaagggcaa gccgatcgat ccggcgtcgt tccgtggcgt
     1921 gatccgtctg cgccttccga cggcagatga gaccgctgcc gcgctggctg cgaacccccc
     1981 ggtcgtcaac gaggatgatg acgacttcgc ctgataactt tgggcctgag catttcgggt
     2041 ccgatccgga tacggatctc gaagatgtcc tcgccgacat tcgggcgtgc cgcgcctgtc
     2101 ttggtgaact gccgcatacc ccgcgccccg tagtccgcgt ctttcccgag acgcggctgc
     2161 tgatctgcgg ccaggcgccg gggcgacggg tgcatgagag cggcctgccg tttaccgatc
     2221 cgtccggcga cagactgcgc cagtggctgg gcgtcgacta cgacaccttc tacgccgacc
     2281 atcgcatcgg ggtggcggcg caggccttct gctatcctgg gacagccttg aagggtggtg
     2341 actatccgcc gccgcgccgg tgcgcagaac tctggcggcc gcggttgatc gatgccctgc
     2401 cgcagatgga actgaccctg ctggtcgggg gctatgccca ggtctgggcg ttgggtgatc
     2461 gagtcggatc caacatgacc gaaaccgtgc ggaactggag agcgtatgcg cctcagttcc
     2521 tgccaatgcc gcatccgtcc tggcgaaaca cggcctggct gagaaaaaat ccctggttcg
     2581 aggaggaagt cgtgccttac ttgcgccagc gtgtctcgga catcctgacc tcatgaccat
     2641 tcgtatcgca ggcctgttcg cagcagcgct cgcgctggcg tcctgcacca cgccattggt
     2701 ccagtcgccg ctgacgccgc ctgcagggtt cgccggaccc cggatcaagg acgcggcgtc
     2761 ccccggcatg accggagcct tcatcgttca ggacggagcg cgattgccgt tcctgcactg
     2821 ggcccctgcc gacgggaagc cctgggcaac gatcgtggcg ctccatggca tgaacgatca
     2881 cgacgcctcg ttccgcctcg caggtccatg gtgggccgag catggcatcg aggtgtggag
     2941 cttcgatcag cgcggctttg gtcaggctcc cgccgagggg atctgggccg gagaagccct
     3001 gctgaccgaa gacctgcgga ccgtcacggc actggtccgc gcccgtcgtc ctgacgcgac
     3061 gcttgctgtg gtcggagaga gcatgggcgg ctcggttgcg gcagcggcgt tcggaggtcc
     3121 caatccgccg gacgccgatc gcgtggtgct gctgtctcct ggcgtctggg gctggtccag
     3181 ccagaatatt cttaaccgca ccagtctgtg gatcgcctct cataccgtgg gcgacctgtc
     3241 ggtggagccg cccgacttca tcgcccgcca cattctggcg tccgacaata tagtggagct
     3301 gatccgcaac gggcgcgatc ccgacaccct gctatcgacc cggttcgacg ccatctccgg
     3361 tctggtcgat ctgatggaga gcgcgtccaa ccggatcggc ctgatcaagg cgccggtgat
     3421 cctgatgtac ggggctcacg atcaggtggt ggagcccggg ccaatgcgtc gggcgctgga
     3481 gcgggcaggc acgccaccca gcctgcgcac cgccttctat gacgatggct ggcatcttct
     3541 gaaccgcaac ctcggggccg agcgcatgta tcgtgacgtc gaggcttatc tgcgcgacgc
     3601 ctccgcgccc ttgccgtcag gtgtgcctcc tgtgttgcct gagctgcaaa aagggcggct
     3661 aaccaaagtt cggtaacgtc tcttaacgcg acatttacca tgcgggcggc agtttctgcc
     3721 tagcgggtgt gtctgttccc ccgtcagtaa tcgggtgggg acgcgtgggc ggctttacgg
     3781 cggcgttgca aagatttggg gtcggccgtc tggctgcggt tctcggcgtc gccgccggcg
     3841 tcgccgccgt gctcgtggcg ctgatgctgc gcgtcggcca ggcgcctgat gcgctgctct
     3901 attccaacct cgacctgaaa gaggcgggcg acatcaccgc ctcgctcgaa caggcaggca
     3961 tcaagtacaa ggtccagggc gacggctcga ccatcatggt caatcgcgac gacgtcggca
     4021 ctgcgcggat gatggtcaac tccaaggggc tcgtgaccac gggttccgtc ggctacgaaa
     4081 tcttcgacaa ccagtctgtt ctgggacaga ccgagttcca gcagaacctc aacgaaaagc
     4141 gcgctcttga aggcgagttg gggcgcacca tcctgtcata ccggggcata tcctcggcca
     4201 aggtgctgat cgccctgccg cgccgcgaga tgtttgcttc atctaccggc gagcccaccg
     4261 ctgccgtggt ggtcggcgtc ggcggccgga ccctgaccgg cgaccagatc caggcgatcc
     4321 gcaacgtcgt cgcttcgtcc gtgccgggcc tgaaaccaga gaaggtcacc gttaccgaca
     4381 ccgcaaaccg caccctggct gcgggctccg gcgaggacgg tttctcggcg gcctccgccc
     4441 aggacgccaa ggacaccact gaggcccagt tgcaggcccg tatcatggac atcgtcgagg
     4501 gcgtcgtcgg cgcaggcgcg gcgcgggttc aggtcacggc tgacatcgac cacagccgct
     4561 cgaccaccca ggaacagaaa tacgatccgg atggccaggt cgtccgctcg acctcgacca
     4621 acggctcgga aaagaccgac accaccggcg atcccaccgg tggcacgacg gcgacgaaca
     4681 acatccccgg tggtcaggct ccgacgacga cacctgccgg ttcgtccgac aaggccaata
     4741 ccgagaccac caactacgag atatccaaca tcacgacgac cacggtcaag gaagccggtc
     4801 agatccagaa gctggccgtt gcggtcaatg tcgacggtat ctggacgccg gctgcggacg
     4861 gcaagggcga accgacctat gcgccgcgct cggccgaaga tatcgccaag atcgacgccc
     4921 tggtgaaggc cgccatcggc ttcgaccagc agcgcggcga ccaggtgaag gtcgataacg
     4981 tcaagttcag ccgcgacact tcggcggccg gaggcacgga aactggctcc tctctgctgg
     5041 atttcgacaa gaacgacatc atgcgcggcg tcgagctgct ggtgctgctg gtgaccggcc
     5101 tgttgctgat cttcttcgta ctgcgcccgc tgctgaagac cgccaacacc acgggggttc
     5161 cggccatcgc cagcggcggc ggcggtggcg gcctgcccgt gaccagcctg cagacggtca
     5221 tggttgacgg tgtgccgcag ttgctggcgg cccccgcgtc ggacatggat cagcgcctcg
     5281 acatcgcccg gatcgagggt caggtgaagg cctcctcgat caagaag
//
LOCUS       DASVTM010000222         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_234919,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000222 DASVTM010000000
VERSION     DASVTM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234919"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF633_05990"
     CDS             <1..258
                     /locus_tag="VF633_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEX8470644.1"
                     /translation="GESGVAYRLGGPSCLAGDIIGDYVFTTEPTVGTRIAFLDQAHYS
                     MVKTNTFNGVPLPAIALWNSETDTLKLVREFDYSEFRDRLS"
     gene            complement(255..2861)
                     /locus_tag="VF633_05995"
     CDS             complement(255..2861)
                     /locus_tag="VF633_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX8470645.1"
                     /translation="MILRRLLTAILATVAFASQAPAKAASEPTAEARAAELVSRMTLA
                     EKVSQIQHDAPAIPRMAIPAYNWWSEGLHGVARADVATVFPQAIGMAATWDTELIHTV
                     ADTIATEFRAKYVAGLAADGSSRQYRGLTVWSPNVNIFRDPRWGRGQETWGEDPYLTS
                     RFGVAFIEGLQGDDPLHPKTIAAVKHFAVHSGPEAGRHHDDIHPSPRDVTETYLPAFH
                     AAVTEAQAQALMCAYNAVDGVPACANTDYLKDRLRTDWRFDGHVVSDCAAVADIYLPT
                     SHATVRTPEEAVALALKAGTDLICDFKFNATTDPATTVRAVEQGLLSEAELDTAVTRL
                     FVARIRLGTLDPASAGAFADIAATDFDTPEHRALALETARKSLVLLKNDGLLPLKAAP
                     RRIAVIGPNADSLDALTGNYNGTPSSPVTVLAGLKVRFPDSEINFVEGTGWVAPPLED
                     VPDAAFCRDRDCSLPGLRAEEFSGPELDGTSVVRDDVNVVFGWGRPDRAERHTSIRWT
                     GFLKVTEPGEYRFRYTGDSGYRVFVDDRPIADVWDAAWPTSDTAVTLEAGRTYAIRVE
                     ASQKGARATQRLRWSRPGVGDEPALAAARNADLIVFVGGLTARLEGEEMSVQAPGFAG
                     GDRTSLDLPAPQEQLLERLHATGKPVVLVLMNGAAMSVNWADANLPAIIEAWYPGGDG
                     GRAIAELIAGDFSPSGRLPVTFYKTADDLPPFTDYGMDGRTYRRFTGTPLYTFGHGLS
                     YTRFGYGEIQSDKTEVSAGSSVRVSVDVTNSGDRDGDEVVQLYVSRSGEAAPIRSLAG
                     FQRISLKAGETRRVSFDVSSGALGVVDAAGHRQVTAGRASIWIGGGQPGGSAVGAATT
                     LTITGSREVAAF"
BASE COUNT          480 a          956 c          978 g          482 t
ORIGIN      
        1 ggcgagagcg gcgtcgctta ccggttgggc ggcccatctt gtctggctgg cgacatcatc
       61 ggcgactacg tcttcacgac tgagccgacc gtcggtacgc gcatcgcctt ccttgatcag
      121 gcccactatt cgatggtcaa gaccaacacc ttcaacggcg tccccctccc cgccatcgcc
      181 ttgtggaaca gcgaaacaga cacgctgaag ctggtccgcg aattcgatta ctccgagttc
      241 cgcgaccgcc tgtcctagaa ggctgcgact tcacgcgagc cggtgatcgt cagggtcgtg
      301 gctgcgccga ccgctgagcc tccgggctgg ccgccgccga tccagattga ggcccggcct
      361 gccgtgacct gacgatggcc cgccgcatcg acgacgccca atgcgccgga tgaaacgtcg
      421 aaggacacgc ggcgagtctc tccggccttc aacgatatgc gctggaagcc cgcgagactg
      481 cggatcggcg cggcctcgcc tgaacgcgag acatagagct gcaccacctc gtccccgtca
      541 cggtcgccgc tgttggtgac gtcaacggaa acgcggacgc tagatcctgc ggagacttcg
      601 gtcttgtccg actggatttc gccatagccg aagcgcgtgt agctgagccc gtggccgaac
      661 gtgtacagcg gcgtaccggt gaagcgccgg taggtgcggc cgtccatgcc gtaatcggtg
      721 aacggcggca ggtcgtcggc ggtcttgtag aaggtcaccg gtagccggcc cgaaggactg
      781 aagtcgcccg caatcagttc ggcaatcgcg cgaccgccgt cgccgcccgg ataccaagcc
      841 tcgatgatgg cgggcagatt tgcgtcggcc cagttcacgc tcatcgccgc gccgttcatc
      901 agcaccagca cgacgggttt gccggtggcg tgcaggcgtt cgagcagttg ctcctgcggc
      961 gcgggcagat caaggctggt gcggtcgcca cccgcgaagc ccggagcctg aaccgacatc
     1021 tcctcgccct cgagtcgcgc ggtcagtcct ccgacgaaaa cgatgagatc ggcattgcgg
     1081 gcggcggcca aggcgggctc gtcgccgacg ccgggacggc tccagcgcag acgctgcgtt
     1141 gcgcgcgcgc ccttctgaga ggcctcgaca cggatcgcat aggtccgacc ggcctccagc
     1201 gtcacggcgg tgtccgaggt cggccatgcg gcgtcccaga cgtctgcgat cgggcgatca
     1261 tcgacgaaga cgcggtagcc gctgtcgcct gtgtagcgga accggtactc gcccggctcc
     1321 gtcaccttga ggaaaccggt ccagcggatc gaggtgtgac gttccgcccg gtcgggtcgt
     1381 ccccagccga acacaacatt gacgtcgtcg cggacgacgg atgtaccgtc caactccggg
     1441 cctgagaact cctcggcgcg aagaccgggc aggctacagt cgcggtcgcg gcagaaggcg
     1501 gcgtcgggca cgtcttccag cggaggcgcg acccacccgg tgccttcaac gaaattgatc
     1561 tcgctgtccg ggaaacgcac cttcagcccg gccagtacgg tgacgggcga cgacggcgtg
     1621 ccgttgtagt tgccagtcag ggcgtcgagg ctgtcggcgt tcggcccgat cacggcgatg
     1681 cgacgtggtg cggccttcag cggcaacaga ccgtcgttct tgagcaggac cagagacttg
     1741 cgcgccgttt ccaacgccag cgcgcgatgc tccggcgtgt cgaagtctgt tgccgcgata
     1801 tcggcgaagg cgcccgctga tgccgggtcc agcgtgccca aacggatgcg cgccacgaac
     1861 agtcgcgtga ccgccgtgtc cagttcagcc tcggagagca gcccctgctc gaccgcccgc
     1921 acggtcgtcg ccggatccgt ggtggcgttg aacttgaagt cacagatcag atcggtcccg
     1981 gccttgaggg ccagcgcgac cgcctcctcg ggagtgcgga cggtcgcatg cgaggtcggc
     2041 agatagatgt cggcgacggc ggcgcagtcc gaaaccacat ggccgtcgaa cctccaatcc
     2101 gtccgtagcc ggtctttgag atagtcggtg ttagcgcagg ccggcacgcc gtcgacggcg
     2161 ttataggcgc acatcaaggc ctgggcttgg gcctcggtca ccgccgcgtg gaaggcggga
     2221 agataggtct ccgtcacgtc gcggggcgag ggatggatgt cgtcgtggtg ccgcccggct
     2281 tcggggccgc tgtggactgc gaaatgcttc accgccgcga tagtcttggg atgtagcgga
     2341 tcgtcgccct gcagaccctc gatgaaggcc acgccgaagc gcgaggtcag ataagggtcc
     2401 tcgccccagg tttcctgacc gcggccccag cggggatcgc ggaagatgtt cacgttgggc
     2461 gaccacacgg tcagcccccg gtattgacgc gaactgccgt cagctgcgag gcccgcgacg
     2521 tatttggccc ggaactcggt ggcgatcgtg tcggcgaccg tgtggatcag ctctgtatcc
     2581 caggtggcgg ccatgccgat ggcttgagga aacaccgtcg ccacgtcggc ccgggcgacg
     2641 ccgtgcagac cctcgctcca ccagttgtag gcggggatcg ccatcctcgg gatcgcgggc
     2701 gcgtcatgct ggatctgcga caccttctcc gccagcgtca tgcgagagac cagttcggcg
     2761 gctcgagcct cggcggtcgg ctcgctggcc gccttcgcag gggcttggct ggcgaaggca
     2821 acggtggcga ggatggctgt cagcaggcgt cgcaagatca tccggcatct ccgggcgcaa
     2881 cctccacgcc aacgat
//
LOCUS       DASVTM010000223         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_181671,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000223 DASVTM010000000
VERSION     DASVTM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181671"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VF633_06000"
     CDS             <1..624
                     /locus_tag="VF633_06000"
                     /inference="COORDINATES: protein motif:HMM:NF018389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEX8470646.1"
                     /translation="LDTMTALLRTGAKPASSVPPSPVLPVITAAPAVSQTGFNTPLDP
                     LNADETTRAAVPPPSLEALPAVKLCRENTAFNEGVQKAVTASRLNAATELWGIPCDSG
                     AYNINYAFYMTSPQGTDPRVVNFPGVDGVQIPTAEDGDHGWLTNPSYDPTTRTLIAFA
                     KGRGLGDCGVAQSWTWTGQAFVLNREQVMSDCFGMTSELWPTRFRSR"
     gene            621..1541
                     /locus_tag="VF633_06005"
     CDS             621..1541
                     /locus_tag="VF633_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8470647.1"
                     /translation="MMTDTDETPEVLIIGAGPAGLTAATYLARFLRKVVVADAGEPRA
                     CWIPVSHNMPGFPAGITGGAILSRMREQAEEYGAVVEAGTVEDLRAVDGGFVARLNGR
                     NITVRAVLLATGVVDHHPDLPGVEDAVRRSLVRICPICDGFEATDKAVAVIGDGDAGV
                     REAAFIRTYSDRVTLIHIGHANALTEREALQRLGISLVETAIEQVHLENDRVTAFSFG
                     GRRTAFDMVYSALGTSPNASLAERLGAERSDEDGRLVVDAHQQTSIPGFYAAGDVVHG
                     LNQIAVATAEAAVAATAIHNSLRQADGQTV"
     gene            1902..2822
                     /locus_tag="VF633_06010"
     CDS             1902..2822
                     /locus_tag="VF633_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8470648.1"
                     /translation="MSDPLSGIPLASIRVFEAAARLKSFTRAAEELGMTQAAVSWQIK
                     ALETRLGLSLFRRLPREVMPTEPGERLSRAATEAMLLLRRALADLTDSDQGVLSITTL
                     ATLASQWLAQRLGEFQIANPGLAVRVDTSSTLMDLTRENVDIAIRSGTGVWPGLEAAL
                     LMPSVFTPLCTPALCARLNLQTPSDLIAAPRIGEEKEWAAWFVEAGVEGVEHPAVRLR
                     ADFQALEVAAALGGQGVALASPILYSREIAAGMLVQPFPEMVELSPGYWICWPADRRR
                     SPKIVRFREWLLKAAADDPAITAALKTVGR"
     gene            complement(2819..>3813)
                     /locus_tag="VF633_06015"
     CDS             complement(2819..>3813)
                     /locus_tag="VF633_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8470649.1"
                     /translation="PGAEGYYDFGIRAYTTTTLTGDEVHALGLAQVAEISSRADAILK
                     AEGRTEGTVGVRLAEMAKDPRFIYPNTDAGKQTLLDELNAQVRAMQVRLPEYFGILPK
                     ASVEIRRVPPAIEAGAPGGYYQGPSLDGSRPGAYYINLRDTAEWPKWTLPTLTYHEAT
                     PGHHLQITISTEAQGIPTIRKLIGFSAYSEGWALYTEQLADEMGVYDQDAWGRLGFLQ
                     SQLFRATRLVVDSGLHHKKWSREQAIRYMVDTLGDQESSVTTEVERYCVWPGQASSYK
                     VGHTEWVRLREDAKAQLGPKFDIKGFHDVALSSGGMPLDVLKSVVGEWVTAQKA"
BASE COUNT          634 a         1227 c         1279 g          673 t
ORIGIN      
        1 ctcgacacaa tgacggccct gctgcggacg ggcgcgaaac ccgcgtcgtc cgtgcccccc
       61 tcgcccgtcc tgcccgtcat caccgccgcg cccgccgtca gccaaaccgg cttcaacacg
      121 ccgctggacc cgctgaacgc cgacgaaacc acgcgagccg ctgtgccgcc accatcgctc
      181 gaggccttgc ctgccgtgaa gctgtgccgc gaaaacacgg ccttcaacga gggtgtgcag
      241 aaggcggtca ccgcgtcgcg cctgaacgcc gcgaccgaac tgtggggcat tccctgcgac
      301 agcggcgcct acaacatcaa ctacgcattc tacatgacca gcccacaggg gactgatccg
      361 cgcgtcgtca acttccccgg agtcgacggc gtgcagatcc cgaccgcaga agacggggac
      421 cacggttggc tgaccaatcc atcctatgat cccaccaccc gcaccttgat cgctttcgcc
      481 aaggggcgcg gtctgggtga ttgtggcgtg gcccagagtt ggacctggac tggacaggct
      541 ttcgtgctga accgcgagca ggtgatgtcg gactgcttcg ggatgacctc agaactctgg
      601 ccgacgagat tccgcagccg atgatgaccg ataccgacga aactcccgaa gtcctgatca
      661 tcggcgccgg ccccgcaggg ttgaccgctg caacctatct cgcccgcttt ttgcgcaagg
      721 tcgtggtcgc cgacgccggc gaacctcgcg cctgctggat accggtaagc cacaacatgc
      781 ctggtttccc ggcggggatc acgggcgggg ccatcctgtc gaggatgcgc gaacaggctg
      841 aggaatacgg tgcagtggtc gaggccggga cggtcgagga cctgcgagcc gtcgacggag
      901 gcttcgtcgc gcggctgaat ggacggaata tcacggtccg ggccgtgctg ttggccaccg
      961 gcgtggtcga ccatcatccg gacctgcccg gcgtggagga cgcggtacgc cggtctctgg
     1021 tccgcatctg tccgatctgc gacggctttg aagcgacaga caaggccgtc gcggtgatcg
     1081 gggatggcga cgccggcgtg cgcgaagcgg ccttcatccg tacctattcg gaccgggtga
     1141 ctctgatcca catcggccat gcgaacgccc tgaccgaacg ggaggcgttg cagcgtctcg
     1201 gcatatcgct ggtcgagacg gctatcgaac aggtgcatct tgagaatgac cgcgtaaccg
     1261 ccttcagttt cggcggacgg cgcacggcct tcgacatggt ctattcagcg ttgggaacct
     1321 caccgaacgc ctcgttagcc gaaaggctgg gtgcggaaag gtcggacgag gacggccgtc
     1381 tggtcgtcga tgcgcatcag caaaccagca tcccgggctt ctacgccgcc ggtgacgtcg
     1441 tccacgggtt gaaccagatc gcggtggcga cggccgaagc ggcggtcgcg gcgacagcga
     1501 tccacaattc gctgcgtcag gccgacggcc agacggtcta ggcgagctcg atgcaggcct
     1561 cgttccggtc gggagagcgc tttggcgcgc acttcggtgg agcgtctgga aggaacctcc
     1621 gggctccacg gcatcactac tgaggcgtag cttctctctg gagcgtaacg gcacggggga
     1681 aggtcggtgc tgctgctcgc gcctcacggc ggagacgatc acgccgtccc tgcgcgatca
     1741 tgtcggccac gacggcgacc caaccccata caggttcctt atgcgtgcgg tgcatctctt
     1801 ccataacagc ctcctgcgct gtgattgggg aaaggggcaa cgtgggcttg acggtaggca
     1861 cgcacaaacc agatgatggt tgagagcaac aaggtctgtt tatgtctgat cctctctccg
     1921 gcattcccct cgcctccatc cgggtgttcg aggccgcagc gcgtctgaag agcttcacga
     1981 gggcggccga ggaactgggt atgacccagg cggcggtgag ctggcagatc aaggcgttgg
     2041 agacgcggct gggtctcagc ctgttccggc ggctgccccg cgaggtgatg ccaacggagc
     2101 ccggtgagcg gctgtcgcgt gcggcaactg aagcgatgct gttgttgcgc cgtgctctgg
     2161 ctgacctgac ggattccgat caaggcgtgt tgtcgatcac aacgctggcg acactagcca
     2221 gccaatggct ggcgcagcgg ctgggggagt tccagatcgc caatccgggc ctggcggtgc
     2281 gagtggacac atccagcacc ctgatggatc tgacgcgcga gaacgtcgac atcgccatcc
     2341 gctcggggac cggcgtctgg cccgggctgg aggcggcgtt gctgatgccc agcgtcttca
     2401 ccccgctctg cacgcccgcc ttgtgcgcca ggctgaacct gcagacgccg agcgatctga
     2461 tcgcggcgcc gcgcatcggc gaggaaaagg aatgggcggc gtggttcgtg gaggcgggcg
     2521 tcgagggcgt cgagcatcca gccgtacggc tccgggcaga ctttcaggcg cttgaggtgg
     2581 cggcggctct gggcggtcag ggcgtggccc tcgcgtcgcc tattctgtac tcgcgtgaga
     2641 tcgcggcggg aatgctcgtg cagcccttcc cggagatggt cgagctgagc cccggctact
     2701 ggatttgctg gcctgccgac cgtcggcgct cacccaagat cgtgcgcttt cgggaatggc
     2761 tgctgaaggc ggcggctgac gatcccgcca tcaccgccgc cctcaagacc gttggccgct
     2821 aggctttctg agccgtcacc cattcgccga cgaccgactt cagcacgtct agcggcatgc
     2881 cgcccgacga cagcgcgacg tcgtggaagc ccttgatatc gaacttgggg ccgagctgag
     2941 ccttggcgtc ctcgcgcagg cggacccatt ccgtgtggcc gaccttgtag ctggacgcct
     3001 gacccggcca gacgcagtag cgttcgacct cggtggtgac gctgctctcc tgatcgccga
     3061 gggtgtcgac catgtagcgg atcgcttgct cgcggctcca cttcttgtgg tgcaggccgg
     3121 agtcgacgac cagacgcgtg gcccggaaca gctggctctg caggaagccc agacggcccc
     3181 aggcgtcctg atcgtataca cccatctcgt cggccagttg ctcggtgtag agcgcccagc
     3241 cttcggagta ggcggagaag ccgatcagct tgcggatggt cgggatgccc tgggcctcgg
     3301 tcgaaatcgt gatctgcaga tggtggcccg gcgtcgcctc atgataggtc agcgtcggca
     3361 gggtccactt tggccattcc gccgtatcgc gcaggttgat gtagtaggcg cccggtcgcg
     3421 aaccatccag cgacgggccc tgataatagc ctcccggtgc accggcctcg attgccggcg
     3481 ggacacgccg gatctcgacc gaagccttgg gcaggatgcc gaaatactcg ggcagccgaa
     3541 cctgcatggc ccgcacctgc gcattgagct cgtccagcag ggtctgtttg cccgcgtcgg
     3601 tgttcggata gatgaagcgc gggtccttgg ccatttccgc cagacggacg ccgacagtgc
     3661 cctcggtcct gccctcggct ttcaggatgg cgtcggcgcg actgctgatc tcggcgacct
     3721 gcgccagacc cagggcgtgg acctcgtccc cggtgagggt ggtggtcgtg taggcgcgga
     3781 taccgaagtc gtagtagccc tcggcgccgg gca
//
LOCUS       DASVTM010000224        11457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_606792,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000224 DASVTM010000000
VERSION     DASVTM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606792"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1136)
                     /locus_tag="VF633_06020"
     CDS             complement(189..1136)
                     /locus_tag="VF633_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX8470650.1"
                     /translation="MFIHNKRLQYTVRVSEPNPALASLLLEQFGGPDGELSAAMRYFT
                     QGLGEVDPGRKDLLIDIATEELSHLEVIGSIVAMLNKGVKAELSEASMAEAELYMALN
                     AGGNSHTQSLLYGGGPALTNSSGVPWTAAYVDSRGDPTCDLRSNIAAESRAKIVYERL
                     INITDDPGIKEALGFLMTREIAHQKSFEKALYAIEDNFPVGKLPGIERFANMYVNTSQ
                     GEGDMEGPWNSGEQWDRIDDLTAVMPADDGDGTASVILNKKTAAVVDKLAARTLSDPA
                     SNPETGVDLGAGPGAGLITGEDLGGAADIDEAAALAKAI"
     gene            complement(1398..1922)
                     /locus_tag="VF633_06025"
     CDS             complement(1398..1922)
                     /locus_tag="VF633_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8470651.1"
                     /translation="MSLIDKALAAVTPIPSQEAREKATLKARQAASAGDWLSLALDHH
                     EQIRTAFAAGREAKSAPERIAAMKALALVLNGHSLAEELVLYPALGQSGEKLHAAQAY
                     LEQTTAKAQMAELENIAPSKPAWLDKWGHIEGAVLTHMYEEEGDWFLKLKEKGENQAR
                     LTARYAEEFERYVG"
     gene            2374..3099
                     /locus_tag="VF633_06030"
     CDS             2374..3099
                     /locus_tag="VF633_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8470652.1"
                     /translation="MRSVRVGVAGWAIPRASAEAFPAEGSGLQRYASEFSAVEINSTF
                     YRSHRASTYVRWRETTPPGFQFALKLPRAITHEARLVGVEALVIAFRKEAELLGDKLG
                     PLLVQLPPSLIFSLAAHGDFFLALRRIWREPVVCEPRHGSWFDPEADALLRAHRVSRV
                     AADPARHPGAGSPGGWRDFAYWRLHGSPRMYSSAYGPAALDALALTIERSQASQNWCI
                     FDNTARGAATADALVLQARINDG"
     gene            complement(3170..5152)
                     /locus_tag="VF633_06035"
     CDS             complement(3170..5152)
                     /locus_tag="VF633_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470653.1"
                     /translation="MSVVQPGGALSMPEAIAGYDWARTPLGERQNWPQALSTALEIML
                     NSRQPMFVAWGDELTFLYNDSYAAILGNKHPRALGKSFRDVWSDIWQDIEPLVRRSLS
                     GQATWSENLPLTMNRHGYPEQTWWTFSYSPLRADADLIAGMFCSCIETTTQVLGQQKA
                     ARDREQLFEMSRDLIGIVSLDGYLISINPAWSRVLGRSTEELLARPFFEIIHSDDLST
                     TTEVVDALAAGRPVDQFMIRLLTAEGAPITFAWSAVPGSVPQERTFYTVGRDITEDLR
                     RDELLRQSQKMEAVGQLTGGLAHDFNNLLAGISGSLELINTRMVQGRTADVVKYVTAA
                     QGAANRAAALTHRLLAFSRRQTLAPRPVDVRRLISGMEDLIGRTIGPQISLETVHAAG
                     LWPSLIDANQLENAVLNLCINARDAMPDGGRITIETANRWLDASAARQRGLSPGQYIS
                     VCVSDTGVGMTPDVQSKAFDPFFTTKPIGVGTGLGLSMIYGFAKQSGGSVSIYSEPGQ
                     GTMVCVYLPRNVGEAEAEVDQAEPISLPRGAGETVVVIDDEPTVRMLIGEVLADLGYN
                     AIEADDGPSGLKVLNSVGRVDLLITDVGLPGGLNGRQVADAARGLRPDLKVLFITGFA
                     ENAVLNHGHLDRGMHVMTKPFAMEALAVRIRELIET"
     gene            complement(5367..5588)
                     /locus_tag="VF633_06040"
     CDS             complement(5367..5588)
                     /locus_tag="VF633_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470654.1"
                     /translation="MKSLLESLRKSDGCYLTEQIPEGFAIVRRDVEYAEAFNALARAT
                     LERSGIDYVALPRTDGPTGYDRVFIIPLN"
     gene            complement(5968..7107)
                     /gene="nagA"
                     /locus_tag="VF633_06045"
     CDS             complement(5968..7107)
                     /gene="nagA"
                     /locus_tag="VF633_06045"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX8470655.1"
                     /translation="MPTTFTNGRILIDGAFREDAAVTIVDDRIVSVGALSATPSGELI
                     DLDGRMLVPGFIDTQVNGGGGVLFNECPTVECIDAIGRAHRQFGTTGFLPTLISDDLS
                     VIDTAIDAVETAIAQGVPGVIGIHIEGPYLNESRKGVHDASKFRTLDAEAIARLTRLK
                     GGRTLVTLAPEMTTPGMISRLVAAGAVVAAGHTDADYAETRAGIEAGMTGFTHLFNAM
                     SPLTSRAPGAVGAALEDINSWCGLIVDGHHVDPVVLKLALRCRPLNRFMLVTDAMPSV
                     GSDEDHFFLQGRRIEVRDGVCRDGDGVLAGSDLDMATAVRNARDLLDLSLEDAVAMAT
                     LWPAQFLGIDDQGKIVRSARANLAILDADLIVTGSWIDGRPVLMR"
     gene            complement(7107..8138)
                     /locus_tag="VF633_06050"
     CDS             complement(7107..8138)
                     /locus_tag="VF633_06050"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX8470656.1"
                     /translation="MRADDTLMFREAADAGRVVAEQLRLNRPVAKALGERLREMNPTG
                     VLICGRGSSDHAGVYGRYLIETGTGLLVSSAAPSIASVYAVTPRLSGMICIAISQSGQ
                     SPDLLASVKAAKNGGALVIALVNVEDSPLARAADVVLPLHAGPERSVAATKSFIASLS
                     AIAQLVGFWLEDEALLDALDALPEQLDVAWRQDWSAALPVLADNENLYVVGRGPGFGV
                     ACEAALKLKETAGLHAEAFSAAEVRHGPMALVKAGFPVLVLPQGDETRADTVALASEF
                     ATRGARVMMAGSGGEARVLTLPTVEAHAALQPILMIESFYRLAAEVSVARGYDPDRPP
                     YLSKVTETV"
     gene            complement(8138..8881)
                     /locus_tag="VF633_06055"
     CDS             complement(8138..8881)
                     /locus_tag="VF633_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8470657.1"
                     /translation="MSFSELIGTLDARRPAPLYQQLQQAVREAIEQRVLKADEALPAE
                     RDLANELSVSRITVRKAFDGLVAEGLLSRRQGAGTFVAARVEKSFSRLTSFSEDMAAR
                     GRKATSSWLNRTEGAVTPEESLTLGLSPGTAVYRYHRIRYADGLPMALEYSTVPASCL
                     PSLMSVEPSLYQALDANGQRPTRALQRLRAVLFTAEQADLLGVAAGAAGLLIERRAFS
                     RTGQAVEVTQSFYRGDAYDFVAELNDGPA"
     gene            9161..>11457
                     /locus_tag="VF633_06060"
     CDS             9161..>11457
                     /locus_tag="VF633_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470658.1"
                     /translation="MVGTVSALALSAGAAMAQDPGPDTVDEVVVTGIRASILESIGTK
                     RDANAIVDVVTAEDVGKFPDKNVAEALQRVPGVVINREFGEGERVSIRGTSPNLTRTL
                     IDGHGLATADWFILEQLSATRSFNFLMLPSEIIGQTLVYKSPMADLEEGGIGGTVDTR
                     TRRPLDLKAFEAFGSLQGAYNENAGDIDPNGSALISWHNEDKTFGVLVAGIYQKRNIR
                     RDGVEVLGYFDADPGAAELLAPSLIGSALFEQERVREGANFAVQWRPTDQMEFALTGL
                     YSRFGADNFNQNFLAWGSNALGGGGTLTNTTIVDGTAVAGTITSTPGGRGAVFDAIDR
                     DAEATTSNIALDGKFTPSDRLTLDFKVGYTQASGDTTNQPFVEFGAPAAFTYDLRGDT
                     PQVTFLNLDPTDPTDMQFDFASLHEVTNDDDEFYGYIDGEYEVDLGPLQSIKVGLKYT
                     DHNRDTDFQATTYGGFFLPLAATGCNGGPCGPSDFAGGLTPNDYLSDIASSGTLDSYW
                     QVDRTKVESILYGLPAATRARVSNYPEIFSVNEKVTGGYVMGKFGGEDWRGNIGVRIV
                     NTDQTSTGYSTSVVASPTDVSNAFGTFAPVSASRSYTDVLPSANFAFDLTDDLVLRFA
                     AARTMARPDFTDVAPRTSLNPGSLTGVGGNPNIDPYRANQFDLSLEYYPEKGTIFSAA
                     YYYKDIESFITDAPSLETFANETSTPNLSLCSFANVAGRPNLYNCQFTINRRANGGGA
                     RLQGLELAVQKSIWRGFGVQANYTYS"
BASE COUNT         2124 a         3614 c         3586 g         2133 t
ORIGIN      
        1 tactgaagtt agctaagccg taacgcacgg aacaggataa ggttacgatt catcgagccg
       61 tcgttgagcc tctccctgat gagggagcga tacgtcagct attcacgccg cacgaagccc
      121 gccacttcga gccagaagca aggacgtcgg caattcgagc cggtccacca gggaccggct
      181 cgataaagtc agatagcctt ggccagtgca gccgcctcat cgatgtcggc ggcgccgccc
      241 aggtcttcgc cggtgatcag acccgcgccg gggccggccc cgaggtcgac gcccgtctcg
      301 ggattgctcg cgggatccga cagggtgcgc gcggccaatt tatccaccac cgctgcagtt
      361 ttcttgttca atatcaccga ggcggttccg tccccgtcgt cagccggcat gacggccgtg
      421 agatcgtcga ttcgatccca ctgctccccg ctgttccagg ggccctccat gtcgccctcg
      481 ccctgagacg tattgacgta catgttagcg aagcgctcga tgccgggcag cttgccgacg
      541 gggaagttgt cctcgatggc atacaaggct ttttcgaacg acttctggtg agcgatctcg
      601 cgggtcatca ggaagccaag cgcttccttg atgccgggat cgtcggtgat gttgatgagc
      661 cgttcgtaga cgatcttggc ccggctctcg gccgcgatat tcgaacgcag gtcgcaggtg
      721 ggatcgcccc gcgagtctac ataggctgcg gtccatggca cgccagacga gttggtcagg
      781 gccgggccgc ctccgtacag cagcgactgg gtgtggctgt taccgcccgc gttgagagcc
      841 atgtagagct ccgcctctgc catggaagcc tcagaaagct cagccttgac gcccttgttc
      901 agcatcgcga cgatcgaacc gatgacttcg aggtggctga gttcctcggt agcgatgtcg
      961 atcagaaggt ccttgcgccc tggatcgacc tcgccgaggc cttgggtgaa gtagcgcatg
     1021 gctgctgaaa gctcgccatc gggtccgcca aactgttcca gcagaagaga ggccaaggct
     1081 ggattgggct ctgaaacccg aacggtatac tgaagtctct tgttgtgaat gaacatgggg
     1141 tgtcctgggg tgtggggaag tccatatttc caacaggcgg agcggctctg cgttccttca
     1201 ctcacggagt aaaacttgat attctataag cagtaaaagt actgtctatt gacggacgcc
     1261 tctgcgagcg cattcggtcc ttcccggatg cgaagctttg ctttatgaaa cttacaaata
     1321 tcgccaagcg ggcagtctgc acttgtgatg ctagtctctc ccttcacgaa gagggatcgc
     1381 cggctccgcc cgttaggtca gcccacgtag cgctcaaact cctcggcgta gcgggccgtc
     1441 agtcgagcct gattttcccc cttttccttc agcttgagga accagtcacc ttcctcctca
     1501 tacatgtggg tcaggacagc cccctcgata tgtccccatt tgtccagcca ggccggcttg
     1561 gacggcgcaa tgttttccag ctcggccatc tgcgccttcg ccgtcgtctg ttcgagatag
     1621 gcttgggcgg catgaagctt ctcgccggac tggcccagcg cggggtaaag caccagttcc
     1681 tccgccagcg aatggccgtt gaggacaagg gcgagagcct tcatcgctgc gatgcgctcg
     1741 ggggcggact tagcctcccg tccggcagca aacgccgtgc ggatttgctc atggtggtcg
     1801 agggcgagcg agagccaatc gcccgccgaa gccgcctgac gcgctttcag cgtggccttc
     1861 tcacgagctt cctgtgaagg gatgggggtg actgcggcga gggctttgtc gattagggac
     1921 atgtgatctc ctggttgtca gtggaaacct tctcggagac gcgagttcct gagtcgggtg
     1981 ccggacatgc cgcgattgct gtcgatcatc gtgtccgcct gctcaggaac gaccggatcc
     2041 gtgctgcatt ctgcagccga aggagcggcg ggatgacgtt attcaccctg agattctaca
     2101 atggcggcga gcgacctctg agagcagact tggtcgagat ggaagatatt ggaagcgccg
     2161 cgaaagtggc ccgggatcgt ctcaggggaa gtcgtttcgc cagcgcgacg gtccatgcgc
     2221 cttctggcgc cataacaagg atcgacgccg cagggatttc gtcgaccgcg cccatcccgt
     2281 atgttccaac gccgcccgct tgaccatgag ccgttcgggt cagaatgcag ttgctgtcgc
     2341 tgaaacttgc cactgtgcac atgagaagcc gcgatgaggt cagtgcgtgt cggtgtcgcg
     2401 ggatgggcga tcccgcgggc ctcagcggag gcctttcctg cagaaggctc gggactgcaa
     2461 cgttacgcca gcgagttttc agccgttgaa atcaacagca cattctaccg cagccatcga
     2521 gcaagcacct atgtccgctg gcgcgagact acaccgcccg gattccagtt cgcgctcaag
     2581 ttgccgcggg caataaccca tgaagcccgg cttgtagggg ttgaggcgct ggtgatcgcc
     2641 tttcgaaagg aggctgaact cctcggggac aagctcggtc cgcttctggt tcaactgccc
     2701 cccagcctga tcttctcgct tgccgcacac ggcgatttct ttctcgccct tcgacgcatc
     2761 tggcgtgagc ccgtagtttg cgagccgcgc catggcagct ggttcgaccc cgaagctgac
     2821 gcgcttcttc gggcgcaccg cgtcagccgg gtcgctgccg accccgctcg gcaccccgga
     2881 gccggttcgc ccggcggttg gcgcgacttc gcttattggc ggctccacgg ctcgccgagg
     2941 atgtattctt ccgcctatgg gccggcggcg ctcgatgctc tcgccctcac catcgagcga
     3001 tcgcaagcat cgcagaattg gtgcatcttc gacaacaccg cccggggcgc cgccactgcg
     3061 gacgccttgg tcctgcaagc ccggatcaac gatgggtaag gtgtggacgg cggttcggca
     3121 gcggcgcttc gcagttctcg gtcttgccga cggcggtcga tgcctgggct caggtttcga
     3181 tcaactcgcg tatgcgcacg gccaacgctt ccatcgcgaa cggcttggtc atcacgtgca
     3241 tgccccgatc aaggtgccca tggttgagca cagcattctc ggcgaagcct gtaatgaaca
     3301 gcaccttgag gtcaggtctc aagccgcggg cggcatcggc tacctggcgt ccgttcagcc
     3361 cgcccggcaa cccgacatcc gtgatcagca ggtccacccg accgaccgag ttcagcacct
     3421 tgaggcctga tggcccatcg tcagcctcga tggcgttgta gccgaggtct gcaagcacct
     3481 cgccgatgag catccgcacg gtgggttcat cgtctatcac cacgaccgtt tcgccagccc
     3541 cacgcggcag agagatgggt tcagcctggt cgacctccgc ttcggcttcg cccacgttcc
     3601 gcggaagata cacgcaaacc atcgtgccct gtccgggttc ggagtaaatg ctgactgacc
     3661 cccctgactg tttggcgaaa ccgtagatca tggagaggcc aaggccggtt cccacgccaa
     3721 tcggtttcgt agtgaagaac ggatcgaaag ccttcgactg aacatctggc gtcatcccca
     3781 cgcccgtgtc cgagacgcac acagaaatgt actgccccgg ggatagccct cgctgtctcg
     3841 ctgcgctggc gtcgagccat cggttggccg tctcaatcgt gatcctgccg ccgtctggca
     3901 tggcgtcacg ggcgtttatg cacaggttga gaacggcgtt ttccagctgg ttggcgtcaa
     3961 tgaggctggg ccacagaccg gctgcgtgca ccgtctcaag actgatctgc ggaccgatcg
     4021 ttcgaccgat cagatcctcc atgccggaga tcaggcgcct cacgtcaacc ggcctgggcg
     4081 caagggtctg ccgtcgcgaa aaggccagga ggcgatgcgt cagcgcggcg gccctgttgg
     4141 cggcgccctg cgcggcggtc acgtacttca ccacgtctgc agttcgcccc tgcaccatgc
     4201 gtgtgttgat gagctccaga gagccggaga tgcccgccag caggttattg aaatcgtgtg
     4261 ccagaccacc agtgagctga cccactgcct ccatcttctg actctgcctg agcaactcat
     4321 cccttctcag gtcttctgtg atgtcacgtc cgaccgtgta gaaagttcgt tcctgaggaa
     4381 cggagcccgg caccgccgac catgcaaacg taatcggcgc gccttcagca gtaagtagcc
     4441 ggatcatgaa ctggtccact ggccgtccgg cggcgagagc gtcgacaacc tccgttgttg
     4501 tcgaaagatc atcggaatgg atgatctcga aaaacggcct ggcgagaagc tcctcggtcg
     4561 atcgccccaa cacgcgcgac caggcaggat tgattgagat gaggtagccg tcgaggctga
     4621 cgatgccgat cagatcccgt gacatttcga agagctgctc acggtcgcgc gctgctttct
     4681 gctgaccgag gacctgcgtc gtggtttcga tgcacgagca gaacattcca gctatcaagt
     4741 ccgcatccgc ccgcagtggg gaatacgaaa aagtccacca tgtctgctct gggtagccat
     4801 gacgattcat cgtcagcggc agattttccg accaggtggc ctggccggac agcgagcgcc
     4861 gaacgagcgg ctcgatgtcc tgccagatgt cggaccagac gtcccggaac gacttgccga
     4921 gtgcgcgagg gtgcttgttg cccaggattg ctgcatagga gtcgttgtac aggaatgtga
     4981 gttcgtcgcc ccaagcgacg aacatcggct gtcgtgagtt gagcatgatc tcgagagccg
     5041 tgctgagtgc ttgcggccag ttctggcgct ctccaagcgg cgtccttgcc cagtcatagc
     5101 cggcgattgc ctctggcatg ctgagcgccc cgccgggttg aacaacgctc aataggcttc
     5161 tccggtcacg tcggtccttc cccccgatcg gggtgtccct gaaacccggc tacgcgatcc
     5221 gcgttcccgt cgacgatgtc gatctgcacc ctggaggggg ccagcgtctg ggacgagggt
     5281 tggtcgttga ccccggccga acaaagcgct cacgacccga ccctgtagtg gggcggcggc
     5341 gaggtacgtt gctgggcacg ctgcagctaa ttgagcggga taatgaatac ccgatcgtat
     5401 ccagtcggcc cgtcggtgcg gggtaacgcg acatagtcta tgcctgagcg ctccagtgtt
     5461 gcgcgcgcca gggcgttaaa ggcctcagcg tactcgacat cccttcggac gatggcgaat
     5521 ccttccggaa tttgctcggt caggtagcat ccatcggatt tgcgaaggga ttccagaagt
     5581 gatttcaatg ggtgctcctc cacaggagca atgttcacga atgtgaacgc cgtttcgaca
     5641 acataggtta acgaagatta aggattcgcg cgctctggtc agcttctacc aagagccagg
     5701 cccatcaatg tgaactgggc cgccccccct ttggaaacgg ggtgcccccg aaccagccac
     5761 gaagggctgt tcctgaggtg tcacaagggg cgcgcgcggg ggcgcatggg cccgctggac
     5821 gatcgccgat taaggcgcgt ctgttccggc gcgcgcgccg caaggcgcgt tcgttctggc
     5881 ggcacaaagc cgcaaaagct ggagttttgt cagaaagccg aggttgcgtc gcacctggta
     5941 gttgggttgg cagaacggtg caggcgtcta cctcatcagc accggtcgtc cgtcaatcca
     6001 ggatcccgta acgatcagat ctgcgtcgag aatagccaga tttgcacgcg cggaacggac
     6061 gatcttgccc tggtcgtcaa tgcccaggaa ctgcgctggc caaagggtcg ccatggcgac
     6121 ggcgtcttca agcgacaggt cgagcaggtc gcgggcgttt cgcaccgccg tcgccatgtc
     6181 gagatctgac cccgccagga ccccgtcacc gtcgcgacaa acgccgtcgc ggacttcgat
     6241 gcgacggccc tgaaggaaga agtgatcctc atccgagccg acgctgggca tggcgtcggt
     6301 gacaagcatg aagcggttca gcgggcgaca ccgtagcgcc agtttcagta ccacgggatc
     6361 gacatggtgg ccgtccacga tcaagccgca ccagctgttg atgtcttcga gcgcggcgcc
     6421 cacggcgccg ggcgcccggc ttgtcagcgg agacatggcg ttgaacaggt gggtgaagcc
     6481 ggtcattccg gcctctatgc ccgcacgagt ctcggcatag tcggcgtccg tgtggccggc
     6541 ggccaccacg gctccagccg cgacgaggcg cgagatcatg cccggtgtcg tcatttccgg
     6601 tgcgagggtg acaagtgtcc gacctccctt caagcgggtc aggcgcgcaa tcgcctcggc
     6661 gtcgagggtc ctgaatttgg acgcatcgtg gacccccttg cgggactcat tgaggtaggg
     6721 accctctatg tggatgccga tgacaccggg aacgccttgg gcgatcgcgg tctccaccgc
     6781 gtcgatggcc gtgtcgatca cagacaggtc atcgctgatc agggtgggca ggaaaccggt
     6841 ggtgccgaac tggcgatggg cgcgaccgat cgcgtcgatg cactcgacgg tcgggcattc
     6901 gttaaacagg acgccgccgc cgccgttgac ctgggtgtcg atgaagccgg gaacgagcat
     6961 ccggccgtca agatcgatca actcgcccga tggtgtggcc gacagggcgc cgacgctgac
     7021 gatgcgatcg tcgactatcg taaccgcagc atcctcgcga aaggcgccgt cgatcagaat
     7081 gcgaccgttg gtgaaggtgg tcggcatcag acggtctcgg ttaccttgct gaggtagggc
     7141 ggtcggtcgg gatcgtagcc tcgcgcgacc gatacttcgg cggccaggcg gtagaagctc
     7201 tcgatcatca ggatcggctg gagcgccgcg tgagcctcga cggtcggcag tgtcaggacc
     7261 cgcgcctctc cgcccgatcc ggccatcatg acgcgcgcgc cgcgcgtcgc gaactcggac
     7321 gccagagcta cggtgtcggc ccgggtctca tcgccctggg gcaagaccag caccggaaag
     7381 ccggccttga ccagcgccat cgggccgtgg cggacctcgg cggcgctgaa ggcttcggca
     7441 tgaaggccag cagtctcttt cagcttcaga gccgcctcgc aagcgacgcc gaagccggga
     7501 ccgcgcccca cgacatacag gttctcgttg tcggccagaa cgggaagggc cgccgaccag
     7561 tcctgacgcc aggcgacgtc aagctgttca ggcagggcat cgagggcgtc cagaagggcc
     7621 tcgtcctcaa gccagaagcc gactaactgg gcgatagccg agaggctggc gatgaatgat
     7681 ttggtggcgg cgaccgagcg ttccggcccg gcatgcagcg gcaggacgac gtccgcagcg
     7741 cgagcgagag gcgagtcctc gacgttgacc aaggcgatga cgagggctcc cccgttcttg
     7801 gctgccttga ccgaggccag cagatcgggg ctttggcccg actgcgaaat ggcgatacag
     7861 atcatgccgg aaagacgagg tgtaacggcg tagaccgagg cgatcgacgg cgcggcggag
     7921 ctgacgagca ggcctgtccc ggtctcgata agatagcggc catagacgcc ggcatggtcg
     7981 gaactgccgc gcccgcagat caggaccccg gtcgggttca tttcgcgcag ccgctcgccc
     8041 aatgccttgg cgaccgggcg gttgaggcgg agctgttcgg ctaccacccg gcctgcatcg
     8101 gcagcctcgc ggaacatcaa ggtgtcatct gcgcgcatca ggcgggaccg tcgttcaact
     8161 cagcgacgaa gtcgtaggcg tcgccgcgat agaaggattg ggtcacctcc accgcctgac
     8221 cggtccggga aaatgcgcgc cgctcgatta gcagcccggc ggcgcccgct gcgacgccca
     8281 gaaggtccgc ctgttcggcg gtgaacagca cggcgcgcag gcgctgcagg gcgcgagtgg
     8341 ggcgttgccc gttggcgtcc agcgcctgat aaagcgacgg ctcgaccgac atcagggacg
     8401 gcaggcacga cgccggcaca gtcgaatatt ccagcgccat cggcaggccg tcggcatagc
     8461 gaatgcggtg atatcgatag acagccgtgc cagggctgag gcccagcgtc agcgattctt
     8521 caggcgtcac cgcgccttcg gtacggttca gccaggagct ggtggccttg cgtccacgcg
     8581 ccgccatgtc ttcggaaaag gacgtcagcc gcgagaagct cttctccacg cgagcggcca
     8641 caaaggtccc cgcgccttgg cgacgcgaca gcaaaccttc ggcgaccaga ccatcgaagg
     8701 ccttgcgaac tgtgatgcgc gacaccgaca gctcgttggc cagatcacgc tcagcgggca
     8761 gggcttcgtc ggccttgagt acacgttgct cgatcgcctc gcgcaccgcc tgttgcagtt
     8821 gctggtacaa cggcgcgggg cgccgtgcgt ccagcgttcc aatcagttca gagaaagaca
     8881 tgggctcccc ggtcgacagg acataaatcg cccaccgctg acgacatcac agcctatcta
     8941 accaaaccaa tcaagaacca atcgtaaaaa agttaagccc aatgtgacga tttgggcttg
     9001 tcagcgatga ttgttcgatg acaggctgcc ctccacagtc gccaaaccgg cggctgccga
     9061 tttcgaacgg cctttattgg tcttctattg gcattgtctg taaccgctgg ggagcggacc
     9121 agacctggag gggtaagtca tgcggtattg tcggacattg atggtcggaa cggtcagcgc
     9181 gctggcgttg agcgctggag cggcgatggc tcaggatccc ggcccggaca cggtggatga
     9241 ggtggtcgtc accggcattc gcgcctccat cctggaatca atcggaacca agcgagacgc
     9301 caatgcgatc gtggatgtcg tcaccgccga agacgtcggc aagtttccgg acaagaacgt
     9361 cgccgaggcg ctccaacgcg ttccgggtgt ggtcatcaac cgcgagtttg gcgagggcga
     9421 gcgggtctcg atccgtggca cctctcccaa cctgacgcgg accctgattg acggccacgg
     9481 cctcgccacc gccgactggt tcatcctcga gcagttgtcg gcgacgcgca gcttcaactt
     9541 cctgatgctg ccctccgaaa tcatcggcca gaccctcgtc tacaagagcc cgatggcgga
     9601 cctcgaggaa ggcggcatcg gcggcacggt cgacacccgc acccgccgtc cgctggacct
     9661 gaaggcgttc gaagccttcg gctctctgca aggcgcctac aacgagaacg cgggcgatat
     9721 cgaccccaat ggctcggcgc tgatcagttg gcacaacgaa gacaagacct tcggcgtgct
     9781 cgtcgcgggt atctaccaga agcgcaacat ccgccgcgac ggggttgagg tgctgggtta
     9841 tttcgacgcc gatcctggag cggctgaact gttggccccg tccctgatcg gctcggccct
     9901 gttcgagcag gagcgggtcc gcgagggcgc gaactttgcg gtccaatggc gccccactga
     9961 ccagatggag ttcgccctga ccggccttta ctcgcggttc ggcgctgata acttcaatca
    10021 gaacttcctg gcctggggtt ccaacgccct cggcggcggc ggcaccctga ccaacaccac
    10081 gatagtcgac ggcacggcgg tggccggcac catcacctcg acgcccggcg ggcgcggggc
    10141 ggtgttcgac gccatcgatc gcgacgccga ggcgaccacc agcaacatcg ctctggacgg
    10201 caagtttacg ccgagcgatc ggctgaccct cgacttcaag gtcggctaca cccaggccag
    10261 cggcgatacg acgaaccagc cgttcgtcga gttcggcgcg ccggcggcct tcacctacga
    10321 cctgcgcggc gacacgcctc aggtgacgtt cctgaacctg gatccgacag atccaaccga
    10381 catgcagttc gacttcgcct cactccacga ggtgaccaac gatgacgatg agttctacgg
    10441 ctatatcgac ggcgagtatg aggtcgatct cgggccgctg cagtcgatca aggtcgggct
    10501 gaaatacacc gaccacaacc gcgacacgga tttccaggcc acgacctacg gcggcttctt
    10561 cctgccgctg gcggcgaccg gttgcaatgg cgggccctgc ggcccgtccg acttcgccgg
    10621 cggactgaca cccaacgact atctcagtga catcgcgtcg agcggcacgc tggactcgta
    10681 ctggcaggtc gatcgcacca aggtcgaaag catcctctac ggcttgcctg cggccacgcg
    10741 ggcccgggtt tcgaactatc cggagatctt ctcggtcaac gagaaggtca cgggcggcta
    10801 cgtcatgggc aagttcggcg gcgaggactg gcgcggcaac atcggcgtcc gcattgtcaa
    10861 caccgaccag acctcgacgg gctacagcac ctcggtggtg gcctcgccca ccgacgtatc
    10921 gaacgccttc ggcacctttg cgccggtttc ggccagccgc agctacaccg acgtgctgcc
    10981 gagcgcgaac ttcgcgttcg acctgactga tgacctggtc ctgcggttcg ccgccgcccg
    11041 cacgatggcg cggcccgact tcaccgacgt cgcaccacgc acctccctga accccggctc
    11101 actgaccggc gttggcggca acccgaacat cgacccgtac cgcgccaatc agttcgacct
    11161 gtctctggaa tactatccgg agaagggcac gatcttctcg gcggcctatt attataagga
    11221 tatcgagagc ttcatcaccg acgcgccatc gctcgagacc ttcgccaacg aaacctcgac
    11281 gccgaacctc tcgctttgca gctttgcgaa cgttgctggt cggcccaacc tctacaactg
    11341 tcagttcacg atcaatcgcc gggccaacgg cgggggcgcg cgtctgcagg gcctcgagct
    11401 ggcggtgcag aagtcgatct ggcgcggctt cggcgttcag gccaactaca cctattc
//
LOCUS       DASVTM010000225         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_182107,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000225 DASVTM010000000
VERSION     DASVTM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182107"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VF633_06065"
     CDS             <1..1021
                     /locus_tag="VF633_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8470659.1"
                     /translation="EGDFAGLDDLFTENPRVVQGMIDIFGAWIDQYGVDGFRIDTARH
                     VNPEFWQAFVPAMLERAKARGIPNFHIFGEVYDHDPAVLARFTQVDRYPAVLDFAFQT
                     TATDVSNGVKGTDALAKLFSADALYAGGEQGAMQLPTFLGNHDMGRIGGFIAKAHPDA
                     SDNELLARATLAHALMMFSRGAPVIYYGDEQGFAGEGGYGAARADMFTSRVAGYASHR
                     HIGGARSPFDTTAPMYQRIAEMARIRAATPALRRGRQIVRAYGDKPGLFAVSRVMEDT
                     RGETLIVYNSSTESLTANIEIATASRNWSSLRGACPVAASAPGSIRVSVPPLDYIICV
                     SEDQE"
     gene            1018..>2584
                     /locus_tag="VF633_06070"
     CDS             1018..>2584
                     /locus_tag="VF633_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX8470660.1"
                     /translation="MNVQTLSHPTTTTTDAATEWWRGAVLYQVYPRSFADSNDDGIGD
                     LPGVTAHLEHIASLGVDGVWLSPFFTSPMKDFGYDVSDYTGVDPIFGTIEDFDALVAR
                     AHDLGLKVIIDQVYSHTSEEHPWFVESRQSKTNAKADWYVWANAKPEGSPPSNWQSVF
                     GGPAWTWDARRGQYYMHNFLKEQPQLAVANPEVQDALIYAMRFWLDRGVDGFRLDAIN
                     FAIHDPKLTDNPPFTSNGKRTRPFDFQDKIHNQSQPEIPLFLSRLRALTDSYPGDRFL
                     VAEVGGDRANEEMKQYTHGSDRMHSAYGFLYLYADHLSAELVEKGPAMWPGVDGEGWP
                     SWTFSNHDAPRAISRWAEGRDEKAFAQMMMLLLMSLRGNVFVYQGEELGLPQAHVPFD
                     RLVDPEAITNWPETLGRDGARTPIPWKADAVQAGFSSVEPWLPIDPRHYPLAVDVQEA
                     DPASILSLSRELIGLRHRYAALRTGGMHMHPVAGSVLSFERGEGDDALLCVFNLGHSP
                     ESWTLPAGWRVVEA"
BASE COUNT          443 a          877 c          798 g          466 t
ORIGIN      
        1 cgagggggac ttcgccgggc tcgacgacct gttcaccgag aacccgcgcg tggttcaggg
       61 gatgatcgac atcttcggcg cctggatcga ccagtatggc gtcgacggct tccggatcga
      121 taccgcgcgc cacgtgaacc ccgagttctg gcaggccttc gtccccgcca tgctggagcg
      181 cgccaaggcc aggggcatcc cgaacttcca catcttcggc gaagtgtacg accacgatcc
      241 ggcggtgttg gcgcggttca ctcaggtcga tcgctatccg gcggtgctgg acttcgcgtt
      301 ccagaccacg gccaccgacg tctccaatgg ggtcaagggc accgacgcgc tggccaagct
      361 gttctcggcc gacgcgttgt atgccggcgg ggaacagggc gcgatgcagt tgccgacgtt
      421 cctcggcaac cacgacatgg gccggatcgg cggcttcatc gccaaggctc atcccgacgc
      481 ttcggacaac gaattgctgg cacgcgctac gctcgcccat gccctgatga tgttttcgcg
      541 cggcgctccg gtcatctatt acggcgacga acagggcttt gctggcgagg gcggttatgg
      601 cgcggcgcgt gccgacatgt tcaccagccg ggtcgcgggc tacgccagcc atcgccatat
      661 cggcggcgct cggtctccct tcgacaccac agcaccgatg taccaacgta tcgccgagat
      721 ggcgcgcatc cgggcggcca ccccagccct tcgccgtggc cgccagatcg tgcgcgccta
      781 tggcgacaag cccgggctgt tcgctgtctc tcgcgtgatg gaagatacca ggggcgagac
      841 cctgatcgtc tacaattcgt cgaccgaatc cctgactgcc aacatcgaga tcgcgaccgc
      901 gtcacgaaac tggtccagct tgcgcggggc ctgtcccgtc gcggcatcgg cgcccggctc
      961 gatccgggtt agcgtccctc ccctcgacta cataatttgc gtatcggaag accaagagtg
     1021 aacgttcaga cccttagcca cccgacgacg acgacgaccg acgcagcgac cgaatggtgg
     1081 cgcggcgcgg tcctgtatca ggtctatccg cgcagcttcg cagacagcaa cgacgacggc
     1141 atcggcgacc tgcccggcgt caccgcacac ctcgaacaca tcgcgtcgct gggcgtcgac
     1201 ggggtctggc tctcgccgtt cttcacctcg ccgatgaagg atttcggcta cgacgtgtcg
     1261 gactacacgg gcgtcgatcc gatcttcggc acgatcgagg atttcgacgc tctggtcgcc
     1321 cgcgcgcacg acctgggcct caaggtcatc atcgatcagg tctattccca tacctccgag
     1381 gaacacccgt ggttcgtcga gagccgccag agcaagacca acgcaaaagc cgactggtat
     1441 gtctgggcca acgccaaacc cgaaggttca cctccgtcga actggcagtc ggtgttcggc
     1501 ggcccggcgt ggacatggga cgcgcggcgc ggccagtact acatgcacaa tttcctcaag
     1561 gagcagcctc agctcgcggt ggccaatcca gaggttcagg acgcactgat ctacgccatg
     1621 cgcttctggc tggatcgcgg cgtggacggc ttccggctcg atgcgatcaa tttcgccatc
     1681 catgatccca aactgaccga caatccgccc ttcacctcga acggaaaacg cacccgtccg
     1741 ttcgacttcc aggacaagat ccacaaccag tcgcagccgg agatcccgct tttcctcagc
     1801 cgcctgcgcg cccttaccga cagctatccg ggcgaccgtt ttctcgtggc cgaggtcggt
     1861 ggggatcgcg ccaatgagga gatgaagcag tacacccacg gctcagaccg gatgcactcg
     1921 gcctatgggt tcctttacct gtacgcggac cacctcagcg ccgaactggt cgagaagggc
     1981 cctgcgatgt ggcccggtgt ggacggcgaa ggctggccgt cctggacctt ctcgaaccat
     2041 gacgccccgc gcgccatctc gcgctgggcc gagggccgtg acgagaaggc cttcgcccag
     2101 atgatgatgc tgttgctgat gtccttgcgc ggcaacgtct ttgtctatca gggcgaggag
     2161 ttgggcttgc ctcaggcgca tgtgccgttc gaccggttgg tcgatcccga agccatcacc
     2221 aactggcccg agacgctcgg ccgcgacggc gcccgcacgc cgatcccgtg gaaggccgac
     2281 gcggttcagg ccgggttctc cagcgtcgag ccctggctcc cgatcgatcc acgtcactac
     2341 ccgctggccg tggacgttca ggaggccgac cccgcctcga tcctgagcct gtcacgcgaa
     2401 ctgatcggcc tgcgccaccg ttatgccgcc ctgcgcaccg gcggcatgca catgcatccg
     2461 gtcgcgggtt cggtgctgtc gttcgagcgt ggcgaaggcg acgatgcgct gttgtgtgtc
     2521 ttcaacctcg gccattcgcc ggagtcatgg acgctccctg ctggctggcg cgtggtcgaa
     2581 gcgg
//
LOCUS       DASVTM010000226         7931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1031919,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000226 DASVTM010000000
VERSION     DASVTM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031919"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF633_06075"
     CDS             complement(<1..563)
                     /locus_tag="VF633_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX8470661.1"
                     /translation="MNILLVGSGGREHALAWKIAASPLTRRLVIAPGNPGMEKLGELR
                     GELRTDDAEGLAALAREIKADLVVVGPEVALAAGLADRLAVAGIPCFGPTMKAAQLET
                     SKAFSKAFLERHEIPTAGYGVYDRVADAKAALNVFKPPYVIKADGLAAGKGVAISPDK
                     RDAEAEIERMLGGRFGSAGSRVVIEEF"
     gene            complement(621..776)
                     /locus_tag="VF633_06080"
     CDS             complement(621..776)
                     /locus_tag="VF633_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470662.1"
                     /translation="MADAPGTEPEDRDPYEENLSRRSEAPAVSPWLIIGLIVMLGVVV
                     YAVSAML"
     gene            839..2455
                     /gene="xseA"
                     /locus_tag="VF633_06085"
     CDS             839..2455
                     /gene="xseA"
                     /locus_tag="VF633_06085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX8470663.1"
                     /translation="MSDDSYVFEGPAEAPAAARDNNPPLSISELSFALKRTLEDRFGH
                     VRLRGEVSKVNRHASGHVYLTLKDDKSAIDGVIWKGAVRGLGVQPESGLEVIVTGKIT
                     SYPARSSYQIVIESMEAAGAGALLAQLERLKTRLHGEGLFEVGRKKPIPTFPATIGVI
                     TSPTGAVIRDILHRIAERWPCHVIVWPVVVQGDSACGQVSNAIRGFDAMTRDGKIPRP
                     DLLIVARGGGSVEDLWCFNDEALARTVAAATIPIISAVGHETDTTLIDFVSDRRAPTP
                     TGAAEIATPVLSDLRFAVADLERRLDRAGSRLLDDRRTRLRAVARGLPTRPEDLVALA
                     QQRLDHASSRLASGLQKNAALHERRLVTVGGRLGRALLDRAIERRGDRLEGVGNRLAA
                     ALQANTTTHERRLLRVSARLTLDPINRRLDQRLAKLNAASAKLTVDRQMERAQTRLSS
                     LLRALATLDPKRPKPGFARIDDETGAMIASASALSPGQAVSLVFPDGSRGAHIDGGDD
                     AVTRPVAAPAVAPRPTPKPKAPPADQGSLF"
     gene            2513..2719
                     /locus_tag="VF633_06090"
     CDS             2513..2719
                     /locus_tag="VF633_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEX8470664.1"
                     /translation="MNAIDPNSGPATLHYGDGEFAVLKPGPYVLCAVSGKTIPLEALR
                     YWSPEHQEAYASPAEALTRMVPAA"
     gene            2716..3585
                     /locus_tag="VF633_06095"
     CDS             2716..3585
                     /locus_tag="VF633_06095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8470665.1"
                     /translation="MILDRRALIAGAGSALIASAVSGCAERALAAPSEGLVLNGQWKQ
                     GGFAFGRTSPRAAIFVDDQPLTVASGSGLFVVGFDRDAGPSTTIEARSGLIRTARTLD
                     IAPGAFPTSSIDGLPPSTIEPNDPALLARIREEAALKAIGFANQIDADDFRDGFVWPL
                     ETYRISSLWGAQRILNGTPARPHYGIDLAAPQGTVIRAPADGVVAFSRSGMHFEGGLV
                     LIDHGQGLITCYLHQSRIEVAVGQKLSRGQEIGCVGMEGRATGPHLCWRMKWRDRNLD
                     PSHMVGANAPGVD"
     gene            3696..4199
                     /locus_tag="VF633_06100"
     CDS             3696..4199
                     /locus_tag="VF633_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470666.1"
                     /translation="MSALQSLSILLVDDNQHMRAITSAILQSAGIRKIREVSDGAMAL
                     EALREHPVDLAIVDFNMFPLDGVEFTRLVRNSPDTANPYLPIIMMTGHSEKHRVMEAR
                     DSGVTEFVVKPITAKAIFDRIQAVIVRPRAFVKTDGYFGPDRRRATTGTYRGEMRRRN
                     DVKVAAS"
     gene            complement(4213..5142)
                     /locus_tag="VF633_06105"
     CDS             complement(4213..5142)
                     /locus_tag="VF633_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8470667.1"
                     /translation="MPGDDHKIPFGQDLIWRLEAAAFAALFALLRALGVERASAFGGW
                     LLRTLGPHVGTHKLVRRNLKIAFPDMPVAERDRLAVEQWDQTGRTFAELAVMDKLTPE
                     GGRVDVVDIERLHAVRDSGRAVVLISGHLANFEVMAAVIMAAGVPCQVTYRAANNPYV
                     DALIRQSRERYGIRLFAPKGDGTRELMAGMKRGDSIALLVDQKYNQGPEVEFFGEPVN
                     ASPGAARLALKFGTIMQPLSVVRLPGVRFRVTAHEPIMVEDTGDKAGDVLRGIQAANT
                     FVEDRVREHPVDWFWVHKRWPDKVYAALGPLDV"
     gene            complement(5132..5902)
                     /locus_tag="VF633_06110"
     CDS             complement(5132..5902)
                     /locus_tag="VF633_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8470668.1"
                     /translation="MELFDLTGKVAVITGSSKGIGKAIAERMAEHGAKVVISSRKAGP
                     CDEVAEAINAKYGEGRAIAVPANIASKEDLQRLVDETNSAFGKIDILVCNAASNPYAG
                     PMAGIADEQFEKIFHNNVLANHWLIQMVAPQMLERKDGSVLIISSIGGLRGNALIGAY
                     NVSKAADMQLARNLAVEWGPSNVRVNCIAPGLIQTDFARYLWENPEILEKTVEPNPMK
                     RIGQPDEIAGTAVYLCSKAAAYVTGQTLVVDGGLTIAW"
     gene            6075..7349
                     /locus_tag="VF633_06115"
     CDS             6075..7349
                     /locus_tag="VF633_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8470669.1"
                     /translation="MRALTTAFLFFGYVFLAMTVGAFLWRMGLGGGAAFAAALGALGV
                     LGAVHLFITGGSQRRALRAEIGQVRDAHRLLADAMESTQGALTELAQAIEAGALNNTE
                     ELTGEVRMLETLIQQMSESIDERLTVAPMIAPNSPDSVRRQQGNMLLKTVHDALSENR
                     VDLYLQPVVSLPQRRTIFYESFTRLRDASDRVMMPAEYLSIAEGEGLVPAIDNLLLFR
                     CAQIVRRLARQDRKVGVFCNVALSSLGDETFFPQFLDFLSENRDLNQALIFELGQATF
                     DARGAVEARNMARLADLGFRFSIDKVQTLDLNFNDLQRSDVRFIKIAADLLIEQLLDL
                     DGGSPIPSLRDIQAADFAQLTRRYGIEVIAEKCENERQIVDILELDISMGQGHLFGEP
                     RAIKEQVLAETDPPADFVRSTLLSAEKRRRFA"
     gene            complement(7346..>7931)
                     /locus_tag="VF633_06120"
     CDS             complement(7346..>7931)
                     /locus_tag="VF633_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEX8470670.1"
                     /translation="DGERTYLWDAQGWTGGDINRFWWKSEGQGAFNGKLEDAEVQALY
                     SRAVLPFWDVQAGVRQTFRPDLKDPTDLVLGVQGLAPYWFEVDAAAFLSTEGDLSARV
                     EVEYDQRVTQKWILQPRAEITLAASDVPEIGVGSGLNALELGLRLRYEIRREFAPYVG
                     VEWSRRFGDTRDLVEARAGEAEDVRAIVGLKAWF"
BASE COUNT         1314 a         2731 c         2514 g         1372 t
ORIGIN      
        1 atgaactcct cgatcacgac gcgcgagccg gcactgccga aacgcccgcc gagcatgcgt
       61 tcgatctcgg cctcagcgtc gcgcttgtcg gggctgatgg ccacgccctt gcccgcagcc
      121 agaccatcag ccttgatcac ataggggggc ttgaagacgt tgagcgcggc cttggcgtcg
      181 gcgacccggt catagacgcc gtagcctgcg gtcgggatct cgtggcgttc gaggaaggcc
      241 ttggaaaagg ccttggaggt ctcgagctgg gccgccttca tcgtcgggcc gaaacagggg
      301 atgcccgcca cggccagacg atccgcgagc cctgctgcga gcgcgacttc gggtccgacc
      361 acgaccagat cggccttgat ttcacgcgca agcgccgcga ggccctcggc atcatccgtg
      421 cgcagttccc cacgcaactc cccgagcttt tccatgcccg ggttgcccgg cgcgatgacc
      481 agccgacggg tcagcggcga ggcggcgatc ttccaggcca gggcgtgttc gcgcccaccc
      541 gatccgacga gcaagatgtt catgggcgcg gaatggccgt ccccgccgct ccggtcaagc
      601 gggcgttggg ctaaccgacg tcagagcatc gccgagacgg cgtagaccac cacgccgagc
      661 atcacgatca acccgatgat cagccaggga ctgacggcgg gcgcctcgct gcgtcgcgac
      721 aggttttcct cgtagggatc gcggtcttcg ggttctgttc cgggagcatc agccatgaag
      781 cgaggataga ccgcacgaac gcttctcgcc aatcacacgt ctgcgctaag gtgtagccat
      841 gagtgacgac tcctatgtat tcgagggccc ggccgaggcg cccgctgctg cgcgggacaa
      901 caacccgccg ctgtcgatca gcgagctgtc gttcgcgctc aagcgcacgc tggaagatcg
      961 cttcggccac gtgcgcctgc ggggcgaggt gtccaaggtc aaccgccacg cctcgggcca
     1021 cgtctatctg acgctcaagg acgacaagtc agccatcgac ggggtgatct ggaagggcgc
     1081 cgtgcgcggc ctgggcgtac agcccgagag cggtctcgaa gtgatcgtca ccggcaagat
     1141 cacctcctat ccggcgcggt cttcctatca gatcgtgatc gagtcgatgg aagcggccgg
     1201 cgctggcgct ctgctggctc aactggaacg cttgaagacc aggctgcacg gcgagggcct
     1261 gttcgaggtc gggcgcaaga agccgatccc gaccttcccc gccaccatcg gcgtcatcac
     1321 cagcccgacc ggggcggtga tccgcgacat cctgcaccgg atcgccgaac gctggccctg
     1381 tcatgtcatc gtctggccgg tggtggttca aggcgacagc gcctgtggtc aggtgtcgaa
     1441 cgccattcgc ggcttcgatg cgatgacaag ggacggcaag atccctcgcc cggacctgtt
     1501 gatcgtagcg cgcggtggcg ggtcggtcga ggacctgtgg tgcttcaacg acgaggctct
     1561 ggcccgcacg gtggccgcgg cgaccatccc gatcatctcg gccgtggggc acgagaccga
     1621 caccacactg atcgacttcg tctccgaccg ccgggccccg accccgaccg gcgccgccga
     1681 gatcgccacc ccggtgctga gcgacctgcg cttcgccgtc gccgatctgg agcgtcgact
     1741 ggaccgtgcc ggatcacggc tgctggacga ccgacgcacg cgtctacggg ctgtggcccg
     1801 tggcctgccc acccgtcccg aagaccttgt cgcgctggcc caacagcggc tggatcatgc
     1861 gtcgagccgc ctggcctcgg gactgcagaa gaacgccgcc ctgcacgagc ggcggctggt
     1921 tacggtcggc gggcgtctgg gacgcgccct gctggaccgc gccatcgagc ggcgtggcga
     1981 ccggctggag ggcgtcggca accggctggc cgccgcgctg caggccaaca ccaccaccca
     2041 tgagcggcgg ctgctgcggg tgtcggcgag actgacgctc gacccgataa atcgccgcct
     2101 tgatcagcgc ttggccaagc tcaacgcagc ctccgccaaa ctgacggtcg atcggcagat
     2161 ggaacgtgcc cagacccggc tgtcctcgct gttgcgcgcg ctcgccaccc tcgatcccaa
     2221 acggcccaag cccggctttg cgcgcatcga cgacgagacc ggcgccatga tcgccagcgc
     2281 gtcggcattg tcgccgggcc aagccgtgtc tctggtcttc cccgacggct cgcgcggcgc
     2341 tcatatcgat ggcggcgacg atgcggtcac ccggcccgtc gcggctccgg cggtggctcc
     2401 tcgccccacg cccaagccca aggcgccgcc cgcagatcaa ggcagcctgt tctaggccgt
     2461 cggtgacgca ggtttgatgg cggcgggcgc cgacccgcgc tatctactgt ccatgaacgc
     2521 tatcgatccc aactccggcc cggccaccct gcactacggc gacggcgagt tcgccgtact
     2581 gaagcccggg ccgtatgtgc tctgcgccgt gtccggcaag accattcccc tcgaagccct
     2641 gcgctactgg tcgccagagc atcaggaggc ctacgccagc ccggccgaag ccttgacccg
     2701 catggtcccc gcggcgtgat cctcgaccgc cgggccctga tcgcgggcgc cggatcggct
     2761 ctgatcgcct ccgctgtttc gggctgcgct gaacgcgcct tggccgcacc ctccgaaggg
     2821 ctggtgctga acggccagtg gaaacagggc gggttcgcct ttggccgcac ctcgccgcgc
     2881 gctgcgatct ttgtcgatga ccagcccctg acggttgcat ccgggagcgg cctgttcgtg
     2941 gtgggattcg accgcgatgc ggggccctcc accacaatcg aggcaaggtc cggcttgatt
     3001 cgcacggccc gcaccttgga catcgccccc ggtgcctttc cgacgagcag catcgatggc
     3061 ctgccgccgt ccaccatcga gcccaacgac cccgccctgc tggcccgcat tcgtgaggag
     3121 gccgcgctca aggccatcgg gttcgccaac cagatcgacg cagacgactt ccgcgacggc
     3181 ttcgtctggc ctctggagac ctatcgcatt tcaagcctgt ggggcgcgca gcggattctg
     3241 aacgggaccc ccgcgcggcc tcactacggt attgacctgg cggccccaca gggcacggta
     3301 attcgtgcgc ccgcagacgg cgtcgtcgcc ttcagtcgca gcggcatgca tttcgagggc
     3361 ggcctcgtcc tgattgatca cggccaaggg ctgatcacct gttacctgca tcaatcgcgg
     3421 atcgaggtag cggtcggtca gaagctgtcg cgcggtcagg aaatcggttg cgtgggcatg
     3481 gaaggccgcg ccacaggtcc gcatctgtgc tggcggatga agtggcgcga tcgcaatctg
     3541 gacccctcac acatggtcgg agcgaacgcc cccggcgtgg actgacaagc ctgaattatc
     3601 gtaattcgag agttgccgag cggatagacc gcaacgtttt cagcgcataa catcacgtgg
     3661 agtccgccgg gctcggcatc acggtcataa cattcatgtc ggcacttcaa tcgctcagca
     3721 tcctgctggt cgacgacaac cagcacatgc gggcgatcac ttcggcgatc cttcagtcgg
     3781 ccggaatccg caagattcgc gaggtgtcgg acggcgcgat ggcgctggag gccctgcgcg
     3841 agcaccctgt cgatctggcc attgtggact tcaacatgtt tccgctggac ggcgtcgagt
     3901 tcacccgtct ggtgcgcaac agtcccgaca ccgcaaatcc ctatctgccg atcatcatga
     3961 tgaccggcca ttcggagaag caccgggtga tggaggcgcg cgattccggg gtgaccgagt
     4021 tcgtggtcaa gccgatcacc gccaaggcga tctttgaccg tattcaggct gtgatcgttc
     4081 ggccccgcgc tttcgtgaag accgacggct atttcggacc cgaccgccgc cgggcgacca
     4141 ccgggaccta tcggggcgag atgcgtcgcc gtaatgacgt gaaggtcgcg gcaagctagt
     4201 cggagcgtca gctcagacgt ccagaggccc cagcgccgcg tagaccttgt ccggccagcg
     4261 tttgtggacc cagaaccagt cgaccggatg ctcgcgcacg cgatcctcga cgaacgtatt
     4321 cgccgcctga atcccgcgca ggacgtcgcc ggccttgtcg ccggtatcct cgaccatgat
     4381 cggctcgtga gccgtcacgc ggaaacggac gcccggaagc cggacgaccg acagcggctg
     4441 catgatagtg ccgaacttca gcgccagccg cgcggctccc ggcgaggcgt tgaccggttc
     4501 gccgaagaac tcgacctccg gtccctgatt atatttctga tcgaccagca gggcgatgga
     4561 gtcgccgcgc ttcatccccg ccatcaactc acgcgtgccg tcgcccttgg gcgcgaacag
     4621 gcggatgccg taacgctcgc ggctctggcg gatcagggcg tcgacatagg ggttgttggc
     4681 ggcgcgatag gtcacctggc acggcacgcc cgccgccatg atgaccgccg ccatcacctc
     4741 gaaattggcc agatggccgg agatcagaac cacggcccgg ccgctgtcgc gcacggcatg
     4801 cagccgctcg atgtcgacca cgtcgacccg accgccctca ggcgtcagct tgtccatcac
     4861 ggccagctcg gcgaacgtcc ggccggtctg gtcccattgc tccaccgcca gccggtcacg
     4921 ctcggcgacg ggcatgtcgg gaaaggcgat cttcagattg cggcggacca gcttgtgcgt
     4981 gcccacgtgc gggccgagcg tgcgcagcag ccagccgccg aacgccgagg cgcgttcgac
     5041 ccccaacgcc ctgagcaggg cgaagagggc tgcgaacgcc gccgcttcca accgccagat
     5101 cagatcctgg ccgaatggga tcttgtggtc gtcaccaggc aatcgtcagt ccgccgtcca
     5161 cgaccagcgt ctggcccgtc acataggccg ccgccttcga acacagataa acagcggtgc
     5221 ctgcgatctc gtccggctgg ccgatccgct tcatcggatt gggctcgacc gtcttctcga
     5281 ggatctccgg gttctcccac aggtatctgg cgaaatcggt ctggatcagg cccggcgcga
     5341 tacagttgac ccgcacgttc gacggtcccc actcgacggc gaggttccgc gccagctgca
     5401 tgtcggcggc cttggacacg ttgtaggcgc cgatcagggc gttgccacgc aagcccccga
     5461 tcgaggagat gatcagcacc gacccgtcct tacgctccag catctgcggt gcgaccatct
     5521 ggatcagcca gtggttggcg aggacgttgt tgtggaagat cttctcgaac tgctcgtcgg
     5581 cgatgcccgc catcggcccg gcgtaggggt ttgaggcggc gttgcagact aggatgtcga
     5641 tcttgccgaa ggccgagttg gtctcgtcga ccagccgctg caggtcttcc ttggaggcga
     5701 tgttggcggg cacggcgatg gcgcggccct ctccatattt ggcgttgatg gcctccgcca
     5761 cttcgtcaca aggcccggcc ttgcgggacg agatcacgac cttggcgccg tgctcggcca
     5821 tgcgttcggc gatggccttg ccgattccct tggacgaacc ggtgatgacg gcgaccttgc
     5881 cggtgagatc gaacagttcc atggcgttac ccctaagtcg ttatgacggt tgtcgtgtgg
     5941 cgtttccggg cttcatcacg ggtttctttc cgcgacgctc tagccgatcc gtcctgatac
     6001 cctgatactc gaccgattcc acccgggaac gtcgtctgac tcgttgtgtc gggggctccc
     6061 aagtctggca gctgatgcgc gcactgacga ccgccttcct gttcttcggc tacgtcttcc
     6121 tggcgatgac ggtcggcgcc tttctgtggc gtatgggtct gggcggcggc gcggccttcg
     6181 cggcggccct cggcgcgctg ggcgtgctgg gcgcggtcca tctgttcatc accggcggat
     6241 ctcagcgccg ggccctccgc gccgagatcg gacaggtccg cgacgctcac cgtctgctgg
     6301 ccgatgcgat ggagtcgacg caaggcgccc tgaccgaact ggcccaggcc atcgaggccg
     6361 gtgccctcaa caataccgag gaactgaccg gcgaggtcag gatgctggaa accctgatcc
     6421 agcagatgag cgagtcgatc gacgaacggc tgaccgtcgc gccgatgatc gcgcccaaca
     6481 gccccgactc cgtgcgccgt cagcagggca atatgctgct caagaccgtg cacgacgccc
     6541 tgtcggaaaa ccgcgtcgat ctgtacctgc agccggtcgt cagcctgccg cagcgccgca
     6601 ccatcttcta cgagagcttc acccgcctgc gcgacgccag cgatcgcgtc atgatgccgg
     6661 ccgaatacct gtcgatcgcc gagggcgagg gtctggtgcc cgccatcgac aacctgctgc
     6721 tgttccgctg cgcccagatc gtgcgtcgtc tggcgcggca ggatcgcaag gtcggcgtgt
     6781 tctgcaacgt cgccctgtcg tcgctgggcg acgagacctt cttcccgcag ttcctcgatt
     6841 tcctgtccga gaaccgcgac ctcaatcagg cgctgatctt cgagctgggt caggcgacgt
     6901 tcgatgcgcg cggcgcggtc gaggcccgca acatggcccg tctggccgac ctcggcttcc
     6961 gcttctcgat cgacaaggtc cagacgctgg acctgaactt caacgacctg cagcgctcgg
     7021 acgtgcgctt catcaagatc gccgccgacc tgctgatcga acagttgctg gatctggacg
     7081 gcggttcgcc tatcccgagc ctgcgcgaca tccaggccgc cgatttcgcc cagctgaccc
     7141 gccgctacgg catcgaggtc atcgccgaga aatgcgagaa cgagcgccag atcgtcgaca
     7201 tcctcgagct cgatatctcg atgggtcagg gccatctgtt cggcgaaccc cgcgccatca
     7261 aggaacaggt gctggctgaa accgacccgc ctgcggactt cgtccgctcg acgcttctgt
     7321 cggctgagaa gcgccgcagg ttcgcctaga accaggcctt gagcccgacg atcgcgcgca
     7381 catcctccgc ctctccggct ctcgcctcga ccagatcacg cgtgtcgccg aagcggcggc
     7441 tccattcgac gccgacgtag ggcgcgaact cgcggcggat ttcgtaacgc aggcgcaggc
     7501 cgagttcgag cgcgttcagg cctgatccaa ccccgatctc aggcacatcg ctcgcggcca
     7561 aggtgatctc agcgcgcggc tgcagtatcc atttctgcgt gactctctgg tcgtattcga
     7621 cctcgacccg cgcactcagg tcgccttcag tcgacaggaa ggcggcggcg tcgacctcga
     7681 accagtaggg cgcgaggccc tgcacgccga gcaccaaatc cgtcgggtcc ttcaggtcag
     7741 gccggaatgt ttgacgcaca cccgcctgca catcccagaa cggcagaacg gcgcggctat
     7801 aaagagcctg aacctcagcg tcctcaagct tgccgttgaa cgcgccctgc ccctccgact
     7861 tccaccagaa gcgattgata tcgccgccag tccagccctg agcgtcccaa aggtaggtcc
     7921 gctcgccatc g
//
LOCUS       DASVTM010000227         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_77112, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000227 DASVTM010000000
VERSION     DASVTM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77112"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VF633_06125"
     CDS             complement(<1..1206)
                     /locus_tag="VF633_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8470671.1"
                     /translation="MPYRAPLRDLAFSLTAIAGVEQLEATGAFPDFDLDLMNAVLEAG
                     GSFSEEVLAPLNRPGDVAGAKYANGSVTAAPGFADAYQQFAAGGWTGLTASVESGGQA
                     LPKALELAAYETVHSANMAFGLCPMLSLAAIEALEAHGTEEQKTVYLPKLVSGEWTGA
                     MVLTEPGAGSDLGALTAAATPNGDGTYALNGQKIYITWGDHDATPNIVHMVLARLPDA
                     PAGPKGISLFLASKFEVKPDGSLGDRNDFRPVGIEHKLGIHASPTCVMSYEGARATLV
                     GQPNQGLSHMFVMMNAARLAVGVEGVGIAEMAYQHALAYALERRQGRSIWTGEANAPI
                     FDHPDIRRMLSVMKAKIEAARGICLSTGVAADLAKHAASEADRKAWKGREDLFTPIAK
                     AWSTDVGCDV"
     gene            1358..1945
                     /locus_tag="VF633_06130"
     CDS             1358..1945
                     /locus_tag="VF633_06130"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8470672.1"
                     /translation="MAEPTIPRRAVLQGAGVLVALMALAGPGTAEVRRWSVDPSQSAI
                     TMRIRAAGLTQTGRFEDWSGDIRFDPTTPESARVAIEVRAASLRMHQTALTTRAAGPE
                     FLDVVRYPAIRFRLTSLQPVSPGRFTARAQVTVKGRTREVVFPAALRVTGDAAQMTGG
                     FALDRAAYDIGTQGPWNALIGRQVRVDVALAVKAA"
     gene            1942..>2087
                     /locus_tag="VF633_06135"
     CDS             1942..>2087
                     /locus_tag="VF633_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX8470673.1"
                     /translation="MTDTIESAAAALRAGRLVLLPTETVYRLAADASDANAVAAIFEA
                     KGRPR"
BASE COUNT          318 a          689 c          751 g          329 t
ORIGIN      
        1 cacgtcgcag ccaacgtcgg tcgaccaggc cttggcgatc ggggtgaaca ggtcctcgcg
       61 gcccttccac gccttgcggt ccgcctcgga ggcggcatgc ttggcaagat cggcagcgac
      121 gccggtggac aggcagatgc cgcgggcggc ctcgatcttc gccttcatca ccgacagcat
      181 gcggcggatg tcggggtgat cgaagatcgg cgcattggcc tcgccggtcc agatcgaccg
      241 gccctgacga cgctccagcg cgtaagccag tgcgtgctga taggccatct cggcaatgcc
      301 gacgccctcg acgccgacgg ccagccgggc cgcgttcatc ataacgaaca tgtgcgacag
      361 gccctgattg ggctggccca ccagagtggc gcgcgcgcct tcataggaca tgacgcaggt
      421 cggcgaggcg tggatgccca gcttgtgctc gatgccgact ggccggaagt cgttgcggtc
      481 gcccagcgat ccgtccggct tgacctcgaa cttggaggcc aggaacaggc tgatgccctt
      541 gggcccggcg ggcgcgtcag gcaggcgagc cagcaccatg tggacgatgt tgggcgtggc
      601 gtcgtggtcg ccccaggtga tgtagatctt ctggccgttc agggcgtatg tgccgtcgcc
      661 gttcggcgta gccgccgccg tcagcgcgcc gaggtcggac ccagcgcccg gctcggtcag
      721 gaccattgcc ccggtccatt cgccgctgac gagcttgggc agatagacgg tcttctgttc
      781 ctcggtgccg tgagcctcaa gcgcctcgat cgccgccagc gacagcatgg ggcagaggcc
      841 gaacgccatg ttggccgagt ggacggtctc atacgcagcc agctccagcg ccttgggcag
      901 ggcctgtccg cctgattcga ccgacgccgt cagaccggtc cagccgcccg ccgcgaactg
      961 ctgataggcg tcggcgaagc ccggagccgc cgtgacggag ccgttggcgt acttggcccc
     1021 tgcgacgtcg ccggggcggt tcagcggagc aagaacctct tccgagaacg agccgccggc
     1081 ctcgagcacg gcgttcatca ggtcgagatc gaagtctggg aacgcgcccg tggcctcgag
     1141 ctgttcgacg cccgcgatgg cggtcaggct gaaggcgagg tcacggaggg gagcgcggta
     1201 gggcattgga actgttcccg gaacggattg gcgtcactgg ctttgacgcc ttgtgccgcc
     1261 atgccgttgc ggcacgcaag cgacttgctg aggatgaacg ccttggcgaa cgagggtggc
     1321 tgggtcagtg tcacccggtc agtggagagg agacgccgtg gctgaaccga cgataccccg
     1381 ccgcgccgtg ctgcagggcg caggagtgct ggtcgcgttg atggcgctgg cagggccggg
     1441 gacggcggag gtccggcgct ggagcgtcga tccgtctcaa tcggccatca ccatgcggat
     1501 ccgggctgcg ggactgaccc agaccggacg gttcgaggac tggtccggcg acatccggtt
     1561 cgatccgacg acgcccgaga gcgctcgcgt ggccatcgag gtccgcgccg cgtcgctgag
     1621 gatgcatcag acggcgctga cgacccgcgc cgccggaccc gagttcctcg acgtcgtccg
     1681 ctatcccgcg atccgcttcc gtcttacatc gctgcagccg gtgtcaccgg ggcggttcac
     1741 ggcgcgggct caggtgaccg tcaaaggccg cacgcgagag gtcgtgttcc cggcggcctt
     1801 gcgcgtgacc ggcgacgcgg cgcagatgac cggcggcttc gccctcgacc gggcggccta
     1861 tgatatcggg acgcagggac cgtggaacgc actgatcggc aggcaggtga gggtggacgt
     1921 ggcgctggcg gtgaaggccg cctgacggac acgatcgagt ccgccgccgc tgcactcagg
     1981 gccggacgtc tggtcctgct gccgaccgag accgtctatc ggctggccgc cgacgcctcc
     2041 gacgccaacg ccgtcgccgc catattcgag gccaagggcc ggccgcg
//
LOCUS       DASVTM010000228         8285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_392577,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000228 DASVTM010000000
VERSION     DASVTM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392577"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VF633_06140"
     CDS             <1..624
                     /locus_tag="VF633_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEX8470674.1"
                     /translation="GARVCVGELSLAAIEAELVQHGIAMATTTESSSLFARAIGPVFD
                     SLPEPIRALHETTGRSVWRGTASVEGAANLVAGLIARVFGFPQDGEGVSVDVCIEAER
                     DQSVWRRNIGASRFLSRLSSPKPGGRVVESFGPLDFDIVLRPEEGRLIYGVEGWRMLG
                     FPMPKAWAPTTATHEQIDAEGRFSFDVEIGLPVVGRMVRYRGWLERG"
     gene            734..1246
                     /locus_tag="VF633_06145"
     CDS             734..1246
                     /locus_tag="VF633_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470675.1"
                     /translation="MKSSSFVLLSLGLLVGCDRSRVNPPTEPAVAVESRAEAAAEAPS
                     DSMGPMLPGAGPTNFVGRWSADPAWCATPQGRNRPLEITTMRFEGYENGCDIARIDQV
                     TGGYQATLSCPAPGGMPKVERVQMSVVGQTLELAYVDRGPRLTNPPIKLTKCTTLGDT
                     STKGPELPMP"
     gene            complement(1265..2359)
                     /gene="ychF"
                     /locus_tag="VF633_06150"
     CDS             complement(1265..2359)
                     /gene="ychF"
                     /locus_tag="VF633_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX8470676.1"
                     /translation="MALKVAIVGLPNVGKSTLFNALTKTAAAQAANYPFCTIEPNTGD
                     VAVPEPRLEALAEIAGSKEIIPSRITFVDIAGLVRGASKGEGLGNQFLANIRDCDAVA
                     FVSRCFVDDDITHVENRIDPISDLEIIETELMLADLESLEKRVVNVEKKAKGGDKDAQ
                     LTLKLINMALVPLRAGKPARTVKVDKEDEKAWHMLQLLTSLPALYVSNVDEASADKGN
                     ELSDLVAKRAAEDNANSVVISAKIDSELAVLDAEEQAEFLESLGLAEPGLNRLIREAY
                     NLLGLQSYFTVGPKEARAWTIHVGDTAPQAAGVIHTDFEKGFIRAETIAFEDFVALRG
                     EAKAREAGKLRAEGKAYVVKDGDVMNFLFS"
     gene            complement(2443..2919)
                     /locus_tag="VF633_06155"
     CDS             complement(2443..2919)
                     /locus_tag="VF633_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX8470677.1"
                     /translation="MKMKIASATLVCLGLALSACNRDGKNSTTASIDTDAGKATVEIT
                     DPWCRPTPNGAQAGACYLTVKSSADNTMTGVATPLAGNAMVHDMSMANGMMTMSDMTG
                     GLPLKAGEKVELKPGGKHLMLTGLTGPLVEGAAVPLTLTFSDTPAMTVQAPVRQPK"
     gene            complement(2927..4267)
                     /locus_tag="VF633_06160"
     CDS             complement(2927..4267)
                     /locus_tag="VF633_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX8470678.1"
                     /translation="MSLKTSAPLSGAYRAVWRWHFYAGLLVLPFLMLMAVTGGLYLFK
                     TEIDGLIYRPLSHVEARPATTSPDRWVASAEAGTGGKVANLIIPARSDEAVRLTINLP
                     DGAKRTAFVDPYDARLIGTTAFGGVMEIIKRLHSLALVGTWANYLVEIVAGWAIILVA
                     TGIFMWWPRGRDVGTATIKASDPKRRPFWRDLHAVTGLYAGAVIAFLVVSGMPWSAFW
                     GDQSMGWVKDHGLGRPKPPVGAFAHAEHQNEPAGVGWTMEGMVMPGDHVMPAPLPASL
                     TRVIATADAVRLPRPYTISIPTDPGLSWTASRVVDRVEDTRILYVDGATGVVRADINW
                     SQFGVGAKAFEWGVAVHQGTQYGWINRYLMLGGCIAIWLLCISGILMWWKRRPPKLSK
                     GRLGAPEAPPGPRVRAAVLGIVLPLAILYPLTGLSLIAALIVDRAVSAARRLAY"
     gene            complement(4343..4744)
                     /locus_tag="VF633_06165"
     CDS             complement(4343..4744)
                     /locus_tag="VF633_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX8470679.1"
                     /translation="MTRSQADDWSIARSLAFLAATFAIVFGSLMPFAALAASTPGDPM
                     VICSAEGPQTISIGVDGADHDKSPSRGMAGAKCAACLLSFAAALPEPPLALKAAGPIT
                     RPASIFIAAGPRVSPPARAPPRPPSTAPPKA"
     gene            complement(4794..5411)
                     /gene="pth"
                     /locus_tag="VF633_06170"
     CDS             complement(4794..5411)
                     /gene="pth"
                     /locus_tag="VF633_06170"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX8470680.1"
                     /translation="MIIIAGLGNPGPKYQNNRHNIGFMAADEIARRWKFGPERAKFQS
                     VISEGEVDSAGGPVKVLLMKPQTFMNESGRAVSEAARFYKIPPADIIIFHDEIDLAPG
                     RFRMKKGGGAAGQNGIRSLISHLGADFRRGRMGVGHPGEAHLVMPHVLGDFHKAEQPW
                     LDALLRACADALPFAATGEDERYQGEVLRLAPAPKFSPRQQARGE"
     gene            complement(5571..6185)
                     /locus_tag="VF633_06175"
                     /pseudo
     CDS             complement(5571..6185)
                     /locus_tag="VF633_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
     gene            complement(6373..7458)
                     /locus_tag="VF633_06180"
     CDS             complement(6373..7458)
                     /locus_tag="VF633_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470681.1"
                     /translation="MSLVHLRRGAAVVAVAVATFLASCSTPEPIAEAPPPPAVLPPPP
                     VTLNASVAEAASVYVTFIRDVGTIRAGFPDAESIQMAMRKGAAYDPAQLSRGMIAYAS
                     ILALQSPEFVMGVRQFGVDPEQRRQVIANIVKDPAYAATLPGADYAAGLIISTLGQDI
                     SSLTTIADAVEGDAYTIQERNDPRRRWAVTPIPNREVRLETAKRISTGQMLPSADESA
                     KLFAAGNSGQGLNLTAGPARAPYTPAVANALAIAALAALGAAGDEARVNTDALTIESN
                     NEFCLSMSKLNLFQCLAASRPSYEDMFCVGRHIVRDLATCALGSTRPVSVSVSEPVDT
                     TNQLAPTVSLPQPGSTASLNQTPYTGN"
     gene            complement(7548..>8285)
                     /locus_tag="VF633_06185"
     CDS             complement(7548..>8285)
                     /locus_tag="VF633_06185"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX8470682.1"
                     /translation="ELLICIDALVRASARRITAVMPYFGYARQDRKTGGRTPISAKLV
                     ANMITRAGADRVLTMDLHAGQIQGFFDIPTDNLVATPVLTQDIKDHYQRGNLMIVSPD
                     VGGVVRARSLAERLNVDLAIVDKRRARAGESEVMNIIGDVSGRQCILFDDIVDSGGTL
                     VNAAKALIEAGATEVSAYISHGVLSGPAVQRVTDGPLKELVITDSIEQPHEVLTCSKI
                     RTVPVAPLIGEAIRRIANEESVSKLFD"
BASE COUNT         1466 a         2461 c         2834 g         1524 t
ORIGIN      
        1 ggcgcgcgag tctgtgtggg cgagttatct ctggccgcaa tcgaggctga actcgtgcag
       61 catgggatcg ccatggcgac gacgaccgag tcgtcgtcgc tgtttgcacg ggcgatcggg
      121 ccggtcttcg atagcctgcc ggaacccatc cgggctttgc acgagaccac tggacgatct
      181 gtctggcgtg ggacggcttc ggtcgagggg gcggcgaacc tggtggcagg gctgatcgca
      241 cgggtgttcg ggtttcctca ggatggagaa ggcgtgtcgg tcgacgtttg catcgaggca
      301 gaacgggacc agtctgtctg gcggcggaat attggcgcca gtcgcttcct cagccggctg
      361 tcttctccca aaccgggcgg gcgagttgtc gagagcttcg gaccgttaga cttcgatatt
      421 gtgttgagac cggaagaggg gcgtctgatc tatggggtag agggctggcg catgctgggc
      481 tttccgatgc cgaaagcgtg ggcccccaca accgctactc acgaacaaat cgatgcggaa
      541 ggcaggttct ccttcgacgt cgagattggg ctgccggtag tgggtcggat ggtccgctat
      601 cggggctggc tggagcgcgg ttgacgggtt gtgccggcgc tgccagttcg cggcgtgcga
      661 tttcaaaact gcagccagtc tgacatcgaa cgatcctcgt tgaacggcga cgctcgagcg
      721 gcgttggtgg gggatgaaaa gctcatcctt cgtgctgctg tcattgggtt tgctcgtggg
      781 ttgcgaccgc agtcgggtta atccgccgac tgaacccgcc gtggccgttg aatcacgggc
      841 ggaagcggcc gcagaagcgc cgtccgactc gatggggcct atgttgccgg gtgcgggtcc
      901 aaccaacttc gtcggtcgct ggagcgctga cccggcttgg tgcgcgactc cgcaaggccg
      961 gaaccgcccg ctcgagatca ccacgatgcg cttcgaaggc tacgagaacg gttgcgatat
     1021 cgctcgcatc gatcaggtaa cgggcggcta tcaggcgacg ctgtcctgtc cggcgccggg
     1081 tggcatgccg aaggtggagc gcgtgcagat gtcggtcgtt ggccagacgt tggaactggc
     1141 ctatgtcgac agagggccgc gcctgacgaa cccgcccata aagctcacga aatgcaccac
     1201 gcttggtgac acctcgacca aggggcctga gctcccgatg ccgtaatgcg gtctagccct
     1261 gaggctagct gaacaggaag ttcattacgt cgccgtcctt gacgacatag gctttgcctt
     1321 cggcgcggag cttgccagcc tcgcgggctt tggcttcgcc acgcagcgcc acaaaatcct
     1381 cgaacgcgat ggtttcggcg cggatgaagc ctttttcgaa gtctgtgtgg atgacgccgg
     1441 cggcttgtgg cgccgtatcc ccgacgtgga tagtccaggc gcgcgcttcc tttggaccga
     1501 ccgtgaagta ggactgcagg cccagcaggt tgtaggcttc ccggatcagg cggttgaggc
     1561 cgggttcggc gaggccgagg ctttcgagaa attcagcctg ttcctcggcg tcgaggaccg
     1621 cgagttccga atcgatcttg gccgagatga ccaccgagtt ggcgttgtcc tcggcggcgc
     1681 gtttggcgac cagatcggag agttcgttgc ctttgtcggc cgaggcctcg tcgacgttcg
     1741 agacgtagag ggcggggagg gaggtcagaa gctgcagcat gtgccacgcc ttctcgtctt
     1801 ccttgtcgac cttgaccgtg cgggcgggct tgccggcgcg aagcgggacc agggccatgt
     1861 tgatcagctt cagcgtaagc tgggcgtcct tgtcgccgcc ttttgccttc ttctcgacgt
     1921 tgacgacgcg cttctcgagg ctttcgaggt cggccagcat caactccgtc tcgatgatct
     1981 cgaggtccga gatcgggtcg atgcggttct cgacgtgggt aatgtcgtca tcgacgaagc
     2041 agcgggacac gaaggccacg gcgtcgcagt cgcggatgtt ggccaggaac tggttgccca
     2101 ggccttcgcc cttggacgcg ccgcggacga gaccggcgat gtcgacgaag gtgatgcgcg
     2161 agggaatgat ctctttcgat ccggcgatct cggccagggc ttcgaggcgg ggctcgggca
     2221 cggcgacgtc gccggtgttc ggctcgatgg tgcagaacgg atagtttgca gcctgtgccg
     2281 ccgccgtctt ggtcagggcg ttgaacaggg tggacttgcc gacgttgggc aggccgacga
     2341 tcgcgacttt cagagccatg gtattcctca gagaaggcgc gagcctttag acggttcgag
     2401 accgtttgtc aggctcggcc agcaaagacc tttgggccgg ccctatttgg gttgccgaac
     2461 cggcgcctga accgtcatgg ctggggtgtc ggagaaggtc agcgtcaggg gtacggctgc
     2521 gccctcgacc agcggcccgg tcaacccggt cagcatcaaa tgcttgccac cgggtttgag
     2581 ttcgaccttt tcaccggcct tgagcggcaa acccccggtc atgtcggaca tggtcatcat
     2641 gccgttggcc atgctcatgt cgtggaccat cgcgttgccc gccagcggcg tggcaacccc
     2701 tgtcatggtg ttgtcggcgg acgacttgac cgtcaggtag caggccccgg cctgggcgcc
     2761 gttgggcgtg ggccggcacc agggatcggt gatctcgacc gtggctttgc cggcgtcggt
     2821 atcgatcgag gcggtggtgg agttcttgcc gtcgcggtta caagcggaca gggccagacc
     2881 cagacaaaca agggtggccg atgcgatttt catcttcatg gaactctcaa taggccagac
     2941 ggcgcgcggc ggaaaccgct cgatcaacga taagggcggc aatcaggctg agacccgtca
     3001 gcggatagag aatggcgagg ggcagcacga tgcccagaac cgcagcgcgg acacgcgggc
     3061 cgggaggcgc ctcgggcgcg ccgagccgcc ccttggacag tttcggaggg cggcgcttcc
     3121 accacatgag gatgccgctg atacacagca accagatagc gatgcagccg ccgagcatca
     3181 gatagcggtt gatccagcca tactgcgtac cctgatggac agcgacgccc cactcgaagg
     3241 ccttggctcc cacgccgaac tggctccagt tgatgtcggc ccgcacgacg ccggtggctc
     3301 cgtcgacata gaggatacgg gtgtcctcga cccgatcaac gacgcgagat gcggtccagc
     3361 tcaggcctgg atcggtcggg atcgagatgg tgtaggggcg tggaagccgc acagcgtcgg
     3421 ctgtcgcgat gaccctggtc agcgacgccg gcagtggagc gggcatgaca tggtcgcccg
     3481 gcatgaccat cccctccatg gtccagccga cacctgccgg ctcgttctgg tgctccgcgt
     3541 gagcgaaggc gccgacgggt ggcttgggtc ggccgaggcc gtgatccttg acccagccca
     3601 tagactgatc gccccagaag gcggaccagg gcatgccgga aaccacgaga aaggcgatga
     3661 ccgctcccgc gtagagtccc gtgactgcgt ggagatctcg ccagaacggg cggcgtttcg
     3721 ggtcgctcgc cttgatcgtc gctgttccga catcgcgacc gcgcggccac cacatgaata
     3781 tccccgtagc caccaggatg atggcccagc ccgcaacgat ctcgaccaga tagttggccc
     3841 atgttccgac gagtgcgagg ctgtgcagac gcttgatgat ctccatcacc ccgccgaaag
     3901 ccgtcgtgcc gatcagtcgg gcgtcgtacg gatcgacaaa ggctgtgcgt ttcgcgccgt
     3961 ccgggaggtt gatcgtcagg cgcaccgcct cgtcagagcg agcggggatt atcaggttgg
     4021 cgaccttgcc tccggttccg gcttcggctg aggcgaccca gcggtcgggt gacgtcgtcg
     4081 ctggacgtgc ttccacatgg gacagggggc gataaatcag gccatcgatt tcggtcttga
     4141 acagatagag accgccggtc acggccatca gcatcaggaa gggcagcacc agcagcccgg
     4201 catagaaatg ccaacgccaa actgctcgat aggcgccgga cagaggggcg gaggtcttga
     4261 gagacatggg ttcggaactc ctgaacatgt atgagccggc gcgcgcagag acgcagcgtc
     4321 gatgggcgtc agtcggggct gttcaggcct tggggggagc ggtggaaggc ggacgtggcg
     4381 gcgcgcgggc cggagggctg acgcgaggcc cggcggcgat gaagatcgag gcagggcgcg
     4441 tgatcggtcc ggccgccttc agggccagcg gtggctcggg cagggctgcg gcgaaagaca
     4501 gcaggcaggc agcgcatttt gccccggcca ttccgcggct ggggctcttg tcgtgatctg
     4561 ccccgtcgac gccgatggat atcgtctgcg gcccttcggc ggagcagatc accatcggat
     4621 cgcccggcgt ggaggccgcc agcgccgcga acggcatcag gctgccgaac acgatggcga
     4681 acgtcgcggc gaggaaggcc agcgagcgcg cgatcgacca gtcgtctgcc tgggagcggg
     4741 tcacgcgcca acgctagctg gaagcgctcc ggtggggaag aggaagcaga gtcctattcg
     4801 ccgcgcgcct gctggcgggg gctgaacttt ggcgcggggg cgagtcgcag aacttcgcct
     4861 tgatagcgct cgtcctcacc ggtcgcggcg aagggcagtg catcggcgca ggcgcgcagc
     4921 agggcgtcca gccaaggttg ttcagccttg tgaaagtccc cgagcacatg cggcatgacc
     4981 agatgggcct cgccggggtg tccgacgccc atacgacccc ggcggaagtc agcgcccaga
     5041 tggctgatca gcgagcggat gccgttctgg ccggcagcgc cgcccccctt cttcatgcgg
     5101 aagcgaccgg gcgcgaggtc gatctcgtca tggaagatga tgatgtcggc gggcgggatc
     5161 ttgtagaagc gtgcggcctc gctgacggca cggccgctct cgttcatgaa ggtctgcggc
     5221 ttcatcagca ggaccttgac cggtccgccg gcgctatcga cttccccctc ggagatcacc
     5281 gactggaatt tggctcgctc gggaccgaac ttccagcggc gcgcgatctc gtcagccgcc
     5341 atgaagccga tgttgtggcg gttgttctgg tatttcgggc ccgggttgcc gaggccggcg
     5401 ataatgatca tgcggtcagg tccggatatg aaaacggccc actcccgtca gggaatgggc
     5461 cgttccgaaa gatatcagac gcgaagcgat caggcttcgg tcttgtcctc gccctcggcg
     5521 tcgtccgatt gctcggtggc gggcacttcg tcggcggcgg cttcggcgac tgcggcctgt
     5581 tcctcggcga tcgtttcttc gtcctgagca gccgagatgg cggccgacga ggtcttgatc
     5641 gaggcgatca cgaagtcacg gtcggtgatg gtgggctcga cgcccttggg cacctgaatg
     5701 tccgagaagc ggatgatgtc gcccagcttg tgaccggcca gatcgacgac caggtcttca
     5761 ggaatgtggt ctgcgcggac cgagatctcg accgtgtgac ggacgatctc gagcgaaccg
     5821 ccctgacgga aggcgccgca tttgtcttcg ttgatgaagt ggacgggcac ttcgatcttg
     5881 atcgtctgct tggcatcgac gcgcatcagg tcgaagtgca ggggacggtc cgacacgggg
     5941 tcgaactgga cgtccttggc gatgaccggc tgggtctcgt cgccgtactt cagcgtgacc
     6001 aggtggccca gcagcttgcc ggtgtacagg ttcttgcgga aatccttctc gttgaccgag
     6061 atggaaacgg gggccttgcc gccgccgtac aggatgccgg gaacggctcc ggagcggcga
     6121 acttcgcgcg cgctgccagt gccggatttc tcgcggacgt ccacgttcag aatgatctca
     6181 gccatgtggc tcgcctcaac aacaaaaacg cctgcgccaa ctgatggctc gcgcggcggg
     6241 gtcatcaacc ctcgaattca agagcgcggg tcattacacg caagatgccc cacacgcaac
     6301 cgcctcgacg gtctgcgacg tcagactctt cgcagtgcgt gatcgatcac ccactgcgaa
     6361 atccgccgcg gtctagttgc cggtgtacgg cgtctggttc aggctggccg tcgagccggg
     6421 ctgcgggagg ctgacggttg gagcgagctg attggtcgta tcgacgggtt ccgagaccga
     6481 gacactaaca ggccgtgtcg aaccgagagc gcaggtcgcg agatcacgca cgatatgacg
     6541 accgacacag aacatatcct cgtagctagg gcgtgaggcc gccagacact ggaacaggtt
     6601 cagcttcgac atgctgaggc agaactcgtt attgctctcg atcgtcaagg cgtcggtgtt
     6661 aacccgagcc tcgtcaccgg ccgctcccag agcagcgagc gcggcgatgg ccaaagcatt
     6721 agccacggca ggcgtgtagg gcgcgcgcgc aggtccagcc gtgaggttca acccttgccc
     6781 cgagttgccc gcggcgaaca atttggcaga ttcatcagcc gacggcagca tctggccggt
     6841 ggaaatccgc ttggcagtct ccaggcggac ctcacgattc gggatcggcg tgacggccca
     6901 gcgacggcgc gggtcgttcc gttcctggat cgtgtaggcg tcgccctcaa cggcatcggc
     6961 gatcgtcgtc agagaggaaa tgtcctggcc aagggttgag atgatcaggc ccgcagcata
     7021 gtccgcgccc ggcaaggtgg cggcataggc ggggtccttg acgatatttg cgatcacctg
     7081 gcgacgttgt tccggatcga cgccgaactg gcgcaccccc attacgaact ccggcgactg
     7141 cagcgcgagg atcgaggcat acgcgatcat gccgcgtgag agctgggccg gatcataggc
     7201 tgcacccttg cgcatcgcca tctggatcga ttccgcgtcc gggaagcctg cgcggatggt
     7261 gccgacgtcg cgaatgaagg ttacatagac cgaagcggcc tcggcaacgc tggcgttcag
     7321 cgtcacgggt ggcggcggca ggacagcggg aggcggcggc gcctcggcga tgggttctgg
     7381 cgtcgaacag ctcgccagaa aagtcgccac tgcgacggcg acaacggcag cgccgcgacg
     7441 aaggtggacc aaagacatca aggttttccc acaggaacgc tgggtgtcgt cgtcgacacc
     7501 gatactcatg agggagttat aacgcctcta aagtggtttt ccgatggtta atcgaacaac
     7561 ttcgataccg attcctcgtt cgctatccgg cggatagctt caccgatgag aggggcgacc
     7621 ggcaccgtac gaatcttcga acaggtcaaa acctcatggg gctgttcgat cgagtccgtg
     7681 atcaccagtt ccttgagcgg gccgtcggta acacgctgga cggcagggcc ggacagtact
     7741 ccgtggctga tgtaggccga gacttctgtc gccccggctt caatcagggc cttggccgca
     7801 ttgacgagag tgccgcccga gtcgacgata tcgtcgaaca ggatgcactg gcggcccgaa
     7861 acgtcaccga tgatgttcat gacctcgctt tcgccggccc tggcgcgtcg tttatcgacg
     7921 atggccagat cgacgttcag cctctcggcg agactgcggg cgcgcaccac gccacccacg
     7981 tccggcgaga cgatcatcag gttgccccgc tggtaatggt ccttgatgtc ctgcgtcagc
     8041 acgggggtgg cgacaagatt atcggtcgga atgtcgaaga agccctgaat ctggcccgcg
     8101 tgcaaatcca ttgtaaggac ccggtcggcg ccggcacggg tgatcatgtt ggccaccagc
     8161 ttggccgaga ttggcgtgcg tccgcctgtc tttcgatcct gtcgcgcata gccgaaatag
     8221 ggcatgacgg ccgtgatccg gcgcgcggac gcccgcacca gagcgtcgat gcagatcagc
     8281 agctc
//
LOCUS       DASVTM010000229         3151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_290313,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000229 DASVTM010000000
VERSION     DASVTM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290313"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VF633_06190"
     CDS             <1..630
                     /locus_tag="VF633_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8470683.1"
                     /translation="KSTLLSLLPRFYDVTDGAITINGVDLRHVSLTDLRDHIGLVTQE
                     PFLFDDSIRANIAYGRGGATTEQIEAAARAAAAHDFIVALPQGYDTRAGEAGMRLSGG
                     QRQRIAIARAFLKNAPILLLDEATSALDTESEALVQAALERLMEGRATLMIAHRLSTV
                     MNADRIHVLEAGRIVETGTHAQLVRKGGLYSRLARQQSLDGTPVVEAVI"
     gene            632..1315
                     /locus_tag="VF633_06195"
     CDS             632..1315
                     /locus_tag="VF633_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8470684.1"
                     /translation="MRPLRNPVVQSILSHVLAGWMSFCFATIRWTYEGRDVAEQVWAE
                     GGGVLCAFWHSRIGLSPSCWPLDVAQPAKALISLSPDGQFIAKAVALQGFPAVRGSSS
                     NKDKAEVAKGGTQALRDGLKQLKIGALAITPDGPRGPVRQMAEGLPLMAKLSKAPVLF
                     IGMSCSPAIRLNSWDRAVLPLPFSKGAVVYDIDLFPAGADLADVAQDWTARLNAVETR
                     ADAITGLKE"
     gene            1312..2598
                     /locus_tag="VF633_06200"
     CDS             1312..2598
                     /locus_tag="VF633_06200"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX8470685.1"
                     /translation="MSPALIAYRLLSRMLEPLAPRLLDARVKQGKEDPVRVDERLGVT
                     RIARPAGELVWLHGVSVGETLSLLPLVERIRRARPDLTVLVTSGTLTSATLLAQRLPV
                     GVIHQFAPVDGPQAVAAFFDHWKPTLGVFVESELWPNLIAEAKARGVKLALVSARITE
                     KTATGWGRFPGAIREILSAFDRIMPQDDASAGRLSHLGGRIDGLVNLKLSGAPLPHDT
                     AAFSRLSAAIGDRPVVVAASTHEGEEIAIVRALDALPVRVCLVIVPRHPKRAGEIATL
                     LTQDGYRFVMRSRGGVLDGGTDLYVADTLGELGLFLRLADVVVMGGSFGPAVGGASVG
                     GHNPLEPARLGKPSVSGPDYLNWIAVTEDLRAAGGLTVVSSPAELASVITPLLDDPSA
                     ARAMGERARKAAADAGAGLDRLWDALQSLLPPGRRL"
     gene            2595..>3151
                     /gene="lpxK"
                     /locus_tag="VF633_06205"
     CDS             2595..>3151
                     /gene="lpxK"
                     /locus_tag="VF633_06205"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX8470686.1"
                     /translation="MKLSTPRWWYIRNSNHARVTRTLLKPASWLWAAVTARRIAKATP
                     VDPGVPVISIGNLTVGGSGKTPVARETLRLLRAAGVEAHALSRGYGGKLEGPLRVDPN
                     VHTAADVGDEPLMLALDSPAWISADRIAGATAALAAGAKALVLDDAHQNMKLKKTLSL
                     IVVDGETRGDEWPFGDGSVFPSGPMR"
BASE COUNT          482 a         1098 c         1061 g          510 t
ORIGIN      
        1 aagagcaccc tgctgagcct tttgccgcgc ttctatgacg tcaccgacgg cgccatcacc
       61 atcaacggcg tcgacctgcg ccatgtcagc ctgaccgacc tgcgcgatca catcggactg
      121 gtgacgcagg agccgttcct gttcgacgac agcatccgcg ccaacatcgc ctatggccgg
      181 ggtggagcca caaccgaaca gatcgaggcc gcagctcgcg ccgccgccgc ccacgacttc
      241 atcgtcgccc tgccccaagg ctatgacacc cgcgccggtg aggccgggat gcggctgtcg
      301 ggcggccagc gccagcgcat cgccatcgcc cgcgccttcc tgaagaacgc cccgatcctg
      361 ctgctggacg aggccacatc ggcgctcgac accgagagcg aggccctggt ccaggcggcg
      421 ctggagcggc tgatggaagg tcgcgccacc ctgatgatcg cccaccgcct gtcgaccgtg
      481 atgaacgccg accgcatcca tgtgctggag gccggacgca tcgtcgagac cggaacgcat
      541 gcccaactgg ttcgcaaagg cggactttac tcgcgtctcg cccggcaaca atcgctggac
      601 ggaacgcccg tcgtcgaagc ggtcatctga agtgaggcct ctccgcaatc ctgtggtgca
      661 gtcgatcctg tcgcatgtgc tggcaggctg gatgtcgttc tgcttcgcca ccatccgctg
      721 gacctacgaa ggtcgcgacg tcgccgaaca ggtctgggcc gagggcggcg gcgtgttgtg
      781 cgccttctgg cactcgcgca tcggcctgag cccgtcgtgc tggcctctcg acgtagctca
      841 gcccgccaag gcgttgattt cgctgtcgcc cgacggtcag ttcatcgcca aggccgtcgc
      901 cctgcagggc tttcccgccg tgcgaggttc atcatccaac aaggacaagg ccgaggtcgc
      961 caagggtggc acccaagccc tgcgcgacgg cctgaaacag ctcaagatcg gcgcactggc
     1021 cattacgccg gacggtcctc gcgggccggt gcgccagatg gccgagggcc tgccgctgat
     1081 ggccaagcta tcgaaagcgc ccgtgctgtt catcggcatg agctgcagcc cggcgatccg
     1141 cctgaacagc tgggaccgcg ccgtcctgcc cctgccgttc agcaagggcg ccgtcgtcta
     1201 tgacatcgac ctgttccctg cgggcgccga tctggccgac gtagcgcagg actggacggc
     1261 gcgcctgaac gcggtcgaga cgcgggcgga cgcaatcacc ggcctgaagg aatgagcccc
     1321 gcgctcatcg cctaccgtct gctcagccgc atgctggagc ctctggcgcc gcgcctgctc
     1381 gatgcgcggg ttaagcaggg caaggaggat ccggtccgag tcgatgagcg gctgggcgtc
     1441 acgcggatag cccggccggc cggcgaactg gtctggttgc acggcgtcag cgtcggcgag
     1501 accctgtcgt tgctgccgct ggttgagcgc atccgccggg cgcgccctga cctgacagtg
     1561 ctggtgacct cgggcaccct gacgtccgcg accctgctgg cccagcggtt gccggtcggc
     1621 gtcatccatc agttcgcgcc ggtcgacggg cctcaggccg tcgccgcatt cttcgaccac
     1681 tggaagccga cgctcggcgt gttcgtcgag agcgagttgt ggcccaactt gatcgcggag
     1741 gccaaggcgc gcggcgtgaa gctggctctg gtcagcgccc ggatcaccga gaagaccgcg
     1801 accggctggg gccggttccc cggcgccatt cgcgaaatcc tgtcggcgtt cgaccgcatc
     1861 atgccccagg acgacgcctc ggccggacgg ttgtcccatt tgggcggacg tatcgacgga
     1921 ctggtcaatc tcaagctctc gggcgccccc ctgccccatg acaccgccgc cttcagccgc
     1981 ctgagcgcgg ccatcggcga tcggccggtg gtcgtcgccg ccagcaccca tgagggcgag
     2041 gagatcgcca tcgtccgcgc cctcgacgcc ctgccggtac gggtctgtct ggtgatcgtg
     2101 ccgcgccacc cgaaacgcgc cggtgagatc gccaccctgt tgacgcagga cggctatcgc
     2161 ttcgtcatgc ggtcgcgcgg cggggtgcta gacggcggca ctgacctgta tgtcgctgat
     2221 accctgggcg agctgggcct gttcctaagg ctggccgacg tggtggtgat gggcggcagc
     2281 tttggaccgg cagtcggcgg cgcctcggtc ggcggccaca atccgctgga accggcgcgt
     2341 ttgggcaagc cgtcggtgtc tggccccgac tatctcaact ggatcgccgt gaccgaagac
     2401 ctgcgcgcgg cgggcgggct gacggtggtg agctctcctg cggaactggc cagcgtcatc
     2461 acacccctgc tggacgatcc ctccgccgcc cgggcgatgg gtgaacgcgc gcgcaaggca
     2521 gccgccgatg ctggagcggg cctcgaccgc ctgtgggacg ctctgcagtc gctactgccg
     2581 cccggacgcc ggttatgaag ctgtcgaccc cgcgctggtg gtacatccgg aacagcaatc
     2641 acgcgcgggt cacgcgcacc ctgctgaagc ccgcctcgtg gttgtgggcc gccgttaccg
     2701 cacgacggat cgcgaaggcg acgccggtcg atccgggcgt cccggtcatc tcgatcggca
     2761 acctgaccgt cggaggttcg ggcaagacgc cggtagcgcg tgagaccctt cggctgcttc
     2821 gggctgcagg cgtcgaggcc cacgccctct cgcgcggcta tggcggcaag cttgagggac
     2881 cattgcgcgt tgatccaaac gtccataccg ccgccgacgt cggcgatgag ccgctcatgc
     2941 tggcgctgga cagtccggcg tggatctcgg ccgaccggat cgcgggcgcg acagcagcgc
     3001 ttgcggcggg agcgaaggcc cttgtgctcg acgacgcgca ccagaacatg aagctgaaga
     3061 agacgctgag cctcatcgtc gtcgatggcg agacgcgcgg agacgaatgg cccttcggcg
     3121 acggctcggt cttcccctcc ggccccatgc g
//
LOCUS       DASVTM010000230         6428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_392717,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000230 DASVTM010000000
VERSION     DASVTM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392717"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VF633_06210"
     CDS             complement(<1..580)
                     /locus_tag="VF633_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8470687.1"
                     /translation="MSIAIETQGLTRRFGRRVAVDGVSMTVPERAVYGFLGRNGAGKT
                     TTLKMLLGLIKPSAGSAFIDGIDVGRDRIGAARRVGALLEAHGFYVNLTGRENLDLTR
                     TLLGVASTEVDRVLEVVEMQDDATRRVSDYSLGMRQRLGLARAMLGAPPVLVLDEPTN
                     GLDPDGIADMRRFLKALPERTGATVLLSSHLLG"
     gene            677..1177
                     /locus_tag="VF633_06215"
     CDS             677..1177
                     /locus_tag="VF633_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX8470688.1"
                     /translation="MAQNDIASFRRLCGRFRWLAIAMVVSVGAVLASVHMIAPLVLGL
                     RGVIPDFGRVILGQIIRVAPSIFYLFGVWSIGRAMGELQKGRLIQPTLARALRSVGLA
                     LGIGSVLQVFVITNLLRLINETRGGYLYWDVGAMTLGMIGGALFLLGRVVDQADRVQS
                     ELDEMI"
     gene            1177..1413
                     /locus_tag="VF633_06220"
     CDS             1177..1413
                     /locus_tag="VF633_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8470689.1"
                     /translation="MPVRVTLDALIVKKGLKARDLAAEVGLSETQLSLFRSGKVKGIR
                     FRTLAKLCAALHCRPGDLLDYDLDPDDLHHPDDE"
     gene            1477..2037
                     /locus_tag="VF633_06225"
     CDS             1477..2037
                     /locus_tag="VF633_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEX8470690.1"
                     /translation="MSTIDDVFDDVQDRATGLLNSEGRSSVHVATGIVLTVGFALLAT
                     ALASGTLKPKRTSRHLRDRAGPVMERPQGAFSLILPAVFSATTLSAVRVWNAPASRER
                     SLAMGLWFAAQTVNAVWLGLRPSSMRSQIAAAMSSAGLAAAFAFEARKLDDGAGKIAA
                     PIGAGTRVANFIQRKTQPAGEGRTVH"
     gene            2141..2710
                     /gene="efp"
                     /locus_tag="VF633_06230"
     CDS             2141..2710
                     /gene="efp"
                     /locus_tag="VF633_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX8470691.1"
                     /translation="MAKINGNTIKPGMVLEHNKGLWVVTKASHVKPGKGGAFANVEAK
                     NLETGNKLNERFRSEDKVERVTLEQKDFSFLYEQGDALVFMDDETYEQIELQKDWVGE
                     DRIPYLQDGMKVVIEMHEERPIGLSLPDQVTVTVAETEPTVKGQTASSSYKPAKADNG
                     VRIMIPPYMGPGERIIVDTATGEYVRRAE"
     gene            2890..3699
                     /locus_tag="VF633_06235"
     CDS             2890..3699
                     /locus_tag="VF633_06235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8470692.1"
                     /translation="MALASALVSVMIDAVRKTARPMLRDFGEVAHLQVSRKGPGDFVT
                     QADIKAEDTLYELLMKARPGYGFLGEERGMMEGTDKSHTWIVDPIDGTTNFMHAMPHF
                     AITVGLQRFAPDGSSEIVAGVTYNPVMNELFWAEKGKGCYLNDQRIRVAGRRDLSESL
                     ISTGLPFIGKPAAAHARSIKDIHAIGQRTAGIRRLGSAALDFAWVACGRYDAYFERNL
                     KPWDVAAGVLFVTEAGGVVTTIDADGDPKTGSSVLASNNELHTQIRKILQA"
     gene            3771..4808
                     /locus_tag="VF633_06240"
     CDS             3771..4808
                     /locus_tag="VF633_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX8470693.1"
                     /translation="MIRAILTALVVLMGTPAMAQNTPLVIAHRGASGERPEHTRAAYE
                     LAIEQGADFVEPDLVMSKDGVFVVRHENEIGGTTDVAAHPEFADRHVTKTIDGVATTG
                     WFTEDFTLAELKTLRARERLPQLRTANTAFDGQYPILTFDEVVEIARAGSARTGRAIS
                     IAPELKHPYYFASIGLPMVDAFVSTLERLNLTGADAPILIQCFEVGPLERLSARVRSP
                     LLQLMVAQGSPVDRPDLSYAAMATPEGLKAIRAYAGSVGVELTMIVPRGPEGSLPATT
                     LIPDAHAAGLKVVAWTFRAENAFLPRELLRGEGFAAHGDLPALFRQFRDLGVDAVFSD
                     FPGIAVETFKR"
     gene            complement(4805..5821)
                     /locus_tag="VF633_06245"
     CDS             complement(4805..5821)
                     /locus_tag="VF633_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX8470694.1"
                     /translation="MRYDFGSDNTAGMAPAALQGVIEANTGYARAYGADETTARAADQ
                     IRQRLDADAEIRFVFSGTAANAIALSMLAFPFEAVLAHHAAHVCTDETGAPGFFGQGL
                     GLIGLPGLSGKIDQIALEAALAEPVVGHRQPAAALSLTQSTEYGTVYSEEELRHLIEP
                     VKAMGYGVHLDGARLANAAAAGFDLTQIPRLGVDILVFGGAKAGANGTEAIVLFDKSL
                     ARRLDNRLKQAGQTASKGRFLAAPFLGLLESNAWEDGAAHANLMAQRLAYGIEAGSPF
                     TLAHPVEANAVFVRMPEAAHQRLNELGWACYRFDDGSVRFVCSWATEVDAVDEVIAAI
                     ASLS"
     gene            complement(5830..>6428)
                     /locus_tag="VF633_06250"
     CDS             complement(5830..>6428)
                     /locus_tag="VF633_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8470695.1"
                     /translation="AGVMLLILRPYRVGDLINISGNIGKVQRLDLFMTQLIDANNVKI
                     VVPNSKVFDDTILNLSGQATRRMELKIGVGYGANLSQARDVLVEAASGMEHVLKDPAP
                     WAGVTDLLDSSVEVTLQAWTLSGDYWQTKADVLQAAKEALDRAGIEIPFPHQVAVPYG
                     PDGAVSVYDEGAAALRRKPGKPTYETSADHRDEDQSEG"
BASE COUNT         1111 a         2317 c         1908 g         1092 t
ORIGIN      
        1 cgcccaacag gtgactggac agcagcaccg tcgcccccgt ccgctccggc agggctttca
       61 ggaaacgccg catgtcggcg atgccgtcag gatcgagacc attcgttggc tcatccagca
      121 ccagcaccgg cggcgcgccc agcatggccc gcgcaaggcc gagccgctgc cgcatgccca
      181 acgaatagtc ggagaccctg cgagttgcgt cgtcctgcat ctcgaccacc tccagcaccc
      241 ggtccacctc ggtcgaggcg acacccagca gggttcgggt caggtcgagg ttctctcgcc
      301 cggtcagatt gacgtagaag ccgtgcgcct ccagcagcgc cccgacccgg cgagcggccc
      361 cgatccggtc gcggcccacg tcgatcccgt cgataaacgc cgacccagcg ctcggtttga
      421 tcaggcccag cagcatcttg agcgtcgtcg tcttgcccgc cccgttacgc ccaaggaagc
      481 cgtagaccgc gcgctcgggc accgtcatcg acaccccatc gacggcgacc cggcggccga
      541 accggcgcgt caggccttgc gtctcgatcg ctatgctcat gtccccgccc gctttcgttt
      601 aactcttgga taaagttgcg cagcgcgcgt ctttaagtca aggttaaaga caggacagga
      661 tcccgaggac gccgacatgg ctcagaacga catcgccagc ttccgacgcc tgtgcggtcg
      721 gttccgctgg ctcgcgatcg cgatggtggt cagcgtcggc gcggtgctgg cttcagtgca
      781 catgatcgcg cccttggtac tgggcttgcg tggcgtgatc ccggacttcg gtcgcgtgat
      841 cctcggccag attatccggg tagcgccctc gatcttctac ctgttcggcg tctggtcgat
      901 cggccgggca atgggagagc tccagaaggg ccgactgatc cagccgacac tggcgcgcgc
      961 actgcgctcc gtaggcctcg cgctcggtat cggcagcgtc ctgcaggtct tcgtcatcac
     1021 caacctgctg cggctgatca acgagacccg gggcggctat ctctattggg acgtcggcgc
     1081 catgacgctc ggcatgatcg gcggcgcgct tttcctgcta ggacgcgtgg tggatcaggc
     1141 cgaccgcgtg cagtccgaac tcgacgaaat gatctgatgc cggttcgcgt aaccctcgac
     1201 gccctgatcg tgaagaaggg cctgaaggcg cgcgacctcg ccgccgaggt cgggctcagc
     1261 gagacgcaac tctccctgtt ccgctcgggc aaggtgaagg gcattcgctt ccgcaccctc
     1321 gccaagctgt gcgccgctct ccactgccgc cccggcgacc tgctggacta cgatctcgat
     1381 cccgacgacc ttcaccaccc ggacgatgaa taggcggaac tgaaagaccc gccgctcatt
     1441 gggcagtcgg gcaggtcaag ggctgaggat cagggtatga gcaccatcga tgacgtcttc
     1501 gacgacgtgc aggaccgggc gaccggcctg ctgaattctg agggccgctc cagcgtccat
     1561 gttgcgaccg ggatcgtact gaccgtaggc ttcgcccttc tggccaccgc cctcgcatcg
     1621 ggaacactca agccgaagcg cacttcccgc cacctccgtg accgcgccgg acctgtgatg
     1681 gaacgcccgc agggggcgtt cagcctgatt cttcctgcgg tcttctcggc cacgacattg
     1741 tcggcggtcc gcgtctggaa tgctccggcg agccgggagc ggtctctggc gatgggactg
     1801 tggttcgccg cccagactgt gaacgccgtg tggctgggcc ttcgcccgtc ctcgatgcgc
     1861 agtcagatcg ccgctgccat gtcttcagcg gggctcgccg ccgcctttgc cttcgaagct
     1921 cgaaagctgg acgacggcgc cggcaagatc gcggcgccta tcggcgccgg gacccgggtc
     1981 gccaacttca tccagcgcaa gacgcagcca gcaggtgagg gccgcaccgt tcactgaaat
     2041 cgctccgggt tgcccttcca cgccacatca tctagggatc gcgccctctt tcgcgcgccc
     2101 gtcaccggac cggctgcgcc cctctgactc agtgaatccc atggcgaaga tcaacggcaa
     2161 caccatcaag ccgggcatgg tgctcgaaca taacaagggc ctgtgggtgg tcaccaaggc
     2221 cagccacgtc aagccaggca aaggcggcgc cttcgccaac gtcgaggcca agaacctcga
     2281 gaccggcaac aagctcaacg agcgcttccg gtcggaagac aaggtcgagc gcgtcacgct
     2341 ggaacagaag gacttttcgt tcctctacga acagggcgac gccctcgtgt tcatggacga
     2401 cgaaacctac gagcagatcg aactgcaaaa ggactgggtt ggcgaagacc gcattcccta
     2461 tctgcaggac ggcatgaagg tcgtcatcga gatgcacgaa gagcgcccga tcggtctgtc
     2521 gctgcccgat caggtcaccg tcaccgtcgc cgagaccgag cccacggtta agggccagac
     2581 cgcgtcgtcg tcctacaagc ccgccaaggc cgacaacggc gtccgcatca tgatcccgcc
     2641 ctacatgggt cccggcgaac gcatcatcgt cgacaccgcc accggcgaat acgtgcgacg
     2701 cgctgaatag gattcaggca gtaggcaata ggcagtaggg attacgcaca ccgccccgtc
     2761 ttccttcctt ccctactgcc tacctgccta ttgcctacct gccccctacc tgcttccccc
     2821 ctttgaactt ctccgtctgt ccgggccttt cccgcctttt caggacacgc ggaccaggag
     2881 cttccgacca tggcattagc ctccgctctc gtttccgtca tgatcgacgc ggtccgcaag
     2941 accgcccgcc cgatgctgcg cgacttcggt gaagtcgctc acctgcaggt ctcgcgcaaa
     3001 ggccccggcg acttcgtcac ccaggccgac atcaaggccg aggacaccct ctacgaactg
     3061 ctgatgaagg cccgccccgg ctacggcttt ctgggcgaag agcgcggcat gatggagggc
     3121 accgacaagt cgcacacctg gatcgtcgac ccgatcgacg gcaccaccaa cttcatgcac
     3181 gccatgcccc acttcgccat cacggtgggc ctgcagcgtt tcgcgcctga cggctcgtcc
     3241 gagatcgtcg ccggcgtgac ctataacccc gtcatgaacg agctgttctg ggccgaaaag
     3301 ggcaagggct gctacctgaa cgaccagcgc atccgcgtcg ccggtcgccg cgacctgtcg
     3361 gaaagcctga tctcgaccgg cctgcccttc atcggcaagc ccgccgccgc ccacgcccgt
     3421 tcgatcaagg acatccacgc catcggccag cgcaccgccg gcatccgtcg cctcggctcg
     3481 gccgcgctgg acttcgcctg ggtcgcctgc ggtcggtacg acgcctattt cgaacgcaac
     3541 ctgaagccct gggatgtcgc tgcaggcgtg ctgttcgtca ccgaggcggg cggcgtggtc
     3601 accaccatcg acgccgacgg cgaccccaag accggctcct cggtgctggc ctcaaacaac
     3661 gaactgcaca cgcagatccg gaaaatcctt caggcttaaa gcccttctcc cgcaggggga
     3721 agaagggagc cagtcaccaa cctgtcgcac ccctgcccta cccatgggcc atgatccgag
     3781 ccatcctgac cgccctcgtt gtgctgatgg gaacgcccgc catggcccag aacactcccc
     3841 tcgtcatcgc ccacaggggc gcatcaggcg agcggcctga acacacccgg gccgcctatg
     3901 agttggccat cgaacagggc gccgatttcg tcgagcccga tctggtgatg tcgaaggacg
     3961 gcgtcttcgt cgtccgacac gagaacgaga tcggcggcac caccgacgtc gcggcccacc
     4021 ccgagttcgc cgaccgccac gtcaccaaga ccatcgacgg cgtcgccacc accggctggt
     4081 tcaccgagga cttcactctc gccgaactga agacattgcg ggctagggaa cggctgccgc
     4141 aactccgcac cgccaacacc gcctttgacg gccagtaccc gatcctgacc ttcgacgagg
     4201 tcgtcgagat cgcccgtgca ggatcggctc gcacgggtcg cgccatctcc atcgcgcccg
     4261 agctcaagca cccgtattat ttcgcctcca tcggcctgcc gatggtggac gccttcgtct
     4321 cgacgctgga gcgcctcaac ctgaccggcg ccgacgctcc gatcctgatc cagtgtttcg
     4381 aggtcggacc gctggaacga ctgtcggccc gggtccgctc gcccctgctg cagctgatgg
     4441 tcgctcaggg ttcgccggtc gatcgtccgg atctgagcta cgccgccatg gccacgcccg
     4501 aagggctgaa ggcgatccgg gcctatgccg gttcggttgg cgtcgagctg acgatgatcg
     4561 ttccgagggg acccgagggc tccctgcccg caaccaccct catcccggac gcccatgccg
     4621 caggactcaa ggtcgtggcc tggaccttcc gtgccgaaaa cgccttcctc ccgcgcgaac
     4681 tgctgcgcgg cgagggcttc gcggcccacg gcgacctccc ggctctgttc cgacagttca
     4741 gggatctcgg cgtcgacgca gtgttctcgg acttccccgg catcgcggtg gaaacgttca
     4801 agcgctagga caggctcgca atcgccgcga tgacctcgtc cacggcatcg acctcagtgg
     4861 cccacgagca cacgaaccgc accgagccgt cgtcgaaccg atagcaggcc cagccgagct
     4921 cattcagccg ctggtgggct gcttcgggca tgcgcacgaa caccgcattg gcctccacgg
     4981 gatgcgccaa cgtaaacggc gaccccgcct cgatcccata ggccaatcgc tgcgccatca
     5041 gattggcgtg cgccgccccg tcttcccacg cattggactc tagcagcccg aggaagggcg
     5101 ccgcgaggaa ccgtcccttg gacgcagtct gcccggcctg tttcagccgg ttgtccagcc
     5161 gccgcgccag cgacttgtcg aacaggacga tcgcctcggt tccgttcgcc cccgccttgg
     5221 cgcccccgaa caccagaata tcgacgccca gacgcgggat ctgcgtcagg tcgaaccccg
     5281 ccgccgccgc attggccagc ctcgccccgt ccagatgcac gccgtacccc atggccttca
     5341 ccggctcgat cagatggcgg agttcttctt cgctataaac cgtgccgtat tcggtggact
     5401 gggtcagcga tagcgccgcc gccggttgcc gatgcccgac aaccggctcg gccagcgcgg
     5461 cctccagcgc gatctggtcg atcttgcccg ataggcccgg caggccgatc agcccgagcc
     5521 cctgtccaaa gaacccaggc gcgcctgtct catcggtgca gacgtgggcg gcgtggtggg
     5581 ccagcacggc ctcgaacgga aacgccagca tcgacagggc gatggcgttg gccgccgtgc
     5641 ccgagaacac gaaccggatc tcggcgtccg catccagccg ctgccggatc tggtcggccg
     5701 cccgcgccgt cgtctcgtcg gcgccgtacg ctcgcgcata gccggtgttg gcctcgatca
     5761 cgccctgaag cgccgccgga gccatcccgg cggtgttatc ggatccgaaa tcgtaacgca
     5821 tcgccagcct cagccttcgc tctggtcttc gtcgcggtga tccgcagacg tctcataggt
     5881 cggtttgccc ggcttgcgcc tcagcgccgc ggccccttcg tcatagaccg aaaccgcccc
     5941 gtccgggcca tagggcaccg ccacctgatg cgggaacggt atctcaatcc cggcgcgatc
     6001 cagcgcttcc ttggcggcct gcaagacgtc ggccttggtc tgccaatagt cgccggacag
     6061 cgtccacgcc tgcagcgtca cctcgaccga actgtccagc agatcggtga cccccgccca
     6121 gggcgccgga tccttcaaca catgctccat cccgctcgcc gcctcgacca gcacgtcgcg
     6181 cgcctggcta agattggcgc cgtaaccgac gccgatcttc agctccatcc gccgcgtcgc
     6241 ctgccccgac aggttcagga tggtgtcgtc gaacaccttg gagttcggca cgacgatctt
     6301 gacgttattg gcgtcgatca gctgcgtcat gaacaggtcc agccgctgca ccttgccgat
     6361 attgccgctg atgttgatca gatcgccgac gcgataggga cgcaggatca acagcataac
     6421 gcccgccg
//
LOCUS       DASVTM010000231         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_22490, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000231 DASVTM010000000
VERSION     DASVTM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22490"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1648)
                     /locus_tag="VF633_06255"
     CDS             complement(260..1648)
                     /locus_tag="VF633_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX8470696.1"
                     /translation="MSPSPFAANLAAVLAVIASIIAFPAMAQLPGADVGALPDPTVVC
                     HVGVYRLDDGSWVDLAPNVNAGLRWRRLDGSSSRMILNTDGHWTSTLGSTDRVEGPQP
                     QLGTCAEGRITFDGQSGRRILLDTQDTTFVGQGGARLKGRLILPPDDGPVPIMIEVHG
                     SEGSNALDFNTFQRFAPASGVGVFVYAKRGSGGSEGHYTQDFHILAEDAAAAVIEARR
                     MAGARAGRVGLHGASQGGWVAPLAASLTPVDFVTVGFGLAYGVLSEDSDQVIQNLEAK
                     GWGPEVLAKAREITDVTGAFVASRGAVGFDGLEAVRARYGAEPWYADIKGEFTGMLLN
                     TSNEQIIAMAPQLDGGTSWDYDPLPVLSSLNTPMLWIQAEKDTGAPPEATRRRLIDLS
                     AQGRPITLLEYPDTDHGIVRFETDPDGHRTSLGYAPGYYQAVLDWARTGRLEGAYGDG
                     RVLARAASLPAH"
     gene            1824..2534
                     /locus_tag="VF633_06260"
     CDS             1824..2534
                     /locus_tag="VF633_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8470697.1"
                     /translation="MLVAIGLLMGFLGPFDSESAPVLLRYLYWMICLIGCGLIGVAAD
                     DWLSRRIPMTWRRVAVVSLLVTPPGSLLVLTTQNVLMGQPFGWQVFLNLLWQVWPIVL
                     AVMAVRSLVWRRMPVRVETRTVIAPPLPEAEAAFRRRLSAKRRTARLIAIEADDHYLK
                     VHTDAGEELVTLRFADALAELAGAYGWRIHRSWWVSAEAVEAVRWRRGVGEMRLVGGL
                     TAPVSRTYAPMLKEAGWS"
     gene            complement(2583..3005)
                     /locus_tag="VF633_06265"
     CDS             complement(2583..3005)
                     /locus_tag="VF633_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8470698.1"
                     /translation="MTNEISTALQAAGLWTGLCVVLLLVLSGLTSVNRRKHSVSIGDG
                     GKHEVAVASRAFGNAIEYMPIGLVAVTLMALTGFPVWTIHLVAGSFFVGRVLHAWGML
                     TTRTNKPPTPGRMLGMLLTYLPLLGAAGALVWRWTLSL"
     gene            complement(3008..>3454)
                     /locus_tag="VF633_06270"
     CDS             complement(3008..>3454)
                     /locus_tag="VF633_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HEX8470699.1"
                     /translation="TVSVDLLRLEDPVVSGRTNEGHVQISVKDVTPEHHDWIYPTGSV
                     RVPTDQPMGDLVVLLLEPQSAESFFAWGMFPEVLNRVEYIEAYAIAPLAEKMLAADPA
                     LKAEFEAKLKSEPAFAADGDARLGWFYERTPFYDQRYRLYPVGREN"
BASE COUNT          610 a         1101 c         1153 g          590 t
ORIGIN      
        1 gagcagaccg ccatgggtgc ggcggtattc caagggaagt gatcagatga cacaggaaca
       61 actggcgggt aggcgcgtcg acgatccggc gcttcgtctg tttgccaccc ccaagctgac
      121 cttggcttgg ttcgtttctg acctcacctc gcccgccgca accacacctc caccgcgatc
      181 aggtttggga cccacgcaag gaacgagatg acgcggtacc aagggagaag gcaggtcaca
      241 ggtggggcgg atgggctgct cagtgcgccg gtaacgacgc agcccgcgcc agaacccgtc
      301 catccccgta tgcaccctcc agccgtccgg ttcgcgccca gtccagcacg gcctgataat
      361 agccgggagc atagccgagc gatgtccggt gaccatccgg gtcggtctcg aaccggacga
      421 tgccgtgatc ggtgtcgggg tattccagca gagtgatcgg gcgtccctga gccgacaggt
      481 cgatcaaccg gcggcgtgtg gcttcgggcg gagccccggt gtccttctcg gcctggatcc
      541 atagcatggg cgtgttcagg ctgctcagaa cgggcagcgg gtcatagtcc cacgacgtcc
      601 cgccatccag ttgcggggcc atggcgatga tctgttcgtt ggatgtgttc aacagcatgc
      661 cggtgaactc gcccttgatg tcggcgtacc agggctcggc gccatagcgg gcgcgaacag
      721 cttccagacc gtcgaacccg accgcgcccc gggaggcgac gaaggcgccg gtgacgtcgg
      781 tgatctcgcg cgccttcgcc agcacctccg ggccccagcc cttggcctcg aggttctgga
      841 tcacctgatc gctgtcttcg gagagtacgc cataggccag accgaagccg accgtgacga
      901 agtcgacggg ggttaggctg gcggcgagtg gcgcgaccca gccgccttga ctcgcgccgt
      961 gcagaccgac ccggccggcc cgtgcaccgg ccatccgtcg ggcctcgatg acggccgcag
     1021 cggcgtcttc ggcgaggata tgaaagtcct gggtgtagtg tccctccgac ccgccggagc
     1081 cgcgcttggc atagacgaac acgccgacgc cagacgccgg agcgaagcgc tggaaggtgt
     1141 tgaagtctag ggcgttggag ccctccgacc cgtgcacctc gatcatgatg ggaaccgggc
     1201 cgtcgtccgg cgggaggatc agccgtccct tgaggcgagc gccgccctga ccgacgaacg
     1261 tggtgtcctg cgtgtcgagc aaaatccggc gacccgactg gccgtcgaac gtgatccggc
     1321 cctccgcgca ggttccgagt tgcggctgcg ggccttcgac acggtcggtc gagccgagcg
     1381 tgctggtcca gtgaccgtcc gtattcagga tcatcctgct gctggacccg tcaagcctgc
     1441 gccagcggag gcccgcgttg acgttcggcg ccagatcgac ccagctcccg tcgtccagac
     1501 gatagacgcc gacatggcag accaccgtcg gatcggggag agccccgaca tccgcgccag
     1561 gaagctgggc catcgcggga aaggcgatga ttgaggcgat gacggccagg acggcagcca
     1621 gattggcggc gaacggggag ggtgacatgg aggttccgat caagcagggc agtttcgacg
     1681 accgccattg ccggtcgcgc tgtggctggc taggcctgcc ttcgcgaacg gcgccgggat
     1741 tgcgcgaagg gggtagtatg aacggtgaga tgaggcagcc ggcacctagg gcggtcatac
     1801 ggcgatgggc gatcgacctc gccatgctcg tcgcgatcgg cctgttgatg gggttcctcg
     1861 gtccgttcga cagcgagagc gcgcccgtac tgctgcgtta tctctactgg atgatctgtc
     1921 tgatcggctg cggccttatt ggggttgcag cggacgattg gctgagccgt cgcatcccca
     1981 tgacgtggcg acgtgtcgcc gtggtctcgc tgctcgtcac gccgccgggc tcattgctgg
     2041 tcctcaccac acagaacgtc ctgatgggtc agcccttcgg ctggcaagtg tttctgaatc
     2101 tgctctggca agtctggccg atcgtgcttg ccgtgatggc cgtccggtcc ctggtctggc
     2161 gacgcatgcc ggtgcgggtc gagacgcgca ccgtcatcgc cccgcctttg cctgaggccg
     2221 aggctgcatt ccggcggcgg ttgtcggcca aacgtcgtac tgcccggctg atcgccattg
     2281 aggccgacga ccactacctc aaagttcaca ccgacgccgg tgaagaactg gtcacccttc
     2341 gcttcgccga cgctctagcg gaactggccg gggcttatgg ctggcgcatc caccgctcat
     2401 ggtgggtatc ggccgaagcg gttgaggctg tccgctggcg tcgaggcgtc ggcgagatgc
     2461 ggctggtggg tgggctgacg gcgccggtaa gccgaaccta cgcaccgatg ctgaaagagg
     2521 cgggctggtc gtgaaacaca gccctctccc tctccaaacg gagaaggaaa atcccgtcgc
     2581 ctctaaagcg acagcgtcca gcgccacacc aacgcccctg cagcgcccag cagcggcagg
     2641 taagtcagca gcattcccag catacgcccc ggagtcggag gcttgttggt gcgggtggtc
     2701 agcattcccc aggcgtgaag gacgcgaccg acgaagaacg acccggcgac cagatggatc
     2761 gtccagacgg gaaaaccggt gagggccatc aaggtcacgg cgaccaaacc gatcggcatg
     2821 tattcgatag cgttgccgaa ggcgcggctg gcgacggcga cctcgtgttt gccgccgtcg
     2881 ccgatcgaaa cggaatgctt gcgtcggttg accgacgtca ggccggagag caccagcaac
     2941 agtacgacac acaggccggt ccaaaggccg gcggcctgca gagccgtaga gatctcgttg
     3001 gtcaaaatca gttctcccgt ccgaccggat acagtcggta gcgctgatca tagaacggcg
     3061 tgcgttcgta gaaccagccc agacgcgcat cgccgtcggc ggcgaacgcg ggctcggatt
     3121 tgagcttggc ttcgaactca gctttcagcg ccggatcggc ggccagcatc ttctcggcca
     3181 gaggcgcgat cgcgtaggcc tcgatatatt cgacgcggtt cagcacctcg gggaacatac
     3241 cccaagcgaa gaagctctcg gccgattgcg gctctagcaa cagcaccacc agatcgccca
     3301 ttggttggtc tgtcggcacc cgcaccgagc cggtcggata gatccagtcg tggtgttccg
     3361 gcgtcacgtc cttgactgaa atctgcacgt ggccctcgtt ggtccggccc gagacgaccg
     3421 gatcctccag ccgcagcagg tcgacggaca cggt
//
LOCUS       DASVTM010000232        18494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_553439,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000232 DASVTM010000000
VERSION     DASVTM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 18494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18494
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553439"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="VF633_06275"
     CDS             <1..1411
                     /locus_tag="VF633_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX8470700.1"
                     /translation="LGALATGAIFSLLKGLDWEEAALLAVVAALLIPCRTAFNRRSRL
                     VEPLRPGWLALLVISVLAAIWLGLFAYRDVEYTDQLWWSFLTDREVSGFLRASAVLAI
                     LTLAIAAVSLLSSPGSGRRRLANADDREKALAALAQADHATPEAWLATLGDKTLLFSP
                     TGRSFIAYRVRGRRWIAMGEPAGLKNERLDLLWAFAELADSYGGSAVFYSVGEGLLAD
                     LATMGLAMHKVGETAVVPVAAFSLEGKAKQNLRTAINRAEREGAGFEVLPVGSATPLA
                     AELKSVSDAWLAHHNGDEKAFSLGRFDPAYLDLTPLAVVRQTSEASPNGRIVAFANLL
                     PGEGDIREVAIDLMRHSPDAPPGVMDYLFIRAIQWSAAEGFDAFDLGMAPLAGLEDRR
                     LAPVFAKVGALVFEEGGALYGFQGLRSYKSKFAPEWRSRFIAAPASTPLPLALLDVAL
                     LTSGGWPGMLGIGKSRRRA"
     gene            1469..2403
                     /gene="cysD"
                     /locus_tag="VF633_06280"
                     /pseudo
     CDS             1469..2403
                     /gene="cysD"
                     /locus_tag="VF633_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516923.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
     gene            2403..4358
                     /gene="cysN"
                     /locus_tag="VF633_06285"
     CDS             2403..4358
                     /gene="cysN"
                     /locus_tag="VF633_06285"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX8470701.1"
                     /translation="MALDDNTYKPSDLIEQDIDAYLTAHQNKSLLRFITCGSVDDGKS
                     TLIGRLLYDSKMIFEDQLAQLEADSKRVGTQGQNIDFALLVDGLTAEREQGITIDVAY
                     RFFATDRRKFIVADTPGHEQYTRNMVTGASTADLAVILVDARKGILTQTRRHSFLVSL
                     MGIRHVVLAVNKMDLVGYDPSVFDAIVADYAAFAAQVGLSNVLAIPLSGLAGDNIASR
                     SENTPWYDGPTLMEHLETVELDADRLQTRPFRMPVQWVNRPNLDFRGFSGLIASGVVK
                     PGDAVRVEPSGRTSTIARIVTKDGDLDEAVAGQSVTLTLTDEVDCSRGDILASATSAP
                     EVADQFETTIVWMADEPLLPGRPYWLKIGARTVSAQITEIKHEINVNTLEERAAKSLP
                     LNGIAVCNLSLDRPIGFDAYSEIPDTGGFILIDRMTNATVGAGMIRFALRRSQNIHWQ
                     ALDVTKATRASDLGQKPAVVWFTGLSGAGKSTIANLVEKKLRFEGKHTYLLDGDNVRH
                     GLNRDLGFTDADRVENIRRVGEVAKLMLDAGLIVLTAFISPFRSERRLARELLGPGEF
                     FEVHVDTPLAIAEARDVKGLYAKARSGALKNFTGVDSPYEAPENPEIVVDTASMTAED
                     AAEMIVERLRSGGYLQAEPQFSEGGGV"
     gene            4554..5744
                     /locus_tag="VF633_06290"
     CDS             4554..5744
                     /locus_tag="VF633_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8470702.1"
                     /translation="MTETLSAAQARRIALAAQGFGGASVTPSPRAFRQTAQRLGVIQI
                     DSVNVVTRTHYLPAFSRLGDYPRDLLETEAWGKKRSLFEYWGHEGSLLPVESQPLFRW
                     RMAAAEAGEGIWGSIARFGHEKSDYVDGILAEIEKRRGVTGGDFADGPRGAPGWWSWS
                     DGKRALEWLFWTGRITTLTRRGFERVYDLPERALPRAVIDAPTPTPEDAQRELIRISA
                     RAMGVATTSDLRDYFRLPLAESRARIHELVEAGELIPVTVQGWNKPAFLDPAARKPRR
                     IEAAALLSPFDNLIWFRERTERLFGVRIRLEIYTPAHKREHGYYVLPLLQNEAITARV
                     DLKSDRKGGALLVQAAWREPDADAETPRILAEQLRSMAGWLGLERVEVAGRGDLAAAL
                     DEAL"
     gene            complement(5741..6835)
                     /locus_tag="VF633_06295"
     CDS             complement(5741..6835)
                     /locus_tag="VF633_06295"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX8470703.1"
                     /translation="MSFLSAFSRTPHPSPVTADDRAILMVDPGFGRVFTDHMVTIRYT
                     AGQGWHSHKVEPRGPIPLDPAASVLHYAQEIFEGMKAYKTDDGRVALFRPEQNARRFQ
                     ASAERLAMPQLPEDMFLEAVREIVKVDADWIPEGEGSLYLRPFMIATEAFLGVRAANE
                     YLFVVLASPVGGYFKGGKNSVTIRVSEHYTRAARGGTGAAKCGGNYAASLVAQAEAVA
                     NGCDQVVFLDAAEGKWIEELGGMNIFFVFKDGSLQTPPLGGTILPGVTRASILQLAKD
                     AGVTVREAPYAFEQWRADVASGEVVEAFACGTAAVLTAISKVVAPGGDFTIGDGKPGP
                     VTTEMLAKLTGIQRGRVADPHNWVQTVIEA"
     gene            7084..7515
                     /locus_tag="VF633_06300"
     CDS             7084..7515
                     /locus_tag="VF633_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX8470704.1"
                     /translation="MKIFTIGYEGATQADVITRLKAAGVDTLIDVRAVAASRRPGFSK
                     TILGASLNAEAIGYRHLRALGTPKPGRDAARKGHVAEMRAIFNDHLTEPAAQLQLAEL
                     RGLAASSKVALLCFEADHAGCHRAVLAERLATEDGFEIVNL"
     gene            complement(7754..8407)
                     /locus_tag="VF633_06305"
     CDS             complement(7754..8407)
                     /locus_tag="VF633_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8470705.1"
                     /translation="MTIELFAHPFSSYCQKVLIAFYENDIPFTYRMMEDPGVGEEFAS
                     LWPIKRFPILREKDRVVLEATTVIEYLQVHHPGPMKLIPEDPDMAVEVRMLDRFFDNY
                     VMTPQGKFVFDALRPAENRDPYGVEQARKLLETSYAWLDERMRDRTWAAGDIFSLADC
                     AAAPSLFYADWTHRIPEHYGYLLAYRARLLRRPSFARAVDDARRFRNYFPLGAPDRD"
     gene            complement(8404..8877)
                     /locus_tag="VF633_06310"
     CDS             complement(8404..8877)
                     /locus_tag="VF633_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8470706.1"
                     /translation="MTVTNSTFTIQRVLNAPPARVFEAYTSVEAKGAWFKAPMDVETL
                     DRDFDFRVGGRERFHARWPDGRLSDFQAVYHDIVENERIILVYDMFHNGDKLSVSLQT
                     LEFKAEDDRTRLIHTEQGSYLVGGAEAVAGREHGTAWGIDNLVALFDDTEAGVVA"
     gene            complement(8874..9218)
                     /locus_tag="VF633_06315"
     CDS             complement(8874..9218)
                     /locus_tag="VF633_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8470707.1"
                     /translation="MLQYESQPLDLAFQALSDPGRRRMVERLSFGPASVSELAEPLPM
                     TLSAVVQHLKVLEEAGLVKSEKIGRVRTCALEIETMTRVEHWIAERRQFWNRQYDQLE
                     AYLAKASEEKDQ"
     gene            complement(9251..9532)
                     /locus_tag="VF633_06320"
     CDS             complement(9251..9532)
                     /locus_tag="VF633_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470708.1"
                     /translation="MNGLKRPEPPTIGALAELLAMDGSTVKADLKPLERAASVTVVVD
                     ENDQRRRRVALTDAGPFATSASLSRSAAGTAIATAERTFRILACTFRSA"
     gene            9755..10105
                     /locus_tag="VF633_06325"
     CDS             9755..10105
                     /locus_tag="VF633_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8470709.1"
                     /translation="MVQYSSSQLTDAFSALADATRRGVLEQLGRTDASITELADKFHM
                     TLTGMKKHVSILEQAGFVTTEKVGRVRTCRLGLRRLEEEASWIETYRQLWTARFEALD
                     QVVEDLKLKETDNA"
     gene            10098..10571
                     /locus_tag="VF633_06330"
     CDS             10098..10571
                     /locus_tag="VF633_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8470710.1"
                     /translation="MPDPTRIDRTSDREFVVTRVFDAPPRIVFQAWTTPELMKQWWAP
                     RSMGVPLLACEMDVRTGGSYRFVFGHDASESMAFVGQYLEVVPPLRLVWTNEEEQDGA
                     VTTVTFEERNGRTHLTVHELYPSKVALEANRGAEEGMPEQFGQLDALLSTLDPAA"
     gene            complement(10685..11374)
                     /locus_tag="VF633_06335"
     CDS             complement(10685..11374)
                     /locus_tag="VF633_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8470711.1"
                     /translation="MSAVRPQILVIDDEPQIHRFLSPALDAAGYDAKRADNGQEGLRG
                     IALWNPDAVVLDLGLPDMDGKEVLRRAREFYAGPIIILSARDKEAEKIAALDLGANDY
                     VEKPFGVGELLARLRAGLRQGALRPVATGVITAGDVSIDLDRRIVMKGEERLKLRPKE
                     YDVLAYLARSAGKVVSHTDLLTAIWGTAHADDVQYLRVVIGQLRHKIEPDPAMPTIVL
                     TEAGIGYRLEG"
     gene            complement(11374..14046)
                     /locus_tag="VF633_06340"
     CDS             complement(11374..14046)
                     /locus_tag="VF633_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEX8470712.1"
                     /translation="MTPVDGAGPEDLRPVSRKRGRLKVFLGMSPGVGKTYEMLRSARR
                     RKAEGGDVLVGVVESHGRKETESLLRGLDVLARNPIDYKGRALMEFDIDAAIARKPGL
                     LLVDEYAHSNVPGSRHPKRWQDVEEILKAGIDVWTTLNVQHLESLSDVVLRITGARQR
                     ETVPDSALSKADDIEVVDITPDELRKRLEEGKVYVPETARLASENFFKIENLTALREL
                     ALRRAAQTVDDQLLVHLREKGVPGPWAAGARILVLIGGDGTAASLVRAGRRLSDMMMD
                     APWTVATVDRGAITGESAARLSDALKLAEQLGARTVTLNGDDVVRAVMDHVSRNHVTQ
                     IVLGKGKTNRLRDLLGRSLAAELLRSASGVAIHIITDEKETSAPAPHQPRPPMRWLGY
                     VVGLAFVFLATATAYVLDTAFERVDLGVIYLAAVVAAGALYGLRPALTAATAAFLIYN
                     FLFLQPRYSFTIGSPTDVLTLIVFWGVALLTGALAGRVREQAKRTQRRAAAVSALLGA
                     SQALAGADDRQATARVLAEQASEAAGAKTVVLLPQDDDLVIAAGAPETPVLGASDMAA
                     ARWAWEHGEAAGHGTGTLPQTGWTFRPLQGVRGRSGVAGIEAEALAPGSDEERLALAL
                     LDQGAVAIERAELAGHAVETETLRRADRFRAVLMNSVSHDLRTPLSTVLGSATTLIDY
                     GKNLTPEVRADLLLSIREEAERLNRYVGDLLDMTRLEGGALKVRAEWTDVRDILNAAA
                     ERVSRRLGQRKMTRDFPGDLSLVKVDEGLLEQALVNVLENAIAYSPDGSTIEIAAYED
                     RNSIVISIEDEGKGIPTAELERVFDKFRRMEESSDRTKGVGLGLAIAKGFVEAMDGRI
                     AAASPIHEGTNGEKPGTRILISLPKAIVTHRELL"
     gene            complement(14057..14638)
                     /gene="kdpC"
                     /locus_tag="VF633_06345"
     CDS             complement(14057..14638)
                     /gene="kdpC"
                     /locus_tag="VF633_06345"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX8470713.1"
                     /translation="MLSQLRPALVMTGLFTLVLGLAYPLAVTGLAQGVFPAQANGSLV
                     RSADGAIQGSSLIAQPFAEARYLHPRPSAAGDGYNAAGSSGSNLGPLNPDLAERVKTD
                     ADAIRADTGATVIPADAVTASGSGLDPDISPAYARLQASRVATARGLTAAQVQGVIDN
                     NTTGALLGFIGQPRVNVLMTNRALDALMPAPAA"
     gene            complement(14651..15409)
                     /locus_tag="VF633_06350"
     CDS             complement(14651..15409)
                     /locus_tag="VF633_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX8470714.1"
                     /translation="MRIQLNSTVAAIALFAVATGVASQAAAQTADADTAQNSSQSIAD
                     SYAPKFAFNVAVGSDYVFRGVSQTEEEPAISGGIDVTSGSFYAGTWASNVSFPGDDDT
                     NAEIDLYGGIRPEFAGFNLDLGVIGYLYTGQADGADYDYVEFKAAASRAFGPATLGAA
                     VYYSPDFFGASEDEATYIEANAAISPADKWTLSGAVGHQFVSSAFDYTTWNVGAAYQL
                     TSNLAVDVRYHDTDEHDFGDIYGSRAVASLKATF"
     gene            complement(15412..15573)
                     /locus_tag="VF633_06355"
     CDS             complement(15412..15573)
                     /locus_tag="VF633_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470715.1"
                     /translation="MSRKSRFKAAGKNDVWFAIFVIAAIVIVGLLANADQTNAPKWPG
                     PVPAEQIKE"
     gene            complement(15585..17660)
                     /gene="kdpB"
                     /locus_tag="VF633_06360"
     CDS             complement(15585..17660)
                     /gene="kdpB"
                     /locus_tag="VF633_06360"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX8470716.1"
                     /translation="MTHANPNIETATGRQPTLAGGFSGAMMGRALGDAFVKLNPRHLV
                     KNPVIFVTWIVALLATVSAAGAIASGPSQGGAAGFAIQLALWLWATVLFANVAESVAE
                     GRGKAAADSLRATRVTTPAKVIDPASGTVSQMPAGDLTVGTIVLVEAGDVIPSDGEII
                     EGVASVNEAAITGESAPVIRESGGDRSAVTGGTTVVSDWLKIRITSAPGSTFLDRMIA
                     MVEGANRRKTPNELALSVLLAGLTLVFLIAVVTLLGLGAYSGVNLDPIVLGALFITLI
                     PTTIGGLLSAVGIAGMDRLLKVNVLATSGRAVEAAGDVDTLLLDKTGTITFGNRMATE
                     VIPVAGVRPEVALRAAVTASLADETPEGRSIVELGRNAGIVVEQPAGSTAIAFSATTR
                     QSGLDAGKDSWRKGAVDAVLKSLGSDTSAPPEFTAAVDRIARSGGTPLAVTENGRLVG
                     VIHLKDVVKPGVKARFAELRAMGLRTVMITGDNPVTAAAIASEAGVDDYLAEATPEDK
                     MRLIKAEQAKGRLVAMCGDGANDAPALAQADVGVAMQTGAQAAREAGNMVDLDSDPTK
                     VIEIVEVGKQLLITRGALTTFSIANDVAKYFAIIPAMFVVALPALSALNVMHLGSPES
                     AILSAVIFNALVIVALIPLALRGVKYRAVGAGKLLARNLLIYGLGGLVAPFVGIKAID
                     LIITGLGLA"
     gene            complement(17677..>18494)
                     /locus_tag="VF633_06365"
     CDS             complement(17677..>18494)
                     /locus_tag="VF633_06365"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX8470717.1"
                     /translation="AGAMYFIETQPAPSLVAAGVDASVNMEGKEVRLGAPSSTVWSVL
                     TTGASSGSVNSMHGSYMPLGGGLQMFLMQLGEILPGGVGSGIAIMVLMAMLSVFVAGL
                     MVGRTPEYLGKKIEAREIQYTMIAVLVLPLAILGFTAISAVFPTAMEGLLNHGPHGLS
                     EMLYAYTSAAANNGSAFAGLTANAPWWNTTLGIGMLMGRFIPAIAVLAIAGSLVVKPK
                     LAPSPGTLPTDGLQFAGLVIGIIIILGGLQFFPALALGPIVEHFQMLNGVTGS"
BASE COUNT         3233 a         5998 c         6113 g         3150 t
ORIGIN      
        1 actgggggct ctcgccaccg gagcgatctt ttccctgctc aagggtctgg actgggaaga
       61 ggccgctctt ctggctgtgg tcgcggcgct gctgatcccg tgccgcacag catttaaccg
      121 gcgctcccga ctggtggagc cgttgcgtcc cggctggctg gccttgctgg tgatttccgt
      181 gctggccgcc atctggcttg gcctcttcgc ctatcgcgat gtcgaatata ccgaccaact
      241 gtggtggagt ttcctgaccg accgcgaagt ctccggcttc ctgcgggcga gcgccgtcct
      301 cgccatcctg accctcgcca tcgcggccgt gtcgctgctg tcctcgcccg gctcaggtcg
      361 acgccggctc gccaatgccg acgaccgcga aaaagcgctc gcggctctgg cgcaggccga
      421 ccacgcaacg cccgaagcct ggctcgcgac gctgggtgac aagacgttgt tgttcagtcc
      481 aacgggccgc agcttcatcg cctatcgcgt acgcgggcgt cgctggatcg ccatgggtga
      541 acctgcaggc ctgaagaatg agcgcctcga cctgctctgg gccttcgccg aactggccga
      601 cagctacggc ggctcggcgg tcttctactc ggtcggcgag ggtctgctgg ccgatctggc
      661 caccatgggg ctggccatgc acaaggtcgg cgagacagcc gtagtgccgg tcgccgcctt
      721 ctcgctcgaa ggcaaggcca agcagaacct gcgcaccgcc atcaatcgcg ccgaacgcga
      781 gggcgccggc ttcgaggttc tccccgtcgg atcggcgacc ccgttggccg ccgaacttaa
      841 atccgtgtca gacgcctggc tcgcgcatca caacggcgac gaaaaggcct tttcgctcgg
      901 tcggttcgac cccgcttatc tggacctcac ccccctcgcc gtcgtgcgcc agacctcgga
      961 ggcatcaccg aatggccgga tcgtcgcctt cgccaacctc ttgccgggtg agggtgacat
     1021 ccgtgaggtg gccatcgacc tgatgcgcca cagccccgac gcgcctccgg gcgtgatgga
     1081 ttatctgttc attcgcgcca tccagtggtc tgcagcagaa ggctttgacg ccttcgacct
     1141 cggcatggcg cctctcgcag gactggagga ccgccgcctt gcccccgtct tcgccaaggt
     1201 cggcgctctc gtgttcgagg aaggcggcgc gctttacggc tttcagggcc tgcgcagcta
     1261 caagtcaaaa ttcgcccccg aatggcgctc gcggttcatc gccgcccccg cgtccacgcc
     1321 cttgccgctc gctcttctgg atgttgcgct gctgaccagc ggcggttggc ccggcatgtt
     1381 gggaatcggc aaatcccgcc gcagggcgta agcagcgcta gggtctggct tcagcttatt
     1441 tcgtgagggt gcggcgccgc acccggctat gtccgcccct caagtttcga ggaccacgcc
     1501 ttgccgaccc tgacccacct ccagcgcctg gaggccgaga gcatccacat cctgcgcgag
     1561 gtcgtcgccg aggccgagcg cccggtgatg ctctattccg tgggcaagga ttcggccgtg
     1621 atgctgcacc tcgcagccaa ggcgttctgg ccttctccgc cgcccttccc gctgctgcac
     1681 gtcgatacga cgtggaaatt ccgcgccatg taccagatgc gcgagcggat ggccgccgaa
     1741 ctcggcttcg agcttctggt ccaccggaac ccggatgcgc tggccaaggg catcaacccc
     1801 ttcgaccacg ggtcggcgct ccacaccgac ctctggaaaa ccgaaggcct gaaacaggcc
     1861 ctcgacctgt acggtttcga tgtcgccttc ggcggcgccc gccgtgatga ggagaaatcc
     1921 cgcgccaagg aacgcatctt ttcgttccgc tccgccggtc accgctggga tcccaagcag
     1981 cagcggcccg agctctggaa gctctacaat gcgaagaaga gcccgggcga aagcatccgc
     2041 gtcttcccga tctcgaattg gaccgagctc gacgtctggc aatacatcca tctggagaac
     2101 atcccgatcg tgccgctcta ctatgccgcg gaacggccgg tggtggagcg cgacggtcag
     2161 ttgatcatgg tcgacgacga ccggatgcgg ctccatcccg gcgagacgcc gcagctgaag
     2221 tcggtgcggt tcaggacgct gggcgactat ccgctgacgg gtgcggtgga gagcgccgcc
     2281 gataccctcc ccgccatcat tcaggagatg ctgctgacca cgacctcgga acgtcaaggt
     2341 cgcgccatcg accacgatca ggccgggtcg atggagaaga agaagcagga gggctatttc
     2401 tgatggcgct cgacgataac acctacaaac cgtccgacct gatcgaacag gacatcgacg
     2461 cctatctgac cgcgcatcaa aacaagagcc tgctgcgctt tatcacctgc ggctcggtgg
     2521 atgacggcaa gtcgaccctg attggacgac tgctgtacga ttcgaagatg atcttcgaag
     2581 atcagctggc ccagttggaa gcggactcca aacgcgtcgg cacccagggc cagaacatcg
     2641 atttcgccct tctggtcgat ggcctgaccg ccgagcggga acagggcatc accatcgacg
     2701 tggcttaccg gttcttcgcc accgacagac ggaagttcat cgtcgccgac acgccggggc
     2761 atgagcaata cacccgcaac atggtcacgg gcgcctcaac cgccgacctc gccgtcatcc
     2821 tcgtggatgc gcgcaagggt atcctgaccc agacccggcg gcacagtttc ctagtctccc
     2881 tgatgggcat ccgccacgtc gtcctggccg tgaacaagat ggatctggtc gggtatgacc
     2941 cgtcggtgtt cgacgccatc gtggcggact acgccgcctt cgctgcgcag gtcggtctgt
     3001 ccaacgtgct cgccatcccg ctgtccgggc tggccggtga caatatcgcg tcccggagcg
     3061 agaacacccc ttggtacgac ggccccactc tgatggagca tctggagacg gtcgagctcg
     3121 acgccgaccg actgcagacc aggccgttcc gcatgcccgt tcagtgggtc aaccgtccca
     3181 acctcgactt tcgcggcttc tccggcctta tcgcatcggg cgtggtcaaa cccggtgatg
     3241 cggtccgcgt cgaaccctcg ggtcgcacct cgaccatcgc ccggatcgtg accaaggatg
     3301 gcgatctgga cgaggccgtc gccggccagt cggtaacgct gaccctgacc gatgaggtgg
     3361 attgctcgcg cggagacatc ctcgcctcag ccacctcagc gcccgaggtc gccgaccagt
     3421 tcgagaccac catcgtttgg atggccgacg aaccgctgct gcccggccgc ccctactggc
     3481 tcaagatcgg cgcccgcacc gtctcggccc agatcaccga gatcaagcac gagatcaacg
     3541 tcaacacgct tgaggagcga gccgccaagt ccttgccgtt gaacggcatc gcggtctgca
     3601 acctgtcgct cgaccgcccc atcggcttcg atgcctattc cgagatcccg gacaccggcg
     3661 gcttcatcct gatcgaccgg atgaccaacg cgacggtcgg cgcagggatg atccgcttcg
     3721 ccctgcgacg ctcccagaac atccattggc aggcgctcga cgtcaccaag gccacccgcg
     3781 ccagcgatct gggtcagaag cctgcggtag tctggttcac tggcctctcc ggcgctggca
     3841 aatcgaccat tgccaatctg gtcgagaaga agctgcggtt cgaaggcaag cacacctatc
     3901 tgctggacgg cgacaatgtc cgtcacggct tgaaccgcga cctcggtttc actgacgccg
     3961 accgggtcga gaacatccgt cgcgtgggcg aggtggcgaa actgatgctc gacgccggcc
     4021 tgatcgtcct gaccgccttc atctcaccgt tccgttccga gcggcggctg gcgcgtgaac
     4081 ttctgggacc gggcgagttc ttcgaggtcc acgtcgatac gcccctggcg atcgccgagg
     4141 cccgcgacgt caaaggcctc tacgccaagg cacggtctgg agcgctgaag aatttcaccg
     4201 gcgtcgacag cccgtatgaa gcgccggaaa accccgagat cgttgtcgat accgcctcga
     4261 tgacggctga ggacgccgcc gagatgatcg tcgaacggct gcgcagcggc ggttaccttc
     4321 aggccgagcc ccagttttcc gagggcggcg gggtctagaa cgccgccaag aaaaaaggcc
     4381 gcgtcatgcg acgcggccgg agtttttctt gggaggaaac accctggaag gggtgggaca
     4441 ccgaagcgtc cgaaccccat ctatgcacga tggatcgaag cgcaagcgtt gccgttcccg
     4501 ttgcgttcca tgccagtctg tcgttgactg ccgttccgag tcggagagcg ctgatgaccg
     4561 agaccctctc cgcagcgcag gcccgccgca tcgcccttgc ggcgcagggg ttcggcggag
     4621 catccgtcac cccttcgccc cgcgcctttc ggcagaccgc gcagcgtctg ggcgtgatcc
     4681 agatcgacag cgtcaacgtc gtcacccgca cccactacct gcccgccttt tcgcggctcg
     4741 gcgactatcc gcgcgacctg ctggagaccg aggcctgggg caagaaacgc agcctgttcg
     4801 aatactgggg ccacgagggg tcgctactgc ccgtcgaaag ccagcctctg ttccgctggc
     4861 ggatggcggc agccgaagcc ggtgaaggca tctggggttc gatcgcccgc ttcggccacg
     4921 agaagagcga ctatgtcgat ggcatcctgg ccgaaatcga gaagcggcgc ggcgtcaccg
     4981 gcggggactt cgccgacggt ccgcgaggcg cgcccggctg gtggtcatgg tccgacggca
     5041 aacgtgcgct ggagtggctg ttctggaccg gacgcatcac caccctgacc cggcgcggct
     5101 tcgagcgcgt gtacgacctg cccgaacgtg ccctgccccg cgccgtcatt gacgcgccga
     5161 cgcccacgcc ggaggatgcc caacgtgagc tgatccgtat ctccgcccgg gccatgggtg
     5221 tcgccaccac ctctgacttg cgcgactatt tccgcctgcc gctggccgaa agccgtgccc
     5281 ggatccacga actggtcgag gcgggcgaac tgatcccggt gacggtgcag ggctggaaca
     5341 agcccgcctt tctcgatccc gccgcccgca agccgcgccg tatcgaggcc gccgccctac
     5401 tgtcgccgtt cgacaatctg atctggtttc gcgaacgaac cgaacggctg ttcggagtac
     5461 gtatccggct ggagatctac accccggccc acaagcgcga gcacggctac tacgtcctgc
     5521 cgctgctgca aaacgaggcc atcaccgccc gggtcgatct gaaaagcgac cgcaagggcg
     5581 gcgcccttct ggttcaggcg gcgtggcgtg aacccgatgc cgacgccgag acgccgcgca
     5641 ttctggccga acagctgcga tccatggccg gctggctggg gctggaacgt gtcgaggtcg
     5701 caggtcgggg cgacctcgca gccgccctcg acgaagcgct ttaggcttcg atcaccgtct
     5761 gcacccagtt gtgcggatcg gcgacgcggc cgcgctggat accggtcagt tttgccagca
     5821 tctcggtcgt taccggaccc ggcttgccgt cgccgatggt aaagtcaccg cccggcgcga
     5881 cgaccttgga gatcgccgtc agcaccgccg ccgtaccgca ggcaaaggcc tcgacgacct
     5941 caccggaggc cacgtccgcg cgccattgct caaaggcata cggggcttca cgcaccgtga
     6001 cgcccgcgtc cttcgccagc tgcaggatcg aggcccgggt cacgcccggc aggatggtcc
     6061 cgcccagcgg aggcgtctgc aacgagccgt ccttgaacac gaagaagatg ttcatgccgc
     6121 ccagttcctc gatccacttg ccttctgcgg cgtcgaggaa gacgacctga tcgcagccat
     6181 tggccacggc ctcggcttgg gcgaccagac tggcggcgta gttgccaccg catttggccg
     6241 cgccggtgcc gccgcgcgcg gcgcgggtgt agtgttccga gacgcggatc gtgacgctgt
     6301 tcttgccgcc cttgaaatag ccgccgacgg gcgaggccag cacgacgaac agatattcat
     6361 tggccgcacg cacgccgagg aaggcttcgg tcgcgatcat gaacgggcgc agatacaggc
     6421 tgccctcgcc ctcggggatc cagtcggcgt cgaccttgac gatttcgcgg accgcttcga
     6481 ggaacatatc ttcgggcagt tgcggcatgg ccagtcgctc agccgacgcc tggaaacggc
     6541 gcgcgttctg ctcgggacgg aacagggcca cgcgaccgtc gtcggtcttg taggccttca
     6601 tgccctcgaa gatttcctgc gcgtagtgca gcactgaggc cgccggatcg agcgggatgg
     6661 gcccgcgcgg ctcgaccttg tgcgaatgcc agccctgtcc cgccgtgtag cgaatggtca
     6721 ccatatgatc ggtgaatacg cggccaaagc ccggatcgac catcaggatg gcgcggtcat
     6781 cggcggtgac gggcgacgga tgaggcgtac ggctgaaggc ggacagaaag gacatggtcg
     6841 gtctctcgat cgaaaaggac gcaaggacgg aagacttcag ggctttgaga gcgcgagcgc
     6901 tacaaggacg tgaagatcgg gcgcatggaa tagccgccgg ataccggttt cactagctgg
     6961 ttttcaccgg agcgcgcggg tctttcgacg atccgcacca cgtcgcgccg gcgcaggtta
     7021 cgctctgaag cccccacaat agcgacgctt gggtcaggct ccaatgagcg ccatatcagc
     7081 gtgatgaaga tattcaccat cggctacgag ggcgcgaccc aggcagacgt catcacaagg
     7141 ctgaaggcgg cgggtgtcga taccctgatc gacgtccgcg ccgtggcggc ttcgcgccgg
     7201 ccgggattct cgaaaacgat cctcggcgcc agtcttaacg cggaagcgat cggttaccgg
     7261 cacctgcggg ctctgggcac gccaaagccg ggccgcgacg ccgcccgcaa agggcacgtc
     7321 gccgaaatgc gagccatctt caacgatcac ctgaccgagc ccgcagccca gcttcaactg
     7381 gccgagcttc gcggactcgc cgcgagttca aaggtcgctc tgctgtgttt cgaggccgac
     7441 cacgccggat gccatcgcgc ggtccttgcc gagcgtctag cgacagagga cgggttcgag
     7501 atcgtcaacc tgtgacgaac cgaccagacg cctggctggc cgtaacatca aggccttcct
     7561 cccccgcgta tgaccgccgc cagggaaagc ccggttgcgg gcctgacgtc cactcggaaa
     7621 cgcgactttc ccgcagcggg agcggctggt ttttcatagc cgacgctggt cgaagaccgg
     7681 cctgctgcgc gccttcagaa tatcagcgcc gagaaagtaa gtcggctcca cagcagatgc
     7741 gcgcttgcga gccttagtcg cggtccgggg cgcccagcgg gaagtagttc cgaaagcgcc
     7801 gggcgtcgtc caccgccctg gcaaaggatg gacgccgcag aaggcgcgcc cggtaggcca
     7861 gcagatatcc ataatgttcc ggtatccggt gcgtccagtc ggcatagaac agcgagggag
     7921 ccgcagcgca gtcggccaag ctgaatatat cacccgccgc ccaggtgcgg tcccgcatcc
     7981 gctcgtccag ccaggcgtag ctggtctcca gcagcttgcg cgcctgctcg acgccgtaag
     8041 gatcgcggtt ctcggccggg cgcagcgcgt cgaatacgaa cttgccctgg ggcgtcatga
     8101 cgtagttgtc gaagaagcga tcgagcatcc gcacctcgac agccatatcc ggatcctcgg
     8161 ggatcagctt catcggcccc gggtgatgaa cctggaggta ttcgataacg gtggtggcct
     8221 ccagcaccac gcgatccttt tcgcgcagga tcgggaagcg tttgatgggc cacagcgaag
     8281 cgaactcctc cccgacgccg gggtcctcca tcatccggta agtgaacgga atatcgtttt
     8341 cgtagaaggc gatgagcacc ttctggcagt aggacgagaa cggatgggcg aacagttcga
     8401 tggtcatgcg acgacccccg cttcggtgtc gtcgaacagg gcgacgaggt tgtcgatccc
     8461 ccaggccgtg ccatgttcgc gaccggccac cgcctcggcc ccgccgacaa gataggagcc
     8521 ttgctctgtg tggatcagcc gggtccggtc gtcctcggct ttgaactcca aggtctgcag
     8581 cgagaccgac aacttatcgc cgttgtggaa catgtcgtag acgaggatga tccgctcgtt
     8641 ctccacgatg tcgtgataaa cggcctgaaa gtccgagagc cggccgtcgg gccaccgcgc
     8701 gtggaaccgc tcccggccgc cgacgcggaa gtcgaaatcg cggtcgaggg tttcgacgtc
     8761 catcggggcc ttgaaccagg cgcccttggc ctcgacactc gtataggcct cgaatacccg
     8821 cgcaggcggg gcgttcagca cgcgctggat ggtaaatgtg gagttggtga cggtcattgg
     8881 tccttctcct cggaagcctt ggccagatag gcctccagct gatcgtattg tcggttccag
     8941 aactgcctgc gctcggcgat ccagtgttcg actcgggtca tggtctcgat ctccagtgcg
     9001 caggtgcgca cgcgaccgat cttctcgctc ttcaccagcc cggcctcctc cagaaccttc
     9061 aggtgctgga ccacggccga caacgtcatc ggcagaggtt cggccagttc gctgaccgag
     9121 gccgggccaa agctcaggcg ttcgaccatg cggcggcgtc cggggtcgga cagggcctgg
     9181 aaggcaagat caagcggctg gctttcatac tgtagcattg acttaagtgt tgagcgatca
     9241 gcaacgagag tcaagcgctg cggaaggtgc aggccaagat gcgaaacgtc cgctcagccg
     9301 tggcaatggc cgtgcccgca gcggatctcg acaaggacgc agatgtggca aaagggccgg
     9361 catcggtcag cgccacgcgc cggcggcgct ggtcattctc gtccacgacc accgtcaccg
     9421 acgcggctcg ctccagcggt tttagatccg ctttcaccgt cgagccgtcc atcgccagca
     9481 gctcggccag agcaccgatc gttggaggtt ccggccgttt cagaccgttc atcagggaga
     9541 actgcccgct tgggatgccg tagggccgac acgcgtcgaa ccgccgcgac agcgcgcggg
     9601 ccgcgcacgt aggtggaagc gtcagcttac ctgccagccg ggctcgcgta gaggcaagtc
     9661 tcgcggctga tcgcgccctc cgggaccgcc agctgcgaga gttcggcgag accgcgcttg
     9721 caggccaact atccccctgc taaaactgca ccggatggtt cagtattctt catctcagct
     9781 aacagacgcc ttcagcgccc tcgcggacgc gacccgtcgg ggcgtgctgg agcagcttgg
     9841 acgcacggat gcctccatca cggagttggc tgacaagttt cacatgacgc tcaccggcat
     9901 gaaaaagcac gtcagcattc tggagcaggc cggtttcgtc acgacggaga aagtcgggcg
     9961 ggtgcgcacc tgccgcctcg gactgcgccg gctcgaggaa gaagcctcct ggatcgaaac
    10021 ctatcgccag ctctggacag cgcgcttcga ggcgctggat caggtggtcg aagatctcaa
    10081 actcaaggaa acagacaatg cctgacccca cccggatcga ccggacctcg gaccgcgagt
    10141 tcgtggtcac ccgtgtgttc gacgcgccgc cccgcatcgt gttccaagcc tggacgacgc
    10201 ccgaactgat gaagcaatgg tgggcgccca ggtcgatggg cgtgccgctt ctcgcttgcg
    10261 aaatggatgt ccggaccgga ggcagctacc ggttcgtgtt cggacatgat gcctcggagt
    10321 cgatggcctt cgtcggccag tacctcgagg tggttccgcc cttgcggctg gtctggacca
    10381 atgaggaaga acaggatggg gctgtgacca cggtgacctt tgaggaacgg aacggcagga
    10441 cccacctcac cgtccatgaa ctctatcctt caaaggttgc cctcgaggcc aaccgcgggg
    10501 cggaagaggg catgccggaa cagttcggac aactcgacgc gctgctgtcg acgcttgatc
    10561 cagcggcctg atcatatgca gtcggcgcat tgtatctggc gagcgagtac agcgatcacg
    10621 tctgcggcag aaggggcccg aaaatagaga gtttagatgg ggcaagcttt tcgcccgcgc
    10681 tacctcagcc ttccagccgg tagccaatcc cggcctcggt cagcacgatg gtgggcatgg
    10741 cgggatcggg ttcgatctta tgtcgcagtt ggccgatgac gacccgcagg tactggacat
    10801 catctgcatg ggccgtgccc cagatggcgg tcaggagatc agtatggctg acgaccttgc
    10861 cggcgcttcg ggccagatag gccaggacgt cgtattcctt gggacggagc ttgaggcgtt
    10921 cttcgccctt catgacgatc cggcggtcga ggtcgatgga gacgtcgccg gcggtgatca
    10981 cgcctgtcgc cacgggacgg agcgcgccct gtctcaagcc tgcccgcagt cgggccagca
    11041 gttcgccgac gccgaagggc ttctcgacat agtcattcgc gccgaggtcg agggccgcga
    11101 tcttctcggc ctccttgtcg cgcgccgaca ggataatgat cggaccggcg tagaactccc
    11161 gggctcgacg cagcacttcc ttgccgtcca tatccgggag gccgaggtca agaaccaccg
    11221 cgtcgggatt ccacagggcg atgccgcgca ggccctcctg cccgttgtcg gcgcgcttgg
    11281 cgtcgtaacc ggcggcgtcg agggcgggcg agaggaagcg gtgaatctgg ggctcgtcgt
    11341 cgatgacgag gatttgcggg cggacggcgg acatcagagc agctcgcgat gggtgacgat
    11401 ggccttgggc aggctgatca gaatacgcgt acccggcttc tcgccattcg tcccttcgtg
    11461 gatcgggctg gcggcggcga tgcggccgtc catggcctcc acgaaaccct tggcgatggc
    11521 gaggcccaga ccgacgccct tggtccggtc gctggactct tccatgcgcc ggaacttgtc
    11581 gaacacccgt tcgagttcgg cggtggggat gcccttgccc tcgtcctcga tgctgatgac
    11641 gatggagttg cgatcctcgt aggcggcgat ctcgatggtc gaaccgtccg ggctgtaggc
    11701 gatggcgttt tccagaacgt tgaccagcgc ctgctcgagc agaccttcgt cgaccttcac
    11761 taggctcaga tcgcctggga aatcgcgtgt catcttgcgt tggccgaggc ggcgcgaaac
    11821 ccgctcggcc gccgcgttga ggatgtcgcg gacgtcggtc cattcggcgc gaaccttcag
    11881 ggcgccgccc tccagccggg tcatgtcgag cagatcgccg acatagcggt tcaaccgttc
    11941 agcctcttcg cggatgctga gcaacaggtc ggcgcgtacc tccggcgtga ggttctttcc
    12001 gtagtcgatc agcgtcgtcg cggagcccag cacggtggac agcggcgtcc tcaggtcatg
    12061 gctgaccgag ttcatcagca cggcccggaa ccggtcggcc cggcgcaagg tctcggtctc
    12121 gacggcgtga cccgcaagct cggcgcgctc gatggcgacg gcgccctgat cgagcagggc
    12181 cagcgccaga cgttcttcat cggaaccggg cgccagcgct tcggcctcga tgccggcgac
    12241 gcccgaacgg ccccgcacgc cctgcagcgg cctgaaggtc cagccggtct gcggcagggt
    12301 gcctgtgcca tgacctgcgg cttctccgtg ctcccaggcc cagcgcgcgg cggccatgtc
    12361 cgacgcgccg agaacgggcg tttcgggagc gccggccgca atgaccagat cgtcgtcctg
    12421 aggcaggagg accaccgtct tggcgccggc ggcttcggat gcctgttccg ccagcacgcg
    12481 cgccgtcgct tgccggtcgt cggcgccggc gagggcctgg ctggctccga gcaaggctga
    12541 aacggcggcg gcccggcgct gggtgcgctt ggcctgttcc ctgacacgtc cggccaatgc
    12601 gccggtgagc agcgccacgc cccagaagac gatcaacgtc aggacgtcgg tcggagagcc
    12661 gatggtgaag ctgtagcgcg gctgcaggaa caggaagttg tagatcagaa aggccgcggt
    12721 cgctgctgtc agcgcaggtc ggaggccgta gagagccccc gcagcgacca cagccgcgag
    12781 gtagatgacg ccgagatcca cacgctcgaa ggccgtgtcc agaacatagg ccgtggcagt
    12841 cgcgaggaac acaaaggcga gaccgaccac atagccgagc cagcgcatgg gcgggcgcgg
    12901 ctgatggggt gcgggcgcag aggtctcttt ctcgtcagtg atgatgtgga tggcgacgcc
    12961 actcgcggat cgcagcagct cggccgccag tgatcgtccc agaagatcgc gcagacggtt
    13021 ggtcttgccc ttgccaagca cgatctgggt gacgtgattg cgggagacgt ggtccatgac
    13081 agcccgaacg acgtcgtcgc cattcagggt cacggtgcgc gcgccgagct gctcggccag
    13141 cttcaaggcg tcgctgagac gggcggcaga ctcgccggta atcgcgccac gatcaacggt
    13201 cgccaccgtc cacggcgcgt ccatcatcat gtcggacagg cgtcgacccg cgcgaaccag
    13261 tgacgccgcc gtgccgtcgc cgccgatgag aacgagaatg cgggcgccgg ccgcccaggg
    13321 gcccggcacc cccttctcgc gcagatggac cagaagctga tcatcgaccg tctgggctgc
    13381 ccgtcgcagg gccagttcgc ggagcgccgt caggttctcg atcttgaaga aattctcgga
    13441 cgccagacgg gcggtctcgg ggacatagac cttgccctcc tcgagccgct tgcgcagttc
    13501 gtcgggcgtg atgtcgacga cctcgatgtc gtcggccttg gacagggcgc tgtccggcac
    13561 cgtctcgcgc tggcgtgcac ccgtgatgcg caacacgacg tctgacaggc tctcgaggtg
    13621 ctggacgttc agcgtggtcc agacgtcgat cccggccttg aggatctcct cgacgtcctg
    13681 ccagcgtttg ggatggcgtg atccggggac gttcgagtgg gcgtattcat cgacgagcag
    13741 caggccgggc ttgcgcgcga tggctgcgtc gatgtcgaac tccatcaggg cgcggccctt
    13801 gtagtcgatg gggttgcggg ccagcacgtc gagaccgcgc agcaggctct cggtctcctt
    13861 gcggccgtgg ctctcgacga ccccgaccag cacgtcgccg ccctcggcct ttcggcggcg
    13921 ggccgagcga agcatctcgt aggtcttgcc gacgccgggc gacatgccga gaaagacctt
    13981 cagccgaccc cgcttgcgcg agaccggccg tagatcctca ggtccggccc cgtccacagg
    14041 cgtcagctcg gccaagtcag gcggccgggg caggcatcag cgcgtcgagc gcacggttgg
    14101 tcatcagcac attgacgcgc ggttgaccga tgaagccgag caaagcgccc gtcgtgttgt
    14161 tgtcgatcac gccctgcacc tgtgccgccg tcaaaccccg ggctgtcgca acgcgcgaag
    14221 cctgaagacg ggcataggcc ggtgagatgt ccgggtcgag gcccgaaccc gaggccgtca
    14281 cggcgtccgc cgggatgacg gtcgcgccgg tatcggcccg gatggcgtcg gcgtcggtct
    14341 tgacgcgttc ggccagatcc ggattcagtg gacccaggtt cgagcccgac gatccggcgg
    14401 cgttatagcc gtcgccggcc gccgagggac gggggtgcag atagcgcgct tcggcgaatg
    14461 gctgggcgat cagggacgag ccctggatcg cgccatctgc gctgcgaacg agactgccat
    14521 tggcctgtgc ggggaagaca ccctgcgcca ggcccgtaac ggccagcgga taggccagcc
    14581 cgaggaccag cgtgaacagg ccggtcatga cgagggcggg acggagttgg gaaagcatgg
    14641 cggcggctcc ttagaatgtc gccttcagcg aggccacggc gcggctgccg tagatgtcgc
    14701 cgaagtcatg ttcgtcggtg tcgtgatagc ggacatcgac agcgagattg gacgtcagct
    14761 gataggccgc gcccacgttc caggtcgtgt aatcgaaggc cgacgaaacg aactggtggc
    14821 cgaccgcgcc ggaaagcgtc cacttgtcgg cagggctgat ggcggcgttg gcttcgatat
    14881 aggtcgcctc gtcttcggac gcgccgaaga agtctggcga gtagtagacg gcggcgccga
    14941 gggtggcggg accgaaagca cgcgatgctg cggccttgaa ctcgacgtag tcgtaatcgg
    15001 cgccgtccgc ctgacccgtg tagaggtagc cgatcacgcc gaggtcgagg ttgaagcctg
    15061 cgaactccgg ccggatcccg ccgtagagat cgatctcggc gttggtgtcg tcatcgcccg
    15121 ggaaggacac gttcgaagcc caggtcccgg catagaagga gccggaggtg acatcgatgc
    15181 cgcccgagat ggccggctct tcttccgtct ggctcacgcc gcggaagacg tagtcggagc
    15241 cgacggcgac gttgaacgcg aacttgggcg cataggaatc agcgatcgac tgcgaggagt
    15301 tctgggcagt gtcagcgtcg gcggtctggg cggcggcttg cgacgcgacg cctgtggcta
    15361 ctgcaaaaag cgcgatcgct gcgacggtgg agttgagttg gatacgcatg actattcctt
    15421 gatttgttcg gccggaacgg gtccgggcca tttgggcgca ttggtttggt cggcattggc
    15481 cagcaggccg acgatgacga tggcggcgat gacgaagatg gcgaaccaga cgtcgttctt
    15541 gccggcggcc ttgaaccgcg atttccggga catgactgtc atcctcacgc gaggccgagg
    15601 ccggtgatga tcaggtcgat ggccttgatg cccacgaagg gcgcgaccag accgccgagg
    15661 ccgtagatca gcaggttgcg ggcaagcagc ttgccggcgc cgacggcgcg gtatttgacg
    15721 ccacgcagag ccagcgggat gagggcgacg atcacgaggg cgttgaagat caccgccgac
    15781 aggatggcgc tctcagggct gcccagatgc atgacattga gcgcgctcaa ggcaggcagg
    15841 gcgaccacga acatcgccgg aatgatggcg aaatacttgg ccacgtcgtt ggcgatcgag
    15901 aaggtggtca aggcgccgcg cgtgatcagc agttgcttgc cgacctcgac gatctcgatg
    15961 accttggtcg ggtcgctgtc gagatcgacc atgttcccgg cttcgcgagc agcttgagcg
    16021 ccggtctgca tggccacgcc gacgtcggct tgagccagcg cgggggcatc gttggcgccg
    16081 tcgccgcaca tggcgaccag acggcccttg gcctgttcgg ccttgatgag ccgcatcttg
    16141 tcctcgggcg tcgcctcggc gaggtagtcg tcgaccccgg cttccgaagc gatggccgcc
    16201 gccgtgaccg ggttgtcgcc ggtgatcatc acggtgcgca gacccatggc gcggagttcg
    16261 gcgaagcggg ccttcacgcc gggtttgacg acgtccttga ggtggatgac gccgaccaga
    16321 cggccgttct cggtgaccgc cagaggcgtg ccgccggagc gggcaatccg gtccacggct
    16381 gcggtgaact cgggcggggc cgaggtgtcc gaaccgagcg acttgagcac ggcgtcaacc
    16441 gcgcccttgc gccaagagtc cttgcccgcg tccagacccg actggcgggt ggtcgctgag
    16501 aaggcgatgg cggtagagcc tgcgggctgt tcgacaacga tcccggcgtt gcggccaagt
    16561 tcgacgatcg agcgaccttc cggagtctca tcagcgagcg aggctgtgac ggcggcgcgc
    16621 agggcgactt caggacgaac gccggcgacg gggatgacct cggtggccat acggttgccg
    16681 aaggtgatgg tgccggtctt gtcgagcaga agggtgtcga cgtcgcctgc cgcctccacc
    16741 gcacggcccg aggtggccag aacgttgacc ttcagcaacc ggtccatgcc ggcgatgccg
    16801 acggcggaca acaaaccccc gatggtcgtg gggatcaggg tgatgaagag ggcgccgagc
    16861 acgatcggat caaggttgac gcccgaatag gcgccgaggc ccagcagcgt cacgaccgcg
    16921 atcaggaaga ccaaagtcag accggccagc aggacggaaa gcgccagttc gttcggcgtc
    16981 ttgcgacggt tcgcgccttc gaccatcgcg atcatgcggt cgaggaaggt cgagcccgga
    17041 gccgaggtga tgcggatctt gagccagtcg gacaccacgg tggtgccgcc ggtgaccgct
    17101 gacctgtcgc cgccgctttc acggatgacg ggggcgctct caccggtgat ggcggcctcg
    17161 ttgaccgagg cgacgccttc gatgatctcc ccgtcggatg ggatgacatc gccggcctcg
    17221 accagaacga tggtgcccac cgtcaggtcg cccgccggca tttgggagac cgtgcccgaa
    17281 gcgggatcga tcaccttggc cggggtggtg acgcgggtcg cccgcaggct gtccgccgcc
    17341 gccttgcctc gtccttcggc gacgctttcg gcgacgttgg cgaacaggac cgtagcccag
    17401 agccacaggg ccagctggat ggcgaagcca gccgcccctc cttgacttgg ccctgaagcg
    17461 atagcgcctg ccgccgagac cgtcgccagc agggcgacga tccaggtgac gaagatgacc
    17521 gggttcttga ccagatggcg cgggttcagc ttgacgaaag cgtcgcccag agcccggccc
    17581 atcatggcgc ccgagaagcc gccggccagc gtgggctgac gccctgtggc cgtttcgatg
    17641 ttgggattag cgtgtgtcat gtgatgtccg ttcggatcac gagccggtca cgccgttgag
    17701 catctggaag tgctcaacga tgggtccgag ggcgagggcg gggaagaact gcagaccgcc
    17761 gaggatgatg atgatgccga tgaccagacc cgcgaactga aggccgtcgg tcggcagggt
    17821 gccggggctc ggcgccagct tgggcttgac gaccagcgaa ccggcgatcg cgaggacggc
    17881 gatggcgggt atgaaccgtc ccatcagcat gccgatgccg agcgtcgtgt tccaccaggg
    17941 cgcgtttgcg gtcagacctg cgaaggccga gccgttgttc gctgcggcgg aagtgtacgc
    18001 gtagagcatc tccgacaggc cgtgcggccc atggttcagc aagccttcca tcgccgtcgg
    18061 gaacaccgcg gagatggccg tgaagccgag gatggccagc ggcaggacca gcacggcgat
    18121 catcgtgtac tggatctcgc gcgcctcgat cttcttgccg aggtattcag gcgtgcgccc
    18181 gaccatcagc ccggcgacga acaccgacag catggccatc aggaccatga tggcgatgcc
    18241 cgatccgacg ccgcccggca ggatctcacc gagttgcatc aggaacatct gaagcccgcc
    18301 acccagcggc atatagctgc cgtgcatgga gttgaccgag ccgctggagg cccctgtcgt
    18361 gagcacggac catacggtcg aggatggcgc gccgagacga acctccttgc cttccatatt
    18421 gaccgaggcg tcgacgcctg cggccaccag agacggcgcc ggctgggtct cgatgaagta
    18481 catggcgccg gccg
//
LOCUS       DASVTM010000233         7504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_446650,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000233 DASVTM010000000
VERSION     DASVTM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446650"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3761
                     /locus_tag="VF633_06370"
     CDS             <1..3761
                     /locus_tag="VF633_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX8470718.1"
                     /translation="ALATAPLANAQAARADGLLFHLSADSSLSADFAAGQAGPTFADK
                     LAMKPTGGADGGYIEGTDDQALAYRAAGNMFAAKGTVAFDWRSRYPVSTTPFVLFRVG
                     YADHSSWDMAFLRIDWNGHGFDAFVTDNNLARVRVSYELPQTPSPDAWMHLAFSWDET
                     DGVVLYVNGKRVAATRTTAVLDAALDQFGIASRIVAPIQVHSRYSFTRGSDYDDLRVY
                     DHALSDAEAAGIAAPKAAFASAPALPASTDDWRAAWNRRFGFDQGSAPYLADPVTRIR
                     RVEFADQRDIAQRMWKGNDGIRETTWPGVYNRSRLPGRNDYFTLPDWNVYSMGGRSAT
                     WFLPDEPWNQIEIQGAAHGRLSAVAARIDASRVIDDTSPQTLAHRPRGAERTTTRVAA
                     HDGGAVRFVNTDQETPIQEIGVYNVVPGAIPESIGDYSYTVRAGVEPSYVATDKLSAF
                     IAGRYPVEERDTLTALPASAPTAPRATPLSGDRPLAHVLIPIDFRRGRAGGPLIRNVP
                     TLSFQEAGGLDGIVLTVPALRSTPGADGLISLNIRVKDPLWPLRDLLDVNVAVKPGEA
                     RTVFLDTRDRILDDDDSLYFTVAASSPDFHASDLDGMGVRMIFKTREAARAEHVADRL
                     AQIKDSYGFLVEERQITRDLGLFDQFYLDLTDLLRVDPENVEGRAYWSEWNTSQPLPA
                     FTQPTPPAGVPLWAFRQIEDLKLVRHFINWWIDNRQVEGEFGGGISDDTDLTNQWVGP
                     ALMGVDPDKLNASLQALIEASYRNGMWTNGFGTILTDELHTYEEGINAISQAALLNRG
                     DPKTLERLMATARRYDELTQVNAAGHRHIISSYYSGSHFARDTPWDWSKAQSYLVLHP
                     GMALVDYNGSPQLKTLLLELADGYLAHGKSATDGAIVWPADINWTTDAQRGSGVSPTN
                     MLLWSAYRWTGDRKYLAPMGEPGSPGWSGINSDVARDLGRPELDQRYVTAAAKTGASS
                     EALTIAAAETGDRSFLERLYAQEIQFGSVRMKMQTEDQWWTDRVELPSDQLQRARLGG
                     VAHMRNRIVPGNRISWRFDDPENAMKVAVLVREPAKDSFRVVVWNMASIPISANIAGG
                     EVTPGEWRVTQGADANGDDRSDAGQTSTIAFGPGQAIPVILPPGASVIEFALAQAGSA
                     MATRPDVGIGAADLVRRGGRLSVTVHSLGAVASPAGQVVIQSPDGAILTTARFGPLAA
                     PNDLQPKTETVSLRMPRSLPAGSEVRLILDGKPSEVSETNNRAALPDR"
     gene            3939..4775
                     /locus_tag="VF633_06375"
     CDS             3939..4775
                     /locus_tag="VF633_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8470719.1"
                     /translation="MAAFQITRDGAVAHLRLTRPEASNALDMSFWRGFAPALRELDRG
                     GEVRALVVSGEGKNFCAGMDISVFSSGAILPAETAAQRQAFHQAVRDLQDALTMLEKV
                     RFPVIAAVHGTCVGAGLDMVSACDIRLASADAYFRIEEINIGMMADVGSLQRLPKLLP
                     EGVVRELAYLGSSLSADRAERLGFVNAILPDADAVVAAALDMAKTIAAKAPLAISGSK
                     AAITYARDHTVAESLEWVAVMQGSLWNPADILAAIQSRRTKEAADFAPLAFLNPVQGP
                     TD"
     gene            complement(4772..6469)
                     /locus_tag="VF633_06380"
     CDS             complement(4772..6469)
                     /locus_tag="VF633_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8470720.1"
                     /translation="MDRRGFLSLIGAGAATASAAVAEASPQGTVAFLHGVASGDPSTT
                     AAIFWTRVTPADTSNSAIPVVLEIARDAGFTDRVRRSTGLSAEGARDFTVKHDLDGQG
                     LEPGSEYFYRFVASGVTSPVGRVKTLTEGATPEIALAVVSCQLYPGGLFNAYDAIAKL
                     DRVDAVVHLGDYIYEYGAEPDDYGMTTGAALNRAPLPAHEIVTLADYRQRHAQYKTDP
                     DLQAAHARAPFICVWDDHEVANDVWTTGAENHQAATEGDFNARKAAALKAYFEWMPIR
                     EPVTGKMAEAIQRTFHFGDLASLMMVETRLQGRQQQLTYAADITMKAGPDGKPMPDAA
                     AFEAKRNAPERDLLGDAQREWLGRELAASKTAGRPWQVLGNQVVMARVAGPNIAAMAT
                     PDQIAGMLGGLPPAIRVQVQQSAALFALGLPFNLDSWDGYPAGRERLYETFKAAGVQP
                     VVLAGDSHAFWVNDLKNAAGERVAVEFGTSSISSPSPQDSIGNFPLGAALEATNEEVL
                     FCDQSAKGYILLTLTPTEARADLIGMSTIMAKPFAVRTVKTYRIAQTAAGLGPTTAV"
     gene            complement(6477..>7504)
                     /locus_tag="VF633_06385"
     CDS             complement(6477..>7504)
                     /locus_tag="VF633_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470721.1"
                     /translation="QSLAARAAALAAAGNPAGAAAVQAQVVGILGGLANPNAVNLPVA
                     SGLPLFGLTAQPTANNGDFTYGDLEDDGLTWRLTARYALSDDASLYANYARGRRPKVL
                     SPRGPSAPLGAVTFNEIDAETVDSYEIGAKAALMDRRLRVDGAVYMYTYNNFQTTVQS
                     GTQFVVTNAGEAEAYGFEGQVNWALSDQIDLFGTYGYNHARFTTGIYDGNQLRLSPDH
                     RASVGLIWTLPVAGGAFQIQPTYTWQSEVFFDDNNDRTDLQTSAMGNLVPDTIVDELQ
                     ESYGLLNLRVRYTPTGANWSVEAFGDNLLEEEFIKDAGNTGDALGVPTFIAGEPRMYG
                     VNLSLRY"
BASE COUNT         1269 a         2508 c         2435 g         1292 t
ORIGIN      
        1 ctgcgctggc cactgctccg ctcgcgaatg cgcaggctgc ccgagccgat ggcctgctgt
       61 ttcatctcag tgccgactcc tcgctgagcg ccgactttgc cgcgggtcaa gccggaccga
      121 cgttcgccga caaactcgcg atgaagccca caggcggggc tgatggcggc tacatcgaag
      181 gcacggacga tcaggctctg gcctaccgcg ccgctggcaa catgttcgca gccaagggga
      241 ccgtcgcctt cgactggcgg tcacgctatc cggtcagcac gacgccgttc gtgctgttcc
      301 gcgtaggtta cgccgaccac tcgagctggg acatggcctt cctgcggatc gactggaacg
      361 gccacggctt cgacgccttc gtgaccgaca acaacctcgc tcgcgtccgg gtgtcgtacg
      421 aactgccgca gacaccatcg cctgacgcct ggatgcatct tgccttcagc tgggacgaga
      481 cagacggcgt cgttctttac gtcaacggca agcgcgtcgc ggccacaagg accaccgccg
      541 ttctcgacgc agcactggac cagttcggca tcgcctcgcg catcgtcgcc ccaattcagg
      601 tccacagccg ctacagcttc acgcgcggtt ctgactacga tgacctgagg gtctatgacc
      661 acgccctgtc ggacgcagag gccgccggca tcgctgcgcc caaagctgcg tttgcttctg
      721 ctccggccct tcctgcaagc acagatgact ggcgcgccgc ctggaaccga cgcttcggtt
      781 tcgatcaggg ttccgcgcct tatctggcag accctgtcac tcgcattcgg cgcgttgagt
      841 tcgcggacca gcgcgacatt gcccagcgca tgtggaaggg caatgacggt atccgcgaga
      901 cgacctggcc cggcgtctac aaccgctcgc gcctgcccgg caggaacgac tatttcaccc
      961 tgcccgactg gaacgtctat tcgatgggcg gcaggtcggc gacgtggttc ctgccagacg
     1021 aaccctggaa ccagatcgag attcaaggcg ccgcccacgg ccgtctgtcc gccgtcgccg
     1081 cccgcatcga cgccagccgc gtcatcgacg acacctcgcc gcaaacactc gcccaccgcc
     1141 cgcgcggcgc cgaacgtacc acgacccgcg tcgctgccca cgatggcggc gcggtgcgct
     1201 tcgtcaacac ggatcaggaa accccgattc aggagatcgg cgtctacaac gttgtgccgg
     1261 gcgccattcc ggagagcatc ggcgactatt cctacaccgt gcgcgcgggt gtcgagccga
     1321 gctatgtcgc caccgataaa ttgtcggcct tcatcgccgg tcgctatccg gtcgaggaac
     1381 gcgacaccct gaccgccttg cccgccagcg caccgaccgc gccgcgcgct acccccctca
     1441 gcggcgaccg gcccttggcg catgtgctga tcccgatcga cttccgacgg ggccgcgcag
     1501 gcggaccgct catccgcaac gtgccgacgc tgagctttca ggaagccggc ggactggacg
     1561 gcatcgttct aacggttccg gcgctgaggt cgacgccggg cgctgacggc ctgatctcgc
     1621 tcaacattcg cgtcaaggat ccgctctggc ccctgcgcga cctgctggac gtcaatgtcg
     1681 cggtcaagcc gggtgaggcg cgcaccgtct tcctcgacac ccgcgaccgc attctcgatg
     1741 atgacgacag cctgtatttc actgtcgcgg cttcgagtcc cgacttccat gcctccgacc
     1801 tcgatggcat gggcgtgcgg atgatcttca agacccgcga ggccgcccgg gccgagcacg
     1861 tcgccgaccg cctcgcgcag atcaaggaca gctacggctt cctcgtcgag gaacgccaga
     1921 ttacccgcga cctcggcctg ttcgaccagt tctacctgga cctcaccgac ctgctgcggg
     1981 tcgatccgga gaacgtcgag ggtcgcgcct attggagcga atggaacacg tcccagcccc
     2041 tccccgcctt cactcagccc acgccgccgg ccggtgtccc cctgtgggcc ttccgccaga
     2101 tcgaggacct caagctggtc cgccacttca tcaactggtg gatcgacaac cgtcaggtcg
     2161 aaggcgagtt cggcggcggc atctccgacg ataccgacct gaccaaccag tgggtcggcc
     2221 cggcgctgat gggcgtcgat cccgacaagt tgaacgcgtc gctgcaggcc ctgatcgagg
     2281 ccagctatcg caacggcatg tggaccaacg gtttcggcac gatcctgacc gacgaacttc
     2341 acacctacga ggaagggatc aacgccatca gccaggcggc gctgctcaat cgtggcgatc
     2401 cgaaaacact ggaacggctg atggcgacgg cgcgtcgcta cgacgagctg acgcaggtca
     2461 atgcggccgg tcatcgccac atcatctcca gctattacag cggctcccat ttcgcgcgcg
     2521 acacgccgtg ggactggtcc aaggcgcagt cctatctggt gctgcacccg ggcatggcgc
     2581 tggtggatta caacggctcg ccacagctga agaccctgct gctggagctt gcagacggct
     2641 acctggccca cggcaagtcc gcaaccgatg gtgcaatcgt ctggcccgcc gacatcaact
     2701 ggaccaccga cgcccagaga ggctccggag tcagtcccac gaatatgcta ctctggtcag
     2761 cgtatcgttg gaccggcgac cgaaaatacc tcgctccaat gggtgagccc ggctctccgg
     2821 gttggtcggg tatcaacagc gacgtggccc gcgacctcgg ccgccccgaa ctggaccagc
     2881 gctatgtcac ggccgctgca aagacgggtg cgtcttccga agccttaact atcgccgctg
     2941 ccgagacagg cgaccgcagc ttccttgaac gtctttatgc gcaggaaatc cagttcggat
     3001 cggtccgcat gaagatgcag accgaggacc aatggtggac ggatcgtgtc gaactaccgt
     3061 ccgaccagtt gcagcgcgcg cgtctcggcg gtgtcgccca tatgcgcaac cgtatcgtgc
     3121 ccggaaaccg gatcagctgg cgcttcgacg atcccgagaa cgcgatgaag gtcgccgtgc
     3181 tggtgcgcga gccagccaag gacagcttcc gcgtggtggt ctggaacatg gcgtcgatcc
     3241 ccatctcggc caatatcgcc gggggcgagg tcacgcccgg cgaatggcgg gtgacgcagg
     3301 gagccgacgc taatggcgac gatcggtctg atgccggtca gacttcgacg atcgcgtttg
     3361 ggccaggaca agcgatcccg gtgatcctgc cgccgggcgc gagcgtcatc gagtttgcgc
     3421 tggcccaagc cgggtccgcc atggcgacac gtccggacgt cggcatcggc gccgccgatc
     3481 tggtcaggcg cggaggacgg ctgtccgtca ctgtgcatag ccttggcgcg gtcgcatctc
     3541 ctgccggtca agtggtgatc cagagcccgg atggggcgat tctgacgacg gctcgtttcg
     3601 gccctttggc tgctccgaac gatctccaac ccaagacgga aaccgtttcg ctgcgaatgc
     3661 ctcgttcgtt gcctgcgggg tcggaagtgc ggctcatcct cgatggcaag ccctcggaag
     3721 tcagcgagac gaacaaccgg gctgctctgc cggatcgttg atccattgaa cgcaggcgcc
     3781 gtgacgaacg cccaaagccg gcgcgagagg aactgtgcca gcgaaccggt tcccgtttcg
     3841 ggtccctgtg tgctcaatcg ccacccgcct cgacatcaac cttgccgagc ggcggcgatg
     3901 ggatgcatga cggcgcatcg tacagttcgg atactcacat ggccgccttc cagattacac
     3961 gcgacggcgc ggtggcgcac cttcgactga cccgtcccga ggcttcgaac gcgctcgaca
     4021 tgagcttctg gcgcggcttt gccccggccc ttcgtgaact ggatcggggc ggcgaggtcc
     4081 gtgcgctggt ggtctccggc gagggcaaga atttctgcgc cgggatggat atctcggtct
     4141 tctcaagcgg cgcgatcctg ccggcagaga ccgcagcgca gcggcaagct ttccatcagg
     4201 ccgtccgtga cctgcaggat gcgctgacca tgctggagaa ggtgcggttc cccgtcatcg
     4261 cggccgtgca cggcacctgc gtcggtgcgg gtctcgacat ggtctccgcc tgcgacatcc
     4321 ggctggcgtc tgcagacgcg tatttccgga tcgaggaaat caacatcggc atgatggcgg
     4381 acgtcggaag cctccagcgc ctgcccaagc tgctgccgga aggcgtggtg cgggagctgg
     4441 cctacctagg ttcaagcctg agcgccgacc gcgccgaacg gctcggcttc gtcaacgcca
     4501 tcctgcccga tgcagacgca gtcgttgccg ccgcgttgga catggcgaag accatcgcgg
     4561 ccaaggctcc gctggcgata tccggttcga aagccgccat cacctacgcc cgcgaccaca
     4621 ccgtggccga gagtttggaa tgggtagccg tgatgcaagg ctcgctctgg aacccggccg
     4681 acatcctcgc cgcaatccag tcgcgtcgga cgaaggaggc tgcggatttt gccccgctcg
     4741 cgttcctcaa cccggtgcag gggccaaccg actagaccgc cgttgttgga cccaaacccg
     4801 ccgccgtctg tgcgatccga taggtcttca ccgtgcggac ggcgaagggc ttggccatga
     4861 tggtcgacat gccgatcagg tcggcgcgcg cctcggtcgg tgtgagggtc agcaggatgt
     4921 agcccttggc gctctggtcg cagaacagca cttcctcgtt ggtggcttcc agagcggcgc
     4981 cgagcgggaa gttgccgatg gagtcctgag gcgacgggct ggagatcgag ctggtcccga
     5041 actcgaccgc aacccgttca ccagcagcgt tcttcaggtc gttgacccag aacgcgtggc
     5101 tgtcgccggc cagtacgaca ggctgaacgc cggccgcctt gaaggtctcg tacagacgct
     5161 cacgaccggc cggatagccg tcccagctgt cgagattgaa cggcaggccc agggcgaaca
     5221 gcgccgccga ttgctgcacc tgaacgcgga ttgcaggcgg caggccgccc agcattccgg
     5281 cgatctggtc gggcgtcgcc atcgcggcga tgttcggtcc ggcgacgcgc gccatcacca
     5341 cctgattgcc cagcacctgc caaggccggc cagcggtctt ggaggcggcc agttcgcggc
     5401 ccagccactc gcgttgagcg tctccgagca ggtcgcgttc gggtgcgttg cgcttggcct
     5461 cgaaggcggc ggcgtccggc attggcttgc cgtcaggacc ggccttcatc gtgatgtcgg
     5521 ccgcgtaggt cagctgttgc tgacggcctt gcagccgggt ctccaccatc atcaggctgg
     5581 cgaggtcgcc gaagtggaac gtccgctgga tggcctcggc catcttgccg gtgacgggtt
     5641 cgcggatcgg catccactcg aaatacgcct tcagcgccgc cgccttgcgg gcgttgaagt
     5701 caccttcggt cgcggcctga tggttctcgg cgccagtggt ccagacgtcg ttggcgacct
     5761 cgtggtcatc ccagacgcag atgaagggcg cacgcgcatg ggcggcctga aggtcgggat
     5821 cggtcttgta ctgggcgtga cgctggcgat agtcggccag ggtgacgatc tcgtgcgcag
     5881 gcaacggcgc ccggttgagg gctgcgccgg tggtcatgcc gtagtcgtcg ggctcggcgc
     5941 cgtattcgta gatgtagtcg ccgaggtgaa ccacagcgtc gacgcgatcc agcttggcga
     6001 tggcgtcata ggcgttgaac aggccgcccg gatacagctg gcacgagacc accgcgaggg
     6061 cgatctcagg cgtcgcgcct tcggtcaggg tcttgacccg gcccaccggc gaggtgacgc
     6121 ccgacgcgac gaaacgatag aaatattcgc tgccgggctc gagcccctga ccgtccagat
     6181 cgtgcttgac cgtgaagtcg cgcgcccctt ctgcggacag gccagtcgaa cgccggaccc
     6241 tgtcggtgaa gcccgcgtcg cgcgcgatct cgagcaccac cgggattgcg ctgttggacg
     6301 tatccgccgg ggtcacccgg gtccagaaga tcgccgccgt ggtggacgga tcgcctgaag
     6361 cgacaccgtg aaggaaggcg acggtcccct gcggagacgc ctcggcgacg gcggcggagg
     6421 cagtggccgc ccccgccccg atcaggctca gaaagccgcg acggtccatg ctcatcctag
     6481 taccgcaggc tcaggttgac gccgtacatg cgaggctcgc cggcgatgaa ggtcggcacc
     6541 cccagggcgt cgccggtgtt gccggcgtcc ttgatgaact cttcctccag caggttgtcc
     6601 ccaaaggcct cgaccgacca gttggcgccc gtcggcgtgt agcggacccg caggttcagc
     6661 aggccatagc tctcctgcag ctcgtcgacg atcgtatcgg gaaccagatt gcccatggcc
     6721 gaggtctgca agtcggtgcg gtcgttgttg tcgtcgaaga agacttccga ctgccaggta
     6781 taggtcggct ggatctggaa ggcgccgccg gcgaccggca gggtccagat caggccgacc
     6841 gaggcccggt gatccggcga caggcgcaac tggttgccgt catagatgcc ggtggtgaac
     6901 cgcgcgtggt tgtaaccata ggtcccgaac agatcgatct ggtcgctcag cgcccagttg
     6961 acctgaccct cgaaaccata ggcctcggcc tcgccggcgt tggtgacgac gaactgggtc
     7021 ccgctctgaa ccgtcgtctg gaagttgttg taggtgtaca tatagacggc gccgtctacg
     7081 cgcaggcggc gatccatcag ggccgccttg gcgccgatct cgtagctgtc gaccgtctcg
     7141 gcgtcgatct cgttgaaggt cacagcgccc agtggagccg acgggccgcg tgggctcagc
     7201 accttgggac gacgaccgcg agcgtagttg gcgtacaggc tggcgtcgtc cgagagggca
     7261 tagcgcgccg tcagacgcca ggtcaggccg tcgtcctcca gatcgccata ggtgaagtcg
     7321 ccgttgttgg ccgtcggctg ggccgtcagg ccgaacagcg ggagaccgga cgcaacgggc
     7381 aggttgaccg cgttgggatt ggccagcccg cccaggatgc caacgacctg ggcttgcacg
     7441 gcggcggcgc ccgctggatt accggcggcg gcgagggcgg ctgcgcgagc cgccaggctt
     7501 tgga
//
LOCUS       DASVTM010000234         4112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_607774,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000234 DASVTM010000000
VERSION     DASVTM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607774"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..2700
                     /gene="alaS"
                     /locus_tag="VF633_06390"
     CDS             37..2700
                     /gene="alaS"
                     /locus_tag="VF633_06390"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX8470722.1"
                     /translation="MTSLKQIRSTFLDYFAADGHTKVQSAPLVPQNDPTLLFVNAGMV
                     PFKDYFTGASTPPYKRATSSQKCVRAGGKHNDLDNVGYTSRHHTFFEMLGNFSFGDYF
                     KEHAIDRAWNLITKEFGLDPNRLLVTVYIDDDEAFDLWKKIAGIPESRIIRIAGSDNF
                     WSMGDTGPCGPCTEIFWDHGDHIPGGPPGSPDEDGDRFVEIWNNVFMQYEQAADGSRT
                     LLPKPSIDTGMGLERVAAVLQGVHSNYDIDLFKTLIAASEEATGTHATGDQAASHRVI
                     ADHLRSSSFLIADGVTPSNEGRGYVLRRIMRRAMRHAHLLGAADPLMHRLVPTLVSEM
                     GGAFPELNRAQAFIEDTLKQEEIRFRTTLGRGMGLLEDASRDLGEGGVLSGHTAFTLY
                     DTYGFPLDLTQDEARRRGFTVDTDAFDEAMTTQRNRSREHWTGSGQTAATGEWLALRD
                     RLGPTVFTGYDAVEGSGEVLAIVANGASVDELGSGDVAEILLDQTPFYGEGGGQSGDK
                     GEIEWSDASGQQGEGIVLDVIKHAGGDLFAHKVEITSGALSIGARVQLRVTANARLTT
                     RANHSAAHLLHAALAHVLGPHVAQKGQLVDADRMRFDFSHNAPVTEAELAALEDEVNA
                     VIRQNLPAETKLMTPQDAIDAGAIALFGEKYGDEVRVLTLGRSLTDAAKPYSVELCGG
                     THVARTGDIALFKIVSETGVAAGVRRIEALTGEAARQFLLAQAGVAKTLAQTFKVQPL
                     DVPARVEALTTERRALEKQVADLKKQLALGGGSSANAAPEEINGIKLIARVLDGVDGK
                     GLRGVAEDFRKQVGSGVVALIGVTDGKAAVTVAATSDVTDKVNAADLARAAVIAMGGQ
                     GAGGKADFAQGGAPDGSKAEAGLAAIRAALA"
     gene            complement(2878..>4112)
                     /locus_tag="VF633_06395"
     CDS             complement(2878..>4112)
                     /locus_tag="VF633_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX8470723.1"
                     /translation="ADWVNADITVTTDADQTPIAPGYQRSDVTKDGRRTVRFVTEAPV
                     LYFLSVQSARYQIARESHNDVDMVIYYDAQHPRNVPRMMTALTKSLDYYSANFSPYQF
                     RQARISEFPYGSFAQSYPNTFAWSENLGFIADVSNVEKIDYVTYVAAHEFGHQWWAHQ
                     IVGADMQGATALSETLAQYSALMVMEKMYGAEQIRRFLKFELDSYLRGRGTERLEEVP
                     LQRVENQQYIHYRKGAMVMYLMRDQIGEEAVNRTLQALLQRYAFHSAPYARSVDLVAL
                     LRANAPADKQALITDLFEKITLYDLKTTATRAVQRADGKWDVTLTVDAKKLYADGKGV
                     ETPAPLNESIDIGLFTAEPGKKAFDRRSVVVMERRPLHSGVQTFRFVTAVKPTFAGVD
                     PYNKWIDRNSDDNLKAVG"
BASE COUNT          701 a         1432 c         1291 g          688 t
ORIGIN      
        1 cgagacctcg cccaagactc cacccgagag ccgcccatga ccagcctgaa acagatccgt
       61 tcgaccttcc tcgactattt cgcggccgac ggtcacacca aggtccagtc ggctccgctg
      121 gtgccgcaga acgacccgac cctgctgttc gtcaacgcgg gcatggtgcc gttcaaggac
      181 tatttcaccg gggcctccac gccgccgtac aagcgcgcga ccagctccca gaaatgcgtg
      241 cgtgcgggcg gcaagcacaa cgatctggac aacgtcggat atacgtcgcg tcaccacacc
      301 ttcttcgaga tgctgggcaa cttttcgttc ggcgactact tcaaggaaca cgcgatcgac
      361 cgcgcgtgga acctgatcac caaggagttc ggtctcgatc cgaaccgcct gctggtcacc
      421 gtctatatcg acgacgatga ggcgttcgac ctgtggaaga agatcgccgg catccccgag
      481 agccgcatca tccgcatcgc cggatcggat aatttctggt cgatgggaga caccggcccg
      541 tgtggtccct gcaccgagat tttctgggac cacggcgatc atatccccgg cggccctccc
      601 ggttcgcccg acgaagacgg cgaccggttc gtggagatct ggaacaacgt cttcatgcag
      661 tacgagcagg ccgccgacgg cagccgcacc ctgctgccca agccctcgat cgacaccggc
      721 atgggtctgg aacgcgtcgc cgccgtgctt cagggcgtcc attccaacta tgacatcgac
      781 ctgttcaaaa ccctgatcgc ggcctcggaa gaggcgaccg gcacccatgc gaccggtgat
      841 caggccgcca gccaccgtgt cattgccgac cacctgcgct cgtcgtcctt cctgatcgcc
      901 gacggcgtca ctccgtcgaa cgaagggcgg ggctatgtcc tgcgccgcat catgcgccgc
      961 gccatgcgcc atgctcacct gctgggcgcc gccgatccgc tgatgcaccg gctggttccg
     1021 acgctggtgt cggagatggg cggggccttc cccgagctga accgcgcgca ggccttcatt
     1081 gaggacacgc tgaagcagga ggaaattcgt ttccgcacca cgctcggtcg cggcatgggc
     1141 ctgttggaag acgcctcgcg cgatctcggc gagggcggtg ttctgtcggg ccataccgcc
     1201 ttcactctct atgacaccta tggtttcccg ctcgacctga cccaggacga ggcgcgccgt
     1261 cgcggcttca ccgtcgacac cgacgccttc gacgaggcca tgacgacgca acgcaaccgt
     1321 tcgcgcgagc actggaccgg ctctggccag accgctgcga ccggcgaatg gctggccctg
     1381 cgcgaccgcc tcgggccgac cgtcttcacc ggctatgatg ccgtcgaagg ctcgggggaa
     1441 gttttggcca tcgtcgccaa cggcgcctcg gttgacgagc tcggctcggg cgatgtcgct
     1501 gaaatcctgc tcgaccagac gcccttctac ggcgaaggcg gcggccagtc tggcgacaag
     1561 ggcgagatcg aatggtccga cgcctcgggc cagcaaggcg aaggtatcgt gctcgacgtc
     1621 atcaaacacg ctggcggcga cctgttcgcg cacaaggtcg agatcacatc cggcgccctg
     1681 tccatcggcg cgcgcgtcca gttgcgggtc acggccaacg cccgcctgac cacccgcgcc
     1741 aaccactccg ccgcccacct gctgcacgcc gcgctggcgc atgtgctcgg cccgcacgtg
     1801 gcccagaagg gccagttggt cgacgccgac cgcatgcgtt tcgacttcag ccacaacgcc
     1861 ccggtcaccg aggccgaact cgccgcgctg gaggatgagg tcaacgccgt gatccgtcag
     1921 aaccttccgg ccgagaccaa gctgatgacg ccgcaagacg ccatcgacgc cggcgccatc
     1981 gccctgttcg gcgagaaata cggcgacgag gtccgcgtcc tgacgctcgg ccgctcgctg
     2041 accgacgccg ccaaacccta ttcagtcgaa ctgtgcggcg gcacccacgt ggcccgcacc
     2101 ggcgacatcg ccctgttcaa gatcgtgtcc gaaaccggcg tcgccgccgg ggtccgccgc
     2161 atcgaggccc tgaccggcga agccgcgcgc cagttcctgc tggcccaggc cggggtggcc
     2221 aagacgctgg cccagacctt caaggtccag ccgctggatg tccctgcccg cgtcgaggcc
     2281 ctgaccaccg aacgccgcgc gctcgagaaa caggtcgccg acctgaagaa gcaactcgct
     2341 ctcggcggcg gctcgtccgc caatgctgcg cctgaagaga tcaatggcat caagctgatc
     2401 gcccgcgttc tcgacggcgt cgacggcaag ggcctgcgcg gcgtggccga ggacttcagg
     2461 aaacaggtcg gctccggcgt cgtcgccctg atcggggtca ccgacggcaa ggcggcggtc
     2521 accgtcgccg cgacctcgga tgtcaccgac aaggtcaacg ccgccgacct cgcccgcgca
     2581 gccgttatcg cgatgggcgg ccagggcgcc ggcggcaagg ccgacttcgc ccagggcggc
     2641 gcgccggacg ggtcgaaggc cgaagccggt ctggccgcga tccgggcggc gctggcttaa
     2701 caccctctcc cggcgggaga gggcttgagg ctcgcagcgc agcgatgcgt ttcagccgaa
     2761 agggtgaggg gctggcgcta accgttgagg gtgacccctc accctttcgc gccaggacga
     2821 cggcaatcgc cgccgtgcgc tcaagccctc tcccgccggg agagggtacg tccgccccta
     2881 ccccaccgcc ttcagattat cgtccgagtt gcggtcgatc catttgttgt acggatccac
     2941 cccggcgaac gtcggcttga ccgccgtgac gaacctgaac gtctgcacgc ccgagtgcag
     3001 cggacggcgc tccatcacca ccaccgaccg gcgatcgaac gccttcttgc ccggttcggc
     3061 ggtgaacagg ccgatgtcga tgctctcgtt cagcggcgcg ggcgtctcga cacccttgcc
     3121 gtcggcgtag agcttcttgg cgtccaccgt gagcgtcaca tcccatttgc cgtcggcgcg
     3181 ctggaccgcg cgggtcgccg tcgtcttcag gtcatagagg gtgatcttct cgaacaggtc
     3241 ggtgatcagc gcctgtttgt ccgccggagc attggcccgc agcagcgcca ccagatccac
     3301 cgaccgcgca tagggcgcgc tgtggaaggc atagcgttgc agcagcgcct gcagggttcg
     3361 gttcacggcc tcttcgccga tctggtcacg catcaggtac atgaccatcg cacccttgcg
     3421 gtagtgaatg tactgctggt tctcgacgcg ctgcagcggg acctcctcca gccgctccgt
     3481 gccccgacca cgcaggtagg agtcgagttc gaatttgagg aagcggcgga tctgctccgc
     3541 cccgtacatc ttctccatca ccatcagcgc cgaatactgc gccaaggtct cggagagcgc
     3601 cgtcgccccc tgcatgtccg caccgacgat ctggtgcgcc caccactggt gtccgaactc
     3661 gtgcgcggcg acataggtga cgtagtcgat cttctcgacg ttcgacacgt cggcgatgaa
     3721 gccgaggttt tcgctccagg cgaaggtgtt cggataggac tgggcgaacg agccgtacgg
     3781 gaattcgctg atccgggcct gacggaactg gtacggcgag aaattggccg agtagtagtc
     3841 cagcgacttc gtcagcgccg tcatcatccg gggcacgttc cggggatgct gggcatcgta
     3901 gtagatgacc atgtcgacgt cgttgtggga ctcacgcgcg atctgatagc gggccgactg
     3961 caccgacagg aagtacagca ccggcgcctc ggtcacgaac cgcaccgtgc gacgcccgtc
     4021 cttcgttacg tccgagcgtt gatagccggg cgcgatcggg gtctggtcgg cgtcggtcgt
     4081 caccgtgatg tcggcgttga cccagtctgc cc
//
LOCUS       DASVTM010000235        12545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_978933,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000235 DASVTM010000000
VERSION     DASVTM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978933"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF633_06400"
     CDS             complement(<1..294)
                     /locus_tag="VF633_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470724.1"
                     /translation="MGLSAITLDNDDQEHSVPESLHPVFHKVIGAFMDGDFRLLEHEI
                     EGVGRIDAEIAKAIAENILAYGDRLAFLDTSVWERSCYRWMGGYWQLLIDLSTE"
     gene            complement(317..1240)
                     /gene="prmC"
                     /locus_tag="VF633_06405"
     CDS             complement(317..1240)
                     /gene="prmC"
                     /locus_tag="VF633_06405"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX8470725.1"
                     /translation="MNDTTSSPAPATPPSSGPTLVSAWKSAQAQLKSARIDSPSIDAR
                     LLLEAASGASRMDILTDPYRLLTDDQQAVLDGYVERRLKREPVSRILGRKGFWKIMLN
                     VTPDVLSPRPDTETILDIVMLAHARNETFNVIDLGTGSGAILLAILSERPAAKGVGTD
                     ISSEALAVARENAANLDLDGRATFLRTEWAAGFGDDSFDLVVSNPPYIPSGDIPGLDP
                     EVREHDPLLALDGGPDGLEAYRELAPEIMRILKPGGVFAVEIGWDQGPQVKALFEAAG
                     FKDVIVVKDLGDRHRVVTNGPDPRTNPIPTS"
     gene            complement(1237..1995)
                     /locus_tag="VF633_06410"
     CDS             complement(1237..1995)
                     /locus_tag="VF633_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX8470726.1"
                     /translation="MTVVAALYRHPVKGFTPEPLTQAILTTDGWFPGDRMWAMENGPS
                     GYDPGSPEQIPKQAFTVLMRQAMLARVRTCWDEATNILTAEIDDQRLCVRLDEPDDGA
                     AFATVLADFLGEEALGPLRLIAAGPSHRFMDSSKGRVSILNLNSVRDLERRLGQPLDP
                     LRFRANIWIEGLEPWVENDWSDQSISIGETVLRGAKPTVRCAAIDVDPATGVRDLEVV
                     RALFDNYGHVFCGLYVSIETGGRIVVGDPVARPA"
     gene            2095..3357
                     /locus_tag="VF633_06415"
     CDS             2095..3357
                     /locus_tag="VF633_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8470727.1"
                     /translation="MAFSPKTPFKRPVGSRGSIWRFVGAAVGGLATGAGAAHLLYTQG
                     HKVGIELRPRRPEPLTPKAPTPEDYDARDPGRGRLAQRPEHIPHKGWIDILWRVGAAY
                     FGDRVGFVAGGVTFFLVLSLFPTLAAFVTLYGLFADPADAWSRLQFLYSILPSNVAEF
                     LGEQMQRLALNPSGQLTFTLVWTLLLSLWAANGGVKTLFYGLNVAYHEVEKRNLVRYN
                     LICMAFTLSGLTAVLVTAGLVVGAPIVLAVFGLNEEFGALAWLRWPLLLVGYIGALTL
                     IYRFGPCRQKARWRWLTPGAVFAATLSVTVSFLFSWYLSHFVRLDSYGPLAAFMGFLL
                     WIWISVQIILMGAEMNAEIEHQTALDTTTGAARPIGERGALVADTVGPRRGSPAALAF
                     TLKHAEAVADRVVKRRAKTAVVKPVEEP"
     gene            complement(3367..5463)
                     /locus_tag="VF633_06420"
     CDS             complement(3367..5463)
                     /locus_tag="VF633_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX8470728.1"
                     /translation="MAGQGYGGRQGSQGGAGAKPQRSALQKFFYWGAVLAVWGVIFLV
                     VFFAVFARGLPDTSSLYNVDRQPSITYLDRNGALIATRGTQQAPPADLDSLPDYVPAA
                     FIAIEDRRFWYHPGFDPIGMSRAMVRNMRAGRVVQGGSTLTQQLAKNLFLTPDQNMKR
                     KVQELMLAVWLELKFTKKEILALYLNRVYFGAGAYGIEAASQRYFDKSAKDLTVGEAA
                     LLAGLLKAPSRYSPVSESKRAATRATVVLDQMVETGVITSAQRDQAVQEPVRVSRTLA
                     SQHAQYFIDWLDKQIRGLVGEPTEDMVVETTLDLTIQTSAERAVNRILTRDASKGVQQ
                     AALVALDGDGRVRAMIGGASYADSQFNRAVDAQRQAGSSWKPFVYLAAMEAGYTPQTP
                     VVDEPVTIGTWSPKNYGGTYGGPTTIAHAVAHSTNTIAASVADKVGRDSVSRVARRLG
                     ITSRISLDPAMALGTSDVSPIEMATAYDAFANGGRRVEAYGISRIRTPQGRVIYQHGN
                     SGAIGQQTINNPPLYYMNQMLRGVMTGGTGTSAAIAGRDLAGKTGTTSDYKDAWFVGY
                     TGGFVAAVWVGKDDATPMRGVTGGGSPAAIWKDFMSAALPRLNAPSIPNGPPMPEGWV
                     APDPVGDLMGQVEDPYGGEVVAPVDPEAGEPYNGAGQQLAPRIEVRPTQQPRREDGAQ
                     RLETLPPEKRSDPLFF"
     gene            complement(5612..6268)
                     /locus_tag="VF633_06425"
     CDS             complement(5612..6268)
                     /locus_tag="VF633_06425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8470729.1"
                     /translation="MSHAPAAHPSANRIVDLYSEKADGWIADRKRTLGSAGSGLTEGG
                     WLERFMAALPPGGSVLDVGCGSGWPVAATLLERGFRVVGVDPAPDLLVHATETLPDGE
                     WLAGDMRTFDLGERRFDGLLAWHSLFHLAPHDQRIALPRILAHAADVCVFMTSSGPAE
                     GETIGEWRGEPLYHASLDPAEYRAILEANGFLPDAVDPSEQDGHGIIWIARRSGLTNP
                     "
     gene            complement(6281..7231)
                     /locus_tag="VF633_06430"
     CDS             complement(6281..7231)
                     /locus_tag="VF633_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8470730.1"
                     /translation="MNSQLITGLVAALGLLANIASVGSASAQDGHAAHSEQAAGHRHA
                     DFTSDRLHVRVDGEEGGRDIILIPGLSSSPEVWQGTVDHLGGGWRVHRIHIQGFAGAP
                     VGGNGTGPVAAPVAEEIARYITENKLDQPVVVGHSMGGTIGMMLAARHPDLIGKLMVV
                     DMVPFMGAMFGPPGTTAESVIPTADAIGGGMASATPEQYQQRAAATVTGMISTESKRA
                     GPLHDTTTSDQQVASHAFRELIATDLRPELTKITVPTKVVYVKFNDPRMTADIVDGIY
                     QAAFANLPGVKLQRIDDSAHFIMLDQPDAFHAALDAFVAP"
     gene            7480..8601
                     /locus_tag="VF633_06435"
     CDS             7480..8601
                     /locus_tag="VF633_06435"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX8470731.1"
                     /translation="MPSADSASISSNAPEAKAPPTAFGKGFVLDAWYFVALSRDVAKA
                     SLKRHEIMGEPVLIGRTNAGAVYGMRDICPHRAAPLSAGRLVDKPGEGETIECPYHGW
                     RFRTDGVCAAIPSLVEDQAFEAGRVRVRSYPVVESQGMVFVWMRSDPRSDAAPDHEPP
                     VFPGVVGGDAKLVEWMDFDSHIDHAVVGLMDPAHGPYVHQQWWWRSEHSMHEKAKMFE
                     PREHGFAMVRHAPSSNSRAYKILGGAPATEITFKLPGYRWEHIQVGEKQVLALTCLTP
                     VTETKTRITQIFWSDHWVFSLAKPFLRMGVVAFLKQDGGMVNLQNEGLRYDPALIWID
                     DADKQAKWYQQLKREWTKSRVEGRGFVNPVKPGMLRWKS"
     gene            complement(8645..9313)
                     /locus_tag="VF633_06440"
     CDS             complement(8645..9313)
                     /locus_tag="VF633_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8470732.1"
                     /translation="MSQNAAIAEAPASSNDVSANDGPRMNRRQAAKVRTRQKVLDAAR
                     GLFAERGYEPATIRDIAKGAGMSTGAVFANFQDKAELFEAVLTEDMTKLADTVKAGAG
                     EGSVKDRLSAALTAGYHGSLDQLPLFQAIVARSWFQPLAAETRARAVTKQLLAVITQI
                     LRDGVGKGELSQDADVPLLTELLWDAYLSNYRRAAYDSWSEQQLSNHVAKQIDVIIAG
                     ALTR"
     gene            complement(9586..11415)
                     /locus_tag="VF633_06445"
     CDS             complement(9586..11415)
                     /locus_tag="VF633_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8470733.1"
                     /translation="MAVGHGGGGEYKDLVVFLAAAGVVVPLFSRLRISPVLGFLAAGV
                     VLGPHGLGQLTGTVPWLSWFTISDPAQIAQLSELGVAFLLFMIGLELSWERLKSMRRL
                     VFGFGLLQVLICALVLAGGFILLGQTLAGAAVLGMGLALSSTAVVMPVMAETGRLKGA
                     TGRSTFAVLLAQDLAVAPILITVSFLAAAAHPGGLLEPSQIGRTLFTLAPAAIGLALI
                     VLLGRVVLRPLFRSVARSRKRTHGGQELFVALCLLVVVGTGVAAQAAGLSMSIGALIA
                     GLLIAETEYRREVEINIEPFKGLLLGVFFVGVGIGLDLNVLAANPLFVFGLAIAITAI
                     KAAIIFGLARLWKLSARTAIETALVLGPAGEFAFVVLTTGMVEGIAAPAFTQSVLVSA
                     TLSMFCVPLMAWLGQTLARRGERAPGLPLETLTPVIKDGAVLIVGFGRVGRLVGEMLA
                     EHKVPFIALDTDPAAVTRARREGFDIYYGDAGRAEMLTLCGIDTARALVVTMDAPTKV
                     DEVVIAARALRDDMTLIARARDDRHAARLYGLGATDAVPETTEASLQLAENVLVDVGI
                     PMGLVLASIHEKRDVFRAVFQAAIPADQRGRKTRALRGSLERK"
     gene            11645..>12545
                     /locus_tag="VF633_06450"
     CDS             11645..>12545
                     /locus_tag="VF633_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8470734.1"
                     /translation="MKTLLLGAALGALLLPAAAFAQDFSAQNDTHECIDDGCTMVSLF
                     QSGPTGSGYQGTTAPKYGTWGFDLAGRDTTVNPGDNFMEYANGTALKSLVIPADRTSY
                     GSFALLRELSDNRMKELVSGLAARTDLTPGSDEAKIADAYRSYIDEARIEALDAQPLQ
                     PYLTAIRAADTRDKMAVYMGGTQGRLGASIFRTGITIDAKAPNRYVVAAGQGGIGLPN
                     RDYYLSPLYADKKVKYQAYVERMLTMIGWDKPAENAAAIVALETSIAQTHWTPIENRN
                     RDKTYNEYTIAKLTEEAPGFPWQA"
BASE COUNT         2232 a         3963 c         4194 g         2156 t
ORIGIN      
        1 ctccgtgctc agatcgatga gcaattgcca gtaaccaccc atccatcggt aacaggaccg
       61 ctcccacacc gaggtgtcga gaaaagccaa acgatctccg taggcgagga tattctccgc
      121 gatggctttc gcgatctcgg catcaatgcg cccgacgcct tcgatctcat gctcaaggag
      181 cctgaaatca ccgtccatga aggcgccgat gaccttgtga aaaacggggt gtaacgactc
      241 cggaacggag tgttcctgat cgtcattgtc cagcgtgatc gcgctcagcc ccatcgaatt
      301 ttctttctga ctggttttag cttgtcggaa tcggattggt gcgcgggtct gggccgttgg
      361 tgacgacgcg gtggcggtcg ccgaggtcct tgacgacgat gacgtccttg aagcccgccg
      421 cctcgaacag ggccttgacc tgcgggccct ggtcccagcc aatttcgacg gcgaatacgc
      481 cgccgggctt gaggatgcgc atgatttccg gggccaactc gcgataggct tcaagaccgt
      541 cggggccgcc gtccagcgcc agcaaaggat cgtgctcgcg cacctcaggg tcgaggccgg
      601 ggatgtcccc ggaagggata tagggagggt tcgagaccac cagatcgaag ctgtcgtcgc
      661 cgaaacccgc cgcccattcg gtgcgcagga aggtcgcgcg gccatcgagg tcgaggttgg
      721 cggcgttctc gcgagcgacg gcgagagcct cggacgagat gtcggtgccg acgcccttcg
      781 ccgcagggcg ctcgctgagg atcgccagca ggatggcgcc ggagccggtg cccagatcga
      841 tgacgttgaa ggtttcgttg cgggcgtggg ccagcatgac gatgtcgaga atggtctcgg
      901 tgtcggggcg cgggctcaga acgtcaggcg tgacgttcag catgatcttc cagaagccct
      961 tccggccgag gatgcgcgag accggttcgc gcttcagccg acgctctaca tagccgtcca
     1021 gaaccgcctg ctggtcatcg gtcaggaggc gatagggatc ggtcagaatg tccatgcggc
     1081 tggcgcccga ggcggcctcg agcagcagtc gggcgtcgat cgacgggctg tcgatgcgcg
     1141 cagacttcaa ctgggcttgg gcggatttcc atgccgagac gagcgtggga ccggacgagg
     1201 gcggcgttgc tggggcgggg gaagaggtcg tgtcgttcat gcgggtctcg ctaccggatc
     1261 gccgaccacg atgcgacccc ccgtttcgat cgaaacgtag aggccacaaa acacatggcc
     1321 gtagttgtcg aacaacgcgc gaacgacctc gaggtcgcgc acgccggtcg ccggatcgac
     1381 gtcgatggcg gcgcaacgga cggtcggctt ggcgccgcgc aggaccgtct cgccgatgga
     1441 tatcgactgg tcggaccagt cgttctcgac ccacggctcc agcccctcga tccagatatt
     1501 ggcgcggaat cgcaaggggt cgaggggctg gcctaaacgg cgttcgagat ctcgcacgct
     1561 gttcagattc aggatcgaga cgcggccttt ggacgagtcc atgaagcgat ggctcggccc
     1621 ggcggcgatc aagcgcagcg ggccgagggc ttcttcgccg agaaaatcgg ccagaactgt
     1681 cgcgaacgca gccccatcat ccggctcgtc gaggcggacg cagagccttt gatcgtcgat
     1741 ctcggcagtg aggatgttgg tcgcctcgtc ccaacaggtg cggacgcggg ccagcatcgc
     1801 ctgccgcatc aggacggtga aggcttgctt gggaatttgc tcgggggagc cgggatcgta
     1861 gccggacggt ccattttcca tcgcccacat tcgatcgccg ggaaaccagc cgtcggtggt
     1921 caggatcgcc tgcgtgaggg gctcgggcgt gaagcccttg accggatggc gatagagggc
     1981 ggcgaccaca gtcattgaag cctgactgcc tgacgaacgg ccggagagca agttgaggcg
     2041 gaggttcggc agggggcgct ggaacgcggc ggcagcgtgg ccgttcaggc gacgatggct
     2101 ttctctccca agaccccctt caagcggccc gtcggaagcc gcggctcgat ctggcgcttt
     2161 gtcggggcgg ctgtgggcgg attggcgacc ggagccggcg ccgcgcatct gctttatacc
     2221 caggggcaca aggtcggaat cgaactcagg cccaggcgac ctgagccgct cacaccgaag
     2281 gcgcccacac ccgaggatta tgacgcccgc gatcccgggc gaggacggct ggcgcaacgg
     2341 cctgagcata ttccgcacaa gggctggatc gacatcctgt ggcgcgtcgg cgcggcctat
     2401 ttcggcgacc gggtcgggtt cgtcgccggc ggggtgacct tctttctggt gctgtcgctg
     2461 ttcccgacgc tggcggcctt cgtgaccctg tacggactgt tcgccgaccc cgccgacgcc
     2521 tggagccggc tgcagttcct ctattccata ctgccgtcga acgtggctga gttccttggg
     2581 gagcaaatgc agcggctggc cctgaacccg tcgggccagc tgaccttcac gctggtctgg
     2641 accctgctgt tgtcgctgtg ggcagccaac ggcggggtca agacgctctt ctacggcctg
     2701 aacgtcgcct atcacgaggt cgagaagcgc aatctggttc gctacaactt gatctgcatg
     2761 gccttcaccc tttcggggct aacggcggtg ctggtaacgg ccggtctcgt ggtcggagcg
     2821 ccgatcgtgc tggcggtatt tgggctgaac gaggagttcg gcgccctcgc ctggctgcgg
     2881 tggccgctcc tgttggtcgg ctacatcggc gccctgaccc tgatctatcg tttcggcccc
     2941 tgtcggcaga aggcgcgctg gcgctggctg acgccggggg ccgtcttcgc cgcgaccctg
     3001 agcgtgacgg tttcgttcct gttcagctgg tacctgtcgc acttcgtgcg gctggactcc
     3061 tacggaccgt tggcggcctt catgggcttc ctgctgtgga tctggatttc ggttcaaatc
     3121 atcctgatgg gcgccgagat gaacgccgag atcgagcatc agaccgcgct cgacaccacg
     3181 acaggcgccg cgcgtcctat cggcgaacgc ggcgcgttgg tcgccgatac ggtggggccg
     3241 agacgcggat cgccggctgc cttggccttc acgctgaaac atgcggaggc tgtcgccgac
     3301 cgggtggtca agaggcgggc gaagaccgcc gtggtgaagc ctgtcgaaga accctgaaag
     3361 acaggcctag aagaacaggg ggtccgagcg tttttccggg ggcagggtct cgagccgctg
     3421 agctccatct tcgcgacggg gttgctgggt cgggcgaacc tcgatgcgag gggccagctg
     3481 ctgacctgcg ccgttgtagg gctcgcccgc ttcaggatcg accggcgcca cgacttcacc
     3541 gccgtagggg tcctcgacct gacccatcag atcgcccacc ggatcaggcg cgacccagcc
     3601 ctcgggcatg ggcgggccgt tggggatgga cggtgcgttc aggcgcggca gggcggccga
     3661 catgaagtcc ttccagatgg cggcaggcga accgccgccg gtgacgccgc gcatcggcgt
     3721 ggcgtcgtcc ttgcccaccc acacagccgc cacgaagccg ccggtgtagc cgacgaacca
     3781 agcgtccttg tagtccgagg tggtgcccgt cttgccggcc agatcgcgac cggcgatggc
     3841 cgccgaggtc ccggttccgc cggtcatcac gccgcgcagc atctggttca tatagtagag
     3901 cggcgggttg ttgatcgtct gctgcccgat cgcgccggag tttccgtgct ggtagatgac
     3961 ccgtccctgc ggcgtgcgga tgcggctgat gccgtacgct tcgacgcgac gaccgccgtt
     4021 ggcgaaggcg tcataggcgg tggccatctc gatcggagag acgtcggacg tgcccaacgc
     4081 catggcgggg tccaggctga tgcggctggt gatgcccaga cggcgcgcga cgcgtgagac
     4141 actgtcgcgc cccaccttgt cggcgaccga cgcggcgatg gtgttggtcg aatgggccac
     4201 ggcatgcgcg atggtggtgg gaccgccata ggtgccgcca tagttcttgg gcgaccatgt
     4261 gccgatggtg acaggctcgt cgacgaccgg cgtttgcggc gtgtagccgg cctccatggc
     4321 agccagatag acgaagggct tccacgacga accggcctga cgctgggcgt cgacggcgcg
     4381 attgaactgg ctgtcagcgt atgaggcgcc gccgatcatg gcacggaccc ggccgtcgcc
     4441 atccagcgcc accagcgccg cctgctgcac gcccttggag gcgtcgcggg tgaggatgcg
     4501 gtttacggcg cgctcggccg aggtctggat ggtcaggtcg agggtggttt cgacgaccat
     4561 gtcttcggtg ggttcaccga ccagcccgcg gatctgcttg tcgagccaat cgatgaaata
     4621 ctgggcgtgc tggctggcca gggtgcgtga gacccgcacg ggctcctgca cagcctgatc
     4681 tcgctgggcg gaggtgatga cgccggtctc gaccatctgg tccagaacca cggtggcgcg
     4741 ggttgcggcg cgtttcgatt cggacaccgg cgaatagcgc gacggcgcct tcagcagccc
     4801 ggccagcagc gcggcctcgc ccacggtcag atccttggcc gatttgtcga aatagcgttg
     4861 cgacgccgcc tcgatgccat aggcgcctgc gccgaaatag acccggttca gataaagggc
     4921 gagaatctct ttcttggtga atttcagctc cagccagacg gccagcatca actcctgaac
     4981 cttacgcttc atgttctgat cgggcgtcag gaacaggttc ttggccaact gctgggtcag
     5041 ggtcgagccg ccctgaacga cgcgacctgc gcgcatgttg cgaaccatgg cgcggctcat
     5101 gccgatgggg tcgaagccgg ggtggtacca gaaccggcgg tcctcgatgg cgatgaaggc
     5161 ggccggcaca tagtccggca ggctgtcgag atcggcgggc ggcgcctgtt gcgtgccccg
     5221 cgtggcgatc agggcgccgt tgcgatcaag ataggtgatc gagggctggc gatcgacgtt
     5281 gtagaggctg gaggtgtcgg gcaggccgcg cgcgaacacg gcgaagaaga cgaccaggaa
     5341 gatcacgccc cagaccgcca ggaccgcacc ccagtagaag aatttctgca gggcgctccg
     5401 ctgcggtttt gcgccggcgc cgccttgcga accctgtcta ccgccgtaac cctgaccggc
     5461 catcgaaatc ctcaagtccg ctgtagaaca gccttagccg gtcccgcgtc tatctcaaac
     5521 gctcgccatc aaaacagctc gcctggtgaa tgggccatga agacgtagcg ggtacctgag
     5581 ggcgcttttc gcctaaagga tggccggcgg gttacggatt ggtcagcccg ctcctgcgcg
     5641 cgatccagat tatgccgtgt ccgtcctgct cggaaggatc gactgcgtca ggcaggaagc
     5701 cgttggcttc gaggatggcg cggtattcgg ccgggtccag actggcgtga tagagaggct
     5761 caccacgcca ttcgccgatt gtctcgccct ccgctgggcc actggaagtc atgaagacac
     5821 aaacgtcggc tgcgtgagcc agaatgcgcg gcagcgcgat ccgctgatcg tgaggagcca
     5881 ggtggaacag gctgtgccag gccagcaaac catcgaagcg acgctcaccg agatcgaacg
     5941 tccgcatgtc gccggctaac cactccccgt caggcagagt ctccgttgca tggaccagca
     6001 ggtcgggcgc cggatcgacc ccgacgaccc ggaagcctct ctcaagcagc gtcgccgcca
     6061 ccggccagcc ggagccgcag ccgacatcga gaacgctgcc gcccggtggc agagcagcca
     6121 tgaaccgctc gagccagccc ccctccgtca ggcccgaccc ggcggacccg agcgttcgct
     6181 tgcggtcggc gatccacccg tcggccttct cagaatagag gtcgacgatc ctgtttgcag
     6241 aaggatgggc ggccggagcg tggctcatcg cccgagcggc ctacggcgcg acgaaggcgt
     6301 cgagcgccgc atggaaggca tcgggctggt caagcatgat gaagtgggcg ctgtcgtcga
     6361 tgcgttgcag cttgacgccg ggcaggttgg cgaacgcggc ctgatagatg ccatcgacaa
     6421 tgtcagcggt catgcgtgga tcgttgaatt tcacatagac caccttggtc gggacggtga
     6481 tctttgtcag ttccgggcgc aggtcagtgg cgatcagctc acggaaggca tgggatgcga
     6541 cctgctggtc acttgtggtg gtgtcgtgca gggggccggc gcgtttggat tctgtactga
     6601 tcatcccggt gacagtcgcg gcggccctct gttgatattg ctccggcgtg gctgaagcca
     6661 tgcctccgcc gatcgcgtcg gcggtcggga tcacgctctc ggcggtggtt ccgggagggc
     6721 cgaacatcgc gcccatgaac ggcaccatat ccacgaccat cagcttgccg atcagatccg
     6781 gatggcgagc agccagcatc atgccgatag tcccgcccat ggaatgacca accacaactg
     6841 gctgatcgag tttgttctcg gtgatgtagc gggcgatctc ctcggcgacc ggcgccgcaa
     6901 ccgggccggt tccgttgccg ccgaccggag cgccggcgaa gccctgaatg tggatgcggt
     6961 ggactcgcca gccgccgccg agatgatcaa ccgtaccttg ccagacctca ggtgaggagc
     7021 tcaggccggg gatcaggatg atgtcgcgac cgccttcctc gccgtccacg cggacatgca
     7081 agcgatcgga tgtgaagtcg gcgtggcggt ggccggcagc ctgttcgctg tgggccgcat
     7141 gaccgtcctg tgcgctggct gagccgacgc tcgcgatgtt ggcgaggagg ccgagagcgg
     7201 cgacaaggcc ggtgatcaac tgggagttca ttcgagttcc tcttatatag tgtacgtcac
     7261 acagtatgtg tcatatactg tgtgacggtg tcaaggtttc gctgaaccct gttcgtacat
     7321 agggcgatcc tcccggtctg catgatcaaa tgggcgcgat cgcataggcg gaacacggca
     7381 atgaagtttg cttccttgct actgctgggg aggagagcct catccttcac gctccgcctg
     7441 ccaccttctc ccgcagaggg agaaggacta tgattagcca tgccttcagc cgacagcgcc
     7501 tccatctcct ccaacgcccc cgaagcgaag gccccgccga cggcgttcgg caaggggttc
     7561 gtgctcgacg cctggtattt cgtggccctg tcgcgcgacg tcgccaaggc ctcgctgaaa
     7621 cggcacgaga tcatgggcga gccggtgttg attggccgca ccaacgctgg agccgtctac
     7681 ggcatgcgcg acatctgtcc gcaccgcgcg gcgccgttgt cggccgggcg gctggtcgac
     7741 aagccgggcg agggcgagac tatcgaatgc ccctatcacg gctggcgttt ccgcaccgac
     7801 ggggtctgcg ccgccattcc ctcgctggtc gaggatcagg cgttcgaggc gggccgcgtc
     7861 cgggtgcggt cctatccagt ggtcgagagc cagggaatgg tgttcgtctg gatgcgctcg
     7921 gacccgcgct ccgacgccgc gccggaccat gagccgccgg tgttccccgg cgtggtcggc
     7981 ggagacgcca agctcgtcga gtggatggat ttcgacagcc acatcgacca cgcggtggtc
     8041 gggctgatgg accctgctca cggtccctac gtgcatcagc agtggtggtg gcggtccgag
     8101 cattcgatgc acgagaaggc caagatgttc gagccgcgcg agcatggctt tgcgatggtg
     8161 cgacacgctc cgtcatcgaa cagccgggcc tacaagatat tgggcggcgc gcccgcgacc
     8221 gagatcacct tcaaactgcc cggctatcgg tgggagcaca tccaggtggg tgagaagcag
     8281 gtgctggccc tgacctgcct gacgccggtg accgagacca aaacccgcat cacccagata
     8341 ttctggtccg accattgggt gttcagtctg gccaagccct tcctgcggat gggcgtggtc
     8401 gccttcctca agcaggatgg ggggatggtg aacctgcaga acgaggggct gcgctacgac
     8461 cccgccctga tctggatcga cgacgccgat aaacaggcga aatggtacca gcagctgaag
     8521 cgagaatgga caaaaagccg cgtcgaggga cgcggcttcg tcaacccggt caagcccggg
     8581 atgctgaggt ggaagagcta gatctcctcc ccgtcgctgc gcgacaggga ggagacttct
     8641 tgccttagcg cgtcagggcg ccggcgatga tgacgtcgat ctgcttggcg acgtggttcg
     8701 atagttgttg ctccgaccag ctgtcgtagg cggcgcggcg gtagttggac agataggcgt
     8761 cccacaacag ttcggtcagc agcggcacat cagcgtcttg cgacagctcg cccttgccga
     8821 cgccgtcacg caagatctgg gtgatgacgg caagcaattg cttggtcacg gcgcgggcgc
     8881 gggtctcggc tgccaagggc tggaaccacg agcgggcgac gatggcctgg aacagcggca
     8941 gctggtccag tgagccatgg taacctgcgg tcagagcggc ggagaggcga tccttgaccg
     9001 aaccctcgcc ggcgccggcc ttcaccgtat cggccagctt ggtcatgtcc tcggtcagga
     9061 ccgcttcgaa cagttcggcc ttgtcctgga aattggcgaa cactgcgcca gtcgacatcc
     9121 cggcgccctt ggcgatgtcg cggatggtgg ctggctcata gccacgctcg gcaaagagac
     9181 cacgggcggc gtccagcact ttttgacgag tgcggacctt ggcggcctgg cgacggttca
     9241 tgcgcgggcc gtcgtttgcg gagacgtcgt tggaggaagc cggggcttcg gcgatcgcgg
     9301 cgttctggga catggagaaa tgctttactc aaacccggcg acgggggacc gccggggacg
     9361 atgggggaat ggggcaacgc gcacgccgca gcttcgccat atggcgacac tgcgggtttt
     9421 cgaaggacgc tacccgagaa ccgcggtctt catcatggaa cgatgacgaa aatggggtcc
     9481 aaacattaac gaggcggttt caacgacgtc atgcctcaga cgtaagcgct ggctcgacca
     9541 aggggcctgg cattccgcaa ggcaacctcg cccagtcatc gccgattatt ttcgctcgag
     9601 cgatccgcgc aaggcccggg ttttacggcc ccgctgatcg gcggggatcg cggcctgaaa
     9661 cactgcacga aacacgtccc gcttctcatg gatagacgca agcaccagcc ccatggggat
     9721 accgacatcc accagaacgt tttcggcaag ttgtaagctg gcctctgtcg tctcgggcac
     9781 ggcgtcagtc gccccgaggc cgtacagtcg cgcggcatgt cgatcatcac gggcgcgtgc
     9841 gatcagcgtc atgtcgtcgc gcagggcccg ggcggcgatc acgacctcgt ccaccttcgt
     9901 cggcgcgtcc atggtcacga ccagcgcgcg ggctgtgtcg atgccgcaca gggtcagcat
     9961 ctcggcccgg cctgcgtcgc cgtaatagat atcgaagcct tcgcggcgcg cccgggtgac
    10021 cgctgcgggg tcggtgtcca aggcgatgaa ggggaccttg tgctccgcca gcatctcgcc
    10081 gaccaggcga ccgacgcgac cgaagccgac gatcaggaca gcgccgtcct tgatcaccgg
    10141 ggtcagggtc tcgagcggca ggccgggggc cctctcgcca cgcctcgcca gcgtctggcc
    10201 tagccaggcc atcagaggga cgcagaacat actgagcgtc gccgagacca gaacgctctg
    10261 ggtgaaggcc ggggcggcga tgccttcgac catgccggtg gtcaggacca cgaaggcgaa
    10321 ctcacccgca ggcccgagca ccagcgccgt ctctatcgcg gtgcgggcgc tgagcttcca
    10381 cagccgggcc aggccgaaga tgatcgccgc cttgatggcg gtgatggcga tggccagtcc
    10441 aaagacgaac agcgggttgg cggccagcac gttgaggtcg aggccgatgc cgaccccgac
    10501 gaagaatacg ccgagtagca gtcccttgaa gggttcgatg ttgatctcga cctcacgccg
    10561 gtactcggtc tcggcgatca gcagtccggc tatcaatgcg ccgatgctca tcgacaggcc
    10621 cgcggcctga gccgcgacgc cggtccccac caccaccagc agacacaggg cgacgaacag
    10681 ctcctgaccg ccgtgcgtac gcttgcggct gcgggcgacg gatcggaaca gaggacggag
    10741 cacgacccgg ccgagcaaga cgatcagggc gaggccgatc gcggcgggcg cgagggtgaa
    10801 gagggtgcgc ccgatctggc tgggctcaag caggccgccg ggatgcgcag ccgccgcgag
    10861 gaacgagacg gtgatcagga tcggggcgac tgcaagatcc tgagccagaa ggacggcgaa
    10921 ggtcgaacga cccgtcgccc ccttgagccg gcccgtctcc gccatgactg gcatgaccac
    10981 ggccgtggag gacagggcga gccccatgcc gagcaccgcg gcgccggcga gcgtctggcc
    11041 aagcaggatg aagcctccgg ccagcacgag cgcacagatc agcacctgca gcagcccaaa
    11101 gccgaagacg agacggcgca tcgatttgag ccgttcccac gagagctcca gcccgatcat
    11161 gaacagaagg aaggcgacgc cgagttcgct gagctgggcg atctgcgccg ggtcgctgat
    11221 cgtgaaccac gacagccatg gcacggtccc ggtgagctgg cccaatccgt gcgggccgag
    11281 cacgaccccg gcggcgagga agcccagcac ggggctgatc ctcaggcggc tgaacagcgg
    11341 gactacgacc ccggcggccg ccagaaagac gaccagatcc ttgtattcgc cgcccccgcc
    11401 gtgtccgact gccattcgtc ccccgatccg atcaaatcgc cattgactga cctagaggac
    11461 aatcaagtct taacgcgaca gcagcgccat cccgtcgcgc tgaatcatga atctttttta
    11521 aggcgacacc cccctcgcct ggcgtaggtt cgccgccgat ccgccatttt ggcccgcgcc
    11581 gtacgtggct tccccagttg ctcattcgac gaaaatcgcc ggactttcag gacagtattt
    11641 gtacatgaag acgcttcttc tcggcgcggc ccttggcgcc ctgcttctac cagccgcagc
    11701 tttcgctcag gacttctccg cccagaacga cacccatgag tgcatcgacg acggctgcac
    11761 catggtgtcg ctgttccagt ccggtccgac cggctccggc tatcagggca cgaccgctcc
    11821 gaaatacgga acctggggct tcgacctcgc gggtcgcgac acgaccgtga accccggcga
    11881 caacttcatg gaatacgcca acgggaccgc gctgaagtcg ctggtcatcc cggccgaccg
    11941 cacctcctat ggctcgttcg ccctgctgcg cgaactgtcc gacaaccgca tgaaagagtt
    12001 ggtctcgggt ctcgctgcgc gaaccgacct gaccccgggt tcggacgaag ccaagatcgc
    12061 cgacgcctat cggtcgtaca tcgacgaagc ccggatcgag gcgctcgacg cccagccgct
    12121 gcagccttat ctgaccgcca ttcgcgccgc cgacacccgc gacaagatgg ccgtctacat
    12181 gggcggcaca cagggccgtc tgggtgcgtc gatcttccgc accggcatca cgatcgacgc
    12241 aaaagcgccc aatcgctacg tcgtcgccgc agggcagggc ggcatcggcc tgccgaaccg
    12301 cgactactat ctgtcgccgc tttacgccga caaaaaggtc aagtatcagg cctatgtcga
    12361 gcgcatgctg acgatgatcg gctgggacaa gccggccgag aacgccgccg ccatcgtggc
    12421 tctcgaaacc tcgatcgctc agacccactg gacgccgatc gaaaaccgca accgcgacaa
    12481 gacctataac gaatacacca tcgccaagct gaccgaagaa gcccccggct tcccatggca
    12541 ggcct
//
LOCUS       DASVTM010000236         8162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_503655,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000236 DASVTM010000000
VERSION     DASVTM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503655"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VF633_06455"
     CDS             complement(<1..500)
                     /locus_tag="VF633_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEX8470735.1"
                     /translation="MAMQPAFAEPRAQVRGEMDTALRTQLSRAVGDVDGAPANRFEAR
                     RRARSAVEGAQALLRSEGYYQATVEDVVEGEETPVAIVNVIPGPRFLIARPDIDWIAP
                     LPDPLVATAVSAETGLTVGAPGRAVDVIAAEGRIVAGLTRRGYADAATQPRRVVVDHA
                     AFTVQPT"
     gene            1165..1241
                     /locus_tag="VF633_06460"
     tRNA            1165..1241
                     /locus_tag="VF633_06460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1199..1201,aa:Lys,seq:ttt)
     gene            1428..1601
                     /locus_tag="VF633_06465"
     CDS             1428..1601
                     /locus_tag="VF633_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470736.1"
                     /translation="MQNILIGLGVLLLLGVIVAMALNPGRTRRAHFTDADSDGESDET
                     PEQKRRALRDRRP"
     gene            complement(1598..2113)
                     /locus_tag="VF633_06470"
     CDS             complement(1598..2113)
                     /locus_tag="VF633_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470737.1"
                     /translation="MNTSALTTARFLALISLGALATACSTTREADPTPMASGGAPQPI
                     RGYDWFFHPEATEAKLVYGVETSDDLRLGLTCEKGSGRVEITANAPHGVREIYLESGG
                     ETERFRAEGEASELADGDFLTAEATTAAPVFQRFRRVGWLAQWNGEKRETYVPHPATA
                     PTIDRFFAFCG"
     gene            complement(2131..2586)
                     /locus_tag="VF633_06475"
     CDS             complement(2131..2586)
                     /locus_tag="VF633_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470738.1"
                     /translation="MTYLQAAMASASLMLWPGPGHPGQVTGWTWTLYEGEGPLVLANE
                     MPDTPQLRTTFECTRGSGVARLSVYGGPLGGGFASVTSGPGAASAQVQAEPGRDGKLS
                     LAVRTDHPVFGQFLTTGDLAVTIGGQRQAVTVETAHLAKLRRFADLCGG"
     gene            complement(2624..3094)
                     /locus_tag="VF633_06480"
     CDS             complement(2624..3094)
                     /locus_tag="VF633_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470739.1"
                     /translation="MFKILVPAVAALGMVAFAAKTQAVASDEPASGAAASSVAAEPIM
                     GWHLSYEGTVAKLAYGVENSDQLVMMITCAPRHGSAVIFGDVLPASPRLVQTSAGSDM
                     DEARVPISDPALQSLARDGILPVVSATGPARIRATPNERRLVSGFLNYCDGARA"
     gene            complement(3254..6217)
                     /gene="uvrA"
                     /locus_tag="VF633_06485"
     CDS             complement(3254..6217)
                     /gene="uvrA"
                     /locus_tag="VF633_06485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX8470740.1"
                     /translation="MIEKHNFIRVRGAREHNLKGVDVDIPREQLVVMTGLSGSGKSSL
                     AFDTIYAEGQRRYVESLSAYARQFLELMGKPDVDLIEGLSPAISIEQKTTSKNPRSTV
                     GTVTEIHDYMRLLWARVGVPYSPATGLPIESQTVSQMVDKLTALPEGERLLLLAPVVR
                     GRKGEYKKEISEWQRSGFQRLKIDGQFFAIEDAPTLDKKFKHDIDIVVDRIVTKNGLE
                     PRYADSIQTALGLADGIAVAEWANADEGKEPRRITFSEKFACPVSGFTISEIEPRLFS
                     FNNPFGACPVCDGLGVKLAFDADLAVPDRDKTLHKGAVAPWSRGPSPLYTQTLQALAL
                     HYGFSMDVPWRELSAKAQNVILQGSGGDKIKFVYDDNARKYETVKTFEGVLPNLERRW
                     RETDSAWVREELGRFQSETPCDACHGKRLKPEALAVKIDGSDIADISTLSISKAHHWV
                     STLSERLTEKQLEIGRRILKEIGDRLRFLNNVGLDYLSLARASGTLSGGESQRIRLAS
                     QIGSGLTGVLYVLDEPSIGLHQRDNTRLLESLRGLRDLGNSVLVVEHDEEAIMTADYV
                     IDMGPAAGIHGGEICAEGTPAEVMANPNSLTGKYLTGEREIELPPEGRRPVNRKKMLK
                     ISGATGNNLKTVTGEIPVGLFTCVTGVSGGGKSTFTIETLYKAAARRLHNASDAPAPF
                     DRIEGLEHFDKVIDIDQSPIGRTPRSNPATYTGAFGPIRDWYAGLPESKARGYGPGRF
                     SFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCDVCKGKRYNRETLEIVFKGKSISDVL
                     DMTVEEAGHFFKAVPPIRDKMLTLTRVGLDYVKVGQSATTLSGGEAQRVKLSKELSKR
                     ATGKTLYILDEPTTGLHFEDTRKLLEVLQELVDAGNTIVVIEHNLDVIKVADYLLDFG
                     PEGGEGGGEIVAVGTPEQVAANDASWTGRYLKEVLDRHETRRKARVAALNAPALNAAQ
                     PGSKAKAAARIRKTA"
     gene            complement(6367..6804)
                     /locus_tag="VF633_06490"
     CDS             complement(6367..6804)
                     /locus_tag="VF633_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470741.1"
                     /translation="MRLMALPVCLVASLSLAACATVGADDVPSGPPTVIRAPGEPAPA
                     QAKLYADCLAASVVAGTYEKEPGADLLRLTCTGGPARAFYDGLAVWSTTGEGSEIVAE
                     GRTWRYTQKIEKSPFGLDDCSTDGTADYRCTIILTVGKFLSAQ"
     gene            7163..>8162
                     /locus_tag="VF633_06495"
     CDS             7163..>8162
                     /locus_tag="VF633_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8470742.1"
                     /translation="MGKSTLLRLLSGDQAPTGDTISRTGTFEILDQRHEPGPTECVAD
                     TLGVGTGAAVLERILAGEGTSEDLSDADWTLTQRIDDALAQVGLQGLALQRPSATLSG
                     GELTRIRLAGLLIIAPDQLLLDEPTNHLDANARRIVGEVLGRWTGGAVVVSHDRDLLR
                     RMDRIVELSSLGATIYGGNYDLYAERKAAEQAALERELAFAEQNVGRAIRENRKAGER
                     KVRRDRAGRAFAASGSAPKIVLGMMAERAEASGAREGHLAERRIEQADAALVEARERV
                     ERVRALFIPMPPTGLAAGRAVLAMKGATWATPEGRRIVGPVDLVVTGPRRIAITGAN"
BASE COUNT         1404 a         2658 c         2656 g         1444 t
ORIGIN      
        1 gtcggctgca cggtgaaggc ggcatgatcg acgacgaccc gacgcggctg ggtcgccgcg
       61 tcggcatagc ctctacgcgt caaacccgcg acgatccggc cctcggcggc gatgacatcc
      121 acagctcgcc ccggtgcgcc caccgtaagg cccgtctccg ccgaaaccgc agtcgccaca
      181 agtggatctg gcaaaggcgc gatccagtcg atatcgggcc gggcgatcag gaaacgcggg
      241 cccggtatga cattgacgat ggcgacgggt gtttcttcgc cctcgaccac atcctcgacc
      301 gtcgcctgat aatagccttc ggaccgcaac aaggcctggg cgccctcgac ggcggaacgg
      361 gcgcggcgtc tggcctcgaa cctgttcgca ggcgcgccgt caacgtcgcc gaccgcgcgc
      421 gacaactggg tccgcagggc ggtgtccatc tcgccgcgaa cttgggccct gggttcggcg
      481 aaggcgggtt gcatagccat tgccgatgca gcgaacccga acaggacaag gcgcacggct
      541 ccagcccgga gatggccctt tcgggagccg ccttgcatgg tctggccccg gtcggccggc
      601 tgaggtggcc gagaaatcaa gcttgaactt tcgtcggcgc caccgccccc cggggatccg
      661 taaccattcc ggcgtggcga ctgtatggcg cggatctgga ggcgggggat tcagtagaag
      721 agggtctcgc tttccggttt cacggtctct tctgatggcc gttcgtcggt cggcaacgcg
      781 ctggtgtcgg tcgcaggcgt ttgcggagtg ctggttgcgg cggacggagc ggcgctatcg
      841 ctcgctgcgg gcgcaacggg ggcaggttca ggcggggggg gagcggtttc cgtttgtggt
      901 tgacgaacgt cgtcgcagcc cgccagcacg gtcgcagcgc ctatagcaag cgcaagaatc
      961 agtttcttca tcgccgtcct ccccgatcgc cgcagaacgc gccttggact gacctgttcc
     1021 atagccgtcc tcgctccgcc ggtcacccct gagatgatgc cttgagacgg tccgacggca
     1081 tgaagacgat ttcctgcgct gttgatgcga tccaaggctt gacccgcatg gcctgatgca
     1141 acagaaggcc ccccacccca cggcgcgtcc ttagctcagt tggttagagc atctgacttt
     1201 taatcagagg gtcctcggtt cgagcccgag aggacgtacc atggggggct tcggcccccc
     1261 gctgacccgg atacaggatc tcctgacggc gaggctcgat cagctggggt tgggcaggct
     1321 ttcgagaccc acctgatacc cgccgtctcc caatctgttc cgccgcccga accggcaacc
     1381 gtcgccggtg cgtcgcgttg ggttccggtc acaacgggag cgccgccatg cagaacatcc
     1441 tgatcgggtt gggagtcctg ctcctgctcg gcgtcatcgt ggctatggcc ttgaatcctg
     1501 gccggacgcg tcgggcccac ttcactgatg ccgatagcga tggcgagtcg gatgagacgc
     1561 ccgaacagaa gcgccgggcg cttcgggatc gacgccctta gccgcagaac gcgaagaaac
     1621 ggtcgatagt cggcgcggtc gcgggatgtg gaacataggt ctcgcgcttt tctccgttcc
     1681 actgcgccag ccagccgacg cgccggaagc gctggaacac cggggccgca gttgtggctt
     1741 cggcggtcag gaagtcgccg tcggcaagct cggaggcctc gccttcggcg cggaatcgct
     1801 cggtctcgcc gccagattcc agataaatct cgcggacacc atgcggagcg ttggctgtga
     1861 tctccacccg gcccgaaccc ttctcgcagg tcaggccgag acgaagatcg tcgctggtct
     1921 cgacgccgta caccagcttc gcctccgtcg cttcggggtg gaagaaccag tcgtatcccc
     1981 tgatcggctg aggggcgccg ccggaagcca tcggcgtcgg atcagcctcg cgcgtcgtcg
     2041 aacaggccgt cgccagagcg ccaaggctga tgagggcgag aaaccgggca gtggtgaggg
     2101 cggaagtgtt cacgcagcga tccctgatgg ctaaccgccg caaaggtcgg cgaagcggcg
     2161 cagcttggca agatgcgctg tttcaaccgt cacggcttgt cgctggccgc cgatcgtgac
     2221 cgcgagatcg cccgtggtaa ggaactgccc aaagacggga tgatctgtgc gcactgcgag
     2281 gctgagcttg ccatcccgac ctggttcagc ctgcacctgc gcacttgccg ccccggggcc
     2341 cgatgtcacg cttgcgaagc caccgcccag cggtccgcca tagacggaca accgcgccac
     2401 cccggatccc cgggtgcatt cgaacgtggt tctgagctga ggcgtgtccg gcatttcgtt
     2461 ggccaggacg agcggccctt caccctcata gagcgtccat gtccagccgg ttacctggcc
     2521 tggatgtccc ggccccggcc agagcatcag agacgcgctc gccatcgcag cctgcaaata
     2581 ggtcatcaca cagcccccgc tgatttcgtc aaggctgcaa gggtcaggcc cttgcaccgt
     2641 cgcaatagtt cagaaatccg ctgaccagtc gacgctcatt cggcgtcgcc ctgatccggg
     2701 cggggccagt ggcgctcacc acgggcagga tgccgtcgcg ggcgaggctc tgcagcgccg
     2761 gatcactgat cggtacgcgc gcctcatcca tgtcgctgcc ggctgaggtc tgaacaagac
     2821 gaggggaggc gggcaagacg tccccaaaga tcactgccga cccgtgacgg ggcgcgcagg
     2881 tgatcatcat caccagttgg tccgagttct cgacaccata ggccagcttg gcaaccgtgc
     2941 cctcatagct gagatgccac cccatgatcg gttcggcagc cacgctggag gctgcggctc
     3001 cggaggcagg ctcgtcggac gcaacggcct gggtcttggc cgcaaaggcg accattccaa
     3061 gtgcggcgac ggcggggacc aggatcttga acatagtggc tctcgcgtta gcccaatcaa
     3121 cgcgacagct ccgcagcggt tcacatcagg ccgaccgcca ttaacgaatc actccgcgga
     3181 ggccgatttg gcgagtattc ggcgaactgc caatgaacgg cagcggcggt cgctgacgac
     3241 cgccgctcgt gccttaagca gtcttgcgaa tccgtgccgc cgcctttgcc tttgaccccg
     3301 gttgcgccgc gtttaaagct ggggcgttca gggcggcgac gcgcgccttg cgacgcgtct
     3361 catggcggtc gaggacctct ttaaggtacc gcccggtcca gctcgcgtcg ttggccgcga
     3421 cctgttcagg cgtgccgacc gcgacgatct cgccgccgcc ttcaccgccc tctggtccga
     3481 agtcgagcag gtagtcggcc accttgatca catcgaggtt gtgctcgatc accacgatgg
     3541 tgttacccgc atcgaccaac tcctgaagca cctcaagcag cttgcgggtg tcctcgaagt
     3601 ggaggccggt ggtcggttca tcgaggatgt agagcgtctt gccggtcgcc cgcttcgaca
     3661 gttccttgga cagcttgacc cgctgggcct cgccgccaga cagcgtcgtc gccgactggc
     3721 cgaccttgac gtagtccaga cccacgcgcg tcagggtcag catcttgtcg cggatcggcg
     3781 gcacggcctt gaagaaatgc ccggcctctt cgaccgtcat gtccagcacg tccgagatcg
     3841 acttgccctt gaacacgatc tccagcgtct cgcggttgta gcgcttgccc ttgcagacgt
     3901 cgcaggtgac gtacacgtcc ggcaggaagt gcatctcgat cttgatcagg ccatcgccct
     3961 gacaggcctc gcagcgcccg cccttgacgt tgaagctgaa tcgaccaggc ccgtagccgc
     4021 gcgctttgga ctcgggcagg cccgcgtacc agtcacggat cggtccgaag gcgccggtgt
     4081 aggtcgccgg gttcgaacgc ggcgtccggc cgatcggcga ctgatcgata tcgatgacct
     4141 tgtcgaaatg ctccagcccc tcgatccggt cgaacggggc aggggcgtcg gacgcattgt
     4201 gcagacgacg cgcggcggcc ttgtagagcg tctcaatggt gaaggtcgac ttgccgccgc
     4261 ctgacacccc ggtgacgcag gtgaacaggc cgacgggaat ctcgccggtg acggtcttca
     4321 ggttgttgcc ggttgcgcca ctgatcttca gcatcttctt gcggttgacc gggcggcggc
     4381 cctcaggcgg cagttcgatc tcccgctcgc cggtcaggta tttgcccgtc agcgagttcg
     4441 gattggccat gacctcggcg ggcgtgcctt cggcgcagat ctcgccgcca tggatgccgg
     4501 cggcggggcc catgtcgatg acgtagtcgg cggtcatgat cgcctcctcg tcatgctcga
     4561 cgactaggac cgagttgccg agatcgcgca gaccccgcag tgactcgagc aggcgggtgt
     4621 tgtcgcgctg gtgcaggccg atggacggct cgtcgagcac atacagcaca ccggtcaggc
     4681 ccgaaccgat ctgcgacgcc agccggatgc gctggctctc gccgccggac aaagtgcccg
     4741 aggcgcgcgc cagcgacaga tagtcgaggc cgacgttgtt caggaatcgc agccggtcgc
     4801 cgatctcctt gaggatgcga cggccgatct caagctgctt ctcggtcagc cgctccgaca
     4861 gggtcgacac ccagtggtga gccttggaga tcgacagcgt cgagatgtcg gcgatgtcgg
     4921 agccgtcgat cttgaccgcc agcgcctcgg gcttgagacg cttgccgtga caggcgtcgc
     4981 acggcgtttc cgattggaag cggccgagtt cttcacgcac ccaggcgctg tcggtctcgc
     5041 gccagcggcg ttcgaggttc ggcaggaccc cctcgaaggt cttcaccgtc tcgtatttgc
     5101 gggcgttgtc gtcatagaca aacttgatct tgtcgccgcc ggatccttgc aggatcacgt
     5161 tctgagcctt ggccgaaagt tcgcgccagg gcacgtccat cgagaagccg tagtgcagcg
     5221 ccagcgcctg cagcgtctgg gtatagagcg gcgagggacc acgcgaccac ggcgccaccg
     5281 cgcccttatg cagggtcttg tcgcgatccg gcacggccag atcggcgtcg aacgccagct
     5341 tgacgccgag gccgtcacac accggacagg cgccgaacgg gttgttgaac gagaacagac
     5401 gcggctcgat ctccgagatc gtaaagcccg aaaccggaca ggcgaacttc tcggagaagg
     5461 tgatccggcg cggctccttg ccttcatccg cattggccca ttcggcgact gcgatcccgt
     5521 cggccaggcc cagcgcagtc tggatcgagt cggcgtatcg cggttcgaga ccgttcttgg
     5581 tgacgatccg atccacgacg atgtcgatgt cgtgcttgaa cttcttgtcc agcgtcggcg
     5641 cgtcctcgat ggcgaagaac tggccgtcga tcttgagccg ctggaagccc gagcgctgcc
     5701 actcggagat ctccttcttg tactcgccct tacggccgcg aacgaccgga gccagcagca
     5761 acagccgctc gccctcgggc agggccgtca gcttgtcgac catctgcgag acggtctggc
     5821 tctcgatcgg cagtccggtc gcaggcgaat acggcacacc gactcgcgcc caaagcaggc
     5881 gcatatagtc gtggatctcg gtcaccgttc ccacggtcga gcgcgggttc ttcgacgtcg
     5941 tcttctgttc gatcgagatc gccggcgaca ggccctcgat cagatctacg tccggcttgc
     6001 ccatcagctc gaggaactgg cgtgcatagg cgctcaagga ttcgacatac cgccgctggc
     6061 cctcggcgta gatggtgtcg aacgccagcg aggatttgcc cgagcccgac aggccggtca
     6121 tcacgaccag ctgctcgcgc gggatatcga catccacgcc cttgagattg tgctcgcggg
     6181 cgccacgcac gcggatgaag ttgtgctttt cgatcatgtt gtccggagaa cgcgggagag
     6241 ccggacgagg tccgggacga cgacactata tgggggctga tccgcaggat tcagcaagaa
     6301 caatgtggga acattgcggc tgctgacaag gcggggggcg aaaccgtcgc ttcgacgccg
     6361 acgcgctcac tgagccgaga gaaacttccc gaccgtcaga atgatcgtgc agcggtaatc
     6421 ggcggttccg tcggtggagc agtcgtccag accgaacggg ctcttctcga tcttctgggt
     6481 gtaacgccag gtgcggccct cagcgacgat ctcggagcct tcgccggtcg tgctccagac
     6541 agcgaggccg tcatagaagg cgcgggcagg gccgcccgtg caggtcagac gcagcagatc
     6601 ggcgccgggt tctttctcat aggtaccggc aacgaccgag gccgccaggc agtcggcgta
     6661 gagtttggct tgcgccgggg caggttctcc gggagcgcgg atcaccgtag gcgggccgga
     6721 cggtacgtca tcggctccaa ctgtcgcgca ggccgccagg ctgaggctgg cgacgagaca
     6781 cactggcagg gccatcaaac gcatagagaa tcctccaaca gcgaagcctg tcacagagcc
     6841 gtgttcgccg ccagcagcct aatcctcttg cgtcctgcat agtgacgcgc cattcttcct
     6901 gcacggaggc caccattggc ctctggagtt tgtgcaggag gcctgaccat catgatcatc
     6961 gcgctggaaa tcggtaccgc cctttccctt atcgccatga tctcggtcta tgtctcgtct
     7021 ctccccaagc caaagcgcgt acccgtccgg gttcgtaacc acggaccgcg tcagcgctga
     7081 gacgcctgac ggtcaccgtc ttttcgacaa tctaaacctc gtcttcgggc gcgagcgtac
     7141 cggcctggta ggtcgcaacg gtgtgggcaa atccacgctg ctccgacttc tgtccggcga
     7201 ccaagccccg accggggaca ccatctcacg taccggcacc ttcgagatcc tggatcagcg
     7261 tcatgagccc ggcccgacag aatgcgtcgc cgacacgctc ggtgtcggaa caggcgccgc
     7321 tgtcctggaa cgcatcctcg ccggtgaagg aaccagcgaa gacttgtcgg atgccgactg
     7381 gaccctgacc caacggatcg acgacgccct ggcccaggtg gggctgcagg gtctggcgct
     7441 acagcggcca tcggcgacct tgagcggcgg tgagttgacc cgcatccggc tcgctggcct
     7501 cctgatcatt gcgcctgacc agttgcttct agacgaaccc accaaccatc tcgacgcaaa
     7561 cgcccgccgg atcgtcggcg aggttctggg ccgctggacc ggtggcgcgg tggtcgtcag
     7621 ccatgatcgc gacctgctcc gtcgcatgga ccggattgtc gagttgtcga gcctcggcgc
     7681 aaccatctac ggcggcaact acgacctcta cgcggagcgg aaggccgccg agcaagctgc
     7741 cttggaacgt gaactggcct tcgcagagca gaacgtgggc cgggcgatcc gggaaaaccg
     7801 gaaggccggg gagcgcaagg tacgacgtga ccgggccggc cgcgcgtttg cggctagtgg
     7861 atcggctcca aagatcgtcc tcggcatgat ggctgagcgc gccgaagcct ctggcgcacg
     7921 cgaggggcat ctggccgagc gccgtatcga acaggcggac gcagcgctgg tcgaggcgcg
     7981 ggaaagggtc gagcgggtgc gcgccctctt catacccatg ccgccgaccg gactggcggc
     8041 tggcagggct gtgctcgcga tgaagggcgc gacatgggcg acgcccgaag gccgccgtat
     8101 cgtcggcccc gtcgatctgg tcgtgaccgg gccccgtaga atcgcgatca ccggtgcgaa
     8161 tg
//
LOCUS       DASVTM010000237         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_979123,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000237 DASVTM010000000
VERSION     DASVTM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979123"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VF633_06500"
     CDS             <1..131
                     /locus_tag="VF633_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein MotB"
                     /protein_id="HEX8470743.1"
                     /translation="YSVAGKAGSDPLYPDDPSLAGNRRIAIVLLREAPVLPSDTSL"
     gene            251..988
                     /locus_tag="VF633_06505"
     CDS             251..988
                     /locus_tag="VF633_06505"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX8470744.1"
                     /translation="MTQHIPTRQLRFFMTAVAPCPYLPGMTERKVFANLPFSDGAHVN
                     DELTHAGFRRSQNIAYRPACEGCDACVSVRLPVPEFEFSRSQRKVLTRNADLSRALVE
                     AEATTEQFALLKRYLATRHPGGGMTAMTWADYVAMVEDTAVRTHLIEYRTVSPDGGPG
                     DLVAVTLADLLSDGLSMVYSYFDPDLERRSLGVFAILDHVRQAAIVNLPYVYLGYWVQ
                     GSPKMDYKANFRPMEVLRHHGWTRLED"
     gene            complement(1016..>1694)
                     /locus_tag="VF633_06510"
     CDS             complement(1016..>1694)
                     /locus_tag="VF633_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8470745.1"
                     /translation="GLQTPTLRIIETESMNAYASGLREGQYSVTVTRGLLNALDRDEL
                     EAVLAHELTHVINKDVRTMVIASIFAGIISVAAQLVLRGMLYVRGGGRRDNKNAMPLI
                     LIGLVIAAVGYGLAIVVRLMLSRTREYVADAGSVDMTKNPDAMISALRKISGRSTLKA
                     PDEVQGMFFDNQPDKEGIDSLFATHPSIEKRIAALVKYAGGRDLPPPSVTTAVPATQY
                     GAAVPFT"
BASE COUNT          275 a          625 c          507 g          287 t
ORIGIN      
        1 tctactcggt tgcgggcaag gcgggctcag acccccttta tccggacgac ccgtcgcttg
       61 cgggcaatcg ccgcattgcc atcgtcctgc tgcgcgaggc ccccgtcctg ccctcggaca
      121 ccagcctgtg acaggcgcgc ccctcctccg tggcgcgaac gcccttgcag cccggccacg
      181 aacggcgcca aatcgacgga tgaacgagcg tccgtcatac tgttggccag atcggatgcg
      241 gacctaggcc gtgacccagc atatcccgac ccgtcagctt cgcttcttca tgaccgccgt
      301 ggccccctgc ccctatttgc cggggatgac agagcggaag gtgttcgcca acctgccctt
      361 ctcggacggc gcccacgtca atgacgagct gacccacgcg ggcttccgcc gcagccagaa
      421 catcgcctat cgcccagcct gcgagggctg cgacgcctgc gtctcggtcc ggctgccggt
      481 gcccgagttc gagttcagtc gctcccagcg caaggtcctg acccgcaacg ccgacctgtc
      541 tcgcgcgctc gtcgaggccg aggccaccac cgaacagttc gccctgctca agcgctacct
      601 cgccacgcgc cacccgggcg gcggcatgac cgccatgacc tgggccgact acgtcgccat
      661 ggtcgaggac accgccgtcc gcacccactt gatcgagtac cgcacggtct cgcccgacgg
      721 cggccccggc gatctggtcg cagtgacgct ggccgacctg ctcagcgacg gcttgtcgat
      781 ggtctacagc tacttcgacc ccgacctcga gcgccgctca ctcggcgtct tcgccatcct
      841 cgaccacgtc cgtcaggccg ccatcgtaaa cctgccgtat gtctacctcg gctactgggt
      901 ccagggctcg cccaagatgg actacaaggc caacttccgc cccatggaag tcctccgcca
      961 ccacggctgg acgcggctgg aagactagaa gtacccctct cccgccggga gagggtcagg
     1021 tgaagggaac agccgccccg tactgcgtcg caggcacggc ggtggtcaca gatggcggcg
     1081 gcaggtctcg cccgcccgcg tacttgacca gcgccgcaat acgcttctcg atcgaggggt
     1141 gggtggcgaa caggctgtcg atgccttcct tgtcgggctg gttgtcgaag aacatgccct
     1201 gaacctcgtc cggcgccttg agcgtcgacc gccccgagat cttcctcaaa gccgagatca
     1261 tggcgtcggg gttcttcgtc atatcgaccg aaccggcgtc ggcgacatac tcgcgcgtcc
     1321 gcgacagcat cagccgcacg acgatcgcca ggccgtaacc gaccgccgcg atgaccagcc
     1381 cgatcaggat cagcggcatg gcgttcttgt tgtcgcgccg ccctccgccc cggacgtaca
     1441 acatgccccg cagcaccagt tgggcggcga cgctgatgat gccggcgaag atcgaggcaa
     1501 tcaccatcgt ccgcacgtcc ttgttgatga cgtgggtcag ctcgtgcgcc agcaccgcct
     1561 ccagctcgtc gcggtcgagc gcgttgagca ggcctcgcgt gaccgtgacg ctgtactgtc
     1621 cctcgcgcag gcctgacgca taagcgttca tcgactcggt ctcgataatc ctcagcgtcg
     1681 gggtctgcag gccg
//
LOCUS       DASVTM010000238         5138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_393300,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000238 DASVTM010000000
VERSION     DASVTM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393300"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF633_06515"
     CDS             <1..457
                     /locus_tag="VF633_06515"
                     /inference="COORDINATES: protein motif:HMM:NF023380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HEX8470746.1"
                     /translation="GHRIISHNGGIEGFNTWMARAEDGMTVVVLGNLNGPGPDKLGPA
                     LMTLAHGGEVALQSERQVIPVTAEVLNSYAGVYEASSTFAITVSVVDGRLMAQATGQP
                     AFELFPERTDGFFLKVVDAQLTFNRDASGAVTGLVLHQGGNDLPARKIS"
     gene            complement(519..1478)
                     /locus_tag="VF633_06520"
     CDS             complement(519..1478)
                     /locus_tag="VF633_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8470747.1"
                     /translation="MSNPTRIALILGATGGVGGETAAALARHGWTIRALTRDPEAARA
                     KRPEWTWVAGDALDRDAVVAAAEGVSIILHAVNPAGYRGWDRLVLPMIDNTIAAAKAS
                     GARILLPGTIYNYGPDAFPLLHEDLPQRPTTRKGAIRVEMERRLSAACADGVRTLILR
                     AGDFFGPRPGNNWFSQGLVTPGKPVRAIAYPGKTGVGHAWAYLPDVAETFAKLADIEA
                     ELEPFARYHFGGYWDADGTGMVEAIRRRAGGKAKVSPMSWPLMSMIAPFNETVRELME
                     MKPLWRRSVQLDNTRLTARLGAEPHTPLDEAVETSLKALGCLA"
     gene            1576..2481
                     /locus_tag="VF633_06525"
     CDS             1576..2481
                     /locus_tag="VF633_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8470748.1"
                     /translation="MNAPVPDTWLDWERQRAFLAVLGEGSLSGAARMLGLSQPTVRRR
                     IEDLERQVGAPLFTRSPSGLTPTGVARDLGQHARAMATAAEAFMRTASGDADAAGGVV
                     RIAASEVIGVEVLPPILAELRAAHPGLVLELSLGSRNEDLLKREADVAVRMSPPAQKA
                     LVARRIGAVRLGLFAHRRLIEAEGAPTTLEDLSRLPLIGYESETNSIRALRAMGLTLK
                     REDFVYRTDNDLAQLAAIRAGVGVGVCQTRLGDRNPDLVAVLPEAFSFDLDTWIVMHE
                     DLRRVRRVRLVYEALAKGLTTYIRG"
     misc_feature    2536..2589
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03058"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="sul1 is cis-regulatory element that is thought to
                     sense ions involved in sulfur or methionine metabolism;
                     They are found in Alphaproteobacteria; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03058"
     gene            2641..4125
                     /locus_tag="VF633_06530"
     CDS             2641..4125
                     /locus_tag="VF633_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX8470749.1"
                     /translation="MTLIDSLRREWLAQPGRDLLAGTVVALALIPEAIAFSIIAGVDP
                     AVGLYASFIIATTIAFVGGRPAMISASTGAMSLLMVVLVREHGIEYLFAASILTGLFQ
                     IVIGLLKLGRYVRFVSRSVMTGFVNSLAILVALAQLPQLIGAGWQTYALVAAGIAIIY
                     GFRRVTTAIPAPLVAVVVVSAFVMILGVDVRTVGDMGAMPRTLPIFHLPAVPLNWQTF
                     AIITPVAATLAFVGLLESLLTASLLDDITDTPSDKDRETRGQGIANIASALFGGMAGC
                     AVIGQSLMNVGAGGRGRLSTLWAGLFLLFLILVLQDWVARIPMAALVAVMITVAIGAF
                     DWGSITRIRSIPIQSTIVMIATTATVVITHDLSKGVVLGVVLSAIFFMRKVGKTVVVE
                     TVPTDQPDVLHYRVAGQLFFGSAGLFTAGFEHHGQPRWVVIDLTDAHLWDLTGVDALD
                     RVVFKYRRQGAEVEVIGMNDAAKTLVEQVGVHDKQGLPSGNLSG"
     gene            4122..>5138
                     /locus_tag="VF633_06535"
     CDS             4122..>5138
                     /locus_tag="VF633_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8470750.1"
                     /translation="MSGPSSIAVAGAGPTGLAVALMLARQGRRVVVHERFTEPHPVGA
                     GFMLQPTGLSVLDHLGLTPAIEALGQPLHHVFGREARRGRVVLDIHFADLKRPRPALG
                     VHRAAIFDVLYRACLAEGVSFETGREIVGASDGRLIDSAGRSGPVFDLIVDATGARSA
                     IAASLGARRRELEWGALWGTVPWPGAPFDEGALEQVYRGASKMIGVLPCGALPHGFGP
                     DSPDRLATFFWSLKHGDHATWRAEGLEAWKAEVLSLWPQTGPVLDAITDPDQLTLARY
                     GHHTLALPVANRLAVVGDAAHSTSPQLGQGVNMGLLDAMALAEAIRTHEALSDALTAY
                     ARARR"
BASE COUNT          756 a         1755 c         1729 g          898 t
ORIGIN      
        1 cggtcaccgg atcatcagcc acaacggcgg catcgagggc ttcaacacct ggatggcccg
       61 cgccgaggac ggcatgacgg tggtcgtgct gggcaacctg aacggtcccg gcccggacaa
      121 gctcggccct gccctgatga cgcttgccca cggcggcgag gtcgcccttc agtccgaacg
      181 gcaagtgata cccgtcacag cggaagtcct gaacagctac gccggagtct atgaggcgtc
      241 gtcgacgttc gccatcaccg tctcggtcgt cgacggacgc ctgatggccc aggccaccgg
      301 ccagcccgcc ttcgaactct tccccgaaag gaccgacggc ttcttcctca aggtcgtcga
      361 cgcccagctg accttcaacc gcgacgcctc gggtgcggtg accgggctgg tgctgcatca
      421 gggtggcaac gacctgccgg cgcggaaaat cagctaacgc ccgaacgaaa gatgatcgag
      481 gccccgctgg agacagcggg gccattgtcg ttttgggatc aggccaggca gcccagcgcc
      541 ttcagcgacg tttcaaccgc ttcgtccaaa ggcgtgtgcg gctcggcgcc gaggcgggcg
      601 gtcaggcggg tgttgtccag ttgcacggac cggcgccaca gcggcttcat ctccatcagc
      661 tcgcgcacgg tctcgttgaa cggcgcgatc atggacatga gcggccagga catgggcgag
      721 accttcgcct tgccgcccgc tctccggcgg atcgcctcga ccatcccggt cccgtccgcg
      781 tcccagtagc cgccgaaatg atagcgggcg aacggctcca gctccgcctc gatgtcggcc
      841 agcttggcga aggtctcggc gacgtcgggc agataggccc aggcgtgacc gacgccggtc
      901 ttgcccggat aggcgatggc gcgaaccggt ttgccgggcg tcaccagccc ttgcgagaac
      961 cagttgttgc cgggacgcgg cccgaagaaa tcgccggccc gcaggatcag ggtccgcaca
     1021 ccgtcggcgc aggcggcgga cagacgccgc tccatctcga cacggatcgc gcctttacgc
     1081 gtggtgggcc gctgcggcag gtcctcatgc aaaagaggaa aggcgtcggg gccatagttg
     1141 tagatggtcc ccggcagcag gatgcgcgcg ccggacgcct tggcggcggc tattgtgttg
     1201 tcgatcatcg gcagcaccag ccgatcccag ccgcgataac ctgccgggtt gacggcgtgc
     1261 aggatgatgc tgacgccctc cgccgctgca accacggcgt ccctgtcgag ggcgtcgcct
     1321 gcgacccagg tccattcggg ccgtttggcg cgagcggctt cggggtcgcg ggtcagggcg
     1381 cggatggtcc agccgtggcg ggcgagggcg gcggcggtct cgccccctac accgccggta
     1441 gcgccgagga tgagagcgat gcgagttggg ttcgacatga aagcctcctt tgggccggtg
     1501 ttcatgacga caaggtgagc cccatccgat cacaaagaaa ttgcccagga acgttgacct
     1561 gctagtctaa aacttatgaa cgcgcccgtt ccggacacat ggctcgattg ggagcggcag
     1621 cgggccttcc tcgccgtact cggcgagggc agtctgtccg gggcggccag aatgctgggt
     1681 ctctcccagc ccaccgtgcg ccggcgcatc gaggatctcg agcgtcaggt cggcgcgccc
     1741 ctgttcaccc gctcgccgtc gggcctgacc ccgaccggcg tcgcacgcga tctgggccag
     1801 cacgcgcggg ctatggccac cgccgccgag gccttcatgc gcacggcgtc gggggacgcc
     1861 gacgccgccg ggggcgtggt gcggatcgcc gccagtgagg tcatcggggt ggaggtcctg
     1921 ccgccgatcc tcgccgaact gcgcgcagcc catcccggtc tggtgctcga actcagcctc
     1981 ggtagtcgca acgaggacct gttgaagcgc gaagctgacg tggccgtacg catgtcccct
     2041 cccgctcaga aggccttggt cgcacgccgc atcggcgcgg tgcggctggg ccttttcgct
     2101 catcggcggc tgatcgaggc cgagggtgca ccgacaacac tggaggatct gagccgcctg
     2161 ccgctgatcg gctacgaaag cgagaccaac agcatccgcg ctctccgcgc catgggcctg
     2221 accctgaagc gcgaggattt cgtctatcgg acagacaacg acctggccca actcgccgcc
     2281 atccgggccg gggtcggggt cggggtctgc cagactcgtc ttggcgaccg taatcccgat
     2341 ctggtcgccg tcctgcctga ggcgttcagc ttcgatctcg acacctggat cgtgatgcac
     2401 gaggacctgc gtcgcgttcg tcgggtgcgg ctggtttatg aggcgctggc gaaaggcctg
     2461 accacctaca tccggggctg agcgctaccg gttcgcttgc cccgccaccg cttttgtcgc
     2521 atcaggcgga tgcacaaagg acccggccag accgggtggc tatcccggcc gccgatccgc
     2581 cgggaaacag cgcgggcctc agggcgtcgc tcgatccacg ccagcgactg aacccctcga
     2641 atgaccctca tcgactcatt gcggcgcgaa tggctggccc agcccggtcg cgatcttctg
     2701 gccggcacgg tcgtcgcgct cgccctgatc cccgaggcga tcgctttctc catcatcgcc
     2761 ggggtcgatc cggccgtcgg cctctacgcc agcttcatca tcgccacgac catcgccttc
     2821 gtcggcgggc gtccggcgat gatctcggcc tcgaccggcg ctatgtcgtt gctgatggtc
     2881 gtgctcgtgc gcgagcatgg catcgaatat cttttcgccg cgagcatcct gaccggcctg
     2941 ttccagatcg tcatcggcct gttgaagctg ggccggtacg tccgcttcgt ctcgcgctcg
     3001 gtgatgaccg gcttcgtcaa ctcattggcg atccttgtcg ctctcgccca gctgccgcaa
     3061 ctgatcgggg ccggctggca gacctatgcc ctcgtcgcgg cgggcatcgc catcatctac
     3121 ggtttccggc gcgtcacgac cgccattccc gcgccgctgg tcgccgtggt cgtggtcagc
     3181 gccttcgtca tgatcctcgg cgtcgatgtc cgcaccgtcg gcgacatggg cgccatgccc
     3241 cggaccttgc cgatcttcca tctgcctgcc gtgccgctga actggcagac cttcgccatc
     3301 atcacacccg tcgcggcgac cctggccttc gtcggcctgc ttgaaagcct gctgaccgcc
     3361 agcctgctgg acgacatcac cgacaccccc tcggacaagg atcgcgagac gcgcggtcag
     3421 ggtatcgcca acatcgcctc tgccctgttc ggcggcatgg cgggctgtgc cgtcatcggt
     3481 cagtcgttga tgaacgtcgg ggcgggtggg cgagggcggt tgtccacctt gtgggcgggc
     3541 ctgttcctgc tgttcctgat cctggtgctt caggactggg tcgcgcgcat tccgatggcg
     3601 gctctggtgg cggtgatgat caccgtggcc atcggcgcct tcgattgggg ctcgattacc
     3661 cgcatccgtt cgatcccgat ccagtccacc atcgtcatga tcgccaccac cgcgaccgtg
     3721 gtgatcaccc acgacctgtc caagggcgtc gtgctgggcg tcgtcctgtc ggcgatcttc
     3781 ttcatgcgca aggtcggcaa gaccgtggtc gtggagaccg taccaacgga ccagccggac
     3841 gtgctgcact atcgggtcgc aggccagttg ttcttcggtt ccgcaggcct gttcaccgcc
     3901 ggcttcgaac atcatggcca gccgcgctgg gtcgtcatcg acctgaccga cgcccacctg
     3961 tgggatctga ccggtgtgga tgcgctggac cgcgtggtgt tcaaatatcg caggcagggg
     4021 gctgaggttg aggtgatcgg catgaacgac gcggccaaga ccctggtcga acaggtcgga
     4081 gtgcatgaca aacagggcct gccgtccggg aacctcagcg ggtgagcggt ccgtcctcca
     4141 tcgcggtggc cggggccggt ccgacgggtc tggccgtcgc cctgatgctg gcgcggcagg
     4201 ggcggcgggt cgtcgtccat gagcggttta ccgaaccgca cccggtcggg gccggcttca
     4261 tgcttcagcc cacgggcctg tcggtgctcg accatctggg tctgacgccg gcgatcgagg
     4321 cgctgggcca gccgctgcac cacgtcttcg gacgtgaggc gcggcgcggt cgggtcgtgc
     4381 tggatattca cttcgccgat ctgaagcggc cgcgcccggc cctgggcgtc caccgcgccg
     4441 cgatcttcga cgtgctgtac cgcgcctgcc tcgccgaggg cgtgagcttc gaaaccgggc
     4501 gcgagatcgt cggcgcgtcg gacgggcggc tgatcgacag cgcgggtcgg tcaggcccgg
     4561 tcttcgatct gatcgtcgac gccactggcg cccgatcggc catcgccgcc agcctcggcg
     4621 cccgtcgtcg ggaactggag tggggcgccc tgtggggcac ggtcccatgg ccgggcgcac
     4681 cattcgacga aggcgcgctg gagcaggtgt atcgcggcgc ctcgaagatg atcggcgtcc
     4741 tgccgtgcgg cgccctgccc catggctttg ggccggatag cccggaccgc ctcgccacct
     4801 tcttctggag cctgaagcac ggcgaccatg cgacgtggcg cgccgagggt ctggaggcgt
     4861 ggaaggccga ggtcctgtcg ctgtggcccc agactggacc ggtgctggac gccatcaccg
     4921 acccggatca gttgacgctg gcccgttacg gccatcacac cctcgccctg cccgtggcga
     4981 accggctggc cgtcgtcggc gacgcggccc attcgaccag tccgcaactg ggtcaggggg
     5041 tcaatatggg cctgctggat gcgatggctc tggcggaggc gatccgaacc catgaggcgc
     5101 tgtcggacgc cctgaccgcc tatgcccgcg cgcgtcgc
//
LOCUS       DASVTM010000239        15221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_183565,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000239 DASVTM010000000
VERSION     DASVTM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 15221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15221
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183565"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VF633_06540"
     CDS             <1..832
                     /locus_tag="VF633_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8470751.1"
                     /translation="LLLPAALSTGWHRRAEPAVRFMVCWLVPAWVIFELAPTKLWHYT
                     LPTFGAIALLMAAALTRPIGMISRRVGAGLAVIAAMAICAITVYGLTAFGTSTAQTWA
                     AMTIVCAAVAGAGGAFILLNRAAVSALAVSVVFGVMAHAALAGTLRQLRPLSVAPMLV
                     EKMEQAGIAPDQGLIPGPVAITTFHEPSFVFLTGKDTELTDAAGAAKALAEGRPAIVE
                     ARDAEAFRQAMAVTGAGGRAVGNVRGHNYSSGDDVNLTLYAPTGAPAAPSAIAEGEAE
                     "
     gene            829..1746
                     /locus_tag="VF633_06545"
     CDS             829..1746
                     /locus_tag="VF633_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX8470752.1"
                     /translation="MKRPIQIVEVGPRDGLQNEKVILEPSIRAELVRRLEAAGARRIE
                     AVSFVHPKYVPQMAGAEEVMAELPKQPGRSRIGLVLNGKGYDRALGTAVDEVNVAMSA
                     TDGFGLKNQGLSVVQQVEMLAAIIAGRANAEGELGATPALSATISCVWGCPFDGEVST
                     DQVQDLVGRIAELGVAEIGLADTIGVGDPWSVTRKVEAARKAAPDATLRLHFHDTRNT
                     GLANAYAGIEAGIDVLDASVGGIGGCPFAPGSTGNIATEDLVYLLERGGYSTGYDLDA
                     LIVTATWIGEKIGRPAPSALSRAGGWPKG"
     gene            2156..3424
                     /locus_tag="VF633_06550"
     CDS             2156..3424
                     /locus_tag="VF633_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470753.1"
                     /translation="MLTRRGFGIMAAATSAAASLPAWAQTALTPEEQFFSEGRYLPLY
                     LDLQTKAAAGERAAGGLLSQYASFLGDELTALGREERRRDPAVLLPDLTEAVSRDALE
                     AIVDAAATSRIVILNEAHNTSGHRGFAARVLRALKPLGFDWFAAETFIPPQQDPAPSM
                     RGYRRGMPFVSSLGYYVNDPVYAETVREAARLGYRFADYEERWDQEAADDADSATRIA
                     TRETAEADNLIAAVLASNPDAKVFVYCGYSHAMEKPHTSGTWFAARLKEKTRLDPLTI
                     EQSMNWPATVAENDPPHVAAVLKRFAPTAPISVTLNGQMIASPTYKGQMDISVFHPRL
                     PSISGRPGWLMADPERRAVEVALPPFEGPTLLQAMWSGEGAAGVPADQFLLEPGQTKA
                     TVLLHPGSYFLRFERADGIDAAFGSIDVKA"
     gene            complement(3421..3855)
                     /locus_tag="VF633_06555"
     CDS             complement(3421..3855)
                     /locus_tag="VF633_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8470754.1"
                     /translation="MTKPATHHTGGCLCGAVRYEAEGEPLYAGYCFCTDCRKASGSGF
                     APFLGLAASRVRLTGPVQQHVAIAAHGGPATRNFCPTCGSLVFGGEIGVSDSFTFYAG
                     SLDDPSLFQPTVAIFNADRPAWAPLPEGLEVFEGNPFQGVEL"
     gene            complement(3901..5361)
                     /locus_tag="VF633_06560"
     CDS             complement(3901..5361)
                     /locus_tag="VF633_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8470755.1"
                     /translation="MTHTSLNRRSILSAAGLALPTLALPWGARAQSQPAPPPGPFRFA
                     ARDGQETDAERGFFEVPEDRRVPGSRRIRLGYVRFPSTSAKPGTPIVYLAGGPGGEGS
                     GAATGPRFPIFMALRAVADVIAFDQRGTGLSASIPARPASTGPFPALTHAGMTGWYRD
                     EMQKAWVDWTRAGVAMTGYNTEQSADDINDLRRHLGAEKINLWGISYGTHLALSVLKR
                     HPDAIGRVALASLEGQDQTIKRPARIDAFLGRVDGLLGEDPVVRAAIPDLPALMRRVH
                     DRLEADPATLTVASEAGPVDIKVGGFGVQLLAGGLVANPRTLSMLPGIYLALDAGKTE
                     VLADLLSEPASLFNIRGMPEAMDLASGVSPAKLALVEQEAKTAVLGDAFNFPMPHLLG
                     AIPGVDLGEGFRESIRIDTPALLVAGSLDGRTPLEEQAEVIAQFHNKAQILVENAGHN
                     VFEAHPDVQTLLVKFFREEAVSDTTMSVPPPTFAMG"
     gene            5472..6926
                     /locus_tag="VF633_06565"
     CDS             5472..6926
                     /locus_tag="VF633_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8470756.1"
                     /translation="MWRIFLIAILAVAFPAASNAQEGGQDWQSCRGEPGVGAPTLHDC
                     RQVEGVIDPQGRELWLRSVVQRPAGEEPAALYVVGVASSEVWLNGQQLGENGRPGGSA
                     SAETPGRYEAAFPIPERMWRPADNVVVIHMSSFHGAVRLDSPIAGIVVAPWPWPARTG
                     ALAIAFIAAGALFAAAFGFGVIHSLRRTGSSLTLAALAGVSGLQAILESLRALLPYAY
                     PVHGWRLIGIWVLTAAFAVLLVSYVVSRFWPRARRPLIGLAVVAVAATYLAPGFDLKA
                     VLALMIGIALSAAAAAVAVYRRAPGARLTLIYLAVFMVIGALFPTWLVDLSYFLFAAG
                     LVLPLLMAEVVRLGRGDQQREVALTEAAARPNCLTVVSSKRVERVPIADIVAVVGADD
                     YVELRLTDGRSLLHAARLDRLETELPSTFLRTHRSVIANLAHVSGLERGGGRGRLLMR
                     ASQPLPISRNRLAAVRDALDDPRPLERTRGDANG"
     gene            complement(6928..10050)
                     /gene="putA"
                     /locus_tag="VF633_06570"
     CDS             complement(6928..10050)
                     /gene="putA"
                     /locus_tag="VF633_06570"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX8470757.1"
                     /translation="MNAPFSALALSQNWDTLDHGKFRDEPQAVAANLATLPLEPATRV
                     AVVDEAVALVEHARRSQKKQGVVESFLQEFSLGTREGLALMCLAEALLRTPDEDTRDR
                     LIAEKIGSADWASHLGQSDSLFVNASTWGLMLTGKLVDADEQAKSDLPGFLTRVAGRL
                     GEPVIRQAVAAAVRIMGEQFVVGRTIEAALKRSNKEGWLCSFDMLGEGARTTADAERY
                     EKIYADAITAVGKTSKGQGPEVGHGVSVKLSALSPRYEATHEDRVWTELYPRIHRLAR
                     IAAQYDINFTMDAEEADRLALSLKLLDRLANEADLGDWTGLGLAVQAYQKRGPEVMAR
                     IADLARTSGRRLMVRLVKGAYWDTEIKRAQVSGRTDYPVFTTKAATDLNYLVCAQAMI
                     DAAPHLYSQFATHNAHSLAAVHRMARDKSVKIEFQRLHGMGEALYEAARETFGPMTVR
                     AYAPVGGHEDLLPYLVRRLLENGANSSFVHALLDERVPASAVAADPITTVEAHPDRHA
                     KIPTPKDMYMDRQNSLGRDYSQLADRERHAVALEKVDSEKLTSGPIVGGKLKAGINPT
                     DVINPYDGVTVLGHVSEATEADVDAAAAAAFRAQGPWDRSGGSKRAPVLRAMADALEA
                     DMDRLVALLSREAGKTLNDGVAEVREAADFCRYYAMLAEKDFGGPQTLNGPVGEVNRL
                     VLHGRGVFACISPWNFPLAIFTGQIAAALAAGNAVLAKPAEQTPLIAAEAVRLFHKAG
                     LDADLLSLLPGRGETVGARLVSHPGVDGVAFTGGTDTATMINRSLAARPGAIVPFIAE
                     TGGLNGMFVDTTALREQVIDDVINSAFGSAGQRCSALRLLYVPHDAADSLIEGLKGAL
                     AVQVVGDPADPATDIGPVIDADAKGLLDAHLKRLEGDAKIIARATVPADAKGHVFAPT
                     IAEVPTADYLEREVFGPILHVVRYHPKDLKTVAGKLAARGYGLTLGVHSRIEAFAEEV
                     TRLVPAGNVYINRGVTGAVVGVQPFGGEGLSGTGPKAGGPNSLIRYASEKAISNNISA
                     QGGDPALLNL"
     gene            10168..11253
                     /locus_tag="VF633_06575"
     CDS             10168..11253
                     /locus_tag="VF633_06575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8470758.1"
                     /translation="MRIVLATDAWEPQVNGVVRTLTRTVAECRAMGHEVEVIEPSQFK
                     TIPCPTYPEIRLALGAEEEIRERLRAFEPEAVHIATEGPIGIAMRRICVEWKLPFTTS
                     YHTKFPEYVSARFPIPVQVGYAYMKWFHKPSGRLMVATPTLRDELQAHGFKNVSPWTR
                     GVDTEQFHPDLEPIFDELGGKDWPRPFFLNVGRVAVEKNIEAFLETDLPGTKIVVGDG
                     PARADLEARYPNAKFLGARFGEELARCFRDADVFVFPSWTDTFGLVILEAMAAGTPVA
                     AYPAHGPIDIIPGSNAGAIDEDLKVACLKALECDPKAVRAYAEKFSWRASAEEFVHNL
                     EPYPEPERGRFWRRLRRIARVRRRAAA"
     gene            11445..13229
                     /locus_tag="VF633_06580"
     CDS             11445..13229
                     /locus_tag="VF633_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470759.1"
                     /translation="MTSEWTDHIKMAMETRSRQWRLRVGIAGLIALVFHSFTGGGFAL
                     AWFGVYCALQLIESDFRPGGAWIQRLNPKDWARASIGMILLNNLAFATFGLAQVQDGS
                     GLAISCSAILFGGAILNGVMVSTSSRELVLASIAPHVLFSLVLPIVMVMDGATPLAAL
                     QVFIALAMLVVASLMAWKQLGGTLAEVQQAKGEAVEANKAKSEFLAVMSHEIRTPLNG
                     VLGMAQAMAADTLSGRQRERLEVVAQSGQALLAIVNDVLDLAKVEAGKVELENRPFDL
                     ETTIRQAEAGFEAIALSKGVRFTSTIDESAVGRYVGDALRLRQVLANLISNAVKFTDE
                     GCITVAATRKADLLVLTVEDTGPGCTPEQMGRLFRKFEQADASTTRRFGGTGLGLSIC
                     SELTSLMGGTIEAQSVEPSGLRFVTRLPLEWLGGKDEAVADIATVEAGEEDRQLRVLA
                     AEDHPVNRQVLTVLLAQSAGIVPMIVEDGAQAVQAWRDGPIEGGDWDLILMDVQMPVM
                     DGPTAAAAIRAEEAATGRAATPIIALTANVMTHQIEEYRRMGMDAVVGKPIQMEELLR
                     AMARVLEDRDATTAGTGTDNEPRLSRIA"
     gene            complement(13234..14307)
                     /locus_tag="VF633_06585"
     CDS             complement(13234..14307)
                     /locus_tag="VF633_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8470760.1"
                     /translation="MTRKQLAASAALMAVTVIAVAACGQGSGGGQAQRSGIWAAGSST
                     VFPFATRVAENFGRNNPGGAAPRVESLGTGGGIQAFCQGVGPSTPDIANASRPMKKSE
                     FDLCQKNGVVEIVEIKIGYDGIVIATARTGNAFNFELNDLYEGLAKSVPGAGGAMVAN
                     PATTWNQVNPGLPSQRIQVYGPPPTSGTRDAFLELGMAPGGLLIPAVAAIKGDKDKFE
                     ALTHTLREDGAWVDAGENDNAIVQTLSRTPGSLGVFGFSFLEQNLDTVKPETIDGVAP
                     NADTIADGSYPLARSLYVYIKKAHIGVTPGLQQFVAEFMSDSATGRGGYLQDRGLVPL
                     PEDQLQAQRAIAANLTPMAAPSR"
     gene            complement(14363..15112)
                     /gene="phnE"
                     /locus_tag="VF633_06590"
     CDS             complement(14363..15112)
                     /gene="phnE"
                     /locus_tag="VF633_06590"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX8470761.1"
                     /translation="MALDLLIWGGVAVVLIYSIKAVDLGNISKLFTNSGHTADLAGDL
                     LKPDFSDWKLYVAKMWETIQIALWGTFLAVFAAIPLGLAAARNIAPTWVVVPTRLIMN
                     LLRSVPDLVIGLLFVLAVGLGPLPGVLAIALNTAGVLAKLFSEAVESIDKGPVEGVRA
                     TGASPLHEIFWGVIPQVGPLWTSFALYRFESNSRSATILGVIGAGGIGQVLIDGMNSY
                     SYSAVSAMVIVIVVAVTLIDMLSQAMRKRLL"
BASE COUNT         2497 a         4665 c         5387 g         2672 t
ORIGIN      
        1 gctgctgctg cccgctgcgc tgtcgaccgg ctggcaccgt cgagccgagc ctgcggtgcg
       61 gttcatggtc tgctggttgg tgccggcatg ggtgatcttc gagctcgccc cgaccaagct
      121 gtggcactac acattgccga cctttggggc catcgccctg ctgatggccg ccgccctgac
      181 acgcccgatt ggcatgatca gccgccgtgt aggggccggt ctggcggtca tcgccgccat
      241 ggccatctgc gccatcaccg tctacggcct caccgcattc ggcacctcga cggcccagac
      301 atgggcggcg atgactatcg tctgcgccgc tgtggcgggc gcgggcggcg ccttcatcct
      361 gctgaatcgc gcagccgtct cggcgctggc ggtctcggtc gttttcggcg tcatggctca
      421 cgcggcgttg gccgggacct tgcgccagct gcggcctctg tcggtcgcgc cgatgctggt
      481 cgagaagatg gagcaggcgg gcatcgcgcc agatcagggc ctgatccccg gcccggtcgc
      541 catcaccacc ttccatgagc caagcttcgt cttcctgacc ggcaaggaca ccgaactgac
      601 cgacgcggct ggtgctgcga aggccttggc tgagggccgt ccggcgatcg tcgaggctcg
      661 cgacgccgag gccttccggc aggccatggc cgtaacgggc gcgggcggcc gggccgtcgg
      721 caacgtgcgg ggccacaact attccagcgg cgacgacgtc aacctgacgc tctatgcccc
      781 gaccggagcc cccgctgcgc ccagtgcgat cgccgagggc gaggctgaat gaagaggccg
      841 atccagatcg tggaggtcgg tccgcgcgac ggcctgcaga acgagaaggt cattctggaa
      901 ccgtcgatcc gcgccgaact ggtgcgacgg ctcgaggcgg cgggcgcgcg tcggatcgag
      961 gctgtcagct tcgtccatcc gaaatacgtg ccccagatgg ccggcgccga agaggtcatg
     1021 gccgaactgc cgaaacagcc gggccgcagc cgcatcgggc ttgtgctcaa cggcaagggc
     1081 tatgaccgcg cgctcggcac ggcggtggac gaggtcaatg tggccatgag cgccaccgac
     1141 ggcttcggcc tcaagaacca gggtctgtcg gtggtccagc aggtcgagat gctggccgcc
     1201 atcatcgctg gtagagcgaa cgctgaagga gaactcggcg cgacgcctgc attgtcggcg
     1261 acgatcagtt gcgtctgggg ctgccctttc gacggcgagg tctcgaccga tcaagttcag
     1321 gatctggtcg gccggattgc cgaacttggc gtggccgaga tcggactggc cgacaccata
     1381 ggtgtcggtg acccgtggag cgtaacccgc aaggtcgaag cggcgaggaa ggccgcgccc
     1441 gacgcgaccc tgcgcctgca tttccacgac acgcgcaaca ccggtctggc caacgcctat
     1501 gcggggatcg aggccgggat cgacgtgctc gacgcctcgg tcggcggcat cggcggctgt
     1561 ccgttcgcac cgggctccac gggcaacatc gcgaccgaag acctcgtcta cttgctcgag
     1621 cgcggcggct actcgaccgg ctacgacctc gacgccctga tcgtgaccgc cacgtggatc
     1681 ggcgagaaaa tcggccgccc cgcccccagc gccctcagcc gcgccggagg ctggccgaag
     1741 ggctgattgg gggcgagtgt ctccacgctc gcaaaaatac gaaaagggag actctcgccc
     1801 ctgagcattg cgagggttgg gcgcgcaacc gcagccgtca gacggatatt cgcgaccctg
     1861 tggcgggcgg cgatgagcgg attttcaaag accgggctgg atccgacgac agttcgcggg
     1921 tccagcagcc attgtacccg ccaccgcgac cccgaggcgt cgatttccga gacaccgagc
     1981 aagtgtctcc agtcgggggt cgtgaagggg cgggagtatc agggtcgaga gtctccaccg
     2041 tgtccacgcc ctgtccccca ttagagaacg aagatcaggc atctggcgtg cacgccaaag
     2101 tcacagtctc gccaaggttg cgggatgatc tgcgtgtata aatcgggggc tttcaatgct
     2161 gacaagacgc gggttcggga tcatggcggc ggcgacgagc gcggccgcca gtttgcccgc
     2221 gtgggcgcaa acggctttga cgccggagga acagttcttc agcgagggcc gctatctgcc
     2281 gctctatctt gatctccaga ccaaggccgc agcgggcgaa cgtgcggctg gaggcctcct
     2341 gtcgcaatac gcgtcgttcc tcggggacga gttgacagcc ttgggacgcg aggagcggcg
     2401 gcgtgatccg gcagtgctcc tgcccgatct gacggaagcc gtaagccggg acgcgctgga
     2461 ggcgatcgtg gacgcggccg caacgtcgag gatcgtcatt ctcaacgaag cccacaacac
     2521 gtccgggcat cgcggcttcg ccgcgcgggt tcttcgggct ctgaaacctc ttgggttcga
     2581 ctggtttgcg gcggagacgt ttatcccgcc acagcaagac cccgctccgt ccatgcgggg
     2641 atatcggcga ggtatgccgt tcgtctcatc gctcggctat tacgtaaacg accccgtcta
     2701 tgccgagacc gtgcgagagg cggcgcgtct cggctatcgc ttcgccgact acgaggaacg
     2761 ctgggaccag gaagcggctg acgacgcgga ctccgcgacc cggatcgcga cccgggaaac
     2821 agccgaggcc gacaatctga tcgcggcggt tctggcttcg aaccctgacg ccaaagtctt
     2881 cgtctattgc ggctacagcc atgcgatgga gaagccgcac actagcggca catggtttgc
     2941 ggcgcgtttg aaggaaaaga cgagacttga tcccttgacc atcgagcagt cgatgaactg
     3001 gcccgcgacc gttgccgaga atgatccgcc tcatgttgcg gcggtgctga aacgctttgc
     3061 acccaccgcc ccgatttccg tcactctgaa tggccagatg atcgcgtctc caacatataa
     3121 ggggcagatg gatatttcgg tcttccaccc tcgattgccg tcgatctcag ggcgccccgg
     3181 atggctgatg gcggatcctg agcggcgagc ggttgaggtg gctctgccgc ctttcgaagg
     3241 gccgacgttg ttgcaggcga tgtggtccgg cgaaggcgcg gccggcgttc cagcggatca
     3301 gttcctgctt gaaccgggtc agacaaaggc aacggtgctc ctgcacccag gctcctattt
     3361 tctgcggttc gaacgcgccg acggcatcga cgcggccttc gggtcgatag acgttaaagc
     3421 ctagagctcc acgccttgga acgggttgcc ttcgaacact tcgaggcctt cggggagggg
     3481 cgcccaagcg gggcggtcgg cgttgaagat ggcgacggtg ggctggaaca gcgatgggtc
     3541 gtcgagcgat cccgcgtaga aggtgaagct gtcgctgacg ccgatctctc cgccgaagac
     3601 cagcgagccg caggtcgggc agaagttgcg ggtcgccggt ccgccgtggg ccgcgatggc
     3661 gacatgctgc tggaccgggc cggtcagcct gacccgcgac gccgccagtc cgagaaacgg
     3721 cgcaaagcct gagcccgagg ctttgcggca atccgtgcag aagcaatatc cggcgtagag
     3781 cggctcgccc tcggcctcat aacgcaccgc cccgcagaga cagccgccgg tgtgatgcgt
     3841 cgcgggtttg gtcatgggac gaggttagga ggggcggggt agtcggacaa gccttcctgc
     3901 tcaccccatg gcgaaggtcg gtggcgggac gctcattgtc gtgtcgctta cggcctcctc
     3961 gcggaagaat ttcaccagca gggtctggac gtcggggtgg gcctcgaaga cgttgtgacc
     4021 ggcgttttcg acgaggattt gcgccttgtt gtggaactgg gcgatgacct cggcctgctc
     4081 ttcgagcggc gtgcggccgt cgagggagcc ggcgacgagg agggcggggg tgtcgatccg
     4141 gatggattcg cggaagcctt cgccgagatc gacgccgggg atggcgccca gcaggtgggg
     4201 catggggaag ttgaaggcgt cgccgaggac ggcggtcttg gcctcctgct cgacgagcgc
     4261 cagctttgcg ggcgatacgc ctgatgcgag gtccatcgcc tccggcatgc cgcgaatgtt
     4321 gaacaggctg gccggctccg acagaagatc agccagcacc tcagtctttc ccgcgtcgag
     4381 cgccagatag atgccgggca gcatggagag ggtcctcggg tttgcgacga ggcctccggc
     4441 gaggagctga accccgaagc ccccgacttt gatatcgacg gggcctgctt cactggcgac
     4501 ggtcagggtc gcgggatcag cttcgagcct gtcgtgcacg cgtcgcatca gggcgggcag
     4561 atccgggatg gctgcgcgga cgacggggtc ttcaccgaga agaccgtcga cgcgtccgag
     4621 gaaggcgtcg atgcgcgccg gacgcttgat ggtctgatcc tgaccttcga ggctggccag
     4681 ggcgacgcga ccgattgcat caggatgccg cttcaggacc gagagggcga ggtgggtgcc
     4741 gtagctgatg ccccacagat tgattttctc ggcgcccaga tggcggcgca agtcgttgat
     4801 gtcgtcggcg ctctgttcgg tgttgtagcc ggtcatggcg accccggcgc gggtccagtc
     4861 gacccaggcc ttctgcatct cgtcgcgata ccagccggtc attcccgcgt gggtcagggc
     4921 ggggaaaggg ccggtggagg cgggccgtgc aggaatgctg gcggagaggc ccgtcccgcg
     4981 ctgatcgaag gcgatgacgt cggcgacggc tctcaacgcc ataaagatcg ggaaccgggg
     5041 accggtcgcg gcgcccgagc cctctccgcc cggtccgccg gcgagataga cgatgggcgt
     5101 tcccggcttg gccgaggttg aagggaaccg gacatagccg aggcggatgc ggcgcgatcc
     5161 gggaacgcgc cgatcctcgg gaacctcgaa gaagccgcgt tcggcgtcgg tctcctgacc
     5221 atcccgggcg gcaaagcgga aggggccggg aggcggcgcc ggctgcgatt gcgcgcgcgc
     5281 tccccaaggc agggccagtg tcgggagagc gagtccggcg gcggacagga tggagcggcg
     5341 gttgagcgat gtgtgggtca tgaagcggtt gaagcagagg tttcgacccg gcggcgcgcg
     5401 cttcggcgaa agacgcgcct gcatcggtga acggcagcgc ttttcggcaa acgacacgat
     5461 atgttcgcgc gatgtggcgg atttttctca tcgcgattct ggcggtcgcg ttcccggcgg
     5521 catccaatgc gcaagaaggc gggcaggact ggcagtcgtg tcgcggtgaa cccggcgtgg
     5581 gggcgcccac gcttcacgac tgccgccagg tcgagggcgt catcgatccc caagggcgcg
     5641 agctttggct tcgttcggtg gtgcagcgtc cagccggcga ggaacctgcg gccctgtacg
     5701 tcgtcggcgt cgcttcgtcg gaggtctggc tcaacggtca gcagctgggc gagaatggcc
     5761 gtccgggtgg ctcagcgtcc gccgaaacgc ccggccgcta tgaggctgcg tttcccatcc
     5821 cggagcggat gtggcggccg gcggacaatg tggtggtgat ccacatgtcg tccttccacg
     5881 gggcggtacg tctggacagt cccatcgccg ggatcgtggt cgcaccttgg ccgtggcccg
     5941 cgcgaacggg ggctctggcg atcgccttca tcgcggcggg cgctctgttc gctgcggcgt
     6001 tcggcttcgg tgtgatccat agtttgcgcc gcacgggctc cagcctgacg ctcgcggcgc
     6061 tggccggtgt ttcgggtctg caggcgatat tggagagcct gagggcgctc ctcccctatg
     6121 cctatccggt gcatggctgg cggctgatcg ggatctgggt gctgactgcc gccttcgcgg
     6181 ttctgctggt gtcctatgtc gtgtcgcggt tctggccacg agcgcgccgt ccgttgatcg
     6241 gcctcgccgt ggtggcggtc gccgcgacat atctggcccc cggcttcgat ctgaaagctg
     6301 tgctggcctt gatgatcggt atcgcccttt cggccgcggc ggccgctgtg gctgtctacc
     6361 gccgagcgcc cggcgcgcgc ctgaccctga tctatctcgc tgtcttcatg gtgatcggag
     6421 cgctgttccc gacctggctc gtcgatctgt cctatttcct gttcgccgcc ggtctggttc
     6481 ttccgcttct gatggccgag gtcgtgcgac tggggcgcgg cgatcagcag cgggaggtcg
     6541 cgctgacgga ggccgccgcg cggcccaact gcctgacggt ggtgtcatcg aaacgcgtcg
     6601 agcgcgtgcc gatcgcggac atcgtggccg tggtcggggc ggacgattat gtggagctga
     6661 ggctgacgga cggtcggagt ttgctgcacg ccgcccgtct ggatcggctt gagacggaac
     6721 tgccctcgac cttcctgcga acccaccggt cggtgatcgc caacctcgcc cacgtaagtg
     6781 gattggagcg tggtggcggc cggggccggt tgctgatgcg cgccagtcag cctctgccga
     6841 tcagccgaaa ccggcttgcg gccgtccgcg atgcgctgga cgaccctcgc ccgttggaac
     6901 ggacgagggg cgacgcgaac ggttaaccta caggttcagc agcgccggat cgccgccctg
     6961 ggccgagatg ttgttgctga tggccttttc cgaggcatat cggatcaggc tgttggggcc
     7021 gccggcctta gggccggtac cggacaggcc ttcaccgcca aacggttgca cgccgacgac
     7081 ggcgccggtg acgccgcgat tgatatagac attgccggcg gggacgaggc gggtgacttc
     7141 ctcggcgaag gcctcgatgc gcgagtggac gcccagcgtt aggccgtaac cgcgggcggc
     7201 cagcttgccg gcgaccgttt tcaggtcctt cggatgatag cggacgacgt ggaggatggg
     7261 gccgaagacc tcacgctcca gatagtctgc ggtcggaacc tcggcgatgg tcggggcaaa
     7321 gacgtggcct tttgcatcgg cgggcacggt ggctcgagcg atgatcttcg catcgccttc
     7381 gaggcgtttg aggtgggcgt cgagcaggcc cttggcgtcg gcgtcgatga ccggaccgat
     7441 gtcggtggcg ggatcggcgg gatcgccgac gacctgaacc gccaaggcgc ccttgaggcc
     7501 ttcgatcagg ctgtcggcgg cgtcgtgggg gacatagagc aggcgcaggg ccgagcagcg
     7561 ctggccggct gagccaaagg cggagttgat aacgtcgtcg atgacctgtt cgcgcagggc
     7621 ggtggtgtcg acgaacatgc cgttcaggcc gccggtctcg gcgatgaagg gaacgatggc
     7681 gccgggacga gccgccagcg aacggttgat catggtcgcc gtgtcggtgc cgccggtgaa
     7741 ggcgacgccg tcgacgccgg ggtggctgac cagtctggcg ccgaccgtct cgccacggcc
     7801 gggcaggagg gatagcaggt cggcgtcgag accagcctta tgaaacaggc gcacggcctc
     7861 ggcggcgatc aggggcgtct gttcggcggg cttggcgagg acggcgttac cggcggccag
     7921 agcagcggcg atctgaccgg tgaagatggc gagcgggaag ttccacgggc tgatgcaggc
     7981 gaagacgccg cgaccgtgca ggacgaggcg gttcacctcg ccgaccgggc cattcagggt
     8041 ttgcgggccg ccgaaatcct tctcggccag catggcgtag tagcggcaga aatctgcggc
     8101 ctcgcggacc tcggcgacgc cgtcgttgag ggtcttgccc gcctcgcggc tgagcagggc
     8161 caccagacga tccatgtcgg cctcgagcgc atcggccatg gcgcgcagca ccggcgcgcg
     8221 cttcgagccg cctgagcgat cccatggccc ttgcgcgcgg aaggcggcgg cggcggcggc
     8281 gtcgacatcg gcctcagtcg cttcggagac gtggcccaga acggtgacgc cgtcataggg
     8341 gttgatcacg tcggtggggt tgatcccggc cttgagcttg ccaccgacga tggggccgga
     8401 ggtcagcttt tcgctgtcga ccttttccag agccacggcg tgacgctcgc ggtcggcgag
     8461 ttgcgagtag tcgcgcccga gggagttctg gcggtccatg tacatgtcct tgggcgtggg
     8521 gattttggcg tgacggtcgg ggtgggcctc gacggtcgtg atggggtcgg cggcgacggc
     8581 ggaggcgggc acgcgctcgt ccagcagggc gtggacgaag gaggagttgg cgccgttctc
     8641 cagcagacga cggaccagat agggaagcag gtcctcgtgg ccgccgacgg gggcataggc
     8701 acgcaccgtc atgggaccga aggtttcgcg cgcagcctcg tacagggcct cacccatgcc
     8761 gtggaggcgc tggaactcga tctttaccga cttgtcacgg gccatccggt ggacggcggc
     8821 gagggagtgg gcgttgtggg tggcgaattg cgaatagagg tgcggcgcgg cgtcgatcat
     8881 cgcctgagcg cagacgaggt agttgaggtc ggtcgcggcc ttggtggtga agacggggta
     8941 gtcggtgcgg ccggacacct gggcgcgctt gatctcggtg tcccaatagg cgcccttgac
     9001 cagccggacc atcagacgac ggcccgatgt gcgggcgagg tcggcgatgc gggccatgac
     9061 ctcgggaccg cgcttctgat aggcctgaac ggcgaggccc aagccggtcc agtcgccgag
     9121 atcagcctcg ttggctagcc ggtccagcag cttcagcgac agggcgaggc ggtcggcctc
     9181 ctcggcgtcc atggtgaagt tgatgtcgta ctgggccgcg atcctggcca gccggtggat
     9241 gcgcggatag agctcggtcc agacgcggtc ctcgtgggtc gcctcgtagc gcggcgacag
     9301 ggccgagagc ttgaccgaga cgccatggcc gacttctgga ccttggcctt tcgacgtctt
     9361 gccgacggcg gtgatggcgt cggcgtagat cttttcatag cgttcggcgt cggcggtggt
     9421 gcgggcaccc tcgccgagca tgtcgaacga gcagagccag ccctccttgt tcgagcgttt
     9481 caacgccgcc tcgatggtgc ggccgacgac gaactgctca cccatgatgc gcacggcggc
     9541 agcgacggcc tgacggatga cgggttcacc gaggcgaccg gcgacgcgtg tcaggaagcc
     9601 gggcaggtcg ctcttggcct gttcgtcggc gtcgaccagc ttgccggtca gcatcaggcc
     9661 ccaggtcgag gcgttgacga acaggctgtc ggattgcccg aggtgggacg cccagtcggc
     9721 cgaaccgatc ttctcggcga tcaggcggtc gcgggtgtcc tcgtcgggcg tgcgcaacag
     9781 ggcttcggcg aggcacatca aggccagacc ttcgcgggtg ccgagcgaga actcctgcag
     9841 gaagctctcg acgacgccct gtttcttctg gctgcggcgc gcgtgctcga cgagtgcgac
     9901 ggcctcgtcc acgaccgcga cgcgggtggc gggctccagc ggcaaggtgg ccagattggc
     9961 ggcgaccgct tggggttcgt cacggaattt gccgtggtcg agggtgtccc agttctgcga
    10021 cagggcgagt gcggagaaag gtgcgttcat gggcgcgtct tagcccgttc gcccgtgagt
    10081 gggaaagcct tgccgatccg tctcgccttc ctcgttgtga cccgctatgg cttcctgctc
    10141 gacaacggcg ggagccattc ggcccatatg cgcatcgttc tggccaccga cgcctgggag
    10201 ccccaggtca acggcgtcgt ccgtaccctg acccgcacgg tggcggaatg ccgcgccatg
    10261 ggtcatgagg tcgaggtcat cgagcccagc cagttcaaga ctattccgtg cccgacctat
    10321 ccagagatcc ggctggcgct gggggcggaa gaagagatcc gcgagcgtct gcgggccttt
    10381 gagcccgagg ccgtgcacat cgccaccgaa ggcccgatcg gcatcgccat gcggcgtatc
    10441 tgcgtcgagt ggaaactgcc cttcacgacc agctatcaca cgaaattccc tgaatatgtc
    10501 tcggcgcggt tcccgatccc ggtacaggtc ggctacgcct acatgaagtg gttccacaag
    10561 ccatccggac ggctgatggt ggcgaccccg acgctgcgtg acgagcttca ggcgcacggg
    10621 ttcaagaacg tctcgccgtg gacgcggggc gtcgacaccg aacagttcca tcctgacctc
    10681 gagcccatct tcgacgaact gggcgggaaa gactggcctc ggccgttctt cctgaacgtc
    10741 ggtcgggtgg cggtcgagaa gaatatcgag gccttcctcg aaaccgacct gccggggacc
    10801 aagatcgtcg tcggcgacgg tccggcccgc gccgatctgg aggcgcggta tcccaatgcg
    10861 aaattcctcg gagcccggtt cggcgaggag ctggcgcggt gcttccgtga cgccgacgtg
    10921 ttcgtcttcc cgagctggac ggataccttt ggtctggtga tcctggaagc catggctgcg
    10981 ggaacgccgg tcgcggccta tcctgcgcac gggccgatcg acatcatccc gggctcgaac
    11041 gccggggcta tcgatgagga cttgaaggtc gcctgtctca aggcgctgga atgcgacccg
    11101 aaggcggtgc gcgcctatgc cgagaaattc agctggcgcg cctcggccga agagttcgtg
    11161 cacaatctgg agccctaccc ggagccggaa cgcggccggt tctggcgcag gttgcggcgg
    11221 atcgctcgtg tgcggcgacg cgcagcagcc tgaatgcacc gctccgaggt atggccgtat
    11281 acggattgct acccagcgct gtaaccgggt ccatgccggg aagttgaggg cgtccattct
    11341 cgccagtgtt ttgagatcac acgcgaaaga ttgttgaacg cggttccgcg agtttacgat
    11401 ctgtgaacca aactgcggtg tatttcctga tcgaggaaat actcatgacg tccgaatgga
    11461 ccgaccacat caagatggcg atggagaccc gttcgcggca gtggcggctg cgcgtcggta
    11521 tcgccgggct gatcgctctg gtcttccact ccttcaccgg cgggggcttc gccctggcgt
    11581 ggtttggcgt ctattgcgcc ctgcaactga tcgaatctga tttccgtccg ggtggcgcct
    11641 ggatccagcg gttgaacccc aaggactggg ctcgcgccag tatcgggatg atcctgctca
    11701 acaatctggc ttttgcgacc ttcgggctgg cccaggtcca ggacggatcg ggcctggcga
    11761 tctcgtgctc ggccatcctg ttcggcggcg cgatccttaa cggcgtgatg gtcagcacgt
    11821 cttcacgtga gctggtgctg gcctcgatcg cgcctcatgt actgttctca ctggtcctcc
    11881 ccatcgtcat ggtcatggac ggcgctacgc cgttggccgc cttgcaggtc ttcattgcgc
    11941 tagcgatgct ggtggtcgcc agtttgatgg cctggaagca actgggcggc acattggcgg
    12001 aggtgcagca ggctaagggc gaggcagtcg aggccaacaa ggccaagagc gaatttctcg
    12061 ccgtcatgag tcacgaaatt cgtacgccgt tgaacggagt gctcggcatg gcgcaggcca
    12121 tggctgcgga caccctgtcg ggccggcaac gcgagcggct tgaggtcgtg gcccagtcgg
    12181 gccaggcctt gctggcgatc gtcaacgatg tgctggacct cgccaaggtc gaggcaggca
    12241 aggtcgagct ggagaaccgt ccgtttgatc tggaaaccac gatccgtcag gccgaggccg
    12301 gtttcgaggc gatcgccctg tccaagggcg tcaggttcac ctcgaccatc gatgagagcg
    12361 cagtcggccg gtatgtcggt gacgccctgc gccttcggca agtgctggca aatctgattt
    12421 ccaacgcagt caagttcacc gacgagggct gtatcaccgt cgcggcgacg cggaaggccg
    12481 atcttctggt cctgaccgtt gaggacaccg gcccgggctg cacgcccgag cagatggggc
    12541 gattgttcag gaagttcgaa caggcggacg cctcgaccac gcgccggttc gggggcacgg
    12601 ggctgggcct gtccatctgc agtgagttga ccagtctgat gggcgggacg atcgaggccc
    12661 agtctgtcga accgagcggt ctgcggttcg tcacccggct gccgcttgaa tggctgggtg
    12721 ggaaggacga ggcagttgcc gacatcgcca ctgtggaggc gggggaggaa gaccggcaat
    12781 tgcgggtgct ggcggccgag gatcacccgg tcaatcggca agttttgacg gttctgctgg
    12841 ctcagtcagc cggtatcgtt ccgatgatcg tggaggacgg cgcccaggca gtgcaggcct
    12901 ggcgcgacgg gccaattgag ggcggcgact gggacctgat cctgatggat gtgcagatgc
    12961 cggtgatgga cggaccgacc gcagcggccg ctatccgcgc cgaggaggca gcgacgggcc
    13021 gcgcagctac gccgatcatc gccctgaccg ccaacgtcat gacgcatcag atcgaagaat
    13081 accggcgcat gggcatggac gcggtcgtcg gcaaacccat ccagatggaa gaactgctcc
    13141 gcgccatggc tcgcgttctg gaagaccggg acgccacgac ggccggtacc gggaccgaca
    13201 atgagccccg actgtcccga atagcctgaa ggcttacctg gacggagcgg ccataggcgt
    13261 caggttggcc gcgatggcgc gctgggcctg aagctgatct tccggcagcg gcacgagccc
    13321 acggtcctgc agatacccgc cccgtcccgt cgctgagtcc gacatgaact cggcgacgaa
    13381 ctgctggaga ccaggcgtga cgccgatatg ggccttcttg atatagacgt agaggctgcg
    13441 ggccagcgga tagctgccgt cggcgatggt gtctgcgttc ggcgcaacgc cgtcgatggt
    13501 ctcgggctta accgtgtcga ggttctgctc caggaacgag aagccgaata cgccgagcga
    13561 tccgggcgtg cgcgacaggg tctggacgat ggcattgtcg ttctcgcccg cgtcgaccca
    13621 ggctccgtct tcgcgcagag tatgcgtcag ggcctcgaac ttgtccttgt cgcccttgat
    13681 cgcggcgaca gccgggatca ggagcccgcc cggagccatt ccgagttcga ggaaggcgtc
    13741 gcgcgtgccc gaggttggcg gcgggccata gacctggatg cgctggctcg gcagacccgg
    13801 attgacctgg ttccatgtgg tggcggggtt agcgaccatc gcgccccctg ctccgggcac
    13861 ggacttggcc agaccttcgt agagatcgtt gagctcgaag ttgaaggcgt tgccggtgcg
    13921 ggccgtggcg atgacgatgc cgtcatagcc gatcttgatc tcgacgatct cgacgacgcc
    13981 gttcttctgg cacaggtcga actcggactt cttcatcgga cgcgaggcgt tggcgatgtc
    14041 cggggtggag gggcctacgc cctgacagaa ggcctgaatg ccgccgccag tgcccaacga
    14101 ttccacgcgc ggcgctgcgc cgccggggtt gttgcggccg aaattctcgg cgacgcgggt
    14161 ggcgaagggg aagacggtcg aggagccggc ggcccagatg ccggaacgct gggcctgccc
    14221 gccgccggag ccctgtccgc acgcggccac agcgatgacc gtcacggcca tcagcgcagc
    14281 cgaggcagcg agttgtttgc gggtcatcag acgctctccg gacggacgtt tccgagcccg
    14341 cttagagcag gcttttgtga cgttagagca accgcttgcg catcgcttgc gacagcatgt
    14401 cgatcagggt cacggcgacg acgatgacga tgaccatcgc tgaaacggcg ctgtaggaat
    14461 acgaattcat tccgtcgatc agaacttggc cgatgccccc cgcgccgata accccgagga
    14521 tggtcgcgga ccggctgttg gactcgaacc gataaagcgc aaatgatgtc cacaacggtc
    14581 cgacttgcgg gataacaccc cagaatattt cgtggagggg ggacgctccg gttgcgcgta
    14641 cgccctcgac tggacccttg tcgatcgact ccacagcttc tgagaatagc ttggcgagga
    14701 caccagccgt gttgagcgcg atagccagaa cgccgggcag ggggcctaga ccgaccgcca
    14761 gcacgaacag caggccgatc accaaatcag ggaccgagcg cagaaggttc atgatcaatc
    14821 gagtggggac gacgacccac gtaggcgcga tgttgcgcgc tgccgcaagg cccagcggga
    14881 tcgcagcgaa gaccgcgagg aacgtgcccc acaaggcaat ctggatcgtc tcccacatct
    14941 ttgcgacgta gagtttccag tcggaaaaat caggcttgag cagatccccc gcgagatcgg
    15001 ccgtgtgacc gctattggtg aacagcttgc tgatgttgcc cagatcgacg gccttgatgc
    15061 tgtagatcag cacgacggcg acgccgcccc agatcagcag gtcgagcgcc atggcgccga
    15121 gcggcttgga cggcgccttc gggattgcgg tcagatcgac gggagcggtg gtcaaggctg
    15181 gacctcggcg cggacgcgca ggcgttgcag ttcggcctcg g
//
LOCUS       DASVTM010000240        10428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_447517,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000240 DASVTM010000000
VERSION     DASVTM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447517"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VF633_06595"
     CDS             complement(<1..1054)
                     /locus_tag="VF633_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8470762.1"
                     /translation="MTEGVYRFDRFVLDAGDRRLLDGQTPVEINARYLDALALLVREH
                     GRLLTKDRFLDEVWRGVPVTDEALTQCIRSLRRILGDDATRPRFIETVPKHGYRFIAP
                     LEPVRSQPPFAPAASGRLRRWDPFFREGRAGMIGGGLAGLVGGVIYGFAIVADTGTPG
                     GGAISAVLVIACLTMVIGLIGGAGVGFGVASSLFASTHTRPWAVAGGAGGGMMVGAVA
                     KLVGLDAFTLLFGEAPGHITGAAEGAVLGGAIGLGVWAIDRFSARTTVLLAGLLGITA
                     GALISMGGGRLMGGSLNLLAMQFPGSRIRMEHLGILFGENGWGPVSGAVTAGLEGLLF
                     VSFVVAAVLWDRKRRNS"
     gene            1164..1700
                     /locus_tag="VF633_06600"
     CDS             1164..1700
                     /locus_tag="VF633_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470763.1"
                     /translation="MTSDGGMLGRRLNIAVWCGAVCLWLLPLVAMQFTREVAWTTFDF
                     IVWGLMLVVAAGAFELATRMSGNIAYRLGAGLALAASFFMVWVNLAVGIIGNENEPLN
                     LMFFGVLLIGVLGSVAVMFRARGMIWTMLIMAIAQGVVAVVAQVYGHFIWPISVAFTA
                     VWLTSAWLFKRAAETRPA"
     gene            complement(2028..2174)
                     /locus_tag="VF633_06605"
     CDS             complement(2028..2174)
                     /locus_tag="VF633_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470764.1"
                     /translation="MYALRYCCGRRDRRDLVNASADNPRLITFEDSSTYVRGDPYDRE
                     NGDY"
     gene            complement(2280..2675)
                     /locus_tag="VF633_06610"
     CDS             complement(2280..2675)
                     /locus_tag="VF633_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470765.1"
                     /translation="MMATILIADDDPVLREIASEMLRSTNHAAVLAEDGLEVLRLLDG
                     ITVDVLVTDMLMPNMDGVEVIMAARAKYPALKVLAISGGGSVGASYMLHMAKVLGADA
                     ILEKPLKLDTFIGAIRDLLEDQIVGSKAA"
     gene            complement(2875..3084)
                     /locus_tag="VF633_06615"
     CDS             complement(2875..3084)
                     /locus_tag="VF633_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEX8470766.1"
                     /translation="MQAAAIAAAGITSALARFDASAARTAVAPLDDLAAETVERLEAE
                     AAVTANAAVLRTADNMMGALLDIFV"
     gene            3178..3510
                     /locus_tag="VF633_06620"
     CDS             3178..3510
                     /locus_tag="VF633_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX8470767.1"
                     /translation="MTSQPSQAPAPGERYASFAAFYPYYIHEHSNRTCRRIHVVGTGL
                     VILSWILALTVSLWWFAASPVIGYGFAWVGHFFFEKNRPATFQYPLWSLMGDFRLFFE
                     TVSGRRRF"
     gene            complement(3514..4110)
                     /locus_tag="VF633_06625"
     CDS             complement(3514..4110)
                     /locus_tag="VF633_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX8470768.1"
                     /translation="MRFAAAILVAFTALASSAVGEPVAYEAVVAIERTALETPDLANG
                     SSGDDNTATGAAADLTAEQAELMAGDPDWRAHASLYHAGGGGATGNDSLGCRPVAMRT
                     LATDPRVIPRRTRLFIRETVGMRLADGTVHDGYWYASDTGGAIKGQRVDLYTGNGRGS
                     MQQARGLNQRTLTITRAGTFNGCPPATTRTAPLQMASN"
     gene            4330..4914
                     /locus_tag="VF633_06630"
     CDS             4330..4914
                     /locus_tag="VF633_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8470769.1"
                     /translation="MLTLYGDIRSGNCLKVKWLLDWLKRDYRWIDVDIMTGGSPTPEF
                     LGLNTAGQVPTLVFADGRTLAQSNAILIHLAEQTTLIPVDDHERAVMLEWLFWEQYSH
                     EPYVAVARFQRVYAGKAAEEVEPRLMERGHGALARMEARLRDRDWIAATEPSLADLAL
                     VAYTRVAPEGGFDLDGYPALRRWIGRTEEVFGLV"
     gene            complement(4911..6293)
                     /locus_tag="VF633_06635"
     CDS             complement(4911..6293)
                     /locus_tag="VF633_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8470770.1"
                     /translation="MSKPNLLKNSALIAAAILAFTSTSTHAQDWQVKQEWVAAHEAFL
                     AGDALRGRGSATPDEGVAAAYVGAMFQGYGLSTAPGMTSYLQTAGVTKTTPSGHTTLT
                     VGGVTLTQGQNLTVLLTSGTSQTGALTLADAAASPPAALLIAPTEGQGLGPAIGGAIE
                     GGAKLLILRETDSLKALAARRGPPRPSFALSNEAVSGMSGGPSPDVVVVSVADYDRLK
                     AMAGATATIDPGAVQRLETVTTNAIGFLPGSDPTAGVLLVTSHLDHLGVRPDGTIMHG
                     ANDDASGTAAVLELAHALASGEAHRRGILFVAYGSEEIGGLGSTYFGEHPPVPLTDIV
                     ANLEIEMIGAQDPKMEPGVMMMTGFDRSNFGPTLKEKGALVAPDLYPEQNFFQRSDNY
                     SLALKGIVAHTISGWAVTPTYHSPDDDIAHLDLPFMTRAIQSLIEPLRWMASSDFTPE
                     WAPGGRPEPR"
     gene            6446..7465
                     /locus_tag="VF633_06640"
     CDS             6446..7465
                     /locus_tag="VF633_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 Nuclease"
                     /protein_id="HEX8470771.1"
                     /translation="MKRIALAAVAMLAVAVPAAQVDAWGSTGHRMIGVAAVRALPDSL
                     PAFIRGMDAAAEVGEISREPDRSKGAGQPHDRERDTAHFVDLDDTGHVMTANGLSIDA
                     LPNLKSEYDAQLLAAGIKVDDAGYLPYAIMDGWQQLVRDFAYWRVLSAIEAREVDPAK
                     RAWYREDRLRREALTRRDMGILSHYVGDGSQPLHTSIHFNGWGDYPNPEGFTNSRQTH
                     GTFEGGFVRRNVRLDMVEAAMAAPMLDGFEMKRSIVSYLKTGVGQVVPFYTLEKAGAF
                     AEGDPRGVVFATERVAAGAAELRDLTILAWQASETATIGYPVVKVADVQSGAVDPWAS
                     LYGED"
     gene            7470..8234
                     /locus_tag="VF633_06645"
     CDS             7470..8234
                     /locus_tag="VF633_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8470772.1"
                     /translation="MQPPVVILDRPQMGENIGAVARVMANFGLETLRLVSPRDGWPQE
                     RAWATASGADWVLEGVEVFDSVAAAVADLNTVFATTARPRETRQPVRTPREASRVLYE
                     DRASGLNIGLLFGAERAGLETTDIALCHGIVTIPIDPRHQSLNLAQAVAINAYEWRTL
                     VLDAPPPNFREGDPPASGELQLGMYEHLEKELDAAGFFHPPEKFRSMSQNLRVMLGRA
                     AFSAQEVATFRGVITALSKGRGRVLERLAREKAAKG"
     gene            complement(8292..8576)
                     /locus_tag="VF633_06650"
     CDS             complement(8292..8576)
                     /locus_tag="VF633_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470773.1"
                     /translation="MLKVYCDFNDKTDDEQYWILLYEHRPLNDVVNELGLVDGDRVIL
                     YQDVGDFEIEAVLMFGQTHDYFLGTEICARPDWSTLVRFPATNTAPSTGS"
     gene            complement(8581..9642)
                     /gene="rutA"
                     /locus_tag="VF633_06655"
     CDS             complement(8581..9642)
                     /gene="rutA"
                     /locus_tag="VF633_06655"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HEX8470774.1"
                     /translation="MEVGVFIPINNNGWLISETAPQYMPSFDMNKRIAIKAEEYGLDF
                     LLSMIKLRGFGGKTEFWEYGLESFTLMAGLAAVTEKIKIYATCPTLIIPPAFAARMCN
                     TIDSISHGRFGLNLITGWQKPEYSQMGLWPGEDHFRNRYKMLDEYAHILRDLWETGRS
                     DLKGDYYEMDDCLVRPQPAGDMKIICAGSSDEGLAFSAKWADYAFCLGKGVNTPTAFA
                     FNNERLAEATAKTGRDVKMFVLVMVIAADTDEEAMARWKNYNAGIDIDAISWLKDQGA
                     ADTVNATTNVRQLSAPEGAVNINMGTLVGSYATVAKMLDQMAEVEGTGGVLLTFDDFE
                     RGIEDFGQKIQPLMECRKG"
     gene            complement(9654..>10428)
                     /gene="rutD"
                     /locus_tag="VF633_06660"
     CDS             complement(9654..>10428)
                     /gene="rutD"
                     /locus_tag="VF633_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HEX8470775.1"
                     /translation="YYEIHDGPPGAKTVILSAGLGGSAAFWAPQIDALTSAFRVILYD
                     HRGTGRSDRSLKPDHSVASMAEDIVTVLDATDTPKAHLVGHAAGGNAGLQLALDHPDR
                     LDKLVVVNGWSRPDPHVARCFAARTRLLEAFGPQAYVEAQSLFLYPPDWISENDALLK
                     AQEPHHVASFPRTDVMLARINALLAFDIDDRLADIPHPVLVNASADDMLVPARLALRL
                     AAGLPNATLEIAPWGGHAFTATLPDVFNAQLIAWLSRDT"
BASE COUNT         1800 a         3071 c         3681 g         1876 t
ORIGIN      
        1 ggctatttcg ccgcttgcgg tcccacagga cggcggccac gacaaagctc acgaaaagca
       61 aaccttccaa acccgcagtc accgctccac tgaccggtcc ccagccattt tcgccgaaga
      121 gtatccccag gtgctccatg cgaatacgcg acccgggaaa ttgcatggcc agcaaattca
      181 agcttccacc catcaagcga ccgcccccca tggaaatcaa cgcgcccgcc gttatgccca
      241 acaaacccgc gagcaggacg gtcgtcctgg cgctgaaccg gtcgatcgcc cagacaccga
      301 gaccgatcgc tccgcccagc accgcgccct ccgccgcgcc ggtgatatgg cctggggcct
      361 ctccgaacag cagcgtgaag gcgtcaaggc cgacaagttt tgcgacggcg ccgaccatca
      421 tgccgccgcc ggcgcctcct gcgacggccc atggtcgtgt atgtgtgctg gcgaagagag
      481 aactggcgac gccgaagccg actccagcgc cgcctatcag gccaatcacc attgtcaggc
      541 aggcgatgac cagcacggcg gagatggctc cacccccagg cgttccggtg tcggccacga
      601 ttgcgaagcc atagatcacc ccgccgacga ggccggccag accgccgccg atcatcccgg
      661 cgcggccctc ccggaagaac gggtcccagc gacgaagccg cccggacgca gcaggcgcaa
      721 agggcggttg ggatctgacg ggctccaacg gcgcgatgaa ccggtagccg tgctttggaa
      781 ccgtctcgat aaagcggggg cgagtcgcat cgtcccccag gatgcgccgc aggctgcgga
      841 tgcactgggt cagggcttcg tccgtaacgg gaacgcctcg ccagacctcg tcgaggaacc
      901 tgtccttggt cagcaatcga ccatgttcgc gaaccagaag cgcgagcgcg tccagatagc
      961 gggcgttgat ttcgaccggc gtttgtccat ccagaagccg acgatcaccc gcatcgagaa
     1021 cgaaacgatc gaaacgataa acgccctcag tcacggtttc agcgtttcct cagaacctgc
     1081 tcatgacgct caccgtccgc tggatcagga gaaaagcctc actcatgcag ggatcgaagg
     1141 caatgacaaa gaggtcgaag acgatgacga gcgatggagg gatgctgggg cggcggctca
     1201 atatcgcggt gtggtgcggc gccgtctgcc tgtggctttt gcctctggtc gcgatgcagt
     1261 tcacgcgaga agtggcgtgg acgacgttcg acttcatcgt gtggggcttg atgctggtcg
     1321 tcgccgccgg cgcgttcgag ctggcgacgc ggatgtcggg aaacatcgcc tatcggctgg
     1381 gcgccggact ggccctcgca gcgtccttct tcatggtatg ggtgaacctc gccgtcggga
     1441 tcatcggcaa cgagaatgag ccgctgaacc tgatgttttt cggtgttctg ctgattggcg
     1501 tgctgggatc ggtggctgtg atgtttcgag cccggggtat gatctggacg atgctgatca
     1561 tggccatcgc gcaaggtgtg gttgcggtgg ttgcgcaggt ctatggacat ttcatctggc
     1621 cgatcagcgt ggcgttcacg gcggtctggc tgacgtcggc atggctgttc aaacgggcgg
     1681 ccgagacccg gccggcctga cggtctggca acaggccgag gtggcctcgg gcttgcgtga
     1741 tggaccgttt gagtcccgtc acgggacgat gagggaccta tggatctttg tgagttcgcc
     1801 gttcatgtgc ggccggcagg cgatcactgg tcgtgggtgg tgctcgacga agatggagtg
     1861 gccgcccgta acgggacggc ccggaattct gacgaagccc ggaaggcggc gatcagcctg
     1921 gcggaaagac tcacccgttg tcgcatggtc acaaagggcg gctggtgatg ttgggcgatg
     1981 tgctcgcgac gctccatcga gcacggaagg gaatcttcct cacggcgtca atagtcgccg
     2041 ttctcgcgat cgtagggatc accgcgcacg taggttgaac tgtcttcgaa cgtgatcagc
     2101 cggggattgt cggcggatgc gttgaccaga tcccggcgat ctcgccgacc acagcaatag
     2161 cgaagggcgt acaccaactc acgccggcag cgagaaaatg gtcaacgttg ccggcggggc
     2221 tttagacaac cgatcccgag ccggcgcctt ggcgaaactt caggaggacc agtccgggtc
     2281 taggcggcct ttgaaccaac gatctggtct tcgagcaggt cgcggatggc tccgatgaag
     2341 gtatccagct tcaggggctt ctcgaggatt gcatcggcgc cgaggacctt cgccatgtgc
     2401 aacatgtagg atgcaccgac cgagcctccg ccgctgatag ccagaacctt cagcgcagga
     2461 tatttcgccc gcgcggccat gatcacctcg acgccatcca tgttgggcat cagcatgtcc
     2521 gtcacgagca catcgacggt gatcccgtcc agcagcctga gaacctccag cccatcctcc
     2581 gcgaggactg ctgcatggtt ggtagagcga agcatctcgg atgcaatctc tctaaggaca
     2641 gggtcgtcgt ctgcgatcag gatcgttgcc atcatctacc tccgaagcgg accgtcgcac
     2701 ctgagggcta ccctgccgtg aacggagatc attaacatct gaaagaccag cgcttcggcg
     2761 agaaagcgat gcgagtttgg tctgcttcga tgacgccgga ggcggcgggg tgacagctgt
     2821 gaccagaaac ggggagcgct tgaagcctct tccgccaagc ggaaaggcgc ggctctagac
     2881 gaagatatcc agcagcgcgc ccatcatgtt gtccgcagtc cgtagtacgg cggcgttggc
     2941 cgtcactgcg gcctcggcct caaggcgttc gacggtttcg gccgcgaggt cgtcaagggg
     3001 cgcaacggct gtgcgagcgg cgctggcatc gaagcgagcg agagcgctgg tgatcccggc
     3061 ggcggcgatg gcggcggctt gcatgggttc aggataggcg aacgcgctta agcggccgtg
     3121 cgcgagcgtg gtgaatgggc gattgcatcg ggcggggcgg gagcgcagat tcgcgggatg
     3181 accagtcaac cctcacaggc gccggcgccg ggcgaacgct acgcctcctt cgcggccttc
     3241 tatccctact atatccacga gcattcgaac cggacgtgcc ggcggatcca tgtggtgggg
     3301 acggggctcg tgatcctgag ctggatcctg gcgctgacgg tcagtctttg gtggttcgca
     3361 gccagtcctg tcatcggcta cggtttcgcc tgggtcgggc acttcttctt cgagaagaac
     3421 cggccggcga cgttccagta tccgctgtgg agcctgatgg gcgacttccg cctgttcttc
     3481 gagaccgtca gcggccgccg caggttctag cggctagttc gaggccatct gcagcggggc
     3541 cgtccgggtg gtggcgggag gacagccgtt gaaggtaccg gcgcgggtga tggtcagggt
     3601 gcgttggttg aggccgcggg cctgctgcat cgagccacgt ccgtttccgg tgtagaggtc
     3661 gacgcgctgg cccttgatcg cgccgccggt gtcggaggcg taccagtagc cgtcgtggac
     3721 ggtgccgtcg gccagacgca tgccgaccgt ctcgcggatg aacaggcggg tgcggcgcgg
     3781 gatgacgcgc gggtcggtgg cgagggtccg catggcgacc gggcggcagc cgaggctgtc
     3841 gttccccgtc gctccgccac caccagcgtg atagaggctg gcgtgggcgc gccagtcggg
     3901 atctccggcc atcagctcgg cctgttcggc cgtcagatca gccgctgccc ccgtcgctgt
     3961 gttatcgtcg ccgctggaac cattcgccaa atccggggtt tccaaagcag tccgctcgat
     4021 ggcgacgacg gcttcgtaag cgacgggctc acccactgcc gaggaggcga gtgcggtgaa
     4081 ggcgacgagg attgcggcgg caaaacgcac aggtagaact cacgacggcg ccgaccgggg
     4141 accgtggtgg caggtgctat ctcgttcgca cgtatggcca gaatgtggcg gttgctttgg
     4201 ccgcgtgact ggcagcgcgc tccggatgga tcaatccacc atcggtagaa tgattctcaa
     4261 aggagcttgg gcataccgag agattgcgat atcagtatag ggcacttcca aatccttaga
     4321 ctggtcgcga tgcttactct ttatggcgat attcgttcgg gcaactgctt gaaggtcaag
     4381 tggttgctgg actggctcaa gcgtgattat cgatggatcg acgtcgatat catgacgggt
     4441 ggtagcccga caccggaatt cctcggcctg aacaccgccg ggcaggtccc gacactggta
     4501 ttcgcggatg ggcggacact ggcgcaatcg aatgcgattt tgatccatct ggccgagcag
     4561 acgacgctca ttcctgtgga tgaccacgaa cgggcggtga tgctggagtg gctgttctgg
     4621 gagcagtaca gccatgagcc ctatgtcgcg gtggcgcggt tccagcgtgt ctacgccggg
     4681 aaagcggcag aggaggtcga gccgcggttg atggaacggg gtcacggcgc gctggcccgg
     4741 atggaggcgc ggctccggga tcgcgattgg atagcagcca cggagccgtc gttggcggac
     4801 ctcgcattgg tcgcctacac gcgtgtcgcg ccggagggcg ggttcgatct ggacggttac
     4861 ccggcgctca gacgctggat aggccgaacc gaagaggtgt tcgggctcgt ttaacgaggc
     4921 tcgggccgcc cgccgggcgc ccattcgggg gtgaagtcgc tggaggccat ccagcgcagg
     4981 ggctcgatca gggactggat cgcgcgggtc ataaacggca ggtccagatg ggcgatgtcg
     5041 tcgtccgggc tgtggtaggt cggggtgacg gcccagcccg agatggtgtg ggcgacgatg
     5101 cccttgagcg cgagggagta gttgtccgac cgctggaaga aattctgttc cgggtagagg
     5161 tcgggcgcga cgagagcgcc cttttcctta agcgtcgggc cgaagttcga gcggtcgaaa
     5221 ccggtcatca tcatcacgcc gggctccatc ttcggatcct gagcgccgat catctcgatc
     5281 tcgagattgg cgacgatgtc ggtcaagggc acgggcgggt gctcgccgaa atacgtcgag
     5341 ccgaggccgc cgatctcctc ggagccatag gcgacgaaca ggatgccgcg ccggtgggcc
     5401 tcaccggatg cgagggcgtg ggccagttcg aggaccgccg ccgtgcctga cgcgtcgtcg
     5461 ttggcgccat gcatgatcgt gccgtcgggg cgaacaccga gatggtcgag gtgggaggtg
     5521 accagaagca cgcctgcggt cgggtcgctg ccgggcagga agccgatggc gttggtggtg
     5581 acggtctcaa ggcgttggac ggcgccggga tcgatggtgg cggtggcgcc ggccatggct
     5641 ttcagccggt cgtagtcggc gacggagacc acgaccacgt ctggcgatgg tccacctgac
     5701 ataccgctga cggcctcgtt gctgagggcg aaggaggggc gcggcgggcc gcgacgggcg
     5761 gcgagcgcct tgagcgagtc ggtctcgcgc aggatcagga gtttggcgcc gccctcgatc
     5821 gcgccgccga tagcgggtcc gaggccctgt ccttccgtgg gggcgatcag cagtgcggcg
     5881 gggggagatg cggcggcgtc cgctagcgtt agcgcgcccg tctgcgacgt tcccgaggtg
     5941 aggaggacgg tcaggttctg gccttgcgtc agggtcacgc cgccgacggt cagggtggta
     6001 tggccgctgg gcgtggtctt cgtcacgcct gcggtctgca gatagctggt catgcccggc
     6061 gcagtgctca ggccgtagcc ctggaacatg gcgccgacat aagcggcggc gacgccttcg
     6121 tcgggagtgg cgctaccgcg accgcgcagg gcgtcaccgg cgaggaaggc ctcgtgtgca
     6181 gcgacccatt cctgtttgac ttgccagtcc tgggcgtggg tcgaggtcga ggtgaaggcc
     6241 aggatcgcag cggcgatcaa ggcgctgttc ttcagcaggt ttggcttcga catcgatggc
     6301 tctccggacg acagggggca tgggtaggca tcgcggctcg cgagcgtcaa gcggcggtcg
     6361 attaaggttc ggcaagaatc cgctcgacga cagcgcccgc gagttttaac cgccaaccca
     6421 gcctcatcct ctcgcgagtt cctccatgaa acgcatcgcc cttgccgccg tcgccatgct
     6481 ggcggtcgcg gttcctgccg cccaggtcga cgcctgggga tcgaccggtc accggatgat
     6541 cggcgtggcg gcggtgcgcg ccttgcccga cagcctgccg gcattcatcc ggggcatgga
     6601 cgccgccgcc gaggtgggcg agatctcgcg agagccggac cggtcaaagg gcgcaggcca
     6661 gccgcacgac cgcgaacgcg acaccgccca tttcgtcgat ctggacgaca ccggccacgt
     6721 catgacggcc aacggcctgt cgatcgacgc cctgccgaac ctgaaatccg agtatgacgc
     6781 gcagctgttg gcggcgggca tcaaggtcga tgacgccggt tacctgccct atgcgatcat
     6841 ggacggatgg cagcagctgg tgcgcgactt cgcctactgg cgcgtgctca gcgccattga
     6901 agcgcgcgaa gtcgatccgg ccaaacgcgc ctggtatcgt gaagaccgtc ttcgccgcga
     6961 agctctgacg cggcgtgata tgggcatctt gtcccactat gtcggcgacg gttcgcagcc
     7021 tctgcacacc tcgatccact tcaacggctg gggcgactat ccgaaccccg aaggcttcac
     7081 caactcgcgc cagacgcacg gcacgttcga gggcgggttc gttcgccgca acgtccggct
     7141 ggacatggtc gaagcggcga tggccgcgcc gatgctggat ggtttcgaga tgaagcgcag
     7201 catcgtcagc tatctgaaga ccggcgtcgg tcaggtggtg cccttctaca cgctggagaa
     7261 ggcgggcgcc tttgctgaag gcgacccgcg cggcgtggtc tttgcgaccg agcgtgtggc
     7321 ggcgggagcg gcggaactcc gcgacctgac catcctcgcg tggcaggcct cggagacggc
     7381 cacgatcggc tacccggtgg tcaaggtggc ggacgtccag agcggcgctg tcgatccgtg
     7441 ggcgtccctg tacggcgagg actagggtcc tgcagccgcc tgtcgtcatt ctcgatcgcc
     7501 cccagatggg cgagaacatc ggcgcggtgg cgcgggtgat ggccaacttt gggctggaga
     7561 cgctgcggct ggtcagtccg cgcgacggct ggccacagga acgggcgtgg gcgacggcgt
     7621 cgggggccga ctgggtgctg gagggtgtcg aggtgtttga cagcgtggct gccgccgtcg
     7681 ccgacctgaa caccgtcttc gccacgacgg cccggcctcg ggaaacccgc cagccggtgc
     7741 ggacgccgcg cgaggcgtcg cgggtgctgt atgaagaccg ggcgtcaggg ctgaatatcg
     7801 gcctgttgtt cggggctgag cgggcggggc ttgagaccac cgacatcgcc ctgtgccacg
     7861 gcatcgtcac catccccatc gacccgaggc accagtcgtt gaacctggcg caggcggtgg
     7921 cgatcaatgc ctatgagtgg cggacgctgg ttctggatgc tccgccgccg aacttccgcg
     7981 agggcgatcc gccggcgtcg ggcgagttgc agctcgggat gtacgagcat ctggagaagg
     8041 aactggacgc ggcaggcttc ttccatccgc cggagaaatt ccgctcgatg agccagaacc
     8101 tgcgcgtgat gctgggccgg gccgcgttct cggcgcagga ggtggcgacg ttccgggggg
     8161 tgattacggc gctgtcgaag ggccgggggc gggtgctgga gcggctggcg agagagaagg
     8221 cggcgaaggg ctgaaacctt ccctatcgag gatcgcgcga gctatgggct gtttctgggc
     8281 ggcaggtcta atcaggaacc ggttgaaggt gctgtgttcg tggctgggaa gcgcaccaga
     8341 gttgaccagt ccggtcgcgc gcagatctcg gtgcccagaa aataatcgtg ggtctggcca
     8401 aacatcagca cggcttcgat ctcgaagtca ccgacgtcct gatagaggat caccctgtct
     8461 ccatcgacca gcccgagttc gttgacgacg tcattcagtg gacggtgttc gtagagcaaa
     8521 atccagtact gctcgtcgtc ggtcttgtcg ttaaaatcgc agtagacctt cagcatgagg
     8581 tcaccccttc cgacactcca tcagcggctg gatcttctgg ccaaagtcct cgatgccgcg
     8641 ttcgaagtcg tcgaaggtga ggaggacgcc gccggtgcct tcgacttcgg ccatctggtc
     8701 cagcatcttc gcgacggtgg cgtaggagcc gacgagggtg cccatgttga tgttcacggc
     8761 gccttcgggg gcggagagct ggcggacatt ggtggtggcg ttgacggtgt cggcggcacc
     8821 ctgatccttg agccaactga tggcgtcgat gtcgatgccg gcgttgtagt tcttccagcg
     8881 cgccatcgcc tcttcgtcgg tgtcggcggc gatgaccatg acgaggacga acatcttcac
     8941 gtcgcggccg gtcttggcgg tcgcctcggc cagacgctcg ttgttgaaag cgaaggccgt
     9001 cggggtgttg acgcccttgc cgaggcagaa ggcgtagtcc gcccatttcg ccgagaaggc
     9061 gagaccttcg tcggacgagc ctgcgcagat gatcttcatg tcgccggcgg gctggggacg
     9121 aactaggcag tcgtccatct cgtagtagtc gcccttgagg tccgagcggc cggtttccca
     9181 aaggtcgcgc aggatatggg cgtattcgtc gagcatcttg tagcggttgc ggaagtgatc
     9241 ctcgccgggc cacaggccca tctgggaata ttcgggcttc tgccagccgg tgatcaggtt
     9301 caggccgaag cgaccatggc taatggagtc gatcgtgttg cacatccggg cggcgaaggc
     9361 gggcgggatg atcagggtcg ggcaggtcgc gtagatcttg atcttctcgg tgacggcggc
     9421 gaggccggcc atcagggtga agctctccag accgtattcc cagaactcgg tcttgccgcc
     9481 gaaaccgcgc agcttgatca tagaaagcag gaaatcgagg ccgtattcct cggccttgat
     9541 ggcgatgcgc ttgttcatgt cgaacgaggg catgtactgc ggcgcggtct ccgaaatcag
     9601 ccagccgttg ttgttgatcg ggatgaagac gccgacttcc atggtgcaat ccttcaggta
     9661 tcgcgcgaaa gccaggcgat cagttgggca ttgaagacat ccggaagcgt cgccgtaaag
     9721 gcatgaccgc cccatggcgc gatctcgagc gtcgcgttcg gcagtccggc ggcgagacgc
     9781 agcgccagac gagcggggac cagcatgtcg tcggcggagg cgttgacgag gacggggtgg
     9841 ggaatgtcgg ccagccggtc gtcgatatcg aaggccagca gggcgttgat gcgggccaac
     9901 atgacgtctg tgcgggggaa gctggcgacg tggtgcggct cttgcgcctt gagcagggcg
     9961 tcgttctcgc tgatccagtc gggtggatac aggaacaggc tctgggcctc gacataggcc
    10021 tgcgggccaa aggcttcgag caggcgggtg cgggcggcga agcagcgggc gacgtgcgga
    10081 tcggggcggg accagccgtt gacgacgacc agcttgtcga ggcggtcggg atggtcgagc
    10141 gcgagctgga gcccggcgtt gccgcctgct gcgtggccga cgaggtgggc tttgggcgtg
    10201 tccgtggcgt cgaggacggt gacgatatcc tcagccatag aggcgacgga gtggtcgggc
    10261 ttgaggctcc ggtcgctgcg gcctgtaccg cgatggtcgt agaggatgac gcggaaggca
    10321 ctggtcaggg cgtcgatctg tggggcccag aaggcggctg aaccgccgag accggcggag
    10381 aggatgaccg tcttggcgcc gggagggccg tcatggattt cgtaatag
//
LOCUS       DASVTM010000241         6519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_608698,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000241 DASVTM010000000
VERSION     DASVTM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608698"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1216
                     /gene="ruvB"
                     /locus_tag="VF633_06665"
     CDS             182..1216
                     /gene="ruvB"
                     /locus_tag="VF633_06665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX8470776.1"
                     /translation="MTDDRLIAPEAAPGETYDRALRPQTLSEFVGQEQAKGNLKVFID
                     AARARGEALDHVLLFGPPGLGKTTLAQIVAKELGVGFRTTSGPILAKAGDLAAILTNL
                     EPRDVLFIDEIHRLAPQVEEILYPAMEDHVLDLIIGEGPSARSVRIDLAPFTLVGATT
                     RAGLLATPLRDRFGIPLRLEFYTPDELVRVITGAARKMGVSIDEAGAREIGSRARGTP
                     RVAGRLLRRVRDFAEAEGAKVVTKMVAARALARLEIDEAGLDSNDRRFLKALIENYGG
                     GPVGMDTLAAAIAEARDAVEDVIEPYLLQQGFIQRTPRGRMACAKAYAHLGLNEPPKI
                     PTVGPGDLFE"
     gene            1213..2121
                     /locus_tag="VF633_06670"
     CDS             1213..2121
                     /locus_tag="VF633_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX8470777.1"
                     /translation="MNSLWLRRAQSTGAVIAQAVVTAVVGLFAIRLAGAPNSAPIVQS
                     AALGLGALIAMGLALNPWRPGPKGAATLIGVALILVFATLGDESAGVHRWIAVGPVVL
                     QPASIVLPFVVWAVAVTRTNWWGGALTGAFALVLGIQPDAASATALLLALAALAFVRR
                     KVTAPDATALLMALAATVWAWTRVDPLPAVAYVERVVPTAFAANPVTGIAAGLVLFLL
                     PLPFLLRVIAARAQGRTEDHAVALALCGIWIGLILANLFGNYPAPVVGYGASLVIGWL
                     ASLGLCLQRPRPSQASPGWTISPRVR"
     gene            2174..2554
                     /locus_tag="VF633_06675"
     CDS             2174..2554
                     /locus_tag="VF633_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470778.1"
                     /translation="MTRRLNVQRVIGGLGVVVLGLGALSLWAISHGARPGFSQEQASD
                     RVLRQAHEQLGPRAEIRLLQTGRGRVVCGYLAADSRSSPSGFVSRPNRLLLSNDPLKG
                     EFDQMVAQDCPAFPAPPRTTQAPG"
     gene            2551..3000
                     /locus_tag="VF633_06680"
     CDS             2551..3000
                     /locus_tag="VF633_06680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX8470779.1"
                     /translation="MNDQPTAGRFEGRDHLLPIRVYYEDTDFTGLVYHANYVRYLERG
                     RSDFMRLTAKGHAELLDDAEPMAFVVSELNLKYLKPARIDDALVVRTTYDAVKGPRLI
                     ISQTVERDGEVLCRAEVTAVCIHLDGRPRRPTKALVESVTPWLKPAN"
     gene            3139..3909
                     /gene="tolQ"
                     /locus_tag="VF633_06685"
     CDS             3139..3909
                     /gene="tolQ"
                     /locus_tag="VF633_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX8470780.1"
                     /translation="MTVPAAATAVVDAGMLNPVTLFMTADWVVKIVMAGLAVASIWSW
                     AVIIDKAFRFTALNTQAEAFEKALSSGRSLEDVAAEAGPQPTHALPRMLVTALADWRE
                     TRARGQLTEQQGNLLISRIDRSLDSIIAREGQRVENGLGVLSVVATASPFIGLFGTVW
                     GIMNAFGRIASAGNTNLTTVAPAISEALFATAVGLAAAIPAYIAYNKFSIDAGKFSGR
                     LESFADDLQAAVARRLGAPNTPPPPAPTPSEFSIRRAD"
     gene            3911..4369
                     /gene="tolR"
                     /locus_tag="VF633_06690"
     CDS             3911..4369
                     /gene="tolR"
                     /locus_tag="VF633_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX8470781.1"
                     /translation="MAMGGGAGQGHVRGRRRARKRPLSEINVTPLVDVMLVLLIIFMI
                     SAPLLTVGVPVELPKTEASAVPTDNTPITLSIDQNGAIFLDKTPTEYDALAQSVAEAV
                     GDAKKDEKPIFVRADGRAPYQAVARVMARLSANGFTKLNLITDTAPDSEG"
     gene            4371..5240
                     /locus_tag="VF633_06695"
     CDS             4371..5240
                     /locus_tag="VF633_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470782.1"
                     /translation="MNRPSPAILGSIVLHVGVLALAFISWPKKDLPVPMPSSVPVSIM
                     SSEVIEAAAADNPSEELVTEDGASAPTEAVEAPPEPTPSPPTPAPPTPRVPPKVTPPA
                     PAPRPTPTPTPRPTPPRPTPTPPRPTPPRATPTPARPTPPRARPPAATPRRNEPTLDL
                     GALQGPRRPSPNPGRPSTGQQGAGRAPQASGPQLAALGRQVTPNWNVRVVCDLPNGNA
                     VTIRVRVRLDAGGRIQGTPAIEGSSSDPAWRAAADSLMQALRATSPFDVPDGFTAQEV
                     PFRFDTARPCGNR"
     gene            5356..>6519
                     /gene="tolB"
                     /locus_tag="VF633_06700"
     CDS             5356..>6519
                     /gene="tolB"
                     /locus_tag="VF633_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX8470783.1"
                     /translation="MRLKLLLATVAAVAVSTPLLVQGPARAQVPAATAPTQDVVVDEG
                     VLKPFQIAIAPFSGPNGSELSGVLSGDLSRSGYFEAINPRSFIETGLTLANAPNFPQW
                     TQIGAQAVLYGSVTARPDNRNDYGFRLYDPYRQCQLVSYNFTATPDQWRRVAHKIADV
                     VYQRMTGESGFFDTRIIYVAESGTELNRLSRLAIVDQDGYNPVFLTGGEEIILTPRFS
                     SNPDEITYMALGKDYSRIYLYNLSTGRRESLGEFDGQALAPRYSPDGSKVAFSIIRGG
                     NTDVYVMDLRSRQLSRLTSDPGIDTSPSFSPDARQIVFNSDRSGTPRLYVMSADGSGQ
                     RPISRGGGSYTAPSWSPRGDMIAFTKSSGGRFSIGVMNTDGSGERSLSSSYFEE"
BASE COUNT         1044 a         2261 c         2101 g         1113 t
ORIGIN      
        1 aggcttcggg gttgagccgc gagcgcgaca tggcctatcc ctcctgcttt ctcaaacgga
       61 gtcctgtctg ccatgaacaa gaaactgcag gtcgtgctcg ttttcctcgt cggcatcgta
      121 gccatggtcg ttggttacgc cgtattgaaa atgtaggtca gacagggtcg ggagcggttc
      181 gatgacggat gatcgtctga tcgctcccga agccgccccc ggcgaaacct acgatcgcgc
      241 gctgcgaccg cagaccctgt ccgagttcgt cgggcaggag caggccaagg gcaatctcaa
      301 ggtcttcatc gacgccgccc gtgcgcgtgg cgaggcgctg gaccacgtgc tgctgttcgg
      361 acctccgggg ctcggcaaga ccacgctggc ccagatcgtt gccaaggaac tcggcgtcgg
      421 ttttcgcacg acctcgggcc cgatcctggc caaggcgggt gacctcgccg ccatcctgac
      481 gaacctcgaa ccgcgcgacg tgctgttcat cgacgagatc caccggctgg ccccgcaggt
      541 ggaggaaatc ctctatccgg cgatggagga tcatgtgctg gacctcatca tcggcgaggg
      601 accgtcggcc cggtcggtgc gcatcgatct ggcgcccttc acgctggtgg gggcgacgac
      661 acgggccggg cttctggcca cgccgctgcg cgaccggttc ggcatcccgc tgcggctgga
      721 gttctacacc cccgacgagc tggtgcgtgt catcaccggc gcggcccgca agatgggcgt
      781 cagcatcgac gaggccggag cgcgcgagat cggctcccga gcacggggca cgcctcgtgt
      841 cgccggacgg ttgctacgcc gggtccgtga tttcgccgag gccgagggcg cgaaagtcgt
      901 caccaaaatg gtcgcggcgc gggctctggc ccggctggag atcgacgagg caggactgga
      961 ttccaacgac cgccgcttcc tcaaggccct gatcgagaac tacggcggcg gaccggtcgg
     1021 catggatacc ctggccgccg ccatcgcgga ggcgcgcgat gcggtcgagg acgtgatcga
     1081 gccctatctg ctgcaacagg gcttcatcca gcgcaccccg cgaggccgga tggcctgcgc
     1141 caaggcctac gcccacctcg gcctcaacga gccgccgaag atcccgacgg tcggcccggg
     1201 cgacctgttc gagtgaacag tctctggctc cgccgggcgc agtcgaccgg cgcggtgatc
     1261 gcacaggcgg tcgtcacagc ggtggtcgga ctgtttgcca tcaggctggc cggtgcgccg
     1321 aactccgcgc cgatcgtgca gtctgcggcg ctggggttgg gcgcgctgat tgctatgggg
     1381 ctggccctca atccgtggcg acccggcccc aagggtgcgg cgaccttgat cggcgtggcg
     1441 ctgatcctcg tcttcgccac gctcggagac gaaagcgcag gtgtgcatcg ctggatcgcc
     1501 gtcggtcccg tcgtcttgca gcctgcatcc atcgtcctgc ctttcgtcgt ctgggcggtc
     1561 gcggttacga ggaccaactg gtggggcggc gcgcttaccg gcgccttcgc tctggtgctc
     1621 ggcatccagc ccgacgcagc ctcggcgacg gcgctgctgc tggcgctcgc ggccctggcg
     1681 ttcgtaagac gcaaggtcac cgcgcccgac gccaccgccc tgctcatggc gctggcggcg
     1741 acggtgtggg cgtggactcg cgtcgatccg ttgcctgccg ttgcctatgt cgagcgagtc
     1801 gtgccgacgg cgttcgccgc caaccccgtc acaggcatcg ccgccgggtt ggtgctgttc
     1861 ttgttgccgc tgcctttcct gctgagggta atcgccgcca gagcacaggg gcgcaccgag
     1921 gaccacgccg tcgccctggc gctctgtggc atctggatcg ggctgatcct cgccaatctg
     1981 ttcggcaact atccggcgcc cgtcgtcgga tacggcgctt cgctggtcat cggctggctg
     2041 gcgtcgctgg gtctctgcct tcagcgacca cgaccctcgc aggcgtcacc aggctggacc
     2101 ataagtccgc gcgtgcgata gcggccttcc cctgagcggc gaagcccgtc atcctcccat
     2161 cgcaggaggc cgaatgacgc gacgcctgaa tgttcaaaga gtgatcgggg ggctgggcgt
     2221 agtcgtgctc gggttgggcg cgttgagcct gtgggccatc tcgcacggcg cgcgaccggg
     2281 attcagtcag gagcaggcct ccgaccgggt gctgcgtcag gcgcacgagc aactggggcc
     2341 gcgcgctgaa atccgcctgt tgcagacggg ccggggcagg gtggtttgcg gctatctggc
     2401 cgccgattcg cgatcgtctc catctggctt cgtctcacgc ccgaaccggc tcttgctgag
     2461 taacgacccg ctcaagggcg agttcgacca gatggtggct caggactgcc cggcgtttcc
     2521 ggccccgcct cgaacgactc aggcgcccgg atgaacgatc aaccgaccgc cggtcgtttc
     2581 gaaggccgcg atcacctgct gccgatccgg gtctattacg aggacacgga cttcaccggt
     2641 ctggtctatc atgccaacta tgttcgctat ctggagcggg gacggtccga cttcatgcgg
     2701 ctgacggcca agggtcatgc cgagttgctg gacgacgccg agccgatggc cttcgtggtc
     2761 tcggagctga acctgaaata cctcaagcca gcgcggatcg acgacgcgct tgtcgtccgc
     2821 accacctatg acgcagtgaa gggcccccgg ctgatcatat cccagaccgt cgagcgcgac
     2881 ggcgaggtgc tttgccgggc tgaggtgact gccgtctgta tccatctcga cggacgaccc
     2941 cgccgtccga ctaaagctct ggtcgaaagc gttacgccct ggctgaagcc ggcgaactaa
     3001 tgcccgtcct cacgcaccgt tgaggggagc gttagtgacg ttggcccgct cagccgctag
     3061 aggcggggga cgccacacaa tggtcactcc cgcccgctcg atagggtgcg ataccgatct
     3121 tcgaggaccc gcctgccgat gacagtccca gccgccgcaa ctgccgtcgt cgacgccgga
     3181 atgttgaacc ccgtcaccct gttcatgacc gccgactggg tcgtgaagat cgtgatggcc
     3241 ggtctcgctg tcgcatcgat ctggtcttgg gccgtcatca tcgacaaggc tttccgcttc
     3301 acggcgctta acacccaggc cgaggcgttc gagaaggcgc tgagctccgg gcgttcactc
     3361 gaggatgtcg ctgccgaggc ggggccacag ccgacccacg ccctgccgcg catgcttgtc
     3421 acggccctcg cggattggcg cgagaccaga gcccgcggac agctcaccga gcaacagggc
     3481 aatctgctga tcagccgcat cgaccggtcg ctggactcga tcatcgcgcg tgaagggcag
     3541 agggtcgaga acggtctggg cgtgctgtcg gtggtcgcca ccgcctcgcc cttcatcggc
     3601 ctgttcggca ccgtctgggg catcatgaac gcgttcggcc gcatcgcctc ggccggcaac
     3661 accaacctga cgacggttgc gccggcgatc tcggaggccc tgttcgccac ggccgttggt
     3721 ctggcggcgg ccatcccggc ctatatcgcc tacaacaaat tctcgatcga cgcgggcaag
     3781 ttctcgggcc gcctggagag cttcgccgac gacctgcagg ctgccgtagc tcgtcgtctg
     3841 ggcgcgccga acacgccgcc accgcccgca ccgacgccgt ccgaattctc catccgccgg
     3901 gccgactgac atggccatgg gcggaggggc cggacagggc cacgtacgcg gacgacgccg
     3961 cgccagaaag cggccgctga gcgagatcaa cgtcacgccg ctggtcgacg tcatgctggt
     4021 gctgctgatc atcttcatga tctcggcgcc cctgctgacg gtcggggtgc cggtggaact
     4081 gccgaagacc gaggccagcg cggtgccgac cgacaacacg ccgatcacgc tgtcgatcga
     4141 ccagaacggc gccattttcc tcgacaagac gccgaccgaa tacgacgcct tggcacagag
     4201 cgtagccgag gcggtgggcg atgcgaagaa ggacgaaaag ccgatcttcg tgcgtgccga
     4261 cggtcgcgca ccctatcagg cggtggcacg ggtcatggct cgactttcgg ccaatggctt
     4321 caccaagctc aacctgatca ccgacacggc gccggattct gaaggctgac gtgaacaggc
     4381 cgagccccgc catactggga tcgatcgtgc tgcacgtcgg cgtgctggcg ctcgccttca
     4441 tctcgtggcc gaagaaggac ctgcccgttc cgatgccgtc gtcggtgccg gtgtcgatca
     4501 tgtcgtccga ggtgatcgag gctgcggccg ccgacaatcc ctccgaggaa ctggtcaccg
     4561 aggacggcgc cagcgcgccg accgaggccg tcgaggctcc gcctgaacca acgccgagcc
     4621 ctccgacgcc cgcaccgccg acgccgcgcg tgccgccgaa ggtgacccca ccggcccccg
     4681 ctccgcgtcc cacgcccacg ccgacacctc gtccaacgcc gccacggccg accccgaccc
     4741 cgccgcgtcc gacacctcca cgcgcgacgc ccactccagc ccgccccacg ccaccccgcg
     4801 ccagaccccc ggctgcgact ccgcgtcgca atgagccgac tctggatctc ggcgcgcttc
     4861 aggggcctcg ccgtccttcg cccaatcccg gacggccttc gacaggccag caaggcgccg
     4921 gacgagcgcc tcaggcttca gggccgcaac tggctgcact cggtcggcag gtgaccccga
     4981 actggaacgt gcgggtggtc tgcgacctgc ccaacggcaa cgcggtgacg atccgcgttc
     5041 gggtgcgtct ggacgctggc ggacggatcc agggtacgcc ggcaattgaa ggatccagca
     5101 gcgatccggc atggcgcgct gccgccgaca gcctgatgca ggccttgcgc gcgacctccc
     5161 cgttcgacgt ccctgacggg ttcacggcgc aggaagtacc gttccggttc gataccgcac
     5221 ggccctgcgg caaccgctga tcctcccggc tgttcacgca tcgcctaacg gtcgccccat
     5281 tcaccggcaa gctggccacg ggggcgacgg acgcgctaga tcaggcacgc cgccagagta
     5341 tcgagggagc tatccatgcg tctgaaactt ctgttggcca cggttgccgc tgtggcggta
     5401 tcgacgccgt tgctggtcca ggggccggcg cgtgcccagg tccctgcagc gaccgcacca
     5461 acccaggacg tcgtcgtcga cgaaggtgtt ctcaagccct tccagatcgc gatcgcgccg
     5521 ttcagcggac ccaacgggtc tgagctttcg ggcgtcctga gcggcgatct cagtcgctcg
     5581 ggctatttcg aggccatcaa tccgcgcagc ttcatcgaaa ccgggctgac tctcgcaaat
     5641 gctcccaatt ttccgcagtg gacccagatc ggggcccagg cggtgcttta cggctccgtg
     5701 acggcgcggc ctgacaaccg caatgactat gggtttcgcc tgtacgatcc atatcggcag
     5761 tgccagttgg tcagctacaa tttcaccgcc acgccggatc agtggcgccg ggtcgcgcac
     5821 aagatcgccg acgtcgtcta tcagcgcatg acgggtgaga gcggcttctt cgacacccgc
     5881 atcatctatg tcgccgagag cggcaccgag ttgaaccggc tctcgcgatt ggccatcgtc
     5941 gatcaggacg gctacaatcc cgtcttcctg accggcggcg aggagatcat cctgacgccg
     6001 cgcttctcgt ccaacccgga cgagatcacc tatatggcgc tcggcaagga ctacagccgc
     6061 atctacctct acaacctgtc gaccggccgc cgcgaaagcc tcggcgagtt cgacggtcag
     6121 gcgctggcgc cgcgctattc acccgacggc tccaaggtcg ccttctcgat catccgtggc
     6181 ggcaacaccg acgtctatgt gatggatctg cgcagccgcc agctcagccg cctgacctcc
     6241 gatcccggca tcgacacctc gccgtcgttc agccccgacg cgcgccagat cgtgttcaac
     6301 tcggaccgct cgggtacgcc gcgcctctat gtcatgagcg ccgatggctc cgggcagcgt
     6361 ccgatctcgc gcggcggtgg cagctatacg gccccgtcct ggagccctcg cggcgacatg
     6421 atcgccttca ccaagtcatc gggaggccgg ttctcgatcg gcgtcatgaa cacagacggt
     6481 tcgggcgagc gcagcctctc ttccagctat ttcgaggaa
//
LOCUS       DASVTM010000242         7185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_183921,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000242 DASVTM010000000
VERSION     DASVTM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183921"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1214)
                     /locus_tag="VF633_06705"
     CDS             complement(219..1214)
                     /locus_tag="VF633_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX8470784.1"
                     /translation="MPADSSHSTAAQTESMTERARGVIQLNIEALQALERTIDGSLAR
                     ACDVILSRPGYVVVTGMGKSGHIGGKIAATLASTGTNAFFVHPAEMSHGDLGMLRHDT
                     TLLAISASGESRELRDPLIFCQRNDIPVIGMTQRGSSFLARMSAVPLIMPNVAEACPN
                     GLAPTTSTLMTLALGDALAMVLMDRREFTATDFGMHHPGGSLGMSLQSVREWMGDNHA
                     PPASVPLDADFSEVVSAITAGRKGAVAVLNPDQTLAGIITDGDVRRAFTQDIAVIRAA
                     DIMNHHPKVVSPEQRMSDVVDLLSANKIATLFVVENDRPVSIVHIAELMQAGYVS"
     gene            1463..2239
                     /gene="otsB"
                     /locus_tag="VF633_06710"
     CDS             1463..2239
                     /gene="otsB"
                     /locus_tag="VF633_06710"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX8470785.1"
                     /translation="MTLDAGSAPEALPPPPVNLRGLALFLDMDGVLASLARTPDGVLP
                     DPRRTAVLRRLGRALHGRLAVISGRTLAEIDRITESSSPAASGIHGLEIRTGDGAVHT
                     PAAHPGVRAVVAAFRAFAEAHPGVIVEDKGVAAGLHYRQAPDAAAAAEHLARTLAAET
                     GLALQPGHMIFELKTPGTDKGTALTTLMTEPPFTGALPVMFGDDQTDEHAFRAATALG
                     GFGVLVGPMRVTHARYRLDAVPQVLDWLETVAELREEAEA"
     gene            2236..4038
                     /locus_tag="VF633_06715"
     CDS             2236..4038
                     /locus_tag="VF633_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8470786.1"
                     /translation="MNDLPPARPSLDLAPVGNCSISALIDRHGRYIWACAPRVDGDPV
                     FSALMNGETPEHGFWAVELEGLKHVAQEYIRNTPVLRTVLTAEDGASVEIIDFAPRHH
                     KHSRTYRPLAFGRIVRPLKGAPRVTVRLRPSADWGARRAETTNGSNHIRYLCSDVTLR
                     LTTDCPVSHILEERTFRLEQPLTFFLGPDEGYDQEVGPGVASALDRTVAYWKDWVRTL
                     YIPLDYQDAVIRAAITLKLCAYEETGAIVAAMTTSVPEFPESGRNWDYRYCWVRDAYY
                     TVRALNRLGAVDILENYLVYLRNLVDQSAGGHVQPVYGVGLEEDIDERIVTSVAGYRG
                     MEPVRVGNQAREHLQHDVYGQIVLPLVQSFYDTRLLRPGTVDDFDALEKVGDRAFAMH
                     DQVDAGLWEFRTIARVHTYSSVMCWAACDRLAKAAHHLHLTDRAQVWTERAQIIRERI
                     EKETYIPEEGRFGASFGGTELDASLLQLVDLGFLDPMDPRQVATFNAIEKDLKKGPYL
                     FRYVIPDDFGEPETAFNFCTFWFIEALHQNGRDEEAREIFEQMLSRRTHAGLLSEDIS
                     LGDDELWGNYPQTYSLVGIINCAILLSRSWTDVR"
     gene            4119..5564
                     /locus_tag="VF633_06720"
     CDS             4119..5564
                     /locus_tag="VF633_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX8470787.1"
                     /translation="MSRLIVVSNRVSAPSDPAAGSAGGLAMALSAALMKYDGLWFGWS
                     GETVEHFTGELKMEDRAGVTVALVDLEAQDVDEYYNGYANKTLWPLFHHRVDLTAYER
                     TYGEGYERVNGRFAEVLAPLIQPDDIIWIHDYHLIPMARDLRRLGITNRIGFFLHTPW
                     PVRQLLVTLPHHRRLVESLFAYDLLGFQTEEWLDLFRDYVVSEARGELVGDGGLECFG
                     RTVQAGVFPIGIDVDGFIQARNSPQGNRTYDRMAASAAFRSMMVGVDRLDYSKGLEER
                     MLGYEQFLHDNPENRGDVFLVQVTPISRDDVDTYQDIRARLDALAGRINGEYADMDWQ
                     PIRYLNRGYRRDQLAGIYRAARVGLVTPLRDGMNLVAKEYVAAQNPDDPGVLILSRFA
                     GAAEQMGEALLVNPFSREEVADAIKRALTMPIEERIRKWTALMKVVRDTDVATWRDDY
                     VSCLTAIPVSGDGDAPFHEDPFHHEGHADAA"
     gene            complement(5567..6184)
                     /locus_tag="VF633_06725"
     CDS             complement(5567..6184)
                     /locus_tag="VF633_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8470788.1"
                     /translation="MLTLFHAPLSRSSRLIWLMEELGADYEICYCDIARRDGSGGRDR
                     ANPHPDGKVPALIHDGALISECAAVALYLTDLHPEAGMGVSVGHRDRGEYLTWLAWAA
                     GEMEPVLGQRLGGYVPTDPYSVRRYEAVVDRLLDRLWSAPYLMGDRFSAADVMIGATL
                     MWARAHLPESPTLDSYVARISERPARRAAMEKDGDIAVASSSRAA"
     gene            6377..7144
                     /locus_tag="VF633_06730"
     CDS             6377..7144
                     /locus_tag="VF633_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX8470789.1"
                     /translation="MRRADRLFQIVQVLRGSSHPVTAEALATELETSKRSVYRDVAAL
                     MSQRVPIRGEAGIGYVLDSGFDMPPLMLTSDEIEAAVLGAQWVASRGDLALARAARDL
                     VAKIAASVPERLRPVVLEPSLTTGPSWAEPKIDSLDMAQVRVWIRSGRKLVLDYRDEQ
                     DRPSLRTIWPFLIGYRETVRLLIAWCETRQDFRTFRTDRVVEATFLEDRYPARPAQLR
                     LRWQAKIDEERKRYQAENSAFDAVEVVEPSVSLPPTN"
BASE COUNT         1277 a         2401 c         2240 g         1267 t
ORIGIN      
        1 gggatcgacc gctaccaatt ggtcgtgctt gctgcgaacc gggatcatat tgatgtccgg
       61 tcgcgcgatc agccactcgg cgtaggcgag gcagacacga tccacacccg tcggagaact
      121 gcgctccgca cgactgacca accggctggc gtcgaaaagg atatcctgag ccatgtcctg
      181 attacgcctc gactgcgtcc ttgtaagaca cagccccctt acgacacata gcctgcctgc
      241 atcagttcgg cgatatgaac gatcgaaacc gggcgatcgt tctcgaccac gaacagggtg
      301 gcgatcttgt tggccgagag caggtcgacg acgtcgctca tccgctgctc ggggctgacc
      361 accttggggt gatgattcat gatgtcggcg gcgcggatga ccgcgatgtc ctgcgtgaag
      421 gcccggcgga cgtcgccatc ggtgatgatg cctgccagtg tctgatccgg gttgagcacg
      481 gcgaccgcgc ccttgcgacc ggcggtgatg gccgagacca cctcggagaa gtcggcgtcg
      541 agaggcacgc tggctggcgg cgcgtggttg tcgcccatcc attcgcgcac gctctgcagg
      601 ctcatgccca gagacccgcc cgggtgatgc atgccgaagt cggtggccgt aaactcgcgc
      661 cggtccatca gcaccatggc cagggcgtcg ccgagcgcca gcgtcatcag tgtcgaggtc
      721 gtgggcgcca gaccattggg gcaggcctcg gccacgttgg gcatgatcag cggcacggcg
      781 gacattcttg ccagaaagct cgacccgcgc tgggtcatgc cgatcaccgg gatatcgttg
      841 cgctggcaga agatcagcgg atcacgcagt tcgcggcttt cgcccgaggc ggagatcgcc
      901 agcagcgtcg tatcgtgacg cagcatgccg agatccccgt ggctcatctc cgccgggtgg
      961 acgaagaagg cgttggtgcc ggtcgaggcc agggtcgcgg cgatcttgcc gccgatgtgg
     1021 ccggacttgc ccataccggt gaccacgaca tagccggggc gcgacaggat gacgtcacaa
     1081 gcccgggcga gcgagccgtc gatggtccgt tccagcgcct gcagcgcctc gatgttgagc
     1141 tggatcactc cgcgcgcccg ctccgtcatc gactcggtct gcgcggcagt ggaatgggac
     1201 gaatctgcgg gcatcgggat atccttgagc gtaacgcgtt gccggaggcg ggcctgcggc
     1261 ctcgtcgtcg gcgaagcgta gcttcgcgat ctggtgcagg aattgcccca atatcgtccc
     1321 cggcacaatg ctagggcaaa ggcgcaccac cacagcgtcg cttccgtgtt cgccgatttt
     1381 cgacaaaccg gcggtcgctt cccaaccttt gccgtcatga cgcgttactg cgtctggggc
     1441 gaacacgaca atcggagatc caatgacgtt agacgccgga agcgcgcccg aagcccttcc
     1501 acctccgccc gtgaatttgc gcggtctggc gttgtttctg gatatggacg gggtcctcgc
     1561 atccctggcc cgaacgccgg atggggtgct gcccgatccg cgtcgcacag cggtcctgcg
     1621 ccgtctcgga cgggccctcc acggtcggct ggcggtgatc agcggccgca ccctggccga
     1681 gatcgaccgc atcactgaat cgtcgtcgcc agcggcgtca ggcattcacg gactcgaaat
     1741 tcgcaccggc gacggcgctg tccatacccc ggcggcgcac ccgggcgtgc gcgcggtcgt
     1801 cgcagccttc agggctttcg cagaagctca tcccggcgtg atcgtcgagg acaagggcgt
     1861 cgctgccggg ctccattacc gtcaggcccc cgacgccgcc gctgcggccg agcatctcgc
     1921 gcgcacgcta gcggcggaga ccggcctcgc cctgcagccc gggcatatga tcttcgagct
     1981 caagacgccc ggcactgaca agggcacggc gctgacgacc ctgatgacag aaccgccgtt
     2041 cacgggagcc ctccccgtca tgttcggcga cgaccagacc gatgagcacg ccttccgggc
     2101 cgcgaccgcc ctcggcggtt tcggtgtgct ggtcggaccc atgcgtgtga cccatgcccg
     2161 gtatcgtctc gacgctgtgc cccaggttct ggactggctg gagactgttg ccgaactacg
     2221 tgaagaggct gaggcatgaa cgatcttcct cctgcccgcc cctcactcga ccttgctccc
     2281 gtcggcaact gttcgatcag cgccctgatc gaccgccacg gccgatacat ctgggcctgc
     2341 gctccccgcg tcgacggcga tcctgtcttc tcggccctga tgaatggcga gacgcccgaa
     2401 cacggattct gggccgtcga actcgaaggg ctcaaacatg tcgctcagga gtacatccgc
     2461 aacacccctg tcctgcgcac ggtgctgacg gccgaggacg gcgcgtcggt tgagatcatc
     2521 gacttcgccc cgcgccacca caagcattcg cgcacctatc gcccgctcgc cttcggccgg
     2581 atcgtccgtc ccctgaaggg agcaccgcgc gtcaccgtgc ggttgaggcc atccgccgac
     2641 tggggcgcac gccgggccga gacgaccaac ggctccaacc atatccggta cctgtgctcg
     2701 gacgtgaccc tgcgcctgac caccgactgc cccgtttccc atattctgga ggaacggacc
     2761 ttcaggctgg aacagccgct gaccttcttc ctcggccccg acgagggcta cgatcaggaa
     2821 gtcggcccgg gcgtcgcctc ggcgctggac cgcaccgtcg cgtactggaa ggactgggtc
     2881 cgcaccctct acatcccgct cgattatcag gacgcggtga tcagggccgc gatcaccctc
     2941 aagctctgcg cctatgaaga gaccggggcc atcgtcgcgg cgatgaccac gtcggtgccg
     3001 gagttccccg agagcggtcg caactgggac taccgctact gctgggtccg cgacgcctat
     3061 tataccgtcc gcgccctcaa ccgtctgggc gccgtcgaca tcctcgagaa ctacctcgtg
     3121 tatctcagga acctcgtcga tcaatcggcg ggcggccacg tccagccggt ctatggcgtg
     3181 gggctggaag aggacatcga cgagcgcatc gtcacctctg tcgcaggcta tcgcggcatg
     3241 gagcccgttc gcgtcggcaa tcaggcgcgc gagcatcttc agcatgacgt ctacggacag
     3301 atcgtcctgc ctctggtgca gagcttctac gacacccgcc tgctgcggcc cgggacggtc
     3361 gacgacttcg acgcgctgga aaaggtcggt gaccgggcct tcgccatgca cgatcaggtg
     3421 gacgcggggt tgtgggagtt ccgcaccatc gcccgggtgc acacctattc ctcggtgatg
     3481 tgctgggccg cctgcgaccg gctggccaag gccgcccacc atctgcatct gacagatcga
     3541 gcccaggtct ggaccgaacg cgcccagatc atccgtgaac ggatcgagaa ggaaacctac
     3601 attcccgagg aaggtcgttt cggggccagc ttcggcggga ccgagctcga tgcatccctt
     3661 ctgcagctcg tcgacctagg gttcctcgac ccgatggacc cgcgtcaggt ggccaccttc
     3721 aacgccatcg aaaaggacct gaagaaaggc ccctatctgt tccgctacgt catccccgac
     3781 gatttcggcg aacccgagac cgcgttcaac ttctgcacct tctggttcat cgaggccctg
     3841 catcagaacg gccgcgatga ggaggcgcgc gaaatcttcg aacagatgct gtcacgacga
     3901 acccacgcgg gcttgctgtc cgaagacatc tcgcttggcg atgacgagct gtggggcaat
     3961 tatccgcaga cctactctct cgtcggcatc atcaactgcg ccatcctgct cagccgttcg
     4021 tggacggacg ttcggtaaaa caaggcaaaa atcgtcaacc cccaccgcat cacgatgttg
     4081 cgttgcatcc ctactcaagc ttcggcgtta agtcggctat gtcacgtcta attgttgtgt
     4141 ccaaccgggt atcggctccc tccgacccgg ccgccggctc ggccggtggc ctcgccatgg
     4201 ccctctcagc ggcgctgatg aagtatgatg gcctctggtt cggctggtcc ggagagaccg
     4261 tcgaacactt caccggggaa ctaaagatgg aggaccgggc cggcgtcacc gtcgccctcg
     4321 tcgatctgga agctcaggac gtcgacgaat attacaacgg ctacgccaac aagacgctct
     4381 ggcccctgtt ccaccaccgt gtcgacctga cggcctacga acggacctat ggcgagggct
     4441 acgagcgcgt gaacggccgg ttcgccgaag ttctcgcgcc actgatccag ccggacgaca
     4501 tcatctggat ccacgactat cacctgattc cgatggcgcg ggatctgcgc cgcctgggca
     4561 tcacgaaccg gattggcttc ttcctgcaca cgccctggcc ggtgcggcag ttgctggtga
     4621 ccctccccca tcacaggcgg ctggtggagt ccctgttcgc ctacgacctc ctgggcttcc
     4681 agactgagga gtggctcgac ctgttccgcg actatgtcgt ctccgaagca cgcggtgagc
     4741 ttgtcgggga tggtgggctc gagtgtttcg gccgcacggt gcaggcgggc gtcttcccga
     4801 tcggcattga tgttgatggc ttcatccagg cgcggaactc acctcagggc aatcggacct
     4861 acgaccgtat ggcggcttcc gcggctttcc ggtcgatgat ggtgggtgtg gaccggctcg
     4921 actactccaa gggtctcgag gaacgcatgc tcggttacga gcagttcctg cacgacaacc
     4981 cggaaaaccg gggcgacgtc ttcctggtgc aggtaacgcc gatctcgcgc gacgatgtcg
     5041 acacctatca ggacatccgg gcccgcctgg acgcccttgc cggacgtatt aacggcgaat
     5101 acgcagatat ggactggcag ccgatccggt acctgaaccg gggttatcgt cgcgatcagc
     5161 tggccggcat ctatcgcgcc gcccgcgtcg gtctggtcac ccccctgcgc gacggcatga
     5221 acctcgtggc caaggaatat gtcgcggccc agaacccgga cgatccgggc gtcctcatcc
     5281 tgtcacgctt tgccggcgcc gcagaacaga tgggggaggc cctgctggtc aatccgttca
     5341 gtcgtgaaga agtagccgac gccatcaaga gggccctgac catgcctata gaggaacgaa
     5401 tccggaaatg gaccgccctg atgaaggtgg tgcgcgacac cgatgtggcg acctggcgtg
     5461 acgattacgt cagctgtctg acggcgatac cggtcagcgg cgacggtgat gcgcccttcc
     5521 atgaggaccc gttccaccac gaaggccacg ccgacgcggc ctgaactcag gcggcccggg
     5581 acgagctggc gacagcaatg tcgccgtcct tctccatcgc cgctcgccgc gcgggacgct
     5641 ctgaaatacg ggcgacatag gagtccagcg tggggctttc aggcagatgg gcgcgcgccc
     5701 acatcagcgt cgcccctatc atgacgtcag cggcggagaa cctgtcgccc atcagatacg
     5761 gcgcgctcca cagccggtcg agcagacgat cgacgaccgc ctcgtaacgg cggaccgaat
     5821 agggatcggt ggggacatat ccgccgagac gctggcccag cacaggttcc atctcgcccg
     5881 ccgcccaggc gagccacgtc aggtattcac cccggtcgcg atggccgacg gagaccccca
     5941 tcccggcttc aggatggagg tcggtcagat acagcgccac cgctgcgcat tcgctgatca
     6001 gggcgccgtc atggatcaac gcgggaacct tgccgtccgg atgcggattg gcgcgatcac
     6061 ggccgccgga accatccctc cgcgcgatgt cgcagtagca aatctcgtag tcggccccca
     6121 gctcctccat caaccagatc aggcgcgagg accgcgaaag cggggcatgg aacagcgtca
     6181 gcatcgaaaa atccctctcc agtttaaggc gccaagccgg gctcgcggaa cctcgctgcg
     6241 tcagcacgga accggcccat ccaccggccc cccttgacta ctgccaccct gctgacagca
     6301 tgttgtcagc agtgacggtt cagaagcccg atgaggggct aacgccgtgt ccgatctcat
     6361 cacccgggaa agaatcatga gacgcgccga ccggctgttc cagatcgttc aggttctgcg
     6421 cggcagttcg caccctgtca cggccgaggc gctcgcgacc gagctcgaga cgtccaaacg
     6481 cagcgtctat cgcgacgtcg ccgccctgat gagccagcgg gtgcccatta ggggcgaagc
     6541 cgggattggc tatgtgctgg attcgggctt cgacatgcca cccctgatgc taacctcgga
     6601 cgagatcgag gcagcagttc tgggggcgca gtgggtcgcg agccgtggcg acctggcgct
     6661 ggcccgcgcc gcccgggacc ttgtggccaa gatcgccgcc agcgtgcctg aacggctcag
     6721 accggtggtg ttggagcctt cgctgacgac cggaccgagc tgggccgagc ccaagatcga
     6781 cagtctggac atggcccagg tccgcgtctg gatccgctcg ggccgcaagc tggtgctgga
     6841 ttatcgcgac gagcaggacc ggccgtcgct gcgcaccatc tggccgttcc tgatcggcta
     6901 tcgcgagacg gtgcggcttc tgatcgcctg gtgcgaaacc cgtcaggatt tccgcacctt
     6961 ccgtactgac cgagtggtgg aggccacctt cctcgaagac cgctatccgg cccgccctgc
     7021 ccagctcagg ttgcgctggc aggcgaaaat cgacgaggag cgaaagcggt atcaggctga
     7081 aaattcagca ttcgatgcag tggaagtcgt tgagccctcg gtttcactgc cgccaaccaa
     7141 ctgatcccgc tgaaccttct atccctgcag cccattcgcc acagc
//
LOCUS       DASVTM010000243         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_661552,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000243 DASVTM010000000
VERSION     DASVTM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661552"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /gene="mdoH"
                     /locus_tag="VF633_06735"
     CDS             <1..1119
                     /gene="mdoH"
                     /locus_tag="VF633_06735"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX8470790.1"
                     /translation="TIVKAATPFARVSQFGVRLYGRVAAAGLAWWTGAEGSYWGHNAI
                     LRTRAFAEACGLPILPGKKPFGGHIMSHDVVEAALLRRAGWAVHVTAALDGSCEETPP
                     TITDFIRREHRWCQGNLQHIALLGSRGLNGASKMQLFMGVLAYVASPLWFASLVAGLV
                     IQLQYPIDWSSFFYFLHPQFTPFMLASMLAGVLLIGPKIMGAVLVLSRPRETRAFGGS
                     KQIIKGMAAEIFLSATLAPILMVANTVSVTKILCGQDAGWHAQQRDADGITWADAFRS
                     MRWQMITGLGFCAALAFRPDLASFFVPIVGPLLLAAPIAVWTSRRTTGETLARKGFLI
                     TPAGDEQSATPSVLHGPTRPNPMTDGAKVRALRVLQNA"
     gene            complement(1260..2189)
                     /gene="gcvA"
                     /locus_tag="VF633_06740"
     CDS             complement(1260..2189)
                     /gene="gcvA"
                     /locus_tag="VF633_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEX8470791.1"
                     /translation="MARPLLPLNALRAFESAARNLNFSRAADELAVTPGAVSQQIRLL
                     EDIVGGPLFKREAKGLQLTDLGRGAVPLLREGFERLMDASSLLREPPRKKQVSISVAP
                     SFASKWLMPRMDDFHAVHPEIEVWISADMEPATLGEGGADLAVRYGPGDYPGHVVERL
                     MLETVLPVAAPSLMNGKHPIRTPADLVHHTLLHDMSEDGDPSRPDWAMWLKARRVKHP
                     DPRRGPRFNQASMVIEGALAGRGIALAKRTLAQADLSAGRLVAPFADGSEAVGFAYHV
                     VLPRDRPASPSAVAFVDWLKQQAVHHDNSMGQL"
BASE COUNT          373 a          808 c          741 g          424 t
ORIGIN      
        1 accatcgtca aggcggccac cccgttcgcc cgcgtgtcgc agttcggtgt gcgcctgtac
       61 ggccgcgtcg ccgccgctgg cctggcctgg tggaccggcg cggagggctc ctactggggc
      121 cataacgcga tcctgcgcac ccgcgccttc gccgaggctt gcggcctgcc gatcctgccg
      181 ggcaagaagc ctttcggcgg ccacatcatg agccacgatg tcgtcgaggc cgccctgctg
      241 cgccgcgccg gctgggccgt ccacgtcacc gccgctctgg acggttcgtg cgaagagacc
      301 ccgccgacga tcaccgactt cattcgccgc gagcaccgct ggtgccaggg caacctgcag
      361 cacatcgccc tgctcggctc gcgtggtctg aacggcgcca gcaagatgca gctgttcatg
      421 ggcgttctgg cctatgtcgc ttccccgctg tggttcgcct cgctggtggc cggtctggtg
      481 atccagctgc aatatcccat cgactggtcg tccttcttct acttcctgca cccgcagttc
      541 acccccttca tgctggcttc gatgctggct ggcgtacttc tgatcggtcc caagatcatg
      601 ggcgcggttc tggtcctgtc gcgtccgcgt gaaacccgcg ccttcggcgg cagcaagcag
      661 atcatcaagg gcatggcggc cgagatcttc ctttcagcga cgctggctcc catcctgatg
      721 gtcgccaaca cggtttcggt gaccaagatt ctgtgcggcc aggacgccgg ctggcacgcc
      781 caacaacgcg acgccgacgg catcacctgg gccgacgcct tccgctcgat gcgctggcag
      841 atgatcacgg gtctcggctt ctgcgccgcc ctcgccttcc gtcccgacct cgcgtcgttc
      901 ttcgtgccga tcgtcggtcc gctgcttctg gccgctccga tcgcggtctg gacctcgcgt
      961 cgcacgaccg gagagacttt ggcgcggaag ggcttcctga tcacgccggc cggtgatgag
     1021 cagagcgcca cgccttcggt cctgcatggt ccgacccgcc cgaacccgat gaccgacggc
     1081 gccaaggtcc gcgccctgag ggttctgcag aacgcctgat ccctctccgc tcatccccgc
     1141 gaaagcgggg acccagttct ttgggcgata agcggtaggt gtagttcacg tcggcaatcc
     1201 aacgtccgag cttgtgaaag cactgggtcc ccgcttgcgc gggcatgagc ggaatattat
     1261 cagagctggc ccatgctgtt gtcgtgatgc acggcctgct gcttcagcca gtccacgaag
     1321 gcgacggcgc tcggcgaagc gggccggtcc ctcggcaaaa ccacgtgata ggcgaagccg
     1381 acagcctcgc tgccgtcggc gaacggcgcg accagtcgcc ccgcgctgag atcggcctga
     1441 gccagtgtcc gcttggcgag cgcgataccc cgtccggcca gagccccctc gatgaccatg
     1501 ctggcctgat tgaagcgagg cccgcgccgg gggtcgggat gcttaacccg ccgggccttc
     1561 agccacatgg cccagtccgg ccggctggga tcgccgtcct cggacatgtc gtgcagcagg
     1621 gtgtgatgga cgagatcggc gggcgtgcgg atcggatgct tgccgttcat cagcgacggc
     1681 gcggcgaccg gcaggacggt ctccagcatc aaccgctcga ccacatggcc cgggtaatcg
     1741 ccgggaccgt aacgcaccgc cagatcggcc ccgccctcgc ccagcgtcgc gggttccatg
     1801 tcggcggaga tccagacctc gatctcggga tggaccgcat ggaagtcgtc catgcgtggc
     1861 atcaaccatt tcgaggcgaa cgacggcgcg acgctgatcg agacctgttt cttgcgcggc
     1921 ggctcacgca ataacgacga ggcgtccatc aaccgctcga aaccctctcg cagcaaaggc
     1981 acggcgcctc gccccaaatc ggtcagttgc aggcccttgg cctcgcgctt gaacaggggt
     2041 ccgccgacga tgtcctcaag caagcgaatc tgctggctga cggccccggg tgtgaccgcc
     2101 agctcatccg cagcgcggga aaagttgagg tttcgcgctg cgctttcgaa ggcgcgcagg
     2161 gcattcaggg ggagcaacgg tctggccatg gccgttagaa atcctatcac cgcgacaaag
     2221 acaatctggt ttgtgcagcg cggcccataa gtcgagtgtc ctgacacgac ccacggacag
     2281 gcccagccta ccgatcgagg ccggtggagc ctgttatgag agtttttcta gcgtccattc
     2341 cgatgg
//
LOCUS       DASVTM010000244         4151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_23463, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000244 DASVTM010000000
VERSION     DASVTM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23463"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VF633_06745"
     CDS             <1..865
                     /locus_tag="VF633_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8470792.1"
                     /translation="VHPHYVRATETVVHAVGVETQALAAAIDAEAAVIDAIDKDTACV
                     VVQTPNVFGTATDVTKIAEAAHAAGALLIVVVTEAVSMGLLKSPGEMGADIVAAEGQS
                     IGNALNFGGPYVGLFACREKLIRQMPGRLTGETVDADGERGFVLTLSTREQHIRRDKA
                     TSNICTNSGLCTLAFTIHMSLLGETGLRKLALLNHEKALATRDALAAIPGVEILTTRF
                     FNEFAIRLPKNAAEVVSTLADHHVLAGVPYSRLAPEAGMDDVLLVAATETTMDVDIQI
                     LVKSLSKVLAA"
     gene            886..2448
                     /gene="gcvPB"
                     /locus_tag="VF633_06750"
     CDS             886..2448
                     /gene="gcvPB"
                     /locus_tag="VF633_06750"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX8470793.1"
                     /translation="MNTVGRPTAPNAVQSKPATLTGARGLLQDEALIFERDGWGKTGV
                     DLPEPTTDGSDLGDLIRRDPIGLPGLSEPETMRHYVRLSQKNHAIDLAIYPLGSCTMK
                     HNPRLNEKMARLPGFADIHPLQPISTVQGALELMDTLSHWLKTLTGMPAVALSPKAGA
                     HGELCGLMAIRAAHEASGEHAKRRKVLVPTSAHGTNPATAAFVGYTVVEVAQTEDGRV
                     DVADLASKLGDDVAAIMVTNPNTCGLFERDILEISRLTHEAGAYFYCDGANFNAIVGR
                     VRPGDLGVDAMHINLHKTFSTPHGGGGPGAGPVVLSAALAPFAPAPWVVHDDNGYSLI
                     EREEGDAAQAFGRLCAFQGQMGMYVRALSYMMSHGSDGLRQVAEDAVLNANYIKARLS
                     DLMSPAFPEGPCMHEALFDDEWLKGTDITTLDFAKAMIDEGFHPMTMYFPLVVHGAML
                     IEPTETESKQELDRFIHAMRLLAEAAKAGDVDRFKGAPFHAPLKRLDETRAARSPVLR
                     WTAPEGTNIAAE"
     gene            2495..3418
                     /locus_tag="VF633_06755"
     CDS             2495..3418
                     /locus_tag="VF633_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8470794.1"
                     /translation="MASFTLNDIPDLTGILAVVTGATGGLGLETALGLAGAGAEVALV
                     GRNPTKGRDAAALIRARHPAANVWFEQVDLASLASVKDFADEMLAEGRAIDLLINNAG
                     VMALPKRQTTADGFEMQFGVNYLSHFALTARLLPALRAGKARVVQLASIAHKGGRIHL
                     DDLNYETSYKAWPVYQQSKLAMLMFAIELQRRSDANGWGLTSVAAHPGFAHTNLVANG
                     PAAGGGPLYTAMTRVLEGFVGHSAAEGALSTLMAATLPDVTPGGYYGPQGWQDIKGPP
                     GPARASSQAQNAEVAARLWTASERLAGIKFG"
     gene            3632..4024
                     /locus_tag="VF633_06760"
     CDS             3632..4024
                     /locus_tag="VF633_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX8470795.1"
                     /translation="MTFATALPEYQPRPRDPLRALKRSALMVAGVLVVVLGILIAPLP
                     GPGGVPVIALGLVLILRSSYWAKRQFIRAQYARPKWVYPFRRLLRKNPEFAPVFWQQA
                     LRAEKLVMRRNEGPLKRIRKRLRRTARA"
BASE COUNT          723 a         1433 c         1296 g          699 t
ORIGIN      
        1 cgtgcatccg cactatgtcc gggccaccga gaccgtcgtc cacgccgtgg gcgtcgaaac
       61 ccaggcgttg gccgccgcga tcgacgccga agccgccgtc atcgacgcca tcgacaagga
      121 cacggcctgc gtcgtcgtcc agaccccgaa cgtgttcggc accgccaccg acgtgaccaa
      181 gatcgccgag gcggcccacg ccgccggcgc cttgctgatc gtcgtggtca ccgaggccgt
      241 gtcgatgggc ctgctcaagt cgccgggcga gatgggggcc gacatcgtcg ccgccgaagg
      301 tcagtcgatc ggcaacgccc tgaacttcgg cggaccctac gttggtctgt tcgcctgccg
      361 tgagaaactg atccgccaga tgcccggtcg tctgaccggc gagactgtgg acgccgacgg
      421 cgagcgcggg ttcgttctga cgctgtcgac gcgcgagcag cacattcgcc gcgacaaggc
      481 gacgtcgaac atctgcacca actccggcct ctgcaccctg gccttcacca tccacatgag
      541 cctgctcggc gaaaccggcc tgcgcaaact ggccctgctg aaccacgaga aggcgctggc
      601 cacccgcgat gcgctggcag ctattccggg cgtcgagatc ctgaccacgc gcttcttcaa
      661 cgagttcgcg atccgcctgc cgaagaacgc cgccgaggtg gtgtccaccc tggccgacca
      721 tcacgtgctg gcgggcgtgc cttacagccg tctcgcgccc gaggcgggaa tggatgacgt
      781 cctgctggtc gccgcgaccg agacgacgat ggatgtcgat atccagattc tcgtgaagtc
      841 cctttccaaa gtcctggccg cctgaggaac atgacgatga gcaccatgaa caccgtcggc
      901 cgcccgaccg ctccgaacgc cgtgcaatcc aagcccgcca ccctcaccgg cgcgcgtggt
      961 ctgttgcagg acgaagcctt gatcttcgaa cgcgacggct ggggcaagac cggcgtcgat
     1021 ctgcccgaac ctacgacaga cggctccgat ctgggcgacc tgatccgccg cgatccgatc
     1081 ggcctgccgg gcctctccga acccgagacg atgcgccact atgtgcgtct gtcccaaaag
     1141 aaccacgcta tcgacctggc catctatccg ctcggctcgt gcacgatgaa gcacaacccg
     1201 cgtctgaacg agaagatggc gcgcctgccg ggctttgccg acatccaccc gctgcagcct
     1261 atctcaacgg tgcagggcgc gctggagctg atggataccc tcagccattg gctcaagacc
     1321 ttgaccggta tgccggccgt ggccctgtcg cccaaggccg gcgctcacgg cgaactgtgc
     1381 ggtctgatgg ccatccgcgc cgcccacgag gcctcgggcg agcacgccaa gcgacgcaag
     1441 gtgctggtgc cgacctcggc ccacggcacc aacccggcca cggccgcctt cgtcggctac
     1501 acggtcgtcg aggtcgccca gaccgaagat ggccgcgtgg acgtcgccga tctggcctcc
     1561 aagctgggcg acgacgtcgc cgccatcatg gtgaccaacc ccaacacctg cggcctgttc
     1621 gagcgcgaca tcctcgagat cagccgcctg acgcatgagg cgggcgcgta tttctactgc
     1681 gacggcgcca acttcaacgc catcgtcggc cgggtccgcc cgggcgacct cggcgtcgac
     1741 gccatgcaca tcaacctgca caagaccttc tcgacgcccc acggcggtgg cggtccgggc
     1801 gcgggtccgg tcgtgctgtc ggcagctctc gctccattcg ctccagcgcc atgggtcgtg
     1861 cacgatgaca acggctattc gctgatcgaa cgcgaagaag gcgacgcggc tcaggccttc
     1921 ggtcgcctgt gcgccttcca gggccagatg ggcatgtacg tccgcgcgct cagctacatg
     1981 atgagccacg gctctgatgg cctgcgtcag gtcgccgagg acgctgtcct gaacgccaac
     2041 tacatcaagg cccgcctgtc ggacctgatg tcgccggcct tccccgaggg cccgtgcatg
     2101 cacgaggccc tgttcgacga cgaatggctc aagggcacgg acatcaccac gctcgacttc
     2161 gccaaggcga tgatcgacga gggcttccac ccgatgacca tgtatttccc gctggtcgtc
     2221 cacggcgcca tgctgatcga gccgaccgag acggaatcga aacaggaact ggatcgcttc
     2281 atccacgcca tgcgcctgct ggccgaggcc gcaaaagccg gcgacgtcga ccgcttcaag
     2341 ggcgcgccgt tccatgcgcc gctcaaacgc ctcgacgaga cccgcgccgc ccgctcaccg
     2401 gtgctgcgct ggaccgcgcc cgaaggcacg aacatcgcgg cggagtaggg cttaaagcct
     2461 tgcggtagtc agaggccatg ccgacatgat cggcatggcc tctttcacgc tcaacgacat
     2521 ccccgacctc acaggcatac tcgccgtcgt caccggcgcg acgggcggac ttgggctgga
     2581 gacggcgctg ggactggcgg gcgcgggcgc cgaggtcgct ctcgtcggtc gcaatcccac
     2641 caaggggcgt gacgccgcag ccttgatccg ggcacgccat ccggcggcga atgtctggtt
     2701 cgagcaggtg gatctggcca gcctggcctc ggtgaaggat ttcgccgacg agatgctggc
     2761 tgaaggccgg gctatcgacc ttctgatcaa caacgccggc gtcatggcct tgccgaagcg
     2821 ccagaccaca gcagacgggt tcgagatgca gttcggcgtc aactacctgt cccacttcgc
     2881 cctgacggca cggctgctgc ccgcgctgag ggcgggcaag gcgcgcgtgg ttcagttggc
     2941 cagcatcgcg cacaagggcg ggcgcatcca cctcgacgat ctgaattatg aaaccagcta
     3001 caaggcgtgg cccgtttatc agcaatcgaa actggcgatg ttgatgttcg ccattgaact
     3061 gcagcggcgc agcgatgcga acgggtgggg cctgaccagc gtcgcagcgc atcccggctt
     3121 tgcccatacg aatcttgtcg caaatggccc ggcggcgggc ggcggccctc tgtacacggc
     3181 gatgacgcgc gtgctcgagg gcttcgtggg tcattcggcg gctgaaggcg cactgtcgac
     3241 gcttatggcc gccaccctgc ctgacgtgac ccccggcggg tactatgggc ctcagggctg
     3301 gcaagacatc aaggggccgc ccgggcctgc cagagccagt tcgcaggctc agaatgccga
     3361 ggtcgcggcc aggctctgga ccgcatcgga acgcttggcc ggtatcaagt tcggctgatt
     3421 tccggcttaa ccgtgtctgt gcggacacgg ctcgcatgcg cctgcggaag cttacaaaag
     3481 cttcaccctt cagcctcggt tcagacacgg aagcagactg taaacgttgc tgtgacaagg
     3541 tcgtcacggt cgaagcttct tgcccagatc gtggacccgg atgaccccgg tgacgttgtc
     3601 gtatcagcct acctctccat ctggacgtcc cgtgaccttc gcaaccgccc tgccagagta
     3661 ccagccgcgt ccgcgcgatc cgttgcgcgc cctcaagcgc agcgcgctga tggtcgccgg
     3721 cgttctggtc gtcgtcctcg gcatcttgat cgcgccgctg ccgggtcccg gcggcgtgcc
     3781 ggtcatcgca ctgggattgg tgctgatcct gcgcagttcc tattgggcga aacgtcagtt
     3841 catccgcgct cagtacgcac ggccaaaatg ggtctatccc ttccgccgcc tgctgcgcaa
     3901 aaacccggag ttcgcgccgg tgttctggca gcaggcgctt cgggccgaga aactggtgat
     3961 gcgccgcaat gagggccctt tgaagcgcat ccgcaaacgc ctgcggcgga ccgcccgcgc
     4021 ctgatcgaac ccacccatca cggcgctatc tcgcgtgtgc gatgctgcac tggcgaagca
     4081 cggttttgtg aaatatgtct gaatggcgcc atgccaagac aggcgtagac tcctccttgc
     4141 gttatcggaa c
//
LOCUS       DASVTM010000245         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_237230,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000245 DASVTM010000000
VERSION     DASVTM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237230"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VF633_06765"
     CDS             complement(<1..723)
                     /locus_tag="VF633_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8470796.1"
                     /translation="MKTPFNNDRSTLMARLMCGTMFAVATLAVAMPTVAQTARPAPAP
                     QSVEEARVQADEAQEAAQVDDVVVTGTSIRGVAPVGSNVISVTQEDIQKTGAQTVQQL
                     LKSVPQVTGFNNAGQGGFGSADGSGTNAPTIHSLGASASNNTLILIDGNRVAPTGLNH
                     ALSDPSIVPPIALERVEVLPEGASSVYGSDAVAGVLNFITRRRYDGFEATAQVGFGDG
                     YRTYSGGLIAGTAWDTGSAFVAY"
     gene            1167..1409
                     /locus_tag="VF633_06770"
     CDS             1167..1409
                     /locus_tag="VF633_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470797.1"
                     /translation="MSVRPNRLPGPAHRALLVGACLFVLGIIAAPVATQVIDRRIDAD
                     SVWIDTGGVRGNRLASGVKANLEPAAAAAPSPGKRN"
     gene            1406..2260
                     /locus_tag="VF633_06775"
     CDS             1406..2260
                     /locus_tag="VF633_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX8470798.1"
                     /translation="MSATAEVGFADRPRRQLSLAHLTVLHASPPELIRLAACAGYDAV
                     GVRMTQVTPNEHVWPLTEDRAFFRETRAAIEETGVGILDVELVKLVPGFDVASFEPML
                     AAAAELGAQHVLTQAHDPDFSRVTASYAAFCDLAGRYGLTSDIEFLTWTDMRDLKSAT
                     ALLRAVDRPNSGLCIDSLHFDRSGCDVSDLAGLPPSWFHFAQIADAPAVAPSRADGLI
                     HTARAARLLPGDGSVDLKGLLRHLPASTPLAIEIPNAALSEVLSDEDRIRMAREALEA
                     LLVDIDGP"
     gene            2373..3848
                     /locus_tag="VF633_06780"
     CDS             2373..3848
                     /locus_tag="VF633_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX8470799.1"
                     /translation="MAPALRHYGHFIDGAETAPSSGAYFPSDNPYTGETWTMVAQGNA
                     ADVDAAVASSKAAFEGEWGQLRPSEKGRLMRRFADLLIEHAPRIAEIEQRDNGKLAAE
                     VVSQTRYVGDYFHYYAGLADKIQSAVIPTDKQGVFAYTKYEAKGVIGIITPWNSPLTL
                     TSWKLAPALAAGCTAVVKPSEFTSASMLEVAALFTEAGFPKGVVNVVTGFGAEVGEPL
                     VTHPDVAHIGFTGGEIGWRIYELAAKSRKTVTLELGGKSPNIVFDDADLDQAVKGVVS
                     GIFAATGQSCQAGSRLLVQDTIHDAFVARLIDFVSAAKLGDPSKMDTQIGPVATKPQF
                     DKIMGYIELGKEEGATLAMGGNCRPDLGPQFIEPTIFTNVRNDMRIAQEEIFGPVLSV
                     IPFKDEDDAIRIGNDVRFGLAAGVWTQNLKRAMKMSDQLKAGTVYVNNYRATSFTSPF
                     GGFKDSGIGRESGPDAIKEYLETKTVWMSSDLDVPNPFIRR"
     gene            3914..>4175
                     /locus_tag="VF633_06785"
     CDS             3914..>4175
                     /locus_tag="VF633_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8470800.1"
                     /translation="MLDLTNVLAGPFCTYQLVLLGAEVIKIETPGTGDLARVLGADQG
                     LVDDLMGASFLAQNSGKTSITINLKADEGKALFKRMVETADVV"
BASE COUNT          755 a         1316 c         1324 g          780 t
ORIGIN      
        1 gtaggcgacg aaggccgagc cggtgtccca agcggtcccg gcgatcagcc cgccgctata
       61 ggtcctgtag ccgtcgccga acccaacctg ggcggtggct tcgaacccgt cgtagcggcg
      121 gcgggtgatg aagttcagca cacccgcgac ggcgtccgag ccatagaccg aggatgcgcc
      181 ttcgggcagc acctcgacgc gctccagcgc gattggcggc acgatcgaag ggtctgacag
      241 cgcgtggttg aggccggtcg gagcgacccg gttgccgtcg atcaggatca gagtgttgtt
      301 tgaggcggac gccccaaggc tgtggatggt cggcgcgttg gtcccggatc catcggcgct
      361 gccgaacccg ccctgaccgg cattgttgaa gcccgtgacc tgaggaacgc tcttcagtag
      421 ttgctggact gtctgggcgc ccgtcttctg gatgtcttcc tgcgtaacgc tgatcacgtt
      481 ggatccgacc ggtgcgacgc cccggatgct ggtgccagta acgacgacat catcgacctg
      541 agcggcttcc tgagcctcat cagcctgaac ccgggcttct tcgaccgact gcggagccgg
      601 cgctggtcgg gccgtctggg cgacggtggg catggcgaca gcgagcgtag cgaccgcgaa
      661 catcgtaccg cacatcaggc gcgccatcag cgtcgaccga tcgttgttga atggtgtctt
      721 catgacgtgt cttcccctgg ggcttttaat acgacgaccg gatttcctcc ggcctgtcgg
      781 gctgttcgtt ctggttcgta cattctagga tcgcacggac acgcgccgaa aatcggacgc
      841 gcacacatcg gatcggtcga aaaagctttg tctcaactcc cggccggaca tgaggtgcac
      901 tcgcctgacg gcgcgcgacc ctgcccgagc tcatttgtgc catgatcctt acggacgaaa
      961 tggtacatgt caacgcggcg atgccccgtg gcgtcccttt gactctttcg atgctttcag
     1021 aacccagtcc cggcgggcat ttgccattcg ataaaaatgt aatatccagt acgttctacg
     1081 ttcgtgcgga tcagtgagaa gagcgaaatg ctcctgctgc ggaggaacaa gccgtcggcg
     1141 atcgtgccga ccaaggagtc gcgaaaatga gcgtccgccc taaccgcctg cccggtccgg
     1201 cgcatcgggc gctgcttgtc ggagcctgcc ttttcgtcct gggcatcatt gcggctcctg
     1261 tggccaccca ggtcattgat cgccgcatcg acgcagactc cgtctggatc gacaccgggg
     1321 gagtaagggg caatcgcctc gcctcggggg tgaaagccaa tctcgagccg gccgccgccg
     1381 ctgccccgag cccggggaaa cgcaattgag cgcaaccgcc gaagtcgggt ttgcagacag
     1441 gccgcgacgc cagctttcgc tggcgcacct tacggtcctg catgcctcgc ccccggaact
     1501 gatccggctc gcagcgtgtg cgggttatga cgccgttggt gtccgcatga cccaggtcac
     1561 cccaaacgaa cacgtgtggc cgttgaccga ggacagagcg ttctttcgcg agacacgcgc
     1621 cgctatcgaa gagaccggcg tcggcattct ggacgtcgaa ctggtcaaac tggttccggg
     1681 tttcgacgtg gcgtccttcg agccgatgct ggcggcggcg gcggagcttg gcgcgcagca
     1741 tgtcctgacc caggcccatg atccggactt cagccgcgtg acggccagct acgcggcctt
     1801 ctgtgatctg gccggtcgct atgggctgac cagcgacatc gagttcctga cctggacaga
     1861 catgcgcgac ctgaagagcg caacggcgtt gttgcgggcc gtcgaccggc ccaacagcgg
     1921 cctgtgtatc gacagtctgc atttcgatcg gtccggctgc gacgtatccg acctcgcggg
     1981 tctgccgccg tcatggttcc atttcgccca gatcgcggac gcccccgccg tggctccttc
     2041 acgggccgat ggattgatcc acaccgctcg agcagcccgc ctgctgcccg gcgatggaag
     2101 tgtcgatcta aagggcctgc taaggcacct tcccgccagc acacctctgg ccatcgaaat
     2161 tcccaatgcg gcgctgagtg aagtcctgtc tgacgaggac cggattcgca tggcgcgaga
     2221 agcgcttgag gcccttttag tcgacatcga cgggccttga tgagcgcgat cccaggataa
     2281 atctaaacgg cccttggcaa taaatgtaat agttagtaca tgactgaaac gggcagcgcg
     2341 tgacgcgctc ccggcgtcct caagagagac caatggctcc tgcccttcgc cactacggac
     2401 actttatcga cggggctgaa acggcgccgt cgtccggcgc ctatttcccc agcgacaacc
     2461 cctacacggg cgagacctgg accatggtcg cgcagggcaa tgccgccgat gtcgacgctg
     2521 cagtggcctc gtccaaggcg gcgttcgaag gcgaatgggg ccaactgcgg cccagcgaaa
     2581 agggacggct gatgcgtcgt ttcgccgatc tgctgatcga acatgcgccg cggatcgccg
     2641 agatcgagca gcgggacaat ggcaaactgg ccgccgaagt cgtgtcccag acccgctatg
     2701 tcggagatta tttccactat tacgccgggt tggcggacaa gattcagagc gcggtcatcc
     2761 ccaccgacaa gcagggggtg ttcgcctata cgaagtatga ggcgaagggg gtcatcggca
     2821 tcatcacgcc gtggaactcg ccgctgacgc tgacgagctg gaagttggcc cctgcccttg
     2881 cggccggctg caccgccgtc gtcaagccgt cggagttcac ctcggcgtcg atgctggaag
     2941 tcgcggcgct gttcacagag gcgggctttc ccaagggcgt cgtcaacgtc gtgaccggct
     3001 tcggggcgga ggtcggcgag cccctcgtga cccatccgga cgtcgcccat atcggcttca
     3061 cgggcggcga gatcggctgg cgcatctatg agctggcggc gaagagccgc aagacggtga
     3121 ctctggagct gggcggcaag tcgcccaaca tcgtgttcga cgatgccgat ctcgatcagg
     3181 ccgtgaaggg cgtcgtctcc ggcatttttg cggccactgg ccagagctgt caggcgggtt
     3241 cgcggctgct ggtgcaggac acgatccatg acgccttcgt ggcccggctg atcgacttcg
     3301 tctctgccgc caagctcggt gatccgtcga agatggacac ccagatcggt ccggttgcca
     3361 ccaagccgca gttcgacaaa atcatgggct atatcgagct cggcaaggaa gagggcgcga
     3421 ccctggccat gggcggcaac tgccgtcccg acctcggccc ccagttcatc gaaccgacca
     3481 tcttcaccaa cgtgcgcaac gacatgcgta ttgcgcagga agagatcttc ggacctgtcc
     3541 tgtcggtgat cccgttcaag gacgaggacg acgccattcg catcggcaac gacgtccgct
     3601 tcggccttgc cgccggcgtc tggacccaga atctgaagcg cgcgatgaag atgtcggacc
     3661 agctcaaggc gggcaccgtc tatgtgaaca actaccgcgc cacgtctttc acctcgccct
     3721 tcggcggctt caaggattcg ggcatcggtc gcgagtctgg ccccgacgcg atcaaggaat
     3781 acctcgaaac caagaccgtc tggatgtcga gcgacctcga cgtgcccaat cccttcatcc
     3841 gccgatgagc gctgtcgggg aagtcgtatc gacatcggcc ggaggactgc caccagtcct
     3901 cgctggcgtt cgcgtgcttg acctgaccaa cgttctggca gggccgttct gtacgtatca
     3961 gttggtcttg ctcggcgccg aggtcatcaa gatcgagacc ccggggacag gcgatctggc
     4021 gcgggtgctg ggcgccgatc aggggcttgt cgacgacctg atgggcgcgt cattcctggc
     4081 tcagaactcg ggcaagacct cgatcacgat caatctcaag gccgatgagg gcaaggcgct
     4141 gttcaagcgg atggtcgaga ctgccgacgt ggtgg
//
LOCUS       DASVTM010000246         5478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_341503,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000246 DASVTM010000000
VERSION     DASVTM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341503"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /gene="fusA"
                     /locus_tag="VF633_06790"
     CDS             <1..1445
                     /gene="fusA"
                     /locus_tag="VF633_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8470801.1"
                     /translation="KLVEACVEMDDDAMTAYLDGVEPSIEVLKKCIRKAVLTGAFYPI
                     LCGSAFKNKGVQTLLDAVVDYLPSPLDIPPTPGIDFKTEEPVVRRASDDEPLSILAFK
                     IMDDPFVGSLTFCRLYSGKMETGQNLLNSSRDKRERVGRMLQMHSNNREDVKEAYAGD
                     IVALAGLKDTRTGDTLCDPLKSPVILEKMEFPAPVIEISVEPKTKADQEKLGVALAKL
                     ASEDPSFTVSTDHESGQTILKGMGELHLDIKIDILKRTYKVEATIGAPQVAYRESLGR
                     KVDIDYTHKKQTGGTGQFARVMITFEPGEPGSGFVFENSIVGGAIPKEYIPGVEKGLN
                     SIKDGGLLAGFPLIDFKASLTDGKYHDVDSSVLAFEIAARAAFRELKEKGAPKLLEPI
                     MAVEVVTPEDYLGSVIGDLNGRRGMIQGQDMRGNATVVNAFVPLANMFGYVNTLRGMS
                     QGRAQFTMQYDHYEPVPQHVADEVIKKYSA"
     gene            1536..2726
                     /gene="tuf"
                     /locus_tag="VF633_06795"
     CDS             1536..2726
                     /gene="tuf"
                     /locus_tag="VF633_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX8470802.1"
                     /translation="MAKEKFERNKPHCNIGTIGHVDHGKTTLTAAITMVLAKASGGKA
                     MNYADIDAAPEEKARGITINTAHVEYETAARHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDLVDDDELLELVEMEVRELLSS
                     YQFPGDDIPITMGSAKAATDGVNPEIGEQRVLALMETVDAYIPQPARPLDLPFLMPVE
                     DVFSISGRGTVVTGRIEKGIVKVGEEVEIVGIRPVQKTTCTGVEMFRKLLDQGQAGDN
                     VGVLLRGTKREDVERGQVLCKPGSITPHTKFVAEAYILTKEEGGRHTPFFTNYRPQFY
                     FRTTDVTGIVHLKEGVEMIMPGDNAELNVELITPIAMDQGLRFAIREGGRTVGAGVVA
                     KIIA"
     gene            2839..4452
                     /locus_tag="VF633_06800"
     CDS             2839..4452
                     /locus_tag="VF633_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8470803.1"
                     /translation="MIRTACSVLLILFGTLLSGCALHPGTVERVRMASDLSRPIVATP
                     SGALRGIDGKGVRAFLGVPYAASPVGDLRWRAPRPVAAWDGVRDASRIGSDCTQALGR
                     RAILGGGGGIVFGGEDCLYLNIYAPAGEPAKPRPVMVYLHGGAFTIGAGANYDPSLLA
                     VEQDRVVVTLNFRLGALGWLAHPGFDAEADGVGGNWGLMDQQAALRWVRDNIAAFGGD
                     PADVTLFGESSGAWSACYLMAAPSSQGLWNRVILQSGPCLEPSSLNSAAVAAEAGPSF
                     AHLLGCAEPDAVACLRATSNKKIAKTRSTRGGLNGPGSWGPVYGDALVPISPATAFAS
                     GDFIRTPVIVGTNADEGRLFAVEVKDMARYDDETRWMYGAEGGRVLSRYPVGAEGPGL
                     TMARSFTDQRFACPSQALRRVLSKYVPTYGYEFVDPHAPIVVPRFLLPYDMGAYHAAE
                     ATYVFGRPWAFANPSKFTPEQAALARRMRDAWASFGRAEFDAEWPAVAANGAPLKVFD
                     PQGDRMDETFFDRHHCDLWDGTPFGGVTH"
     gene            complement(4456..>5478)
                     /locus_tag="VF633_06805"
     CDS             complement(4456..>5478)
                     /locus_tag="VF633_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8470804.1"
                     /translation="PSAPAPAAQQQSAQMQTTPGLARADIEGATPAATPAAGPAAPAA
                     GTVPSGTPAAGTPAAAMKPNAGMIRLQILLDRSAFSPGQIDGLHGENTRQAIEAYREA
                     NNLGTGDVPDAALLQSLTATDAGPVTQDYVLTTADVSGPFSPPPTEDLKAQAAAGTNY
                     STALERIAERFHVSEALLQTLNPGVNFQSAGARILVPALNMTPLASVARIEVDKSAKA
                     IRAYDASDKMIAFYPATIGSGDNPSPSGEVKINGVARAPDYTYDPAKLSYGPGGDKVI
                     VPAGANNPVGAVWIDLDRPSYGIHGTPDPTKIGKSASHGCVRLTNWDAQQLAAAVKPG
                     VMVRFV"
BASE COUNT          964 a         1756 c         1800 g          958 t
ORIGIN      
        1 tcaagctcgt cgaggcctgc gtcgagatgg atgacgatgc catgacggcc tacctcgacg
       61 gcgtcgagcc ttcgatcgaa gtgctgaaga agtgcatccg caaggccgtg ctgaccggcg
      121 ccttctatcc gatcctgtgc ggctcggcgt tcaagaacaa gggcgtgcag acgctgctcg
      181 acgccgtcgt cgactacctg ccttcgccgc tcgacattcc gcctacgccc ggcatcgact
      241 tcaagaccga agaacccgtc gtgcgtcgcg cctcggacga cgagcccctg tcgatcctcg
      301 cattcaagat catggacgac ccgttcgtcg gctcgctgac cttctgccgc ctatactcgg
      361 gcaagatgga aaccggtcag aacctgctga actcgtcgcg cgacaagcgt gagcgcgttg
      421 gtcggatgct gcagatgcac tcgaacaacc gtgaagacgt caaggaagcc tatgccggcg
      481 acatcgtcgc tctggctggc ctcaaggaca cccgcacggg tgacaccctg tgcgatccgc
      541 tgaagtcgcc cgtcatcctc gagaagatgg aattcccggc cccggtcatc gagatctcgg
      601 tcgaacccaa gaccaaggcc gaccaggaaa agctgggcgt cgccctggcc aagctggctt
      661 cggaagatcc gtccttcacc gtctcgaccg accacgagtc gggccagacc atcctgaagg
      721 gcatgggcga gcttcacctc gacatcaaga tcgacatcct gaagcgcacc tacaaggtcg
      781 aagccaccat cggcgcaccg caggtcgcct atcgtgaatc gctcggccgc aaggtcgaca
      841 tcgactacac ccacaagaag cagaccggcg gtacgggcca gttcgcccgc gtcatgatca
      901 ccttcgaacc cggcgaaccc ggctcgggct ttgtgttcga gaactcgatc gtcggcggcg
      961 ccattcccaa ggaatacatc ccgggcgtgg aaaagggtct gaactccatc aaggacggcg
     1021 gcctgctggc cggcttcccg ctgatcgact tcaaggccag cctgacggac ggcaaatacc
     1081 acgacgtcga ctccagcgtg ctggcgttcg aaatcgcggc ccgcgccgcc ttccgcgaac
     1141 tgaaggaaaa gggcgctccc aagctgctcg agccgatcat ggccgtcgag gtcgtgaccc
     1201 ccgaggacta cctcggttcg gtcatcggcg acctgaacgg tcgtcgcggc atgatccagg
     1261 gtcaggacat gcgcggcaac gctacggtcg ttaacgcgtt cgtcccgttg gccaacatgt
     1321 tcggctatgt gaacacgctt cgcggcatgt cacagggccg cgcccagttc accatgcagt
     1381 acgaccacta cgaaccggtg ccgcaacacg tcgccgacga agtgatcaag aagtattccg
     1441 cctaaaccca cctaatcccg gacgatcccg agggtcgtcc ggacccctga aaatccagaa
     1501 ataatgcccc cttttcaggg gacggagaga agaaaatggc caaggaaaag ttcgaacgta
     1561 acaagccgca ttgcaacatc ggcacgattg gtcacgtcga ccacggcaag acgacgctga
     1621 cggcggcgat cacgatggtt ctggccaagg ccagcggcgg caaggcgatg aactacgccg
     1681 acatcgacgc agcgcccgaa gaaaaggctc gcggcatcac gatcaacacg gcgcacgtgg
     1741 aatatgagac ggctgcccgt cactatgcgc acgtcgactg cccaggccac gccgactatg
     1801 tgaagaacat gatcaccggc gcggctcaaa tggacggcgc gatcctggtg gtttcggccg
     1861 ccgacggccc gatgccccag acccgcgagc acatcctgct ggcccgtcag gtcggcgtgc
     1921 cggctctggt cgtgttcatg aacaaggtcg acctggtcga cgacgacgag ctgctcgagc
     1981 tggtcgagat ggaagtgcgc gagctgcttt cgtcctacca gttcccgggt gacgacattc
     2041 cgatcaccat gggttcggcc aaggccgcga ccgacggcgt gaacccggaa atcggcgagc
     2101 aacgcgtgct ggccctgatg gaaaccgtcg acgcctacat cccgcaaccg gcccgtcctc
     2161 tggacctgcc gttcctgatg ccggtggaag acgtgttctc gatctcgggt cgcggtacgg
     2221 tcgtcaccgg ccggatcgaa aagggtatcg tcaaggtcgg tgaagaagtc gaaatcgtcg
     2281 gcatccgtcc ggtccagaag acgacctgca cgggcgtcga aatgttccgc aagctgctcg
     2341 accagggtca agcgggcgac aacgtcggcg tgctgctgcg tggcaccaag cgtgaagacg
     2401 tcgagcgcgg ccaggttctc tgcaagcccg gctcgatcac cccgcacacc aagttcgtcg
     2461 ccgaagccta catcctgacc aaggaagaag gcggccgtca cacgccgttc ttcacgaact
     2521 atcgccctca gttctacttc cgcaccacgg acgtgaccgg catcgttcac ctgaaggaag
     2581 gcgtcgaaat gatcatgccc ggcgacaacg ccgagctgaa cgtcgaactg atcactccga
     2641 tcgctatgga ccagggcctg cgcttcgcca tccgtgaagg cggccgcacc gtcggcgccg
     2701 gcgtggtcgc caagatcatc gcctaaaagc gaagatagac agtaagcgta cagagcggcc
     2761 ccggagagca atctccgggg ccgttttgct ttgctgggcg acagcaacct tgaccatttt
     2821 ccacacgttg ggagccggat gatccgcacc gcctgttccg ttctcctgat cctgttcggc
     2881 accctgcttt cggggtgcgc tctgcatccc ggtacagtgg agcgggtgag gatggcgtcc
     2941 gatctcagta gacccatagt cgccacccca tccggcgcct tgcgcgggat cgacggaaag
     3001 ggcgtgcgcg cgtttctcgg cgttccctat gccgcgtcac cggtgggcga tctgcgttgg
     3061 cgggcgccga gacctgtcgc agcgtgggac ggtgtccgcg acgcttcccg catcggcagc
     3121 gactgcaccc aggcgcttgg gcgtcgcgcc atcctcggcg gcggtggcgg gatcgtcttt
     3181 ggaggcgagg actgcctcta cctcaacatc tatgcgccgg ccggagagcc cgcgaagccg
     3241 cgcccggtca tggtctatct ccacggcggc gccttcacca tcggcgcggg cgccaactac
     3301 gaccccagtc tgctggcggt cgagcaggac cgggttgtgg tcacgctgaa cttccgtctc
     3361 ggcgctctgg gctggctggc tcatccgggc ttcgatgccg aggctgacgg cgttggcggc
     3421 aactggggtc tgatggacca gcaggcggcg ctgcgctggg ttcgcgacaa tatcgcggct
     3481 ttcggtggcg atccggcgga cgtcaccctg ttcggagaaa gctccggggc ctggagcgcc
     3541 tgctacctga tggcggcgcc gtcgtcgcaa ggcctttgga accgggtcat cctccagagc
     3601 gggccgtgtc tggagccgtc gtctctgaac agcgctgcgg tggctgccga ggctggaccc
     3661 agcttcgctc acctgctggg ctgtgccgaa ccggacgccg tggcctgtct gcgcgccacc
     3721 tcgaacaaga agatcgccaa gacccgctca acacgcggcg ggctgaatgg tccgggttcc
     3781 tggggccccg tatatggcga cgctctggtt ccgatcagcc cggcgacggc cttcgcttcc
     3841 ggggacttca tccgcacccc cgtcatagtc ggcaccaacg cggatgaggg gcggctgttc
     3901 gcggtcgagg tcaaggacat ggctcgctac gacgacgaga cgcgctggat gtacggcgcc
     3961 gaaggtgggc gcgtgctgtc gcgctatccg gtgggcgccg aaggtcccgg cctgacgatg
     4021 gcgcgcagct tcaccgacca gaggttcgcc tgtccgtcgc aggctctgcg gcgggtgctg
     4081 tcgaaatatg tcccgacgta cggctacgag ttcgtcgatc cgcatgcgcc gatcgtcgtg
     4141 ccccgctttc tgctgccgta cgacatgggg gcctatcatg ctgcggaggc gacctatgtg
     4201 ttcgggcggc cgtgggcctt tgcaaacccg agcaagttca ctccggagca ggccgcgttg
     4261 gcgcgtcgga tgcgcgacgc gtgggcgagc tttgggagag cagagttcga cgcggaatgg
     4321 cccgctgtgg ccgccaacgg cgcgccgctg aaggtgttcg accctcaggg agatcgaatg
     4381 gacgagacct tcttcgatcg tcaccactgc gacctgtggg acggcacccc gttcggcggc
     4441 gtcacccact agccgctaga cgaagcgcac catcacgccc ggcttgacgg ctgcggcgag
     4501 ctgttgcgca tcccagttgg tcaggcggac acagccgtgg ctggcgctct tgccgatctt
     4561 ggtcggatcg ggcgtcccgt gaatgccgta gctggggcgg tcgaggtcga tccacaccgc
     4621 accgaccggg ttattcgcac cggccggaac aatgacctta tcgccgccgg ggccatagct
     4681 gagcttggcg gggtcatagg tgtagtcggg cgcgcgggcg acaccgttga tcttgacctc
     4741 gcctgacggg ctggggttgt cgccgctgcc aatcgtggcg ggatagaagg cgatcatctt
     4801 gtccgaggcg tcgtaggcgc ggatggcctt tgcgctcttg tccacctcga tgcgcgcgac
     4861 cgaggccagc ggcgtcatgt tgagggccgg gacgaggatg cgggcgccgg ccgactggaa
     4921 attgacgccg gggttcaggg tctgaagcag ggcttcggag acgtggaagc gctcggcgat
     4981 ccgttccaac gcggtggagt agttggtccc ggcggcggct tgcgccttga ggtcctcggt
     5041 cggtggcggg ctgaaggggc cggagacgtc tgcagtcgtc agcacatagt cttgcgtcac
     5101 cgggccggcg tcggtggcgg tcaacgactg cagcagggcg gcgtcgggga catcgcccgt
     5161 gccgaggttg ttggcttcgc gataggcctc gatggcctga cgggtgtttt cgccgtgaag
     5221 cccatcgatc tggcccggtg agaatgcgct gcggtcgagc aggatctgga gccggatcat
     5281 gcctgcattc ggcttcatcg cagcggcagg cgtccccgct gcgggcgttc ctgaaggcac
     5341 ggttccggcg gcaggtgctg caggtccggc cgccggcgtc gcggccgggg tcgcgccttc
     5401 gatgtcggct cgggcgaggc cgggcgtcgt ttgcatctga gcgctttgtt gctgagcagc
     5461 cggggccgga gccgacgg
//
LOCUS       DASVTM010000247         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_504520,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000247 DASVTM010000000
VERSION     DASVTM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504520"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VF633_06810"
     CDS             <1..333
                     /locus_tag="VF633_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX8470805.1"
                     /translation="ERGWRLVADDYCDVWASGGVVWATAPVRIAGLLEARGLGIIRSA
                     PLPIVRIVLVVACGHASVERLPEPAIEIIAGTILPHITLDTRAASAPETLHAALKVTL
                     GGLDVAPL"
     gene            443..847
                     /locus_tag="VF633_06815"
     CDS             443..847
                     /locus_tag="VF633_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX8470806.1"
                     /translation="MIGLVIVTHGGLASEFLAAMEHVVGPQRGVAAICIGPEDDMERR
                     RRDIVDAADAVNDGSGVILLTDMFGGTPSNLAISVMEQTHAEVIAGLNLPMLIKLASV
                     RSRETLEACVAHAQDAGRKYISVASWVLAGEK"
     gene            859..1134
                     /locus_tag="VF633_06820"
     CDS             859..1134
                     /locus_tag="VF633_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX8470807.1"
                     /translation="MPNASTTANICNTRGLHARASAKFVKLASSFEAEIHVTRDGTTV
                     DARSIMGLLMLGAGNGSSVDITAEGSDAAEAIEALNELVATRFGEDE"
     gene            1194..1985
                     /locus_tag="VF633_06825"
     CDS             1194..1985
                     /locus_tag="VF633_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX8470808.1"
                     /translation="MTDQILDETPDKDAVPVQASHGDRPLPRGRTATYRHPAWVRISH
                     WINVVAVVVLLMSGANILLAHPHLYWGLRSTFADPILNIPTIPDWLLIPQGRNLADAR
                     RWHFFFAWLFVFNGLAYLIFGFLTRRFQRRIWPTVSDLKGFWPSVKEHVRFHFAKDDE
                     ARRYNVLQKLTYAAVVLVVLPMMMITGLSMSPGFNSIGEVLLNLMGGRQSARTLHFIS
                     AGLIIAFIVVHVGLVIWTGLWNNMRAMITGWYVIEPEDGEPGNKP"
     gene            1982..2752
                     /locus_tag="VF633_06830"
     CDS             1982..2752
                     /locus_tag="VF633_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8470809.1"
                     /translation="MSEHGFSRRGWLTRALATVGVMALSACDRGAELGTRLLVSAESL
                     TKAAQRLILPRDALAPEYDVSHISRDFKPNGSIDPAAADYLALKAGSFAHYRLEIGGL
                     VERPLSLSLGDLRARPSRTQITKHDCVEGWTSIGQWSGAKLSALLDEAGLKPEAKYIV
                     FHCFDALTQTEDGPRYYESIDLVDAYHPQTILAYDMNGEVLRVPHGAPLRLRVERQLG
                     YKHAKYIRRIEAVESFDAIEGGKGGYWEDRGYEWYAGI"
     gene            complement(2749..>2988)
                     /locus_tag="VF633_06835"
     CDS             complement(2749..>2988)
                     /locus_tag="VF633_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8470810.1"
                     /translation="VGSAGWGVDPLSPIFVEGETGDRSPAPGPAGDYPAYYAAVAEAL
                     RGDGPNPVPPSEALLVMEVLEAGMQSSRERREVLL"
BASE COUNT          531 a          939 c          960 g          558 t
ORIGIN      
        1 gaacgaggct ggcggctggt cgcagacgac tattgcgacg tttgggcgtc gggaggagtc
       61 gtttgggcca cagctccagt acggatcgcc gggctgctgg aagcgcgtgg tctaggcatt
      121 atccgatccg ccccccttcc aatagttcgc atcgttctgg tggtggcgtg cggtcatgca
      181 tcggttgagc ggctgcccga gccggcgatc gaaatcatcg ccgggaccat ccttccgcat
      241 atcaccctgg atacccgcgc agcctcggcc ccggagacac tgcacgccgc ccttaaggtc
      301 acacttggcg ggcttgatgt cgcaccgctt taaagtcctg tgcgtttcct ctatctaggc
      361 cggcttgcgg tccggtcggg gctcccttcc cgacggacag acgcattttt cggccggggg
      421 gcgtagtgtt ggtgaagcct tcatgatcgg gctggtgatc gtcacccacg gaggtctcgc
      481 ctccgaattc ctggcggcga tggagcatgt cgtgggccct cagcgaggcg tcgcggccat
      541 ctgcatcggc cccgaagacg atatggagcg ccgccgacgc gacatcgtcg acgccgccga
      601 cgccgtcaac gacggctcag gcgtcatcct cctgaccgac atgttcggcg gcaccccgtc
      661 caatctcgcc atctcggtga tggaacagac ccacgccgag gtcatcgccg gactgaacct
      721 gcccatgctg atcaagctcg ccagcgtgcg gagccgtgag acgctggaag cctgcgtcgc
      781 tcacgcccag gacgccgggc gcaaatacat ctccgtcgcc tcctgggttt tggcgggcga
      841 gaagtaggaa ccggcgccat gcccaacgca tcgaccaccg ccaacatctg caacacgcga
      901 gggcttcatg cccgggcctc ggccaaattc gtcaagctgg cctccagctt cgaagccgag
      961 atccatgtca cgcgcgacgg caccaccgtg gacgcccgct cgatcatggg actgctgatg
     1021 ctgggcgccg gcaatggctc cagcgtcgac atcacagctg aaggttcgga cgcggccgaa
     1081 gctatcgaag cgctgaacga actcgtcgcc acccggtttg gcgaagacga ataggggtaa
     1141 cctcgcggcg ttgcccggcg taacctcctg aaccagtcgc agtgagggcc ttgatgacgg
     1201 atcagatcct cgacgaaact ccggacaagg atgccgttcc cgttcaggca tcgcatggtg
     1261 accgccccct tccccgtggt cgaaccgcga cctatcggca tcccgcctgg gtccgcatca
     1321 gccactggat caacgtcgtc gccgtggtcg tgctgcttat gagcggcgca aacatcctgt
     1381 tggcccatcc gcatctatat tgggggctac ggtcgacttt cgccgacccg atcctgaaca
     1441 tccccaccat tcccgactgg ctgttgatcc cgcagggacg caatctggcc gacgcacggc
     1501 gctggcattt cttcttcgcc tggctgttcg tgttcaacgg cctcgcttat ctgatattcg
     1561 gcttcctgac ccgccggttt caacggcgga tctggccgac ggtgtccgat ctcaagggct
     1621 tctggccgtc ggtgaaggag cacgtccgtt tccatttcgc caaggatgat gaggcgcggc
     1681 gctacaatgt gctgcagaag ctgacctatg cggcggtggt gctggtggtg ctgccgatga
     1741 tgatgatcac cggcctgtcg atgtcgccgg ggttcaactc catcggcgag gtgctgctga
     1801 acctgatggg cgggaggcag tcagcgcgga cgctgcactt catctcagcg ggactgatca
     1861 tcgcgttcat cgtcgtccat gttggattgg tgatctggac cgggctctgg aacaacatgc
     1921 gggcgatgat caccggctgg tatgtgattg agccagaaga tggtgagccg gggaacaagc
     1981 catgagcgag catggcttca gccgacgcgg ctggctgacc cgggctctgg ccacggtggg
     2041 ggttatggcg ctgtcggcct gtgatcgtgg ggcagagtta gggacgcgat tgctggtcag
     2101 cgccgaaagc ctgaccaagg ctgcgcaacg gttgatcctg cctcgtgacg ctctggctcc
     2161 cgagtatgac gtcagccaca tatcccgcga tttcaaaccc aacggctcca tcgacccggc
     2221 ggcggcggac tatctggcgc tcaaggcagg aagtttcgcg cactatcggt tggagattgg
     2281 cgggctggtc gagcggccgc tgagcctgtc gctgggggac ctgcgggcca ggccgtcgcg
     2341 aacccagatc accaagcatg actgcgtcga gggctggact tcgattggcc agtggtcagg
     2401 ggcgaagctg tccgccctgc tggacgaggc ggggttgaag cccgaagcga aatacatcgt
     2461 cttccactgc ttcgacgccc tgacccagac cgaagacggg ccgcgctatt acgagagcat
     2521 cgatctggtc gacgcctatc acccccagac catcctcgcg tacgacatga acggcgaagt
     2581 cctcagagtc ccgcacgggg cgccgctcag gctgagggtc gagcgacagc ttggctacaa
     2641 gcacgccaaa tacatccggc ggatcgaggc ggtcgaaagc ttcgacgcga tcgagggcgg
     2701 taagggcggc tactgggaag accggggcta cgaatggtat gcagggatct agagcaggac
     2761 ttcccggcgt tcacggctgc tctgcatccc cgcttccagc acttccatga cgagtagggc
     2821 ttcggacggc gggaccgggt tggggccgtc accgcgtaac gcctcagcca cggcggcata
     2881 gtaggcggga tagtcgccag ccggtccggg agccggtgag cgatcgccag tttcgccttc
     2941 tacgaagatc ggcgacaggg gatcaacgcc ccagcctgca gagccgac
//
LOCUS       DASVTM010000248         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_341637,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000248 DASVTM010000000
VERSION     DASVTM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341637"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VF633_06840"
     CDS             complement(<1..573)
                     /locus_tag="VF633_06840"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX8470811.1"
                     /translation="MAENPEPLPIVRTVSELRAIVREWRRQGLTVGLVPTMGALHDGH
                     LTLVREAGCRADRVVASVFVNPTQFAAHEDLGTYPRQEARDAELLSGAGCALMFAPTV
                     AEMYPDGFVTSVAVGDRSKGGPSQGLEGDFRPQMFGGVALVVSKLLNQVQADVAVFGE
                     KDWQQLMVVRRMVADLDIPTEIVGAPTLRDD"
     gene            664..1383
                     /locus_tag="VF633_06845"
     CDS             664..1383
                     /locus_tag="VF633_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470812.1"
                     /translation="MLRPALVCLALSGIGLTTASCGMVESNPHRFETLADKVADIQLD
                     ASGEVPPGNITVPSTLTAAEQHLRPATSAASEPPSRGLRVEVMDPHDLWDARDGLRRT
                     IREESTAVAEAAAPMMARAVVQQVSHRLSGSPASAGRTAQRAIVREVEGRSPAQTVIQ
                     PRQLIQLGAYSSEASARSAWTRVSNGAARQALSGLSPMFEQVTVGGRPLTRLKVAAPA
                     TAATAICRAVAISDPWCARRT"
     gene            1542..2387
                     /locus_tag="VF633_06850"
     CDS             1542..2387
                     /locus_tag="VF633_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEX8470813.1"
                     /translation="MAQPQVVVIGNEKGGAGKSTLAIHIVVGLLHAGHRVAIIDLDSR
                     QRSMSHFFSNRAAWTAANGHTLPMPIEPDMGDGKALAKASEEDQLAIFEAAYLQAQDS
                     DIIVIDTPGGDTALSRAAHGRADQIVTPMNDSFVDFDMLGQVDPVTLDLLKPSTYSES
                     VWEARKHRAITEGRHASIDWLVVTNRLAVAEARNRRRLEERMMKLAKRVGFRVGPGLR
                     DRVIYRELFPFGLTVADLSNDIRPVAVSLAHVAARQEMRSLMLAMGLDQASLDAKEPA
                     LDAAA"
     gene            2397..>2661
                     /locus_tag="VF633_06855"
     CDS             2397..>2661
                     /locus_tag="VF633_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8470814.1"
                     /translation="MGLIWLALAAIAVWALVRLGRQSEKLTNSGRAGGQWRIAATILG
                     AVLLAGAVLAGSHGAWLATAGMVGAGLYLIATSRVRPAATRPVR"
BASE COUNT          432 a          905 c          870 g          454 t
ORIGIN      
        1 gtcgtcgcgc agggtgggcg ccccgacgat ctccgtcggg atgtcgagat cggcgaccat
       61 gcggcgtacg accatcagct gctgccagtc cttttcaccg aagaccgcga catcggcctg
      121 cacctgattg agcagtttcg acaccacgag ggcgacgccg ccaaacatct gtggccggaa
      181 gtcgccctcc agtccctgag agggaccgcc cttcgacctg tcgccgacgg cgaccgaagt
      241 gacgaagccg tccggataca tctcggccac cgtgggggcg aacatcaggg cgcagccggc
      301 tccgctcagg agttccgcgt cgcgagcttc ctgccgcgga taggtcccca gatcctcgtg
      361 ggccgcgaac tgggtcggat tgacgaacac gctcgccact acgcggtctg cgcggcaacc
      421 cgcctcacgc acgagcgtta ggtgtccatc gtgcaaggcc cccatcgtgg ggacaaggcc
      481 gactgtcagt ccctgacggc gccactcgcg cacgatggcc cgcagttcgc taaccgtgcg
      541 cacaatgggg aggggttcgg ggttctcagc catggttact gcgttaacct tccgccttaa
      601 tcgtgttcgt gaaccggact tcaaagcctc gtccctaggg tggcgcttat gacgcccggc
      661 agaatgcttc gtccagccct ggtttgcctc gcactgtcgg ggatcggcct cacgacggcg
      721 tcgtgcggca tggtcgagag caatcctcac cggttcgaaa cgcttgcgga caaggttgcc
      781 gacatccagc tcgatgcgtc gggggaagtc ccgccgggga acatcacggt cccctcaacg
      841 ctgacagcgg cggaacagca tctgcgacct gcgacgtcgg cggcttccga accaccgtcc
      901 agaggcctgc gagtcgaggt catggatccc cacgatctgt gggacgcccg ggacggactg
      961 cgccgcacca tccgcgagga aagcaccgcc gtcgccgaag ccgctgcccc gatgatggct
     1021 cgcgccgtgg tccagcaggt ctcccaccgc ctgtccggat cgcctgcatc ggcggggcga
     1081 actgcccaac gcgccatcgt ccgtgaggtc gaagggcgca gtccggctca aacggtgatc
     1141 cagccccgcc agctgattca gcttggcgcc tattccagcg aggcttcggc ccggtctgcg
     1201 tggacgaggg tcagcaacgg cgcggcgcgt caggcgctca gcgggctctc gccgatgttc
     1261 gagcaagtca ctgtcggcgg ccgccccctg acccggctga aggttgcggc gccggcgact
     1321 gcggccaccg ccatctgccg cgccgtggcc atcagcgacc catggtgcgc acggcggacc
     1381 tgatgctggc cgcggaattc tgccgcccat catcccgtgc ggcgtgccgc cgtccacagc
     1441 tatgcgttaa cgctttgttg acgttcgtct aaaggcgccc gagtctggcg accattgacg
     1501 acgcgtgagt gagtcgtcgt ccagcaacag gtcaccgcat catggcgcag cctcaagtcg
     1561 tcgtcatcgg caatgaaaaa ggcggggcgg gcaagtccac cctggcgatc cacatcgtgg
     1621 tcggtctgct gcatgccggt caccgggtgg cgatcatcga cctggactcg cgccagcggt
     1681 cgatgtcgca tttcttctcc aaccgcgccg catggactgc agccaacggc cacaccctgc
     1741 cgatgccgat cgagcccgac atgggcgacg gcaaggcgct ggccaaggcg tctgaggaag
     1801 accagttggc gatcttcgag gccgcgtatc tgcaggctca ggactcagac atcatcgtca
     1861 tcgacacgcc gggcggggac acagccttgt ctcgcgccgc ccacggtcgc gccgaccaga
     1921 tcgtcacgcc gatgaacgac agcttcgtcg acttcgacat gctgggtcag gtcgatccgg
     1981 tgacgctcga cctgctcaag ccgtccacct attccgagag cgtgtgggaa gcccgcaagc
     2041 accgtgcgat caccgagggt cgtcacgcct cgatcgactg gctggtggtc accaaccgtc
     2101 tggccgtggc cgaggcccgc aatcgtcgcc gcctcgaaga gcggatgatg aagctggcca
     2161 agcgtgttgg ttttcgcgtc gggccgggcc tgcgcgaccg ggtcatctat cgcgagctgt
     2221 ttccgttcgg cctgaccgtc gccgacctgt ccaacgatat ccgccctgtt gccgtgtcgc
     2281 tggcccacgt cgctgcgcgt caggagatgc gcagcctgat gctggccatg ggtctggatc
     2341 aggcctcgct cgacgccaag gaacccgccc tggacgcggc agcctaagcc ttcaccatgg
     2401 gtctgatctg gctggcgttg gcggcgatcg ccgtctgggc gttggtgcgg ctgggccgac
     2461 aatccgagaa actgaccaac tccggtcggg cagggggcca gtggcgcatc gccgcgacta
     2521 tcctcggcgc ggtgctgctg gccggggctg tgctggccgg ctcgcacggc gcgtggctgg
     2581 ctacggcagg aatggtcggc gcgggcttgt acctgatcgc gacatcccga gttcgcccgg
     2641 cagcaaccag gccggtgagg c
//
LOCUS       DASVTM010000249         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_662163,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000249 DASVTM010000000
VERSION     DASVTM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662163"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VF633_06860"
     CDS             complement(<1..1475)
                     /locus_tag="VF633_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8470815.1"
                     /translation="MIRQTEAAECGLACLTMVARHHGQDIDLATLRRRFPVSIKGSTL
                     KSIMEIGSAIGLGSRAVRCEPDDLSDLKLPAILHWGMNHFVVLKRVRGNRLVILDPDA
                     GERRLTLGECSGFFTGVALELTPTDQFERKRDRQILKLSTLVRLSGSTVGVLVQAIVL
                     SLVIEAFVLATPFYMQLVIDEVVLKGDADLLTALAVGFAGLAIFSVAATVMRALTLQH
                     LSITLGFSMGANVFHHLIRLPLDWFHKRHTGDIQSRFRSLLPIQQFISNGAVTALLDG
                     VMSLATLALMFLYAPVLAGVVVASVAIYTAVRIASLGISRRVAGDYLINDAKEQTRFL
                     ETLRGAQTLKLAAAEPQRENLQRNAMAATLKSGQRAAHVNIGFQAVSQVLSGLTDILV
                     VFLAARAILSAEMTVGMLTAFMAYKGQFSGRVISFVENIINWRLLDVQLERLADIALT
                     PREPRIEDAETGGELIGHIEARRLVFRYSPGDPEILKGASF"
     gene            complement(1508..1978)
                     /locus_tag="VF633_06865"
     CDS             complement(1508..1978)
                     /locus_tag="VF633_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470816.1"
                     /translation="MKKCYALLLGLVTVTLLQSCSSAPRYAMRNLSSASIAIFVPGKE
                     GDRNSTYAMAGANSVSTPFALPVVPGTWAIRAGRCVHIYPTPQWQNPGDTERSLTVEE
                     RRILENWQRNLGTILNLSFSQDGKVRAYANDSGAEAGHEIEAAGFPMTPTSRCS"
     gene            complement(2038..2616)
                     /locus_tag="VF633_06870"
     CDS             complement(2038..2616)
                     /locus_tag="VF633_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470817.1"
                     /translation="MRELTLDEIEAVSGGVSLADLWNGLKSAFSSAGDFFGFDGHFGY
                     QGGDGGLSFGGFGSAVGEAAGYAWAAPNTAIGLVIGGLGWAYDHTFGDHVSGVEITNG
                     VIQFTDNPFLSSPGDFNDGAITFGHVQIYGDNTASAVHEMQHTYQYEQWGPFFLPAYV
                     LSGFGAMIQGGSFIGSGNFFESGPYSNPPTPY"
     gene            complement(2679..>3689)
                     /locus_tag="VF633_06875"
     CDS             complement(2679..>3689)
                     /locus_tag="VF633_06875"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX8470818.1"
                     /translation="HVRRGQPIALIGTEQLLPGGGALDEDILASVETESSMLAVQLES
                     LEATAPLEIATLVSERERLVTERNAAGAQAEAAKARLGLARERLDTAEGLASRGYLAG
                     DQLRQRRSDVIAMEESRASATEQVRGLGAQISGLDARIAKAPHDARAARAQLQGKASQ
                     LTQTRAQIAGRRGYELRAPSDGRITALQAGLGQIVDPTKPLMTLTPMNGRLRAELYVP
                     SRAIGFIRPGQRVRLLYDAYPYQKFGPAWGRIIEISATVLGPQEIAAVIQTSEPVYRV
                     VAQMDRQSMQAFGEPRPLSAGMALTADIILEERTFAEWLLEPLLAMRGRTQQPPESAA
                     SS"
BASE COUNT          713 a         1151 c         1133 g          692 t
ORIGIN      
        1 tcgaaactgg cgcccttcag gatctcggga tcgccggggg aatagcgaaa caccaggcgg
       61 cgcgcctcga tgtggccgat gagttcgccg cctgtttcgg cgtcctcaat cctgggctca
      121 cgcggggtca gggcgatgtc cgccagtcgc tcaagctgca cgtccagcag gcgccagttg
      181 atgatgttct cgacgaagct tatcacgcgc ccggagaact gacccttgta ggccataaag
      241 gcggtcagca tgccgacggt catttccgcc gagaggatcg cgcgggcggc caggaaaacc
      301 accaggatat cagtcaggcc tgacagcacc tgagacacgg cctggaaacc gatattgacg
      361 tgcgcggcgc gctggcccga cttcagggtg gccgccatgg cgttgcgctg aaggttttcg
      421 cgctggggtt cggcggcggc cagtttcagc gtctgcgccc cccgtagcgt ctccaggaac
      481 cgcgtctgct ctttcgcgtc attgatcagg tagtcgcccg cgacacgtcg cgagatgccc
      541 agactggcga tccgcacggc ggtgtagatt gcgacgctgg cgacgaccac gcctgccagg
      601 accggcgcgt agaggaacat cagggccagc gtcgccagac tcataacgcc gtccagcaag
      661 gccgtcacgg ccccgttgga gatgaattgc tggatcggga gcagggacct gaaacggctc
      721 tggatatcgc ccgtatgacg cttgtggaac cagtccagcg gcaggcggat caggtgatga
      781 aagacattgg ctcccatgga gaaccccagg gtgatggaca ggtgctgtag ggtcagcgcc
      841 cgcatgacgg tggccgcgac cgagaagatc gcgagcccgg cgaagcccac cgccagggcg
      901 gtcagcagat cggcgtcgcc tttcagaacg acttcgtcga tgaccagctg catgtagaag
      961 ggcgtggcca ggacgaaggc ctcgatgacc agggacaaga caatcgcctg gaccagcacc
     1021 ccgaccgtcg agcccgaaag gcgcaccagc gtcgacagtt tcaggatctg acggtcgcgc
     1081 ttccgttcga actggtcggt aggcgtcagt tccagggcca ccccggtgaa gaatccggag
     1141 cattcgccca gcgtcagccg tcgttcgccc gcgtcagggt ccaggatcac gagccggttg
     1201 ccccggacgc gcttcaggac gacgaaatgg ttcatgcccc aatgcaggat cgcgggcagt
     1261 ttgaggtcgg acaggtcgtc cggttcgcag cgaacggccc gcgaacccaa accgatcgca
     1321 ctgccgatct ccatgatcga cttcagcgtc gaccccttga tggaaacggg aaaccgccgt
     1381 ctcaacgtcg ccagatcgat atcctgacca tggtggcgag ccaccatggt caggcaagcc
     1441 agtccgcatt cggcggcttc agtttgtcga atcatcagga ttcaggtttg gctttccgga
     1501 gacttgatca gctgcagcgg gatgtgggtg tcatggggaa tcccgccgcc tcgatctcat
     1561 gtccggcctc tgccccactg tcattcgcgt aggcacggac cttgccatcc tgggaaaacg
     1621 acaggttcaa tatcgtcccc agattgcgtt gccagttctc caggatcctg cgttcctcaa
     1681 ccgtgagaga acgttcggta tcaccgggat tctgccattg cggtgttgga tagatgtgga
     1741 cgcaacgccc cgcgcgtatc gcccaggttc caggtacgac gggaagagca aatggagttg
     1801 agacggagtt ggccccggcc attgcgtagg tcgaatttcg atcgccttcc tttccaggga
     1861 caaagatcgc tatcgatgcc gaagacaaat tacgcatcgc gtatctgggc gcagatgagc
     1921 aactctgaag tagagttacg gtcaccaggc ccaaaagcaa ggcataacac tttttcatcc
     1981 gagactcctc gttgttcccg aagctaagtt aaatcgttcc gctcaggcgg cttctattta
     2041 gtaaggtgtt ggtggattgc tataaggtcc ggattcgaag aagtttcctg aaccgatgaa
     2101 gcttccgcct tgaatcatgg cgccgaagcc gctcaacaca taggctggca ggaagaacgg
     2161 cccccattgt tcgtattgat aggtgtgctg catttcgtga actgcactcg ccgtattgtc
     2221 tccatatatc tggacgtggc cgaatgttat ggctccatcg ttgaaatcgc cgggagaaga
     2281 cagaaatgga ttgtcggtaa actggatcac cccgttggtg atttcgacgc cggaaacgtg
     2341 atcgccgaac gtgtgatcgt aagcccagcc caagcctccg attaccagtc caattgccgt
     2401 attgggagcg gcccaggcgt acccggccgc ttcaccgacc gcacttccaa acccgccaaa
     2461 gctcagtccc ccgtcgccac cctgatagcc gaagtggccg tcgaagccga agaagtcccc
     2521 ggcgctgctg aacgcgcttt tcaaaccgtt ccagaggtcg gccagcgaca ccccgcccga
     2581 aaccgcctcg atctcatcca atgtcagctc acgcatctgt ctttctcccc cgctcgtgat
     2641 cgccaccggc ttcgcgggaa agccggtccg aggcgttgtc aggatgacgc cgccgattcc
     2701 ggtggctgct gcgtccgtcc acgcatcgcc agaagaggtt caaggagcca ttccgcgaag
     2761 gtgcgctcct ccagaatgat atcggcggtc agggccatac cggccgaaag gggccggggc
     2821 tcgccgaagg cctgcatcga ctggcggtcc atctgcgcca cgacgcggta gacaggttcg
     2881 gatgtctgga tgaccgccgc gatttcctga ggccccagga ccgtcgccga tatctcgatg
     2941 atccgccccc aagcgggccc gaacttctga tagggatagg cgtcatacag caggcggacc
     3001 cgctggccgg ggcggatgaa cccaatggcg cgagatggca catacagttc ggctcgcaac
     3061 cgaccgttca tcggcgtcag cgtcatcaga ggcttcgtcg gatccacgat ctgacccaag
     3121 ccggcctgga gcgccgtgat ccggccgtcg gaaggcgcgc gaagttcata acccctgcgc
     3181 ccggcgatct gggcgcgggt ctgagtcaac tgagaggcct tcccttgcag ctgagcccgg
     3241 gccgcacggg cgtcgtgcgg cgccttggcg atccgggcgt ccaatcccga gatttgcgca
     3301 cccaagcctc gaacctgttc ggtggcgctg gcccgcgatt cctccattgc gatcacgtcg
     3361 ctgcgccgtt ggcgcagctg atctccagcg aggtatccgc gggacgccag gccttcggcc
     3421 gtatccagtc gttctcgcgc aaggcccaac cgggccttgg ccgcctcggc ctgggcgcct
     3481 gcggcgttgc gctctgttac cagtcgttcg cgttccgaaa ccagggtcgc aatttcgaga
     3541 ggcgccgtcg cttcgaggct ttctaattgg acagccagca tcgacgactc cgtctcgacg
     3601 ctggccagga tatcctcgtc gagagcgccg ccgccgggca gaagctgttc ggtgccgatc
     3661 agcgcgatgg gctggccgcg cctgacatg
//
LOCUS       DASVTM010000250         6166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_292394,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000250 DASVTM010000000
VERSION     DASVTM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292394"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF633_06880"
     CDS             complement(<1..465)
                     /locus_tag="VF633_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8470819.1"
                     /translation="MTAFPTRAYDAFLFDMDGTLITSIAAAERVWSAWATRHRLDLDV
                     FMPTIHGVRAIDTIRNQNLPGIDLQTEVAWVTRSEIEDTDGVAEIPGAIAFVATLPPE
                     RWAVVTSASPELAEARLRAAGLTPPRLVITAHDIERGKPDPEGFRLAAERLGF"
     gene            complement(525..1385)
                     /locus_tag="VF633_06885"
     CDS             complement(525..1385)
                     /locus_tag="VF633_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8470820.1"
                     /translation="MSRTHAAAAILISALTFTASIAMADDRGADDRASQTAATGPVPA
                     TAQQRADSERLDAVTRSVFWNREREVNPADPVAGVKLAQALRELGQFDQAAETAQSVL
                     TVQPDNVEAMLELGKDHIARAQGFYGIAALEQARALAPRDWRPLSLLGVAYTQVRRED
                     DAKAAWNEGLRLSADNPEILTNAAVALMTAGNLPGAEGLLRRAVAQPAATARMRLNLA
                     MALGLQGKMPEAEQIIRRDLPPEQADSNLQWLRSKAAPGAVMPASMVADPVQATGSAT
                     DRTWNSLQGQ"
     gene            1474..2862
                     /locus_tag="VF633_06890"
     CDS             1474..2862
                     /locus_tag="VF633_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX8470821.1"
                     /translation="MSAAHAPVLISAAEAAVPVRLVADDTTLEGRAAAWAAANSFTGK
                     TGQMLLVAGEDGALSEVLFGMGGAFDPKSLRAFSAKLPAGTYRLEGVEAADAGAAALQ
                     FVLGAYLFDRYKARPERGGVHLVAPDGVDVAETLTIASACALAREMVDTPAADMGPLQ
                     IETIAREIAESAGATISLIEGEALLEENYPAVHAVGRAAAPHRQPRVIEIGWNLDRTD
                     LPLVALVGKGVVFDTGGLDLKPAAGMRNMKKDMGGSAHALALARLVMSAGLAVRLVVL
                     VAAVENAVSADAFRPGDVLNSRKGLTIEIGNTDAEGRLILADILTRASEHSPDLTLDF
                     ATLTGAARVALGPELPPLYTDDEALATDLLSASAAVDDPLWRMPLWAGYKASIDSEIA
                     DVRNDSAAWAQAGSVTAALFLQRFAPTTGAWAHMDIFAWNSRARPGHPEGGEAQAMRA
                     CYEMLKRRYPKA"
     gene            complement(2847..3329)
                     /locus_tag="VF633_06895"
     CDS             complement(2847..3329)
                     /locus_tag="VF633_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470822.1"
                     /translation="MRLSLLVGILATVIVFPETPIARTLHRHLVERVAQGMNRVRLAH
                     IVFGAALTAIGMVFIVVFEGEGLRVFGMMAPEAVSWFAMFDVALFLDVFAMTLALAAT
                     TRLKAARDQIIAGIRLVPGHIRSALSSGRRRARRDKSTTVRPKRSDDPDPFGAGYAFG
                     "
     gene            3497..4303
                     /locus_tag="VF633_06900"
     CDS             3497..4303
                     /locus_tag="VF633_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX8470823.1"
                     /translation="MNASAAIIERSWLVRADLAEQGLEGLVRAPAFKAVLPMHGRVPI
                     ADIFAEAAGSERIDQLIFGEAFDALDRHTTAEGADRVWGRARRDGVVGWIDRAALRPG
                     APLATHRVASTSAVLPLNALIHHGWSGVAADDLMIAGEFERDPVAVAERMLDVPHELG
                     ARTSRATDCSGLVQQALYACGLAGPRRSDEQAELGVAVTRSELQRGDLVIWLHADTGQ
                     SWTGHSALALDAEHLIHATGHAGRVVVEDLAEAEARYVADGFSPPVFRRL"
     gene            complement(4369..5121)
                     /locus_tag="VF633_06905"
     CDS             complement(4369..5121)
                     /locus_tag="VF633_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX8470824.1"
                     /translation="MSGDLKWNKIFGAGLATAFVIIGVQQVATHVYATHAPEKPGYFV
                     NAPEEAAAGDEAPALLPDWGTVLPAADLAAGEAAFARCKACHNNAAGGADGIGPNLYG
                     VVGGPVMHRAGFAYSDAMTKHKGEKPTWGYDELDQFITAPQRYVPGTKMSFAGLRDTP
                     TRINLIAYLRAQGSTGFAIPAPDPARQPGAAPAAAGAAAPGAEGATAPDAAAGTPASG
                     PTVPVTGPAGASTTQATPQAPPASTSQAPSNH"
     gene            5270..5992
                     /locus_tag="VF633_06910"
     CDS             5270..5992
                     /locus_tag="VF633_06910"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX8470825.1"
                     /translation="MNPLVLIPTRMAATRLPNKPMADIGGKPMIVRAWESALNSGLQV
                     AVAAGDQEIVDVIVALGGQAVLTDPDLPSGSDRIRAAVEAIDPDGEYDAIINLQGDMP
                     FADPGLATACAAVLNGEPTCDIATLVAAESDISDRTNPDVVKAVLALADGERQAKALY
                     FTRSTLYGDAPVWRHIGVYGYRRDALMRFCAAPPSPLEKREKLEQLRALELGLSIWAS
                     VIGEAPLSVDNPADLEAARKLA"
BASE COUNT          977 a         2071 c         2056 g         1062 t
ORIGIN      
        1 gaaacccagc cgttcggcgg ccagccggaa accttccgga tcaggcttgc cgcgttcgat
       61 gtcatgggcg gtgatcacga ggcgaggcgg cgtcaatccg gctgcccgca gtcgggcctc
      121 ggccagctca ggagacgccg atgtgaccac ggcccagcgc tctggcggca gcgtcgcgac
      181 gaaggcgatg gcgccaggga tttccgctac gccgtcggta tcctcgatct cgctgcgcgt
      241 gacccaggcg acttcggtct gcaggtcgat gccgggcagg ttctggttgc ggatggtgtc
      301 gatcgcccgg acgccgtgga tcgtcggcat gaagacgtcg aggtcaagcc ggtggcgtgt
      361 ggcccatgcc gaccagaccc tctcggccgc ggcgatggat gtgatcagcg tcccgtccat
      421 gtcgaacagg aaggcgtcgt aggcgcgagt tgggaaggcg gtcatggggt tcatccagcg
      481 cgcgcctgac aacacggcaa atgacaggca gttactatgg ccgcctattg gccctgtagc
      541 gagttccacg tccggtcggt tgccgaaccc gtcgcttgca ctggatcagc caccatcgac
      601 gccggcatca ccgcccccgg cgccgccttg gatcgcagcc attgcagatt gctgtcggcc
      661 tgttccggcg gcaagtcgcg gcggatgatc tgttccgctt ccggcatctt gccctgcagg
      721 cccagcgcca tggcaaggtt cagccgcatc cgtgcggtgg cggcgggctg ggccacggcg
      781 cggcgcagga ggccttcggc gcccggcagg ttgccggcag tcatcagcgc gacagcggcg
      841 ttggtcagga tctcggggtt gtctgccgac aggcgcaggc cctcgttcca ggcggcttta
      901 gcgtcatctt cgcggcggac ctgggtatag gccacaccca gcagtgacag tggccgccag
      961 tcgcgggggg cgagcgcgcg agcctgttcc agcgccgcaa tgccgtagaa gccctgagcg
     1021 cgcgcgatgt gatccttgcc cagttccagc atggcctcga cgttgtcggg ctggacggtc
     1081 agcacggatt gcgcagtctc tgcggcctga tcgaactggc cgagttcacg cagggcctgc
     1141 gccagtttca cgcctgccac cgggtcggcc ggattgacct cacgctcccg gttccagaac
     1201 accgatcgcg tcacggcgtc cagccgttcg ctgtctgcgc gctgctgggc cgttgcgggc
     1261 acagggccgg tcgcagccgt ttgcgatgcg cgatcatcag ccccccgatc atcagccatg
     1321 gcgatgctcg ccgtgaacgt cagggcgctg atcaggattg cggcggcggc gtgcgtgcgc
     1381 gacatggagg catccggtgg aaatctatcg cgccgagaat cccgctttgc cgtcaagcat
     1441 tggttaatat ccgccgctcg tctcgaggtc cccatgtccg ctgctcacgc ccccgtcctg
     1501 atctccgccg ccgaagccgc cgtcccggtt cgtctcgtgg ccgacgacac aaccctcgaa
     1561 ggccgtgccg ccgcctgggc cgccgccaac agcttcaccg gcaagacggg gcagatgctt
     1621 ctggtcgcag gtgaggacgg cgccttgtca gaagttctgt tcggaatggg gggagctttc
     1681 gatccgaagt cgctccgggc gttttctgcg aaattgcccg ccggaaccta caggctggaa
     1741 ggcgtcgagg ctgccgacgc cggggctgcg gcgctgcagt ttgtgctcgg ggcctatcta
     1801 ttcgatcgct acaaggcccg gcccgagcgg ggcggggtgc atctggtcgc gccggacggc
     1861 gtcgacgtcg cggagacact gaccatcgcc tcagcctgtg ccttggcccg cgagatggtc
     1921 gacacgcccg ccgccgacat gggcccgctg cagatcgaga caatcgctcg cgagatcgcc
     1981 gagagcgcag gggccacgat ttcgctgatc gagggcgagg ctttgctgga agagaactac
     2041 ccggccgtcc acgccgtcgg tcgcgccgct gcgccgcatc gccagccgcg tgtgatcgag
     2101 atcggctgga atctcgatcg gacagacctg ccgctggtgg cgctggtcgg caagggcgtg
     2161 gtgttcgaca ctggcggtct cgacctcaaa cccgccgccg ggatgcgcaa catgaagaaa
     2221 gacatgggcg gctctgccca tgcgctggcg ctcgcacggc tggtgatgtc ggcgggtctg
     2281 gccgtgcggc tggtggtgct ggtcgcggcg gttgaaaacg ccgtgtcggc cgacgccttc
     2341 cgccccggtg acgtgctgaa cagccgcaag ggtctgacca tcgagatcgg caatacggat
     2401 gccgagggcc ggttgatcct cgccgatatc ctgacccgtg catccgagca ctcgcccgac
     2461 ctgacgctgg atttcgccac cctgacgggc gctgctcgcg tggcgcttgg gcccgaattg
     2521 ccgccgctct acaccgacga tgaggctctc gcgaccgacc tgctcagcgc ctccgcagct
     2581 gtagacgacc ccctgtggcg gatgccgctg tgggccggct acaaggcctc aatcgacagc
     2641 gagatcgccg atgtcaggaa tgattccgcc gcctgggctc aggctggatc ggtgaccgcc
     2701 gccctgttcc tgcaaaggtt cgctccgacc accggcgcct gggcgcacat ggacatcttt
     2761 gcctggaaca gccgcgcccg gccgggacac cccgagggcg gcgaggcgca ggccatgcgt
     2821 gcctgctacg agatgctcaa gcgccgctac ccgaaggcgt agcccgcgcc gaacgggtcg
     2881 gggtcgtctg agcgcttcgg gcgcacggtc gtcgacttgt cgcggcgcgc ccgccgccga
     2941 ccgctgctca gggctgagcg aatatggccc ggaacgagcc tgatcccggc gatgatctgg
     3001 tctcgcgcag ccttcaggcg ggtcgtggcg gcgagggcca atgtcatggc gaagacatcg
     3061 aggaacagcg ccacgtcgaa catcgcaaac cagctcacgg cctccggcgc catcatgccg
     3121 aacactctga gaccctcgcc ctcgaagacc acaatgaaca ccatgccgat cgcggtcagg
     3181 gccgccccga agacgatgtg agccagccgc acccggttca tcccttgcgc aacacgctcg
     3241 accagatgcc ggtgcaaggt ccgcgcgatc ggcgtttccg gaaagacaat cacggtcgcg
     3301 aggatcccca ccaacaggct cagccgcatc gaaaatcatc cctcgttcgg tcaacatcga
     3361 tctgggaacg atgcggccac gcttcaagat gccgctataa tccggtggaa aagtcgcggc
     3421 tgttaacgtc gctttcggct tcttcggcga tggtggaggc cggctcgtca aggagccttt
     3481 gcacgtcggg attcgcttga acgcatccgc cgccatcatt gagcgttcct ggctcgttcg
     3541 tgccgatctg gccgaacagg gtctcgaagg gctcgtgcgc gcgccggctt tcaaggccgt
     3601 gctgccgatg cacggccgcg tgcccatcgc cgacatcttc gctgaggccg ccggttccga
     3661 gcgtatagac cagctcattt tcggtgaagc cttcgacgcg cttgaccgcc ataccaccgc
     3721 agagggagcc gaccgcgtct ggggtcgcgc ccgccgtgac ggggtcgtcg gctggatcga
     3781 ccgggccgct ctcagacccg gtgcacctct ggccacgcac cgtgtcgcgt ccaccagcgc
     3841 ggtcctgccg ttgaacgccc tgatccatca tggctggtcc ggcgtcgccg ccgacgacct
     3901 gatgatcgcc ggagagttcg agcgcgatcc tgttgcggtc gccgaacgga tgctcgacgt
     3961 tcctcacgaa ctcggcgccc gcacttcgcg cgccaccgat tgctcggggc tggtgcaaca
     4021 agcgctctat gcctgcggtc ttgccggacc gcgccggtct gatgaacagg ccgaactggg
     4081 cgtcgccgtc actcgcagcg agctgcaacg cggcgatctc gtcatctggc tgcatgcaga
     4141 caccggccag agctggaccg gtcattccgc tctggcgctc gacgccgaac acctgatcca
     4201 cgctaccggc catgccggcc gcgtcgtcgt cgaagatctg gctgaggccg aggctcgcta
     4261 tgtcgccgac ggcttttcac cgcccgtctt ccggcgtttg tagcctcgcg caaaagaaag
     4321 ggcggtccga aaaccgccct cccaaaacag ttgaacttac cgaaccgtct agtggttgga
     4381 cggcgcctgg cttgtcgatg ccggcggcgc ctgtggcgtt gcctgggtgg tcgaggctcc
     4441 ggcgggaccg gtgacgggca cggtcggccc ggaagccgga gttcctgcgg ccgcatcggg
     4501 agcggtcgcg ccttcagccc ccggagcagc ggcgccggcg gcagcgggcg ctgcgcccgg
     4561 ctgacgcgcg ggatccggcg ccggaatggc gaacccggtc gaaccctgcg cgcgcagata
     4621 ggcgatcaga ttgatgcggg tcggggtgtc acgcagaccg gcgaaggaca tcttggtgcc
     4681 gggcacatat ctctgcggcg cggtgatgaa ctggtccagt tcatcatagc cccacgtagg
     4741 cttctcgccc ttgtgtttgg tcatggcgtc cgaataggcg aagcctgcgc ggtgcatgac
     4801 cggaccgccg accacgccgt acaggttcgg accgatcccg tcggcgccgc ccgcagcgtt
     4861 gttgtggcag gccttgcagc gcgcgaacgc ggcctcgccg gcggccaagt cggcggcggg
     4921 caggaccgta ccccagtcgg gcaggagggc aggggcttcg tcgcccgcag cagcctcttc
     4981 aggcgcgttg acgaaatagc cgggcttctc aggcgcgtgc gtcgcataga catgcgttgc
     5041 gacctgctgg acgccgatga tgacgaacgc cgtcgccagg cccgcaccga aaatcttgtt
     5101 ccacttcaga tcgccgctca tccgcgtcgt cgcatcccgt agctgggccc cgtcttgaga
     5161 tcgacgaggc gaattcttgc gtgggcgtct ctgacacgct agcgccgggt tcgcaaccgg
     5221 cccacgacgc agtttggagc gtctcgccgt tacaaggcaa cactcgacga tgaacccgct
     5281 cgtattgata ccgactcgca tggctgcgac ccgtctgccg aacaagccaa tggccgatat
     5341 cggcggcaag ccgatgatcg tgcgggcttg ggagagcgcc ctgaattccg gcctgcaggt
     5401 cgccgtggcg gcaggtgatc aggaaatcgt ggatgtcatt gtcgctcttg gcggccaggc
     5461 cgtgctgacg gatcccgacc tgccctcggg ctcggaccgt atccgtgcgg cggttgaggc
     5521 gattgatccc gacggcgagt atgacgccat catcaacctg cagggcgaca tgccgtttgc
     5581 cgatccgggc ctcgccaccg cctgcgccgc cgtgttgaac ggcgagccga cctgcgacat
     5641 cgctacgctg gtggcggccg aatcggacat ctcggaccgc accaacccgg atgtcgtgaa
     5701 ggccgtcctc gcgctggctg acggcgagcg tcaggccaag gccctctatt tcacccgctc
     5761 caccctgtac ggcgacgccc cggtctggcg tcacatcggc gtctacggct accgccgaga
     5821 cgcgctcatg cgcttctgcg cggccccgcc gtcaccgctc gaaaagcgcg agaagctgga
     5881 acaactgcgg gcgctggagc tgggcctcag catctgggcg tcagtgatcg gcgaagctcc
     5941 gctgtcggtc gacaaccctg ccgatctgga agcggcccgc aaactggcgt gacgtttcac
     6001 ctccccatcg ccgaagggcg atggggagga cagatcgcgt gagcgatcag gagggggcga
     6061 caccgtgcca gccggtgagc catcttcaat accgagtagc cctctcctga tcgtcgcgat
     6121 gcgccgatct gtcctctcca ttcgccttcg cgaatggaga ggtgga
//
LOCUS       DASVTM010000251         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1034114,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000251 DASVTM010000000
VERSION     DASVTM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034114"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VF633_06915"
     CDS             complement(<1..781)
                     /locus_tag="VF633_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8470826.1"
                     /translation="MMKLPCLIAAALLAAPSLSVAQTPAAADRPANSARLEGYEYPYP
                     VQVWAFESQRKPLEMTYMDVAPTGLANGRTAVLLHGKNFCAATWAPTIKVLSEAGYRV
                     IAVDQIGFCKASKPEAYQFSLAQLAENTEGLLKHLGIERVSLIGHSMGGMLAARFAIQ
                     NPALVERLVLVNPIGLEDWQAEGVPYASLDDAFAGEQRTTFDSIKAYQQKFYYGGEWR
                     PEYDPPVAMLAGMYAGPGRDAVAWNQAQTSDMIFTQPVVHEF"
     gene            complement(835..1968)
                     /locus_tag="VF633_06920"
     CDS             complement(835..1968)
                     /locus_tag="VF633_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX8470827.1"
                     /translation="MTDTTAAPETSEPANTQAPATAPAPGSAVASEPKKRVLFAVALG
                     LIALLGITLILYAWKLPPFTGAIQRTDNAFVRGQVTIISPQVSGYVTSVRVQDYQVVK
                     QGELLATVDDRIYRQQLEQALANLHSAEASLANSANTQASAQGTVAQRRASIASAEAS
                     LVQAQANANRARKLFEGGWVAQAQIDTTSASLRAAQAAVAEANALQGVAQTGVSSAVV
                     SRGSLEAAVENARAQVQLAQINLDNTRIIAPADGRLGEVAVRLGQSVVAGSTQLTSLV
                     PNLIWVNANMKETQLKNIRIGQPVVMWVDSLGGAELRGHVERISPATGSEFSVIRPDN
                     ATGNYTKVAQRVPVRIAIDPNQELATRLAPGMSVVAKIDTSGT"
     gene            complement(1978..3654)
                     /locus_tag="VF633_06925"
     CDS             complement(1978..3654)
                     /locus_tag="VF633_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8470828.1"
                     /translation="MTRPHDLPWEEYEKTLQPHEKPTLMGSPATPDHHWRLRAAYGAI
                     GVLVAMTGGLGAGVVSANIQSLAGHLGVTTTEAAWLPVVYVMTNACMNLLLVKFRQQY
                     GLQPFTRIMLGIFVLVSIGNVFASTYQSELLTRAVSGICGAGMSSLGFLYLIQAFPAA
                     HRLKGLVIGIGLSSFALPIARITSSTLLDIAGWEGLYLAELGLALLSLAAVFSLRLPP
                     SQRSQVYQPLDFLTFALFAPGIALLCAVLGLGRLVWWTEAAWIGWALIGAVVLLATAL
                     LVEFYRKKPLLDLKWLGGGDLIRLLLAILLIRVVLSEQTSGSVGFLQQMGLGLDQLHA
                     LFWVILVAMIAGTATSALSLNMAKLYKPIAIALALIAIGTFIDSHATVLTRPANLFFS
                     QALLAFASALFIGPALMIGIVQVLSRGPQFLVSFIVTFSVVQNVGGLAGGALVGTVET
                     IREKYHSSQLAESITLGDPQVVLRLQQLGGAYARVQNDTALRTADGISLLSQQVTQQA
                     HVLAYNDVFLMISIAAALGCVWTTFVHFRPHIQARRAAARDAATSAASAH"
     gene            complement(3651..4205)
                     /locus_tag="VF633_06930"
     CDS             complement(3651..4205)
                     /locus_tag="VF633_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8470829.1"
                     /translation="MARAPRKDAIERRGALLAAAAEEFALHGYNVPLDAIADRAGVGR
                     ATLYRNFADRTALALAVFEEQLEDLAARTRERGDDPEAFFWFVDQLADVLGRNAGLDA
                     ALREAQSSGALKPIRLKLVEAGESALERAKAAGLVRADLGPSDIRAIAMILGAGASRP
                     TPEERDMFGQRIRALLLDGLRTRS"
     gene            complement(4306..>4656)
                     /gene="rlmJ"
                     /locus_tag="VF633_06935"
     CDS             complement(4306..>4656)
                     /gene="rlmJ"
                     /locus_tag="VF633_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX8470830.1"
                     /translation="DLILIDPPFEQPDDYLRAAETTLELVKSRPGVTVAIWTPLKDLE
                     TFDGFLRRLGTTIPTVVAEVRLRPLTNPMKMNGCAMVVVNPPAGTDEAAREICSWVAE
                     TLGETGQKSDIWRL"
BASE COUNT          880 a         1456 c         1540 g          780 t
ORIGIN      
        1 cgaactcgtg gaccaccggc tgggtgaaga tcatgtccga ggtctgggcc tgattccagg
       61 ccacggcgtc gcgtcccggt cctgcgtaca tcccggccag catcgcgacc ggcggatcat
      121 actcgggacg ccattcgcca ccgtaataga acttctgctg gtaggccttg atgctgtcga
      181 aggtcgtgcg ctgctcgccg gcgaaggcgt cgtccagcga ggcgtacggc accccctcgg
      241 cctgccagtc ctccagtccg atcggattga cgagcaccag acgctcgacc aaggccgggt
      301 tctggatagc gaacctcgcc gccagcatcc cgcccattga gtgaccgatc agcgacacgc
      361 gctcgatgcc cagatgcttc agcagccctt cggtgttctc ggcgagctgg gcgaggctga
      421 actgataggc ctccggcttg gacgccttgc aaaagccgat ctggtcgacg gcgatgaccc
      481 ggtatcccgc ttcggacagc accttgatgg tcggcgccca ggtcgcggcg cagaaattct
      541 tgccgtgcag caggaccgca gtccgaccgt tcgccaagcc tgtcggtgcg acgtccatat
      601 aggtcatctc cagcggctta cgctggcttt cgaaggccca aacctgaact ggatagggat
      661 attcgtagcc ctcgaggcgc gcactgttgg ccgggcgatc agccgcagcg ggcgtctgag
      721 cgacggacaa tgaaggcgca gccaacaggg cggcggcaat caggcaagga agcttcatca
      781 tacgaactca acctgtgtcg tccagggacg ttcctttccg cgacgacgtg tccttcaggt
      841 cccggaggtg tcgatcttcg ccaccaccga catgcccgga gccagtcgcg tcgccagttc
      901 ctgattggga tcgatggcga tgcgcacggg cacccgttgg gcgaccttgg tgtagttgcc
      961 ggtggcgttg tcaggcctga tgacgctgaa ctccgaacca gtcgccggcg agatgcgctc
     1021 aacatgaccg cgcagttcgg cgccgcccag agagtcgacc cacattacga ccggctggcc
     1081 gatgcggatg ttcttcagct gggtctcttt catattggcg ttgacccaga tcaggttggg
     1141 caccagcgag gtcagctggg tgctgcccgc caccaccgat tgaccaaggc gcaccgcgac
     1201 ctcgccgaga cgaccgtcgg cgggggcgat gatgcgggtg ttatcgagat tgatctgcgc
     1261 cagttgcact tgagcgcgcg cgttctcgac agcggcttcc agcgagccac ggctgacgac
     1321 cgccgacgag actccggtct gggccacgcc ctgcagggca ttggcttcgg cgacggcggc
     1381 ctgtgcggcg cgcagggagg ctgaggtggt gtcgatctgg gcctgcgcga cccagccgcc
     1441 ttcgaacagc ttgcgtgcgc ggtttgcgtt ggcctgggcc tgcaccagag acgcttccgc
     1501 agaggcgatc gaggcccggc gttgggcgac tgtgccctga gccgaagcct gggtgttggc
     1561 ggagttggcc agcgaggcct cggcggaatg gaggttggcc agcgcctgct ccagttgctg
     1621 gcgatagatg cggtcatcga cggtggccag cagttcgccc tgcttgacca cctgatagtc
     1681 ctgcacccgc accgaggtga catagcccga gacctgcgga ctgatgatgg tcacctgacc
     1741 gcgcacgaag gcattatcgg tgcgctggat ggccccggtg aacggcggca gcttccatgc
     1801 atagaggatc agcgtgatgc ccagcagggc gatcagcccc agcgccaccg cgaacaggac
     1861 gcgcttcttc ggctcagacg caacggctga accgggtgcc ggagccgtag ccggagcttg
     1921 cgtgttggcg ggttcagagg tctcgggggc ggctgtagtg tcggtcatgg tcggatctca
     1981 gtgagcggat gcggcggacg tggccgcatc acgcgcggcg gcgcgacgcg cctgaatatg
     2041 cggtcggaaa tgcacgaaag tggtccagac gcagccgagg gcggcggcaa tggaaatcat
     2101 caggaaaaca tcgttgtaag ccagcacgtg agcctgctgg gtcacctgct ggctaagcag
     2161 tgagatgccg tcggccgtcc gtagggccgt gtcattctgg acgcgggcat aggctccgcc
     2221 cagttgctgg agacgcagca ccacctgcgg atcgccgagg gtgatgctct cggccagttg
     2281 gctcgagtgg tatttctcgc ggatcgtctc cacggtgccg accagcgcgc cgccggccag
     2341 accaccgacg ttctgcacca ccgagaaagt gacgatgaag ctgaccagaa attgcggccc
     2401 ccgggacagg acctggacga tgccgatcat caacgccggg ccgatgaaca gggccgaggc
     2461 gaaggccagc aaggcctggc tgaagaacaa atttgccggg cgggtcagaa cagtagcgtg
     2521 gctgtcgatg aaggtcccga tggcgataag ggccagggcg atcgcgatcg gcttgtagag
     2581 cttcgccatg ttcaagctca gcgcactggt cgccgtgccg gcaatcatgg ccaccagaat
     2641 gacccagaac aaggcgtgca gttggtccag cccgagaccc atctgctgca ggaaaccgac
     2701 ggaaccggac gtctgttcgg acaggaccac gcggatcagc aggatcgcca gcagcagccg
     2761 gatcaggtcg ccaccaccca gccacttcaa gtcgagaagc ggcttcttgc ggtagaattc
     2821 gacgagcagc gccgtcgcca gcagcacgac cgcccctatc agggcccagc cgatccatgc
     2881 cgcctcggtc caccagacca gccgtccaag ccccagtacc gcgcacagta gggcgatgcc
     2941 cggggcgaac agcgcaaagg tgaggaagtc gagcggctga tagacctgcg acctttgcga
     3001 cggcggcaga cgcaggctga agacagccgc cagcgacagc agggccagac ccagctcagc
     3061 cagatacaga ccttcccagc cggcgatatc aagaagagtg ctggaggtta tccgggcgat
     3121 cggcagggcg aaactggaca ggccaatgcc gatcaccaac cccttgagcc ggtgcgccgc
     3181 cggaaaggcc tggatcagat agaggaagcc gagcgacgac atgccggccc cgcagatccc
     3241 ggacacggcc cgggtcagca gttcggactg ataggtgctg gcgaagacat ttccgatcga
     3301 gacgagcaca aagatgccca gcatgatgcg ggtgaaaggc tgcagcccgt actgctggcg
     3361 gaacttcacc agcaacaagt tcatacaggc gttggtcatg acatagacca ccggcagcca
     3421 ggcggcctca gtggttgtaa cgcccagatg accggccagg ctctggatat tggcgctcac
     3481 cacaccggcg cccaacccgc ccgtcatcgc caccagaacg ccgatggcgc cataggcggc
     3541 gcgcagacgc cagtgatggt ccggcgtcgc aggcgagccc atcaacgtcg gcttctcgtg
     3601 gggttgaaga gtcttctcgt actcttccca aggcaggtcg tggggacggg tcatgatcgg
     3661 gtccgaagtc cgtccagcaa caacgcgcga atacgctgtc cgaacatatc gcgttcctca
     3721 ggcgtcggtc ggctggcccc tgcgccgagg atcatggcga tggcgcggat gtcgctgggt
     3781 cccagatcgg cgcggacaag accggcggct ttggcgcgtt cgagcgcgga ttcaccggcc
     3841 tcgaccagct tcagacggat gggtttcagg gcgccggagg actgagcctc gcgcagggcg
     3901 gcatcgaggc cggcattgcg gcccaacaca tccgccaact ggtccacgaa ccagaagaaa
     3961 gcctcgggat cgtcgccgcg ttcacgagtt cgagccgcca gatcctcgag ctgctcttcg
     4021 aacaccgcga gcgccaaggc cgtgcggtcg gcgaagttgc gatagagggt cgcgcgcccc
     4081 acccccgccc ggtcggcgat ggcgtccagc ggcacgttgt agccgtgcag agcgaactct
     4141 tcggcggcgg cagccagcag ggcgccacgc cgttcaatcg catcttttct gggggctctg
     4201 gccatggatc gttaagtgga cagtgatgtc cgctttggca ataggccaga atggctagcg
     4261 gtcgaattta tctcgtggtc gtaaaatcat gcacaggttc tggcgtcaga gccgccagat
     4321 gtctgacttc tgaccggttt cgccgagggt ctcggcaacc caactgcaga tttcgcgggc
     4381 agcttcgtcg gtcccggcgg gcggattcac caccaccatc gcgcagccgt tcatcttcat
     4441 cggattggtc agaggcctga gccgaacttc tgcgaccacc gtggggatcg tcgtcccgag
     4501 ccgacgaagg aagccgtcga aggtttccag atccttgagc ggcgtccaga tggcgacggt
     4561 cacgccgggg cgagatttga ccagctccag cgtcgtctcg gcggcgcgca gatagtcgtc
     4621 tggctgttcg aagggcggat cgatcaggat caggtc
//
LOCUS       DASVTM010000252         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_873720,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000252 DASVTM010000000
VERSION     DASVTM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873720"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..170)
                     /locus_tag="VF633_06940"
                     /pseudo
     CDS             complement(1..170)
                     /locus_tag="VF633_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611567.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(219..565)
                     /locus_tag="VF633_06945"
                     /pseudo
     CDS             complement(219..565)
                     /locus_tag="VF633_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612156.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            633..1141
                     /locus_tag="VF633_06950"
                     /pseudo
     CDS             633..1141
                     /locus_tag="VF633_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927305.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1110..>1506)
                     /locus_tag="VF633_06955"
     CDS             complement(1110..>1506)
                     /locus_tag="VF633_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8470831.1"
                     /translation="DRLRSAEPIVAAALRRQWPSAAPRPDATASLAEEADLVLDAAFA
                     GFCADRLSTRERMVTQMVLRGHSNQSVATLLGIAESTVKNHRKSIYAKLAIASQQELF
                     SLFLTHALDRRTHWRPAPTLNVPAANLCP"
BASE COUNT          265 a          495 c          482 g          264 t
ORIGIN      
        1 gatgcctatg gattttggtg gtcagtccac ctcgggaacg ctccaacgcc tgatgctggc
       61 ccctctcttg cgcaggctgc atgctgatgg gcgcgagcga tcgtgctgtc gatcatgaaa
      121 ctctggttgt cgcggtcgtc cgtgagggcg gcgaacacgc gctcccaaat gttgttgcgc
      181 ccgaatgatc ggacaggaca gcgagcacaa caggggtttt ttgggccggc agcaacctcg
      241 agcgcctctt tgaggatctc ggtttccagc gtcttcttcc cgagaagacg atgcaattcg
      301 cggatctgcg cctggagagc acgatactcc aaggtcgcaa cgacctcttc cagcgccctc
      361 gtggcgacca gagcgccctg cgcagccaga cttcgccatc gaaagagctg gttcggcgca
      421 acgccatgac cgacgcgcca ccaggctcac cgtcacgtcg gcctcatagg tctcttggac
      481 aatcgacagc ttcccgggcc gtcgaccacg atcgacgacg ctcgggaccc ggcagaacct
      541 ctgccttcgg ataagaacca gtcatagaca caacaccact cctaatcctt gcgggagtgg
      601 gagatggtgt ccccgtcatt cgggggaaaa tcctgatgga cggacaccgc ccggacgtgt
      661 ggtgttccga tcgctattcg gcccggcagg gccacgggtc cgcgcatcgg acatgccttg
      721 cccatctcgc ccgcgacgtc gcctatgccg aggaggccag cgagggcgcg ctcccctcgc
      781 ggcttcgtct ctggcagaaa cgggccttgc ccgaggcgtc cagcacaagt tccgtcggcc
      841 ccgcgatcag ctctcctggc cttcgcgctt taccccggtc tcgtccagcc gacgaacaat
      901 gcctgcgagc ggtcgctgcg gcctgccgtc atccagcgaa aggtcacgaa cggctatcgt
      961 gccatccggg ccgccagggg cgaagccgac atccgcaccg tcgtcgacac cgcgcctgag
     1021 ccccggcgcc gataccttca agaccaccct ccagaccgtc cggggcctga aattccggaa
     1081 acggggcgtg gataattgcg atgcttgcgt cagggacaaa ggttggcggc agggacattg
     1141 agggtggggg cgggacgcca gtgcgtgcgc cggtccagcg catgggtcag gaaaaggctg
     1201 aagagctcct gctggctggc aatcgcgagt ttggcgtaga tgctcttgcg atggttcttc
     1261 accgtgctct cggcgatgcc gaggagggtg gccaccgatt ggttggagtg gccgcgcaac
     1321 accatttgcg tcaccatgcg ctcgcgcgtc gagagacggt cggcgcaaaa tccggcgaag
     1381 gccgcgtcga gcacgagatc ggcctcctcc gcaagcgacg ccgttgcatc gggtcgtggc
     1441 gccgccgacg gccattggcg acgcagcgcc gcggccacga tcggctcggc gctgcgcaac
     1501 cggtcg
//
LOCUS       DASVTM010000253         6587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_980402,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000253 DASVTM010000000
VERSION     DASVTM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980402"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /gene="ispH"
                     /locus_tag="VF633_06960"
                     /gene_synonym="lytB"
     CDS             complement(<1..539)
                     /gene="ispH"
                     /locus_tag="VF633_06960"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261647.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX8470832.1"
                     /translation="MNAHAPIKRPLNVRLATPRGFCAGVDRAIQIVERAIEKFGAPVY
                     VRHEIVHNRHVVERLKSMGAIFVQELDECPDDRPVVFSAHGVPKSVPANAQARQLLYL
                     DATCPLVSKVHVEAERHYAAGRHILLIGHAGHPEVVGTIGQLPKGAITLVETVEDAQT
                     VQRPLSEDGGEQQLAYATQT"
     gene            726..1421
                     /locus_tag="VF633_06965"
     CDS             726..1421
                     /locus_tag="VF633_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence domain protein"
                     /protein_id="HEX8470833.1"
                     /translation="MRRLLSACSAVVLMAGVLPGVAHAQYNGGQSQSGRQQGQQQGAS
                     GQEEAPSSRRGPRLGSLRQQANAGPCPYVKVLYDAARYVELADNRPAIANVGYAGEIE
                     GVTAGCSYRGEDPITVQMNVLFNLGRGAQATADQRSYRYWIAVTERNTAVLAKEYFDL
                     PVDFKGAQTASVEEERTIVIPRKDTGVSGSNFEILVGFDVTPEMATFNRDGSRFRVNA
                     GSAPAPVAPPPAQ"
     gene            1421..3223
                     /gene="argS"
                     /locus_tag="VF633_06970"
     CDS             1421..3223
                     /gene="argS"
                     /locus_tag="VF633_06970"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX8470834.1"
                     /translation="MTDLKSELGEAVAAAFAAEGVDAALARVTTSDRPDLADFQSNGA
                     LAAAKALKANPRELAARVAERLAADPRLSGVEVAGPGFINFKVSDATLAVRAQAVADD
                     ATLAGASAVATPRKVVIDFAGPNVAKPMHVGHLRSSIIGESLKRLFRLRGDTVWGDAH
                     FGDWGFQMGLLIVAVGDEGKADGFLAEGDGPFPVESPVTLEDLERLYPAAAGTAKEDP
                     AYRDRARKATAELQGGRPGYRALWQHFVTVSRTALQREFGALDVTFDLWNGESDADPR
                     IAGMVEDLKSKNLLVLDDGAQVVHVAGPGETRKKKLADGSVIEAPSPPPLLVISSEGS
                     AMYGTTDLATIVDRRETIDPDLILYVVDERQAEHFEQVYRAAYLAGYAKPGELEHLGF
                     GTMNGTDGKPFKTRAGGVLKLHDLITQATDKARERLREANLGADVDPAEFEDIAHKVA
                     IAALKFADLSNSRTTSYVFDLDRFMSFEGKTGPYLLYQVVRIRSLLRRGAEQGVVVGS
                     IAVVEPAERDLVLTLDAFDQAISDAYDKRMPHLIAEHAYRLAQAFSKFYAACPVLVAP
                     DEPTRGSRLALSKAALDQLVLALDLLGIATPERM"
     gene            3279..4286
                     /locus_tag="VF633_06975"
     CDS             3279..4286
                     /locus_tag="VF633_06975"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX8470835.1"
                     /translation="MSLDAFINGLPKAELHLHIEGSLEPELMFELAKRNKVDIPFDSV
                     EAVRAAYDFSNLQDFLDIYYAGANVLQTEEDFHDLAFAYFQRAAADRVRHAEIFFDPQ
                     THTDRGIPFQVVADGLLSGMKAAETELDVSSQLILCFLRHLDEDAAFATLRKAEPWLD
                     RIRGVGLDSSEVGHPPSKFQHVFAAAGSMGLKRVAHAGEEGPPEYVWEALDLLKIDRM
                     DHGNRSMEDPKLVERLVREGTTLTVCPLSNHKLCVVDDLKDHPVPEMLRQGLKITLNS
                     DDPAYFGGYVNENYRQLAAAVDLSREQLTLLAKNSFEGSFLTSAEKSVYIDEIDAYAA
                     L"
     regulatory      4333..4440
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      4445..4531
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4586..5701
                     /gene="gcvT"
                     /locus_tag="VF633_06980"
     CDS             4586..5701
                     /gene="gcvT"
                     /locus_tag="VF633_06980"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX8470836.1"
                     /translation="MTHESLKTTPLNAAHRALGARMVGFGGYDMPVQYEGVLAEHRWT
                     REHAGLFDVSHMGQCKITGADAISQFERFVPGDYAILKAGKQKYSLLLNAQGGVIDDL
                     MAGKPDHDGLFVVVNAGNKDEDFAFWSANLEGDARLTVLADRSLIAIQGPEAAEVMLK
                     HEPVLAEFGFMDTARLMLFGVDCYISRSGYTGEDGYEISVPNADAERIWNTILEDARV
                     KPIGLGARDSLRLEAGLPLHGHDIDPTTSPVEGALTFALSKSRKEAANFNGADRILKE
                     LADGPSRVRIGLSVKEGAPAREGAEIADLDGNVIGKVTSGGPSPTLGRNIAMGYVAPK
                     YAELGTDLKVIVRGKAAAAEVVAMPFVATRYYRKPKV"
     gene            5713..6078
                     /gene="gcvH"
                     /locus_tag="VF633_06985"
     CDS             5713..6078
                     /gene="gcvH"
                     /locus_tag="VF633_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX8470837.1"
                     /translation="MKFTKDHEWVSLDGDIATVGISKHAADALGDVVFVEVPEVGKTV
                     SKGDSFAVVESVKAASDVYAPVSGDVVEANNTLATAPETVNSAAETDGWFAKIKVSDT
                     SALDALMDQAAYDEYLTTL"
     gene            6125..>6587
                     /locus_tag="VF633_06990"
     CDS             6125..>6587
                     /locus_tag="VF633_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEX8470838.1"
                     /translation="MRYLPLTPDDRTAMLSAIGAKSIDDLFVDVPQAARREGFVDLPR
                     VSGELEVERALSRMAAKNASAGSVPFFCGAGAYKHHVPATVDHVIQRSEFLTSYTPYQ
                     PEIAQGTLQYLYEFQTQVANLTGMAVANASLYDGSTAMAEGVLMATRVTRRN"
BASE COUNT         1175 a         2198 c         2095 g         1119 t
ORIGIN      
        1 gtctgggtgg cgtaggctaa ctgttgctcg cccccatctt cggagagggg ccgctggacc
       61 gtttgcgcgt cctcgacagt ctcgaccaag gtgatcgcgc ccttgggcag ttggccgatc
      121 gtgccgacga cctcggggtg accggcgtga ccgatgagca agatatggcg accggcggcg
      181 taatggcgtt cggcctcaac atggaccttg gacaccaggg ggcaggtggc atcgagatac
      241 agaagctggc gcgcctgcgc attggccggg accgacttcg gcacgccgtg cgccgagaag
      301 acgacagggc ggtcatcagg gcattcgtcc agttcctgaa cgaagatcgc acccatggac
      361 ttcagccgct cgacgacgtg gcggttatga acgatctcgt ggcggacata gaccggggcg
      421 ccgaatttct cgatggcgcg ctcgacgatc tggatggcgc gatcaacgcc ggcgcagaat
      481 ccgcgcggcg tggcgaggcg gacgttcagg gggcgtttga tgggcgcgtg agcgttcatg
      541 accgcaatct agggcgttcg ggacgagacc gccaccgcga cgaggccgca gccatctgat
      601 ttgcccacct tcgttgcgcg catgacgttt gccgcgcctc gctttagccg ctatcagcac
      661 ccagcgtggc agggtcgggc gccgaccctt gatgcgcgcc cattcggctg tcggaaactc
      721 ccttgatgcg tcgccttctc tctgcctgct ccgccgttgt cctgatggcc ggtgtgcttc
      781 ccggtgtggc tcatgcccag tataacggcg ggcaatccca gtccggccgc caacagggcc
      841 agcaacaggg tgcttccggc caggaagagg ctccatcaag tcgtcgcggg ccccgtctcg
      901 gatctctgcg ccagcaggcc aacgcgggcc catgccctta tgtgaaggtt ctctacgacg
      961 cagctcggta cgtcgaactg gcggacaacc gtccggccat cgccaacgtc gggtacgccg
     1021 gcgagatcga gggcgtcaca gcgggctgca gctatcgcgg cgaagatccg atcaccgtgc
     1081 agatgaacgt gctgttcaat ctcggtcgcg gcgcgcaggc gacggcggac cagcgcagct
     1141 atcgttactg gatcgctgtg accgagcgga acaccgccgt gctggccaag gaatatttcg
     1201 acttgccggt cgacttcaag ggcgctcaga ccgcctccgt cgaggaagaa cgcaccatcg
     1261 tcatcccccg caaggacact ggcgttagcg gttcaaactt cgaaatcctc gtcggttttg
     1321 acgtgactcc ggaaatggcg acgttcaacc gtgacggcag ccgattccgc gtcaacgccg
     1381 gttcagctcc cgcgcctgta gccccgcctc cggcacagta atgaccgacc tcaagtccga
     1441 actgggcgaa gcggtcgcag ccgccttcgc cgccgaaggc gtggacgctg cgctggcgcg
     1501 cgtcacgacc tccgataggc ctgatctcgc cgatttccag tccaatggag cgctcgccgc
     1561 cgccaaggcg ctgaaggcca atccgcgcga gctggccgcg cgtgtcgccg aacgtctggc
     1621 tgccgatccg cgcctgtcag gcgtcgaggt ggctggaccg ggcttcatca acttcaaggt
     1681 ttccgacgcg acactcgctg tccgggcgca ggccgtagcc gacgatgcga cgctggctgg
     1741 agcttctgcc gttgcaacgc cgcgcaaggt ggtgatcgac ttcgccggcc ccaacgtggc
     1801 caagcccatg cacgtcggcc atctgcgctc gtcgatcatc ggcgaaagct tgaagcggct
     1861 gttccgcctg cgcggcgaca cggtctgggg cgacgcccac tttggcgact ggggcttcca
     1921 gatgggcctg ctgatcgtcg ccgtgggcga tgagggcaag gccgacggct tcctcgccga
     1981 gggcgacgga ccgttcccgg tggagagccc ggtcacgctg gaagacctcg aacgcctcta
     2041 tcccgctgcc gccggcaccg ccaaggaaga tcccgcctat cgtgaccgcg cccgcaaggc
     2101 gacagcggag ttgcagggcg gacgccccgg ctaccgcgcg ctgtggcagc atttcgtcac
     2161 cgtcagccgc acggcgctgc aacgcgagtt cggcgcgctg gatgtcacct tcgacctctg
     2221 gaacggcgag agcgatgccg atccgcgcat cgcgggcatg gttgaggacc tcaagtccaa
     2281 gaacctgctg gtcctcgatg acggcgctca ggtggtccac gtcgccggcc ccggcgagac
     2341 gcgcaagaag aagctcgccg acggttcggt gatcgaggcc ccgagcccgc cgccgctgct
     2401 ggtcatcagc tccgaaggct cggccatgta cggcacgacc gatctggcga ccatcgtcga
     2461 ccgccgcgag accatcgatc ccgacctgat cctctatgtc gtcgacgagc gtcaggccga
     2521 gcatttcgaa caggtctacc gcgccgccta tctggccggt tacgcgaagc cgggcgagct
     2581 cgaacacctc ggtttcggca ccatgaacgg caccgacggc aaaccgttca agacccgtgc
     2641 gggcggggtg ctgaagctgc acgacctgat cacccaggcc accgacaagg cgcgcgagcg
     2701 tctgcgtgag gccaatctgg gcgctgacgt cgatccggcc gagttcgagg acatcgccca
     2761 caaggtcgcc atcgcggccc tgaaattcgc cgacctgtcg aacagccgca ccacaagcta
     2821 cgtcttcgat ctcgaccgct tcatgagctt cgagggcaag accgggccgt acctgctgta
     2881 tcaggtcgtc cgcatccgct cgctgctgcg gcgcggggcg gagcagggcg tcgtcgttgg
     2941 ctctatcgcg gtcgtcgagc ctgccgagcg cgatctggtg ctgacgctgg acgccttcga
     3001 tcaggccatt tccgacgcct acgacaagcg catgccgcac ctgatcgccg aacatgccta
     3061 ccgtctggcg caggcgttct cgaaattcta tgcagcctgt ccggttctgg tggcgcccga
     3121 cgagcccacg cgcggttcgc gtctggcgct gtcgaaagct gcgctggatc agctggtgct
     3181 ggcgttggac ctgctgggca tcgcaacgcc ggaacggatg tagcgaaccg gctccggttt
     3241 caggttagac tgtgtcgatc cccgatccgg agcctgccat gtccctcgac gccttcatca
     3301 acggcctgcc caaggccgag ctgcacctgc atatcgaggg cagtcttgag cccgagctga
     3361 tgtttgagct ggcgaagcgg aacaaggtcg acatcccgtt cgacagcgtc gaggccgtgc
     3421 gtgcggccta tgatttctcg aacctgcagg acttcctcga catctattat gcgggcgcca
     3481 acgtcctgca gaccgaggaa gacttccacg atctcgcctt cgcctatttc caacgagcgg
     3541 ctgccgaccg ggtgcgtcac gcggaaatct tcttcgaccc acagacccac accgaccggg
     3601 gcatcccgtt tcaggtcgtc gccgatgggc tgctgtcggg catgaaggcg gccgagaccg
     3661 agctcgatgt ctcttcgcag ttgattctgt gcttcctgcg ccatctcgat gaagacgcgg
     3721 ccttcgcgac tttgcggaag gctgaacctt ggctggaccg gatcaggggc gtcggtctgg
     3781 actcctccga ggttggccac ccgccatcga aattccagca cgtgttcgcc gccgccggat
     3841 cgatgggctt gaagcgtgtc gcccacgccg gtgaagaagg cccgccggag tatgtctggg
     3901 aggcgctcga cctcctgaag atcgaccgga tggaccacgg caatcgttcg atggaagacc
     3961 cgaagctggt cgagcggctg gtgcgcgagg gcacgacgct gacggtctgt ccgctgtcga
     4021 accacaagct ttgcgtcgtc gacgatctca aggatcaccc ggtgccggag atgctgcgcc
     4081 aagggctgaa aatcacgctg aactctgacg atccggccta tttcggcggt tacgtgaacg
     4141 agaactaccg tcagctggcg gctgcggtgg acctgagccg cgagcaactg accttgttgg
     4201 cgaagaacag cttcgaaggt tcgttcctga cctcggccga gaagtcggtc tatatcgacg
     4261 agatcgacgc ctacgcggca ttgtgatcgt cggtcgcgct tgacttgaga cgggagtcga
     4321 ggcctaggtc gcctcgctct cgcgggagag aacggggacc tccattctat tttggaggcg
     4381 accgacgccg aaggcgcaac cgccccggaa acgctcaggc aaacggaccg cgagaacatt
     4441 cggacgctgg aaagtcactc cttgcgagtg cgccgacgga gcaaggcggc cttcttcagg
     4501 tcgtcggaat ctctcaggct tcaggacagc gggggcgcga agacggcgga gcttccgcct
     4561 caagacgcga ctctgaaagc cgaacatgac ccacgaaagt ctcaagacaa cacccctgaa
     4621 cgccgcgcac cgcgcgctgg gcgcccgcat ggtcggcttc ggcggctacg acatgccggt
     4681 gcagtacgaa ggcgtgctgg ccgagcaccg ctggacgcgt gaacacgcag gcctgttcga
     4741 cgtgtcgcac atgggccagt gcaagatcac cggcgcggac gccatttcgc agttcgagcg
     4801 cttcgtgccg ggcgactatg cgatcctgaa ggcaggcaag cagaaatact cgctgctgct
     4861 gaatgcccaa ggcggcgtca tcgacgacct catggccgga aagcctgacc atgacggcct
     4921 gttcgtggtg gtcaacgccg gcaacaagga cgaggatttc gcgttctgga gcgccaatct
     4981 cgagggcgac gccaggctga cggtcctcgc agaccggtcg ctgatcgcca tccagggccc
     5041 ggaggccgca gaggtgatgc taaagcatga gcccgtgctg gccgagttcg gcttcatgga
     5101 cacagcccgc ctgatgctgt tcggcgtcga ctgctacatc tcgcgctcgg gctacaccgg
     5161 cgaggacggc tacgagattt ccgtgccgaa cgccgacgcc gaacgcatct ggaacaccat
     5221 cctcgaggac gcccgcgtca agcccatcgg tctcggcgct cgcgacagcc tgcgcctcga
     5281 ggccggcctg cccctgcacg gccacgacat cgatcccacg acctcgccgg tcgagggcgc
     5341 gctgaccttc gccctgtcca agtcacgcaa ggaagccgcg aacttcaacg gcgccgaccg
     5401 catcctgaag gaactggccg acggtccgag ccgtgtccgc atcggcctga gcgtcaagga
     5461 aggcgccccg gctcgtgaag gcgccgagat cgccgatctt gacggcaacg tcatcggcaa
     5521 ggtcacctcc ggcggcccgt cgccgaccct cggccgcaac atcgccatgg gctatgtcgc
     5581 gccgaaatac gccgagctcg gcacggacct gaaggtcatc gtgcgcggca aggccgccgc
     5641 cgccgaggtc gtcgccatgc ccttcgtcgc cacccgctat tatcgcaaac ccaaagtctg
     5701 agagccccag ccatgaagtt caccaaggat cacgagtggg tcagcctcga cggcgacatc
     5761 gccaccgtcg gcatctccaa acacgctgct gacgcgctcg gcgacgtcgt gttcgtcgaa
     5821 gtgcccgaag tcggcaagac ggtctccaag ggcgacagct tcgccgtggt cgagagcgtc
     5881 aaggcggcgt ctgacgtcta cgccccagtc tcgggcgacg tggtcgaagc caacaacacg
     5941 ctcgccacgg cgccggagac cgtcaactcg gccgccgaaa ccgacggctg gttcgcgaag
     6001 atcaaggtct ccgacacctc ggcgctcgac gccctgatgg atcaggccgc ctacgacgaa
     6061 tacctgacca cgctctaagc gacccgccac gtctgctcct ttccacgcac gggacctcct
     6121 caccatgcgc tacctgcccc taacccccga cgaccgcacg gcgatgctgt ccgccatcgg
     6181 cgccaaatcc atcgacgacc tgttcgtgga cgtgccgcaa gcggcgcgtc gcgagggctt
     6241 cgttgatctg cctcgcgttt ccggtgagct ggaagtcgag cgggctctgt ctcgcatggc
     6301 cgccaagaac gcctcggccg gttccgtgcc gttcttctgc ggggcagggg cctacaagca
     6361 ccacgtcccg gcgacggtgg atcacgtgat ccagcgttcg gagttcctga ccagctacac
     6421 gccttaccag ccggaaatcg cgcagggcac gcttcaatac ctgtacgaat ttcagaccca
     6481 ggtcgcgaac ctgacgggca tggcggtcgc caacgcctcg ctctatgacg gctcgaccgc
     6541 catggccgaa ggtgtgctga tggccacgcg ggtcacgcgc cggaaca
//
LOCUS       DASVTM010000254         4265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_24230, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000254 DASVTM010000000
VERSION     DASVTM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24230"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..2928
                     /locus_tag="VF633_06995"
     CDS             175..2928
                     /locus_tag="VF633_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470839.1"
                     /translation="MTGWLGLVFSALFLAAVWLIWRASDRSRPMTSLVVVLGVLIAPA
                     LVWTSLIDASLNGRFGDMHLSLTRLTVDVTRGALSIGGGPDDDLFIRGASPAVVTVPR
                     QTPGAGPVLMEVSTKPASETLSVAAVRHGNVYDYIGAQDLSPGMAVCLTGCEAIDAVW
                     YRFERAPWRFERLDGAAGPTLPQRRAFGLAPTVSWKPSQAIHPLDRVLPGAPRTPGFV
                     YQTGKLFGGWKILLPDPGARLGKVEGGRVTPLAAPAPGPVPVDTARALSIWDVRTYNA
                     ADPGDPLGRLQERREIALSQTGTVLTATLSTPAVERVGRCPRNGSPSPVAPDFPVLGG
                     DLSNALTQDLPYPEPGHCAELASGTFRLTDPVALGKSAELRLDRLGGPWLLGWLSLAW
                     AVVVLAVWGDDWRSDAASGGRRLQWMLFVALQALLAMRMLIAFAGLAADPELAGGLSV
                     VGEAGLAYLAMTGLLLLLGPPTERRALRMALVFGFVVAVTLALHLWMTAVPGPIFLAS
                     FKPAFWTPTPGGLALVATVAVLVVSGLHALAQSEGQTTLIHTIRSRIEAIVSARFVGP
                     VSDWRTVLPVTGFIRLLLGLASIKERLPVLGFAVSALYTPALILGFSGLMADGARSSG
                     KRRLALGLVFCASLAILFVALPVGVKDNGYAIVGVAVLVMAAWGVWERRRQATTGPER
                     LAALAWAAPLIGAVLVILIASLLFQAPAAASRDDIARAAVAASDQPALDILARAGAYS
                     QNQLRLWAFGSPDQVERYGSWEAENLRVWSQHLSDYTATLFGHGYLAPVNLSVLKPVQ
                     LNDNVSTIHVMAPYGRFGAAALLLCLAVLAAAAARVTRSEGDGPPSQGRLIGLMSLWI
                     LFGVGAYVVLANLQLAPFTGRNVYLLAAASDSDLLEGMTLFVMAWLGLTWVPRTRLED
                     EIV"
     gene            2928..>4265
                     /locus_tag="VF633_07000"
     CDS             2928..>4265
                     /locus_tag="VF633_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470840.1"
                     /translation="MARGKSIIAAGKKKAAAQAALKPSAPPPGDPVDAPPPLSPILPR
                     ATFRIAPGWFVGLAFVLLIVGAPWIRGLLGHPPSDIHAVRQADAYNALINDLVLKRGW
                     LEITQRPGGDYRVQASTLAPKEAEGWQAFVRSSYLRVDMTEPGRWLFDDDLGRGVRLT
                     EIDPTAHQVLGPYAGTLQWRGDIQFRTGSSGGASLRRTGEPQTFLFRPARGASEAGLT
                     TATIPLFKAPETGAQVWPRRYRFVDEIEVASVIALDNQNALVRVIGGSPVTVTVAGVE
                     ASPGSGEIEYLRLPADRDLVFTKGEHADRFVYEVAAPTVSRYEAVSGQRLRVAGLESF
                     ARALEPAVTRRDDRPIVVTLDQRLQAEAESQLHAVADQLPASPAFRSAVTMMDAQTGE
                     VLAVASLPVPAADQTDTRGRHRFTTTNHNFDLMPVGSVAKAPLAMAILQTFPAL"
BASE COUNT          536 a         1461 c         1472 g          796 t
ORIGIN      
        1 tgatcaagcg cggctggctc cgtcggtcgg agcaggcgcg ggcgcggtgc tcgccggatt
       61 gatcctgctg ttgctgggcg ccgggatgat cgtcggcgtc tatgccttca cccgcgtccg
      121 cgacgcctac cgtcgcctct acgtctcgca ccatcggcct gacccggaga gccgatgacc
      181 ggctggctgg ggctcgtctt ctcagcgctg ttcctggccg ccgtctggtt gatctggcga
      241 gcgtccgacc ggtcacgacc gatgacgtcg ctggtcgtcg tgctcggcgt gctgatcgcg
      301 cccgccttgg tctggacgag cctgatcgat gcgtcgctga acgggcggtt cggcgatatg
      361 cacctgtccc tgacgcggct gaccgtggac gtgacgcggg gcgccctgag catcggcgga
      421 ggcccggacg acgacctgtt cattcgcggc gcttcgcctg ccgtagtgac ggttccgcgc
      481 cagacgccgg gcgccggtcc ggtcctgatg gaagtctcga ccaagcccgc ctccgagacg
      541 ctgtcggtcg cggcggtgag gcacggcaac gtctacgact acatcggcgc tcaggattta
      601 agcccgggca tggcggtctg cctgaccggc tgcgaggcca tcgacgccgt ctggtaccgg
      661 ttcgagcgtg cgccgtggcg gttcgagcgt ctggacggag cggcgggccc gaccctgcca
      721 cagcgccgcg cattcggcct tgcacccacg gtctcgtgga agccatcgca agccatccat
      781 ccgctggatc gcgtcctgcc cggagcgccc cggacccctg gcttcgtcta tcagacgggc
      841 aagctctttg gcggctggaa gatattgctg cctgatcccg gcgctcgcct cggcaaggtc
      901 gagggcgggc gggtcacgcc cttggccgcc ccggcgccgg ggcccgttcc ggtcgatacg
      961 gcgcgcgccc tgtcgatctg ggacgtccgc acctacaatg ccgccgatcc gggtgatccg
     1021 ctggggcggc tgcaggaacg gcgcgagatt gcgctgagcc agaccggaac ggtcctgacc
     1081 gccaccctgt cgaccccggc ggtggagcgg gtggggcgct gccctcgcaa cggatcgcca
     1141 tcgccggtcg cgcccgattt tccagtgctt ggcggcgacc tgtccaacgc cctgacgcag
     1201 gatctgccct atccggagcc cggccactgc gctgagctgg cgtcggggac gttccgcctg
     1261 accgatcccg tcgcgctcgg gaagagcgcc gagctgcggc tcgaccggct gggcggacct
     1321 tggctgctcg gctggctgag tctggcatgg gcagtggtag tcttggcggt ctggggcgac
     1381 gactggcggt cggatgcagc gtccggcggt cggcggttgc aatggatgct gttcgtggcg
     1441 ctgcaggcct tgctggcgat gcggatgttg atcgctttcg ccggtctggc cgccgatccc
     1501 gaactggcgg gaggcctgtc ggtcgtgggc gaggcgggcc tggcctacct cgccatgact
     1561 ggtctgttgc tgctgctcgg tccgccgacc gaacgccgtg cgctacggat ggcgctggtg
     1621 tttggcttcg tggtcgccgt cactctcgct ttgcacctgt ggatgactgc cgtgcccggc
     1681 ccgatctttc tggcgtcgtt caagcccgcc ttctggacgc cgacgccggg aggtcttgca
     1741 ctggtcgcca cggtggcggt gcttgtcgtc agcggccttc acgctttggc tcagagcgag
     1801 ggccaaacga ccttgatcca tacgatccgc tctcgtatag aagctattgt ttctgcgagg
     1861 ttcgttggtc ccgtctcgga ttggcgcacg gtgcttccag tcaccggatt catccggctt
     1921 ctgctcggtc tggcgtcgat caaggagcgg ttgccggtac tcggtttcgc cgtcagcgcc
     1981 ctctacacgc ccgcattgat cctcggtttt tcgggcctga tggcggatgg agcgaggtcg
     2041 tcgggcaagc gccgcttggc gctcggcctc gtcttctgtg catcgctcgc catcctgttc
     2101 gtcgccttgc ccgtcggtgt gaaggacaac ggctatgcca ttgtcggtgt tgcggttttg
     2161 gtcatggcgg catggggcgt gtgggagcgg cggcgacagg cgaccaccgg ccccgaacgc
     2221 ctcgccgcgc tggcctgggc cgcaccgctg atcggcgccg ttctggtgat cctgatcgcc
     2281 tcgctgctgt tccaggcccc cgccgctgcg tcccgcgacg acatcgcccg cgccgccgtc
     2341 gccgcgtcgg accagccggc gctggatatt ctggcgcggg ccggcgccta cagccagaat
     2401 cagctccgtc tgtgggcctt cgggtcgccc gatcaggtcg aacgctatgg gtcgtgggag
     2461 gcggaaaacc tgcgcgtctg gtcccagcac ctgtccgact ataccgcgac cctgttcggc
     2521 cacggctatc tggcgcccgt caatctgagc gtgctgaagc cggtgcagtt gaacgacaac
     2581 gtctcgacca tccacgtcat ggccccctac ggtcggttcg gcgcggcggc gctgttgctg
     2641 tgcctcgcgg tgctggcggc ggcggcggcg cgggtgaccc ggtctgaggg cgatgggccg
     2701 ccgagccagg gtcggctcat cggcctgatg tctctgtgga tcttgttcgg cgtcggcgcc
     2761 tatgtggtgc tggccaatct gcaactcgcg cccttcacgg ggcgcaacgt ctatctgctg
     2821 gccgccgcct ccgacagcga tctgctggag gggatgacgc tgttcgtcat ggcgtggctc
     2881 ggcctgacgt gggtcccgcg cacccggctc gaagacgaga tcgtctgatg gcccgcggga
     2941 agtcgatcat cgccgcgggt aagaagaagg ccgctgcgca agcggccttg aagccctctg
     3001 cgccgcctcc cggtgatccc gtcgatgcgc cgccgcccct ttccccgatc ctcccgcgcg
     3061 cgacattccg catcgccccc ggctggttcg tcggtctggc ctttgtcctg ctgatcgttg
     3121 gcgcgccgtg gatacgggga ctgctcggcc atccgccgtc cgacatccac gcggtgcgtc
     3181 aggccgacgc ctacaatgct ttgatcaacg acctcgtgct caagcgcggc tggctggaga
     3241 tcacgcagcg accgggaggc gactatcgcg tgcaggcgtc tacgctcgcg ccgaaggaag
     3301 ccgaaggctg gcaggctttc gtgcgcagtt cctatctgcg cgtcgacatg acggagccgg
     3361 gccgctggct gttcgacgat gacctcgggc gcggcgtgcg gctgaccgag atcgatccga
     3421 cggcgcatca agtgctcggg ccctatgccg ggacgctgca atggcgcggc gacatccagt
     3481 tccgcaccgg ctcctcgggc ggcgcgtcgc tcaggcggac cggcgagccg cagaccttcc
     3541 tgttccggcc cgcgcgcggc gcctcggagg ccgggctgac gaccgccact atccccttgt
     3601 tcaaggcccc cgaaacgggc gctcaggtct ggccccggcg gtatcgcttc gtcgatgaga
     3661 tcgaggtcgc cagcgtcatc gcgctcgaca accagaacgc ccttgtccgg gtgatcggcg
     3721 gctcgcccgt gaccgtcaca gtcgccggag tggaggcctc cccgggctcg ggagagatcg
     3781 agtacctgcg gctgcccgcc gaccgcgatc tggtcttcac caagggcgag cacgcggacc
     3841 ggttcgtcta tgaagtcgcg gccccgaccg tctcccgcta cgaggccgtc agcggtcagc
     3901 gtctgcgcgt cgccggtctg gagagtttcg cgcgggcgtt ggagcccgcc gtcacccgcc
     3961 gcgatgacag gcccatcgtc gtcaccctcg atcagcggct gcaggccgag gctgagagcc
     4021 agttgcacgc ggtggccgac caattgcccg ccagcccggc cttccgatcg gccgtgacga
     4081 tgatggatgc gcagacgggc gaggtcctcg ccgtcgccag cttgccggtt ccggcggctg
     4141 accagaccga cacgcgcggt cgccatcgct tcaccacgac caaccacaat ttcgatctga
     4201 tgccggtggg atcggtggcc aaggcgccgc tggccatggc gatcctgcag accttcccgg
     4261 ccttg
//
LOCUS       DASVTM010000255         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_24304, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000255 DASVTM010000000
VERSION     DASVTM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24304"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VF633_07005"
     CDS             <1..1124
                     /locus_tag="VF633_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HEX8470841.1"
                     /translation="QLPQETTMRQKPIALLSAVALLTLGACATHEVDFQGEGAGVIGD
                     GTTVHATLETPSVGAPGQDAADDPAVWASVTPVLVQGHETPGFVAGTDKKAGLYIYGL
                     DGTILQFLPEGLLNNVDIVDGLMVEGRRQLVLGASDRTPGKVGITLYLFDPAGTGANA
                     VRLWGHVATDVVEPYGFCFAKRGDAIDAILVGHEGEVRQFALTASADGKPSARETRRF
                     EIGSISEGCAADPQTNALYLTEENVGLWRYGLDAASGATRTFIQPIAAGVLVADAEGL
                     TTLTDGEKRYLIASSQGDSTFPVWRIDGPAPVYVGRIVVRDAVVDGVTGTDGVAALGG
                     RVGDRFPEGLVVVQDDINEGGTQNFKYIDWRDIRAALGL"
     gene            complement(1223..1876)
                     /locus_tag="VF633_07010"
     CDS             complement(1223..1876)
                     /locus_tag="VF633_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470842.1"
                     /translation="MATVTRLYDSHTQALDAVSDLEAAGFNADDISIVSNNTDNWHAG
                     HQHSDKGGSGPLGDRNGDGENDVADGAGKGATTGGLVGAGAGLLAGLGMLAIPGLGPV
                     VAAGWLASTAVGAAVGAAAGGATGGLLGALKEAGHSDEDANVYAEGVRRGGTLVSVKV
                     SNGDESRAESILHGRQGFDAASRGEAYRAGGWSQFDSNAQPYTSEEISRERSLYRQV"
BASE COUNT          326 a          683 c          702 g          386 t
ORIGIN      
        1 accagcttcc tcaggagacc accatgcgcc agaaaccgat cgccctgctg tcagccgtcg
       61 ccctgctgac actcggcgcc tgcgccacgc acgaagtcga cttccagggc gagggtgcag
      121 gtgtgatcgg cgacggaacg actgttcatg cgaccctgga aacgccctcg gtcggcgctc
      181 ccggtcagga cgccgccgat gatccggctg tatgggcctc ggttacgccg gttctggtgc
      241 aaggccatga aacgccggga ttcgttgctg gcacggacaa gaaggccggg ctttatatct
      301 acgggctgga cgggacgatc ttgcagttcc tgccagaggg cctgctcaac aatgtcgata
      361 tcgtcgacgg cctgatggtc gagggacgcc gacaactggt tctcggcgcc agcgatcgaa
      421 ccccgggcaa ggttggcatc acgctatatc tgttcgatcc agcggggacc ggagctaacg
      481 ccgtccggct ctggggccac gtcgcgactg acgtcgtgga gccctacggt ttctgtttcg
      541 ccaagcgcgg cgacgcgatc gacgccatcc ttgtgggcca cgaaggcgag gtgcgacaat
      601 tcgccttgac cgcctccgcc gatggaaaac cgagcgcccg cgagacgcgc cggttcgaga
      661 tcggctcgat ctcggaaggc tgcgccgccg acccgcagac caacgccctc tacctgactg
      721 aggagaatgt cggtctgtgg cgctatggcc tcgacgcggc gtcgggtgcg acccgcacct
      781 ttatccagcc catcgcggcc ggcgtgctgg tcgccgacgc cgaaggtctg acgaccctca
      841 ccgacggcga gaagcgctat ctgatcgcct ccagccaagg cgattcgacc ttccccgtct
      901 ggcgcatcga cggcccggcg ccagtgtatg tcgggcggat cgtggtgcgg gacgctgtcg
      961 tagacggcgt gaccggcaca gacggggtcg cagccctcgg cggccgtgtc ggcgatcgct
     1021 ttcccgaggg tctggtggtc gtgcaggacg acatcaacga gggcggcacc cagaacttca
     1081 aatacatcga ctggcgcgac atccgcgctg cgctcggcct ctgaccctct ggccggagac
     1141 ggtctggcgt gggcgatctg cggagcagac atcgccatct gaaatggccc gtcgcagacg
     1201 cgacgggcca aatctgcttc agctagacct gacggtagag gctgcgttca cgcgagattt
     1261 cctcgctggt gtagggttgt gcgttgctgt cgaactggct ccatcctcca gcgcgatagg
     1321 cttcaccccg cgaggcggca tcgaagccct ggcgaccgtg caggatgctt tccgcgcgac
     1381 tttcgtcccc gtttgaaacc ttgacgctga cgagcgtgcc gccgcgtcgc acgccttcgg
     1441 cgtaaacgtt ggcgtcttcg tcggagtgac ctgcctcctt cagcgcgccg aggagtccgc
     1501 ccgtcgcgcc gccggccgct gcgccgacag cggcgccgac ggctgtcgaa gccagccatc
     1561 ctgccgccac cacaggccca aggccgggga tggcgagcat gccgagacct gcaagcaagc
     1621 cggcccctgc gccgaccaga ccgccggtgg tcgcgccctt gccggcgccg tcggcgacgt
     1681 cgttctcgcc gtcgccgtta cggtcgccca gaggccccga accgcctttg tcggaatgtt
     1741 gatggcctgc gtgccagttg tcggtgttgt tcgagacaat gctgatgtcg tctgcgttga
     1801 agccggcggc ttcgaggtca gagacggcgt cgagggcctg ggtgtggctg tcatagagac
     1861 gtgtgacggt ggccatgagt ggctgtcctt ccgatttgaa tgtgggggag tcttggcgct
     1921 cgcgcttact gcgcggtgac gacgcccttg tagtcgaccg agacggtttt ctgggctccg
     1981 tcatgggtcg cggatgccga ccagatgccg ttgtcgctct gggtcagcgc gccgatggcg
     2041 gtgtaaccct gcttctccat ggcttcgcgc gcctgggcct ccgtgaagga gttggcg
//
LOCUS       DASVTM010000256         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_505290,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000256 DASVTM010000000
VERSION     DASVTM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505290"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..876
                     /locus_tag="VF633_07015"
     CDS             148..876
                     /locus_tag="VF633_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX8470843.1"
                     /translation="MITTDPDTEPVDRASLVKGYEVEKGEYILLTEDEIRSVKLESTR
                     TIEIDRFVSEDEIDRIYWGNPYYLAPEGKLAQEAFGVIREAMKRSGQIALGRVVLSTR
                     ERLLALEPRGLGILAYSLRTESEVRAEDDIFRGISAEPPDEAMISIAERIIQQQHGPF
                     EPSRFVDRYEEALRALIATKQRGGKPVQTVASDDANVTDLMAALRASLQGAGVKRSVA
                     KPRTPGRAATVTPITRAPARRRAG"
     gene            930..1070
                     /locus_tag="VF633_07020"
     CDS             930..1070
                     /locus_tag="VF633_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470844.1"
                     /translation="MLTLVGGGRLADVGLLRKAAFIIASDIALVAGRGFDELAFGQDG
                     LL"
     gene            1070..1447
                     /locus_tag="VF633_07025"
     CDS             1070..1447
                     /locus_tag="VF633_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470845.1"
                     /translation="MPKGLCDRSPGSASSPASPPVTPDGRYIVVRGRLWRRSNPDLTP
                     DRRTALVHDLMAARRAVRDASARQETDAVRVARERVDSAKTGLGERGPVWWTDGAPDL
                     NRHMARTTPYAAWFASLPEDSAN"
     gene            complement(1402..1623)
                     /locus_tag="VF633_07030"
     CDS             complement(1402..1623)
                     /locus_tag="VF633_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470846.1"
                     /translation="MARAIDHPNETGKWTLLERSGHIVLWRRERSDPFPVTQFYLIAP
                     PRQTEVLYDETRARAQFALSSGKDANQAA"
     gene            1760..2050
                     /locus_tag="VF633_07035"
     CDS             1760..2050
                     /locus_tag="VF633_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470847.1"
                     /translation="MPEPAIRRIIHGDMDAFYASVEQRDDPTLRGRPLAVGQGARRGV
                     VAAASYNARAFGVRSAMPSTIALRKCPELIFAPPRFEAWIQPARAWRPEEAK"
     gene            complement(2147..2914)
                     /locus_tag="VF633_07040"
     CDS             complement(2147..2914)
                     /locus_tag="VF633_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8470848.1"
                     /translation="MMFSSSRSPLRRAGPAPVIARFNALAKLNANEANVLARLDDWRW
                     DPPGSELVRDRTAWLPRVLVSGWAARVRWLDDGRRQIIGLILPGDGIGLCRRPQPLDL
                     CPLLALTPVQTLDASPIMRAVQDETDYARLSHAVNIAAALDEAWLMEQVVRLGRLTAY
                     ERVAHLILEVRARLRVLGPEVDGKIPFPLTQEILADVTGLSLMHVNRTLQQLRREKLI
                     ELGQGRLKIIDVQALMDLSGFREPRPDAWIFEKSCWI"
     gene            complement(2911..3273)
                     /locus_tag="VF633_07045"
     CDS             complement(2911..3273)
                     /locus_tag="VF633_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470849.1"
                     /translation="MTDAEAAGFDHLACPTAADLAGPSTPDLGVSTVVVSETTGRVYN
                     QRPLVPIPLDAWEEPSLILDWNERCVLMREAPAQTGFGLELIGRGRPGELDVGVEVAF
                     SPPGFRCHIELPLPRRGG"
BASE COUNT          583 a         1090 c         1134 g          556 t
ORIGIN      
        1 gtatcgaccc acatggtcgg gacacctgaa gctttcgctc gtgacctgcc cggtttcgct
       61 tttcactgcg acctcaagcg ccggcgacgt gaagttcaac ctgatcaatc cggagaccca
      121 caaccggatc aagatgatca ccaccgaatg atcaccaccg acccggatac cgagccggtc
      181 gaccgcgcca gcctggtcaa gggctatgag gtcgaaaagg gcgaatacat tctgctgacc
      241 gaagacgaga tccggtcggt gaagctcgaa agcaccagga cgatcgagat cgaccgcttc
      301 gtgtccgagg atgagatcga ccgcatctac tggggcaatc cctattatct cgcgccggaa
      361 ggcaagctcg cccaggaggc cttcggcgtc atccgtgagg ccatgaaacg ctccggccag
      421 atcgcgcttg gtcgggttgt tctgtccacg cgcgagcgcc tgctggctct ggagcccagg
      481 gggctgggca ttctcgccta cagcctccgg accgagagcg aggtccgggc cgaggacgac
      541 atcttccggg gcatcagcgc cgagccgccg gacgaggcga tgatctccat cgccgagcgc
      601 atcatccagc aacagcatgg gccgttcgaa ccgagccggt tcgtcgaccg ctatgaagag
      661 gcgctcaggg cgttgatcgc caccaaacag cgcggtggaa agccggtcca gaccgtcgcg
      721 tccgacgacg ccaatgtcac cgacctgatg gccgccctac gggccagcct gcagggggcc
      781 ggggtgaagc ggtccgtcgc caagccgcgg acgcccggcc gggccgcgac ggtgacgccc
      841 atcacccggg cgccggcccg gcgacgcgcc ggctagtgcg gtcgacgatc gccccggtcg
      901 ccctcgccgg gcctggcggg cgtcgtcgaa tgctgacgct ggtcggcggc ggacgacttg
      961 ccgacgtcgg tctgttgcga aaagcggcct ttattatcgc gtcggacata gcgcttgtcg
     1021 ccgggcgtgg gttcgatgag ctcgcgtttg gacaggacgg cctcctgtga tgccgaaggg
     1081 actctgcgac aggtctcccg gttccgcgtc gagccccgcc tcaccaccgg tcacgccgga
     1141 cggtcgctat attgtcgtgc gcggtcgcct ctggcggcgc tccaacccgg atctgacgcc
     1201 ggaccgtcgc acggcgctgg ttcacgatct gatggcggcc cgtcgcgcgg tgcgcgacgc
     1261 ctcagcccga caggagaccg atgccgtgcg tgtcgcacga gagcgggtcg attccgccaa
     1321 gacgggcctc ggcgagcggg gaccggtctg gtggacggac ggagccccgg atctcaatcg
     1381 tcacatggcg cggacgacgc cctacgccgc ctggttcgcg tccttgcccg aagacagcgc
     1441 gaactgagcc cgggcgcggg tctcgtcata aagcacttcg gtctgccgtg gcggtgcgat
     1501 gagatagaac tgggtgacgg gaaagggatc ggagcgctcg cgccgccaca ggacgatatg
     1561 gccgctccgc tcgagcagcg tccattttcc ggtttcgttg gggtgatcga tcgctctggc
     1621 catgaccgac gataatcccc tgggaggcga atgcggaagg gtcgcctcgc cggttctcgc
     1681 ggatggagcc tccagtgtgg cggcagacac acggcgggcg tgttcctttt gtgttccggt
     1741 ttcgccaggg tggagttcca tgcctgaacc cgcgatccgc aggatcatcc acggcgacat
     1801 ggacgccttc tatgcgtcgg tggaacagcg cgacgatccg acgctgcggg gccgccccct
     1861 tgccgtcggc cagggcgcgc ggcgcggcgt ggtggcggcc gcgagctaca acgcccgcgc
     1921 cttcggcgtg cgctcggcca tgccctcgac catcgccctg cgcaaatgtc ccgagttgat
     1981 tttcgcgccg ccgcggttcg aggcctggat ccagccggca agggcctggc ggcctgagga
     2041 agctaagtga caatgctatg gagccagcac cggattttga accggcccga gaagcgtccg
     2101 ccggccattc cttcacgcat gtttgtggat cgagcggcat tcttgcttaa atccagcagc
     2161 ttttttcaaa tatccatgcg tcgggcctcg gttcgcggaa acccgagagg tccatcaggg
     2221 cttggacatc gataattttg aggcggccct gaccgagctc gatcagtttc tcacgccgga
     2281 gctgctgaag ggtcctgtta acgtgcatca ggctcagacc cgtcacgtcg gcgagaattt
     2341 cctgcgtcag cggaaacggg atcttaccgt cgacttccgg cccgagcacc ctcaagcggg
     2401 cgcggacttc gaggatgaga tgggccaccc gttcatacgc ggtcagccgg ccgagtcgta
     2461 cgacctgctc cattaaccag gcttcgtcca gtgcggccgc gatgttcact gcgtgagaaa
     2521 ggcgggcgta atccgtctcg tcctggaccg cgcgcatgat cggcgaggcg tccagcgtct
     2581 ggaccggcgt aagggcaagg agcggacaga ggtccagggg ctggggccgg cggcacagac
     2641 cgatcccgtc cccgggaagg atgagtccga tgatttggcg tcgtccgtcg tctagccacc
     2701 gcacacgcgc ggcccagccg gacaccaaaa cgcgcggaag ccaggcggtc cggtcccgga
     2761 ccagctcgga gcccggcggg tcccaacgcc agtcgtcgag cctggcgagc acgttcgcct
     2821 cgttggcgtt gagtttcgca agcgcattga aacgggcgat aacgggagcg ggccctgcac
     2881 ggcggagcgg gctgcgtgat gagctgaaca tcatccgccc ctgcggggca gggggagttc
     2941 gatgtggcag cgaaaccccg ggggcgagaa ggcgacttcc acacccacgt cgagttcccc
     3001 gggccgtccg cgcccgatga gctccaggcc gaatccggtc tgggccgggg cttcgcgcat
     3061 gaggacgcat ctctcgttcc agtcgaggat cagcgagggc tcttcccagg cgtccagcgg
     3121 gatcggaaca agaggccgct ggttgtagac ccttcccgtc gtttccgaca cgacgaccgt
     3181 agaaacgccg aggtcgggcg tcgacggacc cgccagatcc gccgccgtgg ggcaggcaag
     3241 atgatcgaac ccggctgcct cggcgtctgt cacaacggct taaccggtgg tggcgagcgt
     3301 tgaacgcgcc tgaaccgcaa aggggcgcgg cagcttagaa cgaccggaca aggccgatcg
     3361 aga
//
LOCUS       DASVTM010000257         9049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_505560,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000257 DASVTM010000000
VERSION     DASVTM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9049
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505560"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VF633_07050"
     CDS             <1..620
                     /locus_tag="VF633_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8470850.1"
                     /translation="KTIADLKDRPFLLADASITGFWVWLKAKYGFTDEQVRKYTFDPA
                     PFLDDELAVQQGLLTSDPYLVEQAGDIDPKVFLLADEGYPSYAAMVLSPDGFARDNAK
                     ALRSFIAASAEGWRDYMNGDPAAANALIKRDNPDMTDAVLKQARDALNANSIVEGGDS
                     ALYGLGTMTVERWQAFFDSTSSAGVYPPNLNWRNAFTDNYLPGRG"
     gene            827..1879
                     /locus_tag="VF633_07055"
     CDS             827..1879
                     /locus_tag="VF633_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470851.1"
                     /translation="MRNRPNVLTLVMPLLAAAGCLVGSSPASAQQARPGEVMGMRYLS
                     WSGKGADPADGLRRRSGPAPVPTPVAAGNSRYPGIQIATASPSPVRPSRYGVGSIGPT
                     PASMWTGVERSRPPVSAAPPTTAPRMSEPQAAQPDQANPAQTQAEYQAQVQSQYQAWY
                     QSEAQAQYEAQTRAIPQPQQQVVRAAAGSGQQAQPAQVQQPQPPYQQQADAGSPPDYD
                     PMAPRRDAPIFRMQQRSEEPQSQQPGQQPDPVSQQAGSPQGEIKSPVLTQPTAGRTFS
                     GQAYAGAEPPRESARYYSVHRAGGHQPDPIAMPESIYLDNAPIDLAEPPAPPVPGRTV
                     NGRTQVIVPNQDPGLP"
     gene            1876..3015
                     /locus_tag="VF633_07060"
     CDS             1876..3015
                     /locus_tag="VF633_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX8470852.1"
                     /translation="MSAVVARSAATDEIAEAGVLAGSRLPSLIALAQEQSSESRRTLL
                     RELTDHFFGSTIRSAAEDDLYGAVLSDLSADMEAAVRKELSTRFAVRADAPRALIRRL
                     ANDQADVADAVLRASTVLTEADLLGVVRIHGQEHLRAVSSRPEVPEAVSEVIVERGDD
                     ETLGTLLRNDGARLSRTASESAVERARANPALHAATVERSSLPADLLNEMYFVVEARL
                     RQHILEQNAAMDPVLLEAALVAGRSKVATDDGVLPADYAESLAYVQELAAANQLSPQM
                     LARFLRSGSRTPFLISLSQLADVDFHTARQIVERRELDALAVVCRAADLDRALFLTFA
                     VVILGDDGDAMTKAHGYARLYAELTSDQARRTLRFWRMRRGHQAA"
     gene            complement(3085..3513)
                     /locus_tag="VF633_07065"
     CDS             complement(3085..3513)
                     /locus_tag="VF633_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX8470853.1"
                     /translation="MDDKTQLLEMTADIVSAYVGNNSVAANDLPGLISNIHAALSQVS
                     TGVAEAEPEPKEPAVPVRKSISPDFLICLEDGRKFKSLKRHLRTKYDMSPEDYRAKWN
                     LPKDYPMVAPNYAKARSDLAKQMGLGQGGRKPARVTRAKK"
     gene            complement(3675..4892)
                     /locus_tag="VF633_07070"
     CDS             complement(3675..4892)
                     /locus_tag="VF633_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX8470854.1"
                     /translation="MSEHAVIPEGEAPEPAVRARHALLKRLADVVSLPASRINAFERA
                     VTGDLLVEMLRLAAPRERQRVAERLAPLSELPNSLARLLLRDDPAIAGLLIEQCASLT
                     DADLVSCARDTGPEHRLQIANRRGISEVVAETLVGFEEAVVIEALLRNLTARLSQSAV
                     ETIVSLSRQSPVLSALLLRRAELRPSGAYVMFWWCGPEDRKTILQRFAVSREVMQEVV
                     EDVFAMAAEEGWSDPVSRKALQFIERRQRNRGAIAKSPYDSLEQAVAVAAEGGLNRDV
                     ASEIAYLSGVKPLTGAKILGDIGGEPLAILCKATGMTRIDLLNLWRSMRRPEVTTEGL
                     IDPAWERVSITFEMLAVDRAQTVLRYWNWSLSSALTPALLKAIREGENENLDEYSAPE
                     RAAIMALAENFGR"
     gene            complement(4937..5911)
                     /gene="cysK"
                     /locus_tag="VF633_07075"
     CDS             complement(4937..5911)
                     /gene="cysK"
                     /locus_tag="VF633_07075"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX8470855.1"
                     /translation="MSEYDASRHATKGRGKIYDSIIDTIGDTPLVRLPRLSAEYGAKA
                     TVLAKLEFFNPIASVKDRIGVAMVEALEAAGKITADTVLIEPTSGNTGIALAFVAAAK
                     GYKLILVMPESMSIERRKMLALLGAQLELTPAEKGMKGAIARAQELLETTPNSVSPSQ
                     FDNLANPAIHRATTAEEIWNDTNGAVDIVVAGVGTGGTITGVGQALKAKKPSVRMVAV
                     EPTASPVLSGGAPGPHKIQGIGAGFIPSIVDRDMIDSVEQVSNEDSFDMARKVARTEG
                     VPVGISSGAALIATFRLAALDENAGKTIVVIIPSFAERYLSTALFEGL"
     gene            complement(6414..7190)
                     /locus_tag="VF633_07080"
     CDS             complement(6414..7190)
                     /locus_tag="VF633_07080"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8470856.1"
                     /translation="MTSPTAEGLNTPRAWATDLAACAATGVLLGVVGPFGSFFNDTLP
                     VRIAYWTAIFLMSGVVFGAAIRWLWPHARRAKLPIWGWLPVLVLAGSLATGLASRVIA
                     VMLWPGIRQAVGWVEWYGQSVLIGMIYVGLYAVLRMRTAASPEPASDVSEPRILRRLT
                     GRLGRDLICLQMEDHYVRLHTSEGSALVLMSLGKAMAEVGGLEGMQVHRSWWVARHAV
                     EEVIEDGRNLRLRLKTGLEAPVSRAHVARLKAAGWLPGSA"
     gene            7327..8307
                     /locus_tag="VF633_07085"
     CDS             7327..8307
                     /locus_tag="VF633_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HEX8470857.1"
                     /translation="MPQLLRSPRLAGLPAALILLLTPLLGAATLPVAAQAQTVTTTVR
                     LSPDQSFTIPGGADGPTEAGVWLPAPPTDGKPVALIVISHGNGGWYQGHSDTARALAD
                     AGFAVAALTHPGDNFRDMSRSTQLTNRAPQLSLLIDYITGAWKGPVAIDPDRIGAFGF
                     SAGGFTVTSAIGGVSDSRAILTHCVAHPDFFACKLLALQGIDASTWRPEARDDRIKAA
                     AIAAPALGFSFTPASLGTVDIPVQLWQAGDDEILPAPYNVEPIRDRLGGPAEYHRVEG
                     AMHYDFLQACNPGMAAQAPELCHSAPGFDRAAFHQTLNVEVVRFFRDALK"
     gene            8394..>9049
                     /gene="fliI"
                     /locus_tag="VF633_07090"
     CDS             8394..>9049
                     /gene="fliI"
                     /locus_tag="VF633_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518508.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEX8470858.1"
                     /translation="MHNLVAAVEAIDPLVVTGKVAGVSGLLIEARGGLSRLAVGARAE
                     IVRRGATPLPAEVVGFRDSKALLMPFGPVEGVAPGADIKIIGQQASVRPTTAWLGRII
                     DAFGQPIDGKGPLPQGPVPYPLRASPPPAHSRGRVGERLDLGVRSMDVFTTTCRGQRL
                     GIFAGSGVGKSVLLSMLAKQATCDAVVVGLIGERGREVREFIEETLGEEGLKRAIVVV
                     A"
BASE COUNT         1567 a         3094 c         2766 g         1622 t
ORIGIN      
        1 tgaagaccat cgccgacctc aaggaccgcc cgttcctgct cgccgacgcc tcgatcaccg
       61 gcttctgggt ctggctgaag gccaaatatg gcttcaccga tgagcaggtc cgcaaataca
      121 ccttcgatcc cgcgcccttc ctcgacgacg aactcgcggt gcagcagggc ctcctgacat
      181 ccgatcccta tctggtcgaa caggctggcg acattgaccc caaggtcttc ctgctggccg
      241 acgaagggta tccttcatac gcagcgatgg tcctgtcgcc ggacggcttt gcacgcgaca
      301 acgccaaggc cctgcgcagc ttcatcgccg cctccgcaga aggctggcgc gactacatga
      361 acggcgaccc ggccgctgct aacgccctga tcaagcgcga caacccggat atgaccgacg
      421 cggtcctgaa gcaggcgcgc gacgccctga acgccaactc gatcgtcgag ggcggcgact
      481 cggccctcta cggccttggt acgatgacgg tcgagcgttg gcaggcgttc ttcgactcca
      541 cctcgagcgc cggggtctat ccgccgaacc tgaactggcg aaatgccttc acagacaact
      601 acctgcccgg ccgtggctaa cgcggtgact gcggatccag gcgctggtct gtcctgcgca
      661 acattttatt ctgcagtcgc ccattcgtct ccccgacgtc accattagcc agatggtcgc
      721 ctcctgctga ggcgcgatcc cgccaaccgg cgtctggccg ttagtcccgg gttaaggcgg
      781 gctcggctaa cctggccgga cgttccgctg cgaacgggag ccccaattgc gcaaccgccc
      841 caatgttctg accctggtaa tgccgctgct cgccgctgcg ggctgcctcg tcggctcctc
      901 acccgcctcg gcccagcagg cccggcccgg cgaggtgatg ggcatgcgct atctttcatg
      961 gtccggaaaa ggcgcagacc ccgctgacgg ccttcgtcgg cgcagcggcc cggcccccgt
     1021 gccgacccct gtcgctgcgg gcaactcgcg ttatcccggc atccagattg caacagccag
     1081 cccttcgccc gtccgtccct cgcgatacgg agtcggttcc attggtccga cgcccgccag
     1141 catgtggaca ggagtagagc gctcgcgacc tcctgtctcc gctgcgcccc ccacgacggc
     1201 tccccggatg tccgagccgc aagcggcgca gcctgatcag gccaacccgg ctcaaactca
     1261 agcggaatac caggcacagg ttcagtccca atatcaggct tggtaccagt ctgaagccca
     1321 ggcccagtat gaagcccaga cacgtgccat cccgcagcct cagcagcagg tcgtacgcgc
     1381 cgctgccggc tctggacagc aggcccagcc cgcgcaggtt cagcaacctc agccgccgta
     1441 tcaacaacag gcggatgccg ggtcgcctcc tgactatgat cccatggctc cccgccgcga
     1501 tgcgccaatc ttccgcatgc agcagcggag cgaagagccc caatcgcaac agcccggcca
     1561 acagcccgat ccggtcagcc agcaggctgg ttcccctcag ggtgagatca agtcgccggt
     1621 cctgacccaa cccaccgcgg gccggacatt ttcgggtcag gcctacgccg gcgccgaacc
     1681 gcctcgagaa agcgcccgct attactctgt gcatcgtgct ggcggccacc aacccgaccc
     1741 catagcaatg ccggagtcga tctatctcga caacgcgccg atcgatctcg cagagccgcc
     1801 cgcgcccccg gttcctggcc ggaccgtaaa tggtcgcacc caggtcatcg tccctaatca
     1861 ggatcccggg ctcccatgag cgctgtcgtc gcccgatccg cagcaaccga cgagatcgcc
     1921 gaggccgggg tcctcgcggg ctcgcgcctg ccatccctga tcgcgctcgc tcaggagcaa
     1981 tccagtgaaa gccgccggac cttgttgcgc gaactcaccg accatttttt cggctcgacg
     2041 atccgttctg cagcggaaga cgatttgtac ggcgcggtcc tttccgatct gtccgccgac
     2101 atggaggcgg cagttcgcaa ggaactgtcc actcgctttg ccgtccgcgc tgatgctcct
     2161 cgcgccttga tccgccgcct cgccaacgac caagccgatg tcgccgatgc cgtattgcga
     2221 gcttccacag ttcttaccga ggccgatctt ctcggtgtcg ttcgcatcca tggccaggaa
     2281 cacctgcgcg cggtttccag ccgcccggag gtgcctgaag ccgtgtccga ggtgatcgtt
     2341 gagcgcggcg acgacgagac cttgggcacc ttgctccgca acgatggcgc ccgcctctcg
     2401 cgtacagcct ctgagagcgc tgtagagcgc gccagagcca atccagccct ccatgctgcg
     2461 actgtcgagc gcagcagcct gcccgctgat ctgctcaacg aaatgtattt cgtcgttgaa
     2521 gctcggctcc gacagcatat tctcgaacag aatgccgcga tggatccagt gctcctggaa
     2581 gccgcgttgg tcgccggtcg cagtaaggtc gctaccgacg acggggttct gcctgccgac
     2641 tatgcggaga gcctggccta tgtgcaggaa ttggccgccg ccaatcaact gtcgccccag
     2701 atgctggcga ggttcctgcg ctcgggctcg cgaacgccct tcctgatctc actctcccag
     2761 ctagcggatg tcgatttcca cactgcccgc cagatcgtcg aacgtcgtga actcgacgca
     2821 ttggctgtcg tttgtcgcgc cgctgacctg gaccgcgccc tgttcctgac cttcgccgtg
     2881 gtcatccttg gcgacgatgg cgacgcgatg accaaggctc acggctacgc ccgcctctac
     2941 gcggagttga ccagcgacca ggcgcgccgg accctccgat tctggcgtat gcgtcgcggc
     3001 caccaggctg cctgaccgca acgaaaacgc ccgcctcctt tcggaagcgg gcgtctgatc
     3061 tactggaacc gttattgcgg tgaactactt cttggctcgc gtgacgcggg ccggcttgcg
     3121 gccgccctgg cccaggccca tttgcttggc aagatccgag cgcgccttgg cgtagttcgg
     3181 cgcaaccatc gggtagtcct tgggcaggtt ccacttggcg cgatagtctt ccggactcat
     3241 gtcgtatttg gtgcgcaggt ggcgcttcag cgacttgaac ttgcggccgt cttccagaca
     3301 gatcaggaag tccgggctga ttgacttgcg gaccggaaca gcaggttcct tcggttctgg
     3361 ctcagcctcg gccacgccgg tcgaaacctg cgataaagcc gcatggatat tcgagataag
     3421 acccggcaga tcatttgccg cgacactgtt gttgccgaca tacgccgaaa caatgtccgc
     3481 cgtcatctcc agaagctgtg tcttatcgtc cattggcatc ggttccactt ggctctatcc
     3541 ccctgacgat tgacgtcgtc gcgatgtttc tgattccgcc aataatgatg ttcgacagcg
     3601 aaagcaatgc aacaaggata ggggatcgcc aggcgccgat atccagtgtt tcgagcggca
     3661 tcgaactgtt cagactaacg tccgaagttt tccgcaagag ccattatcgc agcacgctcg
     3721 ggcgctgaat attcgtccag attctcgttc tcgccttcac ggattgcttt cagcagagcg
     3781 ggcgtgagcg ccgacgacaa agaccagttc cagtagcgaa gaaccgtctg ggcgcgatct
     3841 accgccagca tctcgaaggt gatcgaaaca cgctcccatg ccggatcgat gaggccttca
     3901 gtcgtgactt cgggacggcg catggatcgc caaaggttga gcaagtcgat ccgcgtcatg
     3961 cctgtggcct tgcagaggat cgccagcggc tcgccgccga tgtcgcccag tatcttggcc
     4021 cccgtcaaag gtttgacacc ggagagatag gcgatttcgg aggcgacgtc acggttcagg
     4081 cccccttctg cggcgacggc caccgcttgt tcgagactgt cgtagggcga cttggcgatc
     4141 gcaccacggt tgcgctggcg gcgttcgatg aactggagcg ccttgcgcga gaccggatcg
     4201 gaccaaccct cttcggcggc catggcgaat acgtcttcca cgacttcctg catgacttcg
     4261 cgcgacacgg cgaagcgctg cagtatggtc ttgcgatctt cggggccgca ccaccagaac
     4321 atcacatagg ctccggacgg ccggagttcg gcgcgccgca gcagaagcgc gctgaggaca
     4381 ggactctggc ggctcaggct gacgatagtc tcgaccgcgc tctgggacag tcgggccgtg
     4441 aggttgcgaa gcagggcttc gatcacgacc gcttcttcga agccgaccag ggtctcggcc
     4501 acgacttctg aaatcccgcg tctgtttgcg atctgcaagc gatgttccgg cccggtgtca
     4561 cgagcacagg agaccaggtc ggcgtccgtc aacgacgcgc actgctcgat cagcaagccg
     4621 gcgatggccg gatcatcgcg cagcaacagc cgggccaggc tgttgggcag ttccgacagg
     4681 ggggccagcc gttcggccac tcgctggcgt tcacgcgggg cggccaaacg cagcatctcg
     4741 accagaaggt cgcccgtcac cgcgcgttca aacgcattga tccggctcgc aggcagcgag
     4801 acgacgtcgg ccagccgctt cagcagggca tggcgcgcgc gcaccgccgg ctccggcgct
     4861 tcgccttcgg gaatgaccgc atgttcggac attcgccgga gactaggcgg ccccggttaa
     4921 ccggtgatga cgcggcctac agaccttcga acaaggcggt cgacagatag cgctcggcga
     4981 agcttggaat gatcacgacg atggtcttgc ccgcgttctc gtccagcgcc gccagtcgga
     5041 aggtcgcgat caacgccgcg cccgagctga tgccgaccgg aacgccctcg gtgcgggcga
     5101 ccttgcgggc catgtcgaag ctgtcctcgt tggacacctg ttcgacgctg tcgatcatgt
     5161 cgcgatcgac gatcgaaggg atgaagcccg cgccgatgcc ctgaatcttg tgtggtccgg
     5221 gcgcgccgcc ggacaggacc ggagacgccg taggctcgac cgcgaccatg cggaccgagg
     5281 gcttcttggc cttcaaggcc tgaccgacgc cggtgatggt gccgcccgtg ccgacgcctg
     5341 cgacgacgat atcgaccgcg ccgttggtgt cgttccagat ttcctcggcc gtcgtggccc
     5401 ggtgaattgc ggggttggcg aggttgtcga actgcgaggg gctcaccgag ttcggcgtcg
     5461 tctccagcag ttcctgggcc cgggcgatcg cgcccttcat gcccttctcg gccggggtca
     5521 gctcaagctg cgcccccagc agggccagca tcttgcgtcg ctcgatcgac atgctctccg
     5581 gcatgacgag gatcagcttg tatcccttgg cagccgcgac aaacgccagg gcgatgccgg
     5641 tgttgccgct ggtcggctcg atcaggaccg tgtcggcggt gatcttgcct gcagcttcga
     5701 gggcctcgac catggccacg ccgatgcggt ccttcaccga cgcgatcggg ttgaagaact
     5761 caagcttggc cagcaccgta gccttggccc cgtactctgc cgacagacgc ggcaaccgca
     5821 ccagaggcgt gtcgccgatg gtgtcgatga tcgagtcgta gatcttgccc ctgcccttgg
     5881 tggcgtggcg gctggcgtcg tattcggaca tggcggatct ccgtctgagc ttcggtcgag
     5941 agtcgaggtc attcaacaga taagccgctt acgcccatcg caaagggtga ctaagcgatt
     6001 tctcgctgac acgcctgcga gatttagtgt cgccgacgcg gatcgggtcg agagtctcgc
     6061 cgccacagaa agcgctcgaa agcgatactc tggagactct ggacccgggc gcttgcgatt
     6121 tcggggcgcg cagcgcggca atcaaggtcg gaacctcaga cacggtgatg cgtccggttg
     6181 agcggcccgc cttcatcgga cgggcgcaag aatatcgatg tcaaagaccg cggcgcgccg
     6241 cacgacgcgg caagctgaca gtgtaccacg ccccggaacg aggggtgtcg attgggatac
     6301 gccgggatcg ggagtctccg gtcagggccg aaatgtctcc agagtattgg tatccaatgt
     6361 ctccaccgtc gggcgccaac aagatgctat tgcagacgca cagcttgggc ggatcaggcg
     6421 gatcccggta gccaacccgc cgccttcaat cgcgccacat gagcgcgcga gacgggcgct
     6481 tccagccccg tcttcagccg cagacgcaga ttgcgtccgt cctcaatgac ctcctcgacc
     6541 gcatgccggg caacccacca cgagcggtgc acctgcatcc cttccaggcc tccgacctca
     6601 gccatggcct tgccgagcga catcagcaca agagccgacc cttcggaggt atgcagccgc
     6661 acataatgat cctccatctg cagacagatc agatcacggc ccagccgtcc cgttaaccgc
     6721 cgcaggatcc ggggttcgga gacatccgag gcgggctctg gtgaggcagc agtcctcatc
     6781 cgcagcacgg cgtagagccc tacatagatc atgccgatca gcaccgactg cccgtaccac
     6841 tcgacccagc ctacggcctg gcgaatcccc ggccagagca tcaccgcgat cacccggctc
     6901 gctaagccag tcgcaaggct tccggccagt accaggaccg gcagccagcc ccagatcggc
     6961 agtttagcgc gtcgggcgtg cggccaaagc cagcggatgg ctgcgccgaa caccacgccg
     7021 ctcatcagga aaatcgccgt ccagtatgcg atccgcaccg gcagtgtgtc gttgaagaag
     7081 ctgccgaacg ggccgaccac gcccagcagg acgccggtgg ccgcacaggc cgccagatcg
     7141 gtggcccatg cgcgcggcgt gttcagtccc tcggcagtcg gtgacgtcat gcttgctctt
     7201 atagccgtgc ttttgtcgcc aaaaccattc gccgaactgc gtacccgttc gcccatcggc
     7261 gaaacgaggc tggcaggcgg tctcggccca cgaaacaggg gatcatcctc aacgctggaa
     7321 cttaccgtgc cccaactcct ccgatccccc cgcctcgccg ggctccccgc tgccctgatc
     7381 ctgctcctca ccccgctgtt aggggccgcg acactgcctg tcgccgccca agcccagacg
     7441 gtaactacga cggtaagact gtcgccagat cagtcgttca ccattcccgg cggcgcggac
     7501 ggtccgaccg aagccggcgt atggctcccc gccccgccca ccgatggaaa gccggtcgcg
     7561 ttgatcgtca tctctcacgg caacggaggc tggtaccaag gtcactcgga caccgcccgc
     7621 gccctcgccg acgccgggtt cgccgtcgcc gccctgacgc atccgggcga caactttcgc
     7681 gacatgagcc ggtccaccca gctcacaaac cgtgcccccc aacttagcct gctgatcgac
     7741 tacataaccg gtgcctggaa aggtcctgtc gccatcgatc cggatcgcat cggcgcgttc
     7801 ggcttctcgg cgggcggctt taccgtcacc tcggccatcg gcggcgtgtc ggattcccga
     7861 gccatcctga cccattgcgt cgcgcacccc gacttcttcg cctgtaagct gctggcgctt
     7921 caaggcatcg acgcttcgac atggcgaccc gaggcgcggg acgaccggat caaggccgcc
     7981 gccatcgccg cgccggccct tggcttctcg ttcacccccg ccagcctcgg caccgtcgat
     8041 atcccggtcc agctctggca ggcgggtgac gacgaaatcc tgcccgcacc ctacaatgtc
     8101 gagcccattc gagatcgcct tggtggcccg gctgaatacc accgggtcga gggcgcgatg
     8161 cactatgact tcctgcaggc ctgcaatccg ggcatggccg cccaagcgcc tgaactctgc
     8221 cactcggcgc ccggtttcga ccgcgcggcc ttccatcaga cgctgaatgt agaggtcgtc
     8281 cgcttctttc gggacgccct gaaatagcgt tcgcccccaa cgcatgaggc cgaccttcac
     8341 cggtcattaa ccacgatggg ccattcgtta acgcctcatt acagggcttt tcgttgcaca
     8401 atctcgtcgc cgccgtcgaa gctatcgatc cgctggtggt gaccggaaag gtcgccgggg
     8461 tcagcgggtt gctgatcgag gcgcggggcg gtctgtcgcg gctggccgtc ggggcgcgag
     8521 ccgagatcgt gcggcggggt gcgacgccct tgcccgccga ggtcgtgggt ttccgggact
     8581 ccaaggcctt gctgatgccg ttcgggccgg tggagggcgt ggcgccgggc gcggacatca
     8641 agatcatcgg ccagcaggcc tcggtgcggc cgacaacggc ttggctgggg cggatcatcg
     8701 acgctttcgg ccagccgatc gacgggaagg ggccgttgcc gcaggggcct gtgccctatc
     8761 ccctgcgcgc ctcgccaccg cctgcgcatt cacgcggccg ggtcggtgag cggctggatc
     8821 tcggcgtgcg ctcgatggac gtgtttacga ccacctgccg gggccagcgg ctgggcatct
     8881 tcgcggggtc gggggtcggc aagtcggtgc tgctgtctat gctggccaaa caggcgacat
     8941 gcgacgcggt cgtggtcggg ctgatcggcg aacggggccg ggaggtccgc gagttcatcg
     9001 aggagacgct gggcgaagaa ggcctgaagc gcgccatcgt cgtggtcgc
//
LOCUS       DASVTM010000258         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_717172,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000258 DASVTM010000000
VERSION     DASVTM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717172"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VF633_07095"
     CDS             <1..808
                     /locus_tag="VF633_07095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8470859.1"
                     /translation="VVGFCHSDPAALAALHFGEWAKKPVEKRWAKLFTQFDRVVAPSR
                     FIARRLEEAGVSNIVIRPLGVEIDAFRPDRRDRDWLLKKLGLSSDARLLCFAGRPARE
                     KNIDVLIEAVQKLGAPYHLVLVGAGQGMPEEDRVIALPYEKDPRAVARIIASCDAFVH
                     ANDKEPFGLIVLEAMACGRPVVGVNAGGVAETVTNDVGQLAASADPTEYAAAIEALFA
                     RDIEAIGAAARVHTVERFAWNRVFEDLCMVYADVSGESAFVQPSSEWAVH"
     gene            complement(823..1659)
                     /locus_tag="VF633_07100"
     CDS             complement(823..1659)
                     /locus_tag="VF633_07100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX8470860.1"
                     /translation="MARHATPADLQIKPRDLHIDREVATPRWWLNNDPFGTAVMNALS
                     LTFPDGERFFIQSVRRFAKDAPPKLAADIRAFTVQEGAHTREHMAFNAITARAGYETA
                     EIEAYVNRRLDIARSRPPIGQLAATMALEHFTAAFAHRLLADPDLLKGAPVDLARLWR
                     WHSIEEIEHKGVAYDVFLHATRNLSPMKRWSIRRWAMLLTTLLFTRTVRETTLMLLKQ
                     DGIVGWRARFGLFRWLWVTPGLYRRMIGDYASFYMPDFHPWQVDDRNLIAEAEAGLAQ
                     AA"
     gene            1706..>2000
                     /locus_tag="VF633_07105"
     CDS             1706..>2000
                     /locus_tag="VF633_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8470861.1"
                     /translation="MSTNAGTLLPVKRRRGAVAREEALDAARDLLLSGGPAAVTLKAV
                     GERMGVGHANLIHHFGSAAGLQGALMDRMVRDLAERIEAGLGQGPERIEPGRLM"
BASE COUNT          344 a          649 c          653 g          354 t
ORIGIN      
        1 ggttgtcggt ttctgtcact ccgaccccgc tgcgctcgcg gcgcttcact tcggcgaatg
       61 ggccaaaaag ccggtcgaga agcgttgggc aaagctgttc actcaattcg accgtgtcgt
      121 cgccccgagc cgtttcatcg cccgtcgtct tgaagaagcg ggcgttagca atatcgtcat
      181 ccggcccttg ggcgtggaga tcgacgcatt ccgcccggac cgccgcgacc gtgattggct
      241 gctcaagaaa ttgggcctca gctccgacgc ccgcctgctg tgcttcgccg gtcgcccggc
      301 gcgcgagaag aacatcgatg tcctcatcga ggcggtccag aagctgggcg cgccctatca
      361 tctggtgttg gttggagcgg ggcaggggat gcccgaggag gatcgggtca tcgccctgcc
      421 gtatgagaag gacccgcgcg ccgtggcccg gatcatcgcc agctgcgacg ccttcgtcca
      481 cgccaacgac aaggagccgt tcggcctgat cgtgcttgag gccatggcct gcggacgacc
      541 cgtcgtcggg gtcaacgccg ggggcgtcgc cgaaaccgtc actaatgacg tcggccagct
      601 tgccgcctcc gccgacccga ccgaatacgc cgccgcgatc gaggccctgt tcgcgcgtga
      661 catcgaggcc atcggcgcgg ccgcccgtgt ccacaccgtc gaacgcttcg cctggaaccg
      721 cgttttcgag gacctgtgca tggtctacgc agacgtcagc ggcgagtcgg cctttgtcca
      781 gccctcaagc gagtgggccg tccactaagg cgtcgcagtt gctcaggcgg cttgagccag
      841 acccgcttcg gcctccgcaa tcagattacg gtcgtcgacc tgccacggat ggaagtcggg
      901 catgtagaag ctggcgtaat cgccgatcat ccggcgatag agacccggcg tgacccacag
      961 ccagcgaaac aagccgaacc tcgccctcca gccgacgatg ccgtcctgct tcagcagcat
     1021 caacgtcgtc tcgcgcaccg tccgggtgaa aagcagcgtc gtcagcagca tggcccagcg
     1081 ccggatgctc cagcgcttca tcggcgacag attgcgcgtg gcgtgcagga agacgtcata
     1141 ggccacgccc ttgtgctcga tctcttcgat cgaatgccag cgccacagac gcgccagatc
     1201 gaccggtgcc cccttcagca ggtcgggatc agccagcagc cggtgtgcga acgccgcggt
     1261 gaaatgctcg agcgccatcg tcgccgccag ttgcccgatc ggcggccgcg aacgggcgat
     1321 gtcgaggcga cggttcacat aggcctcgat ctcggctgtc tcgtatcccg cccgggcggt
     1381 gatggcgttg aaggccatat gctcgcgggt gtgtgcaccc tcctgaaccg tgaaggcgcg
     1441 aatgtcggcc gccagcttgg gcggagcgtc cttggcgaaa cgccggaccg actggatgaa
     1501 gaaccgctcg ccatcgggga aggtcaggct cagcgcgttc atcaccgccg tgccgaacgg
     1561 atcgttattc agccaccagc gtggcgtggc gacctcgcgg tcgatatgca ggtcgcgagg
     1621 cttgatctgg aggtcggcgg gtgtggcgtg tctggccatg gttctgctcc tcgtattgaa
     1681 gtcagaatga ggttaactga cattaatgtc aactaatgcc ggaaccctac ttcccgtcaa
     1741 gcgtcgacgc ggcgccgtcg cacgcgagga agcgctcgac gctgcgcgcg acctgctgct
     1801 ctcaggtggt cccgcagcgg tgacgctcaa ggcggtcggc gagcgcatgg gcgtgggaca
     1861 cgccaacctg atccatcatt tcggctcagc tgccggtctg cagggcgccc tgatggaccg
     1921 gatggtgcgc gatctggccg agcgaatcga agccggtctt ggacaaggtc ctgagagaat
     1981 cgagcccgga cgcctgatgg
//
LOCUS       DASVTM010000259         6539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_395100,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000259 DASVTM010000000
VERSION     DASVTM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395100"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VF633_07110"
     CDS             <1..574
                     /locus_tag="VF633_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX8470862.1"
                     /translation="DTALIGDSAILYLEGYLFDPAPARAAFEAAAAAAHAAGRKVAIT
                     LSDTFVVARWRAELLAFIEASADIVLANESELGALFETEDFDAAADKLASMVEIAAVT
                     RGAEGSVIISGDERVSVAAYPVAKVVDTTGAGDQYAAGFLLGLARGLSLEDAGKLGSL
                     AASEVIAHWGPRPMVKLEELAQQNGLTLQA"
     gene            complement(581..1084)
                     /locus_tag="VF633_07115"
     CDS             complement(581..1084)
                     /locus_tag="VF633_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX8470863.1"
                     /translation="MTPPKVRKAARIVPGGPLPADPPASVALTGPSARVRPVISPKTP
                     PKPVSPAPAASHPGKSAPEELEPRRQRRLSRERDPIEDRIDLHGFGRFEAEDQLRAFL
                     SGAQARGLRAVLVITGQGRIGGGVIRASIHEWLQSAALRPIVSGFAIAHRRHGGDGAI
                     YVTLKRR"
     gene            complement(1214..1453)
                     /locus_tag="VF633_07120"
     CDS             complement(1214..1453)
                     /locus_tag="VF633_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8470864.1"
                     /translation="MAIRIQLDRILLERRMSLTELADRVGVTLANLSILKTGKARAIR
                     FSTLDILCRELNCQPGDLLVHEPGPQEIGPDDGAD"
     gene            complement(1469..2092)
                     /locus_tag="VF633_07125"
     CDS             complement(1469..2092)
                     /locus_tag="VF633_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX8470865.1"
                     /translation="MRLPTLENLRGRLPAGGFKPRFAIPPLRGPLRTLGPGSVASLLK
                     IALDVAYVLLMLITAVLLVAFVAAVFIPISNYNITVSDHDGGLQQPLTRPLLLFGLGA
                     LSAWFGGFLMILRNLRLIFRTLTAGDPFQPENVRRLRGIGLTLATVTAGVWVAQGMVA
                     ARLVPGVMDPQGFGELLTPIFSVLVVFVLAEVFREGARLRRESELTI"
     gene            2612..3214
                     /gene="rdgB"
                     /locus_tag="VF633_07130"
     CDS             2612..3214
                     /gene="rdgB"
                     /locus_tag="VF633_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX8470866.1"
                     /translation="MKLKLIKGMRLIAATHNPGKVPEIAALLDGNYEIVTAGELNLPE
                     PDETETTFGGNAMLKARHAARLSGEVCLADDSGLSVAALDGAPGIFSARWAGPGKDFA
                     LAMQKVEQRLEEAGAKDHRAWFTSSLAVAWPDGPCVVVEGEIHGQLTFPARGTRGFGY
                     DPIFIPDGFDQTFGEMEPAAKDAMSHRARAFAKLKAALID"
     gene            3207..4373
                     /gene="hemW"
                     /locus_tag="VF633_07135"
     CDS             3207..4373
                     /gene="hemW"
                     /locus_tag="VF633_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX8470867.1"
                     /translation="MTDRFDPDPLDPGSDVAVYVHWPYCARICPYCDFNVVRDRGRTE
                     EQAGLVAAILTDLDAQAALVGERRLASVFFGGGTPSLMPPDAVAAVIEGIHARFPSAG
                     PIEITLEANPTDAEASHFAALADAGINRLSLGVQSLDDAALRFLGRNHSAAEARRAIA
                     VAGAAFDRLSIDLIYARPDQSVADWATELTTAVDLGFEHVSPYQLTIEPTTAFGRAAA
                     RGDWTPPDEDLSAALYETTQAVLEAAGFEAYEVSNHARGIAARSAHNIHVWRGGDYLG
                     LGPGAHGRLTLHGVRNATVAHRRISDYVTGVAAASPWAEREVLDAEGAAEERILLGLR
                     TVEGAESSAFEMLRLSTKAAELIADAFLIHEQGRVIATAKGRPVLDGVLKALLT"
     gene            4416..5303
                     /gene="rsmI"
                     /locus_tag="VF633_07140"
     CDS             4416..5303
                     /gene="rsmI"
                     /locus_tag="VF633_07140"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX8470868.1"
                     /translation="MSESSATPSPFPPTAPPSRLVSPGLYLVATPIGNLRDITLRALD
                     VLAAADLVLAEDTRVTGKLLSAYGLKAKLERCDDHASARAAEIAIERVREGKVVALVS
                     DAGTPVISDPGFVVARAFIAADLPIHPIPGASSLLAALCIAGQPSDRFLFAGFLPPRS
                     GARKTMLAELKQGRQTLVFFESGPRLAASLADMAEVLGPRPAAVTRELTKLYEEAIRG
                     TLDELAVDPRCDGPKGEIVVVIGPGAVEIASAADADAALIEALARLPTGEAASEVSQA
                     LDLPRKALYRRALELQGKG"
     gene            5300..5722
                     /locus_tag="VF633_07145"
     CDS             5300..5722
                     /locus_tag="VF633_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX8470869.1"
                     /translation="MKPSRVRAPKVAARPKAAWRQALGSRAFRQGHASEWIAAAFLML
                     KGYQILGFRLKGRGGEIDILARRGRVLAVVEVKRRSTMEAALAALTPDQHQRLLVSGR
                     AVLHGRPSLAGHELRIDMVALAPGRFPRHVSAISFPGS"
     gene            5756..>6539
                     /locus_tag="VF633_07150"
     CDS             5756..>6539
                     /locus_tag="VF633_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX8470870.1"
                     /translation="MTREEAEELLVEAGQADDDAFPLLEAAIACAIHDYPLRDPQPVR
                     TLARNAAERLKERIGSESPDDALAETMAGDLRLNGDLLHYEDPANTDIIAVAERRRGL
                     SAALAVFYLDAAKRAGLEAQGVDFPNHFLVRVETPAGPVALDPFSEGRIVMPSELTRR
                     ALRAGLTPHVADRLDLLMAPVSDRQALIRLQNVLFSRAIRAEDYQGAERSALRRALLD
                     PEDHRPWLDVASAREKQGALNGALEALYRARIVGGQEIVVQAR"
BASE COUNT         1113 a         2174 c         2163 g         1089 t
ORIGIN      
        1 cgacacggcc ctgatcggcg acagcgccat cctgtacctc gaaggctatc tgttcgaccc
       61 cgccccggcc cgcgccgcct tcgaagccgc cgccgccgcc gcccacgcgg ccggccgcaa
      121 ggttgcgatc accctgtcgg acaccttcgt cgtcgcccgc tggcgcgccg aactgctggc
      181 cttcatcgag gcctcggctg acattgtcct ggctaacgaa agcgaactgg gcgcgctgtt
      241 cgagaccgaa gatttcgacg ccgccgccga caagctggcc tcgatggtcg agatcgccgc
      301 cgtgacccgg ggcgccgagg gatctgtgat catcagcggc gacgaacgcg tttctgtcgc
      361 cgcctatccg gtcgccaagg tcgtcgacac cacgggcgcc ggtgaccagt acgccgccgg
      421 tttcctgttg ggtctggcgc gcggcctttc gctcgaagac gccggcaagc tgggttcgct
      481 ggccgcttcg gaagtgatcg cccactgggg tccacgcccg atggtcaaac tggaagagct
      541 ggcgcagcaa aacgggctta cgctgcaggc ctgatcggcc tcagcgccgc ttaagcgtga
      601 cgtagatggc cccgtcgccg ccgtggcgac ggtgggcgat ggcgaagccc gagacgatcg
      661 ggcggagtgc ggccgactgc agccactcgt ggatcgaggc gcggatgacg ccgccgccta
      721 ttcgtccctg acccgtgatg accagaacgg cccgcaggcc gcgcgcctgt gcgcccgaca
      781 ggaaggcgcg aagttgatcc tccgcctcga accggccgaa gccgtgcagg tcgatgcgat
      841 cctcgatggg atcccgttca cgcgacagcc gccgctgacg acgcggctcc agctcttcgg
      901 gcgccgactt gccgggatgc gatgcggcag gcgctggcga aacaggtttg ggcggcgtct
      961 tcggactgat caccggtcga accctggccg atggtccggt cagggcgaca ctggcgggag
     1021 gatcagctgg aagcggcccg ccgggaacga tccgcgcagc cttgcggacc tttggagggg
     1081 tcacagagcc cgatacgcgc gtccagatcc gccggtcctc cgacgtcaga gtccccgatc
     1141 gtttttcgga ggatttcttc tccgaaggcc tcttttcagg agggggcgcg cccggaggac
     1201 gtttgaccat ggctcagtcg gcaccatcgt cgggaccgat ctcctgcggg cccggctcat
     1261 ggaccagaag atcaccgggc tggcagttca gttcgcggca caggatgtcg agcgtcgaga
     1321 agcggatcgc acgcgctttg ccggtcttga ggatcgacag attggccaag gtcaccccga
     1381 ctcggtcagc gagctcggtc agcgacatgc gccgttcgag caggatgcgg tcgagctgta
     1441 tgcgaatggc catggaaagt gtgctcgatc agatcgtgag ttcggactcg cgacgcaacc
     1501 gggcgccctc gcggaacacc tcggccagca cgaagacgac aaggacggaa aagatggggg
     1561 tcaatagctc gccgaacccc tgcgggtcca taaccccggg caccagccgg gccgcgacca
     1621 tgccctgagc gacccagacg cccgccgtca cggtcgccag cgtcaggccg atgccgcgca
     1681 atcgcctgac gttctcgggc tggaaggggt cgccggcggt caatgtgcga aagatgaggc
     1741 gcaggttgcg caggatcatc aggaagccgc cgaaccaggc cgacagggcg ccaaggccga
     1801 acagcaacaa cgggcgcgtc aggggctgct gcaggccgcc gtcatgatcg ctgacggtga
     1861 tgttgtagtt ggaaatggga atgaagacgg ccgcgacgaa ggcgaccagc agaaccgccg
     1921 tgatcagcat cagcagcaca taggctacgt cgagagcgat cttcagcagg ctggccaccg
     1981 atccggggcc gagggtccgc aaggggccgc gaagaggagg gatagcgaag cgaggcttga
     2041 agcctcctgc gggaagccga cctctcagat tttccagggt cgggaggcgc atcggccgtt
     2101 tcgctcaggc cggaaggaag ggcgcgaatg ccgcgagggg tccggcgaac cccgtcgcgg
     2161 tcttattcat cgcgatcttc atgggtctcg tcaaagcctc acaggacgca tttaccgtgg
     2221 accgggggtc tggaagaccc ccgtcggtca aatccttgcg tcggaaccga cgcaaagcgc
     2281 ggtatcagtc ctagaatgct tgggtgagga tagcgatcat ggcggtgggc agggcggcga
     2341 gaagtgcggc attgaccaga acctggccga ccttgtccat gaagagcgag gcgttcatcg
     2401 tgttcatcgt agttctcctg aatgtgggtc atttcatgcc cacttggtta tgaccgcgag
     2461 gtaaagattg ttcgtttccg tcaatctctg gaacggtgcg gcccccgttc gtgagaacat
     2521 agataggacg tgcaatgacg aaggtcacgt tacatatcgt ttaacgatat taaactttgg
     2581 caatggattg ttacgagagg cgtgatccac gatgaaacta aagcttatca aggggatgcg
     2641 gctcatcgcg gcgacccaca acccgggcaa ggtgccggaa atcgctgccc tgctggatgg
     2701 aaattacgag attgtcacgg caggagagct gaatctgcct gaaccagacg aaacggagac
     2761 cactttcggc ggcaacgcga tgctgaaggc gcgccacgcg gctcggctgt ccggcgaagt
     2821 ttgcctagcc gatgactctg gcctgtcggt ggccgctctc gacggagcgc ctggcatttt
     2881 ttctgcgcgc tgggccgggc caggcaagga ttttgctctg gcgatgcaga aggtggaaca
     2941 gaggcttgag gaagccggcg ctaaggatca tcgcgcctgg ttcacatcct cgctggctgt
     3001 ggcttggcca gacggcccct gtgttgtggt cgagggcgag atccacggcc aactgacctt
     3061 cccggcacgg gggacgcgcg gcttcggcta cgatccgatc ttcatccctg acggcttcga
     3121 ccagacgttc ggcgagatgg agccggcggc caaggacgcc atgagccacc gcgcccgcgc
     3181 cttcgccaag ctcaaggccg ccctgattga ctgatcgttt cgaccccgac cctttggacc
     3241 ccggaagcga cgtcgccgtc tatgtccact ggccctactg cgcccgcatc tgcccctatt
     3301 gcgacttcaa cgtcgttcgg gatcgggggc ggaccgagga acaggcgggg ctggtcgcgg
     3361 cgatcctgac cgatctggac gcgcaggccg cgctggtcgg cgagcggaga ctggcctcgg
     3421 tcttcttcgg aggcggcacg ccatccctga tgccgccgga cgccgtggcg gcagtgatcg
     3481 agggcatcca tgctcgcttt ccgtcggcag gaccgatcga gatcacgctc gaggccaacc
     3541 ctaccgacgc cgaagccagc catttcgcgg cgctggctga cgccgggatc aaccggctat
     3601 cgcttggagt gcagtcgctc gacgatgcag cgctgcgttt cctcggccgg aaccattcgg
     3661 cggcggaagc ccggcgcgcc atcgcggtcg cgggcgccgc cttcgatcgg ctgtccatcg
     3721 acctgatcta tgctcgtccc gaccagtcgg tcgctgactg ggccaccgag ctgaccaccg
     3781 ccgtcgacct cggcttcgaa cacgtctcgc cgtaccagct gaccatcgag cccacgactg
     3841 cgtttggacg agccgccgcg cgtggcgact ggaccccgcc cgacgaggac ctgtccgccg
     3901 ccctctatga aaccacccag gccgtgctgg aggccgccgg gttcgaggct tatgaggtgt
     3961 ccaaccatgc ccgggggatc gccgcacgct cggcgcataa catccatgtc tggcggggtg
     4021 gcgactacct cggcctcggc ccaggcgccc acgggcgcct gacgcttcac ggcgtccgca
     4081 acgccaccgt cgcccaccgc cggatcagcg actacgtcac gggagtggct gccgcgtcac
     4141 cttgggccga acgcgaggtg ctggacgccg aaggcgccgc cgaagagcgg attctgttgg
     4201 gcctgcgtac ggttgagggc gccgaaagct ccgccttcga aatgctgcgc ctctccacca
     4261 aggccgccga attaatcgcc gacgcctttc ttatccacga acagggccgc gtcatcgcca
     4321 ccgccaaggg tcgccccgtt ctggacgggg tgctgaaggc cctgctgaca tagagcgctt
     4381 ggcctgaagg ctcagggctg gcacggtccc gcgcaatgtc cgagtcctcc gcaacacctt
     4441 cgcccttccc cccgaccgcc ccgccgtcgc gcctcgtttc gcccggcctc tatctggtgg
     4501 cgacgccgat cgggaacctg cgcgacatca ctttgagagc gctcgacgtg ctcgccgccg
     4561 cagatctggt gctggccgaa gacacccgcg tgaccggcaa gctgctgtcc gcctatggcc
     4621 tcaaggccaa actggagcgc tgtgacgacc acgcatcagc ccgcgccgcc gaaatcgcca
     4681 tcgaacgcgt tcgcgaaggc aaggtggtgg ctctggtttc tgacgcaggc acgccggtga
     4741 tctcggaccc cggcttcgtc gtcgcgcgcg ccttcatcgc cgccgacctg cccatccatc
     4801 cgatccccgg cgcctctagc ctgctggctg cgctgtgcat cgccggccag cccagcgacc
     4861 ggttcctttt cgcgggcttc ctgccgccca ggtctggggc gcggaaaacc atgctggcag
     4921 agctcaaaca gggccgccag acgctggtat tcttcgaaag cggcccgcgt ctggccgcca
     4981 gcctcgccga catggccgag gtgctgggcc cccgccccgc cgccgtcacg cgcgagctga
     5041 ccaagcttta tgaggaggcg atccggggca cgctggacga actggccgtc gatcctcgat
     5101 gcgatggtcc aaagggcgag atcgtcgtcg tcatcgggcc cggcgcggtc gagatcgcct
     5161 cagccgccga cgccgacgcc gcactgatag aggctctggc ccgcctgccg acaggagagg
     5221 ccgcgtccga ggtctcacag gcgctcgacc tgcccagaaa ggcgctctat cgccgtgcgc
     5281 tggagcttca aggcaaggga tgaagccgtc ccgagttcga gcgcccaagg tcgctgctcg
     5341 ccccaaggcg gcgtggcggc aggcgttggg aagccgggcg tttcggcagg gtcatgcgtc
     5401 ggagtggatc gccgccgcct tcctgatgct gaaaggctac cagattctcg gttttcgcct
     5461 gaaggggcgc ggcggagaga tcgacatcct cgcgcggcgc ggtcgtgtcc tcgccgtcgt
     5521 cgaggtcaag cgacgctcca cgatggaggc cgcgctggcc gccctgaccc cggatcagca
     5581 tcaacgactg ctcgtctcgg gccgggcagt gttgcatgga cgacccagct tggccggaca
     5641 tgaattgcgg atcgacatgg tggcgctggc cccgggacga tttccgcgcc atgtttccgc
     5701 cataagtttt ccggggtcct gattttcagg attgggcccc actgaggggc accacgtgac
     5761 gcgggaagaa gcggaagagc ttctggtcga ggccggtcag gccgacgacg acgccttccc
     5821 cttgctggag gcagcaatcg cctgcgccat tcacgactat ccgctccgcg atccccagcc
     5881 agtccgcacc ctcgcccgca acgccgccga gcggctcaag gaacggatcg gcagcgaaag
     5941 ccccgatgat gccctggcag agaccatggc tggagatttg cgtctgaacg gcgacctgct
     6001 ccactacgag gatcctgcca acaccgacat catcgccgtg gcggagcgca ggcggggcct
     6061 gtccgcggcg ctggccgtct tctatctgga tgcggccaag agggcgggcc tggaggcgca
     6121 gggcgtcgac ttccccaacc atttcctggt gcgggtcgag accccggcgg gtcccgtggc
     6181 tctggatccc ttcagcgagg gcaggatcgt catgccgtcc gaactcaccc gtcgcgcact
     6241 gcgggccggc ctgaccccgc acgtcgctga caggcttgac ctgttgatgg cgcccgtcag
     6301 cgatcgtcag gccctgatcc ggctgcagaa cgtgctgttc agccgcgcca tccgcgctga
     6361 agactatcag ggagccgaac gttcggccct gcgccgcgcc ctgctcgatc ccgaggatca
     6421 ccggccctgg ctggacgtcg cctccgcccg cgagaaacag ggcgccctca atggcgcact
     6481 ggaagccctg taccgcgccc gcatcgtggg cggtcaggag atcgtggtgc aggcgcgcg
//
LOCUS       DASVTM010000260         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_293253,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000260 DASVTM010000000
VERSION     DASVTM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293253"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VF633_07155"
     CDS             <1..897
                     /locus_tag="VF633_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8470871.1"
                     /translation="GSEDKSGAVQAGARDSPEPPFPPQHQPKPGDEAALDPAPMYDAP
                     FWKGSGKLDGFAALITGADSGIGRSVAVLFAREGCDVAICHLDEASDAEETKRAVEAE
                     GRRAIILQGDVADPAFSKMAVEATLEAFGRIDVVVPNAAFQEHVDAFEDLTFEHFDRT
                     LKTNLYGYFNLTHAAVPHMKNGGAIVMTGSVTGIMGNKDLLDYSMTKGGIHAFARSLG
                     THLAPRGIRVNVVAPGPVWTPLNPSDKNAKDVSKFGADTVMKRPAQPEEIAPAYVFLA
                     SPQMSSFITGEILPIIGGYSGG"
     gene            complement(913..1200)
                     /locus_tag="VF633_07160"
     CDS             complement(913..1200)
                     /locus_tag="VF633_07160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8470872.1"
                     /translation="MTDFRDVPAEQRFEQGFTGADGEMHLVFADYASQGSQRAILHVE
                     ADDELRGTGAAGKFMQSLADHARAEGLKLTPRCSYAVAWFKRHKDYQDILV"
     gene            complement(1322..1711)
                     /locus_tag="VF633_07165"
     CDS             complement(1322..1711)
                     /locus_tag="VF633_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470873.1"
                     /translation="MTDTQQPRLESGHTSEGKPAAIIAWALFILSIPSANLLVIIGLI
                     VAYAGRGSATGLARQHIDAQIALFWSVFWWTIGLWVIVAISSLLSTILIGIPFLLLAL
                     LLLFVLHVWFTVKAILGLLNLLGDRAP"
     gene            complement(1743..2621)
                     /locus_tag="VF633_07170"
     CDS             complement(1743..2621)
                     /locus_tag="VF633_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470874.1"
                     /translation="MAKPGDPYSPQEGTPQGPNDDDLFRDDTPPATPQSQRPTWAAPV
                     RPAPESTTEVSPEPDPHAHDDLIGFTSFASLAGTGRNPPKPAIEPPSATEPVAQEPAA
                     TQPVMPEPVVAPFPEPDPLIHSPKFTAPSRESLRVDEAFLKPSPVPAWAVETPPSTRP
                     VPSSTAGRTAFGRADGPRAEAVAPEGAMGLYTIYALILFAVPTFGLAAMIGLFMVWTK
                     SKPDQPLARSHFEFQTRTLWIAAIVGVVGVVLIAVNLGVFVLFLMAVWVIVRGAWGVM
                     TLAAGKPVRNPQTWLL"
     gene            complement(2747..3148)
                     /gene="folB"
                     /locus_tag="VF633_07175"
     CDS             complement(2747..3148)
                     /gene="folB"
                     /locus_tag="VF633_07175"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX8470875.1"
                     /translation="MTAFLPDIAAFPSIDAVRREGLTIFVRGLSVEAGIGVYDHEQGR
                     LQTLVIDVTLDLGPHPVEKLSDTINYETIAQAARDIIAQGHVGLVESFAERLALACLE
                     DVRVRRVTVRVEKPGALAGAIAPGCELTYAR"
     gene            complement(3152..3904)
                     /locus_tag="VF633_07180"
     CDS             complement(3152..3904)
                     /locus_tag="VF633_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8470876.1"
                     /translation="MTEARAALVTGGGKRIGRAICLALAEAGHDVAIHYRSEPEEAEA
                     VADAVHALGRRAVILKADLTDEDQVKALIPAAVAALGPLAVLVNNASVFEDDRVGDLS
                     RETWDKHLDTNLRAPVVLAEAFAAQAPNGSVIINLLDQRVLKPDPRFFSYALSRNGLW
                     WATQTLAQALAPRIRVNGVGPGPTLPSVHQTEAEFAAEAAGTLLQKPGSPEAVAAAVL
                     WLVGADMVTGQMIAVDGGQHLAWQTPDILDAE"
BASE COUNT          712 a         1223 c         1409 g          682 t
ORIGIN      
        1 ggcagcgagg acaagagcgg tgcggttcag gccggagccc gcgattctcc tgagcctcct
       61 ttcccgcctc agcatcagcc caagcccggc gatgaggccg cactcgatcc tgcgccgatg
      121 tatgacgcgc ccttctggaa aggctctggc aagctcgacg ggttcgcagc gctgatcacc
      181 ggcgcggact caggcatcgg caggtcggtg gctgtgttgt tcgcgcgcga aggctgcgat
      241 gtcgccatct gccatctcga tgaggcgagc gacgccgaag agaccaagcg ggcggtcgag
      301 gccgaggggc gcagggccat catactgcaa ggcgacgtcg ccgatccggc cttctcgaag
      361 atggcggtcg aggcgacgct ggaggccttc gggcgcatcg acgtagtcgt accgaacgcc
      421 gcctttcagg agcatgtgga cgccttcgag gacttgacgt tcgagcattt cgaccgaacg
      481 ctgaagacca acctctatgg ctatttcaac ctgacgcatg ccgccgttcc gcacatgaag
      541 aatggcggag ccatcgtcat gaccgggtcg gtgacgggga tcatgggaaa caaggacctg
      601 ctggattact cgatgaccaa gggcggcatc cacgcctttg cgagatcgct ggggacgcat
      661 ctggcgccgc gcggcattcg ggtgaatgtg gtggctccgg ggcccgtctg gaccccgctg
      721 aatccgtcgg acaagaacgc caaggacgtg tcgaagttcg gcgccgacac ggtgatgaaa
      781 cgtccagccc agcccgaaga aatcgcaccg gcctatgtgt ttctggcctc gccccagatg
      841 tcgagtttca tcaccggcga gattctgccg atcatcggcg gctattcagg cggctaggta
      901 ggtcgtggac gttcagacca gaatgtcttg gtagtccttg tgtcgcttga accaggcgac
      961 ggcgtagctg catcgcgggg tcagcttcag accctccgcg cgagcgtggt cggcgaggct
     1021 ctgcatgaac ttgccggcag cgccggtgcc gcgcagttcg tcatccgcct cgacatgcag
     1081 gatcgcgcgc tggctgcctt gagacgcgta gtcggcgaag acgagatgca tttcgccgtc
     1141 cgcaccggtg aagccttgtt cgaacctctg ttcggcaggc acgtcgcgaa agtcggtcat
     1201 gaaagctctt cccgggtttg ctctgggtta gaaaccggcg gggtaaggga gtgttccaag
     1261 gctgcgatga tcgccaacac tgatcaccgg cgaccgcatc gctcccatgg acaaaccgct
     1321 gtcagggcgc cctgtcgccg agcaggttca ggagaccgag gatggccttc accgtgaacc
     1381 acacatgcag gacgaacagc agcagcagag ccagcagcag gaacggaatg ccgatcagga
     1441 tcgtggacag caggctgctg atggcgacga tgacccacag cccgatggtc caccagaaca
     1501 ccgaccagaa cagcgcgatc tgggcgtcta tatgctggcg cgccaggccc gtggccgagc
     1561 ctcgcccggc gtaggcgacg atcaggccga tgatcaccag caggttggcg ctgggaatgg
     1621 acaggatgaa aagcgcccag gcgatgatcg cggcgggctt gccctcggag gtgtgaccgc
     1681 tctcaagccg cggttgttga gtgtcggtca tcctgcggtt ccttcgatca aatcaatcac
     1741 ggtcagagca accaggtttg ggggttacgc accggcttgc cggcggcaag cgtcattacg
     1801 ccccaggcgc cgcgtacgat cacccagacg gccatgagga acagcacgaa gacgccaagg
     1861 ttcaccgcaa tcaacacgac gccgacgacg ccgacgatgg cggcgatcca cagggtacgg
     1921 gtctggaatt cgaaatggct ccgcgccagc ggctggtcgg gctttgactt ggtccagacc
     1981 atgaacagcc cgatcattgc ggccaagcca aaggtcggca cggcgaacag gatcaaggcg
     2041 tagatcgtgt acaggcccat cgcaccctct ggtgcgaccg cttcggctct ggggccatcg
     2101 gctcgcccga aggcggtccg tcctgcggtc gatgagggaa ccggccgggt cgacggcggc
     2161 gtttccacgg cccaggccgg gacgggcgag ggtttcagga acgcttcatc cacccggagc
     2221 gactccctgg acggcgctgt aaacttcggc gagtggatca gggggtcggg ttccgggaac
     2281 ggtgcgacca cgggctcagg catgacgggc tgggtcgcgg ctggctcctg cgcgactggc
     2341 tcggttgcag agggcggctc aatcgcgggt ttaggcggat tgcggccggt tccggcgagc
     2401 gacgcgaaac tggtgaagcc gatcaagtca tcatgggcgt gcggatcggg ctcgggcgag
     2461 acttctgtgg tcgactctgg cgccgggcgg acaggagcag cccaggtcgg gcgctggctt
     2521 tggggcgttg cgggaggggt gtcgtcacgg aacaggtcgt cgtcgttcgg gccttgcggc
     2581 gtgccttcct ggggggaata cggatcgccg ggtttggcca tcgaagatct ccattcgggc
     2641 ggccagcgtg gccggtcgtg ctcagccgga caatgccggt cgccacggct tggcgcaacc
     2701 gtttcagggg cagcgctcgt ttcagtgtgg caggcggtgc ctgcggtcag cgggcgtagg
     2761 tcagttcgca gccgggcgcg atcgcacccg ccagggcgcc aggcttctcg acccttacgg
     2821 tgacgcgacg gacccgcacg tcctcgagac aggccagtgc aagacgttct gcgaagctct
     2881 cgaccagacc cacgtggcct tgggcgatga tgtcgcgtgc ggcctgagcg atggtttcgt
     2941 agttgatcgt gtccgaaagc ttctcgaccg gatgcggacc gagatcgagg gtgacgtcga
     3001 tcaccagcgt ctgaagccgt ccctgctcgt ggtcgtagac gccgatcccg gcctctacgc
     3061 tcaggccgcg cacgaagatc gtcagccctt cgcggcgcac ggcgtcgatg gaggggaagg
     3121 cggcgatgtc gggcaggaag gcggtcatgg attactcggc gtcgagaatg tctggagtct
     3181 gccaggccag gtgttgtccg ccatcgacgg cgatcatctg gccggtgacc atgtcggccc
     3241 cgaccagcca gagaacggcg gcggccacgg cctcggggct acccggcttt tgcaacaggg
     3301 tgccggcggc ctcggcggcg aactcggcct cggtctggtg caccgacggc agggtcgggc
     3361 cggggccgac gccgttgacg cgaatacggg gggcgagcgc ctgcgccagc gtctgcgtcg
     3421 cccaccacag gccgtttcgc gacagggcgt aggagaagaa gcgcggatcg ggcttgagca
     3481 cccgctggtc gagcagattt atgatgaccg aaccgttcgg cgcctgggct gcgaaggcct
     3541 cggccagcac caccggggcg cgcaaattgg tgtcgagatg tttgtcccag gtctcgcgcg
     3601 acaggtcgcc gacccggtcg tcctcgaaca ccgaggcatt gttgaccagc accgccaagg
     3661 ggccaagcgc ggcgacggcg gctgggatca gggccttgac ctgatcttcg tcggtcaggt
     3721 cggccttgag gattacggcg cgacgaccga gcgcgtgcac ggcgtctgct acggcctcgg
     3781 cctcttcagg ttcgctgcga tagtggatgg cgacgtcgtg gccggcctcg gccagcgcga
     3841 gacagatcgc ccgcccgatg cgtttgccgc ctccggtgac gagggcggcg cgggcctcag
     3901 tcaacccggc cgaactcgaa gtagttgatg gcgccgagaa ggacgacgaa gccgataatg
     3961 atgccaaggg ccagcatggg aatgctcctg ttcgatgcgg gtgataaagg gccagggccg
     4021 cctgtt
//
LOCUS       DASVTM010000261         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_79847, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000261 DASVTM010000000
VERSION     DASVTM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79847"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..111
                     /locus_tag="VF633_07185"
     CDS             1..111
                     /locus_tag="VF633_07185"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEX8470877.1"
                     /translation="MGTIEPLVQTVFFTVHDRIWTRMEARKAERRALAAV"
     gene            168..1715
                     /locus_tag="VF633_07190"
     CDS             168..1715
                     /locus_tag="VF633_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8470878.1"
                     /translation="MHAFETILAVLLGAVLLSLVARWLKAPFPPFLAVGGAAVAFMPF
                     APELRLDPELVLALFVAPVLLDSSFDSSPRDLKRNIRPIAFLTLATVGITVIAVAVTA
                     RWLEPQMGWAVAIALGAIVAPPDAAAAAAVLRAVPVPYRVRTILEGESLFNDGSSLLI
                     YRFAIMAAAGGMAVGWSLAATIVWALFGSVVFAIAAAWLTTRATRSITDAPSSILIQF
                     IATFGLWIAAERLGLSAIITVVVYGLTTARLAARNSATLRAPSYAVWETVVFALNAMA
                     FAMVGLQIGPVWRALEPAQHAEYAIFALAILAVAVVVRMAWVLGYNSAARLKNRVFGA
                     HLAEGESPPTVASGVIIGWAGMRGVVSLAAAYALPENFPSRDLLLLSTFAVVMGTLVI
                     QGLTLGPLIKLLGAGDDGQLPEEIKKARQLLTQAAFDHLKGLSGPIAGRLKDEYGDRL
                     ESVHADIADDGRVELEGDQIRSELLEIEREALLKLRTSGEIGEQAYYQIEEELDRHEL
                     ALIPVVR"
     gene            complement(1728..>2395)
                     /locus_tag="VF633_07195"
     CDS             complement(1728..>2395)
                     /locus_tag="VF633_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470879.1"
                     /translation="IIRSRPRAPGENQMAYIRTLIVEGHDPWLSVTPGRLPLSDADGF
                     LRRRPELRAPADAVLTRACQPLPTFNRGLRAPVGFDRAGLMQGLALLPFAWRLPALNA
                     ERREAQVAGPALYARMRLGQVLPGGGRAFARANRGIRRYADAVDPGYEVLAINLGEEP
                     SNNLSRKNRAALIGVRDGRVVWLADGDAARELGLIVALCIDDRGRTSGRRSGCTGFGH
                     YSP"
BASE COUNT          370 a          821 c          769 g          435 t
ORIGIN      
        1 atcggcacca tcgagcctct ggtccagacc gtattcttca ccgtccacga caggatctgg
       61 acccggatgg aggcccgcaa ggctgagcgc agagccctcg ccgccgtttg agtttcgcgc
      121 tctctcaagg ccgcgcgccc aaacgcgttc tggggcctta ggcggtcatg catgctttcg
      181 agacgattct ggcggtctta ctcggcgccg tcctgctgtc gctggtggcg cgatggctta
      241 aagctccctt tccgcccttc ctcgccgtcg gcggcgccgc cgtcgccttc atgccgttcg
      301 ctcccgagct gaggcttgat ccggagctgg tgctcgccct gttcgtagcg ccggttctgc
      361 tggactcgtc gttcgactct tcgccacgag acctgaagcg caacattcgg ccgatcgcct
      421 tcctgacgct ggcgaccgtc ggcatcaccg tcatcgccgt ggccgtcacc gcacgctggc
      481 tggagcctca gatgggctgg gcggtggcca tcgcactcgg cgcgatcgtg gcgccgcctg
      541 atgccgccgc agccgcagcg gtcctgcggg cggtccccgt cccctatcgc gtccgcacca
      601 tccttgaagg tgagagtctg ttcaacgacg ggagttcgct gctaatctac aggttcgcga
      661 taatggcggc ggcgggaggc atggctgtcg gttggagcct tgccgcaacc atcgtctggg
      721 ctctgttcgg cagtgtcgtc ttcgccatcg ccgccgcctg gctgaccacg cgtgcaacgc
      781 gcagcatcac cgacgcgcca tcgagcatcc tgatccagtt cattgctacc tttggcctct
      841 ggatcgccgc cgaacggtta ggcctgtcag ccataatcac cgtggtcgtc tatggcctga
      901 ccaccgcccg tcttgcagcc cgaaattctg ccaccttgcg cgccccgtcc tacgccgtgt
      961 gggagacggt ggtcttcgcc ctcaacgcca tggccttcgc catggtcggc ctgcagatcg
     1021 ggcctgtgtg gcgtgccctc gaacctgccc agcatgccga atacgcaatc ttcgccctcg
     1081 cgatcctcgc cgtggcggtg gtcgtgcgaa tggcctgggt cctcggctac aacagcgccg
     1141 caaggctcaa gaaccgggtg ttcggcgcac accttgccga aggcgagagc ccgcccaccg
     1201 tcgcgagcgg ggtcatcatc ggctgggccg gcatgcgggg cgtggtcagc ctggccgccg
     1261 cctacgccct gccagaaaac ttcccgtcca gagacctgct gctgctatcg accttcgccg
     1321 tagtgatggg gacgctggtg attcagggcc tgacgttagg cccgctgatc aagcttctgg
     1381 gtgccggcga cgacggtcag ttgcccgagg aaatcaagaa ggcgcgtcag ctgctgaccc
     1441 aggccgcctt cgatcacctg aaaggtctgt caggtccgat cgccgggcgg ctcaaggatg
     1501 aatacggcga ccggctcgag tccgttcatg ccgacattgc ggatgacggc cgggtcgagc
     1561 tggagggcga tcagatccgg tctgagctcc tcgagatcga gcgtgaggcc ttgttgaaac
     1621 ttcgaacctc cggcgagatc ggcgaacagg cctactacca gatcgaggaa gagctggacc
     1681 ggcatgaact ggccttgatc ccggtggtgc gttaggctgg cggtgcgtta gggactgtag
     1741 tgtccgaacc cggtgcagcc ggaccgacgg ccgcttgtcc gcccccgatc atcaatgcac
     1801 aaagcgacga tcaagccgag ctctctcgca gcatcgccgt cggcgagcca gaccacccgc
     1861 ccgtcccgaa ccccgatcag ggccgcccgg ttctttcgcg acaggttgtt gctgggctcc
     1921 tcccccagat tgatggccag aacctcataa cccggatcga ccgcatcggc gtagcggcgt
     1981 atgccccggt tcgcccgcgc aaaggcccgc ccaccgccgg gcaggacctg ccccagccgc
     2041 atccgggcgt acagtgccgg gccggcgacc tgcgcctcgc gccgttcggc attcagggct
     2101 ggcaaccgcc aagcgaaggg cagcagggcc aacccttgca tcaggcccgc tcggtcgaac
     2161 ccgaccggcg ctctgagccc tcggttgaac gttggcaggg gctggcaggc gcgcgtcagg
     2221 acggcatccg caggcgccct tagctccggc cgccgtcgca ggaacccgtc cgcgtcggac
     2281 aagggcagac ggccgggcgt gacagagagc cagggatcgt gcccctcgac gatgagggtg
     2341 cggatatagg ccatctggtt ctcgccgggt gcacgggggc gggaccggat tatcg
//
LOCUS       DASVTM010000262        13673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_238546,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000262 DASVTM010000000
VERSION     DASVTM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13673
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238546"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="mmsA"
                     /locus_tag="VF633_07200"
     CDS             complement(<1..1048)
                     /gene="mmsA"
                     /locus_tag="VF633_07200"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX8470880.1"
                     /translation="MREINHFIDGAAFTGASGRFADVFNPNTGDVQARVQLANDSEMD
                     RAVQSAQNAFEGWSSTNPQRRARVMFEFKQLVEANMNDLAELLSSEHGKVVADSKGDI
                     QRGLEVIEFACGIPHALKGEYTWGAGPGIDVYSMRQPLGVVSGITPFNFPAMIPMWMF
                     GVAIAVGNTFILKPSERDPSVPVRLAELMMEAGAPAGVLNVVHGDKQAVDAILTHPLV
                     HAVSFVGSSDIAHYVYQTGAANHKRVQAMGGAKNHGIVLPDADMDQVIKDLSGAAYGS
                     AGERCMALPVVVPVGKKTADELRERMVAEIPTMRVGVSTDAGAHYGPVVTAQHKARVE
                     GWIDQGVKDGAELVV"
     gene            1122..1760
                     /locus_tag="VF633_07205"
     CDS             1122..1760
                     /locus_tag="VF633_07205"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8470881.1"
                     /translation="MSSPSTVISATADDLWSAPGRPRDPDAKREAILVAAATAFVRDG
                     YGATSIDDVAARLGVSKPTVYYYAGSKQALLSACFMIALDGYSEAVEESAVSGADGRE
                     RLHQVVQKYVEITTTVFGRCLHRVPDVELTPPVRERLRAVKRKVDARIRDLLREGVRD
                     GSLRTADVRMSTFAISGAMNSIAQWYAPDGKLSPEEIAERLWDAFSEGVRPR"
     gene            1823..3355
                     /locus_tag="VF633_07210"
     CDS             1823..3355
                     /locus_tag="VF633_07210"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX8470882.1"
                     /translation="MQSILEQLEQRRAAARLGGGAARIAAQHAKGKLSARERVDVLLD
                     EGSFEETDMFVEHRSTQFGMDKQRIPGDGVVTGRGTIGGRLVYVFSKDFTVFGGSLSG
                     AHAAKIVKLQKMALTTGAPIIGLFDAGGARIQEGVEALAGYADIFLQNTLASGVIPQI
                     SVIMGPCAGGDVYSPAITDFIFMVKDTSYMYVTGPDVVKTVTNEVVTHEDLGGAKVHA
                     QKSGVADGAFENDLEALSEVRRLIGFLPLSNREKPPVRESYDEPDRDEASLDTLIPAN
                     PNQPYDMKELILKTVDEADFFEIGADFAKNIICGFGRLDGQPVGIVANQPQVLAGVLD
                     IDSSRKAARFVRFCDAFGIPLLTLVDVPGFMPGTKQEYGALIKHGAKLLFAYAEATVP
                     KLTLITRKAYGGAYDVMSSKHLRGDVNYAWPTAEIAVMGAKGAVEIIFRKEAGDPEAL
                     AAREAEYKDKFANPFVAAGLGYIDDVIMPHGTRRRLIKALKTLENKTQENPWKKHDNI
                     PL"
     gene            3365..4489
                     /locus_tag="VF633_07215"
     CDS             3365..4489
                     /locus_tag="VF633_07215"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061810 - NAD glycohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8470883.1"
                     /translation="MSGQQFIALADELVRAQSAYAVLASPSGPVGALAKEAEKMNLAW
                     SQSSIGPHANVYYEGLLEAPAGYHFDSEWGLSGRGSFSRTRGNWASFTAAEIKAEVAR
                     RAGQPNLDPVEKAAQGVRWRLEQIKAEALSMIAIEIAERSDSYLSSLRDQIAEVSPRS
                     VNDFQELMVPKRQFMTRDTIAAAAGLKIAPHQEILAQVASLREVSEVTNQLEKLLRQA
                     GSHLLRLESRGRQQSLVGTNVFIGHGRSAMWRELKDFVGERLHLPYDEFNRVPVAGVT
                     NIARLSEMLGAAAVALIVLTAEDEQADGAMHARQNVIHEAGLFQGKLGFTKAIVLLEE
                     GCAEFSNIQGLGQIRFPTGKISAAFEEVRRVLEREGLIEP"
     gene            4578..6572
                     /locus_tag="VF633_07220"
     CDS             4578..6572
                     /locus_tag="VF633_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX8470884.1"
                     /translation="MFSKILIANRGEIAVRIIKTARKMGIQTVLVHSDADTGSLAYEM
                     ADETIHIGPSPVAQSYLIQDKIVDAVRKSGAEAVHPGFGFLSENPVFARRLEAEGITF
                     IGPNPYAIEAMGDKITSKKFAAEAGVTTVPGYMGLIESPEHAVQIAGEIGYPVMIKAS
                     AGGGGKGIRVAHSDDDMAEGFAAVKAEALTAFGDDRVFLEKFIVNPRHIEIQVLGDKH
                     GNIVSLFDRECSIQRRNQKVIEEAPSPLLDDATRKLMGDQAIALARAVNYDSAGTVEF
                     VAGQDRSFYFLEMNTRLQVEHPVTELITGVDLVEQMIRSAAGETMAFGQDDLKINGSA
                     IESRIYAEDPYRGFLPSIGRLVRYEQAEEGEHESPIGTYVVRNDSGVREGDEISMFYD
                     PMISKLCAWAPTRAGAVEGMARALEDTHLQGVGHNVPFLAAVMDQERFKSGHLSTSYI
                     VDEFPDGFNGVPAGPLQKDVMVAAAVAMNEILADQSGDPSPRTDWIVLIDREPIGVSV
                     GYDDEEALTLDLIGEQRALRLSEIDWRPGLAQFRGVLDDAPFTAEVKRIADGFEIRSR
                     ASKSRVRVMSPHHAELFARLPEKVAADTSKLIQSPMPGLVVSIAVVPGQSVKTGETVA
                     IIEAMKMQNIMKAERDGVVKSVGAKAGDPVAADDVLVEFE"
     gene            6572..7981
                     /locus_tag="VF633_07225"
     CDS             6572..7981
                     /locus_tag="VF633_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8470885.1"
                     /translation="MSGVSVFRVPTPEQWRESAIKALKDKPLESLLHLDADHLAIRPL
                     YADASGITPLFTPRPTDAEGRSWDLRTLVEGEDPDTVNAAVLTDLEGGAASVILSAPS
                     FAKSVLSDSEPLGRALANVALELAAVGLDAGLNGPDAANALAVVAKGSPRAKLMFHMD
                     PISAFALAGGSPLPIALHLSQSAAAAERHAGAYPDATFFLASGRVVHEAGGSAGQELG
                     FLAATVVAYVRAAVDAGLSAEQALKGTVLGVAVDAEYFDSLAKVRALRLIWASVSKAF
                     GLDVPANIEARSSRRMLAARDPWPNLLRLTAAGFAGAVGGADSVVLDGFSRASGRPDA
                     FARRQARNTQIVLMEEAHLGRVDDPAAGSWFLDSRTHDLATAGWAEFQRIEREGGIVA
                     ALKGGAIQMRVAASRASLKAAFDNGSKQIIGVTKFVDPDVRAAPVEASSPPAKAAITD
                     ACIALTSIRFAAAFEEASQ"
     gene            7978..10137
                     /gene="scpA"
                     /locus_tag="VF633_07230"
     CDS             7978..10137
                     /gene="scpA"
                     /locus_tag="VF633_07230"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX8470886.1"
                     /translation="MSFPDFTKATLDFGATGTGPAREPWTTPEGIEVASAYDASAIAG
                     NESVHGLPGFAPFLRGPYPTMYAGNPWTIRQYAGFSTAEASNAFYRRNLAAGQKGLSI
                     AFDLATHRGYDSDHPRVKGDVGMAGVAIDSILDMRTLFDGIPLDEMSVSMTMNGAVLP
                     ILALYIVAGEEQGVPHAKLTGTIQNDILKEFMVRNTYIYPPEPSMRIIADIFEWTAKE
                     TPKFNSISISGYHMQEAGASADIELAYTLSDGIEYLRAGKAAGMEIDRFAPRLSFFWA
                     IGMNYFMEVAKMRAGRLLWAEAVQKEGGVDPKSLSLRAHCQTSGWSLAAQDVFNNVSR
                     TMVEAMAAAGGQTQSLHTNALDEALALPTDFSARIARNTQILLQQETGLTRVIDPWGG
                     SYYVEKLTQELADKARALMAEVEAAGGMAKAIEAGIPKLRIEESAAKTQARIDTGKQT
                     VVGVNRYLNDVADDIPMLKVDNAAVLAAQIEKLKRLRAERDETATQAALAALTEGAKG
                     TANLLELSVQAARAHASVGEISDALEAAFGRHVATVKTVSGVYAREAGKDARFARARD
                     MVATFVDNDGGPPRILIAKLGQDGHDRGQKVIATGYGDLGLDVTAGALFQTPAEAARD
                     AVNANVHAVGASSLAAGHLTLVPELRAELEKLGRPDIMIVVGGVIPPSDVQTLIDMGA
                     AAVYPPGSTVADTAIDLIEKLNQRLGYAQPAPSREPA"
     gene            10134..10556
                     /gene="mce"
                     /locus_tag="VF633_07235"
     CDS             10134..10556
                     /gene="mce"
                     /locus_tag="VF633_07235"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX8470887.1"
                     /translation="MIGKLNHVGVATPSIADSIVLYRDVLGATKIGEPLDLPAQGVKV
                     CFVDLPNAQIELIEPFDDTSPIVGFLAKNPTGGQHHVCFEVEDIHAAVAEAYAKGLTI
                     LGTGEPRIGAHGTPVVFLHPKQMGGVLIELMETPPSDH"
     gene            10614..12971
                     /locus_tag="VF633_07240"
     CDS             10614..12971
                     /locus_tag="VF633_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470888.1"
                     /translation="MTSASLAHASALALVVSGVLAAPVLAQTTQIDEIVVTAERRETS
                     LRRTPLSVSVAAQPLLAGAGVVDIKDLAILTPGLLVASSSNQTNTTARIRGIGTVGEN
                     PGLESSVGVIVDGVFRARNGVALGDLGEVERTEVLRGPQSTLFGQNTSAGVINIVTAG
                     PKFDFGATGEATVGDHGARGGSVSVTGPLIEDKLAGRLYAAVRRRDGFYDVRTGNGPR
                     TETDDQNEAYETVRGQLLWTPTASISNRLSADFTHRDERCCIGVQQSTGPTAGILALL
                     SPDGGVMNPAALSARTGYANRDTNTRIKDGGVSLHTKIDTRLGVVELTTAARQWRAVL
                     SQDWDFTSADIAYRPDDGRWSNRFRTFSHEARLTGSRGAFDYTGGVYLMRETLLRRDA
                     FLYGSAYEDYLGRILTRSAANPVGLAGYVSTLTRLPVGQNFVSGQGALDTYKQTATTA
                     ALFGQTTWHATDKLGLTLGLRYSHIEKDLDAAYTNTDSGRACAAALARGITNATICLP
                     WANPAFNNLSRSEGQTDDGLSGLARVEYQLTPSVLTYVSYARGRKSAGYNLDRAQTNL
                     IPDASTAFAPEVVDSVEVGAKATLLGGRLLLSGDAFHQAYTDFQLNTFLGTTFLVKSI
                     PEVTAKGAELEGTLRPMPGLSLQAGVVYAQTEYGDAPVPGLPLLAGSRLSFAPLWSGS
                     LSGTYRRDIGDGFAVRATLAGKYSSSYNTGSDLAPEKVQPGFWLANGSVGLEAEAGWS
                     VELWGRNLFDEDYRQVAFGAPFQAGTIGAFLGAPRTVGVTLRVTR"
     gene            complement(12996..>13673)
                     /locus_tag="VF633_07245"
     CDS             complement(12996..>13673)
                     /locus_tag="VF633_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX8470889.1"
                     /translation="GLVCCLVLGPRYGFGRDNMAPANLAYTAIGTGLLLVGWIGFNAG
                     SAGAADALAGTACFNTLLAAAAASLGWLAIEWIEKKKPTLLGLLSGIVAGLVAITPAA
                     GFVDPKGAFFIGVIAGAACYAGAVWVKHALKYDDSLDAWGIHGIGGIVGALLTGIFAT
                     TAVNPLSEGASVLKQAIGLAGVIAWSAIGTLAVLMICKFTVGLRVTKEGEVEGLDYSE
                     HGESIHS"
BASE COUNT         2457 a         4608 c         4305 g         2303 t
ORIGIN      
        1 cgacgaccag ctcggcgccg tccttgaccc cctgatcgat ccagccctcg acgcgagcct
       61 tgtgctgcgc cgtaacgacg ggaccatagt gggcgccggc gtcggtcgag acgccgaccc
      121 gcatcgtcgg gatctccgcg accatccgct cacgcagctc atccgccgtc ttcttgccga
      181 ccggcaccac gaccggcagc gccatgcagc gctctccggc cgagccatag gccgcgcctg
      241 acaggtcctt gatcacctga tccatgtcgg cgtcaggcag gacgatgccg tggttcttgg
      301 ccccgcccat ggcctgaacc cgcttatggt tcgcggcgcc ggtctgatag acatagtgcg
      361 cgatatcgga cgaaccgacg aagctgacgg catgcaccag cgggtgggtc aggatggcgt
      421 cgacggcctg tttgtcgccg tgcacgacgt tcagcacacc ggcgggagcg cccgcctcca
      481 tcatcagttc ggccaaccgc accggcaccg aagggtcgcg ctcggacggc ttgaggatga
      541 aggtgttgcc gaccgcgatg gccacgccga acatccacat cgggatcatg gccgggaagt
      601 tgaacggcgt gatgcccgac acgacgccca gcggctggcg catcgaatag acgtcgatcc
      661 cgggacctgc gccccaggtg tattcgccct tcagcgcatg cgggatgcca caggcgaact
      721 cgatgacctc gaggccgcgc tggatgtcgc ctttggagtc ggcgacgacc ttgccgtgct
      781 cggacgacag cagctcggcg aggtcgttca tgttggcctc gaccaactgt ttgaactcga
      841 acatcacccg ggcccggcgc tgcgggttgg tcgaggacca gccctcaaac gcgttctggg
      901 cggactgcac cgcgcggtcc atctcggagt cattcgccaa ctggacccgc gcctgaacgt
      961 cgccggtgtt ggggttgaag acgtcggcga accgaccgga tgcaccggtg aaggccgccc
     1021 cgtcgatgaa gtggttgatc tcgcgcatgc tgcgttccct tgtcccggtg tcccggatca
     1081 tcagtggctg acaatcgggg cttctgtgct atttctgacc catgagtagc ccatcgaccg
     1141 tgatctccgc aaccgcagac gacctgtggt ctgcaccggg tcgtccgcgc gatcccgacg
     1201 ccaagcgcga ggctattctg gtcgccgccg ccacggcctt tgtgcgtgac ggctatggcg
     1261 ccacctcgat cgatgacgtt gcggcccggc tcggcgtgtc caagccgacg gtctattact
     1321 acgccggatc gaaacaggcc ctgctcagcg cctgtttcat gatcgcgctc gatggctatt
     1381 cggaagcggt cgaggaaagc gcggtctcgg gcgccgacgg tcgcgaacgt ctgcatcaag
     1441 tcgttcagaa atatgtagaa atcaccacga cggtattcgg tcgctgcctt caccgggtgc
     1501 ctgatgtcga gctgacgccg ccggtgcgcg agcgtctgcg ggcggtcaag cgcaaggtgg
     1561 acgcccgtat ccgcgacctg ctgcgagagg gcgttcgtga cggatcgctg cgcacagccg
     1621 acgtccgcat gagcaccttc gccatctcgg gcgccatgaa ctcgatcgcc cagtggtacg
     1681 cgccggacgg caagctcagc cctgaggaaa tcgccgaacg tctgtgggac gccttttccg
     1741 aaggggtccg cccgagatga gccagacatt gacgcacacc acgccttccg ccaataactc
     1801 tactcgcaag taggaatacg agatgcaatc catcctcgaa cagctggaac agcgccgtgc
     1861 agccgcccgc ctcggcggcg gtgcagcgcg gatcgcggcc cagcacgcca agggcaagct
     1921 gtcggcccgc gaacgcgtcg atgtgctgct ggacgaaggc tcgttcgagg agaccgacat
     1981 gttcgtcgag catcgctcga cccagttcgg catggacaag cagcgcatcc ccggcgacgg
     2041 cgtggtcacc gggcgcggca ccatcggcgg tcgtctggtc tatgtcttct ccaaggactt
     2101 caccgtcttc ggcggttcgc tgtcgggcgc tcatgcggcc aagatcgtca agctgcagaa
     2161 gatggcgctg acgaccggcg caccgatcat cggcctgttc gacgccggcg gggcccgaat
     2221 ccaggagggc gtcgaggccc tggccggtta cgccgacatc ttcctgcaga acacccttgc
     2281 ctcgggtgtc atcccccaga tcagcgtcat catggggccg tgcgcgggcg gcgacgtcta
     2341 ttcgcccgcc atcaccgact tcatcttcat ggtgaaggac acgtcctaca tgtacgtgac
     2401 cggccccgac gtggtgaaga ctgtcaccaa cgaggtcgtg acgcacgagg atctgggcgg
     2461 cgccaaggtc catgcccaga aatccggcgt cgccgacggc gcgttcgaga acgatctgga
     2521 ggccctgtca gaggtccgcc gcctgatcgg tttcctgccg ctgtcgaacc gcgagaagcc
     2581 tccggtgcgc gaaagctacg acgagcccga ccgcgacgag gcctctctcg acaccctgat
     2641 cccggccaat ccgaaccagc cctacgacat gaaggagctg atcctgaaga cggtcgacga
     2701 ggccgacttc ttcgagatcg gggccgattt cgcgaagaac atcatctgcg gcttcgggcg
     2761 tctggacggc cagccggtcg gcatcgtcgc caaccagcct caggtgctgg cgggtgttct
     2821 ggacatcgat tcatcgcgca aggcggcccg gttcgtgcgt ttctgcgatg ccttcggcat
     2881 cccgctgctg acgctggtgg atgttcccgg cttcatgccc ggcaccaagc aggagtacgg
     2941 cgccctgatc aaacacgggg ccaagctgct gttcgcctat gccgaggcga cggtgcccaa
     3001 actgaccctg atcacgcgca aggcctatgg cggcgcctat gacgtaatga gctccaaaca
     3061 cctgcgcggc gacgtcaact acgcctggcc gaccgccgag atcgcggtca tgggggccaa
     3121 gggcgcggtc gagatcatct tccgcaaaga ggccggcgac cccgaggcgc tggccgcccg
     3181 cgaggccgag tacaaggaca agttcgccaa ccccttcgtc gccgctggcc tcggctacat
     3241 cgacgacgtc atcatgccgc atggcaccag gcggcggttg atcaaggcgc tcaagacgct
     3301 cgagaacaaa acccaggaaa atccctggaa gaagcacgac aacattccgc tgtaggtgac
     3361 agtcatgtcc ggccaacagt tcattgccct agccgatgag ctagttcggg cgcagtcagc
     3421 gtatgctgtc ttggcgtcgc cgtccggccc agttggcgcg ctggccaagg aagctgagaa
     3481 gatgaatctg gcttggagtc agtcatctat cggtccccat gcgaacgtgt actacgaggg
     3541 attgcttgag gcgcccgcag gctatcattt cgactccgaa tggggactta gtgggcgagg
     3601 aagtttcagc cgaacgcgcg gaaactgggc atcattcacc gctgctgaaa taaaggctga
     3661 agttgcgcgt agggcggggc agcccaactt ggatcccgtc gaaaaagcag cgcaaggagt
     3721 tcgttggagg cttgagcaga tcaaggcaga agctctgtct atgattgcga tcgagatcgc
     3781 agagcgaagc gatagctact taagctcatt gagggatcaa atcgcagagg tcagccctcg
     3841 atccgtcaat gattttcaag agctcatggt gccaaaacgg cagttcatga cacgtgacac
     3901 gatcgccgca gccgccggtc tgaaaatcgc cccccaccaa gaaattctag ctcaggtcgc
     3961 ttctctccgg gaagtctcgg aagtcacaaa tcagcttgaa aaactgttgc gacaggcggg
     4021 atctcatctt cttcgccttg agtcgcgagg gcggcagcag tcactagtag gaacgaatgt
     4081 gttcatcggc catggccggt ccgccatgtg gagagagttg aaggatttcg tcggggaaag
     4141 gcttcatctt ccgtacgatg aattcaaccg ggtgcccgtc gcgggtgtta ccaacatagc
     4201 gcgattgtcg gagatgctag gcgcggcggc ggttgcactg attgttctga ctgccgaaga
     4261 tgaacaggca gatggcgcga tgcacgccag acagaacgtc atccatgagg cgggtctctt
     4321 tcaaggcaag ctcggattca cgaaggcgat agtgcttctt gaagagggct gcgctgagtt
     4381 ctctaatatc caaggcttag gccaaattcg gtttcccact gggaaaataa gtgccgcttt
     4441 tgaagaggtt cgtcgtgtgc tggagcgcga aggcctgatc gagccatgaa ctctacctgc
     4501 tcatatttcc cctacctcag cttcatgagg acgccatcgc ggccgaggcg gcccggcaca
     4561 aaattcagga cctagtgatg ttctctaaaa tcctcatcgc caaccggggc gagatcgctg
     4621 ttcggatcat caagaccgcg cgcaagatgg gcatccagac ggtgctggtg cactcggacg
     4681 ccgacaccgg atcgctggcc tatgagatgg ccgacgagac gatccacatc ggtccgtcgc
     4741 ccgtagcgca gtcctatctg atccaggaca agatcgtcga cgccgtgcgg aaatcgggcg
     4801 ccgaggccgt gcatccgggc tttggcttcc tgtcggaaaa ccccgtcttc gcgcgtcgcc
     4861 tcgaggccga gggcatcacc ttcatcgggc ccaatccata tgcgatcgag gcgatgggcg
     4921 acaagatcac gtccaagaag ttcgcagccg aggcgggcgt caccaccgtg cccggctata
     4981 tggggctgat cgaaagcccc gaacacgccg tccagatcgc cggagaaatc ggctatccgg
     5041 tgatgatcaa ggcgtcggcg ggcggcggcg gcaagggcat ccgcgtcgcc cattccgacg
     5101 acgacatggc cgagggtttc gccgccgtga aggccgaggc cctgaccgca ttcggcgacg
     5161 accgggtgtt cctcgagaaa ttcatcgtca acccgcgcca catcgagatc caggtgctgg
     5221 gcgataaaca cggcaatatc gtcagcctgt tcgaccgcga atgctcgatc cagcgtcgca
     5281 accagaaggt catcgaggag gcgccgtcgc ccctgctcga cgacgctacg cgcaagctga
     5341 tgggcgatca ggccatcgcc ctggcccgcg ccgtgaacta cgactccgcc ggcacggtcg
     5401 agttcgtcgc cggtcaggac cgcagcttct acttcctcga aatgaacacc cggctgcagg
     5461 tcgaacaccc ggtgaccgag ctgatcaccg gcgtcgatct ggtcgagcag atgatccgct
     5521 cggcggcggg cgagaccatg gccttcggtc aggacgacct gaagatcaac ggctcggcga
     5581 tcgaaagccg catctatgcc gaagacccct atcgcggctt cctgccctcc atcggccgcc
     5641 tcgtccgtta cgaacaggcg gaggagggcg agcatgaatc gcccatcggg acctatgtgg
     5701 tgaggaacga ttccggcgtc cgcgagggcg acgaaatcag catgttctat gacccgatga
     5761 tctcgaaact gtgcgcctgg gccccgaccc gcgcgggcgc cgtcgagggc atggcgaggg
     5821 ctctggaaga cacccacctg cagggcgtcg gccacaacgt gcccttcctt gccgccgtca
     5881 tggatcagga acggttcaag tcgggccatc tctcgaccag ctatatcgtc gacgaattcc
     5941 ccgacggctt caacggcgtc ccggctggtc cgctgcagaa ggacgtcatg gtcgccgccg
     6001 ccgtggccat gaacgaaatc ctcgccgacc agtccggcga cccgtcgccg cgcaccgact
     6061 ggatcgtgct gatcgaccgc gagccgatcg gcgtctcggt cggttacgac gacgaggaag
     6121 cgttgacgct ggacctgatc ggcgagcagc gggcgttgcg tttgtccgag atcgactggc
     6181 gtccggggct ggcgcagttc cgtggcgtgt tggatgacgc gccgttcacc gccgaggtca
     6241 aacgcatcgc cgacggcttc gagatccgtt cgcgcgcctc gaaatcccgc gtccgggtga
     6301 tgtcgcccca ccacgccgaa ctgttcgctc gcctgcccga aaaggtcgcc gccgacacct
     6361 cgaaactgat ccagtcgccg atgcccggcc tcgtcgtctc catcgccgtc gtccccggac
     6421 agtccgtgaa gaccggcgaa accgtcgcca tcatcgaagc catgaagatg cagaacatca
     6481 tgaaggccga gcgcgacggc gtcgtaaaat ccgtcggcgc caaggccggt gaccccgtcg
     6541 ccgccgacga cgtgctggtc gagttcgagt gatgtcaggc gtctccgttt ttcgcgttcc
     6601 gacgccggag caatggcgtg agtctgcgat caaggcgctg aaggacaagc cgctcgagtc
     6661 gctgctgcat ctggacgccg atcatctggc gatccgtccc ctgtacgccg acgccagcgg
     6721 catcacgccg ctgttcacgc cgcgcccgac cgatgccgag ggccggtcgt gggacctgcg
     6781 gacactggtc gagggcgaag atccggacac ggtcaatgcg gcggtgctga ccgatctcga
     6841 aggtggcgcg gccagcgtca tcctgtcggc gccctctttt gccaagtcgg tgctttcgga
     6901 ctccgaaccg ctgggccggg cgttggcgaa cgtggcgctg gaactggccg ccgtgggtct
     6961 cgacgccggg ctgaacggcc ccgacgccgc caacgctctg gccgtcgtgg ccaagggctc
     7021 gccgcgcgcg aagctgatgt tccacatgga cccgatctcg gcgttcgccc tcgcaggcgg
     7081 ttcgcccctg ccgatcgcgc ttcatctcag ccagtccgcc gccgccgccg aacgccacgc
     7141 aggcgcctac cccgacgcga ccttcttcct cgcctcgggc cgcgtggtgc acgaggccgg
     7201 tggatccgcc gggcaggagc tcggctttct cgccgccacc gtggtcgcct atgtcagggc
     7261 tgccgtggac gccggattgt ctgcggaaca agcgctcaag ggcacggtcc tcggcgtcgc
     7321 ggtcgacgcc gaatattttg acagtctcgc taaagtccgc gcgctgcgcc tcatctgggc
     7381 gtcggtcagc aaggccttcg ggctcgacgt ccccgccaat atcgaagcgc gatcgtcgcg
     7441 ccgaatgctg gccgcccgcg acccctggcc caacctgctc aggctcaccg ccgccggttt
     7501 cgcaggcgca gtcggcggag cagactcggt cgtgctggac ggcttcagcc gcgcctcggg
     7561 ccgccccgac gccttcgccc ggcgtcaggc gcgcaacacc cagatcgtcc tgatggaaga
     7621 ggcgcacctc ggacgggtcg atgatcccgc ggcgggatcg tggttcctcg acagccgaac
     7681 ccacgatctt gcgaccgccg gctgggccga gtttcagcgc atcgaacgcg agggcggcat
     7741 cgtcgccgct ttgaaaggcg gcgcgatcca gatgcgcgtc gccgcctcac gcgcctccct
     7801 gaaagccgcc ttcgacaacg gctccaaaca gatcatcggc gtaaccaaat tcgtcgatcc
     7861 agacgtgcgg gcggcgccgg tcgaggcctc atcaccgcca gccaaggccg ccatcaccga
     7921 cgcgtgcatc gccctgacgt ccattcgctt cgccgccgcc ttcgaggagg ccagccaatg
     7981 agtttccccg acttcaccaa ggccacgctc gattttggcg ccaccggcac aggccctgcg
     8041 cgcgagccgt ggaccacgcc cgaaggcatc gaggtcgcct cggcctatga cgcctcggcc
     8101 atcgcgggca atgaatccgt acacggacta cccggattcg caccgttcct gcgcggcccc
     8161 tatccgacca tgtacgcggg caatccgtgg accatccgcc agtacgcggg cttctcgacc
     8221 gccgaggcct cgaacgcctt ctatcgtcgc aacctcgcgg ccggtcagaa ggggctcagc
     8281 atcgccttcg atctggccac gcaccggggc tatgattccg accacccccg ggtgaagggc
     8341 gacgtcggca tggccggtgt ggcgatcgat tccatcctcg acatgcgcac cctgttcgac
     8401 ggcattccgc tcgacgagat gtcggtgtcg atgaccatga acggcgccgt gttaccgatc
     8461 ctggcgctct acatcgtggc gggcgaggag cagggcgtgc cgcacgccaa actgaccggg
     8521 accatccaga acgacatcct caaggagttc atggtccgca acacctacat ctatccgccc
     8581 gagccctcga tgcggatcat cgccgatatc ttcgagtgga cggcgaagga aacgcccaag
     8641 ttcaactcga tctcgatcag cggctatcac atgcaggagg cgggagcctc ggccgacatc
     8701 gagctggcct acaccctgtc ggacgggatc gaataccttc gcgcgggcaa ggccgcgggc
     8761 atggagatcg accggttcgc gccgcgcctg tcgttcttct gggccatcgg catgaactac
     8821 ttcatggagg tcgccaagat gcgcgccggc cgcctgttgt gggcagaggc agtgcaaaag
     8881 gaaggcggcg tcgatcccaa gtcgctgtct ttacgcgccc actgccagac ctccggctgg
     8941 tcgctggcgg cgcaggatgt gttcaacaac gtttcgcgca ccatggtcga ggccatggcg
     9001 gcggcgggtg gccagaccca gtcgctgcac accaatgcgc tggacgaggc gctggccttg
     9061 cccaccgatt tctcggcgcg gatcgcgcgc aacacccaga ttctgttgca gcaggagacc
     9121 ggcctgaccc gcgtcatcga cccgtggggc ggcagctact acgtcgagaa actgacccag
     9181 gaactggccg acaaggcccg cgccctgatg gccgaggtcg aggccgcagg cggtatggcc
     9241 aaggccatcg aggccgggat tcccaagctg cggatagagg agtcggcggc gaaaactcag
     9301 gcccggatcg atactggcaa acagaccgtc gtcggtgtga accgctatct caacgacgtc
     9361 gccgacgaca ttccgatgct caaggtcgac aacgccgccg tcctcgccgc gcagatcgag
     9421 aagctgaaac gcctccgcgc cgaacgcgat gagacggcca cccaagccgc gctggccgcc
     9481 ctgaccgagg gcgcgaaggg gaccgcgaac ctgctggaac tgtcggtaca ggctgctcgc
     9541 gcgcatgcct cggtcggcga aatctccgat gctctggaag ccgccttcgg tcgtcacgtc
     9601 gccacggtga agaccgtctc cggcgtctat gcccgggaag ccggtaagga cgcacggttc
     9661 gcacgggccc gcgatatggt ggctacgttt gtcgataatg acggtggccc gccccgcatc
     9721 ctgatcgcga aattgggtca ggacggccac gatcgcggcc agaaggtcat cgccaccggc
     9781 tatggcgacc tcggtctcga cgtcaccgca ggggccctgt tccagacacc cgccgaagct
     9841 gcgcgcgacg cggtcaacgc caatgtccac gcggtcggcg cctcgtcgct ggcggccggg
     9901 catctgacgc tcgtgccgga acttcgcgcc gagctggaga agctgggccg accagacatc
     9961 atgatcgtgg tgggcggagt gatcccgccg tcggacgtcc agaccctgat cgatatgggc
    10021 gccgccgccg tttatccgcc cggctcgacc gtcgccgaca ccgccatcga cctgatcgag
    10081 aagctcaacc agcgcctcgg ctacgcccag cccgccccat cgagagagcc cgcatgatcg
    10141 gcaagctgaa ccacgtcggc gtcgccacgc cgtcgatcgc agactccatc gtgctgtacc
    10201 gtgacgtgct gggcgccacg aagatcggcg aaccgttgga cctgccggcg cagggcgtga
    10261 aggtctgttt cgtcgacctg ccgaacgccc agattgagct catcgagccg ttcgacgaca
    10321 ccagccccat cgtcggcttc ctcgccaaaa accccacggg tggtcagcat cacgtctgtt
    10381 tcgaggtcga ggatatccac gccgccgtcg ccgaagccta cgccaagggc ctgaccattc
    10441 tgggcacagg cgagccccgc atcggcgccc acgggacccc cgtcgtcttt ctgcatccca
    10501 aacagatggg cggcgtcctg atcgaactga tggaaacgcc gccctccgac cactgagacg
    10561 gccgcacacc gacctctcgt ccgacgtcct ctcctccccc aaagagacac tgtatgacct
    10621 ccgcctccct cgcccacgcg agcgccctcg cgctcgtcgt ctccggcgtg ctcgctgctc
    10681 ccgtcctcgc ccagacgacc cagatcgacg aaatcgtcgt caccgcagag cgtcgcgaaa
    10741 cctcgcttcg gcgcacgccg ctgtcggtgt cggtcgcagc ccagccgctg ctagcgggcg
    10801 cgggtgtcgt cgacattaag gacctagcta tcctgacgcc gggcctgctg gtcgcgtcgt
    10861 cttcgaacca gaccaatacc acggcgcgga tacggggcat cggcacggtc ggtgagaacc
    10921 cggggctgga aagctcggtc ggcgtcatcg tcgacggtgt gttccgcgcc cgtaacggcg
    10981 tcgctctcgg cgatctcggc gaggtcgaac gcaccgaggt cctgcgcggc ccgcaatcga
    11041 ccctgttcgg tcagaacacc tccgccgggg taatcaacat cgtcaccgcc gggccaaagt
    11101 tcgactttgg cgccacaggc gaggccacgg tcggcgacca cggcgctcgc ggcggctcgg
    11161 tttcggtgac cggccccctg atcgaggaca agctggcagg ccgtctttac gcagccgttc
    11221 gtcgccgaga cggcttctat gacgtgcgca cgggcaatgg ccctcgcacc gaaaccgacg
    11281 accagaacga ggcctacgag accgttcggg gccaattgct gtggacgccg accgccagca
    11341 tcagcaaccg cctgagcgcc gacttcaccc atcgtgacga gcgctgctgc atcggcgtgc
    11401 agcagtccac cgggccgacc gcaggcatcc tcgccctgct gagtcccgac ggcggcgtga
    11461 tgaacccggc cgctctctcg gctcgcaccg gctacgccaa ccgcgacacc aacactcgca
    11521 tcaaggacgg cggcgtctcg ctgcatacca agatcgatac gaggctgggc gtggtcgaac
    11581 tgaccaccgc cgcccgccag tggcgcgctg tgctcagcca ggactgggac ttcacctccg
    11641 ccgacatcgc ctatcgtccc gatgacggtc gctggtccaa ccggttccgc accttcagcc
    11701 atgaggcccg gctgaccggc tcgcgcggcg ctttcgacta caccggcggc gtctatctga
    11761 tgcgcgagac cctgctgcgg cgggacgcct tcctctacgg ctcggcctac gaagactatc
    11821 tcggccgcat cctgacccgt tccgccgcca acccggtcgg cctcgcggga tacgtctcga
    11881 ccctgaccag attgcccgtg ggccagaact tcgtgtcggg gcagggggcg ctcgatacct
    11941 acaaacagac cgccacgacc gccgcgctgt tcggccagac gacctggcat gcgaccgaca
    12001 agctcgggct gacgctcggc ctgcgctaca gccatatcga gaaggatctc gacgcggcct
    12061 acaccaacac cgacagcggt cgggcctgcg ccgcagccct cgcgcgaggc atcaccaatg
    12121 ccacgatctg cctaccctgg gccaacccgg ccttcaacaa tctgagccgc agcgagggcc
    12181 agacggacga tggattgtcg gggctggcgc gcgtcgaata ccagctgact ccgtctgtgc
    12241 tgacctatgt ttcctatgcc cggggccgca aaagtgcggg ctacaacctc gaccgcgccc
    12301 agaccaacct gatccccgac gcctcaaccg cctttgcgcc cgaggtggtc gattccgtcg
    12361 aggtcggcgc caaggcgacg ttgctgggcg ggcggctgct gctgtcgggc gacgcctttc
    12421 atcaggccta taccgacttc cagctcaaca ccttcctcgg gacgaccttc ctcgtgaagt
    12481 cgatccccga ggtgaccgcc aagggcgccg aactggaggg gacgctgcgc ccgatgccgg
    12541 gcctcagcct tcaggctggc gtagtctatg cccagacgga atacggcgat gcgcccgtgc
    12601 cgggcctgcc gctgctggcc ggttcgcggc tgtcgttcgc gccgctctgg tcgggatcgc
    12661 tttctggcac gtaccgccgc gatatcggcg acggtttcgc ggtgcgcgcc actctcgcag
    12721 gcaaatattc gtcgtcctac aacaccggtt cggatctggc gcccgagaag gtgcagccgg
    12781 gcttctggct ggccaacggc tcggtcggcc ttgaagccga agccggttgg tcagtcgagc
    12841 tctggggccg caacctgttc gacgaggact atcgtcaggt cgcgttcggg gcgccgttcc
    12901 aggcgggcac catcggcgcc ttcctcggcg caccgaggac ggtcggggtg acgctcaggg
    12961 tcacgcgctg accctgagcg ttctggaacc ggcgatcagg agtggatgct ctcgccgtgt
    13021 tcggaatagt cgaggccctc gacctcgcct tccttggtga cgcgcaggcc gacggtgaat
    13081 ttgcagatca tcagcacggc caaggtgccg atcgcgctcc atgcgatcac gccggcaagg
    13141 ccgatcgcct gtttcagcac cgaggcgcct tccgacagcg gattgacggc ggtggtggcg
    13201 aagatgccgg tcagcagggc gccgacgatg ccgccgatgc cgtggatgcc ccaggcgtcg
    13261 aggctgtcgt catacttgag cgcgtgcttg acccagaccg cgcccgcata gcaggccgcg
    13321 ccagcgatga cgccgatgaa gaaggcgccc ttgggatcga cgaacccggc cgcaggagtg
    13381 atggcgacca gaccggcgac gatgccggac agcaggccga gcagggtcgg cttcttcttc
    13441 tcgatccatt cgatggccag ccagcccagt gaagcagcgg cagcggccag cagggtgttg
    13501 aaacaggccg tgcccgccag cgcatcggcg gcgcctgcag acccggcgtt gaagccgatc
    13561 cagccgacca gcagcaggcc ggtgcctatg gcggtgtagg cgaggttggc gggcgccatg
    13621 ttgtcacgac cgaagccgta acgcggtccg agcaccagac agcagaccag acc
//
LOCUS       DASVTM010000263         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_342785,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000263 DASVTM010000000
VERSION     DASVTM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342785"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1297
                     /locus_tag="VF633_07250"
     CDS             23..1297
                     /locus_tag="VF633_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8470890.1"
                     /translation="MSAARRLRNIVGGSAGNLVEWFDWYAYAAFTLYFAPVFFPDEDP
                     TAQLLSAAAVFAVGFLMRPVGAWIMGVYADRKGRKAGLTLSVSLMCAGSLIIGCSPGY
                     AQIGLAAPVLLLFARLLQGLSVGGEYGSSATYLSEMATPKHRGFWSSFQYVTLISGQL
                     LALLLLILLQNTLSETALASWGWRIPFFVGALLAVVVFWLRRRLDETSAFKAEDTGAA
                     PKSSALLLMRKHPKEALIVLGLTAGGTLAFYTYTTYLQKFLVNTSGFSKNTATEISAG
                     ALLIYMMLQPAVGALSDRIGRRPVMIAFGVLGVLCTVPIMSTLAVTTNPWAAFALALA
                     ALIIVSGYTAINAVVKAELFPAHIRALGVALPYAIANAVFGGTAEYVALWFKLDGNES
                     RYFWYVTVMIGVSLLVFVFMRDTKHKSLITHD"
     gene            complement(1635..2510)
                     /gene="phhA"
                     /locus_tag="VF633_07255"
     CDS             complement(1635..2510)
                     /gene="phhA"
                     /locus_tag="VF633_07255"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX8470891.1"
                     /translation="MSDFEHVFDAPPPGAAADWTISQNWRAYTEVEHDTWDTLYARQM
                     SILPGRASDAFLNGLKALDLNTGGIPDFAVINPKLQALTGWTVHCVPGLVPDDVFFDH
                     LANRRFVSGQFIRKPDQLDYLQEPDIFHDVFGHVPMLTDPDFADYMQAYGKGGQRAAS
                     LGMLPQLARLYWYTVEFGLMQSPKGLRIYGAGIVSSATESVFALDDASPNRIGFDLER
                     VMRTLYRIDDFQQVYFVIPSIEALRDVTLKDFGPVYEALRGQGDIAVDAILPDDRVLT
                     RGSQSYAARGGRFAA"
     gene            complement(2588..2902)
                     /gene="hspQ"
                     /locus_tag="VF633_07260"
     CDS             complement(2588..2902)
                     /gene="hspQ"
                     /locus_tag="VF633_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEX8470892.1"
                     /translation="MTQVQTAKFGLGQIVRHREQSFRGLIVDVDAVYTGPADQPTPDH
                     RNQPFYRVLAMGEDSGFLVYAAEGVLEVDPDINPLSREDYASWFTTDGAGHLAPRKQP
                     IQ"
     gene            3050..>3296
                     /locus_tag="VF633_07265"
     CDS             3050..>3296
                     /locus_tag="VF633_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470893.1"
                     /translation="MPEPDGAPPGRDDRSQRQRSDRASQPAQLRGDGAQGHAATGRDA
                     PLYGALDLGTNNCRLLIARPARDGFRVVDSFSRIVRLG"
BASE COUNT          560 a         1044 c         1079 g          613 t
ORIGIN      
        1 gacagagcca aagaccgaac cgatgtccgc cgcgcgacgt ctgcgcaaca tcgtcggcgg
       61 gtcggcgggc aatctggtcg agtggttcga ctggtacgcc tacgcggcct tcaccctgta
      121 tttcgcgccg gtcttctttc ccgacgaaga cccgaccgcc cagctactga gcgccgccgc
      181 cgtgttcgcc gtcggcttcc tgatgcgacc tgtcggggcc tggatcatgg gggtctatgc
      241 cgaccgcaag ggacgcaagg ccggactgac cctgtcggtc agcctgatgt gcgccgggtc
      301 cctgatcatc ggctgctcgc cggggtatgc ccagatcggt ctggccgcgc cggtattgct
      361 gctgttcgcg cggctactgc aggggctgag cgtcggcggg gagtacgggt cgtcggcaac
      421 ctatctgtct gagatggcga cgcccaagca tcgcggcttc tggtccagct ttcagtatgt
      481 gacgctgatc tcggggcagc tgctggcgtt gctgctactg atcctgctgc agaacacgct
      541 cagcgagacg gcgctggcgt cgtggggctg gcgcattccg ttcttcgtcg gcgcgctgtt
      601 ggccgtggtg gtgttctggt tgcggcggcg gctcgatgaa accagcgcgt tcaaggccga
      661 ggacaccggg gcggctccga aatccagcgc cctgctgctg atgcgcaaac accccaagga
      721 ggcgttgatc gtactgggcc tgacggctgg cgggacgctc gccttctata cctacacgac
      781 ctacctgcag aaattcctcg tcaacacctc cggcttctcc aagaacacgg cgaccgagat
      841 cagcgccggg gcgctgttga tctacatgat gctgcaaccc gccgtcggcg ccctgtcgga
      901 ccggatcggg cggcggccgg tgatgatcgc cttcggggtg ctgggcgtgt tgtgcaccgt
      961 gccgatcatg tcgacgctgg cggtgacgac caatccttgg gcggcgttcg cgctggcgct
     1021 ggcggcgctg atcatcgtct cgggctatac ggccataaac gccgtggtga aggccgagct
     1081 gttcccggcg cacatccgcg cgctgggcgt cgccctgccc tacgccatcg ccaatgcggt
     1141 gttcggcggc acggccgaat acgtcgccct gtggttcaag ctggacggca acgagagccg
     1201 ctatttctgg tacgtcacgg tgatgattgg cgtgtcgcta ctggtgttcg tgttcatgcg
     1261 agacacgaag cacaagagcc tgatcacgca cgactagcgt tctgccgtca tcggcattga
     1321 ggagtgaaga ctctcgaccc tgatcttctc gacctttccg accccgaaag agagacactg
     1381 aaccctccat cgccgatttt ctcgacaatc ggcgcccact gttcaggccg gtggtacaat
     1441 ggcccttcac agccgatctt tgacatcgcc aacctcgccg agccccgctg cacaggggtt
     1501 cgtcgctaac gcccgcatca agcgcggggc cggatcgccg gaaacagacg ccatccggtc
     1561 gcgcgacccg gcgccgccgt ttcggccaag ggtccatttc cggcattttt cggtgaacgc
     1621 gagactctcg accctcaggc cgcgaaacga ccgcctcgcg cggcataaga ctgcgagccc
     1681 cgcgtcagca cccggtcgtc cggcaggata gcatccactg caatgtcgcc ctgcccgcgc
     1741 agggcctcgt agaccgggcc gaaatcctta agcgtgacgt ctctcagtgc ctcgatcgag
     1801 gggatgacga aatagacctg ctggaagtcg tcgatccggt agagggtgcg cataacgcgc
     1861 tccagatcga agccgatccg gttgggcgac gcgtcgtcga gcgcgaacac gctctcggtc
     1921 gccgatgaga cgatcccggc gccgtagatg cgcaagccct tcggcgactg catcagaccg
     1981 aattcgaccg tgtaccagta cagccgtgcc agctgcggca gcatgccgag gctggcggcg
     2041 cgctggccgc ccttgccata ggcctgcatg tagtcggcga agtccgggtc cgtcagcatc
     2101 ggcacgtggc cgaagacatc gtggaagatg tccggctcct gcaggtagtc cagttggtcc
     2161 ggcttgcgga taaattgacc cgacacgaag cggcggttgg ccagatggtc gaagaagacg
     2221 tcgtccggca ccaggcccgg cacgcaatgc actgtccagc cggtgagggc ctgaagtttg
     2281 ggattgatca ccgcaaaatc aggaatgccg ccggtgttga ggtccagcgc cttgagcccg
     2341 ttcaggaagg cgtcggacgc ccgtcccggc aggatgctca tctgccgggc gtacagggtg
     2401 tcccaggtgt cgtgctcgac ctcggtatag gcgcgccaat tctggctgat cgtccagtcg
     2461 gcggccgcgc ccggaggcgg ggcgtcgaag acgtgctcga aatcggacat gtcaaactcc
     2521 actgggacgg ctgctgctga cctgcaatct aggcggcgat aacgggcgcg gctagtagac
     2581 cgcctcgcta ttggattggc tgctttcgcg gagcgagatg ccctgcgccg tccgtggtga
     2641 accatgaggc gtaatcctcg cggctcaacg gatttatatc cgggtcaacc tcaagcacgc
     2701 cctctgcggc atagaccaga aagcccgagt cctcgcccat cgccagaacg cgatagaagg
     2761 gctggttgcg atggtctggc gtcggttggt cggcgggacc ggtatagacg gcatcgacat
     2821 cgacgatcag gccacgaaaa ctctgctccc tgtgcctaac gatctggccg aggccgaact
     2881 tggctgtttg aacttgggtc atggcctgaa tgtaagcgat tggacatgag gctcgtccgt
     2941 ccgacctgtt catcctggcg tcatattcgc ttggcgagcg aaggcggacg aggctaggtt
     3001 gcccgggacg ggcgccgtca acggttgcgc ccatttgaaa gttcggccca tgccggaacc
     3061 agacggcgcg cccccggggc gcgacgaccg gtcccagcgc cagaggtccg accgcgcgtc
     3121 gcaaccggct cagctccggg gcgacggcgc acagggccat gcggcgacag gtcgcgacgc
     3181 accattgtat ggggcgctcg atctggggac caataactgt cgcctgctga tcgctcgtcc
     3241 ggcgagggat ggttttcgcg tggtcgacag cttcagccgc atcgtccggc tgggcg
//
LOCUS       DASVTM010000264         4826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_129670,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000264 DASVTM010000000
VERSION     DASVTM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129670"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="dusA"
                     /locus_tag="VF633_07270"
     CDS             <1..889
                     /gene="dusA"
                     /locus_tag="VF633_07270"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX8470894.1"
                     /translation="ERLLGFDKMEHPVALQLGGSDPVQLAEAARIGQAFGYNEINLNV
                     GCPSDRVQSGKFGACLMREPELVADCMAAIREAVSVPATVKCRIGVDDQDPATSLFAT
                     VDASAAVGITTFIVHARKAWLKGLSPKENRDVPPLDYALVRQLKRERPHLTICINGGI
                     ASLDAAATHLDASDGVQLDGVMLGRAAYHEPALLGQVDRRIFDEATPDVDPFAAIERY
                     KPYLAARLTEGVHLPAMTRHMLGLMHGRRGARAFRRILTVDSIASAAGIEVVERAVDA
                     VREAERAFEANQPASDRAA"
     gene            complement(927..1448)
                     /locus_tag="VF633_07275"
     CDS             complement(927..1448)
                     /locus_tag="VF633_07275"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX8470895.1"
                     /translation="MIRFDFQTVAVMAMATASSLSVAFWLSHADAQGAAHAETVTPMS
                     ASGTPARGRAAQVLKAADGHYWAEANIEGRAIHVLVDTGASVVALTREDASRLGLKLA
                     PEDFTRTVQTASGPARAAAVTLKSIAVAGAEVEDVQALVVENGLSQSLLGMSFLGRLS
                     GFEATPVALILRP"
     gene            complement(1566..1808)
                     /locus_tag="VF633_07280"
     CDS             complement(1566..1808)
                     /locus_tag="VF633_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX8470896.1"
                     /translation="MSVISGPPTADHTAGSAPRSIATPCVKVCIVDGSSSLCLGCYRT
                     LEEIGGWSGLTDVQRAQIMAELPARRDRIDPAKLGL"
     gene            1958..2113
                     /locus_tag="VF633_07285"
     CDS             1958..2113
                     /locus_tag="VF633_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470897.1"
                     /translation="MNDKDRNLQHAMTSFAFWGGMLVNSGRQSRANDRVVSHPSNFVP
                     IEIKRPR"
     gene            2219..3034
                     /locus_tag="VF633_07290"
     CDS             2219..3034
                     /locus_tag="VF633_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8470898.1"
                     /translation="MAAFIAQEKIAQPGSGSVLVLTFDRAERLNALPDLTDGDEVAAI
                     CEAVNADPDIRCVVLTGAGRAFSAGGDLNAMKARSDMFAGSGAAIREQYRRVVHRIVR
                     SLYGLEVPLIAAVNGPAFGLGCDIAGLADIRIASDRASFGVPFLKLGIIPGDGGSWLL
                     PRNIGYARAAEMLFTARAIDSETADKWGLVNRVVPHDALMNEALITAAQVAAQPPRAV
                     RMAKSLLRQGRDMNFDQMLELSAAMQALAHLTDDHIEGVTAVLEKRTGVFTGD"
     gene            3338..3985
                     /locus_tag="VF633_07295"
     CDS             3338..3985
                     /locus_tag="VF633_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX8470899.1"
                     /translation="MPTPIKIDFVSDVSCPWCVIGLAGLETAIERARDLVDVDIHFQP
                     FELNPQMAPEGENIVEHIARKYGSTPAQSASARTMIRDRAAETGFEMRMSDDSRIYNT
                     FDAHRLLHWAEEYGKQATLKAALFEAYFTKQMNPGDPEVLADTAALAGLDRAEAVEVL
                     SSGRYTAEVRAAEELWLQRGINSVPAVVINDKYLISGGQPAEVFEQALRQIAAEA"
     gene            complement(4004..>4826)
                     /locus_tag="VF633_07300"
     CDS             complement(4004..>4826)
                     /locus_tag="VF633_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX8470900.1"
                     /translation="IISVAARRGGIIKEVLVQEGDRVVAGQILARQEDDEARLAVQSS
                     RASVAQAESQLASLRVDINTAQREYDRLAKLVATNFVAGARMDQARDAIATSQARLGS
                     QAAAVQTARAQLAQAQYNQELTIIRAPAAGRIARRYANPGAGASTLQVSAMFDLEPDA
                     PRIARAEIVESDIPNVANGQAVEITPEGDPSKVYIGSVLRRAAVFGARKLASDDPSQR
                     SDERVVEVVVSAGDAPLLIGQRVLVKFMKPGQTAGAPRTVTSGVSADRAMRPQGS"
BASE COUNT          901 a         1522 c         1581 g          822 t
ORIGIN      
        1 cgagcggctg ctgggcttcg acaagatgga gcatcccgtg gcccttcagc tgggaggatc
       61 ggatcccgtc caactggccg aggccgcgag gatcggccaa gcgttcggtt acaacgagat
      121 caacctcaac gtcggctgtc cttcggaccg ggtgcagtca ggaaaattcg gcgcctgtct
      181 aatgcgcgaa ccggagttgg tcgccgactg catggctgcg atccgcgagg cggtgagcgt
      241 accggctaca gtcaaatgcc gtatcggcgt cgacgaccag gatcccgcga ccagcctgtt
      301 cgccacggtc gacgcctcgg ctgcggtggg catcaccacc ttcatcgttc atgctcgcaa
      361 ggcctggcta aaaggcctgt cgcccaaaga aaatcgcgac gtaccgccgc tcgactatgc
      421 gctcgtgagg cagttgaagc gcgagcgacc ccacctgacg atctgcatca acggcggcat
      481 cgcgtcactg gacgctgctg cgacgcacct cgacgcttct gatggcgtcc agctggacgg
      541 ggttatgctc gggcgcgccg cctatcacga gcccgcatta ctggggcagg tcgatcgcag
      601 gatcttcgac gaggcaacgc cggacgtcga tccgtttgcc gccatcgagc gatataaacc
      661 ctatctggcc gcgcgcctca ctgagggtgt ccatctgccc gcaatgacgc ggcacatgct
      721 gggcctgatg catggtcgcc ggggcgcgcg cgccttccgt cgcatcctga ccgtcgactc
      781 catcgcttcc gccgcgggga tcgaagtcgt ggagagggcc gtggacgcgg tgcgcgaggc
      841 tgaacgggcg tttgaagcca accagccggc gagcgaccgg gcagcctgag cggtccagga
      901 accagccagt ccagacttgt atccgctcag ggacgaagta tcagcgcgac aggcgtagcc
      961 tcaaagccgg acagtcggcc cagaaagctc attccgagca gcgattggga aagaccgttc
     1021 tcaaccacca aagcctgaac atcctcgacc tcggcgccag ccaccgcgat cgatttcagg
     1081 gtgacggctg cggctcgagc gggaccggat gccgtctgca cggttcgggt gaaatcttcg
     1141 ggcgccagct tcaggcctag acgggaggcg tcctcacgcg tcagggctac gacgctggct
     1201 ccggtatcga ccaggacgtg gatggcccgg ccctcgatgt tcgcctcggc ccagtagtgg
     1261 ccgtcggcgg ctttgagcac ctgagcggcg cggccacgag cgggcgttcc ggaagccgac
     1321 atcggggtaa cggtttccgc atgggctgcg ccctgcgcat cagcatggct cagccagaag
     1381 gcgacgctca gcgaagaggc cgtagccatc gccatgacgg cgactgtctg aaaatcgaag
     1441 cggatcatgg cctcacgcga ctgaaaccgc ttctgcggcc agatcaccct gtcacacagg
     1501 ccttgggatg cggtgaaccg acgtggctga cgaaaggtta gcgagcgtag ccttgacgac
     1561 cgccgtcaaa gacccagttt cgccggatcg atccggtcgc gacgagcggg gagttcagcc
     1621 atgatttgcg cacgctgtac gtccgtcaga ccagaccaac caccgatctc ctcgagcgta
     1681 cggtagcaac cgagacaaag gctggacgac ccgtccacga tgcagacctt gacgcagggg
     1741 gtagcaatcg atcggggcgc ggagccagcc gtgtggtccg cggtcggagg cccggaaatg
     1801 acagacataa gaaaactact ccggacccag accaaaacgc ttgcgtttta tcgctccgtg
     1861 tgcgaactag aattcgacgc gaaacgacta agagggtgca aacccctgca agcacgacgt
     1921 gtctcttttc ctcgttcaac gaaaggagac atcaacgatg aacgacaaag acaggaatct
     1981 ccagcacgcc atgacgtctt tcgccttctg gggcgggatg ttggtgaact ctggacggca
     2041 aagccgggcg aatgatcgcg tggtcagcca cccatccaac ttcgtgccga tcgagatcaa
     2101 gcgccctcgt taacccgggg gctctcgaca cggactgaag cgccaaaggc aagttccgac
     2161 ttgcaccgct acgccccggc cccgacaagg ccggggtttc gcaatttcgg agatcgccgt
     2221 ggccgccttc atcgcccagg agaaaatcgc tcaaccgggc agcggatcgg tgctggtcct
     2281 gaccttcgac cgggccgagc ggttgaacgc cctccccgat cttacggacg gtgatgaggt
     2341 cgcagcgatc tgcgaggcag tcaacgcaga tccggacatc cgctgcgtgg tcctgacggg
     2401 cgccggccgg gccttctccg ccgggggcga cctcaatgcc atgaaggcgc ggtcagacat
     2461 gttcgccgga tcgggcgcag ccatccggga gcaatatcgc cgcgtcgtcc accgtattgt
     2521 gcgctcgctc tatggtcttg aggtcccgct gatagcggca gtgaacggcc ctgcgttcgg
     2581 cctgggttgc gacatcgccg gcttggccga catccgcata gcctccgaca gggccagctt
     2641 cggggtaccg ttcctgaagc tgggcatcat tcccggcgac ggcggttcgt ggctattgcc
     2701 gagaaacatc ggctacgccc gcgccgccga gatgctgttc accgcccggg cgatcgactc
     2761 tgaaaccgcg gacaaatggg gtctcgtcaa tcgggtcgtg ccacatgatg cgttgatgaa
     2821 tgaggcgctg atcactgcag cccaggtcgc cgcccagccg ccccgggccg tgcggatggc
     2881 caagtcgttg ctacgtcagg gtcgcgacat gaatttcgat cagatgctgg agctcagcgc
     2941 cgcgatgcag gctctcgcgc atctgaccga cgaccacatc gagggtgtga ctgcggtgct
     3001 ggaaaagcgc accggcgttt tcaccggcga ctgacaaagc atgtcgcggt ccgcaatccc
     3061 ggcgcctcgc ctgagacggc gctggaaggt cctcagtcga atccggccga catcgacctg
     3121 ttcgggcagg tctcgggaag cggatcgtcg ggccgactgg cccctgccga tatccgacag
     3181 gcacgccggg ttcccttatg cccagatggc gccttgccag gactgacttg ctggagcgat
     3241 caacggtgca ggacattcag aggacaggct aagccgcgtt ggcgataccc ggatcggttc
     3301 cccatatgct gatggacgaa cgccagggaa ctcagctatg ccaaccccga tcaagatcga
     3361 cttcgtgtcc gacgtatcct gtccctggtg cgtaatcggg ctggcgggac tggagaccgc
     3421 gatcgaacgg gccagggatc tggtcgacgt ggatatccat ttccagccgt tcgagttgaa
     3481 cccacagatg gcgcccgagg gcgagaacat cgtcgagcac atcgcccgca aatacggatc
     3541 tacgcccgcg cagtctgctt cggcccgtac catgatccgt gaccgcgccg ccgagacagg
     3601 cttcgagatg cggatgagcg atgacagccg gatctacaac accttcgacg cccaccgact
     3661 gctgcactgg gcggaagaat acggcaaaca ggcgactctg aaggcggcgt tgttcgaggc
     3721 ctatttcacg aagcaaatga atccgggcga cccggaggtt ctggcggata cggcggccct
     3781 ggccgggctg gatcgagccg aagcggtcga ggtgctgtcg tctggtcgct atacggcgga
     3841 ggtacgggcg gctgaggaac tctggcttca acgcgggatc aattccgtcc cggcggtcgt
     3901 gatcaacgac aaatacctga tctcgggcgg ccagcctgcg gaggtgttcg agcaggcgct
     3961 gcggcagatt gcggccgagg cctgagcctc ggccgcctgc agtttagctt ccctgcgggc
     4021 gcatggcccg gtcggcgctg acgccgctgg tgaccgtgcg cggggccccc gcggtctggc
     4081 cgggcttcat gaacttcacg aggacccgtt ggccgatcag caggggcgca tcaccggcgg
     4141 agacgacgac ttcgacgacc cgctcgtccg aacgttgcga cgggtcatcg gaggccagtt
     4201 tgcgggcgcc gaagacggcc gcgcggcgca gtaccgagcc gatgtagacc tttgacggat
     4261 caccctcggg cgtgatttcg accgcttgcc cgttggcgac gtttggaatg tccgactcga
     4321 cgatttcggc ccgggcgata cggggcgcat caggctccag atcgaacatg gcggaaacct
     4381 gcagggtcga agcgcctgca cccggattgg cgtagcgccg ggcgatacga ccagccgcgg
     4441 gcgcccggat gattgtcagt tcctgattat actgagcctg agcgagctgg gcgcgggcgg
     4501 tctggacggc ggcggcctgc gagcccaggc gagcctgaga ggtggcgatg gcgtcacggg
     4561 cctgatccat gcgggcgccg gcgacgaaat tcgtggcgac cagtttcgcg agacggtcgt
     4621 actcacgctg ggccgtgttg atgtcgacgc gcagcgaggc cagttggctc tcggcctggg
     4681 cgacggacgc acggcttgac tggacggcga gacgggcctc gtcgtcttcc tgacgggcca
     4741 ggatctggcc ggctaccacg cggtctcctt cctggaccag aacctccttg atgatcccgc
     4801 cgcgacgcgc cgctaccgag atgatg
//
LOCUS       DASVTM010000265         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1035491,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000265 DASVTM010000000
VERSION     DASVTM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035491"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /gene="rpmB"
                     /locus_tag="VF633_07305"
     CDS             <1..277
                     /gene="rpmB"
                     /locus_tag="VF633_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX8470901.1"
                     /translation="GIGPMVGHSVSHSNVKTKRRFLPSLKKVKLASESLGQSFTLRIS
                     NAALRTLDYKGGLDVYIVKAGDEQLSLTAQRIKRQVKAKLAEQAAAA"
     gene            complement(414..836)
                     /locus_tag="VF633_07310"
     CDS             complement(414..836)
                     /locus_tag="VF633_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX8470902.1"
                     /translation="MSDPVETAPEYPPLNTLTTGIRCRCPRCGKGPLLQGYLTIRKNC
                     PVCGLDYSFADPADGPAFFVMSGVGVVGMMAFMAFEFSVHPPIWVHFAVTFPLIALMC
                     LATLRPFKGWIVSEQYVHKAAPPEWESIGKHGDGSPWR"
     gene            1045..3927
                     /locus_tag="VF633_07315"
     CDS             1045..3927
                     /locus_tag="VF633_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470903.1"
                     /translation="MAGSSSRKSIARTSVALGAVGAVVLAPILGGVAPAMAQEVSQAS
                     DPVTIRLGTSSRAEAGAPGGNFTRVEFAGVIGSRSRIRREGQAVVIRIGSTAAPDISL
                     LKNSPPPGVDKVETRAVTGATELVLTLAAGAEARSGVDDGAVWLNLYAPGAAPVVAAS
                     NAVPANGVVPVTTTADADKVVLNFQWAAPVGAAVFRRGDAVWIVFDVAARMGLPKGAK
                     LGPAGEARWAKGPDYTAIRIPAPQGLPVSATGHGATWSVTIGGSPTSPAQAVEVDRDD
                     SAQTRLVARMAGATKAIWLTDPMVGDRFAAVTALAPGKGFGDRRQTVDVTLLPTAQGL
                     AVETATDDLTITPQGDLVTVSRPRGLTMSAPSATLDTAPDAPHLPRKAEYPAMILAEW
                     ADAGSEGFPARYRQLQDGAEQETIQSTEDPRTPIESRLAFARFLIASGLGFEGIGVLN
                     AMIARTPSLQGEPEIRGLRGAARASIGRYDESEADFASGALAGDPSTRVWQGYVAVQH
                     GDWAGARQAFAAGASVIDQFPNDWKARFGAAHALAALELGDLPAARSLLAYSFSQNAP
                     AADQLTARLVQARLFELDGQSDRALAVYKAVARAPLDGIATPAKLGVVKISLAKGLMT
                     PDAAAAQLEQLKWRWRGDATELTVIRTLGGIYLSQGRYREALEVLRGAGKRMGDLPGS
                     TELQADLANTFRALFLQGAADGLQPVQALALFYDFRDLTPIGADGDEMVRRLARRLID
                     VDLLDQAAELLKHQVEERLDGVAKAQVATDLATVYLMDRQPEKALQALWGSRTTLLPT
                     AMNAERRALEARALMNLGRYDHAMETLDGDNTPDALDVRAEVFWKQQNWIAAAKIYDQ
                     RMGERFKDTPTPLSADEENRIIRAGVGYSLGHDLTSLTKLSRAYQPFVDRARSAAALR
                     IALAGFDGMEGVDAKDFASLTSGADTFAGWVQTMKADFRKQTGGNG"
     gene            complement(3973..>4118)
                     /locus_tag="VF633_07320"
     CDS             complement(3973..>4118)
                     /locus_tag="VF633_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX8470904.1"
                     /translation="SNLGVFDTADRAEREGRNPATGATIKIAASKAVRFRVSKPLKDA
                     VNA"
BASE COUNT          690 a         1361 c         1365 g          702 t
ORIGIN      
        1 cggtatcggc ccgatggtcg gccacagcgt gagccactcg aatgttaaga ccaagcgccg
       61 ctttctgccg tcgctgaaga aggtcaagct ggcttcggaa tccctgggcc agtccttcac
      121 cctgcggatc tcgaacgccg ccctgcgtac cctcgactat aagggcggcc tggacgtcta
      181 tatcgtcaag gctggcgatg agcagctgtc gctcacggcc caacgcatca agcgtcaggt
      241 caaggccaag ctggccgagc aagccgccgc cgcctgattt cggcgtctgc ctgaagattt
      301 gaaaaggccg gtggaaacat cggccttttt ttatcctctc cacgcagtgg ggggaggggg
      361 cagccgcaca ctcggtctct cgcgcgctcc ttcttatccg ataacgaacg cctttaccgc
      421 cagggcgacc cgtcgccgtg tttgccgatg ctttcccact ccggcggcgc agccttgtgg
      481 acatactgct cggacacgat ccagcccttg aacggccgca gggtcgcgag gcacatcagg
      541 gcgatcaacg ggaaggtcac cgcgaaatgc acccagatcg gcgggtggac gctgaactcg
      601 aaggccatga aggccatcat cccgaccacg cctacccccg acatgacgaa gaaagccggg
      661 ccgtctgcag ggtcggcgaa gctgtagtcg agcccacaga ccgggcagtt tttgcggatc
      721 gtcagataac cctgcagcag agggcccttg ccgcaacggg ggcagcggca gcggataccc
      781 gtcgtcaacg tattgagcgg ggggtattcg ggagcggtct cgaccggatc tgacatagcg
      841 tttcctttgg cctgatctcg gcctgcctgt agcgagacgc aaggcgccgc cgtgtccttg
      901 ggcgatttgt ccaacctgcg atcctcgagt ttagcggact atccggtcaa ccgttccgca
      961 gaacctgtgg gacaccctgc gctgacacgc cgcacacgcc tcattaaccg cgttgaaagg
     1021 ccacacgcgc gagtgtgcgc gtagatggcg ggctcttcct cgagaaaatc gatcgcgagg
     1081 acctctgtgg cgctgggcgc cgtgggggcg gtggtgctcg ccccgattct tggcggcgtc
     1141 gcgccggcca tggcgcagga ggtgtcccaa gcctccgatc cggtcaccat ccgccttggc
     1201 accagcagcc gcgcggaggc cggcgcccct ggcggaaact ttacgcgcgt cgaattcgcc
     1261 ggggtcatag gatcacgttc gcggatccgt cgtgagggcc aggcggtcgt tatccgcatc
     1321 ggctccaccg ctgcacctga catcagcctg ctgaagaaca gcccgcctcc cggcgtcgac
     1381 aaggtcgaga cccgagccgt caccggcgcc accgagctgg tcctgaccct cgccgccgga
     1441 gccgaggccc ggtctggtgt tgacgatggg gcggtctggc tgaacctcta cgcccccggc
     1501 gccgcgccag tcgttgcagc gtcgaacgcg gtcccggcca acggcgtcgt tcccgtcaca
     1561 acgacagccg atgccgacaa ggtcgtgctg aacttccagt gggcggcccc cgtcggcgcg
     1621 gcagtgttcc gccggggcga cgcagtctgg atcgtattcg acgtcgccgc ccgaatgggt
     1681 ttgcccaagg gcgccaaact tggccctgcc ggcgaagcgc gctgggccaa ggggcccgac
     1741 tacacggcca ttcgcattcc cgcgccccaa gggctgccgg tctcggcgac aggccatggc
     1801 gcgacctgga gcgtgaccat cggtggttcg ccgaccagcc ccgcgcaggc ggtggaagtc
     1861 gatcgcgacg acagcgccca gacccgtctc gtggcccgga tggccggggc gaccaaggcg
     1921 atatggctga cggatcccat ggtcggcgac cgcttcgccg ccgtcaccgc tctcgcgccc
     1981 ggcaagggct ttggcgaccg gcgccagacc gtcgatgtga ctctcttgcc gacggctcag
     2041 ggtctcgcgg tcgagacggc tactgacgat ctcaccatca cccctcaggg cgatctcgtc
     2101 accgtgtccc gcccgcgcgg gctgacgatg tcggcgccgt cggcgaccct cgatacagcg
     2161 cctgacgcgc cgcacctgcc ccgcaaggct gaatatccgg cgatgattct ggccgagtgg
     2221 gccgatgccg gttcggaggg ttttcccgcc cgttaccgtc agcttcagga tggggccgaa
     2281 caggagacga tccagtccac cgaagatcct cgtacgccga tcgagtcgcg actggccttc
     2341 gcccgcttcc tgatcgcgtc gggcctcggc ttcgagggca tcggcgtcct gaacgccatg
     2401 atcgccagga cgccgagcct gcagggcgag cccgagatcc ggggattgcg gggcgccgcc
     2461 cgtgcgtcca ttggccgata cgacgaatcg gaagccgact tcgcctcggg cgctctggcc
     2521 ggcgatccct cgacccgcgt ctggcagggt tatgtcgcgg tccagcacgg ggactgggcc
     2581 ggggcgcgtc aggcgtttgc agcgggcgct tcggtcatcg atcagttccc caacgactgg
     2641 aaggcccgtt tcggcgcggc ccatgcgctg gcggctctcg aactcggtga tcttccggcg
     2701 gcgaggtcgt tgctagccta cagcttcagc cagaacgccc ctgcggcgga tcaattgacg
     2761 gcgcgactgg ttcaggcgcg cctgttcgaa ctcgacggcc agagcgaccg cgcgttggct
     2821 gtctacaagg ccgtagcccg ggccccgctg gacgggatcg ccacgccggc caagctgggc
     2881 gtggtcaaga tatcactggc caaagggctg atgacgcccg acgcggccgc cgcccagctc
     2941 gagcagctaa aatggcgctg gcggggcgat gcgaccgagc tgaccgtcat ccggacgctg
     3001 ggtggcatct atctttctca agggcgctat cgtgaagcgc tcgaggtgct gcgcggcgcc
     3061 ggaaagcgaa tgggtgacct gcctggctca accgagcttc aggccgatct ggccaacacc
     3121 ttccgggcgc tcttcctgca aggtgctgca gacggtttgc agcctgtgca ggcgctggcc
     3181 ttgttttatg attttcgcga tctgacgcct attggggccg acggtgacga gatggtgcgt
     3241 cgactggcgc gccgcttgat cgacgtggat ctgctggacc aggctgccga gctcctgaag
     3301 catcaggtgg aggaacgcct cgatggcgtg gccaaggcgc aggtcgcgac agatctggcc
     3361 accgtctatc tgatggatcg tcagccggag aaggcgctgc aggccctgtg gggctcgcgc
     3421 acgaccttgc tgccgaccgc catgaacgcc gagcgccggg cgctggaggc gcgcgctctg
     3481 atgaacctcg gccgctacga ccacgccatg gaaacgctcg acggcgacaa cacgccggac
     3541 gcgctcgacg tgcgcgcgga ggtgttctgg aaacagcaaa actggatcgc cgccgcgaaa
     3601 atctatgacc agcgtatggg cgaacggttc aaggatacgc cgacacccct gagcgcagac
     3661 gaggagaacc ggatcatccg cgccggtgtc gggtattcgc ttggccacga tctgacttcg
     3721 ctgacgaaac tgtcgcgcgc ctatcagcca ttcgttgacc gggctcgctc ggctgcggcc
     3781 ctgcgcatcg cattggccgg cttcgacggt atggagggcg tggacgccaa ggacttcgcc
     3841 agcctgacct cgggcgccga taccttcgcc ggatgggtcc agacgatgaa ggcggacttc
     3901 agaaagcaaa cgggcggaaa cggctgaccg tttccgcccg cgttcctcgt cgcaatgcga
     3961 cgtggagaag tcttacgcgt tcacagcgtc cttgaggggc ttggacacgc ggaagcggac
     4021 agccttggaa gcggcgatct tgatggtcgc gcccgtggca gggttacggc cttcacgctc
     4081 ggcgcggtcg gccgtgtcga aaacgcccag gttcgaga
//
LOCUS       DASVTM010000266         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_186135,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000266 DASVTM010000000
VERSION     DASVTM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186135"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2402)
                     /gene="rpoB"
                     /locus_tag="VF633_07325"
     CDS             complement(<1..2402)
                     /gene="rpoB"
                     /locus_tag="VF633_07325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX8470905.1"
                     /translation="MTDVLNGLAINGQIASATSFTGKKRIRKSFGRIPEAVAMPNLIE
                     VQRASYEQFLQREVRPGVRKEQGIEAVFRSVFPIKDFNERAVLEYVSYEFEDPKYDGE
                     ECIQRDMTYAAPLKVKLRLIVFEADEETGARSVKDIKEQDVYMGDIPLMTDKGTFIVN
                     GTERVIVSQMHRSPGVFFDHDKGKTHSSGKLLFAARVIPYRGSWLDFEFDAKDVVFVR
                     IDRRRKLPATTFLYALGMDGEEILRTFYETVPYEKRGEGWVTPYKPERWRGVKPEFDL
                     IDADTGEIVASAGAKISARAAKKLQDGGLKSLSLAADALITRYLAADAVNYETGEIYA
                     EAGDELDAESIALLESQGFTTIDVLDIDHVTVGAYMRNTLRVDKNTGRDDALFDVYRV
                     MRPGEPPTTEAAEAMFNSLFFDSERYDLSAVGRVKMNMRLETPEVSDETRVLQKDDVL
                     KVLQILVGLKDGRGEIDDIDNLGNRRVRSVGELLENQYRVGLLRMERAIKERMSSVDI
                     DTVMPHDLINAKPAAASVREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRER
                     AGFEVRDVHPTHYGRICPIETPEGPNIGLINSLATHARVNKYGFIESPYRRVKDGKAL
                     DEVVYISAMEESKYTIAQANIQLKDGMIVEELVPGRINGDSQLLIRADVDMMDVSPKQ
                     VVSVAAALIPFLENDDANRALMGANMQRQAVPLVQSDAPLVGTGMEAVVARDSGAVVV
                     ARRDGVVEQIDGTRIVVRATNELDAGRSGVDIYRLSKFQRSNQSTCINQRPIVRVGDE
                     VKV"
     gene            2822..3340
                     /locus_tag="VF633_07330"
     CDS             2822..3340
                     /locus_tag="VF633_07330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8470906.1"
                     /translation="MTSIARLALIATVAVGLTSGLAACASGPPETLLAAAPADAASWV
                     PAQRGYLAWNLTRRGWNVTPSGLQYKRVGRAFPKAPMPATTDNVRVHYRGTLITGTEF
                     DSSYSRNEPAEFPLNRVIKGWTEGVALMHVGETYDFVIPADLAYAGRRMGDDIPAEST
                     LLFRVQLLAINP"
BASE COUNT          615 a         1145 c         1096 g          676 t
ORIGIN      
        1 accttcactt catcgcccac acgcacgatc gggcgctggt tgatgcaggt cgactggttc
       61 gaacgctgga acttggacag gcgatagatg tcgacgcccg aacggccggc gtccagttcg
      121 ttggtcgcgc ggaccacgat ccgggtgccg tcgatctgtt cgacgacgcc gtcacgacga
      181 gcgaccacga cagcgccgga gtcacgcgcc acgaccgctt ccatgccggt gccgaccaga
      241 ggcgcgtcgg actgcaccag aggcacggcc tgacgttgca tgttcgcgcc catcaatgcg
      301 cggttggcgt catcgttttc gaggaacggg atcagggccg cagcgaccga aacgacctgt
      361 ttcggtgaca cgtccatcat gtcgacgtcg gcgcggatca gcagttgcga gtcaccgttg
      421 atccggcccg ggaccagctc ttcgacgatc atgccgtcct tgagctgaat gttcgcctgg
      481 gcgatcgtgt atttcgactc ttccatggcc gagatgtaaa ccacctcgtc cagggccttg
      541 ccgtccttca cgcgacggta cgggctctcg atgaagccgt atttgttcac gcgggcgtgg
      601 gtggccagcg agttgatcag gccgatgttc gggccttccg gcgtttcgat cgggcagatg
      661 cggccgtagt gagtcgggtg aacgtcacgg acttcgaagc cggcgcgttc acgcgtcaga
      721 ccgccagggc caagcgccga aaggcgacgc ttgtgagtga tttcggacag cgggttcgtc
      781 tggtccatga actgcgacag ctgcgacgag ccgaagaatt cacgaaccga ggcggcggct
      841 ggcttagcgt tgatcaggtc gtgcggcatc accgtgtcga tatcgaccga cgacatgcgc
      901 tccttgatcg cgcgctccat acgcagcagg ccgacgcggt actggttttc cagcagttcg
      961 ccgaccgaac ggacccggcg gttgccgagg ttgtcgatgt cgtcgatttc gccacggccg
     1021 tccttcaggc cgacgaggat ctgcaggacc ttcaacacgt cgtccttttg caggacgcgc
     1081 gtctcgtcgg acacttccgg cgtttccaga cgcatgttca tcttgacccg accaacggcc
     1141 gacaggtcat agcgttcgct gtcgaagaac agcgaattga acatggcttc ggcggcttcc
     1201 gtggtcggcg gctcaccggg acgcatcacg cgatagacgt cgaacagagc gtcgtcgcgg
     1261 ccggtgttct tgtccacgcg cagggtgttg cgcatgtagg cgccgaccgt gacgtggtcg
     1321 atgtccagca cgtcgatggt ggtgaagccc tgcgactcca gcagtgcgat cgactcggcg
     1381 tccaattcat caccggcttc ggcgtagatt tcgccggtct cgtagttgac ggcgtcggct
     1441 gccagatagc gggtgatcag ggcgtcagcc gccagcgaca gcgacttcag gccaccgtcc
     1501 tgcagcttct tggcggcgcg ggcgctgatc ttggcgccgg ccgaggcaac gatctcaccg
     1561 gtgtcggcgt cgatcaggtc gaactcgggc ttaacaccgc gccagcgctc gggcttgtac
     1621 ggggtgaccc agccttcgcc acgcttctcg taagggacgg tttcgtagaa cgtgcgcagg
     1681 atttcctcac cgtccatgcc gagggcatag aggaaggtgg tcgcgggcag tttgcggcga
     1741 cggtcgatac gaacgaacac aacgtccttg gcgtcgaact cgaagtcgag ccacgaacca
     1801 cggtaaggaa tgacgcgggc ggcgaacagc agcttgcccg acgagtgggt cttgcccttg
     1861 tcgtggtcga agaacacgcc cggcgaacgg tgcatctgcg agacgatcac gcgctcggtg
     1921 ccgttgacga tgaacgtacc cttgtccgtc atcaggggga tgtcccccat gtagacgtcc
     1981 tgttccttga tgtccttgac cgagcgggcg ccggtttctt catccgcctc gaacacgatc
     2041 aggcgcagct tgaccttcag cggcgcggca taggtcatgt cgcgctggat gcattcctcg
     2101 ccgtcgtact tcgggtcctc gaactcgtag gagacgtatt ccagaacggc gcgttcgttg
     2161 aagtccttga tcgggaagac cgaccggaag accgcctcga tgccctgctc cttgcggacg
     2221 cccggacgga cttcgcgctg caggaactgc tcgtaggagg cgcgttgaac ctcgatcagg
     2281 ttcggcatcg ccaccgcttc ggggatacgc ccaaaggatt tgcggatgcg cttcttgccg
     2341 gtgaagctcg tggccgaggc gatctgaccg ttgatggcga gaccgtttag gacgtctgtc
     2401 attcagtgtt tcccattccg gacagcgtga cctgccggag aaatgcagtg gcacggcgcg
     2461 ccatccggcg accgtgcgtt tcggtttcgg cttccaccct cggaccccaa aaggtccagg
     2521 aagcctgaaa tctgtgagct ccctgggata ggagccacgc cttcgctcgg tcggagggcg
     2581 acggaccggg cctcggcgct ttcgcgcgga atcatctaag tgattgcgga gagcggacat
     2641 atactcgctt ttccgacgaa ataaaggccg gtccacaagg aaatatgctg tcctgcggat
     2701 tcacgctttc ctcgcgccaa aatccctgca agcggccctg ccctacccgc agtctcggca
     2761 accgtcctgc gatcacatag cgccactttg cggcgacttt ggaccctgct agcgtcgccc
     2821 catgacatcc atcgcccgcc ttgccctgat cgccaccgtc gccgtcggcc tgacctccgg
     2881 tctcgccgcc tgcgcctcgg gcccgcccga gaccttgctc gccgcagccc cggcggacgc
     2941 cgcctcctgg gtcccggccc agcgcggata tctggcctgg aacctgaccc gtcgcggctg
     3001 gaacgtcacc ccgtctggcc tgcagtacaa acgagtcggt cgcgccttcc ccaaagcgcc
     3061 aatgcccgcc accaccgaca acgttcgggt ccactatcgc ggcacgctga tcaccgggac
     3121 agagtttgac agctcctact cccgcaacga gcccgccgaa ttccccctca accgcgtcat
     3181 caagggctgg accgagggcg tcgccctgat gcacgtcggc gagacctacg acttcgtcat
     3241 ccccgccgac ctcgcctacg ccggccgccg catgggcgac gacatcccgg cggagtcgac
     3301 gctgctgttc cgcgtgcagc tgctggcgat caatccatag ggcgagctac cggctttcgg
     3361 agcgagagcg atggccgctg tcgacccatt gcatacctcg gtggactgtt gtcaggaagc
     3421 actcggccgc gatgtccgcc aaccccgtgg cggaactccg gaacggcagt tgcccatccg
     3481 cactagccag acattggagc gacagccgaa tagttctcca tttggtccac gg
//
LOCUS       DASVTM010000267         4177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_506351,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000267 DASVTM010000000
VERSION     DASVTM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506351"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VF633_07335"
     CDS             <1..1033
                     /locus_tag="VF633_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8470907.1"
                     /translation="VAFCGGGDVSVDRWDSSEHLDDDPRRCMPSGLSSAPRHETMSVM
                     DGAAAKALGDLARERWFRATWERTIPEEVDGDPWPDNVPVSITKVPVGIARSEPKWGG
                     RGEVRENEALHLKAIQKAKKLIYLENQYFTSPAVAACLAQRLAEDDGPEVVLVSTGNS
                     PSWFDKMTMDTARSEVMFRLESADKHNRFHAFAPYTKQGDRIIVHAKVSIIDDRLLRI
                     GSTNLNNRSMGLDTECDIAAEPTDQAGCDAIRAFRHRSIGHFIGVTPRDFAAAEAVLA
                     STGRAIQTFDTGRMELLGATPPSQIERFIAEWQLGDPSSSDNAWRPWKRRNPSQRVAG
                     GNFSQLRER"
     gene            complement(1037..1771)
                     /locus_tag="VF633_07340"
     CDS             complement(1037..1771)
                     /locus_tag="VF633_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8470908.1"
                     /translation="MPRLLTYNVHRCVGTDKRLDVARIVAVIAEHEPDIVCLQELDVG
                     RARTGGVDQAKAIAAGLEMTFHFNAAMMIEAEEYGDALLTRRPERLMRKGALPTMRGI
                     PGLEPRGAVWASIDFDGVAVNVINTHLGLVPREQRLQAAALTGKDWLGHADCVGPTIL
                     TGDFNSTSITRPYQTLTRGLADCQRQLGLKQTVKTFPSGFPAIRIDHAFVSPHIRVTG
                     VRAPFSPLARVASDHLPLVIDFEIDP"
     gene            complement(1850..2542)
                     /locus_tag="VF633_07345"
     CDS             complement(1850..2542)
                     /locus_tag="VF633_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX8470909.1"
                     /translation="MVTTFTPDDHARVGAAIAAAERTTSGEIFCVFARRVSSYRDVSL
                     GWAAAGALILPMLSIPLGFEAAWFPGLGDNWQAAHLAASHVTLGKTLAAYAVAQAAIF
                     VTVLLITCIPVVLRWVTPRRVRRARVRRAALQQFLAHGLHTTQYRTGVLIFAALADRQ
                     VEIIADQGIYSKVDAEIWAQAVDRLTVALRAGRAAEGFETAIALTGEVLATHFPPRAN
                     NPDELPNRLVEI"
     gene            complement(2536..3318)
                     /locus_tag="VF633_07350"
     CDS             complement(2536..3318)
                     /locus_tag="VF633_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX8470910.1"
                     /translation="MRSFWLLLAFWLAAVIPSAALADPTFPALSGRVVDEAHLLQPAA
                     RDSIVARLEKLEADTGDQLVVVTVPDLQGYEIEDYGYRLGRAWGIGQKENNSGALLIV
                     APTERKVRIEVGYGLEPVLTDAMSAQIIQSEILPAFRTGGFQRGISSGVDAIIAQLRL
                     DPAEAQARAEAAKPAEADVPTGPIILVGLVFLFILIGMIRASHGGGRRRRGGGVGPVI
                     LWSALNALSSSGRSVGGSFGSGGGGFSGGGGSFGGGGASGGW"
     gene            complement(3339..4010)
                     /locus_tag="VF633_07355"
     CDS             complement(3339..4010)
                     /locus_tag="VF633_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8470911.1"
                     /translation="MVRFPARTVAVALMVLPAVSGCGYNTIPTKQERAQAAWADVQSQ
                     YQRRADLIPNLVATVQGAAIAERTTLDAVISARARATSVQVTPETLSDPAAMQAFQAA
                     QGQLTGALSRLLATVEAYPNIQSNQNFVQLQSQLEGTENRITIARRDYNEAVRDYNTS
                     LRTFPTVIWAKTFQSGSKPMQPFTATAEAQTAPTVTFNVGPTVPAATPGGTAPAVTPG
                     SAPAQ"
BASE COUNT          752 a         1293 c         1450 g          682 t
ORIGIN      
        1 ggtcgccttc tgcggcggag gagacgtctc ggtggatcgc tgggattcat ccgaacatct
       61 ggacgatgat ccccgccgct gcatgccctc gggcttgagc agtgcaccgc gccacgagac
      121 catgtcagtt atggacggcg cagcggcgaa ggcgctgggc gacctggctc gcgaacgctg
      181 gttccgcgcg acctgggagc gaaccatccc cgaggaagtc gacggcgatc cctggcctga
      241 caatgttcct gtctcgatca ccaaggttcc ggtgggtatc gcccgcagcg aaccgaaatg
      301 gggcgggcga ggcgaggtgc gcgagaacga agccctgcat ttgaaggcga tccagaaagc
      361 gaaaaaactt atttatctcg agaaccagta ttttacttcg ccggccgtcg ccgcctgcct
      421 ggcccaacgt ctggccgagg acgacggacc ggaggtggtg ctcgtttcga ccggcaacag
      481 tccgagctgg ttcgacaaga tgaccatgga cacggcccgc tccgaggtga tgttccggct
      541 tgagagcgcc gacaaacaca atcgctttca cgcgttcgca ccttacacga aacagggcga
      601 ccggatcatc gtgcacgcca aggtctccat catcgatgac cggctgctgc ggatcggatc
      661 gacgaacctg aacaaccggt cgatgggcct cgatacggaa tgcgatatcg cagccgagcc
      721 gaccgatcag gcgggctgcg acgccattcg cgcctttcgc catcgaagca tcgggcattt
      781 catcggggtg acgccgaggg atttcgcagc ggcggaagcc gtgctggcgt cgacgggacg
      841 ggcgatccag accttcgata ccggccggat ggaactgctg ggcgcgacgc cgccctcgca
      901 aatcgagcgg ttcatcgccg aatggcagtt gggcgatccg tcgtcgtcgg ataacgcctg
      961 gcgtccgtgg aagcgccgca atccgtcaca gcgtgtcgcc ggcggcaact tcagccagct
     1021 tcgcgagcgc tgacgttcaa gggtcgattt cgaaatcgat gaccagcggc aggtgatccg
     1081 aggcgacacg cgccagtggt gagaacggcg cccgaacacc cgtgacgcgg atgtgcgggc
     1141 tgacgaaggc gtggtcgatg cggatcgccg ggaaacctga cgggaacgtc ttcacggtct
     1201 gcttgagacc caattggcgc tgacagtcgg ccaacccgcg cgttagcgtc tggtagggac
     1261 gggttatcga ggtggagttg aagtcaccgg tcaggatagt ggggccgacg caatctgcat
     1321 gacctaacca gtccttgccg gtcagcgccg ccgcctgcag tcgttgctcg cgcgggacga
     1381 ggccgaggtg ggtgttgatg acgttcaccg cgacgccgtc gaaatcgatc gaggcccaga
     1441 ccgcgccgcg gggctccagc cccggaatac cgcgcatggt cggcagggcg cccttgcgca
     1501 tcagccgctc ggggcgtcgg gtcaacaggg cgtcgccgta ctcctcggcc tcgatcatca
     1561 tcgcggcatt gaagtggaaa gtcatctcga ggccggcggc gatggccttg gcctgatcca
     1621 cgccgccggt gcgggcgcga cctacgtcca gttcctgaag acagacgatg tcaggctcgt
     1681 gctcggcgat aacagccacg atccgggcca cgtcgaggcg tttgtcagtc ccgacgcagc
     1741 ggtgcacgtt gtaagtcaga agtcggggca tggagtccga aacggccgtt gcgcggcaga
     1801 gctaggccat ttccttcgcg attgcgaccc tcgtctggcc tgcgccagtc tagatctcga
     1861 cgagccggtt cggcagttcg tcgggattgt tcgccctcgg aggaaagtgt gtggcgagga
     1921 cctcgccggt cagggcgatg gcggtttcga aaccttccgc ggcgcgaccc gcgcgtagcg
     1981 cgacggtcag gcggtcgacg gcctgggccc agatctcggc gtcgaccttc gagtagattc
     2041 cctggtctgc gatgatctcg acctgacgat cggcgagggc ggcgaagatc aggactcctg
     2101 tacggtactg ggtcgtgtga agtccgtggg cgaggaattg ctgcaaggcc gcgcgacgaa
     2161 cccgcgcacg gcggacgcgg cgcggcgtga cccagcgcag caccaccggg atgcaggtga
     2221 tcagcagcac ggtgacgaaa atcgcggcct gggcgacggc ataggcagcg agcgtcttgc
     2281 cgagcgtgac atggctggct gcgagatggg cggcctgcca gttgtcgccg aggcccggga
     2341 accaggcagc ctcgaaaccg agtggaattg acagcatcgg caggatcagg gcccccgccg
     2401 cggcccagcc gagactgacg tcgcgatagg aggacacccg ccgcgcgaag acacagaaaa
     2461 tctcgccgga tgtcgtgcgc tcggcggccg ctatcgcggc gccgacccgt gcatgatcgt
     2521 ccggagtgaa ggtcgtcacc atccgcctga agctccgccg ccgccgaaac tgccgcctcc
     2581 gcctgagaag ccgccaccac cgctaccgaa gctaccaccg acggagcggc cgctgctgct
     2641 cagggcgttg agagcgctcc acaggatcac gggaccgacg cctccgccgc gccgccggcg
     2701 tccgccgccg tgcgaggcgc ggatcatgcc gatcaggatg aagaggaaga ccagaccgac
     2761 gaggatgatg gggcctgtgg gcacgtccgc ctccgccggc ttggcggctt cagcgcgagc
     2821 ctgagcctcg gcgggatcaa gtcgaagctg ggcgatgatg gcgtcaacac ctgaggagat
     2881 gccgcgctgg aagccgcccg tgcggaacgc cggaaggatt tcgctctgga tgatctgggc
     2941 cgacatggcg tcggtgagaa ccggctcaag gccatagccg acctcgattc gcaccttgcg
     3001 ctcggtgggg gcgacgatca gcagggcccc gctgttgttt tccttctggc cgatgcccca
     3061 ggcgcgaccg aggcgatagc cataatcctc gatctcgtag ccctgaaggt caggaaccgt
     3121 gaccacgacc agctgatcgc cggtgtccgc ctccagcttt tcaagccggg caacgatcga
     3181 atcgcgcgcg gcgggctgaa ggagatgagc ctcgtccacc acgcgaccgg acagagccgg
     3241 gaaggtcggg tctgcgagcg ccgccgaggg gatgacagcc gccagccaga aggccaacaa
     3301 caaccagaat gatcgcatcg gacctgggtt cagggctgtc actgagcggg cgcggagccc
     3361 ggggtgacgg cgggcgcggt cccgccgggt gtggccgccg ggacagtcgg gccgacgttg
     3421 aaggtcactg tcggggcggt ctgggcctcg gcggtcgccg taaacggctg catcggtttg
     3481 gagccggact ggaacgtttt ggcccagatg acagtcggga aggtccgcag cgaggtgtta
     3541 taatcgcgaa ctgcctcatt gtagtcgcgc cgggcgatgg tgatgcggtt ttcggtgcct
     3601 tccagctggg actggagctg gacgaaattc tggttcgact ggatgttcgg ataggcctcg
     3661 accgtggcca gcaaacgcga aagggcgccg gtcagctggc cctgtgccgc ctggaaggcc
     3721 tgcatcgccg ccgggtcgct gagggtctcg ggcgtcacct gcacagacgt cgccctggct
     3781 cgcgcactga tgactgcgtc caacgtggtg cgttctgcga tggctgcgcc ctgaacggtg
     3841 gccaccaggt tggggatcag gtcggcgcgt cgctgatact ggctctggac atccgcccag
     3901 gcggcctgcg cccgctcctg cttggtgggt atggtgttgt agccgcagcc ggatacggcc
     3961 ggcaggacca tcagggcgac ggcgactgtt ctagcgggaa aacggaccat gcagacatcc
     4021 tcgaccgggc cgccaacgcg acgaccgagt cggactatca gcggttaatg atgccgcttc
     4081 aagcgcctag cgataacggc gtgatgctat ttgcacgaca ggcggcgacg taggtgttcg
     4141 ccagcaggca ggcgatggtc atcgggccga cgccgcc
//
LOCUS       DASVTM010000268         2934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_186229,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000268 DASVTM010000000
VERSION     DASVTM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186229"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VF633_07360"
     CDS             <1..359
                     /locus_tag="VF633_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470912.1"
                     /translation="QKAQEAPAPATAPAAVAQTQAEIEAASQAAYDAEANTQPTDPVD
                     AFIWRTDLCMHLSGEIGGDRSERDVQVQAQMEGLKCADVVADGRALKVANASNPAAVK
                     NIEASLKAWTDYFGAP"
     gene            complement(412..1563)
                     /locus_tag="VF633_07365"
     CDS             complement(412..1563)
                     /locus_tag="VF633_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX8470913.1"
                     /translation="MAANLLRTRDGHHARVGFVELFFDLVFVFAVTQISHSLLHHLTW
                     LGALQAAMMAAAVWWAWIYTSWVTNWLDPERIPVRIALFVLMGLGLLMSTSLPEAFGE
                     RGLLFAIAFVATSVFRSGFMLWASRKDAVLPRNFQRILSWQLLSGAFWIAGGLMESEM
                     RIALWLAALAIDYAGPAAGYRVPGLGVANTADWTVEGGHMAERVGLFVIICLGESLLI
                     TGATFAATPWTTEVILAAAVAFVGALAMWWIYFSAAHDAASEVISHATDTGALARAAY
                     TYCPVLIVAGIIVTAVGDELSLVHPSGHVATATAAVLIGGPLLFMIGGLLAKVAVFGR
                     WSVPRTVGCIAIAALWLAVPLTTPLGLAALTTGVLIAVAAWETVRLRSQ"
     gene            1647..>2934
                     /locus_tag="VF633_07370"
     CDS             1647..>2934
                     /locus_tag="VF633_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8470914.1"
                     /translation="MDPSPPWWEQAAAMFVIVMLTGALVGPVFAPLQEETPILRLIWL
                     PVYAVITALLCYRADKVIRAWPAMLILLAMILHAYASKYWSIDPGVTVRRTVAMAFSS
                     VFAIYLGAVFRGPQLPRLLMLSGLLMGIGSLIMVFAFPRIGVHQFDNAGLWRGLWYEK
                     NQMGVVMVAGAIAAAACLALPGERTKMAWASFGIGVVLVLATQSKTSLLCLIIGVGLI
                     GTLWAMQKGGPALTVIGLWFGVVLVGIVGYVFITEPAVVLKALGKDPSLTGRTDIWVA
                     LMREVNLRPWTGFGYQAFWGRESIPAMWIRAETQWPVPSAHNGWIDLLIQLGWPGAVA
                     VGVTVLIAFLSTLFRIPGAGAREGYWGVGYFAVFLMLSLSESVLLTNANLPWTLLLAI
                     LARGLLVGQEPLMPRGRTKLAPQAGGAYLTRSRIASQ"
BASE COUNT          511 a          907 c         1029 g          487 t
ORIGIN      
        1 gccagaaggc gcaggaggcc cctgctccag caacggctcc tgcagcggtt gcgcagacac
       61 aggccgagat agaggcggcg agtcaggcgg cttacgacgc cgaggcgaac acacagccga
      121 ctgatccagt ggacgccttc atctggcgca ccgatctgtg catgcatctg agcggggaga
      181 taggcgggga tcgatccgag cgcgacgtcc aggtgcaggc gcagatggaa gggttgaagt
      241 gcgctgacgt ggtcgcagac gggcgggcgc taaaggttgc caatgcctcg aaccccgctg
      301 ccgtgaagaa tatcgaggcg tcgctgaagg cctggaccga ctatttcggc gccccctgat
      361 ccttcccggc attgccctgc cttgaggcga cgccggccca gggccgctcg gctactgaga
      421 ccgcaatcga accgtctccc aggccgcgac ggcgatcaga acgcctgtcg tcagggccgc
      481 gaggcctagc ggtgtggtga gggggaccgc cagccacagc gcggcgatag cgatgcagcc
      541 gaccgtccgc ggaaccgacc agcggccgaa cacggcgacc ttggccaaca gacctccgat
      601 catgaacagc agcgggccgc cgatcaggac agccgccgtc gcggtggcga cgtggccgga
      661 cggatggacc agcgagagct cgtcgccgac ggccgtgacg atgatcccgg cgacgatcag
      721 gaccgggcaa taggtatagg ccgcgcgagc cagggcgccg gtgtcggtcg cgtgcgagat
      781 gacctctgac gcggcgtcgt gggcggcgct gaaatatatc caccacatgg ccagagcgcc
      841 gacgaaggcg acggcggcgg cgaggatgac ctcggtcgtc catggcgtcg cggcgaaggt
      901 cgcgccggtg atgagcaagg attcaccgag gcagatgatg acgaacaggc cgacgcgctc
      961 agccatgtgg ccgccctcga cggtccagtc ggcggtgttg gcgacgccga gaccggggac
     1021 gcgatagccg gcggcaggcc cggcatagtc gatggccaga gcggcgagcc agagagcgat
     1081 ccgcatctcg ctttccatca gaccacccgc gatccagaag gcgccagaca gcagttgcca
     1141 gctgaggatg cgctgaaaat ttcgcggcag gacagcgtcc ttgcgcgacg cccagagcat
     1201 gaagccgctg cggaacacgg aggtggcgac gaaggcgatc gcgaacaaca gtccgcgctc
     1261 gccgaaggcc tcgggcaagg aggtggacat cagcaggcca agacccatca gcacgaacaa
     1321 ggcgatgcgg accgggatcc gctcggggtc gagccagttg gtgacccaac tggtgtatat
     1381 ccatgcccac cagaccgcgg ctgccatcat cgccgcctgg agtgcgccga gccaggtcag
     1441 gtggtgcagc aggctgtgtg atatctgggt gacggcgaag acgaagacga ggtcgaagaa
     1501 cagctcgacg aaaccgacgc gggcgtgatg gccgtcgcgc gtcctgagca gatttgcagc
     1561 cattcgcccc accaaatgcc aacggttcgt taagcgtaaa cgacgaccct gcgatcgtga
     1621 ccttctcccg tccagaacat gattccgtgg acccgtcgcc cccatggtgg gagcaggcgg
     1681 cggcgatgtt cgtcatcgtc atgctcacgg gggcgctggt cggaccggtg ttcgcgccgt
     1741 tgcaagagga gacaccgatc ctgcggctga tctggttacc ggtctatgcg gtgatcacgg
     1801 ccctgctctg ctatcgcgcc gataaggtga tccgcgcctg gcccgcgatg ctgatcctgc
     1861 tggcgatgat cctgcacgca tacgcctcga aatactggtc gatcgatccc ggcgtgacag
     1921 tgcgtcggac cgtggccatg gccttctcca gcgtgttcgc catctatctg ggcgccgtat
     1981 tcagggggcc gcagctgccc cggctgttga tgctgtcggg tctgctgatg gggatcggca
     2041 gcctgatcat ggtgttcgcc ttcccgagga tcggcgtgca ccagttcgac aacgccgggc
     2101 tgtggcgcgg tctctggtat gaaaaaaacc agatgggcgt ggtcatggtc gcgggcgcga
     2161 tcgccgccgc cgcgtgtctg gcgctgccgg gcgagcggac gaagatggcc tgggccagtt
     2221 tcgggatcgg cgtcgttctg gtgctggcga cgcagtccaa gacctcgctg ttgtgtctga
     2281 tcatcggcgt cggcctcatc ggaaccctgt gggcgatgca gaagggcggg cctgcactga
     2341 cggtgatcgg cctgtggttc ggtgtcgttc tggtcgggat cgtcggctat gtcttcatca
     2401 ccgaaccggc cgtggtgctc aaggcgctgg gcaaggatcc gtcattgacc gggcggaccg
     2461 acatctgggt cgccctgatg cgcgaggtga acctgagacc gtggaccgga ttcggctatc
     2521 aggccttctg gggccgcgag tccattcccg ccatgtggat ccgcgccgag acccagtggc
     2581 ctgtgccgtc ggcgcacaat ggctggatcg acctgctgat ccagctgggg tggccgggcg
     2641 cggtcgcggt cggcgtcacc gtcctgatcg ccttcctgag cacgctgttc cgcattccgg
     2701 gcgcgggggc gcgtgagggg tattggggcg tcggctattt cgccgtgttc ttgatgctga
     2761 gcctgtcgga gagcgtcttg ctgaccaacg ccaacctgcc gtggacgctg ctgctagcga
     2821 tccttgcccg tgggctgctg gtcggtcagg agccgcttat gccaagaggc cggacgaagc
     2881 ttgccccgca ggctggcggg gcctacctca cgcggtcccg aatcgcctca cagt
//
LOCUS       DASVTM010000269        12944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_663714,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000269 DASVTM010000000
VERSION     DASVTM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663714"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(822..895)
                     /locus_tag="VF633_07375"
     tRNA            complement(822..895)
                     /locus_tag="VF633_07375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(861..863),aa:Gly,seq:ccc)
     gene            complement(960..2321)
                     /locus_tag="VF633_07380"
     CDS             complement(960..2321)
                     /locus_tag="VF633_07380"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX8470915.1"
                     /translation="MAATEPLSKDQLIAVMSDGAKPKDQWRVGAEHEKFGFDRSTLRR
                     PAYDGPSGIHAMLTGLQRFGWSPVEEGGHLIALERKNAEGFMASVSLEPGGQFELSGA
                     PLLTMHDICNETGQHLMEVKQVADQINLGFLGAGFDPLWTREDVPVMPKGRYDIMRSY
                     MPKKGKLGLDMMLRTCTIQSNLDFADEADMVAKFRTSLALQPIATALFACSPFIEGKP
                     SGFLSARANVWTDTDPDRTGMIGFVFEDGFGFERYADYALDTPMYFAKRDGKYIDASG
                     QSFRDFLKGELPALPGELPTIKDWNDHLTTLFPEVRLKSYLEMRGADGGPWSRICALP
                     ALWTGIFYDAPSLAAAWDLCKDWDIEDHERLRRDVTRLGLTAEVAGRSVRDIAVDMVA
                     IASAGLKARNCLSGGMVDERGYLSELEDIADSGLTPADRMLALYHGEWQGDVSRIYRD
                     FAY"
     gene            complement(2418..3140)
                     /locus_tag="VF633_07385"
     CDS             complement(2418..3140)
                     /locus_tag="VF633_07385"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX8470916.1"
                     /translation="MIRLYVTTDLAPDAAVAPTPDQSRYLTGVMRLKQGEDLLVFNGR
                     DGEWRASIAEYLKRGVVLKCLEQVRPQATGPDLELIVAVVKKARVETIVEKAAELGVA
                     RVRLTMTRRTNVDHVRLDRLDAIAIEAAEQTGRLDVPLVDDPVKLDEILNDWDPDRRL
                     MFCDETGGAPAIPALTAAGPGPWAILIGPEGGFSPEEGERLRSLPFTTAVSLGPRILR
                     ADTAAIAAMTLWQAAVGDWARD"
     gene            complement(3155..4333)
                     /locus_tag="VF633_07390"
     CDS             complement(3155..4333)
                     /locus_tag="VF633_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8470917.1"
                     /translation="MNVITTPSPDFMLEEEITLFSDSVGKWIDEHAPPEAVQSWIANS
                     SVPRQLWNDAGEAGLLGLSMPEEDGGMGGDYRHEVVLMRQLGWKGADHFGISLHNAIV
                     APYIWHYGTEEQKQRWLPRLATGELVGAIAMTEPGAGSDLQGVKTTAIKSGNGYVVNG
                     SKTFITNGQLANFIIVVAKTDPSLGSKGTSLIVVETDGDAGGNVEGFERGRNLHKIGM
                     EGNDTSELFFNDVQVPGDNIIGGGEGQGFIQLMQQLPQERLNIAVQGVAAAERGLEQT
                     LEYVKQRKAFGKSILEFQNTQFKLAEVKTKLTVARVFVDHCIGLHLKGQLDAVTASMA
                     KYWVTDIQGEVIDEMLQLHGGYGYMNEYPIAQLYKDARVQRIYGGTNEIMKVLIARSL
                     "
     gene            complement(4508..6289)
                     /locus_tag="VF633_07395"
     CDS             complement(4508..6289)
                     /locus_tag="VF633_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8470918.1"
                     /translation="MAYKAPVRDLTFILNEVLEIDRYSNQTGFEDISSDLVGQILEEG
                     AKFAEEVIAPINNPGDKEGCHWNDGVVTGPKGWKEAYKAMVEAGWPALSADPAYGGQG
                     MPSVVGMAFGQFTAGASAAFSMYPGLTAGAHAGIHANASEELKAKYLPKMTTGEWGGT
                     MNLTEPQCGTDLGMVRTKAIPQGDGTYKITGQKIWISAGEHDFTDNIIHTVLARVEGA
                     PAGIKGLSLFLVPKILVNEDGSLGERNGVRCGGLEHKMGIHGNATCVMDYDEATGWLI
                     GQEGRGMNNMFVVMNEARLGTGLQGLAIGAAAYQGAVTFAKDRLQGRALTGPQNPTGP
                     ADSIMVHPDVRRMLLEARAFVEGGQAFILWTALQADLQKSSDEATAQKAHDYMGLLTP
                     VLKAYLTDKGFHVASLSMQVHGGSGYTEEFMASQYLRDARITMIYEGTNGIQALDLVG
                     RKLPSQGGRAIMSWFADVDAFVAENAGNESIKTYVDGLAATKAQLQDGTMWLMQNGLA
                     NPDNAAAASTDYLNLFGITALAYLWAQMAKAAQAKIDAGDTDPFYVTKLQTGRYFVER
                     ILPDAAAHLAKMKTGADVLMAMPAEAF"
     gene            complement(6293..6805)
                     /locus_tag="VF633_07400"
     CDS             complement(6293..6805)
                     /locus_tag="VF633_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8470919.1"
                     /translation="MSTADDHRTYSIRQLCREFGATARALRFYEDKGLLTPARKGQTR
                     VYDARDRARLKLILRGRRIGFTLQEIQDMLDLYDRNDGNTHQMAIALRRHRAQIEALK
                     QQREDLDGAIEMAEAACAAMEERLTNTRPDLLPGAEEYESIVSARIDHDHHHHDFAAH
                     PARHPFKARA"
     gene            6989..7165
                     /locus_tag="VF633_07405"
     CDS             6989..7165
                     /locus_tag="VF633_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HEX8470920.1"
                     /translation="MARKHVNDAGTAKRELPQKTCVSCGKPFAWRRKWARDWDQVKFC
                     SDRCRSAEVGRSAD"
     gene            complement(7162..7599)
                     /locus_tag="VF633_07410"
     CDS             complement(7162..7599)
                     /locus_tag="VF633_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8470921.1"
                     /translation="MAATFTSASLVRSTTPRDRTIATWRNLALWTLQGWIAMFFIAAG
                     YAKITESMDNLIVLMNWPALVSESFVRGVGIAEIVLAIGVLAPLASWKFGRPILIVAT
                     AGLIALETIMLRVHALGGDISLAIVNVFLLAITIPVLIGRRAS"
     gene            complement(7906..9369)
                     /locus_tag="VF633_07415"
     CDS             complement(7906..9369)
                     /locus_tag="VF633_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX8470922.1"
                     /translation="MPVTSAYRPEPRFFNLGDEFADAVQAADFPQTRIRYRNDRAAAT
                     VGLDDLSDAEWITAFGRFQPLPGQPGPLAMRYHGHQFRNYNPDLGDGRGFLAAQLRET
                     PSAQAASGRAASDSALPGRLLDLGTKGSGTTPWSRSGDGRLTLKGGVREVLAATMLES
                     QGVPTSRALSLIETGEALERGDEPSPTRAAVLVRLQHSHVRFGTFQRAAWFQRADMIE
                     TLVEHVRSLYHPAVAEGDAPGLLRAIVEASAQLTARWIAAGFVHGVLNTDNLNVTGES
                     FDYGPWRFLPYYEPGFTAAYFDQTGLYAFARQPEAVFWNLTQLAGCLKLVADAGLLTE
                     ALNGFGPAYIRELRAAFLMRLGVKSLGEAADQRLTDATLALLREGGTALRWEPLFFDW
                     FAGFSSSARALSGPRAATYQGEAFDVFRFALFEHEPERPERLEHPMFAAANPQEMLIE
                     EVETIWAAIGEADDWTPFEAKLARLEDARQGWDFAAD"
     gene            9570..10781
                     /gene="nhaA"
                     /locus_tag="VF633_07420"
     CDS             9570..10781
                     /gene="nhaA"
                     /locus_tag="VF633_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX8470923.1"
                     /translation="MARIFTLDFLKTETASGAALGIAAVAALVVANSPLAGEYFSWLK
                     SYHVLQIGPLRLEESISNWIKEGLMAVFFLVVGLEIKYEILRGELSDPRKLATPVLAA
                     LGGMVAPALVYLAISSATGGPHGGWPVPLATDIAFALAVFAMVGKNLPPSLRVFLLTL
                     AIVDDLGAIALIAVLFSQGVHLMPLLGVLALLVTGAIWSGVRRIPAPFWVMGFAAVWY
                     LTVQAGISTSLSAVAFALIVPIKPRTEDQQSPLKEAMHDLHPYVAWLVLPLFAFAKAG
                     VSFAGLSLDQAMAPLVLGIAAGLFLGKQIGVFGAAWLASKLNIGARPTDATWLQVYGV
                     SLLCGVGFTMSLFIGALAFPGAIDSPEQVEVKIGVLAGSLLSAFAGALALTIANLPSR
                     KVLARTGPDTA"
     gene            11025..>12944
                     /locus_tag="VF633_07425"
     CDS             11025..>12944
                     /locus_tag="VF633_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470924.1"
                     /translation="MRLTQILRSTTSAAVVGAAVFGFAGIASAQTADQDGPTATVDDI
                     IVTAQKREQSLQDVPIVVTSLNQEALNNAGVKDIKDLQILTPGMTVTSTTSEASTTVR
                     IRGVGTVGDNPGLESSVGVVIDGVYRSRNGVGFGDLGELERIEVLKGPQGTLFGKNTS
                     AGVVNIITEAPSFTPGYNAELTWGNFNALGASGSVTGPINDQVAFRLYAARRTRDGFN
                     PVDIGDGPRSQRDDQNQDFWTARGQVLILPNDNTSIRIIADYSKRDEYCCTGTQVRTG
                     QTYPFIDGLSTGTGQRPPNATFSRLPFSRTGFANNGTEQEIEDRGISAEGNFDLTSFG
                     GATLTTVTSWRQWDSVNGQDIDYTGADILHRDPNGDYGYRVGNITQEVRLAGQTDKLD
                     WLVGAFATREHIDRTDSYIFGPDYTPFLSFLLSANLNRAVPAFPVSPNDIGCFTRTAQ
                     TALGLQQCLGTGGAVSGGGIGFITGAGVRDSYSQDSDSVALFTNNSFHVTDKLDITVG
                     LRYTYDSKSLDALQTNQNGNSNTCAAAGANAGAIVGVLGAAGQTILGTICLPWANAAF
                     NNRATHQEESDGEFSGTVKAAYRFNPSLMTYVSYARGYKSFGYNLDRVQSGVTPLPST
                     FFPSELVDSYEAGFKA"
BASE COUNT         2239 a         4032 c         4274 g         2399 t
ORIGIN      
        1 agtcctgatc tattcgtatt gcctgagacg ccgggcgtag gactgtcagg gttggcctga
       61 ttcaagaggt ggggcccacc gccgggctgg cggccgcgaa tcttcacgac cgatattcgc
      121 tttggaatgg tgaagcttcg cgatccgcct caccaagggg gcgggtgcgc tgaaggccat
      181 gacggcgtcc atgcaaaatc gacccgatca accggggcca aagatgtccc cgtcatgccc
      241 gcgttccaaa gctgggacgc tgcctggccg catcaaggca tagcgacagt ttaaacgtct
      301 gtgctcttgc gaaccgttct tatagcgcgc catgtcaagg ggtcgtccct ttgtgcgcac
      361 aaccgtcggc gaccggcggt ccgccccgaa ccttaactgg ctccgggacg ggccgttaga
      421 ccaacgaggc ggcctccgcc tgacttccgg ctaccctagg gcaatccagg atcctgagac
      481 ggatactgcg ggaactgtgc tgatgcgctg gcttcgaccg cggtccgctg ccgctgcgcc
      541 tccgccaagc ccagcctagg cccagaaata gcgtggcccg ggttgggcgc acgaccggca
      601 agcggccatg gtccgtctcc agattgtgga tgggtctgtg gacgagggaa caaccgcgct
      661 cactgctcgc tgcggtctct cagatcgagg tgaccgatgc gcgtatggct atgtgaccag
      721 tggacggcct gggttcggga agggctgcca aggcggctct gaccctcttt gagtggcggc
      781 gtggcattga taagccgggc actgcccaag aggctaagtg ctggagcggg cggcgggaat
      841 cgaacccgca cgaaaagctt gggaagcttt caggctacca ctacatcacg cccgcggaag
      901 cgaggggcaa actagcgatt accgtagcgg gctcaagacc tgattgatca gggacggatt
      961 cagtaagcga agtcgcggta gatgcggctg acgtcaccct gccattcgcc gtggtagagc
     1021 gccagcatgc ggtcggcagg tgttagcccc gagtccgcga tgtcttccag ttcggacagg
     1081 tagccgcgct cgtcgaccat gccgccggac aagcagttac gggccttgag gccggcggag
     1141 gcgatggcga ccatgtcgac ggcgatgtcg cgcacggagc ggccggcgac ctcggccgtg
     1201 aggcccagac gggtgacgtc gcggcgcagg cgttcgtggt cctcgatgtc ccagtccttg
     1261 cacaggtccc aggcggcggc gagcgatggg gcgtcgtaga agattccggt ccacaaggcg
     1321 ggcagggcgc agatccggct ccacggtccg ccatcggcgc cgcgcatctc cagataggat
     1381 ttcagccgca cctcggggaa gagggtggtc aggtggtcgt tccagtcctt gatcgtgggc
     1441 agctcgccgg gcaaggcggg cagttcaccc ttgaggaagt cacggaacga ctggcccgag
     1501 gcgtcgatgt atttgccgtc gcgcttggcg aaatacatcg gggtgtcgag ggcgtagtcg
     1561 gcgtatcgct cgaagccgaa accgtcctcg aagacgaagc cgatcatgcc ggtgcggtcg
     1621 ggatcggtgt cggtccagac gttggcgcgc gccgacagga agccggacgg cttgccctcg
     1681 atgaagggcg aacaggcgaa cagggcggtg gcgatcggct gcaacgccag ggacgtgcgg
     1741 aacttggcga ccatgtcggc ctcgtccgcg aagtcgaggt tggactggat ggtgcaggtg
     1801 cgcagcatca tgtcgaggcc gagcttgccc ttcttgggca tgtaggaccg catgatgtca
     1861 tagcggccct tgggcatgac cggcacgtcc tcgcgggtcc acagcgggtc gaaaccggcg
     1921 ccgaggaagc cgagattgat ctgatcggcg acctgtttga cctccatcag atgctggccg
     1981 gtctcgttgc agatgtcgtg catggtcagg agcggcgcgc ccgacagttc gaactggccg
     2041 ccgggctcga gcgagacgga ggccatgaag ccttcggcgt ttttgcgttc gagggcgatc
     2101 aggtgaccgc cttcctcgac agggctccag ccgaagcgct gcaggccggt gagcatggcg
     2161 tggatgccgc tggggccatc ataggcgggg cggcgcaaag tcgaacgatc gaagccgaat
     2221 ttctcgtgct cggcgccaac gcgccactgg tccttgggct ttgcgccatc ggacatgacc
     2281 gcgatcagct ggtcttttga aagcggctcg gtagcggcca tgcgtcggtc gtccatctcc
     2341 gacgtgcgtc ggcctgtcgc ggctaaatgg gcaggaatac ggctgcgctc aagaggcgac
     2401 gcgcatcatt cgcgaactca atcgcgggcc cagtctccga cggccgcctg ccacagcgtc
     2461 atggcggcga tggccgcggt gtcggcacgc aggatgcggg ggccaagcga cacggccgtg
     2521 gtgaaaggca gggaccgcaa acgctcgcct tcttccggtg agaatccgcc ctcgggaccg
     2581 atcaggatcg cccacggacc gggtccagcc gccgtcagcg ccgggatggc cggagcgcct
     2641 ccggtctcat cgcagaacat cagccgccgg tcgggatccc agtcgttgag gatctcatcg
     2701 agtttcacgg ggtcatcgac cagtggcacg tccagccgtc cggtctgttc ggcggcctca
     2761 atggcgatgg cgtcgagccg gtcgaggcga acatgatcga cgttggtccg gcgggtcatg
     2821 gtcaggcgca cgcgagcgac gccaagttcg gcagccttct cgacgatggt ctcgacgcga
     2881 gccttcttga ccacggcgac gatcagttcg aggtcgggcc cggtggcttg cggacgcacc
     2941 tgctccaggc atttgaggac gacgccgcgc ttgagatatt cggcgatgct cgctcgccac
     3001 tcaccatcgc gtccgttgaa cactagcagg tcttcgccct gcttcaggcg catcacgccg
     3061 gtcagatagc gggattggtc cggcgtcgga gcgacggcgg cgtcaggagc gaggtcggtg
     3121 gtgacgtaaa ggcggatcat gacgcctcaa tacctcagag cgaccgcgcg atcagcacct
     3181 tcatgatctc gttggtgccg ccgtagatgc gctggacgcg ggcgtccttg taaagctggg
     3241 cgatcgggta ttcgttcatg tagccgtagc cgccgtggag ctggagcatc tcgtcgatga
     3301 cctcgccctg gatgtcggtg acccagtatt tggccattga ggcggtgacg gcgtcgagct
     3361 ggcccttgag gtgcaggccg atgcagtgat cgacgaacac gcgggccaca gtcagcttgg
     3421 tcttgacctc ggcgagcttg aactgggtgt tctggaactc gagaatcgac ttgccgaagg
     3481 ccttccgctg tttgacgtat tcgagggtct gttcgaggcc gcgctcggcc gccgcgacgc
     3541 cttgaacagc gatgttcagg cgttcctgag gcagttgctg catcaactgg atgaagccct
     3601 gaccttcgcc gccgccgatg atgttgtcgc ccggcacctg cacgtcgttg aagaacagtt
     3661 cggaggtgtc gttgccctcc atgccgatct tgtggaggtt gcgaccgcgc tcgaagccct
     3721 cgacgttacc gcctgcgtct ccgtcggtct cgaccacgat cagggacgtg cccttcgagc
     3781 ccaggcttgg gtcggtcttg gcgacgacga tgatgaagtt ggcgagctgg ccgttggtga
     3841 tgaaggtctt ggagccgttg acgacatacc cattgcccga cttgatcgcg gtcgtcttga
     3901 cgccctgcag gtcggagccg gcaccgggct cggtcatggc gattgcgccg accaactcgc
     3961 ctgtggccag acgcggcagc cagcgctgct tctgctcctc ggtgccgtag tgccagatgt
     4021 agggggcgac gatggcgttg tgcagcgaga tgccgaaatg gtccgcaccc ttccagccga
     4081 gctgacgcat cagcacgacc tcgtggcgat aatcgccgcc catgccgccg tcttcctcgg
     4141 gcatcgacag gccgagcagg ccggcctcac cggcgtcgtt ccacagctgg cgcgggacgc
     4201 tggagttggc gatccatgac tggaccgctt cgggtggcgc gtgctcgtcg atccatttgc
     4261 cgacgctgtc ggagaagagg gtgatctcct cttccagcat gaagtctggc gacggggtgg
     4321 tgatgacgtt catggcgttt ccagtcattt cttgagccct tcccccttga tgggggaagg
     4381 gttgggatgg gggtggaggc gccggacgtt aagtgatgca gtaccgcatc cctgacggtc
     4441 actcgccgta caggttgtca cccccaaccc cggcccttcc ccatcaaggg ggaagggtgc
     4501 agtcagatca gaaggcctcc gctggcatgg ccatcagcac gtccgcgccg gtcttcatct
     4561 tggcgaggtg cgccgcggcg tctggcagga tgcgctcgac gaagtagcgg ccggtctgca
     4621 gcttggtgac atagaacgga tcggtgtcgc cggcgtcgat cttcgcttga gcagccttcg
     4681 ccatctgggc ccagagatag gctagggcgg tgatgccgaa caggttcaga tagtcggtcg
     4741 aggcggcggc ggcgttgtct ggattagcca gaccgttctg catcagccac atggtgccgt
     4801 cctgcagttg ggccttggtc gcggcaaggc catcgacata ggtcttgatg gattcgttgc
     4861 ccgcgttctc ggccacgaag gcgtcgacgt cggcgaacca gctcatgatc gcgcgaccgc
     4921 cttgcgacgg cagcttgcgg cccacgaggt ccagcgcctg aatgccgttg gtgccttcat
     4981 agatcatggt gatccgcgca tcgcgcagat actggctggc catgaactcc tcggtgtaac
     5041 ccgaaccgcc gtgaacctgc atcgacagcg aggcgacgtg gaagcccttg tcggtcagat
     5101 aggccttcag caccggggtc agcagaccca tgtagtcgtg ggccttctgg gcggtagctt
     5161 cgtcggagga cttctgcagg tcggcttgca acgcggtcca cagaatgaac gcctggccgc
     5221 cttcgacgaa ggcgcgagcc tccagcagca tgcgacgcac gtcggggtgg accatgatgg
     5281 agtcggccgg gccggtcggg ttctgcggac cggtcagggc gcggccctgc aggcggtcct
     5341 tggcgaaggt cacggcgccc tgataggcgg cggcgccgat ggccagaccc tgtaggcctg
     5401 tgccgaggcg ggcctcgttc atcacgacga acatgttgtt catgccccga ccttcctgac
     5461 cgatgagcca gccggtcgcc tcgtcatagt ccatgacgca ggtggcgttg ccgtgaatgc
     5521 ccatcttgtg ctcgagccca ccacagcgga cgccgttgcg ctcacccagc gagccgtcct
     5581 cattgacgag gatcttgggc acgaggaaca gcgacaggcc cttgatgccg gccggagcgc
     5641 cttcgacgcg ggcgagaacc gtgtggatga tgttgtcggt gaagtcgtgc tcaccggccg
     5701 agatccagat cttctggccc gtgatcttgt aggtgccgtc gccctgcggg atggccttgg
     5761 tgcgcaccat gccgaggtcg gtgccgcact gaggctccgt caggttcatc gtgccgcccc
     5821 actcgccagt ggtcatcttg ggaaggtatt tggccttcag ctcttccgag gcgttggcgt
     5881 ggatgcctgc atgggcgccc gccgtcaggc cggggtacat cgagaaggcg gccgaggcgc
     5941 cggcggtgaa ctggccgaac gccatgccga cgaccgacgg catgccttga ccgccgtacg
     6001 cggggtcagc cgacagggcg ggccaaccgg cctcgaccat cgccttgtaa gcttccttcc
     6061 agcccttggg accagtgacg acgccgtcgt tccagtggca gccttccttg tcgcccgggt
     6121 tgttgatcgg ggcgatgact tcctcggcga atttggcgcc ttcttccagg atctggccga
     6181 cgaggtccga cgagatgtcc tcgaaaccgg tctggttcga atagcgatcg atctccagca
     6241 cttcattgag gatgaaggtc aggtcgcgga cgggcgcctt gtaggccatg gattacgctc
     6301 ttgctttgaa aggatgacgc gcggggtggg ccgcgaagtc gtggtggtgg tgatcatggt
     6361 cgatgcgggc cgaaacgatc gactcgtatt cctcggcgcc cggcagaagg tcgggccggg
     6421 tgttggtcag ccgctcttcc atcgcggcgc aggcggcttc ggccatctcg atggcgccgt
     6481 ctaggtcttc gcgctgctgc ttcagagcct cgatctgggc ccggtggcga cgcagggcga
     6541 tggccatctg atgggtattg ccgtcgttgc ggtcgtacag atccagcatg tcctggatct
     6601 cctgaagggt gaagccgatg cgacgtcccc tcaggatcag cttgagccgg gcgcgatcac
     6661 gggcgtcata gacgcgggtc tggcccttgc gcgcaggggt cagcaggccc ttgtcttcat
     6721 agaaacgcag cgctcgcgcc gtggccccga actcgcggca caactggcgg atggagtagg
     6781 tgcggtgatc gtcggctgtc gacatgggag cgtcataact tgacgcgcac gtaaagggaa
     6841 ggtttttcct ccccatccct tcagatgggg agggggacca cccggcgctt gggtggtgga
     6901 ggggttcttg aagccacgcg gccgaaaaac ttccttggag ccttatcttt cgggcggggt
     6961 tagccgtctc cgcaggcatg gaaacccaat ggctcgaaag cacgtcaacg atgcggggac
     7021 cgccaaacgc gaattgccgc agaagacgtg cgtctcctgc ggcaaaccgt ttgcctggcg
     7081 ccgaaagtgg gcgcgcgatt gggatcaggt gaagttctgt tcggaccgct gccggtccgc
     7141 cgaagtgggc cggtccgctg attagctagc gcggcggccg atcaggacgg ggatcgtgat
     7201 cgccagcagg aagacgttga cgatggccag gctgatgtcg ccgcccagag cgtggacgcg
     7261 cagcatgatg gtctcaagcg cgatcagacc agcggtcgcc acgatcagga tcggacggcc
     7321 gaacttccac gaagcgaggg gagcaaggac gccgatcgcc agcacgatct cggcgatgcc
     7381 gacgccgcgc acgaagcttt cgctgaccag agccggccag ttcatcaata cgatgaggtt
     7441 gtccatcgac tcggtgatct tggcgtagcc ggcggcgatg aagaacatcg cgatccagcc
     7501 ctgcagggtc cagagcgcga ggttgcgcca cgtggcgatc gtgcgatcgc gcggggtcgt
     7561 cgaacgaacg agggaggcag acgtgaaggt agcggccatg acgggacctg aaaaaagatg
     7621 ggggcttcgg ggaggacgct tactggtatc tgcaacagcc acgatatcgg atcgacaacc
     7681 gctgttcgca agggatgtat ggcagaggtt tatcggaatg tcatcagccg tagacatcag
     7741 ttgatgactt aggcgatcac ggtgtgtttc atgcacgggg gccggtgttt tctcacggcc
     7801 ttgcacatct cgcctctgcc gataacgttc atggcgcagc tacgcggcgg aagcgtggcc
     7861 atatgcatga atgcgtcatc ttttcccgat ccgggaaaga acccatcagt cggcggcgaa
     7921 atcccagccc tgacgggcgt cctccaggcg agccagcttg gcctcgaacg gggtccagtc
     7981 gtctgcctcg ccgatagcag cccagatcgt ctcgacttcc tcgatcagca tttcctgcgg
     8041 gttcgctgcg gcgaacatcg gatgctccag ccgctcgggc cgctcgggct catgttcgaa
     8101 cagggcgaag cggaaaacgt cgaaggcctc gccctgatag gtcgcagcgc gcggtcccga
     8161 cagcgcccgg gcagacgagc tgaaaccggc gaaccagtcg aagaaaagcg gctcccagcg
     8221 tagggcggtg ccgccctcgc gcagcaaggc cagcgtggca tcggtcagtc tctggtccgc
     8281 cgcctcgcca agcgatttga cgcccagccg catcaggaag gccgcccgca gctcgcggat
     8341 ataggcgggg ccaaacccgt tcagcgcctc ggtcaacaga cccgcatcgg cgaccagttt
     8401 caggcaaccg gcgagctggg tcaggttcca gaagaccgcc tcaggctgac gcgcaaaggc
     8461 atagaggccc gtctgatcga aataggcggc ggtgaagccg ggttcgtagt agggcaggaa
     8521 gcgccagggg ccgtagtcga agctctcgcc ggtgacgttc agattgtcgg tgttgaggac
     8581 gccgtggacg aacccggcgg cgatccagcg ggctgtcaat tgcgcggagg cttcgactat
     8641 tgcgcgcagc aggcccggcg catcgccctc cgccacggcc ggatggtaga gcgagcgcac
     8701 atgctcgacg agagtctcga tcatgtcggc gcgctggaac caggctgcgc gctggaaggt
     8761 cccgaagcgc acgtggctgt gctgcagccg aaccagaacc gctgcacgcg taggggaggg
     8821 ttcgtcgcca cgttccagcg cctcgccggt ctcgatcagg ctcagcgccc ggctggtcgg
     8881 cacgccctgg ctctccagca tggtcgcggc gagcacctcg cgaacgccgc ccttcagcgt
     8941 cagccggcca tcacccgagc gcgaccaggg cgtcgtgccg gagccttttg ttcccaggtc
     9001 gagaaggcga cctggaagag cgctatcgct cgcggcgcgg ccgctcgctg cttgagcgct
     9061 aggtgtttcg cgcaactgtg cggcgaggaa gccgcgtccg tcgccgaggt cgggattgta
     9121 gttgcggaac tggtggccgt gatagcgcat ggccaagggc ccgggctggc cggggagggg
     9181 ctgaaaacga ccgaaggctg tgatccattc ggcgtcgctg aggtcgtcca gcccaaccgt
     9241 cgccgccgcc ctgtcgttgc gatagcgaat ccgggtctgg gggaaatctg cggcctgaac
     9301 cgcatccgcg aattcgtcgc caaggttgaa gaagcggggc tcagggcgat aggcggaggt
     9361 cacgggcatg aggcgcagat gcggcgggca ggcccttggt tcaagggctc tgacgagttt
     9421 acgttgaacc cgatgatccc cgcccgtatt ctggtctgag agatcaggcc ccttttgggt
     9481 caggctggcg tcccgcccgg gcgaatccgg tccggcgtcc gtcgataacc tgacgccacg
     9541 gcatagaccc tcattgttcg gaggccaccg tggctcgcat attcaccctg gattttctca
     9601 aaaccgaaac ggcgtcaggc gcggcgctcg gcattgcggc cgtcgcggcg ctggtcgtcg
     9661 ccaactcgcc cttggcgggc gagtatttca gctggctgaa gagttatcac gtcctccaga
     9721 tcggaccgct ccggctcgag gagtcgattt ccaactggat caaggaaggc ctgatggcgg
     9781 tcttcttcct cgtggtcggg ctggagatca aatacgaaat cctgcgcggc gaactcagtg
     9841 atccaagaaa actggcgact cccgtgcttg cagcgctggg gggcatggtc gccccggctc
     9901 tggtttacct cgcgatatcg agcgcgaccg gaggtccgca cggcggctgg cccgtgccgc
     9961 tggccactga catcgccttc gccctcgcgg tcttcgccat ggtcggcaag aacctgccgc
    10021 cgtcgctgag ggtgttcctg ctcacgctcg ccatcgtcga cgatctcggc gccatcgcct
    10081 tgatcgctgt gctgttcagt cagggtgtcc acctcatgcc cctgctgggc gtcctcgctc
    10141 tgctggtcac cggcgcgatc tggtcgggag ttcgccgtat ccccgcgccc ttctgggtca
    10201 tggggttcgc tgcggtctgg tatctgacgg ttcaggccgg gatcagcacc tcgcttagcg
    10261 ccgtcgcatt cgccttgatc gtgccgatca agcctcgcac cgaggatcag cagtcgccgc
    10321 tcaaggaggc gatgcatgac ctgcacccgt atgtcgcctg gctggtgctg ccgctgttcg
    10381 ccttcgccaa ggcgggtgtt tcgttcgccg gcctgtcgct ggatcaggcc atggcgccgc
    10441 tggtgcttgg catcgccgcc gggcttttcc tcggcaagca gatcggtgtc ttcggcgcag
    10501 cctggctggc gtcgaagctg aacatcggcg ctcgaccgac agacgccacc tggcttcagg
    10561 tctatggcgt cagcctgctt tgcggtgtgg gtttcaccat gagcctgttt atcggcgctc
    10621 tcgctttccc cggcgcgatc gattcgcctg aacaggtcga ggtcaagatc ggggtacttg
    10681 cggggtctct gctgtcagcc ttcgccggag cgttggccct gaccatcgct aatcttccct
    10741 cgcgcaaggt tttggccaga accggcccgg atactgcata aggtcatccg aatattgacc
    10801 taacgtcatc ggtttttgtc gcttttgcgt cacacggcgt aggctagcag gcggaaaaca
    10861 ggcgaaaagt gggcctcgcg cggtacggct tcttgttgct ttaataagct tgccctacgc
    10921 caagggttcg agattccgcc gtcccatcaa gaggacgcgg ttgggaacag gaaacgccgc
    10981 gactgctccc aagggacagt cgggccatga gccgggagcc gtccatgcgt cttacgcaaa
    11041 ttcttcgcag cactacgtcc gcagccgtcg tcggcgctgc ggttttcggc ttcgccggga
    11101 tcgcatcagc ccagaccgcg gatcaggacg gacccaccgc gaccgtcgac gacatcatcg
    11161 tcaccgccca gaagcgtgaa cagagcctgc aggacgtgcc aatcgtggtc acgtcgctga
    11221 atcaggaagc cctcaacaac gccggcgtga aggacatcaa ggatctccag atcctgacgc
    11281 ccggcatgac cgtcacctcg accacgtcgg aagcctcgac cacggtccgt atccgcggcg
    11341 tcggcaccgt gggtgacaac cccggtctgg aaagctcggt cggcgtcgtc atcgacggcg
    11401 tctatcgctc gcgcaacggc gtcggcttcg gcgatctcgg cgaactggag cggatcgaag
    11461 tcctcaaggg cccgcaaggc acgctgttcg gcaagaacac ctcggccggc gtcgtcaaca
    11521 tcatcaccga agcgccttcg ttcacgcccg gatacaacgc cgaactgacc tggggcaact
    11581 tcaacgctct cggcgcctcg ggttcggtca ccggcccgat caatgaccag gtcgccttcc
    11641 gcctgtacgc cgccaggcgc acgcgagacg gcttcaaccc tgttgatatc ggcgacggcc
    11701 cgcgttcgca gagggatgac cagaaccagg atttctggac cgctcgcggt caggttctga
    11761 tcctgccgaa cgacaacacc tcgatccgca tcatcgccga ctattccaag cgtgacgaat
    11821 actgctgcac gggtacgcag gtccgcacgg gccagaccta tccgttcatc gacggcctct
    11881 cgaccggaac cggccagcgc ccgccgaacg cgacgttcag ccgactgccg ttctcgcgca
    11941 ccggcttcgc caacaacggc accgaacagg agatcgagga ccgcggcatc tcggccgaag
    12001 gaaatttcga cctcacaagc ttcggcggcg cgactctgac caccgttacc tcgtggcgcc
    12061 agtgggactc ggtcaacggc caagacattg actacaccgg cgctgatatc ctgcatcgcg
    12121 acccgaacgg cgactacggc taccgcgtcg gcaacatcac tcaggaagtc cgtctggccg
    12181 gtcagaccga caagctggac tggctcgttg gcgccttcgc cacgcgcgaa cacatcgacc
    12241 gcacagacag ctatatcttc ggccctgact acacgccgtt tctgtctttc ctgctcagcg
    12301 ccaacctgaa tcgcgccgtg ccggctttcc cggtcagccc gaatgacatc ggttgcttca
    12361 cccgcaccgc ccagacggcg ctgggtctgc agcagtgcct cggcaccggc ggcgccgtct
    12421 ccggcggcgg cataggcttc atcactggcg caggcgttcg cgactcttac agccaggatt
    12481 cggactcggt agcgctgttc accaacaaca gcttccacgt gacggacaag ctcgatatca
    12541 ccgtcgggct tcgttacacc tacgactcca agtcgctgga tgcgcttcag acgaaccaga
    12601 acggcaactc caacacctgt gccgctgccg gagccaacgc aggcgccatc gtcggcgtgc
    12661 tgggcgccgc cggtcagacg atcctcggca ccatctgcct gccctgggct aacgccgcct
    12721 tcaataaccg cgccacgcat caggaagaga gcgacggcga gttcagcggc acagtcaagg
    12781 cggcttaccg cttcaacccg tctctgatga cctatgtgtc gtacgcacgc ggctacaaga
    12841 gcttcggtta caatctcgac cgggtgcaga gcggcgttac gccgttgcct tcgaccttct
    12901 tcccgtcgga actggtcgac agctacgaag ccggcttcaa ggcc
//
LOCUS       DASVTM010000270         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_824985,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000270 DASVTM010000000
VERSION     DASVTM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824985"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="kdgD"
                     /locus_tag="VF633_07430"
     CDS             complement(<1..812)
                     /gene="kdgD"
                     /locus_tag="VF633_07430"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HEX8470925.1"
                     /translation="MSRMTPAEMAATIGGGLLSFPVTHFDAAHQFVEKDYREHCAWML
                     EYELAGLFAAGGTGEFFSLTPAEVGKVVKAAVEETNGKTPVVAGCGYGTAIAVDLAQS
                     AEAAGADGLLLLPPYLMVPGQEGLAAHVEAVCKATSLGVIVYNRDNAVLQPETLAKLC
                     DRNPNLVGFKDGVGDIELMMRVYARMGDRLTYVGGLPTAETFAPAYLEMGVTTYSSAI
                     FNFVPEFALSFYKAVRARDHATIMAGLRDFVLPYIALRDQGKGYAVSIVKAG"
     gene            1081..2523
                     /locus_tag="VF633_07435"
     CDS             1081..2523
                     /locus_tag="VF633_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8470926.1"
                     /translation="MTDTLQLSHLINGERVDTARPGESHNPSNTNEIVANTPDGGQAE
                     VDAAVAAAKAAFPAWSEASPEVRSDVLDRAGTLVMERREQLGRLLSREEGKTLPEGIG
                     ETVRAGRILKYFAGEALRVHGQNLASTRPGVEIQTYRQAVGVYGLITPWNFPIAIPAW
                     KAAPALAFGNTVVMKPAGLTPATASALADILVEAGLPKGVFNMVIGRGGVGQAIVDHP
                     DVDGLSFTGSQGVGAGVALGAIKRQARVQLEMGGKNPLIVLDDADLERAVMIALDGSY
                     YATGQRCTASSRLIITDGIHDQFVALLAEKVAALRVGDALDPNTQMGPAVSEAQMETS
                     YKYIEIARTAGGRIVTGGERLSLDNPGWYVQPTLIADTDASAIINTEEVFGPVAATIR
                     VKDYEEALHVANTIEFGLAAGIVTTSLKHARDFQRRAKAGMTMVNLPTAGVDYHVPFG
                     GSNKSSYGAREQGFAAQEFYTQTKTSYSWS"
     gene            2753..3832
                     /locus_tag="VF633_07440"
     CDS             2753..3832
                     /locus_tag="VF633_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX8470927.1"
                     /translation="MIPATKPLQTKGRGARTNASGRFEPVVHENFDDGWTADDEAPAP
                     LRTTLTPEHARTIIAKNTSPDIGFDRSINPYKGCEHGCIYCYARPSHAWMGLSPGLDF
                     ESRIFFKPEGARLLEQELSARKYVCKRIHIGGNTDPYQPVERELKSTRSILQVMQRFN
                     QPFSIITKSNLITRDVDILGPMGREGLATAFVSITTLDRGLARAMEPRASTPQRRLDA
                     VKVLADAGVPVGVGFAPVIPGLNDHELEAVLEASAKAGAMTAMYVTLRLPLEIKDLFR
                     EWLADARPERAARVMSLIRQTRGGKDYDADWSQRMKGVGPVAELIATRFKAALKRYGL
                     EGERHVLDVTKFRVPADARAQLELF"
     gene            complement(3836..>4939)
                     /locus_tag="VF633_07445"
     CDS             complement(3836..>4939)
                     /locus_tag="VF633_07445"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX8470928.1"
                     /translation="EAPEGILDAIVTSLIGLHDLNGEATFRNSRAGSIYIVKPKMHGP
                     QEAGFTNRLFDAVEDLLGLERHTIKVGVMDEERRTSANLAACIHAVKDRIVFINTGFL
                     DRTGDEMHTSMRAGAMVRKAEMKSSDWIASYEDRNVQIGLACGLSGKAQIGKGMWAAP
                     DRMADMLDQKIGHPRSGANTAWVPSPTAAVLHATHYHRVDVFARQAKRAAEPVAALSR
                     LLTIPLARGHNWSPEEVRAELENNAQGILGYVVRWIDQGVGCSKVPDIHDIGLMEDRA
                     TLRISSQHITNWRLHGVCTDAEIDAAFERMAIKVDAQNAGDSLYQPMAGREADSLAYQ
                     AARALVFEGVEQPSGYTEPLLHRFRRRAKGEAI"
BASE COUNT          870 a         1670 c         1573 g          826 t
ORIGIN      
        1 atgcccgcct tcacgatcga cacggcatag cccttacctt ggtcgcgcag ggcgatgtag
       61 ggcaggacga agtcgcgcag accggccatg atggtggcgt gatcgcgggc ccggacggcc
      121 ttatagaagc tcagggcgaa ctcgggcacg aagttgaaga tcgccgacga gtaggtcgtg
      181 acgcccatct caagataggc cggagcgaag gtctcggcgg tcggcaggcc gccgacgtag
      241 gtcaggcgat cccccatccg ggcatagacc cgcatcatca gctcgatgtc gcccacgccg
      301 tccttgaagc cgacgaggtt ggggttgcgg tcgcacagct tggccagcgt ctcgggctga
      361 agcacggcgt tgtcgcggtt gtagacgatg acgcccagcg aggtcgcctt gcagacggct
      421 tcgacgtgcg ccgcgaggcc ttcctggccg ggaaccatca gatagggcgg cagcagcagc
      481 aggccgtcgg cgccggcggc ttcggccgac tgggccagat cgaccgcgat cgccgtacca
      541 tagccgcagc ctgcgacgac cggcgtcttg ccgttggtct cctccacggc ggccttgacc
      601 accttgccga cctcggcagg cgtcagggag aagaactcgc cggtgccgcc ggcggcgaac
      661 aggccggcca gctcatactc cagcatccag gcgcagtgct cacggtagtc cttctcgacg
      721 aactggtgag ccgcatcgaa gtgggtgacc gggaaggaca gcaggccgcc gccgatcgtc
      781 gcagccatct ctgcgggggt cattctggac atggagttcc tcactacaag acggctcctg
      841 ccgcccggcg ctgtgatcgc gccctgatga acgctaacga ccccgtcgat gcgggtctaa
      901 gccaaagggg ctatcgaccg atgcgggatt tggcttgatc acgctgcacc gaggctcaca
      961 gcgaccgatc gatgcgccgc cgctctggat cgatactcca ttggatgttc gcccgggcct
     1021 gtcggcggac tacaccgcgc cccatatagc cgcgacagac agcttcgaag gaacgcgccc
     1081 gtgaccgaca ccctgcagct ctcccacctc atcaacggcg agcgtgtcga tacggcgcgg
     1141 cctggcgaga gccacaaccc gtccaacacc aacgagatcg tcgccaacac ccccgatggc
     1201 ggtcaggccg aggtcgacgc cgccgtcgca gccgccaagg ccgccttccc cgcttggtca
     1261 gaagccagcc ccgaggtccg ctccgatgtg ctcgaccgcg ccgggacgct ggtcatggaa
     1321 cgccgcgaac aactcggtcg cctgctctct cgtgaagagg gcaagaccct gccggaaggc
     1381 atcggcgaga cggtccgcgc cggccgcatc ctcaaatact tcgccggtga ggcactgcgg
     1441 gtgcacggcc agaacctggc ctcgacccgt ccgggcgtcg agatccagac ctatcgtcag
     1501 gccgtcggcg tctatggcct gatcacgccc tggaacttcc ccatcgccat cccggcgtgg
     1561 aaggccgcgc ccgcgctggc gttcggcaac accgtggtga tgaagcccgc cggcctgacg
     1621 cccgcgaccg cttcggccct cgctgacatc cttgtcgagg ccggtctgcc gaagggcgtg
     1681 ttcaacatgg tcatcggacg aggcggcgtc ggacaggcca tcgtcgatca ccccgatgtc
     1741 gacggcctgt ccttcaccgg ctcgcagggc gtcggcgccg gcgtggccct cggcgccatc
     1801 aagcgtcagg cgcgggtaca gctggagatg ggcggcaaga acccgctgat cgtgctcgac
     1861 gacgccgacc ttgagcgcgc cgtcatgatc gcactcgacg gctcctacta tgcgaccggc
     1921 cagcgctgta cggcttccag ccgcctgatc atcaccgacg gcatccacga ccagttcgtc
     1981 gccctgctgg ccgagaaggt cgccgccctg cgcgtcggcg atgctctgga tcccaacacc
     2041 cagatgggtc cggcggtctc cgaggcccag atggagacca gctacaaata catcgagatc
     2101 gcacggactg cgggcgggcg gatcgtcacc ggcggcgagc gcctgagcct cgacaacccg
     2161 ggctggtacg tccagccgac cctgatcgcc gacaccgatg cttcggccat tatcaacacc
     2221 gaggaggtgt ttggccccgt cgcggcgacc atccgcgtca aggactacga agaggcgctc
     2281 cacgtcgcga atacgatcga gttcggcctc gcagcgggca tcgtcaccac ctcgctgaag
     2341 cacgcgcgcg acttccagcg ccgggccaag gctggcatga ccatggtcaa cctgccaacc
     2401 gcaggcgtcg actaccacgt cccgttcggc ggctcgaata agtccagcta tggtgcccgc
     2461 gaacagggct tcgcggcgca ggagttctac acccagacca agaccagcta cagctggagc
     2521 tagcgggcta gccgtcccac cctcgcatca gggcgggagg gccaaggacc acagaaccca
     2581 tctgattgag ccggacggcc tgctgtccgg ctcatttcgt aggcgcaccc ggccgcgcgc
     2641 ctacgtccgg gtttgacgct tccttctcgg ataacatcat gcagctaccg ctcagggcaa
     2701 ctcccgccct tcatgttctt gctttgttct gacatcgtgt tatctctcct gcatgatccc
     2761 ggcgacaaag cccctgcaaa cgaaaggccg cggcgctcgc accaacgcct ctggccggtt
     2821 cgaaccggtc gtccacgaaa acttcgacga cggctggact gccgacgacg aggcccctgc
     2881 ccctttgcgg accacgctca cgcccgagca tgcccgcacc atcatcgcca agaacaccag
     2941 tccggacatc ggtttcgacc ggtccatcaa tccgtacaaa ggatgcgaac atggctgtat
     3001 ctactgttat gcccgcccgt cccatgcctg gatgggccta tccccgggcc tggatttcga
     3061 gagccggatc ttcttcaagc ccgaaggcgc gcgtctcctg gaacaggaac tcagcgcccg
     3121 gaaatacgtc tgcaaacgca tccacatagg cggcaatacc gacccctatc agccggtcga
     3181 gcgcgagctg aaatcgacgc ggtcgatcct gcaggtgatg caacggttca accagccgtt
     3241 ctcgatcatc accaagtcga acctgatcac ccgcgacgtc gacatcctcg gcccgatggg
     3301 gcgcgagggg ctggccacgg cgttcgtttc gatcacgacg ctggatcggg ggctagcccg
     3361 ggccatggag ccgcgcgcgt cgacgccgca acgcaggctt gatgcggtga aggtgctggc
     3421 ggatgccggt gtgccagtcg gggtgggttt cgcaccagtg atcccgggtc tcaatgacca
     3481 cgaactcgaa gccgtcctcg aagcatccgc caaggctggc gcgatgactg ccatgtatgt
     3541 gacgctgcgg ctgccgctgg agatcaagga cctgtttcgc gaatggctgg ccgatgccag
     3601 gcccgaacga gctgcccggg tcatgtccct gatccggcag acgcgtggcg gcaaggacta
     3661 tgacgctgac tggtcccagc ggatgaaggg cgtcggcccc gtcgccgaac tgatcgccac
     3721 ccgcttcaag gccgccctta aacgttatgg ccttgaggga gagcggcacg ttctggacgt
     3781 cacgaagttc cgcgtcccgg cagacgccag ggcgcaactg gagctgtttt agggctcaaa
     3841 ttgcctcgcc cttcgcccgg cggcggaagc gatgcagcaa gggttcggta tagccgctcg
     3901 gctgttcgac gccttcgaac accaaagccc gggcggcttg atacgcgagg ctatcggcct
     3961 cacgtccggc catcggctga tagagcgaat ctcccgcgtt ctgggcgtcc accttaatgg
     4021 ccatgcgctc gaacgcggcg tcgatctcgg cgtcggtgca gacgccgtgc agccgccagt
     4081 tggttatgtg ctgggacgat atacgcaagg tggcgcggtc ttccatcagg ccgatgtcgt
     4141 ggatgtcggg caccttggag cagccgacgc cctgatcgat ccagcgcacg acatagccga
     4201 ggatgccctg ggcgttgttt tcgagctcgg cgcggacctc ctccggcgac cagttatggc
     4261 cgcgggccag cgggatggtc agcaggcgag acaaggcggc gaccggctcg gcggctcgct
     4321 tggcctgacg cgcgaacacg tcgacgcggt gatagtgggt ggcgtgcagg acggcggccg
     4381 tcggcgaagg cacccaggcc gtgttggcgc cggagcgcgg atggccgatc ttctgatcca
     4441 gcatgtcggc catccggtcc ggcgcggccc acatgccctt gccgatctgg gccttccccg
     4501 acaggccgca ggcaaggccg atctggacgt tgcgatcctc gtaggacgcg atccagtcgg
     4561 aggatttcat ctcggccttg cgaaccatgg cgccggctcg catcgaggtg tgcatctcgt
     4621 cgccggtgcg atcgaggaag ccggtgttga tgaagacgat ccggtccttc accgcatgga
     4681 tacaggccgc gagattggcc gaggtacggc gttcctcgtc catcacgccg accttgatcg
     4741 tgtgccgttc cagacccagc aggtcctcga ccgcatcgaa cagccggttc gtgaaacccg
     4801 cctcctgcgg cccgtgcatc ttgggtttga cgatatagat cgagcccgcg cggctgttgc
     4861 ggaacgtcgc ctcgccgttc aggtcatgca gcccgatcag gctggtcacg atggcgtcga
     4921 ggatgccttc gggagcctc
//
LOCUS       DASVTM010000271         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_343150,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000271 DASVTM010000000
VERSION     DASVTM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343150"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..969)
                     /gene="fliP"
                     /locus_tag="VF633_07450"
     CDS             complement(157..969)
                     /gene="fliP"
                     /locus_tag="VF633_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260623.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX8470929.1"
                     /translation="MASSLFTVPTRAELKKAALLSLITTLLCLIWPLAALAQDAAASG
                     SSINVDLGTGAGLTERVVQLVGLMTVLSLAPSIVIMTTSFVRIIVVLSLLRTALGMQQ
                     SPPNAVLVSLALFLSAIVMAPTWQDAYDSGIRPLLDQQMELPQAFDAASEPVKNFMLA
                     QVDRDDLSLFVRLGKVEPNTQAADLPLRVVTPAFMISELKKAFEIGFLLFVPFLVIDL
                     VVSSVLMSMGMMMLPPVVISLPFKLIFFVLVDGWRLVAGSLVESFTRHSVGG"
     gene            complement(972..1343)
                     /locus_tag="VF633_07455"
     CDS             complement(972..1343)
                     /locus_tag="VF633_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX8470930.1"
                     /translation="MNVLDFARAIFGLAFVLGLIGLCAWAARRYAPQLMAKLGAERGA
                     RRLQVMETLVLDPARRLVLVRVDDEERLILLGEGRELIEPRQPAPGPAPVEIAPEPAI
                     PPAAKPFSRPTFTRLNLGGPK"
     gene            1484..1891
                     /gene="flgB"
                     /locus_tag="VF633_07460"
     CDS             1484..1891
                     /gene="flgB"
                     /locus_tag="VF633_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX8470931.1"
                     /translation="MGFTDLPLLGQIKGRLSWLDERQRVVAQNIANSSTPGYVARDLN
                     QPTDFAAAMKAGGALQMTRTSAAHIAPPQTNVARFSSESAPDSETTLDGNSVVLEEQM
                     LKMSESRMAYDAAIGLYSKWMSMLRMAARKPGG"
     gene            1945..2331
                     /gene="flgC"
                     /locus_tag="VF633_07465"
     CDS             1945..2331
                     /gene="flgC"
                     /locus_tag="VF633_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX8470932.1"
                     /translation="MAVASTALRAQQSRMRIIAENIANAESTARTAGGTPYRRQIPVF
                     EARNVDGATGVALAQVQPDQSDFRMDYDPSHPAANADGYVMRPNVDTLVEAMDMREAQ
                     RAYEANLNVIETARSMESRTLDILKR"
BASE COUNT          515 a          784 c          782 g          381 t
ORIGIN      
        1 agcatctttg gcgacggagg cgatcagttc agcttgagtg gtcatgtggt cgtttccaat
       61 aactcgcccg attcagagcg atgaccgggg gatcaacacg cttttaatgc gacttggcaa
      121 gcggcgatga cgcgcaaacg ccctgacagc ggggatttac cccccgactg agtgtctggt
      181 gaagctttcc accaggcttc ctgcgaccag tctccagccg tctacgagca cgaagaagat
      241 cagtttgaac gggagggaaa tcacgacggg aggcagcatc atcataccca tggacatgag
      301 cacgctcgag accaccagat cgatcacaag gaacggaacg aaaagaagaa atccgatttc
      361 aaacgccttc ttgagctcgc tgatcatgaa ggccggagtg accacgcgca ggggcaggtc
      421 agccgcctgg gtattgggct cgaccttgcc cagccgcacg aacagcgaga ggtcgtcccg
      481 gtccacctgg gccagcatga agtttttcac aggttcggac gcggcatcaa acgcctgggg
      541 cagttccatc tgctgatcga gcagggggcg gatgcccgaa tcataggcgt cctgccatgt
      601 cggggccatg acgatggcgc tcaggaacag ggccagcgac accagaacgg cgtttggggg
      661 cgactgctgc atgcccagcg ccgtccgcaa aagcgatagc acgacgatga ttcgcacgaa
      721 gctggtggtc atgatgacga tcgacggcgc cagcgaaagg acggtcatca gaccaaccag
      781 ttgcaccacc cgctcggtca gccccgcacc ggttccaagg tcgacgttga tcgaggatcc
      841 gctagccgcc gcatcctgag ccaatgccgc cagcggccag atcaggcaga gcagggtggt
      901 gatcaacgac agcagcgccg cttttttcaa ctcggcgcgc gtcgggacgg tgaagagaga
      961 agaggccacc ttcatttcgg cccccccagg ttcagcctgg taaaggtcgg gcgtgaaaac
     1021 ggcttggcgg cgggggggat cgcgggctct ggcgcaattt cgaccggggc agggcccggc
     1081 gcaggctgac gcggctcgat cagctcgcgg ccttcgccaa gcaagatcag ccgttcctcg
     1141 tcgtcgacac ggaccaggac cagccgccgg gcagggtcca gcacgagagt ttccatgacc
     1201 tgcagtcgcc gcgctccacg ctcggcgccg agcttggcca taagttgcgg cgcgtaacgc
     1261 cgggccgccc aggcgcaaag cccgatcagg ccaagcacga aggccaggcc gaagatggcg
     1321 cgggcgaaat cgaggacgtt catgcgggcg ctggttccgg ggccgcagaa acgcgcggcc
     1381 gtcagacgac agtcttaggc atcgtggtta acgacccgtt gaaataaccc cctgttaacc
     1441 atgcaggcgg cattttctgc ctatcgaatg ggagagcgcc ccgatgggct tcaccgacct
     1501 gccactgctg ggccagatca agggccgcct gagctggctc gacgagcgcc agcgcgtggt
     1561 cgcccagaac atcgccaact cctcgacgcc cggctacgtc gcacgcgatc tgaaccagcc
     1621 gaccgacttc gcggcggcga tgaaggccgg tggcgctctg cagatgaccc gcacgagcgc
     1681 cgcccatatc gcaccgcctc aaacgaacgt ggcgcgcttc tcgtcggagt ccgcaccgga
     1741 ctcggagacc acgctcgatg gcaactctgt cgttctcgag gagcagatgc tcaagatgtc
     1801 cgagagccgc atggcctatg acgccgccat tggcctctat tcgaaatgga tgtcgatgct
     1861 gcggatggcc gccagaaagc ccggcggctg agaccggatc aggaatagac catgcccgat
     1921 cccgtacccc cccgtaacag cgccatggcc gtggcctcta ccgccctgcg cgcgcaacag
     1981 tcacgcatgc ggatcatcgc ggagaacatc gccaacgcgg agtcaaccgc gcggaccgcc
     2041 ggaggcacgc cctatcgccg tcaaatcccg gtgttcgagg cgcgcaacgt cgatggcgcg
     2101 accggtgtcg ccctggcgca ggttcagccg gaccagagcg attttcgcat ggactacgac
     2161 ccgtctcacc ccgcggccaa cgccgacggc tatgtgatgc gtcccaacgt cgacaccctg
     2221 gtggaagcga tggacatgcg cgaggcgcag cgggcttacg aagccaacct gaacgtcatc
     2281 gagacggcgc ggtctatgga aagccgcacc ctcgatatcc tgaaacgata ggagctagac
     2341 catgaacccg atggcggcga tgaaggccta tgcggcgata cagggcggcg cttcgccgac
     2401 cggcggcgca gccccggcgg cggcgcccgg tttcagcgaa atgctgcaaa acgtcatggg
     2461 cg
//
LOCUS       DASVTM010000272         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_343171,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000272 DASVTM010000000
VERSION     DASVTM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343171"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /gene="mgtE"
                     /locus_tag="VF633_07470"
     CDS             complement(<1..1133)
                     /gene="mgtE"
                     /locus_tag="VF633_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX8470933.1"
                     /translation="MSDKTALLDPDVKRRVIAVEDHEALDEDYVLTPSFVEKVVDASD
                     DGDGLRLRSLLEDLHPADVADLMGFLTSEHRAVVVLWLPPELLADTLPELDEGIREEV
                     LERVPHLTLAEALQELDSDDAAAVVEDLEDDQRERVLSAMPAGDRAAIESSLSYAEDS
                     AGRLMQREVMAAPAFWNVGDTIDHIRRQGDDLPELFFDIYVVDPMNRPIGGLPISRLL
                     RSSRDVLLTDVMEPINDIAVDQDQEEVAYIFEKYHLISAPVIDASGRLVGQITVDDIV
                     NIIQEENREDILRLAGVRDEDRGSSVLEIVQGRVPWLAINLFTAVLGASVIAFFGATI
                     EQIVALAVLMPIVSAIGGNAGTQSLTVTVRALAMRELNNANAWR"
     gene            complement(1229..1313)
                     /locus_tag="VF633_07475"
     tRNA            complement(1229..1313)
                     /locus_tag="VF633_07475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1277..1279),aa:Leu,seq:gag)
     gene            complement(1437..>1664)
                     /locus_tag="VF633_07480"
     CDS             complement(1437..>1664)
                     /locus_tag="VF633_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8470934.1"
                     /translation="AANIAEGYGRATKLSYISFLRIAQGSLKELETHLILATRVGIAA
                     QQPTDDILIQTNEIGRMLHSLMGKLRDHPGR"
BASE COUNT          282 a          495 c          584 g          303 t
ORIGIN      
        1 cgccaggcgt tggcgttgtt cagctctcgc atcgccagcg cacgcaccgt gaccgtcagc
       61 gattgggtgc ccgcgttgcc gccgatggcg gagacgatcg gcatcagcac cgccagcgcc
      121 acgatctgct cgatcgtcgc accgaagaag gcgatgacgc tggcgcccag gacggcggtg
      181 aacaggttga tggccagcca gggcacgcga ccctggacga tctcgagcac cgacgagccc
      241 cggtcctcgt cgcgcacacc ggcgaggcgc aggatgtcct cgcggttctc ttcctgaatg
      301 atgttgacga tgtcatcgac ggtgatctgg ccgaccagac gcccgctggc gtcgatgacc
      361 ggggccgaga tcaggtgata cttctcgaaa atgtaggcga cttcttcctg atcctgatcg
      421 acggcgatgt cgttgatcgg ctccatgaca tcggtcagga gaacgtcgcg gctgctgcgc
      481 agcagacggc tgatcggcag gccgccgatg ggccggttca tcggatcgac gacatagatg
      541 tcgaagaaca gttcgggcag gtcgtcgccc tgacgacgga tgtggtcgat ggtgtcgccg
      601 acgttccaga aggcgggcgc ggccatgacc tcgcgctgca tcaggcggcc ggcggaatcc
      661 tcggcgtagg acagcgagct ctcgatcgcg gcgcggtcgc cggcgggcat ggccgacaga
      721 acgcgctcgc gctggtcgtc ctcgaggtcc tcgaccacgg cggcggcgtc gtcggaatcg
      781 agttcctgca gcgcttcggc cagagtcagg tgggggacgc gttccagcac ctcctcgcgg
      841 atgccctcgt cgagttcggg cagggtgtcg gcaagtagct ccggcgggag ccagagcacg
      901 accacagcgc ggtgctcgga cgtcaggaag cccatgaggt cggcgacgtc ggcggggtga
      961 aggtcctcga gcagcgaccg gagccgcagg ccgtcgccgt cgtcggaggc gtcgaccacc
     1021 ttttcgacga aggacggcgt caggacatag tcctcgtcca gcgcttcgtg gtcttcgacc
     1081 gcgatcacgc ggcgtttgac gtcagggtcg agaagggcgg tcttgtcgct cacgcggttg
     1141 cctccctctc gatgtattcc agccctggta gtggccttct atccctgtat cgtcatgtcc
     1201 tgtcagctcg ccggagcgct ctgactgatg gtgcggtcga gaagactcga acttccacgg
     1261 gttgccccac agcgacctca acgctgcgcg tctaccaatt ccgccacgac cgctcgcatc
     1321 ggagcggggc tgatagcgga ggtgcgccgt gggcgcaaga gaggtgttgg gggttacgga
     1381 agaaggcagg taggcagtag gcagtaggca gtgaagcggg ggcaagccgc ttcagtttag
     1441 cgccccgggt ggtcgcggag cttgcccatc agactgtgaa gcatgcggcc gatctcgttg
     1501 gtctggataa gaatgtcgtc agtgggttgc tgggcggcga tgccgactcg ggtggccagg
     1561 atcaagtgcg tttcaagctc tttcagggag ccttgggcga tgcgcaaaaa gctgatgtag
     1621 gagagtttgg tcgctcggcc gtaaccttcg gcgatattag cggc
//
LOCUS       DASVTM010000273         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_611268,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000273 DASVTM010000000
VERSION     DASVTM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611268"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..937
                     /locus_tag="VF633_07485"
     CDS             104..937
                     /locus_tag="VF633_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8470935.1"
                     /translation="MATRTAVDAGWTPIWTAPSVGALSRGVRDEIGRLRLMLANRIRW
                     SEPTTLTLAAVLGLSLYFTVLPGVDIAVSDLFYRTGDGFFLAQEPVLKALRRSSSYVM
                     GLILLTALVGVVMRTLTRSEKRTVCARRCGFLLAGVALGPGLVVNSWLKASWGRARPV
                     QTDLFGGDAVFSPAWQVSNGCEHNCSFVSGEASSAAWMVAALMLLPPRWRPWAAAPVV
                     TYAVALSLNRLAFGGHYLSDILLSWAITGLVLAVLHRVMVACPIATRLRHASGLAARS
                     C"
     gene            984..3035
                     /locus_tag="VF633_07490"
     CDS             984..3035
                     /locus_tag="VF633_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX8470936.1"
                     /translation="MTDTPPASTIKRIELTVRALILGCLLAVIFTAANTYLGLKVGLT
                     FASAIPAAVISMAVLRAFKGSTIWENMTVQTVASVGGAMSSIIFVLPGLVMVGWWMDF
                     PFWESVAICALGGVLGVTFSIPLRRALVVNANLPYPEGVAAAEVLKVGSPGIEQSDEA
                     VRENKAGLGIVVVGAIISALFAFLISARAFAGEAARFIKLPAFLGGGATGVGFGMQFA
                     LLGAGHLIGLTVGLAQLFGLILAWCIAVPILTSPDTIAWLVAHGIPSIATTLPAGASS
                     EDLAGLVWSREVRFMGAGVIGVAAIWTLIKLLGPLVGGLTSALAAQRRRAGGEVLERT
                     EQDIPIGIVGGLSIIALVGISILLAFFTQGTPLAASAPILVIGGLVYVVVIGFAVAAI
                     CGYMAGLIGSSNSPVSGVGIIAILVASLLMLVVMGISGVPADPSFVAFALMVTAVVFA
                     VAVIANDNLQDLKTGQLVEATPWRQQVALIVGVVAGALVIPFILSQLNGAFGFEGGPK
                     GIADAPLAAPQATLISALAKGVIAGDLRWDLIGIGAVIGVVVIILDAVLEKATKGKIK
                     LPPLAVGIGFYLPAAVTTMLVVGAVAGWLYDKGIAGTRFADIGRRMGVLLASGLIVGE
                     SLFGVFFAGVIVSTGKESPFGLIPEGSGWPAMLAGVVVFVLLAFGLYRWIRGVSAKVA
                     G"
     gene            complement(3197..3706)
                     /locus_tag="VF633_07495"
     CDS             complement(3197..3706)
                     /locus_tag="VF633_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEX8470937.1"
                     /translation="MSDTPAYDRDSLLAWMAENGVAQTTHDHPAVFRVEEGLELKAGM
                     PGVHTKNLFLKDKKQRLWLISAAQDTVIDLKRAPSVIGSDRLSFGNEALVFETLGVRP
                     GSVTALGLINDVDRRVTFVLDRKLHEAAIVNFHPVTNTATTALDQAAFRRFLALLERE
                     PIVVDFDRL"
     gene            3774..4514
                     /locus_tag="VF633_07500"
     CDS             3774..4514
                     /locus_tag="VF633_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEX8470938.1"
                     /translation="MELECYPMTATPPDLVPGRQSRNWMDAFVSRHPYRCLPLTMANT
                     TGWEILCPFGFTAEWNGGPQQSDIKITPDRPQPTLDHFVTSHFSRGVLTMHPQYLFRT
                     PPGWVMVCSGSPNHVKDGIQPLVGLIETDWLPFPFTMNWLFTRPGRVKFEKGEPFCFI
                     TLTEHKKVEQFQPIIRTLESNPVMKGQFDAWNRQRTDFNNRLASGDPDAAKEAWQRFY
                     FKGELPEDLGNAPETHSNKRRLKSPRVG"
BASE COUNT          667 a         1550 c         1510 g          862 t
ORIGIN      
        1 aaaaagaggc cgaaccgcca cgcgacggtg acctttgcga cgcacgacgc ccccagactg
       61 gcccggagat atcgatccgg ggggaacgat ggacgggacg aacatggcga cgcgcacggc
      121 tgtagacgcc ggctggaccc cgatctggac cgctccgtcc gtgggggcgc tgtcacgagg
      181 cgtgcgcgac gagatcgggc ggttgcggct gatgctggcc aaccgcatcc gctggtccga
      241 acccaccact ctgacgctgg ccgccgtgct ggggctctcg ctgtatttca ccgtcctgcc
      301 cggcgtcgac atcgcggtca gcgacctgtt ctaccggacc ggcgacggct ttttcctcgc
      361 tcaggagccg gtgctgaagg cgctgcggcg cagctccagc tatgtcatgg gcctgatcct
      421 gctgacggcg ctggtcggcg tggtgatgcg gaccctgacc cgcagcgaaa agcgcaccgt
      481 ctgcgcccgg cgctgcggct tcctgctggc gggggtcgcg ctgggtccgg gactggtggt
      541 caacagctgg ttgaaggcga gctggggccg cgcccgtccg gtacagacgg acctgtttgg
      601 cggcgatgcg gttttctcgc cagcgtggca ggtgtccaac ggttgcgagc acaactgttc
      661 cttcgtctcg ggcgaagcgt cctcggcggc gtggatggtg gcggcgctga tgctgttgcc
      721 gccccgatgg cgcccgtggg cggccgcgcc ggtcgtgaca tacgccgtcg ccctgtcgct
      781 gaaccggctg gcgttcggcg gccactatct gtcggacatc ctgttatcct gggcgatcac
      841 cggactggtt ctggcggtgc tgcaccgggt gatggtggcc tgtccgatcg cgacgcggct
      901 gaggcatgcg tcgggtctcg ccgcgcgctc ctgttgatcc caagggcgtt ctcggcttac
      961 ctccggcgtt caagagggac gccatgaccg acacaccacc cgcctcaacg atcaagcgga
     1021 tcgaactgac cgtccgcgcc ctcatcctag gctgtctgct ggccgtaatc ttcaccgccg
     1081 ccaacaccta tctgggcctg aaggtcgggc tgacgttcgc ctcggccatc ccggcggcgg
     1141 tcatctccat ggcggtgctg agggcgttca agggctcgac catctgggag aacatgacgg
     1201 tccagaccgt cgcctcggtc ggcggcgcga tgagctcgat catcttcgtc ctgcccggtc
     1261 tggtcatggt cggctggtgg atggacttcc cgttctggga gtcggtggcc atctgcgcgc
     1321 tcggcggcgt gctgggggtg accttctcca tcccgctgcg ccgcgccctc gtggtcaacg
     1381 ccaacctccc ctaccccgag ggcgtcgccg ccgcagaggt gctcaaggtt ggctcgcccg
     1441 gcatcgaaca gagcgacgaa gcggttcgcg aaaacaaggc gggcctcggc atcgtggtcg
     1501 tcggcgcgat catctcggcc ctgttcgcct tcctgatctc ggcccgcgcc tttgcaggcg
     1561 aggccgcgcg gttcatcaag ctgcctgcct ttctcggcgg cggcgccacg ggcgtcggct
     1621 tcggcatgca gttcgccctg ctgggcgccg gccacctgat cggcctgacc gtgggtctgg
     1681 cgcagttgtt cggcctgatc ctggcatggt gcatcgctgt gccgatcctg acgtcaccgg
     1741 acaccatcgc ctggctggtc gcccacggca tcccctcgat cgctacaacg cttcctgccg
     1801 gcgcctcgtc cgaggatctg gccggtctcg tctggtcgcg tgaagtccgc ttcatgggcg
     1861 cgggcgtcat cggcgtcgcc gccatctgga ccctgatcaa gctgctcggc ccgctggtcg
     1921 gcggcctgac ctcggctctg gcggcccagc gccgtcgggc gggcggagaa gtcctcgaac
     1981 gcaccgagca ggacattccg attggcatcg tcggcggcct gtcgatcatc gctctggtcg
     2041 gtatctcgat cctgctggcc ttcttcaccc agggcacgcc gttggcggct tcggctccga
     2101 tcctcgtcat cggcggtctt gtctatgtgg tggtcatcgg cttcgccgtg gcggccatct
     2161 gcggctatat ggccggcttg atcgggtcgt cgaacagccc ggtctccggc gtcggcatca
     2221 tcgccatcct cgtcgcctcg ctgctgatgc tggtggtgat gggtatctcg ggcgtgccag
     2281 cggatccgtc gttcgtcgcc tttgccctga tggtgacggc ggtcgtcttc gccgtcgcgg
     2341 tcatcgccaa cgacaacctt caggacctca agaccggcca gttggtcgag gccacgccat
     2401 ggcgtcagca ggtcgccctg atcgtcggcg tcgtcgccgg cgctctggtc atccccttca
     2461 tcctgagcca gctgaacggc gccttcggct tcgagggcgg ccccaagggc atcgctgacg
     2521 cgccactggc cgcgcctcaa gccacgctga tctccgctct ggccaagggc gtcatcgctg
     2581 gtgatctgcg ctgggacctg atcggcatcg gcgccgtgat cggcgtggtg gtcatcatcc
     2641 tcgacgccgt gctcgagaag gcgaccaagg gcaagatcaa gctgccgccc ctcgccgtcg
     2701 gcatcggctt ctacctgccc gccgccgtga ccacgatgct ggtcgtcggc gccgtggcag
     2761 gctggctcta tgacaagggc atcgccggca cccgcttcgc ggacatcggc cgccggatgg
     2821 gcgtgctgct ggcgtcaggc ctgatcgtgg gcgagagcct gttcggcgtg ttcttcgccg
     2881 gggttatcgt cagcaccggc aaggagtcgc cgttcggcct gatccccgaa ggctcaggct
     2941 ggcccgcaat gctcgcgggc gtggtggtgt tcgtgctgct ggcgttcgga ctgtaccgct
     3001 ggatccgtgg ggtctccgcc aaggtcgctg ggtaggtctc taccctctcc cggcgggaga
     3061 gggcttgagc ctccgggagc gaagcgatcg gtcaggcgaa agggtgaggg gttgctgtgt
     3121 ccgagcggac gcgccaacct ctcaccctcc cacttcgcta cgctcgcggg tccctccctc
     3181 tcccgccggg agagagtcaa agccgatcga agtccaccac tatcggctcg cgctccagca
     3241 gcgccagaaa ccgccggaac gccgcctgat ccagcgccgt ggtcgccgta ttggtcacgg
     3301 ggtgaaagtt gacgatcgcc gcctcgtgca gcttgcggtc cagcacgaag gtcacgcgtc
     3361 ggtcgacatc gttgatcagt cccagcgcgg tcaccgagcc cggccgcacg cccagcgtct
     3421 cgaacaccaa cgcctcgttc ccgaacgaca gcctgtccga gccgatcacc gacggcgctc
     3481 gcttcagatc gatgaccgtg tcctgagccg cagagatcag ccacagccgc tgcttcttgt
     3541 ccttgaggaa caggttcttg gtatggacgc cgggcatccc ggccttgagt tccagaccct
     3601 cctcgacccg gaacaccgcc ggatggtcgt gggtcgtttg ggcgacgccg ttctccgcca
     3661 tccaggcgag caaactatcg cggtcgtaag ctggcgtatc agacatggtt gaggtgaccg
     3721 gctcgcacgg gttaggagtg gcgtccgtcc cgactacaga ctggagcccg gccttggaac
     3781 tggaatgcta tccgatgacg gcgacgccgc cggacctcgt gcccgggcgt cagtcgcgta
     3841 actggatgga cgccttcgtc agccgccacc cctatcgctg cctgccgctg accatggcca
     3901 acacgacggg gtgggaaatc ctgtgcccgt tcggcttcac cgccgagtgg aacggcgggc
     3961 cacagcagtc ggacatcaag atcacgcccg accggccaca gccgacgctg gatcatttcg
     4021 tgacctcgca cttctcgcgc ggggtcctga ccatgcaccc gcagtatctg ttccgcacac
     4081 cgccgggctg ggtgatggtc tgctcgggtt cgcccaacca tgtgaaggac ggcatccagc
     4141 cgctggtcgg cctgatcgag accgactggc tgccctttcc cttcacgatg aactggctct
     4201 tcacccgccc cggccgggtc aagttcgaga agggcgagcc cttctgtttc atcaccctga
     4261 ccgagcacaa aaaggtcgag cagttccagc cgatcatccg gacgctggag tccaatccgg
     4321 tgatgaaggg tcagttcgac gcctggaacc gccagcgcac cgacttcaac aatcgcctcg
     4381 cctcaggtga ccccgacgcg gccaaggagg cttggcagcg gttctacttc aagggcgaac
     4441 tgcccgaaga tctcggcaat gcgcccgaga cccattcgaa caagcggcgg ctgaagtcgc
     4501 cgagggtggg gtaaggaggc agtaggcagg taggcaatag gcagtaggag aaagcggttc
     4561 gggctgcccc tactgcctat tgcctactg
//
LOCUS       DASVTM010000274         7026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_825284,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000274 DASVTM010000000
VERSION     DASVTM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825284"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VF633_07505"
     CDS             <1..894
                     /locus_tag="VF633_07505"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8470939.1"
                     /translation="VLFGGLMTNSPERLWPELLWDVRAELGEGPVWDATRACVWFVDI
                     KGRKLHRYGVEDQTQASWDTPDQTGFALPAEDGSLICGVRGGLHRFTPETGEFALIQP
                     VETDRPGNRLNDGFVAPDGTLWFGSMDDAEEAETGALYRWRRGELTRMDDGYGITNGP
                     ALSPDGLTMYHHDTAHRTVFAFDHADGEITNRRVFAVPTKGYPDGPSVDSAGVIHVGL
                     FHGGGVARFSPEGAELDPIAFPVPTVTKVVFGGDDLRTLFCTTAWKGQDAATRAAYPT
                     MGGLFAVRVETPGLPQALVAL"
     gene            891..1721
                     /locus_tag="VF633_07510"
     CDS             891..1721
                     /locus_tag="VF633_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX8470940.1"
                     /translation="MITLRHGYWLATVALQGGAVASLAKAGRDVLRPAPEGATDPLDF
                     ACFPLVPYANRVAGGRFAFAGREVRLPVQPQFAPHALHGEGWLGLWAILEQTDTRLVL
                     SFAGDDWPWAYEARQTLTLSDDGLTIDLVLINRDERPMPAGLGLHPYFPRFPSSWLDA
                     STTGVWTGQDIVPDRLAPSNVVADWTDRSLTDATFVDHCFDGWTGPARLSDDGGTTIV
                     RASADRLHVYVPTGETYVCLEPVTHRPDVFNQASPGEQGFVVLQPGESLSLSMTVGRD
                     "
     gene            complement(1744..3549)
                     /locus_tag="VF633_07515"
     CDS             complement(1744..3549)
                     /locus_tag="VF633_07515"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX8470941.1"
                     /translation="MTQSTPKPFRPLRSRAWFDNPDNPDMTALYLERYLNYGLTLEEL
                     RSGKPIIGIAQTGSDIAPCNRHHIELAKRVREGIREAGGIAMEFPVHPIQETGKRPTA
                     GLDRNLAYMGLVELLYGYPLDGVVLTIGCDKTTPACLMAAATVDIPAICLSVGPMLNG
                     WLKGERIGSGTIIWKAREMMARGELDYEGFIDLVATSAPSVGYCNTMGTASTMNSLAE
                     ALGMSLPGSAAIPAPYRERPQIAYKTGLRIVEMVHEDLKPSDILTADAFHNAIVVNSA
                     IGGSTNAPVHISALARHSGVDLPIKTWETTGHAVPLLVNLQPAGTYLGEDFHQAGGVP
                     AVINQLMGQGLIREDALTVNGKTLGENCSGAEIILPDVIRSFDQPLMTDAGFLVLGGN
                     LFESAIMKTSVISPEFRERYLANPEDPEAFEGPAVVFDGPEDYHHRIDDRATGITERS
                     VLFMRGAGPIGYPGAAEVVNMRPPSYLIEKGVLALACIGDGRQSGTSGSPSILNASPE
                     AAAGGNLALLQTGDIVRVDLKTRRVDTRVSDAEMAERRVALEAAGGFQYPPSQTPWQE
                     LQRANVGQLQTGAVLEPAIKYHRIIDKFGNPRDNH"
     gene            3699..4469
                     /locus_tag="VF633_07520"
     CDS             3699..4469
                     /locus_tag="VF633_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX8470942.1"
                     /translation="MARRSIPDQQRGQDNQHQRGDLALSGHAWIVRALGEDILSGRFP
                     PGDKLPSEQALLDRFGVSRTSLREALKTLTAKGLLVVKTRVGTKVSERIRWNLFDPEV
                     LAWKVSTGMDADFRRHLAEVRRAVEPATAALASLHRTTEDLVTMRQAIDAMREETASE
                     YGFARADLLLHLAIGAASKNPMLRSVASVIETALLEAFTLSPPTRAPDLHRETVDAHA
                     RIVDRIEARDGAGAAAAMLAVIDAGYSRVESEHRSTRG"
     gene            complement(4466..6109)
                     /locus_tag="VF633_07525"
     CDS             complement(4466..6109)
                     /locus_tag="VF633_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX8470943.1"
                     /translation="MKLTRRGAFALVAVGAATPVLAQAPTSPSRALPPGAPLSGDAAR
                     SFGDWTAMTWPKGIEGQRLADLGDGTFLNPVLAGDHPDPSILKDGDTYYMTHSSFESV
                     PGLTIWQSKDLVNWTPIVAALTKNIGSVWAPELCKHDGRFFLYIPARFPDSRTNWVIW
                     ADKIEGPWSEPVDLHLPDHIDPGHVVGEDGKRYLFLSGGDMVQLTDDGMATVGAVRHV
                     YNPWRYPVEWDVEGFSPEGPKVMKRGDWFYMITAVGGTAGPPTGHMVIVARSKSVFGP
                     WEDHPRNPIVRTTTSREKWWSRGHATFVEGPAGDWWTVYHGYENAFWTLGRQTLLDKV
                     VWATDGWPDVVGGDLSHPMAKPANLPGQQHGMALSDDFSTDKFGTQWSFYNPGPDEGR
                     RFRREGGVLHMAAKGTAPKDCSPLTFVQGEHAYQFEVEIEIDEGTTAGLVLFYDFGLY
                     AGLGFDEKRFVTHQYGMERGKPGHTMGRRMHMRVTNDNHIVTFHTSPDGQTWTKFDRQ
                     MEVSGYHQNVRGGFMMLRPGLYSAGAGEARFRGFKYRAL"
     gene            6415..>7026
                     /locus_tag="VF633_07530"
     CDS             6415..>7026
                     /locus_tag="VF633_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX8470944.1"
                     /translation="MDRRAFLTACGIGAGGVLLPGFAGRAIAAAELGDTLDVTIKKRL
                     ADAGLSAATRAGASYCDVRVGRYLRQFVMTREASVENVVNTESTGIGVRVIADGAWGF
                     AATNELTLEAVAGAARLATAIAKANATSQTAPVQLAPTPGVGDRAWRTPIRRNGMTVP
                     IEEKIELLMGVNSAAMGAGASFVASRMFLVNEQKYFASTDGSYI"
BASE COUNT         1193 a         2245 c         2313 g         1275 t
ORIGIN      
        1 gtcctattcg gcgggttgat gaccaatagt cctgagcgtc tttggcctga acttctgtgg
       61 gatgtccgcg ccgaactcgg cgagggtccc gtgtgggatg ccacgcgcgc ctgcgtctgg
      121 ttcgtcgata tcaaggggcg caagctccac cggtacgggg tcgaagatca gacgcaggca
      181 tcgtgggaca cgccggatca aactggcttc gctctgccgg ctgaggacgg ttcgctgatc
      241 tgcggcgtga ggggtggcct gcatcggttc acgccggaga cgggtgagtt cgccctgata
      301 cagccggtcg agactgacag gccgggcaat cgtctgaacg acggcttcgt cgcccctgac
      361 ggaaccctgt ggttcgggtc catggacgac gcggaggaag ccgaaaccgg cgccctctat
      421 cgatggcggc gtggcgagct gacccggatg gacgatggct acggcatcac caatggtccg
      481 gcgctgagcc ctgatggcct gacgatgtat caccacgaca ccgcgcatcg aaccgtcttc
      541 gccttcgatc acgccgacgg cgagatcacc aaccgtcggg tcttcgctgt gcccaccaaa
      601 ggctatccgg acggtccgtc ggtcgacagc gcgggcgtga tccatgtcgg cctgttccac
      661 ggcggcggcg tcgcccggtt ttcccctgaa ggcgccgagc tggacccgat cgcctttcct
      721 gttccgaccg tgaccaaggt tgtgttcggc ggggacgatc tcaggaccct cttttgcacc
      781 acggcatgga agggacagga tgcggcgacg cgggcggcgt atccgacaat gggcgggctg
      841 ttcgccgttc gggtcgagac acctggactg cctcaagctc tggtcgccct ttgatcaccc
      901 tgaggcatgg ctactggttg gcgaccgtcg cccttcaggg cggagctgtc gcctcgttgg
      961 ccaaggccgg acgcgacgtg cttcgaccag cgcctgaagg cgcgaccgat ccgctggact
     1021 tcgcctgctt tccgctggtc ccgtatgcca accgggtggc cggggggcgg ttcgccttcg
     1081 ctggccgtga ggtcaggctg ccggtccagc ctcagtttgc cccccatgcc cttcacggcg
     1141 aaggctggct ggggctgtgg gcgatcctcg aacagaccga cacccgcctc gtcctgagct
     1201 tcgcagggga cgactggcca tgggcttacg aagcgcgcca gacgctgacc ctgtcggacg
     1261 acggcctgac gatcgacctc gttctgatca atcgcgacga gcgaccgatg cctgcgggtc
     1321 ttggcctgca tccctatttt ccgcgctttc cgagcagttg gctcgacgct tcgaccaccg
     1381 gagtgtggac ggggcaggat atcgtgccgg acagactcgc cccgtcgaac gtcgtcgcag
     1441 actggaccga tcgttcgctg acggacgcta cttttgtgga ccattgcttc gacggctgga
     1501 ccggcccggc gcgactgtcg gacgacggtg ggaccaccat cgtccgcgct tcagccgatc
     1561 gcctgcacgt ctatgtgccg accggcgaaa cctacgtctg ccttgagccg gtgacccacc
     1621 gccctgacgt attcaatcag gcgtcgccgg gagagcaggg cttcgttgtc ctgcaacccg
     1681 gcgagtcgct gtcgctctca atgacggtcg gccgcgacta gcctcttcgt tcgccgacgg
     1741 cccctagtgg ttgtcgcggg ggttgccgaa cttgtcgatg atccggtgat atttgatcgc
     1801 gggctccagc accgcgccgg tctggagttg cccgacgttg gcccgttgca gttcctgcca
     1861 gggcgtctgc gacggcggat actggaagcc gcccgcagcc tccagcgcca cgcgccgctc
     1921 ggccatctcg gcgtcggaga ccctggtgtc gacccgccgc gtcttcagat cgacacgtac
     1981 gatatcgcct gtttgcagca gggcgagatt gccgccggcc gccgcctcgg gcgatgcgtt
     2041 caggatcgag ggtgagccag aggtgccgga ttgccgcccg tcgccgatgc aggcgagcgc
     2101 cagcacgccc ttctcgatca gataggacgg cggtcgcatg ttcacgacct cggccgcgcc
     2161 gggatagcca atcggtccgg cgccgcgcat gaacaggacg ctgcgttcgg tgatgccggt
     2221 cgcgcggtcg tcgatgcggt ggtggtagtc ttcgggacca tcgaagacga cggcgggacc
     2281 ttcgaaggct tcgggatcct ccgggttggc cagataccgt tcgcggaatt cgggcgagat
     2341 cacgctcgtc ttcatgatgg cgctctcgaa caggttgccg ccaagcacga ggaagcctgc
     2401 gtccgtcatc aggggctgat cgaacgagcg gatgacgtca ggcaggatga tctcggcccc
     2461 ggagcagttt tcgcccagcg tcttgccatt cacggtcagg gcgtcctcgc ggatcagccc
     2521 ttggcccatg agctggttga tgaccgcagg aacgccgccg gcctgatgga agtcctcgcc
     2581 gagataggtc ccggccggtt gcagattgac cagcagcggc acggcatggc ccgtcgtctc
     2641 ccaggtcttg atcggcaggt cgacgcccga gtgacgcgcc aaggccgaga tgtggaccgg
     2701 cgcattggtc gaaccgccga tggccgagtt gaccacgatg gcgttgtgga aggcgtcggc
     2761 ggtcaggatg tcggacggct taaggtcctc gtgcaccatc tcgacgatgc gaagcccggt
     2821 cttgtaggcg atctggggac gctcgcggta gggggcagga atcgccgccg agccgggcag
     2881 ggacatgccc agcgcctcgg ccagcgagtt catcgtcgag gccgtgccca tggtgttgca
     2941 atagcccacc gacggcgccg aagtagccac gaggtcgatg aagccttcat agtccaactc
     3001 gccacgcgcc atcatctcgc gggccttcca gattatggtg cccgagccga tccgttcgcc
     3061 tttcagccaa ccgttcagca tcggcccgac cgacaggcag atggcgggga tgtccacggt
     3121 cgccgccgcc atcagacagg caggggtggt cttgtcgcag ccgatggtca gcacgacccc
     3181 gtccagagga tagccgtaaa gcaactcgac cagacccata taggcgaggt tgcggtccag
     3241 accggccgtc ggtcgcttgc cggtttcctg gatcggatga accggaaact ccatggcgat
     3301 gccgccggcc tcgcggatgc cttcgcgaac gcgcttggcc agttcgatat ggtggcggtt
     3361 gcacggcgcg atgtccgagc cggtctgggc gatgcctatg atcggctttc cggagcggag
     3421 ctcttccagc gtcaggccgt agttcagata ccgctccaga tagagcgcgg tcatgtccgg
     3481 gttgtccgga ttatcgaacc aggcgcgcga acggagaggc ctgaagggct tcggtgtcga
     3541 ttgggtcaaa cggcaagtcc tgcgccgaaa cggcgcgtaa aattgagaga gtcaagatat
     3601 tgggccgagg gaaatcgtgg tgtgtctgag gcctatgccc gcttcccacg aattccaggc
     3661 tttgccgtgg gggcgggccg cgtgtaagtc cgacccggat ggctagacga tcgatcccgg
     3721 accagcagag ggggcaggac aaccagcacc aacgcggcga tctcgcgctg tccggccatg
     3781 cctggatcgt tcgcgctctt ggcgaggaca tcctgtcggg ccggtttccg ccgggcgaca
     3841 agctcccctc cgaacaggcg ctgctcgatc gcttcggggt gtcccggacg tctctgcgcg
     3901 aagctctcaa gacgctgaca gccaaaggcc tgctggtggt caagacgcgc gtcggcacca
     3961 aggtctcgga gcgcattcgc tggaacctgt tcgacccgga agtcctcgcc tggaaagtca
     4021 gcaccgggat ggatgccgac ttccgccgtc atctggccga ggtccgccgc gcggtcgagc
     4081 ctgcgacggc cgcgttggcg tcgctgcatc ggacgaccga ggatctggtg acgatgcgcc
     4141 aggccatcga cgccatgcgc gaggagaccg cgtccgaata cggtttcgct cgcgctgacc
     4201 ttttgctcca tctggctatc ggcgcggcgt cgaagaaccc gatgctgcgt tccgtcgcca
     4261 gcgtgatcga gacggccctc ctcgaagcct tcaccctgag cccgccgacc cgggcgcccg
     4321 acctccatcg cgagaccgtc gacgcccacg cgcggatcgt cgaccgtatc gaggcgcgcg
     4381 acggcgccgg cgccgcggct gcaatgctcg cggttatcga cgccggttat agtcgtgtgg
     4441 agtccgagca ccgttcgacc cggggctaga gggcgcggta cttgaagcca cggaagcgcg
     4501 cctctcctgc gcccgccgaa tacagccccg gtcgcagcat catgaagccg ccgcgaacgt
     4561 tctggtgata gccggagacc tccatctgac gatcgaactt ggtccatgtc tggccgtcag
     4621 ggctcgtatg gaaggtgacg atgtggttgt cgttcgtaac ccgcatatgc atgcgccgac
     4681 ccatcgtatg gcccggcttt ccgcgctcca tcccgtactg gtgagtgaca aagcgcttct
     4741 cgtcaaagcc gagtccggcg tagaggccga agtcgtagaa caggaccaga ccggcggtcg
     4801 tgccctcgtc gatctcgatc tcgacctcga actgataggc gtgttcgccc tgaacgaagg
     4861 tcagcggcga acagtccttc ggcgcggttc cctttgccgc catgtgcagc acgccgcctt
     4921 cgcgacggaa ccggcgtccc tcgtccggac ccggattgta gaaactccac tgggtgccga
     4981 atttgtcggt cgagaagtcg tccgacagag ccatgccgtg ctgctggccc ggcaggttcg
     5041 caggcttggc catcgggtga gacaggtctc cgccgaccac gtccggccag ccgtcggtgg
     5101 cccagacgac cttgtccaga agcgtctggc gacccagcgt ccagaaggcg ttttcgtagc
     5161 cgtgatagac cgtccaccag tctcccgcag gaccttcgac gaaggtcgca tggccgcgcg
     5221 accaccattt ctcacgcgaa gtcgtggtgc ggacgatcgg attgcgcgga tgatcttccc
     5281 acggtccgaa gacggatttc gagcgcgcga cgatgaccat atgtcccgtc ggcggacctg
     5341 ccgtgccgcc gactgcggtg atcatgtaga accagtcgcc gcgcttcatc accttggggc
     5401 cttcgggcga gaagccctcg acgtcccatt cgaccggata gcgccacggg ttatagacgt
     5461 gccgaactgc gccgaccgtg gccatgccgt cgtctgtcag ttgcaccatg tcgccgccac
     5521 tgagaaacag atagcgtttg ccgtcttcgc cgacgacgtg gccgggatcg atgtggtcgg
     5581 gcaggtgcag gtcgaccggc tcgctccatg ggccttcgat cttgtcggcc cagatgaccc
     5641 agttggttcg gctgtcgggg aagcgagccg ggatgtagag gaagaaccgg ccgtcatgct
     5701 tgcacagctc gggcgcccag accgagccga tgttcttcgt cagcgccgct acgatgggcg
     5761 tccagttcac cagatccttc gactgccaga tcgtcaggcc cggcaccgac tcgaacgacg
     5821 agtgggtcat gtaataggtg tcgccgtcct tcaggatcga cggatcggga tggtcaccgg
     5881 cgaggactgg attaaggaag gtgccgtcgc cgagatcggc cagtcgttga ccttcgatgc
     5941 ccttgggcca tgtcatagcg gtccagtcgc caaacgaccg cgcggcgtca cccgagagcg
     6001 gggcgccagg aggaagggcg cgagacggtg aggttggagc ctgcgctaga accggagtcg
     6061 ccgcacccac tgcgaccagg gcgaatgcac cgcgtctcgt cagcttcatg gtcatttcct
     6121 cacccgatcg tatttgtatt ataaatcatc ctaacgccgt gctggaccgt ggcaagaggg
     6181 agacccgaag aacgtctcgc ggcctgccga tatgccttac gagcgccaat gagcatgccc
     6241 gcaccccggc cgcgagatcg atggagccga cagccaggct gatcctaccc accgaagaaa
     6301 tcctgttttg cgagcgccat atgtttgacc gctgagacgt cagcagcgaa tgtagggggc
     6361 tctccggatc aagcacgatg tcgaccggca gcagatatcg ccaggagaga agacttggac
     6421 agacgcgcgt tcctgaccgc ctgcggcatc ggtgcaggcg gggtcctgct cccggggttt
     6481 gccggccgcg ccattgccgc tgcggaactg ggcgacacac tggacgtgac gatcaagaag
     6541 cgattggccg acgcagggct cagcgccgcc acacgggccg gcgccagcta ctgcgacgtg
     6601 cgggtcgggc gctatctgcg ccagttcgta atgacgcggg aagccagtgt cgaaaacgtc
     6661 gtcaacaccg aatcgaccgg cataggcgta cgggtgattg cggatggcgc atggggcttc
     6721 gctgcaacca acgagttgac cctggaggcg gtcgcgggcg cggctcggct cgcaaccgca
     6781 atcgccaagg cgaatgccac cagccagacc gctcctgttc agttggcccc tacccccggc
     6841 gtcggcgatc gggcctggcg caccccgatt cggcgcaatg gcatgaccgt gccgatcgag
     6901 gagaagatcg agctgctgat gggcgtgaac tcggctgcca tgggggccgg agccagcttc
     6961 gtcgcctccc gtatgttcct cgtcaacgag caaaaatatt tcgcctctac cgacggctct
     7021 tacatc
//
LOCUS       DASVTM010000275         3273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_557869,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000275 DASVTM010000000
VERSION     DASVTM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557869"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2647)
                     /locus_tag="VF633_07535"
     CDS             complement(<1..2647)
                     /locus_tag="VF633_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8470945.1"
                     /translation="MSKSNIRPVAKGRVVRLLQGCAVVALIGAPVQAMAQQTRTPPAE
                     EATTVDEVVVTGSFATSLADALTIKRRADSISDSVSASDIGNFPAVNVAEALQRVPGV
                     SISREAGEGQFVSVRGLGPNFQSVTLNGSPIAYNENIRNSDQSGRQFQFRVIPADLIS
                     AIVVTKAPTADLLDGGIGANIDIRTADALETKPFVTARAFTHYENRTEAHTPNGSISA
                     GWRNADRTFGLIAGVSLATRDVQFDRLQTTGYTNRVINGVTVAVPNEIQLTLEREERT
                     RISAMGGLTWKPTDAFALRVDALYSSFNNVIDEDRTSFSFGGRADFTTRFDATSARVV
                     NGVLYAGKINGGRVDRNAEYSEQQHDNFSIKAQADWDIAGWTITPSLSYSEAVSKLPV
                     PLQRISGGTIENPVGLTYSYDFGDDPVGNREYASVLTNLDLGSPTATTVTGYRTRPIN
                     SEDTDKTALLNIQRDLGARFGGLELGTFRFGGQYTERSRDYQRRDRVLALRPGVNVNT
                     APFLSEQLPTNAFNQSIRNLYAQGLTYNRGLFGGSYVIPGESDDTNAQSDDLVATGAD
                     LQQSYGVDETVTAAYGRTDFSASVGPFPLTGNLGLRWVSTDTTVKGTRLTAGRNAANA
                     VTTIVTPQTFEGSYDEFLPSLNLNFELRENVLFRLGASRTLTRPSLADLRTAVVPNSS
                     LITQVFLTGQTALDTATASAKVGVGGNPELRPYTAVNFDASLEWYFNDFGALYGAVFK
                     KDISDFIGGIGRTEQILFATQAGQTLTADLLITRPQNIGDATVEGFELGFSYKLDFGL
                     GLSASATFTDSSAEIEATPGVITTAGLQGVSDQSFSISPFFERGPFEVHFSYTYRSDF
                     TANGNITPGSNAVLDENSAIVADGFG"
     gene            complement(2772..2951)
                     /locus_tag="VF633_07540"
     CDS             complement(2772..2951)
                     /locus_tag="VF633_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470946.1"
                     /translation="MKRRRIDFYTYSSLLARYAGRNGEETFRVAARYSEFELDFPPQL
                     LDRPPDLPHAPLMKF"
     gene            complement(3090..>3273)
                     /locus_tag="VF633_07545"
     CDS             complement(3090..>3273)
                     /locus_tag="VF633_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470947.1"
                     /translation="LPGAGLMLGLGLAVERAFRLSATDKGYWLAIWLMASSPRAWRWR
                     CNSLRQDRQWQLHPDT"
BASE COUNT          586 a          945 c         1144 g          598 t
ORIGIN      
        1 agccaaagcc gtcggcgacg atggccgagt tctcgtcgag cacagcgttg gatcccggag
       61 tgatgttgcc gttggcggtg aagtccgagc ggtaggtata gctgaagtga acctcgaacg
      121 gaccacgctc gaagaacggg ctgatcgaga agctttgatc agatacgccc tgcaagccag
      181 ccgtagtgat cacgcccggc gtcgcctcga tctccgccga cgagtcggtg aacgtcgcgc
      241 ttgccgaaag gcccagaccg aagtccagct tgtaggagaa gcccagttcg aagccttcga
      301 cggtggcgtc gccgatgttc tggggacggg tgatcagcag gtcagcggtc aacgtctggc
      361 cggcttgcgt cgcgaacagg atttgctcgg tccgtccgat gccgccgatg aagtcagaaa
      421 tgtccttctt gaacactgcg ccgtacaggg cgccgaagtc gttgaagtac cattccagcg
      481 aggcgtcgaa gttgacggca gtgtagggac gcagttcagg gttgccaccg acgccgacct
      541 tcgcgctggc tgtggcggtg tcgagcgcgg tctgaccggt caggaacacc tgggtaatca
      601 gcgagctgtt gggcaccacg gcggtgcgca gatcggcgag cgacggacgt gtgagcgtcc
      661 gcgaggcgcc cagacggaac aggacgtttt cacgcagttc gaagttcagg ttcaggctgg
      721 gcaggaactc gtcgtacgag ccttcgaacg tctggggcgt gacgatcgtg gtcaccgcat
      781 tcgccgcgtt gcgaccagcg gtcaggcggg tgcccttgac ggtagtgtcg gtcgataccc
      841 aacgcaggcc gaggttaccg gtcagcggga acggaccaac agaggcgctg aagtcggtgc
      901 gaccgtaggc ggcggtgacg gtctcgtcga cgccgtagga ctgctgcagg tcggcaccgg
      961 tggcgaccag atcgtccgac tgggcgttgg tgtcatccga ctcgccggga atgacatacg
     1021 aaccgccgaa caggccgcga ttataggtca ggccttgagc gtacaggttg cggatcgact
     1081 ggttgaaggc gttggtcggc agttgttcgc tcaggaacgg cgccgtgttg acgttcacgc
     1141 cgggacgtag cgccaggacc cggtcgcgac gctgatagtc acgcgagcgt tcggtgtact
     1201 gaccaccgaa acggaacgtg ccgagttcca gtcccccgaa cctcgctccc agatcgcgct
     1261 ggatgttcag aagggcggtc ttgtccgtgt cttcggagtt gatcgggcgc gtacgataac
     1321 cggtgacggt cgtcgccgta gggctgccga ggtcgaggtt cgtcaggacc gaggcgtatt
     1381 cgcgattacc gacgggatcg tcgccgaaat catagctgta ggtcaggccg accgggtttt
     1441 cgatcgtgcc gccgctgatg cgctggagcg gcaccggcag tttcgagacg gcttccgaat
     1501 agctaagcga cggtgtgatg gtccagccgg cgatgtccca gtcggcctgg gccttgatcg
     1561 agaaattgtc gtgctgctgc tcagaatatt cggcgttgcg atcgacgcga ccgccgttga
     1621 tcttgccagc atacaggacg ccattcacga cacgagcgct cgtggcgtcg aagcgggtgg
     1681 tgaagtcagc gcgaccgccg aacgagaagc tggtccggtc ctcgtcgatg acgttgttga
     1741 agctcgagta cagggcatcg acgcgtagcg cgaaggcgtc tgtgggtttc caggtcaggc
     1801 cgcccatggc gctgatacgg gtgcgttctt cacgctcgag cgtcagctgg atttcgttcg
     1861 ggactgcgac ggtcacgccg ttgatgacgc ggtttgtgta gccggtggtc tgcaagcggt
     1921 cgaactgcac gtcgcgggtc gccagcgaca cgccggcgat gaggccgaag gtgcgatccg
     1981 cgttgcgcca tccggcagaa atcgaaccgt tgggcgtatg cgcctcggtg cggttctcat
     2041 agtgggtaaa ggcgcgggcg gtgacgaagg gcttggtttc aagcgcgtcg gcagtgcgga
     2101 tgtcgatgtt ggcgccgata cccccgtcca gcaggtcagc ggtgggggcc ttggtcacaa
     2161 cgatggccga gatcaggtcg gcagggatga cccggaactg gaactggcga ccgctctggt
     2221 cggagttgcg gatgttttcg ttataggcga tcggcgaacc gttcagggtc accgactgga
     2281 agttcgggcc gaggccacga acgctgacga actggccttc accagcttcg cgggagatcg
     2341 acacgccggg tacgcgttgc agcgcctcgg caacgttcac agcggggaag ttgccgatgt
     2401 cggaggccga caccgagtcc gagatcgaat ccgcgcggcg cttgatggtc agggcgtcgg
     2461 caaggctggt ggcgaaagag ccggtgacga cgacctcgtc gacggtggtg gcttcctccg
     2521 cgggcggggt ccgggtctgt tgcgccatgg cctggaccgg agcaccgatg agggcgacga
     2581 cggcgcaacc ttgcagcaga cgaaccacgc ggcctttcgc gaccgggcgt atgttggatt
     2641 tggacatttg atgatctccg caggtcgaat gatcggcgga cgcgtgaaga ccgtctggtc
     2701 cgccgcgtgc accccctgcg gccttcaacg gcggcgatcg ccgtgccctc agcggcgccc
     2761 tgcgggtttc attaaaactt catcagcggg gcgtgaggca ggtctggggg ccggtccaga
     2821 agctgtggtg ggaagtcgag ctcgaactcg gaataccggg cggcgacacg gaacgtctct
     2881 tctccgttac ggcctgcgta gcgggccagc agggacgagt aggtgtaaaa gtcgatcctt
     2941 ctccgcttca catcgaactc gtgaactgga gccagccgcc gcccccgttg gccgatcccg
     3001 cttgcccatc cgcgtggtaa caacgcgtca gggcgcgtcc caagcttggc gcgacgcggg
     3061 aggtaattgt cccaggttcg ccaccaagct tacgtgtcag ggtggagttg ccactggcga
     3121 tcttgccgca acgagttgca gcgccagcgc caggcccgtg gggacgacgc catcagccag
     3181 atcgccagcc agtagccctt gtcggtggcg gacaggcgga acgctcgctc gacagccagt
     3241 cccagaccca acatcagccc tgcgcccggc aag
//
LOCUS       DASVTM010000276         7518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_26854, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000276 DASVTM010000000
VERSION     DASVTM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26854"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VF633_07550"
     CDS             <1..335
                     /locus_tag="VF633_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8470948.1"
                     /translation="DRVQFQQAIVNLVRNAAEAARDRRDGRVVVIGRARSGTAFELRI
                     EDNGPGLEPEELERVFRPLTTTKTSGMGLGLSVTRTIIERHGGSLFVERSAMGGAAFV
                     FELQRAEL"
     gene            complement(439..588)
                     /gene="cydX"
                     /locus_tag="VF633_07555"
     CDS             complement(439..588)
                     /gene="cydX"
                     /locus_tag="VF633_07555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEX8470949.1"
                     /translation="MWYFTWVLGLGLAVAFGVLNGVWHEFHLFDEGDQGLSEPEAELP
                     ETHTV"
     gene            complement(610..1758)
                     /gene="cydB"
                     /locus_tag="VF633_07560"
     CDS             complement(610..1758)
                     /gene="cydB"
                     /locus_tag="VF633_07560"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX8470950.1"
                     /translation="MDIPLDYATLRMIWWALLGVLLIAFAVTDGFDLGVGALLPFVAK
                     TDEERRMVINTVGATWEGNQVWFIVGGAAIFAAWPFVYAVSFSGFYLAMFLVLAALIL
                     RPVAFKYRSKRPHGAWRTFWDWALFTSGFVPALVFGVAVGNVLLGAPFRLDSDLRSFY
                     EGHLLGLFTPFALICGLLSVSMLVLHGACWLGLKAERGHIAERARRFGTIAGLLSLIL
                     FAAGGLYVAFGPLGFRITGPLDVQGFSNPLRTTVAAAPGAWLDNYGRYPWLMIAPFLG
                     FTGTAAALIGLWRRVEPLAFGGSSAATAGIISTVGLSMFPFILPSSIDPQSSLTAWNS
                     SSSHLTLFIMLVVTAIFLPLILIYTTWVYRVLWGRSTTAALHTNPDLY"
     gene            complement(1782..3383)
                     /locus_tag="VF633_07565"
     CDS             complement(1782..3383)
                     /locus_tag="VF633_07565"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX8470951.1"
                     /translation="MDLAVVDLSRLQFALTALYHFLFVPLTLGLAFMLVIMESIYVMT
                     RRPIWRTITRFWGSLFGINFVLGVATGLTMEFQFGMNWSYYSHYVGDIFGAPLAIEGL
                     MAFFLEATFVGLMFFGWDKLSRVGHLFVTFMVALGTNLSALWILVANGWMQNPVGSAF
                     NPDTMRMEVTDFMAVLMNPVAQAKFVHTVSAGYVIASVFVLGISAFYLLKGRHRAFAR
                     RSMTVASAFGLAASLSVVVLGDESGYALTDNQKMKLAAIEAMWDTEAAPAGLNVFGMP
                     DMKSHTTRFGVQVPWVLGLISTRSLDGKVEGILPLVALAKTRIESGVLAYDALERIKT
                     NPADPVARGDFELHRYDLGYGLMLKRVVADPRQATPQQISATAWDTVPNVPVMFWTFR
                     FMAAIGFAMIGLFATAFVLCTLRKHDTRWFLRLAVIAMPLPWIAAELGWIVAEFGRQP
                     WAVEGVLPTFLGSSSLSVPQLLTTIVGFTVLYGALAVVEVGLLLHAIKKGPFAQQEEF
                     ADRPAPGAPEPRQPLVPPIVAPAVA"
     gene            3580..5202
                     /gene="cydD"
                     /locus_tag="VF633_07570"
     CDS             3580..5202
                     /gene="cydD"
                     /locus_tag="VF633_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEX8470952.1"
                     /translation="MTATVTAEITPASWLKQIGTPWRRSLRITGLLVVADVVPAIGFA
                     AGLALTLASLQGGDAFGSTFWLGLALSGFSLIVRGLLGQRATRMGALAAMAIKGNVRR
                     SLARQLFGARVSEERLTTAVAEGVGALDGYYARFDPTRFAAAATPLIVIAVCALASPV
                     SAAILILTLIPFVIGMVLAGTAAGAESRRQFESLERLSGLFLDRVRALPVLLAFQAEG
                     RTVNDIARGSDDLARRTGRVLKVAFLSSAVLEFFSALSVALVAVYCGFNLLRLLPFPV
                     PETLDLARAIFILALAPEVYAPMRRLAAAYHDRQAAEAAAFSLMTSETVVPSVAASPR
                     IWLKPPAICFQNVAIAYETSSPVIAGFDLDVSAGSMVAIVGPSGSGKTSLLRLLLGLA
                     PLSEGDVEIGDLRLSQIGSLSGAVGWAGQHPMVIPGSLGDNLALVARAASGHQLLEAA
                     GRAGLHGDLDRSLDERGGGLSGGERRRLGLARALLSPSRILVLDEPTANLDAASEADL
                     IPVLREAARGRTTLIATHSAAVWNIADHVVRL"
     gene            5199..6848
                     /locus_tag="VF633_07575"
     CDS             5199..6848
                     /locus_tag="VF633_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8470953.1"
                     /translation="MSGRTRLEALVLAETRRERARLSGAAVCAALVALAAVGLLGLSG
                     WFITSAALAGLAGAAAAQTFNYLLPSAFIRLLAIMRTAGRYGERVWGHEAALKALARL
                     RPALFDGLAALPALKALALSGGEASARLGQDVDALLTLFVRRSAPWGAVIGALTAVGM
                     ASMAGLSAAGILAGAIILSVAGAAFLSRALAAPVGREVQIAQGRLKDRIGALQASRAE
                     LRAYGLETWAAGEVEIRGAELDERQVEAFTAAGWIAAYQTAVAGLAVVAVVLSVTAAP
                     LPLVALAALATVIGLETAQGLANAMIQRGASVEAMTRLSDLLADTPQSRGLQPVGSNI
                     AMADGGLHLTAPCRLAVTGASGFGKTTLIERLMALRSAPAGEQFVGGVDVANADVQAL
                     RGMFAYAAQDVRLMSGTVRENLRLADPKASDADLQRALVDADLWDRIAADPAGLGLRL
                     SENGGGLSGGERRRLTLARAYLRDAPWLVLDEPTEGLDAATEQRVLAGLARHLARTHQ
                     GLILISHRAAPRALCDLVLTVSGLSSDGRVQMAPDQRAEAA"
     gene            complement(6858..>7518)
                     /locus_tag="VF633_07580"
     CDS             complement(6858..>7518)
                     /locus_tag="VF633_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8470954.1"
                     /translation="TLSVCGSLDDADLARLDSLAEHITLGAGDVLTRQGDPANHVFNI
                     TSGSVRVFKLLADGRRQITGFLFAGDFLGLASADSYVFSAEAIEAATACRFRRTEYRA
                     LIRDRPTLETALLDRATHELAAAQNQMVLLGRKTALERVASFLLDLPAQDPARPGPAD
                     HIRLPMTRSEIADYLGLTIETVSRVLTRLKTEGVIRLVSLNEILVERPERLQALAGGD
                     A"
BASE COUNT         1331 a         2413 c         2530 g         1244 t
ORIGIN      
        1 ccgatcgggt ccagttccag caagcgatcg tcaacctcgt gcgcaacgcc gcagaagccg
       61 cgcgagaccg gcgcgacggc cgggtcgtgg tgatcgggcg ggcgagatcc gggacagcgt
      121 ttgagttgag gatcgaagac aacggccccg gcctcgaacc agaagagctt gagagggtgt
      181 tccggccact tacgacgaca aaaacctcag gcatgggcct ggggctctcc gtgacccgga
      241 ccatcattga gcgccatgga ggatccctgt tcgttgagcg aagcgccatg ggcggagccg
      301 ccttcgtctt cgaacttcag cgcgcggaac tctaatcggg gtccaggcac cgggcaacct
      361 gacacgcgcg ccactggtcg gatcgcgcga tcggcctctc cgacgccgcc ttgatccagc
      421 gcagaccgat tatcgccctc acacagtatg cgtttccgga agttcggctt ccggctcaga
      481 caggccctga tcgccctcgt caaagaggtg gaactcgtgc cagacgccgt tcaagacgcc
      541 gaaggcgacg gctaggccga ggcccagaac ccaggtgaaa taccacatga tgtttgctcc
      601 tcagggcgat caatagaggt cgggattggt gtgtagcgct gcggtcgtgg agcggcccca
      661 gagcacgcgg tagacccagg tcgtgtagat caggatcagc ggcaggaaga tcgccgtcac
      721 caccagcatg atgaacaggg tcaagtggct ggatgatgag ttccaggccg taaggctgga
      781 ctgcgggtcg atcgagctcg gcaggatgaa ggggaacatc gacaggccga ccgtcgagat
      841 gatccctgca gtggcggccg aagatccccc gaaggctaga ggctcgaccc gccgccacag
      901 tccgatcaag gccgccgccg tgcccgtgaa accgaggaag ggcgcgatca tcagccaagg
      961 gtagcgaccg tagttgtcga gccacgcacc gggcgcggct gcgacagtgg tgcgcagcgg
     1021 gttcgagaaa ccctgcacgt ccaaggggcc ggtaatgcgg aaacccagag gtccgaatgc
     1081 gacgtaaagg ccccctgcag cgaacaggat caggctcagc aaaccggcaa tggtcccgaa
     1141 gcggcgcgca cgttcggcga tgtgaccgcg ttcggccttg agccccaacc agcaggcgcc
     1201 gtgcagcacc agcatcgaca ccgacaacaa accacagatc agcgcgaagg gggtgaacag
     1261 acccagcaga tgaccttcat agaaggagcg cagatcgcta tcgagccgga aaggcgcacc
     1321 gagcaggacg ttgccgacgg ccacgccgaa caccagcgca gggacgaaac cgctggtgaa
     1381 cagggcccag tcccagaagg ttctccaggc gccgtgcggc ctcttggagc gatacttgaa
     1441 ggcgaccggc cgcaggatca gggccgccag caccaggaac atggcgaggt agaagcctga
     1501 gaagctgacc gcatagacga agggccaggc tgcaaagatc gcggcaccgc cgacgatgaa
     1561 ccagacctga ttgccttccc aggtcgcgcc gacggtgttg atcaccatcc ggcgctcttc
     1621 atcggttttg gcgacgaacg gcaacaatgc gccgacgccc agatcgaagc cgtcggtgac
     1681 ggcgaaggcg atcagcagca cgcccagcag ggcccaccag atcattcgga gggtggcgta
     1741 gtcgagggga atgtccatct cggttttcct aaagggtcag gtcaggcgac tgcgggcgcg
     1801 acaatgggcg gaacgagagg ctggcggggt tcaggcgcgc cgggtgcggg gcgatccgcg
     1861 aactcttcct gctgggcgaa cggccccttc ttgatcgcat gaagcagcag cccgacctcg
     1921 accacggcaa gcgcgccata gaggacggtg aatcccacga tggtggtcaa cagctgcggc
     1981 acgctgaggc tggaggagcc gaggaaggtc ggcaagaccc cctcaacggc ccagggctgg
     2041 cgcccaaact cggcgacgat ccagcccagt tccgccgcga tccacggcag gggcatggcg
     2101 atcaccgcaa gacgcaggaa ccaccgcgta tcgtgtttgc gcagggtgca cagaacgaag
     2161 gccgtggcga acagaccgat catcgcaaac ccgatcgcgg ccatgaagcg gaaggtccag
     2221 aacatcaccg gcacattggg cacagtgtcc catgccgtgg cgctgatctg ctgcggagtc
     2281 gcctgacgag gatcggcgac cacacgcttc agcatcagtc cgtagccgag gtcgtagcga
     2341 tggagctcga aatcgccccg cgccacggga tcggcaggat tcgtcttgat ccgctcaagg
     2401 gcgtcatagg cgaggacacc ggactcgatc cgggtcttgg ccagagcgac cagcggcagg
     2461 atgccttcga ccttgccgtc cagactgcga gtcgagatca ggcccagcac ccacggcacc
     2521 tgcacgccga agcgggtcgt gtggctcttc atgtccggca tgccgaagac attcagcccg
     2581 gcaggcgcgg cctcggtgtc ccacatggcc tcgatggccg cgagcttcat tttctggttg
     2641 tcggtcaggg cgtagccgct ctcatcgccg agcacgacga ccgacagcga ggccgccaga
     2701 ccgaaggcgg aggcgaccgt catcgaccgt ctggcgaacg cgcgatgtct gcccttgagc
     2761 aggtagaaag cggaaatccc gagcacgaag accgaggcga tcacataacc ggcgctgacc
     2821 gtgtgcacga acttggcctg cgccaccggg ttcatcagca cggccatgaa gtcggtgacc
     2881 tccatccgca tggtgtcggg gttgaaggcc gagccgaccg ggttctgcat ccagccgttg
     2941 gcgaccagaa tccacagcgc cgacaggttg gtgccgagcg cgaccatgaa ggtgacgaac
     3001 agatgcccga cccggctgag cttgtcccag ccgaagaaca tcaagcccac gaacgtcgcc
     3061 tcgaggaaga aggccatcag cccctcgata gccagcgggg cgccgaaaat gtcgcctacg
     3121 tagtggctgt agtacgacca gttcatgccg aactggaact ccatggtcag gccggtagcg
     3181 acgccgagca cgaaattgat gccgaacagg cttccccaga accgggtgat ggtccgccag
     3241 atcggacggc gggtcatcac atagatcgac tccatgatga cgagcatgaa ggccagaccg
     3301 agcgtcagcg gcacgaacag gaaatggtac agggccgtca gcgcgaactg cagccgggag
     3361 aggtcgacga ccgccaggtc aatcatgaag ggggctccgc tcagccggtc ccgacgaccg
     3421 acgtttcact cgccctagaa gtcaggcggg acgctcgcct tgatctcgat caaagcggcg
     3481 ccgtacgcgc atccctattg gacggttcgt cctcggaccg ccggtccaac ggcgttttgt
     3541 ccggttgccc gctgtgcttt ccggcgctgg agttggttga tgaccgcgac tgtgactgcc
     3601 gagataacgc ctgcaagctg gctgaagcag atcggaacgc catggcgtcg gtcgctgcgg
     3661 attacgggtc tgctcgtggt ggccgacgtc gttccggcga tcgggttcgc tgcaggtcta
     3721 gccctcacgc tggcctccct tcaaggcggc gatgccttcg gaagcacgtt ctggctgggc
     3781 ctcgcgctca gcggcttcag cctgattgtt cgcggcctgc tgggtcagcg cgcgacccgg
     3841 atgggcgcac tggcggcgat ggcgatcaag gggaatgtac gccgttctct ggctcggcaa
     3901 ctgttcggcg cgagggtttc ggaggagcgt ctgacgaccg ccgtcgccga gggcgtaggc
     3961 gcactggacg gttattatgc gcgcttcgat ccgacgcggt tcgccgccgc cgcgacgcct
     4021 ctgatcgtca tcgccgtctg cgccttggcc agcccggtgt ccgcggccat cctgatcctg
     4081 accctgatcc cctttgtcat tggcatggtg ctggccggca cggcggccgg cgcagaaagc
     4141 cgtcgtcagt tcgagtcgct cgagcggctg tccggcctgt tcctcgaccg ggtccgcgcc
     4201 ctgcccgtcc tgctggcgtt tcaggcggag ggccgcacgg tcaacgacat cgcaaggggc
     4261 tcggacgatc tcgcgcggcg taccgggcga gtgctcaaag tcgctttcct gtcttcggca
     4321 gtgctcgagt tcttctccgc cctgtcggtc gcgctggtcg ccgtctattg cggcttcaac
     4381 ctgctgaggc tcctgccctt tccggttccc gagacgctgg acctcgcgcg cgccatcttc
     4441 attttggcgc ttgctccgga ggtctatgct cccatgcgca gactggccgc cgcctaccac
     4501 gatcggcagg ccgccgaagc cgctgcgttc agcctgatga cgagcgaaac ggtagtgccg
     4561 tcagtcgccg cttcgccaag gatctggctc aagccgcccg ctatctgctt tcagaatgtc
     4621 gccatcgcct atgagactag ctcgcccgtg atcgcgggat ttgatctaga cgtgtctgct
     4681 ggatccatgg tcgcgatcgt cggccccagc ggttcaggaa agaccagtct gctgcgcctg
     4741 ttactgggcc ttgctccctt gagcgaggga gacgtcgaga tcggcgacct gcgcctttca
     4801 cagatcggct cgctgtcggg cgccgtgggt tgggcgggcc agcacccgat ggtcatcccc
     4861 ggatcactcg gagacaatct ggctctggtg gcccgggcgg cctccggaca ccagcttctc
     4921 gaggcggcag ggcgggccgg tctgcatggg gatctggatc gttcgctcga tgagcgtggc
     4981 ggcggcctgt ccggcggcga acgacggcgg ttgggccttg cgagggcgtt gctcagcccc
     5041 agccggattc tggttctcga cgagccgacg gcaaacctgg acgcagcatc ggaagcggac
     5101 ctgatcccgg ttctgcggga agcggcgcgc ggtcgcacca ccctgatcgc gacccattcg
     5161 gctgctgtgt ggaacatcgc cgaccatgtg gtgcggctat gagcgggcgc acacgcctcg
     5221 aggcgctggt cctcgccgaa acacgacggg agcgcgcgcg cctcagtggc gccgccgtct
     5281 gcgccgcgct tgtcgctctt gccgccgtcg gtctgctggg actgtcgggc tggttcatca
     5341 cctcggcggc tttggcgggg ctggccggtg cggctgcggc ccagaccttc aactatctgc
     5401 tgccaagcgc cttcatccgg ctgcttgcca tcatgcgcac ggcgggccgt tacggcgaac
     5461 gtgtctgggg tcacgaggcg gccctgaagg ctctggcccg actgcgaccc gcgctgttcg
     5521 atggcctcgc cgccttgcct gcactcaaag cgttggcgct ttcgggaggc gaggcctcgg
     5581 cccggctggg tcaggacgtc gacgccctgc tgacgctgtt cgttcgtcgg tctgcgccat
     5641 ggggggctgt gatcggagcc ctcacggcag taggcatggc gtccatggcg ggtctctctg
     5701 ctgcaggcat tctggccggg gcgataatcc tgtccgtggc gggcgccgcc ttcctgtcac
     5761 gcgcgctcgc tgcacccgtc gggcgcgaag ttcagatcgc tcaaggccgg ttgaaagacc
     5821 gtatcggcgc cctgcaggct tcaagagcgg agttgcgcgc ctatggcctc gaaacctggg
     5881 cggccgggga ggtcgaaatc cgaggcgccg aactggacga acggcaagtc gaggctttta
     5941 cggcggccgg atggatcgcg gcctaccaga cggcagtcgc cggtctggcc gtcgtcgcgg
     6001 tcgtcctgag cgtcaccgcc gcaccgcttc cccttgttgc gctagccgcg ctcgcaacgg
     6061 tcatcggtct ggaaaccgca caaggactgg ccaacgccat gatccagcgc ggggcctctg
     6121 tcgaagcgat gacccggctt tcggacctgc ttgcagacac accccaatcc agaggcctgc
     6181 agcccgtcgg ttcgaacatc gccatggccg acggcgggct ccatctaacc gcaccctgtc
     6241 ggctcgccgt cacaggcgcg tcgggcttcg ggaaaaccac tctcattgaa cggctgatgg
     6301 cgctgcgttc agcgcccgcc ggcgaacagt tcgtcggcgg cgtggatgtg gccaacgccg
     6361 acgttcaggc gctgcgcggg atgttcgcgt atgccgcaca ggacgtcagg ctgatgtcgg
     6421 gcacggtgcg tgagaacctg cgcctcgccg accccaaggc aagcgacgcc gacctgcaac
     6481 gggctctggt ggacgcagac ctgtgggatc ggatcgccgc cgatcctgcc gggctgggct
     6541 tacgtctgag cgagaacggc ggaggcctgt ccggcggtga aaggcgacgg ctgacgctgg
     6601 ctcgggccta tctgcgagat gcgccgtggc tcgtgctgga tgaaccgacc gagggcctgg
     6661 atgcggcgac ggagcagcgg gtgttggcgg gtctggctcg ccatctggct cgaacccatc
     6721 aaggcctgat cctgatcagt caccgtgcag ccccgcgcgc gctgtgcgat ctggtgctta
     6781 cggtgtcagg cctcagctcc gacggacggg tccaaatggc gccggaccaa cgcgccgagg
     6841 cggcttgatc gccgcaatca ggcgtcgccg cccgccagcg cttgcagccg ctccgggcgt
     6901 tcgacgagga tctcgttcag cgacacgagc cgaatgacgc cctcggtctt cagtcgtgtc
     6961 aacacgcggc tgacggtctc gatcgtcagg cccagatagt cggcgatttc ggatcgggtc
     7021 atcggcaggc ggatatggtc ggccggaccg ggtcgtgccg ggtcctgcgc cggcaggtcg
     7081 agcaggaagg acgcgacccg ctccagcgcc gtcttgcgac ccagcagcac catctggttt
     7141 tgcgcggcgg ccagctcatg ggtggcgcgg tccagcaaag cggtctccag tgtcggccgg
     7201 tcgcggatca gcgcgcggta ttcggtccgt cggaaccggc aggcggtcgc agcctcgatc
     7261 gcctcggccg aaaacacata ggaatcggcg ctggccagac cgagaaagtc gcctgcgaac
     7321 aggaagccgg tgatctggcg gcggccgtcc gcgagcagct tgaacacccg cactgagccg
     7381 gacgtgatgt tgaacacgtg gttcgccggg tctccttgac gtgtgagcac gtcgccggcc
     7441 ccgagggtga tatgttccgc cagtgaatcg agccgggcga ggtccgcatc gtcgagcgac
     7501 ccacaaacgc tcagggtc
//
LOCUS       DASVTM010000277         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_344056,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000277 DASVTM010000000
VERSION     DASVTM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344056"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..636)
                     /gene="nusB"
                     /locus_tag="VF633_07585"
     CDS             complement(106..636)
                     /gene="nusB"
                     /locus_tag="VF633_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX8470955.1"
                     /translation="MSEPNSIQAVLQQLAEAEKERAEPNLTSRQRRARTVARLAAVQA
                     LYQMELAGEGAETVISEFSNHRFDSDIEGEPLAEADEEWFADIVRGVIESQREIDAAV
                     KARLASNWRLERLDATLRALLRSGAWELAHKPEVPREIVIDEYVELAKAFFDEADAKF
                     VNAALDGVARDVRPKA"
     gene            complement(633..1097)
                     /gene="ribH"
                     /locus_tag="VF633_07590"
     CDS             complement(633..1097)
                     /gene="ribH"
                     /locus_tag="VF633_07590"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX8470956.1"
                     /translation="MTDPIRVLIVEARYYDELADVLLDGAKDALRSQGVEFDVVTVPG
                     ALEVPTAIALASEAGKYPTAPRYDGFVALGCIIRGETYHFEIVSDQSAAGLMQLGLKG
                     LAIGNGILTTEDEDQAWARAKMSEGDKGGGAARAALDLIALRKRLRHGLAGE"
     gene            complement(1094..2239)
                     /gene="ribB"
                     /locus_tag="VF633_07595"
     CDS             complement(1094..2239)
                     /gene="ribB"
                     /locus_tag="VF633_07595"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX8470957.1"
                     /translation="MINTIQLSADMFSSPISPIEDILEDARNGRPYILVDAEDRENEG
                     DVIIPAQFATPDQINFMARHARGLICLAITAQRARQLRLPPMAAENRESMTTAFTVSI
                     EAAEGVTTGISAADRARTVQVASDPSRGADDIVSPGHVFPLVARDGGVLVRTGHTEAA
                     VDISRMAGLTPAGVICEIMNEDGTMARMPDLVAFAQLHGLKIGTIADLIAYRRRTERY
                     VERVMDQPFESVHGGPFRLMLYRDTIEGGEHVALVHGRIEQGKPTVVRMHQVDFAADL
                     LGHREDRQNYIPTALKQISDSDEPGVVVFLRDPSLQSLAERLAGAEKPAGNDRAIKAY
                     GLGAQILLDLGVRDMIVMSSTRPEPTALEGYGLRIVGWRGMNGEDQS"
     gene            complement(2239..2829)
                     /locus_tag="VF633_07600"
     CDS             complement(2239..2829)
                     /locus_tag="VF633_07600"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX8470958.1"
                     /translation="MFTGIVTDIGRVRAVTETNRDRRYEIETSWDVSGIDMGASISHA
                     GCCLTVVEKADGWFAVEVSDETLDKTTLGGWKTGDPINLERAAKLGDEMGGHVVSGHV
                     DGLGTVVSIGAVGGSHKVVVEAPAPLHRYIAAKGSITVDGVSLTVNAVDRQTFDLNII
                     PHTWDATTLGRLQPGDAVNLEIDMLARYLARWQETA"
     gene            complement(2831..3466)
                     /locus_tag="VF633_07605"
     CDS             complement(2831..3466)
                     /locus_tag="VF633_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX8470959.1"
                     /translation="MRPRITLKIATSLDGRIATSTGESQWITGPEARLQGHRLRAAGD
                     AILVGVETVLADDPRLTVRLPDGETGADPLRVVLDSRLRTPSFARLAKAGTLIITTRE
                     PQAIGQAEVVRVAGDAQNRPSIDAVLELLAERGVKSLMIEGGGRVAAAFVTAGVVDAI
                     DWFRAPILLGGDGRPGVAALSLGRLANAPKFRRLAVEPLGDDLWERYERVS"
     gene            complement(3466..>3631)
                     /locus_tag="VF633_07610"
     CDS             complement(3466..>3631)
                     /locus_tag="VF633_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX8470960.1"
                     /translation="GETEIPSSVVGDFIMKALKSVDEVAYVRYASVYKDFRHTGDFAR
                     FLGDEGLDES"
BASE COUNT          593 a         1212 c         1211 g          615 t
ORIGIN      
        1 ggcgacatcg tccaccagcc ccctgcccca ctcgggatgg gccaggggcg ccaccagttt
       61 ctggatcgtt tcgaactcgc cggacacgtc gacggcggcc atggcttacg ccttcgggcg
      121 aacgtcgcgg gcgacgccgt cgagggcggc gttgacgaac ttggcgtctg cctcgtcgaa
      181 gaaggctttc gccagctcga catattcgtc gatgacgatc tcgcgcggca cttccggctt
      241 gtgagccagc tcccaggcgc ccgagcgcaa cagggcgcgc agcgtggcgt cgagacgctc
      301 gagacgccag ttggaggcca gacgcgcctt cacggcggcg tcgatctcac gctgcgattc
      361 gatcacgcca cggacgatgt cggcgaacca ttcctcgtcg gcctcggcca agggttcacc
      421 ctcgatgtcg ctgtcgaagc ggtggttcga gaactcggag atgaccgttt ccgcgccctc
      481 gccggccagt tccatctggt acagcgcctg gacagcggca agacgcgcga ccgtgcgcgc
      541 acggcgctgg cgcgaggtca gattgggctc agcccgctct ttttcggcct cggccaattg
      601 ttgcaggacg gcctggatgc tgttcggttc gctcattcgc ccgccagacc atgccggaga
      661 cgtttgcgca aggcgatcag gtcgagagct gcacgtgcgg cgcccccgcc cttgtcgcct
      721 tcgctcatct tcgcacgggc ccaggcctga tcctcgtcct cggtggtcag gatgccgttg
      781 ccgatcgcca gacccttgag gccgagctgc atcaggcccg ccgccgactg atccgagacg
      841 atctcgaaat ggtaggtctc gccacggata atgcagccga gcgcgacgaa gccgtcatag
      901 cgcggcgccg tcgggtattt gccggcttcg ctagccagag caatggcggt cggaacctcg
      961 agcgcaccgg gcacggtgac gacgtcgaac tccacgcctt gcgagcgcag ggcgtccttc
     1021 gctccgtcaa gcaggacgtc ggccagttcg tcataatagc gcgcctcgac gatgaggacg
     1081 cggatgggat cggtcaggat tggtcttctc cgttcatgcc gcgccagccg acgatacgca
     1141 ggccgtagcc ttcgagcgcg gtcggttcgg ggcgggtcga gctcataacg atcatgtcgc
     1201 gcacgccgag atccagcagg atctgggcgc cgagcccata ggccttgatc gcgcggtcat
     1261 tgcccgcagg cttctcagcg ccagccagac gctcggccag gctctgcagc gacggatcac
     1321 gcaggaacac gaccacgccg ggttcgtcgc tgtcgctgat ctgcttcagc gcggtgggga
     1381 tatagttctg gcggtcctcg cgatggccca gcaggtcagc ggcgaaatca acctggtgca
     1441 tgcgcacgac ggtcggcttg ccctgctcga tccggccgtg aaccaaggcg acgtgctcgc
     1501 cgccctcgat ggtgtcgcgg tacagcatca ggcggaacgg cccgccgtgg acgctctcga
     1561 acggctgatc catgacccgc tcgacatagc gttcggtgcg gcggcgatag gcgatcaggt
     1621 cggcgatcgt gccgatcttc aggccgtgaa gctgggcgaa agcgaccaga tccggcatcc
     1681 gggccatggt gccgtcttcg ttcatgatct cgcagatcac gcccgccggg gtcagacccg
     1741 ccatgcgact gatgtccacg gcggcctcgg tatgacccgt gcggaccagc acgccgccgt
     1801 cacgggcgac cagcgggaag acgtggccgg gcgagacgat gtcgtcggcg ccgcgcgaag
     1861 gatcggaggc gacctgaacg gtacgcgccc gatcggcggc cgatatgccg gtagtaacgc
     1921 cctcggcggc ctcgatcgac accgtgaagg ccgtcgtcat gctctcgcgg ttctcggcgg
     1981 ccatgggcgg cagccgaagc tgacgcgcac gctgggcagt gatggcaagg cagatgaggc
     2041 cacgcgcgtg gcgggccatg aagttgatct ggtccggcgt cgcgaattgg gcggggatga
     2101 tgacgtcgcc ctcgttctcg cgatcctcag cgtccacgag gatgtaaggg cggccgttgc
     2161 gggcgtcctc aagaatgtcc tcaatcggac tgatcgggct ggagaacatg tccgccgaga
     2221 gctggattgt attgatcatc acgcggtctc ttgccagcgg gcgaggtagc gcgccagcat
     2281 gtcgatctcg aggttcacgg cgtcgcccgg ctgcagtcgg cccagcgtcg tcgcgtccca
     2341 ggtgtgggga atgatgttga ggtcgaaggt ctggcggtcc acggcgttga ccgtcagcga
     2401 cacgccatcg accgtgatcg agcccttggc ggcgatgtag cgatgcaacg gagcgggcgc
     2461 ctcgacgacg accttgtggg agccgccgac cgccccgatc gacacgacgg tgccgagccc
     2521 gtcgacatgg cccgaaacca cgtgcccgcc catctcgtcg cccagttttg ccgcgcgctc
     2581 caggttgatc gggtcgcccg tcttccagcc gcccagcgtc gtcttgtcga gtgtttcgtc
     2641 cgagacttcg accgcgaacc agccatccgc tttttcgacg accgtcaagc agcagcccgc
     2701 gtggctgatc gatgcgccca tgtcgatccc cgacacgtcc cacgacgtct cgatctcgta
     2761 gcggcgatcg cgattggtct cggtgaccgc gcggacgcgg cctatgtcgg tgacgatgcc
     2821 ggtgaacatg tcaggaaact ctctcgtagc gttcccacag gtcgtcaccc agaggctcca
     2881 ccgcaaggcg ccggaacttt ggagcattgg cgagacgccc aagagacagg gcagcgacgc
     2941 ccggacgacc gtctccgccc agcaggatcg gcgcgcggaa ccagtcgatg gcgtctacga
     3001 caccggcggt cacgaaggcg gcggcgacgc gccctcctcc ctcgatcatc aggcttttca
     3061 cgccgcgctc ggccagcagt tcgagcaccg cgtcgatgct cggacgattc tgggcgtcgc
     3121 cagccacccg cacgacctcg gcctgaccga tagcctgcgg ctcgcgcgtg gtgatgatca
     3181 gcgtcccggc cttggccaga cgcgcgaacg acggcgtacg cagacggctg tcgagcacga
     3241 cccgcagcgg gtcggcgccg gtctcgccgt ctggcaggcg caccgtcagg cggggatcgt
     3301 cggcgagaac cgtctcgaca ccgacgagga tggcgtcacc cgcggcgcgc agccggtgcc
     3361 cctgcagccg cgcctccggc ccggtgatcc actggctctc gcccgtggaa gtggcgatac
     3421 gaccgtcgag cgaggtcgcg atcttcagag taatccgggg gcgcatcagc tttcgtccag
     3481 gccttcgtcg ccgagaaagc gcgcgaagtc gccggtgtgg cggaaatcct tgtagaccga
     3541 ggcatagcgg acataggcca cctcatcgac ggacttcagc gccttcatga tgaagtcgcc
     3601 gaccacgctg gacgggattt cggtctctcc g
//
LOCUS       DASVTM010000278         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_81638, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000278 DASVTM010000000
VERSION     DASVTM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81638"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1084
                     /locus_tag="VF633_07615"
     CDS             239..1084
                     /locus_tag="VF633_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX8470961.1"
                     /translation="MRIRAVLAAIGALAVLAVAGATQAQTCDRSAAQWSGQAMSNAIS
                     VYTLEWAPFGQTEMGWETYVPLLQKELRTDCAPGTPDFAEALAAFQGRYALTPTGVFD
                     QPTFAVLKGVLQERRPFVMARTRDECPEPPPLNQLGYLSPEEEHADRLTRLLRRDTLD
                     AYRAMVAAARRDVPAIAADPELLQIFSGFRDPEADAARCAAQGNCDGVRRAVCSPHRT
                     GAAVDLYVGHVEGMDVDSTDPASRRFMAHGPTYRWLVANAARFGFVPYLYEPWHWEYV
                     GVSGG"
     gene            1081..1551
                     /locus_tag="VF633_07620"
     CDS             1081..1551
                     /locus_tag="VF633_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470962.1"
                     /translation="MRRRSLTTGERSLARSVFADAIRLDQVRLFASPFDRAFVAGVVL
                     GRSWIVWPGRDLPSDIALAPLSAQATLIHELVHVWQAQQGVNLLLAKLKAGDSAASYA
                     YPLDPACGWSALNIEQQASAVEHRFRLSRGQRTRADAAFYDRLCPIWNAEQGVI"
     gene            1653..2255
                     /gene="wrbA"
                     /locus_tag="VF633_07625"
     CDS             1653..2255
                     /gene="wrbA"
                     /locus_tag="VF633_07625"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX8470963.1"
                     /translation="MAKVLVLYHSTYGHLETMAKAVAEGAGSVEGVTVDIKRVAETVP
                     EALAISSHYKMDQEAPIVTVDDLKNYDAIIVGAGTRFGTMASQMQAFWDTTGGLWFSG
                     ALIGKVGGAFTSTASQHGGQESTLLGFHKTLLHHGLIVVGLPYNYQDQMTLEAVHGGS
                     PYGASTITGGDGSRMPSEIDLGGARHQGKHIAEIAKKLHG"
     gene            2260..3021
                     /gene="ygiD"
                     /locus_tag="VF633_07630"
     CDS             2260..3021
                     /gene="ygiD"
                     /locus_tag="VF633_07630"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX8470964.1"
                     /translation="MTTQPAIFFGHGSPMNALGGPYTAAWRELGEAVGKPKGVVMISA
                     HWETRGLGVTAQLRPETIHDFGNFGPELHAVQYPAPGSPALAARVASLTNAHAVEAWG
                     LDHGTWGVLVHVWPDADVPVVQLSLDRTLTAREHYELAKALRPLREEGIVIAGSGDFV
                     HNLRTWKRTGDDAPYVWATGFNEAVKAAFVRGDHDALIDWVDLAEDSQLSVPTDEHYL
                     PLLYVAAQAKPGEPVTFFNDEITGGSISMTGVRIG"
BASE COUNT          524 a         1134 c         1010 g          510 t
ORIGIN      
        1 cggcagggtg acgggcaggg ccaccccgaa cgccgccaag ggcaggcagg gcccggagaa
       61 ggccgccagc acaccattgc tgcggcgcgg ggaactggaa gcggagacgt cggaggacat
      121 gcggcgatcc gtgcggcggc cggaggattc ggccagcctc tgttatcgca gctttgccgc
      181 cggacggaga aggccaattc cgcttgcgag actggccgtc atcaggcttg ctccgcccat
      241 gagaatacgc gccgtcctcg ccgccattgg agcgctcgcc gtgctggccg tggcaggtgc
      301 aacgcaggcc cagacgtgcg accggtcggc ggcccagtgg tcggggcagg ccatgtccaa
      361 cgccatctcg gtctacactc tggagtgggc tccgttcggc cagactgaga tgggctggga
      421 gacctacgtc cccctgttgc agaaggagct gcgcaccgat tgcgcgcccg gcacgcccga
      481 cttcgccgag gcgctggcgg cgtttcaagg gcgctatgcc ctcaccccca cgggggtgtt
      541 cgaccaaccc accttcgccg tcctgaaggg cgtgttgcag gagcgccggc cattcgtcat
      601 ggcacgcacc cgtgacgaat gtcccgagcc gccgccgctg aaccagctcg gctacctgtc
      661 gccggaggag gagcacgccg accgcctgac ccgcctgctg cgccgggaca cgctcgacgc
      721 ctaccgcgcc atggtcgccg ccgcccgccg cgacgtcccg gccatcgccg ccgatccgga
      781 gctgctgcag atcttctccg gcttccgcga tcccgaggcc gacgccgccc gctgcgccgc
      841 gcaaggcaat tgcgacggcg tccgccgcgc cgtctgctca ccccaccgca ccggcgccgc
      901 ggtcgatctg tacgtcggcc acgtcgaggg tatggacgtc gactcgaccg accccgccag
      961 ccgccgcttc atggcccacg gcccgaccta ccgctggctc gtcgccaacg ccgcccgctt
     1021 tggcttcgtc ccctacctct acgagccctg gcactgggaa tacgtcgggg tatcaggcgg
     1081 ttgaggaggc ggtctctcac gacgggtgaa cgatcgctgg cccggtcagt cttcgccgac
     1141 gccatccggc tcgatcaggt ccgcctgttc gccagcccct tcgatcgcgc cttcgtcgcg
     1201 ggcgtggtcc tcggccgcag ctggatcgtc tggccgggca gggacctgcc gtccgacatc
     1261 gccctcgcgc ccctgtcggc gcaggccacc ctgatccacg agctggtcca tgtctggcag
     1321 gcgcagcagg gtgtgaacct cctgctggcc aagctgaagg ccggcgacag cgccgcctcc
     1381 tacgcctatc cgctcgaccc cgcctgcggc tggagcgcgc ttaacatcga acagcaggcc
     1441 tcggcggtgg agcaccggtt ccgcctctcg cgcggccagc gcacacgcgc cgacgccgcc
     1501 ttctacgacc ggctctgtcc gatctggaat gcggaacaag gcgtgatctg aaaccttgag
     1561 tttcggcctt cagacttgcg caagcggccc aaaggattaa atccgctacc gacgctgtta
     1621 ctgaaggcgt cccccattca aggagcgccc aaatggcgaa ggttctggtt ctctatcatt
     1681 ccacctatgg ccacctcgag acgatggcca aggccgtcgc cgaaggtgca ggctcggtcg
     1741 aaggcgtcac cgtcgacatc aagcgcgtcg cggagaccgt gcccgaagcg ctggccatct
     1801 cgtcgcacta caagatggat caggaagccc cgatcgtcac cgtcgacgac ctgaagaact
     1861 atgacgctat catcgtcggc gccggcaccc gcttcggcac catggcctcg cagatgcagg
     1921 cattctggga cacgaccggc ggcctttggt tctcgggcgc cctgatcggc aaggttggcg
     1981 gcgcgttcac ctccaccgcc agccagcacg gcggtcagga gtcgactctg ctcggcttcc
     2041 acaagaccct gctgcaccac ggcctgatcg tcgtcggcct gccatacaac tatcaggacc
     2101 agatgaccct ggaagccgtc cacggcggga gcccctacgg cgcctcgacc atcactggtg
     2161 gcgacggcag ccgcatgccg agcgagatcg acctcggcgg cgctcgccat cagggcaagc
     2221 acatcgcgga aatcgcgaag aaactgcacg gctaaggcca tgacgaccca accggcgatc
     2281 ttctttggtc acgggtctcc gatgaacgcg ctgggcggtc catacaccgc cgcctggcgc
     2341 gagctcggcg aggcggtggg caagccgaag ggcgtggtca tgatctcggc ccactgggag
     2401 acgcgcggcc tcggcgttac agcccaactg cgtcctgaga ccatccacga cttcggaaac
     2461 ttcggcccgg aactgcacgc ggtgcagtac ccggcgcccg gatcgccggc cctggccgct
     2521 cgcgtagctt cgctgactaa cgcccatgcc gtcgaggcct gggggctgga tcatgggaca
     2581 tggggcgttc tggtccacgt ctggcctgat gccgacgtgc cggtcgtcca gctctcgctc
     2641 gaccgcaccc tgaccgcccg cgaacactat gagctggcca aggccctgcg gccgttgcgc
     2701 gaggagggga tcgtgatcgc cggctccggc gacttcgtcc acaacctgcg cacctggaag
     2761 cggaccggcg acgatgcgcc gtatgtttgg gccaccggct tcaacgaggc ggtcaaggcc
     2821 gccttcgtgc gcggcgacca cgacgccctg atcgactggg tcgacctcgc cgaggactcg
     2881 cagctcagcg tcccgaccga cgaacattac ctgccgctgc tttatgtggc cgcgcaggcc
     2941 aagcccggcg aacccgtcac cttcttcaac gacgagatca ccggcggctc catctcgatg
     3001 accggcgtcc ggatcggctg atctatacag aaccgtgatt tgtaaagact cggattgcct
     3061 gtagagttcg taggtcaacc ggaggtgaga atgcaactgc gacccttagc catcatcggc
     3121 gtcgcctgtt caacgctgat ggtctcgaca ggcgcgctgg cggaccggcc cgtaaagt
//
LOCUS       DASVTM010000279         4475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_612070,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000279 DASVTM010000000
VERSION     DASVTM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612070"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..1932)
                     /locus_tag="VF633_07635"
     CDS             complement(385..1932)
                     /locus_tag="VF633_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HEX8470965.1"
                     /translation="MSQTDTTASPTKSIAPKGAGLAFAYVTTLFFAWGFATSLVDPLI
                     AAVRGVFELSFTEALLTQFAWFTAYGVFSLPAAAILSRLGYAKSIVSALGVMVIGALI
                     VPVSTLMNFYPGVLIALFVIGGGVTLLQVAANPLAASLGSPGKSHFRLVLSQAFNSLG
                     TVVGPLLGATVMLSGGIFVAGGVTELASFEGIVLLVLGLVLGAVAGGLIGALKRSFRS
                     WLILGAAIGLGVGWLVFLQHYVGQVEATGLAAAAGTMAPAAPSAATLDPALRAETLRN
                     IDSIFFVLAVLFAALGGFIWLIRSKLKMSGQAAESGHVESPLKALSSKWAVAGAAAIF
                     VYVGSEVSIGSLMTNLLHSEGTLGLRIEDAGRLVALYWAGALMGRFAGSALLTRAPAA
                     FLLTVCTVAAAVLSFVVFQSNGATAAFAALSIGLFNSIMFPTIFTLTLERSTAPASAT
                     SGLLVFGIIGGALLPLLAGSIADTSGNLNHAFIVPLIGYVLLTIFAVAAMRTRPVTAA
                     GVTASSH"
     gene            complement(2168..2866)
                     /locus_tag="VF633_07640"
     CDS             complement(2168..2866)
                     /locus_tag="VF633_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX8470966.1"
                     /translation="MKLLNTVTIAGAAALALLASAGAASAQDVAVTANLAVTSDYVFR
                     GFSQTDENPAVQGGFDITKGSFYVGAWASNVDFNDSTDAEVDLYGGYRTEVSGFAVDV
                     GLIGYGYVSSPEGSNYPYLEAKLAVSRAIGPVTAGAAVYYSPDFFGADDKATYVEANL
                     AYSPITNLTLSGAVGEQFLDVSDDYVTWNVGATYAFAGTPLALDVRYSDTDVDTPSIP
                     ISDGRVFATLKATF"
     gene            complement(3007..4362)
                     /locus_tag="VF633_07645"
     CDS             complement(3007..4362)
                     /locus_tag="VF633_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8470967.1"
                     /translation="MSHIWNRVPGVLALLILIAGLFYAGGAFAQDTATPAPALLAHQA
                     ALDLNGADTAWLATSSALVLLMTLPGLALFYGGMVRRKNVIATITQSVGVFAVVTLVW
                     FIAGYSLAFGKNPVEAYNSYIGSIQSLFLNGVTIETAHSLLPGIPELVFISYQMTFAI
                     ITPALITGAFAERFKYSALLLFTALWSLLVYSPIAHWVWGGGFLGAAGVLDFAGGAVV
                     HVNSGVAGLVCALFLGRRKGYGTETITPHNPVLTMIGASLLLVGWIGFNAGSAGAANG
                     LMGAALFNTIAAAAAAALTWKLIEVMHKRKPSLIGMLSGIVAGLVAITPAAGFVDPKG
                     AVIIGLIAGPACYVSSVWLKKLLRYDDSLDAFGIHGAGGLVGALLTGVFATTTINSLS
                     EGANIGTQALGLAWTIAYSAVGTLIILIICKFTTGLRVTDAEEAEGLDTVLHGESLEH
                     S"
BASE COUNT          862 a         1406 c         1528 g          679 t
ORIGIN      
        1 cgcttgcgcc tgaacagatc gcctttggcg ccgctgggtt gcccgatcta gtcgggcaat
       61 gacgatgtgt atgatgatgc atcaggggcg aactccgcat gatcgcgagc acaaaccacc
      121 aaacgatcag tatcttagcc ggctcctaac cggctcgatc cagcaacgcg ctgaccgcgc
      181 gtacacttgc gcccaaacgg tctttgacat cgctatcccg gacaccgccg aactgcactc
      241 ggttggactc gaggccaatc cctcccccga aacgcccaac aaaaaagtcg ggcgagatcc
      301 gcgccttgaa ggcatcagct ctcacccgac ttccaatttg gactctctgg accctgtttt
      361 ttcagagtcc agacctgccc gaccctagtg gctcgaagcc gtcactcccg ccgccgtgac
      421 cggcctggtc cgcatggcgg cgacggcgaa gatcgtcagc agcacatagc cgatcagcgg
      481 cacgatgaag gcgtggttca ggttgccgct cgtgtcagcg atcgacccgg ccagcaacgg
      541 caacagcgca ccgccgatga tgccgaacac cagcaggccg gaggtcgccg aggccggcgc
      601 cgtcgaacgc tcgagcgtca gggtgaagat ggtcgggaac atgatcgagt tgaacaggcc
      661 gatcgacagg gccgcgaagg cagccgtggc gccgttgctc tggaacacga cgaagctcag
      721 gaccgccgcc gccacggtgc agaccgtcag caggaaggcc gccggagcgc gcgtcagcag
      781 ggcgcttccc gcgaaccggc ccatcagtgc gccggcccag tagagggcga ccaggcgacc
      841 ggcgtcctcg atgcgcaggc ccagcgtgcc ctcggagtgc agcaggttgg tcatcaggct
      901 gccgatcgac acttccgaac cgacgtagac gaagatggct gcggcgccgg ccaccgccca
      961 tttggacgac agggccttga gcggcgactc gacgtggccg gactccgcag cctgaccgga
     1021 catcttcagc ttcgagcgga tcaaccagat gaagcccccc agagcggcga acagcacggc
     1081 gaggacgaag aagatggagt cgatgttgcg cagggtctcg gcgcgcaggg ccgggtccag
     1141 tgtcgcggcg gaaggagcag caggagccat cgtcccggca gcggcggcga gaccggtcgc
     1201 ctcgacctgt ccgacgtagt gctgcaggaa gaccagccag ccgacgccca gaccgatcgc
     1261 ggcgcccagg atcaaccagc tacggaacga gcgcttgagc gcaccgataa ggccccctgc
     1321 gaccgcgccg agcacgaggc cgagcaccag caggacgatg ccctcgaacg aggcgagctc
     1381 cgtaacgccg cctgcgacga agatgccgcc tgacagcatg accgtcgcgc cgagcaacgg
     1441 tccgacgacg gtgccgagcg agttgaaggc ctgagacagg acgaggcgga agtgcgactt
     1501 gcccggcgat cccagcgagg ccgccagcgg gttagccgcg acctgcagca gggtgacccc
     1561 gccgccgatc acgaacagag cgatcagcac acccggatag aagttcatca gcgtcgacac
     1621 gggcacgatc agcgccccga tcaccatcac acccagcgca ctgacgatcg acttggcgta
     1681 ccccagcctg gacaggatcg ccgccgccgg cagcgagaag acgccatagg ccgtgaacca
     1741 ggcgaactgg gtcagcaggg cctcggtgaa gctcagctcg aacacgccgc gcaccgcagc
     1801 gatcagcgga tcgaccagcg acgtcgcaaa gccccaggcg aagaacaggg tggtgacgta
     1861 ggcgaaggcg aggccggcgc ccttgggcgc gatactcttc gtggggctgg cggtcgtgtc
     1921 tgtctggctc atggggtcct ggcgcggttc cttgggcggc cgcgccggtg cgtgaccgca
     1981 ctgcgaagct cttgatcacc ttgcttccac ggaccgaggc cggtggtcca gccgattcgc
     2041 ccacccgtaa cgaaaagtgg ccccgcagac aggctgcggg gccacgttcc agtgcatacc
     2101 ccggagcggg gttgcgtcct ggccgcgtct cacatcgcgg tcaggccatc cacactacgg
     2161 tctccgccta gaacgtagct ttgagggtcg cgaagacgcg gccatcgctg atcgggatcg
     2221 agggggtgtc aacgtcggtg tcggagtagc ggacgtccag agccaggggc gtgccggcga
     2281 aggcgtaggt cgcgcccacg ttccacgtga catagtcgtc gctgacgtcg aggaactgct
     2341 cgccgaccgc accggacagg gtcaggttgg tgatcggcga ataggccagg ttggcctcga
     2401 cataggtagc cttgtcgtcg gcgccgaaga agtcgggcga gtagtagact gcggcgccgg
     2461 ccgtgaccgg accgatggcg cgcgagacgg ccagcttggc ctcgagatac gggtagttgg
     2521 atccttccgg cgaagagacg tagccgtagc cgatgaggcc tacgtcaacg gcgaagccgc
     2581 tgacttccgt gcgatagccg ccgtacagat cgacctcggc gtcggtggag tcgttgaagt
     2641 cgacgttgga cgcccaggcg ccgacgtaga agctgccctt cgtgatgtcg aagccaccct
     2701 gaacggcggg gttctcgtcg gtctggctga agccgcggaa gacgtagtcg ctggtgacgg
     2761 cgaggttggc ggtgaccgcg acgtcctgag ccgaagcggc gccggccgag gccagcaggg
     2821 cgagggcggc ggccccggcg atagtgacgg tgttgagcag tttcatggat atccccttcc
     2881 agtgtgtttg agagtcgatc ggtgggggtg agagccccgt gttccaatct gcgtttgtcg
     2941 ttggcgtacg ttcgttagct tggggtggta cgccggatgc ggtcccagcc gcatccggcg
     3001 ctgtggctac gagtgttcga gcgactcgcc gtgcaggacg gtgtcgagcc cttcggcttc
     3061 ttcggcatcc gtaacccgca gtcctgtggt gaacttgcag atgatcagga tgatcagggt
     3121 gccgacggcg ctgtaggcga tggtccaggc caggccgagc gcctgggtgc cgatgttcgc
     3181 gccttcagac aggctattga tcgtggtggt ggcgaacacg ccggtcagca gagcgccgac
     3241 cagaccaccc gcaccgtgga tgccgaaggc gtccaggctg tcgtcatagc ggagcagctt
     3301 cttgagccac accgaagaga cgtagcaggc cggtccggcg atcaggccga tgatgacggc
     3361 gcccttggga tcgacgaagc ccgcagccgg agtgatggcg acgagaccgg cgacgatgcc
     3421 ggatagcatg ccgatcagcg agggtttgcg cttgtgcatg acctcgatga gcttccaggt
     3481 cagggcggcg gcggcggcgg cggcgatcgt gttgaacagc gcagcgccca tcaggccgtt
     3541 ggctgcaccg gcggaaccgg cgttgaagcc gatccagccc acgagcagca acgatgcgcc
     3601 gatcatggtg aggacggggt tgtgaggcgt gatggtctcg gtgccgtaac ccttgcggcg
     3661 gccgaggaag agcgcgcaga cgaggcccgc gacgcccgag ttgacgtgaa cgaccgcacc
     3721 accggcgaag tccagcaccc cggccgcgcc gaggaagccg ccgccccaca cccagtgggc
     3781 gatcggcgaa tagaccagca acgaccacag ggccgtgaac agcagcagcg ccgagtattt
     3841 gaagcgctcg gcgaaggcgc cggtgatcag cgcaggcgtg atgatggcga aggtcatctg
     3901 ataggagatg aacaccagct ccgggatgcc cggcagcagg ctgtgcgccg tctcgatggt
     3961 cacgccgttc aggaacagcg actggatgct gccgatgtag gagttgtagg cctcgaccgg
     4021 gttcttgccg aacgccagcg aatagcctgc gatgaaccac accagggtca cgacggcgaa
     4081 cacgccgacc gactgggtga tcgttgcgat cacgttcttg cgccgcacca tgccgccgta
     4141 gaacagcgcc agacccggca aggtcatcag caggaccagg gcggatgatg tggccagcca
     4201 ggccgtgtcc gccccgttca gatcaagtgc ggcctgatgg gccagcagcg ccggtgcagg
     4261 cgtggccgtg tcctgagcga acgctccgcc ggcatagaac agcccggcga tcaatatgag
     4321 cagggcgagg acgcccggta cccgattcca gatatgtgac atgtgcgtgg agcccctcat
     4381 gcaatctcgc aggtgcgaga agatcacggg ctcccgacat ttcgcaagtg gctttgccgt
     4441 atgcggcgca attttcgtgc tgtcgtatta tttca
//
LOCUS       DASVTM010000280         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_507958,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000280 DASVTM010000000
VERSION     DASVTM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507958"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF633_07650"
     CDS             <1..255
                     /locus_tag="VF633_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX8470968.1"
                     /translation="RRLLEMAENAATVVGIELLAAAQGIEFHRPLTSSTALEQVFATV
                     REAAAAYGSDHYFAPDIARATDGVRAGRYRQFAGGLLPSV"
     gene            complement(289..1335)
                     /gene="fni"
                     /locus_tag="VF633_07655"
     CDS             complement(289..1335)
                     /gene="fni"
                     /locus_tag="VF633_07655"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX8470969.1"
                     /translation="MSDDSSIPGRPILGRKDQHLDVVLSGAGRHARDAGFEAVRFVHE
                     ALPDLDHAKIDLGADFLGRRLQAPLLISAMTGGPARAEAINARLAEAAQHLGIALAVG
                     SQRAALEGEGGSGGLNASLRDRAPDTPILANIGAAQLTRGFGVDEARRAMAMIGADAL
                     IVHLNPLQEACQPEGDRDWWGVGAALEALIRKLDAPVVIKETGAGLSAATAIRLVDMG
                     AAAIDIAGAGGANWALIEGERATERADKDHAAAFADWGIPTARAIAEVRAACPGAVII
                     GSGGVRDGLDAAKAIRLGADVVGQAANVLAAATVSTEAVVAHFQLVIRQLRTVCFCTG
                     SANLAGLKRAALQR"
     gene            1414..1917
                     /locus_tag="VF633_07660"
     CDS             1414..1917
                     /locus_tag="VF633_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8470970.1"
                     /translation="MLPVWFVLIVIAVFVAMEGVAWASHRWIMHGWGWAWHQSHHEPR
                     TGLFEANDLYAVVGSVAGFGLFLIGYLSGSWLIYALASGVTLYGLAYAIFHDGLVHQR
                     WPSRWVPRHGYLRRLIQAHRLHHAVMSKGGAVSFGFLIAPDPKALAAELKALRAARRD
                     AQSRERL"
     gene            complement(1914..2852)
                     /locus_tag="VF633_07665"
     CDS             complement(1914..2852)
                     /locus_tag="VF633_07665"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX8470971.1"
                     /translation="MTDAVLAASKESIVKGSKSFRSASRLFDPAVREDAWLLYAWCRR
                     CDDEIDGQDHGFGHEELSEDEQKRRLEGLYRQTRRALAGERMDDQTFAAFQRVALRHR
                     LPERWPLDLLDGFAMDVEHREYRTLDDVMAYCWHVAGVVGVMMARVMGVFDNEVLRRA
                     QDLGLAFQLTNISRDVLEDAQNGRVYLPADWLIEAGLEPTAEAVADPNNRAAVHAVTA
                     RLLAAAEPYYDSARVGLRGLPFRSSMAIAAARGVYREIGRKVIRRGPGVWTRRVSVGK
                     AMKLWLFGRSVLIAIWTRILDRGKAPPPRPEMWTRV"
     gene            complement(2849..4366)
                     /locus_tag="VF633_07670"
     CDS             complement(2849..4366)
                     /locus_tag="VF633_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEX8470972.1"
                     /translation="MRDIVAKRAVVIGAGFGGIALAIRLQRAGVHTTLLEKRDKPGGR
                     AYVWHEEGFTFDAGPTVITDPACLEALFDGTGSQLSDYVDMLPVNPFYRLCWEDGTVF
                     DYANDQAELDRQIEALNPADVEGYRRFHAYSEELYQEGYLKLGAVPFQSFWSMIKAAP
                     ELARLESWNSVHAMVARFIEEPHLRQAFSFHTLLVGGDPFKTSSVYALIHALERRGGV
                     WFARGGTGALIAGLVKFFKDLGGDFRLNAPADRIEVEGERVTGVRLEGGEVVPADMVA
                     SNADVVHTYEKLLGHAPRGPAKAKSLKSKRFSNSLFVLHFGLKTPQPQLAHHTICFGA
                     RYRELIAEIFKGPELPEDFSLYLHAPCATDPSMAPEGMSSHYVLSPVPHLGAADIDWE
                     VQGPLYREKIIDYLEERYIPNLRRDLVTTRIFTPADFKEDLNAHLGSAFSLEPILTQS
                     AWFRTHNRDDKIPNLYVVGAGTHPGAGIPGVVGSAKATAGLMLEQAGVASARTPA"
     gene            complement(4363..>4803)
                     /locus_tag="VF633_07675"
     CDS             complement(4363..>4803)
                     /locus_tag="VF633_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX8470973.1"
                     /translation="IALDGDIDAFWAEGSPVARVGLAAALFHPTTGYSLPDAVSTARM
                     IAGLSDLSSASLRSATEQRSKSLWAERAFYRLLDRMLFKAAEPGERWKVLRRFYGLSE
                     AIVRRFYAGRSTTLDKVRLLAGKPPVPVVKAMRQLRETRSGAAA"
BASE COUNT          784 a         1681 c         1579 g          759 t
ORIGIN      
        1 cgtcgcctgc tggagatggc cgagaacgcc gcgaccgtcg tcgggattga actgctcgcc
       61 gccgctcaag gcatcgagtt ccaccgcccc ctgacaagct cgaccgcgct ggaacaggtg
      121 ttcgctaccg tccgcgaagc cgccgccgcc tacggctccg accactactt cgcccccgac
      181 atcgcgcgag caacggatgg ggtcagggct ggccggtatc ggcagttcgc gggtgggttg
      241 ctgccgtcgg tgtgactcat ccttctcccc ctgcgggaga aggacgtcct accgctgcag
      301 cgctgcccgc ttgagccctg ccaggttggc cgagccagtg cagaagcaca ccgtcctcag
      361 ctggcggatc actaactgga agtgcgcgac gaccgcctcg gtcgagacgg tggcagcggc
      421 cagcacattg gcggcctggc caacgacatc ggcgcccagt cggatggcct tggccgcatc
      481 aaggccgtcg cggaccccgc ctgagcctat gatcaccgcc cccggacagg ccgctcgcac
      541 ctcggcgatg gcacgggctg taggaatacc ccagtcggcg aaggcggcgg cgtgatcctt
      601 gtcggcgcgc tcggtcgcgc gttcgccctc gatcagagcc caattggccc cgcccgctcc
      661 ggcgatgtcg atggcggcgg cgcccatgtc gaccagccgt atggccgtgg cggcggacag
      721 gcccgcgccg gtctccttga tcaccaccgg ggcgtcgagc ttgcggatca acgcctccag
      781 cgccgcaccc acgccccacc agtcgcggtc accctccggc tggcaggcct cctgcaacgg
      841 gttcagatgc acgatcaagg cgtcggcgcc gatcatggcc atggcccggc gcgcctcatc
      901 gacaccaaag ccgcgcgtaa gctgggccgc gccgatgttg gcgaggatcg gggtgtcggg
      961 cgcccggtcg cgcagcgagg cgttcagccc gccggacccg ccttcgcctt ccagcgccgc
     1021 ccgttgcgat cccacggcca gagcgatgcc cagatgttgc gccgcctcgg ccagacgggc
     1081 gttgatcgcc tccgcccgag ccggcccgcc cgtcatggcg ctgatcaaca gcggcgcctg
     1141 caaccgccgc ccaaggaagt cggcgcccag atcgatcttg gcgtgatcca gatcaggcag
     1201 ggcctcatgg acgaaccgca ccgcctcgaa gccggcgtcc cgtgcatgac gaccggcgcc
     1261 agacaggacg acgtccagat gctggtcctt ccgaccaagg ataggacggc cgggaatgct
     1321 gctgtcgtcg ctcatggcgt caccctagcg accgcgcgct gcaagtggaa cgccaaggtt
     1381 ggagacctgc gctcctccgc gtaggatcac accatgcttc ctgtatggtt cgttctgatc
     1441 gtgatcgccg tgttcgtggc gatggaaggc gtggcctggg cctcgcatcg ctggatcatg
     1501 cacggctggg gttgggcctg gcaccagagc caccacgaac cgcgtaccgg cctgttcgag
     1561 gccaacgatc tgtatgccgt cgtcgggtcg gttgccgggt tcggcctgtt cctgatcggc
     1621 tacctgagcg gatcgtggct gatctatgcg ctggccagcg gcgtgaccct ctacgggctg
     1681 gcctacgcca tcttccacga cgggctggtg catcagcgct ggccgtcccg ctgggtgccg
     1741 cgtcacggct accttcggcg gctgatacag gcgcaccgac tgcatcatgc ggtgatgtcg
     1801 aagggcggcg cggtgtcgtt cggcttcctg atcgcgccgg acccgaaggc cctcgccgcc
     1861 gagctcaagg cgcttagagc cgctcgccgg gacgcccaaa gccgcgaacg gctctagacc
     1921 cgcgtccaca tctcggggcg gggtggcgga gccttgcccc ggtcgaggat ccgcgtccag
     1981 atcgcaatca gcacgctgcg cccgaacagc cacagcttca tcgccttgcc gaccgacacc
     2041 cggcgggtcc agacgcccgg gccgcgcctg atcaccttgc gaccgatctc gcgatacacg
     2101 ccgcgcgccg ccgcgatcgc catcgacgac cggaacggca gcccacgcag acccacccga
     2161 gcgctgtcgt aatagggctc ggcagcggcc agcaggcgcg cggtcacggc atgcacagcg
     2221 gcgcggttgt tcggatcggc cacggcctcg gctgtcggct ccagccccgc ctcgatcagc
     2281 cagtcggccg gcagatagac ccggccattc tgcgcgtcct cgagaacgtc gcgcgagatg
     2341 ttggtcagtt ggaacgccag ccccagatcc tgggcgcgac gcaggacctc gttgtcgaac
     2401 acgcccatga cccgcgccat catcaccccg acgacgcccg cgacatgcca gcaataggcc
     2461 atgacgtcgt ccagcgtccg gtactcgcgg tgctcgacat ccatggcgaa cccgtcgagc
     2521 aggtcgagcg gccagcgctc cggcagccgg tgacgcaggg ccacgcgctg gaaggccgca
     2581 aacgtctggt cgtccatccg ctctcccgcc aacgctcggc gggtctggcg atagaggcct
     2641 tccagccgcc gcttctgctc gtcttccgac agctcctcat gaccgaagcc gtggtcctga
     2701 ccgtcgatct cgtcgtcgca acgacggcac caggcgtaca gcagccacgc gtcctcgcgc
     2761 accgccggat cgaacaggcg cgaggccgag cggaagctct tggacccctt gacgatcgac
     2821 tccttcgagg cggcgaggac ggcgtcggtc atgcgggcgt tcgagcgctg gcgacgccgg
     2881 cctgttccag catcaggcct gcggtggcct tcgccgagcc aaccacgccc gggatgcctg
     2941 cgcccggatg ggtgccggcc cccacgacat acaggttggg gatcttgtcg tcccggttgt
     3001 gggtccggaa ccaggcgctc tgggtcagga tcggctccag cgagaaggcg ctgccgaggt
     3061 gggcgttgag gtcttccttg aagtcggcgg gtgtgaagat gcgggtggtg accagatcgc
     3121 ggcgcaggtt cgggatgtag cgctcttcga ggtagtcgat gatcttctcg cgatagagcg
     3181 gcccctgaac ctcccagtcg atgtccgccg cgcccagatg cggcacaggc gacaggacgt
     3241 agtggctcga catcccctcg ggcgccatcg acggatcggt ggcgcagggc gcgtgcagat
     3301 acagcgagaa gtcctcgggc agctccggtc ccttgaagat ctcggcgatc agttcgcggt
     3361 agcgcgcgcc gaagcagatg gtgtggtggg ccagttgcgg ctgcggcgtc ttgagcccga
     3421 aatgtagcac gaacagcgag ttcgagaacc gcttcgactt gagcgacttg gccttggccg
     3481 gaccgcgtgg cgcatggccg agcagcttct cataggtgtg gaccacgtcg gcgttggacg
     3541 cgaccatgtc ggcgggcacg acctcgccgc cttcaagccg aaccccggtg acgcgctcgc
     3601 cctcgacctc gatgcggtcg gccggcgcgt tgagccggaa gtcgccgccc aaatccttga
     3661 agaacttgac caggcccgcg atcagcgcgc cggtgccgcc gcgcgcgaac cagaccccgc
     3721 cacgccgctc cagcgcgtgg atcagggcat agacgctcga cgtcttgaac ggatcaccgc
     3781 caaccagcag cgtatggaag ctgaacgcct gacgcaggtg cggctcctcg atgaaccggg
     3841 cgaccatggc atggacgctg ttccagctct ccagccgcgc cagctcgggc gccgccttga
     3901 tcatcgacca gaaggactgg aacggcacgg cgcccagctt cagatagccc tcttgataca
     3961 gctcctccga ataggcgtgg aagcggcggt aaccctcgac gtcggcgggg ttgagggcct
     4021 cgatctggcg gtccagctcg gcctgatcgt tggcgtagtc gaacaccgtc ccgtcttccc
     4081 agcacagccg gtagaacgga ttgaccggca gcatgtcgac atagtcggac agctggctgc
     4141 ccgtgccgtc gaacagcgct tccagacagg cgggatcggt gatgacggtc ggaccggcgt
     4201 cgaaggtgaa gccctcttca tgccagacgt aggcgcggcc gccgggcttg tcgcgcttct
     4261 ccagcagggt cgtatgcacg cctgcacgtt gcaggcggat cgccagggca atgccgccga
     4321 accctgcgcc gatcaccacg gctcgcttgg cgacgatatc tctcatgcgg cggctccaga
     4381 ccgtgtctcg cgcaactgac gcatggcttt gacgacgggc acgggcggtt ttcccgcaag
     4441 cagccgtacc ttgtccagcg tcgtgctgcg tcctgcgtag aaccgccgca caatcgcttc
     4501 ggatagacca tagaaacggc gcaggacctt ccaacgttcc cccggttcgg ccgctttgaa
     4561 caacatacgg tcaagcagcc gatagaaggc gcgctccgcc cacagggact tggatcgctg
     4621 ctcggtcgcc gaccgcagcg aggccgacga caggtcgctg aggccggcga tcatccgcgc
     4681 cgtcgaaacc gcatccggca gcgaatagcc ggtcgtcgga tggaacaagg ccgccgccaa
     4741 accgacgcgg gccacaggcg atccctcggc ccagaaggcg tcgatgtcac cgtccagcgc
     4801 gat
//
LOCUS       DASVTM010000281         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_131560,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000281 DASVTM010000000
VERSION     DASVTM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131560"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VF633_07680"
     CDS             complement(<1..645)
                     /locus_tag="VF633_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX8470974.1"
                     /translation="MPTEIWRSVLYLPASNARAIEKARTLPCDVAVLDLEDAVAPEAK
                     AEARAAAVEAVLSGGFGPRLGVRINGLDTEWGADDLAALARTGVSLIVAPKVESSEAV
                     HALNAALPASAALIAMIETPAALMRLESIAAAGGALAGLMLGVNDLAATLGTGSSSDR
                     EPLKPWLAATVAAARMHGLIVLDGVFNRIDDGDGFDAECAQGRLYGFDGKSLIHP"
     gene            complement(673..1167)
                     /locus_tag="VF633_07685"
     CDS             complement(673..1167)
                     /locus_tag="VF633_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8470975.1"
                     /translation="MSYIARSDRPADKAEAYAEVEAEILAVLDGEPNRIARMATVASM
                     LADAFDAYFWTGFYVVDADKPRELVVGPYQGTLGCLRIAFGRGVCGTAAETGQTQLVP
                     DVHAFPGHIACDSRSESEVVVPVFDASGALIAVFDVDATEKAAFDEVDASALERLLKA
                     VFAA"
     gene            1463..>1780
                     /locus_tag="VF633_07690"
     CDS             1463..>1780
                     /locus_tag="VF633_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEX8470976.1"
                     /translation="MSDGDDTHRDEPRARRMRGPAAVAAVVVLAVVAGSVASCGDRGV
                     AFWRQGGNAAANACPRPASFTGSEFNAQEAGLRALRRAAFRGDFFAQLELGQRYAAAR
                     ATDK"
BASE COUNT          311 a          578 c          583 g          308 t
ORIGIN      
        1 cggatggatc aggctcttgc cgtcgaagcc atagagtcgg ccttgcgcgc attcggcgtc
       61 gaagccgtcg ccgtcgtcta tccggttgaa cactccgtcc agcacgatca gtccatgcat
      121 gcgcgccgcg gcaaccgttg ccgccagcca gggcttcagc ggctcgcggt ctgaggatga
      181 gcctgtgccc agcgttgcag ccagatcgtt tacgcccagc atcagtcccg cgagcgcgcc
      241 gccagccgcg gcgatgcttt ccagccgcat cagtgcggcg ggtgtctcga tcatggcgat
      301 cagagcggcg ctggccggca gggcggcgtt gagggcatgg acggcttcgc tgctctcgac
      361 cttgggcgcg acgatcaggg agacgcccgt cctcgccaac gccgcgaggt catctgcacc
      421 ccattcggta tcgagcccgt tgatccgtac gcccagacgc ggcccgaacc ctccggacag
      481 cacagcctct accgcagccg ctctagcctc ggccttggct tcgggcgcca cggcgtcttc
      541 cagatccagc acggcgacgt cgcagggcag ggtgcgcgcc ttctcgatgg cccgggcgtt
      601 ggacgccggc agataaagga cgcttctcca gatttcggtc ggcatgggat cggtccttcg
      661 cagatgacga acctaggcgg cgaagacggc cttgagcaaa cgctccagcg cgctggcgtc
      721 gacttcgtcg aatgcagcct tctcggtcgc atctacatcg aacacggcga tcaaggcgcc
      781 cgaggcgtcg aacaccggaa ccacgacctc gctctcggag cggctgtcgc aggcgatatg
      841 acccgggaag gcgtggacgt cggggaccag ctgggtctgg ccggtctcgg ccgccgttcc
      901 gcagacgccg cgaccaaacg caatccttag acagccgagc gtcccttgat agggaccgac
      961 cacgagttcg cgcggtttgt ctgcatcgac gacatagaac ccggtccaga aataggcgtc
     1021 gaaggcgtcg gccagcatcg aggcgaccgt ggccatccgg gcgatccggt tcggctcgcc
     1081 gtcgagaacc gcaaggattt cggcctcgac ctcggcatag gcttcggcct tgtctgcggg
     1141 gcgatccgag cgggcgatgt aggacattgt gaactctgga agggtcggtg cggcggtagc
     1201 agctcatata ggcgcgttgc ccggcgcgcg cgagcgtcag agtgctgtgc gcacaataac
     1261 gcactcgcct gtcgggcgac taagccaagc atctgtaatg taagaagcaa tcggagaaag
     1321 ctcgatattg tcaagtttat ggcaacgctt tctcgcataa cagtgttaac catacagcgt
     1381 tgtcactgga cccgaacgcc ggtttgcgga atggttaacg ccgctcgttc ggaaaacgcc
     1441 gcgcaaaggg attcggaacg agatgagcga cggagacgac actcaccggg atgaacctcg
     1501 cgcgcgccgc atgcgtggac cggcggcggt tgccgctgtc gtcgtgctgg ccgtcgtcgc
     1561 gggatctgtc gcgagttgtg gagatcgggg cgtcgcattc tggcgtcagg gcgggaacgc
     1621 tgcggccaac gcctgtcccc gtcccgcctc cttcaccggt tccgagttca atgctcagga
     1681 agcaggactg cgggcccttc gccgcgcagc cttccggggc gacttcttcg cccagctcga
     1741 gctgggccag cgctacgccg ccgcgcgagc caccgacaag
//
LOCUS       DASVTM010000282         3376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_773387,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000282 DASVTM010000000
VERSION     DASVTM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773387"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VF633_07695"
     CDS             <1..630
                     /locus_tag="VF633_07695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8470977.1"
                     /translation="VHRLWKDAAAAKLNPRPGETILDVAGGTGDMARRYSKMARRAQE
                     RRGGEDASVIVLDYNAEMILAGVEKGGEPEMQWTVGDAMSLPLPDASVDAYSISFGIR
                     NVADIAVALSEARRVLKPGGRFMCLEFSRPTTEVIRKAYDAWSFNAIPAIGQWVAHDR
                     DSYQYLVESIRRFPDQSAFKAMIETAGFRNVSATNLSGGVAALHFGWAI"
     gene            732..2237
                     /gene="ubiB"
                     /locus_tag="VF633_07700"
     CDS             732..2237
                     /gene="ubiB"
                     /locus_tag="VF633_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEX8470978.1"
                     /translation="MSWGSVLARHDALIPREITPLLPAWARPIAGFLHLFAGREGREG
                     RPGQRLGKAFEHLGPVAIKLGQVLATRADIFGGEFATDLGRLKDKLAPFPTDQARREI
                     ERSLGRSVESLYIDFGDPIGAASLAQAHPAWLADGRKVAVKVLRPGVERRVIRGLDAM
                     RLAARMVHRLVPVARRLEPDAFVETIARSMELELDMRLEAAAASEMKDIMEKATAEGG
                     GHMTAPGVVWDGVGKRVLTLEWAQGVPMTDPLAAEQPGLDKNALADNVVRAFLVQALD
                     YGTFHADLHEGNLFASAPAELVAVDFGIVGRLGPGERRYLAEILWGFLNRDYPRIARV
                     HFEAGYVPPHHSVEAFAQALRAVGEPVIGRAASQVSMGRLLGQLFEITALFDMHLRPE
                     LILLQKTMVSVEGVARRLNPDHDLWAAAQPVVERWILRELGPRAQVRDGVRELISGAR
                     ALSRLVQEPPRPETVVVQARGTPAWLVVCVSVATSASVAALVLSIWALISR"
     gene            2264..2812
                     /locus_tag="VF633_07705"
     CDS             2264..2812
                     /locus_tag="VF633_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470979.1"
                     /translation="MTKIALKIGLAVAAALILPMAGAAFAQSPATPTAQPAARPATGA
                     AGTLPNTFDRPAQVAPTPAPATPPAVTPSDPAKVAAAETALKKTIAALQSGTPNYADM
                     TPDLAGKVRERAETLTSLVKGFGSLNGVSHVATENGAELFLVMFANAPTQWIVGQDDE
                     GKVTVLLFRPAPAAPPVTTPAT"
     gene            complement(2831..3235)
                     /gene="arfB"
                     /locus_tag="VF633_07710"
     CDS             complement(2831..3235)
                     /gene="arfB"
                     /locus_tag="VF633_07710"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX8470980.1"
                     /translation="MTTIPEDELEFHFYRAGGPGGQNVNKVSTAVQMRFDAKNSPSLR
                     DPVRERLMKLAGSRLTLDGVILITAVRHRTQERNRADAIERLQELVDKASVAPVYRVP
                     TRPTRASKERRLVAKSHRSGIKSGRGRPVGND"
     gene            complement(3232..>3376)
                     /locus_tag="VF633_07715"
     CDS             complement(3232..>3376)
                     /locus_tag="VF633_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470981.1"
                     /translation="LRSMGVATSGPPPFGPKDRSSFLQALDQAVVRAKRALALQAASG
                     ASR"
BASE COUNT          498 a         1219 c         1096 g          563 t
ORIGIN      
        1 gtccaccgtc tgtggaagga cgccgccgcc gctaaactaa acccccgccc gggcgagacc
       61 atcctcgatg tcgcgggcgg cacgggcgac atggcccgcc gctattcgaa aatggcgcgc
      121 cgcgcacagg agcgtcgcgg cggcgaagac gcctcggtca tcgtactcga ctacaatgcc
      181 gaaatgatcc tggccggggt cgaaaagggc ggcgagcccg aaatgcagtg gaccgtaggc
      241 gacgccatgt ccctgcccct gcccgacgcc tcggtggacg cctattcgat cagcttcggc
      301 atccgcaacg tcgccgacat cgccgtggcc ctgtccgagg cgcgccgcgt gcttaagccc
      361 ggcggccggt tcatgtgcct cgagttctcg cgccctacga ccgaggtcat ccgcaaagcc
      421 tatgacgcct ggtccttcaa cgccattccc gccatcggcc agtgggtcgc ccacgatcgc
      481 gacagctacc agtatctggt cgagagcatc cgccgcttcc cggaccaatc ggcgttcaag
      541 gcgatgatcg agactgcagg gttccgcaac gtgtcggcga ccaacctgtc cggtggggtc
      601 gcagcgctgc attttgggtg ggcgatctga gcacgcagcc tccgccgcct aaccccgcgt
      661 actatcgggt cgcacccgcc gcgccgaaaa cggtgaaggt gcgcgacccg ctgagcgccg
      721 cctggcgact gatgagttgg ggctcggtgt tggcgcgcca tgacgcgctt atcccacgcg
      781 agatcacgcc cctgctgccc gcgtgggctc ggccgatcgc gggcttcctc catctgttcg
      841 ccgggcgcga aggccgcgag ggtcgacccg gccagcggct cggcaaggct ttcgagcatc
      901 tgggtcccgt cgccatcaag ctgggtcagg tcctcgccac ccgcgccgac atcttcggcg
      961 gggagttcgc caccgatctc ggccgcctga aggacaagtt ggcccccttc cctaccgatc
     1021 aggcgcgacg cgagatcgaa cgctcgctcg ggcggtcggt tgagagcctc tacatcgatt
     1081 tcggcgaccc gatcggagcc gcctcgctgg ctcaggccca tccggcctgg ctggccgatg
     1141 gtcgcaaggt cgcggtcaag gtcctgcgcc ccggcgtcga acgacgtgtc atccgtggct
     1201 tggacgccat gcgactggcg gcccggatgg tgcatcgcct cgtgccggtc gcccgccggc
     1261 tggaacccga cgccttcgtc gagaccatcg cccgctcgat ggagctggag ctggacatgc
     1321 gtctcgaggc cgctgccgcc tccgagatga aggacatcat ggagaaggcc actgccgaag
     1381 gcggtggcca catgactgcg cccggcgtcg tctgggacgg tgtcggcaag cgcgttctga
     1441 cgctggagtg ggcgcagggc gtccccatga ccgatccgct cgccgccgag cagccggggc
     1501 tcgacaagaa cgccctcgcc gacaacgtgg tgcgcgcctt cctcgttcag gcgctcgact
     1561 acggcacgtt ccacgccgac ctgcacgagg gcaacctgtt cgcttcggct ccggcggagc
     1621 tggtcgccgt cgacttcggc atcgtcggtc gcctcggccc cggcgagcgc cgctacctgg
     1681 cggaaatcct gtggggcttc ctgaaccgcg actatccgcg catcgcccgc gtccatttcg
     1741 aggccggcta tgtccccccg caccactcgg tcgaggcgtt tgcgcaggcg ctgcgggccg
     1801 tcggcgagcc ggtcatcggt cgcgccgcct cccaggtgtc gatgggccgg cttctcggac
     1861 agctgttcga aatcaccgct cttttcgaca tgcatctgcg gcccgagctg atcctgctgc
     1921 agaagacgat ggtctcggtc gagggggttg cgcgccgcct caaccccgac cacgacctgt
     1981 gggccgccgc ccagccggtc gtcgagcgct ggatcctgcg cgagctcgga cctcgcgccc
     2041 aagtccgtga cggggtccgc gagttgatct ctggagcccg cgccctgtcg aggctggtgc
     2101 aggaaccgcc ccgccctgag accgtcgtgg tgcaggcccg gggaaccccg gcatggctgg
     2161 tggtttgtgt cagcgtcgcc acaagcgcgt ctgtggccgc ccttgttctg tctatttggg
     2221 cgctcatcag tcgctgacga caggtttcgc cggagttttc cctatgacca agattgcttt
     2281 gaagatcggc ctcgcggtcg ccgccgctct gatactgccc atggccggcg ctgctttcgc
     2341 ccagtcgccg gccacgccaa ctgcacagcc agcagctagg ccggccaccg gcgccgccgg
     2401 gaccctgccc aacaccttcg accgcccggc gcaagttgca ccaacgcccg cgccagctac
     2461 tccgccggcg gttaccccgt ccgatcccgc gaaggtcgct gctgcggaaa cggcgctcaa
     2521 gaagaccatc gctgccttgc agtccggcac gccgaactat gcggacatga ccccggatct
     2581 cgcaggcaag gtccgggaac gtgctgagac cctgacctct ctcgtgaaag gctttggctc
     2641 actgaatggt gtgtcgcacg tcgctacgga gaacggcgcc gagctgttcc tcgtcatgtt
     2701 cgccaacgcc cccacccagt ggatcgtggg tcaggacgat gagggcaagg tcaccgtgct
     2761 cctgttccgc ccggcgcccg ctgcgcctcc ggtgaccaca cccgccacgt gaaccgggcc
     2821 gcagcgctgg tcagtcgttc ccgaccggcc gaccgcgccc gctcttgatg ccggaccggt
     2881 gcgacttggc caccagacgc cgctccttcg aggcccgcgt cgggcgggtc gggacgcggt
     2941 agaccggagc caccgacgcc ttgtcgacca gttcctgcag ccgctcgatg gcgtcggcgc
     3001 ggttgcgctc ctgggtgcgg tgacggacgg ctgtgatcag gatcacgccg tccagcgtca
     3061 gccgactgcc cgccagcttc atcagccgct cgcgcaccgg gtcgcgcagg gacggcgagt
     3121 ttttggcgtc gaaccgcatc tgcacggcag tcgagacctt gttgacgttc tgcccgcccg
     3181 gcccgcctgc gcgatagaag tggaactcca gctcgtcctc ggggatcgtc gtcaccggga
     3241 cgcccccgac gcagcctgca gcgccagcgc ccgcttcgcc ctgaccaccg cctgatcgag
     3301 cgcctgcaaa aagctggacc ggtccttggg tccgaatggc ggcggtcccg aggtggccac
     3361 gcccatcgag cgcaga
//
LOCUS       DASVTM010000283         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_27840, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000283 DASVTM010000000
VERSION     DASVTM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27840"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VF633_07720"
     CDS             complement(<1..263)
                     /locus_tag="VF633_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX8470982.1"
                     /translation="MKCPFCGNQDTQVKDSRPSDDGSAIRRRRSCPQCSGRFTTFERV
                     QLRELMILKRNGRRAPFDRDKLERSLNVALRKRPVQSEQIEQLV"
     gene            complement(271..1557)
                     /gene="glyA"
                     /locus_tag="VF633_07725"
     CDS             complement(271..1557)
                     /gene="glyA"
                     /locus_tag="VF633_07725"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX8470983.1"
                     /translation="MTASFVHDAYFSRGLADADPEVFSGIQGELGRQQEQIELIASEN
                     IVSKAVLEAQGSVLTNKYAEGYPGRRYYGGCEYVDITEDLARERAKELFGCAFANVQP
                     HSGAQANQAVFFALLQPGDTFLGMDLAAGGHLTHGSPANQSGKWFRPVTYKVREDNHL
                     IDYDHVAEMAEKEKPKLIVAGASAYSRHIDFKRFREIADSVGAYLFVDMAHYAGLVAG
                     GAYPDPLPHAHVVTTTTHKTLRGPRGGMILSNDMDLGKKINSAVFPGLQGGPLEHVIA
                     AKAVAFGEALKPEFKLYAKQVVANAQAMAAVLIERGLAIVSGGTDSHLMLVDLRPKGV
                     TGKATELQLEHALMTCNKNGVPFDTAPFTVTSGVRLGTPAGTTRGFGVEEFQQIGHWI
                     ADVVSSMNGGDEADPAVIAEVAGKVRDLTRRFPIYG"
     gene            complement(1632..1811)
                     /locus_tag="VF633_07730"
     CDS             complement(1632..1811)
                     /locus_tag="VF633_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470984.1"
                     /translation="MSDPLGEVIVESTRNGVYLKVTAVHVATGTEATAVGPAAEPKAV
                     ERLAITKLQRLLSRA"
     gene            complement(1811..3037)
                     /gene="hemA"
                     /locus_tag="VF633_07735"
     CDS             complement(1811..3037)
                     /gene="hemA"
                     /locus_tag="VF633_07735"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX8470985.1"
                     /translation="MYDYKAAFKTAVDQVKGEGRYRVFADLKRVRGQFPKAVRRRDDG
                     SEQDVVIWCSNDYLGMGQHPVVLEAMQAEIDAVGAGAGGTRNISGTTRSAVDLEAELA
                     SWHQKEAALLMTSGYVANEATLTTLQRILPGLVVFSDSLNHASMIAGIRNGGCERHVF
                     MHNDLAHLEQLLAAAPVNAPKLIAFESVYSMDGDIADLAGTIALAKKYGAMTYLDEVH
                     AVGLYGPTGAGVAERDGLLDGIDIVECTLGKAIGVMGGYIAADAMIIDAVRSWASGFI
                     FTTSLPPALTAGALASVRHLKAHPELRVAHQERAQTLKDRFAAAGIPVMPSESHIVPV
                     FVGDPVHTKMISDMLLEEFGVYVQPINYPTVPKGTERLRFTPSPNHDDAMMDALVSAM
                     DKLWTHCHITRMPAAA"
     gene            complement(3182..3985)
                     /locus_tag="VF633_07740"
     CDS             complement(3182..3985)
                     /locus_tag="VF633_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470986.1"
                     /translation="MSKSLLVAASIAILGATALSGCLIVSSSSDEKTIVAMPGEHLTQ
                     VEENAYAAAIADPLRPAGEVERDPLRKPAEMLAFAQIDAGDRVADIRPEEGYFTRLFA
                     RSVGPAGRVYAFVPNQTAGKENAFGDTLAAAYPNVNRITGNLDIMTFPEPLDVVFMGQ
                     EYHDFTIPRFGVDVAKMNAAVFAALKPGGLYVILDHQSAAGAGISVSGTLHRIEGAEL
                     RREVEAAGFVFDAETTAVANPDDDHSLSVFDPAIKGKTDQFVYRFRKPG"
BASE COUNT          766 a         1288 c         1368 g          771 t
ORIGIN      
        1 accagctgct cgatctgctc ggactgaacc ggacgtttgc gcagggcgac gttcagcgac
       61 cgctccagct tgtcacggtc gaagggcgcc cgacgaccgt tacgcttcag gatcatcagt
      121 tcgcgcagtt gtacccgttc aaaagtcgtg aaacgcccac tgcattgcgg gcacgaccgc
      181 cgacgacgga tggccgaacc gtcgtccgac ggccggctgt ccttcacctg cgtatcctga
      241 ttgccgcaaa acgggcattt catccgtcgg ttatccgtaa atcgggaagc ggcgcgtgag
      301 gtcgcgcacc ttgccggcga cctcggcgat caccgcagga tcagcctcgt cgccgccatt
      361 catcgatgag acgacgtcgg cgatccagtg accgatctgc tggaattcct cgacgccgaa
      421 gccgcgcgtg gtgcccgcag gcgtacccag acggacgccc gaggtgaccg tgaacggcgc
      481 ggtatcgaat ggtacgccgt tcttgttgca ggtcatcaga gcatgctcga gctgaagctc
      541 ggtcgccttg ccggtcacgc ccttgggacg cagatcgacg agcatgaggt ggctgtcggt
      601 gccgcccgaa acgatcgcca gcccgcgctc gatcagaacg gcggccatgg cctgagcatt
      661 ggcgaccacc tgtttcgcat agagcttgaa ctcaggcttc agcgcctcac cgaaggccac
      721 agccttggcg gcgatcacat gctccagcgg cccgccttgc agacccggga agacggccga
      781 gttgatcttc ttgccgagat ccatgtcgtt ggacaggatc atgccgccgc gcggaccgcg
      841 cagagtcttg tgcgtcgtgg tcgtcacgac gtgggcgtgg ggcaacggat cgggataggc
      901 cccgcccgcg accagaccgg catagtgggc catgtccacg aacaggtatg cgccgacgct
      961 gtcggcgatc tcgcggaagc gcttgaaatc gatgtggcgg ctgtaggccg aggcgccggc
     1021 cacgatcagc ttcggctttt ccttctccgc catttcggcg acgtggtcgt agtcgatcag
     1081 atggttgtct tcacgcacct tgtaggtcac cggccggaac cacttgccgg actggttggc
     1141 gggcgaaccg tgggtcaggt gaccgccagc ggccagatcc atgccgagga aggtgtcgcc
     1201 gggctgcagc aaggcgaaga acaccgcctg gttggcctgt gcgcccgaat gcggctggac
     1261 attcgcaaag gcgcaaccga acaattcctt ggcgcgttca cgggccagat cttcggtgat
     1321 gtcgacgtat tcgcagccgc cataatagcg acgaccgggg taaccctcgg catatttgtt
     1381 cgtaagaaca gagccttgag cctccagcac ggccttggag acaatgttct cggacgcgat
     1441 aagctcgatt tgctcctgct gacgacccag ttcaccctga atgccgctga agacctcggg
     1501 atcggcgtca gcgagaccgc gcgagaaata ggcgtcgtgg acgaaggaag cggtcacggg
     1561 cggcggtcct ggggctgcgt cggagggtgc gcctatctag gctcagccac gcctgaccac
     1621 aacatgtagt gtcaggcccg tgacaataac cgctgaagct tggtgatcgc cagccgctcg
     1681 accgcctttg gttcggcagc tggcccgacc gccgtcgcct cggtcccggt cgccacgtga
     1741 acggcggtga ccttcaggta gacgccgttg cgcgtggatt cgacgatgac ctcacccaga
     1801 gggtcactca tcacgccgcc gcaggcatcc gggtgatgtg gcagtgggtc cagagcttgt
     1861 ccatggccga gaccaaggcg tccatcattg cgtcgtcgtg gttcggcgag ggtgtgaagc
     1921 gcaggcgctc ggtgcccttg ggcacggtcg gatagttgat cggctggaca tacacgccga
     1981 actcttcgag cagcatgtcc gagatcatct tggtatggac cgggtcgccc acgaaaaccg
     2041 ggacgatgtg gctttcactc ggcatcaccg gaatcccggc ggcggcgaag cgatccttga
     2101 gggtctgggc gcgctcctga tgagcgacgc gcagctccgg atgcgccttg aggtggcgca
     2161 ccgaggcgag cgctccggcg gttaaagcag gcggaaggct ggtggtgaag atgaagccgg
     2221 aagcccacga tcgcaccgca tcaatgatca tggcgtcggc ggcgatatag ccgcccatga
     2281 cgccgatggc cttgccgagt gtgcattcga cgatgtcgat accgtccagc agaccgtcac
     2341 gttcggccac gcccgcaccc gtcggaccgt acaggccaac tgcatggacc tcgtccagat
     2401 aggtcatcgc gccgtacttc ttcgccagcg cgatggtccc ggccagatcg gcgatgtcgc
     2461 cgtccatcga atagacgctc tcgaaggcga tcagcttggg cgcattcacg ggcgctgcgg
     2521 ccagcagttg ttccagatgc gccagatcat tgtgcatgaa gacgtgacgc tcgcagccgc
     2581 cgttgcggat gccggcgatc atcgaggcgt ggttcaggct gtcggaaaaa accaccagac
     2641 cgggcaggat tcgctgcagc gtcgtcaggg tcgcctcgtt ggccacgtag cccgaagtca
     2701 tcagcagggc agcttccttc tggtgccagc tggccagctc ggcttccaga tcgaccgccg
     2761 aacgtgtcgt gccggagata ttacgcgtgc cgcccgcgcc tgcgcccacg gcgtcgatct
     2821 cggcctgcat ggcctcgagc acgaccggat gctgacccat gccgaggtag tcgttggagc
     2881 accagatgac gacgtcctgc tcggatccgt catcacgacg ccgcacggcc ttggggaatt
     2941 gaccgcgcac gcgcttgaga tcggcgaaga cgcgatagcg cccttcaccc ttgacctgat
     3001 cgacggcggt tttgaaggcg gccttgtagt cgtacaagat ggcggacttt cacgcggaaa
     3061 cgtggcggct atttgcgccg attgccgctt tctgtccacg aaatcggatg gacaaaacgc
     3121 cctaaccccc taattgataa cggttctcaa tagcgaagct ggcgatctgt tacgcttcag
     3181 ctcagcccgg cttgcggaag cgatagacga actggtcggt cttgcctttg atggcagggt
     3241 cgaacaccga cagcgaatga tcgtcatcgg ggttggcgac ggcggtggtt tcagcgtcga
     3301 agacgaagcc tgcggcctcg acctcgcgac gcagctcggc gccttcgatg cggtgcagag
     3361 tgcctgagac gctgatgccc gcgcccgccg ccgactggtg gtcgagaatg acgtaaagac
     3421 cgcctggctt gagcgccgcg aacacggccg cattcatctt ggccacgtcg accccgaagc
     3481 gggggatggt gaagtcatgg tattcctggc ccatgaagac cacgtcgagt ggctcgggga
     3541 aggtcatgat gtcgaggttg ccggtgatcc ggttcacgtt cggataagcc gctgcgagcg
     3601 tgtcgccaaa ggcgttttcc ttgcccgccg tctggttggg cacgaaggcg tagacgcgac
     3661 cagccggccc taccgagcga gcgaacaggc gggtgaaata gccctcttcg gggcggatgt
     3721 cggcgacgcg gtcaccggcg tcgatctggg cgaaagccag catctcggcg ggcttgcgca
     3781 acggatcgcg ctcgacttcg cctgccggac gcaatgggtc cgcgattgcg gcggcgtagg
     3841 cgttctcttc tacctgggtc aggtgttcac ccggcatggc gacaatggtc ttctcgtccg
     3901 aactgctgga gacgatcagg cagcccgaaa gcgcggtcgc tccgaggatg gcgatggagg
     3961 cggcgacgag aagcgacttc gacatgatgt catcctgaac agcggtgcgc gaagctacgc
     4021 ccggaagcgg ggccggatgc aagaagattt cggtgtcgaa acatcgccgg atgacagtcc
     4081 aacggccgtg gcgacttgac gcaggcggac ggcaggcgtt tgggtcggca tatcccgact
     4141 aagccccccg agagtgttca tggtccgctt cgcccgtctt acgtttcttc ggc
//
LOCUS       DASVTM010000284         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_932035,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000284 DASVTM010000000
VERSION     DASVTM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932035"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VF633_07745"
     CDS             <1..420
                     /locus_tag="VF633_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8470987.1"
                     /translation="LDDHLCFALYSASMAVTRLYKPMLDELGITYPQYLVLNALNDTG
                     SGGFLTVGGLATRLSLEPSTITPLVKRLEAAGLVSRARSQTDEREVRVSMTEAGNGVM
                     AKTACLGEALVAKSEMAMGDLGKLRDLVREFRTKVAA"
     gene            complement(425..1096)
                     /locus_tag="VF633_07750"
     CDS             complement(425..1096)
                     /locus_tag="VF633_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8470988.1"
                     /translation="MAASFEYTPRFKDMRIKPLKAGEAEAEQDVLRLKPGEKPCDHPD
                     CRKAATAKAPKSRERLNDFYEFCQPHAAEYNKAWNFYAGMNEAQIRAAKENEAMTGGR
                     PTWDMKASKNSREAATFAAKMGTATTAGTGSWRDSYGLFGRKGDQAPQSPTEDRRVGK
                     IERGALADLDLEPGVSKEVIKAAYHDLLKRCHPDHNQGDRGAEAKLVRVIKAYKTLKK
                     AGLAV"
     gene            1142..1435
                     /locus_tag="VF633_07755"
     CDS             1142..1435
                     /locus_tag="VF633_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX8470989.1"
                     /translation="MPEGPIATIIREKLTSALSPSRLELEDESWRHAGHHHEGGMDGK
                     SGGESHFNLTIVSQAFAGQNRVARQRAINALLAEELAGPVHALSIKALTPEEA"
     gene            1522..3330
                     /locus_tag="VF633_07760"
     CDS             1522..3330
                     /locus_tag="VF633_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8470990.1"
                     /translation="MVGSEGIGKGLVGGAGEAAQALTAILQTQYLRYLIIASWAVGLL
                     GTIGWTKALVWFAVTLMAGAIRGAFEKRMADKVGSGWGLIFPAVATITTCAWAAAPLL
                     AWFSGEPFGHALAVALLISGYVLVFAQLRGAPRQAIVISSPYGIAGLIILTSLWGGPL
                     FWPFLAVLPFTVAGMFVLVTMTMLREERIRAFQEHQAHLIEELQSARDKADAANTAKS
                     SFLGVISHELRTPMNGVLGAAQLLSATRLESTQRDYLSIIRNSGDNLLSLLNDILDMT
                     KIEAGKMTFETVDVSLEDLHRRVTGPFMAQAEAKGIEFVSTFEGDAPSIVRGDPLRVC
                     QVIHNLLSNAVKFTERGVIHYTISSRRLSDQRVAFDFAIKDSGAGISPEDLARLFQPF
                     TQVDASSTRRFGGTGLGLTIARRMANIMGGDITVNSAVGEGSTFTFSVEAEVVEWSAV
                     VAAEPTAAEIDAGQSLSVLVVEDHPVNRMILEAWLGSAGHATASAENGQIAVDMAREQ
                     AFDLIIMDVNMPVMDGLSATRLIRQTGANAETPIAVLSASARSEDHVAGLDAGADAYL
                     NKPIDFAALASLMNRAGGGRPAIRATSERGETAAAA"
     gene            3427..3918
                     /locus_tag="VF633_07765"
     CDS             3427..3918
                     /locus_tag="VF633_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8470991.1"
                     /translation="MKKFVTTAIAAAIAAGSLGLASTASAQSHGNRQEHRQEQRQDDR
                     QDRREHRQDARDDRREDRRDARDDRREYRQDQRAYSRYQRAERRYNAGRWQQPRGTRY
                     QVYGYGQRLPSSYNSYVVSDYNRYGLRAPPRGYHYVRNGNDVVLAGTNGGLITAVIAG
                     LFN"
BASE COUNT          685 a         1265 c         1297 g          730 t
ORIGIN      
        1 ctcgacgacc atctgtgttt cgccctgtat tccgcgtcga tggcggtgac gcggctgtac
       61 aagccgatgc tggatgagct ggggatcacc tatccgcagt atctggtgct gaatgcgctg
      121 aacgacacgg ggagtggggg ctttctgacc gtcggcggac tggcgacgcg gctgtcgctg
      181 gagccgagca cgatcacgcc gctggtcaag cggctggagg cggcggggct ggtcagccgt
      241 gcccgcagtc agaccgacga acgcgaggtc cgggtcagta tgaccgaggc aggcaacggc
      301 gtgatggcga agaccgcgtg tctgggcgag gcgctggtgg ccaagtcgga gatggccatg
      361 ggcgatctgg gcaagctgcg cgacctggtg cgggagttcc gcacgaaggt cgcggcttga
      421 gggactagac ggccagaccg gctttcttga gcgtcttgta ggccttgatg acgcggacca
      481 gtttggcctc ggcgccgcgg tcgccctgat tgtggtcggg gtggcagcgt ttcagcaggt
      541 cgtgataggc ggccttgatg acttccttgc tgacgccagg ctcgaggtcc aggtcggcca
      601 gggcgccgcg ttcgatcttg ccgacgcgac gatcctcggt gggtgattgc ggggcctgat
      661 cgcccttgcg accgaacagg ccgtagctgt cacgccacga accggtcccg gctgtggtgg
      721 cggtgcccat cttggcggcg aaggtcgcgg cctcacggct gttcttggag gccttcatgt
      781 cccaggtggg acggccgccg gtcatggcct cgttttcctt ggccgcgcgg atctgggcct
      841 cgttcatacc ggcgtagaaa ttccacgcct tgttgtactc ggccgcatga ggctgacaga
      901 actcgtagaa atcgttcagc cgctcgcgcg acttgggcgc cttggccgtc gcggccttgc
      961 ggcagtcggg gtggtcgcac ggcttctcgc cgggcttcag acgcagaaca tcctgctcag
     1021 cctcggcctc gccggccttc agcggcttga tccgcatatc tttgaaacgg ggtgtgtatt
     1081 cgaacgacgc agccattggc ctaagtgtaa ggtggatttg gacctcatca aggacgcatc
     1141 gatgccggaa ggccctatcg cgacgattat ccgcgaaaaa ctgacatccg ccctgtctcc
     1201 gagccgtctg gagctcgaag atgagagctg gcggcatgcc ggacaccatc acgaaggcgg
     1261 gatggacggc aagtcgggcg gcgagagtca cttcaacctg acgatcgtgt cgcaggcctt
     1321 tgccggtcag aaccgcgttg cacggcagcg cgccatcaac gctctgctgg ccgaggaatt
     1381 ggccggtccg gtccatgcgc tgtcgatcaa ggcgctgacg ccggaagaag cctgacggcc
     1441 gatcaaggaa tacaacgttc acttcgtctt agacacttat acgtagcgtg ctcccgggcc
     1501 ttcagttttc cgggaatatc gatggttgga tccgaaggta tcggcaaggg actcgtgggc
     1561 ggagccggcg aggccgccca ggccctgacc gccatcctgc agacccagta tctgcgctat
     1621 ctgatcatcg ccagttgggc agtgggcctg ttggggacca tcggatggac caaggcgctg
     1681 gtctggttcg cggtgaccct gatggccggt gcgatccgcg gcgcctttga aaaacgcatg
     1741 gccgacaagg tcggatcggg ctggggtctg atctttccgg cggtcgccac catcaccacc
     1801 tgtgcctggg ccgccgcgcc gttactggca tggttttcag gcgagccttt cgggcatgct
     1861 ttggcggttg cgctgctgat ctccggctat gtcctcgtgt tcgcccagct gagaggcgca
     1921 ccgcgtcagg ccatcgtcat ctcttcgccc tacggcatcg ccggcctgat cattctgaca
     1981 agcctgtggg gcggtcccct gttctggccg ttcctggccg tgttgccatt cacagtcgcc
     2041 ggtatgttcg tgctggtgac catgaccatg ctgcgtgaag agcgtatccg cgcgttccag
     2101 gagcatcagg cgcacctgat cgaggagctc cagagcgctc gcgacaaggc cgacgccgcc
     2161 aataccgcca agtcgagctt cctcggggtc atctcgcacg aactgcgcac gcctatgaac
     2221 ggcgtgctgg gcgcagcgca actgctgagc gcgacgcgac ttgagagcac ccagcgcgac
     2281 tatctgtcga tcatccggaa ctcgggcgac aacctgctaa gcctgctcaa cgacatcctc
     2341 gacatgacca agatcgaggc cggcaagatg acgttcgaga ccgtcgacgt gtcgctagaa
     2401 gacctgcatc gccgggtcac cggaccgttc atggctcagg cagaagccaa gggcatcgag
     2461 ttcgtctcga cctttgaagg tgacgcccca tcgatcgtgc gtggcgatcc cttgcgggtc
     2521 tgtcaggtga tccacaacct gctgtccaac gccgtgaagt tcaccgagcg gggcgtcatc
     2581 cattacacca tcagctcgcg caggctctcg gatcagcggg tcgccttcga tttcgccatc
     2641 aaggattcag gcgcggggat cagccccgag gatctggcgc ggctgttcca gcccttcacc
     2701 caggtggacg cctcctcgac gcgccgtttc ggcggcaccg ggctgggcct gaccatcgcc
     2761 cggcgcatgg ccaacatcat gggcggcgac atcacggtga actcggcggt aggagagggt
     2821 tctaccttca ccttctccgt cgaggctgaa gtggtcgagt ggagcgcggt cgtcgcggcc
     2881 gagcccacgg ccgccgaaat cgatgcaggc cagtcgctga gcgtgcttgt cgtcgaggat
     2941 cacccggtca accggatgat cctggaagcc tggctgggtt cggccggcca cgccaccgcc
     3001 tcggctgaga atggccagat cgccgtggac atggcccgtg agcaggcgtt cgacctgatc
     3061 atcatggacg tcaacatgcc ggttatggac ggtctcagcg ccacacggct gatccgccag
     3121 accggcgcca acgccgagac gcccatcgcc gtgctgtcgg cctcggctcg cagcgaagat
     3181 cacgtcgcgg gactggatgc cggcgcggat gcctatctga acaaaccgat cgatttcgcc
     3241 gcgctggcgt ccctgatgaa ccgcgcaggc ggtggtcgcc cggccatacg agcgacctca
     3301 gaacggggcg aaaccgcggc ggcagcctga accttacctg agcaatggcg ttcaggttcg
     3361 gctcaagggg ccgggactag tctgagggca agtcgaacgg ttcgactgtt caaaggataa
     3421 ctcgccatga agaagttcgt cactacagcc atcgccgccg ccatcgccgc cggttcgctg
     3481 ggtctggctt cgacggccag cgcccaatcg cacggtaatc gtcaggagca ccgccaggaa
     3541 cagcgccagg atgaccgtca ggaccgccgc gagcaccgcc aggacgcccg ggacgatcgc
     3601 cgcgaagacc gccgcgacgc ccgtgatgat cgccgcgagt accgtcagga ccagcgcgcc
     3661 tattcccgct atcaacgcgc cgaacgccgc tacaacgccg gtcgctggca gcagccgcgc
     3721 ggcacccgct atcaggtcta tggttacggc caacgtctgc cgagctcgta caacagctat
     3781 gtcgtcagcg attacaaccg ttacggcctg cgcgccccgc cccgcggcta ccactacgtc
     3841 cgcaacggca atgatgtcgt gcttgccggc accaacggtg gcctgatcac cgccgtgatc
     3901 gcgggtctgt tcaactagga agcctgccgc ccaggccccc ggcctcgtaa tatcgccccg
     3961 gagggaaacc tccgggg
//
LOCUS       DASVTM010000285         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_451491,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000285 DASVTM010000000
VERSION     DASVTM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451491"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF633_07770"
     CDS             <1..337
                     /locus_tag="VF633_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8470992.1"
                     /translation="ATPVVAATSTAPVKPAAQTPPPTPVRPAGGSAAAPAARPPIVTA
                     GLPAGAGREVVQKECGSCHGIDTVTAVGRTPQQWAEIIGQMQNMGLSASDEDLATIHA
                     YLSRQLPPK"
     gene            392..2074
                     /locus_tag="VF633_07775"
     CDS             392..2074
                     /locus_tag="VF633_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8470993.1"
                     /translation="MKLRTLAFTTLLALSSTAAANAQIMERPVGGDPVRLASGQVAGS
                     ALASGVRTYFGIPFAAPPLRDLRWREPQPVQAWTGVREAVAFPPMCPQGMRGPGQNHY
                     FGAEPTSEDCLYVNVWAPPTSAAQASEGAPGAKASKAPVVVWIYGGGFSGGSASMPWA
                     NGETLASKGVVYVTLNYRLGALGFMAHPELTAESPHHASGDYGHLDQVAALKWVQDNI
                     AAFGGDADNVTVIGQSAGSMSVSALQASPLSAGLFHRAIGLSGNVVGAGAGALATNEA
                     AEAQGVALQTALGVSSLAEMRRLPADRIIGVRGVRTGPSIDGYFMPRPPKEIFAAGEA
                     NDVGLLIGFTRDEGFSPLVQASSAEDYQAIARRTYPDQADELLAAYPADANWARNARD
                     AGRDISLGMTTRDWAAHQTRPGRQPAFGYMFSRVHPYAPGVAFIDHDPAAVGAYHAGD
                     IVYWFGNLDAFNAYRTTRNFTAVDRTLSDTMSNMIVAFATRGDPSLPGVSAPRYDAKD
                     EKLIELGEHVGPIDWPGRTRFDMLGSLKAQQQPPSSAAAPAVGSAPNAGPRF"
     gene            2405..>3046
                     /locus_tag="VF633_07780"
     CDS             2405..>3046
                     /locus_tag="VF633_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8470994.1"
                     /translation="MKTSVLLASISALALTAATGAHAQAAGPASTEAQAQSAEPGDTV
                     EEIVVTGSRIARRDYASTSPIVTVNEELLRQTATVNVEASLNQLPQFVQGQNQSAIGA
                     VANGGRASLNLRGLGETRNLILLDGRRLPLSSAFGVVDVNIIPPSILEGIETISGGAS
                     AVYGSDAISGVVNFKTKRHFEGLQIDARYGNAFEGSPETSDLSITGGVTSPDGR"
BASE COUNT          548 a          971 c          954 g          573 t
ORIGIN      
        1 ggcgacgcct gtcgtcgcag cgacatcgac ggctcctgtc aaacctgcgg cccagacacc
       61 tccgccaact cccgtccgac ctgctggagg aagtgcggcc gcgcctgctg cccggccgcc
      121 gattgtcacc gccggtcttc cggccggagc cgggcgcgaa gtggtccaga aggaatgtgg
      181 ctcctgtcac gggatagaca ccgtgacggc tgtcggtcgt acgcctcagc agtgggcgga
      241 gatcatcggt cagatgcaga acatgggtct gagcgccagc gacgaggacc tcgccactat
      301 ccacgcctat ctgtcgcgtc agctaccacc aaaataacgg gtccggcagc tcgccgacgg
      361 cggcggtgca gaaccgggcg ggaaacgcac aatgaagctc cgtacgctcg cattcacgac
      421 tcttctggcg ctgtcgtcaa cagcggccgc caatgcccag attatggaac ggcccgtcgg
      481 cggggatccg gtgcgactgg cttccggtca ggtcgcgggc tcggctctcg cgtccggcgt
      541 ccgtacctat ttcggcatcc ccttcgctgc gccgcccttg cgagaccttc gttggcgtga
      601 gccccagccc gttcaggcct ggaccggtgt tcgtgaagcg gtcgcgttcc cgcccatgtg
      661 cccacaaggt atgcgaggcc cgggtcagaa ccactatttt ggcgcggagc cgaccagtga
      721 agactgcctg tatgtgaacg tctgggcgcc cccaacctcg gcagcgcagg cttcggaggg
      781 cgcacctggc gccaaggctt cgaaagcccc ggttgtcgtg tggatctatg gcggtggttt
      841 cagcgggggc tcggcctcga tgccgtgggc caacggcgag accctggcgt ccaagggcgt
      901 cgtctatgtc acgctcaatt accgcctcgg cgccctgggg ttcatggccc atccggagct
      961 aaccgccgaa agccctcacc atgcgtcggg ggactatggt catctcgatc aggtcgctgc
     1021 gttgaagtgg gttcaggaca acatcgcagc cttcggcggg gatgccgaca acgtcaccgt
     1081 gatcggtcaa tctgctggat caatgtcggt ttcggcgctt caggccagcc ctctctccgc
     1141 cggtctcttt catcgcgcca tcggtctcag cggaaacgtc gtcggcgcag gcgcgggcgc
     1201 cttggccacc aacgaagcgg cggaggcgca aggggtcgct cttcagactg cgttgggggt
     1261 ctcgagcctc gcggagatgc gccgccttcc tgcagaccgg atcatcgggg tgcgtggggt
     1321 acggaccggg cccagcatcg acggctattt catgccacgt ccgccaaagg agatctttgc
     1381 ggccggtgag gccaatgatg tgggtctgct gatcggattc acgcgcgacg aagggtttag
     1441 ccccttggtt caagcgagtt ccgcagagga ttaccaagcc atcgctcgcc gcacctatcc
     1501 agatcaggcc gatgaacttc tggcggccta ccctgcggac gcgaactggg cgagaaacgc
     1561 ccgggacgcg gggcgcgaca tcagcctggg catgacgacg cgggactggg cggcccatca
     1621 gaccaggccg gggcgtcagc cagcatttgg ctatatgttt tcccgggtcc atccctatgc
     1681 gccgggcgtg gccttcatcg accacgatcc cgcggccgta ggcgcctatc acgcaggcga
     1741 catcgtctac tggttcggca atctggacgc gttcaacgcc tatcggacga cgcggaactt
     1801 cactgcggtg gacaggacgt tgtcggatac gatgtccaat atgatcgtcg cattcgcgac
     1861 ccgtggcgac ccgagcctgc cgggcgtaag cgcccctcgc tatgacgcga aggacgagaa
     1921 actgatcgaa ctcggtgagc acgtcggccc catcgattgg ccgggccgga cccgtttcga
     1981 catgctcgga tccttgaagg cccagcagca gccgccgtcc agcgctgcgg cgccagctgt
     2041 cggttcggcg cccaatgcgg gcccccggtt ctaggtgctg cggcagttat tcggcccaag
     2101 ctggctggat gatacttcac aggctgaaga cagcagtcag ctttagctcc gccttgactg
     2161 cttgttgctt actcgaccgc ccgtctccgc gggcttccag acatccagat agtcagtagt
     2221 tctggcagct ctcacggtgc agtaattatc gcacaagttc gtacattctg atatagcgtt
     2281 gctttgtacg cgtcggcccg acggtttctg cgtgccccta ccctgggctg cttgaaacag
     2341 gacggtctca aaaccgatcg gtagcaatga cctccacgca aagtgggggc tggagggaaa
     2401 cacaatgaag acctcagtat tacttgcgtc aatttctgcg ttggcactca cggcagcgac
     2461 aggcgcccat gcccaggctg caggtcctgc ttctacagag gcccaggctc agagcgctga
     2521 acctggcgac acggtcgaag agatcgttgt caccgggtct cgtatcgccc ggcgggacta
     2581 tgcatcgacc agtccgatcg tgaccgtaaa cgaggagctg cttagacaga cagcgacagt
     2641 caacgtcgaa gcctcgttga atcaactgcc gcagtttgtt cagggacaga accagagcgc
     2701 catcggggcc gtggccaacg gcgggcgcgc ttccctgaac ctgcggggtt tgggcgaaac
     2761 gcgaaacctc atcctcctgg atggacgccg tctgcccctg tcgagcgcct tcggcgtcgt
     2821 cgacgtcaac atcatcccac ccagcatact tgaagggatc gaaaccatct ccggaggcgc
     2881 ctctgcagtt tatggttccg acgcgatctc cggggtcgtg aatttcaaga cgaagcgtca
     2941 ttttgaaggg ttgcagatcg acgcccgcta cggaaacgcc tttgaaggct cgccggagac
     3001 atccgacctg tcgatcaccg gcggggtcac aagcccggac ggtcgt
//
LOCUS       DASVTM010000286         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_983815,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000286 DASVTM010000000
VERSION     DASVTM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983815"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VF633_07785"
     CDS             complement(<1..295)
                     /locus_tag="VF633_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8470995.1"
                     /translation="MLSDTARYAMRAMMALADRYPRPASIAEIAHDISAPRKYLEAVM
                     LRLKLEGLLTSQRGRTGGYALARRAGEISVADIIRSMDGPLALTPCASRTRFEA"
     gene            complement(325..1377)
                     /gene="tdh"
                     /locus_tag="VF633_07790"
     CDS             complement(325..1377)
                     /gene="tdh"
                     /locus_tag="VF633_07790"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX8470996.1"
                     /translation="MTDTMKALAKTGPGIGLELIDAPVPVAGPEDVLIRVHRTAVCGT
                     DIHIWNWDEWSQKNVPTPMITGHEFSGEIVAIGSDVNRPLKIGQRVSAEGHVIDLNSE
                     AARAGHFHLDPHTRGIGVNRQGAFAEFVVAPAFNVIELPDDVSDDIASMLDPFGNAVH
                     TAQQFDLLGEDVLVTGAGPIGIMAAAVARHAGARTVVLTDINDYRLALGNKVAPGVRT
                     VNTSREDLRDVMKELGLKVGFDVALEMSGSPIAFKQCVDTLIMGGGMAMLGIPSRPME
                     TDWGAIILKALTIKGVYGREMFTTWRKMLGLLKAGLDLEPLITHRMGYADYREGFEAM
                     KSGQSGKVVLNWQDAA"
     gene            complement(1384..1701)
                     /locus_tag="VF633_07795"
     CDS             complement(1384..1701)
                     /locus_tag="VF633_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX8470997.1"
                     /translation="MEGGQHLVEIDGRSVLSRADFYAVVDRVDGTPDWFGHNLDALFD
                     VLVAIWPKPTQLVWTHAAVSAAAMGPDFDILSGVLRDAGNEPRSRFTFRAFDGEAAPD
                     MTE"
     gene            complement(1702..2892)
                     /locus_tag="VF633_07800"
     CDS             complement(1702..2892)
                     /locus_tag="VF633_07800"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX8470998.1"
                     /translation="MPTDFHTRIAAELADIDAQGLTKPERIIASRQGPVIEVNGRKVL
                     NFCANNYLGLAGDDRVVEAAKAATERRGAGTASVRFICGTQDIHKELEAAIAAYLGFE
                     DAILFAAAFDANGGLFEPLFGAEDAIVSDSLNHASIIDGVRLCKAKRYRFANSDMDEL
                     ETQLKTARADGARNIVIATDGAFSMDGYIARLKDIRALADKYDALIMVDDCHATGFLG
                     PQGRGSFAHHGVEIDFLSGTFGKALGGAMGGFICATKPVVQLLKQRARPYLFSNALAP
                     AVCGASLEAIRIAQGSEGDALRTQLTANAHRYRSAMTEAGFDLLPGEHPIIPVMLGDA
                     KLAQDLAARMLELGVYVIGFSFPVVPRGAARIRSQMSAAHTFEHIDQTVAAFKQAGKE
                     LGVI"
     gene            complement(2966..>3263)
                     /locus_tag="VF633_07805"
     CDS             complement(2966..>3263)
                     /locus_tag="VF633_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8470999.1"
                     /translation="TVRIERHPGSYLRGGMAATPVNLLPEAKGYFDLAWTVVPSEGAG
                     EKVCPSVTSIRFSAPGDISFTTFAQALTPCGGRMDVSPIRPTIDDAPAGSATTT"
BASE COUNT          541 a         1050 c         1113 g          559 t
ORIGIN      
        1 aggcctcgaa ccgggtccgc gaggcgcacg gcgtcagggc cagcggcccg tccatgctgc
       61 ggatgatgtc ggcgaccgaa atctctcccg cccgccgcgc cagagcatag cccccggtgc
      121 gcccgcgctg gctggtcagc agaccttcca gcttgagccg cagcatcacc gcttccaggt
      181 atttccgggg cgcggagatg tcgtgagcga tctcggcgat cgaggcgggt cgtggatagc
      241 ggtcggcgag cgccatcatg gcccgcatgg cgtagcgggc cgtgtcagac agcatcgccg
      301 aagcctccgg tcacaagcgg gccgtcacgc ggcgtcctgc cagttgagca cgaccttgcc
      361 cgactggccg gacttcattg cctcgaagcc ctcgcggtaa tcggcgtaac ccatgcggtg
      421 ggtgatcagc ggctcgaggt cgagacccgc cttcagcagg cccagcatct tgcgccaggt
      481 ggtgaacatc tctcgcccgt agacgccctt gatcgtcagc gccttgagga tgatcgcgcc
      541 ccagtcagtt tccatcggac gcgacggaat acccagcatg gccatccccc cgcccatgat
      601 cagggtgtcg acgcattgct tgaacgcgat aggcgagccg gacatctcca gcgccacgtc
      661 gaagccgacc ttgaggccca gttccttcat cacgtcgcgc aagtcctcac gcgaggtatt
      721 cacggtgcgc acgcccggag cgaccttgtt gccgagggcg aggcggtaat cgttgatgtc
      781 ggtcagaacc acggtgcggg cgccggcatg gcgtgcgacg gcggcggcca tgatgccgat
      841 cgggccggcg ccggtgacca gcacgtcctc gcccaacagg tcgaactgct gagccgtgtg
      901 caccgcattg ccgaacggat cgagcatcga ggcgatgtcg tcggacacgt cgtcgggcag
      961 ttcgatgacg ttgaaggccg gggcgacgac gaactcggca aaggcgccct gacggttcac
     1021 gccgatgccg cgcgtgtgcg gatcgagatg aaaatgccct gcccgagcgg cttcggagtt
     1081 gaggtcgatg acgtgaccct cggccgagac gcgttggccg attttgagcg gacggttcac
     1141 gtcggagccg atggcgacga tttcgcccga gaactcgtgg ccggtgatca tcggcgtcgg
     1201 cacattcttc tgggaccact cgtcccaatt ccagatgtgg atgtcggtgc cgcagacggc
     1261 ggtccggtga acgcggatca gcacgtcctc gggacccgcg acgggcaccg gcgcgtcgat
     1321 cagctcgagg ccaatgccgg ggccggtctt ggccagcgct ttcatggtgt cggtcattcg
     1381 tacttactcc gtcatgtccg gcgccgcctc gccgtcgaag gcgcggaagg tgaaccggga
     1441 tcgtggctca ttcccggcgt ctcgtagcac gcctgagagg atatcgaagt ccggccccat
     1501 cgccgcagcc gaaacggcgg catgggtcca gaccagttgt gtcggtttgg gccagatggc
     1561 gaccaggaca tcgaacaagg catcgaggtt atggccgaac cagtcgggcg tcccatctac
     1621 gcgatcgacg accgcgtaga agtcggcgcg gctaaggacc gagcgcccat cgatctcgac
     1681 gaggtgctgg ccaccttcca cctagatgac gcccagttcc ttgcccgcct gtttgaaggc
     1741 agcgacggtc tggtcgatgt gctcgaaggt atgcgccgcc gacatctggc tgcggatccg
     1801 ggcggcgccg cgaggcacca ccgggaagct gaagccgatg acgtagacgc ccagctccag
     1861 catccgcgcc gcgagatcct gagccagctt ggcgtcgccc agcatgaccg ggatgatcgg
     1921 atgttcgccg ggcaggaggt cgaagcccgc ctcggtcatg gccgagcgat agcggtgggc
     1981 attggccgtc agttgcgtgc gcagggcgtc gccctcagac ccctgagcga tgcggatggc
     2041 ttccagcgag gctccacaga ctgcgggggc caaggcgttg gagaacagat agggccgggc
     2101 gcgttgtttc agcagctgaa ccaccggctt ggtggcgcag atgaaaccgc ccatggcccc
     2161 gccgagcgcc ttgccgaacg tcccgctgag gaagtcgatc tcgacgccgt ggtgcgcgaa
     2221 cgagccgcgt ccctgcggac ccaggaaacc cgttgcgtgg cagtcgtcga ccatgatcaa
     2281 ggcgtcgtat ttgtcggcca gcgcgcggat atccttcagc cgggcgatgt acccgtccat
     2341 cgagaaggcg ccgtcggtgg cgatgacgat gttgcgcgcg ccgtcggccc gggccgtctt
     2401 cagctgcgtc tccagctcgt ccatgtcgga gttggcgaag cgatagcgct tggccttgca
     2461 cagccgcacg ccgtcgatga tcgaagcatg gttcaggctg tcggaaacga tggcgtcctc
     2521 ggctccgaac aggggctcga acaggccgcc gttggcgtcg aaggcggcgg cgaacaggat
     2581 cgcatcctcg aagccgagat aggctgcgat ggccgcttcc agttccttgt ggatgtcctg
     2641 ggtgccgcag atgaagcgca ccgaggccgt acccgcgccg cgccgctccg tcgccgcctt
     2701 ggccgcctcg acgacgcggt cgtcaccggc gagaccgaga tagttgttgg cgcagaagtt
     2761 cagcaccttg cgcccgttga cctcgatcac cgggccctgc cgtgaggcga tgatccgctc
     2821 gggcttggtc aggccttgag cgtcgatgtc ggccagttcg gcggcgatgc gggtgtggaa
     2881 atcggtcggc atgggtcctc gggactcggc ggcgaagagg cttgccggtt acgccgattt
     2941 caccgccctg accagcgtag aggcttcagg tcgtcgtggc ggagcctgct ggagcgtcgt
     3001 cgatcgtggg ccggatcgga ctgacgtcca tgcgtcctcc gcaaggcgtc agagcctggg
     3061 cgaaggtggt gaagctgatg tctccgggcg cgctgaagcg gattgacgtc acactggggc
     3121 acaccttctc gcctgcgccc tcgctcggca ccaccgtcca cgccaagtcg aaatatccct
     3181 tggcctcggg gagaagattg accggcgtgg cggccatgcc gccccgcaga tagctgcccg
     3241 gatgccgctc gatcctgacg gtc
//
LOCUS       DASVTM010000287         8313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_508655,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000287 DASVTM010000000
VERSION     DASVTM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508655"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF633_07810"
     CDS             complement(<1..293)
                     /locus_tag="VF633_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8471000.1"
                     /translation="MTDLPVPADPRLSGPLAGVRIIEFDAIGPVPLAAMILSDMGADV
                     IRLARKSGAAWSEVGGAVLNRGRPHVSVDLKSPEDRDAVLDLVQHADALLEGFR"
     gene            487..906
                     /locus_tag="VF633_07815"
     CDS             487..906
                     /locus_tag="VF633_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX8471001.1"
                     /translation="MTTRTILFDSPFLLGFDQTRALIDRAAKAAAESYPPYNVEQRGD
                     NEVRITLAVAGFTPEDLTITLEGRQLTIAGKRDDVGKGDQAFLHRGIAARGFIRSFVL
                     ADGLEVEDASLEHGLLHVDLLRPEDAKVVQRIPIRVG"
     gene            997..1257
                     /locus_tag="VF633_07820"
     CDS             997..1257
                     /locus_tag="VF633_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HEX8471002.1"
                     /translation="MTPTLKMTTEAFAGLGAPDLVYVREISAADVMGDAKIEDARGLE
                     IDPEQTLYAVHSADGERLAVMLDRETAFAAAVAHELAPVSVH"
     gene            1431..2105
                     /locus_tag="VF633_07825"
     CDS             1431..2105
                     /locus_tag="VF633_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471003.1"
                     /translation="MVAIEGIDGSGKGTATRAVCALLEEAGLSVATQSFPNYGKTAAA
                     HAVQAVLNGEVKVDSAYYLASLFSLDRAETFMTSRLPDVDVVVFDRYVLSNAAFQMVR
                     LPEDDREAFLNWLFDYEYERLGVPRPDLNILMRIDPEIATQLVAKKAARSYTTATRDA
                     FEADSTYQQRVSDAYSSIASRQVNGPWAIVEVADSDGMKTPDAIAAECLALIQQAITA
                     KASSNG"
     gene            2098..3330
                     /locus_tag="VF633_07830"
     CDS             2098..3330
                     /locus_tag="VF633_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8471004.1"
                     /translation="MADTPIGQNVDVLIVGAGAAGMMCAIEAGKRGRRVLVVDHARAP
                     GEKIRISGGGRCNFTNTGTTVANFLGENPRFAASALKRYSQHDFIKRVDRQGIAWHEK
                     TLGQLFCDDSAKQIIRMLTDDMRTAGVTVKLNTGVASVARLRGNFEAALSDGTTVKAA
                     SLVVATGGKSIPKMGATGWGYDIARQFGLRVTETRPALVPLTFETGLLEQLQALAGVA
                     VDAVVSADVIERKGAPSFTEAMLFTHRGLSGPAILQISSYWREGEAIRVDMAPGVSVF
                     EALKSAKETNGKQAVATALGHIIPRRLADSVLQREGLQTPGLTGKLAELGNAKLNALG
                     AAVNGWTVKPVGSEGYRTAEVTLGGVDTAALDQQTMASTAVPGLYFIGEVVDITGWLG
                     GYNFQWAWSSGWVAGQAC"
     gene            3643..3972
                     /locus_tag="VF633_07835"
     CDS             3643..3972
                     /locus_tag="VF633_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX8471005.1"
                     /translation="MPLLMCPNDDAPMQTMERSGVQFDMCPTCRGVWLDRGELEKLME
                     AATSDGRASAPAGQPTYAPPPQQTPQPWGGGSAYREEPRRDERYRHEDHGHYKKKKRD
                     SIFDIFD"
     gene            complement(3976..5685)
                     /locus_tag="VF633_07840"
     CDS             complement(3976..5685)
                     /locus_tag="VF633_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471006.1"
                     /translation="MAGPIHYELYIRKTAPAPWSLTMATEDRKNAIETAEELLREHHA
                     VAVRVTKETLDPDTMEFASTTIMSRGAPEGPKKRLVDETAGPNCNVPQDMYTPHAREL
                     VGRVLEDWLMRNGATAYELLHRPDLAERLEASGVEVQHAIQKVAIPESQSTGQASHEL
                     IRHYQKLTEKAIERVIGAGRRKLFASLEDHSLADIAHRIAGSPDRAFIVGGVICGALI
                     GIRGARTRLGVLMDLADRAPAAGGPRALVLVALEQLLCELFAARTSVAEILGPSLDQG
                     GSMAAIVRMVAPREIDALVRADPRMALLMPAVDGPAARLGEHLEAGEFPILAASLARM
                     VLRELMGPRRLRPSDPVGEIDILRTLAMSLTATTGRLLTFEEVQTAFTERSRSLVTAD
                     FVAAYVGGCETVLCEAETLTRLCENVTGTSNKRSAARWLIACVTSLRFETEMRSSPVR
                     AIQKLHVLATLQRAVRASALAEHDEAQINMAIGAVGGVVEAESKLTTLLVRAGASVPQ
                     KLTALLRLAAGETGPTGPVADRAKAEAIRLFRVPDTRAALGAAPEALGEIKVLMKAVG
                     LAA"
     gene            5851..6555
                     /locus_tag="VF633_07845"
     CDS             5851..6555
                     /locus_tag="VF633_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8471007.1"
                     /translation="MANITLVDDDENIVASVSLALESHGHKITAYHDGATGLEALEAS
                     PPDLVILDVKMPRMDGMEVLRRLRQTSQIPVIMLTSKDEEIDEILGFNLGADDYIHKP
                     FSQRLLIERVKALLRRTGADGGEPEPSAEISGKAIRRGKLVMDPARHESTWDGKPVKL
                     TVTEFLLLQALAQRPGFVKSRDNLMDAAYDDQVYVDDRTIDSHVKRMRKKFRVVDGEF
                     DAIETLYGVGYRYRES"
     gene            6524..8170
                     /locus_tag="VF633_07850"
     CDS             6524..8170
                     /locus_tag="VF633_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEX8471008.1"
                     /translation="MALDIDTARADPSRDASPDSDSTSRRLKRFGGSRLGGLILALNL
                     LSLLILFGGALLLNEWRHGLVEARQETLTAQAELLANVLADKNYTVGEPIPQFNIEET
                     PKLLRDIFIPAGQRARLYDSDGFLFLDSYTVTENIPGAPLPPARPAGTPPPDKARNEQ
                     KASATFRRAEAALNAEVARALEGQPQAEVRISESGNRVVSVSIPVRHVQKILGVLTLE
                     AGDVDEILDAQRRALTPFALVALAVNLLASLLLHLFVARPVMRLSAAADEVKQGRARA
                     ISLPDLEGRKDEIGDLARSLESMTETLSDRMDAIEAFAADVSHEIKNPLTSIRSALET
                     LDLVKDQSQRDRLTALLQADVKRLDRLITDISNASRLDAELSRDRPRPVDLNILIGDI
                     VSIYETTAKPGEATVVLTPGTTETRVLGRDGPLGQVFRNLIDNARSFSPASGQVRVAL
                     QKNDIDPTRALSVIVDDDGPGIPPDNLETVFERFYTSRPKGTAFGANSGLGLSIVRQI
                     VEAHGGEVHAENRIAPDGSVAGARFQVDLPTTHLAVLRRS"
BASE COUNT         1515 a         2709 c         2663 g         1426 t
ORIGIN      
        1 cggaagcctt ccagcagcgc atcggcatgc tggaccaggt cgaggacggc gtcgcgatcc
       61 tcaggcgact tcagatcgac gctgacgtga ggccttccgc gattgagaac tgcgccgccg
      121 acttcgctcc aggccgcgcc ggacttgcgc gccagccgaa tgacgtcggc gcccatgtcg
      181 gacaggatca tcgcagcgag cggcacgggg ccgatggcgt cgaactcgat gatgcgaacg
      241 ccggcgaggg gaccggacag gcgcggatcg gcaggaacag gcagatcggt caacgaatct
      301 ccttgcgcga gtctcggcga gagttgtggc cgcgctcctg taacgcgtca atgaaccgtc
      361 ccgaccgggc ccaccttcat acgcatgctt gatacagacg aaatcggcgc catatccgca
      421 ggaccgccgg tcctcgccgc tcatgggagg cgccggttca acagtcgctt gttcgaggac
      481 tgaccgatga cgacccgcac tatcctgttc gatagcccct ttctgctggg cttcgaccag
      541 acccgcgccc tgatcgaccg cgccgccaag gccgctgccg aaagctatcc gccgtataac
      601 gtggagcagc gcggcgacaa cgaagtgcgt atcaccctgg cggtggccgg cttcaccccc
      661 gaagacctga ccatcaccct cgaaggtcgc cagctgacga tcgccggcaa acgtgacgat
      721 gtcggcaagg gcgatcaggc cttcctgcat cgtggcatcg cggcgcgcgg tttcatccgc
      781 agcttcgttc tggccgacgg gctcgaggtc gaggacgcct cgcttgagca cggcctgttg
      841 cacgtcgatc ttctgcgtcc cgaagacgcc aaggtagttc agcgcattcc catccgcgtg
      901 ggctgatccg acgcgctact cgcgccagcg acacttcggt agcggcgccg aaccgtccgg
      961 tgttcccggc gttacagatt tgaaggaggc ggtcacatga cgccgacact gaagatgaca
     1021 accgaagcat tcgccgggct gggcgcgcca gacctcgtct atgtgcgcga gatcagcgcc
     1081 gcagacgtga tgggcgacgc caagatcgaa gatgcgcgcg ggctggagat cgatccggaa
     1141 cagaccctct atgcggtgca cagcgccgac ggcgagcgtt tggccgtgat gctggatcgc
     1201 gaaaccgcct tcgccgctgc cgtggcgcat gagctggcgc cggtttcggt gcactagcat
     1261 aagagtggtc gctaacgccg gctcgcggag ccccgctgct tgagcgaagg ttcagaccgt
     1321 tggcggtggg gagccgggca gagaaagcga gtctctccaa cctctccaca gagtgtgcgt
     1381 ccgggtagtt gccgtcgtga tccctggcgg ctaaccgtcc ggccatgatc atggtcgcaa
     1441 ttgaaggcat cgacggctcc ggcaagggca ccgcgacgcg agccgtctgc gccttgctgg
     1501 aagaggcagg tctgtcggtc gcgacccaga gctttcccaa ctacggtaaa actgccgccg
     1561 cccacgcggt gcaggcggtt ctgaacggcg aggtgaaggt cgattcggcc tattatctcg
     1621 ccagcctgtt ctcgctggac cgggccgaga ccttcatgac gtccaggctg ccggacgtgg
     1681 atgtggtcgt gttcgatcgc tacgtcctgt cgaacgccgc cttccagatg gtgcgcctgc
     1741 ccgaagacga ccgcgaggcc ttcctgaact ggctgttcga ctatgagtat gagcggctgg
     1801 gcgtgccgcg tccggatctc aacatcctga tgcggatcga tccggagatc gccacgcaac
     1861 tggtggcgaa gaaggcggcc cgttcctaca ccacagcgac ccgcgacgcc ttcgaggcgg
     1921 acagcaccta tcagcagcga gtctcggacg cctattcgag catcgcgtcg cgtcaggtga
     1981 acggcccgtg ggccatcgtc gaggtggccg acagcgatgg gatgaagacg cctgacgcca
     2041 tcgccgccga atgcctcgcg ttgatccagc aggcgatcac cgccaaagcc tcatcgaatg
     2101 gctgacaccc ccatcggcca gaacgtcgat gtcctcatcg tcggggccgg cgcggctggg
     2161 atgatgtgcg ccatcgaggc gggcaaacgc ggtcgccgcg ttctggtcgt cgatcacgcc
     2221 cgtgcaccgg gtgagaagat ccgcatctca ggcggcggcc gttgcaattt caccaatacc
     2281 gggacgacgg tcgccaattt cctgggcgag aaccctcgct tcgcagcctc ggcgctgaag
     2341 cgctacagcc agcacgactt catcaagcgc gtggatcggc agggcatcgc ctggcatgag
     2401 aagacgctgg gtcagctgtt ctgcgacgac agcgccaaac agatcatccg catgctgacc
     2461 gacgacatgc ggacggcggg cgtgactgtg aagctgaaca ccggcgtggc ttcagtggcg
     2521 cggttgcgcg gtaacttcga ggcggcgctg tccgacggta cgacggtgaa ggcggcatcg
     2581 ctggtcgtgg cgacaggcgg caagtcgatc ccgaagatgg gtgcgaccgg ttggggctat
     2641 gatattgcgc gccagttcgg cctgcgggtc acggagacgc gtccggcgct ggtgcccctg
     2701 acgtttgaaa ccggactgct tgaacagctc caggcgctgg ctggcgtggc ggtagacgcc
     2761 gtggtgtcgg cggatgtgat cgagcggaaa ggcgcgccga gctttaccga ggctatgctg
     2821 ttcacccatc gcgggctcag cggcccggcc atcctgcaga tcagttccta ttggcgcgag
     2881 ggcgaagcca ttcgcgtgga catggcgccc ggtgtgtccg tgttcgaggc gctgaagtcg
     2941 gccaaggaaa cgaacggcaa acaggctgtc gcgacggcgc tgggccatat cattccccga
     3001 cgactggcgg actcggttct gcagcgcgaa ggtctgcaga cgccagggct aacggggaaa
     3061 ctggcggaac tcggcaacgc caaactcaac gcgctcggcg cggcggtgaa tggctggacg
     3121 gtgaagccgg tcggctccga aggctatcgc accgccgagg ttacgctagg cggggtcgat
     3181 acagcggcgc tcgatcagca gaccatggcc tccaccgccg tgccgggcct gtatttcatc
     3241 ggcgaggtcg tggacatcac cggctggctg ggaggctaca attttcagtg ggcctggtcg
     3301 tcgggatggg tcgcgggcca agcctgctga gccagctgcg aagtgtccct gcgacgcccc
     3361 tgcgacgtcg ggtccgcttg tccgtcgtcg gccgcgttcc taggttgatc tggcgggacg
     3421 ggcccctctg gcgtcgtctt ccgaacattt cccggcggct tcccccaagt tcgccgtgcg
     3481 gaacccggaa gaaggcgtga tgtcggaccg catcgggtgc ccccgtcggc tggttcgtcc
     3541 agccgtcggg cgcactcgaa ctcacggtcg agaggactac gccgaacgcg cgcctgtgct
     3601 gacgcggacc ctcccggcat tcgatgaacc gagaggaccg atatgcccct gttgatgtgc
     3661 cccaacgacg acgcccccat gcagacgatg gagcgctccg gcgtccagtt cgacatgtgc
     3721 ccgacctgtc gcggcgtctg gctggatagg ggtgaacttg aaaagctgat ggaggcagcg
     3781 accagcgacg ggcgcgcctc ggctccggcg gggcagccga cctatgctcc acctccgcag
     3841 cagacccctc aaccctgggg cggcgggtcg gcctaccggg aggaaccccg gcgtgacgaa
     3901 cgctaccgcc acgaggatca cggtcactac aagaagaaga agcgcgacag catcttcgac
     3961 atcttcgact gatagctagg ccgccagccc gacggccttc atcagcacct tgatctcgcc
     4021 gagagcttcg ggtgcggcgc cgagggcggc acgagtatca gggacacgga acaggcggat
     4081 ggcttcagcc ttggcccgat cggcgaccgg tcccgtgggg cccgtctcac ccgcagccag
     4141 ccgcaaaagc gcggtcaact tctggggcac ggaagccccg gcccgaacca gcagggtggt
     4201 cagcttcgac tcagcctcga ctacaccacc gacagcgccg atggccatgt tgatctgggc
     4261 ctcgtcatgt tcggcaaggg cgctggcccg caccgctcgc tgcagcgtcg cgaggacgtg
     4321 aagcttctgg attgcacgga cgggcgacga tcgcatctcg gtctcgaacc gcagggatgt
     4381 gacgcaggcg atcagccagc gtgccgccga tcgcttgtta gacgtgccgg taacgttctc
     4441 gcatagccgg gtcagtgttt cggcctcgca aagcacggtc tcgcaacctc cgacataggc
     4501 ggcgacgaaa tcggcggtga ccagacttcg ggatcgctcg gtgaaggcgg tctggacctc
     4561 ctcgaacgtg agcagccgtc cggtcgtcgc cgtcaatgac atcgccagcg tccgcaggat
     4621 atcaatctcg ccgaccggat cgctagggcg cagacgacgg ggccccatca gttcgcgcag
     4681 caccatcctg gccagagacg ccgcaaggat cgggaactcg cccgcctcca gatgctcgcc
     4741 cagtcgggct gcgggaccgt cgacagcggg catgagcagg gccatgcgcg ggtcagcccg
     4801 cacgagcgca tcgatttcgc gaggagcgac catgcgaaca atcgcggcca tgctgccccc
     4861 ctgatccaga gaaggaccaa ggatttcggc gacgctggtc cgggcggcaa acagctcgca
     4921 cagcaattgt tccagggcga cgaggaccaa agcgcgaggt ccccctgcgg ccggcgcgcg
     4981 gtccgccaga tccatgagaa caccaagccg cgtccgtgcg cccctgattc cgatcagagc
     5041 gccgcagatc acccccccga caatgaaggc ccggtcagga gagccggcga tccgatgcgc
     5101 gatatcagcg agggaatgat cctccagact ggcaaacagc ttgcggcgcc cggctccgat
     5161 gacccgttcg atcgcctttt cggtaagctt ttgataatga cggatcaact catgactggc
     5221 ctgcccggtc gactggcttt ccgggatcgc gaccttctgg atggcgtgct ggacctcgac
     5281 acccgacgct tccagcctct cggccagatc tggccggtgc agcaattcgt aggccgtggc
     5341 cccgttgcgc atcaaccagt cctcaagcac ccggcccacc aactcccgcg cgtgcggcgt
     5401 atacatgtcc tggggcacgt tgcagtttgg tcccgcggtt tcgtcaacca accgcttctt
     5461 cgggccttcc ggcgctcctc ggctcattat cgtggtgctt gcgaactcca tcgtatcggg
     5521 gtcgagcgtc tccttggtca cgcgcaccgc cacggcgtgg tgctcgcgca gcagctcttc
     5581 ggccgtttcg atcgcatttt tgcggtcctc ggtcgccatg gtcagtgacc agggagccgg
     5641 cgctgtcttg cggatataca actcgtaatg gatcggtcct gccatcgatg attactggca
     5701 cgagaggctt taagggtagg ttgaccccta atcgcttggt ccgagaggtc tgtagccgta
     5761 aagtcaccga aattagggtc gaacggttac gcttgacgcg cgttgatcgg gtcgaaccga
     5821 ttgaacgcct acgatttgag gaggccttgc ttggccaata tcaccctggt cgacgacgac
     5881 gagaacatcg tcgcctccgt gtcgctggcc ctcgaaagcc atggccacaa gatcacggct
     5941 taccacgacg gagccacggg gctggaggcg ctggaagcgt cgccgccgga tctcgtgatc
     6001 ctcgacgtca agatgccgcg catggacgga atggaagtcc tgcgtcgcct gcgccagacc
     6061 tcgcagatcc cggtcatcat gctgacgtcc aaggacgagg aaatcgacga aatcctcggc
     6121 ttcaacctcg gcgccgacga ctatatccac aagccgttca gccagcgtct gctgatcgaa
     6181 cgggtcaagg cgctgctgcg ccggacaggc gccgacggcg gtgagcccga gccctcagcc
     6241 gagatcagcg gcaaggccat tcggcgcggc aagctggtca tggatcccgc ccgccacgaa
     6301 agcacctggg acggcaagcc ggtcaaattg accgtcaccg aattcctgtt gctgcaagct
     6361 ttggcccagc gccccggctt cgtgaagagc cgcgacaatc tgatggacgc tgcctacgac
     6421 gatcaggtct atgtcgacga ccgcaccatc gacagccacg tcaagcggat gcgcaagaag
     6481 ttccgcgtgg tggacggcga gttcgacgcc atcgagaccc tgtatggcgt tggatatcga
     6541 taccgcgaga gctgatccct cacgggatgc cagcccggat tccgacagca cctctcgccg
     6601 gctgaagcgt ttcggcggat cgcggttggg gggactgatt ctcgccctga atttgctcag
     6661 cttgctgatc ctgtttggcg gagccttgct gctgaacgaa tggcgccatg ggcttgtaga
     6721 ggcccgccaa gaaaccttga cggctcaagc cgagttgctg gccaatgtgc tggccgacaa
     6781 gaattacacc gtcggagagc ctattcctca gttcaacatt gaggagacgc ccaagctatt
     6841 gcgggatatc ttcatccccg ccggccagag agcgcgcctg tacgacagcg acggcttcct
     6901 gttcctcgac agctatactg tcactgaaaa catcccgggc gcgccgcttc cgcccgcccg
     6961 ccccgcaggc acgcctcctc cagacaaggc ccggaacgaa cagaaggcgt ccgccacctt
     7021 ccgccgcgcc gaggccgcgc tcaatgccga ggtcgcgcgc gctcttgagg gccagcccca
     7081 ggccgaggtt cgtatttccg aaagcggaaa ccgcgtggtc tccgtgtcca ttcccgttcg
     7141 tcacgtgcag aaaatcctcg gcgtcctgac gcttgaggcc ggcgatgtcg acgaaatcct
     7201 cgatgcccaa cgccgcgccc tgacgccctt cgccctcgtg gcgctggctg tgaacctgct
     7261 ggcatccctg ctgctgcatc ttttcgtggc gcgaccggtc atgcggctgt cagcggcggc
     7321 tgacgaggtc aaacagggcc gtgcccgggc catctccctg ccggatctcg agggccgcaa
     7381 ggacgaaatc ggagatctgg ctcgttcgct ggaaagcatg acggagaccc tctcggaccg
     7441 gatggacgcc atcgaggcct tcgctgccga cgtctcgcac gagatcaaga acccgttgac
     7501 ctcgatccgg tcggcgctgg agaccctcga tctggtcaag gaccagagcc agcgcgaccg
     7561 gctgaccgcc ttgcttcagg ccgacgtcaa acgcctggac cgattgatca ccgacatatc
     7621 gaacgcctca cgtctcgacg cggaactgtc gcgcgaccgt ccccgtccag tcgatctcaa
     7681 catcttgatc ggcgacatcg tcagcatcta tgagaccacc gccaagccgg gggaagcgac
     7741 tgtcgtcctg acgcctggaa ccacagaaac gcgagtgctg ggccgtgacg gcccgctggg
     7801 tcaggtgttc cgcaacctga tcgacaacgc ccgatccttc agccccgcga gcgggcaggt
     7861 ccgggtggcg ctgcaaaaga atgacatcga cccaacccgg gcgctgagcg tcatagtcga
     7921 tgacgacggg ccgggcattc cgcccgacaa tctcgagacg gtgttcgagc gcttctacac
     7981 atcacggccg aagggcaccg ccttcggggc caactccgga cttggcctgt ctatcgtgcg
     8041 ccagatcgtc gaggcgcatg gaggcgaggt gcatgcggaa aaccgcatcg ctcccgatgg
     8101 cagcgtcgcg ggggcccggt tccaggtcga tctgccgacg acccatctgg ccgttctgcg
     8161 tcgcagctga ggccgatgaa acctcccgct ccagctacgg ctcccgaccc ggttcacgga
     8221 acggccattg cgcaccacca taaggacggc tggcgcggcg tgctgctgac cgggccttca
     8281 ggttcgggca agagcgatct ggccctgcgg ctg
//
LOCUS       DASVTM010000288         6404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_241057,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000288 DASVTM010000000
VERSION     DASVTM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241057"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VF633_07855"
     CDS             <1..722
                     /locus_tag="VF633_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8471009.1"
                     /translation="LFFALDMSFWHYGLHLTSVANATVLCNLTPVVVTLFAWIVLKQR
                     PRNLFLLALVLSMVGAAAMALGANHTGGADRSLLGDALSLSVTLWYAAYFLTIQAARK
                     TATAQRVMLWSTAVGMPLMLLAALVMREQIIPTGPSGWAACIGLGLVHVAGQGGVAWA
                     LGKLPATITAVTILVQPVVAGLVSWWAFGETLTPVQAIGGAIVLAAIVLAQWSTRKTG
                     PDDAARTKTGAGSEDPTPVKL"
     gene            complement(750..953)
                     /locus_tag="VF633_07860"
     CDS             complement(750..953)
                     /locus_tag="VF633_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8471010.1"
                     /translation="MATGTVKWFNPTKGFGFIQPDGGGADVFVHVTAVQKAGLQGLDE
                     NAKVEYELESQRGKTSAVNLKVL"
     gene            complement(1159..1968)
                     /locus_tag="VF633_07865"
     CDS             complement(1159..1968)
                     /locus_tag="VF633_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX8471011.1"
                     /translation="MKRDFADFWNLLWELGLALCAGFALMNAFVPPQDLPWKPLDLNQ
                     PVGQATSTKVSDFEVPFSAPAERVEGATEACMQTLRAAGVKVERVPDRNDGGFCIVQG
                     AVRITGGSVTPLAPGDVVMQCPLAVRYVIWDRQVLQPAAREVFGSRVKSVDDFGTYSC
                     RRIYGSTEVSDRPSEHARANALDVAGVTLEDGRKVSVLADWNGTGPAGSEGSVFLKRL
                     RDGACRVFSTVLTPDYNKEHANHLHLDGAPRSLCATGPSPRTQAATPAVVP"
     gene            complement(2043..3191)
                     /locus_tag="VF633_07870"
     CDS             complement(2043..3191)
                     /locus_tag="VF633_07870"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8471012.1"
                     /translation="MISIDRRALMSGALTVSGLAASPFTAFAQAGAAATGPDAALDAI
                     AAIAGGPPAIAAGIVTRAGLEWSGVRGVRQAGGTDAATLDDRWHMGSNTKAMTAAVFA
                     RLVDQDRARWAMPLAEAFPGAMIDPAFADVTVDDLMRHKAGLTDAAMPTPNVARADPR
                     SPVEQRAAVVDSALSRPPTGTKGAFAYANINYVLVGAAIERISGKSWEAMMGSELFQP
                     LGLTSAGFGAPESNINGGANPWGHRAGSEATKIPVDPTSPGADNPPLLGPAGRAHMTL
                     ADYATFVQAMIGGAPEGWLRPERIGVLTTPLPGDGYSMGWIARPESWAGDTMTIAHEG
                     SNTLWHAIVVAAPAKGLGFIVLSNGGFEGRSAAVPLIQRLIRERVAAT"
     gene            3302..4048
                     /gene="ppk2"
                     /locus_tag="VF633_07875"
     CDS             3302..4048
                     /gene="ppk2"
                     /locus_tag="VF633_07875"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX8471013.1"
                     /translation="MSKKDDYERDLETLQIELVETQAWAIEKGLNVLIIFEGRDSAGK
                     DGALKRITEYASPRQTRVVALPKPTERESTQWYFQRYVPHLPSGGEIVLFNRSWYNRG
                     GVEPVMGFCTPEQYAQFLKDAPRFERLLTDAGTLIIKLWLDISKAEQARRLEERIEDP
                     LKKFKVSSLDSEAQARWDAYSAARNRMLEETHSGHAPWTIIATDDKKTARINIIRHIL
                     HQLGRPGMTVDKPDKDVVFPADKSKDRLAV"
     gene            4244..4900
                     /locus_tag="VF633_07880"
     CDS             4244..4900
                     /locus_tag="VF633_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8471014.1"
                     /translation="MPEDRGALIAASSLFEFKVGHIFLEAGDEVDNVYFVTSGIISAV
                     AAMEDGRTVEVFMVGREGVTHPSAIAADTIHSYSRHSGQADGEARRIDACKLRELANT
                     RPGLRQVVADYSASLQAELEQSGGCNALHRADQRFAKWLLRCHDRIEGDTLNLTQEYL
                     GSMLGSQRTTVNEAAQTLQRAGAIHYSRGKIRIVDRAALERTACECYNARALEPVQRA
                     "
     gene            complement(4929..5789)
                     /locus_tag="VF633_07885"
     CDS             complement(4929..5789)
                     /locus_tag="VF633_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX8471015.1"
                     /translation="MRWQGGRQGGNVEDRRGMGGGAVTGGGIGVALLGLIGYFVFGID
                     PSTTQQVASSLGGAGQSQQGEVGTPTDEAGKFIDIIGTNIDDVWKARIEGYDSPTVVI
                     YEQGTSTGCGFGQSAMGPFYCPSDNKVYLDLGFWQEMETQLGGSGADFAKAYVLAHEY
                     GHHLQTLIGTSEKADAQQARARREADGNRYSVALELQADCYAGVWAKNAAGASNGQVS
                     VDGNDLEEGLKTASAIGDDTLQRGAGREVAPDSFTHGSSAQRVEWLRRGYQSGDMNSC
                     DTFAGLPGMS"
     gene            complement(5887..6270)
                     /locus_tag="VF633_07890"
     CDS             complement(5887..6270)
                     /locus_tag="VF633_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471016.1"
                     /translation="MKRYVFTPLIAALTFGAVLPAAAQVTPYPRYGAPGAGAGDFQRY
                     EMERLRQQSQESEALARQQSLQTRLTLMELQARRQPAPPPESPSPVLRSVERERAARE
                     AATAQRQSTVQGVSQIDSWLDRQPR"
BASE COUNT         1124 a         2194 c         1983 g         1103 t
ORIGIN      
        1 gcctgttctt cgcgctggac atgagcttct ggcactacgg cctgcacctg acctctgtcg
       61 cgaatgccac ggtgctgtgc aacctcacgc ccgtggtcgt gaccctgttc gcgtggatcg
      121 tgctcaagca gcgcccgcgc aatttgttcc tgctggctct ggtgctgtcc atggtcggag
      181 cggccgccat ggcgctcgga gccaaccata cgggcggcgc cgaccgctcg ctgctgggtg
      241 acgcactctc gctgtcggtg accctctggt acgccgccta cttcctgacc attcaggcgg
      301 cccgcaagac cgcgacggcc cagagggtca tgctctggtc caccgcagtc ggcatgccgc
      361 tgatgctgtt ggcggcgctg gtgatgcgcg agcagatcat ccccaccggc ccatccggct
      421 gggccgcctg tatcggcctc gggctggtgc atgtggcggg tcagggcggc gtcgcctggg
      481 cgctgggcaa gctcccggcc accatcaccg ccgtcaccat tctggttcag ccggtcgtcg
      541 ccggtctggt cagctggtgg gcgttcggcg agaccctgac accggtgcag gccatcggcg
      601 gcgcgatcgt tctggccgcc atcgtcctcg cccaatggtc cacgcgcaag accggcccgg
      661 acgatgccgc acgaacgaaa acgggcgcag gctccgaaga ccccacgccc gtcaaactct
      721 agttccgccc cgtcaggagc ggagccggat cagagaacct tcaggttcac cgccgaggtc
      781 ttgccgcgct gggattccag ctcgtattcg accttggcgt tttcatcgag cccttgcagg
      841 ccagccttct gaacggcggt gacgtgaaca aacacgtctg cgccgccgcc gtcgggttga
      901 atgaagccga agcccttcgt ggggttgaac cacttgaccg taccggtcgc catatctgcg
      961 ctctccataa aacgcgcttt ccaggcaggt cctccagagg agaaacccga cagtccatct
     1021 ggacggctcg ttcaggcgaa ggagcgagac atgggtttgg accccacgca aaatccggac
     1081 tgcgctgatg ttctctcgcg tctatccaca gcggtcaacc ggattcgaat caccccggaa
     1141 acgtaggttt cgtcggcttc acggcacaac ggcgggcgtc gctgcctgcg tgcggggcga
     1201 cggtccggta gcgcacaaac ttcgtggcgc gccatccaga tgcaaatggt tcgcgtgttc
     1261 cttgttgtaa tcgggggtca gaacggtcga gaaaacccgg catgcgccgt ccctcaaccg
     1321 cttgaggaaa accgagccct cagatccggc aggtcccgtc ccgttccagt cggcgagcac
     1381 cgaaaccttg cggccatcct cgagcgtcac gcccgcaaca tccagcgcat tggcccgcgc
     1441 atgctcgcta ggccggtcgc tgacctcggt cgatccatag atccggcggc aggaataggt
     1501 gccgaaatca tcgacagact tcacacgcga gccaaacact tctcgcgccg ccggctggag
     1561 cacctgccgg tcccagatga cgtagcgcac ggccagaggg cattgcatga ccacgtcgcc
     1621 gggggccagc ggagtcacgg agccgcccgt aatccgtacc gcaccctgga cgatgcagaa
     1681 gccgccgtcg ttgcggtcgg gcacgcgctc gaccttgacg cctgccgctc gcaaagtctg
     1741 catacacgcc tccgtcgcac cctcgacccg ctccgccggc gccgaaaaag gcacctcgaa
     1801 atcagacacc ttggtgctcg tcgcctgccc caccggctga ttcagatcga gcggcttcca
     1861 cggcagatcc tggggcggga cgaaggcgtt catgagtgcg aacccggcgc acagcgccag
     1921 ccccagctcc cacaacaaat tccagaaatc cgcgaagtcg cgcttcatgc cgcctcagcc
     1981 atgaccgtcg ggtaccttac tgcaacgctc gcaacgtgat ccggtcgcac tcgcctcgat
     2041 gtttaggttg ccgcgacccg ttcgcggatc aggcgctgga tcaacggcac ggccgcggac
     2101 cgcccttcga acccgccgtt cgacagcacg atgaatccca gccccttcgc cggagcggcc
     2161 accacgatcg cgtgccacag ggtgttcgac ccctcgtgtg cgatcgtcat cgtgtccccg
     2221 gcccagcttt ccggccgcgc gatccagccc atggaataac cgtcgcccgg cagcggcgtg
     2281 gtcaggacgc cgatccgttc gggcctcaac cagccttcgg gcgcaccacc gatcattgcc
     2341 tgcacgaacg tcgcatagtc ggccagcgtc atatgcgctc gccctgccgg tcccagcaaa
     2401 ggcgggttgt ctgcgcccgg cgaggtcgga tcgacgggga tcttcgttgc ttcggatcct
     2461 gcccggtggc cccaggggtt cgcgccgccg ttgatgttgc tctccggcgc gccgaacccg
     2521 gcgctggtca aaccgagcgg ctgaaacagc tcgctcccca tcatcgcctc ccacgacttg
     2581 ccgctgatcc gctcgatggc cgccccgacc agcacgtagt tgatattggc gtaggcgaag
     2641 gcgcctttgg ttcccgtcgg cggcctggag agcgcgctgt cgacgaccgc cgcgcgttgc
     2701 tcgaccggag accgtggatc ggcgcgcgcc acgttgggcg tcggcatggc cgcgtcggtg
     2761 agtcccgcct tgtgccgcat caggtcgtcc acggtgacgt cggcgaaagc gggatcgatc
     2821 atcgcgccgg gaaaggcttc cgccagcggc atggcccagc gtgcccggtc ctggtcgacc
     2881 agtcgggcaa agacagcggc ggtcatcgcc ttggtattcg accccatgtg ccagcggtcg
     2941 tccagcgtcg ccgcatcggt cccgcccgcc tgccgcacgc cgcgaacgcc tgaccattcc
     3001 agtcctgcgc gcgttacgat ccctgcagcg atggccggcg gccctccggc gatcgctgcg
     3061 atggcatcaa gcgccgcgtc cggacctgtg gccgctgccc ccgcttgcgc gaatgctgtg
     3121 aagggcgatg cggcaagtcc agagacagtc agtgcgccgg acatgagggc gcggcggtcg
     3181 atcgagatca tgcggtcggc tccggtggat cgaaggatgg acgatgggaa cgagggttaa
     3241 tgccgtatca tagtcggtga gtctgcttgc ggtctcaacc gccccggccc aatctaaccc
     3301 catgagcaag aaagacgact acgagcgcga cctcgaaacg ctgcagatcg aactggtcga
     3361 gacccaggcc tgggccatcg aaaaaggtct gaacgtcctg atcatctttg agggccgcga
     3421 cagcgccggc aaggacgggg ctctcaagcg gatcaccgaa tacgcttcac cgcgccagac
     3481 ccgcgtcgtc gccctgccca agcccaccga gcgcgagtcc acccagtggt atttccagcg
     3541 ctacgttccc caccttcctt cgggcgggga aatcgtcctg ttcaaccgct cctggtacaa
     3601 ccggggcggg gtcgagcccg tcatgggctt ctgcaccccg gaacagtacg cgcagttcct
     3661 gaaggatgcg ccgcgcttcg agcggctttt gaccgatgcc gggacgctga tcataaaatt
     3721 gtggctcgat atctcgaagg cggaacaagc cagacggctg gaggagcgga tcgaggatcc
     3781 gctgaagaag ttcaaggtct cctcgctgga ctcggaagcc caggcccgct gggacgccta
     3841 cagcgccgcc cgcaaccgga tgctggaaga gactcattcc ggccacgccc cgtggaccat
     3901 catcgcgacc gacgacaaga agacggcccg gatcaacatc atacgccaca tcctgcacca
     3961 gctgggtcga ccggggatga ccgtcgacaa gcccgacaag gatgtcgtgt ttccggccga
     4021 caagtcgaaa gaccggctgg cggtctaggc gtatcgccga cgaacacggc atggtggcgc
     4081 agcaatcgac cggggacttg aaaaggccta acgtgagcac cgtccaacag ggccagcgca
     4141 gtcagcacgg ctaagtcggt ttccgacgga cagcgcgcac cgctttcccc tatccggcga
     4201 tatgccacat gctgaactcc gcaacgtttt gatcggaaag cttttgcccg aggatcgggg
     4261 ggcgctgatc gccgcgtcgt ctttgtttga gttcaaggtc ggtcacatct tcctcgaggc
     4321 gggggatgag gtcgataacg tctacttcgt caccagcgga atcatttcag cggtcgcagc
     4381 gatggaggac ggccgcacgg tcgaggtctt catggtgggc cgcgagggcg tgacccatcc
     4441 ttccgctatc gcagccgaca ccattcacag ctattcacgg cactcgggtc aggcagacgg
     4501 cgaggcaagg cggatcgacg cgtgcaaact tcgcgaattg gcgaacaccc gccccggcct
     4561 acgtcaggtc gtcgccgact atagtgcgag cctccaggcc gagctcgaac agtctggtgg
     4621 ctgcaacgcc ctgcatcgcg ctgatcagcg tttcgccaaa tggttgctac gctgccacga
     4681 ccggatcgag ggcgacaccc tgaacctgac ccaagagtat cttggcagta tgctcggctc
     4741 gcagcgcacc acggtcaacg aggcggccca gaccctgcag agagccgggg ccatccacta
     4801 ctcgcgcggc aagatcagga tcgttgatcg cgccgcgctc gagcggacgg cctgcgaatg
     4861 ctacaatgca cgcgccttgg agccggtcca gagggcctag gggaccggcc agaaggccag
     4921 ccggtcgctc agctcatccc gggcaggcca gcgaaagtat cacagctgtt catgtcgccg
     4981 ctctgatacc cgcgccgcag ccattcgaca cgttgcgcgg atgatccgtg ggtgaagctg
     5041 tcaggcgcca cttcgcgacc ggctccacgc tgtagcgtgt cgtcgccgat ggcgctggca
     5101 gtcttcaacc cttcttcgag gtcgttcccg tcgaccgaca cctgaccgtt agacgccccc
     5161 gctgcgtttt tggcccacac gcctgcgtag cagtccgcct gaagctcaag cgccaccgag
     5221 taccggttgc cgtccgcctc gcgccgggcg cgcgcctgct gggcgtcggc cttttccgac
     5281 gtaccgatga gggtctggag gtgatgacca tattcatgag ccagcacata ggccttggcg
     5341 aagtcggcgc ccgagccgcc cagctgggtt tccatctcct gccagaagcc caggtccaga
     5401 tagaccttgt tgtccgacgg gcagtagaaa ggccccatgg cggactggcc gaatccgcag
     5461 ccggtcgatg tgccctgttc gtagatcacc accgtcggcg agtcgtagcc ctcgatccga
     5521 gccttccaca cgtcatcgat attggtgccg atgatgtcga tgaatttgcc cgcctcgtcg
     5581 gtgggagtcc ccacttcgcc ctgctgcgac tggcccgccc cgcccagcga actggcgacc
     5641 tgttgtgtcg tggacgggtc gatgccgaag acgaaatagc cgatcagccc cagcaaggcg
     5701 acgccgatgc cgccgcctgt gactgcgccg ccgcccatgc cacgccgatc ctcaacgttt
     5761 ccgccctgtc gtccgccttg ccaacgcatc gtttagtccc tcccaagtcg ccgcacgcta
     5821 atgcccaccg gccccttggg gaagtggcgc cacaagcgca gcagcaagcg atagcgacgg
     5881 aacgcgctac cgcggctggc gatccagcca ggagtcgatc tggctcacgc cctggacggt
     5941 ggactgccgc tgcgcggtgg ccgcctctcg cgcggctcgt tcccgctcga ccgaccgaag
     6001 gaccggtgac gggctttcag gtggcggtgc gggctggcga cgggcttgca gttccatcag
     6061 cgtcagccgc gtctgcagcg attgctgccg ggcgagcgcc tcgctctcct gagactgctg
     6121 cctcagccgt tccatctcgt agcgctggaa gtcgcctgct ccagcgcccg gcgcgccgta
     6181 acgtgggtag ggcgtgacct gcgccgccgc cggaaggact gccccaaacg tcagcgctgc
     6241 gatcaagggt gtgaagacat accgtttcat acggctgaac gtgggatcag acggcccatt
     6301 ctccaagcgt ggccgaacgt cgaaatgacg aagacgtatt cccccggact gacgacaacc
     6361 gtcaggaagc tcaccgcccc tgcgatcagg gcattgccca gacg
//
LOCUS       DASVTM010000289         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_932715,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000289 DASVTM010000000
VERSION     DASVTM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932715"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="rpsD"
                     /locus_tag="VF633_07895"
     CDS             complement(<1..518)
                     /gene="rpsD"
                     /locus_tag="VF633_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX8471017.1"
                     /translation="MSKRHSAKYKIDRAMGENLWGRAKSPVNKRSYGPGQHGQRRKSK
                     VSDFGLQLKAKQKLKGYYANLTEKQFRKTYDEAARRKGNTSETLIGLLEARLDAVVYR
                     AKFVPTPWAARQFVNHGHVTVNGKKVNIASYSVKPGDVVEVKEKSRNMALVLEAQQSG
                     ERDIPDYLELGDR"
     gene            complement(672..1571)
                     /locus_tag="VF633_07900"
     CDS             complement(672..1571)
                     /locus_tag="VF633_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8471018.1"
                     /translation="MAGLLALSGCASGPAEAPNLPEPYAARSEVYRFRIGDMEAMALR
                     EGAKHLPNDGKIFGIGQTPETISTALTQARLAGDVIDISFQPLLVRAGDRLVLIDAGS
                     GPTMGPLVGQLAGSLRLAGVDPAEITDIIISHSHPDHAAGLATADGRSAFPNAVIRMS
                     SAEWAFMQVNPALASVISAIKPQVVPLEPGETLAPGIAAVALAGHTPGHIGVRMESAG
                     DALLYIGDVLHHPVISVRHPEWPMVFDDDPAAATSSRRLLMESAARHRALLYAAHFPY
                     PGLGRIEKRCESYVWVPESPVQR"
     gene            complement(1598..2542)
                     /locus_tag="VF633_07905"
     CDS             complement(1598..2542)
                     /locus_tag="VF633_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8471019.1"
                     /translation="MKLIVTSAALAALMLGAAACTPAENAKRPEATEAAAPGSGSTVV
                     ASEGVFPFKIGSLDAIALLDGANPVPNDNQVFGVGRTPEDVAAVLSAADQPTDVMNLS
                     IHPLLIKDGDRTVLIDVGAGAAFGDKAGKLMGNLSAAGVDPASITDVLISHSHPDHIG
                     GLVTAEGALAFPNATIRMSAAEWTFLQANADMAALVTAITPKVQTFAAGAVVAPGITA
                     VSLAGHTPGHTLYEIASGNEKLLYAGDTAHQFVVSLAHPEWEMGYDGDKVAGAKQRQA
                     ELAALTASGERVYFHHFPFPGIGHVQKAAQGYVWVPET"
     gene            complement(2613..3230)
                     /locus_tag="VF633_07910"
     CDS             complement(2613..3230)
                     /locus_tag="VF633_07910"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX8471020.1"
                     /translation="MAFTLPPLPYAYDALEPAIDQETMTFHHDKHHQAYVDNLNKAVD
                     ADASLQGKSLEELFAEISKAPKAVRNNGGGHWNHSLFWELLAPVGSTGEPSAELAAAI
                     ESDLGGLDKFKEDFNAAGAGQFGSGWAWLIVQDGKLKVTSTPNQDNPLMDVAEQKGAV
                     VLAADVWEHAYYLKYQNRRPEYLKAFWTIVNWNKANELYAAAKAA"
BASE COUNT          551 a         1061 c         1089 g          646 t
ORIGIN      
        1 cggtcgccca gttccaggta gtcggggatg tcgcgctcgc cggactgctg ggcttcgagc
       61 accagcgcca tgttgcggga tttttccttg acctcgacga catcgcccgg cttcacggaa
      121 taggaggcga tgttcacctt cttgccgttg accgtgacgt ggccgtggtt gacgaactgg
      181 cgcgcggccc aaggggtcgg gacgaatttg gcgcggtaga cgacggcgtc cagacgagct
      241 tccagcaggc cgatcagggt ctcggaggtg ttacccttcc ggcgagcggc ttcgtcgtag
      301 gtcttgcgga actgcttttc cgtcaggttg gcgtagtagc ccttgagctt ctgcttggcc
      361 ttcagttgca ggccgaagtc ggagaccttg gacttgcggc gttggccgtg ctggccggga
      421 ccgtaggagc gcttgttgac tggcgattta gcgcgacccc acaggttttc acccatggca
      481 cggtcgattt tatacttggc gctgtggcgc ttggacattc gtatctcttc ttcaaggatg
      541 cggcccggcc cctccgaaac tggaagggcg atctcaacgg cggtccacgc atcgtccgta
      601 gtcaattcac gccttcgacc gtgaggccga atgcgggaaa ggcgggctta tgagggtggg
      661 ggccgccgga gtcaacgctg gactggcgat tccggaaccc agacgtagct ctcgcagcgc
      721 ttttcgatgc gtccaagtcc cggatagggg aaatgcgcgg cataaagcag cgcacgatga
      781 cgggcagcgg actccatcaa caaccgtcga ctggacgtcg ccgccgccgg atcgtcgtcg
      841 aacaccatcg gccactctgg atgccgtacc gagatcaccg gatgatgcag gacgtcgccg
      901 atgtagagca gcgcatcgcc ggctgactcc atcctaaccc cgatatgacc cggcgtgtgg
      961 cccgcaaggg caaccgcggc gatcccgggc gccaacgtct ccccgggttc aagcggcacg
     1021 acctgaggct tgatcgcgct gatgacactc gccaatgcgg gattcacctg catgaaggcc
     1081 cattcggccg agctcatgcg gatgaccgca ttaggaaatg cggacctccc atcggctgtc
     1141 gcgagccccg ctgcgtggtc aggatgactg tgcgagatga tgatgtcggt gatctcggcc
     1201 ggatcgacgc ccgccagtct cagcgaccct gccagttgcc cgaccagcgg ccccatggtc
     1261 ggccccgaac ctgcatcgat cagcaccagc cgatcgccgg cgcgaaccag caggggttgg
     1321 aagctgatgt cgatgacgtc tcccgccagt ctcgcctgcg tcagcgccgt gctgatcgtc
     1381 tctggcgttt gcccgatccc gaagatcttg ccgtcgttcg gcagatgctt ggcgccctcg
     1441 cgtagggcca tcgcctccat gtcaccgatc ctgaaccgat agacctcgga cctggcggca
     1501 tagggctccg gcaggttggg cgcttcggcc ggtcccgagg cgcaaccgga caaggccaga
     1561 agtcctgcca tgatcgcgcc tcgggatcgg atagggatca cgtttccgga acccagacgt
     1621 aaccctgcgc cgccttctgc acatggccga tccccgggaa cgggaagtgg tggaaataga
     1681 cccgctcgcc ggacgcagtc agcgcggcca gttcggcctg gcgttgtttg gcgcccgcca
     1741 ccttgtcgcc gtcgtagccc atttcccatt cgggatgggc cagcgacacc acgaactggt
     1801 gcgcggtgtc cccggcgtag agcagcttct cattgcccga ggcgatctcg tacagcgtat
     1861 ggcccggcgt atggcccgcc agcgacacgg cggtgatgcc cggcgccacg actgcgcccg
     1921 ccgcgaatgt ttggaccttc ggcgtgatcg ccgtgactag cgcggccatg tcggcattgg
     1981 cttgcaggaa cgtccactcg gctgcggaca tgcggatggt cgcgttcggg aaggccagcg
     2041 cgccttcagc agtcaccagt ccgccgatgt ggtccggatg gctatgcgag atcagcacgt
     2101 cggtgatcga tgcgggatcg accccggcgg cgctcaggtt gcccatcagc ttgccggcct
     2161 tgtcgccgaa cgctgcgcca gcgccgacgt cgatcaggac cgttcgatcg ccgtccttga
     2221 ttagcagcgg gtggatgctg aggttcatga catcggtcgg ctgatcggcc gcgctcagca
     2281 cggcggcgac gtcttcaggc gtgcggccaa cgccgaacac ctgattgtcg ttcggcacgg
     2341 ggttggcgcc gtccagcagg gcgatggcgt ccaacgaccc gatcttgaac gggaacaccc
     2401 cttccgacgc cacgacggtg gacccggacc cgggcgcggc ggcctcagtg gcttcggggc
     2461 gtttggcgtt ctcggcaggc gtgcaggcgg cggcgcccag catcagcgcg gccaaggcgg
     2521 cgctcgtgac gatcaatttc atggttctgt cctccagtta cgaaaacgcc cgccggagcg
     2581 accggcgggc gtcatatcca gtttctctaa actcaggcgg ccttggcggc ggcgtagagt
     2641 tcgttggcct tgttccagtt cacgatcgtc cagaacgcct tcaggtattc ggggcgacgg
     2701 ttctggtatt tcagatagta ggcatgttcc cagacgtcgg cggccaggac gacggcgccc
     2761 ttctgctcgg cgacgtccat cagcgggttg tcctggttcg gcgtggacgt gaccttcaac
     2821 ttgccgtcct gcacgatcag ccaggcccag cccgagccaa actgaccggc gccggcggcg
     2881 ttgaaatctt ccttgaactt gtcgagcccg cccagatcgc tttcgatcgc ggcagccagc
     2941 tcagccgaag gttcgccggt cgaaccgacg ggtgccagca gttcccagaa caggctgtgg
     3001 ttccagtgac cgccgccgtt gttgcgcacg gccttgggag ccttggaaat ctcggcgaac
     3061 agctcttcca gcgacttgcc ttgcagcgag gcgtcggcgt ccacggcctt gttcagattg
     3121 tcgacgtacg cctgatggtg cttgtcgtgg tggaacgtca tcgtctcctg atcgatcgct
     3181 ggctccagcg catcgtaggc ataaggcagg ggcgggagag tgaaggccat cgtgaggctc
     3241 catgcagtct aggttgacgt caggctcagg agatagtccg cccgcctgcc gaacccaagc
     3301 cgaaccggtc gatttgatca cttttgcgag tcgttcgctg cggcgtc
//
LOCUS       DASVTM010000290         5487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_132500,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000290 DASVTM010000000
VERSION     DASVTM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132500"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="VF633_07915"
     CDS             complement(<1..1742)
                     /locus_tag="VF633_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8471021.1"
                     /translation="MLTVLAVAGLLIVLGLALMRLASVLRDEIGDRTRKTVEVAHSVV
                     AHYHAQQVAGVVTEAQAQQAALSTLKALRYGQEDYFWVTDMHPNMIMHPFNPKLDGTD
                     ISAKKDADGKVMFVAMADVVRTQGEGFVDYRWAKPGQTEASPKISFVKGFKPWGWVVG
                     SGVYTDQIAATVGKAAIGLGSMALLVLAAMATAGWFIGRSVSRPVTALAARMRALAEG
                     DSASPIPGAARADEVGQMAAAVDVFREAAIAKARLEQETETQNRTIEAERLRNQSTGE
                     ALAHEQSLVVAALGEGLDHLKRGDLTYTMEQDFPAHYIKLKTDFNEAMTQLREAMLVV
                     VSNVAGIRAGSSEISQASGDLSHRTEQQAASLEETAAALDEITATVNRTASGARQASE
                     VVQAARGDAEASGVVVRDAVAAMTAIEESSTQISQIIGVIDEIAFQTNLLALNAGVEA
                     ARAGDAGRGFAVVASEVRALAQRSAEAAKEIKTLISTSTSQVGQGVRLVDQTGEALQR
                     IVSRVAEIDGLVSEISASAQEQAVGLQQVNTAVNQMDQVTQQNAAMVEEATAAAAQLR
                     QETGALTRLVARFNV"
     gene            complement(1880..2374)
                     /locus_tag="VF633_07920"
     CDS             complement(1880..2374)
                     /locus_tag="VF633_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX8471022.1"
                     /translation="MAFGIYEASAPVFVRALTNLSAIIDKAVAQGFDETELMQARLAE
                     DMRPFPAQIQLASDAAKGAVARLSGTANPPMADTETSMAELKTRIAATIAFIHSVDRR
                     AFEGAEDREIVLKVPSGEMQFNGAGYLTGFAVPNFFFHVTTAYDLLRHKGVQIGKLDY
                     LGGR"
     gene            complement(2411..3136)
                     /gene="trmB"
                     /locus_tag="VF633_07925"
     CDS             complement(2411..3136)
                     /gene="trmB"
                     /locus_tag="VF633_07925"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX8471023.1"
                     /translation="MPASRPKKPGDPLHPRATGPLRSYGRIKSRMIKPLQAALFDTLL
                     PGITVPDPAAGPIDPKALMPDATEIWLEIGFGGGEHLAAQAASHPQALMIGCEPFLNG
                     VASLLRHIDDGGLTNVRVHTDDARAVVEALPDASLDRIMILFPDPWHKIRHNKRRLIQ
                     DEFAVALARVLKPGGTLRFVTDWKDYAEWALERLDRTPGLVWQANTADDWQVAPDDHV
                     VTRYEEKKLGDTAPLFLEFRRKA"
     gene            complement(3216..4415)
                     /gene="metK"
                     /locus_tag="VF633_07930"
     CDS             complement(3216..4415)
                     /gene="metK"
                     /locus_tag="VF633_07930"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX8471024.1"
                     /translation="MSRSSFLFTSESVSEGHPDKVADRISDTVVDLFLTKDPYARIAC
                     ETLTTTNLVVLAGEIRGEGIMDTAGAWAPGIQDEIEAAVRAAVKDIGYEQAGFHWEKF
                     EFINRLHGQSADIAVGVDAAGNKDEGAGDQGIMFGYASNETPELMPATLQYSHNILRK
                     LAEVRHAGDKRLEPDAKSQVTIEYVDGKPVRATSIVLSTQHAPGLSSADVADIVKPHI
                     LSVLPEGFTDENTVWHINPTGIFEIGGPDGDAGVTGRKIIVDTYGGAAPHGGGAFSGK
                     DPTKVDRSAAYACRYLAKNVVAAGLADRCTIQISYAIGVAKPQSIHVDLHGTGRGVIT
                     EAVLEEKILGLIGGATPRSIREHLGLNKPIYARTSAYGHFGRTPDADGGFSWEKTDLI
                     DQLKALA"
     gene            complement(4473..4931)
                     /locus_tag="VF633_07935"
     CDS             complement(4473..4931)
                     /locus_tag="VF633_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471025.1"
                     /translation="MPPTQLFFLISTLMILPIAWLKGGHPERTGVVVLLLAYLSGVFL
                     QSVRIDRLMIGYAIADLFMLAALIWMTLKYDRWWLFVASAAQALSVLSYLAVLARPEL
                     TARENVGAQWVFGLIALYALLVGVFERWVAGEPPAAPELSDRLGRSRLNV"
     gene            complement(4997..>5487)
                     /locus_tag="VF633_07940"
     CDS             complement(4997..>5487)
                     /locus_tag="VF633_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8471026.1"
                     /translation="RQEQCLRLTAFRTDKEIAAHLGISEATVKKHVHEACQRLGVNRR
                     KAALALLDPTVTDVVEKASIPRVPDYPKQPVARAAVHEHSSLDGAQHVEKGLVEGRTG
                     WPDAALGRRGYRAPPANPLVRIILIIVVTIVLAALVVTLVGMLGDMHEAVGRIDAAST
                     VG"
BASE COUNT          985 a         1710 c         1794 g          998 t
ORIGIN      
        1 acgttaaagc gggcgaccag ccgcgtgagg gcccctgtct cctggcgcaa ctgggcggcg
       61 gcggcggtcg cctcctccac catggccgcg ttctgctggg tcacctggtc catctggttg
      121 acagcggtgt tgacctgctg gagcccgacg gcctgttcct gggccgaggc ggagatttcg
      181 gacaccagac cgtcgatttc ggccacgcgg ctgacgatgc gttgcagggc ctcgccggtt
      241 tggtccacca gccggacgcc ttggccgacc tggctggtgg aggtcgagat gagggtcttg
      301 atctccttgg ccgcttcggc ggagcgctgg gccagggccc gcacctcgga ggcgacgacc
      361 gcgaagccgc ggcctgcgtc gccagcccgg gcggcctcga ccccggcgtt caacgccagc
      421 aggttggtct gaaaggcgat ctcgtcgatc acgccgatga tctggctgat ctgggttgac
      481 gattcttcga tcgcggtcat cgcggcgacg gcgtcgcgca ccacgacgcc cgaggcttcc
      541 gcgtcgccgc gtgcggcctg gacaacctcc gaggcctgac gggcgccaga agcggtgcga
      601 ttgacggtgg cggtgatctc gtcgagggct gcggccgtct cttccagcga ggcggcctgc
      661 tgctcggtac ggtgcgacag atcgcctgag gcctgactga tctcgctaga gccggcgcgg
      721 atgccggcca cgttcgacac gacgaccaac atggcctcgc gaagctgggt catggcttcg
      781 ttgaagtcgg tcttgagctt gatatagtga gccgggaaat cctgctccat ggtgtaggtc
      841 aggtcgccgc gcttcaggtg gtcgaggccc tcgcccaggg cggcgacgac cagcgactgt
      901 tcgtgggcca gagcttcgcc ggtggactgg tttcgcagac gctcggcttc gatggtccgg
      961 ttctgggtct ccgtttcctg ttccagccgt gccttggcga tggcggcctc gcggaacaca
     1021 tcgacggcgg cagccatctg gccgacctcg tcagcgcggg cggcgcccgg aatgggcgag
     1081 gcgctgtcgc cttcggccaa cgccctcatg cgagccgcta gcgccgtcac cggccgcgac
     1141 accgacctac cgataaacca gcccgccgtc gccatggcgg ccagaacaag cagggccatg
     1201 gagccgaggc cgatcgcggc cttgccgaca gtggctgcga tctgatcggt gtagacgccg
     1261 gacccaacta cccagcccca cggcttgaag cccttgacga aggagatttt gggcgaggcc
     1321 tcggtctggc ccggcttggc ccagcgatag tcgacaaagc cttcgccttg ggtgcgcacg
     1381 acatcggcca tggcgacgaa catgaccttg ccgtcggcgt ccttcttggc gctgatatct
     1441 gtgccgtcca gcttgggatt gaacggatgc atgatcatgt tcgggtgcat gtcggtgacc
     1501 cagaaatagt cctcctgccc gtagcggagc gctttcagtg ttgacagggc ggcctgctgg
     1561 gcctgggctt cggtaaccac gccggccacc tgctgggcgt ggtagtgggc gaccacgcta
     1621 tgagccactt cgacggtctt gcgggtgcgg tcgccgatct cgtcgcgcaa cacgctggcc
     1681 agccgcatca gcgccagacc cagaacgatc agcaggcctg ccacagcaag gaccgtcaac
     1741 atgttgacgc gtccgccaat cgagatcgtt ttcataagca cgccgctccc cgttccactg
     1801 cgggtatcgg ccaactgtgt aaagaccggg caaaaaccat aatacgcaca actgcgtacc
     1861 gagcgaagga caagccgctt cagcggccgc cgagatagtc cagcttgccg atctgtacgc
     1921 ctttgtgacg caacaggtcg taggccgtgg tgacgtggaa gaagaagttt ggcacggcga
     1981 agccggtcag atagccggcg ccgttgaact gcatctcacc ggacgggacc ttcaaaacga
     2041 tctcgcggtc ctcggcgccc tcgaacgccc tgcggtccac gctgtggatg aaggcgatgg
     2101 tcgcggcgat gcgggtcttc agttcagcca tgctggtttc agtatcggcc atcggcgggt
     2161 ttgcggtccc gctcagccgc gcgacggcgc ccttggccgc atccgaggcc aattggatct
     2221 gcgccgggaa aggccgcatg tcctcggcca gacgcgcctg catcagctct gtctcgtcga
     2281 atccctgcgc caccgccttg tcgatgatcg ccgacagatt ggtcagggcg cgcacgaaga
     2341 ccggtgcgga agcttcatag atgccgaaag ccatgacgtt agtccttttt caaaactaac
     2401 agtgcttctg ctacgctttg cggcggaact caaggaacag gggggcggtg tcgcccagtt
     2461 ttttttcctc gtagcgggtg acgacatggt cgtccggggc gacctgccag tcatcggcgg
     2521 tgttggcctg ccagaccaga ccgggggtgc ggtcgagccg ctccagcgcc cactcggcat
     2581 agtctttcca gtcggtgacg aagcgcagcg tcccgcccgg cttcagcaca cgcgccagcg
     2641 ccacggcgaa ctcgtcctgg atcaggcggc gcttgttgtg ccggatcttg tgccagggat
     2701 cggggaacag gatcatgatc cggtcgagag aggcgtcggg cagggcctcg accaccgcgc
     2761 gggcgtcgtc ggtgtggact cggacgttcg tcagaccccc gtcgtcgatg tgacgaagca
     2821 gggaggcgac cccattgaga aacggctcgc agccgatcat caacgcctgt gggtgtgagg
     2881 cagcttgcgc cgcgagatgc tcgccgccgc caaaaccaat ctcgagccaa atctcggtcg
     2941 cgtcgggcat gagcgctttc ggatcgatcg ggcctgccgc cgggtcgggc accgtaatgc
     3001 cgggcagcag ggtgtcgaac agcgcagcct gcaggggctt gatcatccgc gacttgatgc
     3061 gcccgtacga ccgtagcggc cctgtggcgc gcggatgcag gggatcaccg ggttttttgg
     3121 ggcgtgaggc aggcatggcg ggggcctata acgcaaaatc gccggccgcg cctctcagcg
     3181 acgaccggcg atcgtttgca gcaaccttca gtcgatcagg cgagggcttt gagctggtcg
     3241 atcaaatcgg ttttttccca gctgaagccg ccgtcggcgt ccggcgtacg gccgaagtga
     3301 ccataggctg aggtacgggc gtagatcggc ttgttgaggc caagatgctc gcggattgaa
     3361 cgtggcgtag cgccgccgat cagacccagg atcttctctt ccagaaccgc ctcggtgatc
     3421 acgccccggc ccgtgccgtg cagatcgacg tggatcgact ggggcttggc cacgccgatg
     3481 gcgtaggaaa tctggatggt gcagcggtca gcgagaccag cggcgaccac gttcttggcg
     3541 aggtagcggc aggcataggc cgccgaacgg tcgaccttgg tcggatcctt gcccgagaag
     3601 gcgccgccgc cgtgtggcgc cgcaccgccg taggtgtcga cgatgatctt gcggccggtc
     3661 acgccagcgt cgccgtcagg tccgccgatc tcgaagatgc cggtcgggtt gatgtgccag
     3721 acggtgttct cgtcagtgaa gccttccggc agaaccgaca ggatgtgcgg cttgacgata
     3781 tcggcaacgt cggcggagga caggcccggc gcatgctggg tcgaaagcac gattgaggtt
     3841 gcgcggaccg gtttgccgtc gacgtattcg atcgtcacct ggctcttggc gtcgggctcg
     3901 aggcgtttgt cgccggcgtg gcgaacttcg gccagcttcc tcaggatgtt atggctgtat
     3961 tgcagggtgg ccggcatgag ctcgggcgtc tcgttcgagg cgtacccgaa catgatgccc
     4021 tgatcgcccg cgccctcgtc cttgttgccc gcagcgtcga cgccgacggc gatgtcggcg
     4081 gactggccgt gcagacgatt gatgaactcg aacttctccc agtggaaacc ggcctgttcg
     4141 tagccgatgt ccttgacggc agcgcgcacg gcggcctcga tctcgtcctg gatgccgggc
     4201 gcccaggcgc cagccgtgtc catgatgcct tcgccgcgga tttcaccagc caacacgaca
     4261 agattagttg tggtcagggt ctcgcacgcg atccgcgcat agggatcctt ggtcaggaaa
     4321 aggtcgacga ccgtgtctga gatgcgatcc gcgaccttgt cgggatgccc ctcggaaacg
     4381 ctttccgagg tgaagaggaa ggaagaacgg gacaaagggc caggctccgg gaacgcagga
     4441 cgcgattttc ggacagacat ataaacatat gcttatacgt tcaagcgaga acgacccaac
     4501 ctgtctgaga gctccggcgc cgcagggggc tcgcctgcga cccaccgctc aaaaacgccg
     4561 accagcaaag cgtatagcgc aataagtccg aacacccatt gagcccccac gttctcgcgt
     4621 gcagtcaact cggggcgtgc cagaacggcg agatatgaca ggacgctcaa ggcctgggcc
     4681 gccgaggcga cgaacagcca ccatcgatca tacttcagcg tcatccagat cagggccgcc
     4741 aacatgaaaa gatcggcgat ggcatagccg atcatcaacc tgtcgatcct gaccgattga
     4801 agaaacacgc ccgacaggta agcgagcaac aacacgacca cccccgttcg ctcgggatga
     4861 ccgcctttga gccaggcgat cggcaggatc atcaaggtcg aaatcaggaa aaatagctgc
     4921 gtcggcggca aggcgctaac tcccgtaagc ggccgcagga aggcctgtca taagcatctt
     4981 ccctgtggag ctccatctat ccgacggtgg aggccgcatc gatcctgcca acggcctcat
     5041 gcatgtcccc gagcatgccc acgagggtta ccaccagagc cgccagaacg atggtcacca
     5101 caatgatcag aatgatccga acgagcgggt tcgccggcgg agcccggtat ccgcgacgcc
     5161 ccaaagctgc gtcgggccag ccagtacgtc cttcgaccag acctttttca acatgttgcg
     5221 ctccatcaag cgaagaatgc tcgtgcacag cagcgcgcgc cacgggttgt ttcggataat
     5281 ctggaaccct cggaatggac gccttttcaa cgacgtctgt tacggtggga tccagaagag
     5341 ccagtgcagc cttgcgtcga tttacgccca gtcgctgaca agcctcgtga acgtgtttct
     5401 tgaccgtcgc ttcagagatg ccgaggtggg cggcgatttc cttgtcggtg cggaaggctg
     5461 tcaggcggag gcattgctcc tgacgcg
//
LOCUS       DASVTM010000291         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_241305,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000291 DASVTM010000000
VERSION     DASVTM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241305"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..599)
                     /locus_tag="VF633_07945"
     CDS             complement(81..599)
                     /locus_tag="VF633_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471027.1"
                     /translation="MKRSLAAVVALLTVTVGACDPNGKAPEASRSEAPGAAVNRQAPQ
                     ADKVASSPPAATSTEGAAMADAIKVGGAPDFAAVYPGGTIEAPPTLAGGPAGPGGLVT
                     FTTDAKPDAVIGFYKQRAEAAGLASVMSMNQGDSLAYGAAEVTNGATLQVVATALEDA
                     PTSVQVTWSAGQ"
     gene            complement(709..1035)
                     /locus_tag="VF633_07950"
     CDS             complement(709..1035)
                     /locus_tag="VF633_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEX8471028.1"
                     /translation="MTDPHLTEDAREADEQGERPDLGRTADALIDALTGTDSGSDTRA
                     GSLQGGSASGSEDDRDQDIINETLASEGMASADPQSPSSVGTGAVATDTDEPGHPEQD
                     PVEGKA"
     gene            1202..1462
                     /locus_tag="VF633_07955"
     CDS             1202..1462
                     /locus_tag="VF633_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471029.1"
                     /translation="MKAAVAKVAPMGATARVKDPASIAADRRAKIGPAEIGDATVNTP
                     DLTRVPTLARSAAWVGAKVAAPEGAAKAGVRVAGVGCSGRAT"
     gene            1441..1890
                     /locus_tag="VF633_07960"
     CDS             1441..1890
                     /locus_tag="VF633_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8471030.1"
                     /translation="MFGPGDLRLVLLALVEQEPRHGYDLIKAIETAFGGGYAPSPGVV
                     YPTLSLLADEGLIAGVEDATGKRVFSVTEAGKTWLDENRAAVDGVMQRMALAAKFVSG
                     EQTPEVVREAFHTLRHAVQMKPGGWTDPETAKVVEALMKAVREISEG"
     gene            complement(1959..>2630)
                     /locus_tag="VF633_07965"
     CDS             complement(1959..>2630)
                     /locus_tag="VF633_07965"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX8471031.1"
                     /translation="YIDRIYDTYIDEQELQNCDHTILEICNRLEPRAYSSREFIWEMG
                     KWLSEGNAKKPGSLIQTAYEEGVPIFCPAFVDSSAGFGLVKHQKERIAAKQPYLMIDA
                     VADFRELTDVKIAAGVTGLFMVGGGVPKNFAQDTVVCAEILGVEAEMHRYAVQITVAD
                     VRDGACSSSTLKEAASWGKVQTTHEQMVFAEATTVVPLIASDAWHREGWKNRDKRRWA
                     KLFGK"
BASE COUNT          454 a          831 c          879 g          466 t
ORIGIN      
        1 gccaaagggg atggtggtac aagcccccag cagggcaacg actgcaatcg cagcgcaggt
       61 cgccctgacc gggatcgcct tcactggccc gcgctccacg tgacctggac cgaggtcggg
      121 gcgtcttcaa gcgccgttgc gaccacctga agagtcgcgc cattcgtcac ctcggcagcg
      181 ccataggcca ggctgtcacc ctggttcatc gacatcacgg aggcgagccc ggcggcctcg
      241 gcccgttgct tgtagaaacc gataaccgca tcgggcttgg cgtcggtggt gaacgtcacg
      301 agcccgccgg gcccggcagg gcctccggca agggtcggcg gagcctcgat cgtgccgccc
      361 ggatagacgg cggcgaagtc cggcgcgccg ccgaccttga tggcgtccgc catagcggcg
      421 ccttccgttg acgtcgcagc gggcggcgac gacgcaactt tatcggcctg gggcgcctgg
      481 cggttcacgg cagcgccggg agcctcgctg cgagaagcct cgggagcctt gccgttcgga
      541 tcacaggcgc caacagtgac cgtcagaagg gcgaccaccg ccgccagact gcgcttcatt
      601 cggtatcgtc ctggaacatt agagtactag gttccaccgt ggcccatcgt tgccgcggcg
      661 gcaaggtgac aacacatccg gcgagaacgg acgcgctttc ggcgaggctt aggccttgcc
      721 ctcgacggga tcctgttcag gatgccccgg ctcgtccgtg tcggtggcca ccgctccggt
      781 tccgaccgaa gacggagact gggggtcagc cgacgccatg ccttcagacg ccaaagtctc
      841 gttaatgatg tcctgatcac ggtcgtcctc cgaaccgctg gccgagccgc cttgcaggga
      901 gcctgcccgt gtgtccgaac cggagtcagt gccggtcagg gcgtcgatca gcgcatccgc
      961 agtacgaccc agatcgggcc gctcaccctg ctcatcggct tctcgggcgt cttcggtcag
     1021 atggggatcg gtcatgggag tctccttacg acccttaaag cctcgcggtc tcagcggggt
     1081 tccgattcat ttaaagatat atctcagagc tcttgcgttg cgccattaaa gatatatctt
     1141 tgcatcatga aaatgaatgg aaacgaaaac atgttcggat tttttgaaga gcggggccgt
     1201 cgtgaaggca gcagtggcca aggtcgcccc catgggcgcc acagccaggg tgaaggaccc
     1261 ggcgagcatc gctgcggacc gtcgggcgaa gatcggaccg gcggagatcg gggacgccac
     1321 ggtcaacacg ccggatctta cacgggtccc cacgctggcc cgttcggccg catgggtcgg
     1381 ggcgaaggtc gcggcgccgg agggcgcggc gaaggccggg gtccgggtcg cgggcgtcgg
     1441 atgttcgggc cgggcgacct gaggctggtg cttctggcgc tggtcgaaca ggagccgcgt
     1501 cacggctacg acctgatcaa ggccatcgag acggcgttcg ggggcggata tgcgccttcg
     1561 cctggcgtcg tctatcccac gctttcgctg ctagccgacg aaggtctgat cgccggggtc
     1621 gaggatgcta cgggcaaacg cgtcttctcc gtcaccgagg caggcaagac ctggctggac
     1681 gagaaccgcg ctgcggtgga cggcgtcatg caacgcatgg ccctggccgc gaaattcgtt
     1741 tcaggcgaac agacgccgga ggtggtccgc gaagcgttcc acacgctgcg ccacgccgtg
     1801 cagatgaagc ccggcgggtg gacggacccc gagaccgcca aggtcgtcga ggctctaatg
     1861 aaagctgtgc gggagatcag cgaaggctga gatcacgatg caaaaagaag gggccgcgat
     1921 cactcgcggc ccctttttca tgtctgcagt aggtggtctt acttcccgaa cagcttggcc
     1981 cagcggcgct tgtcgcggtt cttccagcct tcgcggtgcc aggcgtcgga cgcgatcagc
     2041 ggcacgacgg tggtggcttc ggcgaagacc atctgttcgt gtgtcgtctg gaccttgccc
     2101 cacgaggccg cttccttgag cgtcgatgac gagcaggcgc cgtcgcggac gtcagcgacc
     2161 gtgatctgga ccgcatagcg gtgcatctca gcctcgacgc cgaggatctc ggcgcagacg
     2221 acggtgtcct gcgcgaagtt cttgggcacc ccgccgccga ccatgaacag gccggtgacg
     2281 cccgcagcga tcttgacgtc ggtcagctcg cggaagtcgg cgaccgcatc gatcatcaga
     2341 tagggctgct tggccgcgat ccgctccttc tggtgcttga ccagaccgaa gcccgccgag
     2401 ctgtcgacga acgcggggca gaagatcgga acgccttctt cataggcggt ctggatcagc
     2461 gagccgggct tcttcgcgtt gccctcgctc agccatttgc ccatctccca gatgaactcg
     2521 cggctcgaat aggcgcgcgg ctccagccgg ttgcagatct ccaggatcgt atggtcgcag
     2581 ttctgcagct cctgctcgtc gatataggtg tcgtagatcc ggtcgatgta
//
LOCUS       DASVTM010000292         5496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_827192,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000292 DASVTM010000000
VERSION     DASVTM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827192"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VF633_07970"
     CDS             complement(<1..1222)
                     /locus_tag="VF633_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HEX8471032.1"
                     /translation="MTARILVVDDVEANVRLLEAKLTIEYYDVLSCTDGLTALKLASA
                     ELPDIILLDVMMPGMDGFETCKRLKANPETSHIPVVLVTALDGREDRIRGLDAGADDF
                     LTKPLDDVVLFARVRSLVRLKLVMDELREREESGRRMGVTTDGAGRLRGSGGRVLIVD
                     DNVRQSSKIVEEMSREHRPVVETDAGAALIASRGLIDLMIVNTVAESFDGLRLIAQVR
                     SAETTRHLPILAIVDPGQRPRLLKALELGVNDILAKPVDPEELAARARTQIRRKRYTD
                     FLREKLDYSLEMAVTDALTGLHNRRYMSGQLQAFVQRANHGGDPVAVLVLDIDHFKSV
                     NDGFGHDAGDEVLSEFAVRLATNVRAVDLPCRLGGEEFVVVMPDTTLEDAHRIADRIR
                     RDIGQAPFRVMGGKE"
     gene            complement(1219..1608)
                     /locus_tag="VF633_07975"
     CDS             complement(1219..1608)
                     /locus_tag="VF633_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471033.1"
                     /translation="MSKKVLIVEDNELNMKLFHDLLDSQGYETLQTREGLQAMALARL
                     HMPDLILMDIQLPEISGLEVTKWLKDDEELAHIPVIAVTAFAMKGDEERIRQGGCEAY
                     ISKPISVMHFLETVRKHLGAPAEVVRG"
     gene            1755..3020
                     /locus_tag="VF633_07980"
     CDS             1755..3020
                     /locus_tag="VF633_07980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX8471034.1"
                     /translation="MQRAAIQAICRECLWTGSEPANRCGSCGSVRVVSDPELDQLTIA
                     HLDCDAFYASVEKRDRPELRDKPVIVGGGVRGVVSTCCYVARMYGVHSAMPMFKALKA
                     CPDATVIKPDFSKYVFESQRIFGFVGELTPLVQTLSLDEAWIDLAGTERLNGGPPALQ
                     LIRLMKRIEDETGLTVSIGLAPNRFLAKMASEMDKPRGFSVISRRDAVSILGPRPVTA
                     LPGVGPVFGKTLRSDGYNTIGDLAAADVKTLVKRYGEGGLRLYDLSHARDARPVRPDH
                     DRKGMSAETTFNTDLTTAEELDAELWPLCEKLASKARRDGVSSRSVVLKLRRADFKII
                     TRRHTVPEPIQTARALFALGRELLKPELGIPYRLIGIGMADVQDAVEGPSTLFASTET
                     RVLKTETTIDKLRARFGADAVFAGRALKR"
     gene            3065..3802
                     /locus_tag="VF633_07985"
     CDS             3065..3802
                     /locus_tag="VF633_07985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8471035.1"
                     /translation="MSAFIDKMRRRAGRYLDVRPVEVAPERGVFSLSFDDIPRTAWTE
                     AGPILAEHGILATYYVCGGLSDGSNMDLPQFSTDDLLELHEAGHEVGCHTYEHVSTLT
                     LSPAELDASLARNAAWVGERLDDYVMRTFAYPFGDASLGSKAVVDSRFLCGRGVRDGI
                     NAGKADRAALQAIGLESRRLPGYDLEALMAKTAEDRGWLIAYGHDVMDSPTPYGCTPD
                     DLDRVIRLAKAADLEILPVAAAWDRVR"
     gene            complement(4330..>5496)
                     /locus_tag="VF633_07990"
     CDS             complement(4330..>5496)
                     /locus_tag="VF633_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471036.1"
                     /translation="ALTVGAFGIGVTEFVIMGLLLQVSNDLGVGLPAAGLLMTGYALG
                     VFVGAPVLTIATRRLPRKTTLLVLMAIFTLGNLACALAPSYPILMTARIVTALAHGTF
                     FGVGSMMAASLVAPDKKASAIALMFTGLTLATLLGVPFGAWLGVAYGWRATFWAVAGI
                     GVLALVALAMFLPKIPAETPSPLREELAVLARPQVLMGLAMTVLGFGGVFAVFTFIQP
                     ILTELSGFSTAAVSPILLVFGAGLVVGNLAGGKLADKKLGPALLGSIALLTLAMAIAG
                     FAFQSQVGAIAVTFLLGFAAFATVAPLQMRVLQQAGGAGQTLASSLNIAAFNLGNALG
                     AWLGGAILLAGLGLAAIPLIAAVVPLAALLLAGLSLWLEKREGGGASLSRGERA"
BASE COUNT         1007 a         1903 c         1684 g          902 t
ORIGIN      
        1 gttccttccc tcccatgacc cggaacggcg cctgtccgat gtcgcggcgg atgcggtcag
       61 cgatacgatg cgcgtcctca agggtggtgt cgggcatcac cacgacgaac tcctcaccgc
      121 ccagacgaca cggcagatcg acggcgcgga cgttcgtagc aagccgtacg gcgaactcgc
      181 tcagaacttc atccccggca tcatggccga aaccgtcatt gaccgatttg aagtggtcga
      241 tatccagcac cagcaccgcg acgggatcgc cgccgtggtt ggcgcgctgg acgaaggcct
      301 gaagctggcc ggacatgtat cggcgattat gcaggccagt cagggcgtcg gtgaccgcca
      361 tctcgaggct gtagtccagc ttctcgcgca ggaagtcggt atagcgtttg cgcctgatct
      421 gcgtccgcgc cctcgccgcc agttcttccg gatcgacagg tttggccagg atgtcgttga
      481 cgccgagctc cagcgccttg agcagccggg gccgctggcc gggatcgaca atggcgagaa
      541 tgggcaggtg tcgcgtcgtc tcggccgagc gcacctgcgc gatcagcctc agcccatcaa
      601 agctctcggc gaccgtgttt acgatcatca ggtcgatcag gccgcgtgag gcgatcaggg
      661 ctgcacccgc atcggtctca accaccggcc ggtgttcgcg cgacatctcc tctacgatct
      721 tgctcgattg ccggacgttg tcgtcgacga tcagaacacg accgcccgaa ccccgcagcc
      781 gcccggcgcc gtcggttgtg acgcccatgc gacgaccgct ttcctcgcgc tcacgcagct
      841 cgtccatcac cagcttgagc cggaccagcg accgcacgcg ggcgaacagc accacatcgt
      901 cgagcggctt ggtcaggaag tcgtcggcgc cggcgtcaag gccgcgaatg cggtcctcgc
      961 gaccatccag cgcggtgacg agaaccaccg ggatgtggct cgtttcggga ttggccttga
     1021 gccgcttgca cgtctcgaac ccgtccatgc ccggcatcat gacgtccagc aggatgatat
     1081 ctggcagttc ggcagaggcc agtttcaggg ccgtcagccc gtcggtgcag ctcagcacgt
     1141 cgtaatattc gatcgtcagc ttggcttcga gcagcctgac gttggcctcg acgtcgtcga
     1201 cgaccagaat acgagccgtc atccccgaac cacctcagca ggcgcgccca gatgcttgcg
     1261 cacggtttcg aggaaatgca tcaccgagat cggcttggag atataggcct cgcagccgcc
     1321 ctggcggatg cgctcctcgt cgcccttcat agcgaatgcg gtgacggcaa tgaccggaat
     1381 gtgcgccagt tcctcgtcgt ccttcagcca tttggtgact tcgagacccg agatttccgg
     1441 aagctgaatg tccatcagga tcagatcggg catgtgcaga cgcgccaaag ccatcgcctg
     1501 caacccttcg cgggtctgca gggtctcata gccttgcgag tcgagcagat catgaaacag
     1561 cttcatgttc agctcgttat cctcgacgat gaggactttc ttggacatca tcacccggtc
     1621 tgtctggcct tgaaaatcgc gcggcttgcg ctgaggcttt tcgccttctt cgagtgcaat
     1681 atgcgcgcgc cagtcttaag ttggcgtgaa gcccgcgcga accgcgccgg gccatctttc
     1741 ggggctcctc ggcaatgcag cgcgcggcca ttcaggccat ctgtcgggaa tgcctgtgga
     1801 ccggcagcga accggcgaac cgctgtggat cgtgcggctc ggtgcgtgtg gtgtccgatc
     1861 ccgagctcga ccagctgacc atcgcccacc tcgactgcga cgccttctac gcctcggtcg
     1921 agaagcgcga ccggcccgaa ctccgtgaca agccggtgat cgtcggcggc ggcgtacgcg
     1981 gcgtcgtctc gacctgctgc tatgtcgcgc gcatgtacgg cgtccattcc gccatgccga
     2041 tgttcaaggc gctcaaggcc tgtcccgacg cgacggtgat caagccggat ttcagcaaat
     2101 acgtgttcga atcccaacgc atcttcggct tcgtcggtga actgacgccg ttggttcaga
     2161 ccctgtcgct ggacgaggca tggatcgatc tggcgggcac cgaacgcctc aacggtggtc
     2221 cgccggccct gcagctgatc cggctgatga agcggatcga ggacgagacc ggactgacgg
     2281 tctccatcgg cctggccccc aaccggttcc tcgcgaagat ggcgtccgag atggacaagc
     2341 cccgcggctt ctcggtgatc tcaaggcgtg acgcggtgtc gatcctcggc ccgcgcccgg
     2401 tcaccgccct gccaggcgtc ggtccggtgt tcggcaagac cctgcgcagc gacggttaca
     2461 acaccatcgg cgatctcgcc gccgccgacg tcaagactct ggtcaaacgc tatggcgagg
     2521 gcggtctgcg gctctatgat ctcagccacg cccgcgacgc gcgcccggtc cgcccggacc
     2581 atgatcgcaa ggggatgagc gccgaaacca ccttcaacac cgacctgacc accgccgagg
     2641 aactcgacgc cgaactctgg cccctgtgcg agaaactggc gtccaaggcg cgtcgcgacg
     2701 gggtgtccag ccgatcggtc gtgctcaagc tgaggcgggc cgacttcaag atcatcacgc
     2761 gccgccacac ggtccccgag ccgatccaga ccgcccgcgc cctgttcgcc ctcggccgtg
     2821 aactattgaa gccggaactg ggtatcccct atcgtctgat cggcatcggc atggccgacg
     2881 ttcaggacgc cgtcgaaggc ccctccacat tgttcgcctc gaccgagacg cgtgtgttga
     2941 agaccgaaac caccatcgac aagctgcgtg ccagattcgg tgccgacgcg gtgttcgccg
     3001 ggcgagcgct gaaacgctga ggccgttcaa aggaccttaa cagcgaccgg ttatattacc
     3061 gctcatgtcc gcattcatcg acaagatgcg ccgccgcgcc ggccgctacc tcgacgtccg
     3121 gccggtggag gtcgcgcctg agcgtggcgt cttcagcctg tcgttcgacg acattccgcg
     3181 cacagcctgg accgaagccg gaccgatcct ggccgagcac ggcatcctcg cgacctacta
     3241 tgtctgcggc ggcctgtcgg acgggtcgaa catggacctg ccccagttct cgaccgacga
     3301 cctgcttgaa ctgcacgagg ctggccacga ggtcggctgc cacacctatg agcacgtctc
     3361 gaccctgacc ctgtcccccg ccgaactgga cgccagccta gcccgcaacg ccgcatgggt
     3421 tggcgagcgg ctcgatgact acgtcatgcg caccttcgcc tatcccttcg gcgacgcatc
     3481 gctgggctcc aaggccgtgg tcgacagccg cttcctgtgc ggacgcggcg tgcgcgacgg
     3541 catcaacgcc ggaaaagccg accgcgccgc cctgcaggcc atcggtctgg agagccgccg
     3601 cctccccggc tacgacctcg aagctctgat ggcgaagacc gccgaggatc gcggctggct
     3661 gatcgcctac ggccacgacg tcatggacag ccccaccccc tacggctgca ccccagacga
     3721 cctcgaccgc gtcatccgcc tcgccaaggc cgccgatctt gagatcctgc cggtcgccgc
     3781 ggcgtgggac agagtccgat aacctcccct ctcccggcgg gagagggctt gagcgcacga
     3841 gagcgcagcg atcgtcctcg ggcgaaaggg tgaggggcta aaccaaccgg tccacgccat
     3901 gaacccccac atcgtcgccg atcaacatcc tgatcagcca cgacttcctc tgcaaacacg
     3961 ccattccatc cagcatcatc actcgccgac ctatactgcg cgcccggtct gtgacatccc
     4021 gcgctcagcc attcagacgt cgcacccgat tggactccga gtccaatcca ccggctctga
     4081 cccagcgcta tagattcagt gagttgttca ccaactctca cccttaacct tcaattctgg
     4141 acgttttgga ctctgttttt tttgagtcca atccccaccg tcctatcctg tcccggcggg
     4201 agagggcttg agcctccggg agcgaagcgc tcggtcaggc aaaagggtga ggggtcaatg
     4261 tcggaggttt gcgcgaaagg gcgcccctca ccctctcgcg caaggacgac ggcttgcgcc
     4321 gccgtgcgct caagccctct ccccacggga gagggaagcg cccccgccct cccgcttctc
     4381 aagccacaaa ctcaaccccg ccagcaacag cgcagccaac ggcacgaccg ccgcgatcag
     4441 cgggatggcg gccagtccca gaccggcgag caggatcgct ccgcccagcc aggcgcctaa
     4501 ggcgttgccg aggttgaagg cggcgatgtt caggctggag gccagcgtct ggcccgcacc
     4561 gccggcctgt tgcagcaccc gcatctgcag cggcgcgaca gtggcgaagg cggcgaagcc
     4621 cagcaggaag gtcacggcga tcgctccgac ctgactttgg aaggcgaagc cggcgatggc
     4681 catggcgagg gtcagcagcg cgatactgcc cagcagcgcc ggacccagct tcttgtccgc
     4741 cagcttgccg cccgcgagat taccgacgac cagaccggcg ccgaacacca gcaggatcgg
     4801 cgaaacggcg gcggtggaga agccggacag ttcggtcagg atcggctgga tgaaggtgaa
     4861 gacggcgaac accccgccaa agcccagcac cgtcatggcc agacccatca gcacctgcgg
     4921 acgcgccagc acggccagtt cctcacgcag gggcgacggc gtctcggccg ggatcttcgg
     4981 caggaacatc gccagtgcaa ccagcgccag cacgccgatc ccggccacgg cccagaaggt
     5041 cgcgcgccag ccgtaagcga cgcccaacca ggccccgaac ggcacgccca gcagcgtcgc
     5101 cagcgtcagg ccggtgaaca tcagggcgat ggcgctggcc ttcttgtccg gcgcgacgag
     5161 gctggccgcc atcatcgatc cgacgccgaa gaaggtaccg tgagcgaggg ccgtgacgat
     5221 ccgggccgtc atcaggatgg gataagacgg cgccagcgca caggccagat tgcccagcgt
     5281 gaagatggcc atcagcacca gcagcgtggt cttccggggc aaccgccgcg tagcgatggt
     5341 cagcaccggc gcgccgacga acacgcccag cgcatagccg gtcatcagca acccggcggc
     5401 aggcaggccc acaccgaggt cgttcgagac ctgcagcagc aggcccatga tgacgaactc
     5461 cgtgacgccg atcccgaaag ctccgacggt cagggc
//
LOCUS       DASVTM010000293         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_774368,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000293 DASVTM010000000
VERSION     DASVTM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774368"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /gene="kduI"
                     /locus_tag="VF633_07995"
     CDS             <1..542
                     /gene="kduI"
                     /locus_tag="VF633_07995"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEX8471037.1"
                     /translation="TFTLKPKDGLYIPMGSAEVSFAGETRFYLVSTPAHAAYEVQHIS
                     IDQAISMERGSLETSNERTIYQYIIPGVCRSCQLVMGLTMLKPGSVWNTMPPHVHERR
                     SEAYFYFDLGAENRVYHFMGKPDAQRHIVIADSEAVVSPPWSIHMGAGTSNYTFIWAM
                     GGENVDYTDMNVLDICQLK"
     gene            549..1307
                     /gene="kduD"
                     /locus_tag="VF633_08000"
     CDS             549..1307
                     /gene="kduD"
                     /locus_tag="VF633_08000"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEX8471038.1"
                     /translation="MTNPFSLSGKVALVTGGNVGIGQGIALALADAGADIASVARRTS
                     DDTVGKVLAKGRRAISIQADLMSMEPIGRIIEEAEKGLGPIDILINNAGIIRRNDAVD
                     FTEQDWDDVMNVNLKTVFFLSQAVVKTMIAGARGGKIVNTASMLSYQGGIRVPSYTAS
                     KSGVAGLTKLMANEWAKHGVNANAIAPGYFATDNTQALQDDPVRNKEILGRIPAGRWG
                     DPSDIGGAAVFLSSSASDYVQGITLPVDGGWLAR"
     gene            1329..2360
                     /locus_tag="VF633_08005"
     CDS             1329..2360
                     /locus_tag="VF633_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX8471039.1"
                     /translation="MTAKKILCFGEMMLRLNPPEGEVLMQGNSLTVRPGGAEANVAVS
                     LARYGAPTAMATVLPDNALGYGTRDELRKHGVDTSGIVFKPGRLGLYFMTPGAVRRPA
                     DVLYDRADSAFVKHVDTAFDWPALLDGVEWLHVSGVTPAVGPTGTRAAIQAVDAAKAA
                     GVKVSFDGNFRGKLWSEWAGDPPKTLGHMLSTASIAFADERDFALVLGRTFDSADPHE
                     RRRAAAAAAFEAFPTLERIACTLRVQESVADQALSAIMLTRSGAEVVETHAEPIHMSG
                     VIDRVGGGDAFASGVLFGVWSGWSDDEALAFGLAAAGLKHSVHGDFNLFTAEDVRAAM
                     GRGGFDIRR"
BASE COUNT          483 a          932 c          840 g          474 t
ORIGIN      
        1 aaaccttcac gctgaagccg aaggacggcc tctacatccc catgggctcg gccgaggtca
       61 gcttcgcggg cgagacgcgg ttctatctgg tctcgacgcc cgcccacgcg gcgtatgagg
      121 tccagcatat ctccatcgac caggccatct cgatggagcg cggctcgctg gagacctcca
      181 acgagcggac catctaccag tacatcatcc cgggcgtctg tcggtcctgc cagctggtca
      241 tgggcctgac catgctgaag ccaggcagcg tctggaacac catgccaccg catgttcacg
      301 aacgccggtc ggaggcctat ttctacttcg atctgggcgc agagaaccgc gtctatcact
      361 tcatgggcaa gcccgacgcg cagcggcata tcgtcatcgc cgacagcgaa gcggtggtca
      421 gcccgccctg gtcgatccat atgggcgcag gcacctccaa ctacacattc atctgggcga
      481 tgggcggcga gaacgtcgac tataccgaca tgaacgtgct cgatatctgt cagttaaagt
      541 aggccttcat gaccaatccc ttcagcctgt ccggcaaggt cgcgctcgtc accggcggta
      601 acgtgggcat cggtcagggc atcgccctcg cccttgccga tgccggcgcc gacatcgcct
      661 cggtggcgcg ccgcacgtcc gacgacaccg tcggcaaggt cctcgccaag ggccgtcgcg
      721 cgatctcgat ccaggctgac ctgatgtcga tggagcccat cggccgcatc atcgaggaag
      781 ccgagaaggg ccttggcccg atcgacatcc tgatcaacaa cgccggcatc atccggcgca
      841 acgacgcggt cgacttcacc gaacaggatt gggacgacgt catgaacgtc aatctcaaga
      901 ccgtcttctt cctcagccag gccgtggtga agaccatgat cgcaggcgca cgcggcggga
      961 agatcgtcaa cacagcctcg atgctgtcct atcagggcgg catccgcgtg ccgtcctaca
     1021 ctgcgtccaa gtcgggcgtc gccggcctaa ccaagctgat ggccaacgag tgggccaagc
     1081 atggcgtcaa cgccaacgcc atcgcgcccg gctatttcgc cacggacaat acccaggcgc
     1141 tgcaggacga ccccgtacgc aacaaggaaa tcctcggccg catccccgcc gggcgttggg
     1201 gcgatccgtc cgatatcggc ggcgctgccg tgttcctctc cagctcggcc agcgactatg
     1261 ttcaaggcat caccctgccc gtcgacggcg gctggctcgc gcgctgatcc cgcgctagcg
     1321 ttaccgatat gactgctaaa aaaatcctct gcttcggcga gatgatgctg cgcttgaacc
     1381 cgcccgaggg tgaggtgctg atgcaaggca acagcctgac cgtgcgtccc ggcggggccg
     1441 aggccaatgt cgccgtatcg ctggcccgct acggcgcacc gacggcgatg gcgaccgtcc
     1501 tgcctgacaa tgcgctgggc tacggcacgc gcgacgaact gcgcaaacac ggcgtcgata
     1561 cttcgggcat cgtgttcaag ccgggtcgcc taggtctgta tttcatgacg ccgggcgccg
     1621 ttcgccgtcc cgccgacgtg ctctatgacc gcgccgactc agcgttcgtg aagcatgtcg
     1681 acaccgcctt cgattggcct gccctgctgg acggcgtcga atggctgcac gtctcgggcg
     1741 tgacccccgc cgtcggcccg accggcaccc gcgccgcgat ccaggccgtg gatgccgcca
     1801 aggctgcagg cgtgaaggtc tccttcgacg gcaatttccg cggcaagctg tggagcgaat
     1861 gggccggcga tccgccgaag acgctcggtc acatgctgtc caccgcctcg atcgcctttg
     1921 ccgatgagcg cgactttgcg ctggttctgg gccgcacctt cgacagcgcg gatccgcatg
     1981 agcgccgccg cgccgcagcc gccgcagcct tcgaagcctt cccgacgctg gagcgaatcg
     2041 cctgcaccct gcgggttcag gaaagcgtcg cggatcaggc cctgtcggca atcatgctta
     2101 cgcgttcggg agcagaggtc gtcgagaccc acgccgagcc gatccacatg tccggcgtca
     2161 tcgaccgcgt cggcggcggc gacgccttcg cctctggcgt gctgttcggt gtctggagcg
     2221 gctggtccga cgacgaggcg ctggcgttcg gtctggcggc ggcgggtctc aagcactcgg
     2281 tgcatgggga cttcaacctg ttcaccgccg aagatgtgcg cgcggcgatg ggccgcggcg
     2341 ggtttgatat acggcgatag gactgccctt ctccccttgc gggagaaggt ggcgcgcgaa
     2401 gcatgacgga tgaggggtcg tgccgttgac ttgagtgagc gatccctagg cgtcagacgt
     2461 cagcgaaacc cctcatccgt cagcctgcgg ctgccacctt ctcccgcaag gggagaagga
     2521 taggctcgca ttcccacatc acgccgccat ctgaaaaatc gctcctgacc tccctaggaa
     2581 accggtgtca tcggtgctta atctgctgcg tcaaagggcc gtaagagtcc tcaccctggg
     2641 aagctggaaa caccgaacgc catgtctatg aaccgccgcg cccttctgtc cgccgcagcc
     2701 gcgaccgccg tcgcctcggc cacgcccgc
//
LOCUS       DASVTM010000294         5052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_188944,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000294 DASVTM010000000
VERSION     DASVTM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188944"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /gene="ispH"
                     /locus_tag="VF633_08010"
                     /gene_synonym="lytB"
     CDS             <1..354
                     /gene="ispH"
                     /locus_tag="VF633_08010"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921190.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX8471040.1"
                     /translation="DICYATTNRQEAVKVLADGCDLVLVVGSPNSSNSVRLVEVALRG
                     GARDAHLVDDASQVDWRWFDGVNTVGVTAGASAPEPLIQALIAAIADRFDTTMINDPG
                     ERETVTFKLPRALTA"
     gene            complement(351..974)
                     /gene="scpB"
                     /locus_tag="VF633_08015"
     CDS             complement(351..974)
                     /gene="scpB"
                     /locus_tag="VF633_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX8471041.1"
                     /translation="MERRVEALLFAAAGPLSAAEIARRLPEGTDVGAAISGLRKRYEG
                     RGVELECVADRWRFRTAPDLAFLMTEEREEPRRLSKAALETLAIIAYHQPVTRAEIES
                     VRGVSISRGTLDLLLEMGFVRLRGRRRTPGRPVTYATADRFLEHFALASLHDLPGSAE
                     MKAAGLLDLSLPNGFEVPDPSRSGDTDDEDPIDNSDSPEFAQDFVGD"
     gene            complement(1007..1843)
                     /locus_tag="VF633_08020"
     CDS             complement(1007..1843)
                     /locus_tag="VF633_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX8471042.1"
                     /translation="MTEAFQPNLDFTASQGFGEQMEPGEAFVVDLDGYEGPLHVLLAL
                     ARNQKVDLLKLSITKLAEQYLAFVHEARRRNFSLAADYLVMASWLAYLKSRLLLPRTE
                     KGKGDEPPAEEMAAALAFRLQKLEAMRKAVEAIQARPQLKRDVFTRGDPEATVIVPSD
                     RIDASLYELMSAYVVQRRREQSRHYTPGQRVEAFGLEAAREWLRDVLPRLGEWTPLEK
                     VAPVYDDGAEGPNQASFTASTLSASLELVKEGAMDVRQDGAFEDIYLKRRGEGFALEL
                     TS"
     gene            complement(1852..2961)
                     /gene="nagZ"
                     /locus_tag="VF633_08025"
     CDS             complement(1852..2961)
                     /gene="nagZ"
                     /locus_tag="VF633_08025"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX8471043.1"
                     /translation="MTSAAIYGCSGHVLTDEEREFFADVRPWGFILFRRNVDTPEQVK
                     ALVADLRASIDDPNAPILIDQEGGRVQRLGPPHWPKYPPGSTYLAATDDSATARELVR
                     LGARLMAHDLKALGINVDCVPVLDVPVPGAHDIIGDRAYAQDPATVAQLGRAAAEGLL
                     TGGVLPVIKHIPGHGRAFADSHKDLPVVHADFATLDSWDFAPFKAMSDMPMAMTAHVV
                     YDAIDPRRPATTSKKAVRMIRDHLGFDGLLMTDDLSMKALSGSLTDRAQAALEAGCDV
                     VLHCNGDLDEMKSVAKGVGKLKGKAKARAAAALARIVHTPEPLDAVEARARLDAALIG
                     RLDAAKGPDVGEAQAVSISEASASSASASSSATAR"
     gene            complement(2966..3757)
                     /locus_tag="VF633_08030"
     CDS             complement(2966..3757)
                     /locus_tag="VF633_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8471044.1"
                     /translation="MSYDDNNRPNDSRDRGPYTPPTDDDLPFNRGGFDARRSPPAKAP
                     PITLIVSAVVLLVLIVAVVLFYRSGLRASTDVPPAVGTPVGELKVQAPVEAQPVDEEA
                     GVDVYRNETPSAVTSPTYTAPPEQALPRPAPKPLPPVASDELAPTRPAPTRPTPARPV
                     TPSAPVVRPSPQATQTPPTSAPATGGSAVQIGAFSSTAIADREFAATAARFPQFARGS
                     QKRVQEVTASNGSTVYRTTFYGLSSERAQAFCSAIQSAGGDCLVR"
     gene            complement(3831..4967)
                     /locus_tag="VF633_08035"
     CDS             complement(3831..4967)
                     /locus_tag="VF633_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX8471045.1"
                     /translation="MVPEPPSRTRNAFARDRDRIIHSTAFRRLKGKTQVFVAHEGDHY
                     RTRLTHSLEVAQIARSLAHALGLDQDLAETIALAHDLGHPPFGHAGEDELQVQMKPYG
                     GFDHNVQTFRVVTKLEERYPEFDGLNLSWETVEGVVKHNGPVTHKLDEPAWQAIADYT
                     RTSDNPGWDLRLNTFASLEAQAAAIADDIAYNNHDVDDGVQAGLFTLADLAEVPLIGP
                     VLKGVRADWPELDERMVRLEAVRRMIGVMVDDVLAETARRLEADKIVTPEDVRMAKRT
                     LIQFSDGMLADLGALRIFLFERMYRHYRVNRTRSQARRILAEMFQLFMAEPEVLPPEW
                     SDRASSSDLHKRARAVCDYIAGMTDMYAIEEHRKLFKLDLGREL"
BASE COUNT          846 a         1648 c         1690 g          868 t
ORIGIN      
        1 gacatttgct acgcgaccac caaccgtcag gaagcggtga aggtgctggc cgacggttgt
       61 gatctggtgc tggtggtcgg ctcgccgaac tcctcgaatt ccgtgcgact ggtcgaggtg
      121 gccctgcgtg gcggagccag agacgcgcat ctggtcgatg acgcctcgca ggtcgactgg
      181 cgctggttcg acggcgtgaa caccgtcggc gtcaccgccg gcgcctcagc ccccgagccg
      241 ctgatccagg cgctgatcgc cgccatcgcc gaccgcttcg acaccacgat gatcaacgat
      301 ccgggcgagc gggagacggt gacgttcaag ctgccgcgag cactgacggc ttagtcgccc
      361 acgaagtcct gagcgaactc ggggctatcg ctgttgtcga tcgggtcttc atcgtcggtg
      421 tccccactac gtgaggggtc gggcacctca aagccgttag gcagggatag gtcgagcagg
      481 cctgccgcct tcatctctgc ggagccgggc agatcatgca ggcttgctag ggcgaaatgt
      541 tctaggaagc ggtcggcggt ggcgtaggtc accgggcgac ccggcgtccg gcggcgtccg
      601 cgaagccgga cgaagcccat ctccagcaac aggtcgagcg tgccgcgcga gatcgaaacg
      661 ccccgcacgc tctcgatctc cgcgcgggtg acgggctggt gataggcgat gatggcgagt
      721 gtctcgagcg cggccttgga caggcggcga ggctcctcgc gctcctcggt cattaagaac
      781 gctagatcag gcgcggtgcg gaaacgccag cggtcggcga cgcattccaa ctccaccccc
      841 ctgccctcat agcgtttgcg aaggcccgag atggcggcgc ccacatcggt cccttcgggc
      901 agccggcggg cgatctcggc ggctgacagc ggcccggcgg cggcgaacag caacgcctcg
      961 acccggcgtt ccatctcgga ttcatccggg gcgaacctga gatcggtcac gaggtgagtt
     1021 ccagtgcgaa tccctcgcct cgtcgcttga gatagatgtc ctcgaacgcg ccgtcctgac
     1081 ggacgtccat cgccccttcc ttgaccagtt cgagactggc cgagagggtc gaggcggtaa
     1141 agctcgcctg gttgggaccc tcggcgccgt cgtcataaac cggagcgacc ttctccagcg
     1201 gcgtccattc ccccaaccgg ggcaggacgt cgcgcagcca ttcgcgagcg gcctccagtc
     1261 cgaaagcctc gacccgctgg cccggcgtat aatggcgcga ctgctcgcgc cgccgctgga
     1321 ccacataggc gctcatcagc tcatacaggc tggcatcgat acgatcagag ggcacgatca
     1381 ccgtcgcctc gggatcgccc cgggtgaaga cgtcgcgctt cagctgcggc cgggcctgaa
     1441 tggcctccac cgccttgcgc atcgcctcaa gcttctgcag cctgaacgcc agcgccgccg
     1501 ccatctcctc ggcaggaggc tcgtcgccct tgcccttctc agttcgcggc agcagcaggc
     1561 gcgacttcag gtacgccagc caggacgcca tcacgagata gtccgccgcc aacgagaaat
     1621 ttcgtcgccg ggcctcgtgc acgaaggcca gatactgttc ggccagcttc gtgatcgaga
     1681 gcttgagcag gtctaccttc tggttcctcg ccaaggccag cagcacgtgc agcggcccct
     1741 cgtagccgtc cagatccacc acgaacgcct cgcccggctc catctgctcg ccaaagcctt
     1801 gggaggcggt gaagtccaga ttgggctgga aggcttcggt catccttcag gctaccgcgc
     1861 ggtcgcgctg cttgaggccg aggcggatga tgcagaggct tcggagatgc ttacggcctg
     1921 cgcctcgcct acgtcagggc ccttggcggc gtccagccga ccgatcaacg ccgcatcgag
     1981 ccgggcacgc gcttcgacgg cgtccagcgg ctcaggcgtg tggacgatgc gggccaaggc
     2041 ggcggcggcg cgggctttgg ccttgccctt cagcttgccg acacccttgg cgaccgactt
     2101 catttcatcg aggtcgccgt tgcagtgcag gaccacatcg caccccgcct cgagcgccgc
     2161 ttgggcccgg tccgtcaaac tgccggagag cgccttcatc gacaggtcgt cggtcattag
     2221 caggccgtcg aagccaagat ggtcgcggat catccgcacc gccttcttcg aggtggtagc
     2281 ggggcgtctg gggtcgatag cgtcgtagac gacgtgggcc gtcatcgcca tcggcatgtc
     2341 cgacatcgcc ttgaacggcg caaaatccca gctgtcgagc gtcgcgaaat cggcatggac
     2401 caccggcaag tccttatggc tgtcggcgaa ggctcggccg tggccgggga tgtgcttgat
     2461 gactggcagg acgccgcctg tcagcaggcc ctcggcggcg gcgcggccca gttgcgccac
     2521 ggtcgctgga tcctgcgcat aggcgcggtc gccgatgatg tcgtgggcgc cgggcaccgg
     2581 cacgtcgagc actggaacgc agtcgacgtt gatcccgagc gccttcagat catgggccat
     2641 aagccgtgcg ccgagccgga ccagttcgcg cgccgtagct gagtcgtcgg tcgcagccag
     2701 ataggtcgat ccgggcggat atttgggcca gtgcggcggg ccgagccgct gaacccggcc
     2761 gccttcctgg tcgatcagaa tcggtgcgtt aggatcatcg atcgaggcgc ggaggtcggc
     2821 gaccaacgcc ttgacctgtt ccggcgtgtc cacgttgcga cggaacagga tgaagcccca
     2881 aggccggacg tcggcgaaga actcacgttc ctcgtccgtg agtacgtgac cggaacagcc
     2941 gtagatcgcg gcgcttgtca ccgtgtcagc ggaccagaca atcgccgccg gccgactgga
     3001 tcgccgaaca gaaggcctgc gcccgttcac tgctgaggcc gtagaaggtg gtgcgataga
     3061 cggtcgagcc gttcgaggcg gtgacttcct gaacgcgctt ctgcgagcca cgggcgaact
     3121 gtgggaagcg tgctgcggtc gcggcgaatt cccggtcggc gatcgcagtc gaggagaagg
     3181 cgccgatctg gacagccgat ccgccggttg cgggggcaga cgtcggcggc gtctgcgtcg
     3241 cctgtggcga aggtcgtacc acgggtgcgc tcggcgtcac gggacgcgca ggcgtaggac
     3301 gggtcggcgc aggacgcgtg ggggccagtt cgtcagaggc gactggaggc agcggctttg
     3361 gcgcaggacg cggcagggct tgctcaggcg gcgctgtata ggtcggagat gtaacggcag
     3421 acggtgtctc gttgcgataa acgtcgacgc ctgcctcctc gtcgaccggc tgggcctcaa
     3481 ctggcgcctg aactttcagc tcaccgacag gcgtacctac cgcaggcggc acatcggtcg
     3541 aggcgcgcag gccggagcgg tagaacagca ccaccgccac gatcagaacc agcagcacca
     3601 ccgcgctgac gatcagcgta atcgggggcg ccttggccgg cggtgaccga cgcgcatcga
     3661 aaccgccccg gttgaacggc aggtcgtcgt cggtcggcgg cgtgtacgga ccacggtcac
     3721 ggctgtcgtt cgggcggttg ttatcgtcat aggacatggg cgtatttctc tggaccggcg
     3781 cgaatcggca cgcggtcgtt cactctaacc gctggccgcc tcttttcgaa tcacagttcg
     3841 cgcccgaggt cgagcttgaa cagcttcctg tgctcctcga tggcgtacat gtcggtcatg
     3901 cccgcgatat aatcgcacac ggcgcgggcg cgcttgtgca gatcgctgga tgacgcgcga
     3961 tccgaccatt cgggcggcag cacctccggc tcggccatga acagctggaa catttcggcc
     4021 aggatgcgtc gcgcctgact gcgcgtgcgg ttgacgcggt agtggcgata catccgctcg
     4081 aacagaaaga ttctcagcgc ccccagatcg gccagcatcc cgtctgaaaa ctggatcagg
     4141 gtgcgtttcg ccatccgtac gtcttcgggc gtcacgatct tgtcagcctc cagtcggcgc
     4201 gcagtttcgg ccaggacgtc atcgaccatg acgccgatca tcctccgcac ggcctccagc
     4261 cgcaccatcc gttcatcgag ctcgggccag tcggcccgca cacctttcaa caccggcccg
     4321 atcagcggca cctcggccag atcggcaagg gtgaacagac ctgcctgcac cccgtcgtcg
     4381 acatcgtggt tgttgtaggc gatgtcgtcg gcgatagccg cagcctgcgc ctctagcgac
     4441 gcaaacgtgt tcagtctcag gtcccagccg ggattgtcgc tggtgcgggt atagtcggcg
     4501 atggcctgcc aagcgggctc gtccagcttg tgggtcaccg gaccgttgtg cttgaccacg
     4561 ccctcaaccg tctcccacga caggttgagg ccgtcgaact cgggatagcg ctcctccagc
     4621 ttcgtcacca cccggaaggt ctggacgttg tgatcgaagc cgccatacgg cttcatctgc
     4681 acctgcagct cgtcctcgcc cgcatggccg aacgggggat gcccaagatc gtgggcaagg
     4741 gcgatggttt cggccagatc ctgatccagc ccgagcgcat gcgccagcga gcgggcgatc
     4801 tgcgcgacct caagactgtg agtcaggcgg gtgcggtagt gatcgccttc atgcgccacg
     4861 aacacctgcg tcttgccctt caggcgacgg aaggcggtcg agtggatgat ccggtcacgg
     4921 tcgcgcgcaa aggcgttgcg ggtacggctc ggaggctccg gcacgatgcg cccgagcgtg
     4981 tccgcaggcg tctcggcata gggcgcaaaa ccgcagcttg cggcgaatgg actggacgaa
     5041 ggaggctgct gg
//
LOCUS       DASVTM010000295        15197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_345839,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000295 DASVTM010000000
VERSION     DASVTM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 15197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15197
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345839"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VF633_08040"
     CDS             complement(<1..230)
                     /locus_tag="VF633_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5990 family protein"
                     /protein_id="HEX8471046.1"
                     /translation="MAEIMLRLTIADPVPGVRYSLQDPKSVPVESVVASAAPLSFDVP
                     IKLSDDGRLTGPFVRREGPTRRFVHIAIGQGAG"
     gene            complement(235..945)
                     /locus_tag="VF633_08045"
     CDS             complement(235..945)
                     /locus_tag="VF633_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8471047.1"
                     /translation="MSSEPEPFDADQDLKDAPRVASRMRPKDASTLILFRDGTAGPEV
                     LMGRRAPGHVFMASKWVFPGGRVERSDFTAAATGELTDAAASLHSEVTARRARALALA
                     AVRETFEETGLMLARPAPVASVAGSWRDFRAVGALPDLSVLTYVARAITPPGRTRRFD
                     ARFFMADASALLAPEPTAGSGELDEIAWLPLAEARTLELPAITRMVLGEAAQRRAEPH
                     RVPPFVRFVRGSHVITRD"
     gene            complement(997..1563)
                     /locus_tag="VF633_08050"
     CDS             complement(997..1563)
                     /locus_tag="VF633_08050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX8471048.1"
                     /translation="MRLVGISGSLRAGSFNTSLLRASQALVPADVELVAGSIHGIPLY
                     DGDVERDEGSPPAVVALKELIAGADGLMLFTPEYNNGIPGVFKNAIDWLSRPSSDIAR
                     IFGGRPVAVLGASLSGFGTILSQAAWLPVLRTLGADHWAGERLMVARAGSVFDEDGKL
                     IDEAIRQRLEAFVTGFADHCRKTSAQNG"
     gene            complement(1566..3923)
                     /gene="rnr"
                     /locus_tag="VF633_08055"
     CDS             complement(1566..3923)
                     /gene="rnr"
                     /locus_tag="VF633_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX8471049.1"
                     /translation="MAKTPRSPSGPSTGGLPDKETLLRFLREAGEADKSDLARAFGLK
                     GGERRALREMLRELEAEGALGKRGKKGFSEAGALPPVGVVDVVERDLDGELYVRLVEA
                     AADAPRAILIPDKSGDSGPAPGMGDRLLVKFLRGTDGWEARVVKKLDNDGNRVLGVIR
                     KSARETRVEPVDRKSKDVLIIPQLLAEGLRDGDLVLAAIEKGVQRYGPKRGKILETIG
                     REDDPRAASLIAIHTHGVPTGFSEAVEKEAENQDLPTLKGRDDLRDIPFITIDPADAR
                     DHDDAVFAQKDDDEKNPGGWIVWVAIADVAAYVRPGAALDREARAKGNSTYFPDRVEP
                     MLPERLSNGLCSLKEGENRATLAVRMVFDKEGKKTGHKFHRGLMRSHAKLSYEQAQAA
                     IDGVENGGGTDDTTGPIMDAILYPLWNAYATLLKGRLKRSPLQIESAERRILMGKDGT
                     IAAIVPRKSLEAHRLIEEMMIQANVCAAETLEQKKTPLIYRVHEAPSQEKIFNLADFL
                     TTIAMPWNKGEPATTKRFNKLLDQTREGPHADVVNEVVLRSQMQAVYSPDNLGHFGLH
                     LDRYAHFTSPIRRYSDLIVHRGLIRALKLGSDGLTDREIAELQSIAEQVTLTERRSMA
                     AERAAMDRYIAAFLEDHVGAAFDGRITGVTRFGLFVRLEETGADGLVPVSSLGDEYFT
                     HDDRAHALVGERSGQRYTLGRTVQVKLKEATPVTGGLLFEMLSDAEPRDPNAPAPRYG
                     VRARDRGPGGSGPPKRGSGRPGGPKPPRGGRPAGGLKGVRKGKRK"
     gene            complement(3979..4497)
                     /locus_tag="VF633_08060"
     CDS             complement(3979..4497)
                     /locus_tag="VF633_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX8471050.1"
                     /translation="MASDQQSGDPISARNRAAEIHDFARSELFDRTFREGMELVEDTA
                     SYLDGDGRRESRLLSRAAALAYAAESMKLTTRLMQVASWLLVQRAVREGDMDPDAACE
                     PRYRLTMRPSESQPSHAELPIALIEYVVRAEKLHDRVLYLDRRMYLDVPAEAEVNPVL
                     SQHGMLEAAFRS"
     gene            complement(4630..4836)
                     /locus_tag="VF633_08065"
     CDS             complement(4630..4836)
                     /locus_tag="VF633_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX8471051.1"
                     /translation="MTFEDLEPRSGRGATLTALARDDLDLCSVEELNERIEALQAEID
                     RAKTAIDGKQAKKSAADALFNFQS"
     gene            4912..5883
                     /locus_tag="VF633_08070"
     CDS             4912..5883
                     /locus_tag="VF633_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX8471052.1"
                     /translation="MRTIEITGGAGPAEALKSAERPDPVAGPGQVRIRVRAAGVNRPD
                     LLQRSGRYPPPPGASDILGLEVAGEIDQVGKGVTRWNTGDRVCALLGGGGYAELAVVD
                     ARHVLPIPDGLDFVNAAVLPETVFTVFTNVFEGGSLKAGETLLIHGATSGIGVTAIQM
                     AKAAGARVIATSRGPEKTAAALALGADISLDAMTDDLAAALAEAGGVDVVLDMVGADY
                     AALNLKALKPFGRWVVIASLSGAKVEINLAELMMKRIVLTGSTLRARPADEKARLAAA
                     VEATVWPWVASGQIKPPIDATFPLEQAAEAHLRLEAGQHIGKVVLTV"
     gene            complement(5887..6777)
                     /locus_tag="VF633_08075"
     CDS             complement(5887..6777)
                     /locus_tag="VF633_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HEX8471053.1"
                     /translation="MSIKTQITVAATTLAALALLAPMSASAQRNGGGNGGVPGGSYAQ
                     SCSNASVQGGRLYAECRGIGRNSRPTSSSIEFGRCTGDIANSEGMLVCNGAQGRPESG
                     NGGGGNNGGGNNGGNGGRPAGSYAQTCSNVRVEGGRLYAECRDMSRQTRASSIEYGRC
                     TGGINNIDGLLTCDGTRGRFEGRGGNNGGGWGGGNGNGGGNNGGGWGGNNGGRNSITV
                     YEDSNYRGGSTTFNREMPNLGRRELNDNISSMEMNGSWEACSDAYFRGNCQTFTGSVR
                     NLNSTVLNDRISSLRPVRGW"
     gene            6999..7664
                     /locus_tag="VF633_08080"
     CDS             6999..7664
                     /locus_tag="VF633_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEX8471054.1"
                     /translation="MTDILMPKATAVWMVDNTSLTFEQIADFCGLHPLEVRGIADGDV
                     ARDIRGVDPITGGQLTREELDKAQDNAEYRMKAIVSRHAELIKAHKPAPKYTPVSRRQ
                     DRPDAISWFVRNHPEVTDAQIAKLLGTTKSTIESVRNRTHWNSPNIKPVDPVTLGLVG
                     QLVLDDLISKAAAKKNKDDLKKGGGVLQPVEAEPVEEQFVPDAVERRTAEPTAASVFG
                     NKN"
     gene            complement(7771..7944)
                     /locus_tag="VF633_08085"
     CDS             complement(7771..7944)
                     /locus_tag="VF633_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX8471055.1"
                     /translation="MTIEARIRELGNRHRQLDESIQREVGRPATDPLQVRELKRRKLR
                     LKEEITSLEARAG"
     gene            8070..8723
                     /locus_tag="VF633_08090"
     CDS             8070..8723
                     /locus_tag="VF633_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX8471056.1"
                     /translation="MDHDVNGAVRNPATSERPHSPTGARPRLVVGISGASGVIYGVRL
                     LDALRELDVESHLVVTRAALLTLSQETDLTPDDLTERADVTHRLNDVGASIASGSFKT
                     LGMIVAPCSVRTMSEIATGVTSSLLTRAADVVLKERRPLVLMVRETPLHLGHLRTMTS
                     LAEMGAIIAPPLPAFYSKPASIAEMVDQSVGRVLDLFGLDWSPVKRWDGLKGPAVEE"
     gene            8720..9190
                     /locus_tag="VF633_08095"
     CDS             8720..9190
                     /locus_tag="VF633_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HEX8471057.1"
                     /translation="MSERLWDWALAAYARPGVADACLNLQDGTGQNVPLLLWAAWSAT
                     TGRRPDEDEIEAACDTARAWADTAVTPLRTIRRALKSPIPDLDDGAREAVRDQIKAVE
                     LLAERKLLDGLEALAPVPSGAPRRAIEGLAEVARVWARVVPRPALLELAERLPA"
     gene            9246..9470
                     /locus_tag="VF633_08100"
     CDS             9246..9470
                     /locus_tag="VF633_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX8471058.1"
                     /translation="MNDDEPFLTDDEQALHAEVKLMRQEHSDLDAAIEALGHMPIPDQ
                     LRIARLKRKKLQLRDEIVKIESRILPDIIA"
     gene            9571..10680
                     /gene="tgt"
                     /locus_tag="VF633_08105"
     CDS             9571..10680
                     /gene="tgt"
                     /locus_tag="VF633_08105"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX8471059.1"
                     /translation="MPFPFDISATEGRARTGVLKTSRGDIRTPAFMPVGTVATVKAMT
                     VDQVKATGADIILGNTYHLMLRPGPERMERLGGLHKFMGWEKPILTDSGGFQVMSLAG
                     ISKVKEEAVTFSSHIDGSKHVLSPERSIEIQADRIGADISMQLDQCVAYPAEKDAARK
                     AMELSVRWGARSKAAFGTRETQGLFGIQQGSTFEDLRRQSSDQLRQIGFDGYAIGGLA
                     VGEGHQAMCEVLDYAPDMLPADRPRYLMGVGKPIDLVEAVYRGVDMFDCVLPTRAGRH
                     GQAWTWDGPMNLKNARFAEDQDPLDPTIDGPSSTYSKAYLHHLIRADEILGKVLLSWH
                     NIAFFQALTAAMRDAIQHGRFEQFRRDFHARHTSN"
     gene            10697..11005
                     /locus_tag="VF633_08110"
     CDS             10697..11005
                     /locus_tag="VF633_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471060.1"
                     /translation="MRIEKSGFHAYSVYLEEEPRPDGNETALHRHVIIIGGDKYSFFA
                     HWSGKFAHKGERISFDWDWDRTGEFKNIDKSSFEAFAKDGTVQIRGDRSDKRRPASGR
                     "
     gene            complement(11189..11725)
                     /locus_tag="VF633_08115"
     CDS             complement(11189..11725)
                     /locus_tag="VF633_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471061.1"
                     /translation="MRISVSSVALLCLALAGCSAAGQVQRGFGDAVTSPLVDLNVRRD
                     QIPTVLLQAQANPYDQRNLNQCSTVSAEIARLNEALGPDRDEPAAPNAATLNERAQDA
                     LAKAALDAIKNAATDFIPYRNWIRALTGAQARSKTVQDSIEAGRLRRAFLKGLGMRSN
                     CSPPASPAWFRPSGRSQR"
     gene            11914..12354
                     /locus_tag="VF633_08120"
     CDS             11914..12354
                     /locus_tag="VF633_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8471062.1"
                     /translation="MTLAGMFAAALISSGQIQTPPTAPAAPRPMVVVREADVQVEQSP
                     PPHAGVGRSTAFRLSDAAPGRNFEFRKRILHPGASIGLHVLTHDEVYYVVSGQGEVAS
                     DGRTETIGSETAAYLYEGADVGIRQTGTEPLVLIIAYPLAARTQ"
     gene            complement(12360..12887)
                     /locus_tag="VF633_08125"
     CDS             complement(12360..12887)
                     /locus_tag="VF633_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX8471063.1"
                     /translation="MTVYALGDKKPQLPPEGEYWIAPNAVVAGDVILKAGASIWFGCV
                     LRGDTDTLTVGENSNIQDLSVLHADAGSPLTIGRNVTVGHKVMLHGCTIGDNSLIGIG
                     AVILNGAVIGKNCLIGANALITENKIIPDGSMVVGQPGKVVKELSEGQIAALEMSAQH
                     YVQNWKRFAAELRPI"
     gene            13241..14029
                     /locus_tag="VF633_08130"
     CDS             13241..14029
                     /locus_tag="VF633_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX8471064.1"
                     /translation="MTKRAAMKRPNREGVFDTRQFSEDSKVRRLPTPHTTAAAKGGAW
                     APHPSNDDREQGYLKTLKPRSDGQAELLNAIDASNLCVALGPAGTGKTYLAVAKAVEA
                     LEAGKVGRIVLSRPAVEAGESIGFLPGAMEDKLAPYLRPLYDALSDRLTMKRVGAMMA
                     EGLIEIAPVGYMRGRTLNNAFIVIDEAQNCTYVQLKMLLTRLGWHSTMVVTGDPGQTD
                     LLPSLSGLSDIAQRLEAVPGISVVRLAEQDIVRHPLVASMIGVL"
     gene            complement(14088..>15197)
                     /locus_tag="VF633_08135"
     CDS             complement(14088..>15197)
                     /locus_tag="VF633_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8471065.1"
                     /translation="FVADTELAPQLAEIGVILLMFGVGLHFSPADLMKVRKVAIPGAL
                     FQIAAATGLGWALGRLLGMDNLEAALMGFALSVASTVVLLRALEERRQVKGDIGGIAV
                     GWLIVEDLVIVIALVMLPLLIVQPGQELSGAELAGSIGWTLAKVAVFVGAMLFIGPRV
                     LPWVLVRIAHTKSRELFTLGVLAIALGIAWIAYFMFHSFALGAFLAGLVLNSSPLGHN
                     AAERSLPLRDAFAVLFFVSVGMLFDPMILVNRPLTVLGVLGIVIVGKALAALVITFAF
                     KLDKATSLTIAASLAQIGEFSFILAALGVTLGALSQETHDLILAAALMSISLNPFVFK
                     LTDRLGGRPKAGTPAAKALEVEKAAEARKPNPATA"
BASE COUNT         2618 a         4873 c         5085 g         2621 t
ORIGIN      
        1 cctgcgcctt gaccgatagc gatgtggacg aaacgtcgtg ttggcccctc gcggcgcacg
       61 aacggtccgg tcaagcggcc gtcgtcggac agcttgatcg gaacgtcgaa agacagcggg
      121 gcggccgacg ccaccacaga ctcaaccgga acggatttcg gatcctgcaa actgtagcgc
      181 accccgggga caggatcggc gatggtcagg cgaagcatga tctcggccac ggcgtcagtc
      241 ccgggtgatg acgtgcgaac cgcgcacgaa acggacgaaa ggcgggaccc ggtggggttc
      301 ggcgcgtcgt tgtgcggcct cgcccagcac catgcgggtg atggccggga gctccagcgt
      361 ccgcgcttcc gccaacggga gccacgcgat ctcgtcgagc tctcctgatc cggcggtggg
      421 ttcgggggcc agcagggccg aggcgtcggc catgaagaac cgcgcgtcaa accggcgggt
      481 gcgccccggg ggcgtgatgg cgcgcgcgac ataggtcagc accgacaggt cgggcagggc
      541 gcccacggcc cggaagtcgc gccacgatcc ggccaccgag gctacgggcg cgggacgggc
      601 caacatcagg ccggtctcct cgaaggtctc ccgcacggcg gcgagggcca gagcgcgggc
      661 gcgtcgggcg gtaacttcgg aatgaaggct ggcggcggcg tccgtaagtt cgccagtcgc
      721 tgcggcggtg aagtccgaac gttcaacacg tccgccgggg aagacccatt tggaagccat
      781 gaagacgtgc ccgggggcgc ggcggcccat caggacctcg ggacccgcgg tcccgtcgcg
      841 gaagaggatc agggtggagg cgtccttcgg gcgcatccgg cttgcgacgc gcggagcatc
      901 cttcaggtcc tggtcggcgt caaaaggttc gggctcggac gacatggcgt catgcgatgc
      961 cacgcggtcg gggcgaaggg aaggcagctt gccgcgtcag ccgttctgag cggatgtctt
     1021 gcggcaatga tccgcgaagc cggtgacgaa ggcctctaga cgctgtcgga tcgcctcgtc
     1081 gatcaacttg ccgtcctcgt cgaacaccga accggcgcgg gcgaccatca ggcgttctcc
     1141 ggcccagtga tcggctccga gggtgcgcag gacgggcagc caagccgcct ggctcaggat
     1201 ggtcccgaac ccgctcagag acgcccccag caccgctact ggccgcccgc cgaagatgcg
     1261 ggcgatgtcc gacgaggggc ggctgagcca gtcgatggcg ttcttgaaca cgccgggaat
     1321 gccgttgttg tattccgggg tgaacaacat cagcccgtcc gccccggcga tcagctcctt
     1381 gagcgccacg acggcgggcg gcgaaccttc gtcgcgctcc acatcgccgt cgtacaacgg
     1441 gatgccgtgg atggagccgg cgaccagttc gacgtcggcg gggacaagcg cctgcgacgc
     1501 acgcagcagg gacgtgttga aagatcccgc tcgcaggctg cctgagatgc cgaccagtcg
     1561 cacagtcact tccgcttgcc cttgcgcacg ccttttagcc cgccggccgg ccgccctccc
     1621 cggggaggct tgggaccgcc gggacggccg gagccgcgct tgggtgggcc gctgccgccg
     1681 gggccacggt cgcgagcgcg gacgccgtag cggggggcgg gggcgttggg gtcgcggggt
     1741 tcggcgtcgg acagcatctc gaacaacagg ccgccggtga cgggcgtggc ttccttgagc
     1801 ttcacctgaa cggtgcgacc cagggtgtag cgctgacccg agcgttcgcc gacgagggcg
     1861 tgggcgcggt cgtcgtgggt gaaatactcg tcgcccagcg aggagacggg cacgagaccg
     1921 tcggcccccg tttcttccag ccgtacgaac aggccgaagc gggtcacgcc ggtgatccgg
     1981 ccgtcgaacg cagcgccgac gtgatcctcg aggaaggcgg cgatgtagcg gtccatggcg
     2041 gcgcgctcgg cggccatgga gcgacgctcg gtcagggtca cctgttcggc gatggactgg
     2101 agttcggcga tctcacggtc cgtcaggcca tccgacccca gcttcagcgc ccggatcagg
     2161 ccccggtgga cgatcaggtc ggaatagcgc cggatcggtg aggtgaagtg ggcgtagcgg
     2221 tcgaggtgca gaccgaagtg gccgaggttg tcaggcgagt agaccgcctg catctggctg
     2281 cgcagtacga cctcgttgac gacgtcggcg tgcgggcctt cgcgggtctg gtccagcagc
     2341 ttgttgaacc gcttggtcgt cgccggctca cccttgttcc acggcatggc gatggtggtc
     2401 aggaagtcgg ccagattgaa gatcttctcc tgactgggcg cctcgtggac gcgatagatc
     2461 aggggtgtct tcttctgctc cagcgtctcg gcggcgcaga cgttggcctg gatcatcatc
     2521 tcctcgatca gccggtgggc ctcgagggat ttacgcggga cgatggcggc gatggtgccg
     2581 tccttgccca tcagaatgcg acgttcggcg ctctcgatct gcagcgggct gcgcttgagg
     2641 cggcccttca gcagcgtcgc ataggcgttc cacagcggat aaaggatcgc atccatgatc
     2701 gggccggtgg tgtcgtcggt gccgccgccg ttctcgacac cgtcgatagc agcctgcgcc
     2761 tgttcgtagc tgagcttggc gtgcgaccgc atcaggccgc ggtggaattt gtgcccggtc
     2821 ttcttgcctt ccttgtcgaa gaccatgcgc acggccagcg tggcgcggtt ctcgccttcc
     2881 ttgaggctgc acaggccgtt cgacaggcgt tcgggcagca tcggctcgac gcgatcggga
     2941 aaataggtcg agttgccctt ggcgcgggcc tcgcggtcca gcgcggcgcc cgggcggacg
     3001 taggcggcga cgtcggcgat ggcgacccag acgatccagc cgccggggtt cttctcgtca
     3061 tcgtccttct gcgcgaagac ggcgtcgtcg tggtcgcgag catcagccgg gtcgattgtg
     3121 atgaagggga tgtcgcgcag gtcgtcgcgc cccttgagcg taggcaggtc ctgattttcg
     3181 gcctccttct cgacggcttc ggagaagcct gtcgggacgc cgtgggtgtg gatggcgatc
     3241 agggaggcgg cgcggggatc gtcctcgcgt ccgatagtct caagaatttt gccgcgtttg
     3301 ggcccgtagc gctgaacgcc tttctcgatg gcggccagca ccagatcgcc gtcgcgcaga
     3361 ccctcggcca agagctgcgg gatgatcagc acgtccttgg acttgcgatc gaccggctcg
     3421 acccgggttt cgcgggcgga tttgcggatc acgccgagga cgcgattacc gtcgttatcc
     3481 agcttcttga cgacgcgggc ttcccagccg tccgttccac gcaggaattt gaccagcagc
     3541 cgatcgccca tgcccggggc cgggccgctg tcgccggact tgtcggggat caggatggcg
     3601 cgaggcgcgt cggcggcggc ctcgaccagc cggacgtaca actcgccgtc gagatcgcgc
     3661 tcgaccacgt cgacgacgcc gacggggggc agggcgccgg cttcggagaa gcccttcttg
     3721 ccacgcttgc ccagcgcacc ttcggcttcg agctcgcgca gcatttcacg cagcgcgcgt
     3781 cgttcaccgc ccttcagacc gaaagcgcgg gcgagatcgg atttgtcggc ttctcctgcc
     3841 tcacgcagaa agcgcagcag ggtttccttg tcgggaaggc ccccggttga tggaccggac
     3901 gggctgcggg gagttttggc catgagtaga gattagcgcg gattttcagc acgcaccacg
     3961 aaactcacgg ggtcgaggtc aagatctgaa ggcggcttcc agcatgccgt gctggctaag
     4021 caccggattg acctcggctt cggcgggaac atcgagatac attcggcggt ccagatagag
     4081 gacgcggtcg tgcagctttt cggcccggac gacatattcg atcagggcga tgggcagttc
     4141 ggcgtggctg ggctggctct cgctcggtcg cattgtcagc cgatagcgcg gttcgcaggc
     4201 ggcgtcggga tccatgtccc cttcgcgaac tgcgcgctgg accaatagcc aggacgcgac
     4261 ctgcatcagc cgggtcgtca gcttcatgct ctcggcggca taggccaggg cggcggcgcg
     4321 tgacagcagc cgggactcgc gacggccgtc tccgtccaga taggatgccg tgtcttcgac
     4381 cagttccatg ccttcgcgga aggtgcggtc gaaaagctcc gaccgcgcga agtcgtggat
     4441 ctccgcagcg cggttgcgcg cggaaatcgg gtcgccggac tgttggtcgc tggccataga
     4501 ctcaatcata ctcatggaag gtgcgcgcgt ccgtctcgtt ctcgccccgc aagggcgccg
     4561 tcaagacaag gtctgcaacc gccatgccat gaaccgggga tacggaaaac aattcgggga
     4621 cgaaacaggt cacgattgaa agttgaacag ggcgtctgct gcgcttttct tggcctgttt
     4681 gccgtcgatg gcggttttcg ctcgatcgat ttcagcctga agagcctcga tccgctcgtt
     4741 cagttcctcg accgaacaga gatcgagatc gtcgcgggcg agcgccgtca gggtggctcc
     4801 gcgtcccgag cgcggctcca gatcttcgaa agtcatcgtc acctccatgg ccatgccccg
     4861 cccaggaccc tatctgaacg ccgtgagagc tatgatgcaa ggtttgaccg gatgcggacg
     4921 attgagatca ccggcggcgc aggccctgcc gaagccctga aaagcgccga acggcccgat
     4981 cccgtagcgg gacccggaca ggttcgcatt cgcgttcggg ccgccggggt taaccgacca
     5041 gacctgctgc agcgctccgg ccggtatccg cccccccctg gagcctcgga catcctcggc
     5101 ttggaagtgg caggcgagat cgatcaggtc gggaaaggcg tgacccgatg gaataccggc
     5161 gatcgcgtct gtgcgctgct gggcggcggt ggttatgcgg aactggcggt ggtcgacgcc
     5221 cggcacgttc tgccgatccc ggacggactg gacttcgtca acgccgcggt gttgcccgag
     5281 accgtcttca ccgtcttcac caatgtcttc gaaggcgggt cgctgaaagc gggcgagacc
     5341 ctgctgatcc acggcgcgac cagcggcatc ggcgtcacgg cgatccagat ggccaaggca
     5401 gcgggggccc gggtcatcgc aacatcgcgt ggcccggaga aaacggcggc ggcgcttgct
     5461 ctcggggccg acatcagcct cgacgccatg accgacgatc tggcggcggc gctggccgag
     5521 gcaggcgggg tcgatgtagt gctggacatg gtcggcgcgg attatgccgc gctgaacctg
     5581 aaggcgctga agcctttcgg gcgctgggtc gtgatcgcgt cgctgtccgg ggcgaaggtc
     5641 gagatcaatc tggcggaatt gatgatgaag cgcatcgtcc tgaccggatc gacgcttcga
     5701 gcccggccag ccgacgaaaa agccaggttg gcggcggcgg tcgaggcgac ggtctggccg
     5761 tgggtggctt cgggtcagat caagccgccg atcgacgcga ccttcccgct ggagcaggcc
     5821 gctgaggccc acctccggct ggaggcgggc caacatatcg gcaaggtcgt cctgacggtt
     5881 tgaggcttac cagccgcgga ccggacgcag cgacgagatg cggtcgttga ggacggtgga
     5941 gttcaggtta cggacgctgc cggtgaaggt ctggcagttg ccccggaaat acgcgtctga
     6001 acaggcttcc caggaaccgt tcatctccat cgaagagatg ttgtcgttca gctcgcgccg
     6061 gccgaggttg ggcatctcgc ggttgaaggt cgtcgacccg ccccggtagt tcgagtcctc
     6121 atagacagtg atcgagttgc ggccgccgtt gttgccgccc caaccgccgc cgttgttgcc
     6181 gccgccgttt ccgtttccgc cgccccagcc tccgccgttg ttgcctcccc ggccttcaaa
     6241 ccgcccacgc gtgccgtcgc aggtcagcag gccatcgatg ttgttgatcc cgccggtgca
     6301 gcgaccgtac tcgatcgagg acgcccgggt ctgtcggctc atgtcccggc actcggcgta
     6361 gaggcggccg ccttcgacgc gaacgttcga gcaggtttgg gcgtagctgc cggcgggccg
     6421 cccgccgttg ccgccattgt tcccgcctcc gttgttgcct ccgccgccgt taccgctttc
     6481 cgggcggccc tgcgcgccgt tgcagaccag catcccctcg ctgttcgcaa tatcgcctgt
     6541 gcagcgaccg aactcgatcg agcttgaagt tggccgggag ttgcggccga tgccgcgaca
     6601 ttccgcgtaa agccggcctc cctggaccga agcattggag cagctctggg cgtagctccc
     6661 ccccgggacg ccgccgtttc cgccgccgtt ccgctgggcg ctggccgaca tgggcgcgag
     6721 cagggcaagc gccgccaagg tcgttgccgc gacggtgatt tgggtcttga tcgacatggt
     6781 gttccctcca gaacggtagt gcggctcggc cgcgttatcg ccactctcga ttactgaagc
     6841 tgaaccgtag ctgtacgcca gtgacagttc ctgtcactca gcgttttctt ttgtgacgaa
     6901 acagcctata tcccgaccat cgcgcccggc cgaaaggtcg ggcgccgttg tatccagaac
     6961 ccgtcaaacc gggtcgaaac cttccgggaa ttacgcccat gaccgacatc ctcatgccca
     7021 aggccactgc ggtctggatg gtggacaata cctccctgac cttcgagcag attgccgatt
     7081 tctgcggtct gcaccccctt gaagtgcgcg gcattgccga cggcgacgtg gcgcgcgaca
     7141 tccgtggcgt cgatccgatc accggcggtc agctgacgcg cgaagagctc gacaaggccc
     7201 aggacaacgc cgaataccgc atgaaggcca tcgtcagccg tcatgccgaa ctgatcaagg
     7261 cgcacaagcc agccccgaaa tacacgccgg tctcgcgccg tcaggaccgt ccggacgcca
     7321 tctcgtggtt cgtgcgcaac caccccgaag tgaccgacgc ccagatcgcc aagctgttgg
     7381 gcaccaccaa gtcgaccatc gaaagcgtgc gtaaccgcac ccactggaac agccccaaca
     7441 tcaagccggt cgatccggtg accctcggtc tggtgggcca actggtgctc gacgatctga
     7501 tctcgaaggc tgcggccaag aagaacaagg acgatctcaa gaagggcggc ggcgtgctgc
     7561 agcccgtcga ggccgagcca gtcgaggagc agttcgttcc cgacgccgtc gagcgccgca
     7621 ctgcggaacc gaccgcagcc tcggtgtttg gcaacaagaa ctaagagcca ctccctctcc
     7681 ctctcccaac gggacagggc gactaaaaaa aggccccggg atcgctcccg gggcctttcc
     7741 aattcgcttt ctattctggg tcggcccgag ctatcccgcc cgggcctcaa gactggtaat
     7801 ctcttccttc agccggagct tccttcgctt gagctccctg acctgcagcg gatccgtggc
     7861 cggtcggcca acctcccgct ggatggattc atccagctgg cggtgacgat ttcccagttc
     7921 gcggatacga gcttcgatgg tcatggcttg cgcctccatg tctaagggac cacacagtga
     7981 gcgcccggat cggcggcctg tgaaatcacg gttgagtgac gtcaacagac ggttaaaccg
     8041 ctccgaaccc ggagagcgtg acccccaata tggaccatga tgtgaacgga gccgtacgga
     8101 accctgcgac ctctgagcgc cctcactctc caactggggc ccgtcctcgg ctggtggtgg
     8161 gaatctccgg cgcctcgggt gttatttatg gtgttcgcct gctcgacgcg ctgcgtgaac
     8221 tggatgtcga gagccatctg gtggtgacgc gggcggccct gctgaccctg tcgcaggaga
     8281 ccgatctgac gcccgatgat ctgaccgaac gcgccgatgt gacgcaccgc ctgaatgacg
     8341 tgggggcttc gattgcctcg gggtcgttca agacgctggg catgatcgtc gcgccctgtt
     8401 cggtgcggac catgagcgag atcgcaacgg gagtgacgtc gtcgctgctg acccgcgccg
     8461 ccgacgtggt gctgaaggaa cggcggccgc tggtgttgat ggtgcgcgag acaccgctgc
     8521 atctgggcca tctgcggacc atgacgtcgc tggccgagat gggggcgatc atcgccccgc
     8581 cgctgccggc cttctattcc aaaccggcgt cgatcgccga gatggtcgat caatcggtcg
     8641 gtcgggtgct ggacctgttc ggcctcgact ggtcgcctgt gaagcgctgg gatgggctga
     8701 agggcccggc ggtcgaagaa tgagcgagcg gctgtgggat tgggcgctgg cggcctacgc
     8761 cagaccgggc gtggccgatg cctgcctgaa cctgcaggac gggacgggcc agaacgtgcc
     8821 gctcctgctg tgggcggcgt ggagcgcgac gaccggtcgg cgacctgacg aggacgagat
     8881 cgaagccgcc tgcgacaccg ctcgagcgtg ggccgacacc gccgttacac ctctgaggac
     8941 catccgccgg gctctgaaat cacccatccc cgatctggac gatggcgcgc gcgaggccgt
     9001 gcgcgatcag atcaaggcgg tcgagctgct ggccgagcgc aagctgctgg acgggctgga
     9061 agccttggcc ccggtaccgt cgggtgctcc gcgtcgcgcc atcgaggggc tggccgaggt
     9121 tgcccgcgtc tgggcccgcg tggtcccgcg tcctgcgttg ctggaactcg ctgaacgcct
     9181 tccggcctga gcctcggtcg gctaagaagg cgacgcttcc ctgtgcgggg agtcggggct
     9241 gattcatgaa cgacgacgaa cccttcctga ccgacgacga gcaggctctg cacgccgagg
     9301 tcaagctgat gcggcaggag cattccgacc tcgacgccgc gatcgaggcg ctgggtcata
     9361 tgccgatccc cgaccagctc cgtatcgccc ggctgaagcg caagaagctg caactgcgcg
     9421 acgagatcgt gaagatcgaa agccgcatcc tgcccgatat catcgcctga ccggctgagg
     9481 ggcctaggtc ggtcggggtc gctttttcac agtttcgccc cacgcgatcc tgtatgacgc
     9541 gcgatcattg aagacgcgcc ggatcccgcc ttgccctttc cgttcgacat ttccgccacc
     9601 gaaggccgcg cccgcaccgg ggtgttgaaa acctcgcgcg gcgacatccg cacccccgcc
     9661 ttcatgccgg tcggcaccgt cgccacggtc aaggcgatga cggtcgatca ggtcaaggcg
     9721 acgggcgccg acatcatcct cggcaacacc taccacctga tgctgcgccc cggtccggag
     9781 cggatggagc ggctgggcgg gctgcacaaa ttcatgggct gggaaaaacc catcctgacc
     9841 gactcgggcg gctttcaggt catgtcgctg gcaggcatct ccaaggtgaa ggaggaggcg
     9901 gtgaccttct ccagccacat tgacggctcg aaacacgtcc tgtcgcctga gcgttcgatc
     9961 gagattcagg ccgaccgcat cggcgccgat atctcgatgc agctcgacca gtgcgtcgcc
    10021 taccccgccg agaaggatgc agcgcgcaag gcgatggagc tgtcggtgcg ttggggcgcg
    10081 cgctcgaagg cggcgttcgg gacgagggag acccaaggcc ttttcggcat ccagcagggc
    10141 tcgaccttcg aggatctgcg ccgccagtca tccgaccagc tgcgccagat cggctttgac
    10201 ggctacgcca tcggcggtct ggccgtgggc gaggggcatc aggcgatgtg cgaggtgctc
    10261 gactatgcgc cggacatgct gcctgccgac cggccgcgtt atctgatggg cgtcggcaag
    10321 ccgatcgatc tggtcgaggc ggtgtatcgc ggcgtcgaca tgttcgactg cgtcctgccc
    10381 acccgcgccg ggcgtcacgg tcaggcctgg acctgggatg ggccgatgaa cctcaagaac
    10441 gcgcggttcg ccgaggatca ggatccgctc gacccgacca tcgacgggcc gtcatccacc
    10501 tattccaagg cctatctgca ccacctgatc cgggccgatg agatcctcgg caaggtgctg
    10561 ctgagctggc acaacatcgc cttcttccag gccctgaccg ccgcgatgcg tgatgcgatc
    10621 cagcacggcc gcttcgaaca gttccgccgc gatttccacg cccggcacac gtcgaactag
    10681 tcgcaggagc cattccatga ggatcgagaa gtcaggcttc cacgcttaca gcgtctatct
    10741 ggaggaagag cctcgcccag acgggaacga gaccgcgctc caccggcacg tcatcatcat
    10801 cggtggcgac aagtactcgt tcttcgccca ctggtccggc aaattcgcac acaagggcga
    10861 gcgcatttcc ttcgactggg actgggacag gaccggcgag ttcaagaata tcgacaagtc
    10921 gtcgttcgag gccttcgcca aggacggaac cgtccagatc agaggcgacc gcagtgacaa
    10981 gcgccgcccc gccagcggtc gttaaatccc gtcggtgaac ccgaggatca tgccgtctat
    11041 gaagcccccg atcgcgtcta ccaagagagc tcgcatcgcg aaagccacga gcaggatgat
    11101 cccgatctac tggaaacggg atggcttggc gggagacatg gttcaactcc aggatgcagc
    11161 atcacgttcg ccgcaacgga gctggctgtc aacgctgcga gcgccccgag ggccggaacc
    11221 aggcgggcga ggccgggggc gaacagttgg accgcatccc cagacccttc aggaaggcgc
    11281 gacgcaggcg gccagcttcg attgaatcct gaaccgtttt cgagcgggcc tgtgctccag
    11341 tcagggcacg gatccagttg cggtaaggga tgaagtcggt ggcggcgttc ttgatggcgt
    11401 cgagggcggc cttggccaag gcgtcttgcg cgcgttcgtt cagcgtggcg gcgttggggg
    11461 ccgccggctc gtcgcggtcg gggccgagcg cctcgttaag ccgggcgatc tctgcgctga
    11521 cggtggagca ctggttcagg ttgcgctggt cgtagggatt ggcctgggcc tggagcagga
    11581 ccgtcgggat ctggtcgcgg cgcacattca gatcgaccag aggcgaggtg accgcatcgc
    11641 caaagccgcg ctggacctga cccgccgccg aacagccggc cagagcgaga cacagaagag
    11701 cgaccgacga gaccgaaata cgcatgccgc cattcaccac ggcagggcgg catcgacaac
    11761 acgtccatac atcgtgcttt cgtggctcag accacgagag ggcagatcag cgttgccagg
    11821 cgagatcgcc cacgaatggg agagacttcc ggtcgcagtg tccgacggcg ccatccgggc
    11881 taggctttgc ctcccacctc tggagacttg gccatgacac tcgccggaat gttcgccgcc
    11941 gccctgatca gcagcggtca gatccagacg ccaccgacgg ctcctgccgc tccgcgaccg
    12001 atggtggtgg tgcgtgaggc ggatgttcag gtcgagcagt cgccgccgcc ccatgccggg
    12061 gtcgggcgca gcaccgcctt ccgcctgtca gacgccgcgc ccggtcggaa cttcgagttt
    12121 cgcaagcgta tcctgcatcc cggcgcgtct atcggcctgc acgtcctgac gcacgacgag
    12181 gtctattatg tcgtcagcgg gcagggcgag gtcgcctcgg acggacgcac tgagacgatc
    12241 ggctccgaga ctgccgccta tctgtatgaa ggcgcggatg tcggcattcg ccagaccggg
    12301 acggagccgt tggtcctgat cattgcctat ccgttggctg cccgaacgca gtaagccggt
    12361 tagatcggcc ggagctccgc cgcgaaccgc ttccagttct ggacatagtg ctgcgccgac
    12421 atctccagcg cggcgatctg gccctcgctg agttccttca cgaccttgcc gggttggccc
    12481 acgaccatcg agccgtcagg aatgatcttg ttctcggtga tcagggcgtt ggcgccgatc
    12541 aggcagttct tcccgattac cgcgccgttc aggatcacgg cgccgatgcc gatcaggctg
    12601 ttgtcgccga tggtgcagcc atgcagcatg accttgtgcc ccacggtgac attccgcccg
    12661 atcgtcaggg gcgaacccgc atcggcgtga agcacgctga ggtcctgaat gttgctgttt
    12721 tcacccactg tcagggtatc cgtgtcgcca cgcagcacac aaccgaacca gatgctggcg
    12781 ccggctttga gaatcacgtc ccctgcaacc acagcattgg gcgctatcca gtattccccc
    12841 tcgggcggaa gctgcggttt cttgtctccg agtgcataca cagtcatcac aacaccgtca
    12901 catctaaagg aaatgagcgc tttaagctct cgtaaaccac gttagcatca gagtgttgat
    12961 gcgattcgag gtcgaccttc gaccccggat ccgcaaccgg atcaagcccg gtccggtcat
    13021 agcccaggag gacgtgagtg acgcgttcag ttcggcggcg acccatgcct cacgaggttg
    13081 gctcgccgga gattggcctc tcatccgacc ctggacggcg tatcgccgct tccctgcttt
    13141 ctatcttcgt tcgaggcgat gcttttgcgg catcgccttt tttcatgccc cgcgtcgatc
    13201 ggagcccccg gtcggcgtct ccccaaggaa tgaggcaacc atgaccaagc gtgcagccat
    13261 gaagcgccct aatcgtgaag gcgtgttcga tacccgccag ttctcggagg actcgaaagt
    13321 ccgccggttg ccgacacctc acacgacggc ggctgcaaag gggggcgcct gggcgccgca
    13381 tccatcgaac gacgaccgcg agcagggcta tctgaagacg ctgaagcctc gatccgacgg
    13441 tcaggccgaa ctgctgaacg ctatcgacgc atccaacctg tgcgtggcgc tcggcccggc
    13501 agggaccggc aagacctatc tggccgtggc caaggcggtc gaagcgctgg aagccggcaa
    13561 ggtcggccgc atcgtgctca gccgtcccgc cgtcgaggcc ggggagtcga ttggcttcct
    13621 gcccggcgcc atggaggaca agctggctcc gtatctgcgg ccgctctacg acgccttgtc
    13681 ggaccggctg acgatgaagc gggttggggc catgatggcc gaaggcctga tcgagatcgc
    13741 ccccgtcggc tatatgcgcg gtcgtacgct gaacaacgcc ttcatcgtca tcgacgaggc
    13801 ccagaactgc acctacgtgc agctcaagat gctgctgacg cggctgggct ggcattcgac
    13861 gatggtggtc accggcgacc cgggacagac cgacctgctg ccgtcgctgt cgggtctctc
    13921 ggacatcgcg cagcgtctgg aagccgttcc ggggatttcc gtggtgcgtc tggccgagca
    13981 agacatagtc cgtcacccgc tggtcgcgag catgatcggg gtgctctgac tcaaatataa
    14041 caatgagacc gaagggctgc cggagcgatc cggcagccct tttttaatca ggccgtcgcc
    14101 gggttcggct tcctggcttc ggcggctttt tcgacttcga gagccttggc ggcgggggtg
    14161 ccggccttgg ggcgtccgcc aaggcggtcc gtcagcttga acacgaacgg gttgagcgag
    14221 atcgacatca gggcggcggc gaggatcagg tcgtgcgtct cctgactgag cgcgccgagc
    14281 gtgacgccga gcgcggcgag gatgaagctg aactcaccga tctgggccag actggcagcg
    14341 atcgtcaggc tggtggcctt gtccagttta aacgcgaagg tgatgacgag cgcggcgagc
    14401 gccttgccga cgatgacgat gccgagcacg ccgaggaccg tcagcggccg attcaccagg
    14461 atcatcgggt cgaacagcat gccgaccgag acgaagaaca gcaccgcgaa cgcgtcgcgc
    14521 aggggcaggg accgctctgc cgcattgtga ccgagcggcg atgagttcag caccagcccg
    14581 gcgaggaacg cccccagcgc gaacgagtgg aacatgaaat aggcgatcca ggcgatgccc
    14641 agcgcaatag ccagaacgcc cagggtgaac agctcgcgcg acttggtgtg cgctatgcgg
    14701 accagcaccc agggcaggac ccgggggccg atgaacagca tggcgccgac gaagaccgcg
    14761 accttggcga gggtccagcc gatcgagccg gccagttctg cgccgcttag ttcctgaccc
    14821 ggctgaacga tcagcagggg cagcatgacc agggcgatga cgatcaccag atcctcgacg
    14881 atcagccagc cgacggcgat gccgccgatg tcgcccttaa cctgacgccg ttcctccagc
    14941 gcgcgcagca acaccacggt cgaggcgacc gacagggcga agcccatcag cgcggcttcg
    15001 agattgtcca tgccgagcaa tcgtcccaga gcccagccga gccccgtcgc ggctgcgatc
    15061 tggaacaggg ccccgggaat ggcgaccttg cgcaccttca tcaggtcggc cggagagaag
    15121 tgcaggccga cgccgaacat cagcaggatg acgccaatct cggccagttg aggcgccagt
    15181 tcggtgtcgg cgacgaa
//
LOCUS       DASVTM010000296         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_509432,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000296 DASVTM010000000
VERSION     DASVTM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509432"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2070)
                     /locus_tag="VF633_08140"
     CDS             complement(<1..2070)
                     /locus_tag="VF633_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HEX8471066.1"
                     /translation="MKLVHGYFPPWADRTPGAPVADMIPSAGFVPGIPRLGIPALRES
                     DASLGVANQVEQRKGDVATALPSSLATAASFDPDIAHAGGVMIGSEARSKTFNVLLAG
                     GVNLTRDPWSGRNFEYLGEDPLLAGTLAGAHIQGVQSNGIVSTIKHFAFNSQETGRMI
                     MDARLDEAALRESDLLAFQIAIERGRPGSVMCAYNRINGDYACENDFLLNQVLKRDWN
                     YPGWVMSDWGAVHSTEKAAVNGLDQESGQELDSRIFFGAPLAEAVAAGRVSEARLDDM
                     VTRILVGMIDTGAIDQSVPTTAQTPDYAAHAEVAQRAAEAGIVLLKNENGVLPLVATA
                     KRIVLIGGHADVGVLSGGGSSQVRSVGGAPVEIPLTSGAASSFARVTWHASSPLNAIK
                     GVAPDAEVVFVTGDDHAAAARAARGADIVVVFATQWTTEAQDVESLSLPDNQDALIAA
                     VAAANPHTAVVLETGGPVLMPWIDVVPAVVQAWYPGQAGGEAIARILFGQVNPSGRLP
                     ITFPRSADQPPRPAVPGLEAVKAHDEASNAGSGAAAAFAPFPVEYPEGSDVGYRWYER
                     EERAPLFPFGHGLSYTRFQYGALRIEGGASLTVSFDVTNAGDREGTDTPQLYVASEAP
                     GNARRLAGWARLTLKPGETRRVTITAEPRILAEYDTAAPGWKIAAGAYRVRIGHDALD
                     DELGGEAT"
BASE COUNT          344 a          726 c          752 g          350 t
ORIGIN      
        1 tgtcgcctcc ccgccgagtt cgtcgtcgag cgcgtcgtgt ccgatgcgca cgcgataggc
       61 tcccgccgcg atcttccagc cgggcgcggc cgtatcgtat tcggccagta tccggggttc
      121 cgcggtgatc gtgacgcgac gggtttcgcc cggcttcagc gtcaaccgcg cccagccggc
      181 caggcggcgc gcgttgccgg gagcttcgga agccacatag agctggggcg tgtccgttcc
      241 ctcgcggtcg ccggcgtttg taacgtcgaa gctgaccgtc aagctcgcgc caccctcgat
      301 gcggagagcg ccgtactgaa accgggtgta gctgaggccg tgcccgaacg ggaacagggg
      361 cgcgcgctct tcgcgctcgt accagcggta accgacgtcc gagccctccg ggtactctac
      421 cgggaagggt gcgaaggccg ctgcggcgcc ggagccggcg ttggacgcct cgtcatgggc
      481 cttcaccgct tccaggcccg gcacggccgg acgtggcggc tggtcagcgc tgcgggggaa
      541 ggtgatcggc aggcggccgg acgggttgac ttggccgaac aggatgcggg cgatggcctc
      601 gccgccggcc tggcccggat accaggcctg aaccacggcc gggacgacgt cgatccaggg
      661 catcagcacc ggtccgccgg tctccagcac cacggctgtg tgtggattgg cggcggcgac
      721 ggcagcgatc agggcatcct gattgtccgg cagggacagg ctttcgacgt cctgggcctc
      781 ggtcgtccat tgcgtggcga agaccacgac gatgtcagcc ccacgcgcgg cccgggcggc
      841 ggcggcgtga tcgtctccgg tcacgaagac gacctcggca tccggcgcga cgcccttgat
      901 ggcgttcagt ggcgaggagg catgccaggt cacccgggcg aaggacgagg ccgcgcccga
      961 ggtcagtgga atctccaccg gcgcgccgcc gactgaccga acctgggacg aacccccgcc
     1021 ggacagcacg ccgacgtctg cgtggccgcc gatgaggacg atacgtttcg ccgtcgcgac
     1081 cagaggcagc acgccgtttt cgttcttgag caggacgatc ccggcctcgg cggctcgttg
     1141 ggcgacctcg gcatgggccg cgtagtccgg cgtctgtgca gtcgtcggca ccgactggtc
     1201 tatggcgccg gtgtcgatca taccgaccag aatgcgcgtg accatatcgt cgagacgcgc
     1261 ctccgacacc cgcccggcgg ccacagcttc ggccaggggc gcgccgaaga atatccggct
     1321 gtccaactcc tgacccgact cctggtccag cccgttcaca gcagccttct cggtcgaatg
     1381 gaccgcgccc cagtcggaca tgacccaacc cggataattc cagtcgcgct tgagcacttg
     1441 attgagcagg aagtcgttct cgcaggcgta atcgccgttg atgcggttgt aggcgcacat
     1501 aacggatccc ggccgaccgc gctcgatagc aatctggaag gccagcaggt cgctttcgcg
     1561 caacgccgcc tcgtcgaggc gagcgtccat gatcatccgg ccggtttcct ggctgttgaa
     1621 cgcaaaatgc ttgatcgtgg agacgatacc gttcgactgg acaccctgga tgtgcgcccc
     1681 ggccaaggtc cccgccagga gtggatcctc gcccagatat tcgaagttgc ggccgctcca
     1741 ggggtcacga gtcaggttga ccccgccggc cagcaacacg ttgaatgtct tggatcgggc
     1801 ttccgagccg atcatgacgc cgcccgcgtg cgcgatgtcg ggatcgaagc tggcggcggt
     1861 ggccaaactg gatggcaggg ccgtcgctac gtcgcccttg cgctgctcga cctggttggc
     1921 cacgcccagg ctggcgtcgc tttcacgcag ggcgggaatg ccgaggcgcg gaatgccggg
     1981 cacgaagcca gccgacggga tcatatcggc gaccggcgcg cccggggtgc gatcggccca
     2041 gggcgggaaa tagccgtgga cgagtttcaa tttttcctcg cgcgtcatct gcgacacgag
     2101 gccggcggcg cgggaccgtg ccggcgactc gctcgacgcg gtcgaaagtc ccggcgcaga
     2161 aacgtgctga ac
//
LOCUS       DASVTM010000297         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_297053,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000297 DASVTM010000000
VERSION     DASVTM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297053"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VF633_08145"
     CDS             complement(<1..1016)
                     /locus_tag="VF633_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX8471067.1"
                     /translation="MTDAKTDSVKPTQKQMANAIRVLAMDGVEKAKSGHPGMPMGMAD
                     VATVLFSKFLKFDASRPDWADRDRFILSAGHGSMLIYALLHLTGYEAATKEQLSNFRQ
                     WGSKTAGHPEWGHMPGVEMTTGPLGQGLATSIGFAMAERHLAAKYGSDLVDHRTWVVA
                     GDGCLMEGVSQEAIALAGRYQLNKLTVLWDDNEITIDGKVSLSDAVDQKARFKASGWA
                     VKAVDGHDVDAIKSALKWATRQDKPTLIACKTKIGRGAATMEGSHKTHGAALGAAEIA
                     ATRLGLAWNHEPFVIPENIDKTWKKVGKQGSKARKAWEARLAASSQAADFTRAMAGDL
                     PERA"
     gene            1110..1367
                     /locus_tag="VF633_08150"
     CDS             1110..1367
                     /locus_tag="VF633_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471068.1"
                     /translation="MADATTAARDRVDRALAALERKVLDLKARPASAPSITDDDLFAS
                     VPADQARIAELEAAGREASAALARAADQLRGLLSTEQEADD"
     gene            1367..1690
                     /gene="zapA"
                     /locus_tag="VF633_08155"
     CDS             1367..1690
                     /gene="zapA"
                     /locus_tag="VF633_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX8471069.1"
                     /translation="MATVTVDINGRPYAVGCADGQEERVRILAKQFDSHVRSVAGDVG
                     HVGDIRLFLMAALLLADELHEARLALQSGTPVAAPIALGVASDGVAEALNAVAARLEK
                     LVQTI"
     gene            2027..2584
                     /locus_tag="VF633_08160"
     CDS             2027..2584
                     /locus_tag="VF633_08160"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX8471070.1"
                     /translation="MILDKPALRASMRALRKRLADADPLAAERAAEHIQHLPPGELVA
                     LYRAIGSELDTDAIARALIASGRTLCLPVVLERDAAMIFRLWSPGDPLEIDVAGCPAP
                     FPLAATVIPDLILTPLLAFDDLGARLGQGGGFYDRTFAVMPDTVRIGFAYEGQRLDSI
                     PMQAHDQRLHGVLTEVGYTRARKVP"
     gene            2588..3409
                     /locus_tag="VF633_08165"
     CDS             2588..3409
                     /locus_tag="VF633_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX8471071.1"
                     /translation="MRLAFFGDVIGKSGRDGISDHLPGLKRDLKLDFVVINAENAAGG
                     FGITENTARELFMAGADCLTLGNHSWDQREALTYIVREPRLIRPANYPRLMDAPGAGA
                     NLFETHSGKTVLVMNVLGRIHMDPMDDPFGAVDRELNAAPLGMVADAVIVDMHAEATS
                     EKMAMGHFCDGRASLVVGTHTHVPTADCQILPGGTAYQTDAGGCCDYDSVIGNEKEEP
                     LRRFTTRISQGRYAPAHGPATICGVFVETDDRTGLAIRVEPIRVGGRLSQAIPVV"
     gene            complement(3414..4979)
                     /locus_tag="VF633_08170"
     CDS             complement(3414..4979)
                     /locus_tag="VF633_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8471072.1"
                     /translation="MTRFDLSRRGLLTAFGGGLALTQTGPALAQAAFDVNPFRLGVTA
                     GDPLPDGFVIWTRLAPDPLAYGSGMPNAPMAVKWEVAADRGFNTVVRSGEAIARPELG
                     HSVHVEVEGLEPARAYFYRFQVGTERSGVGRAKTAPVIGAALAQAKFGVVGCQAYESG
                     YYTAHRKIAAEDLDFIFCYGDYIYEGRGNPVYQSGQGPQENARVHLGGEIYSLDDYRR
                     RYAQYTMDVDLQASRASAAWFAVYDDHEVDNNWVGDIDQDGTPPEVFLVRRAAAMQAF
                     YEFMPMRKSAFPRGSAMQLYRHAQYGDLMDLSFLDTRQFRSNQPCDDKFGASCAAIDA
                     SEAQVLGQAQEAWLFNNLDRSKATWKVLAQQVMMMDLDRVAGDGWGVNSDSWAGYSTP
                     RRRLLKHMQDRRVTNAVVLTGDEHQSFAGDLFLDGRSPEGAPIASEFVGTSISSGGDG
                     LDQRADWAAFMATNAQLKFLNSQRGYMICDVNKERWQSEFKVLDQIHDRSGVLTTRAK
                     YAIEAGKSGVVEA"
BASE COUNT          837 a         1705 c         1645 g          980 t
ORIGIN      
        1 aaggcgcgtt caggcagatc tccggccatc gcgcgggtga aatcggcggc ctgcgacgag
       61 gcggccagac gtgcttccca ggccttgcgg gccttggagc cctgtttgcc gaccttcttc
      121 caggtcttgt cgatgttctc cgggatcacg aatggctcgt gattccaggc caggcccaga
      181 cgcgtcgccg cgatctcggc cgcgccgagc gccgcgccat gcgtcttgtg gctgccttcc
      241 atggtcgccg cgccgcgacc gatcttcgtc ttgcaggcga tcagcgtcgg cttgtcctga
      301 cgggtcgccc atttcagcgc ggacttgatc gcgtcgacgt catgcccgtc gacggccttc
      361 acggcccagc cggaggcctt gaagcgagcc ttctgatcga cggcatccga caggctgacc
      421 ttgccgtcga tggtgatttc gttgtcgtcc cacagcacgg tcagcttgtt cagctgatag
      481 cggccggcca gcgcgatggc ttcctgcgac acgccttcca taaggcaacc gtcgccggcg
      541 acgacccagg tgcggtgatc gacgaggtcg gaaccgtatt tcgccgccag atggcgctcg
      601 gccatggcga agccgatcga ggtggcgagg ccctgcccca gcggaccggt cgtcatctca
      661 acgcccggca tatgacccca ctcggggtgg ccggcggttt tagagcccca ctgacggaag
      721 ttcgaaagct gttccttggt ggcagcctca tagcctgtca ggtgcagcag ggcgtagatc
      781 agcatcgagc cgtgacctgc cgacaggatg aaccggtcac gatccgccca gtccggacgc
      841 gaggcgtcga acttgaggaa tttcgaaaac agcaccgtcg ctacatcggc catgcccatc
      901 ggcatacccg ggtggccgct cttcgccttt tccaccccat ccatcgcaag cacgcggatg
      961 gcgttggcca tctgcttttg ggtcggcttc acggaatcgg tcttggcgtc ggtcatggaa
     1021 gacctttcgt cgatgggcgc taaaattcgg tgcgccgctt taccgcgagg cgtcgagggg
     1081 ttctataaga aaactggagg atgcgattga tggccgacgc cacgaccgcc gcgagggacc
     1141 gggtagaccg ggctctggcc gcacttgaac gtaaggttct ggacctgaag gcgagacccg
     1201 cctcggcgcc atcgatcact gacgacgatc ttttcgcttc cgtccctgcc gatcaggcgc
     1261 ggatcgcaga gctcgaagcg gccggtcgag aagcctccgc cgcactcgct cgcgcagccg
     1321 atcaactgcg tgggctgctg tccaccgaac aggaagctga cgactgatgg cgacggtcac
     1381 ggtcgatatc aacgggcgcc cctatgccgt cggttgcgcc gatggtcagg aagagcgcgt
     1441 ccgcatcctc gccaaacagt tcgatagcca cgttcgttcg gtggctggcg acgtcggcca
     1501 tgtcggggac atacgccttt tcctgatggc ggcgcttctg ctggctgacg aattgcacga
     1561 agctcgtctg gcgttgcagt cgggaacacc ggtcgcggct cccatcgcgc tgggcgtcgc
     1621 ctccgacggt gtggcggaag cccttaacgc cgtcgccgcc cgtctcgaga agctcgtcca
     1681 gacgatctaa atcctgcgcc tgccgcgctt gtagtgcggc gaggtagggc ctatcttcat
     1741 caacggcggg tcctgctggg tctcacgtcg aaggcaacac atcctcgcgg ccttaattca
     1801 ctcgaaggga gctgtccctg tctgggtccg tggacccggg tatatggcgc ccacctggct
     1861 acccaggctc gagaggatta aaccgtcctt cacggttatt ccggcgccgc caacctcctc
     1921 tcttttttag cgctatcgtt cgccgcgcgg cggctctctg cttgagcgca gcaggcccct
     1981 gcgctaccgc tcgcgggcgg cgacgggccg ttcagcggcg ttacccatga tcctggacaa
     2041 gcccgccctt cgcgcttcga tgcgcgctct tcgcaaacgt ctcgctgatg ccgatccgct
     2101 cgccgctgaa cgtgctgccg agcatattca gcatctgccg ccgggcgagc tcgtcgcact
     2161 ctatcgcgcg atcgggtccg agctggacac cgacgcgatc gcccgggccc tgatcgcctc
     2221 gggccggacg ctctgcctgc cggtcgtgct ggagcgcgat gctgcgatga tctttcggct
     2281 ttggtctccg ggcgatccgc tggagatcga cgttgccggt tgccccgcac cctttccgtt
     2341 ggcagcgact gtgatccctg acctgatcct gacaccactg ctggccttcg acgatttggg
     2401 cgcgcgactg gggcagggcg gcggcttcta cgaccgcacc ttcgccgtca tgcccgacac
     2461 cgttcgcatc ggcttcgcct acgagggcca gcgtctggac agcattccga tgcaggcaca
     2521 cgatcaacgt ctgcatggcg ttctgaccga ggtcggctat acccgggcgc gaaaggtccc
     2581 ataactcatg cgtctagcct tcttcggcga cgtgatcggc aagtccggtc gcgacggcat
     2641 cagcgatcat ctgcccggcc tcaagcgaga cctgaagctc gacttcgtgg tcattaatgc
     2701 agagaacgcg gcgggtggtt tcggcatcac cgagaacacg gcgcgggagt tgttcatggc
     2761 cggcgccgat tgcctgacgc ttggcaacca ctcctgggac cagcgtgagg ccctgaccta
     2821 tatcgtgcgt gagccgcgct tgatccgacc ggccaactat ccccggctca tggatgcgcc
     2881 cggcgccggc gcaaacctgt tcgagaccca ttcgggcaag acggtgctgg tcatgaacgt
     2941 gctgggccgc atccacatgg acccgatgga cgatccattc ggcgccgtgg accgcgagct
     3001 caatgcggct ccgctgggca tggtggccga cgccgtcatt gtcgacatgc atgccgaggc
     3061 cacctccgag aagatggcca tgggccattt ctgcgatggt cgcgcctcgc ttgtcgtcgg
     3121 gacgcacacc cacgtcccaa ctgcggattg ccagatcctg cccggcggca ctgcctacca
     3181 gaccgacgct ggcggctgct gcgactatga cagcgtcatc ggcaatgaga aggaagagcc
     3241 gctccggcgc ttcaccactc gtatctcaca gggccgctac gcacccgccc atggtccggc
     3301 tacgatctgt ggcgtcttcg tcgagaccga cgaccgcacc ggtttggcca tccgggtcga
     3361 acccatccga gttgggggtc gcctgagcca ggcgattccg gtcgtctgag tcctcaggct
     3421 tcgacgacgc ccgacttgcc cgcctcgatg gcgtattttg cccgcgtggt caggacgccg
     3481 ctgcggtcgt ggatctggtc cagaaccttg aactcggact gccagcgttc cttgttgacg
     3541 tcgcagatca tatagccgcg ctggctgttg aggaacttca gctgggcatt ggtggccatg
     3601 aaggcggccc agtcggcgcg ctggtccaga ccgtcgccgc ccgagctgat cgaggtgccc
     3661 acgaactcac tcgcgatggg cgcgccttcg ggagatcggc cgtcgaggaa caggtcgccg
     3721 gcgaaactct ggtgttcgtc tccggtcagg accacggcat tggtcacgcg ccggtcttgc
     3781 atatgcttca gcagccggcg gcgcggggtc gaatagcccg cccagctgtc ggagttgaca
     3841 ccccaaccgt cgcccgcgac ccggtcgaga tccatcatca tcacctgctg cgccaacacc
     3901 ttccaggtcg ccttggagcg gtcgaggttg ttgaacagcc aggcctcctg ggcctgtccc
     3961 aggacctggg cttccgaggc gtcgatggcg gcgcagctcg ccccgaactt atcatcacaa
     4021 ggctggttgg agcggaactg gcgcgtgtcc aggaagctca gatccatcag gtcgccatac
     4081 tgggcatggc gatacagctg cattgccgac ccccgcggga aggccgactt ccgcatcggc
     4141 atgaactcgt agaaggcctg catcgccgcg gcgcggcgca caaggaagac ctccggcggt
     4201 gtgccgtcct gatcgatatc gccgacccag ttgttgtcga cctcgtggtc atcgtagacc
     4261 gcgaaccatg ccgccgacgc gcgcgacgcc tgcaggtcca catccatcgt atattgcgcg
     4321 tagcgccggc ggtagtcgtc gagactgtag atctcgccgc caaggtgaac gcgggcgttt
     4381 tcctgcggcc cctgaccact ttgatagaca ggatttcctc ggccctcgta gatgtagtcg
     4441 ccgtagcaga agatgaaatc gaggtcctcg gccgcgatct tgcggtgggc ggtgtagtag
     4501 ccgctctcat aggcctggca cccgactacg ccgaatttgg cttgggccag agcagcgccg
     4561 atcaccggtg cggtcttggc tcggcccacg ccgctgcgct cggtcccgac ctgaaagcgg
     4621 tagaaatagg cgcgggcggg ctccagccct tcgacctcta cgtggaccga atggcccagt
     4681 tcgggacggg cgatcgcctc gcccgagcgc accacggtgt tgaagccgcg atccgccgca
     4741 acctcccatt tgaccgccat cggcgcgttc ggcatgcccg agccgtaagc cagtgggtct
     4801 ggcgcgaggc gcgtccagat gacgaagccg tcgggcaggg gatcgcccgc cgtcacaccg
     4861 agacggaacg ggttcacgtc gaatgccgcc tgagccagcg ctgggccggt ctgggtcagg
     4921 gcgaggccgc caccgaaagc ggtcaggagg ccacggcgtg acagatcgaa acgggtcata
     4981 tggcgcggcc tttgcggggt gatatttccc gcctacgcct cgattgcgac aatcgtgttt
     5041 agtcgccgat caggctgcga cgtctagcgc gtggttggtc cgcgcccgca atctcaggtg
     5101 aatcgggacc cggctccgga ggctgatctg gtgcgtcggg cggggttcga ccgtgctgat
     5161 cgcctcg
//
LOCUS       DASVTM010000298         6580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_29532, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000298 DASVTM010000000
VERSION     DASVTM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29532"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF633_08175"
     CDS             <1..241
                     /locus_tag="VF633_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX8471073.1"
                     /translation="ESKRLLGVLEGRLEGRDWIMGDQFTIADVSMIGWVRNLIGFYDA
                     GEIVDYARLKNVPAWLERALARPAVERGLSIPARG"
     gene            complement(245..430)
                     /locus_tag="VF633_08180"
     CDS             complement(245..430)
                     /locus_tag="VF633_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471074.1"
                     /translation="MAVAMHTPPAVIEIAQDGDRWRLNSSNRLFSGVFIDRRSALRHA
                     MAEAECHPGHVVVVRPG"
     gene            complement(430..657)
                     /locus_tag="VF633_08185"
     CDS             complement(430..657)
                     /locus_tag="VF633_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471075.1"
                     /translation="MMSGSISGTAPHPVMPRHYEVVARAGGWSIVLNGACTRPFRSRR
                     AAERIARTLQKQADALHGLAGHGMKRRGAGH"
     gene            complement(970..2274)
                     /gene="hslU"
                     /locus_tag="VF633_08190"
     CDS             complement(970..2274)
                     /gene="hslU"
                     /locus_tag="VF633_08190"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX8471076.1"
                     /translation="MTDLSPREIVSELDRFIVGQHDAKRAVAVALRNRWRRKRVPEDL
                     RDEVTPKNILMIGPTGVGKTEIARRLARLAGSPFLKVEATKFTEVGYVGRDVDQIMRD
                     LVEAALIMVRDARRSGVRAKAEAAAEERILDALVGPGSGPATRDSFRKKLRAGEMDDK
                     EIDIDLADTGSPMSGMDLGGAGGGIGMINLSDMLGKMGGPRTRSVHTTVKAAVAPLIT
                     EESDKLLDQDSLTRDAVTLAEQEGIVFLDEIDKVAGRQDRGGADVSREGVQRDLLPLI
                     EGTTVSTKYGPVKTDHVLFIASGAFHVAKPSDLLPELQGRLPIRVELKALTRDDFVRI
                     LTEPEANLIRQNQALLATEEVTVIFTPDAVEAMADAAVAANGAVENIGARRLVTVIER
                     VLEETSFKASDLSGQTLTFDGDQVREKIGDLAKNADLSRYIL"
     gene            complement(2492..2896)
                     /locus_tag="VF633_08195"
     CDS             complement(2492..2896)
                     /locus_tag="VF633_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471077.1"
                     /translation="MTDRITPVSAVTGGTATPGVTRVGDRRGSERRSAERRAKDEAKK
                     QVSTSRELVPVGKVVDNDPAGPAARKTPRTRAEMAAAAFAAQMLGQGGQRNGIKGGPP
                     VMDNARSTYLGAQYSGEHERRPPVGTNTRKDV"
     gene            complement(2893..3579)
                     /gene="phaR"
                     /locus_tag="VF633_08200"
     CDS             complement(2893..3579)
                     /gene="phaR"
                     /locus_tag="VF633_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX8471078.1"
                     /translation="MADDKPHTGKTKAGDVVVIKKYANRRLYNTSTSAYVTLEDLAQM
                     VRDGIEFVVFDAKTNDELTRQILTQIIFEEESRGEALLPVQFLRQLIGFYGGQMQGVL
                     PSYLEMSLESFSRQQEQYRAQVAKALTGGVPATGAIGGSLMEEAVKQNMAMFQNAMKM
                     FPAFGAYAARPEPEAATETAVHDAAPRTAPTSNTASDQLAEMRRQMDEMRAQIERIAT
                     PPSTKGPAGE"
     gene            complement(3689..4399)
                     /locus_tag="VF633_08205"
     CDS             complement(3689..4399)
                     /locus_tag="VF633_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX8471079.1"
                     /translation="MSMRLKSLFTHVCTPDELDMIDHYQTRTERLADLWVHVTGLSLA
                     AIGGIVLAGLAGVYGGLGTVMATAVYAICLIAMLTTSTIYNWTTPCAARPVLRRMDEA
                     AIFLMIAGSYTPFTTQRFEGMWAIGFTLAVWAIAFAGVAVKLVAPRISDAFWSGVYVL
                     FGWLAVVALKPMLQTVHPLALGLLVLGGLIYTAGVFVFISPRVKYRRAIWHGFVVAGA
                     GVHWVAVLVGVVLAPQMG"
     gene            4548..5735
                     /locus_tag="VF633_08210"
     CDS             4548..5735
                     /locus_tag="VF633_08210"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8471080.1"
                     /translation="MTSAVSDVVIVSAARTPVGSFMGALSSLPASKLGEIAIKAALER
                     AGVSGAEVDEVILGHVLQAAAGQGPARQASMGAGVPQETPAWSLNQICGSGLRAVALG
                     AQQIALGDAKIIVAGGQESMSQAPHAAHLRNGQKMGDLSFTDTMIKDGLWDAFNGYHM
                     GQTAENIASQNHITREAQDAFAVASQNKAEAAQKAGRFADEIVPVVIPGKKGDVTVDQ
                     DEYIRHGATLEAMEKLRPAFTKDGTVTAANASGLNDGAAALVLMSADDARARGLEPLA
                     RIASWATAGVDPAIMGTGPIPASKKALDKAGWTVADLDLVESNEAFAAQSLCVLKELG
                     LDAAKVNVNGGAIAIGHPIGASGARILTTLLFEMKRSGAKKGLATLCIGGGMGVAMCV
                     ERV"
     gene            complement(6051..>6580)
                     /locus_tag="VF633_08215"
     CDS             complement(6051..>6580)
                     /locus_tag="VF633_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8471081.1"
                     /translation="QLKLDEWVYQPGLPTNAVAPTSPDFDAVDRAAKAFFADRGAASA
                     IPWSGWSTQQRQRFLGWRPAGLRDGADWLTNQQLADLEKTLNLAKEGNSELVFGWLQI
                     AVAHRYQPAVPTLEHFLTSQGRRKFVMPLFTSLWNEGDWGRPLATQIYAKARPGYHPV
                     TTGSVDALVGKPAGQ"
BASE COUNT         1141 a         2175 c         2128 g         1136 t
ORIGIN      
        1 cgagtcgaaa cggttgctcg gcgtgctcga aggccgtctc gaaggccgcg actggatcat
       61 gggcgaccag ttcaccatcg ccgacgtctc gatgatcggc tgggtccgca acctcatcgg
      121 cttctacgac gccggcgaga tcgtcgatta cgcgagactg aagaacgtgc ccgcctggct
      181 ggaacgcgcc ttggcccgtc ccgccgtcga gcgcggcctg agcatcccgg cgagaggtta
      241 gacctcagcc aggcctgacc acgaccacgt ggccggggtg gcattccgcc tcggccatgg
      301 cgtgacgcag ggcgctgcgg cggtcgatga atacaccact gaaaagccgg ttgctcgagt
      361 tcagccgcca acgatcacca tcctgagcga tctcaatcac ggccggggga gtatgcatcg
      421 cgacagccat cagtgaccag cgccccggcg tttcatgccg tgccccgcta gtccatggag
      481 ggcgtcggcc tgcttctgca gcgtgcgggc gatccgctct gctgcacgac ggctgcggaa
      541 gggccgggtg caggcgccgt tcagaacgat gctccatcca cccgcccgcg ccaccacttc
      601 atagtgtcga ggcatgacgg gatgcggcgc ggtcccggag atgctgccgg acatcatcgg
      661 ccgatcctcc gcgtgccgcc ctgcgactgt gatccagagc gtggcccaga gcgtagccct
      721 gccccaaaag ccgagccgag aaccgccagc gccatgcgca agctgctcca tcgctgaggc
      781 tccgggatcc ggggtgactg tgtcgtgcgc ggcatggggt gtcctttcgc ggtcagttca
      841 ccgcaaaggc ccataacgaa gccattccga atatcgaccc gcgcataacg gcgacgtaaa
      901 gttcatgaga aaaaggcctc gcggatgaac cgcgaggcct ctgaacgtca cgatatctct
      961 acgtctccgt cagagaatat accggctcag gtcggcgttc ttggccagat caccgatctt
     1021 ctcgcgtacc tgatcgccat cgaacgtcag ggtctggccg gacaggtccg aggccttgaa
     1081 agaggtctcc tccagcaccc gttcgatcac ggtgacgagg cggcgcgcgc cgatgttctc
     1141 gaccgcgcca ttggccgcca cggccgcatc ggccatggcc tcgactgcgt cgggggtgaa
     1201 gatcaccgtc acctcctcgg tggccagcag ggcctggttc tggcggatca ggttggcttc
     1261 gggctcggtc aggatacgaa cgaagtcgtc acgggtcagt gccttgagct cgacccggat
     1321 cggcagccgc ccctgcaact cgggcagcag gtccgacggc ttggccacgt gaaaggcgcc
     1381 cgaggcgatg aacagcacgt ggtcggtctt caccggtcca tatttggtcg agacggtggt
     1441 gccctcgatc agcggcagca ggtcgcgctg cacgccctcg cgcgacacgt cggcgccgcc
     1501 ccgatcttgc cgccccgcaa ccttgtcgat ctcgtcaagg aagacgatcc cttcctgctc
     1561 ggccaaagtc accgcgtcgc gggtcaggct gtcctgatcc agcagcttgt cgctttcctc
     1621 ggtgatcagc ggcgccactg ccgccttgac cgtggtgtgg accgacctgg tgcgcggccc
     1681 gcccatcttc cccagcatgt cggacagatt gatcatcccg atcccgccgc cggcgccgcc
     1741 cagatccatg cccgacatcg gcgacccggt gtcggccaga tcgatgtcga tctccttgtc
     1801 gtccatctct ccggcgcgga gtttcttgcg gaagctgtcg cgcgtcgctg gacctgagcc
     1861 ggggcctacg agggcgtcga gtatccgttc ctcagccgct gcctcggcct tggcgcgtac
     1921 gccgctgcgc cgggcgtcgc gcaccatgat cagcgcggcc tcgacgagat cgcgcatgat
     1981 ctgatcgacg tcgcgtccga cgtagccgac ctcggtgaat ttggtcgcct cgacctttag
     2041 gaagggcgaa cccgccagcc gcgccagacg cctggcaatc tcggttttgc cgacgccggt
     2101 cgggccgatc atcaggatgt tcttgggcgt gacctcgtcg cgcaggtctt cgggtacacg
     2161 cttgcggcgc cagcggttgc gcagggccac ggcgacggcg cgcttggcgt cgtgctggcc
     2221 gacgatgaag cggtcgagtt cggaaacgat ttcgcgggga gacagatcgg tcataccgac
     2281 cagatagggc gtctcacagc cgagcgggag aggtgagccc caaacgctga ccagttgtcg
     2341 cggctcttac agcaggacgg cgaacaccag cgcgacgacc acgacatcga cgatgcggaa
     2401 ggcggagttg aaggcgaggg acatggcgga accactggat cgggacagat accttgttca
     2461 ctgcaaaggt cgcgccagat tgatccgccc gtcagacgtc cttgcgcgtg ttcgttccga
     2521 ccggcgggcg tcgctcgtgc tcgccggaat actgggcccc gagataggtg gaacgggcgt
     2581 tgtccatcac cggcggaccg cccttgatgc catttcgctg tccgccctga cccagcatct
     2641 gggcggcgaa ggcggctgcc gccatctcgg cccgggtgcg cggcgtcttg cgggcggcag
     2701 ggcctgccgg gtcattatcg acgaccttgc ccaccggcac gagttcgcgg gaagtgctga
     2761 cctgcttctt ggcctcatcc ttggcccgcc gttccgcgga gcggcgttcc gatccccgcc
     2821 tgtcacccac gcgcgtcacg cccggcgtcg ccgtaccgcc cgtgacggcg ctgaccgggg
     2881 tgatacgatc ggtcattcgc cggcagggcc cttggtgctc gggggggtgg cgatgcgctc
     2941 gatctgcgcg cgcatctcgt ccatctgacg acgcatctcc gccagctgat cgcttgcggt
     3001 gttcgaagtc ggggctgttc tgggcgcggc gtcatggacc gcagtttccg tggctgcttc
     3061 aggctcgggc cgtgcggcat aggcgccgaa ggccgggaac atcttcatgg cgttctggaa
     3121 catcgccata ttctgtttga cagcttcttc catcaagctg ccgccgatcg cgccggttgc
     3181 aggaacgccg cccgtgaggg ccttggcgac ctgggcgcgg tactgttcct gctggcgcga
     3241 gaagctctct aacgacattt ccagatagct cggcaggaca ccctgcatct ggccgccgta
     3301 aaagccgatc aactggcgca ggaactggac gggcagcagc gcctcgccgc ggctctcttc
     3361 ctcgaagatg atctgggtca ggatctgacg cgtcagttcg tcattggtct tggcgtcgaa
     3421 cacgacgaac tcgataccgt cgcggaccat ctgggcgagg tcctcgagcg tcacataggc
     3481 gctcgtggag gtgttataga gacggcggtt cgcgtatttc ttgatgacga ccacgtcgcc
     3541 cgccttggtc ttgcccgtat ggggtttatc gtcggccacc tgtcgagctc cctaaactct
     3601 tttcctcagt atcatgcagc gcgaagacgg acgccagcgc aatatttcgc gctgcaccaa
     3661 gcgcgaatag ctgagtggag ccaaggggct aacccatctg gggggcaagg acgacaccga
     3721 ccagcacggc gacccagtgc actccggccc cggcgacgac gaagccatgc cagatggctc
     3781 ggcggtattt cacccgcggg cttatgaaga cgaacacccc ggcagtatag atcagcccgc
     3841 ccagcaccag cagccccaga gccaggggat ggaccgtctg cagcatcggc ttcagcgcca
     3901 ccacggcaag ccagccgaac aggacataga cgccgctcca gaacgcgtcg gagatacgcg
     3961 gggctaccag cttcaccgcg acgccggcga aggcgatagc ccagaccgcg agggtaaagc
     4021 cgatggccca catgccctca aacctctggg tcgtgaacgg tgtgtagctg cctgcgatca
     4081 tcagaaagat cgcggcctcg tccatgcgcc tcagcaccgg ccttgccgcg cagggagtgg
     4141 tccagttgta gatggtcgac gtcgtcagca tggcgatgag gcagatggca tagacggcgg
     4201 ttgccataac cgtccccagg ccgccgtaca ccccggccag cccagccaga acgatgccgc
     4261 cgatggccgc cagtgacagg cccgtcacat ggacccacag atccgccaga cgctcggtgc
     4321 gcgtctggta gtgatcgatc atgtccagtt cgtccggcgt gcagacgtgt gtgaacaggg
     4381 atttcagacg catactcatg gctccatcaa gcctgcgacc gtgcggttcg ttccctcatt
     4441 ctcgtcgaga cacgatgacc attggctgaa taccgcttaa atgccgtgac gaaggcggcc
     4501 ggatgcggca gaaacgccac gcaatatttc agcagtgagt gaatatcatg accagcgctg
     4561 tctcggacgt cgtcatcgtc tccgccgccc gcacccctgt cggctccttc atgggcgccc
     4621 tgtcgagcct gcctgcgtcg aagctgggcg agatcgcaat aaaggcggcg ctggagcgcg
     4681 caggcgtcag cggcgcagag gtcgacgagg tcatcctcgg ccacgtgctt caggccgcag
     4741 ccggtcaggg cccggcccgt caggcctcga tgggcgcagg cgtcccgcag gagaccccgg
     4801 cctggagcct gaaccagatt tgcggttccg gcctgcgcgc cgtcgccctc ggcgcccagc
     4861 agatcgcgct cggggatgcg aagatcatcg tcgccggtgg tcaggaaagc atgagccagg
     4921 cgccccacgc cgcccacctg cgcaacggcc agaagatggg cgacctgtcg ttcaccgaca
     4981 ccatgatcaa ggacggactg tgggacgcct tcaacggcta tcacatgggt cagaccgccg
     5041 agaacatagc ctcgcagaac cacatcaccc gcgaagccca ggacgcgttc gcggtcgcct
     5101 cgcagaacaa ggccgaggcc gctcagaagg ccggtcggtt cgccgacgaa atcgttccgg
     5161 tggtgatccc tggcaagaag ggcgacgtca ccgtcgatca ggacgaatac atccgccacg
     5221 gcgccacgct cgaggcgatg gagaagctgc gccccgcctt caccaaggac ggcaccgtca
     5281 ccgccgccaa cgcttcgggt ctcaacgacg gcgctgcggc tctggtgctg atgtcggctg
     5341 atgacgccag agcgcgtggg ctcgagcctc tggcccggat cgcctcgtgg gccacggcgg
     5401 gcgtcgatcc ggcgatcatg ggtacgggtc caatcccggc ttcgaagaag gcgctcgaca
     5461 aggccggctg gaccgtcgcc gatctcgatc tggtcgaatc caacgaagcc ttcgccgccc
     5521 agtcgctgtg cgttctgaag gaactcggcc tcgacgctgc gaaggtcaac gtcaacggcg
     5581 gcgccatcgc catcggccac ccgatcggag cctccggcgc acgcatcctg accacgctgc
     5641 tgttcgagat gaagcgttcc ggcgccaaaa aaggcctcgc caccctctgc atcggcggcg
     5701 gcatgggcgt cgccatgtgc gtggaacggg tctaaccgcc cttcttcggg tcgagtgtct
     5761 ccacgtccgc agaaatgcga aaagggagac actcgaccct gagcgttgtg gggatcgagc
     5821 tgcgcgggcg cagccgtcag acggttattc gcgaccctgt agcgggccgc gaggagcggg
     5881 ttttcaaaga ccgggctaga atccggcgtg tgtccggatt ccagaagtca ttgtacgtgc
     5941 cgccaaagcc ctgatgcgtc gatttccggg acgggggtcg agtgtctcca gtccggggta
     6001 tcgaagggac gagagtatca gggtcgagag tctccgctga tgcctgcgcc ctattgcccc
     6061 gccggcttcc cgaccagggc atccaccgaa cccgtcgtga ccgggtgata ccccgggcga
     6121 gccttggcgt agatctgcgt cgccagcggg cggccccaat cgccttcgtt ccacaggctg
     6181 gtgaacagcg gcatgacgaa tttccggcgg ccttgcgagg tcaggaaatg ctccagcgtc
     6241 ggcacagccg gctgatagcg atgggcgacc gcgatctgaa gccagccgaa caccagttcg
     6301 gagttgccct ctttcgcgag gttcagcgtc ttctccagat cggccaattg ctggttggtc
     6361 agccagtcgg cgccgtcacg caggcccgcc ggacgccagc cgaggaagcg ttggcgctgt
     6421 tgagtgctcc agccgctcca cgggatggcc gaggccgcgc cacggtcggc gaagaaggcc
     6481 ttggccgcgc ggtcgacggc gtcgaagtcc ggtgacgtcg gcgccacggc gttggtcggc
     6541 aggcccggct gatagaccca ctcgtcgagc ttcagctggg
//
LOCUS       DASVTM010000299         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_189687,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000299 DASVTM010000000
VERSION     DASVTM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189687"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VF633_08220"
     CDS             complement(<1..403)
                     /locus_tag="VF633_08220"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX8471082.1"
                     /translation="MNLILFGPPAAGKGTQAKRLVEERGMVQLSTGDMLREAIASGSE
                     LGKQCQSIMSNGGLIADDIVIALIEARLKEAEDAGGAIFDGFPRTLGQAEALDAMLAK
                     LGKKIDHVVRLKVDDAALLERISKRYADQGRP"
     gene            complement(469..1848)
                     /gene="secY"
                     /locus_tag="VF633_08225"
     CDS             complement(469..1848)
                     /gene="secY"
                     /locus_tag="VF633_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX8471083.1"
                     /translation="MASAAEQLAANMNMGSFAKATDLHKRLLFTLLALLVYRIGTYVP
                     IPGINSEAFLQFFQNPDGKRGILDMFNMFSGGAVERMAVFALNVTPYISASIIVQLMG
                     TVYPPWEKLRKEGGESGRKTLNQYTRYLTVFLALAQSLGIAAGLNAQAGLIDQPGIFF
                     IISTVTCLTGGTMFLMWLGEQVTTRGIGNGISLIIFAGIVAVLPGTVARLFGLAQQGQ
                     LSAFALLAIALLAAATVVFIVFMERAQRRLLIQYPKRQEGNRMAGGERSFLPLKVNTA
                     GVLPPIFASSLLMLPSTAATMLVTADLPNWLNWLPMVTAQLTHGQPLFMVLYAALIIF
                     FCFFYTSITFNPEDTAENLRKYGGFLPGVRPGKRTAEYLDYVLTRLTVIGAAYITAVC
                     LIPEAIAASMGPSLLFGGTSILIVVSVTMDTVAQIQSQLLAHQYEGLIKKAKLRGRGG
                     RGAPTPVRR"
     gene            complement(2124..2570)
                     /gene="rplO"
                     /locus_tag="VF633_08230"
                     /pseudo
     CDS             complement(2124..2570)
                     /gene="rplO"
                     /locus_tag="VF633_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(2765..2944)
                     /gene="rpmD"
                     /locus_tag="VF633_08235"
     CDS             complement(2765..2944)
                     /gene="rpmD"
                     /locus_tag="VF633_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX8471084.1"
                     /translation="MATVTVKQTGSPIRRKNDQRATLVGLGLNRMGRESTLEDTPSVR
                     GMIAKVAHLTQIVEK"
     gene            complement(2948..3568)
                     /gene="rpsE"
                     /locus_tag="VF633_08240"
     CDS             complement(2948..3568)
                     /gene="rpsE"
                     /locus_tag="VF633_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX8471085.1"
                     /translation="MAQTPQRGGGGNDRNRRDNRNGPAADGPDSDIVEKLVHINRVAA
                     TVKGGRRFSFAALMVVGDGKGRVGFGHGKAREVPEAIRKATEEAKKTMIRVPLRENRT
                     LHHDGNGRWGAGKIMVRAAPPGTGVIAGGPMRAVLETLGVHDVVAKSTGSSNPYNMIR
                     ATFEALKVQSSPRQVASKRGKKVADLMGRRNDGASAPAETAETVEG"
     gene            complement(3581..>3730)
                     /locus_tag="VF633_08245"
     CDS             complement(3581..>3730)
                     /locus_tag="VF633_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX8471086.1"
                     /translation="EAAAQIGKLVAERAIEKGVTDVVFDRGGYIYHGRVKALADAARE
                     AGLNF"
BASE COUNT          664 a         1159 c         1226 g          681 t
ORIGIN      
        1 cggggcggcc ctggtcggca tagcgcttcg agattcgctc cagcagcgct gcatcatcga
       61 ccttgaggcg gacgacgtgg tcgatcttct tgcccagctt ggccagcatg gcgtcgagcg
      121 cctctgcctg ccccagggtg cgcgggaagc catcgaatat ggccccccct gcgtcttcgg
      181 cttctttcag acgggcctcg atcagggcga tgacgatgtc atcggcgatc aggcccccat
      241 tcgacatgat cgactggcac tgcttgccga gctcagagcc tgacgcgatg gcctcgcgca
      301 gcatgtcgcc ggtcgagagc tggaccatgc cgcgctcttc gaccagacgc ttggcctgcg
      361 tgcctttgcc ggcagccggc ggtccgaaca gaatcaggtt catagccgcc ccctagtcat
      421 cacgacgggc taaagtagcg cgaagcgcga tagcccaaac tcaatttatt accggcgtac
      481 gggagtcggt gcgccccgtc caccacgacc ccgcagtttg gccttcttga tcaggccttc
      541 gtactggtgg gccagcaact gcgactggat ttgcgcgacc gtgtccatcg tcaccgacac
      601 cacgatcagg atcgaggtgc cgccgaacag caggctgggg cccatcgagg cggcgatcgc
      661 ttcggggatc agacagacgg cggtgatata ggccgcgccg atcaccgtca gacgggtcag
      721 gacgtagtcc agatattccg ccgtgcgctt gcccggacgg acgcccggca ggaagccgcc
      781 gtatttacgc aggttctcgg ccgtgtcctc ggggttgaag gtgatcgagg tgtagaagaa
      841 gcagaagaag atgatcaggg cggcgtagag caccatgaac agcggctggc cgtgggtcag
      901 ctgagcggtg accatcggca gccagttcag ccagttcggc aggtcggcgg tgaccagcat
      961 ggtcgccgcc gtcgacggca gcatcagcag ggacgaggcg aagatcggcg ggaggacgcc
     1021 ggcggtgttg accttcaacg gcaggaacga acgttcgccc ccggccatcc ggttcccttc
     1081 ctggcgcttc ggatactgga tcagcaggcg gcgctgggcg cgttccatga agacgatgaa
     1141 gacgacggtt gcggcggcca gaagcgcgat ggccagaagg gcgaaggccg acagctgacc
     1201 ctgctgggcc aggccgaaca aacgcgccac ggtgccgggc agaacggcca cgataccggc
     1261 gaaaatgatc aacgaaatac cgttgccgat gccgcgcgta gtcacctgct caccaagcca
     1321 catcaggaac atcgtgccgc cggtcaggca ggtcacggtc gagatgatga agaagatgcc
     1381 aggctggtcg atcagaccgg cctgggcgtt gaggcctgca gcgataccga gcgactgggc
     1441 cagggcgagg aagaccgtca ggtagcgggt gtactggttg agggtcttgc ggccgctttc
     1501 gccgccttcc ttgcgcagtt tttcccacgg cggatacacg gtgcccatca gctgcacgat
     1561 gatcgacgcc gaaatgtagg gggtgacgtt cagggcgaag accgccatcc gctccacggc
     1621 gccgcccgag aacatgttga acatgtccag gatgccgcgc ttgccgtccg ggttctggaa
     1681 gaactggagg aaggcttcgg aattgatccc cgggatcggc acgtaggtgc cgatgcggta
     1741 aaccagaagc gccagaagcg tgaagagtag ccgcttatgc aggtcggtcg ctttcgcaaa
     1801 cgaccccatg ttcatattgg cggcaagttg ttcggcggcc gaagccatag atcgttaccc
     1861 cgactgatca gaacgcggcg tggatctcaa agtcccgagg gacagagaat ccagcgccac
     1921 tgtccagata ggcgaagggc ggcctcttag cgagaccgcc cctcaagctt tattcgttag
     1981 gccttggcag ccgtacgggt ggtacgggcc gagatcttgt tggcgtcgcc gcgcggagcc
     2041 ttggcagggg gaaccttgcc cttggccaga ttgcgcttct cgacggcggc ggcagccttg
     2101 gcttcagcag cgatgcgctg ttgaacgacc gaaccaccag cagcctcgat ggccttgatg
     2161 gcgccggccg aggccgacca gacaaccagg ttcagggctt gcttcagctc accggtgccc
     2221 agaacgcgga cgccgtcctt gacgcggcgg atgacgccgg cggcgaccag ggcatcgccc
     2281 ttgagctcgc tcgtagcgtc cagcttgcct tcgtcgatgg cgtcttgcag gcgccacagg
     2341 ttcacttcag ccagcttcag cgcgttcgga ttgttgaagc cacgctttgg catacgcatg
     2401 tacagcggca tttggccgcc ttcgaacccg ccgatggcga cgcccgaacg tgacttctga
     2461 cccttgacgc cacggccggc tgtcttgccc ttgcccgaac cggggccacg gccgacgcgc
     2521 atgcgcttct tgtgggcgcc ctgattgtcg cggatttcat tcaatttcat atgcctgatc
     2581 ctttcgggtt ctagcgcccg acgcgcgccc ccgaagggga atggcgtcgg actcggctgt
     2641 ttttcgatgt gaggactgaa aacgatcaga ccccggaacg cgatgaaggg cggcctatag
     2701 ccgccctcac cctgtcaccg caagggcgac agcctctccc ttgggggaga ggctggatat
     2761 gcacttactt ttcgacgatc tgggtcaggt gggcgacctt ggcgatcatc ccacgaaccg
     2821 agggggtgtc ttccagcgtg gactcacggc ccatgcggtt cagacccagg cccaccagag
     2881 tggcgcgctg gtcgttcttg cggcggatcg gcgaaccggt ctgcttgacg gtgacggtgg
     2941 ccatctatca gccctccacg gtttcggcgg tctcggctgg agccgaggcg ccatcgttgc
     3001 ggcggcccat caggtcggcg accttcttgc cacgcttgga agcgacctga cgcggcgacg
     3061 actggacctt gagggcctcg aacgttgcgc ggatcatgtt gtaggggttc gaagagccag
     3121 tggacttggc cacgacgtca tggacgccga gggtttccag aacagcacgc atcggaccgc
     3181 cggcgatgac gccggtgccg ggaggcgccg cgcgaaccat gatcttgccg gcgccccaac
     3241 ggccgttgcc gtcatggtgc aaggtgcggt tctcacgaag aggaacgcgg atcatcgtct
     3301 tcttggcttc ttcggtcgcc ttgcggatgg cttccggcac ttcgcgcgcc ttgccgtgac
     3361 cgaagccaac gcggcccttg ccgtcaccga cgaccatcag ggctgcgaac gagaaccggc
     3421 gaccgccttt gacggtggcg gcgacgcggt tgatgtggac cagtttctcg acgatgtcgg
     3481 aatccgggcc gtcagcagcc gggccgttac gattgtcgcg acggttgcgg tcgttgccgc
     3541 cgccgccacg ttggggtgtt tgcgccatct gtcgcgtccc ttagaagttc aggccggctt
     3601 cacgcgcggc atcagccagc gccttcaccc gtccgtgata gatgtagccg ccgcgatcga
     3661 agacgacgtc ggtgacgccc ttttcgatgg cgcgttcagc gaccagtttg ccgatctggg
     3721 cagcagcctc
//
LOCUS       DASVTM010000300         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_399052,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000300 DASVTM010000000
VERSION     DASVTM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399052"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VF633_08250"
     CDS             complement(<1..1001)
                     /locus_tag="VF633_08250"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8471087.1"
                     /translation="MATLTEVERDAIEGRPAETAPGPGPSVDFGALFEASSTPFLVVA
                     PPEWTIAAANGAYLRATGTTREVIGRRLFDVFTDDPEDPSADGVRNVDASLNRVVTTR
                     AADTMAVQRYAVQGQDGRFVERWWAPVNTPVLGKDGDVALILHHVEDVTEVIRLRGDA
                     EGRDQLARGQQAVIDRLRTYEAALRESEEFNRRVLQSSADCIKVLRLDGRLERMSEGG
                     QRALGITDMAAYLDANWAESFEPDWRAAAHAAVREAANGRTSRFEGALTTTLGELRWW
                     DSLLTPISGADGRPERVLAVSRDITDLRVGRDRMHESETRFRNMADNAPVMMWVTDP"
     gene            complement(1567..2520)
                     /locus_tag="VF633_08255"
     CDS             complement(1567..2520)
                     /locus_tag="VF633_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX8471088.1"
                     /translation="MNIHQNARLTPSGRERLVRLARSGLAPKAVAETMGVCVKTVRKW
                     VARFAAEGATGLQDRSSRPHTLHRPTPAETQESIVAQRRQRLTGQQIARDLAVSPATV
                     SRVLRRHGLSRIRDLEPPAPVQRYERERPGELIHIDIKKLGRFERVGHRITGERTGQS
                     KGRGTGWEYLHLAVDDHSRLAYSEIHPDEKRVSCLTFLFNALRFFRRHGIAVERVMTD
                     NGSAFKSRRYAKALRWLGIAHKRTRPYTPKTNGKVERFVQTSLREWAYARAYDTSEQR
                     RQELPAFLFRYNWQRPHMGINRQTPISRLGLSEDNLLMLHT"
BASE COUNT          440 a          918 c          916 g          498 t
ORIGIN      
        1 ctcggatcgg tcacccacat catcaccggc gcgttgtcgg ccatgttgcg aaagcgcgtt
       61 tcgctctcgt gcatccgatc ccggcccacg cgtaaatccg tgatgtcccg cgacaccgcc
      121 agcacgcgct cgggccgtcc gtccgcgccc gagatcggcg tcaggagcga gtcccaccag
      181 cgaagctcgc ccaaggtcgt cgtgagtgca ccctcgaagc ggctcgttcg tccgttcgcg
      241 gcttccctca cggcggcatg ggccgccgcg cgccagtccg gctcgaagct ttcggcccag
      301 ttggcatcca ggtacgcggc catgtcggtg atgccgagcg cgcgctgacc gccctcgctc
      361 atcctctcca ggcgcccgtc gagacggagc accttgatgc aatcggccga gctttggagg
      421 acgcggcgat tgaattcctc gctctcgcgc agggccgctt cgtatgtgcg cagccggtcg
      481 atcacggcct gctggccgcg ggccaactga tcccgccctt cggcgtcgcc gcgcagccgg
      541 atcacttcgg tgacgtcttc gacatggtgg aggatcaggg caacgtcgcc gtccttgccg
      601 agcactggcg tgttgaccgg ggcccaccac cgttcgacga agcgcccatc ctgtccttgc
      661 acggcatagc gctgaaccgc catcgtgtcg gcagccctcg tggtaaccac ccgattcagt
      721 gaggcgtcga cattgcgcac gccgtcggca ctcggatcct ctgggtcatc cgtgaagacg
      781 tcgaacagcc tgcgtccgat gacctcgcgc gtcgtacctg ttgcccgaag gtaggcgccg
      841 ttggcggcag cgattgtcca ttcgggcggg gccaccacga ggaagggcgt ggacgaagcc
      901 tcgaacagcg ctccgaagtc gacggatgga cctgggccgg gggcggtttc cgcagggcgc
      961 ccctcgatgg cgtcgcgttc cacctcggtc agggtcgcca tgccagactg cttgacgatg
     1021 gccgccgacg gatcaggttc gttcaaggcg ccaccgtctc attgaggcaa gggatctccg
     1081 cacgtccatt cacacgcacg acaggacctg tcggtgatgc tgcctaacta gcgcattggg
     1141 cagccaggcc tgtacgccgc cgccttgaac ggcccggcgg gtagaaaaga tcctgtttcc
     1201 cgtgggacac agcgaatgtt tcttgttcgt tccgccgatg gcggtagtgg aatggcgcgg
     1261 gcgaccgggc gtaattgagg agtcccctct cgtgccaggc acgcccatgc gaatgaccgc
     1321 caacgcgata tcccgcgtcg tgacgggagt accggcatct gggagagccc tcttcgtcag
     1381 ccggccgaag gcatcctaaa tatgctcctg gccggcaagc gtcgtctgag cgaacgcgcc
     1441 ggcagcttgc ggcgcgaacc gacctgacag agcaccggcc tcaccccgca ccgccgaacg
     1501 cgctcgtcat cgcctcgccc tcgacctcga ggcgagccac cttcgggtgg tcatgagccg
     1561 ccccgtctag gtgtggagca tcaacaggtt gtcctcggat aggccgagtc ggctgatggg
     1621 tgtttggcgg ttgatgccca tgtgaggccg ctgccaattg tagcgaaaga ggaaggccgg
     1681 gagttcctgt ctgcgttgct cggacgtgtc gtaggcacgg gcataggccc attcgcgcaa
     1741 ggacgtctgg acgaagcgct cgaccttgcc gttggtcttt ggggtgtagg gccgggtgcg
     1801 tttatgggcg atgcccagcc atcgcaaggc tttggcgtaa cgcctggatt tgaaggctga
     1861 cccgttgtcg gtcatcacgc gctcgaccgc gatgccatgg cggcggaaga agcgcagagc
     1921 gttgaagaga aaggtcaggc aggagacccg cttctcgtcg ggatggatct cggagtaggc
     1981 gagccgggaa tgatcgtcga cggccagatg caggtactcc catccggtcc cgcggccctt
     2041 gctctgaccc gtgcgctcgc cggtgatgcg atgtcccaca cgttcgaagc gcccgagctt
     2101 cttgatgtcg atgtggatca gttcgcccgg ccgctcgcgt tcgtagcgct ggacgggcgc
     2161 gggtggttcg aggtcgcgga tgcggctcag gccgtgccgg cgcagcaccc ggctgacggt
     2221 ggccggcgag acggccagat cacgggcgat ctgctggccg gtcaggcgct gccgacgctg
     2281 ggccacgatg ctctcctgcg tctcggctgg ggtcggtcga tgcagggtat gaggccgtga
     2341 cgagcggtcc tgcagcccgg tcgcgccctc ggctgcgaag cgggccaccc acttgcgcac
     2401 cgtcttgacg cacaccccca tcgtctcggc caccgccttc ggagcgagcc cgctccgggc
     2461 cagccgaacc agtcgctctc gacccgacgg cgtcaatcgc gcattctgat ggatgttcat
     2521 ccggtccctc cgggatagct gaagcctcgc aactccagcg tccccggttc agaccggatg
     2581 gacaacctcc tgaaagctca catctagtca gcctgtctac aatcgagatc catcgccgag
     2641 caggcacgct gggcggcagg gaaggtcgtc gtgagactcg cgatcgcagc acggatgagc
     2701 atcgccccga atgcggcggc ggggctcctc ggcccttcgc tcgtacgaaa cgctggtgtg
     2761 gaccgtcttg gg
//
LOCUS       DASVTM010000301         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_878351,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000301 DASVTM010000000
VERSION     DASVTM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878351"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..771)
                     /locus_tag="VF633_08260"
     CDS             complement(172..771)
                     /locus_tag="VF633_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8471089.1"
                     /translation="MNIAILGKGNMGAPLAELMEKAGHSVTRLGRHDDAVPALNTAAL
                     VVLALKYEQVLDLAGKPGVVAALSGKIVLDMTNPLAADYMSLTIGHSTSAAEEIAKRL
                     PDAHVVKAFNTVFAGLLARRAAGENVGVPVFVAGDDAGSVDIVLSLATSIGFEALNGG
                     SLSNARYLEPMAEFMIQLAYGLGHGAEIGFAVVKRCKAR"
     gene            869..1621
                     /locus_tag="VF633_08265"
     CDS             869..1621
                     /locus_tag="VF633_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8471090.1"
                     /translation="MALVDPDGHPSPVFVLSDKHPSMDGDWHSHRRLQLVHVSDGAVT
                     VITRQGRFVVPRQRAVWIGPRVEHRISSPAPFWLTTCYIEPNLLSVPNPATVVAVDRL
                     TDELLIAASGLEGGHPAVGPEARLIAVLLDRLPGLELADIFLPEPGDPKLRRLCSMLL
                     AEPSRSENLGALAGLAGMTERTAARLFVSDTGMTFGQWRQCLRLQIAFEKLGAGASVT
                     EAAFEIGYRDVSSFITAFKARFGGTPAKFVKR"
     gene            complement(1611..2054)
                     /locus_tag="VF633_08270"
     CDS             complement(1611..2054)
                     /locus_tag="VF633_08270"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8471091.1"
                     /translation="MSVLAFADGAAAGLVNAFEGFSTFKCAPVLNVHDVAVLPQYRGL
                     GLARRMLHLVEVLAIERNCCKLTLEVLEGNQPAKALYRSIGFTDYSADVATGQALFWE
                     KSLSGVQDVAGLSSLPRSGLSNGAAGFDRENDQAPQTEVQRVVSV"
     gene            complement(2379..>2784)
                     /locus_tag="VF633_08275"
     CDS             complement(2379..>2784)
                     /locus_tag="VF633_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471092.1"
                     /translation="SISLNAKPITDGALFLVEVDSHAMADADDRYAAHLIFGGKTAPV
                     ENAKATNISSFYADSFGGDFTALGAGSAHQGGFEVAYAYPDASFVNRWTVAPTTASWT
                     IIARDSAGSEEPFAAYELARTECTRSGPAERL"
BASE COUNT          534 a          910 c          846 g          494 t
ORIGIN      
        1 gcccgtcatg ccgcggccgg agagcgcggc atgacgggcg acgatcccct cctcccgcac
       61 tcgaacagga tgacgggcgc ccggctggac gagctaccgc gaaggcgcgg ccctctcgcg
      121 aggcgagagg acgctcgtgg agggcaagac aaacggcgca ggcttgcctg gctaacgagc
      181 cttacaccgc ttgacgacgg cgaacccgat ctcggcgcca tggccaagtc cataggcaag
      241 ctggatcatg aactcagcca taggttcgag atagcgcgcg ttcgaaagcg acccgccatt
      301 caaagcctcg aagccgatcg aggtcgccag cgaaaggacg atatccaccg aacctgcgtc
      361 gtcgccggcc acaaagacag gaaccccgac gttttcaccc gcggctcgtc tcgcgagaag
      421 gccggcgaac accgtattga acgccttgac cacatgagcg tcaggcaggc gcttcgcgat
      481 ctcctcggcg gcggaggtcg aatggccgat cgtcaggctc atatagtcag cggccagcgg
      541 gttggtcata tcaaggacga tcttgcccga cagggcagcg accactcctg gtttgccagc
      601 gaggtcgagc acctgctcgt atttgagagc gagcaccacg agggccgcgg tgttcagcgc
      661 gggaacggcg tcgtcgtgcc tcccgaggcg cgtcaccgag tgacccgcct tctccatgag
      721 ttcggcgagg ggcgcgccca tgtttccctt acccagaatc gcaatgttca tttggtccgt
      781 ccttgttgga aaggacgaga catagatcgc aggtcgatgg tattctcgcc gcatcatgac
      841 aacggatagg gccatacgga caaatcgcgt ggcgctcgtc gacccggacg gacatccgtc
      901 acctgtgttc gttctttccg acaaacaccc ttcgatggac ggcgactggc attcccatcg
      961 ccgcctccag ctcgtgcatg tgagcgacgg cgctgtgact gtcatcaccc ggcagggtcg
     1021 cttcgtcgtc ccccggcagc gcgctgtctg gatcggcccg cgcgtagagc accggatttc
     1081 atcacctgcc ccgttctggc tcaccacctg ctacatcgag ccgaatcttc tctctgtccc
     1141 aaaccccgcg accgtggtcg ccgtcgatcg gctcaccgat gagttgctga tcgcggcgtc
     1201 aggtctcgaa gggggtcacc cggctgtcgg gccggaggca cgcctgatcg ccgtgcttct
     1261 cgatcggctg cctggactcg aactggcgga catttttctg ccggaacccg gcgatccgaa
     1321 gctgcgacgt ctttgcagca tgctcctggc cgagccctcc cggagcgaaa atctgggcgc
     1381 gcttgcgggg ctggccggca tgaccgagcg gacagccgcg cgtctgttcg tgagcgacac
     1441 agggatgacc tttggtcagt ggcggcaatg tctgagactg cagattgcct ttgagaagct
     1501 gggcgccggc gccagtgtca cagaggccgc cttcgaaatc ggctatcggg acgtgtcatc
     1561 cttcatcacc gccttcaagg cccgttttgg cggcaccccc gccaaattcg tcaaacgctg
     1621 acgacgcgtt gaacctcggt ctgcggcgcc tgatcattct cgcgatcaaa acccgcagcg
     1681 ccgttcgaca ggccggaccg gggcagcgag gacagcccgg cgacatcttg aactccggag
     1741 agacttttct cccagaagag ggcctggccc gtcgccacgt ccgcagaata atccgtaaaa
     1801 ccaatagaac ggtagagcgc cttcgccggt tgattgccct ccagcacctc caacgtcagc
     1861 ttgcagcagt tacgttcgat cgcaagcacc tcaaccaggt gcaacatccg tcgcgccagc
     1921 cccagcccgc gatactgggg cagtacagcg acatcgtgaa cgttcaatac gggcgcacat
     1981 ttgaaggtcg agaagccctc gaaggcgttg accagcccag cggccgctcc gtcagcgaag
     2041 gccagaaccg acaaggcgtc agaccgcgcc gccagggcgc ggacaagccc ctcgcgtcag
     2101 acacgcctag cggagaactt ccacccatcg ggtctcgcgc ataggcgtcg agcaggtcca
     2161 cgatcgtcgc tgcatggcgg gggtctgcgt agtcagcacg gaggatttcg atggtcatga
     2221 gttactttcc agaggtggcg ttccacgccg gcgatgtggc ttgctctagc cgagccgtat
     2281 cccggcgcag gcgcccggat cgccgtcgcc ccggacaaaa caaccatcgt cgcgctccac
     2341 tggaggcgcc gccaagggtg atgaccgctt tccgcccatc aaagacgttc ggcaggtccg
     2401 cttctggtgc attcggtccg cgccagttcg taggcggcga aaggctcctc cgatcccgca
     2461 ctatcccttg ctatgatagt ccacgaagcc gtggtgggcg cgaccgtcca gcgattgaca
     2521 aagctggcat ccgggtaggc ataggcgact tcaaaccctc cctgatgagc ggaacccgcg
     2581 cccaacgcgg tgaaatcccc gccgaaactg tcggcgtaga agctcgaaat gttcgttgcc
     2641 ttggcgtttt cgacgggagc agtcttcccg ccgaagatca gatgggcggc gtagcgatcg
     2701 tcagcatcag ccatggcgtg gctgtccact tccaccagaa acaaggcccc gtccgtgatc
     2761 ggtttggcgt tcaaagagat cgag
//
LOCUS       DASVTM010000302         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_510081,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000302 DASVTM010000000
VERSION     DASVTM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510081"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VF633_08280"
     CDS             <1..176
                     /locus_tag="VF633_08280"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEX8471093.1"
                     /translation="WCDRGHVLTVLEGELETQLRDGRTFFLTPGMSYQVSDHGDAAHR
                     SFTAGGAKLFIVD"
     gene            237..485
                     /locus_tag="VF633_08285"
     CDS             237..485
                     /locus_tag="VF633_08285"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX8471094.1"
                     /translation="MTDTATTTAALSFEDALARLESIVSRLESGQAPLEESITLYEEG
                     ALLKAHCEARLKAAQLRVEKIVVGADGQAKGVEPAEFN"
     gene            485..850
                     /locus_tag="VF633_08290"
     CDS             485..850
                     /locus_tag="VF633_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX8471095.1"
                     /translation="MAAQPITAAADQIAFDDFMKVDIRIGRIIKAEPFPEARKPAYKL
                     TIDLGEAVGIKRSSAQITVHYTLDQLADRKVACVVNFAPRQIGPFMSEVLTLGFPDEA
                     GEVVLVGVDRDAVVGGRLF"
     gene            931..1776
                     /locus_tag="VF633_08295"
     CDS             931..1776
                     /locus_tag="VF633_08295"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX8471096.1"
                     /translation="MVTVALDRLLPRADGPESRLTEAMRYAALGPGKRLRPYFAMEAA
                     RLFDIEEQPVLRAACALECVHAYSLVHDDLPAMDNDDMRRGRPTLHIAYDEATAILAG
                     DALQTAAFDIILHEDTHEDASIRCELARRLSYASGARGMAGGQMIDLLGVRDDLGGVA
                     RMQRLKTGALFTYAFEIPLIIADAREQHQQALINFAHDIGLAYQIVDDLLDVDGDEDI
                     LGKAAGGKDAAQGKTNFVTLLGVDQARHRVAHLADQARKHLDVFGHDAENLKASVDFV
                     LNRRS"
     gene            1837..3750
                     /gene="dxs"
                     /locus_tag="VF633_08300"
     CDS             1837..3750
                     /gene="dxs"
                     /locus_tag="VF633_08300"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX8471097.1"
                     /translation="MMPETPLLDTVNIPADMRGFDIAQLKQLAEEVRAETIDAVSVTG
                     GHLGAGLGVVELTTALHHVFNTPKDILIWDVGHQCYPHKILTGRRDRIRTLRQPGGLS
                     GFTKRSESEYDPFGAAHASTSISAALGFCAARDQKGETNKVVAVIGDGSMSAGMAYEA
                     MNNAAETTGQLTVILNDNDMSIAPPVGGMSAYLAKQVSGGTYRNLREFGRKVAKHMPR
                     PFRNAARKAEEYARGMIVGGTFFEELGFYYIGPIDGHDMENLVPILKNAAAITDRPVL
                     VHVVTQKGKGYAPAESSADKLHAVVKFDVVSGKQSKSVSNAPSYTKVFGTELIKRAKL
                     DSTIVAITAAMPSGTGLDLFGQAFPDRTYDVGIAEQHAVTFAAGLAADGMKPFCAIYS
                     TFLQRGYDQVVHDVAIQSLPVRFAMDRAGLVGADGATHAGSFDVGFMGALPGMVLMAA
                     ADEADLAAMIATSLEIDDRPSAFRYPRGDGVGVEIPELSAPLEIGKGRIIREGTAVAI
                     LSLGTRLQESLKAADLLAAHGISATVADARFAKPLDADLILRLAREHEALITVEEGAM
                     GGFGAFVLQLLAEKGALDGGLKVRTLHLPDVFQDQDTPMAMYALAGLNAEQIAASALT
                     ALGVVGERGAVRA"
     gene            3839..>4253
                     /locus_tag="VF633_08305"
     CDS             3839..>4253
                     /locus_tag="VF633_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HEX8471098.1"
                     /translation="MTTTCTAFAGDRKIASGAPRDVVAAVKAATEAGQLVLIFDDADG
                     RPVEFDLRGDLDAVLARLPVEEVEEKRGRGRPKLGVTAREVTLLPRHWDWLAGQSGGA
                     SVALRKLVEGALREAEGPDRARRAKEASYRFMTAMA"
BASE COUNT          734 a         1332 c         1443 g          744 t
ORIGIN      
        1 actggtgcga ccggggccat gtcttgacgg tgctggaagg cgagctcgag acgcagttgc
       61 gggatggccg aaccttcttc ctgacgccgg gcatgagcta tcaggtctcg gaccatggag
      121 acgcggccca ccgatcgttt acagccggcg gggcgaagct gtttatcgtc gactagaggc
      181 ttcagaccga agcttcccgt tgaagcgatc ggcctccgcc tctagggtcg gtggtgatga
      241 ccgacaccgc caccaccacc gccgccctca gtttcgaaga cgccctggcg cgccttgaaa
      301 gcatcgtctc gcgtcttgag tccggccagg ctccgctcga ggagtcgatc accctgtatg
      361 aagagggcgc tctgctgaag gcgcactgcg aagcccgtct gaaggcggcg cagttgcggg
      421 tcgagaagat cgtggtcggc gccgatggtc aggccaaggg cgtcgagccg gccgagttca
      481 actgatggcc gcacaaccga tcaccgccgc cgccgatcag atcgcgttcg acgacttcat
      541 gaaggtcgat attcgcatcg gtcggatcat caaggccgag ccgttcccgg aagctcgcaa
      601 gcccgcctac aagctgacca tcgatctcgg cgaggcagtc ggcatcaagc ggtcctcggc
      661 ccagatcacc gttcattaca ccctcgatca actggctgac cgcaaggtgg cctgcgtggt
      721 gaatttcgcg ccgcgccaga tcgggccctt catgtccgaa gtcctgacgc tcggctttcc
      781 cgacgaggcc ggcgaggtcg ttctggtcgg agtcgaccgg gacgccgtgg tcggagggag
      841 gctgttctga ccaacctgcc gccgcacaag cttcatgctg ccaactcgcc ggagcaatcg
      901 gtgatcgacc gcgtctccga ggtcgccgac atggtgacgg tcgcgctcga ccggcttctg
      961 cctcgagccg acgggccgga atcgcgactg accgaggcca tgcgctatgc cgctctgggt
     1021 ccaggcaaac ggctgaggcc ttattttgcg atggaagcgg cgaggctgtt cgatatcgag
     1081 gaacagccgg tgctgcgtgc cgcttgcgcc ctcgagtgcg ttcacgccta ttcgctggtt
     1141 cacgacgact tgccggcgat ggataacgac gacatgcggc gcggtcgtcc tacgctgcat
     1201 atcgcctatg acgaggcgac tgcgatcctg gcgggtgacg ccctgcagac cgccgccttc
     1261 gacatcatcc tgcacgaaga cacccacgag gacgccagta tccggtgcga gctggcccgg
     1321 cggctgtcct atgcttccgg agcgcggggc atggcgggcg gacagatgat cgacctgctg
     1381 ggtgttcgcg acgacctcgg cggggtggcg cggatgcagc ggctgaagac cggcgccctg
     1441 ttcacctatg cgttcgagat cccgctgatc atcgcagacg cccgtgaaca gcatcagcag
     1501 gcgctgatca atttcgccca cgacatcggt ctggcctatc agatcgtcga cgacctgctc
     1561 gacgtcgacg gcgacgagga tatcctgggc aaggcggcgg gcggcaagga tgcggctcag
     1621 ggcaagacca atttcgtcac cctgctgggc gtcgatcagg cgcggcatcg ggtcgcgcat
     1681 ctggccgatc aggctcgcaa acatctggac gtgttcggtc atgacgcgga aaacctcaag
     1741 gcgagcgtgg acttcgtgct aaaccgacga tcatagggcg gagcgttcct cgctcccccg
     1801 agttactgca aagccgcttt cctcaggttc ctgcttttga tgcccgaaac cccccttctc
     1861 gacaccgtca acatccccgc cgacatgcgc gggttcgata tcgcgcagct gaagcagctg
     1921 gcggaggagg tgcgggccga gacgatcgac gcggtgtcgg tgacgggcgg ccatctgggc
     1981 gccgggctgg gcgtggtcga gttgaccacc gcgctgcacc acgtgttcaa cacgccgaaa
     2041 gacatcctga tctgggacgt cgggcaccag tgctatccgc acaagatcct gaccggtcgt
     2101 cgcgatcgga tccgcaccct gcgtcagccg ggcggcctca gcgggttcac caagcggagc
     2161 gagagcgaat acgatccgtt cggcgcggcc cacgcctcga cgtcgatctc ggcggcgctc
     2221 ggcttctgtg cggcgcggga ccagaagggc gaaaccaaca aggtcgtggc cgtgatcggc
     2281 gacggctcga tgtcggcggg catggcctat gaggctatga acaacgccgc cgagacgacc
     2341 gggcaactga ccgttatcct gaacgacaac gacatgtcga tcgcgccccc ggtcggcgga
     2401 atgagcgcct atctggccaa acaggtctcg ggcggcacct accggaatct gcgtgagttc
     2461 ggtcgcaagg tcgccaaaca tatgccgcgc ccgttccgca acgcggcccg caaggccgag
     2521 gaatatgctc gcggcatgat cgtcggcggg accttcttcg aggagctcgg cttctattac
     2581 atcgggccga tcgacggcca cgacatggag aatctggtcc cgatcctgaa gaacgcggcg
     2641 gcgatcaccg atcgtccggt gctggtccac gtcgtgaccc agaagggcaa gggttatgcg
     2701 ccggccgaga gcagcgccga caagctccat gccgtggtca agttcgacgt cgtctcgggt
     2761 aaacaatcga agtcggtctc caatgcgccg agctacacca aggtgttcgg caccgaactg
     2821 atcaagcggg ccaagctcga ctcgaccatc gtcgcgatca cggcggccat gccttcgggc
     2881 acgggtctcg acctgttcgg gcaagcattc cccgacagaa cctatgacgt cggcatcgcc
     2941 gagcagcatg cggtgacctt cgcagcgggt ctggccgcgg acgggatgaa gccgttctgc
     3001 gccatctatt cgaccttcct gcagcgcggt tacgatcagg tcgtgcatga cgtggcgatc
     3061 cagtcgttgc ccgtgcggtt cgccatggac cgggccggtc tggtcggcgc cgacggtgcg
     3121 acccacgccg gatcgttcga cgtcggcttc atgggggcgc taccgggcat ggtgctgatg
     3181 gctgcggccg acgaagccga tctggccgcc atgatcgcca cctcgctgga gatcgacgac
     3241 cgcccgagcg ccttccggta ccctcggggc gacggcgtcg gggtcgagat ccctgaactg
     3301 tccgcgccgc tcgagatcgg caagggccgg atcatccgcg aagggacggc agtcgccatc
     3361 ctttcgctgg gaacgcggct gcaggaatcg ctcaaggccg ccgatctgct ggccgcgcac
     3421 ggcatctcgg cgaccgtggc cgacgcccgt ttcgccaagc cgctggacgc cgacctgatc
     3481 ctgagactgg cccgtgagca tgaagctctg atcacggtcg aagagggtgc gatgggtggt
     3541 ttcggcgcct ttgtgctgca gttgctggct gagaagggcg cgctggacgg ggggctcaag
     3601 gtgcggacgc tgcatctgcc cgacgtgttc caggatcagg acacgccgat ggccatgtac
     3661 gcccttgcgg gtttgaacgc cgagcaaatc gccgcctcgg ccctgacagc cttgggcgtc
     3721 gtcggcgagc gcggggcagt aagggcgtaa gcgtccttct ccccttgcgg gagaaggagc
     3781 tcgccttgct tccgaaataa tatccgggta aatctctgcg cgagctggag atttcgccat
     3841 gactaccacc tgcaccgcct tcgccgggga ccggaagatc gcgtccggtg cgccgcgcga
     3901 tgtggtcgcc gccgtcaagg ctgcgaccga ggcgggtcaa ctggtgctga tcttcgatga
     3961 cgctgacggg cggccggtgg agttcgatct tcgcggcgat ctggacgcgg tgctggcgcg
     4021 gttgccggtg gaagaggtgg aagaaaaacg cgggcggggg cgacccaagc tgggtgtcac
     4081 ggctcgggag gtgacgctgt tgccgcgtca ctgggactgg ttggcgggtc agtcgggcgg
     4141 ggcgtctgtg gcgttgagaa agctggtcga gggggcgttg cgcgaggctg aggggcctga
     4201 cagggcgcgt cgggccaagg aggcgtcgta tcggttcatg accgcgatgg ccg
//
LOCUS       DASVTM010000303         8060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_666744,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000303 DASVTM010000000
VERSION     DASVTM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666744"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1474)
                     /gene="lpdA"
                     /locus_tag="VF633_08310"
     CDS             complement(53..1474)
                     /gene="lpdA"
                     /locus_tag="VF633_08310"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8471099.1"
                     /translation="MAAVDFDLIVIGSGPGGYVAAIRASQLGQKVAIVERENLGGICL
                     NWGCIPTKALLKSGEKFESLSHLKEYGLSASGATFDFNAIIQRSRGVAATMNKGVAFL
                     MKKNKIEVIEGSAKLEKGPNGGSAAPKVVIALKAGGSRTVEAKAVMLAVGARAKAIPQ
                     IGLEADGDKIWAYREALAPKKMPASIVVIGSGAIGIEFGSFYRALGAEVTVVEAVDRI
                     MPVEDEEVSKAAQKSFEKRGMKFRLGCKVTSVKKGGKGVQVAIESGGKAETLEAEVCI
                     SAVGITANTDGIGLEALGVNMDRGHITIDGHCATNVKGLYAIGDCAGAPWLAHKASHE
                     GIHAAEYIAGYKTPNVVSPIAGCTYAQPQVASVGITEQGAREAKREVKIGRFPFRVNG
                     KAVAAGEVDGFVKVLFDAKTGALVGAHMIGHEVTEMIQGYVTAIAMEATEEDIHGIVY
                     PHPTMSEAMHEAALDAYGRTIHL"
     gene            complement(1477..1956)
                     /locus_tag="VF633_08315"
     CDS             complement(1477..1956)
                     /locus_tag="VF633_08315"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX8471100.1"
                     /translation="MTSVYDFSARGIDGEDVPLERFRGQALLIVNTASKCGFTGQYAG
                     LETLHETFADKPFEVLGFPCNQFGAQEPGLAAEIASFCATSFDVQFPMFDKVEVNGPD
                     RHPLYAWLTEQKRGFLGSKAIKWNFTKFLTDREGRVVARYSPQTEPSAIQADIEKLI"
     gene            complement(1964..3295)
                     /locus_tag="VF633_08320"
     CDS             complement(1964..3295)
                     /locus_tag="VF633_08320"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEX8471101.1"
                     /translation="MMTDILMPALSPTMEEGVLAKWHVKVGDIVKAGDVIAEIETDKA
                     TMEVEAVDEGEVIEILVPEGTEGVKVNALIARLKDEGGAAAPASKATPAGEAPKGADA
                     KPAPAGHESGDAVATPEGIKSAASVLPGATPTKAPTTSGERVFSSPLARRLAAQAGLD
                     LASITGTGPHGRVVRRDVEAAGKGGAAKPAAAPASAPSPEFRKVQSLAQMGIPDGSYD
                     LIPLDGMKKAIARRMVDSIQQVPHFPLTIDVEIDALLAARAKINALLEKSGVKVSVND
                     FVIKASAMALMAVPEANASYSPEGIAMHHNADVAMAVAIDGGLITPIIRKAETKTLSQ
                     IAVESKDLAKRARDRKLKPEEFQGGTFSVSNLGMFGIKQFSSIINEPQGAIMSVGAGE
                     QRPVVKNGQLTVATVMTVTVTCDHRVVDGATGARFLQAFKPLIEDPVTMLA"
     gene            complement(3292..3609)
                     /locus_tag="VF633_08325"
     CDS             complement(3292..3609)
                     /locus_tag="VF633_08325"
                     /inference="COORDINATES: protein motif:HMM:NF028136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5076 domain-containing protein"
                     /protein_id="HEX8471102.1"
                     /translation="MSEPAEFMMTVPDSVAADPEAIEVLRMWWSKQEPVMSVKPAFGD
                     PIKFGELLAIAARHMAHGYAVRHGHDEKAAYNRILQGLSEVIKADNIGTVAEPTVSKG
                     GNA"
     gene            complement(3606..4994)
                     /locus_tag="VF633_08330"
     CDS             complement(3606..4994)
                     /locus_tag="VF633_08330"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX8471103.1"
                     /translation="MTDILMPALSPTMEEGTLTKWHIKAGDTVKAGDVIAEIETDKAT
                     MEVEAVDEGEIAEILVAEGTEGVKVNTPIARMAGEDGAAAPAPKAEPAAVSVAVAKDG
                     ADEAKSGDPEKAPAEAPVAKPKAELRDPEFPEGTKLIKTTVRDALRDAMAEEMRRDEN
                     VFLIGEEVAQYQGAYKVSRELLQEFGDRRVVDTPITEHGFAGLGVGAAMSGLRPIVEF
                     MTFNFAMQAIDHIINSAAKTLYMSGGQIRAPIVFRGPNGAASRVGAQHSQDYSAWYAQ
                     VPGLKVVAPYDAADAKGLLKSAIRDPNPVVFLEHEMMYGIEFDVPDVEDYLVPIGKAK
                     VRREGTDVTITAHARMVGFALQAAEKLAEECISCEVIDLRTLRPLDHETIVESVKKTS
                     RLVSAEEGWGPMGVGAEVVARVIEHAFDYLDAPPLRVHQEDVPLPYAANLEVLSLPGV
                     DKIVAAVKAVMA"
     gene            complement(4998..6023)
                     /gene="pdhA"
                     /locus_tag="VF633_08335"
     CDS             complement(4998..6023)
                     /gene="pdhA"
                     /locus_tag="VF633_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX8471104.1"
                     /translation="MAKAPSAKTTTKAENIPNALQASKEDLLRYYREMVLIRRFEERA
                     GQLYGMGLIGGFCHLYIGQEAVAVGVQESVRQGHDKIITGYRDHGHMLAAGMDPKEVM
                     AELTGRSGGSSRGKGGSMHMFDTATGFYGGHGIVGAQVALGTGLAFAGRYRGDDSVAF
                     IYFGDGASNQGQVYESFNMAQLWKLPAIYIIENNQYAMGTSIERSSSTTELYMRGASF
                     GIPGEQVDGMDVLAVKDAVARAVKRAREGGGPFILEVKTYRYRGHSMSDPAKYRSKEE
                     VDEVKKTRDPIDHVKMLLDAAGANEDELKAIDAEIKAIVAEAVQFAQESPEPDPSELY
                     TDVYLEA"
     gene            complement(6150..6467)
                     /locus_tag="VF633_08340"
     CDS             complement(6150..6467)
                     /locus_tag="VF633_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX8471105.1"
                     /translation="MKPYSLSAFLLLLFAYLGVQALTGERGLLSGHARDALLAEREAQ
                     LTELSARRADLEVRARYLRTDSLSRDLLEERARVVLGFSDPRDYVIRVSATPSAETIT
                     RPS"
     gene            complement(6717..7994)
                     /gene="eno"
                     /locus_tag="VF633_08345"
     CDS             complement(6717..7994)
                     /gene="eno"
                     /locus_tag="VF633_08345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX8471106.1"
                     /translation="MTDIADIVARQILDSRGNPTVEVDVTLDDGSFGRAAVPSGASTG
                     AHEAVELRDGDKTLWGGKGVQNAVDAVNGEIFDALSGMDAEDQRRIDEAMITLDGTEN
                     KGRLGANAILGVSLAVAKASAISAGLPLYRYIGGVSARVLPTPMMNIINGGMHADNPI
                     DIQEFMILPTGAESFSEALRMGSEIFHALKKQLKDAGHNTNVGDEGGFAPNLGSAEEA
                     LSFITKAGQAAGYLAGEDFHLGLDVASTEFFKNGKYEMTGEGKSFDAEGMVGYLADLC
                     ERFPIVSIEDGCAEDDFDGWKIMTERLGDRIQLVGDDLFVTNPARLAAGIGEGLANSI
                     LIKVNQIGTLSETLDAVDMANRAGYTSVMSHRSGETEDYTIADLAVATNCGQIKTGSL
                     ARSDRTAKYNQLLRIEEMLGDQGAYYGDGMLLK"
BASE COUNT         1313 a         2530 c         2655 g         1562 t
ORIGIN      
        1 gattcatgcc cgcactgagc gccacggcta cgcctaacgc aagcgctaga ccttaaaggt
       61 ggatcgtccg gccgtaggcg tcgagggcgg cttcgtgcat ggcttccgac atggtcgggt
      121 gcggatagac gatgccgtgg atgtcttcct cggtggcttc catggcgatg gcggtgacgt
      181 agccctggat catctcggtg acctcgtggc cgatcatgtg ggcgccgacg agcgcgccgg
      241 ttttcgcatc gaacagcacc ttgacgaagc cgtcgacctc gccggcggcc acggccttgc
      301 cgttcacgcg gaaggggaag cggccgatct tgacctcgcg tttcgcttcg cgggccccct
      361 gctccgtgat gccgaccgag gcgacctgag gctgggcgta ggtgcagccg gcgatcggcg
      421 agacgacgtt cggggtcttg tagccggcga tgtattcggc ggcgtggatg ccttcgtggc
      481 tggccttgtg ggcgagccag ggtgcgccgg cgcagtcgcc gatggcgtag aggcccttca
      541 cattggtggc gcagtggccg tcgatggtga tgtggccacg gtccatgttg acgcctagcg
      601 cctccagacc gatgccgtct gtgttggcgg tgatgccgac ggcagagatg cagacctcgg
      661 cctcgagcgt ctcggccttg ccgccgctct cgatggcgac ctgcacgccc ttgccgccct
      721 tcttgacgct cgtcaccttg cagccgaggc ggaacttcat gccacgcttc tcgaacgact
      781 tctgagcggc cttggagacc tcttcgtcct cgactggcat gatgcggtcg accgcttcca
      841 ctacggtgac ttctgcgccc agggctctgt agaagctgcc gaattcgatg ccgatggcgc
      901 ctgagccgat gacgacgatg gaggctggca tcttcttcgg agccagcgct tcccgatagg
      961 cccagatttt gtcgccgtca gcttcgaggc cgatttgcgg gatggccttg gcgcgggcgc
     1021 cgacggccag catgaccgcc ttggcctcga ccgtccgtga gccgccggcc ttgagcgcga
     1081 tgacgacctt gggagccgcc gacccgccat ttgggccctt ttcgagcttg gccgagccct
     1141 cgatgacctc gatcttgttc ttcttcatca ggaaggcgac gcccttgttc atcgtcgccg
     1201 cgacgccgcg cgagcgctgg atgatggcgt tgaaatcgaa cgtcgcgccg ctggcggaca
     1261 ggccgtattc cttcaggtga gacaggctct cgaacttctc gcccgacttg agcagcgcct
     1321 tggttgggat acagccccag ttcaggcaga tgccgcccag attctcgcgc tcgacgatgg
     1381 cgaccttctg acccagttgg ctcgcacgga tcgcagcgac atagccgccg gggcccgagc
     1441 cgatgacgat gaggtcgaag tctacagcag ccatgttcag atcagcttct caatatcggc
     1501 ctggatcgcc gagggctcgg tctgcgggga atagcgggcg accaccctgc cctcgcggtc
     1561 cgtcaggaat ttggtgaagt tccacttgat tgctttcgac ccgaggaacc cgcgcttctg
     1621 ctctgtcagc caggcgtaga gcgggtgccg gtcggggccg ttgacctcga ccttgtcgaa
     1681 catcgggaac tggacgtcga agctggtggc gcagaatgat gcgatttcag ccgccaatcc
     1741 gggctcctga gcgccgaact ggttgcatgg aaaacccagc acttcgaacg gcttgtcagc
     1801 aaaagtctcg tgcagcgtct caagtccggc gtactggccg gtgaagccgc atttcgacgc
     1861 cgtgttgacg atcagcaggg cctgcccccg gaaacgttcc aggggcacgt cttcgccgtc
     1921 gatcccgcgg gcgctgaaat catagaccga ggtcatgatc ggcctaggcc agcatcgtca
     1981 cgggatcttc gatcagcggc ttgaacgcct gcaggaagcg agcgccggtc gcgccgtcga
     2041 ccacgcggtg atcgcaggtg acggtgaccg tcatcacggt cgccacggtc agctggccgt
     2101 tcttgacgac cgggcgttgt tcgcctgcgc ccacgctcat gatcgcgccc tgaggctcgt
     2161 tgatgatcga agagaactgc ttgatgccga acatgccgag gttggacacc gagaaggtgc
     2221 cgccctggaa ctcttccggc ttcagcttgc gatcacgagc ccttttggcg aggtccttcg
     2281 actctaccgc gatctgcgac agggtcttgg tctcggcctt gcggatgatc ggggtgatca
     2341 ggccgccgtc gatagccacc gccatcgcca cgtcggcgtt gtggtgcatg gcgatgccct
     2401 caggcgagta gctggcattg gcttcaggca cagccatcag ggccattgcg gaagccttga
     2461 tgacgaagtc gttgaccgag accttgacgc ccgacttctc gagcagcgca ttgattttgg
     2521 cacgcgcggc cagcagggcg tcgatctcga cgtcgatggt cagcgggaaa tgcggcacct
     2581 gctggatgga atcgaccatc cggcgggcga tcgccttctt catgccgtcc agcggaatga
     2641 ggtcgtagct gccgtccggg atacccatct gggccagcga ctgcaccttg cggaactcgg
     2701 gtgaaggcgc ggaggcagga gcagcagcgg gctttgcggc gccgcccttc cctgccgctt
     2761 cgacgtcgcg acggacgacg cggccgtgtg gaccggtgcc ggtgatggac gccagatcca
     2821 gacccgcttg agcagccaaa cgacgggcca acggcgaaga gaagacgcgt tcgccagatg
     2881 ttgtgggtgc cttggtgggg gttgcgccgg gcagaacgga cgcggcggac ttgatgcctt
     2941 cgggcgtcgc gacggcgtcg ccgctctcgt gacccgcagg agccggtttg gcgtcagcgc
     3001 ccttcggggc ttcgccggcc ggagtagcct tggacgcagg agcagcggcg ccgccctcgt
     3061 ccttcaggcg ggcgatcagg gcgttgacct tcacgccctc tgtaccttcg ggcacgagga
     3121 tttcgatcac ttcgccttca tcgacggcct cgacttccat cgtggccttg tcggtttcga
     3181 tctcggcgat gacgtcaccg gccttgacga tgtcgccgac cttgacgtgc cacttggcca
     3241 gaacgccctc ttccatcgtt ggcgagaggg cgggcatcag gatgtcggtc atcaggcatt
     3301 gcctcccttg gaaacggtag gttcggcgac ggtgccgata ttgtcggcct tgatcacttc
     3361 gctgagccct tgaaggatgc ggttataggc cgccttctcg tcgtggccgt gacgcaccgc
     3421 atagccgtgg gccatgtggc gcgccgcgat ggccagcagt tcgccgaatt tgatgggatc
     3481 gccaaacgcc ggcttcaccg acatcaccgg ctcctgcttc gaccaccaca ttcgaaggac
     3541 ctcgatggcc tccggatcgg cggcgacgct gtccggcacg gtcatcatga attcggccgg
     3601 ttcgctcacg ccatcaccgc tttcacggcc gccacgatct tgtccacgcc cgggagcgac
     3661 aggacttcga ggttggcggc gtacggcaga ggcacgtctt cctggtggac gcgcagcggc
     3721 ggggcgtcga ggtagtcgaa cgcatgctcg atcacccggg ccacgacctc ggcgccgacg
     3781 cccatcgggc cccagccttc ttcggccgaa accagacggc tggtcttttt gacgctttcg
     3841 acgatggtct cgtggtccag cggacgcagg gtgcgcaggt cgatgacctc gcaactgatg
     3901 cattcctcgg ccagcttttc ggcggcctgc agggcgaaac cgaccatacg ggcgtgggcc
     3961 gtaatggtga cgtcggtacc ctcgcggcgg accttggcct tgccgatcgg gaccagataa
     4021 tcctcgacgt ccggcacatc gaactcgata ccgtacatca tctcgtgctc gaggaagacc
     4081 acggggttgg ggtcgcggat cgcggatttc agcagaccct tggcgtcggc ggcgtcatag
     4141 ggagcgacga ccttcaggcc cggaacctga gcgtaccagg cggaatagtc ctggctgtgc
     4201 tgggcgccga cgcgcgaggc ggcgccgttc ggaccgcgga acacgatagg cgcccggatc
     4261 tgaccgcccg acatatacag ggtcttggcg gccgagttga tgatgtggtc gatcgcctgc
     4321 atggcgaagt tgaacgtcat gaactcgacg atcgggcgca ggcccgacat tgccgcgccg
     4381 acgcccagac cggcgaagcc gtgctcggtg atcggggtat cgaccacgcg gcggtcaccg
     4441 aactcctgca gcagctcacg gctgaccttg taggcgccct ggtactgggc gacttcctcg
     4501 ccgatcagga agacgttctc gtcgcggcgc atttcctcgg ccatggcgtc gcgcagggcg
     4561 tcgcgtacgg tcgtcttgat cagcttcgtg ccttcgggga actcaggatc gcgcagctcc
     4621 gccttgggct tggcgacagg cgcttcggcc ggagcctttt cgggatcgcc ggatttggct
     4681 tcgtccgcgc cgtccttggc tacggccacg gagacagcag cgggctcggc cttcggcgca
     4741 ggggcggcag cgccgtcttc gccggccatg cgagcgatcg gcgtattgac cttcacgcct
     4801 tcggtacctt cggcgacgag gatttcggcg atctcgcctt catcgacggc ttcgacttcc
     4861 atcgtggcct tgtcggtttc gatctcggca atgacgtcac cggccttgac cgtgtccccc
     4921 gccttgatgt gccatttggt cagcgtgccc tcttccatgg ttggcgagag agccggcatc
     4981 agaatgtctg tcacggatca ggcctccagg tacacgtcgg tatagagctc ggacggatcg
     5041 ggttccgggc tctcttgagc gaattgaaca gcttcggcga cgatggcctt gatctcggcg
     5101 tcgatggcct tgagctcgtc ttcgttggcg ccggccgcgt cgagcagcat cttgacgtgg
     5161 tcgatcgggt cgcgggtctt cttgacctcg tcgacctctt ccttgctgcg gtatttggca
     5221 gggtcggaca tcgagtgacc gcgatagcga taggtcttca cctcgaggat gaaggggccg
     5281 ccgccttcac gggcgcgctt gaccgcacgg gccaccgcgt ccttgaccgc cagcacgtcc
     5341 atgccgtcga cctgctcgcc cgggatgccg aacgaggcgc cgcgcatgta gagctcggtc
     5401 gtggacgacg agcgctcgat gctcgtgccc atggcgtact ggttgttctc gatgatgtag
     5461 atggccggca gcttccacag ctgagccatg ttgaagctct cgtagacctg accctggttc
     5521 gaggcgccgt cgccgaaata gatgaaggcc accgagtcgt cgccgcggta gcgaccggcg
     5581 aaggccaggc ccgtgccgag cgcgacctgc gcgccgacga tgccgtgacc gccgtaaaag
     5641 ccggtggcgg tgtcgaacat gtgcatcgag ccgcccttgc cgcgcgacga accgcccgag
     5701 cgaccggtca gctcggccat gacttccttg ggatccatcc cggcggccag catgtggccg
     5761 tggtcacggt agcccgtgat gatcttgtcg tgtccctgtc tgacgctctc ctgaacgccg
     5821 acggccacgg cttcctgtcc gatgtacagg tggcagaagc ccccgatcag acccatgccg
     5881 tacaactggc ctgcgcgctc ttcgaagcgg cggatcagca ccatctcgcg gtagtagcgc
     5941 agcagatcct ctttcgaggc ctgtagcgcg ttggggatat tctcggcttt ggtggtcgtc
     6001 ttcgcggagg gggctttcgc catccagcat ctcccggctg tgccccgaag aaccacactg
     6061 gcttcagggc tacttatcgt tacggaaagc ggctttagca gccttctctc ctctaacgca
     6121 aagcgacccg gtgacgctgt aacaatagtt caggaggggc gggttatcgt ttcagccgac
     6181 ggcgtcgccg atacccggat tacataatcg cgggggtcgg aaaagccgag cacgacacgg
     6241 gctcgctctt ccagcagatc gcgtgaaaga ctgtcggtgc gcagatagcg ggcacggacc
     6301 tcgaggtcgg cgcgacgggc gctgagttcc gtcaactggg cctcgcgttc ggccagcagg
     6361 gcgtcccgag catggccgct tagcaggccg cgttcacccg tgagggcttg aacgccgaga
     6421 taggcgaaca gcagcagcag gaaggcggac agggaatacg gcttcatccg ttcgggcact
     6481 cgggacgctc cttctgagcg tggatacagt tgatggaaca tcaccgaaaa tgactaacaa
     6541 accgtagctt gaccataagt cagtaactat tgtggcgaac gcgaaacgtc ggtctttgag
     6601 tcggccatcc agacggcgcc cctgaacaaa cagtcccgcg cgtcggggcg accgacacgc
     6661 gggactgtgg aggaacgacg taagatcgac aggccgggat gcggccctgc cctctcctat
     6721 ttcagcagca tgccgtcgcc gtagtaggcg ccctggtcac ccaacatttc ctcgatgcgg
     6781 agcagctggt tgtatttggc ggtgcggtcc gagcgggcca gcgagccggt cttgatctgt
     6841 ccgcagttgg tcgcaacggc gaggtcggcg atggtgtagt cctcggtctc gcccgaacgg
     6901 tggctcatga ccgaggtgta gcccgcgcgg ttggccatat cgacggcgtc cagggtctcc
     6961 gacagggtgc cgatctggtt gaccttgatc aggatggagt tggccagacc ttcgccgatg
     7021 ccggcggcca gacgggccgg gttggtcacg aacagatcgt cgccgactag ctggatccgg
     7081 tcgccgagac gctcggtcat gatcttccag ccgtcgaaat catcctcggc gcagccgtcc
     7141 tcgatcgaga cgatcgggaa acgctcgcac aggtcggcca ggtatccgac cattccctcg
     7201 gcgtcgaagg acttgccctc gccggtcatc tcatacttgc cgttcttgaa gaactcggtc
     7261 gaggcgacgt cgaggccgag gtggaagtct tcacccgcca gatagcccgc agcctgaccg
     7321 gccttggtga tgaagctcag agcttcctcg gccgaaccga ggttaggggc gaagccgccc
     7381 tcgtcgccga cgttggtgtt gtggcccgca tccttgagct gcttcttgag cgcgtggaag
     7441 atttccgacc ccatgcgcag ggcttcggag aagctctcgg cgccggtcgg caggatcatg
     7501 aactcctgaa tgtcgatcgg attgtcggcg tgcatgccgc cgttgatgat gttcatcatc
     7561 ggggtcggca ggacgcgggc cgagacgccg ccaatgtagc gatacagcgg cagaccggcc
     7621 gagatggcgc tggccttggc caccgcgagc gagaccccga gaatggcgtt ggcgccgagg
     7681 cggcccttgt tctcagtgcc atcgagcgtg atcatcgctt cgtcgatgcg gcgctgatct
     7741 tcagcgtcca tgccggacag ggcgtcgaag atctcgccgt tgacggcgtc gaccgcgttc
     7801 tgaacgccct tgccacccca cagggtcttg tcgccgtcac gaagctcgac agcttcgtga
     7861 gcgccggtcg aggcgcccga aggcactgcg gcgcggccga agctgccgtc gtccagggtc
     7921 acgtcgacct cgacggtcgg gttgccacgg ctgtcgagga tctggcgggc gacgatgtcg
     7981 gcgatgtcgg tcatgggagc tttcgcggct gggaggaaga agcaggcgcc ggggtttagc
     8041 ccgacgcccg cgaaacgcca
//
LOCUS       DASVTM010000304         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_775703,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000304 DASVTM010000000
VERSION     DASVTM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775703"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VF633_08350"
     CDS             complement(<1..580)
                     /locus_tag="VF633_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX8471107.1"
                     /translation="MGAAFVAFLALIATPASAQPALLGHQAVLDLNSGASAWLLTATA
                     LVLMMTLPGLALFYGGMVRKKNIISVIAQSTAAFAIVSVLWFIAGYSLAFGKGPDAIN
                     GYIGGLQAAFLNGVGATTAHSLLPGIPELLFVSFQMTFAIITPALIAGAFAERMKFSA
                     SLLFFGLWHLIVYAPICHQVWGGGYLGSLGVLD"
     gene            complement(665..>5101)
                     /gene="gltB"
                     /locus_tag="VF633_08355"
     CDS             complement(665..>5101)
                     /gene="gltB"
                     /locus_tag="VF633_08355"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX8471108.1"
                     /translation="ACGVGLVAAIDGMPRREVVEYAIKSLKNVAHRGAVDPDGLSGDG
                     AGLMVETPQGFFAEQVRVIGQTLRAGPIAIGQIFLPRTDLGAQDRARAIVETEVLRAG
                     FYIYGWRQTPIDLRVVGSKADATRPEIEQIMLAAPAELAESDQSEALERELYLCRRRI
                     ENAVAGESVPGFYICSLSARSLIYKGMVRAELLDQLYPDLLDPTFEAAYAIFHQRYST
                     NTFPEWRLAQPFRMLAHNGEINTLKGNLNWMRSHEIGMTAQAFGDRDGEVKPVVQPGG
                     SDSAALDNVFEVLVRAGRTAPMAKALLIPEAWSKDAGLMKPEHGALYAYCNAVMEPWD
                     GPAAICATDGRYVVCGKDRNGLRPLRAMITHDGLLLAGSEAGLAGIPEARIARRLPIG
                     PGRMIVADLKTGVLMDESEAIDALAAAHPYQNWLDNMVDLEPVIGPGPEPRATTGEAL
                     TRRQIAAGYSREDLDMLLDPLIRDGKEALGSMGDDTPPAVLSDLPRPLAHYFRQNFSQ
                     VTNPPIDPLREAGAMSLKTRFKNLGNILAEEEAQTNVFVLDSPILTTGMYERMLSVVG
                     AGTTVTLDCSYPLPEEGDRAGAGLRAAIDRIQLEAETAVRNGSGLIVLTDQRASEERI
                     TIPMIIATAAVHLRLTDKGLRSFVSIVVRSAEALEPHGVAVLVGVGATAINPWLAQEM
                     FQERLDRGLYPGLSLRDACLNYKAGLEAGLMKTLARKGISIISAYRGGCEFEVLGLSR
                     ALTAEFFPGAPSRISGIGLAGLEQQAMRRHAAAWTEATPSPAIGGFFRIRAGQESHAH
                     DAKTIHLLQDACNRGDYKRFKQYSEAIRAQPDNAPRDLLDWRENLSPVPVYEVESVSE
                     IRKRFLTPGMSLGALSPEAHGVLNVAMNRIGARSVSGEGGEDPERYATRADGDNMNSA
                     VKQIASGRFGVTAEYLNQCREIEIKIAQGAKPGEGGQLPGFKVTEFIARMRHSTVGTT
                     LISPPPHHDIYSIEDLAQLIYDLKSINPDARVTVKLVSASGIGAIASGVAKAKADAIL
                     IAGHNGGTGASPQSSIKHAGLPWEIGLAETHQVLSLNNLRSHVVVRADGGMRTGRDIV
                     IAAILGAEEFNIGTASLIAMGCLMVRQCHSNTCPVGVCSQDPRLREKFTGTADKVVNL
                     FSFIAEEVRELLAMLGARTLDEIVGRTDLLRQVRRGGAHLDDLDLNPLLVRVEAPEKK
                     LWAQKGRAEIPETLDARVLNDAWAFLDRGQTSELSYPISNVMRTIGAGVSSAIVRRWG
                     PTGPTGVLTLKLHGSAGQSFGAFGAKGLKLELTGEANDYVGKGLSGADIVVKPIEWRE
                     NQPAIGNTTLYGATSGRLFVAGSAGERFAVRNSGAEAVVEGLGAHGCEYMTGGCVVVL
                     GPVGWNLAAGMSGGELFVLQDPAQIDLALNGDLASVAAIIPAAADRLEALVRAHAKAT
                     DSPLAKRLLANWGESVTRFARIVPTPIANAEARLDHAEAVPA"
BASE COUNT          862 a         1624 c         1782 g          833 t
ORIGIN      
        1 agtccaatac acccagcgat ccaagataac cgccgcccca gacctgatgg cagatcggcg
       61 cgtagacgat cagatgccac aggccgaaga acagcagcga cgccgagaac ttcatccgct
      121 cggcgaaggc tccggcgatc agggccgggg tgatgatggc gaaggtcatc tggaagctga
      181 cgaacaacag ctcaggaatg ccgggcagca ggctgtgcgc tgtggtcgcg ccgacgccgt
      241 tcaggaaggc ggcctgcagc ccgccgatat agccgttgat cgcgtccgga cccttgccga
      301 aggcgaggct gtagccggcg atgaaccaga gcaccgagac gatggcgaag gcggctgtcg
      361 actgggcgat gaccgagatg atgttcttct tgcggaccat gccgccgtag aacagcgcca
      421 gaccgggcaa ggtcatcatc agcaccagcg ccgtcgccgt cagcagccag gcgctggctc
      481 cggagttgag atcgaggacg gcctgatggc ccagcaacgc cggttgagcc gaggcgggcg
      541 tcgcgatgag cgccaggaag gcgacgaacg ccgcccccat caacggaaca tgagtgaggc
      601 ggcgtgacgt cagggtcatc ggcgggggag gcctatcgac aggtaaaaca ggaaggaacc
      661 cggatcatgc gggtacggcc tccgcatggt caagcctggc ctctgcattg gcgatcgggg
      721 tcggtacgat acgggcgaag cgcgtgacgc tttcgcccca gttcgccagc aaccgcttgg
      781 ccaagggcga gtctgtcgcc ttggcgtggg cgcggacgag ggcttccaga cggtcggcgg
      841 cggcggggat aatcgccgcg actgaggcga ggtcgccgtt gagcgctaga tcgatctggg
      901 ccggatcctg cagtacgaaa agctcgccgc cggacatgcc cgccgcgagg ttccagccga
      961 ccggacccag caccacgacg cagccgcccg tcatatattc gcagccgtgg gcgccgagac
     1021 cctcgacgac cgcctcggcg cccgagttcc gcaccgcgaa ccgctcaccc gccgaaccag
     1081 cgacgaacaa tcgacccgac gtcgcgccat acagggtggt gttgccgatg gcgggttggt
     1141 tctcacgcca ttcaatcggc ttgaccacga tgtcggcgcc ggacaggccc ttgccgacat
     1201 agtcgttggc ctcgccggtc agttcgagct tgagtccctt ggccccgaag gctccgaacg
     1261 actggcccgc cgagccgtgg agcttcagcg tcaggacgcc ggttggaccc gtcggacccc
     1321 agcggcgcac gatggccgag gacactcccg cgccgatggt gcgcatgacg ttggagatcg
     1381 gatagctgag ttcggatgtc tgcccccggt cgaggaaggc ccaggcgtcg ttgaggacgc
     1441 gggcgtcgag cgtctcgggg atctcggcgc ggcccttttg cgcccacagc ttcttctcgg
     1501 gagcctcgac gcgaaccagc agcgggttga ggtcgagatc gtccagatgg gcgccgccgc
     1561 gacggacctg acgcagcagg tcggtgcgtc ctacgatctc gtccagcgtc cgcgcgccca
     1621 gcatggccag cagttcgcgg acctcctcgg cgatgaagct gaacaggttg acgaccttgt
     1681 ccgccgtgcc ggtgaacttc tcgcgcagac gcgggtcctg cgaacagacg ccgacggggc
     1741 aggtgttgga atggcactga cgcaccatca gacagcccat ggcgatcagt gacgccgtgc
     1801 cgatgttgaa ctcctcggcg ccgagaatgg cggcgatgac gatgtcacga ccggtgcgca
     1861 tgccgccgtc cgcacggaca acgacgtggc tgcgcaggtt gttcaggctg agcacctgat
     1921 gggtctcggc cagaccgatt tcccatggca ggccggcatg cttgatcgac gactggggcg
     1981 aggcgcccgt gccgccgtta tggcctgcga tcaggatggc gtcggccttg gccttggcca
     2041 cgcctgaggc gatcgcgcca atgcccgagg cggagaccag cttgaccgtc acccgggcgt
     2101 ccgggttgat cgacttcagg tcgtagatga gctgtgccag atcctcgatc gaatagatgt
     2161 cgtggtgcgg cggtggcgag atcagggtcg tgccgacggt cgagtgccgc atgcgcgcga
     2221 tgaattcggt gaccttgaag ccgggcagtt gcccaccttc gccgggcttg gcgccctgtg
     2281 cgatcttgat ctcgatctcg cggcactggt tcaggtattc ggcggtgacg ccgaaccggc
     2341 ccgacgcgat ctgcttgacc gccgagttca tgttgtcgcc gtcggcgcgg gtcgcgtagc
     2401 gttccggatc ctcgccgccc tcgccggaca ccgagcgggc gccgatacgg ttcatggcga
     2461 cgttgaggac gccgtgagcc tcggggctga gcgcgcccaa tgacatgccc ggcgtcagga
     2521 agcgcttgcg gatttccgag acgctctcga cctcatagac cggtaccggc gacaggttct
     2581 cgcgccagtc gagcaggtcg cgcggcgcgt tgtcaggctg agcgcgtatg gcctcggagt
     2641 attgcttgaa gcgcttgtag tcgccgcgat tgcaggcgtc ctgcaggagg tggatcgtct
     2701 tggcgtcgtg ggcgtggctt tcctgacccg cacggatgcg gaagaagccg ccgatggccg
     2761 gcgaaggcgt cgcttcggtc caggccgcag cgtgacgacg catagcctgc tgctccagcc
     2821 cggcgagacc gatgcccgaa atccgcgatg gggcgccggg aaagaactct gcggtcaggg
     2881 cgcgggacag gcccagcacc tcgaactcgc agcccccgcg ataggcggag attatcgaga
     2941 tacccttgcg cgccagcgtc ttcatcagcc cggcctcgag accggccttg tagttcaggc
     3001 aggcgtcccg cagcgacagc cccggataga ggccgcgatc cagacgttcc tgaaacatct
     3061 cctgcgcgag ccaagggttg atggcggtgg ctccgacgcc gaccagcacg gcgacgccat
     3121 gcggctcgag cgcctccgcc gagcggacca cgatggagac gaaactgcgc aggcccttgt
     3181 cggtcaggcg cagatggacc gccgccgtgg cgatgatcat cgggatggtg atgcgctctt
     3241 ccgacgcccg ttggtcggtc aggacgatca ggccggagcc gttgcggacg gccgtttccg
     3301 cttcgagctg gatgcggtcg atagcggcgc gcagaccggc gcccgcccga tcaccctctt
     3361 caggcagcgg ataggagcag tccagcgtca cggtcgtgcc cgcgccgacc acgctcagca
     3421 tgcgctcgta catacctgtg gtcaggatcg ggctgtccag cacgaagacg ttcgtctgcg
     3481 cctcttcctc ggcgaggatg ttgccgaggt tcttgaatcg ggtcttgagg ctcatcgcgc
     3541 cggcttcgcg caaggggtcg atcggcgggt tggtgacttg tgagaaattc tggcggaaat
     3601 agtgcgccaa ggggcgaggc aggtcggaca gcaccgccgg aggcgtatcg tctcccatcg
     3661 agccgagcgc ttccttgccg tcacggatca gcggatccag cagcatgtcg agatcttcgc
     3721 ggctgtaacc ggccgcgatc tggcgacggg tcagggcctc gccggtggtg gcgcgcggct
     3781 cgggtcccgg gccgatgacc ggctccagat cgaccatgtt gtcgagccag ttctgatagg
     3841 ggtgggcggc ggccagcgca tcgatggcct cgctctcgtc catcagcacg ccggtcttga
     3901 gatcggcgac gatcatgcga ccggggccga tcggcaggcg gcgggcgatc cgggcttccg
     3961 ggatcccggc gaggcccgct tcagagcccg ccagcagcag gccgtcgtgg gtgatcatgg
     4021 cgcgcagggg acgcaggccg ttgcggtcct tgccgcagac gacatagcgg ccgtcggtgg
     4081 cgcagatggc ggctggaccg tcccatggct ccatcaccgc gttgcagtag gcgtaaagag
     4141 cgccgtgctc gggcttcatc agaccggcgt ccttggacca ggcctccggg atcagtaggg
     4201 ccttggccat gggcgcagta cgacccgcgc gaaccagaac ctcgaacacg ttgtcgagcg
     4261 cggccgagtc ggaaccgccc ggctgaacca caggcttcac ctcgccgtcg cggtcgccga
     4321 acgcctgtgc cgtcatgccg atctcgtgcg accgcatcca attcaggttg cccttcagcg
     4381 tgttgatttc gccgttgtgg gccagcatcc ggaacggctg cgccagccgc cattcgggga
     4441 aggtgttggt cgaatagcgc tggtggaaga tcgcgtaggc ggcctcgaac gtcggatcca
     4501 gcaggtcggg ataaagctgg tcgagcaact ctgcgcggac catgcccttg tagatcagcg
     4561 accgggccga cagggagcag atgtagaatc cgggcacgct ctcgccggcg acggcgttct
     4621 ctatgcgtcg gcggcagagg tagagttcgc gctcgagcgc ctcggactgg tctgactctg
     4681 ccaactcggc gggagcggcc agcatgatct gttcgatctc gggccgggtg gcgtcggcct
     4741 tggaaccgac gacgcggagg tcgatcggcg tctggcgcca gccatagata tagaagccgg
     4801 cgcgcaggac ctcggtctcg acgatggcgc gtgcgcgatc ctgagcgccc agatcggtgc
     4861 gcggcaggaa tatctggccg atggcgatgg gacccgcacg cagggtctgg ccaatgacgc
     4921 ggacctgttc ggcgaagaaa ccctgcggcg tctcgaccat caggccggcg ccgtctccag
     4981 acaggccgtc gggatcgaca gcgccgcggt gggcgacgtt cttcagcgac ttgatcgcgt
     5041 attcgacgac ttcgcggcgc ggcatgccgt cgatggcggc cacgaggccc acgccgcagg
     5101 c
//
LOCUS       DASVTM010000305        10237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_134077,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000305 DASVTM010000000
VERSION     DASVTM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134077"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2709
                     /locus_tag="VF633_08360"
     CDS             <1..2709
                     /locus_tag="VF633_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471109.1"
                     /translation="YEHIDSINSLDLDWLNQSYGLVSIDADPAAANFDNDIDTALFKN
                     LRTLSRPLWGQTTLANTQQPSALNNPLIPTGVCNTTSATGYLSANCYAVNPGKTFIYE
                     GQTARLANFGQRIGNVGLSRTFNIGGDGYNPNGATFEQRTPEQDAQRYAVGANFAILD
                     NLNLSLEAKYIDEDSFANSQPVFFDVFLSTRAGAGAGTTGARWLVNTTAYNQFELNYL
                     ENAYLPQNVKDAITANSLVNYTSPTQTAPGAPTTAAPVAAQIARHSLFGPQRGQEQNR
                     QLERYMASLNGDFDKIAFVKNINWEASYVYGKVDNYSIEDSVDVERFVLAADAVRDTA
                     GVLGTPGAIVCRSRLTLATGGALPEYFRAGDDLRDSAAGLAAVNQCQPLNIFGEGNQS
                     DAARNYVRNGSLQAVVESNEQEQAVGYIGGELWDFFGAGPIGVALGGEYRREASSASG
                     RDDQNDLFLFGLQGPDFEEVEYTSTEYFGELSLPLMRDTFLGDYAEISGSYRTFDYST
                     AGTGDVYGVNFVYRPIADIGFKASYNTSFRAPNLSENFSPLSQTFSNGFTDPCTTANI
                     RGQNDATIRANRVANCTALAVADGYAANFFDFGSATETTDDDFVQNSTSVSGVSGGNP
                     FLLPETSESKTFTVVLNPRFIPNFSLVLDYYEIEINNIISAVAAPTAAAICVNGSGLN
                     AAGCSTIFRNAPTIAGTTNVERSSGFQIGGLNAQDPGFIEGSINFAGAQTRGLDFTAN
                     YRFNTEEMLGRNFGNFFYSLRGNWLIEQKQFNNPTVPTEFTEFASNPQSPRVRLSSTL
                     SWQPVEPVRISWTVDWQTAQDIVRVRDLITGNNLDGRLPEYYDSGNFARHDLAVSYKV
                     RDDLTLRAAVTNVLDNEQPDYLGGTLYDNYDPYGRRFSIGLNFRPW"
     gene            2983..4563
                     /gene="ftsZ"
                     /locus_tag="VF633_08365"
     CDS             2983..4563
                     /gene="ftsZ"
                     /locus_tag="VF633_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8471110.1"
                     /translation="MSTLSLVRPQATELKPRIVVFGVGGAGGNAVNNMIDAGLEGVEF
                     VVANTDAQHLSFAKTDRRIQLGETITQGLGAGAHPEVGMNAAEESADEIHAHLEGAHM
                     IFITAGMGGGTGTGAAPIIAKCARDRGILTVGVVTKPFTFEGRHRMRLADAGIAELQR
                     YVDTLIVIPNQNLFRVANERTTFADAFAMADQVLHSGVRSITDLMILPGLINLDFADV
                     RAVMSEMGKAMMGTGEATGDDRALLAAQNAIANPLLDETSLKGAKAVLVNITGGLDMT
                     LLEVDEAANAISAEVDGDANIIFGAAFDPALDGKIRVSVVATGMDEAVASRIEPTAAP
                     SFNDTRRPAAPYAASRAEPVRHEPVRHEAVREPVREPIRAERAPEQAPVVPSFQSAAP
                     AVNAPVARAPEPVIHVTEERTLSPIVDPWVEEFESDRSRRPAAAEDQGDLYMDRGKAA
                     PESDYREPAHDDRDHRKSGWSLFGSKKPSRPQASYAPQQSSPQMRPASSAQPAEQQQL
                     GQVEDDLEIPSFLRRLAN"
     gene            4753..5652
                     /gene="lpxC"
                     /locus_tag="VF633_08370"
     CDS             4753..5652
                     /gene="lpxC"
                     /locus_tag="VF633_08370"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX8471111.1"
                     /translation="MPQRHDHLERTIVAPAIIAGVGVHTGERVRLAIRPAVPGTGIVF
                     VRTDITDRDNRIPVSGDAVIDARLNTMIENAAGVRLSTIEHLMAAFCALGISNVVVEC
                     DGPELPILDGSALQFVQLLDRAGFRRQATPVRYIEILEPIRISDGDKHAALLPCDRYE
                     MRFEIDFSSAVIGNQVVDFVVDEDTFRSDIMAARTFGFAHEVEALRKAGLARGGSLEN
                     AVVIDGDEILNPGGLRMEREFVKHKALDAIGDLYVLGAPLLGRYEGYKAGHAVNNMLV
                     RALLANPQSWREVTRVPELAQVG"
     gene            5765..6319
                     /locus_tag="VF633_08375"
     CDS             5765..6319
                     /locus_tag="VF633_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8471112.1"
                     /translation="MNADVSRQALNLNLRRVAGGDRSALQAVYDATSAKLFGVVLRIL
                     NDRSEAEDVLQEVYITVWTKAGQFDESRASPITWLATLARNRAIDRQRQVGRRSQRPI
                     EDAEDIPDDRESGLDAVLRSETSTRLDGCISELEEQHQKALKSAFFEGRTYDDVATLF
                     GVPTGTMKSWIRRSLIKLKACLGT"
     gene            6316..7047
                     /locus_tag="VF633_08380"
     CDS             6316..7047
                     /locus_tag="VF633_08380"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX8471113.1"
                     /translation="MTDIQPTASTPDQEAAEYALGVTTAAQRQEAERRMASDPAFAGL
                     VTAWDERLAPLIDSIPETAPPRVVWMRIAAQLGLAPRTATGGVWSNLNVWRTATGVFA
                     VAAAASMAILLVYPKPVQTPAVVQPAAPEANLTSVGLLKGETGPATFVVTLDRANKRL
                     IIAPVSSASPTGHSFEMWVLPKGQNPISLGAMDGREVVTIDTDKLIGPDGSRANLAVS
                     VEPLGGSPTGLPTGPVVASGQLQPI"
     gene            complement(7056..8225)
                     /locus_tag="VF633_08385"
     CDS             complement(7056..8225)
                     /locus_tag="VF633_08385"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX8471114.1"
                     /translation="MTRSVTGPILDRRMFATGMAALPLLGSTACATYSRTTTSTVQET
                     NWGATSDGELIQLFTLTNDAGRSVKVMTWGAAVTSVMMPDRDGALAEMVLGLDTAEDY
                     IARSRNFGATVGRYAGRIAGGKFPLDGRIVQLDTGGSANSSHGGPVGFGKRNWTGRAA
                     TSSEGPGVAMTLVSADGDQGFPGEMTITVLYRLTHDDKLTLNFTATTTKPTVLNPTHH
                     GYFNLSGDAEQGVLDHVVTIDADGFTAFDAGKFVTGEIRPVAGTPLDFRTPKTIGRDL
                     KVPDEQMTIGGGYDHNFVIRDAMGTLRRAVRIDHPGSGRSMELWTTEPGVQMYTANSV
                     EMVGRDGAAYHPYCGVAFEPQHYQNSPNRPEFPTTVLRPGQTFRSTSEYRFGFID"
     gene            8451..9866
                     /gene="uxaC"
                     /locus_tag="VF633_08390"
     CDS             8451..9866
                     /gene="uxaC"
                     /locus_tag="VF633_08390"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX8471115.1"
                     /translation="MSHPLKLNPDRLFPVDSDTRGVARRLYAEIKSLPIISPHGHTDP
                     SWFALNENFANPAELLITPDHYVFRMLHSQGMAMEDLGVPRADGGAVETDPRAIWRRF
                     AQNYHLFRGTPSRMWHDWVYAEAFGIDVRLSAETADHYYDVIDAALKTEAFKPRALFE
                     RFNIEVLTTTESPTDTLEHHKTIRASGWKGRVLTAYRPDPVVDPDYEGFRDNLKVLAE
                     QTGHDTMTWAGYLDSLRARRAFFAEMGATSTDHGHPTAFTADLSKGEAEALFARVSTG
                     PASAADAELFRGQMLTEMAAMSLEDGLVMQLHPGSFRNHSATVFNRFGRDKGCDIPTR
                     TDYVRALKPLLDRFGSDTRLTLILFTLDETSYSRELAPLAGHYPALKLGPSWWFHDSP
                     EGMRRFREQTTETAGFYNTVGFNDDTRAFLSIPARHDVARRMDCGFLAKLVLEHRMEE
                     DEAHELAHALTYGLVKAAYKL"
     gene            9863..>10237
                     /locus_tag="VF633_08395"
     CDS             9863..>10237
                     /locus_tag="VF633_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX8471116.1"
                     /translation="MTRLNQAALSSLPADVAVPAYDRVAVKTGVVHLGIGAFHRAHQA
                     VVFDDALASGDLRWGVLGASLRSPGVRDQLNPQDGLYTLVVRDGADERLRVIGAGQGV
                     LVGPEDPAALVAAMADPDVHIVT"
BASE COUNT         1831 a         3527 c         3060 g         1819 t
ORIGIN      
        1 tacgagcaca tcgactcgat caactccctg gatctcgact ggcttaacca gtcctatggt
       61 ctcgtgtcga ttgacgccga tccggccgcg gcaaacttcg acaacgatat cgacaccgct
      121 ctgttcaaga acctgcgcac cctttcgcgt ccgctgtggg gtcaaacgac tctggcgaac
      181 acgcagcagc caagcgccct gaacaacccg ctgatcccga ccggcgtctg taacacgacc
      241 tcggcgaccg gttacctgag cgccaactgc tacgccgtta accccggaaa gaccttcatc
      301 tacgagggcc aaaccgctcg ccttgccaac ttcggtcagc gcatcggcaa tgtcggcctt
      361 agccgcacgt tcaacatcgg cggagacggc tacaacccga acggcgcgac cttcgaacag
      421 cgtacgcccg agcaggacgc ccagcggtat gctgtcggcg ccaacttcgc gatcctcgac
      481 aacctcaacc tgtcgctcga agccaagtac atcgacgaag actcgttcgc gaacagccag
      541 ccggttttct ttgacgtctt cctgagcacc cgtgccggcg ccggcgccgg caccaccggc
      601 gctcgctggc tggtgaacac caccgcctac aaccagttcg agcttaacta tctcgaaaac
      661 gcttacctgc cgcagaacgt caaggacgcg atcacggcca acagcctggt caactacact
      721 tcgccaacgc agaccgcgcc aggggccccc acgaccgctg cacccgtcgc ggcccagatc
      781 gcccgccact cgctgttcgg cccgcaacgt ggtcaggagc agaaccggca gctcgagcgg
      841 tacatggcca gcctgaatgg cgatttcgac aagatcgcct tcgtcaaaaa catcaactgg
      901 gaagcttcct acgtctacgg caaggttgac aactactcga tcgaggacag cgtcgatgtc
      961 gaacgcttcg tcctggccgc cgacgccgtt cgcgacaccg cgggcgtcct gggcacgccc
     1021 ggcgccatcg tctgccgctc gcgtctgacg cttgccacgg gcggcgcgct gccagaatac
     1081 ttccgcgccg gcgacgacct ccgtgactct gccgccggtc tggctgccgt caaccagtgc
     1141 cagccgctga atatcttcgg cgaaggaaac cagagcgacg ccgctcgtaa ctacgtccgc
     1201 aacggttcgc tgcaggccgt cgtcgagtcc aatgaacagg aacaagccgt cggttatatc
     1261 ggtggcgagt tgtgggactt cttcggcgct ggtccgatcg gcgttgccct cggcggcgag
     1321 tatcgccgtg aagcctcgag cgcttcgggt cgcgacgacc agaacgacct cttcctgttc
     1381 ggtctccagg gcccggattt cgaggaagtc gaatacacga gcacggaata cttcggcgaa
     1441 ctgtcgctcc cactgatgcg cgacaccttc cttggcgatt atgcggagat cagcggttcg
     1501 tatcggacct tcgactactc caccgccggc accggtgacg tgtatggcgt taacttcgtg
     1561 tatcgtccca tcgcggacat cggcttcaag gcgagctaca acacctcgtt ccgcgccccc
     1621 aacctgtccg agaacttctc gcctctgtcc cagaccttct cgaacgggtt caccgacccc
     1681 tgcaccacgg ccaacattcg cggccagaac gacgcgacga tccgcgcaaa ccgggtggct
     1741 aactgtacgg ctctggcggt cgcagatggt tatgcggcca acttcttcga cttcggcagc
     1801 gccactgaaa ccacggacga tgacttcgtt cagaactcaa ccagcgtctc cggcgtctcg
     1861 ggcggcaacc ccttcctgct gccggaaacc tcggaatcga agaccttcac ggtcgtgctg
     1921 aacccgcgct tcatcccgaa cttcagcctg gtgctcgact actacgagat cgagatcaac
     1981 aacatcatct cggccgtggc agcgccgacc gctgccgcga tctgcgtcaa cggctcgggc
     2041 ctgaacgccg ctggttgctc gacgatcttc cggaacgctc cgaccatcgc cggcacgacc
     2101 aacgtcgagc gttcgtcggg cttccaaatc ggcggcctca acgctcagga tccaggcttc
     2161 atcgaaggct cgatcaactt cgctggcgcc cagacacgcg gtctggactt cacggccaac
     2221 taccgcttca acacagaaga aatgctgggc cgcaacttcg gcaacttctt ctactcactg
     2281 cgtggtaact ggctgatcga acagaagcag ttcaacaacc cgaccgttcc aaccgagttc
     2341 accgagttcg cttcgaaccc ccaatcaccg cgggttcgcc ttagctcgac cctttcatgg
     2401 cagccggtcg agcctgttcg gatctcgtgg accgtcgact ggcagaccgc tcaggacatc
     2461 gttcgcgtac gtgatctgat cacgggcaac aacctcgacg gccgcctgcc ggaatactat
     2521 gatagcggca acttcgcccg ccacgacctg gccgtcagct acaaggttcg cgacgacctc
     2581 acgctgcgtg ccgcagtcac caacgttctc gacaacgagc agccggacta tctcggcggt
     2641 acgctgtatg acaactacga cccgtacggt cgccgcttct cgatcggcct gaacttccgt
     2701 ccctggtaag ttcacgtcac ttgacctgaa gggaaaggcg gctcgcgaga gccgcctttt
     2761 tcgttgcgca taacttactg ggaggcggct gactgagatt tgtcccgcta acttccagcc
     2821 aaggttaacg aaatcgttcg actgacctcc gactcacgct agtcggtaac cttctcttaa
     2881 cggaatgggt gttttcctta acgtgcccgt taaccactgc gaatcggcgg ggatttcgca
     2941 gtggccgtca gggggcaggg tcggaaggtc ggaacgaaaa tcatgtctac tctctcgcta
     3001 gtgcggccac aggccactga attgaagccc cggatcgtcg tgttcggcgt cggtggcgca
     3061 ggcggcaatg ccgtcaacaa catgatcgac gccggcctcg aaggggtcga gttcgtcgtc
     3121 gccaacaccg atgcccagca tctctcgttc gccaagaccg accgccgcat ccagctgggc
     3181 gagaccatca cccaaggcct cggcgccggc gcccaccccg aagtcggcat gaacgccgcc
     3241 gaggaatccg ccgacgaaat ccacgcgcac ctcgaaggcg cgcacatgat cttcatcacc
     3301 gccggcatgg gcggcggcac cggcaccggc gctgcgccga tcatcgcgaa atgcgcgcgt
     3361 gatcgcggca tcctgactgt cggcgtcgtg accaagccgt tcaccttcga aggccgtcac
     3421 cgcatgcgtc tggccgatgc gggcatcgcc gagctgcaac gctacgtcga caccctgatc
     3481 gtcattccga accagaacct gttccgcgtc gccaacgaac gcaccacctt cgccgacgcc
     3541 ttcgccatgg ccgaccaggt gctgcactcg ggcgtccgct cgatcaccga cctgatgatc
     3601 ctgcccggcc tgatcaacct cgacttcgcc gacgtccgcg ccgtcatgtc cgaaatgggc
     3661 aaggcgatga tgggcaccgg cgaggctacc ggcgacgacc gcgccctgct ggccgcccag
     3721 aacgcaatcg ccaaccctct gctggacgag accagcctga agggcgccaa ggcggttctg
     3781 gtcaacatca ccggcggtct ggacatgacc ttgctggaag tcgacgaagc cgccaacgcc
     3841 atctcggccg aggtcgacgg cgacgccaac atcatcttcg gcgccgcgtt cgaccctgca
     3901 ctcgacggca agatccgcgt gtccgtcgtc gccaccggca tggacgaggc tgttgcctcg
     3961 cgcatcgagc cgaccgctgc tccgagcttc aacgacaccc gtcgcccggc ggcgccctat
     4021 gccgccagcc gcgccgagcc tgttcgtcat gagccagtcc gtcatgaggc tgtccgtgaa
     4081 ccggtccgcg agcccatccg tgccgaacgg gcgcccgaac aggcccccgt cgtccccagc
     4141 tttcagtcgg cggctccggc cgtaaacgcc cctgtcgccc gcgctcccga gccggtgatc
     4201 cacgtcaccg aggaacgcac gctatcgccg atcgtcgacc catgggtcga ggagttcgag
     4261 tccgaccgta gccgccgtcc ggccgccgct gaggatcagg gcgatctcta tatggatcgt
     4321 ggcaaggctg cgccggagag cgactatcgc gaaccggctc atgacgaccg cgaccaccgc
     4381 aagagcggct ggagcctgtt cggcagcaag aagccttcgc gtccacaggc gagctacgcg
     4441 cctcaacaat cctcgccgca gatgcgccct gcgtcgtccg ctcaacccgc cgagcagcag
     4501 caacttggtc aggtcgagga tgatcttgaa atcccctcgt ttctacgccg cctcgccaac
     4561 tgaaagacga gatgattaag tctgaacgcc ctggagcaat ccggggcgtt ttgcatttgg
     4621 gcgttctgtt aaccacagaa acaatccgaa actcgagttt cgtttaacgc ttgagcgacg
     4681 tcggttagac agttcagtga cccaaggcct ctccatacgt ggacgaggct tttcctgaac
     4741 gtgagaccgg ttttgccgca gcgccacgac catcttgaac ggacgatcgt cgcacccgcc
     4801 atcatcgcgg gcgtcggcgt tcacacgggc gagcgcgtgc gtctggccat ccgtccggcg
     4861 gttccgggca cgggtatcgt cttcgtccgc accgacatca ccgatcgtga caaccgtatc
     4921 ccggtctccg gcgacgccgt catcgacgcc cgcctcaaca ccatgatcga gaacgccgcc
     4981 ggcgtccggc tgtcgaccat cgagcatctg atggccgcct tctgcgccct gggcatctcg
     5041 aacgtcgtgg tcgagtgcga tggtcccgag ctgccgatcc tcgacggttc ggccctgcag
     5101 ttcgtccagt tgctggaccg cgcgggcttt cggcgtcagg cgaccccggt gcgttacatc
     5161 gagatcctcg agccgatccg tatttccgac ggcgacaagc atgcggcctt gctgccctgc
     5221 gaccgttacg agatgcgttt cgagatcgac ttctcctcgg ccgtcatcgg caaccaggtc
     5281 gtggacttcg tcgtcgacga ggacaccttc cgttcggaca tcatggccgc ccgtacattc
     5341 ggtttcgccc atgaggtcga ggccctgcgc aaggctggtc tggcgcgcgg cggctcgctc
     5401 gagaacgcgg tcgtcattga cggcgacgag atactgaacc ccggcggcct gcggatggaa
     5461 cgtgagttcg tgaagcacaa ggcgctcgac gccatcggcg acctgtacgt cctcggcgcg
     5521 ccgctgctgg gccgctacga aggctacaag gccggtcacg ccgtcaacaa catgctggtc
     5581 cgtgccctgc tggccaaccc ccagagctgg cgcgaagtca cccgcgtgcc ggagctggcc
     5641 caggtcggct gatccgggct ctccgctctt gatggatgcg tccgcttctc ccggcgccac
     5701 gccttgcggc gaaagctgtg tcgtgcgacc ggtgcagcgt cttgaataag cacaaaagcg
     5761 acgcatgaac gccgacgtta gccgacaggc cctcaacctg aatctccggc gtgtcgcggg
     5821 gggcgaccgc tcggcgttgc aggccgtgta tgacgctaca tccgcgaaac tattcggagt
     5881 ggtgcttcgt atcttgaatg atcgaagcga ggcagaagac gtgctgcagg aagtctacat
     5941 cacagtctgg accaaggcgg gacagttcga tgaaagtcga gccagcccga tcacctggct
     6001 ggcgactttg gcccgcaatc gcgcgattga ccgtcagcgt caggtcggcc gtcgtagtca
     6061 gcgtcccata gaggacgccg aggatatccc cgacgacagg gagtccggtc tggacgcggt
     6121 cctgcgttcc gaaacctcga cccggctcga tggctgcatc tccgagcttg aggaacagca
     6181 ccagaaggcc ctcaagtccg ccttcttcga aggccgcacc tatgacgatg tggcgaccct
     6241 tttcggcgtg cctacgggca cgatgaagag ctggatccgt cgctccctga taaagctgaa
     6301 ggcctgcctc ggcacatgac cgacattcaa ccgaccgcat cgacaccgga tcaggaagcc
     6361 gccgagtacg ccttgggcgt gaccactgcc gcccagcgtc aggaggccga gcgccgcatg
     6421 gcctctgatc cggcttttgc cggtctggtc accgcctggg acgagcgcct cgcaccgctg
     6481 atcgactcaa ttcctgaaac ggccccgccg cgcgtcgtgt ggatgcggat tgcggctcag
     6541 ttgggtctgg ctccgaggac ggcgacgggc ggcgtctgga gcaacctcaa cgtctggcgc
     6601 accgcgaccg gcgtcttcgc agtggccgcc gccgcgtcga tggctatcct gctggtctat
     6661 ccaaagccag tccagacgcc ggcggtcgtg cagccggcgg ctccggaagc gaacctgacc
     6721 tctgtcggtc tgctgaaggg cgagaccggt ccggcgacct tcgtcgtcac gctcgacagg
     6781 gccaacaagc gactgatcat cgcgcccgtc agctcggcca gcccgaccgg acactcgttc
     6841 gagatgtggg tcctgccgaa gggccagaac ccgatctcgc tcggcgcgat ggacggcagg
     6901 gaggtcgtca ctatcgacac cgacaagctg atcggtcctg acggatcgcg cgccaacctg
     6961 gccgtctcgg tcgagcccct cggcggttcg cccaccggcc tgccgaccgg ccccgtcgtc
     7021 gccagcggac aactgcagcc gatttaagcc agcgtctagt cgataaaccc gaaccggtat
     7081 tcgctcgtcg accggaacgt ctggccgggc ctgaggaccg tcgtcggaaa ctccggtcgg
     7141 ttggggctgt tctgatagtg ctgcggctcg aaggcgacgc cgcagtaggg gtggtaggca
     7201 gccccatcgc gcccgaccat ctccaccgaa ttggcggtgt acatctgcac ccccggttcg
     7261 gtcgtccaca gctccatgct ccggccgctg ccgggatgat cgatccgcac ggccctgcgc
     7321 agcgttccca tcgcgtcccg gattacgaaa ttatggtcat agccaccgcc gatggtcatc
     7381 tgttcgtcgg gaaccttcag atcgcgccct atggtcttgg gcgtgcggaa gtcgagcggc
     7441 gtgcctgcga ccggacggat ttcgccggtc acaaacttgc cggcgtcgaa cgcggtgaag
     7501 ccgtcagcgt cgatcgtcac gacgtgatcc agcactccct gttccgcgtc gccgctgagg
     7561 ttgaaatagc cgtggtgcgt cgggttcagc accgttggct tcgtcgtcgt tgcggtgaag
     7621 ttgagcgtca gcttgtcgtc atgcgtcagg cggtagagga cggtgatggt catctcgccg
     7681 ggaaagcctt gatcgccatc cgcgctgacc agtgtcatgg ccacgccggg gccttcggac
     7741 gacgtggcgg cgcgaccggt ccagttgcgt ttgccgaacc cgaccggtcc accgtgcgag
     7801 gagttggcgc tgccgccggt gtcgagctgg acgatccggc catcgagcgg gaatttccca
     7861 cccgcgatcc ggcctgcata tcggccgacg gtcgcgccga aattgcgtga gcgggcgatg
     7921 tagtcctcgg cggtgtcgag ccccaacacc atctcggcca aggccccgtc acggtccggc
     7981 atcatgaccg aagtcaccgc cgcaccccac gtcatgacct ttaccgatcg gcctgcatcg
     8041 ttggtcaggg tgaagagctg gatgagttcg ccgtctgacg tggcgcccca gttcgtctcc
     8101 tgaacagtgg atgtcgtcgt ccggctgtag gtcgcgcagg cagtggagcc gagcagcggc
     8161 aaggccgcca tcccggtggc aaacatccgg cggtcaagga tgggtccggt cacggatctg
     8221 gtcatgctcg tcttcctgtt tccctgcggc ctccggaatc tggcgttccg acgaacctga
     8281 gtgcaacatg gctgcactga tccactatgc gcatttgcca aaggaatgtc accggtgtca
     8341 tagacgccgg gccctcgaca tttagatcgc cgccagacgc gatccgtcgt catcacgatc
     8401 acccaggaag cgctcgccac ggtccggcgg cgcgcagtag agtcgcaccg atgagccatc
     8461 ctctgaagct gaatcccgac cgtcttttcc cggtcgactc cgacactcgt ggggtggccc
     8521 ggcgcctgta cgccgagatc aagagcctgc cgatcatcag cccgcacggc cacacggacc
     8581 cgtcgtggtt tgcgctgaac gagaatttcg ctaacccggc cgagctgctg atcacgcccg
     8641 accactacgt cttccgcatg ctgcactcgc agggaatggc gatggaggat ctgggcgtgc
     8701 cgcgtgctga cggcggtgcg gtcgagaccg atccgcgggc gatctggcgc cggttcgccc
     8761 aaaactatca cctgttccgc ggcacgcctt cgcgcatgtg gcacgactgg gtctatgccg
     8821 aggcgttcgg catcgacgtg cgcctctcgg ccgagacggc ggaccactat tacgacgtga
     8881 tcgacgccgc gctgaagacc gaggccttca agccgcgcgc cctgttcgag cgcttcaaca
     8941 tcgaggtcct gaccacgacc gagtcgccga ccgacacgct ggagcaccac aagaccattc
     9001 gcgcctcggg ctggaagggc cgcgtcctga ccgcctatcg ccccgacccc gtggtcgacc
     9061 ccgactacga aggcttccgc gacaatctga aggtgctggc cgaacagacc ggccatgaca
     9121 ccatgacctg ggccggttac ctcgatagct tgcgcgcacg ccgggcgttc ttcgcggaga
     9181 tgggcgcgac ctcgaccgac cacggccacc ccaccgcctt caccgccgac ctgtcgaagg
     9241 gcgaggccga agccctgttc gcccgcgtct cgaccggccc cgcatccgcc gccgacgccg
     9301 agctgttccg gggccagatg ctgaccgaga tggccgcgat gtcgctcgag gatggtctgg
     9361 tgatgcagct tcatcccggc tcgttccgca accactcggc cacggtgttc aaccgcttcg
     9421 gccgcgacaa gggctgcgat atcccgaccc gcaccgacta tgtccgcgcg ctgaaacccc
     9481 tgcttgaccg gttcggctct gacacccgtc tgaccctgat cctgttcacc ctggacgaga
     9541 cctcctacag ccgcgaactg gcgccgctgg cgggtcacta cccggcgctg aagctgggcc
     9601 cgagctggtg gttccacgac agccccgagg gcatgcgccg cttccgcgag cagaccaccg
     9661 agacggcggg cttctacaac accgtcggct tcaacgacga cacccgggcg ttcctgtcga
     9721 tcccggcgcg tcacgatgtt gcgcggcgca tggactgcgg cttcctcgcc aaactggtgc
     9781 tcgaacaccg gatggaagag gacgaggccc acgaactggc ccacgccctg acctatggac
     9841 tggtcaaggc ggcctacaag ctgtgacccg cctgaaccaa gccgcgctgt cgagcctgcc
     9901 tgccgatgtg gccgtgcccg cctacgaccg ggtggcggtt aagaccggcg tcgtccacct
     9961 cggcatcggc gcctttcacc gggcgcacca ggcggtggtg ttcgacgatg ctctggcgtc
    10021 cggagacctg cgctggggcg tgctcggcgc ctcgctgcgt tcgccgggcg ttcgcgatca
    10081 actgaacccg caggacggcc tgtatacgct ggtggtgcgt gacggcgccg acgagcgtct
    10141 gcgcgtcatc ggggccgggc agggcgttct ggtcggaccg gaggaccctg ccgcgctggt
    10201 cgcggccatg gccgatccgg atgtccacat cgtcacc
//
LOCUS       DASVTM010000306         7700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_243230,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000306 DASVTM010000000
VERSION     DASVTM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243230"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF633_08400"
     CDS             complement(<1..215)
                     /locus_tag="VF633_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471117.1"
                     /translation="MVNHLKNSLNWDAFRHRTLAPAGARAGQWRDWVVANDPVYRSFR
                     RPGRTEKIVGGIALLLVAIIVIFLLLF"
     gene            430..741
                     /locus_tag="VF633_08405"
     CDS             430..741
                     /locus_tag="VF633_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471118.1"
                     /translation="MTKTALIACALFAATAAPAFAGEAPRTAPPAAQSSARVLVTVCE
                     RDALTQAAFRQNYGARPVYVTAEQVLNARAAGERWSAPRCMTTSGHQRLTQALNSRTG
                     S"
     gene            complement(810..2903)
                     /locus_tag="VF633_08410"
     CDS             complement(810..2903)
                     /locus_tag="VF633_08410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8471119.1"
                     /translation="MRYRSLLSGACAALALMTASAVLAQTTAAPAAAQAAGNAFTYTD
                     MISLNRLGDPQVSADGRWLVYSVTATDVSANKRTGSLWLKDLQGDHEATRLAISDDGA
                     NTARWGSDGRLYFLSAKSGTTQVWSAAADGTGATQVTSLPLDVNAYKLSSDSSKIAVS
                     LAVFGDCADLQCSVDRMKTNAEKKSTGQVYDRMFVRHWDTWNDGTQNHLFVVPIAGGD
                     AVWVTKGFDGDNPSKPFGDESEFTFTPDNSAIVFSARLAGKSEPWSTNFDLYQTNGLT
                     TGDGQFTNLTEDNPAWDTGAVFSPDGKTLAYRAMARPGFEADKYSIFLRDVATGQTRQ
                     IAADWDRSADGLQWSKDGSTLYTTAGDVGQSRLFSINVRTGSVVPITGEGHVSAFVQT
                     PSGFIFAKDSLTEPSDLYAKTFRGREMPRRLTEVNPSLDAKVFGEPEQFTFAGWNGEE
                     VHGYVIKPAGYVEGQKYPVAFLIHGGPQGSFGNSWSYRWNPETYAGAGYAVVMIDFHG
                     STGYGQGFTDAISQHWGDRPFEDLQKGWAAAQQKYAYLDGSNACALGASYGGYMINWI
                     AGNWPNAFKCLVNHDGVFDTFGMGYSSEELWFTEWEYGGTPYENPRGYQEFNPANHVD
                     KWKTPMLVVQGDRDFRIPTAQGLSTFTALQRRGIDSRLVVFPNENHWVLKPANSVQWH
                     NEVFGWLDKYLKPAS"
     gene            3035..3889
                     /locus_tag="VF633_08415"
     CDS             3035..3889
                     /locus_tag="VF633_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX8471120.1"
                     /translation="MNSSVPDIAAPHICDVLIAERAPRLTGSWAWPVVRPALYKLLNY
                     KAARKMADGVAGLSGQGAFDYVSDLLDLKVSTLNLDRIPATGRCVIVCNHPTGVADGV
                     AVYDAIKKRRSDVIFFANADALRVSPRLNEIVIPVEWVVEKRTREKTRATLDAARAAF
                     EGERCVVMFPAGRLARVGKDGSLTDPEWASTAASMARKYGAPVVPMHVAGPTSRLFHL
                     FNRVSDELRDITLFHELLNKRKKAFQLKVGLPIPPTRLDIDAVKATYALKAFTERVLP
                     SQPDAVFA"
     gene            3886..4371
                     /gene="tsaE"
                     /locus_tag="VF633_08420"
     CDS             3886..4371
                     /gene="tsaE"
                     /locus_tag="VF633_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX8471121.1"
                     /translation="MSEPHTIALADAEATTALGARIAPLLQPGDAVLLYGPLGMGKST
                     LARGLIRALTRPDEDVPSPTFTLVQFYEATPPVAHFDLYRLTRADEAFEIGLDEALDD
                     GVALVEWPERLGEDVSWLVGGNRLCITLSEDGPDPETAGRVATILAAGSWETRIDHVF
                     A"
     gene            4358..5452
                     /locus_tag="VF633_08425"
     CDS             4358..5452
                     /locus_tag="VF633_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8471122.1"
                     /translation="MSSPDRESQRIDFLKAAGLGDATRAPLPGDASTRRYERLTTTDG
                     ISLMLMDQVAAAESPPADPSWTPEQRHASGWNAIARLSAGRVEAFAAVAEHLRSVGLS
                     APAMPALDAPAGLAVLEDFGDGLFAHVIADGEPEAPLYLAAIEALVQLHDAETPETLT
                     GPAGDWPLQVYDATALQGGADLFVEWMPKLDTSLLFDAEALADWNAAWAPITAEGEAG
                     ASVMAHRDYHAENLIWLPAREGAARVGMIDFQDAVRAHPSWDLHSLLQDARRDVSPAL
                     EAEALDHYFSLRPSVDRVAFMRSYAGLAALNEARILGIFARLIVRDGKPKYAAFMPRV
                     WAHLNANLKRPGLEQVAVWFDRYVPVERRK"
     gene            5449..6198
                     /locus_tag="VF633_08430"
     CDS             5449..6198
                     /locus_tag="VF633_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8471123.1"
                     /translation="MTVPKTAMVLAAGLGTRMRPLTDDRPKALVEVSGKALIDHMLAR
                     LAEAGVERAVVNVHAFADRLIGHLEAYEGPLEIIISDERQHALPLETGGGIKAAAAHL
                     GEAPILVANIDSVWIEDREPPAQAIRELCDGFDPDQMSCRLMLAHMARTTGFDGPGDF
                     LLAEDGRLTARRTAGVPSAPLNYMGVHIVEPRPIYARPETEFGLFPAGGGLWGGWEAE
                     GRLHGLVMEGDWMHVGDPAARDVAELRLAGA"
     gene            6198..>7700
                     /locus_tag="VF633_08435"
     CDS             6198..>7700
                     /locus_tag="VF633_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEX8471124.1"
                     /translation="MTDRFDPFAGAGPRWLNIPSHRPFLEDLAAGVLDWLGDAAPETL
                     SDAVILLPNRRAARAFGAALSRLSGDKPILLPQVRPLGDLEEDEPPFAPGEIGLDLPP
                     AIAPLTRRFEMARMIAEDLKDEFEDHLSPLRALELADAFGGFLDSCQIEEIGDLQRVE
                     TMVEADLAEHWRTSAKFLGLAVQAWPDRLEAMGLMDPAERRAKLLRLLALRWDEAPPT
                     GPVIAAGSTGSAPAAAAVLKAVAKAPQGCVVVPGLDMDLDPAVWATLGDDEQHPQNAL
                     WRLLDHGEVAREAVRPWFQPRVEPVVQARGLARQRLINEALRPAGATSDWRKEIARIR
                     EVAAPVQGADPIALGLENLSVVTLRTEEDAAATLALMMRETLEQRHPDGRPKTCALVT
                     PDLDLGRRVAARLARWDIIPDSSSGAPLSRMPAGVLIDLAARWIADPLKPQTILALLK
                     HPLVRIDLGEADVFAAIRAFEKHALRGPRGRRWTDLHRRLNDAAEPREGQT"
BASE COUNT         1255 a         2464 c         2594 g         1387 t
ORIGIN      
        1 tcgaacagca gcaggaagat aacgatgatc gcgaccagca ggagcgcgat gccgccgacg
       61 attttttctg tccgcccagg acggcggaaa gaccgataga ccgggtcatt ggccaccacc
      121 cagtcgcgcc attgaccggc acgcgcgcca gcgggagcga gcgtccggtg gcggaaggcg
      181 tcccagttca agctgttttt caagtggttg accaatgttc aatcccggac gaaccgtttc
      241 aacgcacgac ggcgtgactt ggcgccatca gttgctcgct tctgtagcgt gcacacagga
      301 aaaccatccg tcagaagatg gaatgtcgta cttcggtcgc gtcgctgtca catcatcttt
      361 acaaacgccg agaagcgcgc attattcgtc ctgactctga cgtttttcag ggattgggag
      421 gactacccta tgaccaaaac cgccttgatc gcctgcgccc tgttcgcggc gactgctgcc
      481 cctgcattcg ccggtgaggc gccgcgcacg gccccgcccg ccgcgcaatc gtcggcccga
      541 gtgctcgtca cggtgtgtga acgcgacgcg ctgacccagg ccgccttccg ccagaactat
      601 ggtgcgcgcc cggtctacgt caccgccgaa caggtgctga acgcccgcgc tgctggcgag
      661 cgctggtcgg ccccgcgctg catgaccacc agcggacatc agcgcctgac gcaagctttg
      721 aacagccgca ccggctcctg agctcggctg ctgcaaaaag aagggcgctg cctcgcggca
      781 gcgccctttt tcgtgcgtgg tcgtcggcct cagctagcgg gcttgaggta tttgtcgagc
      841 cagccgaaca cctcgttgtg ccactgcacg ctgttggcgg gcttgagcac ccaatggttc
      901 tcgttcggga acacgaccag acggctatcg atgccgcgac gctgaagcgc agtgaaggtc
      961 gacaggccct gagcggtcgg gatgcggaag tcgcggtcgc cctgaaccac cagcatcggc
     1021 gtcttccact tgtcgacgtg gttggccggg ttgaattcct gatagccgcg cgggttttca
     1081 tagggcgtgc cgccgtattc ccactcggtg aaccacagct cctcggacga atagcccatg
     1141 ccgaaggtgt cgaagacgcc gtcgtggttg accaggcatt tgaacgcgtt tggccagtta
     1201 ccggcgatcc agttgatcat atagccgccg tacgaagcgc cgagtgcgca ggcgttggag
     1261 ccgtcgaggt aggcgtattt ctgctgcgct gcggcccagc ccttttgcag atcctcgaac
     1321 ggccggtcgc cccagtgctg cgagatcgca tcggtgaagc cctggccgta gccggtcgag
     1381 ccgtggaaat cgatcatcac gacggcatag cccgcacccg cataggtctc ggggttccag
     1441 cgataagacc agctgttgcc gaacgaaccc tgcggtccgc catggatcag gaaggcgacc
     1501 gggtatttct ggccctcgac atacccggcg ggcttgatga catagccgtg gacttcttca
     1561 ccgttccagc cggcaaaggt gaactgctcc ggctcaccga acaccttggc gtccagcgac
     1621 gggttcacct cggtcaggcg gcgcggcatt tcgcgacccc ggaaggtctt ggcgtagaga
     1681 tcgctcggtt cggtcaggct gtcctttgcg aagatgaagc ccgacggggt ctgcacgaag
     1741 gccgagacgt ggccttcacc ggtaatcggc acgactgatc cggtgcggac gttgatcgag
     1801 aacagccggc tctgaccgac gtctccggcg gtggtgtaga gtgtcgagcc gtccttcgac
     1861 cattgcagac catcggccga gcgatcccag tcagccgcga tctgacgcgt ctgaccggtc
     1921 gcgacgtcac gcaggaagat tgaatattta tcggcctcga agcccgggcg agccatggcg
     1981 cgataagcga gcgtcttgcc gtccggcgag aacaccgcgc ctgtatccca agcggggttg
     2041 tcctcggtca ggttggtgaa ttggccgtcg ccggtggtca gaccgttggt ctggtacaga
     2101 tcgaaattgg tgctccacgg ctcgcttttg ccagcgagac gagccgagaa cacgatggcc
     2161 gaattgtctg gcgtgaaggt gaactcgctc tcgtcgccga agggcttgga gggattgtcg
     2221 ccgtcgaagc ccttggtcac ccagacggcg tcgccgcctg caatcgggac gacgaacaga
     2281 tgattctgcg tgccgtcgtt ccaggtgtcc cagtgacgca cgaacatgcg gtcatagacc
     2341 tgaccggtcg atttcttctc ggcgttggtc ttcatccgat cgaccgagca ttgcaggtcg
     2401 gcgcagtcac cgaacacggc cagcgacacg gcgatcttgg acgagtcgga gctcagcttg
     2461 taggcgttga cgtcgagcgg caggctcgtg acctgggtcg cgcctgtgcc gtcggcggcg
     2521 gcgctccaga cctgggtcgt gcccgatttg gccgacagga aatacagacg accgtccgag
     2581 ccccagcgcg cggtgttggc gccgtcgtcg gaaatggcga gacgggtcgc ctcgtggtcg
     2641 ccctgcaaat ccttgagcca caggctgccc gtccgcttgt tagcggagac gtcggtggcc
     2701 gtcaccgaat agaccagcca gcgcccgtca gccgagacct gcggatcgcc aagccggttc
     2761 aggctgatca tgtcggtgta ggtgaaggcg ttcccggcgg cttgtgccgc agcaggagcg
     2821 gcggtcgttt gcgccagcac ggccgaagcc gtcatcaggg cgagggcggc gcaggcgccg
     2881 gagagcaggg agcgataacg catctagcgg tccttcagat ctggaggcgg caggcgtagc
     2941 accgtcgtct gtccggcgaa agttgcggtt atggtggaac gtcggacatc ggcgcgcttg
     3001 ctccacggcc ccgatgcggc gacaaccggc gcgcatgaat tcctctgtcc ccgacatcgc
     3061 tgctccgcat atctgcgacg tgctgatcgc cgagcgtgca ccgcgtctga caggctcatg
     3121 ggcgtggccg gtggtgcgtc cggcgttgta caagctgctc aactacaagg cggcgcgaaa
     3181 gatggcagac ggcgtcgcgg ggctctcggg gcagggagcg ttcgactatg tgtcggatct
     3241 gctggacctg aaggtctcga cgctgaacct cgaccgcatt ccggcgaccg ggcgttgcgt
     3301 catcgtctgc aaccacccga ccggggtcgc cgacggggtc gccgtctacg atgcgatcaa
     3361 gaagagacga tcggacgtca tcttcttcgc taatgccgat gcactccggg tctcgccccg
     3421 gctgaacgag atcgtcatcc cggtcgaatg ggtggtcgaa aagcgcacgc gtgagaagac
     3481 ccgcgccacg ctggacgccg ctcgcgccgc cttcgaaggc gagcgttgcg tggtcatgtt
     3541 ccccgccgga cggttggctc gcgttggtaa ggacggctcg ttgactgacc ccgaatgggc
     3601 ctcgacggcc gcctcgatgg cgaggaaata cggagcgccg gtggtgccga tgcatgtcgc
     3661 tggcccgacc tcgcgcctgt tccacctgtt caatcgagtg tcggacgaac tgcgcgacat
     3721 caccctgttt catgagctgc tcaacaagcg gaagaaggcg ttccagctca aggtcggtct
     3781 gccgatcccg ccgacccggc tcgacatcga tgcggtaaag gcgacgtacg cgctgaaggc
     3841 cttcaccgag cgggttctgc ctagccagcc ggacgcggtg ttcgcatgag tgagccccac
     3901 acgatcgcct tggccgacgc cgaggccacg acggcgcttg gggcacggat cgcgccgctg
     3961 ctgcagccgg gcgatgcggt gctgctttac ggtccgctcg gcatgggcaa gtcgacgctg
     4021 gcgcgcgggt tgatccgggc gctgacacga cccgacgaag acgtgccgtc gccgaccttc
     4081 accctggttc agttctacga ggcgactccg cccgttgcgc attttgatct ctatcggctg
     4141 acccgagccg acgaggcgtt cgaaatcggg ctggacgagg cgctggatga cggcgtcgca
     4201 ctggtcgagt ggcccgaacg gctgggcgaa gacgtcagct ggctggtcgg cggaaaccgc
     4261 ctgtgcatca ccctgtccga ggacggaccc gatcctgaaa ccgccggtcg tgttgcgacc
     4321 attcttgccg caggatcgtg ggaaacgaga atcgaccatg tcttcgcctg atcgtgaatc
     4381 ccagcgtatc gacttcctca aggccgccgg ccttggcgac gctacgcgtg cgcctctgcc
     4441 gggcgacgcc tcgacgcggc ggtacgaacg gctaacgacg acggacggaa tttcgttgat
     4501 gctgatggat caggtcgcgg ctgccgagag cccaccggcc gatccgtcgt ggaccccgga
     4561 acaacgccac gccagcggct ggaacgcaat cgcccgtctg tcggccggac gggtcgaggc
     4621 cttcgcggcg gtggccgagc atctgcgctc ggtcggactg tcggcgcccg ccatgcctgc
     4681 gctcgatgcg cctgccgggc tggcggtgct ggaagatttc ggcgacggtc tgttcgccca
     4741 tgtgatcgcc gacggtgaac cggaggctcc gctctacttg gccgcgatcg aagcgctcgt
     4801 ccaactgcac gacgccgaga cgcccgagac cttgactggc cctgcgggcg attggccgtt
     4861 gcaggtctac gacgcgacag cgctgcaagg tggcgcggac ctgttcgtcg aatggatgcc
     4921 taagctcgac accagcctgt tgttcgacgc cgaggcgctt gcggactgga acgctgcgtg
     4981 ggcgccgatc acagctgaag gcgaggcagg tgcgtcagtg atggcgcacc gcgattacca
     5041 tgccgagaac ctgatctggc tgccggcccg cgagggcgcc gcccgcgtcg gtatgatcga
     5101 ctttcaggac gcggtgcgtg cgcatccgtc ctgggacctg cattcgttgc ttcaggacgc
     5161 gcgtcgcgac gtgtcccctg cgctcgaggc cgaggcgctg gaccactatt tctcactgcg
     5221 cccgtcggtc gatcgcgtcg ccttcatgcg gtcctacgct ggtctcgctg cgctcaatga
     5281 ggcgcgtatc ctcggcatct tcgcgcggtt gatcgtgcgt gacggcaagc cgaaatacgc
     5341 cgccttcatg ccgcgcgtat gggcgcatct gaacgccaac ctgaaacggc ccgggctgga
     5401 gcaggtcgcc gtctggttcg accgttacgt gcccgtggag cgccgcaaat gaccgttcca
     5461 aagacggcga tggtgctggc cgcagggctc ggcacccgga tgcggcccct gaccgacgac
     5521 cggcccaagg ccctcgtcga ggtcagcggc aaggccctga tcgatcacat gctggcccgg
     5581 ttggccgagg ccggcgttga gcgggcggtg gtcaatgtcc atgcgttcgc cgatcggttg
     5641 attggccatc tggaagcgta tgaagggccg ctggagatca ttatttccga tgagcgccag
     5701 catgctttgc cgctcgaaac cggcggcggc atcaaggctg cggccgccca tctgggcgaa
     5761 gcgccgatcc tcgtcgccaa tatcgacagc gtctggatcg aggaccgcga gccgcctgcg
     5821 caggcgatcc gtgaactgtg cgacggcttc gatccggatc agatgagctg ccgcctgatg
     5881 ctggcgcata tggcgcgcac gaccggtttc gatggtcccg gtgacttcct gctggccgaa
     5941 gacggccggc tgaccgcgcg tcggacggct ggcgtaccga gcgcgccgct caactatatg
     6001 ggcgtgcaca tcgtcgaacc gcgcccgatc tatgcccggc ccgaaaccga gttcggcctg
     6061 ttccctgcgg gtggcggcct gtggggaggc tgggaggcgg agggtcgcct gcacggcctg
     6121 gtcatggaag gcgactggat gcatgtcggt gatcccgctg cgcgcgatgt cgccgaactg
     6181 cgtctggctg gagcctgatg acggatcgtt tcgatccttt tgctggtgcc ggtccgcgct
     6241 ggctgaacat tccctcgcac cggccattcc tcgaggatct ggccgccggc gtgctggact
     6301 ggctggggga tgctgcgccc gagacgctga gcgacgccgt catcctgttg cccaaccggc
     6361 gtgcggcgcg ggcgttcggc gcggccctgt cgcggctgag tggcgacaag ccgattcttc
     6421 tgccgcaggt ccggccgctc ggtgatctgg aagaagacga gcctccgttt gcgcccggcg
     6481 agatcggtct cgacctgccc cccgccatcg cgcccctgac gcgccggttc gagatggcgc
     6541 ggatgatcgc ggaggatctg aaggacgagt tcgaagacca tctgtcgccg ttgcgcgcgc
     6601 tggagctggc tgacgctttc ggcggcttcc tcgactcttg ccagatcgag gagatcggcg
     6661 acctgcagcg ggtcgagacg atggtcgagg ccgatctagc cgaacactgg cggacgtcag
     6721 cgaaattcct tggattggcg gtgcaggcgt ggcccgaccg tctggaggcc atggggctga
     6781 tggatccggc ggagcggcgg gcgaaactgc tgcggctgct ggctttgcgt tgggatgagg
     6841 cgccgccgac cgggccggtg atcgccgcgg gttcgaccgg ttcggcgccc gccgccgccg
     6901 ccgtgctcaa ggccgtagcc aaggctccgc aaggctgcgt cgtcgtgccc ggtctcgaca
     6961 tggatctgga ccccgccgtc tgggcgacgc tgggcgatga cgaacagcat ccgcagaacg
     7021 ccctgtggcg attgctggat catggcgagg tggcgcgtga ggccgtgcgt ccatggttcc
     7081 agccccgggt tgagccggtc gttcaggccc gaggtctcgc ccgtcagcga ctgatcaacg
     7141 aagccctgcg acccgccggc gccaccagcg actggcgcaa ggagatcgcc cgcattcgcg
     7201 aggtcgccgc gccggtgcag ggcgccgatc cgattgcgct gggtctcgaa aacctcagcg
     7261 tcgtcacgtt gcggaccgag gaagacgccg ccgcgaccct cgctctgatg atgcgtgaga
     7321 cgctggagca gcgtcatcct gacggtcggc caaagacctg cgcgctggtg acgcctgatc
     7381 tcgatcttgg tcgccgcgtc gcagcccggc tggcgcgctg ggacatcatc cccgactcct
     7441 catccggcgc gccgttgtcg cggatgcctg cgggggtgct gatcgatctc gccgcccgct
     7501 ggatcgcaga cccgctgaag ccgcagacga tactggcgct gctgaagcat ccgctggtgc
     7561 gcatcgatct gggcgaggcg gatgttttcg ccgccatccg cgcgttcgag aaacatgccc
     7621 tgcgaggccc tcgcggtcgt cgctggaccg acctgcatcg ccgcctgaac gacgcggccg
     7681 agccccgcga aggccagacg
//
LOCUS       DASVTM010000307         9083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_935195,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000307 DASVTM010000000
VERSION     DASVTM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935195"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF633_08440"
     CDS             complement(<1..414)
                     /locus_tag="VF633_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8471125.1"
                     /translation="MSAAATTFKPAVEAAPETGAFQSGARLVVSTLEKLGVEVLFGYP
                     GGAIMPIYDALAGSTLRHYLCRHEQGAAFAADSYARVSGKVGVCMATSGPGATNLVTG
                     IANAMLDSVPMVCITGNVAQGVMGMDAFQEVDILGV"
     gene            complement(438..1448)
                     /gene="ilvC"
                     /locus_tag="VF633_08445"
     CDS             complement(438..1448)
                     /gene="ilvC"
                     /locus_tag="VF633_08445"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX8471126.1"
                     /translation="MKIYTNEDIRPGVIAGQKIAIIGYGSQGRAHAQNLKDSGHDVIV
                     GVRAGGKGWKNATADGLATAEPSDAVKDADIIAILTPDMAQAEVYKTQIEPFAKQGSA
                     LLFAHGFSIIYGRVTPRDDMDVILVAPKGPGDLVRREYQKGAGVPSLFAIEKDVTGKA
                     RDRAMGYAKGNGGATGGLLETTFREETETDLFGEQAVLCGGAKELVISGFNTLVEAGY
                     QPEIAYFECMHELKLIVDLFYEGGITKMNEFISETAKWGSVKSGPRVINDETRARMKQ
                     ILTEIQDGTFAREWIAENDAGKTTYDALVKEDSEQKIETVGAGLRERMAWLHAPKAAE
                     AA"
     gene            complement(1452..3209)
                     /locus_tag="VF633_08450"
     CDS             complement(1452..3209)
                     /locus_tag="VF633_08450"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX8471127.1"
                     /translation="MTTQDTPPETPVGNRRSSVVTKGPNRAAARSYLRAAGMQDADFD
                     KPMIAIVNTWSTVTPCNMHLDRLARDVRAGIIAAGGWPVDFNTIVVTDGISMGTPGMK
                     ASLISREIVADSIELAVEGHQLDGVVCIVGCDKTIPAAAMALARMDIPGLVYYGGTIM
                     PGVIGTKEVSVQEVFEAIGAHGAGTIDDEQLKIVESAVCPGAGACGGQFTANTMAMAL
                     SVMGISPMGANDVPAVDPNKGAEGHRCGQLIVDRVFAGDTARKYITRASLKNAAVAVS
                     ASGGSTNAVMHLTAIAAEAGIDFGIEDCHQACVEAPVICDLKPGGRFLASHLFAAGGT
                     RLVTQRLAEAGKIVNTPTVSGRSLFAEAADAEETPGQIVVTTVANPVMARGSYAVIYG
                     DVAPEGAIIKLTGHEVDVFEGPAAVFDSEEAAFHAVQDGSAGEGDVIIIRYEGPKGGP
                     GMREMLQVTAALKGRGVSNVALLTDGRFSGASYGFVAGHVSPEAAVGGPIALVRNGDR
                     IRIDVTNRRIDVDANLTARRKDFVLPPMAPARGVFAKYRAAVASASQGAVTIPNPPPA
                     QTPDNRVPATIKAQETEKA"
     gene            complement(3323..3478)
                     /locus_tag="VF633_08455"
     CDS             complement(3323..3478)
                     /locus_tag="VF633_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471128.1"
                     /translation="MRQSIRHDQNTRGAKAIAATRTSVGPAMLTTLILSVLLLGLLSG
                     AGWMQLT"
     gene            complement(3605..4099)
                     /locus_tag="VF633_08460"
     CDS             complement(3605..4099)
                     /locus_tag="VF633_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HEX8471129.1"
                     /translation="MFLALGAAIALALAGPAAAHKGHAGLTVVEVDPASGGVTVVHRF
                     YAHDIEPAFATIAPDAQPSLDDPAAVKALEEHLQARFRLDVDGARVALHHTDTVLAGD
                     NVRIESAGQITPASVHAVRVDLDFFPDVYEDMEQQVNVRINGITRTVVFAPGSTAQTI
                     TFGE"
     gene            complement(4108..6534)
                     /locus_tag="VF633_08465"
     CDS             complement(4108..6534)
                     /locus_tag="VF633_08465"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX8471130.1"
                     /translation="MRLGVLGVSLTAMAVAAFGSLAGAASAQVSVGPIQQTTGSFEDK
                     FRQFEGEDWPTPTDYRNASGAPGYRYWQQQVDYDIDVSLDEPTRTLTGKETITYKNNS
                     PDGLGYLWLLLDQQNYRRNSIAEMSRTYTASDTVSVNEVLRTQRMKEWEGGFNDLKIT
                     GPDGQNVPFNIVDSMLRLELPATLKTGETFTFTISFTLPMAENKVVGGRSGYECFTKP
                     NEDGNCLFEAAQWFPRLAVYSDYEGWHNAQFIGSGEFTLEFGDYDVRVTVPADHQVAA
                     TGTLQNTDILTEAQRARLEQARTADAPVYIVTPAEAAAAESRPARSGTRTWHFEADNV
                     RDFAWASSRKFVWDAMGVTQATTGRPSVMAMSFYPKEARPLWDTYSTRAIAHTIKVYD
                     QFSIPYPYPTAQSVNGPVGGMEYPMITFNGPRPIRDDSGRLTYTERAKNGLIGVVIHE
                     VGHTYFPMVINSDERQWTWMDEGLNSFVQYEAQKLWDPNFPSRGEPRDIVEYMVSQDQ
                     VPIMTNSESVLQFGNNAYAKPATALVILRETILGRDLFDRAFREYAQRWAFKRPTPYD
                     FFRTMEESSGVDLDWFWRGWFYSTDHVDISLDGVTRANLQSTDPEVRMRDARARFDEA
                     PTPTTVTNNRGIETVIERDPAVRDFYNSTDQFTVTAQARRDAASADKKADEDRRRAQG
                     FRDNIYRFNFSNVGGLVMPVIIKLTWDDGSDETVRIPAEVWRRNSKSVIWQYVSTKTL
                     KSAELDPLWETADTSRANNYFPRRIDTQSLRLSPPPPEQENRMRASDFEVSPDSTATR
                     DAPRAPAPRP"
     gene            complement(6655..7224)
                     /locus_tag="VF633_08470"
     CDS             complement(6655..7224)
                     /locus_tag="VF633_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8471131.1"
                     /translation="MSFELCSRPRNAAATREAILEAANKRFTAESYDQVGMRDIARDV
                     GVDPALISRYFGSKEDLFTSVLQGCGDGSDLTAGDRATFGERVAHDLVYGPKKEFKLR
                     WLLLMLRSITSPKAAEIIQRGSRDQFYKPFTDWVGGEDAEIRARMTAGFIMGLTVSRD
                     ITGGFEMDPGQCERFKARIATTLQGFLDS"
     gene            7391..8605
                     /locus_tag="VF633_08475"
     CDS             7391..8605
                     /locus_tag="VF633_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8471132.1"
                     /translation="MRRLKIVAASAMIAAALYGCSKTEAAGPPPPAPVTVANPLSERI
                     VDWDDFTGRFEATQTVDVRARVGGYIQSVHFRDGDYVQRGQLLFTLDPRPAQALVAAA
                     QATLAQANAQVALARTNLTRSEGLLASQAVSQAEVDTNTGSLRTAQANVAAAQATLRA
                     RQLDLEFTRVTAPVSGRASNRRSDPGNLVAGGSSAADVLTTIVSSSPIWFVFDGSEAT
                     LLKYQRQARVGAGAPVRIRLQDEGDFVHSGTLDFTDNAIDSSSGTIRLRAVVQNGDGF
                     LKPGMFGQIRVAGAGAYDALLVPDAAVSAGADQRTVSVVAADGTVTPRPVVLGPLVDG
                     LRVIRSGITAQDRVIINGGSRVQQPGQKVKANAGRITRTATTNAAPVTTAVPAATASS
                     ASALSGSVAIGD"
     gene            8613..>9083
                     /locus_tag="VF633_08480"
     CDS             8613..>9083
                     /locus_tag="VF633_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8471133.1"
                     /translation="MNISRFFIDRPIFAAVLSVFITIVGLAAYPLLPLSQYPEIAPPT
                     VTVNAVFPGASAETLAETVAAPLEQEINGVEDMLYITSSSTSDGAVAITVTFKPGTNL
                     DSAQVLVQNRVALAEPRLPDQVRQTGVVVAKASQGFLLIASVTSPDKSLGNDYLG"
BASE COUNT         1502 a         2926 c         2949 g         1706 t
ORIGIN      
        1 gacgccgaga atgtcgactt cctgaaacgc atccatgccc atgacgcctt gggcgacgtt
       61 gccggtgatg cagaccatcg gcaccgagtc caacatggcg ttggcgatgc cggtgactag
      121 attggtcgcg cccggacctg atgtcgccat acagaccccg accttgccgg agacgcgggc
      181 gtaggagtca gccgcgaagg ccgcaccctg ctcgtggcgg cacaggtagt ggcgcagggt
      241 ggaccccgcc agcgcatcgt agatcggcat gatggcgccg ccggggtatc cgaacagcac
      301 ctctacgccc agcttctcca gagtggagac gacgaggcgt gcgcccgact ggaaagctcc
      361 agtttcgggt gcagcttcga ctgcgggttt gaaggtggtg gcggcggcgc tcatcagctt
      421 gctctatcga tcctgagtca ggccgcttcc gcggccttgg gggcgtgcaa ccacgccatg
      481 cgttcgcgca ggcctgcgcc gaccgtctcg atcttctgct cgctgtcttc cttgaccagc
      541 gcgtcatagg tcgtcttgcc ggcgtcgttc tcggcgatcc attcgcgggc gaaggtgccg
      601 tcctggatct cggtcaggat ctgcttcatg cgggcgcggg tctcgtcatt gatgacgcgc
      661 gggcccgact tcaccgaacc ccatttggcg gtctcggaga tgaactcgtt catcttggtg
      721 atgccgcctt cgtagaacag gtccacgatc agcttgagct cgtgcatgca ttcgaaatag
      781 gcgatctcgg gctggtagcc ggcctcgacc agggtgttga agccgctgat gaccagttcc
      841 ttggcgccgc cgcacaggac tgcctgttcg ccgaacaggt cggtctcagt ctcttcgcgg
      901 aaggtggttt ccagcaggcc gccggtcgcg ccgccgttgc ccttggcata gcccatggcg
      961 cggtcgcgag ccttgccggt gacgtccttt tcgatggcga agagcgaagg gacgccggcg
     1021 cccttctggt attcgcgacg gaccagatcg ccggggccct tgggcgcaac cagaatgacg
     1081 tccatgtcgt cgcgcggcgt gacgcggcca tagatgatcg agaagccgtg ggcgaacagc
     1141 agggccgagc cttgcttggc gaagggttcg atctgggtct tgtagacctc ggcctgagcc
     1201 atgtcggggg tcaggatggc gatgatgtcg gcatccttga cagcgtccga aggctcggcg
     1261 gtggccagac cgtccgccgt ggcgttcttc cagcccttgc cgcccgcgcg cacgccgacg
     1321 atcacgtcgt gaccggagtc tttcaggttc tgggcgtggg cgcgaccctg agagccatag
     1381 ccgatgatgg cgatcttctg gccggcgatg acgcccggac ggatgtcttc gttggtgtag
     1441 attttcatgg ctcaggcctt ctcggtttct tgggctttga tggtcgcagg cacccggttg
     1501 tctggggttt gagccggcgg agggttggga atggtgaccg cgccttgcga ggccgaagcc
     1561 accgcagcgc ggtattttgc gaacacgccg cgggccggag ccatcggcgg caggacgaag
     1621 tccttacggc gcgcggtcag gttggcgtcg acgtcgatgc gacggttggt gacgtcgatg
     1681 cggatgcggt cgccgttgcg gaccagagcg atgggaccgc cgacagcggc ttccggcgag
     1741 acgtgtccgg cgacgaagcc gtaggaggcg cccgagaacc ggccgtcggt cagcagagca
     1801 acgttgctga cgccccggcc cttcagggcg gcggtgacct gcagcatctc gcgcatgccg
     1861 ggaccgccct tcggcccttc gtagcggatg atgatgacgt caccctcccc ggctgatccg
     1921 tcctgaacgg cgtggaaggc ggcttcctcg ctgtcgaaca cggcggcggg gccttcgaag
     1981 acatcgacct cgtggccggt cagcttgatg atcgcgcctt cgggggcgac gtcgccatag
     2041 attaccgcat aggagccgcg cgccatgacc ggattagcga ccgtcgtgac gacgatctga
     2101 ccgggcgtct cctcggcgtc tgctgcttcg gcgaacaggc tgcgccccga gacggtcggg
     2161 gtgttgacga tcttgccggc ctcggccagc cgctgggtga ccagacgggt gccgcctgct
     2221 gcgaacaggt gcgaggccag gaagcggccg ccgggcttca ggtcgcaaat gaccggagcc
     2281 tcaacgcagg cctgatggca atcctcgata ccgaaatcga tgcctgcctc agccgcgatg
     2341 gcggtcaggt gcatgacggc gttggtcgat ccgcctgagg ccgagacggc gacggcggcg
     2401 ttcttcaacg aggcgcgggt gatgtatttg cgggccgtgt cgccggcgaa aacgcggtcg
     2461 acgatcaact gaccgcagcg atggccttcg gcgcccttgt tgggatcgac ggccggaacg
     2521 tcgttggcgc ccatcggcga gatgcccatg accgacagcg ccatggccat ggtgttagcc
     2581 gtgaactgac cgccacaggc gccggcgccg ggacagacgg cgctctcgac gatcttcagc
     2641 tgctcgtcgt cgatcgtgcc cgcgccatga gcgccgatag cctcgaacac ttcctgaacc
     2701 gagacttcct tggtgccgat aacgcccggc atgatggtgc cgccgtagta gaccagaccg
     2761 gggatatcca ttcgggccag agccatggcc gccgccggga tggtcttgtc gcaaccgacg
     2821 atgcagacga cgccgtccag ctgatgccct tcgacggcca gctcgatcga gtcggcgacg
     2881 atctcacgtg agatcagcga ggccttcatg cccggcgtgc ccatcgaaat gccgtcggtg
     2941 acgacgatgg tgttgaagtc gaccggccag ccgcccgccg cgatgatgcc cgcgcggacg
     3001 tccctggcca gacgatccag atgcatgttg cagggcgtga ccgtcgacca cgtgttgacg
     3061 atggcgatca tgggcttgtc gaaatcggca tcctgcatgc ccgccgcgcg caggtacgag
     3121 cgcgccgccg cccggttcgg acccttggtc acgaccgagc tccggcggtt gccgacgggt
     3181 gtttcaggag gggtgtcttg ggtggtcact gaggtggtcg cttctggctt ggcgggggtg
     3241 atgaaggcgt cgtttcgggt atgaaaaaac ccgcttcctg tcgggagcgg gtggctgcgg
     3301 gtgatcctga ttttgcgtct gctcaggtca actgcatcca tcccgctccg gagagcaggc
     3361 cgagaaggag tacgctgaga atgagagtgg tcagcatcgc aggaccaacg ctggtgcgtg
     3421 tcgcggcgat ggccttggcc ccccgcgtgt tctggtcatg gcgaatggat tggcgcacgg
     3481 ccgctctcct gcttaccggc acataaggcc agatcaggag gctgcgcaca agctgttgtt
     3541 ggaataaact tccaatattc cgtcgaaaaa gcgccgctgg ggggaaacga accccttcag
     3601 cgcattattc gccgaaagtg atcgtctgcg ccgtgcttcc cggggcgaaa accaccgtgc
     3661 gggttatgcc gttgatacgg acgttcacct gctgctccat gtcctcatag acgtccggga
     3721 agaaatccag atcgactcgc acggcatgga ctgaagctgg cgtgatttgc ccggcggact
     3781 cgatccgcac gttgtcgccg gctagcaccg tgtcggtgtg atgcagggcc acacgagcgc
     3841 cgtcgacgtc gagacggaag cgcgcctgaa gatgctcctc cagcgccttc accgctgcag
     3901 ggtcatcgag agatggttgg gcgtcaggcg cgatcgtggc gaaggcgggt tcgatatcgt
     3961 gcgcataaaa ccggtggacg acggtgacgc ctccgctggc gggatcgacc tcgaccaccg
     4021 tcagcccggc atggcccttg tgagccgccg ccggtccggc gagcgccaag gcgatggcgg
     4081 ctcccagagc caggaacact ctccgcttca cggccggggc gccggtgcac ggggagcgtc
     4141 acgcgtggcg gtcgaatccg ggctgacctc gaaatcggag gcgcgcatgc ggttttcctg
     4201 ctcgggcggc ggcggcgaca accggaggga ctgggtgtcg atgcggcgcg ggaaatagtt
     4261 gttggcgcgg ctggtgtcag ccgtttccca aagcggatcg agttcggcgg acttcagcgt
     4321 cttggtcgag acgtactgcc agatcaccga cttcgagttc cggcgccaga cctcggctgg
     4381 aatgcgcacg gtctcatcag acccgtcgtc ccacgtcagc ttgatgatga ccggcatcac
     4441 cagaccgccg acgttggaga agttgaagcg gtagatgttg tccctgaagc cctgcgcacg
     4501 ccggcggtct tcgtccgcct tcttgtcggc cgaggcggcg tcgcgacggg cctgcgccgt
     4561 gaccgtgaac tggtctgtcg agttgtagaa gtcgcggacc gccggatcgc gctcgatcac
     4621 ggtttcgatt ccgcgattgt tggtcaccgt cgtcggcgtg ggggcttcat cgaaacgggc
     4681 gcgggcgtcg cgcatccgca cctcgggatc ggtcgattgc aggttggcgc gggttacccc
     4741 gtccagcgag atgtcgacgt ggtcggtcga atagaaccaa ccgcgccaga accagtcgag
     4801 atcgacgccc gagctctcct ccatcgtgcg gaagaagtca tatggcgtcg gacgcttgaa
     4861 cgcccagcgc tgagcgtatt cgcggaaggc gcggtcgaac aagtcccggc ccaggattgt
     4921 ctcacgcagg atcaccagcg ccgtcgccgg cttggcgtag gcgttgttgc cgaactggag
     4981 caccgactcc gagttggtca tgatcggcac ttggtcctgc gagaccatgt attcgacgat
     5041 gtcgcggggc tcgccacgcg aggggaagtt cgggtcccaa agcttctggg cctcgtactg
     5101 gacgaagctg ttcagcccct cgtccatcca cgtccactgg cgctcgtcgg agttgatgac
     5161 catcgggaaa taggtgtggc ccacctcgtg gatcaccacc ccgatcaagc cgttcttggc
     5221 gcgctcggta taggtcagac ggccgctatc gtcgcggatc ggacgcgggc cgttgaaggt
     5281 gatcatcggg tattccatcc cgccgaccgg accgttcacc gactgggcgg tcggataggg
     5341 atacgggatc gagaactggt catagacctt gatcgtgtgg gcgatggcgc gcgtcgaata
     5401 ggtgtcccag agcgggcgcg cttccttcgg atagaaggac atggccatga cgctggggcg
     5461 gccggtcgtt gcctgcgtca cgcccatggc gtcccagacg aatttgcgcg agctggccca
     5521 ggcgaagtcg cggacgttgt cggcctcgaa atgccaagtc cgcgtacccg accgcgcggg
     5581 acggctttca gcggcggcgg cctcggcggg ggtcacgatg taaaccggcg catcggcggt
     5641 gcgcgcctgt tccagacggg cgcgctgcgc ctcggtcagg atgtcggtgt tctggagcgt
     5701 gcctgtcgcc gcgacctgat ggtccgcagg cactgtgacg cgaacatcat agtcaccgaa
     5761 ctcgagcgtg aactcgcccg agccgatgaa ctgggcgtta tgccagcctt cgtaatccga
     5821 atagacggcc agacgcggga accactgcgc cgcttcaaac aggcagttgc catcttcgtt
     5881 cggcttggtg aaacactcgt agccactgcg acccccgacg accttgttct cggccatcgg
     5941 cagggtgaag ctgatggtga aggtgaaggt ctcgccggtt ttcagcgtcg ccggcagctc
     6001 tagccgcagc atggagtcga cgatgttgaa cgggacgttc tggccgtccg gaccggttat
     6061 cttcaggtcg ttgaagccgc cttcccattc cttcatgcgc tgggtgcgca ggacctcgtt
     6121 gacgctcacc gtgtccgagg ccgtgtaggt ccgcgacatt tcggcgatgg agttccggcg
     6181 atagttctgc tggtcgagca gcagccacag atagccgagt ccatcgggcg agttgttctt
     6241 gtaggtgatg gtttccttgc cggtcagcgt ccgggtcggc tcgtcgagcg agacgtcgat
     6301 gtcgtaatcg acctgctgct gccaatatcg atagccggga gcgccggacg cgttgcgata
     6361 gtcggtcggg gtcggccagt cctcgccttc gaactggcgg aacttgtcct cgaaggatcc
     6421 cgtcgtctgc tgaatgggac cgacgctgac ctgagccgaa gccgctcccg ccaacgatcc
     6481 gaacgccgcg acggccatcg ccgtcagact tacacccaac acacccaaac gcatccgcaa
     6541 ccccgtcggt attcaactac agcctgccgc gaccttacgg ctgaaacttc ggtgcgcaac
     6601 ccacggctcg tgcgctgtga cggaatgccc ggcccgatta ccgggcgggc tgagttagct
     6661 gtcgaggaag ccctgcaggg tcgtcgcaat gcgcgccttg aaccgctcgc actggcccgg
     6721 gtccatctcg aacccgccgg tgatgtcgcg gctgacggtc agacccatga tgaagcctgc
     6781 ggtcatccgt gccctgatct ctgcgtcctc accaccgacc cagtcggtaa acggcttgta
     6841 gaactggtca cgcgatccgc gttggatgat ctccgctgcc ttgggcgagg ttatcgagcg
     6901 cagcatcagc agcagccagc gcagcttgaa ctccttcttc ggaccataga ccagatcgtg
     6961 cgccacgcgc tcaccgaacg tcgcccggtc accggccgtc aggtctgatc cgtcgccgca
     7021 gccttgcagc accgaggtga acaggtcctc cttggagcca aaataccggg agatgagcgc
     7081 gggatcgact ccgacgtcgc gcgcgatgtc gcgcatgccg acctgatcat agctttcggc
     7141 tgtgaagcgc ttgttggcgg cttctagaat ggcctcacgg gtggccgccg cattccgcgg
     7201 tctgctgcaa agctcgaaac tcaagttgat gtcctcaaaa gccccaagag gagtatggct
     7261 atgtcatcgc ctgttgacaa ccatctcgca tttgcacaaa aacaagtcat caggtgttga
     7321 cctagggcgg cgacgcccca tttgcgcctt tgtctcgcct accacacaat ttccactcag
     7381 gagtccgcgc atgcgcagac tgaagatcgt cgccgcatcc gcaatgattg cggccgcact
     7441 atatggttgt tccaaaaccg aggctgcggg tccgccccct cctgcacccg tcaccgtcgc
     7501 aaaccccctc agcgaacgca tcgttgactg ggacgacttt accgggcgct tcgaagccac
     7561 ccagacggtc gatgttcgcg cccgcgtcgg cggctatatc cagtccgtcc acttcaggga
     7621 tggcgactat gtccagcgcg ggcagttgct ttttaccctt gatccccgcc cggctcaggc
     7681 gcttgtcgcg gccgctcagg cgacgctggc tcaggccaac gcccaggtcg ctctcgcccg
     7741 caccaacctg acccgttcgg aaggcctgct ggcctctcag gcggtgtccc aggctgaagt
     7801 cgacaccaac accggctcgc tgcgcaccgc gcaagccaat gtcgctgccg cccaggctac
     7861 cctgcgcgcc cgtcaactgg atctcgagtt cacccgcgtc acggcgccgg tctcaggtcg
     7921 cgcatcgaac cgccgttccg acccgggcaa cctggtcgcc gggggctcgt cggctgcaga
     7981 cgtcctgacc accatcgttt cgtcctcgcc gatctggttc gtcttcgacg gctccgaagc
     8041 gaccctgctg aagtatcagc gtcaggcccg cgtcggcgcc ggcgctcccg tccgcattcg
     8101 tctgcaggac gagggcgact tcgtgcactc aggtactctc gacttcaccg acaacgccat
     8161 cgacagctct tccggcacca tccgccttcg cgccgtggtc cagaacggcg acggcttcct
     8221 gaagcccggc atgttcggtc aaatccgcgt cgccggcgcc ggcgcctatg acgccctgct
     8281 ggttcccgat gccgccgtca gcgcgggcgc cgatcagcgc accgtctcgg tcgtcgctgc
     8341 cgacggcacc gtcaccccgc gtcctgtggt tctcggcccg ctggtcgacg gcctgcgcgt
     8401 gatccgctcc ggcatcaccg ctcaggaccg cgtcatcatc aacggcggtt cgcgggtcca
     8461 gcagcccggt cagaaggtga aggccaacgc tggccgcatc acccgcacgg cgacgacgaa
     8521 cgccgccccc gtaaccactg ccgtccccgc cgccacggcg agctcagcca gcgccctgtc
     8581 gggttccgtg gcgatcggcg actgaaacag acatgaacat ctcacgcttc tttattgatc
     8641 ggccgatctt cgcggccgtt ctctcggtgt tcatcaccat cgtcgggctc gcagcctatc
     8701 cgctgctgcc cttgtcccaa tatccagaga ttgccccgcc gacggtcacc gtgaatgcgg
     8761 tgttccccgg cgcctcggcc gaaacgctcg ctgaaacggt cgccgcgccg cttgagcagg
     8821 aaatcaacgg cgtcgaggac atgctctaca tcacctcttc gtcgacctcg gacggcgcgg
     8881 tggcgatcac cgtcaccttc aagcccggca cgaacctcga ttcggctcag gtgctggtcc
     8941 agaaccgggt cgcgctggct gaaccccgct tgcccgatca ggtccgccag accggcgtcg
     9001 tggtggccaa ggcttcacag ggcttcctgc tgatcgcctc ggtcacgtcc ccggacaaga
     9061 gcctgggcaa cgattatctg ggc
//
LOCUS       DASVTM010000308         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_615065,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000308 DASVTM010000000
VERSION     DASVTM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615065"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF633_08485"
     CDS             <1..214
                     /locus_tag="VF633_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX8471134.1"
                     /translation="HLAEAFPDAEIAIDDLAGDGDHYRARIVSTAFTGLPRVRQHQLV
                     YAALKGKVGGELHALALETATPSTGG"
     gene            218..1144
                     /locus_tag="VF633_08490"
     CDS             218..1144
                     /locus_tag="VF633_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8471135.1"
                     /translation="MRYRPFGATGTSISNVTLSLGMDAIARGPEAASELIYAALEAGI
                     NSYRLENSDPVLAEVVGQALASVDRKLLVVSLTLGRRDGRRSDASRDFSAESMTHAID
                     RALHVSGLGWIDLALLERPGELELPLSSLTALKALRATGRVRYLGVFGDDEVMDAYVS
                     TGAFDVLATPSNVNSPWRIRSRIRAAQEQDMAILTYDYYPEALSSLRKVEAATRPKKG
                     FFGIGGGGRPKNDPLAGAGTFAFLHQTPHWDAESICLAHVMNDPAVASVLIQARDVPR
                     LEALAAIPDRDMPPGLSAQIEMARVGTATQAA"
     gene            1260..1619
                     /gene="grxD"
                     /locus_tag="VF633_08495"
     CDS             1260..1619
                     /gene="grxD"
                     /locus_tag="VF633_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX8471136.1"
                     /translation="MTDQAVTQTVDPVHAFIASTIADNAVVLFMKGTPDAPRCGFSAL
                     SVQILDQIESPFVGVDVLQDDALRDGIKAFTDWPTIPQLYVKGEFVGGSDIIREMFQS
                     GELQALLVEKGVALGQD"
     gene            1626..>1998
                     /locus_tag="VF633_08500"
     CDS             1626..>1998
                     /locus_tag="VF633_08500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX8471137.1"
                     /translation="MARSPLQPREDREVFTLPLTILPEHIDGNGHVNNVVYVGWLQEA
                     GTAHWNARFDEQTRAKWSWVALRHEIDYLRGLMPDSTAVARTWVGDPQGPRFARFVRI
                     EDEQGRVCAQGHSEWVLVDAAT"
BASE COUNT          325 a          705 c          616 g          352 t
ORIGIN      
        1 ccatctggcg gaagcctttc ccgacgccga gatcgccatc gatgatctgg ccggggatgg
       61 tgaccattac cgtgcgcgca tcgtttcgac cgccttcacc ggcctgcccc gcgtccgcca
      121 gcaccagctc gtctatgcgg ctctcaaggg taaggtcggc ggcgaacttc acgctcttgc
      181 tctggaaacc gcaacccctt ctaccggagg ctgacaaatg cgttaccgtc ccttcggcgc
      241 caccggaaca tcgatctcca acgtcaccct cagccttggc atggacgcca tcgcgcgcgg
      301 ccccgaggca gcgtcagagc tgatttatgc ggctctggaa gccgggatca acagttacag
      361 gctcgagaac tcggatcctg tgctggctga ggtcgtcggt caggctctgg cttccgtcga
      421 tcgcaaactg ctggtcgtca gtttgacgct agggcgccgt gacggacgtc gcagcgacgc
      481 cagccgtgac ttctccgccg agagtatgac ccacgccatc gacagggcgc tgcacgtctc
      541 aggtcttggc tggatcgacc tggctctgct ggaacggccc ggcgaactcg agttgcccct
      601 gtcctcgttg accgcgctca aggctctgcg cgccaccggc cgggttcgtt atcttggcgt
      661 tttcggcgac gatgaggtca tggacgccta tgtgtcgacg ggcgccttcg acgtgctggc
      721 cacgccctcg aacgtcaact cgccctggcg catccgctcg cgcattcgcg cggcccagga
      781 acaggacatg gcgatcctga cgtacgacta ctatccggaa gctctgagct cgctcaggaa
      841 ggtcgaggct gcgacccggc cgaagaaggg cttcttcggc atcggtggcg gtggacgacc
      901 gaaaaacgat ccccttgcgg gcgcggggac cttcgcgttc cttcaccaga cgccgcactg
      961 ggacgccgag tccatttgtc tggcgcacgt catgaacgat cccgccgtcg ccagcgtcct
     1021 gatccaggcg cgcgacgtcc cgcgtctcga ggctctggca gcgatccccg atcgggatat
     1081 gccgcccggc ctgtccgccc agattgaaat ggcccgcgtc ggcacggcga cccaggccgc
     1141 ctgaaccagg cgatttggcg ccatcaagcc cgcgtgacag cgggtcgcgc gagggcgcgc
     1201 ggccttgacc ggcccggtca tcgtccctag ctgacgggct tgaacgagag gcctctccca
     1261 tgactgatca agccgtcacc cagaccgtcg accccgtcca cgccttcatt gcctcgacga
     1321 tcgctgacaa cgccgtcgtc ctgttcatga aaggcacgcc ggatgcgcca cgctgcggat
     1381 tctcggccct gtcggtgcag atcctcgacc agatcgagtc tcctttcgtc ggcgtcgacg
     1441 tgttgcaaga cgacgccctg cgcgacggca tcaaggcctt caccgactgg ccgaccatcc
     1501 cacagctcta tgtgaagggc gagttcgtcg gcggctccga catcatccgc gagatgttcc
     1561 agtccggcga gcttcaggcc ctgctggtcg aaaagggcgt ggccctcggt caggactgat
     1621 cccgcttggc gcgctcgccg ctccagccgc gtgaggaccg cgaagtcttc accctgcccc
     1681 tgaccatcct gcccgagcac atcgacggca acggccacgt caacaacgtg gtctatgtcg
     1741 gctggcttca ggaggccggc acggcgcact ggaacgcgcg cttcgacgag caaacccgcg
     1801 cgaaatggtc gtgggtcgcc ctgcggcacg agatcgacta cctgcgcggc ctgatgccgg
     1861 actcaaccgc cgtcgcccgc acctgggtcg gcgatccgca gggcccgcgc ttcgcccgct
     1921 tcgtccgcat cgaggacgag cagggccgcg tctgcgccca gggccacagc gaatgggtgc
     1981 tggtcgacgc cgccaccc
//
LOCUS       DASVTM010000309         8674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_511025,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000309 DASVTM010000000
VERSION     DASVTM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511025"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VF633_08505"
     CDS             <1..960
                     /locus_tag="VF633_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8471138.1"
                     /translation="IGGVEHAVLHLLYARFITRALTDAGMMSIKEPFAGLFTQGMVVH
                     ETYRRADGAWVEPTDVDLANDAGVRSARQLSTGETLTIGDIEKMSKSKKNVVAPQEIL
                     NSHGVDAGRLFVLSDSPPERDVQWTPGGVEGASRFVQRVWTEFDSYDASIAGEDAANA
                     SLVRETHKAIKAVSEGVEGFRFNSAIAKLYAFLATVRDAKGTGGDARRQALSALARLI
                     APFTPHLAEEGWSRLSEEGMVLDAPWPIYDPALAADDEVVLPIQISGKRRSEITAARG
                     AASVDVEAAALANPAIQAYLSANGVAVKKVIVVQDRIVNLVVG"
     gene            960..1439
                     /gene="lptE"
                     /locus_tag="VF633_08510"
     CDS             960..1439
                     /gene="lptE"
                     /locus_tag="VF633_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX8471139.1"
                     /translation="MRALVACVVLLASAVLAGCGFTPMYVTGGSPSITRIAVAAEDNR
                     LGYRLREQLEDALASDRSAPPLYRLTTVTEQSRRPLGRRIDDTATRYELTVRVAWTLT
                     PASGGTPLVGTETSTSTYAAADQPYAAIAAQQDGEDRAAAELARLIRVDILRALSGL"
     gene            1446..2498
                     /gene="holA"
                     /locus_tag="VF633_08515"
     CDS             1446..2498
                     /gene="holA"
                     /locus_tag="VF633_08515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX8471140.1"
                     /translation="MILAKRPDIDRFLARPDPGIRAVVIHGKDRSGVSERAQVLCKAI
                     TPDLNDPFNVTVLTDSDIDGDEARLEEALTALSMIGGRRLVRVRLGGEKVSIDKMLAA
                     ALKTHSDGGYNPDAMLVIEAGALGRDSALRKAAETAKAAAGITCYEDETGDVARMTRE
                     ALAADKVGLTSDALDRFVSRLPRERGLMRQELERLALYIGPGSGRTIDVEELDAHLGV
                     EPDASLQDAALQAFGGRPAPSQAGLRRALAEGESAVMAVRQASIHLGKLRRINVLQGA
                     GAGAKEAAKAAGVFWKQEAEMLRQARSWRLEELDMVLDSINVADVATKTTGMPEGLIA
                     ERLLLEISARARRLGL"
     gene            complement(2495..2689)
                     /locus_tag="VF633_08520"
     CDS             complement(2495..2689)
                     /locus_tag="VF633_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX8471141.1"
                     /translation="MSDAFNTPTSVDPRLLEVLVCPVTRGVLTYDREANELVSKGAKL
                     AFPIRDGVPIMLAEEARQLD"
     gene            complement(2808..3464)
                     /locus_tag="VF633_08525"
     CDS             complement(2808..3464)
                     /locus_tag="VF633_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX8471142.1"
                     /translation="MAQGYVKAADLPQVIPVFPLPGAILLPRGQLPLNIFEPRYLNMI
                     DDAMAGDRLIGLIQPAGGPPSLPSLTAVGCAGRITSFAETSDGRYLVTLTGVCRFRVT
                     TELPIQAPYRQIRATYAAFESDLTVPSGEPFDRDTFLGALKAYLERRQLEIDWETADA
                     APQEALINSLSMALPFEAAEKQALLESPTLDDRCLTLTALMQIDAAEPGDGDSPTSMQ
                     "
     gene            complement(3479..4402)
                     /locus_tag="VF633_08530"
     CDS             complement(3479..4402)
                     /locus_tag="VF633_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HEX8471143.1"
                     /translation="MTFADPKTDADKSDQGDMIVEGSDAAFMTDVIEASRSRPVIVDF
                     WATWCGPCRQLTPALEKAVRASGGAVKLVKIDIDKNPAYAGQLRVQSIPTVYAFVDGQ
                     PVDGFQGAVPESQIKAFIEKLTGGEGGAESVDNDVEQLLSLGEESLHLSDFGGAAQAF
                     AHVLTLDPENQKAIAGMARVYLAGDDTEQAAQTIAMAPADSTEPSVVGVRAQLALAAN
                     APSGETAALEAAVSANPDDHQARYDLALAQAATGDFKASVDSLLTIVQADREWNEQAA
                     RKQLLTVFEAAGAMSEVARDGRRRLSSILFA"
     gene            4605..5237
                     /locus_tag="VF633_08535"
     CDS             4605..5237
                     /locus_tag="VF633_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471144.1"
                     /translation="MKSVLIMALGATTLAAAGSAFAGDRETAGYATASLNTSTIIEGE
                     RWGGGATLLAIDRNCECSVPTVAVPFYTVRPPAPPIRVRAPGLRISSAPVFAPSPAIY
                     IQGPPIYVDAPPIQVAPAQVYLEAPDVHVRPSEVSVAPPEIHFTPAGVDHPDEHCCDV
                     ASPPSASNNLAPPPMGGPVGGAATYPAPASYPAPAIGTSSGYSQEPGERG"
     gene            complement(5351..6094)
                     /gene="pyrF"
                     /locus_tag="VF633_08540"
     CDS             complement(5351..6094)
                     /gene="pyrF"
                     /locus_tag="VF633_08540"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX8471145.1"
                     /translation="MTLLDPNVSHTAPHRTADPRLICALDTATVAEARALVDQLGDAV
                     NFYKIGLQLFASDGMTLARQLKGEGRRIFLDWKLHDIGATVEKAAAALAGAGCDFLTV
                     HARPQVMTAAARGVAGSDLKVLGVTVLTSLDIADLIADDHSLSPALLVEHRVRQALDS
                     GIDGVVASPHEAARVREIAMAGGRPDFLIVTPGVRPVGASMDDQARAATPERALRDGA
                     THLVVGRPITAMTDPRAAAFAIAGSIATL"
     gene            6178..6867
                     /locus_tag="VF633_08545"
     CDS             6178..6867
                     /locus_tag="VF633_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX8471146.1"
                     /translation="MNALDLGVNLFVALFALVDPIGNIPIFAAATAGASVRQRLSVSG
                     LICLFIAAFLALFFFTGIGLLQFFGISLAAFRIAGGILLLLLGLDMTRGDFLSMFADA
                     DAVADAKDVRGYARRRFQRLIVPFAMPLLIGPGAISTIIIQAGEAQKFGPAGTVAGLA
                     AIGAAALATFVIFAFTGPLSRILGEVGMVIVVRVLGLILCALAIQFILAGLGEAIPGM
                     FAGSVTAPYPH"
     gene            complement(7009..7827)
                     /locus_tag="VF633_08550"
     CDS             complement(7009..7827)
                     /locus_tag="VF633_08550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8471147.1"
                     /translation="MAQPALSTRKTALITGASAGIGAALARVYAEKGWNLILVARRAA
                     PLQALARELTIDHGATATCLTADLAIPDEPHRLVAALAARGLTVDALINNAGFSRTTG
                     FLATSPDDHAAMIDVMLTAPIALTRLLLPGMVERGFGRVLNVASLAGLMPATGGDTLY
                     GPIKSFLIKASAGLHLELAGTGVHVTALCPGYTLTEFHDVNGSREAVSSAYPAWMWQT
                     AEHVARVGYAAVEANRARVIPGWRNTLLAAIPKLLPDAVALTIIGGHAKRLKRI"
     gene            complement(7830..8300)
                     /locus_tag="VF633_08555"
     CDS             complement(7830..8300)
                     /locus_tag="VF633_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8471148.1"
                     /translation="MSDSQRFPVAAHTLAYLAHRDAFDAARAMPSAILAASIPTNPVV
                     IRRVTALLAKAGLIATRPGASGGSWLLRQPEEIRLDQVLRAVNGCAHLGSPPRGAEGC
                     PISEHIPRQVAKALTAADSAAHEALSRITIADLLAESPASLAAFAPHKSCPVAA"
     gene            8480..>8674
                     /locus_tag="VF633_08560"
     CDS             8480..>8674
                     /locus_tag="VF633_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471149.1"
                     /translation="MAGDTPRGDGAPPAPPTSITDTTGAAAGAHAAAHGGHGGHAAAK
                     VGFWGLTIGAIGVVFGDIGTS"
BASE COUNT         1425 a         2838 c         2874 g         1537 t
ORIGIN      
        1 atcggcgggg tcgagcacgc ggtgctgcac ctgttgtacg cccgcttcat cacgcgagcg
       61 ctgaccgacg ccggcatgat gtcgatcaag gagccgttcg cgggcctgtt cacccaaggg
      121 atggtggtcc acgagaccta ccgccgggct gacggcgcct gggtcgaacc cacggacgtc
      181 gatctggcca acgacgccgg tgtgcgttcg gcgcgccagc tctcgactgg cgaaaccctg
      241 accatcggcg atatcgagaa gatgtcgaag tccaagaaga acgtcgtcgc gcctcaggaa
      301 atcctgaact cgcacggcgt tgacgccggt cgcctgttcg tcctgtccga cagtccgccc
      361 gagcgggacg tgcaatggac cccgggcggc gtcgagggcg ccagccggtt cgtccaacgc
      421 gtctggaccg agttcgacag ctatgatgct tcgatcgcgg gcgaggacgc cgccaacgcg
      481 tcgcttgttc gcgagaccca caaggccatc aaggccgtgt ccgagggagt cgaaggcttc
      541 cgcttcaact ccgcgatcgc caagctctat gccttccttg cgaccgtacg cgacgctaag
      601 ggcacgggcg gcgatgcgcg ccgtcaggcc ttgtcggcgt tggcccgcct gatcgctccg
      661 ttcacgcctc acctggccga agaagggtgg tctcgattga gcgaagaggg aatggtgctg
      721 gacgcgccat ggcccattta cgatcccgca cttgccgccg acgacgaagt ggtgctgccg
      781 atccagatct ccggcaagcg ccgcagcgag atcacggctg cacgcggcgc cgccagcgtc
      841 gatgtggagg cggccgccct cgccaacccc gctattcagg cctatctctc ggccaatggc
      901 gtcgctgtaa agaaggtgat cgtggttcag gatcgcatcg taaatctggt ggtcggctga
      961 tgcgcgcgct tgtcgcctgc gtcgtcctgt tggccagcgc cgttttggcc ggctgcggtt
     1021 tcacgccgat gtacgtcacc gggggctcgc cctcgatcac gcgcatcgcc gtcgcggccg
     1081 aggataaccg gctgggctat cggctgcgtg agcaactgga agacgccctg gcctcggatc
     1141 gcagcgcgcc accgctttat cggctgacca cggtgaccga gcagagccgt cgtccgctcg
     1201 ggcgtcggat cgacgacacg gccacgcgtt acgaactgac agtccgagtc gcctggaccc
     1261 tgacgcctgc gagcggcggc acgcctctgg tcggcacgga gacctcgacg tcgacctatg
     1321 ccgccgccga ccagccctat gccgccatcg ccgcccagca ggacggtgag gaccgcgccg
     1381 ccgccgaact ggcgcggctg atccgggtgg atatcctgcg agccctgtcg ggactgtaaa
     1441 cctccatgat cctcgccaag cgcccggaca tcgaccgttt tctggctcga cccgatccgg
     1501 gcatccgggc cgtggtcatc catggcaagg accgttcggg cgtttccgaa cgggcgcagg
     1561 tcctgtgcaa ggcgatcacg ccggacctga atgacccctt caatgtcacg gtcctgaccg
     1621 acagcgacat cgacggcgac gaggcacgcc ttgaggaagc cctgacggcg ctgagcatga
     1681 tcggcggacg gcgtctggtg cgcgtgcggc tgggcggcga gaaggtctcg atcgacaaga
     1741 tgctggccgc agcgctcaag acccactccg acggcggcta caatcccgac gccatgctgg
     1801 tgatcgaggc aggcgcgctg gggcgcgact ccgccctgcg caaggccgcc gaaaccgcca
     1861 aggccgccgc cggcatcacc tgctacgagg acgaaaccgg cgacgtcgcc cggatgacgc
     1921 gcgaagctct cgccgccgac aaggtcgggc tgacctctga cgcgctggat cgtttcgtca
     1981 gccgcctgcc gcgtgagcgg ggcctgatgc ggcaggagct cgagcggctc gctctctaca
     2041 tcggccccgg ctcgggccgg acgatcgacg tcgaggaatt ggacgcccat ctcggcgtcg
     2101 agccggatgc ctcacttcag gacgcggcct tgcaggcgtt cggaggccgt ccggcaccgt
     2161 cgcaggcggg tctcaggcgc gctctggccg agggcgagag cgcggttatg gccgtacggc
     2221 aggcctccat ccatctgggc aagctgcggc ggatcaatgt gcttcagggc gcgggagccg
     2281 gcgccaagga ggccgccaag gccgctgggg tgttctggaa acaggaagcc gagatgctgc
     2341 gtcaggcccg ctcgtggcgg ctcgaagagc tggatatggt tctggacagc atcaacgtcg
     2401 ccgatgtcgc caccaagacg accggcatgc ctgagggttt gatcgccgaa cggctgctgc
     2461 tcgaaatatc cgcccgggcc cggcggctcg gtctttagtc gagctgacgc gcttcctccg
     2521 ccagcatgat cggcacgccg tcgcggatag ggaaggccag cttcgccccc ttcgatacca
     2581 gctcgttggc ctcgcggtca taggtcagca cgccacgcgt cacggggcac accagcacct
     2641 caaggaggcg gggatcaacc gaggttgggg tgttgaaggc gtctgacatt gggggcttcg
     2701 ccgggggtta gggagtagag agtagggagt agcaggcggg agaggtgcag gctaacgcgt
     2761 tcgcggccac cctactgcct aacctctact ccctactgcc tccttcttca ttgcatcgat
     2821 gtcggtgaat cgccgtcacc cggttccgcc gcgtcgatct gcatcagggc ggtcagcgtc
     2881 aggcagcggt cgtccagtgt cggcgactcc agcaaggcct gcttttcggc ggcttcgaag
     2941 ggcagggcca tcgaaagact gttgatcaag gcttcctgtg gcgcggcgtc ggccgtctcc
     3001 cagtcgatct ccagctgacg acgctccaga taggccttga gcgcgccgag gaatgtatcg
     3061 cggtcgaacg gctctccgga tgggaccgta aggtccgact cgaacgccgc ataggtcgcc
     3121 cggatctggc gataaggcgc ctggatcggc agttcagtcg ttacgcggaa gcggcataca
     3181 ccggtcagtg tgacgaggta gcgtccgtca gaggtctcgg cgaagctggt gatccgtccg
     3241 gcgcatccaa cggccgtcag gctgggtagg gaagggggcc cccccgctgg ctggataaga
     3301 ccgatgagcc ggtctcccgc catggcgtcg tcgatcatgt tcagatagcg gggctcgaag
     3361 atattgagcg gcagttgccc gcgcggcagc aggatagccc cgggcagggg aaacaccggg
     3421 atgacctgcg gcagatcggc ggctttcaca taaccctgtg ccatccggtt tctctcactc
     3481 acgcgaacag gatggaggaa aggcggcggc gaccgtcgcg ggcgacctcg ctcatcgcgc
     3541 ccgccgcctc gaagaccgtc agcaactgct tgcgggcggc ctgctcgttc cattcgcggt
     3601 cggcttgaac gatggtcagc aggctatcca cggaagcctt gaagtcgccg gtcgcagcct
     3661 gcgccagcgc cagatcatag cgtgcctgat gatcatccgg attcgccgac actgcagcct
     3721 ctagcgcagc ggtctcgccg gacggcgcgt tggccgccaa ggccagctgg gcacgcaccc
     3781 cgacgaccga gggctcggtg gaatccgcgg gcgccatcgc gatggtctgg gcggcctgtt
     3841 cggtgtcgtc tcccgccaga tagacgcgcg ccattccggc gatcgccttc tggttctcgg
     3901 ggtccaaagt cagcacgtgg gcgaaggctt gcgccgcacc gccgaagtcg gaaaggtgga
     3961 gggattcctc acccaacgac agaagttgtt cgacatcgtt gtcgacgctt tcggccccac
     4021 cctcgccacc ggtcagcttt tcgatgaagg ccttgatctg gctttcaggc accgcgccct
     4081 ggaaaccgtc gaccggttgg ccatctacga aggcgtagac ggtggggatc gattggaccc
     4141 gcagctgccc tgcataggcc gggtttttgt cgatatcgat cttgaccagt ttcacggcgc
     4201 cgccggatgc ccgcacggcc ttttcaagcg caggggtcaa ctggcggcaa gggccgcacc
     4261 atgtcgccca gaaatcgacg atcacaggcc gcgagcgcga cgcctcgatc acatcggtca
     4321 taaacgcggc gtcggatccc tcgacgatca tgtcgccttg atcagacttg tcggcgtcgg
     4381 ttttggggtc ggcgaaggtc atcaaagcct gctgtgattg tctgggctta acgcgagagg
     4441 cgtcagatgg tctcgcgggg cgacaaactc aaggatttct cccgtgtgag ggtgtcactc
     4501 tcctgcaacg cgaaaaatat caccgatatt cctcttaatg ggtgcaagcc aaaccgttgc
     4561 tgttcatgct tcattaaggt ctggatgact ggggagacat catcgtgaaa tcagttctga
     4621 tcatggcgct gggcgccacc actctggcgg cggccggctc ggcctttgca ggagaccgcg
     4681 agactgcggg ttatgccaca gcgtcgctca acacgtccac gatcatcgag ggcgaacgtt
     4741 ggggcggggg ggcgaccctt cttgcaatcg accgcaactg cgagtgctcg gtaccgaccg
     4801 tagccgtgcc cttctacacc gtgcgtccgc cggcgccgcc catccgggtc cgggctcctg
     4861 gcctgcgcat ttcgtcggct ccggtgttcg ctccgtcgcc tgcgatctac attcagggcc
     4921 cgccgatcta tgtcgacgcc ccgccgatcc aggtcgctcc ggcccaggtt tacctggaag
     4981 cgccagatgt ccacgtccgc ccgtcggaag tgtccgtcgc tccgccagaa atccacttca
     5041 cgcccgccgg tgtcgaccat ccggacgagc attgctgcga cgtcgcctca ccgccgtcag
     5101 cttcgaacaa ccttgcgccg cccccgatgg gcggccctgt cggcggtgct gcgacctacc
     5161 cggcgcccgc cagctatccc gctcctgcca tcggcacgag ctcgggctac tcgcaagaac
     5221 ccggcgaacg cggctaaacc ttcgacctca cggttcaggg accccgtggg cggcccaccg
     5281 acaggtgggc cgcccttttc gtgcatccag ggcagagccg cagttgttcg tccgccagtc
     5341 cgcttgaccg ctaaagagtg gctatggacc cggcgatggc gaaagcggcg gcccgagggt
     5401 cagtcatggc tgtgatcggg cggccgacca ccagatgggt ggctccgtcc ctaagggcgc
     5461 gttcgggtgt tgcggcacgg gcctgatcgt ccatcgacgc accgacgggc cgcactcctg
     5521 gcgtgacgat gagaaagtcc ggtcgccccc cggccatggc gatctctcgc acccgggcgg
     5581 cctcgtgggg gcttgcgacc acgccgtcta tcccgctgtc caaagcctga cgcaccctgt
     5641 gctcgaccag cagggccggc gacaggctgt ggtcgtcggc gatcaggtcg gcgatgtcga
     5701 ggctggtgag gacggtgacg cccagcacct tgaggtcaga tcctgccaca ccgcgggcgg
     5761 cggcggtcat cacctgcggc cgcgcgtgga cggtcaggaa gtcgcatccg gcgccggcaa
     5821 gcgcggcggc ggccttttct acggtcgcgc cgatgtcgtg cagcttccag tcgaggaaga
     5881 tccggcgacc ttcgcccttg agttggcggg ccagcgtcat gccgtctgaa gcgaacagtt
     5941 gcagcccgat cttgtagaaa ttgactgcgt ccccgagttg atcgaccaga gcccgcgcct
     6001 ctgcgaccgt ggccgtatcc agcgcgcaga tcagccgcgg atctgcggtt cggtgtggtg
     6061 cggtgtggga gacgttcggg tcaagcaggg tcatcgacgg actcgcattg agcggctaga
     6121 ccggacgcag aggtgcgtcg ggcagggccg gcggcggatt tgggctgaga aacaggcatg
     6181 aacgcgctcg atctgggggt caacctgttc gtggccttgt tcgcccttgt cgaccctatc
     6241 ggcaatatcc cgatcttcgc ggccgcaacc gctggggcca gcgtgcgtca gaggctgtcg
     6301 gtctcgggcc tgatctgcct cttcatcgcc gcctttctgg ctctgttctt tttcaccggc
     6361 atcggactgc tgcagttctt cggcatttcg ctcgcggctt tccggatagc gggcggcatc
     6421 ctgttgttgc ttctgggtct cgacatgacc cgcggggact tcctcagcat gttcgcggat
     6481 gcggatgcgg tggccgacgc caaggacgtg cgcggttatg cacgccggcg attccagcgt
     6541 ttgatcgtcc ctttcgccat gccgctgctg atcgggcccg gcgccatctc gaccatcatc
     6601 attcaggcgg gggaggcgca gaaatttggt ccggcaggga ctgtcgcggg actggccgcc
     6661 atcggggctg cggccctcgc caccttcgtg atcttcgcct tcacaggacc gctgagccga
     6721 atcctcggcg aagtcgggat ggtcatcgtc gtgcgggtac tggggttgat cctgtgcgct
     6781 ctggcgattc agttcatcct cgcaggcctc ggcgaggcga tccccggcat gttcgccggc
     6841 tcggtcactg cgccgtatcc acattgaggg agggagaagg cagtaggcag ataggcaata
     6901 ggcagtagga gatcccttgc agacccgctg ctggccgtcc cttgccactg cctactgcct
     6961 actgcctact gcctactgcc tactgcctac tgcctactgc cttcttctct aaatccgctt
     7021 cagccgtttt gcgtgtccgc cgatgatcgt cagggcgaca gcgtccggca gcagcttcgg
     7081 aatagcggcc agcagagtgt tgcgccagcc gggaatgacg cgggcccgat tggcttccac
     7141 cgccgcatat cctacccggg ccacatgctc cgcggtttgc cacatccagg ccggataggc
     7201 cgaagacacc gcctcacggc tgccgttgac gtcgtggaat tcagtcaggg tatagccggg
     7261 gcacaatgcc gtgacgtgga cgccggtccc ggcgagctcc agatgcagac ccgctgaagc
     7321 cttgatcagg aagctcttga tcggcccgta caacgtatcg ccccccgtgg ccggcatcag
     7381 tcccgccagc gacgcaacat tgagcacccg accgaagccg cgctcgacca tacccggcaa
     7441 caacaggcgc gtcagagcga tgggcgcggt cagcatgacg tcgatcatag ccgcatggtc
     7501 gtcggggctc gtcgccagaa agcctgtcgt gcggctgaag cccgcgttgt tgatcagggc
     7561 atccacagtc agaccgcgcg ctgcgagcgc cgccaccagc cgatgtggct cgtcggggat
     7621 tgcgaggtct gcagtcagac aggtggcggt ggcgccatgg tcgatggtca gctcgcgagc
     7681 cagcgcctga agcggcgctg cacgacgggc caccaggatc aggttccagc ccttttctgc
     7741 atagacccgg gccagggccg cgccgatgcc cgcggacgcg ccggtgatca gcgctgtctt
     7801 tctcgtggac agagcaggct gcgccaagct tacgccgcta cggggcagga cttatgaggc
     7861 gcaaatgccg cgagcgaggc cgggctttcg gccagaagat cggcgatcgt gatcctgcta
     7921 agcgcttcgt gagccgcgct gtcggcggcg gtcagggcct ttgcgacctg acgggggatg
     7981 tgttcactga tcgggcagcc ctcggcgccg cgcggggggg agccgagatg cgcgcagccg
     8041 ttgacggccc ggagcacctg atccagccga atctcctcag gctgacgtag cagccaggat
     8101 cccccggatg cacccggacg ggttgcaatc aggccggcct tggccagcag agcggtcact
     8161 cgccggataa cgaccgggtt ggtcgggatc gatgccgcca ggatggcgct cggcatcgcc
     8221 cgggcggcgt cgaacgcgtc cctgtgagcc agataggcca gggtgtgggc ggcgacggga
     8281 aagcgttggc tgtctgacat ttgtgctcgg tttcagccca caaggtaagc gttcccggac
     8341 ggattcacaa atccacgtgc tcgtttcgaa gaacgcggtg caactgaaac cgctgacgca
     8401 cgattgaacc ggtcgcggaa tgagcctaca ggcgctccgc tttgatgggc gcgcggaccg
     8461 cgcggcccgg aggattttca tggctgggga tactccccga ggcgacggcg ctccccccgc
     8521 tccgcccacc tcgatcaccg acacgactgg cgcagccgcc ggcgcccatg cggctgccca
     8581 tggcggtcat ggcggccacg ccgcagccaa ggtcggcttc tggggcctga ccatcggcgc
     8641 catcggcgtc gtgttcggcg acatcggcac cagc
//
LOCUS       DASVTM010000310        11374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_454485,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000310 DASVTM010000000
VERSION     DASVTM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454485"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF633_08565"
     CDS             complement(<1..414)
                     /locus_tag="VF633_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8471150.1"
                     /translation="MAAKLGGSGGGRYHVDQNAEINVTPFVDIMLVLLIIFMVASSVA
                     TVSVEVKLPIAVAKAQENPPKPVFISVTTDGGVFIGDGQSSFATLGSDLTSQIGRRDP
                     SKERIFIRADQQTRYGDFMQAMNALQDAGFYSVALV"
     gene            complement(420..1313)
                     /locus_tag="VF633_08570"
     CDS             complement(420..1313)
                     /locus_tag="VF633_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8471151.1"
                     /translation="MLDSKKTNILLAMAGAAVLMASSPVLAQDTAAAPAATAPAAAAP
                     AAAPTPAAAPADDAETGAEAAGGHGGEITPVSMFMEATPVVKVVMVGLLLASIFSWTL
                     LIIKLIEFGSLNRKTDNFLESYRSARTIADMRKVATSDEFDGNPLADMAAAATEEIEL
                     SRQAGLAVTGDHRDSVLYRAGSAVSAVQSGLASRLSGGQQFLASTGSIGPFVGLFGTV
                     YGIMNSFIGIVQSNTTNLAAVAPGIAEALLATGIGLFAAIPAVVFYNYFNTRIAAYGT
                     RADGFAAELLNALSRQLDKGA"
     gene            complement(1822..1897)
                     /locus_tag="VF633_08575"
     tRNA            complement(1822..1897)
                     /locus_tag="VF633_08575"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1862..1864),aa:Phe,seq:gaa)
     gene            complement(1984..2166)
                     /gene="yacG"
                     /locus_tag="VF633_08580"
     CDS             complement(1984..2166)
                     /gene="yacG"
                     /locus_tag="VF633_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX8471152.1"
                     /translation="MATCPICKRHPSEPKYTPFCSSRCADVDLGRWFTGGYAIPVRDD
                     EPTDDGDGPDRSRRED"
     gene            complement(2171..3196)
                     /locus_tag="VF633_08585"
     CDS             complement(2171..3196)
                     /locus_tag="VF633_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX8471153.1"
                     /translation="MSGEIEVFLDETPGETRGLISRDGRYTHLFIERDGDVAALKLGA
                     RSIGRVAEIDSGLKGAFVELVGGAAGFLPLGKSHDLRQGQKIEVEVSAEPREMKVAAL
                     RRIGAGEGDPRLVKAGPKVVETLTKLAPGVEIQTGVFAIEAAWEAEEEALSGSVVLAG
                     AGLDLAIQRTRAMVTVDIDHAGKKAAGGAKQANLLGLHHAARLIRLNRWGGLVAFDLV
                     GTGQDGDALLKAARDAFGGDPEVVFGPLNRFGVLMLSLPWRRRPLDEILNGSKTSPTL
                     ESRAIAIVRRLRHAMLSNTSAPRLFAQCTPQEAEIAAPLVAKLGPRAHLRSDPAAVPG
                     RCIIEES"
     gene            complement(3201..3779)
                     /locus_tag="VF633_08590"
     CDS             complement(3201..3779)
                     /locus_tag="VF633_08590"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX8471154.1"
                     /translation="MNPKPELVLASASPRRIELLALIGITPDRIDPADIDETPQRDET
                     PSRLAVRLATSKAAVVAERSPDSVVLGADTVVAVGRRLLEKPADEAEAAKFLKLLSGR
                     NHRVFTGVAVAFGGKVISRVVDTRVSFKVLSKAEIADYVAGGDWRGKAGGYGIQGPAG
                     AFVVKIAGSYPAVMGLPLFETVNLLSGAGWRK"
     gene            complement(3787..4008)
                     /gene="infA"
                     /locus_tag="VF633_08595"
     CDS             complement(3787..4008)
                     /gene="infA"
                     /locus_tag="VF633_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX8471155.1"
                     /translation="MAKEELLEFPGVVSELLPNATFRVLLEDNNHEIIAHTAGKMRKN
                     RIRVLAGDKVLVEMTPYDLTKGRITYRFK"
     gene            complement(4186..4662)
                     /locus_tag="VF633_08600"
     CDS             complement(4186..4662)
                     /locus_tag="VF633_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEX8471156.1"
                     /translation="MSEHRLSAVDLDMATLPPATAEIEHERRVAIFDLIEKNSFEPEG
                     APGGPYKLKLSLQDNRLVFDIAGEGAHEDFTRTYALSLTPLKSVLKDYLMICDSYYEA
                     LRGSSPSQIEAVDMGRRGLHNEGAELLKTRLDGKVAIDHETARRLFTLVCALYRRG"
     gene            complement(4659..5171)
                     /locus_tag="VF633_08605"
     CDS             complement(4659..5171)
                     /locus_tag="VF633_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEX8471157.1"
                     /translation="MSTDAELKAQLEEAFGQAPPPTGGANAPLRLLAEDAEDLAIISA
                     ALQDSILRPADIVWERGARRLTIELSRFCWECGGTRVRAGMQFGDVHAVKSRGLPRLP
                     DAPLELLAIDFEEVEAPGGRITLMFAGGGDLRLEVECLDAVVVDLSDRWEARIAPTHL
                     EDAVPSENAT"
     gene            complement(5161..6465)
                     /gene="murA"
                     /locus_tag="VF633_08610"
     CDS             complement(5161..6465)
                     /gene="murA"
                     /locus_tag="VF633_08610"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8471158.1"
                     /translation="MDSIAVQGGAQLYGEIPISGAKNSAIKLMAATILTDQPVRLTNM
                     PRLADTKFLGQLLRQFGVVVTESDGPDGQQTLFDAGDITSTFAPYDLVRQMRASFNVL
                     GPLLARTGHAKVSLPGGCTIGARPVDLHIDALSKLGAVIELEEGYVSATAPKGLRGAE
                     IEFPFVSVGATEHTLMAAVLARGTTVLRRAAREPEIGDLARCLIAMGAKIKGVDTDTL
                     VITGVTSLTGTDWSVIPDRIEMGSYACAVAMAGGEVRLTKARPELIDSLTEKMIAAGV
                     EVTPTADGVLIKRDRALRLRAVDVATEVYPGFATDLQAQFMALMTTADGVSTIHENIF
                     ENRFMHAPELARLGADISVHAGEARVTGVEWLRGAPVMATDLRASVSLVIAGLAAEGE
                     TTIGRVYHLDRGFERLEEKLGACGAEIRRIHGPPVEGPGDEH"
     gene            complement(6598..6774)
                     /locus_tag="VF633_08615"
     CDS             complement(6598..6774)
                     /locus_tag="VF633_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471159.1"
                     /translation="MTETPKTPGDTPSPKALPQQRTPEQTAKSARLAKALRDNLRRRK
                     IAHPAASKPSSQNH"
     gene            6872..6946
                     /locus_tag="VF633_08620"
     tRNA            6872..6946
                     /locus_tag="VF633_08620"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6904..6906,aa:Thr,seq:ggt)
     gene            complement(7035..7607)
                     /locus_tag="VF633_08625"
     CDS             complement(7035..7607)
                     /locus_tag="VF633_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471160.1"
                     /translation="MLIALLAMTALTVADDPQGVVTTAPAGHQSVATDAVLPAPTEAP
                     SASSQTITPHGLTTAEQIDRWVGQRDPSAKPFSQASAGGTGRWAGDSWGRVDDRKMHG
                     EVSAAIGTGDFSEYSAAVSIPVGESARVDLEYRQTKNGYGQYGYPGAYPGYGRYGAYG
                     YGRPFSAAGQSQSFSIGYSSDSSARDPYYR"
     gene            complement(7657..8151)
                     /gene="msrB"
                     /locus_tag="VF633_08630"
     CDS             complement(7657..8151)
                     /gene="msrB"
                     /locus_tag="VF633_08630"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8471161.1"
                     /translation="MTDTSTVPQPVRSSSGYDLTPPSEGQRVALEAGLNAEEKRVLLS
                     HGTEAPFCGVLLGEKRPGVFCCRECGLPLFKATTKFESGTGWPSFTQPVDEDHVTAIV
                     DRSYGMSRTETTCARCGSHQGHVFPDGPPPTGLRYCINSVALEFVPEGSPLPDPLMRG
                     DGIA"
     gene            complement(8432..9913)
                     /locus_tag="VF633_08635"
     CDS             complement(8432..9913)
                     /locus_tag="VF633_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471162.1"
                     /translation="MIHRLIAASAATLALIAASGSAQAQDFRELARQDLQAAHDQLRA
                     NHPAAVVGGAASQTFNGWIDAGLATALGKAGQVNSGDSHAYLMRYYANGFRDSNISMT
                     PSFEGAGPFFGISWPGFTTGWRNGKYVVTYIKPGVRGTPPLGATLVDCVGKTAEDMAK
                     DKLEGWEGDISSEAGRVTTAPYLLWNRNNPFASGVPSVCNFQVGRGRPREFRLTPQPA
                     NVADLEAAYRATVYVPGATPLSIEQVNGRPWINVHSFADTAGWDAFNAQVLAQQASFA
                     NGFVLDLRGARGQSYTSTARGYGLANRIWTPEFTVSRQPAAGEITYRATAGNRQWYAD
                     TLGRMQADPRFVQESAPVIEETQAIVAAFDAAIASGQQSFTLAGRAATPDTGAANPIK
                     GPVLVLVDAGCAGGCLDTLDLLSRLPNVRIAGSTTATDSIFIEPTMTRLPSNYSDLSY
                     GHKAWTSRQRANNTAFVPAAGLVYAGNPTDEAAVRTWVGTLMQ"
     gene            complement(10103..10834)
                     /gene="lipB"
                     /locus_tag="VF633_08640"
     CDS             complement(10103..10834)
                     /gene="lipB"
                     /locus_tag="VF633_08640"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX8471163.1"
                     /translation="MLADPLSPAQSSTESVVFRSDGPPVEWAVSSGYVDYEPAVAAME
                     ARVAAIVAGEAPEIVWLLEHPPLYTAGVSSKDADLLDAGRFPVHRTGRGGQFTYHGPG
                     QRVAYVMLDLNRRGRDVRALVRGLEQWVIGGLAEFGVEADVREGRVGVWVERKGAGWS
                     REDKIAAIGVKVRRWVTFHGISLNVEPDLDHFGGIVPCGISDHGVTSLVDLGVPATMD
                     EADAALKTSFQRVFGPVTTASPPEV"
     gene            10884..11171
                     /locus_tag="VF633_08645"
     CDS             10884..11171
                     /locus_tag="VF633_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX8471164.1"
                     /translation="MCQGVPGFEEYPLSQIDLVDVEISVVLGRSVLPMSQLLKMGRGA
                     VIPLDASEHDEVWILANNHPVARGEIEIREDRIAITVTRPADVYDFMAGAA"
     gene            11254..>11374
                     /locus_tag="VF633_08650"
     CDS             11254..>11374
                     /locus_tag="VF633_08650"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8471165.1"
                     /translation="MTSDVRSYRDLLVWQRAMDVAVAVYDLSRTWPRDELFGMT"
BASE COUNT         2012 a         3683 c         3655 g         2024 t
ORIGIN      
        1 gacgagcgcg acgctgtaga agcccgcatc ctgaagcgca ttcatggcct gcatgaagtc
       61 accatagcga gtctgctggt cagcccggat gaagatgcgt tccttgctag gatcgcgcct
      121 gccgatttgg ctggtcagat cggaccccag ggtggcgaag ctcgattgcc cgtcaccgat
      181 aaaaacgccg ccgtccgtgg tcaccgagat gaagaccggc ttgggtgggt tctcttgagc
      241 cttcgcgaca gcgatgggaa gtttcacctc gaccgacacg gtagcgaccg aagacgccac
      301 catgaagatg atgagaagga ccaacatgat atcaacgaac ggagttacgt tgatttccgc
      361 gttctggtcg acgtgatacc taccgccgcc cgagcctccg agtttggcgg ccatgcggct
      421 tacgctccct tgtcgagctg acgcgagagg gcgttcagca gttcagccgc gaagccatcg
      481 gcgcgggtgc cgtaagcggc gatgcgggtg ttgaagtagt tatagaacac gaccgccggg
      541 atagcggcga acaggccgat accggtggcg agcagggcct cggcgatacc gggagcaacg
      601 gcggccaggt tggtcgtgtt ggactgaacg ataccgatga acgagttcat gatgccgtac
      661 acggtgccga acaggccgac gaacggaccg atggagccgg ttgatgcgag gaactgttga
      721 ccacccgaca gacgcgaggc gaggccagat tgaaccgccg agacggctga cccggcgcgg
      781 taaagaaccg agtcgcgatg atcaccggtg actgccaggc cagcctgacg ggacagttcg
      841 atttcttccg tagctgcggc ggccatgtcg gccagcgggt tgccgtcaaa ctcgtccgag
      901 gttgcgacct tgcgcatgtc cgcaatagtg cgggcgctgc ggtaggactc gaggaagtta
      961 tcggtcttgc ggttcaggct gccgaactcg atcagcttga tgatcagcag ggtccaggag
     1021 aagatcgaag cgagcagcag gcccaccatg acgaccttaa ccaccggggt ggcctccatg
     1081 aacatgctga cgggcgtgat ctcgccgccg tgaccgccgg ctgcttcggc gccggtctca
     1141 gcatcgtcgg caggcgcggc tgccggggtc ggcgctgcgg ccggagccgc cgctgccgga
     1201 gcggtcgcgg caggagccgc tgcggtatcc tgggccagga cagggctgct cgccataagg
     1261 acagcggcgc cggccatggc gaggaggatg ttcgtcttct tgctatcgag catgtgtcgc
     1321 cagttcctgc tttttcggtc tgagctcgtg gaccctagag acaggccgca tcatgcgacc
     1381 gcccctccat gaatttgttt tgttccgaca tctgaccgca atggccgaac ggcggaacgc
     1441 ctcctatcgc atccggttgc ccctggggga accttcgcgt tggaatgccc ccgggacgat
     1501 gtccctcagg ccttcgttcg caaacgctta agaaaggcgt ctagcgtctt tggcgaaccg
     1561 ttatcagagc gtcaagagcg cattggcgct tttcgcccgt tgagcgaaag caacgggcga
     1621 aacccataag cgacgggcga aagcgacatt ttgatgatac cccgacggca taatctgatg
     1681 ctcgaatgac atgatagccg ccgccactcg acaggggcgg taaccggtgg acatcgcgcc
     1741 acagccggga ggcgaaccag gcaaatatat tcgcgctatg atgaagtaga acagccgccg
     1801 ctcggccaaa cggacggagg atggtgcctg ggggcggatt cgaaccaccg acacgcggat
     1861 tttcaatccg ctgctctacc aactgagcta cccaggcgaa cgcttccgat cgcgagagcg
     1921 gggtctataa gcgagccgat ccggggtgtc cagcactccg tcggctgaag tttcacagta
     1981 gcgtcagtcc tcccgccggc tacggtcggg accatccccg tcgtccgtcg gttcgtcgtc
     2041 gcgcacggga atcgcatagc cgcccgtgaa ccaccgcccc agatcgacgt cggcgcaacg
     2101 gctcgagcag aacggcgtgt atttcggctc gctcggatgg cgtttgcaga tcgggcaggt
     2161 cgccatgagt tcagctctcc tcgataatgc agcggccggg cacggcagcc gggtcagatc
     2221 tcaagtgggc gcgcggaccc agtttcgcca ccagaggcgc agcgatttca gcctcttgcg
     2281 gagtacactg cgcgaacaga cgcggagcgg aggtattcga cagcatcgcg tgccgaagcc
     2341 gtcgcacgat cgctatggcc cggctctcca aggtcggact tgtcttggac ccgttcagta
     2401 tctcgtcgag aggcctccgc ctccagggca aggacagcat cagcacgccg aatcgattca
     2461 gcgggccaaa caccacttcg ggatcgccgc cgaatgcgtc gcgtgcggcc ttcaacaagg
     2521 cgtcgccatc ctgtcccgtc ccgaccagat cgaacgccac cagtccgccc cagcggttga
     2581 gccggatcag ccgcgcggcg tggtgcagtc cgagcagatt cgcctgcttt gccccgcccg
     2641 ccgccttctt gcccgcgtga tcgatatcga ccgtcaccat cgcccgggtg cgctggatcg
     2701 ccagatcgag acccgcgccc gccagcacga cactgccgga cagcgcctcc tcttcggctt
     2761 cccaagcggc ttcgatggcg aagacccccg tctggatttc cacaccgggc gccagcttgg
     2821 tcagcgtctc cactaccttg ggaccggcct tgaccaggcg aggatcaccc tcccccgcgc
     2881 cgatccgtcg cagggcggcg accttcattt cccggggttc tgcggagact tcgacctcga
     2941 tcttctggcc ctgtctcaga tcatgagatt tgccgagcgg aaggaagccg gccgcccctc
     3001 cgacgagttc gacgaaggct cctttgaggc ctgagtcgat ttccgccact cgcccgatcg
     3061 agcgcgcgcc gagcttcagt gcggcaacgt cgccgtctcg ttctataaac agatgagtgt
     3121 agcggccgtc gcgggagatc agaccgcgcg tctcgccggg cgtctcgtcg aggaagacct
     3181 cgatctcccc gctcatcgca ttatttccgc cagccggcgc cggacagcag gttgaccgtc
     3241 tcgaacaggg gcaggcccat gaccgccgga taggatcccg cgatcttgac cacgaaggcc
     3301 ccggccggtc cctgaatacc gtatccgccc gccttgccgc gccagtcgcc accggcgacg
     3361 taatcggcga tctcggcttt ggacagaacc ttgaagctca cgcgggtgtc gaccacccgg
     3421 ctgatgacct tgccgccaaa cgccacagcg accccggtga agacccggtg attgcgaccc
     3481 gacaacagct tcaggaattt cgcggcctcc gcttcatcag cgggcttttc cagcaaccgc
     3541 cgcccgaccg cgaccacggt atccgcgccc agaaccacgg agtccggaga ccgttcggcg
     3601 acgaccgcag ccttggacgt ggccagccgg acggccagcc gcgacggtgt ctcgtcgcgc
     3661 tggggcgttt cgtcgatgtc ggcaggatcg atgcgatcgg gcgtgatccc gatcaacgcc
     3721 agcagttcga tgcggcgcgg gctggccgaa gccagcacaa gttcaggctt ggggttcacc
     3781 cgagccttac ttgaagcggt aggtgatgcg gcccttggtc aggtcatagg gcgtcatttc
     3841 gaccaggacc ttgtcgccgg ccagcacgcg gatgcggttc ttgcgcatct tgccggcggt
     3901 atgggcgatg atctcgtggt tattgtcctc gagcaggaca cggaacgtcg cgttcggcag
     3961 caattcgctg acgacaccgg gaaactcgag cagttcttcc ttagccatgc ggcctctcac
     4021 gccccgtgaa tacgaatcga ggctcgcgcc gggcaaagcg cgagccgtaa gaaggggcgg
     4081 ctaatgcacc aaaaaggctg tctagtcgag ttatcctcac catcgctgtc acgatcggga
     4141 gccgcaccgt cctccgcgtg cggatggtga cgggttcttg cccgcctatc cccgccggta
     4201 cagcgcgcag accagagtga acaaccgccg cgcggtttcg tggtcgatgg cgaccttgcc
     4261 gtccaaccgc gtcttgagca gttcggcgcc ttcgttgtgc aaaccgcgac ggcccatatc
     4321 gacagcctcg atctggctgg gcgaggagcc ccgcaaagcc tcgtaatagc tgtcgcagat
     4381 catcagatag tccttcagca cacttttcag cggcgtcagc gacagggcgt aggtgcgcgt
     4441 gaagtcctcg tgagccccct ccccggcgat gtcgaacacc agccggttgt cctgcaagga
     4501 gagcttcagt ttgtagggac cgccgggcgc gccctcgggc tcgaagctgt tcttctcgat
     4561 caggtcgaag atggcgacgc ggcgctcatg ctcgatctcg gcggtcgccg gcggcagggt
     4621 ggccatgtcg agatcgacgg cggaaagcct gtgctcgctc atgttgcatt ttccgaagga
     4681 accgcatctt caagatgggt cggcgcgata cgcgcctccc agcgatccga cagatcgacc
     4741 acgacggcgt caaggcattc gacttcgagc ctcaaatcac cccctcccgc gaacatcagg
     4801 gtgatcctgc cgcctggagc ctcgacctcc tcgaaatcga tcgccaacaa ttccagcgga
     4861 gcatccggca ggcggggaag gccccggctc ttgaccgcgt gaacgtcgcc gaactgcatt
     4921 ccggcccgga cacgcgtccc gccgcattcc cagcagaacc gactcaactc gatggtcagg
     4981 cgacgcgcgc cgcgttccca tacgatgtcc gctggacgca ggatcgagtc ctgcaacgcc
     5041 gccgagataa tcgcgagatc ctcggcgtcc tcggccaaca accgcaaggg cgcattcgcc
     5101 cctcccgtcg ggggcggcgc ctgcccgaag gcttcttcga gttgggcctt gagttcggcg
     5161 tcagtgctca tcgcccggtc cttctacggg tgggccgtgg atccggcgaa tctcggcccc
     5221 acaggctcca agtttctcct cgagacgttc gaacccgcga tccagatgat agacgcgtcc
     5281 gatcgtggtt tcaccctctg cggccagacc ggcgatcacg agactgaccg acgcccgcag
     5341 atcggtcgcc attacaggtg cgccacgcag ccattcgacc ccggtcaccc tggcttcacc
     5401 cgcatgaacc gagatatcgg cgcccagtcg cgccagctcg ggcgcgtgca tgaaccggtt
     5461 ctcgaagatg ttctcatgga tggtggagac gccgtctgcc gttgtcatca gggccatgaa
     5521 ctgggcttgc agatcagtgg cgaagcccgg atagacctcc gtcgccacgt ccacggcccg
     5581 gagccgcagc gccctgtcgc gcttgatcag cacgccgtcc gccgtcggcg tcacctcgac
     5641 gcctgcagcg atcatctttt ctgtcaacga gtcgatcagc tcgggtcgcg ccttcgtcag
     5701 ccgaacctcg ccaccggcca tggcgacggc gcaggcgtag gaccccattt cgatcctgtc
     5761 cggaatcacg gaccagtctg tgccggtcag cgaggttaca ccggtgatga ccagggtgtc
     5821 ggtgtccacg cccttgatct tcgcccccat cgcgatcagg cagcgggcca gatcgccgat
     5881 ctcaggctcc cgggcagcgc gcctcaacac cgtggtgcca cgggccagca cggcggccat
     5941 caatgtgtgt tcggtcgccc cgaccgagac gaacggaaac tcgatctccg cgccgcgcag
     6001 acccttgggc gcagtcgcgg agacatagcc ctcctccagc tcgatgaccg cgcccagctt
     6061 ggacagggcg tcgatatgca ggtcgaccgg ccgcgcaccg atggtgcaac cgcccggcag
     6121 cgagaccttg gcgtggcccg tgcgcgccaa cagcggcccc agcacgttga acgacgctcg
     6181 catttgccgg accagatcat agggcgcgaa ggtcgaggtg atatcaccag cgtcgaacag
     6241 tgtctgttgc ccgtcaggac catcgctctc ggtcacgacg acgccgaact ggcgcagcaa
     6301 ctgaccgagg aatttcgtgt ccgcgaggcg cggcatgttg gtcagccgca cgggctggtc
     6361 cgtgaggatg gtggccgcca tcagcttgat cgccgaattc ttggcgccgc tgatcgggat
     6421 ttccccatat agctgggcgc cgccctgtac cgcgatactg tccatgtcga tcctggcgcc
     6481 agtccgcgac gtgcggttcg gcgagccggc tatctagcgc gcgagccggg tcttgcgaaa
     6541 ggcccgctca agcgccgggc gatgactttg cgttgccata acgcatggcc tgcggcttca
     6601 gtggttctgg ctgctcggct tcgatgcggc gggatgcgcg atctttcggc ggcgcagatt
     6661 gtcgcggagg gccttggcca gccgggccga cttggcggtc tgctcaggcg tacgttgctg
     6721 aggcagtgct tttggagagg gcgtatcgcc cggcgtcttc ggcgtttcag tcattcgtca
     6781 tcaagtcccg aaaatctttc gtcgcacaag cgcgcttttc aggcttcccc cccgccggtc
     6841 gtttggctat acggccgctt cctcggaaaa ggccgccgta gctcagtggt agagcgcatc
     6901 cttggtaagg ctgaggtcgt gggttcgatt ccccccggcg gcaccattcg agaacagaaa
     6961 ggcccgatcc tcacggatcg ggcctttttt attccccgga catcgggaaa gcgttgcagt
     7021 cgtggcggcg ctcgtcagcg gtagtaagga tcgcgcgcgc tggagtcgct gctgtagccg
     7081 atgctgaaac tctggctctg gcccgcagca gagaatggcc gcccgtaacc ataggcgccg
     7141 tatcggccgt atcccggata agcgccggga tagccgtact ggccataacc gttcttggtc
     7201 tggcgatact ccagatcaac gcgcgcggat tcgcccacag gaatcgatac cgccgccgaa
     7261 tattcggaga agtcgccggt gcctatcgcg gcgctgacct cgccatgcat ctttcgatcg
     7321 tcgacccgtc cccacgaatc accggcccaa cgcccggtcc cgccggcgct tgcctgcgag
     7381 aacggtttgg cagacgggtc ccgttgtccg acccaccggt cgatctgctc ggctgtcgta
     7441 aggccgtgag gcgtaatggt ctggctggac gcgctaggcg cctcggttgg ggccggcagg
     7501 acggcgtcgg tcgctacgga ttgatgtcct gccggagcgg tcgtgacaac gccctggggg
     7561 tcgtcggcaa ccgtcagggc ggtcatggca agaagagcga tcagcatgcg gtcaactccg
     7621 ggtacagggg cagtctagcc gacattgcgg ccaaggtcag gcgataccgt ctccgcgcat
     7681 caacggatcg ggcagcgggc tgccttcagg cacgaattcc agcgccactg agttgatgca
     7741 gtagcgcaga ccggtcggag gggggccatc agggaagacg tggccctgat ggctgccgca
     7801 gcgcgcgcag gtcgtctctg tccggctcat gccgtatgag cgatcgacga tggccgtgac
     7861 gtggtcctcg tctacgggct gggtgaagct gggccagccc gtgccgctct cgaacttggt
     7921 ggtggccttg aacagcggca ggccgcattc gcggcagcag aacacgcctg gacgtttctc
     7981 gcccaacagc actccgcaga acggggcctc ggtgccgtga gacagaagca cgcgcttctc
     8041 ctcggcgttc agacccgctt ccagagcgac ccgctggcct tcgcttggcg gtgtcaggtc
     8101 gtagccggat gaagaacgga cgggctgcgg aacggtcgat gtatcggtca tgaatttcaa
     8161 atggggatcg aaaggcctcg gttccagtct tcacctctcc atttgcgaag gcgaatggag
     8221 agaacagatc gaggcgcttg cggcgatcag gtgagggcga caccgcaaga aaagggggcg
     8281 accggccagc accgtgtcgc cccctactga tcgctccgcg atctgtcctc cccgtcgccc
     8341 ttcggcgaca gggaggtgaa cgcgcactct tactggaact tctgcgctca agcagcgagc
     8401 gaccgcgtcg cgaccgttag cgcccttccg cttactgcat caacgtcccg acccacgtcc
     8461 gcacggcggc ttcgtcggtg gggttgcccg catagaccag acccgcagcc ggaacgaagg
     8521 cggtgttgtt ggcgcgctgg cgtgaggtcc aggccttgtg accgtagctg aggtccgaat
     8581 agttggacgg caggcgagtc atggtcggct cgatgaagat cgagtcggtg gccgtggtcg
     8641 aaccggcgat ccggacgttc ggcaggcggc tcagcaggtc gagcgtatcg aggcaaccgc
     8701 cggcgcaacc ggcgtcgacc agcacgagga ccgggccctt gatcgggttg gcggcgcccg
     8761 tatcaggcgt agcggcgcga ccggcgaggg tgaaactctg ctggcccgaa gcgatggctg
     8821 cgtcgaaggc tgcgacgatc gcctgcgttt cctcgatcac gggggcgctt tcctgcacga
     8881 agcgtggatc ggcctgcatg cggcccagcg tgtcggcgta ccactgccgg tttccggcgg
     8941 tggcgcggta ggtgatctca ccggcggccg gttggcggct gacagtgaac tcgggcgtcc
     9001 agatccggtt ggcaaggcca tagccacgcg ccgtcgaggt gtaggactgg ccacgggcgc
     9061 cgcgcaggtc gagcacgaag ccgttggcga agctggcctg ttgcgccagc acctgggcgt
     9121 tgaaggcgtc ccagccggcg gtgtcggcga acgagtggac gttgatccac gggcggccgt
     9181 tgacctgttc gatcgagagt ggcgtcgcgc ccggtacata cactgtcgcg cggtaagcgg
     9241 cttcgagatc ggcgacgttg gcgggctgcg gggtcaggcg gaactcacgc gggcggccac
     9301 gtccgacctg aaagttgcag accgacggca cgccgctggc gaaggggtta ttgcggttcc
     9361 acagcagata aggcgcggtg gtgacgcggc cggcttccga agagatgtcg ccttcccagc
     9421 cttcgagctt gtccttggcc atgtcctcgg ctgtcttgcc cacgcagtcg accagcgtcg
     9481 cgcccaacgg cggcgtgccg cgcacgccgg gcttgatgta tgtcaccaca tatttgccgt
     9541 tgcgccagcc ggtcgtgaat ccgggccagc tgatgccgaa gaacggtccc gcgccctcga
     9601 agctgggcgt catcgagatg ttcgagtccc ggaaaccgtt ggcgtagtag cgcatcagat
     9661 aggcgtggct gtcaccgctg ttgacctgtc cggccttgcc cagagcggtg gcgagacccg
     9721 cgtcgatcca gccgttgaag gtctggctgg ccgccccgcc gacgacagcg gcgggatggt
     9781 tggcgcgcaa ctggtcgtgc gccgcttgca gatcctgacg ggccagttcg cggaaatcct
     9841 gggcctgggc cgatccggat gcagcgatga gggcgagggt ggcagccgag gctgcgatca
     9901 gacgatggat caaggcagat ctccgggaag ttaacgacgc gttgccggtg ttaaagccga
     9961 tccacgcccg ccttgccaag cccgaacacc cctgccgcga cctcatgcgc cacgataggc
    10021 aggaatgccc ggtttccgcg gttcaggccg caatttcttc gcgcgattga ggccgtcgga
    10081 cggagtgctc aaccccttcg ggtcagacct ctggcggtga agccgtcgtc accggtccga
    10141 acacgcgctg aaaactggtt ttcaacgcgg cgtcggcctc gtccatcgtt gcaggaacgc
    10201 caaggtcgac cagactagtg acgccgtggt ccgatatacc gcacgggacg atgccgccga
    10261 aatggtcgag gtcgggttcg acgttcaggc tgatgccgtg gaaggtcacc cagcggcgca
    10321 ccttgacccc gatggcggcg atcttgtcct cgcggctcca gcctgcgcct tttcgttcga
    10381 cccagacgcc gacgcggccc tcgcgcacat ccgcctcgac gccgaactcg gccaggccgc
    10441 cgatgaccca ctgctccagt ccccgcacca gcgcccgcac gtcgcgcccg cgccggttca
    10501 gatccagcat cacataggcg accctctggc cgggcccatg ataggtgaac tgcccgcccc
    10561 gccccgtccg gtgaaccggg aaccgcccgg catccagcag gtccgcgtcc ttcgacgaca
    10621 ccccggccgt atagagtggc ggatgttcaa gcagccacac gatctccggc gcctcgcccg
    10681 ccacaatcgc ggcgacacgc gcctccatcg ccgctaccgc cggctcgtag tcgacatagc
    10741 cggaggacac cgcccattcc acgggcggcc cgtcggatcg gaaaaccacc gattccgtgg
    10801 acgattgagc gggacttaac gggtcggcaa gcatgatgga ctcaatgtgt ccgccggtgc
    10861 gtccgcgcaa gacccaggca gacatctgtc agggcgtccc gggatttgaa gagtacccct
    10921 tgagccagat cgatctagtc gatgtcgaga tttccgtagt gcttggccgc tcggtcctgc
    10981 cgatgtcgca acttctcaag atgggtcgtg gcgcggtgat cccgctcgac gcctcggaac
    11041 atgacgaggt ctggattctg gccaacaacc accccgtagc gcgcggcgaa atcgagatcc
    11101 gcgaagaccg gatcgccatc acggtcaccc gtccggcgga tgtgtatgat ttcatggcgg
    11161 gcgctgctta ggcagcaggc agcaggcagc aggcagcagg cagcaggcag caggcagcag
    11221 gcagtagcgt attcacctcg atcgaggacg caaatgactt ctgatgtccg cagctatcgc
    11281 gatctgctcg tctggcaacg cgccatggat gttgcggtcg cggtctatga cctgtctcga
    11341 acatggcctc gcgacgaact gttcgggatg acgt
//
LOCUS       DASVTM010000311         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_615423,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000311 DASVTM010000000
VERSION     DASVTM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615423"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF633_08655"
     CDS             complement(<1..420)
                     /locus_tag="VF633_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471166.1"
                     /translation="MNSAAESSPQSSGADRWRLALQYVLLFGANGVSLPFAGLWFHAH
                     GLDGAEIGALFAAPMLGRLVTGPAIAIWADGFVYRRTAIAILALASAAAYAATGLVEG
                     FWLWAPLWFIAATAFGAIIPLSDVMTLRLARREGFSFS"
     gene            468..833
                     /locus_tag="VF633_08660"
     CDS             468..833
                     /locus_tag="VF633_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX8471167.1"
                     /translation="MILNRNLVVYAALNGAMAVMIGAFAAHGAGPAVKTLLTTGTHYQ
                     LVHAVLAVALAMWPNRLAGIAGWLAATGGLIFCLSLAFIGLLSMPAMGAVAPIGGVLM
                     IAGWLLLAFAALRNPSTNA"
     gene            863..>1740
                     /gene="pip"
                     /locus_tag="VF633_08665"
     CDS             863..>1740
                     /gene="pip"
                     /locus_tag="VF633_08665"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX8471168.1"
                     /translation="MAFTASEASRRDLYPEIEPFASGWMSTGSEHEIYYEECGNPQGK
                     PVIVLHGGPGGAVNPGMRRYFDPAKYRIVLFDQRGCGKSRPNASLEDNTTWTLIEDIE
                     RLREQCGIDKWAVFGGSWGSTLSLAYAITHPERVDSLILRGIFLLTKKELQWFYQDGA
                     SMIFPDAWERFVAPIPEAERGDLMAAYYKRLTGDDKAERERCAIAWSSWEGETVSVEG
                     PAAKPDKFAEPDFAVAFARIECWYFVNGGFFPSENWLLENISRIRHIPCWIAQGRFDV
                     VTPISGAWSLHRAWPEA"
BASE COUNT          305 a          606 c          543 g          286 t
ORIGIN      
        1 cgagaatgag aaaccctcac gccgcgccag cctcagggtc atcacgtcgc tgagcgggat
       61 gatcgccccg aacgccgtcg ccgcgatgaa ccacagcggg gcccacagcc agaagccttc
      121 gaccagccca gtcgccgcat aggccgccgc cgaggccagc gccagaatag cgatggccgt
      181 gcgccgatag acgaacccgt ctgcccagat agcgatggcc ggcccggtaa ccagccgtcc
      241 cagcataggc gcggcgaaca gggcgccgat ctcggctccg tccagtccat gggcatggaa
      301 ccacagcccc gcgaacggca ggctgacgcc gttggcgccg aacagcagca catactggag
      361 cgccaggcgc caccggtcgg cgcctgaaga ttgcggggac gactcggctg cggaattcat
      421 gcgtcccgat agcagacgac gagccgtccg tggggtagcg tctgtggatg attctgaatc
      481 gcaacctcgt cgtctacgcc gccttgaacg gagccatggc ggtcatgatc ggtgccttcg
      541 cggcgcacgg cgccggaccg gcggtcaaga ccctgctgac gaccggcacc cactatcagt
      601 tagtccacgc agtcctcgcc gtcgcactcg cgatgtggcc caatcgtctg gcgggcatcg
      661 ccggctggct ggcggcgacc ggcggtctga tcttctgcct gtccctcgcc ttcatcggtc
      721 tgttgagcat gcccgccatg ggagccgtcg cgccgatcgg cggggttctg atgattgcgg
      781 gttggctttt gctcgccttt gccgcattgc ggaacccgtc cacaaacgcc tgaacggcca
      841 gtctgaaagc tcttctcccc cgatggcctt caccgcatcc gaagcttcac gccgcgacct
      901 gtaccccgag atcgagcctt tcgcctccgg atggatgagc accggctccg agcacgaaat
      961 ctattacgaa gagtgcggca acccgcaggg taagcccgtc atcgtcctgc atggcggccc
     1021 cggcggggcg gtcaatccgg gcatgcgccg ctatttcgat ccggccaaat accgcatcgt
     1081 cctgttcgac cagcgcggct gcggcaagtc gcgccccaac gcctcgctcg aggacaacac
     1141 cacctggacc ctgatcgagg atatcgagcg cctgcgcgag caatgcggca tcgacaaatg
     1201 ggcggtgttc ggcggctcat ggggttcgac cctgtcgctg gcctacgcca tcacccaccc
     1261 cgagcgggtc gacagcctga tcctgcgcgg catcttcctg ctgacgaaga aggagctgca
     1321 atggttctac caggacggcg cctcgatgat cttcccggac gcctgggaac ggttcgtggc
     1381 ccccatcccc gaggccgagc gcggcgacct gatggctgcc tactacaaac gcctcaccgg
     1441 tgacgacaag gccgagcgcg agcgctgcgc catcgcctgg tcgagctggg aaggcgaaac
     1501 cgtcagcgtc gaaggtccgg ccgccaagcc ggacaagttc gccgagcccg atttcgcggt
     1561 ggccttcgcc cgcatcgaat gctggtattt cgtcaacggc ggcttcttcc cgtcggagaa
     1621 ctggctgctc gagaacataa gccgtatccg ccacatcccg tgctggatcg cgcaaggccg
     1681 cttcgacgtc gtgacgccga tctcgggcgc atggtcgctg catcgcgcct ggcccgaggc
//
LOCUS       DASVTM010000312         7159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_454957,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000312 DASVTM010000000
VERSION     DASVTM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454957"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..935
                     /locus_tag="VF633_08670"
     CDS             378..935
                     /locus_tag="VF633_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471169.1"
                     /translation="MLSSLLALPGSIIGAGSITLLYLKWRGDLQHPGVLAAAWAGIAA
                     SMVLWGAASKPDVGVPFAVGLMMLIALAFVLKGADLRALARFPDARRATSETAVRISW
                     PSAAARTLSAVVAAPVVGMTVGLLVWTHVAGHEANRFICAVVVFLAAFAALQVWGLSA
                     LRPWRALALIVLVGVAAAIPVALGL"
     gene            937..2457
                     /locus_tag="VF633_08675"
     CDS             937..2457
                     /locus_tag="VF633_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX8471170.1"
                     /translation="MARTLWPKASPGFVRRMLDGHKILGLALAGILYLICLTGWVGVY
                     YVEFERWERPNLPEFTVVTPEAAALAVADGRRFMLADAHRGPIDTDLYVGMPTQSMPR
                     LIAGYGHEARAYDDAGLYAGDASHDLTHFLTELHYYLHLPESFGMIIVCAFGVGMMAL
                     LIGGALSHPKMFRDAFSLRTRKQGQLGRVDVHNRLGVWTLPFAVAITLTGTMIGLAQL
                     VALALGVLAFDGDGLKAYEPIFGSQAEIDAATGGHPLQGDPAILTAIRHIRAAQPDQP
                     ISLIAIHQVGTPAESIEITTKPQQRLVYGETWRFNADGVLVGSHNLSDGPAGRQVAAS
                     VYSLHFGDFGGPLVKLIYAVLGLGLTVMIAAGMDIWLIKSAGKGRPRPALHRLWIALV
                     YGAPAAIALTFVVSMLTGINPVALFWWQMVALGMASLISMRFTAASARGMSRLMRLVL
                     GASTVAVPISHFLVHHSTTAATLQLSLPLLLIGLLMAASSVSGWLNLKVSRLVPAS"
     gene            2596..4701
                     /locus_tag="VF633_08680"
     CDS             2596..4701
                     /locus_tag="VF633_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX8471171.1"
                     /translation="MRRSLLLATASAALLGVAAAPAKAQSNGTQSNGAQSDESRVTDV
                     VVLGKRVIRESAGATGLDLSLRETPQSVTVVGAAQIKQFNLTDVNQLLATLPGINVEA
                     AETDRTYYNSRGFDIVNFQVDGVGQPLDWGLQTGALDIALYERVEAVRGANGLMTGTG
                     NPSATINYIRKRPTDEFQASASAGYGTWNNRRLTADVSGPLNEDGTVRARLIYANTDS
                     ESYLNYYGVNRNVYYGVVTWDVTPRLNATAGYSRQDNLATGVNWGALPLVYSNGTPIE
                     YDPSETTGAPWTYWDTHTDIGFGELSYAFDSGWTVKGIYTHRESTEDAKLLYAYGNPD
                     PATGLGVGGMSGIYPSSLKQNMFDVVTNGDFGLFGRRHQVVAGFNTSTNDSLKWEAFA
                     DYFDYPAYQGTHLVAPAEPAYPDSYLAEEIEDRLTRVYGAVHLNLTDALTAIVGVNAI
                     EVKTTGYSYGVSQDREENTVSPYVGVVYDVNRNVSLYASYSDLFAPQSESDINRIRLS
                     AAEGRSYEAGIKSEWLDKRLYATAAVFKSEQDGLAEYAGSIPGSLDNYYVGVDTIVEG
                     YEFEVSGRITPEWTVGGGFTDLSIEDGTGADTRLYVPRQTLKLNTTYTLPQYANLTLG
                     GAIRWQSDVSTEELGTTVTQDAYTVVDLLAAVDLTDHVRATLNAKNVFDEAYYGSLLW
                     SQAFYAPPRSVMVTLDYSF"
     gene            complement(4930..>7159)
                     /locus_tag="VF633_08685"
     CDS             complement(4930..>7159)
                     /locus_tag="VF633_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471172.1"
                     /translation="LSSFIGTGTYVPSATNLPTQAAVNGVFAGYGVAPGTVLNSRSLG
                     FNDSGTLFSQVGAVNYNGPTTGFWSTFGGTVRQPVTMQEYLVQPMERRNFFGKFEYDI
                     TDNISAYAQVLHVTSEVTGQVGWTPTVFVIPSMPVTNPFIPAGLRTILASRPNPLADF
                     TLNDRFVGFEDRKFRSDSKTDQYIVGLKGNLPIQDWTFDIYGLHDSVDIVETQDKALL
                     LSKMQTLFYAPDGGRSICAGGFNPFGLANGTAVSTACRNFIETETHDTTETEQDIVEG
                     TISGRMFALPAGDARFSLTGTYRSSSFEFDPDNARENNDIIGTLTTAPSQGSISVREA
                     AVEFLVPILADMPFAKSLDLSLGYRYSDYDVTGALSTYKIDGIWKPIETLSFRGGFER
                     AIRAPNIGELYSSALSAQVQIGTPPGGGDPCDIRSSARTTGGAAVRALCVATGVPAAI
                     VDSYQYTTVAIGSVASGSTSLTPEEADTLTVGAVWRPAVSNPWLEGLSLSVDYYDIEI
                     TNVISQVSGVTTMNKCYNLDGTNPTYSATNLYCQLITRDGTGGISTVATPYLNLGGLK
                     TSGFDIQADLRWNLADIGLGANAGELRINAVANFTNTYQVQLLPNTPWTEYAGTIDGT
                     QAATTPPVGLPLPDWKTFVNVTYSNGPAEIGFRWRHLPEMNDVTSVTRPASPAAGVPA
                     YDVVDLNLGWKLSETLALRGGVTNLFDEDPPIVGGTLGQTQPGTYDIIGRSYYVSLSA
                     RF"
BASE COUNT         1253 a         2230 c         2282 g         1394 t
ORIGIN      
        1 aggcggatga gcgcgcagac ggcatgcgga acgtgagtcg cgtctctcta tcgtccgacc
       61 ggacactccg atcacggccg cagggcccgg cgatttccgc cggatgcggg tctcatagcc
      121 gtgcttttca gggtagtcgc acagcgcctc gcggtcaccg gggccgtcat caccacaaga
      181 atacggaaca cctcgtcaat acggaacacc tcgtctcgcc gcctattccg ccggcgcccg
      241 tacatcgctg acggcgccag agacgcagcc accaaccgtc cttcaagtcg ctcgaccaat
      301 gggagcggat gatgatcaca attgcgaatg catcgcattt tccttgttgg agcgctgaat
      361 tggggtaaga ggcgcccatg ctctcgtccc ttcttgccct ccccgggtcg atcatcggcg
      421 ccggctccat tacgttgctg tatctgaaat ggcgcggcga ccttcagcat cccggcgttc
      481 tggccgcggc ctgggccggg atcgcggcgt cgatggttct gtggggcgcc gcctcaaagc
      541 ctgatgtggg tgtgccgttc gccgtcgggc tcatgatgct gatcgcgctg gctttcgtgt
      601 tgaagggcgc tgatctcaga gcgctggcgc ggttcccgga cgcccggcga gcgacgtccg
      661 aaaccgcggt gcggatctcc tggccttcag cggcggcccg gacgctgtca gctgtggtgg
      721 cggcgcccgt cgtgggcatg accgtgggtc tgctcgtctg gacccacgtg gcagggcatg
      781 aggcgaaccg gttcatctgc gcggtcgtgg tttttctcgc cgccttcgct gcgcttcagg
      841 tctggggcct cagcgccctc cggccgtggc gcgcgctggc tttgattgtt ctggtcggag
      901 tggcggccgc cattcccgtc gctctggggc tttagaatgg cgcgtacgct ctggcccaag
      961 gcttcacccg ggttcgtccg gcgcatgctg gacggtcaca agattctggg tctcgctctg
     1021 gcgggcatac tctacctgat ctgcctgacc ggctgggttg gggtttacta cgtcgaattc
     1081 gagcgctggg agcggcccaa cctgccggag ttcacggtcg tcacgcccga ggcggctgct
     1141 ctggccgtcg ccgatggccg acgtttcatg ctggccgacg cccacagagg gccgatcgat
     1201 accgatctct acgtgggaat gccgacccag agcatgccgc gcctgatcgc tggctatggg
     1261 catgaagcgc gcgcgtatga cgacgcgggc ctatacgccg gcgacgccag tcacgacctg
     1321 acccatttcc tgacggagct gcactactac ctgcacctgc ccgagagctt cggcatgatc
     1381 atcgtctgcg cctttggcgt ggggatgatg gcgctgctga ttggcggggc tctgtcgcat
     1441 ccgaagatgt tccgcgacgc cttttccctc aggacccgca aacagggtca gttggggcgg
     1501 gtggacgtgc acaaccggct gggtgtctgg accctgccat ttgctgtggc catcactctg
     1561 accggaacga tgattggtct ggcgcaactc gtggccctgg ccttgggagt actggcgttt
     1621 gatggcgatg ggctaaaggc ctacgagccg atcttcgggt ctcaggctga aatagacgca
     1681 gccaccggcg gtcaccccct tcagggcgat ccagcgatac tgactgcgat ccgccatatc
     1741 cgtgcggccc aaccggatca gcccatcagc cttatcgcca tccatcaggt cgggacgccg
     1801 gcggaatcaa tcgagatcac gaccaagcct cagcaaaggc tggtctacgg cgagacctgg
     1861 cggttcaacg cggacggcgt tctcgtgggc agccacaacc tgtcggacgg tcctgccgga
     1921 cgacaggttg ccgccagcgt ttacagcctg catttcggtg acttcggcgg tcccctggtc
     1981 aagctcatct atgccgttct gggattgggg ctgacggtga tgatcgccgc cgggatggac
     2041 atctggctga tcaagtcagc aggaaagggg cgtccccgcc ccgccctgca tcgcttgtgg
     2101 atcgcgctcg tctacggggc gcccgccgcc atcgccctca ccttcgtcgt gtccatgctg
     2161 acgggcatca accccgtggc cttgttctgg tggcagatgg tggccctcgg gatggcctcg
     2221 ttgatcagca tgcgtttcac cgctgcgtcc gcgagaggga tgtcgcgcct gatgcggctg
     2281 gttctgggcg cctcgaccgt cgctgtgccg atcagtcatt ttctggtcca tcactcgacc
     2341 acggcagcga cgctgcaact cagcctgccg cttttgctga tcggtctgct gatggcggcg
     2401 agttcggttt cgggttggct caatctgaag gtgtcccggc tggtcccggc ttcgtagcgg
     2461 ccgtcaggat ccagccggcg ccatttggcg cagtcgggct tggccatatt gacgcggcaa
     2521 aacttagagg ataatgcgag gcattctcaa gttggtgctt gaggcctaac gtcccctcat
     2581 cctgcgagat tccacatgcg tcgatccctc ctcctcgcca cggcttctgc tgccttgctg
     2641 ggcgtcgctg cggctcctgc caaagctcaa agcaacggga cccagagcaa cggcgctcag
     2701 agcgacgaaa gccgtgtcac cgacgttgtg gtgctgggca agcgcgtgat ccgtgaatca
     2761 gcgggcgcca ccggcctcga tctgtcgctg cgcgagacgc cccagtcggt caccgtggtc
     2821 ggcgcggccc agatcaagca gttcaatctc accgacgtga accagttgct ggccaccttg
     2881 ccgggcatca acgtcgaggc cgccgagacg gaccggacct attacaactc gcgcggcttc
     2941 gacatcgtga actttcaggt cgacggcgtc ggccagccgc tggactgggg gctgcagacc
     3001 ggcgctctcg acatcgcgct ctatgagcgg gtcgaagccg tgcgcggcgc gaacggcctc
     3061 atgactggca ccggcaaccc ctcggcgacg atcaactaca tccgtaaacg cccgacggac
     3121 gagtttcagg cctcggcttc ggcgggttac ggcacgtgga acaacaggcg tctgaccgcc
     3181 gatgtctccg ggccgctgaa cgaggacggc acggttcgcg ctcgcctgat ctatgccaat
     3241 accgacagcg agtcgtacct caactattat ggggtcaacc ggaacgtcta ctacggcgtc
     3301 gtcacttggg acgtgacgcc caggctgaat gcgacggcag gctattcccg acaggacaac
     3361 cttgcgaccg gcgtgaactg gggcgcgctg ccgctggtct attccaacgg cacgccgatc
     3421 gagtatgacc cgtccgaaac gaccggcgcg ccctggacct actgggatac gcacacagac
     3481 atcggttttg gcgagctgtc ctatgcgttc gacagcggct ggaccgtcaa gggcatctac
     3541 actcaccggg agtccaccga ggacgccaag ctgctgtacg cctacggcaa tcccgatcca
     3601 gcgaccggac tgggtgtcgg gggcatgagc ggcatctatc cctcctccct caagcagaac
     3661 atgttcgacg tggtcaccaa cggcgacttc ggcctgttcg gacgtcgcca tcaggtagtc
     3721 gcgggcttca atacctcgac gaatgacagt ctgaagtggg aggcgttcgc cgactatttc
     3781 gactatcccg cctatcaggg cacgcacctc gtcgcccccg ctgaaccggc ctatcctgac
     3841 tcctatctgg ccgaagagat cgaggaccgg ctgacccgcg tctacggcgc cgtccacctg
     3901 aacctcaccg acgccctgac cgccatcgtc ggcgtcaacg ccatcgaggt gaagacgacc
     3961 ggctattcct atggcgtgtc gcaggaccgc gaagagaaca cggtcagtcc ctacgttggc
     4021 gtcgtctatg acgtgaaccg caacgtctcc ctttacgcca gctattctga cctgttcgcg
     4081 ccgcagtccg agagcgatat caaccgcatc cgcctgtcgg ccgccgaggg ccgcagctac
     4141 gaggccggga tcaagagcga gtggctggac aagcggctct acgccactgc cgccgtgttc
     4201 aagtccgaac aggacggtct tgcagagtac gcaggctcca tcccgggctc gctcgacaac
     4261 tattacgtcg gcgtcgacac catcgtcgaa ggctatgagt tcgaagtttc gggccggatc
     4321 acgcccgaat ggaccgtagg cggtggcttt accgacctgt cgatcgagga tggaaccggc
     4381 gccgatacgc gcctgtatgt gccgcgccag accttgaagc tgaacactac ctatacgctg
     4441 cctcagtacg ccaatcttac gctgggcggc gctatccgct ggcagagcga cgtctcaacg
     4501 gaggagcttg gcacgacggt gacccaggac gcctacacgg tcgtggacct gctggccgcg
     4561 gtcgatctga ccgaccacgt ccgcgccacc ctcaacgcca agaacgtgtt tgacgaggcc
     4621 tattatggca gcctgctgtg gagccaggca ttctacgccc cgccccgtag cgtcatggtg
     4681 acgttggatt acagcttctg agatgcctga ggggcttggg gcggaggcag gccgcgcgct
     4741 tgtttagcgt caggcgcctt cgccctggct ttaccggggc gtggacaaca cggacgttgg
     4801 taccgcctgt gggttggacc ccgcagcgcc gctcggcgtg ttgaccggca ttcaggcacg
     4861 ttgcagcggc cgggaaaagg gccgcccgtg agggcggccc agggtcactg cgttgtcgag
     4921 acggctccgc tagaatcgag ctgacagcga tacgtaatag gatcggccga ttatgtcgta
     4981 ggtgcccggc tgcgtttgac cgagagtacc gccgacgatg ggcggatcct cgtcgaacag
     5041 gttggtgaca ccgccgcgaa gcgcgagtgt ttcgctcaac ttccaaccga ggttgaggtc
     5101 gacaacatca taggcgggaa cgcctgccgc cgggctggca gggcgggtga ccgaggtcac
     5161 atcattcatt tccggcaggt gacgccagcg gaaaccaatc tcggccggtc cgttgctgta
     5221 ggtcacgttg acgaacgttt tccagtcggg aagcggcaga ccgacaggag gcgtcgtcgc
     5281 ggcctgagtg ccgtcgatcg ttcccgcgta ttccgtccag ggcgtattgg gcagcaactg
     5341 cacctggtag gtgttggtga agttcgccac tgcattgatg cgcagttccc ccgcgttggc
     5401 tccaaggccg atgtcagcca ggttccaacg cagatcggcc tgaatatcga agccgcttgt
     5461 cttgagacct cccaggttga gatagggggt cgcaacagtc gagatgccgc cggtgccgtc
     5521 gcgggtgatc agttggcagt aaaggttcgt ggccgaatag gtcgggttcg tgccatcgag
     5581 attatagcat ttgttcatcg tggtaacgcc cgagacctgg gagatcacgt tcgtaatttc
     5641 gatgtcgtaa tagtcgacgg agagcgacaa accctcaagc cagggattgc tgacggcagg
     5701 ccgccaaacg gcgccgaccg ttagggtatc tgcttcttcc ggtgtcagcg aggtgctgcc
     5761 gctggcgact gatccgatgg ccaccgtcgt gtactgatac gaatcgacga tcgctgccgg
     5821 aacaccagtg gcgacgcaca gcgcgcggac ggctgccccg ccggtcgttc gcgcgctgga
     5881 gcggatgtcg cacggatcgc ctccgcctgg aggcgtaccg atctgcacct gggcggacag
     5941 ggcgctagag tacagttcac cgatgtttgg agcgcggatc gcgcgctcaa agccgcctcg
     6001 gaacgacaga gtttcgatgg gcttccagat gccgtcgatc ttgtaggtgc tcaacgcgcc
     6061 ggtgacgtca tagtcggaat accgatagcc cagcgataga tccaggctct tggcgaaggg
     6121 catatccgcc aggatcggca ccaggaactc gaccgccgct tcgcgcacgc tgatcgagcc
     6181 ctgagaaggc gcggtggtca gagtgccgat gatgtcgttg ttctcacgag cgttatcagg
     6241 atcgaactcg aagctgctgc tgcggtaggt ccccgtcagg gagaagcggg cgtcgccggc
     6301 cggcaaggcg aacatccggc cgctgatggt gccttccaca atgtcctgct cggtctccgt
     6361 ggtgtcgtga gtctcggtct cgatgaagtt gcgacatgcg gtggataccg ccgtgccgtt
     6421 ggccaggccg aacgggttga agcctccggc gcagatcgac ctgccgccat cgggcgcata
     6481 gaacagggtc tgcatcttgc tgagcaacaa ggccttgtcc tgggtctcca cgatatcgac
     6541 cgagtcgtgc aggccgtaga tgtcaaaggt ccagtcctga atcggcaggt tgcctttcag
     6601 cccgacgatg tactggtctg tcttcgagtc cgaccggaac ttgcggtctt caaagccgac
     6661 gaaacgatcg ttcagggtga agtcggccaa ggggttgggg cgggaggcga ggatggttcg
     6721 caggccggcg ggaatgaacg gattcgtcac cggcatcgac gggatcacga acaccgtcgg
     6781 tgtccacccc acctgacctg tcacctccga ggtgacatgg agcacctgag cataggcgga
     6841 gatgttgtcg gtaatatcgt actcgaattt tccgaagaaa ttccgccgct ccatgggttg
     6901 gaccagatac tcttgcatcg tcacaggctg ccgaaccgtg ccgccgaagg tgctccagaa
     6961 acccgtcgtc ggtccgttgt agttgaccgc gccgacctgg ctgaacagcg tcccgctgtc
     7021 gttgaagccg aggcttcttg aattcaggac cgtgcccggc gcgacgccat agcccgcgaa
     7081 cacgccgttc actgcagcct gggtcgggag attagtggcc gagggcacat aggtgccggt
     7141 tccgataaac gaagacagg
//
LOCUS       DASVTM010000313        10478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_511355,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000313 DASVTM010000000
VERSION     DASVTM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511355"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /gene="ybeY"
                     /locus_tag="VF633_08690"
     CDS             <1..196
                     /gene="ybeY"
                     /locus_tag="VF633_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX8471173.1"
                     /translation="CRDEAAAQGKSLTNHLTHLVIHGVLHVLGRDHEDDAEAEAMEAE
                     ERTLLASLGVADPDILPDDR"
     gene            234..1877
                     /gene="lnt"
                     /locus_tag="VF633_08695"
     CDS             234..1877
                     /gene="lnt"
                     /locus_tag="VF633_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX8471174.1"
                     /translation="MTSEPLADSTVHALAAGSDSRRRLVLRFGRIGLALLAGVGAAIA
                     HPPFGVLPGLLGYPLLMLLAERSEKVRGAFWMGWLGGFAYFFISCWWVAEAFMVDPSQ
                     AWMAPFAASLLPAGLGLFWGVALALYRRFAPAGVVRVLVFAALFALLEWLRGHVLTGF
                     PWNPAGASWAAGSAGSQFASVVGVYGLGFVTVAAVSAFAPLVGPGAREQRVAAALLGA
                     VALLALVIGGLVRLNGAKVEFTDTLVRIVQADVAQESKWTPEAYQGIVDRYVNLTAKL
                     GAALPDVVVWPEGALPASANDVFGPGSPDAGAIAGALKPGQTLLMGLARGEPGVSPGA
                     PDRYFNSLFALHDEGDVAGLRITAVYDKFRLVPFGEYLPLGNLMGSLGIRSLVHMPSD
                     FSAGPKPSPIDLPNAPRVQPLICYESLYPGFTQAGTHRPAWIVNVSNDAWFGRTSGPL
                     QHLNLASYRAIETGLPVVRATPTGVSAMIDPWGRLVPGKRLDPGESGVIDALLPRPAP
                     ATFYGRWGDLPFWLAVLGTLALGASWSRLRRPVEAAKNA"
     gene            2117..2542
                     /locus_tag="VF633_08700"
     CDS             2117..2542
                     /locus_tag="VF633_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8471175.1"
                     /translation="MARGAGEDGPHPVDRHVGRRVCEKRISLGYNQSDLGRALGLTFQ
                     QIQKYEKGANRISASKLWDIARFFKVEIGYFFQGLAQGQPGMAEDGVAAFDHDFPSTR
                     YSIEISRLAPQLSTRQQKLALDLIREMTDRPEDEAKAPA"
     gene            complement(2664..3194)
                     /locus_tag="VF633_08705"
     CDS             complement(2664..3194)
                     /locus_tag="VF633_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin family protein"
                     /protein_id="HEX8471176.1"
                     /translation="MSGLVKKTGVALVLISAALTMSGCDNGPSAVQTRDRADGAGAVL
                     TAAALGGPAEAASNNAGAPAEKLVVTANRRETASVKIARLYERNGAAFGARSAEDYAA
                     RMKGFLDNPPAGVDTVKRPNGDTLYYQASTNTFAVVSRDGTPRTMFKPDDGPTYWAQQ
                     KEGAPSFGKRTSQPTQ"
     gene            3313..4563
                     /gene="coaBC"
                     /locus_tag="VF633_08710"
     CDS             3313..4563
                     /gene="coaBC"
                     /locus_tag="VF633_08710"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX8471177.1"
                     /translation="MTNGALQSRKILLIVGGGIAAYKALELVRLIRKAGGEVKAVLTQ
                     AGAEFVTPLSLAALTGHPVRQTLFMPDDETSMGHIELSRWADLIVVAPATAGLIAKAA
                     NGLADDLASTTLIATDKKTLLAPAMNVRMWLHPAVQANVARLKGFDGFHGMAVVGPDD
                     GEMACGEFGPGRMAEPAIILQTIIELLAGPDGRPLLGRKAIVTAGPTFEAIDPVRGLT
                     NRSSGKQGYAIAASLAALGAEVTLVSGPTGLPAPVGVTRIDVESALDMKAATEAALPA
                     DIAVLSAAVADWRVDTIAGGKIKKGPGGPPTLALIENPDILAGIASPGPLRPTLVIGF
                     AAETTDLEANARTKLSRKGCDWIVGNDVSDDVFGSDGNAVLLVTREATETWPRQPKTA
                     IARALAARIADHFETLPDIGGKPE"
     gene            4560..5009
                     /gene="dut"
                     /locus_tag="VF633_08715"
     CDS             4560..5009
                     /gene="dut"
                     /locus_tag="VF633_08715"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX8471178.1"
                     /translation="MTTLRIQRLPHSEGLALPAYETTGSAGMDLRAALPAAAPMTLAP
                     GARALVPTGLKIALEQGWEAQVRPRSGLALKHGVTCLNSPGTIDSDYRGEVGVILINH
                     GAEPFVIQRGERIAQMVIAAHAQAVIAEVDGLDETARGEGGFGSTGR"
     gene            complement(5012..5950)
                     /locus_tag="VF633_08720"
     CDS             complement(5012..5950)
                     /locus_tag="VF633_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8471179.1"
                     /translation="MSGQAGANTDQIASGIALRIASAGCFSIMSALLKVASTHGVVAT
                     EMLFYRALFGLPVVVLWVMTSQGGFKALRTERPWAHVRRSVLGISGILCIFQALTMLP
                     LADATTLGFTAPIFATLLSAVLLHETVGKHRWIAVLIGFAGVAVVMRPGSEPGSAHSL
                     PLLGLAIGLMGAVLQAGVNVTLRQLKDTEHVAAIVFWFFVASLVVGGLVLPVFGHWHG
                     WTTLALLAGGGVAGGLAQLTVTASLRAAPVAVVAPFDYLQIVGAVVFGWWLLASPPSL
                     NTLAGAALIASSGLYTVWREHRRHRDRLMQPSTPPV"
     gene            6104..6469
                     /locus_tag="VF633_08725"
     CDS             6104..6469
                     /locus_tag="VF633_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471180.1"
                     /translation="MENFFDQIGELFSGLFALAQGGFDGVNQVVGLVIALIAAMMMPS
                     WRSLWGTAVGAVIVDRLILMLRPALDGGSIALPNIMTGSFWMTAFALFLGYSIVIAIF
                     FLLKTILTGTAFRGRARAH"
     gene            complement(6611..7360)
                     /gene="dapB"
                     /locus_tag="VF633_08730"
     CDS             complement(6611..7360)
                     /gene="dapB"
                     /locus_tag="VF633_08730"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX8471181.1"
                     /translation="MSGIFHAGISGARGRMGRAVDQVLDEREDVMVAVRFDWGETPDL
                     SLCDVVIDFSTAEASVTLAQLAAERGGPALVIGSTGFTPEQDAEIMKAAEKVAIVRSG
                     NYSLGVNILIGLVEHAALRLDAQDWDIEVIEAHHRLKVDAPSGTALMLGEAAAAGRGH
                     DLDEVRLKPYDGITGEREPGKIGFASIRAGGIIGEHTVLFASKDETLTLSHSAIDRSL
                     FAKGAVAAAAWVRTRRPGLYDMQDVLGFRQA"
     gene            complement(7368..8570)
                     /gene="dnaJ"
                     /locus_tag="VF633_08735"
     CDS             complement(7368..8570)
                     /gene="dnaJ"
                     /locus_tag="VF633_08735"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8471182.1"
                     /translation="MARDYYEVLEVERTIDAAGLKSSYRKLAMIHHPDRNGGSEESMA
                     KFKEISEAYTVLSDDQKRAAYDRFGHAGVNGGAAGGGAQQGFHDVNDIFSQVFGDAFG
                     DVFGGRGGRQQNNGPRRGSDLRYDLEITLEQAYQGAEVEVSIPSTLTCDTCEGTGAKK
                     GSKPTTCSTCAGQGRVRQANGFFQVERTCPSCGGAGQKISAADVCTTCHGHGQVRKTR
                     NLNLKVPAGVDDGSRIRLSGEGDAGQRGGPRGDLYVFLSVQPHELFERDNLDLLCTVP
                     VPMTVAALGGEIDAPCLVSTACDGECKAPVTVPAGAQTGKTVRIKGKGMPHLNGRQRG
                     DLVVELFVETPTDLTARQKELMQELALSFGESQNPRNTSFAGKAKRFWADILGADEAE
                     KPSRETAQ"
     gene            complement(8754..>10478)
                     /gene="dnaK"
                     /locus_tag="VF633_08740"
     CDS             complement(8754..>10478)
                     /gene="dnaK"
                     /locus_tag="VF633_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8471183.1"
                     /translation="PTNTFFAIKRLIGRSFDDPVVAKDKNMVPYEIVKGPNGDAWVRA
                     NGKDYSPQQISAFTLIKMKEAAEAYLGEKVTQAVITVPAYFNDSQRQATKDAGKIAGL
                     EVLRIINEPTAAALAYGLDKNEGKKIAVYDLGGGTFDVSILEIGDGVFEVKSTNGDTF
                     LGGEDFDLRLVDYLADEFKKEQSVDLRSDKLALQRLKEEAEKAKKELSFTSSYEVNLP
                     FITMNASGPLHLNIKLSRAKLEALVDDLIQRTIEPCAKALKDAGLKASDIDEVVMVGG
                     MTRMPKVVEAVKAFFGREPHTGVNPDEVVALGAAVQAGVLQGDVKDVLLLDVTPLTLG
                     IETLGGVFTPLIERNTTIPTKKSQVFSTADDNQSAVTIRVFQGERPMASDNKVLGQFD
                     LMGIPPAPRGLPQIEVAFDIDANGIVHVTAKDKATNKEQSIRIQANGGLSDADIDKMV
                     KEAEANATADKAKKDLVEANNAAESLIHSTEKALADHGDKVGSDIKDSIQTALDELKA
                     AKEEGTNPEDIRAKTNTLVQASMKLGEAMYAQGQASDEDVTATADDGVVDAEFEEVDG
                     DEDEKKSA"
BASE COUNT         1715 a         3444 c         3391 g         1928 t
ORIGIN      
        1 ctgtcgtgac gaggcggctg cgcagggcaa gtcactgacc aaccatctga ctcatctggt
       61 catccatggc gttctgcacg tacttgggcg cgaccatgag gacgacgccg aggccgaggc
      121 gatggaggcg gaagaacgca cccttcttgc cagcctgggc gtcgccgacc cagatatcct
      181 gcctgacgac cgctgaacgt cccgatctcc gggccgcatg atctccgaac tccatgactt
      241 ctgaacccct cgccgactcc accgtccatg cgctcgctgc ggggagcgac agccgtcgtc
      301 ggctggtgct gaggttcggc cgtatcgggt tggcgctgct ggcaggcgtc ggcgcagcca
      361 tcgcccatcc cccgttcggt gtcctgccgg gcctgctcgg ctatcccctg ctgatgctgc
      421 tggccgagcg gtcggagaag gtgcgcggcg ccttctggat gggctggctc ggcggattcg
      481 cctatttctt catttcctgc tggtgggtgg cggaggcctt tatggtcgat ccgtcgcagg
      541 cctggatggc gccgttcgca gccagtcttc tgcctgccgg gctagggcta ttctggggcg
      601 tcgcgctcgc cctctatcgc cggttcgcac ccgccggagt cgtccgggtt ctcgtcttcg
      661 ccgccctgtt cgccctgctt gaatggctac gcggccatgt cctgacgggc tttccgtgga
      721 accccgccgg ggccagttgg gccgctggat ctgccgggtc gcagttcgcg tccgtcgtgg
      781 gcgtctacgg gctgggcttc gtcacagtcg ccgcagtctc ggccttcgca ccgctggtgg
      841 gaccgggggc gcgcgagcaa cgcgtagccg ccgccttgct cggcgccgtc gcgctgcttg
      901 ccctcgtcat cggcggctta gtccgattga acggcgccaa ggtcgagttt accgacaccc
      961 tcgttcgtat cgttcaggcg gacgtggcgc aggaatccaa gtggacgccc gaggcctatc
     1021 aggggatcgt cgaccgctac gtcaacctga cggccaagct cggcgctgcg cttcccgacg
     1081 tcgtggtctg gcccgagggc gcactgcctg ccagcgccaa cgacgtattc ggcccgggtt
     1141 cgcctgacgc cggcgccatt gcaggcgccc taaagccggg ccaaaccctg ctgatgggtc
     1201 tcgcgcgggg cgagcctgga gtgtctcccg gagcgcccga ccgctacttc aacagcctgt
     1261 tcgcgctgca tgacgagggc gacgtcgcgg gcctgaggat caccgctgtc tacgacaagt
     1321 tccggctggt gccgttcgga gagtacctgc cgctaggaaa tctgatggga tcactgggca
     1381 tccgcagcct tgttcacatg ccgtccgact tcagcgcggg ccccaagccc tcaccgatcg
     1441 acctgcccaa tgcgccgaga gtgcagccgc tgatttgcta tgaaagcctc tatcccgggt
     1501 ttacccaagc aggaacccac cggcccgcgt ggatcgtcaa tgtctcgaat gatgcctggt
     1561 tcgggcgaac gtcagggccg ctgcaacacc tgaacctcgc cagctatcgc gccatcgaga
     1621 cgggtctgcc ggtcgtgcgg gcgaccccga ccggagtgtc ggcgatgatc gatccatggg
     1681 gccgtctggt gccgggcaag cggttggatc cgggtgagag cggagtcata gacgccttgc
     1741 tccctcgccc ggcaccggcg accttttacg gacgatgggg cgacctgcct ttctggctgg
     1801 ccgtactggg gacccttgcc cttggagctt catggtcgcg tcttcgtcgt ccggtagagg
     1861 ccgcgaagaa tgcctgatac cggtggacta aaaacgcgcg cctagccgca aaccctgcat
     1921 ctctggtctg gtacgaaacg tacctgaccc gtgttcgctt gaatttggtt cttttgtctg
     1981 cgaaatcagc agaaactggt gtctaacggt tgcaacagaa cccgaagcgt cgcggtatgt
     2041 gtattattgg atgacagccg ctgaaacgcg gcgcatcagc aggatcgcgt gagtacgcgc
     2101 agacggcgag agaaatatgg ctagaggcgc aggcgaagac ggaccccatc ctgtcgaccg
     2161 tcacgtaggc agacgcgttt gcgaaaaacg tatcagcctc ggatataatc agagcgatct
     2221 aggccgggct ctcgggctga cgttccagca aatccagaaa tacgagaagg gcgccaaccg
     2281 catctcggcc tccaagcttt gggacatcgc gcggttcttc aaggtcgaga tcggctactt
     2341 tttccagggc ctcgcccagg gacaacccgg catggccgag gacggcgtcg ccgccttcga
     2401 tcacgacttt ccgtcgaccc gctattcgat cgagatctct cgtctggccc cacagctgtc
     2461 gacccggcag cagaagctgg cgctcgacct gatccgcgaa atgacggatc gcccggaaga
     2521 cgaggccaag gctccggcct gaggcggatc acaacggcgc cccgtcgggt ccgtgtgtgc
     2581 gacccgcatc gaacctgccg gattttaagc gccgccgtcg caatgcgaac ccctgacccc
     2641 gtccgcgtaa ggacgagggt ctatcactgt gtcggctggc ttgtgcgctt cccgaagctt
     2701 ggggcgccct ctttctgttg cgcccagtat gtagggccgt catccggttt gaacatcgtt
     2761 cgcggcgtgc cgtcgcgcga cacgaccgcg aaggtgttgg tggacgcctg ataatagagg
     2821 gtatcgccgt tgggccgttt gaccgtgtcc acgcccgccg gcggattatc gaggaacccc
     2881 ttcatccgcg ccgcatagtc ttccgccgag cgcgcaccga aagcggcgcc gttgcgctcg
     2941 tacaatcggg cgatcttgac gctcgccgtc tctcgccggt tggcggtgac gacaagtttc
     3001 tcagctgggg cgccggcatt gttcgaggcc gcttccgccg ggccaccgag cgccgctgct
     3061 gtcaaaaccg ccccggctcc gtccgcccga tctcgtgtct gcaccgcaga gggcccgttg
     3121 tcgcaccccg acatcgtcag cgccgccgag ataagaacca atgcgacgcc cgtcttctta
     3181 accagaccgc tcatccaacc ctccatcgaa ctcagtgaac gcagttacgc ataggttgaa
     3241 cgttcctgca atgttctttt cttaagcggg catcgtctcg acgtgccgcg cctgcgacgc
     3301 tagagacagg ccatgaccaa cggcgctctc cagtcccgca agatcctgct gatcgtcggc
     3361 ggcggcatcg cggcctacaa ggcgctcgaa ctggtgcggc tgatccgaaa agccgggggc
     3421 gaggtcaagg ccgtgctgac gcaggccggc gccgagttcg taacgcccct gtcgctggca
     3481 gccctgaccg gccacccggt gcgccagacc ctgttcatgc ccgacgacga gacatcgatg
     3541 ggccatatcg agctgtcgcg ctgggctgac ctgatcgtgg tcgcgccagc aaccgccgga
     3601 ttgatcgcca aggcagccaa cggattggcc gacgacctcg cctcgaccac actgatcgcc
     3661 acagacaaga agaccctgct ggcgcccgcc atgaacgtgc gcatgtggct gcatccggcc
     3721 gttcaggcca atgtcgcacg gctcaagggc ttcgacggtt tccacggcat ggcggtcgtc
     3781 ggcccagacg acggcgagat ggcctgtggc gagttcggcc ccggtcggat ggccgaacct
     3841 gcaatcatcc tccaaacgat catcgagctg ctggccggtc ccgatggacg cccgctgctg
     3901 ggccgcaagg ccatcgtgac ggcaggcccg accttcgagg ccatcgaccc tgtgcgcggc
     3961 ttgaccaacc gctccagcgg caagcagggc tacgctatcg ccgcatccct cgcggccctc
     4021 ggcgccgagg tgacgctggt gtccggcccg accggtcttc ccgcgcccgt cggcgtgacc
     4081 cgcatcgatg tcgagagtgc gctggacatg aaggccgcga ccgaggccgc gttgcctgcg
     4141 gacatcgccg tgctgtcggc cgccgtcgct gactggcgtg tcgacaccat cgccggcggc
     4201 aaaatcaaga agggtcccgg cggaccgccg acgctggcct tgatcgagaa cccggacatc
     4261 cttgcgggga tcgcctcccc cggtccgctg cggccaacgc tggtgatcgg tttcgccgcc
     4321 gagaccacag acctcgaggc caacgcgcgg accaagctat cgcgcaaggg ctgcgactgg
     4381 atcgtcggca atgacgtctc agacgacgtc ttcggctcgg acggcaatgc ggtgttgctg
     4441 gtcacccgcg aggcaaccga gacctggccc cgccaaccca agacggccat cgcccgagcc
     4501 ctcgccgccc gcatcgccga tcatttcgag acccttcctg acattggcgg aaagcctgaa
     4561 tgaccactct gcgtatccag cgcctgcccc actccgaggg actggcgctg ccggcctacg
     4621 agacaaccgg atccgcaggc atggacctgc gcgccgcctt gccggccgcc gctccgatga
     4681 cgctcgcacc gggcgctcga gctctggtcc cgacagggct gaagatcgcg ctggagcagg
     4741 gctgggaggc gcaagtccgg ccgcgctcag gtctggcgct caaacatggc gtcacctgcc
     4801 tcaactcgcc cggcacgatc gacagcgact atcgcggcga ggtcggggtc atcctgatca
     4861 accacggcgc cgagcctttc gtgatccagc gcggcgaacg catcgcccag atggtcatcg
     4921 cggcccacgc acaggccgtg atcgcagagg tcgacggcct cgacgaaacc gcgcgcggcg
     4981 aaggcggctt cggctctacg gggcggtagt ttcagaccgg cggcgtgctc ggctgcatca
     5041 ggcggtcgcg gtgacgccgg tgctcgcgcc agacggtgta gaggccgctg gaggcgatga
     5101 gggcggcgcc ggcgagggtg ttcaggcttg gcggactggc cagcagccac cagccgaaga
     5161 cgaccgcgcc gacgatctgc agatagtcga acggcgccac caccgcgacc ggggcggcgc
     5221 gcagtgaggc ggtcacggtc aactgcgcca gaccgcccgc cacgcctcca ccggccagca
     5281 aggccagcgt cgtccagccg tgccagtggc cgaagacagg gagcacgagg cctccgacca
     5341 cgagcgaggc gacgaagaac cagaagacga tggccgcgac atgctcggtg tccttgagtt
     5401 ggcggagggt gacgttgacc ccggcttgca gcaccgcacc catgagaccg atcgctaggc
     5461 ccagcagcgg cagggaatgg gccgagcccg gctccgaacc cggccgcatc accacggcca
     5521 cgcccgcaaa gccgatgagg actgcgatcc accggtgctt gccgaccgtc tcgtgcagaa
     5581 gcacggccga caggagggtg gcgaagatgg gtgccgtgaa ccccagcgtc gtggcgtccg
     5641 ccagcggcag catggtcagg gcctgaaaga tgcagaggat gcccgagatg cccaagacgc
     5701 ttcggcggac gtgggcccag ggccgctccg tcctcaaggc cttaaacccg ccctgcgatg
     5761 tcatgaccca caggacgacg accggcagac cgaacagggc gcgatagaac agcatctcgg
     5821 tcgcgacgac gccgtgggtc gacgccacct tcaacagcgc agacatgatc gagaaacagc
     5881 ccgccgaggc aatccgcagc gcaatacccg aagcgatctg atcggtgttc gcgcctgcct
     5941 gtccgctcat gccattgacg ccgtcttccg catggcgcgc tgtgtagcga accgagcagg
     6001 ggatgtccgc tcaaaccggc tgatcgccgg atggagctaa tcccgccgac cggcgttaac
     6061 cctgaagcta acggccaagc tggcctcatc aggagcgaac gtgatggaaa acttcttcga
     6121 tcagatcggc gagctgttca gtggtttgtt cgccttggcc caaggcggct tcgatggcgt
     6181 caatcaggtg gtggggctgg tcatcgccct gatcgcagca atgatgatgc cgtcgtggcg
     6241 cagtctgtgg ggcacggctg tgggcgccgt catcgtcgat cggttgatcc tgatgctgcg
     6301 gcccgcgctg gacggcggtt cgatcgcgct gccgaacatc atgaccgggt ctttctggat
     6361 gacggccttc gccctgttcc tcggctattc catcgtcatc gcgatcttct tcctgctgaa
     6421 gacgatcctg acggggactg cattccgtgg tcgcgcccga gcgcactaag cttgggtcgc
     6481 ttcgcgacgc gctacgttcg gttcgcggag cctcactgct tgagcgcgaa gatattcctg
     6541 ccttagcttg cgctcaagta gtgagcgacc gcgtcgcgaa cgtagcgcgg cgcgccagcg
     6601 gctcgacagg ctaagcctgt ctaaaaccaa gaacgtcctg catgtcgtag aggcccggtc
     6661 ggcgggtgcg gacccaggcg gcggcggcga cggcgccctt ggcgaacagg ctgcggtcga
     6721 tggccgagtg cgacagggtc agggtctcgt ccttggacgc gaacaagacc gtatgctcgc
     6781 cgatgatccc gccggcccgg atcgaggcga agccgatctt gccaggctcg cgctcgccgg
     6841 tgatgccgtc gtaaggcttc aggcggacct catcgagatc gtgcccacga cctgcggcag
     6901 cagcctcgcc cagcatcagg gccgtgcccg agggggcgtc gaccttgagc cggtgatgag
     6961 cctcgatgac ctcgatgtcc cagtcctgcg cgtccagtcg cagggcggcg tgttcgacca
     7021 gcccgatcag gatgttgacg cccagcgagt agttgccgct gcggacgatg gcgaccttct
     7081 cggccgcctt catgatctcg gcgtcctgtt ccggggtgaa gcccgttgag ccgatcacca
     7141 gagcgggtcc gccgcgctcc gccgccagtt gtgccagcgt caccgaggct tcggcggtcg
     7201 agaagtcgat gaccacgtcg cacaggctca gatcgggcgt ctcgccccag tcgaagcgca
     7261 ccgccaccat gacgtcctcg cgctcatcga gcacctgatc gactgcgcgg cccatccggc
     7321 cacgggcgcc ggagataccg gcgtggaaga tgccgctcac tgaaagctca ttgggcggtt
     7381 tccctggaag gcttctcggc ctcgtccgcg ccgaggatat cggcccagaa acgcttggcc
     7441 ttgcccgcga acgacgtgtt gcgcggattc tggctctcgc cgaacgacag cgcaagctcc
     7501 tgcatcagct ctttctggcg cgcggtcagg tcggtcgggg tctcgacgaa cagttcgacc
     7561 accagatcgc cgcgctggcg accgttcagg tgcggcatgc ccttgccttt gatacggacg
     7621 gtcttgccgg tctgggcccc ggccggaacc gtcacaggcg ccttgcactc gccgtcgcag
     7681 gcggtggaca ccagacaggg cgcgtcgatc tcaccgccca aggccgcgac cgtcattggc
     7741 acgggaacgg tgcacagcag gtcgagattg tcgcgctcga acagctcgtg cggctggacc
     7801 gacaggaaga catacaggtc gccgcgcgga ccgccgcgct ggccggcgtc gccctcgccc
     7861 gacaggcgga tgcgcgagcc gtcgtcaacg ccagccggaa ccttgaggtt caggttgcgg
     7921 gtcttacgga cctgaccgtg accgtggcag gtggtgcaga cgtccgccgc cgaaatcttc
     7981 tggccggcgc caccgcagct cgggcaggtg cgctcgacct ggaagaaacc gttggcctga
     8041 cgcacgcggc cctgaccggc gcaggtcgaa caggtggtag gcttggaacc ctttttggcc
     8101 cccgtgcctt cgcaggtgtc gcaggtcagg gtcgagggga tcgagacctc gacctcggcg
     8161 ccctgatagg cctgctccag cgtgatctcg aggtcgtagc gcaggtccga accccggcgc
     8221 ggtccgttgt tttgctgacg tccgccgcga ccaccgaaaa cgtcgccgaa tgcgtcgccg
     8281 aacacctgcg agaagatgtc gttgacgtcg tggaagccct gctgagcacc gccgccggcc
     8341 gcgccgccgt tgacgcccgc atgaccgaac cggtcatagg cagcgcgctt ctgatcgtcc
     8401 gacagcaccg tataggcttc ggagatttcc ttgaacttgg ccatggactc ttccgagccg
     8461 ccgttgcggt ccgggtggtg gatcatggcc agtttgcgat agctggattt caggcctgcg
     8521 gcgtcgatag tccgctcgac ttccagaact tcgtaatagt cacgcgccat ctggcgaact
     8581 cgtcctctat ccctgtcgcc ccggcggcgt ctctatggac gatcggcgcg gggcggtgac
     8641 ccaaccttgt ctataagaca gatggtatgc gggtcgtgtg atgcaagctt cataaaagaa
     8701 ggccccgctg ccgtctgaca agcggggcct tcgggatata tcgagcgtat tgcttacgcg
     8761 ctcttcttct cgtcttcgtc gccgtcgact tcctcgaact cggcgtcgac aacgccgtcg
     8821 tcggcagtgg cggtgacgtc ttcgtcggaa gcctgaccct gagcgtacat ggcttcaccc
     8881 agcttcatcg aggcctggac cagagtgttg gtcttggcgc ggatgtcttc ggggttcgtg
     8941 ccttcttcct tggcggcctt gagctcgtcg agggcagtct ggatgctgtc cttgatgtcc
     9001 gagccgacct tgtcgccgtg gtccgccaga gccttctcgg tcgagtggat caggctttcg
     9061 gcggcgttgt tcgcctcgac cagatccttc ttggccttgt ccgccgtggc gttggcttcg
     9121 gcttccttga ccatcttgtc gatgtcggcg tccgagaggc cgccgttcgc ctgaatgcgg
     9181 atcgactgtt ccttgttggt cgccttgtcc ttggccgtta cgtgcacgat gccgttggcg
     9241 tcgatgtcga aggcgacctc gatctgcggc aggccgcgcg gagccggcgg aatgcccatc
     9301 aggtcgaact gacccagcac cttgttgtcg gacgccatcg gacgctcgcc ctggaagacc
     9361 cggatggtca cggccgactg gttgtcgtcg gcggtggaga acacctggct cttcttggtc
     9421 gggatcgtgg tgttgcgttc gatcagcggc gtgaacacgc cacccagcgt ctcgatgccc
     9481 agcgtcagcg gggtcacgtc gagcagcagc acgtccttga cgtcgccttg cagaacgccg
     9541 gcctgaacgg cggcgccgag agcgacgact tcgtccgggt tcacgcccgt gtggggttca
     9601 cgaccgaaga aggccttaac cgcctcgacg accttgggca tgcgggtcat gccgccgacc
     9661 atcacgactt catcgatgtc cgaggccttg aggcccgcgt ccttgagcgc cttggcgcag
     9721 ggctcgatcg tgcgctggat caggtcatcg accagagctt ccagcttggc gcgcgacagc
     9781 ttgatgttca ggtgcagcgg acccgaggcg ttcatggtga tgaagggcag gttcacttcg
     9841 taggatgagg tgaacgacag ttccttcttg gccttttcgg cctcttcctt caggcgctgc
     9901 agggccagtt tgtcggaccg aaggtcgacg ctctgttcct tcttgaactc gtcggcgagg
     9961 taatcgacca gacgcaggtc gaagtcttca ccgccgagga aggtgtcgcc gttggtcgac
    10021 ttcacttcga acacgccatc gccgatctcg aggatcgaga cgtcgaaggt gccgccgccg
    10081 aggtcgtaga ccgcgatctt cttgccttcg ttcttgtcca gaccataggc cagggccgct
    10141 gcggtaggct cgttgatgat gcgcaggact tcgaggccgg cgatcttgcc tgcgtccttg
    10201 gtggcctggc gctggctgtc gttgaagtag gcgggaacgg tgatgacggc ttgcgtcacc
    10261 ttttcgccga ggtaggcctc agcagcttcc ttcatcttga tgagggtgaa ggccgaaatc
    10321 tgttgcggcg agtagtcctt gccgttggcg cgcacccagg cgtcgccgtt cgggcccttg
    10381 acgatctcgt aagggaccat gttcttgtcc ttcgcgacca ccgggtcgtc gaagctacgg
    10441 ccaatcaaac gcttgatggc gaagaaggtg ttggtcgg
//
LOCUS       DASVTM010000314         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1040890,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000314 DASVTM010000000
VERSION     DASVTM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040890"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1027)
                     /gene="lipA"
                     /locus_tag="VF633_08745"
     CDS             complement(32..1027)
                     /gene="lipA"
                     /locus_tag="VF633_08745"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX8471184.1"
                     /translation="MVTLIDTLKISPAALRHPEKQNRPESAILKKPDWLRVKAPGSGQ
                     YNATREIVRSKGLVTVCEEAACPNIGECWSQKHATLMIMGDTCTRACAFCNVKTGMPQ
                     PLDPEEAGKVGLAVQQMGLNHVVITSVDRDDVSVGGAAHFAEVVRQIRIQAPNTTIEI
                     LTPDFLRKDGAAAVMIDARPDVFNHNLETVPRLYLKIRPGARYFHSLRLLQMVKERDP
                     NQFTKSGIMVGLGETKEEVMQVMDDMRSAGVDFITIGQYLQPTRKHAAIERFVTPEEF
                     KAYEAIARAKGFLMVSASPLTRSSHHAGDDFRRLRAARDAKLAGGRLTLTGAPTA"
     gene            1252..>2483
                     /locus_tag="VF633_08750"
     CDS             1252..>2483
                     /locus_tag="VF633_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8471185.1"
                     /translation="MPLAPNSDAFPATLYEEPLFDALISARAKYGDKEILEDQDRKPL
                     TYTGLIRAAFVLGRKIAVMTQPGERVGILLPSSMGVVVTFFGLHAFGRVPVMLNFTAG
                     EANLKAALATSGVKRILSAKRFIQQAKLEDLTDELAKVAEITWLDDVRATIGLADKLF
                     GLAAGMAPRRFRTKTDADGPGVVLFTSGSFGAPKGVVLSQRNLVSNARQISAHIAMDA
                     DWVMFNPLPTFHSLGLTGGVILPLLQGMKAFEYPSPLHAKQITDLLPKVGASILFATD
                     TFLNQYGRVGEPEDFATLKFVVAGAEKVRDETRHLFNTRFGGVTLLEGYGATEAAPVI
                     AVNHPDRNRPGSVGQVLPGIEYRLEDVPGITGGGQLYVRGPNVMEGYISPDDPLGLDM
                     LHEGWHDTGDIVDIDADG"
BASE COUNT          386 a          795 c          857 g          445 t
ORIGIN      
        1 gtgtacggca ggacgtgccg gaactcgcgt ctcacgcggt gggggcgccg gtcagggtga
       61 ggcggccgcc ggccagcttg gcgtcgcggg cggcgcgcag ccggcggaag tcgtcgccgg
      121 cgtggtgcga ggaccgcgtc agcgggctgg cggagaccat caggaagccc ttggcccggg
      181 cgatcgcctc ataggccttg aactcttccg gggtgacgaa ccgctcgatg gccgcgtgct
      241 tgcgggtcgg ctggaggtac tggccgatgg tgatgaagtc gacgccggcg gagcgcatgt
      301 cgtccatcac ctgcatgacc tcttccttgg tctcgccgag accgaccatg atgccggact
      361 tggtgaactg gttcgggtcg cgctccttga ccatctgcag caggcgcagc gagtggaagt
      421 agcgggcgcc gggacgaatc ttgagataga gccgcggcac ggtttcgagg ttgtggttga
      481 acacgtccgg gcgggcgtcg atcatcaccg cagccgcgcc gtccttgcgc aggaagtcgg
      541 gcgtcaggat ctcgatggtg gtgttcgggg cctggatgcg gatctggcgg accacttcgg
      601 cgaagtgggc ggcgccgccg accgatacgt catcgcggtc gaccgaggtg atgacgacgt
      661 ggttcagacc catctgctgc acggcgaggc cgaccttgcc ggcttcttcg gggtcgagcg
      721 gctggggcat gccggtcttg acgttgcaga aggcgcaggc ccgcgtgcag gtgtcgccca
      781 tgatcatcag cgtggcgtgc ttctggctcc agcactcgcc gatgttggga caggcggcct
      841 cttcgcagac ggtcaccagc cccttggagc ggacgatctc gcgcgtggcg ttgtactggc
      901 ccgagccggg cgccttgacc ctgagccagt cgggtttctt caggatcgcc gactccggcc
      961 gattctgctt ctccgggtga cgcagggcgg ccggggatat cttcagggta tcgatcagcg
     1021 tgaccatagc gggcggtcgg gctccttcgg ggggaacagt ccggcgggga taggtcggta
     1081 tgtcgtcttt ccgggcccgg aaggcaagtc gggcgccccg cacgttgggt ttcagcatcg
     1141 catcggaaca gcctcacgca acgtaactta tcttttcaag ccgggctgac ggctttaggg
     1201 tccgccgcct gatgcgaggg ggtcgttcca gccccccgga ggagattacg cttgccgctc
     1261 gccccgaact ccgacgcctt tcctgcgacg ctctacgaag agccgctgtt cgacgccctg
     1321 atctcggcgc gggccaagta cggcgacaag gagatcctgg aggatcagga ccgcaagccc
     1381 cttacctaca ccggcctgat ccgcgcggcc ttcgtgctgg gtcgcaagat cgcggtcatg
     1441 acccagccgg gtgagcgggt cggcatcctg ttgccgtcat ccatgggtgt ggtcgtcacc
     1501 ttcttcggcc tgcacgcctt tggacgcgtg ccggtgatgc tgaacttcac cgccggcgag
     1561 gccaacctga aggcggctct ggcgacgtcg ggggtcaagc gcatcctttc ggccaaacgc
     1621 ttcatccagc aggccaagct ggaggacctg accgacgaac tcgccaaggt cgccgagatc
     1681 acctggctgg acgacgttcg cgccaccatc ggtctggccg acaagctgtt cggcctcgcc
     1741 gcaggcatgg ccccacgccg gttccgcacc aagaccgacg ccgacggtcc gggcgtggtc
     1801 ctgttcacct cgggcagctt tggcgcgccc aagggcgtgg tgctgagcca gcgcaacctg
     1861 gtctcgaacg cacgccagat ctcggcgcac atcgcgatgg acgcggactg ggtgatgttc
     1921 aacccgctgc cgaccttcca ctcgttgggc ctgacgggcg gcgtcatcct gcctctgctg
     1981 cagggcatga aggcgtttga atatccgtcg ccgctgcatg ccaaacagat caccgacctg
     2041 ctgccaaagg tcggggcgtc gatcctgttc gcgaccgaca ccttcctgaa ccagtacggg
     2101 cgcgtcggtg aacccgagga cttcgcgacg ctgaagttcg tcgtcgccgg cgccgagaag
     2161 gtccgtgatg agacccggca cctgttcaac acccggttcg gcggggtgac gctgctcgag
     2221 ggctatggcg cgaccgaagc ggcgcccgtc attgcggtca accatcccga ccgcaaccgc
     2281 cccggctcgg tcgggcaggt cctgccgggc atcgaatacc gactggaaga cgttcccggc
     2341 atcaccggcg gcggtcagtt gtacgtgcgc ggtcccaacg tgatggaggg ctatatctcg
     2401 cccgatgatc ctctgggcct cgacatgctg cacgaggggt ggcacgacac gggcgatatc
     2461 gtcgacatcg acgccgacgg cta
//
LOCUS       DASVTM010000315        10735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_455180,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000315 DASVTM010000000
VERSION     DASVTM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455180"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VF633_08755"
     CDS             <1..221
                     /locus_tag="VF633_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX8471186.1"
                     /translation="KSLIHPSQIVGANAAFAPSPAEVAQAEAIVAAFADPASDGRGAI
                     RVNGAMVERLHLASAEWLLERYRVAVGG"
     gene            254..2317
                     /locus_tag="VF633_08760"
     CDS             254..2317
                     /locus_tag="VF633_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEX8471187.1"
                     /translation="MTIASPDPQSQTPAWVCAPGVLKVPFLTELLPEYDLCALPGSDV
                     EAVFGWGMKPTAAAGRRLAVKRGIPYVALEDGFLRSVGLGESGATSLSLIVDDLGVYY
                     DATRPSRLENLIRSAPDWCDAGMTARASGLIERIVASGVSKTNMGGPLDRSVLKPGRR
                     VLIVDQTLGDASIGYGLASTWSFGEMIAAARRDEPDAQLIVKRHPAVAAGRKQGCIAA
                     ADLTGVTLIDTDVRAAELLAQVDVVYVATSALGFEALLRGLPVRCFGAPFYSGWGLTT
                     DAVDTGRRGEQRTVEQVAAAALIQYTRYVDPITGRRCEAEQALNRLIRLRDRADRLQG
                     FWAAAGFAPAKRPPIRKLLNSPRGEVRYHWFARNAAKSAEDRGGKLIWWAGKESPPIL
                     EAAAATSAPTVRMEDGFIRSRGLGSDFFGALSVVLDDLGVYYDATGPSRLETLIETSD
                     YDTTALDRAARLRDRIVEAGLSKYNLKGEPPAGWPTDRERLLVVGQVENDRSIRLGCT
                     DIRTNSGLVEAARRDFPNAFLIYRNHPDVTAGNRPGLLDAGAMDAVDAVADDLDIIDC
                     LNACDRLVTLTSLSGFEALMRGKAVTTYGRPFYAGWGLTDDRSGPFERRSRRASLDEL
                     MHATLIDYPLYVTPTGWPCEAEDLVEALIAARDTPLPAPPRGRIVRWWRGIKASLDQT
                     PPPAY"
     gene            2388..3509
                     /locus_tag="VF633_08765"
     CDS             2388..3509
                     /locus_tag="VF633_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX8471188.1"
                     /translation="MIQSVIAATGLFTPEQSISNAELVGAYNAWAEQWNTDHAMEIAE
                     GDVAALTPSSEAFIEKASGIKSRYVLDKAGIIDPSRMAPNIPERGNDELSVMAEMAVA
                     AARQAIAAWGKPVSEIGAVLCAASNMPRPYPALAVEIQQALGIEGFGFDMNVACSSAT
                     FGIKTAADFIGSGSVKAVLMVNPEVCSAHLNFKDRDSHFIFGDVATAVIVEAADQAGP
                     GGWDILGTKLKTVFSNNIRNNFGFLNNAAPEGIGKPDKMFIQQGRKVFKDVVPLVSDM
                     IVDHAAELGVDPHGLKRLWLHQANINMNDYIGRKVLGRDPTPEENVIILDEYANTSSA
                     GSIIAFHLRHDGFEPGDTGLICSFGAGYSAGTVFVRKRV"
     gene            complement(3506..3961)
                     /locus_tag="VF633_08770"
     CDS             complement(3506..3961)
                     /locus_tag="VF633_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8471189.1"
                     /translation="MNLYSTVGFGFPFLNEIAPFVARIAVGGMFFLSGFYKLFTAASA
                     EKMQKTMVEAGIAAPRQTARFVSVCEFVFGALLILGLFTSVAAAVLLIISLVALITVA
                     AKSVEGASFGYRLSSYLDLPETLLMIILFWLICVGPGLWSLDWALLVPR"
     gene            complement(4019..5212)
                     /locus_tag="VF633_08775"
     CDS             complement(4019..5212)
                     /locus_tag="VF633_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8471190.1"
                     /translation="MTTFDPQAATAAWLATLSPADTAKAMAYTQGGHWLILWGFIVSI
                     VVAWIIIRTDVLGKVRTSIEKKKARPVLASFVVGLVYLVFSWVLNLPWAAYSSWYREG
                     QYGLTEQPFMGWFVEGMMSAAISTVVMGIVLIAVYALIRRARAFWWAWAAGLTAVVMV
                     IGLVVSPIVIEPLFNTYTPAPNGAVRDAVVDLAKATGTPSDKIFIYDGSKQSDRYTAN
                     VSGLFGSARVAMSDVMFTKGADLAEVRGVVGHEMGHYVHAHVLWILAVMIVLAALAFW
                     LTDRLYPVARKLLGANKVGDIRDPAGLPVLMAVLSFLSVLGTPLTNTLIRTIEADADH
                     FAMVHANEPDGLSKALIKTAEYRAPSPGKLEEFLFYDHPSVGNRIRAAMEWKAAHPAG
                     TPTKP"
     gene            5408..5689
                     /locus_tag="VF633_08780"
     CDS             5408..5689
                     /locus_tag="VF633_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471191.1"
                     /translation="MNDRPKKSAVPVEKLPFLESLARDVALVLKSLGGSAHQNVVIDC
                     VAAMKRNRGEPVGQDLRNAVVEAFERYTQWFTRPFGEGSMRWALVAEPA"
     gene            complement(5759..6286)
                     /locus_tag="VF633_08785"
     CDS             complement(5759..6286)
                     /locus_tag="VF633_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX8471192.1"
                     /translation="MRNILLATAAISLFAVPAMAQTLQSPTYYGTLGYSQFDHSDADL
                     GAVTGRLGAKVTPYLGAEVETSFGVRDDDFTVAGVEGSIEHRYDVAAYGVATLPLQPN
                     LELFARAGYGVTELKAEAAGLTSSDHYESFNYGAGANYYFDAQNGVRADWTRRDFQDD
                     GGEADVYSVNYVRRF"
     gene            complement(6471..7376)
                     /locus_tag="VF633_08790"
     CDS             complement(6471..7376)
                     /locus_tag="VF633_08790"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX8471193.1"
                     /translation="MTASKFSIAVVGPGAVGGTIAAWLSRSPLVSDLTLCARSPLDRL
                     VVETPDGTVIEARPRVVTDPGQITVPVDWVLIATKAYDVAGAALWLKPLLGPDTRVAV
                     LQNGVEHAERFAPFLSAARILPVIVDIPAERTAPGRIHHRRNGTVTVPAGEAGEAFVT
                     LFAESIIEASTANDFLTAAWTKLSLNCAGAVNALTGEPGGVVRAEGVADLMREMVWEC
                     VRVGRAVGADLGDHIPDWVVERAMTSPGDSVNSLLGDRLAGRPMEWDARNGVIVRLGQ
                     VHGVATPVNAMASTLLAAIGWPPSA"
     gene            complement(7612..10302)
                     /gene="ppdK"
                     /locus_tag="VF633_08795"
     CDS             complement(7612..10302)
                     /gene="ppdK"
                     /locus_tag="VF633_08795"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX8471194.1"
                     /translation="MTQWVYGFGGGSADGDASMKNLLGGKGANLAEMAKLGLPVPPGF
                     TVTTEACVQYFSNGNTYPGDLPGQVAAGLKTVESIVGKTFGDAANPLLVSVRSGARAS
                     MPGMMDTVLNLGLNDETVEGLANLSGDRRFAFDSYRRFITMYSNVVLGLSHDDFEEVL
                     DDHKDRLGVTVDTDLSASDWEKIVAEYKTVVERELGHPFPQDPQDQLWGAVSAVFASW
                     MNDRAKFYRRMHDIPESWGTAVNIQSMVFGNMGETSATGVAFTRNPSTGEARLYGEFL
                     INAQGEDVVAGIRTPQSLTKIGREEMGETAPSMEEAMPVVFAEFVDVVGRLESHYRDM
                     QDIEFTVEQGRLWMLQTRNGKRTAKSALKIAVDLAAEGVITQEEAISRVEPSALDQLL
                     HPTLDPNAVRKVVATGLPASPGAATGKIVFDADEAERMSQLGDAVILVREETSPEDIH
                     GMHAARGIVTARGGMTSHAAVVARGMGRPCVCGINALSIDEHAGTFTAHGRTFKAGEI
                     ITIDGSKGEVLEGAVAMIEPELTGDFQTLMAWADKVRRLKVRANAETPLDARTARGFG
                     AEGIGLCRTEHMFFDEERIAAVREMILADDEAGRRVALAKIAPFQKSDFVELFTIMAG
                     LPVTVRLLDPPLHEFIPHTDEDIDALAATSGIDAAKLKRRARELHETNPMLGHRGARL
                     GVAYPELYEMQVRAIIEAALEVKKTASEAPIPEIMHPLVAMGLEMKFLRELTDRTAKA
                     VFAEAGDTVEYLVGTMIELPRAALRAGDLAEYAEFFSFGTNDLTQTTFGISRDDSGRF
                     LQAYMDKGIFETDPFVRLDQEGVGDLIRIAKERGSAVRPDMKMGICGEHGGDPSSIAF
                     CEDVGLTYVSCSPYRVPIARLAAAQAALVSRGGTAREKDR"
     gene            10580..>10735
                     /locus_tag="VF633_08800"
     CDS             10580..>10735
                     /locus_tag="VF633_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8471195.1"
                     /translation="MPHADSLILTLVGGFVLAFVFGMLANRLKLSPLVGYLVAGIVVG
                     PHTAGFVA"
BASE COUNT         1890 a         3531 c         3456 g         1858 t
ORIGIN      
        1 gcaagagcct gatccatccg tcccagatcg tcggggccaa cgcggcgttc gcaccctcgc
       61 ccgccgaagt ggcccaagcc gaagccatcg tggcggcgtt tgccgaccct gcgtcagatg
      121 gacgaggcgc gatccgggtc aacggcgcaa tggtcgagcg cctgcacctc gcttcggccg
      181 aatggttgct ggagcggtat cgcgtggcgg tggggggcta ggcgcgacca gcgcccgccc
      241 ctagagacga cccgtgacta tcgcctctcc cgatcctcaa tcccaaacac ccgcctgggt
      301 ctgcgcgccc ggcgtgctca aggttccgtt tttgacggag ttactgcctg agtacgattt
      361 gtgcgccctg cccggctccg atgtcgaggc tgtgttcggc tggggcatga agccgacggc
      421 tgcggccggg cggcgactgg cggtcaaacg gggcatccct tacgtcgcgc tggaagacgg
      481 gttcctgcgt tcggtgggtc tgggtgagag cggcgcgacg tcgttgagcc tgatcgtcga
      541 cgatctcggc gtttattacg acgcgacccg tccctcgcgg ctggagaacc tgatccgttc
      601 cgcgccggac tggtgcgatg caggcatgac cgctcgtgcg agcgggctga tcgaacggat
      661 tgtcgcctcg ggcgtgtcga agaccaatat gggcgggccg ctcgaccggt cggtgctgaa
      721 gccgggccgc cgggtgctga tcgtggacca gactctgggc gacgcctcga tcgggtacgg
      781 cctcgcctcg acctggtcgt tcggcgaaat gatcgccgcc gcacgccgcg acgagcctga
      841 cgcccagttg atcgtcaaac gccaccccgc cgtcgccgca gggcgcaaac agggctgcat
      901 cgccgccgcc gacctgaccg gagtgaccct gatcgacacc gacgtccggg cagctgaact
      961 gctggctcag gtggacgtcg tctatgtcgc gacctcggct ctggggttcg aggccttgct
     1021 gcgcggcctg cccgtgcgct gttttggagc gcctttctat tccggctggg gcctgaccac
     1081 cgacgccgta gacaccggcc gtcgcgggga acagcgcacg gtcgagcagg tcgccgccgc
     1141 agccctgatt cagtacaccc gctatgtcga tccgatcacc gggcgccgct gcgaggccga
     1201 acaggcgctg aaccggctga tccgcctgcg cgatcgcgcc gaccgcctgc agggtttctg
     1261 ggccgccgcc gggttcgctc cggccaaacg tccaccgatc cggaagctgc tgaactcccc
     1321 acgtggcgag gtccgctatc actggttcgc ccgcaatgcc gccaagtccg ctgaggaccg
     1381 gggcggcaag ctgatctggt gggccggcaa ggagagcccg ccgatccttg aggccgccgc
     1441 cgcgacttct gctccgaccg tgcggatgga agacggattc atacgttcgc gaggtctggg
     1501 ctcggacttc ttcggggcgt tgtcggtcgt actggacgat ctcggcgtct actacgacgc
     1561 cacaggcccg agccgcctcg agacgctgat cgagaccagc gattacgaca ccaccgccct
     1621 cgaccgtgcc gctcgcttgc gtgaccgcat cgtcgaagcc ggtctgtcga aatacaatct
     1681 gaagggcgag ccgcctgccg gttggcctac cgatcgcgaa cgacttctcg tcgtcggtca
     1741 ggtcgagaac gaccgctcca tccggctcgg ctgcaccgat atccgcacca actccggtct
     1801 ggtcgaggcg gcccggcggg atttcccgaa cgccttcctg atctatcgca accatcccga
     1861 cgtcacggcg ggtaaccggc cgggtctgct ggacgcgggc gccatggacg ccgtggacgc
     1921 ggtcgccgac gatctcgaca tcatcgactg cctgaacgcc tgcgaccggc tggtgacctt
     1981 gacctcgctg tcggggttcg aggccctgat gcgcggcaag gctgtcacta cctacggccg
     2041 ccccttctat gccgggtggg gcctgaccga cgaccggtcc ggtccgttcg agcgccgcag
     2101 ccgtcgcgcc tcgctggacg agctgatgca cgcgacgctg atcgactatc cgctctacgt
     2161 cacgccgacc ggttggccat gcgaggctga ggatctggtc gaggcgctga tcgccgcccg
     2221 cgatacgccg ctgcccgccc ctccacgcgg ccggatcgtg cgttggtgga ggggtatcaa
     2281 agccagtctc gaccagacgc ctccaccggc ctattagacg gttcagaaaa agaacggacc
     2341 gcccctcgtg cgaggcgccg cccgccgagc gccagggtaa agtaccaatg atccagtccg
     2401 ttatcgccgc aaccggcctg ttcacccccg aacagtccat ctccaacgcc gagctggtcg
     2461 gggcctacaa cgcctgggcc gaacagtgga acaccgacca cgcgatggag attgccgagg
     2521 gcgacgtcgc cgccctgacg ccgtcctctg aagccttcat cgagaaggcc tcgggcatca
     2581 agtcccgcta cgtgctcgac aaggcgggga tcatcgatcc cagccggatg gcgccgaaca
     2641 tccccgaacg cggcaacgac gagctgtcgg tcatggccga gatggccgtc gccgccgccc
     2701 gacaggccat cgccgcttgg ggcaagcccg tcagcgagat cggcgccgtc ctttgcgccg
     2761 cctccaacat gccgcgtccc tacccggcgc tcgccgtcga aatccagcag gcgttgggca
     2821 tcgagggctt cggtttcgac atgaacgtcg cctgttcctc ggccaccttc ggcatcaaga
     2881 ccgccgccga cttcatcggc tcgggctcgg tcaaggccgt gctgatggtc aaccccgagg
     2941 tctgttcggc ccacctgaac ttcaaggatc gcgacagcca cttcatcttc ggcgacgtcg
     3001 ccactgccgt gattgtcgag gccgccgatc aggccggacc gggcggttgg gacatcctcg
     3061 gcaccaagct gaagaccgtg ttctccaaca acatccgcaa caatttcggc ttcctgaaca
     3121 acgccgcgcc cgagggcatc ggcaagcccg acaagatgtt catccagcag ggccgcaagg
     3181 tcttcaagga cgtggtgccg ctggtctcgg acatgatcgt cgaccatgcc gccgagttgg
     3241 gcgtcgatcc gcacgggctg aagcggctct ggctgcatca ggccaatatc aacatgaacg
     3301 actatatcgg gcgcaaggtg ctcggtcgcg acccgacgcc ggaagagaac gtcatcatcc
     3361 tcgacgagta cgccaacacc tcgtccgccg gatcgatcat cgccttccac ctgcgtcacg
     3421 acggcttcga gcctggcgac accggcctga tctgttcgtt cggcgcaggc tattcggccg
     3481 gtacggtgtt cgtgagaaag cgcgtctaac gcgggaccaa cagcgcccag tccaagctcc
     3541 aaagaccggg tccgacgcag atcagccaga acaggatgat catcagcaag gtctcgggca
     3601 ggtccagata gctggacagc cggtagccga agctggcccc ctcgaccgac ttggccgcga
     3661 cggtgatcag ggccacgagc gagatgatca gcagcaccgc cgccgcgacg ctggtgaaca
     3721 ggccgaggat cagcagcgca ccgaacacga actcacagac cgagacgaat ctcgccgtct
     3781 ggcgcggtgc ggcgatgcca gcctcgacca tggtcttctg cattttctcg gcgctggcgg
     3841 ctgtgaacag cttgtagaag ccggacagga agaacattcc cccgaccgcg atccgggcca
     3901 cgaagggcgc aatctcgttc aggaacggaa agccgaagcc cacggtggaa tacaggttca
     3961 tagggtcgtg ccgcctctgt tcccggctgc aatgcccggg aacagaagcg gttgctttct
     4021 aaggctttgt gggcgttcca gccggatgtg cggccttcca ctccatcgcc gcacggatgc
     4081 gattgccgac gctggggtgg tcgtagaaca ggaactcttc cagcttgccc ggcgacggcg
     4141 cgcgatattc ggcggtcttg atgagagcct tggataaccc gtccggttca ttggcatgca
     4201 ccatcgcaaa atggtcggcg tcggcctcga tggtgcggat cagggtgttg gtcaggggcg
     4261 tgcctaaaac gctcagaaaa gacagcaccg ccatcagcac cggcagcccc gccggatcac
     4321 gaatgtcgcc gaccttgttc gcacccaaca gctttctcgc gacgggatag agccggtccg
     4381 tcagccagaa ggccagagcc gccagcacga tcatgacagc gaggatccac agcacgtgag
     4441 cgtgcacata gtgacccatt tcatgaccca cgacgcccct cacctcggcg agatcggcgc
     4501 ccttggtgaa catgacgtcg ctcatcgcca cgcgggcaga accgaacagg cccgagacgt
     4561 tggcggtgta gcggtcggac tgcttcgagc cgtcgtagat aaagatcttg tccgacggcg
     4621 tgcccgtcgc cttggccaga tcaacgacgg catcgcggac agcaccgttc ggcgcgggcg
     4681 tataggtgtt gaacagcggc tcgatgacga tcggcgagac gaccagaccg atcaccatga
     4741 cgacagccgt cagccccgcc gcccacgccc accagaaggc tcgcgcacga cggatcaggg
     4801 cgtagaccgc gatcaggacg atacccatca cgacggtgga aatggccgca ctcatcatcc
     4861 cttcgacgaa ccaacccatg aacggttgct cggtgaggcc gtactgccct tcgcgatacc
     4921 agctgctgta ggccgcccaa ggcaggttca gcacccagct gaataccaga tacaccaggc
     4981 ccactacgaa ggacgccagc actggccgcg ccttcttctt ctcgatgctc gtccggactt
     5041 tgccgagcac gtcggtgcgg atgatgatcc acgccacgac gattgaaacg ataaagcccc
     5101 acaggatcag ccagtggccg ccttgggtat aggccatggc cttggccgta tcggcgggcg
     5161 atagcgtcgc cagccacgcg gcggtcgccg cctgcggatc gaatgtcgtc atggttttag
     5221 cccctcttcc gggcgggaga ggacgccaga caaggccggt tgtcagctaa attcgaggaa
     5281 aggtggcgag gagaaggcgc gccggttcac gaacaatcta ctgcaaagcc agcggcggcg
     5341 cttgcacgcg gtgggcgagt ttgcgaggaa ggctccatcg agaccgtgca cgtgagacga
     5401 catcccgatg aatgaccgcc cgaagaaatc cgctgtcccc gtcgagaagc ttccctttct
     5461 ggaatctctc gcgcgtgatg tcgcgcttgt gctgaaaagc ctcggtggct cggcccatca
     5521 gaatgtcgtc atcgactgcg tcgcggcgat gaagcgcaac cgcggcgaac ccgtcggtca
     5581 ggatctgcgt aacgcagtgg tcgaagcgtt cgagcgctat acccagtggt tcacccgacc
     5641 gtttggtgaa ggctcaatgc gctgggccct ggtcgcagaa cccgcctgaa tcaaacgtcc
     5701 atctgatgca aaaaggcgcg cccctttcgg agcgcgcctt ttgtctgtct gcgtcggcct
     5761 agaagcggcg gacgtagttg accgagtaga cgtcggcttc gccgccgtca tcctggaaat
     5821 cgcgacgggt ccagtcggcg cgaacgccgt tctgggcgtc gaagtagtag ttggcgccgg
     5881 cgccgtagtt gaagctctcg taatggtcgg agctggtcag cccagcggct tcggccttca
     5941 gttcggttac gccgtaaccg gcgcgggcga acagttcgag atttggctga agcggcaggg
     6001 tcgctacgcc ataggcggcg acatcatagc ggtgctcgat cgagccctca actccagcga
     6061 cggtgaagtc gtcatcgcga accccgaagg acgtttcgac ttctgcgccc agataaggcg
     6121 tgaccttggc gccgaggcga ccggttactg cgccgagatc agcatccgag tggtcgaact
     6181 gagaatagcc aagcgtcccg tagtatgtcg gcgattgtag cgtttgcgcc attgcaggaa
     6241 cagcaaacag ggagatagcg gcggtagcga gaagaatgtt acgcattgtt tcttatactc
     6301 ctagatgttg gtgacttggc gtcacccgcc ccatccagcg agttgttaaa tggtgtctca
     6361 ccttgaccgt tccatggcgg cactattaca aggtcgaaac tgtgggttcg ctgacacaga
     6421 ccgcgttcgc ggaaggctgt tacacgaccg tcgcggaagt ccctggatgt tcaggcggag
     6481 ggtggccagc cgattgcggc cagcagggtg gacgccatgg cgttgaccgg ggtcgcaacg
     6541 ccgtgaacct gtcccagccg gacgatgacg ccgttgcggg cgtcccattc catcggccgc
     6601 cccgccagcc ggtcgccgag cagcgagttg accgagtctc caggcgaggt catcgcccgc
     6661 tccactaccc aatccgggat gtggtcgccc agatcggcgc cgacggcccg gccgacgcga
     6721 acgcactccc acaccatctc ccgcatcagg tcggcgacac cctcggcccg aaccacgccg
     6781 cccggctccc cggtcagggc gttgaccgcg cccgcgcagt tcagcgaaag cttggtccag
     6841 gcggcggtca ggaagtcgtt ggcggtggaa gcctcgatga tgctttcggc gaacagggtg
     6901 acgaaggcct cgcccgcctc ccctgccgga acggtcaccg tcccattgcg acggtggtgg
     6961 atgcgacccg gcgccgtacg ctcggccggg atatcgacga tgacgggcag gatgcgggcg
     7021 gccgacagga agggcgcgaa ccgctcggcg tgctcgacgc cgttctgcag caccgcaacg
     7081 cgcgtgtccg gccccagcaa cggcttcagc caaagcgccg cccccgcaac gtcataggcc
     7141 ttggtcgcga tcaacaccca gtcgaccggc acggtgatct ggcccggatc ggtgacgacc
     7201 cgaggccggg cttcgatgac ggtgccgtcc ggtgtctcta cgaccagtcg gtccagcggc
     7261 gaccgggcgc acagcgtaag gtcgctgacc aacgggctgc gcgaaagcca tgccgcgatc
     7321 gtgccgccca cggcgcccgg accgacgacg gcaatggaga atttcgatgc agtcatggta
     7381 ggccctcggt aaggctcccc tctcccggcg ggagagggct tgagcgcacg atggtacagc
     7441 catcgttctt gcgcgaaagg gtgagggctg aacggctaga ggttggacgc ggcgattcca
     7501 gcactcgtga cgccaccggt tcaaattagc ccctcaccct ttcgccttcg gcgatcgctt
     7561 cgctcccgga ggctcaagct ctttcccggg gggagagggt gcaggcttgc cttaccggtc
     7621 cttctcgcgg gccgtcccac cgcgcgacac caacgccgcc tgggccgccg ccagacgggc
     7681 gatcggcacg cgatagggtg agcagctgac gtaagtcagc ccgacgtctt cgcagaaggc
     7741 gatcgacgac gggtcgccgc cgtgctcgcc gcagatgccc atcttcatgt ccggacggac
     7801 cgccgagccg cgctctttag cgatgcggat caggtcgccg acgccttcct gatcgagacg
     7861 cacgaacggg tcggtctcga agattccctt gtccatatag gcctgcagga accggccgga
     7921 gtcgtctctg gagatgccga acgtcgtctg ggtcaggtcg ttggtgccga acgagaagaa
     7981 ctcggcatac tcggccagat caccggcgcg tagggctgcg cgtggcagct cgatcatcgt
     8041 gccgacgaga tattccaccg tgtcgcccgc ttcggcgaag acggccttgg cggtccggtc
     8101 cgtcagttcg cgcaggaact tcatttccag acccatggcc accagcgggt gcatgatctc
     8161 ggggatcgga gcctcggagg cggttttctt gacctccagc gcggcctcga tgatcgcccg
     8221 aacctgcatc tcgtagagtt cgggataggc gacgccgagg cgggcgcccc ggtgacccag
     8281 catcggattg gtctcatgca gctcacgcgc ccggcgcttc agcttggccg cgtcgatgcc
     8341 cgaggtcgcc gcgagtgcgt cgatgtcctc gtccgtgtgc gggatgaact cgtgcagcgg
     8401 cgggtcgagc aggcgcaccg tcaccggcag gccggccatg atggtgaaca gctcgacgaa
     8461 gtccgacttc tggaacggcg cgatcttggc cagcgccacg cggcgaccgg cctcgtcgtc
     8521 ggccaggatc atctcgcgca cggcggcgat ccgctcctcg tcgaagaaca tatgctcggt
     8581 gcggcacagg ccgatgccct cggcgccgaa accgcgcgct gtgcgggcat ccagcggcgt
     8641 ctcggcgttg gcgcgaacct tgagacggcg aaccttgtcg gcccaggcca tgagggtctg
     8701 gaagtcgccg gtcagctcgg gctcgatcat ggcgacggcg ccctccagca cttcgccctt
     8761 ggagccgtcg atggtgatga tctcacccgc cttgaaggtg cggccgtgag cggtgaaggt
     8821 gcctgcgtgc tcgtcgatcg acagggcgtt gatgccgcag acgcagggac gccccatgcc
     8881 acgagccacg acggcggcgt ggctggtcat cccgcctcgc gccgtcacga tgccccgggc
     8941 ggcgtgcatg ccgtgaatgt cctcgggcga ggtctcttcg cgcaccagaa tgacagcgtc
     9001 gccgagttgc gacatccgct ctgcctcatc ggcatcgaac acgatcttgc cggtcgccgc
     9061 gccggggctc gcgggcaggc ccgtcgccac gaccttgcgc acagcgttcg gatcaagcgt
     9121 cggatggagc aactggtcaa gggcggaagg ctcgacccgg ctgatcgcct cttcctgcgt
     9181 gatcacgccc tcggcggcga gatcgacggc gatcttcagc gcggacttgg ccgtgcgctt
     9241 gccgttgcgg gtctgcagca tccagagacg gccctgttcg accgtgaatt cgatgtcctg
     9301 catgtcgcgg tagtggcttt ccagccgccc gacgacgtcg acgaactcgg caaacaccac
     9361 cggcatcgct tcttccatcg agggagcggt ctcgcccatc tcctcgcggc cgatcttcgt
     9421 tagcgactgg ggcgtgcgga tgccggcgac gacgtcctcg ccctgtgcgt tgatcaggaa
     9481 ctcgccgtac aggcgcgcct cgccggtcga ggggttgcgg gtgaaggcga cgccggtggc
     9541 cgaggtctcg cccatgttgc cgaagaccat cgactggatg ttgacggcgg tgccccagct
     9601 ttcggggatg tcgtgcatgc ggcgatagaa tttggcccgg tcgttcatcc agctggcgaa
     9661 cacggccgag acggcgcccc acagctggtc ctgcggatcc tgcgggaacg gatggcccag
     9721 ctcacgctcg accacggtct tgtattcggc gacgatcttt tcccagtcgc tggcggacag
     9781 gtcggtgtcg acggtgacgc cgagacgatc cttgtggtcg tccagaacct cctcgaaatc
     9841 atcgtgcgac aggccgagca ccacgttgga atacatggtg atgaagcggc gatagctgtc
     9901 aaaggcgaag cggcgatcgc ccgacaaatt ggctagaccc tcgaccgttt catcgttgag
     9961 gccgaggttc aggaccgtgt ccatcatacc cggcatcgag gcgcgggcgc ccgaacggac
    10021 cgacaccagc agagggtttg cggcgtctcc aaacgtcttg ccgacgatcg attccacggt
    10081 cttgagaccg gccgcgacct gcccgggcag atcaccggga taggtgtttc cgttcgagaa
    10141 atactggacg caggcctcgg tggtgacggt gaaacccgga ggcacgggca gacccagctt
    10201 cgccatctcg gccaggttcg cgcccttgcc gccgagaagg ttcttcatcg acgcgtcgcc
    10261 gtcagcggac ccgccgccaa agccgtacac ccactgagtc atctgtagcc cctgagtctg
    10321 gtaacctgag tttggcgata agatgggcag gttgacgtcc cgctcctgac ggtctgacta
    10381 ccgtgctgcg actgcgaagg cgagcccggg ctgtaacaaa gcgtgagaat tctctagaat
    10441 cgcgagcctg ggggcgcgga ccactgaggg aatccgagct gggccgggac atatcgtcgc
    10501 gttgtcccga agcctcacgg ttcccacttg tggacctcgc cacaactgaa accaagagcc
    10561 caccacgcaa ggagcgccga tgccgcacgc cgacagcctg attctcaccc tggtcggggg
    10621 tttcgtcctc gccttcgtgt tcggcatgtt ggcgaaccgg ctcaaactgt ctccgttggt
    10681 cggctatctg gtcgccggga tcgttgttgg accacatacg gcgggtttcg tcgcg
//
LOCUS       DASVTM010000316        10932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_134912,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000316 DASVTM010000000
VERSION     DASVTM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134912"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..614
                     /locus_tag="VF633_08805"
     CDS             141..614
                     /locus_tag="VF633_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX8471196.1"
                     /translation="MTFTLTSTDVVDGRVLNDAQVQAKGNTSPQLSWSGAPQGTKSYA
                     ITVYDPDAPTGSGFWHWTVANIPADVTELATGASSNGLPKGAVEGRTDYGAPGFGGAA
                     PPPGHGPHRYIFTVFAVDVDRLPVTAEDSGAKYGFNLHFHTLAKASITATYENRG"
     gene            complement(618..1769)
                     /locus_tag="VF633_08810"
     CDS             complement(618..1769)
                     /locus_tag="VF633_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX8471197.1"
                     /translation="MSSTAAYADTYGKVDDRTSDEPVWQWASAYTADVIGVVDGGETR
                     AGRFLDNLDVSLDGDLERQFGWPSARVHFSILANGGGEPNAIAQTLQGYDNIEVSSQG
                     VRLYEAWLEQDLADGAVSVLAGLYDVNSEFYSTEASGLLIAPPFGIGSELAATGPNGP
                     AIFPSTSLAVRVRVGAADGLHARFAAVNARAGTIGDDGGVDTTLDDGILYLFEAGWTG
                     PVRIAVGGWRYGENQEDIRDVSASGDPVLTDAQGAYVVAEGTIFEAEQAPTGRAFVRL
                     GISDGDTTDFNGGWQAGMRVDSIFRSRPDSALSVGFHQGLLSDKARANALDAGTTFAN
                     AEGGVELTYADTFGRFTIQPDLQWIYNPGGDKNRDPILTAGLRVILKLR"
     gene            1993..3801
                     /locus_tag="VF633_08815"
     CDS             1993..3801
                     /locus_tag="VF633_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8471198.1"
                     /translation="MLTTIRSKVKFAGLFMVILCALGSGSGLLIAGFLSGSMGDSTRI
                     GEVMRNHMQADMMHDALRSDVLAAMLAAQNGQADAMAGIRTELREHSDSFRMAVEENN
                     AMARDPGTITALKEAATPLEQYIASADRIVTLAAADPGAANAALPAFKTSFVALEGRM
                     GAISEQIESTQLATKARADFLAKGGIVVMILIILLSIGLSLALIRLSMRTVVAPIGVL
                     DDEMAAFAQGRTDVELTSSNRKDELGAVGRSIVALQALIVERARAEAVAQAAQRTKGE
                     QAQRAIAEQQALIVAAMTEGMDHLMRGDLTYRIASDFPDGYEKLKDDFNAAVIGLEET
                     LVSVDGNASTIRSGAHEMTHAADDLSRRTEQQAASLEETAAALEQITATVNRSAEGAR
                     KASDVVGEARDEAETSGRVVGQAVEAMDAIHASSNEMGQIISVIDEIAFQTNLLALNA
                     GVEAARAGDAGRGFAVVASEVRALAQRSASAAKEINALISNSGRQVSLGVDLVRQAGS
                     NLDKIANRVVHVGSLITEMAAASQEQATALREINTAVSHMDQVTQQNAAMVEQTTAAS
                     HALAQETEVLRQSVGRFRLSGRSQSVTEDLPMRVAA"
     gene            3853..4515
                     /gene="purQ"
                     /locus_tag="VF633_08820"
     CDS             3853..4515
                     /gene="purQ"
                     /locus_tag="VF633_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX8471199.1"
                     /translation="MSAAVIVFPGSNCDRDCKVAVERSTGERVEMVWHQDTELPDDLD
                     LIVLPGGFSYGDYLRCGAMASQAPVMQAVKKAADDGVAVVGICNGFQILCEAGMLPGA
                     LLRNAGLKYVCKPISLEVTNGQTRFTAGYQGQREVVMTQGNGDGNYFADAETLARLEG
                     EGQVVFRYVDNPNGSVANIAAIQNARGNVLGMMPHPDRAFEAELGSADGATLFRSVMA
                     AA"
     gene            complement(4545..4793)
                     /locus_tag="VF633_08825"
     CDS             complement(4545..4793)
                     /locus_tag="VF633_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471200.1"
                     /translation="MADPEDAQQETPHFETDDVEANLAIEQGLGVGARELAAQRDPGG
                     VVTPDEDDGDDADAAIERNTSVLGQDDDDQQLSRNSDA"
     gene            4908..5699
                     /locus_tag="VF633_08830"
     CDS             4908..5699
                     /locus_tag="VF633_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471201.1"
                     /translation="MSIVRPTASQQTRRWTVLGAAIFAIAIAQVQMGLGWGQSQAEFA
                     ADSDATLKVIGWAFAIWGVIYLGLAIYAIRQVLPQTGESDLIHAFGWPSVLAFAGIGL
                     WIVAAAFDWEVATIVLIFGALIVLLIPLLTNARRIRALSMGDRDRWMTVWPLSLLAGW
                     LTIAAPVNLLTVATGNGDLPTALSPTAWAILAVVAVSVVAVFVTWRIRTIVYGLPVSW
                     GLIGVFAAEQSRNSALAFTAVIAAVAVLVIDVVIVLAQRRRVERV"
     gene            complement(5696..5851)
                     /locus_tag="VF633_08835"
     CDS             complement(5696..5851)
                     /locus_tag="VF633_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471202.1"
                     /translation="MTDQPIDPAATPEDDDAPEIAADDVGASAIAERFGKQAVDDEEE
                     DVDGDGI"
     gene            5953..8151
                     /gene="purL"
                     /locus_tag="VF633_08840"
     CDS             5953..8151
                     /gene="purL"
                     /locus_tag="VF633_08840"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX8471203.1"
                     /translation="MNAPEKTISELAAEYGLSPAEYQVVLDRLGREPNQLELGVFSVM
                     WSEHCSYKSSKIHLGKFPTTGPRVICGPGENAGVIDIGDGDACIFKMESHNHPSYIEP
                     YQGAATGVGGIMRDVFTMGARPVALLNALRFGDIGHEKTKRLVKGVVSGIGGYGNCVG
                     VPTVAGETNFHSGYNGNILVNAMCVGVAKADSIFYSAAPGPNMSVVYFGSKTGRDGIH
                     GATMASAEFDEDSDEKRPTVQVGDPFAEKLLIEATLELMASGAVAAIQDMGAAGLTSS
                     SVEMAGKGGVGIELNMDAVPQRETGMSAYEMMLSESQERMLAVLKPGREEDGYRIFKK
                     WGLDAAVIGMTTETGRLILNHHGEVVCDVPLAPLFDDAPLYDRPWVQPELHARLTAAD
                     VPAPESWEDAVLKVLTCPDMASKRWIWEQYDRHVMADTLQDSATGADAGVVRVHGTDK
                     GLAVTSDCTPRYVQNDPYEGGKQCVAEAWRNLVSVGALPLAITDNLNFGHPQRPEIMG
                     QIVRAIDGMAEACRELDFPVVSGNVSLYNETNGVAIPPTPTVGGVGLLENYDVVTGFS
                     TMAEGDTLVLIGQTHGELGASIYLREVLGREDGAPPPVDLKLERKTGDFMREMIEGGR
                     LTCVHDLSDGGLVGAAADMALASDTGVVLDASSATHAHILLFGEDQARYLVAVPNPQE
                     VLAEAREAGLHASVVGHARGDAFASTDLFSIPLAHLREIHEGWMPGWIEG"
     gene            8156..8644
                     /locus_tag="VF633_08845"
     CDS             8156..8644
                     /locus_tag="VF633_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471204.1"
                     /translation="MRLPILAIAMIALAACDGEDPVTSATREISTANHAAAAVAEAEA
                     KAEASDQAKPPASATVPVLATECDVEITFGSACCGVDGDLRAKLLAVTSADPDVAESS
                     EKRWGREGESTVCLKMSDPAAADRLYEAIATQLPSTSSKAPTTVRNRDGLSRATAYSV
                     GR"
     gene            8685..9779
                     /locus_tag="VF633_08850"
     CDS             8685..9779
                     /locus_tag="VF633_08850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8471205.1"
                     /translation="MRVVIVLPPGSRFDALRPTSIETVVRSMGLHSRDRNTLTIICDE
                     GGERGGDLNTLAIAPERSPEKRARIVAAHIQRLQPDLVEVHQHMPSAAALAKRLRDIP
                     MVLYRHNLLPPTGNPLKAWRHRRRAALFHGHVFVSEIGRKQFADQYPHLAERAVSITN
                     AIDPALWKAPVAGRAPIIAFAGRAAPEKGLAPLCLALATALDAAPDWRAELALSDWST
                     HQAWSEAQVAPLARFGGRVKIVHNAPHDAVRGLFQRAAIVAIPSLWQEPFGLVAIEAH
                     AAGAAVLSSGLGGLAEASGGHALVVTPEADFAERLAEGLLWLAEHPVEREALAQAGQV
                     YITTAHDAHDRSQQLDATRRNFMEVSRIRL"
     gene            9933..>10932
                     /locus_tag="VF633_08855"
     CDS             9933..>10932
                     /locus_tag="VF633_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8471206.1"
                     /translation="MWSERHFKEPQLFGSGRFGEIIVVSHPAGVRHVLTENAANYEKG
                     ALQRRVLGPMLADGLLLTEGDVWRRARRILAPLFTPARTLKMAARMDAVCRARVEALH
                     LDHGARVLNIDSEMSGLTFDILSATLFSDELAGDARGFERALNQYLSVGGRIDPLDVI
                     NAPGWIPRLGRTASYRSAKFFEQRVGALVARRRARIEAGDDTPDDLLTALLTARDEGE
                     DGKGLDDREVAANILTFILAGHETTARTLGWTLHLISRSPETATRLKTEADAFDVTDP
                     KWAEGLPWMRAVIEEAMRLFPPAPTLARRALGPDIIGGQAISTGAAVVISPWLLHRH"
BASE COUNT         1947 a         3551 c         3504 g         1930 t
ORIGIN      
        1 ctgaaaggtt ctggccagga ggacctggag catgaagcgc tcgcgttcct gaggacaagg
       61 aactcagttg gtgacagact gtcgcaacgc aacgcccgcc ccgtcaacgc gttggccaaa
      121 gtcgtcagac agagacacca atgaccttca ctctcacctc taccgatgtc gtcgatggcc
      181 gcgttctgaa cgacgcccag gtccaggcca aggggaatac ctcgccgcag ctgagctggt
      241 ccggcgcgcc ccaaggcaca aagtcctacg ccatcaccgt ctatgatccc gacgcgccga
      301 ccggatcggg cttctggcac tggactgtcg ccaacatccc cgcagatgtc acagaactgg
      361 cgaccggtgc gtcatcaaac ggcctgccta agggcgcagt cgagggacgc acggattacg
      421 gagccccggg ctttggcggg gccgcaccgc cgcccggcca tggtccgcac cgctacatct
      481 tcaccgtgtt cgccgtcgac gtggaccgtc tgccggttac ggccgaggat tcgggcgcca
      541 aatacggctt caacctgcat ttccacaccc tcgcgaaagc ctcgattacc gcgacctacg
      601 agaaccgcgg ctaagcgcta cctgagcttc aggatcacgc gcagaccggc cgtaagaatg
      661 ggatcgcggt tcttgtcgcc accggggttg tagatccatt gaaggtcggg ctggatcgtg
      721 aaacggccga atgtgtcggc gtatgtcagc tccaccccgc cctcggcatt ggcgaatgtg
      781 gtcccggcgt ccaatgcatt ggccctggcc ttgtccgaca gcagaccctg gtggaagcct
      841 accgataatg cgctgtccgg gcgggagcga aatatcgaat ccacgcgcat gccggcttgc
      901 catccgccgt tgaagtccgt ggtgtcgccg tctgatatgc ccagccgcac gaacgcgcgc
      961 cccgtcggcg cctgctcggc ctcgaagatg gttccttcgg ccacgacgta ggcgccttgg
     1021 gcatcggtga ggacagggtc gcctgaggcg gagacgtcgc gaatgtcttc ctggttctcg
     1081 ccataccgcc agccaccgac cgcgatccgc acgggaccgg tccagccagc ctcgaagaga
     1141 tagagaatgc cgtcgtcgag ggtggtatcc acgccgccgt cgtctccgat cgtccctgcg
     1201 cgagcgttga ccgccgcaaa ccgggcgtga agcccgtccg ccgccccgac ccggacgcgc
     1261 acggccagag acgtcgaggg gaagatcgca ggtccgttcg gaccggtggc cgccagttcg
     1321 gaaccgatgc cgaaaggagg ggctatcaag aggcccgagg cctcggtgct gtagaactcg
     1381 ctgttgacgt catagaggcc cgccagcacc gagaccgcgc catcggcgag atcctgttcc
     1441 agccaggcct cgtaaaggcg caccccctgg gatgagactt cgatattgtc gtagccttgt
     1501 agcgtctggg cgatggcgtt gggctcaccc ccgccattgg ccagaatgct gaaatgaact
     1561 cgcgcggacg gccagccgaa ctggcgttcc aggtcgccgt cgagcgagac gtcgagattg
     1621 tccaggaaac gccctgcgcg ggtctcgccg ccatcaacga cgccgatgac gtcggcggta
     1681 taggctgagg cccactgcca gactggttcg tcagaggtcc ggtcgtcgac cttgccgtaa
     1741 gtatcggcgt aagcagccgt agatgacaaa agtacaccta aagcaataaa tccacatact
     1801 tgatgcagag acatcatagg ataaaccatc cattctggaa ggatgggcat cgtcgacacg
     1861 gacccacaat gttcgtgatg atcgtccgaa gatctcaaga aaacgccagt ttcccaatag
     1921 gtagtgctac ttatcgcaga gaacggcgat cgttaggatt tgacacgtag gcaagggtga
     1981 acgggggacc tcatgctcac aacgatccga tccaaggtga aattcgcggg gctgttcatg
     2041 gtgatcctgt gcgcgctggg ctctggctca gggctcctga tcgcgggctt cctcagcggc
     2101 tcgatgggag attcgacgcg gatcggcgag gtcatgcgca accacatgca agctgacatg
     2161 atgcatgacg ccctgcggag cgacgtgctc gccgccatgc tggcggccca gaacggccaa
     2221 gccgacgcca tggcgggaat tcgcaccgaa ttgcgcgaac actccgacag cttccgcatg
     2281 gccgtcgaag aaaacaacgc gatggcccgt gatcccggca caatcactgc attgaaagag
     2341 gccgccaccc cgctcgaaca atatatcgcc tcagccgatc ggatcgtgac gttggcggca
     2401 gccgatcctg gtgcggccaa cgccgcactt cccgcattca agaccagttt cgttgcgctg
     2461 gaaggtcgaa tgggggccat ctcggagcag atcgagtcca cccagctggc caccaaggct
     2521 cgcgccgact tcttggccaa gggcggcatc gtcgtcatga tccttatcat tctgctcagt
     2581 atcggactga gcctggctct gatccggctg tcgatgcgaa cggtcgtggc ccccatcggc
     2641 gttctcgacg acgaaatggc cgctttcgcg caggggcgaa cggacgtgga actgaccagc
     2701 tccaatcgta aggatgagct cggcgccgtc ggccggtcga tcgtagccct tcaggccctg
     2761 atcgtcgaac gggcccgtgc ggaagccgtc gctcaggcgg cgcagcgtac caagggcgag
     2821 caggcccaga gagccatcgc cgaacagcag gcgctgatcg tagccgcgat gaccgagggt
     2881 atggaccatt tgatgcgagg cgacctgacc tatcggatcg ccagcgattt tcccgacggc
     2941 tatgaaaagc tgaaggacga tttcaacgca gccgtaatcg gcctggaaga gacgctggtt
     3001 tccgtcgacg ggaacgcttc gacgatccgc agcggcgccc acgagatgac ccacgcggca
     3061 gacgatctct cgcgccgtac cgagcagcag gccgccagcc tggaagagac ggccgccgcc
     3121 ctcgaacaga tcaccgcgac agtgaatcga tcagccgaag gcgctcgcaa ggcctccgac
     3181 gttgtcggcg aggcccgcga cgaggccgag accagcggaa gggtggtcgg tcaggccgta
     3241 gaggccatgg acgcgattca cgcctcatcc aacgaaatgg gccagatcat cagcgtcatc
     3301 gacgaaatcg ccttccagac gaaccttctg gccttgaacg cgggagtgga ggccgcgcgt
     3361 gcgggcgacg cagggcgcgg cttcgcggtc gtcgcctccg aagtgcgggc gctggcccag
     3421 cgctccgcga gcgcggccaa ggagatcaat gccctgatct cgaattccgg tcgccaggta
     3481 tcactggggg tcgatctggt gcgccaggcg ggttcgaacc tcgacaagat cgccaaccgg
     3541 gtcgtccacg tcggatcgct catcaccgag atggccgccg ccagtcagga acaggcgacg
     3601 gccctgcgcg aaatcaatac ggccgtgagc cacatggacc aggtcaccca acaaaacgcc
     3661 gccatggtcg aacagactac tgcggccagc catgccctcg cacaggagac cgaagtgctg
     3721 cgccagtcgg taggtcgttt ccgcctgtcg ggacggtctc agtcggtgac tgaagatctt
     3781 ccgatgcgtg tggccgcctg atcgcctcca tcagccctga cagcctgatg gaatcctgct
     3841 aaggccccgc ccatgagcgc agctgtcatc gtcttccccg gatccaactg tgaccgcgac
     3901 tgcaaggtcg ctgtcgaacg ctccaccggt gagcgggtcg aaatggtctg gcatcaggat
     3961 actgagcttc cggacgacct cgacctgatc gtcctgccgg gcggcttttc gtatggtgat
     4021 tacctgcgtt gcggcgcgat ggcgtcgcag gcgccggtaa tgcaagccgt caagaaggcc
     4081 gctgatgacg gcgttgccgt ggtcggcatc tgcaacggct tccagatcct gtgcgaagcc
     4141 gggatgctgc cgggcgcact gctgcgcaac gccggcttga aatacgtctg caagcccatc
     4201 tctctggaag tcactaacgg gcagacccgc ttcaccgccg gttatcaggg tcagcgcgaa
     4261 gtggtcatga cccagggcaa cggcgacgga aactatttcg ccgacgccga gacgctggcc
     4321 cgcctcgaag gcgagggcca ggtggtgttc cgctacgtcg acaatccgaa tggttcggtg
     4381 gctaacatcg cggccatcca gaacgcacgc ggcaacgtcc tcggcatgat gccccaccct
     4441 gaccgggcct tcgaagccga actcggttcg gctgacggcg caacgctgtt ccgcagcgtg
     4501 atggcagccg cctgaccatg tgagacgatc aggcggcgct tgtctcaggc gtcgctgttc
     4561 ctggaaagtt gctggtcatc gtcgtcctga cccaggacgg acgtgttgcg ttcgatagct
     4621 gcgtctgcgt catcaccatc gtcctcgtcc ggcgttacaa cacctccggg atcgcgctgg
     4681 gccgcaagct cgcgggcgcc gacgcccaga ccctgttcga tggccagatt ggcctcgacg
     4741 tcgtcggtct cgaagtgtgg cgtctcttgc tgggcgtctt ccggatcagc catttcggcc
     4801 tcctgatatg attgaggatg cgggctgaac caaccgcgac ggtggcggtt ccggtgaacc
     4861 atcaaggctc ctgtgcgtat gcggcctgaa cctccgcgag aacatgcatg tccatcgtcc
     4921 gcccgacagc ctctcaacag acccgccgat ggaccgttct gggcgctgcg atcttcgcca
     4981 ttgcgattgc gcaggttcag atgggccttg gctggggcca gagtcaggcc gagttcgcgg
     5041 ccgacagcga cgccacgctg aaggtcatcg gctgggcctt cgccatctgg ggtgtcatct
     5101 atctgggact ggcgatctat gcgatccggc aggtcctgcc gcagaccggc gagagcgacc
     5161 tgatccacgc cttcggctgg ccgtctgtgc tggcgtttgc cgggatcggc ctctggatcg
     5221 tggcggcggc gttcgattgg gaagtcgcaa cgatcgtgct gatcttcggg gcgctgatcg
     5281 ttctactgat cccgttgctc accaacgccc ggcgcatccg ggcgctgtct atgggtgaca
     5341 gggatcgctg gatgaccgtt tggccgctga gcctgctggc gggctggctg accatcgccg
     5401 cgccggtgaa cctgcttact gtggcgaccg gtaatggcga cctgcctacc gctctgtcgc
     5461 caacggcttg ggccatcctc gccgtggtgg cggtatcagt cgtggcggta ttcgtgacct
     5521 ggcgcatccg gaccatcgtc tacggtcttc cggtttcctg gggactcatc ggtgttttcg
     5581 cggccgagca atcgaggaac agtgcgctgg cgttcaccgc cgtgatcgcc gccgtcgctg
     5641 tgctggtcat cgatgtagtg atcgtcctgg ctcagcgacg gcgcgtcgaa cgggtctaga
     5701 taccgtcgcc gtcgacgtct tcttcttcgt catccaccgc ctgcttgccg aagcgttccg
     5761 caatagctga cgcgccgacg tcatcggctg caatttcggg ggcgtcgtcg tcttcgggcg
     5821 tggctgcggg gtcgatgggc tggtcggtca tggcgatctc ctgaggggct ccgcgattca
     5881 atcgccacag agcgtcgatg ttcccttccc tgcgggctga ctccggcctc gaacatcgct
     5941 agaagccccg ccatgaacgc ccccgaaaag accatctccg aattggccgc cgaatacggc
     6001 ctgagccccg ccgagtatca ggtcgtcctc gaccggctgg gccgcgagcc caaccagctc
     6061 gagctcggcg tgttctcggt gatgtggtcc gagcactgct cgtacaagtc gtccaagatc
     6121 catctgggga aattcccgac caccggcccg cgcgtcatct gcggtccagg cgagaacgcc
     6181 ggggtcatcg acatcggtga cggggacgcc tgcatcttca agatggagag ccacaaccac
     6241 ccctcctaca tcgagcccta tcaaggcgcg gcgaccggcg tcggcggcat catgcgcgat
     6301 gtcttcacca tgggcgcgcg tcctgtggcg ctgctgaacg cgctccgttt cggcgacatc
     6361 ggccacgaga agaccaagcg tctggtcaag ggcgtggtgt ccggcatcgg cggctatggc
     6421 aactgcgtcg gcgtgccgac cgtggccggc gagaccaatt tccactccgg ctacaacggc
     6481 aacatcctcg tcaacgccat gtgcgtcggg gtcgccaagg cggacagcat cttctattcg
     6541 gccgcgccgg gaccgaacat gtcggtcgtc tattttggct ccaagaccgg ccgcgacggc
     6601 atccacggcg cgaccatggc ctccgcagag ttcgacgagg actccgacga gaagcgcccc
     6661 acggttcagg tcggcgatcc cttcgccgag aagctgctga tcgaagcgac tctggagctg
     6721 atggcctcgg gcgcggtggc tgcaattcag gatatgggcg cggcgggcct gacctcgtcg
     6781 tcggtcgaga tggccggcaa gggcggcgtc ggcatcgagc tgaacatgga cgccgtgcct
     6841 cagcgcgaga ccggcatgtc cgcctatgaa atgatgctct ccgaaagcca ggagcggatg
     6901 ctggccgtgc tgaagccggg ccgcgaggaa gacggctatc gcatcttcaa gaagtggggc
     6961 ctcgacgccg ccgtcatcgg catgacgacc gagaccggtc gcctgatcct gaaccaccat
     7021 ggcgaggtcg tctgcgacgt gccactggcg ccgctgttcg acgacgcgcc cctgtatgat
     7081 cgcccttggg ttcagccgga gctgcacgcc cgcctgaccg ccgccgatgt ccccgctccg
     7141 gagagctggg aagacgcggt tctgaaggtg ctgacctgtc ccgacatggc gtccaaacgc
     7201 tggatctggg agcagtacga ccgccacgtg atggccgaca ccctgcagga ctcggcgacg
     7261 ggtgcagacg ccggcgtcgt gcgcgttcac ggcaccgaca agggtcttgc cgtgacctcg
     7321 gactgcacgc ctcgctatgt ccagaacgac ccctatgaag gcggcaagca gtgcgtcgcc
     7381 gaggcctggc gcaatctcgt cagcgtgggc gccctgcccc tcgcgatcac cgacaacctg
     7441 aacttcggcc acccgcagcg tcccgagatc atgggccaga tagtgcgggc catcgacggc
     7501 atggccgagg cctgccgcga gttggacttc cccgtcgtga gcggaaacgt cagcctctat
     7561 aacgagacca acggcgtcgc cattccgccc accccgaccg tgggcggcgt gggtctgctt
     7621 gagaactacg acgtggtcac aggcttctcc accatggccg agggggacac tctggtgctg
     7681 atcggccaga cgcacggcga actgggcgca tcgatctatc tgcgtgaggt tctgggccgt
     7741 gaagacggcg ccccgccgcc ggtggacctg aagctggagc gcaagacggg cgacttcatg
     7801 cgcgagatga tcgaaggagg ccggctgacc tgcgtccatg atctgtccga tggagggctg
     7861 gttggcgctg cggcggacat ggcgctggcg tctgacacag gtgtcgtgct cgacgcgtcc
     7921 agcgcgaccc acgcccacat cctcctgttc ggagaggatc aggcccgcta tctggttgcc
     7981 gttccgaacc cgcaggaagt tctggccgag gcgcgtgagg cggggctgca cgcgtcggtc
     8041 gtcggccacg cccgaggcga cgcgtttgcc tccacagacc tgttctccat cccgctggcg
     8101 cacctgcgcg agatccacga aggctggatg cccggctgga ttgagggtta gtcctgtgcg
     8161 gctgccgatc ctcgccattg ctatgatcgc tctcgccgct tgcgacggcg aagacccagt
     8221 caccagcgcc acgcgggaaa tctcgacggc caaccacgcg gccgccgcag tcgcagaggc
     8281 cgaagccaag gctgaggcgt cggatcaggc caaacctccg gcttccgcga cggttccggt
     8341 tttggcgacc gaatgcgatg tcgagatcac gttcggcagc gcctgttgcg gcgtggatgg
     8401 cgatctccgt gcgaagcttc tggccgtgac aagcgccgac cccgacgtgg ccgagtcgtc
     8461 cgagaaacgc tggggtcgcg agggggaatc gaccgtctgc ctcaagatga gcgacccggc
     8521 cgccgcagac cggttgtatg aggcgatcgc aacccaactt ccctcgacca gctcgaaggc
     8581 gccgacgacc gtcaggaacc gggacggact cagtcgcgcg acagcgtatt ccgttggccg
     8641 ctgatcagcc gccacccgct cctgccatcc ctttcggacg ccaaatgcga gtcgtgatcg
     8701 tcctgcctcc ggggtcgcgg ttcgacgccc tgagaccgac gtcgatcgag acggtcgtgc
     8761 ggtcgatggg gctgcacagt cgcgaccgga acacgctgac gatcatctgc gatgaaggtg
     8821 gcgagcgcgg cggagacctc aacaccctgg ctatcgcccc ggagcgatcg ccagagaaac
     8881 gcgcccggat cgtcgcggct catatccagc ggctgcagcc cgatctcgtc gaggttcatc
     8941 agcacatgcc tagcgcggcg gctctggcga agcgactgcg cgatattccc atggtcctgt
     9001 atcgccacaa ccttctgccc cccacgggta atccgctgaa ggcgtggcgt caccgtcgcc
     9061 gggcggcgct gttccacggc catgtcttcg ttagcgagat cggccgcaag caattcgccg
     9121 atcagtatcc gcatctggcc gagcgtgcgg tcagcatcac caacgccatc gacccggctt
     9181 tgtggaaagc gcccgtcgcc ggtcgagcgc ccataatcgc atttgcgggt cgggccgctc
     9241 ctgagaaagg gctcgctccc ctctgtctgg cattggcgac tgcgcttgac gctgcgcctg
     9301 attggcgagc cgaactggcg ctgtccgact ggagcacgca tcaagcatgg agcgaggctc
     9361 aggtcgcgcc gctggcccgg ttcggcgggc gggtcaaaat cgtccacaat gctccgcacg
     9421 atgcggtacg cggcctgttc cagcgcgccg ccatcgtcgc cattccttcg ctgtggcaag
     9481 agccgttcgg actggtcgcg atcgaggccc atgccgccgg cgcggccgtg ttgagctcgg
     9541 gcctcggagg cctcgccgaa gccagcggtg gccacgcgct tgtcgtgacc cctgaggcag
     9601 atttcgctga gcgtctggca gaaggtctgc tctggctggc cgaacatcct gtcgaacggg
     9661 aggctctggc tcaggccggg caagtttaca tcaccaccgc gcacgacgcc cacgaccgat
     9721 cgcaacagct tgatgcaacc cgcaggaact tcatggaggt ctcgcggata cggctctgat
     9781 cgcatcctgt cttcccggcg acacggtcga agcgtagcgt tgggtttatg gccgacggaa
     9841 caccgatttt tcgcccgtgg acacctcctc gacagaccaa gcctcttggg ctgatcccgt
     9901 tcctttgggt aagttggcgc gacccgctgc tgatgtggtc ggaacgccac ttcaaggagc
     9961 cgcaactgtt cggcagcggc cggttcggcg agatcatcgt cgtcagccat cccgcaggcg
    10021 tgcgtcacgt tctgaccgaa aacgccgcca actatgaaaa gggcgcgcta cagcgccggg
    10081 ttctggggcc gatgctggcc gatggcttgc tgctgaccga gggtgatgtc tggcgtcgcg
    10141 cgcgccgtat tctcgcgccc ctgttcaccc cggcccggac gctgaagatg gccgcgcgga
    10201 tggacgccgt gtgccgggcg cgtgttgagg cgctgcatct ggaccatggc gcacgggtgc
    10261 tgaacatcga ctcggagatg tccggcctga cgttcgacat cctgtcggcg accctgttct
    10321 cggacgaact ggccggggac gcacggggtt tcgaacgggc gctgaaccaa tatctgtcag
    10381 tcggcggccg gatcgacccg cttgacgtga tcaacgctcc aggctggatc ccgaggttag
    10441 gtagaacggc gagctatcgc tcggccaagt tcttcgaaca gcgggttgga gcgctcgtgg
    10501 cccgtcggcg agcacggatc gaagcgggcg acgacacgcc cgacgatctg ctgaccgcct
    10561 tgctcacagc gcgagacgaa ggcgaggacg gcaagggact ggacgaccgg gaggtggcgg
    10621 cgaacatcct gaccttcatc cttgccggtc acgaaaccac cgcccgcact ctcggctgga
    10681 ccctgcacct gatctcccgc agtccggaga ctgctacgcg attgaagacc gaagcggacg
    10741 ctttcgacgt caccgacccc aaatgggcgg agggccttcc gtggatgcgc gcggtgatcg
    10801 aggaggcgat gcggctgttt ccacccgccc ccaccctcgc gcggcgcgca ttgggcccgg
    10861 acatcatcgg cggtcaggcg atcagcacgg gcgcagcggt cgtcatctca ccctggcttc
    10921 tgcatcgtca cg
//
LOCUS       DASVTM010000317         6535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_723236,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000317 DASVTM010000000
VERSION     DASVTM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723236"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VF633_08860"
     CDS             <1..525
                     /locus_tag="VF633_08860"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285776.1"
                     /note="catalyzes the formation of
                     2-dehydro-3-deoxy-D-octonate 8-phosphate from
                     phosphoenolpyruvate and D-arabinose 5-phosphate in LPS
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX8471207.1"
                     /translation="DVVQLPAFLCRQTDLVVATARATAAAGGWLHVKKGQFLAPWDSA
                     NIIKKAREAAPDLDIIMCERGVSFGYNNLIVDMLGISQMQNLGVPVTIDATHAVQLPG
                     ADPRTSGGSTGGRREGVAIIAKSAVAAGADGVFLEFHPDPDKALCDGPSCLPVTAAYG
                     LLSTLKTIHEAVRA"
     gene            complement(560..1711)
                     /locus_tag="VF633_08865"
     CDS             complement(560..1711)
                     /locus_tag="VF633_08865"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8471208.1"
                     /translation="MSWTAISTRNETPPISRGGWLDALRFIVASLIILHHFQAAGPVP
                     LADLINPVFQRGGFLLTNFFLIDSGYVLMRVYGASVNAGRMSPGEFFLKRLLRVYPAH
                     LMMGLTLVVLIGVSTALGAPPRNPEWFRWDQLPAQLTLTQAFGVYGGLGWNAPSWSIS
                     ALIGCYLAFPWILKAMKRLGPWSALATVVGLYLLANQATWALFGYPVYQMPMMYGIWR
                     ALPLFILGMGLASFSERVWVAPKLASVVGIAAAVTLAGVQLFDKHALVSLGLISLIIL
                     AAGAVPVVRPSKLIERLSVMSFSMFITNEVTRIAWFGVANVLIARFALPVGVQWGLFV
                     GGVIAAFVVAFLFHTVVDQPMQTRIKTDLKARRTRRMKPGAPVAATELA"
     gene            complement(1796..2368)
                     /gene="folE"
                     /locus_tag="VF633_08870"
     CDS             complement(1796..2368)
                     /gene="folE"
                     /locus_tag="VF633_08870"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX8471209.1"
                     /translation="MTDTPISAAEAEAAVRTLIKWAGDDPDREGLLDTPARVARSYKE
                     LFEGYESDPRAYLERTFEEVGGYNQLVVLKDIRFVSFCEHHMLPVVGVAHVGYLPTDR
                     VVGISKLARVVRGYAKRLQIQEKMTAEIATAIEEVLRPQGVGVVIEAEHSCMTLRGVN
                     VPGAKLTTSALLGVVKDDPRTREEFLRLTR"
     gene            2471..2737
                     /gene="fliQ"
                     /locus_tag="VF633_08875"
     CDS             2471..2737
                     /gene="fliQ"
                     /locus_tag="VF633_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX8471210.1"
                     /translation="MMNGAEVLDVGRDALWLTIQLCAPILIVGLVVGVGIGLFQALTQ
                     IQEQTLVYAPKIIAIFISLLLFLPLMGALLGGFMRTIAARIAGM"
     gene            2745..3527
                     /gene="fliR"
                     /locus_tag="VF633_08880"
     CDS             2745..3527
                     /gene="fliR"
                     /locus_tag="VF633_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX8471211.1"
                     /translation="MEPYATADQVWAGGLIFARVGALLLTMPGIGESYVPPRIRLALA
                     LLMSLVLWPLVAGSLPPLPSTVGGTAGWIIREVATGLMIGAMLRIFTSALSTAGEIVS
                     LQTTLSFAQTANPLSGQQNTTVASFLMLFGTVLVFATNTHHLFIAGIVGSYELIAPAK
                     PLIVADFAQMAVRTVGDSFMLGIQLAAPVIVFALIFNLASGLVARVMPQFQVYFAAAP
                     LSVILGLSIFALSLGVLGTVFIDRFRALASVFAAGAGGVVGG"
     gene            3520..4596
                     /gene="flhB"
                     /locus_tag="VF633_08885"
     CDS             3520..4596
                     /gene="flhB"
                     /locus_tag="VF633_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEX8471212.1"
                     /translation="MADTDPDSKTEDATPQKLEEARRKGDVAKSPDVAQTLSLMGAAA
                     VVIFGGGYFATRMAEAFVPFIASPHAMLGVIDSGSGAQIGMTALWAVAPFMGAVMFAT
                     ILGGVGGNLGQMGGLIFSPSKLAPKWDKVSPMAGFKRLFGPDNFMHFGQTLAKLIAVG
                     VICWMVIKPHMREFENLAGMSPFAILPLTRDLAIALLSSALVFLGGAAGADFIWQKIR
                     FAKRMRMTKEEVKEEFKQSEGDPHDKAKLKQIRMQKSRQRMMANVPKATVIITNPTHY
                     SVALRYEPDQGDGAPICVAKGVDTLAMRIREVAKEHNVPIVENVPLARALYAAVNVDE
                     TIPREHFEAAAKVIGFVMQKRKKR"
     gene            4643..>6535
                     /locus_tag="VF633_08890"
     CDS             4643..>6535
                     /locus_tag="VF633_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8471213.1"
                     /translation="MTALPSAKSQRRFEPLMMVMAVGFAVAIGALAWPAFQAGPTTTP
                     GMILLLTVAAVALIGLFAFGRVDAKRPAGDVAVEMLEAMAEPAALVWAGGQVLAYNSA
                     WASQNGATVALPKGKSAQALYMAFAQARKGEQGRAIVHLGEREIEVLIGQAGPLADGG
                     GRFLVREAPEAALTSHAATTPKAYVAVSGEGRAMAAGAPFGSAVIAGDDIFAARAEDA
                     NTALSVLTGPNAAGDAAFGHLFEPNGVAEARNRLADGSTGPIELVARSFPDRMLHLYV
                     APEGDKRRVWLFDVSAQKSMELQLSQAQKMQAVGQLAGGVAHDFNNLLTAIQLQLSGL
                     LERHPVGDPSYEGLNEIRQTAIRAADLVRKLLAFSRKSTVRRERLDLGELVSEFTVLL
                     RRLLREDVRLETDYGRDLPVVLADKSQLETAVMNLAVNARDAMRGVVAPGAGVVTIKT
                     QRLTVDEARTLGWRDAPADEQGGGEIALIEVSDTGPGVPPELLDKIFEPFFTTKAVNE
                     GTGMGLATVYGIVQQAGGHIGIANIEGAGAAFRIILPAASAAELVEAAPVVAKVKAAP
                     RDLSGAGRILFVEDEAAVRGIAARLLRQRGYEVIEAADGEEALGLAEEWAGQIDMMIS
                     DVIMPGL"
BASE COUNT         1155 a         2140 c         2094 g         1146 t
ORIGIN      
        1 gacgtcgtcc agctgcccgc cttcctgtgc cgccagaccg atctcgtcgt cgccaccgcg
       61 cgcgccaccg ccgccgccgg cggctggctg cacgtcaaga agggccagtt ccttgcacct
      121 tgggacagcg ccaacatcat caagaaggcc cgcgaggctg cgcctgatct cgacatcatc
      181 atgtgcgagc gtggggtgtc gttcgggtac aacaatctga tcgtcgacat gctgggcatc
      241 agccagatgc agaacctcgg cgtgcctgtc accatcgacg cgacccacgc ggtgcaactg
      301 ccgggcgccg atccacgcac cagcggcggc tcgaccggtg gtcgtcgtga aggcgtggcg
      361 atcatcgcca agtcggcggt ggccgccggc gccgacggcg tcttcctcga gttccacccg
      421 gatcccgaca aggcgctttg cgacggcccg agctgcctgc ccgtgaccgc cgcgtacggc
      481 ctgctctcga ccctgaagac gatccacgag gcggtgcgag cctagcgggg gctcgcctat
      541 ggtttggctt taaaaccgct caagccagtt ccgtagcagc gacgggagcg ccaggcttca
      601 tgcgtcgggt cctgcgagct ttcaaatccg tcttgatccg ggtttgcatc ggctgatcga
      661 cgacggtgtg gaacaggaag gccacgacga atgcggcgat gacgccgccg acgaacaggc
      721 cccactgaac tccgacgggc agcgcgaacc gggcgatcag gacgttggcg actccgaacc
      781 acgcgatgcg cgtgacctca ttggtgatga acatcgagaa ggacatcacc gacagacgct
      841 cgatgagctt cgaaggccga acgaccggaa ctgcgcccgc ggccaagatg atcagagaga
      901 tgagccccag cgaaaccagc gcgtgcttgt cgaacagctg gacgccagcg agcgtcacgg
      961 cggccgcgat gccgaccaca ctcgcgagct tcggcgcgac ccatacccgc tcgctgaaac
     1021 tggcaagccc catgcccagg atgaacaacg gcagggcgcg ccagatgccg tacatcatgg
     1081 gcatctgata gacgggatac ccaaacaggg cccacgtcgc ctgattggcg agcagataga
     1141 ggccaaccac ggttgcaagc gccgaccagg ggcccagacg tttcatggcc ttcaggatcc
     1201 acggaaaggc caggtaacag ccgatcagag ccgagatcga ccaactcggc gcattccagc
     1261 caaggccgcc ataaacgccg aacgcctgcg tcagggtgag ttgggccggt aactggtccc
     1321 agcggaacca ctcgggattg cgcggaggcg cgccgagcgc agtcgagacc ccgatcaata
     1381 cgaccagcgt caatcccatc atgagatgcg ccggatagac gcgcagcaac cgcttgagga
     1441 agaattcccc cggcgacatc ctgcccgcat tcaccgacgc gccatagacc cgcatcagga
     1501 cgtagccgga gtcgatcagg aagaagttcg tcagcagaaa accgccgcgc tggaacaccg
     1561 gattgatcag gtccgccaga ggcacgggac ctgcggcctg aaagtgatgc aggatgatca
     1621 ggctggccac aatgaaacgc agcgcgtcga gccacccgcc acgcgaaatc ggcggtgttt
     1681 catttcgggt cgaaatcgca gtccaggaca ttactaaagc tcctaagagc ccgtcgtcgc
     1741 aaagtccgga ccatcggcta ctgcggctgt tgcgaagccc gagagccatg cgtccctagc
     1801 gtgtcaggcg caggaactcc tcgcgcgtgc ggggatcgtc tttcaccacg cctagcaacg
     1861 cactcgtcgt cagcttggcg cctgggacat tgacgccccg cagggtcatg caactgtgct
     1921 cggcctcaat gaccacgccc acgccctggg gccgcagcac ctcctcaatg gcggtggcga
     1981 tttcggcggt catcttctcc tgaatctgga ggcgtttggc ataaccgcgc acgacccgcg
     2041 ccagtttgga gatgcccacc acccggtcgg tcggcaggta tccgacatgg gccacgccca
     2101 cgaccggcag catgtgatgt tcgcagaagc tgacgaagcg gatgtccttc aggacgacca
     2161 gctggttgta acccccgacc tcttcgaacg tccgctccag ataggcgcgc ggatcgctct
     2221 catagccttc gaacagctcc ttgtagctgc gcgcgacgcg ggctggggtg tccagcagac
     2281 cttcgcggtc gggatcgtcg cctgcccatt tgatcagggt gcgaacggcg gcttcggcct
     2341 cggctgcgga aatcggtgtg tcggtcatcg aagccatata gcggtccaaa gtcctgaacg
     2401 ggaggggggg cgaaagtaac cgcccgttaa ccatggaccc ggcattttct gcccggtatt
     2461 gcggagtaga ttgatgaacg gtgcagaagt tctggacgtg ggccgcgacg ccttgtggct
     2521 gacgattcag ctatgcgccc ccattctcat cgtggggctg gtcgtcggtg tgggcatcgg
     2581 cctgttccag gcgctgaccc agatccagga gcaaaccctc gtctatgcac ccaagatcat
     2641 cgcgatcttc atttccctgt tgctgttcct gccactgatg ggcgcgctac tgggcggctt
     2701 catgcgtacc atcgccgctc gcatcgccgg gatgtagcga cgacgtggag ccttacgcca
     2761 ccgccgatca ggtctgggcc ggcggcctca tcttcgctcg cgtcggagct ctgcttctta
     2821 ctatgcccgg catcggcgaa agctatgtgc cgccgcgtat taggctggcg ctcgcgctgc
     2881 tgatgtcgct ggtgctctgg ccgctggttg cgggaagcct gccgccgctc ccgtcgaccg
     2941 tcggcggcac cgcagggtgg atcattcgtg aagtcgcgac cggcctgatg atcggcgcca
     3001 tgctgcgcat cttcacgtcg gcgctttcaa ctgcgggaga gatcgtttcg ctgcagacga
     3061 ccctcagctt tgcccagacg gccaatcccc tgtcgggcca gcagaacacc accgtcgcat
     3121 ccttcctgat gctgttcggg actgttctgg tgttcgcgac caacacccac cacctgttca
     3181 tcgccgggat cgtaggttct tacgagctga ttgcgccggc caagcccttg atcgtcgcag
     3241 acttcgccca gatggccgtg cgcaccgtcg gcgacagctt catgctcggg atccaattgg
     3301 cggcgccagt gatcgtgttc gccctcatct tcaacctcgc ttcgggtctg gtggcccggg
     3361 tgatgcccca gtttcaggtc tatttcgccg cagcgccttt gagcgtcatt ctgggtctgt
     3421 cgattttcgc cttgtcgttg ggtgttctgg gcaccgtgtt catcgaccgt ttccgcgccc
     3481 tggcgtcggt gttcgcagct ggcgcgggag gcgtggtcgg tggctgacac agatcccgac
     3541 tccaagacag aagacgcaac ccctcagaaa ctcgaggagg cgcggcgcaa gggtgatgtc
     3601 gccaagtctc cggacgtcgc ccagaccctg agcctgatgg gggccgccgc cgtggtgatc
     3661 ttcggcggcg gctatttcgc cacccggatg gccgaggcct tcgtgccgtt catcgcctcg
     3721 cctcacgcca tgctgggtgt gattgactcc ggctccgggg cgcaaattgg catgacggcc
     3781 ctttgggcag tcgcgccatt catgggggcg gtgatgttcg ccactatatt gggcggcgtc
     3841 gggggcaatc tcgggcagat gggcggcctg atcttcagcc cctcgaagtt ggcgcccaaa
     3901 tgggacaagg tcagcccgat ggccgggttc aaacgcctct tcggcccgga caacttcatg
     3961 catttcggcc agacactggc caagctgatc gctgtcggcg tcatctgctg gatggttatc
     4021 aagccgcaca tgcgcgagtt cgaaaacctg gctggaatga gccccttcgc catcctgccc
     4081 ctgacgcgcg acctcgcgat cgcgctgctg tcatccgccc tcgtctttct cggaggggct
     4141 gcgggcgcgg acttcatctg gcagaagatc cgcttcgcca aacggatgcg gatgaccaag
     4201 gaagaggtca aggaagagtt caagcaatcc gagggcgacc cgcacgacaa ggccaagctg
     4261 aagcagattc gcatgcaaaa gagccgccag cggatgatgg ccaacgtgcc caaggctacg
     4321 gtgatcatca ccaacccgac ccactattcg gtggccctgc gctacgaacc cgatcaaggc
     4381 gacggggctc cgatttgcgt ggccaagggc gtcgatacgc tggccatgcg gatccgggag
     4441 gtggcgaagg agcacaatgt gccgatcgtc gagaatgtgc ctcttgcccg cgctctctac
     4501 gccgccgtga atgtcgatga gaccatcccg cgcgaacatt tcgaagccgc cgccaaggtc
     4561 atcggattcg tcatgcagaa gcggaagaag cgctgatccc gtttcggcgt atgctccgga
     4621 tactgattcg ccgtggggcc ggatgaccgc acttccttcc gccaagtcgc aacgccgctt
     4681 cgagccgctc atgatggtca tggcggtcgg ctttgccgtc gccataggcg cgctcgcatg
     4741 gccggcgttt caggctggtc cgacgacgac gccgggcatg atcctgttgc tgacggttgc
     4801 ggcagtggcc ctgatcggtc tgttcgcctt cggtcgagtg gatgccaagc gcccggcggg
     4861 tgacgtcgcg gtcgagatgc tggaagccat ggccgaaccg gccgcgctcg tatgggccgg
     4921 gggacaggtt ctcgcctaca actcagcatg ggcatcgcag aacggcgcga ccgtcgcctt
     4981 gccgaaaggc aagtcggcac aggcgctgta catggccttc gcgcaggcgc gaaaaggcga
     5041 gcaaggccgc gcgatcgttc atctgggcga gcgtgaaata gaggtcctga tcgggcaggc
     5101 cggtccgctc gcggacggcg gcggccgctt cctggtgcga gaggctccgg aagccgcgct
     5161 gacatcccac gccgccacca cgcctaaagc ctatgtcgcg gtctcgggcg aagggcgcgc
     5221 catggccgcc ggcgcaccgt tcggctcggc cgtgatcgca ggagacgaca tcttcgccgc
     5281 ccgagctgag gacgccaata cggcgctctc ggtgctgact ggcccgaatg ccgcaggcga
     5341 cgccgccttc ggtcatctgt tcgagcccaa cggcgtggcc gaggcccgga accggctggc
     5401 ggatggctcc accggaccga tcgaactggt ggctcgctcg ttccccgacc ggatgctgca
     5461 cctctacgtc gcgccagaag gcgacaagcg ccgcgtctgg ctgttcgacg tctcggccca
     5521 gaagtcgatg gagctgcagc tttcgcaggc ccagaagatg caggctgtgg gtcagctggc
     5581 cggcggcgta gcccacgact tcaacaacct gctcaccgcg atccagctgc agctctccgg
     5641 tctgctggag cgccatcccg tcggcgatcc ctcgtacgag gggctgaacg aaattcgcca
     5701 gaccgccatc cgcgcggccg atctggtgcg caagctgctg gccttctcgc gcaagtcgac
     5761 ggtgcgccgc gaacggctcg acctcggcga actcgtctcg gagtttacgg tcctgttgcg
     5821 ccgccttctg cgcgaggacg tgcggctgga gaccgactac ggccgcgatc tgcctgtggt
     5881 tttggccgac aagtcgcagc ttgaaactgc ggtcatgaac ctcgccgtca acgcccgcga
     5941 cgccatgcgg ggcgtggtcg ctcccggcgc cggcgtcgtg accatcaaga cccagaggct
     6001 gacggtagat gaggcccgca cgctcggttg gcgcgatgct ccggctgacg aacagggcgg
     6061 cggcgagatc gccctgatcg aagtctccga taccggaccc ggcgtcccgc ccgagctgct
     6121 ggacaagatc ttcgagccct tcttcacgac caaggcggtc aatgaaggca ccggcatggg
     6181 cctggccacc gtctacggca tcgtgcagca ggccggcggc catatcggca tcgccaatat
     6241 cgaaggcgcg ggcgccgcgt tccggatcat cctgcccgcc gcgtcggccg ccgaactggt
     6301 cgaggccgcg cccgtggtcg ccaaggtcaa ggccgcgccg cgcgacctgt caggcgccgg
     6361 ccgcatcctg ttcgtcgagg acgaggcggc cgtgcgcggg atcgccgccc gcctgctgcg
     6421 ccagcgcggc tatgaggtca tcgaggccgc cgacggcgag gaggctctgg gcctggccga
     6481 ggaatgggcg ggccagatcg acatgatgat ctcggacgtc atcatgcccg gccta
//
LOCUS       DASVTM010000318         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_135204,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000318 DASVTM010000000
VERSION     DASVTM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135204"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VF633_08895"
     CDS             <1..394
                     /locus_tag="VF633_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX8471214.1"
                     /translation="TTQEGLGQTTAVGIGGDPVKGTEFIDMLEMFLADDETTSIIMIG
                     EIGGGAEEEAAQFLIDEAKKGRKKPMAGFIAGRTAPKGRTMGHAGAVVSGGKGDAESK
                     IAAMEAAGIRMSESPARLGKTLVEVLKG"
     gene            738..>2458
                     /locus_tag="VF633_08900"
     CDS             738..>2458
                     /locus_tag="VF633_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8471215.1"
                     /translation="MADDAGRLNQVFAETSFLYGANAAFIEDLHEKWASDPASVSAEW
                     RSFFDQLKDSAATVKASGAAGAWGRGAVTEPTDETGVFDGMWPAAKPDPKRAGAAPAK
                     TAPTPTPAAAPTPAGVSVEEVRAAAHDSIRALMLIRTYRVRGHLHAKLDPLGIEQPID
                     NPELTPEFHGFTANDLDRPIYLDGVLGLQYATVRELMAILERTYCGHIGIQYMHIAEP
                     EEKAWLQQRFEGSDKFEQNGFTKEGKLAILNKLIEAEGFERFLHKRFPGTKRFGLDGG
                     EAMVPALEQMIKRGGALGVDEIVIGMAHRGRLNTLAAVMGKPYKAIFHEFQGGSTVPS
                     DIEGSGDVKYHMGASSDREFDGNHVHLSLTANPSHLEIVNPVVLGKARAKQAFDIREA
                     NAGVPEGQWAMDRSKVMPLLIHGDAAFAGQGVVAECFALMGLKGYRTGGTMHFVINNQ
                     IGFTTSPRNSRSTPYCSDVALMVQAPIFHVNGDDPEAVVFAAKVATEYRQKFKKDVVV
                     DMFCYRRFGHNEGDDPTFTQPIMYAKIRSLPSTREIYSKRLVEEGVITQAEVDAELAR
                     FEAWLDE"
BASE COUNT          472 a          791 c          781 g          414 t
ORIGIN      
        1 gaccacgcag gaaggcctag ggcagacgac cgcggtcggc atcggcggcg acccggtgaa
       61 ggggaccgag ttcatcgaca tgctggagat gttcctggct gatgacgaga cgacctcgat
      121 catcatgatc ggtgaaatcg gcggcggcgc ggaagaagaa gccgcgcagt tcctgatcga
      181 cgaagccaag aagggccgca agaagccgat ggcaggcttc atcgcaggtc gtacggctcc
      241 caagggccgc accatgggcc acgccggcgc tgtggtttca ggcggcaagg gcgatgcgga
      301 atccaagatc gccgccatgg aagccgccgg catccgcatg tcggaatcgc ccgctcgcct
      361 cggcaagacg ttggtcgaag tgctgaaggg ctaagcttgc ccttcagcga gcgctccgga
      421 aggagcgctg cgctaacgtt cggctcgcgg agcctcactg cttgagcgca agttcggggg
      481 tgaggttcga tttcaggcag cggtgaattg tcgctgcggc gaccgtcaac ggcgcccgtt
      541 tcggcatcga tcgtgcgctg tacggacatt gaagacgccc tcggctcagg ccgggggcgt
      601 ttttctttgc cgtttgaagc tttttgttac ccgttcgccc cggttcggtc atgacccata
      661 aggaaacagc gcctatatga accgcgctgg cttcacgctg atgtgaccgt ggagccctaa
      721 aaggacgacg agaaacgatg gcggacgatg ccggtcggct gaaccaggtc tttgccgaga
      781 ccagcttcct gtatggcgcg aacgccgcct tcatcgaaga tcttcacgag aaatgggcga
      841 gcgatcccgc ctccgtctcc gccgagtggc gcagcttctt cgaccagctc aaggactcgg
      901 ccgcgacggt caaggcctcg ggcgcagccg gcgcgtgggg ccgtggtgcg gtcactgagc
      961 ctacagacga aaccggcgtg ttcgacggca tgtggcctgc agccaagccg gacccgaaaa
     1021 gggccggcgc cgcgcctgcc aagaccgctc caacgcctac gcccgctgct gctccgacgc
     1081 ctgcgggcgt ctcggtagag gaagttcgcg ccgccgctca cgactccatc cgcgcgctga
     1141 tgctgatccg cacctatcgg gtgcgcggtc acctgcacgc caagctcgac ccgctcggca
     1201 tcgaacagcc gatcgacaat cccgagctga cccccgaatt ccacggcttc accgccaacg
     1261 accttgaccg tccgatctat ctcgacggcg ttctggggct gcagtacgcg accgttcgcg
     1321 agctgatggc gatcctcgag cgcacctact gtggccatat cggcatccag tacatgcaca
     1381 tcgccgagcc cgaagagaaa gcctggctgc agcagcgctt cgaaggctcg gacaagttcg
     1441 agcagaacgg ctttaccaag gaaggcaagc tcgccatcct gaacaagctg atcgaggccg
     1501 aaggcttcga acgcttcctg cacaagcgct tccccggcac caagcgtttc ggcctggacg
     1561 gcggagaggc catggtcccg gctctggagc agatgatcaa gcgcggcggt gcgctcggcg
     1621 tcgacgagat cgtcatcggc atggcccacc gcggccgtct gaacacgctc gccgccgtca
     1681 tgggcaagcc ctacaaggcc attttccacg agttccaggg cggttcgacc gtgccgtcgg
     1741 acattgaggg ttcgggcgac gtcaaatacc atatgggcgc ctcgtcggac cgcgagttcg
     1801 acggcaacca cgtccacctg tcgctgaccg ccaacccgtc gcacctcgaa atcgtcaacc
     1861 cggtcgtgct gggcaaggcg cgcgcgaaac aggcgttcga catccgcgag gccaatgcgg
     1921 gcgtacccga aggtcagtgg gcgatggacc ggtccaaggt catgccgctt ctgatccacg
     1981 gcgacgccgc cttcgcgggc cagggcgtgg tcgccgagtg cttcgcactg atgggcctga
     2041 agggttaccg caccggcggg acgatgcatt tcgtcatcaa caaccagatc ggtttcacga
     2101 catcgccgcg caactcgcgt tcgacgccct attgctcgga cgtcgccctg atggttcagg
     2161 cgccgatctt ccacgtcaac ggcgacgatc cggaagcggt cgtgttcgcg gccaaggtgg
     2221 ccaccgaata ccgccagaag ttcaagaaag acgtggtggt ggacatgttc tgctaccggc
     2281 gcttcggcca caacgaaggc gacgacccga ccttcaccca gccgatcatg tacgccaaga
     2341 tccggtctct gccgtcgact cgcgagatct attcgaagcg cctggtggaa gagggtgtga
     2401 tcacccaggc cgaggtcgac gcagaactgg cgcgcttcga agcctggctc gacgagca
//
LOCUS       DASVTM010000319         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_299074,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000319 DASVTM010000000
VERSION     DASVTM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299074"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF633_08905"
     CDS             complement(<1..435)
                     /locus_tag="VF633_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX8471216.1"
                     /translation="MTSAPTPSPSPAVSSRGLSTLSSDLQFEGSVSGAGDLQVDGLVK
                     GDIRVGRLIVGETGAIEGNVTADYLEVRGRIVGGVNGKQVKLVSTAYVDGDINAEQLS
                     IDIGAYFQGRVLQSRREAPVVAPAPVAAPAAPRLAPTPAADAF"
     gene            complement(521..1669)
                     /locus_tag="VF633_08910"
     CDS             complement(521..1669)
                     /locus_tag="VF633_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8471217.1"
                     /translation="MSEETGAVSRSLIDRLFPERCLYLRERGGEVHGYVLTTGRQMIL
                     AGAAAIVIGWTLVASGGFLFDMINRSQADSQVVRARAASERANADLQARLETAVVRMS
                     ATNGSLDEMAQMVERRHAALTGVMSMFHGVAGAQSALHPAEGLNPRSNSPVQRIMAVR
                     LDQERLIARAETFAQTRAERLRLAFRLAGLNPAAYVVRGAALGGPLVEAKDPRALAAI
                     LDVDEGFAVRIRNASNTLKDMRGLADVADGLPFARPTQARTTSGFGVRFDPFNGRPAL
                     HQGQDFAAQLNTPIYATAPGVISFTGVRSGYGNTVEIDHGHGFKTRFAHLNSIAVQSG
                     QPIALGQRIGAMGTTGRSTGVHLHYEVWVDGRAQNPARFLRAGDQLVQ"
BASE COUNT          359 a          598 c          686 g          334 t
ORIGIN      
        1 gaaagcgtct gcggcgggcg ttggggcaag acgcggcgcg gcgggagctg caaccggagc
       61 cggagcgaca accggggctt cgcggcggct ctgcaggacg cggccctgga aataagctcc
      121 gatgtcgatc gacagctgtt cggcgttgat gtcgccgtcg acgtaggcgg tcgacaccag
      181 cttgacctgt ttgccattga ccccgccgac gatgcggccg cgcacttcga ggtaatcggc
      241 ggtgacattg ccttcgatgg cgccggtctc accgacgatc agacggccga cgcggatgtc
      301 gcccttgact aatccgtcga cctgaaggtc tccggcgccg gataccgatc cttcgaactg
      361 aaggtcggac gacagggtcg acaggccgcg cgacgaaacg gcgggcgagg gcgacggggt
      421 tggggcgctg gtcatcggac gtcctggcga aggcagatcc ggcagaggcg ggatcggcgc
      481 ggaggtcggc cggggctggc cgggtgaagc ggatctggtc ttattgaaca agttggtctc
      541 ctgctctgag gaagcgggct ggattctggg ctcggccgtc aacccacact tcgtagtgca
      601 gatggacgcc tgtcgatcga ccggtagtcc ccattgcgcc gatacgctgg ccgagcgcga
      661 taggctggcc agactggacg gcgatggagt tcagatgagc aaagcgggtc ttgaaaccgt
      721 ggccatggtc tatctcaacg gtgtttccat accctgaacg aacaccggta aaagaaatga
      781 cgcccggcgc cgtcgcatag atcggcgtat tgagctgcgc ggcgaagtcc tgaccctgat
      841 gcagggccgg acgaccgttg aacggatcga accgcacgcc gaagccggag gtggtgcggg
      901 cctgggtcgg acgcgcgaag ggcaggccgt cggcgacgtc ggccagaccg cgcatgtcct
      961 tcagggtgtt cgaggcgttg cggatgcgga cggcgaaccc ctcgtcgaca tcgaggatgg
     1021 cggcgagcgc gcgcgggtcc ttggcctcga ccagcggacc gcctagcgca gcgccgcgca
     1081 caacgtaggc ggccgggttc aacccggcga ggcggaaggc aaggcgcaga cgctcggcgc
     1141 gggtctgtgc gaaggtctcg gcgcgggcga tcagccgttc ctgatccagc cgcacggcca
     1201 tgatgcgctg gacgggggag ttgctgcggg gattgagacc ctcagcaggg tggagagccg
     1261 attgagcgcc tgccacgcca tggaacatgc tcatgacgcc ggtcagcgcg gcgtggcggc
     1321 gctcgaccat ctgggccatc tcgtccagcg agccgttggt ggcggacata cggaccacgg
     1381 cggtctcgag gcgagcctgc aggtcggcgt tggcgcgttc cgaagcggcg cgggcgcgca
     1441 cgacctggct gtcggcctga ctgcggttga tcatgtcgaa caagaagccg cccgaggcga
     1501 ccagcgtcca gccgatcacg atggcggcgg cgcccgccag gatcatctga cggccggtcg
     1561 tcaacacata gccgtgcacc tcaccgccgc gttcgcgcag gtaaaggcac cgttctggaa
     1621 ataatcgatc gatcagggag cgactgactg cccctgtttc ttctgacata tgaacacgcc
     1681 cccacgtcca agcgtcacta tccgtgaaca gtgggcaatc ggcaacaggg ttcacgcaaa
     1741 gttattcaac attgtgcgcc gcacatccca aaacgaatct gtttacggac cagattcgcg
     1801 cgttcggggt attctctacg ggagctgctt tacttccgtc gttgaggtca gcggctcttg
     1861 gttgtgaatt gcgccaagca cagtcgccgc gtgtcccttc accgacacct tgcgccatgt
     1921 gcgctgcaca acgccgtcgt catcgatcag gaaggtggcg cgctcgatgc ccatata
//
LOCUS       DASVTM010000320         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_987172,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000320 DASVTM010000000
VERSION     DASVTM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987172"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VF633_08915"
     CDS             complement(<1..1345)
                     /locus_tag="VF633_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8471218.1"
                     /translation="MDPSSGERLPPNAIRDVVIVGGGTAGWMAAAALIQHLGHTGVRI
                     TVVESSAIGTIGVGEATIPTIRRFYAQLGMTDAEVMTATQASCKLGIRFVGWGEPASD
                     FIHPFGLYGQDLRGVGFHHYWAKQRQTGDPTPLAAYSLGAALARAGRFIEPSPTPPSS
                     LSVFDWALHLDASLFARQLRTYAEARGVQRIDARIDSVELHAEDGFIRALKLDDGREV
                     EGDLFIDCSGFRALLIGEAMGSAYEDWGDWLACDAAFAVQSENAPGMEPAPYTMVTAH
                     SAGWQWGIPLRHRAGNGLVFSSAHMTDQQALDTLMPKLMGKPITEPRRIGFRPGRRDR
                     AWVRNCVALGLSAGFLEPLESTSIALIENGIERLKSLFPDKTFAASVIDEFNDQSARE
                     MERTRDFIILHYWLNGRDEPFWRERRAAEIPDTLARKVELWKARGQFVRYRWEMFH"
     gene            complement(1453..>2428)
                     /locus_tag="VF633_08920"
     CDS             complement(1453..>2428)
                     /locus_tag="VF633_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471219.1"
                     /translation="DTRNYGDTNLDVGDTLSTRTYTDYLPSVNLVYNFSDRLKMHAAF
                     NKTMIPLDLGNYGGGVTIATADSAGPTPTDPTAPPVGIRRVTGATFAGSPDLNPWRSN
                     NYDLSLEYYLGRATMFNIALFKLDIESFVTRGTVPGGRFPDGDGIIRREVPVDMPIQG
                     EGGSLQGVELGAKIALTDFLDDGNFLRNFGFDGSYTFSDSSQTDKNLAGEELPFLDNS
                     EQQLNAAVWYQGEKLQARIAYNYRTPRLANKFGTSGAFIPIYQDTSQYVDVNVTYNVT
                     DNVTVYANGSNIFGEIEEYYLEFEEGAEQFHSRNEFEPRWSLGMRAKF"
BASE COUNT          431 a          745 c          823 g          429 t
ORIGIN      
        1 ggtggaacat ctcccagcga tagcggacga actgtcccct cgccttccag agctcgacct
       61 tgcgcgccag ggtatcggga atttccgccg cgcgccgctc gcgccagaag ggctcatcgc
      121 ggccgttcag ccagtagtgc aggatgatga agtcgcgcgt ccgctccatc tcgcgggccg
      181 actggtcgtt gaactcgtcg atcaccgacg cagcgaaggt cttgtcggga aacagggatt
      241 tcagccgctc gatgccgttc tcgatcaggg cgatactggt gctctccagg ggctcgagga
      301 agccggcgga cagacccagc gcgacgcagt tcctgaccca ggcccgatcg cggcggccgg
      361 ggcggaagcc gatgcggcgc ggctcggtga tcggcttgcc catcagcttc ggcatcaggg
      421 tgtccagcgc ctgctggtca gtcatgtggg cgctggagaa gaccaggccg ttgcccgcgc
      481 ggtggcgcaa ggggatgccc cactgccaac cggcagaatg ggcggtgacc atcgtatagg
      541 gggcgggttc catgcccggg gcgttctcgc tctgcaccgc gaacgctgcg tcgcaagcca
      601 gccagtcccc ccagtcctcg taggcagagc ccatggcctc gccgatcagt agggcccgga
      661 agccggaaca gtcgatgaac aggtcgccct caacctcacg cccgtcatcc agtttgagag
      721 cgcggataaa gccgtcttcc gcgtggagct cgacgctgtc gatgcgggcg tcaatgcgct
      781 gcacgccgcg ggcctcggca taggtcctca gttggcgcgc gaaaagggag gcgtcgaggt
      841 gcaaggccca gtcgaacacc gacaaggacg acggcggcgt cggcgaaggc tcgatgaacc
      901 ggccggccct cgccagggcg gcgcccagcg aataggcggc caaaggcgtc gggtcgccgg
      961 tctgacgctg cttggcccag tagtgatgaa agcccacgcc gcgcagatcc tgcccgtaca
     1021 ggccgaacgg gtgtataaag tcggaggcag gttcgcccca cccgacaaag cggatgccca
     1081 gtttgcaact ggcctgggtc gcagtcatca cttccgcatc ggtcatgccc agttgggcgt
     1141 agaagcggcg gatggtcggg atggtggcct caccgacgcc gattgtaccg atcgcggaag
     1201 actcgaccac ggtgatgcgg acaccagtgt gccccaggtg ctggatcagg gcggcggcgg
     1261 ccatccagcc agccgtaccg cccccgacga tgacgacatc gcggatggcg ttcgggggca
     1321 gtctctcgcc ggacgatggg tccatggccg ttcctcgcct gtctgacgca cgcttcggac
     1381 ctgaaggatt acgcgaaaaa aacggccgac gacaatgcga acgtcgtcgt cggccagggt
     1441 ccagggtggt gactagaact tggcgcgcat gcccagcgac cagcggggct cgaactcgtt
     1501 gcgggaatgg aactgttccg ccccctcctc gaactcaaga tagtattcct cgatctcgcc
     1561 gaagatgttg gacccgttgg cgtagacggt gacgttgtcc gtcacgttgt aggtgacgtt
     1621 gacgtccaca tactgcgacg tgtcctggta gatcgggata aatgcgccag atgtcccgaa
     1681 cttgttggca agacgcggcg tccggtagtt atacgcgatc cgggcctgca gcttttcacc
     1741 ttgataccag acggcagcgt tgagctgctg ctcggagttg tccaggaaag gcaactcttc
     1801 gcccgcaagg ttcttgtcgg tctgcgagct gtccgagaag gtgtagcttc cgtcgaatcc
     1861 gaaattccgg aggaagttgc cgtcgtcgag gaagtcggta agcgcgatct tggcgcccag
     1921 ttcaacgcct tgcaacgaac cgccttcgcc ctgaatgggc atgtcgaccg gaacctcgcg
     1981 ccggatgatg ccgtcgccat ccgggaaacg accgccgggt acggtgcccc gggtgacgaa
     2041 gctttcgatg tccagcttga acagggcgat gttgaacatc gtcgcacgcc ccagatagta
     2101 ctccaggctc aggtcatagt tgttcgaccg ccacgggttc aggtcggggc tcccggcgaa
     2161 cgtggccccc gtcacccgcc ggatgccgac gggcggcgcg gtcgggtcgg tcggcgtggg
     2221 accggccgaa tcggccgtgg cgatggtgac gccgccgccg tagttgccga gatcgagcgg
     2281 gatcatcgtc ttgttgaagg cggcgtgcat cttcagacgg tcgctgaagt tgtagacgag
     2341 gttcaccgac ggcaggtagt cggtgtaggt ccgcgtcgag agggtgtcgc cgacgtccag
     2401 gttggtgtcg ccatagttgc gggtgtcg
//
LOCUS       DASVTM010000321         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_400887,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000321 DASVTM010000000
VERSION     DASVTM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400887"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VF633_08925"
     CDS             <1..906
                     /locus_tag="VF633_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8471220.1"
                     /translation="TKKSPPKTALRGVDLVIPRGSMFGLLGPNGAGKSTLINILAGVV
                     NKTGGTAAIWGRDIDKQPRDARAALGVVPQEIVADVFFTPREALEVQAGFYGVPAKER
                     RSDELLAALGLTDKAHAYVRALSGGMKRRLMVAKALVHNPPILILDEPTAGVDVELRR
                     QLWEYVRRINAEGVTILLTTHYLEEAQELCDTIAIMNRGEVVACEPTPQLLRRLDTRN
                     VVVTPEGELNGLPVIPGFPATARPNGAFAVSYKKGQSSVEQVLAAVRAAGVTIADITT
                     EDPDLEDVFLALTYGDANAVSPTKD"
     gene            complement(994..1407)
                     /locus_tag="VF633_08930"
     CDS             complement(994..1407)
                     /locus_tag="VF633_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HEX8471221.1"
                     /translation="MNRRALFSLAPLAGVALAAAALPALASEGGEGGGAPKETFMPLG
                     TVTATIIRPDGRRGVMTVETGIDIADPELNKQALLQTPRLIAAHNDVVQRTASGMRPD
                     QVPDLDRLAHDLQVATFLVLRKRGAVVLLGTVMIN"
     gene            complement(1442..>2152)
                     /locus_tag="VF633_08935"
     CDS             complement(1442..>2152)
                     /locus_tag="VF633_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEX8471222.1"
                     /translation="RPTPDDLARSDVKLFTPILAGATVRDRAVACLGALFGVCVTGLI
                     TRVILGVDVDLAMVLAAPLGASAVLVFVVPSSPMAQPWPVIGGNVVSALAGFIAARLI
                     PDPMIAAGAAVALSIAAMSMTRSLHPAGGGSALVAALGGPVFASHGSAFAFMPVGLNA
                     LLVVASGWAFHRFTSHSYPHKADPAPVAPPSVSEADIDAAMDIFGEPLDVSSADLQKI
                     ANLAVAVARGRPRGRGQA"
BASE COUNT          392 a          659 c          762 g          339 t
ORIGIN      
        1 accaagaagt ccccgccaaa gacagctctg cgcggtgtcg atctggtgat cccgcgcggc
       61 tcgatgttcg gcctgctggg tccgaacggg gcgggcaagt cgacgctgat caatattctc
      121 gcgggcgtcg tgaacaagac cggtggcacc gccgccatct ggggccgcga catcgacaag
      181 cagccccgcg acgcccgcgc cgcgctcggc gtcgtgccgc aggagatcgt cgccgatgtc
      241 ttctttactc cgcgcgaggc gctggaggtg caggcggggt tctacggcgt gcccgcgaag
      301 gaacgccgct cggatgagct gctggccgcg ctcggcctga ccgacaaggc gcacgcctat
      361 gtccgggctt tgtcgggcgg gatgaaacgc cgcctgatgg tggccaaggc gctggtgcat
      421 aatccgccga tactgatcct cgacgagccg accgcaggcg tggacgtcga gttgcgtcgg
      481 cagctgtggg agtacgtccg gcgcatcaac gccgagggcg tgaccatcct gctgaccacc
      541 cactatctgg aggaggcgca ggagctctgc gacaccatcg ccatcatgaa ccggggcgag
      601 gtcgtggcct gtgagccgac gccacaactg ctgcgccgac tggatacgcg caatgtagtg
      661 gtgacgcccg agggcgagtt gaacggcctg ccggtgatcc cgggcttccc ggccacggcc
      721 cgtccgaacg gcgcattcgc tgtcagctac aagaagggcc agtcgtcggt cgagcaggtg
      781 ctggcggcag tccgcgccgc cggtgtgacg atcgccgaca tcaccaccga ggatccggat
      841 ctggaagatg tgttcctggc gctaacctat ggtgatgcga acgcggtgag tccgaccaag
      901 gactagggga gggattaggg ctgagggctt agggaagatt agagcggctg tttttcttgc
      961 cctaggcctg agctcgaacc cctaatcccc tcttcagttg atcattaccg tgcccagcag
     1021 cacgacggcg ccgcgtttgc ggaggaccag gaaggtcgcg acctgaagat cgtgggcgag
     1081 gcgatcgagg tcgggaacct gatcggggcg catgccggag gcggtgcgtt ggaccacatc
     1141 gttgtgcgcg gcgattaggc gtggggtttg cagcaacgcc tgtttgttga gttccgggtc
     1201 ggcgatgtca atgccggtct cgaccgtcat gacgccacgc cgaccatctg gccggatgat
     1261 agtggcggtc accgtgccga gcggcatgaa ggtttctttc ggcgcgccac ctccttcgcc
     1321 gccttcggac gccagagcgg ggagggcggc tgcggcgagg gcgacgcctg ccaacggagc
     1381 aaggctaaag agcgcgcgac ggttcatcca accggactag cgcagggcgc ttaaggtcgc
     1441 gttacgcctg cccgcgtcca cgcggccttc cgcgtgcgac ggcgacggcc aggttcgcga
     1501 ttttctgcaa gtcggcggaa ctgacgtcca gcggctctcc gaagatgtcc atggcggcat
     1561 caatgtccgc ctcgctgaca gaggggggcg cgactggtgc aggatctgcc ttatggggat
     1621 agctgtggct ggtgaagcgg tgaaatgccc accccgaagc gaccacgagc aaagcattca
     1681 ggcccacggg catgaaggcg aaggccgagc catgactggc gaagacgggg ccgcccagag
     1741 ccgcgaccaa ggcggagcct ccaccggcgg ggtgcaggct gcgggtcatt gacatggctg
     1801 cgatcgacag agcgacagcc gcgcctgctg cgatcatcgg atccgggatc aggcgggctg
     1861 ctatgaagcc cgcgagggct gagacgacat tgccgccgat gaccggccag ggctgggcca
     1921 tagggctgga cgggacgacg aacaccagca cggccgaggc gcccagcggc gccgccagaa
     1981 ccatggcaag atcgacatcg acgcccagga taacccgggt gatcagcccg gtgacgcaca
     2041 cgccgaacaa ggcgccgagg caggcgactg cccggtcgcg tacggtcgca ccggcaagga
     2101 taggtgtaaa aagtttcacg tctgatcggg caagatcgtc aggcgtgggc cg
//
LOCUS       DASVTM010000322         3967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_936918,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000322 DASVTM010000000
VERSION     DASVTM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936918"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VF633_08940"
     CDS             <1..615
                     /locus_tag="VF633_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8471223.1"
                     /translation="IRIWGMGASGRIDDLRNSSRTAAHTAAAQAAGPSHVTLNGLIFN
                     ATGSIPLYVGPGVTSIAVNGGKFSGRSDSTAVYLDAESAGNVIRDVIFDIATPREKIA
                     IDGSASNAIVGNRFALGRRGGVFLYRNCGEDGVIRHQTPSNNLITDNVFTGASPLRSR
                     TVVVGAREGGRGYCGDDRGWPFGSSLDDGDHATGNIVSRNRMSR"
     gene            complement(612..1616)
                     /locus_tag="VF633_08945"
     CDS             complement(612..1616)
                     /locus_tag="VF633_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8471224.1"
                     /translation="MASSPDHAHDDHDHDHVGHDHGHSQSQTGHSHSGHSHAGHGHGG
                     HSHGPVDTGDWRYGVGLLVNLAFVAIEFGAGVFSNSTALMADAGHNLSDVLGLAMAGG
                     AAVLARRAAGKQLTYGFGKATVLAALANALLLIFACGAIAFEAVQRLAEPAPVHSTVV
                     MIVAGIGFFINLGTALLFMRDQHSDLNAKGAYLHMMSDAAVSIGVVISGAIIMFTGWS
                     ILDPLVSIGIVVVILIGTWGLLKDSVNLALDAAPTSVDVGAVKAAFLALPGVSAVHDL
                     HIWGMSTTETALTAHLVHDRVDGETLLAEAQKLARSRFSIGHTTLQLESGLMADCPDC
                     "
     gene            complement(1625..2290)
                     /locus_tag="VF633_08950"
     CDS             complement(1625..2290)
                     /locus_tag="VF633_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471225.1"
                     /translation="MTPPTLEHDVLFSLIGGGFVAAFLHAALPTHWLPFVLVGRAQKW
                     RLPKLLSAVATAGLAHIATTAVVGGLIVAAGLALDQWVEGLMPHLSAILLFAFGGFYL
                     LKAIVRRPAPVTANGATMELTEPSVSHAAAFWGLVAMLAISPGEVLLPIYMSSAQEGI
                     AALALLTVAFALGTILGMGVFTTLARAGASVLKLERWARYEGAVLGIALLGLGLLVLL
                     HQH"
     gene            complement(2332..3795)
                     /locus_tag="VF633_08955"
     CDS             complement(2332..3795)
                     /locus_tag="VF633_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8471226.1"
                     /translation="MTRLLALAATTLGLLFTGGAAHASAPVYAVKACPADWPVQTARV
                     VECGTLTVDETRDDPNTRRIDIAVAVVKASAPYKDASGQALPPVVVFHGGPGGDLVTS
                     VGRRLAFYAANPERVGPLAAIDQDWIYFDQRGGGLGNPSMGCPGAQLTDAGPPSKEDA
                     DKLIACLKGYAAAGVNLSQYNAAGIAADVADLKSAMHLDRIDLFGGSYGPRIEAAVIT
                     HAPQGVRAVVMDSPWPPEGNWAVHTPEQVSAAVKIILAKCAAQTECGARHPNLQTRFE
                     ANARKWLAGPQANTDGKTYTVEDLASFLMDTTYSGSGVRSLPADLEKIIAGDLTAVAE
                     SAEGRTYYTEGQHMTHLCKEELPFESKAALAAGAAGDPVAEVSVPSLSRLFDVCAAID
                     VGVADPVENAPVHTDIPTLFIAAEIDPGCPPPLTRKAAEGYANSQVMIVTNATHGVTG
                     QNPCAARAARDFLRDPTKPVDQSCLPAAGTPLVFAKE"
BASE COUNT          719 a         1244 c         1352 g          652 t
ORIGIN      
        1 atccgcatct ggggcatggg tgcgagcggg cggatcgacg acctgaggaa ttcgtcacgc
       61 acagcagctc atacagcggc ggcgcaggcg gcaggacctt cgcatgtcac attgaatggt
      121 ctaatcttca acgcgacggg ttcgatcccg ctctatgtcg ggccgggcgt aaccagcatt
      181 gccgtcaatg gtgggaagtt cagcgggcgc tccgattcga cagccgtcta tctggatgcc
      241 gagagcgcgg gaaatgtcat tcgcgacgtg atcttcgaca tcgcaacgcc gcgcgagaag
      301 atcgcgatcg atggttcggc cagcaacgcc attgtcggca accgttttgc gctcgggcgt
      361 cgcggcggcg tcttccttta tcgcaactgc ggtgaggatg gcgtgatccg gcaccagacc
      421 ccgtcgaaca acctgatcac cgacaacgtc ttcaccggcg cttcaccgtt gagatcgcgg
      481 accgttgtgg tcggagcgcg cgagggcggc cggggttatt gcggcgatga ccggggctgg
      541 ccgttcggct ccagcctgga cgatggcgac cacgcgacag gcaatatcgt ctcgcgcaat
      601 cgcatgagcc gttagcagtc ggggcagtcc gccatcagtc cgctctcgag ctgaagcgtg
      661 gtgtggccga tcgaaaaccg gctgcgagcc agcttctgcg cttccgccag taacgtctcg
      721 ccatcgaccc gatcgtgcac gagatgggcg gtcagcgccg tttcggtcgt ggacatgccc
      781 cagatgtgca ggtcgtggac ggccgacacc cctggcaggg ctaggaaggc ggccttgacg
      841 gcgccgacgt ctacggatgt gggagctgcg tcgagcgcca ggttgacgga gtccttcagc
      901 agtccccagg tcccgatcag gataacgacg acgatgccga tcgacaccag cggatcgagg
      961 atcgaccagc cggtgaacat gatgatcgcg ccggagatca cgacgccgat cgatacggcg
     1021 gcgtccgaca tcatgtgcag ataggcgccc ttggcgttca ggtcggaatg ctggtcgcgc
     1081 atgaacagca gggcagtgcc gaggttgatg aagaagccga tgccggcgac gatcatcacg
     1141 acggtggaat ggacgggcgc gggctcggcc agccgctgga cggcttcgaa cgcgatggcg
     1201 ccgcatgcga agatcagcag cagggcgttg gcgagcgccg ccagcacggt cgccttgccg
     1261 aaaccgtagg tcagttgttt gcctgccgct ctccgcgcca gaacggccgc gcctccggcc
     1321 atggccagac cgagaacgtc ggacaggttg tggcccgcgt cggccatcag ggcggtggag
     1381 ttgctgaaca ccccggcgcc gaactcgatg gccacgaagg ccaggtttac gagcagtccg
     1441 acgccatagc gccagtcgcc ggtgtctaca ggcccgtgag agtggccgcc gtggccgtgc
     1501 ccggcgtgcg aatggccact gtgggaatga ccggtctgcg attgggaatg gccgtggtcg
     1561 tgaccgacgt gatcatggtc atggtcatcg tgcgcgtggt cgggagagga agccatgccc
     1621 cctcttagtg ctgatgcagc aggacgagaa ggcccagccc cagaagggcg atacccagca
     1681 cggcgccctc atagcgggcc cagcgctcca gcttgagcac cgaagcgccc gcccgggcca
     1741 gcgttgtgaa gacgcccatg ccaaggatgg tgccgagggc gaaagcgacg gtcagcagcg
     1801 cgagcgcggc gatgccctcc tgcgccgagg acatatagat cggcagaagc acctcgccgg
     1861 gagaaatggc cagcatggcg accagtcccc agaaggcggc tgcgtgcgac acgctaggtt
     1921 ccgtcagctc catcgtcgcg ccgttggccg tgactggcgc gggcctgcgg acgatggcct
     1981 tgagcaggta gaagccgccg aacgcgaaca gcaggatggc cgacagatgc ggcataaggc
     2041 cctcgaccca ctggtccaat gccagacccg ccgccacgat caggccaccg accacggccg
     2101 tggttgcaat atgggcgaga cctgccgttg ctacggcgct tagcagcttg ggcagtcgcc
     2161 atttttgcgc ccgcccaacg aggacgaacg gcagccagtg cgttggcagt gcggcatgca
     2221 gaaatgccgc cacgaagccg ccgccgatca gcgaaaacag aacatcatgc tcgagggtcg
     2281 gcggcgtcat cggcagcgat ctatcgtgtt attttataac agtcgagagt tttattcctt
     2341 ggcaaacacc agaggtgttc cggccgcagg cagacagctt tgatcgaccg gcttcgtcgg
     2401 gtcgcgcagg aaatcccgag cggcacgggc ggcacagggg ttctggccgg taacgccgtg
     2461 ggtggcgttg gtgacgatca tcacctggct gttcgcatag ccctcggcgg ccttgcgggt
     2521 caggggaggc gggcagccgg gatcgatctc ggcggcgatg aacagggtcg ggatgtcggt
     2581 atggaccggg gcgttctcga ccggatcggc cacgccgacg tcgatcgcgg cgcagacgtc
     2641 gaacaaacgc gacaggctgg ggaccgagac ctcggcgacc ggatcacccg cggccccggc
     2701 ggccagggcg gcctttgact cgaacggcag ctcttccttg cacaggtggg tcatgtgctg
     2761 gccctcggta tagtaggtgc gcccttccgc gctttcagcc acggcggtca ggtcgccggc
     2821 gatgatcttc tccagatcgg ccggcaggct gcggacaccc gaaccggaat aggtcgtgtc
     2881 catcaggaac gaggccagat cctcgacggt gtaggtcttg ccatccgtat tagcctgcgg
     2941 accagccagc catttgcgag cgttggcttc gaaccgtgtc tggaggttgg ggtggcgcgc
     3001 gccgcactcc gtctgggctg cgcatttggc gaggatgatc ttcacggcgg ccgagacctg
     3061 ctccggcgtg tggacggccc agttgccctc cggtggccag ggcgagtcca tcaccacggc
     3121 gcggacgccc tgcggcgcat gggtgatgac ggccgcctcg atgcgcggac cataggagcc
     3181 accgaacagg tcgatccggt cgagatgcat ggccgacttc aaatcagcga catcggcggc
     3241 gatcccggcg gcattatact gagacaggtt cacgcccgca gcggcatagc ccttgagaca
     3301 ggcgatcagc ttatccgcgt cttccttgga cggcggcccg gcatcggtca gctgcgcgcc
     3361 ggggcagccc atggacggat tgccgagacc gccgccacgc tgatcgaaat agatccagtc
     3421 ctgatcgatc gcggccagcg gtccgacgcg ctccggattg gcggcgtaaa aggcaagccg
     3481 ccgaccgacg ctggtcacca gatcgccgcc cggtccgccg tggaacacga ctaccggcgg
     3541 cagggcctga cctgatgcat ccttgtaagg cgcgctggcc ttcacgacag cgacggcgat
     3601 atcgatacgg cgtgtgttcg gatcatcccg cgtctcgtcc accgtcaggg ttccgcactc
     3661 cacgacccgc gccgtctgga cgggccagtc tgcaggacag gccttcaccg catagacggg
     3721 ggcgctggcg tgcgccgcgc ctcccgtgaa caacagtccg agcgtagtcg cagcgagagc
     3781 gagcagtctg gtcataggcg atcctccgct ttcaccagac accgtgaaga cggggtgacg
     3841 caagagcgac cgccaccccg attattggga ccgtcaagct ggcttggagc gaggcttctt
     3901 cgggccggtg accttggcga tgacacgcga catgaccgcc ttgacgccgc gcgcgggctt
     3961 cggcgtc
//
LOCUS       DASVTM010000323         5820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_400902,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000323 DASVTM010000000
VERSION     DASVTM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400902"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(499..876)
                     /locus_tag="VF633_08960"
     CDS             complement(499..876)
                     /locus_tag="VF633_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471227.1"
                     /translation="MILNVLHVDDEPDIREVAALALEMDPGVNVRSAASGRDALGVLE
                     SNAQIDVILLDVMMPELDGPGTLEQLRIIPAHADTPVIFMTARAQSHELERFLGLGAL
                     AVILKPFDPMTLGQQIRDHLASA"
     gene            complement(873..3365)
                     /locus_tag="VF633_08965"
     CDS             complement(873..3365)
                     /locus_tag="VF633_08965"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX8471228.1"
                     /translation="MRRVGQALPTAYRWLAIVAAVLAPLLLVFSGFELINEFRSNRIV
                     RAEIDRSYETRSQIQSVFSLLQDAETGQRGYVITGDERFLEPYERATSTLDRQMQALG
                     VLFRHHPGQADDYAKLNDLAARKVAAMEVGIDARQTQGLQAGIAVVSGGEGKRLMDEV
                     RAVVARMNAVEAEALNAYARTAQERTARTELLVGGLFLALTAAIGGASLLIWRYIRTR
                     QTMLSAIEATAARQSAIFDSAIDGIVTLNPSGTIETVNAAAARMFGYEAAEVTRRDMS
                     MLIDIAPDGDGEFLKRLGASQGALEGGLLRQMDARHRNGDTFPVDVALGPMRLPSGIH
                     IVAIIRDISERRRMEQMKDEFVATVSHELRTPLTSIAGSLGLLAGGAAGALPDKAARL
                     IQIAHANSQRLVRLINDILDIEKIESGKLRLDMAPLDLREIAQRSLEGVGGYAGDLEV
                     KLTLTGQGPVAIRGDADRLIQVVTNLLSNALKFSPRGGEVRVSVDPETRLARLSVVDE
                     GPGISEAFRARIFSKFAQADSSDTRAKGGTGLGLAIAREIAERHGGRLWFESAEGYGA
                     TFHLDLPLVEDSPASADTGGPRLLIVEDDADAAEVLREMLERDGYAADVVATGREALS
                     AARTGAYAALLVDLQLPDADGIGLIRTLRARPETRNLPVVVVSGDTARGMARGRSLEV
                     VDWLEKPFDQARLRTAVAALHQRTKDRKPRILHVDDDRDILDVTASALGGGVDVTSAE
                     SLAAARAVLAGMKPDLVILDLGLPDGSGLELLGDLGDSAGKTIPVVVYSAQEMDGALA
                     DRVEAVLTKSRTSLAGLARTVRRLTDAGGAGK"
     gene            3414..3770
                     /locus_tag="VF633_08970"
     CDS             3414..3770
                     /locus_tag="VF633_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471229.1"
                     /translation="MKVCVIDDDPLMLQYLHDMVAGLGFEVVSAPDVKTAFELMAAQH
                     CDAAVVDILMPDRDGIDFIMQARRVHPGLRIVAISGGGRLGADAVLGMAAGLGADRTL
                     VKPFSASELQMALTAA"
     gene            complement(3792..5234)
                     /locus_tag="VF633_08975"
     CDS             complement(3792..5234)
                     /locus_tag="VF633_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8471230.1"
                     /translation="MLNFEPDLFLAVAAAVIAWFVIGTGLLQNLIYLVQLGLAAAALV
                     ERPPTSQAGVMWRRLSESAPPIALLAPAFNESLTISQSVRSLLSLHYPNFEVVVINDG
                     SSDDTLAVLIETFDLKPIERHYDLEIAHASIRGLYGAVHQPRLIVIDKVNGGKADALN
                     AGINVSRAPIFCSMDADSLLEPDALLRAVSPFVEDPVRTVAVGGTVRIANGCVIADGR
                     VVEVRAPRDPLALLQTVEYLRAFLMARLAWSRINTLTIISGAFGLFRRARVIEVGGYT
                     HGTVGEDMELVVKLHRLMRDNRLPYRIAFVPEPVCWTEAPESLSVLGRQRSRWHRGAL
                     ETFERHWPMLFIPRYGRVGAIGFGYILLVDVLGPIVEALGYLLIPLFWMSGLLSVEYL
                     LAFVAMSFTFGVVISVGSLALEESELRRFPRARDLVLLTLVAVLENFGYRQLNTFWRL
                     RGVWQYLRRSQSWGTMTRKGFGSAGVARGA"
     gene            complement(5228..>5820)
                     /locus_tag="VF633_08980"
     CDS             complement(5228..>5820)
                     /locus_tag="VF633_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8471231.1"
                     /translation="PPPLGRLLDDLEARKMTDSLVYLPIVRRLVALSPDEAMSRLDSS
                     ATSPAARVLLADAVAVTGDYRAVAPLQRAARAERPDLRMAAIRGLSLLAHPASIPTLT
                     EALSDPDWEVRAAACEAMGRTGFNEGVADLADRLADSVWWVRFQAAEALSRLGPGGLE
                     ALLHASRLSDDLPRRAASLALAEKGFAAPVLEHGSC"
BASE COUNT         1098 a         1906 c         1860 g          956 t
ORIGIN      
        1 cgagctctac tccgggccga aagcttcagc aactgatcag agagggcagc aattaccgtg
       61 acggaactac ccgtgccgga cggaaccatc tcacctaacg gcgtgatatt caaccgctgc
      121 cgccgaccat ggctttaacc ggactgcccc atccgctgtt gtttgctagg gcagatccac
      181 accgggcccc agagcgtcga ccaagccgag agcgcaagaa cgctgacggg gtgggacggc
      241 ggaacaggcg gaaaggcagc tgtcgaaact gcccgtcgca tcaggcgtcg aactcattcc
      301 tccaccgatc agcgtcagga tgtgcgccga ggctccggtc tctgtctatg tccaggtcgc
      361 tccggccgac gcatcggcgc tgaagccgct ggtgcgcctg ttcgctggcg agattttccc
      421 gcgccaggcg ttcgagcgcc tcacgagccg ccaatgcgcg atcgcggacg cgggccctga
      481 gcgctgcgaa gggatcggtc acgcgctcgc caggtggtcg cgaatctgct gaccgagggt
      541 catcggatcg aacggcttca aaatcactgc gagggcgcca aggccgagaa atcgttccag
      601 ctcatgggat tgagcccggg ccgtcatgaa gatcacgggc gtgtcagcat gagcggggat
      661 gatccggagt tgttcgaggg tgcccggacc gtctagctcg ggcatcatga catccagcag
      721 gatgacgtcg atctgagcat tcgactccag aacgcccagg gcgtcccggc cggatgcggc
      781 ggatcgaacg ttgacgcccg ggtccatttc gagcgccagc gccgcaactt cacggatatc
      841 gggttcatcg tccacgtgca ggacgttcaa aatcattttc cagctccacc ggcatccgtc
      901 agtctacgca cggtccgcgc caagcctgcg agagaggtcc gcgacttggt caggaccgcc
      961 tcgacccggt ccgccaacgc gccatccatc tcctgcgccg aatagacgac cacagggatc
     1021 gtcttcccag cgctgtctcc cagatcaccc agcagttcca gacccgatcc atcgggcagg
     1081 ccaaggtcga ggatcacgag gtcaggcttc attcccgcca gcacggcccg cgccgcagcc
     1141 agactttcgg cggaggtgac atccacgccg cccccaaggg cggacgcggt aacgtccaga
     1201 atgtcacggt cgtcatcgac gtgcaggatg cggggtttcc gatctttggt gcgctgatgg
     1261 agagcggcga cggccgtgcg cagtcgagct tggtcgaacg gcttttcaag ccagtccaca
     1321 acctccagag accgcccccg cgccataccc cgggccgtat cgcctgagac caccaccact
     1381 gggaggttcc gcgtctcggg cctcgccctc agagtccgga tcagcccgat cccgtcggca
     1441 tccggcaact gaaggtcgac cagcagggcg gcgtaggcgc cggtgcgcgc cgcggacagg
     1501 gcctcgcggc cggtcgcaac cacatcggcc gcatagccgt cacgctccag catctctcgc
     1561 agaacctcgg ctgcgtccgc gtcatcctcg acgatcagca ggcgcggccc ccccgtgtcg
     1621 gccgatgccg gagagtcttc gaccagcggc aagtcgaggt ggaaggtcgc gccgtagcct
     1681 tcggcggact cgaaccacag acgaccgcca tggcgttccg cgatctcgcg ggcgatcgcc
     1741 agccccagac ccgtgccgcc cttggcccgg gtgtcggagc tgtcggcctg ggcgaacttt
     1801 gagaaaatgc gcgcacggaa agcttcggag atgccgggtc cttcgtcgac gacgctcagt
     1861 cgcgccagcc gggtctccgg atcgacgctg acccgcacct cgccgcctcg cggcgaaaac
     1921 ttgagcgcgt tggacagaag attggtcact acctggataa gccggtccgc atccccgcga
     1981 atagcgaccg gcccctgccc ggtcagggtg agctttacct ccagatcgcc ggcatagccg
     2041 ccgacgcctt ccagcgatcg ctgtgcgatc tcgcgcaggt caaggggagc catgtcgagc
     2101 cgaagcttgc cggattcgat cttctcgatg tcaaggatgt cattgatcag cctcaccagt
     2161 cgctggctgt tggcgtgggc gatctggatc agccgcgcgg ccttgtcggg caaggcgccc
     2221 gcagcgccgc cggccagcag tcccaacgac cccgcaatgg acgtgagggg cgttcgcagt
     2281 tcgtggctca cggtcgcaac gaactcatcc ttcatctgct ccatccggcg ccgttcagag
     2341 atgtccctga tgatggcgac gatgtggatg ccggatggca agcgcatcgg gcccagggcg
     2401 acatcgaccg gaaaggtgtc gccgttcctg tgacgcgcgt ccatctggcg cagaaggccg
     2461 ccctccagcg caccctgcga tgcgcccagc cgcttgagga actcgccgtc gccgtcgggc
     2521 gcgatgtcga tcagcataga catgtcgcga cgggtaacct ccgcggcttc atagccaaac
     2581 atgcgtgcag ctgcggcgtt gaccgtttcg atggtgccgc tcgggttgag tgtgacgata
     2641 ccgtcgatgg cgctgtcgaa aatcgcactc tggcgcgcgg ccgtcgcctc aatggcggac
     2701 agcatggtct gccgcgtgcg aatatagcgc cagatcagga gcgatgcgcc gccgatcgcc
     2761 gcagtcaagg ccaggaaaag gcctccgacg aggagctctg tccgcgctgt ccgctcctgc
     2821 gcggtgcgcg cataggcgtt cagggcctcg gcctccaccg cgttcattcg ggcaacaacc
     2881 gcgcgaacct cgtccatgag gcgtttgccc tcgccgccgg aaaccacggc gatcccggct
     2941 tgcagcccct gggtctgacg ggcatcgatt ccgacctcca tcgcggcgac tttgcgcgcg
     3001 gccagatcgt tcagctttgc gtagtcgtcg gcttgtccag ggtggtgccg gaagagcacg
     3061 cccaaggcct gcatttgcct gtcgagagtg ctggtcgcgc gctcgtaggg ctcaagaaag
     3121 cgctcgtctc ctgtaatgac atagccccgc tgcccggttt cggcgtcctg cagcaaggag
     3181 aatacgctct gaatttgtga gcgggtttcg taggatcggt cgatttcggc gcggacgatc
     3241 cggttgcttc tgaactcatt gatcagttca aaacctgaga agaccagcag cagcggcgcc
     3301 agcacggccg cgacaatcgc cagccagcga taagcggtcg gcagcgcttg acctacccgc
     3361 ctcaaggttg cgtaaccttc ccggcgtcag cacaacccct gggagatgat cgggtgaaag
     3421 tttgcgtcat cgacgacgat ccattgatgt tgcagtatct gcacgacatg gtcgccggac
     3481 ttggtttcga agtcgtgagt gctccggacg taaagaccgc attcgagctc atggccgccc
     3541 aacactgcga cgcagcggtc gtcgacattc tgatgcccga ccgcgacggg attgatttca
     3601 tcatgcaggc gcgccgcgtc catcccggcc ttcgaatcgt ggcgatcagc ggcggcggaa
     3661 gattgggggc cgacgcggtt ctgggcatgg cggccgggct cggagccgac aggacgctcg
     3721 tgaagccgtt ctcggccagt gaactgcaga tggctctgac agcggcctga ccggccccgc
     3781 gctggtcgcg ttcaggcgcc acgcgcaacg cccgcagatc caaagccctt acgggtcatg
     3841 gtcccccatg actggctccg cctcagatac tgccagacgc cccgcagtcg ccagaacgtg
     3901 ttgagctggc ggtagccgaa gttttccagc acggcgacga gggtcagcaa gacaaggtcg
     3961 cgggcgcgtg ggaagcgtcg caattcggac tcttcgagcg cgagagagcc caccgagatg
     4021 acgactccaa aggtgaaact catggccaca aacgccagca gatattcgac cgagagcaga
     4081 ccggacatcc agaacagcgg aatcaacaga tagccgagcg cctcgacgat cggtcccaga
     4141 acgtcgacca ggaggatata gccgaaaccg atcgcgccga cccgtccata acgtgggatg
     4201 aagagcatcg gccaatggcg ttcgaacgtc tccagagcgc cccggtgcca gcgggaccgc
     4261 tgccgtccaa gcacgctcag gctttccggc gcttcggtcc agcacaccgg ctcgggtacg
     4321 aaggcgatac ggtaagggag cctgttgtcg cgcatcaacc gatgcagctt gaccaccagt
     4381 tccatgtcct cacccacggt accgtgggtg tatcccccga cctcaatgac gcgggcgcgg
     4441 cgaaacagcc cgaaggcgcc ggaaatgatt gtgagggtgt tgatgcggct ccaggcaagg
     4501 cgcgccatca ggaacgccct caggtattcg accgtctgaa gcagggccag aggatcgcgc
     4561 ggcgcgcgaa cctcgaccac ccgcccatcg gcgatcacac agccgtttgc aatccgaacg
     4621 gtccccccga cggccaccgt acgcacaggg tcctcgacaa agggactcac cgccctcagc
     4681 aaggcatcag gttccagtag tgaatccgcg tccatggaac agaagatggg cgcgcgcgac
     4741 acattgatcc cggcgttcag ggcgtcggcc ttgccgccgt tgaccttgtc gatcacgatc
     4801 agccgcggtt gatggaccgc tccataaaga ccgcggatcg atgcatgggc gatttccaga
     4861 tcgtagtgcc gctcgatcgg tttgagatcg aaggtttcta tcagtacggc cagggtgtcg
     4921 tcgctcgacc cgtcgttgat gacgacgact tcgaagtttg ggtagtgcag cgacagcaac
     4981 gacctgacgg actggctgat cgtcagggac tcgttgaagg ccggggccag aagcgcaatc
     5041 ggcggggcgc tttcggacaa tcgacgccac atcaccccgg cctgggacgt aggcgggcgt
     5101 tcgacaaggg ctgcggcggc caacccgagt tgcaccagat agatgagatt ctgcagaaga
     5161 cctgttccga tcacgaacca ggcgattacg gcagccgcga cagccaggaa caggtcgggc
     5221 tcgaagttca gcacgacccg tgctccagca cgggagcggc aaaccccttt tcagccagag
     5281 cgagggaggc ggctcgacgc gggaggtcat cggagaggcg cgaggcgtgc aagagggcct
     5341 ccagaccgcc cggtcccagg cggctcaggg cctcggccgc ctggaatcgc acccaccaca
     5401 cgctgtcggc gagccggtcg gccagatcgg cgacgccctc gttgaagccg gtacgcccca
     5461 tcgcttcgca agcggcggcc cggacttccc aatcgggatc cgacagcgct tccgtcaagg
     5521 tggggatcga agccgggtgg gcgagcaggc tcagcccccg tatggcggcc atccgaagat
     5581 cgggtcgttc tgcccgcgcg gcccgctgaa gcggcgctac ggcgcgatag tctccggtca
     5641 cggcgacggc atccgcgagc aacacgcgcg cggcgggcga ggtggcggag gagtccagtc
     5701 ggctcatggc ctcatctggc gacaaggcaa ccagacggcg cacgatcgga agatagacga
     5761 gcgagtcggt catcttcctg gcctcgagat cgtccagcag ccgacccagc ggcgggggtc
//
LOCUS       DASVTM010000324         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_723997,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000324 DASVTM010000000
VERSION     DASVTM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723997"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1124)
                     /locus_tag="VF633_08985"
     CDS             complement(360..1124)
                     /locus_tag="VF633_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8471232.1"
                     /translation="MFISSRVPLRRAGPAPVIAHFSALAKLTPPEVEILAGLNDWRWE
                     PAGSELVRDRTILSPRVLVSGWAARVRWLEDGRRQIIGLILPGDGIGLCRRPQPLALC
                     PVLALTPIQTLDAAPIMRAIEGAPDFARLSHAVNVAAAMDEAWLMDQVVRLGRLTAYE
                     RLAHLILEIRARLKALGPDVDGSIPFPLTQEMLADATGLSMVHVNRTLQQLRREKLIE
                     LGHGRLKILDLQALVDLSDFREPEPDAWIFQGQRQL"
     gene            complement(1124..>1591)
                     /locus_tag="VF633_08990"
     CDS             complement(1124..>1591)
                     /locus_tag="VF633_08990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8471233.1"
                     /translation="RLAALASIQATLARTQSGQADLEDIVRQELVHSVGSDEGIEVEG
                     PTILLKGRTVELIALALHELAANAVKFGALSTGGRLAVTWTHAPTAPGSGQNLILSWK
                     ETGVASADPDHAGFGLELIKRGLPYELDADVAVDFTPTGLECTILLPLGRGGA"
BASE COUNT          280 a          529 c          509 g          273 t
ORIGIN      
        1 cccttcgtgt tcgcgacggg ctacgacgcc tcccttctcg tggggttcga gacctcgcca
       61 cggcttgaga aacctctgga cccgcgggcc atggcgtgga cgatttcgaa gttggtcgat
      121 tccggcgacg gaggcagggg ccggccattt gtgtgagggc aggctggcta caggcgaggc
      181 cgatcgccgc cgtcagcgct cactcctcga tgccgccgtc ttgcggcgtt ctgccgcggg
      241 agaacacgga cgccagcccg atccctggat acttgtcccc tcgtcgtcga gaccactgat
      301 ttgagcgaat gtagcagcgc gaaatctttc acgtacgtca cggtcgcgcg ccgagttcgc
      361 tacaactggc gttgtccctg aaatatccag gcgtcgggct caggctcgcg aaaatccgac
      421 aaatcgacca gggcctgaag gtccaggatt ttcaagcgac cgtggccgag ttcgatcagc
      481 ttttcacggc gcagctgctg cagcgtcctg ttgacgtgca ccatgctcag tccggtcgca
      541 tcagccagca tctcctgggt tagcggaaac gggatgctgc cgtcgacatc cggaccaagc
      601 gccttcaggc gggcccttat ctccaggatg agatgggcga gccgttcgta ggcggtcaac
      661 cggcccagcc gcacgacctg gtccatcagc caagcctcgt ccatggcggc ggccacgttg
      721 acggcgtgag acagcctcgc gaaatcaggc gccccctcga tggcgcgcat gatcggcgcg
      781 gcgtccaggg tctggatggg ggtgagggca agcacggggc agagcgccag cggctgcggc
      841 cgtctgcaca gcccgatccc gtcacccggc aagatcagcc cgatgatctg gcgtcgccca
      901 tcctcgagcc acctgacccg cgcggcccat cccgagacca gaacccgcgg cgacaggatc
      961 gttctgtcac gcaccagttc tgaaccggcc ggctcccaac gccagtcgtt cagtccggcg
     1021 agaatctcca cctccggcgg ggtaagtttt gccagtgcac tgaagtgggc gatgacgggc
     1081 gccggccccg cccgacgcaa ggggacgcgc gaagatatga acatcaggcg cccccgcggc
     1141 caaggggaag caggatcgtg cactccagac cggtcggtgt gaaatcgaca gcgacatcgg
     1201 cgtcgagttc atacgggagg ccccgcttga tgagttccag accaaagccg gcatgatccg
     1261 gatcagccga agccacgccg gtttccttcc aactcaggat caggttttga ccgctccctg
     1321 gggcggtcgg cgcgtgcgtc caggtcaccg ccaggcgtcc cccggtcgaa agggctccga
     1381 acttcacggc gttggccgca agctcatgca gggcgagcgc gatcaactcg actgtgcgac
     1441 ccttcaggag gatcgtcggc ccctcgacct caatgccttc gtcggagccc acactgtgga
     1501 cgagctcctg gcggacgata tcttccagat ccgcctgacc gctctgcgtt ctggccagcg
     1561 tcgcctggat gctggcgagt gcggcgaggc g
//
LOCUS       DASVTM010000325         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_777603,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000325 DASVTM010000000
VERSION     DASVTM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777603"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2039
                     /locus_tag="VF633_08995"
     CDS             <1..>2039
                     /locus_tag="VF633_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471234.1"
                     /translation="ITADTIRLLRRPEIEPPDGKPRQPAPISIEIDKFDANVELLEGF
                     SKEYGRWRLTGSADIPRFGAKDATVNAYSLNRPGDYLRLTATFGGKVDELRLNLRANE
                     AQGGPIAGSLGYSPDQPFSAIAVINGEIVDATVKTGAFTPLLIKGRYGKDGARISGYA
                     DFSGSDLLEPFVKRIGRTARFGFAAIPDANRKGFQGVGWRFISENFNSNAQGMIRLSD
                     RSAPDGVKLEVSTQSLTRLLGLTLGGPTAWSGTFNGDAATWSLTGRATMLAADIASYK
                     ATRVTGPLNVQVKDGRMDIDSDLLTNGGSTEGVIGALFGTAPHLKFEAVRMKDGAFLF
                     EKLDLVGQALLVNGTGTRNLLGALGFRGQARITDIGRIQAGATGQLAGAITASNRSGQ
                     PWKLTFDGRGAKVATGLAELDRLLGATPRVQFEGQMERELAIERLLVTGTMGEAAAKG
                     LMGFDGRLRLALNWNARGPFGVGPVAIDGAMTGDGALTGTLAEPRVDLTAGFDKVDAG
                     PLKMLQTKLILSFRKGADASDGRVTITSGSNYGPAAASGSFFLGSKSVRLTDVDVDAG
                     GIKAQGAISLSDNIPSSADLTFSARPGAFITSGSAEGRVRLTEGNGDDTAILAVTARN
                     ARLTGSTITVRSLDLNGRGTLSRLPFTLAMDVGGDNPLQFNGTGVYARQGSGADA"
BASE COUNT          354 a          654 c          702 g          329 t
ORIGIN      
        1 gattactgcc gacaccatcc ggttgctgcg ccgtcccgag attgagccgc cggacggcaa
       61 gccccgccag cccgccccga tcagcatcga aatcgacaag ttcgatgcca acgtcgaact
      121 gctggagggc ttctccaagg aatatggcag gtggcggctg acgggctcgg ccgacatccc
      181 gcgcttcgga gccaaggacg ctaccgtcaa cgcttacagc ctgaaccggc ccggcgacta
      241 cctgcgcctg accgccacgt tcggcggcaa ggtcgacgag ctgcggctca acttgcgggc
      301 caacgaggcg cagggtggcc cgatcgcggg ttcgctaggc tattcgccgg accagccgtt
      361 ctcggctatc gccgtgatca acggcgagat cgtggacgcc acggtcaaga caggggcctt
      421 caccccgctg ctgatcaagg gacgctatgg caaggacgga gcgcggattt cgggatacgc
      481 cgacttcagc ggttcggatc tgctggagcc gttcgtcaag cgcatcggtc gcaccgctcg
      541 cttcggtttc gccgccatcc cggacgccaa ccgcaagggt tttcagggcg tcggctggcg
      601 ctttatctcc gagaatttca actccaacgc ccagggtatg atccgtctgt cggaccggtc
      661 ggcgccggac ggcgtcaagc tagaggtctc gacccagtct ctgacccggc tgctggggct
      721 cacactcggc ggtcccacgg cgtggagcgg cacgttcaat ggcgacgccg cgacctggag
      781 cctgacagga cgcgccacca tgctggccgc cgacatcgcc agctacaagg cgacgcgtgt
      841 gacgggcccg ttgaacgttc aggtcaagga tggccggatg gacatcgaca gtgatctgtt
      901 gaccaacggc ggctcgaccg agggcgtcat cggcgccttg ttcggcacgg cgccgcacct
      961 gaagttcgag gctgtgcgga tgaaggacgg ggcgttcctg ttcgagaagc tcgacttggt
     1021 cggtcaggcc ttgctggtca acggcacggg cacgcgcaac ctgctgggcg cgctgggctt
     1081 ccggggtcag gcgcggatta ccgacatagg tcgcattcag gcgggcgcca ccgggcaact
     1141 cgcgggcgcg atcaccgcct cgaaccgctc gggccagccg tggaagctga cgttcgacgg
     1201 gcgaggagcc aaggtcgcga ccgggctggc ggaactggat cgtctgctgg gcgcgacgcc
     1261 gcgtgtgcag ttcgaaggcc agatggagcg tgagctggcg atcgaaagac ttctggtcac
     1321 ggggacgatg ggcgaggctg cggccaaagg gctgatgggc ttcgatggcc ggctgcgtct
     1381 ggcgctgaac tggaacgcgc gtgggccctt cggcgtgggt cctgtggcta tcgatggggc
     1441 gatgaccggc gacggcgcgc tgacgggaac gttggccgag ccacgcgttg acctgaccgc
     1501 tggcttcgac aaggtcgacg ccgggccgct gaagatgctg cagaccaaac tgatcctgag
     1561 cttccgcaag ggcgccgacg cttccgacgg tcgggtgacg atcaccagcg gctccaacta
     1621 tggaccggcc gccgcgtcgg ggagcttctt cctcggcagc aaatccgtgc ggctgaccga
     1681 cgtcgatgtc gatgccggcg ggataaaagc gcaaggcgcg atctcgctgt cggacaatat
     1741 tccctccagc gccgacctga cgttcagcgc acggcccgga gccttcatca cctcgggcag
     1801 cgccgagggc cgggtgcggc tgacggaagg aaacggcgac gacaccgcca tactcgccgt
     1861 caccgccaga aatgcacgcc tgaccggctc gaccatcacc gttcgcagcc tcgacctgaa
     1921 cggacgcggg acgctgtcgc gcctgccgtt cacactggcg atggatgtgg gcggagacaa
     1981 tccgctgcag ttcaacggca ccggcgtcta tgcccgtcag ggcagtggtg cggatgcag
//
LOCUS       DASVTM010000326         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_456179,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000326 DASVTM010000000
VERSION     DASVTM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456179"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VF633_09000"
     CDS             complement(<1..454)
                     /locus_tag="VF633_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8471235.1"
                     /translation="MPTRRTVIATAAVAALIPAGGAFAQGRRGAAAPVATTKHGRVRG
                     FVDQGVKVFKGVRYGADTSTRRFQPAVAPEPWSEVRDALAYGAASPQKSDEPNQSEDC
                     LFLNVWTPELRDGRARPVMVYVHGGAHANGSGSSPLYDGVALCRRGDVV"
     gene            complement(473..1381)
                     /locus_tag="VF633_09005"
     CDS             complement(473..1381)
                     /locus_tag="VF633_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8471236.1"
                     /translation="MFELSQLRCFVAVAEELHFGRAAQRLNMTQPPLSRQVQLLERIL
                     GVVLLDRTSRSVRLTPAGRSFLVEAKRILRLAESAALATRRIASGDAGRVAVGFTAAS
                     GYSFLPNLVDLARTQLPNVDLTLRELVSGEQVEALLTGRIDLGLVRPPLTRPEFDKVR
                     VLTEPLVAALPSGDPRLDKETVSLSDFDAQPMVMYAPEGAGYFHGMLTAMFDEAGVSP
                     QYVQHMSQIHSILALVHARIGAAVVPEAATKLHFEGVEFRPLETTPERPVELFVAWRR
                     DNDNPSLKPLLALIQAQADHPAAGAD"
BASE COUNT          261 a          574 c          551 g          250 t
ORIGIN      
        1 cgaccacgtc gccgcgtcgg cacagggcga cgccgtcata cagtgggctc gaccctgacc
       61 cgttggcgtg cgctccgccg tggacataga ccatcaccgg acgggctcgc ccatcgcgca
      121 gctccggtgt ccagacgttg aggaacaggc agtcttcgga ctggttcggt tcatcggact
      181 tctgcggact ggccgcgccg taggctagcg cgtcgcggac ctcgctccac ggctcgggcg
      241 cgaccgcagg ctggaaccgt cgcgtcgagg tgtcggcgcc atagcgcacg cccttgaaga
      301 ccttcacgcc ctgatcgaca aacccgcgca cccgcccatg cttcgtcgtg gcgaccggcg
      361 ccgccgcccc tcttcgcccc tgcgcgaacg cacctcccgc cgggatcaat gcggcgacgg
      421 ctgcggtcgc tatgacggtc ctgcgggtgg gcatgcgagg tctccttgag gtctagtcgg
      481 cccctgcggc gggatggtcc gcctgcgcct gaatcagcgc cagcagcggc ttgagactgg
      541 ggttgtcatt gtcgcgacgc caggcgacga acagctcgac cggccgctcg ggcgtcgttt
      601 ccagaggccg gaactcgacc ccttcgaaat gcagcttggt cgccgcctcc gggaccacgg
      661 cggcgccgat gcgggcatgg accagagcca ggatcgagtg gatctgggac atgtgctgga
      721 cgtactgggg cgacaccccg gcctcgtcga acatggccgt cagcatgccg tggaagtaac
      781 ccgcgccctc tggcgcatac atgaccatcg gctgggcatc gaagtccgac agactaacgg
      841 tctccttgtc cagacgggga tcgccgctcg gaagagccgc caccagcggc tcggtcagca
      901 cgcggacctt atcgaactcg ggccgggtca agggcggtcg aaccagaccc agatcgatcc
      961 gacccgtcag caaagcctcg acctgttcgc ccgagaccag ctcgcgcaac gtcaggtcga
     1021 cgttgggcaa ctgggtccgc gccagatcga cgaggttcgg caggaagctg tagcccgagg
     1081 ccgccgtgaa cccgaccgcg acccgcccgg cgtcaccgct ggcgatccgg cgtgtggcca
     1141 gcgccgcgct ctcggccagt cgcaggatgc gcttggcctc gaccaggaac gaccgaccgg
     1201 ccggcgtcag ccgcactgac cggctggtgc ggtccagcag cacgacaccg aggatgcgct
     1261 caagcagctg gacctgacgg ctcagcggcg gctgggtcat gttgaggcgc tgcgccgccc
     1321 gcccgaagtg gagttcctcg gcgacggcga cgaagcaacg caactgactg agttcgaaca
     1381 tgttcatggc gccctgcgac ccgcgctcaa gcagcccgaa cgggcgttag cgcgcaacct
     1441 gtgtaggctg ttggtcatag gctctccggt tcgcaccgga cagtgccctg cgactatccg
     1501 ttcgccgcca gcttcggagc cgctgcgggt tgcacccgtg cgatcagcgc cgacagctcg
     1561 gccatctggg ccgcgttcag gtcgaccagc ggcgtgcgga ccggaccggc ggtgcggccg
     1621 atggcggtca tgcccg
//
LOCUS       DASVTM010000327         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_831210,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000327 DASVTM010000000
VERSION     DASVTM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831210"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1666)
                     /locus_tag="VF633_09010"
     CDS             complement(<1..>1666)
                     /locus_tag="VF633_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8471237.1"
                     /translation="EVGRKLDAIVADFIAKGPTDEEVARVATRYVSGRIQSLEQVNGK
                     ANVLAEGQLYAGDPDHYKKELAQYASVTPAQVTAAMQKWLTRPVFDLTIAPGEREAYV
                     EAAAPSGIAPAPAAEIQRVARDPMPTIGQVPDLDFPAVERATLSNGVKVIYAHVDTVP
                     VTRVAVEFDAGFAADRADRLGAHSLMLDLLDDGTTTRDANQLAEEQERLGASIQVGAS
                     MDRTSANLTAVTANLTPSLILMADVVRNPAFAPSEVERLRAQRLAALSSEATQPGAIA
                     ARALPPLLYGADSPYGRSFTGTGDASTITALTRADLVQEHDEWVRPDNATIFVVSDLP
                     LSQVTPQLEAAFGDWRAPSIAKGTKAFSDAVPAPTNRIVLIDRPQSPQSLIYGGAVLP
                     LSGTDDLLTLTSANSILGSDFLSRINADLRETKGWSYGVRGNVNPLEHRVPYIVNAPV
                     QANRTGESVAALIAQYDRFLKADGVTPLELERTINGNTRQLAGGYETSAQILGALRSN
                     ALYGRPDDYQETIASRTRALTAAQMDDAARQVIRPDQFVWVVVGDAS"
BASE COUNT          260 a          515 c          605 g          286 t
ORIGIN      
        1 gaggcgtcgc cgaccaccac ccagacgaac tgatcgggcc ggatgacctg acgcgcggcg
       61 tcgtccatct gggctgctgt cagggcgcga gtgcggctgg cgatcgtctc ctgatagtcg
      121 tccggacggc catacagggc gttggaccga agggcgccga gtatctgcgc cgaggtttcg
      181 taaccgccgg ccagctgacg ggtgttgccg ttgatggtcc gctccagctc cagcggcgtg
      241 acgccgtctg ccttcaggaa gcggtcgtac tgggcgatca gggccgcgac ggactcaccc
      301 gtgcgattgg cctgcaccgg cgcgttgacg atgtagggaa cccggtgctc cagcgggttg
      361 acgttgccgc gcacgccata cgaccagccc ttggtctcgc gcagatcggc gttgatccgc
      421 gacaggaagt ccgagcccag gatcgagttg gcggaggtca acgtcagcag atcgtctgtg
      481 cccgacaacg gcagcacggc gccgccgtag atcagcgact gcggcgattg cggacgatcg
      541 atcaggacga tccggttggt cggcgccgga accgcgtcgc tgaacgcctt cgttcccttg
      601 gcgatcgagg gggcgcgcca gtcgccaaac gcggcttcga gttgcggcgt gacttgcgac
      661 agcggcaggt cggagacgac gaagatcgtc gcgttgtccg gccgcaccca ttcatcgtgt
      721 tcctgaacca ggtcggctcg cgtcagggcg gtgatcgtgg acgcatcgcc agtaccggtg
      781 aaggaccggc cataggggct gtcggcgccg taaagcagcg gcggcagggc gcgggccgca
      841 atcgcgcctg gctgggtcgc ctcgctggac agggcagcca gtcgttgcgc gcgcagacgc
      901 tcgacttcgg agggtgcgaa ggccgggttg cgaaccacgt ccgccatcag gatcagcgac
      961 ggcgtcaggt tggcggtgac ggccgtgagg ttggcggagg ttcggtccat cgatgcgccg
     1021 acctggatcg aggcgccgag acgctcctgt tcctcggcca gttggttggc gtcgcgggtc
     1081 gtggtgccgt cgtccagcag atccagcatc aggctgtgag cgcccagacg gtctgcacgg
     1141 tcggcggcga aaccggcgtc gaactcaacc gccacgcggg tgaccggcac ggtatcgacg
     1201 tgtgcgtaga tcaccttcac gccgttcgac aaagttgcgc gttcgacggc ggggaagtcg
     1261 aggtcgggga cctggccgat ggtgggcatc ggatcgcggg cgacgcgttg gatttcggcg
     1321 gccggtgcgg gcgcgatgcc cgacggagcg gcggcttcga cataggcttc acgctctccg
     1381 ggagcgatgg tcaggtcgaa caccggacgg gtcagccatt tctgcatcgc cgccgtgacc
     1441 tgcgccggag tgaccgacgc gtactgggcc agctccttct tgtagtggtc cggatcgccc
     1501 gcatagagct gaccctcggc cagcacattg gctttgccgt tgacctgttc gaggctctgg
     1561 atacggcccg agacataccg agtggcgacg cgggcgacct cttcgtcagt ggggcccttg
     1621 gcgatgaagt cggcgacgat ggcgtccagc ttgcgaccga cttcag
//
LOCUS       DASVTM010000328         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_299874,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000328 DASVTM010000000
VERSION     DASVTM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299874"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VF633_09015"
     CDS             complement(<1..1166)
                     /locus_tag="VF633_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8471238.1"
                     /translation="MRGSERGGARSGRRADPETKALAGASADKPEEPNTFAALADLVR
                     LVGRSGAPQLRLRLVCAIALTLAGKGLGVLAPLVLGAAVNHLSRGQGAGVSVGLGFAA
                     FAAGWALVRFLSSVTPQISDVIFAPVRAAAQRKTAGETFAHALNLSLDFHQTKRSGSL
                     SRTVDRGSRAVDFLLRILAFNLVPTALELVLAAGVLGGKYDWRFAAVAVVVVVIYASL
                     TFGISNWRIEHRRTMNTADSEAAGLAVDALLNYETVKSFGAEARAAEGYDRALGDYNV
                     AALKANTSLALLNAVQGLIMNLGLGVMAVMAGFEAAAGRMGPGDVTAAVLILISLYAP
                     LNILGFAYREIRQSFIDMEEMLKITRQSPQVADAPNAPDLPKPDDTRGGSITFEG"
     gene            1434..1793
                     /locus_tag="VF633_09020"
     CDS             1434..1793
                     /locus_tag="VF633_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX8471239.1"
                     /translation="MSKLQESREEAIKRLNDSASALEHRNAPPTSEHLAGLAVTSQAY
                     KIIAELIGGVFVGLGLGAAVDWFFKTTPWGLIGGVLAGFALSIWMARRTADRLMAQAK
                     ASGIVPQAVPFDDAEED"
     gene            1806..2609
                     /locus_tag="VF633_09025"
     CDS             1806..2609
                     /locus_tag="VF633_09025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX8471240.1"
                     /translation="MADPIHQFQIVPLPGLDFGEVTLPLIGTVHLAVTNSHIAMTLAF
                     AAVVLFLSIVTANAKVVPGRTQAAGEAVFGLIDNLTHSIIGHEGKRYFPFVFTLFMLI
                     LGMNLLGLLLTFTVTSQLAITATFAVLTILLVIGVGFARNGLGFFLLFWPTSAPLAIR
                     PVVGLIEFLSFLLRPVTLALRLFGNMLGGHIALKIFAGFVVSMGGLFLAAGEFNALGL
                     LGLPVAALSLTMVVGLTALEFLVAGLQAFVFAVLACIYLNDVVNLEHAH"
     gene            2662..2886
                     /locus_tag="VF633_09030"
     CDS             2662..2886
                     /locus_tag="VF633_09030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX8471241.1"
                     /translation="MDAASFKYFGIGLATLGMLGSAIGVGNIFAAFLGGALRNPSAAA
                     SQVGNLFVGAALVEALGILAFVLGILAWLG"
     gene            2958..3563
                     /locus_tag="VF633_09035"
     CDS             2958..3563
                     /locus_tag="VF633_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471242.1"
                     /translation="MAAHSSAPPPVLPVEDAVGHAVANGHAAGPNTAGEVAEHGTGGL
                     PQFETQHWFGQIAYLLILFFILLFLISKVFAPRLRRVMAERADTIATAVETARSVQAE
                     AASQAALAKAEVTQARAESRSLAAAAKARVTEEAARRAAEEEAVVNARIAEAETAIGK
                     TRDAAMTQVSSIAADTAAAIVEHLTGKAATPVELAAVKGTV"
     gene            3565..4080
                     /locus_tag="VF633_09040"
     CDS             3565..4080
                     /locus_tag="VF633_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX8471243.1"
                     /translation="MPVFLSGEFWSLSNPELWVGVGLLLFFGVLIMAGVPKLAGTQLD
                     AAASKIQTELDEAARLRAEAEALLAQIRAEKIEADAQAAAMLKAAEDDARVMEAEAKA
                     KLEETLVRRQALAERRIAQAEAQATADVKAAAADQAARVAEQILTARVSGQKSDPLLD
                     AAIAQIGSRLN"
BASE COUNT          722 a         1459 c         1293 g          764 t
ORIGIN      
        1 ccttcgaagg tgatcgaccc gccgcgcgtg tcgtccggct tgggcaggtc cggcgcattg
       61 ggcgcatcgg cgacctgggg agattggcgc gtgattttca gcatctcctc catgtcgatg
      121 aaggattgcc gtatttcgcg ataggcgaag ccgaggatgt tcaagggcgc atagagcgag
      181 atcagtatca gcacggccgc tgtgacgtcg ccggggccca tccgccccgc cgccgcctcg
      241 aatccggcca tcacggccat gacgccgagg cccaggttca tgatcaggcc ctgtacggca
      301 ttcagcagcg ccagcgaggt gttggccttc agcgccgcga cattgtagtc gcccagcgcc
      361 cgatcgtagc cctcggcggc gcgtgcctcc gccccgaacg acttgaccgt ctcgtagttg
      421 agcagggcat cgacggcgag accagcggct tccgaatcgg ctgtgttcat cgtccggcga
      481 tgctcgatcc gccagttgga aatgccgaag gtcaggctgg cgtagatgac aaccacgacc
      541 acggcgacgg cggcgaatcg ccagtcgtac ttgccgccca gaaccccggc cgccagcacc
      601 agttcaagcg ctgtcggcac gaggttgaaa gcaaggattc gcagcaagaa atcgacggcc
      661 cgcgaacccc ggtccaccgt acgggacagg gagccggacc gtttggtctg gtggaaatcc
      721 agcgacagat tcagcgcgtg ggcgaaagtt tcccccgcag ttttacgctg ggccgccgcg
      781 cggacgggtg cgaagatcac gtcggatatc tggggcgtga ccgacgacag gaaccgcacc
      841 agcgcccagc ctgcggcaaa cgcagcgaag cccagcccga ccgagacgcc cgccccctgc
      901 ccgcgtgaca gatgattgac cgcagcgccc agcaccagcg gcgccagcac gccaagccct
      961 ttgccggcca gcgtcagggc gatcgcgcag accagccgca gacgcagttg gggcgcgccg
     1021 gaacgtccca ccaaccgcac cagatctgcc agtgcagcaa aggtattggg ctcctcaggc
     1081 ttgtccgccg aagctcccgc cagcgccttg gtttccggat cggcgcgacg tccgctacgg
     1141 gcgccgccgc gttcactgcc tctcatggtc atggactgca tatggattgt gcggcgccgg
     1201 gagggaagag cggaggcgta agcgcgcaca gccatcaggc cttccgacgc cacctgctac
     1261 cgacgcagag tggcggcgct tcgtcacacc tgtgaatggc cgaaaaaact ttagccctat
     1321 cagacagtta gataggctgt aacacgcctt gaccaaactg cccctcgcct ataggttccg
     1381 cgccgaattc agacccgctt cggggttctc ggagcgctga gtaaacacaa cccatgtcca
     1441 agcttcagga atcgcgcgaa gaggcgatca aacgcctcaa cgacagtgct tccgcgctgg
     1501 aacacaggaa tgcgccgccg acctccgagc atctcgcggg gctggcggtg acgagccagg
     1561 cctacaagat catcgccgag ctgatcggtg gtgttttcgt gggattaggt ttgggggcgg
     1621 ccgtggactg gtttttcaaa accactccgt ggggtttgat cggcggagtc ctcgcgggct
     1681 tcgccttgtc gatttggatg gcgcgccgga cagccgacag gctgatggcg caggcgaaag
     1741 cgagcgggat cgtgcctcag gccgtgccgt tcgacgacgc cgaagaagac tagggaagcg
     1801 actgcgtggc cgatccgatt catcaattcc agatcgtccc gcttccgggt ctcgattttg
     1861 gcgaagtcac cctgccgctc atcggcacgg tgcacctcgc cgtgactaac tcccacatcg
     1921 ccatgacact ggccttcgcg gccgtggtgc tgttcctgtc gatcgtcacg gccaacgcca
     1981 aggtcgttcc gggtcgcaca caggccgcag gcgaggccgt ctttggtttg atcgacaacc
     2041 tgacccattc catcatcggc catgagggca agcggtattt cccgttcgtc ttcaccctgt
     2101 tcatgctgat cctcggcatg aacctgctgg gcctgctgtt gaccttcacg gtcacctcgc
     2161 aacttgccat caccgctacg ttcgccgtgc tgaccatcct gctggtcatc ggcgtcggct
     2221 tcgctcgcaa cggtctgggc ttcttcctgc tgttctggcc gaccagcgcg ccgctggcga
     2281 tccgcccggt ggtgggcctg atcgagttcc tgtctttcct gctgcgcccc gtcaccctcg
     2341 ccctacgttt gttcggcaac atgctgggtg gtcacatcgc gctgaagata ttcgcgggct
     2401 tcgtggtctc gatgggcggg ctgttcctcg cggccggtga gttcaacgcg ctcggccttc
     2461 tgggtctgcc cgtcgccgcc ctgtcgctga ccatggtcgt cggcctgacc gctctcgagt
     2521 tcctcgtggc cggcctgcaa gctttcgttt tcgccgttct ggcctgcatc tacctcaatg
     2581 atgtggtcaa cctggaacat gcccactaag ggccgctcca gtttcctcta acctaccaac
     2641 ctgactgaat aggatttcat catggacgcc gcctccttca aatacttcgg catcggcctg
     2701 gccacgctcg gcatgctcgg ctccgctatc ggcgtgggca acatcttcgc cgcattcctg
     2761 ggcggcgccc tgcgcaaccc ttcggccgct gcttcgcaag tcggcaacct tttcgtcggc
     2821 gccgctctgg tcgaagccct gggcatcctg gccttcgttc tgggcatcct ggcctggttg
     2881 ggctgatacc gacgactatc ctgacgacgg cgtcctgacc ggacgccgtc gtttcgcttt
     2941 tgcttcacgg actagatatg gccgctcatt cctccgcccc gccgcccgtt ctgccggtgg
     3001 aagacgccgt cggacacgcg gtcgcgaacg gccacgccgc aggtccgaac accgcaggtg
     3061 aagtcgccga gcacggcacg ggcggcctgc cgcaattcga gacccagcac tggttcgggc
     3121 agatcgccta cctgctgatc ctcttcttca tcctgctatt cctgatttcc aaggtcttcg
     3181 caccgcgtct gcgtcgcgtc atggccgagc gtgccgacac gatcgccacc gctgtcgaga
     3241 ccgcccgcag cgtgcaggct gaagccgcct cacaagccgc actggccaag gccgaagtca
     3301 ctcaggcccg cgccgaatcg cgcagcttgg ccgccgccgc caaggctcgc gtcaccgaag
     3361 aggccgcccg tcgcgccgcc gaggaagaag ccgtcgtcaa cgctcgcatc gccgaggccg
     3421 agaccgccat cggcaagacc cgcgacgccg ccatgaccca ggtctcctcc atcgccgccg
     3481 acacggcggc cgctatcgtc gaacacctga ccggcaaggc cgcgacgccc gtcgagctag
     3541 ccgccgtcaa gggaaccgtc tgacatgccc gtttttcttt ccggtgagtt ctggtcactc
     3601 tccaaccccg aactgtgggt cggcgtcggc ctgctgctgt tcttcggtgt tctgatcatg
     3661 gctggcgtgc ccaagctcgc cggcacacaa ctcgacgctg cagcctcgaa gatccagacc
     3721 gagctagacg aggccgcacg cctgcgcgcc gaagccgagg ccttgctggc ccagatccgt
     3781 gccgaaaaga tcgaggctga cgcccaggcc gctgccatgc tgaaggccgc cgaggacgac
     3841 gcccgtgtga tggaagcgga ggccaaggcc aagctcgagg aaacgcttgt ccgccgtcag
     3901 gctttggccg agcgtcgcat cgcccaggcc gaggcccagg cgactgcaga cgtgaaggcc
     3961 gccgccgccg accaggctgc gcgcgtcgcc gaacagatac tgaccgcccg cgtttcgggg
     4021 cagaagagcg atccgctgct cgacgccgcc atcgcccaga tcggcagccg cctcaactag
     4081 agacggcttc aaggcaaacg acatcaggcc cgccggtcac cggcgggcct ttttcgtggt
     4141 cagtgcgcag gaatggtgcc ggccgccagc gcccgggccg cacgcttctc gcgggtgacc
     4201 agttcgccat agacggtccc cagcacgata ccgaacac
//
LOCUS       DASVTM010000329         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_669268,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000329 DASVTM010000000
VERSION     DASVTM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669268"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2531
                     /locus_tag="VF633_09045"
     CDS             <1..2531
                     /locus_tag="VF633_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471244.1"
                     /translation="IAESLGRLPGLTTQRLDGRSQALSIRGLGPDFTTALLNGREQVT
                     TGDNRGVEFDQYPSELLSGVTVYKTPDAALIGQGLAGTVDLQTVRPLAYGRRAIAANA
                     RYEMNDIGALNSGTTDHGNRYSISYIDQFLDGTLGVALGYAHMESPYQSERFNSWGYP
                     TLADGNLVEGGLKPYVMSSELKRDGYMGVLEWKPNDRFSSTIDAFYSKFENSQVLRGI
                     EFPLQWGGSAGDCTVNVQSAICRPGPALRPGYTVQDGLVTAGTFDNVKGVVRNDFNGR
                     DSEITSVGWNTKFQATDDWSFALDLNYSKVERTDTIVEGYSGTGRNIAGALDTLGFTV
                     DGDGVTHLSSTINYADPNLIRLTSPQGWGSDIIPGGQDGYLNTPTITDEIKAIRLSGR
                     RELHQGPFSSIDFGFNYSEREKSFVNDQYFLGVPGGATVVVPAAFLLAPTELGYLGIP
                     AVLSYDTIGLINSGFYNLVRNPNADVVSGNWEVTEKVSTTFIRANIDHNLFGMPLTGN
                     VGFQAVYTDQSSNGFSASGTGATAVSLPVTGGAQYLEILPSSNFILEVGNDMFARFAV
                     ARTLARPRMDDMRASRNFSFDVSKNIPAAQPDLNSPWGGGGGNPELKPYIADVVDLSF
                     EKYFANRRGYVSVAAFYKNLETFVYTMNQIFDFTGYPTGGVTPVINQGVVSAAANGSG
                     GYIEGVEFALSLPAEIIHPMLDGFGAQFSISQTSSEIQPDPTQAPTAVPGLSETVANL
                     TAYYEKYGFQARVSGRYRSDYLGEVAGFGNGRTLRSVAAETVVDAQIGYQFQSGPLEN
                     LSLLAQVNNLTDEPFKTFQNGDERQTIDYQSYGRTFLFGVSYRY"
BASE COUNT          481 a          914 c          749 g          475 t
ORIGIN      
        1 ccatcgccga atcgcttggc cgtctgccgg gcctgacgac gcagcgcctc gacggccgct
       61 ctcaggccct ctcgatccgc ggcctcggcc cagacttcac gactgctctg ctgaacggtc
      121 gcgagcaggt cacgaccggc gacaaccgcg gcgtcgagtt cgaccagtat ccatccgaat
      181 tgctgagcgg cgtgacggtc tacaagaccc cggacgccgc cctgatcggt caaggcctgg
      241 ccggcaccgt cgatctccag accgtgcgac ccctggccta tggccgccgc gccatcgccg
      301 ccaacgcccg ctatgagatg aacgacatcg gcgcgctgaa ctcgggtacg accgaccacg
      361 gcaaccgcta ctcgatctcg tacatcgacc agttcctgga cggtacgctg ggcgtcgccc
      421 tcggctacgc tcacatggag agcccgtatc agtcggagcg cttcaacagc tggggctatc
      481 cgacccttgc ggacggcaat ctggttgagg gcggcctgaa gccctacgtc atgtccagcg
      541 agctgaagcg cgacggctac atgggcgtgc tggagtggaa gccaaacgac cgcttctcct
      601 cgaccatcga cgccttctat tcgaagttcg agaactcgca ggtcctgcgc ggcatcgagt
      661 tcccgctgca gtggggtggc tcggccggcg attgcaccgt caacgtccag agcgcgatct
      721 gccgtcccgg tcccgcgctt cgccccggct acaccgttca ggacggtctg gtcaccgccg
      781 gcacctttga caacgtaaag ggcgtggtcc gcaacgactt caacggtcgt gacagcgaga
      841 tcacctcggt cggctggaac acgaagttcc aggccacgga cgattggtcc ttcgctctcg
      901 acctcaacta ttcgaaggtt gaacgcacgg acaccatcgt tgagggctac tccggtaccg
      961 gccgcaacat cgcaggcgcg ctcgacacct tgggcttcac cgtcgatggc gatggggtca
     1021 cccacctgtc cagcacgatc aactatgcgg atccgaacct gatccgcctg accagcccgc
     1081 aaggctgggg cagcgatatc atcccgggcg gccaggacgg gtatctgaac acccccacca
     1141 tcaccgacga aatcaaggcc attcgcctgt cgggtcgtcg tgaactgcac cagggcccgt
     1201 tcagctcgat cgacttcggc ttcaactact ccgaacgtga gaagagcttc gtcaacgacc
     1261 agtatttcct tggcgttccc ggcggcgcga ccgtcgtggt gccggctgcg ttcctgctgg
     1321 ctccgaccga actgggctac ctcggcattc cggcggtgct cagctacgac acgatcggcc
     1381 tgatcaacag cggcttctac aacctcgttc gcaacccgaa cgcggacgtg gtctccggca
     1441 actgggaagt gacggaaaag gtctcgacga ccttcatccg cgccaacatc gaccacaacc
     1501 tgttcggcat gcctctgacc ggcaacgtcg gcttccaggc ggtctacacg gatcagagct
     1561 ccaacggctt ctcggccagc ggcaccggcg caaccgccgt gtccctgccc gtgaccggcg
     1621 gcgcgcaata tctggaaatc ctgccaagct cgaacttcat ccttgaagtc ggcaacgaca
     1681 tgttcgcccg cttcgccgtc gcccgcaccc tcgcgcggcc gcggatggac gacatgcgcg
     1741 cctcgcggaa cttcagcttc gacgtctcga agaacattcc ggccgctcaa cccgacctga
     1801 actccccttg gggcggcggc ggcggcaacc cggaactgaa gccttatatc gccgacgtgg
     1861 tggacctctc gtttgagaag tatttcgcca accgtcgcgg ctatgtctcg gtcgcagcct
     1921 tctacaagaa cctggaaaca ttcgtttata cgatgaacca gatcttcgac ttcaccggct
     1981 atccgaccgg cggcgtcaca ccggtgatca accagggcgt ggtttcggct gcggccaacg
     2041 gctccggcgg ctatatcgag ggtgtcgagt tcgccctgtc gttgccagct gaaatcatcc
     2101 atcccatgct cgatggcttc ggcgcccagt tcagcatctc gcaaacgtcc agcgagattc
     2161 agcccgaccc gacccaggcc ccaaccgccg tccccggtct gtcggagacc gtcgccaacc
     2221 tgaccgccta ctacgaaaaa tacggcttcc aggcgcgcgt ttcgggccgc tatcgttcgg
     2281 attacctcgg cgaggtcgcc ggtttcggca acggtcgcac cctgcgttca gtcgccgccg
     2341 agaccgttgt cgacgcccag atcggctatc agttccagtc gggtccgctc gagaacctgt
     2401 cgctgctggc ccaggtcaac aacctgaccg acgagccgtt caagaccttc cagaacggcg
     2461 atgagcgtca gaccatcgac taccagagct atggccggac cttcctgttc ggcgtcagct
     2521 accgctacta gcggccgctg agagagaacc ggtgtcgggc gtttcctccc tgactcgaca
     2581 ctgaaacttg cgccgctcgt ccatccgggc gagcggcgt
//
LOCUS       DASVTM010000330         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_831274,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000330 DASVTM010000000
VERSION     DASVTM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831274"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VF633_09050"
     CDS             <1..622
                     /locus_tag="VF633_09050"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8471245.1"
                     /translation="ALVLKAELEGTGRYRVMLTRQNDSYVDLYRRVRIARTADADLFI
                     SLHADAGTDPATRGASVYTLSEQGASRAVREVTRGDNWHRDLHLPGRDPSVDRILLDM
                     TQRATQNRSAQFARVLLTHMEAADQPLLRRSHRDAGLAVLLAPDVPAVLLEMGFITNP
                     DDERALGDPVRRRRLMRAVSEGIDRYFREPSAPLQMASTGMGAGGL"
     gene            685..1383
                     /locus_tag="VF633_09055"
     CDS             685..1383
                     /locus_tag="VF633_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX8471246.1"
                     /translation="MRLRVVDLETTGGDRTSEIIEVGFVDVVSDEEGWKALPPVSKLF
                     RPRGEISFHAMAVHHLTPEHFCDGDPHCDEYKLREMFAEPADVMVAHSARFERAFIAD
                     TATGGLPWICTVRSSKGAWPEAPGHSNQVLRYWRGFRLDPTLADPAHRAGPDAWVTAH
                     ILMDLLKEHSIEKLLEWSNAPRDIATVPFGKHRRKAWSEVPEDYLRWMASQTDMDVDA
                     VTAARNELDKRKAA"
BASE COUNT          262 a          497 c          503 g          271 t
ORIGIN      
        1 cgccttggtg ttgaaggccg agttggaggg cacgggtcga tatcgcgtga tgctgacccg
       61 tcagaacgat agctacgtcg atctctaccg ccgggtacgg atcgcccgga cggccgacgc
      121 cgatctgttc atctcgctgc acgccgacgc aggcaccgac cctgccacgc gcggggcctc
      181 ggtctatacc ctgtccgaac agggggcgtc gcgggccgtg cgcgaggtca cccggggcga
      241 caactggcac cgcgacctgc acctgccggg tcgcgatcca tcggtggacc gcatcctgct
      301 cgacatgacc cagcgcgcga cccagaaccg ctcggcccag ttcgcccgcg tccttctgac
      361 ccacatggaa gccgcagatc aaccattgct tcgtcgcagc catcgcgacg ccggtctggc
      421 tgtactgctg gcgccggacg ttcccgccgt gctgctggag atgggcttca tcaccaaccc
      481 cgacgacgaa cgcgccctcg gtgacccggt ccgtcgtcgc cgtctgatgc gcgccgtctc
      541 cgaaggcatc gaccgctatt tccgcgagcc gagtgctccg ctgcagatgg cttcgacggg
      601 catgggcgca ggcggactgt aatccacgac cggacctgac gcagcgacgc tggcaagacc
      661 ggcttagcgg actatatcgg cacaatgcgt ctgagagtcg tcgatctgga aaccacgggc
      721 ggggaccgca cctccgaaat catcgaggtc ggcttcgtcg atgtcgtgtc ggatgaggaa
      781 ggctggaaag ccctgccgcc ggtgtcgaaa ctgttccggc cgcgtggcga aatctcgttc
      841 cacgccatgg ccgtgcacca cctgacgccc gagcatttct gcgacggcga tccgcactgc
      901 gacgaataca agctgcgtga gatgttcgcc gaaccggcgg acgtgatggt cgcccacagc
      961 gcgcgattcg agcgggcctt catcgccgat accgccactg gcggcttgcc gtggatatgc
     1021 actgtgcgct cttcgaaggg cgcgtggcct gaggctcccg gccattccaa tcaggtgctg
     1081 cgttactggc gcggcttcag gctggatccg acgctggccg accccgcgca ccgggcaggg
     1141 ccggacgcct gggtcaccgc ccacatccta atggacctgc tcaaggaaca cagcatcgag
     1201 aagctgttgg agtggtccaa cgcgcctcgc gacatcgcta ccgtgccctt cggcaaacac
     1261 cgtcggaaag cgtggtcgga agttccagaa gactatctca gatggatggc atcgcagacc
     1321 gatatggatg tcgatgcggt cacagcggcg aggaacgaac tcgacaagcg aaaagcggcc
     1381 taggcttggc tggcaacgcg gtctaaaccc gctgcttggg gtgtttccgt cggagtttgc
     1441 ggtgaaggtt gcggagcgct ggttcgtgtg ggcgggcgtt ggcctgctgg gcgctattgc
     1501 gctggccggg ctggtggtcg ctgtctatgc agc
//
LOCUS       DASVTM010000331         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_778020,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000331 DASVTM010000000
VERSION     DASVTM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778020"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF633_09060"
     CDS             <1..342
                     /locus_tag="VF633_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX8471247.1"
                     /translation="TQISLAAGERVTHAPATGTICDALMTDRPGELTFPINRRLTGVL
                     TVTDAEVAEAVRYAFRTLKLVVEPGGAVSLAALLTGKISTDGKTTAIVLSGGNVDPAL
                     FSAIIEDRFEG"
     gene            431..904
                     /locus_tag="VF633_09065"
     CDS             431..904
                     /locus_tag="VF633_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8471248.1"
                     /translation="MTKATDLEALIGTEVGVSKWIEVDQKRIDAFADATEDWQFIHVD
                     PEAAAKTPFGGTIAHGFLTLSLMSAMTYDANQPLEGVVMGVNYGFDKLRFINPVRSGS
                     KIRGRFKLLSAENKGTDNGVTRWLIKHEVTVEIEGLAKPALIAEWLGMQMIADAG"
     gene            1054..1467
                     /locus_tag="VF633_09070"
     CDS             1054..1467
                     /locus_tag="VF633_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471249.1"
                     /translation="MAVEILVAEDNEDVRDFLVAALSGAGFRVVEAADGAAVLTRLGY
                     APPGLILLDLHMPLVDGMEVLRRLRSVPAWEKTLVLILTASGLTDDLVEARRLGARGY
                     LVKPIRPADLVAKVKRILEDPELIWLDDITELRAR"
     gene            complement(1484..>2509)
                     /locus_tag="VF633_09075"
     CDS             complement(1484..>2509)
                     /locus_tag="VF633_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8471250.1"
                     /translation="NYVGRIDNASRALLSTVNDILDFSKLEAGQVAIRPRPTDIAELC
                     RQTLELFSPQAGAKDLALHFEVAADRRWIQVDPDRIRQILLNLVGNAVKFTASGAVTL
                     ALSWDQDSERLHLVVKDTGEGVPADKLEKLFKRFSQVDGSLTRNHGGTGLGLAICKGL
                     AEAMGGRIGADSVIGTGSRFWFDIPAPRAHGVTIADDGDGAPRALPALTGKRVLVADD
                     HPANRELARLFLSGVGAQVVDANDGVAAVEVAHAQRFDVILMDMRMPRLDGAAALGRI
                     RGGGGVNAMTPILAYTADAGADEDRRLVALGFSGVVAKPVSSAALLDAIADAVLGPHS
                     AALGRLA"
BASE COUNT          437 a          828 c          825 g          419 t
ORIGIN      
        1 acccagatct cgctggcagc gggcgagcgg gtgacccacg ctcccgcaac tggcacgatc
       61 tgcgacgccc tcatgaccga ccggccgggc gagctgacct tcccgatcaa ccgacggctg
      121 acgggcgtgc tgaccgtcac cgacgccgag gtggccgagg cggtccgcta tgctttccgc
      181 accctgaagc tggtggtcga gccgggcggc gcggtatcgc tggccgcgct gctgaccggc
      241 aagatttcta ccgacggcaa gaccaccgcc atcgtcctgt caggcggcaa cgtcgatccg
      301 gccctcttct cggccattat cgaggatcgc ttcgagggtt gagatcgtgc tggcgtaacc
      361 ttgggtaagg cggatagcgt tccctcccaa ggcccgcagc tgacgggtca ggacgtaaag
      421 ggaaacgcac atgaccaagg ccacggatct cgaagccctc atcggcacgg aagtcggcgt
      481 ctcgaaatgg atcgaggtcg atcaaaagcg catcgacgcc ttcgccgacg ccactgagga
      541 ctggcagttc atccacgtcg atcccgaagc cgcagcgaag accccgttcg gcgggaccat
      601 cgctcacggc ttcctgaccc tcagcctgat gtcggccatg acctatgacg ccaatcagcc
      661 gctcgagggc gtggtgatgg gcgtcaacta cggcttcgac aaactgcgct tcatcaaccc
      721 cgtgcggtcc ggctcgaaga tccggggccg gttcaaactg ctgtcggccg agaacaaggg
      781 caccgacaac ggcgtcacac gctggctgat caagcacgag gtcaccgtcg agatcgaggg
      841 cctcgccaaa cccgccctga tcgccgaatg gctggggatg cagatgatcg ccgacgcggg
      901 ctgatcgtgg ggggatatcc ggatctagcg acggtgtcgt gatcgacttc gacgatccac
      961 ccgggagtaa gtgtctcttg cgactgctgt tcagcgaccg gaaatcacct tagcgttggc
     1021 aaccgagatc gtgtcggtca cgggagtagg ctgttggctg tcgagatatt ggtcgccgag
     1081 gacaacgagg atgtgcgcga tttcctggtc gccgccctgt ccggggccgg ttttcgggtg
     1141 gtcgaggccg cggacggcgc cgcggtcctg acgcggctgg gctatgcgcc gccgggactg
     1201 atccttctgg acctgcatat gccgctggtt gacgggatgg aggttctgcg ccgcctgcgc
     1261 tccgtgcctg cgtgggagaa gacgctggtg ctgatcctga cagcgtcggg cctgaccgac
     1321 gatctggtcg aggcacgccg gctgggcgcc cgtggttatc tggtcaagcc gatccggccg
     1381 gcggacctgg tggccaaggt caagcgcatc ctcgaagacc ctgagctgat ctggctcgac
     1441 gacatcaccg aacttcgcgc ccgctagcag gcgaggctcg acctcaagcc agccggccca
     1501 gagcggcgct gtgcggcccc agaacggcat ccgcgatggc atcaagcagg gccgccgagg
     1561 agaccggctt ggcgaccacg cccgagaacc ccaacgcaac caggcggcga tcctcgtccg
     1621 cgcctgcatc ggcggtgtag gcgaggatcg gcgtcatcgc attgaccccg ccgccgcccc
     1681 tgatccgccc caacgctgcg gccccgtcga ggcgtggcat ccgcatgtcc atcaggatca
     1741 cgtcgaatcg ctgggcatgc gctacctcga ccgcagccac gccgtcgttc gcatcgacga
     1801 cctgggctcc gacgccggac agaaacagac gcgcaagttc ccggttggcc ggatgatcgt
     1861 cggccaccag aacgcgcttg ccggtcaggg cgggcagcgc ccggggcgcg ccatcgccgt
     1921 catccgctat cgtcacgccg tgggcgcggg gcgcgggaat gtcgaaccag aagcggctgc
     1981 cggtcccgat gacactgtcg gccccgatcc ggccgcccat ggcctcggcc aatcccttgc
     2041 agatcgccag acccaggccg gtaccgccgt ggttgcgggt caatgagccg tcaacctgcg
     2101 agaaccgctt gaacagtttt tccagcttgt cggcgggcac gccctcgccg gtgtccttga
     2161 ccacaaggtg gagtcgctcg ctgtcctgat cccagctgag cgcaagtgtc acggcgccag
     2221 aggccgtgaa cttgaccgca ttgccgacca ggttgagcag gatttggcga atacgatccg
     2281 gatcgacctg gatccagcgc cggtcggccg ccacctcgaa atgcagggcc agatccttgg
     2341 cgcctgcttg cggcgagaac agctccagcg tctgtcggca cagttcggcg atgtcggtcg
     2401 ggcgtgggcg gatcgcgacc tgacctgcct ccagcttgga gaagtcgagg atgtcattga
     2461 cggtcgacag caaggcccgg ctcgcgttgt cgatgcggcc aacgtaatt
//
LOCUS       DASVTM010000332         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_32481, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000332 DASVTM010000000
VERSION     DASVTM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32481"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VF633_09080"
     CDS             complement(<1..332)
                     /locus_tag="VF633_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX8471251.1"
                     /translation="MPSPKRLIHLHWPLLLALACAVAVGLLTPASWRWPTRMASGWDA
                     GVALFLLITMIKVVRIQSVGQIRKRAAELDDAGSAVLPLSVVAALASVVIVMGETATA
                     ASNEAVSSA"
     gene            511..1392
                     /locus_tag="VF633_09085"
     CDS             511..1392
                     /locus_tag="VF633_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8471252.1"
                     /translation="MLSIKNLVHVYGNGTRALDDVSLEIPTGMFGLLGPNGAGKSTLM
                     RSLATLQTPTSGTIDFDGINVIKDPEKLRRTLGYLPQDFGVYPRVSAYDMLDHMAVLK
                     GIASGKDRKATVESLLQQVNLWPVRNKALAGFSGGMRQRFGIAQALIGNPKLIIVDEP
                     TAGLDPEERNRFLNLLAEIGEQVVIILSTHIVEDVADLCPRMAVLAGGKIQLQGAPQE
                     LLKQMEGRVWKKTIAKEALEDHRTRHDVISTRLFAGQTVIHVLADRRPGEGFEPVSGG
                     LEDVYFSTLTASRKVAA"
     gene            1402..>3750
                     /locus_tag="VF633_09090"
     CDS             1402..>3750
                     /locus_tag="VF633_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX8471253.1"
                     /translation="MFLKVASFEFRYQLRQPAFWVIAIIFTLLGFGIVAAPGNVSIGG
                     GGNVYKNAPYILSILNMVMATFFMLGSTVIVANVVVRDVQSGFGPMIQSTRLSKFDYL
                     FGRFTGAFAATALCFLSVSLGVLAGTLMPWIDRELVGPFRPGDYVYAYLIMGLPGVFL
                     SSALFFVLATLTRSMMATYVGVVALLIVYLTATGTLGSRPEFEVAMAWAEPFGSGAYG
                     LATKYWTAVEKNTLNAPLVGVLLWNRVIWTAVAAAVLASAYWLYQPALRGKKAKTQDK
                     LRAIAEAAPAPAPLGILPSPTYGLASAWTQFAKRTAFEMSMVFKSPALAILLVLGLGF
                     SLVTLLFAGEIYGTPTLLVTRMVVQTLMGSFGLISMIVAIYYSGELVWRDRERKAQEI
                     VDSSSAPDWTFLIPKTMALILVLFSILLVGILSGVVIQTFKGYTNYEFGKYLAWYLVP
                     ETLDYAVLAVLAIFIQSLSPNKFVGWAIMVLYLISTIVASGLGFDHVLYRYGSGIGGP
                     LSDMNGSGHFRGFSNWVDAYWSAAAVILLTLAYGLWRRGTETRFMPRLKRLPRRLKGT
                     AGVIGGVAVAAFIGLGVFIFVNTNVWNTYRNSKAEETLQANYEKTLLRFETTPQPTII
                     DVKLDLDLQPHARRLETRGFYVVQNNTGAPLGEMHLRWNEDLKMNAITVQGARMVREW
                     KEFDYRIYRFDTPMQPSERRTVSFDTVMEQRGFKNSGNQTDIVANGTFTNNMDFAPMI
                     GMSRMGGLLSDRAKRRKFGLPPELRPAKLEDLSATRRNYIGAD"
BASE COUNT          656 a         1189 c         1196 g          709 t
ORIGIN      
        1 gcgctggaaa cggcctcgtt cgatgcagct gtcgcggtct cgcccatgac gatgacgacg
       61 ctggccaggg ccgcgaccac gcttagaggg agaacggccg agcccgcgtc atccagttct
      121 gcggctctct tgcggatctg accgaccgac tggatacgca ccaccttgat catggtgatc
      181 aacaggaaca atgcgacgcc cgcgtcccaa cccgaagcca tccgcgtcgg ccagcgccag
      241 cttgccggcg tcaacaaccc aaccgcgact gcgcaggcga gggccagtag caggggccag
      301 tgcagatgga tcagacgttt gggcgacggc atgggcgata ctggttcggt gggagcgggg
      361 ccggcagcct gaacccatcc gcagaaaaag cgaaggcggc gtcggattct tacaagtcgg
      421 taacttgccg ggcccccgcg gtggtgacaa ggttgcttga ctagggtgag gccgggcgca
      481 aaaccggctc aggaacattg gggctgtcgc atgctgtcga tcaagaatct cgtccacgtc
      541 tatggcaacg ggacaagggc gctcgatgat gtcagcctgg aaatcccgac cgggatgttc
      601 ggcctgctgg gcccgaacgg cgctggcaag tcgacgctga tgcggtcgct ggcgacgctg
      661 cagaccccga cctcgggaac gatcgacttc gacggcatca atgtgatcaa ggatcccgag
      721 aagctgcggc gcacgctcgg atacctgcca caggatttcg gcgtctatcc gcgcgtctcc
      781 gcctacgaca tgctggatca tatggcggtg ctcaagggca tcgcctcggg caaggaccgc
      841 aaggcgacgg tcgagagcct gctgcagcag gtcaacctct ggcccgtgcg caacaaggct
      901 ttggccgggt tctcgggcgg gatgcgtcag cgcttcggca tcgcccaggc gctaatcggc
      961 aatccgaaac tgatcatcgt cgatgagccc acagcaggcc tcgaccccga ggaacgcaac
     1021 cgcttcctca atctgctggc cgagatcggc gagcaggtgg tgatcatcct gtcgacccac
     1081 atcgtcgagg acgtcgccga tctgtgcccg cgcatggcgg tgctggcggg cggcaagatc
     1141 cagttgcaag gcgcgccgca agaactgctc aagcagatgg agggccgcgt ctggaaaaaa
     1201 accatcgcca aggaggcgct ggaggatcac cggacgcgcc atgatgtcat ctccacgcgc
     1261 ctcttcgccg gccagacggt catccacgtg ctggcggatc gccgcccggg cgaaggcttc
     1321 gagccggtct ccggcgggct tgaggacgtc tatttctcga ccctgaccgc ctcgcgcaaa
     1381 gtcgcggcgt aagagctgat catgttcctc aaggtcgcca gtttcgaatt ccgctaccag
     1441 ctgcgccagc cggcgttctg ggtcatcgcc atcatcttca ccctgctggg cttcggcatc
     1501 gtcgccgcgc ccggcaacgt cagcatcggc ggcgggggca acgtctacaa gaacgcgcct
     1561 tacattttgt cgatcctgaa catggtgatg gccaccttct tcatgctcgg ctcgacggtg
     1621 atcgtggcca acgtcgtggt ccgtgacgtc cagtcgggtt tcggtccgat gatccagtcc
     1681 acgcggctgt cgaagttcga ctatctgttc ggtcggttca ccggcgcctt cgccgccacg
     1741 gcgctgtgct tcctcagcgt ctcgcttggc gtgctggccg ggacgctgat gccatggatc
     1801 gaccgcgagt tggtcggacc gttccgtccg ggtgactatg tctacgccta cctcatcatg
     1861 ggcttgccgg gggtcttcct gagctcggcg ctgttcttcg tactggcgac gctgacccgt
     1921 tcgatgatgg cgacctatgt cggggtcgtc gccttgctga tcgtctacct gaccgcgacc
     1981 gggaccctgg gcagccgtcc ggagttcgag gtcgccatgg cctgggccga gccgttcggc
     2041 tccggagcct atgggctggc gaccaaatac tggacggcgg tagagaagaa cacgttgaac
     2101 gcgccgctgg tcggggtgct gttgtggaac cgggtgatct ggaccgccgt ggccgccgcc
     2161 gtgctcgcct cggcctactg gctgtatcaa cccgccctgc gcggcaagaa ggccaagacc
     2221 caggacaagc tgcgcgccat cgccgaagcc gcccccgcgc cggctccact cggcatcctg
     2281 ccgtcgccta cttacggcct ggccagcgcc tggacccagt tcgccaagcg cacagctttc
     2341 gagatgtcga tggtgttcaa gtcacccgcg ctggcgatcc tgctggtgct cggcctcggc
     2401 ttctcgctgg tgaccctgct gttcgccggc gagatctatg gaacgccgac gctgctggtg
     2461 acccggatgg tcgtccagac gctgatgggc tcgttcggcc tgatctcgat gatcgtggct
     2521 atctactatt ctggcgaact ggtctggcgc gaccgtgagc gcaaggcgca ggagattgtc
     2581 gattcctcgt ccgcgccgga ctggaccttc ctgatcccca agacgatggc gctgattctg
     2641 gtgctgttct cgatcctgct ggtcggcatc ctgtcgggcg tcgtgatcca gaccttcaag
     2701 ggctacacca actacgagtt cggcaaatac ctggcctggt acctcgtgcc cgagacgctg
     2761 gactatgccg tgctggcggt cctggcgatc ttcatccagt cgctgtcgcc gaacaagttc
     2821 gtcggctggg ccatcatggt cctgtatctg atctcgacga tcgtcgcgtc aggtcttggc
     2881 ttcgaccacg tgctgtatcg ctatggctcc ggcattggcg ggcccctgtc ggacatgaat
     2941 ggctcgggcc atttccgcgg cttctccaac tgggtggatg cttactggtc cgccgccgcc
     3001 gtgatcctgc tgacgctggc ctatggtctg tggcgacggg gaacggagac ccggttcatg
     3061 ccgcgcctga agcgcctgcc tcggcgactg aaaggcacgg ctggcgtcat cggcggcgtc
     3121 gctgtcgcgg ccttcatcgg cctcggcgtg ttcatcttcg tcaacaccaa cgtctggaac
     3181 acctaccgga actccaaggc cgaagagacg ctgcaggcga actacgagaa gacactgttg
     3241 cgcttcgaga ccacgcctca accgacgatc atcgacgtga agctggatct cgatctgcag
     3301 ccgcacgcgc ggcgtctgga aacgcgaggc ttctatgtgg ttcagaacaa cacaggcgct
     3361 cctctgggtg agatgcacct gcgctggaat gaagatctga agatgaacgc gatcacggtt
     3421 cagggcgcgc ggatggtgcg cgagtggaag gagttcgact accgcatcta tcgcttcgac
     3481 acgccgatgc agccaagcga gcgccggacc gtcagcttcg acacggtgat ggagcagcga
     3541 gggttcaaga acagcggcaa ccagaccgac attgtcgcca acgggacctt caccaacaac
     3601 atggacttcg cgccgatgat cggcatgagc cgaatgggcg gtctgctgag cgatcgcgcc
     3661 aagcgccgca agtttggtct gccgcctgag ctgcgtccgg ccaagctgga agacctgtcg
     3721 gcgacgcgcc gcaactacat cggggcagac
//
LOCUS       DASVTM010000333         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_456662,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000333 DASVTM010000000
VERSION     DASVTM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456662"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VF633_09095"
     CDS             complement(<1..1555)
                     /locus_tag="VF633_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX8471254.1"
                     /translation="MLASIATVAFEGVDARRVDVQVQQIAAGEGSFAIVGLPDKAVAE
                     SRERVRGAFAGIGLALPNRRIIANLAPADLPKEGSHFDLPIALALLASMGVITPDALE
                     GWAALGELGLDGRIAPVGGTLPAAVAAASMGLGLICPEANGPEAAWAGDVSILAPRSL
                     IALVNHFKGTQVLRAPEPGPIKMGVRVADLREVKGQESAKRALEVAAAGGHNLLFIGP
                     PGSGKSMMAARLPGLLPALTPQELLETSMVWSVAGLIERGALTRERPFRSPHHSASMA
                     ALTGGGHKAKPGEASLAHNGVLFLDELPEYSAQALDSLRQPMETGEIIVARANHHVRY
                     PARFQLIAAMNPCRCGVGGFGKGACGKAPRCQRDYQNRISGPMFDRIDLTVEIPPVTA
                     ADMALPPPAEGTAEASARVAVARAMQEERIASLGLNPAQARDQGINARCSGEILDRFA
                     TPDDAGRALLMRAGEAGGLTARGWTRTVRLARTIADLEGSTGVLRRHIAEALIYRRTS
                     VGAQDDFDRQ"
     gene            1704..1970
                     /locus_tag="VF633_09100"
     CDS             1704..1970
                     /locus_tag="VF633_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX8471255.1"
                     /translation="MTQIPVSEAKAQLTDLIRRAEAGEEIIVTRHGQGVVRLTPLRRA
                     ATMTPEQRMKVLREVQQSARGKFEPGFSAARSQDFLYDDDGLPG"
     gene            1967..2350
                     /locus_tag="VF633_09105"
     CDS             1967..2350
                     /locus_tag="VF633_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8471256.1"
                     /translation="MIAVDTSALIAVVAGEEPARACEQILISSELIMSAGTLTEALIV
                     SSRKHVRPELQRLLSVLDLRIENVTPDFARRAAAVYDRWGKGVDSASLNYGDCFSYAV
                     AELHDCPLLFIGDDFSRTDIRSALG"
     gene            complement(2347..3294)
                     /gene="gshB"
                     /locus_tag="VF633_09110"
     CDS             complement(2347..3294)
                     /gene="gshB"
                     /locus_tag="VF633_09110"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX8471257.1"
                     /translation="MLKVAIQMDPVEGVDIEADTTWLMMMTAQERGHQQWVYDFRTLA
                     LEEGRLYCRARPVTLQHVAGDHVTFGEWAKLDLAEDIDVILMRQDPPFDMAYVTSTYM
                     LETVHPGTLVVNDPAEVRSAPEKLMVTGFPGLQPPTLITSDPIALTEFHKKHGDVVLK
                     PLHGNGGSGVIKLRADDPNLDALIEIHQGASRDPLVIQKFIPAVSKGDKRIILIDGEP
                     VGAINRVPAAGAVRSNLHVGGTAMPVELTARDLEICAAIGPTLKERGLIFVGIDVIGD
                     YLTEINVTSPTGAQQLKQFTGIDATALMWDVIEAKRAAA"
BASE COUNT          595 a         1085 c         1086 g          588 t
ORIGIN      
        1 gttggcggtc gaaatcgtcc tgagcaccga cggaggtgcg gcgatagatc agggcttcgg
       61 cgatgtggcg gcggaggacg ccggtcgatc cttcgaggtc ggcgatcgtt cgcgccagtc
      121 gcacagtacg ggtccagccg cgcgcggtca ggcctccagc ctccccggcg cgcatcagca
      181 gggcgcggcc tgcatcatcg ggcgtggcga agcggtcgag gatttcgccg gagcagcggg
      241 cgttgatgcc ctgatcgcga gcctgcgccg ggttgaggcc gagcgaggcg atccgttctt
      301 cctgcatggc tcgggcgacg gcgacgcggg cggaggcctc ggccgtacct tccgctggtg
      361 gcggcaaggc catgtcggcg gcggtgacgg gcggtatctc gaccgtcaag tcgatgcgat
      421 cgaacatcgg acccgagata cggttctgat agtcacgctg acatcgcgga gccttgccgc
      481 aggctccctt gccgaatccc ccgacaccgc agcggcaggg attcatcgcc gcgatcagtt
      541 ggaaccgggc cggatagcgg acatggtggt tggcgcgggc cacgatgatc tcgcccgtct
      601 ccatcggctg gcgcaggcta tcgagcgcct gtgcggaata ttccggaagt tcatccagga
      661 acagcacccc gttgtgggcc agcgacgcct cgcccggctt ggccttgtga cccccgccgg
      721 tcaaggcggc catggaggcg gaatggtgtg gcgaacgaaa aggccgctcc cgggtcaaag
      781 ctccgcgctc gataaggcca gcgacagacc agaccatcga cgtctccagc agttcctgcg
      841 gcgtcagcgc tggcaacagt ccgggcagtc gcgccgccat catcgacttt ccggagcccg
      901 gtggcccgat gaaaagcagg ttgtgaccac cggcggcggc gacttccaag gcccgcttgg
      961 cgctttcctg acccttgacc tcgcgcaggt cggcgacgcg cacccccatc ttgatcggcc
     1021 cgggttcggg cgctcgtagc acctgcgtac ccttgaagtg attgaccagc gcgatcagcg
     1081 agcggggcgc gaggatcgat acgtcgcccg cccaggccgc ctccggaccg ttagcttcgg
     1141 ggcagatcaa cccgagaccc atcgatgctg cggccacggc tgccggcagg gtgccgccta
     1201 cgggcgcgat ccgcccatcc agccccagct cgcccagcgc ggcccaacct tcgagcgcgt
     1261 cgggcgtgat gactcccatc gacgccagca acgccaaggc gatcggcagg tcgaaatgac
     1321 taccctcctt gggcaggtcg gcgggggcga gattggcgat gatccgcctg ttgggcaggg
     1381 ccaatccgat gccggcgaag gccccgcgca cccgctcacg gctttcggcc accgccttgt
     1441 ccgggagacc gacgatagcg aacgagcctt cgcctgctgc aatctgctgc acctgaacgt
     1501 cgacccgccg cgcatccacg ccttcgaacg ccaccgtcgc aatactcgcc agcatggccc
     1561 gttcctctac ctgcagagaa cgaaccatga accaagctgg attgcaagat cagatacaga
     1621 cacgccttgg ttgatcgttg agttggacca aagagtggtc cataatcccg tcgcagaact
     1681 tcagtgtcca cgccgagact acgatgaccc aaatccccgt atccgaggcc aaagcgcagc
     1741 taaccgatct gatccgccgt gccgaagccg gcgaggagat catcgtgacc cggcatgggc
     1801 aaggcgtggt tcgcctaacg ccgctaagga gagccgcaac tatgacgcct gagcagcgaa
     1861 tgaaggtctt gcgtgaggtt cagcaatccg cacgcgggaa gttcgaacct ggcttctccg
     1921 ccgcgcgcag tcaggacttt ctctatgatg acgatggtct tccgggatga tcgcagtcga
     1981 tacatccgcg ctgatcgcgg tggttgccgg agaggaaccg gcacgcgcct gtgagcaaat
     2041 cctcatatcc tccgagctaa tcatgtcggc cggaacgctc accgaagccc tcattgtcag
     2101 ctcgcgtaaa cacgttcggc ccgaactgca gagactgctt tcggtgctcg atctcaggat
     2161 cgagaacgtc acacccgact tcgcccggcg cgcagcagca gtttatgacc ggtggggcaa
     2221 aggcgttgat agcgcctcgc tcaactatgg cgactgcttc agctatgcgg tcgctgagct
     2281 ccacgactgc ccgctgctct tcatcggcga cgacttctcc cgtaccgata tccgcagcgc
     2341 gctgggttag gctgccgctc gcttcgcctc gatcacgtcc cacatcaggg cggtcgcatc
     2401 gatgccggtg aactgcttca gctgttgggc gccggtgggg gaggtgacgt tgatctcggt
     2461 caggtagtcg ccgatcacat cgatgccgac gaagatcagt ccccgctcct tcagcgtcgg
     2521 gccgatggcg gcgcagatct ccagatcgcg ggcggtcagc tcgaccggca tggccgtgcc
     2581 gccgacgtgc aggttcgagc gcaccgcgcc ggccgccgga acgcggttga tcgctccgac
     2641 gggctcgccg tcgatcagga tgatgcgttt gtcgcccttc gacaccgccg gaatgaattt
     2701 ctggatcacc agcggatcgc gtgaggcgcc ctgatggatc tcgatcaggg cgtcgaggtt
     2761 ggggtcgtca gcgcgcagtt tgatcacgcc agaaccaccg ttgccatgca ggggcttcag
     2821 caccacgtcg ccgtgcttct tgtggaactc ggtcagggcg atcgggtccg aggtgatcag
     2881 ggtcggaggc tgcaggccgg ggaagcctgt gaccatcagt ttctcgggcg cggaccgcac
     2941 ctcggccgga tcgttgacca ccagcgtgcc cggatgaacc gtctcgagca tgtaggtcga
     3001 agtgacatag gccatgtcga agggcggatc ctgacgcatc aggatgacgt cgatatcctc
     3061 ggccagatcc aactttgccc actcgccgaa ggtcacgtga tcgcctgcaa cgtgctgcag
     3121 cgtcacggga cgggcgcggc aatacagacg gccctcttcc agcgcgaggg tgcggaaatc
     3181 atagacccac tgctgatggc cacgctcctg cgccgtcatc atcatcagcc aggtcgtatc
     3241 ggcctcgata tcgacccctt cgacggggtc catctggatc gcgaccttga gcatgtttgg
     3301 ggaccttgcg ccgttggaag cttgggtaat gggtcgagag gggggcgggc gcaa
//
LOCUS       DASVTM010000334         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_937718,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000334 DASVTM010000000
VERSION     DASVTM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937718"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VF633_09115"
     CDS             complement(<1..1289)
                     /locus_tag="VF633_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX8471258.1"
                     /translation="MRTAYVTARPRYGLADIVGLMFRELLLMIIIFLVVFAVGAAAVM
                     TLKKTYTATAEVYAGAGQEYVYESRIGTVTERASPPPSGEIAQTEANILTSRDVKLRA
                     VRALGLSTFHAPGKPATGSLTQQEGDAIRAITEGLAVGTSPTSSIISLSYESDNAEKS
                     AKVLAAILESYKAYRREVFQNNSTEAIDTQRQTFQNELGDVDDAYERFLSSNDIGDFT
                     TAKASAAATYQTTFADRLSTQAQLSQAARRVETITAQLSAVPAEIVLQQDLNIAAQDQ
                     ILQKRTERETLLARYRPDSQPIRDIDAQIAQLQAYVAGGDTVGAKEVRTGPNPSWVSL
                     DTARSTAEADRDALAARLAMLDQQMSALSERQARLTRLESENAILSGNRDVLTANIKE
                     FQQRGTQSRADNDLVASGADNVRILSHPAAPTKGSSL"
     gene            1414..>1723
                     /locus_tag="VF633_09120"
     CDS             1414..>1723
                     /locus_tag="VF633_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX8471259.1"
                     /translation="MTADRRQILFALGAVSAASALSACGVTSGLPLVDQGPPSGNRIE
                     TGNFPEIGFSDWTDQEPEYLFYPGDELEVATPTATELTRTVKVGPVGRIALPLIGQMM
                     A"
BASE COUNT          266 a          527 c          591 g          339 t
ORIGIN      
        1 ttgaggctgg agcccttggt tggggctgcg ggatgcgaca ggatgcggac attgtcggcg
       61 cccgaggcca ccagatcgtt gtccgcccgg ctctgggtgc cgcgttgctg gaattccttg
      121 atgttggccg tcaggacgtc gcgattcccg gacaggatgg cgttttccga ctccagccgg
      181 gttagtcggg cctgacgctc gctgagcgcc gacatctgct ggtccagcat ggccagacga
      241 gcggccaagg cgtcgcggtc cgcctcggcc gtggagcgcg cggtgtccag cgacacccag
      301 ctcgggttcg gcccggtgcg gacctccttg gcgcccacgg tgtcgccacc cgccacataa
      361 gcctgcagct gggcgatctg ggcgtcgatg tcgcggatcg gttgcgaatc gggccggtag
      421 cgggcgagca gcgtttcgcg ttcagtgcgc ttctgcagga tctggtcctg cgccgcgatg
      481 ttcaggtcct gctgcagcac gatctcggcg ggtacggcgg agagttgggc ggtgatcgtt
      541 tccacccggc gcgcagcctg gctcagctgt gcctgggtgg agagccggtc ggcgaaagtc
      601 gtctggtagg tcgccgcagc ggaggccttg gccgtggtga agtcaccgat atcgttggag
      661 gacagaaacc gctcgtaagc gtcatcgaca tcgccgagtt cgttctggaa ggtctgccgc
      721 tgggtgtcga tcgcttccgt cgagttgttc tggaagacct cgcggcgata ggccttgtag
      781 ctctcgagga tggcggccag caccttggcg gatttctccg cgttatcgct ctcatagctc
      841 agcgagatga tcgaactggt cgggctggtg ccgacggcca gaccttcggt gatggcgcgg
      901 atggcgtcgc cttcctgctg ggtcagtgaa ccggtcgctg gcttgcccgg tgcgtgaaag
      961 gtgctgaggc ccagggcccg gacggcgcgc agcttgacgt cgcggctggt cagaatgttg
     1021 gcttcggtct gcgcgatctc gcctgacggc ggcggactgg cccgctcggt gaccgtgccg
     1081 atgcgcgatt cgtagacata ctcctgcccg gcgccggcat agacctcggc ggttgcggta
     1141 taggtcttct tcagcgtcat cacggccgct gcgccgacgg cgaagacgac caggaagatg
     1201 atgatcatca acaggagttc gcggaacatc aggccgacga tgtccgcaag gccataacgt
     1261 ggacgggcgg tcacataggc cgtgcgcatg gggcgagcga atccatcgga tgagatactg
     1321 atgcgatcct tggctcaccc gcgttaacca ctggttaccc ataaaccgcc agtgtcccgc
     1381 ctatccgctc acaatatcgg ccgctgacgt tccatgaccg ctgaccgccg ccaaatcctg
     1441 ttcgccctcg gcgccgtatc ggcggccagt gcgctgtccg cctgtggcgt gacgtccggt
     1501 ctacccctgg tcgatcaggg gccgccgagt gggaatcgca tcgaaacggg caattttccg
     1561 gagattggct tctccgactg gaccgaccag gagccagaat acctgtttta tcccggcgat
     1621 gagctcgagg tcgccacccc gacggcgacc gagctgaccc gcaccgtcaa ggtcgggcca
     1681 gttgggcgaa tcgccttgcc cctgattggt cagatgatgg cgg
//
LOCUS       DASVTM010000335         8983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_617250,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000335 DASVTM010000000
VERSION     DASVTM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617250"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VF633_09125"
     CDS             complement(<1..1412)
                     /locus_tag="VF633_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX8471260.1"
                     /translation="MFGKRTGQPGTSAPESRATPAQHGGTAQPQQGVQTQAFAYADDP
                     HDSPTADGPAQGAGAQGGGAQGGLTTAGFAQKNASRPASPAADRLDALAARPGAATAP
                     APKASSGPGPRATAGLEQLKKAQPVAEIVREQSDYYHATKTTIFNALMNTIDLSQLAQ
                     LDTKAASEEIRDIVAELVAIKNVSMSVAEQEHLVQDIVNDVLGYGPLEPLLARDDIAD
                     IMVNGAGRVFIEVNGKVQLTNVRFRDNTQLMNICQRIVSQVGRRVDESSPICDARLPD
                     GSRVNVIAPPLAIDGATLTIRKFKKDKLTMKNLVEYASISPEGARVLGVIGACRCNVV
                     ISGGTGSGKTTLLNTLTAFIDPTERVITCEDAAELQLQQPHVVRLETRPPNLEGQGAI
                     HMRDLIKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANSPREALARME
                     SMITMGGYGLPSKTIREMITG"
     gene            complement(1422..2942)
                     /locus_tag="VF633_09130"
     CDS             complement(1422..2942)
                     /locus_tag="VF633_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HEX8471261.1"
                     /translation="MSNAFAPRSYEAFEDEFDLDLEFAPQSPPAGGGEPDVREPLQFT
                     APGVSDPLITPSSASGQMPVAASGFNPVGDLVASAASALAPIGGPMAAEVSVPRIAIH
                     VFAERQDTLAAAERAGQDRRLSRATTQIRVGGVPAAVEAYHHEPTPPLIIVECLSDPH
                     TLLQQVDTLAEVCDAGTKVVVIGATNDIILFRELMRRGVSEYLVAPIQPLQLIAAIGG
                     LFADPAQPFIGRSIAFVGARGGAGASSVAHNTAYAMSERIGANTVIVDYDLPFGTAGL
                     DFNQDPLSGVADALGQPDRLDATLLDRMMVRCTDKLSLFAAPATLDVDWDISPDAFEE
                     VTSRIRSTAPFVVLDLPHLWSGWMRKTLITADEVVVVATPDLASLRNAKNMIDLIRTG
                     RPNDAPPRLVLNQVGVPGRPEIPAKDFGAALGVIPSLIIPFDAKLFGAAANNGQMIID
                     AGAKSKAAEAFQTLAQIVSRREMPQVAGPRARSTQSGSPTGKPESKSLFGSMFKKR"
     gene            complement(2950..3630)
                     /locus_tag="VF633_09135"
     CDS             complement(2950..3630)
                     /locus_tag="VF633_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEX8471262.1"
                     /translation="MISNSRLLSVAAALALSSALSACMGAQAGSLSPVPLTPTQRFTL
                     QVEPGVERIALAVHETGLTANQTAALSSLANSFGIEGAQVLRIEAPSGDDPVSADFAY
                     RIKAELEHRGIGPVQVVAYSAPDPRAPVLVGYETLRAMVPKCGTQWESLTRTGSNQTP
                     SNFGCAVTANLAAQIDNPRDIVQPRGMTPSDAGRRTVVFDNYRKGEATAATQEELVAN
                     QRVSQAVN"
     gene            complement(3639..5240)
                     /locus_tag="VF633_09140"
     CDS             complement(3639..5240)
                     /locus_tag="VF633_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX8471263.1"
                     /translation="MKLIKTPARGLPIRALKVWASVSVIALGNLAPFAAQAQTFAASA
                     DTGGRSSVQVGATPRLINLPRGSSMAIDLPTDAHDAIVSNPAVAEALTHSSRRYTIIG
                     IAPGQTDAVFLDAAGRTILSLRIRVDAGVYALQDTINRVAPGSQAHAEALNDSIVLTG
                     LVTSPAEADRIVQLSRAFVSAPEKVINMLTVAGSDQVTLKVRVVEIQRNAIKQLGFDT
                     NAVLGQVGQTQFLLGQAATFGVNGALLGGISSGISRDTTKNFQQQRPCGGLFPAGALC
                     PVVVNGPEDSANWDTAQPGTGPGSNDLNKGSATIKAFERVGLIRTLAEPNLTSVNGES
                     ASFLAGGEFPVPTGRDREGNISVEYKPYGVSLSFRPIVLSGGRISLQTSVEVSELSNT
                     GGLTIGAGTASSITLQGLSVRRVQNTVELPSGGSMMIAGLLQETTRQNIDSLPGVMNL
                     PVIGSLFRSRDYLSGETELVVIVEPYIVSPTSPGRMQTPADGLRIATDVQTNLFGQLN
                     QTYGTPAPTAAGGTGWQGPVGYVIE"
     gene            complement(5249..6196)
                     /gene="cpaB"
                     /locus_tag="VF633_09145"
     CDS             complement(5249..6196)
                     /gene="cpaB"
                     /locus_tag="VF633_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX8471264.1"
                     /translation="MKPARIAVICIAAVAAIGLAFVVRAMGSSGKPVAVAAAAAPVEV
                     RPMAKVLVAARDLQPGKRLEDADLEWKDWDVNEVNPAFITDGSTPLPAPPTTAEAKAA
                     AGTLEGASAAIGNKPGNAIAAVARAANSVATSGGKADYFGSVVREPILKGEPIVSRKI
                     VRAGDSGYLAAYLEPGMRAMAIRVTVETAAGGFILPGDRVDVLLTRPTTTSGVEGGQD
                     SKFAAATVMRNIKVLAIDQTTRAGEDEQSVVGATATMEVGPVDAEALALAKAQGELSL
                     VLRSYADTGGPSGRVAQVQDQSTAVRIFRGGAPEVVVVQ"
     gene            complement(6352..6867)
                     /locus_tag="VF633_09150"
     CDS             complement(6352..6867)
                     /locus_tag="VF633_09150"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8471265.1"
                     /translation="MEILTLILLSTLPCLVIAGGLRDLTTMKIPNWISVALILCFFPT
                     AFLVGLPPMTVAIHFGVAVVALLVGMGLFAMRVIGGGDAKLMAAVCLWLGITGSGMFI
                     LWTALAGGLVCVVLILARRELRPYVMGAPAWIDTLLEPKGDLPYGVAVAIGALMAFPS
                     SILLTTFSASH"
     gene            complement(7013..7195)
                     /locus_tag="VF633_09155"
     CDS             complement(7013..7195)
                     /locus_tag="VF633_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8471266.1"
                     /translation="MTKFISKFVQDESGATAIEYGLIAALISVAIIGAVTALGTNLTG
                     AFGRVSSALGTAGTAA"
     gene            7494..7961
                     /locus_tag="VF633_09160"
     CDS             7494..7961
                     /locus_tag="VF633_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEX8471267.1"
                     /translation="MFARIALRAGYIAMTVTTRRLALPAVSALVAVLAMSVAGPGLTQ
                     SSVMDLVVDTSQRVAVRGAISSIIVANPQIADATVVDANTLFVTGKGYGSTQITAVDA
                     IGRILFQNQIVVTGGQAGAVRVWRGGRVTEMACAASCSPSVRSDGGASATPTP"
     gene            7976..8545
                     /locus_tag="VF633_09165"
     CDS             7976..8545
                     /locus_tag="VF633_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8471268.1"
                     /translation="MKPGTPGARKPHARSREGATAVEFAMIAFPFFFMLFAILEIGLL
                     FVTDSMLENAVIETGRLIRTGVADNSRMTADQFKASLCTRMSIFSADCASQATVDVRV
                     ITQFRNVAPPDPLANGTSFDKSGLTYLTGQPGSLVLIRVWYSRPLITPFMAQALSKLG
                     DGNTILTATTTFRNEPFNAATPLPAPATP"
     gene            8542..>8983
                     /locus_tag="VF633_09170"
     CDS             8542..>8983
                     /locus_tag="VF633_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8471269.1"
                     /translation="MIRESLPAFIVRLARDRRGVSAVEFALIAPVMIAFYFAMGELCQ
                     GYMAQKRLSHTASSIANLVAQTDVVKEDEVADVMAIGALIMKPFPATSLSTRITSVTR
                     DANGVAKVDWSRASGAGLPAYADQAVVTVPTDMIGNGASLVMAEA"
BASE COUNT         1573 a         2731 c         3081 g         1598 t
ORIGIN      
        1 ccggtgatca tttcgcggat cgtcttcgaa ggcaggccat agccacccat ggtgatcatc
       61 gactccatcc gggcaagcgc ctcgcgcggg gagttggcgt gcagcgtacc catcgagccg
      121 tcgtgaccgg tgttcatggc ctggagcagg tcgaacgcct cggggccccg gacctcgccg
      181 acgatgatgc gttcggggcg catccgcagg cagttcttga tcaggtcccg catgtggatg
      241 gcgccctgac cctcgaggtt cggggggcgg gtctccaggc ggacgacgtg cggctgttgc
      301 agctgcagtt cggcggcgtc ctcgcacgtg atgacgcgtt cggtcgggtc gatgaaggcg
      361 gtcagggtat tcagcagcgt cgtcttgcca gaacccgtac cgccggagat gacgacgtta
      421 cagcggcagg cgccgatgac gcccagaacg cgggcccctt ccggactgat ggaggcgtac
      481 tccaccaggt tcttcatcgt cagcttgtct tttttgaact tccggatcgt cagggtggcg
      541 ccgtcgatcg caagaggcgg ggcgatgacg ttgacgcgcg agccgtccgg cagacgtgcg
      601 tcgcagatcg gagagctctc atcgacgcga cgaccgacct gcgacacgat ccgctggcag
      661 atgttcatca gctgggtgtt gtcgcggaag cggacgttgg tcagctggac cttgccgtta
      721 acctcaatga acacccggcc ggcgccgttg accatgatgt ccgcgatgtc gtcacgcgcc
      781 agcagcggct ccaacgggcc gtagccgagg acgtcgttga cgatgtcctg aaccagatgc
      841 tcctgctcgg ccacggacat ggagacgttc ttgatcgcca ccagttcggc gacgatgtcg
      901 cgaatttcct cggaagcggc cttggtgtcg agctgggcca actgggacag gtcgatggtg
      961 ttcatcaacg cgttgaagat ggtggtcttc gtggcgtggt agtagtcgga ctgctcgcgc
     1021 acgatctccg cgaccggctg agccttcttc aactgttcga gaccggccgt agcgcgcgga
     1081 cccgggccgc tcgacgcttt cggagctgga gcggtagccg cacccggacg agcggcgagc
     1141 gcgtccagcc ggtcagcggc aggcgaagcg gggcgtgaag cgttcttttg ggcgaaccca
     1201 gccgtggtca agccgccttg agccccacca ccctgagccc cagcaccctg agccgggccg
     1261 tcggccgtcg gactatcgtg cgggtcgtcg gcgtaggcga aggcctgggt ctggacgcct
     1321 tgttgcggtt gggccgtgcc gccatgctgg gcgggcgtcg ctcgcgattc cggggccgag
     1381 gttccgggct gacctgtgcg tttaccgaac acgatcgacc gtcagcgctt cttgaacatt
     1441 gagccgaaga gcgatttgct ctcgggtttg ccggtgggcg aaccgctctg ggtcgaccgc
     1501 gccctgggac ccgcgacctg gggcatctcg cgacgcgaga cgatctgggc cagagtctgg
     1561 aaggcttctg cagccttgga ttttgccccg gcgtcgatga tcatctgccc gttgttggcg
     1621 gccgcaccga acagcttggc gtcgaagggg atgatcaggc tgggaatgac gccgagagcg
     1681 gcgccgaaat ccttggccgg gatctccggg cgaccgggaa cgccgacctg gttgagcacc
     1741 agacgcggcg gtgcgtcatt gggacgcccc gtcctgatca gatcgatcat gttcttggca
     1801 ttgcgaagcg atgccagatc cggcgtggcc actacgacca cctcatcggc ggtaatcagt
     1861 gtcttgcgca tccagccgga ccagaggtgg gggagatcca gcaccacgaa gggggcggtg
     1921 gagcggatgc ggctggtcac ttcttcgaag gcgtcgggag agatgtccca gtcgacgtcg
     1981 agggtggcgg gcgcggcgaa cagcgacagc ttgtcggtgc agcgcaccat catccggtcc
     2041 agcagggtcg catcgaggcg gtcgggctgg cccagcgcat cggccacacc gctcaacgga
     2101 tcctgattga agtcgagacc tgcggtgccg aacggcaggt catagtcgac gatgaccgtg
     2161 ttggcgccga tccgctccga catcgcgtag gcggtattgt gcgccaccga cgaagctccg
     2221 gccccaccgc gcgcgccgac gaacgcgatc gagcggccaa tgaaaggctg cgcggggtcg
     2281 gcgaacaggc caccgatggc ggcgatcagc tgcaggggct gtatcggggc gaccagatat
     2341 tccgagacgc cgcgacgcat cagctcgcgg aacaggatga tgtcgttggt agcgccgatg
     2401 accaccacct tggtgccggc gtcgcaaacc tccgcgagag tatcgacctg ttgcaacagg
     2461 gtatgcgggt cgctgaggca ttccacgatg atcagcggcg gcgtgggttc gtggtggtag
     2521 gcctcaacgg ctgcgggcac gccaccgacg cggatctggg tcgtggcgcg cgacaggcgg
     2581 cggtcttgac ccgcgcgttc ggcagcagcc agcgtgtcct gacgctcggc gaagacgtgg
     2641 atcgcaatgc gcggcaccga cacttcggcc gccataggcc ccccgatcgg cgcgagggct
     2701 gaggccgcgc tcgcgacaag gtcgccgacg gggttgaaac cagacgcggc gaccggcatt
     2761 tgcccgctcg ccgaagaggg agtgatcagc ggatcggaga cgcccggcgc cgtgaactgc
     2821 agcggctcgc gcacgtccgg ctcgccccct ccggcgggcg gggactgggg agcgaactcg
     2881 aggtcgagat cgaactcgtc ctcgaaagcc tcgtagcttc gtggtgcaaa ggcgttgctc
     2941 atgctcggat caattcaccg cctgcgagac tcgctgattt gcgacgagtt cctcttgagt
     3001 ggccgccgtg gcttcgcctt tgcggtagtt gtcgaaaacg acagtgcgtc gaccggcgtc
     3061 cgaaggtgtc atgccgcgtg gctggacgat gtcgcgggga ttgtcgatct gggcggccag
     3121 gttcgccgtc accgcgcagc cgaaattgct cggcgtctgg ttggaaccgg tccgtgtcag
     3181 gctctcccac tgggttccgc atttgggaac catggcgcgc aatgtctcat agccgacgag
     3241 tactggcgcc cgtggatcag gggcgctgta ggcgacgacc tgaaccggac caatgccgcg
     3301 atgctcgagc tcggccttga tccggtaggc gaagtcggcg gagacgggat cgtcgccaga
     3361 cggggcctcg atgcggagga cctgagcgcc ctcgatgccg aagctgttgg cgagcgagga
     3421 cagggcggcg gtctgattgg cggtcagccc cgtttcgtgc accgccagag cgatgcgttc
     3481 gacacctggc tcgacctgca gtgtgaaacg ctgggtcggg gtcaggggaa cgggcgacag
     3541 gctcccggcc tgggcgccca tgcaagcgga gagcgcggat gacagggcca aagcagcggc
     3601 caccgaaaga agacgggagt tggagatcat ggggcgcctc actcgatcac atagccgacc
     3661 ggaccctgcc agccggtgcc gccggcggcg gtcggggcgg gggtgccgta ggtctggttc
     3721 agctggccga acaggttggt ctggacgtcg gtggcgatgc gcaggccatc ggcgggcgtc
     3781 tgcatccggc cgggcgaggt cgggctgacg atatagggct cgacgatcac gaccagctcg
     3841 gtctcgccgc tgagatagtc gcgcgaccgg aacagggagc cgatgaccgg caggttcatg
     3901 acaccgggca aggagtcgat gttctggcgg gtcgtttcct gaagcagccc ggcgatcatc
     3961 atagagccgc cgctgggcag ttcgaccgta ttctggaccc ggcgaacgct cagaccctgg
     4021 agggtgatcg acgacgccgt gcccgcgccg atggtcaggc cgccggtgtt cgacagctcg
     4081 gagacctcga ccgaggtctg cagcgagatc cggccgccag acaggactat cggccgaaac
     4141 gacaggctga cgccgtaggg cttgtattcg acgctgatgt ttccttcccg gtcgcgaccg
     4201 gtcggcaccg ggaactcgcc gccggcgagg aagctggcgg actcaccgtt gaccgatgtc
     4261 aggttgggct cggccagggt gcggatcaga cccacgcgct cgaacgcctt gatcgtggca
     4321 gaacctttgt tcaggtcgtt tgatccgggg ccggtgccgg gctgggcggt atcccagttg
     4381 gccgagtcct cgggcccgtt gacgactact gggcaaaggg cgcccgccgg gaacagtccg
     4441 ccacaaggcc tctgttgctg aaagttcttc gtggtatcgc gcgatatacc gctcgatatt
     4501 cctcccagca gggcgccatt gacgccaaat gtggcggctt ggcccagcag gaattgcgtc
     4561 tgaccgacct ggccgagcac tgcattcgtg tcgaagccga gttgcttgat ggcgttgcgt
     4621 tgaatctcga cgacgcggac tttgagcgtc acctgatccg aacccgcgac ggtcagcatg
     4681 ttgatgacct tctcgggcgc cgagacaaag gcgcgagaga gctggacgat gcggtcggct
     4741 tcggcaggac tggtcaccag cccagtcagg acgatgctgt cgttgagggc ttcggcgtgg
     4801 gcctgggacc ccggcgcgac gcggttgatg gtgtcttgca gggcataaac cccggcgtcg
     4861 acacggattc gcagcgagag gatcgtgcgg cctgcagcgt cgagaaaaac ggcgtcagtc
     4921 tgacccggcg ctatgccgat gatggtgtag cggcgcgaac tatgcgtcag ggcctcggct
     4981 acggccggat tggagacgat ggcgtcatga gcgtccgtcg gcaggtcgat ggccatcgac
     5041 gaaccgcgcg ggagattgat cagacgcggc gttgctccga cctgaaccga agatcggcct
     5101 ccggtatcgg cactcgcggc gaaagtctgt gcctgggccg cgaatggggc gaggttgcca
     5161 agggcaatga cactgaccga tgcccaaacc ttcagggcgc ggatcgggag gccacgggca
     5221 ggggtcttga tgagtttcat tgtgttgctc actggaccac gacgacttct ggagcgccgc
     5281 cgcggaagat gcgaaccgcg gtggactggt cttgaacttg ggccacgcgg cccgatggtc
     5341 cgccggtgtc agcgtaggat cggagcacca gcgaaagttc gccctgagcc ttggcgagcg
     5401 ccagagcttc ggcgtcgaca gggcccactt ccatggtcgc cgtagcgccg actaccgact
     5461 gttcgtcctc gccggcgcgc gtggtctggt cgatggccag aaccttgatg ttgcgcatga
     5521 ctgtcgcggc ggcgaacttc gaatcttgtc cgccttcgac gccggatgtg gtggttgggc
     5581 gggtcagaag cacgtcgacc cggtcacctg gaagaatgaa gccgccggcg gccgtctcga
     5641 cggtgacccg gatggccatc gcgcgcatgc ctggctcaag ataggccgca aggtaaccgc
     5701 tatcgcccgc acggacgatc ttgcgagaga cgatgggttc gcccttgagg atgggttcgc
     5761 gcacgaccga cccgaaatag tccgccttgc cgccgctggt ggcgacactg ttggctgcgc
     5821 gggcgacggc ggcgatggcg ttgcccggct tgttgccgat ggcggcagac gcgccttcaa
     5881 gggttccggc ggcggccttg gcctcggcgg tggtcggcgg cgcaggcagg ggggtcgaac
     5941 cgtcggtaat gaaggcggga ttgacctcgt tgacgtccca gtctttccat tccaggtctg
     6001 cgtcttcgag gcgcttgcca ggctggaggt cgcgcgcagc gaccagaacc ttggccatgg
     6061 gccggacttc gacaggggcg gcggcggcgg ccacggcgac gggcttgccc gacgagccca
     6121 tggcgcgcac gacgaacgcc aggccgatgg ccgcgacggc ggcgatgcag atgacggcga
     6181 tacgggcggg cttcatcgac ggtctccgga acagtcgagc gacgcggaat cggtctgcgg
     6241 acgctgtgcc gttaacgaga atgaaggtcg cgggttaacg agcgcctaaa ccaagttatt
     6301 ccgttcaaac aacttatcgg cggttctccg agccgcgccg cgccgccgtg gttagtgact
     6361 ggcgctgaac gtcgtcagga ggatgctgga agggaaggcc atcagcgcgc caatggcgac
     6421 cgcgacgcca taaggaagat cacccttggg ttccagcagc gtgtcgatcc acgcaggagc
     6481 tcccatgacg taaggtcgca gttcgcgacg cgcgaggatc agcacgacgc acacgaggcc
     6541 accggcgaga gcggtccaca agatgaacat gcctgatcct gtgatgccca accacaggca
     6601 gacggcagcc atcagcttgg cgtctccgcc cccgatcacc cgcatcgcga aaaggcccat
     6661 gccgaccaga agagccacga cggctactcc gaaatggatt gcgaccgtca tcggcggtaa
     6721 cccgaccaga aaagcggtcg gaaaaaagca gaggatcagc gctaccgaaa tccagtttgg
     6781 aattttcatc gtcgtcaggt cgcgcaagcc accggcaatg accaggcagg gcagggtgct
     6841 aagcaggatc agggtcagga tttccatgac atcgacagtt gcctggacag ggttaaggcc
     6901 gggttgagta cacttgccga aggcgatttt ctacggaggc ttgccgtaag catcatacga
     6961 aaagggccgg agcgtttccg ctccggccct tcgcttcaga cctccggaga ggttaggcgg
     7021 cggtgccggc tgtgcccagt gcagaagaga cgcggccgaa agcgccggtc agattggtgc
     7081 cgagagcagt gacggcgccg atgatggcga cagagatcag agcggcgatg aggccgtact
     7141 cgatggcggt cgcgcccgac tcgtcctgaa caaacttgga gatgaacttg gtcatttgga
     7201 tgatccctgt agagaggttg atttagcgag caacgagtga tgggggagag accggttgcc
     7261 cgcttgcccc caacgcctgg agaccatgcc cccacagccg ttaaggagtc gtcggcagtg
     7321 ttggttactg ggacgtttac tataatcagt gcgctccgac acaagtgcag gcctgcaaga
     7381 aggccctgtg ggcaccggaa acctccggcc agagacagta ctgatgctct gttgctacct
     7441 ggccgagctc tgctgcgggg atgatttcag actttattga ggtttcggcg gtcatgttcg
     7501 cgcgtatcgc cctgcgcgcc ggatatatcg ccatgaccgt caccacccgc cgcctcgccc
     7561 ttcccgcagt atccgctctg gtcgccgtcc tggcgatgtc ggtcgcgggt cccggcctca
     7621 cccagtcctc ggtgatggat ctcgtggtcg acacttcaca gagggtcgcc gtgcgggggg
     7681 ccatcagttc gatcatcgtc gccaatccgc agatcgctga cgcgactgtg gtggacgcca
     7741 acaccttgtt cgtcaccggc aagggttacg gttcgacgca gatcactgct gtggacgcta
     7801 tcggtcgtat cctgttccag aaccagatcg tcgtaacagg cggtcaggcc ggcgcggtgc
     7861 gcgtctggcg cggcggccgg gtcacggaaa tggcttgcgc cgcgtcctgc tcgccaagcg
     7921 tccggtccga cggtggcgca tccgccactc cgaccccttg agcgcgatga gggcaatgaa
     7981 acccgggaca cccggcgcca ggaagccgca tgcgcgttcg cgtgagggtg cgacggcggt
     8041 cgagttcgcg atgatcgcct tcccattctt cttcatgctg ttcgccattc tcgagatcgg
     8101 cctgttgttc gtgacggact cgatgctgga aaacgctgtg atcgagaccg gccggctgat
     8161 ccgcaccgga gtggccgata attcgcgtat gacggcggac cagttcaagg cctcattgtg
     8221 cacgcggatg agtatcttca gcgccgattg cgcgtctcag gctacggtcg acgttcgggt
     8281 catcacgcaa tttcgcaacg tggccccgcc agatcccttg gccaacggca catctttcga
     8341 caagtccggc cttacctatc tgacgggcca gccgggcagt ctggtgttga tccgcgtctg
     8401 gtatagtcgg cctctgatca cgcccttcat ggcccaggcc ctgtcgaagc ttggcgacgg
     8461 caacaccata ctgacagcga cgacgacttt caggaacgag ccgttcaatg cagccacgcc
     8521 ccttcccgct ccggccacgc catgatccgc gaaagtcttc cggcatttat cgtgcgtctg
     8581 gcgcgcgacc gtcgcggggt ctcggcggtc gagttcgccc tgatcgcgcc ggtgatgatc
     8641 gcattctact tcgcaatggg cgagttgtgc caaggctata tggcccagaa gcgcctttcc
     8701 cacacggctt cgtcgatcgc caacctcgtg gcgcagacgg acgtggtgaa agaggacgaa
     8761 gtggccgacg ttatggccat cggcgccctg atcatgaagc ctttcccggc cacctcgctg
     8821 tcgacacgaa taaccagcgt aacccgtgac gccaatggtg tcgcaaaggt cgactggagc
     8881 cgggccagcg gcgccggcct gcccgcgtat gccgaccagg ccgtcgtaac cgtgccgact
     8941 gacatgatcg gcaatggcgc gagcctcgta atggctgaag cca
//
LOCUS       DASVTM010000336         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_563401,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000336 DASVTM010000000
VERSION     DASVTM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563401"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..567
                     /locus_tag="VF633_09175"
     CDS             169..567
                     /locus_tag="VF633_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8471270.1"
                     /translation="MDLSIGELGRRTDTKVETVRYYERIGLLASPARTESNYRSYGQS
                     DLSRLSFIRRARDLGFPIDRVRALLDLADRRDHSCAEVDKMARSHLDEVERKIADLLA
                     LKQELGSLLAQCRGGTISECLIIEALGPKP"
     gene            complement(969..1541)
                     /locus_tag="VF633_09180"
     CDS             complement(969..1541)
                     /locus_tag="VF633_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX8471271.1"
                     /translation="MARDLPTESETREILARRRTRPAPRPAPPAGRALTGFIKDLDAK
                     FGRGAGALEPRWREIVGDQLARVTRPQKLTKGRAGTGGTLELRVAGPAALLVQHQSAE
                     ILARVNLFLGPGAVDKLRIAQGPVKPLAMPAASTKGARRRIDPLDAAAEAELTQSVAK
                     APDALKAALAGLGRAVLSDEAKQRAKTSRR"
BASE COUNT          273 a          496 c          567 g          296 t
ORIGIN      
        1 aacctcgaga acctgctgaa cgtgcgccag acacgctatg atcagctgtt gctgcggacg
       61 cgcgccaacg acggaagctg gaccgtggac ggctggggac cggcggaagg cttcaccgtc
      121 aatggcggtt tgaggatgca cttcggcggc ggtcattgag gagaaggcat ggacctgtcc
      181 attggcgaac tgggacggcg aaccgatacg aaggtcgaga cagtccgata ctatgagcgg
      241 atcggcctgc tggcctcacc agcccggacc gagagcaact atcgatccta cgggcagtcg
      301 gatctgagcc gcctgtcgtt catacgtcgg gcgcgtgacc ttgggtttcc tatcgaccgt
      361 gtccgagccc tgctggacct cgcagaccgt cgtgaccatt cctgcgctga ggtggacaag
      421 atggcccgct ctcacctcga tgaggtcgag cgcaagatcg ccgatcttct ggcgctgaag
      481 caggaactgg gttccctgct agcccagtgt cgaggcggca cgatttcgga atgtctcatc
      541 atcgaggctt tgggtccaaa accgtaagtc ggctatgggt gtttgccgac atccgctcac
      601 gccccagcag agattggatc aaacctccga gcctgcatcg gtccggccgc aaaacctttt
      661 gagatcaatc gtctgcatag ccgcgataca gatcggtcca gcagtggctg gcgcaggtgt
      721 agacttggcg tcttacggtc tttgacatgt ctatcgacgg gcggtgcggt atgcacttcg
      781 ccgcacgagg gtgcaaaggc gcccaaaaat cgacccagaa gataccgagc ctgttcagtg
      841 ccttagcgcc tcaagctcgg acgcagcttc cgaattggac gttttggact ctgttttttg
      901 gagtgcacat ccgggtgagg cagccgtctt gcaccggacc cggcggccgg aacgcgtcgc
      961 ggcccttctc aacggcgcga ggtctttgcg cgctgcttgg cctcgtcgga caacaccgcg
     1021 cggcccagac cggcgagggc ggccttgagg gcgtcggggg ccttggcgac ggactgggtc
     1081 agttcggctt cggcggcggc gtcgagcgga tcgatgcggc ggcgggcgcc cttggtggag
     1141 gcggcgggca tggccagcgg cttcaccggt ccctgcgcaa tgcgcagctt gtcgaccgcg
     1201 cccgggccga ggaacaggtt cacccgggcg aggatttcag ccgactgatg ctggaccagc
     1261 aaggcggcgg ggccggcgac gcgaagctcg agcgtgccgc cggttccggc gcggcccttg
     1321 gtcagtttct gggggcgggt gacgcgggcc agttgatcac cgacgatctc gcgccagcgc
     1381 ggttcgaggg cgccggcgcc gcgtccgaat ttcgcgtcca gatccttgat gaagccggtc
     1441 agggcgcgac cggccggagg cgccggtcgg ggcgcagggc gcgtgcgacg gcgggcgagg
     1501 atctcgcgcg tttcgctttc cgtgggcagg tcgcgggcca tacgcttata tagcgcgact
     1561 gatgatcgac gtcgccctcc acaaatccga cattcgggcc gcgctgctgg cctggtacga
     1621 cgccggggcg cg
//
LOCUS       DASVTM010000337         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_724964,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000337 DASVTM010000000
VERSION     DASVTM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724964"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF633_09185"
     CDS             complement(<1..839)
                     /locus_tag="VF633_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8471272.1"
                     /translation="MSAADFQIDESGGDSAVLRLSGDWTTVGLGQAAQRLSDELSGRK
                     VSSIDISDLGRFDTAGALIIAQATDKSLAADAWSSRPEAGRIYTMVDKLERESAPPPK
                     RPDTFTRTFSKIGHGVYDVGGEMILSMAFLGRLIAASGTALRHPGDIRWPAWFSQGER
                     AGLDAIPIVAVTNFFIGAVIAFLGANLLSQFGAGVFTVQLVAVATLRELAVLITAILL
                     AGRSASSFAAEIGSMRMNQEVDAMQVMGVNPFQALVIPRLAAMLMMMPLLTFIGILAG
                     LLGG"
     gene            complement(948..2393)
                     /locus_tag="VF633_09190"
     CDS             complement(948..2393)
                     /locus_tag="VF633_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268867.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX8471273.1"
                     /translation="MRVAALASEIDFEGWRLAARAFRLDGVTPGEARFVVAGSDGQEG
                     LFDAPTALSEPVRKEKTFAVPQAFVDMAKDVILHRTPDRFDLLYRILWRFRAEPDLMR
                     VLSDRDLSDAMDRQRNVSRASHKMKAFVRFRQAFDDDGECWVAWFEPAHRVLARTAPF
                     FVNRFTTMRWSLLTPDGSCFWDGKTLSFGPPADASDAPQEDEVEEFWKTYYASTFNPA
                     RLKTKTMQGEMPKRYWKNLPEASLIPELVAASAVRTEVMVAKPHTEPNARLAKAIFKH
                     NRDGAFDEGFSPSNLDELRHGVQSCRRCPLWRDATQGVCGEGPPGAKLMIVGEQPGDQ
                     EDLAGRPFVGPAGRVLNEALKEAGIDRDDAYVTNAVKHFKHEARGKRRLHRTPVTSEI
                     TACRWWLNHERALVKPKVVLTLGSTASFGVLGRKVAVTKERGMPVPLSDGSSAVITVH
                     PSYLLRIPDEESKRREHAAFIQDLKRVRQLL"
     gene            complement(2393..3634)
                     /locus_tag="VF633_09195"
     CDS             complement(2393..3634)
                     /locus_tag="VF633_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HEX8471274.1"
                     /translation="MAQLDLRGKLAVLADAAKYDASCSSSGTSKRNSVGGKGIGSTEG
                     MGICHAYTPDGRCVSLLKILLTNFCIYDCAYCINRVSSNVQRARFTVDEVVELTLNFY
                     KRNYIEGLFLSSGIIRSGDYTMEQLVLVAKKLRVDHDFRGYIHLKLIPEADPRLVAEA
                     GLYADRLSANIEVPREEALTRLAPQKSMAVIKKAMGQVRLAVEAAKPQKKDRAKPPKF
                     APGGQSTQLIVGADGAPDTEILDRSASLYGGYGLRRVYYSGFSPIPDSSAALPLSKPP
                     LMREHRLYQADWLMRFYGFSAPEIGEATTDGMLDLAVDPKLAWALNRREQFPVDVNTA
                     PREVLLRVPGLGVKSVDRMISVRRYRTLRLEDIGRLCRGIDKVRPFITALDWSPGGLT
                     DAVDLRARLTPTVQPVQLSLF"
     gene            complement(3724..>4280)
                     /locus_tag="VF633_09200"
     CDS             complement(3724..>4280)
                     /locus_tag="VF633_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX8471275.1"
                     /translation="HQGSYMFAGSNTSTAPVTVSSMAELAAAPSVVSTFTNDSLPPTS
                     RLGEATTVRTGYLADDIGTTIFQIFRDIQAYSNDPLTGPLTGKPNAAQKTFLTAQLSR
                     LDGASASTIDVTSRNGSLAKQVDSTNDSHKAQVAQLGEMVSAKTDADLARAVTDIQLA
                     QIAVQASAQVISQLKDTSLLNYLR"
BASE COUNT          785 a         1381 c         1331 g          783 t
ORIGIN      
        1 ccgcccaaaa gcccggcgag aatgccgatg aacgtcagca acggcatcat catcagcatg
       61 gcggccagac gcgggatgac cagggcctga aacggattga cccccataac ctgcatggcg
      121 tcgacttcct ggttcattcg catcgacccg atctcggccg cgaaggacga ggcggagcgg
      181 cccgccagaa ggatggccgt gatcaggaca gcaagctcgc gcagggtggc gaccgcgacc
      241 agttggacgg tgaagacgcc ggcgccgaac tggctcagca ggtttgcgcc aagaaaggcg
      301 atgactgcgc cgatgaagaa attcgtgact gcgacgatcg gaattgcatc cagacccgcg
      361 cgctcgccct gactgaacca ggcgggccag cggatgtccc cgggatgcct cagcgccgtg
      421 ccggaggctg cgatcagccg ccccagaaag gccatcgaca ggatcatctc gccgccgacg
      481 tcgtagacgc catgaccgat cttggaaaat gtgcgcgtga aggtgtcggg ccgtttgggc
      541 ggaggggcgc tttcgcgctc cagcttgtcg accatcgtgt aaatgcgacc cgcctcgggg
      601 cgcgaagacc aggcgtcggc tgccaacgac ttgtcggtcg cttgggcgat aattagagcg
      661 ccggcagtat cgaacctgcc cagatcggag atgtcgatgc tggaaacctt acgcccggac
      721 agttcgtcac tcaggcgttg cgccgcctga cctagaccta ccgtggtcca gtcaccgctg
      781 aggcgcagca ctgcgctgtc gccgcccgac tcgtcgatct gaaaatctgc tgcgctcatc
      841 ccctcccctt tagccgagcc gacgccaaac gcccatggcg aagctccgtc cgaggcctga
      901 caacgccgat taggtcaagt tgttcagttc accgtggcag ttacgcgtca caacagttgt
      961 cgaacgcgtt tcaggtcctg gataaacgcc gcgtgttctc gccgtttcga ctcctcatcc
     1021 ggaatgcgca gcaggtagga cgggtggacg gtgatcaccg cgctcgaacc gtccgacagg
     1081 gggaccggca tgccgcgctc cttggtcacc gcgaccttgc ggccaagcac gccgaacgat
     1141 gcggttgatc caagcgtcag cacgaccttg ggcttcacca gcgcccgctc gtgattcagc
     1201 caccagcggc aagcggtgat ttcactcgtc accggcgtac ggtgcagacg gcgtttgccc
     1261 cgcgcttcgt gcttgaaatg tttgacggcg ttggtgacgt aggcgtcgtc gcggtcgatg
     1321 ccggcctcct tgagggcctc gttcagcacc cttccggcag gtccgacgaa gggacgacca
     1381 gccagatcct cctgatcgcc cggctgctca cccacgatca tcagcttcgc gcccggtgga
     1441 ccttcgccac aaacgccttg ggtggcatca cgccagagcg gacagcgacg gcaggactgc
     1501 acaccgtggc gcaactcgtc gagattcgat ggcgaaaacc cttcgtcgaa cgcgccgtcg
     1561 cgattgtgct tgaagatggc cttggccagt cgggcgttgg gttcggtgtg cggtttggcg
     1621 accatgactt cggttcgaac tgccgaggcc gcgaccagtt ccgggatcag cgatgcctct
     1681 ggcaggttct tccagtaccg cttgggcatc tcaccctgca tggtcttcgt cttcagccgg
     1741 gccgggttga aggtggaggc gtaataggtc ttccagaact cctcgacctc gtcctcctgt
     1801 ggcgcgtcgg aggcgtcggc gggcgggccg aacgacaggg tcttgccgtc ccagaagcag
     1861 gatccgtccg gcgtcagcag cgaccagcgc atcgtagtga accggttgac gaagaacgga
     1921 gcggtgcgcg ccagcacccg gtgcgcgggc tcgaaccacg ccacccagca ttcgccgtca
     1981 tcgtcgaagg cctgccgaaa gcggacgaag gccttcatct tgtggctggc ccggctgacg
     2041 ttacgctgtc ggtccatggc gtcggacaag tcgcggtccg acagcacccg catcaggtcc
     2101 ggctcggccc ggaagcgcca caggatgcgg tacagcaggt cgaaccggtc aggcgtccgg
     2161 tgcaggatga cgtccttggc catgtcgacg aaggcttgtg ggaccgcgaa ggtcttttcc
     2221 ttgcgcacag gctcgctcaa ggcggtgggt gcgtcgaaca acccctcttg cccgtccgaa
     2281 ccggccacca cgaaccgcgc ctcgcccggc gtcacgccgt ccagccggaa agcccgagcc
     2341 gccagccgcc agccctcgaa atcgatctcc gacgccaggg ccgcgacccg catcagaaca
     2401 ggctcagctg caccggttgg accgtcggcg tcagccgcgc ccgcagatcg actgcgtccg
     2461 tcagcccgcc gggtgaccag tccaacgccg tgatgaaggg ccgcaccttg tcgatgcccc
     2521 ggcacaagcg accgatatcc tcgagcctga gcgtgcgata gcggcggacg ctgatcatgc
     2581 gatcgaccga ctttaccccc agccccggca cgcgcagcag gacctcacgc ggggcggtgt
     2641 tcacatcgac cggaaactgc tcgcgccgat tgagcgccca agccagcttg ggatcgacag
     2701 cgaggtccag catcccgtcc gtcgtcgcct caccgatttc gggggccgag aagccataga
     2761 aacgcatcag ccagtccgcc tgatacagac ggtgctcgcg catcagcggc ggcttcgaca
     2821 acggcagagc cgcgctggaa tccgggatcg gactgaagcc cgaataatag acccgacgca
     2881 ggccataacc gccgtacagc gaggcgctgc ggtcgaggat ttcggtgtcc ggcgcgccgt
     2941 cggcgccgac gatcaactgc gtgctctggc cgccgggcgc gaacttcggc ggcttggcgc
     3001 ggtccttctt ctggggtttg gccgcctcga cggcgagccg cacctgcccc atcgccttct
     3061 tgatcacggc catgctcttt tgcggcgcca gccgggtcag cgcctcttcg cgcggcacct
     3121 cgatattggc ggacagccgg tcggcataga gaccagcctc tgcgaccaac cttggatcgg
     3181 cttcggggat cagcttgaga tggatgtaac cgcggaaatc gtggtcgacc cgcagcttct
     3241 tggccaccag caccagctgt tccatcgtgt agtcacccga gcgtatgatg cccgacgaca
     3301 ggaacagccc ctcgatgtag ttgcgcttgt agaaattgag cgtcagctcg accacctcgt
     3361 cgaccgtaaa ccgcgcccgc tgcacgttcg aggacacccg gttgatgcag taggcgcaat
     3421 cgtagatgca gaaattcgtc agcagtatct tgagcaggct gacgcaccga ccatccggcg
     3481 tataggcgtg acagatgccc atcccctcgg tcgagccgat gcccttgccg ccgacggagt
     3541 tgcgtttgga cgttcctgac gacgaacagg aggcgtcgta tttcgccgca tccgccaaga
     3601 ccgccagttt tccgcgtaga tcgagctgag ccatgttccc attatgttct caacaagaac
     3661 cagggtccag cgtggccata atatccagcg gagataatcg cttattgtcc ggcttcggcg
     3721 cctttatcgc agatagttga gcagcgacgt gtccttgagc tggctgatta cctgtgccga
     3781 agcctggacg gcgatctgag ccaactggat gtcggtcacc gcccgcgcca gatcagcgtc
     3841 ggtcttggcg ctgaccattt ccccgagctg ggcaacctgg gccttgtggc tatcgttagt
     3901 ggaatcgact tgtttggcca gtgatccgtt tcgtgaagtg acatcgatcg tgctcgcgct
     3961 cgccccatcc agtcgactca gctgagcagt caggaaggtc ttctgcgcgg cgtttggctt
     4021 gcccgtaaga ggtcctgtca ggggatcatt gctgtaggcc tgaatatccc tgaatatctg
     4081 gaagattgtc gttcctatat cgtcggccag atagccggtg cgaaccgtcg tggcctcgcc
     4141 gaggcgcgat gttggcggaa gactgtcgtt ggtgaacgtc gagactacgc taggcgcagc
     4201 agcgagttcg gccatgctcg acaccgttac cggcgccgtg ctcgtgttgg aaccggcaaa
     4261 catataactt ccctgatgct
//
LOCUS       DASVTM010000338         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_349930,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000338 DASVTM010000000
VERSION     DASVTM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349930"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VF633_09205"
     CDS             <1..285
                     /locus_tag="VF633_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX8471276.1"
                     /translation="VVEKHDFGDGRSRYEEAGDDHHDHLIDTKTGEVIEFFDAEIERL
                     KTEIAERLGFELVGHKLELYGKAIEGAEPSKREGLMFKRHAARVDPETLS"
     gene            349..1788
                     /gene="miaB"
                     /locus_tag="VF633_09210"
     CDS             349..1788
                     /gene="miaB"
                     /locus_tag="VF633_09210"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX8471277.1"
                     /translation="MSETLVHLEPDASPPVGAPKVAAPAKRLFIKTYGCQMNVYDSER
                     MADVLRPLGYATTDTAEGADFVILNTCHIREKAAEKVYSELGKLREMKLEKAAAGLGS
                     MTIAVAGCVAQAEGEEIMRRQPAVDLVVGPQAYHQLPELLTRTARARGERIGADFAPN
                     EKFDALASGGAVRGVNGPTAFLTVQEGCDKFCTFCVVPYTRGAEWSRPVADVLAEARG
                     LAAQGVREVTLLGQNVNAYDGVSDGVGESGVWSLAKLTKALGEIPGLDRIRYMTSHPN
                     DMADDLIAAHAEGADGVAALMPYLHLPVQSGSDRILRLMNRKHGRQKYFDLIDRVREA
                     RPDIALSGDFIVGFPGETDKDFADTMDLVRRIGYASAFSFMYSPRPGTPASGMGAQVP
                     DAVARERLHALQALLFEQQAAFNAAQTGKTLDVLFEKKGRHGRQAIGRSPYLQSVHVE
                     DADHLIGEIVQVEIIVGQQNSLKGRLLGA"
     gene            1778..2782
                     /locus_tag="VF633_09215"
     CDS             1778..2782
                     /locus_tag="VF633_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX8471278.1"
                     /translation="MARESEFLALDDDALHAVVGPNSRHVALIEDAFKVLIEAPGGGV
                     SINGGARDRANAKQAIEALAARVAKRAEVDEADVRAAIGSARTGVTGGGRRVDPMALP
                     IGKRGAIVPKTDAQARYLEVLANHELSFGVGPAGTGKTFLAAAYGASLLRRGQVDRLV
                     ITRPAVEAGEKLGFLPGDLNEKVDPYLAPIWEALNDILGAEDVQRRRDKGEIEAAPIA
                     FMRGRTLSHAFVIVDEAQNTSRLQMKMVLTRLGEGARMVVTGDPSQIDLLNPRDSGLA
                     HALRILQGVKGVGVLQFEAQDVVRHAMVERIVRAYDADAANSGPGGSRPKADLEDTPR
                     "
     gene            2796..>2988
                     /locus_tag="VF633_09220"
     CDS             2796..>2988
                     /locus_tag="VF633_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HEX8471279.1"
                     /translation="MIEVEIESDDWTNALSDAEAVVERAASAALADVAGDLVVLLTDN
                     GAVQLLNAEFRDKDKPTNVL"
BASE COUNT          554 a          963 c          971 g          500 t
ORIGIN      
        1 gtggtcgaga agcacgattt cggagatggc cgcagccgct acgaagaggc tggcgacgat
       61 catcacgatc atctgattga taccaaaacc ggcgaagtca tcgaattctt cgacgctgaa
      121 atcgagcggc tgaagactga aatcgccgaa cgcctgggct tcgaactcgt gggtcacaag
      181 ctcgagcttt acggcaaggc cattgagggc gccgaaccat cgaagcgtga aggcctgatg
      241 ttcaagcgtc acgccgctcg cgtcgatccg gaaacgctgt cctgatcctg atacgcacgc
      301 gcgctcctga agtggcgcga acgtccagag cggactagat acgctcccat gagcgaaacc
      361 ctggtccatc tcgaaccgga cgcatcccct cccgtgggcg cgcccaaggt cgctgcgccc
      421 gcgaagcgcc tgttcatcaa gacctacggc tgtcagatga acgtctatga cagcgagcgc
      481 atggctgacg tcctgcgccc gcttggttac gccacgaccg acacggccga gggcgccgac
      541 ttcgttatcc tgaacacctg tcacatccgc gagaaggcgg ccgagaaagt ttattccgaa
      601 ctcggcaaac ttcgggaaat gaagctcgag aaggccgcag ccggactggg gtcgatgacc
      661 atcgccgttg ccggctgcgt cgcccaggcc gagggcgaag agatcatgcg ccgtcagcct
      721 gcggtcgatc tggtcgtcgg gccacaggcc tatcaccagc tccctgagct tctgacccgc
      781 accgcccggg cgcgcggcga acggatcggc gccgatttcg cccccaacga aaagttcgat
      841 gcgcttgcgt cgggcggcgc ggtcagaggc gtcaacggcc ccacggcctt cctgacagtg
      901 caggaaggct gcgacaagtt ctgcaccttc tgcgtcgtgc cctatacccg cggcgcggaa
      961 tggtcacgcc cggtcgccga cgtactggcc gaggcgcgcg gactggcggc gcagggagtg
     1021 cgtgaggtta ccctgctggg ccagaacgtg aatgcctatg acggcgtctc tgacggtgtt
     1081 ggcgaaagtg gcgtctggtc gctggcgaag ttgacgaagg ctctgggcga aattcccggc
     1141 ctcgatcgca tccggtacat gaccagtcat cccaacgaca tggccgacga cctgatcgca
     1201 gcccatgccg aaggcgcgga cggcgttgcg gctctgatgc cctatctgca tctgccggtg
     1261 cagtcggggt ctgaccggat cttgcggctg atgaaccgca agcacggtcg ccagaagtat
     1321 ttcgacctga tcgaccgggt ccgcgaggcg cgtccggaca tcgccctgtc gggtgatttc
     1381 atcgtcggtt tccccggcga gacggacaag gatttcgcgg acaccatgga tctggtccgc
     1441 cggatcggat acgcgtcggc cttctccttc atgtattcgc cgcgccccgg cacgcccgcc
     1501 tccggcatgg gagcgcaggt gcctgatgcc gtggcgcgcg aacggctgca tgcccttcag
     1561 gcccttttgt tcgaacaaca ggcggcgttc aacgcggcgc agacaggcaa gaccctggac
     1621 gtcctgttcg agaaaaaggg ccgccatgga cgccaggcga tcggccgttc gccttatctg
     1681 caatcggtcc atgtcgaaga cgcggatcat ctgatcggcg agatcgttca ggtggagatc
     1741 atcgttggtc agcaaaacag cctgaaaggc agacttcttg gcgcgtgaga gtgaattcct
     1801 ggccctcgac gacgacgccc tgcatgcggt cgtcggccca aacagccgcc acgtcgctct
     1861 gatcgaggac gccttcaagg tgctgatcga ggcgcccggc ggcggcgtgt cgatcaatgg
     1921 cggcgcccgc gaccgggcta acgccaaaca ggccattgaa gccttggcgg cccgggtcgc
     1981 caagcgcgcc gaggtcgatg aggccgacgt ccgcgccgcc atcggctcgg cccgcacagg
     2041 cgtcaccggc ggcggccgca gggtcgatcc catggccctg ccgatcggca agcgtggcgc
     2101 catcgtgccc aagaccgacg ctcaggcgcg atacctcgag gtgctggcca atcacgaact
     2161 cagcttcggc gtcggccctg caggcactgg gaagaccttt ctcgctgcgg cttacggcgc
     2221 gtcgctgctg cgacgcggac aggtcgaccg gctggtcatc acccgcccgg cggtcgaggc
     2281 gggtgaaaag ctcggcttcc tgcccggcga cctgaacgag aaggtcgatc cctatctggc
     2341 cccgatctgg gaagccctga acgacattct cggggccgag gacgtccagc gccgtcgcga
     2401 caagggcgag atcgaagccg cccccatcgc cttcatgcgc ggccgcacgc tgagccacgc
     2461 cttcgtgatc gtcgacgagg cgcagaacac atcgcggctg cagatgaaga tggtcctgac
     2521 ccgcctgggg gaaggggcgc gcatggtggt gaccggtgac ccgtcgcaga tcgacctgct
     2581 caatccgcgt gactctggcc tcgcccatgc gctgcgtatc ctgcaagggg tgaaaggcgt
     2641 cggcgtgctg cagtttgaag cccaggacgt cgtgcgccac gccatggtcg agcggatcgt
     2701 gcgcgcctac gacgcggatg ctgccaattc tggaccggga ggaagccgcc ccaaagctga
     2761 tctcgaagac actccccggt gatcggaata gcctcgtgat cgaggtcgag atcgagtccg
     2821 atgattggac aaacgccctg tccgacgccg aggccgtggt cgaacgcgcg gcgagcgcag
     2881 cgctggcgga tgtcgcgggc gatctggttg tgctgctgac cgacaatggc gccgtgcaac
     2941 tgctgaacgc cgagttccgc gacaaggaca agccaaccaa cgtcctct
//
LOCUS       DASVTM010000339         6539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_245592,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000339 DASVTM010000000
VERSION     DASVTM010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245592"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VF633_09225"
     CDS             complement(<1..404)
                     /locus_tag="VF633_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471280.1"
                     /translation="MTRKLSAITAVAAILAAVPFAASAQQGGPMGGMAQMGPFTLAQM
                     QERSDTRFTALDANHDGVLSTAEATPPPTPGSMMSAGRGMERADADHDGNVTLEEYHA
                     MTAGMFARMDANHDGTVTPEELQAMRGMMRGGG"
     gene            531..1496
                     /locus_tag="VF633_09230"
     CDS             531..1496
                     /locus_tag="VF633_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8471281.1"
                     /translation="MALSADQIIDALRAAGEPTRLRVLALLTGEELSVMELSRVLQQS
                     QPRVSRHLKLMTEAGLIERFPDGARVFYRISSDLPARRLVETVLDLLEDTEGESDHQR
                     LDEVRGERAAAAAAYFEQVAPQWDRVRSLYVAETTVEKAVEEAAGPGPFERVVDLGTG
                     SGRMLTLLGKKAKMSVGLDLSHNMLNIARSNVTRAGIDKVELRHGDIFATRLPGASAD
                     LVIVHQVLHYLADPHAAVIEAARLVSPGGKLLIVDFAPHEMESLREEHQHRRLGFTDE
                     EISSRLTDCGLDVRPTVALTPDADGLTVTIWTGVRAASSDFRKAA"
     gene            1496..2482
                     /gene="metF"
                     /locus_tag="VF633_09235"
     CDS             1496..2482
                     /gene="metF"
                     /locus_tag="VF633_09235"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX8471282.1"
                     /translation="MASSVAQTTVQSPAQPQGLAAARALLLSPLGPVARAGDNANSVR
                     VSFEFFPPKTDKAEETLWEAVRRLEPLNPEFVSVTYGAGGSTRERTHRTVQRMLTETT
                     LKPAAHLTCVEATKAEVDEVIESYKAIGVDHIVALRGDPPGASGIGGVYQPRADGYAN
                     ATELSQAISRVGGFDITVGVYPEKHPESPSIDHDIDVIKAKIDAGATRLISQFFFDID
                     AFLRFRDRIRAAGITAPLLPGIMPVSNFGALQRMSASCGASVPQWLATHFDGLDDDPE
                     TRKLLAASVAAETCARLQEEGFSDFHFYTLNRADLVYAICRVLGVREQKAVA"
     gene            2483..3577
                     /locus_tag="VF633_09240"
     CDS             2483..3577
                     /locus_tag="VF633_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX8471283.1"
                     /translation="MTSPLTRPERIAALHKAAKERILVLDGSWGVMIQRRGLEEADFR
                     GDRFVEANGYNERDQMKGNNDILCITRPDVISDLHDQYFGAGADISETNTFSGTIIAQ
                     DDYKLDEQAVWDINLEGAKIARAAADRWTEKEPHKPRFAAGSIGPLNKMLSMSSDVND
                     PGARLVTFDQVYEAYRHQIKALNEGGVDLYLIETITDTLNCKACIKAIKDLEDEGMDA
                     LPIWISGTITDRSGRTLSGQTAEAFWNSVRHAKPFAIGFNCALGADLMRPFIAELSRV
                     ADTLVAAYPNAGLPNAMGQYDEEPHETAHSIEEWAASGLVNIVGGCCGTTPDHIRHTA
                     EAVSKLPTREMPERPVAMRLSGLEPFELVA"
     gene            3577..6243
                     /gene="metH"
                     /locus_tag="VF633_09245"
     CDS             3577..6243
                     /gene="metH"
                     /locus_tag="VF633_09245"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX8471284.1"
                     /translation="MRPVFINVGERTNVTGSAKFKKLIIEGDYSAALTVARQQVEAGA
                     QVIDINMDEGLLDSKQAMITFLNLIAAEPDIARVPVMIDSSKWEVIEAGLKCVQGKPI
                     VNSISMKEGEDAFRAYAVKCMRYGAAIVVMAFDEVGQADTAARKIEICTRAYRILVDE
                     VGFPPEDIIFDPNIFAVATGIEEHDNYAVDFIEAVKVIKATLPYARISGGVSNVSFSF
                     RGNEPVRRAIHSVFLYHAIAAGMDMGIVNAGDLPVYTDIEPTLREAVEDVILNRPQRT
                     NVSNTERLVDIAPNYKGDKGVARVVDLKWRDQPVGKRIEHALVNGITEYIDADTEEAR
                     LSFDRPLHVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAWLNPFMEAEKA
                     GKPREQAGRILMATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVMVPADRILDAAIEHN
                     VDIVGLSGLITPSLDEMVFVAREMQRRGFDIPLLIGGATTSRTHTAVKIEPGYVAGST
                     TYVVDASRAVGVVSGLLSKTERAKNEAITRDEYIRIREQYARGQEVKARASLVQARAN
                     RFKPDPDAAMPGKPAFIGVRAFESWDLQDLADHIDWTPFFASWELIGRYPLILEDEIV
                     GQAAQDLFEDAQIMLKQIVAEKWFTAKGVVGFWPARADGDDVVVFSDESRAEEIARFH
                     TLRQQIKKSNGKPNLALSDFIAEEGDDYIGAFAVTAGHGELEIAKKFKDAGDDYSSIM
                     AAALADRLAEAFAERLHKEVRTQLWGYAADEVSTPDELIAEQYQGIRPAPGYPAQPDH
                     TEKATLFRLLQAETNAGMALTESYAMTPPASVSGLYFGHPGSHYFGVGKIDLDQIEDY
                     ARRKGWDVETAERWLSPILNYEPGQVARTVAA"
     gene            complement(6283..>6539)
                     /locus_tag="VF633_09250"
     CDS             complement(6283..>6539)
                     /locus_tag="VF633_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471285.1"
                     /translation="AAAGPRQSAATRAAATAAAAEATARWTPVLARTDEASRSQSFGL
                     FFGLPGRCEHAARRIRANIATPPATLADVGLRSEGVDQAQ"
BASE COUNT         1218 a         2223 c         2000 g         1098 t
ORIGIN      
        1 ccaccaccgc gcatcatccc gcgcatggcc tgaagttctt caggcgtcac cgtgccgtcg
       61 tggttggcgt ccatgcgagc gaacatcccc gccgtcatgg cgtggtattc ctcaagagtg
      121 acattgccgt cgtggtcggc gtccgcgcgt tccatgccgc gaccggcgct catcatgctg
      181 cctggagtcg gcggcggcgt cgcctctgcc gtggacagga cgccgtcatg gttggcgtcg
      241 agcgcggtga aacgggtatc cgaacgctcc tgcatctgcg ccagggtgaa cgggcccatc
      301 tgggccatcc cgcccatggg gccaccctgc tgggcggagg cggcgaaagg gacggccgcg
      361 agaattgcgg cgacagcagt gatggcggac agtttgcggg tcatggaatt ctcctgaaga
      421 ctcccgttca acggtcgttt cgaaccaatc cttcgcgcca tttccacgga aagtgcgagc
      481 gccgcagttc tttcttgcaa aatcatataa ggatatcctt atatgtgcgg atggcattgt
      541 ccgcagatca gatcatcgat gcccttcgcg ccgctggcga gccaacgcgt ttgcgggtgc
      601 tggcgctgct tacgggggaa gaactctcgg tcatggagtt gtcccgtgtc ctgcagcaaa
      661 gccagccccg ggtctcacga catctgaagc tgatgaccga ggcagggttg atcgagcgct
      721 tccctgacgg cgcgcgggtc ttctaccgca tatcgtccga cctccccgca cgtcgcctcg
      781 tggagacagt gctggacctg ctggaagaca ccgagggtga aagcgatcac cagcgtctcg
      841 acgaggtgcg cggtgagcgc gccgccgccg ccgccgccta tttcgaacag gtagcgccac
      901 agtgggaccg cgtccgctcc ctctacgtcg ccgagacgac cgtcgagaaa gctgtcgagg
      961 aggccgccgg tcccggcccg ttcgagcgcg tcgtggatct gggcaccggg tcgggtcgga
     1021 tgctgacgct gttgggcaag aaggcgaaga tgtcggtcgg gctggatctc agccacaaca
     1081 tgctcaacat cgcccgcagc aacgtcaccc gggcgggtat cgacaaggtc gagctcaggc
     1141 acggcgatat tttcgcaacc cggctgccgg gcgccagcgc agatctcgta atcgtgcatc
     1201 aggtcctgca ttatctggcc gatccccacg cggcggtcat agaggctgcg cggctcgtct
     1261 cgccgggcgg aaagcttctg atcgtggact tcgctcccca tgagatggag tctcttcgcg
     1321 aggagcacca gcaccgccgc ctgggcttca ccgacgaaga gatatccagc cggctgaccg
     1381 attgcggcct cgatgtccgc ccaaccgtag cgctgacgcc tgacgccgac ggcctgaccg
     1441 taacaatctg gaccggcgtc cgcgccgcct cctcagattt ccgaaaggcc gcttgatggc
     1501 tagctccgtc gcccaaacca ccgttcaaag cccagcccag cctcagggtc tggctgcggc
     1561 ccgggctctt ctgctatcgc ctttaggtcc ggtcgcccgc gccggtgaca acgccaattc
     1621 cgttcgtgtc tcgttcgagt tcttcccgcc caagaccgac aaggccgaag agactctgtg
     1681 ggaggctgta cgtcgcctcg agccgttgaa tcccgagttc gtctcggtga cctacggcgc
     1741 aggcggctcg acccgcgaac gcacccaccg caccgtccag cgcatgctga cggagaccac
     1801 gctcaagcct gcggcccacc tgacctgcgt cgaggccacc aaggccgagg tcgatgaggt
     1861 gatcgagagc tacaaggcca ttggcgtcga tcacatcgtg gccctgcgcg gcgatcctcc
     1921 cggtgcctct ggcatcggcg gcgtctacca gccccgtgcc gacggctacg ccaacgccac
     1981 tgagctgtcg caggcgatca gccgcgtcgg cggcttcgac atcacggtcg gcgtctatcc
     2041 ggaaaagcat cccgagagcc catccatcga tcacgacatc gacgtcatca aagccaagat
     2101 cgacgccggc gccacgcgtc tgatcagcca gttcttcttc gacatcgacg ccttcctgcg
     2161 gttccgcgac cggatccgcg cggcggggat caccgcgccc ctgctgcccg ggatcatgcc
     2221 ggtgtccaat ttcggcgccc tgcaaagaat gagcgcgtcg tgcggcgcat ccgtgccgca
     2281 atggctcgcc acccatttcg acgggctgga cgatgatccc gagacccgca agctgctggc
     2341 cgcttcggtc gccgccgaga cctgcgcccg cctgcaggaa gagggtttct ccgatttcca
     2401 cttctacacc ctgaaccgcg ctgacctcgt ttatgcgatc tgccgcgtgc tgggcgttcg
     2461 tgaacaaaag gctgttgcgt aaatgacgtc cccactcacc cgccctgagc gcatcgccgc
     2521 tctgcacaag gccgccaagg aacgcatcct cgtccttgac ggatcgtggg gcgttatgat
     2581 ccagcgtcgt ggtctggaag aagccgactt ccgcggcgac cgcttcgtcg aggccaacgg
     2641 ctataacgaa cgcgaccaga tgaagggcaa caacgacatc ctttgcatca cccgcccgga
     2701 cgtcatcagc gacctgcacg accagtattt cggcgccggg gccgacatct ccgagaccaa
     2761 caccttctcg ggcaccatca tcgcccagga cgattacaag ctggacgaac aggcggtctg
     2821 ggacatcaat cttgaaggcg cgaagatcgc ccgcgccgcc gccgaccgct ggacggaaaa
     2881 ggaaccgcac aagccgcgct tcgccgccgg ttcgatcggg ccgctcaaca agatgctgtc
     2941 gatgtcgtcg gatgtgaacg atcccggcgc gcgtctggtc accttcgatc aggtctacga
     3001 agcctaccgc catcagatca aggcgctgaa cgaaggcggc gtcgacctgt atctgatcga
     3061 gaccatcacc gacacgctca actgcaaggc ttgcatcaag gccatcaagg acctcgaaga
     3121 cgagggcatg gacgccctgc cgatctggat ttcgggcacc atcaccgacc gctcgggccg
     3181 gaccctttcg ggccagacgg ccgaggcttt ctggaacagc gtgcgccacg ccaagccgtt
     3241 cgccatcggc ttcaactgcg cgctaggcgc cgatctgatg cggccgttca tcgccgaact
     3301 cagccgcgtc gccgacaccc tcgttgccgc ctatcccaac gccggtctgc caaacgccat
     3361 gggccagtac gacgaggagc cccacgaaac cgcccactcc attgaggaat gggctgcgtc
     3421 agggctcgtc aacatcgtcg gcggctgctg cggcaccacg cccgaccaca tccgccatac
     3481 tgcggaggcg gtctcgaaac tgccgacgcg cgaaatgccc gaacggccag tcgccatgcg
     3541 cctgtcgggc ctcgaacctt ttgagcttgt agcctgatgc gtcctgtatt catcaacgtc
     3601 ggcgagcgga ccaacgtcac cggctcggcc aaattcaaga agctgatcat cgagggcgac
     3661 tattccgccg ccctgaccgt ggcgcgccaa caagtcgagg ccggggcgca ggtcatcgac
     3721 atcaacatgg acgaaggcct gctcgattcg aagcaggcga tgatcacctt cctcaacctg
     3781 atcgcggccg agcccgacat cgcccgcgtg ccggtgatga tcgacagctc caaatgggag
     3841 gtgatcgagg cgggcctgaa atgcgtccag ggcaagccga tcgtcaactc gatctcgatg
     3901 aaggagggcg aggacgcctt ccgggcctat gcggtcaaat gcatgcgcta tggcgccgcg
     3961 atcgtggtca tggccttcga cgaagtgggg caggccgaca ccgccgcccg caagatcgag
     4021 atctgcaccc gcgcctatcg catcctcgtc gatgaggtcg gcttcccgcc cgaggacatc
     4081 atcttcgacc ccaacatctt cgccgtggcg acggggatcg aggaacacga caactacgcc
     4141 gtcgacttca tcgaggcggt caaggtcatc aaggccaccc tgccttacgc ccgtatctcg
     4201 ggcggggtgt cgaacgtgtc gttcagcttc cgcggcaacg agcccgtgcg ccgggcgatc
     4261 cactcggtct tcctgtacca cgccatcgcc gccggcatgg acatgggcat cgtcaacgcc
     4321 ggcgacctgc cggtctatac cgacatcgag ccgaccctgc gcgaggccgt cgaggacgtg
     4381 atcctcaacc ggccccagcg gacaaatgtc tccaacaccg aacgcctcgt cgacatcgcc
     4441 ccaaactaca agggcgacaa gggtgtggcc cgggtcgtcg atctgaaatg gcgcgaccag
     4501 ccggtcggca agcgcatcga gcacgcgctg gtcaacggca tcaccgaata catcgacgcc
     4561 gacaccgagg aagcccgcct gtcgttcgac cggccgctgc acgtcatcga ggggccgttg
     4621 atggacggca tgaacgtggt cggcgacctg ttcggctcgg gcaagatgtt cctgccgcag
     4681 gtggtcaaat ccgcccgggt gatgaagcaa gccgtcgcct ggctgaaccc gttcatggaa
     4741 gccgaaaaag ccggcaagcc gcgcgaacaa gccggccgca tcctgatggc caccgtcaag
     4801 ggcgacgtcc acgacatcgg caagaacatc gtcggcgtcg tgcttcagtg taacaactac
     4861 gaggtcatcg atctcggcgt catggtcccg gccgaccgga tcctcgacgc cgcgatcgag
     4921 cacaatgttg atatcgtcgg cctgtccggt ctgatcacgc cgtcgctcga cgagatggtg
     4981 ttcgtggcgc gcgagatgca gcgtcgcggc ttcgacattc ccctgctgat cggcggcgcc
     5041 accaccagcc gcacccatac tgcggtgaag atcgagcccg gctatgtggc cggttcgacc
     5101 acctatgtcg tcgacgcgtc acgcgccgtc ggcgtggtct caggcctgtt gtcgaagacc
     5161 gagagggcca agaacgaggc cattacccgc gacgagtaca tccgcatccg cgagcagtac
     5221 gcccggggtc aagaggtcaa ggcgcgcgcc tcgctggttc aggcccgggc gaaccgattc
     5281 aagcccgatc ccgacgccgc catgccaggc aagcccgcgt ttatcggcgt gcgggctttc
     5341 gaaagctggg atctgcagga tctggccgac cacatcgact ggacgccgtt cttcgccagc
     5401 tgggagctga tcggccgtta tccgctgatc ctcgaggatg agatcgtcgg tcaggcggcc
     5461 caggatctgt tcgaggacgc ccagatcatg ctgaagcaga tcgtcgccga gaaatggttc
     5521 acggccaagg gcgtggtcgg cttctggccc gcccgcgctg acggcgacga cgtcgtggta
     5581 ttctccgacg agagccgcgc cgaagagatc gcccgcttcc acaccctgcg ccagcagatc
     5641 aagaagtcga atggcaagcc caacctggcc ctgtcggatt tcatcgctga agaaggcgac
     5701 gactacatcg gcgccttcgc cgtcaccgct ggtcatggcg agctcgagat cgccaaaaag
     5761 ttcaaggacg ccggagacga ctattcgtcg atcatggctg cggccctcgc cgaccgtttg
     5821 gccgaggcct tcgccgaacg cctgcacaag gaggtccgca cccagctgtg gggctacgcc
     5881 gccgacgagg tgtcgacacc cgacgaactg atcgccgagc aatatcaggg catccgcccg
     5941 gcccccggct atccggccca acccgaccac acggaaaagg ccaccctgtt ccgcctgctg
     6001 caggccgaaa cgaacgccgg gatggccctg accgagagct acgccatgac gccgcccgcc
     6061 tcggtttcgg gtctgtattt cggccatccg ggcagccact atttcggtgt cggcaagatc
     6121 gatctggacc agatcgagga ctacgcccgc cgcaagggtt gggacgtcga gaccgccgaa
     6181 cgctggctgt ccccgatcct gaattacgaa ccgggccagg tcgcacggac ggtcgcggcg
     6241 taaagcttat cctcctcccc ctgcgggaga aggggaaggt gcctactgcg cctggtccac
     6301 gccttccgac cgcaagccca catcagccaa cgtcgccggc ggtgtcgcaa tattcgcgcg
     6361 aatacgccga gcggcatgtt cgcaccggcc gggcagaccg aagaacaacc cgaacgactg
     6421 gctgcgcgac gcctcgtcgg tccgggccag aaccggcgtc cagcgcgctg tcgcctcggc
     6481 cgctgcagcg gtcgctgcag cccgagtcgc ggcagactgg cggggcccgg ccgcagcca
//
LOCUS       DASVTM010000340         3835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_563885,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000340 DASVTM010000000
VERSION     DASVTM010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563885"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF633_09255"
     CDS             <1..328
                     /locus_tag="VF633_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8471286.1"
                     /translation="SRPTEVTGPEDPLAALSRRYADHIKTLSAEIDRLSEQSFEQRAE
                     GLANLAHAVAGTAGTLGFPSLSEAAFELEDCARQVISGDVDGTALEPAVQAFRDALNV
                     EAASRL"
     gene            530..955
                     /locus_tag="VF633_09260"
     CDS             530..955
                     /locus_tag="VF633_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471287.1"
                     /translation="MSAHVLIADDDPFLRELLAHKLSAAGYSVSSARHGGEALEQCRS
                     SLPDLIVLDAMMPVTDGFEVLRRLKSDPLTAAVPVIMLTAMKREEDVVGALKLGAADY
                     LVKPFIPDELVARIGRLLPTGTDHANTPDDRGSGLSRAN"
     gene            906..1916
                     /locus_tag="VF633_09265"
     CDS             906..1916
                     /locus_tag="VF633_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8471288.1"
                     /translation="MPTPLTIAVLACLAPIETARAQDQAGGSERVYEAAVEARLMGRT
                     TEAIEGSRRVLAVRPNDVDARLNLALALIAAGRLDEAEVELVTVLNQAPAYEDARVAL
                     DSIHRLRRDPVRWRLDVSASYSDLSQNLPPWRESALTISRRTIHGSVSGTIEHAERFR
                     RQDTYFEGRLDRSLGRGSVYGAVGGTPNADFRPEIAVRGGGQAPVFGQNLSATLDVGV
                     ARYAVGVVSTVQPGVEYTTSGGALTVGARWIHLWDERDTYRSGYSLRALLAIRPSVRV
                     RAGFADAPESSDGLTVDVRAASLGAEVDVNDRLTLRLNGLKEDRAAYDRDEITLGVGV
                     RF"
     gene            1919..2968
                     /locus_tag="VF633_09270"
     CDS             1919..2968
                     /locus_tag="VF633_09270"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8471289.1"
                     /translation="MNPLMLIWWTSLVLAGIAFSWMMGLIVARLFRENSEARRIADQQ
                     TLSAAYLMIMSGAGEAGTLLEPFRKRSRLLAESLLEVLSLVRGVERERLIASLIDAGV
                     ADRLRSRLTRGSRTGRLAAAEALSAFPSMATRTALRALHRRSTDTEIRIASVRTLIDI
                     GDPPNATELVADLEGKGYSESLLYAPVLRRLAVDAPDDALETLTLPGLGMAARAVMIE
                     AVGASGDYRALPALSLMAAGPEPGLRMSALRALGALAHPGSAETIVGALGDEAWDVRA
                     EAAMAAGRIGGPALVQALTPLLDDPVWWVRFRAAESMSKMGGSGIEALRYAATSQVDV
                     TRRSASLALAEQGLA"
     gene            2979..>3835
                     /locus_tag="VF633_09275"
     CDS             2979..>3835
                     /locus_tag="VF633_09275"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8471290.1"
                     /translation="MNTAAEIVAVLVIGTGLLQNLIYMIQLALAAASLIETPSTPSAN
                     VLWRRYADASPPIALLAPAYNEALTIVESVRSLLSLQYPNFEIVVINDGSSDETLKVL
                     TEAFGLEPIERNYDLAVPHAPITRLYGSSNQPRLVVVDKQNGGKADALNAGVNVSRAP
                     IICSMDADSLLEPDALLRAVRPFIDDPIRTVAVGGTVRIANGCRIDHGRVVEVRTPTN
                     PLALLQTVEYLRAFLMARLAWSRVNALTIISGAFGLFRRANVIEAGGYTRGTVGEDME
                     LVVKLHRLMR"
BASE COUNT          638 a         1225 c         1253 g          719 t
ORIGIN      
        1 cagtcgcccg accgaagtga ccggcccgga ggatcccctg gcggcgttga gccggcgtta
       61 cgccgaccac atcaagacgc tttcggcaga gatagatcgc ctgagcgaac agtcttttga
      121 acaacgggca gagggcttgg cgaacctggc gcatgccgta gcggggacag ccgggactct
      181 gggcttcccg tccctgtccg aggcggcatt tgaacttgag gactgcgcaa gacaggtgat
      241 ctccggcgac gtcgatggga cggcgctgga gcctgctgtc caggctttcc gcgatgcgtt
      301 gaatgtggag gcggcttcgc gactatgaca atggcctggc cgggacatct ttgctggtag
      361 aaggcgaaca aaacctcaaa aggccaagct tagccgtcaa accgtggcag atctgttaag
      421 atttctgtga ataggttcgc tcgtgcggga gcgctggagc gccctcggca gtaaactagt
      481 gcaggacagc aaacttgcat catccaaggc accgttcgga agctagagta tgtccgccca
      541 cgttcttatc gcagatgatg accctttctt gcgcgagttg ctggcccata aactgtctgc
      601 agcgggctac tccgtctcgt ctgcacggca cggtggcgaa gcccttgagc aatgccgctc
      661 ttcactgccc gacctgatcg ttctggacgc gatgatgccg gtcaccgacg ggtttgaggt
      721 cctgcgccga ctgaaatccg accctctcac ggctgcggtg ccggtgatta tgctaacggc
      781 catgaagcgc gaagaagacg tggtgggcgc actgaaactg ggcgcggccg actacctcgt
      841 gaaacctttc atccctgatg aactggtggc gcgcatcggg cgccttttgc ctacgggaac
      901 ggatcatgcc aacacccctg acgatcgcgg ttctggcttg tctcgcgcca attgagaccg
      961 cccgggccca ggatcaggca ggcggttccg agcgggttta tgaagcggcg gtagaggcgc
     1021 gcctcatggg tcgaacgaca gaggcgatcg agggatcacg ccgtgtcctg gccgtgcggc
     1081 ccaatgacgt cgatgcacgg ctgaacctgg cgctggccct tatcgccgct ggtcggctgg
     1141 acgaagcaga ggttgaactc gtcaccgtcc tcaaccaggc gcctgcgtat gaggacgcgc
     1201 gcgtagctct ggatagcatc catcgcctgc gtagggatcc ggttcgctgg cggctcgacg
     1261 tctcggccag ctatagcgat ctgtcgcaga atctcccgcc ttggcgcgag agcgccctga
     1321 ccatcagtcg caggacgatt catggctcgg tctcggggac gatcgaacac gcagaacgct
     1381 ttcgacgcca ggatacctat ttcgagggcc gccttgaccg ttccctcggt cgcggctcgg
     1441 tgtacggcgc cgtcggcgga accccgaacg ctgacttccg gccggagatc gcggtgcgag
     1501 gcggcgggca agcgcccgta tttggtcaga acctgtccgc caccctggac gtcggggtcg
     1561 cacgttacgc cgttggcgtc gtctcgacgg tccagcccgg tgtcgaatac accacgtcag
     1621 gcggggcgct gaccgtcggc gcgcgctgga tccacctgtg ggacgagcgg gacacctatc
     1681 gctccggcta cagccttcgc gctctgctgg ccatccgacc atccgttcgc gtcagagctg
     1741 gcttcgccga cgcccctgag tcttcggatg gattgaccgt cgatgtccgc gccgccagcc
     1801 tcggcgccga ggtcgatgtg aacgacaggc tcacgctgcg cctcaacggc ctgaaggaag
     1861 atcgcgcggc gtacgaccgc gacgaaatca cgctgggcgt cggcgttcgg ttctgaccgt
     1921 gaacccgctg atgctgatct ggtggacgtc gctcgtgctg gcaggcatcg ccttcagctg
     1981 gatgatgggg ttgatcgtcg cccggctgtt ccgcgagaac agcgaggccc ggcgcatcgc
     2041 ggatcaacag acgttgtccg ccgcgtatct gatgatcatg agcggggcag gtgaagcggg
     2101 aacgctgctt gagccctttc gcaaacgcag tcgtctgttg gccgaaagcc tgctcgaggt
     2161 tctgagtctt gtaagggggg tcgagcgtga acgtctgatc gccagcctca tcgacgccgg
     2221 cgtcgcggac cgtcttcggt cccggctgac gcgcggcagc aggaccggtc ggctggcggc
     2281 tgcagaagcg ctttcggcct tcccgtccat ggcgacccgc acggctttga gggcccttca
     2341 tcgccgctcg accgacacgg aaatccgcat cgccagcgtc cgcaccctga tcgacatcgg
     2401 agacccgccc aacgccacgg aattggtcgc cgatctggag ggcaagggtt acagcgaatc
     2461 tctcctgtac gcccccgtgc tgcgtcgcct tgccgttgac gcgcccgacg acgcccttga
     2521 gacgctgact cttcccggac ttgggatggc ggcgcgcgcc gtcatgatcg aggctgtcgg
     2581 agccagcggt gactatcggg ccttgcctgc gctatcgctg atggcggcag ggccggaacc
     2641 tggcttgcgg atgtccgccc ttcgcgcctt gggggccctg gcgcatcccg gcagcgccga
     2701 aaccatcgtc ggcgcgctcg gtgacgaggc gtgggacgtc cgcgccgagg cggctatggc
     2761 tgccggaagg attggtgggc cggcgcttgt ccaggcgctg acgccgctgc tggacgatcc
     2821 cgtctggtgg gttagattca gggcggccga gtccatgagc aagatgggcg gatccggcat
     2881 cgaggcgctg cgctacgcgg cgacgtcgca agtggatgtc acccggcggt ccgcttcgct
     2941 ggctctggcg gagcagggtc tggcatgacg gtcctgcctt gaacactgcc gccgagatcg
     3001 tcgccgtcct cgtgatcggc acaggtctgc ttcagaacct gatctatatg atccagctcg
     3061 cgctcgccgc cgcgtccctg atcgagaccc cctcaacccc ctcggccaat gtcctgtggc
     3121 ggcgctacgc agacgcttcc ccgccgatcg cgctactggc gccggcctat aacgaggctc
     3181 tgaccatcgt cgaaagcgtc cgctcgctcc tgtcgcttca gtacccgaat ttcgagatcg
     3241 tggtcatcaa cgacggttct tcggacgaga cgctgaaggt cctgacggaa gccttcggtc
     3301 tcgaaccgat cgaacgcaac tacgatctcg cggtgccgca cgcgccgatc acccgcctct
     3361 acggctcaag caaccagccg cgactggtgg tcgtcgacaa gcagaatggc ggcaaggcgg
     3421 atgcgctgaa cgccggggtg aacgtgtccc gtgcgcccat catctgttcg atggacgccg
     3481 actccctgtt ggagcccgat gcgctcctgc gagctgtgag gccgttcatc gatgatccga
     3541 tccgcaccgt ggcggtcggc ggaaccgttc gcatcgccaa cggctgtcgc atcgaccatg
     3601 gccgtgtcgt cgaggtccgg acgcctacca atcccctggc cctgcttcag acggtggaat
     3661 acctccgcgc cttccttatg gctcggctgg cctggagccg ggtcaatgcc ctgaccatca
     3721 tatcgggcgc gttcggcctc tttcgaaggg ccaatgtcat cgaggccggc ggctatacgc
     3781 gggggacggt gggcgaggac atggagctgg tggtcaagct gcaccgcctg atgcg
//
LOCUS       DASVTM010000341         4086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_670186,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000341 DASVTM010000000
VERSION     DASVTM010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670186"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VF633_09280"
     CDS             <1..1102
                     /locus_tag="VF633_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471291.1"
                     /translation="FFAEPFWGRLSDRIGRKPVLLITLAANAIGYLALAFVPNIWLAI
                     AVRLFTGLGAGNISTVQGYVADVTPPAERAGRMGMIGAAFGMGFIVGPGLGGLLTQPQ
                     LGHLGYQLPIFVASGLAALAALGVVVFLKESRAKADPAAPRPAFLTGLHDARANPVVS
                     RVLLVTLIYMAGFSAMESVFGLWAESRYAWGAREVGLSFMVVGIVSAINQGVLTGRLA
                     RRFGETRVLAIGMLLFGASLVLQVLAPAAWFPAFSLDLGALTVPIVQGWIIPIVMAVG
                     ACGMSLAMPNISALISRSTPPDRQGAMLGLNMATGSIARIFGPMIAGALFSGVGHDWP
                     FLVGAALTVPAAWMAINAGRALKLGASQTASV"
     gene            1262..1459
                     /gene="rpmI"
                     /locus_tag="VF633_09285"
     CDS             1262..1459
                     /gene="rpmI"
                     /locus_tag="VF633_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX8471292.1"
                     /translation="MPKLKTKSGAKKRFSFTATGKVKSGVAGKRHRLISHNSKYIRQN
                     RGTKVMADADAKKIKSYMPYA"
     gene            1570..1923
                     /gene="rplT"
                     /locus_tag="VF633_09290"
     CDS             1570..1923
                     /gene="rplT"
                     /locus_tag="VF633_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX8471293.1"
                     /translation="MARVKRGVVSHARHKKVLEQAKGFSGRRKNTIRTAKAAVDRAGQ
                     YAYRDRRVNKRNFRALWIQRINAAARQEGFTYSQFIHGLGKAGIELDRKVLAAIAMEG
                     VGFKEIADKVRAALA"
     gene            2065..3141
                     /gene="pheS"
                     /locus_tag="VF633_09295"
     CDS             2065..3141
                     /gene="pheS"
                     /locus_tag="VF633_09295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX8471294.1"
                     /translation="MTDLASLELELINAIGGAATVVEVEAVRVNALGKTGVISGLLKG
                     MGAMSPDERREQGPMINGLRDRVAAAISDKKTELEAAELDARLLSERLDLTLPAKPRR
                     KGGVHPTMQVMDEMVAIFASMGFAVAEGPDIEDDFHNFTALNFPPKHPAREMHDTFFL
                     KPDPETGERKVLRTHTSPVQVRTMMSQQPPIRIIAPGRTFRKDSDATHTPMFHQVEGL
                     VIDRDIHMGHLKTTLETFIARFFELDAVDARFRPHHFPFTEPSAEMDIRCDRSGGQIK
                     FNEGTDWLEILGCGMVHPNVLRACGIDPDEYQGFAFGMGVDRLGMLKYGMPDLRPMFE
                     ADVRWLAHYGFSAFSAPNVASGLS"
     gene            3152..3550
                     /locus_tag="VF633_09300"
     CDS             3152..3550
                     /locus_tag="VF633_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471295.1"
                     /translation="MTDIMSEAFGRELVKREIFTNEAVWLPLMAIHHLNAGDPVIRNK
                     TFTDCVIEGPSLVTPLGSTNFDSCNMGVAANPRSLLYKPMGPIMVGVIGLENCKFIRC
                     RMVQIGFTGADAFLDELSGQLVAAHGDHTA"
     gene            3547..3945
                     /locus_tag="VF633_09305"
     CDS             3547..3945
                     /locus_tag="VF633_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471296.1"
                     /translation="MTTRLPINRPMNIADDLSLVAFDNVDINLFDLYAAHQGQGAVIG
                     RTFTGCRLQGPAIVLVSGGTSFEDTNFGDPRGEMRNLLLQPMGSKALGTVPMVDCKFI
                     GCEFYNVGFTGSADILELLAAVPSLPGAPA"
     gene            3942..>4086
                     /locus_tag="VF633_09310"
     CDS             3942..>4086
                     /locus_tag="VF633_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471297.1"
                     /translation="MKFTLGWLKDHLETTADAAAVADAMTMAGLEVEHVTDPTLALAP
                     FTVA"
BASE COUNT          730 a         1343 c         1235 g          778 t
ORIGIN      
        1 gttctttgcc gagccgttct gggggcggct gtccgaccgg attggcagga agcccgtcct
       61 gttgatcacg ctggccgcca acgccatcgg ctatctagcg ctggcctttg tgcccaacat
      121 ctggctggcc atcgcggtgc gactgttcac gggcctgggc gcgggcaaca tctcgacggt
      181 tcaggggtat gtggccgacg ttacgccacc ggccgagcgc gccgggcgga tgggaatgat
      241 cggcgcggct ttcggcatgg gcttcatcgt cgggccggga ctgggtggct tgctgaccca
      301 acctcagctg ggtcatctcg gctatcagct gccaatcttc gtcgcctcag gactggcggc
      361 tctggctgcg cttggcgtcg tcgtcttttt gaaggaaagc cgcgccaagg cggatccggc
      421 agcgccgcgt cccgcctttc tcaccggcct tcacgacgcc cgcgctaatc ccgtggtgtc
      481 ccgggtgctg ctggtgaccc tcatctacat ggccgggttc tcggcgatgg agagcgtctt
      541 cgggctatgg gccgagagcc gatacgcgtg gggggcgcgg gaggtcggac tgagtttcat
      601 ggtcgtgggg atcgtctcgg cgatcaatca gggcgtgctg accgggcggc tggcgcgcag
      661 gttcggagag acgcgggtgc tggccatcgg catgctgctg ttcggcgcct ctctggtgct
      721 gcaggttctc gcgcccgccg cctggttccc ggcattcagc ctcgatctcg gcgctctgac
      781 cgtgcccatc gttcagggct ggatcatccc catcgtcatg gcggtcggcg cctgcggcat
      841 gtcgctggcc atgcccaata tctcggcctt gatcagtcga tccacgccgc cggaccggca
      901 gggcgccatg ctgggcctca acatggcgac aggatcgatc gcgcgtatct tcgggccgat
      961 gatcgccggc gcgctgtttt cgggcgtcgg ccacgactgg cccttccttg tcggtgcggc
     1021 cctgactgtt ccggctgcat ggatggcgat caacgccgga cgcgctctga aactcggcgc
     1081 ttcccaaact gcttctgtct agactccgct tgcctttgag cggtggttcg cctactagcc
     1141 gcgccttcgc ctacgaaccg ccgggctaat ggcatgcctt ggcggttctg aaccacacgc
     1201 taccgaccaa tcctccgggc tcatgcagtt cgacggatgg tagctcaaaa agagagtgaa
     1261 aatgccgaaa ctgaagacaa agtcgggcgc caagaagcgc ttttctttca ctgcgacggg
     1321 caaggttaag tccggggttg ccggcaagcg tcaccgcttg atcagccaca actccaaata
     1381 catccgtcag aaccgcggca ccaaggtcat ggctgacgcc gacgccaaga agatcaaatc
     1441 ctacatgccc tacgcctgag cgtagcgcac acaccgatct gacttcatcg cattgaattg
     1501 aaatagaagt ccctcgaacc gtcagccgcc gcgcgacgca cttcagggac gcttccgaag
     1561 gataacatca tggctcgcgt aaaaaggggt gtagtttctc acgcccgtca caagaaggtt
     1621 ctcgaacaag ccaagggctt ctcgggtcgc cgcaagaaca ccatccgcac ggccaaggcc
     1681 gccgtcgacc gcgctgggca atacgcctac cgcgaccgtc gcgttaacaa gcgcaacttc
     1741 cgcgccctgt ggatccagcg catcaacgcg gccgcccgtc aggaaggctt cacctactcg
     1801 cagttcatcc acggcctcgg taaagccggc atcgaactgg accgtaaggt cctggccgcg
     1861 atcgcgatgg aaggcgttgg cttcaaggaa atcgccgaca aggtccgcgc cgccctcgct
     1921 taaggcgaac gtgacgtaag actttcggtt cgaacgatca taaacgcccg ctccggacac
     1981 cggagcgggc gttttcgtgt ctggcgccat gatcaggccg cgcatgggtg acgaacggct
     2041 cgccagccgc tagatgctcc cacaatgact gatctcgctt ctctcgaact cgaactgatc
     2101 aacgccatcg gcggcgcggc taccgtcgtc gaggtcgagg ccgtgcgcgt caacgccctc
     2161 ggcaagacgg gcgtcatttc cggcctgctc aagggtatgg gcgcgatgag ccccgacgag
     2221 cgccgcgagc agggaccgat gatcaacggc ctgcgcgacc gcgtcgccgc cgccatcagc
     2281 gacaagaaga cagagctcga ggccgccgaa ctggacgcac gattgctgtc cgaacgcctc
     2341 gacctgaccc tgcccgccaa acctcgccgc aagggtgggg tgcatccgac catgcaggtg
     2401 atggacgaga tggtcgccat cttcgcgtcg atggggttcg ccgtcgccga aggcccggac
     2461 atcgaggacg acttccacaa ctttactgcg ctcaacttcc cgcccaaaca cccggcgcgg
     2521 gagatgcacg acaccttctt cctgaagccc gatcccgaga ctggcgagcg caaggtgctg
     2581 cggacccaca cctcgccggt gcaggtgcgc acgatgatgt cgcagcaacc gccgatccgc
     2641 atcatcgcgc ccggccggac gttccgtaag gattccgacg ccacccacac cccgatgttc
     2701 catcaggtcg agggtctggt gatcgatcga gacatccaca tgggtcacct gaagaccacg
     2761 ctggagacgt tcatcgcccg gttcttcgag ctggacgccg tggatgctcg cttccgccca
     2821 caccatttcc ccttcaccga gccctcggcc gagatggata tccgctgcga ccgctcaggc
     2881 ggccagatca agttcaacga ggggaccgat tggctcgaga ttctcggctg cggcatggtc
     2941 cacccgaacg tcctgcgcgc gtgcggcatc gaccccgacg aataccaggg cttcgccttc
     3001 ggcatgggcg tcgaccgctt gggaatgctg aaatacggca tgcccgacct gcgcccgatg
     3061 ttcgaggccg acgtccgctg gctcgcccat tacggcttct ccgccttctc cgcccccaac
     3121 gtcgcctccg gcctgagctg atcgagacac catgaccgat attatgtccg aggctttcgg
     3181 ccgcgagttg gtgaaacgtg agatcttcac caatgaagcc gtctggctgc cgcttatggc
     3241 cattcaccac ctgaacgcgg gcgatcccgt catacgcaat aaaacattca ccgattgcgt
     3301 gatcgaagga ccgtcgttgg tcaccccgtt ggggtcgacc aatttcgata gctgcaacat
     3361 gggcgtcgca gccaaccccc gatcgctgct ctacaagccc atggggccga taatggtggg
     3421 ggtcattggc ttggagaatt gcaagtttat ccgctgccgc atggtgcaga tcggctttac
     3481 gggcgcagac gccttccttg atgagttgtc cgggcagcta gtcgccgccc acggagacca
     3541 caccgcatga cgacgcgcct tccgatcaat cgaccaatga acatagccga cgacctttcg
     3601 ctggttgcgt tcgacaacgt cgatatcaac ctcttcgatc tttacgcggc ccatcaaggt
     3661 cagggagccg tgatcggccg gacctttact ggctgccgtc tgcaagggcc tgccatcgtc
     3721 ctggtgtcgg gtgggacatc attcgaggac accaatttcg gcgacccgcg cggcgagatg
     3781 cgcaacctgc ttctgcagcc tatgggctcc aaggctctgg ggaccgttcc catggtggat
     3841 tgcaagttca tcggctgcga attctacaac gtgggcttca ccggcagcgc cgacattctc
     3901 gaacttctgg ctgctgtgcc ttcgctcccg ggggcgccgg cgtgaaattc actctcggct
     3961 ggctgaagga ccatctggag acgacggccg atgcggccgc cgtcgccgac gccatgacca
     4021 tggcgggtct ggaggtcgaa cacgtcaccg atccgaccct ggccctggcc ccgttcaccg
     4081 tggctc
//
LOCUS       DASVTM010000342         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_350341,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000342 DASVTM010000000
VERSION     DASVTM010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350341"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..226
                     /locus_tag="VF633_09315"
     CDS             2..226
                     /locus_tag="VF633_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEX8471298.1"
                     /translation="MSGDAPANDPGFNLDVPGATRERPLTPAARRALEEAAERRMLEA
                     AEMAVEHGGPTGPEPTRYGDWEKRGLAVDF"
     gene            409..2241
                     /gene="typA"
                     /locus_tag="VF633_09320"
     CDS             409..2241
                     /gene="typA"
                     /locus_tag="VF633_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX8471299.1"
                     /translation="MNLRNVAIIAHVDHGKTTLVDQLLAQSGVFRANEAQTERAMDSN
                     DQEKERGITILAKCTSVLWHGAAGDTRINIIDTPGHADFGGEVERILGMVDGCVILVD
                     AEEGVMPQTKFVLTKALKMGLRPILCINKVDRPHADPDRVHNEAFDLFAAIGATDEQL
                     DFPHIYASGRNGWATMDLNVPNDTLAPLFDLIVSHVPPPKVQENKDKPFQILNVLIES
                     DPFLGRLLTGRIESGKAVPGMAIHALDRDGKEIERGRITKVLAFRGLKRQAVEEGAEA
                     GDIVAIAGMSKATVADTLCAMEVTTALPAQPIDPPTISMTVSVNDSPLAGREGDKVQS
                     RVIRDRLLKEAEANVAIRVTTTEGGDAYEVAGRGELQLGVLIENMRREGFEVSISRPR
                     VVYQTGENGEKMEPIEDVMIDVDDEYSGVVIEKLSARKAEMTDMGPSGAGKTRISLKA
                     PSRALIGYQGEFLTDTRGSGVLNRVFSHYEPHKGPIEGRLKGVLVSNSDGDTAAFALW
                     NLEDRGIMFVGANEKTYEGMIIGENARWDDLDVNPIKGKQLTNVRASGKDEAVRLTPP
                     RLMSLEQAIAYIGEDELVEVTPKSIRLRKTILNPSFRKKRARPE"
     gene            complement(2647..3033)
                     /locus_tag="VF633_09325"
     CDS             complement(2647..3033)
                     /locus_tag="VF633_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471300.1"
                     /translation="MKASVSLPGDRTQARREEHRKAVRLTRVLETMTATILRRLAERN
                     QPAGRFFWDGLVGEFGTSALVSTLSLRSADGSIVAEGRLRHEEWVFSIRVNLPDYALR
                     GVYAGLHDMLNLMSYSDLEMVPATVH"
     gene            3453..>4020
                     /locus_tag="VF633_09330"
     CDS             3453..>4020
                     /locus_tag="VF633_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEX8471301.1"
                     /translation="MARPINLLNARFVQTTKVVGLHADGGGLYLEVDKGGAKRWTFIW
                     RTGTTRRQMGLGGTLVTDLVDARAMADTARRQVAKGLDPIAERNAERASGRTFGEAAD
                     ELLISLKPQWRNPKHAYQWDYSLKTLAKPIRHLTVDAVTTQHMVELLKPIWGTINETA
                     SRTRGRIERVLDAERAAGNRSGENPARWK"
BASE COUNT          809 a         1347 c         1217 g          647 t
ORIGIN      
        1 catctcaggc gacgcacccg ccaacgatcc cggcttcaat ctcgacgtgc ccggcgcgac
       61 gcgtgaacga cccctgacgc ctgccgcccg tcgggcgctg gaagaagcgg cggagcggag
      121 aatgctggaa gccgccgaaa tggccgtcga gcatggcggc ccgaccggcc cggaacccac
      181 gcgctatggc gactgggaga agaggggcct cgcggtcgac ttctaaatcg ccgttacgtc
      241 gccgcaatcc tcgcttaaag agcccggagg ccggtagcgc acactccgaa acgctgccac
      301 tggccttttc gctcgcaccc ctgtatgagg cgcgcgctcc ccgaagtacg cacattcccg
      361 agcggccccc gcacttgccg gccgcctttt cgcattgaac gacccgacat gaaccttcgt
      421 aacgtcgcca tcatcgccca cgtcgaccat ggcaagacca ctctggtgga ccaactcctg
      481 gcccagtccg gcgtcttccg agccaacgag gctcagaccg aacgggcgat ggactccaac
      541 gatcaggaaa aagagcgcgg gatcaccatc ctggccaaat gcacctcggt gctgtggcat
      601 ggcgctgcgg gcgacacgcg catcaacatc atcgacaccc ccggccacgc cgacttcggc
      661 ggtgaggtcg agcgtattct cggcatggtg gatggctgcg tcatcctggt cgacgccgaa
      721 gagggcgtca tgccccagac caagttcgtg ctgacaaaag cgctgaagat gggcctgcgt
      781 ccgatcctct gcatcaacaa ggtcgaccgt ccgcacgccg atcccgaccg cgttcacaac
      841 gaagccttcg acctgttcgc cgccatcggc gcgacggacg agcaactcga cttcccgcac
      901 atctacgcct cgggccgcaa cggctgggcg acgatggatc tgaacgtgcc gaacgacacc
      961 ctggcgcccc tgttcgatct gatcgtcagc cacgtgccgc cgcccaaggt tcaagagaac
     1021 aaggacaagc cgttccagat cctgaacgtg ctgatcgagt ccgacccctt cctcggtcgt
     1081 ctgctgacgg gccgtatcga gagcggcaag gccgtccccg gcatggcgat ccacgcgctg
     1141 gaccgcgacg gcaaggaaat cgaacgcggc cggatcacca aggtcctcgc cttccgcggc
     1201 ctgaagcgtc aggccgtcga agagggcgct gaggccggtg acatcgtcgc catcgccggg
     1261 atgtccaagg ccaccgtggc cgacaccctg tgcgccatgg aagtcaccac cgccctgccg
     1321 gcccagccga tcgacccccc gaccatctcc atgaccgtct cggtcaacga cagcccgctg
     1381 gccggtcgcg aaggcgacaa ggtccagtcg cgcgtcattc gtgaccgcct gctcaaggaa
     1441 gccgaagcca acgtcgccat ccgcgtcacg accaccgaag gcggcgacgc ctatgaggtc
     1501 gccggtcgtg gcgaactgca actcggcgtg ctgatcgaga acatgcgccg cgaaggcttc
     1561 gaggtctcga tctcgcgtcc tcgcgtggtc tatcagaccg gcgagaacgg tgagaaaatg
     1621 gagccgatcg aagacgtcat gatcgacgtc gatgacgaat actccggcgt cgtcatcgag
     1681 aagctgtcgg cccgtaaggc cgaaatgacc gacatgggtc cgtcgggcgc cggcaagacc
     1741 cgcatcagcc tcaaggcgcc ttcgcgcgcc ctgatcggct atcagggcga gttcctgacc
     1801 gatacgcgtg gttcgggtgt cctgaaccgg gtgttcagcc actatgagcc gcacaagggc
     1861 cccatcgaag gccgcctgaa gggcgttctg gtctcgaact ccgacggcga cacggcggcc
     1921 ttcgccctgt ggaacctcga agatcgcggc atcatgttcg tcggcgccaa cgagaagacc
     1981 tacgaaggca tgatcatcgg cgagaacgcc cgttgggacg acctcgacgt caacccgatc
     2041 aagggcaagc aactgaccaa cgtccgcgcc tccggcaagg atgaagccgt ccgcctgacc
     2101 cccccgcgcc tcatgtcgct ggaacaggcc atcgcctaca tcggcgagga cgaactggtc
     2161 gaggtcacgc ccaagtccat ccgtctgcgc aagacgatcc tgaacccgtc gttccgcaag
     2221 aagcgcgccc gtcccgagta aggacaaggt ccttcgacag cgatcaggaa tggaacaggc
     2281 cgcccggtcc cggagcggcc tgtttgctgt ctgttctgat cctggacgga cgccctcgtg
     2341 gccctggtgt tgcgagcaac cgcggcgatg gctcaagcgt ttgcacgcga tggaacacga
     2401 aaagccgccc gtgatgagaa atccggcccg ggcgcggcgg gcgttcgcca ttatcctcgg
     2461 tttgatcgtg atcatagcga tcgcggctta tgttctggct ctggggcatc aagaaaccaa
     2521 ggcccccgcg ccggagcggg gcgacgggat cgctccgctt gaagcggcgg ttcccggatc
     2581 gtccaggccg caataacgtg cgctcagaaa tgggcggtca gatttgaccg gcaagacggt
     2641 ccggcgtcag tgaaccgtcg cagggaccat ctcaagatcg ctgtaggaca tcaaattcag
     2701 catgtcatga agtcccgcgt agacacctcg caaggcgtaa tcgggcagat tcaccctgat
     2761 cgaaaacacc cactcctcgt gccggagacg cccctcggcg acgatcgatc cgtcagcact
     2821 gcggagggac agggtgctga ccaaggcact tgtgccaaac tcccccacca gcccgtccca
     2881 aaagaatcgc ccggcaggct gatttcgctc tgcgaggcgc cgcaagatgg tggcggtcat
     2941 cgtttccagc acgcgggtca gcctcaccgc cttgcggtgc tcctctcgac gggcctgagt
     3001 gcggtcgccg gggagggaaa cagaagcctt catgccggga tttatgcccg gcaagagtta
     3061 acaccacgca actctcaatt cctgcatgcc acaggcttga cgatctctcg tgcccgaccg
     3121 atcgcatcca gtggcgtcgg cagatatctg cccgggccat ccgcgccagt ccgacgatga
     3181 aacgcgcgcc tgccaggtcc ggctgaaaat agcgcatccg ccaggacggg tcgttctgca
     3241 ccaaaggata acgcctggtg gagccgaggg cttcctgacc cctgtctata gtcatcaatg
     3301 ccagttaatc ggaccattga tcctattgat aaattttctt tgatggtccg tatggctcca
     3361 tacctctcca gcgcaatcca gagccggggt tagggtaacg gttagggtaa gccagacact
     3421 tgcaaacccc cgtgttcgtg caaggttcta ttatggcccg accgataaac ctcctcaacg
     3481 cccgcttcgt tcagaccacc aaggtggttg gtctccacgc cgacggaggg ggcctctacc
     3541 tcgaggtcga caaaggcgga gccaagcgct ggaccttcat ctggcgaacc gggacgaccc
     3601 gccgccagat gggcctcggc ggcacgctcg tcaccgatct cgtcgatgcg agggcgatgg
     3661 ccgacacagc ccggaggcaa gtcgccaagg ggctcgaccc gatcgccgag aggaacgcgg
     3721 agcgtgccag cggccggacc ttcggcgagg ccgccgacga gttgctcata tcgctcaagc
     3781 cccagtggcg gaacccgaag cacgcctatc agtgggacta cagcctcaag acgctggcga
     3841 agccgatcag gcatctgacg gtcgatgcag tgaccactca gcacatggtc gagctgctca
     3901 agccgatctg gggcacgatc aacgaaaccg cctcccggac gcgcggccgc atcgagcggg
     3961 tgctggacgc cgagagagcc gctggcaatc gttccggcga gaaccctgcc cgttggaagg
//
LOCUS       DASVTM010000343         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_725561,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000343 DASVTM010000000
VERSION     DASVTM010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725561"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VF633_09335"
     CDS             <1..453
                     /locus_tag="VF633_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX8471302.1"
                     /translation="RPGLFRPDVQRRLTAVLETSPENLVFTATGIAPGTSPPPGPSGF
                     ESNARGWDAGSAPPCVAIERLQLSNDDLAPWAASGVVLEIDPARWPRRDQGCVIDLVD
                     GSRLIRLYERADADRLYLRGGPGGLGTESALARDRTRRVAAVIARLDP"
     gene            505..909
                     /locus_tag="VF633_09340"
     CDS             505..909
                     /locus_tag="VF633_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8471303.1"
                     /translation="MKRERLALPRIPDSIDTFVGRRIANRRSALGLSQSALATRLGIS
                     FQQIQKYESASNRVSASRLHQIAIALALPVRQFFPDTPLDGASPDNGRAEALPFMTAS
                     PEGRIVANQFASIESQDARRAVATIVGALAAR"
     gene            932..1606
                     /gene="tsaB"
                     /locus_tag="VF633_09345"
     CDS             932..1606
                     /gene="tsaB"
                     /locus_tag="VF633_09345"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX8471304.1"
                     /translation="MKVLVIDTALNACTAAVFEDDRPLGVRSELMVKGHQERIAGLVR
                     DAVADAGGFDGIDRIGVTVGPGSFTGLRVGLSFAHGLGAALGAPVVGISTLAALARTQ
                     DNGIGPTAAIVDARRGQVYVQTFRDGVPAMEATALNLDAAREALSGDAWLWAGSGAAL
                     IDTQTHDAVTTSTPEALAALTIAADPARSPPSPLYLRAPDATPPTRLPGQPRPPAPKP
                     SVTHPA"
     gene            1636..2082
                     /gene="rimI"
                     /locus_tag="VF633_09350"
     CDS             1636..2082
                     /gene="rimI"
                     /locus_tag="VF633_09350"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX8471305.1"
                     /translation="MILSPAPEDGPVLAAIHAEAFHAPWAAAAFESLLSQAGVFAIRA
                     SNGFILCRVVVDEAEILTLAVRPSARRTGLGARLTEAAADFAATAGAERLFLEVAEDN
                     VAARALYDRTGFIETGRRRNYYAKADGSRGDALLLTRESSQPLPTR"
     gene            2106..>2198
                     /locus_tag="VF633_09355"
     CDS             2106..>2198
                     /locus_tag="VF633_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX8471306.1"
                     /translation="MDRIEKLCADRGMRMTEQRRVIARVLSNAED"
BASE COUNT          356 a          767 c          691 g          384 t
ORIGIN      
        1 cgtcccggtc tttttcgccc ggacgtgcaa cggcggctga ccgccgtcct cgagacctcg
       61 ccagagaatc ttgtcttcac cgcaaccggg atcgcgccgg gaacgtctcc gccccccgga
      121 ccgagtggct tcgaaagcaa cgcgcgcggc tgggacgcag gttcggcgcc gccctgtgta
      181 gcgatcgaac ggctgcagct ttccaacgat gatcttgcgc cctgggctgc atccggcgtg
      241 gtgctggaga tcgacccggc gcgctggcct cgccgggatc agggctgtgt catcgacctg
      301 gtcgacggct cacgcctgat ccgtctctat gagcgcgccg acgccgaccg attgtaccta
      361 cgcgggggtc cgggggggct gggtaccgaa agcgcgctcg ctcgcgatcg cacccgccgc
      421 gtcgccgccg tcatcgcgag actggatcct tgatgaaacg aataagttgc tatgagtttc
      481 gtttcatgaa acgatggctt tcgaatgaaa cgggagcgcc tggccttgcc gagaattccg
      541 gacagcatcg acaccttcgt tggccgccgc atcgccaatc gtcggtctgc ccttggcttg
      601 tcgcagtcag cgctggctac gcgactcggc atcagctttc agcagatcca gaaatacgag
      661 tcggcatcga accgggtctc ggcctcccgc ctccatcaga tcgcgatagc gctggccttg
      721 ccggtgaggc aattctttcc ggacacgcct ctggatggcg cttcgccaga caacggccgg
      781 gccgaggctc tgcctttcat gacagccagc ccggaaggtc gcatcgtagc caatcagttc
      841 gcttcaatcg aaagtcagga cgcccggcga gcggtagcga ccatcgtcgg ggcgttggcc
      901 gcacgctgaa ccacactgcg gatgtccctg catgaaggtc ctcgtcatcg acaccgccct
      961 gaacgcgtgc accgccgctg tgttcgaaga tgaccgaccg ctcggcgttc gcagcgaact
     1021 catggtcaag ggccatcagg agcggatcgc cggccttgtc cgtgacgccg tcgcagacgc
     1081 gggcggcttc gacggtatcg accggatcgg cgtcactgtc ggacccggct cattcactgg
     1141 cctgagggtc gggctgtcgt tcgcccatgg attaggcgcg gcgcttggag cgccggtcgt
     1201 cgggatttcg acgctcgccg ccttggcccg gacgcaagac aatggaatcg ggccgaccgc
     1261 cgccattgtc gacgctcgca gggggcaggt ctatgtccag acgttccgcg acggcgtacc
     1321 ggcgatggag gcgacggccc tcaacctcga tgctgctcgt gaggctttgt ccggcgacgc
     1381 ctggctctgg gcaggaagcg gcgccgcatt gatcgacacc cagacgcacg acgcggtcac
     1441 gacctctacg ccggaagcgc tcgccgccct gaccatcgcc gctgatcctg cgcgctcgcc
     1501 gccgtcgccc ttgtatctac gcgcgcccga cgccaccccg ccgacgcgcc tgcccggcca
     1561 gcctcgcccg cctgcgccca agccctcggt cacgcacccc gcgtgacgga cccggcgctc
     1621 agcatcgaac ccgacctgat cttgtctccg gcgccggagg acggtcccgt gctggctgcg
     1681 atccacgccg aagccttcca cgccccgtgg gctgccgccg cattcgaaag cctgctgtcg
     1741 caggcaggcg tgttcgccat ccgcgcgtca aacggcttca tcctttgccg ggtcgtcgtc
     1801 gatgaggcag aaatcctgac cttggctgtg cgtccatcgg cccgccgaac cggtctcggc
     1861 gcgcggctca cggaggccgc tgcggatttc gccgctacgg ccggagccga acgcctgttc
     1921 ctcgaagtcg ccgaggacaa cgtcgctgcc cgcgccctct atgaccgaac gggcttcatc
     1981 gagaccggac ggaggcgaaa ctactacgca aaagccgatg gaagccgtgg cgatgcgctt
     2041 cttctgacac gcgagtcgtc acagccgctt cccacacgat gacgctgcct ctatcgtgtc
     2101 gaaccatgga ccggatcgaa aaactttgcg ccgaccgcgg catgcggatg accgagcagc
     2161 ggcgcgtcat cgcgcgagtg ctgtcgaatg ccgaggac
//
LOCUS       DASVTM010000344         4676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_779402,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000344 DASVTM010000000
VERSION     DASVTM010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779402"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VF633_09360"
     CDS             <1..603
                     /locus_tag="VF633_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX8471307.1"
                     /translation="TWFRRDRAAFDTFARELLPAIGRVRENGLVRVWSAGCSNGQEAW
                     SLAMGALDAGANVDILATDLSQRAVDRARTAAYTTFEIQRGLSAATMLRWFEPADEQW
                     SARAELRKVVKFERANLLDEPAEVPSRFDVIFCRNVLADIEPSRRAMVIESLERRLVD
                     DGCLFLGADERLDGDSVAFRPVNGRRGLFVKAPSALSRAA"
     gene            681..2561
                     /locus_tag="VF633_09365"
     CDS             681..2561
                     /locus_tag="VF633_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8471308.1"
                     /translation="MRLALLLGSLALALLIGVIALQTPSPRGLQTPAVAFSAGRAMGD
                     VNVIARKPHPLGSSEHEAVRGYLFGRMTTLGLSPTLQSGPLSPAAVKRLQRWNLDPNK
                     PAINLIGVLPGRDPAAPAVLLMAHYDTVAVSPGAADDSAGVAAILEAVRAIKARGPAE
                     RSLVVLFTDGEELSLDGARNFFSDHPLRDHIGAVVNLEARGGGGRAMMFETGPRNRQT
                     IDLFTNAVAKADGGATSNSLAVLIYELMPNGTDFTIPRQRSLPGINLAFIGRPAQYHS
                     ATSTPAALDQGSVQHIGSQALEMTDALLRAPALPVAAEKRVYADVLDHLIIAHAPATG
                     WILLAVTAALGLFAFWGGRHATGLTAADVGRGLLGGLWFLGTSIVLVQAVRLLAGPLS
                     SRAESADAYYVLLRRLPWMEGGVAMTILAVALAALAGRDIVGRRVLAGVVIAATLLAT
                     VLDGFNPVTIGAAIVAVGLSLWSQATPRSVWGGWLGLIGLISILGAGVQAFAPEAAFL
                     LIWPALIAAAAAGFAAVIGARLVSKFSLIPAAVIGAVGGGWIVSLAHSVFLGVGMDIP
                     AVLAPLGLLILMFVRPLAPPQVARALAGCAAAALIFACGLSMAARYAEPMQAAVAVTP
                     TT"
     gene            complement(2565..2714)
                     /locus_tag="VF633_09370"
     CDS             complement(2565..2714)
                     /locus_tag="VF633_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471309.1"
                     /translation="MTCDHEPDEHDYRALTATGNLTAFLHRHNRALIIIGAVAVVAAV
                     AAVIL"
     gene            2930..4012
                     /gene="recA"
                     /locus_tag="VF633_09375"
     CDS             2930..4012
                     /gene="recA"
                     /locus_tag="VF633_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX8471310.1"
                     /translation="MASQAALKLVGKDDGDKQRALEAAIAQIDRAFGKGSVMKLGKGG
                     VVAAIPSVSTGSLGLDIALGIGGLPRGRVIEVFGPESSGKTTLALHTVAEIQKAGGTA
                     AFVDAEHALDPGYAQKLGVNLDDLLVSQPDTGEQALEIVDTLVRSGAVDIVVIDSVAA
                     LTPRAEIEGEMGDSLPGLQARLMSQALRKLTASISKSNCIVLFINQIRHKIGVMYGSP
                     ETTTGGNALKFYASVRLDIRRTGAIKDRDEVVGNTTRVKVVKNKVAPPFREVIFDIMY
                     GEGISKLGEIIDLGVKAGIIEKSGSWFSYDSTRIGQGRENARAFLKANPDMADAIEDR
                     VRKSTNKIADELLGTPEPDEGQDLEG"
     gene            4329..4529
                     /locus_tag="VF633_09380"
     CDS             4329..4529
                     /locus_tag="VF633_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471311.1"
                     /translation="MADAIQKLTQFNLAETDDGYLLEIGGDDGGFLTVEATAEQLDAI
                     IEALDALISPEDDAVDGDEAED"
BASE COUNT          754 a         1591 c         1500 g          831 t
ORIGIN      
        1 acctggtttc gccgcgaccg ggctgcgttc gacaccttcg ctcgcgaact cctgcctgcc
       61 atcggcaggg tccgtgaaaa cggtctcgtg cgcgtctggt cggctggttg ctccaacggc
      121 caggaggcct ggtccctcgc gatgggcgcg ctcgatgccg gcgccaatgt cgatatcctg
      181 gctacggatc tgtcccaacg cgccgtcgat cgcgcccgaa ccgccgccta caccactttc
      241 gagatccagc gcggcctgtc ggcggcgacc atgcttcgct ggttcgagcc cgccgacgag
      301 caatggagcg ccagggcgga gctgcgaaag gtcgtcaagt tcgaacgcgc caacctgctc
      361 gatgagcccg ccgaggtccc ttcccgtttt gacgtcatct tctgccgcaa cgtcctcgcc
      421 gacatcgagc cgtcgcgaag ggccatggtc atcgagtccc tcgagcgccg actggtcgac
      481 gacggctgcc tgttcctcgg cgcagacgag cgtctggacg gcgatagtgt cgcgttccgt
      541 ccagtgaacg gtcgccgcgg cctgttcgtg aaagcgccct cggcccttag ccgggccgcc
      601 tgagccgcct ctactaggcc aagcttccca agaggacgtt cgcgccccta cagtctccct
      661 tctgtatgga ggggatcaac atgcggctcg ccttgcttct cggctcgctg gcgctcgcgc
      721 tgctcatcgg cgtcatcgct ctgcagacgc catcacccag aggcctgcag accccggccg
      781 tcgccttctc cgccggcaga gcgatgggcg acgtcaacgt catcgcccgg aaacctcacc
      841 ctctgggatc gtccgagcac gaggcggtgc ggggttatct gttcggacgg atgaccaccc
      901 tcggcctctc gcccaccctg cagagcgggc cattgtcgcc tgccgccgtg aagcggctgc
      961 agcgctggaa cctcgacccg aacaagccgg cgatcaacct gatcggcgtc ctgcccggac
     1021 gtgatccggc agcgcccgcc gtcctgctga tggcccacta cgacaccgtc gccgtttcgc
     1081 cgggtgcggc tgacgactcc gcaggcgtcg cggccattct ggaagccgtt cgcgccatca
     1141 aggctcgggg gcccgccgag cggtcgctgg tcgtgctgtt caccgacggc gaggagttga
     1201 gcctggacgg cgcgcgcaat ttcttcagcg accatccact gcgcgatcat atcggcgccg
     1261 tcgtcaatct ggaggcgcgc ggcggtggcg ggcgcgcgat gatgttcgag accggtcccc
     1321 gcaatcgcca gaccatcgac ctgttcacca atgccgtcgc caaggccgac ggcggtgcga
     1381 ccagcaactc actagcggtg ctgatctatg aactgatgcc caacggcacc gacttcacca
     1441 ttccgcgcca gcgcagcctg ccggggatca acctcgcctt catcggccgc ccggcccagt
     1501 accattccgc gacatcgacg cccgccgccc tcgatcaggg cagcgtccag cacattggtt
     1561 cacaggcgct ggagatgacg gatgccctgt tgcgggcccc tgccctgcct gtcgccgcag
     1621 agaagcgcgt ctacgccgac gtgctcgatc atctgatcat tgctcatgct cctgcgaccg
     1681 gctggatctt gctggcggtc acggcggctc tgggcctgtt cgccttctgg ggcggtcgtc
     1741 atgcgacggg cctgacggca gccgacgtgg gccggggcct gctcggcggg ctgtggtttc
     1801 tggggacgag catcgtcctg gtgcaggccg tccgtctgct ggccgggcca ttgtccagcc
     1861 gcgccgagtc cgccgacgcc tactacgtgc tgctgcgtcg gttgccgtgg atggagggcg
     1921 gggtcgcaat gacaatcctc gcggtcgcac tcgcggcctt ggccgggcgt gacatcgtcg
     1981 gacgtcgggt gctcgcgggg gtcgtcatcg ccgccaccct gttggccacg gttctggacg
     2041 ggttcaaccc ggtcacgatc ggagcagcca ttgtcgctgt cggcctcagc ctgtggtcgc
     2101 aggccacgcc gcgttcggtc tggggcggat ggttggggtt gatcggtttg atcagcattc
     2161 tcggtgcggg ggttcaggcc tttgcgcccg aagcggcctt cctgctgatc tggcccgcat
     2221 tgatcgcagc ggccgcggcg gggttcgcgg cagttatcgg cgcgcgactg gtcagcaaat
     2281 tctccctgat ccccgctgct gtgatcggcg ccgtcggcgg aggctggatc gtcagtctcg
     2341 cccactccgt cttcctcggc gtaggcatgg atatcccagc cgttctggcg ccgctcggcc
     2401 tgctgatcct gatgttcgtc cgccccctcg ccccgcctca ggtggccaga gcgctcgcag
     2461 gctgtgctgc ggcggctctc atcttcgcct gcggcttgtc gatggccgcg cgctacgcag
     2521 agcccatgca ggcggctgta gcggtaacgc ccacgacctg atcgctagag gatcactgcc
     2581 gccactgctg cgacgaccgc aacggctccg atgatgatca gcgcacggtt gtggcgatgc
     2641 aggaaagcgg tcaaatttcc ggttgcggtc agcgcgcgat agtcgtgttc atcaggttca
     2701 tggtcgcagg tcatgtgaac caaacgagcc cgagccacgg ctgttgcgac acacgacgtt
     2761 gcagggcgca cgtcaatatc ggtcgcaccg gccccggtgt tccaccattg ttctcaggaa
     2821 cgtattggca accagccgaa agcgtgttct acctctgttc atagggagcg ggtccatggc
     2881 gggtctctcc caagcatcga tgggaatcat ggcaagggag acgggtaaag tggcttcaca
     2941 ggcagcattg aaattggtcg gtaaagacga cggcgacaag cagcgcgctc tggaggccgc
     3001 gatcgcacag atcgatcgcg ccttcggcaa gggctcggtg atgaagctcg gcaagggcgg
     3061 ggtggtcgct gcgatcccct cggtctcgac cgggtcgctc ggactggata tcgcactcgg
     3121 catcggcggc ttgccgcgcg gtcgggtgat cgaggtcttc ggtcctgaat catcgggcaa
     3181 gacgaccctc gcgctgcaca ccgtggccga gatccagaag gctggcggca ccgccgcctt
     3241 cgtcgacgcc gaacacgccc tcgacccggg ctatgcgcaa aagctgggcg tcaatctcga
     3301 cgacctgctg gtgtcgcagc ccgacacggg tgaacaggcg ctggaaatcg tcgacacctt
     3361 ggtccgttcg ggcgccgtgg acatcgtggt gatcgactcg gtcgccgccc tgacgccacg
     3421 ggctgaaatc gaaggcgaga tgggcgacag cctgccaggt cttcaggctc gtctgatgag
     3481 ccaggcgctg cgcaagctga ccgcctcgat ctccaagtcg aactgcatcg tgctgttcat
     3541 caaccagatc cggcacaaga tcggcgtcat gtacggttcg ccggaaacga cgaccggcgg
     3601 taacgcgctg aagttctacg cctcggtccg tctcgacatc cgccgcaccg gcgcaatcaa
     3661 ggaccgcgat gaggtcgtcg gcaacaccac ccgggtcaag gtggtcaaga acaaggtcgc
     3721 ccctccgttc cgtgaagtca tcttcgacat catgtacggc gaaggcatct ccaagctcgg
     3781 cgagatcatc gacctcggcg tcaaggccgg gatcatcgag aagtcgggca gctggttctc
     3841 atacgactcg acccgcatcg ggcagggtcg ggagaacgcc cgcgccttcc tgaaggcgaa
     3901 ccccgacatg gccgacgcca tcgaggaccg ggtccggaaa tccaccaaca agatcgccga
     3961 cgaactgctg ggcacgcccg agccggacga aggtcaggat ctggaagggt agcaatcctc
     4021 ccccgttggg ggagggggac cgcgtcttcg cggtggaggg ggcctgatcc ggacacggat
     4081 gttggcttgc cccctccgtc gtgatgcaaa gctgcgcccc gccacctccc ccaatgggga
     4141 ggaacagaca ctcgttctgg cctcggtccc cgtgacccgc caccgacccc gcggggttcg
     4201 gaccagagcg agcagggccg ccccgcgcaa gcgaggcggc cctttttctt gcctgccaaa
     4261 ctcacttctt gctcccatct ggcgtgtggg atgctaactc gctggacgaa cctcccggag
     4321 ccctcctcat ggccgacgcc atccagaaac tgacccagtt caatctcgcc gagaccgacg
     4381 acggctatct gctcgaaatc ggcggcgacg atggcggctt cctgaccgtc gaagcgactg
     4441 cggaacagct cgacgccatc atcgaagccc tcgacgccct gatctccccc gaagacgacg
     4501 ccgtcgacgg cgacgaggct gaagactagg cgacgaccgg acgcgccacc ggccggcgca
     4561 gggcctgatc tatcgaggcg gggtcgcgta ccaccgctcg accgccgcag cccgccacgg
     4621 cacgggggtg atgcgtcatc gccaaaaccc ctgtatgagg cgcttccgcc tgttcg
//
LOCUS       DASVTM010000345         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_33904, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000345 DASVTM010000000
VERSION     DASVTM010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33904"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF633_09385"
     CDS             <1..425
                     /locus_tag="VF633_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8471312.1"
                     /translation="LTDRVLPAMRSAGWGRILNVASVSLIEPIGTLGVSNTLRASIAA
                     WAKTLSNEVAADGVTVNTLLPGRIDTPRIERLDKARAEQTGQTPEQARADAVTSIPVG
                     RVGTTEEFGAVAAFLASPLAAYVTGSLVRLDGESVRAV"
     gene            complement(438..1337)
                     /locus_tag="VF633_09390"
     CDS             complement(438..1337)
                     /locus_tag="VF633_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX8471313.1"
                     /translation="MLFAWLHHLAHERRLSPRTLQAYGHIGRHYLGFLERHRGEPQSL
                     ADLGTVTAAEVRAHLADRRSGDNPLNARSLGQTLAAIRGFHDFLDRRCGTPAPQLALV
                     RGPKIKPSLPRPVTADQARGMLQEPEADPDAEPWEAARDRAVLTLLYGCGLRISEALS
                     LIRADAPLPETLRIVGKGSKTRITPILPAVRAAVDTYLALQPFVLEPRDALFRAKRNG
                     PLSPRHVQATVQKLRGRLGLPASATPHALRHSFATHLLGAGADLRSIQELLGHASLST
                     TQKYTAVDAAHLLGAYAAAHPRA"
     gene            complement(1379..>1554)
                     /locus_tag="VF633_09395"
     CDS             complement(1379..>1554)
                     /locus_tag="VF633_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX8471314.1"
                     /translation="DRAADPEADPSVVGARHAICAFGSPEEDGFTAQMGCELVAFIAR
                     VVERTAERWPILN"
BASE COUNT          254 a          509 c          553 g          238 t
ORIGIN      
        1 gtctgaccga ccgggtattg cccgcgatgc gatctgcagg ttggggccgc atcctcaacg
       61 tcgcctcggt gtcgctgatc gagccgatcg ggacgctggg cgtgtccaac accctgcgcg
      121 cctccatcgc cgcctgggcc aagaccctgt cgaacgaggt cgccgccgac ggcgtcaccg
      181 tcaacaccct tctgcccggc cgcatcgaca ccccgcgcat cgagcggctg gacaaggcgc
      241 gcgccgaaca aacgggccag acgccggaac aggcgcgagc cgatgccgta acctcgattc
      301 cggtcggtcg cgtcggcacg actgaggagt tcggcgccgt cgccgccttc ctcgccagtc
      361 cattggccgc gtacgtcaca gggtcgctgg tgcgtctcga tggcgagtca gtgcgagcgg
      421 tttaactgta acggccgcta ggcccgggga tgggcggcgg cataggcacc cagcagatgc
      481 gcggcgtcga cggcggtgta tttctgcgtc gtcgacagac tggcgtgacc cagcagttcc
      541 tggattgaac gcaggtccgc tccggctccc agcagatggg tggcgaagga atgccgcagg
      601 gcatgcggcg tggcgctggc gggcaggccg aggcgaccgc gaagcttctg caccgtcgcc
      661 tgcacatgac ggggactcag gggaccgttc cgtttggccc ggaacagggc gtcgcgcggc
      721 tccagcacaa agggctgcag cgccagataa gtgtccaccg cggcgcgcac ggcaggcagg
      781 atcggcgtga tccgggtctt ggatcccttc ccgacgatcc ggagggtctc aggaagcggc
      841 gcatcggcgc ggatcaggct caacgcttcg gagatacgca ggccgcaacc atagagaagg
      901 gtcaggacgg ccctgtcacg ggcggcctcc cacggctcgg cgtcgggatc ggcttcaggc
      961 tcctgcagca tcccgcgagc ttgatcggcg gtgaccgggc ggggcaggga gggcttgatc
     1021 ttggggcctc ggaccagggc cagctgcggg gcgggcgttc cgcaccgtcg gtcgaggaag
     1081 tcgtgaaatc cccgtatggc ggccagcgtc tgacccagag accgggcgtt tagcggattg
     1141 tctccactgc gccggtcggc cagatgcgcg cgcacctcgg cggccgtcac ggttcccaga
     1201 tcggccagcg actgtggctc gccacgatgg cgttcgagga agccgagata gtgccgccca
     1261 atgtggccat aggcctgaag cgtccggggc gacagccgcc gctcgtgcgc cagatggtgc
     1321 agccaggcga acagcatctc accagatggt gtaatcccgc cggtcgctga ggggagggtc
     1381 agttcaggat cggccatcgt tcagccgtgc gttcgacgac ccgggcgatg aaggcgacca
     1441 gttcgcagcc catctgggcg gtgaacccat cttcctcggg tgatccgaag gcgcagatcg
     1501 cgtgacgcgc accgacgacc gacggatcgg cctcagggtc agccgcgcga tcgc
//
LOCUS       DASVTM010000346         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_513767,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000346 DASVTM010000000
VERSION     DASVTM010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513767"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..1669
                     /locus_tag="VF633_09400"
     CDS             425..1669
                     /locus_tag="VF633_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8471315.1"
                     /translation="MKVIIFGGDGFCGWPTALHLSARGWDVVIVDNLSRRNIDNELEV
                     QSLTPIRTMGERISAWKELTGQTIGFVNMTIGKEFDRLVALIRDEKPDSVVHFAEQRA
                     APYSMKSARHKLYTVDNNINATHHLLAAIVESGRDVHLAHLGTMGVYGYGTAGLKIPE
                     GYLKVTVETDNGPTEQEILFPPNPGSIYHMTKTQDALLFQFYARNDRLRITDLHQGIV
                     WGAQTEQTRMDERLINRFDYDGDYGTVLNRFLMQAAVGYPLTVHGAGGQTRAFIHIQD
                     TVRCVELALKNPPARGERVKILNQMTESRRVRDLAALVAQMTGATVEMVANPRQEADE
                     NELVVANDQFRDLGLKPITLAEGLMADVTDVARRYADRADLAKIPCVSAWNADRAEAL
                     EAATCPGPSAPGVTPIRPRVRA"
     gene            1669..>2380
                     /locus_tag="VF633_09405"
     CDS             1669..>2380
                     /locus_tag="VF633_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8471316.1"
                     /translation="MAEPVALLVFGGGYLGRAAALEAILGGGTAIATSRDPARRAALT
                     AAGIAAIDPDDRDALSTALAAATAVLVTAPPDANGCPALRALAAAESLATAWPDWIGY
                     ISSTAVYGDRNGGWVFEGDALNAASVEGARRVRAEADWFDAGRGMGLTVQVFRLPGIY
                     GPGRSVIDRLRDGSARLVRKPGQVFNRVHVDDAVAGLFASMANPRPGAAYNLVDDEPA
                     PADVVMAWAAERLGLPRPP"
BASE COUNT          406 a          804 c          766 g          404 t
ORIGIN      
        1 agatcgaaaa gatatgcggg acctcgccgg atggagacac tcgacccgta tcctctcgac
       61 ccttcccgac ccccaaccgt ggaaacccgc cccggcgtcc gatgcgcgtc gcggctttcg
      121 gcccatcacc cctgatggcg ggagtataat gcgattggag gcctccgttg ggcctctcgc
      181 ctgaccggtc tttgacatcg cggcttcgcg tgagccgcct catcatgcgc cagaccgtcg
      241 caggtctcgc gttaccgaag ctgctatgcg acggtccagt gtctccacaa tcccgctttc
      301 cggcgttttt ccgaaaccgg agacactcga ccctgcgccc tgtcgccaac gcccggtata
      361 gccccgcttt gccccgcccg ttctcatgcg ttaggcagag ggcagggcag gaaaaggcag
      421 ctagatgaag gtcatcattt tcggcggcga cggtttctgc ggctggccga cggccctgca
      481 tctgtcagcg cgcggctggg atgtggtgat cgtcgacaac ctcagtcgcc gcaacatcga
      541 caacgaactc gaggtccagt cgctgacgcc gatccgcacc atgggcgagc gcatctcggc
      601 ctggaaggaa ctgaccggcc agactatcgg cttcgtcaac atgacgatcg gtaaggagtt
      661 cgaccgactg gtcgccctga tccgtgatga aaagcccgac agcgtcgtcc atttcgccga
      721 acagcgcgcc gcgccctatt cgatgaagtc ggcgcgccac aagctctaca ccgtcgacaa
      781 caatatcaac gccacccacc acctgttggc ggccatcgtc gagagcggtc gcgacgtgca
      841 tctggcgcac ctcggcacga tgggcgtcta tggctacggc accgccggac tgaagatccc
      901 cgagggctat ctgaaggtca cggtcgagac cgacaacgga ccgaccgagc aggaaatcct
      961 gttcccgccg aacccgggtt cgatctatca catgacgaaa acgcaggacg ccctgctgtt
     1021 tcagttttac gcccgcaacg atcgcctgcg gatcaccgac ctgcatcaag gcatcgtttg
     1081 gggcgcccag accgaacaga cgcggatgga cgagcggctg atcaaccggt tcgactatga
     1141 cggcgactac ggcacggtgc tgaaccggtt cctgatgcag gcggcggtcg gctatcctct
     1201 gacggtccac ggcgccggcg gccagacccg ggccttcatc cacattcagg acacggtccg
     1261 ctgcgtcgag ctggcgctga agaaccctcc ggcacgtggc gagcgggtca agatcctcaa
     1321 ccagatgacc gagagccgcc gggtgcgtga tctggccgca ctggtcgccc agatgaccgg
     1381 cgccacggtc gagatggtcg ccaatccgcg tcaggaagcg gacgagaacg agctggtcgt
     1441 ggccaatgac cagttccgcg atctcggcct gaagcccatc accctcgcag agggactgat
     1501 ggcggacgtc accgacgtgg cgcgtcgtta tgccgaccgc gccgatctgg ccaagatccc
     1561 gtgcgtctcc gcctggaacg ccgaccgggc cgaggcgctg gaggcggcga cctgtcccgg
     1621 accatcagcg cccggggtga cgccaatacg accgcgagtt cgcgcctgat ggcggagccg
     1681 gtcgctcttc tggtcttcgg cggtggctat ctgggccgag ccgctgcgct ggaggctatc
     1741 cttggtggcg gcacggcgat tgcgacctcg agggatccgg cgcgtcgtgc agcgctgacg
     1801 gcggctggca tagcggctat cgatccagac gatcgcgatg ctctctccac cgccctagcg
     1861 gcagcgaccg cggtgctggt gaccgcaccc ccggacgcca acggctgccc ggccctgcgc
     1921 gctctcgcag cggcggaatc gctggcgacg gcatggccgg actggatcgg ctacatctcc
     1981 tcgaccgctg tctatggcga ccggaacggg ggctgggtct tcgagggcga tgcgctcaac
     2041 gccgccagcg tcgaaggcgc gcgccgggtt cgggccgagg ccgactggtt cgacgccggg
     2101 cgcggcatgg ggctgacggt gcaggtcttc cgtctgcccg gcatctacgg ccccggtcgg
     2161 tcggtgatcg atcgcttacg cgacggctcg gcgcgattgg tcaggaagcc cggacaagtc
     2221 ttcaaccggg tgcatgtcga cgatgcggtc gccggcctgt tcgcctcaat ggccaacccc
     2281 cgtccgggcg ccgcctacaa tctcgtcgac gatgagcctg cgccagcgga tgtggtgatg
     2341 gcttgggctg ctgagcggct aggtttgccc cgccctcccg
//
LOCUS       DASVTM010000347         7915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_725880,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000347 DASVTM010000000
VERSION     DASVTM010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725880"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /gene="argB"
                     /locus_tag="VF633_09410"
     CDS             <1..423
                     /gene="argB"
                     /locus_tag="VF633_09410"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX8471317.1"
                     /translation="EPTRVDPKLIAGLIASETDDWVPVIAPIGVADDGQTYNVNADTV
                     AGAIAGSLNAKRMLLLTDVKGVLDKNGDIIRQMSVAEAQGLIDDGTASGGMIPKLETA
                     MAAVRAGVEAVVILDGRRPHAMLVELFTEYGAGTLVKG"
     gene            420..1121
                     /locus_tag="VF633_09415"
     CDS             420..1121
                     /locus_tag="VF633_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX8471318.1"
                     /translation="MSADPVESTELSADLTHVRSWIFDLDNTLYPPESQFMRLVEKRI
                     NDYVVRTSGLPGPEALIVQKGYLREYGTSLAGLMAHYRIDPHDFLAEVHDVPLDVLTP
                     DAGLRAGLERLKGPRLVFTNGSAGHAKRVLDRLALSDLFDGVFALEDADLIPKPDPRT
                     FARMTERFGVSAASAAFFEDTARNLKPAKAIGMTTVLVGVEAVASPDAASADYIDFRA
                     AALGPFLATAHLHET"
     gene            1129..1998
                     /gene="dapD"
                     /locus_tag="VF633_09420"
     CDS             1129..1998
                     /gene="dapD"
                     /locus_tag="VF633_09420"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX8471319.1"
                     /translation="MTLDRSQLATVIEQAWDDRAELSINTHGEVRDAVEQSLALLDSG
                     EARVASRGDDGVWTTHQWLKKAVLLSFRINENKIMRAGEHGVPSSTSGAPGVGPFWDK
                     VPNKFGDWTAADYQKAGFRSVPGAIVRQGAHIARNVVLMPSFVNIGAFVDEGSMVDAW
                     ATVGSCAQIGKNVHLSGGAGIGGVLEPLQANPTIIEDGCFIGARAEVAEGVIVREGAV
                     LAMGVYLSGSTRIVDRATGEVFRGEVPAYSVVVPGAMPDPKGGPSLYCAVIVKRVDAQ
                     TRAKTGVNELLRD"
     gene            2222..5683
                     /locus_tag="VF633_09425"
     CDS             2222..5683
                     /locus_tag="VF633_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471320.1"
                     /translation="MTTLRVRALQCASTTALVVGLLSAQPVAAQTAPSEPQAGSSDEA
                     AQVDEVVVTGIRGALRSALNTKRNANVMVDAINAEDIADFPDANLAESLQRLPGVSID
                     RDNGEGRTITVRGLGSDFTRVRLNGVEALSTAGASIAGDNPNRSRGFDFNTFASELFN
                     SLKVQKTASAETEEGSLGATVDLQTGRPFDYSGRRIGLSFQDAYYENGETHNPRVAAL
                     LSDRWTINRLGQIGLIGSVAYNKRDQTIDSYQRQAGQSDYTYRQATFPGTPNIAAGTT
                     IQTRQGFAAPANTNCNGTLPGVVGVIPGVNITNISYCDALRGSNLAAYNLINSPIGST
                     LRNNNATGTAAGTTVAPGSLVRIPALPTLNQQDIGQERLGLTSGFQWRPTDRTTISVD
                     GVYSKLDQVSTNYQIVPVGLNRNNTQGTANPATYQLANATAGGTAAQRRALYAGCTAR
                     AETAIIAGVDCGQQIYGNTLVPGTTASYNPNNLDPFDYYNSTTSRGYVFDSLGLGMRN
                     ALIGRPGIRLIDAALSPTGQNADYLVLGNVDMRSAADEARFTTEFTQGTFDITHEFSD
                     TFRVHGIYGQSKSINESKGSLVEFSRLNSGSGAPGDGYFVYDARNGGDMPSLNFGFDV
                     ADPTKWDVIKGFSAIRYFERTVENTYEGAAFDLAWDFDENITLKAGFSRRVYTFFTTA
                     SQRESNRETLNPSLIEAGTTIQQVGAVHEFGQGLDLPEGTPTSFYAPNLDAFRSLFDF
                     ECNCVNKWGDWRISPKFNFAQTFDIEETDTSFFGQIDFLFQVFGRDVRGNVGVRQAKT
                     EVDSNGFTNRGRAINATNEYTNNLPSLNVAYEITDNMIVRLGIASVMSRPILGNLAPS
                     VTAFTVPNGLGANTGAAITIGNPKLNPFLATNYDLSFEWYFQPDALFSVAIFDKEISS
                     FPQTLVGEGTLSSILDTAGIAELRAEFEAVAADTTQTATARANAQNQVDYINANNSFS
                     IRQFRDAPGGYIRGVEVNYQQNFTFLPWYFQNLGIQANYTHLESELKYILTPQPLVTG
                     AGPFTGASPDAFNFTIFYEVPKFSARVSTAYRAEYVTQYPIASGTTDPGFSDSPLVND
                     FLGSDATLNVDLSMTYKLTDNVTLNFEALNLTNQTTNRWGYSDDPVVTNYGSTGRQFF
                     AGARMSF"
     gene            5862..6530
                     /locus_tag="VF633_09430"
     CDS             5862..6530
                     /locus_tag="VF633_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471321.1"
                     /translation="MRVETMPLAAPAVPSSSGVDIAEAHARLLRDPNLQFDRTGFVPP
                     EIPGWLHWVGDILKFIAPALKYVFWGGLIVIAGLILFAVAREILKLRGPRAKATKARQ
                     PPEPEWRPDVAAARDLLVQADALAADGLYAEAAHLLLLRSVEDIQKRQPKALRVSLTT
                     REIATLKAIPESARPAFTRIGSVVERSLFGAASVGAGDFADCRQAYEAFAIPDGWRAE
                     GMRT"
     gene            6527..7849
                     /locus_tag="VF633_09435"
     CDS             6527..7849
                     /locus_tag="VF633_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471322.1"
                     /translation="MSRTSRAVAGAFSPLTIIVLALIAVVAMAGLGVLSAYAPELKTG
                     DDGGSHALSNSSVGYAGIVKLLRSTGVPVTLSRGRVGMNGRDSLLITTPPFGVNPDQV
                     RDRARRSEEQGRDGMNVVGNAALTEITGGPLLIVLPKWNTLPEARHHGWVSTVGPYPV
                     DGVLEILPERFRSGLGLARRQSPGWIQMTRPDGHRTGRPVRIEALQTLRGKALAPAGN
                     AAPLWIPVLLDASGGVVLAMRRDTRIYVLSDPDLINTQGIKSLAGAETALAVLGLARA
                     EGSPVIFDLTLHGFQRTRSLLRLMLEPPLLGLTLLLAALAAFAGFQAVVRFGPAREAD
                     RTVALGKRALADNTAGLVRLARREHRMASPYARLIRAAVARAIGAPRTLSDTELDAFL
                     DRVGAMTGVTPEYSVLAANAAKARTPNDLMSVARDLHRWKQEMTRGRQ"
BASE COUNT         1441 a         2674 c         2357 g         1443 t
ORIGIN      
        1 gagcccacca gggtcgatcc caagctgatt gctggattga tcgcgtccga aaccgatgac
       61 tgggtcccgg tcattgcgcc catcggcgtg gccgacgacg gccagaccta taacgtcaac
      121 gccgacacgg tcgccggcgc catcgccggt tcgctgaacg caaaacgcat gctgctgctg
      181 accgacgtca agggtgtgct ggacaaaaac ggcgacatca tccgccagat gagcgtcgct
      241 gaggcgcaag gcctgatcga cgacggcact gcctcaggcg gcatgattcc caagctggaa
      301 accgccatgg cggccgtccg cgccggggtc gaagccgtgg tcatccttga tggtcgtcga
      361 ccccacgcca tgctggtcga gctgtttacc gaatacggcg ccgggacgct ggtgaagggg
      421 tgagcgccga cccggtcgaa tccaccgagc tgtccgctga cctgacgcac gtccggtcgt
      481 ggatattcga tctcgataac acgctgtatc cgccggagag ccagttcatg cggctggtcg
      541 agaaacgcat caacgactac gtggtgcgca cctcgggcct gcccggtccc gaggccctga
      601 tcgttcaaaa gggatatctg cgcgagtacg gcacctcgct ggccgggctg atggcgcact
      661 acaggatcga cccgcacgat ttcctcgccg aggtgcatga tgtccccttg gacgtattga
      721 cgcccgacgc gggtctgcgg gccggcctcg agcggctcaa aggaccgcgc ctcgtattca
      781 ccaacggttc tgccggccac gcaaagcggg tgctggaccg ccttgccctg agcgaccttt
      841 ttgacggagt gttcgcgctg gaagacgccg acctgattcc caagccggat ccgcgcacct
      901 ttgcccggat gactgaacgg tttggggtga gcgccgcttc ggcagccttc ttcgaggaca
      961 cggcccgcaa cctgaaaccg gccaaggcca tcggcatgac cacggttctg gtgggtgtcg
     1021 aagctgtcgc ctccccggac gcggcctcgg ccgattatat tgatttccgc gccgccgccc
     1081 tcggtccctt cctggccaca gcccatcttc acgagacgtg agacacgaat gaccctcgac
     1141 cgcagtcagc ttgcaaccgt catcgaacag gcctgggacg atcgcgccga gctatcgatc
     1201 aacacccacg gcgaggttcg cgatgcggtc gaacagtcgc tcgccctgct ggattcgggg
     1261 gaggcacggg ttgcctcgcg cggcgacgat ggcgtgtgga caacacatca gtggctcaag
     1321 aaggcagtgc tgctgtcgtt tcgcatcaac gagaacaaga tcatgcgggc tggggagcat
     1381 ggggtcccga gctcgacaag cggagcgccc ggcgtcggtc ctttctggga caaggtgccc
     1441 aacaagttcg gcgactggac tgccgcagat tatcaaaagg caggcttccg ttccgtgccc
     1501 ggagcgatcg tccgacaggg cgctcatatc gccaggaatg tcgttttgat gccttcgttc
     1561 gtgaacatcg gcgctttcgt cgacgagggt tcgatggtcg atgcatgggc caccgtcgga
     1621 tcctgcgccc agatcggcaa gaacgtccac ctgtcaggtg gggcgggcat cggcggcgtg
     1681 cttgagcccc tgcaagccaa tccgaccatc atcgaggacg gctgtttcat cggtgcccgg
     1741 gcggaggtcg ccgagggcgt gatcgtgcgc gaaggcgcgg tccttgccat gggcgtctat
     1801 ctgtccggct ccaccaggat cgtcgaccgc gccaccggtg aagtgttccg tggcgaggtc
     1861 ccggcctaca gcgtcgtagt gcctggcgcc atgccggacc cgaaaggcgg gccgtcgctg
     1921 tattgcgccg tcatcgtcaa gcgggtcgac gctcaaacgc gcgccaagac cggcgttaat
     1981 gaactcctgc gagactgaat ctgtcgctgc aatgcgcccg gcctccacaa cggcagcagt
     2041 ctcgagcctg cgcgctcgcg acgagcgtat ccactggtgc ttagtttgtc acacgaatga
     2101 aagtttctcc gctcccgctt gacaccggtg tcagctcacg cctttagttg cagggccgaa
     2161 acacggcgag attctgtcta gcccccacag aggggcgggc cagtcctggg aggagatcac
     2221 aatgaccacg ctccgcgtgc gcgctctgca atgcgcatcc actaccgctc ttgttgtcgg
     2281 cctgctgtcc gcgcagccgg tagctgccca gaccgcccct tccgagcccc aggccgggtc
     2341 ctccgatgaa gctgcccagg tcgatgaagt cgtggttacc ggcatccggg gcgcgctgcg
     2401 cagcgccctg aacaccaagc gcaacgccaa cgtgatggtc gatgcgatca acgccgaaga
     2461 tatcgccgac ttccccgacg ccaacctcgc cgagtccctg cagcgcctgc cgggcgtctc
     2521 gatcgaccgc gacaatggtg aaggccggac catcaccgtc cgcggcctcg gcagcgactt
     2581 tacccgcgtg cgtctgaacg gcgtcgaggc tctgtccacc gccggcgctt cgatcgccgg
     2641 ggataatccc aaccgctcgc gtggcttcga cttcaacacc tttgcgtccg agctgttcaa
     2701 ctcgctgaaa gtgcagaaga ccgcctcggc cgaaaccgaa gaagggtcgc ttggcgcgac
     2761 cgtcgatctg cagacgggcc gtccgttcga ctacagcggc cgtcgcatcg gcctgtcgtt
     2821 ccaggacgcc tattacgaga acggcgaaac ccacaatccg cgcgtcgccg cgctgctgtc
     2881 cgaccgctgg accatcaacc gtctgggcca gatcggcctc atcggctcgg tcgcctacaa
     2941 caagcgtgac cagacgatcg acagctacca gcgtcaagcc ggtcagtctg actacaccta
     3001 tcgtcaggcc actttccccg ggacgcccaa tatcgcagcg ggcaccacga tccaaacccg
     3061 tcagggcttt gcggctccag caaacactaa ctgtaacggt acgctcccgg gcgtggtcgg
     3121 cgtcattccc ggcgtcaaca tcaccaacat ctcgtattgc gacgcgctgc gcggttcgaa
     3181 ccttgcggcg tacaacctga tcaacagccc gatcggttcg acgttgcgca acaacaacgc
     3241 gacgggcacc gctgcaggta cgacggtggc gccgggctca ctggtccgca tccctgcgct
     3301 gccgactctg aatcagcaag atatcggcca ggagcgtctt ggcctgacga gcgggttcca
     3361 gtggcgcccc acggatcgta cgaccatcag cgttgacggc gtctattcca agctcgacca
     3421 ggtctccacc aactaccaga tcgtcccggt gggcctgaac cgaaacaata cccagggcac
     3481 ggccaacccg gcgacgtatc aactggccaa cgctacggct ggcggcaccg cagcccagcg
     3541 ccgggcgctg tacgcgggct gcacggcgcg ggccgaaacc gccatcatcg ccggtgtgga
     3601 ttgtggccag caaatctacg gcaacaccct ggttccgggc acgaccgcca gctacaaccc
     3661 gaacaacctc gatcccttcg actattataa cagcacgact tcgcgcggtt acgtgttcga
     3721 ctccctgggt ctgggcatgc gtaacgccct gatcggtcgc cccggcatcc gtctgattga
     3781 cgccgccctg agcccgaccg gtcaaaacgc cgattatctg gtgctcggca acgtcgatat
     3841 gcgctcggcg gccgatgaag ctcgtttcac cacggagttc acccagggca cgttcgatat
     3901 cacgcacgag ttcagcgaca cattccgtgt ccacggcatc tacggtcaaa gcaagtcgat
     3961 caacgaatcc aagggttcgc tggtcgagtt cagccgcctg aactcgggct ccggcgcgcc
     4021 cggagacggc tatttcgtct atgacgcccg caacggcggc gacatgcctt cgctgaactt
     4081 cggcttcgac gtcgccgacc cgaccaagtg ggacgtgatc aagggcttct cggcgatccg
     4141 ctatttcgag cggacggtcg agaacaccta cgaaggcgcc gccttcgatc tggcctggga
     4201 cttcgacgaa aacatcacgt tgaaggcagg cttttcacgt cgggtctata ccttcttcac
     4261 cacggcctcg cagcgcgagt cgaaccgcga aaccttgaac ccgagcctga tcgaagccgg
     4321 gacgacgatc cagcaggtcg gcgccgtgca cgagtttggc caaggtctgg acctgcccga
     4381 aggcacgccg acaagcttct acgccccgaa cctagacgcc ttccggagcc tgtttgactt
     4441 cgagtgcaac tgtgtcaaca agtggggcga ctggcgcatc tcgccgaagt tcaacttcgc
     4501 ccagacgttc gatattgagg aaaccgacac cagcttcttc ggccagatcg acttcctgtt
     4561 ccaggtcttc ggtcgtgacg ttcgcggtaa cgtcggtgtt cgtcaggcca agaccgaggt
     4621 ggactccaac ggcttcacca accgcggtcg ggctatcaac gccactaatg aatacaccaa
     4681 caacctgccg tcgctgaacg tggcctacga gattacggac aacatgatcg tgcgtctcgg
     4741 catcgccagc gtgatgtcgc gtccgatcct cggcaacctg gcgcctagcg tcaccgcctt
     4801 cacggtgccc aatggcctcg gcgccaacac cggcgccgcc atcactatcg gcaaccccaa
     4861 actgaacccg ttccttgcaa cgaactacga cctcagcttc gagtggtatt tccaacccga
     4921 cgccctgttc tcggttgcca tcttcgacaa ggaaatctcg tccttcccac agacgctggt
     4981 tggcgaaggc acgctgtcgt cgatcctcga caccgcaggc atcgccgaac ttcgcgccga
     5041 gttcgaagcc gtcgccgccg atacgaccca gaccgcaacg gcgcgtgcca atgcccagaa
     5101 ccaggtcgat tatatcaacg cgaacaactc gttctctatc cgccagttcc gcgacgcgcc
     5161 cggcggctac atccgcggcg tcgaggtcaa ctatcagcag aacttcacct tcctgccctg
     5221 gtacttccag aacctcggca tccaggccaa ctacacccac ctggagtcgg agctgaagta
     5281 catcctgacg cctcagcctc tggtgaccgg cgccggaccc ttcaccggcg cctcgcctga
     5341 cgcgttcaac ttcaccatct tctatgaagt accgaagttc tccgcccgcg tatcgacggc
     5401 ctaccgtgcc gaatacgtga cccagtaccc gatcgcctcg ggcaccacgg atcccggctt
     5461 ctctgactcc ccgctggtca acgacttcct cggttccgat gccacgctga acgtcgacct
     5521 ctccatgacc tacaagctca cggacaacgt cacattgaac ttcgaggccc tgaacctgac
     5581 caaccagacc accaaccgct ggggctactc ggacgatccc gttgtgacca actacggttc
     5641 gaccggccgc cagttcttcg ccggcgccag aatgagcttc taggacgatc caacgctatc
     5701 ctcgtgtttt cgcagccgga gcgtctcagg acgctccggc ttttttcgag atcgcggcaa
     5761 agacctgaaa ccgtcatcga atacgggcat aaaaccacgt ccggttgact ggactcacgg
     5821 tcacgctatt gctggatgcg tcaggggata cggatcagcg tatgcgggtt gagaccatgc
     5881 cgcttgcggc gccagccgtc ccgtcttcgt cgggcgtcga catcgccgag gctcacgccc
     5941 ggctgctccg cgatccgaat ctccagttcg accgcaccgg cttcgttccg cccgaaattc
     6001 ctggctggct tcactgggtc ggcgacatcc tgaaattcat cgcacctgcc ttgaaatacg
     6061 tcttctgggg cggattgatc gtgattgccg gtttgatcct gtttgccgtc gcgcgagaga
     6121 ttctgaagtt gcgcgggcca agggccaagg cgacgaaagc ccgacaaccg cctgagccgg
     6181 aatggcgtcc cgacgtcgcc gcagcccgcg atttgctggt ccaggctgac gcgctcgccg
     6241 ctgacggtct gtacgccgag gccgcgcacc tgctgctgtt acgtagcgtc gaagatatcc
     6301 agaagcgtca gccgaaggcg ctccgcgtct ccctgaccac gcgcgaaatc gcgacgctga
     6361 aagccatccc tgagagcgcc cgaccggcgt ttacccgcat cggcagcgtc gtggaacgca
     6421 gcctgttcgg cgcggcgtcg gtcggcgctg gcgacttcgc agactgccgt caggcctatg
     6481 aagccttcgc aattcctgac ggctggcgag ccgaagggat gcggacatga gccggacttc
     6541 gcgcgccgtt gcgggcgcct tctccccgct gaccatcatc gtcctggccc tcatcgcggt
     6601 ggtggccatg gccggtctcg gtgtgctgtc tgcgtatgcg ccggaactca agaccggcga
     6661 tgacggtggg agccacgcgc tgtccaactc atcggtaggc tacgccggga tcgtcaaact
     6721 tctgcgttcc acgggtgttc cggtgaccct cagccgaggg cgggtgggca tgaacgggcg
     6781 cgatagcctg ttgatcacga caccgccctt tggggtcaat ccggatcagg ttcgagaccg
     6841 tgcccgtcgc tcggaggagc aaggtcgtga cggcatgaac gtcgtgggca acgctgcgct
     6901 gacagaaatc accggcggcc cactcctgat cgtgctgcct aaatggaaca cccttccgga
     6961 agctcgccat catggatggg tctcgactgt gggtccttat cccgttgacg gcgtgctcga
     7021 aatcctgccc gaacgctttc gttccgggct tgggctcgcg cgccggcagt ctccaggctg
     7081 gatccagatg acgcggcccg acggccatcg caccggccgc ccggtgcgta tcgaggccct
     7141 gcagacgttg agaggcaagg cgttggctcc agccggaaac gccgccccgc tatggatccc
     7201 cgtgctgctg gatgcatccg gcggagtcgt actcgccatg agacgtgaca cgcgaatcta
     7261 cgtgctgtcc gatcccgacc tgatcaatac gcagggtatc aagtcgttgg ctggagccga
     7321 gaccgccctg gctgtgctcg gccttgcgcg cgccgagggc tcgccggtaa tcttcgatct
     7381 gaccctgcac ggcttccagc ggacgcgcag cttgctgcgg ctgatgctcg aaccgccgtt
     7441 gctggggctg acactcctgc tggctgcctt ggctgccttt gccggctttc aggctgtggt
     7501 tcgcttcggc cccgcacgcg aggcggatcg caccgtagct ctgggcaagc gcgccctcgc
     7561 cgacaacacc gccggtctgg tgcggctggc acggcgcgag caccggatgg cttcgcccta
     7621 tgcccgcttg atccgcgccg ccgtcgctcg cgccatcggg gcgccgcgta cgctgtcgga
     7681 taccgagctc gacgccttcc tggatcgcgt cggagccatg accggcgtga cgcccgaata
     7741 ttccgttctg gccgcgaacg ccgccaaggc ccggaccccc aatgatctga tgtcggtcgc
     7801 ccgtgacctg catcgctgga aacaggagat gacccgtgga cgtcaatgag gtgaccgcaa
     7861 tcgccgcgcg gatccgcgcc gagatcgccc gcgcagtggt gggtcaggac ggcgc
//
LOCUS       DASVTM010000348         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_939052,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000348 DASVTM010000000
VERSION     DASVTM010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939052"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VF633_09440"
     CDS             <1..472
                     /locus_tag="VF633_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX8471323.1"
                     /translation="GLTDMGALIDIYVAQPFWLWLAIGVVLLAIEASISTEWLLWPAV
                     SAGVVAVICALGLRFGFAGEAGIFAVLTLASTLASRKLVQRVNPDEYPDINDRDDRLV
                     GQRARVVEAFIEGRGRVFISGAEWPAEIEGAAPVVGDSVVVERSTGSGLKVRLA"
     gene            complement(477..938)
                     /gene="rnhA"
                     /locus_tag="VF633_09445"
     CDS             complement(477..938)
                     /gene="rnhA"
                     /locus_tag="VF633_09445"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX8471324.1"
                     /translation="MNAPANHVIIHTDGACKGNPGPGGWGAILEAAGKVKEMSGGERL
                     TTNNRMELMAAIMALEALTRPCKIDLHTDSKYVLDGITKWISGWKSRGWLTADKKPVK
                     NDDLWKRLDAARIRHEVKWHWVKGHAGHPLNERADQLANRGIVEMKAGARP"
     gene            1043..1261
                     /locus_tag="VF633_09450"
     CDS             1043..1261
                     /locus_tag="VF633_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8471325.1"
                     /translation="MGSFSIWHWAIVAVIVLLLFGGRGKLSGIMGDAAKGIKAFKEGL
                     KDDDKKDGPSEGVSSLPRTDAEKEELKR"
     gene            1299..1961
                     /gene="tatB"
                     /locus_tag="VF633_09455"
     CDS             1299..1961
                     /gene="tatB"
                     /locus_tag="VF633_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX8471326.1"
                     /translation="MGPGIGGSEWFIIGLVALLVVGPERLPGLLRQLGKMVAKARGMA
                     NEFRASFDEMARQSELDELRKEVEALRTVQSPVRLGADADAAFKGIKDEFDKPLFAAA
                     TPASGAPVVPESVAEPVMTPTASEWPDGEPVMMPLATPSLTEPEAEPVVAATPVKRAA
                     PRKSAANASLTKATTPKTSVSKVPGANALAKTGTAAAAKSGTATKTTAKARAPRNSKL
                     DL"
     gene            1958..>2437
                     /locus_tag="VF633_09460"
     CDS             1958..>2437
                     /locus_tag="VF633_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX8471327.1"
                     /translation="MTYERNEAEIEESRAPLMDHLIELRGRLMICVIGFVIGFLICFY
                     FASPIQIFLIKPYQAATAIHAAAAAHGGHGNPIELLEVTSGLKPIPKDGESAVQLIAT
                     APLEQLFSKMKIAGFGAIVLAFPIIAWQLYRFVAPGLYRNERGAFLPFLLAAPALFIL
                     "
BASE COUNT          410 a          762 c          785 g          480 t
ORIGIN      
        1 cggcctgacc gacatgggcg ccctgatcga catctatgtg gcccagccgt tctggctctg
       61 gcttgccatc ggggttgtcc tgttggccat cgaggccagc atttcgacag agtggctgct
      121 ttggcccgca gtctcggcgg gggtggtggc cgtgatttgc gccctcggct tgcggttcgg
      181 gtttgcaggg gaggcgggaa tattcgccgt gctgaccctc gcatccacgc tcgcgtccag
      241 aaagctggtc cagcgggtca atcccgacga atatccggac atcaatgacc gcgacgatcg
      301 acttgtcggg cagcgcgccc gggtggtcga ggcgttcatc gaaggccgtg ggcgggtctt
      361 catttcgggc gcagaatggc ctgcggagat agaaggcgcg gctcctgtcg tcggtgacag
      421 cgtggtggtg gagcgctcga cggggtctgg tctcaaggtc cgtttggcct aagggcttaa
      481 ggccgagcgc cagccttcat ctcgacgatg ccgcgattgg ccaactggtc ggcgcgttcg
      541 ttgagggggt ggccggcgtg acctttgacc caatgccatt tgacctcgtg cctgatgcgg
      601 gcggcgtcga gccgcttcca caggtcgtcg ttcttgaccg gtttcttgtc cgccgtcagc
      661 cagccgcgcg acttccagcc cgagatccat ttggtgatcc cgtccagcac gtatttgctg
      721 tcggtgtgca ggtcgatctt gcaaggccgg gtcagggctt ccagcgccat gatcgccgcc
      781 atcaactcca tgcgattgtt ggtggtcagg cgctcgcccc ccgacatctc cttgacctta
      841 cccgcggctt caaggatcgc accccagcct ccggggcccg gattgccttt gcaggcccca
      901 tcagtgtgga tgatgacgtg gtttgcaggc gcgttcatgc gggtgtgact acagcgtcga
      961 ttgccgcgcc gccaagcaat agcctatatg acggctgaga tttgttacgg gaccgaatcc
     1021 ggtcccttga ggagacgcca ccgtgggcag ctttagcatc tggcattggg ccatcgtcgc
     1081 ggtcatcgtt ctgctgctgt ttggcggccg cggtaaactg tcgggcatca tgggcgacgc
     1141 cgcgaaaggc atcaaagcct tcaaggaagg cctgaaggac gacgacaaga aagacggccc
     1201 gtccgaaggc gtgtcgagcc tgccgcgcac cgacgcggaa aaagaagagt tgaagcgcta
     1261 gtcgctactg accggaaaga gcctgcagca tgggcggtct gggtcccggt atcgggggca
     1321 gtgagtggtt tatcatcggc ctggtggcgc ttttggtcgt aggaccggag cgtctgcccg
     1381 gcctgttgcg ccaactcggc aagatggtcg ccaaggcgcg cggcatggcc aacgaatttc
     1441 gcgccagctt tgacgaaatg gcgcgtcagt ccgagctgga cgaactccgc aaggaggtcg
     1501 aggccctgcg gactgtgcag tcacccgtcc ggttaggcgc cgatgcagac gccgccttca
     1561 agggcatcaa ggacgagttc gacaagccgt tgtttgccgc tgccacaccc gcgtcaggtg
     1621 cgccggtcgt gccggagagc gtcgctgaac ctgtgatgac gccgaccgcg tccgaatggc
     1681 ccgacggcga gccggtgatg atgccgctgg ccacgccttc gctgacggag cctgaggctg
     1741 agcctgttgt cgcggcaacg cccgtcaaac gcgccgctcc ccgaaaatct gcggctaacg
     1801 cgtctttgac gaaagccacc acgccgaaga cttcggtctc caaggtccct ggcgccaacg
     1861 ctctggcgaa gacaggcaca gccgccgctg ccaaatccgg gaccgccacc aagaccacgg
     1921 cgaaggcccg cgcgccgcgt aacagcaagc tcgatctatg acctacgaac gtaacgaggc
     1981 cgagatcgag gagtcgcgcg ctcccctgat ggatcatttg atcgagctgc gcggccggct
     2041 gatgatctgt gtgatcggtt tcgtgatcgg cttcctgatc tgcttctact tcgcctcgcc
     2101 gatacagatc ttcctgatca agccttatca ggccgcgacc gccattcacg ccgcggcggc
     2161 ggcacacggc ggtcacggca atccgatcga actgctggag gtcacgtcgg gcctcaagcc
     2221 gattcccaag gacggcgagt cggcggttca gctcatcgcg accgcaccgc tcgaacagct
     2281 gttcagcaag atgaagatcg ccggcttcgg ggccatcgtt ctggctttcc cgatcatcgc
     2341 ctggcagctg tatcgctttg tcgcgcccgg cctttaccgc aatgaacgag gggccttcct
     2401 gccgttcctg ctcgcagcgc ccgcgctgtt catcctc
//
LOCUS       DASVTM010000349         4788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_34284, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000349 DASVTM010000000
VERSION     DASVTM010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34284"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF633_09465"
     CDS             complement(<1..224)
                     /locus_tag="VF633_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX8471328.1"
                     /translation="MTQDDFERLQTLLATRAGFRLTRDRIHLAEHRLAPVARREGFDS
                     VDALLAALWARPVASLAWAVIETMLNGETW"
     gene            complement(244..615)
                     /locus_tag="VF633_09470"
     CDS             complement(244..615)
                     /locus_tag="VF633_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471329.1"
                     /translation="MDARTCLIVDDSRIIRKVARRIVEGLGFEVDEAADGAEALTYCA
                     SVMPDIVLLDWNMPVMDGLTFLRRLRAQPGGDAPKVLFCTIETAPERIAEGLAAGADD
                     YVMKPFDGEILQSKLAEVGAV"
     gene            complement(702..1352)
                     /locus_tag="VF633_09475"
     CDS             complement(702..1352)
                     /locus_tag="VF633_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEX8471330.1"
                     /translation="MTDDTSALPSGAELATWVAAKLCHDFVSPAGAINSGLDLLNDPS
                     AADMRDDALGLITQSATKLLSLIYFCRVAFGAATTSERFTGEELRGLVSGMIEGGRAT
                     MDWRVSEADFNKPASRALLNLAYLTAGALPSGGEAVITSHRDGDMLVLTGLAEGARAR
                     LKPEAIVGLSGQRLTEGLTGQWIQPYWLWLTAHEAGGSLSVVAEEGRVALVARMPV"
     gene            1458..2240
                     /gene="cysQ"
                     /locus_tag="VF633_09480"
     CDS             1458..2240
                     /gene="cysQ"
                     /locus_tag="VF633_09480"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX8471331.1"
                     /translation="MSKSLTQELASGALHDAIADIAEDAARVILPYWRAGAVVTTKSD
                     DSPVTLADQQAEALILERLNALYPGVQTVAEEAVAADGAPEKADELFWLIDPLDGTKG
                     FIAGKESFTVNIALIQNGAPIAGVVSAPATSTTWRLGPGGGAQRRRFGEAWVAIKVRN
                     RPDEGIALLSHSVSDEEASRLAARHGCTQWQGTDSSLKFCLIAEGRFDAYPRTGPTHE
                     WDTAAGQAVLEAAGGRVLGEDGQRMVYGKPGFRNGAFVAMGG"
     gene            complement(2264..3505)
                     /locus_tag="VF633_09485"
     CDS             complement(2264..3505)
                     /locus_tag="VF633_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471332.1"
                     /translation="MFEFGRDLRRLFAQARESEDLSWLELIGVDLLAVEARQQCTDAG
                     RISCVKPHPAWMRGSALWREHARRSGSADSVRKALGAALDAARHAKSPDEVARAAVET
                     AQGLLLRFDLNGGREVLDQALEAVPGTTGCRSATISLISAIHARIKSRLARFENDSGA
                     LMGAAALLDAAIHDMDRTRAGNVDDIRMDRAALALEAGVTRRDPHLLDQAGRELLRLV
                     ANASPDYRPLTRARALTLCGAGLSALGALADSEPAREQGKAMFEAAADQFTPDHSPLD
                     WVVIQIVRGATPGAVSLAALRQADSLTAGQNLVVGAMARDMLIEGEVAAAVSTGDVPR
                     LTVSETAARRRLSDNPSELNWAVEQMAMVRIARARALLTGSDASATGLALLEAAEVAY
                     ERGMPGLADRAKALLGVRLNA"
     gene            complement(3638..3985)
                     /locus_tag="VF633_09490"
     CDS             complement(3638..3985)
                     /locus_tag="VF633_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471333.1"
                     /translation="MFAIWIAALLTVNAQTARPAVALVSYDETVRCAGVTQAASELEG
                     GETAEGRALSDAALYWSLAATQAAEASNRDAKVSDADQNRARIRAVRQLNAGNADARA
                     ALLRCRARTPDLG"
     gene            complement(4043..>4788)
                     /locus_tag="VF633_09495"
     CDS             complement(4043..>4788)
                     /locus_tag="VF633_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471334.1"
                     /translation="ALQTERWLPIDVVLRADSSLGVGSQTGRSMYYAQSWALTHWLLA
                     DTARIRNLSAYVRAVNGGQDPVEVWQAVFAMTPDQLTAQLRTHVRSRLSYAKLDIPPI
                     DPVITVTTLSPAADVVILPAINARSWNTAEVDGPALLATLRTAAARFPDDPLALVALG
                     RAEMRWGNATAAETALTHALDRQGDNVEGLILMADLTTERGAEAIDEAERTRQWDLAR
                     DFLARALQADPTDQRVYSAQARARRYIGD"
BASE COUNT          818 a         1537 c         1660 g          773 t
ORIGIN      
        1 aaccaggttt cgccgttcag catggtctcg ataacggccc aggcgagcga agcgacggga
       61 cgggcccaga gcgcagcgag cagggcgtcg acgctgtcga atccctcgcg acgagccacg
      121 ggcgcgagcc gatgttcggc caaatggatt cggtcgcggg tcaggcggaa gccggcccgg
      181 gttgccagca acgtctggag gcgttcgaaa tcgtcctggg tcatgcgtga cttgaaggcg
      241 tcgtcagacg gcccccacct ccgcaagctt ggactgcagg atttcaccgt cgaaaggctt
      301 catgacgtag tcgtcggcgc cggcggccag accttcggcg atacgctcag gggcggtctc
      361 gatggtgcag aacaggacct ttggggcatc gccgcctggt tgcgcgcgca ggcgccgcag
      421 gaacgtcaat ccgtccatga cgggcatgtt ccagtccagc aggacgatgt ccggcatgac
      481 cgaagcgcaa taggttaggg cctcggcgcc gtcggcggcc tcgtcgacct cgaagcccaa
      541 gccttccacg atgcgccgcg cgaccttccg gatgatccgg ctgtcgtcga cgataaggca
      601 ggttctggcg tccaaagtcc gacttctccg gcagggaaag gcgtccgtgc gtcgcgagca
      661 tcatcgccct gtggcagtta acgaggcgtt gggaaacggt attaaacggg catccgggcg
      721 accagcgcaa cccgtccttc ttcagcgacg acgctgaggc ttccgccggc ctcgtgagcc
      781 gtaagccaaa gccagtaggg ttgaatccac tggcctgtca ggccctccgt caatcgctgg
      841 cccgagaggc cgacaatggc ctcgggcttc agccgtgcgc gtgcgccttc ggccagtcct
      901 gtcaacacca gcatgtcgcc gtcccggtgg ctggtgatca cggcctcgcc tcctgatggg
      961 agcgcgccgg cggtgagata ggccaggttc agcaaggcgc gggaggcggg cttgttgaag
     1021 tcggcttcgg aaacgcgcca gtccattgtg gctcttcctc cttcgatcat gcccgagaca
     1081 aggccgcgca attcctctcc cgtgaaccgc tcgctggtcg tcgcggcgcc aaaggcgacg
     1141 cggcagaaat agatcagcga cagcaatttc gtcgcgcttt gtgtgatcag gccgagcgca
     1201 tcgtcgcgca tgtcggcggc ggaaggatca ttgagcaggt ccaggcccga gttgatcgcg
     1261 ccggcaggac ttacgaagtc gtggcaaagc ttggctgcga cccaggttgc cagttcggcg
     1321 ccggacggga gggcggacgt gtcgtcggtc atgggggctc ttttgcgcgg gcgcgaatgt
     1381 cgttctggga cgtgtgcacg caaactgagg tgatttgcca gttcgcgaaa cgggtcggat
     1441 cggctatcga cccggccatg agcaaatcac tgacgcagga actcgcttcg ggcgccctgc
     1501 atgacgccat cgcagacatc gcggaggacg ccgcgcgcgt gatcctgcct tactggcgcg
     1561 cgggcgccgt cgtgaccacc aagtcggacg acagccccgt gacgctggcc gaccagcagg
     1621 ccgaagcact gattctggag cggctgaacg cgctctatcc cggagtgcag accgtcgccg
     1681 aagaagccgt tgccgccgac ggcgcgcccg agaaagcaga cgaacttttc tggctgatcg
     1741 atccgctgga cggcaccaag gggtttatcg caggcaagga gagcttcacc gtcaacatag
     1801 ccctgatcca gaatggagcg cccattgccg gcgtggtcag tgcgcccgcg acatcgacga
     1861 catggcgact gggtccgggc ggcggcgccc agcgccggcg attcggggag gcgtgggtcg
     1921 cgatcaaggt ccgaaaccgc ccggatgagg gaatcgccct gttgagccac agcgtgagcg
     1981 acgaagaggc gagccgtctg gccgcccgtc atggctgcac ccaatggcag gggaccgatt
     2041 cgtcgctgaa attctgcctg atcgccgagg gccgtttcga cgcctatccc cgcacgggcc
     2101 cgacccacga gtgggacacg gcggcgggtc aggcggtgct ggaagcggct ggcggtcgcg
     2161 tgctgggcga agacggccaa cggatggtct acggcaagcc gggcttcagg aacggggcgt
     2221 tcgtggccat gggcggctga tcagtcttcg aactcaccga ctgtcaggcg ttcaggcgga
     2281 caccgagcag ggccttggcc cgatcggcca gtccgggcat gccgcgctcg tacgcgacct
     2341 ctgccgcctc gagcaatgcg aggcccgtag cgctggcgtc tgaaccagtc agaagagcgc
     2401 gggcgcgggc gatacggacc atcgccattt gctcaacggc ccagttcagc tcggacgggt
     2461 tatcggacag acgtctgcgg gctgcggtct cggaaaccgt tagccgcggg acgtcgcctg
     2521 tcgagactgc ggctgcgacc tcgccctcga tgagcatgtc gcgagccata gctccgacga
     2581 cgagattctg gccagcggtg agactgtcgg cctgacgcag ggccgcgagg gagacagcgc
     2641 cgggcgtcgc cccgcgaacg atctggatta caacccagtc gagcgggctg tggtcgggcg
     2701 taaactgatc tgcagcggcc tcgaacatgg cctttccctg ttcgcgcgcc ggttcgctgt
     2761 cggccagcgc accgagggcc gagagaccgg ctccgcacag ggtgagggcg cgggcgcggg
     2821 tcagggggcg gtaatcgggg gatgcgttcg cgaccagtcg aagcagttcg cgcccggcct
     2881 gatccagaag atgcgggtcg cgacgcgtga cgccggcttc cagcgccagc gcagcgcgat
     2941 ccatgcggat atcgtcgaca ttgcccgcgc gggtccggtc catgtcgtgg atggcggcgt
     3001 cgagcagggc ggccgcgccc ataagagcgc cggagtcgtt ctcgaaacgg gcaaggcgcg
     3061 acttgatccg cgcgtggatg gcggaaatga gggagatggt tgctgaacgg cagccggtcg
     3121 tcccgggtac ggcctcaagc gcctggtcga gaacttcgcg gccgccgttg aggtcgaagc
     3181 gtaacagcag gccctgcgcc gtctctacgg cagcgcgtgc gacctcgtcg ggagatttcg
     3241 catggcgggc ggcgtccagt gcggcgccca gagccttgcg gacgctgtcc gccgacccgg
     3301 aacgacgggc gtgctcgcgc caaagcgctg agccccgcat ccacgccgga tgaggcttta
     3361 cacaggagat gcgtccggca tcggtgcact gctgacgggc ttccacggcc agaagatcca
     3421 cgccgatcag ctccagccaa ctcagatcct cgctttcgcg cgcctgtgcg aacagccttc
     3481 tcaagtccct gccgaattcg aacatacgcc tcgtcagcct ctcggtcgtc ccgcttcggg
     3541 accgcaaagg cgatcccggg gacgcccacg caaacgcagg gccggttcga gggttcagct
     3601 cagctttccg gatggggggc tgaaccgaca tacaggatca gcccaggtcg ggcgtgcgcg
     3661 cccggcaccg cagcagggct gccctggcgt ccgcattccc ggcattgagc tgccgcactg
     3721 cccggatacg ggcgcggttc tggtcggcgt ctgagacctt ggcgtcccgg ttggaggcct
     3781 ccgccgcctg ggtggccgcc aaggaccaat atagtgcggc atcggacaag gccctgccct
     3841 cggctgtttc accgccttcg agttcggaag ccgcctgggt cacgccggca cagcgaaccg
     3901 tctcgtcata ggagacgagc gccaccgccg gccgggcggt ctgggcgttg acggtcagca
     3961 gggcggcgat ccagatcgcg aacatgacgc cagagaacca cgggagaaag gcgtagggaa
     4021 ggccggtcgg ctgagtacgt gttcagtcgc ctatataccg gcgcgcgcga gcctgggcgc
     4081 tgtaaacccg ctggtcagtg ggatcggcct gcagcgcgcg ggccaggaaa tccctcgcca
     4141 ggtcccactg ccgcgtccgc tcggcctcgt cgatcgcttc ggcgccccgc tccgtggtca
     4201 agtccgccat caggatgagg ccctcgacgt tgtcgccctg ccggtcgagc gcgtgggtca
     4261 gggccgtctc cgcagcggtc gcatttcccc accgcatttc cgcccggccg agggcgacaa
     4321 gggccagagg atcgtcgggg aagcgggcgg cggccgtccg cagcgtggcc agaagcgcgg
     4381 gtccgtcgac ctccgccgta ttccacgacc tagcgttgat cgccggcaga atgaccacat
     4441 cagccgcagg cgacaaggtc gtcactgtga tgaccgggtc gatcggagga atgtcgagct
     4501 tggcgtagga gagacggctt cggacgtggg tgcgaagctg cgccgtcagc tggtcaggcg
     4561 tcattgcgaa gaccgcctgc cagacctcca cggggtcctg accaccgttg actgccctga
     4621 cgtaggcgga cagattccgt atccgggcag tatcggccag cagccagtgg gtcagggccc
     4681 aggactgagc gtaatacatg ctccgtcctg tctggctgcc gacgcccagc gagctgtctg
     4741 cccgcagcac gacgtcaatg ggcaaccagc gctcggtctg cagcgctt
//
LOCUS       DASVTM010000350         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_780097,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000350 DASVTM010000000
VERSION     DASVTM010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780097"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..719
                     /locus_tag="VF633_09500"
     CDS             90..719
                     /locus_tag="VF633_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX8471335.1"
                     /translation="MDYAAARKVMVDSQVRVNDVTDRSLQAVLLSVPRERFCAPERAF
                     AAYAEIETAITPTRKLMMARDVAKLIQALDARAGETALVVAGPYAAAVLAGLGLTVTA
                     QESDAAVFDIAGEALEDAGVTTAIAPMTEPAGGEYDVIISEMATPVRPEAWLQTLKVG
                     GRLAVVERSGPAGKAVLYVRGREGFSRRELFDSSPQVMAEMSPPMIFAL"
     gene            856..2346
                     /locus_tag="VF633_09505"
     CDS             856..2346
                     /locus_tag="VF633_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX8471336.1"
                     /translation="MLKRSSVVAVALASVSWLAISAGNASAETLQEALALAYRTNPTL
                     LAQRANQRALDETIPQARSGYRPTIDVTAGASYTRTDIQGSGLGSGVTETDGGSLSIG
                     LNQTLYSGGRIGHSVTAAEANVLAGRENLRSVEQQVLASVIQAYVGVQRDMQVLQIRQ
                     ENLAVLRRQLDESNARFEVGEITRTDVAQSEARLAQSEADLANAQAQLSVSRATYAAV
                     VGQAPAGLVTPPALPGVPADFEQALDIGLVENPSVVAAGYSLQAAEARVAAARSAYLP
                     SAGLTASYGGSGDLSEFDLADRTSLTVGAQISVPLFTGGLNASRVAQALETANAAQIA
                     VEGERRGVLQDVSSAFAQVVSARATVTAGNEAVRAATVAAEGVRQEAQVGLRTTLDVL
                     NQELELRSAQVLLVNSVANEYIAQASLLAAMGRLEGPDLDPSIEAYDAAANTNAVRGR
                     GALPWDGVLEAIDRIAAPLVTPATDAPDAPIDTQLKSDIVKTAPRS"
     gene            2524..3105
                     /locus_tag="VF633_09510"
     CDS             2524..3105
                     /locus_tag="VF633_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX8471337.1"
                     /translation="MSEPTAQEPTMEEILASIRRIISEDDAPVETVAAPAPEPEPFPS
                     PVADAAPSAPEPVEPEEAEDDILELTDTYEAPAVESIGDLDVSTPEPEAPATWNPSPE
                     PEPAQSYDSLVGESAAASAASAFAGLHASLKKPEPAPAASNDLNFISGMTVEGMVAEM
                     LKPMLKNWLDSNLPGIVEAAVKAEVERIARTAG"
     gene            3216..>4315
                     /locus_tag="VF633_09515"
     CDS             3216..>4315
                     /locus_tag="VF633_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8471338.1"
                     /translation="MLDKTFDPKASEPRLYAQWEQSGAFAPTEGAAEAYSIVIPPPNV
                     TGSLHIGHALNNTLQDILTRYHRMKGKAALWLPGTDHAGIATQMVVERQLAAAGNMGR
                     REMGRDAFVEKVWEWKAESGGSIVQQLRRLGASCDWSRERFTLDEGLNAAVRKVFVQL
                     HKEGLIYRDKRLVNWDPQFQTAISDLEVEQREVEGFYWHFAYPLADGVTYEHPIAFDE
                     EGVATEYETRDYIVVATTRPETMLGDTGVAVHPGDERYASLVGKFVTLPIVGRRVPIV
                     ADDYADPTKGSGAVKITPAHDFNDFQVGKRAGLATINILDAFARITDADAPDIPTEYT
                     GQDRFAARKAIVARAEAEGWLKEIEKTKHVVP"
BASE COUNT          769 a         1451 c         1375 g          720 t
ORIGIN      
        1 gttcgctatc agatcggatt gtccgctgca acgcacggac ctataagcgc gtcagatttc
       61 gtctctaaac gcacaaggat tcgccgctga tggattacgc cgccgcgcgc aaggtcatgg
      121 tggactctca ggtccgggtg aacgacgtga ccgatcgcag ccttcaggcc gtgctgctga
      181 gcgtgccgcg cgagcgtttc tgcgccccgg aacgggcctt cgcggcctac gccgagatcg
      241 agaccgcgat cacaccgacc cgcaagctga tgatggcgcg ggacgtggcc aagctgatcc
      301 aggctcttga cgcccgggcc ggcgagacgg cgctcgtggt cgcgggtccc tacgctgctg
      361 ctgttctggc ggggctgggc ctgacagtca cggctcagga gtctgatgcg gcggtcttcg
      421 acattgccgg cgaagccctg gaagacgccg gcgtcacgac cgcgatcgcc ccgatgaccg
      481 agcctgccgg cggtgaatac gatgtcatca tcagcgagat ggcgactccc gtccggcccg
      541 aagcctggct gcagacgctc aaggtcggcg ggcggctggc agtcgtcgag cgctccggac
      601 ccgcaggcaa ggcggtcctc tatgtgcgcg gccgcgaagg cttttcgcgc cgtgaactgt
      661 tcgattccag ccctcaggtg atggccgaaa tgtcgccgcc gatgattttc gcactgtaga
      721 gtccattctt ccgacggaat gggctggcca ctgcgtgaaa aaaacttaac tggcgtggcg
      781 taggcggaat gcgctctacg cgagaacaag aaattcaggc cgcgctccac cgcgaacgcc
      841 tatcgggaat tggtcatgct gaaacgttcg tcggtcgtcg cggtcgccct cgcctctgtc
      901 tcgtggctcg ccatctcggc gggaaacgcc tctgccgaga cgctgcagga agcgctggcg
      961 ctggcctatc gcaccaaccc tacgttgctg gcccagcgcg ccaaccagcg agcgctcgac
     1021 gagaccattc ctcaggcgcg gtcgggctat cgtcccacca tcgatgtcac agccggcgcc
     1081 agctacaccc gcaccgatat tcagggcagc ggtctgggca gcggcgtaac tgaaaccgac
     1141 ggtggcagcc tgagcatcgg gctgaaccag acgctctaca gcggcggccg catcggtcat
     1201 tccgtcaccg cggccgaggc caatgtcctc gccggtcgtg agaacctgcg ctccgtcgaa
     1261 caacaggttc tggcctccgt tattcaggcc tatgtcggcg tccagcgcga catgcaggtc
     1321 ctgcagatcc gtcaggagaa ccttgccgtc ctgcgtcgcc agttggacga atccaacgcc
     1381 cgctttgaag tgggcgaaat cacgcggacg gacgtcgccc agtcggaggc ccggctggct
     1441 cagtccgaag ccgatctggc caatgcccag gcccagctgt cggtgtcgcg cgcgacctac
     1501 gccgctgtgg tgggccaggc tcccgccggt cttgtgactc cgccggcctt gccgggcgtg
     1561 cccgccgatt tcgaacaggc gctcgatata ggtctggtcg agaacccttc tgtcgtggcg
     1621 gccggctaca gccttcaggc tgccgaagcc cgtgtcgccg ccgctcgttc ggcctatctg
     1681 ccctccgcgg gtctcaccgc gtcgtatggc gggtcgggcg acctcagtga gttcgatctc
     1741 gccgatcgca ccagcctgac agtgggcgcc cagatttcgg ttcccctgtt taccggcggc
     1801 ttgaacgcct cgcgagtcgc ccaggcgctc gaaaccgcga acgccgccca gattgctgtc
     1861 gaaggcgaac gccgcggggt gctgcaggac gtcagctccg cctttgccca ggtcgtttcg
     1921 gcgcgcgcca ccgtcaccgc cggcaacgag gccgtgcgtg cggcgaccgt tgctgccgaa
     1981 ggtgtgcggc aggaagccca ggtcggcctg cgcaccaccc tggatgtact gaaccaggaa
     2041 ctggaactgc gctcggccca ggtgcttctg gtgaactcgg tcgccaacga atatatcgcc
     2101 caagcgtcgc ttctggcggc catgggacgt ctggaaggtc cagatctgga tccctctatc
     2161 gaggcctacg acgccgcagc caacacgaac gccgtgcgcg gtcgcggagc cttgccgtgg
     2221 gacggtgttc tggaggctat cgaccgcatc gccgccccac tggtgacccc ggcgacggac
     2281 gcgcctgatg cgccgattga tacccaactg aagtccgaca tcgtcaagac ggcgccccgc
     2341 tcctgaaggc tggcgcgagc ggttgccgac cttaaccgtg tagctgcaaa acgcccgttg
     2401 attccgtcgg gcgttgatgc gacgacatat gctggggggc agggcgagac cgtcgcctgc
     2461 cgccccccgc cgtccgtgca tgtcgcctga tgtgcgctcc caaggctcga tcgaggttta
     2521 cgaatgtccg aaccgaccgc tcaggaaccg acgatggaag agatcctagc gtcgattcgc
     2581 cggatcatct ccgaagacga cgcgccggtg gaaaccgtcg cggctccagc tcccgagccc
     2641 gaacctttcc cgagccccgt tgcggacgcc gctccgtccg cgcctgagcc tgtcgagccg
     2701 gaagaagcag aggacgacat cctcgaactg accgacacct atgaagcccc tgcagtcgaa
     2761 agcatcggag atctggacgt ttccacgccg gagcctgagg cgccagcgac gtggaatcct
     2821 tcgcccgaac cggaacccgc ccaatcctac gacagcctgg tcggcgaaag cgcagccgcc
     2881 agcgccgcgt ccgcttttgc gggactgcac gccagcttga agaagcccga gcccgctccg
     2941 gcggcttcca acgacctgaa cttcatcagc ggtatgaccg tcgagggtat ggtcgccgaa
     3001 atgctcaagc cgatgctgaa gaactggctc gacagcaacc tgccgggcat cgtcgaggcg
     3061 gcagtcaagg ccgaggtcga gcggatcgca cgcaccgccg gctagccctc ttcgcgtcac
     3121 gcgactagat caacaggggc ggctccgacg gggccgccct ttcgctttct aaaccgccct
     3181 catgccgcaa acggcgaggg cagaagaaga tcacgatgct cgacaagact ttcgatccca
     3241 aggcttccga accccgcctc tatgctcagt gggagcagtc cggcgccttc gccccgaccg
     3301 agggcgcggc cgaagcctat tcgatcgtga tcccgccacc gaacgtgacg ggctcgctgc
     3361 acatcgggca tgcgctgaac aacacgcttc aggacatcct gacccgctat caccggatga
     3421 agggcaaggc cgcgctgtgg ctgcccggca ccgaccacgc gggcatcgcg acccagatgg
     3481 tcgtcgagcg ccaactggcg gcggcgggca atatgggccg tcgggagatg ggccgtgacg
     3541 ccttcgtcga gaaggtctgg gagtggaagg ccgagagcgg cggttcgatc gtccagcaac
     3601 tgcgtcgtct gggcgcctcc tgcgactggt cgcgcgaacg cttcacgctc gacgaaggtt
     3661 tgaacgccgc cgtccgcaag gtcttcgtgc aactgcacaa ggaaggcctg atctatcgcg
     3721 acaagcggct ggtcaactgg gacccgcagt tccagacggc catctcggac ctcgaggtcg
     3781 agcagcgcga ggtcgaaggc ttctactggc atttcgccta tccgctggcg gacggcgtca
     3841 cctacgaaca ccccatcgcc ttcgatgaag agggcgtggc gacggaatac gaaacgcgcg
     3901 actacatcgt cgtcgcgacg acgcggcctg agaccatgct gggggacacc ggcgtagccg
     3961 tgcatcccgg cgatgagcgc tatgcgagcc tcgtcggcaa gttcgtgacg ctgccgatcg
     4021 tcggccgtcg cgtcccgatc gtcgctgatg actacgccga tccgaccaag ggctccggcg
     4081 cggtgaagat cacgcccgcg cacgacttca acgacttcca ggtcggcaag cgggcgggac
     4141 tggcgacgat taatatcctc gacgccttcg cccgcatcac cgatgccgac gcgcccgaca
     4201 ttccgacgga atacactggt caggatcgct tcgccgcgcg caaggcgatc gttgcgcgcg
     4261 ccgaggccga gggttggctc aaggagatcg agaagaccaa acacgtggtc ccgca
//
LOCUS       DASVTM010000351         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_726564,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000351 DASVTM010000000
VERSION     DASVTM010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726564"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF633_09520"
     CDS             complement(<1..435)
                     /locus_tag="VF633_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471339.1"
                     /translation="MTSQPLFRSLNISRVLDGVERIAVVALYILLVQRFAGDLAHKPG
                     NILLILSEGLIAIMVLCRRSTEDITLRPLDWLTGVLGTALPMLVHPTGGGSTGGAVLM
                     LAGLVISFGAKLSLRRSFGVIAANRGVKRTGLYAVVRHPMYLG"
     gene            complement(563..1153)
                     /locus_tag="VF633_09525"
     CDS             complement(563..1153)
                     /locus_tag="VF633_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471340.1"
                     /translation="MLLIALMIAGLSGQDTSSSAAGSPELYAAPVIRPYEPPSAFGRQ
                     IEEGDVARPIRSSPITGPVAVEAYRNNYEQTPSLGEVSYEQSVARARAVADARMGPLD
                     GVWKATLADGTSVLDMVLSDRGAVKPVEGALSLAGKVSAATAGVSVVRSEGEAVIEAS
                     VEGRPVRLSLQRTAEGWTGTLSGLGRDQTVVLAHPR"
     gene            1350..1799
                     /gene="sdhC"
                     /locus_tag="VF633_09530"
     CDS             1350..1799
                     /gene="sdhC"
                     /locus_tag="VF633_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX8471341.1"
                     /translation="MSSPNDSTTDDGRYVTQPNGRTRPLSPHLQVWRWHVTMTASILF
                     RITIGAISVGAAIMVGWLAAVASGAEAYAAVLEFMASPFGLLAGFGLTVVLFSFILNG
                     ARHLINDTGNGLTLRPATLLSNISVWGPIPLAVLFWVALFATGRIAL"
     gene            1796..2200
                     /gene="sdhD"
                     /locus_tag="VF633_09535"
     CDS             1796..2200
                     /gene="sdhD"
                     /locus_tag="VF633_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX8471342.1"
                     /translation="MSKPAMFRNGVKISERHGAGEWTIEKVCLAILAPLGVWVASSVF
                     TLTGADYDAVRAWSASPLNAGLAAVTALVFFGYVSIAWKVIIEDYIHKPGTKALLIGL
                     ANLIFLVLAAASVFFIVRLALGSAPLPAGIGA"
     gene            2205..>2781
                     /locus_tag="VF633_09540"
     CDS             2205..>2781
                     /locus_tag="VF633_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8471343.1"
                     /translation="MADLKFIDHEYDVVVVGAGGSGLRAALGAAQQGLKVACITKVFP
                     TRSHTVAAQGGISASLGNMGEDDWKWHMYDTVKGSDWLGDQDAIEYLVREAPKAVYEL
                     EHWGVPFSRTDEGKIYQRAFGGMTRNYGEGPVQRTCAAADRTGHAILHTLYGQSLNAA
                     VEFFTEYVALDLIMDEGECRGVTAWKLDDGSL"
BASE COUNT          499 a          942 c          852 g          488 t
ORIGIN      
        1 gccgagatac atcggatggc gcacgaccgc gtacaggccg gtccgtttca cgccgcggtt
       61 ggcggcgatc acgccgaagc tgcgacgcag cgaaagcttg gcgccaaagc tgatcacgag
      121 gcccgcaagc atcagcaccg ccccgccggt tgatccgcca cccgtcggat ggacgagcat
      181 cggaagcgct gtgccgagga ccccggtcag ccagtcgaga ggtcgcaacg taatgtcttc
      241 ggtcgagcga cggcacagga ccatgatcgc gatcaggccc tcggacagaa tcagcaggat
      301 gttgccgggc ttatgcgcaa gatccccggc gaaacgttgg acgagcagga tgtagagcgc
      361 aaccaccgcg atccgctcaa cgccgtccag aacgcgtgaa atattcagac tacggaacag
      421 gggttggctg gtcatggcca gtgaataccg ccgaccagat tgccgctgtc ttaagaagtg
      481 tttataaaac tgagggttat aactctagtg ggccgacaac tgctggatgc attcgccgtg
      541 aatcagcgat gtcttgtcgt ccttaacggg ggtgcgccag aaccaccgtc tggtcgcgac
      601 ccaagccgct gagtgtcccc gtccaaccct cggccgtccg ctgaaggctg aggcgcaccg
      661 gacggccctc gacagaggcc tcgatgactg cctctccctc tgatctgacc actgaaactc
      721 cggcagtggc ggcggagacc ttgccagcca ggctaagcgc gccctcgacg ggtttcaccg
      781 ctccacgatc cgacagaacc atatccagca ccgacgtccc atcggcgagg gtcgccttcc
      841 acaccccgtc cagcgggccc atccgggcat cggcgaccgc gcgggcgcga gcgaccgact
      901 gctcgtagct gacctccccc aacgacgggg tctgttcgta gttgttgcga taagcctcga
      961 cggccaccgg cccggtgatg gggctgctgc ggatcgggcg ggccacgtcc ccttcctcga
     1021 tctgacgacc gaacgccgac ggcggttcgt aggggcgtat aacgggagcg gcgtagagtt
     1081 ctggcgaacc ggcagcgctg gaggacgtgt cctggccgga aaggccggcg atcatcaggg
     1141 caatcagcag catggcctca cgatgaagat cgcgggtccg cctgactagt gcgaaccggc
     1201 aacacgcgaa aacgagatgc gttccgttcg ttcaaactat ttcttgccgc actgcaacca
     1261 cgcccgcgtt gacacccccg tgcgcggttc cctaaagcct ctgcacacgc aagaaagcct
     1321 gaattcacag ggttttgaga accattcgca tgtctagtcc gaacgactcg acgaccgacg
     1381 acggtcgcta cgtcacccag ccgaacggtc gcacccgacc gctgtccccc cacctgcagg
     1441 tctggcgctg gcacgtcacc atgaccgcct cgatcctgtt ccgcatcacc atcggcgcga
     1501 tcagcgttgg cgctgcgatc atggtcggct ggctggccgc cgtcgcctcg ggcgccgagg
     1561 cctacgctgc ggttctggag ttcatggcct cgccatttgg cctgctcgcc ggcttcggcc
     1621 tgacggttgt gctgttctcg tttattttga acggcgctcg ccacctcatc aatgataccg
     1681 gcaatggcct cacgctgagg cccgccaccc tgctgtccaa catctccgtc tggggcccga
     1741 tcccgctcgc ggtgctgttc tgggtcgccc tgttcgccac cgggaggatc gcgctgtgag
     1801 caagccagcc atgttcagga acggcgtcaa gatttccgag cgtcacggcg ccggcgagtg
     1861 gaccatcgaa aaggtctgtc tcgcgatcct cgcaccgctt ggcgtatggg tcgcctcgag
     1921 cgtcttcacc ctgaccggcg ccgactacga cgccgtacgc gcctggtccg ccagcccgct
     1981 gaacgccggc cttgcggcgg tgacggccct cgtcttcttc ggctacgtca gcatagcttg
     2041 gaaagtcatc atcgaagact acatccacaa gccggggacc aaagccctgc tgatcggcct
     2101 ggcgaacctg atcttcctcg tgctggccgc cgccagcgtc ttcttcatcg tgcggctggc
     2161 tctgggctct gccccgcttc ctgccggcat cggagcctga acgcgtggct gacttgaagt
     2221 tcatcgatca cgaatatgac gttgtcgtcg tcggcgccgg gggctcgggc cttcgtgcag
     2281 cgctcggcgc ggcgcagcag ggcctaaagg tcgcctgcat caccaaggtc ttcccgaccc
     2341 gctcgcacac cgtggctgcc cagggcggca tctccgcctc gcttggcaac atgggcgagg
     2401 acgactggaa atggcacatg tatgacaccg tcaaggggtc ggactggctg ggcgatcagg
     2461 acgccatcga atacctcgtc cgcgaggccc ccaaggccgt ctatgagctg gaacactggg
     2521 gcgtgccttt cagccgcacc gacgaaggca agatctacca gcgcgcattc ggcggcatga
     2581 cccgcaacta cggcgagggc ccggtgcaac gcacctgcgc cgccgccgac cgcaccggcc
     2641 acgccatcct gcacaccctc tacggccagt ccctgaacgc cgcggtggag ttcttcaccg
     2701 agtatgtcgc gctcgacctg atcatggacg agggcgagtg ccggggcgtc acggcctgga
     2761 agctggacga cggctcgctg c
//
LOCUS       DASVTM010000352         4369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_194447,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000352 DASVTM010000000
VERSION     DASVTM010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194447"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /gene="cyoA"
                     /locus_tag="VF633_09545"
     CDS             complement(<1..1074)
                     /gene="cyoA"
                     /locus_tag="VF633_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEX8471344.1"
                     /translation="MRRLLPWLALPALALLAGCNAVVLAPSGDVAAQQRDLLVISTLL
                     MLLIIVPVIGLIVFFAWKYRQSNKDAAYEPDWDHSTYLEVAIWGAPLLIIICLGALTW
                     AGTHLLDPYRPLDRIAAGRPASKTIKPLQVNVVSLDWKWLFIYPEYGIATVNELAAPV
                     DRPIQFTITSSSVMNSFYIPALAGQIYAMPGMQTTLYGVINEPGEYKGFSANYSGSGF
                     SGMRFKFHGLPQAGFDQWVAQVKAGGQSLDRDGYLRLEAPSENVPVMRFASVDSGLFS
                     AIVNLCVEPGKMCMHEMVAIDAKGGLDTESARNVMALTYDKDTRRGAVFGPAQTYVAT
                     LCTPTDPDPRDLRQTRLAAIASPA"
     gene            1250..2599
                     /locus_tag="VF633_09550"
     CDS             1250..2599
                     /locus_tag="VF633_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471345.1"
                     /translation="MTASTGASSTSVERDARKINTSGPHDKIDAGEIAIGVVIGRASE
                     FFDFFVFAIASVIVFPQLVFPFAGPLVGTLYSFALFALAFIARPIGTVIFIAIDKAFG
                     RGAKLTIALLLLGTSTVATAFLPGYETIGMWSVVLLALLRIGQGIALGGTWEGLASLL
                     AMNAPENKRGWYAMLGQIGAPIGLILASALFAFFVSLLSTADFLDWGWRYPFMVAFAI
                     NVVALFARLRISATPAFIESYEDRELQPTPVLQTVRAEGKNIVIGAFAPLASFALFHM
                     VTVFPLSWVLLYTQEPPGRFLIIELVGAFVCLGCVILSGVLADRYGRRNLLAATAAAI
                     AAFSGFAPQLLAGGEIGGIVFMISGFILLGLSFGQSSGAVASNFSPVNRYTASALTSD
                     LAWLFGAGFAPLAALLLASTFGLTSAGAYLLSGAAGTLIALWVNKEQAHHRKEAKAD"
     gene            2682..2960
                     /locus_tag="VF633_09555"
     CDS             2682..2960
                     /locus_tag="VF633_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471346.1"
                     /translation="MMYVVQVDDKSLRARSDPMPAMIALKVARAWQSHGDRGVQIIKA
                     DVDATGQRSWTVRDFIAFTREGSTRRARASQIRGYTTVVQAVQATTTP"
     gene            3524..>4369
                     /locus_tag="VF633_09560"
     CDS             3524..>4369
                     /locus_tag="VF633_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471347.1"
                     /translation="MTSASVLVLAAVAPAAQAQQTEVGAPGQALTAGTISTTTGTIND
                     NGANNGVVVITQSTPVVLVAGAAIDTSATANRTALDIGASQGATGPITVNFAGASNIV
                     ASGSGIQVSGVSQDTTLNTGSGSTIAATQTAVIGNTQAGFLTINNGANTISGDRGISA
                     TAFGPGTLGVVVNSTGGTISGVNYGINAVALGAAVTVGQGLGVTTVVTSTGASGTGIL
                     ANTTGTGAISVRSGAGGTINGGATGISALAAGGGAVTVNVGAAIGGTSAPTSRGVNAT
                     SFGGNV"
BASE COUNT          748 a         1411 c         1403 g          807 t
ORIGIN      
        1 ggcgggggag gcgatggcgg ccagacgggt ctggcgcagg tcgcgggggt cgggatctgt
       61 cggcgtgcag agcgtggcga cataggtctg ggccgggccg aagaccgcac cacgacgtgt
      121 gtccttgtca taggtcagtg ccatgacgtt gcgggcgctc tcggtatcga gaccgccctt
      181 ggcgtcgatc gccaccatct cgtgcatgca catcttgccc ggctccacgc acaggttgac
      241 gatggcgctg aacaggccgc tgtcgactga ggcgaagcgc atcaccggca cattttcgct
      301 aggggcctcg agccgcagat agccgtcacg gtccagcgac tggcctccgg ctttcacctg
      361 ggccacccac tggtcgaagc cggcctgggg caggccgtgg aatttgaacc gcatgcccga
      421 gaagcccgag ccgctgtagt tggccgagaa gcccttgtat tcgccgggct cgttgatcac
      481 gccatacagc gtcgtctgca tgcccggcat ggcgtagatc tgaccggcca gggcggggat
      541 gtagaacgag ttcatcacgg aggacgaggt gatggtgaac tggatcgggc gatcgaccgg
      601 agcggccagt tcattgaccg tggcgatgcc gtactccggg tagatgaaca gccatttcca
      661 gtcgagcgag accacgttga cctgcaacgg cttgatcgtc ttcgacgcag ggcgaccagc
      721 ggcgatccgg tcgagcggac gatagggatc cagcaggtgg gtcccggccc atgtcagggc
      781 tccgaggcag atgattatca agagcggtgc gccccagatg gcgacttcca gataggtcga
      841 gtgatcccag tcgggctcgt aggccgcgtc cttgttcgac tgacggtatt tccaggcgaa
      901 gaagacgatc aatccgatca ccggcacgat gatcaacagc atcagcaagg tcgagatgac
      961 cagcaggtcg cgctgctgag ccgccacgtc accggacggg gccagcacga cggcgttgca
     1021 ccccgccagc aaggcgagcg cgggcagcgc caaccatgga aggagacgcc tgatcacggg
     1081 attggtcggt agctggggag gcatagtgtg ggcctctagg ccggagagaa acggaacgac
     1141 attggacaat ctgccctatc tgtttttgac tcgtagagtt ccaataagcg cgccacaaca
     1201 ccgtgacccg tcgtcgcgcg catactcgat ctaaggaagc gatcacccga tgaccgccag
     1261 caccggagcg tcctccacgt ccgtcgaacg ggacgccagg aagatcaata cgagtggtcc
     1321 gcacgacaag atcgatgcgg gcgaaatcgc catcggcgtc gtgattggcc gcgcgtccga
     1381 gttcttcgac ttcttcgttt tcgcgatcgc ctcggtgatc gtgttcccgc aactggtgtt
     1441 cccgttcgcc ggcccgctgg tgggtacgct ctactccttc gctctgttcg ccctggcctt
     1501 catcgcccgc ccgatcggga cggtgatctt catcgccatc gacaaggcct tcgggcgtgg
     1561 ggccaagctg accatcgccc tgctgctgct gggcacctcg accgtggcga cggccttcct
     1621 gcccggttat gaaaccatcg gcatgtggtc ggtcgtcctg ctggccctgt tgcgcatcgg
     1681 tcagggcatc gcgctgggcg gcacctggga agggctggct tcgttgctgg ccatgaacgc
     1741 gcccgagaac aagcgcggct ggtacgccat gctgggccag atcggcgcgc ccattggcct
     1801 gatcctcgcc agcgccctgt tcgccttctt cgtctcgctg ctgtcgaccg ccgacttcct
     1861 cgactggggc tggcgctatc cgttcatggt cgcgttcgcc atcaacgtcg tggccctgtt
     1921 cgctcgcctg cgtatctcgg cgaccccggc ctttatcgag tcctatgagg accgcgaact
     1981 gcagccgacg ccggttcttc agaccgtcag ggccgagggc aagaacatcg tcatcggcgc
     2041 ctttgcgcca ctggccagct tcgccctgtt ccacatggtc acggtgttcc cgctatcgtg
     2101 ggtgctgctc tacacccagg agccgccggg gcggtttctc atcatcgagt tggtcggcgc
     2161 cttcgtctgc ctcggctgcg tgatcctgtc cggggtgctg gccgaccgat acggacgccg
     2221 caacctgctg gccgccacag ccgccgccat cgccgccttc tcaggcttcg cgccacagct
     2281 tctggcgggt ggcgagatcg gcggcatcgt cttcatgatc agcggtttca tcttgctcgg
     2341 cctgtcgttc ggccagtcgt cgggcgcggt cgcctcgaac ttctcgccgg tgaaccgcta
     2401 taccgcttcg gcgctgacgt cggatctggc gtggttgttc ggggcaggtt tcgctcccct
     2461 cgcagccctg ctgctggctt cgaccttcgg cctgacgtcg gcgggcgcct atctgctgtc
     2521 tggcgctgcg ggcacgctga tcgctctttg ggtaaacaag gaacaggccc accaccgcaa
     2581 ggaagccaaa gctgactgac cgcgcaacac ccccgtccta ggtctaaagt tacaggcgag
     2641 gccaacttcg cacgaccttg ggccgtgata gcttcgccgt catgatgtac gtcgttcagg
     2701 tcgatgacaa atcgctgcgg gcccgctctg accctatgcc tgcgatgatc gccctcaagg
     2761 tcgcccgggc ctggcagagc cacggggatc ggggcgttca gatcatcaag gccgatgtcg
     2821 acgccacggg ccagcgctcg tggaccgttc gggacttcat cgcgttcacc cgggagggca
     2881 gcacccgcag ggcgcgtgcc agccagatcc gcgggtatac gaccgtcgtc caggcggttc
     2941 aggcgacaac gacgccataa cgcacgctgg cgggttaatg gtccgttccg aaggggaagg
     3001 cgtacccggt attgatacag tgccgtgaga tgagccgccg tccagttctc atccgcttgc
     3061 gacgttcgag gctcgggttt ggtgggccag ccgccgaccg agaacgcctt cgcggtccgg
     3121 cgcaccgcct ccgcaccgcc tcggccaaca agacgtcagg ccgttcgaaa ccagcttccg
     3181 cgacatcggc ggaatgcgtt ggccctggat cccttacacc cgagcggccg gatcgccgac
     3241 cgctaactca ggtcctgaaa accaagggct gcgacttgga ggtctgcacg accgcgcctt
     3301 ttccccggcg gccgcagtac cggccttgag agcactcaat tcgccggtcc aagcgccaaa
     3361 atagacggtg attggttagt ccgtcccagc tttgggacgc tctgaacaat caatgctttg
     3421 tgcatgtttg ggcgcctaag agccgcacat gtaacgcggc cgagcatcgc cgcgcagggg
     3481 aagtccagtg attagatttg tagatcgccg ttcggctgcg ctgatgacca gcgcgtcggt
     3541 attggtcttg gccgctgtgg cgcccgccgc ccaagcccag cagacggagg ttggggcgcc
     3601 gggacaggcc ctgaccgccg gtacgatctc gaccacgacg ggcacaataa atgacaacgg
     3661 cgccaacaac ggcgtcgtgg ttatcaccca gtccaccccg gtggtcctgg tcgccggcgc
     3721 cgcgatagat acctccgcaa cagccaaccg cacggctctg gatattggag cgtcgcaggg
     3781 ggccaccggt ccgataaccg tcaatttcgc gggcgcctcg aacatcgtgg ctagcggctc
     3841 gggcatacag gtttctgggg tcagccagga cacgacgctc aataccggca gcggcagcac
     3901 gattgctgcc acccagacgg cggtcatcgg caacacgcag gccggcttcc tgacaatcaa
     3961 caacggcgcc aacacgatct cgggcgatag aggcatcagc gccaccgcat ttggccccgg
     4021 aaccctgggg gtggtagtga actccaccgg tggcacgatc tccggcgtca actacggtat
     4081 caacgccgtc gctctcggcg ctgcggtcac ggtcggtcaa gggctgggag tgacgacagt
     4141 tgtcacctca accggcgcca gcgggactgg tatcttggcg aacaccacgg gaacgggcgc
     4201 gatctctgtc cggtcgggcg ctggcggcac gatcaacggc ggcgcaacgg gcatcagcgc
     4261 tttggcagca ggcggcggcg ccgtcaccgt caatgtcggt gcggctatcg gtgggacttc
     4321 ggcccccacc tctcgtggcg tcaacgcgac ttcgttcgga ggcaatgtc
//
LOCUS       DASVTM010000353        15633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_351537,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000353 DASVTM010000000
VERSION     DASVTM010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 15633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15633
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351537"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VF633_09565"
     CDS             complement(<1..513)
                     /locus_tag="VF633_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX8471348.1"
                     /translation="MKYIGALLPVLVLLSGLAFAPVVAAQDTTPAPEAATALEDVVVT
                     ARRSGAPMWEVRRGDSTVLLVGEIRGVPAATPWRPDALEAATLRADRVFSGMRVQASF
                     SDLFRLIWRSRTFVSLPEGKTTADYLPPEWQVRLSALEVRYDQDYSRRSFLISSEDLL
                     TDRLRFDRATT"
     gene            661..2730
                     /locus_tag="VF633_09570"
     CDS             661..2730
                     /locus_tag="VF633_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471349.1"
                     /translation="MTKTILMATTALLFSAGAALAGTAPQVAEIPVPTGPTQTAPLAD
                     ATQRGVLVFTPDFFAEQRPNTALDMINRVPGFSIEDGSGSRGFEGSVGNVLINNARPA
                     SKNDTGSNVLSRTLASRVERIELIRGGAAGIDMQGYSVVVNVILKKGTSRQSILTYNG
                     ALFEGGHDGHGGSYQFTASNGDRTWGVTLGDGISTSDSNGVGRVVRRDANGAVIRDES
                     HSNDSYGGGNSIRGNYSGPLAGGKIDLTARLGLNDWHGIQTQTSAAVLRESRDDEDST
                     SGELGLVYTRPLNPRWTVEGRLIHQFEDFDNLSLSNTRLNGASDPEQAFTASGNSSES
                     ILRGLVRNERSAALTVEAGAEIAYNMLEVEQSFTVGGTNIPLPSASVKVEETRGEAFT
                     KATWRIHPDLTLEAGLRLEASTISQSGDAEQEKNLFFAKPRFLATWTPRPNNQIRVRF
                     ERELGQLDFGDFAASADLDDGTLYGGNVDLEPEQRWISELIYERRFWGEGIVSVGYRH
                     DRIIDVIDRLPLPGGLSATGNIGDGTLDQLSLNVVVPTDKLHIPGGRFTFRNDWNKTE
                     VTDPTTGEARPISNVRATQANIGFQQDIRGGKLQWGVNYLPLLGQKNFYPDQVSGWRG
                     SEYFELFTEYKPTPTLSIRAQLNLWDDFEIRRTVYSDRVAGTVAFTENRQIDPRTFWS
                     FKVRKTF"
     gene            complement(2947..4713)
                     /locus_tag="VF633_09575"
     CDS             complement(2947..4713)
                     /locus_tag="VF633_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX8471350.1"
                     /translation="MTDGGVAIEAPQKQFLGHPRGLVILFFTEMWERFSFYGMRALLV
                     LYLTQHFLFSSNNAQGIYAAYAALVYLMPVIGGAIADRFLGSRKAVTIGALLLVAGHF
                     LMAFEGSGGKEYIQYQGREFPIAVEGRDTDRQLFAVDGGMRTPILISPEGIKTVGPAV
                     PEPQGTVSATPVTQAGTVGFPAEIAAGGYAIRKAVDLGGQSVLFFALALIVVGVGFLK
                     PNISTVVGSLYEKDDPRRDSGFTIFYVGINLGSVLASALCAWLGIQYGWKYGFGLAGI
                     GMLAGLVTFWAGQKWLGNRADPPPSANLGKKTFGVPREAWIYILGILFVVPMWLIVQQ
                     HDAVEASLLYLVPAIFVGLLGYSAFFIKGDARWKMLAAIVLTMFSVVFWALFEQAGSS
                     LTLFADQSTAMPRGFNAAMTQTFNPGTIIIFGPILAALWLWLGKRGLEPNVPVKFGMA
                     LVGVGAGFLLLVWAAHTQANDAFKVPLIWLFLAYFLHSIAELMLSPVGLSMITKLSVP
                     RMVGMMMGAWFMASALAHIVAGLVAKSTSTETVGGVVTDPALALQTYAKVFQEIGIWG
                     VGIGVLLLLGSPILKWMMKGVR"
     gene            complement(4855..8253)
                     /locus_tag="VF633_09580"
     CDS             complement(4855..8253)
                     /locus_tag="VF633_09580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX8471351.1"
                     /translation="MSASSNWSGEYAELGVMTNFSFLEGASHPGEQMIQAKALGLSAL
                     GVADRNTLAGMVRALMGSEDFDVPLVVGCRLGFLDGTELIVFPRDRAAYGRLCRLLTL
                     GKSEILSDPTAEPVEPARSNIVDFPGGGARSTDEVSPEIDPDPADRTTKGETRLTFEQ
                     ALAFAEGMIALAVAPIIPDVAFEARLKDWRERWPDTLYLAAQPLWRGGDRARLNRLAA
                     MAERTGAPLIATNAVLYHHPERRPLQDVLTCIREGTTIDAAGVRLQANAERHLRAPAA
                     MARLFRGHEAALHRTMDVVRACQFSLRELSYDYPDEPVPDGCSPQGWLIRLTCKGARA
                     KWPQGVPTKVVETIRKELRLVRELKYAPYFLTVHDVVAWARDPERDILCQGRGSAANS
                     VICFCLGITNVDPTRQEMLIERFISSERSEPPDIDVDFEHERRELVMQYVFDRYGRDR
                     AAIVATIIHYRPRSAIRDVGKVLGLTEDVTARMADTVWGSFSDAVKNDHVDRTGLNRD
                     DARMALALQLTSELIKFPRHLSQHVGGFVLSKGPLCEIVPIGNAVMKDRTFIEWDKDD
                     IDHLRIMKVDVLALGMLTAMKRAFAMIGESYGKPLELHTVPLESPEVYEMLCKADSLG
                     VFQVESRAQMSMLPRLQPREFYDLVVQVAIVRPGPIQGGMVHPYLKRRTERRAVEKLG
                     QVYDFEMPGPVGGDPDELRNVLRKTLGVPLFQEQAMKIAMVAAEFTGNEANGLRRAMA
                     TFRHMGTIGDYERIFVGRMSERGYDPEFAQRCFDQIKGFGEYGFPESHACSFAFLVYI
                     SAWVKCFYPDAFAACLLNSQPMGFYAPAQIVRDAREHGVEVRHPDVNASGWDASLEPD
                     ATLDRRALRLGMHSIDGFRAVWAAQIVEARDAAPFVDLEDLRLRAKLPPPALDKLAEA
                     DAFGSLKLSRRQGLWAAKGAPPASSAPLFEAMGLNEADGQPPDALPKLTPGEEVVGDY
                     QTIRMSLKGHPVAFLRQRLAQAGALTALDYQAAPAGRRVAVGGVVLVRQRPGSAKGVV
                     FLTLEDETGIANLVVWPDVFERLRPIAMGARMVLAKGRLQRAEGVSHLVVEDLIDWTP
                     MLAHISDDSLVLDTSPGSGHSPARPGRGQHPRDVRILPGSRDFH"
     gene            complement(8250..9866)
                     /locus_tag="VF633_09585"
     CDS             complement(8250..9866)
                     /locus_tag="VF633_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX8471352.1"
                     /translation="MMRRIVSVWLIDWPVTVWANGAGRVSPPELPVGAHEGPPFALIT
                     RTARGAVIHALNAAARAAGLSRGQTQADARAILPTLICRPADLAADQAALERLAVWAE
                     RWSPTVTIDQTQGALEGLFLDVTGAGHLFGGEAALLERIEARLAEAGARARTALAETP
                     GAAWALARWGAERPVVPEGRMKAALEGLPIEALRLDDSTLKQARRFGLKRIGDLYDMP
                     RAGLARRFRDGDGIGLVKRLDQALGLAAEALIPTRPPPKYRAWEAHFEPLCDTAGVEG
                     RLPELAAELAAALEKDGQGARVLTLTGFRTDGQTTSLTIKSGLPGRDVVVWMRLFRER
                     GIERLDLGFGVDALMLSADLTEPVRPHQVSIEDEAAARHAESLTALIDRLSARLGEGA
                     VRVAEPQGSWLPERTERWRPAIAGSPKRLRMDAAAADADPRQRPILLLDPPEPVEAIA
                     ELPDGAPALFTWRKLARRVSRAEGPERLSPEWWRPRADDRETRTRDYYRIEDDQGLRY
                     WLFREGLYGREYTGGDDQRSPSWWMHGMFP"
     gene            complement(9781..10356)
                     /locus_tag="VF633_09590"
     CDS             complement(9781..10356)
                     /locus_tag="VF633_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471353.1"
                     /translation="MAGAFVFALSRLPVEDERPVILAANRLWAGENGRPHGPGFRASG
                     LVGRGAGLVLADGRTEGELLWIMEQALRSGAVSAALATVEAASLAQTRRLEFAARDGA
                     AVGIVLRRTEGGLSAARRRWRISTLASRADPYDAKAPGGFRLRAELTRSRSERPGAWK
                     LEQDDETYRLRLADRLAGDGLGERGRTGLAA"
     gene            10647..11813
                     /locus_tag="VF633_09595"
     CDS             10647..11813
                     /locus_tag="VF633_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX8471354.1"
                     /translation="MSLRPFEADDYAAVNALHKDVWWPERSAAGWDWLASNPARLDIG
                     APSGWVIEGKDDWGDDVRAAAFMGNMVQRVWKDDALHHVATGFSVIVPPTMRGKSRQL
                     IRAVLDQPNISAGYTLNANARSAPLYSRHGMAAWPARTNDLKLSWVIDPIDCLRGRVL
                     REALKRAPGLIDPYRERFMRSGRPSLAARALGRARPVRYPSQVSALNDLSDSSAYDDF
                     WQALKSEGRVVADRSPEILRWRTSDPDRTEPPLMLVYMRDGAVTGFAMAVLAKTTPIE
                     PVFLEILDLVALQDEPHAIPALMRALVDLARARGAAKVRLQVVNEELKRRLGPWALAA
                     RREGGWGHGHIRFRPGGESLQTWSPTPFDGDHAFCQRPAPARGTPITGNRRPAV"
     gene            complement(11814..12242)
                     /gene="mscL"
                     /locus_tag="VF633_09600"
     CDS             complement(11814..12242)
                     /gene="mscL"
                     /locus_tag="VF633_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX8471355.1"
                     /translation="MSLVSEFREFAIKGNVVDLAVGVIIGAAFNSIVKSLVDQIVMPP
                     IGLLTGGIDFSKLEWVLRAENPATEAIEKVSIQYGAFLNTVIQFAIVAFVVFMVVKAI
                     NKLRRQEAAHPEVAAPPPAPTTTEVLLAEIRDELKARPSV"
     gene            complement(12265..12555)
                     /locus_tag="VF633_09605"
     CDS             complement(12265..12555)
                     /locus_tag="VF633_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX8471356.1"
                     /translation="MIKSELIEKLAAENTHLTHAEVERVVNVVLGRMTEAMADGGRVE
                     LRGFGAFSVRSRPARAGRNPRTGETVDVPAKSVPFFKSGKELRERLNASGDA"
     gene            complement(12792..14495)
                     /gene="rpsA"
                     /locus_tag="VF633_09610"
     CDS             complement(12792..14495)
                     /gene="rpsA"
                     /locus_tag="VF633_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX8471357.1"
                     /translation="MSDTHSPSRDDFAALLDEQLQGRDLGEGQVVHGRVVGIEKDIII
                     IDVGLKTEGRIPAREFGIGEGSVIPKVGDDVEVYLERVENALGEAVISRDKARREEAW
                     TRLEVVFAEGVPVNGAIVGRVKGGFTVDLGGASAFLPGSQVDIRPVRDVGPLMGKEQP
                     FQILKMDRPRGNIVVSRRAILEEARAEQRTELVGQLQEGEVRDGVVKNITDYGAFVDL
                     GGIDGLLHVTDMSWKRVSHPSQVLAVGDTVKVQIVKINPDTQRISLGMKQLQSDPWDG
                     VEAKYPVGAKYTGRITNITDYGAFVELEAGVEGLVHVSEMSWTKKNVHPGKIVSTSQE
                     VDVVVLDVDASKRRISLGLKQAQDNPWDAFVASHPIGSTVEGEVKNATEFGLFIGLDN
                     DIDGMVHLSDLDWSVSGEEAIQRYRKGEMVKAKVLDVDVEKERVSLGIKQLGGDPVGE
                     GDLYKKGQTVTVTVTSIETGGIEVKFGDDDAPVTSFVRKSDLSRDRNEQRPERYAVGD
                     RVDAQITSIDKATRRIAVSIKALEMADEKDAIEQFGSSDSGASLGDILGAALREKAKQ
                     D"
     gene            complement(14662..15312)
                     /gene="cmk"
                     /locus_tag="VF633_09615"
     CDS             complement(14662..15312)
                     /gene="cmk"
                     /locus_tag="VF633_09615"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX8471358.1"
                     /translation="MTAPLVIAVDGPAASGKGTVAARLATTFGLPFLDTGLLYRAVGL
                     KVLDAGGSLDDEAAAEIAARTLDANGLSDDPRLTTGEAGEAASRVAGHPGVRAALLDL
                     QQAFASQPGGAVLDGRDIGTVIAPNAPAKLFITATPETRATRRWKQLTARGDDISFED
                     MLADIVRRDERDAGRGSAPMIQAQDAVLLDTTEMDIETAFDAARRIVEAARAKHGL"
     gene            complement(15309..>15633)
                     /locus_tag="VF633_09620"
     CDS             complement(15309..>15633)
                     /locus_tag="VF633_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX8471359.1"
                     /translation="HGVTVMRGVGEMRVKESDRIRLMVDGLRACGVVVEEEPDGFLVT
                     GGSVPGGATVHTAHDHRIAMSHLVLGLAAAAPVTVDEPDMIATSFPGFAEMMVGLGGH
                     VASAE"
BASE COUNT         2781 a         5140 c         5092 g         2620 t
ORIGIN      
        1 ggtcgtagcg cgatcgaacc gcagccgatc agtcagtagg tcctcgctcg atatcaggaa
       61 gctccgccgg ctgtagtctt ggtcatatcg aacctcaagg gcggacagtc ggacctgcca
      121 ttcgggcggc agataatcgg ctgtcgtttt gccttcaggc agacttacga aggtccggct
      181 gcgccagatc agacgaaaca ggtcgctgaa ggaggcctga acccgcatcc cggagaacac
      241 ccgatcagca cgcagggttg cagcctcgag cgcatcggga cgccaaggcg tcgccgcagg
      301 cacgccccgg atttctccga ccagcagcac ggtgctgtcg ccccgcctga cttcccacat
      361 cggcgcgcca gaacgtcggg cggtgacgac gacgtcttcc agagcagtcg cagcctcggg
      421 cgccggcgtc gtatcctgag ccgccaccac gggtgcaaac gccagtcctg acaagagaac
      481 cagaacgggg agcagagcgc cgatgtactt catgattgaa tactgaaccc gcaattatag
      541 aatatccaca taaccttcct gtaatgatgt cggcgattta atccaccttt cagtcttgta
      601 actggagacg gagcgcctct gaggccagaa caccctcaca aacgagggga accccaggcg
      661 atgaccaaga cgatccttat ggccaccacg gcccttttat tcagcgcggg cgccgcgctc
      721 gcaggcacgg ccccacaggt cgccgagatc cctgtaccga ccggtccgac gcagactgcg
      781 ccgcttgccg acgccaccca gcgcggagtt ctggtgttca cgcctgactt cttcgccgaa
      841 cagcgcccga atactgcgct ggacatgatc aaccgggtgc ccggcttctc catcgaggac
      901 ggctcgggca gccggggctt cgagggctcg gtcggcaacg tgctcatcaa caacgcccgt
      961 ccggcatcca agaacgacac gggttccaac gtcctgtcgc gcaccctcgc cagccgggtc
     1021 gagcggatcg aactgatccg gggcggcgcg gccggcatcg acatgcaggg ctattcggtg
     1081 gtcgtgaacg tgatcctgaa gaagggcacc agccgccagt cgatcctgac ctacaacggg
     1141 gccctgttcg aaggcggtca tgacggccat ggcggaagct accagttcac cgcttccaac
     1201 ggcgaccgca cctggggcgt gaccctgggc gacggcatca gcaccagcga ttccaacggt
     1261 gtcggtcggg tcgtccgccg cgatgcgaac ggcgccgtca tccgcgacga aagtcattcc
     1321 aatgacagct acggcggcgg caactcgatc cgcggcaact attccggtcc gctcgcaggc
     1381 ggcaagatcg atcttaccgc gcgtcttggg ctcaacgact ggcatggaat tcagacccag
     1441 acctcggcgg ccgtcctgcg cgaaagccgc gacgacgaag acagcaccag cggcgaactg
     1501 ggcctcgtct acactcgccc tctgaatccc cgctggacgg tcgaaggccg gctgattcat
     1561 cagttcgagg atttcgataa tctgtcgctc agcaacacga gactgaacgg ggcgtcggac
     1621 ccagagcagg ccttcaccgc cagcggaaac tcgtccgaaa gcatcctgcg cggccttgtt
     1681 cgcaacgaac gctccgccgc cctgaccgtc gaggcgggcg cggaaatcgc ctacaacatg
     1741 ctcgaggtcg aacagtcctt caccgtcggc gggaccaata tccccctgcc ctcggcgtcg
     1801 gtgaaggtcg aggaaacccg cggcgaggcc ttcaccaagg ccacctggcg catccatccc
     1861 gacctgacgc tggaagccgg cctgcgtctg gaggcctcga ccatcagcca gtccggtgac
     1921 gccgagcagg agaagaacct tttcttcgcc aagccgcggt tcctcgccac ctggacgcca
     1981 cggcccaaca accagatccg cgtccgcttc gagcgcgagc tgggtcagct ggatttcggc
     2041 gacttcgcgg cctcggccga cctggacgac ggcaccctct acggcggcaa cgtcgacctc
     2101 gaacccgaac agcgctggat ctcggagctg atctatgaac gccgcttctg gggcgagggc
     2161 atcgtctcgg ttggttatcg ccacgaccgg atcatcgacg tgatcgaccg cctgcccctg
     2221 cccggcggcc tgtcggcgac cggcaacatc ggcgacggaa cgctggacca gctgtcactg
     2281 aacgtggtcg ttccgacaga caagctgcac atcccgggcg gccgcttcac cttccgcaac
     2341 gactggaaca agaccgaggt caccgacccc accaccggcg aggcgcgccc gatctcgaac
     2401 gtccgggcga cccaggccaa tatcggcttc cagcaggata tccgtggcgg caagcttcag
     2461 tggggcgtca attatctgcc gctgctcggg cagaaaaact tttatcccga tcaggtctcg
     2521 ggctggcgcg gctctgaata tttcgagctg ttcaccgaat acaagccgac gccgaccctc
     2581 tcgatccggg cccagctgaa cctgtgggac gatttcgaga tccgcaggac cgtctactcc
     2641 gatcgtgtcg ccgggaccgt cgccttcacc gagaaccgcc agatcgaccc ccgcaccttc
     2701 tggtccttca aggtccggaa gacattctga gaagcgggtc gcttcgcgac gcgctaacgc
     2761 tcgcgacgcg ctaacgctcg cgacgcggtt gctcgctgct tgagcgcgag ggaggtttgg
     2821 ggcagtcgaa cgcgaacgcg tgggctcaag cagtgaggct ccgcgagccg aacgtagccc
     2881 cccaaaacaa gaaagggcgg tgtccgcgtg gacaccgccc tttccattat ccgatgaggc
     2941 ggagcgctac cgtacgccct tcatcatcca tttcaggatg ggcgaaccca gcaacagcag
     3001 gacgccgatg ccgacgcccc agatgccgat ttcctggaag accttcgcgt aggtctggag
     3061 agccagcgcc gggtcggtga cgacgccgcc gacagtctcg gtcgaggtcg atttggcgac
     3121 cagaccggcg acaatatgcg ccagagccga ggccatgaac catgcgccca tcatcatgcc
     3181 aaccatccgg ggcaccgaga gcttggtgat catcgacagg ccaaccggcg acaacatcaa
     3241 ctcggcgatg gagtgcagga agtaggccag gaacaaccag atcagcggca ccttgaaggc
     3301 gtcgttagcc tgggtatgcg ccgcccagac gagcagcagg aagcctgccc cgacgccaac
     3361 gagcgccatc ccgaacttga ccgggacgtt gggctcgagc ccccgcttgc cgagccacag
     3421 ccacagggcc gccagaatcg gtccgaagat gatgatcgtg ccggggttga aggtctgcgt
     3481 catggcggcg ttgaagccgc gtggcatggc cgtcgactgg tccgcaaaca gcgtcaggct
     3541 ggagccggcc tgttcgaaca gggcccagaa gaccaccgag aacatggtca gcacgatcgc
     3601 cgccagcatt ttccagcgtg cgtcgccctt gatgaagaac gccgaatatc ccagcaggcc
     3661 gacgaagatc gccgggacca gatagagcag ggaagcctcg accgcgtcgt gctgttgcac
     3721 gatcagccac atgggcacaa caaacaggat gccaaggatg tagatccagg cctcgcgcgg
     3781 aacgccgaac gtcttcttgc cgagattggc cgatggcgga ggatcagcgc gattgccgag
     3841 ccatttctga ccggcccaga aggtcaccag acccgccagc atgcctatgc ccgccaagcc
     3901 gaagccgtat ttccagccgt actggatacc aagccaggcg cacagcgcgg aggccagcac
     3961 cgaaccgaga ttgatgccga cgtagaagat ggtgaagccc gagtcgcgac gcggatcgtc
     4021 cttctcgtag agcgaaccca cgaccgtcga gatgttgggc ttgaggaagc cgacgccgac
     4081 gacgatcagg gcgagagcga agaacagcac cgattggccg cccagatcga cagccttgcg
     4141 gatggcgtag ccgcctgcag cgatctcggc ggggaatccg acggttccgg cttgggttac
     4201 gggagtcgcg gaaacggtgc cctgaggttc gggcacagcc ggccccaccg tcttgatgcc
     4261 ctcggggctg atcaggatcg gcgttcgcat cccgccgtcg accgcaaaca actgcctgtc
     4321 cgtgtcgcga ccttcgaccg cgatagggaa ctcgcgcccc tgatactgga tgtattcctt
     4381 gccgcccgat ccctcgaacg ccatcaggaa gtgacccgcg accagcagga gcgcgccgat
     4441 ggtgacggcc ttgcgcgagc ccaggaagcg gtcggcgatg gcgccgccga tgaccggcat
     4501 caggtacacc agcgcagcgt aggcggcgta gatgccctgg gcgttgttcg agctgaacag
     4561 gaagtgctgg gtcagataca ggaccaacag ggcccgcatc ccgtagaacg aaaaccgctc
     4621 ccacatctcc gtgaagaaca ggatcaccaa cccccgtggg tgtcctagaa actgcttctg
     4681 aggcgcctca atggccactc ccccgtccgt catactcaag cgactgctcc ttcaggtgcg
     4741 ccgatccggc gcgtgttggg gcctaagcag cacgacgcga aagaccggac aagtcttaag
     4801 gatgaatcca aagcgtcgtc gcagtgtcgc atgcgtggat ttcaccgtcg gatttcagtg
     4861 aaagtccctc gacccgggca ggattcggac atcccgaggg tgctggccac ggccggggcg
     4921 ggcgggggaa tggcccgatc cgggcgacgt atcgaggacc agcgagtcat cgctgatgtg
     4981 ggccagcatg ggggtccagt cgatcaggtc ctcgaccacg aggtggctga cgccctccgc
     5041 gcgctggagc cgacccttgg ccagcaccat ccgcgcgccc atggcgatgg gccgcaaacg
     5101 ctcgaacacg tcgggccaga cgaccagatt ggcgatcccg gtctcatcct cgagcgtcag
     5161 gaacacgacg cccttggccg agccgggcct ttggcgcacc agcaccacac caccaacggc
     5221 gacgcgacgc ccggccggag ccgcctgata atccagcgcc gtcagggccc cggcctgcgc
     5281 cagccgttgg cgcaggaagg cgacggggtg gcctttcaag gacatgcgga tggtctggta
     5341 atcgccgacg acttcctcgc ccggcgtcaa tttgggcagg gcgtcgggcg gttggccgtc
     5401 ggcctcgttc agccccatgg cctcgaacag gggcgcgctg gaggccggcg gcgcgccctt
     5461 ggctgcccac agtccctgcc tgcgtgacag tttgagcgat ccgaaagcat cggcctcggc
     5521 cagcttgtcc agcgccggtg gcggcagctt tgcccgcaaa cgcagatcct cgaggtcgac
     5581 gaagggagcg gcgtcacggg cctccacgat ctgcgccgcc cacaccgccc tgaacccgtc
     5641 gatggagtgc atgccgagac gtaaggcccg gcggtcgagg gtcgcatccg gctccagcga
     5701 ggcgtcccat ccgctggcgt tgacgtcggg gtgccgcacc tcgacgccgt gctcgcgcgc
     5761 atccctcacg atctgggcgg gggcatagaa acccatcggc tggctgttca acagacaggc
     5821 cgcgaaggcg tcggggtaga agcatttcac ccaggccgag atgtagacga ggaaggcgaa
     5881 cgagcaggcg tggctttcgg gaaagccgta ttcgccgaag cccttgatct ggtcgaaaca
     5941 gcgctgggcg aactcgggat cgtagcctcg ctcgctcatg cgaccgacga agatgcgttc
     6001 gtagtcgccg atcgttccca tatgtcggaa ggtggccatg gcccggcgca ggccgttggc
     6061 ctcgttgccg gtgaactcgg cggcgaccat ggcgatcttc atcgcctgtt cctgaaacag
     6121 gggcacgccg agcgtcttgc gcagcacgtt cctgagctcg tccgggtcgc caccgaccgg
     6181 gccgggcatc tcgaaatcat agacctgacc cagcttctcc accgcccggc gttcggtgcg
     6241 gcgtttcaga tagggatgga ccatcccgcc ctgaatcggc cccggtcgca cgatcgccac
     6301 ctgaaccacc agatcataga actccctcgg ctgcaggcgc ggcagcatgg acatctgggc
     6361 ccggctctcg acctgaaaca cgcccagaga gtcggctttg cacagcatct catagacctc
     6421 ggggctttcg aggggaacgg tgtgcaactc cagcggcttg ccatagctct cgccgatcat
     6481 tgcgaacgcc cgcttcatcg ccgtcagcat gcccagcgcc agcacgtcga ccttcatgat
     6541 cctcagatga tcgatgtcat ccttgtccca ttcgatgaag gtgcggtcct tcatcaccgc
     6601 attgccgatg ggcacgatct cgcacaacgg ccccttgctg aggacgaagc cgccgacgtg
     6661 ctgggacagg tggcggggga atttgatcag ctcagatgtc agttgcaggg ccagcgccat
     6721 gcgggcgtcg tcgcggttca ggccggtgcg atcgacgtgg tcgttcttca ccgcatcgct
     6781 gaacgaaccc cagacggtgt cggccatccg ggcggtgacg tcctcggtca ggcccagcac
     6841 cttgcccacg tcgcggatcg ccgagcgcgg gcgatagtgg atgatggtcg ccacgatcgc
     6901 ggcgcggtcg cggccatatc ggtcgaagac atactgcatc accagttcgc gccgctcgtg
     6961 ctcgaagtcg acatcgatgt cgggcggctc cgagcgttcg ctggagatga agcgctcgat
     7021 cagcatctcc tgacgggtcg gatcgacgtt ggtgatcccg agacagaagc agatgactga
     7081 gttggccgcc gagccccgcc cctgacacag gatgtcccgc tccggatcgc gggcccaggc
     7141 gaccacgtcg tggaccgtca ggaaatacgg ggcgtatttg agctcacgca ccagccgcag
     7201 ctccttgcgg atggtctcaa cgaccttcgt aggtacgcct tgcggccatt tcgcccgagc
     7261 gcctttgcag gtcaggcgga tcagccagcc ttggggcgag cagccgtccg gcaccggctc
     7321 gtccgggtag tcatagctga gttcacgcag ggagaactgg caggcgcgca ccacgtccat
     7381 ggtgcggtga agcgcggctt cgtggccccg gaacaggcgc gccatggcgg cgggtgcgcg
     7441 caggtgccgc tcggcattgg cctgaagccg gacccccgca gcgtcgatgg tcgtgccttc
     7501 gcgaatacag gtcaggacgt cctgcagcgg acggcgttcg gggtggtgat aaaggacggc
     7561 gttggtggcg atcagtggcg cgccggtgcg ctcggccatg gccgccagcc ggttcagacg
     7621 cgcgcggtcg ccaccgcgcc acaggggttg ggcggccaga tagagggtgt cgggccagcg
     7681 ctcgcgccag tccttcagtc gggcctcgaa ggcgacatcg ggaatgatag gcgcgacggc
     7741 gagcgcgatc atgccctcgg cgaaggccag cgcctgttcg aaggtcaggc gggtctcgcc
     7801 cttggtggtg cggtcggcgg gatcggggtc gatctccggc gagacctcat cggtcgaacg
     7861 cgcgcctccg ccgggaaagt cgacgatgtt ggaccgggcg ggctcgaccg gctccgccgt
     7921 tggatcggac agtatctcgc tcttgccgag ggtcagcagg cggcacaggc gaccataggc
     7981 agctcggtcg cgggggaaga cgatcagttc ggtcccatcg aggaagccca gccgacagcc
     8041 gaccaccagc ggcacgtcga aatcttccga tcccatcagg gctcgcacca tcccggccaa
     8101 ggtgttgcgg tcggccacac cgagggcgga caggcccagc gccttggcct ggatcatctg
     8161 ttcgccgggg tgggaggctc cctcgaggaa gctgaaattg gtcatgacgc cgagctcggc
     8221 gtattcgccc gaccagttgg aagacgcgct cacgggaaca ttccgtgcat ccaccatgac
     8281 ggtgaacgtt ggtcgtctcc gccggtgtat tctcgcccgt acagcccctc gcggaacagc
     8341 cagtagcgca ggccctgatc gtcctcgatg cggtagtaat cgcgcgtgcg ggtctcgcgg
     8401 tcgtctgcgc gcggacgcca ccattcgggc gacagccgtt caggcccctc ggcgcgcgac
     8461 acccgtcggg ccagcttgcg ccaggtgaac agggcgggcg caccgtcggg cagctcggcg
     8521 atggcctcga ccggttcggg tggatcgagc agcaggatcg gacgctgacg tggatccgcg
     8581 tcggcagccg ctgcatccat tcggagccgc ttgggcgatc ctgcgattgc cggtcgccag
     8641 cgttcggtgc gctcgggcag ccacgaccct tgcggttcgg cgacgcgcac cgcgccctcg
     8701 cccagccggg ccgagagacg gtcgatcagg gcggtcaggc tttcggcatg gcgggcggcg
     8761 gcctcgtcct cgatcgagac ctgatgcggc cggaccggtt cggtcagatc agctgacagc
     8821 atcagggcat cgacgccgaa gccgagatcg agacgctcta tgcctcgttc ccggaacagc
     8881 cgcatccaga ccaccacgtc gcgacccggc aggcccgact tgatcgtcag ggaggtggtc
     8941 tgaccgtccg tgcggaagcc ggtcagggtc aggacgcgcg caccctgacc gtccttttcg
     9001 agcgcggcgg cgagttcggc ggcgagttca ggcagccgcc cctcgacccc cgccgtgtcg
     9061 cacaggggct cgaaatgcgc ctcccacgcc cgatatttcg gcggcgggcg ggtcgggatc
     9121 agagcctcgg cggcaaggcc cagagcctga tccagccgct tgaccaaccc aatgccgtcg
     9181 ccatcgcgga agcggcgggc cagacccgcg cggggcatgt cgtagaggtc gccgatccgc
     9241 ttcagcccga aacgccgcgc ctgtttcagg gtcgagtcgt cgagccgcag ggcctcgatc
     9301 ggcaggcctt caagcgcagc cttcatgcgg ccttcgggaa ccacagggcg ttcggctccc
     9361 caacgcgcca gagcccatgc cgcgccaggc gtctcggcca gagccgttcg agctcgcgcc
     9421 cccgcctcgg ccagcctcgc ctcgatccgt tccagaagcg ccgcctcgcc gccgaacaga
     9481 tggccggcgc cggtgacgtc cagaaacagt ccctcgagcg cgccttgcgt ctggtcgatc
     9541 gtgacggtcg gcgaccagcg ctcggcccag acggccagac gttcgagcgc tgcctggtcg
     9601 gcggcgagat cggcaggacg acagatcagc gtcggcagga tggcgcgggc gtcggcctgg
     9661 gtctgaccac gcgacaggcc agccgcccgc gccgccgcgt tcagggcgtg gatgaccgcg
     9721 ccccgcgctg tgcgggtgat cagggcgaag ggcggacctt catgagcgcc aaccggaagc
     9781 tcaggcggcg agacccgtcc ggccccgttc gcccagaccg tcaccggcca gtcgatcagc
     9841 cagacggaga cgatacgtct catcatcctg ctccagtttc cacgcgcccg gtcgctcgct
     9901 gcggctgcgg gtcagttcgg cgcgcaggcg gaaaccaccc ggcgccttgg cgtcgtaggg
     9961 atcggcccga ctggccagtg tcgagatgcg ccagcgacgt cgcgcggcgc tgaggccacc
    10021 ctcggtccga cgcaggacga taccgaccgc agccccgtcg cgggcggcga attccagccg
    10081 gcgggtctgt gccagagacg ccgcctcgac cgtggccaga gccgccgaga ccgccccgga
    10141 tcgcagcgcc tgttccatga tccacagcag ttccccttcg gtccgaccgt cggccagaac
    10201 cagaccggcg cctcggccaa ccagccctga cgcccgaaat cccggaccat gaggtcttcc
    10261 gttttcgcct gcccacaggc gattggcggc gaggatcacc gggcgctcgt cctcgaccgg
    10321 cagacggctt aaggcgaaga cgaaggcccc ggccatgtct ctcgaactgt ccgcccgcac
    10381 ctcctccagg ccctcggcgc gaactggcca gacggtagaa cccgggaggg ttcgagcgag
    10441 catgtgcggg tcggggggaa cggacatcgg gtggcttgaa cctcgatgtt cattatttgt
    10501 tctcatgcct gcggagtcaa atcctgaatc ggtttgcgcg cgcgcgaact gtcgatccac
    10561 ctgtggttgc ctcgcctatt ggcccgcagc gtgcagcgtt tgaatgccta caccccagcc
    10621 gtcccgcact gggagagaga cgttcgatga gcctgcgccc gttcgaagcc gacgattatg
    10681 cggccgtcaa cgccctgcat aaggacgtct ggtggcccga gcgttcggcc gccggatggg
    10741 actggctggc gtcaaatcca gcacggttgg acattggcgc tccgtctgga tgggtgatcg
    10801 agggcaagga cgattggggc gacgacgtgc gcgccgccgc cttcatgggc aatatggtcc
    10861 agcgggtgtg gaaggatgac gcactgcatc acgtcgccac cggcttttcg gtcatcgtgc
    10921 cgccgacgat gcgtggcaag agtcggcaat tgatccgtgc cgtgctcgac cagccgaaca
    10981 tcagcgccgg ctatacgctg aatgccaacg cccgttcggc tccgctttac tcacgccatg
    11041 gcatggccgc ttggccggcc cggacgaatg atctgaagct gtcgtgggtg atcgatccta
    11101 tcgactgcct gcgcggccgg gttctacgcg aagcgttgaa gcgggcaccc ggcctgatcg
    11161 atccctatcg cgaacgcttc atgcggtccg ggcgtcccag cctcgcggcc cgcgctctcg
    11221 gacgggcccg accggtgcgg tatccgtcgc aggtttcggc gctgaacgat ctgagcgaca
    11281 gctccgccta tgacgatttc tggcaggcgc taaagagcga aggccgggtc gttgccgatc
    11341 gcagccctga aatcctgcgc tggcgcacca gtgatccgga ccggaccgag ccgccgctga
    11401 tgctggtcta tatgcgagac ggcgccgtca ccggtttcgc catggcggtg ctggccaaga
    11461 ctacgccgat agagccggtc tttctggaga tcctcgacct cgtcgccctt caggacgagc
    11521 cgcacgccat cccggccttg atgcgggcgc ttgtggatct ggcgcgggcg cggggcgcgg
    11581 ccaaggttcg gctgcaggtc gtcaatgagg agctcaagcg ccgcttggga ccgtgggctc
    11641 tcgccgcacg ccgcgagggt ggatggggcc acggccacat tcgttttcgt ccgggcgggg
    11701 aaagcctcca gacatggtcg ccgacgccct tcgatgggga ccacgccttt tgccagcgcc
    11761 cggcgccggc tcggggcacg ccgatcaccg gaaaccgccg tcccgcagtc tgactagacg
    11821 gaagggcggg ccttcagttc gtcgcggatt tcggcgagca gcacttcggt cgtggtcggg
    11881 gccggcggcg gcgctgcgac ctcggggtgg gcggcttcct ggcgccgcag cttgttgatg
    11941 gccttgacca ccatgaagac tacgaaagcc acgatagcga actgaatgac ggtgttcaaa
    12001 aaggcgccgt actggatcga aaccttctcg atcgcctcgg tcgccggatt ttcagcccgc
    12061 aggacccatt ccagtttgga gaagtcgatg cctccggtca gcagcccgat cggcggcatg
    12121 acgatctgat cgaccagact tttgacgatg gagttgaagg cggcgccgat gatcacgccg
    12181 acagcgaggt cgacgacgtt gcccttgatg gcgaattcgc gaaactcgga aacgaggctc
    12241 ataggcatcc ttccttggcg tctatcaggc gtcgcccgag gcgttgagac gctcgcgcag
    12301 ctccttgccg ctcttgaaga aggggacgga cttggccggg acgtccaccg tctcgccggt
    12361 gcgtgggttg cggccggcgc gggcaggacg tgagcgcacc gagaaggcgc cgaatccgcg
    12421 cagctccacg cgaccaccgt cggccatggc ctcggtcatc cgacccagca cgacgttgac
    12481 cacccgttcg acctcagcat gagtgaggtg ggtgttctct gcggccagtt tttcgatcag
    12541 ctcagacttg atcatctcgt ttgaggcccc cgcctgtctg ttgtgccctt ccagaggctt
    12601 tgattgccct cgtttaagcg acggcgtcgg caccctagcc ttggcgtggc tggcgaaaaa
    12661 gccccgatgg cgtaaatcgg gccagcaagg tgattaatag tacgctaggc gacagagcgt
    12721 cggttggtgg gcaaagaaaa aggcggccgg atcgctccgg ccgcctccat tctcagtggt
    12781 tcaggacgag attagtcctg tttggctttc tcgcgcagag ctgcgccgag gatgtcgccg
    12841 agcgaagcgc ccgagtccga agacccgaac tgttcgatgg cgtccttctc gtcggccatt
    12901 tccagagcct tgatcgagac ggcgatacga cgcgtggcct tgtcgatcga cgtgatctgg
    12961 gcgtcaacgc ggtcgccgac ggcgtaacgc tcaggacgtt gctcgttgcg gtcgcggctc
    13021 aggtccgact tgcgcacgaa gctggtgacc ggtgcgtcat cgtcgccgaa cttgacttcg
    13081 atgccgccgg tttcgatcga ggtgacggtg acggtgacgg tctgaccctt cttgtacagg
    13141 tcgccttcgc cgacgggatc gccgccgagc tgcttgatgc ccagcgagac acgttccttc
    13201 tcgacgtcga cgtcgagaac cttggccttg accatttcgc ccttgcgata gcgctggatg
    13261 gcttcttcgc cggagacgct ccagtccaga tcggacaggt gcaccatgcc gtcgatgtcg
    13321 ttgtccaggc cgatgaacag gccgaactcg gtggcgttct tgacttcacc ctcgacggtc
    13381 gagccgatcg ggtgcgaggc cacgaaggca tcccacgggt tgtcctgggc ctgcttcagg
    13441 cccagcgaga tgcggcgctt ggaggcgtcg acgtccagca cgaccacgtc gacttcttgc
    13501 gaggtcgaga cgatcttgcc ggggtggacg ttcttcttgg tccaggacat ttccgagacg
    13561 tgcacgaggc cttcgacacc ggcttccagc tccacgaagg cgccgtaatc ggtgatgttg
    13621 gtgatgcgac cggtgtactt ggcgccgacc gggtatttgg cttcaacgcc gtcccaggga
    13681 tcggactgca gttgcttcat gccgaggctg atgcgttgcg tgtcggggtt gatcttgacg
    13741 atctggacct tgacggtgtc gccgacggcg aggacttgcg aggggtgcga gacgcgcttc
    13801 cacgacatgt cggtgacgtg cagcaggccg tcgatgccac ccagatccac gaacgcgccg
    13861 tagtcggtga tattcttgac gacgccgtcg cggacttcgc cttcttgcaa ctggccgacg
    13921 agctcggtgc gttgttcagc acgggcttct tccaggatcg cacgacgcga gacgacgatg
    13981 ttcccgcgcg ggcggtccat cttcaggatc tggaacggtt gttccttgcc catgagcggg
    14041 ccgacgtcgc ggaccggacg gatgtcgact tgcgagccgg gcaggaacgc ggaagcgccg
    14101 cccaggtcga cggtgaagcc gcccttgacg cgaccgacga tggcgccgtt gaccggcacg
    14161 ccttcggcga acacgacttc cagacgggtc caggcttctt cgcgacgcgc cttgtcgcgg
    14221 ctgatgacgg cttcgcccag agcgttttca acgcgttcca ggtaaacctc gacgtcgtcg
    14281 ccgaccttcg ggatcacgga gccttcgccg atgccgaact cacgggcggg gatacgacct
    14341 tcggtcttca gaccgacgtc gatgatgatg atgtctttct cgatgccgac gacgcggccg
    14401 tgaacgacct ggccttcacc gaggtcgcgg ccttgcagtt gctcgtcgag cagggctgcg
    14461 aaatcgtcac gtgaggggga gtgggtatca gacatgaagc tctggtgttt ccgaatgggc
    14521 tatggcgcgc gaccgaatgg ccccgcgcga ggtgaaggag aagttgggaa atcagtctca
    14581 acgaagcgaa cttcagctaa ccagctgaac cagaacgccc gcggaagccg aggatgggag
    14641 cgttggattt gcgatgtcgg gtcagagacc gtgtttcgcg cgcgccgcct cgacgatacg
    14701 gcgggccgca tcgaaggccg tctctatatc catttccgtc gtatccagca agaccgcgtc
    14761 ctgagcctga atcatggggg ccgatccacg ccctgcatca cgttcgtcgc gacggacgat
    14821 gtcggccagc atgtcttcga acgagatgtc gtcgccgcgc gccgtgagct gtttccagcg
    14881 acgcgtggcg cgggtctcag gcgtcgcggt gatgaacagt ttggccgggg cgttgggcgc
    14941 aatgactgtg ccgatatcgc gtccgtccag cacggcgccg cccggttggg aggcaaacgc
    15001 ctgttgcagg tccagcaagg ccgcccgcac gccgggatgc cccgcgaccc ggctggccgc
    15061 ctcacccgct tcgcccgtcg tcaggcgcgg atcgtccgac aggccgttgg cgtcgagagt
    15121 gcgcgctgcg atttcggcag cggcttcgtc gtcgagggat cccccggcgt cgagaacctt
    15181 gaggccgacg gcccggtaaa gcagcccggt atccagaaac ggcaacccaa acgtcgtagc
    15241 cagccgcgca gcgacggtgc ccttgcccga cgcggcggga ccatcgacag cgatgacgag
    15301 aggcgcggtc attcggctga tgcgacgtgg ccgcccagcc cgaccatcat ctccgcgaag
    15361 cccgggaagc tggtggcgat catgtccggc tcgtcaacag tcaccggcgc cgcagcggcg
    15421 aggccgagca ccagatggct catggcgatg cgatgatcat gggcggtgtg tacggttgcg
    15481 ccgccgggga ccgatcctcc ggtgacgagg aaaccgtccg gctcctcctc gaccaccacg
    15541 ccacaggcac gcaacccatc gaccatcagg cggatgcggt cgctttcctt gacccgcatc
    15601 tcgccaacgc cacgcatgac cgtcacgcca tga
//
LOCUS       DASVTM010000354         4161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_88464, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000354 DASVTM010000000
VERSION     DASVTM010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88464"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VF633_09625"
     CDS             complement(<1..370)
                     /locus_tag="VF633_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX8471360.1"
                     /translation="MRAASITDYRELARRRLPLFLFDYIDGGSYAETTLRRNVSDLQD
                     IALRQRVLKDVSDLTLETKLFGETLSLPVVLAPVGLTGMYARRGEVQAAKAAASRGVP
                     FTLSTVSVCAIEEVAPAIDRP"
     gene            complement(376..1137)
                     /locus_tag="VF633_09630"
     CDS             complement(376..1137)
                     /locus_tag="VF633_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8471361.1"
                     /translation="MLLDNKVVLVTGGSRGIGAAVAIECARHGADVAIHSNRGGEAAA
                     EVIAAIEALGRRAVHVEGDVAQHESAAAFVAAAVEAFGRVDVFVSNAGICPFHAFLDM
                     PPETLKRTMEVNLHGAYFMTQAAANQMVKQGDGGSIIAISSISALVGGEYQTHYTPTK
                     AGVHSLMQSCAIALGRHGIRCNSVLPGTILTDINKDDLADPAKRQYMEGRIPLGRLGQ
                     PEDIGGPVVFLASDMAKYVTGAALLVDGGMFVNLQ"
     gene            complement(1160..1954)
                     /locus_tag="VF633_09635"
     CDS             complement(1160..1954)
                     /locus_tag="VF633_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX8471362.1"
                     /translation="MGQTASIDTVEPEEPAKASGSQTLLRGLDVIDALIDGPLSLSDL
                     SEKLELTRSTTHRLAAALVDRRLLSFRPREGYTLGPKLLELGHAASHQMHLPRIARPW
                     LEQLSAATEDTVHLGVLDGSHALYLDKVSGRRRINIGSRLGERHPIASTGLGKALILD
                     KSEPEWMALYSPEGTPFDSTARAVWLERMRGYAAKGYAFDLEENEDQIRCVAAPVRGV
                     DGKVVAAVSVSSAAQYMSDARMAELTKTVTDAANAISRDLGWSPKP"
     gene            2092..3285
                     /gene="rhmD"
                     /locus_tag="VF633_09640"
     CDS             2092..3285
                     /gene="rhmD"
                     /locus_tag="VF633_09640"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HEX8471363.1"
                     /translation="MSRLPKIKHVRAFVVKNDGSGGGADYHDQGDGHWIDDHIATPMS
                     KYPEYRQSRRSFGINVLGTLVVEIEAENGVVGFAVTTGGEPAAYIVEKHLARFLEGRD
                     PSEVEKIWDQMYFSTQYYGRKGLVVNAISGVDLALWDLLGRLRQEPVFAMLGGPVRDE
                     LTFYATGARPDKAKELGFIGGKMPLHHGPAEGEEGLHKNLAELEAMRNACGDDFWLMF
                     DCWMALDLNYATRLAHEAHKLGLKWIEEALSPDDYWGYQALKKNVPKGMLVTTGEHEA
                     TRWGFRMLLEMECCDIIQPDVGWCGGVTELIKISALADAKGVLMIPHGSSVYSYHFVV
                     TRHNSPFAEFLMMAPGADEVVPMFHPQLIGEPVPVNGRLKVPDTPGFGVELNRDIALH
                     RPYAR"
     gene            3301..4152
                     /locus_tag="VF633_09645"
     CDS             3301..4152
                     /locus_tag="VF633_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8471364.1"
                     /translation="MKLLRFGPKGQEKPGLMDDEGCIRDLSGHIADLTPDHLSPEGLK
                     KLAAIDTGSMPVVEGSPRYGVPVAGSRKFIAIGLNFADHAAESNLPIPSEPVMFTKAI
                     TCLNGPNDDVVIPRGSLKTDWEVELGVVIGKTASYVTKEEALDHVAGYVLINDVSERA
                     FQTERGPTWDKGKGCDTFGPVGPWLVTTDEVGDVQNLAMWLDVNGKRVQTGNTNTMIF
                     GVAEIVSYLSEFMTLEPGDLITTGTPPGVGLGQKPEPWYLKAGDTVRLGIEKLGEQGQ
                     TFVAWSR"
BASE COUNT          767 a         1326 c         1339 g          729 t
ORIGIN      
        1 tcggacggtc tatcgcgggc gcaacctcct cgatggcgca gaccgagacg gtcgacaggg
       61 tgaatggcac gccacgcgac gccgccgcct tcgccgcctg aacctcgccc cgacgcgcgt
      121 acatgccggt caggccgaca ggcgcgagga cgacgggaag gctcagcgtc tcgccgaaca
      181 gcttcgtctc gagcgtcaga tcggacacgt ccttcagcac cctctggcgc agggcgatgt
      241 cctgcaggtc gctgacgttc cgacgcagcg tcgtctcggc ataggaaccg ccatcgatat
      301 agtcgaacag gaacaggggc agacgtcgac gcgccagctc acgatagtcg gtgatggagg
      361 cggcgcgcat ggcccctact gcaggttcac gaacatgccg ccatcgacca gcagggccgc
      421 gccggtgacg tatttcgcca tgtcggacgc caggaatacg accggtccgc cgatgtcctc
      481 aggctggccg agacgaccca gagggatgcg gccttccatg tattggcgct tggccggatc
      541 ggcgaggtcg tccttgttga tgtcggtcag gattgtgccc ggcaggactg agttgcagcg
      601 gatgccgtga cgccccagcg cgatggcgca ggactgcatc aggctgtgaa cccccgcctt
      661 ggtcggtgtg tagtgggtct ggtactcgcc gccgacgagg gcgctaatcg agctgatggc
      721 gatgatggag ccgccatcac cttgcttcac catctggttg gcggcggcct gggtcatgaa
      781 ataggccccg tgcagattga cctccatcgt tcgcttcagc gtctcgggcg gcatgtcgag
      841 gaaggcgtgg aacggacaga tgccggcgtt tgaaacgaac acgtccacgc ggccgaaagc
      901 ctcgacggcg gcagcgacga aggctgcagc gctctcgtgc tgggccacgt cgccttcgac
      961 atggacggcg cggcggccga gcgcctcgat ggcggcgatg acctcggcgg ccgcctcccc
     1021 gccccggttc gagtggatcg ccacgtccgc gccgtgacga gcgcattcga tggcgacagc
     1081 cgcgccgatg ccgcgtgatc ctccggtgac gaggacgacc ttgttatcaa gcagcatggg
     1141 aactccggcg acggtggatt tacggtttgg gtgaccagcc gagatcgcgg ctgatggcgt
     1201 tggctgcgtc ggtgacggtc ttggtcaact cggccatgcg ggcgtcgctc atatactggg
     1261 cggcgctgga tacgctgacg gcggcgacga ccttgccgtc gacaccgcgc acgggggcgg
     1321 cgacgcagcg gatctggtct tcgttctcct cgaggtcgaa ggcatagccc ttggcggcgt
     1381 atccgcgcat ccgttcgagc cagaccgcac gcgcagtcga gtcgaaaggc gtgccttcag
     1441 gcgagtagag cgccatccat tccggctcac tcttgtcgag gatcagcgcc ttgccgaggc
     1501 cggtcgaggc gatcggatga cgctctccca gtcgcgagcc gatgttgatc cgacggcggc
     1561 cagagacctt gtcgagatag agggcgtggg agccgtccag cacgcccaga tgaaccgtgt
     1621 cctcggtggc ggcggacagt tgctcgagcc agggccgagc gatccgcgga agatgcatct
     1681 ggtggctggc tgcatgaccc agttcgagca gtttgggccc gagggtgtag ccttcgcgcg
     1741 gacggaagga gagcagacgt cgatcgacca aagccgccgc aagccgatgc gtcgtgctgc
     1801 gcgtcagctc gagcttctcg gacaggtccg aaagcgacag cggaccgtcg ataagcgcat
     1861 ctataacgtc gagcccacgc agcagggttt ggctgccgga agccttggcc ggttcttccg
     1921 gttctacggt gtcgattgac gcagtttgtc ccatcacgta atcacctgtt tcagaatgta
     1981 gcaaaacgcg caagtcggac ggcgattgcc ggttcagttt gacgcgacac gcggagaaac
     2041 accagtgtca tcccgaaata tgggaacccg tagtcaagcg gcgtttcgcg catgagtaga
     2101 ttgccgaaga tcaagcacgt ccgcgccttt gtcgtgaaga acgacgggtc gggcggcggc
     2161 gctgactatc atgaccaagg cgacggccac tggatcgacg accacatcgc cacgccgatg
     2221 tcgaaatacc ccgagtaccg ccagagccgc cgcagcttcg gcatcaatgt gctcggtacg
     2281 ctggtggtcg agatcgaagc cgagaacggc gtcgtcggtt tcgccgtgac gaccggtggc
     2341 gagcccgccg cctatatcgt cgagaaacac ctggcccgct tcctcgaagg tcgcgatccg
     2401 tccgaggtcg agaagatctg ggaccagatg tatttctcga cccagtacta tggccgcaag
     2461 ggtctggtcg tgaacgccat ctccggcgtc gatctggcgc tttgggacct gttgggccgt
     2521 ctgcgtcagg agccggtctt cgccatgctc ggtggccccg tgcgcgacga actgaccttc
     2581 tacgctaccg gcgcgcgccc ggacaaagcc aaggaactcg gcttcatcgg cggcaagatg
     2641 cccctgcatc acggccctgc cgaaggtgag gaaggcctgc acaagaacct cgccgaactc
     2701 gaagccatgc ggaatgcgtg cggcgacgac ttctggctga tgttcgactg ctggatggcg
     2761 ctggatctga actacgccac ccggcttgcc catgaggcgc acaagctcgg cctcaaatgg
     2821 atcgaggagg cgctcagccc cgacgactac tggggctatc aggcgctgaa gaagaacgtc
     2881 cccaagggca tgctcgtcac caccggtgag catgaggcca cccgctgggg cttccgcatg
     2941 ctgctggaga tggagtgctg cgacatcatc cagccggacg tcggctggtg cggcggcgtc
     3001 accgagttga tcaagatttc ggccctggcc gacgccaagg gcgtgctgat gatcccgcat
     3061 ggatcgtcgg tctattccta ccatttcgtc gtcacgcgcc ataacagccc gttcgccgag
     3121 ttcctgatga tggctcccgg cgccgacgag gtcgtgccga tgttccatcc gcagctgatc
     3181 ggcgagcccg tgcccgtgaa cggccgtctt aaagtccccg atactcccgg cttcggcgtc
     3241 gaactgaacc gcgacatcgc cttgcatcgc ccctacgcgc gctaagaaaa ggtcttcaaa
     3301 atgaaactgc tgcgtttcgg ccccaagggt caggaaaagc ccggtctgat ggacgacgaa
     3361 ggctgcatcc gcgacctgtc gggacacatc gccgacctca cgcccgacca cctttcgccc
     3421 gaaggcctga aaaagctggc cgcgattgac accggttcca tgcccgtcgt cgaaggctcg
     3481 cctcgctatg gcgtgccggt cgccggcagc cgcaaattca tcgccattgg cctcaacttc
     3541 gccgaccacg ctgcggaatc gaacctgccg atcccttcgg aacccgtcat gttcaccaag
     3601 gcgatcacct gcctgaacgg tcccaacgac gacgtcgtca ttccgcgcgg ctccttgaag
     3661 accgactggg aagttgaact cggcgtggtg atcggcaaga ccgcctccta cgtgaccaag
     3721 gaagaggcgc tcgaccacgt cgccggctat gtcctgatca acgacgtttc ggagcgcgcc
     3781 ttccagaccg agcgtggccc gacctgggac aagggcaagg gctgcgacac cttcggtccc
     3841 gtcggcccct ggctggtcac gacggacgaa gtcggcgacg tccagaacct ggccatgtgg
     3901 ctcgacgtca atggcaagcg ggtccagacc ggcaatacga acacgatgat cttcggcgtc
     3961 gccgagatcg tctcctacct gtccgagttc atgacgctgg aacccggcga cctgatcacc
     4021 accggcaccc ctccgggcgt cggcctcggc cagaagcctg agccctggta cctcaaggcg
     4081 ggggacactg ttcgtctcgg catcgagaag ctcggcgaac aaggccagac cttcgtagcg
     4141 tggtcgcgat gagcgtctat t
//
LOCUS       DASVTM010000355         5490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_990037,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000355 DASVTM010000000
VERSION     DASVTM010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990037"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF633_09650"
     CDS             <1..290
                     /locus_tag="VF633_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471365.1"
                     /translation="SHGLVYGFDEVEGGEVIVDGHVLARIRRGEVRKSLDVMAPDAEM
                     VTLRLMFADARYPEFELALWNATAPAQTGSPAEALRLGRRWLSHIEALLKP"
     gene            366..1274
                     /locus_tag="VF633_09655"
     CDS             366..1274
                     /locus_tag="VF633_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiG"
                     /protein_id="HEX8471366.1"
                     /translation="MARLFDAYVIADWTAAEGKKLGENSVWIGVAKRDVRFRLYTETH
                     NVATRAEGEALLTSILADHRKRGDRVLVGFDFSLGYPVGTAKRLALTATPAWSALWKF
                     IASNVVDKADNTNNRYQVAAKMNRLMTDQAWPFWGAPANQAQRWLTTTKPPVGSSPDI
                     PEYRATENAVRKGRLQPKSNWQMHGAGAVGGQTLVGIPAVRRLLESLGSSAAVWPFGT
                     GWRQLDAADVEPLSVLVVEVWPSMWPTTAAEGEFKDQAQVRTTAMAFAEMDDKGDLAK
                     AFGPPKNATPELIEQVEQEEGWILGV"
     gene            complement(1429..4740)
                     /locus_tag="VF633_09660"
     CDS             complement(1429..4740)
                     /locus_tag="VF633_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471367.1"
                     /translation="MLASTVFTTVLALGAPALAQTQALTAEPVAGAQDETTQVGEIVV
                     TGSRIRRDATNAPTPLIQVSQEQLLSTGQSTVIDYLATIPALSNSVVPSDTTGTGLGD
                     GGLSLPNLRSLGSNRTLTLVDGRRHVGSSGGSLSVDIDTIPRLLIENIEIVTGGAASV
                     YGADAVSGVLNFILRKDFEGLEVDANYGMINQDGQANRRISALIGKNFFDDRLNVYAH
                     AEYEKVDEVQSLDIDWLRRAPVRIGVDADPTTAPSDGILDARLFTGVSRMDNPRWGQT
                     TLVNVQQPSPLNNPNVPYDDCFPGGSFSYAPACFAVTPGKTFWYDGTSARLANFGTRV
                     GNTGANRPYNIGGDGENPAEFSTGSRVPRSESQRYQVGASFKVTDNITAYGEAKYVTE
                     DTFDVGQPTFFDIDLVNSYGPGEVNPIYSTNNFDLRWTDNAFLPQNVKDAIRTNLVTP
                     YDAPTATTPGGAGTPFLLQQARHSLFGPDRSQNNTRDLQRYVAGVRGDLGDFAFVKDI
                     NFDIGYTYGKVEVENRERGVDSQRFALAIDSVVDTAGIVNGTPGQIVCRAQLIAKQGG
                     VVTDYNTGTDNYLGDLRDTAYGQAAIAQCAPLNVFGKGNQSEAALNYVDATVGVTEEN
                     EQQQGIASISGNLWDFWGAGPIGVAIGGEYRKETTSATGRDRDTAGRLLFLNSGPDFP
                     EASYESKEVFAELSVPLFRDSFLGEYAELSGSYRYSDYSTVGGADVYGVNLVYRPIRD
                     ITFKTSYNTSIRVPDLGENFAPNSQTFANGIVDPCTTANIAAVNDAETRANRVANCTA
                     LAGAQGLTFDFAGLTPENTDDFRPNYTSGIAGVLGGNPNLKPEESDSFTFSAVLRPRF
                     IPNFSLVLDYYEINIDDVISAVSADTAAANCVNGPTLNTDACSTIFRNNPAIPFGVGA
                     PAGDPIGGFIERSFNYAALKTRGLDFTARYMIDTEETFGRNFGQLDYSIAGSWLITQQ
                     QYLNEADASDYDELAGTLYYPRVRFTSSLTWTPNETWSVNWTADWQSAQNIVYARDFV
                     NNADSREVGQMDTGNFVRNDFTLRYNVTDDLSLRTGVVNAFDAEQAPYLGTTLYSNFD
                     PYGRRFFVGLNWKAW"
     gene            complement(5102..>5490)
                     /locus_tag="VF633_09665"
     CDS             complement(5102..>5490)
                     /locus_tag="VF633_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX8471368.1"
                     /translation="LDAIASGGELARFALAMKAALAGREGQRQPVMIFDEVDQGVGGA
                     VAEAVGQRLKRLSNGAQVLVVTHSPQVAARGHAHWKVMKADRDGATTTTVIALNDARR
                     QEEIARMLSGAVITDEARAAAKALIG"
BASE COUNT         1024 a         1614 c         1786 g         1066 t
ORIGIN      
        1 gcagccacgg tctggtctac gggtttgacg aggtcgaagg cggcgaagtc atcgtcgacg
       61 gtcatgtcct cgcccgcatc cgacgcggcg aggtcagaaa gtcattggac gtcatggcgc
      121 ctgacgctga gatggtgacg ctgcggctga tgttcgcgga cgctcgatac cccgagttcg
      181 aactggcctt atggaatgca acggccccgg cccagacggg ctcacccgcc gaggcgcttc
      241 gtctcggccg tcgctggctc tcgcacatcg aagcgctcct gaagccctga tcgctctttc
      301 ggcggccgcg tgggacgagt agaaccccgc attccttccg tgcccgagtt tttgaggagc
      361 cccccgtggc ccgccttttc gacgcctacg taatcgctga ctggaccgcc gccgagggca
      421 agaagctcgg cgagaattcc gtgtggatcg gggtggccaa acgcgacgtt cgctttcgcc
      481 tgtatactga gacccacaac gtcgcaacgc gggccgaggg cgaggcgctg ctgacttcaa
      541 tccttgctga tcatcgcaag cgcggggacc gggttctggt cggtttcgac ttctcgctcg
      601 gctatcctgt cggcacggcc aagcgcttag cactgaccgc gacgccagcc tggtcggctc
      661 tgtggaaatt catcgcctcg aacgtcgtcg acaaggcgga caacaccaac aaccgctatc
      721 aggtcgcggc caagatgaac cgcctgatga ccgatcaggc ctggccgttc tggggcgccc
      781 ccgccaatca ggcgcagcgc tggctgacca cgaccaagcc tccggtcggg tcatcccctg
      841 acattcccga gtaccgcgcg acggaaaacg ccgtacgcaa aggccggctg cagcccaagt
      901 ccaattggca aatgcacggc gcgggcgctg tcggcgggca aacccttgtc ggcattccgg
      961 ccgtgcgtcg tttgttggaa agcctcggct catcggctgc cgtctggccg ttcggaacag
     1021 gctggcgtca gcttgacgcc gccgacgtgg agccgctgtc ggtgctggtc gtcgaagtct
     1081 ggccgtccat gtggcccacg accgccgccg agggcgagtt taaggatcag gctcaggtcc
     1141 gcacgaccgc gatggccttc gccgagatgg acgacaaggg cgatctggcc aaggccttcg
     1201 ggccgccgaa gaatgcaacg cccgagctga tcgagcaggt cgaacaggaa gaaggctgga
     1261 tcctcggcgt ctgatccagg cgtcggcagt ccagataaca aaacggcccg actgacaggg
     1321 ggcgctgtcg gtcgggccgt ctggttttgg gtctcatcga cccatccgtc ccacctctcc
     1381 gtgggccgga aaatcaacgg gaggcggcga gccgcccccc gtcagccgtt accaggcttt
     1441 ccagttcaga ccgacgaaga agcggcggcc gtaggggtcg aagttcgagt acagggtcgt
     1501 gccgaggtaa ggcgcctgtt cggcgtcgaa cgcgttgacc acaccggtcc gcagcgacag
     1561 gtcgtcggtg acgttgtagc gcaaggtgaa gtcgttccgg acgaagttgc ctgtgtccat
     1621 ctggccgact tcacgagagt cggcgttgtt cacgaagtcg cgggcgtaaa cgatgttctg
     1681 cgccgattgc cagtcagccg tccagttgac gctccaggtc tcgttagggg tccaggtcag
     1741 agacgaggta aagcggacgc gcgggtagta gagagtgcct gctagctcat catagtccga
     1801 cgcatcagcc tcgttcaggt actgctgctg tgtgatcagc caggaaccgg caatcgagta
     1861 atccagttgg ccgaagttgc ggccgaaggt ttcctcggtg tcgatcatgt agcgggctgt
     1921 gaagtccaga ccgcgggtct tgagcgctgc gtagttgaac gagcgctcga tgaagccacc
     1981 gatcggatcg cctgccggag cgccgacgcc gaatgggatg gcggggttgt tacggaagat
     2041 ggtcgagcag gcgtcggtgt tcagcgtcgg accgttgacg cagtttgccg ccgccgtatc
     2101 agccgacact gccgagatga cgtcgtcgat gttgatctcg tagtagtcga gaaccaggct
     2161 gaagttcggg atgaagcgcg gacgcagcac cgccgagaag gtgaacgagt cggattcttc
     2221 cggcttcagg ttcgggttgc cgcccagcac gccagcgatg cctgaggtgt agttcggacg
     2281 gaagtcgtcg gtgttctcgg gcgtcagtcc ggcgaagtcg aaggtcaggc cctgagcgcc
     2341 cgcgagcgcc gtacagtttg cgacccggtt ggcgcgggtt tcagcgtcat tgaccgccgc
     2401 gatgtttgcg gtggtgcacg gatcgacgat gccgtttgcg aacgtctggc tgttgggcgc
     2461 gaagttctca cccaggtcag gcacgcggat tgaggtgttg tagctggttt tgaacgtgat
     2521 atcgcggatc gggcgataga cgaggttcac accgtaaacg tcggcgccgc ccacggtcga
     2581 atagtcggag tagcggtagg aaccactgag ctcggcatac tcgcccagga agctgtcgcg
     2641 gaacaatggc accgaaagct cggcgaagac ttccttgctc tcatagctgg cttccgggaa
     2701 gtccgggccg ctgttcagga acagcagacg gccagccgta tcgcggtcgc ggcccgtggc
     2761 cgaggtagtt tccttgcggt attcgccgcc gatggcgacg ccgatcggac ctgcacccca
     2821 gaagtcccac aggttgccgg agatcgatgc tatcccctgc tgctgttcgt tctcttcagt
     2881 gaccccgacg gtggcatcga cgtagttcag cgcggcttcg ctctggttgc ccttgccgaa
     2941 gacgttcaga ggcgcgcact gggcgattgc agcctgacca taagcggtgt cacgcaggtc
     3001 gcccaggtag ttgtcagtgc ccgtgttgta atccgtgaca accccgccct gcttggcgat
     3061 cagttgagca cggcatacga tctgacccgg cgtgccgttg acgatgcctg cggtgtcgac
     3121 aaccgagtcg atggccaagg cgaagcgttg gctgtcgacg ccgcgttcgc gattttcgac
     3181 ctcgaccttg ccgtaggtgt agccaatgtc gaaattgata tccttgacga aggcgaagtc
     3241 gccaagatcg ccacgaacac cggcgacgta gcgctgcaag tcgcgtgtat tgttttgcga
     3301 ccggtcagga ccgaacaggc tgtggcgcgc ttgctgaagc aggaacggcg tccctgctcc
     3361 acccggagtg gttgccgtcg gcgcgtcata gggggtcacg agattagtgc ggatcgcgtc
     3421 tttgacgttc tgcggaagga aggcgttgtc ggtccagcgc aggtcgaaat tgttggtcga
     3481 gtaaatcggg ttcacttcgc cggggccgta ggaattcacc aggtcgatgt cgaagaacgt
     3541 aggctgtccg acgtcgaagg tatcttccgt gacgtacttc gcttcaccgt aggcggtgat
     3601 gttgtcggta accttgaagc tggcgccgac ctggtagcgc tgcgactccg agcgggggac
     3661 gcgcgaaccg gtcgagaact cggccggatt ctcgccgtca ccgccgatat tataggggcg
     3721 gttggcgccg gtgttgccga cccgggtgcc gaagttggcc agacgggcac tggtgccgtc
     3781 ataccagaaa gtcttgccag gcgtgactgc gaagcaagcc ggggcgtagc tgaagctgcc
     3841 gcccgggaaa cagtcgtcgt aaggcacgtt cgggttgttc agcgggctcg gctgctgaac
     3901 gttgaccaac gtcgtttggc cccaacgcgg attgtccata cggctgacgc ctgtgaacag
     3961 tctggcgtcc agaatgccgt cgcttggagc ggtggtcgga tccgcgtcta caccgatgcg
     4021 aaccggggca cgacgcagcc agtcgatatc gagcgactgg acttcatcga ccttttcata
     4081 ctcggcgtga gcatagacgt tcagacgatc gtcgaagaag ttcttgccga tcagggctga
     4141 gatgcggcgg ttagcttggc cgtcctgatt gatcatgccg tagttggcgt cgacctcaag
     4201 accttcgaaa tccttgcgca ggatgaagtt cagcacgccc gagacggcgt cagcgccgta
     4261 aaccgaggcg gcgccgccag tgacgatctc gatgttctcg atcagcaggc gcggaatggt
     4321 gtcgatatcc accgaaagcg aaccgcccga cgagccgaca tgacgacgac cgtctacgag
     4381 ggtcagggtg cggttggagc cgagcgaacg caggttgggc agcgacagcc ccccatcacc
     4441 aaggcccgtt ccggtcgtgt cggacggcac cactgagttc gaaagcgccg ggattgtggc
     4501 caggtaatcg ataaccgtac tttgacccgt gctgaggagt tgctcctgtg acacctggat
     4561 cagtggggtg ggggcatttg tggcgtcgcg gcgaatacgc gagccggtga cgacaatttc
     4621 cccgacctga gtggtttcgt cctgcgcgcc tgcaactggc tctgcggtga gagcttgagt
     4681 ttgggcgagg gcgggtgcgc cgagcgcgag gacggtcgta aatacagtgg aagctaacaa
     4741 atgtttacgc ataacaacca ttttcaaaaa gagcgtacgg ctaagctaca tccatgacgc
     4801 agagacgccg cgatgcggac acaaacaatt cacacgcgtc tcgcgtcaag cgatgcagtg
     4861 caccaacaca aaatcgatca gcgttattga actcggcgtg ctttttatgt gtcaatagtc
     4921 gaaatgctga gtttcgtatg aaaaacgtct gttgttggcg gaacttcact ggaaatcgag
     4981 cgttcgcaca tttgtgcgat ccggggggcg ttgcggctcc cgagcgtacg gttaagcctt
     5041 ttgtaaattg cagcgcccga aggcgacctg gccgttcaac gccactgtcg tcgtcagctt
     5101 atcagccgat cagcgccttg gcggccgctc tggcctcgtc ggtgatgacg gcgcccgaaa
     5161 gcattcgtgc gatctcttcc tgacgacgag cgtcgttcag ggcgatgaca gtggtggtag
     5221 tcgcgccgtc acggtcggcc ttcatgacct tccagtgagc atggccacgc gcagcgacct
     5281 ggggactgtg ggtgacgacc agaacctgag cgccgttcga cagccgcttc agccgctggc
     5341 ccacggcctc agcgaccgcg ccgcccacgc cctgatcgac ctcgtcgaag atcatgacag
     5401 gctgccgctg accttcacgt cccgccaatg ccgccttcat cgccagagca aagcgcgcca
     5461 attccccgcc tgaagcgata gcgtccagag
//
LOCUS       DASVTM010000356         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_302372,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000356 DASVTM010000000
VERSION     DASVTM010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302372"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1155
                     /locus_tag="VF633_09670"
     CDS             175..1155
                     /locus_tag="VF633_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX8471369.1"
                     /translation="MLTRRRAGTAALMCLLTLPVTAMAQSGYPSKPIRVVVPFAAGST
                     TDIIARAITDKMSASMGQQLLVDNRAGASGTIGQQAVATAAPDGYTVLIHSSSHTVSP
                     STFAKLPFDTLQDFAGVTPIASVPNALVVSPTKNIKNLKGLLDAAKASGNDGLNYASA
                     GQGSATHLNAEKFRVAANIKATNIPFKGSAEAVTEVMGGRVDYYFSPIAPVIGQIKSG
                     QLVALAVGSPRRSSALPDVPTTAEAGVRGSEFNFWIGMMVPAKTPRDLIDRLNAEVLK
                     ALASPDVKERFLQLGVDPWTMKPADFDAYIKTEVVSNAKLVKDAGLTPTQ"
     gene            1192..>1798
                     /locus_tag="VF633_09675"
     CDS             1192..>1798
                     /locus_tag="VF633_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8471370.1"
                     /translation="MPTQRLGLIMHGVTGRMGMNQHLIRSIVAIRDQGGVTLSNGDKV
                     MPDPILIGRNAEKIEALARLHRIERWGTDLKAALGDDSDAVFFDAGTTQMRPSLLEDA
                     LRAGKHVYCEKPIATDLDQAVKIAKIAQSSGLKHGAVQDKLFLPGLRKLDMLRRAGFF
                     GRMLSVRLEFGYWVFEGDLQPIQRPSWNYRAEDGGGMILDMM"
BASE COUNT          315 a          619 c          569 g          295 t
ORIGIN      
        1 gcttccttgg tgggcggcgg gctcgggccc tgggtgtgac cacgcctccg ctgcgccctt
       61 cgcgttgtgg caggctcgtg aggggagtag ggcctgcgcc cttgacgccg gccgagtccg
      121 gtccctaaac tgaacaattc actgagtggt gaattgcccc atctggagtt ccacatgttg
      181 acccgtcgtc gtgccggtac cgccgccctg atgtgcttgc tgaccttgcc cgtgaccgcg
      241 atggcgcaga gcggttaccc ctccaagccc atccgggtcg tggtgccgtt cgccgccggc
      301 agcaccacgg acatcatcgc ccgcgccatc acggacaaga tgtccgccag catgggacag
      361 cagctcctcg tcgacaaccg ggccggagcc agcggcacga tcggccagca ggccgtggcg
      421 accgccgccc ccgacggcta caccgttctg atccattcct cgtcgcacac ggtgagtcct
      481 tcgacgttcg ccaagcttcc gttcgacacc ctgcaggact tcgccggggt gacgccgatc
      541 gcctcggttc cgaacgctct ggtggtttcg cccacgaaga acatcaagaa cctgaagggg
      601 ctgctggatg ccgccaaggc gtcgggaaac gacgggctga actacgcctc cgccggccag
      661 ggaagcgcga cgcatctgaa cgccgagaag ttccgggtcg cagccaacat caaggcgacc
      721 aacatcccgt tcaagggctc ggccgaagcg gtgacggagg tgatgggcgg ccgggtcgac
      781 tactactttt ccccgatcgc ccccgtcatc ggccagatca agtcgggcca gctggtcgca
      841 ctcgccgtcg gctctccccg ccgctccagc gctctgcccg atgttccgac gaccgcggaa
      901 gccggcgtgc ggggctccga gttcaacttc tggatcggca tgatggtccc ggcgaagacg
      961 cctcgcgacc tcatcgaccg cctgaacgcg gaagtcctga aggcgctcgc ctcacccgat
     1021 gtgaaggagc ggttcctcca gctcggggtc gacccgtgga cgatgaagcc ggccgacttc
     1081 gatgcctaca tcaagaccga ggtcgtctcc aatgcgaagc tggtgaagga cgctggcctg
     1141 acaccgacgc agtgagcgag cccgtcccct gctgacccga cggagtcgac catgcccacc
     1201 caacgtctcg gtctgatcat gcacggcgtc acggggcgca tgggcatgaa ccagcacctc
     1261 atccgctcca tcgtcgccat ccgcgaccaa ggcggtgtca cgctgtccaa cggcgacaag
     1321 gtgatgcccg acccgatcct catcggccgc aatgccgaga agatcgaggc gcttgccagg
     1381 ctgcatcgca tcgaacggtg gggcaccgat ctgaaggccg cactgggcga cgacagcgac
     1441 gcggtcttct tcgatgccgg caccacccag atgcgtccgt ccctgctgga agacgccctg
     1501 cgggccggca agcacgtgta ctgcgagaag ccgatcgcga ccgacctcga ccaggccgtg
     1561 aagatcgcga agatcgccca atcctcaggc ctcaagcatg gcgcggtgca ggacaagctc
     1621 ttcctgccgg gtctccgcaa gctcgacatg ctgcggcggg caggcttctt cggccgcatg
     1681 ctcagcgtgc ggctggagtt cggctactgg gtgttcgaag gcgacctgca gccgatccag
     1741 cggccgagtt ggaactaccg cgcagaggac ggcggcggga tgatcctcga catgatgt
//
LOCUS       DASVTM010000357         8037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_565379,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000357 DASVTM010000000
VERSION     DASVTM010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565379"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VF633_09680"
     CDS             <1..1156
                     /locus_tag="VF633_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX8471371.1"
                     /translation="GTECAVRPATDSLLESARGAAQAIAALEAPLLALARHLEDILDE
                     ESAELEPSSRARIEGALRGLDRRARMTLPGWRSMLAALQDGGDQPDPDFVDWLSVETA
                     FGRIHDVALRRHWIDPTIPLEAAVILPSHGVLVTSATLSDPIAAAAGGDPFDMARMRT
                     GSSRLIEPPRTLKVESPFDYEAHSRVIVVTDVAKDDPRQTAAAMRELFLAAGGGSLGL
                     FTAIRRLKAVYERLGPDLAKAQIPLYAQHVDPLEVGALVDIFRAEQDSCLLGTDAVRD
                     GVDVPGRSLRLLAFDRVPWPRPDLLHKARRLKFGGESAAGRSYDDSLARARIAQAFGR
                     LIRRADDKGVFVMLDAATPTRLFASLPPGAEVMRMGLVDAIELTRGFLAK"
     gene            1214..2269
                     /locus_tag="VF633_09685"
     CDS             1214..2269
                     /locus_tag="VF633_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEX8471372.1"
                     /translation="MSDIATKKHSRAGLFTPFILLLIVAVGWTGWWFYLADQVKTRFE
                     GQIDGLRTAGWDVRYTKLSMSGWPFRLRAQADRIAFDAPSGHGIAGPQLVAQASAWNP
                     DKWVLIAPDGLTVTRAGKGKVAVRGAGLRMSVSGLTQRWPNVVVELAQPEFTILPGAE
                     PFPISRAKLIQFYLRPHLAPAGTPGVETSVDMLFRLVDAEGRPNGPVEGMTRNGMLSA
                     QIETVVERADQLTGADAAGIFGAWTRAGGRFTDIQGELTAGDSRARLSSAVLSAQPDG
                     RLQGQLALKAEKPLPAIAGLAQSGTGAANQAGAMGAAAATAVAGGAGGNIDLTLVFRD
                     GRTFLGPFALAPAPKLF"
     gene            2276..2962
                     /locus_tag="VF633_09690"
     CDS             2276..2962
                     /locus_tag="VF633_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471373.1"
                     /translation="MLIEPEMDDATGALDRALAEAVKARLAAGGRPGPDAEALALRDL
                     LDHTDSVDQAALLRAVWGRISADSGPVLFAAGPTAHLLAADRHGFPVRSAADAEAALV
                     EAGRGARALIDIEGPRPWWGKLLARPDLRIVSALPDDRYGRPRSVMVSRETSGPTGED
                     RTFWITDSREPDVRIADALAGVGLLAQSLVSAGGLKLFMLAGYVQAEDGRLIDAPGFL
                     KGVIGAAPVF"
     gene            2988..4085
                     /gene="hisC"
                     /locus_tag="VF633_09695"
     CDS             2988..4085
                     /gene="hisC"
                     /locus_tag="VF633_09695"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX8471374.1"
                     /translation="MTDVTGPVPKPGILDIAPYVGGKSVIAGVAEPMKLSSNENALGA
                     GEKAREAYVAALKHIHIYPDGRASKLRTAVADYHQLEPERLIFGNGSDEVFALLNQTY
                     LTAGDNIVCGQYGFLAYQISAKACEATVKQAPEPNYKAEVDALLAEVDERTKIVYVSN
                     PSNPTGSYNTGEEIRRLHAALPAHILLVVDEAYAEFVTEPDWETALPLARDASNIIVT
                     RTFSKIHGLGGLRIGFGYAPLAVEQAIDRIRLPFNVSVAGLAAAEAAIADEAHQQASR
                     DLVETWRPRLTQSIKGFGFDVLPSAGNFVLVIFNGEKHTALAANDYLNTKGIIVRPVG
                     GYGLPNALRITVGTEDQNRAVIDALSEFAAS"
     gene            complement(4163..4876)
                     /locus_tag="VF633_09700"
     CDS             complement(4163..4876)
                     /locus_tag="VF633_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8471375.1"
                     /translation="MNFVRSVLFAGWLYLSMPVYAIGMSPALLMPHRYAMFVVQSWAR
                     GVLFGLRWIGGIEVEFRNLQYRPDGAALVASKHQSMLDIVALLAVLRDPCFVMKKELV
                     YMPFLGWFAWKTRMIAVDRAGHATALRDMTRQARERLLEHRQIIIFPEGTRNTPGAPG
                     EYKPGVAAIYRDLQASCALVATNSGEHWPAHGIRFVPGKVVFEFLEPAPAGMKRGPFM
                     ALLESRIEPASVALLETKG"
     gene            complement(4873..5541)
                     /locus_tag="VF633_09705"
     CDS             complement(4873..5541)
                     /locus_tag="VF633_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX8471376.1"
                     /translation="MKFLTLIGIVALMWLVGLFVFADRVRGLTPATEPARADAIVALT
                     GPSAERVNEAIRLLEHDKGDRVLISGVNPEVRRQELRALTPGSDRLFNCCVDLGFEAE
                     NTVGNAQEIGAWAKSHGYEHLIVVTSDYHMPRSLLEIRSVAPGIALTPYAIETPSLDN
                     SRWWRAAVTARRMTLEYMKYLAVLGRESLHRVTDKHPERGPASTVQARMESVPDKAAG
                     GARS"
     gene            complement(5603..6484)
                     /locus_tag="VF633_09710"
     CDS             complement(5603..6484)
                     /locus_tag="VF633_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8471377.1"
                     /translation="MKLAFLNRNPGLLPRDAAGETWLAAVIAVLCFMACLACVGAVAA
                     DRAAHGWARQLKAEATVQVRPRVGETGLAAAGRAAETLSSVPGVSEAAAMDRKTAEDL
                     LRPWLGEAVLPDLPLPFLVTVRLDQKAPASTIVLSRALAEAGLDASVDDHSLWRGEVE
                     RSAALITALSLAAFLLIACAAGAAIVYATRAGLQAQRSVIETLSLSGATEAYVASLFQ
                     TRFGLLAAGAGVIGSLAAAVLIAGLRALGGPGGLTAALPLAWSDILLLSPCPLLAATV
                     ALVAARIAAQTTLGGRH"
     gene            complement(6481..7191)
                     /locus_tag="VF633_09715"
     CDS             complement(6481..7191)
                     /locus_tag="VF633_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8471378.1"
                     /translation="MSAPHRRAAVSEPTPETVRLAGVGFGYADSPDVLRDVNLILPRG
                     SFHFLTGPSGAGKSSLLRLLTLAERPTSGRIGLFGQDVTDLGRADAPFLRRRMGVIFQ
                     DFRLLDHLDLFENAALPLRLVGRKRADYAPDVEEMLRWVGLGARMDARPPSLSGGEKQ
                     RLAIARAVMARPELIIADEPTGSVDKAMADKLLKLFQSLNKLGATVLIASHDEALAEQ
                     SGAQRLRLDRGRLTGGAA"
     gene            7263..>8037
                     /locus_tag="VF633_09720"
     CDS             7263..>8037
                     /locus_tag="VF633_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEX8471379.1"
                     /translation="MILTCPSCATSYFTPDEAIGPNGRKVRCKTCAHVWHASLNDEPL
                     ELDAPAAAAPNGIAAAAGKADAKTGFGKRDEVIESLAETPAPELPKAFRARAEEQRRV
                     RRAATHGAVWAGLASVFVAILAAGWLFRIEVVELYPRAAAAYAMIGSPVNPVGLQFEA
                     VGAKAMLNDPGKVVVSGALRNVREREVVAPPVRVALLDSHGAEIGHAVVTIDAAPVLP
                     GAVQGFAVVIADPGARGAGVGVDFVAVPKNTAVKAGRRAE"
BASE COUNT         1309 a         2730 c         2688 g         1310 t
ORIGIN      
        1 cgggaccgaa tgcgcggtcc gcccggcaac cgacagcctg ctggaaagcg cgcgcggcgc
       61 cgctcaggcg atcgcggcgc tcgaagcgcc gctgctggct ctcgcgcgcc atctcgagga
      121 catcctcgac gaggaatcag ccgagctcga gccgtcgtcc cgcgcccgga tcgaaggagc
      181 gttgcggggg ctcgaccgtc gcgcgcggat gacgctgccg ggctggcgct cgatgctggc
      241 ggcgctgcag gacggcggcg accagcccga tcccgacttc gtcgactggc tgtcggtcga
      301 gaccgccttc ggccgcatcc acgacgtggc cctgcgccgt cactggatcg atccgacgat
      361 cccgctcgag gccgccgtca tcctgccctc gcacggcgtt ctggtcacca gcgccaccct
      421 gtccgatcca atcgcagcgg cggcgggcgg cgatccgttc gacatggcgc ggatgcggac
      481 cggctcctcg cgcctgatcg agccgccccg caccctcaag gtcgagagcc cgttcgacta
      541 cgaagcgcac agccgggtca tcgtcgtcac cgacgtggcc aaggacgatc cgcgccagac
      601 ggcggcggcg atgcgcgaac tgttccttgc ggcgggcggc ggatcgctcg gcctgttcac
      661 cgccattcgt cgattgaagg ctgtgtacga gcggctgggt cccgatctgg ccaaggccca
      721 gattccgctc tacgcccagc acgtcgatcc gctggaggtc ggcgctctgg tcgatatctt
      781 ccgcgccgaa caggacagtt gcctgctcgg caccgacgcc gtgcgcgacg gtgtcgatgt
      841 gcccggtcgg tcgctgcgct tgctggcgtt cgaccgtgtg ccctggccgc gtccggacct
      901 gctgcacaag gcgcggcgtc tcaagttcgg cggcgagagc gcggcgggcc gcagctatga
      961 cgacagcctc gcccgtgcgc ggatcgctca ggcgttcggg cgtctgatcc gtcgcgccga
     1021 cgataagggc gtgttcgtca tgctcgacgc cgccaccccc acccgcctgt ttgccagcct
     1081 gccgccgggc gccgaagtca tgcgcatggg gctggtcgac gcgatcgaac tgacgcgcgg
     1141 ctttctggcc aagtaggcac ggcatgcgtc gtgccgcgtt catttcgcga cgcggctgct
     1201 gtaacaggac accatgagcg acatagccac caagaagcac agccgcgccg gactgttcac
     1261 gcccttcatc ctgctgctga tcgtggcggt ggggtggacc gggtggtggt tctatctggc
     1321 cgatcaggtg aagacccggt tcgaggggca gatcgacggt ctgcgcaccg caggctggga
     1381 cgttagatat accaagctga gcatgagcgg ctggccgttc cgtctgcggg cccaggccga
     1441 ccgtatcgcc ttcgatgcgc catcgggtca cgggatcgcc gggccgcaac tggtggctca
     1501 ggccagcgca tggaacccgg acaaatgggt gttgatcgcg ccggacggcc tgacggtcac
     1561 ccgtgcaggc aagggcaagg tcgccgtgcg cggcgcaggg ttgcggatga gcgtcagcgg
     1621 tctgactcag cgttggccta acgtcgtcgt cgagctggcc cagccggagt tcacgatcct
     1681 gccgggggcc gagccgttcc cgatctcgcg ggcgaagctg atccagtttt acctgcgtcc
     1741 gcatctggct ccggcgggta cgcccggcgt ggagaccagc gtcgatatgt tgttccgtct
     1801 ggtagacgcc gaggggcgtc ccaacgggcc ggtcgagggc atgacccgca acggcatgct
     1861 cagcgcccag atcgagacgg tggtcgagcg cgcagatcag ctcaccggcg ctgatgcggc
     1921 gggcatcttc ggagcctgga cccgcgcggg cggccgcttc accgacattc agggcgagct
     1981 cacggccggt gacagtcgcg ctcgcctgtc cagcgcggtc ctgtcggccc agcccgacgg
     2041 ccggctgcag gggcaactgg cgctgaaggc cgagaagccg ctgcccgcca ttgccggtct
     2101 ggcgcaatcg ggcacgggcg cggccaatca ggccggagct atgggcgccg ccgccgcaac
     2161 ggccgtcgcc ggcggtgcgg gtggcaacat cgacctgacg ctggtgttca gggacggccg
     2221 gaccttcctc ggtccgttcg cgctggcgcc tgcgccgaag ctgttctgac aaacgatgct
     2281 gatcgagccg gagatggacg acgcaaccgg ggcgctggac cgcgcgctcg ccgaggccgt
     2341 gaaggcgcgt ctggcggcgg gcgggcgacc cggtcccgat gccgaagcgc tcgccctgcg
     2401 cgatctgctg gatcacaccg acagcgtgga ccaggcggcc ctgctgcgcg cggtgtgggg
     2461 ccggatctcc gccgatagtg ggccggtcct gttcgcggcg gggccgacgg cgcatctgct
     2521 cgccgccgat cgtcatggtt ttcctgttcg gagcgccgca gatgctgagg ctgcgctggt
     2581 cgaggccgga cgcggcgctc gagccctgat tgacatcgaa ggtcctcgcc cgtggtgggg
     2641 caagctcctg gcccggcctg acctgcgcat cgtgtccgcc ctgccggatg atcgatatgg
     2701 ccggccgcgc tctgtgatgg tgtcgcgcga gacctcgggt ccgacgggcg aggaccggac
     2761 cttctggatc accgacagcc gcgagccgga tgtgcgcatc gccgacgctc tggctggtgt
     2821 cggcctgctc gcccagtcgc tcgtttcggc aggcggcttg aagctgttca tgctggcggg
     2881 ctatgtgcag gccgaggacg ggcggctgat cgatgcgccc ggtttcctta aaggcgtcat
     2941 cggcgctgca cctgtcttct gagcccactt tttccgagat tgcctgaatg accgacgtca
     3001 ccggacctgt gcccaagccc ggcatcctcg acatcgcccc ttatgtgggt ggcaagtcgg
     3061 tcatcgccgg cgtcgccgag ccgatgaagc tttcgtcgaa cgagaatgct ctgggcgccg
     3121 gcgagaaggc gcgcgaggcc tatgtcgccg cgctgaagca catccatatc tatcccgacg
     3181 gccgagcctc gaagctgcgc accgccgtcg ccgactatca ccagttggag cccgagcggc
     3241 tgatcttcgg caacggttcg gacgaggtct tcgccctgct gaaccagacc tatctgacgg
     3301 cgggcgataa tatcgtctgc ggccagtacg gcttcctcgc ctaccagatc agcgccaagg
     3361 cctgcgaggc cacggtcaag caggctcccg agcccaacta caaagccgag gtcgatgcct
     3421 tgttggccga ggtcgatgag cggacgaaga tcgtctacgt ctccaacccc tcgaacccga
     3481 ccggcagcta caacaccggc gaggaaattc gccgcctgca cgccgccttg cctgcgcaca
     3541 tcctgttggt cgtcgacgag gcctatgccg agttcgtcac cgagcccgac tgggaaaccg
     3601 ccctgccgct ggcccgggac gcgtcgaaca tcatcgtcac gcggacgttc tctaagatcc
     3661 acggcctcgg tggtctccgc atcggcttcg gctacgcgcc gctggcggta gaacaggcga
     3721 tcgaccgcat ccgcctgccg ttcaacgtct cggtcgccgg tctggccgcc gccgaagccg
     3781 ctatcgccga cgaggcccac cagcaagcct cacgcgatct ggtcgagaca tggcgtccgc
     3841 gcctgaccca gtcgatcaag ggcttcggct tcgacgtgct gccgtcggcc gggaactttg
     3901 ttctggtgat cttcaacggc gagaaacaca ccgccctcgc cgccaacgac tatctgaaca
     3961 ccaagggcat catcgtccgc cccgtcggtg gctacggctt gccaaacgcc ctgcgcatca
     4021 ccgtcggcac tgaggaccag aaccgcgccg tcatcgacgc cctcagcgag ttcgcggctt
     4081 cctagtaagg cgggcgctaa cgctcggctc gcggagcctc actgcttgag cgcaagaggg
     4141 aagcggggcg ttgccgctga cctcagccct ttgtttcgag cagggccaca gaggccggtt
     4201 cgatacggct ttctagcaac gccatgaagg ggccccgctt catgcctgcc ggagcgggct
     4261 cgaggaattc gaacaccacc ttgccgggga cgaacctgat cccgtgcgcg ggccaatgct
     4321 cgcctgagtt ggtggcgacg agtgcgcaac tggcctgcag gtcgcgatag atggcggcga
     4381 cccccggctt gtactcgccc ggagcgccgg gcgtgttgcg ggtcccctcg gggaagatga
     4441 tgatctgtcg atgttcgagc aggcgctcac gggcttgccg ggtcatgtcc ctgagcgccg
     4501 tcgcgtgccc tgcccggtct acggcgatca tcctggtctt ccaggcgaac catccgagga
     4561 agggcatgta gaccagctcc ttcttcatga cgaagcacgg gtctcgaagg acggccagca
     4621 gggccacgat gtcgagcatc gactgatgtt tggacgcgac cagagctgcg ccgtcgggcc
     4681 ggtactgaag gttccggaac tcgacctcga tgccgccgat ccagcgcagg ccgaacagca
     4741 cccccctggc ccaggactgc accacgaaca tggcgtaccg gtgcggcatc aacagcgcgg
     4801 gcgacatgcc gatcgcgtac accggcatcg atagatacag ccagccggcg aacaatacgg
     4861 aacgcacgaa attcaactgc gggctccccc ggcagccttg tccggaacac tttccatacg
     4921 ggcctggaca gtcgaggcgg gcccgcgctc cggatgttta tcggtcaccc ggtgcaagct
     4981 ttcgcgtcca aggaccgcga gatatttcat atattccagt gtcatgcgcc gcgccgtaac
     5041 cgccgcccgc caccagcgcg agttgtccag cgacggtgtc tcgatggcgt agggcgtgag
     5101 tgcgatgccc ggcgcgacgc ttcggatctc cagcagcgat cgaggcatgt ggtagtcaga
     5161 cgtcaccacg atcagatgtt catagccgtg actcttggcc caggcgccga tctcctgagc
     5221 gttgcccacg gtgttttccg cctcgaagcc gaggtcgacg cagcagttga acagccggtc
     5281 cgatccgggc gtcagggcgc gcaactcctg gcgccgcacc tcagggttga cgccggagat
     5341 cagcaccctg tcgcccttgt catgttccag cagccggata gcctcgttga cccgctcagc
     5401 cgaggggccg gtcagggcga cgatcgcgtc ggcgcgtgcg ggttcggtgg ccggcgtcag
     5461 gccgcggaca cgatccgcga aaacgaacag cccgaccagc cacatcagcg ctacgatgcc
     5521 gataagcgtc agaaacttca tctgaactcc ctatcgttgc taatggcgtc cgccaagagt
     5581 tcgcagacgg aatgtcccgt cttcaatgtc gcccacccaa tgtggtctga gccgcaattc
     5641 gcgccgcgac aagcgctacc gtagcggcca atagcggaca tggcgagagc agcaggatat
     5701 cgctccacgc cagaggcagg gcggcggtca gcccccccgg gccacccaga gctctgagac
     5761 ccgcgatcag cacggctgca gcaagcgacc cgatcacccc ggcgcccgcc gccagtaggc
     5821 cgaaccgcgt ctggaacaga ctggcgacat aggcttctgt ggctcctgag aggctcaggg
     5881 tctcgatgac gctacgctgg gcctgcagtc cagcccgggt ggcgtagact atggcagccc
     5941 cggccgcaca ggcgatcagc aggaaggcgg ccaggctcaa cgcggtgatc agtgcggccg
     6001 agcgctcgac ctcgccgcgc cacaggctgt gatcgtcgac gcttgcgtcc agaccggcct
     6061 cggccagcgc ccggcttagc acgatggtgc tggcgggggc cttttgatcc agacggacag
     6121 tgacgaggaa aggcaggggc agatcgggca ggactgcctc gccaagccag ggtcgcagca
     6181 gatcctctgc tgtcttgcgg tccatggctg cggcctcgga cacgccggga acactcgaca
     6241 gcgtttcggc ggcgcgtccc gcggcggcca gaccggtttc cccgacacga ggtcgcacct
     6301 gaaccgtcgc ctcagccttg agttggcggg cccagccatg tgcggcccgg tcggcagcaa
     6361 cggctccgac gcaggccagg caggccatga aacagagcac ggcgatcaca gccgccagcc
     6421 aggtctcgcc tgctgcgtca cggggcagca ggccgggatt gcgattgagg aaggcgagct
     6481 tcacgcggcg cctccggtca gtctgccccg gtcgagccgc aagcgttgcg cgcccgactg
     6541 ttcggccagc gcctcgtcat gactggcgat caggacggtg gcgcccagct tgttcagcga
     6601 ctggaacagc ttcagcagct tgtccgccat agccttgtcg acgctgccgg tgggctcgtc
     6661 ggcgatgatc aactccggtc gggccatcac cgcccgggcg atggccagtc tctgtttctc
     6721 gccgcctgac agcgacggtg gtctcgcgtc catgcgtgcg ccgagcccga cccagcgcag
     6781 catctcctcg acgtcgggcg catagtcggc ccgctttcgc ccgaccagcc tcagcggcag
     6841 ggcggcgttt tcgaacaggt cgaggtgatc cagcagcctg aaatcctgga aaatcacgcc
     6901 cattctccgc cgcagaaaag gggcgtcggc ccggcccagg tcggtcacat cctgtccgaa
     6961 cagcccgatc cgaccggagg tcgggcgttc agccagcgtc aaaagcctga gaagtgacga
     7021 ctttccggct cccgaaggcc cggtaaggaa gtggaaagag ccacgcggca gaatgaggtt
     7081 aacgtcccga agcacgtcgg gcgaatcggc atagccgaaa cccacgcccg cgaggcggac
     7141 ggtttcgggc gtgggttcag aaacggcggc gcggcggtgc ggagcagaca tggattttct
     7201 ttgggaccgg ggcgcgcgcg cctcgacagg agggggcgga tgacgccccg cagtcagacc
     7261 acatgatact gacctgcccg tcctgcgcca ccagctattt cacgcccgat gaggcgatcg
     7321 gtcccaatgg acgcaaggtc cggtgcaaga cctgtgcgca tgtctggcat gcgtccctga
     7381 acgacgaacc gctcgagctc gacgcacccg ctgcggccgc gccgaacggg atcgccgccg
     7441 cagctggcaa ggctgatgca aagaccggtt tcggcaagcg cgatgaggtg atcgaaagtc
     7501 ttgccgaaac ccccgctcct gaactgccca aggcctttcg cgcccgggcc gaggaacagc
     7561 gccgcgtccg ccgcgccgcc actcacggcg ccgtctgggc tgggctggcc agcgtcttcg
     7621 tcgcaatcct cgccgccggc tggctgttcc ggatcgaggt ggtcgaactc tatcctcgcg
     7681 ccgccgccgc ttacgctatg attggctcgc ccgtgaaccc ggtaggcctg cagttcgagg
     7741 ccgtcggcgc aaaggccatg ctcaacgatc cgggcaaggt cgtggtctcc ggcgctctcc
     7801 gaaacgtacg tgaacgcgaa gtggtggccc cgcccgtccg ggtcgccctg ctggacagcc
     7861 acggcgccga gattggtcat gcagtggtga ccatcgacgc cgctccggtt ctgcccggcg
     7921 ccgtccaggg cttcgcggtc gtcatcgccg atcccggggc cagaggcgcg ggcgtcggcg
     7981 tcgatttcgt tgctgtgccg aagaacacag cggtcaaggc tggacgcagg gcggagg
//
LOCUS       DASVTM010000358         8162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_351715,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000358 DASVTM010000000
VERSION     DASVTM010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351715"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VF633_09725"
     CDS             <1..274
                     /locus_tag="VF633_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471380.1"
                     /translation="VILMDIQMPVMDGVAATRAIRALGDYRAGCPIVAVTANTLAAQL
                     DSYAAAGMNDWIEKPVNINILLDKTTGWAACGWRESVAEPAAKAAI"
     gene            379..798
                     /locus_tag="VF633_09730"
     CDS             379..798
                     /locus_tag="VF633_09730"
                     /inference="COORDINATES: protein motif:HMM:NF018232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEX8471381.1"
                     /translation="MSCCASLALGFLPWAMFVGWVAWYTRPTSFRQGVATWLCLSGGL
                     VFGALAVMALGVLSPSMGIFALSAVVFFVALTVVSMRNVRLLDNIPAWFLGLIAFFAS
                     HMEPGVISVAELGATSAMGVDGGWLSVQLQRRLATTH"
     gene            complement(1099..2001)
                     /locus_tag="VF633_09735"
     CDS             complement(1099..2001)
                     /locus_tag="VF633_09735"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8471382.1"
                     /translation="MDRETWNDLAVFSAIVEAGGFTRAGVGLGVSASALSHRMRLMEA
                     RLGLRLLNRTTRSIAPTEAGERLLMSLRPAIAEVDAALSALDGDRVRPAGRVRVSAHR
                     SAAFGLVLPRLSRFADAYPEITVELVVDDGLVDIVSAGFDAGIRRLPSLEQDMVSVRL
                     DDGVRLTIAASPVYLETFGTPQAPADLQRHRCLNYRLPTAGSLYRWTFERDGQTTTMD
                     VPGGFVTNDIDMLCEGAVAGLGLACVIETQAAPYLSSGTLVSVLEDWRPMLPPNYLYY
                     AGRRNPPPALRVFIDAMKETIAGN"
     gene            2098..3195
                     /locus_tag="VF633_09740"
     CDS             2098..3195
                     /locus_tag="VF633_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8471383.1"
                     /translation="MSLDRYRPLGRSGLIVSPLALGTMTFGAARWGMERADAEAVFDA
                     YAEAGGNLIDTADVYASGGGEAMLGEIITERGNRDRLVLATKSGFAAGQGPSAGGNGA
                     KHIHAALEGSLRRLKTDYIDLYWIHVWDSVTPPEELLETMSALVRSGKIRYWGMSNTP
                     AWYVARIVTLAAASGRPGPIAMQYFYSLVSREIEAEHVPLALDTGLSIMPWSPLAYGL
                     LTGKYDRATVEAAPSRAGGLPNEAANGGAESGARLDGANPFGDSLFTNRNWAIVDALK
                     AIAAKIGEPPARVALAWALSRPAVDTVLVGVSRVSQLQDNMSAIDLRLSAEDMAALQA
                     ASRPDLPMLYGLFSDEMRRHVVYGGAAVSGR"
     gene            complement(3258..3542)
                     /locus_tag="VF633_09745"
     CDS             complement(3258..3542)
                     /locus_tag="VF633_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471384.1"
                     /translation="MADVVPQIVWITDAEGRVEFLNRQWSDYRVSGATPTTATEVAAL
                     FLPQAPASGLEVDLPLIASFQRNRSRTPQYCPISRSKPPPSTLRPGVIPR"
     gene            complement(3680..4198)
                     /locus_tag="VF633_09750"
     CDS             complement(3680..4198)
                     /locus_tag="VF633_09750"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HEX8471385.1"
                     /translation="MTDLSRRGVLGAALAGTGAVLPPASAIAQTRHAAPAPLPPPADP
                     TPRAVVYFAPERFDDLMWENDRTAHRIYGHALQANEPPSGSGIDVWGKKVRWPYVNET
                     RGYGNLITDPAFQTAQKRQGRTLMLGRKSDIRSDHCEQPDDMRCPTKLGLAELFLTAP
                     PFVFRSADRNPR"
     gene            complement(4195..6657)
                     /locus_tag="VF633_09755"
     CDS             complement(4195..6657)
                     /locus_tag="VF633_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8471386.1"
                     /translation="MAAALAALLAGQATPGLGQSIGDATAHPALWPTAHSPAALTGPA
                     TEARVRELLSRMTIEEKVGQLIQADIGAITPADLAAYPIGSILAGGSSSPNGDERSPP
                     QAWLELSRAYRAAALARPGAAIPLLFGVDAVHGHNNIVGATLFPHNIGLGATRDPELI
                     RRIVEATAAEVAATGANWTFGPTLAVPRDDRWGRTYEGFAETPQVARSYAGAFVEGLQ
                     GPLRDGGPLASGHVMGSAKHFLADGGTADGRDRGDAVVSEQELIDIHLSGYREAIDAG
                     VLSIMVSFSGWNGVKHTGNRSLLTDVLRGPLGFDGLLIGDWDAHAFVPGCRPDSCPAA
                     INAGLDMFMAPNAWKPLFVNTLAQARSGEISAARLDEAVRRILRVKVVLGVLDAPNPA
                     EGDVAVIGSADHRALARRAVGESLVLLKNNDATLPIRAGARVLVVGDAADDIGQASGG
                     WTISWQGTGNTNADFPGATSIWAGVRDVIMAGGGSASLSPDGAFTDRPDVAIVVFGET
                     PYAETYGDLDTLDFAPTGPLETLKRLKAAGVPTVSVFLSGRPLWTNPEINASDAFVAA
                     WLPGSEGAGIADVLIGNAEGKARHDFKGVLGFSWPRSAGQSPQNVGQPDYDPQFAYGY
                     GLRYGAPAQTPILSEEPGVSPDAAGPPRFFANGRFVAPWALTLRDSSGNFRVEAPGPA
                     SSPGGALTLAPVDGQSQESARRLTFVGGREASAVIGGAPTRFAPGLALRLSYRLESPP
                     AEDTTLWLGSRGLRIASALKTETGWTSLTVPLSCFGGAADATVSAIRLGTAGAMTLSF
                     EEVRLTSAGDASCACFSEAPTA"
     gene            complement(6675..7898)
                     /gene="manD"
                     /locus_tag="VF633_09760"
     CDS             complement(6675..7898)
                     /gene="manD"
                     /locus_tag="VF633_09760"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEX8471387.1"
                     /translation="MARKTAQIVDARIVVTCPGRNFVTLKITTSDGTYGLGDATLNGR
                     EMAVAAYLNDHILPLLIGKDAHRIEDMWQYLYRGAYWRGGPVGMTAIAAVDVALWDIK
                     AKIAGLPLYQLLGGACREGVTVYGHANGSTIEETVQEALKYRALGYRAIRLQSGVPGL
                     NSTYGVSRDKMFYEPADAALPTENEWSTPRYLRMVPELFKAAREALGWDVELLHDVHH
                     RLTPIEAGRLGNDLEPYRPFWLEDAVPAENQAAFRTIRAHTTTPLAVGEIFSSIWDCK
                     TLIEEGLIDYIRATVVHAGGITHLRRIAALADLHHVKTGCHGATDLSPVSMAAALHFG
                     LSVPNFGIQEYMRHTPETDAVFPHEYGFEDGMLHPGDAPGLGVDLDETLAATHPYSRA
                     YLPVNRLEDGSMFHW"
     gene            8012..>8162
                     /locus_tag="VF633_09765"
     CDS             8012..>8162
                     /locus_tag="VF633_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8471388.1"
                     /translation="MTTSTADTRKLYQQVAGSVSAGIAEGRYPVGTRLPSERDLAEEF
                     GVSRPT"
BASE COUNT         1381 a         2574 c         2838 g         1369 t
ORIGIN      
        1 tgtcatcctc atggatatcc agatgcccgt catggacggc gtggcggcaa cacgcgctat
       61 ccgggctttg ggggactacc gcgccggctg cccgatcgtg gccgtgacgg ccaacactct
      121 ggccgctcag ttggatagct atgctgcagc gggcatgaat gactggattg aaaagccggt
      181 aaacatcaac attctgcttg acaagacgac agggtgggcc gcttgcggct ggcgcgagag
      241 tgtcgccgaa cctgcagcca aagccgcaat ctagatcctg gccatgatgg ccagcccgaa
      301 aggcagcgag gcgccccctt ggagggtgac tgccgttcct tcaatgcaag gcgggaaggg
      361 agggaactga gccatcaaat gagttgttgc gccagtctcg ccctcggttt cctcccctgg
      421 gccatgtttg tgggctgggt cgcttggtac acccgcccca cttcgtttcg tcagggtgtt
      481 gcaacctggc tgtgcttgtc tggcggtctc gtctttgggg cgctcgctgt catggcgctc
      541 ggcgttctct cgcccagcat gggtatcttc gccctcagcg cggtcgtctt cttcgtcgcc
      601 ctcaccgttg tttcgatgcg caacgtccgc ctcctggaca acatccccgc atggttcctc
      661 ggactgatcg ccttcttcgc ctcacacatg gagccgggcg tgatctcggt ggccgaactg
      721 ggagcgacgt cggcgatggg cgttgacggc ggctggctgt cggtgcagct gcagcggcgt
      781 ctcgcaacga cgcattgagt aagtgagggg gacggggcgg actttgtccg tagccaggat
      841 gcggctcaag gtcagtctac gccagaagct cggagcttta aggtcgtggt ggatgaccct
      901 cgctgtcgga acttgtcaga ttccgccttc agtccgacgt caatgtttgc tgtaggcgct
      961 tggctgccat tggaacctct gcttccggga aggttcgtca ggcgttgcag ggcaggaacg
     1021 taatgtccgt ttctgggatg gcggggggta atcgaagtcc gctttgtgag agcgccaggc
     1081 ggcgttggcg gcctcgtttc agtttccggc aatggtctct ttcatcgcgt cgatgaagac
     1141 gcgaagagcc ggaggcggat ttcgacggcc agcgtaatag aggtagttgg gcggcagcat
     1201 cggacgccag tcctcgagga cgctcaccag ggtgccggag gaaaggtagg gcgccgcctg
     1261 ggtctcgatc acgcaggcca agccaaggcc ggccacagcg ccctcgcaca gcatgtctat
     1321 gtcgttggtt acgaaaccgc cggggacatc catcgtcgtc gtctgaccgt cccgctcgaa
     1381 tgtccatcga tagagcgatc ctgccgtcgg caggcggtag ttgagacaac gatggcgctg
     1441 cagatcggcg ggcgcctgcg gcgtgccaaa ggtctccaga tagacgggcg aggcggcgat
     1501 ggtcaggcgc acgccgtcgt ccagccggac cgagaccatg tcctgctcca gcgacggcaa
     1561 ccggcgtatg ccggcgtcga agccggcgct gacgatgtcg accaagccgt catccacgac
     1621 cagttcgacg gtgatttcag ggtaggcgtc agcaaaccgg gacagacgcg ggagcaccag
     1681 accgaaggcc gccgaccggt gggcggagac ccggacgcga cccgccggcc ggacccggtc
     1741 tccgtccagg gcgctgaggg ccgcatcgac ctcggctatc gccggacgaa gactcatcaa
     1801 caagcgttcc ccagcctcgg tcggagcgat gctgcgcgtc gtgcggttga ggagacgaag
     1861 ccccagccga gcctccataa gacgcattcg atggctcaag gccgaggccg agacgcccag
     1921 gccgacgccc gcgcgcgtga agccgccggc ttcaacgatg gccgagaaga cggccaggtc
     1981 gttccaggtc tcacgatcca ttgttgagct gacctcatgg gggtttgcaa tcaactgccg
     2041 ctaccgctca accctcgaca tgtccattct aagccccgga acagacagga gcgtcccatg
     2101 tccctcgacc gctatcgccc gctcggccgt tcaggcctga tcgtcagccc gctcgcactt
     2161 ggcacgatga cgttcggcgc tgcgcgctgg ggaatggagc gagccgacgc cgaagccgtg
     2221 ttcgacgcct atgctgaggc aggcggcaat ctgatcgata ccgcggacgt ctacgcgagc
     2281 ggggggggcg aggcgatgct gggcgagatc atcactgagc gcggaaaccg cgacaggctg
     2341 gtgctggcga ccaagtcggg cttcgcggcc gggcagggtc caagcgccgg cggcaatggc
     2401 gccaaacaca ttcacgctgc gctcgagggt tcgctgcgcc ggctcaagac cgactacatc
     2461 gacttgtact ggatccacgt ctgggactcg gtgactccgc cagaggaact gctggagact
     2521 atgtccgcac tcgtgaggtc gggtaaaatc cgctactggg gcatgtccaa caccccggcc
     2581 tggtacgtgg cccggatcgt gaccctggcc gcggcgagcg gaaggccggg tcctatcgcc
     2641 atgcaatatt tctattctct ggtcagccgc gagatcgagg cggagcatgt gccgctcgca
     2701 ctggatacgg gtctcagcat catgccgtgg agcccgctcg cttacggcct gctgaccgga
     2761 aagtatgatc gagcgactgt ggaggccgcg ccgtcgcgcg ccggggggct gcccaatgag
     2821 gcggcgaacg gcggcgcgga aagcggagca aggctcgacg gcgccaatcc tttcggcgac
     2881 agcctgttca ccaatcgcaa ctgggctatc gtcgacgctc tgaaggccat cgccgccaag
     2941 atcggcgagc cgcccgcccg cgtcgctctc gcctgggccc tctccaggcc cgccgtggat
     3001 acggttctcg tcggcgtgag ccgcgtgagc caattacagg acaacatgag cgcgatcgat
     3061 ctccggcttt cggcagagga tatggccgcg ctgcaggctg cgagccggcc agacctcccc
     3121 atgctctacg gtctgttcag cgacgagatg cggcggcacg tcgtctatgg cggcgccgcc
     3181 gtttccgggc gttaggaggc agatcctccg cccgttgtgc gccggtcagt cgcgcatgat
     3241 tgcggcgaca gccgctttca tctcgggatc acgcccggtc gcaatgtcga cggcggtggt
     3301 ttggatctgc tgatcgggca gtactgcggc gtccggctgc ggtttctctg gaaactggcg
     3361 atcaacggca ggtccacctc caggcccgac gcgggagctt gaggaagaaa aagggcggcg
     3421 acctccgtgg cggtggtggg ggtggcgccc gacaccctgt agtcgctcca ctgcctgttt
     3481 aagaactcga cgcgaccctc tgcgtccgtg atccagacaa tctgggggac cacgtcggcc
     3541 atcagtcgga accgggcctc attctccagc agccgcctgc agttccctag gtccggtctc
     3601 ttcgcgcatc gtcgtttcgg tccggagcca gggttcggtt cacgggcgac tgctctggac
     3661 gcccgtgagg gttaagcttc taccgcggat tccgatccgc cgagcgaaac acaaacggcg
     3721 gcgctgtcag aaatagctca gctaatccga gcttggttgg gcaacgcata tcgtcgggct
     3781 gctcacagtg atcggatcga atgtcgcttt tccggccgag catcaatgtc cgtccctggc
     3841 gcttctgagc cgtctggaac gccggatcag tgatcaggtt gccgtagccc cgggtctcgt
     3901 tgacataggg ccagcgcacc ttcttgcccc agacatcgat gccagaaccc gacggcggct
     3961 cgttagcctg aagcgcgtgg ccgtagattc ggtgagcggt gcgatcgttc tcccacatca
     4021 agtcatcgaa ccgctcaggc gcgaaataga cgacagcgcg gggcgtggga tcggcgggcg
     4081 gcggcagggg cgcgggcgcg gcgtgacggg tctgggcgat ggcggaggcg ggcggcagca
     4141 cggcgccggt tccggcgagg gcggcgccga ggacgccgcg tcggctaagg tcggtcatgc
     4201 ggtgggagcc tcgctgaagc aggcgcagga agcgtcgccg gcggacgtca ggcggacctc
     4261 ctcgaacgac agggtcatgg ccccggccgt gccgaggcgg atcgcagaaa ctgtcgcatc
     4321 cgccgcgccg ccaaagcagg acaggggcac ggtcaggctt gtccagccgg tctcggtctt
     4381 gagcgcggag gcgatccgca ggccgcgcga accaagccac aaagtcgtat cctcggccgg
     4441 ggggctctcc agtcggtagc tcaggcgcag cgccaggccg ggcgcaaacc gggtcggtgc
     4501 gccgccgatc acggcggagg cctcgcgacc gcccacgaag gtgaggcgac gagcggattc
     4561 ctgggactgg ccgtcgacgg gggcgagggt cagagcgccg ccggggctgg aggccgggcc
     4621 gggcgcctcg acgcgaaagt ttccagacga gtcgcgtaga gtcagggccc agggagcgac
     4681 gaaccggccg ttggcgaaga agcggggcgg tccggcagcg tcaggcgaga cgccgggctc
     4741 ttcggacagg ataggggttt gggcgggcgc gccgtagcgc aagccgtagc cataggcgaa
     4801 ctgcggatcg tagtcgggct ggcccacatt ctgcggcgac tgtccggcgc tgcggggcca
     4861 tgagaagccg agcacgcctt tgaaatcgtg gcgggccttg ccctctgcat tgccgatcag
     4921 gacgtcggcg atccccgcac cctcgctccc gggcagccag gcggcgacga aggcgtcgga
     4981 tgcgttgatt tccgggttgg tccacagcgg gcggccggac aggaagacgg agaccgtggg
     5041 gacgccggcc gccttcagac gcttgagagt ctcgagcggg ccggtcgggg cgaagtcgag
     5101 ggtgtcgaga tcgccgtagg tctcggcgta gggcgtctcg ccgaaaacga cgatggcgac
     5161 atcgggcctg tccgtgaagg cgccgtctgg gctaaggctc gctgatccgc cgcccgccat
     5221 aatcacatcg cgaacgccgg cccagatcga ggtcgcacct gggaagtcgg cattggtgtt
     5281 gccagtcccc tgccagctaa tcgtccagcc gcctgaggcc tgaccgatgt catcagcggc
     5341 gtcgcccacc accagaaccc gggctccggc gcggataggc agggttgcgt cgttgttctt
     5401 gagcagcacg agggactcgc cgacagcccg tctcgccaag gcgcggtgat ccgccgaccc
     5461 gatgaccgcg acgtcgccct cggccgggtt gggagcatcg aggacgccca gaaccacctt
     5521 gacgcggagg atgcgtctga cggcttcgtc cagacgggcg gcgctgatct cgcccgaccg
     5581 ggcctgggcc agggtgttca cgaagagtgg cttccaggcg ttgggggcca tgaacatgtc
     5641 gagcccggcg ttgatcgcag ccgggcaact gtcgggccgg cagcccggga cgaaggcgtg
     5701 agcgtcccag tcgccgatga gcagaccgtc gaagccgagc gggccgcgga ggacgtcggt
     5761 cagcagcgag cggttgcccg tgtgcttgac cccattccag ccggagaatg acaccatgat
     5821 cgacagaaca ccggcgtcga tggcctctcg atagccggac aggtggatat cgatcagctc
     5881 ttgttccgac accacggcgt cgccccggtc gcggccatcg gcggtgccgc cgtcagcgag
     5941 gaaatgcttg gcggacccca tgacatggcc ggaggcgagg gggccgccat cgcgaagcgg
     6001 gccctgaagg ccttcgacga aggcgcctgc atagctgcgg gccacttggg gggtctcggc
     6061 gaagccttca taggtgcgac cccatcggtc gtcgcgcggc actgccaaag tcgggccgaa
     6121 ggtccagttc gcgcccgtgg cggcgacctc ggccgccgtg gcctcgacga tgcggcggat
     6181 aagctcggga tcccgagtgg cgccgagccc gatgttgtgc ggaaacagtg tcgcgccgac
     6241 gatgttgtta tgaccgtgga cggcgtccac cccgaacagc agggggatgg cggcgcccgg
     6301 ccgggccagc gctgcggcgc ggtaggcgcg tgacagctca agccaggcct gcgggggtga
     6361 tcgctcgtcg ccgttgggcg atgagctccc gcccgccaga atcgagccta tcgggtaggc
     6421 cgccagatca gccggtgtga tcgcgccgat atcggcctgg atcaactggc cgaccttctc
     6481 ctcgatcgtc atgcgcgaga ggagctcgcg cacccgggct tcagtcgcgg gccccgtcag
     6541 ggcggcggga ctgtgagcgg tcggccaaag cgccggatgg gccgtggcgt cgccgatgga
     6601 ctggcccaag cccggcgtcg cctgccccgc cagaagggcc gcgagagcag ccgccaagag
     6661 gtgtctgcct gggtctacca atggaacatg ctcccgtcct caagtcggtt caccggcaaa
     6721 taggcgcggc tgtagggatg cgtggccgcc agcgtctcgt cgaggtctac gcccagcccc
     6781 ggggcgtccc ccgggtgaag catgccgtcc tcgaagccat attcatgcgg gaagaccgcg
     6841 tcggtctcgg gcgtatgacg catgtattcc tgaatgccga aattcgggac ggacaggccg
     6901 aaatgcaggg ccgccgccat cgagacgggc gacaggtcgg tggcgccgtg acagccggtc
     6961 ttgacgtggt gcaggtcggc gagggcggcg atccggcgca gatgggtgat ccctccggcg
     7021 tggaccacgg tggcgcggat atagtcgatc agcccttcct cgatcagggt cttgcagtcc
     7081 cagatcgagg agaagatctc gccgacggcc agcggcgtcg tggtgtgggc gcggatggtg
     7141 cggaaggcgg cctggttctc ggcgggcacc gcatcctcca gccagaaggg gcggtacggc
     7201 tcgaggtcgt tgccgagacg tccggcttcg attggcgtca gccggtggtg cacgtcgtgg
     7261 agcagttcga catcccagcc aagcgcctcg cgcgccgcct tgaacaactc cggcaccatg
     7321 cggaggtagc gcggggtcga ccattcattc tcggtcggca gggccgcatc ggcgggttca
     7381 tagaacatct tgtcgcgtga cacgccgtag gtgctgttca ggccgggcac cccggactgc
     7441 agccggatgg cgcggtagcc gagcgcgcgg tatttcagcg cctcctgcac cgtctcctcg
     7501 atcgtcgagc cgttggcatg gccataaacg gtgacgccct cgcggcacgc tccgcccagc
     7561 aactggtaca gcggcagacc cgcgatcttg gccttgatgt cccacagagc cacgtcgacg
     7621 gcagcgatgg ccgtcatccc caccggccct ccacgccagt aggcgccccg gtagaggtat
     7681 tgccacatgt cctcgatacg atgtgcgtcc ttgccaatca gcagcggcag aatgtgatcg
     7741 ttgagatagg cggcgaccgc catctcacgc ccgttcaggg tggcgtcgcc caggccgtag
     7801 gtcccatccg acgtggtgat cttcaaggtt acgaaattgc gcccggggca cgtcaccaca
     7861 atgcgagcat ccacgatctg ggccgttttt cgcgccatgc gcgacgattt ggatgaggcg
     7921 atctgggtct gggtcatcgg catgttactc aagaaaacga aagcacgggc ggactggcct
     7981 gacccgcgtg ggcgacgtag aaggatgcgg catgaccaca tccaccgccg acacccgaaa
     8041 actgtatcaa caagtcgccg gatcggtctc ggccggcatc gccgagggcc gctatccggt
     8101 cgggacgcga ctgccttcgg aacgcgatct cgccgaggag ttcggcgtga gccggccgac
     8161 gg
//
LOCUS       DASVTM010000359         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_883368,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000359 DASVTM010000000
VERSION     DASVTM010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883368"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..762
                     /locus_tag="VF633_09770"
     CDS             13..762
                     /locus_tag="VF633_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8471389.1"
                     /translation="MSVYSGRFAGRCAVVTGGASGLGKATAARIVAEGGKVVLWDLNA
                     DALKAAAEEIGALDTIALDVSDADAVAAAAEASNAILGKIDILVASAGITGATVPVHE
                     FPIDSWKRVMDINLNGVFYCSRAVVPFMLANGYGRIVNVASVAGKEGNPNASAYSASK
                     AGVIGFTKSLGKELAGKGVIANALTPATFESPILAQMPQSQVDYMRSRIPMGRLGEVD
                     ESAAMVCFMASEECSFTTASTFDTSGGRTTY"
     gene            773..1675
                     /locus_tag="VF633_09775"
     CDS             773..1675
                     /locus_tag="VF633_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8471390.1"
                     /translation="MASDLRIVDPHVHLWDLSRARYSWLQDDPLPNNPAGDMSGIAGR
                     NYGLSDYLADTSGWQVDKIVHVECGLPRGEQLAETDWLQAMAEAYGYPQGIVAGANML
                     DPDLDALLEAHAARPNVRGVRQIVCWHEDPSKTYTDRDLLLDPAWEVGFGKLAPHGLS
                     FDLQLYPSQMATAFVIAARHPDVPMIVNHAGLPTDRDADGMKRWRTGLRLLAAQPQVS
                     IKISGLGITDRAWTAETLRPIVLECIDAFGTERAMFASDFPVERTHGSFEAFFSAFDA
                     ITADFSVTERERLFAANAEAIYRI"
     gene            1742..3004
                     /locus_tag="VF633_09780"
     CDS             1742..3004
                     /locus_tag="VF633_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEX8471391.1"
                     /translation="MSIEIHAAHTPGGLAAPAGSLKGPLAFAIACFLIWGLAYGLLDV
                     LNKHFQETLSISQADSSWLQIAYFGAYLLLSVPAGMLLHAKGYKFGIVTGLAVTAIGA
                     LLFIPAAGAGAFMPFVGSMFVLAAGLCILETSADTYVNVLGDPAKASQRLNLAQSFNA
                     LGVFFGPLIGGAVFFSPTTTEALGGATRSIQVVYGVIAVGVVIFAFAVWRARLPETGL
                     SDHGDAVAGDAPARPLSQQPHFIAGVITQALYIGAQVGIGAYFINLVTHNWNGLTSQQ
                     GAFMLSLAAIGYLVGRFAATALLLRVKPRVLLTTYGLINVALTLIVVAGIERVSPIAL
                     IAVFFFMSTMFATIFALGTTGLGAGTKKASSLMVMAIGGGVLLPWPMGKIADLYGANI
                     AFILPCVCFAVVAWYGWKGAVLGRTERV"
     gene            3004..>4783
                     /locus_tag="VF633_09785"
     CDS             3004..>4783
                     /locus_tag="VF633_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471392.1"
                     /translation="MRIRSALLAFTLLTAPAAAMAQTTPAAIVAPAHPIDRQALVSRH
                     NVVLTAIDRHAPIMLGNGALGFTADITGLQTFPEQYSELAPLMTMAQWAWHSFPNPEG
                     YTEQNGLVQVPVPGRGDQPYAWMKSWADADTNPAYNWLRANPHRFSLARIGLILKDGQ
                     ALDFAKVSGATQTLDLWTGALTSRFTYDGQPVEVVTRVHPTLDMVMVEIASPLVASEG
                     LGVQVRYPEVNPAINPDTTLFEGDGAQSLSVVAHDTGDIRIRRTLDETTYYSSITASG
                     KVDQTGADAFRVVSTGRDRLTVMVRFDQQAEAPATPAYSAGVQAATAQWHDFWTKGGA
                     VDFSGSTDPRAAELERRVVLSQYLSAVNGAGALPPQEEGLFSNSWYGKFHLEMHPWHS
                     GWQALWGHPEMLERSLPWYVGNLANARAEAARHHVQGAWWPKMVGPDGRNSPSLVSPF
                     IMWQQPHSILLAELVWRAEKDPAVLGRYGEVVEATADLLASWPIERDGRLNLGTPMIP
                     AQENYDPLTTTNPTFELEYFRWALETAQAWRERKGQPRKAEWDRALAKLASPPMKDGL
                     YLPIENAPDFWTIAASDACRRHASEPQ"
BASE COUNT          770 a         1638 c         1511 g          864 t
ORIGIN      
        1 agcgtggtcg cgatgagcgt ctattcaggg cgtttcgccg gccgctgcgc cgtcgtcacc
       61 ggcggcgcct ccggtctggg caaagccacc gccgcccgca tcgtcgccga gggcggcaag
      121 gtcgtcctct gggacctgaa cgccgacgcg ctcaaggccg cggctgagga gatcggcgcg
      181 ctcgatacga tcgctctcga tgtttccgac gccgatgccg tggccgccgc tgccgaagcc
      241 tcgaacgcga tcctcggcaa gatcgacatc ctcgtcgcct cagcgggcat cacgggcgct
      301 acagtgccgg tgcacgagtt tccgatcgac agctggaaac gggtgatgga catcaacctg
      361 aacggtgtct tctactgctc gcgcgccgtc gtgcccttca tgctggccaa cggctacggc
      421 cggatcgtca acgtcgcctc ggtggcaggc aaggaaggca acccgaacgc ctcggcctat
      481 tcggcgtcga aggccggcgt gatcggcttc accaagtcgc tgggcaagga actggccggc
      541 aagggcgtga tcgccaacgc tctgacgccc gccacgttcg agagcccgat cctggcccag
      601 atgccccaaa gccaggtcga ctatatgcgc tccaggatcc cgatgggccg tctgggcgaa
      661 gtcgacgaaa gcgccgccat ggtgtgcttc atggcgtccg aagaatgcag tttcaccacc
      721 gcctcgacgt tcgacacgtc gggcggacgc acgacctact agggccgcgc gggtggcgtc
      781 ggaccttcgc atcgtcgatc cgcacgtcca cctgtgggac ctgtcgcgcg cccgctatag
      841 ctggctgcag gacgaccccc tgcccaacaa tccggcaggg gacatgagcg gcatcgccgg
      901 tcggaactat ggcttatccg actatctggc cgacacatcc ggttggcagg tggacaagat
      961 cgtccatgtc gaatgcggtc tgccgcgcgg ggagcaactg gccgagaccg actggttgca
     1021 ggccatggcc gaggcctacg gctatcccca aggcatcgtc gctggggcga acatgctgga
     1081 cccggatctc gacgcccttc tcgaggctca tgcggcacgt ccgaacgtgc gcggcgtgcg
     1141 tcagatcgtc tgttggcacg aggatccgtc gaagacctat accgaccgcg acctgctgct
     1201 ggatcccgcg tgggaggtcg gcttcggtaa gctggcgccc cacgggctgt cgttcgacct
     1261 gcagctctac ccgtcccaaa tggcgacggc ctttgtcatc gccgcgcgcc atcctgacgt
     1321 gcccatgatc gtcaatcacg caggtctgcc gaccgaccgc gacgccgacg ggatgaagcg
     1381 ctggcgcacc ggcttgcgtc tgttggccgc tcagccccaa gtctcgatca agatctcggg
     1441 actggggatc accgaccgcg cctggacggc cgaaaccctg cgtccgatcg tgctcgaatg
     1501 catcgatgcg ttcgggaccg agcgcgccat gttcgcctcc gacttcccgg tcgagcggac
     1561 tcacggatct ttcgaggcct tcttttcagc gttcgatgcg atcacagccg acttcagcgt
     1621 gaccgagcgc gaacggctgt tcgccgccaa cgccgaggcg atctaccgca tctagcttca
     1681 ccatattacc cccgccgaag ccgcgaaaga cggtagggca cacggaaaca ccgaggacgc
     1741 catgagcatc gaaatccacg ccgcccacac acccggaggc ctcgccgccc ccgcaggatc
     1801 gctgaaaggg ccgctggcgt tcgccatcgc ctgcttcctg atctgggggc tggcctacgg
     1861 tctgctcgac gtcctgaaca agcatttcca ggagacgctg agcatcagtc aggcggacag
     1921 ctcgtggctc cagatcgcct atttcggcgc ctatctgctg ctctcggttc cggcgggcat
     1981 gctgctccac gccaagggct acaagttcgg tatcgtcacc ggcctcgcgg tgaccgcgat
     2041 aggcgcgctg ttgttcattc ctgcggcggg cgcaggcgcg ttcatgcctt tcgtcggctc
     2101 gatgttcgtg ctggcggcgg gcctttgcat tctcgagacc tcggcagaca cttacgtcaa
     2161 cgtgctcggc gacccggcca aggcctcgca acggctgaac ctcgcccagt cgttcaacgc
     2221 gctcggcgtc ttcttcggac ccctgatcgg cggcgcggtg ttcttcagcc cgacgacgac
     2281 cgaggccctc ggtggcgcga cccgttcgat ccaggtcgtc tatggcgtga tcgccgtcgg
     2341 cgtcgtgatc ttcgccttcg ccgtctggcg cgcccgcctg cctgagaccg gtctgtcgga
     2401 ccatggcgat gcggtcgccg gcgacgcccc tgcccgcccc ctgtcgcaac agccccactt
     2461 catcgccggc gtcatcacgc aggctttgta catcggcgcg caggtcggga ttggcgccta
     2521 cttcatcaat ctggtcaccc acaactggaa cggtctgaca tcgcagcagg gcgcgttcat
     2581 gctgtcactg gcggccatcg gctatctggt cggacgcttc gccgcgaccg cgctgctgct
     2641 gcgggtcaag ccccgcgtgc ttctgaccac ctacggcctg atcaacgtcg ccctgacgct
     2701 gatcgtcgtc gcggggatcg agcgggtgtc gccgatcgcg ctgatcgcag tgttcttctt
     2761 catgtcgacc atgttcgcga ccatcttcgc cctcggcacg acgggcctcg gcgccggaac
     2821 caagaaggcg tcatcgctga tggtcatggc gatcggcggc ggggttctgc tgccctggcc
     2881 gatgggcaag atcgccgatc tctacggcgc caacatcgcc ttcatcctgc cctgcgtctg
     2941 tttcgcggtg gtcgcctggt acggctggaa aggcgccgtg ctcggccgga cggagcgggt
     3001 ctgatgcgta ttcgctccgc actgctcgcc ttcaccctgc tgaccgctcc ggcggcggcc
     3061 atggcccaga cgacgccggc ggcgatcgtc gctcccgctc atccgatcga ccgtcaggcg
     3121 ctggtgtcac ggcataacgt cgtcctgacc gccatcgatc gccatgctcc gatcatgctg
     3181 ggcaacggcg cgctcggctt caccgccgac atcaccggac tgcagacgtt tcccgagcaa
     3241 tactcggagc tcgcgccgct gatgactatg gcccagtggg cctggcacag cttcccgaac
     3301 cctgagggct acaccgagca gaacggcctc gttcaggtcc cggtgccggg gcgaggtgat
     3361 cagccctatg cctggatgaa gtcctgggcc gatgcggaca ccaacccggc ctacaactgg
     3421 ctgcgggcca atccgcaccg gttctcactg gcccggatcg gtctgatatt gaaggacggt
     3481 caggcgctgg acttcgccaa ggtctccggc gcgacccaga cgctggacct gtggacaggc
     3541 gccctgacca gccgcttcac ctatgacgga cagcccgtcg aggtcgtcac gcgggtgcat
     3601 ccgacgctgg acatggtgat ggtcgagatc gcgtcgccac tggtcgccag tgagggcttg
     3661 ggcgttcagg tccgctatcc tgaggtgaac ccggccatca accctgacac cactctcttt
     3721 gaaggcgatg gcgcgcagag cctgagcgtc gtcgcccacg acacgggcga tatccgcatt
     3781 cggcggactc tggatgagac cacctattat tcctccatca cggcctcggg caaagtcgac
     3841 caaaccggcg ccgacgcctt ccgcgtcgta tcgaccggtc gcgaccggct gaccgtcatg
     3901 gtccgcttcg atcagcaggc cgaggcgcct gcgacgcccg cctacagcgc cggtgttcag
     3961 gccgcgacgg cccaatggca tgacttctgg accaagggcg gcgcggtcga tttctcgggc
     4021 tccaccgatc ctcgtgcggc cgagctcgag cgtcgtgtcg tcctgtccca gtatctgtcg
     4081 gcggtgaacg gcgcaggcgc cttgccgcct caggaagagg gactgttctc caacagctgg
     4141 tacggcaaat tccatctcga gatgcatccc tggcattcgg gctggcaggc gctctggggg
     4201 catccggaga tgctggagcg cagcttgccc tggtatgtcg gcaacctcgc gaacgctcga
     4261 gccgaagccg cgcgtcacca cgttcagggc gcctggtggc ccaagatggt cgggcctgat
     4321 gggcgtaaca gccccagcct cgtgtcgccc tttatcatgt ggcagcagcc gcattcgatc
     4381 ctgctggccg aactggtctg gcgcgccgag aaggatccgg cggtgctggg tcgctatggc
     4441 gaggtagtcg aggcgaccgc cgacctgctg gcctcatggc cgatcgaacg cgatggccgg
     4501 ctgaacctcg gcacgccgat gatcccggcg caggagaact atgatccgct gaccacgacc
     4561 aatcccacgt tcgagctgga gtatttccgc tgggcgctgg agacggcgca ggcctggcgc
     4621 gagcgtaaag gtcagcctcg caaggcggaa tgggatcggg cgctggccaa gttggcgtcg
     4681 ccgccaatga aggacggtct ttatctgccc atcgagaacg cgccggactt ctggaccatc
     4741 gctgcctccg acgcctgccg ccgccatgcc tcagagcctc agt
//
LOCUS       DASVTM010000360         8472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_403484,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000360 DASVTM010000000
VERSION     DASVTM010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403484"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VF633_09790"
     CDS             complement(<1..507)
                     /locus_tag="VF633_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471393.1"
                     /translation="MAARQAASLDHYEGAITANPQAPAVAAENRERSLILRRWLDRQP
                     VITSLAFAAMHDFDNFLEPVGRQRFLTEYYGVKPLHIAATSEALNSPWFDWKRLNALL
                     SQTAIWTPDNLKLLQNGSTVPPERYCVEVATPVGRTLRPSPAKVETYLSRGASLVAND
                     IHTLTPEIA"
     gene            538..1203
                     /locus_tag="VF633_09795"
     CDS             538..1203
                     /locus_tag="VF633_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX8471394.1"
                     /translation="MSSFPTAEQITAARVALAELDPALARAHAGTPVFEWRARIGGYE
                     GLFRMIVEQQVSVASAAAIWSRTVDGLNGEVTPDAVLACDIETLRSFGLSGQKARYGH
                     EIALAQTEGRIEFDHLGRLSDAEATAALVAIKGVGRWTAETFLMFCEGRTDVFPGGDI
                     ALQEAMRWADRAELRPNEKQAYLRAERWRPHRSVAAHLLWGWYGGVKRGEIALEEARM
                     GEI"
     gene            1203..1865
                     /locus_tag="VF633_09800"
     CDS             1203..1865
                     /locus_tag="VF633_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX8471395.1"
                     /translation="MTPLMDPETAKALADPQRLLSVLDFAGVAVFAATGALAAARERH
                     DLVTFAFFAAITGVGGGTLRDLLLGVPVFWVQDWRYIAICIAASVVIWMIGRRDWRFR
                     ALLWLDAIGLGAYGVLGAAKAEAVGAAPLICIVMGALTACFGGIVRDLLAGQPSILLR
                     REITVSAALLAATVYVVLRMPELSPWPAAVIAALCGFGLRAGALRYGWSLPGFPDASR
                     RS"
     gene            2030..2590
                     /locus_tag="VF633_09805"
     CDS             2030..2590
                     /locus_tag="VF633_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8471396.1"
                     /translation="MQALIRPPSGFPALVLNADFRPLSYYPLSLWPWEEAVKAVYQDR
                     VDVVSHYDKIVRSPSMEMQLPSVIALKSYVDQNRSPAFTRFNVFLRDGFACQYCGESA
                     ELTFDHVVPRSHGGRTTWENIVAACSPCNLRKGGRTPRQAQMPIRRLPHRPNAYQLQD
                     AGRRFPPHYLHQSWLDYLYWDIELEP"
     gene            2733..3512
                     /locus_tag="VF633_09810"
     CDS             2733..3512
                     /locus_tag="VF633_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX8471397.1"
                     /translation="MKLRVRAELVYRFDPPTDAIYQIEVAHWPGQDILEESLTTTPEV
                     RFHEDKDEVFGARALRAHLAGDVSLVYEATVDNGSLKGLPTGVRQHDWNELPSETLTY
                     LLPSRYCPSDQFGRFVQRQFGETSGGERVLAILGWIKENVDYEFGVSDTETTAARTFI
                     DRAGVCRDFTHLGITLCRASGIPARAVSAYAHLLDPPDFHAIFEVWLSNGWWLVDPTG
                     LAPIEGLVRIACGRDAADIAFLSTQGACEMISQSITARAID"
     gene            complement(3513..5240)
                     /locus_tag="VF633_09815"
     CDS             complement(3513..5240)
                     /locus_tag="VF633_09815"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX8471398.1"
                     /translation="MFLLKSFLPCGLALGVAVLIPACASAADSTAFTLTTGTERTPEQ
                     LVTRFDKADLELKVLPQSKTIEGVATLDLTATGRLAHVVLEFDTVFNVSSVSVDGQAL
                     AASQWSNPDGRMTITLPLPLTAGATASVRIAYSGSPHRAKNAPWDGGFVWSTTPDGQP
                     WIATAVQGEGCDLFWPCIDHPQGEPARVDLHITVPSELSAPSNGRFLGKIDNGDGTTT
                     WNWTAAHPDTYAIALNVGPYVEMTADYRSRFGNVVPLHFWRLATDRQDRAEALFAEFP
                     KMLDFYEAEVGPFPFGDEKMGVVETPHLGMEHQTINAYGNAYKLDGKGYDWLLQHELA
                     HEWFGNQMTNASWDDMWLHEGLGTYMQPLYARWLNGDRYMEAELATMRLTLKNNFPLV
                     SGRVQDSGTVYTDATGPGLDLYYKGALVAHSLRLLIGDEAFQRSLTRLVYGRPDPRPG
                     NFEALYASTPDFIRIVEEESHRHLGWFFSTYLYQAALPQLEMTRNGDRVSLNWINAAG
                     AFPMPLEVEVDGQVRSLAMRDGHDAFTAPAGAHILIDPHNKVLRQLDFVDAYQARQRE
                     ESGAPQPRH"
     gene            complement(5335..6321)
                     /locus_tag="VF633_09820"
     CDS             complement(5335..6321)
                     /locus_tag="VF633_09820"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEX8471399.1"
                     /translation="MFGKRSGLAILVCAGALLSAAGQACARADDMPLGQLASPPTGYI
                     DMCERTPVLCVQTPNATPSQLDAVRRWAAQTRWSVIFARRLGVPTLDAVSRSERATAA
                     TGPASDVVGEAPLPLPASQQASASTAGSPIPATVANPQSLALVASPRRWDLARTRAVV
                     SVAPRPVEAASPAAGAASPPAQADKTTPGEDWQPPRFFADLGMDQLNEINRTVNRAIR
                     PTSDLAAFGKVEYWTIPRGSGAAGDCEDYVLAKRQALIQAGAPVESLSIAVVQTSRRE
                     MHTVLLVATRDGEVVLDNLTPWIVSWRAAPYTWLQRQARGSALTWVYSGLSA"
     gene            complement(6694..7995)
                     /locus_tag="VF633_09825"
     CDS             complement(6694..7995)
                     /locus_tag="VF633_09825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX8471400.1"
                     /translation="MRVTMIGTGYVGLVSGACFADFGHTVTCVDKDPSKIERLNGGEI
                     PIFEPGLDDLVAKNVKAGRLSFTLDGAEAIRDAEVVFIAVGTPTRRGDGHADLSYVYA
                     AAEEIAGLIDGFTVVVTKSTVPVGTGDEVEAIMRRVRPDGEFAVVSNPEFLREGAAIE
                     DFKRPDRVVIGTEDPKARAVMGELYRPLSLSDFPVVYTARRTSELIKYAGNAFLAMKI
                     TFINEMADLCEKVGADVQQVAKGIGLDGRIGSKFLHAGPGYGGSCFPKDTIALVRTAQ
                     QYGAPVRLIETTVEVNDARKKAMAKRVEAMIGDLNGKTVGILGLTFKPNTDDMRDSPS
                     LDIIPALQAMGAKVKAFDPEGMKEAAHLFTDLELVDGPYAAAQGADALILLTEWDQFR
                     ALDLARIKDSMNQPVLLDLRNVYKPAEARAAGFTYSSIGRA"
     gene            8170..>8472
                     /locus_tag="VF633_09830"
     CDS             8170..>8472
                     /locus_tag="VF633_09830"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEX8471401.1"
                     /translation="MLFGDSHAAQWFTPVAAFATSQDAVLTTVLKSSCPPAAVDVVSS
                     RLRRIFTECLTWRVAALEQIRALHPDLVIISEHSLSYVRRPDFEVSAPSATPDEWRR"
BASE COUNT         1439 a         2610 c         2789 g         1634 t
ORIGIN      
        1 tgcgatttcc ggcgttagcg tatggatatc gtttgcaacc aagcttgccc cccgtgacag
       61 gtaggtctcg accttcgccg gtgagggccg gagcgtccgc ccaacagggg tggcaacctc
      121 tacacaatac cgctcaggcg gcacggtgct gccgttctga agaagcttta ggttatctgg
      181 cgtccagatt gcggtctgcg acagaagggc atttagccgc ttccagtcaa accacgggct
      241 gttgagcgct tccgacgtcg cggcaatatg gaggggctta acgccgtagt attccgtcag
      301 gaatcgctgg cgacccacag gctccaagaa attatcgaaa tcatgcatgg cggcgaacgc
      361 tagtgaggtg atgacgggtt gtcgatccag ccatctccgc aaaatcagcg atctctcgcg
      421 attttcggcc gccacggccg gagcttgcgg attagcggtg atcgcgcctt cgtagtggtc
      481 cagactagcc gcttgtcgag cagccaaagt cggtctgcct catctgtctg aggtgccatg
      541 tcctccttcc ccactgctga acaaataacc gccgcccggg tggcattggc ggaactcgac
      601 ccggctctgg cgcgcgccca tgccgggacc cccgtgttcg aatggcgggc ccggatcggc
      661 ggctatgagg gcctgttccg gatgatcgtc gagcagcagg tctctgtcgc ttcagccgcc
      721 gcgatctgga gtcgaacggt cgatggtctg aacggcgagg tgacgcctga tgcggtgctg
      781 gcgtgcgata tcgagacgct gcggtccttc ggactgtcgg ggcagaaggc gcgttatggc
      841 cacgagattg cgctcgccca gaccgagggc cggatcgaat tcgaccatct gggacggctt
      901 tcggacgccg aggcgacggc ggcgctggtg gcgatcaagg gtgtcggtcg ctggaccgcc
      961 gagaccttcc tgatgttctg cgaagggagg accgacgtct ttcccggcgg cgacatcgcg
     1021 ctgcaggaag ccatgcgctg ggctgaccgg gccgagttgc gacccaatga gaaacaggcc
     1081 tatctgcgcg ctgaacgctg gcgaccgcac cggagcgtgg cggcgcatct gctgtggggt
     1141 tggtacggcg gggtcaagcg tggcgagatc gcgcttgaag aagcgaggat gggggagatc
     1201 tgatgacgcc gctgatggac cccgagacgg cgaaggcgct ggccgacccg cagcggctgc
     1261 tgtcagtgct ggatttcgca ggcgtcgccg tgttcgccgc gaccggcgcc ttggcggccg
     1321 cacgtgagag gcacgacctc gtcaccttcg cctttttcgc ggccattaca ggggtcgggg
     1381 gcggcaccct gcgcgacctg ctgctcggcg ttccggtctt ctgggttcaa gactggcgct
     1441 acatcgccat atgcatcgcg gcttcggtgg tcatctggat gatcggccga cgcgattggc
     1501 gcttcagggc cctgctgtgg ctcgatgcga tcggcctcgg cgcctacgga gtactcggcg
     1561 cggccaaggc cgaggcggtc ggcgccgcgc cgctgatctg catcgtcatg ggcgctctga
     1621 ccgcctgttt cggcggcatc gtgcgggact tgctggcggg tcagccctcg atcctgcttc
     1681 ggcgcgagat caccgtttcc gcggccttgc tggcggcgac tgtctatgtg gtgctgagaa
     1741 tgccggagct ttcgccttgg cctgccgccg tgattgcggc cttgtgtggc ttcggcctgc
     1801 gcgccggcgc gctgcgatat ggctggagcc tgcccgggtt tccggacgca tcgcgccgaa
     1861 gttgatgaag agtctatcac gaacttaggt ggaaaatcgt gatcgatccg ccggcggaac
     1921 gtcatggcgg cgtcgtcgaa tctggcctaa cgtaccgaca cgaataagga aggagacgcg
     1981 tcttcagtcc tgtcgcctca caccacctcc agccttgcgg gagggcctca tgcaggcctt
     2041 gatccgacca ccgtccggtt ttccggcgct ggtgctgaac gctgatttca ggccgctgtc
     2101 ctattacccg ttgtcgttat ggccttggga agaggcggtg aaagccgtct atcaggatcg
     2161 cgttgacgtc gtttctcatt atgacaagat cgtgcgcagc ccctctatgg agatgcagct
     2221 gcccagcgtg atcgcgctga agagctatgt cgaccagaac cgcagccccg ccttcacccg
     2281 tttcaacgtt ttcctgcgcg atggttttgc ctgccagtat tgcggcgaga gcgccgagct
     2341 gaccttcgac cacgtggtgc cgagatccca tggtggccgt accacctggg aaaacatcgt
     2401 cgccgcctgt tcgccctgca atctgcgcaa aggcggccgc actccgcgtc aggcccagat
     2461 gccgatccgg cgcctgccgc accgcccgaa tgcctatcag ctacaagacg caggccgccg
     2521 gtttccgccg cattatctgc accagagctg gctcgactat ctctactggg acatcgagct
     2581 cgaaccgtaa aagattgcgc gatgccgctt tttcgcactg cgtggtctgg tgctatagtc
     2641 ggcccaagcg ctaaaccgcg ccccacggaa cgggcgcaac tgcgtgccgt tcagcagccg
     2701 tatttccccg tctttgaaag tttcgtccgc ccatgaagct gcgtgtccgc gccgagcttg
     2761 tctatcgttt cgacccgccg acggatgcga tctaccagat cgaggttgcg cactggccgg
     2821 gtcaggacat tctcgaggaa agcctaacga ctacgccgga ggtaaggttt catgaggaca
     2881 aggacgaggt attcggcgcc cgggccctga gggcgcatct cgcaggcgac gtttctctgg
     2941 tctacgaggc gacggtcgac aacgggtccc tgaaggggtt gccgacagga gtgcgccagc
     3001 atgactggaa cgagcttccg tcagagacgc tgacctatct gctgcccagc cgctattgtc
     3061 cgtcggacca gttcggtcgc ttcgttcagc gtcagttcgg cgagacctcg ggcggcgagc
     3121 gcgtgctggc gatcctcggc tggatcaagg agaacgtgga ctacgaattc ggtgtttcgg
     3181 acaccgagac gacagcggct cgcaccttca tcgaccgagc cggagtttgc cgcgacttca
     3241 cgcatctggg gatcaccctg tgccgggcgt ccggtatccc ggcgcgagcg gtaagcgcct
     3301 atgctcatct gctggatccg ccggacttcc acgccatttt cgaggtctgg ttgtcaaacg
     3361 gctggtggct ggtcgatccg acgggcctgg cgcctatcga aggtctggtg cggatcgcct
     3421 gcgggcgcga cgccgccgac atcgcctttt tgagcaccca gggcgcctgc gagatgatct
     3481 cgcagtcgat cactgcgagg gcgatcgact agctagtggc gaggctgcgg cgcgccggat
     3541 tcttcgcgct gacgagcttg ataggcgtcg acgaaatcga gctggcgcag gaccttgttg
     3601 tgcggatcga tcaggatgtg ggcgcctgcc ggtgcagtga aggcgtcgtg gccatcgcgc
     3661 atcgccagtg agcggacctg accgtcaacc tcgacttcca ggggcatggg gaaggcgccg
     3721 gcagcattga tccagttcag actgacccgg tcgccgttcc gcgtcatctc gagctgcggg
     3781 agcgcggcct gatagagata ggtcgaaaag aaccagccga gatgcctgtg gctctcttct
     3841 tcgacgatgc ggatgaagtc cggcgtcgag gcgtagagcg cttcgaaatt gcccggccgc
     3901 ggatcggggc gaccgtagac cagccgggtc agagaccgct ggaatgcctc gtccccgatc
     3961 agcagccgca gcgaatgagc gaccagtgcg cccttgtaat agaggtcaag accggggccg
     4021 gtagcgtctg tgtaaaccgt gccggagtcc tgaacgcgtc ccgaaaccag cgggaaattg
     4081 ttcttcaacg tcagccgcat ggtcgccagc tcggcctcca tatagcggtc gccgttcagc
     4141 cagcgcgcgt aaagtggctg catataggtg ccgagcccct cgtgcagcca catgtcgtcc
     4201 cacgacgcat tggtcatctg gttgccgaac cattcatggg ccagctcgtg ctgcagcagc
     4261 cagtcgtagc ctttgccgtc cagcttgtag gcgttgccgt aggcgttgat ggtctggtgc
     4321 tccatgccca gatgcggggt ctcgaccacg cccatcttct cgtcaccgaa cgggaagggg
     4381 ccgacctcgg cctcgtagaa gtcgagcatc ttcggaaatt cggcgaacag ggcctcggcg
     4441 cggtcctgac gatcggtggc caaccgccag aaatgcagcg gcacgacatt gccgaagcgg
     4501 ctgcggtagt ccgcggtcat ctcgacatag ggaccgacgt tgagcgcgat cgcataagtg
     4561 tccggatgcg ccgccgtcca gttccaggtc gtcgtcccgt cgccattgtc gatcttgccg
     4621 agaaagcgac cgttggacgg cgcggacagc tccgatggga cggtgatgtg cagatcgacg
     4681 cgtgccggct cgccctgcgg atggtcgatg cagggccaga acaggtcgca gccctcgccc
     4741 tgaactgcgg tggcgatcca tggttggccg tcaggcgtcg tcgaccagac gaagccgccg
     4801 tcccaaggcg cgttcttcgc ccggtgcggc gatccggaat acgcgatgcg cacagaggcg
     4861 gtcgctcccg cggtcagcgg taggggcagg gtgatggtca ttcgcccatc ggggttggac
     4921 cattggctgg cggcaagcgc ctgtccgtcg accgaaaccg aggacacgtt gaagacggta
     4981 tcgaactcca gaaccacgtg ggcgaggcgg ccggtcgctg tcaggtcaag tgtcgcgacg
     5041 ccttcgatcg tcttcgattg cggcaagact ttcagttcca gatcggcctt gtcgaaacgc
     5101 gtgaccagct gttccggcgt ccgttccgtc ccggtcgtca gggtgaaagc ggtgctgtcg
     5161 gctgcagagg cgcaggccgg gatcaggacg gcaacgccga gcgcgaggcc gcacggcagg
     5221 aaagacttga gcaggaacat tgcgactcca gacaggatcg acgaccctta cgattttcgc
     5281 aatcgaggtg tccaaccgtt ggcgtccgca agtctggtca cggcttggaa atgcctaggc
     5341 cgagaggccc gaatagaccc acgtcagggc cgagccgcgc gcctggcgct gaagccatgt
     5401 gtagggagct gcgcgccagg atacgatcca cggggtcagg ttgtcgagta cgacctcgcc
     5461 gtcccgcgtg gcgaccaaaa gcacagtgtg catttcgcgc cggctcgtct gcaccacggc
     5521 aatggacagc gattctaccg gtgcgcccgc ctggatcagg gcttggcgct tggcgaggac
     5581 atagtcctcg cagtcgccag ccgcgcccga acctcgcgga atcgtccagt attcgacctt
     5641 gccgaaggcg gcgaggtcgc tggtgggcct tatggcgcgg ttaactgtcc tgttgatttc
     5701 gttgagctga tccatgccga gatcggcaaa gaagcgcggc ggctgccagt cttcgcccgg
     5761 cgtcgtcttg tccgcctggg caggcggcga cgctgcgccc gccgccgggg aagccgcttc
     5821 caccggcctc ggtgcgaccg aaacgactgc tcgggttcgg gccaggtccc accgacgtgg
     5881 gctcgcgacg agcgccagcg attgcggatt cgctacagtc gccgggatcg gcgaccctgc
     5941 ggttgaagcc gaagcctgtt gagaggcggg aagaggaagg ggcgcctcgc cgacaacgtc
     6001 ggaagcaggc ccggtcgcgg ctgtcgctct ttcgctgcga ctgaccgcat caagcgtcgg
     6061 gacgcctaac ctgcgagcga agatcacgct ccaccgggtc tgggcggccc accggcgcac
     6121 ggcgtcaagt tggctgggcg tggcgttcgg ggtctggaca cacaggacgg gggtccgctc
     6181 gcacatgtcg atatagccgg tcggcggtga tgccagctgc ccgaggggca tgtcgtcggc
     6241 gcgggcgcag gcctgtccag ccgcgctcag cagggcgccg gcacagacca gtattgctaa
     6301 tcccgaacgc ttcccgaaca tcagttattc ctcgatccta cgaggagata ctgacaggtc
     6361 ggttataacg tgcgttttat actgttggtt tgttttttat gaagtcttat gcttttcagg
     6421 cggtaaggtt aatgcgccaa aaaccttgat cgttgttgtg cccgcgtctg gggaagtcag
     6481 gccgggaggg gagcgcagcg gcaagtcaac ctccggacgc ccggtcatga ccgcagcccc
     6541 ggttcacagc ctacgtacgc cgtcggtcga cgcgcaggat gcgatccggg gctgacaacg
     6601 aaagcgtcag ccggcctgta acgcctcgag ccgcatcccg cccggcgacc gcgtgctaac
     6661 ggcgtgagaa cgccttgcac acgcgatatc aggtcaagcg cgcccgatgc tggagtaggt
     6721 gaagcccgca gcgcgcgctt cggcaggctt gtaaacgttt cgcagatcca gcagcaccgg
     6781 ctggttcatg ctgtccttga tcctggccag atcaagcgcg cggaactggt cccactccgt
     6841 cagcaggatc agggcgtcgg cgccctgggc tgcggcatag gggccatcga ccagctccag
     6901 gtcggtgaac aggtgggcgg cttccttcat gccctcaggg tcgaaggcct tgaccttggc
     6961 gcccatggct tgcaacgccg ggatgatgtc caggctgggg ctgtcgcgca tgtcgtcggt
     7021 gttgggtttg aaggtcaggc ccagaatgcc gaccgttttg ccattcaggt cgccgatcat
     7081 ggcctcgacc cgcttggcca ttgccttctt gcgcgcgtca ttgacctcaa cggttgtctc
     7141 gatcaggcgg accggcgcgc cgtactgctg ggccgtgcgg acaagggcga tggtgtcctt
     7201 ggggaagcag gagccgccgt atcccgggcc cgcgtgcagg aatttcgacc cgatccggcc
     7261 atccaggccg atccccttgg cgacctgctg gacgtccgct ccgacctttt cgcacaggtc
     7321 ggccatctcg ttgatgaagg tgatcttcat cgcgagaaag gcgttgcccg cgtatttgat
     7381 cagctcggag gtacggcggg ccgtgtagac gaccgggaaa tcgctgaggc tcagcggacg
     7441 gtacaattcg cccatgaccg cgcgcgcctt ggggtcctcg gtgccgatga cgacgcggtc
     7501 cggccgcttg aagtcctcga tcgcggcgcc ttcgcgcaga aactcggggt tggagacgac
     7561 ggcgaactcg ccgtcgggcc gcacgcgtcg catgatcgcc tcgacctcgt caccggtgcc
     7621 gaccgggacg gtggatttcg tgaccaccac ggtgaagccg tcgatcagtc cggcgatctc
     7681 ctcggcggcg gcgtagacat aggaaaggtc ggcatggccg tcgccacggc gtgtcggcgt
     7741 gccgacggcg atgaatacga cttcagcgtc acggatggcc tctgcgccgt cgagggtgaa
     7801 cgacagccgt cccgccttga cattcttggc caccagatcg tccaggccgg gttcgaagat
     7861 cggaatctcg ccaccgttca gccgctcgat cttggacgga tccttgtcga cgcaggtgac
     7921 cgtatgcccg aagtccgcga agcaggctcc gctcaccaga cccacatagc ctgtgccgat
     7981 catcgtgacg cgcatggttc atattccccg ataaagccga gctctaccta gccagtagta
     8041 tgttgcgctg caataagagg tttgggaggc ggtgcagatt cccgatttgc gcccttgtag
     8101 cgatgggacc tggagtggga accggatttg ggcgtcgtgt tagccctatt cctctcgggt
     8161 cgcagggtcg tgctattcgg cgactcgcat gctgcccaat ggttcacgcc cgtcgcggct
     8221 ttcgccacaa gccaggacgc cgtgctgacg acggtgctga aatcctcgtg cccgccggcc
     8281 gcggtcgatg tggtcagttc ccgtctacga aggatcttca ccgagtgcct cacctggcga
     8341 gtagccgcgc tcgaacaaat ccgcgcgctg catcctgacc tcgtgattat ctcagagcat
     8401 tccctgagct atgttcgtcg tccggatttc gaggtttcgg cccccagcgc tactccggac
     8461 gaatggcgtc gg
//
LOCUS       DASVTM010000361         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_939879,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000361 DASVTM010000000
VERSION     DASVTM010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939879"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VF633_09835"
     CDS             complement(<1..222)
                     /locus_tag="VF633_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8471402.1"
                     /translation="MSFAFDAQVTAALFDKDGAVFTLGDGSVRFEDGSYNQVHDGAVL
                     CAALHPSGEGLVTGGDDGRVIWSRKNEAGV"
     gene            complement(219..1340)
                     /locus_tag="VF633_09840"
     CDS             complement(219..1340)
                     /locus_tag="VF633_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8471403.1"
                     /translation="MTETLDAPASPAAVANGKIPVTVLTGYLGAGKTTLLNRILTEDH
                     GKRYAVIVNEFGEIGIDNDLVVGADEDVFEMNNGCVCCTVRGDLIRVVAGLMKRQRPG
                     KPAFDAIIVETTGLADPGPVAQTFFVDEDVKAKTQLDSVTALVDAKHVMARLDDSKEA
                     REQVAFADRIILNKVDLVLEAELVVVEQRLRKLNPLAPIIRAERANVPLDQVLGLHGF
                     DLERILDLNPEFTNPGHGEAGHVHDEHCGHGHHDHAGHDHAHHDHDHDHGARGHAHED
                     DIKGVSVSVDGPMNGVKFTAWLDKLLGEQGQNILRAKGIIDVQGEDRRLVFQAVHMIL
                     EGDLQREWKAGERRWSRAVFIGRDLDEAALKAGFESCAA"
     gene            complement(1397..2269)
                     /gene="aguB"
                     /locus_tag="VF633_09845"
     CDS             complement(1397..2269)
                     /gene="aguB"
                     /locus_tag="VF633_09845"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEX8471404.1"
                     /translation="MPRTISVAGLQTSYGEDMGANIRKTADLVRQAASKGAQVILPSE
                     LFQGPYFCVSQEEHWFSTAYPWREHPAVIAMAEVAKDLGVAIPVSIFEKEGPHYYNSI
                     VMLDADGSPLGLYRKSHIPDGPGYQEKYYFRPGDTGFKVWDTRFGKVGVGICWDQWFP
                     EAARAMALMGADVLMYPTAIGTEPHDANLNTAQPWRRAMQGHAVSNAIPVVGANRIGH
                     EAVTLVGQTFYGHSFIADQQGELVESLGAEEEGSVVHTFDLAELDRYRAAWGFFRDRR
                     TDLYGTLASPRPTS"
     gene            complement(2269..>2814)
                     /locus_tag="VF633_09850"
     CDS             complement(2269..>2814)
                     /locus_tag="VF633_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX8471405.1"
                     /translation="ILTTRQCLLNPNRNTGWTEQAASIALTEALGARKIVWLGDGLLN
                     DHTDGHVDNLARFVAPGVVAVPMAFGTNDPNTEAYENAAAAVRAATDAEGTPLKVLLI
                     PSPGFIADEDERPIPASHMNFLIANGAVIVPTYGTAMAARLACEALATVFPDREIIPL
                     SSNAILTGGGSFHCISQQEPA"
BASE COUNT          472 a          878 c          961 g          503 t
ORIGIN      
        1 cacgcccgcc tcgttcttgc ggctccagat cacccgaccg tcgtcaccac cagtgaccag
       61 accttcgccg gaaggatgca gggcggcgca caacaccgcg ccgtcgtgga cctgattgta
      121 ggatccgtcc tcgaaccgga ccgacccgtc gccgagcgtg aagacggcgc cgtccttgtc
      181 gaacagggcg gcagtgacct gggcgtcgaa cgcgaaactc acgctgcgca gctctcgaaa
      241 ccggccttga gtgcagcttc gtcgaggtcg cgtccgatga agaccgcgcg gctccagcgg
      301 cgttcgcccg ctttccactc gcgctgcaga tcgccctcga ggatcatgtg gacggcctgg
      361 aacaccagac ggcggtcttc gccctgaacg tcgatgatgc ccttggcgcg caggatgttc
      421 tggccctgct cgcccagcag cttgtcgagc caagcggtga atttcacgcc gttcatcggc
      481 ccgtcgaccg agaccgagac gcccttgata tcgtcctcgt gcgcatggcc gcgcgcgcca
      541 tggtcgtggt cgtggtcatg atgagcatgg tcgtgaccag catggtcgtg atggccgtgg
      601 ccgcaatgtt cgtcgtggac gtggcctgcc tcgccatgac ccgggttggt gaactcgggg
      661 ttcagatcga ggatacgttc aagatcgaag ccgtgcagac ccagcacctg atccagcggc
      721 acattggcgc gttcggcgcg gatgatcggg gccagcgggt tgagcttgcg caggcgttgc
      781 tcgaccacaa ccagttcggc ctctaaaacc agatcgacct tgttgagaat gatgcggtcg
      841 gcgaaggcca cctgctcgcg cgcctccttg gagtcgtcca gacgggccat aacgtgcttg
      901 gcgtcgacca gagcggtgac gctgtcgagc tgggtcttgg ccttgacgtc ctcgtcgacg
      961 aagaaggtct gggccaccgg gccgggatcg gcgaggcctg tggtctcgac gatgatggcg
     1021 tcgaacgcag gcttgccggg gcgttggcgc ttcatcagcc cagccaccac gcggatcagg
     1081 tcaccgcgta ccgtgcagca gacgcagccg ttgttcatct cgaacacgtc ctcgtcggcc
     1141 ccaaccacga ggtcgttgtc gatgccgatc tcgccgaact cgttgacgat gacggcgtaa
     1201 cgcttgccgt ggtcttcggt caggatgcga ttcaacaacg tcgtcttgcc cgcaccgagg
     1261 tagccggtca ggacggtgac ggggatcttg ccgttagcaa cagcggcggg cgaggcgggg
     1321 gcgtcgaggg tctcggtcat aggccgctag atggcggttc agaccctcga agggaagagg
     1381 cgcccgccgc ccgttttcag ctcgtgggac gcggcgaagc cagcgtaccg tacaggtccg
     1441 tccgacgatc tcggaaaaag ccccatgcgg cacggtagcg gtccagctcg gcgaggtcga
     1501 aggtgtggac caccgatccc tcctcctcgg ccccaaggct ttcgaccagt tcgccctgct
     1561 ggtcggcgat gaaggagtgc ccgtagaagg tctggccgac cagagtcacc gcctcatggc
     1621 cgatccggtt ggcgccgacg accgggatgg cgttcgacac cgcgtggccc tgcatggcgc
     1681 gccgccaagg ctgggccgtg ttcaggttcg catcatgcgg ctcggtgccg atcgcggtcg
     1741 gatacatcag aacatcggcg cccatgagcg ccatggcccg cgccgcctcg gggaaccact
     1801 ggtcccagca gataccgacg cccaccttgc cgaagcgcgt gtcccaaacc ttgaagcccg
     1861 tatcgccggg gcggaaatag tatttctcct gatagccggg gccgtcggga atgtggctct
     1921 tgcgatagag gccgagcggc gatccgtcgg cgtcgagcat gacgatcgag ttgtagtagt
     1981 gcggaccttc cttctcgaag atcgacaccg ggatggcgac gccgaggtcc ttggcgactt
     2041 cggccattgc gatcaccgcc gggtgttcgc gccagggata ggccgtgctg aaccagtgct
     2101 cctcttgcga gacgcagaaa tacggtccct gaaatagctc agaaggcagg atgacctgcg
     2161 cacccttgga cgccgcctga cgcacgaggt cagccgtctt cctgatgttg gcgcccatgt
     2221 cctcgccgta ggacgtctgc aggccggcga cggagattgt gcggggcatc aggcgggctc
     2281 ctgctgggaa atgcagtgga acgatccgcc gcccgtcagg atggcgttcg acgacagggg
     2341 gatgatttca cggtcgggaa agacggtggc caaggcttcg caggccagac gggcggccat
     2401 ggcggttccg taggtcggga caatcactgc gccgttggcg atgaggaaat tcatgtggct
     2461 ggccgggatc gggcgctcgt cctcgtcggc gatgaagccc ggcgagggga tcagcagcac
     2521 cttcagcgga gtaccctcgg cgtcggtcgc ggcgcgcacg gcggcggcgg cgttctcata
     2581 ggcctcggtg ttgggatcgt tggtaccgaa ggccataggc acggctacga cgcccggcgc
     2641 gacgaagcgg gcgaggttgt cgacgtgccc gtcagtgtgg tcgttcagga gaccgtcgcc
     2701 gagccagacg atcttgcgcg cacccagcgc ctcggtcagg gcgatgctgg cggcctgctc
     2761 ggtccagccg gtgttgcggt tggggttcaa caggcattgg cgcgtggtca ggat
//
LOCUS       DASVTM010000362         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_990565,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000362 DASVTM010000000
VERSION     DASVTM010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990565"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="purE"
                     /locus_tag="VF633_09855"
     CDS             complement(<1..340)
                     /gene="purE"
                     /locus_tag="VF633_09855"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX8471406.1"
                     /translation="MATSSTPVAIIMGSRSDWPTMKHAADALDALGVGWEAKVVSAHR
                     TPRRLYEVATGAKAAGFKLIIAGAGGAAHLPGMAASMTDLPVLGVPVESKAMKGMDSL
                     LSIVQMPGGVP"
     gene            465..1028
                     /locus_tag="VF633_09860"
     CDS             465..1028
                     /locus_tag="VF633_09860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8471407.1"
                     /translation="MSVAADIDLIAEVERLRAEVEALRLRAEAAEAAADHDVLTPALN
                     RRGFVAAMNRTMAYCTRYDAPAVILYLDLDGFKGVNDRLGHAAGDAALVRVAELLLAN
                     VRESDAVGRLGGDEFALLMLNAGIEEGRDKARRLAEALTTDGFDWNGEKAELGGSFGV
                     RAWSQQPDAETWLAEADAAMWVRKRSR"
     gene            1130..2533
                     /locus_tag="VF633_09865"
     CDS             1130..2533
                     /locus_tag="VF633_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX8471408.1"
                     /translation="MSIADAPTLSKPSGSLLDLIGKTPMVEVTRIDTGKCRLFLKLEA
                     QNPGGSIKDRIAVSMIEAAEKEGWLKPGGTIVEATAGNTGLALTLVGQNKGYKVLLVI
                     PDKMSKEKIQHLRAMGADVRLTRSDVPHGHPDYYTDMAERLAQQIPGGYYVNQFANHA
                     NSAAHVKTTGPEIWEQMDHDLDAYVAGIGSGGTITGVAQYLKSVGSKAEIILADPVGS
                     TLAGIVNEGVPGPEGSYTVEGIGQNFVPDTADMDLIDKAYSIPDAEAIATVRELLLKE
                     GILAGSSSGTLIAAALRWCREQTEPKRCVTFVCDTGAKYLSKVYNDAWLADQGLGSDP
                     MVGNLSDLITRKYANGDVVVAGPDDSLDSAFKRMRSNDVSQLPVIQEGRLVGILDESD
                     IVHIMNTDEVTRQARFAKPVSEAMTRNLDTVQVTEPLDALIPLFDRDRVAIVLDGETF
                     VGLIARVDLINHLSLNR"
     gene            2592..>2789
                     /locus_tag="VF633_09870"
     CDS             2592..>2789
                     /locus_tag="VF633_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471409.1"
                     /translation="MKLPLSIMPVERGERFEDPIDAELQRTHLGSVSGGGTLMSAPDP
                     EGNKSILYCGVDVDADDLDRAR"
BASE COUNT          501 a          894 c          926 g          468 t
ORIGIN      
        1 caggaacgcc gccaggcatc tggacgatcg acagcaggct atccatgccc ttcatcgcct
       61 tggattccac cggcacgccg agcacgggca ggtcggtcat ggacgccgcc atgcccggca
      121 gatgggccgc gccgccggcg ccggcgatga tcagcttgaa ccctgctgcc ttggcgcccg
      181 tggccacttc atagaggcgt ctgggggtgc ggtgggccga gacgaccttg gcttcccagc
      241 cgacgccgag tgcatcgagc gcgtctgcgg cgtgtttcat cgtcggccaa tcggaccggc
      301 tgcccatgat gatcgccacc ggcgtcgagg atgtcgccat agcaaggctc cagtcgcgga
      361 aagcggcccg ttacagcaca cgcgttgcgc ccgcaaccaa gcgcgctagc atgaccgtta
      421 tcagccgacc cagtgagtcg cgctctcctg gagtttcgcc gaccgtgagt gttgcggccg
      481 acatcgatct gatcgccgaa gtcgagcgct tgcgcgccga agtcgaggct ttgcgcctgc
      541 gcgctgaggc cgccgaagcg gcggcggatc atgacgtcct gacacccgcc ctgaaccggc
      601 gcggcttcgt ggcggcgatg aaccggacca tggcctattg cacgcgttac gatgcgccgg
      661 cggtgatcct ttatctcgac ctcgacggat tcaagggcgt caacgaccgg ctggggcatg
      721 ctgcaggaga cgccgcgctg gtccgggttg cggagctgct gctggccaat gtgcgcgagt
      781 cggacgccgt gggacggctg ggcggggacg agttcgccct cttgatgctc aatgccggga
      841 tcgaggaggg gcgcgacaag gcacgtcgtc tcgccgaggc gctcaccacc gacggtttcg
      901 actggaacgg cgagaaggcc gagctcggcg ggtcgttcgg cgtacgcgcc tggagccagc
      961 agcccgatgc ggaaacctgg ttggccgagg ctgacgccgc gatgtgggtc cggaaacggt
     1021 cgcgctgacg gtgcgttgtg gtctttcggg gcacgatctt gcgccgcttg acgttctgag
     1081 gccgtgcgat cagcttccgc ttcactagcc cctaacggag acctccctca tgtcgatcgc
     1141 tgatgcgccg accctcagca agccctcggg ctccctgctg gacctgatcg gcaagacgcc
     1201 gatggtcgag gtgacgcgga tcgacaccgg caaatgccgg ctgttcctga agctcgaagc
     1261 gcagaaccca ggcggctcga tcaaggaccg tatcgcagtg tcgatgatcg aggcggccga
     1321 gaaggaaggc tggcttaagc cgggcggcac gatagtcgag gcgacggcgg gcaacaccgg
     1381 tctggccctg acgctggtcg gccagaacaa gggctacaag gtcctgctgg tcatccccga
     1441 caagatgtcc aaggagaaga tccagcacct gcgggccatg ggcgccgacg tgcgcctgac
     1501 ccgttcggac gtgccgcacg gccacccgga ctactatacc gacatggccg agcgtctggc
     1561 ccagcagatc ccgggcggtt actacgtcaa ccagttcgcc aaccatgcca actcggcggc
     1621 ccacgtgaag acgacggggc cggagatctg ggagcagatg gatcacgacc tcgacgccta
     1681 cgtcgcaggc atcggttcag ggggaacgat cacgggcgtt gcccagtatc tcaagagcgt
     1741 cggatcgaag gccgagatca tcctcgccga tccggtcggc tcgacgctcg cgggcatcgt
     1801 taacgagggc gtgccgggac ccgagggcag ctatacagtc gagggaatcg gccagaactt
     1861 cgtgccggac accgccgaca tggacctgat cgacaaggcc tattcgatcc ccgacgccga
     1921 ggcgatcgcc accgtgcgtg agttgctgct caaggaaggc atccttgccg ggtcatcgtc
     1981 gggcaccctg atcgctgccg ccctgcgctg gtgccgcgaa cagaccgagc cgaagcgctg
     2041 cgtgaccttc gtctgcgaca cgggcgccaa gtatctgtcc aaggtctata acgacgcctg
     2101 gctggccgat cagggtctgg gaagcgaccc tatggtcggc aacctgtccg acctgatcac
     2161 ccgcaaatac gccaatggcg acgtggtcgt ggcggggcct gacgacagcc tcgacagcgc
     2221 cttcaagcgg atgcgctcta acgatgtgtc gcagctgccg gtgattcagg agggccgtct
     2281 ggtcggcatc ctcgacgaga gcgacatcgt ccacatcatg aacaccgacg aagtgacccg
     2341 tcaggcgcgc ttcgccaagc cagtgtcgga ggcgatgacc cgcaacctcg acacggttca
     2401 ggtgaccgag ccgctggacg ccctgatccc gctgttcgac cgtgaccgcg tggccatcgt
     2461 tctggacggc gagaccttcg tcggcctcat cgcccgcgtc gacctgatca accatttgag
     2521 cctgaaccgg tagtatgggc tggctggaca agcttcgcgg ctcacggcgc aaggagccgg
     2581 gtttcatcta tgtgaagctg cctttgtcga tcatgcctgt tgagcggggc gaacgtttcg
     2641 aagaccctat cgacgctgag ctgcagcgaa cccatctggg ctctgtctca ggaggcggta
     2701 cgctgatgag cgcgcccgac cccgagggca acaagtcgat cctgtactgc ggcgtcgatg
     2761 tcgatgccga cgatctcgac agagcgagg
//
LOCUS       DASVTM010000363         5927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_352075,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000363 DASVTM010000000
VERSION     DASVTM010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352075"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VF633_09875"
     CDS             complement(<1..270)
                     /locus_tag="VF633_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471410.1"
                     /translation="MPDLTYPRFEGRRVLIVEDEYVIAMEIAGILEDCGAEIVGPAGS
                     VSLAIQLIAQEAPIDAAVLDVNLGRERVFPVAETLNLRNIPFVFAT"
     gene            complement(258..1625)
                     /locus_tag="VF633_09880"
     CDS             complement(258..1625)
                     /locus_tag="VF633_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8471411.1"
                     /translation="MSEAMTPEMFEVLVDSATDFAIFTVDPVGLVTSWNRGAERVTGY
                     EASEILGQDGDVIFTEEDRAAGAPEWERETARRDGRAEDERWHRRRDGVRFWGSGAMM
                     PLRQPQLGFVKVLRDRTEQHEGQRELEASERRFRLLATHVPQLVFRSLGSGSRTWGSP
                     QWINFTGLSESQSLGTGWLDAIHPDDQEETLAAWGRASETGKYTIVHRILRASDGMFR
                     WHQTEAGPIDPADPQGSDWVGTSTDIDDVRSLQDRQAVLLAELQHRTRNLLGVAQSVS
                     RQTLRTSRSLDEFGRQFEGRLQALSRVQSLLSRTSSERVDLRSLVELEIRAHAGEANS
                     RVSIEGPQIEIPAISAQALNLAVHELATNALKYGALAHEDGRLSVSWTLEETVESHVT
                     LVWRERGVHIATPPKRKGYGSELIERALPYQLHASTSLAFDPDGVICTIRTSAYPPEP
                     SLCQT"
     gene            2242..2415
                     /locus_tag="VF633_09885"
     CDS             2242..2415
                     /locus_tag="VF633_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471412.1"
                     /translation="MQDLEARIIELIRDELGRQAEESGLMIVDLPGVVRISGDVDLEA
                     LAMAIAGALSGGP"
     gene            2412..3260
                     /locus_tag="VF633_09890"
     CDS             2412..3260
                     /locus_tag="VF633_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8471413.1"
                     /translation="MKIHHLNCGTDCPFGGHLMDGRSKGPLARLVCHCLLIETPASGL
                     VLVDTGYGMQDVSHPHPRISRTMRSALNIRLRERETALRQVEALGFSARDVRHIVLTH
                     LDFDHAGGIEDFPEARVHLMREELDAGRARRGGLASNKRYRPMQFDRVRDWRPYAQEG
                     SAWFGFGAVRNLDGLPPEVLMIPLPGHTWGHAGVAVSTQQGWLLHAGDAYFHADEMRR
                     PDRRCPPGLRAYQTLMEVDRGLRLENQARLRRLSLKTDAGVQIICAHDAAELDDCIAR
                     SGARHG"
     gene            3631..4638
                     /locus_tag="VF633_09895"
     CDS             3631..4638
                     /locus_tag="VF633_09895"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8471414.1"
                     /translation="MRLKPIADQVIVVTGASSGIGLATASLAAGRGAKVFLIARNGPA
                     LQAISDGLNAAGGQTAFAVADVGVKAQIQAAAEAAVARFGTIDSWISVAGVAVYASVL
                     DTPPEEHARMFQTNYGGVIHGAQAAVPILRQHGGAFIAVGSVASDIGTPVLGLYAASK
                     HAVKGFIDSLRIELISEGAPISVTLVKPSGIGSPLAEHAANHLGVAARVPPPAYAPEV
                     VARALLHVAEHPRREITVGGVGLLQILAASHAPRLADRISSWVPPLLRDRTRPAPPAN
                     NLEGPMSDGMISSPFEPALPFSLYTNAALNPVRALTVLATVGAVLLAVGKAVRIKSGP
                     S"
     gene            complement(4682..>5927)
                     /gene="ligD"
                     /locus_tag="VF633_09900"
     CDS             complement(4682..>5927)
                     /gene="ligD"
                     /locus_tag="VF633_09900"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX8471415.1"
                     /translation="GRDVVAKLLPRLQALETGDSPFKHKGAPRKTGDIHWVKPELVAE
                     IQYAGFTGDGSIRQASFKGLREDKPASEVEAETPAPATTQVATPTATIRVATVKPRSS
                     APVMGITISHPDKALWPDANDGVPVTKRELAEYYEAVGPWMLEHVRGRPCSMIRMPDG
                     ITGAQNFFQRHSSKGQSSLITEVEVWGDRKPYLQFDRVEALVAAAQTGALELHPWNSE
                     PFSPEQPGRLVFDLDPAPDVPFERVIEGAREVRDRLEALGLVSFCKTTGGKGLHIVTP
                     LKAKGIDWDVAKAFARDVCKAMAHDAPDRYLITMAKKDRTGRIFLDYLRNDRMATAVA
                     PLSPRGRPGAPVSMPLTWTQVKKGLDPTKYTIRSVPGLLSRSTAWEDYCDGERPLAAA
                     IERLGKVAGKRLPGSEVRAREK"
BASE COUNT         1010 a         1969 c         1889 g         1059 t
ORIGIN      
        1 cgtcgcgaac acgaagggga tgttcctcag gttcagcgtt tcggcgacag ggaagacccg
       61 ctctcggccg aggttgacgt ccagcacagc ggcgtcaatg ggcgcctcct gcgcaatgag
      121 ttggatcgcc aggctgacgg agcccgcggg cccgacgatc tctgcgccgc aatcctcgag
      181 gatcccggcg atttccatgg cgatcacata ttcgtcctcg acaatcagga cgcgtcgccc
      241 ctcgaaacga gggtaggtca ggtctggcat aatgatggtt ccggtggata ggccgaagtc
      301 cggatcgtgc agatcacccc gtcagggtcg aacgccaggc tggtgctggc atgaagctgg
      361 tagggcaagg cacgctcgat cagttcacta ccgtaaccct tgcgcttcgg cggcgtggcg
      421 atgtgaacac cgcgctcgcg ccagaccagc gtcacgtgcg actcgacggt ctcctcaagc
      481 gtccaggaga ccgacaagcg tccgtcttcg tgtgcaaggg cgccgtactt gagggcgttg
      541 gtcgctagct catgcaccgc cagattcaac gcttgcgccg agatggccgg gatttcgatc
      601 tgcggtccct cgatagacac gcggctgttc gcctcgcccg cgtgggcccg gatttcgagt
      661 tcgaccagac tacgcaggtc cacgcgctca ctgctcgttc tggagagaag gctctgcaca
      721 cgcgacaatg cctgcaagcg gccttcaaac tgcctgccga attcgtccag ggaccgagaa
      781 gtgcgcaagg tctggcgact gacggactgc gcgacgccca gcagattccg cgtccgatgt
      841 tgcaactcgg ccaggagcac ggcctgccgg tcctgcaggc tgcgcacgtc gtcgatgtcg
      901 gtcgatgtcc ccacccagtc cgacccttgc ggatcagccg gatcgatcgg cccggcttcg
      961 gtctgatgcc atcggaacat gccgtcgctc gcccgaagga tgcgatggac gatagtgtat
     1021 tttccggttt ccgacgccct cccccacgcc gccagtgtct cttcctgatc atccgggtgg
     1081 atggcgtcca gccagccggt cccgaggctc tgcgactcgg acaatccagt gaagttgatc
     1141 cattgtggac tgccccaggt tcggcttccc gatcccaacg accgaaacac cagctggggc
     1201 acgtgagtgg cgagaaggcg gaagcgtcgc tcgctggcct ccagctcacg ttgaccctcg
     1261 tgttgctccg ttcggtcccg caacaccttg acgaagccaa gctgcggctg tcgcaggggc
     1321 atcatggccc ccgaccccca gaacctcacc ccgtcccggc gacgatgcca gcgctcatcc
     1381 tcggcccgcc cgtcgcgtcg ggcggtctcg cgctcccatt ccggtgcgcc cgccgcccga
     1441 tcctcctccg tgaagatgac gtctccgtcc tgcccaagga tctcggacgc ttcgtagccg
     1501 gtgacgcgct cggcgccccg gttccagctg gtgacaagcc caaccggatc aacggtaaag
     1561 atggcgaagt ccgtcgcgct gtccacgaga acctcgaaca tttccggggt catagcctcg
     1621 ctcatggcga cacccagccc tgaggcacga ctccggtatc gcgttttcgg agggcgatgg
     1681 acttcctccg catggtgacg gtgggcgcga acgcgatagc cgcaagcgtc agtgaactga
     1741 ggacatgcaa cccgggcgcg actgctcccc gccggtagcg agacctggct agcagcgcca
     1801 ggtcgccgat ggggatgaga gaggccagac cggctgtgac ccgcagactt gcctcccctc
     1861 cccgggatgc ctggatcagc cccgctgcca gaacaatatc ccggatcccg agcgcccgaa
     1921 catagtcgag gtcaggctca cgagatcgga taccgaacaa ccgggtcgcc tgacggggct
     1981 tcgaaaggaa gacgcagccg agaccaaaca gcccgactcc cgtcacccgc gcccccaacg
     2041 gacgtgcgcc gcgagcagcc ggcgggttga gcttgatcgg cattaaggga ctcgctgcgt
     2101 cgaaggatat cgggatcaac tgcttcggtg gtgtatcgtt cgccgatgag gtcgccgtgt
     2161 cgtcgatcag acaggctggc ccgcttcgcc cgactgcgcg tttgtcgaca gcgccggaaa
     2221 cccggctgac gcggagccgg catgcaggat ctggaagcca gaatcatcga actcatccgc
     2281 gacgagctcg gccggcaggc cgaagaatcc ggtctgatga ttgtcgacct ccctggtgtc
     2341 gttcgcatca gcggcgacgt cgatctggag gccctcgcga tggcgatcgc cggcgcactc
     2401 tcgggggggc catgaaaatc caccatctga actgcggaac cgactgccct ttcggcggcc
     2461 atctgatgga cggtcgcagc aagggccccc tcgcccgtct cgtgtgccac tgtctgctaa
     2521 tcgaaacgcc ggcctcgggg ctggttctgg tcgataccgg ctacgggatg caggacgtta
     2581 gccatcccca tccgcgcatc agtcgcacga tgcgctcagc cctgaacatc cggctccgcg
     2641 aacgggagac cgccctccgg caggttgagg cgctgggctt ctcggccagg gacgtgcgcc
     2701 acatcgtcct cactcatctc gatttcgatc acgctggagg aatcgaggac tttccggagg
     2761 cgcgcgttca tctgatgcgc gaagaactcg atgccggacg ggccaggagg ggcggacttg
     2821 cctcaaacaa gcgctaccgc cccatgcagt tcgaccgggt gcgggactgg cgaccgtacg
     2881 cacaggaagg cagcgcctgg ttcggcttcg gcgccgtccg caacctggat ggattgccgc
     2941 ccgaggtatt gatgatcccc ttgcccggcc atacctgggg ccacgccggc gtcgccgtgt
     3001 cgacgcagca aggatggctc ctccacgccg gcgacgccta tttccacgcg gacgagatgc
     3061 gtcgaccgga ccggcgatgc ccccccggcc ttcgcgccta tcagaccctc atggaagtgg
     3121 atcgtggcct gcggttggag aaccaggcgc ggcttcggcg cctgtcattg aagactgatg
     3181 caggggtgca gatcatctgc gcacacgacg ccgccgaact ggatgattgc atcgcgcgct
     3241 cgggggcgcg acacggctag gacatccgtt cggaactccc gcagccgaca aggcctggcc
     3301 gctggtccgc aaagatgtaa tgggcttcgc ttgctctatc atccttgccg acccgcgggc
     3361 ggtcgacgcc gttctccgtg tcgcctgcca gcgatggatt gaaccaaatg gcgacgcccg
     3421 cggttggctc ctgaacaagg agatgcagat gcacgacgcg agcttgagtc cgacccggag
     3481 ggtgtccggg gtcggggcgg aaactgttgt gccctttgcg cggcacgccc gcgacgccac
     3541 cgctgcactg attgacgacc gcctgttggt ggagggcggc ctgcacgggc gcatgggccg
     3601 gaaacacgct ttcagtggag cgttgccggc atgcgtctga aacccattgc cgatcaggtc
     3661 atagtggtta ccggcgccag ctcggggatc ggactggcga cggcgagcct cgccgccggc
     3721 cggggcgcaa aagtcttcct gatcgccaga aacggtccgg cgctgcaggc gatcagcgat
     3781 ggactgaatg cagccggggg ccagactgca ttcgccgtcg ctgatgttgg cgtgaaggcg
     3841 cagatccagg cggcggccga ggcagcggtg gcgcggtttg gaaccatcga cagctggatc
     3901 agcgtcgccg gcgtggcggt ctatgcaagc gtcctcgata cgcctcctga ggagcatgcc
     3961 cggatgttcc agaccaacta tggcggcgtc attcacggcg cccaggcggc tgtgccgatc
     4021 ctcagacagc acggcggcgc attcatcgct gtcggctcgg tggcgtccga catcggtacg
     4081 cccgtgctcg gcctctacgc tgcgtccaag cacgcggtga agggtttcat cgactccctg
     4141 aggatcgagc tgatctccga gggcgcgccg ataagcgtga cgctggttaa gcccagcggc
     4201 ataggctccc cgcttgcgga acacgccgcc aaccacctgg gcgtagccgc cagggtgccg
     4261 ccgcccgcct atgcgcccga ggtggtcgcc cgcgcgctgc tgcatgtggc agaacatccc
     4321 cgccgagaaa tcaccgtagg cggagtcggt ctgctacaga tcctcgcagc gtctcatgcg
     4381 ccgcgtttgg ccgaccggat ttcctcctgg gtcccgcccc tgttgcgtga ccggacacgg
     4441 cctgctcctc cggccaacaa cctcgaaggg ccgatgtccg acgggatgat ttcgtcgccg
     4501 ttcgaaccgg cgctgccgtt cagcctttac accaacgctg cgttgaaccc cgtgcgggcc
     4561 ctcaccgtcc tcgcaactgt cggtgcggtg ctgttggcag tcggcaaggc cgtccggatc
     4621 aagtcaggtc ccagctaacg atcatccctg ctcgaataat ggagcgatcc tgtcggaggg
     4681 gtcatttctc tcgcgcccga acttccgacc ccggcagccg ctttccggcg acctttccga
     4741 gccgctcgat cgctgcggca agcggccgct cgccgtcaca atagtcctcc caggcagtgg
     4801 accgcgagag caatccgggc acgctgcgta tcgtgtactt ggtgggatcg aggcctttct
     4861 tcacctgcgt ccaggtcaaa ggcatggaga ccggcgcgcc cggccgcccg cgaggggaca
     4921 agggcgcgac cgccgtcgcc atgcggtcgt tacgcagata gtccaggaaa atccttcccg
     4981 tacgatcctt cttggccatg gtgatgaggt atcggtcggg cgcatcgtgg gccatggcct
     5041 tgcaaacgtc gcgggcaaac gccttggcga catcccagtc gatgcccttg gctttcaggg
     5101 gggtgacgat atgcagcccc ttgccgccgg tggtcttgca gaagctgacc agcccgaggg
     5161 cttcgagccg gtcccggacc tccctggcgc cctcaatcac gcgttcgaac gggacgtcag
     5221 gcgcgggatc caggtcgaac accagccggc cgggctgctc gggcgaaaag ggctcgctgt
     5281 tccacggatg aagttccagg gctcccgtct gggcggcggc gaccagagcc tcgacccggt
     5341 cgaactggag atacggtttg cgatctcccc agacctcgac ctccgtgatc aacgacgact
     5401 ggcccttcga agagtgccgc tggaaaaaat tctgtgcgcc tgtgatcccg tccggcatgc
     5461 ggatcatcga acagggccgt cccctgacat gctccagcat ccatgggccg accgcctcat
     5521 agtactctgc aagttcgcgt tttgtcaccg gcacgccgtc gttcgcatcc ggccataagg
     5581 ccttgtcggg atgggagatg gttatgccca ttacaggcgc tgagctgcgc ggcttgacgg
     5641 tcgcgaccct gatcgtcgca gtgggcgtgg cgacctgagt ggtcgcagga gccggggtct
     5701 ccgcctcgac ttcgctggcc ggcttgtctt cccgtaatcc cttgaacgac gcctgccgga
     5761 tcgatccgtc tccggtgaag ccggcgtact ggatttcggc caccagttcc ggcttcaccc
     5821 aatggatatc accggtcttg cgaggtgccc ccttgtgctt gaaagggctg tccccggttt
     5881 ccagggcctg gagacgaggc agaagctttg cgacgacgtc gcgcccg
//
LOCUS       DASVTM010000364         6296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_303018,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000364 DASVTM010000000
VERSION     DASVTM010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303018"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..2986)
                     /gene="clpB"
                     /locus_tag="VF633_09905"
     CDS             complement(398..2986)
                     /gene="clpB"
                     /locus_tag="VF633_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX8471416.1"
                     /translation="MNLDLYSDRAKQAVQSAQSLALARRHQQFAPEHLLKVLLEERDG
                     LARNLITTAGGDAAKAETATETALQKRAQVSGGSGQLYLDGDTARVFATAEDASKKSG
                     DAFVTTERLLSAIAREGGTAKEILKSVGATPDKLDAAITEIRKGKTADSAAAEDGYDA
                     LKRYARDLTQAARDQKIDPVIGRDEEIRRTIQVLARRTKNNPVLIGEPGVGKTAIVEG
                     LALRIVNGDVPESLREKKVMSLDMGSLIAGAKYRGEFEERLKAVLNEVGAAEGQIILF
                     IDEMHTLVGAGKGDGAMDASNLLKPALARGELHCVGATTLDEYRKHVEKDAALARRFQ
                     PVFVAEPTVEDTVSILRGLKEKYEVHHGVRISDSAIVAAATLSNRYITDRFLPDKAID
                     LIDEAASRVRMAVDSKPESLDEIDRRLVQLKIEREALKKETDSASIQRLDKLEDDISD
                     LTIESDDLTRRWKAEKEKVGQGAQLRETLDRLRADLALAQRQGDLGRASEIAYGQIPT
                     IEKQLAEAEKNETSGGGPLTPEVVDAEQIAQVVSRWTGVPVDKMLEGEREKLLSMEDS
                     LRGRVVGQDEALEAVADAVRRARAGLNDPNRPLGSFLFLGPTGVGKTELTKALAAFLF
                     DDDAAITRLDMSEYMEKHSVSRLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEVEK
                     AHPDVFNVLLQVLDDGRLTDGQGRTIDFRNTLIIMTSNLGSEALAGQHEGDDVEAVRP
                     YVMESVRAHFRPEFLNRIDEIILFRRLGRDQMSGIVRIQLARLEKLMADRSLTLALDD
                     SAAAWLADKGYDAVYGARPLKRVIQKDLVDPIAKKLLAGELEDGSVIAVSAGDGKLEI
                     GKARVH"
     gene            3247..3666
                     /locus_tag="VF633_09910"
     CDS             3247..3666
                     /locus_tag="VF633_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEX8471417.1"
                     /translation="MRKLLIAAAAGLMTLSALPATAGAAQERPYNAGPMSGQNHGGQN
                     HGGSNQNGPGHNNSGPNRPGQGRPDQAGPNRPGQSSQYGNWDDRWGARPSAPPRGWGR
                     NNDWYRHVRACSQRYRSYNPRTDTFVVRRGVAARCRL"
     gene            complement(3725..4837)
                     /gene="pelA"
                     /locus_tag="VF633_09915"
     CDS             complement(3725..4837)
                     /gene="pelA"
                     /locus_tag="VF633_09915"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX8471418.1"
                     /translation="MIHSDTIRGRAFAAILGLSALSVAACAPALAQTPATAPGTVAWG
                     SAVLRQPAAWYFTPEARRLADNVLLYQSANGSWPKNTDMSAPPVGPLDPHLADTIDNQ
                     ATTLEMGFLARVIQAGGAPSQPAYVAAFNKGFDWLLAAQYPNGGWPQFFPLRHGYYDH
                     ITYNDDAMVRVLNMFRDVADGKAPYGLVDADRKAKAAGSVARGVDVILKTQIHQNGQL
                     TVWCAQHDEVTLAPTWARKFEPPSLSGYESVGITRFLMGVPNPSPEVIAAIEGSVAWF
                     KTTVIPDIAVERFTNAEGLADRRVIPAPGARLWARFYELDTNRPIFLDRTSVFQPALA
                     DLDRERRAGYNYYDGAAAALIDTDYPAWRARVGPAR"
     gene            5020..5730
                     /locus_tag="VF633_09920"
     CDS             5020..5730
                     /locus_tag="VF633_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471419.1"
                     /translation="MIELLASLIHAPAQGFQLDGSLIRDDQSCYTITMTRDGASTPVG
                     VTLQTIDRVQLQGRPALRIVVHQSVNSGAFDMRDTFTLDAATLAPIRLENDRKGQRHV
                     TLDYAADRVIGSRLEDGVSKPVDVALPGTVWEGNLFGVTFAALPLAAGATFSIPFYQY
                     DKGVGAFAVRVAGEEVVTTPQGPVDAWTLDVSTGAGPGMTYLIAKADRRELGYRTPRG
                     GQTLGGDCSALIEASSGG"
     gene            5749..>6296
                     /locus_tag="VF633_09925"
     CDS             5749..>6296
                     /locus_tag="VF633_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX8471420.1"
                     /translation="MTTIAILTPDPADSSYAGQWPQVLARLEATLALADIVAVPESWT
                     DYIEDASRLTRFPLVLPVLLWGYHRDHERFLQAVRTWTKAGVPLANPAVVLGWNSDKT
                     YLRRLEAAGVPIPPTRWTDKVTDADVEAAFEAFGVDELVIKPTVSGGAWKTLRLTRGQ
                     PLIDPPAGSAMIQPYLPAIATEG"
BASE COUNT         1034 a         1951 c         2178 g         1133 t
ORIGIN      
        1 cgtctaccat ggcaatgatt tcgtcgcggg cattctcatc tatctcgcca ccagtgaacc
       61 actggatcga aagcgccttc aaagtcgatt tctgctcgat tatttttggc tcatgtttgt
      121 ccgacttatc cactgctttc cttagctgcc agtaaatgga acacggatcc gaagatggcg
      181 gctgtaaggc ccctgcagcg taacgatgca tggttgccga actgaaggtg ggactgcacc
      241 agacgtaatg tttatcgtgc cggaaatcgc gctgaatgcg gaacttcaaa tgagtgtttg
      301 tcgaccataa aagctcagcc aagcacttcc ccacgcaacg ttaagtagct acctacccgg
      361 cgtttcaacg ttctgcaaga tttctagccg tgctttttta atgcacccgc gccttcccga
      421 tctccagctt cccgtccccc gctgacacgg cgatcaccga cccgtcctcc agctcaccgg
      481 ccaggagttt cttggcgatc gggtcgacca gatccttctg gatgacgcgc ttcaaaggcc
      541 gcgctccgta caccgcatcg tagcccttgt cggcgagcca ggcggcggcg ctgtcgtcca
      601 gcgccaaggt caggctgcgg tcggccatga gcttttctag gcgggcgagc tggatgcgga
      661 cgatgccgga catctggtcg cggccgaggc ggcggaacag gatgatttcg tcgatgcggt
      721 tgaggaactc cgggcggaaa tgggcgcgga cgctttccat gacgtagggg cggacggcct
      781 cgacgtcgtc accctcatgc tgacccgcga gcgcctcgga ccccaggttg gaggtcatga
      841 tgatcagggt gttgcggaag tcgatggtgc ggccttgccc gtcggtcagc cgaccgtcgt
      901 cgaggacctg cagcaggacg ttgaagacgt cggggtgggc cttttcgacc tcgtcgaaca
      961 gaacgacctg ataggggcga cggcgcacgg cctcggtcag ggccccgccc tcgtcatagc
     1021 cgacatagcc gggaggggcg ccgatgaggc ggctgacgct gtgtttctcc atgtattcgg
     1081 acatgtcgag gcgggtgatg gccgcgtcgt cgtcgaacag gaaggcggcc agcgccttgg
     1141 tcagctcggt cttgccgacg ccggtggggc cgaggaacag gaaggagccg agcgggcggt
     1201 tggggtcgtt cagcccggcg cgggcgcggc gaacggcgtc ggcgacggct tccagcgcct
     1261 catcctgacc gaccacgcgg ccgcgcaggg agtcttccat cgacagcagt ttttcgcgct
     1321 cgccctccag catcttgtcg accggaacgc cggtccagcg tgagacgacc tgggcgatct
     1381 gttcggcgtc gacgacctcg ggggtcaggg ggccgcctcc cgaggtttcg ttcttctcgg
     1441 cctcggcgag ttgtttctcg atcgtgggga tctggccgta ggctatttcc gacgcgcggc
     1501 cgaggtcgcc ctgacgctgg gccagcgcaa ggtcggcgcg caggcggtcg agggtctcgc
     1561 gcagctgggc gccctgaccg accttttcct tctcggcctt ccagcggcgg gtcaggtcgt
     1621 cggactctat ggtcaggtcc gagatatcgt cctcaagctt gtccagacgc tggatcgagg
     1681 cggagtcggt ttccttcttc agggcctcac gctcgatctt gagctggacc agacgacggt
     1741 cgatctcgtc gagggattca ggcttggaat cgacggccat gcgaacgcgg ctggcggcct
     1801 catcgatcag gtcgatggcc ttgtcgggca ggaagcggtc ggtgatgtag cggttcgaca
     1861 gggtggcggc ggccacgatg gcgctgtcgg aaatgcgcac gccgtggtgg acctcgtact
     1921 tctccttgag gccgcgcagg atcgaaacgg tgtcctcgac cgtaggctcg gccacgaaca
     1981 ccggctggaa gcgacgggcg agggcggcgt ccttctcgac gtgcttgcgg tactcatcca
     2041 acgtggtggc gccgacgcag tgcagctcgc cgcgcgcgag ggcgggcttg agcaggttgg
     2101 aggcgtccat ggccccgtcg ccctttccgg cgccgaccag ggtgtgcatc tcgtcgatga
     2161 acaggatgat ctggccctcg gcggcgccga cctcgttgag cacggctttc agccgctcct
     2221 cgaactcgcc acggtatttc gcgccggcga tcaacgagcc catgtcgagc gacatgacct
     2281 tcttttcgcg cagggattca ggcacgtcgc cgttgacgat gcgcagggcc agaccctcga
     2341 cgatggcggt tttgccgacg ccgggctcgc cgatcaggac ggggttgttc ttggtgcggc
     2401 gggcgaggac ctggatggtg cggcggatct cctcgtcacg gccgatgacg gggtcgatct
     2461 tctggtcgcg ggcggcctgg gtcaggtcgc gggcatagcg tttgagggcg tcatagccgt
     2521 cctcggcggc ggcgctgtcg gccgtcttgc ccttgcggat ttcggtgatg gcggcgtcca
     2581 gcttgtcggg cgtggcgccg acggacttca ggatttcctt cgctgtaccg ccttcgcggg
     2641 cgatggcgct gagcaggcgt tcggtggtga cgaaggcgtc gccggatttc ttggacgcgt
     2701 cctcggcggt ggcgaagacg cgggcggtgt cgccgtcgag atagagctgg cctgagccgc
     2761 cggagacctg ggcgcgcttc tgcaacgcgg tctcggtcgc ggtctcggcc ttggccgcat
     2821 cgccgccggc ggtggtgatc aggttgcggg cgaggccgtc gcgttcctcc agcagcacct
     2881 tcagcagatg ctcgggggcg aactgctggt gacggcgcgc gagggcgagc gactgggccg
     2941 actggacggc ctgtttggcg cggtcggagt agaggtcgag gttcaaggcg tatcccctgt
     3001 agaggcggaa gtcgatttgc ttggccgaga gcgccaagcg gtggcttgaa accgtccgac
     3061 gcgtcctctg tagcaagcaa cgcgccgttg ggctgagcca gatttgggtg cgggcgggca
     3121 gaggtacaag ggcggcgagc gagcattgaa atctcgcctg cggtgcaacg tcgcatgcgg
     3181 cagggggttt ggactggaca gccgagggcc tcgtcggggc ccgggcctca cagagaaaga
     3241 tactcgatga gaaagctgct gatcgccgcc gccgccggtc tgatgaccct gagcgcactt
     3301 cctgcaacag ccggcgctgc gcaggagcgg ccctacaacg ccggacccat gagcggccag
     3361 aaccatggcg gccagaatca tgggggctcg aaccagaacg gtccgggtca caacaactcg
     3421 gggcccaatc gtccggggca aggccgaccc gaccaggcgg gaccaaaccg tccgggacag
     3481 agcagccaat acggcaactg ggacgaccgt tggggcgcgc gtccctcggc gccgccgcgc
     3541 ggctggggcc ggaacaacga ctggtaccgc cacgtccgcg cctgctcgca gcgctaccgc
     3601 agctacaacc cgcgcaccga caccttcgtc gtgcgccgtg gcgtcgccgc ccgctgccgc
     3661 ctgtaggctg aagccaaccg actgaagacc gcccgccggg gagacctggc gggcgttttc
     3721 gtgcctagcg ggctgggccg acccgtgcgc gccacgccgg atagtccgtg tcgatcaggg
     3781 cggcggcggc tccgtcatag tagttgtacc cggcgcggcg ctcgcgatcg agatcggcca
     3841 aggctggctg gaacaccgag gtgcggtcga ggaagatcgg gcggttggtg tcgagctcat
     3901 agaaccgggc ccacaggcgg gcgccggggg ccgggatcac gcggcggtcg gcgaggccct
     3961 cggcgttggt aaagcgttcg acggcgatgt ctggaatgac ggtggtcttg aaccacgcga
     4021 ccgagccctc gatggcggcg atgacctcgg gcgaggggtt ggggacgccc atcaggaaac
     4081 gggtgatacc gacgctctca tagcccgaca gcgacggcgg ctcgaatttg cgcgcccagg
     4141 tcggggcgag ggtgacctcg tcatgctggg cgcaccagac ggtcagctgc ccgttctggt
     4201 ggatctgggt cttgaggatg acatcgacgc cccgggcaac cgacccggcg gccttggcct
     4261 tgcggtccgc atcgactaaa ccgtagggag ccttgccgtc cgcaacgtcg cggaacatgt
     4321 tcagcacccg caccatggcg tcgtcgttgt aggtgatgtg gtcgtagtag ccgtgacgca
     4381 gcgggaagaa ctggggccag ccgccgttcg gatattgcgc cgccagaagc cagtcgaagc
     4441 ccttattgaa ggcggcgaca tacgctggct ggctgggagc tccaccggcc tgaattacgc
     4501 gggccagaaa gcccatctcc agtgtggtgg cctgattgtc gatcgtgtcg gcgaggtgtg
     4561 ggtcaagcgg gccgaccgga ggggcggaca tgtcggtgtt cttgggccac gacccgttgg
     4621 ccgattgata cagcagcaca ttgtcggcca gacggcgagc ctcaggggtg aagtaccagg
     4681 cggcgggctg acgcaggaca gcgctacccc aggcgaccgt accgggcgcg gttgcgggtg
     4741 tctgggcgag ggccggagcg catgccgcaa cagacagggc cgacaagcca aggatggctg
     4801 caaatgcgcg accacgaatg gtgtctgaat ggatcatggt cttcctcccg atggaccttt
     4861 gcggcccgtc tcgtccgtcc ccgacggcga agtctgcgat ctgcatacat cagaccggcg
     4921 ctacccgaaa gacaccggtg tcagaaatgg gcgcatggac gtttgttcac ttgctgcggc
     4981 aatgtccaga tgtgaccctg agaaacaccg gagcctgaca tgatcgaact tctcgcgtcg
     5041 ctgatccatg cgcccgctca gggttttcag ttggatggct ccctgatccg tgatgatcag
     5101 tcctgctaca cgatcaccat gacgcgggac ggggcatcga caccggtggg cgtgaccttg
     5161 cagaccatcg atcgtgtcca actccagggc aggcccgccc tgcgcatcgt cgtccaccag
     5221 agcgtcaaca gtggcgcctt cgacatgcgc gacaccttca cgcttgacgc agccacgctg
     5281 gcgccgatcc ggctggagaa cgaccgcaag gggcaaagac acgtgaccct cgactatgcc
     5341 gccgatcgcg tcatcgggag ccgtctggag gacggtgtgt caaagcccgt cgatgtcgcg
     5401 ctgcccggaa cggtctggga aggcaatttg tttggcgtga ccttcgccgc cctgccgctg
     5461 gcggcaggcg cgacattcag catccccttc tatcaatacg acaagggcgt cggcgcgttc
     5521 gcggttcggg tagcgggcga agaggtcgtg acgacgccgc agggtccagt cgacgcctgg
     5581 acgctcgacg tgtcgaccgg agcgggaccg ggcatgacat atctgatcgc aaaggccgac
     5641 cgtcgcgagt tgggttaccg aacgcccagg ggcggccaga cgctgggcgg agactgttcg
     5701 gcattgatag aggcttcctc cggcggctga aatcggcttt gaggcggtat gaccaccatc
     5761 gccatcctca cgcccgaccc cgctgattcc tcctacgccg gccagtggcc tcaggtgctg
     5821 gcgcgtttgg aggccacact cgcgctggcc gatatcgtcg ccgtgcccga aagctggacc
     5881 gattatatcg aggacgccag ccgcctgacg cggttcccgc tggtcctgcc ggtgctgttg
     5941 tggggctatc accgcgatca cgagcgcttc cttcaggccg tcaggacctg gaccaaggcg
     6001 ggcgtgcctc tggccaatcc ggccgtggtg ctgggttgga actccgacaa gacctacctg
     6061 cgccgtcttg aagcagcggg tgtgccgatc ccgcccacgc gctggaccga caaggtcacc
     6121 gacgccgatg tcgaggctgc gttcgaggcg tttggcgtcg atgagctggt gatcaagccg
     6181 accgtctcgg gcggggcgtg gaagacgttg cgcctgacgc gcggccaacc cctgatcgat
     6241 ccgccggccg gctcggcgat gatccagccc tacctccccg ccatcgcaac cgaggg
//
LOCUS       DASVTM010000365         6874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_403991,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000365 DASVTM010000000
VERSION     DASVTM010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403991"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VF633_09930"
     CDS             <1..280
                     /locus_tag="VF633_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX8471421.1"
                     /translation="AIPDTLARKVDLWKARGQFIRYRWEMFHPASWLAIYDGFGVMPG
                     QLDPAVEGLDPSQLERSLRQMKAAVADVVARTPSHAAFLARLGRPEAA"
     gene            292..1821
                     /locus_tag="VF633_09935"
     CDS             292..1821
                     /locus_tag="VF633_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8471422.1"
                     /translation="MQTDPNRPIRKICVVGGGTAGWMAAALMSEHFKGTMGEIEVVES
                     DDIGIIGVGESTVPPLLQLLAKLGIDEQVFVRETQATFKLGIDFRDWRVKGERYFHPF
                     GDIGAKVDMADFYQLWLKARANGYQGALQDFAPAAVMAREGKFTLPFKAPRTPIASAN
                     YALHFDARRVARFLRTYAETRGVKRTEGMVEDVALHPNGFVEKLILKDGRTVEADFFI
                     DCSGFRSLLIGKALQVGYQDWSHWLLCDRAVAVQTENVSPPMPYTMVQAQDFGWRWRI
                     PLQHRTGNGYVFSSKHLSDDEATATLMNQVEGAPVVKPMVIPFKTGVRDRLWDKNVLA
                     LGLAGGFIEPLESTAIHLIYRGMDFFFRLLPDRDCDPALAAEYNRRMITDYEEIRDFI
                     ILHYCTTSRDDTAFWRECRDMEPPPSLKRKIELFRVNGSLAEGLDELFRAVSWQAVME
                     GMGVHPRGYHPLVDRIPFDGVPAQLDHAAELLTRGVADLPSQEEFIARHCAAARPGIA
                     A"
     gene            1901..2941
                     /locus_tag="VF633_09940"
     CDS             1901..2941
                     /locus_tag="VF633_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8471423.1"
                     /translation="MRRPVTIKHVAAEAAVSLQTVSRVINNGPNVRPEVRDRVNAAVA
                     RLGYVPSLAAQRMGGSRSWLLLALNDRDRTIEGWRLGEGTDWVDQMLMGGMLKCAEHG
                     YRMIFELVDTHSSHVEREVRAAISALHPDGVILTPPHSDNPLIIDLLVQLGLPFARIG
                     SVAEGPGFAITMDDRAAGRAATDHLLDLGHTRVGFITGSDEWAISGARLDGYKQAILG
                     RGLELDEALIGRGDFSFESGTAAMEAFYELAAPPTALVASNDQMALAALRVANGRGMK
                     SPDHLALISFDDTPIVKFSNPPLTAIVQPIAVMAAEAARLLIQAKAGEADLPTSVVVP
                     FSLAIRDSTRRA"
     gene            2996..4291
                     /gene="hmgA"
                     /locus_tag="VF633_09945"
     CDS             2996..4291
                     /gene="hmgA"
                     /locus_tag="VF633_09945"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX8471424.1"
                     /translation="MSTSARHYLPGFGNHGVSEAVPGALPQGQNSPQRAPFGLYAEQL
                     SGTAFTAPRHENRRSWLYRLRPTANHPPFTPWTSATLLKSGPFERLPTPNRLRWDPLA
                     MPSAPTDFVEGLVTYGGNGDVGQTAGIGIHLYACNRSMENRVFFNADGELLIVPQEGG
                     LVITTEFGIIDLEPQQIAVIPRGVRFRVALTSQQARGYVCENYGALFRLPDLGPIGSN
                     GLANPRDFETPVAAYEDLDGPVELIQKFQGELWSTTLDHSPFDVVAWHGNLAPYRYDL
                     RRFNTINTVSYDHPDPSIFTVLTSPSDTPGTANCDFVIFPPRWMVAEDTFRPPWFHRN
                     VMSEFMGLILGAYDAKEGGFAPGGASLHNCMSGHGPDQASYAKAVEAELKPHKIENTM
                     AFMFESRWVIRPTDHAMDSPTMQLDYDDVWSDFDKGQPV"
     gene            complement(4315..4836)
                     /gene="msrA"
                     /locus_tag="VF633_09950"
     CDS             complement(4315..4836)
                     /gene="msrA"
                     /locus_tag="VF633_09950"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8471425.1"
                     /translation="MAKTETAILAGGCFWGVQDLLRRYPGVIETRVGYSGGDVPNATY
                     RNHGTHAEAIEIIYDPELITYRRLLEFFFQIHDPSTLNRQGNDLGLSYRSAIFYTSDE
                     QKQVALDTIADVDASGIWPGKVVTEVTAAGPFWEAEPEHQDYLERLPDGYTCHFIRPN
                     WTLPVRAEAEAAE"
     gene            5064..5153
                     /locus_tag="VF633_09955"
     tRNA            5064..5153
                     /locus_tag="VF633_09955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5098..5100,aa:Ser,seq:gga)
     gene            complement(5686..>6874)
                     /locus_tag="VF633_09960"
     CDS             complement(5686..>6874)
                     /locus_tag="VF633_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471426.1"
                     /translation="DALVYLIDENRFPSGEWRSRDTVVKSMTELSDNVVERGASHRDA
                     DVRAMAVRVLATRKPLAPELSSDLRTDPSAKVRMAAVEVALQAGESLSLGDIAKIVVQ
                     TETNAFLPKRKVGEEEYSKLREIVFKAMPDDDLESELDSFFTPANVAYSTLLLRHWKQ
                     RSKTARANLTDSFVSYFDTIKKSYLARFGETRAESMIPNEGLMEFVASNMIKSTLEVF
                     AKKGDASDIRFFRSAVSSKYYIIQPSALAFFGKHGEWSDIMLIASATRSTGMGLFSDS
                     TVSNDELAVTIRKIAGQRLLECIQLDIDFGIKSAIIKNYPQNAFRSLSNEAIISLLNE
                     VNDEMRKWSAVKVCECLSRDRVSVILRQYMDQSYLYYNVIHWLDLKVSAPAHITRLVA
                     SRQ"
BASE COUNT         1276 a         2108 c         2162 g         1328 t
ORIGIN      
        1 cgccattcct gacaccctgg ctcgcaaggt cgatctctgg aaggcgaggg gacagttcat
       61 ccgctatcgc tgggaaatgt tccatccggc cagctggctg gcgatctacg acggcttcgg
      121 tgtcatgccc ggccaactcg accccgcagt cgaggggctt gacccaagcc agctcgagcg
      181 cagcctgcga cagatgaagg cggccgttgc cgatgtcgtc gccagaaccc cgagccatgc
      241 ggcctttctg gcccggctcg gacgacccga agctgcctga gagatcacct gatgcaaacc
      301 gaccccaatc gcccaatccg caagatctgc gtcgtcggag gcgggaccgc cggctggatg
      361 gccgccgcct tgatgtctga gcacttcaag gggacgatgg gcgagatcga ggtcgtcgag
      421 tccgacgata tcgggatcat tggggttggc gagtcgaccg tgccgccctt gctacaactc
      481 ctcgccaaac tgggcatcga cgaacaggtc ttcgtccgcg agacccaggc cactttcaag
      541 ctgggaatcg acttccgcga ctggcgggta aagggcgagc gctacttcca tccgttcggc
      601 gacatcggag cgaaggtcga tatggccgac ttctatcagc tgtggctgaa ggcgagggcc
      661 aatggatatc agggggcctt gcaggacttc gcgccggcgg cggtgatggc gcgagagggg
      721 aaattcaccc tgccgttcaa ggccccacgc acaccgatcg caagcgccaa ctatgcgctt
      781 cacttcgacg ccaggcgggt tgcccgcttc ctgcgcacct atgcggaaac gcggggcgtc
      841 aaacggacgg agggcatggt cgaggatgtc gccctgcatc cgaacggttt cgtcgaaaag
      901 ctgatcctca aggacggtcg cacggtcgag gccgacttct tcatcgactg ctcgggcttc
      961 cgcagcttgt tgatcggcaa ggccctgcag gtcggctacc aggactggtc acactggctg
     1021 ctgtgtgatc gggctgttgc cgtgcagact gagaacgtca gcccgccgat gccctacacg
     1081 atggtgcagg cccaggattt cggctggcgc tggcgtatcc cgctgcaaca tcgcacaggc
     1141 aacggctatg tgttctccag caagcacctg agcgacgacg aggcgaccgc gacgctgatg
     1201 aaccaggtcg agggcgcgcc cgtggtcaag ccgatggtta ttcccttcaa gacgggcgtg
     1261 cgtgaccggc tctgggacaa gaatgtcctg gcgctgggtc tcgcgggcgg cttcatcgag
     1321 ccgctggagt cgacagcaat ccacctgatt tatcgcggga tggatttctt ctttcgacta
     1381 ctgcccgatc gtgactgcga tccggcgctg gccgctgaat acaatcgacg catgatcacc
     1441 gactacgagg agatccggga cttcatcatc cttcactact gcacgaccag cagggacgac
     1501 acggcgttct ggcgtgaatg ccgcgacatg gagcccccgc cgtcgctcaa gcgcaagatc
     1561 gagctgtttc gcgtcaacgg cagtctggcc gagggcctgg acgagctttt ccgggctgtc
     1621 agctggcagg cggtgatgga gggtatgggc gtgcatccgc gcggctatca tccgctggtc
     1681 gatcgcattc cgttcgacgg tgtgcccgcg cagctggatc acgcagccga actgctcaca
     1741 cgtggcgtcg cggacctgcc cagccaggag gagttcatcg cccggcattg cgcggcagcc
     1801 aggcccggga tagcggcctg atcggatgag acgccgggga cgggatcggc tagcgtgacc
     1861 ttatgactga tttgtgcgga aagacgggga tcggatcagc gtgcgtcgcc cggtaacgat
     1921 caagcatgtc gccgccgagg ccgccgtttc gctgcagacc gtgtcgcggg tcatcaacaa
     1981 cgggcccaac gtgcgccctg aggtgcggga ccgggtcaat gccgccgtcg cccgtctggg
     2041 ctatgtgccc agtctggcgg cccagcgcat gggcggttca cggtcgtggc tgcttctggc
     2101 gttgaacgac cgggaccgga cgatcgaggg ctggcgcctg ggcgagggca ccgactgggt
     2161 ggatcagatg ctcatgggcg gcatgctcaa atgtgcagag cacggctacc ggatgatctt
     2221 cgaacttgtc gatacccatt cgtcccatgt tgagcgcgag gtccgggcgg cgatctccgc
     2281 cctgcacccc gacggcgtca tcctgactcc gccccattcg gacaacccgc tgattataga
     2341 cctgctggtg caactggggt tgcctttcgc ccgcatcggc tctgtcgcgg agggaccggg
     2401 gttcgccatc accatggatg accgtgcggc gggcagggcg gctaccgatc acctgctcga
     2461 tctcggccac acgcgggtcg gcttcatcac cggcagtgac gaatgggcca tcagcggcgc
     2521 ccgtctggac gggtacaaac aggcgatcct tggccggggg ctcgagctcg acgaggcgct
     2581 gatcgggcgt ggcgacttct cgttcgagtc cggaactgcg gctatggaag ctttctacga
     2641 attggctgca cctccaaccg ccctcgtcgc cagcaacgat cagatggcgc tggccgcctt
     2701 gcgcgtcgcc aatgggcggg ggatgaagtc tccggatcac ctggccctca tcagcttcga
     2761 cgacacgccc atcgtcaaat tcagcaaccc gccgctgacg gccatcgtcc agcccatcgc
     2821 agtcatggcg gccgaggccg cgcggctcct gattcaggcc aaggccggtg aggcggattt
     2881 gcccaccagc gtcgtggtgc cgttcagcct ggcgatacgg gactccactc ggcgcgcctg
     2941 aggccctaaa ccgcctgaaa agagacttcc ggaggcgacc ccaatggcga atggcttgag
     3001 tacgtccgca cgacactatc tgccgggctt cggcaaccac ggcgtcagcg aggccgtgcc
     3061 gggcgcattg ccgcagggcc agaactcgcc gcagcgcgcc ccgttcggtc tctacgccga
     3121 gcagctgtcg ggcacggcct tcaccgcccc ccgccatgag aaccgccgca gttggctgta
     3181 ccgactgcgc cccaccgcca accatccgcc gttcacgccg tggacctcgg ctaccttgct
     3241 taagtcggga ccgttcgagc ggctgccaac gccgaaccgg ctgcggtggg atcctctggc
     3301 gatgccgagc gcgccgacgg atttcgtcga ggggctggtg acctatggcg gcaacggcga
     3361 cgtgggccag accgccggca tcggcatcca tctgtacgcc tgcaaccggt cgatggagaa
     3421 ccgggtcttc ttcaacgccg acggcgagct gctgatcgtg ccgcaggagg gcgggctggt
     3481 catcacgacc gagttcggga tcatcgacct cgaaccgcag cagatcgcgg tcatcccgcg
     3541 tggcgtccgc ttccgtgtcg ccctcaccag ccagcaggcg aggggctacg tctgcgagaa
     3601 ctacggcgcc ctgttccgcc tgccggatct ggggcctatc ggctccaacg ggctggccaa
     3661 tccgcgcgac ttcgaaaccc ccgtggcggc ctatgaagat ctcgacgggc cggtcgagct
     3721 gatccagaaa ttccagggcg agctgtggtc aacgacgctg gatcattcgc cgttcgacgt
     3781 ggtggcctgg catggcaacc tggcgccgta ccgctatgat ctgcggcgct tcaacacgat
     3841 caacacggtc agctacgacc accctgaccc gtcgatcttc accgtcctga ccagcccgtc
     3901 ggacacgccg gggaccgcca actgcgactt cgtcatcttc ccgccgcgct ggatggtggc
     3961 cgaggacacg ttccggccgc cgtggttcca tcgcaacgtc atgagcgagt tcatgggcct
     4021 gatcctcggc gcctatgacg ccaaggaagg cgggttcgcg ccgggcggcg cctcgctgca
     4081 caactgcatg agcggccacg gtcccgatca ggccagctac gccaaggccg tcgaggccga
     4141 gctgaagccg cacaagatcg agaacaccat ggccttcatg ttcgagagcc gctgggtcat
     4201 ccggcccacg gatcacgcca tggacagtcc gacgatgcag ctcgactacg acgacgtctg
     4261 gtccgacttc gacaagggcc agccggttta agaggggccg accggctgaa cccgctactc
     4321 ggcggcttca gcctcggcgc gcaccgggag ggtccagttc gggcggatga agtggcaggt
     4381 gtagccgtcg ggcaggcgct ccagatagtc ctggtgctcg ggctcggctt cccagaacgg
     4441 accggccgcc gtcacctcgg tcaccacctt gccgggccat atgccggagg cgtcgacgtc
     4501 ggcgatggtg tccagcgcga cctgcttctg ctcgtcggag gtatagaaga tcgccgaacg
     4561 atagctgagg ccgagatcgt tgccctggcg gttcagggtc gagggatcgt ggatctggaa
     4621 gaagaactcc agcaggcggc gataggtgat gagctcggga tcatagatga tctcgatggc
     4681 ctcggcgtgg gtgccgtggt tgcgataggt ggcgttcggc acgtcgccgc cggaatagcc
     4741 gacgcgggtc tcgatcacgc ccggatagcg acgcagcagg tcctgcacgc cccagaagca
     4801 gcctccggcg aggatcgcgg tttcggtctt ggccatggtg gtcggtcctt cgttttcggg
     4861 tttccagata gataggcgat gcggcggcgc tgaaaagggc caagctgcca agcgaacctg
     4921 catcgccccc aactaaatcg cgctttggga ccccgtgagg gcgatccggc gcttgccgac
     4981 gggcacgggc tgttctatca gcctcgcctc ccgccggaga gaccatccgg cgctgcgaag
     5041 gctctctggg cgatcgtgga aacggacagg tggcggagtg gtcgatcgcg cacgcttgga
     5101 aagcgtgtgt aggtgaaagc ctaccgaggg ttcgaatccc tctctgtccg ccacgatgcc
     5161 tgctttcccg tcatcaggtt cgccgcttcg acccgtccgc gagggtcgag agtccactgt
     5221 gtcccgaaaa atcgaaaatg gagacactgg accctccggg ggccatgtgc cgtttccgcc
     5281 gggatcggcg ggcgggcgca aggatgggct atcgcgcctg gaatcggctc ggtcgcgcag
     5341 tgaaatagcg atgtgaaaga ccctgagggc ggctgcagag cggctcgcgt acctcatctt
     5401 ctacaaggta gtgaagcgct gcggttcaag gcctctgcgg cgaataaaag cggcgccgta
     5461 agggggcgaa tgtctccaca agggtcgcga aggggcgaga atatcagggt cgagagtctc
     5521 gcaattttgg ggggtgttta ctcggtcgag gggcggacag ttccgagtcg cgagcagccc
     5581 catctaacgc ttccgtggac gcctcttcat cgcgccgaca ctactggcgt cagaccaggc
     5641 acaatgatcc gacgccatcc gggggtatta tgtagaaacg tggcgctatt gccgggacgc
     5701 caccaggcga gtgatgtgcg ccggtgcgct aactttaaga tcaagccagt gtataacgtt
     5761 gtagtataga tatgattgat ccatatactg tctcagtatg actgagaccc tatcgcgaga
     5821 taggcactcg catactttta cggcggacca tttcctcatt tcgtcgttga cttcatttag
     5881 aagagatatg atggcttcat tcgacagaga cctaaaggcg ttctgcgggt agtttttgat
     5941 aatcgcgctc ttaatgccaa agtcaatgtc aagctggatg cattctaaga gcctttgacc
     6001 ggcaatcttt cggatcgtca ctgccaactc gtcattgctg acggtgctgt cagagaacag
     6061 ccccatgcca gtcgatcttg tcgcgctggc gatgagcatt atgtcggacc actcgccatg
     6121 cttaccaaag aatgcgagcg cgctcggttg gattatataa tatttgctag atacggcaga
     6181 tctgaaaaat cgaatgtccg atgcgtcgcc cttttttgca aagacttcca aggtcgattt
     6241 gatcatgttc gaagcaacga attccatgag tccttcattg ggaatcatag actctgcccg
     6301 cgtctcacca aatctcgcta agtagctctt tttgatagtg tcgaaatagc ttacaaagct
     6361 atccgttaga tttgcacggg cagttttgct tctctgtttc cagtgccgta aaagtagcgt
     6421 tgaatatgcc acgttcgcgg gcgtgaagaa gctgtctagc tcactctcca agtcatcatc
     6481 aggcattgcc ttgaaaacga tttctcggag tttcgaatat tcctcttcgc ctacttttct
     6541 ctttggaaga aaggcgtttg tctccgtctg aaccacaatc ttcgcgatgt cgccgagaga
     6601 caaggattcc ccagcttgaa gggcgacttc gactgcagcc atccgaacct tagcggacgg
     6661 gtctgttcgc aggtctgagc taagttcggg cgcaagaggc ttgcgggttg ccaagactcg
     6721 cacggccatc gctcgcacgt cagcgtctcg atgcgacgcg cccctttcga cgacgttgtc
     6781 cgacaactcg gtcattgact tgacgacggt gtcccggctt cgccattcgc cactaggaaa
     6841 cctgttttcg tcgatgaggt agacaagcgc atca
//
LOCUS       DASVTM010000366         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_990898,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000366 DASVTM010000000
VERSION     DASVTM010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990898"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF633_09965"
     CDS             <1..247
                     /locus_tag="VF633_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX8471427.1"
                     /translation="AFPGGRHRLEAASEAGRSAGVVVGGVVVMLFVAGLLEGFGRQLI
                     TDTGARYAIGIVTGVLWGTYFYASQRGPSAERPLNVR"
     gene            306..1157
                     /locus_tag="VF633_09970"
     CDS             306..1157
                     /locus_tag="VF633_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX8471428.1"
                     /translation="MREFVTPEGVDLRLNIGDAGQRAGAFLLDAAIILAILVGLTIAA
                     IFMGIGAAAAGGGEAGMEMVGVVWLLGFFLLRNFYFTAFELSARAATPGKRMMGLRVA
                     SRDGGRLRAESVFARNAMRELEVFLPLTLLLGQAKEGGVESWMYILGFIWAAIFVFFP
                     LFNRDRLRCGDLVGGTWVVRNPRHKLTRDMADDGSARLNRFDFAPAQIDAYGAKELHI
                     LEDVLRLRNRRVMRSVAERIAAKIAFPHTPFDDDYDFLSAYYAALRGRLESRLLMGVR
                     RHDKHDV"
     gene            complement(1166..1933)
                     /locus_tag="VF633_09975"
     CDS             complement(1166..1933)
                     /locus_tag="VF633_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471429.1"
                     /translation="MTTTDYQGGGFDLGRVVQRTFASISQNWLVFLVAAIALVGIPSL
                     LTGFGQSMSTTVQPLAGLPFISIGWILMIIGGYVLQGTVVFTTVNGFNGKQVDIGSAL
                     TVGVRFFFPLLAVAILMWLGMVIGLILLIVPGLILLVMWAVTAPVLVAEKRGILESFQ
                     RSRDLTRGNRWMIFALFLVYGIISSIIGWTVIGLGAAMGGSLATASTVWLVALVFTPM
                     VNTVTAVLSAAGVASIYYELRTAKEGVSPDQLASAFD"
     gene            complement(2031..2774)
                     /locus_tag="VF633_09980"
     CDS             complement(2031..2774)
                     /locus_tag="VF633_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8471430.1"
                     /translation="MSQTSISDDTPQISVVVPVHDEAGAVGQLARDIAAAFAGRSYEM
                     IFVDDASRDTTLAELRALMVELPALRVLAHASNAGQSRAVRTGVLAARGAVIVTLDGD
                     GQNPPHDAPRLADALIAAPANVGLVGGVRARRQDSGAKRQASLWANRIRRKLLNDDAD
                     DTGCGLKAFRRDLFLRLPYFDHIHRYLPALMIREGFENRYLEVDHRHRETGYSKYTNW
                     GRLMASFSDLLGVIWLKTRSRQPGPVSEF"
     gene            2899..>3831
                     /locus_tag="VF633_09985"
     CDS             2899..>3831
                     /locus_tag="VF633_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX8471431.1"
                     /translation="MTRPNLDRYIAGWRGPLLAALLTLVAGLPGLLLLPPLDRDESRY
                     AQATSQMLESRDYVDIRFQNEPRWKKPVGIYWMQAAAVALTSKVENRDIAPYRLPSLF
                     GAMVAALAVAWAGAALFGQRAGFLAGAILGTTFLLSAEGGIAKTDAVLCGCVTLAMAA
                     LARIYMAVRAGEVPRRPHKFLFWLGLGLSILIKGPIGLIVVVPAMIALSIWDRDIKWM
                     KRLGWGWGLPLVALLVGPWAIAITIATDGGFWREAVGNDLGGKITGAAESHGGFIGMY
                     AVLAPLLLFPATLLLPAALSTGWHRRAEPAVRFMV"
BASE COUNT          653 a         1216 c         1286 g          676 t
ORIGIN      
        1 cgcgttcccc ggcggccgtc accggctgga agcagcatcc gaagcaggtc gaagcgctgg
       61 cgtagtcgtc ggcggggtgg tggtcatgct gttcgtcgcc gggttgctgg agggcttcgg
      121 tcgccaattg atcaccgaca ccggggcacg ctacgcgata ggcatcgtca cgggagtttt
      181 gtgggggaca tatttctacg cttcacagcg tggtccttcc gccgaaaggc cgctgaatgt
      241 ccgctgaaac cgctatcacg aaccggcctg tccggccggc tagcaaggcg ccggccgacc
      301 ggaccttgcg cgagttcgtc acgcccgagg gcgtcgatct tcgcctgaat atcggcgacg
      361 ccggccagcg ggccggggcc ttcctgctcg acgccgccat cattctcgcg atcctggtcg
      421 gattgaccat cgcagcgatt ttcatgggta tcggcgccgc agcagcgggg ggcggcgagg
      481 caggcatgga gatggtgggg gtcgtctggc tgctcggctt ttttctgttg aggaatttct
      541 acttcacggc gtttgagctt tcagcgcggg ccgccacgcc cggcaagcgg atgatggggc
      601 tgcgggtcgc atcgcgcgat ggagggcggc tacgggccga gtctgtcttc gcgcgtaacg
      661 ccatgcgcga gctcgaggtc ttcctgcccc tgaccctgct tttgggtcag gccaaggagg
      721 ggggcgtcga gagctggatg tacatcctcg gcttcatctg ggcggcgatc ttcgtatttt
      781 tcccgctttt caaccgcgac cgcttgcgtt gtggcgatct ggtcggcggc acctgggtgg
      841 tgcgcaatcc gcgccacaag ttgacccgag acatggccga cgacggcagc gccaggttga
      901 accgtttcga ttttgccccg gcccagatcg acgcctatgg ggccaaggag ctacatattc
      961 ttgaggatgt actgcgcctg cgaaaccgcc gcgtgatgcg ctctgtcgcc gagcgtatag
     1021 cggccaagat cgcctttccg catacgccgt tcgacgacga ctacgacttc ctcagcgcct
     1081 attatgccgc gctgcgggga cggctggagt cccgcctgct gatgggcgtc agacgtcacg
     1141 acaagcacga cgtctgacgc gaacctcagt cgaacgccga ggccagttgg tccggcgata
     1201 cgccttcctt ggcggtgcga agctcgtaat agatcgacgc gacgcctgcc gccgagagca
     1261 cggcggtgac cgtattgacc atgggcgtaa acaccaacgc caccagccag acggtactgg
     1321 cggtcgccag cgagccgccc atcgcagcgc cgaggcctat gacggtccag ccgatgatcg
     1381 acgagatgat gccgtagacg aggaaaagcg cgaaaatcat ccagcgattg ccgcgagtta
     1441 ggtcacggct gcgctggaaa ctttcgagaa tgccacgttt ctcggcgacg agtacggggg
     1501 cggtaaccgc ccacatcacg agcaggatca ggccgggaac gatcaacagg atcaatccga
     1561 taaccatgcc caaccacatc agtatcgcca cggcgagcag cgggaagaag aacctaacgc
     1621 ctaccgtcaa ggcgctgccg atatcgactt gcttgccatt gaagccgttc acggtcgtga
     1681 agacgaccgt cccttgcagg acgtagcccc cgatgatcat gagtatccaa cctatcgaga
     1741 tgaacggcag acccgccagg ggctggaccg tagtgctcat gctttgacca aaaccggtga
     1801 gcagactcgg aataccgacc agcgcaatgg cggcgaccag aaagaccagc cagttctggc
     1861 taatggacgc gaacgtccgc tgcacgacgc gaccgagatc aaacccgccg ccctgatagt
     1921 ctgttgtcgt catggaaccc ctcctgatgt ggagggcaga agagccggtc gcgtgcgacc
     1981 tgacaagggc gcactgagag ttgacggtta gacctgttgg gcccgacgcc ttagaactcg
     2041 ctcaccggcc cgggctggcg gctgcgggtc ttgagccaga tcacgcccag caggtccgag
     2101 aacgaggcca tcagccgccc ccagttggtg tatttcgaat agccggtctc gcggtggcgg
     2161 tggtcgacct caagatagcg gttttcgaat ccctcgcgga tcatcagggc cggcagatag
     2221 cggtggatgt gatcgaaata gggaaggcgc agaaacagat cgcggcggaa ggccttgagc
     2281 ccgcatcccg tgtcgtcggc gtcgtcgtta agcagcttcc tgcgaatgcg gttggcccac
     2341 agagaggcct ggcgtttggc acccgaatcc tgacgccgag cacgcacgcc gcccaccaga
     2401 ccgacgttgg cgggcgctgc gatcagggcg tcggcgaggc gcggggcgtc gtgcggcgga
     2461 ttctggccgt cgccgtccag cgtcacgatg acggctccgc gcgccgcaag gacgcccgtg
     2521 cgcacggccc ggctctgacc cgcgttggag gcatgggcca ggacgcgcag cgcgggcagt
     2581 tcgaccatca gggcgcgcag ctcggccagc gtcgtgtcgc ggctggcgtc gtcgacgaag
     2641 atcatctcgt aggaccggcc cgcaaaggcg gcggcgatgt cgcgcgccag ttgcccgacg
     2701 gcgcccgcct cgtcatgcac cggaacgacg acagagatct ggggggtgtc atcggagatc
     2761 gaggtctggc tcatcgccgc tgtttagccg agcccgtcgt ctcaagcgag ggtgtgtggc
     2821 acgcgccgtc agcccgttgc tcagcaaggt taaagagggg cctgacacga gcgcgcgaac
     2881 acgtgctacg acagtcacat gacgcgcccg aacctggatc gctatatcgc cggctggcgc
     2941 ggccccctgc tggcggcgct actgaccctt gtcgccggct tgcccggact gttgctgctg
     3001 ccgccgctgg atcgcgacga gagccgctac gcccaggcca cctcgcagat gctggaaagc
     3061 cgcgactacg tcgacattcg cttccagaac gagccgcgct ggaagaagcc cgtcggcatc
     3121 tactggatgc aggccgccgc agtcgccctg acgtccaagg tcgagaaccg cgacatcgcc
     3181 ccctatcgcc tgccgtccct gttcggcgcg atggtggcgg cgcttgcggt cgcgtgggcc
     3241 ggtgcggcgc tgttcggcca gagagccggc tttctggccg gcgccatcct cgggaccacc
     3301 ttcctgcttt cggccgaggg agggatcgcc aagaccgacg ctgttctctg cggctgtgtc
     3361 accctggcca tggcggcgct ggcgcggatc tatatggcgg tgcgggccgg cgaggtccca
     3421 agacgaccgc acaaattcct gttctggctc gggctcggcc tgtcgatcct gatcaagggg
     3481 ccgatcgggc tgatcgtcgt ggtcccggcc atgatcgccc tgtcgatatg ggatagagac
     3541 atcaagtgga tgaagcggct gggctggggc tggggcctgc cgctggtcgc cctgctggtc
     3601 gggccgtggg ccatcgccat caccatcgcc accgatggcg gcttctggcg cgaggccgtg
     3661 ggcaacgatc tcggcggcaa gatcaccggc gcggccgaga gccatggcgg cttcatcggc
     3721 atgtacgcgg tcctcgcgcc gctgctgctg ttccccgcga ccctgctgct gcccgccgcg
     3781 ttgtcgaccg gctggcatcg tcgggcggag cctgcggtgc ggttcatggt c
//
LOCUS       DASVTM010000367         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1045275,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000367 DASVTM010000000
VERSION     DASVTM010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045275"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF633_09990"
     CDS             complement(<1..559)
                     /locus_tag="VF633_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471432.1"
                     /translation="MKRNVVSLCFGVAAQALIGAMAVGPVLAQTGPTAPAGSDWPTYG
                     HDKGGMRFSPLTQITPANVASLQPAWVFHMRPAGAAPGATAQGAAAAAQAVSEGIAPP
                     PGAPATAPAVAAGAPGPQGRPGGGGGMFGPNANGFAASQATPLMVGGLMYLTTPYKRV
                     VALEPETGKEVWSFEVDGTGQPSQRG"
     gene            831..2147
                     /locus_tag="VF633_09995"
     CDS             831..2147
                     /locus_tag="VF633_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471433.1"
                     /translation="MAHIGATTGKRSNARFLILALVTVGTMINYLDRSILGVAAPVMT
                     KELGIDAAVMGIVFSAFSWTYAASQIPGGVFLDRFGTRLTYSLSVSFWSLFTLLQAFV
                     GGVGTLLLFRLGLGVAEAPCYPANSRILSTWFPQHERARATGVYSVGQYFGLAFLSPL
                     LFWITAAFGWRMLFFIIGAAGIAFGVLWWFVYREPHEAKQANQAELDYIEAGGGLAHK
                     GALTKFDFRDIGRLLSKRQILGASIGQFAGNSTLVFFLTWFPTYLATERGMSWLKVGV
                     YAVMPFICASIGVFLGGIVSDYLLKKTGNATLARKLPIVAGLLLASCIVGAAFVDDNR
                     MVITIMSIAFFGQGMTNLGWTLISDVAPKRLIGLTGGVFNLCANMAGILTPLVIGFVV
                     AATGSFFGGLAFISVVALIGVLSYVFILGPVVRVEIDLPSEAEGAQ"
     gene            2150..>2465
                     /locus_tag="VF633_10000"
     CDS             2150..>2465
                     /locus_tag="VF633_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471434.1"
                     /translation="MQPPSPVSSGAAESRPGDQLRVGLIGSGIGTSRTPAMHMREAAE
                     AGLDYDYVLMDLDGEPDGADALPRLLDQAQKAGFRGLNITHPCKQSVIPLLDEMDDDV
                     RVI"
BASE COUNT          397 a          823 c          772 g          473 t
ORIGIN      
        1 cgccgcgctg tgaaggttga cccgtgccgt cgacctcgaa cgaccagact tccttgcccg
       61 tctcgggttc gagggccacg acgcgcttgt agggcgtggt cagatacatc agcccaccga
      121 ccatcagcgg cgtggcctgc gaggcggcga acccgttggc gttggggccg aacataccgc
      181 ctcccccgcc cggacgacct tgcgggcccg gagcgcctgc ggccacagct ggagcagtcg
      241 ccggagcgcc cggaggcggt gcgatccctt cagacacggc ctgcgccgct gcggctgcgc
      301 cttgagccgt cgcgccgggc gcagcgcctg cggggcgcat atggaatacc caggcgggtt
      361 gcagagatgc gacgttcgcc ggcgtgatct gggtcagggg cgagaaccgc atgccgccct
      421 tgtcgtgacc ataggtcggc cagtcggaac ccgcaggcgc cgtcggcccg gtctgggcca
      481 gcacagggcc gaccgccatc gcaccgatga gcgcttgagc cgcgacacca aagcaaaggg
      541 atacaacgtt ccgcttcata accgtctccc ggacaacagc ctgccgacgc agcgcgtggc
      601 gaaagccgtc atcgaatgtg catttccaat gggcgatctg ttgccgatcg cttccttgcg
      661 gcgattaaca ccggacgatc ggtcttgatc aagcgcattt tgtacgaaag agtgagttca
      721 gcgccgaaca acggcgacga gcgatcgccg aagcccttct acaggcgcgg tgatcgacct
      781 caaacataag ccggcgggtc aatcccggcg ggcattagga gagagacggc atggcgcaca
      841 tcggcgcgac gacaggcaag cgcagcaatg cccggtttct gattctggct ctcgtcacgg
      901 tcgggacgat gatcaactac ctcgaccgat ccattctcgg ggtggctgcg cccgtgatga
      961 ccaaggagct gggaatcgac gccgccgtca tgggcatcgt cttctcggcc ttctcctgga
     1021 cctatgcggc gtcgcagatc ccgggcggcg tgttcctcga ccgcttcggc acgcgactga
     1081 cctattccct ttccgtcagc ttctggtccc tgttcaccct gctgcaagct ttcgttggcg
     1141 gtgtgggaac gctgctgctg tttcgccttg gtctgggtgt cgccgaggcg ccctgctatc
     1201 cggccaacag tcgcatcctt tcgacctggt ttcctcagca cgaacgggcc cgggcgaccg
     1261 gcgtttattc cgtcggccag tatttcggcc tcgccttcct gagccccctg ctgttctgga
     1321 tcacggcagc tttcggctgg cgcatgttgt tcttcatcat cggcgcggcc ggcatcgcct
     1381 tcggcgttct ctggtggttc gtctatcgcg agccgcatga ggccaagcag gcgaaccagg
     1441 ccgagctcga ctacatcgaa gcgggcgggg gtctggctca caagggcgcc ctgaccaagt
     1501 tcgacttccg cgacatcggt cgtctgctgt ccaagcgcca gatcctcggc gcctcgatcg
     1561 gccagttcgc cggcaactcg acgctggtgt tcttcctgac ctggttcccg acctatctgg
     1621 cgaccgagcg gggcatgtcc tggctcaagg tcggggtcta tgccgtcatg cccttcatct
     1681 gcgcctcgat cggcgtcttc ctcggcggca tcgtttccga ctacctgctg aagaagaccg
     1741 gcaacgccac attggcacgc aaactgccga tcgtcgccgg actgctgctg gcgtcctgca
     1801 tcgtcggtgc ggcattcgtc gatgacaacc ggatggtgat caccatcatg tcgatcgcct
     1861 tcttcggtca gggcatgacc aacctcggct ggacactgat ttccgacgtc gcgcccaagc
     1921 ggctgatcgg tctgacgggc ggcgtgttca acctgtgcgc caatatggct ggcatactga
     1981 cgccgctggt catcggcttc gtcgtcgccg cgaccggctc cttcttcggc gggctggcct
     2041 tcatcagcgt ggtcgccctg atcggtgtcc tctcctacgt ctttatcctg ggaccggtcg
     2101 tacgcgtcga gatcgacctg ccgtccgaag ccgagggcgc gcagtagaga tgcagccccc
     2161 ctccccagtc tcgtcgggcg cagccgaaag ccgacccgga gatcaactcc gggtcgggct
     2221 gatcggatca ggcatcggaa cgtcgcgcac gcccgcgatg cacatgcgcg aggctgcgga
     2281 ggcggggctg gactacgact atgtgctgat ggatctggat ggtgagcctg acggcgcaga
     2341 cgccctcccc cgcctcctcg accaagccca gaaggcagga ttccgcggcc tgaatatcac
     2401 ccacccctgc aagcaatcgg tgatcccgct gctggacgag atggacgacg acgttcgcgt
     2461 catcg
//
LOCUS       DASVTM010000368         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1045294,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000368 DASVTM010000000
VERSION     DASVTM010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045294"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VF633_10005"
     CDS             complement(<1..493)
                     /locus_tag="VF633_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX8471435.1"
                     /translation="MSNDEKPPKAPIVAEPEPARRKMVRPPTGGTAQGRGMGTQIKTA
                     DKKSMSSIQWIKRQLADPWSERARAEGWRSRAAFKFSEIDDRFHLLKKGSRVIDLGAA
                     PGGWIQVCVNRGAAAVVGVDLLPIEPIPGSILIQADFTDAGVDQQLIDALGGPPDIVL
                     SDMA"
     gene            577..1026
                     /locus_tag="VF633_10010"
     CDS             577..1026
                     /locus_tag="VF633_10010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8471436.1"
                     /translation="MADETLTLSLDTGDVVIKLRPDLAPGHVARITELAKEGFYDGVV
                     FHRVIPGFMAQGGDPTGTGTSGSKKPNLKAEFSKEPHVRGVCSMARTSQPDTANSQFF
                     ICLDDAGFLDGQYTVWGEVIEGMDHVDALPKGEPPRAPGKIVKATVA"
     gene            complement(1078..1626)
                     /locus_tag="VF633_10015"
     CDS             complement(1078..1626)
                     /locus_tag="VF633_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471437.1"
                     /translation="MIDRHAMKCAPVLAAMTLFVALAACDDVKTPASTAEAPAKSVTA
                     AFTHQISSDLSGYYIPATPISVDGYRVKALFLGQGADFAAWEGGAKSGGFAPVMIEFE
                     KNGATPIRVLPQTYSVSDGRVRFEGVDPRLGNVAFTGTLDQNALATARRNLGDEAPVL
                     TGTLRAGGTAFGHQKFRWYGGD"
     gene            complement(1623..2246)
                     /locus_tag="VF633_10020"
     CDS             complement(1623..2246)
                     /locus_tag="VF633_10020"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8471438.1"
                     /translation="MTATTGFDLQHIQRVRKWAIGLSLLAAVGLSMFTGTQWGDGMFH
                     EGVEQFGVAAIVISIVGRAWCSLYIGGRKKAEIVATGPYSISRNPLYLFSHIGAVGIG
                     AQTGSVTLALLFMAVSVVVFHFTVRREELWLLQAFGAPYADYMRRVPRNGPDFSRWHD
                     EASLEIRPQFFLTTIRDGLVFLLAVPIFEGIEHMQAIGWLATLLRLP"
     gene            complement(2324..3289)
                     /locus_tag="VF633_10025"
     CDS             complement(2324..3289)
                     /locus_tag="VF633_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8471439.1"
                     /translation="MMRRAVPLAFTISIVALLAGCQTEAPTASAPTQAPAIPAASQQL
                     LDDVRILSADDMEGRDTASPGGERARTYIVSRLEAMGVEAPPVGRLQPFEAEVPSRSG
                     LMRYKGTNIIGLIPGTRVADRYIVVTAHYDHVGISGGQIYNGADDNASGVATMLEIAR
                     RLKVQPPEHSVVIVAFDGEERGLIGAKHFVQAPPVPLTSIALNLNFDMTSRAETDGHL
                     WVTGTYQNPTLRPILQGIPANGAISLSFGHDTPQDTGTDNWVEASDHGPFFKANVPFL
                     YMGVVDHPDYHRPSDDFERITPSAFISSTELAAAAFRALDQSLDR"
     gene            3422..>4283
                     /locus_tag="VF633_10030"
     CDS             3422..>4283
                     /locus_tag="VF633_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX8471440.1"
                     /translation="MAQTNPQASLPVEDLTEAQAAEELARLAAEMRRADFLYNEAAPE
                     LTDADYDALKARNLAIETRFPTLIRADSPSLKVGAPTSSQFSEVRHGVPMLSLDNAFD
                     DGDVTDFVARIQRFLKLPHADPVAFVAEPKIDGLSANIRYEHGILVQGATRGDGRTGE
                     DITANLRTIRDIPPKLAGSGWPDLIEVRGEVYAPNEGFAAFNAAAEAEGRRTYANPRN
                     FAAGTLRQLDASIVASRRLDMFPYDLLAGNQKAFPTHWAIFEWLAAKGFNVNPNRALC
                     ADFDELTEFIG"
BASE COUNT          801 a         1394 c         1343 g          745 t
ORIGIN      
        1 gggccatgtc cgacagcacg atgtccggcg gaccgccaag cgcgtcgatc agttgctggt
       61 ccacgcccgc gtcggtgaag tcggcctgaa tcaggatcga tccgggaatc ggctcgatcg
      121 gaagcaggtc cacaccgacg accgcggcgg cgccgcgatt gacgcacacc tgaatccagc
      181 cgccgggagc cgcgccgagg tcgatgaccc gcgagccctt cttcaacagg tggaaccggt
      241 cgtctatctc gctgaatttg aaggccgcac ggctacgcca gccttcggcg cgggcgcgtt
      301 ccgaccaggg atcggccagc tggcgcttga tccactggat ggacgacatc gacttcttgt
      361 cggccgtctt gatctgggtt cccatgcccc ggccttgggc cgtgccgccg gtcggtggac
      421 gcaccatctt gcgtcgcgcg ggctcgggtt cggctacaat aggcgccttg gggggctttt
      481 cgtcattact catgcttccc acatagcccc cacggccgtc acgggctatg acccccgacc
      541 ggaatagaag aaccgaactt gaaacggagc gcgcctatgg ccgacgaaac actgacccta
      601 tccctcgaca ctggtgacgt ggtcatcaag ctgcggcccg atctggcccc cggccacgtg
      661 gcccgcatca ccgaactggc caaggaaggc ttctacgacg gagtggtctt ccaccgcgtc
      721 atccccggct tcatggccca gggcggcgat ccgaccggca ccggcacctc gggctccaag
      781 aagccgaacc tgaaggcgga gttctccaag gaacctcacg tccgcggcgt ctgctcgatg
      841 gcccgcacct cacagcccga caccgccaac tcgcagttct tcatctgcct cgacgacgcc
      901 ggcttcctcg acggccagta caccgtctgg ggcgaggtca tcgaaggcat ggaccatgtc
      961 gacgccctgc cgaagggtga accaccccgc gcgccgggca agatcgtcaa ggcgaccgta
     1021 gcctaaacga ctaccaatgt ttctggaggg cgggtcgcga gacccgccct ttctgattca
     1081 atcaccgccg taccagcgga acttctggtg gccaaaggct gtcccgcctg cgcgtagggt
     1141 cccggtcagc accggggcct cgtcgccgag attgcgccgt gccgtagcca gcgcgttctg
     1201 atcgagcgtt ccggtgaagg cgacattgcc caaccgggga tcgacgccct cgaatcggac
     1261 ccgaccgtcg ctgacggaat aggtttgggg caacacgcgg atcggcgtgg ctccgttctt
     1321 ctcgaactcg atcatcaccg gtgcgaatcc gccggatttc gctccaccct cccacgccgc
     1381 aaagtccgca ccctgaccga ggaagagcgc ctttaccctg tagccatcaa ctgagatggg
     1441 ggtggcggga atgtaatagc cggacaggtc gctgctgatc tggtgtgtga aggcggccgt
     1501 aacggacttt gccggagcct cggccgtaga tgcgggcgtc ttgacgtcat cgcaggcggc
     1561 aagcgcgaca aacagcgtca ttgcggccag tacgggtgcg catttcatcg cgtgtctgtc
     1621 gatcatggca accggaggag ggtcgcgagc caaccgatcg cctgcatatg ctcgatacct
     1681 tcgaaaatgg gcacggcgag caagaagacg agaccgtcgc ggatcgtggt cagaaagaac
     1741 tgcggtctga tctccagtga agcttcgtca tgccatcggg agaaatccgg cccgttgcga
     1801 ggaacgcggc gcatgtagtc tgcatagggt gcgccgaatg cctgaagcag ccagagctcc
     1861 tcacgacgga cggtgaagtg aaacaccacc accgacacgg ccatgaacag cagagccagg
     1921 gtcacgctgc ccgtctgggc tccgatgccc accgccccga tgtgactgaa cagatagagg
     1981 ggattgcggc tgatcgaata aggacccgtg gcgacgatct ccgccttctt gcgtccaccg
     2041 atgtacaacg aacaccaggc ccgcccgacg atggagatca cgatggcggc gacgccgaac
     2101 tgctcgacac cctcgtggaa catgccgtca ccccactggg tgcccgtgaa catcgacagt
     2161 cccacggccg ccaacagact gagcccgatg gcccatttcc gcacgcgctg gatgtgttga
     2221 aggtcgaatc cggtcgttgc cgtcatgttt ttgctctccc gcgccgcgag atgagcgcct
     2281 gacggacgct ccgtgcaagc cctatgtttg caaccgaaat gcgttaccga tccagcgact
     2341 ggtcgagcgc ccgaaacgcg gcggcggcga gttcggtcga actgatgaag gccgagggtg
     2401 tgatgcgctc gaagtcgtcg gacggccggt gatagtcggg gtgatcgacg acacccatat
     2461 agaggaacgg gacattggcc ttgaagaacg ggccgtggtc cgaggcctca acccagttgt
     2521 ccgtgccggt gtcctgtggc gtgtcatggc cgaacgacag tgagatcgcg ccgttggcag
     2581 ggatgccctg caggatcgga cgcagcgtcg ggttctggta ggtcccggtc acccatagat
     2641 gaccgtcggt ctctgcgcgc gaggtcatgt cgaagttcag gttgagggcg atggaggtca
     2701 ggggcaccgg cggcgcctga acgaaatgct tggccccgat cagccccctc tcctccccgt
     2761 cgaaggcgac gatcacgacg ctgtgttcag gtggttgaac cttcaggcgg cgcgcgatct
     2821 caagcatggt ggcgacgccc gaggcgttgt cgtcggcgcc gttatagatc tggccaccgc
     2881 tgatcccgac gtggtcatag tgggcggtga cgacaatgta gcgatccgcg acccgcgtcc
     2941 ccgggatcag gccgatgatg ttggtcccct tatagcgcat caggcccgac cggctgggaa
     3001 cctcggcctc gaacggttga agacgaccga cgggcggggc ctcgaccccc atcgcttcca
     3061 gccgcgagac gatataggtc cgcgcccgct caccaccggg cgatgcggtg tcgcgcccct
     3121 ccatgtcgtc ggccgacagg atacgaacgt cgtcgagcag ttgctgcgag gccgccggga
     3181 tcgccggcgc ttgtgtcggg gcggaagcgg taggagcttc ggtctggcag cccgcaagca
     3241 gggcaactat cgaaatggtg aaagcaagcg ggacggcgcg acgcatcatg aggtcttcct
     3301 attacggatg ccgaccctaa cgttcgccgc tgggccggtc agcatacggt tctgtcatca
     3361 gcagcaaaaa tcccatcatc gggagtccat tcctcccccg cgagggtggt agaaaccctc
     3421 gatggcccag acgaatccgc aagcatccct cccggtcgag gacctgactg aagcgcaggc
     3481 ggccgaggag ttggcccggc tggcggcgga gatgcgacgc gccgacttcc tctataacga
     3541 ggcagcgccc gaactcaccg acgccgacta cgatgccctg aaggcccgaa acctggccat
     3601 cgagacgcgt ttcccgacgc tgatccgcgc ggattccccg tcgcttaagg tgggcgcacc
     3661 gacgtcgagc cagttctccg aggtgcgtca cggcgtgccg atgctgtcgc tcgacaacgc
     3721 cttcgacgac ggcgacgtca ccgacttcgt cgcccgtatc cagcgcttcc tgaagctgcc
     3781 gcatgccgat cctgtagcct ttgtcgccga acccaagatc gacggtcttt ccgccaacat
     3841 ccgctatgaa cacggcattt tggtccaggg cgcgacgcgc ggcgacggcc ggaccggcga
     3901 ggacatcacg gctaacctgc gcacgattag agacatcccg cccaagctcg cgggctccgg
     3961 ctggcccgac ctgatcgaag tgcggggcga ggtctacgcc cccaacgaag gttttgcggc
     4021 cttcaatgcg gcggcggaag ccgagggtcg ccgcacctac gccaacccgc gcaatttcgc
     4081 cgccggaact ttgcgccagc tcgacgcgtc catcgtcgcc tcgcgccgtc tcgatatgtt
     4141 tccgtatgat ttgctcgccg gaaatcaaaa agcgtttccg acgcattggg cgattttcga
     4201 gtggctcgcg gcgaaaggtt tcaacgtcaa tccgaaccgc gctctgtgcg ccgatttcga
     4261 cgaactgacc gaatttatcg gct
//
LOCUS       DASVTM010000369         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_884226,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000369 DASVTM010000000
VERSION     DASVTM010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884226"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VF633_10035"
     CDS             complement(<1..1042)
                     /locus_tag="VF633_10035"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX8471441.1"
                     /translation="MPDSVILGLQLLESIQNGPRLSVTTPLEEVEGVAATSDVLVLEF
                     DAFRDGRGFSLASILRERGYTGRLIAAGKVLPDQARHLRRTGFDAVELNPGADLATWK
                     RMDMAFDAAYQPAIDPTLTIWQRRAAARGVTPPPAPKDDAPEIESNLQSLADRLNTEL
                     VGADAATIVKAALDPKLGLATAAISSFGAESAALLHLIAEEDPALPVVFLETGQHFFQ
                     TLQYRSQLTAKLGLTDVRLITPDAEEKADLDARDDLWKTDADACCDLRKTRPLARATA
                     GFLALITGRKRYQAATRTALQPFEVLDGVLRINPLVNWTSDDVEDHLVAHDLPRHPLV
                     AEGYPSIGCHTCT"
     gene            complement(1035..2702)
                     /locus_tag="VF633_10040"
     CDS             complement(1035..2702)
                     /locus_tag="VF633_10040"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX8471442.1"
                     /translation="MYRYDEFDAALVRERVEEFRDQVERRASGALTEDEFKPLRLMNG
                     VYLQLHAYMLRVAVPYGVMSGGQMRQLAHIARTYDKGYGHFTTRCNIQYNWPALTDLP
                     AILSDLADVEMHAIQTSGNCIRNTTTDVFAGAADDEIEDPRPWCEVIRQWSTIHPEFS
                     FLPRKFKIAVVASEKDRAAIRTHDVGLQIVKGADGGTAFRVFVGGGQGRLPHIGQEIA
                     AAVPGHELLAYLTAIIRAWNLLGRRDNIHKARIKILVASLGIDAFREEVDKHFVTLRQ
                     EDLRIPDEELARIQSYFAPPAFADLPRQSAAYDRAVLADPDFARFSRNNVRRHRQSGY
                     ASVVVSLKPLDGVPGDITEAQMEVVADLALRYSFDEVRAAYEQNLVLPHVRLDETPAL
                     WRELLVAGLATPNADLVTDIIACPGLDYCSLANARSIPVAQALSKRLADLDWQEKVGQ
                     VRINMSGCINACGHHHVGHIGVLGVDRKGEEYYQITVGGSPDEKAALGEIVGKSLPAD
                     EVAPAIQRALDRYLEIRNEGELFIDTVRRLGADPFRDAIYAYEFADA"
     gene            complement(2702..2977)
                     /locus_tag="VF633_10045"
     CDS             complement(2702..2977)
                     /locus_tag="VF633_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEX8471443.1"
                     /translation="MKIVTANRLSDGRVIYVGADGSAVDDLSQAAVLDADQAESALAQ
                     AAARPDTFVNPYLVETDHGVFTGRDRLKESIRSAGPTVGNSLKHEIA"
     gene            complement(2974..>4314)
                     /gene="cysG"
                     /locus_tag="VF633_10050"
     CDS             complement(2974..>4314)
                     /gene="cysG"
                     /locus_tag="VF633_10050"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEX8471444.1"
                     /translation="GEPALAKLRLFQKTPAELIWFAPHGAPTGPSALQGGPTPSERLP
                     TSDDLIGARLVFIAVEDEDVAHELAALARAAGAQVNVVDRPALSDFHTPALIDRDEVV
                     IGVATGGSAPILARDVRSRIEAVLPAGLTEVAKLARDLRDTVIASVPDFMARRRFWEK
                     AFRGPAADLAAAGRTAEARREMLRLLNAASEINTGPDQGVVHIVGAGPGDPELLTLKA
                     LRVLQDADVIIHDKLVPDAILDRARRDAHRLYVGKSRSHHSVPQAQIGQLMVDEARKG
                     QRVVRLKGGDPFVFGRGGEELEAMRKAGVPVFVIPGITAAIGCAASAGIPLTHRDHAQ
                     SVTFVTAQEKSGGVAPDWQRLAGPNQTLAVYMGAGRAAETAASLIEAGRDPTTPVAIV
                     ENGSRPDERVINGSLAELGGMVARANLTGPALLFVGECAAFSAAQLDVRAELVA"
BASE COUNT          726 a         1373 c         1483 g          732 t
ORIGIN      
        1 gggtgcaggt gtggcagccg atcgagggat agccttcggc caccaggggg tggcggggca
       61 ggtcgtgggc gacgagatgg tcctcgacgt cgtcggatgt ccagttgacg agcgggttga
      121 tgcgaagcac cccgtccaga acctcgaacg gctggagcgc cgtgcgcgtc gcggcttgat
      181 agcgtttccg gccggtgatc agggctaaga accccgccgt tgcgcgagcc agcgggcggg
      241 tcttcctgag gtcacagcag gcgtcggcgt cggtcttcca caggtcatcg cgcgcatcca
      301 gatctgcctt ttcctcggca tccggtgtga tcagtcgcac atcggtcaag cccagtttcg
      361 cagtcagctg actgcggtac tgcagggtct ggaagaaatg ctggcccgtc tcgaggaaga
      421 ctacaggcag cgcagggtct tcctcggcga tcagatgcaa aagggcggcg gactcggccc
      481 cgaatgagga aatcgcagcg gttgcgaggc ccagtttggg atcgagcgcc gccttgacga
      541 tggtcgcggc gtcggctccg acaagctcgg tgttcagtcg gtcggcgagc gactgcaggt
      601 tggactcgat ctccggggcg tcatccttcg gagccggggg aggcgtaacg ccccgagccg
      661 cagcccgtcg ttgccagatc gtcagcgttg ggtcgatcgc gggttggtac gcagcgtcga
      721 acgccatatc catccgcttc cacgttgcca gatccgcgcc gggattcagc tcgaccgcgt
      781 cgaagccggt gcggcgcaga tgacgcgcct gatcaggcag gaccttcccc gcagcgatca
      841 gccgacccgt atagccccgc tctcgcagga tcgaagccag cgagaagcca cgcccgtccc
      901 ggaaggcgtc gaactccagc accagaacgt cggaggtcgc ggcgacgccc tcgacctctt
      961 ccagaggcgt tgttacgctc agacgcgggc cgttctggat ggattccagc agttgtagcc
     1021 caaggatcac tgaatcaggc atcggcgaac tcataggcgt aaatggcgtc gcggaaggga
     1081 tcggcgccga ggcggcggac ggtatcgatg aacagctcgc cctcgttgcg gatctcgaga
     1141 tagcgatcga gcgcgcgctg gatggccggg gcgacctcgt ctgcaggcag gcttttgccg
     1201 acgatctcgc ccagcgcggc cttttcgtcc ggtgagccgc cgacggtgat ctggtagtat
     1261 tcctcgccct tccgatcgac gcccagcacg ccgatatggc cgacgtggtg gtgaccgcag
     1321 gcgttgatgc agccgctcat attgattcgg acctgcccga ccttttcctg ccagtccaga
     1381 tcggccagac gcttcgacag ggcctgcgcc acagggatcg accgggcgtt ggcgaggctg
     1441 caatagtcca gccccgggca ggcgatgatg tccgtcacaa ggtcggcgtt cggcgtcgcc
     1501 agacccgcca ccagcagctc gcgccacagg gcaggcgtct cgtccagcct gacgtgtggc
     1561 aggaccaggt tctgctcata ggccgcgcgg acctcgtcga aagagtagcg aagcgccaga
     1621 tcggcgacga cctccatctg ggcctcggtg atgtcgccgg gcacgccgtc cagcggcttc
     1681 agcgatacga cgaccgaggc ataaccggac tgcctgtggc gacggacgtt gttgcggctg
     1741 aaccgggcga agtcgggatc ggccagcacc gcgcggtcgt aggcggcgga ctggcgtggc
     1801 agatcggcga acgcgggcgg ggcgaaatag gactggatgc gcgccagttc ctcatccggt
     1861 atccgcaggt cttcctgacg cagggtaacg aaatgcttgt ccacctcctc gcggaacgcg
     1921 tcgatgccga gcgaggcgac gaggatcttg atgcgcgcct tatggatgtt gtcgcgacga
     1981 cccagcaggt tccacgcccg gatgatagcg gtcagatagg ccagcaactc gtggcccgga
     2041 acagccgcgg caatctcctg accgatatgc ggtaagcggc cctgaccgcc gccgacgaag
     2101 acgcggaagg cggttccgcc atcggcgccc ttcacaatct gcaggcccac gtcgtgggtg
     2161 cggatcgcgg cgcggtcctt ttcggacgcc accacggcga tcttgaattt gcgcggcagg
     2221 aacgagaact ccggatggat ggtcgaccac tggcggatga cctcgcacca gggacgcgga
     2281 tcctcgatct cgtcgtccgc agcgcctgcg aagacgtcgg tggttgtatt gcggatgcag
     2341 tttccggacg tctggatggc atgcatctcg acgtccgcca gatcactgag gatcgccggc
     2401 agatccgtca gcgcaggcca attgtactgg atattgcagc gcgtggtgaa gtggccgtag
     2461 cccttgtcat aggtgcgcgc gatgtgggca agctgccgca tctggccgcc gctcatgacg
     2521 ccatagggga cggcgacgcg cagcatatag gcgtgcagtt gcaggtagac gccgttcatc
     2581 agccgcagcg gcttgaactc gtcctcggtc agggcgccgg acgcgcgacg ctcgacctga
     2641 tcgcggaatt cctcgacccg ttcccgcacg agggcggcgt cgaactcatc gtagcggtac
     2701 atcaggcgat ctcatgcttc aggctgttgc ccaccgtggg accggcagag cggatgctct
     2761 ccttcagccg gtcgcggccg gtgaagacgc cgtgatcggt ttcgaccaga tacgggttca
     2821 cgaatgtgtc aggtcgggcg gcggcctggg ccagagctga ctcggcctga tccgcgtcca
     2881 ggacggcggc ttgagacagg tcgtcgacgg ctgatccatc cgcgcccaca tagatcacgc
     2941 ggccgtcgct caggcggttg gcggtgacga tcttcatgcg accagctccg cgcgaacgtc
     3001 gagttgggcc gcagagaagg ccgcgcattc gccaacgaac agcagggcgg gaccggtcag
     3061 gttcgcccgc gcgaccatgc ctccgagttc ggcgagcgag ccattgatga cccgttcgtc
     3121 cggacgactg ccgttctcga cgatggctac cggggttgtg ggatcacgcc cggcttcgat
     3181 cagggaagcg gcggtctcgg ccgcacggcc tgcacccata tagacggcga gcgtctggtt
     3241 cggccctgcc agccgctgcc agtcaggagc gacgccgccc gacttttcct gcgccgtgac
     3301 gaaggtcacc gactgggcgt ggtcgcggtg cgtcagcgga ataccggccg aggccgcgca
     3361 gccgatcgcc gctgtgatgc ccgggatcac gaataccggc actcccgcct tgcgcatggc
     3421 ctccagctct tcgccgccgc ggccgaagac gaacggatca ccgcccttca gccgcacgac
     3481 gcgctgaccc ttgcgagcct cgtcgaccat cagctgtccg atctgggcct gaggcaccga
     3541 atggtgactg cggctcttgc cgacatacag ccggtgggcg tcacggcggg cgcggtcgag
     3601 gatggcgtcg ggcaccagct tgtcatggat gatgacatct gcatcctgca ggacgcgcag
     3661 cgctttcagc gtcagcagtt caggatcgcc cgggcccgcg ccgacgatat ggacgacgcc
     3721 ctgatctggc ccagtgttga tttcgctggc ggcgttcagc aggcgcagca tctcgcggcg
     3781 ggcctcggcc gtgcgaccgg cggcagccag atcagcggca gggccccgga aggccttctc
     3841 ccagaaccgg cgacgggcca tgaagtcggg cacagatgcg ataaccgtgt cgcgcaggtc
     3901 gcgggccagt ttggcgacct cggtcagccc tgcgggcagc acggcctcga tgcggctgcg
     3961 cacatcgcga gcgaggatgg gggccgagcc gccggtcgcc acgccgatca ccacttcgtc
     4021 gcggtcgatc aaggccgggg tgtggaagtc gctaagcgcc ggacggtcga cgacgttgac
     4081 ctgggctccg gctgcacgtg cgagggcggc tagttcgtgt gcaacgtctt catcttcgac
     4141 ggcgatgaac accagccgtg ctccaatcag atcgtcagag gtcggcagtc gctcagatgg
     4201 agtggggccg ccctgcagcg cgctcgggcc ggtcggcgcc ccgtgcggtg cgaaccagat
     4261 cagttcggcc ggagtcttct ggaaaagacg cagtttggcc agggcgggtt cgcc
//
LOCUS       DASVTM010000370         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_247862,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000370 DASVTM010000000
VERSION     DASVTM010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247862"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VF633_10055"
     CDS             <1..1252
                     /locus_tag="VF633_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471445.1"
                     /translation="PEFYATHVGLELGVVAAVFMIVRLIDITFDPFIGWGMDQTRTRF
                     GRYRPWMVLSTPILMLSALMMFVVVQPGAGPIYLFAWLLVLYLGFSIGTLGQLGWASV
                     LAPQYDQRSRVFGWWQVFNIIGVILILILPTVVIQLGIGTYADGVRIMGWAILIALPV
                     TMGLAMFVVPEPVNAGAAPHGGPKAYLELFKRPAVRKLLWADVLLGVAPGITGSLLFF
                     FFGQVKGYGHTEASLFMLLYFIAGLVGAPFWAWLATKIGKDKALAVASLVFAVFYVGA
                     TLVPGGSFALTAVAMFIAGLPYAAGLFLLRAMMADVGDEVRLETGVDRTGLMFSILSA
                     TTKIGHVVALIPYLILQWVGFKAVAPAAGNSEMSLVTLQILFILVPGLLLAGAAWVLK
                     GYPLTPARHDDIRKALEARDGAAA"
     gene            1249..>1957
                     /gene="mazG"
                     /locus_tag="VF633_10060"
     CDS             1249..>1957
                     /gene="mazG"
                     /locus_tag="VF633_10060"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX8471446.1"
                     /translation="MTPIERLQGIMARLRDPDGGCPWDLEQTFATIAPYTIEEAYEVA
                     DAVERGDMTELKSELGDLLFQTVFQTRIAEEAGLFTFDDVANAMSDKLERRHPHVFGD
                     EAAKADAVAQKARWEDIKADERAAKAQHGVLDDVPVGLPALARAAKLTKRAARVGFDW
                     PSTDEVFDKLFEEVEELRVEIAAGDLDKARDEMGDLLFVVANLARKLQVEPEDALRGA
                     NAKFVRRFGFIEAELAKD"
BASE COUNT          290 a          615 c          682 g          370 t
ORIGIN      
        1 gccggagttc tacgccaccc atgtgggtct ggagctgggc gtggtggctg ctgtattcat
       61 gatcgtgcgg ctgatcgaca ttacattcga tcccttcatc ggctggggca tggatcagac
      121 gcggacgcgg ttcggccgct atcgcccgtg gatggtgttg tcgacgccga tcctgatgct
      181 gtccgccctg atgatgttcg tcgtggtcca gccgggcgcc gggcccatct atctgttcgc
      241 atggctgctg gtgctctacc tcggcttctc cataggaact ctgggccagc tcggttgggc
      301 gtcggtgctc gcgccgcagt acgaccagcg cagccgggtc ttcggctggt ggcaggtgtt
      361 caacatcatc ggcgtgatcc tgatcctgat cctgccgacg gtggtgatcc agctgggcat
      421 cggcacctat gcggacgggg tgcggatcat gggctgggcc atcctgatcg ccctgcccgt
      481 caccatgggc ctggccatgt tcgtggtgcc cgagccggtc aacgccgggg ccgcgcccca
      541 tggcggaccc aaggcgtatc tggagctatt caagcgccct gcggtgcgca agctgctgtg
      601 ggctgacgtt ctgctgggcg tcgcgccggg catcaccggg tcgctgctgt tcttcttctt
      661 cggtcaggtg aagggctacg ggcacaccga ggcctcgctg ttcatgctgc tgtatttcat
      721 cgccggactg gtcggggcgc cgttctgggc ctggctggcg acgaagatcg gcaaggacaa
      781 ggccctcgct gtcgccagtc tggtcttcgc cgtcttctat gtgggcgcga ccctggtccc
      841 gggtggcagc ttcgccctga ccgccgtggc catgttcatc gccggactgc cctatgcggc
      901 gggactgttc ctgctgcgcg caatgatggc cgacgtgggc gacgaggtgc ggttggagac
      961 cggcgtggac cggaccggcc tgatgttctc gatcctgtcg gcgaccacca agatcgggca
     1021 cgtcgtggcc ctgataccgt atctgatcct gcagtgggtg ggcttcaagg cggtagcgcc
     1081 tgccgcaggg aacagcgaga tgtcgcttgt gacactgcag atcctgttca tcctcgtgcc
     1141 cggactgttg ctggccggag ccgcgtgggt gctgaagggc tatcccctga cccccgcgcg
     1201 gcatgacgat atccgcaagg cgctggaagc gcgtgatggg gctgctgcat gaccccgatc
     1261 gaacgcctgc agggcatcat ggcgcggttg cgggacccgg acggcggctg tccgtgggac
     1321 ctggagcaga ccttcgccac catcgcgccc tacacgatcg aagaggccta tgaggtcgcc
     1381 gatgcggtcg agcggggcga catgaccgag ctcaagtccg agctcggcga cctgctgttc
     1441 cagaccgtgt tccagacgcg aatcgccgag gaggccgggc tgttcacctt cgacgacgtg
     1501 gccaacgcca tgtccgacaa gctggaacgg cggcatccgc atgtctttgg ggacgaggcg
     1561 gcgaaggccg acgccgtggc ccagaaggcg cgctgggaag acatcaaggc cgacgaacgg
     1621 gccgccaagg ctcagcatgg ggtgctggac gatgttccgg tcggcctgcc tgcgctggcc
     1681 cgcgccgcca agctgaccaa gcgcgcggcc cgggtcgggt tcgactggcc gtccaccgac
     1741 gaggtgttcg acaagctgtt cgaggaggtc gaggaactcc gcgttgagat cgccgccgga
     1801 gacctcgaca aggcccgcga cgagatgggc gacctgctgt tcgtcgtcgc caacctggcc
     1861 cgcaagctgc aggtcgaacc cgaggacgcc ctgcgcggcg ccaacgccaa attcgtccgg
     1921 cgtttcggct tcatcgaggc ggaactggcg aaggacg
//
LOCUS       DASVTM010000371         9867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_991109,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000371 DASVTM010000000
VERSION     DASVTM010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991109"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF633_10065"
     CDS             <1..211
                     /locus_tag="VF633_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471447.1"
                     /translation="DNSPEGSSGAGTTTGADGDPVRSGPAAGQVWGGLAVWLMARAVW
                     GKAAALPVKAAAPRRVRRFQIIRVW"
     gene            complement(274..795)
                     /locus_tag="VF633_10070"
     CDS             complement(274..795)
                     /locus_tag="VF633_10070"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8471448.1"
                     /translation="MFHSGTQTLIDHWTALPEARRVPARADFQPMTFGPLTPKLFIVE
                     REGARMRLAGGWVERLHGRVLRGADWLSVWSEASRPMVTAAMIQAFREARPVVLSADA
                     AGLAGVIEIVIAPLRNSDGLADRLVGLYQAVSPLDRRADDVGPLTARLSVGVGGTSRA
                     PLSLAAVDGRRIA"
     gene            952..1971
                     /locus_tag="VF633_10075"
     CDS             952..1971
                     /locus_tag="VF633_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471449.1"
                     /translation="MTSLGAQPMRVSIKTWLPTIVGLMAFGAVGGAAVAQCGSCQPPP
                     QPSCCQPPPAPPPPPPPTSCCQAPPPPSYPGGNGNFNVNVNVNVNANANANANANANS
                     LINARGRGDTYVNNNGGAYFTVDQPYPTVIEGLNVQGAAVAQVIRTPFSASRWMASRV
                     VIQAVCIDDRDIPHPASQVQPGREVANGYEGELYRCIAGTRLQVTWADYVDQISFDHG
                     QTMSCRKGESLWYGQSAAVAEYAASYADAYAQSWAAAQGGVVQAGEGGPLGGGVMECR
                     PQRPERDCNERSLLRRYGAGVKILTMWREETYTEYREETVQAAGISISGASMTLDGGV
                     GGRVF"
     gene            complement(2103..5033)
                     /locus_tag="VF633_10080"
     CDS             complement(2103..5033)
                     /locus_tag="VF633_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8471450.1"
                     /translation="MISPRRLLLLAASSAAILAGATLPAIAQTAPAATSASSSVAATP
                     SDPWAQAISDIPADPAVRFGVLPNGMRYAIRHNATPPHQAALRLRIDAGSLSERDDQQ
                     GLAHFLEHMAFNGSTHIAEGDMTKNLERLGMSFGGDTNAETSFDQTTYKLNLPNTSDE
                     VVDASLFSLREVSSELLFAAEAVERERGVIVGEERTRDDPGYRSLKALFPFIAPGQRL
                     GDRLPIGDLDIIRTAPRDRFVDFYRAYYRPERATLIAVGDFDVDAMEAKIKARFGDWT
                     NPSANGPDPDLGQIKPRQPETLIHSEPGAQSSAQIFWTVPHDASPDTVAERRKSLVRS
                     LGMSVLNRRFSNIARLPNPPFLGGGAGYQSLYNALDVGTLSVSFNPGGWKPAIETAEQ
                     EQRRLVQYGVGQSELDREITEYRTAIQAAAASAATRQTTGLAEGLAGAANDNEVFNTP
                     ADDLKQFNEAVASITVDEVNAATREAFSGNGPLVFITSPVAIEGGEAAVTAALEASRQ
                     TPVAAPVIVADTKWPYDSFGTPAQPSSQSEIADLGATLVTFPNGVRLTIKPTTFADKQ
                     ILVSVRAGNGYLDLPTDRVTPVAWAAASELVEGGLGKLTAAQLEQVTAGNVVGASFGP
                     DQNAFQYGGATRPEDLPLQMQLLAAYFTDPAWRPEPFARIQGALPQNLAQARAVPAGA
                     FSLDATGLLYGGDPRFGVPTEPEVAAARLDDMKTLVTESVSRGPIEIIMVGDVTVADA
                     IASVGATFGALPTRAAPVTPSSKALDVKFPAPTPRPLEFHHTGLPTQSLGYVAWPTID
                     SIGDRKPGRIVSLLSKVLQLRVTEELREKQGVAYSPGAGATSSIVFPGYGYVFTQIET
                     PPEALPGFFESVDTIAAGLRDTPVTDDELGRARLSTIEGLRRSQANNGYWISALRGVQ
                     TDPAQLAAVRTAISDLEAVTPADLQKAAQTYLLPDRAWRAQVTAAAATAPAAETAPAP
                     TPAQ"
     gene            complement(5181..6428)
                     /gene="hfaD"
                     /locus_tag="VF633_10085"
     CDS             complement(5181..6428)
                     /gene="hfaD"
                     /locus_tag="VF633_10085"
                     /inference="COORDINATES: protein motif:HMM:NF037936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchor protein HfaD"
                     /protein_id="HEX8471451.1"
                     /translation="MARLAPIQIAGILAATAMCVAAATEAVAQEGQGLVLNNQLQLGD
                     VISGQTLNVEGATDQVTVSNAATGNSLSGATQGVSLDVRNVQTMNGNARATTEISTTG
                     DTDAVINATTQATGNSLAGGAYGGDFAIDSDQRVGPSEITASTAIRGGAARLLNGANV
                     TAAATGNAAALAGNVGMISGAVHQVSDASVRAENLAETQYIPAAAVFGAQAINNAVAV
                     NGTDQSGQSIYIGQRSTGDAVTANTSANAGNAWDLTGASVATANQAVIANQGGSVVAR
                     TQQENVSQVASTAVTTSYDYGRAVASAEGTGNAASVGNNDKYLELDNSQLNSGGVEVQ
                     ASFAGAQGYDVYVSANAAGNSVTGYACAECQATLNATNNQINDGNVSASANTTITGAN
                     RNVITGSNAVGNAATFYVSRPGQ"
     gene            complement(6355..7365)
                     /gene="hfaB"
                     /locus_tag="VF633_10090"
     CDS             complement(6355..7365)
                     /gene="hfaB"
                     /locus_tag="VF633_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaB"
                     /protein_id="HEX8471452.1"
                     /translation="MINRKLSTRTRLVGMACAAAALLTGCVSPVAGSNGMYATPIGNA
                     PVTANPTPYSTALYCMADYARRYNLPSPRIAVGRISDYTGSVSSDGGRQITQGASLMA
                     YSALAKSGARMVERYDTSISELELRYANNKLIGEEGAAPESNSYRRILAGQVPGSDFY
                     LVGGVTEVNYNIRSSGVDIGQGETNSVKPGSVLVQGKVFVMNVAIDLRLVQTTSMEVV
                     DVISYQKQIIGREVSAGVFDFLGGNVIDISAGTGGMEPVQLAVRSLIERGTLEMMANL
                     YGAPGPQVCLDAANDPLGTVGPTGGYYPAYANTDSNNGQTRADPNRWHSGRDGDVRSS
                     RY"
     gene            complement(7358..7816)
                     /gene="hfaA"
                     /locus_tag="VF633_10095"
     CDS             complement(7358..7816)
                     /gene="hfaA"
                     /locus_tag="VF633_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaA"
                     /protein_id="HEX8471453.1"
                     /translation="MTLFRTIQKAGFASLFGTACLMGMTAPALAQTTGSGGAASFQAG
                     YGNARYAVTQPSTGSTRDANGNRLIVDGIIQSGASAYSRASAGAASSYAGAGSSGNGG
                     TTIGGATAIGNNLNVVVQGNHNTVIVNSRQTNTGNINAGTVLNGTLDLDD"
     gene            7934..8701
                     /locus_tag="VF633_10100"
     CDS             7934..8701
                     /locus_tag="VF633_10100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8471454.1"
                     /translation="MTAGQDRFETTPGGATEAPPAGPPIWEPQSPPPGDDGREKIFNA
                     PLLPILIALSMPALFYLQQSLPDEGLRWAFFPDTLRTGGWWPGVLTSMTLHGGWTHAL
                     MNAGFAVAFGPPIARLFAGAKGGFIFFAYYIVCGLIGTLGYGLIHLGSDAPMVGASGA
                     VTGLLGGAIRLLGTDGRVRPLTDRRVLTTAVVILILNAITGLIGFAPGADGAGIAWEA
                     HAFGFLTGLLLIGPLARWFGKPPEGFASSSGLGDPRS"
     gene            8795..9223
                     /locus_tag="VF633_10105"
     CDS             8795..9223
                     /locus_tag="VF633_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8471455.1"
                     /translation="MLVAEILKAKGGAVFTIAPDTTVSQACGQLDSRRVGALIVCDGD
                     RVAGVFSERDVVKAVAADGVAALDRPVSTYMTAEVIFAKPSESVAILMGRMTDRRIRH
                     LPVLHESRLAGVISIGDVVKCQIAEATQEAESLRTYISAG"
BASE COUNT         1658 a         3039 c         3363 g         1807 t
ORIGIN      
        1 agacaatagc cccgaaggct cttccggcgc agggacgacc acgggtgcgg acggcgatcc
       61 ggtcaggtcg ggacctgcgg cgggccaggt ctggggcggc ctggcggtct ggctcatggc
      121 ccgggctgta tggggcaagg cggcagcgct gcctgtgaag gctgcggcgc cgagaagggt
      181 gcggcggttc cagatcatcc gtgtttggtg acaccggtat ccagagcagg caagcccctt
      241 gctgatccgc ccctgaacgc cgcgggttcg ggttcaggcg atccggcgac cgtccacggc
      301 cgcgaggctt agaggagcgc ggcttgtccc gcccacgccg acggagaggc gtgccgtcag
      361 aggtccgaca tcgtccgcgc gccggtcaag cggactcacg gcctgataca gaccgaccaa
      421 tcgatcggcg agcccatcgc tgttccgcaa tggcgcgatg acgatctcga tcacgcccgc
      481 cagtccggcg gcgtcggcgg aaaggacgac cggccgcgcc tcccggaagg cctgaatcat
      541 tgcagcggtg accatcggcc ggctggcctc gctccacacc gacagccagt cggcgccgcg
      601 gagcacgcgg ccatgcaaac gttcgaccca gccgcccgcc agccgcatcc gtgcgccctc
      661 tcgttcaacg atgaacaact ttggcgtgag cggtccgaac gtcatcggct ggaagtcagc
      721 acgcgcagga acccgccgcg cctccggcag ggcggtccag tggtcgatca aggtctgggt
      781 accggaatgg aacatgacgc ggcctttcga aggccctgcc gcaagacggg cgccgggaga
      841 ccctccagcc ggaatgactt gccgccagaa cgacttggct aacggaaacg caagggtttt
      901 gccttcaagt gggctctgga ccgttccgca tgtcgtggag ggtccgcgtg aatgacttct
      961 ctgggagccc agcccatgcg ggtgagcatc aagacctggc tgccgacgat cgtcggtctg
     1021 atggcgttcg gcgccgtcgg cggtgcggct gtagcccagt gcggctcgtg ccagccgccg
     1081 cctcagccct cgtgctgcca gcccccgcct gcgcctccac cgccgcctcc ccccacgtcc
     1141 tgctgccaag cgccgccgcc cccgagctat ccgggcggca acggcaactt caatgtgaac
     1201 gtcaacgtca acgtcaatgc gaacgccaat gcgaacgcga acgcgaacgc caactcgctg
     1261 attaacgccc gcggtcgcgg cgacacctat gtgaacaaca acggcggcgc ctacttcacc
     1321 gtcgaccagc cctatcctac ggtcatcgaa ggtctgaacg tccagggcgc agctgtcgcc
     1381 caggtcatcc gcactccatt cagcgcctcg cgctggatgg ccagtcgggt ggtgatccag
     1441 gcggtctgta tcgacgatcg tgacattccc caccccgcct cgcaggtcca gcctggccgt
     1501 gaagtcgcca acggttatga gggcgagctc tatcgctgca tcgccgggac ccgtcttcag
     1561 gtcacctggg ccgactatgt cgaccagatc agcttcgatc atggccagac catgagctgc
     1621 aggaagggcg agagcctctg gtacggccag agcgctgcgg tggccgaata cgccgccagc
     1681 tacgccgatg cctacgcgca atcgtgggcg gcggcccagg gcggagtcgt tcaggctgga
     1741 gaaggcggac cgctgggcgg aggcgtcatg gaatgccggc cgcagcgtcc cgaacgcgac
     1801 tgcaacgagc gctcgctact tcgccgctac ggcgccggcg tcaagatcct gaccatgtgg
     1861 cgtgaagaga cctataccga gtatcgcgaa gagacggttc aggctgcggg gatctcgatc
     1921 tccggcgcct cgatgacgct ggacggcggc gtcggaggcc gggtgttctg atctgaggac
     1981 ggattgaacg ggccgttcac gctgcggaca ggccaggccc gatactttgc agttgtcggt
     2041 tcatcgccgg cgatcctgag aaaccaactg gccccggtgc gaaagcaccg gggccttttt
     2101 cgttactgcg caggcgttgg ggccggagcc gtctctgcgg ctggcgccgt tgccgctgcg
     2161 gcggtcacct gagcgcgcca tgcccggtcg ggaagcaggt aggtctgtgc ggccttctgc
     2221 agatcggccg gggtgactgc ttcgagatcg gaaatcgccg tacggacggc cgccagctgg
     2281 gctggatcgg tctggacgcc gcgcagggcg ctgatccagt aaccattgtt ggcctggctg
     2341 cgacggaggc cttcgatggt cgacaggcga gcccggccaa gttcatcgtc cgtgaccggc
     2401 gtatcgcgca ggcctgctgc gatggtgtcc acgctctcga agaagccggg cagggcctca
     2461 ggcggcgtct cgatctgggt gaacacgtag ccgtagccgg gaaatacgat cgatgacgtg
     2521 gcgcccgcgc ccggagaata ggccactccc tgcttctcgc ggagttcttc cgtcacccgc
     2581 aactggagga ccttcgacag caggctgacg atccggcccg gcttgcgatc accgatggag
     2641 tcgatcgtcg gccaggcaac gtagccgagc gattgggtgg gcaggcccgt gtggtggaac
     2701 tcgagcggcc tgggcgtagg ggccgggaac ttgacgtcca aggccttgga cgacggcgtg
     2761 accggagcag cgcgtgttgg cagggcgccg aaggtcgcgc ctacggaggc gatagcgtct
     2821 gccacggtga catcacccac catgatgatc tcgattggac cccgcgagac cgactcggtc
     2881 accagcgtct tcatgtcgtc gagacgcgcg gcggcgacct caggttcggt tggcacgccg
     2941 aaacggggat ctccgccata gagcaggccg gtcgcatcga gggagaaggc gccggcaggc
     3001 acggcccggg cctgggccag gttttgcggc agtgccccct gaatccttgc gaagggctcg
     3061 ggccgccagg cgggatcggt gaagtaggct gccaacagct gcatttgcag cggcaggtct
     3121 tcgggacggg tggcgccgcc gtactggaag gcgttctggt ccgggccgaa gctggcgccg
     3181 acgacattgc ctgccgtaac ctgttcaagc tgcgctgcgg tcagcttgcc gaggccgcct
     3241 tcgaccaatt cggatgcggc ggcccaggcg acaggggtta cgcggtcggt cggcaagtcg
     3301 aggtagccgt ttcctgcgcg caccgagacg aggatctgct tgtccgcaaa ggtcgtcggc
     3361 ttgatcgtca gccgaacgcc gttggggaag gtgacgagtg tcgcgccgag atcggcgatc
     3421 tcgctttgac tcgatggctg cgcaggcgtg ccgaagctgt cgtaaggcca tttggtgtca
     3481 gcgacgatga ccggggcagc caccggagtc tggcgcgacg cctcgagagc cgccgtgacc
     3541 gcggcttctc cgccttcgat ggcgacaggg ctggtgatga acaccagcgg accgttaccg
     3601 ctgaaggctt cacgtgtggc cgcgttgacc tcatcgacgg tgatcgaggc gacagcttcg
     3661 ttgaactgtt tcagatcgtc cgccggggtg ttgaacacct cgttgtcgtt cgcggcgccg
     3721 gccaacccct cggcaaggcc ggtggtctgg cgcgtggctg cgctggccgc agcggcctga
     3781 atggccgtgc ggtattcggt gatctcgcgg tcgagctcag actggccgac cccgtactgg
     3841 acgagcctgc gctgttcctg ctcagccgtc tcgatggcgg gcttccatcc gcccggattg
     3901 aagctgaccg acagcgtacc gacgtccagc gcattgtaga gcgactgata gccagccccg
     3961 ccgccgagga agggcggatt cggcaaacgt gcgatattgc tgaagcggcg gttcaggacc
     4021 gacatgccca ggctgcgaac cagcgacttc cgacgctctg cgacggtgtc cggcgaagca
     4081 tcgtggggga cggtccagaa gatctgggcc gaagactggg cgcccggctc actgtggatc
     4141 agggtttcgg gctgtcgagg tttgatctgg ccgagatccg gatcggggcc atttgcactc
     4201 ggattggtcc agtcgccgaa acgagccttg atcttcgcct ccatcgcatc gacgtcgaag
     4261 tcgccgactg cgatgagggt cgcgcgttcg gggcggtagt aggcgcggta gaagtcgacg
     4321 aaccggtcgc gcggagcggt ccggatgata tccaggtccc cgatgggaag acgatctccg
     4381 agccgctgac cgggcgcgat gaaaggaaac aaggctttga gcgagcgata gccgggatcg
     4441 tcgcgcgtcc gttcctcgcc gacgataacg ccgcgctcgc gctccacggc ttcggcagcg
     4501 aacaagagtt cggacgaaac ctcgcgcagg gagaacaggc tggcgtcgac gacctcatcc
     4561 gaagtgttcg gcaggttcaa cttgtaggtg gtctggtcga agctggtctc ggcgttggta
     4621 tcgccgccga acgacatacc cagccgctcg aggttcttcg tcatgtcgcc ttcggcgata
     4681 tgggtcgatc cgttgaaggc catgtgctcg aggaagtggg ccagaccctg ctggtcgtcc
     4741 cgctcggaca gcgacccggc gtcgatgcgc aggcgcaggg cggcctgatg cggcggagtg
     4801 gcgttgtgcc ggatagcgta gcgcatgccg ttgggcagga cgccgaagcg cacggctgga
     4861 tccgccggga tgtcgctgat ggcctgcgcc cagggatcgg acggtgtcgc tgcgacggat
     4921 gaggaagccg aggtcgctgc tggagcagtt tgcgcaatgg cgggcagggt agcgcccgcc
     4981 agaatcgcag cgctcgaggc ggcgagcaac aacaggcggc gaggggaaat catgcagacg
     5041 ctccgaatga gggaatcgtc cgagaccgcc ttagccgacc ggacgcacca cccatacggg
     5101 aggaacgcgc ccgctcaagc gatttcgtga ggggaggatc gactggcctg tggggatcag
     5161 gccagtccgg ggtcggagca ttactgaccc gggcgcgaaa cgtagaaggt tgcggcgttg
     5221 ccgacggcgt tagagccggt gatcacgttg cggttggcgc cggtgatcgt tgtgttcgcg
     5281 ctagcgctga cgtttccgtc gttgatctgg ttgttggtgg cgttcagcgt cgcctggcat
     5341 tccgcgcagg catagcccgt caccgagttg cccgcggcgt tggctgagac atagacatcg
     5401 taaccctgcg cgcctgcgaa gctggcctgg acctcgaccc cacccgagtt gagttggctg
     5461 ttatccagct cgaggtattt gtcgttgttg ccgactgagg ccgcattacc cgttccctcg
     5521 gcggaggcca cggcgcgccc gtaatcatat gaagtcgtga cggccgtcga tgcaacctga
     5581 gagacgtttt cctgctgggt gcgggcgacg accgatccgc cctggttggc gatcacggcc
     5641 tggttggcgg tggcgacgga tgcgccggtc agatcccagg cgttgcctgc gttggcgctg
     5701 gtgttggccg tgaccgcgtc gccggttgag cgctggccga tgtagatcga ctggcctgac
     5761 tgatcggtcc cgttgacggc aacggcgttg ttgatcgctt gggcgccgaa tacggctgca
     5821 gcagggatgt actgggtttc ggcgaggttc tcggcccgaa ccgaggcgtc cgaaacctga
     5881 tgcacagcgc cggagatcat gccgacattg ccggcgagag cggcagcatt gccggtcgct
     5941 gcagcggtga cattggcgcc gttcagcagg cgcgccgcgc cgccccggat ggccgtcgag
     6001 gcggtgatct cggatggacc gacgcgctgg tcggaatcga tggcgaagtc accgccgtag
     6061 gctccaccgg caagagagtt gccggtggcc tgtgtcgtgg cgttgatcac cgcgtcggta
     6121 tcgccagttg tcgagatttc agtggtggcg cgggcgttgc cgttcatcgt ctggacgttg
     6181 cgcacgtcaa gggacacgcc ctgcgtcgcg ccggacaggc tgttgcccgt ggcggcgttg
     6241 gacaccgtca cctgatcggt cgccccctcg acattgaggg tctggccgct gatgacgtcg
     6301 ccgagctgca gctggttgtt gaggacgagg ccttgcccct cctgcgcgac agcctcagta
     6361 gcggctgcta cgcacatcgc cgtcgcggcc agaatgccag cgatttggat cggcgcgagt
     6421 ctggccattg ttgctgtccg tgtttgcata ggcgggataa tagccgccgg tcgggcctac
     6481 cgtgcccaga ggatcgttgg cggcgtcgag acagacctgg ggtccgggcg cgccgtagag
     6541 gttcgccatc atttccagcg tgccacgctc gatcagcgag cggacggcca gttgaaccgg
     6601 ctccatcccg ccggtgccgg ccgagatgtc gatgacgttg ccgccgagga agtcgaacac
     6661 gccggcggag acttcgcggc cgatgatctg cttctggtag gaaatcacat cgaccacctc
     6721 catcgaggtc gtctgcacca gacgcaggtc gatggcgacg ttcatgacga acaccttgcc
     6781 ctgcaccagc accgagcccg gcttgaccga attggtctcg ccctgaccga tatccacgcc
     6841 ggacgagcgg atgttgtagt tcacctcggt cacgccgccg accagataga agtcagagcc
     6901 gggcacctga cccgcgagga tgcggcggta gctgttgctt tcaggcgctg cgccttcttc
     6961 gccgatcagc ttgttgttgg cgtagcgaag ctctagctcc gagatcgagg tgtcgtaacg
     7021 ctcgaccatc cgcgcgccgg acttggccag agccgagtag gccatcagcg aagcgccctg
     7081 cgtgatctgg cgaccgccgt cggacgagac cgaaccggtg tagtcgctga tccgaccgac
     7141 cgcgatgcgc ggcgagggca ggttgtagcg tcgggcgtag tccgccatgc agtacaacgc
     7201 tgtggaatag ggcgtcgggt tggcggtcac cggcgcgttt ccgatcgggg tggcgtacat
     7261 cccgttcgac ccggcgaccg gactgacgca gccagtcagc aatgcggctg cggcgcaggc
     7321 cattcccacc agacgcgtgc gcgtcgaaag tttgcgatta atcatcgagg tctagcgtcc
     7381 cgttcaacac cgtcccggcg ttgatgttgc cggtgttcgt ctgtctggag ttgacgatca
     7441 cggtgttatg gttgccctga accacgacgt tcaggttgtt gccgatggcg gtcgcgccgc
     7501 cgatggtcgt gccgccgttg ccggatgagc ctgctccggc atagctggag gctgcgcctg
     7561 cgctggctct tgaataggcc gaagcgccgc tctggatgat gccgtcgacg atcagacgat
     7621 tgccgttggc gtcacgggtt gaaccggtcg aaggctgcgt caccgcataa cgggcgttgc
     7681 cgtacccggc ctggaagctg gcggcgccgc cggacccggt cgtctgggcg agggcagggg
     7741 cggtcatacc catcaggcat gccgttccga acagggacgc aaaaccggcc ttctggatcg
     7801 tccggaatag ggtcatcgcg ggtctccgca cgcgtacttg gttgatgggt cgtcaagcgc
     7861 cccccgtgcc agaatgggat atggttatta agaagcgctt gagattgtgg aggtctccgc
     7921 gcattccgtt cgaatgaccg ctggccagga cagattcgaa accactccgg gaggcgccac
     7981 ggaggctccg cccgctggtc cgccgatctg ggagccgcaa tctccgcccc ccggggatga
     8041 cggacgggag aagatattca acgcgcccct tctgccgatc ctgatcgcgc tgtcgatgcc
     8101 tgcgctattc tacctccagc agagcctgcc cgacgaaggt ctgcgttggg cgttcttccc
     8161 cgataccttg cggacgggtg gctggtggcc cggagtgttg actagcatga cgcttcacgg
     8221 cggctggacg catgcgttga tgaacgcggg ttttgctgtc gccttcggtc cgcccatcgc
     8281 gcgactgttc gcgggagcga agggcggatt catctttttc gcctattata ttgtatgcgg
     8341 cctgatcggg acgctcggct acggtctcat ccatctgggt agcgacgccc cgatggtggg
     8401 cgcgtcaggc gcggtgaccg gcctgctagg cggcgcgatc cgcctgctgg ggaccgacgg
     8461 tcgcgtccgc ccgctgacgg atcgtcgagt cctgaccacg gctgtcgtga tcctgatcct
     8521 caatgctata actggcctga tcggctttgc tcccggcgcc gacggggcag gcattgcctg
     8581 ggaagcccat gctttcggct ttctgacagg tctgctgctg atcggtccgt tggcgcggtg
     8641 gttcggaaaa ccacctgaag ggtttgcctc ttcgtccggt ctgggcgatc ctcggtcatg
     8701 aagcggcccg acaggaggcc cgatcacgag cccttgcccg gatcgcaagc tctggcggcg
     8761 tcgttctcta caacgataga gaaggaaacg cctgatgctc gtcgccgaaa tcctcaaggc
     8821 caaaggaggt gcggtcttca cgatcgcgcc tgatacgacg gtttcccaag cctgcggcca
     8881 gcttgatagc cgaagggtcg gcgccctgat cgtctgtgac ggcgatcggg tggccggcgt
     8941 cttctccgaa agggatgtcg tcaaggccgt cgccgccgat ggggtcgctg ctctggatcg
     9001 acccgtatcc acctatatga ccgccgaggt gatcttcgcc aaaccctctg aaagcgtcgc
     9061 catactgatg gggcggatga cggaccgacg gatccggcat ttgccggtgc tgcacgagag
     9121 ccggctcgcc ggagtgatct ccatcggtga cgtcgtcaaa tgccagatcg ccgaggcgac
     9181 gcaggaagcc gagagtctgc gaacctatat ctcggccgga tagcggctga cccacgagac
     9241 cgtcactgtg gaggccggtc atccggaagt gaccgccgtc cgctgacggt gcatggcgag
     9301 aaacatcggc tgccttgaaa atgccgcttg ctcccacgtc ggctgctacg tatatcgccg
     9361 cctcgcccgg agacgggccg gccgccggag cagtttgaga cgaaagtctt ttggttccac
     9421 cggagacgag aagttcaaaa aacttcttgc ttctgtaacg cagtcgacat agtctccgcg
     9481 ctccaatcga atcgttcggt aacgaaataa ggggcaagcc tctcgcttct cctgacggtt
     9541 ttttgcggcc cgaaggcttt ggtcttcact gcaaaacggc ttgaaaaagc ccggttgaca
     9601 cggagaacgg ccttccttag agagccgcct ccgccgaacc ttccggggtt cggaagtcga
     9661 cctcaagggg ttggcgacgg tgttaaaatc cttcttagga aggttgttga cacggacttc
     9721 cgaggcgact aaatcgccgc ctccgccgct caccggcgta aaacagtggg gccgcctgga
     9781 gcggtttacg gtctttgaca ttgttgattt ggaaagagaa acgcaggcgg cggtgttctt
     9841 gcggtaggtt aatgacctgc cacgatt
//
LOCUS       DASVTM010000372         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_89858, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000372 DASVTM010000000
VERSION     DASVTM010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89858"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VF633_10110"
     CDS             <1..257
                     /locus_tag="VF633_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HEX8471456.1"
                     /translation="PNNGGAVVTAGNLIFIAAATDGLIRAIDINTGKTVWSDVLPGGG
                     QTTPMTYEINGRQYLVIAPGGHHFMETPITDEVIAYALPT"
     gene            complement(323..1225)
                     /locus_tag="VF633_10115"
     CDS             complement(323..1225)
                     /locus_tag="VF633_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8471457.1"
                     /translation="MTEDLSAPLIAGVELGGTKIVCLLASGPDDVRAEVRLPTTSSDE
                     TLGAIAEVLARWKAEQGFAALGIGSFGPLELDDRASNYGAIVSTPKPGWNNADLTVLG
                     RGLDVPVAIDTDVNGTAFAEGLWGAAQGLRSFAYVTVGTGIGVGSIVNGQSVKGLGHS
                     EAGHLRVGRIAGDVWPGSCPYHGDCVEGLAAGPALLARTGKRGETLSGDDPAWEMVVR
                     ALGGLLHNLVLTTAPERILIGGGIFGGNDHLFPRLRSALVESLAGYATGDRIAAMIDD
                     FVTPPGLGGMAGPLGAIVLGRKAL"
     gene            1369..2076
                     /gene="rutB"
                     /locus_tag="VF633_10120"
     CDS             1369..2076
                     /gene="rutB"
                     /locus_tag="VF633_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein B"
                     /protein_id="HEX8471458.1"
                     /translation="MAAKITLPARPESLEIDPAITAVIVIDMQNAYASPGGYLDLAGF
                     DISGAAKVITNIKGVLEVARGAGVQVIYFQNGWDPDYVEAGGPGSPNFHKSNALKTMR
                     ARPELHGQLLARGTWDYELVDELKPQPGDISLHKTRYSGFFNSQLDSVLRSRGIRNLV
                     FVGIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPDFVQQASLYNIEKFFGWVSSVA
                     DFKGVVGQIAPATQQET"
     gene            2078..2467
                     /gene="rutC"
                     /locus_tag="VF633_10125"
     CDS             2078..2467
                     /gene="rutC"
                     /locus_tag="VF633_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HEX8471459.1"
                     /translation="MPKTVITPPGTVTPIAPFSPGTLADGVVYVSGTLAFDKDNNVAC
                     VGDAEGQTRIVLETIKSVIETAGGTMDDVTMNHIFLKDWADYPALNKVYAEYFPGEKP
                     ARFCIQCGLVRPDFLVEIATIAHIGKT"
     gene            2464..>2787
                     /locus_tag="VF633_10130"
     CDS             2464..>2787
                     /locus_tag="VF633_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEX8471460.1"
                     /translation="MNPLATLLIKAVVSAILIVAASEVARRQPGVGALIASLPLVSVL
                     GMIWLWSSKPDAENMAQHAQATFWYVLPSLPMFLVIPALLRRGVPFWPALGLGCALTV
                     GLYLAM"
BASE COUNT          486 a         1053 c          797 g          451 t
ORIGIN      
        1 cgccgaacaa cggtggtgcg gtcgtcacgg ccggcaacct gatcttcatc gccgccgcga
       61 ccgatggcct gatccgcgcc atcgacatca acaccggcaa gacggtctgg tccgacgtcc
      121 tgcccggtgg gggtcagacg acgccgatga cctacgagat caatggccgc caatatctgg
      181 tcatcgctcc cggaggccac cacttcatgg agaccccgat caccgacgaa gtgatcgcct
      241 acgccctgcc gacctgaggc acggcatacg acagaatgcg ggacggccgc cgggacatcc
      301 cgggcggccg cttcgctgcc tgctaaagcg ccttgcggcc cagtacgatc gctcccaagg
      361 gtccggccat cccgccgagc cccggcggcg tgacgaaatc gtcgatcatc gctgcgatcc
      421 tgtcgcccgt cgcatagccc gcaaggctct cgaccagcgc cgacctcaga cgcgggaaca
      481 ggtggtcatt gccgccgaag atgccgccgc cgatcagtat ccgctccgga gccgtggtca
      541 gcaccagatt gtgcagcagc ccgcccagcg cccgcaccac catttcccag gccggatcgt
      601 cacccgacag ggtctcgccg cgcttcccgg tccgcgccag cagcgccggt cccgccgcca
      661 gcccctcgac gcaatcgccg tgatacggac agctccccgg ccagacatcg cccgcgatgc
      721 gcccgacgcg caaatgcccc gcctccgaat ggccgagccc tttcaccgac tggccgttca
      781 cgatcgaccc caccccgatc cccgtgccga cagtgacata ggcgaacgac cgcagcccct
      841 gagccgcacc ccaaagcccc tcggcgaagg ccgtgccgtt gacgtcggta tcgatcgcca
      901 caggcacatc gagaccccgc cccagcaccg tcaggtctgc attgttccag cccggtttgg
      961 gcgtcgagac gatcgcgcca taattcgacg cccgatcatc cagctccagc ggcccgaaac
     1021 tgccgatccc cagcgccgca aacccttgct ccgccttcca gcgagctaac acttcggcga
     1081 tcgctcccag cgtctcgtcc gacgaggtcg tcggcagccg cacctcagcg cgcacatcat
     1141 ccggccccga agccagcaga cagacgatct tggtcccgcc cagttcgacg cctgcgatca
     1201 gaggagcgga aaggtcttca gtcattcgat cacccggaat ggcgccaacg cgaaccggct
     1261 tctcgaccga acccgcccca ccttcaaggc ccacgcatta tcagggcttg gcgccgtcgc
     1321 cagagcggga gagggtggca cctcacacaa acgcggacgc ccctcctcat ggccgccaag
     1381 atcaccctgc ccgcccgccc cgagtcgctg gagatcgatc cggcgatcac cgccgtcatc
     1441 gtcatcgaca tgcagaacgc ctacgcctcg ccgggcggct atctggacct cgcgggcttc
     1501 gacatctcgg gcgccgccaa ggtcatcacc aacatcaagg gggtgctcga ggtcgcgcgc
     1561 ggggccggcg ttcaggtgat ctatttccag aatggttggg atcccgacta tgtcgaggcg
     1621 ggcggccctg gctcccccaa cttccacaag tccaatgcgc tgaagaccat gcgcgcccgc
     1681 cccgagctgc acggccagct tctggcgcgc gggacctggg actacgaact ggtcgatgag
     1741 ctgaagcccc agcccggcga catcagcctg cacaagaccc gctattccgg cttcttcaac
     1801 tcccagctcg acagcgtcct gcgctcacgc ggcatccgca acctcgtctt cgtcggcatc
     1861 gccaccaacg tctgcgtcga gtccacgctc cgcgacggct tcttcctcga gtatttcggc
     1921 gtcgtgctcg aggacgccac ccatcaggcc gggccggatt tcgtccagca ggccagcctc
     1981 tacaacatcg agaaattctt cggctgggtc tccagtgtcg ctgacttcaa gggcgtcgtc
     2041 ggccagatcg cgcccgccac ccaacaggag acctaacgtg cccaagaccg tcatcacccc
     2101 gcccggaacc gtcaccccga tcgccccctt ctcgcccggc acgctggcgg acggcgtggt
     2161 ctatgtctcg gggacgctgg ccttcgacaa ggacaacaat gtcgcctgcg tcggtgacgc
     2221 cgagggccag acgcgcatcg tgctggagac catcaagtcg gtgattgaga ccgccggcgg
     2281 gaccatggac gacgtcacca tgaaccacat cttcctcaag gactgggccg actacccggc
     2341 gctgaacaag gtctacgccg agtatttccc gggcgagaaa cccgctcgtt tttgcatcca
     2401 gtgcggcctc gtccgccccg acttcctcgt cgagatcgcc accatcgcgc atatcggcaa
     2461 gacgtgaatc ccctcgccac cctgctgatc aaggccgtcg tctccgcgat cctgatcgtg
     2521 gcggcgtccg aggtggcgcg tcgacagccc ggcgtcggcg ccttgatcgc atccctgccg
     2581 ctggtctcgg tgctcggcat gatctggctg tggtcatcaa agcccgacgc cgagaacatg
     2641 gcccagcacg cccaggcgac cttctggtac gtcctgccgt cgctgccgat gttcctcgtg
     2701 atcccggcgc tgctgcgacg cggcgtcccg ttctggcctg cgctgggcct aggctgcgcc
     2761 ctgactgtag gcctctattt ggcgatg
//
LOCUS       DASVTM010000373         8435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_884851,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000373 DASVTM010000000
VERSION     DASVTM010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884851"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="VF633_10135"
     CDS             <1..1581
                     /locus_tag="VF633_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8471461.1"
                     /translation="NGAEPAFVTAVSISVVVFVAYCISTWRSMNDAYTAEIRARIDAE
                     RRGLEAERAMTSRSAFLAAIAHDLRTPIGAILTGASEMDRTTLDGSARANAALITDAG
                     LMMKSLLDNLLDHSKLDAGRMTVEAGDFNLRSMLAQTLRLWAGPMRAKGLSLRVEGAS
                     AVPAMVRGDAMRLRQVLNNLVSNALKFTDEGSVTLRLRSWQEEPSGHALLIEVQDTGP
                     GMTDAQLARLFTPFDQTADGISARHGGTGLGLAISRDLAGLMGGRLTARSLPGEGACF
                     TLSLMLAPATNPEAVVKVLDAASRSDVAKALKTSEISRAALRPRPTPPTPPASPHPSQ
                     AGVVEPAGEPGAAVEALLASLLAGSSLNDQPAPEPDSLEPAPYMASVEDEMDDEEGRP
                     LRVLVVDDHDINRRAVELILAPLGCDIATAADGLAALRRCEDAAFDVIFMDVRMPELD
                     GRETTRRLRAGCGPNAATPVIAVTADTSPDDIAACLAAGMDYFVSKPLTPPALLGALQ
                     QVLSAGEDAAGAAETVAA"
     gene            complement(1473..1988)
                     /locus_tag="VF633_10140"
     CDS             complement(1473..1988)
                     /locus_tag="VF633_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEX8471462.1"
                     /translation="MTTDVFTAEKRSAVMRRVKGKDTAPELAVRRILRAAGIGYRVGG
                     QGLPGKPDVVMKGRGVALFVHGCFWHGHDCPRGSRQPKTNADYWISKIGRNRVRDAHN
                     DEILQAEGWRVVTVWECSMKAPDFAADLIATIKGQAATVSAAPAASSPAESTCCSAPS
                     SAGGVRGLETK"
     gene            complement(2077..2559)
                     /locus_tag="VF633_10145"
     CDS             complement(2077..2559)
                     /locus_tag="VF633_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8471463.1"
                     /translation="MTIQIGDAIPAATLGTPASDGPRPITTDEIFKGKTVALFAVPGA
                     FTPTCSARHLPGYIDNGADFKAKGVDTIACLSVNDPFVMGAWAKATDGSEDILMLADG
                     SGDFTRELGLTLDARGFGMGERSQRYSMLVKDGIVTELNIESGGEFKVSSAEHLLAQL
                     "
     gene            2724..3293
                     /locus_tag="VF633_10150"
     CDS             2724..3293
                     /locus_tag="VF633_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX8471464.1"
                     /translation="MEPFQSLVGRLLVAMPGIGDPRFEHAVILVCVHEPDHAMGLRLD
                     RPAPMVDLKSVLSKLEIPAPDSAIGRPVMVGGPVDAERGFVLHTDDWMSPDASVPFVT
                     GLAMTGTKDALLAMTDVASGPRRSKLLLGYAGWGEGQLEDELGENVWLTADADLDVIF
                     DGDHETKWSRALAGMGVDAAFLSGQAGRA"
     gene            complement(3397..5919)
                     /locus_tag="VF633_10155"
     CDS             complement(3397..5919)
                     /locus_tag="VF633_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471465.1"
                     /translation="MNRKRAFWASTALFTGMIVAGAASAQSTGSSATEVEEVVVTGSR
                     GPATAGVAIAETVAKTRNTINQEFISTQAAGQTILQTLNLTPGLSFTNADPYGNSGGN
                     IRLRGFDGSRVSLTFDGIPLNDTGNYSSYTNQQLDPELIERASVNTGSTDVDSPTASA
                     TGGTINYQTLRPTADMGGWLQGSLGDFNYRRVMGLFNTGEIGPWGTTAWLAASYTDYD
                     KFKGIGELEKTQYNAKIYQPLGDNGDFIALSGHYNENRNNNNQGPNLATTTGFCGAPG
                     TATATTPCLDQVKTSPFGWDVDFDRTYVAPTVRAGVADIDTNSANYWGLRINPSDTGN
                     VRGNSRFTLMDGLVLTVDPSFQYTLADGGSQQAVLAENSQLLRGTKATGGVDLNGDGD
                     LLDSVRVMTPSVTNTHRYGLNSSLIWDINDTNRLRVAYALDYGRHRQTGQYGKIDFSN
                     PTDPKFVDPFGGRNEEDNRIVNLDGYVLQARDRKSIAELNQFSVEYRGKFLQDALTVS
                     LGVRAPFFKRELNQYCYSQNSSSNVLCTSQLPNAALANGNVTFGAATTQYIPPYAREL
                     KYDDILPNLGATYRFGEGQSIYGSYSESLSAPRTDSLYTVRRNPTTNVIDNPTVQPET
                     SKNYDLGYRFTTSTVVLQASVFANEFDNRIVSTYDPELDTFVDRNVGSVKSTGAELSA
                     GWEPIENLSLYGTASFLDSELQDNYVLNAAGAVAATKGKKQVETPEEMYSARISYKFN
                     EVFAAGIQAKYTGERWVTDVNDQKVDAYTVVDLDARFDFATLGLDGTYLQFNVTNLLD
                     EQYYSTIGTRTTGTPGQPGYSSPAFAGVGAPRTVMATLRYSF"
     gene            complement(6118..6771)
                     /gene="msrA"
                     /locus_tag="VF633_10160"
     CDS             complement(6118..6771)
                     /gene="msrA"
                     /locus_tag="VF633_10160"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8471466.1"
                     /translation="MPIHSRLTTLAAVLAIAACSQGETPAAAAPASTAASQAGGQPFR
                     DSGRREVAVFAGGCFWSVESNMEHIPGVVSAISGYAGGTSPNPTYEAVEAGNGYVEAV
                     QVTFDPARISYRQLVDRFWRTIDPTDARGQFCDTGPAYGTAVFATVAQKPTADASRQA
                     AAAAIGARRFTTRVRNAGRFWPAEAYHQDFARLNPVRYGGYSRFCGRAARLRAIWGA"
     gene            complement(6870..7961)
                     /locus_tag="VF633_10165"
     CDS             complement(6870..7961)
                     /locus_tag="VF633_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEX8471467.1"
                     /translation="MIFNAGDVLYGRGPGTIYFKGSAAQWPERLSMLLPAYTDVIVFG
                     EGGPYNQAVLRNTSGIDAHVWVLENGYFRPDWITVERNGVNGSSALPRDRKAYEPPAP
                     DTPVTRPVGKILPYHVLNISLYHLIQLPGRWMFPRYAAPYTQAPWLQCAGHIRRYFGL
                     AFQPRNRCDTPAIARQGPFFIACLQREGDAQLLRYSHFADNTAFLAEVMNSFAGHAPA
                     DARLVVKNHPLDPGLVNLGRITRLLAEERGIAKRVDFIDGGNLAQLCRASRGMVVNNS
                     SAALSALGFHTPVKALGQAFFDFEGLTDQKPLDLFWSDPAEPDPDLFARFRAHVIARS
                     QVNGNYHEPRSMQPTALALASVFENPSRL"
BASE COUNT         1534 a         2511 c         2815 g         1575 t
ORIGIN      
        1 aatggagccg agccggcctt cgtcaccgcc gtctccattt cggttgttgt gttcgtcgcc
       61 tattgcatca gcacctggcg cagcatgaac gatgcctata ccgccgagat ccgggcccgt
      121 atcgacgctg aacgtcgcgg tctggaggcc gagcgcgcca tgacgagccg gtcggccttc
      181 ctcgccgcca tcgcccatga tttacgtacg cccatcggcg ccatcctgac cggggcgtcc
      241 gagatggatc gcacaacgct ggacgggtcg gcccgcgcca acgccgccct gattaccgat
      301 gcggggctga tgatgaagag cctgctcgat aacttgctgg accattccaa gctggatgct
      361 ggccggatga cagtcgaggc tggcgacttc aatctccgct cgatgttggc gcagacccta
      421 cgtctctggg ccggtccgat gcgcgccaaa ggcttgtccc tgcgggttga gggcgccagc
      481 gccgttccgg ccatggtgcg tggcgacgcg atgcgcctgc gccaggtgct caacaatctg
      541 gtctccaacg cattgaagtt cacggacgag ggttcggtca ccctgaggct acgatcctgg
      601 caggaagagc cttcgggtca cgcccttctg atcgaagtcc aggacacggg cccgggcatg
      661 accgacgccc aactggcgcg cctgttcacg ccgttcgacc agacggccga tggcatcagc
      721 gcacgccatg gcggcacggg cctggggctg gccatcagcc gtgatctggc gggtctgatg
      781 ggagggcgcc tgaccgcacg tagcctgccg ggcgaagggg catgttttac gctgtcgttg
      841 atgctcgctc cagcgaccaa cccggaggcc gtcgtcaagg tccttgacgc agccagtcgc
      901 agtgacgttg ccaaagccct gaagacgtcg gagataagcc gtgccgcact gaggccgcgg
      961 cccacgccgc ccacaccgcc cgcttcgccc cacccatcgc aggccggtgt cgtagaaccg
     1021 gccggggaac cgggcgccgc tgtcgaggcg ctgctggcgt ctctgctggc aggatcaagc
     1081 ttgaacgacc agccggctcc tgaacctgac tctctcgaac ccgctccgta catggcgtca
     1141 gtcgaagacg aaatggacga cgaagagggt cgtccgctga gggtgctggt cgtcgatgac
     1201 catgacatca accgtcgtgc ggtggagctg attctcgccc cgctgggttg cgatatcgcg
     1261 acggccgccg acggtctggc tgccctgcgg cgctgcgagg atgctgcttt cgacgtgatc
     1321 ttcatggatg tccgcatgcc cgaactcgac gggcgcgaga ccacgcgtcg cctgcgtgcc
     1381 gggtgcggcc caaacgctgc gactcccgta atcgcagtca ccgccgacac ttctccggac
     1441 gatattgcag cctgcctggc cgccgggatg gactatttcg tctccaagcc cctgacgccg
     1501 ccagcgctgc tgggtgcact gcagcaggta ctctcggctg gcgaggatgc tgccggagcc
     1561 gccgagaccg tcgccgcctg acctttgatc gtcgcgatca gatcggctgc aaagtccggc
     1621 gccttcatcg aacattccca gactgtcaca acccgccagc cctctgcctg caggatctcg
     1681 tcgttatgcg catcgcggac acggttgcgg ccaatcttgg agatccagta gtcggcgttg
     1741 gtcttgggct gtcgtgaccc gcgtgggcag tcgtggccgt gccaaaagca gccatgcacg
     1801 aacagggcga caccccggcc cttcatcacc acatccggct tgccgggcag gccttggccg
     1861 ccgacccggt agccgatgcc ggccgcgcgc agaatgcgtc gcacggccag ttcgggagcg
     1921 gtgtccttgc ccttcacccg ccgcatgacg gcgctgcgct tctcggcggt aaagacgtcg
     1981 gtggtcatcg tagaaagact gaacaggcag tagcgagagg acgagccaag gagcgctcgc
     2041 cccttggctc aacggcgctc tttaccctgc cttcgatcag agctgcgcca gcagatgttc
     2101 ggccgaggag accttgaact cgccgccgct ttcgatgttc aactcggtca caattccgtc
     2161 cttgaccagc atcgaatagc gctgcgagcg ctcgcccatg ccgaagccac gtgcgtccag
     2221 cgtcagaccc agctcgcggg tgaaatcgcc cgaaccgtcc gccagcatca ggatgtcctc
     2281 ggagccgtcc gtggccttgg cccaggcgcc catgacgaag ggatcgttga cggacaggca
     2341 ggcgatggtg tccacgccct tggccttgaa gtccgcgccg ttgtcgatgt agccgggcag
     2401 gtgacgcgct gagcaggtcg gggtgaaggc gcctggaact gcgaacaggg cgacggtctt
     2461 gcccttgaag atttcgtcgg tggtgatcgg cctggggccg tccgaggcgg gggtgccgag
     2521 ggtcgcggcg ggaatggcgt cgccgatctg gatggtcatg gagatctccg ggggaatgtc
     2581 tggctgaaga agcgctgctt cacacagcag ataggctcag gcggttgcgc cgccaagacg
     2641 caaagcgggc gatccggccc ggccgacgcc ggctttgatc gccagccctt gcaaagggag
     2701 cgtggggcgc cgatcattca ggtatggaac ctttccaatc cctcgtcggg cgcctgcttg
     2761 tggctatgcc tggtatcggc gatccacgct tcgagcacgc ggtgatcctc gtctgcgtcc
     2821 atgagcccga ccacgcgatg gggcttcggc tggaccggcc agcgccgatg gtggacctta
     2881 aatccgtact gtccaaactc gagatcccgg ctcccgacag cgccataggt cgccctgtca
     2941 tggtcggcgg accggtggat gcagagcgcg gcttcgtcct gcacaccgac gactggatgt
     3001 cacctgacgc cagcgtgccg ttcgttaccg gcctggcgat gaccggcacc aaggacgcgc
     3061 ttctggccat gaccgatgtc gcctccggcc cccgccgttc caagctcctg ctcggctatg
     3121 cgggatgggg ggagggccag cttgaggatg aacttggtga gaacgtctgg ctgactgccg
     3181 acgccgatct ggacgtgatc ttcgacggcg accacgaaac gaagtggagc cgcgcgttgg
     3241 ccggcatggg cgtggatgcc gccttcctgt cgggacaggc tggccgcgcg tagggtctag
     3301 ccagccgtat cagcggaatc ttgaatggcg tgagcggaca aagaaaaagg gcggtccgtt
     3361 tccggaccgc ccctgatcgt cttgagtggc tactgtttag aacgagtagc gcagggtcgc
     3421 catgacggta cgcggagcgc cgacgccagc gaatgccggg ctgctgtagc ccggttggcc
     3481 cggcgtaccc gtcgtacggg tgccgatggt cgagtagtac tgctcgtcca gcaggttcgt
     3541 gacgttgaac tgcaggtagg tgccgtccag gccgagggtc gcgaagtcaa agcgggcgtc
     3601 gagatcgacg acggtatagg cgtcgacctt ttggtcgttg acgtcggtga cccagcgttc
     3661 gccggtgtat ttggcctgaa taccggccgc gaacacctcg ttgaacttgt agctgatgcg
     3721 agccgagtac atttcttccg gcgtctcgac ctgcttcttg cccttggtcg cggcgacagc
     3781 gccggcagcg ttcaggacgt agttgtcctg cagttcgctg tcgaggaacg aagccgtacc
     3841 atagaggctc agattctcga tcggttccca gcctgccgac agttcggcgc ctgtggactt
     3901 aaccgagccc acgttccggt cgacaaaggt gtccagttcc gggtcgtagg tcgagacgat
     3961 ccggttgtcg aactcgttgg cgaagaccga agcttgcaga acgacggtcg aggtcgtgaa
     4021 gcgataaccc aggtcgtagt tcttagaggt ttcgggctga acggtcgggt tgtcgatgac
     4081 atttgtcgtc gggttgcggc ggacggtgta gaggctgtcc gtacgcggag ccgaaaggga
     4141 ttccgaatag ctgccgtaga tcgattggcc ctcgccgaag cgataggtcg caccaaggtt
     4201 gggcaggatg tcgtcgtact tcagttcacg ggcgtaaggc ggaatgtact gggtcgtcgc
     4261 ggcgccgaag gtcacgttac cgttcgccag agcggcattc ggaagttgcg aggtgcagag
     4321 cacgttcgac gaagagtttt gcgagtagca gtactggttc agttcacgct tgaagaaggg
     4381 cgcgcgcaca ccgaggctca cggtcagagc gtcctgcagg aactttccgc gatattcgac
     4441 cgagaactgg ttcagctcgg caatagactt gcgatcgcgt gcttgaagga catagccgtc
     4501 aaggttgaca atgcgattgt cttcttcgtt acggccgccg aacgggtcga cgaacttggg
     4561 atcggtcggg ttggagaagt cgatcttgcc gtactggccg gtctggcggt gacgtccgta
     4621 gtccagcgca taggcgacgc gcagacggtt ggtgtcattg atgtcccaga tcagcgagct
     4681 gttcaggccg tagcggtgag tgttggtcac cgatggggtc atgacgcgaa cgctgtcgag
     4741 gagatcgcca tcgccgttca ggtcgacacc gccggtcgct ttggtgccgc gcagcagttg
     4801 gctgttttcc gccagaacgg cttgttgcga gccgccgtca gccagtgtgt actggaacga
     4861 tgggtcgacc gtcaggacca gaccgtccat cagggtgaag cgcgagttac cgcggacgtt
     4921 accagtgtcc gacgggttga tccgcagacc ccagtagttg gcgctgttgg tatctatgtc
     4981 agcaacaccg gcacgaaccg ttggtgcaac ataggtgcgg tcgaagtcga cgtcccagcc
     5041 gaatgggctg gtcttgacct gatcaaggca gggcgtggtc gcagtggcgg tgccaggcgc
     5101 gccgcagaag ccggtcgtgg ttgcgaggtt ggggccctga ttgttgttgt tgcggttctc
     5161 gttgtagtga cccgacaagg cgatgaagtc gccgttgtcg ccgaggggct gatagatctt
     5221 ggcgttgtac tgggtctttt ccagctcgcc gatgcccttg aacttgtcat agtcggtgta
     5281 ggaggcggcg agccatgcag tcgtgcccca agggccgatc tcgccggtgt tgaacaggcc
     5341 catgacgcgg cgatagttga agtcgcccag cgaaccctgg agccagccgc ccatgtcggc
     5401 ggtcggacgc agcgtctgat agttgatggt gccaccagtg gccgaggccg tcgggctgtc
     5461 gacatcggtc gagccggtgt tgaccgaagc gcgctcgatc aattcggggt ccaactgttg
     5521 gttggtgtag ctcgaatagt tgccggtgtc gttcagagga atgccgtcga aggtcagcga
     5581 tacgcgcgaa ccgtcgaagc cgcgcaggcg gatgttgccg cccgagttgc cgtagggatc
     5641 ggcattggtg aagctgaggc ctggtgtcag gttcaacgtc tgcaggatgg tttggccagc
     5701 ggcctgtgtg gagatgaact cctgattgat cgtgttacgg gtcttggcga ccgtctcggc
     5761 gatcgcgacg ccagcggtcg caggaccacg cgagccagtc acaacgactt cttcaacttc
     5821 cgtagcggac gaaccggtcg attgtgccga ggcggcgccg gcgacgatca tgccggtaaa
     5881 gagggccgta gaagcccaga acgcgcgttt gcggttcatc attgtatctg tatccccaga
     5941 tgatgcgggc aggcgcgccc atggccgtca ggtggctgac ctgacgaaat cctgccccat
     6001 ctagggcccc tattgtaatg tcggacgaca gtaatgcgac ggtgatatga cagcgcaaca
     6061 gggtggccgc agtgccacag tcgccgctac ttacctttcc ggcgctccgg ttaggaacta
     6121 cgctccccag atcgccctta gacgggcagc ccggccgcag aatcgcgagt aacccccgta
     6181 gcgaaccgga ttgagcctcg caaaatcctg atggtacgct tccgccggcc agaagcggcc
     6241 ggcattgcga acccgcgtcg tgaagcggcg agcgcctatc gccgctgcgg cggcctgccg
     6301 ggaggcgtcg gcggtgggct tctgggcaac agtcgcaaac acggctgtcc cgtaggcagg
     6361 gccggtatca cagaactggc cgcgagcgtc agtgggatcg atggtgcgcc agaaccggtc
     6421 cacgagctgg cggtaactga tccgggcggg gtcgaaggtc acctgaacgg cttcgacata
     6481 cccgttgccc gcctcgacag cctcgtaggt ggggttcggc gacgtcccgc ctgcatatcc
     6541 agagatggca gagaccacgc ccgggatatg ctccatgttg gactcgaccg accagaaaca
     6601 gccgccggcg aaaaccgcaa cctctctgcg gccactatca cgaaagggct gacctccggc
     6661 ttgcgacgcg gctgtggacg ctggggcggc ggctgcaggc gtttcgcctt gcgaacaggc
     6721 ggcgatggcc aggacggcag cgagggtggt cagacgggag tgtatgggca ttgcgtgtct
     6781 cgatgacgat aggcgatgct cgcccaccac cagatacgtc cgaacccccg aaccggatac
     6841 atcgtcaggt tcagcgatta tgaccgcagt cagagacgcg acgggttctc aaacaccgat
     6901 gccagcgcca gcgctgtcgg ctgcatggag cgcggctcgt gatagttgcc attgacctga
     6961 ctgcgggcga tcacgtgagc ccggaagcgg gcgaacaagt cgggatccgg ctcggccggg
     7021 tctgaccaga atagatcgag tggtttctgg tcggtcagcc cctcgaaatc gaagaaggcc
     7081 tgaccgagcg ccttcaccgg ggtatggaac cccaaagcgg agagagcggc gctcgagttg
     7141 ttgacgacca taccgcgcga agcccggcaa agctgggcca ggttgccgcc gtcgatgaaa
     7201 tccacccgct ttgcgatgcc gcgttcctcg gcgaggaggc gcgtgatacg acccagattc
     7261 accagacccg gatcaagcgg atggttcttg accacgaggc gtgcgtcagc tggcgcatgg
     7321 ccggcaaagc tgttcatcac ctctgccaga aaagcggtgt tgtcggcgaa atgagagtat
     7381 cgcagtagct gggcatcccc ttcacgctga agacaggcaa tgaagaacgg cccctgtcgg
     7441 gcgatggcgg gggtgtcgca gcgatttctc ggctggaacg cgaggccgaa atagcggcgg
     7501 atatgcccgg cgcactgaag ccacggcgcc tgggtatagg gagctgcata gcgcgggaac
     7561 atccaccgtc ctggcaactg gatcaggtgg taaagactga tattcagaac gtggtagggc
     7621 agaatcttac ccacgggtcg tgtcaccgga gtatcgggag ccggaggctc ataggccttg
     7681 cgatcccgag gaagggcgct ggagccgttg acgccgtttc gctcgactgt gatccagtcc
     7741 gggcggaagt agccgttctc caggacccag acatgggcgt cgataccgga ggtattgcgc
     7801 aacaccgcct ggttgtaggg gccgccctca ccaaagacga tgacgtcggt ataggcgggg
     7861 agaagcatac tcaggcgttc gggccattgc gctgcgctgc ccttgaaata aatcgtgccc
     7921 ggtccccggc cgtacaggac gtcgcctgcg ttgaagatca ttcgggagac gtcagctccc
     7981 cgtgatctca aagcttcggc caaaacgcgt ccaaaaggac cgaaaggcgc cgtgatgatc
     8041 agaaaacgcc gtccagccaa gccagagggc cggacgttgg cgagcgtaag agagggctcc
     8101 tcgggatagt caggatcaag attggcgagc aaacccgtgg actttctgag tgctgcgcaa
     8161 ggcgcgccct aatagggcgc catctcctgc tgtgacaagc gggctcgcgt ggaagtcgtc
     8221 caaaagagtg tccattccgt catccccgag gccggaagag tgttggacgc cgttcgaagc
     8281 gaaggcatgc cgcgaaagca aaaccgccgg tcctgctttc gcgggaccgg cggtctgaag
     8341 tcttcgacgt cgttcgagac cgttggggtc cgcagtgatg cggacccctt tggactagaa
     8401 gttgatgtcg atggtggcgc gacggttcag aggct
//
LOCUS       DASVTM010000374         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_940974,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000374 DASVTM010000000
VERSION     DASVTM010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940974"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VF633_10170"
     CDS             complement(<1..966)
                     /locus_tag="VF633_10170"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX8471468.1"
                     /translation="MNLTSKAGGPLNGTVRAPGDKSMSHRSMILGAMASGVTEVEGLL
                     EGDDVLATARAAEAFGAMVEKLDTGKWRITGNGGFQSPDDVIDCGNAGTGVRLLMGAA
                     AGYPITAAFDGDASLRSRPMGRVTDHLTCMGATFDWSGKAGLLPATLTGGALSAIDHV
                     QTVASAQVKSAILLAGLNADGVTSVTEPEKSRDHTERMLRAFGAVVDVEEDGEGWTIR
                     LQGGQPLTGTKVSVPGDPSSAAFPLAAGLIVPGSSVTVEGVMLNPLRTGLFDTWIEMG
                     ADLTITNRRESGGEDVGDITARYSALKGVVVPKARAASMIDEYPIL"
     gene            1144..1605
                     /locus_tag="VF633_10175"
     CDS             1144..1605
                     /locus_tag="VF633_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX8471469.1"
                     /translation="MADPQLGAKQVCPNCQAKFYDLTRRPAHCPKCDTEFDPEEAIKL
                     RSRRGRPGGYPADDAEDQVEEKKVSDDEDEEEEVATPEIDQEGHEPILTPDDEDDAVA
                     DPSEESGMGTTDGDDDVLADDDDDDSVPFIEDEDDDAFDGDLAKGSKDDDL"
     gene            1818..1893
                     /locus_tag="VF633_10180"
     tRNA            1818..1893
                     /locus_tag="VF633_10180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1851..1853,aa:Ala,seq:ggc)
BASE COUNT          421 a          683 c          639 g          342 t
ORIGIN      
        1 gaggatcggg tactcgtcga tcatcgaggc ggcgcgagcc ttggggacca cgacgccctt
       61 caaagcagaa tagcgggcag tgatgtcgcc gacatcctcg ccgccgctct cgcgccggtt
      121 tgtgatggtc aggtccgcgc ccatctcgat ccaggtatcg aaaaggccgg tgcgcagggg
      181 gttgagcatg acgccctcga ccgtcacgga agatcccggc acgatcaatc ccgccgccag
      241 cgggaaggcg gccgaggacg gatcacccgg cacggaaacc ttggttcccg tcaaaggctg
      301 gccgccctgc agacgaatgg tccagccctc gccatcttcc tcgacatcca ccactgcccc
      361 aaaggcgcgc agcatccgtt cggtgtgatc gcggctcttt tccggctcgg tgacggaggt
      421 cacgccgtct gcgttcagcc cggccagcag gatcgccgac ttgacctggg ccgaggcgac
      481 cgtctggaca tgatcgatgg ccgacaaagc gccgccggtc agggttgccg gcagaagacc
      541 ggccttgccg ctccagtcga aggtcgcgcc catgcaggtc agatgatctg taacccggcc
      601 catcgggcgg ctgcgcagcg aggcgtcgcc gtcgaaggcg gctgttatcg gatagcccgc
      661 cgccgcgccc atcagcagcc gaacgccggt gcctgcattg ccgcagtcga tgacgtcatc
      721 gggcgactgg aagccgccgt tgccggtgat gcgccatttg ccggtgtcca gcttttccac
      781 catcgcgcca aacgcctcgg ctgcgcgggc ggtggctagg acgtcgtcgc cctccaacag
      841 cccctcgact tcggtcacgc cgctcgccat cgcaccaaga atcatcgaac ggtgggacat
      901 cgacttgtcg ccaggcgccc gcaccgtgcc gttcaagggg ccgccggcct tgctggtgag
      961 attgatggag gtgcgggtct ggtcggaggc tgaagacacc gattgggctc cggattgacg
     1021 gcaagagagg ggttcgcccc ataaccgcta ccgtcgggac caaagacggc ttttgacagc
     1081 gaggccccac agtggcaagg gacgcgacct aaatccccac atccattcaa aggctctcaa
     1141 cacgtggccg atccccaact tggcgccaag caggtctgcc cgaactgcca ggccaaattc
     1201 tacgacctga cccgtcgccc tgcgcattgc ccgaagtgcg acaccgaatt cgatcctgaa
     1261 gaagcgatca agctgcgcag ccgtcgtggc cgtccgggcg gttaccccgc tgacgacgcc
     1321 gaggatcagg ttgaagagaa gaaggtctcc gacgacgagg acgaagagga agaggtcgca
     1381 acccccgaaa tcgaccagga aggccatgag ccgatcctga cccccgatga cgaggacgac
     1441 gcggtcgccg atccttccga agagtccggc atgggcacca ccgacggcga cgacgacgtt
     1501 ctggcggatg acgatgatga cgactccgtg cccttcatcg aagacgaaga cgacgatgcc
     1561 ttcgatggcg acctcgccaa gggcagcaag gacgacgacc tctaggtcat cgcatatttc
     1621 gtgactcaag atccgggccc tcgcctttcg caaggcgtcg gcccggtctg aaagccgctt
     1681 tcggatcgtt ctgaagcggt ggaaaataaa agcggcagcg gggttgatcg agaaatcgcc
     1741 ctgacatact aacgccgcct cgccggggac taccaaaaac ccgacgtacc gatcgaaagg
     1801 ctcagcgccc tcggttcggg gctttagctc agctggtaga gcgcttgcat ggcatgcaag
     1861 aggtcagcgg ttcgactccg ctaagctcca ccaattccaa ggggttagca ggacactgct
     1921 aacccctttt gcttgctggg caacaccgga gtaacaaaca acgttacgcc gagagtttcc
     1981 ggccccggcg tttctcaccc ttttccagcg cggttcagcc cgtcaggcga cggccaaaaa
     2041 gccgtgagtg ccaacacccg ctgaacagcg gccgtattcg ccaca
//
LOCUS       DASVTM010000375         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_460251,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000375 DASVTM010000000
VERSION     DASVTM010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460251"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VF633_10185"
     CDS             complement(<1..359)
                     /locus_tag="VF633_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX8471470.1"
                     /translation="MALAPAVKKRLPLIVAGVVVIALLIGGFFWWQGKQRWESTDNAF
                     VQADTTLVSPQINGYVTEVLVSDNQRVQPGQVLVKLDDSDARAQLAQAEANLAALIAA
                     VGNVDARATQEQAMIASR"
     gene            complement(512..640)
                     /locus_tag="VF633_10190"
     CDS             complement(512..640)
                     /locus_tag="VF633_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471471.1"
                     /translation="MLILAIAVLFTFGAILAWDLAGQPTRLRHFRKLLGLKSRRKR"
     gene            773..2044
                     /locus_tag="VF633_10195"
     CDS             773..2044
                     /locus_tag="VF633_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX8471472.1"
                     /translation="MTSPSSDIRALPEGQSAKKGPGFPEFVCMIALMMALNALAIDSM
                     LPALPAMGDALGVTSDNTRQWIVTAYLLGFGGAQIIYGPLADRFGRKPILLIGVGIYV
                     AFSLVAMLAPTFETLIIARIGQGLGSAATRVLAVSIVRDRFEGRTMARVMSLSFLVFL
                     GVPILAPSLGQLILLVAPWEGIFAVLAFAGVALMIWTGLRLPETLHPEDRLPIEAKRI
                     ALAFKSVLTNRQAMGYTLAMAAVQGALFGFINSSQQIFFDVFEAPGLFTTVFAVIAGG
                     IAIASIVNARIVVKLGSRKIGHTALMGFTALAAIHAAVVISGHESIWTFAILQALTMF
                     CFGLMAGNFGSMAMEPMGHIAGTASSVQGFISTILGSLTGFAIGQQFNGTVVPMALGF
                     TGCGLVAIAAVLFAERGKLFVSQNAVPVPAG"
     gene            2119..>3569
                     /locus_tag="VF633_10200"
     CDS             2119..>3569
                     /locus_tag="VF633_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase/hydrolase"
                     /protein_id="HEX8471473.1"
                     /translation="MSRKTTVSILALSAALAGVLAVAACEQKAAAPTSDMPPLPASAT
                     APVTYPKDVHSYAQPQIARVKHVDLDLTANFAAHTLSGTAALDITAEPGATQIVLDSR
                     NLDIKAVRDAKGPLQFVLGAADPILGQSLTVTFPAFTPGESRKIFIDYATKPDAAALQ
                     WLTPAQTAGGKLPFLFSQGQAILTRTWVPTQDSPGVRQTYSARITVPQTLTAVMSAEM
                     LTPNGESAAAVNGEAMHSYRFRMTNPIPPYLMAIAVGDLVFASEGGRVRVWTEPSRLE
                     AVKAEFSEMGKMVDAAEALYGPYRWGRYDVLVLPPSFPFGGMENPRLTFATPTVIAGD
                     KSLVSLVAHELAHSWSGNTVTHAVWGDFWLNEGFTTYFQNRIMEAVYSPARAQQERVL
                     GWDSLQEQLKTQPAADTRLHLDLNGRDPDDGMTDIAYEKGAAFLETIEQTVGREQFDA
                     WLKGYFDRNAFRPMTSELFLQDIRANLIKGDQAL"
BASE COUNT          587 a         1186 c         1156 g          640 t
ORIGIN      
        1 cgcgacgcga tcatcgcctg ttcctgggtg gcgcgggcgt caacattgcc gacggcggcg
       61 atcagggcgg cgaggttagc ctcggcctgc gccagttgcg cgcgagcgtc ggagtcatcc
      121 agtttgacca gcacctgacc cggctgaacg cgctggttgt cgctgaccag cacctccgtg
      181 acgtagccgt tgatctgggg gctgacgagc gtggtgtcgg cctgaacgaa ggcgttgtcg
      241 gtcgattccc agcgctgctt gccctgccac cagaagaagc cgccgatcag cagcgcaatg
      301 acgacgacac cggccacgat caggggcagg cgtttcttga cggcgggagc gagggccatg
      361 ggataccaga cgttggggcg ataaccccgg ggaggaagga cgttaaatag acccaatcgt
      421 ccagaaaaca acctcacagt ctgtaagcga ttctaacagc gcggcggctg tgttcgtttg
      481 gcttgggaca gggtggtcgg cagcactgag gctatcgttt gcgccgcgac ttcaagccca
      541 gcagcttgcg gaaatggcgg agccttgtgg gctgcccggc aaggtcccaa gccaggatcg
      601 cgccgaacgt gaacagcacg gcgatcgcga gaatgagcat ggcgctgtcc tccgtcggaa
      661 ctgtcggcac cttggcaaga cagtgcatgg gacaacagcg taaatgttgc ggcgcagcat
      721 tttgtgatcg gcaacggttg cgatccgtgt cccgggcgcg catttgaccg acatgacgtc
      781 accttcctcc gacatccggg ccctgccaga gggtcaatcc gccaagaagg gcccgggctt
      841 ccccgagttc gtctgcatga tcgccctgat gatggcgctg aatgcactgg cgatcgactc
      901 catgctgccg gccctgcccg ccatgggcga cgcgcttggc gtgacgtcgg acaacacgcg
      961 gcagtggatc gtcacagcct atctgctggg ctttggcgga gctcagatca tctatgggcc
     1021 gctggctgac cggttcggac gaaagccgat cctgctgatc ggggtgggca tctatgtcgc
     1081 cttcagcctt gtggcgatgc tggcgccaac gtttgaaacc ctgatcatcg cccgcatcgg
     1141 tcagggactg ggctcggcgg cgacgcgggt gctggccgtc tccatcgtgc gcgaccggtt
     1201 cgaagggcgg accatggcgc gggtgatgtc gctgagcttc ctcgtcttcc tcggcgtgcc
     1261 gatccttgca ccgtcgctgg gtcagctgat cctgctggtc gcaccgtggg agggcatctt
     1321 cgcggtgctg gccttcgctg gcgtcgccct gatgatctgg actggtctgc gtctgcctga
     1381 gacccttcat cccgaggatc gactgcccat cgaggccaag cggatcgcgc tggccttcaa
     1441 gtccgtgctc accaaccggc aggccatggg ctacaccctg gcgatggcgg cggtgcaggg
     1501 cgcgctgttc ggcttcatca actcgtcgca acagatattc ttcgacgtgt tcgaggctcc
     1561 gggcctgttc accacagtct tcgccgtgat cgcaggcggc atcgccatcg cctcgatcgt
     1621 caacgcccgg atcgtggtca agctgggctc gcgcaagatc ggccatacgg ccctgatggg
     1681 cttcacggct ctcgccgcga tccatgccgc tgtggtcatc agcggtcatg agtcgatctg
     1741 gaccttcgcc atcctgcagg cgctgacgat gttctgcttc ggcttgatgg ccggcaactt
     1801 cggctcgatg gccatggagc cgatgggcca tatcgcgggc acggcctcgt cggttcaggg
     1861 cttcatctcg actatcctcg gctccctgac gggcttcgcc atcggccagc agttcaacgg
     1921 cacggtcgtt ccgatggcgc tgggcttcac cggctgtggc ctcgtcgcca tcgctgcggt
     1981 gctgttcgcc gaacgtggca agctgttcgt gtcgcagaac gcggtccccg ttccagccgg
     2041 gtaggtcccg ctcgataagg ccttgatctc gacgcccttc ggggttcagg tccgctccga
     2101 agtgcaacgg agcgttccat gtcccgcaag actacagtct cgatcctggc cctgagcgcc
     2161 gcactggccg gggtgctggc cgtcgccgcc tgcgagcaga aggctgcggc tcccacctcg
     2221 gacatgccgc ccctgcccgc ctcggcgacc gcgccggtga cctatcccaa ggacgtccac
     2281 tcctacgccc agccgcagat cgcgcgggtc aagcacgtcg accttgatct gacggcgaac
     2341 ttcgccgccc acaccctgtc gggtaccgcg gcgctggaca tcacggccga gccaggcgcg
     2401 acgcagatcg tgctcgacag ccgcaacctc gacatcaagg cggtgcgtga cgccaagggg
     2461 ccgctgcagt tcgtgctcgg cgccgccgac ccgatcctcg gccagtccct gaccgtgacc
     2521 ttcccggcct tcacccccgg cgagagccgc aagatcttca tcgactacgc caccaagccc
     2581 gacgcagcag cgctgcaatg gctgaccccg gcgcagacgg cgggcggcaa gctgcctttc
     2641 ctgttcagcc agggccaggc gatcctgacg cggacgtggg tgccgacgca ggacagcccc
     2701 ggcgtccgcc agacctactc ggccaggatc accgtgcccc agaccctgac ggcggtgatg
     2761 agcgccgaga tgctgacgcc gaacggcgag agcgccgctg cggtcaacgg cgaggcgatg
     2821 cacagctatc gcttccgcat gaccaacccg atcccgccct atctaatggc catcgcggtc
     2881 ggcgatctgg tgttcgcctc cgagggcggt cgcgtccgag tctggaccga accgagccgt
     2941 ctggaggcgg tcaaggccga gttctccgag atgggcaaga tggtcgatgc ggccgaggcg
     3001 ctctacggcc cgtatcgctg gggccgctat gatgtgctgg tcctgccgcc ttcgttcccg
     3061 ttcggcggga tggagaaccc gcgcctgacc ttcgccacgc cgacggtcat cgccggagac
     3121 aagtcgctgg tctcgctggt ggcgcacgag ctggcgcaca gctggtccgg caacacggtg
     3181 acccacgccg tctggggcga cttctggctc aacgagggct tcaccaccta cttccagaac
     3241 cggatcatgg aggcggtcta tagccccgcc cgcgcccagc aggagcgcgt tctcggctgg
     3301 gactcgctgc aggaacagtt gaagacccag ccggcggcgg acacccgact gcatctcgac
     3361 ctcaacgggc gcgatcccga cgacggcatg accgacatcg cctacgagaa gggcgcggcc
     3421 ttcctggaga ccatcgagca gacggtagga cgtgaacagt tcgacgcctg gctcaagggc
     3481 tatttcgacc gcaacgcctt ccggccgatg acgagcgagc tgttcttgca ggacattcgc
     3541 gccaacctga tcaagggcga tcaggcgct
//
LOCUS       DASVTM010000376        15321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_460379,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000376 DASVTM010000000
VERSION     DASVTM010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 15321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15321
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460379"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VF633_10205"
     CDS             complement(<1..645)
                     /locus_tag="VF633_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8471474.1"
                     /translation="MSSPKLKVVLTRRLPDAVETRMRELFDAELNLRDRPMDRAALEA
                     AVQRADVLVPTITDEIDADLINGAGDQLKMIANFGAGVDHIDIDAAVARGIIVTNTPG
                     VLTEDTADLAMSLILAVSRRIVEGAQVVAEGKFEGWTPTWMCGRKLWGKRLGIIGMGR
                     IGQALARRAKAFGMQVHYHNRKPVSPRIEEELGATWWDDLDEMISRMDVISLNCP"
     gene            713..1330
                     /locus_tag="VF633_10210"
     CDS             713..1330
                     /locus_tag="VF633_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX8471475.1"
                     /translation="MSSKAQSGFQSLKTHFLRRALPVAGTIALALGLGGSLGATSEPT
                     ARMPDGRPTPTLQPVPRWLTLKASEVRARSGPGLDYHILWEYHAAGLPVQVIAETLEW
                     RKICDPEGSVVWVHRSVVRSRRSAYNATAQEIPIRSGRSDTALVRARLSPRSIVSLDD
                     CEDGWCHVGGRKVHGWVPQNAVFGGATSALCDARRPAGVRPQSRG"
     gene            1409..1945
                     /gene="fabA"
                     /locus_tag="VF633_10215"
     CDS             1409..1945
                     /gene="fabA"
                     /locus_tag="VF633_10215"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEX8471476.1"
                     /translation="MSQYPSSFDHAALLASGRGELFGPGNAQLPAPPMLMFDRITTIS
                     EDGGEHGKGYMEAELDIHPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWIGGAG
                     RGRALGVGEVKFKGQVEPHVKRVTYKITLKRVINRKLVMGIADGVLEADGVPIYTATD
                     MRVGLFGATAGASEPVAG"
     gene            2033..3262
                     /gene="fabB"
                     /locus_tag="VF633_10220"
     CDS             2033..3262
                     /gene="fabB"
                     /locus_tag="VF633_10220"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEX8471477.1"
                     /translation="MRRVVVTGLGIVSSIGDGAAEVTESLRNAKSGVVAAPDHIKYGF
                     RSQVWAPPSLGVTAEDWTPRVDRRAARFLANGTAWAHIAFEEALKDSGMTPEEIRDDR
                     IGVIVGEGGPSTQIILQAAQTTMEKMAPKRIGPFAVPKAMASGPSAVLSTWFQMRGIN
                     YSISSACATSAHCIGAAAEQIQLGKQDVVFAGGCEDIDWSMSNMFDAMGAMSSDFNDN
                     PSVASRAYDVARDGFVIAGGAGIVVVEEYERAIARGAKIYAEIVGYGANSDGYDMVAP
                     SGEGAQRCMKIAMDQAGNRKIDYLNPHGTSTPVGDSKEMEAVRNVFGADMPLISSTKS
                     LTGHSLGAAGAQEAIYCLLMMQSGFAAESAHIENLDPAFEGMPILRARKDVELTTVMS
                     NSFGFGGTNGTLILAKV"
     gene            complement(3259..4854)
                     /locus_tag="VF633_10225"
     CDS             complement(3259..4854)
                     /locus_tag="VF633_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8471478.1"
                     /translation="MARIGDYAVAAEPVSPDTSGSAVYERFRAEPHTLVIAVVDADRR
                     PVGLVERNAFLSTMAAAFGRELYGRRPIAALMNVAPMIVDAERGAEAFFDTLESSDPG
                     VLLSGFIAVTQDTYVGVGTVLQVLQAGSALYRRRAEEMGELAKGLAIAEAAAQASSRA
                     KSEFLAVMSHEIRTPLNGVLGIAGLMERKLTQEELRPHVQTILSSGQSLLRLLTDALD
                     MSRAEAGMMSLEPAPLSLTVLADDLEALWHARAEEKGLDLRVRTDSNAWVSADVQRLK
                     QLLNNLIGNAIKFTHAGEVSIELGSEVTAEGVRITGTIDDSGPGIADAKAATVFEPFN
                     TGDANREGAGAGMGLAICRQIVERMNGEISVGASPQGGARFRFSIIVASAEAVEAAVA
                     EADPTSHETLHVLVVDDNATNRFLACKLLELFGCTFETAENGRQGVDAAMAGVFDLIL
                     MDIKMPVMDGVAATRAIRALPGSVSAVPIIALTANADERDAIAYRTAGMTEVVQKPIQ
                     PDALLNAIRLALSESEIETAEAA"
     gene            5074..5868
                     /locus_tag="VF633_10230"
     CDS             5074..5868
                     /locus_tag="VF633_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX8471479.1"
                     /translation="MPTGTLMKGKKGLIMGVANANSIAWGIASQLAAQGAELAFTYMG
                     EGLERRVRPLAESVGAKLLIQADVTDDASMDRAFEELEKEFGTIDFVVHSVAFANKDE
                     LKGSFIENTSRDSFLLAMNISCFSFVDVAKRASKLMPNGGAMITMTYLGSERAIPNYN
                     TMGVAKAALEAATRYIARDLGPRGIRVNAISAGAMRTLSLAGISGGRGMIAQGRAMSA
                     MKEDTSMEGVAGAALWLCSDLGFSTTGEVVHVDAGFHMMGLSGDEE"
     gene            5945..7123
                     /gene="rlmN"
                     /locus_tag="VF633_10235"
     CDS             5945..7123
                     /gene="rlmN"
                     /locus_tag="VF633_10235"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX8471480.1"
                     /translation="MSITLDISRVSASPKALASKAPVNLAGLTRAGLRDALVEGGVCP
                     PEKAKMRASQVWRWIHHHGVTDFAAMSNIDKETRSRLEAAFTLARPEIVERQVSKDGT
                     RKYLIRTAPGIEVEAVYIPDVGRAGALCVSSQVGCTLNCTFCHTGTQKLVRNLTAAEI
                     VAQVQVCRDDLEEWPSPSEDRRLSNIVFMGMGEPLYNLDAVADAIDIISDPEGISLSR
                     RRMTVSTSGVVPQLGPLGDRTQAMLAISLHATNDTLRDVLVPLNKKYNLEQLMAGIRA
                     YPGLSNARRVTFEYVMLKGVNDSPAEARELVKLIDGIPAKVNLIPFNPWPGTDYECSD
                     WKTIEVFAAILNKAGFASPIRTPRGRDILAACGQLKSESEKLRASAIRKAEQATVEAA
                     "
     gene            complement(7148..7426)
                     /locus_tag="VF633_10240"
     CDS             complement(7148..7426)
                     /locus_tag="VF633_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471481.1"
                     /translation="MASSTDTVVIHVRLLGEGVDVWRPVQAERLGETTYRIGDELVPE
                     DEAWSFQPGDIVVVEHREGEDEEGRDLVAVARVKDFDEPSWASRRMAG"
     gene            complement(7410..7634)
                     /locus_tag="VF633_10245"
     CDS             complement(7410..7634)
                     /locus_tag="VF633_10245"
                     /inference="COORDINATES: protein motif:HMM:NF023528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5-related ribonuclease"
                     /protein_id="HEX8471482.1"
                     /translation="MVKSPARLSRQMAARGWTSEQIDEARDHGESRATVNSETGRPAT
                     RYIHPVTGRSVVIDDVSGDVIHVGGDGFIY"
     gene            complement(7648..9048)
                     /gene="argJ"
                     /locus_tag="VF633_10250"
     CDS             complement(7648..9048)
                     /gene="argJ"
                     /locus_tag="VF633_10250"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX8471483.1"
                     /translation="MNKPPIPSGTAARTSTGQKIEHAIEKALDPFASALKRATTPTPA
                     PAAPATPGKPGLAISPLAVPFPHIPPIGGVEIATARAGFYKHERDDLVVFRFSRGTTC
                     AGVFTKHKVGSAPVDWCKRHLDSDEGGKDVRALVVNAGCANAFTGKAGADAARRTASE
                     VAKRFGCRQRDVMLASTGVIGVVLDDHKIAAQLPAIESRLDSDAWALAARGIMTTDTF
                     PKGSYAECEIEGQKVRIAGIAKGSGMIAPDMATMLAFIVTDADIHPSVLRALLGLHVR
                     TTFNSVTVDGDTSTNDTCLMFATGTSTAPRIGRVGDRRLKDFSAALDRVMLDLAHQLV
                     RDGEGATKFVKVTVDGATSPASARKLAKSIADSPLVKTAIAGQDANWGRVVMAVGKTD
                     EPVDRDKLAIRFGPNIAAIDGAPSPTYDEAAMTAYMKNPEIDITVDVGSGRASATVWT
                     CDLTKGYIEINGDYRS"
     gene            complement(9045..9974)
                     /locus_tag="VF633_10255"
     CDS             complement(9045..9974)
                     /locus_tag="VF633_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8471484.1"
                     /translation="MIAFAALLAVAACGRGSDADRPPEPGDRAVAKVQDQTIWASDVK
                     REAVAQGLVGEGEPLDVTSDLFRRVLDEVVDQKLLAREAERRGLDNSALAQRRLKATQ
                     ERILGDMLVENVVNGRINDQAVQTLYQEQLRLAKTSEEIRVRLILSRTKPEADAVIGI
                     LAQGATFSAVAQERSIDEATRFSGGDLGYSTVDVMPQAYANALREAPAGSTVGPFQTE
                     GGWAVLRVEDRRKESPPTLEQARPQIVRYLTYEGVRQLLEQLRGKAKVDILLANEPAT
                     GGEQEPASAPPAAAPSTSPPTAALTPATGAPKS"
     gene            10265..13120
                     /gene="secA"
                     /locus_tag="VF633_10260"
     CDS             10265..13120
                     /gene="secA"
                     /locus_tag="VF633_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX8471485.1"
                     /translation="MLGFAKKFFGSSNDRKVKDFLVRVEKINALEPKFAALSDDELRM
                     MTQTFRDRLAGGETADKILNEAFAVAREASKRVLGQRQYDVQLAGGMILNEGGIAEMR
                     TGEGKTLVAIAPAYLNALGGKGVHVITVNDYLARRDAEWMGRVYRFLGMEVGVIVNGL
                     SSGQRQAAYAADITYGTNNEFGFDYLRDNLVYDRNEMVQRPHNFAIVDEVDSILIDEA
                     RTPLIISGPTEDRSDLYKILDGVVKDLIQDKTTFDLDEKQKQALLTEEGSEKIEQILE
                     DGGHFAEDTTGLYDAANISLVHHINQALRANTLYARDKDYIIKGGEIVLIDEFTGRMM
                     TGRRLSEGLHQAIEAKEGVQIMPENQTLASVTIQNYFRLYEKLSGMTGTAATEAQEFG
                     DIYKMDVLEVPTNRPVQRIDYDDEVYRTEREKNLAIAKQIAECHVRGQPILVGTVSIE
                     KSELLSAMLDTYEYKTEVSRTLKPEFAGKPKEAEKLGDAAYDIVWKSEFTGIPHAVLN
                     ARQHEQEAFIVADAGLPGAVTIATNMAGRGTDIQLGGNLEMRLEKWRLDQRNTGVMVS
                     PEDEKAEEVRLKADIAVHKATALAAGGLFVLGTERHESRRIDNQLRGRTGRQGDPGTS
                     KFFLCCEDDLLRIFAGDRLDSIMRSFGVAEGEAISHPWLNRAVETAQKRVETRNYDIR
                     KNLLKYDDVVNDQRKAVFEQRQEFMDATDLSELVGEFRQDVIKDLVDRYMPPKAYAEQ
                     WDIDGLDEKVRSTLGLELPLHEWAAEEGVSNEEVEERLQAAAAARATERLELVGEGQT
                     RGIEKQFLMQQIDMSWREHLVHLDHLRGVIGLRGYGQRDPLNEYKTEAFSLFENLLQE
                     LRHNVTRWLMTVEFRFEAPPSMEMPEFREIHLNPGTGENEMGNPYAQNPEQQFQGDDR
                     SRLPVELLPVGWERTSRNADCPCGSGRKFKHCHGSLI"
     gene            13377..14981
                     /locus_tag="VF633_10265"
     CDS             13377..14981
                     /locus_tag="VF633_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEX8471486.1"
                     /translation="MTNPIDRRDLLVGAAASLSFTASPAFASPVSDTGVVARTTAGRI
                     SGFRDGDIAVFKGVPYGADTARTRFAAPKPPKPWRAVRPCTEFGAPAPQGGGNPGAGY
                     LPDSPWTQDQTSEDCLNLHVWTPSVDKAKRPVLVWFHGGGFSGWTANSPLYDGANLAK
                     WGDVVVVTVNHRLNAFGYLYLAELGGEAFADSGNAGMLDLVLALRWIRDNIAELGGDP
                     DRVTLFGQSGGGAKASVLMAMPAARGLFHRVMTMSGQQVTVVPPEMATTATRKFLTNA
                     GVETPEGLLRLSKDQLIAAGRGVSTAPVLDGRSLIRDPFEPDATPLSRDVPLIMGNTH
                     DETRFLIGLDDPALFDLTWEQLLPALKRSIPNFFGPLSPEATLVWYRDKHPSYSPSQI
                     FFAMTTELRSWRAQVIQADRRAAQPGANTWVYQFDWQSPVMGGNLGAPHCGDIPFFFR
                     NHRETPTMTGGGPETDVVADTMSSALVNFARTGNPNGRGVPEWPEYELPGRVTMQWNT
                     VSKATPDPRGEERRLLGLIPYRQPGT"
     gene            complement(14986..>15321)
                     /locus_tag="VF633_10270"
     CDS             complement(14986..>15321)
                     /locus_tag="VF633_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8471487.1"
                     /translation="DDFGTGFSSLSYLARLPFDTLKIDRYFVLTMDKDEGSAKIVKSV
                     VNLGRDLNLEVVAEGVENAGLAKLLLDASCHYGQGFGYAPALPAQEAEVYLNESLSDG
                     SAPLKQRSA"
BASE COUNT         2699 a         5158 c         4871 g         2593 t
ORIGIN      
        1 cgggcagttc agcgagatca cgtccatgcg cgagatcatc tcgtccagat cgtcccacca
       61 tgtcgcgccc agttcttcct cgatgcgggg gctgacgggt ttgcgattat gatagtggac
      121 ctgcatgccg aaggccttgg cgcggcgagc gagcgcctga ccgatccggc ccataccgat
      181 gatgccgagc cgctttcccc acagcttgcg accgcacatc caggtcggcg tccagccctc
      241 gaacttgccc tccgccacca cctgcgcgcc ctcgacgatg cggcggctga cggcaaggat
      301 caggctcatg gccagatcag cggtgtcctc ggtcaggaca cccggcgtgt tggttacgat
      361 gatgccccgc gccaccgccg cgtcgatgtc gatatgatcc acgcctgcgc cgaagttggc
      421 gatcatcttc agctggtcgc cggccccgtt aatcaggtct gcgtcgattt catcggtgat
      481 ggtcggcacg aggacgtcgg ccctctggac tgcagcttcg agcgcggccc gatccattgg
      541 gcggtccctg aggttcagtt cggcgtcgaa aagctcgcgc atccgcgtct cgaccgcgtc
      601 cggcaggcgt ctggttaata cgaccttaag cttgggggag gacatgggag ccttgaggtt
      661 tcaacaggcg gccgacctca cggttcggcg ccgatgcgac ggatcgacga tagtgtctag
      721 caaagctcag tccgggttcc aaagcctcaa gacccatttc ctgcgccgag ccctcccggt
      781 cgcgggaacc atagcgttgg cgctcggtct tggcggctct ctgggtgcga cgtccgagcc
      841 gaccgcccgg atgcccgacg gccgcccaac gccgaccctg caacccgtgc cccgttggct
      901 gacgctcaag gcgtccgagg ttcgcgcccg ctcaggaccg ggcctcgact accatattct
      961 gtgggaatac cacgccgccg gactgccggt tcaggtcatc gccgagacgc tggaatggcg
     1021 caagatttgc gatcccgaag gctcggtcgt ctgggtccac cgcagcgtcg tgcgctcgcg
     1081 ccgcagcgcc tacaacgcca cggcgcagga aatcccgatc cgctcggggc gatcggatac
     1141 ggccttggtc cgcgcccggt tgtcgccgcg ctcgatcgtc tcgctcgacg actgcgaaga
     1201 cggctggtgc catgtgggcg gtcgcaaggt ccacggctgg gtgcctcaga acgcggtctt
     1261 cggcggcgcg accagcgcgc tgtgcgacgc ccggcgtccg gcgggcgttc gcccccagtc
     1321 cagaggctga agcccgccct caacaggacg gttgagccta tcctcatgct cgtgtaaccc
     1381 cggcgccaca tattcggagt tccatccctt gagccagtat ccgtcgtcct tcgatcatgc
     1441 cgccctgctg gcctcggggc gtggcgagct gttcggaccc ggcaacgccc agctcccggc
     1501 tccaccgatg ctgatgttcg accgcatcac caccatcagc gaggacggcg gcgagcacgg
     1561 caagggctat atggaggccg aactcgacat ccatcccgat ctctggttct tcggctgcca
     1621 cttcatcggg gacccggtca tgcccggctg cctcggcctc gacgccatgt ggcagctggt
     1681 cggcttctat ctgggctgga tcggtggagc cggccggggc cgagcgcttg gcgtcggcga
     1741 ggtcaagttc aagggccagg tcgagccgca cgtgaagcgc gtcacctaca agatcaccct
     1801 gaagcgcgtc atcaaccgca aactggtcat ggggatcgcc gacggggttc tcgaagccga
     1861 cggcgtcccc atctataccg ccacggacat gcgcgtcggc ctgttcggtg cgacagccgg
     1921 cgcatccgaa ccagttgcgg gctaagctac aagtcggttt acgaacataa ccgcgacaaa
     1981 cgcggggcct actcccctcc ggagcgggtg aacggacaag aggaaaatca gcatgcggcg
     2041 tgttgtcgtt acagggctgg gcatcgtctc ctccatcggc gacggcgccg ctgaggtcac
     2101 ggaatcgctg cgcaacgcca agtctggcgt cgtcgccgcc cccgatcaca tcaaatacgg
     2161 cttccgctcg caggtctggg cgccgccttc gctgggcgtg acggccgagg actggacccc
     2221 gcgcgtcgat cgccgcgccg cccgcttcct ggccaacggc accgcctggg cccacatcgc
     2281 ctttgaggaa gccctcaagg attccggcat gacgccggag gaaatccgcg acgaccgcat
     2341 cggcgtgatc gtcggcgagg gcggtccctc gacccagatc atccttcagg ccgcccagac
     2401 gaccatggag aagatggcgc ccaagcggat cggtcccttc gccgtgccca aggcaatggc
     2461 ctccggcccc tcggctgtgc tgtcgacctg gttccagatg cgcggcatca actattccat
     2521 ctcctcggcc tgcgcgacct cggcccactg catcggtgcg gctgccgaac agatccagct
     2581 gggcaagcag gacgtggtct tcgccggcgg ctgcgaggac atcgactggt cgatgtccaa
     2641 catgttcgac gcgatgggcg ccatgtcctc ggacttcaac gacaacccct cggtcgcctc
     2701 gcgcgcctat gacgtcgccc gcgacggctt cgtcatcgcc ggcggcgcag gcatcgtggt
     2761 ggtcgaggaa tacgaacgcg cgatcgcccg tggcgcgaaa atctatgccg agatcgtcgg
     2821 ctacggcgcc aactccgacg gctacgacat ggtcgccccg tccggcgagg gcgcacagcg
     2881 ctgcatgaag atcgccatgg atcaggccgg taatcgcaag atcgactacc tgaacccgca
     2941 cggcacctcg acgccggtcg gcgactccaa ggaaatggaa gccgtccgca acgtcttcgg
     3001 cgccgacatg ccgctgatct cctcgaccaa gtcactgacg ggccactcgc tcggcgccgc
     3061 cggggctcag gaagcgatct actgcctgct gatgatgcag agcggtttcg ccgccgaaag
     3121 cgcccatatc gagaacctgg atccagcgtt cgagggcatg ccgatcctgc gcgcccgcaa
     3181 ggacgttgaa ctgaccaccg tcatgtccaa cagcttcggc ttcggcggca ccaacggcac
     3241 gctgattctg gcgaaggtct aggccgcttc ggcggtctcg atctcgctct ccgataacgc
     3301 cagccggatc gcgttcagca acgcatccgg ctggatcggc ttttgcacca cctcggtcat
     3361 acccgccgta cgataggcta tggcgtcgcg ttcatccgcg ttcgcggtca gggcgatgat
     3421 cggtacggct gaaaccgaac cgggcagggc gcggatcgca cgcgtggcgg cgaccccgtc
     3481 cataaccggc atcttgatgt ccatcaggat caggtcgaac acgccggcca tcgcggcgtc
     3541 tacgccctga cggccgttct ctgcagtttc gaaggtacat ccgaacagtt caagcagctt
     3601 gcaggccagg aagcggttgg tggcgttgtc gtccacgacg aggacgtgaa gagtctcatg
     3661 cgacgtggga tcagcctcgg cgacggcggc ttcgacggct tcggccgaag cgacgatgat
     3721 ggaaaagcgg aagcgggccc cgccctgcgg cgaggcgccg accgatatct cgccgttcat
     3781 ccgctcgacg atctggcgac agatcgccag tcccatcccc gccccggcgc cctcgcggtt
     3841 ggcgtcgccg gtgttgaacg gttcgaacac ggtggcggcc ttggcgtcag cgatgcctgg
     3901 cccgctatcg tcgatggtcc cggtaatgcg aactccctct gccgtaactt ccgacccgag
     3961 ttcgatcgag acttcgcctg catgcgtgaa cttgatggcg ttaccgatca ggttgttgag
     4021 caactgcttc agcctctgga cgtcagccga gacccaggcg ttggagtccg tgcgaacccg
     4081 caagtccaac cccttttcct cagcccgagc gtgccacagc gcttccagat catcggccag
     4141 aacggtcagg ctgagaggag ccggctccag agacatcatc ccggcctcgg cgcgcgacat
     4201 gtccagcgcg tcggtcaaca gccgcagcag gctctgacca gacgacagta tggtctggac
     4261 gtgaggcctc agttcctcct gcgtcagctt gcgctccatc agccccgcga tcccgaggac
     4321 accgttcagc ggcgtacgga tctcatggct cataaccgcc aggaactccg acttggcccg
     4381 gctggacgcc tgcgccgccg cctcggctat agccagaccc ttcgccagtt cgcccatctc
     4441 ctcggcgcga cgacgataca gggcgctgcc cgcctgaagc acctgcagca cggtgcccac
     4501 gccgacatag gtatcctgcg tgacagcgat gaagccgctc agcagcacgc ccggatccga
     4561 cgattccagc gtgtcgaaaa aggcttctgc gccccgctcg gcgtcgacga tcatcggcgc
     4621 aacgttcatc agggccgcga tcggccggcg cccgtagagt tcgcgaccaa aggcggcggc
     4681 catggtcgaa aggaacgcgt tgcgttcgac cagtcccacc ggacgacggt cggcgtccac
     4741 aactgcgatc accagcgtat gaggctcggc ccgaaaccgt tcatagacgg ccgagccgga
     4801 cgtatcgggg ctcaccggct cagccgccac cgcatagtcg ccgatccgcg ccattcgaca
     4861 ctccacaccc tactgaacgc ggccgatata ggggcgggcg cttaacgacc agttcgccca
     4921 acgtgaagct tattcagcaa tttcaatact attcgtcccg gttcggacga ttaaagccaa
     4981 gcttcggaca gccttccccc ccggcgtgcg ccctgctaac cctgcgtcaa atcaatgcgg
     5041 agattatcca tggcgagcgc cgaaggctgg aacatgccca ccgggaccct gatgaagggc
     5101 aagaagggtc tgatcatggg ggtggcgaac gccaactcca tcgcctgggg catagcgagc
     5161 cagctggccg ctcagggcgc cgaactggcc ttcacctata tgggcgaggg ccttgagcgc
     5221 cgcgtccgcc cgctggccga aagcgtcggc gccaaactgc tgatccaggc cgatgtcacc
     5281 gacgacgcct cgatggacag ggccttcgaa gagctggaaa aagagttcgg cacgatcgac
     5341 ttcgtcgtcc actcggtcgc cttcgccaac aaggacgagc tcaaggggtc gttcatcgag
     5401 aacacctcgc gcgacagctt cctgctggcg atgaacatct cctgcttcag cttcgtggac
     5461 gtggccaagc gcgcgtcgaa gctgatgccg aacggcgggg ccatgatcac catgacctac
     5521 ctcggctcgg aacgcgccat cccgaactac aacaccatgg gcgtggccaa ggccgcgctc
     5581 gaggccgcga cccgctatat cgcccgtgac ctcggcccac gcggcatccg cgtcaacgcc
     5641 atctcggctg gggccatgcg caccctgtcc ttggccggta tctcaggcgg tcgcggcatg
     5701 atcgctcaag gccgcgccat gtcggcgatg aaggaagaca cctcaatgga aggcgtcgcc
     5761 ggcgccgccc tgtggctgtg ctcggacctc ggcttctcga ccaccggcga agtcgtccac
     5821 gtcgacgccg gcttccacat gatgggtctg tccggcgacg aggagtaagg gctggagcag
     5881 cacgccttaa atccagcgag atctgtgata taaggcgggc tccctccgat caggatatcc
     5941 gctcttgagc atcacgctcg acatctctcg cgtctccgcg tcccccaagg ctctggcgtc
     6001 caaagccccc gtcaacctcg ccggtttgac gcgcgcgggc ctgcgcgacg cgctggtcga
     6061 ggggggcgtc tgcccgcctg agaaggccaa gatgcgggcc tcgcaggtct ggcgctggat
     6121 ccatcaccac ggcgtcaccg acttcgcggc gatgtccaat atcgacaagg aaacgcggtc
     6181 ccgcctcgaa gccgcattca ccctcgcccg ccccgagatc gtcgagcgtc aggtgtcgaa
     6241 ggacggcacc cgcaaatacc tgatccgcac cgcgcccggc atcgaggtcg aggccgtcta
     6301 catccccgac gtcggccgcg cgggcgccct gtgcgtctcg tcacaggtcg gctgcaccct
     6361 caactgcacc ttctgccaca ccggcaccca gaagctggtc cgcaacctga ccgccgccga
     6421 gatcgtcgct caggttcagg tctgccgtga cgatctggag gaatggcctt cgcccagcga
     6481 ggaccgacgc ctgtccaaca tcgtcttcat gggcatgggc gagccgctct acaatctgga
     6541 cgccgtagcc gacgccatcg acatcatctc cgatcccgag ggcatctccc tgtcgcgccg
     6601 ccggatgacg gtatcgacct cgggcgtcgt gcctcagctc ggcccgctgg gcgatcgcac
     6661 ccaggccatg ctggcgatca gcctgcacgc caccaacgac accctgcgcg acgtgctggt
     6721 gccgctgaac aagaagtaca atctcgaaca gctgatggcc ggcatccgcg cctatccggg
     6781 cctgtccaac gcgcgccggg tgacgttcga atacgtcatg ctcaagggcg tcaacgacag
     6841 ccccgccgag gcccgcgaat tggtcaagct gatcgacggc attcccgcca aggtgaacct
     6901 catcccgttc aacccctggc ccggcaccga ctacgaatgc tccgattgga agaccatcga
     6961 ggtcttcgcc gccatcctca acaaagccgg cttcgcctcg ccgatccgga ccccgcgcgg
     7021 gcgggatatt ctggcggcgt gcggacagtt gaagtccgag agcgagaagc tgagggcgag
     7081 cgcgatcagg aaagctgagc aggcgacagt cgaggcggct taagcggctg gcaggcttaa
     7141 ggcttggcta gccagccata cgcctcgacg cccacgatgg ttcgtcgaaa tccttgacgc
     7201 gggcaacggc taccaagtca cggccttcct cgtcttcacc ctcgcgatgt tccacgacca
     7261 cgatgtcgcc gggctgaaaa ctccaggcct catcttcggg gaccagctca tcaccaatgc
     7321 ggtaagtagt ttcgccgaga cgttcggcct gcaccggccg ccagacgtcg acgccttcgc
     7381 ccagcaggcg gacgtgaatg acgaccgtat cagtagatga agccatcgcc gccgacatgg
     7441 atcacgtctc ccgagacgtc gtcgatcacc acggatcggc ccgtcaccgg atgaatatag
     7501 cgcgttgcgg ggcgtcctgt ctcactgttg acggtcgcac ggctttcgcc gtggtcgcga
     7561 gcctcgtcga tctgctcgga tgtccagccg cgagccgcca tctgtcgtga tagccgggcc
     7621 ggtgacttca ccatgcccgg gcgtcgatca cgaccggtaa tccccgttga tctcgatgta
     7681 gcccttggtc aggtcgcagg tccagacggt ggcggaggcg cggccggagc cgacgtcgac
     7741 ggtgatgtcg atctcggggt tcttcatata ggcggtcatc gcggcctcgt cgtaggtcgg
     7801 cgacggcgcg ccgtcgatag cggctatgtt gggtccgaag cggatcgcca gtttgtcgcg
     7861 gtcgacgggt tcatcggtct tgccgacggc catgacgacc cggccccaat tggcgtcctg
     7921 accggcgatg gcggtcttga ccagggggct gtcggcgatc gatttggcca gcttgcgggc
     7981 tgacgccggg ctggtggctc catccaccgt gaccttgacg aacttcgtcg caccctcgcc
     8041 gtcgcggacc aactggtgag cgagatcgag catgacgcgg tccagcgcgg ccgagaaatc
     8101 cttcagccgc cgatcgccga cccgaccaat gcgaggcgca gtcgaggtgc cggtggcgaa
     8161 catcagacag gtgtcgttgg tcgaggtgtc gccgtcgacg gtcaccgagt tgaaggtcgt
     8221 gcggacgtgc aggcccagca gcgcgcgcag gacgctgggg tggatgtcgg cgtcggtgac
     8281 gatgaaggcc agcatggtcg ccatgtccgg cgcaatcatg cccgagccct tggcgatgcc
     8341 ggcgatacgg accttctgcc cctcgatctc gcactcggcg taggagccct tggggaaggt
     8401 gtcggtggtc atgatgccgc gcgcggcgag cgcccaggcg tcggagtcca gacggctttc
     8461 gatcgcaggc aactgagcgg cgatcttgtg gtcgtccagc accacgccga taacgccagt
     8521 cgaggccagc atcacgtcgc gctggcggca gccgaaccgt ttggcgacct cggaggcggt
     8581 gcggcgagcg gcgtcagccc cggccttgcc ggtgaaggcg ttggcgcaac cggcgttgac
     8641 caccagcgcg cgcacgtcct tgccgccctc gtcggagtcg agatggcgct tgcaccagtc
     8701 gacgggcgcc gagcccacct tgtgcttggt gaagaccccg gcgcaggtcg tgccgcggga
     8761 gaagcggaac accaccagat cgtcgcgctc gtgtttgtag aagcccgcgc gggccgttgc
     8821 gatctcgact ccgccgatcg gcgggatatg ggggaagggc acagccagcg gcgagatcgc
     8881 aaggccaggc ttgcccgggg tcgccggggc ggcgggcgca ggcgtcggag tggtggcgcg
     8941 ttttaatgcc gaggcgaagg gatccagcgc cttctcgatc gcatgctcga tcttctggcc
     9001 tgtggacgtc ctcgctgcag ttccggacgg aatgggcggc ttgttcatga tttcggcgct
     9061 ccagttgcgg gcgtcagcgc ggcggtcggg ggagacgtgc tgggagcggc agcaggcggc
     9121 gccgatgcag gctcctgctc gccgcccgtc gcgggttcgt tggccagcag gatatcgacc
     9181 ttggccttgc cgcgaagctg ttccaacaac tgccgcacgc cttcataggt caggtagcgc
     9241 acgatctgcg gtcgcgcctg ttccagcgtc ggcgggctct ccttgcgccg gtcctcgacc
     9301 cgcagaacgg cccagccacc ctcggtctgg aacggtccaa cggtcgaccc cgccggagcc
     9361 tcccgcagcg cattggcata ggcctgcggc atgacgtcga ccgtcgaata gccgaggtcg
     9421 ccgcccgaaa accgcgtcgc ctcgtcgatc gaccgctcct gagcgacggc agagaacgtc
     9481 gcgccctggg ccagaatacc gatgacggcg tccgcctcgg gcttggtgcg tgacaggatc
     9541 aggcggacac ggatttcctc cgaggtcttc gccagcctca gctgctcctg atacagggtc
     9601 tgcacggcct gatcgttgat gcggccgttg acgacgttct cgaccagcat gtcgccgagg
     9661 atccgctcct gcgtcgcctt cagccgccgt tgggccagag ctgagttatc cagcccgcgc
     9721 cgttcggcct cgcgcgccag cagtttctgg tccacgacct cgtccagcac gcgccggaac
     9781 aggtccgacg tgacgtccag cggctcgccc tccccgacca gtccttgcgc caccgcctcg
     9841 cgcttgacgt cggaggccca gatcgtctgg tcctgcacct tggcgaccgc gcgatcaccc
     9901 ggttctggcg gacggtctgc atcgctgcca cgaccacagg cggcgaccgc cagaagggcc
     9961 gcgaaagcga tgatcgtcag ggggttgcgc gaaagacgcc ttgcacgaaa aacggacaaa
    10021 gggggctact ccgtcccggg ctggaatgcc cctcgcgttg acaggggtta ggccggtgct
    10081 taagtcccgg ctgcgcccaa gtcgaggggt tttcgatcgt tcgcgcggcc ctcgcgcgcg
    10141 ccttgttcag gcgcaccggg ccgccgtctg atctggcccc tcttgcggcg ctgtcgccgc
    10201 ctcgtccgga acgtttccgg acactgttca gagccttcag acgctcgacg ccttcggatc
    10261 gctaatgctc ggcttcgcca agaaattttt cggctcttcc aacgaccgca aggtcaagga
    10321 tttcctcgtc cgcgtggaga agatcaacgc gctcgagccg aagttcgccg ccctgagcga
    10381 cgacgaactg cgcatgatga cccagacgtt ccgggatcgt ctggcgggcg gagaaaccgc
    10441 cgacaagatc ctcaacgagg ccttcgccgt cgcgcgtgag gcttccaagc gcgttcttgg
    10501 ccagcgtcag tacgacgtgc agctggccgg cggcatgatc ctgaacgagg gcggcatcgc
    10561 cgagatgcgc accggtgaag gcaagacgct cgtcgccatc gcccccgcct atctgaacgc
    10621 gctgggcggc aagggcgtcc acgtcatcac cgtcaatgac tacctcgcac gtcgtgacgc
    10681 cgaatggatg ggtcgcgtct atcgcttcct cggcatggaa gtcggcgtca tcgtcaacgg
    10741 cctgtcgtcc ggccagcgac aggcggccta tgccgccgac atcacctacg gcaccaacaa
    10801 cgaatttggc ttcgactacc tgcgcgacaa cctcgtctac gaccgcaacg agatggtcca
    10861 gcgcccgcac aatttcgcga tcgtcgatga agtcgactcc atcctgatcg acgaagcccg
    10921 cacgccgttg atcatttcgg gcccgaccga ggaccgttcg gatctatata aaatcctcga
    10981 cggcgtggtg aaggacctga tccaggacaa gaccaccttc gacctcgacg agaagcagaa
    11041 gcaggccctg ctgaccgagg aaggctccga gaagatcgag cagatcctcg aggacggcgg
    11101 tcatttcgcc gaggacacga cgggtctcta tgacgccgcc aacatcagcc tggtccacca
    11161 catcaaccag gctctgcgcg ccaacaccct gtatgcgcgc gacaaggact acatcatcaa
    11221 gggcggcgag atcgtcctga tcgatgagtt caccggtcgc atgatgacag gtcgccgcct
    11281 gtccgaaggc ctccaccagg ccatcgaagc caaggagggc gtgcagatca tgcccgagaa
    11341 ccagaccctg gcctcggtca cgatccagaa ctatttccgc ctctacgaaa agctgtccgg
    11401 catgaccggt acggccgcga ccgaggccca ggaattcggc gacatctaca agatggacgt
    11461 gctggaagtt ccgaccaacc gcccggtcca gcgcatcgac tacgacgacg aggtctatcg
    11521 caccgagcgc gagaagaacc tcgccatcgc caaacagatc gcggagtgcc acgttcgcgg
    11581 ccagccgatc ctcgtcggca ccgtctcgat cgagaagtcc gagcttctgt cggcgatgct
    11641 cgacacctac gaatacaaga ccgaggtctc gcgcaccctc aagccagagt tcgcgggcaa
    11701 gcccaaggaa gccgagaagc tcggtgacgc ggcttacgac atcgtctgga agtccgagtt
    11761 caccggcatt ccgcacgccg tcctgaacgc gcgccagcat gaacaggaag ccttcatcgt
    11821 cgccgacgcc ggcctgccgg gcgccgtgac catcgccacc aacatggccg gccgcggtac
    11881 tgacatccag ctgggcggta acctcgagat gcgtctcgag aagtggcgtc tcgaccagcg
    11941 caacacgggc gttatggtct cgcccgagga cgagaaggcc gaagaagttc gcctcaaggc
    12001 cgacatcgct gtgcacaagg ctacggcgct cgccgccggc ggtctgttcg tgctcggcac
    12061 cgaacgccac gaaagccgcc gtatcgataa ccagctgcgc ggtcggaccg gccgtcaggg
    12121 cgaccccggc acgtccaaat tcttcctctg ctgcgaggac gacctgctgc gcatcttcgc
    12181 cggcgaccgt ctggactcga tcatgcgttc gttcggcgtg gccgagggcg aggcgatctc
    12241 gcacccctgg ctgaaccggg ccgtcgagac cgcccagaag cgcgtcgaga cccgcaacta
    12301 cgacatccgc aagaacctgc tgaaatacga cgacgtcgtg aacgaccagc gcaaggccgt
    12361 gttcgagcag cgtcaggagt tcatggacgc cacggacctg tccgaactgg tcggcgagtt
    12421 ccgtcaggac gtcatcaagg acctggtcga ccgctacatg ccgcccaagg cctatgcgga
    12481 acagtgggac atcgacggcc tcgacgagaa ggtccgctcg acgctggggc tcgaactgcc
    12541 gctgcatgag tgggctgctg aagaaggcgt gtccaacgag gaggtcgagg aacggcttca
    12601 ggccgccgcc gcagcccgcg ccaccgaacg tctcgaactg gtcggcgagg gccagactcg
    12661 cggcatcgag aagcagttcc tgatgcagca gatcgatatg tcctggcgcg agcatctggt
    12721 ccatctggac cacctgcgcg gcgtcatcgg cctgcgtggc tacggccagc gcgacccgct
    12781 gaacgaatac aagaccgagg ccttctccct gttcgagaac ctgcttcagg agctgcgtca
    12841 caacgtgacc cgctggctga tgacggtcga gttccgcttc gaggccccgc cgtcgatgga
    12901 gatgccggag ttccgggaga tccacctcaa ccccggcacc ggcgagaacg agatgggcaa
    12961 tccgtacgcc cagaaccccg aacagcagtt ccagggcgac gaccgctcac gcctgccggt
    13021 cgagctcctg cccgtcggct gggaacgcac ctcacgcaac gccgactgcc cctgcggctc
    13081 cggccgcaag ttcaagcatt gccacggcag tctgatctag ggcggtcgtc actcggcagg
    13141 cgcatggctg ccatgccgcc ggtcgtggtg gcgtcgctat aggtcttccg tcctgcggaa
    13201 gcggtgggaa tgtttggaac cggcgccaca gcagcgacgg gcgcctgaca gatgcttgag
    13261 acgtgtgcct ttgcgaggct tttggaaact ccgacccgtg aattggcgtc ttgaccgatg
    13321 ggaggtcgcg ggcggatggt cgcggtaaca agacctccaa ggcctgtgga aacgccatga
    13381 ccaatccgat cgatcgccgt gacctgctgg tcggcgccgc cgcgagccta agcttcaccg
    13441 cctctcccgc cttcgcttcg cccgtaagtg acaccggtgt cgtcgcccgc accacggcgg
    13501 gccgtatcag tggctttcga gacggggata tcgccgtgtt caagggcgta ccctatggcg
    13561 ctgataccgc ccggacccgg ttcgctgcgc caaaaccgcc caagccatgg cgggccgtgc
    13621 ggccttgcac cgagttcgga gcgcccgcac ctcagggggg cggcaatccc ggcgcgggtt
    13681 atctgccgga tagcccctgg acgcaggatc agacgtccga ggactgcctg aacctccacg
    13741 tctggacgcc gtcggtcgac aaggcgaagc ggccggtgct ggtctggttc catggcggcg
    13801 ggttctccgg ctggaccgcc aacagtccgc tctatgacgg cgccaatctg gccaagtggg
    13861 gcgacgtggt cgtggtgacc gtcaaccacc ggctcaacgc gtttgggtat ctgtatctgg
    13921 cggagttggg cggtgaggcg ttcgcagact cgggcaacgc cgggatgctc gatctggtgc
    13981 tggcgttgcg ctggatccgg gacaacattg ccgaactggg cggtgacccc gaccgggtga
    14041 ccctgttcgg acagtcgggc ggcggggcca aggcctcggt gctgatggcc atgcccgccg
    14101 cgcgcggcct gttccaccgc gtcatgacca tgagcggtca gcaggtcacg gtcgttccgc
    14161 ccgagatggc gacgaccgcg acgcgcaaat tcctgaccaa cgccggggtc gagacgcccg
    14221 aggggctcct gcgtttgtcg aaggaccagt tgatcgcggc cgggcggggc gtgtccacgg
    14281 ctccggttct ggacggccga tcgctgatcc gcgatccgtt cgagcccgac gcgacgccgc
    14341 tttcgcgcga cgtgccgctg atcatgggca acacccatga cgagacgcgc ttcctgatcg
    14401 ggcttgacga ccccgccctg ttcgacctga cgtgggaaca gctgctgccg gcgctgaagc
    14461 gcagcatccc gaacttcttt gggccgttgt cgcccgaggc gacgctggtc tggtatcgcg
    14521 acaagcaccc ctcgtattcg ccgagccaga tcttcttcgc catgacgacc gagctgcggt
    14581 cctggcgggc gcaggtgatc caggccgacc ggcgcgccgc tcagccgggg gcgaatacct
    14641 gggtctatca gttcgactgg cagagtcccg tgatgggcgg aaatctgggc gcgccccatt
    14701 gcggcgacat cccgttcttc ttccgcaatc accgcgagac gccgaccatg accggcggcg
    14761 gaccggagac cgatgtcgtc gcggatacga tgagctcggc gctggtgaac tttgcgcgga
    14821 ccggaaatcc caatggcaga ggcgtgccgg agtggccgga atacgagctg ccgggccggg
    14881 tgaccatgca gtggaatacg gtcagcaagg cgacgcccga tccacgcggc gaagagcgac
    14941 ggctgctggg gttgatcccg tacaggcagc cggggacgtg aggcgttacg ccgagcgttg
    15001 cttcagtggc gcggagccgt cggacaggct ctcgttgaga tagacctcgg cctcctgcgc
    15061 cggcagggcg ggggcatagc cgaagccctg gccatagtgg cagctggcgt cgagcaacag
    15121 cttggccagg ccggcgttct cgaccccttc ggcgacgacc tcgaggttga ggtcacgacc
    15181 aaggttgacc accgacttga cgatcttggc cgagccctcg tccttgtcca tcgtcagtac
    15241 gaaatagcgg tcgatcttga gggtgtcgaa cggcaggcgc gccagatagg acagcgacga
    15301 aaaaccggtg ccgaaatcgt c
//
LOCUS       DASVTM010000377         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_460380,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000377 DASVTM010000000
VERSION     DASVTM010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460380"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VF633_10275"
     CDS             complement(<1..1404)
                     /locus_tag="VF633_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8471488.1"
                     /translation="MTSNTMTRALLAGASAAALLASLALTPTKAWAEPAVAAPQDAPG
                     MGPAQDPEAASVDDVIVTGVRQALATSLEIKKNADTFVDSITATDIGAFPDKSAAEAL
                     ARVPGITVNRLQSADDSTHPSGEPTGVLIRGLTQVRTEFNGRDSFSADSARGLQFNDV
                     SPELLAGVDSYKNQTAAMIEGGLAGTVNLRTRVPFDQNGLLLTGNLKANYGDRSGEWT
                     PEYSALISNTWDTSVGRFGALASFARSDVITRTESVIMDKINTYCSAGFGTADLAALD
                     ADGQVTCSANPFGGTGFAYAPDGIRFSQVDYDRQRRGLAGALQYENNAGTFAATVQYL
                     ESTYRNAWLERASHVKLDGNYYGTRAFNPRSGSSLIGSSGLTFGSDGMLQSGLLTQPH
                     GSFSGTYESLQAAINAGSAVPGLPFVNYCGNGVCGANPRNGLYIENEARNFDHDEGTR
                     DLSANFRWDPTDRLRLTA"
     gene            complement(1566..>3071)
                     /locus_tag="VF633_10280"
     CDS             complement(1566..>3071)
                     /locus_tag="VF633_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471489.1"
                     /translation="LEERLAPDPADDRTGSDPRALGPMASASAGLFGIQIVWGLQSVT
                     TSRIFQSLGAEMADLPLLWIAAPVTGLLIHPLIGWLSDRTHSPYGRRRPYIAVGTVLT
                     AAAMIFTGSANTLSAAVLALWLLTFSVNVAMQPMRSLIADIVPQAQLPRAYAVQVLFI
                     GGGAIFASCLPWILAQWALIDPQPLGVRASWRLAYFVGAAALLASMGWTLAKTREPVG
                     AEKAVAPTAPITARPLRPLFWVVIGLAIAGGAAALGLRREVYLLASVFLAYGALQAIV
                     LALRRRHPGRPVRGVLEIVLCIGAMPPVMRQLAAVQFFTWFALFTAWVYAVPTVAALQ
                     FGNPPPGSASYETAADWVGVLFGVQDAVAIVVALCLPRIARVIGVARCHAACLAVGAL
                     SFVAMSLAPGWGLLALPWAGFGIAWASILSLPYAIVASAGPAERLGVNLGVHNIFLVL
                     PQLVGASVLGLVVQGVFHGRVGLILPLAALSFLAAAILAARLPRQAQPSWG"
BASE COUNT          554 a          971 c         1067 g          479 t
ORIGIN      
        1 ggccgtcagc cgaagtcggt cggtcggatc ccatcggaag ttggccgaca gatcgcgcgt
       61 gccctcgtcg tggtcgaagt tccgggcctc gttttcgatg tacagaccgt tgcgcgggtt
      121 ggccccgcag accccgtttc cgcagtagtt gacgaagggc aggccaggta cggccgaacc
      181 cgcgttgatc gcggcctgaa ggctctcata agttccgctg aagcttccgt gaggctgcgt
      241 cagaagtccg gactgaagca tgccatcgga gccgaaggtc aggccggagc tgccgatcaa
      301 cgacgatccc gaccgcgggt tgaaggcgcg cgtgccgtaa tagttgccgt cgagtttcac
      361 gtggctggcg cgctccagcc aggcgttgcg ataggtggac tcaagatact ggactgtcgc
      421 tgcgaaggtg cctgcattgt tctcatactg gagcgcgccg gcgagaccgc ggcgctgccg
      481 gtcgtaatcg acctgcgaga agcggattcc atccggcgca taggcgaagc ctgtgccgcc
      541 gaacggattg gcgctacagg tcacctggcc gtccgcatcg agggccgcca gatccgctgt
      601 gccgaagccg gcggaacagt aggtgttgat cttgtccatg atcacgctct cggtgcgcgt
      661 gatcacatcg ctgcgcgcga agctggcgag cgcgccgaag cggccgaccg aggtgtccca
      721 ggtattgctg atgagagccg aatattcagg ggtccattcg cccgaccggt cgccgtagtt
      781 agccttgagg ttgcccgtga ggagcaggcc gttctggtcg aacggcacgc gcgtccgcag
      841 attaactgtg cccgcgaggc cgccctcgat cattgctgcg gtctggttct tgtaggaatc
      901 cacgccggcc agaagctcgg gcgagacgtc gttgaactgc agaccgcggg cggagtcggc
      961 cgagaagctg tcgcggccgt tgaactcggt tcgaacctgg gtcagccccc ggatcagcac
     1021 gccggtcggt tctccggacg ggtgggtcga gtcatcggcc gactggagcc ggttgaccgt
     1081 aatgccggga acgcgcgcaa gcgcctcggc cgcagatttg tcggggaagg cgccaatgtc
     1141 ggtggcggtg atcgaatcaa cgaaggtgtc cgcattcttt ttgatctcca gggaggtcgc
     1201 caaggcctgg cggaccccgg tgacgatcac atcgtcgacc gaagccgcct ccggatcctg
     1261 cgccgggccc attccgggag cgtcttgggg cgctgccacg gcgggttccg cccaggcttt
     1321 tgtcggcgtg agcgcgaggc ttgcgagcag ggccgcggcc gaggcgcctg ccaacagggc
     1381 gcgcgtcatc gtattgctgg tcatgtagtc tccctcccat tccacgcctt ggtgcgcggt
     1441 tatcgcatta tcttgcgtaa gattgcacaa atccccggcg cgtcaagtcc cgctgtgcaa
     1501 gcctcctgtg gcattcgctt acggattgcg gtcgaagact gaagtctcca atgcaatatc
     1561 ttgcattagc cccagctggg ctgcgcttga cggggcaggc gagccgccag gatggcggcg
     1621 gccaggaagg acaaggccgc cagcggcaga atgaggccta cccgtccgtg gaatacgccc
     1681 tgcacgacca gcccgaggac gctggccccc acgagctggg gcagaaccag gaaaatgtta
     1741 tggacgccca ggttcacgcc gagacgctcc gcaggcccgg cgctcgccac gatcgcgtag
     1801 ggcagggaca gaatggaggc ccacgcgatg ccgaagccgg cccacggaag cgcgagcagg
     1861 ccccaccctg gcgcaaggct catggcgacg aagcttaggg ccccgactgc cagacaggcg
     1921 gcgtggcacc gggcgacgcc gatcactctt gctatccggg ggaggcacag ggcgacgacg
     1981 atggcgaccg cgtcctggac gccgaacaga acgcccaccc agtcggccgc cgtctcgtag
     2041 gaggcggatc cgggcggagg attgccgaac tgaagggcgg ccacggtcgg aaccgcatag
     2101 acccaggccg tgaagagggc gaaccaggta aagaactgca ccgcggcgag ctgacgcatg
     2161 accggcggca tggcgccgat gcacagcacg atctcaagga cgccccgcac gggacgcccg
     2221 gggtggcggc gccgcagcgc cagaacgatg gcttggagcg cgccataggc caggaatacg
     2281 ctggccagca gatagacttc gcgccggagt cccagagcgg ccgcgccgcc tgcgatcgcc
     2341 aggccgatca ccacccagaa gaggggtcga agcgggcgag cggtgatcgg cgccgtcggc
     2401 gctacggcct tctcggcgcc gaccggctcg cgggttttgg ccagggtcca gcccatgctg
     2461 gcgagcagcg ccgccgcgcc gacgaaatac gccagcctcc agctggctcg cactcccagg
     2521 ggctgtgggt cgatcagcgc ccactgagcc aggatccatg gcaggcagga ggcgaagatg
     2581 gctccacccc cgatgaataa aacctgcacc gcataggctc gaggcagctg tgcctggggg
     2641 acgatgtccg cgatcaggga gcgcatgggc tgcatcgcga cattgactga gaaggtcagg
     2701 agccagagcg cgagcacggc cgcggagagc gtgttcgcgc tgcctgtgaa gatcatcgcg
     2761 gcggcggtga ggacggttcc cacggcgatg taagggcgac gccgaccgta agggctgtgc
     2821 gttcgatcgc tgagccagcc gatgagagga tggatcagca gcccggtcac tggcgccgcg
     2881 atccacagga gcggcagatc cgccatctcc gcgcccagag actggaagat gcggctcgtc
     2941 gtgacgctct ggagccccca cacgatctgt atgccgaaca ggcccgcgct tgcgcttgcc
     3001 atggggccaa gagcgcgagg gtcggaaccg gtccgatcgt cagccgggtc tggcgccaac
     3061 ctctcctcca a
//
LOCUS       DASVTM010000378         5346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_37002, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000378 DASVTM010000000
VERSION     DASVTM010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37002"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1155
                     /locus_tag="VF633_10285"
     CDS             166..1155
                     /locus_tag="VF633_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8471490.1"
                     /translation="MIAFLTQPQNLLSILVGLLAFATLVTLLGAFNTGAKLEDRMKAV
                     AVRRDELKRRSRQALAKEGVLRHSDTGFHKDVVEKLNLAKMLEDPKVADKLAQAGFRG
                     PKPMNTFYFFRFATPFVFFAIVGFYLFVLNDFGQPVMFRVTATMAAAVMGFYAPNIYL
                     TNRIAKRQLSIMQAFPDALDLLLICVEAGMSIEGAIQKVSQEIGGTSIDLAEELSLLS
                     AELSYLPERRQAYEGLAKRTNHAGVKSVATAMVQAEQYGTPLGTALRTMAKENRDLRM
                     SAAEKKAAALPAKLTVPMIIFFLPVLFIVILGPAVITIQDTMAKNQKPAATAQ"
     gene            complement(1142..1591)
                     /locus_tag="VF633_10290"
     CDS             complement(1142..1591)
                     /locus_tag="VF633_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8471491.1"
                     /translation="MDTTRIDNRIGVKATAETIWSLLSDLESWSRWNPVETEAEGKIA
                     FGGSISVTEAIPGLPERRVTGKIGDWQPYAQLVWRESRGWQFTAMRYYEIEELAPGSC
                     IVSNGIIFGGMRGEGFHDKHRGAIKAAYARIGEALREASEALAATAQ"
     gene            complement(1664..2959)
                     /locus_tag="VF633_10295"
     CDS             complement(1664..2959)
                     /locus_tag="VF633_10295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471492.1"
                     /translation="MSPRSPAFIMLCTVSLIASAGNMALQAVLPAIGRQFQLADTLVV
                     GVFAISALMWTVASPVWARLSDRLGRKRVILIGMAGFSASMAGFGLAATSGLEQWLGP
                     VMAFVLMGVARTIYGVFGSAAPIASQAYVADRTAPAERTQAMSLLASAQGLGTVIGPT
                     LAPLLILPVVGLAGPMYAFSLVAAIAFVWVLKKLPSGGVTRVPSSTGQPDHDGRGLWK
                     DSRVRDFLLYGLLVTGAQAINISVLGFHVIDEMAETGLTTVQAQPFIAVAMFAGAAGT
                     LMVQWGLIPLLRLQPRALMRWGAGLALAGNVLSILTPGFYGVVVGYAVVSMGVGFARP
                     GFTAGASLAVGPGEQGAVAGLMMSIAGISFLFPPVIGVALYQLAAFAPFAANAVLMIS
                     ALLVCWWSASLQSAGVSVNIGVEATSEPPASQPGPEGNR"
     gene            complement(2963..3334)
                     /locus_tag="VF633_10300"
     CDS             complement(2963..3334)
                     /locus_tag="VF633_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471493.1"
                     /translation="MASKPVQITKTQWKDVVLVCKKCSKKLDGGFGPDGDQSLKKALK
                     RYLKPPKSANVGKGRKAELVIKQTDCFDICPKNAVIAVNAANPKALLIVPVGADLLEV
                     KARLGLDDGRRTRPNLTLIGD"
     gene            3429..4760
                     /locus_tag="VF633_10305"
     CDS             3429..4760
                     /locus_tag="VF633_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8471494.1"
                     /translation="MPRFKSRLTAFVMTSALALGAAAAHAQTAPDTLATDVAAAAAQI
                     NPSVIEWRRHLHQNPELGFAEVETAAYIAEHLRAMGLEVRTGVGKTGVVAILRGGLPG
                     NHVVALRTDMDALPVLEATGEPFSSTATGTYMGATVPVAHACGHDAHMAMLLGAAEVL
                     SKMNDRIPGTIVFIFQPAEEGAPPGEPLGGAALMIKEGVLNDPKVEAIFGLHVVPGKT
                     GTLFYRPQGFMAASDRIDITLHGKQTHGAWPWRGVDVIAATSQVIETINTLTARTVDP
                     TVHPTVFTISTLNAGVRYNIIPDQSVLSGTLRTFDIASRDDLVKRAEAAIDHVAAIYG
                     ATADFAIKQNAALTYNDPALSTWLAPVLEEAAGAGNVNPASPPTTVAEDFSYFQSEIP
                     GVFYHLGGTKIGEDPATTAPNHSPGFDVDESVLPVGVRAHVMTAMRFLQRP"
     gene            4869..5210
                     /locus_tag="VF633_10310"
     CDS             4869..5210
                     /locus_tag="VF633_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8471495.1"
                     /translation="MITRLEPGARMSEAVIHGDTVYLAGQVGEPGDDIVAQTTTVLSE
                     IDRLLALAGSDKSKILMATIWLADIADFAAMNSVWDAWVDQANPPARATSEARLATPD
                     YLVEIIIIAAK"
BASE COUNT         1003 a         1883 c         1578 g          882 t
ORIGIN      
        1 ccgttgtgat gtgcctcgtc tacataacga ctcccgccta catcaccctg ctgttcaccg
       61 atccgcgtgg tcaggtgatg ttgctgggcg cgggcgtgtg gatgggcctg ggaattttcg
      121 tgatgaaaaa aatgatcgcg ttcaagttct aggctggaaa ctgaaatgat cgcttttctt
      181 acccagccac agaacctgct cagcatcctc gtcgggttgc tcgccttcgc caccctcgta
      241 acgctgctgg gcgccttcaa caccggcgcc aagctggagg atcgcatgaa ggccgtcgcg
      301 gttcggcgcg acgaactgaa acgccgctcg cggcaggcgc tggccaagga aggcgtcctg
      361 cgccacagcg acaccggctt tcacaaggat gtcgtcgaga agctgaatct ggccaagatg
      421 ctggaagatc cgaaggtcgc ggacaagctg gcgcaggccg gtttccgtgg gccgaagccg
      481 atgaacacct tctatttctt ccgcttcgcg accccgttcg tgtttttcgc catcgtcggg
      541 ttctatctgt tcgtcctcaa cgacttcggc cagccggtga tgtttcgcgt cacggcgacc
      601 atggctgctg ctgtgatggg cttctatgca cccaacatct atctgaccaa ccgcatcgcc
      661 aaacgccagc tgtcgatcat gcaggctttc cctgacgccc tcgacctgct gctgatctgc
      721 gtggaggccg ggatgtcgat cgagggtgcg attcagaagg tgtcgcagga aatcggcggg
      781 acctcaatcg atctggccga ggagctcagc ctgctgtcag ccgaactcag ctacctgccc
      841 gagcgtcgcc aagcctatga gggcctggca aaacgcacca accacgccgg tgtgaagtcg
      901 gtcgccaccg ccatggttca ggccgagcaa tacggaacgc cactcggtac ggccctgcgg
      961 acgatggcca aggaaaaccg cgacctgaga atgtccgccg cggaaaagaa agccgccgcc
     1021 ctgccggcca aactcaccgt gcccatgatc atcttcttcc tgccggtgct tttcatcgtg
     1081 atcttgggcc cggcagtcat caccatccag gacacgatgg ccaagaacca gaagcctgcg
     1141 gctactgcgc agtagccgcg agagcttcgg acgcctcccg caaggcctcg ccgatccggg
     1201 cataggctgc cttgatcgct cccctgtgtt tgtcgtggaa gccttccccg cgcatgccgc
     1261 cgaagatgat cccgttagag acgatgcagc tgcctggcgc cagttcctcg atctcgtaat
     1321 agcgcatggc cgtgaactgc cagccccggc tctcgcgcca gaccagctgg gcataaggct
     1381 gccagtcgcc gatctttccg gtcacccgcc gctccggcag gccggggatc gcctcggtca
     1441 cagagatcga tccgccaaaa gcgatcttgc cctctgcttc cgtctcgacg ggattccacc
     1501 ggctccagga ctcgaggtcg ctgagcaggc tccagatcgt ctcggcggtg gccttcaccc
     1561 cgatgcggtt gtcgatgcgc gtcgtgtcca tcaccgggat ttgccacggc tgtttgacgg
     1621 aaggaagacg gcttgaatgt cggagcttct ggaaacagcc gactcagcgg ttgccttcgg
     1681 ggcctggctg agatgcgggc ggctccgacg tggcctcgac cccgatattg accgagacgc
     1741 ccgcgctctg cagcgacgcg ctccaccaac agacgagcaa ggccgagatc atcaacaccg
     1801 cattggcggc aaacggcgcg aatgcggcca gttgatacag ggcgacgccg atgaccggcg
     1861 ggaacaggaa gctgatgccg gcgatggaca tcatcaaccc cgcgaccgcc ccctgttctc
     1921 ctggcccgac cgcgagcgag gctcctgctg taaacccggg tcgggcgaaa ccgacgccca
     1981 tcgacaccac cgcatagccg accaccacgc cataaaatcc cggcgtcagg atgctgagaa
     2041 cattgcccgc cagcgccagc cctgcgcccc accgcatcag ggcgcgcggc tgcaacctga
     2101 gcagcgggat cagcccccac tggaccatca gcgtccctgc cgcgcccgca aacatggcga
     2161 cggcgatgaa gggttgagcc tgaacggtgg tgagccccgt ctcggccatc tcgtcgatga
     2221 cgtgaaagcc aagtaccgag atgttgatcg cctgcgcgcc cgtaaccagc aggccataga
     2281 gcaggaagtc gcgtacgcgg ctgtctttcc acaaacccct gccatcatgg tccggctggc
     2341 cggtcgagga aggcacgcgg gtcacgccgc cactcggcag cttcttcaac acccagacaa
     2401 aggcgatcgc cgccaccaac gagaaggcgt acatcggtcc tgctaatccg acgaccggca
     2461 ggatcagcaa cggcgccagc gtcgggccga tcaccgtgcc caaaccctgc gccgacgcca
     2521 gcaaggacat ggcctgcgtc cgctccgccg gagccgtccg atcagccaca taggcctgcg
     2581 aggcgatcgg ggcggcagaa ccgaacacac cgtagatcgt ccgcgccacg cccatcagca
     2641 cgaaggccat gaccgggccg agccactgct caagccccga agtcgcggcc agcccgaacc
     2701 cggccatcga ggcgctgaac cccgccatgc cgatcagaat gacgcgtttg cgcccgagcc
     2761 gatccgacag tcgcgcccac accggcgagg ccaccgtcca catcagggcc gagatggcga
     2821 acacgccgac gaccagcgta tcggccagct ggaactgccg cccgatggca ggcaacaccg
     2881 cctgcaacgc catgttcccc gccgaggcga tcaggctgac agtacacagc atgataaagg
     2941 ccggtgagcg cggcgacatc ggttagtcgc cgatcagggt caggttcggt cgcgttctcc
     3001 gcccgtcatc caacccaagc cgcgccttga cctccagaag gtcggcgccg acaggcacga
     3061 tcagcagggc cttggggttg gcggcgttga ctgcgatgac cgcattcttg ggacagatgt
     3121 cgaaacagtc cgtctgcttg atcaccagct cggccttgcg gcccttgccg acgttcgcgc
     3181 tcttgggcgg cttcagatag cgcttcaacg ccttcttcaa gctctggtcg ccgtcgggcc
     3241 cgaagccacc gtcgagcttc ttggagcatt tcttgcagac gaggacgacg tccttccact
     3301 gggtcttcgt aatctggacg ggcttgctgg ccataaaccc gagatcgctg actcgcgtcg
     3361 ccgacgcaag cgccttgcgc gatccgtcgc ctaagcttca tgatcgaaca tcgctcccgg
     3421 agaagctcat gccccgtttc aagtcgcgcc tgaccgcgtt cgtgatgacc tcggccctgg
     3481 cgctgggagc cgctgcggct cacgcccaga cagcgccgga cactctcgcc accgacgtcg
     3541 cggctgcagc ggcgcagatc aatcccagcg tcatcgaatg gcgccgccac ctgcatcaga
     3601 accccgagct gggcttcgca gaggttgaga ccgccgccta tatcgccgaa cacctgaggg
     3661 ctatggggct ggaggtccgc actggcgtcg gcaagaccgg cgtggtcgcc atcctgcgcg
     3721 gaggcttgcc gggcaaccac gtcgtcgccc tgcgcacgga catggacgcc ctgcccgtgc
     3781 tcgaagcgac cggtgagccg ttctcctcga ccgcgaccgg gacctatatg ggcgcgaccg
     3841 tgccggtcgc ccacgcctgt ggccacgacg cccacatggc catgctgctg ggcgccgccg
     3901 aggttctgtc gaagatgaac gaccgcatcc ccggcaccat cgtcttcatc ttccagcccg
     3961 ccgaggaagg cgcgcctccg ggtgaaccgc tgggcggcgc cgccctgatg atcaaggaag
     4021 gcgtgctgaa cgacccgaag gtcgaggcga tcttcggcct gcatgtcgtg cccggaaaga
     4081 ccggcacgct gttctatcgg ccgcagggct tcatggccgc atccgaccgc atcgacatca
     4141 ccctgcacgg caaacagacc catggagcct ggccgtggcg cggcgtggac gtgatcgcgg
     4201 cgaccagtca ggtgatcgag acgatcaata ccctgaccgc ccgcacggtc gacccgaccg
     4261 tccacccgac ggtcttcacc atcagcacac tcaacgccgg cgtccgctac aatatcatcc
     4321 cggaccagtc ggtgctgtcg ggcacgttgc gtaccttcga catcgcctca cgcgacgatc
     4381 tggtgaagcg cgccgaggcc gccatcgacc acgtcgccgc catctatggc gccaccgccg
     4441 atttcgccat caagcagaac gccgccctga cctacaacga cccggccctg tcgacctggc
     4501 tggccccggt gctcgaagag gcggcaggcg cgggaaacgt caatccggcc agccccccaa
     4561 ccaccgtggc ggaggacttc tcctatttcc agagcgaaat cccgggcgtc ttctaccacc
     4621 tcggcggcac gaagatcggc gaggaccccg ccaccaccgc ccctaaccac tcgcccggtt
     4681 tcgacgtcga tgaaagcgtc ctccccgtcg gcgtccgcgc ccacgtcatg accgcgatgc
     4741 ggttcctgca acggccgtaa tcctccccac cctgtgggga gggggaccgc ccgccgcttg
     4801 cgggtggtgg aggggctgac gcggcgccgt ccatcggcta agcccccctg accttcagga
     4861 gctttcccat gatcacccgt ctcgaacccg gcgcccgcat gagcgaagcg gtgatccatg
     4921 gcgacaccgt ctatctggcc ggtcaggtcg gcgagcccgg cgacgacatc gtcgcccaga
     4981 ccacgaccgt cctgtccgaa atcgaccgcc tgctcgccct cgccggttcg gacaagtcaa
     5041 agatcctcat ggccaccatc tggctggccg acatcgccga cttcgccgcc atgaacagcg
     5101 tctgggacgc ttgggtcgat caggccaacc cgcccgcccg cgccacttcc gaagccaggc
     5161 tggccacgcc cgactacctc gtcgaaatca tcatcattgc tgcaaaatga gcgacaccga
     5221 actcgaacgt ttcaaggccc agcgcgtcac cgcgatctac cgcctcgacc tgatcgagaa
     5281 aggcgccaag atcacctacg acgacggcac gccggtcgac atggcctccg aaaaggcccg
     5341 ccttca
//
LOCUS       DASVTM010000379        12695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_783372,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000379 DASVTM010000000
VERSION     DASVTM010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783372"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF633_10315"
     CDS             <1..320
                     /locus_tag="VF633_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX8471496.1"
                     /translation="LDAKLLGLDFEVYAIVKLMLPSSENLNIFEAAVSEWPEVVQCAT
                     ITGREDYVLRIVTSDMHAFDKFLREKLLSLAIVSDCESHIVTRGVKNVTALPLGIITP
                     HVK"
     gene            359..1294
                     /locus_tag="VF633_10320"
     CDS             359..1294
                     /locus_tag="VF633_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8471497.1"
                     /translation="MIEMVIDARRKDLGGFEVGRVLPFHSRRMIGPFIFLDQMGPAEF
                     APGSDAINVRPHPHIGLSTLSYLFEGEIMHRDSLGYTQAIRPGEVNWMTAGKGIVHSE
                     RTDPLKKATGGPMHGMQAWIALPNEAEDMDPGFYHHGEADLPAYENGGLFARLVAGEA
                     YGASSAVQTSSPLFYVHWEMKEGVRTAPPPGKGAGGMSERALYVAKGQIEVAGRTFHE
                     GQLIVLEPHAEPTVKSLTQSTVMALGGEPVGERLIWWNFVASSQERIDQAKADWKAGR
                     MALPTEDDLEFIPLPDVPSTPEAAPAPVPGPTDPV"
     gene            complement(1301..2275)
                     /locus_tag="VF633_10325"
     CDS             complement(1301..2275)
                     /locus_tag="VF633_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8471498.1"
                     /translation="MQTRIIKTDGLNQQILEAGEGSLVLLIHGFPELGVSWRAQVEAL
                     ANAGYHAVAPDMRGYGGTDKPERTEDHSILNLVGDMVDLVRALGEKTCVVVGHDWGAP
                     VAWHCALMRPDVFRAVVGLSVPFQPRIPKGAPTPMMAALARRAGLGDLYINRFQPADA
                     HEPFDVDPATTLRKLFWSYDGATPDADASGGGQSTGFLPHNVSMLDSIDDAATLPPWM
                     TEAHFAEYVEAFTAGGFKGPIDWYRCIDLNWSQTAFLQDKRIAQPSLFVVGERDPVRH
                     YAGQHEAGLKTWLTDLRGQIVVPGAGHWLQQERPDEVNAALLEFLRGL"
     gene            2343..3179
                     /locus_tag="VF633_10330"
     CDS             2343..3179
                     /locus_tag="VF633_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8471499.1"
                     /translation="MTSLPDGTLVPVLGQGTWEIGDDPRKADAEQAALARGLDLGLTL
                     IDTAELYGNGKSERLVGEVIKGRRDEVFLVSKVRPENAGEMSMLLSCERSLERLGVER
                     LDLYLLHWESRHRMDEIIAGFEELVDEGLILRWGVSNLDIKAMTILEGLDGAEHCQTN
                     QVLYNLGSRGVEFDLMPWSQERNMPLMAYSPLGRGGAGGLLEHPALVEIADRHAVSTA
                     QIALAAVLRQEGVIAIPKASTAAHVEANAEALDIALDSEDMAVLDRAFPAPATAQPLD
                     II"
     gene            3617..3976
                     /locus_tag="VF633_10335"
     CDS             3617..3976
                     /locus_tag="VF633_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471500.1"
                     /translation="MNQGVYLMNIRNLVMACATIAMLVPAAASAQTEAVSFSIDNQTD
                     KIIAMVYYGHASASEWSDDILSGVIEPGETLEVTVDDDTSDCMYDFMYTFEDGSDFVE
                     RVNMCEIDGETFEFTGG"
     gene            complement(4053..6269)
                     /locus_tag="VF633_10340"
     CDS             complement(4053..6269)
                     /locus_tag="VF633_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8471501.1"
                     /translation="MNMQSFENFKIDIADGIATVAFDVPNKSMNTLTGAVLKELPVLV
                     EALKTDETVKGVVLTSGKASGFCAGADLGDMAGGVLAGGGDLQKAYDAGWSINAAFRS
                     LETMGKPVAAAINGLALGGGLEFTLACHYRVAGDNPKIQLGLPEIKVGLFPGGGGTQR
                     LPRLIGVQAAMMAMTEGKSFRPNDAKGAGIIHEVVPAGTEVEAAKAWIKGGGKAVAPW
                     DAKDFKLPGGGPYHPAGIQNFLVGNAMIKKQTYGNYPAALNLMKAVYEGVQVPIDAGL
                     RIETRYFIKTLMTPQAQAMIRSLFLSKQELDKGAVRPAGIPKSDPKKVTVIGAGMMGA
                     GIAYVQALAGIETILIDQTQEAADKGKAHVEELLKKRLSRGQIDQAKFDALLGSVTAT
                     TDYELIKGSDLVVEAVFENREIKADVTRRAEAQLVEGAIFGSNTSTLPITGLSEASVR
                     PEDFIGIHFFSPVDKMMLVEIILGEKTGQEAIAKTLDYVLKIKKTPIVVNDSRGFYTS
                     RCFSTFLMEGMAMLDEGYAPALIDNVGRMTGMPRGPLEMHDDVALDLSYKIAKQTKLD
                     LGDKYVPNAGEALVATMVEGGRFGRKNGKGFYDYPAEKGSPKTLWSGLTELAPTTKAN
                     DKAEEPSALAYIDELKTRLLYRQAVEVARCWEEGVIDDPREADLGAILGWGFAPWTGG
                     PITLIDQTGLAKFVETADRLAATYGDRFKVPQLLRDMAAKGETFYGNFAGAQKAAA"
     gene            complement(6281..7486)
                     /locus_tag="VF633_10345"
     CDS             complement(6281..7486)
                     /locus_tag="VF633_10345"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8471502.1"
                     /translation="MADAYIYDAVRTPRGKGKKDGSLHEITGLSLATQVLEALRDRND
                     LDTSKVDDVILGCVTPVGEQGADIARTAVLNAGWSQYTAGVQVNRFCASGLEAVNMAT
                     AKVKSGEADFAVGGGVEAMSRVPMGSDGGAWPVDPSSAFQTYFVPQGVSADMIASKYG
                     FSRDDVDAYSMESHKRAAAAWADGRFSKSVIPVKDQLGLVRLDRDETIRPNTDMQTLG
                     SLNPSFAMMGQMAFDAVISQRYPEVETLNHVHTPGNSSGIVDGSAGVLIGTLEAGQAL
                     GLKPRAKILGGASIGSEPSIMLTGPEYVTRKLLGKLGMTPDDIDVWELNEAFAAVVLR
                     YMQALNIDHSKMNVNGGGISMGHPLGATGAMITGIALDELERSNKSTALITLCIGGGM
                     GTATVIERV"
     gene            7752..7970
                     /locus_tag="VF633_10350"
     CDS             7752..7970
                     /locus_tag="VF633_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471503.1"
                     /translation="MRIGHHGYEIQLYRRRENWLWVVFDDDGDETRSGHAADHTIALA
                     QARRAVARLRSPFAAWLEQLLPFPANHA"
     gene            8144..9325
                     /locus_tag="VF633_10355"
     CDS             8144..9325
                     /locus_tag="VF633_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8471504.1"
                     /translation="MGVYNRAPLEVERGRGARLWSKDGTEYLDCVQGISTNGLGHAHP
                     ILVQAVKDQAEKLWHVSNIFKIPGQEALADMLCDHSFADMVFFTNSGTEAVECALKTA
                     RRYHWANGAPERIDIYGFDGSFHGRTYGAINAAANPSYTEGFGPPMEGFHQLVWGDRE
                     ALEAAFRSPTAAAIVLEPVQGEGGCRVTPDEDLRWMRQMADETGTLLIFDEIQCGMGR
                     TGKLWAHEWAGMEPDIMTIAKALGGGFPIGACLTTQKVGAAMTVGAHGSTFGGNPLAM
                     AVGIAAFGELSKPEVIAHVGEISAYLKQQLVGLQERFPDVIVDVRGKGLLVGLKLVPN
                     NREFMAIARDEQNLLIAGGGDNCVRILPPLNMTIDEAREAVQKFEGACEFVRSKTTAA
                     A"
     gene            9326..10240
                     /gene="argF"
                     /locus_tag="VF633_10360"
     CDS             9326..10240
                     /gene="argF"
                     /locus_tag="VF633_10360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX8471505.1"
                     /translation="MVRHFLDIHRLSAEDLRGILDDAHARKAARKGWPQGRVDADAPA
                     KDHVLAMIFEKNSTRTRFSFDAAIRQLGGASIIATASDMQLGRGEPIEDTAKVLSRMV
                     DAVMIRANNHEDVERFARVSTVPVINGLTDRSHPCQILADLQTIEEQLGPIAGKTIAW
                     IGDGNNVCHSFMHAAPKFGFHLNVACPAEYHPDLHDLAKGGNSVTLTSDPREAVLGAD
                     VVVTDTWVSMGDGDYEERLQAFETYTVDEALMALADPEAVFLHCLPAHRGEEVTDAVI
                     DGPKSLVWDEAENRIHAQKAVLAWCFAG"
     gene            complement(10330..10833)
                     /locus_tag="VF633_10365"
     CDS             complement(10330..10833)
                     /locus_tag="VF633_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8471506.1"
                     /translation="MADDTLSPADAEQEFWKHLKSSNTGMLGIDTPGYHSQPMTAYRE
                     EETGTIWFFTRDDTDLARDVAVGGQKGMFTYGSKDQEVWACFHGSMSISRNQAVIDKH
                     WNPIVAAWYADGKDDPHLTLIKFDGDDGRVWISKKGAVGFFFEVAKANLTKTMPNVGG
                     VADVNLS"
     gene            complement(10905..11111)
                     /locus_tag="VF633_10370"
     CDS             complement(10905..11111)
                     /locus_tag="VF633_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471507.1"
                     /translation="MPLSLTLIFLAIGVVVTLFAGWRGARPTDLMKGPRMIPWRFLML
                     LSGAVSFLLVVHLAALFGLTQPGR"
     gene            11339..>12695
                     /locus_tag="VF633_10375"
     CDS             11339..>12695
                     /locus_tag="VF633_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471508.1"
                     /translation="MSAAAPWSVKGIDPKAREVAKDLARRSGMTLGEWLNTMIMDDVE
                     DEEIVVPLSRRAHASETIDRRGRSRRLDDAYEPDPYPRDSGYDRSSETLQRVAASVDA
                     IAARLEAAERRSTVAIQGVDQAVTGLVRRLDGADKETRTYGRRIDDISEELKEGHRRL
                     RRFEQEMGPETKATFGKVESGLGALASRLYDIEERQRTSLNELRQRMDAAEKTVGPGA
                     GTDLLAQVGARLDTAQARTTEALKGLERSFAGLDQRMRAAEGRVEPEGSREAARFEKL
                     AETLGRQVDASRAEMMRRLDTAEHGGRMERIERAMAAIGEQARTSEQRSAEGIDAMGR
                     EVLRIAQNLNGRLQATDARSQATDARATASDARVLAMDAAIQRIDRRVEQVETNAADK
                     IARAGQAIAADLTASLNEKLDRDMGRYAQAVEHRLNRTDDQHALALEKLGGEITRISD
                     RL"
BASE COUNT         2315 a         3993 c         4133 g         2254 t
ORIGIN      
        1 tgctggacgc caagctgctg ggcctggact tcgaggtcta cgccatcgtc aagctgatgc
       61 tgccgtcgtc cgagaacctg aacatcttcg aagccgctgt gagcgaatgg cccgaggtcg
      121 tgcagtgcgc caccatcacc ggccgcgagg actatgtgct gcgcatcgtc acatcggaca
      181 tgcatgcgtt cgataaattc ctgcgtgaga agctgctgtc gctggcgatc gtctcggact
      241 gcgagagcca tatcgtgacg cgcggtgtga agaacgtaac cgcgcttccg ctcggcatca
      301 tcactcccca cgttaagtaa acagcgcgcg ttagacgacc gaactcaggg gatacgacat
      361 gatcgagatg gtgatcgacg cgcggcgcaa ggacctcggg ggcttcgagg tcggtcgcgt
      421 gctgccgttc cattcgcgcc ggatgatcgg gccgttcatc tttctcgacc agatggggcc
      481 ggcggagttc gcgcccggct cggacgcgat caacgtccgc cctcacccgc acatcggcct
      541 gtcgaccctg agctatctgt tcgagggtga gatcatgcac cgcgacagcc tcggctatac
      601 gcaggcgatc cgtccaggcg aggtcaactg gatgacggcg ggcaagggca tcgtccactc
      661 cgagcgcacg gatccgttga agaaggcgac tggtggcccg atgcacggga tgcaggcctg
      721 gatcgccctg ccgaacgagg ccgaggatat ggatccgggc ttctatcacc acggcgaggc
      781 cgacctgccc gcctatgaga acggcggcct gtttgcacgg ctcgtcgcag gcgaggccta
      841 tggcgcatct tcggcggtac agacgtcgtc gccgctgttc tacgtccact gggaaatgaa
      901 ggagggcgtg cgtacggcgc cgcctccggg caagggcgca ggcgggatgt ccgagcgcgc
      961 tctctatgtg gccaagggcc agatcgaggt cgcgggtcgc accttccacg aaggccagtt
     1021 gatcgtgctg gagccccacg ccgaacccac cgtaaaatcc ctgacccaat cgacggtcat
     1081 ggcgctgggc ggagaaccgg tgggcgaacg gctgatctgg tggaatttcg tcgcctcgtc
     1141 gcaagagcgg atcgatcagg ccaaggccga ctggaaggcg ggccgcatgg ctctgccgac
     1201 cgaggacgac ctcgagttca tccctctgcc cgacgtgcct tcgacacccg aggccgcgcc
     1261 ggcgcccgtc cccgggccga cggatccggt ctagccagcg tcagaggccg cgcaggaact
     1321 cgagcaaagc cgcattgacc tcgtcaggcc gctcttgctg cagccagtga ccggcgccgg
     1381 ggacgacgat ttgtccgcgc aggtcggtga gccatgtctt gagacccgcc tcgtgctggc
     1441 ccgcgtaatg acgcacgggg tctcgctcgc cgacgacgaa gagtgacggc tgggcaatgc
     1501 gcttgtcctg cagaaacgcc gtctggctcc agttcaggtc gatgcagcga taccagtcga
     1561 tcggaccttt gaagccgcct gcggtgaacg cctcgacgta ttcggcgaaa tgagcctcgg
     1621 tcatccacgg gggcagagtt gcggcatcgt cgatgctgtc gagcatcgaa acattgtgcg
     1681 gcagaaagcc ggtcgactgg ccgccgcctg aggcgtccgc gtcgggcgtc gcgccgtcgt
     1741 aggaccagaa cagcttgcgc agcgtcgtcg cgggatcgac gtcgaacggt tcgtgggcgt
     1801 cggcgggctg gaagcggttg atatagaggt ctccgagacc ggcgcgcctg gccagcgccg
     1861 ccatcatcgg cgtcggagcg cccttgggaa tgcgcggctg gaacggcact gacagcccga
     1921 cgaccgcgcg gaatacatcg ggtcgcatca gcgcgcagtg ccaggcgacc ggcgcgcccc
     1981 agtcgtgacc gactacgacg caggtctttt cacccaacgc ccggaccaga tcgaccatgt
     2041 cgccgaccag attgaggatc gaatggtctt cagtgcgctc cggcttgtcg gtcccgccgt
     2101 agccgcgcat gtcgggcgcc acggcatgat agccagcgtt cgccaaggcc tcgacctgcg
     2161 cgcgccaact gacgcccagc tcggggaagc cgtggatcag cagcaccagc gacccttcac
     2221 cagcctccag aatctgttgg ttcaggccgt cggtcttgat gatcctggtc tgcattctgt
     2281 cctccgcgac gaacatcttt ggcgccgggg gcggcgtcgt gcattcttgg gtcatgacac
     2341 gcatgacttc gcttccagac ggtacgttgg ttccggtgct cggccaaggg acgtgggaga
     2401 tcggtgacga tccccgcaag gccgacgccg agcaggcggc gctcgcacgc gggctggatc
     2461 tgggcctgac cctgatcgat accgccgagt tgtacggcaa cggcaaatcc gagcggttgg
     2521 tcggggaggt catcaagggg cgtagggacg aggtgtttct cgtttccaag gttcggcctg
     2581 agaacgccgg cgaaatgtcg atgctgctgt cctgcgagcg cagtctggag cggctcgggg
     2641 tcgagcggct ggacctgtac ctgctgcatt gggaaagccg ccaccggatg gacgagatca
     2701 tcgccggttt cgaggaactg gtggacgagg ggctgatcct tcgctggggc gtgtccaacc
     2761 tcgacatcaa ggccatgacc atcctcgaag gactcgacgg cgctgagcac tgccagacca
     2821 atcaggtgct gtataatctg ggctcgcgcg gcgtggagtt cgacctgatg ccatggtctc
     2881 aggagcgcaa catgccgctg atggcctatt caccgctcgg gcgcggagga gccggggggt
     2941 tgctggaaca tccggcgctg gtcgagatcg ccgatcgaca tgccgtctcg acggcccaga
     3001 tcgccttggc tgcagtcctt cgtcaggagg gcgttatcgc catcccgaag gcctcgaccg
     3061 cggcccacgt tgaagccaac gccgaggcgc tggatatcgc cctggactcc gaggacatgg
     3121 ccgttctcga ccgggccttc ccggcgccag ccacggccca gccactcgac atcatctaag
     3181 cttccccagc gtcaataaaa cagcccgggt tgggcggacg cactggattc tcacgcaaaa
     3241 ttcgtcccaa tcttgagacg atggcgcgtc agagcagcag cgcagctttc gttcagattg
     3301 cgttcacgcg attaacccca tgcctgagtg cagtgcgctg atcgcccttg tcggacgcga
     3361 tccgaggggg gactcgggac tgaatcggtc gggcgatcgg aggcgtcggg ggacgtggcc
     3421 ggcaccgctt gattgaagat cggctcaaac cggtcgaatg agacggtggc cttgtcgggg
     3481 gacagggccg ccgtctttat ttaaatcttc aatataaatt cgctggccat ctaatatacc
     3541 gtcgccaaag aatgtttctt tcgtgttcat attcagttca gggaaacgtg cgcattgagg
     3601 cgtcagagca ttctgcttga accagggggt ctaccttatg aatatccgca atctcgtcat
     3661 ggcctgcgcc acgatcgcca tgcttgtccc agctgctgcc tcggcacaga ctgaagccgt
     3721 ctcgttttcg atcgacaacc agacggacaa gatcatcgcc atggtctatt atggtcatgc
     3781 gtcggcaagc gaatggagcg acgacatcct gtccggcgtg atcgagcccg gcgagacgct
     3841 ggaagtgaca gtcgatgacg acacgtccga ctgcatgtac gacttcatgt acaccttcga
     3901 ggacggcagc gacttcgtcg agcgcgtgaa catgtgcgaa atcgacggtg aaaccttcga
     3961 gttcaccggc ggctgatcgg tctccggacc tgaatgcaga acggccccgg gatcgctccc
     4021 ggggccattt tactatgcat agtcagcccg gatcaggcgg ccgccttctg cgctccggcg
     4081 aagtttccgt agaacgtctc gcccttggcc gccatgtcgc gcagcagctg cggaaccttg
     4141 aagcggtcgc cgtaggtggc tgcgaggcga tcggcggttt ccacgaactt cgccagacct
     4201 gtctggtcga tcaaggtgat cggtccgccg gtccagggcg cgaagcccca gccgaggatg
     4261 gcgcccagat cggcttcgcg aggatcgtcg attacgccct cttcccagca gcgcgcgacc
     4321 tcgacggcct gacgatacag caggcgagtc ttcagctcgt cgatataggc gagcgccgaa
     4381 ggctcctcgg ccttgtcgtt ggccttggtg gtcggagcca gttcggtcag gcctgaccac
     4441 agcgtcttgg gcgaaccctt ttcagccggg tagtcgtaga agcccttgcc gttcttgcga
     4501 ccgaaacgac cgccctcgac catggtcgca accaaggcct cgccagcgtt cgggacgtat
     4561 ttgtcgccaa ggtcgagctt ggtctgcttc gcgatcttgt agctgaggtc cagcgcgaca
     4621 tcgtcgtgca tttccagcgg gccgcgcggc atgccggtca tgcgcccgac gttgtcgatc
     4681 agagccggag cataaccctc gtccagcatc gccatgcctt ccatcaggaa ggtggagaag
     4741 cagcgcgagg tgtagaagcc tctggagtcg ttgacgacga tgggcgtctt cttgatcttc
     4801 aggacgtagt cgagcgtctt ggcgatcgct tcctgaccgg tcttctcgcc gagaatgatc
     4861 tcgaccagca tcatcttgtc gacgggcgag aagaagtgga tgccgatgaa gtcctcggga
     4921 cggaccgagg cctcggacag gccggtgatc ggcaaggtcg aggtgttgga gccgaagatg
     4981 gcgccttcaa ccagttgagc ctcggcgcga cgggtgacgt cggccttgat ctcgcggttc
     5041 tcgaacacgg cttcgacgac gaggtccgag cccttgatca gctcatagtc ggtcgtagcg
     5101 gtgaccgagc cgagcagggc gtcgaacttg gcctgatcga tctggccgcg cgacaggcgc
     5161 ttcttcaaca gttcctcgac gtgagccttg cccttgtcgg cggcttcctg ggtctggtcg
     5221 atcaggatgg tctcgatgcc ggccagagcc tgaacgtagg cgatgcctgc ccccatcatg
     5281 ccagcgccga tgacggtgac cttcttcgga tccgacttgg gaatgcccgc cggacgcacg
     5341 gcgcccttgt cgagctcctg cttggacagg aacagcgagc ggatcatcgc ctgggcctgc
     5401 ggcgtcatca gggtcttgat gaagtagcgg gtctcgatgc gcagaccggc gtcgatcggc
     5461 acctgaacgc cttcgtagac ggctttcatc aggttcaggg cggccggata gttgccgtag
     5521 gtctgcttct tgatcatggc gttgccgacc aggaagttct ggatgcccgc cgggtgatag
     5581 gggccgccgc cgggcagctt gaagtccttg gcgtcccacg gcgcgacggc cttgccgccg
     5641 cccttgatcc aggccttggc ggcctcgact tcggttccgg cagggacgac ttcatggatg
     5701 atgcctgcgc ccttggcgtc gttcggacgg aaggacttgc cttcggtcat ggccatcatc
     5761 gccgcctgca cgccgatcag gcgcggcaga cgctgggtgc cgccgccgcc ggggaacagg
     5821 ccgaccttga tctcgggcag accgagctgg atcttcggat tgtcgcctgc aacgcggtag
     5881 tggcaggcca gcgtgaactc gagcccgccg ccgagggcga ggccattgat cgcagcggcg
     5941 accggcttgc ccatggtttc gagcgagcgg aaggcggcgt tgatcgacca gccggcgtca
     6001 taggccttct gcaggtcacc gccgccggcg agtacgccgc cagccatgtc gccgaggtcc
     6061 gcgccggcgc agaagcccga ggccttgccc gacgtcagga cgacaccctt gaccgtttcg
     6121 tcggtcttga gggcctcgac cagcaccggc aactctttga gcaccgcgcc ggtgagggtg
     6181 ttcatcgact tgttggggac gtcgaaagcg accgtggcga tgccgtcggc gatgtcgatc
     6241 ttgaagttct cgaaggattg catgttcatt ctctccggga tcagacgcgt tcgatgacgg
     6301 tggcggtgcc catgccgcca ccgatgcaca gggtgatcag ggcggtcgac ttgttggacc
     6361 gctccagctc gtccagggcg atgccggtga tcatggcgcc ggtggcgccg agcgggtggc
     6421 ccatcgagat gccgccgccg ttcacgttca tcttcgaatg gtcgatgttc agggcctgca
     6481 tatagcgcag aacgacggcg gcgaacgcct cgttcagctc ccagacgtcg atgtcgtcgg
     6541 gggtcatgcc cagcttgccg agcagcttgc gggtcacata ctccggaccc gtcagcatga
     6601 tcgagggctc ggaaccgatc gaggcgccgc cgaggatctt cgcacgcggc ttcaggccga
     6661 gagcctgacc ggcctcgagc gtgccgatga ggacacccgc cgaaccgtcg acgatgccgg
     6721 aagaattgcc cggcgtgtgg acgtggttga gggtctcgac ttcgggatag cgctggctga
     6781 tcacggcgtc gaacgccatc tggcccatca ttgcgaacga cggattcagg ctgccgagcg
     6841 tctgcatgtc ggtgttgggg cggatcgttt cgtcacggtc gagacgcacg aggccgagtt
     6901 ggtccttgac cgggatcacc gatttcgaga accggccgtc ggcccaggcg gccgcagcgc
     6961 gcttgtggct ttccatcgaa taggcgtcga cgtcatcgcg gctgaaaccg tatttcgacg
     7021 cgatcatgtc ggccgaaacg ccctgtggca cgaaataggt ctggaaggcc gatgacggat
     7081 cgaccggcca tgcgccgccg tccgagccca taggcacgcg gctcatggct tcgacgccgc
     7141 cgccgacggc gaaatcggcc tcgccggact tcaccttggc ggtcgccatg ttcacggctt
     7201 cgaggcccga ggcgcagaag cggttgacct gaacgcccgc cgtatattgc gaccagccgg
     7261 cgttgagcac ggcggtgcgg gcgatgtcgg cgccctgttc tcccaccggg gtgacgcagc
     7321 caaggatgac gtcgtcgacc ttggaggtgt ccagatcgtt acggtcgcgc agggcttcca
     7381 gcacctgtgt cgccaggctc agcccggtga tctcgtgcag cgatccgtcc ttcttgccct
     7441 tgccgcgcgg ggtgcgcacc gcgtcataga tataggcgtc agccatggga ctcagtctcc
     7501 aataaggacg gttcgaagcg tgctggccat gaaccctacg tttacgtcaa cgtcaagtaa
     7561 gaaggcggcg acctgaagct agtctggaga tcaattttcg cctccagaca caccggcctc
     7621 gcctccaaag gtggcgccat aatcatcaag ctgtgccgaa aacgtccaaa attgaggcgt
     7681 ttccgttgtg gcgccgtcgt tgcttacagg ggcaacatag agccgttatg gccactccac
     7741 gggcgggaac gatgcgtatc ggccatcacg gctacgagat tcaactctat cgccgccgtg
     7801 aaaactggct gtgggtcgtg ttcgacgacg acggtgacga gacgagatca ggccacgcgg
     7861 cggatcacac tattgcgctc gcacaagccc ggcgcgccgt ggcgcggttg cgcagcccct
     7921 tcgcagcctg gctggaacag cttcttccct tccccgccaa ccacgcctga tcattgcgca
     7981 cctgagtcca cgccagactg tcaggaaagc ttcaccggcg ggcccgggcg cggtgttgac
     8041 acaccccctc ccccgtccga caagtccgct ttgagaaagt cccctcgcat cccgcgtcgg
     8101 ggcttcattg cttttggagg tatgttccgt gtccagccat ctgatgggtg tctacaatcg
     8161 cgcgccgctg gaagtggaac gcgggcgagg cgcacgtctg tggagcaagg acggcacgga
     8221 atacctcgac tgcgtacagg gcatctcgac taacggtctg ggacacgccc acccgatact
     8281 ggtgcaggca gtcaaggatc aggccgagaa gctctggcac gtctccaaca tcttcaagat
     8341 tcccggtcag gaagccctcg ccgacatgct gtgcgatcac agcttcgccg acatggtgtt
     8401 cttcaccaac tcgggcaccg aggcagtcga gtgcgcgctg aagaccgcgc ggcgttatca
     8461 ttgggccaac ggcgcgcctg agcggatcga catctacggc ttcgacggct cgttccacgg
     8521 ccggacctat ggcgcgatca acgcggcggc gaacccgagc tacaccgaag gcttcggccc
     8581 gccgatggaa ggcttccacc agctggtctg gggcgatcgc gaagcgttgg aagccgcctt
     8641 ccgctcaccg acggcggcgg cgatcgtgct ggagcctgtt cagggcgagg gcggttgccg
     8701 ggtcacgccc gacgaagacc tgcgctggat gcgccagatg gccgacgaga ccggcaccct
     8761 gctgatcttc gacgagatcc agtgcggcat gggccggacc ggcaagctgt gggcgcatga
     8821 atgggccggc atggagcccg acatcatgac catcgccaag gcgctgggcg gcggcttccc
     8881 catcggcgcc tgcctcacca cgcagaaggt cggtgcggcc atgacagtcg gcgcccatgg
     8941 ctcgaccttc ggcggcaacc cgctggccat ggcggtcggc atcgcggctt ttggcgaact
     9001 gtcgaagcct gaggtcatcg cccacgtcgg ggagatttcg gcctatctga agcagcaact
     9061 ggttggcctt caggagcggt tcccggacgt catcgtcgac gtgcgcggca agggcctgct
     9121 ggtcggactg aagctggtcc cgaacaatcg cgagttcatg gccatcgccc gcgacgagca
     9181 gaatctgctg atcgccggcg gcggcgacaa ctgcgtgcgc atcctgccgc cgctgaacat
     9241 gacgatcgac gaggctcgcg aagccgttca gaaatttgag ggcgcgtgcg agttcgtgcg
     9301 gtccaagacg acggcggccg cataaatggt ccggcatttc ctcgacattc accggctgtc
     9361 cgccgaagat ttgcgcggca tcctcgacga cgcccacgcc cgcaaggcgg cccgtaaggg
     9421 ttggccgcaa ggcagggtcg acgccgatgc gccggccaag gatcatgtgc tggcgatgat
     9481 cttcgaaaag aactctaccc ggacccgctt cagcttcgac gccgccattc gccagttggg
     9541 cggagcctcg atcatcgcca cggcgtccga catgcagttg ggtcgcggcg agccgatcga
     9601 ggacaccgcc aaggtgctgt cgcgtatggt cgacgccgtg atgatccgcg ccaacaatca
     9661 cgaggacgtc gagcgcttcg cccgcgtctc gaccgtgccg gtgatcaacg gcctcaccga
     9721 ccggtcgcac ccgtgccaga tcctggccga ccttcagacc atcgaggaac aactcggtcc
     9781 catcgccggc aagacgatcg cgtggattgg agatggcaac aacgtctgcc acagcttcat
     9841 gcatgcggcg ccgaagttcg ggtttcacct gaacgtcgcc tgcccggccg aatatcatcc
     9901 ggacttgcat gacctggcaa agggcggaaa ctccgtcacc ctgaccagcg atccgcgcga
     9961 ggccgtgctg ggggccgacg tcgtggtcac cgatacctgg gtatccatgg gcgacggcga
    10021 ttacgaagaa cgattgcagg cgttcgagac atacacggtc gacgaagccc tgatggcgct
    10081 ggccgatccg gaggcagtct tcctgcattg cctgcccgcc catcgcgggg aagaagtcac
    10141 cgacgccgtc atcgacggac cgaagtcgct ggtctgggat gaagccgaaa accgcatcca
    10201 tgcgcagaag gccgtgctcg cctggtgttt tgcggggtag gtcagaaaag cgggcagtag
    10261 gcagataggc ataaggcagt aggaggaatc ttctccagat cgccggccct actgcctact
    10321 gcctactgcc tagctgagat tcacatccgc cacgccgccg acgttcggca tggtcttggt
    10381 caggttggcc ttggcgactt cgaagaagaa cccgacagcg cccttcttgg aaatccagac
    10441 gcggccgtcg tcgccatcga atttgatcag cgtcaggtgc gggtcgtcct tgccgtcggc
    10501 gtaccaggcg gcgacgatcg ggttccagtg cttgtcgatg acagcctgat tgcggctgat
    10561 cgacatcgat ccgtggaaac aggcccagac ctcctgatcc ttggagccat aggtgaacat
    10621 gcccttctgg ccgccgacag cgacgtcacg ggcgagatca gtatcgtccc gggtgaagaa
    10681 ccagatcgtg cccgtctctt cctcgcgata ggccgtcatc ggctgggaat gatagccggg
    10741 cgtatcgatg cccagcatgc cggtgttgga gctcttcagg tgcttccaga attcctgttc
    10801 agcgtcggcg ggggagaggg tgtcatcggc catgtggcgt tctttcctgt tgaaggctgg
    10861 aggaagaaca ggctgagccc ggcgcggttc cttgcatgac gccgtcaacg accgggctgg
    10921 gtaagaccaa acagcgcagc cagatggaca accagcagga agctgaccgc gccggacaga
    10981 agcatgagaa agcgccaagg gatcatgcga ggccccttca tcaaatccgt gggacgcgcg
    11041 ccccgccacc ctgcgaacag ggtcacgaca acgccgatgg cgaggaagat cagggtcaga
    11101 gaaaggggca caagtcccac tggcggcgga tggctttcac tgcaagtccg agcggccaaa
    11161 ttccttgccg aggcgttaat ctttacgggt cattaaccac gatcacgcga gaaaccgggt
    11221 tggggacaga cgatttccac aggaagatgg ctcacaccct acatcttgcg gttaacctta
    11281 gatttacacc ccggatatgg gcgactagcg tgacttccat gggtcgggga gcgaatcagt
    11341 gagcgcagct gcgccgtgga gcgtcaaggg catcgatccc aaggcgcgcg aggtcgccaa
    11401 ggatctcgca cgccggtcgg gcatgacgct cggcgagtgg ctcaacacca tgatcatgga
    11461 cgatgtcgag gacgaggaaa tcgtcgtccc gctgtcccgc cgggctcacg catccgagac
    11521 catcgatcgt cgtggccggt cacgtcgtct cgacgacgcc tacgaacctg atccctatcc
    11581 ccgtgacagc ggctacgatc gttccagcga gaccctccag cgcgtcgccg cctcggtcga
    11641 cgccatcgcc gcccgcctcg aggccgccga gcgtcgctcg accgtagcga tccagggcgt
    11701 ggatcaggcg gtcactggtc tggtgcgtcg ccttgacggc gccgacaagg agacccgcac
    11761 ctacggtcgc cgcatcgacg acatttccga agagctgaaa gaaggccacc gccgcctgcg
    11821 ccgcttcgag caggagatgg gtcccgagac caaggcgacc ttcggcaagg tcgaaagcgg
    11881 tctgggcgcc ctcgcctcgc gtctctacga cattgaagaa cgccagcgga ccagcctgaa
    11941 cgagctgcgc cagcgtatgg acgcggccga gaagacggtc ggcccaggcg caggaacgga
    12001 cctgctggcc caggtcggcg cgcgtctgga cacggcgcag gcccgcacca cagaggctct
    12061 aaagggtctg gagcgctcgt tcgccggtct cgaccagcgg atgcgcgccg ccgaaggccg
    12121 ggtcgagccc gagggctcac gcgaggccgc ccggttcgag aaactggccg aaacgctcgg
    12181 ccgtcaggtc gacgccagcc gcgccgagat gatgcgtcgg ctggacaccg ccgagcacgg
    12241 cggccggatg gagcggatcg aacgcgccat ggccgccatc ggcgaacagg ctcgcacctc
    12301 cgagcaacgg tcggccgagg gtatcgacgc catgggccgc gaggtcctgc gcatcgccca
    12361 gaacctgaac ggccgtctgc aggcgacgga cgcgcgcagc caggccacag atgcgcgcgc
    12421 cacggccagt gacgctcgcg tcctcgcaat ggacgccgcg atccagcgca tcgaccgtcg
    12481 agtcgaacag gtggaaacga acgccgccga caagattgcc cgcgctggtc aggctatcgc
    12541 cgcagacctg accgccagcc tgaacgagaa gctggaccgc gacatggggc gctacgccca
    12601 ggccgtggaa caccggctga atcgcaccga cgaccagcat gctttggcct tggagaaact
    12661 gggcggcgag atcacccgga tttcggaccg actgg
//
LOCUS       DASVTM010000380         5930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_885955,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000380 DASVTM010000000
VERSION     DASVTM010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885955"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VF633_10380"
     CDS             complement(<1..534)
                     /locus_tag="VF633_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8471509.1"
                     /translation="MIISRRAVLAASSAVMTTGGAVRAAGPAPAVVASTWDFGAAANA
                     GAIRARMAGGSAVDMVEAGAKVPEADVTNHSVGLGGYPDRDGKVTLDAVVMVHAGNVG
                     AVAALEDIVHAVSVARAVLEKTPHTMLVGPGARQFAIDQGFETTNLLTPEAEAAWKDW
                     LRTEHYAPRINSENSDWK"
     gene            complement(531..1268)
                     /gene="radC"
                     /locus_tag="VF633_10385"
     CDS             complement(531..1268)
                     /gene="radC"
                     /locus_tag="VF633_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX8471510.1"
                     /translation="MSETADTDTPRKVPPKHGGHRDRLRERAAKGGIDALPDYELLEM
                     LLFRSVPYKDTKPIAKDLLSRFGSLEGIGAASHEAIDDQVAKSLSIVRGKQSRATALD
                     LQLIYDVARRVAREPASKRPLISSWSALLAYVRVALQHEPREQFRVIYLDNRNHLILD
                     EVQNRGTIDHAPVYPREVIRRALELSSRNIIVVHNHPSGDPTPSRADIDMTQQIVAAG
                     HPLNVTVHDHLIVGRDGVASFKQLGLL"
     gene            1359..2120
                     /locus_tag="VF633_10390"
     CDS             1359..2120
                     /locus_tag="VF633_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX8471511.1"
                     /translation="MTQIAPSPTAAVLIIGDEILSGRTADTNLNTIARFLTALGIDLK
                     EARTVGDVPGQIVEALNALRAAHDYVFTTGGIGPTHDDITADCVAQAFGVPLSEHPEA
                     LAILGRRYGDDFNAARRRMARVPEGGSLIANPVTDAPGFQIGNVFVMAGVPKIMTAML
                     EDIAPRLRTGAVVHSMTLRVVGVGEGAIADTLRAAANANRELSFGSYPFGAGSDEEIG
                     TNLVIRGRDRDRIEVAVGELSIAISVFGIETVVVT"
     gene            2312..3613
                     /locus_tag="VF633_10395"
     CDS             2312..3613
                     /locus_tag="VF633_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX8471512.1"
                     /translation="MNASTVARAGGLTLTAALLASVATPTLAGSFYLQEQSARGAGRA
                     YSGEAADTGVSSMWWNPAAIARSGREVYVGAHGLLLDSDVDNAGSTITYPGGTTVPVQ
                     GQNHNGDPIESGIVPNFAISTPIGDRFAVGLAVEAPYNFTTKYQPGSFARYDALTSEL
                     RSANISLVGAMKVTDWLDIGAGFDAQYVEAKLTSALPNLSPLLPDGSSSLEGDGWDYG
                     WNVGAQAHTGPWQFGLSYRSEIEHELEGDVAISGLLGPIAAGNFSGNGTASFNTPWFA
                     SASVRYAVNDKLTLNAQVNRIGWSEFDAIRVRYTTGGDTFIQDYKDVTTGAVGFDYVA
                     NERVTFRGGVAYDPTPTQDGERTARIPDADRWLFSGGVSVKATDTMTVDTGLTYIAFS
                     DSDIASTHTSYAGTAAATTSNLRGTAEGYGIGASVGVRWAF"
     gene            complement(3778..5334)
                     /locus_tag="VF633_10400"
     CDS             complement(3778..5334)
                     /locus_tag="VF633_10400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8471513.1"
                     /translation="MTTTFESFGLNKALLQALASEGYTNPTPIQAQAIPDVMLGKDLL
                     GIAQTGTGKTAAFALPILHRLAENRVAPAPRTTRTLILSPTRELASQIAESFKTYGRH
                     LGFKVAVVFGGVKYGPQERAIQAGLDVLVATPGRLLDHMQQKTIDLSSTEIFVLDEAD
                     QMLDLGFVKPIRQIVSRIPAKRQNLFFSATMPSEIGKLAGELLKDPVKVQVTPQSTTV
                     ERINQSVIHIEQGRKRALLTEMFADDNYTRCLVFTKTKHGADKVAAYLEAGGVQAGAI
                     HGNKSQPQRERTLAAFKAGKLRVLVATDIAARGIDVDGVSHVVNFELPFVPEAYVHRI
                     GRTARAGADGSAISFVAGDEMKLLRDIEKVTRQKIPATDRRNDKSLGLLDQSIMASGV
                     GSKATMPEDGRGDRQDRGPRNPRRDGVKSEHGGQPHRVRKPRDGAGAGGGGGRDGGGQ
                     RDRTHHSDRGDRPMASTQPFDPLAADRARSAANAAARPAGVTKPDGEIQRRPRARRPS
                     NGGKGYVAKA"
     gene            complement(5615..>5930)
                     /locus_tag="VF633_10405"
     CDS             complement(5615..>5930)
                     /locus_tag="VF633_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX8471514.1"
                     /translation="LTTVTLSWCFIHLIFAQHYAHGFYGADDKGKDRGGLVFPGETEP
                     DYWDFIHFALIIGVACQTADVQISDRRLRRISSVHSVTAFVFNTVIVALGVNFAVSLL
                     GS"
BASE COUNT         1027 a         1902 c         1941 g         1060 t
ORIGIN      
        1 cttccagtcg ctgttttcgc tgttaatgcg cggtgcatag tgctcggttc gcagccagtc
       61 cttccacgca gcttcggcct cgggtgtcaa aaggttggtg gtctcaaacc cttgatcgat
      121 ggcgaactgg cgcgcgccgg gaccgaccaa catggtgtgt ggggtttttt ccagcacggc
      181 ccgggcgact gaaaccgcgt ggacgatatc ctccagcgcc gcgactgccc caacgttgcc
      241 cgcatgaacc atgaccacag catccagcgt cactttccca tcgcggtcgg gatagccgcc
      301 cagaccgacc gaatgattgg tgacgtcggc ctccgggacc ttcgcccccg cctctaccat
      361 gtccaccgcc gatccgcccg ccatgcgcgc ccgtatcgcc ccggcgttgg ctgctgcgcc
      421 gaagtcccag gtcgaggcca cgacggccgg cgccgggccg gccgcccgca cggctccgcc
      481 agtggtcatg acggcggacg atgcggccag aacggccctg cgagagatga tcacaggagt
      541 ccaagctgct tgaaactggc cacgccgtcg cggcctacga tcaggtggtc atgaacggtg
      601 acgttcaggg gatggcctgc ggcgacgatc tgttgcgtca tgtcgatatc agcccggctc
      661 ggtgtcggat cgcccgatgg atggttgtgt accacgatga tgttgcgcga cgacagctcc
      721 agtgcccgac ggatgacctc gcgcggatag acgggggcgt ggtcgatcgt gccccggttc
      781 tggacctcgt caaggatcag atggttgcgg ttgtcgaggt agatgacgcg gaactgctcc
      841 ctcggctcat gctgcaacgc gacccggaca taggcgagca atgcgctcca ggacgagatc
      901 agcgggcgct tcgacgccgg ttcgcgggcc acccgtcggg cgacgtcata gatcaactgc
      961 agatccagcg ccgtggcccg ggactgtttg cctcgcacga tggacagcga cttcgcgacc
     1021 tgatcgtcga tggcctcgtg ggacgcggcg ccgatgccct ccagactgcc gaaccgggac
     1081 agcaggtctt tggcaatcgg cttggtgtcc ttgtagggca cgctccggaa caacagcatt
     1141 tcgagcagtt cgtagtcggg cagggcgtcg atgccgccct tggccgcgcg ttcccgcagc
     1201 cggtcgcgat ggccgccgtg tttcggcggg accttcctgg gcgtgtcggt gtcggcggtt
     1261 tcgctcacgg cctgccttgc gggttcggcg aacggtagcg gttgtctggc cacgatcaag
     1321 cgccatgaca gctctgggtt ggaggattaa gggacggcat gacccagatc gcgccctcgc
     1381 ccacagccgc cgtccttata atcggcgacg aaatcctgtc cggacgcacg gcggacacca
     1441 atctgaacac catcgcgcgg tttctgacgg cgctggggat cgacctcaag gaagcgcgga
     1501 cggtcggaga cgtcccgggt caaatcgtcg aggcgttgaa cgcgctgagg gcggcgcatg
     1561 actatgtctt caccaccggc ggcatcggcc cgacgcatga cgacatcacc gccgattgcg
     1621 tcgcgcaggc gttcggcgta ccattgtctg aacaccccga ggccctcgcc atccttggcc
     1681 gacgctatgg cgacgacttc aacgccgcgc gcaggcgaat ggccagggtt cctgaagggg
     1741 gatcgctgat cgccaatccc gtaaccgacg cgccgggctt ccagatcggc aacgtgttcg
     1801 tcatggcggg cgtgccgaag atcatgactg cgatgctgga agacatcgct ccgcgcctga
     1861 gaaccggcgc cgtcgtccat tcgatgacct tgcgcgtcgt tggagtggga gagggcgcga
     1921 tcgccgatac gttgagagca gctgcgaacg ccaatcgtga gctttccttt ggcagctatc
     1981 ctttcggcgc aggttcggac gaagaaatcg ggaccaatct ggttattcgc ggaagagatc
     2041 gcgataggat cgaagttgcc gttggggaac tttcaatcgc gatttcagta ttcgggatag
     2101 aaacggtggt tgtcacctga agatgcggct ttcaaggcac agtctgtcct cgaagctgcc
     2161 tataaatcag cgttcttaaa caaatcctgc aaaaccgttg caactgttcg ccgggagaag
     2221 catttgcgcg tccgaaggcc tccccacaag cctttcacca tcccaggaac agggccgcac
     2281 cgggcaaccg gcgcgtccga aaggtacttc catgaacgct tccacagtcg cccgcgccgg
     2341 cggcctgacg ctgaccgccg cccttctggc ctcggtcgcc accccgaccc tcgccggctc
     2401 cttctatctg caggaacaat ccgcgcgcgg cgccgggcgc gcctattcgg gtgaagccgc
     2461 cgacaccggc gtgtcctcga tgtggtggaa ccccgccgca atcgcccgct cgggccgcga
     2521 agtctacgtc ggcgcccacg gcctgctgct cgactcggac gtcgacaacg ctggttcgac
     2581 catcacctat ccgggcggaa cgaccgttcc cgttcaaggc cagaaccata atggcgaccc
     2641 gatcgaaagc ggcatcgttc ccaacttcgc catctcgacc ccgatcggcg accgtttcgc
     2701 tgtcggcctc gccgtcgagg cgccttacaa cttcaccacc aagtaccagc ccggctcgtt
     2761 cgcccgctac gacgccctga cctcggagct gcgctcggcc aacatcagcc tcgtcggcgc
     2821 gatgaaggtc accgactggc tcgacatcgg cgcgggcttc gacgcccaat acgtcgaggc
     2881 caagctgacc tcggccctgc cgaacctgtc gccgctgctg cccgacggct cttcctcgct
     2941 ggaaggtgac ggctgggact acggctggaa cgtcggcgcc caggcccaca ccggtccctg
     3001 gcagttcggc ctgagctacc gctcggaaat cgaacatgag ctggaaggcg atgtcgccat
     3061 cagcggcctg ctgggtccga tcgcagccgg caacttctcc ggcaacggca ccgccagctt
     3121 caacaccccg tggttcgcct cggcttcggt gcgttacgcc gtcaacgaca agctgaccct
     3181 gaacgcccag gtcaaccgta tcggctggtc cgagttcgac gccattcgcg tgcgctacac
     3241 gaccggcggc gacaccttca tccaggacta caaggatgtg accaccggcg ccgtcggctt
     3301 cgactacgtc gccaacgagc gcgtcacctt ccgtggcggc gtcgcctatg atccgacccc
     3361 gacgcaggac ggcgagcgca cggcccgtat cccggacgcc gaccgctggt tgttctcggg
     3421 cggtgtttcc gtcaaggcca ccgacacgat gacggtcgac accggcctga cctacatcgc
     3481 gttctccgac agcgacatcg ccagcacgca cacttcgtat gcgggcacgg ctgctgccac
     3541 gacgtcgaac ctgcgcggta cggccgaagg ctacggcatc ggcgcttcgg tcggcgtgcg
     3601 ctgggcgttc tgatccaacc ataacggcga acaacggaac ggccggtcct cacaggaccg
     3661 gccgttttcg tttgcgcact cttccagcca gctcttcgtg acgtgtagcc agacaacaaa
     3721 agacccgccc ggtttgcacc gggcgggtct ctttaggtca ggtgacgcta aaccatatca
     3781 ggccttggcg acgtagccct tgccgccgtt ggacgggcga cgtgcgcgag ggcgacgctg
     3841 gatttcacca tcgggcttgg tcacgccggc cggacgcgca gcggcgttag cagccgaacg
     3901 agcgcggtcg gcagccaatg gatcgaacgg ctgggtcgag gccatgggac ggtccccacg
     3961 gtcggagtgg tgcgtgcgat cgcgttgacc gccaccatca cgaccaccac caccaccggc
     4021 gccagcgccg tcacgcggct tgcggacgcg gtgcggttgg ccgccgtgct cggacttgac
     4081 gccgtcacga cgcgggttgc gtggaccgcg gtcctgacga tcgccacggc cgtcttcagg
     4141 catggtcgcc ttcgagccga cgcccgaagc catgatcgac tgatcgagca ggccaagcga
     4201 cttgtcattg cgacggtcgg tggcggggat tttctggcgc gtgacctttt cgatgtcgcg
     4261 cagcagcttc atctcgtcac cggccacgaa gctgatggcc gagccgtcag cgccggcgcg
     4321 agccgtacga ccgatgcggt ggacataggc ttccggcacg aacggcagtt cgaagttcac
     4381 cacgtgcgag acgccgtcga cgtcgatgcc gcgcgcggcg atgtcggtgg cgaccagaac
     4441 gcgcagcttg ccggccttga aggcggccag ggtgcgctca cgttgcggct gggacttgtt
     4501 gccgtggatc gcgccggcct gaacgccgcc ggcttccaga taggcggcga ccttgtcggc
     4561 gccgtgcttg gtcttcgtga acaccaggca gcgcgtgtag ttgtcgtcgg cgaacatttc
     4621 ggtcagcagg gcgcgcttgc ggccttgctc gatgtggatg accgactggt tgatgcgctc
     4681 gaccgtggtc gattgcgggg tcacctggac cttgaccgga tccttgagca gttcaccggc
     4741 cagcttgccg atttcgctcg gcatggtggc ggagaagaac aggttctggc gcttggcggg
     4801 gatgcggctg acgatttggc ggatcggctt gacgaagccg aggtccagca tctggtcggc
     4861 ctcgtcgaga acgaaaatct cggtcgagga caggtcgatg gtcttctgct gcatatggtc
     4921 cagcaggcgg ccgggggtgg cgaccaggac gtcgaggccg gcctggatgg cgcgctcttg
     4981 cgggccgtat ttcacgccgc cgaacacgac agcgaccttg aagccaaggt gacggccgta
     5041 ggtcttgaag ctttcggcga tctggctggc cagttcgcgg gtaggggaca ggatcagggt
     5101 gcgggtggtg cgcggcgccg gggcgacccg gttttcggcc agacgatgca ggatcggcag
     5161 ggcgaaggcg gccgtcttgc cggtgccggt ctgggcgata cccagcaggt ccttgccgag
     5221 catgacgtca gggatggcct gcgcctggat cggggtcggg ttggtatagc cttcggacgc
     5281 aagcgcctga aggagagcct tgttcaggcc aaaggattcg aacgtggtgg tcaaagtcgt
     5341 ttctttcgcg tgcggcgacg cgcatcagcc atggctcata gccgaaaggc cgcgcaccgc
     5401 cagtcccgag tgagcttctg aatgaagcct gcggggtgga tcgggcgccg ccccgcgtgt
     5461 ccgggcagcg taatgaactt gaggatcggc cgctgctaca gtcaggtctt cattgggagg
     5521 aagacccaca cgcgctggag cgcaccgggc atcgcaattg tgcgacgcag cgcaaatggg
     5581 cgcttcatgt ggcgaagtca aggcgtaacg ctggtcagct gcccaacagg ctgacggcga
     5641 agttcacgcc cagcgcgacg atcaccgtgt tgaagacgaa ggcggtgacg ctgtggaccg
     5701 aggagattcg gcgcaggcgg cggtcggaga tctggacgtc ggccgtctgg caggcgacgc
     5761 cgatgatcaa tgcaaagtgg atgaagtccc aatagtctgg ctcggtctcg cccgggaaga
     5821 ccagaccacc ccggtccttg cccttgtcat ccgcgccata aaacccgtga gcatagtgct
     5881 gggcgaagat caggtggatg aagcaccagg acaaggtcac ggtggtcagc
//
LOCUS       DASVTM010000381         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_517331,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000381 DASVTM010000000
VERSION     DASVTM010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517331"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VF633_10410"
     CDS             complement(<1..140)
                     /locus_tag="VF633_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX8471515.1"
                     /translation="MPSFSRPLEDTLQRALVYANERRHEYATLEHLLLALIDDTDATA
                     VMT"
     gene            complement(152..484)
                     /gene="clpS"
                     /locus_tag="VF633_10415"
     CDS             complement(152..484)
                     /gene="clpS"
                     /locus_tag="VF633_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX8471516.1"
                     /translation="MPSRRPGDQEGGGFGATTITETKPKLQKPSLYRVLILNDDYTPM
                     EFVVYVLERFFQKSREDATRIMLHVHQHGVGVCGVFTYEVAETKVAQVVETARRHQHP
                     LQCTMEKD"
     gene            complement(844..1314)
                     /gene="phaP"
                     /locus_tag="VF633_10420"
     CDS             complement(844..1314)
                     /gene="phaP"
                     /locus_tag="VF633_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640421.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEX8471517.1"
                     /translation="MADTAETIKNTVDQTASTAKFQAEKVQAAGAQAFSEGVNKTTAA
                     MGELGAQSKQNLEALTASATAAQKGAEALSAQALSYGKTSWETSVAAAQSMSKARSVQ
                     ELIELQTSFTKSAMEVYLAEMTRMTETMTNSVKDSLKPINERVTASVEKFQATR"
     gene            1670..>2791
                     /locus_tag="VF633_10425"
     CDS             1670..>2791
                     /locus_tag="VF633_10425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX8471518.1"
                     /translation="MTALFRRIAAGVMAVLTAAAVSISPISPWSSSVEAQSSDNARYA
                     AIVVDAQTGEVLFARRADSPRYPASVTKMMTLYLTFEALEAGRVKLDDIVTVSPLAAS
                     QPPSKLGLASGQTIRFDDAMRATTVRSANDMAMLLAEHVGGSQARFAAMSTLKARQLG
                     MTQTNYANPNGLPDSRQVTSARDLAILARAIMRDYPQYYSYFGLHDWNFRGREYRNTN
                     GLLLGGNGYDGIKTGFTNGSGYNLAASAVRNQRRIIVIVMGGRSAPSRNNHVAELMNT
                     GFEVENRRAQGENIQVAQTFFEQRGFGVGDPDSTPGGGAPIAYASLNGERGSPVRSGD
                     DEDGESSSAVAYTALPPRPAPAPVSSPVHVAGPDYAPPAT"
BASE COUNT          479 a          894 c          860 g          558 t
ORIGIN      
        1 gtcatcaccg ccgtggcgtc ggtgtcatcg atcagcgcaa gcaacaggtg ctcgagcgtg
       61 gcgtattcat gccggcgctc gttggcgtaa accagggcac gttgcagcgt gtcttcgaga
      121 ggacgtgaaa atgagggcat gggagatcct ctcagtcttt ttccatagtg cattgtagcg
      181 gatgctggtg acggcgcgcc gtctcgacca cctgggcgac cttggtttcg gccacttcgt
      241 aggtgaagac gccgcagaca ccgacgccat gctggtgcac atgcagcatg atgcgggtcg
      301 cgtcctcgcg gctcttctgg aagaaccgct ccagcacata gacgacgaat tccatgggcg
      361 tgtagtcgtc gttcaggatc agcacccgat acaacgaggg cttttggagc ttgggctttg
      421 tttcggtgat cgtggtggcg ccgaagcctc caccttcttg atcacctggc cttcgtgacg
      481 gcattcgcat tccggagttt cggggagtcg aaacgctaga taggtagtgt tcgacggatt
      541 ttaagagacg tccagtcacg ggtttcgtga ttggtcttcc gacaacgcat ctctccccct
      601 ctttcgtcat cctccggcaa gcggcgcagc cgcgcagacc gggggaccca gcggcgccga
      661 aggcgacctg ttcagcgaga tgtcgaaggt cgtacgcgac agtttcgcgc tgccgcgcgc
      721 cgctgggtcc cccggtctcg ctgcgctcgc cggaggatga cgaaagaaaa caagacgaga
      781 ggagaaacaa aagggcccgg agctttcgct ccgggccctc ggtacgctta ctgaaaccgt
      841 cgcttagcgc gtggcctgga acttctcgac cgaggcggtg acgcgctcgt tgatgggctt
      901 gaggctgtcc ttgaccgagt tggtcatggt ctcggtcatc cgggtcattt cggccagata
      961 gacctccatg gcggacttgg tgaagctggt ctgcagctcg atcagttcct gaaccgaacg
     1021 ggccttggac atcgactggg cggcggcgac gctggtttcc cagctggtct tgccatagga
     1081 cagggcttga gccgacaggg cctcggcgcc cttttgggcg gcggtggcgg aggcggtcag
     1141 ggcctcgagg ttctgcttcg attgtgcgcc cagctcgccc atggcggcgg tcgtcttgtt
     1201 cacgccttcg gagaacgcct gagcgcctgc ggcttgaacc ttctcggcct ggaacttggc
     1261 ggtcgaggcg gtttgatcga cggtgttttt gatagtttcg gctgtgtcgg ccatgtgaac
     1321 tctcctgaac cctgtgatcg ggccccgtta cgtggcggac ccgcgtgatg atttcccgca
     1381 cctgctgaag ccggtgggat gagcgctata tggtgcaagt gcgaacagcg gtcaatattt
     1441 attgtgcact gcaccatatg aacaccgata accgtacaaa gtgcagaacc ggtgacgctt
     1501 gatggctgtt caattttccg ttgagacctc gttaaccacc gcgaaacccg cgacttgcat
     1561 gctagtcagg tcccacgtcg cgccagttga cgtaaagttg ggggctgcca acatccgcgt
     1621 cgcgaccctg gccacaggga cgtcacagtt acgaggctat cggttgccga tgaccgctct
     1681 attccgtcgc atcgccgctg gcgtcatggc cgttctgacg gcggccgccg tatcgatttc
     1741 gccgatctcg ccctggtcgt catcggtcga ggcgcaatcc tcggacaacg ctcgatacgc
     1801 cgcgatcgtc gtcgacgccc agaccggcga ggtcctgttc gcacgccgtg cagacagtcc
     1861 gcgctatccg gcgtcggtca ccaagatgat gaccctgtac ctgacgttcg aggcgctcga
     1921 ggccgggcgc gtcaagctgg acgacatcgt caccgtttct cccctcgccg cttcccagcc
     1981 gccttccaag ctcggtctcg cctcgggtca gaccattcgc ttcgacgacg ccatgcgcgc
     2041 gacgaccgtg cgctcggcca acgacatggc catgctgctg gccgagcatg tcggcggatc
     2101 ccaggcccgg ttcgcggcca tgtcgaccct gaaagcccgc cagttgggca tgacccagac
     2161 caactatgcc aatccgaacg gcctgcccga cagccgtcag gtcacctcgg cccgcgacct
     2221 cgccattctg gcccgcgcca tcatgcgcga ctatccgcag tactattcgt atttcggcct
     2281 gcacgattgg aacttccggg gtcgcgaata tcgcaacacc aacggcctgc tgctgggcgg
     2341 caacggctat gacggcatca agaccggctt caccaacggc tcgggctaca acctcgccgc
     2401 ctcggccgtg cgaaatcagc gccggatcat cgtcatcgtc atgggcggcc gctcggctcc
     2461 cagccgcaac aaccacgtcg ccgaactgat gaacaccggc ttcgaggtcg agaaccgtcg
     2521 cgcgcagggc gagaacatcc aggtcgccca gaccttcttc gagcagcgcg gcttcggcgt
     2581 cggcgacccg gactcgacgc cgggcggcgg cgctcctatc gcctacgcct cattgaatgg
     2641 tgagcgcgga agcccggtcc gtagcggcga cgacgaggat ggcgaatcct ccagcgcagt
     2701 cgcctatacg gccctgccac cacgtcccgc ccccgcgccg gtgtcttcgc ccgtccatgt
     2761 tgctggaccg gattatgccc cgccggcgac c
//
LOCUS       DASVTM010000382         5870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_783666,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000382 DASVTM010000000
VERSION     DASVTM010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783666"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..2009
                     /locus_tag="VF633_10430"
     CDS             162..2009
                     /locus_tag="VF633_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8471519.1"
                     /translation="MTDLAPTHTPADVQGRPGAGALLAEQMGEAGSRREKRKDVRPLG
                     RLLPYLVKHKLDAAFAFFWLLLSTAASLGLTATARGAIDQGFAGQGAHINRWFLLLGA
                     NALFLGAATAIRYFYVTKTGERVIADMRTALFGRILTLDPSFYAKMRTGEVLSRLTTD
                     IALVETLLTTSVSFALRNFLTLLGGIGMLFIVSPKLTGLVLLIAPVLLGPVFLFGRQV
                     RKLTVRSQDQFANAVGFAGESVDAIETVQAFGRVNSAIARFGAAVESAFGVSLIRMKA
                     RAFMTAIVIVVMFGGVTLVLWLGAQDVIRGAMSPGALLQFVLLSVFVAGAVGALGESW
                     GDVQKAAGAMERIDELIRAEASIAAPTVPIALPSPPRGEVSMSAVDFAYPGRPDLPAL
                     KGFSLTVRPGETVALVGPSGAGKSTVFRLLLRFYDPQSGLVSVDGVDVRAADPTDVRA
                     RFAWVSQETPLFSGSALENIRFGREDATEEEARTVAGEAQALTFIDALPEGFATPLGE
                     RGKSLSGGQRQRIAIARALVRDAPILLLDEATSALDAESERLVQIALDQAMESRTTIV
                     IAHRLATVLRADRIVVMEDGRVAEEGTHTELVAKGGLYARLAELQFRSE"
     gene            2071..2442
                     /locus_tag="VF633_10435"
     CDS             2071..2442
                     /locus_tag="VF633_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471520.1"
                     /translation="MLATTMFSLMLLAQTHSTHPVAPPGEADLAPAFGNTLLSTYPDG
                     RTARMWLQPGGRFEGRGRRGGLSSGTWRVRDGQVCFSQRRPIPLPGSFCTRIVPGGLG
                     TRWTAKAMTGETIRVELIAGR"
     gene            2487..3356
                     /locus_tag="VF633_10440"
     CDS             2487..3356
                     /locus_tag="VF633_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX8471521.1"
                     /translation="MIQPHALTGLSTVASDYDVLLCDIWGVIHNGRESWPDACDALVR
                     FNSAGGTVVLISNSPRPAPGVVEQLDALGVSRDSWKAIVTSGDATRAELTKRRPGPAW
                     IIGPDRDEPLYEGLGLERAEGAEDAAFLSVTGLIDDEAETPEDYRERLTPAAARDLEM
                     ICANPDRVVQRGDKIIYCGGSLADLYESMGGRVTMAGKPYGPIYALALAEAEQHLGRP
                     VDRSRVLCIGDGVVTDVMGANEQALDCLFIAQGIHGDAARGDDGKLDSARAADLLKAE
                     ATYARYAALDLVW"
     gene            3411..3863
                     /locus_tag="VF633_10445"
     CDS             3411..3863
                     /locus_tag="VF633_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8471522.1"
                     /translation="MVQQHPSGGYILDELHVGMTAEKAVTVTEARIMQFAEASDDFNP
                     VHMDEAFASKTAYRGRIAHGLLSASFGSAVVGTILPGAGAIYLTQTLAFHKPVRIGDV
                     VIARITVAAVDEVSARVTLRCEGYVNDEPIMDGEATVRVPRRRRPAKG"
     gene            3872..4825
                     /locus_tag="VF633_10450"
     CDS             3872..4825
                     /locus_tag="VF633_10450"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX8471523.1"
                     /translation="MAIEVIRGWRDLPETAKGAATAIGAFDGVHRGHQAVIADAREVA
                     DRIGAPLAVVSFDPHPRRWFQPDAAPFRLMTSAQMAQALEPLGVMGLYLLPFDAEMAA
                     MSDAEFARRVLADGLGIRHAAVGFDFTFGRGRTGSPEALRGYGETLGFTVSATDRIDD
                     PHGLKLSSSAVREALKAGDMDRAAAILSRPFAIAGEVIHGEKRGRTIGVPTANVALGD
                     YMRPAYGVYATRTRLPDGRVIDGVANLGVRPMFEIETPLLEVWLFDFSGDLYGQTVET
                     ELVARLRGELNFDGLGALKVQIDADAEAAKAILHQSGSAAF"
     gene            complement(4860..5414)
                     /locus_tag="VF633_10455"
     CDS             complement(4860..5414)
                     /locus_tag="VF633_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEX8471524.1"
                     /translation="MRIQAILIASAVALLAACSGEDKVKTATPEAPAAAALPPGRPAI
                     YAAAAEGPEPFVRALYAVFVAGGPKDTPPAPGQDPIYSRMLNATIGADVRVAKGEVPT
                     LNYDPICGCQDQGVFTLDSLAITQAGPRAADAAVVFTNAGETTRQTLKLVMEGPNWRV
                     DDVVDAEGKSLAKTMLEVITAAGG"
     gene            5637..>5870
                     /locus_tag="VF633_10460"
     CDS             5637..>5870
                     /locus_tag="VF633_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471525.1"
                     /translation="MSDVTDTSADSAVPTARDYRETVFLPETPFPMRGGLPQKEPAII
                     EQWGDLYATLRTERQKQNAPLYVLHDGPPYANGD"
BASE COUNT          935 a         1887 c         1978 g         1070 t
ORIGIN      
        1 agtggtagtt ggggtgcgta tccgctttca tggtcgtccg ggcctctggc gcgatgacga
       61 tgccgaccgt cggtgcgcat aaaatcaaaa atcccgcctg ctttaggcgg gaatgagcgg
      121 cggcgtttac accggagccc gttccggggc aagaggcggc tatgaccgac ctcgcgccaa
      181 cccacacgcc cgcagacgtc caggggcggc ccggggccgg cgcattgctg gccgagcaga
      241 tgggggaggc cggatcgcgc cgcgagaagc gcaaggacgt caggccgctc ggacgtctgc
      301 tgccctatct ggtcaagcac aagctagatg ctgcgttcgc cttcttctgg ctgctgctgt
      361 cgacggctgc gtcattgggc ctgaccgcga cggcccgtgg tgcgatcgat cagggctttg
      421 ccgggcaggg agcgcacatc aatcgctggt tcctgctgct gggcgccaac gcccttttcc
      481 tcggagcggc gacggcgata cgctattttt acgtcaccaa gaccggtgag cgggtgatcg
      541 ccgacatgcg tacggcgctg ttcggccgca tcctgacgct ggatccgtcg ttctacgcaa
      601 agatgcgcac gggcgaggtg ctgagtcgcc tgacgaccga catcgcgctg gtcgaaacct
      661 tgctgacgac gtcggtttcg ttcgcactga ggaatttcct cacactgctg ggcgggatcg
      721 gcatgctgtt catcgtcagc cccaagctga cgggactggt gcttttgatc gcgccggtgc
      781 tgctggggcc ggtgttcctg ttcggacggc aagtcaggaa gctgaccgtc cgatcccaag
      841 accaattcgc caacgccgtc ggctttgcgg gcgagagcgt tgacgccatt gagaccgtgc
      901 aggcattcgg acgggtaaac agcgccatcg cccggttcgg agcggcggta gaatctgcgt
      961 tcggagtctc gctcatccgc atgaaggcgc gcgccttcat gaccgcgatc gtcatcgtgg
     1021 tgatgttcgg cggtgtcacg ctggttctgt ggcttggggc gcaggacgtg atccggggcg
     1081 caatgtcgcc cggggcgttg ctgcagttcg tgctgctgtc cgtgttcgtc gccggcgccg
     1141 tcggggcgct cggcgaaagc tggggcgacg tccagaaggc ggcaggcgcc atggaacgga
     1201 tcgacgaact gatacgggcg gaggcgagca ttgccgctcc caccgtgccg atcgccttac
     1261 cgtcgccgcc gcgcggcgag gtgtcgatgt cggccgtgga cttcgcctat ccgggcaggc
     1321 ccgacctgcc cgcgctgaag ggcttctccc tgaccgttcg tccgggagag accgtggctc
     1381 tggtgggccc ctcgggcgca ggcaagtcaa ccgtgtttcg cctgttgctg cgcttctacg
     1441 atccgcagtc cggtctggtg tcggtggatg gtgtggacgt gcgcgcggcc gatccgaccg
     1501 acgttcgcgc ccgattcgca tgggtgtcgc aggagactcc gctgttctcc ggctctgcac
     1561 tggagaacat tcgcttcggg cgcgaagacg cgaccgaaga agaagcgcgg acggtggcgg
     1621 gggaggctca ggctctgacc ttcatcgacg cactgcccga agggttcgcg acgccgttgg
     1681 gcgaacgcgg caagagcctg tccggggggc agcgccagcg gatcgccata gcccgtgccc
     1741 tggtccgcga tgcgccgatc cttttgttgg atgaggcaac cagcgcgctc gacgccgaga
     1801 gcgagcgtct ggtgcagatc gccctcgatc aggcgatgga aagccggacg accatcgtca
     1861 tcgcccaccg tctggcgact gtgcttcgcg ccgaccgtat cgtcgtcatg gaagacggtc
     1921 gcgttgcgga agagggcacc cataccgaac tggtggccaa gggcggcctc tatgcccggc
     1981 tggcggagtt gcagtttcgc tcggagtgat tggatcggtt gaaatcgtcc gcaatcggag
     2041 cgattatcgc ttctattgag ggactattgt atgcttgcga cgacgatgtt cagcctgatg
     2101 cttctggcgc agacccattc tacacacccg gtggctccgc ccggcgaggc tgatctggcg
     2161 ccggcgttcg gcaatacgtt gctgtccacc tatcctgatg gccgcacggc tcggatgtgg
     2221 ctccagcccg gcggccggtt cgaggggcgg ggacgacgcg gcggcctgag cagcggaaca
     2281 tggcgggtgc gagacggcca ggtctgtttc agtcagaggc ggcccattcc cttgccgggg
     2341 tcattctgca ctcggatcgt gcccggcggt ctcggcacac gttggaccgc caaggccatg
     2401 accggtgaga ccatccgcgt cgaactgatc gccggacgat agacttcccg tcttcacccg
     2461 tcgcgcctcg cgcggtaagg ggcggcatga tccagcctca cgcacttacc ggcctgtcca
     2521 ccgtcgcctc tgattatgat gtcctgctgt gcgacatctg gggggtgata cacaacggtc
     2581 gggaaagctg gccggacgcc tgtgatgcgc tggtccggtt caacagcgcg ggcggtacgg
     2641 tcgtcctgat ctccaactcc ccgcgtcccg cgccgggcgt ggtagaacaa ttggatgcgc
     2701 tgggcgtgtc gcgcgatagc tggaaggcca ttgtcacgtc tggcgacgcg acacgcgcgg
     2761 agctcaccaa gcgcaggccg gggcctgcct ggatcatcgg ccccgaccgc gacgaaccat
     2821 tgtatgaagg tcttgggctc gaacgggccg agggcgctga ggatgcggcc ttcctgtcgg
     2881 tgaccggcct gatcgatgac gaggccgaga cacctgagga ttatcgtgag cgcctgacgc
     2941 ctgcggcggc gcgcgacctc gagatgatct gcgccaatcc cgaccgggtg gtccagcgcg
     3001 gcgacaagat catctactgc ggcggctcgc tggccgacct gtatgagtca atgggcggtc
     3061 gcgtgaccat ggcgggcaag ccctacggtc cgatctacgc cctcgctctg gccgaggcgg
     3121 aacagcatct gggccgtcct gtcgatcgct cgcgggtgct gtgcatcggc gacggcgtgg
     3181 tcaccgacgt catgggcgcc aacgagcagg cgctcgattg cctgttcatc gcgcaaggga
     3241 tccacggcga cgctgctcgg ggcgacgatg gcaagctgga ctctgcaaga gcggccgatc
     3301 tgctcaaggc tgaggccacc tatgcgcgtt atgcggcgct tgatctggtc tggtaaaggt
     3361 tcggcctcgc caaatcgcca caccggcgct aacaccctcc gatggtcgaa ctggttcagc
     3421 agcatccttc ggggggctat atcctcgatg aacttcatgt gggcatgacg gccgaaaagg
     3481 ccgtcaccgt caccgaagcc cgcatcatgc agttcgccga agcttcggac gacttcaatc
     3541 ccgtgcacat ggacgaggcg ttcgcctcca agaccgccta tcgcgggcgg atcgcacacg
     3601 gtctgctgtc cgcgtctttc ggctccgcag tggtcggcac catcctgccg ggcgccgggg
     3661 cgatctatct gacgcagacc ctggccttcc ataagccggt gcggatcggc gacgtggtca
     3721 tcgcccgcat cacagtcgcc gccgtggacg aggtctcggc ccgcgtgacc ctgcgctgcg
     3781 aaggctacgt caacgacgaa ccgatcatgg acggcgaggc caccgtccgg gtgccgcgtc
     3841 ggcgtcgccc cgctaagggc tgattccacc catggccatc gaggtcattc gaggctggcg
     3901 cgacttgcct gaaacggcaa agggcgcggc gaccgccata ggcgccttcg acggcgtcca
     3961 tcgcgggcat caggcagtga tcgccgatgc ccgcgaggtc gctgaccgga tcggcgcacc
     4021 tctggccgtc gtcagcttcg acccccatcc ccgacgctgg ttccagccgg acgccgcacc
     4081 cttccggctg atgacgtccg ctcagatggc gcaggcgctc gagccgctgg gcgtgatggg
     4141 gttgtacctg ctgcccttcg atgccgagat ggcggcgatg agcgacgctg agttcgcacg
     4201 gcgcgtgctg gccgacggtc tgggcattcg tcatgcggca gtcggcttcg acttcacttt
     4261 cggacggggg cggaccggat cgcccgaggc cttgaggggc tatggcgaga cgctcggctt
     4321 cactgtctcg gccaccgacc ggatcgacga tccgcatggg ctgaagctgt catccagcgc
     4381 ggtgcgtgag gcgctcaagg ccggcgacat ggaccgtgcg gcagcgatcc tgagccgccc
     4441 tttcgccatc gccggcgagg tcatccacgg cgaaaaacgc ggccgcacca tcggcgtgcc
     4501 gacggccaac gtcgcgctgg gagactatat gcgcccggct tacggggtct atgcgacgcg
     4561 cacgcggctg ccggatgggc gcgtcatcga cggcgtcgcc aacctcggcg tccgcccgat
     4621 gttcgagatc gaaaccccgc tgctcgaggt ttggctgttc gacttttccg gcgatctcta
     4681 tggccagaca gtcgaaacgg aactggtagc ccgcctgcgc ggcgagctga atttcgacgg
     4741 ccttggcgcc ctcaaggtcc agatcgatgc agacgccgag gcggcgaagg cgatacttca
     4801 tcagtcgggt tcggccgcct tttagcgctg ccgatcagtc gtggcggcaa gcgtgccgtc
     4861 tagccgcccg cagcggtgat gacttccagc atggtcttgg cgagcgattt gccttcggcg
     4921 tcgacgacgt cgtcgacccg ccagttcggg ccttccatga ccagtttcag cgtctggcgg
     4981 gtggtttcac ctgcattggt gaagacgacg gcggcgtcgg cggccctcgg cccagcctgt
     5041 gtgattgcca gcgaatccag cgtgaaaacg ccctgatcct ggcagccgca gatcgggtcg
     5101 tagttcagcg tcgggacttc gcccttggcc acccggacgt cggcgccgat ggtggcgttc
     5161 agcatgcgcg aatagatcgg gtcctgaccc ggtgcgggcg gcgtatcctt ggggccgcct
     5221 gcgacaaaga cggcataaag ggcgcgcacg aacggctccg gaccttcagc ggcggcggcg
     5281 tagatggcgg ggcgtcccgg cggtaaggca gcggcggcgg gcgcttcggg cgtcgcggtc
     5341 ttcaccttgt cctcaccgga acaggctgcg agcagggcga ccgcgctggc gatcaggatg
     5401 gcttggatac gcatgaaatc ccccaaagct tacagggccg ccagatcgac gctcttgaac
     5461 agcacgctac gcttttccca tccgctgtca cctgttacac cgcccatcat ggacgtccgc
     5521 ctgaacattc cggcccggat tagccgcccg gcgtagcgcc gacggggata tgtccccacg
     5581 cgctcgtcgg gtctgaagct accgataccg tcctcttgcc tgaaccgaga cgcaccatgt
     5641 ccgatgtgac cgatacttct gcagactccg ccgttcccac cgcccgcgac tatcgcgaga
     5701 ccgtcttcct gccggagacg ccgttcccca tgcggggcgg tctgccacag aaagagcctg
     5761 cgatcatcga gcagtggggc gacctctatg cgaccctgcg caccgagcgt cagaagcaga
     5821 acgcgccgct gtatgtcctg catgacggcc cgccctatgc caacggcgac
//
LOCUS       DASVTM010000383        14036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_622483,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000383 DASVTM010000000
VERSION     DASVTM010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622483"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VF633_10465"
     CDS             complement(<1..159)
                     /locus_tag="VF633_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8471526.1"
                     /translation="MSDSQRFPVAAHTLAYLAHKGAFDVARAAPSAVLAASVPTNPVV
                     VRRVTALLA"
     gene            337..2301
                     /locus_tag="VF633_10470"
     CDS             337..2301
                     /locus_tag="VF633_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX8471527.1"
                     /translation="MAGDAPRDDCAPPLSSPATADGAPAPVADKHSSHAKAGFWGLTI
                     GAIGVVFGDIGTSPLYALREAIAHAQSGVGGDLAVVGVVSLAFWALMIVVTFKYVIFL
                     MRADNKGEGGTLALMALAQHAMGRRSAWVFILGVCGAALFYGDGIITPAISVLSAIEG
                     VKDAPGVGTSLDAFIVPIAAAILVGLFMVQSRGTAGLAKFYGPITAVWFLSLGVLGLI
                     HIFDDVSILRALSPHYGVMLLLDDGFLGFIILGSVFLAVTGAEALYADMGHFGKAPIR
                     AGWLCFVLPCLTLNYLGQGAMILSHPGAADNPFWAMVPTAAYWPMLALATVATVIASQ
                     AVITGAFSVTQQAVQLGLLPRIDIRFTSETQAGQIFVPAVNTFLMVGVLTLLVVFQSS
                     HNLTAAYGVAVTGVMLINTLMSFPVITRKWGWPIWAVLACLIPFGFIDAVFLTSNLLK
                     IPDGAWLPLVLGAVLILIMWTWTRGSQILTAKAKKDSLPLTDLIEMLRARPPHRSPGT
                     AIFLTSDPDVAPVALMHNLKHNKVLHEKNIILTVRTTDRPRVPDSERVLMEPINDDFK
                     KITLSYGFMESPVVPRALGLCRKQGLKFDIMSTSFFLGRRSVIPSASQGMPLWQDKLY
                     IFLMRNAANPTDFFHIPPGRVVELGTQVSV"
     gene            2312..3706
                     /locus_tag="VF633_10475"
     CDS             2312..3706
                     /locus_tag="VF633_10475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX8471528.1"
                     /translation="MSGGTPKGKDSGARGRRLADKARGPRREPKPQVENPNDDSVDIG
                     VEARVAAGVLLNAALIRRGGLDEALALPTVTALPGPDRAFARAVAMAALRRLGEIDQI
                     LDRKLQKRPPEAVRTLMRISLAQTLVLETPAFAAVSTAVKLAERDPKTRPYKNLVNAV
                     LRGIEREGPGLTTAESNLPDWIAARWKQTYGEAALAGLALAAREEPATDLSLKPGQDA
                     AALAEAVEGEVLPGGSVRTGKRGDVASWPGFEDGSWWVQDAAAAVPVRLLDPKAGETA
                     LDMCAAPGGKTLQIAATGATVQALDRSDARLNRLRQNLARTGLEAAIAVMPAEEWLDS
                     RTFDAVLLDAPCTATGTFRRNPEALRATKPAEVAKLADVQHRLLDAAAERVGPGGRMV
                     YCVCSLEREEGETQIIAFLRRNPAFRTVKADPVAVGAPEEALTTEGWLRILPSMWAEK
                     GGLDGFFAAKLERV"
     gene            3754..4413
                     /gene="rpe"
                     /locus_tag="VF633_10480"
     CDS             3754..4413
                     /gene="rpe"
                     /locus_tag="VF633_10480"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX8471529.1"
                     /translation="MAAPLICPSILASDFAQLGAEVAAIEAAGADWVHVDVMDGHFVP
                     NITLGPDVVKALRPHTSLPFDVHLMVAPVDPWLEAYRNAGADILTVHPESGPHLHRTL
                     GRIRQLGAKAGVVLNPGTSLSVLEDVIDLVDLVLIMSVNPGFGGQSFIDASLRKIERT
                     RALLDAAGSPAHLQVDGGVTAANAGACVSAGADALVAGTAVFKGGPSAYAANIAALKS
                     T"
     gene            4410..6251
                     /locus_tag="VF633_10485"
     CDS             4410..6251
                     /locus_tag="VF633_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX8471530.1"
                     /translation="MNPLSPFAPRASEADGPAPGAIPGLKGAPVRTPVRSAGAHTGPT
                     LWPAIAGRLLTRQMWIELYGLPGYSLTLAGALSGGKATALAATPRDMRPADTAAGKAV
                     LDGRFTLAGVSMEVAAPEDPWDRPSPSRGFAVDLHAFAWMPGLMQQGDRGAREALRLT
                     LTWADHFTRWSPFAWDPGILARRVFNLSCAARRMGGVATEAERLRLCDLLGRQGRQLL
                     RPPGGLAGKAERLIAAALAGCVLAGPPGTSLRRAALSRLPAALRATVLGDGGHASRNP
                     EAGLALLLDLLTLDDALSQLSEPLPEAAGGAIQRLTSAMRVLTLPDGRLTSFQGGGPS
                     KADLVAAARAHDDWTQTPASTAPMGGAVGGLRRIHGALLTVVADTATLAHGAWGEAAC
                     AQPLALEICCGKDRLITGCGWTPRAADRQGLRLTPAHTTLTLGEQSLGDPLGGWKGEL
                     LGPRMIGRPIHVSCEHREAEGAVWLELEHDGWAVPFGLLHQRRLYLDQRLDELRAEER
                     LHPAPGHEEEVRAIAAPYAVRFHLEPGVQASLARDRRSILLRGHSGRGWWFRTDGPDV
                     AIEPSVHIDEGLTRRSLQIVVRGSARTDAETRIRWKLSPAGAAGDPV"
     gene            6254..6652
                     /locus_tag="VF633_10490"
     CDS             6254..6652
                     /locus_tag="VF633_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471531.1"
                     /translation="MARLIGQRWVFDEGNARIVVDNAFSWLGLAQERLVVNGETVQKA
                     RAVFSTRRDFLEPWLTLVGDEELRVALKSRILSISCTVTLGGRILTPVQHLTAQWTGI
                     HGSWPEEDQWKEAVGRKATYIAQDAVETKT"
     gene            6755..8359
                     /gene="purH"
                     /locus_tag="VF633_10495"
     CDS             6755..8359
                     /gene="purH"
                     /locus_tag="VF633_10495"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX8471532.1"
                     /translation="MPAAPDFPPAPDRVKPVRALISLSDKAGLEGAARAMHEVGVELI
                     STGGTRAAIAGFGLPVKDVADLTGFPEMMDGRVKTLHPVVHGGLLGVRDAADHKAAMD
                     EHGIGPIDIVWIDLYPFESTVASGAAFDAVIENIDIGGPAMIRSGSKNHGYVAVAVDA
                     ESIGLIVEALKADGATDLALRTRLAARALARTAAYDAAVSGWFAGQLEDVAPARKSIA
                     GSLAQTLRYGENPHQTGAFYRTGEARPGVAYAQQIQGKELGYNNIADADAAYELVAEF
                     EAPACVIVKHANPCGAAVGKDLSEAYARALECDAVSAFGGVIAVNRPLNGADARAITE
                     IFTEVVIAPGADEEAKAIFAAKKNLRLLITDGLPDPVGPGEVFRSVAGGFLVQSRDRS
                     RITAADLKIVTRRQPTPTEIQDMLFAFTIAKHVKSNAIVYAKDGQTAGIGAGQMNRRD
                     SARIAAIRAKEAGEAKGLAHSLAQGSACASEAFFPFADGLLEAVAAGATAVIQPGGSM
                     RDAEVIAAADEQGIAMAFTGVRVFRH"
     gene            complement(8508..9863)
                     /locus_tag="VF633_10500"
     CDS             complement(8508..9863)
                     /locus_tag="VF633_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8471533.1"
                     /translation="MAQFDPRRQPTRFAPHLLTAAAALSVAMLAGRVYQPAQAAEVPE
                     LTLQQVSALEARAFATAGAPAGLTAPEAVDVQIRKGETFEQAVRRTGIGEADAHAVAE
                     TISNAFDLADLRAGLKFETAISKPRGGLGDARLIGLTMRTGPASQLTVSRSFDGALRL
                     RALDEKVTHEIVVAQGAVNSSLASSAGAFGATAAIIRRATQVFAGKVDMRNDVRGADQ
                     FTLVFDRSVTEQGRTVSTGDLMYAEIKGQAFYRFQPAGARDAAYFDASGKNLRTTAMR
                     APLASFRRVSSGFGVRVHPLSGFRKMHQGMDYAATTGTPVVAPSDGVVVEARRWGGYG
                     NWLRIRHANGLETGYGHLSRYASGMRAGQRVRQGQIVAYVGSTGASTGPHLHYEIWRG
                     GQRINPAGIRTSEGTILAGADLTAFRAAKSRIDRIIAQGGESKPATMQAQVTGLRPAQ
                     G"
     gene            10206..10610
                     /locus_tag="VF633_10505"
     CDS             10206..10610
                     /locus_tag="VF633_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471534.1"
                     /translation="MLNVLLLSPSKKFRFLTLLRRVPNAARLRRLRVVEDAMASRVLR
                     LLSSFTLACGIAAVSTVGLAGFWKLIGGGALTIHGWIAMGLGILGTVGISWVLMGLAF
                     RSDREGWDDRVDNSLDPGRETHGESKDDDLIY"
     gene            complement(10611..11780)
                     /locus_tag="VF633_10510"
     CDS             complement(10611..11780)
                     /locus_tag="VF633_10510"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX8471535.1"
                     /translation="MAIRSDRTGTETGGVLEFFAGGGLAGIGLAYAGFHTLFANDIDP
                     AKMRSFRANHPHTPVHEGDIWDLTPGALPGVPDLCWASSPCQDVSLAGARGGLEARRS
                     GAFWGFWRLMQGLAAEGRAPQVVVIENVVGLLTSAKGRDFAAVCAALVEGGYRVGALE
                     MDAGLWLPQSRPRLFIVGLKDAENLEAGRLEAQTPGGAFHSPRLIAAHARLPEEVKAN
                     WAWWSVPAPPRRNLDLAALLEPDATVDWFDAPQTETLLALANPLHRARLEAALASGER
                     RVAAAFRRVRTENGAKIQRLELRLDGLAGCLRTPSGGSSRQYVLVCDGGTIRARRMTG
                     RETARLMGLDETYRLPSSENAALKLMGDAVAVPVVRALAQGLLAPALKMRSTIAA"
     gene            11890..12333
                     /locus_tag="VF633_10515"
     CDS             11890..12333
                     /locus_tag="VF633_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471536.1"
                     /translation="MRPLLLIVPLTLMASSCSQGTTEKTRSPAEAPTPVSLGGIDLNQ
                     PLGAVGTEPFWSVAITPDAMIFTGVDRDEQRASNPQPVVQGTTAIYTGATNDGAAMVV
                     TLIATECSDGMSDRVYPLTAKVELGEASLSGCAASQAFLDTRPPA"
     gene            complement(12330..12764)
                     /locus_tag="VF633_10520"
     CDS             complement(12330..12764)
                     /locus_tag="VF633_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471537.1"
                     /translation="MNNTISIETVVSQAGVAAGVEPDHAHTALVGALGLLEKHAEVEA
                     RDAFYAAVPGAAVLARSPEARPRGGGLMGGLMKSAGGVSGAALADAMGLLDRLKKVDV
                     GKAELKRLLPAARNAVTQATGRDWLGEAVRTIPGVGALLGDA"
     gene            12812..13972
                     /locus_tag="VF633_10525"
     CDS             12812..13972
                     /locus_tag="VF633_10525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8471538.1"
                     /translation="MSQTQNASPDHLIAPHLVQPNGGPTDLRALTALRFMAAMWVAVY
                     ALWDNLAVTFKPAVIAKGYLGVELFFVLSGFILCHVYLAAAGDKRFSYRDFLWARIAR
                     VYPLHIATLLAVMALGLAAIFAGMSIDSNVMAWSSLPANIAMVHAWGLAPEAGWNHRS
                     WSISAEWFAYLAFPAFAFVAWKVRHRPVAAVVGALILLGVVYFGFERLAGSPLTEATI
                     KWGALRIVPCFAYGCALYLAFRRAPLAIPGVIASIAAALMIAAAALQLWDGIIVAFAG
                     ALILGLASLPNARAGWLASKPAVYLGEISYSVYMVLVPWQLLAVNLAVKLTNAPDKHL
                     QLFVWLGILAALPVVAAASYHLVEFPARRALKGFATRHSGAAKPSPNAQLPA"
BASE COUNT         2203 a         4833 c         4579 g         2421 t
ORIGIN      
        1 ggccaacagc gccgtgaccc ggcgcaccac gacaggattg gtcggcaccg acgccgccag
       61 cacggcgctg ggcgccgcgc gcgcgacgtc gaacgcgccc ttgtgagcca gataggccag
      121 ggtgtgggcg gcgacgggaa agcgttggct gtcggacatg gtgcgggttt ccagccttta
      181 aggtagtgcg gcgtgaccgg attcacaaat ccagcctcgc cgggcaaaat ccggtcagca
      241 accaattacg agcggcacga ttgaaccttc ctcggaatga gcctacaggc gctccgcttt
      301 gatgggcgcg cggcatgcgc ggcccggagg atattcatgg ctggggatgc tccccgcgac
      361 gactgcgctc ccccgctttc gtcgcccgcg acggccgatg gcgcgcccgc gccggtcgcc
      421 gacaaacaca gctcacacgc caaggccgga ttctggggcc tgaccatcgg cgccatcggc
      481 gtcgtgttcg gcgacatcgg caccagccct ctctacgccc tgcgcgaagc catcgcccat
      541 gcccagtccg gtgtcggcgg cgatctggcg gtcgttggcg tcgtctcgct ggccttctgg
      601 gccctgatga tcgtcgtgac gttcaagtac gtcattttcc tgatgcgcgc cgacaacaag
      661 ggcgagggcg gaacgcttgc cctgatggct ctggcccagc acgccatggg ccgacgcagc
      721 gcctgggtct tcatcctcgg tgtctgtggc gcggcgctgt tctacggcga cggcatcatc
      781 acgccagcca tctcggtcct gtcggcgatc gagggcgtga aagacgcacc gggagtcggg
      841 acctcgctcg acgccttcat cgtaccgatc gcagccgcta ttctggtcgg cctgttcatg
      901 gtgcagtcgc gcggcacggc gggtctggcc aaattctacg gcccaatcac cgctgtctgg
      961 ttcctcagcc ttggtgtgct gggcctgatc cacatcttcg acgacgtctc gatcctgcgc
     1021 gccctgtcgc cccactacgg cgtgatgctg ctgctcgacg acggcttcct cggcttcatc
     1081 atcctcggct cggtttttct ggccgtgacg ggcgccgagg ccctctatgc cgacatgggc
     1141 catttcggta aggctccgat ccgcgccggc tggctgtgct tcgtcctgcc ctgcctgacc
     1201 ctcaactatc tgggtcaggg ggcgatgatc ctgtcgcatc cgggcgcggc ggataacccg
     1261 ttctgggcca tggtgccgac tgcggcctac tggccgatgc tggctctggc cacggtcgcc
     1321 acggtcatcg cctcgcaggc ggtcatcacc ggcgccttct cggtcacgca acaggcggtc
     1381 cagctgggtc tgctgccacg catcgacatc cgctttactt cggagaccca ggccggccag
     1441 atcttcgtgc ccgccgtgaa caccttcctg atggtcgggg ttctgacact tctggtcgtg
     1501 ttccagagct cgcataacct gactgcggcc tatggcgtcg ccgtcaccgg ggtgatgctg
     1561 atcaacacct tgatgagctt ccccgtgatc acgcggaaat ggggctggcc gatctgggct
     1621 gtactcgcct gtctgattcc gttcggtttc atcgacgccg tcttcctgac ctctaacctg
     1681 ctcaagatcc ccgacggcgc ctggctgccg ctggtgcttg gcgccgtgct gatcctcatc
     1741 atgtggacct ggacgcgggg ctcgcagatc ctgacggcca aggccaagaa ggacagcctg
     1801 ccgctgaccg acctgatcga gatgctgcgg gcccgcccgc cgcaccgttc gcccggcacc
     1861 gccatcttcc tgaccagcga tccggacgtt gcgccggtcg ccttgatgca caatctcaag
     1921 cacaacaagg tgctgcacga gaagaacatc atcctgaccg tgcgcaccac cgaccggccc
     1981 cgcgtgccgg actcggagcg cgtgctgatg gagcccatca acgacgactt caagaagatc
     2041 acgctgagct acggcttcat ggaaagcccg gtcgttccgc gcgccctggg cctgtgccgc
     2101 aagcagggtc tgaagttcga catcatgtcg accagcttct tcctcggccg gcgctcggtc
     2161 atcccgtcgg ccagtcaggg catgcccttg tggcaggaca agctgtacat cttcctgatg
     2221 cgcaacgcgg ccaaccccac cgacttcttc cacattccgc ccggacgcgt ggtggaactc
     2281 ggcacacagg tttcagtatg atggtttcgg tttgagcggc ggcacgccca agggcaagga
     2341 ttccggtgcg cgcggtcgtc gcctcgccga caaggcgcgc ggcccccgtc gcgagcccaa
     2401 gccgcaggtc gagaacccca acgacgactc ggtcgacatc ggcgtcgagg cgcgcgtcgc
     2461 agccggcgtc ctgctcaacg ccgccctgat ccggcgcggc ggtctcgacg aggcgctggc
     2521 gcttccgacc gtgacggcct tgcccggccc cgaccgcgcc ttcgcccgcg ccgtcgccat
     2581 ggcggccctg cgccgtctgg gcgagatcga ccagatcctc gaccgcaagc tgcagaagcg
     2641 cccgcccgag gccgtccgca cccttatgcg catttcgctg gcccagacgc tggtgctgga
     2701 aaccccggcg ttcgcagcgg tttccacggc ggtcaagctg gccgagcggg atcccaagac
     2761 gcggccctac aagaacctcg tcaacgccgt cctgcgcggc atcgagcgtg aaggtcccgg
     2821 cctgaccacg gccgagagca acctgcccga ctggatcgcc gcccgctgga aacagacata
     2881 cggcgaggcc gctctggccg gtctggccct cgcagcccgt gaagagcccg caaccgacct
     2941 gagcctcaag cccggtcagg acgccgcagc gttggccgaa gccgtcgagg gtgaagtcct
     3001 tcccggcggt tcggtccgta ccggcaagcg cggcgacgtg gccagctggc ctggcttcga
     3061 ggacggctcg tggtgggtgc aggacgccgc cgctgcggtc cccgtccgcc tgctggaccc
     3121 caaggccggt gaaaccgcgc tcgacatgtg cgccgccccc ggcggcaaga cgctgcagat
     3181 cgccgcgacc ggcgccaccg ttcaggcgct cgaccgctcc gacgcccgct tgaaccgcct
     3241 gcgccagaac cttgcccgca caggtttgga agccgccatc gccgtcatgc cagccgaaga
     3301 gtggctcgac agccgcacct tcgacgccgt cctgctcgac gcgccgtgca ccgcgaccgg
     3361 caccttccgc cgcaaccccg aagccctgcg cgccaccaag cccgccgagg tcgccaagct
     3421 ggccgacgtc cagcaccggc ttctcgacgc cgcggccgaa cgtgtcgggc cgggcggtcg
     3481 catggtctac tgcgtctgct cgctggaacg cgaagagggc gagacccaga tcatcgcctt
     3541 ccttcgccgc aaccccgcct tccgcaccgt gaaagccgac ccggtcgccg tcggcgcgcc
     3601 agaagaagcc ctgacgaccg aaggctggct ccgcatcctg ccctccatgt gggcggaaaa
     3661 gggcggcctc gacggcttct tcgccgcgaa gctggaacgg gtctgaacct cgggagcctt
     3721 gctccccgac gcgacccgcc ttatcccgtg cggatggccg cccctctgat ctgtccgtcg
     3781 atcctcgcca gcgatttcgc gcaacttggt gcggaggtcg ccgccatcga ggcggcgggc
     3841 gccgactggg tccatgtcga cgtcatggac gggcatttcg tgcctaacat cactctcggg
     3901 cccgatgtgg tgaaggcgct gcggccgcat acctccctgc cgttcgacgt gcatttgatg
     3961 gtcgcgccgg tcgatccgtg gcttgaagcc tatcgtaacg ccggggccga tatcctgact
     4021 gtccacccgg aaagcggacc gcacctgcac cgcaccctcg ggcgcatccg ccagctgggc
     4081 gccaaggccg gggtggtgct taatccaggg acatcgctga gcgtgctcga ggacgtcatc
     4141 gatctggtcg atctggtgtt gatcatgtcg gtcaatccag gctttggcgg ccagtccttc
     4201 attgacgcat ccctgagaaa gatcgagcgc acccgagcgt tgctcgacgc tgcggggtcc
     4261 cccgcccacc tgcaggtcga tggcggggtg acagcggcga acgccggcgc ctgcgtctct
     4321 gctggcgctg acgcgttggt cgcgggcacc gcggtgttca agggcggacc gagcgcctat
     4381 gccgctaata tcgcggcgtt gaaatcaacg tgaatcccct cagccccttt gccccccggg
     4441 ccagcgaggc cgacggcccc gcaccgggcg ccattcccgg tctgaagggc gcgcctgtcc
     4501 gtacgccggt tcgttccgcc ggcgcccata ccggtccaac cctgtggccc gcgatcgccg
     4561 gtcgcttgct gacgcgccag atgtggatcg agctctacgg tctgcccggt tacagcctca
     4621 cgctcgccgg cgcgctgtcc ggcggcaagg ccacggctct cgccgcgaca ccacgtgaca
     4681 tgcgtcccgc cgatactgcg gctggcaagg cggtcctgga cggccgtttt accttggcgg
     4741 gcgtcagcat ggaggtcgca gcacccgagg acccgtggga tcgccccagc ccctcgcgcg
     4801 gctttgctgt cgacctgcac gccttcgcct ggatgcccgg tctgatgcag cagggcgacc
     4861 ggggcgcgcg cgaagccctg cgtttgaccc tgacgtgggc cgatcatttc acccgctggt
     4921 cgccctttgc ctgggacccc gggattctgg cgcggcgggt gttcaacctg tcgtgcgccg
     4981 cccgccgcat gggcggcgtc gccaccgagg ccgagcggct gagactttgc gatcttctgg
     5041 gccgacaggg ccgacaactg ttgcgccctc ccggcgggct ggcgggcaag gccgagcgtc
     5101 tgatcgccgc tgcactggcc ggttgcgtgc tggcgggtcc tcccggcacg tctcttcgca
     5161 gggcagcatt gtctcgtctt ccggctgcgc ttcgggccac ggttctgggt gacggcggcc
     5221 acgcctcgcg aaatccagag gcggggctgg ccctgctgct cgacctgctg acgttggatg
     5281 acgccctctc ccagctcagc gaacccctgc ctgaggcggc gggcggcgcg atccagcggc
     5341 tgaccagcgc gatgcgcgtc ctgacactgc ccgacggtcg cctgaccagc tttcagggcg
     5401 gaggaccttc caaggctgat ctggtcgcag cggctcgcgc ccatgacgac tggacccaga
     5461 ctcccgcttc gacggcgccg atgggcgggg ccgtgggggg gctgcgccgc attcacgggg
     5521 cgctgctcac cgtcgtcgct gacacggcca ctttggccca cggggcttgg ggcgaggctg
     5581 cctgcgccca gccgttggcg ctggaaatct gctgcggcaa ggatcggctg atcaccggct
     5641 gtggctggac gccccgcgcc gcagatcgtc agggcctgcg cctgaccccg gcccacacca
     5701 ccctgacatt gggcgaacag tcgctcggcg atccgctcgg gggctggaag ggcgagttgc
     5761 tcggaccgcg catgatcggc cgcccgatcc atgtctcgtg cgaacaccgc gaggccgaag
     5821 gcgccgtctg gctggagctg gagcatgacg gctgggcggt gcctttcggc ctgctacacc
     5881 agcgccgcct ttatcttgat cagcgtctgg acgaacttcg cgccgaggaa cggctacatc
     5941 ccgcgcctgg acacgaagaa gaagtgcggg ccatcgcggc gccgtatgcg gtgcgcttcc
     6001 atctcgagcc cggtgttcag gcctcactgg cccgcgaccg ccgctcaatc ctgctgcgcg
     6061 gtcactccgg gcgtggctgg tggttccgca ccgatggtcc cgacgtggct atcgagccga
     6121 gcgtccacat cgatgagggc ctgacccgcc gctcgctcca gatcgtcgtc cgcggctccg
     6181 cccgcaccga tgccgagacc cgcatccgct ggaagctcag cccagcgggc gccgcgggcg
     6241 atccggtcta ggggtggccc ggctgatcgg gcagcgctgg gtttttgatg aaggcaacgc
     6301 cagaattgtg gtggataatg cgttcagctg gctcggcctc gctcaggaac ggctggtcgt
     6361 gaatggtgag acggttcaga aggcgcgggc tgtgttcagc acccggcggg atttcctcga
     6421 accttggctg acccttgtgg gcgatgaaga gcttcgcgtg gcgctcaagt cgcggatcct
     6481 gagcatctcc tgcacagtga ccctcggcgg gcgtattctg acgccggtgc agcatctgac
     6541 tgcgcagtgg acgggcatcc acggttcttg gccggaagag gaccagtgga aagaggccgt
     6601 cggacgcaag gcgacctata tcgcgcaaga cgcagtcgag acgaaaacct aaggcgatcg
     6661 gcaccgctgt tcctttgcta ctgcatggga ggtgaacccc gtccttcccc gtggtagatg
     6721 cccgcgcatc gctcccccga cccggatcac tcccatgcct gcagctcccg atttcccgcc
     6781 cgcacccgac cgggtgaagc ccgttcgcgc gctgatctcg ctgtccgaca aggccgggct
     6841 cgaaggcgct gcacgggcaa tgcatgaggt aggtgtcgaa ctgatctcga ccggcggaac
     6901 gcgtgcggcg atcgcgggct tcggcctgcc ggtcaaggac gtcgccgatc tgaccggctt
     6961 ccccgagatg atggacggcc gggtcaagac cctgcacccc gtggtccacg gcggcctgct
     7021 gggcgtgcgt gacgccgccg accacaaggc ggcgatggat gaacacggca ttggtccgat
     7081 cgatatcgtc tggatcgacc tctatccgtt cgagtcaacc gtcgcctcgg gagccgcgtt
     7141 cgacgccgtg atcgagaaca tcgacatcgg cggcccggcc atgatccgtt cgggctccaa
     7201 gaatcacggc tacgtcgccg tggcggtgga cgccgagtcc atcggcctga tcgtcgaggc
     7261 gctaaaggct gacggcgcga ctgacctggc cctgcgcacg cgtctcgcgg cccgcgcctt
     7321 agcccgcacc gccgcctatg acgccgccgt gtccggctgg ttcgcgggtc agcttgaaga
     7381 tgtcgccccg gcccgcaaat ccatcgccgg atcgctggcc cagacgctgc gctacggcga
     7441 gaacccgcac cagaccggcg ccttctaccg caccggtgaa gcgcgcccgg gcgtcgccta
     7501 tgcccagcag attcagggca aggaactcgg ctacaacaac atcgccgacg ccgacgccgc
     7561 ttatgagctg gtggccgagt tcgaggcccc ggcctgcgtc atcgtcaaac acgccaaccc
     7621 ctgcggcgcc gccgtcggca aggatctgtc cgaggcctat gcccgagcgc tggaatgcga
     7681 cgccgtctcg gccttcgggg gcgtcatcgc cgtcaaccgt ccactgaacg gcgccgacgc
     7741 gcgcgccatc accgaaatct tcaccgaagt ggtcatcgcc ccgggcgccg acgaagaagc
     7801 gaaagccatc ttcgctgcca agaagaacct gcgcctgctg attaccgacg gcctgcccga
     7861 tccggtgggc cccggcgaag tcttccgctc ggttgctggc ggcttcctcg tccagtcgcg
     7921 cgaccgctcg cgcattaccg ccgccgacct gaagatcgtc acgcgccgcc agccgacgcc
     7981 gacggaaatc caggacatgc tgttcgcctt caccatcgcc aaacacgtca agtccaacgc
     8041 catcgtctat gccaaggacg gccagacagc gggcatcggc gccggccaga tgaaccgtcg
     8101 cgactcggcc cgcatcgccg ccatccgcgc caaggaagcc ggcgaggcga aaggtctggc
     8161 ccattcgctg gcccaagggt ccgcctgcgc ctcggaggcc ttcttcccgt tcgctgacgg
     8221 cttgctcgaa gcggttgcag cgggcgcgac tgcagtcatc cagcccgggg gttcgatgcg
     8281 agacgccgag gtcatcgcgg ccgcagacga gcaaggcatt gccatggcct tcaccggtgt
     8341 gcgggttttc aggcattagg cgtagagcgc taccgctcgc cgcgcggcgg ctcgttgctt
     8401 gagcgcgagc cgcatgcgtc cctgccgggt cagcgatcgc acacagcaaa acggcggctc
     8461 gatcgagccg ccgtttttcg ttctgaagaa ccgctgaagc ggagcgccta cccctgagcg
     8521 ggacgcaggc cggtgacctg ggcctgcatc gttgcgggct tgctttcgcc gccctgggcg
     8581 atgatgcggt cgatgcgcga cttggcggca cggaaggcgg tcaggtcggc gccggcgagg
     8641 atggtgcctt cggaggtgcg gatgccggcc ggattgatcc gctgaccgcc gcgccagatt
     8701 tcatagtgca ggtgcggacc ggtcgacgct ccggtcgagc cgacataggc cacgatctgg
     8761 ccctgacgaa cccgctgccc ggcgcgcatg ccggaggcgt agcgcgacag gtgcccatag
     8821 ccggtttcga ggccgttagc gtggcggatg cgcagccagt tgccgtaacc gccccagcga
     8881 cgagcctcca ccaccacgcc gtcggaggga gctacgaccg gcgtgcccgt cgtggcggca
     8941 tagtccatgc cctggtgcat cttgcggaag ccggacagcg ggtggacgcg aacaccgaag
     9001 cctgagctga cgcggcggaa gctggcgagc ggcgcgcgca tcgcagtggt gcgcaggttt
     9061 ttgccgctgg cgtcgaaata ggcggcgtcg cgggcgccgg caggctggaa gcgatagaag
     9121 gcctggccct tgatctcggc gtacatcaga tcgccggtcg agaccgtgcg gccctgttcg
     9181 gtgaccgaac ggtcgaacac gagggtgaat tgatcggcgc cgcgcacgtc attgcgcatg
     9241 tcgaccttgc cggcgaacac ctgggtggcg cggcggatga tggcggcggt ggccccgaag
     9301 gctccggccg aggaggcgag cgacgagttc accgcgcctt gtgcgacgac gatctcgtgg
     9361 gtgaccttct cgtcgagagc gcgcaggcgc aaggcgccgt caaagctgcg cgacacggtc
     9421 agctggctgg ccgggccggt gcgcatcgtc aggccgatca gccgggcgtc gccgaggccg
     9481 ccacgcggct tggagatcgc ggtctcgaac ttcaatccgg cgcgcaggtc tgccagatcg
     9541 aaggcgttgg aaatggtttc ggccacggcg tgggcatcgg cctcgccgat acccgtgcgg
     9601 cgcacagcct gttcgaaggt ctcgcccttg cggatctgaa cgtcgacggc ttcgggagcg
     9661 gtaagaccgg ccggggcgcc ggcggtggcg aaggcgcggg cttcgagggc cgagacctgt
     9721 tgcaaagtca gttcgggaac ttccgccgct tgagcgggtt gatagacacg accggccagc
     9781 atggccaccg acagcgcagc agcggcggtc agaagatgcg gcgcgaaacg cgtcggttgg
     9841 cggcgtgggt cgaactgagc catcggtccc cagaaaaaga cgacgccagt cggcgccaag
     9901 caacgggcag gcaaggatcg cggccgggat ctccggcagt cgcgagacaa gccctataac
     9961 ctgctggtgt cataatggga agcagcccgc tacaaacggt taatggcggc gaggctggcc
    10021 tgtgttcccg gtagagcggg gtttctcgtc ggtaactcga acagtgtata accaaagaca
    10081 tccagctgag ccgttccgca caactgaagg tccgtcctca acactgaaag tgccttcgat
    10141 gtcacagctc gtggcttcaa ggcaacgccc gtatgctgcc aacagatcgc tagaccattg
    10201 ccgcgatgct gaacgtcttg ctactcagcc cgtccaagaa gttccgtttc ctgacccttc
    10261 tgcggcgcgt gccaaacgcc gcgcgattgc gcaggctgcg cgtggtagaa gacgccatgg
    10321 ccagccgcgt tcttcgcctt ctgtcatctt tcaccctcgc ctgcgggatc gcggctgtca
    10381 gcaccgtggg actggccgga ttctggaagc tgatcggcgg cggcgccctg acgatccacg
    10441 gatggatcgc catgggcctc ggcatcctag gcacggtggg cattagctgg gtcctgatgg
    10501 gcctggcctt tcgctcggat cgggaaggct gggatgatcg cgtcgacaac agcctcgatc
    10561 cgggtcgtga aactcacggc gagagtaagg atgacgacct gatctactga tcaagctgcg
    10621 atggtcgatc tcattttcaa cgccggggcc agcagaccct gcgccagggc cctgacgacc
    10681 ggcacggcca cggcgtctcc catcagcttc agcgcggcgt tttcactgga cggcaggcga
    10741 taggtctcgt ccagccccat cagccgcgcc gtttcccgcc cggtcattcg tcgggcgcgg
    10801 atcgtgccgc cgtcacaaac cagaacatac tgacgcgacg aaccgccaga tggggtccgt
    10861 agacatcccg ccagcccatc aagccggagt tccagtctct ggatcttggc cccgttctcg
    10921 gtccgcaccc tgcggaaggc ggcggcgacc cggcgttcgc ccgaggccag cgccgcctcg
    10981 agcctagccc tgtgcagtgg attggcgagg gccagcaggg tctcggtctg aggcgcgtcg
    11041 aaccagtcga cggtggcatc cggctccagt agtgcggcaa ggtccaggtt gcggcgcggc
    11101 ggcgcgggta cggaccacca ggcccagttc gccttgacct cctcagggag gcgcgcgtgg
    11161 gcggcgatta gtcgaggcga gtgaaaggcg ccgcctgggg tttgcgcctc gagacgaccc
    11221 gcttcaagat tttcggcgtc ctttagtccg acgatgaaca ggcgcggtcg cgactgcggc
    11281 agccacaggc ctgcatccat ctccagcgct ccaacgcggt agccgccctc gaccagcgcc
    11341 gcgcagacgg cggcgaaatc ccggcccttg gccgaggtca gcaaaccgac cacgttctca
    11401 atgacgacga cctggggagc ccggccttca gcggcgagtc cctgcatcag ccgccagaag
    11461 ccccagaaag ctcccgaccg gcgcgcctca aggccgcccc gcgctccggc cagactgacg
    11521 tcctgacagg gtgacgacgc ccagcacagg tcgggcacgc ccggcagggc cccaggcgtc
    11581 aggtcccaga tgtcgccctc gtgaacgggt gtgtggggat ggttggcgcg gaacgaccgc
    11641 atcttggccg ggtcgatatc attggcgaac agggtgtgaa aaccggcgta agccaggcca
    11701 atcccggcca gaccgcctcc ggcgaagaat tccagcacgc cgcccgtctc cgttcccgtc
    11761 ctgtcgctgc gaatcgccat gccgggactc taggcgccgc cgcgagttat ggcgaccggc
    11821 ctggcctgtg cagccttgac ccccgcagtg gacgtggcga cgatgcgcat gtcttggaag
    11881 gaccgttcta tgcgacccct gctcctcatt gttcctctca cgctgatggc gtcgtcctgt
    11941 tcgcagggca cgaccgaaaa gacgaggtcc ccggcggagg ctccgacccc ggtaagcctc
    12001 ggcgggatag atctgaacca accgttgggc gcggtcggga ccgagccctt ctggagcgtg
    12061 gcgatcaccc cggatgccat gattttcacc ggcgtcgatc gcgacgaaca gcgggcgagc
    12121 aatcctcagc ctgtcgtcca gggcacgacc gccatttata ccggggccac caatgatggc
    12181 gcggccatgg tcgtgaccct gatcgcgacc gaatgttcgg acggcatgag cgaccgcgtc
    12241 tatcccctga cggcgaaggt cgaactgggc gaggccagcc tcagcggctg cgcggcgtcg
    12301 caggcctttc ttgacacccg gccgccagcc taggcgtcgc ccagcaaggc gccgacacca
    12361 gggatcgtgc gaaccgcctc gccgagccag tcgcgacccg tggcctgcgt cacagcgttg
    12421 cgagcggcgg gcagcagtcg tttgagttcg gccttgccga cgtcgacctt cttgagccgg
    12481 tccagcaggc ccatagcgtc agcgagagcc gcgccggata ctccgccagc actcttcatc
    12541 agcccgccca taagtccgcc gccgcgcggc cttgcctcgg gcgacctcgc gagaactgca
    12601 gcgcccggca cggcggcgta gaaggcgtcg cgcgcctcca cttcagcgtg cttttccagc
    12661 aaacccagcg cgcccaccag ggctgtatgc gcgtgatcag gctcaacgcc ggctgcgacg
    12721 ccggcctgcg acactacggt ttcaatagat atggtgttgt tcatggcgct ccttaacggt
    12781 tctcaaagcc caacgatcct agggtgcggc catgagccag acgcagaacg cctccccgga
    12841 tcacctcatc gcgccgcatc tcgtccagcc gaacggcggt cccacggatc ttcgcgctct
    12901 gacggcgctg cgcttcatgg ctgccatgtg ggtcgccgtc tacgctctct gggacaatct
    12961 ggcggtaacc ttcaagcccg cggtaatcgc caagggctat ctcggagttg agctgttctt
    13021 cgtgctgtcc ggattcatcc tgtgccacgt ctatctggcg gccgcggggg acaaacggtt
    13081 cagctatcgc gactttctct gggcccggat cgcacgggtt tatcccctgc atatcgccac
    13141 attgctggcc gtgatggcgc tcggccttgc agcgatcttc gccggcatgt cgatcgacag
    13201 caatgtgatg gcctggtcgt cgttgcccgc caacatcgcc atggtccatg cttggggcct
    13261 ggcgcccgag gccgggtgga atcaccgttc gtggtcgatc tcggctgaat ggttcgccta
    13321 tctcgcgttc cccgcattcg ccttcgtggc ctggaaggtg cgtcatcggc ctgtcgcggc
    13381 ggtggtcggc gcactcatcc tgctcggggt ggtgtatttt ggttttgaac gtctcgcggg
    13441 ctcccccttg accgaggcga ccatcaaatg gggcgcgctc cggatcgtgc cctgcttcgc
    13501 ctatggctgc gccctgtacc tcgcgttccg acgggcgccg ctcgccattc cgggcgtaat
    13561 cgcttcgatc gcagccgcat taatgatcgc tgccgccgct ttgcaactgt gggacggaat
    13621 catcgtggcg ttcgcaggcg cgctcatcct cggcctcgcc agcctgccga acgcacgggc
    13681 cggatggctg gcgtcaaagc ctgccgtgta tctcggcgag atcagctact cggtctatat
    13741 ggtattggtc ccgtggcagc ttttggccgt taacctcgcg gtcaagctga caaacgctcc
    13801 ggacaagcac cttcaacttt tcgtgtggtt ggggattttg gcggcgctgc cggtggtggc
    13861 ggccgcttcc taccatctcg tcgaattccc cgcccggcgg gccctcaagg ggttcgcgac
    13921 tcgccatagc ggcgcagcaa agccctctcc caacgcgcaa ctgcccgcct gaaagtgagg
    13981 gtttcccctc gcagactaat cgggtaggca atgggtgggc gtaacaatcg gacaca
//
LOCUS       DASVTM010000384        10874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_405958,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000384 DASVTM010000000
VERSION     DASVTM010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405958"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..245
                     /locus_tag="VF633_10530"
     CDS             42..245
                     /locus_tag="VF633_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471539.1"
                     /translation="MPIEPPRLSRRRLDVLRRLVRSAADRMSDDQILDLADTLHDALR
                     ERRRSRDRAQNPASSAQIYRLPN"
     gene            complement(242..529)
                     /locus_tag="VF633_10535"
     CDS             complement(242..529)
                     /locus_tag="VF633_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471540.1"
                     /translation="MSDTENESAPETAAAPAAVDPAAYEAVFAALCRELGYCLHLKGE
                     KRVIAALPLGLDATVKAVLEAEGADFLNTSGSLKRQIRDCLKANLPPRAEA"
     gene            complement(526..1365)
                     /gene="fghA"
                     /locus_tag="VF633_10540"
     CDS             complement(526..1365)
                     /gene="fghA"
                     /locus_tag="VF633_10540"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX8471541.1"
                     /translation="METTKTHIVHGGTLRYLKHDSVSTGTPMTLSVFVPGGEGPFPVL
                     IWLSGLTCTEDNFTTKAGAYRAAAEHGVIIVAPDTSPRGEGVADDEAYDLGQGAGFYV
                     DATEGPWAPHFRMETYVTEELIALIDAEFPTTRTRSISGHSMGGHGALTLALRHPELF
                     KSVSAFALIASPTRCAWGEKAFTAYLGEDRSVWDAHDTAKLVESGAAKGVYDDILIDQ
                     GDADSFLVDQLKPELLVDAANAVGQRLTLRMQPGYDHSYFFMASFIADHVAFHAQRLK
                     ATT"
     gene            complement(1371..1754)
                     /locus_tag="VF633_10545"
     CDS             complement(1371..1754)
                     /locus_tag="VF633_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8471542.1"
                     /translation="MFSHVMLGADDIEASRKFYDAILGALGVAPGVIDPKGRVWWRTE
                     RGNFAISKPIDGGPASCANGGTIGFAAPSVEMANAFHEAGLANGGTAIEDPPGTRESP
                     FGALHLAYLRDPSGNKVAVMHRLRG"
     gene            complement(1769..2185)
                     /locus_tag="VF633_10550"
     CDS             complement(1769..2185)
                     /locus_tag="VF633_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8471543.1"
                     /translation="MSVPRVLGLNHVTLAVSDLGRATAFYRDVLGCVLRAEWPEGVYL
                     EAGPVWLCLSVDPKVRTEPHPDYTHVAFDVDDATFEALEPLVRAQAKLWKENRSAGRS
                     LYFLDPDGHKLELHVGSLATRLKAYRDRAAPDMIFY"
     gene            complement(2182..2319)
                     /locus_tag="VF633_10555"
     CDS             complement(2182..2319)
                     /locus_tag="VF633_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471544.1"
                     /translation="MFSRITDGKIETVSRITHALPLEDIATAFIVHAREHPQDRGVLA
                     Q"
     gene            complement(2312..2761)
                     /locus_tag="VF633_10560"
     CDS             complement(2312..2761)
                     /locus_tag="VF633_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471545.1"
                     /translation="MIVWRPGWKTAPPDERAAAEAELIARPAWVIDGVSRRVRDAADV
                     VVFLDVDRRTCLMRCAGRNWRYLFRSRPGLPENCPEILIVPRLIGIIGNFPRLIRPSI
                     LSELEGRDGAFQVRTSHDRNAMLKALIAGRAEGRILGKKQQGEISHV"
     gene            complement(2860..3966)
                     /locus_tag="VF633_10565"
     CDS             complement(2860..3966)
                     /locus_tag="VF633_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX8471546.1"
                     /translation="MKTRAAVAFEAKQPLEIVEVDLEGPRFGEVLIEIKATGICHTDA
                     YTLDGLDSEGIFPSILGHEGAGVVVEVGPGVTSLAVGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRSTQGKGLMPDGTSRFSYKGQAIAHYMGCSTFSNFTVMPEIAVAKIRKDAP
                     FDKACYIGCGVTTGVGAVVNTAKVEPGANAVVFGLGGIGLNVIQGLKMVGADMIIGVD
                     LNPEKEAWGRRFGMTHFVNPKDTPDVVARLVELTDGGADYTFDCTGNTVVMRQALEAC
                     HRGWGESIVIGVAEAGKEISTRPFQLVTGRVWRGSAFGGARGRTDTPKIVDWYMDGKI
                     EIDPMITHTLPLEDINKAFDLMHAGESIRSVVVF"
     gene            complement(4155..7577)
                     /locus_tag="VF633_10570"
     CDS             complement(4155..7577)
                     /locus_tag="VF633_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8471547.1"
                     /translation="MKRIWLTTVAVAVLAVQAPVSAMAQTQSPPNPTPTPALQDAPPA
                     PTPEKPAPEPVLETATPADVATAAPATAPDATATPDKPKWDVNNPPGPSHDVKIDVTQ
                     GTWMSLDVSPDGRTLVFDMLGDIYVMPIGGGEARVIASGVAWEMQPKWSPDGTRIAFT
                     SDRGGGDNIWTMNADGSDAKQVSKETFRLVTQPEWTPDGQFIVGRKHFTSSRSLGAGE
                     MWMWHRSGGGGVQLTTRRTQQKDTGEPAFSPDGRYLYFSDDATPGGQFEYSKDVNGQI
                     YVIQRLDRQSGEVEEFLSGPGGSIRPTPSPDGKSLAFIRRVRYQSTLYVMDIESGRET
                     PIYAHLDRDLQETWAIHGVYPAMSWMPDGKSIVFWAAGIFHKIDVASQEVSDIPFHIA
                     DTRRVEPAVRTTVDVAPDTFAVKMIRWAHASPDGSKVVFESLGNLWIRDLPTGTARRL
                     TSQSDHFELYPSWSRDGRSIVYTTWSDKDLGTVRVIPASGGTGRVVTQNPGHYIEPSF
                     SPDGKTIAYRAVADGFLTTGLWGRNPGIYRVPTAGGDATLVTDDGTTPQWGATNDRIF
                     YLGRKDENSALKSVDLSGHEERTHLTSQWASDFEISPNEQWVAWTERFNAYVAPFAAT
                     GRPVEMGSKAEALPQTRVTRDAGMWLTWSGDSSKLSWAIGPEFFTRSLTDSFSFEEGA
                     PAELPKPAEQGINIGFTATADKPSGVLVLTGARIITMNGDQVIEDGVIVVNENRIEAI
                     GPRGSVTVPAGATVMDMAGKTITPGFVDAHWHGSMAADGIIPQQSRVDYASLAFGVTT
                     IHDPSNDSSEIFAHSEMARAGKVVGPRIFSTGTILYGAQTPFTAQIDSLEDALSNLRR
                     TQALGAISVKSYNQPRRDQRQQIIEAARELNINVVPEGGSLFQHNMSMVVDGHTTIEH
                     SIPLAHLYDDVRQLWGQTDVAYTPTLIVAYGGAFGENYWYQESPVWNDAILQQYVPRR
                     VLDARARRPTTQPAEEWNHIAIAQQAKALMDVGVNVQTGAHGQREGLGEHWEIWMMAQ
                     GGMTPLQALRTATLNGAKALGLDKDVGSLEVGKLADMVVMNTNPLENIRNTHDIAYTV
                     ANGRVYDSHMDEVGRRMRPRQPFWFASSNGEAGPAGVAVASAADHGGGDTGD"
     gene            7743..9041
                     /locus_tag="VF633_10575"
     CDS             7743..9041
                     /locus_tag="VF633_10575"
                     /inference="COORDINATES: protein motif:HMM:NF019404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HEX8471548.1"
                     /translation="MPDTITRTTSTSWFSRIGGAFTGVLIGFILIIACIGGLFWNEGR
                     AIKTEKGLTEGAAVVIEIQPGEVVAANDQKLVHVSGPAVVTAPLEDTDFGVTATGLHL
                     ARKVEMYQWKEASKSESRTRLGGGEETVTTYTYTKEWASSANDSSQFAEPNGHQNPEF
                     DIQAADILAQDATLGAFKLEDNVVGQVGGETALRLTPADLETVDAAVGDIGRYVSISQ
                     NRIMLTERRPTVVAPSATSDDEEAATDETVTDETNDDATNETTVPTQTVTPAASIGDV
                     RVSYDLTPAGPISVVARQNGEGFLPYYAKNGEEFLLVDNGDKPASDMFKGAQDANQIM
                     TWIIRVVGLVLLIAGFGLLMAPLGVLADVLPLAGTIVRMGTGLIAFALGMLIGTVTIA
                     LAWIVFRPLIGIAILVVGGGIAFSAYRLARGKGKKAEAAA"
     gene            9181..9777
                     /locus_tag="VF633_10580"
     CDS             9181..9777
                     /locus_tag="VF633_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471549.1"
                     /translation="MLKSLTALAVASSVLLAGCDSAEKTATPVAPAPVTAADTTAARA
                     AVRDLIAADGDCTPDRIQASDRAKTKDFGGVLGVVAGCSTGIIDVWSRLYLARPGAAP
                     VAAPLLVFDIRGDGEWHAESNVPNLVWSEEAGTLEASIRSQTTNCGWDARWRWDGTRM
                     ALVEMKTVGCDAEHSTDVVVWPTTPATPEPTPTPIPVD"
     gene            complement(9809..>10874)
                     /locus_tag="VF633_10585"
     CDS             complement(9809..>10874)
                     /locus_tag="VF633_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX8471550.1"
                     /translation="SIVLLKNEGGVLPLKAEGQKIALIGPFASDLDVFGPWTIWGDET
                     HRISLEAGFRNALKNDADLTVIKGCDIETAIDDGFAAAVLAAGQADVVVLALGEGSRF
                     TGEAQSRTEITIPDIQLNLAAAVAATGKPVVVLLRNGRALELSGAVKNAQGIVVTWFL
                     GSEMGNSVADVLFGDHSPSGRLPVSFPHKSGQQPFSYDHKRTGRPANPALVSEEYTAR
                     YRETTNTALYPFGHGLTYGAVTYAAPVVASPTMAWDGTLEVSVDVRNAGEVAAVETVQ
                     LYIGDVVASLTQPGQRLRDFRKVALAAGETETVRFKLTREQLEFIGAQGTPTVEPGAF
                     DVWLAPSAQVGTPVRFTLVR"
BASE COUNT         1847 a         3684 c         3425 g         1918 t
ORIGIN      
        1 ggccattacg atctgcgtca ggaccgcccg aggttaaatc gatgcctatc gaaccgcccc
       61 gcttaagccg gagaaggctg gacgtgttgc gccgtctggt gcgtagcgcg gccgaccgca
      121 tgtccgacga tcagattctc gacctcgccg acaccctgca cgacgccttg agagaacgtc
      181 gccgatcgcg ggatcgggcg caaaatccgg cgtcgtccgc gcagatctat cgcttgccga
      241 actaggcctc ggcgcgcgga ggcaggttgg ccttgaggca gtcgcggatc tgccgcttca
      301 gcgatcctga cgtattgagg aagtctgcgc cctcggcctc caacacggcc ttgaccgtgg
      361 cgtcgagccc gagcggcagc gccgcgatga cccgcttctc acccttcaga tgcaggcaat
      421 agcccagctc gcggcacagg gcggcgaaca ccgcctcata ggctgcaggg tcgaccgctg
      481 cgggagctgc tgcagtttca ggtgcgctct cgttttccgt atcgctcatg tcgtcgcctt
      541 caatcgttgt gcatggaagg ccacgtgatc cgcaatgaaa ctggccatga agaaatagga
      601 atggtcgtag ccgggctgca tccgcagcgt caggcgctga ccgacggcat tggccgcatc
      661 gaccagaagt tcaggcttta gctgatcaac gaggaagctg tctgcatcgc cctgatcgat
      721 cagaatgtcg tcatagacac ccttggccgc cccgctttcg accagcttcg ccgtgtcgtg
      781 agcgtcccag actgaccggt cttcgcccag ataagcggta aacgccttct cgccccatgc
      841 gcaccgggtc ggcgacgcga tcagcgcaaa ggccgagact gatttgaaca gttccggatg
      901 acgcaatgcc agcgtcaggg ccccatgccc gcccatcgaa tgtccgctga tcgagcgcgt
      961 cctcgtcgtc ggaaactccg cgtcaatcag cgcgatcagt tcctcggtga cgtaggtctc
     1021 catgcggaaa tgcggcgccc agggtccttc cgtcgcatcg acgtagaacc cggccccctg
     1081 ccccagatca taggcctcat cgtcggcgac accttcaccg cgcggggacg tatccggcgc
     1141 aacgatgatc accccatgct cggccgccgc ccgataggct cccgccttgg tggtgaaatt
     1201 gtcctcggtg caggtcaggc ccgacagcca gatcagcacc gggaacggcc cctcaccgcc
     1261 tggaacgaac actgacaggg tcatgggcgt gcctgttgag acgctgtcat gcttcagata
     1321 gcgcagcgtt ccgccgtgaa cgatgtgggt cttggtggtt tccatcaagt tcagccgcgc
     1381 agccggtgca tgaccgcaac cttgttcccg gacgggtcgc gcaaataggc gagatgaagc
     1441 gcgccgaacg ggctttcgcg cgtgccgggc ggatcctcga tggccgtgcc tccattggcc
     1501 agaccggcct catggaaagc gttggccatc tcgaccgacg gcgccgcgaa accaattgtg
     1561 ccgccgttgg cgcaggacgc cgggccgccg tcgatcggct tcgaaattgc gaaattgccc
     1621 cgttcggtac gccaccagac ccggcctttc gggtcgatga cgcccggcgc aacgccgagg
     1681 gcgcccagga tggcatcgta gaatttccgc gaggcctcga tgtcgtcggc ccccagcatg
     1741 acgtgactga acattgctgc gctctttcct agtagaagat catgtccggt gcggccctgt
     1801 cgcgatacgc tttcaggcgc gtcgccagag atccgacatg cagttccagc ttatggccgt
     1861 cggggtcgag gaaatataga gaccgtcccg cgctgcggtt ctctttccag agtttggcct
     1921 gcgcccgaac cagcggctcc agcgcttcga acgtcgcatc atcgacgtcg aaggcgacgt
     1981 gggtatagtc gggatgaggc tccgtccgca cctttgggtc caccgacagg cacagccaaa
     2041 ccggccctgc ctccagatag acgccttcag gccattcggc gcgaagcaca caacccagaa
     2101 cgtcacgata aaaggccgtc gcccgcccca aatcgctgac tgcgagggtc acgtggttca
     2161 gacctaaaac ccggggcacg ctcattgcgc cagaacacca cgatcctgcg gatgctctcg
     2221 cgcatgtacg atgaacgccg tggcgatgtc ttccagcggg agggcgtgcg tgatcctcga
     2281 aacggtctcg atcttgccgt ccgtgatgcg actaaacatg cgagatttct ccttgctgtt
     2341 ttttaccgag gattcgaccc tcggcgcgcc ctgcaatcaa ggccttcaac atcgcgttac
     2401 ggtcgtggga agtcctcacc tggaacgctc cgtcacgtcc ttcgagttca gacaggatgc
     2461 tgggccggat cagccgtgga aagttcccga tgatcccaat gagcctcggc acgatcagga
     2521 tttcgggaca gttctccggc agccccggtc ggcttcggaa caggtagcgc cagttccgtc
     2581 ctgcgcatcg catcaggcag gttctccggt ccacatccag aaaaaccacg acgtctgccg
     2641 cgtcccgcac ccgtcgggat accccgtcga tcacccacgc cggacgcgcg atcagttccg
     2701 cctcggcggc ggctcgctcg tccggcggcg cggtcttcca gccgggacgc cagacgatca
     2761 tatcaagccc gaagaccgga agatcgagcg ctcgcccgat ctcaccggcc agcgtggtct
     2821 tgccgcttcc ggcgttgccg gtgacgtgga tgcgtcgcgt cagaacacca cgacgctgcg
     2881 gatgctctcg ccggcatgca tcaggtcgaa cgccttgttg atgtcttcga gcggcagggt
     2941 gtgggtgatc atcggatcga tctcgatctt gccgtccatg taccagtcga cgatcttggg
     3001 cgtgtcggtg cggccgcgcg cgccgccaaa ggccgagccg cgccagacgc gaccggtgac
     3061 cagctggaac ggccgggtgg agatttcctt gccggcctcg gccacgccga tgacgatgct
     3121 ttcgccccag ccgcggtggc aggcctccag cgcctgacgc atgacgaccg tgttgccggt
     3181 gcagtcgaag gtgtagtccg cgccgccgtc ggtcaattcg accagacgcg cgacgacgtc
     3241 gggcgtgtcc ttgggattga cgaaatgggt catgccgaag cggcggcccc acgcctcctt
     3301 ctccgggttc aggtcgacgc cgatgatcat gtcggccccg accatcttca gcccctgaat
     3361 gacgttcagg ccgatgccgc ccagaccgaa gaccacggcg ttcgcgcccg gttcgacctt
     3421 ggccgtgttg accaccgcgc cgacgccggt ggtcacgccg caaccgatgt agcaggcctt
     3481 gtcgaacggc gcgtccttac ggattttcgc caccgcgatc tcgggcatga cggtgaagtt
     3541 cgagaaggtc gaacagccca tatagtgggc gatcgcctga cccttgtagc tgaagcggct
     3601 cgtgccgtcg ggcatcaggc ccttgccctg cgtcgagcgg atcgcggtgc acaggttggt
     3661 cttgcggctc aggcacgact tacactggcg gcattccggc gtgtacagcg ggatcacgtg
     3721 atctccgacg gccagcgatg tcacgcccgg cccgacctcg acgacgacgc cggcgccttc
     3781 atgacccagg atcgagggga agatgccctc tgagtccaga ccgtccagcg tataggcgtc
     3841 agtgtggcag atgcccgtcg ccttgatctc gatcagcacc tcgccgaagc gcggcccctc
     3901 cagatcgacc tcgacgatct cgagcggttg tttcgcttcg aaggcgacgg cggcgcgggt
     3961 cttcatgggc agggtctcca gaactcactt cccttctccc cttgcgggag aaggtggcgg
     4021 cctctggccg acggatgagg ggtcgcaccg tcggcgagcg gattcaaggc ctcgatggcc
     4081 aaacatcgcg tgacccctca tccgacccgc tacgcggccc accttctccc gcagggggag
     4141 aagggctcct ggcgtcagtc ccccgtgtcg ccgccgccat gatccgccgc tgaagctacc
     4201 gccacacccg ccggcccagc ctcaccgttg gagctcgcga accagaacgg ctgacgcgga
     4261 cgcatgcggc gtccgacctc atccatatgg ctgtcgtaca cccggccgtt ggcgaccgtg
     4321 taggcgatgt cgtgggtgtt gcggatgttc tccagcgggt tggtgttcat caccaccatg
     4381 tccgccagct tgccgacctc cagcgaaccg acatccttgt ccaggcccag cgccttggcg
     4441 ccgttcaggg tcgcggtccg cagcgcctga agcggcgtca tcccgccctg cgccatcatc
     4501 cagatttccc agtgctcgcc caggccttcg cgctggccgt gggcgccggt ctggacgttg
     4561 acgccgacat ccatcagcgc cttggcctgc tgggcgatag cgatgtggtt ccactcttcg
     4621 gccggctggg tcgtgggccg gcgcgcacgg gcgtccagca cccggcgcgg gacgtactgc
     4681 tgcaggatcg cgtcgttcca gaccgggctt tcctgatacc agtagttttc gccaaacgcc
     4741 cccccatagg ccacgatcag ggtcggggtg taggcgacat cggtctgacc ccacagctgg
     4801 cgcacgtcgt catacagatg ggccagcggg atcgagtgct cgatcgtcgt gtggccgtcg
     4861 accaccatcg acatattgtg ctggaacagc gagccgccct cggggacgac gttgatgttc
     4921 agttcgcgcg cggcctcgat gatctgctga cgctggtcgc gacgcggctg gttgtagctc
     4981 ttgacgctga tggcccccag cgcctgcgtc cgccgcaggt ttgacagggc gtcctcaagg
     5041 ctgtcgatct gggcggtgaa cggcgtctgc gccccgtaca ggatggtccc ggtcgagaag
     5101 atgcgcggcc ccacgacctt gcccgcccga gccatttcgg agtgggcgaa gatctcgctg
     5161 ctatcgttcg acggatcgtg gatcgtcgtc accccgaagg caagcgacgc atagtcgacc
     5221 cggctctgct gcgggatgat gccgtcagcc gccatcgagc cgtgccagtg cgcatcgacg
     5281 aagcccggcg tgatggtctt gccggccatg tccatgaccg tcgcgcccgc aggcacggtg
     5341 accgatccac gcggcccgat agcctcaatc cggttctcgt tgacgacgat gacgccgtcc
     5401 tcgatcacct gatctccgtt catggtgatg atccgcgcac cggtcaggac cagaacgccc
     5461 gacggcttgt cggcggtggc ggtgaagccg atattgatcc cctgctcggc cggcttgggc
     5521 agttccgcag gcgcgccctc ctcgaagctg aagctgtcgg tcaggctgcg ggtgaagaac
     5581 tccggcccga tggcccagct cagcttcgac gagtcgcccg accaggtcag ccacatcccc
     5641 gcgtcgcgcg tcacccgcgt ctgcggcagc gcctcggcct tggagcccat ctccaccgga
     5701 cggcccgtgg cggcgaacgg cgcgacatag gcgttgaacc gctcggtcca agcgacccac
     5761 tgctcgttcg gtgagatctc gaagtccgag gcccactgcg aggtcaggtg ggtgcgctct
     5821 tcatggccgc tcaggtcgac cgacttgagc gccgaattct cgtccttgcg accgaggtag
     5881 aagatccggt cgttcgtcgc gccccactgc ggcgtcgtcc cgtcgtcggt caccagcgtg
     5941 gcgtccccgc ccgcagtcgg cacgcggtag atgcccggat tacgccccca cagcccggtg
     6001 gtcaggaacc cgtcggcgac cgcgcgatag gcgatggtct tgccgtccgg cgagaagctc
     6061 ggctcgatat agtggccggg attttgcgtc acgacacgac ccgttccgcc cgacgccggg
     6121 ataacccgca ccgtgccgag gtccttgtcc gaccaggtcg tatagacgat cgaccggcca
     6181 tcgcgggacc atgacggata gagctcgaaa tggtcgctct ggctggtcag gcgacgcgcg
     6241 gtgcctgtcg gcaggtcgcg tatccacaga ttgccgagcg actcgaacac caccttcgac
     6301 ccgtccggcg aggcgtgggc ccagcggatc atcttcacgg cgaacgtatc cggggcgacg
     6361 tcgaccgtgg tgcgaaccgc aggctcgacc cggcgcgtgt cggcgatgtg gaacgggatg
     6421 tcggagactt cctgcgaggc cacgtcgatc ttgtggaaga tgccggccgc ccagaacacg
     6481 atcgacttgc cgtccggcat ccagctcatc gccggataga cgccgtggat cgcccaggtc
     6541 tcctgcaggt cgcggtccag atgggcgtag atcggcgtct cacggccgct ctcgatatcc
     6601 atcacataga gggtggactg gtagcgaacg cggcggatga aggccaacga cttgccgtcc
     6661 ggtgacggcg tcggacggat cgaaccgccc ggcccagaca ggaactcctc gacctcgccc
     6721 gactgacgat ccagccgctg gatcacatag atctggccgt tgacgtcctt ggagtattcg
     6781 aactgtccgc ccggcgtggc atcatccgaa aaatacagat agcggccatc aggcgagaag
     6841 gcgggctcgc ccgtatcctt ctgctgcgtc cggcgggtcg tcagttgcac gccgccgcca
     6901 ccggatcgat gccacatcca catttccccg gcgcccagcg agcggctgga ggtgaaatgc
     6961 ttgcgaccga cgatgaactg gccatcgggg gtccactcgg gttgcgtcac cagacggaac
     7021 gtctccttcg acacctgttt ggcgtcagag ccgtcggcgt tcatcgtcca gatattgtca
     7081 ccgccgcccc ggtccgaggt gaaggcgatg cgggtcccgt ccggcgacca tttcggctgc
     7141 atctcccacg cgacgcccga agcgatgacc ctcgcctcac cgccgccgat cggcatgacg
     7201 tagatatcgc ccagcatgtc gaacaccagc gtccggccgt ccgggctgac gtccagcgac
     7261 atccacgtgc cctgcgtcac gtcgatcttc acgtcatgcg acggccccgg aggattgttg
     7321 acgtcccatt tcggtttgtc gggcgtagcg gttgcatcgg gagcggtcgc gggcgccgcc
     7381 gttgcgacat cggccggcgt tgcggtctcc agcaccggct cgggcgcggg cttctcaggc
     7441 gtcggcgcag gcggcgcatc ctgaagtgcg ggagtgggtg tggggttcgg cggcgactgc
     7501 gtctgggcca tcgcgctcac aggcgcctgc accgccagga ccgccacggc caccgtcgtc
     7561 aaccagatcc gcttcatgtc ttccaaaccc cgtacaaaca ggccgtcatc tagcgacata
     7621 gcgcccggtt tggcgagagc gccttggcat ccaccaacct tcacctttgc gacagaaccg
     7681 cgtggtacag gcctcggcta cagcgcaccc cggggagcgc gtcctgttcc ggggggaata
     7741 tcatgcctga caccatcaca cgcacgacga gcacgtcgtg gttttcacgc atcggcgggg
     7801 ccttcaccgg cgtcctgatc ggcttcatcc tgatcatcgc ctgcatcggc ggcctgttct
     7861 ggaacgaggg ccgggccatc aagaccgaaa agggcctgac cgaaggcgcc gccgtcgtta
     7921 tcgaaatcca gccgggtgag gtcgtcgcgg ccaatgacca gaagctggtc cacgtctccg
     7981 gccccgccgt cgtcaccgcc cctctggaag acaccgattt cggcgtgacc gccaccggcc
     8041 tccatctcgc ccgcaaggtt gagatgtacc agtggaagga agcctccaag tccgagagcc
     8101 gcaccaggct gggcggcggc gaggagactg tcaccaccta tacctacacg aaggaatggg
     8161 cttcctcggc gaatgactcc agccagttcg ccgagcccaa cggccaccag aaccccgagt
     8221 tcgacattca ggcggcggac attctggccc aggacgccac cctcggcgcc ttcaaacttg
     8281 aagacaatgt cgtcggccag gtcggtggcg agaccgccct gcgcctgacg cctgcggacc
     8341 tcgaaacggt cgacgcggct gtcggcgata tcggtagata cgtctcgatc tcgcagaacc
     8401 gcatcatgct gacggagcgc cgcccgaccg tggtcgcccc ctccgcgacc agcgatgacg
     8461 aggaagccgc tacggacgaa acggtcaccg acgaaaccaa cgacgacgcc acgaacgaga
     8521 ccaccgtccc gacccagacg gtgacgcccg ccgccagcat cggcgatgtg cgcgtcagct
     8581 acgacctgac cccggccggt ccgatcagcg tcgtcgcccg tcagaacggc gaaggcttcc
     8641 tgccgtacta cgccaaaaac ggcgaagagt tcttgctggt cgacaacggc gacaagcctg
     8701 cgtcagacat gttcaagggc gcgcaggacg ccaaccagat catgacctgg atcatccgcg
     8761 tcgtcggtct ggtgttgctg atcgccggct tcggcctgct gatggcgccc ctcggcgtcc
     8821 ttgccgatgt cctgcctctg gcgggtacga tcgtgcgcat gggcacaggc ctgatcgcct
     8881 ttgctctggg catgctgatc ggcacagtga ccatcgccct ggcgtggatc gtcttccgtc
     8941 cgctgatcgg gatcgccatc ctcgtcgtcg gcggcggcat tgctttcagc gcctacaggc
     9001 tggcgcgcgg caaaggcaag aaggccgagg cggcagcctg aatcagcacc gtctcgccgc
     9061 tggctgcgcg gtccggatgc gtttcgctca tagcggctgc tgcagtgagc cattctctct
     9121 cttggagcag ccgccgtttg tgactacccc tgacgccaac ctgttcagga atggacccct
     9181 atgctgaaat ccctgaccgc cctcgccgtc gcttcaagcg tcctgctcgc gggttgcgat
     9241 tccgccgaga aaactgcgac gccggtcgcc ccggctccgg tgacggccgc cgacacaact
     9301 gcggctcgcg cggcggtacg cgacctgatc gcggccgacg gagactgcac gccggatcgt
     9361 atccaagcct cggatcgggc aaaaacaaag gatttcggcg gcgttcttgg ggtcgtggcg
     9421 ggatgttcga cgggcatcat cgacgtatgg agccgcctgt atctggcgcg cccgggcgcg
     9481 gctccggtcg ctgcgcccct tctggttttc gacattcgtg gtgacggcga gtggcatgcg
     9541 gaatcgaacg tgccgaacct cgtctggagc gaggaggccg gaaccctcga ggccagtatc
     9601 cgcagccaga ccaccaactg cggatgggac gcccgctggc gttgggacgg aacgcggatg
     9661 gctctggtcg agatgaagac ggtcggctgc gatgctgagc actctaccga tgtcgtcgtc
     9721 tggccgacga cgcccgcgac gcccgagccg acgccgacgc cgatccccgt cgactgacca
     9781 caggcctccc caaggggagg cctgtcgcct atcgaaccag cgtgaaccgc actggcgtcc
     9841 cgacctgagc cgacggcgcg agccacacgt cgaacgcgcc cggttcgacc gtcggcgtgc
     9901 cttgcgcgcc aatgaactcg agctgctcgc gggtcagctt gaagcgaacg gtttcggtct
     9961 cgccagccgc cagcgccacc ttgcggaagt cccgcaaacg ctggccgggc tgagtcaggc
    10021 tggcgaccac atcgccgata tagagctgca ccgtctcgac cgctgcgacc tcgccggcgt
    10081 ttctgacatc taccgagacc tccagcgtcc cgtcccaggc catcgtcggg ctggccacca
    10141 ccggagcggc gtaggtcacc gcgccatagg tcaggccgtg accgaacgga tagagcgccg
    10201 tgttcgtggt ctcgcgatag cgggcggtgt attcctcgct gaccaatgca gggtttgccg
    10261 ggcggccggt gcgcttgtga tcgtaggaga acggctgctg gcccgacttg tgcgggaagc
    10321 tgaccggcag gcggcccgac ggcgaatgat cgccgaagag cacgtcggcg acgctgttgc
    10381 ccatctcgga gccgaggaac cacgtcacca cgatcccttg ggcgttcttc acagcgccgg
    10441 acagttccag cgcacgaccg ttacgcagca ggaccacgac cggcttgcct gtggccgcca
    10501 ctgcggcggc gaggttgagc tggatatcgg ggatggtgat ctcggtgcgc gactgggcct
    10561 cgccggtgaa gcgcgagcct tcgcccagcg ccagcaccac gacgtcggcc tgaccggcgg
    10621 ccagaaccgc cgcagcaaag ccgtcgtcga tcgccgtctc gatgtcgcag cccttgatga
    10681 ccgtcaggtc cgcgtcattc ttgagcgcgt tgcggaagcc agcttccagc gaaatccggt
    10741 gcgtctcgtc gccccagatg gtccacggcc cgaacacgtc gaggtccgag gcgaacggtc
    10801 cgatgagcgc gatcttctgc ccctcggcct tcaacggcag gacgccgccc tcgttcttca
    10861 gcagcacgat cgac
//
LOCUS       DASVTM010000385         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_675165,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000385 DASVTM010000000
VERSION     DASVTM010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675165"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..483)
                     /locus_tag="VF633_10590"
     CDS             complement(142..483)
                     /locus_tag="VF633_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471551.1"
                     /translation="MNRFLSMKRLSLMFLGAFAVLVAGTLTFQVFWLNPGERCEKNGK
                     WYDVQSRTCATPIYIPDITGRPAGVSRAEASAAKNAELVDLEQQVAKEQAANAAAVQR
                     ARADLKARQGL"
     gene            609..1373
                     /locus_tag="VF633_10595"
     CDS             609..1373
                     /locus_tag="VF633_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX8471552.1"
                     /translation="MKVLVPVKRVIDYNVKARVKADQTGVDLANVKMSMNPFDEIAVE
                     EAVRLKEGKEHHAAGTVTEIVVVSIGVQQAQETIRTALAMGADRGILIQSDSDLEPLA
                     VAKLLKAVVDEEKPDLVLMGKQSIDGDNNAVGQMLAALLDWPQATFASKVEIADGKAT
                     VTREVDGGLQTLAATLPAVVTVDLRLNTPRYASLPNIMKAKKKEIAMKAVADYGVDTA
                     PRLTVVKVTAPATRSAGIKVADAAELVSKLKTAGAL"
     gene            1375..2304
                     /locus_tag="VF633_10600"
     CDS             1375..2304
                     /locus_tag="VF633_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX8471553.1"
                     /translation="MAVLVIADHDGSAVRDTTNKTVTAALKLGADVDVLVVGSGATAA
                     AGSAAKITGVRKVLLAESAGLGHQLAEDVEETVLALAEGYDAILSPATNDGKNFMPRL
                     AAKLDVAPISDIIEVVSADTFVRPIYAGNALETIQSSDAKKVITVRPTNFDAAANDGS
                     ASVESVGGKEAGMATFIGEELVKSDRPELGAAKIIVSGGRAMGSADEFHAIMEPLADK
                     LGAAIGASRAAVDAGYAPNDYQVGQTGKVVAPGLYIAIGISGAIQHLAGMKDSKIIVA
                     INKDPDAPIFQVADYGIVGDYKTIVPELMTALG"
     gene            2324..2611
                     /locus_tag="VF633_10605"
     CDS             2324..2611
                     /locus_tag="VF633_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX8471554.1"
                     /translation="MGVTARLAIKLTPGSAADRVDGWGVDTDGRPVLKVRVRARPVEG
                     EANEALMKLLAKTLGVSRSSVVLARGGQSRLKMIAIDGLDDEAVRARLSED"
     gene            2698..>3326
                     /locus_tag="VF633_10610"
     CDS             2698..>3326
                     /locus_tag="VF633_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8471555.1"
                     /translation="MKMFRMRGASLAVLACAMVGAFSLSACATTDSSTPSSDTAETKA
                     PKPLATGLDYAANSDPYPSTYQPLPRQNSAIVGATVFTGAGQRIENGVVLMADGKVQA
                     VGGADLAVPAGYTVIDGKGRFVTPGVIDVHSHLGVYPSPGVQGMSDGNEATSPNTAQV
                     WAEHSVWPQDPGFNTARAGGVTTLGILPGSANLFGGRGVTLRNVPALSV"
BASE COUNT          601 a         1066 c         1071 g          588 t
ORIGIN      
        1 cttcttgacc cgcaatgcct tttggagcgg cgtctcgcca aggatgtcgt cggtcgaggg
       61 cgcagtcggt tcgtcgttga tgttgtcgct cattccgtca tcctagcacg ccgacccaac
      121 gcttgcccta caaaccctga actacagacc ttggcgggcc ttgagatcgg cgcgggcccg
      181 ctgaacggcc gccgcgttgg cggcctgctc tttggccacc tgctgttcaa ggtccaccag
      241 ctcagcattt ttggccgcag aggcttcggc gcggctaacg ccagccggac gaccggtgat
      301 gtcgggaatg tagatggggg tggcgcaggt tcggctctgg acgtcgtacc atttgccgtt
      361 cttctcgcag cgctcacccg ggtttagcca aaagacttga aaggtgagtg tgccggcgac
      421 cagaacggcg aaggcgccca agaacataag gctcagacgt ttcatgctca ggaagcggtt
      481 catcgggtct ctctgcaagg gcttggagtg ctttgggtca tttagttcac gttgcgttac
      541 gttgacaggg gctccgctcc gtaccatctc ctgcgccaac aattcgacca agatatggat
      601 agctccccat gaaggtactc gtccccgtca aaagggtgat cgactataac gtcaaggccc
      661 gcgtcaaggc ggaccagacc ggcgtcgacc tcgccaacgt caagatgagc atgaacccgt
      721 tcgacgagat cgccgtcgaa gaggccgtgc gtctgaagga aggcaaggaa caccatgccg
      781 ccgggaccgt gaccgagatc gtggtcgtct ccatcggcgt gcagcaggcg caggaaacca
      841 tccgcaccgc gctcgccatg ggtgcggacc ggggcatcct gatccagtcg gactcagatc
      901 tggagccgct ggcggtcgcc aagctgttga aggcggtcgt cgacgaagaa aagccggacc
      961 tcgtcctgat gggcaagcaa tctatcgacg gcgacaataa cgcggtcggc cagatgctgg
     1021 cggcgctgct ggactggccg caagcgacgt tcgcctcgaa ggtggagatc gccgacggca
     1081 aggcgaccgt gacccgtgaa gtcgatggcg gcctgcagac gctcgctgcg accctgccgg
     1141 cggtggtgac ggtggacctg cgcctcaaca cgccgcgcta cgcctcgctg cctaacatca
     1201 tgaaggccaa gaagaaggag atcgcgatga aggcggtcgc cgactatggc gtcgataccg
     1261 cgccgcgcct gacggtggtc aaggtgactg cacctgcgac ccgctccgcc gggatcaagg
     1321 tggctgacgc cgccgaactg gtttccaagc tcaagaccgc aggagcgctg taagatggct
     1381 gttctcgtca ttgccgacca cgacggttcg gccgtccgcg acaccaccaa taagacggtg
     1441 actgcggctc tcaagctcgg cgccgatgtc gacgtgctgg tcgtcggctc cggagctacg
     1501 gctgctgccg gcagcgcagc caaaatcacc ggcgtccgta aggtgctgct ggccgaaagc
     1561 gccggtctgg gccaccagct ggccgaggac gtcgaggaga ccgtgctggc tctcgccgaa
     1621 ggctacgatg ccatcctctc gcccgcgacc aacgacggca agaatttcat gccgcgcctg
     1681 gccgccaagc tggacgtcgc gccgatctcg gacatcatcg aagtcgtctc ggctgatacg
     1741 ttcgtgcgtc cgatctacgc cggcaatgcg ctggagacga tccagtcctc cgacgccaag
     1801 aaggtcatca ccgttcgtcc gacgaatttc gacgccgccg ccaatgacgg ctcggcctcg
     1861 gtcgagagcg ttggcggcaa ggaagccggc atggcgacct tcatcggcga ggaactggtc
     1921 aagtcggacc gtcccgaact gggcgccgcc aagatcatcg tctcgggtgg tcgcgccatg
     1981 ggctcggccg acgagttcca cgccatcatg gagccgctgg ccgacaagct gggcgccgcc
     2041 attggcgcct ctcgtgcggc cgtcgacgcc ggctatgccc cgaacgacta tcaggtcggc
     2101 cagaccggca aggtcgtggc gccgggcctc tacattgcga tcggcatttc gggcgccatc
     2161 cagcacctcg ccgggatgaa ggactccaag atcatcgtcg ccatcaacaa ggatcccgac
     2221 gccccgatct tccaggtcgc ggactacggc atcgtcggcg actacaagac catcgtcccc
     2281 gagttgatga ccgctctggg ctgatgactg ttttgatggc gagatgggcg tgaccgctcg
     2341 tctcgccatc aaactgaccc ccggatccgc cgccgatcgg gtggatggat ggggtgtcga
     2401 tacggacgga cggcctgtcc tcaaggttcg tgtccgtgcc cgcccggtcg agggcgaggc
     2461 caatgaagct ctaatgaaat tgctggcgaa gacgctgggc gtgtcgcgat cttcggtcgt
     2521 cttggcgcgt ggcggtcagt cgcggctgaa gatgatcgcc atcgacggac tcgatgacga
     2581 ggcggttagg gcgcgattgt ccgaagactg attttcgacc tttacagggc gcgagacgca
     2641 ccgcttctat agccgcgttc agaagcccgg cggtggtccg gggcacgagg atacttcatg
     2701 aagatgtttc gcatgcgggg agccagcctc gccgtgctcg cctgcgccat ggtcggcgcg
     2761 ttcagtcttt cggcctgcgc cacgaccgat agttccaccc cgagctccga caccgccgag
     2821 accaaagctc ccaagcctct ggcgacaggg ctggactacg cagccaacag cgatccctat
     2881 ccgtcgacct accagcctct gccacgccag aactcggcga tcgttggggc gacggtcttc
     2941 accggcgccg gtcagcgcat cgagaacggc gtggtcctga tggccgacgg caaggttcag
     3001 gcggtcggcg gcgctgatct ggccgtaccg gcgggctata cggtcatcga cggcaagggt
     3061 cggttcgtga cccccggcgt gatcgacgtc cactcccacc tcggcgtcta tccttcgccg
     3121 ggcgtgcagg gcatgagcga cggcaacgaa gcaacgagcc ccaacaccgc gcaggtctgg
     3181 gccgagcatt cggtctggcc gcaggatccg ggcttcaaca ccgctcgcgc cggtggcgtc
     3241 accaccttgg gcatcctgcc gggttcggcc aacctgttcg gcggtcgcgg cgtgaccctg
     3301 cgcaacgtgc cggcgctgag cgtgca
//
LOCUS       DASVTM010000386         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_355356,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000386 DASVTM010000000
VERSION     DASVTM010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355356"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VF633_10615"
     CDS             <1..227
                     /locus_tag="VF633_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX8471556.1"
                     /translation="AAGYGLQSAGFDVRGLGVGRADGGWTSFWFDRTYDGVVRIRPGR
                     TDVRVTARDLSHDRGQACRLARKIVTDMLP"
     gene            complement(242..1147)
                     /locus_tag="VF633_10620"
     CDS             complement(242..1147)
                     /locus_tag="VF633_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8471557.1"
                     /translation="MIPFVRDIDVAYGRRDQVSPLIQRVTAENPGPFTFTGTGTYIIG
                     RNEPGAGLAVIDPGPHDEAHLQALLAAVEGRRVTHVLVTHTHRDHSPLAGPFAEVTGA
                     PILAARPPVQVTHASGPLEEDEDDDFDPDVILTGGEVIAGDGWTLEAMATPGHASNHM
                     AFVLREENALFSGDHVMGWSTTVVAPPDGDMADYMNSLDAVIDREFATLWPTHGAPVT
                     EPRPFLLAYRAHRRAREAQVLDRLASGDRRIADMIPVLYAAVDRRLWPAASLSVFAHL
                     GKLVRDGRVAAEPAPALDAIWTLAD"
     gene            1235..1381
                     /locus_tag="VF633_10625"
     CDS             1235..1381
                     /locus_tag="VF633_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471558.1"
                     /translation="MVKSIAFNVSNRLQPVAAAILCLMVAQLVTASCTPEPVPYATRV
                     SINR"
     gene            complement(1382..1786)
                     /locus_tag="VF633_10630"
     CDS             complement(1382..1786)
                     /locus_tag="VF633_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEX8471559.1"
                     /translation="MNRFRTLAPDVFVAGQITGADLVVAKGRGVVRVINNRPDGEEPN
                     QPTAFELETAARELGLEYVHAPTRGMPGPDVIATVQAALLDGAPVLLYCKSGMRSAAA
                     WALASSRSGALSRDEILTGAAAAGYDLGGLPL"
BASE COUNT          300 a          575 c          624 g          311 t
ORIGIN      
        1 gtgcggcggg gtacggcctg caatcggcag ggtttgacgt gcgaggccta ggagtcggtc
       61 gcgctgacgg gggctggacc agtttctggt tcgatcggac ctatgatggc gtcgtgcgaa
      121 tccgaccggg tcgtacagac gttcgggtga ccgcccgcga cctgtcccac gaccgggggc
      181 aggcctgccg tctggcccgc aagatcgtca cggacatgtt gccctaggcc gaggcgtgac
      241 gtcagtcggc gagggtccag atcgcatcca gagcaggggc gggctcggct gcaacccgcc
      301 cgtctctcac cagcttaccc agatgggcga acactgacag gctggcggcg ggccacagtc
      361 ggcgatcgac agcggcgtag agcaccggga tcatgtccgc gatccggcga tcccctgaag
      421 ccagccgatc cagcacctgt gcctcgcgcg cccgccggtg ggcgcggtag gccagcagga
      481 aaggtctagg ctctgtcacc ggcgccccgt gggtgggcca cagggtggcg aactcgcggt
      541 caatgacagc gtcgaggctg ttcatgtagt cggccatgtc cccgtccggg ggggcgacca
      601 cggtggtcga ccagcccatg acgtggtctc cgctgaacag ggcgttctct tccctcagca
      661 cgaaggccat gtggttcgag gcgtggccgg gagtggccat ggcttcgagg gtccagccgt
      721 cgcctgcgat cacctcgccg cccgtcagga tgacatcagg gtcgaagtcg tcgtcttcat
      781 cctcttccag cggaccggag gcgtgggtga cctgcacggg aggtcgggcg gccaggatcg
      841 gcgctccggt gacctcggca aaggggccgg cgaggggaga gtggtcgcgg tgggtatggg
      901 tcaccagcac atgggtcacg cgccggcctt cgaccgcggc cagtagcgcc tgcagatgcg
      961 cctcgtcgtg cgggccggga tcgatgacgg cgagacctgc accgggctca ttgcgaccga
     1021 tgatgtaggt ccctgtgccg gtgaaggtga agggaccggg gttctccgcg gtgacgcgct
     1081 ggatcagcgg cgatacctga tcgcggcggc cgtaggcgac gtcgatgtca cggacgaagg
     1141 ggatcatcgg ggggcgccac tctcgatacc cgtcacggca agctggcccg gcccttgcct
     1201 taacaagacg tgaatccctt gccggagctg cgtcgtggtc aagtccatcg ccttcaatgt
     1261 ctcaaatcgg cttcagccgg tcgctgcggc catcctgtgt ctcatggtgg cgcaactggt
     1321 cacggcttcc tgtacgcccg agcctgttcc ctacgccacg cgggtttcga tcaatcgata
     1381 actacaacgg caatccgccc agatcatacc cggctgccgc agcgcccgtc aggatctcgt
     1441 cgcgcgacaa agcgccggac cgactggacg ccagcgccca ggcagcggcc gaccgcatgc
     1501 ctgacttgca ataaagcagt accggagcgc cgtctaagag ggcggcctgg acggttgcga
     1561 ttacgtccgg acccggcatg ccacgagtcg gggcgtggac atattcaagg ccgagctcgc
     1621 gggcggcagt ctccagttcg aaggctgtgg gctgattggg ctcctcacca tcgggtctgt
     1681 tatttatgac ccgcacaacg ccgcgcccct ttgccaccac cagatcggct ccggtgatct
     1741 gcccggcgac gaagacatca ggcgcaaggg ttcgaaaacg gttcatctac tggcgcttta
     1801 cgcggattgc
//
LOCUS       DASVTM010000387         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_92199, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000387 DASVTM010000000
VERSION     DASVTM010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92199"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..735
                     /locus_tag="VF633_10635"
     CDS             238..735
                     /locus_tag="VF633_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX8471560.1"
                     /translation="MKTQTFPFRTAALRVAAIAAPALAGAILLAAPVESVQARTPTQT
                     ASNPPVLPGYWEYTTSAVGDRNTETKCVRPQDIARFFGGISTRRYACTYPTREVGNGN
                     AHFEGTCQDKKGRRFNVRLTGTYHPENFRFNGGAQLLRGTPYIPASITARRISAQCPA
                     NAEYF"
     gene            810..1754
                     /locus_tag="VF633_10640"
     CDS             810..1754
                     /locus_tag="VF633_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEX8471561.1"
                     /translation="MPSGLIALLDDVAGIAKLAAASLDDMGAAAGKASTKAAGVVVDD
                     AAVTPRYVMGLAPARELPIIGKIAIGSIRNKLLVILPIALLLTSFAPWAITPLLMCGG
                     AYLCFEGAEKLMEMFGAEGHAEEAPVTDDLAALEKATVAGAVRTDLILSAEIMTIALA
                     DVSTAPLVTQAGTLIIVGLVMTVVVYGAVGLIVKMDDIGLHLAQRPSGAAKALGRGLV
                     KGMPIVMDALSTIGTAAMLWVGGGILLHGSHTLGLAWPAEPVEHLAHAVASALGPVGA
                     VAGWLTTALISAVIGIVVGGIVAIVLHQAMRLKPGAKH"
     gene            complement(1837..2397)
                     /locus_tag="VF633_10645"
     CDS             complement(1837..2397)
                     /locus_tag="VF633_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX8471562.1"
                     /translation="MTNKSGLEFKIGDPVVYPAHGVGKVIGVETQEVAGMSLEVYVVT
                     FDHEKMTLRVPTKKAKTAGLRSLAADDVVTKALTTLKGRARVKRTMWSRRAQEYEAKI
                     NSGDLISIAEVVRDLHRADSQPEQSYSERQLYESALDRMAREVAAANRIDKEAAVELL
                     AKSLSAKKAVIAAAEAEAEAEEAEAA"
BASE COUNT          477 a          870 c          812 g          464 t
ORIGIN      
        1 agagcaaacg gcccggacgc atgaaatgag gacgcgcaag agacggactc ttgcgcgaga
       61 gggggccgta tagaggaacg ccgggcgaga cgtcaaggct ttcagggccg aaagcgagtc
      121 tcggcgctgc ctcgggcact ctgacaacct gacggaacag cgctgttcag ccgtcgttca
      181 aaagccgcca tatagataca gggtgacatc agacacgctg accgggacat aagaaagatg
      241 aagacccaga ccttcccttt tcgcaccgcc gccttgcgcg tcgcggcgat tgcggctcca
      301 gccctcgccg gtgcgatcct gctcgcggcc ccggtcgaga gcgttcaggc gcgcacgccc
      361 acccagaccg ccagcaaccc tccggtgttg cccggctatt gggagtacac gacgagcgcc
      421 gtcggggatc gcaacaccga aaccaaatgc gtgcgaccgc aggacatcgc tcgcttcttc
      481 ggcggcatct cgacgcgacg ctacgcctgc acctatccca cccgtgaggt cggcaacggc
      541 aatgcacatt tcgaggggac ctgtcaggac aagaagggcc gcaggttcaa cgtgcgcctg
      601 accggcacct accaccccga aaacttccgc ttcaacggcg gggcccaact gctgcgcggc
      661 accccctaca tccccgccag catcaccgcc cgccgtatca gcgcccagtg cccggcgaac
      721 gcggaatact tctgaccccg ccccacatcg acaaagtgaa gaccctcctc taggcctgag
      781 acctccctcc tcagacccag cgacgcgcca tgccctccgg acttatcgcc ctgctcgacg
      841 acgtcgcggg catcgccaaa ctcgctgcgg cgtcgctcga tgacatgggg gctgcggcgg
      901 gcaaggcctc gaccaaggcg gccggcgtgg tcgtcgatga tgccgcggtg acgcctcgct
      961 atgtcatggg tctggcgccg gcgcgcgaac tgccgatcat aggcaagatc gccatcgggt
     1021 cgatccgcaa caagttgctg gtgatcctgc cgatcgcctt gctgcttaca agtttcgcgc
     1081 catgggccat cacaccgctg ctgatgtgcg gcggcgccta cctctgtttt gagggagcgg
     1141 agaaactgat ggagatgttc ggcgccgagg gtcacgccga ggaggctccc gtcaccgacg
     1201 accttgcggc tctggaaaag gcgaccgttg cgggcgccgt ccgcaccgac ctgatcctgt
     1261 ccgcagagat catgaccatc gccctggcag atgtctcgac cgccccgctg gtgacccagg
     1321 cagggaccct gatcatcgtc gggctggtca tgaccgtggt cgtctacggc gctgtcggcc
     1381 tgatcgtgaa gatggacgac atcgggctgc atctggccca acgcccctca ggcgcagcca
     1441 aggccttggg gcgcggtctg gtcaagggaa tgccaatcgt gatggacgcc ctgtccacca
     1501 ttggcaccgc cgcgatgttg tgggtcggtg gcgggatcct gttgcatggt tcgcatacgc
     1561 tcggcctcgc gtggccagcc gagcccgtcg agcatctggc ccatgccgtc gcctcggctc
     1621 tggggccggt cggggccgtc gccggctggc tgaccaccgc cctgatctcg gccgtcatcg
     1681 gcatcgtggt cggcggcatc gtcgccatcg ttctgcatca ggcgatgcgg ctgaagcccg
     1741 gcgcaaagca ttaggccgat cttccatcga gatcgcaccc agcaaaaagg cccgggatcg
     1801 ctcccgggcc ttttcagttt cgaagccccg taaggatcag gccgcttcag cttcttcggc
     1861 ttcagcttcc gcttcggcag cggcgatgac ggccttcttg gcgctcagcg acttggcgag
     1921 cagctcgacg gcggcttcct tgtcgatccg gttggcggcg gcgacttcgc gagccatccg
     1981 gtccagagcc gattcataca gctggcgttc cgaataggac tgctccggct gcgagtcggc
     2041 acggtgcagg tcgcgaacga cttcggcgat cgagatcagg tcgccggagt tgatcttggc
     2101 ttcgtattcc tgggcccgac gcgaccacat ggtgcgcttg acgcgggcgc ggcccttgag
     2161 ggtcgtcagg gccttggtca ccacatcgtc agccgcgagc gaacggaggc ccgcagtttt
     2221 ggcctttttg gtcgggacgc gcagggtcat tttttcatgg tcgaaggtca cgacgtagac
     2281 ctccagcgac atgcctgcca cttcctgggt ctcgacgccg atgaccttgc cgacaccgtg
     2341 agcaggatag acgaccgggt cgccaatctt gaattccaga ccgctcttgt tcgtcatgtc
     2401 attcctttcc cggactcagg accggagaat acgaaggcgc aggaacgcga aaaggctctc
     2461 aaccggctgc gatatcgccg ggcgaaaggt tcaggcgtca aatgcggaaa cggatcaccc
     2521 aacctctggc cgatcccgcc ttcatgggca ggattctgtc gcaaacacgg gacgcgaacg
     2581 gagttcgctc acatcccgat ccaatgaggg agaacctatc aca
//
LOCUS       DASVTM010000388         8649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_887053,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000388 DASVTM010000000
VERSION     DASVTM010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887053"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /gene="ligA"
                     /locus_tag="VF633_10650"
     CDS             <1..1609
                     /gene="ligA"
                     /locus_tag="VF633_10650"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX8471563.1"
                     /translation="AWGELSAPFADTQEHALAALASWGFKVNPRSVRVEGAQGLTAVY
                     RGLETDRAGLGYDIDGVVYKVDRLDWQDRLGFVSRSPRWAIAHKFPAQQATTVLEGID
                     IQVGRTGSLTPVARLHPVTVGGVVVKNATLHNEDEIARKDVRIGDTVVLQRAGDVIPQ
                     IVSVVLEKRPDDAVPYIFPTHCPVCGSEAVRPEGEVRRRCTGGLICSAQLVERLKHFV
                     SRRAFDIEGLGEKQLIAFHERGWIHEPADIFRLARDAEKLDLLRTEDGYGETSVANLT
                     AGIDARRVIALERLIFGLGIRDIGDQTSLLLARHFGSWADFHAAGLEAASGIPTPEWA
                     QLADGNGVSARTLSAMAGTEMSADDPWPEAPIDQKVALAFPGIATPARRALAVLAPDW
                     SSVVELARVAREEGPSETLGQLAGITGVGPVAARAIAEFFHEAHNREVVDALIAELTE
                     VVDAERPKTDSPVAGKTVVFTGSLERFTRDEAKARAESLGAKVSGSVSKKTDYVVAGP
                     GAGSKLKDAEKHGVTVLSEDAWLDLIG"
     gene            1733..2167
                     /locus_tag="VF633_10655"
     CDS             1733..2167
                     /locus_tag="VF633_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX8471564.1"
                     /translation="MKTSLFMAAALAVAALAAPALATPPSGDPTGLWQTPTNGGQVRI
                     SRCGQALCGVLVTSDHIRANPGMTDSHNRDAGQRGRTLRNLPMLNGFTGGPTEWRNGS
                     VYNPDDGRTYRGTITMQNPNSLRLRGCVVAPLCKTQTWTRVQ"
     gene            complement(2211..3200)
                     /locus_tag="VF633_10660"
     CDS             complement(2211..3200)
                     /locus_tag="VF633_10660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8471565.1"
                     /translation="MKTRTLGRSGPEVGAVGLGCMGMAAFYGQGLDESQATTVIHRAL
                     ELGVTLFDTAEMYGPHTNEIQVGKALAGKRDQAFVATKFGIGYNQDRTAMKVDGSPAN
                     VRRAIESSLKRLNMDHVDLYYLHRVDADTPIEETVGAMAELVKEGKVRFLGLSEASPE
                     TIRKAHATHPITALQTEYSLWSREPEAEILPTVRELGIGFVPYSPLGRGFLSGDIKSI
                     DDLAPDDFRRTNPRFVGDNFQKNLDLVEAVKALAAAKGLTAAQLALAWVLAQGDDLVP
                     IPGTRRIPTLEQNVAAANVVLSTDDLAAIEAVFPKDAASGERYAPAGRAALNR"
     gene            complement(3260..3649)
                     /locus_tag="VF633_10665"
     CDS             complement(3260..3649)
                     /locus_tag="VF633_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8471566.1"
                     /translation="MIVDTSALIAIIFDEPEASDLLAVLMSGRCRMSAATCFETGMVI
                     DRKAENGFRAEAFKELLVLAEIEIVPFGEDQIRLARSAYAEFGKGRHRARLNFGDCFT
                     YALAKETGEPLLFKGNDFSQTDIISAV"
     gene            complement(3646..3900)
                     /locus_tag="VF633_10670"
     CDS             complement(3646..3900)
                     /locus_tag="VF633_10670"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX8471567.1"
                     /translation="MLNIKNPETHRLAKELAERRGESLTTAVTVALREQLAREPERKP
                     KATIEEIQALLQEMRKHLKPLDPGEDPTAFLYDPETGMPA"
     gene            complement(3935..5746)
                     /locus_tag="VF633_10675"
     CDS             complement(3935..5746)
                     /locus_tag="VF633_10675"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX8471568.1"
                     /translation="MRQSFDETTDPSFGAQHLPLVRARLAEQGLDGFLVPHEDEHQNE
                     YLPEANDRLAWLTGFTGSAGAAVVLSDRAAVFADGRYTVQVRAQVDPHQFEILDLVEG
                     GVPAYLAGASKGAVIGYDARLHSPDALATLSLAATKAGATLKSVDLNPLDQAWGDARP
                     AQPQAPVVPHEDRYSGESSASKRARIGRAVTAAGADVAVLTAPSSIAWLFNVRGGDVI
                     RSPLPLGQAIVAADGTAQLFLDPAKVTNELPGWLGDEVALKPAAHVDEALGEQRGRKV
                     LIDPAQSSAWYFDRLETAGAEIVRAMDPCAIPRAAKNPVEIEGSRQAHIRDGAALTRF
                     LHWVGTTAQETLPDEREIAGILEGFREATGALKDLSFDTIAGAGPNGALPHYKPVGDK
                     IRRIEKGSLLLVDGGGQYLDGTTDVTRTMAIGDPTPDQIRMFTLVLKAHIAMAVIRFP
                     VGTTGHQLDAIARAPMWMAGLDYDLGTGHGVGSYLGVHEGPQRIAKAVNTQPLLTGMI
                     LSNEPGYYREGHWGIRIETLQVVTAPEPIPGGERSMHGFEQLTFAPLDRRLIDVELLT
                     EAERAYVDAYHAETLAKVGPLLDGEALDWLKGQAAPL"
     gene            complement(5809..6981)
                     /locus_tag="VF633_10680"
     CDS             complement(5809..6981)
                     /locus_tag="VF633_10680"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX8471569.1"
                     /translation="MSGELETAALDSAGGWLRWKRKHADIPVGTPCANCEVPLQGTYC
                     YNCGQLAEDFHRNSWHLLVEAVESLLHLDGRLFKTLPGLIWRPGQLTRDYLEGKRAWQ
                     VQPFRMFLVILLIVLFVGHLATKSAEHKAEAQGGDHSTTNLVVPADSAAASDLEKARA
                     QIQADPDMSASEKAAALAATRGDWAAFSAHIAENARETAAADDGHRPASSPPPAPSAA
                     TATATADGENLHVNSRILEGDDPRLQGFKHWAEARLTAVKDDPERFALIMEIWLHRVA
                     ILALPVSALMLSLLFIFNRRYFVFDHLIFSMHSLSFQMLLVTVILLLSMIVGGAAWWL
                     ILIMPVHLYVHMKGAYQRSAFGTVARMSALFFLTNFTFGLLALLWLYLAFNEMAGH"
     gene            complement(7002..7904)
                     /locus_tag="VF633_10685"
     CDS             complement(7002..7904)
                     /locus_tag="VF633_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8471570.1"
                     /translation="MTDQYPTETDLNASDAANAVADSNPINQPLVIDPAPDADNDLVL
                     IESTTDGVVFVTINRPAKKNAFDAATIAALHEAFLTLHGADTVRVVYLRGAGGTFSAG
                     ADLAWMRSAADWSESDNRDDAMGLAKMLKALHDVPALTVAIVEGAAMGGGAGIVAACD
                     MAVAVEGAQFAFSEVKLGLIPATIAPYVVEAVGARRARALFLTGNTFDADYAAHLGLI
                     DMVLPDAESIDSFVAMLSDSLTGNAPQAMGAAKALVHHVSTHKLDSSLMEDTAKRIAR
                     ARVSDEGQEGVRAFLEKRKPNWAV"
     gene            8049..8486
                     /locus_tag="VF633_10690"
     CDS             8049..8486
                     /locus_tag="VF633_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX8471571.1"
                     /translation="MSLTGAYDPSNIFAKILSGDIPSAKVWEDDDVLAFMDVFPQSEG
                     HVLVISKTSTAQNLLEIEPDVLAKLTAAVQRTARAVTKALEPEGFSILQFNGEAGGQT
                     VFHLHFHIIPRWADREMKGHGHAPMADAAALKMLARKIAAQLE"
BASE COUNT         1552 a         2812 c         2794 g         1491 t
ORIGIN      
        1 tgcctggggc gagttgtcgg cgccgttcgc ggacacacag gaacacgctc tggccgcgct
       61 cgcgtcttgg ggtttcaagg tcaatccgcg ctcggtccgc gtcgagggtg cgcaaggact
      121 gacggccgtc tatcgcggac ttgagaccga tcgcgcaggc cttggctacg acattgacgg
      181 cgtcgtctat aaggtcgacc ggctggactg gcaggaccgt ctcggcttcg tctcgcgcag
      241 cccccgctgg gccatcgccc acaaattccc tgcccaacag gcgaccaccg tgctggaggg
      301 catcgacatc caggtcggcc gcacgggttc cctaactccg gtcgcgcggc tgcatccggt
      361 caccgtcggc ggcgtagtgg tcaagaacgc gaccctccac aacgaagacg agatcgcccg
      421 caaggacgtc cgcatcggcg acaccgtcgt cctgcagcgc gcaggcgacg tgatcccgca
      481 gatcgtctcg gtggtgctgg agaagcgccc ggacgacgcc gtcccctata tcttcccaac
      541 ccactgcccg gtgtgcggtt cggaggctgt gcgacccgag ggcgaggtcc gtcgtcgctg
      601 caccggcggc ctgatctgca gcgcccaact ggtcgaacgc ctcaagcatt tcgtctcgcg
      661 tcgcgccttc gacatcgagg gtctgggcga gaaacaactg atcgctttcc acgaacgcgg
      721 ctggatccac gaacccgccg acatcttccg tctggcgcgt gatgcggaaa aactggacct
      781 tctgcggacc gaggacggct atggcgagac cagcgttgcg aacctgaccg caggcatcga
      841 cgcccggcgg gtgatcgcgc tggagcggct gatcttcggc ctcggcattc gcgatatcgg
      901 cgaccagaca tcgctgctgc tggcccgcca tttcggaagc tgggcggatt tccatgccgc
      961 cgggctcgag gccgcctcgg gcattccaac gccggaatgg gcgcaactgg ctgatggaaa
     1021 cggtgtgtcc gcacggacct tgtcagcgat ggcggggacg gagatgtctg cggatgatcc
     1081 ctggcccgaa gcgccgatcg atcagaaggt ggccctcgcc ttccccggca tcgcgacccc
     1141 ggcgcgccga gctttggccg tgttggcgcc cgactggtca tcggtcgtcg aactggcacg
     1201 agtcgcgcgc gaagagggtc cgtccgaaac gctgggccaa ctcgcaggca tcacgggagt
     1261 aggcccggtc gccgcccgcg ccatcgcgga gttcttccac gaagcccata accgcgaagt
     1321 cgtcgacgcc ctgatcgccg agctgaccga ggtcgtcgat gcagaacggc ccaagactga
     1381 cagcccggtc gcgggcaaga ccgtggtttt caccgggtcg ctggaacggt tcacccgcga
     1441 cgaggccaag gcccgcgccg agagcctcgg cgccaaggtg tcgggatcgg tgtccaagaa
     1501 gaccgattac gtcgtcgccg ggcccggagc gggctccaag ctgaaggatg cggaaaagca
     1561 tggggttacg gtcctcagcg aagacgcgtg gctcgatctg attggctgaa acttgacgta
     1621 agggccggtt tttattggcg atagggccga tctgcagcct tgtttcgacc ggaaacgcgg
     1681 gtcagtctgg gttcaagcga tcgacgctac gatccgaacc aaggaaacga tcatgaagac
     1741 ctctctgttc atggctgccg cgctggctgt cgccgcactc gcagcccccg cgctggctac
     1801 cccgccgtcc ggtgatccga ccggcctgtg gcagacgccg accaacggtg gtcaggtgcg
     1861 cattagccgt tgcggtcagg ccctgtgcgg cgttctggtg acctcggacc acatccgcgc
     1921 caaccccggc atgaccgaca gccacaaccg cgacgccggc cagcgcggtc gcaccctgcg
     1981 caacctgccg atgctgaacg gcttcaccgg cggtccgaca gaatggcgca acggctcggt
     2041 ctacaacccc gacgacggcc gcacctatcg cggcaccatc acgatgcaga accccaatag
     2101 cctgcgtctg cgcggctgcg tcgtcgcccc gctgtgcaag acccagacct ggacgcgggt
     2161 tcagtagcaa cgaaaaccgc cgccggaccc ggtccggcgg cggtaacgtc ctagcggttc
     2221 agagctgccc ggccggctgg agcgtaacgc tcgccggagg ctgcatcctt agggaagaca
     2281 gcttcgatgg cggccagatc gtcagtcgac agaacaacat tggccgcagc aacattctgc
     2341 tccaacgtcg gaatgcgacg agtcccgggg atcgggacca gatcatcgcc ttgcgccagc
     2401 acccaggcca gagccagctg cgccgccgtc agacccttgg ccgccgccag cgccttgacc
     2461 gcctcgacga ggtcgaggtt cttctggaaa ttatcaccga cgaaacgcgg attcgtccgg
     2521 cggaagtcat cgggcgcaag gtcatcgatc gacttgatat cgcctgacag gaagccccga
     2581 ccgagcgggc tatagggcac gaagccgatg ccgagctcgc ggacggtcgg caagatttcc
     2641 gcctccggct cgcggctcca cagcgaatat tcggtctgaa gcgcggtgat cgggtgagtg
     2701 gcgtgggcct tgcggattgt ctcaggcgac gcctcggaca gcccgaggaa gcggaccttg
     2761 ccctccttga ccagctcggc catcgccccg accgtctcct cgatcggcgt gtcggcatcg
     2821 acgcggtgca gataatagag gtcgacgtgg tccatgttga gccgcttcag gctgctctcg
     2881 atcgcgcgac ggacgttggc gggcgagccg tcgaccttca tggccgtgcg gtcctgattg
     2941 tagccaatgc cgaacttggt cgcgacaaag gcctgatcgc gcttgcccgc gagcgccttg
     3001 ccgacctgaa tttcgttggt gtgggggcca tacatctcgg cggtgtcgaa cagggtgacg
     3061 ccgagctcga gcgcgcggtg gatcaccgtc gtcgcctggc tctcgtcgag gccctggcca
     3121 taaaacgcgg ccatacccat gcagccgaga ccgacagcgc cgacctccgg gcctgatctt
     3181 ccaagcgtgc gggtcttcat ggggcgctcc tgcgattgtg agaggctgag gtcgggtgga
     3241 ggctgtgaac ccgcaagggt cagacggcgg aaatgatgtc ggtctgtgag aaatcgttgc
     3301 ccttgaacag cagcggctcg ccggtttcct tggcgagggc gtaggtgaag cagtcgccga
     3361 aattcagtcg cgcccgatgg cgacctttgc cgaactcagc ataggcgctg cgggcgagcc
     3421 gaatctgatc ttcgccgaag ggcactatct cgatttcggc cagtaccagc agctctttga
     3481 aggcctcggc gcgaaatccg ttctccgcct tccggtcaat aaccatgccg gtttcaaaac
     3541 acgttgccgc tgacattcgg catcggccgc tcatcaacac tgcaagcaaa tccgatgcct
     3601 cgggttcatc gaagatgatc gcgatgagcg ccgaggtatc gacgatcaag ccggcattcc
     3661 tgtctcggga tcgtagagaa aggccgttgg atcttcgcca ggatcaagcg gcttcagatg
     3721 ctttctcatc tcctgaagca gggcttgaat ttcctcaatg gtcgccttgg gcttccgctc
     3781 aggctcgcgc gccaactgct cacgcaaggc gacggtcacg gcggtcgtca ggctttcgcc
     3841 acgccgttcg gccagttcct tcgccagacg atgggtctcg gggttcttga tattcaacat
     3901 ggtacaaacg cttctaccat gtctcgcaag acgctcaaag cggtgcggcc tgccccttca
     3961 gccagtccag cgcctcgcca tccaatagcg gccccacctt ggccagcgtc tccgcgtgat
     4021 aggcgtcgac ataggcacgc tcggcctcgg tcagcagctc gacgtcgatc aggcggcggt
     4081 ccaacggggc gaatgtcagc tgctcgaacc catgcataga ccgctcgccg cccggaatcg
     4141 gctcgggcgc ggtgacgacc tgcagcgtct cgatgcggat gccccagtgg ccttcacgat
     4201 agtatcccgg ctcgttggac aggatcatcc cggtcagcag cggctgggta ttgaccgctt
     4261 tggcgatgcg ttgcggacct tcgtggacgc cgagatagct gccgacgccg tgaccggtgc
     4321 cgaggtcgta atcgaggccc gccatccaca tcggcgcacg cgcgatggcg tccaattggt
     4381 ggcccgtggt gccgaccggg aagcggatca cggccatggc gatgtgggcc ttgagcacga
     4441 gggtgaacat gcggatctgg tccggcgtcg ggtccccgat ggccatggtg cgcgtcacgt
     4501 cagtggtgcc gtccagatac tggccgccgc cgtcgaccag caacagcgag cccttctcga
     4561 tccggcggat cttgtcaccg acgggcttgt agtgcggcag ggcgccgttc gggccggccc
     4621 cggcgatggt gtcgaacgac aggtccttga gcgcaccggt ggcctcgcgg aagccttcaa
     4681 ggatacctgc gatctcgcgc tcatccggca aggtctcctg cgcggtcgtg ccgacccagt
     4741 gcaggaagcg tgtcagggcc gcgccgtcgc ggatatgggc ctgacgactg ccctcgatct
     4801 cgaccgggtt cttggcggcg cgaggaatag cgcacgggtc catagcgcgg acgatttcag
     4861 caccggcggt ttcgagccgg tcgaaatacc aggccgatga ctgcgccggg tcgatcagca
     4921 ccttacggcc ccgctgctcg cccagcgcct catcgacgtg ggcggcgggt ttgagcgcca
     4981 cctcgtcgcc cagccagccc ggcaattcgt tagtcacctt ggccgggtcg aggaacagct
     5041 gggccgttcc atcggcggcg acgatggcct gcccgagcgg caacggtgag cggatcacgt
     5101 cccctccccg cacattgaac agccaggcga tcgacgacgg cgcggtcaag acggcgacgt
     5161 ccgcaccggc tgcggtgacg gcccgcccga tgcgagcgcg cttcgacgca ctgctttcgc
     5221 cggaataacg atcctcgtgc ggcacgacgg gggcctgcgg ctgagccgga cgggcgtcgc
     5281 cccaagcctg atccagcgga ttgagatcga ccgatttgag cgtggcgccg gccttggtgg
     5341 ccgcaaggct cagggtcgcc aaggcatccg ggctatgcag ccgggcgtca tagccgatga
     5401 ccgctccctt ggaggctccg gcgagatagg ccgggacgcc accctcgacc agatcgagga
     5461 tctcgaactg gtgcggatcg acctgagccc ggacctgcac cgtgtagcgg ccatcggcga
     5521 acaccgcagc gcggtcgctc agcaccacgg cggcgcccgc cgagccggtg aagccagtca
     5581 gccaggcgag gcggtcgtta gcctcgggta ggtattcgtt ctgatgctcg tcctcgtgcg
     5641 ggaccaagaa gccatcgagt ccctgttctg ccagccgagc gcgaacgagc ggaaggtgct
     5701 gggccccgaa ggacggatcg gtggtttcat cgaaggactg gcgcatggcg cagacctagg
     5761 ccccgtcgcc gatctgctca agcccaccat gacgtcaacg cccgtgcttc agtgccccgc
     5821 catctcgttg aaggcgaggt aaagccagag cagggccaac aggccgaacg tgaaattggt
     5881 cagaaagaac agcgcggaca tgcgagcgac ggtgccgaat gcgctgcgct gatacgcgcc
     5941 cttcatgtgc acatacaggt gcaccggcat gatcaggatc aaccaccagg ccgcgcctcc
     6001 gacgatcatt gaaagcagca ggatcactgt caccagcaac atctggaacg acagcgagtg
     6061 catcgagaag atcagatggt cgaacacgaa atatcgcctg ttgaagatga acagcaggct
     6121 tagcatcaac gccgagaccg gcagggcgag gatggcgacc cggtgcagcc agatttccat
     6181 gatcagggca aagcgctccg gatcgtcctt caccgctgtc agccgggctt cggcccaatg
     6241 tttgaagcct tggaggcggg ggtcgtcgcc ttccagtatt ctggagttca cgtgaaggtt
     6301 ttcgccgtct gcagtcgcag tcgccgtcgc cgccgaggga gccggcgggg gcgatgatgc
     6361 aggtctgtgg ccatcgtcag ccgctgcagt ctcacgggcg ttctcggcaa tgtgggcgct
     6421 gaaggcggcc cagtctcccc gcgtcgccgc gagggcggca gccttctcgg aggccgacat
     6481 gtcggggtcg gcctggatct gggcgcgagc cttctcaaga tcggaggctg cggcgctgtc
     6541 agcaggaaca accagattag tggtgctgtg gtcgccgcct tgagcctcgg ccttgtgctc
     6601 agcagacttg gtcgcgaggt ggccgacgaa caggacgatc agcaggatca cgaggaacat
     6661 gcggaacggc tggacctgcc aggcgcgctt accctccagg tagtcccgcg tcagttgacc
     6721 cggtcgccag atcaggcccg gcagcgtctt gaacaaccgt ccgtcgagat gcagcagact
     6781 ctccaccgcc tcgaccagca ggtgccagga gttgcggtgg aaatcctcgg ccagttggcc
     6841 gcagttgtag caataggtgc cctgaagcgg gacctcgcag ttggcgcacg gggtgccgac
     6901 cgggatgtca gcgtgcttgc gcttccacct cagccagcca ccggcggagt ccagcgccgc
     6961 cgtttccagt tcccctgaca tcacgccccc tgaagtagaa tctacacagc ccagtttggt
     7021 ttacgttttt cgaggaaggc gcgcaccccc tcttgaccct catcagacac gcgagcgcgc
     7081 gcgatgcgtt ttgccgtgtc ttccatgaga ctgctatcga gcttgtgggt cgaaacgtgg
     7141 tgcaccaacg ccttggccgc gcccatcgct tgcggcgcgt tgccggtcag gctgtcgctg
     7201 agcatggcca cgaagctgtc gatggattca gcgtcgggca ggaccatgtc gatcaggccg
     7261 aggtgcgctg catagtccgc gtcgaacgta ttcccggtca ggaacagcgc acgagcgcga
     7321 cgcgcgccga ccgcctcgac gacatagggt gcgatggtcg ccgggatgag gcccagcttg
     7381 acctcggaga aggcgaactg cgcaccttcg accgccacgg ccatgtcgca agcggcgacg
     7441 atgcctgctc cgccgcccat tgccgcgcct tcgacgatcg ccacggtcaa agccggaacg
     7501 tcgtgcagcg ccttgagcat cttcgccagc cccatggcgt cgtcgcgatt gtcgctctcg
     7561 gaccagtcgg cggccgaccg catccatgcg aggtcggccc cggcgctgaa cgtgccccct
     7621 gccccacgca gatagacgac ccgcaccgta tcggcgccat gcagtgtcag gaacgcctcg
     7681 tgcagggcgg cgatggtcgc ggcgtcgaag gcgttcttct tcgccggacg gttgatggtg
     7741 acgaacacaa cgccgtcggt ggtcgattcg atcaggacca gatcattgtc ggcatcgggc
     7801 gccggatcga tgaccagagg ctggttgatc gggttggaat ccgcgacggc gttagccgca
     7861 tcgctggcgt tgaggtcggt ctcggtgggg tactggtcag tcatgggatc ctcgttctct
     7921 ggcactcaaa cgcccgagcg cggctttcag tctgacttag ggcgaaagat cgccccggca
     7981 aagctcctca atcgtcccac ccgttgctat caaggcgaca ccactcgact ggcctgacgg
     8041 agacgacgat gagcctgacc ggcgcctatg acccctctaa catcttcgcg aagatcctga
     8101 gcggcgacat cccgtcggcg aaggtgtggg aggacgacga tgtcctcgcc ttcatggacg
     8161 tgttccccca gtccgagggc catgtgctgg tgatctccaa aacgtcgacg gcgcagaacc
     8221 tgctggaaat cgaacccgac gtgctggcca aactaaccgc cgccgtccaa cgcaccgccc
     8281 gcgccgtgac caaggccctc gagccagaag gcttcagcat cctgcagttc aacggcgagg
     8341 cgggcggaca gaccgtcttc cacctgcatt tccacatcat cccgcgctgg gccgatcggg
     8401 aaatgaaggg ccatggccac gcaccgatgg ccgacgctgc cgcactgaaa atgcttgccc
     8461 ggaagatcgc cgcccaactg gaatgatgtc gccagtcgca gcggtcgcag cgggatgaac
     8521 aggaaaaggc ttcaccagat agccaagaag cttgcccacg agagattccg ctatcataac
     8581 ccgcgccgga tgcggcttga cccatcccgt gccgtcacgg caggccggct tagctcagtt
     8641 ggtagagcg
//
LOCUS       DASVTM010000389         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_518460,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000389 DASVTM010000000
VERSION     DASVTM010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518460"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1141)
                     /locus_tag="VF633_10695"
     CDS             complement(155..1141)
                     /locus_tag="VF633_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8471572.1"
                     /translation="MRHDKAMMVIELARRMAASAEGLTIDEMAREARVGRRTAERLRD
                     AVLMLFPAVEEVSDPPTKRWRIRGGLSAFEQAPTTTELVELSKAAQQLRTTGETGRAA
                     ALEGLERKLKSAMRSTTLNRLAPDLEALVRAETIAVQAGPRPSADENVLATIRGAVLA
                     QQPLGFTYARPGAEPRRRSVAPCGVMFGRANYLVAADRESGRIQTFRLDRMSALEAQN
                     GVAPPPADFDLTVFASQSFGIYQDEIEEVVLAVSPVGAAEARSWRWHPTQTLEDQPNG
                     GVIVRFRASGMRELAWHLFSWGEQVTIVAPARLKAVMAGELAAAQRALDAAP"
     gene            1232..1504
                     /locus_tag="VF633_10700"
     CDS             1232..1504
                     /locus_tag="VF633_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471573.1"
                     /translation="MARFASPDRFASNASFAPHAAVNAGRYMIVPCEADGCGDLAVIW
                     DRLEEQVVDVISAAASARYSDEDALWDEARGFNDQPDYAAEYRMAA"
     gene            complement(1509..1982)
                     /locus_tag="VF633_10705"
     CDS             complement(1509..1982)
                     /locus_tag="VF633_10705"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEX8471574.1"
                     /translation="MTYRILPLSLEPFANLFALSDDELLDLGARRMTAEKPNSSPCRV
                     SLCDAEPGEQLILAHHVHVEDPSSPYRSGGAIFIREGASEASLSADTVPEMLSRRQLS
                     VRAYDDAAMMVSAEVVEGVVLHDRLLHWFADPSVSDVHIHTANRGCYLARADRAD"
     gene            2088..>3229
                     /locus_tag="VF633_10710"
     CDS             2088..>3229
                     /locus_tag="VF633_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX8471575.1"
                     /translation="MNAPFKAPTVPSNEPPLWDLSDLYSSRQDARIAADLDRGRAAVA
                     ELNTLQGSLIGSRADPAVLGAKLAKAIALYEQATEALGGLGAYAFLAASTARDDAEAN
                     GFEADIREKMTAIATPTVWLTLEINQLEDAEIDAALTADADAATWRPWLRRVRAMKPH
                     ELSQELETYLAEHSPISAQWSRLFDETLAALRADVGKEKLTLAETLNRLSDPKAARRK
                     AAAEGLSTALAARASTMALVMNTTAADKALEDKWRGFKRPADGRHLGNEVHGEAVDAM
                     AEAVVAAYPRLSHRYYALKAKAMGKTTLDQWDRNAPLETSAPRGFDWLQGKAMVLDSF
                     ADLGPEFADRAGRFFDNPWIDGRARPGKQSGAYAHPVTADRHPYVFL"
BASE COUNT          552 a         1147 c         1016 g          514 t
ORIGIN      
        1 cggcgagcat tagaacggcg gcgaaaggtg ggatggatcg cattggggct ccgagggtcg
       61 aagtcacagg tacgggctcc cctgcggacc gtcacctgtg acttcgctca ttcagcaggc
      121 gggataaggg ccagatttgt ccgggcgttc gtcttcaggg cgccgcgtcc aaagctcgct
      181 gggctgcagc cagttccccc gccatgactg ctttcagacg cgctggagcg acgatggtga
      241 cctgctcgcc ccagctgaac aggtgccagg ccagctcccg catgcccgag gcccgaaacc
      301 gaacgatgac cccgccgttc ggttggtcct ccagcgtctg ggtcgggtgc cagcgccacg
      361 atcgcgcttc ggccgctcca acgggcgaga ccgccagcac cacctcttcg atctcgtcct
      421 gatagatgcc gaacgactgg ctggcgaaga cggtcagatc gaagtcggcg ggcggcggcg
      481 cgaccccgtt ctgggcctcc agcgcgctca tccgatcgag gcggaaggtc tggatccggc
      541 cgctctcccg gtctgccgcc accagataat tggcgcgacc gaacattaca ccacatggcg
      601 cgacgctgcg tcggcgcggc tcggcgcccg gtcgcgcata ggtgaatccg agcggttgct
      661 gcgccagcac ggcgccgcga atggtcgcca gcacgttctc gtcggctgac gggcgcggcc
      721 cggcctgcac cgcgatcgtc tccgcccgca ccaacgcctc gagatcggga gcgagccggt
      781 tcagcgtcgt cgaccgcatg gctgacttca gcttgcgctc cagaccctcc agcgccgccg
      841 ccctcccggt ctcaccggtg gtgcgaagct gttgcgccgc cttggacaat tcgaccagtt
      901 cggtcgtggt cggcgcttgc tcgaacgccg acagaccgcc ccggatacgc cagcgtttcg
      961 tcggcggatc gctgacctcc tcgacggcgg gaaacagcat cagcacggcg tctcgcagcc
     1021 gttcggcagt acgcctgccg acccgagcct cccgcgccat ttcgtcgatc gtcagcccct
     1081 cggccgaggc cgccatccgg cgtgccagtt cgatgaccat catcgccttg tcgtgccgca
     1141 tcgcaggcct cccgaaagat acgtccgatt ttggcgcagc gccgtcgcat tgtccatcat
     1201 cggcaagaga caccgcacag gaactcacga catggcccgc ttcgcttccc ccgaccgctt
     1261 cgcttccaac gcctccttcg ctccccacgc cgccgtcaac gccggccgct acatgatcgt
     1321 cccatgtgag gctgacggct gcggcgatct ggccgtgatc tgggaccggc tcgaggaaca
     1381 ggtcgtcgac gtcatctccg ccgccgccag cgcccgctac tccgatgaag acgccctgtg
     1441 ggacgaagcg cgcggcttca acgaccagcc tgactacgcg gccgagtatc ggatggccgc
     1501 ctgacgcctc agtcggctcg gtccgcgcga gccaggtagc agccacggtt ggctgtgtgg
     1561 atatgcacgt cgctgaccga ggggtcggcg aaccaatgca acagcctgtc atgcaggact
     1621 acgccctcga cgacttcggc gctgaccatc atcgctgcat cgtcataggc ccgcaccgac
     1681 agctggcggc gcgacaacat ctcagggacc gtgtccgccg aaagcgacgc ctccgacgcc
     1741 ccttcccgta tgaagatcgc cccaccggac cgataaggtg acgacgggtc ctccacatgg
     1801 acgtgatgcg cgaggatcaa ctgctcgccg ggttccgcat cgcaaaggct gacacgacac
     1861 ggcgaactgt tcggcttttc cgccgtcatg cgacgcgcgc cgagatcgag aagctcgtcg
     1921 tccgacaagg cgaacaggtt cgcgaaaggc tcgagtgaca ggggcaggat gcggtaggtc
     1981 atgacgggtc tccgatggct gataccgatc gatacgccgc tccgcccgtg acatctggcg
     2041 tctttcggac gttgcgccaa aaccttccaa gccccacatt tgagggcatg aacgcgccct
     2101 tcaaagcccc gaccgtcccc tcgaacgagc ccccgctgtg ggatttgtcg gacctatact
     2161 cctccagaca ggacgcgcgg atcgcagccg atctggaccg gggacgcgcc gccgtcgccg
     2221 aactgaacac cctgcagggc tccctgatcg gctcccgggc cgatcccgcc gtcctcggcg
     2281 caaagctggc caaagcgatc gcgctctatg aacaggcgac cgaagcctta ggcggtctgg
     2341 gcgcctacgc cttcctcgcc gcctctaccg cgcgtgacga cgccgaggcc aacggcttcg
     2401 aagccgacat ccgcgagaag atgaccgcca tcgccacccc gacggtctgg ctgacactgg
     2461 agatcaatca gcttgaggac gccgagatcg acgccgcgct cacggccgat gcggatgcgg
     2521 cgacctggcg tccatggctg cgtcgggtgc gggcgatgaa gccgcacgag ttgtcgcagg
     2581 agctcgaaac ctacctggcc gagcacagcc cgatctcggc gcaatggagc cggctgttcg
     2641 acgagacgct ggccgccctg cgcgctgatg tcggcaagga aaagctgacc ctcgccgaaa
     2701 cgctcaatcg gctgtcggac ccgaaggccg cgcgacgcaa ggccgccgcc gagggtctga
     2761 gcacggcgct ggccgcccgg gcctcgacca tggcgctggt gatgaacacc accgccgccg
     2821 acaaggcgct tgaggacaaa tggcgcggct tcaagcgtcc cgccgacggt cgccacctcg
     2881 gcaacgaggt ccacggcgag gcggtcgatg ccatggccga ggccgtcgtg gccgcctatc
     2941 cgcgcctgtc gcaccggtat tatgcgctca aggccaaggc catgggcaag acgacgctcg
     3001 accagtggga ccgtaatgcg ccgctggaaa cctctgcccc gcgcggcttc gactggctgc
     3061 aaggcaaggc gatggtgctc gacagtttcg ccgacctcgg cccggagttc gccgaccgcg
     3121 ccggtcgctt cttcgacaat ccatggatcg acggtcgcgc gcgtccgggc aagcagtcgg
     3181 gtgcttatgc gcacccggtg acggccgatc gccaccccta cgtcttcct
//
LOCUS       DASVTM010000390         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_887149,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000390 DASVTM010000000
VERSION     DASVTM010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887149"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VF633_10715"
     CDS             complement(<1..227)
                     /locus_tag="VF633_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471576.1"
                     /translation="MNRPVFAAVTGLALLAVLGGSAVAQSAGGGRTNVSNQPIMVGAD
                     GGERTNTGFALRGRAEVTQGQNRLRADSIVG"
     gene            complement(236..913)
                     /gene="lptC"
                     /locus_tag="VF633_10720"
     CDS             complement(236..913)
                     /gene="lptC"
                     /locus_tag="VF633_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX8471577.1"
                     /translation="MPEAESPDQDAVEARRLSDETRLAEAASKWRARSRRVQLYRRLL
                     PILIVVLAGGALTWTVFRTVMSGVERKASQSREVRLDNPMFHGQDAQGRSFLIGAQGA
                     VRDPNTGHFRLNGPVLRLNLGGRKVTQMTADAGIYDEAKRTVTIGPNVKITDGGSGFT
                     LVTPEAVVDTATGITTGNKGIQGHGPLGTISASSYAIYDQGERVTFNGSGDNKIRGVF
                     NTTRSGK"
     gene            complement(906..1520)
                     /locus_tag="VF633_10725"
     CDS             complement(906..1520)
                     /locus_tag="VF633_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8471578.1"
                     /translation="MTVYFHEGDLPDGLDLGLEVAVDSETMGLRYHRDPLCVVQLSDG
                     KGDAHVVRMRRPAYDCPNLKALMADRSVLKIFHFGRFDIAMMQLYLGVETGPVYCTKI
                     ASKLARTYTDRHGLKDVTRELAGVDLSKAQQSSDWGADVLTQAQLEYAASDVLYLHAV
                     KTRLDMMLAREGRTELARACFDFLPTRSRLDLEGWEETDIFAHA"
     gene            1671..2093
                     /locus_tag="VF633_10730"
     CDS             1671..2093
                     /locus_tag="VF633_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471579.1"
                     /translation="MGNPWLMAAGGLNAVASLVHLGCIVGGAPWYRFFGAGEGMARLA
                     ERGSMKPTLITLCIAGILALWSICAFSGAGILPRLPLLKPALIAITVVYLLRAAALPL
                     MLRTVPDLGWTFLFVSSAIVLAIGLIHAIGLWTGWRTL"
     gene            complement(2256..2341)
                     /locus_tag="VF633_10735"
     tRNA            complement(2256..2341)
                     /locus_tag="VF633_10735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2306..2308),aa:Leu,seq:cag)
     gene            2506..>2831
                     /locus_tag="VF633_10740"
     CDS             2506..>2831
                     /locus_tag="VF633_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8471580.1"
                     /translation="MSAFSPGLVTVFGGSGFVGAQAVRALAKAGWRVRVAVRSPNTSY
                     DLRPLGDVGQIQPVRCDVTSAADVAAALKGANAAVNLVGILYESGKRSFEVMHVDGAR
                     TIAEACA"
BASE COUNT          488 a          931 c          889 g          523 t
ORIGIN      
        1 ctgccgacga tggagtcggc gcggagccgg ttctggccct gggtgacttc tgcgcggccg
       61 cgcagcgcaa agcccgtgtt ggtgcgctcg ccgccgtccg cgccgaccat gatcggctgg
      121 ttgctgacgt tggtccggcc ccctccggcg ctttgggcaa cggctgatcc gccgaggacc
      181 gccagaagcg ccagacccgt gacagctgca aataccggtc tgttcatcgt cattctcatt
      241 tacccgagcg cgtcgtgttg aacacgccgc gaatcttgtt gtcgccggag ccgttgaagg
      301 tcacccgctc gccctgatca tagatggcat aggacgatgc ggatatggtt ccaagagggc
      361 cgtgcccctg gatgcccttg ttgccggtcg tgatgcccgt cgccgtgtcg accaccgcct
      421 ctggcgtgac cagcgtaaag cctgacccgc cgtccgtaat cttgacgttc ggaccgatgg
      481 tcaccgtgcg cttcgcctcg tcatagatgc ccgcgtctgc ggtcatctgc gtcaccttgc
      541 ggccgccgag attgagcctg agcaccgggc cgttcagccg gaaatgtcct gtattcggat
      601 cccgaaccgc gccctgggcg ccgatcagga atgaccgtcc ctgcgcgtcc tgaccgtgga
      661 acatcggatt atcgagacgc acctcgcggc tctggctcgc cttgcgctcg acgccggaca
      721 tgacggtgcg gaacaccgtc cacgtcaagg ccccgccggc cagcaccacg atcaggatcg
      781 gtagcaggcg ccgatacagc tgcacccgcc gcgagcgcgc acgccatttg gacgcggcct
      841 ccgccagacg tgtttcgtcg gacagacggc gcgcctctac ggcatcctga tctgggcttt
      901 cggcctcagg catgcgcgaa gatatccgtc tcttcccagc cttcgaggtc cagacgcgaa
      961 cgggtcggca ggaagtcgaa acaggccctg gccagttcgg tgcgaccctc acgcgccagc
     1021 atcatgtcca gcctggtctt cacggcatgc aaatacagga cgtccgacgc ggcgtattcg
     1081 agctgcgcct gcgtcaggac atccgcgccc caatccgagg attgctgcgc cttggacagg
     1141 tccaccccgg ccagttcgcg cgtgacgtct ttcagaccat gacgatccgt ataggtgcgg
     1201 gccagcttgg aggcgatctt ggtgcaatag accggcccgg tttcgacgcc gagatagagc
     1261 tgcatcatgg cgatgtcgaa acggccgaag tggaagatct tgagcaccga acggtccgcc
     1321 atcagggcct tcaggttcgg gcaatcgtaa gctggccgac gcatccgcac gacatgggcg
     1381 tcgcccttcc cgtcggacaa ctggaccacg cacaacggat cgcggtgata ccgcaggccc
     1441 atcgtctcgg aatcgacagc gacctcaagg ccgaggtcga ggccatcggg cagatcgcct
     1501 tcgtggaaat aaacggtcat gcggggcgag tctggctgaa gatcatggct gcaacataga
     1561 ccatgagcgg catatcgcta ggacgcgggc agccgaaagc acgacgagcg ccattactgc
     1621 gctccaaagc agcgctttcg tgcaaggatg ccccgaaacg gagggcgaag atgggcaatc
     1681 catggctgat ggcggcgggc gggctgaacg ccgtcgcgtc cctggtgcat ctgggctgca
     1741 tcgtcggcgg cgcgccctgg taccgattct tcggcgcggg cgagggcatg gcccggctgg
     1801 ctgagcgcgg atcgatgaaa ccgacgctga tcaccctgtg catcgcggga attctggccc
     1861 tgtggtcgat ctgcgcgttc tccggcgcgg ggattctacc tcgcctgccg ctgctcaagc
     1921 ccgcgctgat cgcgatcacc gtcgtctatc tgctgagagc cgctgccctg ccgctcatgc
     1981 ttagaaccgt gcccgatctc ggctggacct tcctgttcgt cagctccgcc attgtgctgg
     2041 ccataggctt gatccacgcc atcggtctgt ggaccggctg gcggacactg taagcgaaac
     2101 aaacaagacc ctcgctaaag cagcgagtga tcgcgtcgta acggcagcgt ccccatacaa
     2161 atccttttcg ctcaagcagc gaggctccgc gagccgagcg ttagcgaccc taaagaaaaa
     2221 ggcgctgcgt ctttcgacac agcgcctttt tgaaatggtg cccaggagag gactcgaacc
     2281 tccacgacct ttcgatcact ggcacctgaa gccagcgcgt ctaccgttcc gccacctggg
     2341 ctccggacgg gcgctgttga aggcgttacc gtctggaagg cgcggacctc tacgggaggc
     2401 gggggagcgg gtcaatatgc atttacgaca agatgcatac atcgcgttgc gacgcggcgg
     2461 tgcatcgtct atcccgcccc tcacaggatt ttatcggagt ttcccatgtc cgcgttcagc
     2521 ccgggtctgg tcaccgtgtt cggaggttcc ggcttcgtcg gcgcacaggc cgttcgcgcc
     2581 ctggccaagg ccggctggcg cgtccgcgtc gcggtacgct cgcccaatac ctcctacgat
     2641 ctccgccctc tgggcgacgt cggccagatc cagccggtgc gctgcgacgt cacctcggcg
     2701 gccgacgtgg cggcggcgct gaagggcgcg aatgccgcgg tcaatctggt cgggatcctg
     2761 tacgagtccg gcaagcgttc gttcgaggtc atgcacgtcg acggcgcccg cacgatcgcc
     2821 gaagcttgcg c
//
LOCUS       DASVTM010000391         5165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_142346,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000391 DASVTM010000000
VERSION     DASVTM010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142346"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VF633_10745"
     CDS             complement(<1..921)
                     /locus_tag="VF633_10745"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8471581.1"
                     /translation="MKTAGVHRFEALDSLRGVCAILVLMFHMPVASHWRDWPLIQHGY
                     LFVDYFFVLSGFVIAHAYAQRLKTRRDAGRFMVRRLGRVWPLHVLMLVCFIGLELVRL
                     WTHFDLAPPFTRDRSIDTIFTNLLLIQAWHIHPYLTWNGPSWTLSAEVACYLIFAGLV
                     LVAPQRFRWIGAVLAVIGAVIVVMFARRWMNTTFDFAVPRAVYGFFLGCLLQGFWTRI
                     PRLKAAGPAKNWGATALEVASILACCLFIAWAEGPVTVLVSVVFLGIVWVFAGEDGLL
                     SRALDHPALVTLGRWSFAIYMVHMFVLTVMM"
     gene            complement(1036..1458)
                     /locus_tag="VF633_10750"
     CDS             complement(1036..1458)
                     /locus_tag="VF633_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8471582.1"
                     /translation="MLTAAEVIAMLDLAPHPEGGHYRETFRDPREVDGRSVGTAIYFL
                     LAEGESSHWHQVDAAELWHFYEGAPLELEIAANGMRRTVTLGADLSAGQRPQGVVPTG
                     AWQAARTLGAWTLVGCTVAPGFDFSGFEMAPAGWSPST"
     gene            complement(1502..2917)
                     /locus_tag="VF633_10755"
     CDS             complement(1502..2917)
                     /locus_tag="VF633_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX8471583.1"
                     /translation="MNASIAALAYLVAGVLFILSLRGLSSPETSRQGNTMGMVGMAIA
                     VAVTLLTLGFSGALDPLTLALLAGGVIIGGGGGALIAAKVKMTDMPQLVAAFHSLVGM
                     AACLVAVGAVYAPEAFGISDGLGGIKLQSIIELALGVAIGAITFTGSVIAFAKLNGNM
                     SGAPIILPARHLINIGLALTLGLMIGIMVASGGTATWAFWGIFAIALIMGATLIIPIG
                     GADMPVVVSMLNSYSGWAAAALGFTLENIALIITGALVGSSGAILSYIMCKGMNRSFI
                     SVILGGFGGDSGPTGEARVETRPVKQGSAEDAAFIMKNASKVIIVPGYGMAVAQAQHA
                     LREMADKLKEEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINAEFATAD
                     VAFVIGANDVTNPAAKTDPQSPIYGMPILDVEKAGTVLFIKRGMAAGYAGVENELFFR
                     DNTMMLFSDAKKMVEGIVKSL"
     gene            complement(2967..3365)
                     /locus_tag="VF633_10760"
     CDS             complement(2967..3365)
                     /locus_tag="VF633_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471584.1"
                     /translation="MKALLILAAALSIVSATAASAQALSLKTDVPGPPFAGARWAVAA
                     PADTRWTLACRFAPITYQADRYDLHRWSNGLTREGRGSDQGRLPGPDGRCILTLTSGP
                     GPIGVGLARKGATTVADGTNDPARPARASF"
     gene            complement(3362..3652)
                     /locus_tag="VF633_10765"
     CDS             complement(3362..3652)
                     /locus_tag="VF633_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471585.1"
                     /translation="MPIDPAALARIVSWIATGLGVSLWVWSWVREQNPIQKQRFLDCG
                     VVLVFAATLVRVVVQERAMTPVDWALTFLSPLFIAAALWRLARTACPTDLDR"
     gene            complement(3652..3993)
                     /locus_tag="VF633_10770"
     CDS             complement(3652..3993)
                     /locus_tag="VF633_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX8471586.1"
                     /translation="MEHVDPTVFRLAIFVLAIFVGYYVVWSVTPALHTPLMAVTNAIS
                     SVIVVGGLIAAAATAGDTGATPWIAKGAGVAAVTLASVNIFGGFMVTRRMLAMYKKKE
                     RPAKAVEASKA"
     gene            complement(3993..5126)
                     /locus_tag="VF633_10775"
     CDS             complement(3993..5126)
                     /locus_tag="VF633_10775"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX8471587.1"
                     /translation="MAVAIAVTRERREGETRCAITPETVKKFIGLGATVTVETGTGAG
                     SSFPDSEYEAAGASVKPDTRAVLDGADIVLAVRGPTAQEISAIKPGAVVVALLDAYRE
                     KDTVAALAGAGTTAFAMEFVPRISRAQVMDALSSQANLAGYRAVIEAAHAYGRGFPMM
                     MTAAGTVAPAKIFVMGVGVAGLQAIATARRMGAVVTATDVRPATKEQVESLGAKFTAV
                     EDEEFKNAQTAGGYAKPMSPEYQAKQAILTGEHIKKQDIVITTALIPGRAAPVLVTAD
                     QVATMKPGSVLIDLAVEAGGNVEGARVGETVVTPNGASIVGIANLPGRIASDASSLYA
                     KNLFAFVGLMLKDGALSVDLEDEILKAAVVTHGGAVVHEGVKG"
BASE COUNT          945 a         1640 c         1774 g          806 t
ORIGIN      
        1 catcatcacc gtcaggacga acatatggac catgtagatc gcgaacgacc agcgtccaag
       61 ggtgaccagc gctggatggt cgagcgcgcg tgacaggagt ccgtcctcac ccgcaaagac
      121 ccagacgatg cctaggaata cgacgctgac cagaacggtg accggtcctt cggcccaggc
      181 gatgaacagg cagcatgcca ggattgaggc gacttccaac gcggtggcgc cccagttctt
      241 cgctggaccc gctgccttaa ggcgtggaat tcgcgtccag aaaccctgca gcaggcagcc
      301 gagaaagaag ccgtagacgg cgcgtgggac ggcgaagtcg aacgtggtgt tcatccagcg
      361 gcgcgcgaac atcacgacga tgacggcgcc gatgaccgcc agcacggcgc cgatccagcg
      421 gaaccgctgc ggcgcgacca gaaccagacc cgcgaagatc agatagcagg ccacctcggc
      481 gctgagcgtc cacgaggggc cgttccaggt cagataggga tgaatgtgcc aggcctggat
      541 cagcagcagg ttggtaaaga tggtgtcgat cgaccgatcg cgggtgaagg gcggcgcgag
      601 atcgaaatgc gtccacagcc ggaccagctc aagcccgatg aagcagacca gcatcaggac
      661 gtgcagcggc cagacccggc ccaaccggcg caccatgaac cgccctgcgt cgcgccgcgt
      721 cttcagccgc tgggcatagg catgggcaat gacgaagcct gacaggacga agaagtaatc
      781 gacgaacaga tagccgtgct ggatcagcgg ccagtcgcgc caatggctgg cgaccggcat
      841 gtggaacatg agcacgagga tggcgcaaac cccgcgaagg gagtccagag cctcaaaccg
      901 atgtacgccc gccgttttca cagcggcgtg ataccgggtc cgcaagcgct gcgatagtgg
      961 cgtcgttcag ccgacacacc gcctcggccc gcccggactg tcgagcatga caggcaagcc
     1021 tcttgcgtag gaaatctatg tggacggtga ccaacccgcc ggggccatct cgaagcccga
     1081 gaagtcgaaa ccgggcgcga ccgtgcaccc caccagcgtc caggcgccca gcgtccgcgc
     1141 cgcctgccat gccccggtcg gcacgacgcc ctggggcctt tgtccggcgg aaagatcagc
     1201 gccgagagtc actgtgcgcc gcataccgtt ggcggcgatc tcaagctcca gcggcgcgcc
     1261 ctcatagaaa tgccagagct cggcggcgtc cacctgatgc cagtgcgagc tctcgccctc
     1321 tgccagtagg aaataaatcg ccgtccccac cgaccgccca tcgacctcgc gcggatcccg
     1381 aaacgtctcg cgataatgcc cgccctccgg atgcggagcg agatcgagca tggcgatgac
     1441 ttcggcggca gtgagcatgg actcacctta gggcgattcc ggctgatcgt tcaggctgcg
     1501 atcaaagcga cttcacgatc ccctcgacca tcttcttcgc atccgagaac agcatcatgg
     1561 tgttgtcgcg gaagaacagt tcgttctcga cgccggcgta gcctgccgcc atgccgcgct
     1621 tgatgaacag gacggtgccg gccttttcga cgtcgaggat gggcatgccg tagatcggcg
     1681 actgggggtc ggtcttggcg gccgggttgg tgacgtcgtt ggcgccgatg acgaaggcga
     1741 cgtcggcggt ggcgaactcg gcgttgatgt cctccagctc gaacacctcg tcatagggga
     1801 cgttagcctc ggccagcagg acgttcatgt ggcccggcat ccggcccgcg acggggtgga
     1861 tggcgtattt cacctcgacg ccctcttcct tgagcttgtc ggccatctcg cgcagggcgt
     1921 gctgggcctg ggcgacggcc atgccgtaac cggggacgat aatgaccttg gaggcgttct
     1981 tcatgatgaa ggctgcgtcc tcggctgagc cctgcttgac cggacgggtc tcgacgcgcg
     2041 cctcgcctgt ggggcctgag tcgccgccga agccaccgag gatgacgctg atgaagctgc
     2101 ggttcatgcc cttgcacatg atgtagctga ggatcgcgcc tgacgaaccg accagcgcgc
     2161 cggtgatgat cagggcgata ttctcgagcg tgaagccgag cgcggcggcg gcccagccgg
     2221 aataggagtt cagcatcgac accaccaccg gcatgtcggc gccgccgatg gggatgatca
     2281 gtgtcgcacc catgatcagg gcgatggcga agatgcccca gaaggcccag gtggcggtgc
     2341 cgcccgaggc gaccatgatg ccgatcataa ggcccagcgt cagcgcgagg ccgatgttga
     2401 tcaggtggcg ggcaggcagg atgatcggag cgccgctcat attgccgttc agcttggcga
     2461 aggcgatgac cgagccagtg aaggtgatgg cgccgatggc gacgcccagc gccagctcta
     2521 tgatcgactg cagcttgatg ccgcccagac catccgagat gccgaacgcc tcaggcgcat
     2581 agacggcgcc gacggcgacc agacaggcgg ccatgccgac gaggctgtgg aaggcggcga
     2641 ccagttgcgg catgtcggtc atcttgacct tcgccgcgat cagagccccg ccgccgccgc
     2701 cgatgatgac gccgcccgcg agcagcgcca gcgtcagcgg atccagcgcg ccggagaagc
     2761 cgagcgtcag cagggtcacg gcgacggcga tggccatccc gaccatgccc atcgtattgc
     2821 cctgacggct ggtctccggg ctcgacaggc cgcgcaggct caagatgaaa agaacgcctg
     2881 cgaccagata ggccagcgcc gctatggatg cgttcatcgc gtccccccga agtgtcttgt
     2941 tggtctggtc gtgtgagttt gagccatcaa aagctggctc gcgccggccg ggccggatcg
     3001 ttagtgccgt cggcgacggt ggtcgcgcct ttacgggcca gaccgacgcc gatcgggccg
     3061 ggtccggagg tcagggtcag aatgcagcga ccgtcagggc cgggcaggcg accttggtcg
     3121 gagccgcgtc cctctcgggt gaggccgttg gaccagcggt gcaggtcgta tcggtcggcc
     3181 tgataggtga tgggggcgaa gcggcaggcc agcgtccagc gcgtgtcggc gggtgcggcg
     3241 acggcccagc gggcgccggc gaacggaggg cccgggacgt ctgtcttcag gctcaaggcc
     3301 tgcgccgagg cggcggtggc gctgacaatg gacagggcgg cggcgaggat cagcaacgcc
     3361 ttcatcggtc gagatccgtc ggacaggctg tgcgcgccag tcgccaaagg gcggcggcga
     3421 tgaacagggg cgacaggaag gtcagggccc agtcgaccgg cgtcatcgcc cgctcctgaa
     3481 ccacgacccg cacaagcgtc gcggcgaaga caagcactac gccgcagtcg aggaagcgct
     3541 gcttctggat cgggttctgc tcacgcaccc agctccacac ccacaggctg acgccgagcc
     3601 ccgtggcgat ccacgagacg atacgggcca aggctgcagg gtcgatgggc atcaagcctt
     3661 cgatgcctcg acagccttcg cggggcgttc cttcttcttg tacatggcca gcatgcgtcg
     3721 ggtgaccatg aagccgccga agatgttgac gctggccagg gtcacggcgg cgacgccggc
     3781 gcccttggcg atccagggcg tggctccggt gtcaccggcg gtggccgctg cggcaatcag
     3841 gccgccgacg acgatgacgc tggagatggc gttggtcacg gccatcagcg gcgtgtgcag
     3901 ggcaggggtc accgaccaga cgacatagta gccgacgaag atcgccagca cgaaaatggc
     3961 cagacgaaag acggtgggat cgacgtgttc catcagcctt taactccttc atgaaccaca
     4021 gccccgccgt gggtgacgac cgccgccttg aggatttcat cctcgagatc aacgcttagc
     4081 gcgccgtcct tcagcatcag tccgacgaat gcgaacaggt tcttggcgta gagagacgag
     4141 gcgtcggagg cgatgcggcc gggcaggttg gcgatgccga cgatggaggc gccgttgggg
     4201 gtgaccacgg tctcgccgac cctggcgccc tcgacattgc cgccggcttc gacggccaga
     4261 tcgatcagga ccgagccggg cttcatcgtc gcgacctgat cggcggtgac cagaaccggc
     4321 gccgcgcggc ccgggatcag ggccgtggtg atgacgatgt cctgcttctt gatatgctcg
     4381 ccggtcagga tggcctgttt ggcctggtac tcgggcgaca taggcttggc gtagccgccg
     4441 gcggtctggg cgttcttgaa ctcctcgtcc tcgacggcgg tgaacttggc gccgaggctt
     4501 tcgacctgtt ccttggtggc ggggcggacg tcggtggcgg tgacgaccgc gcccatgcgc
     4561 cgcgccgtgg cgatggcctg aagcccggcg acgccgacgc ccatgacgaa gatcttggcc
     4621 ggggcgacgg tgcccgccgc cgtcatcatc atcgggaagc cgcgaccata ggcgtgggcc
     4681 gcctcgatta cggcgcgata gccggcgaga ttggcctgcg acgacagggc gtccatgacc
     4741 tgtgcgcgcg aaatgcgggg cacgaactcc atggcgaagg cggtcgtacc ggcgcctgca
     4801 agggccgcga cggtgtcctt ctcgcggtag gcgtccagca gggcgacgac gaccgcgccg
     4861 ggcttgatgg cgctgatttc ctgcgccgtc ggaccgcgca ccgcgagcac gatgtcggcg
     4921 ccgtccagca ccgcacgcgt gtcgggcttc accgaagcgc cggcagcctc gtactcggaa
     4981 tcggggaagg atgacccggc gccggtcccc gtctcgacgg tgacggtggc gccgagaccg
     5041 atgaatttct tcacggtctc gggcgtgatg gcacagcgcg tttcgccttc acggcgttcg
     5101 cgggtcacgg cgatggcgac tgccaagcga atctccccaa gtctcgttct tggaaatcac
     5161 gttag
//
LOCUS       DASVTM010000392         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_731296,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000392 DASVTM010000000
VERSION     DASVTM010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731296"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF633_10780"
     CDS             complement(<1..405)
                     /locus_tag="VF633_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX8471588.1"
                     /translation="MTVSTLNPSTNSTPLKQVWPADMNPAEMTPEQMVLTALRHEIDL
                     IDDQILELFERRLAIAASVGKAKDAPHGPHTKLRPDREQTVLGRVLSQCQPENVEAVS
                     GLWREIVGWGLARQGTLQVQVWAPNDPTRAFDG"
     gene            complement(691..1269)
                     /locus_tag="VF633_10785"
     CDS             complement(691..1269)
                     /locus_tag="VF633_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEX8471589.1"
                     /translation="MTDLSSPEDAANSRVAFQGAPGAFSHEACVALRPWDEAVAYETF
                     AGALDALKSGECNMALIPVENSTIGVVEPAATLVRESGFEVLADVWRPIRHALMALPG
                     TRLADIRTIESHPIALDQCRETLSQLNARIVIGFDTAGAARDVAEAGDRTRAALAPMA
                     AAEVYGLTILRNDLQDSADNRTRFVLLGAQKG"
     gene            complement(1283..2653)
                     /locus_tag="VF633_10790"
     CDS             complement(1283..2653)
                     /locus_tag="VF633_10790"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX8471590.1"
                     /translation="MNTRWTPESWRSKPVVHMPMDYPDARALTAVEDELRAMPPLVFA
                     GEARTLTSKLAQVEKGEAFLLQGGDCAESFKEFSTDNIRDTFRLILQMAVVLTFAGRK
                     PVVKVGRIAGQFAKPRSSAVEEIGGVELPSYRGDNINGSGFTPEERIPDPQRLLKAYN
                     QSAVTLNLLRAYANGGYADLYNIHRWTLGFADNSAGAMYGELADKITESLAFMEAIGV
                     TPQTHPDLARVEVFTSHEALLLNVESALTRLDGNTGEWFDTSAHMLWIGERTRQLDGA
                     HVEFMRGIKNPIGVKCGPTMEPDDLLPLLDKLNPENISGRMTIIGRFGHDKVGKRLPH
                     LMAAVKAAGHKVIWSIDPMHGNTLKADNGYKTRPFDRIMTEVRTFIDVAEAEGVHPGG
                     VHLEMTGQNVTECIGGATAVTEDDLSSRYHTHCDPRLNADQALELAFLVAERLKVGRL
                     SQSRAA"
     gene            2730..2954
                     /locus_tag="VF633_10795"
     CDS             2730..2954
                     /locus_tag="VF633_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX8471591.1"
                     /translation="MDPLLSRITVEADKNFGKPSIRRMRIRVQDVLEMLAAGDTPEDI
                     LEGFPYLEPDDIRACIAYAAAVVSKEIAVA"
     gene            2960..3277
                     /locus_tag="VF633_10800"
     CDS             2960..3277
                     /locus_tag="VF633_10800"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX8471592.1"
                     /translation="MIDANLPVEVVDWLNSIGHDSVHVSMLGIENNDGLIWSYAIGTG
                     SVVVTKDSDFADWVRERSPAPRLVWLRTGNLRKKQQLEHLARGWLQVLTRLTEGEQVV
                     EVR"
     gene            complement(3319..4710)
                     /gene="gor"
                     /locus_tag="VF633_10805"
     CDS             complement(3319..4710)
                     /gene="gor"
                     /locus_tag="VF633_10805"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX8471593.1"
                     /translation="MADYDYDLFVIGAGSGGVRAARLTALGGKRVAIAEEFRVGGTCV
                     IQGCVPKKFMVMASDFAHQFHTAEGYGWDVQATFDWPKFLQAKDVEIARLSGIYAANL
                     GKAGVDLVNGRAVFVDAHTVKVTKRDGTDGGTFTAEKILIATGGRPWVPENLTGIEHA
                     ITSEQAFHLPELPKRILIAGGGYIAVEFAGIFAGLGVDTTLVYRGPNILRGFDDDVRA
                     HLAGEIEKRGIKVILGCQHSSLEKTETGIVNHLENGMTIETDVVMFATGRIPYVKDLG
                     LEKAGVTLSDKGAIVVDELSKTSADNIWAIGDVTDRMNLTPVAIREAVAFHETVYKNN
                     PQHFDYEAVATAVFSQPPVGVVGLSESEARHICKAGVDVYVARFRPMKYAFTGSDERV
                     LMKLVVDADSQRVVGVHIVGPEAPEMIQLAAIAVKAGLTKAQWDATCAVHPTMAEELV
                     TLKDKQTPSNTAG"
     gene            complement(4737..>5184)
                     /gene="rpiA"
                     /locus_tag="VF633_10810"
     CDS             complement(4737..>5184)
                     /gene="rpiA"
                     /locus_tag="VF633_10810"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEX8471594.1"
                     /translation="DGADEIGPGLALTKGGGAALLREKLVWEASKSCIVIADAAKVVP
                     VLGQFALPVEVVAFGHKTTANRIADVLLDHEIIMPARARTADRGLVRTDGGNLIYDAK
                     CVAIHDPVRLADDLKLITGVVEHGLFLDLADEAIIGGDDGVSTLFP"
BASE COUNT          874 a         1683 c         1688 g          939 t
ORIGIN      
        1 gccgtcgaag gctcgggtcg ggtcattggg cgcccagacc tgcacctgca gcgtaccttg
       61 gcgggccagt ccccagccga cgatctcgcg ccagagacca gagacggcct cgacgttctc
      121 gggctggcac tgggacagga cgcggcccag cacggtctgt tcgcggtcgg gacggagctt
      181 ggtgtggggg ccgtgcgggg cgtccttggc cttgccgacg ctggcggcga tggcgagacg
      241 gcgctcgaac agctcgagga tctggtcgtc gatcaggtcg atctcgtgcc gcagggcggt
      301 caggaccatc tgttcgggcg tcatctcggc agggttcatg tcagcgggcc agacctgctt
      361 gaggggtgtg gaattggtgg acggattcag ggtggagacg gtcatttcag aagggctttc
      421 ggaatacgga atttcgatga agggtgcgaa tgggcgcgcc gccgctcggg tcaggagcgg
      481 tcgccggcga ggctgtcagg agcgaggtgc gctcgaggcc tcagccggcg tatcgaaagc
      541 cgtaatagga gccgctaaaa taaaagccgg caaccgcgcg cgaacgggtc gtaagggtca
      601 gtacagaggc gcgcagcggg gacatattgg gtccagttcg cggaccggga gggtccgtag
      661 ggccttaaga cacgcaacga tcgtgcgccg tcaacccttt tgagcgccca gcagcacgaa
      721 acgcgtccgg ttatcagcgg aatcctgcag gtcgttgcgc agaatcgtca gcccatagac
      781 ctccgccgcc gccatcggcg ccaaggctgc gcgggtccgg tctcccgcct ccgccacgtc
      841 ccgggccgca ccggcggtgt cgaagccgat gacgatcctg gcgttcaact gcgacaaggt
      901 ctcgcggcac tggtccagcg cgatggggtg gctttcgatg gtgcggatat cggccaatcg
      961 cgtgcccgga agcgccatca gggcgtgccg aatgggacgc cagacatcgg ccagcacctc
     1021 gaaccccgac tcgcgcacca gcgtcgccgc cggctccacg acgcctatcg tcgagttctc
     1081 gaccgggatc agcgccatgt tgcactcgcc gctcttcagc gcatcgagcg ctccggcgaa
     1141 ggtctcgtag gccaccgcct cgtcccaagg gcgcagggcg acgcaggcct cgtggctgaa
     1201 cgcgcccggc gcgccttgaa aggccacgcg ggaattggcc gcgtcttcgg gactggaaag
     1261 gtcggtcacc gggccgaccc aatcacgccg cgcgcgactg actgaggcgc ccgaccttga
     1321 gccgctcggc cacaaggaag gccagctcca gcgcctgatc ggcgttcagg cgcgggtcgc
     1381 agtgggtgtg gtagcggctc gacagatcgt cctcggtcac ggccgtggcc ccgccgatgc
     1441 attcggtcac gttctggccg gtcatctcca gatggacgcc gccggggtgg acaccctcgg
     1501 cctcggccac gtcgatgaag gtgcggacct cggtcatgat ccggtcgaac ggccgggtct
     1561 tgtagccgtt gtcggccttc agcgtgttgc cgtgcatcgg gtctatcgac cagatcacct
     1621 tgtgaccggc cgcctttacc gccgccatca ggtgcggcag gcgcttgccg accttgtcgt
     1681 gaccgaagcg accgatgatg gtcatccggc ccgagatgtt ctcgggattc agcttgtcca
     1741 gcagcggcag caggtcgtcc ggctccatgg tcgggccgca cttcacgccg atcgggttct
     1801 tgatcccgcg catgaactcg acgtgagcgc cgtccagctg acgcgtgcgc tcgccgatcc
     1861 acagcatgtg agccgaggta tcgaaccact cgccggtgtt cccgtccaga cgggtcagcg
     1921 cgctctcgac gttcagcagc agggcctcgt gcgaggtgaa gacctcgacg cgggcgagat
     1981 ccggatgcgt ctgcggcgtg acgccgatcg cttccatgaa cgccaggctt tcggtgatct
     2041 tgtcggccag ttcgccgtac atcgcgcccg cgctgttgtc agcgaaaccg agcgtccagc
     2101 gatggatgtt atacagatcc gcatagccgc cgttggcgta ggcgcgcagc aggttcagcg
     2161 tcaccgccga ttggttgtaa gccttcagca ggcgctgtgg atccgggatc cgctcttccg
     2221 gcgtgaagcc cgagccgttg atgttgtcgc cgcgatagga cggcaactcg acgccgccga
     2281 tctcctcgac cgccgacgag cgcggcttgg cgaactgacc ggcgatgcgg ccgaccttca
     2341 ccaccggctt gcgacccgca aacgtcagca cgaccgccat ctgcaggatc aggcggaagg
     2401 tgtcgcggat gttgtcggtc gagaattcct tgaagctctc ggcgcaatcg ccgccctgca
     2461 gcaggaaggc ctcgcccttt tcgacctggg cgagcttgga ggtgagcgtc cgcgcctcgc
     2521 ctgcaaacac gagcggcggc atggcgcgca gttcgtcttc gacggcggtc agggcgcgcg
     2581 cgtcaggata gtccatcggc atatgcacga cgggcttcga tctccagctc tctggggtcc
     2641 agcgggtatt catgccgcgc tacatagtcc gaagggcccg attggacaat gaatgtgcgc
     2701 gttgtttcgg tctgtggtaa gttcaagcca tggacccgct gctttcacgc atcactgtcg
     2761 aggccgacaa gaacttcggc aagccaagca ttcgccggat gcgcattcgt gtgcaggacg
     2821 ttttggaaat gctggccgcc ggggacacgc cggaagatat ccttgagggc tttccctatc
     2881 tggagcctga cgatatccgc gcctgcatcg cctatgctgc tgcagtggtc agcaaagaaa
     2941 tcgctgtcgc ttgaaattcg tgatcgacgc caatctcccg gtggaggtgg tggactggct
     3001 aaactccatc ggtcacgaca gtgttcacgt gtcgatgttg ggtatcgaga acaatgacgg
     3061 cttgatctgg agttacgcta tcgggacggg ctccgtggtc gtaaccaaag actcggactt
     3121 cgcggattgg gtccgcgaac gctctcctgc tcccaggctc gtctggctac gcacgggaaa
     3181 cctgcgaaag aaacaacagc tagaacatct cgcccgcggc tggcttcagg tgctgacgcg
     3241 cctgaccgaa ggcgagcagg ttgtggaggt gcgctaaact ggttcgggcc acagtccgaa
     3301 tcgagcgcac cccgccccct accccgccgt gttcgacggt gtctgcttgt ccttcagggt
     3361 gaccagttcc tcggccatgg tcgggtggac ggcgcaggtg gcgtcccatt gggccttggt
     3421 cagtccggcc ttcaccgcga tggcggccag ctggatcatc tccggcgcct cgggtccgac
     3481 gatgtgtacg cccacgacgc gctggctgtc ggcatcgacg accagcttca tcagcacccg
     3541 ctcatccgat ccggtgaagg cgtatttcat cggccggaac cgggcgacat agacatccac
     3601 gcccgccttg cagatgtgcc gcgcctcgct ctcggacagg ccgaccacgc cgactggcgg
     3661 ctgcgagaac accgccgtcg ccacggcctc atagtcgaaa tgctgcgggt tgttcttgta
     3721 gacggtctcg tggaaggcga ccgcctcgcg gatcgccacc ggcgtcaggt tcatccggtc
     3781 ggtcacgtcg ccgatggccc agatgttgtc ggccgaggtc ttcgatagct cgtcgaccac
     3841 gatcgcgccc ttgtcgctga gcgtgacgcc ggccttctcc agccccagat ccttgacgta
     3901 gggaatgcga ccggtggcga acatcacgac gtcggtctcg atcgtcatcc cgttttcgag
     3961 atgattgacg atgccggtct cggtcttctc gaggctcgag tgctggcaac cgaggatgac
     4021 cttgatgccc cgcttctcaa tttcaccggc cagatgcgcg cgcacgtcgt catcgaaacc
     4081 gcgcaggatg ttcggcccgc gatagaccag cgtcgtatcg acgccgagcc cagcgaagat
     4141 gcccgcgaac tccaccgcga tatagccgcc gcccgcgatc aggatgcgct tgggcagttc
     4201 cggaaggtgg aaggcctgtt cggaggtgat ggcgtgctcg atgcccgtca ggttctccgg
     4261 cacccaggga cgaccgcccg tggcgatcag gatcttctcg gcggtgaacg tcccgccgtc
     4321 cgtgccgtcg cgcttggtga ccttcaccgt atgggcgtcg acaaagaccg cccggccatt
     4381 gaccagatca acgcccgcct tgccgagatt ggctgcatag atgcccgaca ggcgcgcgat
     4441 ctcgacgtcc ttggcctgca gaaatttggg ccagtcgaac gtcgcctgga catcccagcc
     4501 atagccctca gccgtgtgga actggtgggc gaagtccgac gccataacca tgaacttctt
     4561 gggcacgcag ccctggatca cgcaggtgcc gccgacccgg aactcttcgg cgatggccac
     4621 ccgcttgccg cccagcgccg tcagccgcgc cgcacgcacc ccacccgagc ccgcgccgat
     4681 gacaaagagg tcgtagtcgt aatcggccat gagtttcttt cagctgagat gatcggtcac
     4741 ggaaataggg tgctgacccc gtcatcgcca ccaatgatcg cttcgtcggc gagatcgagg
     4801 aacaggccgt gctcgaccac gccggtgatc agcttcaggt cgtcggccag ccgcaccgga
     4861 tcatggatgg cgacgcattt ggcgtcatag atcaggttgc cgccgtcggt gcggaccagc
     4921 ccccggtcgg cagttcgcgc gcgcgccggc atgatgatct cgtggtccag cagcacgtcg
     4981 gcgatacggt tggcggtcgt cttgtgcccg aaggcgacga cctcgaccgg cagggcgaac
     5041 tgccccagca ccggcacgac cttggccgcg tcggcgatga cgatgcagct cttcgacgcc
     5101 tcccacacca gcttctcgcg cagcaaagcc gccccgccgc ccttggtcag cgccagcccg
     5161 ggaccgatct cgtcggcgcc gtcc
//
LOCUS       DASVTM010000393         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_462536,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000393 DASVTM010000000
VERSION     DASVTM010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462536"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..>2000
                     /locus_tag="VF633_10815"
     CDS             81..>2000
                     /locus_tag="VF633_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8471595.1"
                     /translation="MTRSTEATMTNRFELLVQSVTDYAIYMLDPSGIVTSWNAGAERF
                     KGYAADEIIGRHFSTFYTPEDLQQEIPTKALATARQEGRFEAEGWRLRKDGSRFWANV
                     VIDPVRDTSGELIGFAKVTRDLTTRRQAEEELRRSEERFRFLLNSVTDYAIYMLDRDG
                     YVTSWNGGAERFKGYTADEIIGEHFSRFYAPEDRASDLPANALKTALNEGRFEAEGWR
                     YRKDGTNFWANVVIDPVHNDQGELIGYAKITRDLTERRRSQLALEQAQQAFFQSQKME
                     AIGKLTGGVAHDFNNLLAAIMGSVDLARGRLAKGADITRFLDNIMQAAERGATLTHRM
                     LAFARKQDLALQTVELPDLVRGSADLLQRTIGPGVTIDTRFPLLLKPVRADPAQLDLA
                     LINLAVNARDAMPDGGTIILAARHEQVRTRNGLEPGQYVCLSVTDEGEGMDEDTRSRA
                     MDPFFTTKGVGSGTGLGLSMVAGMAEQCGGKLVLKSKPGEGTTAEIWLPVAIRVKPAT
                     EEVSAAEGPLRFLQPLTVLAVDDDELVLINTAALLGDMGHTVLQAASGQEALRILGNH
                     RIDLVITDYAMPGMTGGELIRAIEGGWKTLPILMVSGYAEIPEGEALGVPRLAKPFRP
                     HQLAAAVAAAVGGAQG"
BASE COUNT          395 a          640 c          624 g          341 t
ORIGIN      
        1 tgggcagggg cgaggaccgt cgacgccctg gaacctaacg gaccccttgg cattcgtttc
       61 aggttatggc gggggcctca atgactcgat cgactgaagc gaccatgacc aaccggttcg
      121 aactgctcgt tcagagcgta accgactacg ccatctacat gcttgacccc tcaggcatcg
      181 tcacgagctg gaacgccggc gccgaacggt tcaagggcta cgctgcggac gagatcatcg
      241 ggcggcattt ctcgacattc tacacccccg aggatctcca gcaggaaatt ccgaccaagg
      301 cgctggccac ggcaaggcag gaaggccggt tcgaggccga gggctggcgg cttcgcaagg
      361 acggctccag gttctgggcc aacgtggtca tcgatccggt ccgggacacc tccggtgagc
      421 tgatcgggtt cgccaaggtc acgcgcgatc tgactacgcg cagacaggcc gaagaggagc
      481 ttcgtcgaag cgaggaacgc ttcaggttcc tgctcaacag tgtcaccgac tatgcgatct
      541 atatgctcga tcgcgacggc tatgtgacca gctggaacgg cggcgccgag cgctttaaag
      601 gctatactgc cgatgaaatc atcggcgaac atttctcccg cttctacgcg cccgaagacc
      661 gggcctcgga cctcccggcc aacgctctga aaacagcgct gaacgaggga cgtttcgaag
      721 ccgagggctg gcgctatcgc aaagacggca caaatttctg ggccaacgtg gtcatcgatc
      781 ctgtgcataa cgatcaaggc gagctcatcg gctacgccaa gatcacccgc gatctgacgg
      841 agcgtcggcg atcccagctg gctttggaac aggcgcagca ggcctttttc cagtctcaga
      901 agatggaagc catcggcaag ctcaccggcg gcgtggccca cgatttcaac aacctcctcg
      961 ccgccatcat gggcagcgtt gacctggcgc ggggccggct cgcaaagggc gcggacatta
     1021 cccggttcct cgacaacatc atgcaggcgg cggagcgggg cgccacactg acccatcgca
     1081 tgctcgcctt cgcccgcaag caggatctgg cgcttcagac cgtcgaactg cctgatcttg
     1141 ttcgtggctc ggcggatctc ctgcagcgga cgatcggacc gggcgtcacc atcgacaccc
     1201 ggttcccgct tctcctcaaa cccgttcggg ccgacccagc gcagcttgac ctcgccctga
     1261 taaacctggc ggtgaacgcg cgcgacgcca tgccggacgg gggaacgatc attctcgccg
     1321 cccgccacga gcaggtcagg actcgcaacg gtctcgagcc gggacaatac gtctgccttt
     1381 ccgtcacaga cgagggtgaa gggatggacg aggacacgcg gtccagggcg atggatccgt
     1441 tcttcaccac caagggcgtc ggcagcggca ctggtctggg attgtcgatg gtcgccggga
     1501 tggccgaaca atgcggcgga aagcttgttc tgaaaagcaa gccgggcgaa ggaacgactg
     1561 ccgaaatctg gctacccgtc gccattcgcg tgaagcccgc gacagaggag gtttccgccg
     1621 ccgagggacc tctgcgcttt cttcagccac tcaccgttct ggcggtggat gatgacgaac
     1681 tggttctgat caacacagcc gcgttgcttg gcgacatggg gcacaccgtg cttcaggccg
     1741 cgtccgggca agaggcgctg cgcatactgg gcaaccatcg catcgatctc gtcatcaccg
     1801 actacgccat gccggggatg accggcggcg agcttatccg ggcgatcgaa ggagggtgga
     1861 agacgctgcc gatcctcatg gtctccggct atgccgaaat ccccgagggc gaagccctgg
     1921 gcgttccgcg tctcgccaaa cccttccgtc cgcaccagct cgccgcagcc gtcgccgcag
     1981 cggtgggagg cgctcagggc
//
LOCUS       DASVTM010000394         4388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_38519, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000394 DASVTM010000000
VERSION     DASVTM010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38519"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF633_10820"
     CDS             complement(<1..218)
                     /locus_tag="VF633_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX8471596.1"
                     /translation="MAKRTISDIEKIWSNVEGVKKLSDRAVGIGPFGIGLDGLLAFVP
                     GVGLAYTVGAAGWLLMQAARAKASPSTLA"
     gene            complement(329..1540)
                     /locus_tag="VF633_10825"
     CDS             complement(329..1540)
                     /locus_tag="VF633_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX8471597.1"
                     /translation="MTASVADEPWLKSPATRAVLTALQAEGGPDCVRFVGGCVRNTLM
                     GQKVDDLDLATRLLPEVAMGALKKAGLKVVPTGLAHGTITAVSSGRPYEITTLRRDVE
                     TDGRKAVVAFTDDWAEDAGRRDFRLNALYADAEGQVFDPTGHGVNDAREGQIVFVGDP
                     ERRIREDYLRILRFFRFFAWYGRGEPDAAGLKACADLSEGLVRLSAERVSKELLKLLA
                     ARDPRPTVQAMASAGVLHAILPEAGPLAVFETMVDLSPDPVLRLSALLSPEMAQVEKA
                     ARRLRLANASRDRLIAAVGGEIDTTMDDRAARALIYRLGRPTFIDRVIRAQARSGGPG
                     QGSNLIALAEAWPIPRLPVGGRQVAAAGLSAGPMTGRVLTAFEDSWIADDFPDHGHAG
                     RLEALVETLQA"
     gene            complement(1537..2199)
                     /locus_tag="VF633_10830"
     CDS             complement(1537..2199)
                     /locus_tag="VF633_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX8471598.1"
                     /translation="MSLSTLKARLVERLHSPDSWRPDADAANSDFDLNPGATPGRMPL
                     RPAAVLVPVIAHAAGATVLLTRRADTLTSHSGQIAFPGGRHDPGETAVQTALREANEE
                     VGLDPAVVEVLGIMPAYRTGTGFLITPVVGWIAEAPSVKAQPGEVADMFEVPWDFLMN
                     VANHRRDHLDPPAGPRRWFWAMPFEERYIWGVTAGMIRALHDRLYDAEPNPEAATAED
                     AA"
     gene            complement(2196..2768)
                     /locus_tag="VF633_10835"
     CDS             complement(2196..2768)
                     /locus_tag="VF633_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX8471599.1"
                     /translation="MTDQPTGPHSTGLAAITAAAKQAPGRGLPPVHLWHPEHCGDIDI
                     VVRDDGTWMHEGSPIGRKELVRLFSTVLRLDPDGYHLVTPVEKLKIRVEDLPFRAVVM
                     EQRNDALVFTTDVGDEVVADAEHPIAVDTDPATGEPAPRILVRGGLEARIARTVFYDL
                     VEMAEVVDGRLTVTSAGQRFPLGPVGAGVE"
     gene            complement(2804..3298)
                     /locus_tag="VF633_10840"
     CDS             complement(2804..3298)
                     /locus_tag="VF633_10840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX8471600.1"
                     /translation="MTAAALVPVRLAITPELPSDRGAVEALVLAAFGPGRYAKTAERI
                     REHARMTAGLVARDGDRIVGSVHLWAITVGEVPAVFLGPITVAADHRKGGLGADLVAA
                     CVEVAQQDEVGGVLLVGDAAYFERFGFVAAPDARMPGPVDTRRVLWLPITAPAPSGPV
                     RPVS"
     gene            complement(3332..4108)
                     /locus_tag="VF633_10845"
     CDS             complement(3332..4108)
                     /locus_tag="VF633_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8471601.1"
                     /translation="MSEPIRTEFADGVLTVTLARAEKKNAITQAMYSVLAEATRRAQD
                     DAAVRVLMFKAEGDSFSAGNDIADFIAIGSSGGGQVVDAPVFQFLRSLADLDNPAVAA
                     VRGRAVGIGLTLLLHCDLVVVAEDALLSTPFVNLALAPEAASSLLLPMVIGHQRAFEM
                     FALGEPIDGRTALAWGLANRAVPSAEVDTVAEGLAAKLAVRAPNSIRKTKRLMRDAEQ
                     LWALMQREGEAFGSQLSSPEAMEAFMAFSQKRAPDFSKPG"
     gene            complement(4105..>4388)
                     /locus_tag="VF633_10850"
     CDS             complement(4105..>4388)
                     /locus_tag="VF633_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471602.1"
                     /translation="IEALQRGHETGLARLERTLREPRRAIDVFSSLFARPIGVEVFGM
                     ATGESVAHLNYLERQGRARRERDANGVDWWSATHIKDETGDSAEEETTA"
BASE COUNT          814 a         1504 c         1369 g          701 t
ORIGIN      
        1 gccagcgtgg atggggaagc ctttgcacgg gctgcctgca tcaacagcca accggcggcc
       61 ccgacggtgt aggccagacc cacaccaggg acgaaagcca gcagaccatc cagaccaata
      121 ccgaagggcc cgatcccgac agcccggtca gacagtttct tgacgccctc gacattggac
      181 cagatcttct cgatatctga gatcgtccgc ttggccatct tttcccctcc gcagcgggac
      241 atgcacgttt catcacgccc cgcgttcacc tgaaacgcaa gcgcgcatcc accttgttcg
      301 attgtagctg gacatcaggc cgcctggtct acgcttggag agtctccacc agcgcctcca
      361 ggcgacccgc atggccatga tcgggaaagt cgtcggcgat ccagctgtcc tcgaacgcgg
      421 tcagaacacg acctgtcatc ggacccgccg acaggcccgc cgcagcgacc tgacgtccgc
      481 cgacgggcag gcgtgggatc ggccaggcct cggccagcgc gatcaggttg cttccctgcc
      541 ccgggccacc gctccgggcc tgcgccctta tgacacgatc gatgaaggtt ggacggccaa
      601 ggcggtagat caaggcgcgg gcggcgcgat cgtccatcgt tgtatcgatc tcacctccga
      661 ctgctgcgat cagccggtct cgcgaggcat tggcaagacg caaccggcgc gctgccttct
      721 ccacttgcgc catctctggc gacaagaggg ccgaaagtcg caacacagga tcgggcgaga
      781 gatcgaccat ggtttcgaaa accgcgagcg gccccgcttc cggcaagatc gcatggagaa
      841 ccccggcgct cgccatggcc tggacggttg ggcgaggatc acgcgctgcc agcagcttga
      901 gcagctcttt cgacactcgc tcggcggaga ggcggaccag accctcggac agatcggcac
      961 aagccttcag tcccgccgca tcgggctccc ctcgcccgta ccaggcgaag aaacggaaaa
     1021 agcgcaggat gcgaagatag tcctcacgga tgcgcctctc agggtcgccg acgaagacga
     1081 tctggccctc gcgagcgtca ttcacgccat ggccggtcgg atcgaatacc tgcccttccg
     1141 cgtccgcgta gagcgcgtta aggcgaaagt cgcgacgccc ggcgtcctca gcccagtcgt
     1201 cagtgaaggc gaccacggcc ttgcgcccgt ccgtttcgac atcgcgccgc agagtggtga
     1261 tctcataagg tcgcccgctg gatacggccg taatcgtgcc gtgggccagt cccgtaggca
     1321 cgaccttcag ccccgccttc ttcagcgccc ccatggcgac ctcgggcaac agtcgagtgg
     1381 ccagatctaa atcgtcgacc ttctgcccca tcagggtatt gcgaacacag cccccgacga
     1441 agcgcacgca atccggtccg ccctcagctt gcaaagccgt aagcaccgcg cgtgtcgccg
     1501 gactcttcag ccagggttcg tcagccaccg atgccgtcat gctgcgtcct cggcagtggc
     1561 ggcctcggga ttgggctccg cgtcatagag ccggtcgtgg agcgcgcgga tcatgcccgc
     1621 agtgacgccc cagatgtagc gttcctcgaa cggcatggcc cagaaccagc ggcgcggtcc
     1681 ggcgggcgga tccagatggt cgcgccggtg attggcgaca ttcatcagga agtcccaggg
     1741 aacctcgaac atgtccgcga cctcgcccgg ctgggccttc accgaaggcg cttcagcgat
     1801 ccagccgacc accggcgtga tcagaaaccc ggtgccggtg cgataagcgg gcatgatccc
     1861 cagcacctcg acgacggccg gatccagccc aacttcctcg tttgcctcgc gcaatgctgt
     1921 ctggaccgct gtctcgcccg ggtcatgacg accgccgggg aaggcgatct gaccgctgtg
     1981 gctggtcaag gtgtcggccc ggcgcgtcag caggaccgtg gcgccggcgg catgggcgat
     2041 gaccgggacc aggacagccg caggacgcaa aggcatccgg cccggcgtgg ctcccggatt
     2101 gagatcgaag tcggagttgg ccgcgtcggc gtcagggcgc cagctgtcgg gggagtgcag
     2161 gcgttcgacc agccgcgcct tcagggtcga caggctcatt ccacccccgc cccgacaggt
     2221 cccaacggaa agcgttgtcc cgctgaagtc accgtaaggc gcccgtcaac gacttcagcc
     2281 atctcgacga gatcgtagaa gaccgtgcga gcgatgcggg cttccagacc cccgcgaacc
     2341 agtatgcgag gcgcgggctc gccggtcgct ggatcagtgt cgaccgcgat tggatgttcg
     2401 gcgtcagcaa cgacctcgtc tccgacatcg gttgtgaaga ccagcgcatc attgcgctgt
     2461 tccatgacca ccgcacggaa cggcaggtct tcgacccgga tcttcagctt ttcgacgggt
     2521 gtgaccagat gatagccgtc tggatcgagc cgcaacaccg tggagaacag cctcaccaat
     2581 tccttgcggc cgatgggcga accttcatgc atccaggttc cgtcgtcgcg gaccacgatg
     2641 tcgatgtcgc cgcaatgctc aggatgccag agatgcacgg gaggcaggcc gcgccctggg
     2701 gcctgtttcg cggcggcggt gatcgctgcc aatcctgtgg aatgggggcc tgtcggttga
     2761 tccgtcatgc cccgacttag gcacgccgga gcctcatcgc aaatcaggaa acggggcgca
     2821 ccggccccga cggagccggc gccgtgatcg gcaaccagag gacccggcga gtatctacgg
     2881 ggccgggcat tcgggcgtcc ggggcagcca cgaagccgaa gcgctcgaaa taggcagcat
     2941 cgccgaccag caacacgccg cccacttcgt cctgttgcgc cacctccacg caggccgcga
     3001 ccagatcagc gcccagaccg cccttgcgat gatcggcagc cactgtgatc ggaccgagaa
     3061 agacagccgg gacctcgccg acggtgatgg cccacagatg caccgagcct acgatccgat
     3121 cgccatcgcg tgccaccaga ccagcggtca tgcgggcatg ctcgcggatc cgctcagcgg
     3181 tctttgcata acgaccggga ccaaaggcgg ccagaaccag ggcctcgacc gcaccgcgat
     3241 cagacggaag ttccggcgta atggccagtc ggacaggaac gagggcggcg gcggtcatgg
     3301 cgcgcgcacc taggccctta gtgtgacggg ttcaaccggg tttcgagaaa tccggcgcgc
     3361 gcttctggct gaaagccatg aaggcctcca tcgcctcggg actggacaac tgcgatccga
     3421 acgcctcgcc ctcgcgctgc atcagcgccc aaagctgctc ggcgtcccgc atcagacgct
     3481 tggtcttgcg aatggagttg ggcgcccgca cagccagttt cgcagccaga ccttccgcaa
     3541 ccgtgtctac ctccgcagac ggaacggcgc gattggccaa gccccaggcc agggcagtcc
     3601 ggccatcgat cggctcgccg agagcgaaca tttcaaacgc ccgctgatgg ccgatgacca
     3661 ttggcaacag cagcgacgac gcggcctcgg gcgccagcgc cagattgacg aacggcgtag
     3721 acagcagcgc atcctcggcg accacgacca gatcgcaatg cagcagcagg gtcaggccga
     3781 tgccgaccgc ccggccccgc actgccgcca ctgcaggatt gtccagatcg gccagactac
     3841 ggaggaactg gaacaccggc gcatcgacga cctgtccgcc gccgctggag ccgatggcga
     3901 tgaagtcggc gatgtcgttg ccggcggaga agctgtcgcc ctccgccttg aacatcaaca
     3961 cgcgcactgc ggcgtcgtcc tgagcgcggc gcgtcgcctc ggccagcacc gagtacatcg
     4021 cctgggtgat ggcgttcttc ttctcggcgc gcgcgagggt gacggtcagc acgccatcgg
     4081 cgaattcggt acggatgggt tcgctcatgc ggtcgtttcc tcttcggcgc tgtcgccggt
     4141 ctcgtctttg atatgtgtcg ccgaccacca gtcgacgccg tttgcatccc gctcgcgacg
     4201 cgcccggccc tgtcgttcga gataattcag gtgcgccacg ctctcacctg tggccatgcc
     4261 gaatacctcg accccgattg gccgtgcgaa caggctggaa aacacatcga tggcacggcg
     4321 tggctcacgc aacgtacgtt ccagacgcgc cagtccagtc tcatgtccac gctgcagggc
     4381 ctcgatcc
//
LOCUS       DASVTM010000395         5885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_250645,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000395 DASVTM010000000
VERSION     DASVTM010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250645"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2388
                     /locus_tag="VF633_10855"
     CDS             <1..2388
                     /locus_tag="VF633_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX8471603.1"
                     /translation="ATAMVIVETVALSSMASNSLILPLVARDRWRAREDASDMAGAIL
                     QVRRVVICGILFLAWLYFQVIDRSVGLASIGLTSFAALAQLAPALFGAVLWRGGHARG
                     AIAGILVGSGLWLVLLALPQIGTTLGWPRLQLFALDDPLPLGVFLSLSANTLCYVLIS
                     KSVRPRLIDRVQARAFVDRLSPDWREQSTAPSGASVGDLKTLVSRFVGDAGAERAFAA
                     FGREANQSLKDNDPADAGLARAAERMLAGAVGASSARRVISAALAGGGRAPEDVVRML
                     DEASQAVQFNRELLQTTLDNIDQGVSVVDEDLRLIAWNARYIELFDLPSGFVHVGQPV
                     AAVYRLNAERGEVEPEDMEAWIERRLDALRRRSPHDHERAQPGGRILKSTGAPMSGGG
                     YVTSYTDITELRRAAQALEEANEQLEARVADRTERLVEAKQVAEDATASKTRFLAAAS
                     HDLLQPLHAARLFIGALKEGPGSQDPETRKLVENADRSIESAHGLLKALLNLSRLEAG
                     GVRPAVTALSVETLFGDLRREFTLIAREKGLRLTIVPCGQWVLSDRDLLRSLLQNLVG
                     NAIRYTDSGRVLVGARRVEGVIRIEVFDTGRGIAPDQHEAIFTEFTRLPNQGGDEPGV
                     GLGLAIVRKVASLLDHPLTLRSSPGRGSVFSVTAPRTGASLAQMPSSGASRSALAGLR
                     VLCVDNEPIILEALGALLTRWGMTVVTAPDAASALAVTGPFDAALVDLHLGAGPDGCF
                     VIEALRARGVGHLALISADTDETLPARAAAVGAVLLPKPVKPATLKAFLSSPGRT"
     gene            complement(2410..3027)
                     /locus_tag="VF633_10860"
     CDS             complement(2410..3027)
                     /locus_tag="VF633_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8471604.1"
                     /translation="MDRIVIADDHPMFRAALATAVARAAPGAVIEETASLAGARQALE
                     AGAADLVLLDLKLSDSEGFAGLAGIRADYPAVAVAVVSATEDDATIRRALAFGAAGFI
                     PKSAALAQMVEALEAILAGDVWAPAIPQTAPADSFEARIVSLTPSQLKILIGLQQGRL
                     NKQIAFDLGVTEATIKAHLTVVFRKLGVQNRTQAVIAAQALGPEA"
     gene            3187..4539
                     /locus_tag="VF633_10865"
     CDS             3187..4539
                     /locus_tag="VF633_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HEX8471605.1"
                     /translation="MIRRTLMAGAAAVALFTAPGLAVAQAQDTTALEARIALLEAQLN
                     DLKSEIVASRTQQTAQAQDIIRIDQRVAAPPAPIPVSAPADGFRIGNNTLKIGGFVKA
                     DFIASNYDGGDPANGDLLRDFYLPGSVPVGAGDESTATDFNARQTRFWLTTEGLLGGH
                     KVGTRVEMDFQVLPGTGDQRTTSPSNLALRRAFVTVDNWLFGQEWTNFQNIAVLPDTA
                     DYIGASEGTVFARQVQVRYTRGPLSISVENPETTVTPFLGATRIIADDNSLPDVTARY
                     VLARPWGEASLSGIVRQLAYETTGVGAIDSSTMGWGLSAAAKIKVGAKDDLRVMLSGG
                     EGIGRYVGLNFANDAVLDASGELEAIGLIAGFASYRHVWSPQWRSNLTYSAQNVDNDL
                     SLIGVATNKSAQSIRGNLIWTPLPALDLGAELMFGERELESGVSGELTRLQVFAKYGF
                     "
     gene            4613..>5885
                     /locus_tag="VF633_10870"
     CDS             4613..>5885
                     /locus_tag="VF633_10870"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX8471606.1"
                     /translation="MDAPAYEAACIAARETPDAFWAEAAKRLDWITPPTIIKDVSFHK
                     DDFRIRWFADGVLNVAYNCVDRHLAERGDQTAILFEGDDPSVSGAITYAELHLRVCRM
                     ANVLKDLGVRKGDRVTIYLPMCPAAAVAMLACARIGAVHSVVFGGFSPDSIAGRIEDC
                     GSNIVITADEGLRAGKRIPLKANVDEALTKVSVAKVLVIAHTGADVPLVEGRDVRFED
                     AVKTVADDCPCEPMNAEDPLFILYTSGSTGKPKGVMHTTGGYLFWASWTHELVFDYRP
                     GEVFWCTADVGWVTGHSYVVYGALANAATTLMFEGVPNYPDNGRFWQVVDKHKVEIFY
                     TAPTALRALMRDGDAPVKASSRKSLRLLGSVGEPINPEAWRWYHEVVGDSRLPIVDTW
                     WQTETGGIMVSPLPGATDLKPGSATKPLPGVA"
BASE COUNT          896 a         2135 c         1885 g          969 t
ORIGIN      
        1 gccacggcca tggtcatcgt cgagacggtc gccctgtcct ccatggcctc caacagcctg
       61 atcctgccgc tggtggctcg ggatcgctgg cgggctcgcg aggatgcgtc ggacatggcc
      121 ggcgcgatcc tgcaggtccg ccgggtcgtg atttgcggca tcctgttcct ggcgtggctt
      181 tattttcagg tcatcgaccg ttccgtcggc ctcgcctcga tcggcctgac ctccttcgcc
      241 gccctcgccc agctggcccc ggccctgttc ggcgctgtgc tgtggcgcgg aggccatgca
      301 cggggcgcca tcgccgggat tctggtcggc tcgggcctgt ggctggtgct gcttgccttg
      361 ccccagatcg gcacgacgct gggctggccg aggttgcagc ttttcgctct cgacgacccg
      421 ttgccgctcg gcgtcttcct cagccttagc gcgaatacgc tctgttacgt tctcatttcg
      481 aagtcggttc gcccccgttt gatcgaccgc gtccaggcgc gcgccttcgt cgatcgcctg
      541 agcccggact ggcgcgagca aagcacggct ccctcgggcg cgtcggtcgg cgatctgaag
      601 accctcgtct cccgcttcgt cggtgatgcc ggggccgagc gcgccttcgc ggccttcggg
      661 cgagaggcga accagtcctt gaaagacaac gatcccgccg atgccgggct ggcccgcgcc
      721 gccgaacgga tgctggccgg ggccgtcggc gcctcgtctg cccgacgggt catctcggcg
      781 gccctcgccg gtggtggccg cgcgcctgag gacgtggtgc gcatgctcga cgaggcctca
      841 caggcagtcc agttcaatcg cgaactgctt caaaccacgc tcgacaacat cgaccaaggc
      901 gtcagcgtgg tcgacgagga cctgcgcctg atcgcctgga atgcgcgata tatcgagctg
      961 ttcgatctgc cgtccggctt cgtccatgtg ggtcagccgg tcgccgccgt ctatcgcctg
     1021 aacgccgaga ggggggaggt cgagcctgag gatatggagg cgtggatcga acgccgtctg
     1081 gacgcccttc gccgtcgcag cccccacgat cacgaacgcg ctcagcccgg cggccggatc
     1141 ctgaagtcca ccggcgcccc gatgtccggc ggcggctatg tcacctctta taccgacatc
     1201 accgaactgc gccgcgccgc gcaggcgctc gaagaggcca acgaacagct cgaggcgcgc
     1261 gtggccgacc gcaccgagcg gctggtcgag gccaaacagg tggccgaaga cgccacagcc
     1321 tccaagaccc gcttcctcgc cgccgccagc cacgacctgc tgcaacccct gcacgccgcc
     1381 cgcctgttca tcggcgcgct caaggaaggc cccggctcgc aagaccccga aacgcgcaag
     1441 ctggtggaga acgccgaccg ttcgatcgag tcggcgcacg gcctgctcaa ggcgctgctg
     1501 aacctctcgc ggctcgaggc cgggggcgtg cgccctgcgg tgacggccct ctcggtggag
     1561 acactattcg gcgatctccg tcgcgagttc accctcattg cgcgcgagaa aggcctgcgc
     1621 ctgaccatcg ttccctgcgg tcaatgggtc ctgtctgacc gcgacctgct gcgctctctg
     1681 ctgcaaaatc tggtcggcaa cgccatccgg tacacagaca gcggccgtgt cctcgtcggg
     1741 gcgcgaaggg tcgaaggcgt gatccgcatc gaggtgttcg acaccggccg aggcatcgcc
     1801 cccgaccagc acgaggcgat cttcaccgag ttcacccgcc tgcccaacca gggcggcgac
     1861 gagcccggtg tcggtctggg cctcgccatc gttcgcaagg tcgccagtct gctggatcat
     1921 cccctgaccc tccgctcctc gcccggccgg ggttcggtgt tttcggtgac ggcgccgcgc
     1981 accggggcgt ccctcgcgca aatgccctca tcaggggcca gccggagcgc cctcgccggc
     2041 ttgcgggttc tgtgcgtcga caatgagccg atcattctgg aggcgttagg cgccctcctc
     2101 acccgctggg gcatgacggt ggtcaccgcc cccgacgcag cctccgccct tgcggtgacc
     2161 ggcccgttcg acgccgccct ggtcgatctg catctgggag cgggtcctga cggttgtttc
     2221 gtcatcgagg ccctcagggc gcgcggcgtg ggccatctgg ccctgatcag cgccgacacc
     2281 gacgaaacct tgcccgcccg cgccgccgcc gtgggcgccg tgcttttgcc gaagccggtc
     2341 aaacctgcga ccttgaaggc tttcctctcc agtcccggcc gcacctgacg ccgggatcag
     2401 gtctcgggat caggcctcgg gacccagcgc ctgggcggcg atcaccgcct gggtccggtt
     2461 ctggaccccg agctttctga acaccaccgt caggtgcgcc ttgatcgtcg cctcggtcac
     2521 cccgaggtcg aaggcgatct gcttgttcag ccgcccctgc tgcaggccga tcaggatctt
     2581 caactgagac ggcgtcaggc ttacgatacg cgcctcgaac gagtccgcag gggcggtctg
     2641 cgggatcgcc ggagcccaga cgtcgccggc cagaatggcc tcgagcgctt cgaccatctg
     2701 ggccagagcc gccgacttgg gaatgaagcc cgccgcgccg aacgccagcg cgcgccggat
     2761 ggtcgcgtcg tcctcggtgg ccgagaccac cgccaccgcc accgccggat agtcggcccg
     2821 gatgccggcc agtcccgcga aaccttcgct gtccgacagt ttgagatcga gcaggaccag
     2881 atccgccgct cccgcctcca gagcctggcg agcgccggcc agactggcgg tctcctcgat
     2941 gaccgcgccg ggcgccgccc gcgccacggc ggtcgccagt gcggcgcgga acatcggatg
     3001 gtcgtcggcg atgacgatgc ggtccatggc ggtcctcccc gggccgagcc ctgacggcgc
     3061 gcgcgatgcg ccagtctgac accccccgac tttagtcgaa actagaccaa cggctaatgg
     3121 tcccgcatgc ggtccgtgtc gaaaggtaag cgcaggtctg caccagatgg accccgggag
     3181 gacaccatga tcagacgaac cctgatggcc ggcgccgccg ccgtcgccct gttcaccgcg
     3241 cccggcctgg ccgtcgcaca ggcccaggac accaccgcgc tggaggcccg catcgccctg
     3301 ctggaggccc agctgaacga cctcaagtcc gagatcgtgg cctcgcgcac ccagcagacg
     3361 gcgcaggctc aggacatcat ccgcatcgac cagcgcgtcg cggccccgcc cgcccccatc
     3421 cccgtgtccg ccccggccga cggcttccgc atcggcaaca acaccctcaa gatcggcggc
     3481 tttgtgaagg ccgacttcat cgcctccaat tatgacggcg gcgatccggc caacggcgac
     3541 ctgctgcgcg acttctacct tccgggatcg gtccccgtcg gggccggcga cgagagcacg
     3601 gcgaccgact tcaacgcccg tcagacccgg ttctggctga ccaccgaagg tcttctggga
     3661 ggccacaagg tcggcacccg tgtggagatg gacttccagg tcttgcccgg cacgggcgac
     3721 cagcgcacca ccagcccgtc caacctcgcc ctgcgccgcg ccttcgtcac cgtcgacaac
     3781 tggctgttcg gtcaggaatg gaccaatttc cagaacatcg ccgtcctgcc agacaccgcc
     3841 gactatatcg gggcctcgga gggcaccgtg ttcgcgcgtc aggtccaggt ccgctatacg
     3901 cgcggcccgc tctcgatctc ggtcgagaac ccggagacga ccgtcacccc cttcctcggc
     3961 gcgacccgca tcatcgccga cgacaacagc ctgcccgacg tcaccgcccg ctatgtgctc
     4021 gcccggccgt ggggcgaggc ctcgctgtcc ggcatcgtgc gccagctggc ctatgaaacc
     4081 accggcgtgg gcgcgatcga ctcctcgacg atgggctggg gtctgtccgc cgccgccaag
     4141 atcaaggtcg gggccaagga cgacctgcgc gtcatgctgt ccggcggcga gggcatcggc
     4201 cgctatgtcg gtctgaactt cgccaacgac gccgtgctgg acgcctcggg cgagctggag
     4261 gccatcggcc tgatcgccgg cttcgccagc tatcgccacg tctggtcgcc ccaatggcgc
     4321 tcgaacctga cctattcggc ccagaatgtg gacaacgacc tgtcgctgat cggcgtcgcc
     4381 accaacaaat cggcccagag tattcggggc aacctgatct ggacgcccct gcccgcgctg
     4441 gacctgggcg ccgagttgat gttcggtgag cgtgagctgg aatcgggcgt ctcgggcgag
     4501 ctgacccgtc tgcaggtctt cgccaaatac ggtttctgag cctgaagggt aaaagcgttt
     4561 gagcgatccg gaactctatc ccgtccccgc cgaccgggcg gcgcgcgccc acatggacgc
     4621 gcccgcctat gaggctgcct gcatcgctgc gcgcgagacg ccggacgcct tctgggccga
     4681 ggcggcgaaa cgtctggact ggatcacgcc gccgacgatc atcaaggacg tcagcttcca
     4741 caaggacgac ttccgcatcc gctggttcgc cgacggggtg ctgaacgtcg cgtacaactg
     4801 cgtcgaccgg catctggccg agcgcggcga ccagacggcg atcctgttcg agggcgacga
     4861 tccctcggtg tcgggcgcca tcacctatgc cgagctgcac cttcgggtct gccgcatggc
     4921 caatgtgctg aaagatctcg gtgtccggaa gggcgaccgc gtcaccatct atctgccgat
     4981 gtgccccgcc gccgccgtcg ccatgctggc ctgcgcccgc atcggcgcgg tgcattcggt
     5041 ggtcttcggc ggcttctcgc ccgacagcat cgccgggcgg atcgaggact gcggctccaa
     5101 catcgtcatc accgccgacg agggcctgcg cgccggcaaa cgcatcccgc tcaaggccaa
     5161 tgtcgacgag gctctcacca aggtttcggt cgccaaggtg ctggtcatcg cccacaccgg
     5221 cgccgacgtg ccgctggtcg agggccgcga cgtccgcttc gaggatgcgg taaagaccgt
     5281 cgccgacgac tgcccgtgcg agccaatgaa cgccgaggat ccgctgttca tcctctacac
     5341 ctcgggctcg accggtaagc ccaagggggt gatgcacacc acgggcggct atctgttctg
     5401 ggcgtcatgg acgcacgaac tggtgttcga ctaccgcccc ggcgaggtgt tctggtgcac
     5461 cgccgacgtc ggctgggtca cgggccacag ctacgtcgtc tatggcgcgc tcgccaatgc
     5521 cgcgacgacc ctgatgttcg agggcgtgcc caactatccc gacaacggcc gcttctggca
     5581 ggtggtcgac aagcacaagg tcgaaatctt ctacaccgcg cctactgcac taagagcgtt
     5641 gatgcgcgac ggcgatgcgc cggtgaaggc ctcgagccgc aagtccttgc gcctgctcgg
     5701 cagcgtcggt gagccgatca accccgaggc ctggcgctgg tatcatgagg tcgtcggcga
     5761 cagtcgcctg ccgatcgtgg acacctggtg gcagaccgag accggcggca tcatggtctc
     5821 ccccctgccc ggcgccaccg acctcaagcc cggctcggcg accaaacccc tgcccggcgt
     5881 cgcgc
//
LOCUS       DASVTM010000396         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_462609,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000396 DASVTM010000000
VERSION     DASVTM010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462609"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1721
                     /locus_tag="VF633_10875"
     CDS             <1..>1721
                     /locus_tag="VF633_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471607.1"
                     /translation="LAQQADQHEEEAAEVDEVVVQATRSGRRIQNEPIRVEVINREEI
                     EEKALMSPGNIAMLLNETGGVRVQITSPALGAANIRLQGMRGRYTQLLADGLPLYGGQ
                     ASSLGVLQIPPMDLGRVEVIKGAASALYGASALGGVINLVSRQPGDEAEGDLLINLTS
                     RDGQDLTAYAATPFNDAWSGSIIGGYHRQSRQDLDSDGWSDIAAYERWTVRPRLFWDG
                     ENGATVFATVGAMIEDRVGGTEPGRTVPDGRPFVQAQDTNRLDAGLVASTPVGGGLTA
                     HLRASVMTQDHDHQFGDVFETDRHDTSFIEAALGSGNGPTTWLAGVAVQRDGYRSEAF
                     PGFDYAFTVPGVFAQVEHDLTDDLTLAGSARLDDHSQYGTKFSPRLSVLYRPGRWTIR
                     ASLGQGFYGPTPFVEEIEANGLSRLLPLGDLKAETARTASLDLGWKQGPIEANLTVFG
                     SDLDDAIRLADDGPARVRLVNVDGVTRTRGAEALLRYRVAPFTVTGSYVFVDAMEPLD
                     SGTGRQAVPLTPRHTAGLVAMWEEHDRGRLGLEIYYTGPQTLDDNPYRSKSRSYVEVG
                     LMGEVVLG"
BASE COUNT          311 a          548 c          574 g          288 t
ORIGIN      
        1 ctcgcgcagc aagccgatca gcacgaggaa gaggcggcgg aagtcgatga ggtagtggtt
       61 caggccaccc gatcaggccg ccgcatccag aacgaaccca ttcgcgtcga ggtcatcaat
      121 cgcgaggaaa tcgaggagaa ggcgctgatg tcgcccggca acatcgccat gttgctgaac
      181 gagacgggcg gtgtgcgcgt gcagatcacc tcacccgcgc ttggcgcagc gaacatccgc
      241 cttcagggga tgagggggcg ctacacccaa ctgctcgcgg acggcttgcc gctctacgga
      301 ggacaggcgt cttcattggg ggtgctgcaa atccctccga tggaccttgg gcgggtcgag
      361 gttatcaagg gggcagcttc ggccctgtat ggcgcgtcgg cattgggcgg tgtcatcaat
      421 ctggtttcgc gccaaccggg cgacgaagca gagggcgacc tcctgatcaa cctgaccagc
      481 cgtgacggtc aggacctgac cgcctacgca gccacaccgt tcaacgatgc ctggagcggt
      541 tcgatcatcg gcggctatca tcgccagtcg cgacaagacc tggacagcga tggctggagt
      601 gacatcgccg cctatgagcg gtggacggtc aggccgcgtc tgttctggga cggagagaac
      661 ggagcgactg tcttcgcaac ggtcggagcc atgatcgaag atcgcgtcgg aggcactgag
      721 ccggggcgca ccgtccccga cggccgcccg tttgttcagg cgcaggacac caaccgcctc
      781 gacgccggcc tggtcgcctc cacccctgtc gggggcgggc tcacggcgca cctgcgagcc
      841 tcggtcatga cacaggatca cgaccaccag ttcggagacg tgttcgagac cgaccgccac
      901 gacaccagtt tcatcgaggc cgcgctgggg tcaggaaacg gtccgacgac atggctggct
      961 ggcgtcgcgg tgcaacggga cggctaccgg tccgaggcct tcccgggctt cgactacgcc
     1021 ttcaccgtcc ccggcgtctt cgcccaggtt gagcatgacc tgaccgacga tctgaccttg
     1081 gccggaagcg caaggcttga cgaccacagc caatacggga cgaagttcag cccgcgtctg
     1141 tcggttctct atcgaccggg ccggtggacg attcgcgctt cgctggggca aggcttttac
     1201 ggcccgacgc ctttcgtcga ggagatcgag gcgaacggct tgtctcgtct cttgcccttg
     1261 ggcgatctga aagccgagac ggctcgcact gcctcgctcg atctcggatg gaagcaaggg
     1321 ccgatcgagg ccaacctgac ggtcttcggc tccgatctgg acgacgccat ccggctggcg
     1381 gacgacggtc cggcgcgcgt tcggttggtc aacgtcgacg gcgtaacgcg cacgcggggc
     1441 gccgaagccc tgctgcgcta tcgcgtcgcg ccattcaccg tcaccggaag ctatgtcttc
     1501 gtggacgcga tggaaccgct cgacagcggg acaggccgcc aggccgtgcc attgaccccg
     1561 cgccacacgg ctggcctggt cgccatgtgg gaggaacacg accgtggcag gctggggctc
     1621 gaaatctact acaccggacc acagacgctg gatgacaatc cctaccgttc gaagagccgg
     1681 tcctatgtcg aggtcgggct gatgggcgag gtcgttcttg g
//
LOCUS       DASVTM010000397         6396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_251002,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000397 DASVTM010000000
VERSION     DASVTM010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251002"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VF633_10880"
     CDS             complement(<1..349)
                     /locus_tag="VF633_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8471608.1"
                     /translation="MNSRLIRPVSLVRHLGAWRAPGAGAAWRQLAGALRLLILDGRLP
                     LEARLPGERELAQALDLSRTTVSSAYGMLRDEGFLTGGRGEAVRTTLPSGPRPRAPGE
                     ARPLPAADVIEMTS"
     gene            410..1036
                     /locus_tag="VF633_10885"
     CDS             410..1036
                     /locus_tag="VF633_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471609.1"
                     /translation="MIRRLIQLFVGLGLYGFSIALIVRADLGLDPWDVLNQGVFERFA
                     GPFGVSFGLVVNLIGLAVLLLWIPLRQMPGIGTVANVLVIGTIANVGLQWIPADLPLI
                     LRAGLLLAGIVLTGVASGAYIGAGLGPGPRDGLMTGIVRLTGWPVKWVRTAIEIAVVV
                     VGWTLGGSIGVGTILYAVSIGPLVHVFLPMFTIRPPEPPVLLESAPHS"
     gene            complement(1148..1834)
                     /locus_tag="VF633_10890"
     CDS             complement(1148..1834)
                     /locus_tag="VF633_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519232.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8471610.1"
                     /translation="MPTPKTLLIIDDDDDLREALAEQLALHEEFRTVQAATATEGIRL
                     GRELRADLILLDVDLPDMDGREACRLLRKDGVATPVIMLTAQDSDADTVLGLDSGAND
                     YVTKPFRFAVLLARIRAHLRSHEQSEDAVFAIGPYSFRPAGKMLVDDKGKKIRLTEKE
                     TNILKYLYRAGAKPVSREELLTEVWGYNAGVTTHTLETHIYRLRQKIEPEPGAARLLL
                     TDAGGYRLQP"
     gene            complement(1914..2627)
                     /locus_tag="VF633_10895"
     CDS             complement(1914..2627)
                     /locus_tag="VF633_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX8471611.1"
                     /translation="MSPSSPGFDAPSMAPASIADRVTAVRARIAETARSVGRDPASVT
                     LTAVSKTQQPEAIDAILTTGQRIFGENRVQEAQGRWTERRLDVPNLQLRLIGPLQSNK
                     AEDAVALFDVIETLDREKLARTLADAIQSAGRAPKMLVQVNTGAEPQKAGILPKEADA
                     FIALAGETYGLQIDGLMAIPPADEAPGLHFALLAKIAERNGLGVLSMGMSGDYETAIR
                     FGATHVRVGTALFGERTPA"
     gene            2626..3267
                     /locus_tag="VF633_10900"
     CDS             2626..3267
                     /locus_tag="VF633_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX8471612.1"
                     /translation="MDGSGTLSHDARLLWDTAEGLARAAHRVSARALPPLLFFTDPER
                     TPEPWRTAERLPEGAGVVYRHFGATDAGATARRLKDITVERGLILLIGLDADLAEAVQ
                     ADGVHLPERALDQATSLRARRPDWILTGAAHSIDTTLASKDLDAVILSPIFPAGGASA
                     VKPSLGIEALIAVSARRPVYALGGVGPKTVGALASTGACGIAGVDAIQDAFTA"
     gene            complement(3286..3696)
                     /locus_tag="VF633_10905"
     CDS             complement(3286..3696)
                     /locus_tag="VF633_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471613.1"
                     /translation="MRFGAIFAGLLSAALLMGAAQTASAQSVGARSRTSAVSLAEAQA
                     AQASQRGNPPPARRGLRWYDTGRWGLNFNLNQPVGRETQWGDVEAGAYYSVTPRLSVG
                     AAAGLARPEQDPARAPADDNRTQPRIRLESIFKF"
     gene            4112..4570
                     /locus_tag="VF633_10910"
     CDS             4112..4570
                     /locus_tag="VF633_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEX8471614.1"
                     /translation="MALNKAMVRGATIAAIASGLMLSACGGVKVPFVGGPSKPKANAQ
                     AGIGVNAYLWRATLDTLSFMPLLTADPWGGVVNYDWYINPQTPNERFKATVFILDTRL
                     RADALNVTVTKEVKDASGGWTAAPVAAQTETDLENAILTKARQLNLANAN"
     gene            4752..>6396
                     /gene="leuS"
                     /locus_tag="VF633_10915"
     CDS             4752..>6396
                     /gene="leuS"
                     /locus_tag="VF633_10915"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX8471615.1"
                     /translation="MARYEPKTAEPRQQQRWSDANAFVTKDTGRPKYYVLEMFPYPSG
                     AIHMGHARNYVMGDVVARHKRAQGFDVLHPMGWDAFGMPAENAAMERGIHPKGWTYSN
                     IATMREQLKLLGLSLDWSREFATCDPEYYGKQQAWFLELFKRGLVYRKDGVVNWDPVD
                     NTVLANEQVIDGKGWRSGAVVEKRKLNQWFLRITDYADDLIEGLKDLDRWPDKVRLMQ
                     ENWIGRSKGLQMTWKWAGDAPPSLSGAASPDGLEIYTTRPDTLFGASFMGIAPDHPIA
                     RAMAEADPAVAEFTAKCRAGGTSQADIEQAEKIGWDTGLKVTHPFDGREVSVWIANFI
                     LSEYGTGAIFGCPAHDQRDLDFARKYDLPVIPVVKPADAADDFAVENEAYVGPGALFH
                     SDFLDGMSIEDGKAAAIQRIEDAGMGKGATIYRLRDWGVSRQRYWGCPIPIIHCPSCG
                     VQPVPADQLPVILPDDVTFDVPGNPLDRHPTWKHVKCPACNEDATRETDTLDTFVDSS
                     WYFARFTDPTADAPIDKAAADHWLAVDQYIGGVEHAVLHL"
BASE COUNT         1061 a         2084 c         2095 g         1156 t
ORIGIN      
        1 cggaggtcat ctcgatgaca tccgcagccg gcaaggggcg ggcctcgccc ggggctcgcg
       61 gtcgcggacc tgaaggcaag gtggtgcgga ccgcctcgcc ccggccaccg gtcaggaagc
      121 cttcgtcgcg caacatgcca taggcggacg agaccgtggt ccggctgagg tccaacgcct
      181 gcgccagttc gcgctctccc ggcagtcgcg cttccagcgg cagccgcccg tcgaggatca
      241 gcaaacgcag cgctccggcc agttgccgcc acgccgctcc ggcgccgggc gcgcgccagg
      301 cacccagatg gcgaacgagt gaaacgggtc ggatcaggcg ggagttcatc agtccagact
      361 accgcaactg gctctttctt tcatagccag tacagcgcaa atcagcatca tgatccgtcg
      421 tctcatccag ctctttgtcg gccttggcct ctacggcttc tcgatcgcgc tgatcgtccg
      481 cgcggacctc ggcctcgacc cctgggacgt gctcaatcag ggtgtgttcg agcgtttcgc
      541 ggggccgttc ggcgtcagct tcgggctggt ggtcaatctg atcgggctgg cggtcttgct
      601 gctctggata ccgcttcgcc agatgcccgg catcggaacc gtggccaatg tgctggtcat
      661 cggcacgatc gccaatgtcg gcctgcaatg gataccggcc gatctgccct tgatcctgcg
      721 cgctggtctt ctgctcgctg gtatcgtgct gactggtgtg gcgagcggcg cctacatcgg
      781 tgcaggcttg ggccccggcc cgcgcgacgg cctgatgacg ggcatcgtgc gtctcaccgg
      841 ctggccggtg aaatgggtgc gcacggcgat cgagatcgcg gttgtcgtgg tcggctggac
      901 gctggggggc tcgatcggcg tggggaccat cctctacgcg gtaagcatcg gaccactggt
      961 ccacgtcttc ctgccgatgt tcacgatccg cccgcccgaa ccgccagtcc tgctggagag
     1021 cgcgcctcat tcgtgacctg agatcacagg ttctggtgct cgacgcggct agcaccgtga
     1081 cgtcagccta tctcaactac ccatactggg gcgaaggcgg cgtccgttag cttaacccgc
     1141 cacccctcta gggctgcagg cgatacccgc cagcgtcggt gagcagcagt ctcgccgcgc
     1201 cgggctcggg ctcgatcttc tggcgcaggc gatagatgtg ggtttccagc gtgtgggtcg
     1261 tgaccccggc gttatagccc cagacctcgg tcagcagttc ctcgcgcgac acgggcttgg
     1321 ccccggcgcg atagaggtat ttgaggatat tggtctcttt ttccgtcagc cggattttct
     1381 tgcccttgtc gtcgaccagc atcttgccag cgggacggaa ggaatagggg ccgatcgcga
     1441 agaccgcgtc ctcggactgc tcgtgactgc gcaggtgggc gcggatgcgg gccagcagga
     1501 cagcaaaccg gaagggcttg gtcacatagt cgttggcgcc cgagtcgaga cccagaacgg
     1561 tgtcggcgtc cgagtcctga gccgtcagca tgatcactgg ggtagcgacc ccgtccttgc
     1621 gcaacaggcg gcaggcctcg cggccgtcca tgtcgggcag gtcgacgtcc agcaggatca
     1681 ggtcagcgcg cagctcacga ccgaggcgga tgccctcggt ggcggtcgcg gcctggaccg
     1741 tgcggaactc ttcatgaagc gccagctgct cggccaaggc ctcgcgcagg tcgtcgtcgt
     1801 cgtcgatgat cagcagcgtc ttgggagtgg gcatggttgt tacaatggcg aggcttgggt
     1861 gaatcgccaa gggcgtgccg gaaaatcaag acgattcagg caatagagcg ggcttaagcg
     1921 ggggttcgtt ccccgaaaag cgccgttccg acccgaacat gggtcgcgcc gaaccgaatc
     1981 gccgtctcgt aatcgccgct cattcccatc gacaggacgc ccagaccgtt gcgttcggcg
     2041 atcttcgcca gcagcgcaaa atgcagcccg ggcgcctcgt cagcgggcgg aatggccatc
     2101 agtccatcga tttgcaagcc ataggtttcg cccgccaggg cgatgaatgc gtcggcctct
     2161 ttgggcagga taccggcctt ctgcggctcg gcgccggtgt tgacctgaac cagcatcttc
     2221 ggcgcacgtc cggcggactg gatggcatct gcgagcgtcc gcgccagctt ttcacggtcg
     2281 agcgtctcga tgacgtcgaa caaggcgacc gcgtcctcgg ccttgttcga ttgcagcggc
     2341 ccgatcagac gcagctgcag attggggacg tcgaggcgtc gctcggtcca gcgaccttgc
     2401 gcctcctgca cccggttctc gccgaagatg cgctggccgg tggtgaggat ggcgtcgatg
     2461 gcttccggct gctgggtctt ggatacggct gtcaaggtga cggaggcagg gtcgcgcccg
     2521 accgagcggg cggtctccgc aatgcgggcc ctgaccgcag tgacgcggtc agcgatgctc
     2581 gccggggcca tggacggggc gtcgaaaccg ggggatgacg gggacatgga tggctccgga
     2641 actctgtcgc acgatgcgcg cctgctgtgg gacacggccg agggtctagc ccgggcggcc
     2701 catcgtgtca gcgcccgcgc gctgccgccg ctgctgttct tcaccgatcc cgagcgaacc
     2761 cccgagccgt ggcgcactgc agaacgcctg ccggaaggcg ctggcgtcgt ttatcggcat
     2821 ttcggcgcca cagacgccgg agccacggcg cgacggctca aggacatcac cgtcgagcga
     2881 ggactgatcc tgctgatagg gctggatgct gatctggccg aggccgtcca ggccgatggc
     2941 gttcatttgc ccgaacgagc gctggatcag gcgacatcgc tgcgagcgcg gcggccagac
     3001 tggatcctga ctggagcggc gcattccata gatacgacac tcgcatccaa ggatctggac
     3061 gccgtgatcc tgtcgccgat tttcccggcg ggcggcgcct cggcggtcaa gccgtcgttg
     3121 ggaatcgaag ccttgatcgc ggtgtcagcg cgcaggcctg tctatgctct gggcggggta
     3181 gggccgaaga ccgtcggcgc gctggcttca accggcgcat gcggaatcgc cggagtcgat
     3241 gcgatacagg acgctttcac cgcctgaagc ggctgcagtc agcctttaga acttgaagat
     3301 ggattccagc cggatgcgcg gttgggtgcg gttatcatcg gccggggcgc gggccgggtc
     3361 ctgttccgga cgggccagac cggcggcggc cccgacgctc aggcgcggcg tgacgctgta
     3421 ataggcgccc gcctcgacat cgccccactg ggtctcgcga ccgacgggct gattgaggtt
     3481 gaagttcaga ccccagcgac cggtgtcata ccagcgcagg ccgcgacgtg cagggggcgg
     3541 atttccacgt tgggacgctt gagcagcctg agcctcggcg agcgaaacgg cagacgtgcg
     3601 cgatcgcgca ccgacggatt gcgccgatgc agtctgggcc gctcccatca ggagcgccgc
     3661 gctcaatagt cctgcgaaga tcgcgccgaa ccgcatttcc caacccttca gacgcgacca
     3721 ggcgcgtcat caaatcctga accggcgttt tcgccgtgaa cccaaacgat tagacagcct
     3781 gggttcgccc ggtcaacgca acgtggtcgc ttctcccgac ttcttccgac tctaggaggc
     3841 ccctgtggcg caagcgtcac gggattgcgg cggaaactcg tacatcgctt tcggcaccaa
     3901 cgcgggtctg catccggagg cttcctaaac tcatgttgca caactgcaaa gatcgcggcg
     3961 ttcttcgcct tgtcattgcc ccttttcgac gtgttataga gcctcgttgc ggccttcgtc
     4021 ctgaccctgg aatggggggc ggagctgttg cgcccgggcg acgtgtgctc acccgcgggt
     4081 ccggcgtcca atctgaacgg agaattcctt gatggccctc aataaagcga tggttcgcgg
     4141 tgctacgatc gccgccattg cgtccggcct gatgctgtcc gcctgcggag gcgtgaaagt
     4201 gcctttcgtc ggcggtccct ccaagcccaa ggccaatgcc caggcgggta tcggcgtgaa
     4261 cgcctatctg tggcgtgcga cgctcgacac cctcagcttc atgccgctgc tgactgctga
     4321 tccgtggggc ggcgtcgtga actacgactg gtacatcaat ccccagacgc cgaacgagcg
     4381 tttcaaggcg acggtcttca tcctcgacac gcgcctgcgc gccgatgcgt tgaacgtgac
     4441 cgtgaccaag gaagtcaagg acgcctcggg cggctggact gctgcgcccg tcgccgccca
     4501 gaccgagacc gatctcgaga acgcgatcct gaccaaggcc cgtcagctca atctggccaa
     4561 cgccaactag gcatcgttct ttagggcgct atcgctcgtg acgcggtcgc ttgctgcttg
     4621 agcgccttct gtgtgtttcg cccttcgctg cttcggcacg aagggcgtct ctaatttcct
     4681 tgggcgtcgc cgcccttcgg gctaactgag cgcgaggggc gtggccctat cgccgtctct
     4741 tcaggatgcc cgtggcccgt tacgaaccca aaaccgccga accccgtcag cagcagcgct
     4801 ggtcggatgc gaacgccttc gtcacgaagg ataccgggcg gccgaaatac tatgtgctcg
     4861 agatgttccc gtatccgtcg ggcgccatcc acatgggtca tgcccgcaac tatgtgatgg
     4921 gcgacgtggt cgcgcgtcac aaacgggcgc agggcttcga tgtgctgcat ccgatgggct
     4981 gggacgcctt cggcatgccg gccgagaacg cggccatgga acgcggcatc caccccaagg
     5041 gctggaccta ttccaacatc gccacgatgc gcgagcagtt gaagctgttg ggcctgtcgc
     5101 tggactggtc gcgcgagttc gcgacctgcg atcccgaata ctacggcaag caacaggcct
     5161 ggttcctcga gctgttcaag cgtgggctcg tctatcgcaa ggacggcgtg gtcaactggg
     5221 atccggtcga caacaccgtc ctggccaacg aacaggtcat cgacggcaag ggctggcgtt
     5281 ccggcgcggt ggtcgagaag cgcaagctga accagtggtt cctgcgcatc accgactatg
     5341 ccgacgacct gatcgaaggc ctcaaggatc tcgaccgctg gccggacaag gtccgcttga
     5401 tgcaggagaa ctggatcggt cggtccaagg gcctgcagat gacgtggaaa tgggccgggg
     5461 acgcgccccc ctccttgtcc ggggccgcct cgcctgacgg tctggaaatc tacaccaccc
     5521 gccccgacac cctgttcggc gcgtccttca tgggcatcgc gcctgaccat ccgatcgccc
     5581 gggccatggc cgaggctgat cccgccgtcg ccgagttcac cgcgaaatgc cgcgccggcg
     5641 ggacgtcgca ggccgacatc gaacaggccg agaagatcgg ctgggacacg gggctgaagg
     5701 tcacgcatcc gttcgacggc cgcgaagtca gcgtctggat cgccaacttc atcctgtcgg
     5761 aatacggcac gggcgccatc ttcggctgcc cggcccacga tcaacgcgac ctggattttg
     5821 cgcgcaaata cgacctgccg gtgatcccgg tggtcaagcc cgccgacgcc gccgacgact
     5881 tcgccgtcga gaatgaggcc tatgtcggcc cgggcgccct gttccattcc gacttcctcg
     5941 acggcatgtc gatcgaggac ggcaaggcgg cggccatcca gcgcatcgaa gacgcgggca
     6001 tgggcaaggg cgcgaccata tatcgcctgc gcgactgggg cgtgtcgcgc cagcgctact
     6061 ggggttgccc gatcccgatc atccactgcc cgtcgtgtgg cgttcagccg gttcccgccg
     6121 accagttgcc ggtcatcctg cccgacgatg tcaccttcga cgtgcccggc aatccgctcg
     6181 accggcaccc gacgtggaaa cacgtcaaat gcccggcctg caacgaggac gcgacgcgcg
     6241 agaccgacac gctcgacacc ttcgtagact ccagctggta tttcgcccgc ttcactgacc
     6301 cgacggccga cgccccaatc gacaaggcgg ccgccgatca ctggctggcg gtcgatcaat
     6361 atatcggcgg ggtcgagcac gcggtgctgc acctgt
//
LOCUS       DASVTM010000398         5780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_199256,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000398 DASVTM010000000
VERSION     DASVTM010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199256"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VF633_10920"
     CDS             <1..129
                     /locus_tag="VF633_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX8471616.1"
                     /translation="IYGMNFEHMPELPQVWGYPFALMLMLIAAVLPLLWFKRKGWL"
     gene            complement(223..954)
                     /locus_tag="VF633_10925"
     CDS             complement(223..954)
                     /locus_tag="VF633_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A-modifier LpxR family protein"
                     /protein_id="HEX8471617.1"
                     /translation="MSTAPEGREFGPVIDFQSDYPADRFSPAKERMSPFAATSRSDIW
                     REGEGYTDRLRLTAHGDLRRADGAPVPPTLQEQSDFRPDSYDVTYTRGWPAARGYTPS
                     GLEVSLTPHAGVGIGDQGGLAEAGATLKIGSDLARLVPRGSREFGERSRWYVYAASSG
                     RAVGYNFARNRDGDFTRSGVSQDRGTFLGDASIGVAMRRGDMQTSFGVVYREIKAKGM
                     RGGEGIDRDVSEGLVAFQFSIRPER"
     gene            complement(1303..3186)
                     /locus_tag="VF633_10930"
     CDS             complement(1303..3186)
                     /locus_tag="VF633_10930"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX8471618.1"
                     /translation="MAGLEAYKTTEFAVLSGEKDAARPQLSVVRSLELDRSRDDLLTD
                     FGKKTLEDRYLLAGESYQDMFARVSTAFSDDADHAQRVYDYMSKLWFMPSTPVLSNGG
                     ADRGLPISCFLNAVSDSLDGIQQVWNENVSLASNGGGIGTYWGGVRSIGEKVKGAGQT
                     SGIIPFIRVMDSLTLAISQGSLRRGSAAVYLDVHHPEIEEFLEIRKPSGDFNRKSLNL
                     HHGINITDEFMEAVRAGGSFGLRSPKNDEVLKVVDARSLWQKILEIRLQTGEPYLIFS
                     DTVNRQMPQHQKDLGLKVKQSNLCSEIMLHTGRDHLGIDRTAVCCLSSVNAEKFLEWR
                     EEPRFIEDIMRFLDNVLEDFITRAPPEMKAAVYSAKRERSVGLGLMGFHSFLQAQGVA
                     FESAMAKSWNMRLFKHLRREADKASVKLAEEKGACEDAAERGVMERFSHKLAIAPTAS
                     ISIICGGTSAGIEPIPANIYTHKTLSGSFAVKNPYLEELLETKGANTAVVWSSILEHE
                     GSVQHLDMLSDDEKGIYKTAFELDQRWVVELAADRSPEICQAQSINLFIPGDVNKWDL
                     HMLHWRAWETGVKSLYYLRSKSVQRAAFAGAEAKIVAEIDPNQPDLFSAARTDYDECL
                     ACQ"
     gene            3475..4242
                     /locus_tag="VF633_10935"
     CDS             3475..4242
                     /locus_tag="VF633_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8471619.1"
                     /translation="MSTPDVIPFARRAFALARTEIAALGALFVMAVGCLSFAELAEDI
                     REPDGMAFDARILHLLRPYADDPSRPWGPWWLKEATSDITSLGGISVLALFATIVIIF
                     LLIQRKWLSSALLVVGLGGGVMLSEGLKAVFERDRPPALYRATETINASFPSGHALLS
                     AVFYLSLGVMLTRAFPEKRFKAYVLGVGILITLLIGTTRIYLGAHWASDVFAGWSVGA
                     AWATALWLVSYVVSRRQRRHGAPLQDEATPDVGSRRA"
     gene            4753..5721
                     /locus_tag="VF633_10940"
     CDS             4753..5721
                     /locus_tag="VF633_10940"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX8471620.1"
                     /translation="MTSFAAFADYLDAQDAPEDLKIVVAAIAEECVAISRLVSLGALA
                     GALGVAGSVNVQDEEQKKLDVMTNDMLTDRLKACGPVAAVGSEEMDLIEPTGRQGGYL
                     VTFDPLDGSSNIDINAPVGTIFSILPAPAGQNPTEADFLIGGRKQVCAGYAVYGSQTM
                     LVLTLSEGVTGFTLDDQGVWRLTHPDVRVPADTREFAINMSNRRHWAEPVQRYIDGCL
                     AGKTGSRGKDFNMRWVAAMVADVHRILMRGGVFLYPWDQREPDRPGKLRLLYEGAPMA
                     LLIERAGGKATTDGVTPILDVIPTRLHDRVAVALGSANEVDAIAAA"
BASE COUNT         1017 a         1838 c         1838 g         1087 t
ORIGIN      
        1 atctacggca tgaatttcga acacatgccc gagttgccgc aggtctgggg ctatccattc
       61 gccttgatgc tcatgctcat cgccgccgtg ctcccgctgc tctggttcaa gcgcaaggga
      121 tggctatgaa ggcgagagac catgggttcg ggcttgggga ataggcgaag taagccagag
      181 tcgctgacgc atcgccccta acccgggtgc ctgctcctaa ccctatcgtt ccggtctgat
      241 cgagaactgg aacgccacca ggccctcgga cacgtcgcgg tcgatgcctt cgccaccgcg
      301 cattcccttg gctttgatct cccgataaac gacgccgaac gaggtttgca tgtcgcctcg
      361 ccgcatggcc acgccgatgg acgcatcacc gaggaaagtc ccccggtcct gactgacgcc
      421 tgagcgggtg aagtcgccgt cacgattacg agcgaaatta tagccgacgg cccgacccga
      481 gctggccgca taaacgtacc accgtgagcg ctcgccgaac tcgcgtgatc cccggggcac
      541 caggcgagcg aggtcggagc cgatcttcag cgtggctcca gcttcggcca gcccgccctg
      601 atcgcctatg cccactccgg cgtggggcgt caggctgact tccagaccgg agggcgtata
      661 gccgcgcgcc gcaggccagc cacgggtgta ggtgacgtca tagctatcgg gccggaaatc
      721 gctctgctcc tgtagcgtcg gcggtacggg agcgccatcc gcgcgcctca ggtccccatg
      781 ggccgtgagc cgcagacggt cggtgtagcc ttcgccttcc cgccagatat ccgagcggct
      841 tgtcgcggcg aaaggcgaca tacgctcctt ggcaggactg aacctgtccg ccgggtagtc
      901 actctggaaa tcgattacgg gaccaaactc acggccttcc ggggccgtgc tcacggcgta
      961 gctctgggag gttagactgg ccgtcgtcgc cggggtcggt gaggttgacg acgcacccac
     1021 gctctgacgg agggtctgtt cttcctgcgc gcgagcccat tcagcgttaa gagtcacctg
     1081 cgcggcggcc gaggtcgcga tgcccccgaa cgaagctatg ccgatgatga cccttccaaa
     1141 gcgcttcaac ggtcccctca cagtccgtta acgtccgcca ccctgactcg aaagtgggcg
     1201 gacgcctaga gactatttca caacacgatc ctgacacgcg ctgaatgcga gtttggacgg
     1261 tcaagttccc cggagaagag ggttaaggcc ggaaaccggg ccttactggc aggccagaca
     1321 ctcgtcgtag tcggtgcgag cagccgagaa gaggtccggc tgattgggat cgatctcggc
     1381 cacgatcttg gcctcggcgc cggcgaaggc cgcccgctga acggacttgg agcgcagata
     1441 gtagagtgac ttcacgccgg tctcccatgc gcgccagtgc agcatgtgca ggtcccactt
     1501 gttgacgtcg ccggggatga acaggttgat cgactgcgct tggcagattt ccggcgagcg
     1561 gtcggcagcc agttcgacaa cccagcgctg gtccagttcg aaggcggtct tgtagatgcc
     1621 cttctcgtcg tcgctcagca tatcgaggtg ctggaccgaa ccttcatgtt ccaggatcga
     1681 ggaccagacg acggcggtgt tggcgccctt ggtctccagc agttcttcca gatacgggtt
     1741 cttgacggcg aacgagcccg acagggtctt gtgagtatag atgttggccg ggatcggctc
     1801 gatgcctgcc gaggtgccgc cgcagatgat cgagatcgag gcggtcgggg cgatggccag
     1861 cttgtgcgag aaccgctcca tgacgccgcg ctcggcagca tcttcgcagg cgcccttttc
     1921 ctcggccagt ttgacgctgg ccttatcggc ctcgcgacgc aggtgtttga acaggcgcat
     1981 gttccacgat ttcgccatgg cgctttcgaa cgctacgccc tgcgcctgca ggaacgagtg
     2041 gaagcccatc aggccgagac cgaccgaccg ctcgcgcttg gccgaataga cggcggcctt
     2101 catttcgggc ggcgcgcgcg tgatgaagtc ttccagcacg ttgtcgagga aacgcatgat
     2161 gtcctcgatg aagcggggct cttcgcgcca ctccaggaat ttctcggcgt tgaccgacga
     2221 caggcagcag acggcggtgc gatcgatacc gaggtggtcg cgtccggtgt gcagcatgat
     2281 ttccgagcac agattggact gcttgacctt gaggccgagg tccttctggt gctgcggcat
     2341 ctgccggttc accgtgtcgg agaagatcag atagggctcg ccagtctgca ggcggatctc
     2401 gaggatcttc tgccacagcg aacgggcgtc gacgaccttg agcacttcgt cgttcttggg
     2461 cgaacgcagg ccgaacgaac cgccggcgcg gactgcttcc atgaactcat cggtgatgtt
     2521 gatgccgtgg tgcaggttca gggacttgcg gttgaagtcg cccgacggct tgcggatttc
     2581 gaggaactcc tcgatctcgg ggtggtgcac gtccagatag acggcggccg aaccgcgacg
     2641 cagcgaacct tgcgaaatcg cgagtgtcag ggaatccatg acgcggatga aggggatgat
     2701 gccggaggtc tggcctgcgc ccttgacctt ctcgccgatc gaccggacgc cgccccagta
     2761 ggtgccgatg ccgccgccgt tcgaggccag cgagacgttc tcgttccaga cctgctggat
     2821 gccatccaac gaatcgctga ccgcgttcag gaagcatgaa atcggcaggc cgcgatcggc
     2881 gccgccgttc gacagcaccg gcgtcgaggg catgaaccag agcttggaca tgtagtcgta
     2941 gacgcgctgg gcgtgatcgg cgtcgtcaga gaaggcggtc gagacgcggg cgaacatgtc
     3001 ctgatagctc tcgcccgcca gcaggtaacg atcctccagc gtcttcttgc cgaagtcagt
     3061 cagcaggtcg tctcgcgaac ggtccagttc cagcgagcga accacggaca actgcggccg
     3121 cgccgcatct ttttcgccgg aaagcacagc aaattccgtg gtcttgtatg cctcgaggcc
     3181 agccatgatc gtcaacgtcc tgaacaaaaa gcgcgattga agctcccgcg tccggacaca
     3241 tcatctagtg gccgaagcgc tccccgagcc cacatatagg gcgcagatgc ctaacgaccc
     3301 gtcaacggct tgactccggg ttcgtggccg aatcagacga aagacagggg gcagtcctgt
     3361 gggaaaccgc gcagaggccc gccacagggc tgtgagggca agtgcgctcg ggaatcacga
     3421 acgcgtgaaa cgccccgtcc cggaacgcga tgggagcgtg accgttcacg gacgatgtcg
     3481 acacctgatg tgatcccgtt cgcgcgccgc gccttcgctc tagcgcgcac cgagatcgcg
     3541 gcgcttggcg ccctttttgt catggcggtc ggctgtctca gcttcgccga acttgccgag
     3601 gacatacgcg aaccggacgg catggctttc gacgcacgca ttctacacct ccttcggccc
     3661 tacgccgatg atccctcccg gccctggggc ccgtggtggc tgaaggaagc gacgtcagac
     3721 atcacttcgc tcggcggcat ctcggtcctt gccctgtttg cgaccatcgt catcatcttc
     3781 ctgttgatcc agagaaaatg gctctcctcg gccctgttgg tggtcggtct cggcggtggg
     3841 gtgatgctga gcgaaggctt gaaagccgtg ttcgaacggg accgcccgcc ggccctctat
     3901 cgggcgaccg agacgattaa cgccagcttc ccgtccggcc acgcccttct gtcggcggtc
     3961 ttctatttgt cgcttggggt catgctgacc cgcgccttcc cggagaagcg cttcaaggcc
     4021 tacgtgctcg gtgtcggaat cctgatcacc ttgctgatcg ggacgacgcg catctacctt
     4081 ggagcccact gggcatcgga cgtcttcgcg ggctggagcg tcggcgcggc atgggccacg
     4141 gcgctgtggc tggtctccta cgttgtctcc cgccgtcagc gccgccacgg cgcgccgctt
     4201 caggacgagg cgacgccgga cgtcggctca cgcagggcat gaagtccggt caacggcccg
     4261 ctggtatcga tgggcaaagc ggccagcaac gcccaacgct ggatagatga cgtcaccgtc
     4321 acgtgcggcg aacatgtagg tagctggttt cgcgtcgatc ctggtcaggc cgaccccgcc
     4381 gtatcgctcc tgatgtcacc cgttgccgaa ccccgtcttg ccggtgtcgg cgcccagttc
     4441 cggcagcgca gcatccatgc ctcgccttgg cgcagatagg tttccctcgc agtgaggttt
     4501 tcttccgcga tagcgcgttc gaagcagcgt cgggcgcgtt cacgacactg ggcggcggtg
     4561 acaggagaag cttccatggc gtctgtggcc tgcgtccgtt ggaatgcacg gatgccttgt
     4621 gtaaccaggt cgacacagga ttggttcctg tgcggccctg gacagatggc caactgcttg
     4681 cagcgccgct tggcggttcc cctcgcgacc ggtttcggga taggaaagcc gcagacaaaa
     4741 cgggattctt gaatgacgtc gttcgccgcc ttcgccgact acctcgacgc acaggacgcg
     4801 cccgaggatt tgaagatcgt ggtagccgcg attgctgagg aatgcgtcgc catctcccgg
     4861 ctggtctcgc tcggggccct cgccggagca ctcggcgttg cgggaagcgt caatgtccag
     4921 gacgaggagc agaagaagct cgacgtcatg accaatgaca tgctgaccga ccggctcaag
     4981 gcctgcggac ccgttgcagc cgtcgggtcg gaggagatgg acctgatcga gcccaccggt
     5041 cgtcagggcg gctatctggt cactttcgat ccgctcgacg gctcgtccaa tatcgacatc
     5101 aacgcccccg tgggcaccat cttctcgatc ctgccagccc ccgcgggtca gaatccgacc
     5161 gaggccgact tcctgatcgg cggccgcaag caggtctgcg ccgggtatgc ggtctatgga
     5221 tcgcagacca tgctggtcct gaccttgtcc gagggcgtga ccggcttcac cctcgacgat
     5281 cagggcgtct ggcgcctgac ccatcccgac gtccgcgtgc ctgcggacac cagggagttc
     5341 gcgatcaata tgtcgaaccg ccgtcattgg gccgagcctg tgcagcgcta catcgacggc
     5401 tgtctggcgg gaaagaccgg atcgcgcggt aaggatttca acatgcgctg ggttgcggcc
     5461 atggtcgccg acgttcaccg catcctgatg cgcggcggcg tcttcctcta tccttgggac
     5521 cagcgcgagc ccgaccgtcc cggcaagctg cgtctgctgt atgaaggcgc cccgatggcg
     5581 ctgctgatcg agcgggcagg tggcaaggcc accaccgacg gcgtgacccc catcctcgac
     5641 gtcatcccga ccaggctcca cgaccgcgtc gccgtggctc tcggttcggc caatgaagtc
     5701 gacgccatcg ctgccgcctg acgttagggg ctagacctcg aagacccggt aaggcttttc
     5761 gccaagcgtc cgcagaaccg
//
LOCUS       DASVTM010000399         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_732003,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000399 DASVTM010000000
VERSION     DASVTM010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732003"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VF633_10945"
     CDS             complement(<1..896)
                     /locus_tag="VF633_10945"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX8471621.1"
                     /translation="MNIQGWAEIALTLALTIGLGWPIGVYMSRVWNGERTWLDPVLKP
                     FEAFFYTAAGVDPKRSQSWLGFAGALLAFNAVGFFLLFAILRLQGVLPLNPQGFAGMS
                     PHLAFNTAVSFVTNTNWQSYGGESTASTFSQMVGFTTQNFVSAATGATIAAALARAFV
                     ANRGEGVGNFWADLTRTTLYVLLPLAFVVAVTLTGLGVVQSLAASATATGLEGASQTI
                     SLFPAASQEAIKQLGINGGGIFNANSAHPFENPSPLTNLIEAVAIGVMGWASFFAFGR
                     SVLAKKDIRALTLAATVLISFSA"
     gene            complement(909..998)
                     /locus_tag="VF633_10950"
     CDS             complement(909..998)
                     /locus_tag="VF633_10950"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HEX8471622.1"
                     /translation="MTMIAILWGVGAVVVAVYMIAALVRPERF"
     gene            complement(1789..>2326)
                     /locus_tag="VF633_10955"
     CDS             complement(1789..>2326)
                     /locus_tag="VF633_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471623.1"
                     /translation="QGDVVSLRGGALIGRSAFICGDPYSAADVFEKAVAQRPTVEARF
                     NLATAYARTARYQAAADLYRTVVANGQFTVLTLDTEQGVSGSRPASFNAADEAAVRLA
                     DMGARIAADQSTAAIGDTGEDATNPIQAIIDRAHVPSDRALFFDGLTTDPELTAVPSP
                     VTHSRWRQCTGVGRIGIV"
BASE COUNT          441 a          708 c          771 g          406 t
ORIGIN      
        1 gccgagaacg agatcaggac cgtggcggcc aatgtcagag cgcgaatgtc cttcttggcc
       61 agcacgctgc ggccgaaggc gaagaacgag gcccagccca tcacgccgat cgctacggcc
      121 tcgatgaggt tggtcagggg cgaggggttc tcgaacgggt gagcggagtt ggcgttgaag
      181 atgccgccgc cgttgatgcc cagttgcttg atggcttcct ggcttgcggc cgggaacagg
      241 gaaatcgtct gcgatgcgcc ttcgagaccg gtcgccgtgg ccgatgctgc caggctttgc
      301 acgacgccga gaccggtcaa ggtgacggct acgacgaagg ccagaggcag caggacgtaa
      361 agcgtggtgc gcgtcaggtc ggcccagaaa ttgccgacgc cttcgccgcg attcgcgacg
      421 aacgcccgtg ctagagcggc tgcgatcgtc gcgcctgtcg ccgcagaaac gaagttctgg
      481 gtggtgaagc cgaccatctg gctgaaggtc gatgcggtgc tttcgccgcc gtacgactgc
      541 cagttggtgt tggtcacgaa gctgacggcg gtgttgaagg ccagatgggg tgacatgccc
      601 gcgaagccct gcgggttcag cggcagtacg ccctgtagac gcaggatggc gaacagcagg
      661 aagaagccga cggcgttgaa cgccagcagc gcaccggcga atccgagcca gctctggctg
      721 cgtttcggat cgacgccggc ggcggtgtag aagaacgcct caaagggctt gagcaccggg
      781 tcgagccagg tgcgttcgcc gttccagacg cgagacatgt agacgccgat gggccagccg
      841 aggccgatcg tcagcgcgag tgtgagggcg atttccgccc aaccttgaat gttcatgatg
      901 gcggctccct agaaccgctc ggggcgcacg agcgccgcga tcatgtagac ggcgacgacg
      961 acagcgccga ctccccagag gatggcgatc atcgtcacac cctcttcagg gcagcggcca
     1021 gtgcggcgaa gacgagaaac gccccaccac ccagcaccag gaaaaacaga tcggccattg
     1081 tcggcgcctc caataatcag gaggccggtg gaccacgaag ccgcataacg ggaccattcg
     1141 agtctggtcc ctgcgcataa cgtggacgta aaattcagga cgtattccag gtgcggcaac
     1201 acaggaaaaa gccgggcatc tcaggcgcca ctgggttccc ttcggcgacg tctatgatgg
     1261 cgggagggac catccggcaa agtatcaggg ccaaggcttg ggcctcagct cccgtgcgag
     1321 ttcgctcagg catgttggcg cacctttggt ttcggaatgc ccgctagagc atccgcctga
     1381 cgctgaagcg tcctggcgat cctttctgcc gcccggcgac tgcggaaggg ccgggtgcag
     1441 gcgccgttga cgctgatgct ccagccgcga gtccatgcga ccacttcgta gtgcgcgcac
     1501 tcggcgcccg ggactgcgag ggcgtgtcga cacaatccga catcatcgca acgccctcct
     1561 caggtcacgg tgccccgttc cgacgttctt gaggatccgg tgcccaaggc gcggtcaagc
     1621 ccggtcatcg tcatcgccca tacgcccgac gggggaggct cctcgagggc gacgttgcgg
     1681 tggcggttca acaccgtcgc acccgccggt tgttccgtgg ggaacccggg aatcgcttta
     1741 tccgcaggcg ggtcgtctca gggccttcaa cattgatagc cgagccactt acacgattcc
     1801 gatccgccca acgccggtgc actgcctcca acggctatgg gtgactggcg acggcacggc
     1861 cgtgagttcc gggtctgtgg ttagaccgtc aaaaaacagg gcgcgatcag aggggacgtg
     1921 ggctcggtcg atgatggcct ggattggatt tgtcgcatct tcgcccgtat cgccgatcgc
     1981 ggccgtgctt tgatcggccg cgatcctggc tcccatgtcg gcaagccgca cggctgcctc
     2041 gtcagctgca ttgaaactcg caggacggga accgctgact ccctgttctg tatcgagcgt
     2101 caagacggtg aactggccgt ttgcaactac cgtgcgataa aggtcagctg ccgcctgata
     2161 tcgagccgtt ctggcgtacg cggtggcaag attgaaccgt gcttcaacgg ttggccgctg
     2221 ggctacagcc ttctcgaaaa cgtcggctgc cgaatagggg tcgccacaga tgaatgctga
     2281 ccgtcctatg agggcgcctc cgcgtagact gacaacatcg ccctgg
//
LOCUS       DASVTM010000400         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_837673,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000400 DASVTM010000000
VERSION     DASVTM010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837673"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VF633_10960"
     CDS             <1..194
                     /locus_tag="VF633_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX8471624.1"
                     /translation="FEGLEIPEVLLSGDHRKMAAWRQEQRELTTQERRPDLWAAHLQT
                     LPAESSAKSKLKGAKARGE"
     gene            199..624
                     /gene="rplS"
                     /locus_tag="VF633_10965"
     CDS             199..624
                     /gene="rplS"
                     /locus_tag="VF633_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX8471625.1"
                     /translation="MANILQTIEAEEKARLVAIREIPNFQPGDTLRVMVKIKEGERER
                     IQAFEGVCIARDGGGVNESFTVRKISFGEGVERKFPILSPNIDAIEVKRRGVVRRAKL
                     YYLRDRRGKSARIAERQTVRPVKGAVVPVTGTSDKSDEA"
     gene            complement(728..1207)
                     /locus_tag="VF633_10970"
     CDS             complement(728..1207)
                     /locus_tag="VF633_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEX8471626.1"
                     /translation="MPNRHDIHVLNSLVEATLDSAERLEQTASESDASGHGSLFSSLA
                     AERRDIARVLSESVTALGGQPNTRGSILAKAQRAVKDMSHALMGNEAGLIDSADQGEA
                     ALDKKYEAAIADDRLSATTRETLRRAHAAIHREAHDLHDLRRSVDGQRDASSNLFPQ"
     gene            complement(1300..2109)
                     /locus_tag="VF633_10975"
     CDS             complement(1300..2109)
                     /locus_tag="VF633_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEX8471627.1"
                     /translation="MTDAPSTPSTMTYARYLGLDQLLSAQDPISDQHDEMLFVIIHQT
                     KELWLKQILHEVALAQALVRNGDLVPAYKSLARVSRIQSVMTQSWDILATMTPADYLR
                     FRNVLGSSSGFQSDQFRRFEAMLGLKDAKFLSFQEDRPEAYAALKSAIEAPSLYDDAL
                     AQLARAGLPIPDDVLNRDVSQPWQPSEGVEAAWLEVYRDTDRRWAQYQLAEKLVDLDD
                     ALLTWRHKHVVTIERIIGRRRGTGGTEGVAYLTTTLEKRCFPELWSLRTKL"
     gene            complement(2140..2946)
                     /locus_tag="VF633_10980"
     CDS             complement(2140..2946)
                     /locus_tag="VF633_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX8471628.1"
                     /translation="MRQALSALLLLLGACASQIAALPASAEVPVRGYKIVRTFPHGTD
                     AFTEGLFYRGEGADAELVESTGRFPSDIRIIRLADGEVLKKRVLDTAYFGEGIVQVGD
                     RLISLTWRNHIGFIWNATDLTPISAFSYTGEGWALTKDDHRIIMSDGTPELRFLDPQT
                     LQETGRLLVTADGEPVDELNELEWINDADGPHIYANIWQTDRIAKIDPATGHVVAWID
                     LTGLFPVSERKDPTDDVLNGIAWDAAGKRLFVTGKNWPQLFEIRLTDTPH"
     gene            complement(2954..>3470)
                     /locus_tag="VF633_10985"
     CDS             complement(2954..>3470)
                     /locus_tag="VF633_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX8471629.1"
                     /translation="GGLFLLWKATKEIHHSVDPTPSHDEVLDKKSAVINNAGAAIIQI
                     ILLDLVFSVDSILTAVGMTDHVPIMMVAVIIAVTVMLLASDPLANFIDKNPTIVMLAL
                     GFLLMIGAVLIADGFGVHVPKGYIYAAMAFSGLVEGLNMASRNSAQKKQALLDAHQTE
                     LNKRETAEPGV"
BASE COUNT          638 a         1066 c         1102 g          664 t
ORIGIN      
        1 cgttcgaggg gctggagatc cccgaggttc ttttgtcggg cgaccatcgc aagatggcgg
       61 cctggcgtca ggaacagcgg gaactgacga cgcaggagcg acggcccgat ctgtgggccg
      121 ctcacctgca aacactacct gcggaatctt ccgccaaatc aaagctaaag ggcgcaaaag
      181 cccgaggaga atagacacat ggccaatatc cttcagacca tcgaagccga agaaaaagcc
      241 cgcctggttg cgatccgcga aatcccgaac ttccagcctg gcgacacttt gcgcgtcatg
      301 gtgaagatca aggaaggcga acgcgaacgt attcaggcct tcgagggcgt ctgcatcgcc
      361 cgcgacggcg gcggcgtgaa cgagagcttc actgtccgca agatttcctt cggtgaaggc
      421 gtcgaacgta aattccctat cctttcgcca aacatcgacg cgatcgaagt caagcgtcgc
      481 ggcgtcgttc ggcgcgccaa gctgtactac ctgcgcgacc gtcgcggtaa gtcggcccgt
      541 atcgccgagc gccagacggt tcgtccggtc aagggcgccg tggttccggt caccggcacc
      601 tccgacaagt ccgacgaagc ataggcaatc tttttgcgct accgcccttc gggctacttg
      661 agcgcagtta aatgacgaag gccccggtcg cgagaccggg gcctttttca ttcaggctga
      721 ctcgcttcta ttgcgggaac aggttcgagc tcgcgtccct ctggccgtca acgctgcgac
      781 gcagatcgtg cagatcatgg gcctcacggt ggatcgcggc gtgagctcgg cggagcgttt
      841 cgcgcgtcgt ggcggacagt cggtcatcgg cgatggcagc ctcatacttc ttgtccagcg
      901 ccgcctcacc ctgatcggcg ctatcgatca aacctgcctc gttgcccatg agcgcatggc
      961 tcatgtcctt cactgcccgc tgtgccttgg cgaggatcga tcctctcgtg ttgggctgac
     1021 ctccgagggc ggtgacactc tccgacaaga cccgggcgat gtcgcggcgc tcggcagcca
     1081 atgagctgaa cagggatcca tgacctgaag cgtcgctttc acttgcggtc tgctcaagcc
     1141 gttcggcgct gtcgagggtc gcctcgacga ggctgttcag aacgtgaata tcgtggcggt
     1201 taggcatgag ggactccaat cttcatatat ataacggcca cgctcttccc gagttgcggc
     1261 atcaagactg ccgatcgatc actgtcacgt cagccgcagt cacagcttcg tgcggagcga
     1321 ccagagttcg gggaagcagc gcttctcaag cgtcgtcgtc aaataagcga cgccttcggt
     1381 gccgcctgta ccccggcgcc ggccgatgat gcgctcaatc gtgaccacat gtttatggcg
     1441 ccaggtcagc agggcgtcgt ccagatcgac cagcttctcg gccaactggt actgcgccca
     1501 ccggcggtcg gtatcgcgat agacctccag ccacgctgcc tcgacacctt cggacggctg
     1561 ccagggctgg ctgacgtcgc ggttcagaac gtcatccggg atcggcaacc ccgccctggc
     1621 cagttgagcg agagcgtcgt catagaggct gggcgcctcg atcgccgact ttagagccgc
     1681 ataggcttcg gggcggtctt cctgaaagct caggaacttg gcgtccttga gacccagcat
     1741 ggcttcgaaa cggcggaact gatcactctg gaagccggag ctcgacccca gcacgttgcg
     1801 gaaacgcaga taatccgcag gcgtcatggt ggcgaggatg tcccagctct gggtcatcac
     1861 ggactggatt cgactgaccc gcgcgaggct cttgtaggcc ggcaccagat cgccgttgcg
     1921 caccagagcc tgcgccagcg cgacctcgtg caggatctgc ttgagccaca gttccttggt
     1981 ctggtggatg atgacgaaca gcatctcgtc gtgctgatcg ctgatcgggt cctgggccga
     2041 cagcagctgg tccagcccga gatagcgggc gtaggtcatg gtcgaggggg tcgaaggtgc
     2101 gtcggtcatg cctgtctatc gccctgatct gcgacaggct caatgcggcg tgtcggtcag
     2161 cctgatctcg aacagctgcg gccagttctt gccggtgacg aacagccgct tgcccgccgc
     2221 gtcccaggcg atgccgttga ggacgtcgtc ggtgggatcc ttgcgctcgg acaccggaaa
     2281 aaggccggtc aggtcgatcc acgccacgac atgcccggtg gcggggtcga tcttggcgat
     2341 ccggtcggtc tgccagatat tggcgtagat gtgagggccg tcggcgtcgt tgatccattc
     2401 gagctcgttc agctcatcga caggctcgcc atccgcggtg accagcaacc gtccggtctc
     2461 ctgcagggtc tgggggtcga gaaagcgcag ctctggcgtg ccgtcgctca tgatgatgcg
     2521 gtggtcgtcc ttggtcaggg cccagccctc gcccgtatag ctgaaggccg aaatcggcgt
     2581 caggtcggtc gcgttccaga tgaagccgat atggttgcgc cacgtcaggc tgatcagccg
     2641 gtctccgacc tgaacgatgc cctcaccgaa ataggcggta tcgagcaccc gcttcttcag
     2701 cacctcgccg tcagccaggc ggatgatgcg gatgtcggac gggaaacgtc ccgtgctctc
     2761 gaccagttcg gcgtcggcgc cctcaccgcg atagaacagg ccttcggtga aggcgtctgt
     2821 tccatgcgga aaggtccgga cgatcttgta gccccgaacc ggaacctcag cggacgccgg
     2881 cagggccgcg atctgcgacg cgcaggctcc gaggagcagc aggagggcag agagggcttg
     2941 gcgcatcctt cgcctatacc ccgggctccg ccgtttcgcg cttgttcaat tcggtttggt
     3001 gagcatccag cagcgcctgc ttcttctgcg ccgagttccg gctcgccatg ttgagcccct
     3061 cgaccagacc cgagaaggcc atggcggcgt agatatagcc cttgggcaca tggacgccga
     3121 agccgtcggc gatcagcacg gcgccgatca tcagcaggaa gccaagggcc agcatgacga
     3181 tggtcgggtt cttgtcgatg aagttcgcga gtgggtccga agccagcaac atcaccgtca
     3241 cggcgatgat gaccgcgacc atcatgatcg gcacgtggtc ggtcatgccc acggcggtca
     3301 ggatcgagtc gaccgagaag accaggtcga gcaggatgat ctggatgatg gcggcgccgg
     3361 cgttgttgat gacggccgac ttcttgtcga gaacttcgtc atggctcggg gtgggatcga
     3421 ccgagtggtg gatttccttg gtcgccttcc acagcaggaa caggccgcct
//
LOCUS       DASVTM010000401         8197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1049226,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000401 DASVTM010000000
VERSION     DASVTM010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049226"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..717
                     /gene="rplM"
                     /locus_tag="VF633_10990"
     CDS             241..717
                     /gene="rplM"
                     /locus_tag="VF633_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX8471630.1"
                     /translation="MLKSTTAALKPSEVERKWIHIDAEGVVVGRLATFIAMRLRGKHL
                     PTYTPHVDSGDYIVVTNVDKVVFTGKKLTDKIYYRHTGHPGGVKSTTPEKVLNGRFPE
                     RVLEKAVERMMPKESPLARGQMTHLRLFAGSAHEHDAQQPETIDFAGRSAKNTRSI"
     gene            720..1211
                     /gene="rpsI"
                     /locus_tag="VF633_10995"
     CDS             720..1211
                     /gene="rpsI"
                     /locus_tag="VF633_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX8471631.1"
                     /translation="MTDVTQEQATGFDALRSLGGSSAEDAPVYVQKLDAQGRAYSTGK
                     RKNAIARVWIKPGTGKFTINGKDQLQYFAREILRMMIAQPLEVTDRATQFDVDCTVQG
                     SGLSGQAGAIRHGLSHALTHYEPALRPVLKPHGFLTRDSRVVERKKYGRAKARRSFQF
                     SKR"
     gene            1446..2390
                     /gene="argC"
                     /locus_tag="VF633_11000"
     CDS             1446..2390
                     /gene="argC"
                     /locus_tag="VF633_11000"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX8471632.1"
                     /translation="MTHTLFIDGEAGTTGLEIRQRLEGRADLEFISLTGDRRKDPAAR
                     REALNAADAVILCLPDEAAKEAVAMIDNTTTRVIDASTAFRVAEGWTYGFAELDRNQR
                     AAIAASTRVSNPGCYPTGFIGLMRPLVSAGIVPTDAFVTVNAVSGYSGGGKSMIAEFE
                     GADKDKAPAYRTYGLSLHHKHVPEMTRHSGLSNEVLFAPAVGTYRQGMLVEIPLQLSA
                     LPSSTSIEVIHGALVEAYAGQQFIEVADLETTEAMTGIDPEGLNGTNRMRLHVFGDRH
                     GHQARLVALLDNLGKGASGAAVQNLNLMLGLDEATGLT"
     gene            2431..2934
                     /gene="msrB"
                     /locus_tag="VF633_11005"
     CDS             2431..2934
                     /gene="msrB"
                     /locus_tag="VF633_11005"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8471633.1"
                     /translation="MTQAILIPRRALLMGAGALSIAACSSGKASAAADPFASSPIRRT
                     TDAQWRTRLPGLSYDVLRHEATERPYSSPLNEEHRAGTFVCKACDLPLFLSAWKFESG
                     TGWPSFFRANPANIGTKRDLAIGFPRTEYHCARCLGHQGHLFDDGPAPTGKRYCNNGV
                     ALHFRPG"
     gene            complement(2939..3316)
                     /locus_tag="VF633_11010"
     CDS             complement(2939..3316)
                     /locus_tag="VF633_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471634.1"
                     /translation="MKRLEPNFLLAVSTGVALALLILTAVVFGQPGGEIKYPLMAAIC
                     VIAYVIGNTLMQRWMKRVTPPMVHVGMPATAALSSIFPLVVILFAAVPVFWSGRDYGL
                     FIIIAAVMTGATIESAMKARNPG"
     gene            complement(3313..5076)
                     /gene="phaC"
                     /locus_tag="VF633_11015"
     CDS             complement(3313..5076)
                     /gene="phaC"
                     /locus_tag="VF633_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX8471635.1"
                     /translation="MLETLSMNIAKAAMTAQSAIAEAAMSQAANPRKEGQAALSPDPF
                     NVGPAMTSVMSSLAARPDKLFSAQADLFSRYMDLWSSTTRRASGETTQPPSKDKRFKD
                     AAWSENPMFDMMRQSYLVTSDWMNGLIASVEDVDPLTKRRAEFFTKLLTDAFSPSNFL
                     ASNPAALKAMADSNGESLVKGMQNFAADLERGAGKLAISQADYGKFVVGENVATAPGQ
                     VVWRDELFELIQFDAATDKQRAIPLLIFPPWINKFYIMDLQPENSLIRWLSAQGFTVF
                     VCSWVNPDVDKAAFGFDDYLEKGIYLAIEKALEQSQSETLNTVGYCIGGTLMGAALAH
                     MAANDDTRVGANTFFAAQHDFEEAGDLLLFTDEHWLAQVEEQMDAAGGVLPGAAMAET
                     FNALRANDLIWSFFVSNYLMGKSPAAFDLLFWNADQTRMPKALHMDYLRSMYGQNLLS
                     KGQFNIGGETVDLSKVTIPLYFQASREDHIAPMNSVYRSAQAFRNADVTLTLAGSGHI
                     AGVINPPSANKYQHWVNDALPATLAEWQANAEEHPGSWWTHWAEWLHERSGDWVPARD
                     PAKGPLKAIEPAPGSYVKVKS"
     gene            5484..5927
                     /locus_tag="VF633_11020"
     CDS             5484..5927
                     /locus_tag="VF633_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471636.1"
                     /translation="MTKKMMRPMFTVVTVVGLTCSLGACAGGFNPETDASSPVAPRVQ
                     ALVDANRVYPQWKDFPRSSDPVPQPAEIAQQVNTLRATGGALAGEVSALQWQNADSAA
                     FEREVAARVAATPIAPATAQTAAEIEAYAQTLRNRAKAPPPISRQ"
     gene            6008..7018
                     /gene="glpX"
                     /locus_tag="VF633_11025"
     CDS             6008..7018
                     /gene="glpX"
                     /locus_tag="VF633_11025"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX8471637.1"
                     /translation="MAPFDTQRLDQSRPGARPVPQTGFSFSSLAADAVSVTEAAAIAS
                     FAQIGRGDEQQADQLAVDAMRTGLNRLEIDGLIVIGEGERDEAPMLYIGEAVGTGRGP
                     AVDIALDPLEGTTLTAKAMPGALCVLSMSAGGGMLHAPDTYMDKIAIGPGYAPGTVDL
                     DMSPAENVRSLASAKGVDPRDITVCVMDRPRHSALIAALREVGAKVMLIGDGDVAGVI
                     HTADPDTGIDLYLGSGGAPEGVLAASALKCVGGHFQGRLVFRNDHERGRAHRIGITDL
                     NRKYDLHELVSTDAVFAATGVTSGALLDGVKIERGMISTHTVVMDSSTCTVRRIRTTR
                     PL"
     gene            7135..>8197
                     /locus_tag="VF633_11030"
     CDS             7135..>8197
                     /locus_tag="VF633_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX8471638.1"
                     /translation="MQALGLQEPLARALASRGVRADQGADFLTPTLKALFPDPSSFMD
                     MDAASTAILDAMTAGSSIHIFADYDVDGASSAALLVRWFRAMGSELPIYVPDRLTEGY
                     GPSAKAFDTLKALGADLVITVDCGAAANEAVAHAGDIGLNVVVIDHHMMREEPPKCLA
                     VVNPNRPGCNSGQGNLAAAGVVFVLLAALNREARARGLFADREQPDIRQWLDLAAMGA
                     ICDVTGLTGFNRALTGLGLRVMSDWKNPGLKALLSAAKAEEGTAKSNHAGFILGPRIN
                     AGGRIGKSDLGARLLSTDDPEEARAIAEELDALNIGRREIERLVTEEATRRAEATRAH
                     DDGSAMVVIAGDDWHPGVVG"
BASE COUNT         1445 a         2758 c         2568 g         1426 t
ORIGIN      
        1 cgggtgcttt tcctgcgccg tcagccagcc tgaacgccct aaagcatatc aaagcggctc
       61 atttcgtacg gtattttaat accgttattg aaaaatcgtt gcaaaacatg gcactagagc
      121 ctgttcggac attctacgcg cattgacgca tccgaaccca tcctctatca gcgcgccttc
      181 cgctcattcc cgcatccgca ggactgggcc catcgttcgt ctatcccagg aacctctgaa
      241 atgctgaaga gcactacggc tgccttgaag ccgtccgagg tcgagagaaa atggatccat
      301 atcgacgctg aaggcgtcgt cgtgggccgc ctcgcgacct tcatcgccat gcgtctgcgt
      361 ggcaagcacc tgccgaccta caccccccac gtggactcgg gcgactacat cgtcgtcacc
      421 aacgtcgaca aggtcgtgtt caccggcaag aagctgaccg acaagatcta ctatcgtcac
      481 accggccacc cgggcggcgt caagtcgacc acccctgaga aggtcctgaa cggccgcttc
      541 ccggaacgcg ttctggaaaa agccgtcgag cgcatgatgc cgaaggaaag cccgctggct
      601 cgtggtcaga tgacccacct gcgcctgttc gccggttcgg cgcacgagca cgacgcccag
      661 cagcctgaaa ccatcgactt cgccggccgc tcggccaaga acacccggag catctgagca
      721 tgaccgacgt cactcaagaa caagcgaccg gcttcgacgc cctgcgcagc ctcggtggtt
      781 cgtccgccga agacgcaccg gtctatgtcc agaagctgga cgcgcaaggc cgcgcctatt
      841 cgaccggcaa gcgcaagaac gcgatcgccc gcgtctggat caagccaggc accggcaagt
      901 tcaccatcaa cggcaaggat caactgcagt atttcgctcg cgaaatcctg cgcatgatga
      961 tcgcacagcc gctggaagtg accgatcgcg ccacccagtt cgacgtcgat tgcaccgttc
     1021 agggttcggg cctgtccggc caggccggcg ccattcgcca cggcctgtcg cacgccctga
     1081 cgcactacga accggccctg cgcccggtcc tgaagccaca cggcttcctg acccgcgaca
     1141 gccgcgtcgt cgagcgtaag aagtacggtc gcgccaaggc tcgccgttcg ttccagttct
     1201 cgaagcgcta agccgtttca ctacggtttg gatcagggcg cttcgggaaa ccggagcgcc
     1261 ctttttcttg tgctgtcctt ctccccttgc gggagaaggt ggcaggcgga gcctgacgga
     1321 tgaggggtca caccgctctg tccgttcgta atcaaagtaa gacgttacgg gtcagcgtga
     1381 cccctcatcc gacccgcagg gcgggccacc ttctcccgca gggggagaag gatcaggata
     1441 gtgaaatgac ccacaccctc ttcatcgacg gcgaggccgg cacgacgggc cttgaaattc
     1501 ggcagcggct ggaaggccgc gcggatctgg agttcatctc cctgaccggt gaccgtcgca
     1561 aggacccggc ggcacgccgc gaggcgttga acgctgcgga tgcggtgatc ctgtgcttgc
     1621 cggacgaggc ggcgaaggaa gccgtggcca tgatcgacaa cacgacgacc cgggtcatcg
     1681 acgcctcgac tgccttccgc gtcgccgaag gctggaccta cggtttcgcc gagttggacc
     1741 ggaaccagcg tgcagccatc gcggcctcga cgcgggtgtc gaacccgggc tgctatccga
     1801 ccggcttcat cggtctgatg cgcccgctgg tcagcgccgg catcgtgccc accgacgcct
     1861 tcgtgaccgt caacgccgtg tccggctact ccggcggcgg caagtcgatg atcgctgagt
     1921 tcgaaggcgc cgataaggac aaggccccgg cctaccgcac ctacggtctc agcctacacc
     1981 acaagcatgt gccggagatg acgcggcact cgggtctgtc gaacgaggtc ctgttcgccc
     2041 ccgccgtcgg gacctatcgt cagggcatgt tggtcgagat tcccctgcaa ctttcagccc
     2101 tgccgtcatc gacctcgatc gaggtcatcc atggcgctct ggtcgaggcc tatgcgggcc
     2161 aacagttcat cgaggtcgcc gatctcgaaa ccactgaggc catgaccggc attgatcccg
     2221 aaggcctgaa cggcaccaac aggatgcgtc tgcacgtgtt cggtgaccgt catggtcatc
     2281 aggcccggct ggtcgctctg ctggataatc ttggaaaagg cgcgtcaggc gcggcggtgc
     2341 aaaatctcaa cctgatgctg ggtctggatg aggcgacggg gctgacctga agccttgaaa
     2401 cctttcgcgc ttcggcgtcg tacttcaagc atgacccaag ccatcctgat cccacgccgt
     2461 gccttgctga tgggcgccgg cgccctgtcc atcgcggcct gttcttcggg caaggccagc
     2521 gcggcggcgg acccgttcgc gtcctcgccg atccgccgca cgactgacgc ccaatggcgc
     2581 acgcggctgc ccggccttag ctatgacgtc ctgcgtcatg aggcgaccga gcggccctac
     2641 agcagtccgc tgaacgagga gcaccgcgcc gggaccttcg tctgcaaggc ctgcgacctg
     2701 ccgctgtttc tgtcggcatg gaagttcgaa tccggcaccg gctggcccag cttcttccgc
     2761 gccaacccgg caaatatcgg caccaaacgc gatctggcta tcggtttccc gcgcacggaa
     2821 taccactgcg cacgctgcct cgggcaccag ggtcacctgt tcgatgacgg ccctgcgccg
     2881 accggaaaac gctattgcaa caacggcgtc gccctgcatt tccgtccggg ctgagcaatc
     2941 agcccgggtt gcgagccttc atcgcagact caatcgtcgc gccggtcatc acggcggcga
     3001 tgatgatgaa caggccatag tcgcggccgc tccagaacac cggaacagcc gcgaacagga
     3061 ttacgacaag cggaaatatg ctcgacaggg ccgccgtcgc gggcatgccc acgtgcacca
     3121 tcggcggcgt gactcgcttc atccagcgtt gcatcagcgt gttgccgatg acgtaggcga
     3181 tgacgcagat cgcggccatc agcggatatt tgatctcgcc gcccggctgg ccgaacacca
     3241 ccgccgtcag gatcagcaac gccagagcga cgccggtgct gaccgccagc aggaagtttg
     3301 gttcgagacg cttcaagact tcaccttcac ataggaaccg ggggccggct cgatggcctt
     3361 gagcggaccc ttagccggat cgcgcgccgg aacccagtcg cctgaccgct catgcagcca
     3421 ctcagcccag tgggtccacc aactgccggg atgctcctcg gcgttcgcct gccattcggc
     3481 cagggtcgcg ggcagggcgt cgttgaccca gtgttgatac ttgttggccg agggcgggtt
     3541 gatgaccccc gcgatatggc ccgaccccgc cagggtcagc gtcacgtccg cgttcctgaa
     3601 cgcctgcgcg gaacgataga ccgaattcat cggcgcgatg tggtcctcgc ggctggcctg
     3661 gaaatagagc gggatggtca ccttcgacag gtcgaccgtc tcgccgccaa tgttgaactg
     3721 gcccttcgag agcaggttct ggccgtacat cgagcggagg taatccatgt gcaacgcctt
     3781 gggcatccgc gtctggtcgg cgttccagaa cagcagatcg aacgccgcag gcgacttgcc
     3841 catcagatag ttggacacga agaacgacca gatcaggtcg ttggctcgaa gcgcgttgaa
     3901 ggtctccgcc atcgccgcgc cgggtagcac accgcccgcc gcgtccatct gttcctcgac
     3961 ctgcgccagc caatgctcgt cggtaaagag cagcaggtcg cccgcctcct cgaagtcgtg
     4021 ctgggccgcg aagaaggtgt tggcgccgac ccgcgtgtcg tcattcgccg ccatatgagc
     4081 cagcgccgcg cccatcaggg tgccgccgat gcaatagccg accgtgttca gcgtctccga
     4141 ctgcgactgc tccagcgcct tctcgatggc gaggtagatg cccttttcga gatagtcgtc
     4201 gaagccgaac gccgccttgt ccacgtccgg attgacccac gaacagacga acacggtaaa
     4261 gccctgcgct gataaccagc ggatcagcga gttctccggc tgcaggtcca tgatgtagaa
     4321 tttgttgatc cacggcggga agatcagcag cggaatggcg cgctgtttat cggtcgcggc
     4381 gtcgaactgg atcagttcga acaactcgtc gcgccacacg acctggcccg gcgcggtggc
     4441 cacgttctcg ccgacgacga acttgccgta gtcggcctgg ctgatcgcca gcttgcccgc
     4501 gccgcgctcg aggtcggctg cgaaattctg catgcccttg accagcgact cgccgttgct
     4561 atcggccatg gccttcaacg ccgccggatt ggaggcgagg aagttcgacg gcgaaaaggc
     4621 atcggtcagc agcttggtga agaactcggc gcggcgcttg gtcaggggat cgacgtcctc
     4681 gaccgaagcg atcaggccgt tcatccagtc cgatgtgacc agatatgact ggcgcatcat
     4741 gtcgaacatc gggttctcgg accatgcggc gtccttgaac cgcttgtctt tcgatggcgg
     4801 ctgggtggtt tcgcccgagg cgcgccgggt cgtggaagac cacaggtcca tataacggct
     4861 gaacaggtcg gcctgggcgc tgaacagctt gtcgggccga gcggccagac tgctcatcac
     4921 cgaggtcatg gccgggccga cgttgaatgg atcgggtgac agggccgctt gcccctcttt
     4981 ccgaggattg gccgcctggc tcattgcagc ctcggcgatg gccgactgag cggtcatggc
     5041 agccttggcg atattcatcg acagcgtctc aagcatctgc atctgctcag cgccgggcat
     5101 ggcgaacgaa cccgcgctga acgtcggagg gacctcaggc tcaggagcgg cttccggcgg
     5161 ggcaggggcc tcgcccggct tcgtcgcctc cggcggtgct gccagcgctg gcgcttccga
     5221 ttccatcttc acctttgcgc gaggacgcgg cggtttcact gacgttggcc gcccggactt
     5281 gcgcggtgcg gcgacagcga cgggctccgg cgtcgacgcg accgcttcga caggcggctc
     5341 tgacggcatt ggagtggcgg aggccttggt cggtcgcttg gccatcggag gggtccttcg
     5401 agcgtttccg gcgtggggga ctacagccgc ccttccgcct gatgcgatca tggcatatga
     5461 ccacgtctga aatgaacgct cgcgtgacga aaaagatgat gcggccgatg tttacggtcg
     5521 tcacggttgt cgggctgacc tgttcgctcg gcgcctgcgc tggtgggttc aatccagaaa
     5581 ccgacgccag ctcccccgtc gcgccccgcg tgcaggcgct ggtggacgcc aaccgcgtct
     5641 atccgcagtg gaaggatttc ccgcgctcgt cagatcccgt gccgcaaccg gccgagatcg
     5701 cccagcaggt gaacaccctg cgcgcgaccg gcggcgcgct ggctggcgag gtctcggcac
     5761 tgcagtggca gaatgctgat tcggcggcgt ttgagcgcga agtcgccgcc cgtgtcgctg
     5821 cgactccgat cgctccggct accgctcaga ccgccgccga gatcgaggcc tacgcccaaa
     5881 ccctgcgcaa tcgcgccaag gcgccgcctc cgattagccg tcagtagact ccacagacac
     5941 aagttcgtct cgcccggtcg atagaatccg gggatcatag ctcctcaagg agacctcaat
     6001 ggccgacatg gcccctttcg atacccagcg gctggaccag agccgcccgg gtgcgcgtcc
     6061 ggttccgcag accgggttct ccttctccag cctcgccgcc gacgccgtca gcgtgacgga
     6121 ggccgccgcc atcgcctcat tcgcccagat cgggcgcggc gatgagcagc aggcggacca
     6181 gctcgcggtc gatgcgatgc gcaccggcct caaccgcctc gagatcgacg gtctgatcgt
     6241 catcggcgag ggcgagcgcg acgaagcgcc catgctctat atcggcgaag cggtcggcac
     6301 cggccggggc cccgccgtcg atatcgcgct cgatccgcta gaaggcacga ccctgacggc
     6361 caaggcgatg cccggtgcgc tctgtgtcct gtcgatgtcg gcgggcggcg gcatgctgca
     6421 tgcgcccgat acctatatgg acaagattgc catcggcccc ggctatgcgc ccggcacggt
     6481 cgatctggac atgagcccgg ccgagaacgt ccgctcgctg gcctcggcca aaggcgtcga
     6541 tccgcgcgac atcactgtat gcgtcatgga ccgcccgcgc cactcggccc tgatcgccgc
     6601 cctgcgcgag gtcggcgcca aggtcatgct gatcggcgac ggcgacgtcg ctggcgtgat
     6661 tcacacggcg gaccccgaca ccggcatcga tctttatctc ggctcgggcg gagcgcctga
     6721 aggcgtgctt gcggcctcgg cgctcaaatg cgtcggcggc catttccagg gacggctggt
     6781 gttccgcaac gaccacgagc gcggacgcgc tcaccgcatc ggcatcaccg acctgaaccg
     6841 caaatacgac ctgcatgagc tggtctcgac cgacgccgtc ttcgccgcga ccggcgtcac
     6901 cagcggcgcc ctgcttgacg gggtcaagat cgagcgtggc atgatcagca cccacaccgt
     6961 cgtgatggac tcatctacct gcacggtgcg ccgcatccgc acgacgcggc ctctctagtg
     7021 gctgatggtg gcattcccgt cggcgggata gctcagttcc tggacgtctc gcgctcgctc
     7081 tcgggccgag cctggcgtca gcgtccggcc gagccgtcgg taacccgcgc tcatatgcag
     7141 gcgctgggct tgcaggagcc cctcgcacga gccctcgcgt cgcgcggcgt gcgagcggat
     7201 cagggcgcag acttcctgac ccccacgctc aaggccctgt tccccgatcc ctccagcttc
     7261 atggacatgg acgcggcctc gactgccatt ctcgacgcga tgacagccgg atccagcatc
     7321 catatcttcg ccgactacga cgtggacggc gcctccagcg cggcgcttct ggtgcgctgg
     7381 ttccgggcca tggggtcgga actgccgatc tacgtccccg accgcctgac cgagggctat
     7441 ggccccagcg ccaaggcgtt cgacaccctg aaggcgctcg gcgcagatct ggtcatcacc
     7501 gtcgactgcg gcgcggcggc caatgaggcg gtggcccacg cgggcgatat tggcctgaat
     7561 gtcgtggtca tcgaccacca catgatgcgc gaggagccgc cgaaatgtct ggcggtcgtc
     7621 aacccgaacc ggcccggctg taactcggga cagggcaatc tggcggcggc gggcgtggtg
     7681 ttcgtgctgc tggccgctct gaatcgcgaa gcgcgggcga gaggattgtt tgcggaccgc
     7741 gagcagcccg acatccgcca gtggctcgac ctcgccgcca tgggcgcgat ctgcgacgtc
     7801 accggcctga ccggcttcaa tcgcgccctg acgggccttg gcctgcgcgt catgtcagac
     7861 tggaagaacc ccgggctcaa agctctgttg tccgccgcga aagctgaaga aggcacggcc
     7921 aagtcgaatc acgcaggttt catcctcggc ccccgcatca atgcgggtgg tcgcatcggc
     7981 aagtcggacc tcggtgcgcg actgctgtcc accgacgatc ccgaagaagc ccgcgccatc
     8041 gccgaggaac tggatgccct gaacattggc cgccgcgaga tcgagcggct ggtcaccgaa
     8101 gaggccaccc gccgcgctga ggccacccgc gcccacgatg atggctccgc gatggtggtc
     8161 atcgcaggag acgactggca tccgggcgtg gtcggaa
//
LOCUS       DASVTM010000402         9157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_732289,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000402 DASVTM010000000
VERSION     DASVTM010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732289"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1237)
                     /locus_tag="VF633_11035"
     CDS             complement(125..1237)
                     /locus_tag="VF633_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX8471639.1"
                     /translation="MSLLFSPHTVGPLTLKNRIIIAPMCQYSAVDGVVQPWHAQHLGK
                     LAISGASLVIVEATGVEADGRISWADAGLWNDEQEAAFATLLKGIRTYSDTPIGIQLA
                     HAGRKASTNPTWIERGGPASGDKAWETSSASAVAFKDGWHTPVALDEAGMQRVVDAFV
                     QSAQRADRAGFDLVELHAAHGYLLTQFLSPISNLRTDEFGGSLENRMRFPLRVAAALR
                     DAWPRTKALGARLNGSDWVEGGITPDEAVAFSQALHDLGYDYLHLTSGGNVAVAKIPG
                     DQPGYQVPFAQTVKAAIPDATVVAVGMILDAEQAEQIIASGQADVVAIARAVLDDPNW
                     GHHAASALGEPEGLPNQYERATDSTWPGFKRVHSPA"
     gene            1525..1923
                     /locus_tag="VF633_11040"
     CDS             1525..1923
                     /locus_tag="VF633_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471640.1"
                     /translation="MRALKGLALAAAIMPLLGACVIYDSDAEDTVSVRVGSHTTTAVS
                     EPLETLRAVRFEPGALVVVAGSNGCTDASSFAVEVSESDGPTGITLTRQRPDLCRALV
                     PEGVELRWTYDELGLKPGTAVKVANPVLLP"
     gene            2014..3057
                     /locus_tag="VF633_11045"
     CDS             2014..3057
                     /locus_tag="VF633_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX8471641.1"
                     /translation="MSSTPPPNAQLPDAQLPDAGRNWVDAHAPERLKPWLKLGRFDRP
                     IGIWLLLLPGWQGIALAHARYSEAPGWYAVWLFVGFGIGACLMRAAGCAFNDIVDRDI
                     DARVARTALRPIPSGRISVRQAWAFVVGSSLISLLILLTLNLPAILLGVGSLALVAAY
                     PFMKRITWWPQAWLGLTFNWGALMGFAAAITVAAAGFIERMGGLNPLPGVLIAEPVWG
                     FGEVLPVLFLYAGGVFWTLGYDTIYALQDIEDDAMVGVKSSARRLGAAAARGVAIFYG
                     LAVLSAFSAGWAAGLGPLFYVVVCVYALHLTRQVRTLRLDNATLALKLFKSNREAGFI
                     LLGAIALGAVHFV"
     gene            3068..3916
                     /locus_tag="VF633_11050"
     CDS             3068..3916
                     /locus_tag="VF633_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HEX8471642.1"
                     /translation="MTLSRQTTLKALLVAAAVAATLTGCNRDKEKAATPAAAPPTVAA
                     TPAVAGAPFEFKSETPFAKVELTLPQEIKSQPELHAQLYAAGVKDLREFMEGAQADRT
                     EAGGDEGTPAYDSEIGFDTPVETGKLFSIARSDYEFTGGAHGNTSASGVLWDKALKRA
                     IAPTSLLRPGVNMAALDGLLCAALNVEKKKRDPEAETLRLAGKADDMWNCPHAADTPV
                     VLVPGTQPGKAGGLLFLIGAYEAGPYAEGPYEVPVPQTAIRSLLAPAYADEFAGTPVL
                     SEEPAN"
     gene            complement(3920..4864)
                     /locus_tag="VF633_11055"
     CDS             complement(3920..4864)
                     /locus_tag="VF633_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8471643.1"
                     /translation="MFNPFTGCASARPLPKVAFILAIVAAISVSSHHARAEPALQPQA
                     ASNAFTSDRLSVEVIGSGPDVILIPGLASSREVWLPLATRLAATHRVHLVQLAGFAGE
                     PWSHGDGAFVAPEVAELARYIQAARLGDPAVIGHSLGGFSGLVLAQQHPELVGRLMIV
                     DSLPFYSALFGPQVTAEQARPFADQAVAAILAADDATFASQQTQTAAGLVRDPATRVA
                     ILDWSMRSDRHAIAAALKDVMTTDARPGLAAMTTPVTALYASDADGGAPAAGADAIWT
                     REYATLPGVKLIRVDAARHFIMADQPARFDALVDDFLK"
     gene            complement(4906..5103)
                     /locus_tag="VF633_11060"
     CDS             complement(4906..5103)
                     /locus_tag="VF633_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8471644.1"
                     /translation="MKNSLKLLRVQRGWTQEQLGQELGVSRQAVNALETEKHDPSLDL
                     AYRIAVLFAQPVEEIFENPHR"
     gene            complement(5100..5504)
                     /locus_tag="VF633_11065"
     CDS             complement(5100..5504)
                     /locus_tag="VF633_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471645.1"
                     /translation="MMLAWKNGTPAHRRFIIRTMAFMIPYALVNVGAIFGAFDEIIGR
                     PVAWGLALAIAAPVVGQIWATLSLIRDSDEFVRALTAKQFIVAAGLAMAVAVLWGFGE
                     SYAGAPHLPAWLVYALFWFFWGVTAPFIRTSR"
     gene            complement(5591..5971)
                     /locus_tag="VF633_11070"
     CDS             complement(5591..5971)
                     /locus_tag="VF633_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471646.1"
                     /translation="MRSLRLIVSAVALAGGILIGQGVTPLASSAQAASFACARARSAD
                     ERAICGNRVLEDRDVELSVLYGLITKLVPMGSRDSIRREQTQWLSQRGRCGADVPCLR
                     RSYDRRIATLRATIDSRVVSEGPF"
     gene            complement(6151..7011)
                     /locus_tag="VF633_11075"
     CDS             complement(6151..7011)
                     /locus_tag="VF633_11075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8471647.1"
                     /translation="MIFDGCALHDVRSISKSVVSLLWGIADGEGKTPPLDTKVVSLYP
                     ELAMLARDGREDITIRHLLTMSTGLDWDEENYGALSNPETGLYWRTSQVRQTFARRVV
                     ASAGSRFNYCGGNAAILADLLARYTGHPLPQYAEEHLLKPLGIQNWSWVNDYRGRPLA
                     FSGLRLAPRDLLKIGQLLLSGGQFDGRTVVPAAWIQASTTRHIATGDGLHYGYQWWLG
                     DVASSGGRHPYIAGFGNGGQRLYAVPVLDLVVAITAGNYGKPEQRLSNDLFRRIAATA
                     IPAGSTLHLS"
     gene            complement(7422..8186)
                     /gene="map"
                     /locus_tag="VF633_11080"
     CDS             complement(7422..8186)
                     /gene="map"
                     /locus_tag="VF633_11080"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX8471648.1"
                     /translation="MIKSPAEVEIMAEAGRLLASVFTHIDAQTLMGMSTLQIDALVEA
                     YIVDTLHARPASKGQYGYAFTLNSSCNEVVCHGVPSADEILQDGDIVNLDITLEKNGF
                     IADSSKTYLIGDVATPAKRLVRATYDAMWKGIRAVRPGATLGDIGHAIEQHAKRNGYS
                     VVRDYCGHGIGREMHEKPQVLHFGKPGTGLKLREGMVFTIEPMLNQGVRTVRTEDDGW
                     TVVTADRKLSAQFEHTVAVTKTGVRVLTLRPEERAA"
     gene            complement(8291..8386)
                     /locus_tag="VF633_11085"
     CDS             complement(8291..8386)
                     /locus_tag="VF633_11085"
                     /inference="COORDINATES: protein motif:HMM:NF023331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471649.1"
                     /translation="MGIVNIEDELHEQLRRASKAACRSTNGQAAF"
     gene            8522..>9157
                     /locus_tag="VF633_11090"
     CDS             8522..>9157
                     /locus_tag="VF633_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8471650.1"
                     /translation="MTRITDPEAFILANTRLQIVPHTPEISLWLADEITPIWRLTEEE
                     LGEMGLPPPFWAFAWAGGQGLARWILDNPNEVAGKRVVDFATGSGLVGVAVMKAGAAE
                     VLCADIDPFCGAAVKLNAAANGMALNFTDVDLLDAPPPQVDVICAGDVCYEKPMTDRV
                     LAWLAKARANGTRVLIGDPHRTYFPKEGLEFLAEYRVPTTRELEDFEIKRSG"
BASE COUNT         1581 a         2798 c         3184 g         1594 t
ORIGIN      
        1 cacgtcgccc gaggacgagg gcgtcgaggt caagtggtcg ttcgaggaac tcggcctcaa
       61 gcctggggcg cgggtgtcgg tcgacaaccc ctaccagcta ccccagaccc tataaccaac
      121 cgggtcaggc gggggagtgc acccgcttga agcccggcca cgtgctgtcg gtagcccgct
      181 catattggtt cggcaggcct tccggctcgc ccagagcgct ggcggcgtgg tggccccagt
      241 tgggatcgtc gagcacggcg cgggcgatgg cgacgacgtc ggcctgaccc gaggcgatga
      301 tctgctcggc ctgttcagcg tccaggatca tgccgacggc gacgactgtg gcgtcaggaa
      361 tggcggcctt cacagtttgg gcgaacggga cctgataacc gggctggtcg ccggggatct
      421 tcgccaccgc gacgttgccg ccgctggtca ggtgcagata gtcgtagccg aggtcgtgca
      481 gcgcctggct gaaggccacg gcttcgtccg gcgtgatgcc gccctccacc cagtccgagc
      541 cattcaggcg agcgcccagc gccttggtgc gaggccaggc gtcgcgcagg gcggccgcga
      601 cccgcagcgg gaagcgcata cggttctcca gcgagccgcc aaactcgtcg gtgcgcagat
      661 tggagatcgg cgacaggaat tgggtcagca gatagccgtg ggcggcatga agctcgacca
      721 gatcgaagcc tgcccgatcg gcgcgttgag cggattggac gaaggcgtcg acgacgcgct
      781 gcatgccggc ttcgtcaagc gcgaccggcg tgtgccagcc atccttgaaa gctacagcgg
      841 aggccgacga cgtctcccac gccttgtcgc cgctggcagg gccgccgcgc tcgatccacg
      901 tcggattggt cgaggccttg cgaccggcgt gcgccagctg gatgccgatg ggggtgtcag
      961 aataggtgcg gatgcccttg agcagggtcg cgaacgcggc ttcctgttcg tcattccaca
     1021 gacccgcgtc agcccacgag atccggccgt cggcctcgac gcccgtggcc tcgacgatca
     1081 ccagcgacgc gcccgagatg gccagcttgc ccagatgctg ggcgtgccag ggctgaacca
     1141 cgccatcgac ggcggaatac tggcacatcg gcgcgatgat gatccggttc ttcagcgtca
     1201 gggggccgac ggtgtgggga gagaagagca ggctcatgca aggcgtccgg agtgtgggga
     1261 ggctcggtag ctatgccggt tgcggatcgg ttcaaggggt gaggcgtgat ggaagccgtg
     1321 ggtttaagcc tttccgatta ttcacgtgtg ttttgaagcc tgtcgggtga tcacgtctga
     1381 caagcttcag tacaggatac cgccaatgct tcgtctcgca ctcgcaatcg ccatcgccgc
     1441 cccgctgctt tcgggctgca tcatcatcgc caccgacaag ccgacgacgc gcgtggttca
     1501 gaccgcgcct gccgaaggcc agtaatgcgc gcgctcaagg gtctggcgct ggcggcggcg
     1561 atcatgcctc tgctgggcgc ctgcgtgatc tatgacagcg acgcagagga caccgtctct
     1621 gtccgcgtcg gatcgcacac cacgaccgcc gtcagcgaac ccttggaaac cctgcgggcc
     1681 gtgcggttcg agccgggtgc cctggtcgtt gtggccgggt cgaacggatg caccgacgcc
     1741 tcgagcttcg cggtcgaagt gtccgagagc gacggtccta cgggcatcac cctgacacgc
     1801 cagaggccgg acctgtgcag ggcgctggtt cccgaaggcg tcgagctccg ctggacctat
     1861 gacgaactcg gcctgaagcc cggtacggcg gtcaaggtcg ccaatccggt cctgcttccc
     1921 taggcccacg gagggctgag accatagaag ggcgagcgtg gctgcgttcg cccctcttct
     1981 cgtgaacctc cctgaagggc tagaacggcg gccatgtctt ccactccccc gcccaacgcc
     2041 caacttcctg atgcccagct tccggacgcc gggcgtaact gggtcgatgc ccatgcgccg
     2101 gagcggctga agccctggct gaagctgggg cggttcgatc ggccgatcgg tatctggctg
     2161 ctgctgctgc ctggctggca agggattgcg ttggctcatg cccgctatag cgaggcgccc
     2221 ggctggtatg ccgtgtggct gttcgtcggc ttcggcattg gcgcctgttt gatgcgggcg
     2281 gcgggttgcg ccttcaatga catcgtcgac cgcgacatcg acgcccgggt ggcgaggacg
     2341 gcgctgcggc ctatcccgtc ggggcgaata tcggtcaggc aggcgtgggc gttcgtcgtc
     2401 gggtccagcc tgatcagcct gttgatcctg ttgacgctga acctgccggc catcctgctg
     2461 ggcgtcgggt cgctggcgct ggtggcggct taccccttca tgaagcggat cacctggtgg
     2521 ccgcaggcgt ggttggggct gacgttcaac tggggtgcgc tgatgggatt cgctgcggcg
     2581 atcacggtcg cggccgccgg atttatcgag cggatggggg ggctcaatcc gctgcctggc
     2641 gtgctgatcg ccgagcccgt ctggggcttc ggcgaggtgc tgccggtgct gttcctctac
     2701 gcgggcggcg tgttctggac gctgggctac gacacgatct acgccctgca ggacatcgag
     2761 gacgacgcca tggtcggggt aaaatcctcg gcgcgacggc tcggcgcggc ggcggcgcgg
     2821 ggtgtggcga tcttctacgg cctggcggtc ctgagtgcgt tcagcgcagg ctgggcggcg
     2881 gggctgggac cgttattcta tgtcgtggtt tgcgtctatg cgctgcatct gaccaggcaa
     2941 gtacggacat tgcgcctcga caacgccacg cttgcgctca aactgttcaa gtcaaaccgg
     3001 gaagccgggt tcatcctgct gggcgccatc gcgcttggcg ccgtccattt cgtctaagga
     3061 cctccgcatg acgctctccc ggcaaacgac cctgaaagcg ctgctggtgg cggctgcggt
     3121 ggcggcgacc ctgacgggct gcaatcgcga caaggagaag gctgcgacgc ccgcggccgc
     3181 gccgccgacg gtcgcagcga cgcctgcggt cgcgggagcg ccgttcgagt tcaaatccga
     3241 aactcccttc gccaaggtcg aattgaccct gccgcaggag atcaaaagcc agcccgaact
     3301 gcatgcgcaa ctctatgccg caggggtcaa ggatctccgc gagtttatgg aaggcgcaca
     3361 ggccgatcgc accgaggctg gcggcgatga gggcacgccc gcctacgact cggagatcgg
     3421 gttcgacacc ccggtcgaga cgggcaagct cttcagcatc gcgcgctcgg actatgagtt
     3481 cacgggcggg gcgcacggca acaccagcgc gtcgggggtg ctttgggaca aggccctgaa
     3541 acgggccatc gcgccgacca gcctgctgcg ccccggggtc aacatggcgg cgctcgacgg
     3601 tctcctgtgc gcggcgttga acgtcgagaa gaagaagcgc gacccggagg ccgagacgct
     3661 gaggctggcg ggcaaggccg acgacatgtg gaactgcccg cacgcggctg acaccccggt
     3721 cgttctggtc cccggcacgc aacccggcaa ggcgggcggg cttctgttcc tgatcggggc
     3781 ctatgaagcc ggcccttatg ccgaggggcc atacgaggtg cctgtgccgc agaccgcgat
     3841 ccgcagcctg ctggctcccg cttatgccga tgagttcgcc ggaacgccgg tactgtccga
     3901 ggaaccggcg aactaggtcc tacttcagga agtcgtcgac cagagcgtcg aagcgggcgg
     3961 gctggtcggc catgatgaag tggcgagcgg cgtccacccg gatcagtttg acgcccggca
     4021 aggtcgcgta ttcgcgggtc cagatggcgt ctgctccggc ggcgggcgcg cccccgtcag
     4081 catcagaagc atagagcgcc gtgaccggcg tcgtcatggc ggcgaggccg ggccgggcgt
     4141 cggtggtcat gacgtccttg agggctgcgg ctatagcgtg gcggtcgctc ctcatcgacc
     4201 agtccagaat ggcgacgcgg gtggcgggat cgcggacgag accggcggcg gtctgggtct
     4261 gctggctggc gaaggtcgcg tcgtcggcgg cgaggatggc ggcgacggcc tgatcggcga
     4321 aggggcgggc ctgctcggcc gtgacctgag gcccgaacag cgcgctgtag aagggcaggg
     4381 agtcgacgat catcagacgc ccgaccaact caggatgctg ctgcgccaga accagacccg
     4441 agaagccgcc gagcgaatgg ccgatgacgg cgggatcgcc gagacgggcc gcctggatgt
     4501 aacgggccag ttcggcgact tcgggggcaa cgaaagcgcc gtcaccgtgg ctccagggct
     4561 cgcctgcgaa accggcgagc tggaccagat ggacgcggtg cgtggctgcg aggcgggtcg
     4621 ccagcggaag ccagacttca cgcgaggaag cgagaccggg gatcagaatg acgtcagggc
     4681 cggagccgat gacctcgacc gacaagcgat cggaggtgaa ggcgttggag gcggcctggg
     4741 gctgcagagc aggctcagcc cgagcgtgat ggctgctgac gctgatggcc gcaacgatgg
     4801 cgaggatgaa tgcgactttc ggcagtggac gtgctgaggc acatcccgtg aagggattga
     4861 acatcgtttt tccttttaag gttaggctga cttgtccttg cggggtcagc ggtgggggtt
     4921 ctcgaagatt tcctcgacag gctgggcgaa cagcacggcg atccggtagg cgaggtcgag
     4981 ggacgggtcg tgcttctctg tctcgagggc attgacggcc tgacgcgaga cgccgagctc
     5041 ttgaccgagc tgctcctggg tccagccgcg ttgaacgcgc agtagtttaa gactgttttt
     5101 catcgcgacg tccggatgaa gggagccgtg acgccccaga agaaccagaa cagggcgtag
     5161 accaaccagg caggcaggtg gggggcgcca gcatagcttt caccgaaacc ccagaggacg
     5221 gcaaccgcca tagcgagacc ggcagcgact atgaactgtt tggcggtaag agcgcgcacg
     5281 aactcgtcgg agtcgcggat caggctcaag gtggcccaga tctggccgac gaccggcgcc
     5341 gcgatcgcta gcgccaatcc ccaggcgact ggcctgccga tgatctcgtc aaaggcgccg
     5401 aaaatagccc ccacgttcac caacgcatag gggatcatga aagccatggt gcggatgata
     5461 aagcgtcggt gcgctggtgt tccatttttc caggcaagca tcatgatgtg tcctccaact
     5521 caatgtcagg agaacatgac atgtgcgata tgtcgcgtca acctgacatt tagtcaggtc
     5581 gccctgacgc ctagaatggg ccttccgaaa cgacgcggct atcgatcgtg gcgcgcaggg
     5641 tggcgatgcg gcggtcatag ctgcggcgga ggcaggggac gtcggcgccg cagcggccgc
     5701 gctggctgag ccactgggtc tgttcgcggc ggatgctgtc acgcgaaccc atgggcacca
     5761 gcttggtaat cagcccgtag agcaccgaca gttcgacgtc gcgatcctct agcacccggt
     5821 tgccgcagat ggcgcgttcg tctgccgacc gggcgcgggc gcaggcgaaa ctggcggcct
     5881 gcgcggacga ggccaggggc gtgacgccct gaccgatcaa aatgcccccc gcgagggcga
     5941 cggccgaaac tatgaggcga agcgagcgca tgtcgggtct ccgatgacgg gatcgacgca
     6001 cgctagtccc gcgatcgtgg cggcgctatc ctcatgccaa gactgtcgcc cccggaggtg
     6061 ccgatgctca tccggctgtg cgctgggatc ggtgctgcgg gaagcgagcc gaggaggtcg
     6121 tgcacagcgc cctgctgaac atgagcctgg ctagctgagg tgaagcgttg atccggcagg
     6181 gattgcagtc gcagctatcc tccggaacag atcattgctg aggcgctgtt cgggcttgcc
     6241 gtagttgcct gctgtgatcg ccacgaccag atccagcact ggcacggcgt aaagacgctg
     6301 ccccccgttt ccgaagcccg cgatgtaggg gtggcgcccg cctgacgagg cgacgtcacc
     6361 aagccaccat tgatagccgt agtgaagacc atctcccgtc gcgatgtgcc gcgtcgtgga
     6421 ggcctggatc caggctgcag ggaccaccgt ccttccgtcg aactggccac cagaaagcag
     6481 gagctggccg atctttagaa ggtcgcgggg cgcgaggcgt aagccggaga aggccagggg
     6541 ccgacctcga tagtcgttca cccagctcca gttctggatg ccgagcggct tcaacaaatg
     6601 ctcctcagca tattgcggca gtgggtgtcc ggtatagcgc gcaagcagat cagccaggat
     6661 cgcggcattg ccgccgcaat agttgaaccg cgaacctgcc gacgccacca cgcgacgggc
     6721 gaaggtctgc cggacctggg atgtgcgcca atacaggccg gtctccgggt tcgacagagc
     6781 gccgtagttt tcctcgtccc agtccagtcc ggtcgacatc gtcagaagat gtcggatcgt
     6841 gatgtcctcc cggccgtcgc gggcgagcat ggccagctca ggatagaggg acacaacctt
     6901 cgtatcgagc ggcggcgtct taccctctcc atccgcaatg ccccacaaaa gcgagacgac
     6961 ggatttggag atcgaacgga cgtcatggag cgcgcacccg tcgaaaatca ttttccggga
     7021 ccctcgtccg acagattcat cgtggcccgt ccgataccgc tcggcgatct ttgcgccgga
     7081 gcgccaaatc gaaaggccgt ggaagttcgt ctgagtgctt tcgaagtctg cgagacaggc
     7141 cctgagcgct gtgccgtcga aactgatgtt caggaggtcc ggttgggtcc cgcatccgaa
     7201 gcggctcacc gaaggccgag gctcggtcac ggaagggctg cacgctcctg cagcaagggc
     7261 gacggaggct tgcaccatcc accttcgcgt tcgattggcg gtcatggtgc gaaagtccca
     7321 gcggaacgcg cacccgaaca gcaaagcttt gtggggtcaa tggcagaggc ccggactgtg
     7381 cgggatggac gcgcgctggt gtgcgccctg accgtgctgc gtcaggccgc ccgttcctct
     7441 ggccgtagcg tcagcacccg cacgccggtc ttcgtcaccg caaccgtatg ctcaaactgg
     7501 gcggacagct tgcggtcggc ggtgacgacg gtccagccat cgtcttccgt gcggacggtg
     7561 cggacgccct gattgagcat gggctcgatg gtgaagacca tgccttcgcg cagcttgagc
     7621 ccggtcccgg gcttgccgaa atggaggacc tgaggctttt cgtgcatctc gcgaccgatg
     7681 ccgtgaccgc aatagtcgcg gacgaccgaa tagccgttcc gtttggcgtg ttgctcgatc
     7741 gcgtggccga tgtcgccgag cgtggctccg gggcggacgg cgcggatgcc cttccacatg
     7801 gcgtcgtagg tggcgcggac gagacgcttc gccggggtcg ccacgtcgcc gatcagatag
     7861 gtcttgctgg agtcggcgat gaagccgttc ttctcgagcg tgatgtcgag attgacgatg
     7921 tcaccgtcct gcaggatttc atccgccgac ggcacgccat ggcagacgac ttcattgcat
     7981 gagctgttga gcgtgaaggc atagccgtac tggcccttgc tggcaggccg tgcgtggagc
     8041 gtgtcgacga tgtaggcctc gaccagcgcg tcgatctgca gggtgctcat gcccatgagt
     8101 gtctgggcat cgatatgcgt gaacacggag gcgagcaggc gaccggcctc ggccatgatc
     8161 tcgacctcgg cggggctctt gatcatgccg ccagatcgac gggggcgacg ccggcggcct
     8221 tcaattcacg cgccacgagt tcctgaaacg tcaggcccgg attgagctcg caaagcatgc
     8281 cgatcttgat ttagaacgcc gcctggccat tggtcgatcg gcacgcggcc tttgaggcgc
     8341 ggcgcaactg ttcgtgcagt tcgtcctcga tattgacgat gcccatcgaa ggctcctgac
     8401 tatatgattc gtatgtgttt catatattcc gagatcgagt ggggcaaggg acttccgttt
     8461 catcgggaaa gccgcgatag gatggggcgg aactgtctcc cgccctacgc acggccgcgc
     8521 cttgacccgc atcactgatc ccgaggcctt catactggcc aacacgcggc tgcagatcgt
     8581 gccgcatacg ccggagatct cgttgtggct ggcggacgag atcacgccga tctggcggct
     8641 gaccgaagaa gaactgggcg agatgggctt gccgccgccg ttctgggcgt tcgcctgggc
     8701 gggcgggcag gggctcgcgc gctggatcct cgacaatccg aatgaggtcg ccggcaagcg
     8761 ggtcgtggat ttcgccacgg gctcgggatt ggtcggggtt gcagtgatga aggcgggggc
     8821 ggctgaggtg ctgtgcgccg atatcgatcc gttttgcggt gcggcggtaa agctgaacgc
     8881 ggcggccaac ggcatggccc tgaacttcac cgatgtggac ttgctggacg cgccgccccc
     8941 tcaagtcgat gtgatctgcg ccggggacgt ctgctacgaa aaacccatga ccgaccgggt
     9001 gctggcctgg ctggcgaaag cacgagccaa cgggacgcga gtgttgatcg gcgatccgca
     9061 ccggacctac ttccccaaag aaggtctcga gtttctggcc gagtaccggg tgccgacgac
     9121 gcgggagctc gaggacttcg agatcaagcg ctcaggc
//
LOCUS       DASVTM010000403         6303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_887998,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000403 DASVTM010000000
VERSION     DASVTM010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887998"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VF633_11095"
     CDS             complement(<1..296)
                     /locus_tag="VF633_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HEX8471651.1"
                     /translation="MSAEDQKRRAGEAAALRVEAGMIVGLGTGSTAAWFVKALAARGL
                     KGLRCVPTSEKTADLARELGLVLSTLDDTPHIDLTVDGADEIGPGLALIKGGGAA"
     gene            complement(495..1838)
                     /gene="glmU"
                     /locus_tag="VF633_11100"
     CDS             complement(495..1838)
                     /gene="glmU"
                     /locus_tag="VF633_11100"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX8471652.1"
                     /translation="MTTRPRAAIILAAGQGTRMKSPLPKVLHPIGHRAMLDHAIDAAE
                     ALGCERIVVVVGSHSPEVRAHVVARLGEDAIAVQDPPLGTGHAVRAAEAVLGDFVGQV
                     VVTYGDVPLLKAADIEPVFAAHDGVTVIGFEARDPGAYGRLVMDGDTLAAITEAKEAS
                     AEVLAITACNSGVMAAPVGLLFSLLAEVTNENAKGEYYLTDVVELARKRGAPTRAVFA
                     GEDQVMGVNAQGELAQAEALFQSVQREAFLAAGVTMSAPETVHFAWDTQIGAGTTIEP
                     FVIFGPGAKIAERVRIRGFSHIEGATVASGAEVGPYARLRPGADLGEDVKVGNFVEVK
                     NVAMARGAKANHLSYLGDGTVGAGANIGAGVIFCNYDGFNKAKTVVGAAAFVGSNSAL
                     VAPVTIGAGAIVGSGSVIVEDVPADALALARGRQVNKAGAAAVFRDKAKAKKAKT"
     gene            1908..2591
                     /locus_tag="VF633_11105"
                     /pseudo
     CDS             1908..2591
                     /locus_tag="VF633_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            complement(2629..3222)
                     /locus_tag="VF633_11110"
     CDS             complement(2629..3222)
                     /locus_tag="VF633_11110"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8471653.1"
                     /translation="MARRREEMVEETRASLIRAARHAFATKGYAAAAMDELTANAGLT
                     RGALYHNFGDKKGLLQAVIDQIDAEMLARTRAASEHAETPWAAFVQEGIAYIEMSLEP
                     EIQRIMLLDGPAVLGDPSRWPNQTACLRTTTDAVQALIDAGTVKPVDAEAVARLVNGT
                     ALNAALWIAAADDPEAVMAKSVEAFRQLVSGLLRDDG"
     gene            3321..3719
                     /locus_tag="VF633_11115"
     CDS             3321..3719
                     /locus_tag="VF633_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8471654.1"
                     /translation="MTQREAIFPAGRQALYEINRYSAAIRSGDLLFVSGQVGSRDDGS
                     AEPDFQQQVELAFANLVAVLKAAGCTVDDIVDVTTFHTDPEAQFATIMAVKDKVFGGP
                     PYPNWTAVGVNWLAGFDFEIKVIARIPEAA"
     gene            complement(3765..4355)
                     /locus_tag="VF633_11120"
     CDS             complement(3765..4355)
                     /locus_tag="VF633_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471655.1"
                     /translation="MSKSVRPVIVGLIVTFVTAALLGFIGSMGGADMGFKPALVGMIF
                     GAFTYYIMANLAGNVKGKAATPEAKAAAVALRPEPGKAMLIVFREGFIGKAAGMNVIV
                     DGKPRAQLKSPRFTAIPLEPGSHDLETAFGALAGKQSRASHETFSVVAGDVVIFQAKI
                     SMGALKNTINVERMTPNQTIANRFADMVMIAPLDAA"
     gene            complement(4469..4696)
                     /locus_tag="VF633_11125"
     CDS             complement(4469..4696)
                     /locus_tag="VF633_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEX8471656.1"
                     /translation="MTDAHNPKLDETPASNTEKDPDEWVSGGDPMTGAQASYLKTLSE
                     EAHKPEMFDDKIDKAEASRRIDTLKSGLDLQ"
     gene            complement(4782..5696)
                     /locus_tag="VF633_11130"
     CDS             complement(4782..5696)
                     /locus_tag="VF633_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX8471657.1"
                     /translation="MAPPKGDAALTDMRFSLSDALGKARQPAIFKRYGMVSNWLAESV
                     LHSRLTVPVASGRPMMIATLSQPGARPPIQRAQYLSACIVGVVTLVTMAVSIWVEFVA
                     LSAIVTSDDAAVTAASIAGGSQAIRQAAVLLFVCFISDLAVGVAAYVVLSPISHGLAM
                     LGVLFRVANAAVLSASALALIVALRLVSDAPYLEAFTLEQQQALARLVMGVRADTMSF
                     GWVFLGLGQATFAWLWFQSRYIPRALAVLGIGASTALAVVPLIVLVRPEIGNVIGIAF
                     MAPMFFYEVLLGLLLVTRGIRPGKADIR"
     gene            5836..6261
                     /locus_tag="VF633_11135"
     CDS             5836..6261
                     /locus_tag="VF633_11135"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX8471658.1"
                     /translation="MQNILAFSGRSSRRSYGLFTLAFAAIVLGLLAGQSDTPFTQLLS
                     TPWLVLGRAVDDLIQIPSASIDFVVSIAVTGAFLWMFAAMTVCRLRDIGQSPWWTILV
                     MLSGIVVLAMIALSLVPPADRAAAKEAAAGLSARAATRA"
BASE COUNT         1106 a         2169 c         1938 g         1090 t
ORIGIN      
        1 gcggcgccgc cgcccttgat cagcgccagc ccgggaccga tctcgtcggc gccgtccacg
       61 gtcaggtcga tgtgcggcgt gtcgtccagc gtcgacagca ccagccccag ctcgcgcgcc
      121 agatcggcgg tcttctccga cgtcggcacg cagcgcagcc ccttcagccc ccgcgccgcc
      181 agcgccttga cgaaccacgc cgccgtcgac ccggtgccaa ggcccacgat catccccgcc
      241 tcgacccgga gagcggcggc ctcgcctgcg cggcgttttt ggtcttcagc agacatcgac
      301 gcctcgcaca aaagagccct tcccctcgag ggggaagggt tgggatgggg tggaggcacg
      361 gacggaaccg ggaggcacga agacaccgat gcagaaaaac aaacatgacg gtgtgtcagc
      421 gtgtcccttc aacagggcga ctccacccca tccccggccc ttccccctcc aggggaaggg
      481 agcagacacg gtaatcatgt cttggccttc ttcgccttgg ccttgtccct gaacaccgcc
      541 gccgccccag ccttattcac ctgccgcccc cgcgccagcg ccagcgcatc ggccggcacg
      601 tcctcgacga tcaccgagcc ggacccgacg atagcccccg caccgatcgt caccggcgcc
      661 accagcgccg agttggaccc cacgaaggcc gccgcgccca ccaccgtctt cgccttgttg
      721 aagccgtcgt agttacagaa gatcacgccc gcgccgatgt tcgcccccgc gcccacggtc
      781 ccgtcgccga gataggacag gtggttggcc ttggcgcccc tggccatggc gacgttcttg
      841 acctcgacga aattgccgac cttcacgtcc tcgcccagat cggcgcccgg ccgcagccgg
      901 gcataggggc cgacctcggc ccccgatgcc accgtcgcgc cctcgatatg gctgaagccc
      961 cggatgcgga cgcgttcggc gatctttgcg ccggggccga agatgacgaa cggctcgatg
     1021 gtcgtgccgg cgccgatctg ggtgtcccag gcgaaatgca cggtttcggg cgcgctcatg
     1081 gtcacgcccg ccgccagaaa cgcctcgcgc tgcacgctct ggaacagggc ctcggcctgc
     1141 gccagttcgc cttgggcgtt gacgcccatg acctgatcct cgcccgcgaa caccgcccgc
     1201 gtcggcgcgc cgcgctttct cgccagctcg accacgtccg tcagatagta ttcgcccttg
     1261 gcgttctcgt tggtgacctc ggccagcagc gagaacaaca gccccaccgg cgcggccatg
     1321 acgcccgagt tgcaggcggt gatggccagc acctccgccg aggcctcctt cgcctcggtg
     1381 atggcggcca gcgtgtcgcc gtccatgacc agccgcccgt aggcgccggg gtcgcgcgcc
     1441 tcgaacccga tcacggtgac accgtcgtgg gccgcgaaca cgggctcgat atcggcggcc
     1501 ttcagcagcg gcacgtcgcc gtatgtcacc accacctgac cgacgaaatc gcccagcacg
     1561 gcctcggcgg ctcgcaccgc gtggccggtc cccagcggcg ggtcctgaac cgcgatggca
     1621 tcctcgccca gccgcgcgac gacgtgggcg cggacctcag gcgaatggct ccccaccacc
     1681 accacgatcc gctcgcaccc cagcgcctcg gcggcgtcga tggcgtgatc cagcatggcg
     1741 cgatgcccga tcgggtgcag caccttgggc agcggcgact tcatccgcgt cccctgaccg
     1801 gcggcgagga tgattgcagc tctgggacgg gtggtcatga atcggtccgg cggctagtga
     1861 gcacgcttgc ggtctagccc atcggcgcgg cccatgggag ccgaactatg aaccccgatc
     1921 gcgatctcga aggctggacc atcgccttcg acctggacgg cactctggtc gagacccacg
     1981 aggacctcgt cggcacgctg aaccggatgc tgttgaagca cggcctgccc gaggctccga
     2041 tggacaccgc ccgcgacctg atcggcggcg gcgcccgggc cctgctgcag catggcttca
     2101 tcgcggccgg cgcgtcatgg gaggacgccg atcaggaggc gctgttcgac gccttcatcg
     2161 ctgactacat cgagcatatc gccgaccact cgcaaccgtt cgacggcgtt gtcgagacgc
     2221 tggaagctct gtcggaacgc ggcgccaagc tggtcgtggc caccaacaaa cgcaccgacc
     2281 tgtcggaact gctgctggcc aagctggacc tgacccggca tttcgccgcc atcgtcggcc
     2341 cggacaaggt cagcgcgcgc aaaccctcgg gcgcccacct gagcgaggcc gtgctgctcg
     2401 gcggcggtga tcccaccatg gccatcatgg tcggcgacgc ctcgcccgac gccgacgccg
     2461 cccgcgacgc cggcatgccc tgcatcgttg tcaccttcgg ctacacccag acgccgcccg
     2521 aagacctcgg cggcgacgtc ctcatcgacg cgttcgagga cgtcgaggaa gccatcgacg
     2581 gcctcctcac cgacctacgt cgccaaggcg ctggcgggct tatagcctct agccatcgtc
     2641 ccggagcagt ccggacacca gctgtcggaa ggcctcgact gacttggcca tcaccgcttc
     2701 tggatcgtct gcggcggcga tccacagggc ggcgttcagc gcggttccgt tgaccaagcg
     2761 ggccacggcc tcggcgtcca ccggcttgac ggtgcccgcg tcgatcagcg cctgcacagc
     2821 atcggtcgta gtgcgtagac aggccgtctg gttcggccag cgtgacggat cgcccagcac
     2881 ggccgggccg tccagcagca tgatccgctg aatctctggc tccagcgaca tctcgatata
     2941 ggcgatgcct tcctgcacga acgccgccca cggcgtctcg gcgtgctcgg atgcggctcg
     3001 cgtccgcgcc agcatctcgg cgtcgatctg gtcgatgacg gcctgcagca ggcccttctt
     3061 gtcgccgaaa ttatgataga gcgcgccgcg cgtcagcccg gcgttcgccg tcaactcgtc
     3121 catcgccgcc gccgcatagc ccttggtcgc aaaggcatgc cgcgccgccc ggatcaggct
     3181 ggccctcgtc tcctcgacca tctcttcgcg tcgcctggcc atgccgccct ctattctaca
     3241 tacgctccgt atgccagttg acatacgtcg tgtatgcggt gatctataag catacgatcc
     3301 gtatatcaat ggagacgacg atgacccaac gcgaagccat ctttcctgcc ggtcggcagg
     3361 ccctttacga aatcaaccgc tactcggcgg ccatccggtc gggcgatctg ctgttcgtct
     3421 ccggccaggt cggcagccgg gacgatggct cggccgagcc agacttccag cagcaggtgg
     3481 aactggcgtt cgccaatctc gtggcggtcc tcaaggcggc cggttgcacg gtcgatgaca
     3541 tcgtcgacgt gaccaccttc cataccgacc ctgaggcgca gttcgccacg atcatggccg
     3601 tcaaggacaa ggtcttcggc gggccgccct atcccaactg gacagcggtg ggcgtaaact
     3661 ggctcgcggg cttcgatttc gagatcaagg tcatcgcccg cattccggaa gcggcctgat
     3721 tcaatcgact ggcggcgggc gggatcgcga cccgccgccc cgtcttacgc ggcgtcgagt
     3781 ggcgcgatca tcaccatgtc ggcgaatcgg ttggctatgg tctggttggg cgtcatccgc
     3841 tcgacgttga tggtgttctt caaggcgccc atgctgatct tggcctgaaa gatcaccacg
     3901 tctcctgcaa cgacggaaaa cgtctcgtgg ctggcccggc tctgcttgcc ggccagcgcc
     3961 ccgaacgccg tttccaggtc gtgagatccg ggctcaagcg gaatggcggt gaaacggggg
     4021 ctcttcaact gcgcgcgcgg tttgccgtct acgatgacat tcatgcccgc cgccttgccg
     4081 atgaaaccct cgcggaacac gatcagcatc gccttgcccg gctccgggcg gagagcgacc
     4141 gccgcggcct tggcctccgg cgtggcggcc ttgcccttca cgttcccggc cagattagcc
     4201 atgatatagt aggtgaaggc cccgaagatc atgccgacca gcgcaggctt aaaccccata
     4261 tcggcgccgc ccatcgaccc gatgaaaccg agaagggccg ctgtgacgaa ggtcacgata
     4321 aggccgacga tgacgggtct cactgatttg ctcacggacg atcctgcgac tggttaaagc
     4381 tgagcagtac aacgattccg cagcttgact gttccgaccg gccatccgca gtgtgaaatg
     4441 cgcgcccaaa gcacgcgcat cattggacct actgcaggtc gagcccggat ttcagcgtgt
     4501 cgatccggcg cgacgcctcc gccttgtcga tcttgtcgtc aaacatctcg ggcttgtgcg
     4561 cctcttcgga cagcgtcttc agataggacg cctgagcccc cgtcatcgga tcgccacccg
     4621 agacccactc gtcagggtcc ttctcggtgt tcgaagcggg cgtttcatca agtttgggat
     4681 tgtgtgcatc ggtcatgttg ggaaaaagcg agagttccgc gtttgttcca atcaatgacc
     4741 ccatttccat catccttggc gagcggcttt gtcgcgcaaa cctagcggat atcagctttg
     4801 ccggggcgga tgcctcgggt cactagaagc agccccagca ggacttcgta gaagaacatc
     4861 ggcgccatga aagcaatccc gatgacgttt ccgatttccg gtcgaaccag aacgatcagc
     4921 ggaacgacgg ccaaggccgt ggatgcgccg atcccaagca cggccagggc tcgcggtatg
     4981 tatcttgact ggaaccagag ccaggcgaac gtggcttgac ccaggccgag gaagacccag
     5041 ccgaagctca tggtgtcagc gcggaccccc atgaccaacc gagccagagc ctgctgctgt
     5101 tcaagcgtga aagcctccag atagggggcg tcggaaacga gtcgaagcgc cacgattagc
     5161 gcgagggcgc ttgcggaaag gacggccgcg ttggccacgc ggaaaagaac gcccagcata
     5221 gccaaaccat gactgatagg gctgagcacg acataggccg caacgcccac cgccagatcg
     5281 ctgatgaaac agacgaatag caggaccgcc gcctgacgta tggcctggga cccgcctgcg
     5341 atgctggcgg ccgtaacggc ggcatcatcg gatgtcacaa tcgcgctcaa cgccacgaac
     5401 tcaacccaga tcgagaccgc catcgtgacg agcgtgacga cgccgacgat gcaagcagag
     5461 agatactgag cccgttggat cggcgggcgt gcgcctggct ggctaagggt ggcgatcatc
     5521 atgggtctcc cggaggccac ggggaccgtc aagcgggagt gaagcacgga ctccgcgagc
     5581 cagttactta ccataccgta acgcttgaag atcgcgggtt gccgggcctt gccgagcgcg
     5641 tcgctcaggc tgaaccgcat gtcggtcaag gcggcatcgc ccttgggcgg cgccaaccag
     5701 cagtctgtcg gcgtcgctgg cgaaagtctg gttctagacc agacgcgcat gtctgtaatc
     5761 gacccatagc ggacgtttca gaggttgcca ggccggatgg aatgcttacc gtagcgtgcg
     5821 cagggggggc gcaatatgca gaacattctc gcattctcgg ggcggtcaag caggcgttcg
     5881 tacggcctct ttacgctcgc tttcgctgct atcgttttgg gattgctggc aggccaatcc
     5941 gacacgccct tcactcagct gctgtcaacg ccttggctgg tcctgggacg cgcagtcgat
     6001 gacctgatcc agatcccctc ggccagcata gactttgtcg tctccattgc agttaccggc
     6061 gcatttctct ggatgttcgc agcgatgacc gtttgcagac tgcgcgacat aggacaatcc
     6121 ccgtggtgga cgatcctagt gatgctttcc gggatcgtcg tactcgcgat gattgccctg
     6181 tctctcgttc ctccggcaga ccgtgcagcg gcgaaagagg cagcggcggg tttgtcggca
     6241 agagcagcta ctcgagcata gcgtccgctt tccaccccaa agggcatccg gcacgtctgc
     6301 ttt
//
LOCUS       DASVTM010000404         4880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_39957, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000404 DASVTM010000000
VERSION     DASVTM010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39957"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VF633_11140"
     CDS             complement(<1..463)
                     /locus_tag="VF633_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8471659.1"
                     /translation="MTAMAAIVICAVLFAGGRGLAAGSVGDVLRVRFGGDGERTRVVI
                     DLGASAQGRVVEEGTAGQVVLTLTGVAPRADITGDGSGLVNTYAVNPVGTASRVELRL
                     SRAAQIERRFLLPPGDGVASYRYVIDLKATGPARAVANGARPVASRSAAPRR"
     gene            985..3753
                     /locus_tag="VF633_11145"
     CDS             985..3753
                     /locus_tag="VF633_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX8471660.1"
                     /translation="MSKTMLIDAAHAEETRVAIVDGRQVEEFDFESKTKRQLRGNIYL
                     AKVTRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQIPAADREAIMAEANSADDEEDE
                     HARDSDDGESEGGMAEEERLKRRLMRRYKIQDVIRRRQILLVQVVKDERGGKGAALTT
                     WLSLAGRYCVLMPNTGKGGGISRKITQSSDRRRLKAAASALDVPRGMGLIIRTAGAKR
                     TKAEIKRDYEYLLRLWETIRETTLASNAPSLIYEEENLVRRAVRDMFDKDFDGIQVEG
                     IEGFKEARDFMRVLMPSQAKKVHLYQGSRPLFAANGIEEVLTGIHQPVVTLKSGGYLV
                     INQTEALVAIDVNSGRATKERNVEQTAFKTNCEAAVEAARQLRLRDLAGLVVIDFIDM
                     DESKNNRTVERILKDALSEDRARIQMGKISGFGLMEISRQRRRLGVLEGATELCPHCA
                     GAGRVRSAESAALMVLRAVDIEAGKNGAGSINLRVSTAVALYILNHKRDYLQHLLATR
                     GLAVIIQIDDSLAQGESAIDRTETNEDFVAAEPTLALSDLLNDGFDDNAYDDEDEDDE
                     DDVIDDEDEVALDREDTDDDEPRERQEVEGRGNRGRKRRRGGRRDEETAVAAPVEGAP
                     LAASEDDDDDDSEAGRRRRRGRRGGRRVREDGDTANTGSFFWVRGRTPSLDDPYVWFD
                     PINPEPRAPREQRVVSEGVAPDVEERVGERPEGELAVDREGGDREGGRSRRRRRGRGR
                     GTDRGAEGGLTHEVKVETRATSEGMPADGSPHTAMAVMSPVDDAEEAAPVIAAEPQRR
                     RVRRKPAGAVTDAVAEIAPEALGAFEDEPAPQADVVEPNEAEPIGAAVELDVTPVEAI
                     AERAEVEALIAQPEPEAIPVDELPPATVVEHVPAAKPEADLSSIIAADPAQIAAPPEK
                     PKRGWWRR"
     gene            3910..>4880
                     /locus_tag="VF633_11150"
     CDS             3910..>4880
                     /locus_tag="VF633_11150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8471661.1"
                     /translation="MSSLARVETILKASSHGAGRKGLSRLLAAATAAVFAFGVAGPAS
                     AQSAIRDTEIEGIIRGWSDPVFTAMGLNPEEVEVLLINDPSLNAFALKGRIMGINTGL
                     IEQTKTPNELLGVMAHEAGHIKNRHLLRGGVQNAGMQPFLMTMALGALAAVAGQPGAG
                     AMLMANSQYFGTLGALRYMQSQEGEADISGARALEAAGESGKGLVDFFENFRSQEVFS
                     DARRYPYFRSHPLSGQRIDALRRPVEAASHYSHADSAERQADHDLIVAKIHAFMDAPS
                     QTLRTYLETDTSFPARYARAIAWYRDGQTQRALDATEALIAEDVDNP"
BASE COUNT          919 a         1672 c         1545 g          744 t
ORIGIN      
        1 cgcggcgagg cgcggccgat cggctagcga ccggacgggc gccattagcg actgcgcgtg
       61 caggccctgt ggccttcagg tcgatgacat agcgatagct agccactcca tcgccgggcg
      121 gaagcagaaa gcggcgctcg atctgggcgg cacgcgacag ccgcaactcg acgcgagacg
      181 cggtgccgac gggatttacg gcgtaggtgt tcacgaggcc ggagccgtcg ccggtgatat
      241 cagcgcgcgg cgctacccct gtcagggtca ggacgacctg tccggccgtg ccttcctcga
      301 ccacacggcc ttgagcagat gcgccgagat cgatgaccac tcgggtccgt tcgccatcgc
      361 cgccgaagcg gacgcgcagc acgtcaccga ccgagccggc agcaagaccc cgtccgccag
      421 cgaacagcac ggcgcagatg acgatagcgg ccatcgccgt catcgcccac tcgcgcgtgc
      481 cccactggga aaatctggaa atccaccgac cgtgcattct gacggtaacc caccttcttt
      541 gttggatgca ggatggcaga gacgcgtttg gaatgggtta aactaagagg ctttttggcc
      601 ccctcagacc caagcctttt cattcctttc aggtttgcct atgctttagc tgctcgcgga
      661 gtgttcgcgc ctgccgatcc gttcggtccg gcgcgtcgac ctcgccggcg atgactttca
      721 acctatccga ggatctccgg cttctgagcc gctgattcga agaccgggcc cactcgcagc
      781 ctcacttctg gcgcgtccgg gctcaggacc gactgaaccc catgggccta tccgcctgcg
      841 ctcccatccg gccattgatg gccgtcgtcg aattcatcga cgggaagacg ctcgccctgg
      901 cctgcaatcc tattcggcga gccgccccca ccccgaaggc cacggccacg ggttccggca
      961 gagcgcgcca gagagaacca ttccatgtca aagactatgc ttatcgatgc ggcgcacgcg
     1021 gaagaaacgc gcgtcgccat cgtggacgga cgccaggttg aagaattcga cttcgagtcg
     1081 aaaaccaaac gccagcttcg cggcaatatc tatctcgcca aggtcacccg cgtagagccc
     1141 agcctgcagg cggcgttcgt cgaatacggc ggcaaccgcc acggcttcct ggccttcaac
     1201 gaaatccacc cagactacta ccagatcccg gccgccgacc gcgaagccat catggccgag
     1261 gcgaactcgg cggatgacga ggaagacgaa cacgctcgcg acagcgacga cggcgagtcc
     1321 gaaggcggga tggccgagga agaacgcctg aagcgtcgcc tgatgcgccg ctacaagatc
     1381 caggacgtca tccgccgtcg tcagatcctg ctggtgcagg tcgtcaagga cgagcgtggc
     1441 ggcaagggcg cggccctgac cacttggcta tcgctggccg gtcgctactg cgtcctgatg
     1501 cccaacaccg gcaagggtgg cggtatctcg cgcaagatca cccagtcctc ggaccgccgt
     1561 cgcctgaagg ccgccgcctc ggcgctcgac gtgccgcgcg gcatgggcct gatcatccgc
     1621 acggccggcg cgaagcgcac caaggccgag atcaagcgcg actatgaata cctgctgcgt
     1681 ctgtgggaga cgatccgcga gaccacgctg gcgtcgaacg cgccctcgct gatctatgag
     1741 gaagagaacc tcgtccgccg cgccgtgcgc gacatgttcg acaaggattt cgacggcatc
     1801 caggtcgaag gaatcgaggg cttcaaggaa gcgcgcgact tcatgcgggt gctgatgccc
     1861 tcgcaagcca agaaggttca cctgtatcag ggctccaggc ccctgttcgc agccaacggc
     1921 atcgaggagg tcctgacggg catccaccag ccggtcgtga cgctgaaatc cggcggctat
     1981 ctggtgatca accagaccga ggcgctggtc gccatcgacg tcaactcggg ccgcgccacc
     2041 aaggaacgca acgtcgaaca gacggcgttc aagaccaact gcgaagccgc cgtcgaggct
     2101 gcccgccagc tgcgcctgcg cgacctcgcc ggtcttgtgg tcatcgactt catcgacatg
     2161 gatgagtcga agaacaaccg cacggtcgaa cgcatcctca aggacgccct gtccgaagac
     2221 cgcgcccgca tccagatggg caagatctcc ggcttcggcc tgatggagat cagccggcag
     2281 cgtcgccgcc tcggcgtgct ggaaggcgcc accgagctct gcccgcattg cgccggcgcc
     2341 ggccgcgttc gctccgccga gtccgccgcc ctgatggtgc tgcgcgcagt tgacatcgag
     2401 gccggaaaga acggcgcggg gtcgatcaac ctgcgcgtct cgaccgccgt cgccctctac
     2461 atcctgaacc acaagcgcga ctatctgcag catctgctgg cgacgcgggg tctggcggtc
     2521 atcatccaga tcgacgatag cctggcccag ggcgaaagcg ccatcgaccg caccgagacc
     2581 aacgaggact tcgtcgccgc cgagccgacc ctcgccctgt ccgacctgct gaacgacggc
     2641 tttgacgaca acgcctatga cgacgaggac gaagacgacg aagacgacgt catcgatgac
     2701 gaagacgaag tcgccctcga ccgcgaggac accgacgacg acgagccgcg cgagcgtcag
     2761 gaagtcgaag gccggggcaa ccggggtcgc aaacgccgtc gtggcggtcg tcgcgatgag
     2821 gaaaccgctg tcgccgctcc ggtcgaaggc gcgcctctcg ccgcttccga agatgatgat
     2881 gacgacgaca gcgaagccgg ccgtcgccgc cgtcgtggac gtcgtggcgg tcgtcgggtc
     2941 cgtgaggacg gcgacacggc gaacaccgga tccttcttct gggtccgtgg ccgcacgccg
     3001 tcgctggacg atccctatgt ctggttcgac cccatcaacc ccgagccacg cgctccgcgt
     3061 gaacaacggg tcgtctctga aggcgtcgct cccgatgtcg aggaacgcgt tggggaacgt
     3121 cctgaaggcg aattggccgt ggaccgtgaa ggcggtgatc gcgaaggcgg gcgttcgcgc
     3181 cgtcgtcgtc gtggccgtgg tcgcggtacc gaccgtggcg ccgagggcgg cctgacccac
     3241 gaggtcaagg tcgagacgcg cgccaccagt gaaggcatgc ccgccgacgg ttcgccgcat
     3301 acggcgatgg ccgtgatgtc acctgttgac gatgccgaag aggccgcacc ggtgatcgcc
     3361 gctgaacctc agcgtcgtcg cgttcgccgg aaaccggcag gcgcggtgac cgatgccgtg
     3421 gccgagatcg ctcccgaggc ccttggggct ttcgaggacg aacctgctcc tcaagcagac
     3481 gtcgtcgaac ccaacgaggc cgagcctatc ggcgctgcgg tcgagctgga cgtcaccccc
     3541 gtagaggcca ttgcggagcg cgccgaggtc gaggccctga tcgcccagcc agagccagag
     3601 gccatccccg tggatgagct gcccccggca acggtggtcg aacacgtccc ggctgcgaag
     3661 cccgaggccg atctgtcgtc gatcatcgcg gccgacccgg cgcaaatcgc cgcaccgccc
     3721 gagaagccca agcgcggctg gtggcgtcgc tgagccgcaa ggcctggatg acggtaaagc
     3781 ctgattgatc aggatggagc gcggcagccg caaaagggac accgatcccg gcgaccgtcg
     3841 cgctccagtc cattcaactg ggccagtcca gctatcgggg ccgatccaac ggttccgggg
     3901 gagttcatct tgagcagcct tgctcgcgtt gagaccattc tgaaggcttc cagccatggg
     3961 gcgggccgca agggcctgag ccgattgctg gccgccgcaa cagccgccgt gttcgctttc
     4021 ggcgtcgcgg gtccggcctc ggcccagagc gccattcgcg acaccgagat cgaaggcatc
     4081 atccgcggct ggtccgaccc ggtgttcacc gccatgggac tgaaccccga agaggtcgag
     4141 gtcctgctga tcaacgaccc gagcctgaac gcgtttgcgc tcaaggggcg gatcatgggc
     4201 atcaacaccg gcctgatcga acagaccaag acccccaacg aactgctcgg cgtgatggcg
     4261 cacgaggctg ggcacatcaa gaaccgccac ctgctgcgcg gcggcgtcca gaacgcgggc
     4321 atgcagccct tcctgatgac catggcgcta ggcgcgctcg ccgccgtcgc cggtcagccg
     4381 ggcgcggggg cgatgctgat ggccaacagc cagtatttcg gcacgctcgg cgccctccga
     4441 tacatgcaga gccaggaagg cgaggccgac atatccggcg cccgcgcgct ggaagccgcc
     4501 ggtgaatccg gcaagggact ggtcgatttc ttcgaaaatt tccgctcgca ggaagtcttc
     4561 tccgacgccc gccgctaccc ctatttccgc agccacccgc tgtccggcca acggatagac
     4621 gcgctgcgtc gtccagtcga agccgcctct cactacagcc acgccgacag tgccgaacgg
     4681 caagccgacc acgacctgat cgtggccaag atccatgcct tcatggatgc tcccagccag
     4741 accctgcgca cttatctgga gaccgacacc tccttcccgg cccgctacgc ccgcgctatc
     4801 gcctggtatc gcgacggcca gacccagcgt gcgctcgacg ccaccgaggc cctgatcgcc
     4861 gaagacgtcg ataacccata
//
LOCUS       DASVTM010000405         6233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_838072,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000405 DASVTM010000000
VERSION     DASVTM010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838072"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF633_11155"
     CDS             <1..399
                     /locus_tag="VF633_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEX8471662.1"
                     /translation="DLREPRFIGDMPHAWISSDYIRSALDMFVYERDSDHTLVLAAGL
                     PLDWLDTPTGVGVRAVRTAYGALTYGFKREAGVAVLTIAPGVAPPGGFVVQWPEGVDL
                     PARVRIDGRRGEWKGREVVIPAGARRVEFR"
     gene            complement(584..1672)
                     /gene="ugpC"
                     /locus_tag="VF633_11160"
     CDS             complement(584..1672)
                     /gene="ugpC"
                     /locus_tag="VF633_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX8471663.1"
                     /translation="MADVRLSGICKSFGATEVLRGIDLDIKDGEFVVFVGPSGCGKST
                     LLRTIAGLEETSAGDISIGGKRVNDLGPSDRGIAMVFQSYALYPHMSVYENMAFGLKL
                     AKADKAGIDAKVRAAATTLNITDYLDRKPKALSGGQRQRVAIGRAIVREPQVFLFDEP
                     LSNLDAALRVRMRYEFASLHERLKTTMIYVTHDQIEAMTLADRIVVLNAGVIEQVGSP
                     MELYEHPSNLFVAGFIGSPKMNLIPAEIVTASATGSTVRTVGGDALSASVDASRAQPG
                     DVVMLGVRPEHLTLAGSTNALATTASFVETLGHATYAYVTTAGSKDTLTVQLPGDVRP
                     AMGDALNLHVAADQAHLFDADGMAFKRL"
     gene            complement(1931..4057)
                     /locus_tag="VF633_11165"
     CDS             complement(1931..4057)
                     /locus_tag="VF633_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8471664.1"
                     /translation="MPFPASHPSLDRALSDRGYAEPTPVQAAVLEAAPNADGSGRDLL
                     VSAQTGSGKTVAFGLALAPTLLGDEEKFTDFGAPLALVIAPTRELAQQVSTELQWLYA
                     HTGARIVSCVGGMDPKVERRALERGAHIVVGTPGRLRDHLERGALDLSEAKAVVLDEA
                     DEMLDMGFQEDLTFILDAAPAERRTLLFSATLARDIVQLAKTYQRDALRIDTVAGAGS
                     HADIEYKAIRIAPNEIELGVVNVLRYFEAPGALVFANTRERVKHLTASLRERGFSVVG
                     LSGELTQSARSEALQALRDGHARVCVATDVAARGLDLPDLGLVIHAEIPVNKAGLLHR
                     SGRTGRAGKKGVSVLLVSYTRRRKVELMLQSASIVAEWSGPPSAEMILEKDRERLLAD
                     PALTAPVEDEEALALGKLLLERTTPEQVAASLIRLYRQKLPAPEDVYDDDRMKRQQET
                     GKNDRGQPDAPYTDFARGGDMVWFRVNIGRDKNADPKWLLPTICRLGHVTKGDIGSIK
                     IFDRETKFEITKTAEAKFRAAVAASTEDGVTVSDAVAPGPKEKPASRWDKKPSGDGGE
                     RSGDRPERKPWANKTEGGAPRGDKPAWKDREGGAEKKPWVKREDSAPTGEKKPWVKRE
                     DAGSTGEKKPWVKREGSAPTGEKKPWVKKDSSAPKTAWAPASDAHAAPGEKPWGKPKA
                     KPQGGDRPWAPKDAKGKPKGKNKPNG"
     gene            4316..>6233
                     /locus_tag="VF633_11170"
     CDS             4316..>6233
                     /locus_tag="VF633_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8471665.1"
                     /translation="MFKTVLLATTALAVLAGSAIAQQALRPGAPVSGDLRRGDEQLDS
                     GEFVDTWTFQGRAGDRVSLHMSSSDFDPYLLVNGPGDFSEENDDAEEGEATDAGLDLR
                     LPATGTYRVMATSYEADEAGRYSLSMDIGGARGGSLAGQLGSRGPASSQRSAQNNLIE
                     PGDTVRDSLERGDQTLDSGEFADAWRFRGRRGQTYTVSLDSSDFDPYLVVRGPGGLSE
                     DNDDDPSQRGSSNSRVTFTLPADGEVTLSATSYESGESGRYALAVGEGGQVYGDTRNA
                     NGTYAQNLTRQPQRPGNQPASAPSTDAPITVGQTRDGRLGQGARTLPSGEYVNNFTLR
                     GRRGQQLDLRLSSSEFDPYLQLNGPNGFSAFNDDDEDQDDSHDSRLIVTLPADGAYTI
                     AATSYEASESGRYRLSVGDGSTYRPPAPPRPAPGQGPARPGGNNGGVMRIGDTVGGQL
                     QRGDETLDSGEYVDTYSFQGTRGTRVAVELTSSAFDAYTILTTPSGEQIDNDDGEDGT
                     NSRLDTVLSETGEYQVQVTSFAAAETGSYRFSVAPSQGSPRQAAVAGGPRVFAIMVGV
                     SEYGDVADDLPYTDEDAEKLAETLQREGVLNASSVVLTNAEATVGGVRRAFEQVSRQA
                     GPDDMFLFFFSGHGS"
BASE COUNT          987 a         1908 c         2266 g         1072 t
ORIGIN      
        1 gacctgcgcg agccgcgctt catcggcgac atgccgcatg cgtggatcag ctccgactac
       61 atccgatcgg cgctggatat gttcgtctac gagcgcgaca gcgaccacac tctggtgctg
      121 gcggcgggcc tgccgctgga ctggctggat acgccgacgg gtgtcggggt gcgggcggtg
      181 cggacggcgt atggggcgct gacctatggc ttcaagcgcg aggctggcgt ggcggtgctg
      241 acgatcgcac cgggcgtggc gccgccggga ggcttcgtgg tgcagtggcc agaaggcgta
      301 gacctgccgg cgcgggtacg aatcgatggg cggcgtggtg agtggaaggg acgggaggtt
      361 gtgattccgg cgggggcgcg gcgggtagag tttcggtgac ctgacctgac ccctctcccc
      421 ggagggagag ggagggaacc gcgagcgtag cgaagtggga gggtgagggt gaggggggat
      481 ggcgttgaaa gtcggacacg ccacccctca ccctttcccg cgaaggacga tcactacgct
      541 ctcgtgcgct caagccctct cccgctggga gagggtagac cggtcaaagg cgtttgaacg
      601 ccatcccatc cgcgtcgaac agatgggctt gatcggcggc gacgtggaga tttagcgcgt
      661 cacccattgc cgggcggacg tcgccgggga gctggacggt gagggtgtct ttcgagcccg
      721 ccgtggtgac gtaggcatag gtggcgtggc cgagggtttc gacgaaggag gcggtggtgg
      781 cgagcgcgtt ggtgctgccc gccagcgtca aatgttcggg gcggacgccg agcatgacga
      841 cgtcgccggg ctgggcgcga gaggcgtcga ccgaggcgct gagggcgtcg ccgccgaccg
      901 tgcggaccgt ggagccggta gcggatgcgg tgacgatctc ggcggggatg aggttcatct
      961 tggggctgcc gatgaagccg gcgacgaaca ggttcgaggg gtgctcgtag agctccatcg
     1021 gcgagccgac ctgctcgatg acgccggcgt tcaggaccac gatccggtcg gccagggtca
     1081 tggcctcgat ctggtcgtga gtgacgtaga tcatcgtcgt tttcagccgc tcgtgcaggc
     1141 tggcgaactc gtagcgcatg cgcacgcgga gcgccgcgtc gaggttggac aggggctcgt
     1201 cgaacaggaa gacctgcggc tcgcgcacga tggcgcggcc gatggcgacg cgctggcgct
     1261 ggccgccgga cagggctttg ggcttgcggt cgaggtagtc ggtgatgttc agggtggtgg
     1321 cggcggcgcg gaccttggcg tcgatcccgg ccttgtccgc cttggccagc ttcaggccga
     1381 acgccatgtt ttcgtagacc gacatgtgcg gatagagggc gtaggactgg aacaccatcg
     1441 cgatgccccg gtccgacggg ccgaggtcgt tgacccgttt gccgccgatc gagatgtcgc
     1501 ccgcagaggt ctcttccagc ccggcgatag tgcgcagaag ggtggacttg ccgcagccgg
     1561 acgggccgac gaagacgacg aactcgccgt ccttgatgtc gagatcgatg ccgcgcagga
     1621 cctcggtcgc gccgaagctt ttacaaatac cgctgagccg cacgtcggcc atatgttcct
     1681 gcctgcaagt cttgttttgc aggctttgta agcggttaca ggggaaggga gaagcgactt
     1741 ctccgaaggg tctcaccctc tcccggcggg agagggcttg agcgtccggg agctgaaggc
     1801 gatcggcctt gcgcgaaagg gtgaggggct gcggtgtgaa gtcggacacg ccaacccctc
     1861 accctttcgg cttgcgcatc gctaagctct gcgagcctcc agccctctcc cgccgggaga
     1921 gggtgaagac ttaaccgttc ggcttgttct tgcctttggg tttgcccttg gcgtctttgg
     1981 gggcccaggg gcggtcgccg ccttgcggct tggccttggg cttgccccaa ggcttttcac
     2041 ccggcgcggc gtgagcgtcc gaggctggag cccaggcggt cttgggtgcg gacgagtcct
     2101 tcttgaccca cggcttcttc tcaccggtgg gtgctgagcc ttcgcgtttg acccacggct
     2161 tcttctcgcc ggtcgaacca gcgtcttcgc gcttcaccca aggcttcttc tcaccggttg
     2221 gagcgctgtc ttcgcgtttg acccaaggct tcttctcagc cccgccttcg cgatctttcc
     2281 acgcgggctt gtctccacgg ggagcaccac cttcggtctt gttcgcccaa ggcttgcgct
     2341 cgggacgatc acccgaacgc tcgccaccgt cgccggaagg cttcttgtcc cagcggctgg
     2401 cgggcttttc cttggggccg ggcgcgacgg cgtcggagac ggtgacgcca tcctcggtcg
     2461 aggcggcgac ggcggcgcgg aacttggctt cggcggtctt ggtgatctcg aacttggtct
     2521 cgcggtcgaa gatcttgatc gagccgatgt cgcccttggt cacgtgaccc agacggcaga
     2581 tggtcggcag cagccatttc ggatcggcgt tcttgtcgcg gccgatgttg acgcggaacc
     2641 agaccatgtc gccgccacgg gcgaagtcgg tatagggcgc gtccggctgg ccacgatcgt
     2701 tcttgccggt ttcctgctga cgcttcatgc ggtcgtcgtc gtagacgtcc tcaggggcgg
     2761 gcagtttctg gcgatagagg cggatcagcg aggccgcgac ctgttcgggc gtggtgcgtt
     2821 ccagcagcag cttgccgagc gccaaagctt cctcgtcctc gacgggtgcg gtcagggcgg
     2881 ggtcggccag caggcgctcg cggtccttct cgaggatcat ttcggcgcta gggggacccg
     2941 accattcggc cacgatcgag gccgattgca gcatcagctc gaccttgcgg cgacgggtgt
     3001 aggagaccag caggacggag acgcccttct tgcccgcgcg gcccgtacgg cccgaacggt
     3061 gcagtaggcc agccttgttg accgggattt cggcgtggat gaccagaccc agatcgggca
     3121 ggtcgagacc gcgcgcggcg acgtcggtgg cgacgcagac gcgggcgtgg ccgtcacgca
     3181 gggcctgcag cgcctcggag cgggcgctct gggtcagttc gccggacagg ccgacgaccg
     3241 agaagccgcg ttcacgcagc gaagcggtca gatgcttgac gcgctcgcgc gtgttggcga
     3301 acaccagcgc gccgggcgcc tcgaaatagc gcagcacatt gacgacgccg agctcgatct
     3361 cgttcggcgc gatgcggatg gccttgtatt cgatgtcggc gtgcgagccg gcgccggcga
     3421 ccgtgtcgat acgcagggcg tcgcgctgat aggtcttggc aagttgcacg atgtcgcggg
     3481 ccaacgtcgc cgagaacagc agcgtgcggc gttcggccgg ggcggcgtcg aggatgaagg
     3541 tcaggtcttc ctgaaagccc atgtcgagca tttcgtcggc ttcatcgagc acgacggcct
     3601 tggcttccga caggtcgagc gcgccgcgtt cgaggtggtc gcgcagacga ccgggggtgc
     3661 cgacgacgat gtgggcgccg cgttccagcg cacgacgctc gaccttggga tccatgccgc
     3721 cgacgcagga gacgatgcgc gcgccagtgt gggcataaag ccattgcagc tcggtcgaga
     3781 cctgctgggc cagttcgcgg gtcggggcga tgacgagcgc gagaggcgcg ccgaagtcgg
     3841 tgaatttctc ttcgtcaccc agcagggtgg gggcgagggc cagaccgaag gcgacggttt
     3901 taccggaacc ggtctgggcg gagaccagaa ggtcgcgtcc ggagccgtcc gcgttgggcg
     3961 cggcttcgag gacggcggcc tggacggggg tcggctcagc atagccgcga tctgaaagag
     4021 cgcggtcgag cgagggatgg ctggctggga agggcatgaa ttgtctcgta cggcgcttcg
     4081 gtcgaaaacg cgaggtggag cgctttttcg agggcgcgga tgcaggggcc tgaggcgcgc
     4141 gcctgatcct cggggatttg gagcgatcac ttctgaaggg gcgggcgttt gcgcccggaa
     4201 caaggccgaa tggtggtgat atgtcagatt tggaacttag ccggtcataa cccgccgccg
     4261 ttaatgacaa ggcagggcaa tcccgtcact gtggcgacgt gctcggggga gttccatgtt
     4321 caagaccgta ctactggcca cgaccgcact cgcggtgctg gcaggttccg ctatcgcgca
     4381 acaggcgttg aggccgggcg cgcccgtttc cggcgatctg cgtcgaggcg acgagcaact
     4441 cgacagcggc gagttcgtcg acacctggac cttccagggc cgggcgggcg accgggtgtc
     4501 gctgcatatg tcctcgagcg atttcgatcc ctatctgctg gtcaacggcc cgggcgactt
     4561 ctcggaggag aacgacgacg ccgaagaggg cgaagcgacc gacgccgggc tggacctgcg
     4621 cctgcctgcg accgggacct atcgtgtcat ggcgaccagc tatgaagcgg atgaggcggg
     4681 ccggtattcg ctgtcgatgg atatcggagg cgcgcgcggc gggtctctgg ccgggcagtt
     4741 gggatcgcgc ggccccgcgt cctcacagcg atcggctcag aacaacctca tcgagccggg
     4801 cgataccgtg cgcgacagtc tggaacgagg cgaccagacc ctcgacagcg gcgagttcgc
     4861 cgacgcctgg cggttccggg gacgacgcgg ccagacctat accgtcagcc tcgattccag
     4921 cgatttcgat ccatatctgg tcgtgcgtgg tccgggcggg ctcagcgaag acaacgacga
     4981 cgatccctcg cagcgtgggt cgtccaatag ccgcgtcacc ttcaccctgc ccgcagacgg
     5041 cgaagtcacg ctgtcggcca ccagctatga atccggcgag agcggtcgtt atgccttggc
     5101 ggtcggggag ggcgggcagg tctatggcga cacgcgtaac gccaacggca cctatgcgca
     5161 aaacctgacc cgccagccgc agcggcccgg caatcagccc gccagtgcgc cgtccaccga
     5221 tgcgccgatc accgtgggcc agacccgcga cgggcggctg gggcagggcg cgcggacctt
     5281 gccgagcggg gaatacgtca acaacttcac cctgcgtggc cgccggggcc agcagctgga
     5341 cctgcgtctg agctcgtctg agtttgatcc ctatctgcag ctgaacggcc cgaacggctt
     5401 ttcggccttc aacgacgacg acgaggatca ggacgacagc cacgacagcc ggctgatcgt
     5461 gacgctgccg gcggacggcg cctatacgat cgcggcgacc agctatgagg cgagcgagag
     5521 cggccgttac cggctgtcgg tcggtgatgg ctcgacctat cggccacctg cgccgccacg
     5581 gcccgcgccc ggtcagggac cggcccgtcc cggcggcaac aacggcggcg tgatgcgcat
     5641 cggcgatacc gtcggcggtc agctgcaacg cggcgatgag acgctcgaca gcggcgaata
     5701 tgtcgacacc tacagcttcc agggcacgcg cgggacacgg gtggcggtcg agctgacctc
     5761 gtcggcgttc gatgcctata cgatcctgac cacgccgtcg ggcgagcaga tcgacaacga
     5821 cgacggcgag gacggcacca acagccgcct cgacacggtg ctgagcgaga ccggcgaata
     5881 tcaggtgcag gtcacgtcct tcgcggcggc cgagaccggt tcgtaccggt tcagcgtcgc
     5941 tcccagccag ggctcgccgc gtcaggccgc cgtcgcgggc gggccgcgcg tgttcgccat
     6001 catggtcggc gtctcggaat acggcgatgt ggccgatgat cttccgtata cggacgaaga
     6061 cgccgagaag ctggcggaaa cgctccagcg cgagggcgtg ctgaacgcct caagcgtcgt
     6121 gctgaccaac gccgaggcga cggtcggcgg cgtgcgtcgc gccttcgagc aggtgtcgcg
     6181 ccaggcggga ccggacgaca tgttcctgtt cttcttctcc ggtcacggca gcc
//
LOCUS       DASVTM010000406         4219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_94331, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000406 DASVTM010000000
VERSION     DASVTM010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94331"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..466
                     /locus_tag="VF633_11175"
     CDS             98..466
                     /locus_tag="VF633_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471666.1"
                     /translation="MMLSLLMMLASDPVAAASTNDNDLRARMTAVQQTQAQCRNGTVM
                     RADGRVTTVPRLDGEMTMGQAIARTPRVPLRANDLMFRSGDPVQMTLLLERSVDHCSV
                     PISTELSGPGSALPRFGHGH"
     gene            complement(476..1792)
                     /locus_tag="VF633_11180"
     CDS             complement(476..1792)
                     /locus_tag="VF633_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX8471667.1"
                     /translation="MPALKRIAIVGGGFSGAMLAARLAETGLASILINRTPDFGLGVA
                     YSTPFEGHLLNVRSNRMTAVEGRPDDFVDWLQAHHPELGTAHDFAPRKVYGDYLRDRL
                     STVETAHPGLIERRVATVAAITADGLTLSDGQTIQADAVVLCTGNPAPNAASTHGERV
                     ISDPWAPGALERIGAADDVVIVGTGLTMVDMIQSLVARGWTGKATSLSRRGLTPRPHN
                     PTPDTPTPLPPEALSGPLSQRLAAVRALAAKGCWRCVMEAYRPITADLWGQADADLRA
                     RMVRHLRPWWDVHRHRIAARIGWSLDALKAQGRLTIVAGRLGRVETAPDHVTVHWTPR
                     GQGGGGAATAAPLTANWMIDCTGPAHSAAADPLTAPLIASGRARLDPLRLGLDLDAKG
                     RVLHADGAPDPALFVLGPPARAAFWETIAVPDIRKRIEDVVAALAA"
     gene            2083..2796
                     /gene="gpmA"
                     /locus_tag="VF633_11185"
     CDS             2083..2796
                     /gene="gpmA"
                     /locus_tag="VF633_11185"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX8471668.1"
                     /translation="MPRLILLRHGQSQWNIENRFTGWVDVDLTAEGEAQARHGGQLIA
                     EAGFKPTVMFTSVLTRAKRTGALALQAAGLKDVPVIEDWRLNERHYGGLTGLDKAETA
                     KIHGEDQVKVWRRSYDIPPPPLEAGSEWDFNADPRYAGQTIPDTESLKTTLDRVLPYW
                     NEAVAPRLKAGEDVLIAAHGNSLRAIVKHLFGVPDDQIVGVEIPTGNPLEIDLDADLK
                     PTAARYLDQGRAQSLPAIA"
     gene            2793..3437
                     /locus_tag="VF633_11190"
     CDS             2793..3437
                     /locus_tag="VF633_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX8471669.1"
                     /translation="MSTAEADAAQKDMGFMAQAIALATARMGETWPNPSVGCVIVKDG
                     VVLSEAATAPGGRPHAEEQAVPAAGAEVAGATAYVTLEPCGARSSGRQSCAHFLAEAG
                     VARVVVACLDPSPFASGRGTERLRQAGLIVETGLLGDQATVLCEGFLHRLETGRPMVR
                     TSADGEGFDARFVASPRADLVTELKRLGEAGYTRLWTGPGELADALSAQGLLTA"
     gene            3499..>4219
                     /locus_tag="VF633_11195"
     CDS             3499..>4219
                     /locus_tag="VF633_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8471670.1"
                     /translation="MQAATQDPLRRRITQQELNLICTKHDRLWQARPGGARAVFAWMD
                     LSGLDLSWRNLTDADFSAADMSGCDLTGAKLDNATFFGTDLQGSTLIDCSLRRADLRG
                     ACLRSADLSGADLFEADLREGTLATADAKNGFRVVEPKNRDQTQAQGACLAGANLQRS
                     RLSGIIAQAADFSDAIMKDCKLVRANLKQANFRGADLAGADLSGADLTGADLRDAVLV
                     GVTSSMWRTDGAKLEGALTDKG"
BASE COUNT          688 a         1323 c         1608 g          600 t
ORIGIN      
        1 cgagcctcaa gccctctccc gccgggagag ggaggatact ccgggttgtc atgagagact
       61 ccgggtatag gatcggacgt ccaataggag aggcctgatg atgctgtccc tgctgatgat
      121 gctggcgtcc gatccggttg cggcggcgtc gacgaacgat aatgacctca gggcccgaat
      181 gacggccgtc cagcagacgc aggcgcagtg ccgcaacggc acagtgatgc gggcggatgg
      241 cagggtgacg acggtcccgc gtctggatgg cgagatgacg atgggtcagg cgatcgcgcg
      301 tacgccgcgc gtgccgctga gggccaacga cctgatgttc cggtcgggtg atccggtgca
      361 gatgaccctg ttgctggagc ggagcgtcga tcattgctcc gtgccgatct cgaccgagct
      421 gagcgggccg ggaagcgccc tgccccggtt tgggcacggg cattagccga cgcttttagg
      481 ccgctagggc agcgacgacg tcctcgatac gcttgcggat atccgggacg gcgatggtct
      541 cccagaaggc ggcgcgggcc ggggggccga ggacgaacag cgccgggtcg ggggcgccgt
      601 cggcgtgcag gacgcggcct ttggcatcca gatcgagacc gaggcgtaag gggtccagac
      661 gggcgcgacc gctggcgatc agcggtgcgg tcagaggatc ggcagccgcc gaatgggccg
      721 ggccggtgca gtcgatcatc cagtttgcgg tcaggggagc ggcggtggcg gcgcctcccc
      781 ccccttggcc tcggggcgtc cagtggacgg tgacatggtc gggggcggtc tcgacgcggc
      841 cgaggcgtcc ggcgacgatg gtcaggcggc cttgggcctt gagcgcgtcg agcgaccagc
      901 cgatgcgggc ggcgatgcgg tggcggtgga cgtcccacca agggcgcagg tggcggacca
      961 tccgggcgcg caggtcggcg tcggcctggc cccaaagatc ggccgtgatc ggacgatagg
     1021 cctccatgac acagcgccag cagcccttgg ccgcgagcgc gcgcacggcg gcgagacgct
     1081 gggacagggg gccggacagg gcctcgggcg gcaggggcgt cggcgtgtcg ggcgtgggat
     1141 tgtgcggcct cggcgtcagg ccacgacgcg acagggaggt cgccttgccg gtccagcccc
     1201 gcgcgaccag cgactggatc atgtcgacca tggtcaggcc ggtgccgacg atgacgacat
     1261 cgtccgcagc gccgatccgc tccagcgcgc cgggcgccca gggatcgctg atgacgcgct
     1321 cgccgtgggt ggaggcggcg ttgggggcgg ggttgccggt gcacaggacc acagcgtcgg
     1381 cctggatggt ctggccgtcg gacagggtca ggccgtcggc ggtgatggcg gcgacggtgg
     1441 cgacgcggcg ctcgatcaga ccgggatggg cggtctcaac cgtggaaaga cggtcgcgga
     1501 gatagtcgcc gtagaccttg cgcggggcga aatcgtgggc ggtgccgagc tcggggtggt
     1561 gggcctgcag ccagtcgacg aagtcatcgg ggcggccctc gaccgcggtc atcctgttcg
     1621 agcggacgtt gagcagatgg ccctcgaacg gggtcgaata ggcgacgccg aggccgaaat
     1681 cgggggtgcg gttgatcagg atcgaggcga ggccggtctc ggccagacgg gcggccagca
     1741 tggcgccgga gaagccgccg ccgacgatgg cgatccgttt aagggctggc actcaggcgg
     1801 gccagacgag ggcgagggcc gcgagggcga ccgcgatgat ggcggcggcg gcgaagaaca
     1861 ggcgatccga catgggctga gcctatagca gcgcgggcgg cggcgacaac agcttcggga
     1921 agagctggaa cggggcttcg gaatgaccgg cggtcatcaa tccgtcgcgt cccggcttga
     1981 cccccgggag gccgcgtcct aggccaagtg tccgaaatta tccaatcgcg acagtatcca
     2041 aatgcgaccg gcctccccgc cggcgccgcg ccggagccga ccatgcctcg tctgatcctt
     2101 cttcgtcacg gccagagcca gtggaacatc gagaaccgct tcaccggatg ggtcgacgtc
     2161 gacctgacgg ccgagggcga ggcgcaggcg cggcacggcg gccaactgat cgccgaggcc
     2221 gggttcaagc cgaccgtgat gttcacctcg gtgctgacgc gggcgaagcg cacgggcgcg
     2281 ctggcgctgc aggcggcggg gctgaaagac gtgccggtga tcgaggactg gcggctgaac
     2341 gagcgccact atggcggact gaccgggctg gacaaggccg agaccgcgaa gatccacggc
     2401 gaggatcagg tcaaggtctg gcgccgcagc tatgacattc cgcccccgcc tctagaggcc
     2461 ggaagcgaat gggatttcaa cgccgatccc cgctatgccg gacagacgat ccccgacacc
     2521 gagagcctga agaccacgct ggatcgggtg ctgccctact ggaacgaggc cgtcgcgcca
     2581 cggctgaagg ccggcgagga cgtgctgatt gcggcgcacg gcaactcgct gcgggccatc
     2641 gtgaaacacc tgttcggcgt gcctgacgac cagatcgtcg gcgtcgagat cccgacgggc
     2701 aatccgctcg agatcgatct ggatgcagac ctgaagccga cagcggcgcg ctacctggat
     2761 cagggacggg cgcagagcct gccggccatc gcgtgagcac ggctgaggca gacgccgccc
     2821 agaaagatat gggcttcatg gcgcaggcca tcgcgctggc gacagcgcgg atgggcgaga
     2881 cctggccgaa cccgtcggtg ggctgcgtca tcgtcaagga cggcgtggtg ctgtcggagg
     2941 cggccaccgc tccgggcgga cgccctcacg ccgaggaaca ggccgtgccc gccgccgggg
     3001 ccgaggtcgc gggcgcgacc gcctatgtga cgctggagcc gtgcggcgcg cggtcgtcgg
     3061 ggcggcaatc gtgcgcgcat ttcctcgccg aagcgggcgt ggcgcgggtg gtggtggcgt
     3121 gtctggaccc ctccccgttc gcgtcaggtc gagggaccga gcggctgagg caggcggggc
     3181 tgatcgtcga gaccgggctg ttgggcgatc aggcgacggt gctgtgcgaa ggcttcctgc
     3241 accggctgga gacgggtcgg ccgatggtgc ggaccagcgc cgacggcgag ggcttcgacg
     3301 cccggttcgt cgcctcgccg cgcgccgatc tggtgaccga actgaagcgt ttgggcgagg
     3361 ccgggtacac gcgactgtgg accgggccgg gcgagctcgc cgacgcgctg tccgcgcagg
     3421 gactgcttac ggcctaaacg cgtggaaacc ccggttttcg caaggcttcc ttaagcgccg
     3481 ggggagcatc ctgtaatcat gcaggccgcg acacaggacc ctctcaggcg ccggatcaca
     3541 cagcaagagc tgaacctgat ctgcaccaaa cacgaccgcc tgtggcaggc gcgtccgggc
     3601 ggagcgcgcg cagtgttcgc gtggatggat ttgtcgggcc tcgacctcag ctggcgcaac
     3661 ctgaccgacg ccgacttttc cgcagccgac atgtccggct gcgacctgac gggcgccaag
     3721 ctcgacaacg ccactttctt cggaacggac ctgcagggtt cgaccctgat cgactgcagt
     3781 ctgagacgcg ccgacctgcg cggcgcctgt ctgcgcagcg ccgacctttc gggcgccgac
     3841 ctgttcgagg ccgacctgcg cgagggcacc ctggccaccg ccgacgccaa gaacgggttc
     3901 cgggtggtcg agccgaagaa ccgcgaccag acccaggccc agggtgcctg tctggccggc
     3961 gccaatctgc agcggtcgcg cctgtcgggc atcatcgccc aggccgcaga cttttccgac
     4021 gccatcatga aggactgcaa gctggtccgg gccaatctga aacaggctaa tttccgcgga
     4081 gccgacctcg ccggcgctga cttatcaggc gcggacctga ccggggcgga tttgcgcgat
     4141 gcggtgctgg tgggggtcac gtcgtcgatg tggcgcaccg atggggccaa gctcgaaggg
     4201 gcgttgaccg acaagggct
//
LOCUS       DASVTM010000407        12413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_357596,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000407 DASVTM010000000
VERSION     DASVTM010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357596"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VF633_11200"
     CDS             <1..1057
                     /locus_tag="VF633_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEX8471671.1"
                     /translation="EDQSLFLSRYGPAQAAVSDWIDAFSPDEMAAWAEDLGQATFVGS
                     SGRVFPRAMKASPLLRAWLGRLNDLGVVLKTRSRWTGWSDNGLTFEDGTAEEADAVVL
                     AMGGASWPRLGSDGAWVPALEAAGVEVAPLRPSNAGFNVVWSDILIDKAAGQALKPVG
                     LSFGEQTLKGEVMLTRYGVEGGGVYALSAALREAIARDGEAILMLDLRPDLTEAALAE
                     RLGRPRGKDSMTNHLRKAGGLSAAAIAVLRDIPGEMPAGADKLAKRIKGVRLKLTGMQ
                     GIERAISTAGGVTLGAVNQRLMLTARPGVFVAGEMIDWEAPTGGYLLQASFASGVVAA
                     NGVLDWLKAKSSVTESA"
     gene            1054..2199
                     /locus_tag="VF633_11205"
     CDS             1054..2199
                     /locus_tag="VF633_11205"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8471672.1"
                     /translation="MTNLLDISGICPPQFSAVKDAFAANFTDAPEGLNEQAARIAFTV
                     EGEVVVDLWAGQADPRANTPFTDTTLTPVFSTGKAVMALLMATAVEHGDLVYDETVAT
                     LWPAYGAAGKASVTVAQMLSHQDGLPGFDEEVDPAIWFDRQAVLDRLAAQAPMWPPGT
                     ASGYHPITVGYLAGEVFRLTDGRTLGAALRQDFATKFGLDLWIGLPVEEHDRVASIRK
                     PAAGASLGAVDPIKTAAFLAKGSAPGGRGSTEWRTAEIPSANLHGTARDLARILSLVA
                     NGGLLDGETVLSADVLSQLTRERIHGQDKVLPYEISWAAGLMRNEGLNIFGPNPDTVG
                     HCGWGGSCVFADATAKASGAYVMTRQSSHLVGDPRATRLINAFYAAL"
     gene            complement(2246..3175)
                     /gene="rarD"
                     /locus_tag="VF633_11210"
     CDS             complement(2246..3175)
                     /gene="rarD"
                     /locus_tag="VF633_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX8471673.1"
                     /translation="MASPAATETRNALIAGLVCYTAWGVFPLLFMAMARSGFTAGEIL
                     AQRGLWAVLFAAALVMLSGQSAQVRRVLTTPKTVAWLALSTAMILNNWGAYVWATTHH
                     HTIEASLGYYINPLINMVAGMVLFREKIDRWGWIAIGSAAVGVLLQTVALGRAPWLSI
                     LIALTFAAYGIIRKKVAADAQTGLFVECLMMIPVGVVILIWLARTGQMVGFTGAGHWA
                     WSVSSGLATVLPLALFAWSARRLPLSTLGFLQFLAPTLQFAIGVASGEPLTPLRIASF
                     VFIWAGAVVFALAALWRKHAERQAIKTAVDAAC"
     gene            complement(3218..4264)
                     /locus_tag="VF633_11215"
     CDS             complement(3218..4264)
                     /locus_tag="VF633_11215"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX8471674.1"
                     /translation="MGYRVAIVGATGNVGREMLNILEELNFPVDKMHAIASRKSKGMD
                     VAWGDKTIKCEDIETFDFTTVDIVLMSAGGDVSRAWSEKIGKLGPMVIDNSSAFRKDP
                     DVPLIVPEVNPDAIKYAHKKNIIANPNCSTAQLVVALKPLHDAAKIKRVVVSTYQSVS
                     GAGKEGMDELWNQTKAIYGLGDASPKKFPKQIAFNVIPYIGSFNEDGYTDEEQKMSDE
                     THKMLDPNIKLTVTCVRVPVFVGHSEAVTVEFDRPISVEEARAILREAPGVLVVDKHE
                     HDGYITPVDAAGEHAVYVSRIRKDDTVENGLVMWVVSDNLRKGAALNAVQIAQLLDET
                     GTIKSSTGYRSITV"
     gene            4490..5587
                     /gene="zapE"
                     /locus_tag="VF633_11220"
     CDS             4490..5587
                     /gene="zapE"
                     /locus_tag="VF633_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX8471675.1"
                     /translation="MTSRIRSAYDIRIEEGVLAPDPAQAPLIEAMERLETDLAKKGLF
                     GGQPEVRGLYIWGPPGRGKSMLMDLFVSATPEPRKMRAHFHAFMAWVHDLVRQWREGD
                     KKTRQAVFGTHKGDDPIEPIATLIASEARLLCFDELQVTDIADALILGRLFDALFERK
                     VVLAVTSNRAPTELYKNGLNRQLFTPFIAEIEKRCVVIEIDGARDWRLNRLMGSPVWF
                     VGEAGRAPFEALWTDLRGGEPESPAHLKVLGRDVVIERSAGSMARATFDELCAKPLGP
                     QDYLAIAERFHTLFLDAVPALNPDRRHEARRLVTLIDALYEAGPRLVVRADTQPEDLY
                     PAGDGAFEFERTVSRLREMSSASWLERSAHA"
     gene            5655..6188
                     /locus_tag="VF633_11225"
     CDS             5655..6188
                     /locus_tag="VF633_11225"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8471676.1"
                     /translation="MTVLAAALSALMLSMIPATGQTAPVTPASRPAVVAGVSPAAVVA
                     WLNTQGAVAGAVQEEQGRKFVRVDADGLAWRLYFPSCQGDRCSDVQFTTGVANAAITP
                     DLINGWNRDRRFLKAIYEAPTATAPASAVIQYDVLLNSGGPEQLTDHLAIWRSLLPEF
                     ARLTTGGAASAPPAAPK"
     gene            complement(6289..7251)
                     /gene="mdh"
                     /locus_tag="VF633_11230"
     CDS             complement(6289..7251)
                     /gene="mdh"
                     /locus_tag="VF633_11230"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX8471677.1"
                     /translation="MARAKIALIGAGMIGGTLAHVAAREALGDVILFDIAQGTPQGKA
                     LDIAEATAVFGSSVSLKGANDYADIAGADVCIVTAGVPRKEGMSRDDLVSINLKVMKA
                     VGEGIKAHAPNAFVICITNPLDAMVWALQKFSGLPHEKVVGMAGVLDSARFAYFLSEK
                     TGVSVQDINAWTLGGHGDDMVPMVRHSTVGGLPLPDAVAAGFLTQDDLDAIVTRTRKG
                     GGEIVALLKTGSAFYAPAESAIAMAKSYLLDQKRVLPCAVWLSGEYGLSDLYVGVPAL
                     IGAAGVEKIIEFTTNDEEKAMFEKSVASVQGLLQACKDIEPSLA"
     gene            7480..8385
                     /locus_tag="VF633_11235"
     CDS             7480..8385
                     /locus_tag="VF633_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8471678.1"
                     /translation="MRKVTAALIVAAIGGVAAPALAQTPPATIGQQYVPAPWWMRDPV
                     IASIGNVRVEIQANRAGFSANFQSIERSAAEASRKAADQVRALSQSLAAYGVDKVRVE
                     TRLTTRPLYEQYRDEAGTLRDNVRPDKINRYQTEASVQLTIRDVSVLERVYATVVATQ
                     PTSISPVYFNLEPENAWKSNLAAEAMKDARVRALAAASNAGATLGSVRVIDPTGRACQ
                     TDVLAGWPSYGSGGGQATTVNEDVVVTGSRMRSAPMMAAPPAPPAPPPPGQAPSEEAI
                     EAARLALQPPLQTLTETACVVYGLN"
     gene            complement(8370..9179)
                     /locus_tag="VF633_11240"
     CDS             complement(8370..9179)
                     /locus_tag="VF633_11240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8471679.1"
                     /translation="MMTLENLLIIVGLGAIFIVLERLMPLDRAQPILRPGWTTDLLHT
                     FLGGAIIGFCLLLTFTAISVLADHAIPPAVGVAMRAQPGWLQFFEILLLSDLGVYAIH
                     RSAHAVPLLWRFHAVHHSGEHMDWAAAHRVHPVEQVLFSTAAVGPAILLGFSPEPLIA
                     YALVYNVWALLLHANVSLDFGPLRHVIGSPRFHHWHHADQREAYDRNFAALFPFYDHL
                     FGTFRMPKDSLPELYGVDEPVPQDFLGQITHPFRRDRRRATAADASPQFNP"
     gene            9254..9703
                     /locus_tag="VF633_11245"
     CDS             9254..9703
                     /locus_tag="VF633_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX8471680.1"
                     /translation="MPHPSERGLFDTDPRLLENSVAVAGWALCDVRMMDDARFPWLVL
                     IPRVAGASELDDVSAADRPLLMAEMIRAGEIVRRLGEAMHLAVDKVNTAALGNVTSQL
                     HVHVVGRRRDDPLWPDPVWGRHGRASLPQAELTRLADFAARLYSQPR"
     gene            9804..11402
                     /locus_tag="VF633_11250"
     CDS             9804..11402
                     /locus_tag="VF633_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8471681.1"
                     /translation="MGRLVRTACALFSAPVGLCSIIVDDKIAQRTLIGANLASATGRF
                     SPTRVLIAEGPDALMLIEDSLTDPRVMDHEFVTGPAAVRFYAGATVSDRAGKAIGSFS
                     VMDTRPRRPLTGEEVEHLRRFARMAGDLYALERDERASSDKRQTLELAEAMAGVGHFS
                     VDVATAEVTWSDEVYRIHGLEPGSVDPTIHSATGAYHEDDAAVLRGLMEKAMRTGEGY
                     DSLLRLKRADGEERATRSKARCEMDATGKVVRLFGVFQDITEPLQFQRALIEARDAAE
                     AANQAKSDFLANTSHEIRTPLTSIIGFSAFLKSSPTLSDRERHYADRISAASTALLGV
                     INNVLDYSKLEAEAFELDQASFDPRLMAEGAAAMMAEQCREKVLSLDIQISPLVPRTM
                     TADEGRVRQVVLNFLSNAIKFTDVGGIVLKLDLVGGRLKVEVADTGIGISPDGVERLF
                     ERFTQGDSSTTKLYGGTGLGLAISRRLVLMMGGEIGVASTPGDGSTFWFEIPLDQPCG
                     RCSVRCWPRKPSSEIAPFRQTTDG"
     gene            complement(11399..12268)
                     /locus_tag="VF633_11255"
     CDS             complement(11399..12268)
                     /locus_tag="VF633_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8471682.1"
                     /translation="MTSHLIRTVARQHPAYRDDIGDLITRRPIPGPGLDQLDPFLFLN
                     HHGPQTYPPGNQGLPFGPHPHRGFETVTFILEGELAHNDSAGGESIIKAGGIQWMTAG
                     SGLIHAELSPTSFKREGGSMEILQLWVNLPSRLKMTKPDYVGLQAADIPTFITDDGRV
                     TVEPVSGVWGEVTAPIQSLIDIHLAVLRFKPGGMFETPVARGRAVFMYAVAGQVSVGG
                     TAVEPWNLIALNDGEAVSIEAGSDAIILLGHAEPIGEPVASHGPFVMNTREEIIQAIS
                     DYNAGKFGPPPTI"
BASE COUNT         2146 a         4078 c         4051 g         2138 t
ORIGIN      
        1 cgaggaccag tcgctgttcc tgtcgcgtta cggcccggcg caggcggcgg tgtccgactg
       61 gatcgacgcc ttcagccccg acgaaatggc ggcttgggcc gaggatctgg gacaggcgac
      121 gttcgtcggc tccagcggcc gggtgtttcc gagggcgatg aaggcgtcgc cgctgctgcg
      181 cgcctggctg ggtcgcctga acgatctcgg cgtcgtgctg aagacccgat cgcgctggac
      241 cggctggtcc gacaacggcc tgacatttga agacggaacc gccgaagagg ccgacgccgt
      301 cgtgctggcc atgggcggcg caagctggcc ccggctgggt tccgacggcg cctgggtccc
      361 tgcgctggag gccgccggcg tcgaggtggc cccgctgcgg ccgtcaaacg ccggtttcaa
      421 cgtcgtctgg tccgacatcc tgatcgacaa ggccgccggt caggcgctca agcccgtcgg
      481 gctttcgttc ggcgagcaga ccctcaaggg cgaggtgatg ctgacccgct atggcgtcga
      541 aggcggcggc gtctacgccc tgtccgctgc cttgcgcgag gccatcgccc gtgatggcga
      601 ggccatcctg atgctggacc tgcgccccga cctgaccgaa gccgcgctgg ccgaacggct
      661 gggtcgcccc cgtggcaagg actcgatgac gaaccacctg cgcaaggccg gcggcctgtc
      721 ggcggcggcc atcgccgtcc tgcgcgacat ccccggcgag atgccggcgg gcgccgacaa
      781 gctggccaaa cgcatcaagg gcgtgcggct gaagctgacc gggatgcagg gaatcgaacg
      841 cgccatctcc accgcaggcg gcgtgacgtt gggcgcggtg aatcaacgct tgatgctgac
      901 cgcccgcccc ggcgtgttcg tcgccggcga gatgatcgac tgggaagccc ccaccggcgg
      961 ctacctgctg caggccagtt tcgcctcggg cgtcgtcgcg gccaatggcg tgctggactg
     1021 gctcaaggcg aaatcgtccg ttacggaatc cgcatgacca atctgctcga catctcaggc
     1081 atctgcccgc cgcaattctc ggccgtgaag gacgccttcg ccgccaactt caccgacgcc
     1141 cccgagggcc tgaacgaaca ggcggcgcgg attgcgttca ccgtcgaagg cgaggtcgtc
     1201 gtcgatctct gggcgggtca ggctgacccc cgcgccaata cgcccttcac cgacacgacg
     1261 ctgacgcccg tgttctcgac cggcaaggcc gtcatggcgc tgttgatggc gaccgccgtc
     1321 gagcatggcg acctggtcta tgacgagacg gtcgcgaccc tgtggcccgc ctacggcgcg
     1381 gcgggcaagg ccagcgtcac ggtggcccag atgctgtccc atcaggacgg cctgcccggc
     1441 ttcgacgaag aggtcgaccc ggccatctgg ttcgaccgtc aggccgtgct cgaccgcctg
     1501 gcggcgcagg ccccgatgtg gccccccggc accgcctccg gataccaccc gatcaccgtc
     1561 ggctatctgg cgggggaagt gttccgcctg acagacggac ggaccttggg cgcggccctg
     1621 cgtcaggatt tcgcgacgaa gttcggtctc gacctgtgga tcggcctgcc ggtcgaggag
     1681 cacgaccgcg tcgcctccat ccgcaaacca gcggcgggcg ccagcctcgg cgccgtcgac
     1741 ccgatcaaga ccgccgcttt cctcgccaag ggatcggcgc cgggcggacg cggctcgacc
     1801 gaatggcgga cggcggaaat cccctcggcc aacctgcacg gcacggcccg cgatctggcg
     1861 cgcatcctca gcctcgtcgc caacggcggg cttctcgacg gcgagacggt tctgtctgcc
     1921 gacgttctgt cgcaactgac ccgcgagcgc atccacggtc aggacaaggt cctgccctac
     1981 gagatcagct gggcggcggg gctgatgcgg aacgagggtc tgaacatctt cggtcccaac
     2041 ccggataccg tcggccattg cggctggggc ggctcctgcg tcttcgccga cgccacggcc
     2101 aaggcgtccg gcgcctatgt catgacccgc cagtcgtcgc atctggtcgg ggacccccgg
     2161 gcgacccgac tgatcaacgc cttctacgcc gcgctctgag gcgtccgaag ggtgaagaat
     2221 gagcgaggag cgggcgctcc tggcatcagc aggccgcgtc gactgcggtc ttgatcgcct
     2281 gccgctcggc atgcttacgc caaagggcgg ccagcgcgaa caccacagcc cccgcccaga
     2341 tgaacacgaa cgaggcgatc cgcagcggcg tcaggggctc gcctgacgcg accccgatgg
     2401 cgaactgcag cgtcggggcg aggaactgca ggaagccgag cgtggacagc ggcaaccgcc
     2461 gggccgacca tgcgaacagg gcgagcggca ggaccgtggc cagaccgctc gacaccgacc
     2521 acgcccagtg gcccgcgccg gtgaacccga ccatctgccc ggtccgggct agccagatca
     2581 ggatgacgac gcccaccggg atcatcatga gacattcgac gaacaggccc gtctgagcgt
     2641 cggcggcgac cttcttgcgg ataattccat aggccgcgaa tgtcagggcg atgaggatgg
     2701 acaaccacgg cgcgcggccc agagcgacag tctgcaaaag cacgccgacc gcagccgaac
     2761 cgatggcgat ccagccccag cggtcgatct tctcgcggaa cagcaccatg cccgcgacca
     2821 tgttgatcag cgggttgatg tagtagccga ggctggcctc gatggtgtgg tgatgggtgg
     2881 tcgcccagac ataggcgccc cagttgttca ggatcatagc cgtcgacagc gccagccagg
     2941 ctactgtctt gggcgtggtc agcactcgtc ggacctgagc cgactggccc gacagcatca
     3001 ccaacgccgc cgcgaacagc accgcccaca gaccgcgctg agccaggatc tcgcccgccg
     3061 tgaagccgct cctggccatg gccatgaaca gcagcgggaa caccccccac gccgtgtagc
     3121 aaaccagccc cgcaatcagg gcgttgcggg tttctgtcgc ggcgggggag gccattgcag
     3181 ttcctttccc tctcccgacg ggagagggat tgaccgatca gaccgtgatc gaacggtagc
     3241 cagtcgagga tttgatcgtg cccgtttcgt ccagcagctg ggcgatctgg acggcgttca
     3301 gggccgcgcc cttgcgcagg ttgtcggaca cgacccacat gaccagaccg ttctcgaccg
     3361 tgtcatcctt ccggatgcgc gagacataga cggcgtgctc gccggcggcg tcgaccgggg
     3421 tgatgtagcc gtcgtgctcg tgcttatcga ccaccagaac gcccggggct tcacgcagga
     3481 tggcacgcgc ttcctcgacc gagatcgggc gatcgaactc gaccgtcacg gcttcggagt
     3541 gacccacgaa caccggcacg cggacgcagg tgaccgtcag cttgatgttg ggatcgagca
     3601 tcttgtgcgt ctcgtccgac atcttctgct cttcgtcggt gtagccgtcc tcgttgaacg
     3661 aaccgatgta agggatgacg ttgaacgcga tctgcttggg gaatttcttc ggcgaggcgt
     3721 caccgaggcc gtagatggcc ttggtctggt tccacagctc gtccatgcct tccttgcccg
     3781 cgccggacac cgactgatag gtcgagacca cgacgcgctt gatcttggcg gcgtcatgca
     3841 gcggcttgag cgccacgacc agctgggccg tcgagcagtt cgggttggcg atgatgttct
     3901 tcttgtgcgc gtatttgatc gcgtcggggt tcacctcggg cacgatcagc ggcacgtccg
     3961 ggtccttgcg gaaggcggac gagttatcga tcaccatcgg gccgagcttg ccgatcttct
     4021 cggaccaggc ccgcgacaca tcgccgcccg ccgacatcag cacgatgtcc acggtggtga
     4081 agtcgaaggt ctcgatgtcc tcgcatttga tggtcttgtc gccccaggcg acgtccatcc
     4141 ccttggattt tcgcgaggcg atcgcatgca ttttatcgac ggggaagttc agttcctcga
     4201 ggatgttcag catttcccgg ccgacattgc cggtagcgcc aacgatggcg acgcgatagc
     4261 ccatgatggt cttccttagt gcgctagcgc ttgcgcccct ttcaaaaaga ttggggtggc
     4321 tcgctgcttg agcgcggttg atgcgcgcaa ggcctcgcgt gcgaggttgc ttgagcgcgt
     4381 ttctgcttca ccgcacgatg gttcctgcgg cagggccacg gatgtagtgg ttcgcaggcg
     4441 cgaagcaatc gcttttcaca tcgcgcacgc aaggatatgc cccggtttaa tgacctcccg
     4501 cattcgctcc gcttatgaca tccgcatcga agagggggtt ctggcccccg acccggccca
     4561 ggctccgctg atcgaggcga tggaacggct cgagacggat ctggccaaga agggtctgtt
     4621 cggcggccag cccgaggtcc gggggcttta catctggggc cctccgggac gcggcaagtc
     4681 catgctgatg gacctgttcg tctcggccac gcctgaaccc cgcaagatgc gcgctcactt
     4741 ccacgctttc atggcctggg tccacgatct ggtccggcaa tggcgcgaag gcgacaagaa
     4801 gacccggcag gcggtgttcg gcacccacaa gggcgacgac cccatcgagc cgatcgcgac
     4861 cctgatcgct tcggaggccc ggctgctgtg ctttgacgaa cttcaggtca ccgatatcgc
     4921 cgacgccctg atcctcggcc gactgtttga cgccctgttc gagcgcaagg tggtgctggc
     4981 ggtcacgtcc aaccgcgccc cgaccgagct ctacaagaac ggcctgaacc gccagctttt
     5041 cacccccttc atcgccgaaa tcgaaaagcg ctgcgtcgtg atcgagatcg acggcgcgcg
     5101 cgactggcgg ctcaaccggc tgatgggctc gccggtctgg ttcgtcggtg aggcaggcag
     5161 ggccccgttc gaggcgctgt ggaccgacct gcgcggcggt gaaccggaat cgcctgctca
     5221 cctcaaggtt ctgggccgcg atgtggtcat cgagcgctcg gcgggcagta tggcgcgtgc
     5281 gaccttcgac gaactgtgcg caaagccgct cgggccgcaa gattatctcg ccatcgccga
     5341 acgcttccac accctgttcc tcgacgcggt tccagcgttg aaccccgacc ggcgccacga
     5401 ggcccggcgg ctcgtgacgc tgatcgatgc gctgtacgag gcgggccccc ggctggtggt
     5461 tcgcgcagat acccaaccag aggatctcta tcccgctggt gacggcgcgt tcgagttcga
     5521 gcggacggtg tcgcggctgc gggaaatgtc gagcgcctca tggctggagc gatctgctca
     5581 cgcctgagcc caccgagggg ctcacaactt cgcctcaata gcggctaggc tggcccaacg
     5641 ctggaggaat tcccatgacc gttctcgccg ccgccctttc ggcgttgatg ctctccatga
     5701 tcccggccac cgggcagacg gctcccgtca cgcccgcatc gcgcccggcc gtagttgcag
     5761 gcgtgtcgcc cgcagccgta gtcgcctggc tgaacacgca aggcgccgtc gccggagcgg
     5821 ttcaggagga gcagggccga aaattcgtgc gggtcgatgc cgacggcttg gcctggcggc
     5881 tgtacttccc atcgtgccag ggcgatcgat gctcggacgt tcagttcacc accggcgtcg
     5941 ctaacgccgc gatcaccccc gacctgatca acggctggaa ccgggaccgc cgcttcctca
     6001 aggccatcta cgaagccccc accgctaccg ctccggcctc ggcagtgatc cagtacgacg
     6061 tgttgctgaa cagcggcgga ccggagcaac tcaccgacca cctcgccatc tggcgcagcc
     6121 tcctgcccga gttcgcgcgc ctgaccaccg gcggcgcagc ttcggctccc ccagccgcgc
     6181 cgaagtaagc caaaaagaaa gggccgcagc ttccgccgcg gccccgtcca ttcactcttg
     6241 cgctcaagca gcgcgcgacc gcgtcgcgcg cgttagcgac cttggcattt atgccagcga
     6301 aggctcgatg tccttgcagg cctgcagcag accctggacc gaagcgaccg atttctcgaa
     6361 catcgccttt tcttcgtcgt tggtggtgaa ctcgatgatc ttctcgacac cggcggcgcc
     6421 gatcagggcg ggaacgccga catacaggtc ggacaggccg tactcgcccg acagccagac
     6481 ggcgcaaggc aggacgcgct tctggtcgag caggtaggat ttggccatgg cgatggccga
     6541 ttccgcggga gcgtagaagg ccgaaccggt cttcagcagg gcgacgatct cgccaccgcc
     6601 cttgcgggtc cgcgtgacga tggcgtcgag gtcgtcctgg gtcaggaagc cggccgcgac
     6661 ggcgtcaggc agcggcaggc cgccgaccgt cgagtgacgg accatcggga ccatgtcgtc
     6721 gccgtgaccg ccgagggtcc aggcgttgat gtcctgaacc gagacgccgg ttttttccga
     6781 caggaagtag gcgaaacgcg ccgagtcgag cacgccggcc atgccgacga ccttctcgtg
     6841 cggcagaccc gagaatttct gcagcgccca gaccatggcg tcgagcgggt tggtgatgca
     6901 gatgacgaag gcgttcgggg cgtgggcctt gatgccctcg ccgacagcct tcatgacctt
     6961 gaggttgatc gagaccagat cgtcgcggct catgccttcc ttgcggggca cgccggcggt
     7021 gacgatgcag acgtcggcgc ctgcgatgtc ggcgtaatca ttggcgccct tgagcgagac
     7081 cgacgaaccg aacacggcgg tcgcctcggc gatgtcgagg gctttgccct gcggcgtgcc
     7141 ttgcgcgatg tcgaacagga tcacgtcgcc cagcgcttcg cgcgcagcca cgtgggccag
     7201 ggtgccgccg atcatgccgg cgccgatgag ggcgatcttt gcacgagcca tgggtatctc
     7261 cttggtggcc taacgcgcag tcgcgctatt tgaggccggg ttttgaccgt cagccgagat
     7321 cggcgtcggg tgtttgcagt tgcgagagga aatcgttggg cggcggtcta gacctgtgga
     7381 cgaacctcgg caagcgtccg gaagcgggtg tggggtttac gaccttcgtc ggggctgcga
     7441 gggtgccccc caaagcaaga atgaacggga gtgaagctca tgcgcaaagt caccgcagcc
     7501 ctgatcgtcg cggccatcgg cggcgtcgcg gcgcccgctc tggcccagac ccctcctgcg
     7561 accataggtc agcaatatgt ccccgcgcca tggtggatgc gtgacccggt gatcgcctcg
     7621 atcggaaacg tccgcgtcga gatccaggcc aaccgcgcgg gcttcagcgc caactttcaa
     7681 tccatcgaac gcagcgcggc cgaggcttcg cgcaaagccg ccgatcaggt ccgcgccctc
     7741 agccagtcac tggcggccta tggtgtcgac aaggtccggg ttgagacccg cctgaccacc
     7801 cggccactgt acgaacagta tcgcgacgaa gccgggacgc tgcgcgacaa cgtccgcccc
     7861 gacaagatca accgctacca gacggaggcc tctgtccagc tcaccattcg cgacgtctcg
     7921 gtgctggagc gggtctacgc gacagtcgtc gcgacgcagc cgacctcgat cagcccggtc
     7981 tatttcaatc ttgagcccga gaacgcctgg aagagcaacc tggccgccga ggccatgaag
     8041 gacgcccgcg tccgcgccct cgccgcggcc tccaacgccg gggcgacgct gggctcggtg
     8101 cgcgtgatcg acccgaccgg ccgcgcctgc cagaccgatg tactggccgg ctggccttcc
     8161 tacggttcgg gtgggggtca ggcgacaacc gtcaatgagg atgtggtggt gacggggtcg
     8221 cggatgagaa gtgcgcccat gatggctgct ccgcctgcgc ctccggctcc ccccccccca
     8281 ggtcaggccc cgagtgaaga agcgatcgag gccgcacgac tggcgctgca gccgccgttg
     8341 cagacgctca cggaaacggc ctgcgtggtc tatggattga actgaggcga cgcatctgct
     8401 gcagttgcgc gtcggcgatc gcgacgaaac ggatgtgtga tctggccgag aaaatcctgc
     8461 ggcaccggct catcgacgcc atagagctcc ggcaacgagt ctttcggcat gcggaaagtg
     8521 ccgaacaggt gatcgtagaa cggaaacagc gccgcgaaat tcctgtcgta ggcttcgcgc
     8581 tggtcggcgt gatgccaatg gtggaagcgc ggcgaaccga tcacatgccg caacgggccg
     8641 aagtccaggc tgacgttggc atgcagcagc agggcccata cgttatagac gagcgcatag
     8701 gcgatcagcg gttcgggcga gaaccccagc aggatggccg ggccaacggc ggctgtcgag
     8761 aacagcacct gttcgaccgg atgcacccgg tgggcggcgg cccaatccat atgctcgccg
     8821 ctgtggtgca cggcatggaa acgccagagc agcgggacgg catgggctga ccgatggatc
     8881 gcatagacgc ccagatccga aagcagcaat atctcgaaga actgcagcca gcccggttgg
     8941 gcgcgcatgg cgacgccgac tgcaggcggg atggcgtgat cggccagtac gctgattgcc
     9001 gtgaaggtca gcagcaggca gaacccgata atcgctccgc cgaggaacgt gtgtagcagg
     9061 tcggtggtcc agccgggccg caggatgggc tgagcccggt cgagcggcat cagcctttcc
     9121 agtacgataa agatcgcgcc gaggccgacg atgatgagca ggttctcaag cgtcatcatg
     9181 cgatgatagg ccgttgtcct tgcaggccga ttaaggaaca ctcgattccg gcatgatcgc
     9241 ccaggatagc cagatgcccc acccttccga gcgcggcctc ttcgataccg atcctcgcct
     9301 gctggagaac tccgtcgccg tcgccggctg ggctctgtgc gacgtacgaa tgatggatga
     9361 cgcccgtttc ccctggctgg tgctcatccc ccgcgtcgcc ggggcaagcg aactggacga
     9421 cgtctccgcg gcggaccgtc ccctccttat ggccgagatg atcagggccg gcgagatcgt
     9481 ccgccggctc ggcgaggcca tgcatctggc ggtggacaag gtgaacacgg cggcccttgg
     9541 caacgtcacc agccagctcc atgtccatgt cgttggtcgc cgtcgcgacg accccttgtg
     9601 gccagatcca gtctggggac gccatgggcg tgcgtccctg ccgcaagccg aactgactcg
     9661 cctggccgat ttcgcggcta gattgtattc acaaccccgg taatgccccg ttctcaggac
     9721 tgcgttacat tcgcatcgaa tgtcaggggt ttccggcctt gtcgaacagc atcttcgatc
     9781 gtgggacaga cacagacagc gccatgggcc gtcttgtcag gaccgcctgc gccctgtttt
     9841 cggcgcccgt cggcctgtgc tccatcatcg tcgacgacaa gatcgcccag aggacgctga
     9901 tcggagcgaa tctggcgagc gcaaccggcc gcttcagccc cacgcgggtt ctcattgccg
     9961 agggccccga cgccctgatg ctgattgagg acagcctgac cgacccgcgg gtcatggacc
    10021 atgaattcgt gaccggtccg gcggctgtgc gtttctatgc tggagcgacc gtgagcgacc
    10081 gggcgggcaa ggctatcggc tcgttcagtg tcatggatac ccggccgcgt cggcccctga
    10141 ccggcgagga ggtcgagcat ctgcgccgct tcgcccgcat ggccggcgac ctatacgcgc
    10201 tcgagcgcga tgaacgggcc agcagcgaca agcgccagac tctggaactt gccgaggcca
    10261 tggccggcgt cgggcatttc agcgtggacg ttgccaccgc cgaggtgacc tggtccgacg
    10321 aggtctaccg catccacggt ctggagcccg gctccgtcga tcccaccatc cattcggcga
    10381 ccggcgccta tcacgaggat gacgccgccg ttttgcgggg gttgatggaa aaggccatgc
    10441 gcaccggcga aggatacgat tccctgctgc gccttaaacg agcggacggc gaggaacggg
    10501 ccacgcgttc caaggcccgt tgcgaaatgg atgccaccgg caaggtcgtc cggctgttcg
    10561 gcgtctttca ggacatcacc gaaccccttc agttccagcg ggcgttgatc gaggctcggg
    10621 acgccgccga ggccgccaat caggcaaagt ccgacttcct tgccaacacc agtcacgaaa
    10681 tccgtacgcc tctgaccagc atcatcggct tctcggcgtt tctgaagtcc agcccgaccc
    10741 tgtccgatcg ggaacgtcat tacgccgatc gaatctcggc cgcatcgact gcgcttctgg
    10801 gcgtgatcaa caacgttttg gattactcca aactcgaggc cgaggccttc gagctcgatc
    10861 aagcgtcgtt cgatccgcga ttgatggccg agggcgccgc ggccatgatg gcagaacaat
    10921 gccgcgagaa ggttctcagt ctcgacattc agatctcgcc tctcgtgcct cgcacgatga
    10981 ccgccgacga aggccgggta cgtcaggtgg tcctcaactt cctgtccaat gcgatcaaat
    11041 tcaccgatgt cggcggcatc gttctcaaac tcgacctggt cggcggacgc ctgaaggtcg
    11101 aggtcgccga tacgggcatc ggcatttctc ccgacggggt cgagcgtctg ttcgagcggt
    11161 tcacccaggg cgattcgtca acaacgaagc tttacggcgg caccggcctc gggctcgcca
    11221 tttcgcgtcg gctggtgctg atgatgggcg gtgagatcgg ggtcgccagc acgccgggag
    11281 acggctcgac tttctggttt gaaatccctc tggatcagcc ctgcgggagg tgcagcgtca
    11341 gatgctggcc gaggaagcct tcttctgaga tagcgccatt ccggcaaact actgacggct
    11401 agatcgtcgg gggcgggccg aatttgccgg cgttgtagtc gctgatggcc tggatgattt
    11461 cctcacgggt gttcatcacg aacggtccgt ggctggcgac gggctcgccg atcggctccg
    11521 catgccccaa caggatgatc gcgtccgagc ctgcctcgat gctcaccgct tcgccgtcgt
    11581 tcagcgcgat caggttccag ggctctaccg ccgttccgcc caccgaaacc tgacccgcca
    11641 cggcatacat gaagacggcg cggccacgtg cgacgggcgt ctcgaacatc ccgcctggtt
    11701 tgaagcgcaa cacggcgaga tggatgtcaa tcaacgactg gatcggggcg gtcacctcgc
    11761 cccagacgcc ggagacgggc tcgaccgtga cccgcccatc atcggtgatg aatgtgggaa
    11821 tgtccgccgc ctgcagtccc acgtaatcgg gcttcgtcat cttcagccgg gacggcagat
    11881 tgacccagag ctggaggatt tccatcgacc cgccctcgcg tttgaagctg gtgggggaca
    11941 gttcggcgtg aatcaggcct gacccggcag tcatccactg tatgccaccg gccttgatga
    12001 tgctctcgcc cccggcggaa tcattgtgcg ccagctcacc ctcgaggatg aaggtgaccg
    12061 tctcgaaacc cctgtgcggg tgcgggccga acggcagccc ctggttgccc ggcggatatg
    12121 tctgggggcc gtggtgattc aggaacagga acggatcgag ctggtccagc cccggaccgg
    12181 gaatgggcct gcgcgtgatc aggtcgccga tgtcgtcgcg ataggcgggg tgttgacggg
    12241 cgacggtgcg gatcaaatga gatgtcatca gccacaccta atgtggctga tgggcgttta
    12301 ccagacgccg ggaaccagcc ggaatttcac tttgccggca tgatcgctgt atgcggcatc
    12361 ctgcatcagg atgcgttcct cggcgaatat ccgcatgact tcgaagacca gcc
//
LOCUS       DASVTM010000408         4593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_519840,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000408 DASVTM010000000
VERSION     DASVTM010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519840"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF633_11260"
     CDS             complement(<1..300)
                     /locus_tag="VF633_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270163.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8471683.1"
                     /translation="MTHKFRALALGGALMAATALTAPAAFAQTSGNAPVQSTIQMIQT
                     QPSLNLSATGEVKIAPDMATITFGVVTEASTAQEAMAQNATRMTQVATALRRAGLA"
     gene            505..580
                     /locus_tag="VF633_11265"
     tRNA            505..580
                     /locus_tag="VF633_11265"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:538..540,aa:Ile,seq:cat)
     gene            637..1809
                     /locus_tag="VF633_11270"
     CDS             637..1809
                     /locus_tag="VF633_11270"
                     /inference="COORDINATES: protein motif:HMM:NF006488.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX8471684.1"
                     /translation="MRFNPVFRNMPTTIFETMSGLAKTHGAINLGQGFPDAPGPEDLR
                     RRAAEAVLNGSNQYAPSRGLPELRAAVAEHYARLQGVDLTFEGMLITSGATEAIAAAI
                     MAVVAPGDEVVVIEPVYDAYVPLIERAGGVAAPVRLSPPEWRITAEQLARAVGPRTRA
                     LMFNNPLNPAGRAFDAEEVATVARFCVQHDLIAISDEVWEHVIFDGRIHHSLLVEPGM
                     AERTIKIGSAGKMFGLTGWKIGFACGSPAVIDPVAKAHQFLTFSTAPNLQTAVADGLA
                     WPEARFKEMRADLQRSRDRLASGLAAGGYVVTASEGTYFLCVDLLASGIALDDQTFCR
                     RMVAEFGVAAIPLSSFMSGGVPGRVVRLCFAKADDVLDEAIVRLAKGRAMMISLTP"
     gene            complement(1810..3396)
                     /locus_tag="VF633_11275"
     CDS             complement(1810..3396)
                     /locus_tag="VF633_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471685.1"
                     /translation="MMMPSVSQLRRLALAIVGMAVAFTGLGPAPAQAQVAARVEAQTR
                     PNFGLLLSPAAGNRSNRSHRRYNYRDHRPDYRPDWRPGDGWGPGYGPSGQANEVIVDC
                     GGNPGSGAIEHAVRRVPPGGTLTIRSSGGACVGWLNIDKPMTVRGAGGYAGLVMLQAP
                     EGLPCISIAPGVRAVIQDLAIASPSAGDAPCIAGDEATVIMERVSVRYVGNESAFLIR
                     GGLLDIRTVSIDARTQSPAILVEDGTLTAVSVNVVQAQSGIEISPGSGGPSLLDSVYL
                     TGEHNPSNFGPRAIGVNVRSRRDIGELSIINSRICGYGEGVAVDGVRVTIQGSRICLA
                     DKGVVVYGGELKLDGSRVRARYVGVIAEAGRAVVTDTSFSGVDQVFDAGPRGDIDADH
                     NRLWSRSEICAPELRDEYQGRFSPRWEGGRRHDDRFSCMTRPYPREWWAEEEGALGVP
                     YANDAYNMPQLARFNAGCGWYDSRGGFIDDTRYYGEARWGRRNPPMPYANDPRYDRGW
                     VRDQERQRDRSCSVAHSGGF"
     gene            complement(3411..>4593)
                     /locus_tag="VF633_11280"
     CDS             complement(3411..>4593)
                     /locus_tag="VF633_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8471686.1"
                     /translation="AAARATDKNIEDPIESSVWTGMALANDQGYSPINRAERRGVFGR
                     LRSLSRYDDCRAWERHEGYNRLDAQLSRMSRDEQEEVRDRMIYVLSTQDAAGFRTLAR
                     LHDSLFGPFGEPEDNPEGREAFGRKDGANREERWRGTRASAVNLFQRNDVDAYLYNYM
                     AVQTGDVGAYVLLKDFERSSPSRAASGAFVEAKARQWVPPFEFYPNDAPASGVPFSDE
                     SRPRGDAYDYALSRMQAEMPFVHVGRALRYLGVVQNAPLQPNQLTKHEIESFEAMLGH
                     PMDSYLSPLEKVRAIQLAAMNGSSEAQMVLAVMYSEGIGLPADYARAFHWYSLAEKQG
                     NPEAKFAMSTFFALGVAGVADQDKAEAVAYRIDSALAGFGPSASRLQAVLAQVSRSPR
                     R"
BASE COUNT          763 a         1461 c         1584 g          785 t
ORIGIN      
        1 ggccagaccg gcgcggcgca gagcggtggc cacctgcgtc atacgggtgg cgttctgggc
       61 catggcttcc tgagccgtcg acgcctcggt gacgacgccg aaggtgatcg tggccatgtc
      121 cggtgcgatc ttgacctcgc ccgtggcgga cagatttagc gacggctgcg tctggatcat
      181 ctgaatggtg ctctggaccg gagcgttgcc ggacgtttgc gcaaaggcgg caggcgcagt
      241 cagggcggtg gcggccatga gagcgccacc gagcgccaga gcacggaatt tatgggtcat
      301 ctgaagtctc ctgagatcga accgtcgacg gcgcggaaat ccacgaccac cttggctgtt
      361 cctatgaacg ttcacacctg aacgatgccg acagcgttac agtcaggctt cgttcatcag
      421 cgttacatcg acgacaggac cacggcgtac gtgtggcgct ttgcaaccga cgcatcccct
      481 gctaggccct ccctccgacc ttgggggcga gtagctcaat ggttagagcc gaccgctcat
      541 aacggtctgg ttgggggttc gagtccctcc tcgcctacca aagtccggcc tcgcgcgctg
      601 cttgcgcgac ggcccgacat cggctccgct gcggccatgc gcttcaaccc tgtcttccgg
      661 aatatgccga ccacgatctt cgagactatg tcgggtctgg cgaagacgca tggcgcgatc
      721 aatctgggcc aaggatttcc cgatgcgccc gggcctgagg atctgaggcg acgggcggcg
      781 gaggcggtcc tcaacggctc taaccagtat gcgccatcgc gcggtctgcc cgagctgcgc
      841 gcggcggtgg ccgaacatta tgcacggctg cagggggtcg atctcacgtt cgagggcatg
      901 ttgatcacct ccggggcgac cgaggccatc gcggcggcga tcatggccgt ggtggcgccc
      961 ggcgacgagg tcgtggtgat cgagccggtc tatgacgcct atgtgcctct gatcgaacgg
     1021 gcgggcggcg tcgcggcgcc ggtaaggctg agcccgccgg aatggcggat cacggcggaa
     1081 caattggctc gggcggttgg gccgcgcacc cgggcgctga tgttcaacaa tccgctcaac
     1141 cccgcaggcc gcgcattcga tgcggaagaa gtggcgaccg tggcgaggtt ctgcgtccag
     1201 cacgacctga tcgcgatcag cgacgaggtc tgggagcatg tgatcttcga cgggcggatt
     1261 caccactccc tgctggtcga acccggcatg gcggagcgga cgatcaagat cgggtcggcg
     1321 ggcaagatgt tcggcctgac cggctggaag atcggcttcg cctgcggatc gccggcggtg
     1381 atcgatcccg tcgccaaggc ccatcagttc ctgaccttct cgaccgcgcc caatcttcag
     1441 accgccgtcg ccgacgggct cgcctggccc gaggcgcgct tcaaggagat gcgcgccgat
     1501 ctgcagcgtt cacgcgatcg gttggcctcg gggctggctg cgggcggcta tgtcgtcacg
     1561 gccagcgaag ggacgtattt cctttgcgtg gatctgctgg cgtccggcat cgctctggat
     1621 gaccagacct tctgccgtcg gatggtcgcc gagttcggtg tcgccgccat ccccctgtcc
     1681 agctttatga gcggcggcgt accgggtcgc gtggtacggc tgtgtttcgc caaggccgat
     1741 gacgtgctcg acgaggctat cgtacgcctc gcgaaggggc gggcgatgat gatcagcctg
     1801 acgccgtaat cagaagccgc cactgtgtgc gacgctgcag gatcggtcgc gctggcgttc
     1861 ctgatcccgg acccagcccc gatcgtagcg cgggtcgttg gcgtagggca tggggggatt
     1921 gcggcgcccc cagcgagcct cgccatagta gcgggtgtcg tcgatgaagc cgccacgtga
     1981 gtcgtaccag ccgcagccgg cgttgaaccg cgcgagttgg ggcatgttgt aggcgtcgtt
     2041 ggcataaggc acgcccagtg cgccttcttc ctcggcccac cactcgcggg gatatggacg
     2101 cgtcatgcag ctgaagcggt cgtcgtggcg ccgtccgcct tcccatcggg gactgaaacg
     2161 cccctgatat tcatcgcgaa gttcgggtgc gcagatctca ctgcgggacc agaggcggtt
     2221 gtgatcagcg tcgatatcac cgcgcggacc ggcgtcgaag acctggtcga cgcctgagaa
     2281 ggacgtgtcg gtcacgactg cacgcccggc ctcggcgatg accccgacat agcgggcgcg
     2341 gacgcgggag ccgtcgagct tgagctcgcc cccatagacc acgaccccct tgtcggcgag
     2401 gcagatgcgc gaaccctgaa tggtcactcg cacgccgtcg acggccacgc cctcgccgta
     2461 gccgcagatg cggctgttga tgatgctgag ctcgccgatg tcgcggcgcg agcggacgtt
     2521 gacgccgatg gcacgcgggc cgaagttcga cgggttgtgc tcgccggtca gatagaccga
     2581 gtccagcagc gatggcccgc ctgaacccgg cgaaatctca atgccggact gggcctgaac
     2641 cacattgacg ctgacggcgg tcagggtccc gtcctcgacg agaatggcgg gcgactgggt
     2701 ccgggcgtct atcgaaacgg ttcggatatc cagcaggccg ccacggatca ggaaggcgga
     2761 ttcgttgccg acgtagcgga cagagactcg ctccatgatc accgtggcct catcgccggc
     2821 tatgcagggg gcgtcgcccg cgctgggcga ggcgatggcg aggtcctgaa tgacggcgcg
     2881 cacgccgggc gcgatcgaaa tgcacggcag gccttcaggc gcttgtagca tgaccagtcc
     2941 cgcatacccg cccgcgccac ggacggtcat gggcttgtcg atgttgagcc agccgacgca
     3001 agctccgcca gacgagcgga tggtcagggt accgccaggc ggaacacggc gcacggcgtg
     3061 ttcgatcgct ccactgccgg ggttgccgcc gcaatcgacg atgacctcgt tagcctgtcc
     3121 gctcggacca tagcccgggc cccagccgtc gccgggccgc caatcggggc gatagtccgg
     3181 ccgatggtcg cggtagttat atcggcggtg tgacctgttg ctgcggtttc cagcggccgg
     3241 gctgagcaaa agtccgaagt tcggacgcgt ctgggcctcg acccgggcgg cgacctgggc
     3301 ctgagccggg gcggggccca gcccggtgaa cgccacggcc ataccgacga tggccagtgc
     3361 gaggcggcga agctgagaga ctgacggcat catcatcgga acatcgcctc ctagcggcgc
     3421 ggcgagcggg agacctgcgc caggacggct tgcaggcggg aggctgaggg gccgaagccc
     3481 gcaagtgccg aatcgatgcg gtaggcgacg gcctcggcct tgtcctgatc cgcgacgcct
     3541 gcgacgccga gggcgaagaa ggtagacatg gcgaatttgg cttcgggatt gccctgcttc
     3601 tcggcgagcg aataccagtg gaaggcgcgg gcgtagtcgg ccggcaatcc gatgccctcg
     3661 gaatacatga ccgccagcac catctgcgcc tcggacgagc cgttcatggc cgccaactgg
     3721 atggcgcgga ccttctccag cggcgacagg taggagtcca tcggatggcc gagcatcgcc
     3781 tcgaaggact cgatctcgtg cttggtcagc tggttcggct gaagcggcgc gttctggacc
     3841 acgccgagat atctgagcgc gcgtccgacg tgaacgaacg gcatctcggc ctgcatccgc
     3901 gaaagggcgt aatcataggc gtccccgcga ggccggctct cgtccgagaa aggcacgccg
     3961 gacgcggggg catcattggg atagaattcg aacggcggca cccactgacg ggccttggcc
     4021 tctacgaacg cgcctgacgc ggcccggctc ggcgacgagc gctcgaaatc cttgagcagg
     4081 acataggcgc cgacgtctcc ggtctgcacc gccatgtagt tgtagagata ggcgtcgaca
     4141 tcgttgcgct ggaacaggtt caccgccgag gcgcgtgttc cgcgccagcg ttcctctcga
     4201 ttggcgccat ccttgcggcc gaacgcctcg cggccctcgg gattgtcctc aggctcgcca
     4261 aagggaccga acagcgaatc gtgcagccgg gccaaggtgc ggaaacctgc ggcgtcctgg
     4321 gtcgacagga cgtagatcat ccggtcgcgg acctcctcct gctcgtcgcg cgacatccgc
     4381 gacagctggg cgtccagacg attgtagcct tcgtggcgtt cccaggcgcg gcagtcgtcg
     4441 tatcgtgaca gcgaacgcag ccgtccgaac acgccccgcc gctcggcccg attgatcggc
     4501 gagtagccct gatcgttggc gagggccatg ccggtccaga ccgagctttc gatcgggtcc
     4561 tcaatattct tgtcggtggc tcgcgcggcg gcg
//
LOCUS       DASVTM010000409         6136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_519986,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000409 DASVTM010000000
VERSION     DASVTM010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519986"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..1865)
                     /gene="hisS"
                     /locus_tag="VF633_11285"
     CDS             complement(339..1865)
                     /gene="hisS"
                     /locus_tag="VF633_11285"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX8471687.1"
                     /translation="MTDTPEAAPIRPEARSPRGFADRRGRDLIAERRIVAKVSEVYER
                     WGFEPLETGAFEYADALGKFLPDADRPNEGVFALQDDPESETPGQWMALRYDLTAPLA
                     RFAAQNWETLPKPFRRYAFGPVWRNEKPGPGRFREFVQCDADTVGSDRPEADAEIIAM
                     ACASLKAAGLADGQAVIRVSNRKLFDGLFDAGGVTDPVQRLTALRAIDKFDRLGWEGV
                     SALLGEGRMDESGDYTKGAKLPLGVAATIEAFLASAGDAELSRAGTLDAVARAGGLGA
                     IGEAALEELAAIDLALASMGVDQASVKFDPTIVRGLEYYTGAVFEAELLLNTTDDKGR
                     PVRFGSIGGGGRYDDLVARFTGQSTPATGFSFGVSRLASALKAAGREDASIRGPVVVI
                     VFSQDDMQAYLDAVSELRNAGIPAELYLGRAGMKAQMKYADRRGAPAAIMLGGDEIAA
                     GQVTIKDLDAGRAMAAGVADNAAWKAARPGQVKVDRRALVETIRRIIEESSINNPPIL
                     "
     gene            complement(1914..3164)
                     /locus_tag="VF633_11290"
     CDS             complement(1914..3164)
                     /locus_tag="VF633_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX8471688.1"
                     /translation="MTDTITSVIERRAAGSGVGPVTAAQRIETLDILRGFAVLGILAV
                     NAMTFAWPGQMDAGATAVPYEMNTANRWAVWCVEVLLHNKCRTLFSMLFGISIFLVGG
                     ERSDKARGAVLRRRLLVLAIFGLLHGLAVWFGDILLLYAWSGVFMMLCRSWSPKRLLW
                     TGISLLGAFSLLELGGEVFSALQPYDAAKAAESLTKQAAETLKIVETVRSGWLGAMTE
                     NLKMWAMVQGMSMILFVIPTVGLMMIGLGLFKSGYLAGRAKTGTYATMLVIGIAITAA
                     FGWYNWLDLIPGRAPPTIYIPRVILDLLAPFVTLAWVALLVLMMRHGLGWMTRTLAPV
                     GRMAFTNYLTQSLIMASLFYMPWGPMLYGKVEPAPLWGIVAGVWLLQLIWSPLWLSRF
                     EMGPLEWVWRCLTYGRRVPLLKQA"
     gene            complement(3252..3821)
                     /locus_tag="VF633_11295"
     CDS             complement(3252..3821)
                     /locus_tag="VF633_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phthalate transporter"
                     /protein_id="HEX8471689.1"
                     /translation="MIPTFERPEDLPGQQRVMWRPTPGARIGASLAAVCWPPLLLTFF
                     IWPPTHWIPGREIDWRLVVLFVGLIAAPTGLWLMGRERDRTGLPASRLGIVWRFMLYG
                     GLLGGALAVLFALIQVIIGAVFSGSVMQALGSTETILLIYGVGGLPVAILVGVSYALW
                     AGLCAAFLAFEPAPEKVRDRLGVMRTDKR"
     gene            complement(3818..4696)
                     /locus_tag="VF633_11300"
     CDS             complement(3818..4696)
                     /locus_tag="VF633_11300"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX8471690.1"
                     /translation="MSTIGTVAIVGAGQMGVGIAHAVALGGYSVRLYDIAATRLPLAL
                     GEIAANLARQVGKGTIAQTEADTALARIRTTDSLADVGSADLVIEAAVEDEGIKKILY
                     AALVPHLAPHTLLASNTSSISITRLASATDRADRFIGLHFMKPAPVMGLVEIIRGIAT
                     SAATYDTAVAFAESLGKTTTNAEDFPAFIVNRILVPMMNEAVFTLYEGVGDVVSIDKA
                     LRLGANHPMGPLELADFMGLDVVLAIMNVLYDGLADSKYRPCPLLVKYVEAGWLGRKS
                     GRGFYDYSGPVPVPTR"
     gene            complement(4876..5178)
                     /locus_tag="VF633_11305"
     CDS             complement(4876..5178)
                     /locus_tag="VF633_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX8471691.1"
                     /translation="MGSAIIWLVNAVISLMVWAIIGTAILSWLFAFDVINHRNRFVNQ
                     LSNFLDAVTRPLLEPFRRIIPSLGGIDVSPIVVLLLLEFARRIFNALAGPSLLMMG"
     gene            5353..5428
                     /locus_tag="VF633_11310"
     tRNA            5353..5428
                     /locus_tag="VF633_11310"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5386..5388,aa:Ala,seq:cgc)
     gene            complement(5675..5920)
                     /locus_tag="VF633_11315"
     CDS             complement(5675..5920)
                     /locus_tag="VF633_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471692.1"
                     /translation="MTAADALAPDHQPTTDFRRYVFPGDARAVLIGHSSPGRVLNEAA
                     DPPQFQASDIPLDSAGRTRAQMLEDLLRSGPCVFAAR"
BASE COUNT         1189 a         2023 c         1924 g         1000 t
ORIGIN      
        1 actatctcga atcagggctg tttctcgcgc tcaagcagcg agcgaccgcg tcgcgagcga
       61 tagcgctcta agcaaaaagg cgctcaggca gcgagcgact gcgtcgcgag cgatagcgcc
      121 ctaggaaaat aggcgcgaaa aagcccgctg cgggggatac atcgcagcgg gctttcacgc
      181 tctctgtgga gcggacgttt cctccccgaa acgccttcgt tcggcaactt tgctttcgcg
      241 atgcgccgtt cgagtgaggt caaatgaccc ggaaccttgg tcggcgtcaa tcagcagtca
      301 tgcgaaaacc ggctgtttta tgagtgatcg tcgataactc ataaaatcgg agggttgttt
      361 atcgacgatt cctcgataat gcgtcgaatg gtctccacaa gggctcggcg atccaccttg
      421 acctgacccg gacgggctgc tttccaggcc gcgttatcgg ctacgccagc ggccatggcc
      481 cggccggcgt ccagatcctt gatcgtaacc tgaccggcgg cgatctcatc acctccaagc
      541 atgatcgctg cgggagcgcc ccgacggtcg gcgtatttca tctgcgcctt catgccggcg
      601 cgaccgagat agagctcggc ggggatgccg gcgttgcgca gctcggacac ggcgtcgaga
      661 taggcctgca tatcgtcctg cgagaagacg atcaccacga cgggaccccg gatcgacgcg
      721 tcctcgcgtc cagcggcctt cagcgccgag gccaatcgcg aaacgccgaa cgagaagccc
      781 gtcgccggcg tcgactggcc ggtgaagcgc gcgaccagat cgtcatagcg accgccgccg
      841 ccgatcgagc cgaaccgcac cggccgcccc ttgtcatcgg tcgtattgag cagcagttcg
      901 gcctcgaaca cggctccggt gtagtattcc agcccgcgca cgatggtcgg atcgaacttc
      961 accgaggcct gatcgacgcc catcgacgcc agggccaggt cgattgccgc cagttcttcc
     1021 agcgccgcct cgccgatcgc gccgagccct ccagcccgcg ccacggcgtc cagcgtcccg
     1081 gcccgtgaaa gttccgcatc gcccgctgac gcgaggaagg cctcgatggt tgcggcgacg
     1141 cccagcggca gtttcgcgcc cttggtatag tcgccgctct cgtccatccg gccttcgccg
     1201 agcagggccg agacgccctc ccagcccaga cggtcgaact tgtcgatggc gcgaagggcg
     1261 gtcaggcgct gcaccggatc ggtgacgccg cccgcatcga acaggccgtc gaatagcttg
     1321 cggttggaca cgcggatcac cgcctgccca tcggccagac ccgccgcctt cagactcgcg
     1381 caggccatgg cgatgatctc ggcgtcagct tcgggccggt ccgagcccac ggtgtcggcg
     1441 tcgcactgca cgaactcgcg gaaacggccc gggcccggct tctcgttccg ccacaccggc
     1501 ccgaaggcat aacgacggaa cggcttgggc agggtctccc agttctgggc cgcgaaccgg
     1561 gccaacggcg ccgtcaggtc gtatcgcaga gccatccact gaccgggcgt ttcagattcc
     1621 gggtcgtcct gaagtgcgaa cacgccctcg ttgggacggt cggcgtcggg caggaatttt
     1681 cccagcgcat cggcgtattc gaatgcgccc gtttccagcg gctcgaagcc ccagcgctca
     1741 tagacctcgg acaccttggc cacgatccgc cgctcggcga tcaggtcgcg accgcgccgg
     1801 tcggcgaacc cccgggggct gcgagcttcg gggcgaatgg gagcggcttc gggcgtatcg
     1861 gtcatgcccg ggcgattaag ccatgcggcc ctgcccggca atgtttgagg cccctaagcc
     1921 tgcttgagca gcggcaccct ccggccatag gtcaggcaac gccacaccca ctccagcggc
     1981 cccatctcga accgcgacag ccacagcggc gaccagatca actgcagcaa ccagaccccg
     2041 gccacgatgc cccagagcgg cgccggctcg accttgccgt acagcatcgg cccccacggc
     2101 atgtagaaca ggctcgccat aatcaggctc tgggtcagat agttggtgaa ggccatccgc
     2161 ccgaccggcg ccagcgtgcg cgtcatccag ccgaggccgt ggcgcatcat cagcaccagc
     2221 agcgcgaccc aggcaagcgt cacgaagggt gcgagcaggt cgaggatgac tcggggaata
     2281 tagatcgtcg gcggcgcgcg ccccgggatc agatcaagcc aattgtacca gccgaacgcg
     2341 gccgtaattg cgatcccgat caccagcatg gtcgcatagg ttccggtctt cgctcgaccc
     2401 gccagatagc cggatttgaa caggcccaga ccgatcatca tcaggccgac cgttggaatg
     2461 acgaacagga tcatgctcat gccctgaacc atggcccaca tcttcaggtt ctcggtcatg
     2521 gcgcccaacc aacccgaccg caccgtctcg acgatcttca gggtttccgc agcctgcttg
     2581 gtcagacttt cagccgcctt ggccgcgtca tagggctgca aggcggaaaa cacctcgccg
     2641 cccagttcaa gcaggctgaa ggcgccgagc agactgatgc ctgtccacag caaccgtttc
     2701 ggcgaccacg accgacacag catcatgaag acgcccgacc aggcgtagag caacagaatg
     2761 tcgccaaacc acacggcgag gccgtgcagc agcccgaaga tcgccaacac cagcaggcgt
     2821 cgccggagca ccgcgccgcg cgccttgtcg ctgcgctctc cgccgacgag aaagatcgaa
     2881 atcccgaaca gcatcgagaa aagcgtgcgg catttgttgt gcagcaggac ctcgacgcac
     2941 cacaccgccc accgattggc ggtgttcatc tcgtagggga cggcggtcgc gccggcgtcc
     3001 atctggccag gccatgcgaa ggtcatggcg ttgacggcca ggatgcccag caccgcgaag
     3061 ccgcgcagga tgtcgagcgt ttcgatccgc tgagccgcag tgacaggtcc gacaccagat
     3121 ccagctgctc gccgttcgat gacagaggtg atggtgtcgg tcatctgttg gcccccaatg
     3181 tcagaggctg acctcgacag tgagaatgcc gaaccgggat cttcgaagca agtcgctctg
     3241 caacgctgaa gtcagcgctt gtcggtacgc atgacaccca gtcggtcgcg gaccttttcc
     3301 ggagccggct cgaaggcgag aaaggccgcg catagccccg cccacagggc atagctgacg
     3361 cccacgagga tcgcgacggg caggccgccg acgccgtaga tcagcaggat ggtttcggtc
     3421 gatccgagag cctgcatgac gctgcccgag aagaccgcgc cgatgatgac ctgaatcagg
     3481 gcgaacagca cagccagagc tccgcccagc aggcccccat acagcatgaa ccgccagact
     3541 atgccgaggc gggatgctgg aagtccggtc cggtcccgct ctctgcccat cagccacaac
     3601 cccgtcggag cggcgatcag gccgacgaac aacaccacca gccgccagtc gatctcgcga
     3661 cccggaatcc agtgggtcgg aggccagata aagaaggtca gtagaagggg cggccagcag
     3721 actgccgcga ggctcgcccc gatccgggcg cccggcgtgg gccgccacat cacccgctgc
     3781 tgaccgggaa gatcttcagg cctctcgaaa gtggggatca tcgcgtcggc accggaacag
     3841 ggccggaata gtcgtagaag ccgcgcccgc tcttcctgcc gagccagccg gcctcgacat
     3901 atttgaccag cagcgggcag ggccgatatt tggagtccgc cagcccgtca tagagcacat
     3961 tcataatggc cagaacgaca tccagcccca tgaagtcggc caactcgagc gggcccatag
     4021 gatggttggc gccaagtctc agcgccttgt cgatcgagac gacatcccca acgccctcgt
     4081 acagagtgaa caccgcctcg ttcatcatcg gcacgaggat ccggttgacg atgaaggccg
     4141 ggaaatcctc ggcgttggtg gtggtcttgc ccaggctctc ggcgaaggcg acggccgtat
     4201 cataggtcgc cgctgaggtc gcgatgccgc gaatgatctc gaccaggccc atgaccgggg
     4261 cgggcttcat gaaatgcagg ccgatgaacc gatctgcgcg gtcggtggcc gaggccagcc
     4321 gcgtgattga aatcgacgag gtgttggacg ccagcagggt gtgcggcgcg aggtgcggca
     4381 ccagcgcagc atagaggatc ttcttgatcc cctcgtcctc tacggcggct tcgatgacca
     4441 ggtcggctga tccgacgtcg gccagcgagt cagtcgtccg gatgcgagcg agcgccgtgt
     4501 cggcctcggt ctgggcgatc gtgcccttgc cgacctgacg tgcgaggttg gcggcgatct
     4561 cgcccaacgc cagtggcagg cgggtggctg caatatcgta cagccggacc gaatagccgc
     4621 ccagcgcgac ggcgtgggca atcccgacgc ccatctgtcc ggcgccgacg atagcgactg
     4681 ttccgattgt gctcatgaag tcgtggtgtt cagcggcgtc tggccagaga ccggcacctt
     4741 aagagggtcg ccggacggtg caaggctgga atcgtgcaat gcggcaacag accctgctgc
     4801 agagcggcgc cggagacacg gtttcgcgtc cgggaagatg ttcccagcaa ccttcagccg
     4861 tatcaaccga ccagcctagc ccatcatcag cagcgacgga ccagccagcg cgttgaagat
     4921 ccggcgcgcg aactccagca gcagcaggac cacgatcggc gagacatcga tcccgccgag
     4981 tgatggaatg atacggcgga acggctccag cagggggcgc gtgacggcgt cgagaaagtt
     5041 cgacagctgg ttcacgaacc ggttgcggtg gttgatcaca tcaaaggcga acagccagct
     5101 caggatggcc gtgccgatga tggcccagac catcagggaa atgacggcat tgaccagcca
     5161 tatgatcgca ctacccacgc cttggactcc tgaaacgacg gaaaccggaa accggtccct
     5221 ctccggcgtc ttctagccga ggtcgcccgg ctcgccaaac gcccataggt cgcaggcttc
     5281 tgcgttcctg cccgagcgtt gacaccgtac ccctcgtccc cgtatgtcgc gggctcgcct
     5341 gaaccctgaa gaggggccgt agctcagttg gtagagcgcg tcgttcgcaa tgacgaggtc
     5401 aggggttcga ctcccctcgg ctccaccagg cggtccgatt taattgtttt tattcagcgg
     5461 gatagctgtt tcggcgcggg tgtaccctaa ccgcgaccct aacctgagtt tttacacatc
     5521 tcttgctcga gcctgtcgat aactagaacg aaacacgaac attcatcttg cggctttggc
     5581 cctctcggag tcagaacggc ttcgggtggc aaaccggaga gcagaaatga atgacggagc
     5641 gttagccgtg atgctggctg agctagacgc tgagttagcg agccgcgaag acacatggcc
     5701 cgctgcgcag caggtcctcg agcatctgag cgcgggttcg cccggcgctg tcgagcggta
     5761 tgtcgctcgc ctggaattgc ggcgggtcgg ctgcctcgtt cagaacgcgt cccggtgacg
     5821 aatgcccgat caaaaccgcc cgcgcgtcgc ccgggaagac atagcgccgg aagtcggtcg
     5881 tcggctggtg gtccggtgcg agcgcgtcgg ctgcggtcat gccgccctga tcgatccgcg
     5941 acccctcttc ggacgcacag aggcttggcc acgggagggg cgatccaacc gcttccgctg
     6001 tgcctgcggc tgccgcgaca gccaggtcag ctacacctcg aacacagcac agcgtgatgg
     6061 acccatcagc tctgccgcgc tggcgctgtg gatctgatgc ggtctcgatg agctatgtga
     6121 agcttgagcg gcgacc
//
LOCUS       DASVTM010000410         7293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_409162,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000410 DASVTM010000000
VERSION     DASVTM010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409162"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2386)
                     /locus_tag="VF633_11320"
     CDS             complement(38..2386)
                     /locus_tag="VF633_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX8471693.1"
                     /translation="MSDLPTPRISDLARSRGPNGVSSEAPDYMAGLNVEQRTAVETTE
                     GPVLVLAGAGTGKTRVLTTRLAHILKLGKARPWELLVVTFTNKAAREMRERITHLIGP
                     SSEGLRWLGTFHSVAAQILRRRAELVGLKSSFTILDTDDQERLLKQLLEAANLDTKRW
                     TPKSLASQIDQWKNRGWTPDKLPSTEDFANGKGQALYAAYQSRLATLNACDFGDLLLH
                     NLTILSKHADIAQEYRDRFKYILVDEYQDTNVAQYLWLRLLTSSTGNVCCVGDDDQSI
                     YGWRGAEVDNILRFEKDFPGAKIVKLERNYRSTSHILGAASGLIASNRDRLGKTLWTE
                     DTSGDKVRVRGVWDGEAESRLIADEIETARRTGQKYKDMAVLVRASFQMRAFEERFVM
                     LAIPYTVIGGPRFFERAEIRDAHAYLRLIQSEDDDLAFERVVNVPKRGIGETSVQKIL
                     SIARHDGVSAMTAVRDLITSDELQARTRTALSTFVRDLDRWRLLAHDTRHWEVTETVL
                     EESGYTDMQKADRVTGQTRLDNLKELVQAMQQFETLQAYLEHVSLVMDLDKGDRDDPN
                     GDGSVQIMTLHGAKGLEFPLVFLPGWEEGVFPSQRSIDEKGEKGLEEERRLAYVGVTR
                     AKSDARISFAANRQVYGRWTTQLPSRFVDELPIAHVEAESDTGYYGASGGMKEAKSRW
                     DDAPSFGSGYSSPGWKRAQSFTSAQPSRPAHARHAVIEGEGRLVATADPKAGSGWVRG
                     ERVFHQKFGYGSVRVIEGNKLTVEFEKAGEKKVIDTFVEKAE"
     gene            2542..2790
                     /locus_tag="VF633_11325"
     CDS             2542..2790
                     /locus_tag="VF633_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471694.1"
                     /translation="MSDNMNNGANNTGSNNGGGNSGGGNTGMAFIVGGLLVLVAIIAY
                     FVFARGGMEPAKKDIDVDVNLPKVEAPRLPDAPSPQTN"
     gene            complement(2809..3006)
                     /locus_tag="VF633_11330"
     CDS             complement(2809..3006)
                     /locus_tag="VF633_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471695.1"
                     /translation="MIAEATASARTGAEQFAKDSKTPLGGIRQATQGSFEILARDDIG
                     DETSSLNKKVRVVTTVTYQLK"
     gene            complement(3003..3518)
                     /locus_tag="VF633_11335"
     CDS             complement(3003..3518)
                     /locus_tag="VF633_11335"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8471696.1"
                     /translation="MTFDKSLLPSAVFGGLIAVGLIGAGIFVGQGVINARVGDRQVTV
                     RGVSERNVIGDLAVLSLRFTAAGEELPEVQARIDGDLALVRGFLKAQGYPDDIVALGR
                     LEVTDTKSREYVSSEAVPHYILAQTVVVRTNDVNRVAATGRALNDLVRQGVVLDFTPP
                     TYVFTKLNEVR"
     gene            complement(3557..3781)
                     /locus_tag="VF633_11340"
     CDS             complement(3557..3781)
                     /locus_tag="VF633_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX8471697.1"
                     /translation="MLKKRSVVLSGHATSVALEEEFWAVLDAFAEDRKMSRAGFLAWV
                     DETRGRRPLASACRILALEWAAGKHRLTTA"
     gene            complement(3970..4209)
                     /locus_tag="VF633_11345"
     CDS             complement(3970..4209)
                     /locus_tag="VF633_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471698.1"
                     /translation="MSQVTYRIVEHDGGWAYKLGDTFSETYPDHDSARVGAVRASREQ
                     RSPDETAFIEYENTSGQWITERADGHDRPETSVEG"
     gene            complement(4247..4756)
                     /locus_tag="VF633_11350"
     CDS             complement(4247..4756)
                     /locus_tag="VF633_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471699.1"
                     /translation="MLLPHLSALGWFHTLGSLPAVPLALYMLIRHGRITPEAVTGKAW
                     LVFMFIGAISGALVIKDPPGVAITILSLGSLIVGSTIHKATALGGHRDWIQTVALSTA
                     TFTLFLPSLTETLTRLPAGDPIAASPQAPLVVALQLGLVVCLIVGVTLQLLFLKRAPQ
                     VTMAATAPA"
     gene            4983..5393
                     /locus_tag="VF633_11355"
     CDS             4983..5393
                     /locus_tag="VF633_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX8471700.1"
                     /translation="MELTPILVTAVVFGSIVAIILGPGYFKSRERRELQTTVRAAIDK
                     GQPLPPEVIEAMGLEVTKNLSSRTRDIRRGIIWLAVGIGIAAFGLVGEMGWTNDSWGD
                     GPAHIGNGLFGLACIPTTIGLAFIMLSFFNKNKD"
     gene            5421..5978
                     /locus_tag="VF633_11360"
     CDS             5421..5978
                     /locus_tag="VF633_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8471701.1"
                     /translation="MTKSLMDFHDVELAALAAAGGRREFGELVRRHGSAVRALLRRMG
                     AQTSEADDVAQDAFLTAFQRCSEFRGEGTFAAWVKRIAARLYLKRKSKDARYVAEIET
                     EETAPSIDTPGLVDLDEALKSLSETERVCVSLCHGAGMSHPEIAAAMNLPLGTVKSHV
                     KRGLDKLRARLRPESPMLGRVSNVV"
     gene            5968..6387
                     /locus_tag="VF633_11365"
     CDS             5968..6387
                     /locus_tag="VF633_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471702.1"
                     /translation="MSSDEFDPDIERLFARTPHMPDAPLFVADVETKLASSSRVRTLA
                     LTLAGLVGGAVAVSQAVRVNVNLSTSDSPVAGRALGEGIQAASVGTQDAVQSILDQVG
                     LADLSFGSMSGMQLFWITAAALIALAAAGVMKLSQEA"
     gene            6392..7228
                     /gene="panB"
                     /locus_tag="VF633_11370"
     CDS             6392..7228
                     /gene="panB"
                     /locus_tag="VF633_11370"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX8471703.1"
                     /translation="MSSQKQETVRRLTAPDIMKRKGGDPIVCLTAYDAPTATLLDDHC
                     DLLLVGDSVGMVVHGLPNTVGVTMEMMILHGQAVMRGSKRAMVVIDMPFGSYEGAKEV
                     AYDNCARMMKETGAQAVKLESGPTVPETIAYLVQRGIPVMGHVGLRPQAVLTEGAFKA
                     KGKTDDERLRVIAEAEATADAGAFAIVIEGTAEGVAREITETIDKPTVGIGASAACDG
                     QILVTNDMLGMFDWTPRFVRKYADLRGEIDRAVASYASDVKARRFPAEVETYFSKKPA
                     TP"
BASE COUNT         1313 a         2372 c         2310 g         1298 t
ORIGIN      
        1 ggccatgtcg ccaccttagc aaaatccatc gaaatcgtca ctccgccttc tccacaaacg
       61 tgtcgatgac cttcttctcg ccggcctttt cgaactcgac ggtcagcttg ttgccttcga
      121 tcacccgcac cgagccgtag ccgaatttct gatggaagac gcgctcgccg cgcacccagc
      181 ccgagcctgc cttgggatct gcggtcgcca ctaagcggcc ttcaccctcg atgacggcgt
      241 ggcgggcgtg ggccgggcgc gacggctggg cggaggtgaa cgactgcgcc cgtttccagc
      301 ccggcgacga gtagccggag ccgaacgacg gcgcgtcgtc ccagcgggac ttggcctcct
      361 tcatcccgcc cgaggccccg taatagccgg tgtcgctctc ggcctcgacg tgggcgatgg
      421 gcagttcgtc gacgaaccgg ctcggcagtt gcgtggtcca gcggccatag acctgacggt
      481 tggcggcgaa cgaaatccgc gcatccgact tggcccgcgt gacgccgaca taggcgagac
      541 gacgttcctc ctcgaggccc ttctcgccct tctcgtcgat cgagcgctgc gacgggaaca
      601 cgccttcttc ccagcccggc agaaacacca gcgggaactc cagccccttg gcgccgtgca
      661 gcgtcatgat ctgcaccgat ccgtcgccgt tcggatcatc gcggtcgccc ttgtccaggt
      721 ccatgaccag cgagacgtgc tccagatagg cctgcagggt ctcgaactgc tgcatcgcct
      781 gcaccagctc cttgaggtta tccagccgcg tctggccggt gacgcggtcg gccttctgca
      841 tgtcggtgta accgctctct tcgagcacgg tttcggtgac ttcccagtgg cgcgtgtcgt
      901 gggccagcag cctccagcga tcgagatcgc gcacgaaggt cgacagcgcc gtccgagtcc
      961 gcgcctgcag ctcgtcggag gtgatcaggt cgcgcaccgc cgtcatggcc gagacgccgt
     1021 cgtggcgagc gatcgacagg atcttctgca ccgaggtctc gccgatcccg cgtttgggca
     1081 cgttgacgac ccgctcgaac gccagatcgt cgtcctcgga ctggatcaat cgcagatagg
     1141 cgtgggcgtc acggatctcg gcgcgttcga agaagcgcgg cccgccgatc acggtgtagg
     1201 ggatggccag catgacgaac cgttcctcga acgcccgcat ctgaaacgac gcgcgaacaa
     1261 gcaccgccat gtccttgtat ttctggcctg ttcgtcttgc ggtctcgatc tcgtcggcga
     1321 tcagccggct ctcggcctcg ccgtcccaga caccccgcac ccggaccttg tcgccgctcg
     1381 tatcctcggt ccacagcgtc ttgcccagac ggtcgcggtt ggacgcgatc aagcctgagg
     1441 cggccccgag gatatggctg gtggagcggt agttgcgctc cagcttgacg atcttggcgc
     1501 ccgggaagtc cttttcgaag cgcaggatgt tgtcgacctc ggccccgcgc cagccataga
     1561 tcgactggtc gtcatcgccg acgcagcaga cgttgccagt cgaggacgtc agcagccgca
     1621 gccacaggta ctgggcgacg ttcgtatcct gatactcgtc gaccaggatg tatttgaacc
     1681 gatcccggta ttcctgagcg atatccgcat gtttcgaaag aatcgtcagg ttgtgcagca
     1741 acagatcgcc aaagtcgcag gcgttcagcg tcgccagccg ggactgatag gcggcgtaca
     1801 gcgcctgacc cttgccgttg gcgaaatcct cggtcgaggg cagcttgtcc ggcgtccagc
     1861 cccggttctt ccactggtcg atctggctgg ccagcgactt gggcgtccag cgcttggtgt
     1921 cgagattggc ggcttccagc agctgtttca gcagccgctc ctgatcgtcg gtgtccagga
     1981 tagtgaagct cgacttcagc cctaccagct cagcgcgccg ccgcaggatc tgcgccgcca
     2041 ccgagtggaa cgtcccgagc cagcgcagac cctccgacga cggcccgatc aggtgcgtga
     2101 tccgctcgcg catctcgcgc gcagccttgt tggtgaaggt gacgaccagc agttcccacg
     2161 gcctggcctt gcccagcttg aggatgtgcg ccaaccgggt ggtcagcacc cgcgtcttgc
     2221 ccgtgcccgc gcccgccagc accagcaccg gcccctcggt cgtctcgacc gccgtccgct
     2281 gctcgacgtt gagccccgcc atgtaatcag gcgcttcgga cgacacgcca ttggggccgc
     2341 gcgaacgggc gagatcggag atgcgggggg tcggcagatc ggacaaagag cgactcgtac
     2401 agggctggat agccggaaca tagggagtac ggcgggcaaa gtcagtcccg cgcgtgatcc
     2461 ttaccgtcat gcttcaacgg aaccgcagcg cttcggctcc gttcgatggc cacagggatg
     2521 gcaaaggaag ggatttcatc aatgtccgac aacatgaaca acggcgccaa caacaccggc
     2581 tccaataacg gcggcggcaa cagcggcggc ggcaacactg gcatggcgtt catcgtcgga
     2641 ggcctgttgg tcctcgtcgc gatcatcgcc tatttcgtct tcgcgcgcgg cggcatggag
     2701 cccgccaaga aagacatcga cgtcgacgtc aatctgccca aggtcgaggc tccgcgcctg
     2761 cccgacgcgc cttcgccgca aacgaactaa tacagcgctc tcggctgatt atttcagctg
     2821 ataggtgacc gtcgtcacga cgcggacctt cttgttcagc gaggaggttt cgtcgccgat
     2881 gtcgtcacgg gccagtattt cgaacgaccc ctgggtggcc tgacgaatgc cgcccagggg
     2941 cgttttagaa tccttggcga actgctctgc gccggtccgg gccgaggcgg tcgcctcagc
     3001 gatcatcgca cctcattgag cttggtgaac acataggtcg gcggcgtaaa gtccagcacc
     3061 acgccctgcc gcaccagatc gttcagcgcc cggccggtcg ccgccacgcg gttcacgtca
     3121 ttggtccgaa ccaccacggt ctgcgccaga atgtagtgcg gcacggcttc gctcgacaca
     3181 tactcacggc tcttggtatc ggtgacttcc agccgcccca gcgctacgat atcgtctgga
     3241 tacccctgag ccttcagaaa cccgcgcacc agcgccagat cgccatcgat ccttgcttga
     3301 acctcaggaa gttcctcgcc cgccgccgtg aatcggagcg acagcaccgc cagatcgcca
     3361 atcacattgc gctcggaaac gccgcgcacg gtcacctgcc gatcgccgac ccgggcgttg
     3421 attacgccct gacccacgaa gatcccggcg ccgatcagcc ccacggcgat cagcccgccg
     3481 aacacggctg acggcagcag cgatttgtcg aatgtcatgg tgtttccccc gaaactttcg
     3541 ccgacaatgc gctcgctcag gccgttgtca aacggtgttt ccccgccgcc cattccagcg
     3601 ccagaatccg gcacgcagaa gcgagaggcc gccgcccgcg cgtctcgtca acccacgcca
     3661 gaaacccagc acggctcatc ttccgatcct cggcgaatgc gtccagaacc gcccagaact
     3721 cttcttccag agccactgac gtggcgtgtc ccgacaagac gaccgagcgt ttcttcagca
     3781 tctgcgacgt ccagcgggta ggagcgggaa ggccatgcta acgcgcgtcg tctccggctg
     3841 gaatagcccg gaccgcgcct ggcctgggca gctcgtatac agggctcata tctcacacgg
     3901 gcaagcgccc gtgtatcagg ccgcccttcg gtggaagagc gacctgacgg cacgcgctgg
     3961 aagaaaggct tagccctcga cgctggtctc gggacggtcg tggccgtcgg cgcgttcggt
     4021 gatccactgg cccgaggtgt tttcgtattc gatgaaggcg gtctcgtcgg gtgacctctg
     4081 ttcacgcgaa gcgcggacgg cgccgacgcg ggcgctgtcg tggtcgggat aggtctcgga
     4141 gaaggtgtcg cccagcttgt aggcccagcc tccgtcgtgc tcgacgatgc gataggtgac
     4201 ctgggacatg ggacgctcct tgaaggtgta gcccgctgac actgcgtcag gcgggcgccg
     4261 tagccgccat cgtaacctgt ggcgcacgct tcagaaacag caactgtagc gtcacgccga
     4321 cgatcaggca gacgaccagc ccgagctgca aagcgacgac cagcggcgcc tgcgggctgg
     4381 cggcgatcgg gtcgccggcg gggaggcggg tcagggtctc cgtcaggctc ggcaggaaca
     4441 gggtgaaggt ggcggtgctg agggccacgg tctggatcca gtcgcgatgg ccgccgagag
     4501 ccgtagcctt gtggatcgtt gatcccacga tcagcgagcc gaggctgagt atggtgatcg
     4561 cgacgccggg cggatccttg atcaccagtg cgcccgagat cgcgccgatg aacatgaaca
     4621 ccagccaggc cttgccggtg acggcttccg gcgtgatccg cccgtggcgg atcaacatgt
     4681 agagcgccaa gggaacagcg ggcaggctgc ccagagtgtg aaaccagccc agcgccgata
     4741 ggtggggcag aagcatcgcg tcctcccttg atgtcgcttg cgaatgcgca ggccgacgca
     4801 gacaaccttg accgtaaggc gtggaatgtc gaggttcgga acggcgttcc gcgatcaaat
     4861 cggcgcgacc gatcgcctta cgaaagtttc acgtctgaaa ccgcatctgt tcggggccac
     4921 ccacgcctct tgttatcaga aggccgggac atcaggctcc ggcgcaacaa aggaaaccag
     4981 tcatggaact tacccctatc ctcgtcacgg cggtcgtctt cggatcgatc gtcgcgatca
     5041 tcctcggccc cggctacttc aagagccgcg aacgccgcga gcttcagacc acggtccgcg
     5101 cggccatcga caaggggcag cctctgccac ccgaagtcat cgaagccatg ggccttgaag
     5161 tgaccaagaa cctttcatcg cgcacccggg acattcgccg gggcatcatc tggctggcgg
     5221 tcggcatcgg catcgcagcc ttcggcctgg tcggcgagat gggctggacc aacgacagct
     5281 ggggtgacgg cccggctcat atcggcaatg gcctgttcgg gctggcctgc atcccgacca
     5341 ccatcggtct ggccttcatc atgttgagct tcttcaacaa gaacaaggac tgagaacggt
     5401 cacgacaggg ggaaactgtg atgaccaagt ccctgatgga cttccatgac gtggagctcg
     5461 ccgcccttgc ggcggcgggc gggcggcggg agttcggcga gctggtgcga aggcatggct
     5521 ccgccgtccg cgccctgctg cgtcgcatgg gagcccagac gtccgaggcc gatgacgtag
     5581 cccaggacgc tttcctgacc gcgttccagc ggtgttccga gttccggggc gagggcacgt
     5641 tcgcggcctg ggtcaagcgg atcgccgcac ggctgtatct gaagcgcaag tcgaaggacg
     5701 ctcgctacgt cgctgaaata gagacggaag agactgcgcc gtcgatcgac acgcccggtc
     5761 tggtggatct ggacgaggcc ctgaaatcgc tgagcgagac cgaacgggtc tgcgtctcac
     5821 tctgtcacgg ggcagggatg tcccacccgg aaatcgcggc tgccatgaat ctgccgcttg
     5881 gtacggtgaa gtctcatgtc aaacgtggtc tggataaact ccgcgcccgg cttcggccgg
     5941 agtcgccaat gctgggaagg gtgtcgaatg tcgtctgacg aattcgatcc ggatatcgag
     6001 cgtctgttcg ctcggacgcc acatatgccc gatgcgccgc tgttcgtggc tgatgtcgag
     6061 acgaagctgg cttcaagctc acgagttcgc acgcttgccc tgacactggc cggtctggtc
     6121 ggcggggctg tggcggtcag tcaggccgta cgtgtgaacg tcaatctgtc cacgtccgat
     6181 tcccctgtcg ccggtcgtgc gttgggtgag ggcattcagg ccgccagcgt cggcactcag
     6241 gatgcggtcc agtcgatact ggatcaggtg gggctcgccg atctgtcgtt tggctcgatg
     6301 agcggaatgc agctattctg gatcacggca gcggcgctga tcgcgcttgc cgccgccggt
     6361 gtcatgaagt tgtcccagga agcctgatcc catgtcgagc cagaagcagg aaacggtccg
     6421 ccgtctcacc gcgccggata tcatgaagcg taaaggaggc gacccgatcg tctgcctgac
     6481 cgcctatgac gccccgaccg ccacgcttct ggacgaccac tgcgacctgc tgctggtcgg
     6541 cgacagcgtc gggatggtgg tgcatggcct ccccaacacc gtcggcgtga cgatggagat
     6601 gatgatcctg catggccagg cggtcatgcg tggatcgaag agggccatgg tcgtgatcga
     6661 catgccgttc ggctcctatg aaggggccaa ggaagtcgcc tacgacaact gcgcccgcat
     6721 gatgaaggaa acaggcgcac aggcggtgaa gctggaaagc ggtccgacgg tgcccgagac
     6781 catcgcctat ctggtccagc gcggcatccc ggtcatgggc cacgtcggtc tacggcctca
     6841 ggccgtcctg accgagggcg ctttcaaggc caagggcaag accgacgacg agcggctgcg
     6901 ggtcatcgca gaagccgagg cgacagccga cgcgggagcc ttcgccatcg tcatcgaggg
     6961 caccgccgag ggcgtagcgc gcgagatcac cgagaccatc gacaagccga cggtaggaat
     7021 cggcgcctcg gcggcctgcg acggtcagat cctggtgacc aacgacatgc tgggcatgtt
     7081 cgactggacc cctaggttcg tgcgcaaata tgccgatctg cgtggagaaa tcgaccgcgc
     7141 ggtcgcaagt tacgcctctg acgtcaaagc ccgccgtttt cctgccgaag tcgagaccta
     7201 cttctcgaaa aagcccgcca cgccgtaagg ctgcgttgcg gcgcacacgc cgacgctcta
     7261 gggtccggcc ggattgaaat tcagcggcct cag
//
LOCUS       DASVTM010000411         7041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_733022,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000411 DASVTM010000000
VERSION     DASVTM010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733022"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..569
                     /locus_tag="VF633_11375"
     CDS             177..569
                     /locus_tag="VF633_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471704.1"
                     /translation="MSLEAEKIAEGSELEPGDRRAKSERRGKDRRLENTRINRVAPRR
                     SLMRILGAWATAMSFAIFAIVFLATAMIFENTPWIALSVTGIAMVISAFALMLGSIEQ
                     RLIEIRLELMMANGGMRQADRRNAERRT"
     gene            981..2564
                     /locus_tag="VF633_11380"
     CDS             981..2564
                     /locus_tag="VF633_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8471705.1"
                     /translation="MPSLFAGRAIAAEDLVSTLDVGIKKTLADVAMNAAKSAGATYCD
                     VRVGRYLRQFVITREANVENIVNTESTGVGVRVIADGAWGFAATNAMTVDAVAKAAQQ
                     AVAIAKANAPTLTEPVQLAPVTGVGEVSWRTPIVKNSMEVPIQEKVDLLLGVNAAAMG
                     AGADFIRSMLFLVNEQKYFASTDGSYIDQDVHRIWAPTSVTVIDKATGKFRSRDGLSA
                     PMGLGFEYLDGAPSGKTVLPNGVTVYGQSYDMKEDAIASAQQARAKLTAPSVKPGKYD
                     LVLDPSHTWLTIHESVGHPLELDRVLGYEANYAGTSFATMDKLKGHFQYGSDKVNIVA
                     DKLQPGSLGYVGYDDEGVKTKEWDLIRDGKLVGYQAIRDQAHIEGKPASDGCCYADSW
                     SNVQFQRMANVSLESGKDKMSVADMISDVEDGVYIIGDGSFSIDQQRYNAQFGGELFY
                     EIKNGEITHQIEDVAYQIRTPEFWNACAGVCDASDYRLGGSFFDGKGQPAQVSAVSHG
                     SSTARFNGINVINTARSLG"
     gene            2588..3922
                     /locus_tag="VF633_11385"
     CDS             2588..3922
                     /locus_tag="VF633_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8471706.1"
                     /translation="MSILPEAQAKTILTKVVALSTADECIAQLSGSTRGNIRFALNNV
                     STSGIVDNVELSVQVAFGKRVGTAQINQFDDASLERVVRRAEELAKLAPENPEFMPAV
                     GPQTYTATPTFSAATAAITPEFRSKVAADSIGPCKEANLIAAGYLEDVAGFVAFMNSK
                     GNYGYQTSTSADYTCTVRTADGRGSGWVGDNVQDVANFQAGADVRIAMRKASASAEAM
                     ALEPGKYTVILEPAAAAGLIGFMMNFFDARSADEGRSFLSKAGGGNKTGEQVFGAEVN
                     FFTDPSYPDAAVYPWDGDGLARTRMPIVENGKISNLEYSRYWAQKQGKTAMAGPGNLI
                     MSGGDKSTADLVRTTERGILVTRTWYIRMVDPQTVLLTGLTRDGTFYIENGQIKHPVK
                     NFRFNESPVIMLNNVEELGRPVRVKSDEGDTTMILPPMKLRDFTFTSLSDAV"
     gene            3957..4670
                     /locus_tag="VF633_11390"
     CDS             3957..4670
                     /locus_tag="VF633_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX8471707.1"
                     /translation="MNRAQFLRLGAGGLAGLALSALPRFAAASAAGSYDFWFTRLRYD
                     SGDWDVDQRMPSNLITSLIDYTTLRVDPAERVIPLSDPRMLTAPFCYLAGHKLVEFSP
                     AERRNFERYVRNGGFVFVDDCNHDIDGLFARSFETQMRSIFGPAALKTLPKTHPIYRS
                     FFTFDDGPPNTGLELNGWGDDLVHDYLKGIEINGRLGLLYSNKDYGCEWDYDWRNKRF
                     LAVDNTRFGVNIVMYALSS"
     gene            4714..5712
                     /locus_tag="VF633_11395"
     CDS             4714..5712
                     /locus_tag="VF633_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8471708.1"
                     /translation="MTDTVSEADIQAQLARLGDLRAAIARAIVGQQDVVEQLLIGLLA
                     GGHCLLEGVPGLGKTLLVRSLGEALKLEFRRVQFTPDLMPSDILGTELLEEDHGTGHR
                     SFRFQPGPVFTNLFLADELNRTPPKTQAALLEAMQEHTVSYAGVTHKLNEPFFVLATQ
                     NPLEQAGTYPLPEAQLDRFLLHIRVEYPSEQEERDILAQTTGAKVGAVPAVMTGEEVL
                     ALQALVREVHFSDALLSWVTKIVRTSRPGEGAPEEVRKYVKWGAGPRAGQSLILAAKA
                     RALLQGRLAATREDIVALAAPVMRHRLLLSFAAEAEGRSADDIVAALVRATPMPTT"
     gene            5729..6619
                     /locus_tag="VF633_11400"
     CDS             5729..6619
                     /locus_tag="VF633_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8471709.1"
                     /translation="MTDFIPPDVRSRLKSLRLITRRAVGSHGLGLHQSHSRGAGLEFA
                     QYRAYERGDELRQIDWKLYGRSDKFFVREAERESPVAVWILMDASASMGQVDVGSGAT
                     RFDAAKSLTQSIAELAMQQGDRFGWMALNDQGLRLLPPATGLRQRDRLLLELLPLQPV
                     GVFAREDQLAPVWERIGAHDLVVVLSDCFEPGVVDLAETLAAAGREVLVVEMLTVGEK
                     DFAFNGGYRFRDPETGEELLGDGPALRAEFLRRFGEARSALHARLDATGIRRAEHVLG
                     QPVDLPLRRLFSAHDAAEYS"
     gene            6616..>7041
                     /locus_tag="VF633_11405"
     CDS             6616..>7041
                     /locus_tag="VF633_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX8471710.1"
                     /translation="MTPGLLLPAGLAALAALIVPLVIHIARRSEQQPTDFAALRWLRQ
                     KPRPRSRLRFDEWPLLLLRLLLLALVAFWLARPALFGASDDAPYIAVIPGADLSQADA
                     VIGEGRAHWLAPGSPDLDQPRPAATAPVASLIRQLDADLP"
BASE COUNT         1265 a         2301 c         2248 g         1227 t
ORIGIN      
        1 gacgtcgcaa gggacagcgc ctggacggtg attgtcgcac gatcactgca actcaaggga
       61 gcgccttcgc tcatcatcaa cgccaactac gccacatccg acgtgccggt gcccacaggc
      121 gtcggcccgc gagacggcgg ctcacaattg gtcgactaga aaaagaggca ggggacatgt
      181 cactggaagc cgaaaaaatc gccgaaggca gtgaactgga gccgggcgac cggcgcgcaa
      241 aatcggaacg acggggcaag gatcgcaggc ttgaaaacac ccgtatcaac agggtggcgc
      301 cgcgccgctc cctgatgagg atattgggag cgtgggctac ggcaatgtcc tttgcgatct
      361 tcgccatcgt gtttctcgcg acggccatga tattcgagaa tacgccctgg atcgcgctgt
      421 ccgtgaccgg gatcgcgatg gtgatatccg cattcgcgct gatgctcgga agcatcgagc
      481 aaaggctgat tgagatccgc ctggagttga tgatggcgaa cgggggcatg cgacaagcgg
      541 atcggcgaaa cgccgagcgc cggacctaaa ccacatggcc ggcgagaccg cctggccaaa
      601 agccgccaca gggcgcgacc ggaaatccgt gatccggctc gcgacggctc gacctgcccg
      661 agagcaacgt cacacccgcc acgcgagacg ttgcgactga tggcccttcc aactcgacca
      721 cttcgaatgc ctcaacctga agcttgtctg atcgacggcg gctttatttc ctgtgtgggc
      781 aagctgaaat cgctcttgtc gggaaagatc agcgtttgac ctcgggcgcg tacgcggcaa
      841 tgatcgacat tccggagcct gaggcccggc ccatctatct cccatccaga tcgcgacctg
      901 cacgtttcgc aggcggaggc gaaccaatgc acagacgcga tttcctgtct tttggttcgc
      961 taggagtggg cgggctgctc atgccctccc tgttcgccgg gcgagccata gccgccgagg
     1021 atctggtctc gacgctggat gtcgggatca agaagacgct tgctgatgtg gccatgaatg
     1081 ccgcgaaatc ggccggagcc acctactgcg acgtgcgggt tggccgctat ctgcgccagt
     1141 ttgtcatcac gcgtgaagcc aacgtcgaga acatcgtcaa caccgaatcg accggcgtcg
     1201 gcgtgcgggt gatcgcggac ggagcctggg gcttcgccgc gaccaacgcc atgaccgtgg
     1261 acgccgtggc caaggcggcg cagcaggccg tcgccatcgc gaaagcgaat gcgccaaccc
     1321 tgaccgagcc ggtgcagttg gccccggtga caggcgtggg cgaggtctcg tggcgcacgc
     1381 ctatcgtcaa gaactcgatg gaagtgccga tccaggaaaa ggtcgatctg ctgctgggcg
     1441 tcaatgccgc agccatgggc gccggggccg acttcattcg gtcgatgctg ttcctcgtca
     1501 acgagcagaa gtatttcgcc tcaaccgacg gctcctacat cgatcaggac gtccaccgca
     1561 tctgggcgcc cacgtcggtc acggtgatcg acaaggcgac aggcaagttc cgcagccgcg
     1621 acggcctgtc cgcccccatg ggtctgggct tcgaatatct ggacggcgcg ccttcgggca
     1681 agaccgtcct gccaaacggg gtcaccgtct acggccagtc ctacgatatg aaggaagacg
     1741 ccatcgcgtc cgctcagcag gcgcgcgcca agctgacggc gccgtcggtc aagccgggca
     1801 agtacgatct ggtcctcgat ccctcgcaca cctggctgac catccatgag tcggtgggtc
     1861 acccgctcga gctcgaccgg gttctaggtt acgaggccaa ctatgcggga accagtttcg
     1921 ccacgatgga caagctgaaa ggccatttcc agtacggcag cgacaaggtg aacatcgtcg
     1981 ccgacaagct ccagcccggc agcctgggct acgtcggcta tgacgacgag ggggtgaaga
     2041 cgaaggagtg ggacctcatt cgcgacggca agctggtcgg ctatcaggcc atccgcgacc
     2101 aagcacacat cgagggcaaa ccagcgtccg atggttgctg ttatgcggac agctggtcca
     2161 acgtccagtt ccagcgcatg gccaatgtct cgctggagtc cggcaaggac aagatgagcg
     2221 ttgccgacat gatctcggat gtggaggacg gcgtctatat catcggcgac ggctcgttct
     2281 ccatcgacca gcaacgctac aacgcccagt tcggcggcga gctgttttat gagatcaaga
     2341 acggcgaaat cacccaccag atcgaggatg tggcctatca gatccgcacg ccggagttct
     2401 ggaacgcctg cgccggggtc tgcgacgcca gcgattaccg cctcggcggt tcgttcttcg
     2461 acggcaaggg ccagcctgcc caggtctcgg ccgtatcgca cgggtcttcg accgcgcgtt
     2521 tcaacggcat caacgtcatc aacaccgccc gttcgctcgg ctgatcgcgc accaaggagt
     2581 ctgacagatg agcattcttc cagaggcgca agccaagact atcctgacga aggtcgtggc
     2641 cctgtccacg gccgacgaat gtatcgccca gctttccggg tcgacgcggg gcaatattcg
     2701 attcgccctg aacaacgtct cgaccagcgg catcgtcgac aacgtcgagt tgtccgtcca
     2761 ggtggccttc ggcaagcggg tgggaacggc acagatcaac cagtttgacg acgcctcgct
     2821 ggagcgggtc gtgcgccggg ctgaggaact ggcgaaactg gctcccgaga acccggagtt
     2881 catgccggca gtcggacccc agacctatac ggcgaccccg acgttcagcg ccgcgaccgc
     2941 cgccatcaca cctgagttcc ggtccaaggt ggcggcggac tcgatcgggc cctgcaagga
     3001 agcaaacctg atcgccgccg gctatcttga ggatgtcgcc ggtttcgtcg ccttcatgaa
     3061 cagcaagggc aactacggct accagacctc aaccagcgcc gactacacct gcacggtgcg
     3121 cactgccgat ggtcgcggct ccgggtgggt gggcgacaat gttcaggacg tcgccaattt
     3181 ccaggcgggc gccgacgtgc gcatcgccat gcgcaaggcc agcgcttcgg ccgaggccat
     3241 ggccttggag cccggcaaat acaccgtgat cctcgagcct gcggctgcgg cgggcctgat
     3301 cggcttcatg atgaatttct tcgacgcccg ctcggcggac gaaggtcgca gcttcctgtc
     3361 caaggcaggc ggcggcaaca aaaccggcga gcaggtcttc ggcgccgagg tgaacttctt
     3421 caccgaccca tcctaccccg acgccgcggt ctatccgtgg gacggggatg gcctcgcgcg
     3481 aacgcgcatg ccgatcgtcg agaacggcaa gatctctaac ctcgagtatt cccgctattg
     3541 ggctcagaag cagggcaaga cggctatggc cggcccgggc aatctgatca tgtccggggg
     3601 cgacaagtcg acggcggacc tggtccgcac gaccgagcgc ggcatcctgg tgacccgcac
     3661 ctggtacatc cgcatggtcg atccgcagac cgtgctgctg acgggcctaa cccgcgacgg
     3721 gaccttctat atcgagaacg gccagatcaa acatccggtc aagaatttcc ggttcaacga
     3781 gtcgccggtg atcatgctta acaacgtcga ggaactcggt cgtccggttc gggtgaaaag
     3841 cgacgaaggc gacaccacca tgatcctgcc gccgatgaag ctgcgcgact tcaccttcac
     3901 ctcgctgtcg gacgcggttt gatggccgca gtccggtcga gggcccactc gggggcatga
     3961 accgggcgca gtttctccgg ttgggggcag gggggctggc cgggctcgcc ctgtccgccc
     4021 tgccgcgctt cgctgcggcg tctgctgcgg gtagctacga tttctggttc acgcgactgc
     4081 gctatgactc gggtgactgg gacgtggatc agcggatgcc gtccaacctg atcacttcgt
     4141 tgatcgacta caccaccctg cgcgtcgatc ccgccgagcg ggtcatcccg ctctccgacc
     4201 cccggatgct gacggctccg ttctgctatc tcgctgggca caagctggtg gagttttcgc
     4261 cggccgagcg gcgcaatttc gaacggtatg tccgcaatgg cggcttcgtt ttcgtggacg
     4321 actgcaatca cgacatcgac ggcttgttcg cgcgctcgtt cgagacccag atgcgctcga
     4381 tcttcggccc ggcggcgctc aagaccctgc ccaagaccca tccgatttat cgcagcttct
     4441 tcaccttcga cgacggtccg cccaacaccg gactggagct caacggctgg ggtgatgatc
     4501 tcgtccacga ctacctcaag ggtatcgaga tcaacggccg tctgggcctg ctctacagca
     4561 acaaggacta tggctgcgag tgggactacg actggcgcaa caagcgtttc ctcgccgtcg
     4621 acaatacccg gttcggggtc aacatcgtga tgtacgccct cagttcctga cgccttttcc
     4681 agttcgcctt cttacccctc cccgggacct gccatgaccg ataccgtctc cgaagccgac
     4741 attcaggcgc agctcgcccg tttgggtgat ctccgcgcag ccatcgcccg agccatcgtc
     4801 ggccagcaag atgtggtcga gcaactcttg atcggcctgc tggcgggagg gcactgcttg
     4861 cttgagggcg ttcccggtct cggcaagacc ttgcttgtcc ggtccttggg cgaggcgctc
     4921 aagctcgagt ttcgccgggt gcaattcacg cccgacctga tgcccagcga cattctgggt
     4981 accgaactgc tggaagagga ccatgggacg ggtcaccgca gcttccgctt ccagcccggc
     5041 ccggtcttca ccaacctgtt cctcgccgat gagctgaacc gcaccccgcc caagacccag
     5101 gccgccctgc tcgaagccat gcaggagcac acggtttcct acgccggtgt gacccacaag
     5161 ctgaacgagc cgttcttcgt cctggcgacc cagaacccgc tggaacaggc cggcacctat
     5221 ccgctgccgg aggcgcagct cgaccgtttc ctgctgcaca tccgtgtcga ataccccagc
     5281 gaacaggaag agcgcgacat cctcgcccag acgacaggcg caaaggtcgg cgcggttccc
     5341 gccgtgatga cgggcgaaga ggttctggcg ctgcaggcgc tggtccgcga ggtccatttc
     5401 agcgatgcct tgctaagctg ggtgacgaag atcgtccgca ccagccgccc gggcgagggc
     5461 gcgccggaag aggtccgaaa atacgtcaaa tggggcgccg gtccccgggc gggccagtcg
     5521 ctgatcctgg cggccaaggc gcgcgccctg ctgcagggac ggctggccgc gacgcgcgag
     5581 gatatcgtcg ccctggccgc gccggtcatg cgccaccgcc tgttgctgtc cttcgccgcc
     5641 gaggcggagg gccgcagcgc cgacgacatc gtggccgcac tggtccgcgc cacgcccatg
     5701 ccgaccactt aaatccaaga gccgaggcgt gaccgacttc atccccccag acgtgcggag
     5761 ccggctgaag agcctgcgcc tgatcacgcg ccgggcggtc gggtctcatg gtctgggcct
     5821 tcaccagagc catagccggg gcgcgggcct cgagttcgcc cagtaccggg cctatgagcg
     5881 cggcgacgaa ctgcgccaga tcgactggaa actctatggc cggtcggaca aattcttcgt
     5941 gcgtgaggcc gagcgcgaga gcccggtcgc tgtctggatc ctgatggacg cctcggcctc
     6001 gatggggcag gtggacgtcg gttcgggcgc gacgcggttc gatgcggcga agtcgctgac
     6061 tcagtccatc gccgagctgg cgatgcagca gggcgatcgc tttggctgga tggcgctgaa
     6121 cgatcagggc ttgcggttgc tgccacccgc gaccgggcta cgccagcgcg accggctgct
     6181 gctcgaactt ctgccgctgc aacccgtcgg cgtcttcgcg cgtgaggacc agcttgcccc
     6241 cgtatgggag cggatcgggg cgcacgatct ggtcgtcgtc ctgagcgact gtttcgagcc
     6301 cggggtcgtc gatctggccg agactttggc ggcagccgga cgcgaggtgc tcgtggttga
     6361 gatgctgacc gtgggcgaga aggatttcgc tttcaatggc ggctatcgct tccgggatcc
     6421 ggagactggc gaagagttgt tgggcgacgg cccggccctg cgcgccgagt tcctgcgccg
     6481 gttcggagag gcgcggtcgg ccctgcacgc gcggctcgac gccaccggca tccggcgtgc
     6541 cgaacatgtg ctgggtcagc cggtcgacct gccgctgcgg cggctgttca gcgcccatga
     6601 cgcggcggaa tattcatgac gccgggcttg cttctaccgg cagggctcgc cgcgctcgca
     6661 gccttgatcg tgccgctggt catccacatc gcccggcgca gcgagcaaca gccgacagac
     6721 ttcgccgcac tccgctggct gaggcagaag ccgcgtccgc ggtcccgact gcgcttcgat
     6781 gaatggcctc tgctgctgct tcgcctgctg ctgctggcgc tagtcgcctt ctggctggcg
     6841 cgcccggcgc tgttcggtgc gagcgacgat gcgccctaca tcgccgtcat tccgggcgcg
     6901 gacctgagcc aggcggacgc agtgatcggc gagggcaggg cccactggct ggcgcccggc
     6961 tccccggatc tcgaccagcc ccggcccgca gcgactgccc cggtcgccag cctgatccgt
     7021 cagctcgacg ccgacctgcc g
//
LOCUS       DASVTM010000412        10724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_995069,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000412 DASVTM010000000
VERSION     DASVTM010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995069"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF633_11410"
     CDS             complement(<1..234)
                     /locus_tag="VF633_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471711.1"
                     /translation="MIDAAARLAADLLSCPIALVSVVDEAQQWFKAHIGLDICSTPRD
                     QSFCAIALDLPAHDVLVIENAALDPRFAANPLVV"
     gene            complement(449..1390)
                     /locus_tag="VF633_11415"
     CDS             complement(449..1390)
                     /locus_tag="VF633_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8471712.1"
                     /translation="MNPTLLHNPLRAGVAGVGVMGRNHARVMSEMREFDLAVVFDPDA
                     AVAGNVATAFGAQPLTTAADFVAAGLEAAVVSTPNRHHADLSVALSEAGVHVLVEKPI
                     AATVADAQRMIDAAKANDRVLMVGQVERFNPAVEAVKRAIEGEEVISIQITRVGPFPP
                     RMGEVGVVIDLAVHDIDIIRHLTGSEIVEVQPQLAKTRADREDTALLQFRLENGVIAH
                     ITTNWVTPYKTRTLQVATHNKFVVADLMTRQVTEYFGQQPDGSYSTRGVMSWPAEPLK
                     KELEAFAHAIRTGETPAVTGEDGLRNLEVALRCLGES"
     gene            complement(1394..2551)
                     /locus_tag="VF633_11420"
     CDS             complement(1394..2551)
                     /locus_tag="VF633_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX8471713.1"
                     /translation="MIPFIDLQAQRLRLGGKIEAAVQDAVVAGAWVMGPQVRQFEADL
                     AAFGKAKHALGCANGTDALALPLMAWGIGRGDAVFVPSFTFAATAEVVPWFDAEPVFI
                     DVDARTYNMDAGHLEAAVEATLKEGRLTPKVVIAVDLFGQPADYPAIKAVCDKYGLKL
                     IADSAQGFGCTIDGDHPLKSADVTTTSFFPAKPLGCYGDGGAVLTDDDDLAQLMDSIR
                     VHGKAVANDLKDRTFDHDPKYLNMRVGLNSRLDTIQAAVLIEKLKVFGEEIEWRNRIA
                     KRYNELLAPHVAAVPTVPQGNVSNWAQYTIEHPNRDALAAHLKEQGIPTAVYYPIPLH
                     LQPAYEMYPRGPQGLPVTERLKEVVISLPMHADLDEATQDKVIAAIASFKG"
     gene            2750..3283
                     /locus_tag="VF633_11425"
     CDS             2750..3283
                     /locus_tag="VF633_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8471714.1"
                     /translation="MTTEPTAKSSGVPAVFLDRDGVLNVDSGYPHLEEHLQLIPGAAE
                     AVQLLNRHGYLVVIVTNQSGVARGLFTETQMNAFNTHLVRKLGAKGALIGAVYSAPWH
                     HEATDPAWFHPDHPDRKPNPGMLLRAIADHNIDPARSFMIGDRPSDMEAGRRAGVQSF
                     LFHGPNLYEFVQELLGV"
     gene            3416..4300
                     /locus_tag="VF633_11430"
     CDS             3416..4300
                     /locus_tag="VF633_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8471715.1"
                     /translation="MVASNTGYAERRWTSPDGLALFARDYAPASGPAKLPIIAIHGLT
                     RNSADFEGIAPLLAQSGRRVLAVDVRGRGRSDRAPDAMTYVPDVYARDVLALMEQAGI
                     ERAVFMGTSMGGLITMVLTAMKPKAVTAAILNDIGPQVSPEGLARIAAYSGQPVETPT
                     WAAAAAYAKRINEIAFPLNTDADWDAFARRVFREGTEGTPVLDYDPDIAVPIRAAGAK
                     ALVPNLWPAFRKLARTRPTLLVRGGTSDLLSADIAAKMKKAAPDMEYAEVPGIGHAPM
                     LDEPEAKTAIFEFLAGLP"
     gene            4465..6744
                     /locus_tag="VF633_11435"
     CDS             4465..6744
                     /locus_tag="VF633_11435"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX8471716.1"
                     /translation="MPDQTEKQTFSDQDALDFHRVPTPGKISMAPTKPMATQRDLSLA
                     YSPGVAIPVLAIAKDPDLAYEYTAKGNLVAVISNGTAILGLGNLGHMASKPVMEGKSV
                     LFKRFADVDSFDVEVKTTDPDEFITVVKNIGDTWGGINLEDIKSPECFVIESELQDLL
                     DIPVFHDDQHGTAIISTAGLINAAFVVGKKLEDLKVVLAGAGAAGLSSIGLMKAAGVR
                     AENTVVCDRDGVVYKGREHGMDQWKSVHATDTPLRTLAEAMVGADVVLGLSAKGAITK
                     EMVASMAPNPIIFAMANPDPEITPEDVLSVRSDAIIATGRSDYVNQVNNVLAFPYLFR
                     GALDVRARRVNHEMKIACAKALAALAREDVPDEVAAAYSGRKLKFGPDYIIPTPFDPR
                     LIWYIPPFIAQAAMDTGVARVAIPDMDVYRAALRERVDPSAALMQKISSAVRAAPNKR
                     VVFAEGEETTVIRAAWAFKQAELGTPVLLGREDLIRKNAVEAGLDFDSLGIEIVNARV
                     SHKNVEYTDWLYARLQRRGYLRRDVQRMINQDRNYFAAAMVALGDADAVVSGTTRNFN
                     MVLREVRRVIDVKDRLIGLSIVLAKGRTLFVADTSIHELPDAQELAEIAIQAASAVRK
                     LGRTPRVAFLSHSTFGDPPGERGEKVREAIRILDSMDVDFEYEGEMPPELALDTGARA
                     NYPFMRLSADANVLVMPAIQSASISTRLVQSLGGATVIGPLLVGLDKSVQIVSLGASV
                     SEIITAATFAAYEGAAPEL"
     gene            complement(6772..8529)
                     /locus_tag="VF633_11440"
     CDS             complement(6772..8529)
                     /locus_tag="VF633_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471717.1"
                     /translation="MTDTTSPTKSAGRFGGLAVYGERRSVAMLLLGFAAGLPNLLIFD
                     TLSAWLRDSGVTLEVIGFFALATLTYALKFVWAPLLDRTNIPLLTKWLGHRRSWMLAT
                     QGVIILGLWMISGSNPQTALGSMALFAVIVGFFGATQDIAIDAWRIEVADDNRQGVMA
                     ASYQLGYRVAAITAGAIPLVLAQLYNWNLSYAVMAGMMCFGLLGVLLAPRERQHVIRA
                     IPVGDIPSRPWAERLEWVVRLVLIVLAALIIGTGLTDKPDALLWAANPIVGANGSAAI
                     TAGLADNELSGVLIQFAYVIGGFMVLVGACWPIPGYRTRPGAYLAGSFGAPLIDFFLR
                     FGKLALPILALICLYRLSDFVLNIMNPFYQDLGFDKIEIAEVRKVFGVAMLSLGVFVG
                     GWSVAKFGLIRTMVVGAFMSPLSNLVFAWLATQGHELSALFVAIGVDNIATGYAGTAL
                     IAYMSSLTSIGFTATQYALFSSLYALPGKIVASQSGKIVETAARAADAGGIFAPFKGL
                     FGSMPTGSLVAGAAKSGVSPAGLGAGYFAFFLYSTVIGIFAILLAFYVARRQPAVMAE
                     TRAREEAELAEAATEAQPS"
     gene            complement(8564..10141)
                     /locus_tag="VF633_11445"
     CDS             complement(8564..10141)
                     /locus_tag="VF633_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471718.1"
                     /translation="MARQAKIIDLISDSTDVPPEPSASGGSDQFLRLMSHEMRTPLNG
                     VIGMLGLLTRTRLDGAQRAYAEAAQQSAEHLLGLVNDLLDYARLEAGRLEFDPAPVDV
                     ETLVRGVAELLSPRAHDKGLEIVWSVAGDVPDVMADEGRLRQVLFNLAGNAVKFTDTG
                     AVRIAVERVRGSKARSTLAFVVDDTGPGVPPEARERIFEEFGHVDASDATRFGGAGLG
                     LAVVRRLANAMGGTVTVTDRPHDSAEGLRFPGGSRFRFEAVFESVVGAAPRALPLADL
                     AVAVQSSDPFVRRAAEEQIVASGGLVRNDAAVTLIDHSEAPENGVALLPASGKGVILL
                     KPSERELIGRYRAIGFHGYLIKPLRRASLAERVLAAVQSIEPAIAGAPRRPIAEDDRV
                     TPIRFAGIRVLLAEDNPVGALLAKTLLRREGCVVETAATGDEAVAAMKRARYDMVFMD
                     MRMPGMDGPAATRLIRAGGDRTPIVALTANAFAEDRKTCMEAGMDDHLVKPLEPDALR
                     VALSRWTNRDNRFKVAV"
     gene            complement(10213..>10724)
                     /locus_tag="VF633_11450"
     CDS             complement(10213..>10724)
                     /locus_tag="VF633_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471719.1"
                     /translation="PTMADCSLSEADLAANRTLTFEQFDQYRSTLRSTAWNLSMAGCH
                     AASAEADQDYLIHGPVLTDRQYVVVRWHMALSLAQAGRELEAIPLAAASIAPVEPSED
                     QFDWNTYVRGVWGFLTKRREVLETALTTLKTAPGGRNGINARALGRLSNCFDRPYREA
                     VESGSCAVE"
BASE COUNT         1936 a         3495 c         3509 g         1784 t
ORIGIN      
        1 gacgaccagc ggattggcgg caaaccttgg atccagcgcc gcattctcga ttaccagaac
       61 atcatgcgcc ggcagatcga gggcgatggc gcaaaatgac tggtcgcgcg gggtcgagca
      121 gatgtcgagc ccgatgtgtg ccttgaacca ctgctgcgcc tcgtcgacca ccgacaccag
      181 ggcgatcgga cacgacaaca ggtcggccgc caatcgggcc gcagcgtcga tcacggtgtc
      241 gggctctgcg cccgtcaccc gcagggagcg caggctcgcc aggcgcagct cctcgcgatc
      301 cggggccgaa gcggatgcga tcgaagaaga cgatgtctcg gatgtcatgg tgccgacgcg
      361 cagggggtta ctggcgccag tgtggagcga agatgctacc gctcccttac ggaaacctgt
      421 caccttcgat gatgtcgcag ggacaacctc agctctcgcc cagacaccta agggcgacct
      481 cgagatttcg tagcccatcc tcgccggtga ccgccggggt ttcaccggtg cggatggcat
      541 gggcgaaggc ttccagctcc ttcttcagcg gctcggcggg ccagctcatc acgccgcgcg
      601 tggagtagga cccgtcgggc tgctgaccga aatactcggt gacctgacgc gtcatcagat
      661 cggccaccac gaacttgttg tgagtcgcga cctgcagggt gcgggtcttg tagggggtga
      721 cccagttggt ggtgatgtgg gcgatgacgc cgttctcgag ccggaactga agcagggccg
      781 tgtcttcgcg atctgcgcgg gtcttggcca gttgcggctg gacctccacg atctccgagc
      841 cggtcaggtg gcggatgatg tcgatgtcat ggaccgccag gtcgatgacc acgccgactt
      901 cacccatccg gggcgggaaa gggccgacgc gggtgatctg gatcgagatc acttcctcgc
      961 cctcgatcgc gcgtttgacc gcctcgaccg ccgggttgaa gcgctcgacc tgaccgacca
     1021 tcagcacgcg atcattggcc ttggcggcgt cgatcatgcg ctgagcgtcg gccacggtgg
     1081 cggcgatcgg tttctcgacc agcacatgca cgccggcttc ggacagggcg accgacaggt
     1141 cggcgtggtg gcggttgggc gtggatacga cggcggcctc cagaccagcc gcaacgaaat
     1201 cggcggcggt ggtcaggggc tgggcgccga aagcggttgc gacgttaccg gcgacggcgg
     1261 cgtcgggatc gaagacgacg gcgaggtcga actcgcgcat ctcggacatc acgcgcgcat
     1321 ggttgcggcc catgacgccg acaccggcga cgccggcgcg aaggggattg tggagcaggg
     1381 tcgggttcat ggcttagccc ttgaagctgg cgatggcggc gatgaccttg tcctgggtcg
     1441 cctcgtcgag gtcggcgtgc atcggcaggc tgatgacgac ctccttcaga cgctcggtca
     1501 ccggcaagcc ctgcggtccg cgcggataca tctcataggc tggctgcagg tgcagcggga
     1561 tcggatagta gaccgccgtc gggatgccct gctccttgag gtgagccgcg agggcgtccc
     1621 ggttcggatg ctcgatcgtg tactgggccc agttggagac attgccttgc ggcaccgtcg
     1681 gtacagcggc cacgtgcggg gccagcagtt cgttgtagcg tttggcgatc cggttgcgcc
     1741 actcgatctc ctcaccgaag accttgagct tctcgatcag gacggcggcc tgaatggtgt
     1801 ccagacgact gttcagcccg acgcgcatgt tcaggtattt ggggtcgtgg tcgaaggtgc
     1861 ggtccttcaa gtcgttagcg acggccttgc cgtgcacgcg gatggaatcc atcagctggg
     1921 ccagatcgtc gtcgtcggtc aggaccgcgc cgccgtcgcc atagcaaccc agcggcttgg
     1981 ccgggaagaa gctggtggtc gtgacatcag ccgatttcag cgggtggtcg ccgtcgatgg
     2041 tgcagccgaa gccctgcgcc gaatccgcga tcagcttcag gccgtattta tcgcacaccg
     2101 ccttgatcgc gggataatcg gcaggctggc cgaacaggtc gacggcgatg acgaccttgg
     2161 gcgtaagacg gccttctttc agcgtcgcct cgacggcggc ctccagatgg cccgcgtcca
     2221 tgttgtaggt cctcgcgtcg acgtcgatga agaccggctc ggcgtcgaac cacggcacga
     2281 cctcggccgt cgcggcgaag gtgaaggacg gcacgaacac cgcatcgccg cgcccgatcc
     2341 cccaggccat cagcggcagg gccagtgcgt cggtgccgtt ggcgcagccc agagcgtgtt
     2401 tggctttacc gaaggcggcg aggtcggcct cgaactggcg cacctgtggg cccatgaccc
     2461 aggcgccggc gacgacggcg tcctgcacgg cggcctcgat cttgccgccg agacgaagac
     2521 gctgggcctg gaggtcgatg aaggggatca tggggtatcg cttcttctct ggtgcggccc
     2581 tcgagatcgg gccggagcga cgctgcggcg cctgcccgga aatgcgtccg caaatcaggc
     2641 gaacacgacg atgatggtgc gtcctagcaa atgccgcctc cagacaccaa acacgacagg
     2701 gtcacttcgc gttgcgactt gcaacgccgg acgatagacc ctcgctgtca tgacgactga
     2761 acccaccgcg aaatcttcgg gcgttcccgc cgtctttctc gaccgggacg gcgtgctgaa
     2821 cgtcgacagc ggctatccgc atctggaaga gcatctgcag ttgatccccg gcgcggcaga
     2881 ggccgttcaa ctgttgaacc ggcacggcta tctggtggtg atcgtcacca accagtcggg
     2941 cgttgcgcgc ggactgttca ccgagaccca gatgaacgcc ttcaacactc atctggtgcg
     3001 caagctgggc gctaagggcg cgctgatcgg cgcagtctat tccgcgccct ggcatcatga
     3061 ggcgaccgac ccagcctggt ttcaccccga ccaccccgac cgcaaaccca atcccggcat
     3121 gctgctgcgc gctatcgccg accacaatat tgacccggcc agatccttca tgatcggcga
     3181 ccgtccctcc gacatggagg ccggacgccg ggcgggcgtg cagagcttcc tgttccatgg
     3241 gccgaacctg tatgagttcg ttcaggagct tctgggcgtc tgaacggctc gcgcgttaag
     3301 cgtctcgccg gccacagacc tcgctttttc ggcgaatcgt ccacgttggt ggaatcgcaa
     3361 ttccccggat gttgaattag cctcgtcgtt tcgggggcgt tcagaaggac aagccatggt
     3421 cgcgtcaaat accggatatg ccgagcgacg ctggacctcg ccagatggat tggctctttt
     3481 cgcacgggac tacgccccag cgtccgggcc ggcgaaactg ccgatcatcg ccattcacgg
     3541 cctgacccgg aacagcgccg atttcgaagg catcgcaccg ttgctggccc agagcggccg
     3601 tcgcgtcctc gcggtcgatg tccggggccg gggccggtcg gatcgggcgc ctgatgccat
     3661 gacctacgtc cccgacgtct atgcccgcga cgttctggcc ttgatggagc aggccgggat
     3721 cgagcgcgcc gtgttcatgg gaacctccat gggcggcctc atcaccatgg ttctgacggc
     3781 gatgaagcca aaggcggtca cagccgcaat cctcaacgac atcggcccgc aggtttcgcc
     3841 ggaaggcctc gcccggatcg ccgcctattc tggccagccg gtcgagaccc cgacctgggc
     3901 cgccgccgcc gcctatgcaa agcggatcaa cgagatcgcc ttcccgctga acaccgacgc
     3961 cgactgggac gcctttgccc ggcgggtgtt ccgtgaaggc accgaaggaa ctccggtcct
     4021 cgactacgat cctgacatcg ccgtgccgat ccgcgcggct ggcgccaagg cgttggtccc
     4081 caatctgtgg cccgcgttcc gcaagctggc gagaacccgg ccgacccttc tggtgcgcgg
     4141 agggacgtcc gacctgttga gcgccgacat cgccgcgaag atgaagaagg ccgcgccgga
     4201 tatggagtac gccgaggtcc ccggcatagg ccacgctccc atgctcgacg agcccgaagc
     4261 caaaaccgcg atcttcgaat tcctcgcggg actgccctga ccttccagcg gcattcgggc
     4321 cagcggcatt cgggccagcg gcattcgggc caatagacac cggttacctt gcgtcaatct
     4381 gggcgcagcg gttgttcctg cgctggcgga gccttaggac agagggatct ccctgatccg
     4441 accgacgcgc gcgcaaaggc cctcatgccc gaccagaccg aaaaacagac cttctccgat
     4501 caggatgcac tggattttca cagggtcccg acgccgggca agatctcgat ggcgccgacc
     4561 aagccgatgg ccacccagcg cgatctgtcg ctggcctatt cgcccggcgt ggccatcccg
     4621 gtgctggcga tcgccaagga tccggacctc gcctacgagt acacggccaa gggtaatctg
     4681 gtcgctgtga tctcgaacgg gacggccatc ctcggtctcg gcaacctcgg tcacatggcc
     4741 tccaaaccgg tgatggaggg caagtccgtc ctgttcaaac ggttcgccga cgtcgacagc
     4801 ttcgatgtcg aggtcaagac caccgacccg gatgagttca tcaccgtggt caagaacatc
     4861 ggcgacacct ggggcggcat caatctggag gacatcaagt ccccggaatg tttcgtcatc
     4921 gagagcgagc tgcaggacct gctggacatc cccgtcttcc atgacgacca gcacggcact
     4981 gccatcatct ccaccgccgg cctgatcaac gccgccttcg ttgtcggcaa gaagctggag
     5041 gacctgaagg tggtcctcgc gggcgccggc gcagcgggcc tgtcgtccat cggcctgatg
     5101 aaggctgcag gcgttcgggc cgagaacacg gtcgtctgcg accgcgacgg cgtggtctac
     5161 aagggccgcg aacacggcat ggaccagtgg aagagcgtcc acgctaccga tacgccgtta
     5221 cgtacactgg ccgaggccat ggtcggcgcc gacgtggtgc tgggtctgtc ggccaaaggc
     5281 gcgatcacca aggaaatggt ggcctccatg gctcccaatc cgatcatctt cgccatggcc
     5341 aacccggatc ccgagatcac gcccgaagac gtgctgtcgg tgcgctccga cgccattatc
     5401 gccaccggca ggtcggatta tgtgaaccag gtcaacaacg tcctggcctt cccctacctg
     5461 ttccggggcg cgctcgacgt gcgggcccgt cgcgtaaacc acgagatgaa gatcgcctgc
     5521 gccaaggcct tggcggccct cgctcgcgaa gatgtgccgg atgaagtggc cgccgcctat
     5581 tcgggccgca aactcaagtt cggccctgac tacatcatcc cgacgccgtt cgatccgcgc
     5641 ctgatctggt acatcccgcc gttcatcgcg caggcagcga tggacaccgg cgtggctcgc
     5701 gtcgcgatcc ccgacatgga cgtctaccgg gccgccctgc gcgaacgcgt cgatccgtcg
     5761 gcggcgctga tgcagaagat ttcctcggcc gtacgggccg cgccgaacaa gcgcgtcgtc
     5821 ttcgccgagg gcgaggaaac cacggtcatc cgcgccgcct gggccttcaa acaggctgaa
     5881 ctgggcacgc cggtcctgct gggtcgcgag gatctgatcc gcaagaacgc cgtcgaagcc
     5941 ggtctcgact tcgacagcct cggcatcgaa atcgtcaacg cccgcgtcag ccacaagaac
     6001 gtcgaataca ccgactggct ctacgctcgc ctgcaacgtc ggggctattt gcgccgcgac
     6061 gtgcagcgaa tgatcaatca ggaccgcaac tatttcgccg ccgccatggt cgcgctcggc
     6121 gatgccgatg cggtggtgtc gggtacgacg cggaacttca acatggtcct gcgtgaggtc
     6181 cgccgggtca tcgacgtcaa ggaccggctg atcggcctgt cgatcgttct agccaagggt
     6241 cggaccctgt tcgtggccga cacctcgatc cacgagctgc ccgacgccca ggaactggcc
     6301 gagatcgcca ttcaggccgc ctcggccgtt cgcaagctcg gtcgcacccc gcgcgtcgcc
     6361 ttcctcagcc actcgacctt cggggatcct cccggagagc gcggcgagaa ggtgcgggag
     6421 gcgatccgca tcctcgacag catggatgtc gacttcgaat acgagggcga gatgccgcct
     6481 gaactggcgc tcgataccgg cgcccgggcc aactatccgt tcatgcgcct gtcggcggac
     6541 gccaatgtgc tggtcatgcc cgcgatccag tcggcctcga tctcgacgcg tctggtccag
     6601 tcgctgggcg gcgcgacggt gatcgggccg ctgctggtcg ggctcgacaa gtcggtccag
     6661 atcgtcagcc tcggcgcctc ggtctccgag atcatcaccg ccgccacctt cgccgcctac
     6721 gaaggcgccg cgccggagct ttgagctccg gcgttcaggc gtcgaacgcg gtcaggaagg
     6781 ctgggcctcg gtcgcagcct cggccagttc agcctcttcg cgcgctctgg tctctgccat
     6841 aactgcgggc tgccgcctgg cgacatagaa ggccagcagg atcgcgaaga tgccgatcac
     6901 cgtcgagtac aggaaaaagg cgaaatagcc ggcgccgagc ccggctgggc tcacaccgct
     6961 cttggctgcg ccggcgacga gcgatcctgt cggcatcgag ccgaacaagc ccttgaacgg
     7021 ggcgaagatg ccccccgcat cggccgctcg cgccgccgtc tcgacgatct tgcccgactg
     7081 tgaggcgacg atctttccgg gcagggcgta cagcgagctg aacaaggcgt actgcgtcgc
     7141 cgtgaagccg atcgacgtca ggctggacat ataggcgatc agggcggtcc cggcatagcc
     7201 ggtggcgatg ttatcgacgc cgatcgccac gaacagggcg ctcagctcat gtccctgcgt
     7261 cgccagccag gcgaacacga ggttcgacaa tgggctcatg aaagcgccga ccaccatggt
     7321 ccggatcagg ccgaatttgg cgaccgacca accgccgacg aagaccccca gcgacagcat
     7381 cgccacgccg aagaccttgc ggacctcggc gatctcgatc ttgtcgaatc cgagatcctg
     7441 atagaagggg ttcatgatgt tgaggacgaa gtcggacagc cgatagaggc agatcaacgc
     7501 caggatcggc agggccagct tgccgaaccg caagaagaaa tcgatcagcg gcgcaccaaa
     7561 cgatccggcc agataggcgc cggggcgcgt ccggtaaccc gggatcggcc agcaggcgcc
     7621 gacgagcacc atgaagccgc cgatgacata ggcaaattgg atcaggacgc ccgaaagctc
     7681 gttgtcagcc agtcctgcgg tgatggccgc cgagccgttc gcgccgacga tgggattggc
     7741 ggcccacagc agggcgtcag gcttgtcggt caggccggtg ccgatgatca gggcagcgag
     7801 cacgatcagg accagacgga cgacccattc cagacgctcc gcccagggac gcgaggggat
     7861 gtccccgacg gggatggcgc ggatgacgtg ctggcgctcg cgcggggcca gaagaacgcc
     7921 caacaggcca aaacacatca tacccgccat gacggcgtaa gaaaggttcc agttgtagag
     7981 ttgggcgaga accaacggga tggcgcccgc cgtgatcgcc gcgacccggt agcccagctg
     8041 ataggatgcg gccataacgc cttggcggtt gtcgtctgca acctcgatcc gccaggcgtc
     8101 gatggcgatg tcctgcgtcg cgccgaagaa accgacgatc acggcgaaca gcgccatcga
     8161 gcccagcgcc gtctgcgggt tcgatcccga aatcatccac aatccgagga tgatgacgcc
     8221 ctgcgtcgcc agcatccagg accgccggtg acccagccat ttggtcaaca agggtatgtt
     8281 ggtccgatcg agcagggggg cccagacgaa cttcagcgca taggtcaggg tggcgagggc
     8341 gaagaagccg atgacctcga gcgttacgcc ggagtcgcgc aaccaggctg acagagtgtc
     8401 gaagatcagc aggtttggaa gaccagcggc aaagcccagc agcagcatgg cgacgctgcg
     8461 acgctcgcca tagacagcga ggccgccgaa gcgtccggcg gatttggtcg gagaggtcgt
     8521 atcggtcatc aggggctccg gacgccgtcc cccgaggcag gcgtcagacg gcgaccttga
     8581 agcggttgtc gcggttcgtc cagcggctca gggcgacacg cagcgcatcg ggctccagtg
     8641 gcttgaccag atggtcgtcc atcccggcct ccatgcaggt cttgcgatcc tcggcgaagg
     8701 cgtttgcggt caaggcgacg atcggggtcc ggtctccgcc ggcacggatc aaccgcgttg
     8761 ctgcggggcc atccatgccg ggcatgcgca tgtccataaa gaccatgtcg tagcgggcgc
     8821 gcttcatcgc ggcgacagcc tcgtctccgg tggcggctgt ctcgacgaca cagccctcgc
     8881 gccgcagcag ggtcttggcc agcaaggcgc cgaccggatt gtcctcggcc agaagcaccc
     8941 ggatgcctgc aaagcggatg ggcgtcaccc gatcgtcttc ggcgatcggc ctgcgaggag
     9001 cgccggcgat tgcaggctcg atcgactgga ctgcggccag cacccgttcg gccagagagg
     9061 cccggcgcag gggctttatt agatagccgt ggaagccgat cgcgcggtag cgaccgatca
     9121 gctcgcgctc ggagggcttc agcaaaatca cgcccttgcc agacgcaggc aggagggcga
     9181 cgccgttttc cggggcttcc gaatggtcga tcagggtgac agcggcgtca ttgcgcacaa
     9241 gcccgccgct ggcgacgatc tgttcctctg cggccctgcg cacgaacgga tcggatgact
     9301 gtacggcgac cgcgagatcg gccaggggaa gggcgcgcgg ggcagccccg accaccgact
     9361 cgaacacggc ctcgaaacgg aaacgagatc cgcccgggaa tcgcaggcct tctgcgctgt
     9421 cgtgtggccg gtcggttacg gtgacggttc cccccatcgc gttcgcgagg cggcgaacga
     9481 cggcgagacc gagacccgca cccccgaaac gcgtggcgtc ggacgcgtcc acgtggccga
     9541 actcttcaaa tatccgttca cgggcctcgg gcggaacgcc cggacctgtg tcatcgacca
     9601 cgaaggcgag ggtggagcgg gccttgctgc cccggacccg ctcgaccgcg atgcggactg
     9661 cgccggtatc ggtaaatttg acggcgttgc cagcgaggtt gaacagcacc tgccgcaggc
     9721 gaccctcatc ggccatgacg tctggaacgt cacccgcgac ggaccagacg atctccagac
     9781 ccttgtcgtg ggcgcggggg ctcagcagtt cggcgacgcc gcgcaccagc gtctcgacat
     9841 ccacgggcgc aggatcgaac tccagccgtc cggcctccag ccgtgcatag tccagcagat
     9901 cgttgaccag accgagcaga tgttcggcag actgctgcgc ggcttcggca taggctcgct
     9961 gtgcgccatc aagccgggtc cgggtcagca gacccagcat gccgatcacc ccattgaggg
    10021 gcgtccgcat ctcgtggctc atcagccgca ggaactggtc gctgccgcca gacgccgacg
    10081 gctctggggg gacgtccgtg ctgtcggaaa tgaggtcgat gatctttgcc tgccgggcca
    10141 tgcgcgtctc cgtggagact atcctgcgcg accaagagtt aaggacggtt caagcgtctg
    10201 ggcccaacgc cctcattcga cagcgcaact cccggactcg acggcttctc gataaggtcg
    10261 atcgaagcag tttgacaggc ggccgagcgc gcgggcgttg atgccgtttc gcccgcccgg
    10321 cgcggtcttt agcgtggtca gcgccgtttc gagcacctcg cggcgtttgg tcaggaaacc
    10381 ccaaacgccc ctgacatagg tattccagtc gaactggtcc tcagacggct cgaccggagc
    10441 gatcgaggcg gcggccaacg gtatcgcttc caactcgcgt ccggcctggg ccagactcag
    10501 cgccatgtgc caacgcacca ctacgtactg ccggtctgtc agaaccggcc cgtggatcag
    10561 atagtcctga tcagcctcgg cggaagcggc gtgacatccc gccatcgaca ggttccaggc
    10621 ggttgatcgc agggtcgacc ggtactggtc gaattgctcg aacgtcaggg tgcggttggc
    10681 ggcaaggtca gcttcggaca acgaacagtc cgccatggtg ggtg
//
LOCUS       DASVTM010000413         7034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_838699,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000413 DASVTM010000000
VERSION     DASVTM010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838699"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1254)
                     /locus_tag="VF633_11455"
     CDS             complement(16..1254)
                     /locus_tag="VF633_11455"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8471720.1"
                     /translation="MRLIALASSVSLLVLAAACGARSESEPASAATSAGPQLETRPAN
                     GQGQAPAFAGQTRAPGVTSDVKTASTVVASGLDHPWGLALLPDGRWLVTERAGRLRII
                     TAEGGKDEPITGLPPVDARGQGGLFDVAVGPGFASDRMIYWSYAEPRQGGNATSVARG
                     RLSDDGAKVEQVQVIFRALPTYDGDKHFGGALAFAPDGKLFITLGERSDKPMRPQAQD
                     LGSHMGKVIRINADGSVPSDNPFVGRAGALPEIWSLGHRNVQGVAIRSDGQVWTIEHG
                     TRGGDEVNHDRAGVNYGWPIIAYGVEYAGGQINEGITAREGLEQPVYYWDPVIAPGGG
                     TFYDGAMFPGWRGNLLVAGLKEKHISRLVMEGDKVVGEERLLTDLGERVRDVAVGPDG
                     AVWAITDETNGKLVRLAVAP"
     gene            1459..1535
                     /locus_tag="VF633_11460"
     tRNA            1459..1535
                     /locus_tag="VF633_11460"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1493..1495,aa:Met,seq:cat)
     gene            complement(1670..2140)
                     /locus_tag="VF633_11465"
     CDS             complement(1670..2140)
                     /locus_tag="VF633_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471721.1"
                     /translation="MARKASPEIGEGAVIAIPLSDGRWALSQVYRPGISFFLLVFDQV
                     SDRLEDPLVLTGRPILGSWTNDAEIYRGNWKVLTHRPVAPSIFVEPEYTFIIDGETMV
                     ESFDGTRRRAKAACGDEALRTRSSQSPLLVQDAARAAFGIEAWKPVYDRMRLQP"
     gene            2394..2702
                     /locus_tag="VF633_11470"
     CDS             2394..2702
                     /locus_tag="VF633_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471722.1"
                     /translation="MAIRIPALVVLAALTLGAAACTQGETTEAKSDAAVAGEQVKDAA
                     AQTGEVIESGTAKAANAVSEGAAKVADHLEADQAKDAAAGAPGAVNPHTDERVPAPAE
                     "
     gene            complement(2705..3328)
                     /locus_tag="VF633_11475"
     CDS             complement(2705..3328)
                     /locus_tag="VF633_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8471723.1"
                     /translation="MSGSKTAIGIIAGVLLIGVIGGGAFLYAKPDGAAKVSAPAETPK
                     SALARFATGSLAALRTPAATPVAPDYVFKTADGADARFSQFRGKVVMVNLWAMWCAPC
                     RTEMPTLAALAKSYEGKDLVVLPINVDIGDDALADARSFIGVHEPLPLYNDPKFQLPF
                     EFPGKGKMPQTILLDRQGRIRATFAGEADWNSVEAKALIDAVLAEPA"
     gene            3327..4757
                     /gene="argH"
                     /locus_tag="VF633_11480"
     CDS             3327..4757
                     /gene="argH"
                     /locus_tag="VF633_11480"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX8471724.1"
                     /translation="MTDRSTPEEKTKAAQKPAGQDMWGGRFSSKPADIMQAINVSIGV
                     DQRLWAQDLAGSRAHCRMLIAQGIIGAADGEAILGGLDAIEGEIRAGSFPFRDSFEDI
                     HMNVEARLSELIGEPSGRLHTARSRNDQVATDFRLWVRDACDRSGYQLVALQRALLAR
                     AEQHAADLMPGFTHLQTAQPVTFGHHLMAYVEMFGRDASRFADARKRMNENPLGAAAL
                     AGSPFPIDRHATAAELGFDRPMANSLDAVSDRDFALESLAAASITAGHLSRLAEEIVI
                     WMTPMFGFASLPDDLTTGSSIMPQKRNPDAAELVRAKTGRILGSLVALSTVMKGLPLA
                     YSKDMQEDKPPVFEAFDALDLALTAMTAMVSALIPNTERMAAAAGAGYSTATDLADWL
                     VRELNLPFRRAHHVTGAAVKLAESKGVGLAQLSQAELQLLEPGVTEAIYKVLTPEASC
                     ASRQSFGGTAPEQVRARIADWKLRLS"
     gene            4754..4993
                     /locus_tag="VF633_11485"
     CDS             4754..4993
                     /locus_tag="VF633_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471725.1"
                     /translation="MKKILTLGVLALLATSAAACGRMADLEAPAPRQTERAPRDAWAP
                     NLPDPATVNRPSSQAPIDGGPSNPIGGTAAGRDPQ"
     gene            5001..6293
                     /gene="lysA"
                     /locus_tag="VF633_11490"
     CDS             5001..6293
                     /gene="lysA"
                     /locus_tag="VF633_11490"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX8471726.1"
                     /translation="MHHFELKDGALHAEGVALEALADTVGTPVYVYSSATLTRHYTVL
                     REAADAHRNALGDALIAYAVKANSNLSVLATLARLGCGADTVSEGEIRRALAAGVPAS
                     KIIFSGVGKTDSELAFAITAGVRQINVESPAELDRLIPIAERLGAVPEIAIRVNPKIG
                     AGGHAKITTGGEGDKFGIPADEAMELYVRAAASPHVTPVGLACHIGSQITDLTPLEAA
                     FRLLRSLTEQLRARGQTVTRLDLGGGLGVPYVGGAPTASPADYVAMAARVLDGLDVEA
                     AFEPGRLLAANAGVLLSRVIQITERADGRRFLVLDAAMNDLIRPAMYDSFHEVQPVRP
                     RAFGGHNAPTAYDIVGPICETGDTFARGRDLPPLQAGDLVVFTGAGAYGAVMSSEYNT
                     RPLVPEVLVDGERYAVIRPRPTYEEMLAREPMAEWLQE"
     gene            complement(6369..6914)
                     /locus_tag="VF633_11495"
     CDS             complement(6369..6914)
                     /locus_tag="VF633_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX8471727.1"
                     /translation="MADSSPQMSSTREILLSEADITRVVAELAEKIAPRIDDDTVAAV
                     LLTGGLWFAADLTRALSRVGRHVRFDALWLASYGDEKSSRGRIDVYAPFQRDLKGRKV
                     LILDDVFDTGLSLAEAVRIAKEAGASEVLTCVFARKPFPLPSAAEPDFFGWQAPDRFL
                     VGYGLDNAGALRGLPDICALD"
BASE COUNT         1183 a         2406 c         2257 g         1188 t
ORIGIN      
        1 cggcggcctg ccgtctcagg gcgcgaccgc cagccgaacc agcttgccgt tcgtttcgtc
       61 ggtgatcgcc cagacggcgc cgtccgggcc gaccgctacg tcacggacgc gctctccgag
      121 atcggtcagc agacgttctt cgccgacgac cttgtcgccc tccatcacca gtcgtgagat
      181 gtgcttttcc ttcaacccgg cgaccagcag gtttccgcgc cagcccggga acattgctcc
      241 gtcataaaag gtcccgccgc ccggagcgat gactggatcc cagtaataga caggctgttc
      301 gaggccttcg cgtgcggtga tcccttcgtt gatctggccg cccgcatatt ccacgccata
      361 tgcgatgatc ggccagccat agttcacgcc cgcccggtcg tgattgacct cgtcgccgcc
      421 gcgcgtgccg tgttcgatgg tccagacttg accgtccgaa cggatcgcga ccccctggac
      481 gttccggtga ccgaggctcc agatctcagg cagggcgccg gcgcgaccga cgaacgggtt
      541 atcggacggg accgaaccgt cggcattgat gcggatgacc ttgcccatgt gcgaaccgag
      601 gtcctgagcc tgaggccgca tcggtttgtc ggaacgttcg cccagcgtga tgaacagctt
      661 gccgtcgggc gcaaatgcca gagcgccgcc gaaatgcttg tctccgtcat aggtcggcag
      721 ggcgcggaag atgacctgga cctgttcgac cttggcgccg tcatccgaca gtcggccgcg
      781 cgcgaccgat gtggcgtttc cgccctgacg cggttccgcg taggaccagt agatcatccg
      841 gtcggaggcg aagcccgggc caacggcgac atcgaacagg ccgccctgtc cgcgcgcatc
      901 gacggggggc aggccggtga tcggctcgtc cttgccgccc tcggccgtga tgatccgcaa
      961 acgcccggcc cgctcagtca ccagccagcg accatcgggc agcagggcca gaccccacgg
     1021 atggtccagc cccgaggcaa ccaccgtcga ggccgttttc acatcgctcg tcacgccggg
     1081 cgcgcgggtc tgtccggcga aggcgggcgc ctgaccctga ccgttggccg ggcgggtctc
     1141 gagctgtggg ccagcggacg tcgccgccga agccggttca ctctcgctcc tcgccccaca
     1201 ggcggcggcg aggaccagaa gggagacgct ggaggcgagg gcgatcagac gcatggaaat
     1261 gtaaccttga ggactgtcag agacatatgg ccaagcaacg atagggccgc taccctcgtt
     1321 cccgccgatg tgtcgggcgc tgctgcggct gatcgtctcc actcgcgtcc agacatcact
     1381 tgaacccgcg cccggccctc tatataggca cgcctctccc gcgcgcggcc gtcaggctcg
     1441 ccgggcgacc gatagcgtgg ctggatagct cagctggtta gagcggtgga ttcataaccc
     1501 acaggtcggc ggttcaagtc cgcctccagc caccatcggt ccccacatcg agaaacgccc
     1561 gtcggggctg actggcagcg gtctgatggt ccgctattcg cttcgaagcc aatgaccgca
     1621 atcgacccgt tgcggacgtc agcgacgacg ccgtaactgt cgtcaaacct tagggctgaa
     1681 ggcgcatccg gtcatagact ggcttccaag cttcgatgcc gaaggcggct ctcgccgcat
     1741 cctggacgag gagtggagac tggctgctcc tggttctcag tgcttcgtct ccacatgctg
     1801 ccttggctcg ccgcctcgtg ccatcgaagg actccaccat cgtctcaccg tcgatgataa
     1861 aggtgtattc cggttcgacg aagatgctgg gtgcgaccgg acggtgtgtc aggaccttcc
     1921 agtttccccg ataaatttcg gcatcgttcg tccatgatcc taggattggg cgccccgtca
     1981 ggacgagcgg gtcctcaagc ctgtcgctga cttggtcgaa caccagcagg aagaagctga
     2041 taccaggacg ataaacttga gaaagcgccc agcgtccgtc gctaaggggg atggcgatga
     2101 cagccccctc gccgatttcc ggcgaagctt ttcgcgccat gtcgtctcct gggaagatga
     2161 tacaagctac gcgccgcaag gtccgctttc cacccctggc agacatcggc gaagtccggt
     2221 ctccgccgct tcacgatgca gcgtcagagc ggttggatgc gcaacgtgat ttgccgaaca
     2281 tgttggggca cgacggaacc ggcccccgcc cccacgcgac aaatcgcgcc cgtcgcaacc
     2341 cggcgtcgcc gcccgcgttc tcccgccaag gttcacctcg ccggagacat tccatggcta
     2401 tccgcattcc cgctctcgtc gttctcgccg ccctcactct aggcgcggct gcctgcaccc
     2461 aaggcgagac caccgaagcg aagtccgacg ccgccgtggc gggtgaacag gtcaaggatg
     2521 ccgcagccca gaccggcgaa gtgatcgagt ccggtaccgc caaagctgca aacgcagttt
     2581 cggaaggtgc ggccaaggtc gccgaccacc tggaagccga tcaggccaag gacgccgccg
     2641 ccggggctcc tggcgccgtg aacccgcaca ccgacgaacg cgtgcccgcg cccgccgagt
     2701 agactcaggc tggttcggcc agaaccgcgt cgatcagggc tttcgcttcc acgctgttcc
     2761 agtcggcttc gccggcgaag gtggcgcgga tgcggccctg acggtccagc aggatcgtct
     2821 ggggcatctt gcccttgccc ggaaactcga acggcagctg gaatttggga tcgttgtaga
     2881 gcggcagcgg ctcgtgcacg ccgatgaagc tgcgcgcgtc ggcgagtgcg tcgtctccga
     2941 tatcgacgtt gatcggcaga acgaccaggt ccttgccttc ataggacttg gccagcgccg
     3001 ccaacgtcgg catttcggtg cgacatggcg cgcaccacat ggcccacaga ttgaccatca
     3061 cgaccttgcc gcggaactgg ctgaaccgcg cgtccgcgcc gtcggcggtc ttgaacacat
     3121 agtcgggcgc gacgggtgtg gcggctggcg tccgaagagc ggccaggctt ccggtcgcga
     3181 accgcgccag cgcgcttttc ggcgtctcgg ccggtgcgga aactttggcg gcgccgtcag
     3241 gcttggcgta taggaacgcg ccgccgccga tcacgccgat cagcagtacg cccgcaataa
     3301 tccctatcgc tgtcttcgag ccgctcatga ccgatcgttc cacccctgag gaaaagacta
     3361 aggcggctca aaagcctgct ggtcaagaca tgtggggcgg ccgcttctcg tcgaagcccg
     3421 ccgacatcat gcaggccatc aacgtctcca tcggcgtgga tcagcgtctg tgggctcagg
     3481 atctggccgg atcgcgcgcc cattgccgga tgctgatcgc gcaggggatt atcggcgccg
     3541 ccgacggcga ggctattctc ggcggtctcg acgccatcga gggcgagatc cgcgctggct
     3601 ccttcccgtt ccgcgacagc ttcgaggaca tccacatgaa cgtggaggcc cgcctgtcgg
     3661 aactgatcgg cgagccttcg gggcgtctgc acacggcgcg tagccgcaat gatcaggtcg
     3721 cgaccgattt ccgcctgtgg gttcgcgacg cctgcgaccg ctctggctat cagctggtcg
     3781 cccttcagcg tgcgctgctg gcccgagccg aacaacacgc cgccgacctg atgcccggct
     3841 tcacccatct gcagacggct cagccggtta cgttcggcca ccacctgatg gcttatgtcg
     3901 aaatgttcgg ccgcgacgcc tcgcgtttcg ccgatgcgcg caagcggatg aacgagaatc
     3961 ctctgggcgc tgcggctttg gccggctcgc ccttccccat cgaccgccat gcgaccgccg
     4021 ccgagctcgg cttcgatcgt ccgatggcca actcgctgga cgccgtgtca gatcgtgact
     4081 tcgcgctgga gagcctggct gctgcctcga tcacggcggg ccacctgtcg cgtctggccg
     4141 aagagatcgt catctggatg acgcctatgt tcggcttcgc ctccctgccc gacgacctga
     4201 ccaccggctc ctcgatcatg ccgcagaagc gcaatcccga cgccgccgaa ctggtgcgcg
     4261 ccaagaccgg gcgcatcctc ggttcgctgg tcgccctgtc caccgtgatg aagggcctgc
     4321 cgctggccta ttccaaggac atgcaggagg acaagccgcc agtgtttgag gcgttcgacg
     4381 ccctcgatct cgcgttgacc gcgatgacgg ccatggtttc ggcgttgatt ccgaacacgg
     4441 agcgaatggc tgctgctgcc ggcgcaggct actcgaccgc gaccgatctg gccgactggc
     4501 tggtgcgcga gctcaacctg ccgttccgcc gggcgcacca tgtcacgggc gcggcggtga
     4561 aactggccga gagcaagggc gtgggtctgg cgcaactgtc gcaggccgaa ttgcaattgc
     4621 ttgaaccggg cgtcaccgaa gccatttaca aggtgctcac tcccgaggct tcctgcgcca
     4681 gccgccagag tttcgggggt accgcgccgg aacaggttcg cgcgcgcatc gccgattgga
     4741 aattgcgtct ctcatgaaaa agatcctgac cctcggcgtg ctcgcccttc tggccacttc
     4801 ggctgctgct tgcgggcgga tggccgatct ggaggctcca gcgccgcgcc agaccgaacg
     4861 cgcaccccgc gacgcctggg ccccgaacct gcccgaccct gcaacggtca accgtccgtc
     4921 cagccaggcg ccgatcgacg gcggtccatc caatcccatc ggcggcaccg ccgccggtcg
     4981 tgatccccag tagagacgcc ttgcaccatt tcgaactgaa ggacggcgcc ctccacgccg
     5041 agggcgtcgc actcgaggcg ctggccgaca ccgtcggcac gccggtctat gtctattcga
     5101 gcgccaccct gacgcggcac tacacggtac tgcgcgaggc ggcggatgcg caccgcaatg
     5161 cgttgggcga cgcccttatc gcctatgcgg ttaaggccaa ctccaacctg tcggtgctgg
     5221 cgacgctggc gcggctgggc tgcggcgccg acacggtctc ggaaggcgaa atccgccggg
     5281 cgctggcggc gggcgttccg gcctcgaaga tcatcttttc cggcgttggc aagaccgaca
     5341 gcgaactggc cttcgccatc acggcgggcg tgcgccagat caatgtcgag agcccggccg
     5401 aactggaccg gctcattccg atagccgaac ggctcggtgc ggtgcctgag atcgccatcc
     5461 gggtgaaccc gaagatcggc gccggtggcc acgccaagat caccacgggc ggcgaaggcg
     5521 acaagttcgg cattcccgca gacgaggcga tggagctcta tgtccgcgcc gccgcctcgc
     5581 cgcatgtcac gcccgtcggt ctcgcctgcc acatcggcag ccagatcacc gacctgacgc
     5641 cgctggaggc cgccttccgc ctgctgcgat cgctgactga acagctgcgc gcccgtggcc
     5701 agacggtgac gcggctcgac ctcggcggtg gtttgggcgt gccctacgtg ggcggcgctc
     5761 ccactgcctc cccggcggac tacgtcgcga tggcggcgcg agtgctcgac ggcctcgacg
     5821 tcgaggccgc gtttgaaccg ggacgcctgc tggccgccaa cgcaggcgtg ctcctgtcgc
     5881 gcgtgatcca gatcaccgaa cgcgctgacg gccgccgttt cctcgtcctc gatgcggcca
     5941 tgaacgacct gatccgccct gcgatgtacg acagcttcca cgaggtccag ccggtccgcc
     6001 cgcgtgcgtt cggcggccac aacgccccca ccgcctacga catcgtcggc cccatctgcg
     6061 agactggcga caccttcgcc cgtggccgcg acctgccccc gctacaggcc ggggacctcg
     6121 tcgtcttcac cggcgcagga gcctacggcg cggtgatgtc gagcgaatac aacacccgcc
     6181 cgctagttcc cgaggttctg gtcgacggcg aacgctacgc ggtcatccgc ccgcgtccga
     6241 cctacgaaga gatgttggcg cgcgagccga tggcggaatg gcttcaagag taggcagtag
     6301 ggaataggca ggtaggcaac agtcgttcag cttgctggac acgccagtcc tgaaaacctg
     6361 cctactgcct aatccagcgc acaaatgtcc ggcaggccgc gcagggcgcc tgcgttgtcg
     6421 agaccgtagc cgacgaggaa gcggtcgggc gcctgccagc cgaagaaatc gggctcggcg
     6481 gcgcttggga gggggaacgg tttgcgggcg aagacgcagg tcagaacctc cgacgctccg
     6541 gcttccttgg caatccgtac ggcttccgcc agcgacaggc cggtgtcgaa gacgtcgtcg
     6601 aggatcagca ccttgcgacc cttcaggtcg cgctggaacg gggcatagac atcgatccgc
     6661 ccccggctgg acttttcgtc gccgtaggac gcgagccaca gagcgtcgaa gcggacatga
     6721 cgcccgaccc gcgacagggc tcgggtcagg tcggcggcga accacaaccc gcccgtcagc
     6781 aagactgcgg cgacggtgtc gtcgtcgata cgaggcgcaa tcttttcggc gagctcggcc
     6841 acgacccggg tgatatcagc ctcggacagc aggatttcgc gcgtggagga catctggggg
     6901 gaggagtcag ccatgatgcg aagcggatac cgcttcgcca tgcccgatgt cgataggccg
     6961 ggcgtcgacc ggagtgagcg cgggcggggc gttgacctcg tggttcaccg ccgggcgcag
     7021 gccgctggag gccg
//
LOCUS       DASVTM010000414         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_358564,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000414 DASVTM010000000
VERSION     DASVTM010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358564"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..619
                     /locus_tag="VF633_11500"
     CDS             62..619
                     /locus_tag="VF633_11500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8471728.1"
                     /translation="MLNLELQHRVKNNLAVVQGLARQTAKTSPDPSDFNRAFHGRLMA
                     LSQANDILLTGKWEECRLPELVEAALKPFLKEGIITTAGPPCRVPARPCVPLVLALHE
                     LGTNAVKYGALSDPCGQVRLTWRIENGTAVFDWEETGGPHVEAPKRKGLGSRLLSKQP
                     GLADVRVEFRPEGLTCRITIEGASA"
     gene            860..2203
                     /locus_tag="VF633_11505"
     CDS             860..2203
                     /locus_tag="VF633_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8471729.1"
                     /translation="MSDVASPTLLHDLVAAALKAGADAAEAVTSERRALSVGVRNGEL
                     EEVEREESRDLGLRVFVGQRQASVSASDLSEATQARLIERVVAMARLAPEDPYASLAP
                     QDRLNMGPHRELDLYDGSERSAAELEAAAAETEAVALAVEGVVRSEGGHASTSSSEWR
                     LVTSHGFDGRYQGSAFSLGVGVIAEKDGGMERGGESRTTRFLADLPDAWTIGAEAGRK
                     AVARTGPRKIASITAPVIFENRVATQILSPLLGAISGASIARGTSFLKHDLGKMLLPA
                     GMSLIDDPFRPRGLGSTPFDDEGVTVEKRRIVDDGILTTWLLNSGTAKQLGLRSTGHA
                     SRGLAGPPGVSTHNLHLTQGLRDKAGLMADAGTGLLVTSMFGPSLNGNTGDWSAGVSG
                     FWFEDGELAYPVSEVTVAGNLKALWARMVAGSDLEFRGAFNSPSLLFDAVAIAGK"
BASE COUNT          383 a          767 c          727 g          361 t
ORIGIN      
        1 ggcttcgcaa tcactacggg cgtcggtccg ggaactccat gcaaacgcca atcgtgaagc
       61 catgctgaac ctcgaacttc agcaccgggt gaagaacaac ctggcggtcg tacaaggtct
      121 tgcacgccag accgcaaaaa ccagtccgga cccctccgac ttcaacaggg cgttccatgg
      181 acggctcatg gcgctgtctc aggccaacga catccttctg acgggcaaat gggaagaatg
      241 ccgtcttcca gaactggtcg aggccgcgtt gaagcccttt ctgaaggagg gcatcatcac
      301 gaccgcagga ccgccatgtc gggtgcctgc ccgcccctgc gtcccgttgg tgctagccct
      361 gcatgagctg ggcacaaatg ccgtcaaata tggcgctctg tccgatccgt gcggccaggt
      421 ccgcctcaca tggcgcattg agaacgggac ggcggtcttt gattgggaag aaaccggagg
      481 cccgcatgtg gaagcgccaa aacgcaaagg cctcggctcg cgcctgttga gtaaacagcc
      541 cggcctggcc gatgtcaggg tggagttcag acccgagggc ctgacctgcc ggatcacgat
      601 cgaaggcgct agtgcctgaa cgacgaacag accgcttact gcagcgccgg cattgccggt
      661 tcccaagcca gaaatcgaca ctacaggctg acctctcgaa agccgaaagt gcaacgtccg
      721 caactggtcg aagtttgtgc ttggctgatc ggccgaacgc ggacatcgcc cggcgcacag
      781 gccttggtcc gtcgccggtc tgaccacccc atttgccgct cgccgttgcg gcatgcgccc
      841 cagtccgcca tatcggggca tgtccgatgt cgcctcgcct accctgttgc atgatcttgt
      901 cgccgccgcg ctgaaggccg gagccgacgc ggccgaggcc gtcacctccg aacgccgcgc
      961 gctttcggtt ggggttcgca acggggagct tgaagaagtc gagcgcgagg agtcgcgtga
     1021 cctgggcctg cgggtgttcg tcggccagcg tcaggcctcg gtgtccgctt cggatctgtc
     1081 ggaggcgacg caagcccggc tgatcgaacg ggtcgtggcc atggcgcggc tggcgcccga
     1141 ggatccctat gcgtcgctgg ccccgcagga ccggctcaac atgggaccgc accgcgaact
     1201 ggatctgtac gacggcagcg aacgatccgc cgccgaactg gaagccgccg ccgccgagac
     1261 cgaggccgtg gcgctcgccg tcgaaggcgt agtccggtcc gagggcggac acgcctcgac
     1321 ctcgtccagc gagtggcggc tggtcacctc gcacggtttc gacggccgct atcagggcag
     1381 cgccttctcg cttggcgtcg gcgtcatcgc cgagaaggac ggcggcatgg agcgcggggg
     1441 cgagagccgc accacccgct tcctcgccga cctgcccgac gcctggacca tcggcgcgga
     1501 ggccggtcgc aaggccgtgg cccgcaccgg cccgcgcaag atcgcctcga tcaccgcacc
     1561 ggtaatcttc gagaaccgcg tcgcgaccca gatcctgtcg cccctgctcg gggcaatctc
     1621 cggcgcctcg atcgcgcgcg gcacctcgtt cctcaagcat gacctcggca agatgctgtt
     1681 gcccgccgga atgagcctga tcgacgaccc gttccgcccg cgcggtctcg gctccacgcc
     1741 attcgatgac gagggcgtca cggtcgagaa gcgccgcatc gtcgacgacg gcatactgac
     1801 cacctggctg ttgaactcgg gcacggccaa gcagcttggc ctgcgttcga ccggccacgc
     1861 ctcgcgcggg ctggcgggcc ctcccggcgt ctcgacccac aacctgcacc tgacgcaagg
     1921 cctgcgcgac aaggcgggac tgatggcgga cgccgggaca ggcctgctgg tcacgtcgat
     1981 gttcggcccc tcgctgaacg gcaacaccgg cgactggtcc gccggggtgt cgggcttctg
     2041 gttcgaagac ggcgagctgg cctatccggt gtccgaagtc accgtcgccg gtaacctcaa
     2101 ggccctatgg gcgcggatgg tggcggggtc ggacctcgag ttccggggcg ccttcaacag
     2161 cccgtcgctg ctgttcgacg cggtggccat cgccggcaaa tgacggatta cgccaccgat
     2221 ctcgacctga tccgcgcc
//
LOCUS       DASVTM010000415         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_521097,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000415 DASVTM010000000
VERSION     DASVTM010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521097"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VF633_11510"
     CDS             <1..535
                     /locus_tag="VF633_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6655 family protein"
                     /protein_id="HEX8471730.1"
                     /translation="RTATEQLLLARAADRAVEHLTLPLPLGSRIFVDGTFFNVDNPSY
                     AISSIRNAVSEAGYALADSRDKADAVFELRAGALSLEQMRRVVGLPPLAVPVNETLNV
                     VSIPELSIYSRRDRVGIAEFSGFLYDAQTGAPLGAIAPMIGTFRIRSHKVMMVIAWGQ
                     QSLSPGEREPGDSWREF"
     gene            complement(532..960)
                     /locus_tag="VF633_11515"
     CDS             complement(532..960)
                     /locus_tag="VF633_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471731.1"
                     /translation="MKPVLIVLLGVPAAALAGGLALGAEVAIYMLIEAIRAGDSIALL
                     WLSGIMVGLYAALFFVAGLAVMGLPVLWGLGLAKRSGPVSAALAGAVGATVAVTGMMV
                     FGGVTDSALGFAAFLILPGALAGWMLWRIAFRPAAVTTLA"
     gene            complement(957..1121)
                     /locus_tag="VF633_11520"
     CDS             complement(957..1121)
                     /locus_tag="VF633_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471732.1"
                     /translation="MLRQWWWITLAIILASVVAVFGGVSSPEVLLLCPAIMLIPGAVA
                     GSMVRKVAYA"
     gene            1303..>2466
                     /locus_tag="VF633_11525"
     CDS             1303..>2466
                     /locus_tag="VF633_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX8471733.1"
                     /translation="MSDIAADLTEAVLETERPLTQDGPAIRLYLIDASAYIFRAYHAL
                     PPLTRKSDGLPVGAVQGYCNMLWKLLQDMKGADGPTHLAAIFDHSDKTFRNEMYDQYK
                     ANRSAPPEDLIPQFPLVREATAAFGVHCVELAGYEADDLIATYACKARDAGGEAVIVS
                     SDKDLMQLIGRGVVMYDPMKDKRLAEPEVFEKFGVTPDKMVDLQALIGDSVDNVPGAP
                     GIGPKTAAQLLDEYGDLDTLLERAGEIKQPKRRETLINFRDQILLSRELVRLTCDAPA
                     PETIDDFAVRDPDPATLSAFLESMEFRSLLRRVGDGKTADHSGQAFTAPQNRPAPKIP
                     QLGAPVITPRYAPKAVNTEVEAHVFDHDAYHCIQDLEALDAFIARATEAGVIGF"
BASE COUNT          461 a          878 c          748 g          379 t
ORIGIN      
        1 ccgtactgcg accgagcagt tgctgctcgc gcgggctgcc gaccgggccg tcgagcacct
       61 gaccctgccc ctgccgctcg gaagccgcat cttcgtcgac ggcacgttct tcaacgtcga
      121 caatcccagc tacgccatca gctccatccg caacgctgtg tccgaggccg gttatgccct
      181 cgcagacagt cgcgacaagg ccgacgccgt cttcgaactg cgggccgggg cgctgtcact
      241 cgaacagatg cgccgggtcg tcggactgcc gccgctggcg gtacccgtca acgaaaccct
      301 gaacgtggtc tcgatccccg aactgtcgat ctacagccgc cgtgaccggg tcggcatcgc
      361 ggagttttcg ggctttcttt acgacgccca gaccggggct ccgctgggag ccattgcgcc
      421 gatgatcggc accttccgca ttcgcagcca caaggtgatg atggtcatcg cctgggggca
      481 gcaatcgctg tcacccggcg aacgcgagcc cggcgatagc tggcgcgagt tctaggcgag
      541 cgtagtaact gcggcgggtc ggaacgcgat ccgccacagc atccacccgg cgagtgcccc
      601 cggcaggatc aggaaagcag cgaagcccag agcgctgtcc gtaactccgc caaacaccat
      661 cattccagtg acggcgaccg tggccccgac ggctccagcg agagcggcgc ttacggggcc
      721 gctccgcttc gctagcccga gcccccacaa gaccggcagt cccatgaccg ccagaccggc
      781 gacgaagaac agggcggcat agaggcccac catgatgccg gagagccaca gaagggcgat
      841 gctatccccc gctctgatcg cttcgatcag catgtagatc gcgacctcgg cgcccagagc
      901 cagaccgccg gccaaagccg cggccgggac gccgagcaac acgatgagaa ccggcttcat
      961 gcgtaagcga ccttgcgcac catcgacccg gcgaccgcgc cgggaatgag catgatcgcc
     1021 ggacaaagaa gcaggacttc cggactgctc accccgccaa acaccgcaac cacagaggcc
     1081 aggatgatcg ccagtgttat ccaccaccat tgtctcagca cgatagcccc ctgcgcgcgg
     1141 ccttgagaaa cgtagaacca tcgcccatcc ggtgtcggag cttatcgcct gggatgcaag
     1201 cgcgcccatg ttttcgaccg gcgccttgtg aaggccgccc atgtcgatcc cacagcacac
     1261 cgaccggtga cgccgccgcc tcagccccct atgttgctca ccatgtccga catcgccgcc
     1321 gacctgaccg aagccgtcct cgagaccgag cgccccctga cccaggacgg ccccgccatc
     1381 cgtctctatc tgatcgacgc ctcggcctac atcttccgcg cctaccacgc ccttccgccc
     1441 ctcacccgta agtcggacgg gctgccggtc ggggcggtgc aggggtactg caacatgctg
     1501 tggaagttgc tgcaggacat gaagggggcg gatgggccga cgcatctggc ggcgattttt
     1561 gatcattcgg acaagacgtt ccggaacgag atgtacgacc agtacaaggc caaccggagc
     1621 gcgccgccgg aggatctgat tccgcagttt ccgctggtca gggaggcgac ggcggcgttt
     1681 ggggtgcatt gcgtcgagct ggcggggtat gaggcggatg atctgatcgc gacctatgcc
     1741 tgcaaggcgc gggatgcggg gggcgaggcc gtcatcgtct cctcagacaa ggatctgatg
     1801 cagctgatcg ggcgcggcgt cgtcatgtac gatccgatga aggacaaacg cctcgccgag
     1861 cccgaggtgt tcgagaagtt cggcgtcacc ccggacaaga tggtcgatct gcaggccctg
     1921 atcggcgaca gcgtcgacaa cgtgcccggc gcgcccggca tcggacccaa gactgcggcc
     1981 caactattgg acgaatacgg cgacctcgac accctgctgg agcgcgcggg cgagatcaaa
     2041 cagcccaaac gccgcgagac cctgatcaat ttccgcgacc agatcctgct gtcccgcgag
     2101 ctcgtgcggc tgacctgcga cgcccccgcg cccgagacca tcgacgactt cgccgtgcgc
     2161 gaccccgacc ccgcgaccct gtcggccttc ctcgagtcga tggagttccg gtccttgctt
     2221 cgccgcgtcg gcgacggcaa gacggcggac cactcgggtc aggccttcac cgccccacaa
     2281 aaccgtcccg ctccgaaaat cccgcaactc ggcgcccccg tcatcacccc ccgctatgcc
     2341 cccaaggccg tcaacaccga ggtcgaggcg cacgtcttcg accacgacgc ctatcactgc
     2401 attcaggacc tcgaggcgct ggacgccttc atcgcgcggg cgaccgaggc cggtgtcatc
     2461 ggtttc
//
LOCUS       DASVTM010000416         4927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_41195, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000416 DASVTM010000000
VERSION     DASVTM010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41195"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VF633_11530"
     CDS             <1..1740
                     /locus_tag="VF633_11530"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX8471734.1"
                     /translation="MGLKGYRTGGTMHFVINNQIGFTTSPRNSRSTPYCSDVALMVQA
                     PIFHVNGDDPEAVVFAAKVATEFRQQFAKDVVIDVFCYRRFGHNEGDDPTFTQPLMYS
                     KIRAQPSTRELYSKRLVEEGVISQADIDAEVSRFEAWLDEQFEAGKTFKAEKADWLDG
                     QWKGVGLAEDDERRGKTEVPAEKLMDYGHRLTTIPNSIDMHKTLKRVIEARRESLNTG
                     NIDWATAESLAFASLLDEGFPVRLSGQDSVRGTFSQRHSGIIDQTTEERNVPLNNIRE
                     GQSNFEVIDSALSEEAVLGFEYGYSLSDPNTLVMWEGQFGDFVNGAQVVIDQFISSGE
                     RKWLRMSGLTMLLPHGYEGQGPEHSSARLERFLQQCAEDNMQVANCTTPANYFHILRR
                     QMQRPFRKPLILMTPKSLLRHKKAVSSIADMAEGSSFHRVLHDDAQTRPEVSGITIKA
                     DKDIRRVVLCSGKVYYDLLDAREKKVKAGQACDDVYLLRLEQFYPWPMKSISNELSRF
                     PDAELIWCQEEPKNMGGWTFVDPWLELTLDKLKVKTRRARYVGRPASASTAAGQMSRH
                     LKELQAFTDEAFA"
     gene            1847..2188
                     /locus_tag="VF633_11535"
                     /pseudo
     CDS             1847..2188
                     /locus_tag="VF633_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
     gene            2210..3415
                     /gene="odhB"
                     /locus_tag="VF633_11540"
     CDS             2210..3415
                     /gene="odhB"
                     /locus_tag="VF633_11540"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX8471735.1"
                     /translation="MPVPTMGESVAEGSMGKWLKKNGDTVKKDEMLVEIETDKVAVEV
                     SAPADGVLTITAEEGATVTPGQSIGSIAAGAGAAAAAPAAAPAAAATPAASANSGSAQ
                     FKPDATLSPSVQRIVTEGNLDASGIAPTGPKGNITKGDALAAAAPAPAAAAPAPVKAA
                     APAAPRVDQPREERVKMTRLRQTIARRLKESQNTAAQLTTFNEVDMTQVMALRTAYKD
                     AFEKRHGVKLGFMSFFTKAVINALHEIPAVNAEIDGTDIIYKNHYDIGVAVGTDKGLV
                     VPVLRDADSLSLAGIEKGIAELGKAARDGALTMDQMQGGTFTITNGGTYGSLMSTPIL
                     NAPQSGILGMHNIVQRPVAVNGQVVIRPMMYLAVSYDHRIVDGKEAVTFLVRVKEHLE
                     DPQRFLLDL"
     gene            3507..3920
                     /locus_tag="VF633_11545"
     CDS             3507..3920
                     /locus_tag="VF633_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471736.1"
                     /translation="MRHSSRMDWTARGIGGCYAVAGLIALRYVLINWWLDRRFAMVLR
                     PSPTELTADVLLATGAVLLTVSGLALSVLHPWAIPAFLACWAIQAVYLLWAQRWFRPR
                     CVASARGRRGTLHAFAIYSLATAFVLSLPLVGVLD"
     gene            complement(3917..4660)
                     /locus_tag="VF633_11550"
     CDS             complement(3917..4660)
                     /locus_tag="VF633_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8471737.1"
                     /translation="MKSSAPYRTGQRLPLEPATAGGETLQLRLSVNPRARRISIRIDA
                     RANEVVAVAPSERRLSEAVAFARSKAGWIAERMEARVEGQPLIPGQIILLHGLQTRLE
                     ATGTNGAARLTADADGPVIRSGGEGEAFARRVENLLKREARTQLLARTEVHLQALRQK
                     PVKVSVVDTRSRWGSCSPHNRSIRYSWRMVMAPPAVLDYLAAHEVAHLVHGDHSPAYW
                     SVVHGLVGDHRPFRAWLKANGQALHAVGR"
     gene            complement(4614..>4927)
                     /locus_tag="VF633_11555"
     CDS             complement(4614..>4927)
                     /locus_tag="VF633_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX8471738.1"
                     /translation="LLPRGLLEHDGKRVDLTSINDIGLMTVEGENDDISGVGQTQAAH
                     ILCTNIPDDRRVLYVQPDVGHYGVFNGRRFREEIYPRTRDFIATNEKLCAVPDRSATA
                     A"
BASE COUNT          898 a         1684 c         1525 g          820 t
ORIGIN      
        1 atggggctga agggttaccg caccggcggg acgatgcatt ttgtcatcaa caaccagatc
       61 ggcttcacga cctcgccgcg caattcgcgc tcgacgccct attgctcgga cgtggcgctg
      121 atggtccagg cgccgatctt ccacgtcaac ggcgacgatc cggaagccgt ggtgttcgcc
      181 gccaaggtcg ccaccgaatt ccgccagcag ttcgccaagg acgtggtgat cgacgtcttc
      241 tgctaccgcc gcttcggcca caacgaaggc gacgatccca ccttcaccca gccgctgatg
      301 tattccaaga tccgcgccca gccctcgacg cgcgagctct attcgaagcg tctggtcgag
      361 gaaggcgtga tttcgcaggc cgacatcgac gccgaggtct cgcgcttcga agcctggctc
      421 gacgaacagt tcgaggccgg caagaccttc aaggccgaga aggccgactg gcttgacggt
      481 caatggaagg gcgtaggcct cgccgaggac gacgagcgtc gcggcaagac cgaggtcccg
      541 gctgaaaagc tgatggacta tggccaccgc ctgacgacca ttccgaactc catcgacatg
      601 cacaagacgc tcaagcgcgt catcgaagcg cgtcgcgaga gcctgaacac tggcaacatc
      661 gattgggcca cagccgagag cctggcgttc gcgtccctgc tggacgaggg cttcccggtc
      721 cgtctgtccg gtcaggattc ggtgcgcggc accttctcgc agcgtcactc gggcatcatc
      781 gaccagacca ccgaggagcg taacgttccg ctcaacaaca tccgcgaagg ccagtccaac
      841 ttcgaggtca tcgactcggc cctgtccgag gaagccgtgc tcgggttcga atacggatac
      901 tcgctgtcgg accccaacac cctagtcatg tgggaaggcc agttcggcga cttcgtgaac
      961 ggcgcccagg tcgtgatcga ccagttcatc tcgtccggcg aacgcaaatg gctgcggatg
     1021 agcggcctga cgatgctgtt gccgcacggc tatgaaggtc agggtccgga acactcctcg
     1081 gctcgcctcg agcggttcct gcagcagtgc gccgaagaca atatgcaggt cgccaactgc
     1141 accaccccgg ccaactattt ccacatcctg cgtcgccaga tgcagcggcc gttccgcaag
     1201 ccgctgatcc tgatgacgcc caagtcgctg ctgcgtcaca agaaggcggt ctcgagcatc
     1261 gccgacatgg ccgagggctc gtccttccac cgcgtgctgc atgacgacgc ccagacgcgt
     1321 cccgaagttt cgggcatcac catcaaggcc gacaaggaca tccgccgcgt cgtgttgtgt
     1381 tcgggcaagg tctattacga cctgctcgac gcccgcgaga agaaggtgaa ggccggtcag
     1441 gcctgcgacg acgtctacct cctgcgcctc gaacagttct atccgtggcc gatgaagtcg
     1501 atctcgaacg aactgtcgcg cttccccgac gccgaactga tctggtgtca ggaagagccc
     1561 aagaacatgg gcggctggac cttcgtcgat ccttggctgg aactgacgct cgacaagctg
     1621 aaggtgaaga ccaggcgcgc ccgttatgtt ggtcgtccgg cctcggcctc gaccgctgca
     1681 ggccagatga gccgccacct taaagaactg caggccttca cggacgaggc tttcgcctga
     1741 taagcgagac cttcgttcgt cggcccacct gacgagacct gcccgactaa ccgttacaag
     1801 cccgccctag aacgcctgaa gcctttaccg aaagcctgga cccgacatgg ccgacatcct
     1861 gacccccacc cttggtgaat ccgtttcgga agccacgatc gcgaaatgga ccaggaaggt
     1921 cggtgacgcg gtgaagaagg acgaagttct ggtcgagctg gaaacggaca aggtctcgct
     1981 ggaagtcgtg gctccggccg atggcgtcct gtcggacatc aaggccgcgg atggcgatac
     2041 ggtcgttccg ggcacggttc tgggcgtcgt caccgaaggc gccgccgctg cgaaacccgc
     2101 cgctgctcct gccgccaagg ccgagaaggc tgcaccagct ccagcagccg ccgcacctga
     2161 agccgctccc gccgccgcac cggcctcggc tggcggcgcc tcagtcgcga tcccggtccc
     2221 gaccatgggc gagagcgtcg ccgagggttc gatgggcaag tggctcaaga aaaacggcga
     2281 caccgtcaag aaagacgaaa tgctggtcga gatcgagacc gacaaggtcg cggtcgaagt
     2341 ctccgctccc gctgacggcg tcctgaccat caccgccgaa gaaggcgcga ccgtcacccc
     2401 gggccagagc atcggctcga tcgctgccgg cgcaggcgcc gctgctgcag cccctgccgc
     2461 cgctccggct gcggctgcga ctcccgcggc ctcggccaac tccggttcgg cccagttcaa
     2521 gccggatgcc accctctccc cctcggtcca gcgcatcgtc accgagggca acctcgacgc
     2581 ctccggcatc gctccgaccg gaccgaaggg caacatcacc aagggtgacg cgctcgccgc
     2641 cgccgcgccc gccccggccg ctgcagcccc ggctccagtg aaagccgccg cccctgccgc
     2701 accgcgcgtc gaccagcctc gcgaagaacg cgtcaagatg acccgtctgc gccagaccat
     2761 cgcgcgccgc ctcaaggaat cgcagaacac cgccgcccag ctgaccacct tcaacgaggt
     2821 ggacatgacg caagtcatgg ccctgcgtac cgcctacaag gacgcgttcg agaagcgcca
     2881 cggcgtcaag ctcggcttca tgagcttctt caccaaggcg gtgatcaacg ccctgcacga
     2941 gatcccggcc gtcaacgccg agatcgacgg caccgacatc atctacaaga accactacga
     3001 catcggcgtc gccgtcggca ccgacaaggg actggtggtc ccggtcctgc gtgacgccga
     3061 cagcctgtcg ctggccggca tcgagaaggg catcgctgaa ctcggcaagg cggcccgcga
     3121 cggcgctctg accatggacc agatgcaggg cggcaccttc accatcacca acggcggcac
     3181 ttatgggtcg ctgatgtcga cgccgatcct caacgcgccg caatccggca ttctcgggat
     3241 gcacaacatc gtgcaacgcc cggtggcggt gaacgggcag gtggtgatcc gccccatgat
     3301 gtacctggcc gtcagctacg atcaccgcat cgtcgacggc aaggaagcgg tcaccttcct
     3361 cgtccgcgtc aaggaacatc tggaagaccc acagcgcttc ctgctcgacc tctagagaca
     3421 ctagaccgcg cgccggttgg accggcgcgc tgggcgtcac caacgccggc aggccggggc
     3481 aaacgcctat ttaccctgcg ttgcgaatgc gccattcttc gcgcatggat tggacggcgc
     3541 gaggtatcgg gggatgctac gcggtcgccg ggcttatcgc cctgcgatac gtgctgatca
     3601 attggtggct ggatcgccgg ttcgcgatgg tcctccgccc ctccccgacc gagttgactg
     3661 ccgatgtgct gctggcgaca ggggcggttc ttctgaccgt ctccgggctg gcgctgtcgg
     3721 tgctccatcc atgggccatt cccgccttcc tcgcctgctg ggcgattcag gctgtctatc
     3781 tgttatgggc tcaacgctgg tttcgccccc gatgcgtggc ctcggcccgg gggcggcgag
     3841 ggaccctcca cgcctttgcc atctacagcc tcgcaacggc gttcgtgctc agcctgcctc
     3901 tggtgggcgt gctggattaa cggcccacgg cgtgcagcgc ctgtccgtta gccttcaacc
     3961 aggcgcggaa cggccggtga tctccgacca gcccatgcac caccgaccag taggccgggc
     4021 tgtggtcgcc atgaaccaga tgggccacct cgtgcgcggc cagataatcg agcaccgccg
     4081 gcggggccat gaccatgcgc catgaatagc ggatcgagcg attgtgcggg ctgcacgagc
     4141 cccagcgcga gcgggtgtcc accaccgaaa ccttgacggg cttttgcctt agagcctgaa
     4201 gatggacctc ggtgcgggcc agcaactgtg tccgggcctc gcgcttgagc aggttctcga
     4261 cccggcgcgc aaaggcttcg ccctcgccgc ccgaacggat caccggtccg tcggcatccg
     4321 ccgtcagtcg tgccgcgccg ttcgtgccgg tcgcttccag acgggtctga agaccatgca
     4381 gcaggatgat ctgaccaggg atcaacggtt gaccctcgac cctcgcctcc atccgttcag
     4441 caatccagcc ggccttggag cgcgcaaacg ccacggcctc ggacaggcgg cgctcgctgg
     4501 gagccacggc cacgacctcg ttcgcccgcg cgtcgatgcg gatcgagatc cggcgggcgc
     4561 gggggttcac cgatagacgg agctggagcg tctctccacc cgcagtcgcg ggttcaagcg
     4621 gcagtcgctg accggtccgg tacggcgcag agcttttcat tggtcgcgat gaagtcccgg
     4681 gtgcgcggat agatttcctc gcggaagcgc cggccattga agacgccgta gtggcccacg
     4741 tccggctgga catagagcac gcgacggtcg tcgggaatgt tggtgcaaag tatatgcgcg
     4801 gcctgggtct ggccgacgcc ggaaatgtcg tcgttctcgc cctcgaccgt catcaggccg
     4861 atgtcgttga tggatgtcag atccacgcgc ttgccgtcgt gttccagcag gccgcgtggc
     4921 agcaggt
//
LOCUS       DASVTM010000417         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_521296,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000417 DASVTM010000000
VERSION     DASVTM010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521296"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF633_11560"
     CDS             <1..211
                     /locus_tag="VF633_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471739.1"
                     /translation="FSPELWTYEHLWKMVGAYSAVLSAFSGNFLRFPAPPWSALWPTI
                     LFQFVSIVWIGVLLVRKRRAVTLAA"
     gene            223..1068
                     /locus_tag="VF633_11565"
     CDS             223..1068
                     /locus_tag="VF633_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8471740.1"
                     /translation="MPRISFAAAIGAFAAYSYGATAVSAAWFLRRDYGFGLVPSIVWA
                     ALLFSPWVVVGLMVWAVLNRISAPARAITVLATLVAPVVPATALASTLIDSELRKADW
                     TWSVVVERSVDRLPVAILLYTAVAACGLAAVWWRRADRQRREIGSLSAALKALRSAQA
                     AEVWAPSAATALIVSMGRRRVPVHPAEIEWVSSAGNYAVVHWSDGGQDQEGLLRETLQ
                     ALATRLEPARFARGHRSTLINLAHVAELKPLADGAWRATMDSGAELVVSRTHRDVFLA
                     RLGRR"
     gene            1183..>2313
                     /locus_tag="VF633_11570"
     CDS             1183..>2313
                     /locus_tag="VF633_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8471741.1"
                     /translation="MRLRSVSLAALLAVIAPLPALIAAPVLAQTSAVPTALASRQAAE
                     LRAAPVAELVSEVNIPWKRFVLDNGLTVIVHEDRKAPVVALSVWYNVGSKDEPAGSTG
                     FAHLFEHLMFGGSENAPGSYLGRMRSLGPSNLNGTTWFDRTNYFETVPTPALEQALYL
                     ESDRMGYLLGEIGQPVLDLQRGVVQNEKRQGDNEPYGLFEYAQLEALFPEGHPYRHST
                     IGSMADLDAASLETVRNWFRENYGPNNAIVVLSGDIDEATARPLMQKYFGGIPRGPVN
                     TPAEADVPTLAAPVIATMHDRVATTRLTRSWAVPGLLSDDTVPLSVAASVLGGLASSR
                     LDNQLVRGEQTAVSVSSAVSEFHRVGMFDISVDVKPGQDAAEV"
BASE COUNT          349 a          778 c          746 g          440 t
ORIGIN      
        1 gttcagtcca gagctgtgga cctatgaaca tctatggaag atggtgggcg cctacagtgc
       61 ggtcctgtcc gccttctccg gcaattttct acgctttccc gcgccgccat ggagcgccct
      121 ctggccgacg attcttttcc agttcgtgag catcgtctgg atcggcgtcc tgctggttcg
      181 caaacgtcgc gccgtcacat tggccgcgtg agcgttcggg ctttgccgcg gatctcattt
      241 gcggctgcga ttggtgcatt cgccgcctac agctacgggg ccacggcggt gtcagcggcg
      301 tggttcctca gacgggacta cggcttcggc ctggtgccct ctatcgtctg ggcggccctg
      361 ctgttttcgc cgtgggtggt cgtcggattg atggtctggg ccgtcctgaa ccggatcagt
      421 gcccccgccc gggcaatcac cgtcctggcg accctcgtcg cgccggtcgt tcctgcaaca
      481 gcgctggcct ccaccttgat cgatagcgaa ttgcgcaaag cagactggac ctggagcgtg
      541 gtcgtggagc gctcggtgga ccgtctaccg gtcgccatct tgctctacac cgccgtcgca
      601 gcatgcgggc tggcggcggt ctggtggcgc cgcgctgatc gacagagacg cgagatcggt
      661 tccctttcag ccgcgttgaa ggccctgcga tcggcgcagg ctgcagaggt ttgggcgccg
      721 agcgcggcca cagcactgat cgtctcgatg ggccgtcgtc gcgtcccggt ccatccggcc
      781 gagatcgagt gggtttcgtc cgccggcaac tacgccgtcg tccactggtc cgacggtggc
      841 caggatcagg aaggcctgtt gcgcgagacg ctgcaggcgc tggcgacccg acttgagcct
      901 gcgcggtttg cgcgcggcca ccgctcgacc ctgatcaacc tcgcccatgt cgccgaactc
      961 aagccgctcg ccgacggcgc gtggcgcgcg acgatggaca gcggcgccga gctcgtggtc
     1021 agtcgaaccc atcgcgacgt ctttctggcg cgtctcggca gacgctgatc agcgcaccag
     1081 gcgtcggtct gctgattgtg catgcgtcac agtctgtgtg attgcgcaac gcgaaacgtg
     1141 gggtaaggtc cggccgcttc aaggaaaagg actgttcctc acatgcgtct ccgctccgtc
     1201 tcgctcgccg ccctcctggc cgtgattgct cccctccctg ccctcatcgc cgcgcccgtt
     1261 ctggcccaga cgtccgccgt accgacggcc ttagcatcgc gccaggccgc ggagctgcgc
     1321 gcggctccgg tcgccgagct ggtgtccgaa gtgaacatcc cctggaagcg cttcgtactc
     1381 gataacggtc tgaccgtgat tgtgcatgag gaccgcaagg cgccggtggt cgcgctgtcg
     1441 gtctggtaca acgtcggctc caaggacgag ccggccggat cgacaggctt cgcacacctg
     1501 ttcgaacacc tgatgttcgg tggatcggag aacgcacccg gcagctacct cggtcgcatg
     1561 cgcagcctgg gtccgagcaa tctgaacggc accacctggt tcgatcgcac caactatttc
     1621 gagaccgtgc cgacaccggc gctggagcag gcgctgtatc tggaaagcga ccggatgggc
     1681 tatctgctcg gcgagatcgg ccagccggtg ctcgatttgc agcgcggcgt ggtccagaac
     1741 gagaagcgtc agggcgacaa cgagccctac ggcctgttcg aatacgccca gctcgaagcc
     1801 ctcttccccg aaggtcaccc gtatcgccat tcgaccatcg gctcgatggc cgatctggac
     1861 gccgcgagtc tcgagacggt gcggaactgg ttccgcgaaa actacggccc gaacaacgcc
     1921 atcgtcgtcc tgtctggtga tatcgacgag gcgaccgccc ggccgctgat gcagaaatac
     1981 ttcggcggta tcccgcgcgg cccggtgaac accccggccg aggcggatgt gccgaccctt
     2041 gccgccccgg tcatcgcgac catgcatgac cgcgtcgcca ccacccgact gacccgcagc
     2101 tgggcggtgc ccggcctgct gagcgacgac accgtaccgt tgtcggtcgc ggcctccgtg
     2161 ctgggcggcc tcgcctcctc gcggctcgac aaccagctgg tacgcggcga gcagaccgct
     2221 gtttcggtgt catcggcggt atcggagttc caccgcgtcg ggatgttcga catctcggtc
     2281 gatgtgaagc cgggtcagga cgccgctgaa gtc
//
LOCUS       DASVTM010000418         5990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_678219,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000418 DASVTM010000000
VERSION     DASVTM010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678219"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /gene="recQ"
                     /locus_tag="VF633_11575"
     CDS             complement(<1..1794)
                     /gene="recQ"
                     /locus_tag="VF633_11575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX8471742.1"
                     /translation="MPAESATLSKPAAAGMDDAREILHRVWGHTDFRGLQADVVGEIL
                     AGRDVLAVLPTGGGKSVCYQIPAILRPGLGLVVSPLIALMTDQVEALKQQGVAAARLD
                     SGVSMDDRSAIWRAARAGELDLLYVSPEGLAQPHLIDRLHELPLSLIAIDEAHCVSQW
                     GHDFRPDYRTLGRLAELFPGVPRIAVTATADARTRDDIVRSLRLDAAEVFVDSFARPN
                     LQLSAVRKENASRARTDAAVIDLVRQRRGKSGVVYCGSRDGCDRVAQALRDAGTNAIA
                     YHAGMGTDDRDKRLARFLAEDGAVMVATIAFGMGVDKADVRFVIHADPPGSLEAYWQE
                     IGRAGRDGEPAEGITLYGPSDIAWALKRLDGRPMAEEVKQVQVSKARRLFAMLDGAVC
                     RPQAVQRYFGETDAGVCGVCDVCTDPPALYDATVQAQKALAAVQRLGGRFGRNRVIDH
                     LLGKTREVQPWESDLSTWGIGKDVSPNGWRDIVDHLLFEGLLLEDANDGKPLVGLGDP
                     EAVRAVYRGERAIEVRRIPAGSATGRSEPPRRARNDRNAAVEGLDADVRARFETLRAW
                     RRDRASEQHVPPYVIFQDKTLLEIAQVEPGSM"
     gene            complement(1843..2997)
                     /locus_tag="VF633_11580"
     CDS             complement(1843..2997)
                     /locus_tag="VF633_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX8471743.1"
                     /translation="MTTDTRPITTRAAVLRSMGAAHPYADSRPLSIETVTLDPPGPGE
                     VLIAIKAAGLCHSDLSVINGDRPRPLPMALGHEAAGVVERLGEGVTDLAVGDHVVMVF
                     MPSCGHCNPCAEGRPALCEPGAVANGKGELLAGGKRIHHDAETLNHHLGCSAFADRAV
                     VSRRSLVKIDPDLPLDEAALFGCAVLTGVGAVVNTAGIRAGQSAVVVGLGGVGLASVL
                     GALASGASPVIAVDLSEDKLALARTLGAVQTVNASDADAVEQVRALTNGGADFVFEMA
                     GSVRALDAAWRMTRRGGTTVTAGLPPPDAALAVNVVSLVGEERTLKGSYIGTCVPSRD
                     IPRYVALYRQGRLPVDKLMSGKIPLEQINEGFDRLHAGQVVRQIVAFEGA"
     gene            3170..3604
                     /locus_tag="VF633_11585"
     CDS             3170..3604
                     /locus_tag="VF633_11585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8471744.1"
                     /translation="MAIREEGPLADRATYKAFHTVSTRWADNDQYGHINNAKFYEFVD
                     SAVNAHLIIADALSESIGLVVDSGCRYTSSLAFPDVIEVGIKVDHIGNSSVKYGFAVF
                     RRGVSVPAAIGHFVHVYVDAETRRPKTLPPKLRAVVDNLKAG"
     gene            complement(3665..3865)
                     /locus_tag="VF633_11590"
     CDS             complement(3665..3865)
                     /locus_tag="VF633_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471745.1"
                     /translation="MNGPDYAAEAAGYEDDDLDEADIVEWYEARPVTVSTAMATGSIV
                     AAFALGVLTAVGALTLIGYLDD"
     gene            4085..4858
                     /locus_tag="VF633_11595"
     CDS             4085..4858
                     /locus_tag="VF633_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8471746.1"
                     /translation="MAGHSKFKNIMHRKGRADAVRSKLFSKLSRDITVAAKAGMPDVA
                     LNPRLRLAVNNAKAESLPKDVIQRAINKAAGGDVDTLEEVRYEGRGPGGVGIIVEALT
                     DNRNRANSNIGTAFKKNGGALGEMNSVAFMWDKVGQIIYKAEAGTEDAVMEAAIEAGA
                     QDVESDLVKPDIYEDAPGHIIWTAFEDLNEVAEAMSKILGDAKSTAIVWKPQTEVPVT
                     GEHVGTLMRLLDALDAEDDVSSVYSNEEISDEDMAKYAG"
     gene            5092..>5990
                     /locus_tag="VF633_11600"
     CDS             5092..>5990
                     /locus_tag="VF633_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471747.1"
                     /translation="MMLSRIDRVLSRNGKGDRAPKPSAPVEAAMGPDAAGVIGERFET
                     IQDSLDQLSDMAQHFGTFESLLVQLRDPLEAEFRSRRDNHVELVNLRAAHAENAGRLD
                     IASAETRRLAIALAETEARADDLAARNGEQGAALQDARVETDRLRSDLSLSASRVEAL
                     EIAERASGQRIRELEQDQDSLRTQLKQAESRRTESESARTQIQRDHALVLEENTVLRR
                     RTDEVSAEVASLARAAAASEGQLATERARAASEQSETARNTRALENQTETARAEIAAL
                     SSRLDTATARANGLEILNTEQASR"
BASE COUNT         1077 a         1934 c         2037 g          942 t
ORIGIN      
        1 catcgagccg ggctcgacct gcgcaatctc cagcagggtc ttgtcctgaa agatgacgta
       61 gggcgggacg tgctgttcgg aggcgcggtc gcgacgccag gcgcgcaggg tctcgaagcg
      121 ggcgcggacg tcggcgtcca gaccttcgac tgcggcgttg cggtcgttcc tcgcacggcg
      181 cggcggctcg gagcgaccgg tcgccgagcc agccggaatg cgacggacct cgatcgcgcg
      241 ctcgccacga tagacggcgc ggaccgcctc gggatcaccc agtccgacca gcggcttgcc
      301 gtcgttggcg tcctcgagca gcaggccttc gaacagcaga tgatcgacga tatcgcgcca
      361 gccgttcggg gaaacatcct tgccgatgcc ccaggtggag aggtcgctct cccagggctg
      421 gacctccctg gtcttgccca gcaggtgatc gatcacgcgg ttacgaccga agcgtccgcc
      481 gaggcgctgg accgcagcca gagctttctg ggcctgaacc gtggcgtcgt agagggcggg
      541 tggatcggtg cagacatcgc agacaccaca gacccctgca tcggtctcgc caaaatagcg
      601 ttgcacggcc tgaggtcggc aaacggcccc atcgagcatg gcgaacaggc ggcgggcctt
      661 ggagacctga acctgtttga cttcttcggc catcggtcgg ccgtccagcc gcttcagggc
      721 ccaggcgata tccgacgggc cgtagagggt gatgccctcg gcgggttcgc cgtcgcgtcc
      781 tgcccggccg atttcctgcc agtaggcctc cagcgaaccg ggcggatcgg cgtggatgac
      841 gaagcgaacg tcggccttat cgacgcccat gccgaaggcg atggtggcga ccatgaccgc
      901 gccgtcctcg gcgaggaagc gggcgaggcg cttgtcgcgg tcatccgtgc ccatgcctgc
      961 gtgataggcg atagcgttgg tccccgcgtc gcgcagggcc tgggccacgc ggtcgcagcc
     1021 gtcacgtgag ccgcaataga cgacgcccga cttgccacga cgctggcgca ccagatcgat
     1081 gacggcggcg tcggtgcgag cgcgggacgc attttccttg cgcaccgccg agagctgcag
     1141 gttcgggcgc gcgaagctgt cgacaaagac ctcggccgcg tcgagccgca gcgaacggac
     1201 gatatcgtcg cgcgtccggg cgtcggcggt ggcggtgacg gcaatgcgcg gcacgccggg
     1261 gaacagctcg gccagcctgc cgagcgtgcg gtagtcgggg cggaaatcat ggccccattg
     1321 cgagacgcag tgggcctcgt cgatggcgat caggctgagc gggagttcgt gcaggcggtc
     1381 gatcaggtgc ggctgtgcca gaccttcggg cgagacgtag agcaggtcca gttcaccggc
     1441 gcgcgcggcg cgccagatgg ccgaccggtc atccatcgac acaccggagt cgagacgcgc
     1501 ggcggccacg ccctgctgct tcaacgcctc gacctgatcg gtcatcaggg cgatcagggg
     1561 ggagacgacg aggccgagac cggggcgcag gatggcgggg atctgatagc agaccgactt
     1621 gccgccgccg gtaggcagca cggccagtac gtcgcgcccg gcgaggattt cgccgacgac
     1681 atcggcctga agaccgcgaa aatcggtatg gccccagacg cgatgcagga tctcgcgtgc
     1741 gtcatccatg cccgctgctg caggtttgga aagggtggcg gattcggcgg gcatcgcgcg
     1801 cttgtgcccg atctgttccg ggctgtcacg aagcgatgag gttcaggcgc cttcgaacgc
     1861 cacgatctgg cgcacgacct gtccggcgtg cagtcggtcg aagccttcgt tgatctgttc
     1921 cagcggaatc ttgccgctca tcagcttgtc gacgggcagt cggccctgcc gatagagggc
     1981 gacgtagcgg gggatgtcgc ggctcgggac gcaggtgccg atgtatgagc ccttcagcgt
     2041 ccgctcctcg ccgaccagcg agacgacgtt gaccgcaagc gcggcgtcgg gcggcgggag
     2101 gcctgcggtg acggtggtcc caccgcgccg ggtcatgcgc caggcggcgt cgagcgcgcg
     2161 gaccgagcct gccatctcaa acacgaaatc cgctccacca ttcgtcaacg cgcgcacctg
     2221 ttccaccgcg tcggcgtccg aggcgttgac ggtctggact gccccgagcg tgcgggccag
     2281 cgccagcttg tcctcggaca gatcgaccgc gatgaccggc gacgcgcccg acgcgagcgc
     2341 gcccagcacg ctcgccaatc cgaccccgcc gagcccgact acgacggcgc tctgacccgc
     2401 gcggatgcct gcggtgttca cgaccgctcc gacgcctgtc aggaccgcgc agccgaacag
     2461 cgcggcctcg tccagcggca gatcagggtc gatcttcacc agcgaccggc gcgaaaccac
     2521 ggcgcggtca gcgaaggcgg agcaaccgag gtggtggttc aaggtctctg cgtcgtggtg
     2581 gatgcgcttg ccgcccgcca gcagttcgcc cttgccgttg gcgacggcgc ccggttcgca
     2641 cagggcgggg cggccctcgg cgcaggggtt gcagtggccg cacgacggca tgaagaccat
     2701 gaccacatgg tcgccgacag cgaggtcggt gacgccttcg cccaaccgtt ccaccacgcc
     2761 cgccgcctcg tgccccagcg ccatcggcag ggggcgcggc ctgtctccgt tgatgacgct
     2821 gagatcggag tggcacaggc ccgccgcctt gatcgcgatc aggacctcgc cgggaccggg
     2881 cgggtcgagc gtcacggtct cgatcgaaag cggccggctg tcggcatagg ggtgggctgc
     2941 gcccatggat cgcaggacag cggcgcgggt ggtgatgggg cgtgtgtcag tggtcatctg
     3001 gcggcctcgt gatcgatggc tcatcgatag gcgcttgacg cgacgtcggc caagcccgag
     3061 cgtcacagat gaacggcgac gctgtggaag ccgaacgtac tcaactgcgc tcaagcagcc
     3121 gacgaccgcg tcggaggcgg tagcgccact aaaaagggac aaggaaacga tggcgatccg
     3181 cgaagaaggg ccgttggccg atcgggccac ttacaaggct tttcacacgg tctcgacccg
     3241 ctgggcggac aacgaccagt acggccatat caacaacgcc aaattctacg agttcgtcga
     3301 ctcggcagtg aacgcccacc tgatcatcgc cgacgccctg tcggaatcca ttggtctggt
     3361 ggtcgatagc ggctgtcgtt acacctcatc gctggctttt cccgacgtaa tcgaggtcgg
     3421 gatcaaggtc gatcacatcg gcaattcgag cgtcaaatac ggcttcgcgg tgttccgccg
     3481 cggcgtcagc gttcccgccg ccataggcca tttcgtgcat gtctacgtcg atgcggagac
     3541 gcgccgtccg aagaccctgc cgcccaagct acgggccgtg gtggacaatc tgaaagcggg
     3601 ctgagttcgc gtcgaagacc ggggcctgcc gaccggcacg gtcttcgacg gctgttgtac
     3661 gtcgctagtc gtccaggtag ccgatcagcg tgagggcgcc gacggcggtc agaacgccga
     3721 gcgcgaaggc cgcaacgatc gatccggtcg ccatggcggt cgagaccgtg acaggacggg
     3781 cttcatacca ctcgacgatg tcggcctcgt cgagatcgtc gtcttcgtag cctgcagctt
     3841 cggcggcgta atcaggtccg ttcatcggaa atctcttctt gcttgtgcgc aggcaccggc
     3901 tcgggggagc ctggatgctc tagcgtcgtg tcacgactaa atccgcctca ggcttggcgg
     3961 ttccgcgccc gtcatgcaac tgcggtgtcg tgacagccgc gcgggcatcg gctaaacgcg
     4021 ccggtcagaa ccaaacagac ctcgctcaag cagcgagcgt tgcgcccagg accaagagac
     4081 ccagatggcc ggccattcca aattcaagaa catcatgcac cgcaagggtc gcgccgacgc
     4141 ggtgcgctcc aaactgttct ccaaactgtc gcgcgacatc accgtggccg ccaaggcggg
     4201 catgcccgac gtggcgctga acccccgtct tcgtctggcc gtcaacaacg ccaaggccga
     4261 aagcctgccc aaggacgtga tccagcgggc gatcaacaag gccgccggcg gcgacgtcga
     4321 tacgttggag gaggtccgct acgagggccg gggcccgggt ggtgtcggca tcatcgtcga
     4381 ggccctgacg gacaaccgca atcgcgccaa ctccaacatc ggcacggcgt tcaagaagaa
     4441 cggcggcgca ctgggcgaga tgaactcggt cgccttcatg tgggataagg tcggtcagat
     4501 catctacaag gccgaggccg gcaccgaaga cgccgtcatg gaggccgcta tcgaggccgg
     4561 cgcacaggac gtcgagagcg atctggtcaa gccggacatc tatgaggacg ctccgggcca
     4621 catcatctgg accgccttcg aggacctgaa cgaggtggcc gaggccatgt caaagatcct
     4681 cggcgacgcc aaatccacgg ccatcgtctg gaagccccag accgaagtgc cagtcacggg
     4741 ggagcatgtc ggcaccctga tgcgactgct ggacgcgctg gacgccgagg atgacgtctc
     4801 gtcggtctat tcgaacgagg aaatctccga cgaagacatg gcgaagtacg ccggttgacg
     4861 gcgccgggac ggtagtgagc tccggtgctg gcagtaccgg ttttgatcga tctttcggca
     4921 ccgagcggat tggtccgtcc tgagggttat cccccgccga tcagggttac ctgtatcgac
     4981 tcaactgact gtgctcgtta acatttcgtt gaaggggacg ccgggggtct gattcgacac
     5041 gttgccgcag taggcagctt cccgcgtgcg tccgccaatc ggagtgcgac catgatgctg
     5101 tccaggatcg accgggtttt gtccaggaat ggcaagggtg accgggctcc caagccttcg
     5161 gcgccagttg aggcagcaat gggaccggac gccgccggcg tgatcggcga acggtttgaa
     5221 acgattcagg acagtctcga tcaactgtcg gatatggccc agcatttcgg cacgttcgaa
     5281 agcctgctag tacaactgcg cgacccgttg gaagcggagt tccgctcgcg tcgtgacaat
     5341 cacgtcgaac tcgtcaacct tcgcgccgcc catgccgaaa acgctggacg acttgacata
     5401 gccagcgccg agacgcgccg actggctatt gcattggccg agaccgaggc ccgcgccgac
     5461 gacctggctg cgcgcaacgg cgagcaaggc gcggctttgc aggatgctcg tgtcgaaacc
     5521 gaccggttgc gcagcgacct ttcgctcagc gcaagccgcg tcgaggcgct cgagatcgcc
     5581 gaacgcgctt cggggcagcg gattcgggag cttgaacagg atcaggactc gctgcgcacc
     5641 cagctcaaac aggctgagag ccgacggacg gagtccgaga gcgcccgcac ccagatccag
     5701 cgcgaccatg ctctggtgct ggaagaaaac accgtcctgc gacgccgcac ggatgaggtc
     5761 tcggccgagg tcgcgtcgct ggctcgcgca gcggccgcca gcgaggggca gctcgccact
     5821 gagcgggctc gcgcggcttc cgagcaaagc gagacggccc ggaatacgcg ggcgctggaa
     5881 aaccagaccg agactgcacg cgcggagatc gccgccttgt cgtcgcgcct ggacacagcc
     5941 accgcacgcg ccaacggtct ggaaatcctc aataccgaac aggcatcgcg
//
LOCUS       DASVTM010000419         5261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_41335, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000419 DASVTM010000000
VERSION     DASVTM010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41335"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VF633_11605"
     CDS             complement(<1..440)
                     /locus_tag="VF633_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEX8471748.1"
                     /translation="MVKQIALSIRRLALKIASYGLMHLVVAILVAFAITRDWRLALAV
                     GVVEPLFQTVAYTIHDRVWHRIEQRGVLSLPEETVEAVGARLDVMNAEEQTRSHGHAG
                     HGHAGHGHAGHSHALPRSFGKVALKTVTYGLMHFTVAVSVAYALT"
     gene            577..2037
                     /gene="guaB"
                     /locus_tag="VF633_11610"
     CDS             577..2037
                     /gene="guaB"
                     /locus_tag="VF633_11610"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX8471749.1"
                     /translation="MEIREGLTFDDVLLEPGPSETMPAEADVSTRLTREIRLNIPLTS
                     SAMDTVTESRLAIAMAQAGGLGVLHRNMTVEEQADQVREVKRYESGMVINPVTITPET
                     TLGEIRAIVARKKISGFPVIDPVSGKLVGILTNRDMRFDTDPNRKAGELMTRGDLITV
                     REGAGRDEARALLRDRKIERVIVVDEDYRATGLITMKDIEKAQAHPHAAKDDQGRLLV
                     GAASTVGDAGFERAMALADAGCDVVVIDTAHGHSAQVSRVVERIKRENNRLQIIAGNI
                     ATYDAAKALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIIDAVRAAKDSGTPVI
                     ADGGIKYSGDLAKAIAAGASVAMMGSMFAGTDESPGEVFLYQGRSYKSYRGMGSVGAM
                     GAGSADRYFQKEVEDTQKLVPEGIEGQTPYKGPISPVLHQMVGGLRAAMGYVGAPTIA
                     EFQERARFVRITGAGLRESHVHDVMITREAPNYRQG"
     gene            2049..2441
                     /locus_tag="VF633_11615"
     CDS             2049..2441
                     /locus_tag="VF633_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8471750.1"
                     /translation="MTPELTYLALTLILAVVQIFLPATGRTLQFGSKWNAGPRDEAMP
                     PVSPVTGRLERAQSNLYETLPLFIGAVLIAHVAGEDGFLTYWGATLYFWSRVVYVPLY
                     AFGVTGVRSLAFLASLVGLLMIIAALFI"
     gene            2450..3862
                     /locus_tag="VF633_11620"
     CDS             2450..3862
                     /locus_tag="VF633_11620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX8471751.1"
                     /translation="MTPAARLASAASVLDSIAQGRLPAETVLKTWASENRYAGSKDRR
                     AVADRVYLVLRARGRLVAAMGGREDGRALVIGSLSLLDGLSLEQIEALHSGQGYGPKP
                     LSKQERSRLTVPTDEQPEPVAAGLPEFVLEDFKSTFGDNWAVEVEALMVPRAPIDLRV
                     NSAKATVAEVEAELRAAGLKPQRTPWSAWGLRLNAEPAPDIQAMAVFKQGRVEIQDEG
                     SQITCWLANVAPGMTVVDYCAGGGGKTLGLAMQMAAVSTGRVVESEEAAKVWTPTGWV
                     DAPVAKKAAAPQQTEGQLVAYDVVQKRLDNIKPRLQRAGASAELHQLGPNGGGVEDMI
                     QQADVVFVDAPCTGSGAWRRRPEDAWRLKPEDVDRMHTLQMGIVARAAKLVKPGGRLV
                     YVTCSMLRGENESTLDAFEEDHPSFMPVAIADAVNTANFTSAGRGKLASLAAGGHRLR
                     LSPATSGTDGFFVAVYERQA"
     gene            3859..4530
                     /locus_tag="VF633_11625"
     CDS             3859..4530
                     /locus_tag="VF633_11625"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262514.1"
                     /note="Enables the cleavage of the glycosidic bond in both
                     5'-methylthioadenosine and S-adenosylhomocysteine; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX8471752.1"
                     /translation="MSGGDFEFVKFGPITALCVMAAEAEYGPALRSRIKPLITGVGPV
                     EAAIATTAALAELAHADGLPDLVISLGSAGSQTLDHGRIYWVDEVAYRDMDATVLGFA
                     PGVTPFLGEPASLTLASGPQGQPYARLATGGSVVSGESYDAIDADMVDMETYAVVRAA
                     ARFGLPVIGLRGISDGKADLTVLEDWTGTLAVIDEGLAEALDLLANELTRLTADLPDA
                     KQEPS"
     gene            4527..>5261
                     /gene="guaA"
                     /locus_tag="VF633_11630"
     CDS             4527..>5261
                     /gene="guaA"
                     /locus_tag="VF633_11630"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX8471753.1"
                     /translation="MTTPSHTDPKALDPKLNHQKVLIVDFGSQVTQLIARRLREANVY
                     CEIHPYTKAAAALAAMSPAAIILSGGPESVHEEGSPRAPQAVFEAGVPVLGICYGEMT
                     MCEQLGGRVEGGLHREFGRAGITVEKTSPLLADLAVVGETESVWMSHGDKIVAIPEGF
                     DVVATSPGSPYAVIADETRRFYGVQFHPEVMHTPRGATILKNFTHGIAGLTGDWTMAA
                     YRDEKIAQIRAQVGSAKVICGLSGGVD"
BASE COUNT          903 a         1748 c         1746 g          864 t
ORIGIN      
        1 gtcagggcgt aggcgacact gacggcgacg gtgaaatgca tcaggccgta ggtgaccgtc
       61 ttcagagcta ccttgccgaa agagcggggc agggcgtggc tgtgtccggc atgcccgtgt
      121 ccggcatgcc cgtgtccggc atgaccgtgt gaccgcgtct gctcttccgc attcatgacg
      181 tccagtcgcg cgccgaccgc ctcaacggtc tcctctggca gactcaggac gccgcgttgt
      241 tcgatacggt gccagacccg atcgtggatc gtataggcca ccgtctggaa caagggttcg
      301 accacgccaa ccgcaagcgc cagccgccag tcgcgcgtga tggcgaaggc caccagtatc
      361 gccacgacga ggtgcatcag cccatagctg gcgatcttca gcgcgaggcg gcggatggag
      421 agggcgatct gcttgaccat gaaatcttaa atcaggccgg tgtgcataag ttgcaagggt
      481 caatgaaact ctgtgacgaa tgatgatctg ccagtgcgcg aagcttgact tcgcaatcgc
      541 gaaagaggat aggcggcccg caaaacggag actcccatgg agattcgcga aggccttacc
      601 ttcgacgatg ttttgcttga acccggcccg tccgagacca tgcctgcgga agcagatgtc
      661 tcgacgcgac tgacacgcga aatacgattg aacatcccgc tgacctcgtc ggcgatggac
      721 actgtgaccg aaagccgcct ggcgatcgcc atggctcagg ccggtggtct gggcgtgctt
      781 caccgcaaca tgacggtcga agagcaggcc gatcaggtcc gcgaggtcaa acgctacgaa
      841 agcggcatgg tcatcaatcc ggtgaccatc acgccggaga cgacgctcgg cgaaattcgc
      901 gccatcgtcg cccgcaagaa gatcagcggc tttccggtca tcgatccggt ttcgggcaag
      961 ctggtcggca tcctgaccaa ccgcgacatg cgtttcgaca ccgatccgaa ccgcaaggcg
     1021 ggcgagttga tgacgcgcgg cgacctgatc accgtgcgcg aaggcgccgg gcgggacgag
     1081 gcgagggcgc tgctgcggga tcgcaagatc gaacgcgtca tcgtcgtcga cgaggattat
     1141 cgcgccaccg gcctgatcac gatgaaggac atcgagaagg ctcaggcgca tccccacgcc
     1201 gccaaggacg atcaaggtcg tctgctggtg ggcgccgcct cgaccgtcgg cgacgccggg
     1261 ttcgagcggg ccatggctct ggccgacgca ggctgtgacg tggtggtcat cgataccgcc
     1321 cacggtcact cggcgcaggt ttcgcgggtg gtcgagcgca tcaagcgcga gaacaaccgc
     1381 ctccagatca tcgccggcaa catcgccacc tatgacgccg ccaaagccct gatcgacgcg
     1441 ggcgccgacg cggtgaaggt cgggatcggc ccgggcagca tctgcaccac ccgcatcgtc
     1501 gcgggcgtgg gcgtgccgca actgaccgcg atcatcgatg cggttcgcgc cgccaaggac
     1561 tcgggtacgc cggtcatcgc cgatggcggg atcaagtact cgggcgatct ggccaaggcc
     1621 atcgcggccg gggcctcagt cgccatgatg ggctcgatgt tcgcgggcac ggatgagtcg
     1681 cccggcgagg tgttcctgta ccagggccgt tcgtacaagt cgtaccgcgg catgggctcg
     1741 gtcggggcca tgggcgcagg ctctgcggac cgctacttcc agaaggaagt cgaggacacg
     1801 cagaagctgg tgcccgaagg catcgagggc cagacgccgt acaaggggcc gatcagtccg
     1861 gtgctgcacc agatggtcgg cggtcttcgc gccgccatgg gctatgtcgg cgccccgacg
     1921 atcgccgagt tccaggaacg cgcccgcttc gtgcgcatca ccggagccgg cctgcgtgag
     1981 agccacgtcc acgacgtgat gatcacccgc gaagcgccga actatcggca gggctgagga
     2041 tgacgaccat gacgcccgaa ctgacttatc tggccctgac gctgatcctg gcggtggtgc
     2101 agatcttcct gcctgcgacg ggtcggacgc ttcagttcgg gtcgaagtgg aatgcaggtc
     2161 cgcgcgatga ggccatgccg ccggtgtcac cggtgaccgg tcgcctcgaa cgggcccagt
     2221 ccaacctcta tgagaccctg ccgcttttca tcggcgccgt cctcatcgcc catgtcgcgg
     2281 gcgaggacgg atttctgacg tactggggcg ctacgctcta tttctggtcc cgcgttgtct
     2341 atgtgccgct gtacgccttt ggcgtaaccg gcgttcgcag cctggccttc ctcgcatcgc
     2401 tcgtcggctt gctgatgatc atcgccgccc tgttcatctg aaagcttaag tgaccccagc
     2461 cgcacgcctc gcctccgccg cctccgtcct cgacagcatc gctcagggcc gtctgcccgc
     2521 cgagactgtg ttgaagacct gggcctccga aaaccgttac gcggggtcca aggatcgccg
     2581 cgccgtcgcc gaccgcgtct atctcgtgct gcgggcgcgt ggacggctgg tcgcggccat
     2641 gggtggtcgt gaagacggtc gcgcgctggt catcggctcg ctgagcctgc tcgacggcct
     2701 gtcgctcgaa cagatcgaag cgctgcacag cggtcaaggc tacggtccca agcccttgtc
     2761 caagcaggag cgctcgcggc tcacagtccc gaccgacgaa cagccggagc cggtcgcggc
     2821 cggtctgcca gagttcgtgc tggaggactt caagtccacg ttcggcgaca actgggcggt
     2881 cgaggtcgag gccctgatgg tgcctcgcgc gccgatcgat ctgcgcgtca actccgccaa
     2941 ggcgacggtc gccgaggtcg aggccgaact ccgtgcagcc ggtctgaagc cacaacgcac
     3001 gccctggtcc gcctggggtc tgcgcctcaa cgccgagccc gcgccggaca tccaggccat
     3061 ggccgtgttc aagcagggcc gggtggaaat tcaggacgag ggcagccaga tcacctgctg
     3121 gctggccaat gtcgcgccgg gcatgacggt cgtcgattac tgcgccggtg gcgggggcaa
     3181 gaccctcggt ctggcgatgc agatggctgc cgtttcgacg ggccgcgtgg tcgagtccga
     3241 agaggccgcc aaggtctgga cgccgaccgg ctgggtcgat gcgcctgtcg ccaagaaagc
     3301 cgcagccccg cagcagaccg aaggccagct tgtcgcctat gacgtggtcc agaagcgtct
     3361 cgacaacatc aagccgcgcc tgcaacgcgc cggcgcctcg gccgaactgc atcagcttgg
     3421 ccccaacggc ggcggtgtcg aggacatgat ccagcaggcc gacgtcgtgt tcgtcgatgc
     3481 gccctgcacg ggctccggcg catggcgtcg tcgtcccgaa gacgcctggc ggctgaagcc
     3541 cgaagacgtc gaccggatgc acacgctgca gatgggcatc gtcgcccgcg cggccaagct
     3601 ggtgaagccg ggcggtcgtc tggtctatgt cacctgctcg atgctgcgcg gcgagaacga
     3661 gtccacgctc gacgccttcg aggaggatca cccgtccttc atgccggtgg cgatcgcgga
     3721 cgccgtcaac accgccaact tcacctcggc cggtcgtggc aagctggcca gcctcgctgc
     3781 gggcggtcac cgcctgcgac tgtcgcccgc gacctcaggc accgacggct tcttcgtcgc
     3841 cgtctatgag cggcaggcgt gagcgggggc gacttcgaat tcgtcaagtt cgggccgatc
     3901 accgccctgt gcgtgatggc cgccgaggct gaatacggcc cggcgctcag gtcccggatc
     3961 aagcctttga tcaccggcgt cggcccggtc gaggcggcga tcgccacgac tgccgcgctg
     4021 gcggaactgg cccatgcgga cggcctgccc gatctggtca tctcgctggg ttcggcagga
     4081 tcgcagacac tggaccatgg ccgcatctac tgggtggacg aggtcgccta tcgcgacatg
     4141 gacgcaaccg tgctcggctt cgcgccgggc gtgacgcctt tcctcggcga gcccgccagc
     4201 ctgaccttgg ccagcggtcc gcagggccag ccctatgccc gcctcgccac cggcggcagc
     4261 gtggtctcgg gcgagtccta tgacgccatc gacgccgaca tggtcgatat ggagacctat
     4321 gccgtggtgc gcgctgccgc ccgcttcggc ctgcccgtca tcgggctgcg cggcatttcg
     4381 gacggcaagg ctgacctgac cgtgctcgag gactggaccg gcacactcgc cgtgatcgac
     4441 gaaggtctgg ccgaagcgct ggacctgctg gccaacgaac tgacccggct gaccgccgac
     4501 cttcccgacg ccaaacagga gccgtcatga cgaccccttc ccacactgac ccgaaggctc
     4561 tggaccccaa gctcaaccac cagaaggttc tgatcgtcga tttcggcagt caggtgacgc
     4621 agttgatcgc gcgacgtctg cgcgaggcca acgtctattg cgagatccat ccctacacca
     4681 aggcggcggc ggcgctggcg gccatgagcc cggcggcgat catcctgtcg ggtgggcccg
     4741 agagcgtgca cgaggaaggc agcccccgcg cgccgcaagc cgtgttcgag gcgggcgtgc
     4801 cggtgctggg catctgctat ggcgagatga cgatgtgcga acagctcggc ggcagggtcg
     4861 aaggcggcct gcaccgtgag ttcggccgcg ccggcatcac cgtcgagaag acctcgcccc
     4921 tgctggccga tctggctgtg gtcggtgaga cagaaagcgt ctggatgagc cacggcgaca
     4981 agatcgtcgc catccccgaa ggcttcgacg tcgtggccac ctcgcccggc tcgccctacg
     5041 ccgtgatcgc cgacgagacg cgtcgcttct atggcgtaca gttccacccc gaggtgatgc
     5101 acaccccgcg cggcgcgacg atcctgaaga atttcaccca tggcatcgcg ggcctgaccg
     5161 gcgactggac catggccgcc tatcgcgacg agaagatcgc ccaaatccgc gcccaggtcg
     5221 gctcggccaa ggtcatctgc ggcctgtcgg gcggcgtcga c
//
LOCUS       DASVTM010000420         3787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_252838,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000420 DASVTM010000000
VERSION     DASVTM010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252838"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VF633_11635"
     CDS             complement(<1..1347)
                     /locus_tag="VF633_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX8471754.1"
                     /translation="MSLRFTASLAALGMAAAVLSAASTPARADEGMWTFDNFPIKTVN
                     DKYGTRIDQAWLDHVRGSAVRINGCSASLVSGEGLILTNWHCVVGCAQELSSAEHDYV
                     KNGFLTANREEEKECPGETAEILSDIVDVTDRVLGSGAGLEGAAFVQARGAETTKIED
                     ENCTDKQTQTCQVISFYRGGRYALYKFRKYEDVRLVFAPENQAAFFGGDPDNFNFPRY
                     ALDSGFLRIYEDGKPVATPTHLTWNANPPKEGDVTFVAGNPGSTSRLLTISQLERLRD
                     QVNPITLIQASELRGRIQEYANTSEEAKRISFDPLFGLENSFKVYYGQEAALTDPTFM
                     GKKREEEADLRAKVAADPAIAARIGDPWGDLEKVQTAARDLYLPYRQLEASAGGGSTL
                     YSYAKSIVRAAKERAKPVAERRAGYTDAALAQMSRRLAEVTPIDQGMEELYLTHWML"
     gene            1516..2688
                     /locus_tag="VF633_11640"
     CDS             1516..2688
                     /locus_tag="VF633_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8471755.1"
                     /translation="MSLALSTLLFEWRRYMAAVMALALSGLLVLAMTGVFIGIGKGFT
                     ATIERSSADIIIMQPGAKSLMGGPSGVPRRFIPLAYNHPEVVEVKPLDGSQGAFQNIK
                     DVDPTMSQADKAKQGAARQKYVQVSIVDTTPGAVTIPTDYSQELVDALRQPYTVAVDE
                     TALTTLDVKLGDKALYNGQTVTVAAVTRGYPNMMQPSIVMSRDTLRMLGQADTGARVG
                     PLMIKLRDPLKAGIVAAQLNAMGNGQWKAWTRQELADANAGAMFEEGILVIIIGGCVV
                     LGIIIGVAITWQTLRGAIMANIKEFASLRALGVGMGSLNRIVVELSFWVGCVGVVAAI
                     GLTWLVSLLAAANAVIIALPPMLLVVVGGGLILIAMVSGLLSLGILKNSQPADLLR"
     gene            2685..3449
                     /locus_tag="VF633_11645"
     CDS             2685..3449
                     /locus_tag="VF633_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8471756.1"
                     /translation="MTDFSQHTKVHGKHGDFAIEAKGLVKRFKSGKSFVEVLKGVDFD
                     ARHGDLTMVMGPSGSGKSTLIAALSGLLRPEAGRVDALDVDDMWKLSLGKIDKFRLAH
                     CGFIFQGFNLFPALTALQQVEIVLKFQGMTSGQAKKRATEALTEVGLAHRLHQRPAIL
                     SGGEKQRVAIARALAKDPKILFADEPTSALDGENGQVVIRLLRRAATEHGAAVICVTH
                     DPRLEAWADRVIKIEDGIIVSDERRIPNPDATLASH"
     gene            3470..>3787
                     /locus_tag="VF633_11650"
     CDS             3470..>3787
                     /locus_tag="VF633_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX8471757.1"
                     /translation="MPAFLKNKWLWIGILLIIVLGVGFTVFGNASKAKKAELEKAEAT
                     KVVSPYAAIANGKVDIEGGIIQIAARRGGIVREVLVQEGDTVVAGQILARQEDDEPRL
                     SLQT"
BASE COUNT          663 a         1193 c         1197 g          734 t
ORIGIN      
        1 cagcatccag tgggtcaggt aaagctcttc catcccttga tcgatcgggg tcacctcggc
       61 cagacggcgg ctcatctggg ccaaagcggc gtccgtatag ccggcgcggc gttcggcgac
      121 cggcttggcg cgttccttgg cggcgcggac gatcgacttg gcgtagctat agagcgtcga
      181 tccaccgccg gcgctggctt cgagctggcg atagggcagg tacaggtcgc gagcggccgt
      241 ctggaccttc tccagatcac cccaaggatc gccgatgcgg gcggcgatgg cgggatcggc
      301 ggcgaccttg gcccgcaggt cggcttcttc ctcgcgcttc ttgcccatga atgtcggatc
      361 ggtcagggcg gcttcctgac cgtagtaaac cttgaagctg ttctcgagac cgaacagcgg
      421 gtcgaacgag atgcgcttgg cctcttccga ggtgttggca tattcctgaa tccggccacg
      481 cagttccgag gcctgaatca gggtgatcgg gttcacctga tcgcgcagac gttccaactg
      541 cgagatcgtc agcaggcgcg aggtcgagcc ggggttgccg gcgacgaagg tcacgtcgcc
      601 ttccttgggt ggattggcgt tccacgtcag gtgggtcggc gtcgcgaccg gcttgccgtc
      661 ctcgtagatg cgcaggaaac cgctatcgag cgcatagcgc gggaagttga agttgtcggg
      721 atccccaccg aagaaggcgg cctgattttc aggcgcgaac accagccgga cgtcttcgta
      781 tttgcggaac ttatagaggg cgtagcgtcc cccgcgatag aagctgatca cctgacaggt
      841 ctgggtctgc ttatcggtgc agttctcgtc ctcgatcttc gtcgtctcgg cgccgcgggc
      901 ctgaacgaag gccgcgcctt cgaggcctgc gcccgagccg agcacgcggt cggtgacgtc
      961 gacgatgtcg gacaggatct cggctgtctc gccggggcat tccttctctt cttcgcggtt
     1021 ggcggtgagg aagccgttct tgacgtagtc gtgctcagcc gacgaaagct cctgtgcgca
     1081 gcctacgacg cagtgccagt tggtcaggat caggccttcg cccgagacca gcgacgccga
     1141 acagccgttg atgcggacag cggaaccgcg cacgtgatca agccacgcct gatcgatccg
     1201 ggtgccgtat ttgtcgttca cggtcttgat ggggaagttg tcgaacgtcc acatgccttc
     1261 atcggcacgg gcgggggtgg aggcggcgga caggacggcg gcggccatgc cgagcgctgc
     1321 aagtgaagca gtaaaacgaa gggacataca ggcgatctcc gaaaggactg gcggcttgct
     1381 aatccgaacg tcactcgccg tccatgccgt ccgtgttgct gcaacgccgc agtgccaaag
     1441 ccttaccccg atttaacttg gccttgatcg tcgacggcct cagataacgc caacactcgg
     1501 ggttgaaacg ctttcatgtc gctcgcactg tccacgctgc tgtttgagtg gcggcgctat
     1561 atggccgccg tcatggcgct cgccctgtcc ggccttctgg ttctggccat gaccggcgtc
     1621 tttatcggca tcggcaaggg cttcaccgcg accatcgagc gctccagcgc cgacatcatc
     1681 atcatgcaac cgggcgccaa gagccttatg ggcggtcctt cgggcgtgcc gcgccggttc
     1741 attcccctcg cctataacca tcccgaggtc gtcgaggtta agcctctcga cgggtctcaa
     1801 ggcgcctttc agaacatcaa ggacgtggat ccgacgatgt ctcaggcgga caaggccaaa
     1861 cagggcgccg cccgccagaa atacgttcag gtcagcatcg tcgacacgac accgggggct
     1921 gtgaccatcc ccaccgacta ttcgcaggag ctcgtggatg ccctgcgtca gccttacaca
     1981 gtcgctgtgg atgagacggc gctgaccacg cttgacgtca agctcggcga caaagccctg
     2041 tacaacggtc agaccgtgac cgtcgccgct gtgacccgcg gttatccaaa catgatgcag
     2101 ccttcgatcg tcatgtcgcg cgacaccttg cggatgctgg gtcaggccga caccggtgcg
     2161 cgggtggggc ctctgatgat caagcttcgc gatccgctga aggccggcat tgtggccgcc
     2221 caactcaacg ccatgggcaa cggtcagtgg aaggcctgga cgcgtcagga attggctgac
     2281 gccaacgccg gcgcgatgtt cgaggaaggc atcctcgtta tcatcatcgg tggctgcgtc
     2341 gtgcttggta tcatcatcgg cgtggccatc acctggcaga ccctgcgcgg ggctatcatg
     2401 gcaaacatca aggagttcgc ctcgctgcgc gccctcggtg tcggcatggg ttcgctcaac
     2461 cggatcgtcg tcgagctcag cttctgggtc ggctgcgtcg gtgtggtcgc ggccatcggc
     2521 ctgacatggc tcgtctcgct gctggcggca gccaatgctg tcattatcgc cctgccgccc
     2581 atgcttctgg tcgtcgtcgg cggcgggctg atcctcattg ccatggtgtc tggcctgctg
     2641 tcgctcggca tcctcaagaa cagccaaccg gcggacctgc tgcgatgacc gatttctcac
     2701 agcacacgaa ggtccacggc aagcatggcg acttcgccat cgaagccaag ggtctggtca
     2761 agcgcttcaa gtccggaaaa agtttcgtcg aggtgctcaa aggcgtcgat ttcgacgcgc
     2821 gccacggcga tctgaccatg gtgatgggac cgtccggttc gggcaagtcg acgctgattg
     2881 cggcgctgtc gggcctgttg cgtcccgagg ccggtcgcgt cgatgcgctt gacgtcgacg
     2941 acatgtggaa gctgtcgctc ggcaagatcg acaagttccg cctcgcccat tgcggcttca
     3001 ttttccaggg cttcaacctg ttcccggccc tgactgctct tcagcaggtc gagatcgtgc
     3061 tgaagtttca gggcatgacg tcgggtcagg cgaagaagcg cgccaccgag gccctgaccg
     3121 aagtcggtct ggcgcatcgc ctgcatcaac gtcccgccat cctgtcgggc ggtgagaagc
     3181 agcgggtcgc tatcgctcgc gccctggcca aggaccccaa gatcctgttc gccgatgaac
     3241 cgacgtccgc tctcgacggc gaaaacggtc aggtggtcat ccgcctgctg cgccgcgccg
     3301 ccaccgaaca cggcgccgcc gttatctgcg tgacccacga tccacgtctg gaggcctggg
     3361 ctgaccgggt catcaagatc gaggacggga tcatcgtctc ggacgaacgc cgcatcccca
     3421 atcccgacgc cacgctcgcg tctcactgac cgctctcagg accatcctca tgcccgcatt
     3481 tctgaaaaac aaatggctct ggatcggcat cctgctgatc attgtgctcg gcgtcggttt
     3541 cacggtgttc ggcaacgcct cgaaggccaa aaaggccgag ctggagaagg ctgaggcgac
     3601 caaggtggtc agcccctatg cagccatcgc caatggcaag gtcgatatcg agggcggcat
     3661 catccagatc gcggcccgtc gcggcggcat cgtccgtgaa gtccttgtcc aggaaggcga
     3721 cacggtcgtc gccggccaga tcctggcgcg ccaggaagac gacgagcccc gcctgtcgct
     3781 gcagacc
//
LOCUS       DASVTM010000421         5795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_995951,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000421 DASVTM010000000
VERSION     DASVTM010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995951"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1898)
                     /locus_tag="VF633_11655"
                     /pseudo
     CDS             complement(144..1898)
                     /locus_tag="VF633_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            2375..3427
                     /locus_tag="VF633_11660"
     CDS             2375..3427
                     /locus_tag="VF633_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEX8471758.1"
                     /translation="MNMLTTVGGKHEFLRQARNCRKMGSTFLASVLEAVDRQLPHAPI
                     STDMIMGWPGDPAAAAIALRFNGALHALARRGDPGYLAALYRGEHQDFDGAISAALSQ
                     EDRFIADWLRDPTQTNEVARAAAIMSALMSVRLERPMSFELLELGSSCGLNLNLARYA
                     YTLGGVSAGDPLSRVQITPQWRGADPRIAPVSIASAAGVDLHPLDASDADHRERLLSF
                     TWSDQPVRARRLEEALRIAVAHPPRVERGDASTWLAQRLAEPQEPGRCRAVVHTMFVQ
                     YLSDDERRDLATMIAAAGELATAERPLVWIGLEWTPDRSEVELRLTCWPRGETVVLAK
                     CHPYGDWLEWLAVSPA"
     gene            complement(3617..>5795)
                     /locus_tag="VF633_11665"
     CDS             complement(3617..>5795)
                     /locus_tag="VF633_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471759.1"
                     /translation="IRGLPLASSGSKYMQIQEDGLPVLEFGDFFNVASDIFIRADFNL
                     AAIEAIRGGSSSTFASNSPGGVINLLSRTGDVKGGAIQLTTGLDYETNRVDFDYGAPI
                     SDTLRFHVGGFYRSGEGPRDVGYNAFEGGQLKFNLTKTFTGGYVRLSGKYLDDRSPQY
                     LSGPVRITGANENPVFESYEGYDVRRDSMLSGSLPNLITLDGQNNPVQIPISQGMDAK
                     VRSIGVESQFTVHDWTITQRARYSQISGGTTRNLVSAVYAGNALPASLGGGTGTFTYA
                     TGPLAGQVINNLATVNGNGLVVASSLNRIDASNLDNFINDIRASRAFAVGDNELTVTA
                     GLYNSLQNLGSDWLYGNHIQVVGGDGASALINYTNAANVPQTQGGYLGFNRSGATAFY
                     RRKYDVEYNIVAPYGSLNYRFGRIAVGASVRYDSGDVSGQLYGADLGGGRIGIMPFDF
                     NGDGVISVAESRTAFTPLDRPAPVDYDYGYVSYSTGVNFRVSEPFAVFARYSRGARAG
                     ADKVLYSSKVSTIDGSMPDEADGYDIVEQLEGGFKYRRSGLTLNVTGFSADTEDTNVQ
                     AGAITTDRNYTAYGVEAEGGYQHGPFSLTGGVTWTQAEIVDDKLNATVNGMVPRRQPD
                     FLYQATPQFQSRQFTVGANLMGATSSYATDNDLMRVPGYLLVNAFLQYRPVARVQLML
                     NANNLFDEVAWIEITTPSVPANGVGLGRAANGRTISLSARMDF"
BASE COUNT         1005 a         1804 c         1830 g         1156 t
ORIGIN      
        1 ggacaacggc cagagcgggc tttggcagct cggcttcagt atcgggtcgg atgcccaccc
       61 atacctcaag ccacctttcg gaaggcacgg tcggcggcgc ctcgggagat ttcggcgatg
      121 tgcgagcccc cggcctccga ccctagcctg aaacgcgcga ccagcctggc cagttcgtcg
      181 gcctcggtgg cgaggttcct cgccgcggca gtggcttcct tgaccatggt cgcgttttgc
      241 ttcgtcatgc tgtccatact ggcaaccgcg ctgttcacct gcttcaactt gtcggcctgc
      301 gttccggtgc tatgggcgat ctcgctgatg agcgtgttgg tgtcgccgat ctttgaaccg
      361 atacgcgcca gcatcttgcc ggtgtcgccg acccggccca cgccgctcga gacctgctcg
      421 gacgacttgg taatcagtgc cttgatgtcc cttgccgcat cagcggaacg ctgggcgagc
      481 gcgcggacct cggtcgccac gacagcgaag cctcgcccgg cgtcaccggc gcgggcggcc
      541 tcgacgcccg cgttgagtgc gagaagattg gtctgaaagg cgatgccgtc gatcaggttg
      601 atgatctggc cgatttcctc ggaactggtc tggatggcgt ccatcgccat gacggcctcg
      661 tcgacgatgg cgctgccctc gctggcattg gtgttcgcga ccgtgattgc cgtgcgggca
      721 tccgcggcgc gttgagcggt ctgcccgacc atgctcgtga cctgctcggt cgcggtgctg
      781 gtttcctgaa gacgtgtggc ctgttgttcg gtccggcgtg agaggtcctc ggaggcggag
      841 tagatttcag cggcgccgga gtgaacgctt tgcgccgacc tggctacgct ggaaatgcta
      901 tgctcgagcc cttcgacggc gctgttgaac gatttgcgca gccgctcgta ttcaggcgcg
      961 aagggctggc tgatgacatg ggtcaggtcg ccggaagcga gcgcctcaag cgcggtgtcc
     1021 agagattcca cgacccgtcg ctgcgcctcg tcagcgccga ccttggcgag gtcggccagc
     1081 gctttcgccc ggtctgcctc cgccttggcc agatcagctt cagccttggc cagggcagcg
     1141 tcgcggaaga ccaggaccgc gcgcgccata tcgcccagtt cgtcagcacg ctcggcgtcc
     1201 acggtgatgt tgttatcgcc ttcggccagt tgcgccatcg cgacagtaag cgtcgtaatc
     1261 gggcgcgcga tcgtccggcc cagcatcacc gacagggtca tggcggtggc gatcagcgcg
     1321 ataccgccga ctatgagggc aacgacagcg gtgaggatgg cgtcttcctg gcgcgctccg
     1381 ttcttggcga tcagctccgc ctgtcccgtt cgaatatcgc gcaacggcag caccgcagcg
     1441 ctgaccaaca ccttgttgcc ggcgtcgcga acgatctgct gcgcctcatc gcgcccgcct
     1501 gcgcggacga cctcgatcat ccgatcgccc cagtccttgc gccaggcgag agtcgcggta
     1561 tgcgaatcct ccagactctt cagattggca gggtccttta acaggccctt catctcggtg
     1621 tagatggagt cgtactcgtc ccggccctca tagtaggact tgagatagct cgtgtcgccg
     1681 gtcacgagaa aacctcgaaa ctggctgttc tgcctcagga tcgcggtctc cagcgacagc
     1741 gcgttggcgt gaatggactg actcagattg ttgctgtccg tcgaggacct gatcatccag
     1801 atcgttccga agaacacagt catcatgatc gcggctgaaa cgcagatcag aacaaacgac
     1861 aggctcagtt ttcgggtaat tttcatcgca gcgatcatgt tcttgatttg caattttact
     1921 tacggatgac gcagttgaat ctcgatattt aagtgcagca atagtctcgg caaactgacg
     1981 cgtcagtgtt cgagattagg gccccaccca attccggttg actacatttg gttgaatgga
     2041 gggttaacgg gcgcaaaaat ggagcgcggc ggcacgaaac actggtccgg atctgtgctg
     2101 cagcctgttg aatttacgta tttatactta ctggcatcca cttgggccac tcagttcaag
     2161 atggaaccga catggtcccg accccctccc agccgccttc tacgcccgga actttatacg
     2221 aatttaacga tcgtggccta gcaactggag cagtaagaac aagccgcgat tacttgcttc
     2281 ggtaatgatg cttgatccga actattcggt gccggtacgt ccggattact ttgtaagcgc
     2341 gtcgggagta tatgcttatg ttgatcgcgg tttcatgaac atgctcacga ccgtcggcgg
     2401 caagcatgag tttttgcgcc aggctcgcaa ctgccggaag atggggtcca cgttccttgc
     2461 ttccgtgctc gaagccgtag atcgccagct tcctcatgcg ccgatcagca cggacatgat
     2521 catgggctgg ccaggggatc cggccgctgc ggccatcgcc ttgcgtttca acggggccct
     2581 tcacgcgctc gcccgacgtg gcgatccggg atatctcgca gcattgtacc gcggcgaaca
     2641 ccaggacttc gacggcgcca tctcggccgc cctgtcgcag gaagatcgct tcatcgccga
     2701 ctggcttcgt gacccgaccc agacgaacga agtcgcccga gccgctgcga tcatgtccgc
     2761 tctgatgtcg gtgcgtcttg agcgacccat gtcgttcgag ctgctcgaac tgggatccag
     2821 ctgcggcctg aacctcaatc ttgcacgata cgcttacact ctgggcggcg tctccgcggg
     2881 cgatcccctg tccagggtcc agatcacccc ccaatggcgc ggcgcggacc cacgcattgc
     2941 tccggtatcg atcgcttcgg cggcgggagt cgacctgcac ccgctggacg catcagacgc
     3001 agaccaccgc gaacgcctgc tgtcctttac ctggtccgac cagcccgtgc gggcgagacg
     3061 cctggaggag gcattgcgga tcgctgtcgc gcatccgcct cgcgtcgaac gtggcgacgc
     3121 atccacatgg ctagcgcagc gtctcgccga gcctcaggag ccaggacgct gccgcgctgt
     3181 cgtgcatacg atgttcgtgc agtatctcag cgacgacgaa cgtcgtgacc tcgcgacgat
     3241 gattgccgcg gcgggcgagc tggcgaccgc cgaacgtcca cttgtgtgga tcggccttga
     3301 atggacaccc gaccggagcg aggtggagct acgccttacc tgctggccgc gcggtgaaac
     3361 ggtggtgctc gcaaaatgtc atccttacgg ggactggctg gagtggctcg ccgttagccc
     3421 ggcctgacct tttccgggtc tgttgatacc cgccggacgt cggcgcgacc tcgaccgccc
     3481 tcctccagta ccgttatcca cggcacggcg cccttgtgtt ttcgccgggc tcgccttgga
     3541 gtcggttccg gcgaggaggc gatcccgcac ggcgaccgga ggacggccgt gcaggatccg
     3601 gctcaccttg cggtaatcag aagtccatgc gggccgagag agagatcgtg cgcccgttag
     3661 ccgcccgtcc caggccgaca ccattggctg ggaccgaggg tgtcgtgatc tcgatccagg
     3721 cgacttcgtc gaacaggttg ttggcgttga gcatcagctg cacccgcgcc acaggacggt
     3781 actgcaggaa ggcgttgacc agcagatacc ccggcacccg catcaggtcg ttgtccgtcg
     3841 catagctgct cgtggcgccc atcaggttgg cccccacggt gaactggcgg gactggaact
     3901 gcggtgtcgc ctgatacagg aagtccggtt ggcggcgcgg caccatgccg ttcaccgtcg
     3961 cgttcagctt gtcatcgacg atctcggcct gggtccaggt cacgcccccg gtcaggctga
     4021 acgggccgtg ctgatagccg ccctcggcct cgacgccata ggcggtatag ttccgatcgg
     4081 tcgtgatggc cccggcctgg acattggtgt cctcggtgtc ggccgagaag cccgtgacgt
     4141 tcagggtcag gccggacctg cgatacttga agccgccttc cagctgttcg acgatgtcgt
     4201 agccgtctgc ctcatcgggc atgctgccgt cgatcgtgct gaccttgctg gaatacagta
     4261 ccttgtcggc gccggcccgc gccccgcggc tgtaacgggc gaacaccgcg aacggctccg
     4321 aaacccggaa gttgacgccg gtcgaatagc tgacgtagcc gtagtcgtag tccaccggag
     4381 ccgggcgatc gagcggcgtg aaggcggtcc tcgattcagc gaccgagatg acgccatcgc
     4441 cgttgaagtc gaaaggcatg atgccgatgc ggccgccgcc gagatcggcg ccgtacagct
     4501 gcccactgac gtcgccgcta tcgtagcgga cgctggcccc gacggcgatc cggccgaagc
     4561 ggtagttgag cgacccgtag ggggccacga tattatactc gacatcgtat ttacgccggt
     4621 agaacgccgt ggcgcccgag cggttgaacc ccagatagcc gccctgggtc tgcggaacgt
     4681 tggctgcatt ggtgtagttg atcagcgccg acgcgccgtc gccccccacg acctggatgt
     4741 gattgccgta aagccagtcg ctgccgaggt tttgcaggga gttgtagagc cccgccgtca
     4801 ccgtcagctc gttatcgccc acggcgaagg cgcggctcgc gcggatgtca ttgatgaagt
     4861 tgtcgaggtt gctcgcgtcg atccggttca gcgagctggc gaccaccaag ccgttgccat
     4921 tcacggtggc caggttgttg atcacctggc ccgcgagcgg accggttgcg taggtgaagg
     4981 ttcccgtccc gccgcccagc gaggcgggca gagcattgcc cgcgtagacg gccgagacca
     5041 gattgcgcgt agtgccgccc gaaatctgcg aataacgggc gcgctgggta atcgtccagt
     5101 cgtgcaccgt gaattgggat tcaacaccga tcgagcggac cttggcgtcc atgccctggc
     5161 tgatcgggat ctgtaccgga ttgttttggc cgtcgagggt gatcagattg ggcaaggatc
     5221 cggacagcat ggaatcgcgg cggacgtcat agccctcgta gctttcgaac accgggttct
     5281 cgttggcgcc ggtgatccgg acggggccgg aaagatactg cggcgaacga tcgtcaaggt
     5341 acttgcccga gagacgcaca tatccgcccg tgaaggtctt ggtcaggttg aacttcagct
     5401 ggccgccctc gaaggcgttg taacccacgt cgcgggggcc ttcaccggac cggtagaagc
     5461 cccctacgtg aaagcgcaat gtgtcgctga ttggcgcgcc atagtcgaaa tcgaccctgt
     5521 tggtctcata gtcgagcccg gtggtcagct ggatcgcgcc acctttgaca tcaccggttc
     5581 tggacagaag attgatcacg ccgcccgggg aattcgatgc gaaggtcgac gacgatccgc
     5641 cgcggatcgc ctcaatggcc gcgaggttga agtcggcgcg gataaagatg tccgacgcga
     5701 cgttgaagaa gtcgccaaac tcgaggacgg gcaagccgtc ctcctgaatc tgcatatatt
     5761 tggatccgct ggacgccagg ggcaggccac ggatt
//
LOCUS       DASVTM010000422         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_734036,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000422 DASVTM010000000
VERSION     DASVTM010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734036"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VF633_11670"
     CDS             <1..1202
                     /locus_tag="VF633_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8471760.1"
                     /translation="RVLIIGAGHAGGSVAALLRQYGFEGEMVLAGRETVPPYQRPPLS
                     KAWLKGEADLDALLLRPASFYAEQKIELRTGVTATRVDPTSKTVTFTDGTVEAYDVLI
                     LATGSVARKLDIPGADRPDLIELRTLHEAERLKAALAPGKRLVVVGGGYVGLEAAASA
                     RTLGTEVVVIERMERVLQRVASETLSTFFTEHHRSHGVEIRTDAEVAGFDDGAVRLID
                     GELIRSDVILVGVGALACDALARTAGLACENGVVVDEMARTSDPSIYAIGDMTHRPIP
                     TMGGRVMRLESVPNALEQAKQAAAAIVGRVAPAPEVPWFWSDQYDIKLQIAGLPFGAD
                     RQVVRGDPGEGRFAVFHLVADRIVCVEAVNSPPEFMAGRLLIGRATAVDTAKLSDASV
                     SMKEVAT"
     gene            complement(1238..2035)
                     /locus_tag="VF633_11675"
     CDS             complement(1238..2035)
                     /locus_tag="VF633_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471761.1"
                     /translation="MKRRIFAIGAPNRPVLMIVAALALLSAGSCSPAKEGKAPQAPSQ
                     TQTGAGVDSGWVTPPLIESVERTASGLRVRGHAAPQGRVVLRAAGGTAYAIGADDGGR
                     FDLEIRTPATDTLFMVEAQNGQEASPAPYQMLVSHDAGGPTALLSSGAPTRRMGRAGS
                     LDVVDSDGHALLASGRATPGTSVSIAAAGRAPVQVRTGPDGRWKVILTSEGAGPAEIV
                     VAGRRYAYPGPSLGDSGALVIASNPQGWSVSWPIPPRSRQASWFPVE"
     gene            2129..>2457
                     /locus_tag="VF633_11680"
     CDS             2129..>2457
                     /locus_tag="VF633_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX8471762.1"
                     /translation="MSLPATAIAPFDGKSVCLFCGSAPGNDPAYIEAATAFGAATARA
                     GWRLVYGGGGVGLMGASARSAHEAGGRVVGIMPEFLRSRERLFDDVETIVVTSMHERK
                     TLMYDQS"
BASE COUNT          410 a          796 c          815 g          436 t
ORIGIN      
        1 gccgggtcct gatcattggt gcgggccacg caggcggttc ggttgcggcg cttcttcgcc
       61 aatatggttt cgagggcgaa atggtcctgg cgggccggga aaccgttccg ccctatcagc
      121 gtccgccgct gtccaaggca tggctcaagg gcgaggctga tctcgacgcc ttgctcctgc
      181 gtccggcgag tttttacgcg gaacagaaga tcgaactgcg caccggcgtc actgcgacga
      241 gggtcgaccc gaccagcaaa acggtgacct tcaccgatgg cacggtcgag gcttatgacg
      301 ttctcatcct cgcaacgggt tcggtggccc gtaagctgga cattcctgga gcggacaggc
      361 ccgacctcat cgaactccgc accttgcacg aggcagagcg gctgaaggcc gccttggcgc
      421 ccggcaaacg tctggttgtc gtcggaggcg ggtatgttgg tctggaggcg gccgcctcgg
      481 cacgcaccct cggcaccgag gtcgtggtga tcgagcggat ggagagggtg cttcagcggg
      541 tcgcatccga gaccctgtcg accttcttca ctgagcacca tcgttcgcat ggggtcgaaa
      601 tcaggaccga cgcagaggtc gcggggttcg atgacggcgc cgtgcggctg atagacggcg
      661 aactgatccg gtccgacgtc atcctggtcg gcgttggggc gttggcctgc gacgcgttgg
      721 cgcgaacagc cggtctcgcc tgtgagaacg gcgtagtcgt ggacgagatg gctcgaacat
      781 ccgacccgtc gatctatgct atcggggata tgacccacag accgatcccg actatgggtg
      841 ggcgcgtcat gcggctggaa agcgtgccca acgcgctgga acaagccaaa caggccgccg
      901 ccgccatcgt cggtcgtgtc gcccccgccc cggaagtccc atggttctgg tcggaccagt
      961 acgacatcaa gcttcagatc gccggcctgc ctttcggcgc tgaccgacag gtggtgcgcg
     1021 gagatcccgg ggaaggacgg tttgccgtct tccatctggt cgccgacagg atcgtctgcg
     1081 tcgaggccgt caactcacct cccgaattca tggccggacg tctgctgatc ggccgggcga
     1141 cggcggtcga caccgcaaag ctgtcggacg cttcggtgtc gatgaaagag gtcgcgacct
     1201 gaccgctccg cctgccgtgg gatcgcagga aatctgccta ttcgactgga aaccagctcg
     1261 cctgacgcga acgcggggga attggccatg acacggacca gccctgcgga ttggaagcga
     1321 tgaccagggc gccactgtct ccgagcgaag gtcccggata ggcgtagcgc ctaccagcca
     1381 caacgatttc ggcaggtccg gcgccttccg aagtgaggat cactttccat cgcccatccg
     1441 gaccggtgcg aacctgaacc ggagctcgcc ccgcagccgc aatcgatacg gacgttcccg
     1501 gcgtggcgcg cccggacgcg agaagagcgt ggccgtcgct gtccacgacg tccagcgacc
     1561 ccgcacgtcc catgcgccgt gtcggagcgc cagacgacag cagagccgtt ggcccgcccg
     1621 catcgtggga caccagcatc tgatagggtg cgggtgacgc ctcctggcca ttctgagcct
     1681 cgaccatgaa tagcgtgtct gtcgcgggcg ttcggatctc cagatcgaat cgccctccgt
     1741 catcagcgcc gatcgcgtag gcggtgccgc ctgcagcccg cagcacgacg cgtccctgcg
     1801 gggcagcatg tccgcgtacc cgtaagcctg atgccgtcct ctcgacgctt tcgatcagcg
     1861 gcggcgtcac ccaacccgaa tcgacgccag cccccgtttg ggtctgggac ggcgcctggg
     1921 gcgccttgcc ctctttggct ggggagcacg atccagcgct caggagggcc agagcagcca
     1981 cgatcatgag aacggggcgg ttcggcgcgc cgatggcaaa tatccgccgt ttcatcctgt
     2041 ccaccgggtc tgtatggctg tcggcccaga tagcccgggt taggcccggt tgtcttggcc
     2101 catcgctccg ctttccgctt gaggatccat gtccctgccc gccaccgcca tcgctccttt
     2161 cgacggcaag tccgtatgcc ttttctgcgg ctcggcgccg ggaaatgatc ccgcctacat
     2221 tgaagcagcg acggcattcg gcgcggcgac ggcccgcgcc ggttggcggt tggtgtacgg
     2281 cggcggcggt gtaggcctga tgggggcctc agcccgatcg gcccacgagg ccggaggccg
     2341 ggtagtaggc atcatgccgg agttcttgcg cagccgcgag cggctgttcg acgacgtaga
     2401 gacgatcgtc gttacctcca tgcatgagcg caagacgctg atgtacgacc agtcgga
//
LOCUS       DASVTM010000423         3829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_787358,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000423 DASVTM010000000
VERSION     DASVTM010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787358"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="VF633_11685"
     CDS             complement(<1..1613)
                     /locus_tag="VF633_11685"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX8471763.1"
                     /translation="MTLALFLGLLIKSSVVAGVGLAASRWVLSRAVDRVDVLRLTAGL
                     LAVLPLIAVFGPQFPISVPFLPLFDPVDLGASPPGPDPLWTGSTPAVAGIVASGVVWR
                     PSLVTIVASIWAIGGLIVVSRLALGVWTLQRWTRNGCPIHDLAWARQIERLDSASRPH
                     MVASSEVHSPLSWGLSPGAILIDADSLKSGSSAAAVIAHEMAHLKRADWSFLMLSRLV
                     LALFWFNPLVWWLAKSLVDRSEEAADAEAVSHVDRHAYARALISLAAGPHSAGRPAAK
                     CPMGAIAMAAQPYSLKKRIACLMNAPKADPRRPVIVGLTIATLIAVATPLAALDLHGR
                     APLPPAPPHAPVAPLPPLPPSPEASGDWVAPPVPPAPPAPPAFLAGAMDMPPAPPALA
                     VPPAPPAPPAPPARQNGQHSTHTVWSNDNGHFVITTNGVTRDATPEERVQMDAARQRA
                     TEARTIAGDARVRANQAREQARVHLASGRVDMRNGARTTHDGAAEMRRESLRLRNPAY
                     RAQQIERTRGQGRVVTDAELVAWSPRLQVR"
     gene            complement(1610..1981)
                     /locus_tag="VF633_11690"
     CDS             complement(1610..1981)
                     /locus_tag="VF633_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX8471764.1"
                     /translation="MIESLPRREREVFETLCRLEVSTSAGVRNALSDPLSDSAVRTML
                     SRLEAKGLISRSTGGDGVEYRPAQASDVVAAQALRRMVDTFFAGSAASAANALLGLGD
                     RLSPEDAAALKAMIDRAEAGK"
     gene            2179..3153
                     /locus_tag="VF633_11695"
     CDS             2179..3153
                     /locus_tag="VF633_11695"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX8471765.1"
                     /translation="MATHYLEFEKPIAELEEKIEELGLLAATSGSFETEIAGLRKKAD
                     QLRKKTYADLDPWMKTQVARHPQRPHFIDYLEGLFTDYVELRGDRQFGDDQAILGGLA
                     RFRGEAVVVIGHEKGHDTATRLTHNFGMARPEGYRKAVRLMDLAERFGLPVLSFVDTA
                     GAYPGLGAEERGQAEAIARSTERGLTLGVPFITTVTGEGGSGGAIALAAANKVLMLEH
                     SIYSVISPEGAAGILWRDGARGREAAMAMKITGPDLIGLKIVDRLIPEPLGGAHSDGD
                     ATIRAVGDAVQEELAALTLLSPAGLRKQRADRFYAIGRPADYRSPQQR"
     gene            3211..3795
                     /locus_tag="VF633_11700"
     CDS             3211..3795
                     /locus_tag="VF633_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471766.1"
                     /translation="MSSFNENLRESAVFNFNDAVTMVVDDSPFGLDLTAEALRGFGIQ
                     VRYSCISAADAINVLKDHTVDLLLIDCEMPGMDGHELVRWIRNSGLDPNAYVPIIMTA
                     GHVRKSKVGAVRDCGANYLVTKPFSAASLLERLIWVARDTRPFLEVGDYRGPDRRQRD
                     SDPRDYNERRSDMVALTEHKARALADANQNAEAV"
BASE COUNT          706 a         1211 c         1299 g          613 t
ORIGIN      
        1 cgcacctgca gacggggcga ccaggcgacc aactcggcgt cggtcactac ccgcccctga
       61 ccccgggtcc gttcgatttg ctgcgcgcga taggcgggat tgcgcagccg cagggattca
      121 cgacgcattt cggcggcccc gtcatgcgtc gttcgcgctc cattccgcat atccacacgg
      181 cctgaggcca ggtgaacacg ggcctgttcg cgtgcctgat tggctcggac ccgggcatcc
      241 ccggcgatgg tgcgggcctc ggtggcgcgt tggcgagcag cgtccatctg aacgcgctcc
      301 tcaggcgtcg cgtcgcgggt gacgccgttg gtagtgatga cgaagtggcc gttgtcgttg
      361 gaccagaccg tgtgggtcga gtgctggcca ttctggcggg cgggcggcgc aggaggcgcc
      421 ggtggcgccg ggggtacggc cagggcggga ggcgcaggcg gcatgtccat ggccccggca
      481 agaaatgcgg gtggggccgg aggtgcgggc ggaaccggag gcgcgaccca gtctccagag
      541 gcctctgggc ttggcggcag cggcggcaat ggcgcgaccg gtgcatgagg tggggctggc
      601 ggaagcgggg ctcgcccatg caagtcgagg gcggccagag gcgtcgcgac ggcgatgagc
      661 gtggcgatgg tcaggccaac gatcacaggg cggcgggggt cggctttcgg agcgttcatg
      721 aggcaggcga tccttttctt gagcgaataa ggttgagcgg ccatcgcgat ggcgcccatt
      781 ggacacttgg ctgcgggacg accggcggaa tggggaccag cggcaagtga gatcagggcg
      841 cgggcgtagg cgtggcggtc gacgtgtgag acggcctcgg cgtcggcggc ctcttccgag
      901 cggtcgacga gcgatttcgc cagccaccac acaaggggat tgaaccagaa cagggccagc
      961 acgagccgtg acagcatcag gaaagaccag tcagcgcgtt tcaggtgcgc catctcgtgg
     1021 gcgatgacag cggcggcgga tgatccggac ttcaggctgt cggcgtcgat caggatcgcg
     1081 cccggcgaca gaccccagct cagcggcgag tgaacttcgg agctggcgac catgtgcggc
     1141 cgcgaggcgc tatcgaggcg ttcgatctgc cgtgcccagg cgagatcatg gatgggacag
     1201 ccgttccggg tccagcgctg cagggtccag acgccgagcg ccagacgcga gacgacgatc
     1261 agacccccaa tggcccagat cgaggcaacg atggtcacca gcgatggtcg ccacacgacg
     1321 ccagaggcga caatgccagc gaccgcagga gtcgatcctg tccacagcgg atcgggtccc
     1381 ggcggagaag cgcccagatc aaccgggtcg aacagcggga ggaacggcac agaaatcggg
     1441 aactggggtc cgaacacagc gatcagaggc aagaccgcca gcagtccggc ggtcagcctg
     1501 agcacatcca cgcggtccac agcccggctg aggacccagc gagacgcggc aagtccgacg
     1561 cccgccacga cgctcgactt gatcagcagg ccgaggaaca gggccaaggt catttgcccg
     1621 cctccgcccg gtcgatcatg gccttgagag cggcggcgtc ctccgggctt aaccgatcgc
     1681 cgagacccag aagggcgttc gcggcgctag ccgccgaccc cgcgaagaag gtgtcgacca
     1741 tccgcctcaa ggcctgagcg gcgacgacgt cggaggcctg cgccggccgg tactcgacgc
     1801 cgtccccccc agtcgatctg gagatcaaac ccttggcttc cagccggctg agcatcgtcc
     1861 gcacggcgga gtcgctcagc gggtcagaca aggcgttgcg cacgcctgcc gaggtcgaga
     1921 cctccagacg gcacagggtt tcgaacactt cacgttcgcg cctgggcaag gattcgatca
     1981 aagcgctctc cgctacatat gtagcgccac acttgtagcg gattgcggct gagtcaaggc
     2041 tcagttcatg aactcgaaga cagggtttga ccgatcgaac ggcaacggtc tgaaccccgc
     2101 ttgccgcgaa gcattaccgg ctctaggttc cgccgcttct ctcgggcggc cctccccgct
     2161 cgcccaaccg gacgcctcat ggccacgcac tacctcgaat tcgaaaagcc catcgccgag
     2221 ctcgaggaga aaatcgagga gctggggctg ctcgccgcga cttcagggtc gttcgagacc
     2281 gagatcgcgg gcttgaggaa gaaggccgat cagcttcgca agaagaccta cgctgacctc
     2341 gatccgtgga tgaagaccca ggtggcccgc cacccgcagc gaccgcactt catcgactat
     2401 ctggaagggc tgttcaccga ctatgtcgaa ctgcgaggcg accggcagtt cggcgacgat
     2461 caggcaatcc tcggcggtct ggcgcgtttt cgcggcgagg ccgtggtcgt cataggccat
     2521 gagaagggcc acgacactgc cacccgcctg acccacaatt tcggcatggc ccgccccgag
     2581 ggctatcgca aagccgtccg gctgatggat ctggcggagc gattcggcct gccggtgcta
     2641 tcattcgtcg acaccgccgg cgcctacccg ggtctgggcg ccgaagagcg cggtcaggcc
     2701 gaggctatcg ctcgctcaac ggagcgcggt ctgacgctgg gcgtaccgtt catcaccacc
     2761 gtgacgggtg aaggcggatc ggggggagct atcgcgctcg ccgccgccaa caaggtgctg
     2821 atgctcgaac attcgatcta ttcagtgatc tcgcccgaag gcgcggccgg catcctgtgg
     2881 cgcgacggcg cccggggccg cgaggcggcc atggccatga agatcaccgg cccggacctg
     2941 ataggactca agatcgtgga tcgcttgatt cccgaaccgt tgggcggcgc ccattcggac
     3001 ggcgacgcga ccatcagggc cgtcggcgat gcggttcagg aggagcttgc ggccctcact
     3061 ctcctgtcgc ctgccggttt gcgcaagcaa cgcgctgacc ggttctacgc catcggaagg
     3121 ccggctgatt acaggtctcc gcaacagcgt taacggcttc tccaccgtcg gctgacacct
     3181 tcgggccgtg attcactttt cgctacgatc atgagcagtt tcaacgagaa cctgcgcgag
     3241 agcgcagtgt tcaacttcaa tgacgccgtg accatggtgg tcgacgattc tccgttcgga
     3301 ctggacctga cagcggaggc cctgcgcggg tttggcattc aggtccgata ttcctgcatc
     3361 agcgccgccg acgcgatcaa cgtgttgaaa gaccacacgg tcgacctgtt gcttatcgat
     3421 tgcgaaatgc cgggcatgga cggccacgag ctggtgcgct ggattcgaaa ctcgggtctg
     3481 gatcccaacg cctacgtgcc aataatcatg acggctggcc acgtccggaa gtcgaaagtc
     3541 ggcgctgtgc gcgattgcgg cgcaaattat ctcgtcacca agcccttcag cgccgcgagt
     3601 ttgctggaac gcttaatctg ggtggcccgg gacacccgcc ccttccttga agtgggcgac
     3661 tacaggggcc ccgaccgccg tcagcgagat tccgatccgc gcgattacaa tgagcgccgc
     3721 tccgatatgg tcgctcttac cgaacacaag gcgcgcgcgc tcgccgatgc aaaccagaac
     3781 gcagaggctg tctgatggtc gtcgtcgtga gacatgagcg aaagtcgcg
//
LOCUS       DASVTM010000424         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_145070,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000424 DASVTM010000000
VERSION     DASVTM010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145070"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF633_11705"
     CDS             complement(<1..402)
                     /locus_tag="VF633_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8471767.1"
                     /translation="MRITFHRIAYEALDICNALEMATLEAAVVVTGLEPGARALDIGS
                     GNGAVAIRLAERFGFRVEAVELDQAMAELARSRIAGSASRGQIILHETRSDQVLNAGG
                     PFDLIVALGVTEPVGQGIRDPRGMFDGLKQHL"
     gene            512..1084
                     /locus_tag="VF633_11710"
     CDS             512..1084
                     /locus_tag="VF633_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471768.1"
                     /translation="MTATIYSNASSVSAEPALAVCAGRVLILAGAVFASANLFQFGVM
                     SGLLHLSPAALSLSWPVALAVFFTVLARLRRGGSVNVRRVTTWSRMAIGAQIVVAVVL
                     LAVSFLRHDFTFMYWTSPIGLIVYGSAWMTTAIRGGRRWMIAPSLGAFAAAGVVISLL
                     GTAPAYLAYAAGLVAFVLIPGLWLTLGHKA"
     gene            1108..1758
                     /locus_tag="VF633_11715"
     CDS             1108..1758
                     /locus_tag="VF633_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471769.1"
                     /translation="MTDDLDTRPSDLTPDIAWIRSLAEEGSNAPLGGGRVLLAAGLIY
                     GAASLFQWASIENLVPFSPRQANLGWGVATILFLAFTLIDRVFLCPPTGVKTAANRAA
                     RAAWSCVGAGIFAGIASMVAVSWRLEDAGYGLLWLLPSMVMVFYGLGWGVSAAMIRSQ
                     RLVWLSVASFVSAPLLALMTGTAQQLLAYAACLFLLMAVPGFLLMRAASAARTTTA"
     gene            1758..2060
                     /locus_tag="VF633_11720"
     CDS             1758..2060
                     /locus_tag="VF633_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8471770.1"
                     /translation="MAEDFDIGRIDDVIHGRVRLGIMAVLAGVDSADFNTLKARLQTT
                     DGNLSVHLRKLEDAGFVAVEKSFQGRKPLTQARMTEAGRKAFLGYLDAMAGLVRDR"
     gene            2080..2859
                     /locus_tag="VF633_11725"
     CDS             2080..2859
                     /locus_tag="VF633_11725"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX8471771.1"
                     /translation="MTDRLHRLETLDNLRDYGDYATADGGRIRRGHLIRSANQARMSD
                     ADLEALAAYGFGTIVDLRRPIERTKQPSRRYVGFDGRVIENDLDAVGEAPHITFLKSA
                     DLTPDSGRRFMTALYAEMAFAPSHLEIFSRYFEVLADSDRPVLIHCAAGKDRTGMLAA
                     LTHHLLGVSRDDLLEDYLLTNVAVDLEARAPDMALQLQKMTGRLAAPDAVVAFMGVEP
                     AYLDAAFKAIEARHGSVDFYLEQALGVDAGRRDRIIERLTA"
     gene            3000..>4029
                     /locus_tag="VF633_11730"
     CDS             3000..>4029
                     /locus_tag="VF633_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX8471772.1"
                     /translation="MAGYPDRVLIVETTDEALFAPLETDEWRGGGVVGLASYDAGARS
                     ATGSRPSVWPDLILARYPAIAVFDHDASRLTVIGWGETEADADAACSVAEVWISRAAD
                     PDRPDAPARELIPDAPPQTYLDAVAEVVDRIAAGDLFQANIARGWNGTLNAGADPFDV
                     FLRLTQARGATARSAPYGAFWRAGDRALVSNSPELFLACNAATGRIETRPIKGTRPRD
                     QDPAIDAVNATELLASAKDRAENLMIVDLMRNDLARVCRPGSVMVDRLFEIESHSTVH
                     HLVSTVTGLLNPETGSAAVLEAAFPPGSITGAPKHQAMKVIATLEPPRGPWCGSLFLI
                     DEAGDLTAS"
BASE COUNT          629 a         1399 c         1238 g          763 t
ORIGIN      
        1 cagatgttgc ttcagcccat cgaacatccc gcgtggatcg cgaatgcctt ggcccaccgg
       61 ctcggtgacg cccagcgcca cgatcaggtc gaacggaccg cctgcgttca gcacctgatc
      121 cgaccgcgtt tcgtgcagga tgatctggcc gcgcgacgcc gagcccgcga tccgcgatct
      181 ggccagttcg gccattgcct ggtctagctc gaccgcctcc acgcgaaacc cgaaccgctc
      241 ggccagacgg atggccacgg ctccattgcc gctgccgata tccagcgccc gcgcgccagg
      301 ctccagtcct gtgacgacga ccgccgcctc cagcgttgcc atctccaggg cattgcagat
      361 gtcgagcgcc tcataggcga tccggtggaa ggttatgcgc atccccatcg ataacccgcg
      421 tgtccccgca tctccaaaca gatcaaagaa agcactttac gtttaaaagt acatcgcata
      481 tgagtgggct atcaacaccg gggagccagc tatgaccgcc accatctatt ccaatgcatc
      541 ttctgtctct gccgaacccg cgcttgctgt ctgcgccggt cgcgtcctga tccttgcggg
      601 cgccgtcttc gcctccgcca accttttcca gttcggtgtc atgagcggcc ttttgcacct
      661 gtcgcccgcc gcactgtcgc tgagctggcc cgtcgcgctg gccgtcttct tcaccgtcct
      721 cgcccgcctg cgcaggggcg gcagcgtcaa cgtccgtcgg gtcaccacat ggtcccgcat
      781 ggccatcggc gcccagatcg tcgtcgcagt cgtactgctg gcggtgagtt tcctgcggca
      841 cgacttcacc ttcatgtact ggacctctcc gatcggtctg atcgtctacg gttcggcctg
      901 gatgacgacg gcgatacgcg gtggtcgtag atggatgatc gcgccttcgc tcggcgcgtt
      961 cgccgccgcc ggagtcgtga tctcactgct ggggaccgca ccagcctacc tcgcctacgc
     1021 cgccggcctc gtcgccttcg tcctcatccc cggcctgtgg ctgacattgg gccataaggc
     1081 ctgaatcgtt ctagagaact cacaaccatg accgatgacc tcgatacccg cccgtccgac
     1141 ctcacccccg acatcgcgtg gatccgcagc ctggcggagg aaggttccaa tgcgccgctc
     1201 ggcggtggcc gggtcctgtt ggccgccggc ctgatctacg gtgcggccag tcttttccag
     1261 tgggcgtcga tcgagaacct cgttccgttc agccccaggc aggcgaatct gggatggggc
     1321 gtagcgacta ttctgttcct ggccttcacc ctgatcgacc gggttttcct ctgcccgccg
     1381 accggcgtga agaccgcggc gaaccgagcg gcgcgcgcgg cctggtcctg cgtcggcgca
     1441 ggcatattcg cgggcatcgc ctccatggta gctgtcagct ggcggctcga agatgcaggc
     1501 tacggcctgc tgtggctgct gccctccatg gtcatggtct tctacggcct cggctggggc
     1561 gtctcggcgg cgatgatccg atcgcaacgg ctggtgtggc tctcggtcgc ttcgttcgtc
     1621 tccgccccgc tgctggcgct gatgaccggg actgcgcaac agctcctcgc ttacgcggcc
     1681 tgcctgtttc tgctcatggc cgtgcccggg ttcctgctga tgcgagccgc aagcgccgcc
     1741 cggacgacga cagcctgatg gccgaggatt tcgacatcgg tcggatcgac gacgtcatcc
     1801 atgggcgggt gcgtctgggc atcatggccg tgctggcggg cgtcgacagc gccgacttca
     1861 acaccctgaa ggcccgactg cagaccacgg acggcaacct ttcggtccac ctccggaaac
     1921 tggaagatgc cggtttcgtc gccgtcgaga aaagctttca gggtcgcaaa ccgctgacac
     1981 aggcccgcat gacggaggcc ggtcgcaaag cctttctcgg ctatctcgac gccatggccg
     2041 ggctggtgcg cgaccgctga caggccctat agatccggca tgaccgatcg tctgcaccgc
     2101 ctcgaaaccc tcgacaacct ccgcgactac ggcgactacg ccacggccga cggtgggcgg
     2161 atcaggcgcg gtcacctgat ccgttcggcc aatcaggctc ggatgagcga cgccgatctt
     2221 gaagcgctcg cggcctacgg gttcggcacc atcgtcgatc tgcgccgccc gatcgaacgc
     2281 accaaacagc cgtcgcgtcg atatgtcggc ttcgacggcc gggtgatcga gaacgacctc
     2341 gacgccgtgg gcgaggcccc gcacatcacc ttcctcaagt ccgccgatct gacaccggat
     2401 tccggccggc gtttcatgac tgccctctat gccgagatgg ccttcgcgcc gtcacacctc
     2461 gagatcttca gccgctattt cgaggtgctg gccgacagcg accgcccggt cctgatccac
     2521 tgcgcggcgg gcaaggaccg caccggcatg ctggccgccc tgacccacca cctgctgggc
     2581 gtctcgcgcg acgatctgct cgaggactat ctgctgacaa atgtcgcggt cgatctcgag
     2641 gcccgggcgc cggatatggc cctgcaactg cagaagatga ccggtcggct cgccgcccca
     2701 gacgccgtcg tcgccttcat gggggttgaa cccgcctatc tggacgccgc cttcaaggcc
     2761 atcgaggcgc gccatggctc tgtcgatttc tatctggaac aggcgttggg cgtggacgcc
     2821 ggacgtcgag accgcatcat cgagcgactt acggcatgac cgggccgagc gggcgcgcgg
     2881 tgcaggtccg acgcgtcctc ccctgggtat cgccgcttta tgtggcgatg ggggtcgctg
     2941 acagcgacgg cgcactgtgc ctcctttcgg accctgacgt tcccgccagc cgctctttcg
     3001 tggcggggta tcccgatcgg gttctgatcg tagagaccac tgacgaggcc ttgttcgctc
     3061 ccctggagac tgatgagtgg cggggcggcg gcgtggtcgg gctggcgagt tatgatgccg
     3121 gggcccgttc cgcgaccgga tcgcgccctt cggtttggcc ggacctgatc ctggcccggt
     3181 atccggcgat cgcggtgttc gaccacgacg cctctcgact aaccgtcatc ggctggggag
     3241 agaccgaggc cgacgcagac gcagcctgta gcgtcgcgga agtctggatc tcgcgcgcag
     3301 ccgaccccga ccgtccggac gctccggcgc gcgaactgat ccctgacgcg ccgccgcaga
     3361 cctatctcga tgcggtcgcc gaggtcgtcg accggatcgc cgccggagac ttgtttcagg
     3421 ctaatatcgc gagaggttgg aacggaaccc tgaatgcagg cgccgacccg ttcgacgttt
     3481 tcctccggct tacacaggcg cgaggcgcga ctgcgcgttc cgccccttac ggcgcctttt
     3541 ggcgagctgg agacagggcg ctcgtatcga attcaccgga gcttttcctc gcctgcaatg
     3601 cagcgacggg gcgcatagag acccgcccga tcaagggcac ccgtccacgc gaccaggacc
     3661 ccgcgatcga cgcagtcaac gcgaccgaac ttctggctag cgccaaggac cgggccgaga
     3721 acctgatgat cgtcgacctg atgcgcaacg atctcgcccg cgtctgccgg cccggctccg
     3781 tcatggtcga ccgcctgttc gagatcgaga gccattcgac ggttcaccat ctcgtctcca
     3841 ctgttacagg ccttttgaac cctgaaaccg gttccgctgc agttctggaa gcggcctttc
     3901 cgcctggctc catcaccggc gctcccaagc atcaggccat gaaggtcatc gccacactgg
     3961 agccgccgcg ggggccttgg tgcggttcgc tgtttttgat cgatgaagca ggcgacctga
     4021 cggcctcgg
//
LOCUS       DASVTM010000425         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_465567,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000425 DASVTM010000000
VERSION     DASVTM010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465567"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1466)
                     /gene="astD"
                     /locus_tag="VF633_11735"
     CDS             complement(48..1466)
                     /gene="astD"
                     /locus_tag="VF633_11735"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEX8471773.1"
                     /translation="MTHFTSTNPATGETVWEGEAASPAEASAAVESARAAFPDWADRP
                     RQERIDAMKRYQSVLKRRAPEMAEAISRETGKALWETTAELGSMAGKVDISIRAYDER
                     TGERTTETAFGHATLRHRPHGVAAVLGPFNFPGHLPNGHIVPALLAGDTVVFKPSEET
                     PHTGQLMAECVAEADLPAGVFNLLQGGRETGGALLDQPIDALMFTGSGAAGGYFRKKF
                     ADDPHVILALELGGNNPLVVWDAADAEAVAGIVVQSAFVTTGQRCSCARRLVVPEGPK
                     GDAIIEAVAALSDRLVFAPWDSASEPYAGPLISEKAANAALQALQQRIDLGAKVIRAS
                     GPVHNLPGSFVRPAIIDVTGLKVLDEEMFAPFLSVARVPTFEAAIGAANDTRYGLSAG
                     LVSDDPRNWDRFIRRIRAGVVNFNRPTTGAAGDMPFGGLGDSGNHRPSAWYAADYCAY
                     PVASFEAGAVKNIEGEIKGLRQ"
     gene            complement(1474..2481)
                     /locus_tag="VF633_11740"
     CDS             complement(1474..2481)
                     /locus_tag="VF633_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEX8471774.1"
                     /translation="MLVVRPAGPGDLDFLLELAILSGPGFTSLPEDPDQLTERLDLSR
                     DSFSGSLAARECWYTLMLEETDTGDVDGIASVKAAVGLHRPFFSFRVNTNASSSPSLD
                     IKLEHQTLQLVNECSGWTEVGSLFLKADRRKGGAGRLLSQSRYMLIGAQPDLFAESVL
                     AELRGVFTPDGACPFWDHVAHKFFPMPFDQADMLTGSTDKQFILDLAPRHPIYVELLP
                     EAARAVIGKVHPQGVPAMALLESEGFRPNGLVDIFDAGPTVSCGRDNIRTVRDARSMT
                     VAIADEVEAELPSLISTDSVGDFRAVRQRVALEGSVATLSAEAADALKVRVGDTVRIK
                     A"
     gene            complement(2482..3732)
                     /locus_tag="VF633_11745"
     CDS             complement(2482..3732)
                     /locus_tag="VF633_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX8471775.1"
                     /translation="MRILSPDQTVLDHVAAREASIIGRAVDWAGVNSGSRNAGGLNTM
                     LDLLDEAARRLPGEVVRIPTQGSTMVGDDGSVKIEAHTDALKITARPHAPVQVVLTGH
                     YDTVFPAESAFQSVKSRSDGALNGPGIADMKGGISVMLAALEAFETHPDRTNVGWTVL
                     LSPDEEIGSPASAPLLAQLGAAGHVGMTYEPALADGTLAGARKGSGNFHLIVTGKAAH
                     AGRAFHEGANAVAGAAIIAAALHALNGRREGVTVNVAKISGGGALNVVADNAVVRFNV
                     RVPDVQSAAWVTDAVHEIATTPPFEGLTLDLHGGMTRAPKPMDASQTALFEAVRDAGS
                     LLGQSIAWSPSGGVCEGNNLHAAGLPNIDTLGVRGGDIHSDQEFAWPDSFVERAQLSA
                     LILCKLASGEIDAHRLKSLRLETM"
     gene            3833..>4299
                     /locus_tag="VF633_11750"
     CDS             3833..>4299
                     /locus_tag="VF633_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471776.1"
                     /translation="MSLISAMNPTAPITTEAEAQAAARSSAIAIVIGVIYGVIGVIIT
                     YASMASLKAAATAAAAANGTEGGPDAAMIANVAIQSALWMGIGFTVIQAILAFVQWRR
                     PNKVIAIIFLALIALGFVSLIFTMVMANSGAMPSTSPATPIWQVLLGLVVMAV"
BASE COUNT          711 a         1494 c         1380 g          714 t
ORIGIN      
        1 atccgtcagc cttcggctgc caccttctcc cgcaagggga gaaggagtca ttgccgcaac
       61 cccttgatct cgccctcgat gttcttcacc gcacccgcct cgaaactggc caccggatag
      121 gcgcagtaat ccgccgcgta ccaggcgctc ggccggtggt tgccactatc gccgagaccc
      181 ccgaacggca tgtcgcctgc tgcgcccgtc gtcggtcggt tgaagttgac cacgcccgcg
      241 cggatacggc ggatgaaccg gtcccaattc ctcggatcgt ccgacaccag acctgccgac
      301 agcccgtagc gggtgtcgtt ggcggctccg atggcggcct cgaatgtcgg cacgcgcgcc
      361 accgacagga atggcgcgaa catctcctcg tccagcacct tcagtccagt gacgtcgatg
      421 atcgcaggcc tgacgaacga gcccggcagg ttgtggaccg gccccgacgc gcggatcacc
      481 ttggccccca gatcgatccg ctgctgcagc gcctgcaacg ccgcgttcgc cgccttctcc
      541 gagatcaacg gcccggcata gggctcactc gccgagtccc acggcgcgaa caccagccgg
      601 tcgctcagcg cggcgacggc ttcgatgatc gcgtcgcctt tcggtccttc gggcacgacc
      661 agtcgtctcg cgcaggagca gcgctgcccc gtcgtcacga acgccgactg caccacgatc
      721 ccggccaccg cctcggcgtc cgctgcatcc cagaccacca gcggattgtt gccgcccagt
      781 tccagcgcca ggatcacatg cggatcgtcc gcgaacttct tcctgaaata cccgcctgcc
      841 gcgcccgagc ccgtgaacat cagggcgtcg atcggctggt ccagcaaggc gccgccggtc
      901 tcgcgcccgc cctgcaacag gttgaacacc ccggccggca ggtccgcctc ggccacgcat
      961 tcggccatca gctgaccggt atgcggtgtc tcctccgacg gcttgaacac cacggtgtct
     1021 cccgccagca gcgccgggac gatatggccg ttcggcaggt ggccggggaa gttgaagggg
     1081 ccgagcactg ccgcgacccc atgtggtcgg tgccgcaggg tcgcatggcc gaacgcggtc
     1141 tcggtcgtgc gctcgccggt gcgctcgtcg taggcgcgga tcgagatgtc gaccttgccg
     1201 gccatcgagc cgagttcggc ggtggtctcc cacagcgcct tgccggtctc gcggctgatc
     1261 gcctcggcca tctcgggcgc gcggcgtttc agcaccgact gatagcgttt catcgcatcg
     1321 atccgctcct gacgcgggcg gtcggcccag tcggggaagg cggcgcgtgc gctctcaacg
     1381 gcggcgctgg cctcggcggg ggaggcggcc tcgccctccc agacggtctc gccggtggcg
     1441 gggttggtcg aggtgaaatg ggtcatgggg aactcaagcc ttgatacgaa cggtgtcgcc
     1501 gacccgcacc ttcagggcgt cggcggcttc ggcggaaagg gtggccacgc tgccctccag
     1561 cgccacgcgc tgacggacgg cacggaaatc gccgacgctg tcggtcgaga tcagcgacgg
     1621 cagttcagcc tcgacctcgt cggcgatggc cactgtcatg gatcgagcgt ctcgcacggt
     1681 gcggatgttg tcgcgaccgc aagacaccgt cggcccggca tcgaagatat caaccagacc
     1741 gttgggccgg aagccctcgc tttccagcag cgccatggcg gggacccctt gcggatgcac
     1801 cttgcctatc accgcccgcg cggcctccgg cagtagttcg acatagatgg ggtggcgagg
     1861 agccagatcc aggatgaact gcttgtcggt cgagccggtc agcatgtccg cctgatcgaa
     1921 cggcatgggg aagaatttgt gcgccacatg atcccagaac gggcaggcgc cgtcgggcgt
     1981 gaacacgccg cgcagttcgg ccagaacgct ctcggcgaac aggtcgggtt gggcgccgat
     2041 cagcatatag cgcgactggc tgagcaaccg tccggccccg cccttgcgcc ggtcggcctt
     2101 gaggaacagc gacccgacct cggtccagcc gctgcattcg ttgaccagtt gcagggtctg
     2161 gtgttccagc ttgatgtcca gcgacggcga ggacgaggcg ttggtgttga cccggaacga
     2221 gaagaacgga cggtgcaggc cgaccgcagc cttaacgctg gcgatgccgt ccacgtcgcc
     2281 ggtgtcggtc tcctcgagca tcaaagtgta ccagcactcg cgagccgcca gcgatccgct
     2341 gaagctgtcg cggctgaggt cgagccgttc ggtcagttgg tcgggatctt caggcaggct
     2401 ggtgaaaccg ggccccgaca ggatcgccag ctccagcagg aaatcgagat cgccgggacc
     2461 ggcaggacgg acgaccagca tctacatcgt ctccagacgc agggatttca ggcgatgcgc
     2521 gtcgatctcg cccgacgcca gcttgcacag gatcagggcg ctcaactgcg cccgctcgac
     2581 gaagctgtcg ggccaggcga actcctgatc cgaatggatg tcgcccccac gaacgcccag
     2641 cgtgtcgatg ttgggcaggc cggcggcatg aagattgttg ccttcgcaca ccccgcccga
     2701 cgggctccat gcgatggatt gtcccagcag ggagccggcg tcacgcaccg cctcgaatag
     2761 cgcggtctgg gaggcgtcca tcggcttggg cgcgcgggtc atgccgccgt gcaggtccag
     2821 cgtcagccct tcgaacggcg gcgtcgtggc gatctcatgc accgcatcgg tgacccaggc
     2881 ggcggattgc acatcgggaa cgcgcacgtt gaaccgcacc acggcgttgt ctgcgaccac
     2941 gttcagggcc ccgccgccgc tgatcttggc gacattcacg gtcaccccct cgcggcgacc
     3001 gttgagggca tgaagcgcgg cggcgatgat cgccgcgccg gcgacggcgt tggcgccctc
     3061 gtggaaggcg cggcccgcat gggcggcctt gccggtgacg atcagatgga aattgccgct
     3121 gcccttacgt gcacccgcca gcgtcccgtc ggccaacgcc ggctcatagg tcatgccgac
     3181 atgccccgcc gcacccagtt gcgccagcaa cggcgcggag gcaggcgagc cgatctcctc
     3241 gtcgggggac agcagcacgg tccagccgac gttggtgcga tccgggtggg tctcgaacgc
     3301 ctccagagcc gccagcatca ccgaaatgcc gcccttcatg tccgcaatcc ccgggccgtt
     3361 cagggctccg tccgaccggg atttcaccga ctggaaggcg ctctctgccg ggaacaccgt
     3421 gtcgtaatgc ccggtcagca cgacctgcac cggcgcgtgg ggccgcgccg tgatctttaa
     3481 cgcgtcggtg tgagcctcga tcttcaccga cccatcatcg cccaccatgg tcgagccttg
     3541 tgtcgggata cgcaccacct cgcccggcaa ccgccgcgcg gcttcatcca gcaggtcgag
     3601 catagtgttc agcccgccgg cgttgcggct gccggagttg accccggccc agtccaccgc
     3661 ccgaccgatg atcgaggcct cccgcgccgc cacatggtcg agcacggtct gatcgggaga
     3721 aagaatccgc atcgtccaag cctaggcctc cgcgtcgaaa aggtgaaggt cggcggtttc
     3781 gcaacggcgt tccgcaagct aggattgtcg caaatcaatg gagaggaaga ccatgagcct
     3841 gatcagcgca atgaacccga cggcccccat caccaccgaa gccgaggccc aggccgcagc
     3901 gcgttccagc gccatcgcca tcgtcatcgg cgttatctac ggcgtgatcg gcgtcatcat
     3961 tacctacgcc tccatggcgt cgctcaaggc tgcagcgact gccgcagccg ccgccaatgg
     4021 aaccgagggc ggaccggacg ccgccatgat cgccaacgtg gccattcaaa gcgctctctg
     4081 gatgggtatc ggctttaccg tgattcaggc gatcctcgcc ttcgtgcaat ggcgtcgtcc
     4141 aaacaaggtg atcgccatca tcttcctggc tctgattgcg ctcggcttcg tcagcctgat
     4201 ctttacgatg gtcatggcca actctggcgc catgccctcg accagcccgg cgactccgat
     4261 ctggcaggtc cttctcggcc ttgtggtcat ggcggtcga
//
LOCUS       DASVTM010000426         9286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_359450,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000426 DASVTM010000000
VERSION     DASVTM010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359450"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VF633_11755"
     CDS             <1..463
                     /locus_tag="VF633_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471777.1"
                     /translation="PIPGTELMRITSVAAIGVLAAGMLAGCSTQTKAPFDQGVCYSVA
                     LPKDGEKTAPRFNVLARDQAQIEFCAAKLEIMRVQFLRLGGSTREIVGAYQGQFIFID
                     RGGVSFGRTLEGQRFFALTRTGDGRLAIPGAIQRSIDTAETPAPAAAQPAG"
     gene            656..1168
                     /gene="ssb"
                     /locus_tag="VF633_11760"
     CDS             656..1168
                     /gene="ssb"
                     /locus_tag="VF633_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8471778.1"
                     /translation="MAGSVNKVILVGNLGRDPEIRSMPNGDRIANFSLATSETWRDKS
                     SGERKEKTEWHRVSVFNDNIVKVVENYVKKGSTVYVEGSLQTRKWTDKDGAEKYTTEI
                     IIGRFNGQLTMLGGRDGGGGGSQGGGGGGGGGAGGRGDDDYSSSFSTGGANKPSGPRE
                     SYDLNDDIPF"
     gene            1313..1966
                     /locus_tag="VF633_11765"
     CDS             1313..1966
                     /locus_tag="VF633_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471779.1"
                     /translation="MPALPQALKFPIFATKEDAATALGGLVVKLSYADLDEKSAYKAK
                     FAGTPPLKVSNWASGGCVGALQTAGNVWTFKDKGVADFFVALFEEVRAGANARLARAD
                     LFHGHINFYKGTLLIVFHAAEYPIDLLDTKAPAKNAALVEPGVTIKATDANFRYRNAI
                     YCFSTGILETLDTQKLDAKWKDNFDPKSITLLDDTLGSSVAGLNLFSTEGIPFPFLA"
     gene            2085..2987
                     /locus_tag="VF633_11770"
     CDS             2085..2987
                     /locus_tag="VF633_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8471780.1"
                     /translation="MTHDKPHSPLSLLEIGAILVIIVTWGVNNAAGKLATETMPPLMV
                     GALRFAIATICLAGFVRPPFPDWKSLVLIVVIGGAVQYGLLYTAYWLAQDVSPATVAN
                     QLWIPMTSLFAFLLLKEKLSRAAMVGMAIAFAGVGWMMLDSHALQDWRAILVAIAAGA
                     AWALTTVVARRTTSIPPLKMQGLLAAGTLPIMAFGSAVFEHGQIEAVRHATPLVWASL
                     FWAGVISSVFATTLLFWLVQKREAGRVTPYLLATPVVTILIGWLFMGDVLTPQILTGA
                     ALAMGGVAVVALAERGLRAGAAKA"
     gene            complement(2995..3597)
                     /locus_tag="VF633_11775"
     CDS             complement(2995..3597)
                     /locus_tag="VF633_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX8471781.1"
                     /translation="MRALPLFAALAASSILGAGAALAQTTPAAPAAAPTPAPAPVPVA
                     VATAALKTADGADAGTVTSFEGPKGMIFRIDGKGWPAGWHAVHLHAVGTCEGPAFTSA
                     GGHVNHAEGARPHGLLNKSGGPDLGDLQNVYAAADGSVHADVYLAWEGLNAKPSDYID
                     ANGLSFLVHASPDDYVSQPIGGAGARIACGVFQSAAHTGH"
     gene            3719..6541
                     /gene="gyrA"
                     /locus_tag="VF633_11780"
     CDS             3719..6541
                     /gene="gyrA"
                     /locus_tag="VF633_11780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8471782.1"
                     /translation="MTDDSYENAAPPANSGGSGSGGGSDISTITIEDELKRSYLDYAM
                     SVIVSRALPDARDGLKPVHRRILYSMHDLNMTPDRSYSKCARVVGDVLGRFHPHGDAS
                     VYMALVRMAQPFSMGLMLVDGQGNFGSVDGDMPASMRYTEARMAPAAVALLADIDKDT
                     VDFQPNYDEKELEPVVLPARIPNLLVNGAGGIAVGMATNIPPHNLGEIVDAAVALLDN
                     ADISDDALLDIVPGPDFPTGGEIMGRTAPRNALRDGRGSVIVRGKASIETIRKDREAI
                     VVTELPFQVNKQTLIERIAEMVREKRIEGISDVRDESDRQGMRMVIELKRDASGDVIL
                     NQLWRYTAMQSSFGVNMLALNHGRPEQMGLRDLLTIFLDFREEVVVRRVKFELAKARD
                     RGHVLVGLAVAVANIDEVIHIIRSSADPAEARQRLQAKPWPVADMIALVELIADPRSM
                     LIDGDKLNLTDEQARAILALTLSRLTGLGRDDIFGEARGLADTIQGHLTILSDRANIL
                     AIIREDLLLVKTEFAVPRRTTIGEGDAEMEDEDLIPREDMVVTVTHGGYVKRVALNAY
                     RTQHRGGKGKSGMTMKDEDAITGVFSASTHTPVLFFATNGKAYKLKVWRLPLGGPTSR
                     GKAFVNLLPIEPGDSIMNVLPLPEDETTWGDYDIMFATKSGDVRRNKLSDFASVNRAG
                     KIAMKLEGDDHMVGVALCTAEDDVMLTTALGRAIRFKADDVRVFKGRDSTGVRGVRLQ
                     SGDEVISMAVLGRVDASPEERTAYVKHANAMRKAMGEGDDETAVVEADEDGAEEGGEA
                     VLSVERIAALGAAEQFILTVTDTGFGKRSSAYEYRRTGRGGQGLTAHGLGGRAGKRLA
                     AAFPVEDTDELLMVTSTGQMIRTRVGQVRVVGRSSQGVTIFRTAEGERVVSVERLAET
                     PGGDEDLDLDTGGDDGVEGTEDGGEN"
     gene            6546..7349
                     /locus_tag="VF633_11785"
     CDS             6546..7349
                     /locus_tag="VF633_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX8471783.1"
                     /translation="MTGFPPILVDGEPISTNDLIYLALVNYAAYTSFRVEAGAARGLS
                     RHLDRLHASALELFGESPGKARFRDLMRTAVEGHEACWLRVSLFSPELWARTPSWRGM
                     PKVMTMAAQPPPPLAASLRLATRVYSRDAAHLKHTAVFGLIQSRRQAQDEGFDDALFV
                     DKAGLISEGSLWNVGFVRGDRVTWPQAPMLSGVTQGLIEQGLADVGLTAETRPVKLSD
                     LPDFDHAFICNSATPACAVTAIDGHGFESDPALIERLHAAWSFNRPEAI"
     gene            complement(7352..8938)
                     /locus_tag="VF633_11790"
     CDS             complement(7352..8938)
                     /locus_tag="VF633_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8471784.1"
                     /translation="MNRRELSFGIATAAIASAAGSAQAAQYRRSQPRVAIPAVAATRG
                     GQGVYAPQIARLPATLQDDVRKFYEMNGWKTVWAPQQVQALNAVARKSENHGLPATDF
                     FDFVGLAADPASAEVRTTAAAISYAQVLAEGRVRPEDVEDLWEMDKNRVDLPTGLNNA
                     LAQNQLVQWFDGLPPTDIGYTNLSNGYLRYRRLSRAGGWPTFTQGASIEPGMSDRRIP
                     VLIDRLVAEGDVSAADGARLKAQGLTYGDELQTAVRGFQQRHGLLADARLGAGTQRSL
                     GASSDDRARQIALNLERRRWLKRELSPERIEVNTAAAIMVYWKDGRPVHSNRVVVGSA
                     ENQTPSLEKPFASVVANPPWYVPAGIARREILPKGPAYMAARDMYVSNGQVIQRAGPQ
                     AALGYVKFELRDSYAIFLHDTPSKGAFDLATRQRSHGCVRVQGAVEFARLLLSPDPVR
                     LSEFDTAQITRDTQRIQTGREITVRLLYWTAFVDGQQRVAFREDVYKRDQKLAEALGI
                     AVSLPVPIDDGAAVANDVGP"
     gene            complement(9084..>9286)
                     /gene="rpmE"
                     /locus_tag="VF633_11795"
     CDS             complement(9084..>9286)
                     /gene="rpmE"
                     /locus_tag="VF633_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX8471785.1"
                     /translation="YHFITVTLTNGDTYQTRSTYGKEGDVLNLDIDPSTHPAWTGGNQ
                     QMLDRGGRVSRFNNKFGGFIKK"
BASE COUNT         1684 a         2908 c         3027 g         1667 t
ORIGIN      
        1 gccaattccc gggactgaac tgatgcgcat cacatccgta gcagccatcg gcgttctggc
       61 tgccggcatg ctcgccggct gctccaccca gaccaaagct cccttcgatc agggcgtttg
      121 ctacagcgtc gccctgccga aagacggcga gaagaccgcg ccgagattca atgtactggc
      181 tcgagatcaa gcacagatcg agttctgcgc cgccaagctg gagatcatgc gagtgcaatt
      241 cctgcgtctt ggcggatcta cccgcgagat cgtcggcgcc tatcaggggc agttcatctt
      301 catcgaccgg gggggggtgt ccttcgggcg cacccttgag gggcagcgct tcttcgctct
      361 gactcgtacg ggcgatggcc gtctggcgat tcccggtgcg atccagcgta gcatcgacac
      421 cgccgaaaca ccggcgccgg cggcggctca acccgcaggc tgaggccgca agggttctcc
      481 ggcgagaaca aagctggaac atccacaagc tttcgtttag attgtcttaa tactgggtgg
      541 cgtcggggcc caggcgcgct acgccggacg actgacggcg gggtcgccgt acgagacaat
      601 caatcaagcc gcgtcctgat gaagaggcgc ggggcagaca acgagagaga acatcatggc
      661 gggcagcgtc aacaaggtca tcctggtcgg taatctgggg cgcgaccccg aaatccggtc
      721 gatgccgaac ggcgaccgga tcgccaactt ctccctggcc acttcggaaa cctggcgcga
      781 caagtcctcg ggcgagcgca aggaaaagac cgaatggcac cgcgtctcgg tcttcaacga
      841 caatatcgtc aaagtcgttg aaaactatgt gaaaaagggc tcaaccgttt acgtcgaagg
      901 ctccttgcag acgcgcaaat ggaccgacaa ggacggcgcc gaaaaataca ctacagagat
      961 catcatcggt cgcttcaacg gccagttgac gatgctgggc ggccgtgacg gcggcggggg
     1021 tggttcgcag ggcggcggcg gcggcggcgg cggtggggct ggcggtcgtg gcgacgacga
     1081 ctactcgtcc agcttctcca ccggcggcgc caacaagcct tccggtccgc gcgaaagcta
     1141 cgacttgaac gacgacatcc cgttctaagg gttcttgccg gcccggagat cgcgtggccg
     1201 tgcggatcgg ttcgcggagc gcgcatgacg ccacgcgatc tcgctataaa gttgtataca
     1261 aaatcaacct gagggtgtgt aaggggtcga tcccatagcc aggacgtcat ccatgcccgc
     1321 tcttccacaa gccctcaagt ttccgatctt cgccacgaaa gaggacgccg cgaccgcctt
     1381 gggcggtcta gtcgtcaagt tgtcgtatgc cgatttggac gagaaatcag cctacaaggc
     1441 aaagttcgcg ggcaccccgc cgttgaaggt atccaactgg gccagtggcg gttgcgtggg
     1501 cgcgttgcag acagccggca atgtctggac gttcaaggac aagggcgtcg ccgatttctt
     1561 tgtggccttg ttcgaggagg tgcgcgctgg ggccaacgcc cggttagcgc gcgctgatct
     1621 gtttcatggc cacatcaact tctacaaagg cacgttgctg atcgtttttc atgctgccga
     1681 gtatcccatc gaccttctgg acaccaaggc tccagccaaa aacgcagcac tcgtcgagcc
     1741 gggggtcacg atcaaggcga ccgatgcaaa ctttcgctac cgcaacgcga tctactgctt
     1801 ttccacaggc attctggaga cgctcgacac tcaaaaactg gacgcgaagt ggaaagacaa
     1861 tttcgatcca aagagcatca ctctgctgga tgacacattg ggctcgtcag tcgccgggct
     1921 gaacttgttc tcaaccgagg gcattccctt tccattcctg gcctgacccg gtcagcgcgg
     1981 gtctgcacga tataagaact accctgcgtc aacggtcgca cgtgtcgtca ccgatcttga
     2041 cacgggtctt cgccgcgaat ctgtggcaag acctaagcgt gccagtgacc cacgacaaac
     2101 cccacagccc gctgagtttg cttgagatcg gtgcgatcct tgtgatcatc gtcacctggg
     2161 gcgtgaataa cgccgcaggc aagctggcca ccgagaccat gcctcccctg atggtcggag
     2221 cgctgcgttt cgccatcgcc accatctgcc tcgccgggtt cgtgcgcccg ccattcccgg
     2281 actggaaaag cctcgttttg atcgtggtga tcggcggggc ggtgcagtac gggctgcttt
     2341 ataccgccta ttggctggcg caggacgtca gccctgcgac cgtggcgaac cagctgtgga
     2401 tccccatgac ctcgctgttc gccttcctgc tgctcaagga gaagctcagc cgcgcggcga
     2461 tggtcggcat ggcgatagcc ttcgccggtg tcggctggat gatgctggac agccatgcgc
     2521 tgcaggactg gcgcgccatt ctcgtcgcca tcgcggcggg tgcggcctgg gccctgacca
     2581 cggtcgtggc gcgccgcacg acctccattc caccgctgaa gatgcaaggc ctgctggctg
     2641 ccggcacact gccgatcatg gccttcgggt cggcggtgtt tgagcatggt cagatcgagg
     2701 cggtccgaca cgccacgccg ctggtctggg cgtcactgtt ctgggcaggg gtcatatcgt
     2761 cggtgttcgc caccaccctg ctgttctggc tggttcagaa gcgtgaggcc gggcgggtga
     2821 cgccttatct gctggcgacg cccgtggtga ccatcctgat cggctggttg ttcatgggcg
     2881 atgtgctgac accgcaaatc ctgaccggcg ccgccctcgc gatgggcggc gtcgcggtcg
     2941 tggccctggc cgagcgaggc ttgcgcgcag gcgcggccaa ggcctgatcg ggtcttagtg
     3001 gcccgtgtgt gccgcggact ggaagacgcc gcacgcgatg cgggcgcccg ctccaccgat
     3061 cggctgcgag acgtaatcgt cggggctggc gtgcacgagg aacgacaggc cgttggcgtc
     3121 gatatagtcc gaaggtttcg cgttcaggcc ttcccaagcc agataaacgt cggcgtgaac
     3181 cgagccgtca gcagcggcgt agacgttctg cagatcaccg aggtctgggc cgccgctctt
     3241 gttcagcagg ccatgcgggc gagcgccctc ggcgtggttc acgtgaccac cggctgaggt
     3301 gaaggccgga ccttcgcagg ttccgacggc atgcaggtga acggcgtgcc agcctgctgg
     3361 ccagcccttc ccgtcgatgc ggaagatcat gcccttcggg ccttcgaatg acgtgacagt
     3421 gcccgcgtcg gcaccgtctg cggtcttcag agccgcggta gctacggcaa ccggaacggg
     3481 cgctggagca ggtgtgggcg cggctgcagg ggcggccgga gtggtctggg cgagggcggc
     3541 tccggcgccg agaatagaac tggcggcgag agcagcgaac aaggggaggg cgcgcatgga
     3601 atcttccttt cacggacgac gtcgaaacgg gcgcaaactt tgccaccgga cgccacggat
     3661 gctagaaaac cggacagcgg atcacgttcc gattccggcc acttaaagcc taaacgcctt
     3721 gaccgacgac agctacgaaa acgccgcacc tcccgccaat tccggcggtt ctggatcggg
     3781 cggtggttcc gacatttcca cgatcacgat cgaggacgag ctgaaacgct catacctcga
     3841 ctatgcgatg agcgtgatcg tttcgcgcgc cctgccggac gcccgcgatg gcctgaaacc
     3901 ggttcaccgt cgcatcctgt attcgatgca cgacctgaac atgacgccgg accggtcgta
     3961 ttcgaagtgc gcgcgcgtcg tcggtgacgt gctcggtagg ttccacccgc acggcgacgc
     4021 ctcggtctat atggccttgg tgcgcatggc ccagccgttc tcaatggggc tgatgctggt
     4081 cgatggtcag ggcaactttg gctcggtcga tggcgacatg cctgcctcga tgcggtacac
     4141 cgaagcccgg atggctccgg cggctgtggc gctgctggcc gatatcgaca aggacaccgt
     4201 cgatttccag ccgaactacg acgagaagga acttgagcct gtcgtcctgc cggcccggat
     4261 cccgaacctg ctggtcaatg gcgcaggcgg catcgccgtc ggcatggcca caaacatccc
     4321 acctcataat ctcggcgaga tcgtcgatgc ggccgtggcc ctgctcgata acgccgacat
     4381 cagcgacgac gccctgctgg acatcgtgcc cggtcccgac ttcccaaccg gcggcgagat
     4441 catgggtcgc acggccccgc gtaacgccct tcgcgacggt cgtggttcag tcatcgtgcg
     4501 cggcaaggcc tcgatcgaga ccatccgcaa ggaccgcgaa gccatcgtcg tcaccgagct
     4561 gccgttccag gtcaacaaac agaccctgat cgagcgtatc gccgagatgg tccgtgagaa
     4621 gcggatcgaa ggcatctccg acgttcgcga cgaatccgac cgtcagggca tgcggatggt
     4681 gatcgagctg aagcgcgacg catccggcga tgtgatcttg aaccagctgt ggcgctacac
     4741 ggccatgcag tcgtcgttcg gcgtcaacat gctggccctg aaccacggtc gtcccgagca
     4801 gatgggtctg cgcgacctgc tgacaatctt cctcgacttc cgcgaagaag tggtcgttcg
     4861 ccgtgtgaaa ttcgaactgg ccaaggcccg tgaccgcggt cacgtcctgg tcggtctcgc
     4921 cgtcgccgtc gccaacatcg acgaagtcat ccacatcatc cgctcgtccg ccgatcccgc
     4981 agaggcgcgc cagcgcctgc aggccaagcc gtggcccgtc gccgacatga tcgccttggt
     5041 cgagctgatc gccgacccgc gctcgatgct gatcgacggt gacaagctga acctgacgga
     5101 cgaacaagcc cgcgccatcc tcgccctgac cctgtcccgt ctgaccggcc tcggccgcga
     5161 cgacatcttc ggcgaggctc gtggtctggc cgacaccatc cagggccacc tgaccatcct
     5221 gtcggaccgc gccaacattc tcgccatcat ccgcgaggac ctgctgctgg tgaagaccga
     5281 gttcgccgtg ccgcgccgca cgacaatcgg cgaaggcgac gccgagatgg aagacgaaga
     5341 cctgatcccg cgcgaggaca tggtcgtcac cgtcacccac ggcggctatg tcaagcgcgt
     5401 ggccctgaac gcctatcgga cccagcatcg cggcggcaag ggcaagtcgg gtatgacgat
     5461 gaaggacgag gacgccatca ccggcgtctt ctccgcttcg acccacacgc cggtgctgtt
     5521 cttcgccacc aacggcaagg cctacaagct caaggtttgg cgcctgccgc tgggcggacc
     5581 gacgtcgcgc ggcaaggcct tcgtcaatct gctgcccatc gagccgggcg acagcatcat
     5641 gaacgtgctg ccgcttcccg aggacgagac gacctggggc gactacgaca tcatgttcgc
     5701 caccaagtca ggcgacgtgc gtcgcaacaa gctatccgac ttcgcgtcgg tcaaccgcgc
     5761 aggcaagatc gcgatgaagc tggaaggcga tgaccatatg gtcggcgtcg ccctgtgcac
     5821 cgccgaggac gacgtcatgc tgacgacggc gctcggccgc gccatccgct tcaaggccga
     5881 cgacgttcgc gtcttcaagg gccgggattc gaccggcgtt cgcggcgtcc gcctgcagtc
     5941 cggcgacgag gtcatctcga tggccgtgct tggccgggtc gatgcgagtc cagaagaacg
     6001 caccgcatac gtcaaacacg ccaacgccat gcgcaaggcg atgggggagg gcgacgacga
     6061 aacggctgtg gtcgaggctg atgaagacgg cgcagaggag ggcggtgaag ccgtcctgtc
     6121 cgtcgagcgc atcgcggcat tgggcgcggc ggagcagttc attctgaccg tcaccgacac
     6181 cggcttcggc aaacgatcct cggcctacga gtatcgtcgc accggtcgcg gcgggcaggg
     6241 gctgacggct cacggccttg gcggccgcgc cggcaaacgt ctcgccgccg ccttcccggt
     6301 cgaggacacc gacgaactgc tgatggtcac ctcgaccggc cagatgatcc gcacccgggt
     6361 cggtcaggtc cgcgtcgtcg gccgctccag ccagggcgtt accatcttcc gcactgctga
     6421 aggcgaacgc gtagtctcgg tcgaacgtct ggccgaaacg ccgggtggcg atgaggatct
     6481 cgatttggac accggtggag acgacggcgt cgaagggacc gaggacggcg gcgaaaactg
     6541 atcccatgac tggttttccg ccgattttgg tcgacggaga gccgatctcg accaacgatc
     6601 tgatctacct cgcgctcgtc aactacgccg cctacacctc gttcagggtg gaggcaggcg
     6661 cggcgcgggg gttgtcccgg cacctcgacc ggctccacgc ctcggcgttg gagctgttcg
     6721 gggaaagtcc cggcaaggcg cggtttcggg acctgatgcg caccgctgtt gaagggcacg
     6781 aggcctgctg gcttcgtgtc agcctgttct cgcccgaact gtgggcgagg acgccgtctt
     6841 ggcgcgggat gccgaaggtc atgaccatgg ccgcccagcc gccaccgccg ttggccgcct
     6901 cgctgaggct ggcgacgagg gtctactcgc gggatgcggc gcatctgaag cacaccgcag
     6961 tgttcgggct aatccagtcc cggcgacagg cgcaggatga gggcttcgac gacgccctgt
     7021 ttgtcgataa ggcggggctc atctcggagg gaagcctatg gaacgtcggc tttgtgcgcg
     7081 gtgaccgcgt gacctggcct caggcgccga tgctgtcggg tgtgacgcag gggctgatcg
     7141 agcagggatt ggccgacgtc ggactaacgg cggaaacccg tccggtaaaa ctgtcagacc
     7201 tgccggattt cgatcacgcc ttcatctgca acagcgcaac gccggcttgc gctgtgacag
     7261 ccattgacgg tcatggcttt gaaagcgatc cagcgttgat cgagcgtctt catgcggctt
     7321 ggtcgtttaa ccggcctgaa gcgatctaga cctacggccc gacgtcgttg gcgactgcgg
     7381 cgccgtcgtc gatggggacg ggcaggctga cggcgatgcc caaggcttcg gccagcttct
     7441 ggtcgcgctt gtagacgtcc tcgcggaagg cgacccgttg ttggccgtcg acgaaggcgg
     7501 tccagtagag caggcgaacg gtgatctcgc ggcccgtctg aatgcgctgg gtatcgcgcg
     7561 tgatctgggc cgtgtcgaac tctgacagac gcacgggatc aggcgacagc agcagccggg
     7621 cgaactcgac cgcaccctga acacgaacgc agccatgcga gcgctggcgg gtggccagat
     7681 cgaaggcgcc cttggacggg gtgtcgtgca ggaagatggc gtagctgtcg cgcagctcga
     7741 attttacata gccgagcgcg gcctgtggtc cggcacgctg gatgacctga ccgttggaga
     7801 catacatgtc gcgtgcagcc atataggctg ggccctttgg caggatctcg cgacgggcga
     7861 taccggcagg gacataccag ggtgggttgg ccacgaccga ggcgaatggt ttctcgaggc
     7921 tgggtgtctg gttttcggct gagccgacga cgacgcggtt ggagtgaacc gggcggccgt
     7981 ccttccagta gaccatgatg gcggcggccg tgttgacctc gatgcgctcg gggctgagct
     8041 cgcgcttcag ccagcgacga cgttcgaggt tcagcgcgat ctggcgcgcg cgatcgtcgg
     8101 atgaggcgcc caatgaccgt tgcgtacccg cgcccagacg ggcgtcggcc aacaggccgt
     8161 gacgttgctg gaagccgcga accgccgtct gcagttcgtc gccgtaggtc aaaccctgag
     8221 ccttcaggcg agcgccatcg gcggcgctga catcaccctc ggccaccaga cgatcgatca
     8281 gcaccgggat gcgacgatcg ctcatgcccg gttcgatgct ggcgccttgg gtgaacgtcg
     8341 gccaaccgcc cgcacggctg agacggcgat agcgaagata gccgttcgac aggttggtat
     8401 aaccaatatc cgttggcggc agtccgtcga accactgcac caactggttc tgggcaaggg
     8461 cattgttcag gcccgtcggc agatccacgc ggttcttgtc catctcccac aggtcttcga
     8521 catcctcggg ccggacgcgg ccttcggcaa gaacctgggc atacgagata gcggcggcgg
     8581 tggtgcggac ctccgcgctg gccggatcgg cggcgaggcc gacgaagtcg aagaaatcgg
     8641 tcgcgggcag accgtggttt tccgacttcc gggccacggc attgagggct tgtacctgtt
     8701 gcggagccca gaccgtcttc cagccgttca tctcgtagaa tttacggacg tcatcctgaa
     8761 gagtggcggg cagacgcgcg atctgcggag cgtagacgcc ctggccgccg cgcgtcgccg
     8821 cgactgcggg tatggcgacg cgaggctgcg agcggcgata ctgggcggcc tgcgcggacc
     8881 ctgctgcaga agcgatcgca gcggttgcga tgccgaagct taattcccgt cgattcatcg
     8941 tcgtccgctc ttgatccgtt cgtctcgctc gtaagcgaag aacgagtcga tgttaccggg
     9001 cttccccgta aacaaaaagc gccggtctga tgaccggcgc ctttcgtgat cgtaaaagcg
     9061 cagtcgctgc gctcgaaacg accttacttc ttgatgaagc cgccaaactt gttgttgaag
     9121 cgcgagacgc ggccgccacg gtccagcatc tgctggttcc cgccggtcca ggcagggtgg
     9181 gtcgacggat cgatgtccag gttcaggacg tcgccctctt tgccgtaggt cgagcgcgtt
     9241 tggtacgtgt cgccattcgt cagggtgacg gtgatgaagt ggtagt
//
LOCUS       DASVTM010000427         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_410556,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000427 DASVTM010000000
VERSION     DASVTM010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410556"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..931)
                     /gene="xth"
                     /locus_tag="VF633_11800"
     CDS             complement(113..931)
                     /gene="xth"
                     /locus_tag="VF633_11800"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8471786.1"
                     /translation="MRIATWNVNSVNARLPTVIAWLEAAMPDVVCFQEIKTIDEKFPR
                     EEFERLGYNIETHGQKTYNGVAILSKYPLSDVRRGLPGAEGAGIDGADDDHARYIEAV
                     VEGPVPVRVGAIYLPNGNPVATGKFPYKLAWVERLNAHARDLLSLEEPFVLCGDYNVI
                     PQPEDAKNPANWIGDALFQPESRAAFQALKWLGLSEAGELGEQPPGTYTFWDYQAGAW
                     QRDNGIRIDFHLLSPQAADLFRGVETHRDARDMDKPSDHVPVVVELSDIPTLAA"
     gene            complement(1056..2102)
                     /locus_tag="VF633_11805"
     CDS             complement(1056..2102)
                     /locus_tag="VF633_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471787.1"
                     /translation="MFKKILAMAAAMVLTAGAPVLAQTATPPVVNPGNTDNNGTQVNG
                     MDRTITGERVTAGRIQGRQRARDRAAEQAEGARAQQPARGRGDSAAPNLEQVKAAAQA
                     LATATNSGCTIAEATLRGESPEKQSVYEATCTSGPGFILIGSTPPQAVDCVILSGQAD
                     ISRSRDPAADVGLQCEMEPNKNVLAVVTGYATQAGVACTVDQGSSVGKSAEGNVIYEI
                     GCTGTDGYWLEKTATGWTTTECMKLMSRSATCKYTTPVEQAATLKKWFEGSAAAPCDV
                     AQARFMGSNANGSFYEAKCVAGDGLIVRFDTAMAVQQVYPCAEAQRIGGGCTLTVVAL
                     APATAPAAAPTTQQ"
     gene            2239..>3041
                     /locus_tag="VF633_11810"
     CDS             2239..>3041
                     /locus_tag="VF633_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8471788.1"
                     /translation="MADGVIPMEVASGPRVERGLTYPLGSPPAPGEAVEAAPGVLWLR
                     LPLPMSLDHINVYALADGDGWTLIDTGLNTKVSIAGWEAAFIGPLGGKPVKRVICTHM
                     HPDHLGLAGWLCARFGVPLLMSRLEYVTARMLVADTGPAPREGEIFFRAAGWDDEQID
                     VWRSQFGRFGKGVYPMPQSYHRLSEGDDIEINGDIWSIVVGNGHSPEHVCLWRQSDGV
                     FLAGDQILPRISSNVSVWPTEPMQDPLGDWLTSLEKLRALLPAETFVLPS"
BASE COUNT          490 a          920 c         1031 g          600 t
ORIGIN      
        1 gcgtcgaagc cgccgctcag gctccatcca ccagttgagc gtcaaggcgc cggtggtcag
       61 ggccgccgct gcgagaccga ccagcaggat gaccctcagc gcttcggtca cgtcaggcgg
      121 ccaaggtcgg aatgtccgac aactcgacca cgacgggaac gtggtccgaa ggcttgtcca
      181 tgtcgcgcgc gtcgcgatgg gtttccacgc cacggaacag gtcggcagcc tgtggcgaga
      241 gcaggtggaa gtcgatgcgg atgccattgt cacgctgcca ggcgccggcc tgatagtccc
      301 agaaggtgta ggtcccggga ggctgctcgc ccagttcgcc ggcctcggac agacccagcc
      361 atttgagcgc ctgaaaggcc gcacggcttt ctggctggaa cagggcgtcg ccgatccagt
      421 ttgcggggtt tttggcgtct tcgggctggg gtatgacgtt gtagtcgccg cacagaacga
      481 agggttcttc aagcgacagc aagtcgcgag cgtgggcgtt cagccgctcg acccacgcaa
      541 gcttgtaggg gaatttgccg gtcgcaaccg gattgccgtt cgggaggtag atcgcgccca
      601 cccgcaccgg gaccgggccc tcgaccaccg cctcgatata gcgggcatga tcgtcatccg
      661 cgccgtctat gcctgcgccc tcggctccgg gaagtccgcg ccgcacgtct gacagagggt
      721 atttcgacag gatggccacg ccgttgtagg tcttctggcc gtgggtctcg atattgtagc
      781 ccagacgttc gaactcctcg cgagggaatt tctcgtcgat cgtcttgatc tcctggaagc
      841 agacaacgtc gggcatagcc gcctcgagcc aggcgatgac cgtcggcagg cgggcgttga
      901 cggaattgac gttccaggtg gcgatgcgca tgcgccggac gctagtgcgt ggccgggtaa
      961 agggaaagcc ggtttgcggt cgtgaggtcg gaccgcaaac aaaagagccg ccccgggaac
     1021 gggacggctc tcgaagagta ggttcgagcc tgagactatt gctgcgtcgt aggtgcagcc
     1081 gccggtgcgg ttgccggggc taatgcaacg acggtcaggg tgcagccgcc gccgatgcgc
     1141 tgggcctcgg cgcagggata aacctgctgc acggccatgg cggtgtcgaa gcgcacgatc
     1201 aggccgtcgc ctgctacgca tttggcttcg tagaaagagc cattggcgtt ggagcccatg
     1261 aagcgggctt gagcgacatc gcaaggcgcg gcggcgctgc cttcaaacca cttcttgagc
     1321 gtcgcggcct gctcgacagg cgtcgtgtat ttgcaggtgg cgctgcggct cattagcttc
     1381 atgcattcgg tggtggtcca gccggttgcc gtcttctcga gccagtagcc gtccgtaccg
     1441 gtgcagccga tctcatagat gacattgcct tcggccgact tgccgaccga cgatccttga
     1501 tcaaccgtgc aggccactcc ggcttgcgtg gcgtagcccg taacgaccgc taggacgttc
     1561 ttgtttggct ccatttcgca ctgcaggccg acatcggcgg cagggtcacg agagcgcgag
     1621 atatccgctt gtcccgacag gatcacgcaa tccacggctt gcgggggcgt cgaaccgatc
     1681 aggatgaagc cgggcccgga ggtgcaggtg gcctcgtaga ccgactgctt ttcagggctt
     1741 tccccacgca gcgtggcttc ggcgatcgtg cagccgctgt tggtggcggt cgccagcgcc
     1801 tgtgcggctg ccttgacctg ctccaggttg ggtgcggcgg aatcaccacg tccgcgcgcc
     1861 ggctgctggg cgcgcgctcc ttcagcttgt tccgcagcgc ggtcgcgcgc gcgctggcgg
     1921 ccctggattc ggcctgcagt aacgcgttca ccggtgatgg tgcggtccat gccattaacc
     1981 tgtgtcccgt tgttgtcagt gtttccggga ttgacgacgg gcggggtggc ggtctgcgcc
     2041 agcacgggag ctcccgccgt aaggaccatg gcggcggcca tggctaggat tttcttgaac
     2101 acgcgacggc tccgggcaac tgaaggatca ggttcttatc ctttgcgtcg gtctgcagcc
     2161 gggtcaagct attcccggtc ggcactatgg acgtcgaagc tcgcttgcga ctaaagatcg
     2221 tttcgatagg agactgactt ggctgatggc gtgatcccga tggaggtggc gtccggaccg
     2281 cgcgtcgaac gcggtctgac gtatccgctc ggctcgcctc cggctccggg tgaggctgtc
     2341 gaggccgcgc cgggcgtgct gtggctgcgg ctgcccctgc cgatgtcgct ggatcacatc
     2401 aatgtctatg cgctggctga cggcgacgga tggacgctga tcgacaccgg tctgaacacc
     2461 aaggtctcca tcgccggctg ggaagccgcg ttcatcggtc cgttgggcgg caagccggtc
     2521 aagcgggtga tctgcaccca catgcatcca gaccatctcg ggctggcagg ttggctttgt
     2581 gcgcggttcg gcgtgccctt gctgatgtca cggctcgagt acgtcacggc ccgcatgctg
     2641 gtcgccgata ctggccctgc gcctcgcgag ggcgaaatct tcttccgcgc cgcaggttgg
     2701 gacgacgagc agatcgatgt ctggcggagt cagttcggac gcttcggcaa gggcgtctat
     2761 ccgatgcctc agagctatca tcggctcagc gaaggcgacg atattgagat caatggcgac
     2821 atctggagca tcgtcgtcgg caatggccat agccccgaac acgtctgcct gtggcgacag
     2881 tctgacggcg tgtttctggc cggtgatcag atccttccgc gcatctcatc gaacgtctcg
     2941 gtctggccta cggagccgat gcaggatccg ctgggggact ggctgacgtc gctggaaaag
     3001 ctgcgtgcgt tgttgcccgc agagactttc gtcctgccgt c
//
LOCUS       DASVTM010000428         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_734337,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000428 DASVTM010000000
VERSION     DASVTM010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734337"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VF633_11815"
     CDS             <1..1060
                     /locus_tag="VF633_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8471789.1"
                     /translation="PTGAQLPEIDTYDVGLDASYEIDLFGRVGSSIRAARADADAQTE
                     AVDTIRVSVAAETARAYADACSANAQIAVAERTLGLQQNTADLTQRLLEGGAGTGLDT
                     SRAQVALAQTRATIPTLRAARDEALFRLSTLTGRTPAEVSEAARSCARPPQLSQPIPV
                     GDGAALLARRPDVRQAERQIAAAAARVNVATASLYPSISLGGSFGSTALDSDDLGKDE
                     NYRFSFGPLISWSFPNIAVARARIAQTSALTDAALANFDQTVLAALQETETSLTNYAN
                     ELDRRTALTAARDQAATAARLSRLRFEAGADNFLIVLDAERTLAGADAQLAQSDALVT
                     TYQIALFKALAGGWQAEA"
     gene            complement(1154..>1946)
                     /locus_tag="VF633_11820"
     CDS             complement(1154..>1946)
                     /locus_tag="VF633_11820"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8471790.1"
                     /translation="AWFRPDNATLVVVGDVTMAELQPQLERAFVGWRAPSTPLPAKPE
                     ATVATAPAQPQVFLVNRPGPQSIIMAGRLTAPFDAATEPGLDTFNSALGGTFTSRINM
                     NLREDKHWSYGASSGVRTANGPRLFAATAAVQSDKTAESTAEVRKELAEVISQRPMTQ
                     AEIDATKANLVQGMAGEWETNGAVMNDLTQMVVYGRPENYFDTYAERVRATTPQSASL
                     AAQSVVGTGPTTWVIVGDVATMKPKIEALNLGQVQVVDVNGNPVP"
BASE COUNT          310 a          697 c          623 g          316 t
ORIGIN      
        1 cccgactggc gcgcaactgc ccgagatcga cacctatgac gtcggtctgg acgcgtccta
       61 cgagatcgat ctgttcggtc gcgtcggatc ctccatccgc gccgcccggg ccgacgccga
      121 cgctcagacc gaagctgtcg acacgatccg cgtcagcgtc gccgccgaga ccgcccgcgc
      181 ctatgccgac gcctgttccg ccaacgccca gatcgccgtc gccgaacgga ccttgggcct
      241 gcagcagaac accgccgacc tgacccagcg cctgctggaa ggcggcgcag gcaccggtct
      301 cgacacctcg cgcgcccagg tcgcactggc ccagacccgc gctacgatcc cgacgttgcg
      361 cgccgcacgt gacgaggccc tgttccgtct gtcgaccctc acaggtcgga caccggcaga
      421 agtctccgaa gccgcccgca gctgcgcccg tccgccgcaa ctgtcgcagc cgatcccggt
      481 cggcgacggc gccgcgcttc tggcccgccg tcccgacgtg cgtcaggccg aacgtcagat
      541 cgctgctgcg gccgctcgcg tcaacgtcgc cacggcgtcg ctgtacccga gcatcagcct
      601 tggcggatcg ttcggctcca cggcgttgga ctcagacgat ctgggcaagg acgagaacta
      661 ccggttctcg tttggcccgc tgatcagctg gtcgttcccc aacatcgcgg tcgcgcgcgc
      721 ccggatcgcc cagaccagcg ccctgaccga tgcggctctg gccaatttcg accagacggt
      781 gctggccgcg ctgcaggaga cggagacgtc tctgaccaac tacgccaacg aactggaccg
      841 tcgcacggcc ttgaccgctg cgcgggatca ggccgcaacc gccgctcgcc tgtcacgcct
      901 acgcttcgaa gccggcgcgg ataacttcct gatcgtgctc gacgcagagc gcacactggc
      961 cggcgccgac gctcagttgg cccagtccga cgctctggtc accacctacc agatcgccct
     1021 gttcaaggcg ctggccggcg gctggcaagc ggaagcctaa gtcctcccct caaggggata
     1081 cagttagaca agcaaaaggg gccggtgatc actcaccggc cccttcgccg ttctggcggc
     1141 ggtcgccggc tccttaagga accgggttgc cgttgacgtc cacgacctga acctgaccga
     1201 ggttcagcgc ctcgatcttc ggcttcatcg tggccacgtc gccgacgatg acccaggtcg
     1261 tcggacccgt accgaccacg gactgagccg cgagcgaagc cgactgcgga gtcgttgcgc
     1321 ggacccgttc ggcgtaggtg tcgaagtagt tctcgggacg gccatagacg accatctggg
     1381 taaggtcgtt catgaccgcg ccgttggttt cccactcgcc agccatgccc tgaacgaggt
     1441 tggccttggt cgcgtcgatc tcggcctggg tcatcggccg ctggctgatg acctccgcca
     1501 gttccttgcg gacttcggcg gtgctctcgg cggtcttgtc ggattgaacc gccgccgtcg
     1561 ccgcgaacag gcgaggcccg ttggcggtgc gaacacctga gctggcgccg taggaccagt
     1621 gcttgtcctc acgcaggttc atgttgatgc ggctggtgaa ggtgccgccc aatgccgagt
     1681 tgaacgtgtc cagacccggc tcggtcgcgg cgtcgaatgg cgcagtcagg cgaccggcca
     1741 tgatgatcga ctgcggaccc gggcggttga ccaggaacac ctgcggctgg gccggagccg
     1801 tagccacagt ggcttccggc ttggccggca gaggcgtgga cggcgcgcgc caaccgacga
     1861 aggcccgttc cagctgaggc tgcagctcgg ccatggtcac gtcgccgaca accaccagcg
     1921 tggcgttgtc gggccggaac caggca
//
LOCUS       DASVTM010000429         5091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_146046,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000429 DASVTM010000000
VERSION     DASVTM010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146046"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VF633_11825"
     CDS             <1..951
                     /locus_tag="VF633_11825"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8471791.1"
                     /translation="LRRADYQDNGSENFTNYQVGGTGQVDVRRDTSIGFGADYGKFTE
                     LRTSNNAPTNVAEPVEYDQTGANLQLVHSFNRLRLLGRIDRVELDYQNGVDALGGQVF
                     QDDRDRSVTAVTGRSEYAVSPDTAVFFEAVFNTREYDLSPPDVVTDRDSDGSEISLGA
                     NFDISRLARGEVQLGYLTQDYTAADLEDFANLTGRARLEWFPSDLLTVSFVTGRTVED
                     ATGAGISGYLSTTAGLTADYEVRRNLVLSAGATYEAADYGDIDRDDNTWTSQIEATYK
                     VNRTLAISAGFEHLERDSSGLSATTDYRINRFQLAFVLQR"
     gene            978..3170
                     /locus_tag="VF633_11830"
     CDS             978..3170
                     /locus_tag="VF633_11830"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX8471792.1"
                     /translation="MNTMTPPALQSADSAGGGFDPNRLIAAFRRRLRLFAVVVAVIIG
                     AAVVSILLATPRYTASSDILLDTKPTPVVQDSLALPETPTAANAVDTEVEVLRSRALA
                     EKVVTGLRLDQIPEFAGGGPGLLNAFRPAAAADTSRAAPPTGNARQKIVDTVMSRLSI
                     RRAGLTDVINVGFESEDPKLAAKVANAFAEQYLTDQLDAKFTANRQANEWLQRRVGEL
                     AAEVQTAEVAVQQYKIANNLMSAQGATLVEQEISSFEQQRAAAATEQAAADARLAIAR
                     QQLARGSNGQDVGEALDSPVIQQLRQQRATLSARVADLSGRYGPRHPEMLRADRELRD
                     IDGQINAEVQRIISNLDAQARVARQRTGVIAGSVGNARQNLAGNNRAGVRLNELERSA
                     QSVRVLYESYLNRYKETSAQQGLEHSDARIVSAASVPTDASYPNKILQLGLGVLLGIG
                     AGLAMVILAEALDNGVRTSDDVERLFDLRSLGAIPELKSTFEPGVRSRAGPTDFLVDK
                     PMSAFAESFRSLRTSIAFSGSGGPVQVVVVTSSLPGEGKTTSAMCLARSAALGGLKAI
                     IVDCDLRQRAVRRYIEQDINVGLVEVLSGSATLAQAVIKDSRTEAMILPIAGDVGVAR
                     DLFGGAAIEKLIAELRQHFDLIVLDTAPVLPIADTRMIAHQADAVIVLARWSKTPRKA
                     IESSLRLLASTGAFIGGVVLTKVDMKAQARTGYGDSGYYYKDYKAYYG"
     gene            3163..>5091
                     /locus_tag="VF633_11835"
     CDS             3163..>5091
                     /locus_tag="VF633_11835"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8471793.1"
                     /translation="MAETLTAWRGPPGDFRWRPWLHAGRGLSARELELIWAVLAQIFI
                     AHLLFGANRNDLSLAFALVQAALVLRALTAPWARQALRPGRLLLPGILYAIVLVFLVI
                     SLIPGENPRLFAAFPWLVSPTGSINTEASLIALCRMGGLACVFIQGILIGASRSRAAF
                     FMNAFVAAATLYAGVAILGFWIAPDTLYGVDRPYHSDRLAGTFLSANSAGAFFGMALV
                     ASFGLLFRRGRSSRVSGRRIAVSVSVALLAIALLLTASRGALAATLAASAFLMLGRAA
                     MARRTGWVRSSLMAATGAAIIVVAAVLLSPISGRLSAAATDIDVRAAIINAHWPAIRS
                     ELWQGYGAGAFERVNAMVQTPSTVDRLWSVRALHNVYLQWLEEAGLVGTLAMWGVIAV
                     VLGGITSHLLGRRPSRWPPVVIAMSVMILLHGFVDYGLEVPSLSAQWALFLGLGYAST
                     MPRLRGQNKRGGIKPGFSLSRRPRLRLGGVAICASATLLLAGGILATGIVRQNRATEV
                     QSLDALREATRRSPADGQAWLIAAVAERNAAKRCARLCVTDLSNSYNAAPLSRTIFAW
                     RTRFVLEHWDQVPVTLRVSALRHIHAQWRTRSGRRDIEGMSRVVFNPAGRLAIKIAAN
                     GTAPGDSSAAVEAAEQGPN"
BASE COUNT          875 a         1647 c         1597 g          972 t
ORIGIN      
        1 ctgcgacgtg ccgactacca ggacaatggt tccgagaact tcaccaacta tcaggtcggc
       61 ggcaccggcc aggtcgacgt acgacgtgat acgtcaatcg gtttcggggc cgactacggc
      121 aagttcacgg agctgcggac atcaaacaac gcgccgacga atgtcgcaga gcccgtcgaa
      181 tacgaccaga ccggcgcaaa ccttcagctc gtccatagct tcaacaggtt gcgactgctg
      241 ggccggatag accgtgtcga gcttgactac cagaatggcg tcgacgccct gggggggcag
      301 gtcttccagg acgaccgcga ccgcagcgtc accgccgtaa ccggacgcag cgaatatgca
      361 gtcagtccgg atacggcggt gttcttcgag gcggtgttca acacgcggga atacgacctg
      421 tcgccgcccg atgtcgttac ggatcgcgac tcagacgggt ccgagatctc cctcggggcg
      481 aatttcgaca tcagccgtct cgcccggggc gaggtccagc tgggctatct cacgcaggat
      541 tacaccgcag ccgacctgga ggatttcgcg aacctgaccg gccgcgcccg tctggagtgg
      601 tttccaagcg atcttctgac ggtcagcttc gtcaccgggc gcaccgttga ggacgccacc
      661 ggcgcaggta tctcgggcta cctctccacg actgcggggc tgacggctga ttatgaggtc
      721 cgacgcaacc tcgtgctgtc tgcgggtgcg acgtatgagg cggccgatta tggcgacatc
      781 gatcgtgatg acaacacctg gacctcccag atcgaggcga cctacaaggt caatcgcacc
      841 ctcgcgatca gtgcgggctt tgaacatctg gaacgcgact cctcgggtct gtcggcaacg
      901 acagactacc ggatcaaccg cttccagctg gccttcgtct tgcagcgctg accgcaacaa
      961 tttcaagagt gcctcaaatg aacacgatga ctccaccggc cctgcagtcg gcggacagcg
     1021 caggcggcgg ctttgacccc aacaggttga tcgccgcctt ccgtcggcga ctccgccttt
     1081 tcgcggtggt cgtcgcagtc atcatcggag ctgcagtcgt ttcgatcctg ctggccacgc
     1141 cgcgatatac cgcgtcctcg gatatcctgc tggacaccaa accgacgcct gtggtgcagg
     1201 attcgctggc cctgccggaa acgccgaccg ccgccaatgc cgttgatacc gaagtggagg
     1261 ttctccgctc ccgcgccctg gccgagaaag tcgtcactgg cctccgcctc gatcagatcc
     1321 ccgagtttgc aggcggtggc cccggtttgc tgaatgcttt ccggcccgcc gcggcggcag
     1381 ataccagccg ggcggctcct ccgaccggca acgcgcgaca gaaaatcgtt gataccgtca
     1441 tgtcgcggct cagcatccgc cgggcaggcc tgaccgacgt gatcaatgta ggcttcgagt
     1501 ccgaagaccc caagcttgcg gcaaaggtag ccaacgcctt tgcggaacag tatctcacgg
     1561 atcagctgga tgccaaattc accgccaatc gccaggcgaa cgagtggctt cagcgtcgtg
     1621 tcggcgagct tgcggccgag gtccagacgg ccgaggtcgc ggtccagcag tacaagatcg
     1681 ccaataatct gatgagcgcg cagggcgcca cccttgtcga acaggaaatt tcctcgtttg
     1741 agcagcaacg ggccgcggcg gccaccgagc aggcggctgc cgacgcgcgt cttgcgatcg
     1801 cccgtcagca actggcgcgc ggcagtaacg ggcaggatgt cggcgaggcg ctcgattcgc
     1861 cggtgattca acagctgcgg caacagcgcg cgaccctgag cgctcgcgtt gccgacctgt
     1921 cgggccgcta cggcccgcga cacccggaaa tgctgcgcgc agaccgcgaa cttcgcgata
     1981 tcgacgggca gatcaacgcc gaggtccagc gcatcatctc caatcttgat gcgcaggccc
     2041 gcgtcgcccg tcaacggaca ggggtcatcg ccgggagcgt cggcaacgcc cgtcagaacc
     2101 tggccggcaa caaccgggct ggcgtccggc taaacgagct cgagcggtcg gcccagtccg
     2161 tccgggtgct ctatgagagc tatctgaacc gctacaagga aaccagcgcc cagcaggggc
     2221 tggagcactc cgatgcccgt atcgtttcgg ctgcaagcgt gccaaccgac gccagctatc
     2281 ccaacaagat tctccagctt ggactgggcg tccttctcgg gatcggggcc ggtctggcta
     2341 tggtcatcct ggccgaggcc ctcgacaacg gcgttcgaac gtctgacgac gtggagcggc
     2401 tcttcgacct gcgctccctt ggcgctattc ccgagctgaa atccacgttc gagcccggag
     2461 tccggtcacg ggctggaccg acagacttcc tcgtcgacaa acccatgtcg gccttcgcgg
     2521 aatcgttccg cagtctgcga acctcgatcg cgttctcggg cagcggcggt ccggtgcagg
     2581 ttgttgtggt tacgtcatcg cttcccggtg aaggaaagac gaccagcgcg atgtgtctgg
     2641 cgcggtcggc ggcgctcggc ggcctgaagg cgatcatcgt cgattgcgac ctgcgccaac
     2701 gggccgttcg tcgatacatc gaacaagaca tcaacgtcgg cttggtggag gttctttcgg
     2761 gctccgccac cctggcgcag gctgtgatca aggacagccg gaccgaagcg atgatcctcc
     2821 ctatcgcggg ggatgtcggc gtagcgcgtg acctgtttgg cggcgctgcg atcgaaaagc
     2881 tgatcgcaga actgcgacag catttcgatt tgatcgttct tgacacagcc cctgtgctac
     2941 ccatcgccga cacccggatg atcgcgcatc aggcggatgc ggtgatcgtc ctggcgcgct
     3001 ggtcgaagac cccgcgcaag gccatcgaaa gctcgttgcg tcttctggcc tcaaccggag
     3061 ctttcatcgg aggcgtcgtc ttgaccaagg tcgacatgaa ggcgcaggcc cgaacgggct
     3121 acggggacag cggctactac tacaaggact acaaggccta ttatggctga gactctgacg
     3181 gcgtggaggg ggccgccggg cgatttccgt tggcggccgt ggctgcacgc cggacggggc
     3241 ctgtcggccc gcgagctgga gctcatctgg gcggtcttgg cccagatatt catcgctcac
     3301 ctcctctttg gcgcaaaccg gaacgacctt tcactcgctt tcgcccttgt gcaagcggcc
     3361 ctcgtcctgc gcgcgctgac cgcgccatgg gcgcgacagg cgctgagacc ggggcgcctg
     3421 ctgctgcccg gcattttgta cgctattgtc ctggtgtttc tcgtgatcag tctgatccct
     3481 ggcgaaaacc cgagactctt cgcggcattt ccatggctcg tcagtccgac cggctcgatc
     3541 aacaccgagg cttcgctcat cgcgctctgc cgaatgggcg gtctggcctg cgttttcatt
     3601 cagggcattt tgatcggcgc gtcacggtca cgtgcggcct tcttcatgaa cgccttcgtc
     3661 gccgctgcaa cgctctatgc cggggtcgcg atcctgggct tctggattgc gccggatacg
     3721 ctttatggag tggatcgccc ctaccactcg gaccggctcg ccggcacatt ccttagcgcc
     3781 aactcggcag gggccttctt cgggatggcc ctcgtcgcct cgttcggcct gcttttccgt
     3841 cgggggcggt cctctcgagt ttcgggccgc aggatcgcag tgagcgtttc tgtcgcgctt
     3901 cttgccatcg cactgcttct gaccgcgtcg cgcggagcgt tggcggcgac cctcgcagca
     3961 agtgcctttc tgatgttggg tcgtgcggcc atggcccgcc gaacaggttg ggtcaggtcc
     4021 tccctgatgg cggccacagg cgccgccatc atcgtcgttg cggccgtcct gctgtcaccg
     4081 atctctggtc gcctgagtgc ggcggccacg gacatcgacg tgcgtgcggc gatcatcaac
     4141 gcccactggc ccgccattcg gtccgagctc tggcagggct atggtgcagg ggcttttgaa
     4201 cgcgtgaatg cgatggttca gacaccgtcg acggtcgacc gcctgtggtc ggtgcgagcc
     4261 ctgcacaacg tctaccttca gtggctggag gaagccgggc ttgtcggcac actcgccatg
     4321 tggggcgtaa tcgccgtcgt cctgggcggg atcacatcgc atttgctggg ccgcaggcca
     4381 tcgcgctggc cgcccgtggt gatcgcgatg agtgtaatga tcctgctgca cggttttgtc
     4441 gactacgggc tggaagttcc atcgctctct gcccagtggg cgctattcct ggggctcggc
     4501 tacgcctcca cgatgccgcg tcttcgaggt cagaacaaac gaggcgggat caaacccggc
     4561 ttcagtctct cgcgtcggcc gcgtcttcgt cttggcggcg tggcgatttg cgccagcgcg
     4621 acactccttc tcgcaggcgg gattctggcg acgggcattg tgcggcagaa tcgggcgacc
     4681 gaagtgcaaa gcctcgatgc cttgcgggag gcgacacgcc gctcacctgc agatggtcaa
     4741 gcttggctca tcgcggctgt ggccgagcga aacgccgcga agcgctgtgc ccgtctatgc
     4801 gtcaccgatc tgagcaactc ctacaacgcc gccccactgt cccgcaccat cttcgcctgg
     4861 cgaacccgct ttgtgctgga acattgggat caggttccag tgacgttgcg agtgtccgcg
     4921 ctcaggcaca tccatgccca atggcggaca cgatccggtc gtcgagacat cgagggcatg
     4981 agccgtgtcg tcttcaatcc cgcgggccgg cttgcaatca agatcgctgc gaacggaacg
     5041 gctccgggcg actccagcgc cgcagtagag gcagcagagc agggtccaaa t
//
LOCUS       DASVTM010000430         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_360032,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000430 DASVTM010000000
VERSION     DASVTM010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360032"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF633_11840"
     CDS             <1..660
                     /locus_tag="VF633_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8471794.1"
                     /translation="HCEGEKVWRLYQNRAENPVNFAPEQTPARLERTRGALMQEVRMR
                     PGDVLYLPRGWYHDALAQADASLHVTFSVTPLYGRAIFSLLENAALQDPAFRAWLPPA
                     HHDGGRALQSHLADLGRRLSALAADPQFADDLAMTQERLMQPVGRYGLPTRKMLTRYR
                     RTNLIPPSFMGPVAIAMNWAFSQADFAVEDLCALFDFIDDDTILKAVQTATSAGALVR
                     T"
     gene            683..1540
                     /gene="gluQRS"
                     /locus_tag="VF633_11845"
     CDS             683..1540
                     /gene="gluQRS"
                     /locus_tag="VF633_11845"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX8471795.1"
                     /translation="MTFVTRFAPSPTGLLHRGHAFSILTVSAAMQEAAKTEGARFLLR
                     IEDTDFTRCRPEYEAAIIEDLVWLGIDWEQPLRRQSEHRADYDAALERLRDMGVLYRC
                     FRTRKELMALAGVAPHSDDPNDPHAFSYGPMATDEEAERLARGEAFAWRLSLDAARDR
                     LGDLSALTWVEERVRPGVHHATPERLGDMVVGRKDIGAGYIIAAVIDDALQGVTHVIR
                     GEDLIEVTSVQRVLQALLDLPTPVYRHHPLLLGPDGKRYAKRDKSVALAELRASGITA
                     EALKAEIGF"
     gene            complement(1537..2490)
                     /locus_tag="VF633_11850"
     CDS             complement(1537..2490)
                     /locus_tag="VF633_11850"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEX8471796.1"
                     /translation="MRRRDLLFRLGLIGATVAGGWWFRDNVLWRKPDIFLPTEPEWLP
                     FAERRASVPTVEVLLGDRPIRALIDSGAQYSVIDRALLAEIGAPETFDMPIVAYGVGG
                     QAQVGKGVTLDVTVGATKVSHLRTAILALGPLASKAGLGTSLILGQDVLGELMLDLDV
                     ERRRLRFLPANAGSGAAGWPDNVAAVDVRRERGALKTTVTVEGATVEAVIDTGSSAII
                     ALSRSTATAAGLLDERERRDGTSIVLGGSMASTIVQARTMTFADQLYERAEAAVYADV
                     ALPGFPDALVGMAAFAGRRAVLDLGGGRMWASRQLDLTVGR"
     gene            2709..>3625
                     /gene="thrC"
                     /locus_tag="VF633_11855"
     CDS             2709..>3625
                     /gene="thrC"
                     /locus_tag="VF633_11855"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX8471797.1"
                     /translation="MTDAAARYISTRGNTTPTTFADALLRGMAPDGGLYMPSAWPTLP
                     AGATAPGQSYVQIAKAAMAPYIGDALPEGALDRALARLVAGFDRPDVTPLVQLSDDLW
                     MLELWHGPTAAFKDLAMQLAAALSEEALEASGERLTLVTATSGDTGAAAVNAFANKDR
                     IDLVVLHPLDRVSAVQRRQMTTVEADNVLNLAVRGDFDDCQRLVKGLLADESLRDRGR
                     FSSVNSINWARLAGQIPYYVSASMQLAAAGETRAPTFVVPTGNFGDAFAGIAARKMGL
                     PVANFVAAVNQNDALARAINDGVYARRSA"
BASE COUNT          649 a         1214 c         1121 g          641 t
ORIGIN      
        1 cactgcgaag gtgagaaagt ctggcgcctc tatcagaaca gggctgaaaa cccggtgaac
       61 ttcgctccgg aacaaacccc ggcaaggctg gagaggaccc ggggcgcgct gatgcaagaa
      121 gttcgaatgc gtccgggcga cgtcctctac ctgccgcggg gctggtatca cgacgcgctc
      181 gcccaggcag acgcttcgct gcacgtgacc ttctctgtca ctcctctcta tggccgggct
      241 attttctcgc ttctggaaaa tgcagcgctg caggacccgg cgttccgcgc gtggttgcca
      301 cctgcgcatc acgatggggg cagagccttg caatcacatc tggccgatct gggtcggagg
      361 ctttccgcat tggcggccga tcctcagttt gcagatgatc tggccatgac ccaggaaagg
      421 ctgatgcaac cggtcggtcg ctacggcctt ccaactcgaa agatgctaac cagatatcgt
      481 cgcaccaacc tgatcccgcc tagcttcatg gggccagtcg caatcgccat gaactgggcg
      541 ttttcacaag cggatttcgc ggtcgaagac ttgtgcgcat tgttcgattt catcgacgac
      601 gacaccatcc tgaaagcggt tcagacagct accagcgcag gcgcactggt gaggacttga
      661 tgttgcgaat ggcacgctac ggatgacgtt tgtcacgcgg ttcgcgccct ccccgaccgg
      721 cctcctgcat cggggtcatg ccttttccat cctgaccgtc tcggccgcga tgcaggaggc
      781 ggccaaaaca gaaggcgctc gctttctcct gcggatcgag gacaccgatt tcactcgttg
      841 ccgtccggag tacgaggcgg cgatcatcga ggatctggtc tggctcggca tcgactggga
      901 gcagcccctt cggcgtcaat ccgaacatcg cgcggattat gacgcggctc tcgaacggct
      961 gcgcgacatg ggagtactct atcgatgctt tcgaacgcgc aaggagctga tggcgctcgc
     1021 tggtgtcgcg ccgcattccg acgacccgaa cgatcctcat gccttcagct atggtccgat
     1081 ggcgactgac gaagaggccg aacgtctggc gcgcggtgag gctttcgcct ggcgactgtc
     1141 gctggacgca gcgcgcgatc ggctgggtga cctgtcggcc ctgacctggg tcgaggaacg
     1201 cgtgcgcccc ggcgtccacc acgcgacgcc tgaacggttg ggcgacatgg tcgtcgggcg
     1261 taaggacatc ggcgccggat acatcatcgc cgccgttatc gacgacgcct tgcagggtgt
     1321 gacgcacgtg atcaggggag aggacctgat cgaggtcacg tctgtccagc gggtgctaca
     1381 ggccctgcta gatctcccga ctcctgttta tcgtcatcac ccattgctgc tcggccctga
     1441 cggcaagcgt tacgctaaac gcgacaagtc ggtggccctc gcagaactac gggcgtccgg
     1501 catcacggcc gaggccctga aagccgagat cggtttctag cgcccgacgg tcaggtccag
     1561 ctggcgcgac gcccacatcc gtccgccgcc cagatcgagg accgctcgtc gtccggcgaa
     1621 ggccgccatc ccgaccaaag cgtcggggaa gcccggaagg gcgacgtcgg catagaccgc
     1681 cgcctcggcc cgttcataga gctggtcagc gaaggtcatg gttctcgcct gtacgatggt
     1741 cgaggccatg gagccgccga gcacgatgct ggttccgtcg cgacgctcgc gttcatccag
     1801 cagtccggcg gctgtcgcag tcgatcggct gagtgcgatt atcgctgacg agccggtatc
     1861 gatcacggct tcgaccgtcg caccctcgac ggtgaccgtg gtcttcaacg cgccgcgttc
     1921 acgacgcacg tccacagcgg cgacgttgtc cggccatcca gcggcgccac tgccagcatt
     1981 tgcaggcaga aaccgcagcc gacgccgctc gacgtccaga tccagcatca gttcgcccag
     2041 cacgtcctga cccaggatca acgacgtccc gagaccggct tttgacgcca agggcccgag
     2101 cgccaggatt gccgtgcgca gatgggaaac cttggtcgcg ccgacggtga catcaagcgt
     2161 aacccccttg ccgacctggg cctggccacc gacgccgtat gcgacgatcg gcatgtcgaa
     2221 tgtctcgggc gcgccgatct cggccagcaa ggcgcgatcg atcaccgaat actgggcccc
     2281 actgtctatc aaagctcgga tcggtcgatc ccccagcagg acctcgaccg tcggcaccga
     2341 ggcacgccgc tccgcgaagg gcagccattc cggctcggtc ggcaggaaga tgtcaggctt
     2401 gcgccacagg acgttgtcgc gaaaccacca gccgccggcc acggtcgcac cgatcaaccc
     2461 cagccgaaac agaaggtccc gacgtctcac gccttgcgcc tgatagggcc gttgcatttc
     2521 atctcaccag acatggccgg tcacatcgaa agacgccaga ccccacgcgc acattcgccc
     2581 ggccgtcatg tcgcaggctc agcccttgcc gccatcagcg cagatcgccg gaacttgcag
     2641 gagaaagggc gcctgttgcg gcggaatgcg ggtgacgccg ccattttggc tggctacgaa
     2701 acatagccat gaccgacgcc gccgctcgct acatctcgac ccgtgggaat acgaccccga
     2761 cgaccttcgc tgacgccctg ctgcgtggga tggcgccgga tggcggcctc tacatgccct
     2821 cggcctggcc caccctgccc gccggagcga cggctcccgg ccagtcctac gtccagatcg
     2881 cgaaagcagc gatggcgccc tatatcggcg acgccttgcc tgaaggggcg ctcgaccggg
     2941 cgctggctcg tctggtcgcc gggttcgacc gccctgacgt gaccccactg gttcagctca
     3001 gtgacgatct ttggatgctc gagctatggc atgggcccac ggcggccttc aaggatctgg
     3061 ccatgcagct ggccgccgca ctgtccgaag aggctctgga agcttcgggc gaacggttga
     3121 cgctcgtcac ggcgaccagt ggtgacaccg gcgcagcggc cgtcaacgcc tttgcaaaca
     3181 aggaccgcat cgacctggtc gtcctgcacc cgctcgaccg ggtctccgcc gttcagcgcc
     3241 gacagatgac gacggtcgag gctgacaacg tcctgaacct ggccgttcga ggcgatttcg
     3301 atgactgcca acgactggtg aaaggcctgc tggccgacga aagcctgcgt gatcgtggcc
     3361 ggttttcctc ggtcaactcc atcaactggg cgcggctcgc cgggcagatc ccctattacg
     3421 tctctgcctc catgcaattg gctgcggcgg gcgagacgcg cgctccgact ttcgtagtcc
     3481 cgaccggcaa tttcggcgac gccttcgccg gcatcgcggc gcgcaagatg gggctcccgg
     3541 tcgcgaactt cgtcgcggcg gtgaaccaga acgacgctct ggcccgcgcc atcaacgacg
     3601 gcgtctatgc tcgacggtcg gccat
//
LOCUS       DASVTM010000431         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_97166, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000431 DASVTM010000000
VERSION     DASVTM010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97166"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VF633_11860"
     CDS             <1..353
                     /locus_tag="VF633_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471798.1"
                     /translation="TYSDVETESGDPLPGNSKNSYNAAVYYEDDVLSARLAYNYRSDF
                     FVTFDRSTQLNQDEIGSLDGSISWNVTRQVALTLEGQNLLDENIEQFAGNTIRPRAVY
                     DNGRSIYAGVRYRY"
     gene            474..>2211
                     /locus_tag="VF633_11865"
     CDS             474..>2211
                     /locus_tag="VF633_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX8471799.1"
                     /translation="MLNSSTVVLVALVLSLGGCAVLKAEPVSGAAPVVTEAPVAAIIP
                     QPASVEAAQGRYILKDGARLVVPVNDTKARAAADRFAGLMGRTRNLSVIVTDEGVGDI
                     VLSRAPLNKAESYDLAVAPDRVSISAGDDAGLQYGLITLWQLATQTPTGPAPIAAVRI
                     TDAPRFAWRGVMLDSARHYQSPEFIKGFIDWMALHKLNVFHWHLTDDQAWRLEIRSHP
                     KLTEIGAWRVPEGHAAQTDIDPATGQPRLYGGFYTQAQVREIVAYAAARGITVVPEID
                     VPGHASAAVAAYPELGVRDAPDSAVAAVPSDWGVYHTLFNTKPETIVFLTSVLDEVMD
                     LFPGQYIHLGGDEAAKDQWKASPSTQAHMRALGLTDEMQLQGWFMKQMETHLNAHGRR
                     LIGWDEILEGGLEGSATVMSWRGVEGAIEAARLGHDTVLTPGPTFYFDYRQSPNDGAP
                     GRVVMSTMQGLYDFDPWAGMTPEQAKHVLGVQGNIFTEHMLTEDRVEYMAFPRTAALA
                     ELAWSAPERTDFSDFTRRLQPQLARYRALGIGYAEAALDTVAEAPPPDGERRYDHQMA
                     LCSSAIAIALIDD"
BASE COUNT          391 a          723 c          713 g          384 t
ORIGIN      
        1 acacctattc ggacgtcgag acggaaagcg gcgatccgct gccgggcaac tccaagaact
       61 cgtacaacgc cgccgtctac tatgaggacg acgttctttc ggcgcggctg gcctacaact
      121 atcgctcgga cttcttcgtc accttcgacc gctcgaccca gctgaatcag gatgagatcg
      181 gatcgctgga cgggtcgatc tcgtggaacg tgaccagaca ggtcgccctg acgctcgaag
      241 gccagaacct gctggacgag aatatcgaac agtttgcagg caatacgatc cggccccgcg
      301 cggtctacga caatggccga tcgatctacg cgggcgttcg ctaccgctac tgaccaaccc
      361 tgacgactgg gcaggaggcg gttcgctgtc tcctgcctgt ttttcccggg cgcgccgacc
      421 taggcttcgc catccgcgag gtcatcggga gaccgccgga tcggaagcgt agaatgctga
      481 actcctctac tgtcgtgctg gttgctctcg tgctgtccct cggaggatgc gcggtcctga
      541 aggctgagcc tgtttcaggc gccgccccgg tcgtgaccga agctccagtc gccgcgatta
      601 tcccgcaacc ggccagcgtc gaggctgctc aagggcgcta cattctcaag gatggcgccc
      661 ggctggtcgt tcccgtcaac gacaccaagg ccagagcggc cgccgaccgg tttgcgggtc
      721 tgatgggccg cacccggaac ctgtcggtga tcgtcacgga cgagggcgta ggcgatatcg
      781 tcctttcccg cgctccattg aacaaagccg agagctacga tctggccgtc gcgccggatc
      841 gcgtctcgat ctcggccggc gacgacgcag gcctgcaata cggtctgatc accctgtggc
      901 agctggcgac ccaaacgcca acgggtccgg cgccgatcgc cgccgtgcgg atcactgatg
      961 cgccgcgttt cgcctggcgc ggtgtcatgc tggattcagc gcgccactat cagtcgcccg
     1021 agttcatcaa ggggttcatc gactggatgg ccctgcacaa gcttaatgtc ttccactggc
     1081 acctgaccga cgatcaggcc tggcggctgg agatccgcag ccatcccaag ctgaccgaga
     1141 tcggcgcatg gcgcgtgccc gaaggccatg ccgcccagac agacatcgac ccggcgaccg
     1201 gacagccgcg tctctacggc ggcttctaca cccaggctca ggtgcgcgag atcgtcgcct
     1261 atgccgcggc gcgcggcatc accgtcgtgc ccgaaatcga cgtgccggga cacgccagcg
     1321 cggctgtcgc ggcctatccc gaactcggcg tgcgagacgc gccggacagc gccgttgcgg
     1381 ccgtgccgtc cgactggggc gtctatcaca ccctgttcaa tacgaagccc gagaccatag
     1441 tcttcctgac ttcggtgctg gacgaggtga tggatctgtt tccggggcag tacatccatc
     1501 ttggcggcga cgaggcggcc aaggaccaat ggaaggcctc gccgagcact caggcgcata
     1561 tgcgcgcgct gggcctgacc gatgagatgc agctgcaggg ctggttcatg aagcagatgg
     1621 agacccatct gaacgctcac ggtcgacgcc tgatcggatg ggatgaaatc ctcgaaggcg
     1681 gtctggaggg gtcggcgacc gtgatgtcat ggcgcggcgt cgagggcgcg atcgaggcgg
     1741 cgcggcttgg ccacgatacg gtcctgacgc ccggacccac cttctatttc gactatcgcc
     1801 agagccccaa cgacggggcg ccggggcggg tggtcatgtc gaccatgcag ggcctctacg
     1861 acttcgatcc gtgggcgggg atgacccccg agcaggccaa acacgtgctg ggcgttcagg
     1921 gcaacatctt caccgagcat atgctgaccg aggaccgggt cgaatacatg gctttcccga
     1981 ggaccgccgc gctggcggaa ctggcatggt cggcgcccga acggacggat ttctccgatt
     2041 tcacccgtcg gctgcagccg caactggcgc gctatcgggc gctggggatt ggctatgccg
     2101 aggccgcgct ggatacggtg gctgaggccc cgccgcccga cggggagcgt cgttacgatc
     2161 accagatggc cctgtgcagc agcgccatcg ccatcgccct gatcgacgac g
//
LOCUS       DASVTM010000432         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1052353,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000432 DASVTM010000000
VERSION     DASVTM010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052353"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..874)
                     /locus_tag="VF633_11870"
     CDS             complement(68..874)
                     /locus_tag="VF633_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8471800.1"
                     /translation="MLLITSGDVTPAVQVVYEPMLCVILEGAKQTTLGAESYVYHAGD
                     YLVVSADLPVSGQVMQAPYTAVGIPLSPAAIAELMIETGSSTSEEAPKRALAVSRMDD
                     DLINSVGRLIRLLERPQDVAVMAPMIRREILWRLLAGEHGAMVRQIGAEDGRLGRVRR
                     VIRSLRQDYAKPIRIEDLAELAGMSETSLHRHFKAVTSMSPLQFQKQVRLQEARNRLL
                     GQGGDVAGVGFAVGYDSPSQFSREYSRQFGRPPGRDLERLRADPTLAMAV"
     gene            1109..1993
                     /locus_tag="VF633_11875"
     CDS             1109..1993
                     /locus_tag="VF633_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX8471801.1"
                     /translation="MTTTTPHTWFITGAARGLGAAIARAALDAGDNVVVAGRNRDALI
                     KVVGEDSDRVLSVTLDVADLAQAQPAVDAALARFGRIDVLVNNAGYGHLGMFEESTAA
                     DVQAQYDTNVFGLMSVTRAVLPAMRAQRSGRIFNVSSIGGIVGGTSGTLYCASKFAVE
                     GFSESLAQEVGAFGIQVTIVEPGFFRTDFLEPTSVKHGTNPIPDYAEAAAALKTFYDA
                     RSRNQAGDPARLGLALVALVNAPQQPTRWAAGSDGLSMVQSKITSLQAELDAWKDLTL
                     STDGDFAFAPEPGSSAWG"
     gene            complement(2001..2552)
                     /locus_tag="VF633_11880"
     CDS             complement(2001..2552)
                     /locus_tag="VF633_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX8471802.1"
                     /translation="MSQTPPTLSVSALRWFGKTPDKLTDRQRRVLERALARKTVSKNM
                     NVAFDGEMTFGERLADHVASFGGSWKFIILFGVILAVWVGLNLLLARAAFDPYPFIFL
                     NLMLSMLAALQAPVIMMSQNRQSAKDRLDAGHDYEVNLKSEIEIMALHDKLDQMRTQE
                     LEVLVRQQGEQIALLTRLVEARG"
     gene            complement(2618..>3108)
                     /locus_tag="VF633_11885"
     CDS             complement(2618..>3108)
                     /locus_tag="VF633_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF817 family protein"
                     /protein_id="HEX8471803.1"
                     /translation="IGEVPLFSGFMYAAVGSYIARIMRIFDIRYRHYPPLWTTWLLAA
                     AIYINFFSHHWLPDIRNILFVATIVLFGRGWFFFTPDRQRRSMPFLLGFFLVASFIWL
                     AENLGTLARAWVYPGQEDGWSPVGFAKLGSWFLLMMISVVLVSLVHRPQAEPVRTDPS
                     LE"
BASE COUNT          597 a         1125 c          879 g          507 t
ORIGIN      
        1 cgtggacgat atcgaagaga tcaaggccgc aagctgatct cgatacggcg ccacccgcgg
       61 cacgggccta aaccgccatc gccagcgtcg gatcggcgcg caaccgttcc agatcgcggc
      121 ccggaggccg gccgaactga cggctgtatt cgcgactgaa ctggctcggg ctgtcatagc
      181 ccacggcgaa cccaacaccc gccacatcgc caccctgccc cagcagccgg ttgcgcgcct
      241 cctgcagccg cacctgcttc tgaaactgca gcgggctcat cgaggtcacg gccttgaagt
      301 ggcgatgcag cgaggtctcg ctcatcccgg ccagttccgc cagatcctcg atccggatcg
      361 gcttggcgta gtcctgacgc agcgatctga taacccgccg gacgcgaccc agccgtccgt
      421 cctccgcgcc gatctggcgc accatcgcgc catgctcgcc agccagcagc cgccacaata
      481 tctcgcgccg gatcatcggc gccatcaccg ccacgtcctg cggtcgttcc agcaatcgga
      541 tcaaccggcc cacgctgttg atcaggtcgt cgtccatccg gctcaccgcg agcgctcgct
      601 tcggcgcctc ttccgaggtc gacgaccccg tctcgatcat cagttcggcg atggcggcgg
      661 ggctcagcgg aatgccgacc gcagtatagg gcgcctgcat cacctgaccg ctgaccggca
      721 gatcggccga gaccaccaga taatcgcctg cgtgatagac gtagctctcg gcacccaacg
      781 tcgtctgctt cgccccttcg aggatcacgc acagcatggg ctcatagacc acctgcactg
      841 cgggtgtgac gtcaccgctg gtgatcaaca gcatccccgg acagtcttct ggcgagaccc
      901 cgagtgcagc gtgacggcgg gcgaggtcag cgagttcgtg caatcgatcc atgagagcgt
      961 tcaattcctg acggctcact cagtgcaagt cgctgacatc caatctgatc gccttggcag
     1021 gatcgtgcaa gagactggcg ccatccgtct acgccccgac gccgcctctg gcccataaga
     1081 cctcatccca accagaggac aaaccatcat gaccaccacc accccccaca cctggttcat
     1141 caccggcgca gcccgcggcc tcggcgccgc aatcgcccga gcagcgctcg acgcaggaga
     1201 caatgtcgtc gtcgccggtc gcaatcgcga cgccctcatc aaggttgtcg gcgaggactc
     1261 cgaccgcgtc ctgtccgtca ccctcgacgt cgccgatctg gctcaggccc agcccgccgt
     1321 cgacgccgcc ctcgcccgct ttggccgcat cgatgtgctg gtcaacaatg ccggctacgg
     1381 ccatctcggc atgttcgagg aatccaccgc cgccgacgtt caggcccaat acgacaccaa
     1441 tgtcttcggc ctcatgtccg tgacccgagc cgtcctgccc gccatgcgcg cccaacgttc
     1501 gggccgcatc ttcaacgtct cctccatcgg tggcatcgtc gggggcacca gcgggaccct
     1561 gtattgcgcc tcgaaattcg cggtcgaagg cttctcggaa tcgctggcgc aggaggtcgg
     1621 tgccttcggc atccaggtca ccatcgtcga acccggcttc ttccgcacgg acttcctcga
     1681 gcccacctcg gtcaagcatg gcacgaaccc gatccccgac tacgccgagg ccgccgccgc
     1741 attgaaaacc ttctacgacg cccgcagccg caatcaggcc ggcgatccgg ccaggctcgg
     1801 cctcgccctc gtcgctctgg tcaacgcacc acagcaaccg acccgctggg ccgctggttc
     1861 agacggcttg agcatggtgc agtcgaagat caccagcctt caggccgaat tagacgcctg
     1921 gaaggacctg acactcagca ccgacggcga cttcgcgttc gccccggaac ccggaagcag
     1981 cgcctggggc tgatcgccgg ttagccgcgc gcctcgacca atcgcgtcag cagggcgatc
     2041 tgttcgcctt gctgacgcac cagcacctca agctcctgcg tgcgcatctg atccagcttg
     2101 tcgtgcaggg ccatgatctc gatctccgac ttcaggttca cttcatagtc gtgccctgcg
     2161 tcgaggcgat ccttcgccga ctgccggttc tggctcatca tgatcaccgg cgcctgcagc
     2221 gcagccagca tcgacagcat caggttgagg aagatgaacg gatagggatc gaacgccgcc
     2281 cgcgccagca gcaggttcag cccgacccag acggccagga tcacgccgaa caggatgatg
     2341 aatttccacg atccgccgaa cgaagccaca tgatccgcca gccgctcgcc gaaggtcatc
     2401 tcgccgtcga aggcgacgtt catgtttttc gagaccgtct tgcgcgccag cgcccgctcc
     2461 agaacccgcc gctgacggtc cgtcagcttg tcaggagtct tgccgaacca gcgcagcgcc
     2521 gagacggaca gggtcggagg ggtctgggac atgaggggct ttctggatca cgcgggacgc
     2581 aaccaacgtt ccacccgaca gcgccattac gccagttcta ttccagagac ggatcagtcc
     2641 gcacaggctc cgcttgcggc cggtgtacca acgacaccag caccacgctg atcatcatca
     2701 gcaggaacca gctccccagc ttggcgaacc cgaccgggct ccagccatcc tcctgccccg
     2761 gatagaccca cgcccgcgcc agcgtcccga ggttttcagc cagccagatg aagctcgcca
     2821 cgaggaagaa ccccagcagg aacggcatcg atcgcctttg acgatccggc gtgaagaaga
     2881 accatccgcg cccgaacagc acgatggtcg ccacgaacag aatgttgcgg atgtccggca
     2941 gccagtgatg tgagaagaag ttgatataga tcgccgccgc cagcagccac gtcgtccaca
     3001 gcggcggata gtgccgatag cggatgtcga agatccgcat gatccgcgcg atgtacgacc
     3061 cgaccgccgc atacatgaag cccgagaaca gcggcacttc gccgatcc
//
LOCUS       DASVTM010000433         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_627903,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000433 DASVTM010000000
VERSION     DASVTM010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627903"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VF633_11890"
     CDS             complement(<1..381)
                     /locus_tag="VF633_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX8471804.1"
                     /translation="MNSLALRSEDFLAGAVEYGADEWLPDDAPLDMPRQDFDVGSAMV
                     STPVATSPVNVAGRRLILLSATVIVTALAAITPFILYARHGFDPLEMVAFGLFLILIV
                     SIACWFSNSAIGLWVLMTNREQDDL"
     gene            complement(511..2337)
                     /locus_tag="VF633_11895"
     CDS             complement(511..2337)
                     /locus_tag="VF633_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX8471805.1"
                     /translation="MTAPSQSPDTAPDKSGSLSTSRRRHDLDWIRIAAFALLIVYHVG
                     LAFGPFDWHVHSAHTLEWVREGVLITNPWRLTLLFLVSGAALRFMTLRKTPAEVARLR
                     LARLGPPLVFGVLVLVTIQSWIEAMDKSHEAISYPAWLWHEFSPSGLADGVPLNHLWF
                     VLYIAVYSFIVVALLNKPGWIAAAEAKIGPALRGWRLVAFPALYLMFVRTWLFPHFGL
                     TNNIVWDWYNHAQSLPAFLFGFLAVRQESIWKDFQRFRWIGLGVAVVALPVMMLQVAH
                     PGGGAYGGIPRNAVVALDQWAVMVAVLGFASLYLHKASSPALAYLNDAVFTLYLAHQT
                     ILVCAVWLIRPAALPVWIEAPILIAVTIGGSLLIYEIVRRVPLLRPIWGLKPLPGRDV
                     FSGLALSRYRRRRILLGVGVFAPVLAFVVVGLAILSYPGFNNARQYLSELGGASSPMP
                     MIFNWGVFVAGVMAGIAGVGFGLAVIAITRAHIAGWLTAIVFILAGTGLALSTLFPYP
                     DPRHMYINMGLGIQIAPLLLLWGLAGSREMPRLKAFLIGVFVVMTGLTVVTYHLVLPG
                     TVNPGNVGWWERVYAIVLVGWVGVAAWVLGRRLRRQAEADES"
     gene            2536..3186
                     /locus_tag="VF633_11900"
     CDS             2536..3186
                     /locus_tag="VF633_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8471806.1"
                     /translation="MQWIVLIVDDHPLVGEAFEISVAAAYPHLEVGRVTTAAEAEMYA
                     RANASRIKLVLLDLMLPDATGFSALILLQQLLPGIPIAIVSSRADAHTVALARTFGVS
                     AYLSKSTPVSSLVNAVGTLLRGEQVPFPEAGPATPVAVDFQQRVSTLSAAQLRVLVAL
                     ADGRLNKQIAGDMNLTEGTVKQHLSAIFKKLGVGNRSQAILAARPYLKGLDAGEED"
     gene            3288..>3425
                     /locus_tag="VF633_11905"
     CDS             3288..>3425
                     /locus_tag="VF633_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX8471807.1"
                     /translation="MRIGLYPGTFDPVTNGHMDIIGRALKLVDRMVVGVAQNDEKGPL
                     FS"
BASE COUNT          728 a         1077 c         1061 g          559 t
ORIGIN      
        1 gaggtcgtcc tgctcacggt tggtcatcaa gacccacagg ccgatggcgg agttggagaa
       61 ccagcaggcg atcgagacga tcagaatcag aaacaggcca aacgccacca tctcgagcgg
      121 atcgaagccg tggcgggcat agaggatgaa gggcgtgatc gcagcgagag ccgtgacgat
      181 cacggtggcg ctcagcagga tcagacggcg cccggcgaca ttgaccggcg aggtggctac
      241 cggcgtcgaa accattgcgg atccaacgtc gaaatcctgg cgaggcatgt cgagaggggc
      301 gtcgtctggc agccattcat cggcgccgta ttcgacggct ccggccagaa aatcctcgct
      361 acgcagggcc aggctgttca tggcgtcttc gatttcctga aaaagccgcg ctcaaggcgg
      421 gtgcgcttgg actactgcaa tgcagcatga acgcattttc gtgaaaatca atcacagagt
      481 tgtgatagcg ccgtgttttt tatgcgggaa ctaactctcg tcagcctctg cctgacgacg
      541 caggcggcgg ccgagcaccc aggctgcaac tccgacccaa ccgaccagca cgatggcgta
      601 gacccgctcc caccaaccga cattcccggg gttcacggtc cctggcagga ccagatgata
      661 ggtcaccacg gtcaggcccg tcattaccac gaaaaccccg atcagaaagg ctttcagccg
      721 cggcatctcg cgacttccgg ctagtcccca cagcagcagc aggggcgcaa tctgaatgcc
      781 cagacccatg ttgatgtaca tgtggcgggg gtccggatac gggaacaggg tcgacaaggc
      841 gaggcccgtt cccgcgagga tgaagacgat cgcggtcagc catccagcga tgtgagcgcg
      901 tgtgatcgcg atcacggcca gcccgaagcc gacacccgca atccccgcca tgacccccgc
      961 gacgaagacc ccccagttga aaatcatcgg catcggcgat gacgcgccgc ccaactcgct
     1021 gaggtactga cgggcgttgt tgaagccggg ataggacagg atggccagtc cgaccacgac
     1081 gaaggcgaga accggtgcaa acacgccaac gccgagcagg atacggcggc ggcgatagcg
     1141 gctgagcgcc aaacccgaga acacatcacg gccaggcagg ggcttcagcc cccagatcgg
     1201 tcgcagcaag gggacacgcc tcacgatctc gtagatcagc aaactgcccc cgatcgtcac
     1261 cgcgatcagg atcggcgcct cgatccatac cggcagagcg gccgggcgga tcagccagac
     1321 ggcgcagacg agaatggtct ggtgcgccag atacagggtg aagaccgcgt cattgaggta
     1381 ggccagcgca ggactggacg ccttatgcag atacaggctg gcgaacccga ggactgcgac
     1441 catcaccgcc cactggtcca gcgccaccac tgcgttgcgc ggaatgccgc cataggcgcc
     1501 gccgcccgga tgagcgacct gcagcatcat cacaggcagg gcaaccaccg ccacaccgag
     1561 gccaatccag cgaaaacgct gaaagtcctt ccagatgctc tcttgccgca cggcaaggaa
     1621 gccgaacagg aaggcgggga gtgattgggc gtgattgtac cagtcccaga cgatattgtt
     1681 ggtcaggccg aaatgcggga acagccacgt cctcacgaac atcaggtaca aggccgggaa
     1741 agccaccagc cgccagccgc gcaaggcagg gccgatcttc gcttcggccg ccgcgatcca
     1801 cccgggtttg ttcagcagcg cgacgacgat gaagctgtac accgcgatat aaaggacgaa
     1861 ccacaggtgg ttcagcggca ccccgtcagc cagacctgag gggctgaact catgccacag
     1921 ccacgccgga tagctgatcg cctcgtgcga cttgtccatc gcttcgatcc acgactggat
     1981 tgtgaccaat accagcacgc caaacaccag tggcggcccg agccgggcca gacgcaatct
     2041 ggcgacctcg gcgggcgtct tcctgagcgt catgaaccgc aaggccgccc ctgacaccag
     2101 gaacagcagc gtcagtcgcc aggggttggt tatcagcacg ccttcgcgca cccactcgag
     2161 cgtatgagcg ctgtggacgt gccaatcgaa agggccgaag gcgagaccca catgatagac
     2221 gatcagcagg gcgaaggccg ctatgcgaat ccagtccagg tcatgacgac gacgtgatgt
     2281 cgacaatgag cccgacttgt ctggcgcggt atccggactc tgggagggag cagtcacggc
     2341 ctccctatga gcgaggccgc ccgacctgtc cacggcgcta tggcgcgagg ccgggatctg
     2401 ttcagccggg cctgctgtga ggcgctaatc agaacggcgg cgggaacctc ggtatttgcc
     2461 gaatgttcac ctcacggttc tcgttgcctc attccacggt tgtgggaaga tgtgtcaggg
     2521 aggcgattca atcgcatgca gtggattgtc ctgatcgtcg atgaccaccc gctggtcgga
     2581 gaggcgttcg agatttcggt cgccgccgcg tatccgcacc ttgaggtcgg tcgcgtcaca
     2641 acggcggccg aggccgagat gtatgctcgc gccaatgcgt ctcgcatcaa actggtgttg
     2701 ctggacctca tgcttcccga tgcaacaggg ttcagcgccc tgatcctcct gcaacagctt
     2761 cttcccggca ttccgatcgc aatcgtgtct tcgcgagccg acgcccatac ggtggccctg
     2821 gcccgcacgt tcggcgtgtc agcctatctg tcgaagtcga caccggtcag ctcactggtc
     2881 aacgccgtcg gcaccctctt gcgtggcgaa caggtcccgt tcccggaagc cggtccggcg
     2941 acgccggttg cggtagattt ccagcagcgg gtttccacgc tgtcggcggc tcaactgcgc
     3001 gtgctggtcg cgctcgccga cggtcggctc aacaagcaaa tcgccgggga catgaacctg
     3061 accgaaggaa ccgtgaaaca gcatctctcg gccatcttca agaagttggg cgtcggtaat
     3121 cgcagccagg ccattctggc ggcgcggcct tatctcaagg gcctggacgc cggtgaagaa
     3181 gactgacagc gcctcgacgc ttcgcactct tgtctctagg ccgcctgttt cgcacctgct
     3241 aacaccggct tgtgaggcgg aggcgaaacc ggatgcatag cggcaagatt cggatcgggt
     3301 tgtatccagg gaccttcgat ccggtcacca acggccacat ggacatcatc gggcgggctc
     3361 tgaagctggt cgatcgaatg gtcgtcggcg tggcccagaa cgacgaaaag ggcccgctgt
     3421 tctcc
//
LOCUS       DASVTM010000434         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_42504, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000434 DASVTM010000000
VERSION     DASVTM010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42504"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="VF633_11910"
     CDS             <1..1544
                     /locus_tag="VF633_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8471808.1"
                     /translation="SEHDEALRTSALSAASDLRQPDWRAAAFPDEDFSTNSDDIWSTD
                     PLTSELAPAPFPDFAEPETVREPAYDPAKAIAEIFASPRLSEPREEVIQPKFADPTDT
                     PAPVKAEAIAGEVSATPVFGGFGGADVEDALEATAALSPEFVAQRPEPALAPQAPAAF
                     GALPTDKSPAFDPEMAFDAGPDEFSAETDFVDARSMRHGLAAGAAGGRSASTRNAVDA
                     ARAAMTASATETVEEPRGSFGLKRGGKSRLQERLDKQAKGGSTIRKGLLASVTGMALV
                     GGLMTTNRLLGGDSFAIPGLDKVSQILGLAPAPAVAPIDETTPLLAAALTPTDAVPAV
                     PSEAQTLFDEAVEQLDGGAPEGVATLTRAANMGLPAAQLKLVGMYESGTNGVARNLNE
                     ARLWARRAATNGDPKGMHAYGMYLFDGVGGARNRPEALDWLVKAADRGEVDSQYNAAK
                     IYEDGAEGVAPNATEAYKWYLIAARAGDQGAQTAVDRLTPALPAAGRQTARAAADAFQ
                     TEPLA"
     gene            1664..>2182
                     /locus_tag="VF633_11915"
     CDS             1664..>2182
                     /locus_tag="VF633_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8471809.1"
                     /translation="MDIYLPIAEVSVHWPILVLLGTAVGFVSGLFGVGGGFLMAPILI
                     FLGIPPTVAVASQASHVVASSTSGVIRYAGQKAVDFKLGGIMAAGGAIGALVGVEVFR
                     YLRLLGQADLVVALSYLLFLGAIGTLMLNESLTEIIRRRRGETPPHKDRRRPLFLYGL
                     PFRMRFPKSGLYI"
BASE COUNT          359 a          770 c          688 g          365 t
ORIGIN      
        1 catccgaaca tgacgaagcg ctccggacct cggcgctttc cgcggcgtcc gacctgcgcc
       61 agcccgactg gcgcgccgcc gcgttcccgg acgaggattt cagcacgaac tccgacgaca
      121 tctggtcgac cgatcctctg accagcgaac tcgcccccgc acccttcccc gatttcgccg
      181 aacctgaaac cgttcgcgaa ccagcctacg atccagccaa ggccatcgcg gaaatcttcg
      241 cctcgccgcg tctatcggag cctcgcgagg aggtcatcca gcccaaattc gctgatccga
      301 ccgacacgcc tgctcctgtg aaggccgaag ccatcgccgg ggaagtatca gccacgcccg
      361 tattcggagg cttcggcggc gccgacgtcg aggacgcgct ggaagcgaca gccgcccttt
      421 cgccggagtt cgtcgcgcaa aggccggaac ccgccctcgc gccacaggct cctgccgcct
      481 tcggagcctt gcccaccgac aagtctcctg cgttcgatcc ggagatggcc ttcgatgccg
      541 gaccggacga gttctccgcc gagaccgact tcgtcgacgc gcgctccatg cggcacggcc
      601 tcgcagcagg cgccgccggt ggacgatcgg cctcgacgcg caacgcggtc gacgcagccc
      661 gcgccgcgat gacggcctct gctacggaga ccgtcgaaga accccgcgga agtttcggcc
      721 tgaagcgcgg cggcaaatcg cgccttcagg aacgtctgga caagcaggcc aagggcggtt
      781 ccaccatccg caagggtctg ctggcctcag tgaccggcat ggcgcttgtc ggtggtctga
      841 tgaccaccaa tcggctgctc ggcggcgaca gcttcgccat cccgggcctc gacaaggtga
      901 gccagatcct cggcctggct cccgctcccg cagttgcgcc aatcgacgaa acgaccccgt
      961 tgctcgccgc cgcactgacg ccgacagacg ctgtgccggc cgtgccgagc gaggcgcaaa
     1021 ctctgttcga tgaggccgtc gaacagttgg acggaggagc tccggaagga gtcgctaccc
     1081 tgacccgcgc tgcaaacatg ggcttgcccg ccgcccagct gaaattggtc ggaatgtatg
     1141 agagcggtac gaacggtgtc gctcgcaacc tgaacgaggc ccgcctttgg gcgcggcggg
     1201 cggccaccaa cggcgacccg aagggcatgc acgcctatgg catgtatctt ttcgacggtg
     1261 tcggtggcgc caggaaccgg cccgaggcgc tggactggct ggtgaaggct gctgatcgcg
     1321 gggaggtcga cagccagtac aatgccgcga agatttacga agatggcgcc gaaggcgtgg
     1381 cgccgaacgc gaccgaggcc tacaaatggt accttatcgc ggctcgggcc ggagatcagg
     1441 gggcccagac cgcagtcgat cgcctgacgc cggccctgcc cgccgcaggt cgtcaaacgg
     1501 cccgcgctgc cgcagatgcc ttccagaccg aacctcttgc gtgaggatcc gaccttgaaa
     1561 gcgccaaacg cgcgcaccgg gtggtgaagc cgccacccgg acgctagacc ctccccacgc
     1621 cgtccggcgt cctgttattg tccgtcagcc gaaggcgcgt cctttggaca tctatctgcc
     1681 gatcgccgag gtttcggtgc actggccgat cctggttctg ctggggacgg cggtcggatt
     1741 cgtgtctgga ctgttcggtg tgggcggcgg gttccttatg gccccgatcc tgatctttct
     1801 cggcattcca ccgaccgtgg ccgtcgccag ccaggccagc cacgtcgtgg cctcctcgac
     1861 ctctggcgtg atccgctatg ccggccagaa ggccgtcgat ttcaagctcg gcggcatcat
     1921 ggccgccggg ggcgcgatcg gagccctcgt cggcgtcgag gtgttccgct acctgcgtct
     1981 gctgggtcag gccgacctcg tcgtcgccct gtcctacctg ctgttccttg gcgctatcgg
     2041 caccttgatg ctgaacgaga gcctgaccga gatcatccgc cgccgcagag gcgagacccc
     2101 gccccacaag gatcgccgtc gcccgctgtt cctgtacggc ctgccgttcc ggatgcggtt
     2161 tccgaagtcg ggcctctata tc
//
LOCUS       DASVTM010000435         4660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_788672,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000435 DASVTM010000000
VERSION     DASVTM010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788672"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF633_11920"
     CDS             <1..422
                     /locus_tag="VF633_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8471810.1"
                     /translation="RIANGQSPMVFEDGQQRRDFVHVSDVARAFLLALDKPEADGEVF
                     NIGSGEDRTVEEVARLQAASMGRPDIEPSITQQARAGDIRHNIPDLSKARDVLGYEAR
                     QDFGQGLEELAEWVARQEAHDNVAKARHELETRGLVA"
     gene            419..1477
                     /locus_tag="VF633_11925"
     CDS             419..1477
                     /locus_tag="VF633_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8471811.1"
                     /translation="MTKEAGGTLLVTGGAGFIGSNLADRLASEGHQIIVLDALTRPGV
                     ERNLAWLEARHGSRIERLVADVRDEDTVRSAVSRSDAVFHMAGQVAVTTSLVDPIEDF
                     EINLKGTLNVLEAARPKLTPVIFASTNKVYGDLADIDLQLVEGAWTPSDPQVRARGVS
                     EARPLDFHTPYGCSKGAADQYVLDYARSFGVPAAVLRMSCIYGPRQMGTEDQGWVAHF
                     LIRALEGRTISLYGDGRQVRDILNVRDAVDAYIAAWRNIGSVAGRAFNLGGGPSNAVS
                     LRQLIAHIEGLIGRKVAVDYSDWRAGDQKYFVADASAAQSALGLQPPTAWTDGIVQLA
                     EWLAEERGLSITSREVAA"
     gene            1474..2586
                     /locus_tag="VF633_11930"
     CDS             1474..2586
                     /locus_tag="VF633_11930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8471812.1"
                     /translation="MKRGARGLRVLLTADSVGGVWTYALDLAAGLAAKGAEVIIAHMG
                     LPPTEPPALSDGVEIVFTGLPLDWGDVDADALSSAGQALADLTRVRDVDLVHLNSPIL
                     AADVAFHVPVVGGCHSCVATWWDAVEGHRPLPADFIWRTDRLRAGYAACDALIAPTRS
                     FAEATTGRYGQPCQVVWNGRTPPSTGPLSGPRRPVAVTAGRLWDPAKGAATLDTAAGK
                     MRARVEAAGPLANPFDPRPVSFAHLNLLGRLSPGEVAELLSNSAAFVSAARYEPFGLS
                     VLEAAQAGCALVLSDLPVFRELWDGAAVFVDPTDTSALAETLDALLDDPAETRRLGEL
                     AHTRAGRFSLDVMLAGTLAIYADVLDRAARPSEAAA"
     gene            2583..3713
                     /locus_tag="VF633_11935"
     CDS             2583..3713
                     /locus_tag="VF633_11935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8471813.1"
                     /translation="MKIVYFTHSLASCWNHGNAHFLRGVLRELKALGHEVSAWEPEGS
                     WSLQNLLQDAGPSGLEAWRKAYPDLSSRTFTASAEAERGACDGADLVIVHEWNDPELV
                     AAIGRVRAGGARFTLLFHDTHHRAVSEPNAIHAFDLSGYDGVLAFGETLAQVYRRWGW
                     GDQVFVWHEAADIRLFHPPTEECAREGLVWIGNWGEGERTAELESFLLAAAEDAGLPL
                     DIYGVRYPTEALSLLKRYGANYKGWIANASAPAVFARHLATVHVPRRFYVEQLPGIPT
                     IRVFEALACGIPLVSAPWRDTENLFRPGQDFLVAADGEAMAGHLRNLRDDAGLRQSVV
                     AGGLETIRARHTCAHRAQELLAVADRLGVAHADPSPVLETVR"
     gene            3710..>4660
                     /locus_tag="VF633_11940"
     CDS             3710..>4660
                     /locus_tag="VF633_11940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8471814.1"
                     /translation="MKIAFYGSSLLSSYWNGAATYYRGMLSELSKLGWQVTFYEPDAF
                     DRQQHRDIEPPSWAEVVVYEATPEACGAVMARAAEADVVVKASGVGVFDNELLGVMGF
                     ARPDALRIFWDVDAPATLAELRQNPDHPLKAALPTLDHVFTYGGGPPVIAAYLDIGAR
                     DCRPIYNALDPTTHHPTAPDDRFRVDLALLANRLPDRERRIEHYFLEPAALLPDRTFL
                     LGGSGWEDKAMPANVRRLGHVGTAEHNAFNVSPLAVLNVARDSMAEVGFSPATRVFEA
                     AGAGACLITDAWEGIEMFLEPDKEVLVARDGREVAEHLAAL"
BASE COUNT          721 a         1585 c         1544 g          810 t
ORIGIN      
        1 ctcgcatcgc caacggtcaa tcgccgatgg tgttcgagga cggtcagcaa cggcgtgatt
       61 tcgtgcacgt gtcggacgtc gcccgagcct tccttctggc tctcgacaag cctgaagccg
      121 acggcgaggt gttcaacatc ggatcgggcg aagaccgcac cgtggaagag gtcgcccgac
      181 tgcaggccgc gtccatgggc cgcccggaca ttgaacccag catcacacaa caggctcgcg
      241 ccggcgacat ccgccacaac atcccggacc tgtcgaaggc ccgcgacgtg ctcggctatg
      301 aagcgcggca ggacttcggc caagggctgg aggagctcgc cgaatgggtc gcccgacagg
      361 aagcccacga caatgtcgcc aaggcccgcc acgagctgga aacccgagga ctggtggcat
      421 gaccaaggag gcgggaggca ccctcctggt cacaggcggc gccggcttca tcggcagcaa
      481 tctggccgat cgactggcga gcgaggggca tcagatcatc gttctggacg ccctcacccg
      541 tccgggcgta gagcgcaatc tcgcctggct cgaggcgcgc catggctccc ggatcgagag
      601 gctggtggcg gacgttcgcg acgaggacac ggttcgcagc gccgtatccc gctcggatgc
      661 ggtcttccac atggcgggcc aggtcgcggt caccacaagc ctcgtcgacc cgatcgagga
      721 tttcgagatc aacctcaagg gcacgctgaa tgtcctggaa gccgcgcggc ccaagctgac
      781 gccggtgatc ttcgccagca ccaacaaggt ctacggcgac ctcgccgata tcgacctgca
      841 gctcgtggaa ggcgcctgga cccccagcga tccgcaggtg cgcgcccggg gtgtgagcga
      901 agcccggccg ctcgacttcc acacgccata cggctgttcc aagggggccg ccgaccagta
      961 cgtgctggac tacgcaagga gcttcggcgt gccggctgca gttctgcgca tgagctgcat
     1021 ctatgggccc cggcaaatgg gcaccgaaga tcagggctgg gtcgcccact tcctcatccg
     1081 ggcgctcgag ggacgcacga tcagcctcta cggagacggg aggcaggtcc gcgatatcct
     1141 gaacgtgcga gacgcggtcg acgcctatat cgcggcctgg cgaaacatcg gcagcgtcgc
     1201 tggtcgagct ttcaacctcg gcggcgggcc gtcgaacgcg gtcagtctgc gccagttgat
     1261 cgcgcacatc gaaggcctga tcgggcgcaa ggtggcggtc gactacagcg actggcgcgc
     1321 aggcgatcag aagtatttcg tcgccgacgc ctcggccgcg cagtccgcgc ttgggcttca
     1381 gccgcccacc gcatggacag acgggatcgt gcagcttgcg gagtggctcg ccgaagagcg
     1441 tgggctttcg atcacgtcgc gcgaggtcgc agcatgaagc gcggcgcgcg cggccttcgc
     1501 gttctcctga ccgctgactc cgtcggcgga gtctggactt acgctctgga cctggccgcc
     1561 gggctagcag cgaaaggcgc ggaggtcatc atcgcgcata tgggcctgcc cccgaccgag
     1621 ccccctgccc tgtctgacgg cgtcgagatc gtcttcaccg gtctgcccct agattgggga
     1681 gatgttgacg ccgatgcgtt gagctcggcc gggcaggctt tggctgacct cactcgtgtg
     1741 cgcgacgtgg atctggtgca cctgaacagc cctattctgg cggcggacgt cgcctttcac
     1801 gtaccggtcg tcggcgggtg ccattcctgc gtcgcgacat ggtgggacgc cgttgagggt
     1861 catcgtccgc tgccggcgga cttcatctgg cgcaccgacc gtctgcgtgc ggggtatgcg
     1921 gcctgtgacg cgctcatcgc ccccacccgg tccttcgccg aagccaccac gggccgctac
     1981 ggtcagccgt gccaagtggt ctggaatggc cgcacgccac cctcgaccgg acctttgtca
     2041 ggccctcgtc ggcctgtcgc tgtcaccgcc ggtcgtctct gggatcccgc caagggcgcg
     2101 gcgactctgg acactgcagc cggaaaaatg cgcgcccggg tcgaagctgc cgggcctctg
     2161 gccaacccct tcgacccacg cccggtctct ttcgcccatc tgaacctgct cggtcggctg
     2221 agccccggcg aggtcgccga gcttttgtcg aacagtgcgg cgttcgtctc agccgcccgg
     2281 tacgagccgt ttggtctttc ggtccttgag gcggctcagg cgggctgcgc gctggtcctc
     2341 tcggatctgc cggtattccg cgagctttgg gacggggccg ccgtcttcgt cgatccgacc
     2401 gacaccagcg ctctggctga gacgttggat gcattgctgg acgatccagc ggagacccgg
     2461 cgtctgggcg agttggccca cacccgggcg gggcgcttca gcttggacgt catgctcgcc
     2521 gggacgctgg ccatctacgc cgatgttctg gaccgggcgg cccgtcccag cgaggcggcg
     2581 gcgtgaagat cgtctacttc acccattctc tggcttcgtg ctggaaccac ggaaacgctc
     2641 acttcctgcg cggggtgctc cgcgagctga aagcgctggg gcacgaggtc tctgcctggg
     2701 agccggaggg ctcctggagt ctccagaacc tgctgcagga cgccggaccg tccgggcttg
     2761 aggcctggcg caaggcctac ccggacctgt cctcccgcac cttcacggcg agcgcggagg
     2821 ccgagcgggg ggcctgcgac ggcgcggatc tggttatcgt acacgaatgg aacgatcccg
     2881 agctggtcgc cgccatcggt cgcgttcgcg caggcggcgc gcgcttcacc ctgctgtttc
     2941 atgacaccca ccaccgggcg gtcagcgagc cgaatgcgat ccacgccttc gatctgtcgg
     3001 gctatgacgg ggtgctggcg ttcggagaga ccctcgccca ggtttatcgc cgctggggct
     3061 ggggcgacca ggtgttcgtc tggcatgagg ccgcagatat ccgcctgttc cacccgccga
     3121 ccgaggaatg cgcccgcgag gggctggtct ggatcggcaa ctggggtgaa ggtgaacgca
     3181 ccgccgaact tgagtccttt ctgctcgccg ccgccgagga tgccggcctg ccgctggaca
     3241 tctatggcgt caggtacccg accgaggcgc ttagcctgct gaagcgctac ggggccaact
     3301 acaaggggtg gatcgccaac gcgtccgccc cggccgtctt cgcccgccat cttgcgacag
     3361 tgcatgtgcc ccggcgcttc tatgtcgagc agttgccagg cattcctacg atccgcgtgt
     3421 tcgaggctct ggcctgcggc attccactgg tcagcgcgcc ctggcgagac accgagaacc
     3481 tgttccggcc cggccaggac ttcctcgtgg cagccgacgg agaggccatg gccggtcatc
     3541 ttcggaacct gcgcgatgac gccgggctgc ggcagtcggt tgtcgccggc ggtctggaga
     3601 cgatccgcgc ccgtcacacc tgcgctcatc gggctcagga acttctcgcc gtcgccgacc
     3661 gcctcggcgt cgcccatgcc gacccttcac ccgttctgga gaccgtccga tgaagatcgc
     3721 cttctatggc tcgagcctcc tgtcgtccta ctggaacggc gccgcgacct attatcgagg
     3781 catgctttcc gagctgtcca agctgggctg gcaggtgacc ttctacgaac ctgacgcctt
     3841 cgaccgccag cagcaccgcg acatcgagcc gccatcctgg gccgaggtcg tcgtctatga
     3901 agccacgccc gaagcttgcg gggcggtcat ggcgcgggcg gcggaagccg acgtggtcgt
     3961 caaggctagt ggagtgggcg tcttcgacaa cgaactgctc ggcgtcatgg gcttcgcgcg
     4021 gcccgacgcc ctccgcatct tctgggacgt ggacgctccc gcgactctgg ccgagttgcg
     4081 ccagaacccg gatcatccgc tgaaggccgc gctacccacc ctcgatcatg tgttcaccta
     4141 tggcggcgga ccgccggtga tcgccgccta cctcgatata ggggcgcgag attgccggcc
     4201 catctacaac gccctcgatc ccacgaccca tcatccgacg gcgcccgacg accggttccg
     4261 cgtcgatctg gctctgttgg ccaaccggct gccggatcgt gagcgccgga tcgagcacta
     4321 cttcctggaa cctgccgccc tcctgccgga tcggactttt ttgctcggcg ggagcggctg
     4381 ggaggacaag gccatgccgg cgaacgtccg tcgtctgggg catgtcggca ccgccgagca
     4441 caacgccttc aacgtttcac ccctggctgt tctgaacgtc gcccgggaca gtatggccga
     4501 ggtcggtttc tcacccgcca cccgggtgtt cgaggctgcg ggagcaggcg cttgcctcat
     4561 caccgacgcc tgggagggca tcgagatgtt cctggagccg gacaaggaag tgctcgtggc
     4621 ccgcgatggc cgcgaggttg ctgagcacct ggccgccctg
//
LOCUS       DASVTM010000436         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_97466, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000436 DASVTM010000000
VERSION     DASVTM010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97466"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VF633_11945"
     CDS             <1..285
                     /locus_tag="VF633_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase"
                     /protein_id="HEX8471815.1"
                     /translation="IEENYGSQSVKLPAGDMVLYPSKSLHRVTPVTRGARVSSFMWLQ
                     SLVRDDGARETLFQLDVATQRVNLEKGPQDQAVIELTGVYHNLLRRWAEV"
     gene            complement(485..1846)
                     /locus_tag="VF633_11950"
     CDS             complement(485..1846)
                     /locus_tag="VF633_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEX8471816.1"
                     /translation="MSNDPVHWIAAIAAVVIWLIVCVSAAAISMRARRADRARLASFK
                     ADIGATVIAYASQTGFAEELAEMTARALTDAGEAARVVSFADLDIDMLASAKRALFVV
                     STTGEGDPPDSAGRVVRRMLDRDAALPDLSYGLLALGDRSYKDYCGFGHAVDGWLMRA
                     GATPLFDVVEVDDADPEAIRHWQHQLNALTGSNAEPDWTPPSYDRWRLVERRLLNPGS
                     PGGEAFHLAFEPIGHTPDWFAGDIAEVGVPASGGDRPAAREYSIASLPSEGRLELLIR
                     LMQHPDGTPGLASGWLTRRAEVGDEIAMRVRVNRGFHGPEAATPLILIGNGTGLAGLR
                     AHLKTRAVGNAGTWLMFGERTSAHDAFHDAELQAWLASGALRRLDRTFSRDAGDGRYV
                     QAVIAEQAEEVRAWVARGAAVYVCGSLEGMAEGVHAALEEVLGADGLIALAEAGRYRR
                     DVY"
     gene            complement(1848..>2371)
                     /locus_tag="VF633_11955"
     CDS             complement(1848..>2371)
                     /locus_tag="VF633_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX8471817.1"
                     /translation="DFSGIAKGHAVDRVSERLTAMGATSHLIEIGGELRGAGVKPDGQ
                     PWWVEIEQAPGSPAPRTLIALIDIAMATSGDYRRVFSHDGQRYSHTLDGRTGRPIDNG
                     LASVTVVHPSAMKADAFATALSVMGAADGREFAEDYGLAAHFVERTPQGAVEHMTPLF
                     AAMLEDQDEQQDV"
BASE COUNT          419 a          867 c          721 g          364 t
ORIGIN      
        1 atcgaggaga actacggctc ccagtcggtc aagttgccgg ccggggatat ggtcctctat
       61 ccgtccaaga gcctgcaccg ggtcacgccg gtgacacgcg gcgcgcgggt gtccagcttc
      121 atgtggctgc agagcctcgt ccgcgacgac ggcgcccgcg aaaccctgtt ccagctcgac
      181 gtcgccaccc agcgcgtcaa ccttgaaaag ggcccgcaag atcaggccgt gatcgaactc
      241 accggcgtct accacaacct cctccgccgc tgggcggagg tgtgaaaagc tcatccctct
      301 cccggcggga gagggctgga ggctcgcaga gcgcagcgat gcgtcagccg aaagggtgag
      361 gggctggcgt ccgatgtaag gacacctctt ttcgagacgg ctcacaccga ctgcaccgcc
      421 aacccctcat ccgccagcct tcggctgcca ccttctcccg gcgggagaag gaaagatcag
      481 gagctcagta aacgtcccgc cgataccgcc cggcttccgc caacgcgatc aacccatcag
      541 cgccgagcac ttcctcaagc gccgcgtgca ctccctcagc catgccttca agcgagccgc
      601 agacgtagac ggccgctccg cgagcgaccc acgcgcgcac ttcctcagcc tgttcagcga
      661 tcaccgcctg cacataccgc ccatccccgg catcgcgcga gaatgtccgg tccaaccgcc
      721 tcaatgcccc cgacgccagc cacgcctgca gctccgcatc gtggaacgcg tcgtgagccg
      781 atgtccgctc cccgaacatc agccaggtcc cggcattgcc gaccgcccgc gtcttcaaat
      841 gcgcccgcag cccggccagc cctgtaccgt taccgatcag gatcagcggc gtcgccgcct
      901 ccggcccatg gaagccccgg ttgacccgga cccgcatggc gatctcgtcg ccaacctcag
      961 ctcgtcgcgt cagccagccc gaggccagcc ccggcgtccc gtcggggtgt tgcatcagcc
     1021 ggatcaacag ctccaaccgt ccctccgaag gcagggacgc gatcgaatac tcacgcgccg
     1081 ccggacggtc gcctccgctg gccggcacgc cgacctcggc gatatccccg gcgaaccagt
     1141 cgggcgtgtg accgatcggc tcgaacgcca gatggaaggc ctcgcccccc gggctgcccg
     1201 ggttcagcag gcggcgctcg accaggcgcc agcggtcata cgacggcggg gtccagtcgg
     1261 gttcggcgtt cgatcccgtc agggcgttca actgatgctg ccagtggcga atggcctcgg
     1321 gatcggcgtc atcgacctcg accacatcga acaggggcgt cgctccggcc cgcatcagcc
     1381 agccgtcgac cgcatggcca aagccgcaat aatccttgta gctccggtcg cccaaagcca
     1441 gcagcccata ggacaggtcg ggcagggccg cgtcccgatc cagcatccgg cgcacgacac
     1501 gtccggcgct gtcgggagga tcaccttctc cggtggtcga gaccacgaac agggcgcgct
     1561 ttgccgatgc cagcatatcg atgtcgagat ccgcgaagga caccacccgc gccgcttcac
     1621 ccgcatcagt cagggcgcgc gcagtcatct cggccagttc ctctgcgaac ccggtctggc
     1681 tggcgtaagc gataaccgtc gccccgatgt ccgccttgaa ggacgccagc ctcgcccggt
     1741 cagcccggcg agcgcgcatc gaaattgccg cggccgacac acagacgata agccagatga
     1801 cgactgccgc gatggccgcg atccagtgaa cgggatcgtt gctcacctca cacgtcctgc
     1861 tgctcgtctt ggtcttcgag catggcggcg aacagggggg tcatatgttc gaccgcgccc
     1921 tgcggcgtgc gttcgacgaa atgcgcggcc agaccgtaat cctcggcgaa ctcgcgtccg
     1981 tctgcagcgc ccatgacgct gagcgccgtt gcgaaagcgt cggccttcat cgccgaagga
     2041 tgcaccacgg tgacggaggc tagcccgttg tcgatcggcc gaccggtacg gccatccagc
     2101 gtgtgggaat agcgttggcc gtcatggctg aagacccggc ggtaatcgcc tgaggtcgcc
     2161 atggcaatgt cgatcagggc gatcagtgtg cgaggcgccg gacttcccgg cgcctgttcg
     2221 atctcgaccc accacggttg gccgtccggc ttgaccccgg cgccgcggag ttcgccacca
     2281 atctcgatca ggtgcgaggt cgcccccatc gccgtcaggc gttcggagac tcggtccacc
     2341 gcatggccct tggcaatgcc cgagaaatcc a
//
LOCUS       DASVTM010000437         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_947650,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000437 DASVTM010000000
VERSION     DASVTM010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947650"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..218
                     /gene="thiS"
                     /locus_tag="VF633_11960"
     CDS             21..218
                     /gene="thiS"
                     /locus_tag="VF633_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX8471818.1"
                     /translation="MRIQVNGDTREVASRTILELVGELGLDPRKVAVERNLEIVPKSL
                     HGETALADGDRIEMVVFVGGG"
     gene            262..1062
                     /locus_tag="VF633_11965"
     CDS             262..1062
                     /locus_tag="VF633_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX8471819.1"
                     /translation="MNAHAPIATDATDTWTVAGRTFSSRLIVGTGKYVDYAQNAAAAE
                     AAGAEIVTVALRRVNLSDPTQPMLVDHVKPDRFTFLPNTAGCFTGEDAVRTLRLAREA
                     GGWDLVKLEVLSNTAHLYPDMIETLRALDLLIKDGFDVMVYCTDDVVMAKRLEDAGAA
                     AIMPAAAPIGSGLGIQNPVNIRLIVEQARVPVLVDAGVGTPSDAVLAMELGCDAVLMN
                     TAIAGAKDPILMARAMKHAVMAGRQGYLAGRMPRRMYADPSSPLAGLI"
     gene            1135..2832
                     /locus_tag="VF633_11970"
     CDS             1135..2832
                     /locus_tag="VF633_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471820.1"
                     /translation="MRLKLLAFTVATLALIGPAAVADATADKAVLDALDNAPQCGKVG
                     SAMTTAPVADMVLVDGFGDEGYAVDANPEAQVWFNQGVRLRWAFEHTEAVRAFRKARA
                     LDPTCGMCAWGEAWALGPNLNGGGNDDESNAAALKAAREARRLARDATPMQKQMIDAL
                     IQRYSGRKATRAERFARGMERIVDRNPNDVAIAAITADALMLSIDGEPWKKDGTAAYP
                     ATTRTMQVLEHALAVKPNDPASIHLYIHMTEWSADPHKAIPYGEQLAVLAPNASHLVH
                     MPSHTYYRVGRYVDAMRSNVNAVALDKRYEQVAPRPGGIAGGMPLHGHNLHFGMGGAL
                     MAGGAEDGLALADGFLRTYPDMPASNAWRQLMANDAYALYGRFGTQEAVAALVEPPAT
                     NPLLRMSWRYARGEAAARRGDAAAVTAEAEAIDAINAGIPETAPQAKEIKSFVELFQK
                     VLEGRAAMISGDHAAAVAAYARAAAIQAQDDEGGDPPIIWYPTRRSLAAAMLANGDAA
                     GAKAKIEALLKDWPADPYSYFVLAEAEKALGNTAAMDEALRQSKIQWIGGKMDLGLA"
     gene            complement(2836..3642)
                     /locus_tag="VF633_11975"
     CDS             complement(2836..3642)
                     /locus_tag="VF633_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8471821.1"
                     /translation="MTDDAPATPPTPATAPEKPTLKSRWMTGRSMLALAVSVVALGFA
                     AAPYVVPGLFGSQVRAYLLSNPGVLDEAVAARQATEDSARVAAINANVAAHPELLRAD
                     PRDPSFGPADAKVTVIEFFDFRCPGCKTVAADYLRLMRAHPDVRFVFKDWPILDRSGP
                     PVSQMAARAAIAAHQQGKYLQVYEGLMAERALDQAAIERVLEANGVSLPAAEAVINAP
                     DTSRHLADIHTTAATFQLTGTPTFLINGKTTSSIAPEEVVKAIEAAKTGG"
     gene            complement(3662..>4323)
                     /locus_tag="VF633_11980"
     CDS             complement(3662..>4323)
                     /locus_tag="VF633_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8471822.1"
                     /translation="TLRTYLETDTSFPARYARAIAWYRDGQTQRALDATEALIAEDID
                     NPYLWELKGQILFEDGRPADSLEAHRKSIELKPDAPLLRINLAHALIETNDATNLDPA
                     IDQLKRAVAREPDNTLAWRLLSQAYATQGKEGQARLASAEYWFAAGDTDQATQFALRA
                     RPMLDRNSIEWRRAVDIVLASGATDQDIQDLDQRQQRNRGPIVEPASAATIPATASPA
                     S"
BASE COUNT          713 a         1357 c         1529 g          724 t
ORIGIN      
        1 cgaaaggctg acgtagcggt atgcgcattc aagtcaacgg agatacgcgc gaagtcgctt
       61 cccgcacgat tctggaactg gtcggtgaac tcggtctgga cccgcgcaag gtcgccgtcg
      121 aacgcaatct cgagatcgtg ccgaagtcgc tgcacggcga aaccgcgttg gcggacggcg
      181 atcgcatcga gatggtggtg ttcgtcggcg ggggctgaat cccgggtttc gtttcgatct
      241 ctgtgcgcct aaatcaagcc catgaacgcg cacgctccca tcgctaccga cgccactgac
      301 acctggaccg ttgcgggtcg gacgttttcc tcgcggctga tcgtcggcac cggcaaatac
      361 gtcgactatg cccagaacgc cgccgccgcc gaggctgcgg gcgcggagat cgtgaccgtg
      421 gccctgcgcc gggtgaacct cagcgatccg acgcagccga tgctggtcga tcacgtcaag
      481 ccggaccggt tcaccttcct gccaaatacc gccggctgtt tcaccggtga ggacgccgtg
      541 cggacgttgc ggctggcgcg cgaggccggc ggttgggatc tggtcaagct ggaggtgctg
      601 tcgaacaccg cccacctgta tcccgacatg atcgagacgc tgcgggcgct ggacttgctg
      661 atcaaggacg gcttcgacgt catggtctac tgcaccgacg acgtcgtcat ggccaagcgg
      721 ctggaagacg ccggggccgc cgccatcatg cccgccgccg cgccgatcgg ctcgggtctg
      781 ggcatccaga accccgtcaa catccgactc atcgtcgaac aggcgagggt tccggtgctg
      841 gtcgatgcgg gcgtcggcac gccgtcggac gcggttctgg cgatggagct gggctgcgac
      901 gcggtactga tgaacacggc catcgccggc gccaaggatc cgatcctgat ggccagggca
      961 atgaagcatg ccgtcatggc cggacgtcag ggctatttgg ccggacggat gccgcgccgg
     1021 atgtacgccg atccgtcgtc gccgcttgca ggcctgattt agcgaccctt ccgccgcatt
     1081 ggccttgagg ccggttttcg gccacactgt ctcccggctg ttcgggagat gaggatgcgt
     1141 ttgaagctgc tggctttcac cgtggcgacg ctggccctga tcgggcctgc cgccgttgcc
     1201 gacgcgactg ccgacaaggc ggttctcgac gccctcgata acgcccctca gtgcggcaag
     1261 gtcggctcgg ctatgaccac cgcgccggtc gccgacatgg tgctggtcga cgggttcggg
     1321 gacgagggct atgccgtcga cgccaatccc gaggctcagg tctggttcaa tcagggcgtc
     1381 cgcctgcgct gggccttcga acacaccgag gccgtgcgcg cgttccgcaa ggcgcgggcg
     1441 ctcgatccaa cctgtggcat gtgcgcctgg ggcgaggcct gggcgttggg accgaacctg
     1501 aacggcggtg gcaatgacga cgagtccaac gctgcggccc tgaaggcggc gcgcgaggcc
     1561 cgacggctgg cgcgtgatgc gacgccgatg cagaaacaga tgatcgacgc cctgatccag
     1621 cgctactcgg gccgtaaggc cacgcgagcc gagcggttcg cgcgcggcat ggagcggatc
     1681 gtggatcgta accctaacga tgtcgcgatc gcggccatca ccgccgacgc cctgatgctt
     1741 agcatcgacg gagaaccttg gaagaaggac ggcacggccg cttatccggc cacgacccgg
     1801 accatgcagg tgctggagca cgcgctggcg gttaagccga acgacccggc cagcatccac
     1861 ctctatatcc acatgaccga atggtcggcg gatccgcaca aggccattcc ctacggcgag
     1921 cagttggccg tgttggcgcc gaacgccagc catctggtcc atatgccgtc gcacacctat
     1981 taccgcgtcg gtcgctacgt cgacgccatg cggtcgaacg tgaacgccgt ggcgctggac
     2041 aagcgctatg agcaggtcgc gccgcgtccc ggcgggattg cgggcggcat gcctctgcat
     2101 ggccacaacc tgcatttcgg catgggcggg gcgctgatgg cgggcggggc cgaggatggg
     2161 ctggcgctgg ccgatggttt cctgcgtacc taccccgaca tgccggcgag caacgcctgg
     2221 cgccagctca tggccaacga cgcctatgca ctctacggtc gcttcggcac gcaggaggca
     2281 gtggccgcgc tggtcgagcc tccggcgacg aacccgctgc tgcggatgag ctggcgctat
     2341 gctcggggtg aggctgcggc gcgtcgcggc gacgcagcgg ccgtgaccgc cgaggccgag
     2401 gccatcgacg cgattaacgc cggtatccct gagactgccc cgcaggcaaa ggagatcaag
     2461 agcttcgtcg agctgttcca gaaggtgctg gagggcaggg cggccatgat ctcgggagac
     2521 cacgccgccg ccgtcgccgc ctatgcgcgc gcggccgcca ttcaggctca ggacgacgag
     2581 ggcggcgacc cgccgatcat ctggtacccg acccgccgca gcctcgccgc cgccatgttg
     2641 gccaacggcg acgccgccgg agcgaaggcg aagatcgagg ctctgctcaa ggactggcca
     2701 gccgaccctt acagctattt cgtgctcgcc gaggccgaga aggcgctggg gaacacggcc
     2761 gctatggatg aagccctgcg tcagtcgaag atccagtgga tcggcggcaa gatggatctg
     2821 ggtctggcgt aagccttagc cgccggtctt cgccgcctcg atggccttca ccacttcttc
     2881 tggcgcgata ctcgacgtcg tcttgccgtt gatcaggaag gtcggcgtgc cggtcagttg
     2941 gaaggtcgcg gcggtcgtgt ggatgtcggc caggtggcgg ctggtgtcgg gtgcgttgat
     3001 aacggcttcg gcggcgggca ggctgacgcc gttagcttcc agaacgcgtt cgatcgcggc
     3061 ttgatcgagg gcgcgttcgg ccatcaggcc ttcgtagacc tggaggtatt tgccctgctg
     3121 gtgagcagcg atggcggcgc gggcggccat ctgggagacg ggcgggccgc tgcggtcgag
     3181 gatgggccag tccttgaaga cgaaacgcac atccgggtgg gcgcgcatca agcggagata
     3241 gtcggcggcc accgtcttgc agcccgggca gcggaaatcg aagaactcga tcaccgtcac
     3301 cttggcgtca gccgggccga acgacggatc acgggggtcg gcgcgcaaaa gctctggatg
     3361 ggcggcgaca ttggcgttga tggcggcgac gcgggcgctg tcctcggtcg cctgacgcgc
     3421 tgcgacggcc tcgtcgagca cgccgggatt gctcagcaga taggcgcgca cctgactgcc
     3481 gaacaggccg ggaacgacat agggcgcggc tgcgaaaccc agcgcgacga cggacaccgc
     3541 cagcgccaac atgctccggc cggtcatcca acgcgacttc agcgtcggct tctcaggtgc
     3601 ggtcgcaggc gtgggcgggg tcgcgggggc gtcgtcggtc atgaaggctc gaaacttgta
     3661 atcaggaggc gggggaggcg gttgcgggaa tggtcgcggc ggaggccggt tccacgatcg
     3721 gaccccggtt gcgctgctgg cgctggtcga gatcctggat gtcctgatcg gtcgcgcccg
     3781 aggccaggac gatatcgaca gcgcgacgcc attcgatcga gttgcgatcc agcatcggac
     3841 gggcgcgcag ggcgaactgg gtcgcctgat ccgtgtcacc ggcggcgaac cagtattcgg
     3901 ccgaggccag acgcgcctga ccctccttgc cttgggtcgc ataggcctgt gacagcaaac
     3961 gccaggccag cgtgttgtcc ggctcgcgcg ccacggcccg cttcaactgg tcaatggcag
     4021 ggtcaagatt ggtcgcgtcg ttggtctcga tcagggcgtg ggcgaggttg atgcggagca
     4081 ggggagcgtc cggtttcagc tcgatggatt tacggtgcgc ctccagcgaa tcggccgggc
     4141 gaccgtcctc gaacaggatc tggcccttga gttcccacag gtagggatta tcgatgtctt
     4201 cggcgatcag ggcctcggtg gcgtcgagcg cacgctgggt ctgaccgtcg cgataccagg
     4261 cgatggcgcg ggcgtagcgg gccgggaagg aggtgtcggt ctccagataa gtgcgcaatg
     4321 tct
//
LOCUS       DASVTM010000438         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_522865,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000438 DASVTM010000000
VERSION     DASVTM010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522865"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VF633_11985"
     CDS             complement(<1..269)
                     /locus_tag="VF633_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8471823.1"
                     /translation="MPIATRAFAATSATTPLAPYNFTRRDPLPDDVAIDIKFAGICHS
                     DLHMVHNDLGGSRYPLVPGHEIAGLVTAVGSNVTKFKVGDRVGVG"
     gene            complement(578..1327)
                     /locus_tag="VF633_11990"
     CDS             complement(578..1327)
                     /locus_tag="VF633_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8471824.1"
                     /translation="MTGAIKVGVGGWTYEPWRGAFYPDKLAQKQELAYMASKLTSIEI
                     NGTYYSGFKIDTWMKWRDETPADFVFSVKASRFCTNRKVLSEGGESFARFLDQGLTAL
                     GDKLGPINWQFMATKKFDPEDFEGFLKLLPKEKDGVRLRHALEVRSPTFDTEQFRDLA
                     RKYGAAIVYAEDDEEPNWPKIDEPTADFTYARLMSSREDEATGMSSEDLDRIAKQTAG
                     WAERGDVFAYFIAGAKVRNPAAAQALIEKLK"
     gene            1829..>3777
                     /locus_tag="VF633_11995"
     CDS             1829..>3777
                     /locus_tag="VF633_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471825.1"
                     /translation="MNATSRLRAFCMLGGSLGALMFATSVNAQTTPEPQAEDATAVDE
                     IVVTGFRASLANALNIKRREAGVVDAISAEDIADFPDTNLAESIQRIPGVSIDRDAGE
                     GRSITVRGLGPDFTRTRINGIESQATTGATDSSGGTNRGRGFDFNVFASELFNNITVR
                     KTSSAEVEEGSLGATVDLRTSRPFDYQGFTAAASIQDGYNDLSKTWSPRFAVLASNTA
                     FDGQIGALFSLAYSEKDSMEEGFSSVRWGPANGDGGFQNPTALPGGTTAASGIYHPRI
                     PRYGRLTHEQERTGATFSFQFKPNHGKTLLTFDSLYSKLDSTRRENFLEAWSFSRAAA
                     QGGKPEVDIVNTEVDPNTGEMVYAVFNDVDLRTENRFDVLETQFTQSTLNLEQKFTDS
                     FRFSGAIGRSKSEFANPLQVNAIIDRQNVAGYSWDFRENRDLPLINYGFDVANPNAWG
                     FTGPAAAQPRSELRLSSSYQTNIITTAEGAFEWDASEVFTFKAGASRKEYSSNGTGFS
                     RLNGVNDAPALPAGTSMASVTQLLTGFGRNLDLPAGSATSWIIPDLEALRTLFKWDCN
                     CNTGVPGGDFRLLGLESTASLASWREVTETDTGYWVQADWDTSLFGVPFRGNVGVRQV
                     KTEVESLGYSAIGGVAVAASSTNEY"
BASE COUNT          708 a         1226 c         1152 g          691 t
ORIGIN      
        1 cagccgacgc cgacgcggtc gccgaccttg aacttcgtca cgttcgatcc gaccgccgtg
       61 accagacctg ctatttcgtg gccggggacc agcgggtacc gcgagccgcc aaggtcgttg
      121 tgaaccatat gcaggtcgga atggcagatg ccagcgaact tgatgtcgat ggcaacgtcg
      181 tccggcaggg gatcgcgccg cgtgaagttg taaggcgcga gaggcgtggt ggccgaggtc
      241 gcggcgaagg cgcgggtggc gatgggcatg gtcgaaatcc tgtggcgagc gcggccaatg
      301 gcgggtcgag tgtcttcagt cgcgcgataa aaagcggcgg gatccatgca tcgatttttt
      361 ctgacgaccg aagcctcgcg tacgaccggg cgggcctcgt tcagagatca tatcgacggc
      421 gtggagcccc ggacgagggc tggacggtgg ttccagccct ccaactgaat gagggggctc
      481 gaacggcccg gcgcaagggg ccgaacggat tgatttgagg gtcgaatgtc tccacccggg
      541 ggtcaaatgt ctccagagta tcagggccga gagtctccta tttgagcttc tcgatcaggg
      601 cctgcgccgc cgctggattg cggaccttcg cgccggctat gaaataggcg aacacgtcgc
      661 cgcgttcagc ccagcctgcg gtctgtttgg cgatccggtc gaggtcctcc gagctcattc
      721 cggtcgcctc gtcttcgcgg ctcgacatca gccgggcgta ggtaaagtcg gcggtcggct
      781 cgtcgatctt cggccagttc ggctcttcgt catcctccgc atagacgatg gctgcgccgt
      841 atttgcgggc cagatcgcgg aactgctcgg tgtcgaaggt cgggctgcga acctccagcg
      901 cgtgcctcag acgcacgccg tccttctcct tgggcaacag cttgaggaac ccctcgaaat
      961 cctcgggatc gaatttcttg gtggccatga actgccagtt gatcggcccg agcttgtcgc
     1021 ccagcgccgt cagcccctga tcgagaaacc gcgcgaagct ctcgccgccc tccgacagga
     1081 ccttgcggtt ggtgcagaag cggctggcct tgaccgaaaa cacgaagtcg gcgggcgtct
     1141 cgtcgcgcca cttcatccag gtgtcgatct tgaaccccga atagtaggtc ccgttgatct
     1201 cgatcgaggt cagcttgctc gccatatagg caagctcctg cttctgggcg agtttgtccg
     1261 gatagaacgc accccgccaa ggctcgtacg tccacccgcc cacaccgacc ttgatggcgc
     1321 ccgtcattcg attctcctgc tgccgccttg atcgcaggat ttagagacgc tcgctaccga
     1381 aagccgcttc gcgcggggtc ggggaatggc tggaaacgga cctcgcgtca tgcaaatcga
     1441 gggatcgcca cgacgttcag gcgagccggc ctcatgtcgc aggcacattg cgtccgccta
     1501 ctcgcaggtc ccggcgccag cgcctgcgcc ggcgttggac gttggacgtc tcgacgttcc
     1561 attggtcaag gtaatggcaa accgttccac gttttgtgcg gctgtggcgg aagcgcgccc
     1621 aagccttgaa ctgacgcttc tcgtgccgcg cgacggcgcc aaaccagaac gcaatctggc
     1681 gagtatcgcc ttgttgttgc cttgatgcgg ggtccgtgcc agagttgtga caccggtatc
     1741 aaaaccgatt ggcatcagcc tgtttgggaa gaacggcccg aaagagaccg cagagtctcg
     1801 acgggtctca gggaagggaa ccccatctat gaacgcgacc tcgcgcttgc gcgctttctg
     1861 tatgctgggc ggatcgctcg gcgcattgat gttcgccacc tcggtgaacg ctcaaacgac
     1921 tccggaaccc caggctgaag acgcgacggc ggtcgacgaa atcgtcgtca ccggcttccg
     1981 cgccagtctg gcgaacgcgc tcaacatcaa acgccgcgaa gccggcgtcg tcgacgctat
     2041 ctcggccgag gacatagccg acttcccgga caccaacctg gcggaatcca tccagcggat
     2101 cccgggtgtc tcgatcgacc gtgacgccgg tgaaggccgc tcgatcaccg tgcggggcct
     2161 cggtcctgac ttcacccgta cccgcatcaa cggcatcgag tcgcaagcga ccaccggcgc
     2221 caccgactcg tcgggcggca ccaaccgcgg ccgcggcttc gacttcaacg tcttcgcctc
     2281 ggaactcttc aacaacatca cggtccgcaa gacctcttcg gcagaggtcg aggaaggatc
     2341 gctgggggcg acggtcgatc tgcgcaccag ccggccgttc gattatcaag gcttcaccgc
     2401 agccgcctcg atccaggacg gctacaacga cctgtccaag acctggagcc cgcgctttgc
     2461 cgtgttggcg tccaacaccg cattcgacgg ccagatcggc gccctgttct cgctggccta
     2521 ctccgaaaaa gactcgatgg aagagggatt cagctcagta cgctggggcc cggccaacgg
     2581 cgacggcggc ttccagaatc ccacggcctt gcccggcgga accactgctg cgagcggcat
     2641 ctaccatccg cgcatcccgc gctatggccg cctgacccac gaacaggagc gcacgggcgc
     2701 gaccttctcg ttccagttca agcctaacca cggcaagacc ctgctgacct tcgacagcct
     2761 ctattccaaa ctggattcaa cccggcgcga gaatttcctc gaagcatggt cctttagccg
     2821 cgccgcagcg cagggcggca agcccgaggt tgatatcgtc aacaccgagg tcgatccaaa
     2881 caccggcgag atggtttatg ccgtgttcaa cgacgtcgat ctgcgaacgg aaaaccggtt
     2941 cgacgtactt gaaacccagt tcacccagag caccctgaac ctcgagcaga aattcaccga
     3001 cagcttccgg ttctccggcg cgatcggtcg ctcgaagtcg gagttcgcca acccgctaca
     3061 ggtcaacgcc atcatcgatc ggcagaacgt ggccggctac agctgggatt tccgcgagaa
     3121 ccgtgacctg ccgctgatca actacggctt cgacgtcgcc aatcccaacg cctggggttt
     3181 caccggaccg gctgccgccc agccccggtc ggagttgcgt ctgagctcca gctaccagac
     3241 caacatcatc accacggctg aaggtgcgtt cgagtgggac gcctcggaag tcttcacgtt
     3301 caaggccggc gccagccgca aggaatacag ctccaacggc acaggcttct cgcgactgaa
     3361 cggcgtcaac gacgccccgg ccctgcctgc cggaacgtcc atggcgtcgg taacccagct
     3421 tttgacgggt ttcggtcgca acctcgacct gccggcaggt tcggccacct catggatcat
     3481 ccccgatctg gaagcgctcc gcacgctgtt caaatgggat tgcaactgca acaccggcgt
     3541 accgggcggc gatttccgcc tgctgggtct ggaaagcacc gcctctctcg ccagctggcg
     3601 tgaggtcacg gaaacggaca ccggttactg ggtacaggcc gactgggaca cgtccctgtt
     3661 tggtgtgccg ttccgaggca atgtcggcgt tcgccaggtc aagaccgagg tcgaatcgct
     3721 gggttactcc gccatcggag gggtggcggt cgccgcatcc agcacgaatg agtacga
//
LOCUS       DASVTM010000439         4560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_891964,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000439 DASVTM010000000
VERSION     DASVTM010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891964"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF633_12000"
     CDS             complement(<1..306)
                     /locus_tag="VF633_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8471826.1"
                     /translation="MGDQDDERKYRAPALEKGLDVLELLSAEGEAMTPSQMSITLGRS
                     VSELFRMIQVLEFRGYIEQSSDGYRLTNRLFSLGMTQAPIKSLVDVAMPAMRDLTFAT
                     "
     gene            494..2068
                     /locus_tag="VF633_12005"
     CDS             494..2068
                     /locus_tag="VF633_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HEX8471827.1"
                     /translation="MTLATIDLVILAIYGVCIFGLAQWVSRDRGGARKTSTDYFLASK
                     NLPWWAIGASLIAANISAEQIIGMSGSGFRIGLAIASYEWMAALTLLIVGKFFLPIFL
                     RNDIVTMPQFLQQRYGPTIRNVMAVFWLLLYVFVNVTSILWLGSIAVMTVTGLDQNYA
                     ILAIGAFALLYQLWGGLKAVALTDIVQVALLVAGGLIIGFIALSNIGDGAGPIAGFTT
                     LMTEFPEKFDMILSKDNPHYKELPGIAVLLGGLWVMNISYWGFNQYIIQRALAAKSIG
                     EAQKGIAFAAYLKLLMPVIIVLPGLAALVLAPGLAKPDQAYPTMMNLLPVGLKGLVFA
                     ALIAAIVASLASKVNSISTIFTLDLYAKARKGMSEQHLVLVGRIAAVVAMIIGVLVAR
                     PLLGGFSQAFQFIQDFTGFFTPSIVVIFILGLFWKRASEAGALTAAIGGVALSGILFW
                     LDKAGVFTLPFMNRVGLVFVVAVIAAIVVSLLVPPKPGTMQIRLDGVSYKTSIGFNIA
                     AVGVILFLIAVYTIWW"
     gene            2070..2975
                     /locus_tag="VF633_12010"
     CDS             2070..2975
                     /locus_tag="VF633_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX8471828.1"
                     /translation="MSSTASGDTALIGVDWGTSNLRVMRIDASGAVLESRSDPRGAAS
                     LTPDQFPDVLAAVAGAWIEQEAPVIVCGMAGARGRWLETAYAPCPASLADLGAALVRP
                     EGARNVAIIPGVAVFDEGGLSDVMRGEETQAMGLFGPGEDGLVLAPGTHSKWIKIEDG
                     RIAAFRTFVTGELFAAIKGATVIGAGLGDAGRDEAAFLEGVRRSLADHALTAALFSVR
                     VQRLSGRLSDGEAADYLSGLLIGAEIAAQSSLARDARVVIVGAPALAERYRLAMDAGG
                     FTRIVVADGQAATATGLWRIWSAAQ"
     gene            2972..3613
                     /locus_tag="VF633_12015"
     CDS             2972..3613
                     /locus_tag="VF633_12015"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX8471829.1"
                     /translation="MIDALLSELDALPLVAILRGLQPDEAVEIGEALVAAGFRCLEVP
                     LNSPDPLVSVRRLRDALDGRAVVGAGTVLTADAVRAVADAGSQVVISPNTDVDVIRAA
                     KAAGLLSMPGFFTPSEAFAALGAGADVLKLFPAELAGPAGLRAVRAVLPRTTRVYAVG
                     GANPASLGEWRDAGASGFGIGSSLYKPGRTAAETAFAARNFVAAWNGIETNAS"
     gene            4220..>4560
                     /locus_tag="VF633_12020"
     CDS             4220..>4560
                     /locus_tag="VF633_12020"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8471830.1"
                     /translation="MKMKSLLLAFAPVVLVSAFSSAKAAEAENKPEAGEVVQTQTQIP
                     TTRPPVTANTEAVFSTGVARGRDRLDSATSTSALRQNEIQALGARSLADIIRNIPGIR
                     TESSTGDGNSA"
BASE COUNT          753 a         1475 c         1418 g          914 t
ORIGIN      
        1 tgtggcgaag gtcagatccc gcatcgccgg catagcgacg tcgaccagag acttgatagg
       61 cgcctgggtc atcccgaggc tgaacaaccg gttggtgagc cggtagccat ccgaggactg
      121 ttcgatatag ccccgaaact ccaacacctg gatcatccgg aacagttcgc tcacggatcg
      181 tccaagagtg atcgacatct gggacggcgt catggcttcg ccttccgcag acaggagctc
      241 gaggacgtcg agacccttct cgagcgccgg ggcgcgatat tttcgctcgt cgtcctgatc
      301 gcccattcga ttccttccac gccgaaactc aagaatagcg agcccgccca tccaccgaaa
      361 gcagggttcc tacgtatccg tcgttatcgc ccgtaaaatg ttagcgcaca tataaaaaca
      421 gattgcgact tgatgacgcg tcgcctacgg ttttggccga gcccgcaaag ggccgagctc
      481 cgggaggctc ctcatgacgc ttgcgaccat cgatctcgtc attctcgcca tctatggcgt
      541 ctgcatcttt ggtctggcgc agtgggtttc acgagaccgg ggcggggcca ggaagacctc
      601 taccgactat ttcctcgcct ccaagaacct tccctggtgg gccatcggcg cctccttgat
      661 cgccgccaac atctcggccg aacagatcat cggcatgtcg gggtccggtt tccgcatcgg
      721 tctggccatc gcttcctacg aatggatggc cgcgctgacg ctgttgatcg tgggcaagtt
      781 cttcctgccc atcttcctgc gcaacgacat cgtcaccatg ccgcagttcc tgcagcagcg
      841 ttacggccca accatccgta acgtcatggc cgtgttctgg ctgctgctct acgtcttcgt
      901 caacgtcact tcaattctct ggcttggctc catcgcggtt atgaccgtga ccgggctcga
      961 ccagaactac gccattctgg cgatcggcgc attcgccctg ctgtatcagc tgtggggcgg
     1021 gctcaaggcc gtagccctaa ccgacatcgt gcaagtcgcc ctgctggtcg ccggcggcct
     1081 gatcatcggc ttcatcgccc tgtccaacat cggcgacggc gcgggcccga ttgcgggctt
     1141 cacaaccctg atgaccgagt tccctgagaa gttcgacatg atcctgtcga aggacaatcc
     1201 gcactacaaa gaactgccgg ggatcgccgt cctgctgggc gggttgtggg tcatgaacat
     1261 cagctattgg ggtttcaacc agtacatcat ccagcgggcg ctggccgcca aatcgatcgg
     1321 cgaggcccag aagggtatcg cgttcgccgc ctatctgaag ctgctgatgc cggtcatcat
     1381 cgtcctgcca ggcctcgcgg ccctcgtgct ggcgccgggt cttgccaagc ctgaccaagc
     1441 ctatcccacg atgatgaacc tgctgcccgt cggcctgaag ggtctcgtgt tcgcggccct
     1501 gattgcggcg atcgtcgcct ccctggcgtc caaggtgaac tcgatctcga ccatcttcac
     1561 gctcgatctc tacgccaagg cccgcaaagg tatgagcgag cagcatctgg tcctcgtcgg
     1621 ccggatcgcc gccgtcgtcg ccatgatcat cggcgttctc gtggcccgcc cgctgctggg
     1681 cggcttcagc caggccttcc agttcattca ggatttcacc ggcttcttca ccccctccat
     1741 cgtggtcatc ttcatcctgg gcctgttctg gaagcgagcg tccgaagcgg gcgccctgac
     1801 tgcggccatc ggcggtgtcg ccctctcggg tatcctcttc tggctcgaca aggcgggcgt
     1861 cttcaccctg ccgttcatga accgtgtcgg cctcgtcttc gtcgtcgccg tgatcgcggc
     1921 gatcgtcgtc tccctgctgg tcccccccaa gccgggaacg atgcaaatcc ggctggatgg
     1981 cgtcagctac aagacctcga tcgggttcaa catcgcggct gtgggcgtga tcctcttcct
     2041 gatcgccgtc tatacgatct ggtggtgatg tgagttcgac cgcgtcaggc gacacggccc
     2101 ttatcggcgt cgactggggc accagcaacc tgcgggtgat gcggatcgac gcgagcggcg
     2161 cggtgctcga aagccgaagc gacccgcgcg gcgccgctag cttgacgcca gaccagtttc
     2221 cagacgtgct ggctgccgtc gcgggggcat ggatcgaaca ggaggcgccc gtcatcgtgt
     2281 gcgggatggc cggtgcgcgg ggcaggtggc ttgagaccgc ctacgcccct tgtcccgcct
     2341 cactggccga tctcggcgct gcccttgtcc ggcctgaagg tgcgaggaac gtcgctatca
     2401 tacccggcgt cgccgtcttc gatgaagggg gcctgtcgga tgtgatgcgc ggagaggaaa
     2461 cccaggcgat gggtcttttc ggccccggtg aggacgggct cgtcctcgcg ccaggaacgc
     2521 acagcaaatg gatcaagatc gaggacggca ggatcgcggc tttccggacc ttcgtcaccg
     2581 gcgagttgtt cgccgcgatc aagggggcca cggtaatcgg agccgggctt ggcgacgctg
     2641 gccgtgacga agcggctttc ctcgaagggg tgaggcgcag tcttgcggac cacgccctca
     2701 ccgccgccct gttttcggtt cgcgtccagc gtctgtccgg caggctttca gatggtgagg
     2761 cggctgacta tctctcaggc ctgctcatcg gggccgagat cgcggcgcaa tcttctcttg
     2821 cccgtgacgc gcgtgtggtc atcgtcggcg cgccggcgct tgccgaacgc tatcgtctgg
     2881 cgatggacgc cggcggcttc acccggatcg ttgtcgcgga tggccaggcc gcgacggcta
     2941 ctggcctgtg gcgaatatgg agtgctgccc aatgatcgac gccctgctgt ctgaactcga
     3001 cgcgctcccg ctcgtggcca ttctccgagg tcttcaaccc gacgaagccg tcgaaatcgg
     3061 cgaggccctc gtcgccgccg ggttccgttg cctcgaagtc ccgctcaact cgcccgaccc
     3121 gctggtcagc gtccgcaggc tgcgggacgc gctagacggg cgagcggtgg taggggcggg
     3181 caccgtcctg acggccgatg cagtgcgtgc cgtcgccgat gcgggcagtc aggtggtgat
     3241 ctctcccaac accgacgtcg atgtcattcg cgcggccaag gcggctggcc tgctgtcgat
     3301 gcccggtttc ttcacgcctt cagaagcctt cgccgctctc ggcgccggcg ctgacgtcct
     3361 caagctgttt cccgccgagc tcgccggtcc tgcggggctg cgcgccgttc gcgccgtcct
     3421 gccccgaacg acgcgggtct atgcagtcgg tggcgccaat cctgcctcgc tgggcgaatg
     3481 gcgcgacgcc ggagcgtctg gattcggcat cgggtcttcg ctctacaagc cgggccgcac
     3541 cgccgccgaa acagcctttg ccgcccggaa ttttgttgca gcctggaacg gcatcgagac
     3601 caatgcgagt tgatggcggc acgcagcgta aacggcggct atgagaagat ccggcgctgg
     3661 atgatcacgt tggggttcgt cgcttaacgg gtaggtggcc agtccggttg cggccacggc
     3721 gtcctgatga atggtctgcg gggtcgaggt ttccgccttc cggtattgca cgttgacggg
     3781 atggacccgc cgccaggtca ttctgcggag gtcctcgtga ccagggcgct cagcccgagg
     3841 tcatcgtcac cgcccggctg gctcctaggt ggccgccgtg gagcctcggc ctgaagtcgc
     3901 tccacagccg ggggcggctc cgcgaaaaga accggctcga gaaccctcat ttgccgatcc
     3961 gaatccgggc gcgccggatg caattgttca gatcacaggc gcaacgattc ccgagtggcc
     4021 gccccgtggt cacaacaccc tcgatgccca ctgacgtcac aagagcggga gcacgcttcg
     4081 agcacctctc aagggtctta agtatttata cgtagtactt aagttggtct gagttctgac
     4141 gggcccgggt agagttaatc tgcaattata cttcttggcc aatgtaagtt ggacacggtt
     4201 cgtaatccgg ggggaaacca tgaagatgaa atcattgctg ttggcgtttg caccggttgt
     4261 cctggtgtca gcgttcagct cggcgaaggc agccgaggcg gaaaacaagc ccgaagccgg
     4321 tgaagtggtc cagacgcaga cgcaaatccc gaccacccga cctccggtta ccgcgaatac
     4381 cgaggcggtt ttctccaccg gcgtcgcgcg gggacgcgac aggctggata gtgcgacctc
     4441 gaccagcgcc ttgaggcaga acgagattca ggcgctcggc gcccggtcgc tggccgatat
     4501 tatccgtaac atcccgggca ttcgcaccga gtcgtccacc ggcgacggca actcggccta
//
LOCUS       DASVTM010000440         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_891999,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000440 DASVTM010000000
VERSION     DASVTM010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891999"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF633_12025"
     CDS             <1..216
                     /locus_tag="VF633_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471831.1"
                     /translation="ASAIAAGLTPRKARLFWDRLVLWFGAGWAAAACAGVIAGVTLTP
                     HLTADDQTDAVLYQSSLAGVDDTEVLG"
     gene            213..749
                     /locus_tag="VF633_12030"
     CDS             213..749
                     /locus_tag="VF633_12030"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX8471832.1"
                     /translation="MTLLDSPRTLKVALAISVALNLFAVAGGVTVWVAKTKTEDKIQA
                     SQAPGREASFHEIVRDMDPAVQDRVRISFRASAMAAKPDFEQSRNARRQAIELSKAES
                     FDPVVISTLLDQSRAAEIRGRTRLERDTIALLATLDPKDRQSLSVIFNRRGKGGGDGG
                     RGRSDQARSGPNEPRPNP"
     gene            complement(750..3122)
                     /locus_tag="VF633_12035"
     CDS             complement(750..3122)
                     /locus_tag="VF633_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8471833.1"
                     /translation="MRVLMLALTLVIAAYVLLISRQTAKPEQEAEALRVVTLQQEARL
                     FAAETAGRIDVANTALIVAANALSRATNRPLDAAEAARAAAPGIGFAVLSSKGAVQAV
                     SGAPAESFIEGQTRTAGAFSALRLDAGRRALLTGKTVGTMRVVARIPLPTRTRARANQ
                     TLFLSTPADGVAAAQSGTAPPRFDHAVLKAAAGPVWARVDGGETVSLVAAPVGSTGLL
                     AVAVSPEEAGGGLLGDLWVLAAPIGLGLLVGLLMIAQGWRQRRVGKEWAESEHRFRVA
                     VEAARCGVWEWDLAAEEVTLSDYMATLLGLSDGGVVPASTVLDRVHPKYRELVEHALR
                     QAAAFGAFETTFPVTGANNTVRWIDARGQARGERDEVGFTNILGVALDITEARRAKAQ
                     AQAAEGRLRDGIESVSDAFSLFDRHGRLILCNQAFSDAFGLAPEAVRRGAMKDELNRI
                     AALSIRTDHPSTDLGAGMREVEMHDGRWLRLAERYTSDGGTVVTATDLTLIKRQEAET
                     QRAADSLRLMVDQLESSQEKLSLLARKYEIAMTRAEAANQAKSEFLANMSHELRTPLN
                     AINGFSEIMAGEMFGPVGDARYKGYAADILQSGQHLLSLINDILDMAKIEAGKMTLHY
                     EKVSLKEVCEDAIRLMRGKAEESGLVLVLEADGLPDIDADQRGMKQVMLNLISNAVKF
                     TPEGGAITVSVSKHGPGRQRVAVADTGIGIAAEDLGRLARPFEQVEGQHSKTTQGTGL
                     GLALTKSLIEMHRGLMSIDSEPGAGTVVSFEVPEHRPVEAEPHRREARAA"
BASE COUNT          576 a         1099 c         1067 g          597 t
ORIGIN      
        1 gcctcggcga tcgccgccgg cctgactccg cgaaaggcgc gcttgttctg ggatcggctg
       61 gtgctgtggt tcggcgccgg ctgggcggct gcggcctgcg ccggggtgat cgccggtgta
      121 acgctgacac cgcacctgac cgctgacgat cagaccgacg cagtcctcta ccaatccagt
      181 ttggccggcg tggacgatac cgaggtgctg ggttgaccct tctggactcg cctcgcacgc
      241 tgaaggtggc cctggccata tcagtcgcct tgaatctgtt tgcagtcgcc gggggcgtga
      301 cggtctgggt cgccaaaacc aagaccgaag acaagattca ggcctctcag gctccggggc
      361 gagaggcatc cttccacgag atcgtcaggg acatggatcc cgccgtgcaa gaccgggttc
      421 gcatctcatt cagggcctcg gcgatggcgg cgaagcctga cttcgagcaa tctcgcaacg
      481 cccgacgtca agccatcgaa ctcagcaagg cggagagctt cgacccggtc gtcatcagca
      541 ccctgcttga ccagtcacgc gccgccgaaa ttcgtggccg aacccgactg gagcgcgaca
      601 ccatcgccct gctggcgacg cttgacccca aggaccgcca gtctctgagc gtcattttca
      661 accgtcgcgg taaaggcggc ggagatggcg gccgcggtag gtctgaccag gcaagatcgg
      721 gaccgaacga accccggccg aacccataat caggcggccc gggcctcacg acggtggggc
      781 tctgcctcta ccggccgatg ctctggaacc tcgaagctca ccacagtgcc ggcgccgggt
      841 tcgctgtcga tagacatcaa gccccgatgc atttcgatca gcgacttggt cagggcgagg
      901 cccagccctg tgccctgggt ggttttggaa tgttggcctt caacctgctc gaacgggcgg
      961 gcgagacgcc ccaggtcctc ggcggcgatg ccgatgccgg tatcggcgac ggcgactctc
     1021 tgacgtccgg gaccatgttt ggagacggaa acggtgatcg cgccgccttc gggcgtgaat
     1081 ttcacggcgt tcgaaatcag gttgagcatg acctgcttca tacctcgctg atcggcgtcg
     1141 atgtcaggca gtccatctgc ttccagcacc agcaccagtc ccgattcctc agccttgccg
     1201 cgcatcaggc ggatggcgtc ctcgcagacc tctttcagcg acaccttctc gtagtgaagc
     1261 gtcatcttgc ccgcctcgat cttggccatg tcgagaatgt cattgatgag gctcagcaaa
     1321 tgctggcccg attgaaggat gtcggcggca taccccttgt agcgggcgtc gccgaccggc
     1381 ccgaacatct cgccggccat gatttcagag aagccgttga tagcgttcag cggggtgcgc
     1441 agctcgtggg acatattggc caggaactcg gacttggcct ggttggcggc ctcggcccgg
     1501 gtcatggcga tctcgtattt tcgcgccagc aggctcaact tttcctggct cgactccagt
     1561 tggtcgacca tcaggcgcag tgagtccgct gcccgctgcg tctccgcttc ctgacgcttg
     1621 atcagagtca ggtcggtcgc ggtgacgacg gtgccgccat cggatgtgta acgttccgca
     1681 agccgcaacc agcggccgtc gtgcatctcc acctcacgca ttcccgcacc gagatccgtg
     1741 gacggatgat cggtccttat cgacaaggcg gcgatgcgat tgagttcgtc cttcatcgcg
     1801 cccctgcgca ccgcctcggg ggccagaccg aaagcatctg aaaaggcctg gttgcaaagg
     1861 atcagtcggc catggcggtc gaacagcgag aaggcgtcgg atacgctctc gatgccgtca
     1921 cgcagacgac cctctgcagc ctgggcctgc gccttggcgc gccgcgcctc ggtaatatcg
     1981 agcgcgaccc ccagaatgtt ggtgaagccg acttcatcgc gttcgccgcg tgcctgaccc
     2041 cgggcgtcga tccagcgaac ggtattgttc gcgccggtga cggggaaggt cgtctcgaac
     2101 gcaccgaaag ctgcagcctg ccgcagggcg tgttccacca gctcccgata tttcggatga
     2161 acccgatcca gcacggtcga cgccgggacg acaccgccgt cggacaggcc gaggagcgtc
     2221 gccatatagt cggacagggt gacttcctca gcggcgagat cccattccca aacgccgcag
     2281 cgcgcagcct ctacggcgac gcggaagcga tgttcgctct ccgcccattc cttgcccacc
     2341 cgcctttgac gccaaccctg ggcaatcatc agcaagccga ccagcaaacc cagccctatg
     2401 ggcgcagcca gaacccaaag gtcgccgagc agcccgccgc ccgcctcttc agggctgacc
     2461 gcaacagcca gcaggcccgt cgaccctaca ggggctgcga cgaggctgac ggtctcgccg
     2521 ccgtccaccc tcgcccagac cgggccggcc gcggccttca ggaccgcgtg gtcgaagcgg
     2581 gggggcgcgg ttcctgattg cgccgcagct acgccatcgg cgggcgtaga aagaaacagt
     2641 gtctggtttg cccgggcgcg agtgcgcgtc ggcagcggga tgcgagcgac tacacgcatc
     2701 gttcccaccg tcttgcccgt cagcaaggct cgacggccag cgtccagacg aagggcggag
     2761 aacgctcctg cggtccgtgt ctggccttcg atgaaacttt ccgcgggggc tccactgacg
     2821 gcctgcactg cgcccttcga ggacaggacg gcgaacccga tccccggcgc agcggcgcga
     2881 gcggcctcgg ctgcgtccag ggggcggttg gtggcgcgcg agagggcgtt tgcggcgacg
     2941 atcagcgcgg tgttggccac gtcgatcctt ccggctgttt cagcggcgaa aagtctggct
     3001 tcctgctgca gggtgaccac gcgcagggct tcggcctcct gttcgggctt tgccgtctgg
     3061 cgcgagatca ggagcacata ggcggcgatg acgagcgtca gggcgagcat cagtactcgc
     3121 atccggctgg cgccttgtcc ggcgcccaga tcggacgatc tgcggccttg tccctgaatg
     3181 cggcgctcac tgccccgcaa ggcgctctcc cggactgaaa tgcttgcgct taccgaagat
     3241 cggcaagggc tgggcgattc gacgaagtct ggaatctagg ctgcgtcgcg gaatcctgtc
     3301 gaggtttggt taaccaaacg tatcaggcag cttgtgcac
//
LOCUS       DASVTM010000441         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_466758,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000441 DASVTM010000000
VERSION     DASVTM010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466758"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VF633_12040"
     CDS             <1..1108
                     /locus_tag="VF633_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8471834.1"
                     /translation="WSLILEYSLVVSAVAVGWSGYAVGFLNGLDIGGISLALPHALSV
                     GPHAGGIINLPAVFVIAVVAGLLLLGTRESANLNVVLVVIKIVALIVFVAIALPAFNA
                     SNFHPFMPNGFGAPFVKTGVMAAAAIIFFAFYGFDAISTAAEESKRPERDLAIGIVGS
                     MLICTALYMIVAAAAIGARPTASFAASPEPLALILRELGQGAAAKWIGASAVVALPTV
                     LLAFLFGQSRIFLGMARDGLLPARLAKISSRGVPAVVTIFTAVIVAILAGLLPLDELA
                     SLANAGTLAAFCAVGVCLVVLRLREPGRKRLFRVPLWPLVAAITVIGCIVFFLSLKPI
                     TQGGFLIWNAIGLVIYFVWSARNARLAKHPGEAA"
     gene            1108..1461
                     /locus_tag="VF633_12045"
     CDS             1108..1461
                     /locus_tag="VF633_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8471835.1"
                     /translation="MSRRDLSGAVDFSVLDRMTGGDDAISEEVLGLFTEQAAMWSAML
                     EPKDHGWRDAVHTIRGAAAGIGAGELADVCASAEVSDNALAAPALDRVKTALDIALSD
                     VAAWRHELMLRSLRG"
     gene            complement(1458..>1749)
                     /locus_tag="VF633_12050"
     CDS             complement(1458..>1749)
                     /locus_tag="VF633_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX8471836.1"
                     /translation="PAECTSMAEVRQGVDALDRALVALLAERQRYMDAAARIKPDRDA
                     VHDDARIEDVVAKVLVSAEQHHLSPTIAEPVWRLLIDRSIAYEFSVYDRTRS"
BASE COUNT          232 a          567 c          604 g          346 t
ORIGIN      
        1 ctggagcctg atcctcgaat attcgctggt cgtttcggcc gtggccgtgg gttggtcggg
       61 ctatgcggtc ggtttcctga acggtctgga tatcggcggg atcagcctcg ccctgccgca
      121 cgccctgtcg gtcggtccgc atgcgggcgg gatcatcaat ctcccggcgg tcttcgtcat
      181 cgccgtcgtc gccggcctgc tgctgctggg cacgcgtgag agcgccaacc tcaacgtcgt
      241 gctggtggtg atcaagatcg tcgccttgat cgtgttcgtg gccatcgcgc tgccggcgtt
      301 caacgcctct aacttccacc ctttcatgcc caacggcttc ggcgcgccgt tcgtgaagac
      361 gggggtcatg gcggcggcgg ccatcatctt cttcgccttc tatggcttcg acgccatctc
      421 gacggcggcc gaggaatcca agcgccccga gcgcgatctg gccatcggca tcgtcggctc
      481 gatgttgatc tgcacggcgc tgtacatgat cgtcgccgca gcggcgatcg gcgcccgacc
      541 gaccgccagc ttcgccgcaa gtcccgagcc cctggccctg atcctgcggg agctcgggca
      601 gggcgcggcg gccaaatgga tcggcgcctc ggccgtggtg gccttgccga ccgtgttgct
      661 ggctttcctg ttcggtcaga gccgcatttt cctcggcatg gctcgtgatg gtctgctgcc
      721 ggcccgtctg gcgaagattt cctcgcgcgg cgtgccggcg gtggtgacca ttttcaccgc
      781 cgtcatcgtc gccatccttg ccggtctgct gccgctggat gaactggcgt cgctggccaa
      841 tgccgggacc ttggcggcct tctgcgccgt gggggtctgc ctcgtggtgc ttcgactgcg
      901 cgagccgggc cgcaaacgct tgttcagggt tccgctgtgg ccgctggtcg ccgcgatcac
      961 cgtgatcggc tgcatcgtct tcttcctcag cctcaagccc atcacacagg gcggcttcct
     1021 gatctggaac gcgattggtc tggtgatcta tttcgtctgg tcggccagga acgcccggct
     1081 ggccaaacac cccggcgagg ccgcctgatg tcgaggcggg acctctcggg cgcggtcgat
     1141 ttttcggtgc tggatcggat gaccggcgga gacgatgcaa tctccgaaga ggtcctcggc
     1201 ctgttcaccg aacaggcggc catgtggtcg gccatgctgg agcccaagga ccacggctgg
     1261 cgtgacgccg tccacacgat ccgtggcgca gcggcgggga tcggagcggg cgaactcgcc
     1321 gacgtctgcg cctcggccga ggtgagcgac aacgctttgg ccgctcccgc gctcgaccgg
     1381 gtcaagaccg cgctcgacat cgccctgagc gacgttgcgg cctggcggca cgagctgatg
     1441 ctcaggagtt tgaggggcta gctgcgcgtc cggtcgtaga ccgaaaactc ataggcgatg
     1501 ctgcggtcga tcagcaggcg ccagacgggt tcggcgatcg tgggcgacag gtggtgctgt
     1561 tcggcggaga ccagaacctt ggcgaccacg tcctcgatgc gggcgtcgtc gtgaacggcg
     1621 tcacggtcgg gcttgatgcg ggcagcggcg tccatgtagc gctggcgttc ggccagtagg
     1681 gcgaccagcg cgcggtccag agcgtcgacg ccctgacgga cctcggccat ggaggtgcat
     1741 tcggcggga
//
LOCUS       DASVTM010000442         7142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_43019, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000442 DASVTM010000000
VERSION     DASVTM010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43019"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="VF633_12055"
     CDS             <1..1808
                     /locus_tag="VF633_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX8471837.1"
                     /translation="GVAVHARGADGPTLRPARYGDFLVLVRRRDATFEEIIRALKTEG
                     VPVAGADRLKLSSHIVFDDLIALGRFVLYPDDDLSLAEILRSPLCDVTDFGDAHSLYP
                     LADTEARGGRKLWAELRRRAHEQPEWARARDLLQAALDDRSRDPFAFFSGVLNRVDAT
                     GLSGRGRILARLGREAEEAIDETLAQVLAAEERGGTDLETCLSLLEAADVEVKREMEG
                     ARNEVRVMTVHGAKGLEAPVVILPDTTSRAKPQGPTLMPALLNANDPQAGEGWLMCPG
                     RAGDDCEASGQARQARVDRTDAETLRLLYVALTRARDRLIVMGRAVKRPDTGFEEKSW
                     WSVIAETFGRLAEARPDSVSEITIGAIEGVLRFGIDPGSVPLAVASTTETTAVPDWAR
                     TVPPAEPGARLSAPSKMDETLRIPAPSPLAATGGLGRFRRGDLIHRLLERLPEIDAVD
                     RPDAAVRMLARERGLDDDQRAEMITAAFGVLNDDRFAPVFGAGSRAEVALTGSAPGLN
                     GVSISGRIDRLVITPERVLVVDYKSNRPAPENIEGADPAYVLQLAVYVAVLKRLYPDR
                     AVEAALVWTDGPRLMGVPSEMMDEALTATRSANSR"
     gene            1911..2246
                     /gene="trxA"
                     /locus_tag="VF633_12060"
     CDS             1911..2246
                     /gene="trxA"
                     /locus_tag="VF633_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX8471838.1"
                     /translation="MATVKVTDESFDADVLKSSIPVLVDFWAEWCGPCKQIGPALEQI
                     ADELGGQVKIAKVNIDDSPMTPSKLGVKGIPTLMLFRDGQLSSLKVGAMPKGKIVEWL
                     AESGIKADA"
     gene            complement(2317..3609)
                     /locus_tag="VF633_12065"
     CDS             complement(2317..3609)
                     /locus_tag="VF633_12065"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX8471839.1"
                     /translation="MDPISERLRLRHPQKIDLSLDRMRTLCAVLGNPQDHLPPVVHVA
                     GTNGKGSTVAFIRAMAEAAGLRVHAYTSPHLVRFNERIRLAGRLIEDAPLNRILDRIE
                     AVRGVEATVFESTTAAAFVAMAETPADLAIVEVGLGGSLDATNIIERPLLSVIAPVDL
                     DHAEFLGTSITGIAGEKAGILKAGARGIVARQSEEAMAVIEAVAASALNGRTPSPLTV
                     MGVDFDAWAERGGMVFQDQERFLDLPAPALAGAHQIDNAGLAVAAALELDLPEAAIAE
                     GLRTVVWPARMQRLSTGPYGERAKAADAELWLDGGHNPHAARALADAIAARQAKAPLP
                     LALIVGMLGNKDAAGFFEALKDSGAHVFTVAFEGAAAEPEALAAVARGHGLGAQPAPS
                     VEAALDRALRLGAGRVVICGSLYLAGEVLGAAPETWPV"
     gene            4153..4830
                     /locus_tag="VF633_12070"
     CDS             4153..4830
                     /locus_tag="VF633_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8471840.1"
                     /translation="MFDSWATNLRSSRAVADVSVSRDLTARRRLSMILKRLFDMICAL
                     GALMVLAPALIAIAIAVAIESPGPVLFRQRRTGQGGKVFRIMKFRTMTVTEDGDVIKH
                     ASKNDKRVTRVGGFLRETSLDELPQLFNILLGDMSVVGPRPHALAHDAHYGALLPRYV
                     DRFAVRPGLTGFAQVQGLRGEIHDIDCMARRIDADVHYAASWSFRSDLLLILRTFPML
                     LKRVNAY"
     gene            4920..5774
                     /locus_tag="VF633_12075"
     CDS             4920..5774
                     /locus_tag="VF633_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX8471841.1"
                     /translation="MANTDTPVWFITGCSTGFGRELATALIAKGYRVVATARNPDDVA
                     DLAEAGGDRVLALKLDVTDDAQASAAIKAAEARFGHIDVLVNNAGIGYFAAVEESEQD
                     EVRQMFEINFWGLSRMTTLALPGMRARKAGMIVNISSVGGLRAFPSVGYYNATKFAVE
                     GLSEALSGELEPLGIKVLIVEPSGFRTDWAGRSANEAKNPIADYTDTAGKRKGEIRGY
                     SGAQPGDPKRAAAAIIQAVEADKPPLRLLLGKAALQGGRGKIQMLAKDFDAWAEVTEG
                     ADFPKGEG"
     gene            5851..6273
                     /locus_tag="VF633_12080"
     CDS             5851..6273
                     /locus_tag="VF633_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEX8471842.1"
                     /translation="MSILGKIFGGIFHKKDAPATPTATQAPKPTPAPIPTPTAAPMPS
                     AAAPTATVAAEPVDVAGILDFKNSVRDEKLNWRTSIVDLMKLVGLESSLTERKELADE
                     LGYTGDKSDSASMNIWLHKQVLQKIVDNGGQLPRDLTP"
     gene            6322..>7142
                     /gene="dnaX"
                     /locus_tag="VF633_12085"
     CDS             6322..>7142
                     /gene="dnaX"
                     /locus_tag="VF633_12085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX8471843.1"
                     /translation="MSDVDPSLDGPPWEEDLPERDDNTDDMFGAPAAAPVAPPSAAAS
                     PPRDLAPIPVSAPVPVEGDGEAYKVLARKYRPRTFEDLIGQEAMVRTLTNAFATGRIA
                     HAFMLTGVRGVGKTTTARLLARALNNETDVIDRPSLALTAHGRHDAAIMAGQHMDVME
                     MDAASHTGVGDIRDILESVRYGPVEARYKVYVLDEVHMLSNQAFNALLKTLEEPPPHA
                     KFIFATTEIRKVPVTILSRCQRFDLRRVEPEILVEHLSRIAGKEGMKVEDDALAL"
BASE COUNT         1302 a         2319 c         2276 g         1245 t
ORIGIN      
        1 gcggcgtcgc cgtccacgcg cgcggcgccg acggcccgac ccttcgtcct gcccgctacg
       61 gtgacttcct cgtcctcgtc cgccgccgtg acgcgacgtt cgaggagatc atccgtgcac
      121 tgaagaccga gggcgtgccg gtcgccgggg ccgaccgact caagctttcc agccatatcg
      181 tcttcgacga cctgatcgcg ctggggaggt tcgtgcttta tcccgacgac gacctgtcct
      241 tggccgagat cctgcgcagc cccctgtgcg acgtcaccga tttcggcgat gcgcatagcc
      301 tctaccctct ggcggacacc gaagcgcgag gcgggcggaa gctctgggcc gaactgcgtc
      361 ggcgggctca cgaacagccg gagtgggctc gtgcacgcga tctgctgcag gcggcgctgg
      421 acgaccggtc acgcgatccg ttcgccttct tctcgggcgt tctgaatcgc gtggatgcaa
      481 cgggactgtc aggtcgtggc cgcattctgg ctcggctggg acgcgaggcg gaagaagcca
      541 tcgacgagac gctggctcag gtgctggcgg cggaagaacg tggcgggacc gatctggaga
      601 cctgtctgtc cctgctcgaa gccgccgacg tcgaggtcaa gcgcgagatg gaaggggcgc
      661 gcaacgaggt ccgcgtcatg accgtccatg gcgcgaaggg gctggaggcg ccggtagtca
      721 tcctgccgga caccacctcg cgcgccaagc cacagggacc gaccctgatg ccggctttac
      781 taaacgccaa cgatccgcag gctggagagg gctggctgat gtgtccgggc cgcgccggtg
      841 acgactgcga ggcctcgggt caggcgcgtc aggctcgcgt cgatcgcact gacgcggaaa
      901 cccttcggct gctttacgtg gccctgaccc gcgcccgcga tcgcttgatc gtcatgggcc
      961 gcgccgtgaa gcgccccgat accggtttcg aagaaaagag ctggtggagc gtgatcgccg
     1021 aaaccttcgg gcggctggct gaagcccggc ccgatagcgt gagcgagatc accatcggag
     1081 cgatcgaagg cgtgctgcgg ttcggcatcg atccgggatc ggttccgcta gccgtggcgt
     1141 ccaccactga gacaacagct gttcccgact gggcgcggac cgtcccaccg gcggaacccg
     1201 gcgcgcgtct gtcggcgccg tcgaagatgg atgagaccct gcgcattcct gcgccgtcgc
     1261 cgctggctgc gaccggcggg cttggccggt tccggcgcgg cgacctgatc caccggttgc
     1321 tggagcggct gcccgagatc gacgccgtcg accgtcccga cgccgctgtt cggatgctgg
     1381 cgcgcgagcg gggcctcgac gacgaccagc gcgccgagat gatcacagcc gccttcgggg
     1441 ttctgaatga cgatcggttc gcgcctgtgt tcggcgccgg ttcccgggct gaggtcgccc
     1501 tgaccgggtc tgcgcccgga ctgaacggcg tctcgatttc tggccgcatc gaccggctgg
     1561 tgattacgcc tgaacgcgtg ctggtcgtgg actacaagtc caaccgcccg gctcccgaga
     1621 acatcgaagg cgccgacccg gcctatgtcc tgcagttggc ggtctatgtc gctgtcctga
     1681 agcggctcta tccggatcgc gcggtcgagg ctgcgctggt ctggaccgac gggccacggc
     1741 tgatgggcgt tccttcagag atgatggacg aggcgctgac ggctacgaga tcggcgaaca
     1801 gccgttgaac tcgtcgggaa tcaccccaca tctgcaccaa cggccatccc tcggtcgatg
     1861 acatcaatcc gcgctcaggc aacgagcctt agcgcctcaa ggagcctctc atggcgactg
     1921 taaaagtgac cgacgaaagc tttgacgccg acgtcctgaa atccagcatc ccggtgctcg
     1981 tggacttctg ggccgaatgg tgcgggccgt gcaaacagat cggcccggcg ctggaacaga
     2041 tcgccgatga gctcggcggc caggtcaaga tcgccaaggt caacatcgac gacagcccga
     2101 tgaccccatc gaagctcggc gtgaagggca tcccgacgct gatgctgttc cgcgacggtc
     2161 aactgtcctc gttgaaggtc ggcgccatgc ccaaaggcaa gatcgtcgaa tggctggccg
     2221 aaagcggcat caaggccgac gcgtaagtct tcgacacctg ctgagttcag acgcccggtg
     2281 gtccttgcga ccgtcgggcg ttttcctgtc cggctgtcag accggccagg tttccggcgc
     2341 cgcgccgaga acctcgcctg ctaaataaag cgacccgcag atcaccaccc gtccggcgcc
     2401 gagcctgagt gcgcgatcca gcgcagcctc gaccgagggg gcgggctgcg cgcccaggcc
     2461 gtgaccgcgc gcaacggcag ccaaggcttc gggttcagca gcagcgcctt caaaggcgac
     2521 ggtgaagacg tgggcaccag agtctttcag agcctcgaaa aagccggccg cgtccttgtt
     2581 gccgagcatg ccgacaatca gcgcgagcgg tagaggagcc ttggcctgac gggcggcgat
     2641 ggcgtcggcc aaagcgcgcg cggcatgagg gttatgcccg ccatcgagcc atagctcagc
     2701 gtctgcagcc ttggcgcgct cgccgtaagg cccggtcgac aggcgctgca tacgagcggg
     2761 ccagacgacc gtgcgcaggc cctctgcgat cgcagcctca ggcaggtcga gctcaagagc
     2821 cgccgcaaca gccagtccgg cgttatcgat ttgatgcgct ccggccagag ccggcgcggg
     2881 caggtcgagg aaccgttcct gatcctgaaa caccatgccg ccacgctcgg cccaggcgtc
     2941 gaaatccacg cccatgactg tcaggggcga aggcgttcgc ccgttcaacg ccgaggcggc
     3001 cacggcctcg atcaccgcca tggcctcttc cgactgacgc gccacgatgc cccgggcgcc
     3061 ggctttcagg atgcccgcct tctcgccggc gatgcctgtg atcgaggtcc ccagaaactc
     3121 ggcatggtcc agatcgacgg gagcgatgac gctgagcagc ggccgttcga tgatgttggt
     3181 cgcgtccagt gacccgccca ggccgacctc gacgatggcg agatctgcgg gcgtctctgc
     3241 catggcgacg aaggcggcgg ctgtcgtgct ttcgaacaca gtcgcttcga cgccacgtac
     3301 ggcttcgatc cggtccagga ttcggttgag cggcgcatcc tcgatcagcc gtccggcgag
     3361 gcggatgcgc tcattgaacc tgacgaggtg gggggaggta taggcgtgaa cacgaaggcc
     3421 agccgcctcg gccatcgccc ggataaaggc gaccgtagag cccttgccgt tggtgcccgc
     3481 cacatggacg acgggcggaa ggtggtcctg tggattgccg agcaccgcgc aaagcgtgcg
     3541 catccggtcg agggagagat caatcttctg cgggtgtcgc agacgcaggc gctctgaaat
     3601 cgggtccatc caggatacta gcgcactcca cgctcagtag cccacgcttg agaggctgac
     3661 cggagacttg atcagccgaa aatcatcttg aagaccccaa accacacggc gaccgatccc
     3721 gcagcagcgc cagcataggc gaaaagcgga gaggttagcg aaatacgcgc aggccgggac
     3781 accgatcggg ccgggacggc gcctacggaa ttgaagagtt tttcgccgga cgcacgcatt
     3841 tgaaccactc cctgttcgac gaccatcctg cgcctcccac ctttagacat ccggaaaatg
     3901 tgtggttaag cagagggttc ctcctgttaa gctctcatgc tgcgccgcga ttgtttcaca
     3961 cctgtctcaa gattgggaca gtgggttcaa aaacgataga aatttccgca acttggttag
     4021 atgttctggt tgcgcatcaa tcacatatcg ttgttccttt cttctatcgg ggaatttacc
     4081 ttgcagtgca gcatttgacc gggcttaact ttagggtaac tgcatttcgc gttcgtcaga
     4141 ggagatcagg gcatgttcga cagttgggcg accaatctgc gctcgagccg ggccgtggcg
     4201 gatgtttctg tttcgcgcga tttgacggcg cgccgccggc tctcgatgat cctgaaacgt
     4261 ctcttcgata tgatctgcgc tctgggcgcg ctgatggttt tggccccagc tctgattgct
     4321 atcgccatcg ccgtcgctat cgaatctccc ggtcccgtcc tgttccgtca gcgccgcaca
     4381 ggccagggcg gcaaggtgtt caggatcatg aagttccgca ccatgacggt gaccgaagat
     4441 ggtgacgtca tcaaacacgc cagcaagaac gacaagcgcg tcacccgcgt cggcggcttc
     4501 ctgcgcgaaa cgagtcttga tgaactgcca cagcttttca atatccttct aggcgacatg
     4561 tccgtcgtgg ggccgcgccc tcacgccttg gcccatgacg cacactacgg cgccctgctg
     4621 ccgcgctatg tcgaccggtt cgcggtccgc cccggcctga cgggctttgc ccaggttcag
     4681 ggtctgcgcg gcgaaatcca cgacatcgac tgcatggccc gccggatcga cgccgacgtc
     4741 cactacgccg ccagctggtc cttccgcagc gacctgcttc ttatcctgcg caccttcccg
     4801 atgctgctga agcgcgtcaa cgcctactga ggcgaagaat gtccggcgtg acgctgtggc
     4861 gcgcgttgaa ctctcgggcg cagcaaccca actgaagtct cccgatcagg agactcccta
     4921 tggccaatac cgataccccc gtctggttca tcaccggttg ttcgaccggt ttcggtcgtg
     4981 agctggcgac ggcgctcatc gccaagggct atcgcgtggt cgccactgca cgcaatccgg
     5041 acgatgtcgc cgatctggca gaggcgggcg gcgatcgggt tctggccctg aaactcgacg
     5101 ttaccgacga cgctcaggcc agtgcagcga ttaaagcggc ggaggcccgg ttcggccata
     5161 tcgacgtcct cgttaacaac gcaggcatcg gctatttcgc ggccgtcgag gaatccgaac
     5221 aggacgaggt tcggcagatg ttcgagatca atttctgggg tctgagccgg atgacgacct
     5281 tggcgttgcc cgggatgcgg gcgcgcaagg ccgggatgat cgtcaatatc tcatcggtcg
     5341 gaggtctcag ggcctttcca tcggtgggct actacaacgc gacgaaattc gcggtcgaag
     5401 gcctgtccga ggcattgtcc ggcgagctcg agcctctcgg catcaaggtg ctgatcgtgg
     5461 agccgtccgg cttccgcacg gactgggccg gccgctccgc caacgaggcg aaaaatccaa
     5521 tcgccgatta caccgacaca gcaggcaagc gtaaaggcga gatcagaggc tattccggcg
     5581 cccagcctgg cgatcccaaa cgcgccgccg ccgccatcat ccaggccgtc gaggccgata
     5641 agccgccgct tcgcctgctg ctcggcaagg ccgcccttca gggtgggcgt ggcaagatcc
     5701 agatgctcgc caaggatttc gacgcctggg cagaggtcac cgagggcgcg gatttcccga
     5761 aaggcgaagg ctagccgctg ttcccgggtc ttggcgggaa ccgtagcgct ccgtgatcgc
     5821 tcctgtcgtt caacggcagg agcgaacctc atgagcattc tcggcaagat tttcggcggc
     5881 atctttcaca agaaggacgc acccgcgaca ccgacagcga cgcaggctcc aaagcccact
     5941 cccgcgccga tcccaactcc aacggcagct cccatgccgt ccgctgccgc gccgactgcg
     6001 accgtggccg ccgagccggt ggacgttgcg ggtatcctcg acttcaagaa cagcgtgcgt
     6061 gacgagaagc tcaactggcg cacctcgatc gtcgatctga tgaagctggt cggtctggag
     6121 agctcgctca ctgagcgtaa ggagctggct gacgagctgg gctacacggg cgacaagtcc
     6181 gacagtgcga gcatgaacat ctggctgcac aaacaggtgc tgcaaaagat cgtggacaat
     6241 ggcggccaac tgccccgcga tctgacgcct taagccaaag cggagtcgca tcgaccgtct
     6301 tactcgcggt aagacggtcg catgagcgat gtcgatccga gtcttgatgg tccgccgtgg
     6361 gaagaagacc tccccgagcg cgatgacaac accgacgaca tgttcggcgc gcctgccgct
     6421 gcaccggtag cgcccccgtc cgcagcggcc tcgccgcctc gtgaccttgc accaatcccg
     6481 gtcagtgccc ccgtccccgt cgagggagac ggcgaagcct ataaggtcct cgcgcgcaaa
     6541 tatcggccac gcacgttcga ggacctgatc ggtcaggagg cgatggttcg caccctgacc
     6601 aacgccttcg ccaccggccg gatcgcccac gccttcatgc tcactggcgt gcgaggagtc
     6661 ggcaagacca cgacggcccg actgctggcc cgcgccctga acaacgagac cgatgtcatc
     6721 gacaggccgt cactggccct gaccgcccat ggccgtcacg acgctgcgat catggccggc
     6781 caacatatgg acgtcatgga gatggacgcc gccagccaca ccggtgtcgg cgacatccgc
     6841 gatatcctcg agtcggttcg gtatggtccg gtcgaggccc gctacaaggt gtatgtgctg
     6901 gacgaagtgc acatgctgtc caatcaggcc ttcaacgccc tgctgaagac gctggaagag
     6961 ccgccgccgc acgccaaatt catcttcgcc accaccgaaa tccgcaaggt gccggtgacg
     7021 atcctgagcc gttgccagcg cttcgatctg cgccgcgtcg agcccgagat actggtggag
     7081 catctgtctc gcatcgcagg caaggagggg atgaaggtgg aggatgacgc cctcgccctg
     7141 at
//
LOCUS       DASVTM010000443         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_789391,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000443 DASVTM010000000
VERSION     DASVTM010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789391"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..893
                     /locus_tag="VF633_12090"
     CDS             63..893
                     /locus_tag="VF633_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX8471844.1"
                     /translation="MGQRIGQLDQQRTGLERQIASNVEQRRLIEEELAGMRSLAARGF
                     APENRVRALERTAAALDGDLGSLRAQVASTGEAVGQTRLEMLGVSTKMNEDVADQLRQ
                     AEVSLNELRPKLSELRSQIALSQIRAPASGQIVGLTAFTVGGVIASGQVLMDVVPDNA
                     TQVIVASVNPNDVDNLRLGLSTEVKFPGLRDRSTPILRGTVSRISPDSFTDEKTGQSH
                     YRVEVIIPPLELEKLGASARHIRPGMPVEVVVLLRKRTALQYLVEPLVRSLWRTGSEE
                     "
     gene            1056..>3052
                     /locus_tag="VF633_12095"
     CDS             1056..>3052
                     /locus_tag="VF633_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8471845.1"
                     /translation="MPTLSTLSDDAGFTSGGSATSVQFSEGSFSSPCPGCGSFHANFD
                     GSDFGPLAFLNGDDRGGSTTNGKPSLTVTGAGEQITRTNQSWATGLGTAASVTFAFRS
                     TAPSTMPADTTGFTRFTDVQITATLAALQAWSDVANITFTRIADGSGYSDSATMLFGD
                     YASGQTGAAAFAYLPGSRTATSASGDVWVNYSGNNVSPGLLNYGQHTLTHEIGHAIGM
                     SHPAAYNADEGVTITYGSDATYYEDSRQYTVMSYFSETNTGGNYRVNGTGANQYSAVP
                     LLDDIAAAQRLYGANMTTRTGDTVYGFNSNADRAWFSATSASTSLIFAIWDAGGTDTL
                     DFSGYNQAATIDLRQGAFSSVGGMIGNVAIALGAVIENAIGGAGSDRIFGNSADNRLY
                     GGGLGADVIDGGLGVDTAVLPGVRSSYTITWNGQIGTINATGNPQSTTVTNVEFLAFA
                     DQTIAAAPTGGLNVAGDTTNDTMNGTGFADTLSGAGGNDVIYGLGGNDTLAGGTGADQ
                     ISGGDGDDILVGGSGNDALDGGAGIDLADYLGAASGVAVNLATGLATGGAGSDSLASF
                     ENVRGTAFDDSLTGDGSANSIRGGGGADTIRGGDGNDIIYSGPGGLGGGAPDIVKAQA
                     TANATRAAAVSLDGGFDLLSRANVSASSSIPHATIVATSSG"
BASE COUNT          585 a          997 c          924 g          546 t
ORIGIN      
        1 tgcgacttca acgcttgcag ttcgcggccc ggcgaagtgg tcgatcgact gagaccggcg
       61 tcttgggtca gcgcatcgga cagctcgatc agcaaagaac gggtcttgag cgtcagatcg
      121 catccaacgt cgagcaacgg cgcctgatcg aggaagaact ggccggcatg aggagtcttg
      181 ccgcccgcgg attcgcgccc gagaaccgtg tgcgagcgct tgaacggact gccgcagccc
      241 tcgacggcga tctcggctcc ctgcgcgcac aggttgccag tacaggcgag gccgtcggcc
      301 agacccgcct ggagatgctg ggtgtctcga ccaagatgaa cgaagatgtc gccgatcaac
      361 tgcgtcaggc cgaggtatcg ttgaacgaac tgcgcccgaa actgtcggag ttacgtagcc
      421 agatcgcgct gtctcaaatc cgcgcacccg cctccggtca gatcgtagga ctgacggcct
      481 tcaccgtcgg cggcgtcatc gcttcgggac aggttctgat ggacgtcgtg ccagacaatg
      541 ctacccaggt gatcgtcgcc tccgtcaacc ccaacgacgt cgataatctc aggctcgggc
      601 tgtcgacgga ggtcaaattt cccgggctgc gtgatcgctc gacaccgatt ttgcggggca
      661 ccgtcagtcg tatttctccc gacagcttca cggacgaaaa aaccggccag agccactatc
      721 gcgtggaagt gatcatacca cctttggagc tggaaaaact gggcgcctcg gcccggcata
      781 tccgcccggg catgcctgtc gaggttgttg ttctactgcg gaagcgaacc gctcttcagt
      841 atctcgttga gcccttggtg cgcagtttgt ggcggacagg ctccgaggaa tagcgcttct
      901 cgcacgacga tctggggccc gaaccaccgt ctcgcgcgac ctctgttctc attgtttaac
      961 tgccggccgg ttagaacccc gcactaccct gacgcttgcc cttcgcgaag gcctcgttac
     1021 ggtggtgaaa cagtcagttc tacatcaggt ccatcatgcc caccttgtcc acgctgtccg
     1081 atgatgcagg gttcacaagc ggagggtcgg cgaccagcgt tcagttcagc gaaggcagct
     1141 tctcgtcgcc ctgccccggg tgcggcagct ttcatgcgaa cttcgacggg tcggacttcg
     1201 gcccactggc gttcctgaac ggcgatgacc ggggcggttc aacaacgaat ggcaagccat
     1261 cgctgacggt cactggcgca ggcgagcaaa tcacccgcac caaccagagc tgggctacgg
     1321 ggctgggcac ggctgcaagc gtgaccttcg cctttcgcag cacagccccg tcgaccatgc
     1381 ccgctgatac caccggtttt acgcgattta ccgatgtcca gatcactgcc acgttagctg
     1441 cgcttcaagc gtggtcagac gtagccaaca tcaccttcac ccggatcgcc gacggcagcg
     1501 gctactccga cagcgcgaca atgctgtttg gtgactacgc gtctgggcaa actggagccg
     1561 ccgcgttcgc ttatctgccc ggcagtcgaa ctgcgacctc ggcctctggt gatgtctggg
     1621 tgaactactc aggcaataac gtctcgccgg gcctgctgaa ctacgggcag cacaccctga
     1681 cccacgagat tggccacgcg atcggcatga gccaccctgc ggcctataat gcggacgaag
     1741 gggtcaccat cacatacggc tcagacgcca cttattacga agattcccgc cagtacacgg
     1801 tgatgagtta cttcagcgag accaacaccg gcggcaacta tcgcgtcaac ggtaccggcg
     1861 cgaaccagta ttccgccgtg cccctactgg acgatatcgc agccgcgcag cgcctgtatg
     1921 gagccaacat gaccacgcgg accggcgata cggtctacgg gttcaactcc aatgcggacc
     1981 gggcctggtt cagcgcgacc tcggcctcaa ccagtctgat cttcgccatc tgggacgctg
     2041 gcggcacaga caccctcgac ttctcaggct acaaccaggc cgcgacgatc gaccttcgcc
     2101 aaggcgcgtt ttcgagcgtt ggcggcatga taggcaatgt cgccattgcc ttgggcgccg
     2161 ttatcgagaa cgcgatcggt ggcgcgggat ccgatcgcat cttcggcaat tctgccgaca
     2221 accgcctgta cggcggcggc ttaggcgctg acgtcatcga cggcggcctc ggcgtcgaca
     2281 ccgccgtcct ccctggcgtg cgctcaagtt acacgattac ctggaacgga cagatcggaa
     2341 ccatcaacgc aaccggcaac ccccaaagca caacggtcac caacgtcgag ttcctcgcct
     2401 tcgccgacca gaccatcgcg gccgccccga ccggcggtct gaatgtggcc ggcgacacga
     2461 ccaacgacac catgaacggc accggcttcg cggataccct gtcgggcgcg ggcggcaacg
     2521 acgtcatcta cggcctgggc gggaacgata ccctggctgg gggcaccggc gccgatcaga
     2581 tttccggcgg tgacggtgac gacatcctgg tcggcgggtc cggcaatgac gcgctggacg
     2641 gtggagcagg catcgatctg gccgactacc tcggggcagc aagcggcgtg gccgtcaact
     2701 tggcgacagg actggcgacg ggcggcgctg gctccgacag cctggcctcg ttcgagaatg
     2761 tgcgcggcac tgcgtttgac gactccctaa ccggagatgg gagcgccaat tcgatccgcg
     2821 gcggcggcgg cgccgatacg atccgcggcg gcgacggaaa tgacatcatc tattccggtc
     2881 ccggcgggct aggcggtggc gcaccggata tcgtgaaggc tcaggctaca gcgaatgcca
     2941 cccgcgcggc cgccgtatcg ctcgatggcg gtttcgatct actgtcgcgc gcgaacgtgt
     3001 cggcttcttc ctccatccca cacgccacaa tcgtcgccac cagcagcgga aa
//
LOCUS       DASVTM010000444         4926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_892204,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000444 DASVTM010000000
VERSION     DASVTM010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892204"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VF633_12100"
     CDS             complement(<1..669)
                     /locus_tag="VF633_12100"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX8471846.1"
                     /translation="MTDALLTASPPTPPTPSPFHGLKVLWVKHWTDSLFSFAVERPED
                     FRFRSGEFVMIGLPGADGGKPVMRAYSIASPAWAEEIEFFSIKVDDGPLTSWLQKIVA
                     GDTVLMGKKPTGTLVLDALTGGERLWLIGTGTGLAPWLSVARDPETYSRFGQVVVCHT
                     VRNVADLAYRDFLTHGIHEDPLIGDEAKAQLTYYPTVTRERFETPGRITDRIKSGDLF
                     ADLGL"
     gene            786..3047
                     /locus_tag="VF633_12105"
     CDS             786..3047
                     /locus_tag="VF633_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX8471847.1"
                     /translation="MRTVLLASTLLMAVAGSAFAQTAAPPAADPAILTPERAFANPSL
                     GGPVAQGVSLSPDGALVAFLRARTDDVSVLDLWAAPTGAGEPFKLIDARALVPDAGEL
                     SEAEKARRERMRISQRGVVEYSWDTQGRYILAPLEGDIYLSSRADQSVKRLTNTPGDE
                     IDAKVSPKGTYVSYVRDQDLVIQDLATNAVTPVTTDGDGLITWATAEFIAQEEMDRDT
                     GYWWSPDEHYIALQRTDESGVDIVPRLDINATGSTVVEQRYPRAGRPNAVVDLYVHDV
                     TTGQRIKVDLGTNTDIYLGRVDWSADGKTLYVQRQQRDQQTLDLLAVDPATGASRVLV
                     TQTSKTWVDLTSDFHPLEDGGFIWSDESTGWRHLYLYDRNGRKVRAITSGLYPVKGLN
                     GVNEATGDVFFTASMRDRKELPVEQQLFRASLRRAMTPVEITPAGGWWGVSMNRTATA
                     YVGNYSDPATPPRSGLYSADATFVRWIEENKLDAGHPFYPYVSRLRTPEFGTLQSHGQ
                     TLVWRLTTPPGFDPSKKYPVVMQVYGGPSTGAGVQKSWQPITNQLLTEAGYIVFRLDN
                     RGEGDRSAAFRSAQYLHMGTVETEDQVLAANYLRTLPYVDASKIAAMGWSYGGFMSLN
                     MLTEPKMGLAAAAAGGSPTNWSLYDTHYTERFMSTPRANPDGYAGTDVLNRLDNLTGR
                     LLLMHGMADDNVVFENTTRVMDALQARSIPFELMVYPGQRHGVQGNERKLQQWRTYLD
                     FFDRTIGSRPPAE"
     gene            3132..4832
                     /locus_tag="VF633_12110"
     CDS             3132..4832
                     /locus_tag="VF633_12110"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8471848.1"
                     /translation="MPSTFHASRLVRAGTGILAAVALTAIGRTPVTAAVQVSLPPADE
                     HRCQALETLSPTERAGAVRLLSGQGWREGQSVPCIWTMVGNNVGANTSVCVVGANPNI
                     EVCAERFAIMPRSGEDPFHAVIRYAASPQFPGPDVDDSSGLVGTPILLKDNIETADMP
                     TTAGSLALANNASGRDAPLVSRLRHAGAIVIGKTNLSEWANIRSSNSISGWSAVGGLT
                     PNPYDPTRTACGSSSGSAVAVAVGLAPVAIGTETNGSITCPASVNGVVGFKPTVGLVS
                     RTHIVPISHSQDTAGPIATTVEDAAIVLTAIAGSDPEDVATAEADTRKTDYRAALDAN
                     SLNGARIGVLRYAKGYSAPTMAVFEQNLQVLRDAGAVLVDIDTGPDMRTIGGESFKVL
                     MYELKADLNAYLATTDATQVKTRTLADVIAFNAATPAETVLFGQELFELAETKGDLTT
                     QEYLDARETSFRLAGPEGIDKMMADNTVVALIAPTTSRAWTNARDDEDDAEGSTSTIA
                     AVAGYPHLTVPMGFDRGMPVGLSFIGGKWDDAKILSLGYAFEQKTHARRAPGQVVKAD
                     "
BASE COUNT          879 a         1690 c         1538 g          819 t
ORIGIN      
        1 aaggccgagg tcggcgaaca aatcgcctga cttgatccgg tcggtgatgc gtccgggtgt
       61 ctcgaaccgc tcgcgggtta cggtcggata ataggtcagc tgggccttgg cctcgtcgcc
      121 gatcagcggg tcctcgtgga tgccgtgggt aaggaagtcg cggtaggcga ggtcggcgac
      181 gttgcgcacc gtgtggcaga ctacgacctg cccaaagcgc gaataggtct ccgggtcccg
      241 ggccaccgac aaccacggcg ccaaccccgt gccggtcccg atcagccaca gccgctcgcc
      301 gccggtcagg gcgtccagca ccagcgtccc ggtcggcttc ttgcccatca gcaccgtgtc
      361 gccggcgacg atcttctgca gccacgaggt cagcggaccg tcgtcgacct tgatcgagaa
      421 gaactcgatc tcctcggccc aggccgggct ggcgatcgaa taggcgcgca tgacgggctt
      481 accaccgtct gcccccggca aaccgatcat cacgaactcg ccggagcgga agcggaagtc
      541 ctcgggccgt tcaacggcga acgagaacag gctgtcggtc cagtgcttga cccacagcac
      601 cttcaacccg tggaacgggc tcggcgtggg tggcgtgggg ggcgaggcgg tcaggagagc
      661 gtcggtcatt gcctgctgtt taggcggtca gacgcgcgtg cgaaaggatc gcaggcgccg
      721 catcctgcac tagcgaccga aaaaatctcg gtgacgcgga tagaatccat ggttagtccc
      781 atgccatgcg tacagttctt cttgcttcga cgttgctcat ggccgtcgcc ggttccgctt
      841 tcgcccagac ggcagcccct cccgcagccg atcctgccat cctgacgcca gagcgcgcgt
      901 tcgcgaaccc cagccttggt ggtccggtgg ctcagggtgt cagcctgtcg cccgacggcg
      961 cgttggtcgc gttcctgaga gcgcggaccg acgacgtttc ggtgctcgac ctgtgggccg
     1021 ccccgaccgg cgccggcgag ccattcaagc tgatcgacgc ccgcgctctg gtccccgatg
     1081 cgggcgaact gtccgaggct gagaaggccc ggcgcgagcg gatgcgcatc tctcaacgcg
     1141 gcgtggtcga atacagctgg gatacgcaag ggcgctacat cctcgccccg ctggaaggcg
     1201 acatctatct gtccagccga gccgatcaga gcgtgaagcg cctgaccaac acgccgggcg
     1261 acgagatcga cgccaaggtc agtcccaagg ggacgtatgt ctcctatgtc cgggatcagg
     1321 atctggtcat ccaggatctg gcgaccaacg ccgtaactcc cgtcactacc gacggcgacg
     1381 gcctgatcac atgggccacc gccgagttca tcgcccagga ggagatggac cgcgacaccg
     1441 gctactggtg gagccccgac gaacactaca tcgctctcca gcgcaccgac gaaagcggcg
     1501 tcgatatcgt cccgcgcctc gacatcaacg ccacgggctc gacggtggtg gagcagcgtt
     1561 atccccgcgc gggccgcccc aatgcggtgg tcgatctcta tgtccacgac gtcacgacgg
     1621 gtcagcggat caaggtcgat ctggggacca ataccgacat ctatctgggt cgtgtcgact
     1681 ggtcggccga cggcaagacc ctctacgtcc agcgccagca gcgcgaccag cagacgctcg
     1741 acctgctggc cgtcgatccg gcgaccggcg cctcgcgcgt gctcgtcacc cagaccagca
     1801 agacatgggt cgacctgacc agcgacttcc atccgcttga ggacggcggt ttcatctggt
     1861 cggatgaatc gaccggctgg cggcatctgt atctgtacga tcgcaacggc cggaaggttc
     1921 gcgcgatcac ctcgggcctc tatccggtca agggtctgaa cggcgtcaac gaggcgaccg
     1981 gcgatgtctt tttcaccgcc tcgatgcgcg accgcaagga actgccggtc gaacagcagc
     2041 tgttccgcgc cagcctgcgc cgcgccatga cgccggtcga gatcacaccg gcgggcggct
     2101 ggtggggtgt gtcgatgaac cggaccgcca cagcctatgt cggtaactat tccgatccgg
     2161 cgacgcctcc gcgctccggt ctctacagcg ccgacgcaac gtttgtgcgc tggatcgagg
     2221 agaacaagct cgacgccggc caccccttct atccctatgt ctcgcgcctg cggacgccag
     2281 aattcggcac gcttcagagc catggccaga cgctggtctg gcgcttaacc acgccgccgg
     2341 gtttcgatcc gtcgaagaaa tatcccgtcg tgatgcaggt ctacggcggt ccctcgaccg
     2401 gtgcaggcgt gcagaagtcg tggcagccga tcaccaatca actgctgact gaggccggct
     2461 acattgtttt ccgcctcgac aaccgggggg agggcgaccg ctccgccgcc ttccgttccg
     2521 cgcagtatct gcacatggga acggtcgaga ccgaggatca ggtgttggcg gccaactatc
     2581 tgagaacgtt gccttacgtc gatgcgtcga agatcgcggc gatgggctgg agctatggcg
     2641 ggttcatgag cctgaacatg ctgaccgagc cgaagatggg cctcgccgcc gcagccgccg
     2701 ggggctcgcc gaccaactgg tcgctgtacg acacccacta caccgaacgc ttcatgtcga
     2761 cgcctcgggc caaccccgac ggctatgccg ggaccgacgt gctgaaccgc ctcgacaatc
     2821 tgaccggtcg gttgctgctg atgcacggca tggccgacga caacgtcgtg ttcgagaaca
     2881 ccacccgggt catggacgcg ttgcaggcca ggagcatccc gttcgagctg atggtctatc
     2941 cgggccagcg tcacggcgtg cagggcaacg agcgaaaact ccagcaatgg cgcacctacc
     3001 tcgacttctt cgaccgcacg atcggctcgc gaccacctgc ggagtaggcg agcgggtggc
     3061 tcttgtgttt cggcccgatc cccactcaaa gtcacgcttc cttcctcgca cgacgtccgg
     3121 gacaacctcc gatgccttcg accttccatg cgtcccgtct cgtcagggcc ggaaccggta
     3181 ttctggcggc ggtggccctg acggcgatag gccgaacgcc ggtcaccgcc gccgtccagg
     3241 tttcactgcc gccggcggac gagcatcggt gtcaggccct cgagaccctg tctccgaccg
     3301 agcgggccgg cgcggttcgt cttttgtcgg ggcaggggtg gcgcgagggc cagtccgtgc
     3361 cctgtatctg gaccatggtt ggcaacaacg tcggcgccaa tacgtccgtc tgcgttgtag
     3421 gcgccaaccc gaacatcgag gtctgcgcag agcggttcgc gatcatgccg cgatcaggtg
     3481 aggacccctt ccatgcggtc atccgttatg ccgcgtcgcc gcagtttccc ggcccggacg
     3541 tcgacgactc cagcgggctg gtcggcacgc ccatcttgct gaaggataat atcgagacgg
     3601 ccgacatgcc gaccacggcg ggatcgctgg cgttggccaa caacgcttcc ggccgcgatg
     3661 cgccgctggt ctctagactg cggcatgccg gggccatcgt catcggcaag accaacctgt
     3721 cggaatgggc caacatccgc tcgtcgaact cgatcagcgg ttggagcgcg gtcgggggcc
     3781 tgacacccaa tccgtacgac ccgacgcgaa ccgcctgcgg ttcttcctcg ggtagcgcgg
     3841 tcgccgtcgc tgttggtctg gcgcccgtcg ccatcggcac cgagaccaac ggctcgatca
     3901 cctgtcccgc ttcggtcaac ggcgtcgtcg ggttcaagcc gaccgtgggt ctggtcagcc
     3961 gaacccatat cgtgcctatc agccactcac aggacaccgc cggacctatc gccacgaccg
     4021 tcgaggacgc cgcaatcgtc ctcaccgcta tcgctggatc ggaccccgaa gacgtcgcca
     4081 ccgccgaggc cgacacccgc aagaccgact atcgcgcggc gctcgacgcg aactcgctga
     4141 acggcgcccg catcggcgtt ctgcgctacg ccaagggcta ttccgcgccg acgatggcgg
     4201 tgttcgagca gaaccttcag gtcctgcgcg atgccggcgc cgtcctcgta gacatcgaca
     4261 ccggtcccga catgcgcacc atcggcgggg agtcgttcaa ggtgctgatg tacgagctca
     4321 aggccgacct caacgcctat ctggccacca ccgatgcgac gcaggtcaag acccgcacgc
     4381 tcgccgacgt catcgccttc aatgccgcga cccccgccga gaccgtcctg ttcggccagg
     4441 aactgttcga attggccgag accaagggcg acctgaccac ccaggaatac ctcgacgccc
     4501 gcgagacctc gttccgcctc gccggacccg aaggcatcga caagatgatg gccgacaaca
     4561 ccgtcgtcgc cctgatcgcc cccaccacct cgcgcgcctg gaccaacgcc cgcgacgacg
     4621 aagacgacgc cgagggttcg accagcacca tcgccgccgt cgccggctat ccgcacctga
     4681 cagtgccgat gggcttcgat cgcggcatgc cagtcggact cagcttcatc ggcggcaagt
     4741 gggatgacgc aaagatcctg tctctcggct acgccttcga acagaagacc catgcgcgcc
     4801 gcgccccggg gcaggtcgtc aaagccgact aggaaccggg cgctgacagt ggaaccccgg
     4861 caaaagcggg gttgttcggc tgtaatggaa aaacacatcg tccccatcgc cgtgacccct
     4921 gacggg
//
LOCUS       DASVTM010000445         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_948281,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000445 DASVTM010000000
VERSION     DASVTM010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948281"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..1066)
                     /locus_tag="VF633_12115"
     CDS             complement(263..1066)
                     /locus_tag="VF633_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8471849.1"
                     /translation="MLKGTQVWTVPNPVSARNYEVFVSLPASYETSPERRYPVLYVTD
                     ADYAFPIIRQIARRVNLEGPVIEEFILVGLSYSQGDSATVSRNRDYTPTANGPARSSS
                     MLHGEGPAYQTYLKQQAIPFIENRFRAHPARRVLLGHSYGGLLGAQILFTDPTLFQGY
                     VLGSPSFWFDARHIMTMEARYARTHSDLRADVFMYIGEYETPGPSRRNPNIDMVGDVR
                     SMERILKSRGYANLQVHSVVLTDEDHLTVAPVGFTRALMAVLPAERQQR"
     gene            1488..1997
                     /locus_tag="VF633_12120"
     CDS             1488..1997
                     /locus_tag="VF633_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471850.1"
                     /translation="MTEQAAVDPAAAAFVLSLYGVESGGTGEAVPENDWDLEAVSSAR
                     TAALFAEAKALTPAGYIGYPEGHPLVEFEEWGSLKLEDVVTTQHGSDRADVSLILAFT
                     QPNVRLNRTFNLVKEDGRWRLDDLKYDQSVQTAQSGAQSGPNAPIPGLLDGFHSFIAE
                     AKAHPFEDQ"
     gene            2046..3776
                     /locus_tag="VF633_12125"
     CDS             2046..3776
                     /locus_tag="VF633_12125"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEX8471851.1"
                     /translation="MRSRPSASVELSPLRAFAELLDRLSLTSSRNAKLVLLRDYLKAT
                     PDPDRGWALAALTGDLSFDAAKPAMIRRAVEARVDSVLFGWSYDYVGDLAETVALIWP
                     GNPERRPNREPELSEVVDALAIAKRTEVQGLIEGWLDALAPKGRWALLKLVTGALRVG
                     MSARLAKTAAAMIRPASIMEPPDPEGGETATVLDAVDASDIEEVWHAVAPPYADLFAW
                     LEGRAERPSPDAPGRFRPVMLSVAIDEAVDFPKLSPADYAAEWKWDGIRVQAVREAGV
                     AKLYSRTGDEISGAFPDVMAGLTFEGAIDGELLVWRDGVVAPFGDLQQRLNRKTVDAK
                     MMATYPAAVIAYDVMAENGEDLRTLSLRERRTRLETLVAGHEGDRLHLSPLVEYDSWE
                     GLGTLRADPPVGAAAEGLMLKRWDSAYVAGRPKGPWFKWKRDPHTIDAVMMYAQRGHG
                     KRSSFYSDFTFGVWTEEGPLTPVGKAYFGFTDDELKLLDKFVRDYTIERFGPVRSVRA
                     ERDFGLVLEIAFEGLNRSPRHKSGVAMRFPRISRIRWDKPAREADTLDSVMDLLDAIE
                     SGGGRVAKVV"
     gene            3963..4505
                     /locus_tag="VF633_12130"
     CDS             3963..4505
                     /locus_tag="VF633_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471852.1"
                     /translation="MFFSPRIPTGATIALAVWCAAASGRAEPQDEPTQTPREALALVA
                     GGDLVLHDAAMIKVFTNVAPRAVVSSPLHITGIVEGGLFQEGVFPIYLMGHEGCVLAS
                     APARPLTDWMTEGEVAFEATLVFSAPPGSWATIVFEQDVEYADAPEPLDVRTRVTIAG
                     AEDPEYLVPRPDDCTGSSAE"
     gene            4664..>5049
                     /locus_tag="VF633_12135"
     CDS             4664..>5049
                     /locus_tag="VF633_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8471853.1"
                     /translation="MSTLTTSISSAAAAGQKAVALDAAMVAKLTDMAGDFAISASVAI
                     LILVITVFAAKWAAAGTRKTLGKVKGFRHDPTVLSFAVQVVRVLVLLVGFIAVLQRLG
                     VQTTSIIAVLGAASLAVGLALQGTLS"
BASE COUNT          852 a         1523 c         1761 g          913 t
ORIGIN      
        1 cctcgttctg tagacggcgt cgctgtcggg ggacgtgtcc agtatacgac ccggttcaac
       61 ttgcctgact gatcagacga tccgctaagc ttgacgcccc ggagggaaac ctccggggcg
      121 ttttcgtgcg ggcttcatcc atgcgtcttt gtggggcctc aagcggtctg caggtccgct
      181 ttcgcgctct tcattgtggc ccttctcgac ccgttgcgga cattgcggga gtgcgctctg
      241 cggcgcacgg agtctctttg gatcaacgct gttggcgctc agcgggtaac acggccatca
      301 aggcccgagt gaacccgacc ggagcgacgg tgaggtggtc ttcgtcggtg agaaccaccg
      361 aatggacctg aaggtttgcg tagccacgcg acttcaaaat ccgctccatc gatcgcacgt
      421 ctcccaccat gtcgatgttg ggattgcgcc gactggggcc aggagtttcg tattcgccga
      481 tatacatgaa tacatcggct cggaggtcgc tgtgcgtgcg tgcgtacctc gcttccatcg
      541 tcatgatgtg gcgcgcgtcg aaccagaacg acgggctgcc cagcacatag ccctggaaca
      601 gcgtgggatc ggtgaagagt atctgggcgc cgagcagtcc gccgtacgaa tggcctagca
      661 gcacacggcg ggcgggatgg gcgcgaaacc gattttcgat gaaagggatc gcctgctgct
      721 tgaggtaggt ctggtaggcc gggccttcgc catggagcat ggaactggag cgcgctggtc
      781 cgtttgctgt cggcgtgtaa tcgcgattgc gactcaccgt ggcgctgtcg ccctgcgaat
      841 aggaaaggcc gaccaggatg aactcttcaa tcactggtcc ctcaaggttc acccggcgag
      901 cgatctggcg aatgatggga aaggcatagt cggcgtcggt tacataaagg actggatagc
      961 ggcgttcggg gctggtctcg tagctggcag gcaggctgac aaagacctca tagttacgcg
     1021 ctgacacagg gttgggcacc gtccagacct gggtgccttt cagcacataa ggcgcgcctt
     1081 gggcttgcgt ggcgttcgct tggggctggg cgtcggcgtt agcggtgcaa gccgaagcga
     1141 ggattgccgc caggaaggta accgccaggc cagggatttt catgtgcgac tcgataaagc
     1201 ctggccgcag caggccagaa cgcgaccgtg acgaagcccc cctcaatgtc aaacctcaac
     1261 tgtccgcttc ccacccgtct ccgccgctca gcgcgtccgc tcccagtccc gccaaagcaa
     1321 cgagctggga aaagcgacgg cttgcggtaa cgtcgttctg atcggggcgg cgggctccgt
     1381 tacggaggtc tttcatgcgg gttattggga tagcggcggc agccctggcc gtggcggcgt
     1441 gttcagctcc gttgttggcg gccacgattg gagaaggggc tcggcctgtg acggagcagg
     1501 ccgctgtcga tcccgccgcg gcggcctttg tcctgtcgct ctacggcgtg gagtccggag
     1561 ggaccggcga ggcggttccc gagaatgact gggatctgga ggcggtcagc tcggcgcgga
     1621 cagcggccct gttcgctgag gccaaggctc tgacgccggc aggctacatc ggctatcccg
     1681 agggtcatcc gctggtcgag tttgaggagt ggggctcctt gaagctggag gacgtggtca
     1741 cgacccagca cgggtccgac cgggccgacg tgtcgctgat cctggccttc acccagccga
     1801 acgtccgcct gaaccgaacc ttcaatctgg taaaagagga tggacggtgg cggctcgatg
     1861 acctgaaata cgaccagtcg gttcaaacgg cccagtccgg cgcccagtcc ggcccgaatg
     1921 cgccgatccc cggcctgctg gacgggtttc acagcttcat cgcggaggcg aaggcccatc
     1981 cgtttgagga ccaatgacag gctcgaggga ctgatccgaa acgaatgcag gccacagtcg
     2041 ttggcatgcg gtcgcgcccc tcggcgtccg tggaactctc gcccttgcgc gccttcgccg
     2101 aacttctgga tcgtctgtcc ctgacctcat cgcgcaatgc gaagttggtg ttgctgcgcg
     2161 actatctgaa ggcgacgccc gatccggaca ggggctgggc gctggcagcg ttgaccggcg
     2221 acctgagctt cgatgcggcc aagccggcga tgatccgacg ggcggtcgag gcgcgggtgg
     2281 attcggtgct gttcggctgg tcctacgatt acgtcggcga tctcgcggag accgtcgccc
     2341 tgatctggcc gggcaatccc gagcggcggc ccaaccggga gcctgagctg agcgaggtgg
     2401 tggatgcatt ggccatcgct aagcgcaccg aggttcaggg gctgatcgag ggctggctgg
     2461 atgccttggc gcccaagggg cggtgggcgc tgctcaagct ggtgaccggg gccttgcgcg
     2521 tcggcatgtc tgcacggctg gccaagacgg cggcggcgat gatccggccc gcctcgatca
     2581 tggagccgcc cgatcctgag ggtggcgaga cggcaaccgt gctcgacgcc gtggacgctt
     2641 cggacatcga ggaggtctgg cacgcggtcg cgccgcccta tgccgacctg ttcgcctggc
     2701 tggaggggcg ggcagagcga ccgagccccg atgcgccggg gcggttccga ccggtcatgc
     2761 tgtcggtcgc catcgatgag gccgtcgatt tccccaagct gagccccgcc gactatgccg
     2821 ccgaatggaa gtgggatggc atccgggtgc aggcagtgcg cgaggcgggc gtcgccaagc
     2881 tttattcccg cactggcgac gagatttcgg gcgcgtttcc cgacgtcatg gccgggctga
     2941 cgttcgaggg cgcgatcgac ggggagttgc tggtgtggag agacggcgtg gtcgcgccgt
     3001 tcggagacct gcagcagcgg ctgaaccgca agaccgtcga tgcgaagatg atggcgacct
     3061 acccggcggc ggtgatcgcc tatgacgtga tggccgagaa cggcgaggac ctacggacgc
     3121 tgtcgttgcg ggaacggcgg acgcggctgg agacgctggt cgcgggccat gagggtgacc
     3181 ggctgcacct gtcgcccttg gtggagtacg acagctggga aggcctcggg accctgcgcg
     3241 ccgatccgcc ggtgggggcg gcggccgagg ggctgatgct gaagcgctgg gattcggcct
     3301 atgtcgccgg gcgtcccaag gggccgtggt tcaagtggaa gcgcgatccg cacaccatcg
     3361 acgcggtgat gatgtacgcc cagcgcggcc acggcaaacg ctcgagcttc tattccgact
     3421 tcaccttcgg cgtctggacc gaggaagggc cgctgacgcc ggtcggcaag gcctatttcg
     3481 gattcaccga cgacgagctg aaactgctcg acaaattcgt ccgggattac acgatcgaac
     3541 ggtttggtcc ggtgcggtcg gtgcgggccg agcgcgattt cggcctggtg ctggagatcg
     3601 cgtttgaagg cctgaaccgc tcgccgcgcc acaagtctgg cgtggcgatg cggtttccga
     3661 ggatatcgcg catccgctgg gacaagccgg cgcgggaggc ggacacgctg gatagcgtca
     3721 tggacctgct cgacgcgatc gagagcggcg ggggcagggt ggcgaaggtg gtctaggggc
     3781 tcggcattcg ttgggtcccc ccggtctcgc tacgctcgcc ggaggatgac gacaggggga
     3841 gggacaccca aagcggaccc tggatccgac aggatcactc agtgatgaac gtctcaggtc
     3901 aatgggcggc ggaccgtccg aattcagggc aaatactacg ctacctatgc catagaaaac
     3961 cgatgttctt cagcccgaga atcccaactg gcgccacgat cgccctcgcg gtgtggtgcg
     4021 ccgcagcctc aggccgcgcc gagcctcagg atgagcccac gcagacaccc cgggaagcgc
     4081 tcgccctcgt ggcaggcggc gatctcgtgc tgcatgacgc cgcgatgatc aaggtcttca
     4141 ccaatgtcgc gccccgagcg gtcgtaagca gtccgctgca catcaccggg atcgtcgaag
     4201 gtggcctgtt ccaggaaggc gtctttccga tctacctgat gggccacgaa ggctgcgttc
     4261 tggcatcggc tcccgcccgt ccattgacgg actggatgac ggagggcgag gtggcgttcg
     4321 aagcgacctt ggtgttcagc gctccgcccg gatcatgggc gacgatcgtg tttgaacagg
     4381 atgtcgaata cgcggatgca ccggagcccc tggacgtgcg aacgcgggtg acgatcgcgg
     4441 gcgcggagga ccccgagtac ctcgttccgc gccccgacga ctgcacagga tcgtcggcgg
     4501 agtagagcat tcgccgtcca cgacgacttg atcagcacgc tctgcagact caaggtcggg
     4561 cgtgccttcc gcatccggca cgatgtctgc tttccaccgg ccgacccgta aaatgaagtc
     4621 ttccgggctt ccaaattgag ggtgaggcgt tagaccccgc ttcatgtcca cactgactac
     4681 atccatctcg agcgccgccg cagcaggcca gaaggccgtc gcgctggacg ccgccatggt
     4741 ggccaagctg accgatatgg cgggcgattt cgccatcagc gccagcgtcg ccatcctgat
     4801 cctcgtcatc accgtgttcg ccgcgaaatg ggcggcggcg gggacgcgca agacgctggg
     4861 caaggtcaag ggcttccgcc acgacccgac ggtgctcagt ttcgccgtgc aggtggtgcg
     4921 ggtgctggtg ctgctggtcg ggttcatcgc cgtgctgcag cggctgggcg tgcagacgac
     4981 ctcgatcatc gcggttctgg gcgccgcgtc gctggccgtg ggtctggcgc tgcaggggac
     5041 actgtcgaa
//
LOCUS       DASVTM010000446         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_735396,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000446 DASVTM010000000
VERSION     DASVTM010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735396"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VF633_12140"
     CDS             <1..1277
                     /locus_tag="VF633_12140"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX8471854.1"
                     /translation="RLILDLCGGEPSEVVLVGQVPADPVAFPFDPAYVKRLSGMDLED
                     DRIGTILGQLGFKVVAGGAGQPEPWTVTPPSWRRDVEGKADLVEEVARIVGFDHLPDT
                     PLPNQNPSSKGVLNPRQSRVRLARRALAAMGYAEAVTWSFTSQKTAALFGGGDERLVV
                     ENPIASELDCMRPSALPNLIQAAARNAARGHADAALFEIGPIYLGDGPKDQRTVIAGL
                     IAPHAARHWSGKDAADDALFELKGDLMALLEEIGAPVGSMQLSQGQNRDWWHPGRSAR
                     LQLGPKTVIAEFGALHPRVLKALDADGPMLAFEIVLDSVPEPKAKAKTRGSANLSVLQ
                     PLTRDFAFVVSDDKAAGDLVRAIAGADKALIEDVRVFDVYRGAGVPEGSKSVAIEIVI
                     QPREATLTEVEIEALSAKVVAAAAKVGGQLRS"
     gene            1411..2031
                     /locus_tag="VF633_12145"
     CDS             1411..2031
                     /locus_tag="VF633_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX8471855.1"
                     /translation="MHRRHLIQTGFAAAAASALGACATGPSQRQGTDPAYPIASDANA
                     PAYTSDELVAAGSRQLGIAAEAMGGAIERIFADQGDRPTGYIAGEEAAGAAAVGLRYG
                     RGALHMKDLGTAQDVFWQGISIGWDVGGNASRVFTLVYGLFTPDAIYRRYPGVEGSAY
                     LIGGVGVNYQRADGIVLAPVRTGVGLRLGANVGTMAYSRQRNILPF"
     gene            complement(2378..>2503)
                     /locus_tag="VF633_12150"
     CDS             complement(2378..>2503)
                     /locus_tag="VF633_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8471856.1"
                     /translation="DAMRRFYLDNKRISNARAKAELGWRPMYPTWREGLEAIRRA"
BASE COUNT          381 a          866 c          808 g          448 t
ORIGIN      
        1 cccgactgat cctagacctg tgcggcggcg aaccgtccga ggtcgtactg gtcgggcagg
       61 ttccggccga tcccgtggcc ttcccgttcg accccgccta cgtgaagcgc ctgtcgggca
      121 tggatctcga agacgaccgc atcggcacca tcctcggcca actcggcttc aaggtcgtgg
      181 ccggaggtgc aggccaaccc gagccatgga ccgtgacccc gccgtcgtgg cgtcgcgacg
      241 tcgagggcaa agccgatctg gtcgaggagg tcgcccgcat cgtcggcttc gaccacctgc
      301 ccgacacgcc cctgcccaac cagaacccgt cgtcgaaggg cgtcctgaac ccgcgtcagt
      361 cccgcgtccg cctcgcccgt cgggcattgg cggccatggg ttacgccgag gctgtgacct
      421 ggtccttcac cagccagaag accgcagccc tgttcggtgg cggcgatgag cgtctggtgg
      481 tcgagaaccc cattgcgtcc gagctggact gcatgcggcc ctcggccctg ccgaacctga
      541 tccaggccgc tgcgcgcaac gcagcgcgag gtcacgccga cgccgccctg ttcgagatcg
      601 gcccgatcta tctcggcgat ggtcccaagg atcagcgcac ggtgatcgcc ggcctcatcg
      661 ccccccatgc cgcgcggcac tggagcggca aagatgctgc cgacgacgcg ctgttcgagc
      721 tcaagggcga cctcatggct ctgctggagg agatcggtgc gccggtcggc tccatgcagc
      781 tcagccaagg ccagaaccgc gactggtggc atcccggtcg ttcggcccga ctgcagctcg
      841 ggcccaaaac cgtcatcgcc gagttcggcg ccctgcaccc gcgcgtgctc aaggcgctgg
      901 acgcagacgg cccgatgctg gcgttcgaga tcgttctgga cagcgtcccc gagcccaagg
      961 cgaaagccaa gacgcgcggc tcggccaacc tgtcggtgct ccagcctctg acccgcgact
     1021 tcgcgttcgt ggtctcggac gacaaggcgg cgggcgatct ggtccgcgcc atcgccggcg
     1081 cggacaaggc gctgattgag gacgtccggg tgttcgacgt ctatcggggc gccggcgtgc
     1141 ccgaaggctc gaagtcggtg gcgatcgaga tcgtcatcca gcctcgcgaa gcgacgctga
     1201 ccgaggtcga gatcgaagcc ctgtcggcca aggtcgtggc ggcagcggcg aaggtgggcg
     1261 gtcaactgcg gagctgaccg gtccgatccg ccccctgtcc tcctctgcgg caaggttctg
     1321 ttaactctga cgccgcaagg ctgccgcaaa agtccgcgag gttggcgata acgctcgcct
     1381 gacgggcgac cttctgccgg ggacttcgcc atgcaccgcc gccacctgat ccagaccggt
     1441 ttcgccgccg ccgccgcctc tgcgctgggg gcctgcgcca ccggcccctc gcagcgtcag
     1501 gggaccgatc cagcctatcc gatcgcctcc gacgccaacg ctcccgccta tacgtccgat
     1561 gaactggtcg ccgccggttc tcgccagctc ggcatcgccg ccgaggccat gggcggcgcg
     1621 atcgaacgca ttttcgccga tcagggcgac cgccccaccg gctacatcgc cggcgaggaa
     1681 gccgctggag ccgctgcagt gggtctacgc tacggacgcg gcgccctgca catgaaggac
     1741 ctcggcacgg ctcaggacgt cttctggcaa ggcatctcga tcggctggga cgtcggcggc
     1801 aacgccagcc gggtcttcac cctcgtctat ggcctgttca cgcccgacgc catttaccgc
     1861 cgctatcccg gtgtcgaggg ctcggcctat ctgatcggcg gcgtcggcgt gaactaccag
     1921 cgcgccgacg gtatcgtgct cgctccggtc cgtacgggcg tgggcctccg cctcggcgcc
     1981 aacgtcggca ccatggccta tagccgccag cgcaatatct taccgttcta agcgatatga
     2041 gctggctctt tgcgcttctc ttcgcagcgg ctgttttagt gtcagtgttg agcggccttt
     2101 tggcgttcta tctcgacctc cgtcggtcaa cgttggtcgg agcggggcaa cttccacgtt
     2161 cgacacgtga gattttccgc atcgagcctt cgacaatggg caaggtcgtg tttggctgga
     2221 tcggcgttga ctggtcgtat gtcttctcga aagcgctgga cgaaagtcca gatgctccta
     2281 cgcgaaagtg ggcgcccgtc cttcgaaagg gcctcctgac tagtgctgtt ctaggtggcc
     2341 tcacggcagc ttgtggaatt ctccggctca ttctcggcta ggcccggcga atcgcttcca
     2401 gcccctcgcg ccacgtcggg tacataggcc gccagccgag ttcggccttg gcgcgggcgt
     2461 tcgagatgcg tttgttgtcg aggtagaagc gtcgcatggc gtc
//
LOCUS       DASVTM010000447         6721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_98267, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000447 DASVTM010000000
VERSION     DASVTM010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98267"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="rpoN"
                     /locus_tag="VF633_12155"
     CDS             <1..847
                     /gene="rpoN"
                     /locus_tag="VF633_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX8471857.1"
                     /translation="KLQLIERNRFDPAMAAMLDNLELLAKRDLSALRQVCGVDAEDMS
                     EMIAELKGLTPRPGAGFGHETAQTVIPDVHVRPDPAGGWRIELNSDTLPRLLVDKRYH
                     ATIAAGARSETEKAFVADCAAQANWLVKSLDQRAKTILKVGSEIVRQQDAFLAFGVEF
                     LRPLNLKTVADAIGMHESTVSRVTSNKYVSTPRGVFELKFFFTASINATGDGAAHSAE
                     AVRHRIKSMIDHEGRDGDVLSDDRIVELLNEAGIDIARRTVAKYREALRIPSSVQRRR
                     MLKFA"
     gene            921..1598
                     /gene="raiA"
                     /locus_tag="VF633_12160"
     CDS             921..1598
                     /gene="raiA"
                     /locus_tag="VF633_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX8471858.1"
                     /translation="MQVQVSGKQVDVGEALGSRISQELEDGVGKYFERGGQDAEVVVS
                     KDGYGFKVDCWVRLASGESLVTSGTGGDAHSAFTDSLDKLEKRVRRYKRRLKDHHIGP
                     KGLSPEKTEMAAASVARSIVLRNPDDVEDDVFGDTGTSGDPPPVGMVIAETQSEIRTI
                     TVGRAVLELDMTGYPIVLFKNAAHGGLSVVYRRPDGNVGWIDPERTSGLSGAVPPVVN
                     GHATAGS"
     gene            1692..2156
                     /locus_tag="VF633_12165"
     CDS             1692..2156
                     /locus_tag="VF633_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX8471859.1"
                     /translation="MDIGDLLVEDGIVLRSGASSKRQALHVVAEAAANAMGLDSGRVM
                     DALLEREALGSTGLGSGVAVPHARIEGLQRIFGVFVRLDTPVPYGSVDDRPVDLLFAL
                     FAPPRDGAEHLRALAAVSRAVRSPELRENLRQARTVDAARALFVTDRAATAA"
     gene            complement(2392..2943)
                     /locus_tag="VF633_12170"
     CDS             complement(2392..2943)
                     /locus_tag="VF633_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX8471860.1"
                     /translation="MTAGWTEDRVGALKKLWLEGQSASQIAKQLGGGVTRNAVIGKVH
                     RLGLSGRATPSQPARTTTTTTFRAAKTRTTPPKQDFGQPSAPRRVEGERVLAIAPRAT
                     SPVPPTPLPVVVEQPGTATVLTLGAHMCKWPIGDPSSREFSFCGRRSSEGVYCIEHAR
                     VAYQPQVKKNGTTELTRSLRRYI"
     gene            3283..4104
                     /locus_tag="VF633_12175"
     CDS             3283..4104
                     /locus_tag="VF633_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8471861.1"
                     /translation="MTDLASTRPSGLPTPRRYDGVNWVGVQTLYLREVRRFWKVGAQT
                     VAAPVVTTLLYMLVFVVALKDSRPPIDGTPFAEFVAPGLIMMAILNNAFANASSSLIQ
                     AKIMGTSTDFLTPPLSPLELTIGFSLGAATRGICVGLVTAICVLPFARLGVTNILVIL
                     YFGLIASLLMGMLGVLAGLWSEKFDQLAAVQNFVIMPMTFLSGTFYLVQNLPGPFAAI
                     SHFNPFFYLIDGFRYGFIGHAEGSLWIGAVSSLALTLALAVCCWLVFRSGWRLKS"
     gene            complement(4116..4829)
                     /gene="pdeM"
                     /locus_tag="VF633_12180"
     CDS             complement(4116..4829)
                     /gene="pdeM"
                     /locus_tag="VF633_12180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEX8471862.1"
                     /translation="MTLTPKRSTCGGLAVRIAGERCVLRCSGAMHVVEHDVLIVADLH
                     LEKGSAFAMRGQMLPPYDSAATLARLEEEVATVNPARVVLLGDSFHDSRSIPRMAETD
                     RETLERMAFGRDWLWLEGNHDREALGTDLARLPGSVIDTLSLGALRLVHEPQPGDQPG
                     EVAGHLHPSARVAAYGRGVRKPCFVTDGSRIILPAFGAFTGGLNVRDPVIASLFASPP
                     LAAVPGRDQVHAIAWSSLV"
     gene            complement(4862..>6721)
                     /locus_tag="VF633_12185"
     CDS             complement(4862..>6721)
                     /locus_tag="VF633_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX8471863.1"
                     /translation="PGAPPVVDVLVSEGHVPWAGHTGQHAIPEVYDAIKQAGMTLIFV
                     NTRWQSEFVFQKLWEINEDNLPIGLHHGSLAAEQRRKVEAAMSRGDLRAVVCTSTLDL
                     GIDWGDVDLVIQLAAPKGASRLVQRIGRANHRLDEPSRALMVPASRFEMLECQAAREA
                     VAENAFDWEPHHIGTLDTLAQHIMGVACSEPFQLDALHQEIASCGPYADLPYEQFEEV
                     VDFVATGGYALRTYDRFARIVKDRDGFWRVRNAITAQRHRMNVGAIVAAATLNVRVAS
                     RRSGAAKQLIGGRKVGEAEESYFEQMSPGDTFVFAGQIWAFQGINGMDALVSHSQDSD
                     PKIPSWGGSKFPMSTSLADRVRALIQNRDHWRVLPNDVQEWLELQEAKSAIPQADSML
                     IETFQRGSRHFMVAYPFEGGLAHNTLAMLLTRRLDRAGVGPLGFVCTDYSLAIWSIKP
                     MTSKADGGTLDYDDLFQPDMLGDDLESWLEESFMMKRTFRNCALISGLIERRQPGQEK
                     TGRQVTFSTDLIYDVLRRHQPDHLLLRTARADAAAGMLDVARLGQLLSRIAGHILTKA
                     LERPSPFSVPVLVVIGRERVGGGDAADMILSETAEALIAEVMDDAPPELQGAA"
BASE COUNT         1143 a         2163 c         2200 g         1215 t
ORIGIN      
        1 gaagctgcag ctgatcgagc ggaaccgctt cgatccggcc atggcggcga tgctggacaa
       61 tctggagctt ctggccaagc gcgatctttc agccctgcgc caggtctgcg gcgtcgacgc
      121 cgaagacatg tccgagatga tcgccgagct gaagggcctg accccgcgtc ccggcgcagg
      181 cttcggtcac gagacggccc agaccgtcat ccccgacgtt cacgtacggc ccgacccggc
      241 cggcggctgg cgcatcgagc tgaactccga caccctgccg cgactgctgg tcgacaagcg
      301 ctaccatgcg acgatcgcgg ccggcgcgcg gtccgagacc gagaaggctt tcgtcgccga
      361 ttgcgctgcg caggctaact ggctggtcaa gtcgctggac cagcgcgcca agaccatcct
      421 caaggtcggc tccgagatcg tgcgccagca agacgcgttc ctggcattcg gtgtcgagtt
      481 cctgcggccg ctcaatttga agaccgtcgc cgacgcgatc ggcatgcatg aatcgacggt
      541 cagccgggtc acctcgaaca aatacgtctc gaccccacgt ggtgttttcg agctgaaatt
      601 cttcttcacc gcctcgatca acgccaccgg tgatggcgcg gcgcattcgg ccgaggctgt
      661 ccgacatcgg atcaagtcga tgatcgatca cgaaggccgt gatggcgacg ttttgtccga
      721 cgaccgcatc gtcgaacttc tgaatgaggc aggcatcgat atcgcgcgcc gtacggtcgc
      781 caagtatcgg gaagccttga ggattccgtc ctcggtccag cgtcgccgga tgctgaaatt
      841 cgcctgagac atcacgcaag cgtaaccaca aaacggtgat cgcgattgac accaaatcga
      901 accggtcgcg ttctatgtgg atgcaagtcc aagtgagcgg caagcaagtg gatgttggcg
      961 aagcgttagg ctcgcgcatc tcccaggaac tcgaagatgg agtcggaaaa tatttcgaac
     1021 gcggcggcca ggatgccgaa gttgtagtct ccaaggatgg ctacgggttc aaggtggact
     1081 gttgggtccg cctggcgtcc ggcgagtctc tcgtcaccag cggcacgggc ggtgacgccc
     1141 attcggcctt caccgacagt ctcgacaagc tcgagaagcg ggtccgtcgc tacaagcgcc
     1201 ggctgaagga tcaccacatc ggtcccaagg gcctgtcgcc cgagaagacc gagatggcgg
     1261 cggcctccgt ggcccgcagc atcgtccttc gcaaccctga tgatgtcgag gacgatgtct
     1321 tcggcgatac ggggacctcg ggtgatccac cgccggtggg tatggtcatc gccgagaccc
     1381 agtccgagat ccgcaccatc acggtcggac gcgcggttct ggagctggac atgaccggct
     1441 atccgatcgt gttgttcaag aacgcagcgc atggcggcct ttcggtcgtc tatcgccgcc
     1501 cggacggcaa cgtcggctgg atcgacccgg agcgaacgtc gggtcttagc ggcgcagttc
     1561 cgcccgtcgt caacggacac gcgacagcgg gttcctgaca ccagttcaag ttgaaggctt
     1621 tctttggtgg ggcggagcgg ctaaagcccg cccgccccat tcgggtcaga tgtatcgagt
     1681 cggggatggt catggatatc ggtgatttgc tggtggagga cggtatcgtt ctgcgcagcg
     1741 gcgcgtcgtc caagcggcag gcccttcacg tggtcgccga agccgcagcc aacgccatgg
     1801 gtctggactc cgggcgcgtg atggacgccc tgttggaacg cgaggccttg ggttcgaccg
     1861 gactgggttc cggcgtcgcc gtgcctcatg cccggatcga aggcctgcaa agaatttttg
     1921 gcgtattcgt gcgactggat acgcccgtac cttacggctc ggtcgacgat cgccccgtcg
     1981 atctgctgtt cgccctgttc gctccgccgc gtgatggcgc cgagcatctg agagcgttgg
     2041 ccgccgtgtc ccgagctgtg cgctcgcccg agttgcgcga aaacctgcgt caggcccgca
     2101 cagtcgacgc cgcccgcgca ctgttcgtca ccgaccgcgc cgccactgcg gcctgaggcg
     2161 acccatgcgg taagattttg gtggtccggc gtccctctga agagagggtg ccggggctgc
     2221 gtcaacccgg ataaccgcag ccccgcccca tcacccgccg actggggagt caggcggggt
     2281 ggcagtatgt ccgccgaggc ggatcaactc gtaagcacac ggacgcggat gccgcgatca
     2341 cccgctgcgc tcaagccgcg agcgaccgcg tcgcgagcga tagcgctctc cctagatata
     2401 ccgacgcagc gagcgggtga gctcggtcgt gccgttcttc ttgacctggg gctggtaggc
     2461 gacccgggcg tgttcgatgc agtagacgcc ttccgaggaa cgacggccgc agaagctgaa
     2521 ctcgcgcgac gagggatcgc cgatcggcca tttgcacatg tgagcgccga gcgtcagcac
     2581 ggtcgctgtg ccgggctgtt cgacgacgac cggcagaggg gtcggcggta ctggcgaagt
     2641 cgcgcgtggc gcgatggcca ggacccgctc accttcgaca cgacgcggcg ccgacggttg
     2701 accgaagtcc tgcttgggcg gggtcgtccg ggtcttggcg gcgcggaagg tcgtggtcgt
     2761 ggtggtccgt gcgggttgcg atggcgtcgc gcggcccgac aggccgagac ggtgcacctt
     2821 gccgatcacg gcgttgcgag tgacgccgcc gccgagttgt ttggcgatct ggctggccga
     2881 ctggccttcc agccagagct tcttcagcgc gcctacgcgg tcttcagtcc agcctgcagt
     2941 catgtcgccc tccgggggga tcaagattca acatatggtg accagaccag cctcccggac
     3001 ccgtcaccta ccactaatcg taaacgagac cttaacagac gcaagatgtg cgaatcagtc
     3061 gtccacagga atccagatgt tgaatcggcg ttatgtgggg gaggaacagg gagaaggcag
     3121 taggcagtag gcagtaggca ggagaagagc ggcaggaccc gagcgacgcg cggcggccaa
     3181 gcgccaagcc ccaaagccct accggcactg cctactgcct actgcctatc tgcctcctcc
     3241 ctcccccttg catccgtccc cctgacgccg catctgcagc ggatgaccga tctcgcttct
     3301 actcgccctt ccggactgcc tacgccgcgt cgatatgacg gcgtcaactg ggtgggggtg
     3361 cagacgctgt atctgcgtga ggtccggcgg ttctggaagg tcggggctca gacggtggcg
     3421 gcgcctgtcg ttacgaccct gctctatatg ctggtctttg tggtggcctt gaaagactcc
     3481 cggccaccca tcgacgggac gcctttcgct gagttcgtag cgcccggcct gatcatgatg
     3541 gcgatcctga acaacgcttt cgccaacgcc tcgtcgagcc tgatccaggc caagatcatg
     3601 ggcacctcga ccgacttcct gacgccgccg ctgagcccgc tggaactgac catcggcttc
     3661 tctctgggcg ccgccacgcg cggcatttgc gtcgggctgg tgaccgctat ttgcgttctg
     3721 ccgttcgcca ggctgggcgt gaccaacatt ctggtcatcc tctatttcgg cttgatcgcc
     3781 tccctgctga tggggatgct gggggttctg gcgggtctgt ggtccgagaa gttcgaccaa
     3841 ctcgcagcgg tccagaattt cgtcatcatg ccgatgacct tcctgagcgg caccttctat
     3901 ctggtgcaga acctgcccgg accgttcgcg gcgatcagcc acttcaaccc gttcttctat
     3961 ctgatcgacg gcttccgtta tggcttcatc ggccacgccg agggcagcct ttggatcggg
     4021 gcggtgtcga gtctggcgct gaccctggcg ctcgccgtct gctgctggct ggtgttccgc
     4081 tcgggctggc ggctgaagag ctagaccagc cccgactaga ccagactgct ccacgcgatg
     4141 gcgtgaacct gatcgcgccc cggcacggcc gcgagcgggg gtgaggcgaa cagactggcg
     4201 atgactggat cgcgcacatt cagaccgccg gtaaacgcgc cgaacgccgg caggatgatg
     4261 cggcttccgt ccgtgacgaa gcacggcttg cgtacgcctc gaccataggc cgcaactcgc
     4321 gctgagggat gcaggtgccc tgcgacctcg ccgggctggt cgccgggctg aggctcatgc
     4381 accagccgca gcgcgccgag cgacagggtg tctatgaccg agccgggcag gcgggcgagg
     4441 tccgtgccca aggcctcgcg gtcgtggttg ccttccagcc aaagccagtc gcgtccgaaa
     4501 gccatccgct ccagcgtctc gcgatcggtc tcggccatgc gcgggatcga gcggctgtcg
     4561 tggaaactgt cgccgagcag gacgacccgc gcagggttca ccgtcgcaac ttcttcctcg
     4621 agccgggcga gggtggcggc gctgtcatat ggcggcagca tctgaccgcg catggcgaag
     4681 gccgagccct tttccagatg caggtcggcg acgatcagga catcgtgctc gaccacatgc
     4741 atcgcgccgg aacagcgcag cacgcagcgt tcgccggcga tcctcactgc cagaccgccg
     4801 cacgtgcttc gtttgggtgt gagggtcatg cgccattcct cgacaagcat cgtccgcgcc
     4861 gtcatgccgc tccttgcagt tctggcggcg catcgtccat gacctcggcg atcagggcct
     4921 cggcggtctc gctcaggatc atgtcggccg cgtctccgcc accgacccgt tcgcgcccga
     4981 tcaccaccag aaccggcacc gagaacggtg aaggccgctc cagcgccttg gtcagtatat
     5041 ggcccgcgat gcggctcaga agctgaccca gccgggccac gtcgagcatg cccgccgccg
     5101 catcggcgcg cgcggtccgc agcagcaaat ggtcgggttg gtggcggcgc agcacgtcgt
     5161 agatcaggtc cgtggaaaag gtcacctgcc gcccggtctt ttcctgaccc ggctggcgtc
     5221 gctcgatcaa gcccgagatc agggcgcaat tgcggaaggt gcgcttcatc atgaagctct
     5281 cctccagcca cgattccaga tcgtcgccca gcatgtcggg ctggaacagg tcgtcgtagt
     5341 cgagcgtgcc gccgtcggct ttcgaggtca tcggtttgat cgaccagatg gccagcgaat
     5401 agtcggtgca gacgaagccc agcggcccga cgcccgcccg atccagccgt cgcgtcagca
     5461 gcatggcgag ggtgttgtgg gcgagtccgc cctcgaacgg ataagcgacc atgaaatgcc
     5521 gactgccgcg ctggaatgtc tcgatcagca tcgagtccgc ctgcgggatg gccgatttcg
     5581 cctcctgaag ctccagccat tcctgcacgt cgttgggcag gacgcgccag tggtcgcggt
     5641 tctggataag ggcccgaaca cggtcggcca aagacgtgga catgggaaac ttcgacccgc
     5701 cccaggacgg gattttcgga tcggagtcct gcgagtggct aacgagggcg tccatgccgt
     5761 tgatgccctg gaaagcccat atctgaccag cgaagacgaa ggtgtccccc ggcgacatct
     5821 gttcgaaata gctctcttcg gcctcgccga ccttgcgccc gccgatcagc tgtttcgccg
     5881 ccccgcttcg ccgtgaagcc actcgcacgt tcagggtcgc ggctgcgacg atggccccga
     5941 cgttcatgcg gtggcgctgg gcggtgatgg cgtttctcac ccgccagaag ccgtcgcgat
     6001 ccttcacgat cctcgcaaac cgatcgtagg tgcgcagggc gtatccgccg gtggcgacga
     6061 aatcgacgac ctcctcgaac tgctcgtagg gcaggtcggc ataggggccg cagctggcga
     6121 tttcctgatg cagggcgtcg agctggaagg gttcggaaca cgccacgccc atgatgtgtt
     6181 gggcgagcgt gtcgagggtg ccgatgtgat ggggctccca gtcgaaagcg ttttcggcca
     6241 cagcttcgcg ggcggcctga cactccagca tctcgaaacg gctggcgggg accatcaggg
     6301 cgcgcgacgg ctcgtccaga cggtgattgg cgcggccgat gcgctggacc agacggcttg
     6361 cccccttggg cgcggcaagc tggatcacca gatcgacgtc gccccagtcg atgcccagat
     6421 ccagcgtcga ggtgcagacc acggcgcgca ggtcgccgcg cgacatcgcc gcctcgacct
     6481 tgcggcgctg ctcggccgcc agcgagccgt ggtgcaggcc gatggggaga ttatcttcgt
     6541 tgatctccca cagcttctgg aacacgaact ccgactgcca acgggtgttc acgaagatca
     6601 gggtcatgcc cgcttgcttg atcgcgtcat agacctcggg gatggcgtgc tggccggtat
     6661 ggcccgccca tggaacgtgg ccttcagaca cgaggacgtc gacgaccggg ggcgcgccgg
     6721 g
//
LOCUS       DASVTM010000448        36209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_147520,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000448 DASVTM010000000
VERSION     DASVTM010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 36209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36209
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147520"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF633_12190"
     CDS             complement(<1..329)
                     /locus_tag="VF633_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471864.1"
                     /translation="MARPDPTLATQPKTPALAVLFLVVFINLVGFGLVVPLLPFFAQS
                     LKAEAWQITLMFSAYSLGQFFAEPFWGRLSDRIGRKPVLLITLAANAIGYLALAFVPN
                     IWLAIAVR"
     gene            complement(391..2370)
                     /locus_tag="VF633_12195"
     CDS             complement(391..2370)
                     /locus_tag="VF633_12195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8471865.1"
                     /translation="MYFQNVAATALALFVCVAGSLAHAHEPLPGVGDPPPLAAYGALP
                     SLELVQLSPSGQRLALITVIGETRALALVDLATNHSMGGVGVGASKVRDLQWISNDRV
                     LVTTSSTESLPELGIIRRELFIGQVYDPARRRVTGLIRGDSGLFPALMSGVDVAPPGP
                     RPDVLVRAFSFTEPGKINLYRIDAETGRMREAESMDRDVDDYVLDPNGRSLARSDFDD
                     RTKIWKLLLRDGSDFQENWRVTAPLDAPAIIGLGMRGESVIVAADRPDLKRNDREEAD
                     YYDVNLATGAWRAVRFEFDPTDLLFHPVTRRLMGATRLDDEGRRYSFADEDAGNLWAS
                     VAQTFPDGAPSLVSWSNDLKTAIVFTNGANDSGTYHLIDLTDSSTLRVGAAYSAVPAD
                     QVAPVTLVAYKAADGLEIRGYLTVPVGREAKNLPLIVLAHGGPASRDVREFDWWAQAL
                     ASRGYAVLQANFRGSTGRGEDFLEAGYGEWGRKMQTDLSDGVRHLVEQGVADPGRVCI
                     VGASYGGYAALAGPTLDHGVYRCAVSVAGVSDLRGMVNYAASRGERRDNGAVRYWNRF
                     MGGDGPGDRSLDARSPARQADKADAPILLIHGRDDTVVPIDQSRSMAGALRRAGKPVE
                     LIELSGEDHWLSRGETRIRMLTETVRFLETNNPPH"
     gene            complement(2560..3081)
                     /gene="infC"
                     /locus_tag="VF633_12200"
     CDS             complement(2560..3081)
                     /gene="infC"
                     /locus_tag="VF633_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX8471866.1"
                     /translation="MQTPPVKDGPPMNHDIRAPRVLLIDQNGEKQGVMPTSAALEAAE
                     EAGMDLVQIVSTSEPPVAKILDYGKFRFQEQKKKAEARKRQKVVELKEIKLRPNIDIH
                     DYEVKAKAMHRFFDEGDKVKVTLRFRGREMAHPELGMKLLNKVQADFDEIAKVEYAPR
                     MEGRQMIMILAPK"
     gene            complement(3220..4206)
                     /locus_tag="VF633_12205"
     CDS             complement(3220..4206)
                     /locus_tag="VF633_12205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX8471867.1"
                     /translation="MPPVTSVPKVLFVSSNRIGDCVISSGVIREIERQLPGAAITVAC
                     GRPPAPFFRSAPGVERAIVLDKKKLAGHWIDLWKQVATTRWDMVIDIRGSALSYLIPA
                     KRRVIYNRSWETGLRKVEMVSRLMGSETPLAPEIFLDDRARSEAAGVIDPQLAGAAGP
                     GPIIALAPIAHQPGKSWPADRWGALVEKLKADPRFEGWRFMAVGGPGDRPPATPALEA
                     AGERGIDFVGKGDILASAAAIERATLFVGNDSGLMHVSAALGRPTLGLFGPTEWWLYG
                     PWGPRTRIAASNETRGEFSPIEDLTVDHVFDAVVALHTDFVANSAGDGSVKP"
     gene            4276..5049
                     /locus_tag="VF633_12210"
     CDS             4276..5049
                     /locus_tag="VF633_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8471868.1"
                     /translation="MTPTDQPETNDNDIQHLARPDGERLAYKQVPGEGPTIVWIGGFR
                     SDMEGTKALALDAAARERGWAFLRYDHFAHGQSSGDWRRATIGRWREDAVAVLDSLNG
                     PAILVGSSMGGWVALLAALARPEAVAGLVLINPAQDFTETLMWPGLADHERQAILRDG
                     ETVVVEEGLGSYVVTRAMFEEARNWLLLGGPIPIKAMTHILQGRADDTVPWRYSITLV
                     EQLTGGDVMLDLIEGGDHRLSQPADLTRMINAIDAMRKA"
     gene            5087..5827
                     /locus_tag="VF633_12215"
     CDS             5087..5827
                     /locus_tag="VF633_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HEX8471869.1"
                     /translation="MDTLSERWAKLEPTTNVVGPDDDLKRPAVLLFHGCGGMRPHLPL
                     YAAAAKAAGWRAFIVDSYAPRGWGRVFALSMVCTGAILRGNERGGDVLAAIDGVSRRS
                     DVDSGKIAVAGWSHGGWSIMEMLAAPRTHSGELGIADPSIVCLSGLKGAWMGYPYVGF
                     LAVGRNRPWRHHPKTEVMIAKHDHLTTVRNAEQVFEGHRGRGTVIETWVADGTHSFDE
                     PTSAPPSRHDPALTEVAVKRFGQFLIGL"
     gene            complement(5824..6288)
                     /locus_tag="VF633_12220"
     CDS             complement(5824..6288)
                     /locus_tag="VF633_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8471870.1"
                     /translation="MSAHRTTVEWKRDGQAFIDKAYSRAHVWTFDGGAVVQGSSAPES
                     VPVPMSDVTAVDPEEAMVASLSSCHMLWFLAFAAGDGLVVDRYLDEASGTLGRDDKGR
                     RYMDKVTLRPVTTFFGVAPTQGELDALHHSAHEHCEMAHSVRAEILVEASIG"
     gene            complement(6293..7336)
                     /gene="trpS"
                     /locus_tag="VF633_12225"
     CDS             complement(6293..7336)
                     /gene="trpS"
                     /locus_tag="VF633_12225"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX8471871.1"
                     /translation="MSDATPTLPAYTGPRRVLSGIQASGALHLGNYLGALKRFTELQD
                     GGAPCFLFVADLHAITVWQDPALLTAQTREIAAAYLASGLDPAKSVIFPQSAVRAHAE
                     LAWIFNCVARLGWLDRMTQFKEKSGKHKERSSVGLYTYPVLQAADILLYKATEVPTGE
                     DQKQHLELTRDIAQKFNNDFGVPGFFPLPEPLTQGPAPRVMSLRDGAAKMSKSDPSDQ
                     SRINLTDDADTIAGKIRKSKTDMGVMPEVGDVLDDRPEVKNLIAIYAALSDQTKDAVT
                     AEFAGQGFGAFKPRLADLAVEAMAPITAEMRRLMADTAEIDRVLKDGAERAAAIADPV
                     VDEVKKIVGFWRA"
     gene            complement(7376..8974)
                     /gene="murJ"
                     /locus_tag="VF633_12230"
     CDS             complement(7376..8974)
                     /gene="murJ"
                     /locus_tag="VF633_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX8471872.1"
                     /translation="MSLARNTIVQSGLTLGSRLLGFGRDLLLSARFGQGPMMDAFTTA
                     LMLPNMFRRLFAEGAFAQAFVPIYGGVRARDGEEAAAVTASEALSFMFAVVAGFCIVL
                     QLAMPWIMPVLLSAYRNDSGVMFAAVTAAQLTMPYLACMTIASLLSGVLNTSGRFALS
                     AGVPVFLNLCTLVPLLAPEFMTISQPHILDAVSIAVTISGVLQAGLLWWGVRRLGVML
                     KLSWPRLTAGVKKTLALAIPGVLAGGALQINSVVSQFLTGSNPGARSVLYNADRLYQL
                     PLGLVGVAIGLALVPRLTKAFVSGDHEGGRRTLDDGLTLSMAFTLPAAVALFVIPFFI
                     IDATVTRGAFTSLDAARTADVLRQFAWGVPAFVLAKVLTPPFFAREDTRRPMIFAVAS
                     VLVTVVLGASLFYWFSHIGIDGVLGLAIATSTSAWINVALLIGTLIRENTWRPSANFV
                     SRIMRVLAASGVMAALLLAAGIFYPELSKLFLAKEIAVIVVCGVGAVIYCFCLFLFGA
                     VTPSELKAVLRREPGAAASGGGLE"
     gene            complement(9018..11726)
                     /gene="glnD"
                     /locus_tag="VF633_12235"
     CDS             complement(9018..11726)
                     /gene="glnD"
                     /locus_tag="VF633_12235"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX8471873.1"
                     /translation="MTPVSLTSSRTGLDDRLDALVGGSDVRAGVAALLRSHYAAARDK
                     IALRLAAGLGGVEVARQYSAAADDILIALWRFTTETLYPSHNPTEAEKLTLIAVGGYG
                     RGVLAPFSDLDLLFLRPWKTTARTETVAEFVLYVLWDLGLKVGSSARSVDECLALSRS
                     DMTVRTALLEGRWLAGDQPLAEDFLWRFRGTVTNADPRPFIAAKLEEREARHTKAGAV
                     RYRVEPNIKDGKGGLRDLNTLFWIARALAPESPLGAAVLDDLLTSRERRTFEEAFDFL
                     WRVRTHLHLTAGRAEEKLTFDLQPEVARRMGWRGRGDEPAVERFMRRYFLVARDVGAL
                     TRAMSATLEARQQKKTMSLSRLIPGRKRNLGVAGFIEDGGRLSVESAQVFADDPVKLL
                     SLFVIADQHDLDVHPHAFSAVTRSLGLVTPKLRRDPRATAALLHILAHGQRPYRVLTI
                     MNETGLLGRFLPEWGRIVGQTQFNMYHAYTVDEHSLQAIGIINDIFRGKLKEDHPLSS
                     STIHLIADLEVLMLAMLLHDVGKGGERGQLEDGAIAARRACERLGVEPRRIELVVWLV
                     RNHLALSDYAQKRDTSDPQTIRDFAEIVGDPERLRLLLVLTVADIRAVGPGVWNNWKG
                     QLITALYTRVEALFRGEDVDLIDPLAEHAHLIARACETGAAAECRQGPGRIEATTEIA
                     LAARDRPGLFADLTAVLAAAGADVTGARVATAADGTVLDVFQVQDGADAPYGQAEPRR
                     LKTLVAALEAAARGEAPIDAPAMPIATPRRAVFDVRPVVMIDAGASESATVIEVSGAD
                     RPGLLAELSRTLSDLDLSIRSAHVAGFGERAVDSFYVVDRKGRKVFSELLLNEIQHGL
                     EAVLERTPAAPKGRAIVAARASARDVSDLNRPLRKAKAVSPEPEAR"
     gene            11887..12663
                     /locus_tag="VF633_12240"
     CDS             11887..12663
                     /locus_tag="VF633_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8471874.1"
                     /translation="MIPRLVLVAALAVMALSLGLFSHAWAGEGPEDGLGASDGSRSGS
                     ALGADRSELRREHWSDGLTMAAAGYGPVGGDAQLSREVLGEMNRMRSDPQAYVAVLQD
                     YRRQFQGAIVVRPGRINIQTREGVAAVDEAIAFLRRQKPVSRLSPDLILALTAGDHVA
                     DQGPTGFIGHYGSDGWDFVTRVSRRGGDPYGGENISYGYDTAREVMVQLLVDDNIADR
                     GHRTNLFRDGYVRAGVSCGPHALFNHMCVIDYGYEPVKGR"
     gene            complement(12726..15431)
                     /gene="mutS"
                     /locus_tag="VF633_12245"
     CDS             complement(12726..15431)
                     /gene="mutS"
                     /locus_tag="VF633_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX8471875.1"
                     /translation="MNAPLAHPPQSPSTPVSTDGVTPVMAQFLAAKAAQPDAIVFFRM
                     GDFYELFFKDAEIASGLLGITLTKRGKHQGEDIPMAGVPVHALDGYLARLIRAGQRVA
                     ICDQIETPAEARKRGSKSIVARDIVRVVTPGTLTEDSLLEARGANRLAAVAIRKGRAA
                     VAVVELSAGAVDSIACDLGDLAATLAAFRPSEVLVPDKLFSDDAVRPALDGSGGVVQA
                     MASAVAEPAAARDRVERLYGVASLDGFGAFEEAEVSALGLIAAYLETTQAGKVPALTP
                     PRRSGESGFLAIDPATRQSLEIDRTQRGEREGSLLACLDRTVTSGGARALAERISRPL
                     RDPVAINDGLDAVEWLLERRDLRRDLRDGLKASADVARAVSRLALGRGGPRDLAAIRS
                     GLTIAEGIAGLFVVSGNPLSAPPARIIGCLDRLTLSPDTAQLMVDLAEGLMEEPPHLA
                     RDGGFVRPGFRPELDAARTLRDDSRRVVADLEARAVADSGVPFKIKHNAVLGYFLETT
                     AKHAEPLFRVGPESPYIHRQTLAAQVRFTTVELSELDAKISQAGYRALAMEGETFEGW
                     RQTVQRLARPLQAVADALAELDATSALAEWAEEVGAVRPTVDSSLTFEVQAGRHPVVE
                     AAVKKAGDPFTPNDCRLDGAPIKGGGCPRLSIVTGPNMAGKSTFLRQNALLVILAQAG
                     SFVPARSMRLGVVDRLFSRVGAGDDLARGRSTFMMEMVETAAILTQATPASFVVLDEI
                     GRGTSTYDGLAIAWACAEALHDTNRARTLFATHYHELARLEERLDHVSNLSMKAKEWN
                     GELVFLHEAAPGAADRSYGVQVAKLAGVPPSVVARARSVLDRLESEKAANHRLDDLPL
                     FALAEPPPPPMKSAMDEAVAALDPDAMSPREALEALYRLKALKRK"
     gene            complement(15506..16414)
                     /locus_tag="VF633_12250"
     CDS             complement(15506..16414)
                     /locus_tag="VF633_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8471876.1"
                     /translation="MKPLPAQTPAGKLAMAVVTEPERAVGAVTTAPVGAREDAMRFLL
                     QTAADAGIKVVATRPDRDGERLLGQAWPFPSPFRVTVRTVPLADGLDRVEARARRSLE
                     RLGLPRGDVLLVSNAADLAGAEGRALWDRMKSLKDRGLYRRIGFCATMEDGPALLARR
                     YQPDVVQMPCNLLDQRAVSEGVLSDLAAMNIDVHLSSVFARGLLFASRDSLPPHLAEH
                     GVALSRVRRRLAEARVDPMQAALAFALSLPPATCVVASVASAAELRAILAASCAPKPD
                     LDWDALALTEPAALTAGPRIPISHAA"
     gene            complement(16512..17141)
                     /gene="maiA"
                     /locus_tag="VF633_12255"
     CDS             complement(16512..17141)
                     /gene="maiA"
                     /locus_tag="VF633_12255"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX8471877.1"
                     /translation="MILHSYWRSGTSYRTRIALNLKGLAYEQQTVDLRQRAHKTPEYL
                     ALNPQGMVPALEIDGAILTQSVAILEWLEDAYPEPALLPAGPLERARVRAMAALIGSD
                     IHPLNNLRVLGAVRGLGADQNGVDAWAGGWIKAGFDALEQMVAQYGKGWSFGATPTLA
                     DCYLIPQLYSARRFNLDLTPWPKLLAVEQAAETHSAFVAARPDIQPDAD"
     gene            complement(17138..17830)
                     /locus_tag="VF633_12260"
     CDS             complement(17138..17830)
                     /locus_tag="VF633_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8471878.1"
                     /translation="MTDWLFPPAPTPSLPIVGEPAQAGRRFPVRRILCVGQNYAAHVR
                     EMGGDPSRPAPFFFSKPADAVVTDGKDPAYPSATTNLHHEVELVVAIGSTSEGSIGAI
                     GWAVGVDLTRRDLQAAAKEKGRPWDASKGFDQSAPCGPITLGDLPEPCASITLAVNGV
                     AKQAGDLSDMIWNPHEIVAKAGELWTLQPGDLIFTGTPEGVGPLVPGDRVEASIDGLT
                     TLVFTVAETAAS"
     gene            17976..18353
                     /locus_tag="VF633_12265"
     CDS             17976..18353
                     /locus_tag="VF633_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEX8471879.1"
                     /translation="MATTKAREDIKNDLKTLKEDLKTGAREEADRLKEKAAEAQERLK
                     AKSAATEARVREQADVLRDQARGYYDQARVRGREYYDDASERFDEAQRYITERVQERP
                     VQSTAIALGVGVVLGLLLAGRRR"
     gene            18353..18766
                     /locus_tag="VF633_12270"
     CDS             18353..18766
                     /locus_tag="VF633_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471880.1"
                     /translation="MIGKGLLKKVVASFAAAASAFVAVIAFGATIFCALCLIVPTLAA
                     AAITFGVFGILTALIAVIFLKPGSGHHDDGEEDEPESFAQKAVHLIRERPILGAAGGL
                     GILFFLLRNPALAAIAASMITEKRMESRGYGRRRR"
     gene            18892..19068
                     /locus_tag="VF633_12275"
     CDS             18892..19068
                     /locus_tag="VF633_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX8471881.1"
                     /translation="MLRWAIIFFIVALVAGVLGFGGIAGMSFEIAKFVAIIAVVLFLI
                     SLVAGGLRGRGTRI"
     gene            complement(19170..20027)
                     /locus_tag="VF633_12280"
     CDS             complement(19170..20027)
                     /locus_tag="VF633_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8471882.1"
                     /translation="MSTGRLIQFSDVHFGVEHQRACAAAIDYARTTPNDLILITGDVT
                     QKGLPKEFAAAGAWIRAMPEPRFVIVGNHDVPYYSALDRLFWPWKAFEAATGHPAHDG
                     EFKRDNLMVRGVTTARGWQARLNWSKGVIDLEQTRRAAAALRQAPVGALRILACHHPL
                     IEMIGTPMTGDVKRGDAAAGIFAEAGVDLIMTGHTHIPFALPIDLTDRCSYAVGCGTL
                     SHRERGTPPSFNQLEWDNKEIVVTAMAWCGKEFEPSQTWRLPRRQDTRDAATAPDPVV
                     AGEKERTVT"
     gene            complement(20041..21021)
                     /locus_tag="VF633_12285"
     CDS             complement(20041..21021)
                     /locus_tag="VF633_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8471883.1"
                     /translation="MTDTISRGTSEPDSAPVSNGAMAEAAQAVVRQLTPHIEIKRIMV
                     LVNPASGSVGPRASEEVQAILAEYPCEAEVVSLGGDIDAEIEAAIASKPDVLFVLAGD
                     GTARAVASRAGPKGPLVAPLPGGTMNMLPKALYGTADWKLALRAALEEGAPQSVSGGK
                     VEGQAFYCAAILGSPALWAPAREAMRSGKLSLAWAYGRRALRRAFTGRLRFSLDGGDK
                     GKTEALVLISPMISRALDEPVGLEAAAMDPSDTGQAFRLAATALFSDWRQDASVTTRP
                     ARRIDVWARSKIPAVIDGEPTLLPHETKVIFIPNAFRALAPNPPAAEDSV"
     gene            complement(21098..21466)
                     /locus_tag="VF633_12290"
     CDS             complement(21098..21466)
                     /locus_tag="VF633_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471884.1"
                     /translation="MRFPELVRNSDDTSHSPVWARNKRRRGRSGGSLVGLVVTLLALF
                     GALTAVMAIKERSIAAGGAVIDAWISAGADAVRQAIGRAPAAGAVAADKAGNAVEKTG
                     DALKAGAAETSDQMKSPTSR"
     gene            21654..22013
                     /locus_tag="VF633_12295"
     CDS             21654..22013
                     /locus_tag="VF633_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX8471885.1"
                     /translation="MKKALIAIGAAGLMASACASDPYGSSYGSTGSNQTVRQGAIGAG
                     LGAVAGAIIGNNVGGGNAATGALIGGLAGGAVGAIRGSNQDRSNQQRYRDNGGAYYYC
                     YDNRQSECYWENGQRRQ"
     gene            22137..22598
                     /locus_tag="VF633_12300"
     CDS             22137..22598
                     /locus_tag="VF633_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8471886.1"
                     /translation="MTNGRILVGVALLAGLGLAGCETSGRAFKDRSQIVETPTVCTPK
                     RFEIYFADSEARLTGPARQAIGLTATQLQGCNIKSVKVLGLSDARGGADANLDLSERR
                     AKTVAEALAAAGWPSPAFEVGAAGDEGAANASGVREPLRRRTEVLVDAAPN"
     gene            22660..24786
                     /locus_tag="VF633_12305"
     CDS             22660..24786
                     /locus_tag="VF633_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX8471887.1"
                     /translation="MRLFACILWLSLAFASGPAFAQAGPQKTERIEAELVPMSAWAAP
                     GSTAVVAIRQKIQPGWHTYWRNPGDSGGPTTLRWSLPTGVTAGDIVWPLPQRQRLQGL
                     TNFGYEGEVFLPVPIEVPASARTGTTIPLSVNVVFLVCSAEMCVPDQMTLTLNLPIKD
                     GAAPLAGAVGSRIQSVVETAPRPAGITAHATLNGQTLTLSATGGPLAGLTPKQASFFP
                     YEGDVIDHAAAQFGQRGASGVTLELAAGVDLKKTGLNGPISGVLSTDVGSWEITATPG
                     PALAGAGGSGALEPTPDAFGQAQGPISTGWLAVLQAAGFAFLGGVILNLMPCVFPILA
                     MKAASLTRSAHDPAEARRDGIAFTLGVLVAFAGLAAVLLILRAGGQAVGWGFQLQSPA
                     VVAALALLMLAVALNLSGVFEVGGRLQAVGGSRLTRLHGGTGAFFTGVLAVVVAAPCT
                     APFMAFALGAALVMPAPLAMIVFLMLGLGLALPYLLVSLSPSLLGRLPKPGHWMVTLQ
                     RWLAIPMYAAALWLVWVFWRQTALSPALLLVGACVVAAIGLAAAGVFAKRARWARGAG
                     CGAIAVAVVLTGVSAHLAAAAAATAPTEIPADAWSSQKVQTALAEGRPVLVNFTADWC
                     VSCKINERTALASPVTAKALKDANAVYLEGDWTRRDDAITRELQRHGRSGVPLYLLYT
                     PGQTEPRLLPQLLTEGVVVDALKTGK"
     gene            complement(24792..25697)
                     /locus_tag="VF633_12310"
     CDS             complement(24792..25697)
                     /locus_tag="VF633_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8471888.1"
                     /translation="MLPSERLKGIEAFVASADLGSFTAAGKRLNLTNSAISKSVGRLE
                     ARLSSRLFNRTTRSLKLTDAGTAFYATCVRVLGDLAEAEAVLTAHGVEPVGRVKLDLP
                     VAFGRMRVMPLLLKFAERYPAVRPQVSFTDRFVDVIEEGVDITVRIGGPEVWRDGIGH
                     RRLGAERLVFCAAPDWLAGQTMPKTPDDLIKLDGVLYGRSDGLTSPWRFGSAGGVEHR
                     AVEGRIVLGSAEAQVEAVKAGFGVAQLATWLVSRELESGELVELLPEYAVEGLPLHLI
                     WLTGRELSPKVAALLALFSEDLSVA"
     gene            25804..27012
                     /locus_tag="VF633_12315"
     CDS             25804..27012
                     /locus_tag="VF633_12315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8471889.1"
                     /translation="MSVTPISATPASPSAADKPRNGALSVLLLAISAFVIVTTEFIIV
                     GLLPALARDLNISITVAGMLVTLFAFSVMLFGPILTGLVSHFDRKRLFVVLLVIFAGS
                     NALAAVAPNIWVLALARFLPALALPIFWGTASETAGEIAGPERASKAVANVYLGIAGA
                     MVFGIPLGTLAGAAFGWRGAFWALAVLSLVMAVLLVAVMPTLAKPQRLRLGEQMKILK
                     SPFFLANIGLSVLVFTAMFTAYTYLADMLEQIARVPAKDVGWWLMGFGAIGLIGNWLG
                     GIMIGRGALRATALVSAVLAVGMAAAVPFAANPVALGLALAVWGLANTALYPICQIRV
                     MKAATHAQALAGTLNVSMANAGIGLGAIIGGAAIKSLGIGSLGYIAAGIAVLAVIAVP
                     FVARLQPKAA"
     gene            complement(27025..27330)
                     /locus_tag="VF633_12320"
     CDS             complement(27025..27330)
                     /locus_tag="VF633_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471890.1"
                     /translation="MIEESYVRLYAADFVRMAQSAETRPLDPTAVPKRMEQARSHATV
                     MDAAKGVGHLAALVTRLRDEARRPSQRHAAVLHSDATAVERRHLFLNTIADALSVPA"
     gene            complement(27609..32522)
                     /locus_tag="VF633_12325"
     CDS             complement(27609..32522)
                     /locus_tag="VF633_12325"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX8471891.1"
                     /translation="MSGDLRSVPQAISLEALEAAFEKAGGGGGPLVRAYLTQAHEDYA
                     ADETPELGGPDLAALLARVWTSADVRKTGEAARIVAQSLVAADGSGTGYDLVSIIQDD
                     RPFLVDSVMGELADAGVSVRALFHPVVTLGRDVSGARKTGDQRESMIVVIIDPLPAER
                     REALTTALAQTLADVQAAVSDHAAMHDLMERSVAHLEACPGTVDPAIVAENLEFLRWL
                     NADHFVFLGARDYDYPRTPDGGYAPEEPLGQASAGVGVLRDPERTVLRRTSEPAILTA
                     QMKRQIDMSDPVTVAKANLRSRVHRRAYMDFIGVKRYGDDGRPSGETRYVGLFTAEAY
                     DRAASEVPLVRRKVANALARAGKAPGSHNEKRLKNILENYPRDELFQISEDELLNIAL
                     GILHLYDRPRIKTFTRQDPFDRFISVLAFIPRERFDSSVRERIARILAAAWGGRLSAW
                     YPQLSDAPLVRIHYILGVTPGNHPTPDPVALESAVAEAGRSWIDRFESAARDADIDDA
                     EIGALSAKWAEAFGPGYRDRYDAAEAVADLQEIDRLNASGVKDGGEPVAVRAFRAVGD
                     NELQFRFKLYHRGAAVPLSDVLPILADMGLKTLEEFGHAIRPASDDAGDTEIHVHEFL
                     LEDPRGASLDFDDIKTPFETAFAAVWNGLTESDGFNRLVLELGVDWRQAALVRTLARY
                     RQQTGLDPSQAVQEEALRDYPDVARAIVSMFACKFDPDHGGPAEERAASTAELSDKIT
                     ALLQDVKSLDHDRALRRLALLVGAIKRTNYYQRDADGQPKPHISIKIASRELEDIPLP
                     KPFREIFVWAPHIEGVHLRFGPVARGGLRWSDRRDDFRTEVLGLVKAQQVKNAVIVPV
                     GSKGGFFPKYLSGIVRAGGDRDAQQAEAIRAYKTFLSGLLDITDNINGNGAVTRPDGI
                     VAYEGDDPYLVVAADKGTATFSDIANGVSADYGFWLGDAFASGGSVGYDHKAMGITAR
                     GAWEAVKRHFREIGKDIQSEPFTVVGVGDMSGDVFGNGLLLSKASKLIAAFDHRDIFI
                     DPTPDPAASWEERNRLFQLPRSSWQDYDKAKISEGGGVFSRGAKSIQLTPQIKAALDI
                     AEDTIDPVSLMRAILKAPAELLYLGGIGTYVKAATETDAQVGDKGTDALRIDAGELRV
                     KVVGEGANLGFTQAGRIEFARIGAGGSGGRINTDAIDNSAGVDTSDHEVNIKILIGSA
                     VTNGVLPADQRTALLASMTDEVGLKVLAHNYDQTLALALQQAEGAGALDAQQQFMQML
                     TARGRLDRKVEGLPSDLRINEMKSSGAALTRPELAVLMAYSKLELAEEIVASKAPEDP
                     FFEETLVRYFPEPLARFEEEMKSHRLRREIVATVLCNEIVNMAGPTFPDRLKASAEVD
                     TTAFVIAFEAARRVFRLDEAWDAVSALDLKIPAETQTALYTEISTVLRRQTFWLAKRA
                     GRDNVTVDSLIAAYRPAADALRQEGGDVLSRFEQDRLDKRLQKFAAMGVGEDLAKTIA
                     MLRPLVATADIGDLAKEAGWSEPAMARLYHQVGAAFDFDRLRAAAGGVPSNDHFDRLA
                     VRRLIEELMAEQVALTRAVARSTDASVGVSEEAAEAAVEKWIGPRQAVVESVRASVDE
                     IEASGSGWTFAKLTIANGAIRAVATSV"
     gene            complement(32714..33583)
                     /locus_tag="VF633_12330"
     CDS             complement(32714..33583)
                     /locus_tag="VF633_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8471892.1"
                     /translation="MKRTATLAAAASTAALLMAVAAPASAQTLFGQSGPESWTGPYVG
                     VFGGFTQQNGQDDERLRFDRNLDGNFGDTVTLATPAGADAFSPGFCDAGANSTTAAAG
                     CDKDGPGTQGGVRAGYDFQVGSFVVGVVGDYSAANQEDSVTGFSTTPASYTFTRTLNN
                     LAAVRARVGYAYGPALLYATGGVARGELENTFFTSNGANTFTSRVDEDEADGYQAGAG
                     VEYFLAPNLSVTGEYLYTSLDAGDNVIRVGQGSAAATNPFVLAPNTTGTDIIRSNSKF
                     GLHAFNVGMSYRF"
     gene            complement(33912..35111)
                     /locus_tag="VF633_12335"
     CDS             complement(33912..35111)
                     /locus_tag="VF633_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8471893.1"
                     /translation="MTPPISVATPTVSRNALVLIAVVTCAAALVWLADILTPLAMAIF
                     LMIVIDGVKRTIEEKTLLPQRWAGIASLVVVVLIFAGSIAIIIYGASGFFGEASGVAT
                     NIGPRIDQILRDGSNLVGVQQPPTLQTLINGIDVRGYLTAIALQAQNVASGAFFVMVY
                     LGFLLASQGGFRRKLIAMFPERLQRSEAMEVFQRVRSGVEGYIWVQSVTGAIICVFAW
                     ILMKAVGLQNAEFWTFVIFIVGFIPVLGGAIGGLAPPLFALVQFQTYWQAIVLLIGLQ
                     TVLFIVGNMIQPRMQGENQNIDPVAVLLSLALFGKMWGVVGMFLSTPLAVMAMAILAE
                     FKGSRWMAILLSGDGAPFADEEKKAAVAKTAESDEPSSRTAGSKPGHKRVKPPQVKPA
                     SGTDTGS"
     gene            35281..>36209
                     /locus_tag="VF633_12340"
     CDS             35281..>36209
                     /locus_tag="VF633_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX8471894.1"
                     /translation="MGQSNDSGAGVPEILLWQRANRHGVIAGATGTGKTVTLQILAQG
                     FSDAGVPVFCADVKGDLSGISQPGSPNEKLLARAAGMGLTLNPKAAPTVFWDLYGQKG
                     HPVRATVSEIGPLLMARMLDLNDVQEGVLTVAFHVADKEGLLLLDLDDLRSMLIYVAE
                     NAAKIGREVGNVAPASIASIQRALLQLEQDGGDAFFGEPALRLTDMMRTGLDGRGQVN
                     VLDSTRLMNSPRLYGAFLLWLLSELFEQLPEIGDPEKPKLVFFFDEAHLLFRDAPKPL
                     LEKVEQVVRLIRSKGVGIYFITQNPADIPDSVLA"
BASE COUNT         6073 a        11480 c        12459 g         6197 t
ORIGIN      
        1 cgaaccgcga ttgccagcca gatgttgggc acgaaggcca gggccaggta gccgatcgcg
       61 ttcgcggcca gggtgatcag caggacgggc ttgcgtccga tccggtccga cagccggccc
      121 cagaacggct cggcgaagaa ctggcccagc gaataggccg agaacatcag ggtgatctgc
      181 cacgcctcgg ccttcaggct ctgggcgaag aagggcagca gcggcaccac cagcccgaac
      241 ccgaccagat tgatgaacac caccagaaac agcaccgcca gcgcgggtgt tttgggctgg
      301 gtggctaagg ttggatcagg tctggccatc gtcatcgctt agccgcgtcc acgccgttcg
      361 ccaatcgtac agaggtttgt cgtcgagaac ctagtgtggc gggttgttcg tttcgaggaa
      421 ccggacggtc tcggtcagca tccgtattcg ggtttcgccc cgcgatagcc agtggtcctc
      481 gccgcttagt tcgataagct cgaccggctt tcctgcacga cgcagggctc ccgccatcga
      541 ccgactttgg tcgatcggaa ccacggtgtc gtcgcggccg tggatcagca gtatgggcgc
      601 atcagccttg tctgcctgac gcgccggcga tcgggcgtcc agtgaccggt ccccgggacc
      661 atcgccgccc atgaaccggt tccagtatcg aacggccccg ttgtcacgtc tctcgcctcg
      721 gctcgccgca tagttgacca tgcctcgcag gtcggaaact cccgcgacgg atacggcaca
      781 acgatagact ccgtgatcca gggttggtcc ggcgagggct gcgtagccgc cgtagcttgc
      841 cccgacgatg cagacgcgcc ccggatccgc gacgccctgt tcgacgagat gtcggacacc
      901 gtcagacaga tcggtctgca tcttgcgtcc ccactcgccg taaccggctt cgagaaagtc
      961 ctcgccgcgg cccgtggacc cgcggaaatt cgcctgaagg acggcatacc ctcgcgaagc
     1021 gagcgcctgg gcccaccaat cgaactcgcg cacatcgcgc gacgccggtc cgccgtgagc
     1081 gaggacgatc agaggcaggt tcttggcctc ccgcccgacc ggaacagtga gatagccgcg
     1141 aatttccaac ccgtcggcgg ctttataggc aaccagcgtg accggcgcca cctgatcggc
     1201 gggaaccgcc gaataggctg cgccaaccct caaagtcgag ctatcggtca ggtcgatgag
     1261 gtggtaggtg ccggaatcgt tggcgccgtt cgtgaacacg atcgcagtct tgaggtcatt
     1321 gctccaactg accagcgacg gcgcgccgtc gggaaaggtc tgcgcgacgc tggcccacag
     1381 gttgcctgcg tcctcatcag caaacgaata gcgccgtcct tcgtcgtcca gccgggtggc
     1441 gcccatcagc ctgcgagtca cggggtggaa cagcagatcc gtgggatcga actcgaaccg
     1501 gaccgcgcgc caggctcccg tcgccagatt gacgtcgtag tagtcggctt cttcgcgatc
     1561 gttgcgcttc agatccgggc gatcggcggc gacgatgacg ctttcacccc tcatgcccag
     1621 accgataata gcaggcgcat ccagcggcgc ggtgacccgc cagttctctt gaaaatcgct
     1681 accgtcacgc agcagcagtt tccagatctt cgtacggtcg tcaaagtcgc tgcgggcaag
     1741 cgatcgtccg ttcggatcca gcacatagtc gtcgacatcc cgatccatcg attcggcttc
     1801 gcgcatacgg ccggtctcgg catcgattct atagagattg atcttccccg gctcagtgaa
     1861 cgaaaaagcg cgcaccagaa catccggccg ggggccgggc ggggcgacat cgacgccgct
     1921 catcaacgcc gggaaaagtc cggaatcgcc ccggatcaag ccggtgaccc gcctccgtgc
     1981 cggatcgtag acctggccaa tgaagagttc gcggcggata atgcccaact caggcagact
     2041 ctccgtgctc gacgttgtga cgaggaccct gtcattcgag atccattgca agtcacggac
     2101 cttggacgcc ccaacaccga cgccacccat cgaatgattg gtcgcgaggt cgaccaaggc
     2161 gagggccctg gtctcgccga tgactgtgat aagcgccaga cgttggcccg acggcgacaa
     2221 ttgaacgagt tccagcgagg gcagggcgcc gtaagcggct agcggcggtg ggtcaccgac
     2281 gcccggcaat ggctcgtgtg cgtgggccag agacccggcc acgcacacga acagcgccag
     2341 agcggtcgct gcaacattct ggaaatacaa gattggcccc cgccgatcgc taacctgatc
     2401 actctcgacc ctgatcgccg caatctcaag tcgcaatctt tatgtggttt cgtctcgggc
     2461 gcggagttct gccgtctccc aatcttcgcc aaccccagac agcaaaaagc cccggtcgat
     2521 ctgaccgggg ctgtctggtg tcgttgattg gcgagccgcc tactttggcg cgaggatcat
     2581 gatcatctgg cggccttcca tacgcggtgc gtattcgacc ttggctatct cgtcgaaatc
     2641 ggcctggacc ttgttgagca gtttcatgcc cagttcgggg tgcgccattt cgcgaccacg
     2701 gaagcgcagg gtcaccttga ccttgtcacc ctcgtcgaag aagcggtgca tggccttggc
     2761 cttcacctca tagtcgtgga tgtcgatgtt ggggcggagc ttgatctctt tcagctcgac
     2821 gaccttctgg cgcttgcggg cctcggcctt cttcttctgc tcctggaagc ggaatttgcc
     2881 gtagtcgagg atcttggcca ccggcggttc cgacgtggac acgatctgca ccaggtccat
     2941 gcccgcttct tcggcggctt ccagcgcggc ggacgtcggc atgacgccct gcttctcgcc
     3001 gttctgatcg atgagcagaa cgcgcggcgc gcggatatcg tggttcatgg gcggcccatc
     3061 cttgacgggc ggcgtttgca tcggacggcg aatgagcgtc gtttccttgt gtgagcgaag
     3121 ggtcttgtcg ttttgcaggg gtcagcgcgc ggctcggcgc cgcgttcccc aagaggcgct
     3181 atatgcgccc gaaagcccgt tttatcaagg ttaggctgat cagggcttaa cgctgccgtc
     3241 gccagccgaa ttggccacga aatccgtgtg cagcgcgaca acggcgtcga acacgtgatc
     3301 gaccgtcagg tcctcgatcg gcgagaactc gccgcgtgtc tcattagacg cagcgatccg
     3361 ggtgcgaggg ccccacggcc cgtagagcca ccactcggtc ggtccgaaca gacccagcgt
     3421 cggacgtcca agcgccgccg agacgtgcat caagccggag tcattgccca cgaacagggt
     3481 cgcccgctcg attgcggccg ccgaggcgag gatgtcgccc ttgccgacga agtcgatgcc
     3541 gcgttcgcct gcggcctcca gcgcgggtgt cgcgggcgga cggtcaccgg ggccaccgac
     3601 ggccatgaaa cgccagccct cgaaacgtgg gtcggccttg agcttttcga ccagcgcgcc
     3661 ccagcgatcg gccggccagc tcttgcccgg ctgatgcgcg attggcgcca gagcaatgat
     3721 gggaccgggg ccggcggcgc ccgcgagttg cggatcgata acgcctgcgg cttcggatcg
     3781 cgcccggtcg tcgaggaaaa tctccggggc taacggcgtc tccgatccca tcagccgcga
     3841 gaccatctcg accttgcgca ggcccgtctc ccacgagcgg ttgtagatga cgcgccgctt
     3901 ggccgggatc agatagctga gcgccgagcc gcgaatgtcg atgaccatgt cccagcgcgt
     3961 cgtggcgacc tgcttccaca gatcgatcca gtgtccggcc agcttctttt tgtcgaggac
     4021 gatggcccgc tcgacgccgg gggcggaacg gaagaagggc gcaggcggcc ggccacaggc
     4081 gacggtgatc gccgcgcccg gtagttgacg ttcgatctcg cggatcacgc ccgacgagat
     4141 gacgcagtcg ccgatgcggt tggagctgac gaacaggact ttcggtacgg atgtgacggg
     4201 gggcacggat caaccttcgc ttcgttcctc gccgttcgct atcaggcgga gaccgtttgc
     4261 gcgagagtag gccgcatgac ccccaccgac cagcctgaga ccaacgacaa cgacatccag
     4321 catctggccc gcccggatgg agagaggctg gcctacaaac aggtccccgg cgaggggccg
     4381 accatcgtct ggatcggcgg cttccgttcc gacatggagg gaacgaaggc gctggcgctg
     4441 gacgccgccg cccgtgaacg tggttgggcc ttcctgcgct acgatcattt cgcccacggt
     4501 cagtcttccg gcgattggcg gcgggccacc atcgggcgct ggcgcgagga cgcggtcgcc
     4561 gtgctagaca gcttgaacgg ccctgccatt ctggttggat cgtccatggg cggttgggtc
     4621 gccttgctgg cggctctggc ccgacccgag gcggttgcgg ggctggtgct gatcaatccg
     4681 gcgcaggatt tcaccgagac cctgatgtgg ccgggcctcg ccgatcacga gcgtcaggcc
     4741 atcctgcgcg acggcgagac cgtggtcgtc gaagaaggat tgggttccta cgtcgtgacc
     4801 cgcgccatgt tcgaggaggc caggaactgg cttctgctcg gcggcccgat cccgattaag
     4861 gccatgaccc acatcctgca gggtcgcgcc gacgacaccg tgccgtggcg gtattcgatc
     4921 acactggtcg agcaattgac cggcggcgat gtcatgctgg atctgatcga gggcggcgat
     4981 caccgcttgt cgcaaccggc tgacctgacc cggatgatca acgccattga tgccatgcgc
     5041 aaagcctgag cttctcaccg gatctcaggc gggtatggtg acgctcatgg acactctatc
     5101 cgagcgctgg gcgaagcttg aaccgaccac caacgtcgtg ggtccggatg acgatctgaa
     5161 gcgaccggcg gtgctgctgt tccatggctg cggcggcatg cgcccgcatc ttccgcttta
     5221 tgcagcggcg gcgaaggcgg cgggatggcg agccttcatc gtcgattcat acgccccgcg
     5281 cggctggggt cgggtcttcg ccctctccat ggtctgcaca ggcgccatcc tgagaggaaa
     5341 cgaacgtggc ggcgacgtgc tggcggccat cgacggcgtt tcgcgacgct cggacgtcga
     5401 ttccggcaag atcgccgtgg ctggctggag ccatggcggc tggagcatca tggaaatgct
     5461 ggcggccccg cgcacccaca gcggcgaact gggcatcgcc gatccctcca tcgtctgcct
     5521 ctcggggctg aaaggcgcat ggatgggcta tccctacgtc ggcttcctcg cggtgggacg
     5581 taaccgcccc tggaggcatc atccaaagac cgaggtgatg atcgccaaac acgaccacct
     5641 gaccaccgtg cgcaatgccg agcaggtgtt cgagggccat cgtggtcgag gcacggtcat
     5701 cgagacctgg gttgccgacg gcacccattc gttcgacgaa cccactagcg ctccgccgtc
     5761 gcgacatgat ccggcgctga ccgaggtcgc cgtgaagcga ttcggccagt tcctgatcgg
     5821 gctctagccg attgaggctt cgactaggat ttcggcgcgg accgagtggg ccatttcgca
     5881 gtgctcatgc gcggagtgat gcagggcatc cagttcgccc tgcgtcgggg ccacgccgaa
     5941 gaaggtcgtg acaggccgca gggtcacctt gtccatataa cgccgaccct tatcgtcacg
     6001 cccaagggtc ccggaggcct cgtcgagata gcggtcgacg accagtccgt cgccggcggc
     6061 gaaggcgagg aaccatagca tgtggcagct cgacagcgag gcgaccatgg cctcctcggg
     6121 gtccacagcc gtcacgtcgg acatcggaac cggcacgctc tcgggcgccg aagatccctg
     6181 caccaccgcc ccgccgtcga aagtccagac gtgggcgcga gagtaggcct tgtcgatgaa
     6241 ggcctgaccg tcgcgcttcc attcgacggt ggtgcggtgc gcgctcatgc ttctaagccc
     6301 gccagaagcc gacgatcttc ttgacctcgt cgacgaccgg gtcggctatg gcggcggcgc
     6361 gttcggcgcc gtccttgagg actcggtcga tctcggcggt gtcagccatc aggcggcgca
     6421 tctcggcggt gatcggggcc atggcctcga cggcgaggtc ggcgaggcgc ggcttgaagg
     6481 cgccgaagcc ctgaccggcg aactcggccg tgacggcgtc cttcgtctgg tcggacaggg
     6541 ctgcgtagat ggcgatcagg ttcttgacct cgggccggtc gtccagaacg tcgccgacct
     6601 caggcatgac gcccatgtcc gtcttggatt tgcggatctt gccggcgatg gtatcggcgt
     6661 catcggtgag gttgatgcgc gactggtccg acggatccga tttcgacatc ttcgctgcac
     6721 cgtcccgcag ggacatgacg cggggcgccg gcccctgcgt caggggttcg ggcaggggaa
     6781 agaagccggg cacgccgaag tcgttgttga acttctgggc gatgtcgcgg gtcagttcca
     6841 gatgctgttt ctggtcctcg ccggtcggca cctcggtcgc cttgtagagc aggatgtcag
     6901 ccgcctgcag caccgggtag gtatagaggc cgacgctcga ccgttcctta tgcttgcccg
     6961 acttctcctt gaactgggtc atccggtcga gccagccgag ccgggcgacg cagttgaaga
     7021 tccacgccag ttcggcatgc gcacggaccg ccgattgcgg gaagatcacc gacttcgccg
     7081 ggtcgagacc cgaggccaga taggcggcgg cgatctcgcg cgtctgggcg gtcagcagag
     7141 ccggatcctg ccagacggtg atggcgtgca ggtcggccac gaacaggaag cagggcgcgc
     7201 ccccatcctg caactcggtg aagcgtttga gggctccgag atagttgccc aaatgcaggg
     7261 cgcccgaggc ctggatgccg gaaagcaccc ggcgcgggcc ggtgtaggcg gggagggtcg
     7321 gcgtagcatc ggacatcagc ggcagatgcc ccagccacgc tgcgagagca agttcctact
     7381 ccagcccgcc acccgatgcc gcggcgcccg gttcgcggcg caagacggcc ttgagttcgg
     7441 acggtgtgac cgcgccgaac aggaacaggc agaagcaata gatgaccgcg ccgacgccgc
     7501 agaccacgat gacggcgatc tccttggcga ggaacagctt gctgagctcg ggatagaaga
     7561 tgcctgccgc cagcagcagc gccgccatca ctccgctggc cgccagcacc cgcatgatgc
     7621 gcgagacgaa gttggctgac ggcctccacg tgttttctcg tatgagggtt ccgatgagca
     7681 gggcgacgtt gatccaggcc gaggtgctgg tggcgatggc cagacccagc acgccgtcga
     7741 tgccgatgtg cgagaaccag tagaacaggc tcgcgcccag cacgacggtc accagcaccg
     7801 aggccacggc gaagatcatc gggcgacgcg tgtcctcccg agcgaagaac ggcggcgtca
     7861 ggaccttggc gaggacgaag gcgggcacgc cccaggcgaa ctggcgcaaa acgtcggcgg
     7921 tgcgcgcggc gtccagcgag gtgaaggcgc cgcgagtgac ggtggcgtcg atgatgaaga
     7981 aggggatgac gaacagggcc acggcggcgg gcagggtgaa ggccatcgac agcgtcaggc
     8041 cgtcgtccag tgtccggcgt ccgccctcgt gatcgcccga gacgaaagcc ttggtcagcc
     8101 ggggcacgag ggcgagaccg atggcgacac cgacgaggcc tagcggcagt tgatacaggc
     8161 ggtcggcatt atagagtacc gaacgcgcgc ccggattgga cccggtcagg aactggctga
     8221 cgacggagtt gatctgcagg gcgccgccgg cgagcacgcc gggaatggcg agggcgagcg
     8281 tcttcttcac cccggcggta aggcgcggcc atgacagctt cagcatgacg ccgagacgac
     8341 gtacgcccca ccacaacagg cccgcctgaa gcacacccga gatcgtgacg gcgatgctga
     8401 cggcatcgag gatgtgcggc tggctgatcg tcatgaactc gggcgccagc agcgggacga
     8461 gagtgcaaag gttcaggaag accgggacgc ccgccgacag ggcgaacctg ccgctggtgt
     8521 tcagcacgcc cgacagcagg gacgcgatgg tcatgcaggc gaggtagggc atcgtcagct
     8581 gggctgcggt gacggcggcg aacatcacgc ctgaatcgtt gcgataggcg gacaacagca
     8641 ccggcatgat ccacggcatg gccagttgca ggacgatgca gaaacccgcg accacggcga
     8701 acatgaagct cagggcctcg gacgcggtga cggctgcggc ttcctcgccg tctcgggcgc
     8761 ggacgccgcc gtagatcggc acgaacgctt gggcgaaggc gccctcggcg aacaggcggc
     8821 ggaacatgtt cggcagcatg agcgcggtgg tgaaggcgtc catcatcggc ccctggccga
     8881 accgcgccga cagcagcagg tcgcgcccga aacccagaag gcggctgccc agcgtcaggc
     8941 cggactgaac gatcgtattg cgagccaggc tcatgcaggg aggtccgggc cgctggaacc
     9001 tgcggcgaaa tgtgcgctta gcgcgcttcc ggctcaggcg aaacggcctt tgccttgcgg
     9061 agcgggcgat tgagatcgga gacgtcgcga gcactggccc gggcggcgac gatggcgcgt
     9121 cctttcggag cggccggggt gcgttccaac accgcctcga gaccgtgctg gatctcgttc
     9181 agcagcagtt ccgagaacac cttccggccc ttgcgatcca ccacgtaaaa actgtcaacg
     9241 gcccgttcgc cgaagcccgc gacgtgggcc gagcggatcg acaggtcgag atcgctcagg
     9301 gtccgggaca gctcggccag aaggccgggc cggtcggcgc cggagacctc gatcaccgtg
     9361 gcgctctcac tggcgccggc gtcgatcatg accacggggc gaacgtcgaa aacggcccgc
     9421 cggggcgtgg cgataggcat ggccggcgca tcgatgggcg cctcgccccg tgcagcagcc
     9481 tccagcgcgg cgacgagagt cttcaggcgt cgcggttcgg cttggccata gggggcgtcc
     9541 gcgccgtcct gaacctgaaa cacgtccagc accgtgccgt cggcggcagt ggcgacccgc
     9601 gccccggtga cgtcggcgcc ggcggcggcg aggacggcgg tcaggtcggc gaacaggccg
     9661 ggccggtcgc gcgccgccaa ggcgatctcg gtggtcgcct cgatccgccc tggcccctgc
     9721 cgacactcgg cggccgcgcc ggtttcgcag gcgcgggcga tcagatgggc gtgttccgcc
     9781 aaaggatcga tcagatcgac atcttcaccc cggaacaggg cttcgacgcg ggtatagagg
     9841 gcggtgatca actgaccctt ccagttgttc cagacgcccg gcccgacggc gcggatgtcg
     9901 gcgacggtca gcaccaacag cagccgcagc cgctcgggat caccgacgat ctcggcgaag
     9961 tcgcggatcg tctgggggtc ggaggtgtcg cgcttctggg catagtcgga cagcgcgagg
    10021 tggttgcgaa ccagccagac gaccagctcg atccggcgcg gctcgacgcc cagccgttcg
    10081 caagcccgac gcgcggcgat ggccccgtcc tcgagctggc ctcgttcgcc acccttgccg
    10141 acgtcatgca gcagcatggc caacatcagc acctccagat cggcgatcag gtggatggtc
    10201 gaggacgaca gcggatggtc ttccttcaac ttgccccgga agatgtcgtt gatgatgccg
    10261 atggcctgca ggctgtgctc gtcgacggta taggcgtggt acatgttgaa ctgggtctgg
    10321 ccgacgatcc ggccccattc gggcaggaac cggcccagca ggcccgtctc gttcatgatg
    10381 gtgaggacgc gatagggcct ctgcccgtgc gccagaatgt gcagcagggc ggcggtggcg
    10441 cgagggtccc gtctcagctt cggcgtcacc agacccaacg accgcgtcac tgccgagaag
    10501 gcgtgcgggt ggacgtcgag gtcgtgctga tcggcgatga cgaacaggga cagcagcttg
    10561 accggatcat cggcgaacac ctgcgcgctc tccaccgaca gccgcccgcc gtcctcgatg
    10621 aagccggcga cgccgaggtt gcgcttccgc cccgggatga gccgcgaaag gctcatcgtc
    10681 ttcttctgct ggcgcgcctc cagcgtcgcg ctcatcgcgc gggtcagggc gccgacgtca
    10741 cgagcgacga ggaagtatcg acgcatgaac cgctcgactg cgggctcgtc gccccgaccg
    10801 cgccagccca ttcgacgggc gacttcgggc tggagatcga aggtcagctt ttcctcggcg
    10861 cgccctgcgg tcaggtgcag atgtgtccgc acccgccaca ggaagtcgaa cgcctcctcg
    10921 aacgtccggc gctcgcggga agtcagcagg tcgtcgagca cggccgcgcc gagcgggctc
    10981 tcgggcgcca gagctctggc aatccagaac agggtgttga ggtctcgcag accgcccttg
    11041 ccgtccttga tgttgggctc gacgcggtaa cggacggcgc ccgccttggt gtgccgggct
    11101 tcgcgttctt ccagcttggc ggcgatgaac gggcgcggat cggcgttcgt cacggtcccc
    11161 cggaagcgcc agaggaaatc ctcggccaga ggctggtctc ccgccagcca gcggccttcc
    11221 agcaaggcgg tgcggacggt catgtcgctg cgggagaggg ccagacattc atctaccgac
    11281 cgggccgacg acccgacctt gagccccagg tcccacagga catagagaac gaactccgcc
    11341 accgtctcgg tgcgggccgt ggtcttccac gggcgcagga acagcaggtc gagatccgag
    11401 aacggcgcca gcacgccacg tccgtacccg ccgaccgcga tcagggtcag cttctcggcc
    11461 tcggtcggat tgtgcgatgg atagagggtc tcagtggtga accgccacag ggcgatcagg
    11521 atgtcgtcgg ctgcggcgga atactgacgc gccacctcga ccccgccgag accggcggcc
    11581 agacgtaagg cgatcttgtc ccgcgccgcg gcgtaatggc tccgcagcag tgcggcgacg
    11641 ccggcgcgaa cgtccgaacc gccgacgaga gcatccaggc ggtcatccag acctgttcgg
    11701 gaagatgtca gggaaacggg ggtcatcggc ttgcgcaata tagtcacgac gaagccctgc
    11761 gtctcgttca gggtttggta cgcgcactgt gtctccggcg ccgcgtccgc tcgccaacgc
    11821 ggggagtgcg ctggagtctc tgccaaggtt gaggcagggt tccggaacac tgttcccgga
    11881 gccttcttga taccgcgtct ggtcctcgtc gcagctctgg cagtcatggc gcttagcctt
    11941 ggcctgtttt cgcatgcgtg ggccggggag ggtccggagg acggtttggg cgcgagtgat
    12001 ggatcccgta gtggctctgc ccttggcgcc gatcgcagcg aattgcgtcg cgagcactgg
    12061 tccgacggcc tgaccatggc ggcagccgga tacggcccgg tcggaggtga tgcccagctg
    12121 tcgcgcgagg tcctgggcga gatgaaccgg atgcgttcgg atccgcaggc ctatgtcgcc
    12181 gtgctgcagg actaccgccg tcagtttcag ggcgccatcg ttgtccgtcc gggccgcatc
    12241 aacatccaga cccgcgaggg cgtagccgca gtcgacgagg ccatcgcctt cctgcggcgt
    12301 cagaagccgg tgtcgcggct ttcgcccgat ttgatcctgg ccctgacggc tggagatcat
    12361 gtggccgacc agggcccgac cggctttatc ggccactacg gttctgacgg gtgggatttc
    12421 gtgacccggg tgtcgcggcg tgggggcgat ccctacggcg gcgagaacat ttcctacggc
    12481 tacgataccg cgcgcgaggt catggtccag ctgctggtcg acgacaatat cgcggacagg
    12541 ggtcaccgga ccaacctgtt ccgcgacggc tatgtccgcg ctggcgtgag ctgcggccct
    12601 cacgcgctgt tcaaccacat gtgcgtcatc gactacggct atgagcctgt caaaggcagg
    12661 taggtagtca ggtcgtaggc cttgggagcg tcggatcccc tgcgggtatg cttcctgtct
    12721 gctgcctact tcctcttcaa agccttcagg cgatacagcg cctcaagcgc ctcgcggggg
    12781 ctcatcgcat ccggatcaag ggcggcgacg gcttcgtcca tggcggactt catcggcggg
    12841 ggcggcggtt ctgccaaggc gaacaggggc aggtcgtcga gccggtgatt ggcggccttt
    12901 tcgctttcca gacgatcgag taccgagcgg gctcgcgcaa cgacggacgg cggcactccc
    12961 gccagtttgg ctacctgaac gccataggat cggtcggcag cgccgggcgc ggcctcgtgc
    13021 aggaagacta gctcgccatt ccactccttg gctttcatcg acaggttgga gacgtggtcg
    13081 agccgctctt cgagccgcgc cagctcgtga taatgagtgg cgaacagcgt ccgggcgcgg
    13141 ttggtgtcat gcagggcttc ggcgcaggcc caggcgatgg cgagaccgtc ataggtcgac
    13201 gtgccgcgcc cgatctcatc cagcaccacg aaactggcgg gggtcgcctg ggtcaggatg
    13261 gcggcggtct cgaccatctc catcatgaag gtggagcggc cgcgcgcgag gtcgtcgcca
    13321 gcgccgacgc ggctgaacag tcgatcgacc acgcccagcc gcatcgaccg cgcgggcacg
    13381 aaggatccgg cctgggccag gatcaccagc agcgcattct ggcgcaggaa cgtcgatttg
    13441 cccgccatgt tgggcccggt gacgatcgaa agacgcggac agcccccgcc cttgatcggc
    13501 gcgccgtcca gtcggcagtc gttcggcgta aagggatcgc cggccttctt gaccgccgcc
    13561 tcgaccaccg gatgccgtcc ggcctgcacc tcgaacgtca ggctggagtc gacggtcggg
    13621 cgcaccgccc cgacctcctc ggcccattcc gcgagggccg aggtggcgtc cagttcggcc
    13681 agcgcgtcgg cgaccgcctg cagagggcga gccagccgct ggacggtctg gcgccagcct
    13741 tcgaaggtct cgccctccat ggcgagcgcg cgataacccg cctggctgat tttcgcgtcg
    13801 agttccgaca gttcgacggt ggtgaaccgg acctgcgccg ccagagtctg gcggtggatg
    13861 tagggactct ctggaccgac acggaacagc ggctcggcgt gtttggcggt ggtctcgagg
    13921 aaatagccga gcaccgcgtt gtgcttgatc ttgaaaggca ccccactgtc ggcgacggcg
    13981 cgggcctcaa gatcggcgac aacccggcgg ctgtcgtccc tcagggtgcg ggcggcgtcc
    14041 agctcgggtc ggaaaccggg acggacaaag ccgccgtcgc gcgccagatg cggcggctct
    14101 tccatcagcc cttcggccag atcgaccatc agctgggcgg tgtccggcga cagggtcagc
    14161 cggtcgagac agccgatgat ccgcgcaggc ggagcgctca atgggttgcc tgagacgacg
    14221 aacaagccgg cgatgccctc ggcgatggtc aggcctgagc ggatggcggc gaggtcgcgc
    14281 ggaccgccac gccccaacgc caaccgtgac acagcgcgcg ccacgtcggc ggaggctttc
    14341 agcccgtcgc gcaggtcgcg gcgcaggtcg cggcgctcca gcaaccattc gacggcgtca
    14401 agtccatcat tgatcgcgac gggatcacgg agcggccggg atatccgctc cgccagcgcc
    14461 cgcgctccgc ccgaggtcac agtgcgatcg agacaggcga gcagcgaccc ctcgcgttcg
    14521 ccacgctggg tgcgatcgat ctcaaggctc tggcgggtgg cgggatcgat ggcgaggaag
    14581 ccgctctcgc cggaccggcg cggcggtgtc agggccggga ccttgcccgc ctgagtggtc
    14641 tccagatagg ccgcgatcag gcccagcgcg gagacctcgg cctcctcgaa ggcgccgaac
    14701 ccgtccagcg aggccacgcc gtacaagcgt tcgacacgat cgcgcgccgc agcgggctcg
    14761 gcgacggccg aggccatggc ctgaactacg ccgcccgacc cgtcgagcgc cggtcgcact
    14821 gcatcgtctg agaacagctt gtccggaacc agcacttcgg acggccggaa cgcggccagg
    14881 gtcgcagcga gatcgcccag atcgcaggcg atggaatcca ccgctccggc agacagctcg
    14941 accacggcga ccgcggcgcg gcccttgcgg atggcgacgg cggcgagccg gttcgctcca
    15001 cgcgcttcca gcagcgagtc ctcggtcaga gtgccgggcg tgacgacccg aacaatgtcg
    15061 cgcgcgacga tggatttgga gccgcgcttt cgggcctcgg cgggcgtctc gatctggtcg
    15121 cagatcgcga cccgttgccc tgcccggatc agccgcgcca gatatccgtc cagcgcgtgg
    15181 accggcaccc cggccatcgg gatgtcctcg ccctgatgct tgccgcgctt ggtcagggtg
    15241 atgccgagca gacccgaggc gatctcggcg tccttgaaga acagctcgta gaaatcgccc
    15301 atgcggaaga agacgatggc gtcgggctgc gccgccttgg ccgccagaaa ctgggccatg
    15361 acgggcgtca cgccgtcagt cgaaactggc gtcgaggggg actgcggagg atgtgcgagg
    15421 ggagcgttca tgcgaagcgc acagggtgac ggatcgcggg ccccgtctca agccattccc
    15481 ggataacggg ccgaaagtac ggggctcagg ccgcgtggct gatcgggatg cgcggtccgg
    15541 ccgtcagggc ggcaggttcg gtcagggcca gcgcgtccca gtcgagatcg ggcttggggg
    15601 cgcaggacgc cgcgaggatg gcgcgaagtt ccgccgcaga ggcgacgctg gcgaccacgc
    15661 aggtcgcggg cgggaggctg agcgcgaagg caagcgcggc ctgcatcggg tcgacccgag
    15721 cctcggccag acggcggcga acgcgcgaca gcgccacgcc atgctcggcc agatgggggg
    15781 gcaggctgtc gcggctggcg aatagcaggc cacgtgcgaa cacggacgac aggtgaacgt
    15841 cgatgttcat tgccgccaga tccgacagaa cgccttcgct gaccgcgcgc tgatccagaa
    15901 gattgcaggg catctgcacc acgtcgggct ggtaccggcg cgccagcagg gcgggaccat
    15961 cctccatggt cgcgcagaag ccgatccggc gatacaggcc gcgatccttg agtgatttca
    16021 tccgatccca aagggcgcga ccctcggccc ccgccagatc ggcagcgttg gaaaccagca
    16081 gaacatctcc gcgcgggagg cccagacgct ccagcgaacg gcgggcgcga gcctcgaccc
    16141 gatcaaggcc atcagccaga ggcacagtgc gcacggtgac gcggaagggg gaggggaacg
    16201 gccaggcctg acccagcaaa cgttctccgt cgcggtcggg acgtgtggcc acgaccttga
    16261 tgccggcgtc tgccgccgtc tggagcagga agcgcatggc gtcttcccgt gcgccgacgg
    16321 gagccgtcgt cacggcgccg acggcccgtt cgggctcggt caccaccgcc atggcgagtt
    16381 tgccggcggg agtctgtgcg ggaagcggct tcacccgccg accttgcacg acaatgttta
    16441 agggaggctg acccggaagc ttacagagtc ttacctgcag accgctggtc gactgaagtt
    16501 tccgtggaaa atcagtccgc gtctggctgg atatcaggac gggcggcgac aaaggcggaa
    16561 tgagtctcgg ctgcctgctc tactgccagc agttttggcc aaggcgtgag atcgaggttg
    16621 aagcgtcttg ccgaatagag ctgcgggatc agataacagt cggccagcgt gggcgtggcc
    16681 ccgaagctcc agcccttccc gtactgggcc accatctgct caagggcgtc aaacccggcc
    16741 ttgatccagc cgcccgccca ggcgtcaacc ccgttctgat cggcccccag acccctcacc
    16801 gcccccagca cgcgcagatt gttcagtgga tgaatatcgc tgccgatgag ggcggccatg
    16861 gccctgacac gggcgcgctc cagaggtccg gcggggagga gcgccggttc aggataggcg
    16921 tcctccagcc actcgaggat cgctacgctc tgcgtcagga tcgcaccgtc tatttcgagc
    16981 gcaggcacca tcccctgtgg attcagcgcc agatattccg gcgtcttgtg cgcgcgctga
    17041 cgcagatcga ccgtttgttg ttcgtaggcc agccccttca ggttcagggc gatccgggtc
    17101 cggtacgacg ttccggaccg ccagtaggaa tgcaggatca cgacgcggcg gtttccgcga
    17161 cggtgaagac cagcgtcgtc aggccgtcga tgctcgcctc gacgcggtcg ccgggaacca
    17221 gcggtccaac gccctcgggc gtgccggtga agatcaggtc gccgggctgc agcgtccaga
    17281 gttctccggc cttggcgacg atctcgtgcg ggttccagat catgtccgac aggtcgcccg
    17341 cctgttttgc caccccgttc accgccaggg tgatggaggc gcagggttcg ggcaggtcgc
    17401 ctagcgtgat cgggccgcag ggcgccgact ggtcaaagcc cttcgaggcg tcccaaggcc
    17461 ggcctttttc ctttgctgca gcttgaaggt cgcgtcgtgt cagatcgacg ccaacggccc
    17521 agccaatggc gccgatgctt ccttcgctgg tcgacccgat cgcgacaacc agctcgacct
    17581 cgtggtgcag attggtcgtg gcggagggat aggccggatc cttgccgtcg gtgacgacgg
    17641 cgtcagccgg cttggagaaa aagaagggcg ccggtctcga ggggtcgccc cccatctcgc
    17701 gcacatgggc ggcgtagttc tggcccacgc aaaggatgcg acgcacggga aatcggcgtc
    17761 ctgcctgtgc cggctcgccg acgatcggca gggacggggt aggtgcgggg gggaacagcc
    17821 agtcggtcat gagcgggttc taggacggcc agagggctgc gtcatctgtc ctgcgtgctg
    17881 cgctgcggcg aaagaagccg gaactctcat gacactgtag cgttcagcgg ctgaggcatc
    17941 tatttaaagc aaagcttatc accggagcgc gcgacatggc cacgaccaag gctcgagaag
    18001 acatcaagaa cgacctgaag accctcaagg aagacctgaa gaccggcgcc cgcgaggaag
    18061 cggaccgtct gaaggaaaag gccgccgagg ctcaggaacg tctgaaagca aagtcggccg
    18121 ccacggaggc ccgggtgcgc gagcaggctg acgtcctgcg cgatcaagcc cgtggctact
    18181 acgatcaggc tcgcgtgcgc ggtcgggaat actatgacga cgcctccgag cgtttcgatg
    18241 aagcccaacg ctacatcacg gaacgggtgc aggagcgtcc ggtccagtcc accgctatcg
    18301 cgctcggcgt gggtgtggtg cttggtcttc ttctggccgg tcgtcgccgc tgatgatcgg
    18361 caaggggctg cttaaaaaag tggtcgcaag ctttgccgct gcggccagtg ctttcgttgc
    18421 ggtgatcgcg ttcggcgcga ctattttctg cgctctttgc ctgatcgtgc ccacgttggc
    18481 tgcggcggcg attacgttcg gcgtgttcgg aatcctgacg gccttgatag ccgtcatttt
    18541 cctgaaacca ggcagtggtc atcatgatga cggcgaggaa gacgagccgg aaagcttcgc
    18601 tcagaaggcg gtgcacctca tccgtgagcg tccgatactc ggcgctgctg gcgggctcgg
    18661 catcctgttc ttcctgctgc ggaatccggc gctggccgcg atagccgcca gcatgatcac
    18721 cgaaaaacgg atggagagcc gaggctacgg tcgtcgccgc agatagctaa gcagatgtat
    18781 tggcccgacg gaacggtgcg aggtgagcgg cgttgataat ttgtgcggcg ccggtcaggc
    18841 ggcgtccgac ttgatcaacc gtttcaaccc gttcctgagc tggaggctat catgctgcgt
    18901 tgggctatca tcttcttcat cgtggcgctc gtcgccggcg ttctgggctt cggcggcatc
    18961 gccggcatgt cgttcgagat cgcaaaattc gtcgctatca tcgcggtcgt cctgttcctg
    19021 atctccctcg ttgcgggcgg tcttcgcgga cgcggtacga ggatataatc aggcgcttcg
    19081 gcgcttggcg aagaaaccgc cgtccgaagg ggcggcggtt ttttgtgcgt gcgatatggt
    19141 ccgtaacggg aagtcggaat gcatgaagcc taggtgaccg tcctctcctt ctcacctgcg
    19201 acaacggggt cgggcgcggt cgctgcatcc cgcgtgtcct gtcgacgagg caggcgccag
    19261 gtctggctgg gctcgaactc tttcccgcac caggccatcg ccgtgacaac gatctccttg
    19321 ttatcccatt ccagctgatt gaagctaggc ggcgtgccgc gctcgcgatg cgacagcgtc
    19381 ccgcagccca ccgcgtaaga gcatcggtcc gtcagatcga tcggcagggc gaatggaata
    19441 tgggtgtggc cggtcatgat cagatcgact ccggcctcgg cgaatatccc ggcggcggcg
    19501 tcaccccgtt tgacgtcgcc cgtcatgggc gtgccgatca tttctatcag gggatgatga
    19561 caggcgagta ttctcagcgc cccgacggga gcctgccgca gggccgccgc ggcacggcgc
    19621 gtctgctcca gatcgatcac gcctttcgac cagttgaggc gggcttgcca gccgcgagcg
    19681 gtcgtcacgc cccgcaccat caggttgtca cgcttgaact cgccgtcgtg agccggatgg
    19741 ccggtcgcag cctcgaaagc tttccatggc cagaacagcc ggtccaacgc actataatag
    19801 ggaacgtcgt ggtttccgac gatgacgaac cggggctccg gcatcgcgcg tatccaggcg
    19861 ccggccgccg caaactcttt gggcaggccc ttttgggtca cgtcgccggt aatcaggatc
    19921 aggtcgttag gcgtcgtccg ggcgtagtcg atcgccgcag cgcaggcgcg ttggtgctcc
    19981 accccgaaat ggacgtcgga aaattggatc aggcgtccgg tgctcaaggc ggcgttctct
    20041 tcatacgctg tcttccgccg ccggcgggtt tggggcaagc gcccggaagg cgttggggat
    20101 gaagatcacc ttggtctcgt gcggaagcaa ggtcggctcg ccgtctataa ctgccgggat
    20161 tttcgagcgc gcccagacgt cgatccgtcg ggcgggtctg gtggtcacgg atgcatcctg
    20221 acgccagtcg ctgaacaagg cggtagccgc cagcctgaag gcttgcccgg tatcggacgg
    20281 gtccatggct gccgcctcca gccctacggg ctcgtccagt gcgcgggaga tcattgggct
    20341 gatcaggacg agcgcctcgg tcttgccctt gtcgcctccg tccagcgaaa atctcagccg
    20401 ccctgtgaag gcgcggcgca atgcccgacg gccataagcc caggccaatg ataatttgcc
    20461 cgatcgcatc gcctcccgcg ccggagccca gagcgcgggc gagcccagga tcgcggcgca
    20521 atagaacgcc tggccctcga ccttgccgcc cgatacggat tgcggggcgc cctcttccag
    20581 tgctgccctg agcgccagtt tccagtcggc ggtgccgtag agtgccttcg gcagcatgtt
    20641 catggtgccg ccgggcaggg gcgccacgag gggtcccttc ggacctgccc gtgacgcgac
    20701 ggcccgcgcc gtgccgtcgc ctgcgagaac gaaaaggacg tcgggcttcg aagcgatggc
    20761 cgcctcgatc tcggcgtcga tatcccctcc gagcgacacc acctccgctt cgcaaggata
    20821 ttcggccaga atggcctgga cctcttcgga cgcgcggggg ccgacggacc ccgacgcggg
    20881 attcaccagc accatgatcc gcttgatctc gatatgaggc gtcaactggc ggacgaccgc
    20941 ttgcgcagcc tcggccatcg ccccgttaga gacgggcgca ctgtcgggct cagaggtccc
    21001 gcggctgatc gtgtcggtca tcacgcccga acgtcccgct gcaactgacg ttccgatacc
    21061 aagcgtatct attctaaaag gacgtgatgc agcgcgctta tcgggaggtg ggcgacttca
    21121 tctgatccga ggtctctgca gcgccggcct tgagagcgtc gccggttttc tccacggcgt
    21181 ttccagcctt gtcggcggcc acagcccccg cggcaggcgc cctgcctatg gcttgccgca
    21241 ccgcatcggc gcccgccgaa atccaggcgt cgatgacggc gcctccggca gcgatcgagc
    21301 gttccttgat ggccattacg gcggtcaggg ctccgaacaa ggcaagaagg gtaaccacaa
    21361 gtccgaccag cgagcccccg ctccggccac gacggcgttt gttgcgcgcc cagacagggc
    21421 tgtggctcgt atcgtccgag ttccggacta gttcaggaaa acgcatgtca gaccccccta
    21481 acaattttat tgtaacgccg tacgacgcga agccgtgccg gcagttctgg tagaatgttt
    21541 acgaaccgaa tatcggggga acctgtgcca taatccagcg ttctccccct gggggcgaac
    21601 aatcggcccg ctttcggctc attgcgggcc atccgggatg aaggaataca cccatgaaaa
    21661 aagcacttat cgccatcgga gccgccggtc tcatggcttc ggcctgcgcg agcgaccctt
    21721 acggcagctc gtatggcagc accggctcta accagaccgt ccgccaaggc gccatcggcg
    21781 caggcctcgg cgccgtcgcc ggcgccatta tcggcaacaa cgtgggcggc ggcaacgcag
    21841 ccaccggcgc cctgatcggc ggcctcgccg gcggcgctgt cggcgcaatc cgcggttcga
    21901 accaggaccg ttcgaaccag cagcgctacc gcgacaacgg cggcgcgtac tactattgct
    21961 acgataaccg tcaatcggaa tgctattggg aaaacggcca acgccgccag taggactctc
    22021 cggccgtccg tgggacggta ggactgattc gggggcggtt cgctgtttgc ggaccgcccc
    22081 ttttcgtgtc ggggcgagat ttttggaacg tcggttttgc gacgcagggg tagacgatga
    22141 cgaacggcag gatcttggtc ggggtggctc tgctggcagg gctggggctg gcggggtgcg
    22201 agacatccgg gcgagcgttc aaggaccggt cacagatcgt cgagacgccg acggtctgta
    22261 cgccaaagcg attcgaaatc tattttgccg atagcgaggc gcggctgacg gggccggcgc
    22321 ggcaggcgat cgggctgacg gcgacgcagt tgcaaggctg caatatcaag agcgtcaagg
    22381 tgctggggct gtcggatgcg cgcggtggcg ctgacgccaa tctggacttg tccgagcgac
    22441 gcgccaagac ggttgccgag gcgcttgcgg cggctgggtg gccctcgccg gcgtttgaag
    22501 tgggtgcggc cggcgatgag ggtgcggcga acgcatcggg cgtgcgtgag ccgttgcggc
    22561 ggcgcaccga ggtgctcgtc gacgcagccc cgaattagaa ctccgaccag gaggcgtcag
    22621 aggctatagg tcgggcccca acggtccgga ccttcgcctt tgcgcctttt cgcctgcatc
    22681 ctctggctga gcctggcttt tgcttcgggc cctgcgttcg cgcaggcggg tccgcagaag
    22741 accgagcgga tcgaggcgga actggtcccg atgtcggcct gggccgcgcc gggatcgacg
    22801 gcggtcgtgg cgatccggca gaagattcag ccgggctggc atacctactg gcgcaatccc
    22861 ggcgactccg gcggaccgac gacgttgcgc tggagtctgc cgacgggtgt cacggcgggc
    22921 gatatcgtct ggcctttgcc ccagcgccag cggctgcaag gcctgaccaa ttttgggtat
    22981 gagggcgagg tcttcctgcc ggtgccgatc gaggttccgg cctcggcgag gaccgggacg
    23041 accattccct tgagcgtcaa cgtcgtgttc ctcgtctgca gtgccgaaat gtgcgtgccg
    23101 gaccagatga ccctgacgct gaacctgccg atcaaggacg gcgctgcgcc tctggcggga
    23161 gccgtgggta gtcgcatcca gagcgtggtc gagacggctc cgcgtccggc ggggatcacc
    23221 gcccacgcga ccctgaacgg ccagacactg acgctcagcg ccaccggcgg cccgttggcg
    23281 ggtctcacgc cgaaacaggc gtccttcttc ccttacgagg gtgatgtcat cgaccacgcg
    23341 gcggcgcagt tcggccagcg tggagcctct ggcgtgacgc tggaactggc ggcgggcgtg
    23401 gacctgaaga agacaggctt gaacggcccg atctccggcg tcctttcgac cgacgtcggc
    23461 agttgggaga tcacggcgac gcccggcccc gcgcttgcgg gcgcaggcgg gtcgggggct
    23521 ttggaaccca cgccggacgc gtttggccaa gcgcagggtc caatatcgac gggctggctg
    23581 gcggtgcttc aggctgcggg gtttgctttt ctcggaggcg tgatcctgaa cctgatgccg
    23641 tgtgtcttcc cgatattggc gatgaaggcg gcgtccctta cccgttcggc acacgatccg
    23701 gcagaggccc gtcgcgacgg gatagccttc acgctcggcg tgctcgttgc gttcgccgga
    23761 ctggctgcgg tgctgctgat cctgcgggcc ggaggtcagg ccgtggggtg gggcttccag
    23821 ctccagtctc ctgcggtagt ggctgctctg gccctgctga tgctcgccgt ggcgttgaac
    23881 ctgtcgggcg tgttcgaggt cggtggtcgg ctgcaggcgg taggcggctc gcgcctgacc
    23941 cggctgcacg gcggcacggg cgccttcttc actggcgtgc tggctgtcgt ggtggctgcg
    24001 ccttgcaccg cgccgttcat ggccttcgcg ctgggagctg cgctggtgat gcccgcgccg
    24061 ctggcgatga tcgtcttcct gatgctgggc ctcggactgg cgctgcccta tctgctcgtc
    24121 agcctttcgc cgagcctgct gggacgactg cccaagccgg gtcactggat ggtgaccctg
    24181 cagcgctggt tggccatacc gatgtacgcc gccgccctgt ggttggtctg ggtcttctgg
    24241 aggcagaccg cactgtcccc cgccctcttg ctggtcggcg cgtgtgtggt ggcggcgatc
    24301 ggactggccg ccgccggcgt cttcgccaaa cgcgcccgat gggcgcgcgg cgcgggctgt
    24361 ggcgccatcg cggtcgccgt cgttctgacc ggcgtgtcgg cgcacctcgc agcggcagcg
    24421 gcagcgaccg ccccgaccga gatccccgcc gacgcctggt cctcgcagaa ggtgcagacg
    24481 gctctggccg agggccgtcc ggtgctggtc aatttcaccg ccgactggtg cgtcagctgc
    24541 aagatcaacg agcgcacggc gctggcctca ccggttacgg ccaaggcgct caaggacgcc
    24601 aacgccgtct atctggaagg cgactggacc cggcgcgatg acgcgatcac gcgtgaactc
    24661 cagcgccacg gacggtcagg ggtgccgctg tacctgctct acacaccggg ccagaccgag
    24721 ccgcgattgt tgcctcagct gctgactgag ggcgtcgtgg tggatgcgct gaagaccggc
    24781 aagtaagcag ctcaggcgac gctgaggtct tcgctgaaga gcgccagaag agcggcgacc
    24841 ttgggactga gctcccggcc ggtcagccag atgagatgca gcggcagccc ctctaccgcg
    24901 tactcgggaa gcaattcgac cagctctccg ctctcgagtt cgcgactgac cagccatgtt
    24961 gcgagttggg cgacgccgaa gccggccttg accgcctcga cctgcgcctc tgcactgccg
    25021 agaacgatac gaccctcgac cgcccggtgc tcgacgccac ctgcgctgcc gaagcgccag
    25081 ggtgaggtca ggccgtcgct gcggccatag agcacgccgt ccaacttgat caggtcgtcc
    25141 ggagttttcg gcatggtctg tccggcgagc caatccggcg cggcgcagaa gaccagacgc
    25201 tcggccccca gccggcgatg gccgatgccg tcgcgccaga cctcgggacc cccgatcctc
    25261 accgtgatgt cgacgccttc ctcgataaca tcgacgaagc gatcggtgaa cgagacctgc
    25321 ggccgaaccg ccggatagcg ctcggcgaat ttgagcagca gcggcataac gcgcatccgt
    25381 ccgaacgcca ccggcaggtc cagtttgacg cgccccacgg gctcgacgcc atgggctgta
    25441 agcaccgctt cggcctcggc gaggtcgccg agcacgcgca cgcaggtggc gtagaaggcg
    25501 gtcccggcgt cggtcagttt caggctgcga gtggtgcggt taaacagtcg cgatgacagg
    25561 cgggcctcca gccgccccac gctcttgcta atcgcggagt tggtcaggtt gagacgcttt
    25621 ccagcagcgg tgaagctgcc gaggtcggcg gaggcgacga aggcctctat tcccttgagc
    25681 cgttcggacg ggagcatcag tgacttccaa ctctggacaa aaattccata atagctggaa
    25741 atctgaccgc gtgtacggat cgatcgtcaa tgatagatga tctgcctctc tttggaggcc
    25801 tccgtgtccg tcacccccat ttccgctaca cccgcctcgc caagcgctgc cgacaagccg
    25861 cgcaacggcg cgctctcggt gctcctgctg gccatctcgg cgttcgtcat cgtgaccacc
    25921 gagttcatca tcgtgggcct gctgccggcc ttggcgcggg acctgaacat cagcatcacg
    25981 gtcgccggga tgctggtcac gctgttcgcc ttcagcgtca tgctgttcgg accgatcctg
    26041 accgggctgg tctcacattt cgaccgcaag cggctgttcg tcgtgctgct ggtcatcttc
    26101 gccggctcga atgcgctggc ggccgtcgcg ccgaacatct gggtgctggc gctggcccgg
    26161 ttcctgcccg cgctggccct gccgatcttc tggggcacgg ccagtgagac cgccggtgag
    26221 atcgctggac cggagcgcgc cagcaaggcc gtcgccaacg tctatctggg catcgccggg
    26281 gccatggtgt tcggcattcc gctcggcacg ttggcggggg ccgcgttcgg ctggcgaggc
    26341 gccttctggg ctctggcggt gctgtcgctg gtgatggccg tgcttctggt ggctgtcatg
    26401 ccgacgctgg ccaagcctca gcgcctgcgt ctgggcgagc agatgaaaat cctgaagtcg
    26461 ccgttcttcc tcgccaatat cggcctgtcg gtgcttgtct tcaccgcaat gttcaccgcc
    26521 tacacctacc tcgccgacat gctggagcag atcgcgcgag tgcctgcgaa ggatgtcggc
    26581 tggtggttga tgggctttgg cgccatcggc ctgatcggca actggcttgg cggcatcatg
    26641 atcgggcgtg gggccctgcg cgccacggcg ctggtctcgg cggtgctggc ggtcgggatg
    26701 gctgcggcgg ttccgttcgc tgcaaacccg gtagccctcg gcctggcgct ggccgtttgg
    26761 ggtctggcca acaccgcgct ctacccgatc tgccagattc gcgtgatgaa ggccgcgacc
    26821 catgcgcagg cgctggcggg cacgctgaac gtgtcgatgg ccaatgcggg cattggtctg
    26881 ggcgcgatca tcggtggtgc agcgatcaag tcgctgggca tcggcagcct cggctacatc
    26941 gccgccggta ttgcggtgct ggctgtgatc gccgttccgt tcgtcgcgcg tctgcagccg
    27001 aaagcggcct gaggcgaacg cctgtcaggc cggaacgctg agggcgtcgg cgatggtgtt
    27061 gaggaacagg tggcggcgtt ccacagccgt ggcgtccgag tgcaggactg ccgcgtgacg
    27121 ctgtgacggg cgacgggctt cgtcccgcag acgcgtaacc agcgcggcga ggtgacccac
    27181 gcccttggcc gcgtccatta ccgtggcgtg cgagcgggcc tgttccatgc gcttgggcac
    27241 ggcggtcggg tcgaggggcc gggtctcggc gctctgggcc atgcggacga aatcggcagc
    27301 gtagagccgg acgtagcttt cctcgatcat gcggactttc cttgaagaca tgacgcgctg
    27361 tcgtgcagcg cggggcgctg ttgggtcggc cggacagacg gagtcatgga gcgatcgttt
    27421 cgcaggggga tcaaaccggg gcggatcgac cgccacaggg gcggctcgct ctcggcgacc
    27481 atgcgttgat ataggcgtga tcggacaaaa ttgcgagcgg aagctcgccg ggaggccggc
    27541 ggaggggtga cgtaggggag tccgtcaccc ctccggcccg ccttgcgagg ggacggaggg
    27601 gcttgggatc agaccgaggt ggcgacggcg cggatggcgc cgttggcgat ggtcagtttg
    27661 gcgaaggtcc agcccgatcc ggaggcctcg atttcgtcga ccgaggcacg gacgctttcg
    27721 accacggcct gacgcgggcc gatccatttt tcgacagcgg cctcggcggc ttcctcgctg
    27781 acccccacgg aggcgtcggt gctcctggcc acggcgcggg tcagggcgac ctgttcggcc
    27841 atcagttcct cgatcagacg gcggacggca agacgatcga aatggtcgtt cgacggaacg
    27901 ccgcccgccg ccgctcgcag ccggtcgaaa tcgaaggcgg cgccgacctg gtgatacagg
    27961 cgcgccatcg cgggttcgga ccaaccggct tccttggcca gatcgccgat atcggcggtg
    28021 gcgacgaggg gacgcagcat cgcgatggtt ttcgccagat cctcgcccac gcccatcgcg
    28081 gcgaacttct gcagccgctt gtcgagacgg tcctgctcga agcgggacag gacgtcgccg
    28141 ccctcctgac gcagggcgtc tgcggccggg cggtaggcgg cgatcaggct gtcgaccgtg
    28201 acattgtcgc gacccgcccg cttggccagc cagaaggtct ggcggcgcag cacggtcgag
    28261 atttcggtgt agagcgcggt ctgggtctcg gcgggaatct tgaggtccag cgcactgacg
    28321 gcgtcccagg cctcgtcgag gcggaagacc cggcgggcgg cctcgaaggc gatgacgaag
    28381 gccgtggtgt cgacctcggc cgaggccttg agacggtccg ggaaggtcgg gcctgccatg
    28441 ttgacgatct cgttgcagag caccgtggcg acgatctcgc gacgcagtcg gtggctcttc
    28501 atctcttcct cgaaccgggc gagcggctcg gggaagtagc ggaccagcgt ctcctcgaag
    28561 aagggatcct cgggcgcctt cgaggccaca atctcctcgg ccagctccag cttggaatag
    28621 gccatcagca cggccagctc gggccgggtc agggccgcac cggacgactt catctcgttg
    28681 atgcgcaggt cgctgggcag accctcgacc ttgcggtcca gtctgcctct ggcggtcagc
    28741 atctgcatga actgctgctg ggcgtcgagc gcgccggccc cttcggcctg ttgcagggcc
    28801 agggcgaggg tctggtcgta gttgtgggcc agcaccttga ggccgacttc atccgtcatc
    28861 gaggccagca gcgccgtgcg ttgatcggca ggcaggacgc cgttggtcac cgccgagccg
    28921 atcaggatct tgatgttgac ctcgtggtcg gaggtgtcga ccccggccga gttgtcgatg
    28981 gcgtcggtgt tgatgcggcc cccggagccg cctgcgccga tccgcgcgaa ctcgatccgg
    29041 cccgcctggg tgaagccgag attggcgcct tcgcccacga ccttgacccg aagctcgccc
    29101 gcgtcgatgc gcagcgcgtc ggtgcccttg tcgccgacct gggcgtcggt ctccgtcgcg
    29161 gccttcacat aggtgccgat gccgccgaga tagagcagtt cggcgggggc cttgaggatg
    29221 gcgcgcatca ggctgacggg atcgatcgtg tcctcggcga tgtcgagggc tgccttgatc
    29281 tggggcgtca gttggatcga cttggcgccg cgcgagaaga cgccgccgcc ttccgagatc
    29341 ttcgccttgt cgtagtcctg ccaggacgag cgcggcagct ggaacaggcg gttgcgctct
    29401 tcccatgagg cggcgggatc aggcgtcgga tcgatgaaga tgtcgcggtg gtcgaaggcg
    29461 gcgatcagct tggacgcctt ggacagcagc aggccgttgc cgaaaacgtc gcccgacatg
    29521 tcgcccacgc cgacgacggt gaagggctcg gactggatgt ccttgccgat ctcgcggaag
    29581 tggcgcttga ccgcctccca cgcgccgcgg gcggtgatgc ccatggcctt gtggtcgtag
    29641 ccgacggatc cgcccgaagc gaaagcatcg ccgagccaga aaccgtagtc ggccgagacg
    29701 ccgttggcga tgtcggagaa ggtcgctgtg cccttgtcgg cggcgacgac cagataggga
    29761 tcgtctccct cataggcgac gatgccgtcg ggtcgcgtca cggcgccgtt gccgttgatg
    29821 ttgtcggtga tgtccagcag gccggacagg aaggtcttgt aggcgcggat ggcctcggcc
    29881 tgttgcgcgt cgcgatcccc gcccgcgcgg acgatgccgg acaggtattt ggggaagaag
    29941 ccgcccttgg agccgaccgg gacgatgacc gcgttcttga cctgctgcgc cttcaccaga
    30001 ccgaggacct cggtgcggaa gtcgtcgcga cggtcggacc aacgcaggcc gccgcgtgcc
    30061 acggggccga accgcaggtg gacgccttcg atgtgcggag cccagacgaa gatttcgcgg
    30121 aagggcttgg gcagggggat gtcttcgagc tcgcgcgagg cgatcttgat cgagatgtgc
    30181 ggcttgggct gaccatcggc gtcgcgctga tagtagttgg tgcgtttgat ggccccgacc
    30241 agcagggcga ggcggcgcag ggcccggtcg tggtcgaggc tcttcacgtc ctgcaggagg
    30301 gccgtgatct tgtcggacag ttcggcggta gaggccgcgc gttcctcggc gggcccgcca
    30361 tgatcgggat cgaatttgca ggcgaacatc gagacgatgg cgcgggcgac gtcgggatag
    30421 tcgcgcaggg cctcctcctg caccgcctgc gacggatcga gaccggtctg ctggcgatag
    30481 cgggccaagg tgcggaccag agccgcctga cgccagtcga cgcccagctc cagcaccagc
    30541 cggttgaagc cgtcgctctc ggtcaggccg ttccagacgg cggcgaaggc ggtctcgaac
    30601 ggcgtcttga tgtcgtcgaa gtcgaggctg gcgccgcgcg ggtcttccag caggaactcg
    30661 tggacgtgga tctcggtgtc gccggcgtcg tcagaggcgg ggcggatggc gtggccgaac
    30721 tcttccagcg tcttgaggcc catgtcggca aggatcggca ggacgtcgga gagcggcacg
    30781 gcggctccac gatggtagag cttgaagcgg aactgcagct cattgtcgcc tacggcgcgg
    30841 aaggcgcgca cggcgacggg ttcaccccca tccttcacgc cagatgcgtt gaggcggtca
    30901 atttcctgca gatcggcgac ggcctcggcg gcgtcgtagc ggtcgcgata gcctggcccg
    30961 aaggcctcgg cccatttggc cgacagggcg ccgatctcgg cgtcgtcgat atccgcgtcg
    31021 cgggcggcgc tttcgaaacg gtcgatccag ctgcggccgg cctcggccac ggcggactcc
    31081 agggcgactg gatcgggcgt cgggtgattg cccggcgtga ccccgagaat gtagtggatg
    31141 cgcacgaggg gggcgtccga cagctgcgga taccaggccg acagacggcc gccccatgcg
    31201 gcggcgagga tgcgggcgat ccgctcgcgg acgctggaat cgaaacgctc gcgcgggata
    31261 aaggcgagga ccgagatgaa ccggtcgaac gggtcctgac gcgtgaaggt tttgatgcgc
    31321 ggccggtcgt agaggtggag aatgccaagc gcgatgttga gcagttcgtc ctcggatatc
    31381 tggaagagct cgtcgcgcgg atagttttcg aggatgtttt tgaggcgttt ttcgttgtga
    31441 ctgcccgggg ccttgcctgc acgggcgagg gcgttggcga ccttgcggcg caccagcggc
    31501 acctccgagg cggcgcggtc ataggcctcg gcggtgaaca ggccgacgta gcgggtctcg
    31561 cccgatggac gaccgtcgtc gccgtagcgt ttgaccccga tgaaatccat ataggcgcgg
    31621 cggtggacgc ggctgcgaag gttggccttg gcgacggtga ccggatccga catgtcgatc
    31681 tgacgtttca tctgcgccgt cagaattgcg ggttcggagg tgcggcgcag gacggtgcgt
    31741 tccgggtccc gcaggacgcc gacaccggcg ctggcctgac caagcggctc ctcaggggca
    31801 tagccgccgt ccggagtacg cgggtaatcg tagtcgcggg cgccgaggaa aacgaaatgg
    31861 tccgcgttga gccagcgcag gaactcgagg ttttcggcga cgatcgccgg atcgacggtg
    31921 ccgggacagg cttccagatg ggcgaccgaa cgttccatga gatcatgcat tgccgcgtga
    31981 tcggaaaccg cggcctggac gtcggcgagg gtttgggcaa gggctgtcgt cagcgcctcg
    32041 cgacgctcgg ctggaagcgg atcgatgatc acgacgatca tggactcgcg ttggtcaccg
    32101 gtcttgcggg cccccgacac gtcacgtcca agcgtcacta ccgggtgaaa cagggcccgg
    32161 accgagacgc cggcgtcggc gagttcgccc atgacgctgt cgacgaggaa tggacggtcg
    32221 tcctggatga tcgaaacgag gtcgtaaccg gtgccggacc catcagcggc gacaagggac
    32281 tgggcgacaa ttcgggccgc ctcgccggtc tttcgcacat cggcggaggt ccagacccgc
    32341 gcgaggaggg cggcgagatc cggcccgccc agttcgggcg tttcatctgc ggcgtagtct
    32401 tcgtgagctt gcgtcagata ggcgcggacc aagggcccgc cgccccctcc tgccttttcg
    32461 aacgcggctt cgagcgcctc cagggaaatt gcctgaggga cggagcgaag gtcgccagac
    32521 atgggcaggg tctcgtgcag cgcggccggg agccgcaagg gatcaccgac attaggccgt
    32581 cggcgcttag gatcaatgaa tggctgatta ggcccgaccg gcgccctctg gaagccgtaa
    32641 acgacaacgc cggacccgct ttcgcggacc cggcgtccgt tatcattgat aagtttgtcc
    32701 gaggatcggc ggactagaag cggtagctca tgccgacgtt gaaggcatgc aggccaaact
    32761 tcgagttcga gcggatgatg tccgtgccgg tcgtgttggg agccagaacg aaggggttgg
    32821 tcgccgcggc cgagccctga ccaacgcgga tcacgttatc gcccgcgtcg agcgaggtgt
    32881 agaggtactc gcctgtgacg ctgagattcg gggcgaggaa atattcgacg ccggcgcccg
    32941 cctgatagcc gtccgcctcg tcttcatcga cgcgcgaggt gaaggtgttg gcgccgttcg
    33001 aggtgaagaa ggtgttttcc agttcaccgc gagccacgcc gcccgtcgca tagagcagag
    33061 ccggaccgta ggcgtagccg acgcgcgcgc ggacggctgc gaggttattc agggtgcggg
    33121 tgaaggtgta ggatgctggg gtcgtcgaga agccggtgac gctgtcttcc tgattggctg
    33181 cgctgtagtc gccgaccacg ccgacgacga aggacccgac ctggaagtcg taaccggcgc
    33241 ggacgccgcc ctgggttccc gggccgtcct tgtcacagcc ggcagcggcg gtggtggagt
    33301 tggcgccggc atcgcagaac cccggcgaga aggcgtcggc gccggccgga gtggcgagcg
    33361 tgacggtgtc gccgaagttt ccgtcgaggt tgcggtcgaa gcgcaggcgc tcgtcgtctt
    33421 ggccgttctg ctgggtgaag ccgccgaaga cgccgacgta ggggccggtc cagctttcag
    33481 ggccggattg accaaacagg gtctgggcgg aagccggggc ggcgacggcc atcagaagag
    33541 cagcggtgga ggcagcggcg gcgagtgtag cggttcgttt catggggagg gtcctttgcg
    33601 aatgggtgag cgaatcccgc ctcatctcga gcggcgttcg cggtcctaac cccgcagata
    33661 cgaaacggga tgcattcggg atgaggcttg tgccaatcag tggcactttt acatcagcgg
    33721 ttttgtcgtt cggaaacaac gctcttccga agcggaaaaa caaggccgcc ggacgggggg
    33781 tccgacggcc tgctttctcg gggtcgacgc tggaggggaa tagagcgccg tccccggatc
    33841 gacatggacc ctggaggggg gaggggaggt ccgcgtcgtc tgagttcaag ataagaaggc
    33901 gttgtggcgg ttcaagaccc ggtatcggtg cccgatgccg gtttgacctg tggcggcttg
    33961 acgcgcttgt ggccgggctt cgacccggcc gttcgcgaac tcggctcgtc gctttcagcc
    34021 gtcttcgcca cagcggcttt cttttcctcg tcggcaaagg gggctccgtc gcccgacagc
    34081 aggatagcca tccacctcga gcccttgaac tcggccagga tcgccatggc catgaccgcg
    34141 agaggcgtcg acaggaacat gcccaccacg ccccacatct tgccgaagag ggccagcgac
    34201 agcaggaccg caaccggatc gatgttctgg ttctcgccct gcatgcgcgg ctgaatcatg
    34261 ttgccgacga tgaagagcac cgtctgcagg ccgatcagga ggacaatcgc ctgccagtag
    34321 gtctggaact gtacgagcgc gaacagcggc ggagccaggc ccccgatcgc gccgcccaga
    34381 accggaatga agccgacgat gaagatgacg aaggtccaga actcggcgtt ctgaagaccc
    34441 acggccttca tcaggatcca cgcgaacacg cagatgatcg caccggtgac ggactgaacc
    34501 cagatgtagc cctcaacccc gctgcggaca cgctggaaca cttccatggc ttcggagcgc
    34561 tgcagtcgtt cggggaacat cgcgatcagc ttgcgcctga agccgccctg cgacgcgagg
    34621 aggaagccta gatagaccat gacgaagaac gcgcccgagg cgacgttctg ggcctgcaat
    34681 gcgatggcgg tgagatagcc ccgaacgtcg atgccgttga tgagtgtctg cagggtcggt
    34741 ggttgttgaa cgcccacgag gttcgaacca tcgcgcagaa tctggtcgat acgtggcccg
    34801 atgttggtcg cgacgcccga agcctcgccg aagaagccgg atgccccata gatgatgatc
    34861 gcgatggagc cggcaaagat caaaaccacc acgacaagcg aggctatgcc cgcccagcgt
    34921 tgcggcagga gcgtcttttc ctcgatggtc cgcttcacgc cgtcgatgac gatcatcagg
    34981 aagatggcca tcgccagcgg cgtcaggatg tccgccaacc agaccagagc ggcagcgcac
    35041 gtcactaccg cgatcagaac gagggcgttg cgagagacgg ttggcgtggc gacggagata
    35101 ggtggtgtca ttcggggact ttcaggtgcg gcagacgccg cgtcaacgta gatcgggggc
    35161 gatccgtgaa gcttctgtcg ccaacgcttt aacggcgcga acggctgcgc tactccggta
    35221 aatcactgga gccgccccat gcccgatgtc gtcacctcta cccagaccgc tggcctgttc
    35281 atgggccagt ccaatgacag cggcgcaggc gtgcctgaaa tcctgctgtg gcagcgcgcc
    35341 aaccggcatg gggtcatcgc cggggccacc ggtacaggca agaccgtgac gctccagatt
    35401 ttggcccagg gcttctccga cgccggtgtg ccggtcttct gcgccgacgt taagggcgac
    35461 ctttcaggca tcagtcagcc gggctcgccg aacgaaaagc ttctggcccg cgccgcaggc
    35521 atggggctaa cgctcaaccc gaaggccgca ccgacggtgt tctgggacct gtatggccag
    35581 aaaggccatc cggtccgggc gacggtcagt gaaatcgggc cgttgctgat ggcgcggatg
    35641 ctggatctga acgacgtgca ggagggtgtt ctgacggtcg ccttccacgt cgcggacaag
    35701 gagggcttgc tgctgctgga tctcgacgac ctgcgctcga tgctgatcta tgtggccgag
    35761 aacgcggcga agatcggccg ggaagtcggc aatgtggctc cggcctcgat cgcctcgatc
    35821 cagcgcgcct tgcttcagct tgaacaggac ggcggcgacg ccttcttcgg cgagccggcg
    35881 ctgcggctga cggacatgat gcgcacaggt ctggacgggc ggggtcaggt caatgtgctg
    35941 gactcgaccc ggctgatgaa cagcccgcgt ctttatgggg cgttcctgct gtggctgctg
    36001 tcggaactgt tcgagcaact gcccgagatc ggcgaccccg agaagccgaa gctggtcttc
    36061 ttcttcgacg aggctcacct gctgttccgc gatgcgccca agcctttgct ggagaaagtc
    36121 gagcaggtgg tgcggctgat ccgatccaag ggcgtgggca tctatttcat cacccagaac
    36181 ccggctgaca ttcccgacag cgtgctggc
//
LOCUS       DASVTM010000449         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_523587,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000449 DASVTM010000000
VERSION     DASVTM010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523587"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VF633_12345"
     CDS             <1..1386
                     /locus_tag="VF633_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471895.1"
                     /translation="AAAPSVQTSSQAVVAGPAHVSVLRDGDKWTADYRLNGDAPVWAF
                     QRSALLRVGRTPWRPAWWSVETPGVVLERQGRYDVLRSLDGGPVPREVRIVMHPQAGD
                     LEADYDPALIFTDGTVALYSGQFDVMPLPSVAVARELPLDLNGIDLPNSDPAEVRWHD
                     AAGPVLFKGERLDAPVAIDADTYVLFGDAQIRQNSGITTVIDPGLPRWLGDELGQFTP
                     RVMEFYERRLGESRGPAPTLMVSWTGPTEKISSMGGSVLPGLISMNFEGEGVLDPDPA
                     ALSRAHWFIGHESAHFWLGSNGLKYEFARDAWITEGGADLMAVRAIQAIDPTYNARAE
                     LQTEVDDCVKLAAGKPVATAGERGENRAFYACGGVWSMALEAARAGRSGGDFLDVVAD
                     FQRDAGADGTLTREEWLSALTRAARDPSLRISIERMLDVGVHDPAAIIGRLFDRTGVA
                     YRLEAGKIVLS"
     gene            complement(1409..>2202)
                     /locus_tag="VF633_12350"
     CDS             complement(1409..>2202)
                     /locus_tag="VF633_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX8471896.1"
                     /translation="RLLLSETLGPSSRAFAVIYQLVLAEHAVELAAMVDAADMPDSIS
                     RDLLKVSLTNALVAAVLMPYGAFQRAAEQTGYDIARLQARFGVSFEQAAHRLTTLSRP
                     TGRGVPFFLMRVDHAGNVSKRYASAGFPFSRFGGACPRWRLHSAFRTPGRIITQIIET
                     PPQNGKPGGRWFTLARTVDRQGHDAFSEGGDLAIGLGCELRHAHRLIYARGLDLQNPE
                     VTPIGPACRLCHIHPCAERAAPPIDRALTVDDWSKSVSAYPFSAV"
BASE COUNT          368 a          664 c          795 g          375 t
ORIGIN      
        1 gccgccgcgc catcggtcca gacatcctcg caggcagtgg tcgcggggcc ggcccacgtg
       61 tctgtgctgc gtgacggtga caagtggacg gcggattatc gcctgaacgg cgacgccccc
      121 gtctgggctt ttcagcgctc cgccttgttg cgggttgggc ggacgccctg gagacctgcg
      181 tggtggtctg ttgagacgcc cggtgtcgtg ctggagcgcc aggggcgcta cgacgtgctg
      241 agaagcctgg acggcggtcc ggttccgcgt gaggtgcgca tcgtcatgca tccgcaagcc
      301 ggagacctcg aagccgacta tgaccccgca ctgatcttca ccgacgggac ggtcgcgctc
      361 tactccggcc agttcgacgt aatgcctctg ccgtcggtcg cggtggcgcg cgaactgccg
      421 ctggacctga atgggatcga cctgcccaac agcgatcctg ccgaggtgcg ctggcacgac
      481 gcagcagggc cggtgctgtt caagggcgag cggctcgatg ccccggtggc gatcgacgct
      541 gacacctacg tgttgttcgg cgatgcgcag atccggcaga actctggcat caccaccgtg
      601 attgatcccg gtttgccgcg ctggctcggc gacgaactcg gtcagtttac gccgcgggtg
      661 atggaattct acgaacggcg tctgggtgag agccgaggtc cggcgccgac cttgatggtc
      721 agttggaccg gtccgaccga gaagatttcc agcatgggcg gcagcgttct gcccgggctg
      781 atctcgatga atttcgaagg ggagggcgtg ctggaccccg atcccgccgc cctgtcgcgc
      841 gcccactggt tcatcggcca cgagtcggcg catttctggc tcgggtcgaa cgggctcaaa
      901 tatgagttcg cccgggacgc ctggatcacc gagggtgggg ccgacctgat ggccgtacgc
      961 gccatccagg ccattgaccc gacctataac gcccgcgccg aacttcagac cgaggtcgac
     1021 gactgcgtaa agctggcggc aggaaagcct gtggcgacgg cgggcgagcg cggcgagaac
     1081 cgggccttct acgcctgcgg cggggtctgg agcatggcgc ttgaagctgc gcgggcaggg
     1141 cgcagcggtg gcgattttct cgatgtagtc gccgacttcc agcgcgacgc gggagccgac
     1201 ggcaccctga cgcgcgagga atggctctcg gccctgaccc gcgccgcccg tgatccgtcg
     1261 ctgaggatca gcatcgagcg gatgctggac gtcggcgtgc atgatccggc cgcgatcatc
     1321 ggtcggctgt ttgaccggac gggtgtggcg taccggctgg aggcggggaa gatcgtgctg
     1381 agttagccac catccgggga ttggcgagtt agaccgctga gaacggatag gcgctgaccg
     1441 acttcgacca gtcgtcgacc gtgagggcgc ggtctatcgg cggagcggcg cgttcggcgc
     1501 agggatggat gtggcacagg cggcaagcgg ggccgatggg ggtaacctca gggttctgga
     1561 gatcgagacc ccgggcgtag ataagccggt gcgcgtgtct gagttcgcag ccgaggccga
     1621 tggcgaggtc gccgccttcg gaaaaggcgt cgtggccttg ccgatccacg gtgcgggcga
     1681 gggtgaacca acgacctccg ggtttgccgt tttgaggcgg cgtctcgatg atctgggtga
     1741 tgatccgacc tggtgtgcgg aaggccgaat gcagccgcca gcgcgggcag gccccgccga
     1801 aacgtgagaa gggaaagcct gccgacgcat aacgcttcga gacgtttccg gcgtgatcga
     1861 cccgcatcag gaagaatgga acacctcgcc cagtcggtcg cgacagggtg gtgagcctat
     1921 gggccgcctg ttcgaagctg acgccgaagc gcgcctgcaa ccgggcgatg tcatagcccg
     1981 tctgttccgc tgctcgctgg aatgcgccgt agggcatcag gactgcggcg acgagcgcat
     2041 tggtcagcga aaccttcagc aagtcgcggc tgatcgagtc gggcatgtcg gcggcatcga
     2101 ccatcgcagc cagttcgacg gcatgctccg ccagaaccag ctgatagatg actgcgaagg
     2161 ctcgcgaaga agggccgagg gtctcagaca gcagcagtct gc
//
LOCUS       DASVTM010000450         8381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_574368,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000450 DASVTM010000000
VERSION     DASVTM010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574368"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VF633_12355"
     CDS             <1..529
                     /locus_tag="VF633_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8471897.1"
                     /translation="VEVSDNPAANILIRELGGLDAMRNWYRRAGDDMTSVDRMEPSLN
                     VKDGEKDTTLPFQAAKNLGWILEVYQPLRFQSRYRPLWDWLIDTPTGEKRIKAGVPAG
                     WTVAHKTGTGGRGQTNDIGLVYPQAGAPIPVAVFYDAPAALAPAKRDAVVAEATRRAL
                     AALGHGAVPASGAGA"
     gene            544..2646
                     /gene="flhA"
                     /locus_tag="VF633_12360"
     CDS             544..2646
                     /gene="flhA"
                     /locus_tag="VF633_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX8471898.1"
                     /translation="MKDGLARPTGQDIKGWVMRGEVAMAVGVIGVIMLLILPVPKFLM
                     DFLLAISLVSSVLILMTALMMKRPLDFAIFPTVLLVSTLFRLGLNLASTRLILSHGHE
                     GHDAAGKVINAFGQLMMGGSFIIGVIIFAIILVVNFVVITKGSTRIAEVSARFTLDSM
                     PGKQMAIDADLSSGLITEDQAKLRRKELEQESTFFGAMDGASKFVRGDAVAGLIIVFI
                     NVIGGILIGVLQHQLPIGEAASTYVQLTIGDGLVTQVPAIIISIAAGFLVSKAGVEGS
                     ADKALVAQLATNPVSLGMVSGAAGLIGLIPGMPLIPFAALALGAGFMAWRLGRARLKP
                     QPTEAEKAAAAQAASGKPKEDVEEPIGTALTIDEVKIELGYSLLSLINDLEGRRLTDQ
                     IRALRRALAQEYGFVIPSVRILDNMRLPTQGYAIRIKEMDAGAGEVRLGSLMAMDPTG
                     RQVELPGEHTKEPAFGLPATWIDESLREEATFRGYTIVDPSTVLTTHLTEILKENMAD
                     LLSYAEVQKLMKELGEEEKKLVDELVPSVVTVTTLQRVLQALLKEKVSIRDLGAILEG
                     LAEAAPHSSSVTTLVEHVRSRLARQLCWQHKSDDGALPIVTLSPEWEQAFADALVGSG
                     EDKQLAMAPSRLQDFIRAVRDVFERAAMAGETAVLLTGPQIRPYVRSIIERFRGQTVV
                     MSQNEVHPKARLKTIGSV"
     gene            complement(2658..3380)
                     /locus_tag="VF633_12365"
     CDS             complement(2658..3380)
                     /locus_tag="VF633_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471899.1"
                     /translation="MVSWWWGVPALVAFISALFVLNGLVSLFRGRPVSGIFKIIGGGV
                     FLSLALATVLLALNVQTYARLTYERPVATIQLRQLASRYFEVTVIQPPVASGEPARTA
                     IYPVNGDDWRIEAQVLKWKPWANVLGLDTQYRLDRLSGRYEDIEQERTGARSVHALSG
                     GDVGTEVLGTTVPWKISAWDAARRYRRYVDAVDTLYGSAAYMPMADGARYEVWITQSG
                     LIARPANAAARNASAGGWTNVD"
     gene            3486..5030
                     /locus_tag="VF633_12370"
     CDS             3486..5030
                     /locus_tag="VF633_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8471900.1"
                     /translation="MTTDTTTRFPSVGRLARWREFFRAPSLARSVVFLLTGALILLLL
                     VNVSTFVMINRTAAYNVQVDATWRTRREARSLILAVQGAETAQRAFLLTGQPGFLTAF
                     EKNKAAIPRIFADFERGAEGDGVALASAEALTRITRDKFIEMSATLELARQGRIGQAV
                     SRVRTGEGQALTEELDAEMSRLDGHLGQTLERRRLSSERSALFTVLINAIAGVLILVL
                     AGIIIMLVRRYLAEITSARDELDLVNAGLEERVKARTAALIRANEEVQRFAYIVSHDL
                     RSPLVNVMGYTSELEQAGKTIDRAIVKAELEREVDAEIITAVREDMPEAIGFIRASTE
                     KMDRLINAILKLSREGRRNLTPETLDMTAMVGRIADSVHHQTEEADAEIVVEALPAFE
                     NDRMSIEQVFGNLIDNAVKYLDRSRPGHIVVSGRERPGGWVEYSITDNGRGIAPRDHE
                     RVFELFRRAGRQDTKGEGLGLAFVRNSVRRLGGEITLESELGKGSTFLLKFPKRLILD
                     EAGDTL"
     gene            5051..5452
                     /locus_tag="VF633_12375"
     CDS             5051..5452
                     /locus_tag="VF633_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8471901.1"
                     /translation="MVEDDHGHAKLIEKNIRRANISNDIVHFDSGQPALDYLFSEEVR
                     ANGPMLILLDLNLPDMQGTDILAEVKKDERLKRAPVVVLTTTDDKTEIQRCYDLGCNV
                     YITKPVDYESFAGAIRQLGLFLSVMQAPDIE"
     gene            5449..6489
                     /locus_tag="VF633_12380"
     CDS             5449..6489
                     /locus_tag="VF633_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX8471902.1"
                     /translation="MTPEKPAIRLLYIDDDAGLSRLVQKELGRHGYEVTCVGDGDAGL
                     EALAAADYDVCALDHYMPGRDGLDVLPDIMAMTSPPPVVYVTGAQEGRIAVAALRAGA
                     ADYVIKDVSEDFTSLLRSALDDALFRRRLQRENEEAVEQVRLARDRAEAMLREVNHRV
                     GNSLQLVSTFMSLQLRHLADEGARNAVREAQARIEAVAHVHRRLYTSGDMSSVAMDEY
                     LAGLIDELSKSLGPGEGSPTLALEAAPMRVSTDQAVSLGVVVTELVTNAVKYAYAPGQ
                     GGEIRIILKPGADGRALLIVEDDGPGMGDGAPKGTGLGGKIISAMASGLRSVVEIDRA
                     HKGVRAHLAFDL"
     gene            6492..6935
                     /locus_tag="VF633_12385"
     CDS             6492..6935
                     /locus_tag="VF633_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8471903.1"
                     /translation="MRPSLQFGRADPGVDYPERPTAFGLVFHEQKLACVRVERARSYY
                     DLPGGAVEAGETEEEALIREFLEETGMTVRPVERLAEAGQFFRKSDTRPMNNVGGLWI
                     AEMLSLDPGARIEIDHELVWLHPRTALAELRHDAHAWAVAKWLRT"
     gene            7030..7218
                     /locus_tag="VF633_12390"
     CDS             7030..7218
                     /locus_tag="VF633_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX8471904.1"
                     /translation="MPDQDRIDGSMKNIGGKIKEAAGNVTGDEKLKAEGKADQLGGKV
                     QNAVGGVKDSIRDADDRK"
     gene            complement(7272..7775)
                     /locus_tag="VF633_12395"
     CDS             complement(7272..7775)
                     /locus_tag="VF633_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471905.1"
                     /translation="MLTAVVAALMLQQAAGMISWNPPPPADPDGPVPAVTPATALPDW
                     ALADPFAWERSQCSPIIRKDASMEMCQARVRTDLAAALGDRLPPALAPSGIEGCRQVT
                     NPAGGYVLTCAPVRREMSASTSPVAEVCEERPTATAGGAVSFERRCTPASGPASKDGL
                     TFRLGGD"
     gene            complement(7841..>8381)
                     /locus_tag="VF633_12400"
     CDS             complement(7841..>8381)
                     /locus_tag="VF633_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8471906.1"
                     /translation="TARQGWLDIRMRPERRNTIKVLLFLDVGGSMDGHIKLCEELFSA
                     ATSEFKNLEFFYFHNCLYEGVWKDNRRRHSEQTPTWEVLNKYPGDWRAIFVGDATMSP
                     YEVTMPGGSVEHWNEEAGAVWLRRARQHWDKSVWLNPVAEKYWSYTASVKLIDEVMDQ
                     RMFPLTLDGLDKAIRALTR"
BASE COUNT         1552 a         2707 c         2664 g         1458 t
ORIGIN      
        1 ggtcgaggtc agcgacaacc cggccgccaa tatcctgatc cgcgaacttg gcgggcttga
       61 cgcgatgcgc aactggtatc gccgcgccgg cgacgacatg acctcggtcg atcgcatgga
      121 gccttcgctg aacgtcaagg acggcgaaaa ggatacgacc ttgcccttcc aggcggcgaa
      181 gaacctgggc tggattctcg aagtttatca gccgctgaga ttccagtccc gctatcgtcc
      241 gttatgggac tggctaatcg acaccccgac cggcgagaag cgaatcaagg ccggtgtgcc
      301 tgcgggctgg accgtcgccc acaagaccgg cacgggtggt cggggccaga ccaacgacat
      361 cggtctggtc tatccacagg ccggggcacc gatcccggtt gccgtcttct acgatgcgcc
      421 ggctgcgctc gctccggcga aacgtgacgc cgttgtcgct gaggccaccc ggcgcgctct
      481 ggccgcgctc ggtcacggag ccgttcctgc atccggagcc ggcgcgtgac tgacaccacc
      541 ctgatgaagg acgggctcgc ccgcccgacc ggccaggata tcaagggctg ggtgatgcgc
      601 ggcgaggtcg cgatggcggt cggcgtaatt ggcgtgatca tgctgctgat cctgccggtg
      661 ccgaaattcc tgatggattt cctgctggcg atctcgctgg tgtcgtccgt cctgatcctg
      721 atgacggcgc tgatgatgaa gcgcccgctc gacttcgcca tcttcccgac cgtgctgctg
      781 gtctcgaccc tgttccggct gggcctgaac ctcgcgtcga cccgcctgat cctcagccac
      841 ggccacgagg gtcacgacgc ggccggcaag gtcatcaacg ccttcggcca gttgatgatg
      901 ggcggcagct tcatcatcgg cgtgatcatc ttcgccatca ttctcgtcgt gaacttcgtc
      961 gtcatcacca agggttcgac ccggatcgcc gaggtctctg cccggttcac cctcgactcc
     1021 atgcccggca agcagatggc catcgacgcc gatctgtcgt cggggctgat caccgaggat
     1081 caagccaagc ttcgccgcaa ggaactggag caggaatcga ccttcttcgg ggccatggac
     1141 ggcgcgtcca aattcgtgcg cggcgacgcc gtcgcaggcc tgatcatcgt cttcatcaac
     1201 gtcatcggcg gcatcctgat cggtgtgctg cagcatcaac ttccaattgg tgaagcggcc
     1261 agcacctatg tccaactgac catcggcgac ggtctggtga cgcaggtccc ggccatcatc
     1321 atctcgatcg ccgccggttt cctcgtgtcc aaggctggcg tcgagggcag cgcagacaag
     1381 gccctggtcg cgcagctggc gaccaacccc gtcagcctcg gcatggtctc gggcgcggcg
     1441 ggcctgatcg gtttgatccc cggaatgccg ctgatcccgt tcgcggcgct ggctctgggc
     1501 gcgggcttca tggcctggcg tctgggtcgt gcgaggctca agccccagcc gaccgaagcc
     1561 gagaaggcgg ccgccgctca ggcggcctcc ggcaagccca aggaagacgt cgaggagccg
     1621 atcggcacgg cgctgaccat agacgaagtc aagatcgagc tcggctattc gcttctgtcg
     1681 ctgatcaacg acctcgaagg tcgtcgcctg acggaccaga tccgcgctct ccgtcgcgcg
     1741 cttgcgcagg agtacggctt cgtcatccca tcggtgcgca ttctcgacaa catgcgcttg
     1801 ccgacccagg gctatgcgat ccgcatcaag gagatggatg ccggggcagg ggaggtacgt
     1861 ctgggctcgc tgatggccat ggaccccacg gggcgccagg tcgaactgcc cggcgagcac
     1921 accaaggagc ccgccttcgg tctgcccgcc acctggatcg acgagagtct tcgcgaagag
     1981 gcgacgttcc ggggctatac gatcgttgat ccctcgacgg tgctgacgac gcatctgacc
     2041 gagatcctca aggagaacat ggccgacctg ttgtcgtacg ccgaagtcca gaagctgatg
     2101 aaggagctgg gcgaggagga gaagaagctg gtcgacgaac tggtgccttc ggtcgtcacc
     2161 gttacgacgc tacagcgcgt gcttcaggcc ctgctcaagg agaaggtctc gatccgcgat
     2221 ctgggcgcca ttctcgaggg gctggccgaa gccgcaccgc attcatcgtc ggtcacgacg
     2281 ctggtcgagc acgtccgcag ccgtctggcc cgtcaactgt gctggcagca caagagcgac
     2341 gacggcgccc tgccgatcgt caccctgtcc cccgaatggg aacaggcctt cgccgacgct
     2401 ctggttggct cgggcgagga caagcaactc gccatggccc cgtcgcgcct gcaggacttc
     2461 atccgcgccg tccgagacgt gttcgaacgc gccgccatgg cgggtgagac cgccgtcctg
     2521 ctgaccggcc cgcagatccg cccctacgtc cgttcgatca tcgaacgttt ccggggacag
     2581 accgtggtga tgagccagaa cgaagtgcat ccgaaagccc gcttgaagac gatcggctcg
     2641 gtctaggaga ccccagccta gtcgacgttc gtccacccgc ctgccgaggc gttgcgggcg
     2701 gccgcattgg cggggcgggc gatcaggcca gactgggtga tccagacctc atagcgggcg
     2761 ccgtcggcca tcggcatata ggcggcgctg ccgtagaggg tgtctaccgc atcgacatag
     2821 cggcggtagc gccgtgcggc gtcccaggcg ctgatcttcc acggcacggt cgtgcccaga
     2881 acctcagtcc cgacatcgcc gccggacagg gcgtggacgc tgcgggcgcc ggtgcgttcc
     2941 tgttcgatgt cctcgtagcg accggacaag cgatccagcc gatactgcgt atccagcccc
     3001 agcacattcg cccacggctt ccatttcagc acctgagcct caatgcgcca gtcgtcgccg
     3061 ttgacgggat agatggcggt tcgcgcgggc tctccagacg cgaccggcgg ctggatgacg
     3121 gtcacttcga aataccgtga cgccagttgt cgcaactgaa tcgtggccac gggccgctca
     3181 taggtcagcc gggcataggt ctggacattc agggccagca acacagtggc aagagccagg
     3241 ctgaggaaga cgccgccgcc gatgatcttg aagattccgc tgacaggtcg cccccggaac
     3301 agagacacga gcccgttcag aacgaacagc gcgcttatga aggccacaag ggccggtacg
     3361 ccccaccacc acgaaaccat ctcgccgacg ctcccctgcg acgtgagccg tccatatggt
     3421 cgccggcgaa ctgtggtcgc ccaacgacgc tggacagcag cccaagccaa gctaaacctt
     3481 gcgccatgac gacagacacc accacccggt tcccctcagt ggggcgtttg gccagatggc
     3541 gggagttctt tcgggccccg tcgctggccc gctcggtggt cttcctgctg actggagcgc
     3601 tcattttgct gctactggtc aacgtcagca cctttgtgat gatcaatcgt acggccgcct
     3661 acaacgtaca ggtcgacgcg acctggcgga cccgtcgtga agcccgttcg ctgatattgg
     3721 cggtacaggg agcggagacg gcccagcgcg cctttctgct caccggccag ccaggcttcc
     3781 tgacggcgtt cgaaaaaaac aaagccgcca tccctcgcat tttcgccgat ttcgagcggg
     3841 gggccgaggg cgatggggtt gcattggcgt cagctgaagc cctgacccgg atcacccgcg
     3901 acaagttcat cgagatgtcg gctacgctcg aactcgcgcg tcagggccgc atcggtcagg
     3961 cggtaagccg ggtgcggacg ggggagggtc aagccctgac cgaagaactc gatgcggaaa
     4021 tgagccggct cgacggccat ctcgggcaaa ccctcgagag acgccgctta agctccgaac
     4081 ggtctgcgct tttcaccgta ctgatcaacg cgatcgcagg tgtgctgatt ctggtgctcg
     4141 cggggatcat catcatgctg gtgcggcgat atctggcgga aatcacctcc gcacgggatg
     4201 aactggatct cgtgaacgcc ggtctggagg agagggtcaa ggcccgaaca gcggccctga
     4261 tccgcgccaa tgaagaggtc cagcgcttcg cctacatcgt cagccatgac ctgcgttcgc
     4321 cgctggtcaa cgtcatgggc tacacctccg agctggaaca ggcgggcaag acgatcgacc
     4381 gcgccatcgt gaaggccgaa ctcgaacgtg aggtcgatgc cgaaatcatc accgccgtac
     4441 gcgaggacat gcctgaggcg atcggcttca tccgcgcctc gaccgagaaa atggacaggc
     4501 tgatcaacgc catcctgaaa ctatctcgcg aggggcggcg caacctgacg cccgagaccc
     4561 tcgacatgac cgccatggtc ggccgtatcg ccgacagcgt ccatcaccag accgaagaag
     4621 cggatgccga gatcgtggtc gaggctctgc cggcattcga gaacgaccga atgtcgatcg
     4681 aacaggtgtt cggcaacctg atcgacaacg ccgttaaata tctcgatcgc agccgcccgg
     4741 gccatatcgt ggtttcgggg cgcgagcgac ctggtggctg ggtcgaatat tccatcactg
     4801 acaacggccg cggcatcgcc cctcgcgatc acgaacgagt cttcgaattg ttccgtcgcg
     4861 cgggacgcca ggacaccaag ggcgaagggc tcggcctggc cttcgtgcgc aacagcgttc
     4921 gccgactggg cggcgaaatc acgctagagt ccgaacttgg taagggctcg acttttcttc
     4981 tcaaattccc caaacgcctc atcctcgacg aggccggaga taccctgtga gcacccctgt
     5041 taagatcgtc atggtcgaag acgaccatgg tcacgccaag ctgatcgaaa agaacattcg
     5101 gcgagccaac atctccaacg acatcgtgca tttcgattcc ggtcagccgg cgctggacta
     5161 cctcttctcg gaagaagtgc gcgccaacgg cccgatgctg atcctgctgg acctgaacct
     5221 gcccgacatg cagggcaccg acatcctggc cgaagtgaaa aaggacgagc gcctcaagcg
     5281 cgcacccgtc gtcgtcctga cgaccaccga cgacaagacc gagattcagc gctgttatga
     5341 tctgggctgc aacgtctaca tcaccaagcc agtcgactat gaatcgttcg ccggcgccat
     5401 ccgccagctc ggcttgttcc tgtccgtcat gcaggcgccc gacatcgaat gacccctgaa
     5461 aagccggcca ttcggctcct gtacatcgac gatgacgccg gcctgtcccg actggtccag
     5521 aaggaactgg gccggcacgg gtatgaggtc acgtgcgtcg gcgacgggga tgcggggctc
     5581 gaggcgctgg cggcggcgga ttacgatgtc tgtgcgctgg accactatat gccgggccgc
     5641 gacggcctcg atgtgctccc cgacatcatg gcgatgacca gcccgccccc ggtcgtctat
     5701 gtcaccgggg cgcaagaagg ccggatcgcc gtcgcagccc tgcgcgcggg cgctgctgac
     5761 tatgtcatca aggatgtcag cgaggacttc acctcgctgc tgcgttccgc actggatgac
     5821 gccctgttcc gccgtcgctt gcagcgtgag aatgaagagg ccgtggaaca ggtccggctc
     5881 gcccgcgacc gggccgaggc catgctgcgt gaggtcaacc accgggtcgg caactccctg
     5941 caactggtct cgaccttcat gtccctccag cttcgtcacc ttgctgacga aggcgcccgc
     6001 aacgccgtca gggaagcgca agccaggatc gaagccgtcg cgcacgtgca ccgtcgcctc
     6061 tacacctcgg gcgacatgtc cagcgtcgcc atggacgaat atctggcggg gctgatcgat
     6121 gaactcagca agtcgctcgg gccgggcgaa ggatccccga cactggcgct cgaggcagcg
     6181 cccatgcggg tgtcgacgga tcaggccgtg tctctcggcg ttgtggtcac cgaactggtc
     6241 accaacgccg tgaaatacgc ctatgcaccg gggcagggcg gcgagatccg catcatcctc
     6301 aagcccgggg ccgacggccg ggccttgttg atcgtcgagg atgatgggcc ggggatgggc
     6361 gacggggctc ccaagggcac cggccttggc ggcaagatca tttcggccat ggcctctggc
     6421 ctgcgttcag tcgtcgagat cgatcgtgct cacaagggcg tgcgcgccca ccttgctttc
     6481 gatctttgat catgaggccg tcactacagt tcggccgcgc cgatcccggc gtcgactacc
     6541 ccgaacgtcc gacagccttt ggtctggtgt tccatgagca gaagctggcc tgtgtccgcg
     6601 tcgagcgcgc ccggtcctat tacgacctgc cgggcggagc ggtggaggca ggcgagacgg
     6661 aggaagaggc cctgatccgc gagtttctcg aggagaccgg gatgaccgtc cgtccagtcg
     6721 aacgattggc agaggcggga cagtttttcc gcaagtccga cacccggccg atgaacaacg
     6781 tgggcggttt gtggatcgcc gagatgcttt ccctggatcc cggcgcccgg atcgagatcg
     6841 accacgaact cgtctggctg catcctcgca ccgctttggc cgagctacgt cacgacgctc
     6901 atgcgtgggc cgtggcgaag tggctcagga cctgacagca gttcgaaagg cgggaactcg
     6961 actctctccg gcgggttcaa tgaaccgaag cgacgccgac ccggcggcgt tgaaaacaag
     7021 gagtttccca tgccagatca agatcgcatc gacggctcga tgaagaacat cggcggcaag
     7081 atcaaggaag ccgccggcaa cgtcaccggc gacgaaaaac tgaaagccga aggcaaggcc
     7141 gatcagctcg gcggcaaggt ccagaacgcc gtcgggggcg tcaaggactc gatccgggac
     7201 gcagacgacc gcaagtaaga acgcttaaag agatcgaaag cgccgtccgt tcgcgggcgg
     7261 cgtttttgcg ttcagtcccc gccgaggcgg aaggtcagac cgtcctttga tgccggtccg
     7321 ctcgcgggtg tgcagcggcg ctcgaagctg acggctcctc cagccgtcgc tgtcgggcgt
     7381 tcttcacaga cctcggcgac cggggacgta ctggctgaca tttcgcggcg gactggggcg
     7441 caggtcagga cgtagccacc ggccgggtta gtcacctgtc ggcacccctc gatgccgctc
     7501 ggggccagag cgggcggcag gcgatcgccg agcgctgcag ccagatctgt ccgaacccgg
     7561 gcctggcaca tctccatgct ggcgtccttg cggataatcg gactgcactg cgaccgctcc
     7621 caggcgaagg ggtcggccag agcccagtcg ggcagcgcgg tagcgggcgt cactgccggg
     7681 accgggccgt cggggtctgc cggcgggggt gggttccacg agatcatccc ggcggcctgc
     7741 tggagcatca gggcggcgac gacggcggta agcatcttgg atactcctga acgatccggt
     7801 cagagtgcgc ccgccggtcg cgaaaatcca ccggatgatt ctagcgagtg agagcccgta
     7861 tggccttatc gaggccgtcc agcgtcagcg ggaacatccg ctggtccatg acttcgtcga
     7921 tcagtttcac cgaagccgta tagctccagt atttttcggc caccgggttg agccagaccg
     7981 acttgtccca gtgctggcgg gcacgccgaa gccagaccgc gccggcttcc tcgttccagt
     8041 gttcgaccga accgccgggc atggtcacct cataggggct catggtcgca tcgccgacga
     8101 agatcgcccg ccagtctccc gggtatttgt tgagcacttc ccacgtcggg gtctgctcgg
     8161 aatgccggcg tcgattgtcc ttccacacac cctcgtagag gcagttgtgg aagtagaaga
     8221 attcgaggtt cttgaactcg gacgtggcgg cggagaacag ctcctcgcag agtttgatat
     8281 gcccatccat cgacccgccg acgtcgagga acagcaacac cttgatggta ttacgccgct
     8341 ccggccgcat gcggatgtcg agccagccct gacgcgcggt c
//
LOCUS       DASVTM010000451         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_892999,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000451 DASVTM010000000
VERSION     DASVTM010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892999"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VF633_12405"
     CDS             complement(<1..991)
                     /locus_tag="VF633_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX8471907.1"
                     /translation="MAHDLRQAFRRPGLPGYGVAIGAWAIAFAIRFLLSHWFPPGFPY
                     LTFFPAVVVVAYFAGLRPAVLTAVLSGLTAWWFWIGPAGFDVSSATAVALIFYVAVVA
                     VDIFFIVGMDSATRKLALEVERNAALARGRDLLLKEVQHRVSNNIQVVSALLRMEASA
                     TADPGARRALTEASARTAMIARIQHGLSDVDGRATPFEAVARPMVDDALAASGRNDVA
                     IEIEPSLIALSAEEATPVMLTMLECVNNALEHGVANRAGRITITLREEDGERRLEVHD
                     DGAGLSEGFDLSTTKSLGLRIISGLAKQLGGVFTLENAHPGALSRLTYQSRATE"
     gene            complement(1147..>1905)
                     /locus_tag="VF633_12410"
     CDS             complement(1147..>1905)
                     /locus_tag="VF633_12410"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8471908.1"
                     /translation="PDGGDIWDLSHRSQVVDEVGRKVLDDCDRDPACRERTGGSAADG
                     LRRIVDDPERAKLFPGGKPKAFLGSLLDSPDARALIPDIIADVLAGRSAAIDRAAARM
                     EAFAAPFAAPEAASSIPLVALISASENNARLDLTGKDIAAEAEVLLFTSSLPAQLSGG
                     GDITYPRDVAFGADPARLPAVLVLQGDMDPKTPHAGAVVHADQLAKVGKVRFVTVEGA
                     PHYIMLTDPDRAESAIASFLSENSPARTGYATAH"
BASE COUNT          357 a          618 c          617 g          313 t
ORIGIN      
        1 cctctgtcgc gcggctctgg taagtgaggc gggacaaggc tccgggatgc gcattttcga
       61 gggtgaagac accgcctagc tgtttggcca ggccggaaat aatgcgaagg cccaggcttt
      121 tggtcgtcga caggtcgaag ccttccgaaa ggccggcgcc gtcgtcatgg acctccagtc
      181 gccgctcgcc gtcttcttcg cgcaaggtga tcgtgatgcg tcctgcacgg ttcgccactc
      241 catgttccag cgcattgttg acgcattcca gcatggtgag cattaccggc gtggcctcct
      301 cggcgctcaa ggcgatgagg gatggctcaa tctcgatggc gacatcgttg cgcccgcttg
      361 ccgccagggc atcgtcgacc atggggcggg ccacggcctc gaacggcgtc gcgcgtccgt
      421 cgacatctga caagccatgc tggatccgcg cgatcatggc cgtgcgcgcc gaagcttctg
      481 tcagggcgcg ccgcgcacca gggtccgccg tagccgaggc ttccatccga agcagggcgc
      541 tgacgacctg gatgttgttc gacacccggt gctggacttc cttgagcagc agatcacgac
      601 cgcgggcgag ggcggcgtta cgctccactt cgagagccag tttgcgggtc gcggagtcca
      661 tgccgacaat aaagaagata tcgaccgcta ccacagccac ataaaaaatc agggcgacgg
      721 ccgtggccga gcttacgtca aagcctgcgg ggcctatcca gaaccaccac gccgtcagac
      781 ccgacagcac tgcggtcagc acggccggac gcaatccggc gaaataggcg acgaccacga
      841 ctgcgggaaa gaacgtcaga tacggaaagc ccggcggaaa ccagtgcgac agcaggaagc
      901 ggatcgcaaa cgcgatcgcc cacgcgccga tggcgacgcc gtaaccgggc aggccgggcc
      961 ggcgaaacgc ctgtcgaaga tcatgtgcca atgatgaata cccgcacgcc cgaaagggcg
     1021 cagctcatcc tgtaccctta gccaagctat tcacggccgc caagacagcg ggctccacga
     1081 tgggcaggcg atttcagggg ccgtgccgtt cgcaccccga tggctgtcgg cgcggcggca
     1141 tgacgctcag tgcgccgtcg cgtatccggt ccgtgccggt gagttctcgg acaggaagct
     1201 cgcgatggcg ctctccgccc gatccgggtc ggtcagcatt atgtagtgag gcgcgccctc
     1261 gacggtcacg aaccggacct tcccgacctt ggccagttgg tcagcgtgga cgacggcccc
     1321 cgcgtgcggc gtcttgggat ccatgtcgcc ctgaagaacc agaaccgccg gcaaacgcgc
     1381 cggatcggcg ccaaacgcga cgtctctggg gtaagttata tcgccaccgc ccgacaactg
     1441 tgccggcagg ctgctcgtaa agagcaatac ctcggcttcg gcggcaatat ccttaccagt
     1501 caggtcgagg cgggcattgt tctcagaggc ggagatcagg gcgaccaagg gaatggagga
     1561 ggccgcttcc ggcgccgcga agggtgcggc gaacgcttcc atgcgggccg cggcccggtc
     1621 tatcgcggcg ctccgtcccg cgaggacgtc tgcgatgata tccgggatca gcgcgcgcgc
     1681 atcggggctg tcgagcaagg atccgaggaa ggccttgggt ttgccgcccg ggaacaattt
     1741 tgcgcgttca gggtcatcca cgatccgtcg aagcccgtcg gcagccgagc cgcctgttcg
     1801 ttcacgacag gcggggtccc ggtcacagtc gtccagcacc tttcggccca cctcatccac
     1861 gacctgggac cgatggctca gatcccagat atcccctccg tcagg
//
LOCUS       DASVTM010000452         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_98736, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000452 DASVTM010000000
VERSION     DASVTM010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98736"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VF633_12415"
     CDS             complement(<1..307)
                     /locus_tag="VF633_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8471909.1"
                     /translation="MFIVPKTAGGPPRLLIGILAALVLALLVVGGVLLARGHGKPDAV
                     HQTVAQLPLRPPVVVVENLPKLKAALDAQPRFMGAVLVARGDNVLFRQTYGPANVEKG
                     "
     gene            complement(341..1300)
                     /gene="era"
                     /locus_tag="VF633_12420"
     CDS             complement(341..1300)
                     /gene="era"
                     /locus_tag="VF633_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX8471910.1"
                     /translation="MTDTEIPNPNAKAGFAAIIGAPNAGKSTLVNRLTGSKVSIVTQK
                     VQTTRFPVRGIAITGDAQIVLVDTPGIFTPRRRLDRAMVASAWGGASDADVVVHLIDA
                     ASHIASQGKEGTGADRKSAEDTETIIANLTASGTKVILALNKIDGMRRDTLLALSHSL
                     FETGVYSEVYMISAATGDGVEDLKTRLGMSMPAGHWLYPEDQSADVPIRVLAAEITRE
                     KLYLRVHEELPYSAAVETTAFDEKADGSARIEQTIYVERESQRPIILGKGGQTLKWIG
                     QKSREELTVLLDRPVHLFVTVKVDPKWQDSRALYAQFGLDYDV"
     gene            complement(1297..2010)
                     /gene="rnc"
                     /locus_tag="VF633_12425"
     CDS             complement(1297..2010)
                     /gene="rnc"
                     /locus_tag="VF633_12425"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX8471911.1"
                     /translation="MANSRTEAVAELVRRLGHEFRDPRLLDRALTHASVGEGVAPQTS
                     KAPRDNERMEFLGDRILGLLVADRLHTDFPEADEGQLSSRLHTLVDKHACGRVGAALG
                     IGDAVRLSPGETKSGGRRKEGVIADAVEAVLAAVYLDGGLDAARKVFESAWAEELARP
                     SERSLNNPKSVLQEWAQAQGLPLPTYTITGRTGSDHAPTFTIQVLVDGIEPLTAPGRS
                     RQDAEKAAATAMLQREGVI"
     gene            complement(2010..2948)
                     /gene="lepB"
                     /locus_tag="VF633_12430"
     CDS             complement(2010..2948)
                     /gene="lepB"
                     /locus_tag="VF633_12430"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8471912.1"
                     /translation="MSDEHKPHQPEATSGEPVEAHPHAATSADKRPVWSDQGDAPFDD
                     DVTSGKTEKTPKAARVEEVEERSAGSEGLEIVKTVVFALLIAFVLRVLLFQPFTIPSA
                     SMEPNLYEGDYIVVSKWSYGYSKYSSGLPINLPIGDGRVFGRMPNRGDIVVFKLPRDN
                     KTDYIKRVIGLPGDKIQMIANKLYINGAPVQDAVVSETEVADAWGARAATEARETLPN
                     GKSFMTQDFGPGNDLDDTPVYEVPTGYYFMMGDNRDNSIDSRVEQSAGVGFVPAENLV
                     GKAQIIMFSWSPGASLWNPISWFSKIRPSRFFNLLH"
     gene            complement(3014..>3262)
                     /locus_tag="VF633_12435"
     CDS             complement(3014..>3262)
                     /locus_tag="VF633_12435"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX8471913.1"
                     /translation="RFAAKEACAKALGTGMRRDVYWKDMGVVNKRSGQPTLALTGHAA
                     EHLARLTPPGHAPHIHLTLSDEHPYALAFVVIEALPLS"
BASE COUNT          534 a         1014 c         1121 g          593 t
ORIGIN      
        1 ggcccttctc cacattggcc ggaccgtagg tctggcggaa cagcacgttg tcgccccggg
       61 ccaccagaac cgcgcccatg aaccgaggct gagcgtcgag cgcggccttc agcttcggca
      121 ggttctcgac caccacgacg ggcgggcgca gcggcagttg ggcgaccgtc tggtggacgg
      181 cgtcaggctt gccgtggcct ctcgccagca agacgccgcc gaccaccaga agggccagaa
      241 ccagagcggc gaggatgccg atcagcagcc ggggcggacc tccggcggtc ttgggaacga
      301 tgaacaacga aacgctaacc tactgtagcc ggacgtcgga tcagacgtcg taatccagac
      361 cgaactgggc gtagagggcc cggctatcct gccatttggg atcgactttg accgtgacga
      421 aaagatggac agggcggtcc agaaggacgg tcagttcctc acgcgacttc tggccgatcc
      481 atttaagggt ctggccgccc ttgccgagaa tgatcgggcg ctggctctcg cgctcgacat
      541 agatggtctg ttcgatgcgg gccgaaccgt cggccttctc gtcgaaagcc gtggtttcga
      601 cggctgccga atagggcagt tcctcgtgga cgcgcaggta gagcttctcg cgcgtgatct
      661 cggcggccag aacgcggatc ggcacatcag cgctctgatc ctcgggatag agccagtgac
      721 cggccggcat ggacatgccc agacgggtct tcaggtcctc gacgccgtcg ccggtcgctg
      781 ccgagatcat atagacctcg gaatagacgc cggtctcgaa caggctgtgc gacagggcca
      841 acagggtgtc gcggcgcatg ccgtcgatct tgttcagcgc cagaatgacc ttcgtgccgc
      901 tggccgtcag gttggcgatg atggtctcgg tgtcctcggc ggacttgcgg tcggcgcccg
      961 tgccttcctt gccctgcgag gcgatgtgcg aggctgcgtc gatcagatgg acgacgacgt
     1021 cggcgtccga cgctccgccc caggccgagg cgaccatggc gcggtccaga cggcggcgcg
     1081 gggtgaagat gccgggggtg tcgaccagaa cgatctgggc gtcgccggtg atggcgatgc
     1141 cgcgcacggg gaagcgcgtc gtctgcacct tctgggtgac gatcgaaacc ttggaccctg
     1201 tcagccggtt gaccagcgtc gacttgccgg cattgggcgc gccgatgatg gcggcgaagc
     1261 cggctttggc gttcgggttc gggatctcag tatcggtcaa atgacgcctt ctcgttgcaa
     1321 catggccgtc gcggcggcct tctccgcatc ctgacgcgaa cgtccgggag cggtcagggg
     1381 ttcgatgccg tcgaccaaca cctggatggt gaaggtcggg gcgtggtccg agccggtgcg
     1441 gccggtgatg gtgtaggtgg gcagaggaag accctgcgcc tgggcccatt cctgcagaac
     1501 ggacttggga ttgttcagtg agcgttcgga cggacgggcc agttcctcag cccaggcgct
     1561 ctcgaacacc ttgcgcgcag cgtcgaggcc gccgtcgaga tagaccgccg ccagcaccgc
     1621 ttcgacggcg tcggcgatga cgccttcctt gcgacggccg cccgacttgg tctcgcccgg
     1681 cgacaggcgc acggcgtcgc cgatgccgag agccgcaccg acgcgaccgc aggcgtgctt
     1741 gtcgaccagc gtatgcaggc gcgaggacag ttggccttcg tcggcttcgg ggaagtcggt
     1801 gtgcaggcgg tcggcgacca gcaggccgag gatccggtcg cccagaaact ccatccgctc
     1861 gttgtcgcgt ggggctttgg acgtctgcgg ggcgacgccc tcgccgacgc tggcgtgggt
     1921 cagggcgcgg tcaagcagac gcgggtccct gaactcgtga ccgagccggc ggaccagttc
     1981 ggccaccgcc tctgtccttg aattcgccat cagtgaagca agttgaagaa gcggctggga
     2041 cggatcttcg agaaccagct gatcgggttc cacaggcttg cgcccggcga ccacgagaac
     2101 atgatgatct gcgccttgcc gacgaggttc tcggccggga cgaagcccac gcctgcggac
     2161 tgctcgacgc ggctgtcgat cgagttgtcg cggttgtcgc ccatcatgaa gtagtagcct
     2221 gtcggcactt cgtagaccgg ggtgtcgtcg agatcgttac cgggaccgaa gtcctgggtc
     2281 atgaagctct tgccgttggg cagggtttca cgggcctcgg tggcggcgcg agcgccccag
     2341 gcgtcggcga cctcggtctc gctgacaacg gcgtcctgaa ccggtgcgcc gttgatgtag
     2401 agcttgttgg cgatcatctg gatcttgtcg ccgggcaggc cgatcacgcg cttgatgtag
     2461 tcagtcttgt tgtcacgcgg cagcttgaac acgacgatat cgccacggtt cggcatgcga
     2521 ccgaacacgc gaccatcgcc gatcggcagg ttgatcggca ggccgctgga atacttggaa
     2581 tagccgtacg accatttcga gacgacgatg tagtcgcctt catacaggtt gggctccatc
     2641 gaggccgacg ggatggtgaa gggctggaac agcaggaccc gcagcacgaa ggcgatcagc
     2701 agggcgaaca cgaccgtctt gacgatctcg aggccttcgc tgccggccga gcgttcctcg
     2761 acctcttcga cgcgagccgc cttgggggtt ttttccgtct tgccggaggt cacgtcgtca
     2821 tcgaacggcg catcaccctg gtcgctccag accgggcgct tgtcggcgct ggtcgccgcg
     2881 tgcggatggg cctctaccgg ctcgccagac gtcgcttccg gctggtgcgg tttgtgttcg
     2941 tcgctcatcg ttgagaaggc ctgcgtcccc gaccgctttg cgcggttgtc taaggggcgc
     3001 acctatagcc tgattacgac agcggcaagg cctcgatcac cacgaaggcg agtgcgtacg
     3061 ggtgttcgtc actcagcgtc agatgaatgt gcggcgcatg gccgggcggc gtcagtcggg
     3121 ccagatgctc ggcggcgtgg ccagtcagcg ccaacgtcgg ctgaccacta cgcttgttca
     3181 ctacacccat gtccttccag tagacgtccc gccgcatgcc agtgccgagc gccttggcgc
     3241 aggcctcctt ggccgcgaac cg
//
LOCUS       DASVTM010000453        16445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_948875,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000453 DASVTM010000000
VERSION     DASVTM010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 16445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16445
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948875"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VF633_12440"
     CDS             complement(<1..568)
                     /locus_tag="VF633_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX8471914.1"
                     /translation="MSDTGFAEPENDEFPSAASERVLNQDEIDSLLGFDLGDDDGSER
                     TGIRAIINSALVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDNISSIRFGDY
                     LNSIPLPAILGVFRAEELDNYGLLTVDSNLIYSIVDVLLGGRRGTAALRIEGRPYTTI
                     ERVLVQRMVEVILADARAAFEPLTPIHFN"
     gene            complement(568..1218)
                     /locus_tag="VF633_12445"
     CDS             complement(568..1218)
                     /locus_tag="VF633_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX8471915.1"
                     /translation="MLKLGKKKGGAKDAATDLAVAHGLEVDGEAPPKKKLPMLLIIIG
                     AVVLLVLVGGGVAFFLMQPKPAAAEGEHGEEKAAEHGKKEEKKGGHGEKKEGEKGAEG
                     EDATAGVITEGPDGVIFYTLPDMVVNIQSPDGKPTFLQLKLTLEMHDAELAATLQEEA
                     PRMQDMFQGFLRELRPEDLAGSAGSYQLRTEILRRVNLIAAPGKVDAVLIEKMLVQ"
     gene            1401..2138
                     /gene="flgF"
                     /locus_tag="VF633_12450"
     CDS             1401..2138
                     /gene="flgF"
                     /locus_tag="VF633_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX8471916.1"
                     /translation="MENAAYIGLSRQMTLRRELDIVANNIANANTTGFKVEQLLVGTE
                     VGARARNDSIKPGASFVLDNGVGRDFGQGALQQTGRSLDFGIEGEGAFFTVNDGTTDA
                     YTRDGAFTLDPTGKLVTKAGLAVQGDGGDIILDPALGAAVIGEDGTISQNGALVGKLT
                     LARFETLGALSKEGDGLYRNRSNSTPIDATGAKISQGSLEASNVNPLIEITSMIEISR
                     AYERATRMIENLNDLSKRAVERLGRPN"
     gene            2151..2939
                     /gene="flgG"
                     /locus_tag="VF633_12455"
     CDS             2151..2939
                     /gene="flgG"
                     /locus_tag="VF633_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX8471917.1"
                     /translation="MRALRTAASGMAAQQLNVEVISNNIANMNTIGYKRQRAEFQDLM
                     YQNVERMGAQSSSAGTVVPTGIQVGLGVKAGSVYRITEQGTPSNTGNTYDVAVDGKGY
                     FQITLPSGEIGYTRAGNFSLSPEGNLVTEDGYAVEPAITIPQNTTDVSISKTGQVSAI
                     IDGQTDPQIVGQLELANFFNEAGLEAVGDNLLMETAASGPATVAAPGEPGFGQLLQGY
                     TESSNVDAVSEISSLIIAQRAYEMNSKVISTADDMMSVTAQVKS"
     gene            2943..3770
                     /gene="flgA"
                     /locus_tag="VF633_12460"
     CDS             2943..3770
                     /gene="flgA"
                     /locus_tag="VF633_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEX8471918.1"
                     /translation="MRIAIAFCLALIAAPALAGPVTLRPQPVDEDGRVTFGDIFDGAG
                     SAANVVIGTRTGPSVVFEAGQLQGLALRSGLQWANPRGLSRVVVREGSASPAAPAAVL
                     TPVSTVAAPSRPATTRANPTVSSASAARPGATSDVLTYARNLSTGDVIQPEDVIWTTV
                     QAHMAPGGSPQDADQVIGLSARRALRAGAPVSSRDLASPQVIARNDLVEVAYLVGGVK
                     LTITGRATRNASIGEAVSVLNTTSNRTIDAVATGPGKAVVGPAAQSARSNPQQFAAR"
     gene            3784..4545
                     /gene="flgH"
                     /locus_tag="VF633_12465"
     CDS             3784..4545
                     /gene="flgH"
                     /locus_tag="VF633_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX8471919.1"
                     /translation="MRKLLILSIATAALSLGACSTAVEAIRGPELAPIGYPAALVPTS
                     QAYLPSPEEQAATRARQNGAMAASANSLWRTGARTFFGDQRARRVGDILTVSISIDDK
                     AQTSNSTTRARTNDASGGVTNFFGLENSLGRAFPGGFDPSNMVGLSGQSNATGSGAVS
                     RSEKVDLTIAAVVTDVLSNGNLVIQGRQEVRTNREVRELTVAGIVRPEDISSGNTINH
                     TQIAEARISYGGRGDISRVQATPAAQSLVERFSPF"
     gene            complement(4638..5471)
                     /locus_tag="VF633_12470"
     CDS             complement(4638..5471)
                     /locus_tag="VF633_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471920.1"
                     /translation="MSFSATEAAFEGFRVTRHHPVAVLAWALVALISLFAMYLMVAPI
                     LSPIAGEFQAVMASGGKLQPSLALQTQLSYAALATVPVSMVAQAILMPAVYRAMTNTG
                     RDRFGFLRLGREELRTLGALAIVTIVSLIVNQAGESLAALALASGIGAVALLIQVAAM
                     IAGIYLSVRLVFVVPAAFAEGRIDLKAGWQATAGLFWSLLGMAIIAGFMACIVMLLLG
                     IVALPISVGVVSGGGATPASLVALGGLMLLMALAVALSMTVLAAPFMTAYRAAKGLTH
                     I"
     gene            complement(5505..6464)
                     /locus_tag="VF633_12475"
     CDS             complement(5505..6464)
                     /locus_tag="VF633_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471921.1"
                     /translation="MTGLTMTARFAIGEALQTPFRLLRHQPLALFAWGAMRVVLTIAA
                     FSLLLPMLVALPFGQATTPEEMEQVIANFLPIQGGLGLMNLVGLLLSVVIWTAAMRAA
                     MAPVGTKDRFLFLRLGFDEVYVGVVMLVIVVGLYFGTFVLTLIGAVLCAILWQVSELT
                     AVLVTIAYGLAVFAAALWAAGRATLFIPASLALHTIAFEQGWKLGKGQGWRLAATGVL
                     AWLILMAVTLVIGALVITVFFIAALMLHVSLPNPNDIHSFADLTEFGRPFLVPALVLA
                     LPLALFDGFSHAMFGAPVASACKQLMDAAEIPNEGDADPKPAL"
     gene            complement(6461..7288)
                     /locus_tag="VF633_12480"
     CDS             complement(6461..7288)
                     /locus_tag="VF633_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471922.1"
                     /translation="MAESPLNSSLLNKAGRLDLAEALAEAVGRMGEMARGAGPALALT
                     TALALGVVLLRLSGAVEAVLLVLTLIAGLAAFGATTRIGTAIDLETARRSGLRRGGLQ
                     LGKPEARLAGATLLCALFMTIMLALLMLVALALFGGAGLNAEAVEARDWAAVGPTWKL
                     MLLAGVAVVVLGAPVLFAVRLSLYAPATVGRGQMISLTATGLTNRAILPLLAGLIACA
                     VPGLIWLGLVVAGVLAGKAAGVVAILMLVAIQWPLTSAFLGAAYRRLETHQLLGVWQ"
     gene            7386..8780
                     /gene="trmFO"
                     /locus_tag="VF633_12485"
     CDS             7386..8780
                     /gene="trmFO"
                     /locus_tag="VF633_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX8471923.1"
                     /translation="MTSTSDIKPIHVIGGGLAGSEAAWQIANAGVPVILHEMRGVPGV
                     KTDAHHTDGLAELVCSNSFRSDDWEHNAVGLLHAEMRELGSIIMASAHDHQVPAGGAL
                     AVDRDGFSQSVTAKLEAHPLITICREEIAGLPPEDWDNVIVATGPLTSPALAEAILKL
                     TGEESLSFFDAIAPIVHAESIDFDIAWRQSRYDKEGPGGDAAAYVNCPMNKAEYEAFI
                     DALIDGPKAEFKEWENVPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPRDPLVKAY
                     AIVQLRQDNALGTLFNIVGFQTKLKHGAQAETFRMIPGLQNAQFARLGGLHRNTYLNS
                     PHLLDRQLRMKSMPRLRFAGQVTGVEGYVESAATGLLAGRLAAAERLGQPLEAPEAHT
                     AIGALVEHITGGHLTGSKFQPMNINYGLLPPLETPKVDEAGVKIPLKERGRAKKRLMS
                     IRAIESLKAWRDAA"
     gene            8822..9466
                     /locus_tag="VF633_12490"
     CDS             8822..9466
                     /locus_tag="VF633_12490"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HEX8471924.1"
                     /translation="MIAIVRAFLVLVVVAMAASANARDKSPYPPQPTLMDRQDLRRTH
                     DGRPVYVLNTDFHYCSREYGLVVTVPSTFVTDLISIPKLARWIKTADSPGYQAAIVHD
                     WLYASGNGSEAERRQADYIFYEALVDYDVDPMVRLAMHQMVRKFGKDGYGLKGDWLFA
                     DVRLHKAAPEALGRTVAPAMTPDASCSAFQNAVESGEDYLARIRPATVGGSRPQ"
     gene            9515..10405
                     /locus_tag="VF633_12495"
     CDS             9515..10405
                     /locus_tag="VF633_12495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8471925.1"
                     /translation="MPSPSESVKIAIRRRINELFNDYERGERPALRRADALFATDSVA
                     WRVNGDIVTMMIGGVSGLLLQMLHPAVLAGVWDHSDFRADMHGRLRRTAKFIAVTTYD
                     HASKGRAAIDRVRKIHDKLGGTLPDGKTYRVSDPALLAWVHVTETISFLDSWVRYAEP
                     SMPAADQDAYFAEMARVGRALGADPVPTDRAGALALIQSMRPQLKADDRTREVAALVM
                     RQKVGGFAEDFAADIVMKAGADLLPDWARGMHGLPKATPVVRNGARTMQRTLNWTYTG
                     SPNRGIWPEEVLEWPDGNPR"
     gene            complement(10421..11536)
                     /locus_tag="VF633_12500"
     CDS             complement(10421..11536)
                     /locus_tag="VF633_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471926.1"
                     /translation="MSDRSVRNLGHLQASASTARVLNLLSVWKRHGNTEEWAAAPMFR
                     NAALNRCWIIKHRLRRNELDLFRSRRQVATKILLPIEATDLKLGGRAIFVGQVDFERS
                     LKATFGSSLDDGDLETLKLLDRLPSLDPFLLREQLKRHGIAPARCYFEVADADLKRMF
                     AFVEDEIRPLVTMSLGVSGGNGDVATASLVSKILSNAGGPEMEPLRQTLRLQADEYAE
                     GVFCWKGFLYYKWAVDNVLKQVSAVAAELTVIKPVGPMDAEARAYIERGRRVLGHLIM
                     RTCESVVNTLRVYDDAYANLTQNARPTAFREFLLDAPHLFTRLGEQIGAVEHIVSFWK
                     FRFGPNAAKVSVEELIDIMMDFEGSLAPRDEDGRPTA"
     gene            complement(11599..12228)
                     /locus_tag="VF633_12505"
     CDS             complement(11599..12228)
                     /locus_tag="VF633_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX8471927.1"
                     /translation="MADDTLDLQVRTADPDRWLSSRFVGDGEVRADLIALYAFETELL
                     SIPTRVTQPLLAEMRYVWWRDQMDGVFSGVTRKGHPVLEALTLAAGRRALARAPFDAL
                     IEAHIGRVHGEPHDLDAFYVGPMQAAVRILVGEGYDAAVVEAGRVRGLTQVGRAEDAS
                     ALRGKANATLKGLPAEAFPAVAHAALSGVERGDGARRLKLIWASMRGRI"
     gene            complement(12236..13219)
                     /gene="secF"
                     /locus_tag="VF633_12510"
     CDS             complement(12236..13219)
                     /gene="secF"
                     /locus_tag="VF633_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX8471928.1"
                     /translation="MTWWPLIKILPQKTNLHFVKYARYAAVLSVVLCVASIIGCFYPG
                     LNMGIDFRGGASLEVSKPAGQTLNVEAIRSAVSRLEIGDVGVQGIARLDTNVDDGSTA
                     IVRFQVPEGRDQTAVVNQVEGAITSATGQVSYTGVSVVGSKVSGELFTSGLLALGVAI
                     ALMFAYIWFRFEPQFGFGAVAGLLHDVILTFGLIVLLRLEFSLNMVAAILTVIGYSMN
                     DTVVVFDRLRENLRKYKSMPLRDVIDLSLNETLTRTIITGVTAVLVLAALAVFGGEAL
                     RGFSIALMFGIMVGTYSSIYVGAPIILLWGVKRGELNEDAKPVKLGMASRP"
     gene            complement(13216..14829)
                     /gene="secD"
                     /locus_tag="VF633_12515"
     CDS             complement(13216..14829)
                     /gene="secD"
                     /locus_tag="VF633_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX8471929.1"
                     /translation="MIQLSRWKIVLLALALFFGLFLAYPNVLTPAQRDALPGWLPKSA
                     LNLGLDLQGGSYLLLEVDVPAMRAKRVDNLAEDVRVTLRDAGINVNATQREEGGVVVT
                     LANASQSEAAYQALRRLVGGANQQGVVERAATRLGDNRLRFAFTQQAMDSMGASAVDQ
                     SIEVVRRRIDSLGTREPSITRQGANRIVVQAPGESDPAALERVIGQTAQLTFQMVDDE
                     NSVQQALAGAVPPDAVLMMDEDGITPLLIKRRVLVSGEQLTHADVTTDQNGQTAIGFR
                     FDGVGAQRFAQATGSANLGKRFAIILDGKVISAPRIQSTIANGSGIITGQFSIAQASE
                     MVNLLNGGALPAPLKVEERRTVTAELGADAVQNGKISTIIGFIIIVVFMLGTYGFLFG
                     GISVIGLVLNGLLIIAAMSLTQATLTLPGIAGLVLTFAVAVDANVLIYERMRDEARNG
                     RSVIASLDAGFNKAMGTIIDANLTTLVAAAIMFIFGAGPVRGFAWTLTIGVFTSVFSS
                     VLVAQVLLGYWLKVAKPKKLPIADAETVR"
     gene            complement(14864..15193)
                     /gene="yajC"
                     /locus_tag="VF633_12520"
     CDS             complement(14864..15193)
                     /gene="yajC"
                     /locus_tag="VF633_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX8471930.1"
                     /translation="MPTGEAGGLLPTILQFLPFVAIFVLFYFLLIRPQQKRAKEHAAL
                     ISAVKRGDTIVLSSGLIGKVTRVEDAEVNVEIAPSVNVRVVKGMIAEVRNRTAIAAND
                     TKAISKA"
     gene            complement(15313..16422)
                     /locus_tag="VF633_12525"
     CDS             complement(15313..16422)
                     /locus_tag="VF633_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8471931.1"
                     /translation="MNRPVYPGQPGQPYPGAVIVNEAPPPATAPISSIDGGGLPPAAV
                     TSSLNGGSGTPAYPAPRPTYPSPNYPAPVQAAPVQPTYVEPNAYAPPSYNPPGTPRAP
                     VTGGSVAYVIQPGDTISGVGRRFQTPVQTLIDLNALGPRGSISSGQRITLPDSAVDTG
                     PDPYATGPAPIGVTVPRAGFVPPPPTPPSGNAALPSARPVAPVSQRPVASAPFVPVRP
                     AQTTNPTQAPAQAAPQTAPVAGAIGLQWPVRGDIVRRFGPVGMGERNNGINIGAAAGA
                     PVMASAAGRVAYVGDALVGQGLTVLIVHREGWRTVYGHLGSATVDDGADVRAGQQIGT
                     VGLTAGDGRPSMHFETRRMQGDEPVAVDPLTVLPR"
BASE COUNT         3033 a         5581 c         5196 g         2635 t
ORIGIN      
        1 ggttgaaatg gatcggcgta agaggctcga acgcggcccg ggcgtcggcg agaatgacct
       61 cgaccatgcg ctggaccaga acccgttcga tcgtcgtgta ggggcgaccc tcgatgcgca
      121 gggcggcggt gccgcgacgc ccgcccagca gcacgtcgac gatcgaatag atcagattgg
      181 aatcgacggt cagcaggccg taattgtcga gctcctcggc ccggaacacg ccgaggatcg
      241 ccggcagggg aatcgagttc aggtagtcgc cgaaccggat cgacgagatg ttgtcgagcg
      301 agacctcgac gttgtccgag gtgaaattcc gcaagctggt cgtcatcagc cgcacaagcc
      361 ggtcgaagac gatctcgagc atcggcaggc gctcgtagga caccagcgcc gagttgatga
      421 tggcgcggat gccggtccgc tcggaaccgt cgtcgtcgcc gaggtcgaag cccaacaggc
      481 tgtcgatctc gtcctgattc agaacgcgtt cggacgccgc gctcgggaac tcgtcgtttt
      541 caggttctgc gaagccggtg tcgctcatta ctgcaccagc atcttctcga tcaggaccgc
      601 atcgaccttg cccggagcgg cgataaggtt gacgcggcgc aggatctcgg tgcgcagctg
      661 atagctgccc gctgatccgg ccaggtcttc ggggcgcagt tcgcgcagga agccctggaa
      721 catgtcctgc atccgcgggg cttcttcctg caatgtcgca gccaattccg cgtcgtgcat
      781 ctccagcgtc agcttgagct ggaggaaggt cggcttgccg tcgggcgact ggatgttcac
      841 caccatgtct gggagagtgt agaagatgac tccgtcaggt ccctcggtga tgaccccggc
      901 ggtggcatct tcgccttccg cgcccttctc gccttccttc ttctcgccgt gaccgccctt
      961 cttctcttcc ttcttgccgt gttcggcggc cttctcctcg ccgtgctcgc cttcggcggc
     1021 ggccggcttg ggctgcatca ggaagaaggc cacgccgccc ccgaccagca ccagcaaaac
     1081 gactgcgccg atgatgatca acagcatcgg cagcttcttc ttggggggag cctcgccgtc
     1141 gacctcaagg ccatgagcga cggcgaggtc ggtcgcagcg tccttcgcgc cgcccttctt
     1201 cttgccaagc ttcaacatgc cgtcggaggt cccggatgat tccagactcc agatgcggcc
     1261 ggtttggtta acgcgagatt gaaatccggc aaatcttgcc gggtggcctg gggcctctcg
     1321 cgcccaaagc cccgcccggc ctgaattaac cctgttggca cggtcgttgc gtggtccctt
     1381 tgcgaaggag ctcgcttacc gtggaaaacg ccgcctatat cggactgtca cgccagatga
     1441 cgctgcgccg cgagctcgac atcgtggcca acaacatcgc caacgccaac acgaccggct
     1501 tcaaggtcga gcaacttctc gttggcacgg aggtcggcgc ccgcgctcgc aacgactcga
     1561 tcaagcccgg cgccagcttc gtgctggaca acggcgttgg tcgcgacttc ggtcaaggcg
     1621 ccctgcagca gaccggtcgc agcctcgatt tcggtatcga gggcgaaggc gccttcttca
     1681 ctgtcaacga cgggaccacc gacgcctaca cccgcgacgg ggccttcacg ctggatccga
     1741 ccggcaagct ggtcaccaag gccgggctgg cggttcaggg cgacggcggc gacattatcc
     1801 tcgatcccgc tcttggcgct gccgtcatcg gcgaggacgg cacgatcagc cagaacggag
     1861 cgcttgtcgg caagctgacg ctggcccggt tcgagaccct cggcgcgctg tccaaggaag
     1921 gtgacggcct ctaccgcaac cgctccaact cgacgccgat cgacgctacg ggcgccaaga
     1981 tcagccaagg ctcgctcgaa gcctccaacg tcaatcccct gatcgagatc accagcatga
     2041 tcgagatcag ccgcgcctac gaacgcgcca cccgcatgat cgaaaacctc aatgacctgt
     2101 ccaagcgcgc cgtcgagcgt cttggccggc cgaactagga gggccgttag atgcgcgctc
     2161 taagaaccgc agcctcgggc atggccgccc agcaactgaa cgtggaggtc atctccaaca
     2221 acatcgcgaa catgaacacc attggctaca agcgccaacg ggccgagttc caggacctga
     2281 tgtaccagaa cgtcgagcgg atgggcgcgc agtcgtccag cgcaggcacc gtcgttccga
     2341 ccggcatcca ggtcggcctc ggcgtcaagg cgggcagcgt ctaccggatc accgaacagg
     2401 gcacgcccag caacaccggc aacacctatg acgtcgccgt cgacggcaag ggctatttcc
     2461 agattaccct gccctcgggc gaaatcggct acacgcgggc cggcaacttc tccctcagcc
     2521 ccgaaggcaa tctggtcact gaggacggct atgctgtcga gccggccatc accatcccac
     2581 agaacaccac cgacgtctcg atctccaaga ccggccaggt ctcggcgatt atcgacgggc
     2641 agaccgaccc gcagatcgtg ggccagctcg agctcgccaa cttcttcaac gaagcgggtc
     2701 tggaagccgt cggtgacaac ctgctgatgg aaacggccgc ctcggggcct gcgacggtcg
     2761 ccgcccccgg cgaaccgggc ttcgggcaac ttctgcaggg ctataccgag tcctccaacg
     2821 tcgacgctgt cagcgaaatc agctcgctga tcatcgccca gcgggcctac gaaatgaact
     2881 ccaaggtcat ctccaccgcc gacgacatga tgtctgtgac cgcccaggtg aagagctaga
     2941 tcatgcgcat cgcgatcgca ttctgcctcg ctcttatcgc cgctcccgcg ttggctggcc
     3001 cagtcaccct acggccacag ccggtcgacg aggatggccg ggtcacgttc ggcgacatct
     3061 tcgacggcgc aggctcagcg gctaacgtcg tcatcggcac gcgcaccggc ccgtcggtcg
     3121 tgtttgaggc gggccagctt cagggtctgg cgctgcgctc gggtctgcag tgggccaacc
     3181 ctcgcggcct cagccgcgtt gtggtgcgtg aaggatccgc ctcgccagct gctccagccg
     3241 ctgtcctcac gcctgtctcc acggtcgcgg ccccttcccg tcctgctacg acccgcgcga
     3301 acccgaccgt cagctcagca tctgcggccc gtccgggcgc aacctccgat gtcctcacct
     3361 atgctcgcaa cctgagcacc ggcgacgtga tccagcccga agacgtcatc tggacgaccg
     3421 ttcaggctca catggctccc ggcggctcgc ctcaggacgc cgatcaggtc atcggcctca
     3481 gcgccaggcg cgccttgcgc gccggagcgc ctgtctcgtc tcgcgacctg gcctcgcctc
     3541 aggtcatcgc ccgtaacgat ctggtcgagg tcgcctatct cgtcggcggc gtgaagctga
     3601 ccatcaccgg gcgggccacc cgcaacgcct ccatcggcga ggccgtgtcg gtgctcaaca
     3661 ccacttcgaa ccgcaccatc gacgcggtcg cgaccggtcc cggcaaggcc gttgtcggcc
     3721 cggctgcgca gtccgcccgt tcaaatcccc aacaattcgc cgcccgctag ggaaatccac
     3781 gccatgcgta aactgctcat cctctctatc gccaccgcag ccctgtcgct gggcgcctgc
     3841 tctaccgccg tcgaggccat tcgcggtcct gaactcgcgc ccatcggcta tcccgccgct
     3901 ctcgtgccga ccagccaggc ctacctgccg tcgccagagg aacaggccgc cacacgcgcc
     3961 cgtcagaacg gcgcgatggc cgccagcgcc aacagcctgt ggcgcaccgg cgcccgcacc
     4021 ttcttcggcg atcagcgcgc gcgccgggtc ggcgacatcc tgaccgtatc gatctcgatc
     4081 gatgacaagg cgcagacgtc caactcgacc actcgcgccc gcaccaacga cgcctcgggt
     4141 ggggtgacca acttctttgg tttggaaaac agcctggggc gcgccttccc gggcggtttc
     4201 gatccctcga acatggttgg cctgtcgggt caaagcaacg cgacgggctc cggcgccgtc
     4261 agccgctcgg aaaaagtcga cctgaccatc gccgccgtcg tcactgacgt actttcgaac
     4321 ggcaacctcg tgatccaggg tcggcaagaa gtgcgcacca accgcgaagt ccgcgaactg
     4381 accgtcgccg gtatcgtgcg cccggaagat atttcctcgg gcaacaccat caaccacacg
     4441 cagatcgccg aagcccgcat cagttacggc ggtcgtggcg acatcagccg cgtccaggcc
     4501 accccggctg cccagtctct ggtcgagcgt ttcagcccgt tctaagagcc tgacaccgcc
     4561 cggcgacttg aggctggaca acaaaagggc cggtcgcgtc tgcgaccggc ccttttcatt
     4621 tgctgtgaaa gcacgaccta gatgtgggta aggcccttag ccgcgcgata ggctgtcatg
     4681 aacggcgcgg cgaggacggt catggacagc gcgaccgcca gcgccatcag cagcatcagg
     4741 ccgccgagcg cgacaagact ggcgggtgtc gcgccgccgc cgctcacgac gccgacggag
     4801 atcggcaggg cgacgattcc cagtagcaac atgacgatac aggccatgaa gccggcgatg
     4861 atcgccatgc ccagcaacga ccagaacaga cctgcggtgg cttgccaccc ggccttgagg
     4921 tcgatgcggc cctccgcgaa ggccgccgga accacgaaca ccagccggac cgagagatag
     4981 atgcccgcga tcatcgccgc gacctggatc agcagggcta cagcgccgat gcccgatgcg
     5041 agggccagcg ctgccagcga ctcaccggcc tggttcacga tcagggacac gatggtgacg
     5101 atcgccaggg cgccgagcgt gcgaagctct tctcgcccca gtcggagaaa gccgaacctg
     5161 tcacgaccgg tgttggtcat ggcccgatag acggcgggca tcaggatcgc ctgcgccacc
     5221 atgctgactg gaaccgtggc cagcgccgcg tagctgagct gggtttgcag cgcgagtgag
     5281 ggctgcagct tgccgcccga ggccattacc gcctgaaact cgcctgcgat ggggctgagg
     5341 atcggagcca ccatcagata catggcgaac agcgaaatca gggccaccaa ggcccaggcg
     5401 aggaccgcga ccggatggtg gcgtgtcacc ctgaagcctt cgaaggcggc ttccgttgcc
     5461 gaaaaactca tcgctgcact cccgggcgac tctctagcga ccgctcatag cgcaggcttc
     5521 gggtcggcgt cgccctcgtt cggaatttca gcggcgtcca tcaactgctt gcacgcgctg
     5581 gcgaccggag cgccgaacat ggcgtggcta aagccgtcga acagagccag cggaagggcc
     5641 agcaccaaag ccgggaccag gaacggccga ccgaactcgg tcagatcggc aaagctatga
     5701 atgtcgttcg gattgggcag cgacacgtga agcatcaagg cggcgatgaa gaagacggtg
     5761 atgacaaggg ctccgatcac caaggtcaca gccatcagga tcagccacgc cagaacgccc
     5821 gtcgccgcga gacgccagcc ctgccccttg cccagcttcc aaccctgctc gaacgcgatg
     5881 gtgtgcaggg cgagactggc ggggatgaac agcgtcgccc ggccagcggc ccacaacgcc
     5941 gctgcgaaga cggccagacc ataggcgatg gtcaccagca ccgcggtcag ttcactgacc
     6001 tgccacagaa tggcgcacag cacggccccg atcagcgtca ggacgaaggt cccgaaatag
     6061 aggccgacca cgatcaccag catcacaacc ccgacgtaaa cctcgtcgaa gccaagccgc
     6121 aggaacagga accggtcctt cgtaccgacc ggcgccatcg cagcccgcat cgcggcggtc
     6181 cagatgacga cgctaagcag caggccaacg aggttcatca gcccgagacc gccctgtatc
     6241 ggcaggaagt tggcaatgac ctgttccatc tcttccgggg tcgtggcctg accgaaaggc
     6301 aaggctacca acatcggaag caagagcgaa aaggccgcga tcgtcagcac aacccgcatc
     6361 gctccccacg cgaacagggc cagcggttga tgacgcagca gccggaacgg cgtctgcagc
     6421 gcctcgccga tcgcgaagcg cgccgtcatt gtcagacccg tcattgccag actcccagga
     6481 gttgatgcgt ctcgagccgc cggtaggctg cgccgaggaa ggcgctggtc agcggccact
     6541 ggattgcgac cagcatcagg atcgcgacga cacccgccgc cttgccggcc aaaacgccgg
     6601 ccaccaccag ccccagccag atcaggcccg gaaccgcaca ggcgatcagt cctgcaagca
     6661 gcggcaagat ggcgcgattg gtcagccccg tcgccgtcag cgaaatcatc tgcccgcgcc
     6721 ctaccgtagc cggcgcgtag agcgacagcc gcacggcgaa cagcaccggc gcacccagca
     6781 cgaccacggc gacaccagcc agcagcatca gcttccacgt cggcccgacg gcggcccagt
     6841 cgcgtgcctc gaccgcttct gcgttcaggc ccgcgccgcc gaacagggcc agcgccacca
     6901 gcatcagcaa tgccagcatg atggtcatga acaaggcgca cagcagggtc gcccccgcca
     6961 gccgcgcttc cggcttgccg agctgcagcc cgccacggcg caggcccgaa cgccgcgccg
     7021 tctccagatc gatggccgtt ccgatacggg tcgtggctcc gaaagccgcc agacccgcga
     7081 tcagggtcag cacgagcagt acggcctcga ccgcgcctga cagccgcagc aacacgacgc
     7141 cgagcgccag cgcggtcgtc agcgccagag ccggtcctgc cccacgcgcc atttcgccca
     7201 tgcgccccac ggcctcggcc aaggcttcgg ccaagtccag ccgcccggcc ttgttcaata
     7261 gactgctgtt cagcggcgat tccgccattc gctcccccgg gtatcgccgt cactctagcg
     7321 cctcagcgga cctgtgggcc agacgcttgc tgacgaaccg ggcagaccac actaaaaggc
     7381 ccgccatgac ttcgacttcc gacatcaaac ccattcacgt catcggcgga ggcctcgccg
     7441 gctccgaggc cgcctggcag atcgccaacg ccggcgtacc cgtcatcctg cacgaaatgc
     7501 gcggcgtgcc cggcgtgaag accgacgccc accacaccga cggtctggcc gagctggttt
     7561 gctccaactc gttccgctcg gacgactggg agcacaacgc cgtcggcctg ttgcatgccg
     7621 aaatgcggga actcggctcg atcatcatgg ccagcgccca cgaccatcag gttcctgcgg
     7681 gcggcgctct ggctgttgac cgcgacggct tctcccagtc ggtcacagcc aaactcgaag
     7741 cccatccgct gatcaccatc tgccgtgaag agatcgccgg actgccgccc gaagactggg
     7801 acaacgtcat cgtcgccacc ggccccctca cctcgcccgc tctcgccgag gccatcctga
     7861 agctgaccgg cgaggagtcg ttgagcttct tcgacgccat cgcccccatc gtccacgccg
     7921 agtcgatcga cttcgacatc gcctggcgtc agtcgcgcta cgacaaggaa ggcccgggcg
     7981 gagacgccgc cgcctacgtc aactgcccga tgaacaaggc cgagtacgag gccttcatcg
     8041 acgccctgat cgacggcccc aaggccgagt tcaaggagtg ggagaacgtc ccctatttcg
     8101 acggctgcct gcccatagag gtcatggccg agcgcggtcg cgagaccctg cgccacggcc
     8161 cgatgaagcc cgtcggcctc accaatccgc gcgacccgct ggtcaaggcc tatgccatcg
     8221 tccagctgcg tcaggacaat gcgctcggca ccctgttcaa catcgtcggc ttccagacca
     8281 agctgaagca cggcgcgcag gctgagacct tccgcatgat cccgggcctg cagaacgccc
     8341 agttcgcccg tctcggcggc ctgcaccgca atacctatct caacagcccg cacctgctgg
     8401 atcgtcagtt gcgcatgaag tccatgccgc gcctgcgttt cgctggtcag gtcacgggcg
     8461 tcgagggcta cgtcgagagc gccgccacgg gcttactggc cggtcgcctg gcggcggcgg
     8521 agcggttggg ccaaccgctg gaagcccctg aggcccacac cgccatcgga gccctggtcg
     8581 aacacatcac cggcggccac ctgaccgggt ccaagttcca gccgatgaac atcaactatg
     8641 gcctcctccc cccgctggaa acgccgaaag tcgacgaggc cggcgtgaag atcccgctca
     8701 aggaacgcgg ccgcgccaaa aagcggctga tgagcatccg ggcgattgag agtctcaaag
     8761 cctggcgcga tgcggcctga gggcggcttg ccgtatcgca aaacgacgtt atatatagcg
     8821 gatgattgct atcgttcgcg cgtttctggt tctggtggtc gttgcgatgg ccgcttccgc
     8881 gaatgctcgc gacaagagcc cttacccgcc ccagccaacc ctgatggatc gtcaggacct
     8941 ccgtcgcacc catgacggac gcccggtcta tgtgctcaac acggatttcc actactgcag
     9001 ccgggagtat ggattggtcg tgacggtgcc cagcacgttc gtgacagatc tgatttcgat
     9061 cccgaaactt gcacgctgga tcaagactgc cgactccccg ggttatcaag cagccatcgt
     9121 tcatgactgg ctctatgcga gtggcaatgg aagcgaagcc gaacgccgcc aggccgacta
     9181 catcttctat gaggcactgg tggactacga cgtcgatccg atggtccgcc tggccatgca
     9241 ccagatggtt cggaagttcg gcaaggacgg ctatgggctg aagggcgact ggcttttcgc
     9301 cgacgttcgg ctgcacaagg ccgcgcccga agcccttggg cggacggtcg cgccggcgat
     9361 gacgcccgat gcatcatgca gcgcgtttca gaacgcggta gagtcgggtg aggattatct
     9421 tgcccgaatc cggccagcaa ccgtcggcgg ctctcgtccg caatagctga aaagcggtct
     9481 atccaaacgg gtctgcggag gttaaccagc cccgatgccc tctccgtctg aatccgtaaa
     9541 gatcgccatc cggcgtcgga tcaacgagct gttcaacgac tatgagcgtg gcgaacggcc
     9601 ggctttacgg cgggctgacg ccctgttcgc gacggactcg gtggcgtggc gggtcaacgg
     9661 cgacatcgtc actatgatga tcggcggcgt ctcgggcctg ttgctgcaga tgttgcatcc
     9721 ggcggtgctg gccggcgtct gggaccactc cgacttccgc gccgacatgc acggacgcct
     9781 gaggcgcacg gccaaattca tcgccgtgac cacctatgac cacgcctcca aaggacgtgc
     9841 tgcgatcgac cgggtacgca agatccacga caagctcggc ggaaccctgc ccgacggcaa
     9901 gacctatcgc gtcagcgatc cggccctgct ggcatgggtg catgtgaccg agacgatcag
     9961 ctttctcgac agctgggtgc gctatgccga gccatcgatg cccgcggcgg atcaggacgc
    10021 ctatttcgcc gagatggctc gcgtcggccg ggcactcggc gccgatcccg tcccgaccga
    10081 tcgcgcgggc gcgctggccc tgatccagtc catgcgaccc cagctcaagg ccgacgaccg
    10141 cactcgcgag gttgcggcgc tcgtcatgcg ccagaaggtc ggcggcttcg ccgaggattt
    10201 cgctgcggac atcgtcatga aggccggcgc cgacctgttg cctgactggg cgcgaggcat
    10261 gcacggcttg ccgaaggcca cgcctgtggt tcgcaacggc gcgcggacca tgcagcgcac
    10321 gctgaactgg acctacaccg gatccccaaa ccgcgggatc tggcccgaag aggtgctgga
    10381 atggccggac ggcaacccac gatagggcgg agtcccgccg tcaggctgtg ggtcgaccgt
    10441 cctcgtcccg gggtgcgaga ctgccctcga aatccatcat gatgtcgatc aattcctcga
    10501 cgctgacctt ggcggcattc ggcccgaagc ggaatttcca gaaactgacg atgtgctcta
    10561 ccgccccgat ctgctcgccc agacgtgtga acagatgcgg cgcatccagc aggaactcac
    10621 ggaaggccgt gggtcgggca ttctgtgtca ggttggcata ggcgtcgtca tagacccgca
    10681 aggtgttcac gaccgactcg caggtcctca tgatgagatg ccccagaacc cggcgccccc
    10741 tttcgatata ggcgcgcgcc tccgcgtcca tgggcccgac cggcttgatc actgtcagtt
    10801 ctgcggccac tgccgacacc tgcttcagca cattgtcgac ggcccacttg taatagagga
    10861 agcccttcca gcagaacacg ccctcggcgt attcatcggc ctgcagtcgc agggtctggc
    10921 gcagcggctc catctccggg ccacccgcgt tggacaggat cttggacacg agactggcag
    10981 ttgcgacgtc gccgttcccg ccactgacgc cgaggctcat ggtcaccaga ggcctgatct
    11041 catcctcgac gaaggcgaac atcctcttca ggtcggcatc cgcgacttcg aaatagcagc
    11101 gcgcgggcgc gatcccgtgc cgcttcagct gttcgcgcaa caggaaggga tccagcgacg
    11161 gcagccggtc cagcagcttc aaggtctcca ggtctccgtc gtcgaggctt gagccgaagg
    11221 tcgccttcaa gctgcgctcg aaatcgacct gtccgacgaa gatcgctcgc ccgcccagct
    11281 tgaggtcagt cgcctcgatc ggcagcagga ttttagtggc gacctgacgc cggctccgga
    11341 acaggtccag ttcgttgcgc cgcaagcgat gcttgatgat ccagcagcga ttcagggccg
    11401 cgttgcggaa catcggcgcc gccgcccatt cctccgtgtt cccgtgtcgc ttccataccg
    11461 acagcaggtt cagcacccgc gcggttgagg ccgaggcctg aaggtgacca agattgcgga
    11521 ccgaacgatc actcatgcat taaccttggc cgttaaagat gacgatctga ttatccggcg
    11581 ccagccaccc ccgcggccct agatccgccc tcgcatactg gcccagatca gcttcagccg
    11641 tcgcgcgcca tcgccgcgct cgaccccgga cagagccgca tgcgccacag ccggaaaggc
    11701 ctcggcaggc agtcctttga gcgtcgcgtt ggccttgcca cgcaacgcgc tggcgtcctc
    11761 agcccgcccg acctgcgtca accctctgac gcggccggct tcgaccactg cggcgtcata
    11821 accttccccg acaaggatcc ggactgccgc ctgcatcgga ccgacataga aggcgtcaag
    11881 atcgtggggc tcgccgtgca cccgacctat atgggcttcg atcaaggcat cgaacggcgc
    11941 gcgtgccagg gcacgacggc cagccgccag cgtcagagcc tcgagcaccg ggtggccctt
    12001 gcgcgtcacg ccggaaaaca ccccgtccat ctggtcgcgc caccagacat aacgcatctc
    12061 ggccagcagg ggctgcgtga cgcgcgtcgg gatggacagc agttcagtct cgaaggcgta
    12121 gagcgcgatc agatccgcac ggacctcgcc gtccccgacg aaccggctgg acagccagcg
    12181 gtcggggtcg gcggtgcgaa cttgcagatc gagcgtgtcg tcggccaact taaccctacg
    12241 ggcgcgaggc catgcccagc ttgaccggct tggcgtcctc gttgagttca ccgcgtttca
    12301 cgccccaaag gaggatgatc ggcgcgccga cgtagatcga ggaataggtg ccgaccatga
    12361 tcccgaacat cagcgcgatc gagaagccgc gcagggcctc gccgccgaag accgccagag
    12421 ccgccagcac cagcacggcg gtgacgccgg tgatgatggt ccgcgtcagg gtctcgttca
    12481 gcgacaggtc gatcacgtca cgcagcggca tcgatttgta tttgcggagg ttctcgcgaa
    12541 gacggtcgaa caccacgacg gtgtcgttca tcgaatagcc gataacggtc aggatggcgg
    12601 cgaccatgtt caggctgaac tccagccgca acagcacgat caggccgaat gtcaggatca
    12661 cgtcgtgcag cagaccggcg acggcgccga agccgaactg gggttcgaac cggaaccaga
    12721 tgtaggcgaa catcagggcg atggcgacgc cgagcgccag caggcccgag gtgaacaact
    12781 cacccgaaac cttggagccg acgacgctga cgccagtata gctgacctga ccggtcgcgg
    12841 aggtgatggc gccctcgacc tgattgacga cggcggtctg gtcgcggcct tcgggaacct
    12901 ggaagcggac gatggcggtg gagccgtcgt cgacgttggt gtccagtcgg gcgatgccct
    12961 ggaccccgac gtcgccgatt tccagccttg agacggcgct gcggatggct tcgacgttca
    13021 gtgtctggcc tgcgggcttg gacacttcaa gcgaggcgcc gccccggaag tcgatgccca
    13081 tgttcaaacc gggatagaag cagccgatga tcgacgcgac gcacagcacg accgacagca
    13141 cggcggcgta gcgggcgtat tttacgaaat ggagattggt cttctgtgga aggatcttga
    13201 tcaggggcca ccaggtcatc ggacagtctc cgcatcggcg atcggcagtt tcttgggctt
    13261 ggccaccttg agccaataac cgagcagcac ctgggcgacg agcaccgagg agaagaccga
    13321 ggtgaacacc ccgatggtca gcgtccaggc gaagccgcgg acggggcctg cgccgaagat
    13381 aaacatgatg gccgccgcca ccagcgtcgt caggttggcg tcgatgatgg tgcccatggc
    13441 cttgttgaag ccggcgtcca gcgaagcgat caccgaccgc ccgttccgcg cttcatcacg
    13501 catacgctcg tagatcagca cgttggcgtc cacggcgacg gcgaaggtca gcaccagacc
    13561 ggcgatgccg ggcagggtca gcgtcgcctg ggtcagggac atggcggcga tgatcagcag
    13621 gccgttcagg accaggccga tcaccgagat gccgccgaac aggaagccgt aggtccccag
    13681 catgaagacc acgatgatga tgaagccgat gatcgtcgag atcttgccgt tctggacagc
    13741 atccgcgccg agctcggcgg tgacggtccg gcgttcctcg accttcagcg gagccggcag
    13801 ggcgccgccg ttcagcaggt tcaccatctc ggacgcctga gcgatcgaga actggcccgt
    13861 aatgatgccc gaaccgttgg cgatggtgga ctggatccgg ggggccgaga tcaccttgcc
    13921 gtcaaggatg atggcgaagc gcttgccgag gttggcgctg cccgtcgcct gggcgaaacg
    13981 ctgggcgccg acgccgtcga aacggaagcc gatggcggtc tggccgttct ggtcggtggt
    14041 cacatcggcg tgggtcagct gttcgccgga caccagcacg cgacgcttga tcagcagtgg
    14101 cgtgatgcca tcctcgtcca tcatcagcac ggcgtctggc ggaaccgcgc ccgccagcgc
    14161 ctgctgcacc gagttctcgt cgtcgaccat ctggaaggtc agttgggccg tctggccgat
    14221 gacgcgttcc agcgcggcgg ggtcgctctc gcccggggcc tgaaccacaa tgcggttggc
    14281 gccctgacgc gtgatcgaag gttcgcgggt gccgaggctg tcgatccggc ggcgaaccac
    14341 ttcgatcgac tggtccacgg cgctggcgcc catgctgtcc atggcctgct gggtgaaggc
    14401 gaagcgcagg cggttgtcgc ccagacgcgt cgcggcccgc tcgaccacgc cctgctgatt
    14461 ggcgccgccg accagacgac gcagcgcctg ataagccgct tccgactggc tcgcgttagc
    14521 cagggtgacg accactccgc cctcttcacg ctgggtcgcg ttcacgttga tgcccgcatc
    14581 tcgcagggtg acgcggacgt cctcggcgag gttgtcgacc cgcttggcgc gcatcgccgg
    14641 cacgtcgacc tccagcagca ggtaggaccc gccttgcagg tcgagaccaa ggttcagggc
    14701 gctcttcggc agccagcccg gcagggcgtc acgctgcgcg ggcgtcagga cgttcggata
    14761 cgccaggaac aacccgaaga agagcgcgag tgcgagaaga acgatcttcc agcgcgaaag
    14821 ctgaatcatg gaatcgacta tccgtttcgg cgacgcgagg tgatcaggcc ttggagatgg
    14881 ctttggtgtc gttggcggcg atggccgtgc ggttgcgcac ttcggcgatc atgcctttga
    14941 cgacgcgaac gttgacgctc ggcgcgatct cgacgttgac ctcggcgtcc tcgacgcggg
    15001 tgaccttgcc gatcaggccc gacgacagga cgatggtgtc gccccgcttc acggcggaga
    15061 tcagtgcggc gtgttccttg gcgcgcttct gctgcggacg gatcagcagg aagtagaaca
    15121 ggacgaagat cgccacgaac ggcaggaact gcaggatcgt aggcagaagg ccgccagctt
    15181 caccagtagg cataatcgtt agtccccgac gcgggcggcc tcagtcgggc gcccgttaaa
    15241 aatgaggccg gaacctaggg ccgacgaccc gacattgcaa cgcgacgttt aggtcaagac
    15301 gcttcgcctg cgtcagcgcg gcaacaccgt cagcggatcg accgccaccg gctcgtcccc
    15361 ctgcatgcgc cgcgtctcga aatgcatcga cggacggccg tctccggcgg tcagaccgac
    15421 ggtgccgatc tgctgacccg cgcgtacgtc ggcgccgtcg tcaacagtgg ccgaacccag
    15481 gtggccatag actgtgcgcc agccctcgcg gtggacgatc aggaccgtca ggccctgccc
    15541 taccagcgca tcgccgacgt aagcgacgcg cccggcggcg gaggccatga ctggcgcccc
    15601 cgcggctgcg ccgatgttga tgccgttgtt gcgctcgccc atcccgacgg ggccaaagcg
    15661 tcgcacgatg tcgccgcgca ccggccattg caggccgatg gctccggcta ccggcgcggt
    15721 ctggggcgca gcctgggccg gcgcctgggt agggttcgtg gtttgcgcgg gccggacggg
    15781 gacgaacgga gccgaggcga cgggcctttg cgagaccggg gcgacaggac gcgccgacgg
    15841 cagggccgcg ttgccggaag gcggcgtcgg cggcggcggt acgaaccccg cgcggggcac
    15901 tgtcactccg atcggagccg ggccggtggc gtagggatcg gggcctgtat cgaccgcgct
    15961 gtcgggcaag gttatgcgtt gtcccgacga gatcgagccg cgcggcccca gcgcgttgag
    16021 gtcgatcagc gtctgcaccg gtgtctggaa gcggcggccg acacccgaga tcgtatcgcc
    16081 cggctggatc acataggcta ccgatccgcc ggtcaccggc gcgcgcggcg ttcccggggg
    16141 attgtagctc ggcggagcat aggcattggg ctcgacatag gtcggctgca ccggggcagc
    16201 ctgcaccgga gccggatagt tgggtgaggg gtaggtcgga cgcggggccg gataggcggg
    16261 agtgccggag ccgccgttca gcgaactggt cactgccgca ggcggcaggc cgccaccgtc
    16321 gatcgagctg atcggtgcgg tcgcgggcgg cggggcttcg ttgacgatca ccgcgcctgg
    16381 ataaggttgg cccggctggc cgggatacac cggacggttc accggggcct gcccggcata
    16441 gggtg
//
LOCUS       DASVTM010000454         6008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1054201,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000454 DASVTM010000000
VERSION     DASVTM010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054201"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /gene="miaA"
                     /locus_tag="VF633_12530"
     CDS             complement(<1..742)
                     /gene="miaA"
                     /locus_tag="VF633_12530"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX8471932.1"
                     /translation="MSPPLITLIAGPTASGKSKLALELAERTGAVIINADSQQLYADL
                     RVLSARPSVEEETRVEHQLYGVADAADAWSVGRWTRAITPLLERLHEEGRPALIVGGT
                     GLYFTALTKGLADIPDVPLEAREEAGALYDTEGEAAFRLRLTTFDPPAAAAIAPGDRQ
                     RLTRAWAVARTTEKSLSDWQASTVPMLEPRTYDRIVVEPDRAALYANCDARVATMIAS
                     GALDEVKALAARELDPALPAMKAVGVREF"
     gene            815..1888
                     /locus_tag="VF633_12535"
     CDS             815..1888
                     /locus_tag="VF633_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX8471933.1"
                     /translation="MSIDRATVVTALDAISDPKSGQGLFASGLVKGLTVGEDRAGFAL
                     EVPRADVALYGPVRDAAEAALKTMPGMARVSVILTTETAPAAAPKRTAGLSPAAVDQT
                     RAKAPVPTDRPAHVRCVLAIASGKGGVGKSTVAVNLAAALSARGLSVGILDADVYGPS
                     LPTMLGLSGKPAYEDGAMVPHEAFGLKAMSVGLLTGAGDAMVWRGPMASQALTQMLTQ
                     TRWGTEAAPLDVLVVDLPPGTGDVQLTLIQKTPLDGAVIVSTPQEVALADARRAHALF
                     QKVNVATLGLIENMSGDIFGRGGAEAEAARLSVPYLGDLPLTAAVREGGDAGKPVVIA
                     DPSGDIAVRFAGFAEAVAKALNV"
     gene            1964..3061
                     /locus_tag="VF633_12540"
     CDS             1964..3061
                     /locus_tag="VF633_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX8471934.1"
                     /translation="MSVDTLFRPFEVKSLKLPNRIVMAPMTRSFSPNGIPTSDVSAYY
                     RRRAEGGVGLIVTEGTVVERPAARNDVGVPHFHGEALPEWKKVVEEVHAAGGKIAPQI
                     WHVGSAPGRGEDWAPEGKVDSPSGMTRPGKVKHTPMTEEDVADTIAAFGRSARATKDL
                     GFDAVEIHGAHGYLIDQFMWDGVNARDDKWGGPSIADRVRFAVEIVKAVREGVGEDMA
                     LILRLSQWKLQDYAARIAETPDQMAEWLAPLSEAGVDIFHCSQRRFWEPEFEGSDLNF
                     AGWAKKLTGKPTISVGSVGLDGEFIAAFGGEGSKPASLEGLIERMERDEFDLVAVGRA
                     LLADPQWVEKIRDGRTADLQNFERQNLGVLY"
     gene            3216..3959
                     /locus_tag="VF633_12545"
     CDS             3216..3959
                     /locus_tag="VF633_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8471935.1"
                     /translation="MFAVLVTTVWLSLIIGARYLLIAGAVWWALWGRATSHGLQLNRD
                     KPSRALIAHEIRFSLLSTPIYALPAAIALEAWKAGGTKLYLDPGAYPLWWLPISFVLL
                     LLVQDAHYYWTHRLLHRREVFKWAHAGHHRSREPSPFASFSFDPIEAALTAWLLPVLA
                     FLIPLNIWMLAVLLTVMTITAVMNHCGRELWPDHWVRTGPGAHLITATHHSRHHTHMK
                     TNYGLYFRLWDRMMGTDGMPEARHQADRQ"
     gene            complement(4049..4576)
                     /locus_tag="VF633_12550"
     CDS             complement(4049..4576)
                     /locus_tag="VF633_12550"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX8471936.1"
                     /translation="MLIPDIALIVARGRNGVIGRDGDLPWKLRSDLQSFKAITMGKPC
                     IMGRTTWESLPLKPLPGRLNLVLSRDLSYEERGKARGAVVCSSLDEAIDMARETAVDD
                     AIREVCVIGGTAIFEATLPKAKRLYITEVDAEPEGDAWFPAFDESKWREISNEAHEAG
                     EKDDHAFAFRVLERL"
     gene            complement(4641..4952)
                     /locus_tag="VF633_12555"
     CDS             complement(4641..4952)
                     /locus_tag="VF633_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HEX8471937.1"
                     /translation="MHLKTLSADVVRISDIYAAEHDIDRDKASSGDWALLKLQEELGE
                     LTAEHLRMSGRARGEADAQALHDEAADVMGMLLIYCERVGIDLEVAMQRKWLHWLEPK
                     A"
     gene            complement(4963..5814)
                     /locus_tag="VF633_12560"
     CDS             complement(4963..5814)
                     /locus_tag="VF633_12560"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX8471938.1"
                     /translation="MTVALATADFRTETASADHPEWQYLNLLRDILENGVERGDRTGT
                     GTLGVFGRQIRFDLSKGFPLLTTKKLHLKSIVVELLWFLRGETNVRWLQDNGCQIWNE
                     WADAEGELGPVYGKQWRSWAAPNGESIDQISRLIEGLKTNPNGRRHIVSAWNPADIED
                     MALPPCHCLFQFFVADGKLSCQLYQRSADVFLGVPFNIASYALLTMMMAQVVGLEPGE
                     FVHTFGDAHLYLNHIEQAKLQLTREPLPLPTMTIAPKTDLFGFEAADFVLSDYAPWPH
                     IKAAVAV"
BASE COUNT          982 a         2184 c         1809 g         1033 t
ORIGIN      
        1 agaactcgcg taccccgacg gccttcatgg cgggcagagc ggggtccagc tcccgcgccg
       61 ccagcgcctt tacctcgtcc agcgcgccgc tcgcgatcat cgtggccacc cgtgcatcgc
      121 agttggcgta cagcgccgcc cgatcgggct cgaccacgat ccggtcatag gtgcgcggct
      181 caagcatggg cacggtcgag gcctgccagt ccgacagcga cttttcagtc gtgcgcgcca
      241 cggcccaggc ccgggtcagc ctctgtcggt cccccggcgc tatggctgcc gcggccggag
      301 gatcaaacgt ggtcagccgc agccggaacg ccgcctcgcc ctcggtgtca tacagcgcgc
      361 cggcttcctc gcgcgcctcc agcggcacat cgggaatatc ggccaacccc ttggtcaggg
      421 ccgtgaaata cagcccggtt cctccgacga tcagcgccgg gcgaccctct tcgtgcaggc
      481 gctccagcag gggcgtgatc gcccgcgtcc agcgccctac cgaccaggcg tcggcggcat
      541 cggccactcc atacagctgg tgctcgacgc gggtttcttc ctcgaccgat ggccgggcgc
      601 tcagcacccg caggtcggcg tacaattgct ggctatcggc gttgatgatc accgcacccg
      661 tgcgctcggc cagctccagc gccagcttcg acttgcccga ggctgtgggc ccggcgatca
      721 gggttatcag gggtggtgac aaaggaacgc tcgcagcaac tgcccccggg cgatagaagc
      781 agcggacccc gcctgcaagc ctgagaacac gcctgtgtcc atcgaccgcg ccaccgtagt
      841 aactgccctc gacgccatct ccgaccccaa atcggggcag ggactgttcg cgtccggtct
      901 ggtcaagggc ttgaccgtcg gcgaggaccg cgccgggttc gcactcgagg tcccccgcgc
      961 cgacgtcgcc ctctacggcc ccgtgcgcga tgcagccgag gccgcgctga agacgatgcc
     1021 cggcatggct cgcgtttccg ttatcctgac caccgagact gcgccggctg ctgcgccgaa
     1081 acgcaccgcc ggtctgtcgc ccgccgccgt cgaccagacc cgcgccaagg ccccggtgcc
     1141 aacggaccgt cccgcccacg tccgctgcgt cctcgccatc gccagcggca agggcggcgt
     1201 cggcaagtcc accgtcgccg tcaacctcgc cgcagccctg tccgcgcgcg gcctgtcggt
     1261 aggcatcctc gacgccgacg tctacggccc ctcgctgccg accatgctcg gcctgtccgg
     1321 caagcctgcc tatgaggacg gcgccatggt cccgcatgag gcgttcggcc tgaaagccat
     1381 gtccgtcggc ctgctgaccg gcgcgggcga cgccatggtc tggcgcggtc ccatggcctc
     1441 gcaggccctg acccagatgc tgacccagac ccgctgggga accgaggccg ccccgctcga
     1501 tgtgctggtc gtcgacctcc cgcccggcac cggcgacgtc cagctgaccc tgatccagaa
     1561 gaccccgctc gacggcgccg tcatcgtctc cactccgcaa gaggtggcct tggccgacgc
     1621 ccgccgcgcc cacgccctgt tccagaaggt caacgtcgcc acgctcggcc tgatcgagaa
     1681 catgtctggc gacatcttcg gtcgcggcgg cgcagaagcc gaagccgccc gcctgtccgt
     1741 cccctacctc ggcgacctcc cgctcactgc cgccgtccgc gaaggcggcg atgcaggcaa
     1801 acccgtcgtc atcgccgacc cctccggtga catcgccgtt cgcttcgccg gattcgccga
     1861 agccgtcgcc aaggctctga acgtctaagt tttttacgag agcagggttt tcaaacgcaa
     1921 ccctaaccgc caccttgttc ctcacacacc cgcgaggaca tccatgagcg tcgacaccct
     1981 gttccggcct ttcgaggtca agtccctgaa gctgccgaac cgcattgtca tggcccccat
     2041 gacgcgctcg ttctcgccga acggcatccc gacatccgac gtctccgcct actaccgccg
     2101 tcgcgccgag ggtggcgtcg gcctgatcgt caccgagggc acggtcgtcg aacgtccagc
     2161 cgcccgcaac gacgtcggcg tgccccattt ccacggcgag gccctgcccg aatggaagaa
     2221 ggtcgttgag gaagtccacg ctgcaggcgg taagatcgcg ccccagatct ggcacgtcgg
     2281 ctctgcgccc ggacgcggcg aggactgggc tcccgaaggc aaggtcgaca gcccgtccgg
     2341 catgacccgc cccggcaagg tcaagcacac gccgatgacc gaagaggacg tcgccgacac
     2401 catcgccgcc tttggtcggt cggctcgcgc caccaaagac ctcggtttcg acgccgtaga
     2461 gatccacggc gcccacggct atctgatcga ccagttcatg tgggacggcg tcaacgcccg
     2521 cgatgacaaa tggggcggtc cgtccatcgc cgaccgcgtc cgcttcgccg tcgagatcgt
     2581 caaggccgtg cgcgaaggcg tcggcgagga catggccctg atcctgcgcc tgtcgcagtg
     2641 gaagcttcag gactacgccg cccgcatcgc cgaaaccccc gaccagatgg ccgaatggct
     2701 cgcacccctg tccgaggccg gcgtcgacat cttccactgt tcgcagcgcc gcttctggga
     2761 gccggagttc gagggctcgg acctcaattt cgcaggctgg gccaagaagc tgacgggtaa
     2821 gccgaccatc tccgtcggct cggtcggtct tgacggtgag ttcatcgcgg cgttcggcgg
     2881 cgagggctcc aaaccggctt cgctcgaagg cctgatcgaa cgcatggagc gtgacgagtt
     2941 cgacctcgtc gccgtcggcc gcgctcttct cgccgacccg caatgggtcg aaaagatccg
     3001 cgacggccga accgccgacc tccagaactt cgaacgccag aacctcggcg tgctttacta
     3061 gcttcctgat cctcccccat cagggggagg gggaccgctc ttgcggtgga gggggctcac
     3121 cgcgcacacc gcgtccgcat cagccccctc cgtcacctcg ccaagaggcg acgcgccacc
     3181 tcccccaacg ggggaggatt ggctacactg accctatgtt cgccgtcctc gtcactacag
     3241 tctggctcag cctcatcatc ggcgcccgct atctgctaat cgcgggcgcc gtctggtggg
     3301 cgctatgggg cagggcgacc tcacatggcc tccagctcaa ccgcgacaaa ccctcccgcg
     3361 cgctgatcgc ccacgagatc cgcttctcat tgctctcgac cccgatctac gccctcccgg
     3421 cggccatcgc gcttgaggcc tggaaggctg gcggcacgaa actctatctc gatccaggcg
     3481 cgtatccgct gtggtggctg ccgatcagct tcgtcctgct gctgctggtc caggacgcgc
     3541 attactactg gacccaccgc ctgctgcacc ggcgcgaggt gttcaaatgg gcccacgccg
     3601 gccatcaccg ttcacgcgag cccagcccct tcgccagctt ctcgttcgat ccgatcgagg
     3661 ccgccctgac cgcctggctc ttgccggtgc tggccttcct gatcccgctg aacatctgga
     3721 tgctggccgt cctgctcacg gtgatgacca tcacggcggt catgaaccac tgcggccgcg
     3781 agctctggcc cgaccattgg gtccggaccg gccccggcgc ccacctcatc accgccaccc
     3841 accacagtcg ccaccacacc cacatgaaga ccaactacgg cctgtatttc cggctgtggg
     3901 atcggatgat ggggacggat ggaatgcctg aagcgaggca tcaggcagat aggcagtagg
     3961 cagtaggcag taggcagtgt cgccgcctga acggtcgagg ccagcgcttt cttctactgc
     4021 ctactgccta tctgcctcgc ttcactgcct acaagcgttc gagaactcga aaggcgaacg
     4081 catgatcatc cttctcgcct gcctcatgcg cttcgtttga aatctcgcgc cacttgctct
     4141 cgtcgaacgc cgggaaccac gcatccccct cgggctcggc gtcgacctcg gtgatgtaga
     4201 ggcgtttcgc cttgggcagt gtggcctcga aaatggcggt gccgccgatc acgcagacct
     4261 ccctgatcgc gtcgtcgacg gcggtttccc gcgccatgtc gatggcttcg tcgaggctgg
     4321 agcagaccac cgccccgcgc gccttgcccc gttcctcata ggacaggtcg cggctcagca
     4381 cgaggttcag ccgaccgggc aggggcttca gcggcaggct ctcccacgtc gtccgcccca
     4441 tgatgcacgg cttgcccatg gtgatggcct tgaacgactg caggtccgag cgcagcttcc
     4501 acggcaggtc gccgtcgcgc ccgattacgc cattccggcc ccgggcgacg atgagggcga
     4561 tatcgggtat cagcatggtc agctccggtc tgcggcccaa tctttggccc cgcctttcag
     4621 ccggacctcg gctcccagac ctacgcctta ggctccagcc agtgcaacca tttgcgctgc
     4681 atcgccactt ccagatcgat cccgacccgc tcgcaataga tcagcagcat ccccatcacg
     4741 tccgccgcct cgtcatgcag cgcctgcgca tccgcctcgc cgcgcgcccg gcccgacatc
     4801 cgcaaatgct cggccgtcag ctcccccagc tcctcctgca acttcagcaa cgcccagtcg
     4861 cccgacgacg ccttgtcccg gtcgatgtca tgctcagccg catagatgtc ggagatccgg
     4921 accacgtccg ccgatagggt tttcaggtgc atggtcaggc gatcagacgg cgacggcagc
     4981 cttgatgtgc ggccacggcg catagtcgct cagcacgaaa tcggccgcct cgaacccgaa
     5041 cagatcggtc ttcggggcga tggtcatggt cggcagaggc aggggttcgc gggtcagctg
     5101 cagcttcgcc tgctcgatgt ggttcagata caggtgcgcg tcgccaaacg tgtgcacgaa
     5161 ctcgccgggc tcgaggccga cgacctgggc catcatcatg gtcagcagcg catagctggc
     5221 gatgttgaac ggcaccccca gaaacacgtc ggccgagcgc tggtacagct ggcagctcag
     5281 ttttccatca gccacgaaga actggaacag gcagtgacag ggcggcaggg ccatgtcctc
     5341 gatatcggcg ggattccagg ccgagacgat gtggcggcgc ccgttcggat tggtcttcag
     5401 cccttcgatc aggcgcgaaa tctggtcgat gctctcgccg ttcggagccg cccacgagcg
     5461 ccactgcttg ccgtagaccg gccccagctc gccctcggcg tcggcccatt cgttccagat
     5521 ctgacagccg ttgtcctgca gccagcgtac gttggtttcg ccgcgcagga accacagcaa
     5581 ctcgaccacg atcgacttca gatgcagctt ctttgtcgtc agcagcggga agcccttgga
     5641 cagatcgaac ctgatctggc gcccgaacac ccccagcgtc cccgtgccgg tccgatcccc
     5701 gcgctcgacc ccgttctcga ggatatcgcg cagcagattc agatactgcc actccggatg
     5761 atctgcggac gcagtttcgg tgcggaaatc ggctgtggcg agggcgacgg tcatggcggg
     5821 gagagtggcc cgcgaatcac tctggcgcca atgacaatgc ggcctcgctt tagtgaggct
     5881 gtgggtatcg ccagccagcg cttttgattt tccgctcatc cccgcgaacg cggggaccca
     5941 gtgctttggg tccatgacag gtgaaacaag gtgcgttgcg cattcttgct atcgttcagc
     6001 actgggtc
//
LOCUS       DASVTM010000455        12006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_949180,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000455 DASVTM010000000
VERSION     DASVTM010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949180"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VF633_12565"
     CDS             <1..546
                     /locus_tag="VF633_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8471939.1"
                     /translation="SAEFWWMKPSDLTPYGELDDALTPEERDAFLSGYGGQEGYDSFV
                     LSDLVFNEPARHLARLHARNGNPTYLYRFDVVSAAMNEPHAGATHASERQYVFDTLST
                     SPWATDARDQTAADVMSGYWTEFAKAGDPNGGGRIRWPDLRPEPDLLLEFTNDGPVVK
                     PVPEAGRLDLIEQLYGRRKLR"
     gene            complement(865..1296)
                     /gene="arsC"
                     /locus_tag="VF633_12570"
     CDS             complement(865..1296)
                     /gene="arsC"
                     /locus_tag="VF633_12570"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169507.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX8471940.1"
                     /translation="MDVVIYHNPACGTSRNALDLIRHVGIEPHVIEYLKTPPSRAMVA
                     QLVQRMNLSVREIVREKSTPYAELGLGDPLLSDDQLLDAIAAHPVLMNRPIVVSPKGV
                     RLCRPSELVLDLLPDEAPLTPFTKEDGEVVIDVDGRRVDHG"
     gene            complement(1303..1638)
                     /locus_tag="VF633_12575"
     CDS             complement(1303..1638)
                     /locus_tag="VF633_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8471941.1"
                     /translation="MDSEPAILALAALAQSTRLEAFRLLVRHEPDGLPAGEIADHLAV
                     PANTMSAHLGVLSRAGLITSERRSRSIIYRADLDRVRDLVLFLLKDCCEGRADLCQPL
                     LAALAPCCD"
     gene            1718..3001
                     /locus_tag="VF633_12580"
     CDS             1718..3001
                     /locus_tag="VF633_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015105 - arsenite transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic transporter"
                     /protein_id="HEX8471942.1"
                     /translation="MILAVLIFVVTITLVIWQPRGLKIGWSALAGALAALALGVVSIG
                     DIPVVWGIVWNATATFVAIIVISLLLDEAGFFEWAALHVGRWGGGHGRRLFVLFVLLG
                     AAVSALFANDGAALILTPIVIAILLALGYGPKATLAFVMAAGFIADTASLPLVVSNLV
                     NIVSADFFDIGFDRYAAVMVPVDLAAIAATLAVLLVYFGRDIPTAYDVERLARPATAI
                     KDRATFVAGWIVLALLLVGFFVPEPMGVPVSAVAAVGAVVLLVVAGRGSVISTTKVLR
                     EAPWQIVIFSLGMYLVVYGMRNAGLTDHIAGLLDRFAQGGVWGATFGTGFLTAFLSSI
                     ANNLPTVLIGALSIEASSATGVVREAMIYANVIGSDLGPKMTPIGSLATLLWLHVLDK
                     KGVKIGWGHYFKVGVVLTLPVLAVTLAALAIRLTI"
     gene            3484..4572
                     /locus_tag="VF633_12585"
     CDS             3484..4572
                     /locus_tag="VF633_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8471943.1"
                     /translation="MKAATIRDVARHADVSVASVSRALNGTSAVNESTRQRILHAIDV
                     LRYVPHSGARSLSTSRTDTVGVILPDLFGEFFSELIRGMDLAARAHGMHLIVSSSHDD
                     ADEASAAIRSMRGRVDGLIVLSPYMNAATLTASMAGQTPVLLMNGGAMDAARPSIVID
                     NRGGAITAVEHLLSLGRRRIAHISGPTGNLEAEARLLGYAEALAGTGVVSRVLDGDFT
                     QGSGYRAVSQMLAGGERPDAIFAGNDMMAVGALLALQEAGVRCPEDVAVVGFDDVPIA
                     ELVRPALTTMRIDIAGTGKHAMERLVALVKARNAGPGHGDVANEIVRPTLVVRGSTTS
                     IDTQFQSTPAEAKPTAPDQILLGEIQDV"
     gene            4574..7564
                     /locus_tag="VF633_12590"
     CDS             4574..7564
                     /locus_tag="VF633_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471944.1"
                     /translation="MNTRTRIVMGASALAMATLLATAGAANAQATNSTLRGVVYEGQN
                     REPGGTITATEVSSGFVSRARISDEGGYVLAGLRPGTYRIEVTSADGSTATDTVTLST
                     GQVGTLNLDVAPVSTAAADADSTQVGDIVVTGRRIFEVRTSEVATGVSQQQINNLPQI
                     NRNFLNFAALAPGVRVTEGDSERTISAGGQSAQAINVFVDGQNQKSTIIDGGVAGQDD
                     SRGNPFPQAGVQEFRVITQNFKAEYEQAGSAIITAVTRSGGNEFHGEIFGTYQNQDWI
                     GQDVFASRRGDEKPELSKKQYGFALGGPIIQDRLNFFVTYERKDEERANTVNLENPAY
                     ATQFGSELGTYAAPFEEDLLFGKLSWQISDRQKVEVSGTYRSENDIRSFGGQTSIQRA
                     EQTNTDIRSINARHTFSGDRFLNEVTYDYFSYAYNPTALNFNDYGRTYAIFRDNNGDT
                     PAFDFNLNSRVQTVFSTGGRSDNQYVEQESHTLKDDLTIPDLQWMGTHTIKMGVKYSM
                     QNYQVNKQFGRNPQFTYDLTGGSGLGGPNIPVAVELGAFVPAADVDNNVLGLYFQDDW
                     QITDKLELNLGIRWDYEDNAVNNDYTTPANIRDTLAKIQALPGYDFPAYFNPSDYISD
                     GDREAFTGAFQPRFGFSYDVFGDERTVVFGGAGRYYDRLGFNFAFDERFKPQQFNKQI
                     FFSQNGGTVSGVNTVAWNPAYLTAAGLDPLLAATPGAGEIFLVNNNAEPPRTDQFNFG
                     VRQKVGIFQTALTLAYSKTVNLSAWYIANAGSATGDRFSGPTPTSVGHSEFNNLIFFQ
                     NQDKEAEFRALYLTIDKPFDRDSNWGLNIAYTLSKATQNGSRDSGLTGFDFDYNTPGL
                     SPEYPSGTDERHRIVASGTLGLPYEFRLSGIATLGSGLPYNVFDCPRATNPNICWNGG
                     RPDKRAFLPGLNFAFRQIDLRLSKSFKTFEGQNIEFMVDAINIFDFHNYSSFDGGYNN
                     STYGLPNAQFQPTRSFQVGLRYTF"
     gene            7662..9098
                     /locus_tag="VF633_12595"
     CDS             7662..9098
                     /locus_tag="VF633_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX8471945.1"
                     /translation="MDRRLFLMGSTAAAAASAVAFPTLAAQRTARIPIKRLDPEIDEL
                     QQRTFQWFVDVTDRRTGLTPDRWPTKSFCSIAAVGFALSCWPVGVERGWMSRAEARER
                     TLTTVRFFHDLPQGPEETGTAGYKGFFYHFLDMETGLRFGRTELSTVDTALLVAGMLF
                     SARYFDANSADEREIRDKVQAIYERIEWPWAVVRPNRITMGWHPDTGFIASDWYVYNE
                     GMLILLLAMGSPTHPVDAGLWSEWVTSYASSFTERWGSWHLEFAPIFGHQYSHMFIDF
                     RGIRDAWMRGQAAMRDDNLDYFENSRRAVYAQQKYAADNVGGWGYSAEIWGLTACDGP
                     GDFKQTLAGREREFFSYSARGPGDRDDGTIAPTAAAASIAFAPEIVVPCVKAMKARFG
                     AAIYTKYGFLDSFNPTLNEVGHPLQHGRIEPGLCWVDGDYLGIDQGPIVMMVENHRSE
                     LIWRHMHGEPNLRRGLEKAGFTGGWLDA"
     gene            9095..10369
                     /locus_tag="VF633_12600"
     CDS             9095..10369
                     /locus_tag="VF633_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX8471946.1"
                     /translation="MTEFAPDRRTALGLLAGGAAALSGCGRDDRRTTLQFWAMGNEAS
                     NVGALMPEFERRNPDIRVVVQAQPWSSAHQKLLTAYAGESLPDVSQVGNTWISELTAI
                     GALSAIPAEANDLLTDQFDAVLGTMQIEGRPMATPWYVDTRLLYYRTDLLERAGYDAV
                     PQDWAEWKRSMHAIKKVAGEGNYAILLPVNEFEQLQTLGLQGDEPLLRDQDTRGNFAS
                     PSFVAALAFYKSLFDEGLAPLASSTQISNVWTEFGRGYFSYYFSGPWTIGDMKSKLPP
                     AIQPHWTTAGVPGPRGAGASAPGGSSLAVFKSSDKQAAAWKLIRFLSEPAVQARFNVL
                     SGDLPARQSAWAAPQVANDPYIAAFKGQLSRAKPLPKVPEWERISTEMQIVAERTVRG
                     EYTPESAGAEINRRADRLLEKRRWMIEQGRAA"
     gene            10366..11265
                     /locus_tag="VF633_12605"
     CDS             10366..11265
                     /locus_tag="VF633_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8471947.1"
                     /translation="MSSTVSKRERAAWGFVAPALIAIAIFFVIPVISALFLSLTDFDI
                     YALADLKNLRFVGLQNYERLLTNPLFWGAMRNTVWFSVLGVPLAIGASLFAAVILNAR
                     TVKWRPIWRVVFFAPYVTTLVATAVLWNYMLHTKYGVINWILTSVGLPAVDWLGSPAT
                     SVPAILMFVVWKIFGYNMLIFLAVLQTVPDDLYEAARIDGANAWKQFRHVTLPAIAPT
                     LLLVSIISIAGFFQLFAEPYVMTQGGPAQSTVTVLYFMYEEGFKWWNLGSASAVAFIL
                     FLCIFAVTMVQLWVSRKLGGDHT"
     gene            11262..>12006
                     /locus_tag="VF633_12610"
     CDS             11262..>12006
                     /locus_tag="VF633_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8471948.1"
                     /translation="MKARQVLLNVAVAAIGLIVLFPLAWMVSVSFMSTGEAATFPPPL
                     LPKVWTLEHYRDLFVNQGMGRYMWNSFALATLATVLALGFTVPAGYAFAKLTFRGRDR
                     LFQVLIGALVIPAQIGTLPLFLMLKSVGLVNTYAGALVPWLASIFGLFLVRQYALSIP
                     DEMLEAARVDGATEGQIFRRIVLPTLSPILVTLGLFVFLGSWNDFLWPLIVLTDQTNY
                     TLPVALAALSREHVQDVELMMAGAVITVAP"
BASE COUNT         2106 a         3755 c         3943 g         2202 t
ORIGIN      
        1 tcggccgagt tctggtggat gaagccgtcg gacctgaccc cctatggcga gctcgacgac
       61 gccctgacgc ccgaggagag agacgccttc ctgagcgggt acggcgggca agaagggtac
      121 gacagcttcg tgctcagcga cctggtgttc aacgagccgg cccggcatct ggcgcggctg
      181 catgcacgga acggcaaccc cacttacctc taccggttcg acgtggtctc ggcggcgatg
      241 aacgagccgc acgcgggggc gacccacgcg tccgagcggc agtatgtctt cgacaccctg
      301 agcacctcgc cctgggccac cgacgcgcgg gaccagacgg cggcggacgt gatgagcggc
      361 tactggaccg agttcgccaa ggccggcgat cccaacggcg gcggtcgcat ccgctggccc
      421 gacctgcgcc cggagcccga cctgttgctg gagttcacca acgacgggcc ggtggtcaag
      481 cctgtgcccg aggcggggcg gctggacctg atcgaacagc tgtacggacg gcgtaagctt
      541 cggtgaaatc cgtttcgcgg actcgaaaaa aactgagtcc agagagtcca aatttgaacg
      601 taacggccga ggtgaggcga ataagtcgct gatctttaag gatggatatc gcgatcacct
      661 gttggactct gcgccagcga gagtccaatc cggtcaggct gttccaatgt gcgtatcgcg
      721 cgatgtcaaa gacccaggag ggcatcctac cactgcgctg gtgcatgctg gaccgattgc
      781 cgagtttcag cagaaggcga ccggcttaca acgcgctagg gttgggtgac atgcgcgctc
      841 ggcagaggtc cggcgtcttg tcagtcagcc gtgatcgacc cggcggccat caacgtcgat
      901 gacgacttcg ccgtcctcct tggtgaaggg cgtcaggggg gcctcgtcgg gcagcaggtc
      961 cagaacgagt tcggaggggc ggcacagtct gacgcctttc ggcgacacga cgatggggcg
     1021 gttcatcagg acggggtgcg ccgcgatggc gtcgagcaac tgatcgtccg acagcaatgg
     1081 gtcgccaaga ccgagttcgg cgtagggcgt gctcttctcg cgcacgattt cgcgcaccga
     1141 caggttcatg cgttggacga gctgtgcgac catcgcgcgg ctgggcggag ttttcagata
     1201 ttcaatcaca tgcggttcga tgccgacgtg gcggatcagg tcgagcgcgt tgcgcgacgt
     1261 tccgcatgca gggttgtgat agatgacgac gtccatggtt cctcagtcgc agcagggggc
     1321 cagagcggcc agcaggggtt ggcagaggtc ggcgcggcct tcgcagcagt ccttgagcaa
     1381 aaacagcacc agatcacgca cccggtcgag gtcggcacgg tagatgatcg agcggctgcg
     1441 tcgctccgag gtgatcagac cggcacgcga cagcacaccc agatgcgccg acatcgtatt
     1501 ggcgggaacg gcaaggtggt cagcgatttc cccggcgggc aggccatccg gctcgtgtcg
     1561 gaccagcagg cggaaggcct ccagacgcgt cgactgggct agggcggcga gcgccagaat
     1621 ggccggttcg gagtccatca ttccaagata ctggaactat ggacggatgc aagctagacg
     1681 tctcgcatag acgcctgtcg tccttgagag tcgccgcatg atcctcgccg tcctgatctt
     1741 cgtcgtgacc atcactctgg tgatctggca gccccggggg ttgaagatcg gctggagcgc
     1801 gctcgccggg gcgctagcgg cattggcgct gggcgtggtg tcgatcggcg acattccagt
     1861 cgtctggggc atcgtctgga atgcgacagc gaccttcgtg gccatcatcg tcatcagcct
     1921 gttgctggat gaggcggggt tcttcgagtg ggcggcgctg catgtcgggc gctggggcgg
     1981 aggacatgga cggcggctgt tcgtgctgtt cgtcctgcta ggcgcggcag tctcggccct
     2041 gttcgccaac gacggcgcgg cgctgatcct gacgcccatc gttatcgcca tactgctggc
     2101 cctcggctac ggaccaaagg cgacgctggc cttcgtcatg gcggcgggtt tcatcgccga
     2161 caccgcaagc ctgccgctgg tcgtgtccaa tctggtcaac atcgtctcgg ccgacttctt
     2221 cgacataggc ttcgatcgct atgcggcggt gatggtcccg gtcgatctgg cggcgatcgc
     2281 ggcgacgctg gcggtattgc tggtctactt cggtcgcgac atcccgaccg cctatgacgt
     2341 cgagcggctc gcgcgtccgg cgacggcgat caaggaccgg gcgaccttcg tggcgggctg
     2401 gatcgttctg gcgctgctgc tggtcggctt tttcgtgccg gagccgatgg gtgtgccggt
     2461 gtctgcggtc gccgcggtcg gcgccgtcgt gcttctggtc gtcgccgggc gcggttcggt
     2521 gatcagcacg acaaaggtgc tgcgcgaggc gccctggcag atcgtgatct tctcgctggg
     2581 catgtatctg gtggtctatg gcatgcggaa cgccggcctg accgatcaca tcgccgggct
     2641 gctcgaccgg ttcgctcagg gcggcgtctg gggggcgacg ttcgggaccg ggttcctgac
     2701 cgccttcctg tcatccatcg ccaacaacct gccgaccgtc ctgatcggag ccttgtcgat
     2761 cgaggcgtcc tcggcgacag gcgtcgttcg cgaagccatg atctacgcca acgtcatcgg
     2821 cagcgatctg gggccgaaga tgaccccgat cggatcgctg gccacgctgt tgtggctgca
     2881 cgttctggac aagaagggcg tgaagatcgg ttggggccat tatttcaagg tcggagtggt
     2941 cctgaccttg ccggtgctgg ccgtgacgct ggcggcgttg gcgatccggc tgacgatcta
     3001 accgaggtcg acctgcaagg cagcgaccgg gaatcagcag tcgcggcaag ctgagccgtt
     3061 tcacgggttc gtcgtcgtgt gtgtcaccat ccggccctca ttcgcgaaac aaaggcgaac
     3121 ctcgcgcgat cagaaatgcg tcgcgccggt cgcatcttgt ccgtcgattg aaatatagat
     3181 cagcgcagca tctgacaaaa gtcggggcaa gacagttagt tggtggccca tctgcgtcac
     3241 cgtttctgct tgtctcatcg aattttccaa tgtcgtagaa ccgacacggc acattcggac
     3301 tggcgcggaa gaccgctgtt gtgacggaag tacggcactt gcgggagggg tagcgagtgt
     3361 ggcatgtaac cgcttacatc gtttttatcg acggaccttc gataagaagg ccggcacggc
     3421 gacttggcag ggatcgggaa tgcaatcagc aggcgcacag ccgtcgacgc ggggagcgac
     3481 ctgatgaagg ccgcgaccat ccgcgacgtc gcgcgccatg ccgacgtgtc cgtagcctcg
     3541 gtttcgcgcg ccctgaacgg cacgagcgcg gtcaatgaat ccacccgcca gcgcatcctt
     3601 cacgccatcg atgtgctgcg ctacgtgccg cattcggggg cgcgcagcct gtccaccagc
     3661 cgcaccgaca ccgtcggcgt gatcctcccc gacctgttcg gcgagttctt ctccgagctc
     3721 atcaggggca tggatctggc ggcgcgcgcg cacggcatgc atctgatcgt gtccagctcg
     3781 cacgacgacg ccgacgaggc gtccgccgcc atccgctcga tgcggggccg ggtggacggg
     3841 ctgatcgtcc tgtcgcccta tatgaacgca gcgaccctga cagccagcat ggccgggcaa
     3901 acgcctgtat tgctgatgaa tggcggggcg atggacgcag cgcggccctc gatcgtgatc
     3961 gacaacaggg gcggagcgat cacggcggtc gagcatctgc tgagcctcgg tcggcggcgc
     4021 attgcgcaca tttccggccc gacgggcaac ctcgaggccg aggcgcggct gttgggctac
     4081 gccgaggcgc tcgcggggac gggggtcgtc tcgcgcgtgc tggacggcga cttcacccag
     4141 ggctcgggct atcgcgccgt aagccagatg ctggcgggcg gcgagcggcc ggacgccatc
     4201 tttgcgggca atgacatgat ggccgtcggc gcgctgctgg ccttgcagga ggcgggcgtc
     4261 cgctgccccg aggacgtcgc ggtcgtcggc ttcgacgacg tgccgatcgc agagctggtc
     4321 cggcccgccc tgaccacgat gcggatagac atcgccggca ccggcaagca cgcgatggag
     4381 cggctggtcg ccctggtcaa ggcgcgcaac gcgggccccg gacacggcga cgtcgccaac
     4441 gaaatcgtgc ggccgacctt ggtcgtgcgc ggctcgacca ccagtatcga tacccagttt
     4501 caatcaaccc cggcggaggc aaaacccacc gcgccggatc aaatcctttt gggggaaatc
     4561 caggatgtct aggatgaaca ctcgcacccg tatcgtcatg ggggcctcgg ccctggccat
     4621 ggcgaccctg ctggcgacgg ccggcgcggc taacgcccag gcgacgaact cgaccctgcg
     4681 tggggtggtc tatgaaggcc aaaaccgtga gccgggcggc acgatcaccg ccactgaagt
     4741 ctccagcggc ttcgtctcgc gcgcccggat ttccgacgaa ggcggctacg tcctcgccgg
     4801 cctgcgtcca gggacttacc ggatcgaagt cacgagcgcc gacggctcga ccgcgaccga
     4861 cacggtgacc ctgtcgaccg gtcaggttgg cacgctgaac ctcgacgtcg ccccggtctc
     4921 gaccgccgca gccgacgctg actcgaccca ggtcggcgac atcgtggtga cgggccgccg
     4981 catctttgaa gttcgcacct cggaagtcgc gactggcgtg agccaacagc agatcaacaa
     5041 cctgccacag atcaaccgca acttcctgaa cttcgcggct ttggcacccg gcgtgcgcgt
     5101 cactgaaggc gacagcgagc gcaccatctc ggcgggcggc cagtccgccc aggcgatcaa
     5161 tgtcttcgtt gatggtcaga accagaagtc gaccatcatc gacggcggcg tggccgggca
     5221 ggacgacagc cggggcaacc cgttcccgca ggctggggtg caggagttcc gggtcattac
     5281 ccagaacttc aaggcagagt acgaacaggc cggttcagcc atcatcaccg ccgtcacccg
     5341 ctcgggcggc aacgagttcc acggcgaaat cttcggcacc taccagaatc aggactggat
     5401 cgggcaggac gtgttcgctt cgcgccgcgg ggacgaaaag cccgaactga gcaagaagca
     5461 gtacggcttc gccttgggcg gcccgatcat tcaggaccgc ctcaatttct tcgtcaccta
     5521 cgaacgcaag gacgaggagc gagcgaacac ggtcaatctg gaaaacccgg cctatgcgac
     5581 ccagttcggg tctgaactgg ggacctatgc cgcgccgttc gaggaagacc tgctcttcgg
     5641 caagctgtcc tggcagatca gtgaccgtca gaaggttgag gtcagtggca cctaccgcag
     5701 tgagaacgac atccgcagct tcggcggcca gacgtccatc cagcgcgccg aacagaccaa
     5761 taccgacatt cgttcgatca acgcccggca caccttcagc ggcgaccgct tcttaaacga
     5821 agtaacctac gactactttt cttatgccta caatccgact gcgctgaact tcaacgacta
     5881 cggccggact tacgcaatat tcagagacaa caacggcgat acgccagcct ttgacttcaa
     5941 ccttaacagc cgtgtgcaga cggttttctc caccggtgga cgaagcgaca atcagtatgt
     6001 cgagcaagaa agccacacgc tcaaagacga cttgaccatc cctgatctgc aatggatggg
     6061 cacccatacg ataaagatgg gcgtcaaata ctcgatgcag aactaccagg tgaacaagca
     6121 gttcggtcgc aacccacagt tcacctatga ccttaccggc gggagcggcc ttggcggccc
     6181 gaacatcccg gtcgcggtgg agctcggcgc ctttgtgcct gcggcggatg tcgacaacaa
     6241 tgtgctgggt ctctacttcc aggacgactg gcagatcacc gataagctgg agttgaacct
     6301 cggcatccgg tgggattacg aggacaacgc ggtcaacaac gattacacga ccccggctaa
     6361 catccgcgac accttggcca aaatccaggc gctgcccggc tacgacttcc cggcttactt
     6421 caacccgagc gactatatct cggacggtga ccgcgaggcc ttcacaggtg cattccagcc
     6481 gcgcttcggc ttcagctacg acgtgttcgg cgacgaacgc accgtcgtgt tcggcggcgc
     6541 cgggcgctac tacgaccgtc tcggcttcaa cttcgccttc gacgagcgtt tcaagcccca
     6601 gcagttcaac aagcagatat tcttctccca aaatggcggg acagtcagtg gcgtaaacac
     6661 ggtggcctgg aacccggctt atctgacggc ggcgggtctg gatcccctgc tggcggcgac
     6721 gccgggcgcg ggcgagatat tcctcgtcaa caacaacgcc gagccgcctc gcacggacca
     6781 gttcaacttc ggtgtccgtc agaaggttgg catcttccag accgcactga ccctagccta
     6841 ttccaagacg gtcaatttgt cagcctggta catcgctaac gcgggctcgg cgaccggcga
     6901 ccgtttctcc ggccctaccc cgacctcggt tggccactct gagttcaaca acctgatctt
     6961 cttccagaac caggacaagg aagcggaatt cagggctctt tacctgacca tcgataagcc
     7021 gttcgatcgg gactccaact ggggcctgaa tatcgcctac accctgtcga aggcgacgca
     7081 gaacggcagc cgcgacagcg gtctgacggg cttcgatttc gactacaaca cgccaggcct
     7141 gtcgcccgaa tatccgtcgg gtaccgatga gcgtcaccgc attgtggcct cgggcaccct
     7201 cgggctgcca tacgagttcc gcctgtcggg catcgccacc ctcggttcgg gcctgcccta
     7261 caatgtgttc gactgcccgc gtgcgaccaa tccaaatatc tgctggaatg gcggccgtcc
     7321 tgataagcgg gcctttctac cggggctgaa cttcgccttc cgccagatcg atctgaggct
     7381 ttcaaagagc ttcaaaacct tcgaagggca aaacatcgag ttcatggtcg acgccatcaa
     7441 catcttcgac ttccacaact attcgtcgtt cgacggcggc tacaacaact cgacctacgg
     7501 gctgccgaac gcccagtttc aaccaacccg cagcttccag gtcggtttgc gctacacgtt
     7561 ctgatcttcc ttcctgacta cctcggccgc cccctgttcg cagggggcgg ttttcttttt
     7621 tgacgtctca attcgtgcct gttttcgcgt aggagtaccc catggatcgt cgactgtttc
     7681 tgatgggctc cactgccgcc gctgctgctt cggccgtggc gtttccgacg ctggccgcac
     7741 agcgtacggc acggattccg atcaaacgcc tcgaccccga gatcgacgaa ctgcagcaac
     7801 gcacgttcca gtggttcgtc gacgtcacgg atcggcggac cgggctgacg cccgatcgct
     7861 ggccgacgaa atccttctgt tcgatcgcgg cggtgggctt tgcactgagc tgctggcccg
     7921 tgggcgtgga gcgcggctgg atgagccggg ccgaggcgcg cgagcggacg ctgaccaccg
     7981 tgcgcttctt ccacgacctg ccgcagggcc cggaggagac cggcacggcc gggtacaagg
     8041 gcttcttcta ccacttcctc gacatggaaa cggggctgcg gttcgggcgc accgaactgt
     8101 cgaccgtgga cacggcgctg ctggtcgcgg ggatgctgtt ctcggcccgc tatttcgatg
     8161 cgaacagcgc cgacgagcgt gaaatccgcg acaaggtgca ggcgatctat gagcggatcg
     8221 aatggccgtg ggcggtcgtg cggcccaacc ggatcaccat gggctggcat ccggacaccg
     8281 gcttcatcgc ctcggactgg tacgtctata acgaggggat gctgatcctg ctgctggcca
     8341 tgggcagccc gacgcatccg gtcgacgcag ggctgtggtc cgagtgggtg accagctacg
     8401 cctcgagctt caccgaacgg tggggcagct ggcacctgga gttcgcgccg atcttcggcc
     8461 accagtacag ccatatgttc atcgacttcc gcggcatccg agacgcctgg atgcgcggtc
     8521 aggcggcgat gcgcgacgac aacctcgact acttcgagaa cagccgccgg gcggtctatg
     8581 cgcagcagaa atacgcggcg gacaacgttg gcggctgggg ctattcggcc gaaatctggg
     8641 gtctgacggc ttgcgacgga ccgggcgact tcaaacagac gctggcgggc cgggagcgtg
     8701 agttcttcag ctattccgcg cgcggtccgg gcgaccggga tgacggcacc atcgccccga
     8761 cggcggcggc ggcctcgatc gccttcgcgc ctgagatcgt ggtgccttgc gtcaaggcga
     8821 tgaaggcccg gttcggcgcg gcgatctata cgaaatacgg cttcctcgac agcttcaacc
     8881 cgaccctgaa cgaggtcggc cacccgctgc aacatgggcg gatcgagccg ggtctgtgct
     8941 gggtcgacgg agactatctg ggcatcgatc aggggccgat cgtgatgatg gtcgagaacc
     9001 atcgctcgga gctgatctgg cgccatatgc atggcgagcc caacctgcgg cgcgggctgg
     9061 agaaggccgg gttcaccggc ggatggctcg acgcatgacg gagttcgcgc cggatcgacg
     9121 gacggcgctc ggcctgttgg ccgggggcgc ggctgcgttg agcgggtgcg gtcgggatga
     9181 taggcgcacg acgctgcagt tctgggccat gggcaatgag gcgtcgaatg taggggcgct
     9241 gatgcccgag ttcgagcgca ggaacccgga tatccgcgtg gtggtgcagg cccagccgtg
     9301 gagctctgcg caccagaaac ttctgaccgc ctatgccggg gagtcgctgc cggacgtcag
     9361 ccaggtcggc aacacctgga tctcggagct gacggccatc ggtgcgctgt cggctatccc
     9421 ggctgaggcg aacgacctgc tgaccgacca attcgacgcc gtgctgggga ccatgcagat
     9481 cgaggggcgg ccgatggcca cgccctggta cgtcgatacg cggctgctct actatcgcac
     9541 cgacctgctg gagcgggccg gatacgacgc cgtgccgcag gactgggccg agtggaagcg
     9601 gtcgatgcac gcgatcaaga aggtggcagg ggagggaaac tacgcgatcc tgctgccggt
     9661 caacgagttc gagcaactgc agactctggg ccttcagggc gacgagccgt tgctgaggga
     9721 tcaggacacg cggggcaatt tcgccagtcc gtcgttcgtc gcggcgctgg ccttctacaa
     9781 gagcctgttc gacgagggac tggcgccgct ggcctcctcg actcagatct ccaacgtctg
     9841 gaccgagttc ggacggggct atttcagcta ttatttcagc ggcccgtgga ccatcggcga
     9901 catgaagtcg aagctgccgc ccgcgatcca gccgcactgg accacggcgg gcgtgccggg
     9961 gccgcgcggg gccggggcgt cggcgcccgg cggctcgtcg ctggcggtgt tcaagtcgtc
    10021 ggacaaacag gcggccgcgt ggaagctgat ccgctttctg tccgaacccg ccgtgcaggc
    10081 gcggttcaat gtcctcagcg gcgacctgcc tgcgcggcaa tctgcgtggg ccgcgccgca
    10141 ggtggcgaac gacccctata tcgccgcctt caagggccag ttgagccgcg ccaaaccgct
    10201 gcccaaggtg cccgaatggg agcggatctc gaccgagatg cagatcgtcg ccgaacggac
    10261 ggtgcgcggt gaatacaccc ccgaaagcgc cggcgccgag atcaaccgac gcgccgaccg
    10321 gctgctggaa aagcgccgct ggatgatcga acaagggagg gcggcgtgag ttcgaccgtc
    10381 tccaaacgcg aacgggcggc gtgggggttc gtggccccgg cgttgatcgc catcgccatc
    10441 ttcttcgtca ttccggtgat ctcggcgctg ttcctgagcc tgaccgactt cgatatctac
    10501 gccttggcgg acctgaagaa cctgcggttc gtcggcctgc agaactatga gcggttgctg
    10561 accaatccgc tgttctgggg ggcgatgcgg aacaccgtct ggttcagcgt gctgggcgtg
    10621 ccgctggcca tcggggcgtc gctgttcgcg gcggtgatcc tgaacgcgcg gacggtgaag
    10681 tggcggccga tctggcgggt ggtgttcttt gcgccttatg tgacgacgct ggtcgccacg
    10741 gcggtgctgt ggaactacat gctccacacc aaatacggcg tgatcaactg gatcctgacc
    10801 tcggtcggac tgccggccgt ggactggctg gggagcccgg cgacgtcggt gccggcgatc
    10861 ctgatgttcg tggtgtggaa gatcttcggc tacaacatgc tgatttttct agccgttctc
    10921 cagaccgtgc ctgacgacct ttacgaagca gcgcgcatcg acggggcgaa tgcgtggaag
    10981 cagttccggc acgtaacgct gccggccatc gccccgacgc tgttgctggt gtcgatcatc
    11041 tcgatcgccg gtttcttcca gctgttcgcc gagccctatg tgatgaccca aggggggccg
    11101 gcgcagtcga ccgtcacggt gctctacttc atgtacgaag agggcttcaa atggtggaac
    11161 ctggggtccg cctcggccgt cgccttcatc ctgttcctgt gcattttcgc ggtgacaatg
    11221 gtgcagctgt gggtgtcgcg caaactgggt ggagaccaca cgtgaaagct cgccaagtat
    11281 tgctgaacgt cgcggtcgcg gccatcggcc tgatcgtgct gttcccgttg gcgtggatgg
    11341 tgtcggtcag cttcatgtcg actggcgagg ccgcgacgtt tccgccgccg ctgctgccca
    11401 aggtctggac gctggagcat taccgcgacc tgttcgtgaa ccagggaatg ggccgttaca
    11461 tgtggaacag cttcgccctg gcgacgctgg ccacggtgct ggcgctgggg ttcacggtcc
    11521 cggccggata cgccttcgcc aagctgacgt tcagggggcg cgaccggctg ttccaggtgc
    11581 tgatcggcgc cctggtgatc ccggcccaga tcggcaccct gccgctgttt ttgatgctga
    11641 agtcggtggg gctggtgaac acctatgccg gggcgctggt cccgtggctg gcctcgatct
    11701 tcggcctttt cctggtccgc cagtacgccc tgtcgatccc ggacgagatg ctggaggcgg
    11761 cccgcgtcga cggcgccacc gaggggcaga tctttcgccg catcgtcctg ccgacccttt
    11821 cgccgatcct ggtgacgctg gggctgttcg tgttcctggg cagctggaac gacttcctgt
    11881 ggccgttgat cgtcctgacc gaccagacca actataccct cccggtggcg ctggcggccc
    11941 tgtcgcgcga gcatgtgcag gacgtcgagc tgatgatggc gggggcggtg atcaccgtcg
    12001 cgccgg
//
LOCUS       DASVTM010000456         7737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_412713,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000456 DASVTM010000000
VERSION     DASVTM010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412713"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1194
                     /locus_tag="VF633_12615"
     CDS             208..1194
                     /locus_tag="VF633_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471949.1"
                     /translation="MLIAIVSALLSATTPSSADRTCRFAGEPRTWTAQALTAWDRLDR
                     RRLHIARPVTPVIALFDDKCVYRLVPSTRGDFRVGQRRYRVTADQHSGELTLPDGNTV
                     PAQRLAFASPMSDGGMFFTMALPSLWRADTDEVRDPRLLSMVVFMHEFTHTQQADGLG
                     SRIDALLANGLPEDADDDVVQDQFQARPGYASGWSAERDSFYASAQAEDPAAARVALR
                     RSVDQMAERRARWFTGADAIYADADDVFLTMEGSGNWSAWAWLTDPAGGRMAPDAATA
                     FIRGAGTHWSQDEGLGAMLAIDRLTPDWPSLAFGPAAATIDDLAARALAEDR"
     gene            complement(1191..1880)
                     /gene="phoB"
                     /locus_tag="VF633_12620"
     CDS             complement(1191..1880)
                     /gene="phoB"
                     /locus_tag="VF633_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX8471950.1"
                     /translation="MQPYILVMEDEDALATLLQYNLEKEGYDVVIASDGEEGLVQIDE
                     RQPDLVLLDWMLPKVSGIEVCRRLRGRAETRNLPIIMLTARGEETDRVRGLDTGADDY
                     LTKPFSMVELIARIRAVLRRIRPGLADDRVGHGDIVIDRVAHRVRRNGTEVHLGPTEF
                     RLLDHFMRHPGRVFSREQLLDAVWGSDVYVEARTVDVHVGRLRKALDIGESANPIRTV
                     RSAGYSLDLEG"
     gene            complement(1892..2623)
                     /gene="phoU"
                     /locus_tag="VF633_12625"
     CDS             complement(1892..2623)
                     /gene="phoU"
                     /locus_tag="VF633_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX8471951.1"
                     /translation="MNTHTVKAYGDELNQLTAEVARMGGLAEAQVADAVESVARRDIA
                     LARAVVDRDVKLDAMHRDIEKKAIRLIALRQPVASDLRKTLSAMKLATDLERTGDLAK
                     NIAKRALAVAETEPMQPLTRSIERMGRLVSMRLRDVLDAYTSGELDGALAVWASDDEV
                     DEHYNALFRELLTYMMGDPRTINACTHLLFMAKNLERIGDHATNIAETVHYEITGDEM
                     IGERPRGAPGASETGLTHTHILPDA"
     gene            complement(2620..3588)
                     /gene="pstB"
                     /locus_tag="VF633_12630"
     CDS             complement(2620..3588)
                     /gene="pstB"
                     /locus_tag="VF633_12630"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX8471952.1"
                     /translation="MKFPFFHSPAKAPDGREGGPVDPTETPRMGGQVLPVAVEPTAEP
                     APDSGFVSSGGAQTRVPTVTSTFAPAGPTKITARDVSVFYGDKQALFDVSLDIPDKTV
                     TAFIGPSGCGKSTFLRTMNRMNDTIPGCKVTGKIDMDGKDINAKAVDPVLLRAQVGMV
                     FQKPNPFPKTIYENVAYGPKIHGLTTNKGELDAIVEASLKRAGLWEEVADRLHSAGTG
                     LSGGQQQRLVIARAIAVNPEVILMDEPCSALDPIATAKIEELIDELRERFCIVIVTHS
                     MAQAARVSQRTAFFHMGRLVEHGNTEEIFTNPRERRTLDYITGRFG"
     gene            complement(3593..4912)
                     /gene="pstA"
                     /locus_tag="VF633_12635"
     CDS             complement(3593..4912)
                     /gene="pstA"
                     /locus_tag="VF633_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX8471953.1"
                     /translation="MTDATPDMLSPLAEGRAADEARVGRLQAGIKKRYAKERRFKMYG
                     VMAVSIAVGFLLLLLGRIIEQGHTAFYAHEITLPVYMDPARIDRAYPQGTNFELLIAE
                     QELARFGIPDDAKGTKARAMRSLFSSEVKFRAAALVRNNPNVIGQTVTIAAPTSDEAD
                     LYYKGQISIATPADQRRLSNEQIGWLDRMKSNGSVRTHFNGALFTNGDSTEPELAGVL
                     GAVVGSALMLLVTAIIAIPIGIGAAVYLEEFSPRNRLSDLIEVNINNLAAVPSIVYGL
                     LGVALFINWMHLPRSSPLVGGLVLALMALPTIIIATRSSLKAVPPSIREAALAMGASR
                     TQTVFQHVLPLAMPGVMTGTIISMAHALGETAPLLLIGMVSFVPGIPHSAVEPTGALP
                     SLVYIWENASERAFHERTAAAILVLLAFMIVMNAGAILLRRRFERRW"
     gene            complement(4905..6341)
                     /gene="pstC"
                     /locus_tag="VF633_12640"
     CDS             complement(4905..6341)
                     /gene="pstC"
                     /locus_tag="VF633_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX8471954.1"
                     /translation="MIWFFLLVVIALSALAFIGGRVLAAKRATAEGVKPHSRPTQHGL
                     YALIWVGAPSLLVLILAGVFADGIAHQALVAGASPEVEQLEPFRREAFFADANLVGSG
                     RQAQQIWPAPLAEQLTVQGRRAAEIHTTLAIGTGIAALIAAILGAIFVITQIRPDLRA
                     RNRVEGWIVGVLFACSAVAVLTTIGIVFSLLWDSLRFFQSVPVTEFLFGTKWSPQIAI
                     RADQVGSSGAFGAVPLFAGTFLIMIIAMCVAAPVGLFSAIYLSEYASRTSRAVVKPIL
                     EILAGVPTVVYGFFAALTVGPALRVFFNSIGDFLIGGPFNDVGLYLSLVQNQMALVAG
                     AVMGIMLIPFVSSLSDDILNSVPQSLRDGSYAMGATKSETVKRVLLPAALPGISGALL
                     LAMSRAIGETMIVTMAAGLAAKLTFNPLDTVTTVTVQIVTLLTGDQEFNSPKTLAAFG
                     LGLTLFLVTLMLNIVAQRIVQTYREQYD"
     gene            complement(6472..7737)
                     /locus_tag="VF633_12645"
     CDS             complement(6472..7737)
                     /locus_tag="VF633_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8471955.1"
                     /translation="MALALVVAAATWFASRRPEGRSGTVFSGIEDAPASSAATAPLLD
                     NVFEALNDPVLIISGGEADDIAGRRVVMANAAARDLLRIAREGALLVSVMRDPGVLEA
                     VDEALFGGVTKTTDYSAGGARARHWRAWVRPLPSPVEAPLAILALRDETDVRRMEMMR
                     VDFLANASHELRTPLASLTGFIETLKGHARDDAVARDRFLDIMATQAERMTRLVADLL
                     SLSRIELNEHIPPSGRVDLAKAASDVIDAVSVLSQERGVVITLQSEGGKVNIDGDRDE
                     IIQVIQNLVDNGIKYSPSGQSVEIVIRRDLNIDAAPGPRTDGLTRLPLLTPDREAGIL
                     YAAVTVRDHGPGIAREHLPRLTERFYRVEGQKSGGERPGTGLGLAIVKHIVNRHRGGL
                     TVVSAPGEGAAFSAYFPMSAVRPETSDAV"
BASE COUNT         1392 a         2443 c         2598 g         1304 t
ORIGIN      
        1 agcgcgcaga catggtggag gacgacggca agatccgcgt cacttgcgag tactgctcca
       61 cggtttacgc gctctcgccg gaagacgtcg caaccggttc ggagagcacg accgactgag
      121 cccttcagcg accatgcggc aaggtgtcag caaattcact tgagcgaaca ccgtcctctc
      181 gaccattagg acgaaacgga aaccttcatg ctcatcgcca tcgtttcggc cctgctatcg
      241 gccacgaccc cgtcctcggc tgaccgcacc tgccggttcg cgggcgagcc ccggacgtgg
      301 acggcacaag ctctgacggc gtgggatcga cttgaccgtc gacgtcttca catcgcccgt
      361 ccggtgacgc cggttatcgc tctgttcgat gacaaatgtg tctaccggct cgttccatcg
      421 acgcgcggcg actttcgtgt gggccaacgg cgctatcgcg tgacagcgga tcaacactcc
      481 ggggagctaa cgctgccgga cggaaacaca gtaccggccc agcggctggc cttcgcgtcc
      541 ccgatgtcgg acggagggat gttcttcacc atggcgctgc cgtcactgtg gcgtgccgac
      601 acggacgagg ttcgtgatcc acgactgctg tcgatggtgg tcttcatgca cgagttcacc
      661 cacactcagc aggctgacgg tctgggttcg cgcatagacg ccctcctcgc aaacggcttg
      721 cctgaagacg ccgatgacga cgtggtgcag gaccagtttc aagcgcgccc cggctacgcc
      781 tcaggctggt cggctgagcg cgacagcttt tacgcttcgg cccaggccga ggatccagcc
      841 gccgcccgcg tcgccctccg tcggtccgtc gatcagatgg ctgagcgaag ggcgcgatgg
      901 ttcacggggg ccgacgccat ctatgccgac gcggacgatg tcttcctgac catggaaggc
      961 agcggaaact ggtctgcatg ggcctggctg acggatccgg caggcgggcg catggctccc
     1021 gacgcggcga cggcgttcat ccggggagct ggaactcact ggagccagga cgagggtctg
     1081 ggcgccatgc tggcaatcga caggctcacg ccggactggc ccagtctggc gttcgggccg
     1141 gctgcggcga cgatcgacga tctggccgcg cgcgcgctgg ccgaagaccg ctagccttcc
     1201 agatccagcg agtaccctgc cgaccgcacc gtgcggatcg ggttggcgct ttcgccaatg
     1261 tcgagcgcct tgcgcagacg gccgacgtgg acgtcgacgg tgcgggcctc gacgtagacg
     1321 tccgaacccc agacggcgtc gagcagttgt tcgcggctga acacccggcc ggggtggcgc
     1381 atgaagtggt cgagcagccg gaactcggtc gggccgaggt ggacctcggt gccgttgcgg
     1441 cggacgcggt gagcgacgcg gtcgatgacg atgtcgccat ggccgacgcg gtcgtctgca
     1501 agacctggac gaatccggcg caggacggcg cggatgcggg cgataagctc gaccatcgag
     1561 aacggcttgg tcagatagtc gtcggccccg gtgtcgaggc ccctcacccg gtctgtctcc
     1621 tcgccgcgcg cggtcagcat gatgatcggc aggttgcgtg tctcggctcg gccacgcagg
     1681 cggcggcaga cctcgatgcc cgagaccttg ggcagcatcc agtcgagcag gaccagatcg
     1741 ggttgccgct cgtcgatctg gaccagaccc tcttcgccgt cggaagcgat gacgacgtca
     1801 tagccctcct tctcgagatt gtactggagc agggtcgcca gcgcgtcctc gtcttccata
     1861 accagaatgt agggctgcat gtccgtttcc ctcaggcgtc gggcaggatg tgggtatggg
     1921 tcaggccagt ctcggaagcg cccggcgcgc cacgggggcg ctcgccgatc atctcgtcgc
     1981 cggtgatctc atagtggacg gtctcggcga tgttggtcgc gtggtcgccg atccgttcga
     2041 ggttcttggc catgaacagc agatgggtgc aggcgttgat cgtacgcgga tcgcccatca
     2101 tgtaggtcag cagttcgcgg aacagcgcgt tgtagtgctc gtcaacctcg tcatccgaag
     2161 cccagaccgc cagagcgccg tccagttcgc cggacgtata ggcgtccagc acgtcgcgca
     2221 gccgcatcga caccagccgt cccatccgct cgatcgaacg ggtcaggggc tgcatcggct
     2281 cggtctccgc cacggccaga gcacgcttgg cgatgttctt ggccaggtct ccggtgcgtt
     2341 ccagatcggt ggccagcttc atcgccgaca gtgtcttgcg cagatctgac gcgaccggct
     2401 ggcgcagggc gatcaggcgg atggcctttt tctcgatgtc gcggtgcatg gcgtcgagct
     2461 tgacgtcacg gtcgacgacg gcgcgtgcga gggcgatgtc gcgacgggcg acggactcaa
     2521 cggcgtccgc gacctgagcc tctgccagac cccccatacg ggcgacctca gcggtcagct
     2581 ggttcagttc gtcgccgtag gctttgacgg tgtgcgtgtt caaccgaagc gccccgtaat
     2641 gtaatcgagg gtgcgccgtt cgcggggatt cgtgaagatc tcctcggtgt tcccgtgctc
     2701 gaccagcctg cccatgtgga agaaggccgt ccgctgcgac acccgcgccg cctgagccat
     2761 cgagtgggtg acgatgacga tgcagaaacg ctcgcgcagt tcgtcgatca gttcctcgat
     2821 cttggccgtc gcgatggggt ccagagccga gcaaggctcg tccatcagga tgacttctgg
     2881 attgaccgcg atggcgcggg cgatgaccag acgttgctgc tggccgcccg aaaggccggt
     2941 gccggcggaa tgcagccggt cggcgacctc ttcccacaga cccgcgcgct tcagcgaggc
     3001 ctcgacgatg gcgtcgagct cgcccttgtt ggtggtcagg ccgtggattt tcggaccgta
     3061 ggcgacgttc tcgtagatgg tcttggggaa tgggttcggc ttttggaaca ccatgccgac
     3121 ctgggcgcgc aacagcacgg gatcgacggc cttggcgttg atgtccttgc cgtccatgtc
     3181 gatcttgccg gtcaccttgc agcccgggat ggtgtcgttc atccggttca tggtgcgcag
     3241 gaaggtcgac ttgccacagc ctgacggacc gatgaaggcg gtgaccgtct tgtccgggat
     3301 gtcgaggctg acgtcgaaca gggcctgttt gtcgccgtag aagaccgaca cgtcgcgcgc
     3361 ggtgatcttg gtcgggcctg cgggtgcgaa ggtcgaggtc acggtaggga cgcgcgtctg
     3421 ggctccgccg gacgacacga agccgctgtc gggcgcaggc tccgccgtcg gttcaaccgc
     3481 gaccggcagg acctgaccgc ccatgcgcgg cgtttcagtg ggatcgacag ggccgccttc
     3541 gcgaccgtcc ggagccttgg ccggcgagtg gaagaatggg aatttcatac gtttaccacc
     3601 ggcgttcgaa gcgtcggcgc agcagtatcg cgccggcgtt catgacgatc atgaaggcga
     3661 ggagaacaag gatcgcggcc gccgtccgct cgtggaacgc gcgttccgac gcgttctccc
     3721 agatatagac cagcgacggc agggcgcccg tgggctcgac cgccgagtgc ggaatgcccg
     3781 gcacgaagct gaccatgccg atcagcaaca agggcgcggt ctcgccgagt gcgtgagcca
     3841 tggagatgat cgtgcccgtc atgacacccg gcatggccag cggcaggacg tgctggaaca
     3901 ccgtctgcgt gcggctggct cccatggcga gagcagcctc ccggatcgac ggcggcacgg
     3961 ctttcagcga ggaccgggtg gcgatgatga tggtcggcag cgccatcagg gccagcacca
     4021 gaccgccgac cagcggcgac gatcgcggca ggtgcatcca gttgatgaac agcgccacac
     4081 ccaacaggcc gtagacgatc gacggcacgg ctgcgaggtt gttgatgttg acctcgatca
     4141 ggtcgctgag ccggttccgg ggcgagaact cttccaggta cacggccgca ccgatgccga
     4201 tagggatggc aatgatcgcg gtgaccagca gcatcagcgc cgagccgacc actgcgccga
     4261 gcacgcccgc cagttccggc tctgtcgagt cgccgttggt gaacagggcg ccgttgaagt
     4321 gggtgcggac cgagccattg gacttcatcc ggtcgagcca gccgatctgc tcgttggaca
     4381 ggcgacgctg atcggccggc gtcgcgatcg agatctggcc cttgtaatag agatcggcct
     4441 cgtctgaggt cggcgccgcg atcgtcaccg tctggccgat gacgttcgga ttgttgcgga
     4501 ccagagccgc agcgcgaaac ttcacttcgg aagagaacag agatcgcatg gcgcgcgcct
     4561 tggtcccttt ggcatcgtcg gggatgccga accgcgcgag ctcctgttcg gcgatcagca
     4621 actcaaaatt ggtgccttgc ggataggcgc ggtcgatacg tgccgggtcc atgtaaaccg
     4681 gcaaggtgat ctcatgcgcg tagaaggccg tatggccctg ctcgatgatc cggccaagca
     4741 gcagcagcag gaagccaacg gcgatcgaga cggccatgac gccgtacatc ttgaaccggc
     4801 gctccttggc gtagcgcttc ttgatgcctg cctgcaggcg gccgacgcgg gcctcatcgg
     4861 ccgcacgccc ttcagccaag ggtgaaagca tgtcgggggt cgcgtcagtc atactgttcc
     4921 cgataggtct ggacgatgcg ctgggccacg atgttgagca tcagggtcac caggaacagg
     4981 gtcaggccga ggccgaaggc cgccagcgtc ttgggactgt tgaactcctg gtcgcccgtc
     5041 agcagcgtca cgatctggac ggtgacggtc gtgaccgtat ccagcggatt gaaggtcagt
     5101 ttggcagcaa gaccggcggc catggtcacg atcatggtct cgccgatggc gcgcgacatg
     5161 gctagcagaa gcgcgcccga aatacccgga agggcagccg gcagcaggac ccgcttgacg
     5221 gtctctgact tcgtcgcgcc catggcgtat gagccgtcgc gcaacgactg cggcaccgag
     5281 ttcaggatgt cgtctgacag cgaggacacg aacgggatca gcatgatccc catgaccgca
     5341 ccggcgacca gcgccatctg gttctgcacc agcgacagat acaggcccac gtcattgaac
     5401 ggcccgccga tgaggaagtc gccgatgctg ttgaagaaga ccctcagcgc aggcccaacc
     5461 gtcagcgcgg cgaagaagcc gtagaccacg gtcggaacgc cggcgaggat ttcaaggatc
     5521 ggcttgacca ccgcgcgcga ggtgcggctg gcgtattccg atagatagat ggctgagaac
     5581 agcccgaccg gcgcggccac gcacatggcg atgatcatga tcaggaacgt tcctgcgaac
     5641 agcggcacgg cgccgaaagc ccccgacgac ccgacctgat cggcgcgaat ggcgatctgg
     5701 ggcgaccact tcgttccgaa caggaactcc gtcaccggca cggactggaa gaaccgtagc
     5761 gaatcccaca gcagggagaa gacgatgcca atggtggtca ggacggcgac ggccgaacag
     5821 gcgaaaagga cgccgacgat ccagccctcc acgcgattgc gggcccgcag gtcaggacgg
     5881 atctgggtga tgacgaaaat ggcgccgagg atcgccgcga tcagggcggc gatccctgtc
     5941 ccgatagcca gggtcgtatg gatttcagcg gcgcgacggc cctgaacagt cagttgttcg
     6001 gccagaggcg cgggccagat ctgctgggcc tgtctgccgg acccgacgag attagcgtca
     6061 gcgaagaagg cctcgcgacg gaaaggctcg agctgttcga cctcgggcga agcgcccgcg
     6121 accagagcct gatgggcaat gccgtctgca aacaccccgg ccaggatcaa caccagcagc
     6181 gacggcgctc caacccagat gagggcatac aggccgtgct gcgtgggacg agaatggggt
     6241 ttgacgccct cagccgtagc gcgtttcgcg gcgagcacac gcccgccgat gaaggcgagc
     6301 gccgaaagcg cgataaccac cagaagaaag aaccagatca ttgaggtccg acagaatcac
     6361 aggaatccgc gcccggcggg ggccggctcg gtgaggccgg aaactgccct ttattggcaa
     6421 gcggattacg acgggtccat gacacttccg taacagcgca ggtcagccgg ttcagacggc
     6481 gtctgaagtc tccggccgga ccgccgacat cgggaaatag gcgctgaacg ctgccccttc
     6541 gccgggagcg ctgaccaccg tcagtccgcc ccggtggcga ttgacgatat gcttgacgat
     6601 ggcgaggccg agacctgttc ccggacgctc ccccccgctc ttctggcctt cgacccgata
     6661 gaaacgttcc gtcagccgcg gcaaatgctc acgggcgatg ccggggccgt gatcgcgaac
     6721 ggtgacggcg gcataaagta tccccgcttc ccggtccggc gtcagtagcg gcaggcgggt
     6781 cagtccgtcg gtgcgtgggc ccggggcggc gtcgatgttg aggtcgcggc ggatgacgat
     6841 ctccaccgac tggccggatg gcgaatattt gatgccgtta tcgacgaggt tctggatcac
     6901 ctgaatgatc tcgtctcggt cgccgtcgat gttcaccttc ccgccctcgg actgcaaagt
     6961 gatgacgacc ccgcgctcct gagacaggac actgacggcg tcgatcacgt ccgaggcggc
     7021 tttcgccaga tcgacgcggc ccgacggcgg aatgtgttcg ttcaactcga tccggctgag
     7081 cgacaacaga tccgcgacga ggcgggtcat ccgttccgcc tgggtggcca tgatgtcgag
     7141 gaagcggtcg cgcgccacgg catcatcgcg ggcgtggccc ttcagggtct cgatgaaacc
     7201 cgtcagcgaa gccagcgggg tacgcagctc gtggctggcg ttggcgagga agtcgacgcg
     7261 catcatttcc atgcgccgga catctgtctc gtcacgcagt gccagaatcg ccagtggagc
     7321 ctcgactggc gaaggcaacg gccgcaccca ggcgcgccag tgcctggctc tggcgccgcc
     7381 cgccgaatag tcggtcgtct tggtcacacc gccgaaaagg gcttcgtcta ccgcctccag
     7441 caccccggga tcgcgcatga cggagaccaa cagcgcgcct tcgcgcgcga tacgcagcag
     7501 atctcgcgcg gcggcattgg ccatgaccac gcgccgtcct gcgatatcgt ccgcctcgcc
     7561 gccactgatg ataagcacgg ggtcgttcag cgcctcgaac acattgtcga gcaagggggc
     7621 ggtcgccgcg ctggacgcag gagcgtcctc gatcccgctg aacaccgttc cggaccgccc
     7681 ctccggcctt cggctggcga accaggtcgc agcggcgaca acaagggcca gtgcaat
//
LOCUS       DASVTM010000457         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_310860,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000457 DASVTM010000000
VERSION     DASVTM010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310860"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..128
                     /locus_tag="VF633_12650"
     CDS             24..128
                     /locus_tag="VF633_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8471956.1"
                     /translation="MSDLRANFQEVKYVKPEASRKGSSEVYLVAMNKR"
     gene            133..1211
                     /locus_tag="VF633_12655"
                     /pseudo
     CDS             133..1211
                     /locus_tag="VF633_12655"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
     gene            1215..>1536
                     /locus_tag="VF633_12660"
     CDS             1215..>1536
                     /locus_tag="VF633_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471957.1"
                     /translation="MNVMLAVTLAIAATPLQTGAGAGAGAPSATPVSTLQGRPLSEVL
                     TRLGATRDPAPAITLQTAGGRMDLYPVYELMPATPEGQICDVRLSSPDVSRIASGFAV
                     VQDGR"
BASE COUNT          249 a          498 c          525 g          264 t
ORIGIN      
        1 agggcgggga cgcgggtcgg gtattgtctg acctccgggc caatttccag gaggtcaaat
       61 acgtcaagcc cgaggccagc cgtaagggct cgtcggaagt ttatctcgtg gcgatgaaca
      121 agcggtaggg cgatgagtga agccgccaaa gacaaagatg tcagggcggc gttcgccaaa
      181 caggcggcga tctgcacggc agcgggcgcg cccttcacgg gtcgcgtctg tggcttgatc
      241 tgcgaacggc tggatcggtc cgggcttatc gggcgacgtg tgctcgactg gccgggcaat
      301 ccctcgcacg agggcgacgc cttgccgcta aggctcgcgg gcgggctgca tgatctggcc
      361 cggtcagggg tggatgcgga gctgaccgct atctacccgc ccaacggagc gccggatgat
      421 gatcaggtct gggccgtgct gccacaggcg cttgtagacc atgccgacgc ggtcgatccg
      481 tggctagacg gcccgccaca gaccaatgaa gtcggtcggt ccgctggatt gatgccgggc
      541 tgctggtgct ggccgagcgt ttcggcctgc cgttcgatct gtacgaactc ggcgccagcg
      601 ccgggctgaa cagcctgctg gacggctata gcttcgatct cggcggcgtg aaggcaggcg
      661 accctgcctc tcctgtgcaa ctggctccgg tctggacggg atcgcctccg cccgccgcta
      721 tcgtgcgtgt cgtctcgcgt caggcagtcg atcgaaatcc gcttgatgtc accgctccag
      781 caactcgacg catgctgtca gcctacgtct ggaccgacca gcgcgaccgc ttggcccggc
      841 tgaatgcggc gctagacttg gccaccgcga ctccgcttga cctcgcgcgc gcagacgctg
      901 ctgactggct ggaggaaagg ctgacgctga cgccgcaaca gggcgtctgc cgggtggtca
      961 tgcacaccat cgccttccag tatttcccgc cgggggttca ggaccgcatc cggacccata
     1021 tggccgtggt cggctcgcaa tccaccactg atgcgccgct ggcctggctg acctttgagg
     1081 ccgaggaaga tgggttcgag cgtcgcccca tgctgcgcct gcggacttgg ccgggacaag
     1141 aagaggcggt cgcgctggcg cggggccagc cgcacggcgc ccgctacgac tggttgatgg
     1201 aaaagggata agtcatgaac gtcatgctcg ccgttaccct cgccatcgcc gccaccccgc
     1261 ttcagaccgg agccggggcg ggggccgggg ctccgtccgc gacgccggtc agcactctgc
     1321 aaggacgccc cctcagcgaa gtgctgacgc gcctcggcgc aacacgcgat ccggccccgg
     1381 cgatcacgct ccagaccgct ggcggccgga tggacctcta tcctgtttac gaactgatgc
     1441 cggctacgcc tgaaggccag atctgcgatg tccggctgag cagtcccgac gtgtcgcgca
     1501 tcgcctccgg ctttgcagtg gtgcaggacg gaaggg
//
LOCUS       DASVTM010000458         6282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_99324, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000458 DASVTM010000000
VERSION     DASVTM010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99324"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VF633_12665"
     CDS             <1..1845
                     /locus_tag="VF633_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HEX8471958.1"
                     /translation="KGLDALARLWTTALEIEIACGFAPDGRLARRRHAVRPVVLTLVG
                     GLDWLLARPLGTLDETEFVAASALFTAAASLTSSEIAQTLRPAADWPGAAETVLASLA
                     AREASLAHMQSDLGRAAALRTAQAAAAARTSGRRHCVIATMATDTERDLRLGFARLAA
                     VDVFQTRDGQIIRRLHPVLASAITTHGGDVEEAERWLLGRRTLVDAPGLSHAELATRG
                     FTEAELEAVEIALARVDRLEDAFTPPVLEAGFIRDVLGVDHDTAAQGGVLAVLASPEH
                     IAAATAYVFGHRELSGWQSAPGRLRTVFADLDAAAISLAHAAEPSSDVADTTSVELPS
                     HAGIASAMAMLTQGAREGRRAMRLSRRQAADQPLLIIPDAEPLRRSPEPVAAPQSTVT
                     EKIVERVVERDRTRRKLPDRRKGYIQKAAVGGHKVYIHTGEYDDGELGEIFIDMHKEG
                     AAFRSLMNNFAIAISIGLQYGVPLDEFVDAFVFTRFEPAGRVTGNDSIGSATSILDYI
                     FRELGVSYLDRRELANADAEPLDADGLGSGKADEMVPAAHFISKGFARGAAPDNLVVL
                     PFGKREPEPRKVSFANADACSACGDFTLQQRGAVRVCDSCGAAESKKG"
     gene            1939..2424
                     /locus_tag="VF633_12670"
     CDS             1939..2424
                     /locus_tag="VF633_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8471959.1"
                     /translation="MKKAIVLIASVLAVASPVLAQNAGQIASVRAGHDCRGCNLFQAD
                     LSGFEKRGLNLTGSRLRQSDMSLAVMNRTRFANADMRDVEAYGGVFSNSNFSGANLTN
                     ASFVGAYLEGASFAGATLTGVNFSGARLARATGLTQRQLSQACGDEATALPGSLRIPA
                     C"
     gene            2547..3386
                     /locus_tag="VF633_12675"
     CDS             2547..3386
                     /locus_tag="VF633_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8471960.1"
                     /translation="MAGASQAFAETRISIQDRDVARMVTLGGSCNRCELSGRNLSGAT
                     FTGANFLSATLVGANLRGAELTGSNFSQSDFSRANMTGAEMNGANFTAASFVDVSMNG
                     VEATGGVFARANMARANLNGAELNGVNMNGAILQDANLSGAEMFGATLANVNARNASF
                     QHAEMQASNMSAGDFSGANFTGATLDNARFAGGRFARTNFRGASLRRTDIRGVDLSQS
                     VGLSQSQVGQACADDATRLPRGLQMGNCRSRTVLRISPPAAPATPRPPVPPAQRQFVM
                     VAD"
     gene            complement(3439..5226)
                     /gene="aspS"
                     /locus_tag="VF633_12680"
     CDS             complement(3439..5226)
                     /gene="aspS"
                     /locus_tag="VF633_12680"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX8471961.1"
                     /translation="MSRMHAYRSHTCGALRSTDAGANVRLSGWVHRKRDHGGLLFIDL
                     RDHYGLTQLVFHPETPGFAAVERVRAESVIRVDGEVVARDASVINVNLPTGEIELRVT
                     NVEVLSEAAELPLPVFGEPEYPEEIRLKHRYLDLRRETLHKNIVLRSRVISSIRQRMT
                     GQGFLEYQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVSGFDRYFQ
                     IAPCFRDEDLRADRSLEFYQLDVEMSFVTQEDVFAAIEPVMHGVFEEFADGKTVDAYP
                     FVRIPYRQSIAKYGTDKPDMRNPIEMQDVSEHFRGGGFGLFNKILGADAKNAVWGIPA
                     PKGGSRAFCDRMNSWAQGEGQPGLGYIFWSDDNNAWGGPIAKNLGEATDGLMAALGMG
                     KGDAAFFAAGDPSVFYKFAGSARSKLGQDLNLISDDEFKFCWIVDFPMFEWSDEEKKV
                     DFSHNPFSMPQGEMEALQTQDPLDILAYQYDIVCNGYELCSGAIRNHKAEIMLKAFEI
                     AGYDASVVEEQFGGMLNAFRYGAPPHGGLAPGIDRIVMLLANQVAIREVIAFPLNQQG
                     QDLLMSAPAEAAERQYKELHIRSAVPIKV"
     gene            5377..5664
                     /locus_tag="VF633_12685"
     CDS             5377..5664
                     /locus_tag="VF633_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8471962.1"
                     /translation="MFLVEITYTHPIEEIEAKTAEHRAWLEAQIASGLLILTGPKVPR
                     TGGFLIARGGQTLEEITEAMKEDPFQAHGLADYRIVEFAAGKMNPALTDLV"
     gene            5777..>6282
                     /locus_tag="VF633_12690"
     CDS             5777..>6282
                     /locus_tag="VF633_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HEX8471963.1"
                     /translation="MTDQLRLPLQRDVPAGAAEFVRSASNEEAARVLERWPDAAGRVM
                     AICGPAGCGKSHLAAIWAERVGAVALNGAEAAHADPLDLEGRPVLLDIAQDADDETLF
                     HLINLAQADGGALLLVSRPSPRFWKVQVPDLRSRLDSIRVTAMEEPDDVVLAAILRAR
                     FAERSIAPG"
BASE COUNT         1040 a         2158 c         1985 g         1099 t
ORIGIN      
        1 aagggcctcg acgccctcgc ccgcctgtgg acgacggcgc tggagattga aatcgcctgt
       61 ggtttcgcgc cggacggacg tctggcgcga cgtcggcatg ccgtccgccc ggtcgtcctg
      121 acgcttgtcg gcggcctcga ctggctgctc gcccgtcccc ttggaaccct cgacgagacg
      181 gagttcgtcg cagcctctgc gcttttcact gccgccgcgt ccctgacctc cagcgaaatc
      241 gcccagacgc tgcgtccggc tgccgattgg cccggcgccg cagagaccgt tctcgccagc
      301 ctcgccgcgc gcgaggccag cctggcgcat atgcaaagcg atcttggccg ggccgccgcc
      361 ttgcgcaccg ctcaggcggc ggccgccgca cgcacgtccg gtcgccggca ctgcgtcatc
      421 gcgaccatgg ctacagacac cgaacgggat ctgcgcctcg gcttcgcccg gcttgccgct
      481 gtcgacgtgt tccagacccg ggacggccag atcatccgtc gtctgcatcc ggtgctggcc
      541 tctgcgatca ccacgcacgg cggcgatgtc gaggaggccg agcgctggct tctcgggcgt
      601 cgcacgctcg tggatgcgcc ggggctcagc catgccgagc ttgcgacacg cggtttcacc
      661 gaggcggaac tggaggcggt cgagatcgcc ttggcgcgcg tcgatcgtct cgaagacgcc
      721 ttcacccctc cggtgcttga agcgggcttc atccgcgacg ttctgggtgt cgatcacgat
      781 acggccgcac agggcggcgt tctggctgta ctggcctctc ccgagcacat cgcagccgcg
      841 acggcatacg ttttcggtca ccgtgaactg tccggctggc agtcggcgcc tggtcggctg
      901 aggacggttt tcgccgacct cgacgccgcc gccatttccc ttgcccacgc ggctgaaccg
      961 tccagcgacg tcgccgacac gacgtccgtc gaacttccaa gccatgcggg gatcgcctct
     1021 gccatggcga tgctgacgca aggcgcgcgg gagggacgtc gtgcgatgcg cctgtcccgc
     1081 cgtcaggccg ccgaccagcc actcctgata attcccgacg cggagccctt gcgcagatcg
     1141 ccagagccgg tggccgcacc tcagtcgacg gtgaccgaga agatcgtcga acgcgtggtc
     1201 gaacgcgacc gcacccgtcg caagctgccc gatcgccgta agggctatat ccagaaggca
     1261 gccgtaggcg gtcacaaggt ctatatccac accggcgaat acgacgacgg cgagctcggc
     1321 gagatcttca tcgacatgca caaggaaggc gccgccttcc gctcgttgat gaacaatttc
     1381 gccatcgcga tctccatcgg acttcagtac ggcgtgccct tggacgagtt cgtcgacgcc
     1441 ttcgtcttca cccgtttcga acctgcgggc cgcgtcaccg gcaacgactc catcggctcg
     1501 gccacctcga tcctcgacta catcttccgc gagctgggag tgtcctatct ggaccgtcgc
     1561 gagcttgcca acgccgacgc cgaaccgctg gatgccgatg gtctgggctc tggcaaggct
     1621 gatgagatgg tccccgccgc ccacttcatt tccaagggct tcgcccgggg cgcagcgccc
     1681 gacaacctag tggtcctgcc cttcggcaag cgcgagcccg agccccgcaa ggtctcgttc
     1741 gccaacgctg acgcctgctc cgcatgcggc gacttcaccc tccagcagcg cggcgccgtc
     1801 cgggtctgcg acagctgcgg cgcggcggaa tcgaagaagg gctagtcgct cgtctacaag
     1861 ccacggatcg taaacatctc tgcgttatgg gtcttttcaa agatcacagc gtcggctgag
     1921 ccggcccgga gcctgtccgt gaagaaagcc atcgtcctca tcgcctcggt gctcgccgtc
     1981 gcctcgcccg tcctcgctca gaatgccggt cagatcgcca gcgtgcgcgc cggtcacgac
     2041 tgccggggct gcaacctgtt tcaggccgac ctgtccggct tcgagaagcg cggcctgaac
     2101 ctgaccggtt cgcgactgcg tcagtcggac atgagcctgg cggtcatgaa ccgcacccgg
     2161 ttcgccaacg ccgacatgcg cgacgtcgag gcctatggcg gcgtcttctc caactctaac
     2221 ttctccggcg ccaacctgac caacgccagc ttcgtcggcg cctatctgga aggcgcgtcc
     2281 ttcgccggag cgacgctgac cggggtcaat ttctcgggtg ctcgcctggc gcgggccacc
     2341 ggtctgacgc aacgccagct cagtcaagcc tgcggcgacg aggccaccgc actgcccggg
     2401 tccttgcgca ttcccgcctg ctaagcgacc tgactgttac cgcgatttaa gtaggctttc
     2461 aggcacttca gcctcattga ggcgatgtga aacagatcgc agcactaatc cgcaacgcct
     2521 ccgcggccgc cctcgggatg gtcgtgatgg ctggcgcttc acaggctttc gctgaaaccc
     2581 gtatcagcat ccaggaccgg gacgtcgccc gaatggtcac gctcggcggg tcctgcaacc
     2641 gctgtgaact gtcgggccgc aacctgtctg gcgcaacctt caccggcgcc aacttcctca
     2701 gcgccaccct cgtcggcgcc aacctgcgcg gcgccgagct caccggctca aacttctccc
     2761 agagcgactt cagccgcgcc aacatgaccg gcgccgaaat gaacggcgcc aacttcaccg
     2821 ctgcatcctt cgttgatgtc tcgatgaacg gcgttgaagc cacgggcggg gtcttcgccc
     2881 gcgcgaatat ggcgcgcgcc aacctgaacg gcgccgagtt gaacggcgtc aatatgaacg
     2941 gcgccattct gcaagacgcc aacctgtccg gagccgagat gttcggcgcc acccttgcca
     3001 acgtcaacgc ccgcaacgcc tcgttccagc acgccgagat gcaagcctcc aacatgagcg
     3061 caggcgactt ctccggtgca aacttcacgg gcgccacgct cgacaatgcc cggtttgccg
     3121 gaggtcggtt cgctcgcacc aacttcagag gggcgtcgct gcggcgtacc gacattcgtg
     3181 gcgtggatct gtcgcaatcg gtcggtctga gccagtcaca ggttggacag gcctgcgctg
     3241 acgacgccac tcgccttccc cgcggcctgc agatgggtaa ctgccgcagc cgcaccgttc
     3301 tccgcatatc gccgcccgca gcaccagcca ctcctcggcc gcctgtgccg cccgctcaac
     3361 gccagttcgt tatggtggcc gactaaacaa aagggcggcc tcgcggccgc cctttccgtt
     3421 tctactgagg ctgcagcctc agaccttgat cggtacagcg ctgcggatat gcagttcctt
     3481 gtactgacgc tcagccgcct cggcgggggc gctcatcagc agatcctggc cctgctggtt
     3541 cagcgggaag gcgatgacct cgcggatcgc gacctgattg gccagcagca tgacgatgcg
     3601 gtcgataccg ggggccaaac ccccgtgcgg cggcgcgccg tagcggaagg cgttcagcat
     3661 gccgccgaac tgctcttcga ccaccgaggc gtcgtagccg gcgatctcga acgccttcag
     3721 catgatctcg gccttgtggt tccggatcgc gcccgagcac agctcatagc cgttgcagac
     3781 gatgtcgtac tgataggcga ggatatcgag cggatcctgc gtctgcagcg cctccatctc
     3841 gccctgcggc atcgagaacg ggttgtgcga gaaatcgacc ttcttctctt cgtccgacca
     3901 ttcgaacatc gggaagtcga cgatccagca gaatttgaac tcgtcgtccg agatcaggtt
     3961 caggtcctga cccagcttgc tgcgggccga acctgcgaac ttgtagaaca ctgacgggtc
     4021 gccggcggcg aagaaggcgg cgtcgccctt gcccatgccc agcgcagcca tcaggccatc
     4081 ggtcgcctcg ccgaggttct tggcgatcgg tccgccccaa gcgttattgt cgtccgacca
     4141 gaagatgtag ccgaggcccg gctggccttc gccctgggcc caggagttca tgcgatcgca
     4201 gaaggcgcgc gagccgccct tgggcgccgg gatgccccag acggcgttct tggcgtcggc
     4261 gccgaggatc ttgttgaaca ggccgaaacc accgccgcgg aaatgttcgg acacgtcctg
     4321 catctcgatc gggttgcgca tgtcaggctt gtcggtgccg tatttggcga tcgactgccg
     4381 gtacgggatg cgcacgaacg gataggcgtc gacggtcttg ccgtcggcga actcctcgaa
     4441 cacgccatgc atgacgggct cgatggcggc gaagacgtct tcctgggtga cgaagctcat
     4501 ctcgacgtcg agctggtaga attccagcga acggtcggcg cgcaggtcct cgtcgcggaa
     4561 gcagggcgcg atctggaaat agcggtcgaa gcccgagacc atcagcagtt gcttgaactg
     4621 ctgcggcgcc tgcggcagcg cgtagaattt gcccggatgc agacgcgacg gcacgaggaa
     4681 gtcgcgcgct ccttcggggc tggaggccgt caggatcggc gtctggtatt cgaggaagcc
     4741 ctgccccgtc atgcgctggc ggatcgagga gatcacgcgc gaacgcagca cgatgttctt
     4801 gtgcagggtc tcacggcgca ggtcgagata gcggtgcttg aggcggattt cctcggggta
     4861 ttcaggctcg ccgaagaccg gcagcggcag ttcggcggct tccgacagga cctcgacgtt
     4921 ggtcacacgc agctcgatct cgccggtcgg caggttgacg ttgatcacgg aggcgtcgcg
     4981 ggccacgacc tcgccatcga cccggatcac gctctcggcg cgcacgcgtt cgacggcggc
     5041 gaagcccggg gtctcggggt ggaagaccag ctgagtgagg ccatagtggt cgcgcaggtc
     5101 gatgaatagc agacccccgt ggtcgcgctt gcggtggacc cagcccgaaa ggcgcacatt
     5161 ggcgcctgcg tccgtcgatc ggagggcgcc gcaggtgtgt gagcgatagg cgtgcatgcg
     5221 tgaaatcgtc tgaaaacggc cgctgaggcc gggaattgga aggcgcggaa gggcgcatca
     5281 tcgacccgga aagtcaaggt cggagccgat ttcgcggcct tgttcatggc cccgcgaatc
     5341 cacatattaa cgagcgtcac agtcaggaag tccgccatgt tcctcgtcga aatcacctac
     5401 acgcatccca tcgaggaaat cgaggcgaag acggccgagc atcgcgcttg gctggaagcc
     5461 cagatcgcca gcggtctgct gatcctcacc ggtcccaagg tcccccgcac tggcggcttc
     5521 ctgatcgcgc gtggcggcca gacgctcgag gaaatcaccg aggcgatgaa ggaagacccg
     5581 ttccaggccc acggccttgc cgactaccgg atcgtcgagt tcgcggcggg caagatgaac
     5641 cctgccctga ccgatctggt ctgagccgcc cacaggccca cggtgttgtc cccgatgtcc
     5701 ccggcacgac ggttcggccg ttaaccgcgt cgctcgcggg gggtgctagg gtgtcgccat
     5761 gccgggccgc ctcgccatga ccgatcaact ccgcctgccc ttgcagcgcg acgtgcccgc
     5821 cggcgcggcg gagttcgtgc gttcggcctc caacgaggag gcagcgcgtg ttctggaacg
     5881 ctggcctgac gccgccggac gcgtgatggc catttgcggt cccgccggat gcggcaaaag
     5941 ccatctggct gctatctggg ccgaacgcgt cggcgccgtg gccttgaacg gggccgaggc
     6001 cgcccacgcc gacccgctgg atctcgaagg ccgtccggtc ctgcttgaca ttgcgcagga
     6061 cgccgacgac gagaccctgt tccacctgat caacctggcc caggccgacg gcggcgccct
     6121 tttgctggtg tcgcgcccct ccccccgatt ctggaaggtt caggtccccg acctgcgctc
     6181 ccgcctcgac tccatccgtg tcacggcgat ggaggaaccg gacgacgttg tcctcgccgc
     6241 catccttcgc gcccgattcg ccgagcgcag catcgcgccg gg
//
LOCUS       DASVTM010000459         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_311073,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000459 DASVTM010000000
VERSION     DASVTM010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311073"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VF633_12695"
     CDS             complement(<1..645)
                     /locus_tag="VF633_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX-like domain-containing protein"
                     /protein_id="HEX8471964.1"
                     /translation="MSLPFLNTFAGNPLDRAGDLRNDPSWLAEQAEKVDALAMVLWEG
                     KPLLEVGPEGPRLAWVSLPHARDLVKDRELFLGLWKDAPVFAVEFESPVDPADAAVHG
                     LGRFTEMREAAAVLPGAEAAMAGTAKSLFDWRRRHGFCAACGNASDNGNGGWKRVCPA
                     CGTEHFPRVDPVTIMLPVYKGGPEPICLLGRQAAWPAGRMSALAGFLEPGESIEE"
     gene            721..820
                     /gene="ffs"
                     /locus_tag="VF633_12700"
     ncRNA           721..820
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF633_12700"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1101..1505)
                     /gene="apaG"
                     /locus_tag="VF633_12705"
     CDS             complement(1101..1505)
                     /gene="apaG"
                     /locus_tag="VF633_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX8471965.1"
                     /translation="MHDTPAYTAETDGILIKVRPSYLAGQSDPEAGRWVWAYQVEIIN
                     LGMSTVQLVSRRWVITDARGHVEHVEGPGVIGEQPVIAPGDSYSYASGCPLGTPSGSM
                     VGAYSMTDDQGRVFEAAIPAFSLDVPGGRTLN"
     gene            1614..>2234
                     /locus_tag="VF633_12710"
     CDS             1614..>2234
                     /locus_tag="VF633_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX8471966.1"
                     /translation="MTLDLTKVGILPAQSIETLIATGAITSQTAFDADQVQPASLDLR
                     LSDRAWRVRASFLPGQRLVADRIADVAMHAIDLSGGYVMEKGCVYIAELQERLSLPKG
                     LIARANPKSSTGRVDVFVRLLTDRGARFDDVAEGYDGPLYMEIAPQTFSILVRPGTRL
                     NQLRLKTPETPKLETRSVGVDLRGGDIVGFRGRRHAGVVDLENIDGH"
BASE COUNT          377 a          821 c          658 g          378 t
ORIGIN      
        1 ttcctcgatg ctttcgccgg gctccaggaa ccccgccagc gccgacatcc gccccgcagg
       61 ccaggccgcc tgacgcccca gcaggcagat cggctcgggc ccgcccttgt agaccggcag
      121 catgatggtg accggatcga cgcgcgggaa atgctcggta ccgcacgccg gacagacccg
      181 cttccagcca ccgttgccgt tgtcgctggc gttgccgcag gcggcgcaga agccgtgccg
      241 tcgccgccag tcgaacagcg acttggccgt ccccgccatg gccgcctcgg cgcccggcaa
      301 gactgcagcc gcctcccgca tttcggtgaa ccggcccagc ccgtgcacag ccgcgtccgc
      361 cggatcgacc gggctctcga actccacggc gaacaccggc gcgtctttcc acagtccgag
      421 gaacagctcg cgatccttca ccagatcgcg cgcatgcggc agcgacaccc aggccagcct
      481 cggaccctca ggcccgacct cgagcaacgg cttgccctcc cacagcacca tcgccagcgc
      541 gtcgaccttc tcagcctgct ccgccaacca cgacggatcg tttctcaggt cgccggctcg
      601 atcgagcgga ttgccggcga aggtgttcaa aaacggaagg gacatggacc gcacatagcc
      661 ctccagacgc ccgcgtggga gaggcggctt gatatcctcg tcgagattcg cgatatgggc
      721 ctcggagatc ggtgcgggcg aaggcttcgc caacctggtc agagccggaa ggcagcagcc
      781 acaacgaatt ccccttcggg tcgtgctggt ctccactctt ttgcttttag ggcgctacgc
      841 tcggctcgcg gagcctcgct acttgagcgc aaggctttct agcgctcgct gcccagcccc
      901 attccaattt ccgctcatcc ccgcgaaagc ggggacccag ttctggataa ggttgggatg
      961 gcaccacgca ccttgtttca ccggtcatga acccaaagca ctgggtcccc gcgttcgcgg
     1021 ggatgagcgg atgttgggga gcggacccgc cctaagccct aagcccgaac ccccaagccc
     1081 taaccccggc gccctgaacg ctagttcagc gtgcgccccc cgggcacatc cagactgaat
     1141 gccgggatcg cggcctcgaa cacccgcccc tgatcatccg tcatcgaata cgccccgacc
     1201 atcgagccgg acggcgtgcc cagcggacag cccgaggcat aggaatagct gtcgcccggc
     1261 gcgatcaccg gctgttcacc aatgacgccc ggaccctcga cgtgttcgac atggccgcgt
     1321 gcatcggtaa tgacccaccg ccgcgacacc agctggactg tgctcatgcc cagattgatg
     1381 atctcgacct gataggccca gacccaacgc cccgcctcgg gatcggactg ccccgccaga
     1441 tagctgggcc gcaccttgat cagaatgccg tcggtttcag cggtgtaggc gggcgtatcg
     1501 tgcattctgg aagggcctgc cgcgcttgcg ctacttgagg ccccactttc ctccgcgcgg
     1561 ctcatcgctt caacccctct tcctttttcg cttccccgtg tatggctgtc gcaatgaccc
     1621 tggaccttac caaagtcggc atcctgcccg cccaatccat cgagaccctg atcgccactg
     1681 gagcgatcac gtcgcagacc gcgttcgacg ccgatcaggt gcagcctgcc agtctcgacc
     1741 tgcgcctgtc cgaccgcgca tggcgtgtgc gcgcgtcctt cctgccgggc cagcgtctgg
     1801 tcgccgaccg gatcgccgac gtcgccatgc atgcgatcga cctgtcgggc ggctatgtga
     1861 tggaaaaggg ctgcgtctat atcgccgagc tgcaggagcg tctcagcctg cccaagggcc
     1921 tgatcgcccg cgcaaatccc aaatcctcga ccggccgtgt cgatgtcttc gtgcgcctgc
     1981 tgaccgatcg cggcgcccgg ttcgacgacg tggccgaggg ctatgacggg ccgctctata
     2041 tggagatcgc gccccagacc ttctccatac tggtccgtcc gggcacgcgc ctgaaccagt
     2101 tgcgcctcaa gacccccgag acgcccaagc tcgaaacccg cagcgtcggc gtcgatctgc
     2161 gcggcggtga catcgtcggc ttccgcgggc gtcgccatgc gggcgtcgtc gatctggaga
     2221 acatcgacgg ccac
//
LOCUS       DASVTM010000460         6519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_894052,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000460 DASVTM010000000
VERSION     DASVTM010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894052"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VF633_12715"
     CDS             <1..233
                     /locus_tag="VF633_12715"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX8471967.1"
                     /translation="GVPVQSKALSGLDSLLSIVQMPGGVPVATLAIGEAGAKNAGILA
                     AQILALSDDALAERLAAYRTAQTDAVAETVED"
     gene            241..1311
                     /locus_tag="VF633_12720"
     CDS             241..1311
                     /locus_tag="VF633_12720"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX8471968.1"
                     /translation="MPLPPGSTIGILGGGQLGRMLSQAASRLGFDVVILDPEDNSPAG
                     RVSRGQMVAAYDDPTALTVLGQVCDVVTFEFENVPAASVELLAEAGALVAPGPTALST
                     AQDRVDEKTFLNAVGATTVDFEPVDSVDDLIAGLERLGVPSLLKTRRDGYDGKGQIWI
                     RSITEAADAFAAIGERPAILEAKAAFTRELSVIAARGRDGQVAVYPLGENRHANGVLS
                     TTLAPAAIDATTAARAHEIAEAILDGLDYVGVMGVELFDLGDGVLLVNEIAPRVHNTG
                     HWTQDGCACDQFEQHIRAIAGWPLGPTKAHARVEMTNLLGDEIDQWTALAAEPNARLH
                     LYGKAEARPGRKMAHVNRVSKL"
     gene            complement(1308..1919)
                     /locus_tag="VF633_12725"
     CDS             complement(1308..1919)
                     /locus_tag="VF633_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX8471969.1"
                     /translation="MCASYESRFTVRQLVDAFSAAGTPFHSGVSLPNLAEVDEVRPTD
                     RSAVVMAGPRGEAIMASLSFGFPPPRPKARPVVNLRGEGRVFQNGDQTGRCLVPMTGF
                     YEFTGSTYPKTRWVFRDPEAPLLCLAAVWRAGESGAAESFALLTSEPGPDIAPYHDRA
                     VVIAPPKRWADWLAGDVFPADMIQPAPSGTVIVSAAPRPPKGD"
     gene            complement(1923..2597)
                     /locus_tag="VF633_12730"
     CDS             complement(1923..2597)
                     /locus_tag="VF633_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEX8471970.1"
                     /translation="MTEADHLPNEAPVPDWADRMEQTVLDAAIQRAPVFGWNSRLVRA
                     ACGAEGLSLGDQELLLPNGPRDLAALLSRRHDAKALETLKATPAQTLKMRERISRGVS
                     ARIEAGVDHLEAAKRCAAFLALPTNVDLGLSLARESADVLWRWAGDTATDWNHYSKRV
                     ILSGILIPALTMRLFDGKEAADRFVAARIENVMAFEKWKAGKDFEAPLKKVTDALARL
                     RYGAPT"
     gene            2808..3077
                     /gene="rpsU"
                     /locus_tag="VF633_12735"
     CDS             2808..3077
                     /gene="rpsU"
                     /locus_tag="VF633_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX8471971.1"
                     /translation="MVQIFVRDNNVDQALKALKKKMQREGSFREMKRHVHYEKPSEKR
                     ARQKAEAIRRARKLARKRMQREGLLPPPKPRVPGPGGAAGGNNRS"
     gene            complement(3308..4699)
                     /locus_tag="VF633_12740"
     CDS             complement(3308..4699)
                     /locus_tag="VF633_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8471972.1"
                     /translation="MKRTLLIARREFLAYARTVGFWLSLLSLPFFALLGGGIPLLVRS
                     SEPVREVVLVEEGPQAVGLASAVRDVIASDAERRRQRAAEAAEKAAAVPGGAANSATA
                     RNAVASLTKDRVRIISTPTALASAQPGPTQDAVVRRLLSEDTPEAQRPDAVIFLTRDD
                     GKPAARVWTAKASDSTIEDFTRDALRTANRRQVFQNAGIDLSVVVGTERFRPVVKAFS
                     PRSVSGAEVSLRDRLPGYVGLAAAFLLWSLVITGASMLLNSVMEEKSNKILEVLLSSA
                     SATEILAGKVLGVAMLTLTVLIVWSGLGAFGLIGAAPGVAADVGAVLLHNGLVFYFFA
                     YMVGGYLMYAVLFAAIGAFCETPRDAQSLMGPIMMVLVVPLLVLQLAIQNPDAPLVRI
                     LSWVPFFTPFLMSARVPSEPPMIEIIGTLAGMFITALLMVWLAGRAFRAGALSDVKLS
                     WKSFANAMRGSGN"
     gene            complement(4696..5661)
                     /locus_tag="VF633_12745"
     CDS             complement(4696..5661)
                     /locus_tag="VF633_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8471973.1"
                     /translation="MVAALTLDGIGKRYGDFQAVRDLSFQVEKGTICGFLGPNGAGKT
                     STIRMILGLQAATAGRIEILGADDGRKVRDRIGFLPEERGLYKKMTPVDAIAFFASLK
                     GVPTAEGRRRARDMLDAQGLGASKNKKMKEMSKGMAQKVQLIASVVHRPEFVILDEPF
                     SGLDPVNQQGLESVIRGLAADGATVLFSTHVMQHAERLCDKVVLLARGRKAFEGTVTE
                     ARATSRRFLELEGDIPAEAVCRLPGVTGVEIAAEGEGSRTLRAALATGAGGQETLKAA
                     FLSGLDVRRFELREPSLHDAFIDLTGEDPNRDQSVAKASVMEAAR"
     gene            complement(5759..6376)
                     /locus_tag="VF633_12750"
     CDS             complement(5759..6376)
                     /locus_tag="VF633_12750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8471974.1"
                     /translation="MRLYAPRLRALLIHTSQTAGLVAIACLAMAAAPISSRVTAEAAP
                     EVSVPVKPPVVAVAAPPKPMMRPMIFAEPVRGHAINSKFGMRRLGGEPGARQHKGVDI
                     AAPTGTSVYGSAEGVVLRIGYDPAGYGNFIEMRHPNGMTSLYGHLSRVDVASGDKVTS
                     GQRIGLIGSTGYSTGPHLHFEIRREGAQINPAKVIGRSFQVKAGR"
BASE COUNT         1154 a         2264 c         2067 g         1034 t
ORIGIN      
        1 tcggcgtgcc ggtccagtcc aaggcgctga gcggcctcga ttcgctgttg tctatcgtcc
       61 agatgcccgg cggcgtcccc gtcgcaacct tggccatcgg cgaagcgggc gccaagaacg
      121 caggcatcct cgccgcccag atcctcgccc tgtccgacga cgccctcgcc gagcgactcg
      181 ccgcctatcg caccgcccag accgacgcgg tcgcggaaac ggtcgaggac tgagttgagc
      241 ctgcctctcc ctcccggttc gaccatcggc atcctcggcg gcggccagct cggccggatg
      301 ctgtcgcaag ccgcctcccg gctcggcttc gacgtcgtca tcctcgatcc agaagacaac
      361 agccccgccg gtcgagtctc acggggccag atggttgccg cctatgacga cccgaccgca
      421 ctgacggtgc tgggtcaggt ctgcgacgtg gtcaccttcg agttcgagaa cgtgcccgcc
      481 gcctcggtcg aactgctggc ggaagccggg gctctcgtcg cgcccgggcc gaccgcgctg
      541 tcgacggctc aggaccgggt cgacgagaag accttcctca acgccgtcgg cgcgaccacg
      601 gtcgatttcg agccggtcga cagtgtcgac gacctgatcg ccggactcga gcggctgggc
      661 gtcccgtcgc tgctcaagac ccgccgcgac ggttatgacg gcaagggcca gatctggatc
      721 cgctcgatca ccgaggccgc cgacgccttc gccgccatcg gcgaacgccc tgccatcctc
      781 gaggcgaagg ccgccttcac gcgcgaacta tcggtcatcg cagcgcgtgg tcgtgacggt
      841 caggtcgccg tctatccgtt gggcgagaac cgccatgcca acggcgtcct cagcaccacg
      901 ctcgctcctg ctgcgatcga tgcgaccacg gccgcacgtg cgcacgagat cgccgaagcc
      961 atcctcgacg gcctcgacta tgtcggcgtc atgggtgtcg aactgttcga cctcggtgac
     1021 ggcgtcctgc tggtcaatga gatcgcgccg cgcgtccaca acaccggcca ctggacccag
     1081 gacggctgcg cctgtgatca gttcgaacag cacatccggg ccattgccgg ctggccccta
     1141 ggccccacca aggcccacgc ccgcgtagaa atgaccaacc tgctcggcga tgagatcgac
     1201 cagtggacgg cgttagccgc cgaacccaac gcccgcctcc acctctacgg caaggccgaa
     1261 gcccgcccgg gccggaaaat ggcccacgtg aaccgggtgt cgaagctcta gtcgcccttc
     1321 ggcggtctcg gcgccgcgct gacgatcaca gttcccgatg gcgcgggctg aatcatgtcc
     1381 gccggaaaga catcgccggc gagccagtcg gcccatcgct tgggtggggc gatgacaacg
     1441 gcccggtcat gatagggcgc gatatccgga cccggctcac tggtcaacag cgcgaacgac
     1501 tccgctgctc ccgactcgcc cgcgcgccag acggccgcca ggcacagaag cggcgcctcg
     1561 ggatcgcgaa acacccagcg ggttttcggg taggtgctcc cggtgaactc atagaagccc
     1621 gtcatcggaa caaggcagcg tccggtctgg tccccgttct ggaacacccg accctcgccc
     1681 ctcagattga caacgggcct ggctttggga cgcggcggtg gaaagccgaa ggacagggag
     1741 gccatgatcg cctctcccct cggccccgcc atcacaacgg cggagcgatc cgtcggacgc
     1801 acctcgtcga cctcggcgag attgggaagg cttacgccgc tgtgaaacgg tgttccggcc
     1861 gcagaaaagg cgtctaccag ttggcggacg gtgaaccggc tttcatagga agcgcacatc
     1921 gctcaggtcg gggctccata tcgcagcctc gccagcgcgt cggtgacctt cttcagcggg
     1981 gcctcgaagt ccttgccggc cttccacttc tcgaaggcca tgacgttctc gatgcgggcg
     2041 gcgacgaatc tgtccgccgc ctccttgccg tcgaacaggc gcatcgtcag ggctgggatc
     2101 aggatgcccg acaggatcac gcgcttggaa tagtggttcc agtccgtcgc cgtatccccc
     2161 gcccatcgcc acagaacgtc ggcgctctcc cgcgccagcg acaggccgag atcgacgttg
     2221 gtcggcaggg ccagaaaggc cgcacaccgc tttgccgcct caagatggtc cacgcccgcc
     2281 tcgattcgcg ccgagacccc tcgtgagatt cgctcacgca tcttcagcgt ctgcgccggt
     2341 gtcgccttca gcgtctccag cgccttggcg tcatgccgac gcgacagcag cgcagcgaga
     2401 tcgcgcgggc cgttgggcag caacagttcc tgatcgccga gcgacagccc ctcggccccg
     2461 cacgccgccc gcaccagccg tgaattccag ccaaacaccg gcgcacgctg gatcgcggcg
     2521 tccagcaccg tctgttccat gcgatcggcc caatctggaa ctggcgcttc gttcggcagg
     2581 tggtcggctt cggtcatggc catagaatag gcttgtccgg cctctcgcgc gaccccatct
     2641 gtcagctctt gagagcccgg tgattgctcg ccaccgaact ggacagacag cggctcgtgg
     2701 tgtattcgcc cgccttcgct cgaagggctc tcgcccccgg gctgctattt tatttgtctg
     2761 cccgtctccc tcacaggacg cggcgggcgg ccaaaggaga gttcaacctg gtacagattt
     2821 tcgttcgcga taataacgtc gatcaagcgc tcaaagccct aaagaagaag atgcaacgcg
     2881 aaggcagctt ccgtgaaatg aagcgccacg tgcactacga gaagccctcg gagaagcgcg
     2941 cccgtcagaa ggctgaggcg atccgtcgcg ctcgcaagct ggcccgcaag cgcatgcaac
     3001 gcgaaggcct gctgccccct ccgaagcccc gcgtgccggg tccgggcggt gctgccggcg
     3061 gcaacaatcg ctcttaagcg actgttccaa aacgagattt gaaaaggccg ctccctcagg
     3121 ggagcggcct ttttgttatc tggcttgcct tctccccttg cgggagaagg tggcaggcga
     3181 agcctgacgg atgaggggtc acgcaggcct atagagtgag cgatggccaa ccttagatgt
     3241 gggcacgacc cctcatccga gccgccctgc ggcccacctt ctcccgcagg gggagaagga
     3301 cagggactta gttcccactg ccccgcatgg cgttagcgaa gctcttccag ctcagcttca
     3361 cgtcggacag cgccccggcg cgaaacgccc ggcccgccag ccagaccatc agcagcgccg
     3421 tgatgaacat ccccgccagc gtgccgatga tctcgatcat cggcggctcc gaaggcacgc
     3481 gagcgctcat caggaacggc gtgaagaacg gcacccacga caggatccga acaagtggag
     3541 cgtccgggtt ctggattgcc agctggagca ccagcagcgg caccaccagg accatcatga
     3601 tcgggcccat caaggactgg gcgtcgcgag gtgtttcgca gaaggctccg atggctgcga
     3661 acagcactgc gtacatcagg taaccgccga ccatataggc gaagaagtag aagacgaggc
     3721 cgttgtgcag cagcaccgcc cccacatccg ccgccacacc cggcgctgcg ccgatcaagc
     3781 cgaacgcgcc gagaccgctc cagacgatca acaccgtgag ggtcagcatc gccacaccca
     3841 ggaccttgcc ggccaggatc tcggtcgccg aggccgagga cagcaggacc tccaggatct
     3901 tgttcgactt ctcctccatc acgctgttca ggagcatgga tgccccggtg atgaccagcg
     3961 accacaacag gaaggccgcg gccagcccga catagcccgg cagccggtcg cgcaacgaca
     4021 cctctgcgcc gctgaccgag cgaggcgaga aggcctttac cactggcctg aaccgctcag
     4081 tcccaaccac gacggacagg tcgatgccgg cgttctggaa cacctgacgg cggttggcgg
     4141 tgcgcagggc gtcgcgggtg aagtcctcga tggtcgagtc ggacgccttg gcggtccaca
     4201 cccgggcggc gggcttgccg tcatcccggg tcaggaagat tacggcgtca gggcgctggg
     4261 cctcgggcgt atcctcgctc agcaggcggc gaacgacggc gtcctgtgtc gggccgggct
     4321 gggcactggc aagagcggtg ggcgtcgaga tgatccgcac ccgatccttg gtcagggagg
     4381 cgacggcgtt gcgggctgtc gcactgttgg cggcgccgcc gggaaccgca gcggctttct
     4441 cggcggcctc ggcggcacgc tgtcggcggc gttccgcgtc ggaggcgatg acgtcacgaa
     4501 cggcggaagc caaacccacg gcctgcggac cttcctcgac cagcacgacc tcgcgcaccg
     4561 gttccgacga gcggaccagc aagggtatgc caccgccgag aagcgcgaag aacggcaggg
     4621 acagcaacga cagccagaag ccgacggtgc gggcataggc caggaactcg cggcgggcga
     4681 tcagcagggt gcgtttcatc gggcggcctc catcacagaa gctttcgcca cggactggtc
     4741 ccggttggga tcttcgcccg tcaggtcgat gaaggcgtca tgcaagctgg gctcgcggag
     4801 ctcgaaccgg cgtacgtcca gccccgacag gaaggccgcc ttaagcgtct cctgccctcc
     4861 tgcccccgtc gccagggcgg cgcgtagtgt gcgtgagccc tcgccctcgg ctgcgatctc
     4921 gaccccggtc actccgggca gtctacatac ggcttcggcg ggaatgtcgc cctccagttc
     4981 gaggaaccgc cgtgacgtgg cgcgcgcctc ggtgaccgtg ccctcgaacg ccttgcggcc
     5041 ccgtgccagc agcacgacct tgtcgcacag ccgctcggca tgctgcatca catgggtcga
     5101 gaacagcacc gtcgcgccgt cggcggccaa tccccggatc acgctctcca gcccctgctg
     5161 attgaccgga tccagccccg agaacggctc gtccaggatc acgaactcgg gccgatgcac
     5221 gaccgaggcg atcaactgaa ccttctgggc catccccttg gacatctcct tcatcttctt
     5281 gttcttcgac gcgccgaggc cttgcgcgtc cagcatgtcc ctggcccggc gacgtccctc
     5341 agccgtgggt acgcccttca acgaagcgaa gaaggcgatg gcgtccacgg gggtcatctt
     5401 cttgtaaagg ccgcgctcct ccggcaggaa gccgatccga tcgcggacct tgcggccatc
     5461 gtcggcgccg aggatctcga tccgcccggc cgtcgccgcc tgcaaaccaa ggatcatgcg
     5521 gatggtcgag gtcttgcccg cgccgttggg cccgaggaag ccgcagatcg tgcccttctc
     5581 aacctgaaag ctcagatcac gaacggcctg gaagtcgccg tagcgcttgc cgatcccgtc
     5641 cagcgtcaac gccgcaacca tacgtcatat ctcccctgag gaagcagacg tatcaaacgc
     5701 cgtgggcatg gcaaccggat tcggcgatgc cctgtggagc gtggcgttca gcccgccctt
     5761 accggcccgc cttgacctgg aagctgcgcc cgatgacctt ggccggattg atctgggcac
     5821 cctcgcgacg aatctcgaaa tgcaggtgcg ggccggtcga atagcccgtc gagccgatca
     5881 ggccgatccg ttgaccactc gtcaccttgt caccgctggc gacgtccacc cggctcagat
     5941 gcccgtacag gctggtcatg ccgttaggat gacgcatctc gatgaagttg ccatagcccg
     6001 ccgggtcata cccgatgcgc aagaccacgc cctcggcgga tccatagacc gaggttcccg
     6061 tcggcgcggc gatatccaca cccttgtgct gacgcgcgcc gggctcgcca ccgagacgac
     6121 gcatgccaaa cttcgagttg atggcgtggc cccgcaccgg ctctgcgaag atcatcgggc
     6181 gcatcatcgg tttaggcggc gcggcgacgg cgacaaccgg cggcttcacc gggaccgaga
     6241 cctcaggagc ggcctcggcg gtcactctcg aggagatagg agcggcggcc atcgccagac
     6301 aagcgatcgc aaccaggcct gcggtctggc tggtgtggat caggagggca cgcaggcggg
     6361 gcgcatacag gcgcatatgt cgttcggctc cggcgtcgct gctatgagcg ggagacggcc
     6421 gcgcccaccg accgacggtg tcggaacggc ctatggcgat gaccgggggg agccggtgct
     6481 tctaagactc catggggcga ctttggggcg ctcaggagg
//
LOCUS       DASVTM010000461         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_791063,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000461 DASVTM010000000
VERSION     DASVTM010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791063"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..655
                     /locus_tag="VF633_12755"
     CDS             86..655
                     /locus_tag="VF633_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEX8471975.1"
                     /translation="MTPGAIAILSLGMSTDAFAAAVGRGASHRLKLVEAAKAGLVFGV
                     IEGITPLIGWGLGILAAGFVEQVDHWIAFGLLGAVGGKMIWEALKARDDEEDESPSRS
                     GPWVLIATAVGTSIDAAAVGVGLAFIGVNIWIIAASIGFTTFVCTTIGMMIGSYVGRR
                     FGKIAELIGGIALIALGCGILFEHLGIIH"
     gene            complement(684..1442)
                     /locus_tag="VF633_12760"
     CDS             complement(684..1442)
                     /locus_tag="VF633_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX8471976.1"
                     /translation="MAHTTSAEKAPRLAVLIDAENASSRIAEALFTEIATLGEASARR
                     IYGDFASPQLKGWTDVLARFAIQPQQNFASTKGKNSGDIALVIDGMDLLHSDRFDGFC
                     LVSSDSDFTRLASRIREEGVDVFGFGEQKTPESFRQACTRFIYTENLPIAASGAETPK
                     SGQTPVLPPAEASVRPKPSQAIPLIRAAIADMDEDGDGWVAVGGIGSRLRNAHPDFDQ
                     RTYGHVKLSDLIRATGRYEVESRPAGGLWMRKKA"
     gene            complement(1487..1729)
                     /locus_tag="VF633_12765"
     CDS             complement(1487..1729)
                     /locus_tag="VF633_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471977.1"
                     /translation="MATETVYIVQAYVQGRGKALKAEQQVGCKDAEEARRKAERLAPL
                     RLGVVAFSVSADTEMGDYDESPTIIFKSGQLPEMFD"
     gene            1795..2202
                     /locus_tag="VF633_12770"
     CDS             1795..2202
                     /locus_tag="VF633_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471978.1"
                     /translation="MLRDMPGVDDLEMKALMKPRFADPDEPEGRDEIDALSLAIFGLS
                     LDAADEVEKPADWDRIERLSPSDMVEAFEAEGWDVTDKKRKPLRLLPHLALPLALAIR
                     GVAGELPFFAEPDVKETDWGSNLAAEASRFRKR"
     gene            complement(2203..>2642)
                     /locus_tag="VF633_12775"
     CDS             complement(2203..>2642)
                     /locus_tag="VF633_12775"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167794.1"
                     /note="catalyzes the formation of pyruvate and glyoxylate
                     from 4-hydroxy-2-oxoglutarate; or pyruvate and
                     D-glyceraldehyde 3-phosphate from
                     2-dehydro-3-deoxy-D-glyconate 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX8471979.1"
                     /translation="APTAALYMSRGARFVVSPCLVPEVARVCNRHMVPYFPGCGSVTD
                     IGEAQELGCEIVKLFPGSSVGGADFVKAVLGPMPWTKIMPTGGVDPDEASIAKWFGAG
                     IVAAGMGSKLITDAAIKAKDWAGIEANVKMAVDNIAAFRASKA"
BASE COUNT          444 a          793 c          904 g          501 t
ORIGIN      
        1 gacgggcgcc tagcgagacc agcgtttcca acgccgttcc gggggcgtgg ggaagcgttg
       61 ctcgtcctcc cggcggacca tgatcatgac tcctggcgct atcgccatcc tctcgctcgg
      121 catgtccacc gacgccttcg ccgctgccgt cgggcgcggg gcgtcccaca ggctcaaatt
      181 agtcgaggcc gccaaggccg gtctggtgtt cggggtgatc gagggcatca cgccactgat
      241 cggctggggc ctcgggattc tcgcggcggg gtttgtcgag caggtggatc actggatcgc
      301 cttcggcctt ctgggcgccg tgggcggcaa gatgatctgg gaggcgctga aggccagaga
      361 cgacgaggaa gacgaatccc ctagccgttc cgggccgtgg gtgctgatcg cgacggccgt
      421 cggaaccagc atcgatgccg cggcggtggg cgtggggctg gcgttcatcg gcgtgaacat
      481 ctggatcatc gcagcctcga tcggcttcac caccttcgtt tgcacgacaa tcgggatgat
      541 gatcggcagc tatgtcgggc ggcgctttgg caagatcgcc gaactgatcg gcggcatcgc
      601 cctgatcgcc ctgggctgcg gcatcctgtt cgaacacctc ggcatcattc actgatcggg
      661 aggccaagtt tctggccttt gtgtcaggcc ttcttacgca tccacaagcc gccggcaggt
      721 cgtgactcga cctcgtagcg gcctgtcgcg cgaatgaggt ccgagagttt aacgtgccca
      781 taggttcgtt ggtcaaaatc ggggtgggcg ttgcgcagcc ggctgcctat gccgcccacg
      841 gcaacccagc cgtcgccgtc ttcatccatg tcggcgatcg ccgccctgat cagcggaata
      901 gcctgtgatg gcttaggtcg caccgaagcc tccgccggag ggagaacagg ggtttgccct
      961 gattttggtg tctcggcgcc cgacgccgcg attggcaggt tctctgtgta gatgaagcgg
     1021 gtgcaggctt ggcggaagct ttcgggtgtt ttctgctcgc caaagccgaa gacatctacg
     1081 ccttcctcgc ggatacgcga ggctagcctg gtgaagtcgc tgtccgatga cacgaggcag
     1141 aagccgtcga accggtccga atgcagcaga tccatgccgt cgatgacgag ggcgatgtcg
     1201 ccggagttct tgcctttggt gctggcgaaa ttttgctgcg gctgaatggc gaaccgcgcg
     1261 aggacatcag tccagccctt gagttgcggg ctggcgaaat cgccgtagat gcgccgcgcg
     1321 ctggcctcgc caagggtggc gatctccgtg aacaaggcct cggcgatgcg ggacgacgca
     1381 ttctcggcgt cgatcagaac cgccagacgc ggcgccttct cggctgaggt ggtatgggcc
     1441 atggctgttc tcctgaaacg tcctcgatca ggccagcaac tctggatcag tcaaacatct
     1501 cgggcaactg gccggacttg aagatgatgg tggggctttc gtcgtagtcg cccatctcgg
     1561 tgtcggcgga gacggagaag gccacgacgc caaggcgtaa gggggcgagg cgttcggcct
     1621 tgcggcgggc ctcctcggca tccttgcagc cgacctgctg ctcggccttg agggccttgc
     1681 cgcgtccctg gacgtaggcc tggacgatat agacggtttc agtggccata gtgctgcatg
     1741 gcggcctgtt cgcccggacg gtcaagcgct aaaccgtccg gcatgaccct tagaatgctc
     1801 agagatatgc ccggcgtcga cgatctggag atgaaggcgc tgatgaaacc gcgcttcgcc
     1861 gatccagacg agcccgaggg ccgcgacgag atcgacgcct tgtcgctcgc cattttcggc
     1921 ctgtcgttgg atgcggcgga cgaggttgaa aaacctgccg actgggaccg gatcgaacgg
     1981 ctgtcgccct cggatatggt cgaggcgttc gaggctgagg gctgggatgt caccgacaag
     2041 aagcgcaaac cattgaggct gttgccgcat ctggccctgc cgctggccct ggccattcgc
     2101 ggggtggcgg gcgagttgcc gttcttcgca gaacccgacg tcaaggaaac cgactggggc
     2161 tccaatcttg cggcggaggc cagtcggttc cggaaacgct gatcaggctt tggaggcccg
     2221 gaacgccgcg atgttgtcga cggccatctt cacattggct tcgatgcccg cccagtcctt
     2281 agccttgata gcggcgtcgg tgatcagttt ggagcccatg cccgccgcga cgatgccggc
     2341 gccgaaccat ttggcgatcg aggcttcgtc gggatcgacg ccgccggtcg gcatgatctt
     2401 ggtccagggc atggggccga gcacggcctt gacgaagtcc gcgccgccga cggacgagcc
     2461 ggggaacagt ttgacgatct cgcagcccag ttcctgagcc tcgccgatgt ccgtgaccga
     2521 gccgcagccc gggaaatatg ggaccatgtg gcggttgcag acgcgggcga cctcggggac
     2581 gaggcagggc gaaaccacga agcgggcgcc gcgcgacata tacagggctg cggtcggggc
     2641 at
//
LOCUS       DASVTM010000462         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_736943,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000462 DASVTM010000000
VERSION     DASVTM010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736943"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VF633_12780"
     CDS             <1..968
                     /locus_tag="VF633_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX8471980.1"
                     /translation="LKIEAMMDAGLEEGLAPPSRLGRVKRIDDAPPRYIEIAKSTFPR
                     GLSLKGLRVAIDCANGAGYKVAPTTLYELGAEVFSVGIDPNGTNINADCGSTHPGTLA
                     EAVKTYRADIGIALDGDADRLIVCDEKGTIVDGDQLMALIGLDAARQGTLTGGGIVAT
                     VMSNLGLERKMKSEGLTLERTKVGDRYVMERMREGGFNIGGEQSGHIILHDHATTGDG
                     LLAALQVLAVMVQTGKPMSELARQFEGVPQLLENVRYTGGKPLENAHVISAIADAEHT
                     LNGSGRLLVRPSGTEKLIRVMAEGDDEAMVKRVVSDVSAAVRAAG"
     gene            complement(969..1814)
                     /locus_tag="VF633_12785"
     CDS             complement(969..1814)
                     /locus_tag="VF633_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471981.1"
                     /translation="MIGITRTASLALIGSLVLASSVAAQTEGPNTPAPATQAEKPRTQ
                     VNIGRAEPQGSEGRDLRLLNTAMGAVQRSGFSGLRRHVRALKQALDHAPATYPLVEQT
                     PARWIVRSDDFSQVMMVGLMASASGAPSIDVVQQSNVYGTIALVLTSEAVERHAYAEA
                     IGYADQGLALQPQNAMLVSEKASALLGLGRFDEMLATVDAALPDVGLLSGTDRALLQR
                     RRGSALIELGRLDEAKRAFEDSLVSEPGNAAAANELQYIEGLKAGRPGGPLTLTTVPR
                     STTDQ"
     gene            complement(1892..2149)
                     /locus_tag="VF633_12790"
     CDS             complement(1892..2149)
                     /locus_tag="VF633_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8471982.1"
                     /translation="MMTAIFVFIKCELGHANDVAADMVDNVENVSEVYSTSGQYDLLA
                     KFQLPKEMDIGTFVTKSVQTRPHIRDTFTVITFSPFLPSGK"
     gene            2214..3002
                     /locus_tag="VF633_12795"
     CDS             2214..3002
                     /locus_tag="VF633_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8471983.1"
                     /translation="MDLEAEYNNRAAVPDHPMVMARWKADAEAARAAHPPVEIAYGPG
                     EREVMDVFDAGPEAPVVVFIHGGYWQALDKSWFSWIAPALLSHGVSVAIPSYDLCPTV
                     RLGRIVEQMRAVTILLRERTGKRPVVTGHSAGGHLSACMLSEGKASGAVAISGVFDLR
                     PLIETSINTALGLYANEAAALSPIFWPAPNGGAPGGTELDCIVGAETSEFIRQSRDMA
                     DVWSAKGADTRFEALDGLNHVTILDPLADPDSGLVKRIVEMAKQ"
     gene            3103..>3358
                     /locus_tag="VF633_12800"
     CDS             3103..>3358
                     /locus_tag="VF633_12800"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259465.1"
                     /note="catalyzes the formation of pyruvate and glyoxylate
                     from 4-hydroxy-2-oxoglutarate; or pyruvate and
                     D-glyceraldehyde 3-phosphate from
                     2-dehydro-3-deoxy-D-glyconate 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX8471984.1"
                     /translation="MRHDRLSVLIALEKQGVAPVFYHPDPEVCLNIIRACARGGAPAV
                     EFTNRGDFAADLFGEIHRELIHTDPHIVLGIGSIVDAPTAA"
BASE COUNT          608 a         1139 c         1034 g          577 t
ORIGIN      
        1 agctcaagat cgaggccatg atggacgccg gtctcgaaga gggtctggcc ccgcccagcc
       61 gcctcggccg ggtcaagcgc atcgacgacg caccgccccg ctatatcgaa atcgccaaat
      121 ccaccttccc gcgtggcctc agcctcaagg gcctgcgggt cgccatcgat tgcgccaacg
      181 gcgccggcta caaggtcgcc ccgacgacgc tttacgaact gggcgccgag gtcttttccg
      241 tcggcatcga tcccaacggc accaacatca acgccgattg cggctcgacc catccgggca
      301 cactggccga ggcggtcaaa acctaccgcg ccgacatcgg catcgccctg gacggtgacg
      361 ccgaccggct gatcgtctgc gatgagaagg ggacgatcgt cgacggcgac caactcatgg
      421 ctctgatcgg tctcgacgcc gcgcgtcagg gcacgttgac cggaggcggc atcgtcgcca
      481 ccgtcatgtc caacctcggc ctcgagcgaa agatgaagtc cgagggcctc acactggagc
      541 gcaccaaggt cggcgaccgc tacgtcatgg agcggatgcg cgagggcggc ttcaacatcg
      601 gcggcgaaca gtccggccac atcatcctgc acgatcacgc caccaccggc gacggcctgt
      661 tggcggcgct gcaggtgctg gcggtgatgg tccagaccgg caagccgatg agcgagctgg
      721 ctcgccagtt cgagggcgtg ccacagctgc tggaaaacgt ccgctacacc ggcggcaagc
      781 cgctcgaaaa cgcccacgtc atctccgcca tcgccgacgc cgaacacacg ctgaacggct
      841 ccggccgctt gctggtccgc ccgtccggca ccgaaaaact gatccgcgtc atggccgaag
      901 gcgacgacga agccatggtg aagcgcgtgg tgtcagacgt gtctgcagcg gtgcgggcgg
      961 cggggtagtt actggtcggt cgttgaccga gggacagtcg taagcgtcag aggcccacca
     1021 ggtcgccccg ctttaagccc ctcaatgtac tgcagttcat tagcggctgc ggcgttaccg
     1081 ggctccgaca ccagcgagtc ttcaaaggcc ctcttcgctt cgtccaaccg gcctagttcg
     1141 atcaaggccg aaccccggcg acgttgtagc aacgccctat cggtaccaga caacaagccg
     1201 acgtctggca gcgccgcatc gaccgttgcg agcatctcgt caaaccgccc cagacccaac
     1261 agagccgagg ccttttcgga gaccagcatc gcgttctgtg gctgcagcgc aagcccttgg
     1321 tccgcatagc ctatggcctc cgcataggcg tggcgctcca ccgcttccga cgtcagcacc
     1381 aaagcgatcg ttccatagac gttggactgc tggacgacgt cgatagacgg tgctccagag
     1441 gcgctggcca tcagtccgac catcatgacc tggctgaaat cgtcagagcg gacgatccag
     1501 cgtgccggag tctgttcgac tagcggatag gtcgccggcg cgtgatcaag cgcttgcttc
     1561 agggctcgaa catgcctgcg cagtcctgaa aatccgctgc gctggaccgc ccccatcgcc
     1621 gtgttaagga ggcggagatc tcgtccctcg cttccctgcg gttccgcacg accgatattc
     1681 acctgggtgc ggggcttttc ggcttgggta gcgggcgctg gcgtgtttgg gccttcagtc
     1741 tgcgcggcta cgctgctcgc cagcaccaga ctcccaatca gggcgagact cgccgtcctt
     1801 gtgattccga tcattcgcac aactcccgcc tgttcttgaa cagaaagcac gccttggcat
     1861 gtcagaccag atggcgggta taatgctgct cctacttccc gctcggcagg aacggcgaga
     1921 acgtgatcac cgtgaaggtg tcgcggatgt gcggtcgggt ctggacggac ttggtgacga
     1981 aggtgccgat gtccatttcc ttcggcagct ggaacttggc cagaaggtcg tactgacccg
     2041 aggtcgaata gacctccgag acgttctcga cattgtcgac catgtcggcg gccacgtcgt
     2101 tggcgtggcc cagctcgcat ttgatgaaga cgaagatggc ggtcatcatg gcggtcggct
     2161 ccgggacggc tttggggtcg gcttggtgtg cgaaacgtcg tagcgatcat ccaatggacc
     2221 tggaagccga atacaacaac cgcgccgccg tgccggacca cccgatggtc atggcccgct
     2281 ggaaggccga tgccgaggcg gcccgcgccg cccatccgcc cgtcgagatc gcctacggcc
     2341 ccggcgaacg tgaagtcatg gatgtcttcg acgcggggcc tgaagccccc gtcgtcgtct
     2401 tcattcacgg cggttattgg caggctttgg acaagagctg gttcagctgg atcgccccag
     2461 cgctgctgag ccacggcgtc agcgtcgcca tcccgagcta tgacctctgt ccgactgtcc
     2521 ggctcggcag gatcgtcgaa cagatgcgcg ccgtcacgat cctgctgcgc gaacgcacag
     2581 gcaagcgccc cgtggtgacc ggccattccg ccggcggcca cctgtcggcc tgtatgctca
     2641 gcgagggcaa ggcgtcggga gccgtcgcca tttccggcgt gttcgacctg cgtccgctga
     2701 tcgagacctc gatcaacacg gcgcttggcc tctatgcgaa cgaggccgca gccctgtcgc
     2761 cgatcttctg gcccgcgccc aacggcggcg cgcccggcgg caccgaactg gactgcatcg
     2821 tcggcgccga gacttcagag ttcatccgtc agagccgcga catggccgac gtctggtccg
     2881 ccaaaggcgc cgacactcgg ttcgaggcgc tggacggcct caaccatgtc acgatcctcg
     2941 accccctcgc cgatccggac agcggtttgg tgaaacggat cgtggagatg gcgaaacaat
     3001 aagttccgca ctgaaccgcg ccaaagcttt cccgctgacc accggttgcg ataggacacc
     3061 ccgaacaccc ggcgccggcc ggaccgagga gctttgaaga ccatgcgcca cgatcgcctc
     3121 agcgtcctga tcgctcttga gaagcagggc gtcgcccccg tcttctatca ccccgacccc
     3181 gaggtctgcc tcaacatcat ccgcgcctgc gcccgtggcg gcgcgccggc ggtcgagttc
     3241 acgaaccggg gcgatttcgc cgctgacctg ttcggcgaga tccatcgcga actgatccac
     3301 accgacccgc acatcgtgct cggcatcggc tccatcgtcg atgcgccgac cgccgcaa
//
LOCUS       DASVTM010000463         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_257037,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000463 DASVTM010000000
VERSION     DASVTM010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257037"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..>2076
                     /locus_tag="VF633_12805"
     CDS             25..>2076
                     /locus_tag="VF633_12805"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX8471985.1"
                     /translation="MTDLTLVVAEPPSATDLPPPPRVAATVGLPLDEALLQSPSRFFN
                     RELSWLAFNQRVLEEAENPRHPLLERLRFLSISANNLDEFFMVRVAGLKAQVREGVRV
                     ISQDGLTPSEQLAQVNTAASDLMAIQQARWTELRTELLQEGIGLCDPADVTPEERAVL
                     QAAFLSQLFPILTPLAIDPAHPFPFIPNLGFSLALKLTRQSDGRTLYALVPVPTQVQR
                     FWPLPFEEGERSRRYIALESLLALFTDHLFPGMQVEARGAFRIIRDSDVEIEEEAEDL
                     VREFEALLKQRRLGSVVRIKIEAAMPADLRTFIIANLRAEPQDISLVEGMLGLAQLSQ
                     LIPPDRQDLKFPPYEPRFPERVRDHGGVCFAAIREKDILVHHPFESFDVVVQFLRQAA
                     TDPDVLAIKQTLYRTSKDSPIVAALIEAAENGKNVTALIEIKARFDEEANLKWARDLE
                     RAGVHVVFGFIEYKTHAKLSLVVRREGESLTTYCHFGTGNYHPQTARVYTDLSLFTTH
                     APLGRDSARLFNFITGYALPEALEGLAFSPATLKSTLLTRIDREVENAGKGKPAAIWA
                     KLNSLVDPVIIDALYRASQAGVRIELVIRGICCLRPQVPGLSENIRVKSIVGRFLEHS
                     RIVAFADGRRMPSHRARLYISSADWMPRNLDRRVESLVPVINPTVHRQILDQIMVANL
                     ND"
BASE COUNT          342 a          738 c          651 g          345 t
ORIGIN      
        1 caccgaagcc ctaaatccta gcgcatgacc gacctgaccc tcgtggtggc ggagcccccg
       61 tcggcgaccg acctgcctcc gccaccgcgc gtggccgcca ctgtcggcct tccgctcgac
      121 gaggccctgc tgcagtcgcc ctcgcgcttc ttcaatcgtg aactcagctg gctggccttc
      181 aaccagcgtg tgctggaaga agctgagaac ccgcggcatc cactgcttga gcgcctgcgc
      241 ttcctgtcca tctccgccaa caacctggac gaattcttca tggtgcgcgt cgcgggcctg
      301 aaggcgcagg tgcgcgaggg cgtccgggtc atcagccagg acggcctgac gcccagcgag
      361 cagctggcgc aggtgaacac ggccgcatcc gatttgatgg ccatccagca ggcccgctgg
      421 accgagctcc gcaccgagct cctgcaagag gggatcggcc tatgcgaccc cgccgacgtc
      481 acgccggagg agcgggcggt gctccaggcg gcgttcctgt ctcagctgtt cccgatcctg
      541 acgcctctgg ccatcgaccc ggcgcacccc ttcccgttca tcccgaacct gggcttctcg
      601 ctggcgctga agctgacccg tcagtcggat ggacgcacgc tctacgcgct cgtgccggtg
      661 ccgacccagg tgcagcgctt ctggcccctg cctttcgaag agggcgagcg cagccgccgg
      721 tacatcgcgc ttgagagcct gctggcgctt ttcactgacc acctcttccc cgggatgcag
      781 gtcgaggcgc gcggcgcctt ccgcatcatc cgcgacagcg acgtcgagat cgaggaggag
      841 gccgaggacc tcgtccgcga gttcgaggcg ctgctgaagc agaggcgtct cggctcggtg
      901 gtgcgcatca agatcgaggc ggccatgccc gccgatctgc gcaccttcat catcgccaac
      961 ctgcgcgccg agccccagga catcagcctg gtcgaaggca tgctggggct ggcgcagctc
     1021 tctcagctga tcccgcccga ccgccaggac ctcaagttcc caccctacga gccccggttt
     1081 ccagagcggg tccgggacca cggcggcgtc tgcttcgcgg ccattcgcga gaaggacatc
     1141 ctggtccacc acccgttcga gtcgttcgac gtggtggttc agttcctgcg ccaggcggcg
     1201 accgatcccg acgtgctggc gatcaagcag acgctgtacc ggaccagcaa ggacagcccg
     1261 atcgtggccg ccctgatcga ggccgcggag aacgggaaga acgtcaccgc gctcatcgag
     1321 atcaaggcgc gttttgacga agaggcgaac ctgaagtggg cccgggatct ggagcgcgcc
     1381 ggcgtccacg tcgtcttcgg cttcatcgag tacaagacgc acgccaagct gtcgctcgtc
     1441 gttcggcgag agggcgagtc gctcaccacc tactgccact tcggaacggg caactaccac
     1501 ccgcagacgg cgcgcgttta caccgacctg tcgctcttca cgacgcacgc gccgctcggc
     1561 cgcgacagcg cacgcctgtt caacttcatc accggttacg ccttgccgga agcgctggaa
     1621 ggtctggcgt tctcgccggc gaccttgaag tcgacgctgc tgacgcgcat cgaccgagag
     1681 gtcgagaacg cgggcaaggg caaaccggcg gcgatctggg ccaagctgaa ttcgctggtc
     1741 gatcccgtga tcatcgacgc cctctaccgt gcgagccagg cgggcgtccg catcgagctg
     1801 gtgatccgcg gcatctgctg cctgcggccg caagtgccgg gcctgtcgga gaacatccgc
     1861 gtcaagagca tcgtcggccg cttcctggag cacagccgca tcgtcgcctt cgccgatggc
     1921 cgccgcatgc cgagccaccg cgcgcgcctg tacatctcct cggccgactg gatgccgcgg
     1981 aacctggacc ggcgcgtcga gtcgctggtt ccggtgatca atccgaccgt tcatcgccag
     2041 atcctggatc agatcatggt cgccaacctg aacgat
//
LOCUS       DASVTM010000464         5113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_999921,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000464 DASVTM010000000
VERSION     DASVTM010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999921"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF633_12810"
     CDS             <1..267
                     /locus_tag="VF633_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8471986.1"
                     /translation="LSDYNTGSDLHPSKAQDSYTLVNARIGIGAESGRWALEAWAQNL
                     TDEDYLQVGFNGPFQVDENNDAISVYNAFLGAPRTYGLTLRLAY"
     gene            complement(368..1513)
                     /locus_tag="VF633_12815"
     CDS             complement(368..1513)
                     /locus_tag="VF633_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8471987.1"
                     /translation="MNFDLSEEQSLLNDSVSGFLKARYDFEARKKIVASEAGWSPAIW
                     QALAEELGVVGVMAPEAAGGLDGGPVEAMVLMEAMGRALVVEPFLETALTSVSLLKRA
                     GGARADALLEQIVAGAAIVAFAHQEAKGRHQLLSVATTATPDGDGWKLTGSKAVVSAA
                     PWATHLIVSARTSGTDPRDPAGLSLFLIEVATSSAGLSVRGYPTIDERHAAEIDLEGL
                     TLPADALLGEVNQAHDALIAPVMDEAVAALCSEAVGVLRELHAQTLDYAKQRKQFGRP
                     IGEFQVLQHRLVDMFIAVEQAVSLTLMATLKLDAPAAERARAVSAAKVGVSKALKFVS
                     QNAVQTHGGIGMTDELALSHYFRRATAIELQLGSPQAHLKRFISLAA"
     gene            complement(1515..2696)
                     /locus_tag="VF633_12820"
     CDS             complement(1515..2696)
                     /locus_tag="VF633_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8471988.1"
                     /translation="MNLDYSPEELAFRDEVRAFIAAEYPPELRAKQDAGEELDDQDFL
                     SWHRILAKRGWSAPAWPVEYGGPGLTQTQRFLMSEEMAAADTAPILPFGVHMVGPVIY
                     TFGTPEQKARWLPPTLAGDIWWCQGYSEPGAGSDLASLKTRAVRSTDAEGEHYIVNGQ
                     KAWTTLAQFADWGFFLVRTDPDAKPQQGISFLLIDMKSPGITVRPIITLDGSHEVNEV
                     WLEDVRVPVENRIFKENEGWTCAKFLLAHERTGIAGVARAKRGLEKLRKVAAAEGVID
                     DPVFAAELADLEIDLLALEYSELRTLARESSGKGPGPESSLLKIKGTEIQQRLTEMTV
                     EALGSYALPDARGLGNVGPVGPDYAGRIAPTYFNMRKVSIFGGSNEIQRNIIAKMVLG
                     L"
     gene            complement(2693..4447)
                     /locus_tag="VF633_12825"
     CDS             complement(2693..4447)
                     /locus_tag="VF633_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8471989.1"
                     /translation="MTAAVAETPAVWPAMTLAQAHAMLTQPGSPFEIETIEINGRPTK
                     SWKNAPPTLMHSFMAGRMHGDRTFIVHEDERITFEAFARATLALAADLQARGVQKGDR
                     VAIAMRNLPEWPVAFFAASLIGAIVVPLNAWWSGSELAFGLRDSGSKLAIFDIERLAR
                     IADHRDALPDLQHMLVSRGYPMTTDARVTRLEAVIGAPNDWAALPVGTMPAVALGPDD
                     LSAIFYTSGTTGKPKGALATHRNAGTGVMAGLFSYSRAFLRRGDTPPQPDPSAPQKSG
                     LVSIPFFHTTGCNALLLPALINGQKIVCQRRFDAAEALMLIEREKINSIGGVPAVAIQ
                     LLQHPDRDKYDLSSLELVTYGGAAAPTALVGEIATQTTGSPGSGWGMTETSATHTHHL
                     GEDYLNRPDSCGPALPVGDLRVVDPDGNDLPVGEVGELLAYGPNVVKGYWNRPDDTAE
                     TFVDGWVHTGDLARLDEEGFCFIVDRKKDIIIRGGENIYCQEVEDVLFEHPAVAEAAL
                     IARLHPVLGEEPVAVVSLAPEMSVEPEELRALAASRLAAFKVPVTVFILPHPLPRNAA
                     GKVVKADVKALVLTEPAA"
     gene            4604..>5113
                     /locus_tag="VF633_12830"
     CDS             4604..>5113
                     /locus_tag="VF633_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8471990.1"
                     /translation="MTVTAAAPRQTQRYEAKRDAILDAAARLFNRKGLKGATLGDVAE
                     AVGLITTSVTYYYRRKEDLAAACLLRTIDILQEVVATASIAPTPEARIAAFLDAYFEV
                     LADIAAGRRPEPINFYDIRALTGPQVGVVKDAFNDLFRAFRTLLRPEVGPLFDRLEEN
                     GRTHLFFSQI"
BASE COUNT          854 a         1683 c         1709 g          867 t
ORIGIN      
        1 ctgagcgact acaacacggg atcggacctt catccgtcca aggcgcagga ctcttatacc
       61 ctcgtcaacg cccggatcgg catcggcgcg gaaagcggcc gctgggcgct cgaagcctgg
      121 gcgcagaacc tgaccgatga agactatctt caggtcggct tcaacggccc gttccaggtc
      181 gacgagaaca acgatgcgat cagtgtctac aacgccttcc tcggcgcgcc ccggacatat
      241 ggtctgacgc tacggctcgc ctactgattc ccgagcccac taagccgccc cggtcgaaag
      301 gccggggcgg cattcttttg gcgctaccgc cccttgggct acttgagcgc gaagagctgc
      361 caactgccta ggccgccagc gagatgaacc gcttcagatg cgcctgcggg ctgccgagct
      421 ggagctcgat ggcggtggcg cggcggaagt agtggctcag cgccagttcg tcggtcatgc
      481 cgatgccgcc atgggtctga accgcattct gcgacacgaa cttcagcgct ttcgagaccc
      541 cgaccttggc ggcggagacg gcgcgggcgc gctcagccgc aggggcgtcc agcttcagcg
      601 tcgccatcag ggtcagggaa accgcctgtt cgacagcgat gaacatgtcg accagccggt
      661 gctgcagcac ctgaaactcg ccgatcggac ggccgaactg cttgcgctgt ttggcgtagt
      721 cgagcgtctg ggcatgaagc tcgcgcagta caccgacggc ttccgaacac agcgccgcaa
      781 cggcttcgtc cataaccggt gcgatcaagg cgtcatgcgc ttgattgacc tcgccaagca
      841 gggcgtcggc gggcagggtc aggccttcca gatcgatctc ggcagcatgg cgctcgtcga
      901 tagtcggata gccgcgcacc gacagacccg cgctcgaagt cgcgacctcg atcaggaaca
      961 ggcttagacc cgccggatcg cgcggatcag tcccgctggt ccgagcgctg acgatcaggt
     1021 gtgtagccca gggcgcggcg ctgaccacgg cctttgaacc ggtcagcttc cagccgtcgc
     1081 cgtctggcgt ggcggtggtc gcgaccgaca gcagctggtg gcgacccttg gcctcctgat
     1141 gcgcgaaggc gacgatggct gcgccggcaa cgatctgctc aagcaaggcg tcggcgcgcg
     1201 cacctcccgc ccgcttcagc agcgacaccg aggtcagcgc cgtctcgagg aacggttcga
     1261 ccaccagcgc gcggcccatg gcctccatca gcaccatggc ctcgaccggg ccgccgtcca
     1321 gaccgcctgc ggcctcgggc gccatgacgc cgacaacgcc gagttcctcg gccagcgcct
     1381 gccagatcgc ggggctccag ccggcctcgg aggcgacgat cttcttgcgc gcctcgaagt
     1441 cgtagcgggc cttgaggaag ccggagacgg aatcgttgag cagcgactgc tcttcgctca
     1501 ggtcgaaatt catctcagag ccccagaacc atcttggcga tgatattgcg ctggatctcg
     1561 ttcgaaccgc cgaagataga gaccttgcgc atgttgaaat aggtcggcgc gatgcgaccg
     1621 gcgtagtcgg gaccgaccgg ccccacgttg ccgaggcccc gcgcgtcggg cagggcgtag
     1681 gaccccagcg cctcgaccgt catttcggtc agccgctgct ggatctcggt gcccttgatc
     1741 ttgagcagcg acgattccgg ccccggtccc ttgcccgaac tctcgcgggc gagggtgcgc
     1801 agttcgctgt attccagcgc cagcaggtcg atctccagat cggccagttc tgcggcgaag
     1861 accggatcgt cgatgacgcc ttccgccgcc gcgaccttac gcagcttctc cagaccgcgc
     1921 ttggcccggg caacgcccgc gatgccggtg cgctcatgcg caagcaggaa tttggcgcag
     1981 gtccagccct cgttctcctt gaatatccgg ttctcgaccg ggacccgcac gtcctccagc
     2041 cagacctcgt tgacctcatg ggaaccgtcg agcgtgatga tcgggcgcac ggtaatgccg
     2101 gggctcttca tgtcgatcag caggaaggag atgccctgct gcggcttggc gtccgggtcg
     2161 gtgcggacga ggaagaagcc ccagtcggcg aactgcgcca gcgtcgtcca ggccttctgg
     2221 ccgttgacga tatagtgctc tccctcggcg tccgtgctcc gaaccgcacg ggtcttgagg
     2281 ctggcgaggt cggaaccggc gcccggctcg gaatagcctt ggcaccacca gatatcgcca
     2341 gccagcgtcg gcggcagcca gcgcgccttc tgctccggcg tgccgaatgt atagatgacc
     2401 ggcccaacca tgtggacgcc gaacggcagg atcggcgccg tgtccgccgc cgccatctct
     2461 tccgacatca ggaagcgctg ggtctgggtc aggcccgggc cgccgtattc gaccggccag
     2521 gccggagcgc tccagccgcg tttggcgagg atgcggtgcc aagacaggaa gtcctgatcg
     2581 tcgagctcct cgccggcgtc ctgcttggcg cgcagctcag gcggatattc tgcagcgatg
     2641 aaggcgcgga cctcgtcgcg gaaggcgagt tcttcggggg agtagtcgag gttcacgcgg
     2701 cgggctccgt caggacgagg gctttgacgt ccgccttcac caccttgccg gcggcgttgc
     2761 ggggcagggg atgcggcagg atgaagacgg tcacggggac cttgaaggcg gcgaggcgcg
     2821 aggcggcaag cgcccgtagt tcctccggct cgaccgacat ttccggggcc agagacacca
     2881 cggcgaccgg ctcctcgccg agcacgggat ggaggcgagc gatcagggcg gcctcggcga
     2941 cggcggggtg ttcgaacagg acgtcctcca cctcctgaca gtagatgttc tcgccgccgc
     3001 ggatgatgat gtccttcttg cgatcgacga tgaagcagaa gccttcctcg tccagacgcg
     3061 ccagatcgcc ggtatggacc cagccgtcga cgaaggtctc cgcggtgtca tccggccggt
     3121 tccagtagcc cttcacgacg ttggggccat aggcgagcag ttcgccgacc tcgccgacgg
     3181 gcaggtcgtt tccatcagga tcgacgacgc gcaggtcacc aacgggcagg gccggaccgc
     3241 agctgtcggg gcggttcaga tagtcctcgc ccagatggtg ggtgtgggtg gccgaggtct
     3301 cggtcatgcc ccagcccgag ccgggcgagc cggttgtctg ggtagcaatc tcgccgacca
     3361 gcgcggtggg cgccgcagcc ccgccgtagg tgaccagttc cagcgaggaa aggtcgtact
     3421 tgtcacggtc cggatgctgc agcagttgga tggccacagc cggcacgcct ccgatggagt
     3481 tgatcttctc gcgctcaatc agcatcaggg cctcggccgc gtcaaagcgg cgctggcaga
     3541 cgatcttctg gccgttgatc agggcgggca gcagcagggc gttgcagccg gtagtgtgga
     3601 agaacgggat agagaccagc cccgacttct gcggcgcgct cgggtcgggc tgtggcggcg
     3661 tgtcaccccg gcgcaggaag gcgcggctgt aggagaacag acccgccatt acgccggtgc
     3721 cggcgttgcg gtgggtcgcc agcgcaccct tgggcttacc ggtcgtgccc gaggtgtaga
     3781 agatcgccga caggtcgtcg gggcccagag cgacggcggg catggttccg acgggcaggg
     3841 cggcccagtc gttcggggcg ccgatgacgg cttcgaggcg ggtgacccgc gcatccgtcg
     3901 tcatcgggta cccgcgactg accagcatat gctggagatc gggcagggcg tcgcgatggt
     3961 ctgcgatccg ggccagacgt tcgatgtcga agatggccag cttgctgccg gaatcccgca
     4021 gcccgaacgc tagctccgac ccgctccacc aggcgttcaa cggcactacg atcgctccga
     4081 tcagcgaggc ggcgaagaag gcgaccggcc attccggcag gttgcgcatg gcgatcgcca
     4141 ccctgtcacc cttctggaca ccgcgcgcct gtagatcggc ggcgagggcc agggtggcgc
     4201 gggcgaaggc ctcgaaggtg atccgctcgt cctcatgcac gatgaaggtg cggtcgccat
     4261 gcatccgtcc cgccatgaag gaatgcatca gggtcggcgg ggcgttcttc cagctcttcg
     4321 tcgggcgtcc gttgatctcg atcgtctcga tctcgaacgg cgagcccggt tgcgtcagca
     4381 tggcgtgcgc ctgcgccagc gtcattgccg gccacacggc gggggtttca gcgaccgcag
     4441 cagtcatcaa gcgttcctct ttgcggccgc ttgtgcggct ctggcttcgc gcggtgggca
     4501 attatgtttc cgtaacggaa atcccaagcc aagcgcgcaa tctcggtata ggctgcaact
     4561 tgctccatac tcaatcttcg aagcaaggcc tttgacgacc cgaatgaccg ttaccgccgc
     4621 cgctccccgc cagacccaac gctatgaagc caagcgggat gcgatcctcg acgccgccgc
     4681 gcggctgttc aaccgcaagg gtctcaaagg cgcgacgctc ggcgacgtgg ccgaggcggt
     4741 cggcctgatc accaccagcg tgacctacta ttaccggcgc aaggaagacc ttgcggcggc
     4801 ctgtctgctg cgcaccatcg acatcctgca ggaggtggtc gccaccgcca gcatcgcgcc
     4861 cacgccggag gcgcggatcg ccgccttcct cgacgcctat ttcgaggtgc tggccgatat
     4921 cgctgcggga cgccgtccgg agccgatcaa tttctacgac atccgtgctc tgaccgggcc
     4981 gcaggtgggg gtggtcaagg atgcgttcaa cgacctgttc cgcgccttcc gcaccctgct
     5041 gaggcccgag gtgggaccct tgttcgacag gctggaggag aacgggcgga cgcatttgtt
     5101 cttctcccag att
//
LOCUS       DASVTM010000465         7124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_149015,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000465 DASVTM010000000
VERSION     DASVTM010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149015"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /gene="ccmC"
                     /locus_tag="VF633_12835"
     CDS             <1..643
                     /gene="ccmC"
                     /locus_tag="VF633_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX8471991.1"
                     /translation="VLLAIGTWLSFAAPGDYQQSDTVRIMFVHVPAASLGTMVYAALG
                     VSSFFALVFRHPLADAAARAAALPGAAFTALALITGSLWGQPMWGTWWVWDARLTSVL
                     VLFLFYLGYMALRSSIDDEAKAARAAAVLGLVGLINLPIVKFSVDWWNTLHQPASLLR
                     EGGSSLAPVFLWPLLTMMAAYAALFLAVWLTSIRTEIVRRRVITLRARAALEA"
     gene            648..800
                     /gene="ccmD"
                     /locus_tag="VF633_12840"
     CDS             648..800
                     /gene="ccmD"
                     /locus_tag="VF633_12840"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX8471992.1"
                     /translation="MLDLDMTPYAAFVWPAWGISAVVLAALVARAVIASRRWKRELKT
                     LEDDRP"
     gene            797..1327
                     /locus_tag="VF633_12845"
     CDS             797..1327
                     /locus_tag="VF633_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX8471993.1"
                     /translation="MMRWLAIVPLIVIAALAVLFIGWSLRHDPTYKPDAAVGQMAPET
                     VLPLLNGSVAGPSNVDLRTVAMGRPVIVNMFASWCAPCRVEHPRLMALKAKGVRIIGI
                     AYKDEPEATAKMLEDLGNPFDLILVDHEGRAGLDLGISGVPESFAIDRTGKIVAKSSG
                     PIVTDADVARLVAAIQ"
     gene            complement(1653..2258)
                     /locus_tag="VF633_12850"
     CDS             complement(1653..2258)
                     /locus_tag="VF633_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8471994.1"
                     /translation="MVKSGKAKRLGTLADSPSHLMHRVLQLALDIYSDEAGPDGPTQR
                     QFAVMEAVAAKSGLTQTDLVRATGIDRSTLADLVSRMTTKGLLERARSVVDARAKAVT
                     LSDTGKALLESARPKVEAADKRIMALLPKGKREGFIELLVDLANAADAAPDLARAEAK
                     ATKKAAKEVKRAEKLARKSVEGAVKKAPKAKKSKPVLEPAS"
     gene            complement(2422..3027)
                     /locus_tag="VF633_12855"
     CDS             complement(2422..3027)
                     /locus_tag="VF633_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HEX8471995.1"
                     /translation="MTDQTAPETKRPQWIRQAVDFGALLCFAIAYFVTKDMIKATWVL
                     VAASAVALIVGYLVERRFAPLPLITGAFALVFGGLTIFFHDESIIKIKLTVQNAMLAI
                     VLLGSLPLNKSPFKYLLGDAIKVTDQAWRGLTLRYGLYFGAIAIANEIVWRTQPDGTW
                     VQFRVGLWILACVFGLIQIPYIMKHMIKDEEPGPTSPDTGL"
     gene            complement(3030..3983)
                     /gene="ftsY"
                     /locus_tag="VF633_12860"
     CDS             complement(3030..3983)
                     /gene="ftsY"
                     /locus_tag="VF633_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX8471996.1"
                     /translation="MNDTPKKGWFQRLSDGLARSSKQITETVVGSLVKEPLDQAALDR
                     LEEHLLESDLGPAATDRIVARFRDLRFGKVSDEREVKEALAEAVAAELLPRQASFDPL
                     GPDGVAGGIKPFVVLFVGVNGSGKTTTLGKIAADLTGKGAKVMIVAGDTFRAAAREQL
                     KVWAERSGAEFESRRDGADPAGLAFDAYTKARAESYDVVLIDTAGRLQNKSALMDELL
                     KIVRVLKKIDPDAPHETLLVLDATVGRNALAQEQIFGRAAYVSGIVMTKLDGTARGGV
                     LVPVAQASDAPIKLIGVGEGIEDLQPFDARAFSRSLVGLED"
     gene            4092..6134
                     /locus_tag="VF633_12865"
     CDS             4092..6134
                     /locus_tag="VF633_12865"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX8471997.1"
                     /translation="MAEPHPFYSTRTHGFVRVAAATPVVHIADPKANAEEHEALIRQA
                     GEQGVDLMVFPELSLSAYAIDDLLMQAALLEEVERRIHDLARVTAETGVIAVIGAPIR
                     TGDRLYNCAVVLGEGRVLGVVPKTYLPNYREYYEKRWFSPADDHLPDLGEEWGAFGTR
                     LIFAAENRPGFVFGVEVCEDYWAPLPPSTRQAMAGARILLNLSASNIVIGKSDDRALL
                     CASQSARAMAAYVFAASGWGESTTDLAWDGQATIHELGARIADGERFSLESHLTVADI
                     DVDRIGLERLRTGTFADCARRELGEDQTFDTVLFEAREDAGDTPLIRPLDRFPFVPDD
                     PARLDQDCYEAFNIQVQGLMRRLKSTGSKKIIIGVSGGLDSTQALLVACRAFDRLGKP
                     RTDILGFTMPGFATSEGTKSNAWALMNALGITGAEIDIRPAAQAMLEGIGHAFGRGEA
                     VHDITFENVQAGLRTDYLFRLANQNSGFVLGTGDLSELALGWATYGVGDHMSHYNVNG
                     GVAKTLIRHLIRWVAGLNEMAGAADVLNSILGTEISPELVPAGADGKIQSTEGTVGPY
                     ALNDFFLFYVTRFGLKPSKVAYLCHQAWGDASRGEWPPHHPEADHLAYDLATIKFWLR
                     KFLIRFFQTSQFKRSALPNGPKVVTGGSLSPRGDWRAPSDGNARVWLDELDANVPD"
     gene            6344..6568
                     /locus_tag="VF633_12870"
     CDS             6344..6568
                     /locus_tag="VF633_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEX8471998.1"
                     /translation="MTDTPTKDSNGNVLADGDSVTLIKDLKVKGSGGVTLKRGTVVKN
                     IRLTGDEAEIEANVDKVRGLVLRTEFVRKA"
     gene            complement(6573..>7124)
                     /locus_tag="VF633_12875"
     CDS             complement(6573..>7124)
                     /locus_tag="VF633_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8471999.1"
                     /translation="KMSMPSDPRALARMVAQLSRELGAAVKAYGGAGGSMTDLASNAS
                     TAAPAAEGQTASAVGEPAQAAPSADVVTAEEAGEVEGADNEEKTTPTDPYRQAFEKQQ
                     AQSADQARKAEGAKADSEFVTTVKGLVADLKAMAKKAEADAKRSGENGSDADAAKAAL
                     ETVDTQVDAVAKALTGGMVSLTI"
BASE COUNT         1181 a         2327 c         2281 g         1335 t
ORIGIN      
        1 cgtactgctg gcgatcggaa cctggctgtc gttcgcggcg cccggcgact accagcagag
       61 cgacaccgtc cgcatcatgt tcgtccacgt gcccgccgcc tcgttgggca cgatggtcta
      121 tgcggcgctg ggtgtttcca gctttttcgc cctcgtgttc cgccatccgc tggccgacgc
      181 cgccgcccgg gctgccgcct tgccgggcgc agccttcacg gctctggctc tcatcaccgg
      241 ctcgctttgg ggccagccga tgtgggggac atggtgggtc tgggatgcgc gcctgacctc
      301 ggtgctggtg ctgtttctgt tttatctcgg ctacatggcc ctgcggtcgt cgatcgacga
      361 tgaagccaag gccgcgcgcg ccgccgccgt tctgggtctg gtcgggctga tcaacctgcc
      421 gatcgtgaag ttttccgtgg actggtggaa cacgctgcat cagccggcgt cgctgctgcg
      481 cgagggcggc tccagtctgg cgccggtgtt cctgtggccg ctcctgacga tgatggcggc
      541 ctatgcggcg ttgtttctgg cggtgtggct gacatcgatc cgcacggaga tcgtccgtcg
      601 ccgggtcata acccttcgcg cccgcgctgc gctggaagcc tgaagccatg ctcgatctcg
      661 acatgacgcc ttacgccgcc ttcgtctggc cggcctgggg catcagcgcc gtcgttcttg
      721 ccgcgctcgt ggcccgggcg gtgatcgcct ctcgccgctg gaagcgtgag ctgaagacac
      781 tcgaggacga ccgcccatga tgcggtggct ggcgatcgtg cccctcatcg tgatcgcagc
      841 tctcgcagtg ctgttcatcg gctggtcgtt gaggcacgac cctacctaca agcccgacgc
      901 cgccgtcggt caaatggcgc ccgagacggt gttgccgctc ctgaacggct cggtcgctgg
      961 accgtcgaac gtcgatttga ggactgtcgc catgggccgt ccggtgatcg tcaacatgtt
     1021 cgccagctgg tgcgcgccgt gccgggtcga gcatccccgc ctgatggccc tgaaggccaa
     1081 gggcgtccgg atcatcggca tagcatacaa ggacgaaccc gaggccacgg cgaaaatgct
     1141 cgaagacctc ggcaacccct tcgacctgat cctcgtcgac cacgaaggac gcgccgggct
     1201 ggatctgggt atctccggcg tgccggaaag cttcgcaatc gatcgcaccg gcaagatcgt
     1261 cgccaagtcg tccggaccga ttgttactga cgccgacgtt gcccggctgg tggccgcgat
     1321 ccagtagtca acgctttcgg ttacgacgcg ctcatgcggc aagccgccga gtgggaactt
     1381 tagcgcctca agaaggccgt taaccttttt tcgagagatt tcgttaacct tgtcggcatg
     1441 agcacgattc gccccaacct gcccgcagtg cagccgcaac cctctgcccc agcgcggtca
     1501 gcccaagctg catttttccg cgcggcgctg gatcaggtca atgccgttca ggttcagacg
     1561 acgccggttc aaacgacatc tggcgcccag acatccgcac cctccgatac cggacgatcc
     1621 atgcgccccg gcagcctgct cgatataaga atctaggagg ctggctcgag gaccggcttc
     1681 gactttttgg cttttggcgc cttcttgacg gcgccctcta ccgacttgcg ggcaagtttt
     1741 tcggcacgct tcacttcctt ggccgccttc ttcgtcgcct tggcttcggc tctggcgaga
     1801 tcaggcgcgg cgtctgcggc attggccaga tcgaccagaa gctcgataaa gccctcgcgc
     1861 ttgcccttgg gcaacagggc catgatgcgt ttatcggcgg cctccacctt gggtcgggcg
     1921 ctttcgagca gggccttgcc ggtgtccgac agcgtcaccg ccttggcgcg cgcgtccacg
     1981 acggagcgcg cccgctccag cagtcccttt gtggtcatgc gcgagaccag atccgccagt
     2041 gtcgaacggt cgatgccggt ggcccggacg agatccgtct gtgtcaggcc agatttcgcc
     2101 gccacggcct ccatcacggc gaactgacgc tgcgtcggtc cgtccgggcc cgcctcgtcg
     2161 gaatagatgt cgagcgccag ctgcagcacg cggtgcatca ggtgactggg cgaatccgcc
     2221 agcgttccca gacgtttggc cttgccggac ttcaccatgg tcaacatccc tttgaggcgt
     2281 cttgccgcct ctaccgtgaa cggagggtta tcagcgtggc tttacggttt gacgacactg
     2341 ttgcctgagg cgcttcgcgc cgcgctatgc tcggcccgcg gggcctcgcg aattgagcgc
     2401 aaagatggtc tttgacggct attatagtcc ggtgtcgggg gaggttggac cgggctcttc
     2461 gtccttgatc atatgcttca tgatgtaggg gatctggatc aggccaaaga cgcaggcgag
     2521 gatccacagg ccgacgcgaa actgcaccca ggtgccgtcc ggctgggtcc gccagacgat
     2581 ctcgttggcg atagcgatgg ctccaaaata caggccgtac cgcagggtca gccccctcca
     2641 ggcctgatct gtgaccttga tcgcgtcgcc cagtagatat ttgaacggcg acttgttcag
     2701 cggcagggag cccagcagca cgatggccag catcgcgttc tgcaccgtca gcttgatctt
     2761 gatgatgctc tcgtcgtgga aaaagatcgt cagcccaccg aacacgagcg cgaaggcgcc
     2821 ggtgatcagt ggcaggggcg cgaaccgccg ctcgaccaga taacctacga tcaaagccac
     2881 ggccgaggct gcaaccagca cccaggtggc cttgatcatg tccttggtga cgaaataggc
     2941 gatggcgaaa caaagaagcg cgccgaaatc gacggcttgc cgaatccact gcggacgttt
     3001 ggtttcaggc gcggtctggt cggtcatgtt cagtcctcga gcccgaccag cgagcgggaa
     3061 aaggcgcggg cgtcgaaggg ctgcagatcc tcgatcccct cacccacgcc gatcaacttg
     3121 atcggggcgt cggacgcctg ggcgaccgga accagaacgc cgccgcgcgc cgtgccgtcc
     3181 agcttggtca tcacgatgcc cgagacataa gccgcccggc cgaagatctg ttcctgagcc
     3241 aaggcgttgc ggccaacggt cgcgtccagc accagcagcg tctcatgcgg ggcgtccgga
     3301 tcgatcttct tcagcacgcg aacgatcttg agcagttcgt ccatcagggc ggacttgttc
     3361 tgcagccggc cggcggtgtc gatcagcacg acgtcatagc tctcggcccg cgccttggta
     3421 taggcgtcga aggccagccc ggccggatcc gcgccgtcgc gacggctctc gaactcggcg
     3481 cccgagcgtt cagcccagac cttgagctgt tcgcgggcgg cggcgcggaa ggtgtccccg
     3541 gccacgatca tgaccttggc gcccttgccg gtcaggtcgg cggcgatctt gcccagcgtc
     3601 gtggtcttgc ccgaaccgtt gacgccgacg aacaacacga cgaacggctt gatcccgcca
     3661 gccacgccat ccgggcccag aggatcgaaa ctggcctgac gcggcagaag ctcggcggcg
     3721 acggcttcgg ccagggcttc cttgacctcg cgctcatccg acaccttgcc gaagcgcagg
     3781 tcgcggaagc gcgcgacgat ccgatcggtc gctgcggggc cgaggtcgct ctccagcaga
     3841 tgctcttcca gccggtccag cgccgcctga tccagcggtt ccttgaccag actgccgacg
     3901 acggtttcgg tgatctgttt ggaggagcgg gccagaccgt cgctgagacg ctggaaccag
     3961 ccctttttcg gcgtatcgtt catgcgacgg ctttagcggt ccggcgttca caggtcacgg
     4021 actaacatag cctgtatagt ggagccgcga ctttggctct atatgagcat aagccctcga
     4081 aggaccgcgc cttggccgaa ccccatccgt tctattcgac ccgcacccac ggtttcgtcc
     4141 gtgtggccgc tgcgaccccg gtcgtccaca tcgccgatcc caaggccaac gccgaggaac
     4201 atgaggccct gatccgccag gcgggcgagc aaggtgtgga cctgatggtc tttcccgaac
     4261 tgtcgctgag cgcctatgcg atcgacgacc tgttgatgca ggcggctctg ctggaggagg
     4321 tcgagcggcg gatccatgat ctggcgcgcg tgactgcaga aaccggggtc atcgccgtca
     4381 taggcgcgcc gatccgcacg ggcgaccgcc tttacaactg cgccgtcgtg cttggagaag
     4441 gacgtgtcct tggggtcgtg cccaagacct atctgccgaa ttatcgggaa tactacgaga
     4501 agcgctggtt cagcccggcc gacgaccatc ttcccgacct cggcgaagaa tggggggcgt
     4561 tcgggacgcg gctgatcttt gcggcggaga accggccggg gttcgttttc ggcgtagagg
     4621 tttgcgagga ctattgggcg cccctgccgc cgtccacgcg tcaggccatg gccggcgcga
     4681 gaattctgct gaacctgtcg gcatcgaaca tcgtcatcgg caagtccgac gatcgcgcct
     4741 tgttgtgcgc cagtcagtcg gcgcgcgcga tggcagccta tgtctttgca gcttccggct
     4801 ggggtgaaag caccacggac ctggcctggg acgggcaggc gacgatccac gaactcggcg
     4861 cgcgcatcgc cgacggcgaa aggttttcgc tggaatccca cctgacggtg gccgatatcg
     4921 acgtcgaccg gatcgggctc gagcgcctgc gtaccgggac gttcgccgac tgcgcccggc
     4981 gcgagctcgg cgaggatcag acgttcgaca cagtcctgtt cgaggcccgc gaagacgccg
     5041 gcgacacccc tctcatccgc ccgctcgacc gctttccgtt cgtcccggac gatcccgcgc
     5101 gtctggatca ggactgctac gaggcgttca acatccaggt tcaggggctg atgcggcgcc
     5161 tgaaatcgac cggatcgaag aagatcatca tcggcgtgtc gggcgggctg gattcgacgc
     5221 aggccctgct ggtcgcctgt cgggcgttcg atcggctggg caagccgaga accgatattc
     5281 tcggcttcac catgcccggg ttcgcgacgt cggagggcac gaaatccaac gcctgggcgc
     5341 tgatgaacgc tctggggatc accggtgctg agatcgacat ccggcccgcc gcgcaggcca
     5401 tgctggaggg catcggtcac gctttcgggc ggggcgaggc ggtccacgac atcaccttcg
     5461 agaacgtgca ggccgggctg cggaccgact atctgttccg gctggccaac cagaacagcg
     5521 gcttcgtgct tggaaccggc gacctgtcgg aactggcgct cggctgggcg acctatggcg
     5581 tcggcgatca tatgagccat tacaacgtca acggcggggt ggcgaaaacc ctgatccggc
     5641 atctgatccg ctgggtcgcg gggctgaacg agatggctgg cgcagccgat gtgctgaaca
     5701 gcattcttgg cacagaaatt tcgcccgagc tcgtccccgc cggcgccgac ggaaagatcc
     5761 agtcgacgga gggaaccgtc ggcccctatg ccctgaacga cttcttcctg ttctacgtca
     5821 cccgtttcgg cctcaagccg tccaaggtcg cctacctctg tcatcaggcg tggggtgacg
     5881 cgtcgcgggg agaatggccg ccccatcatc cggaggcgga tcacctcgcc tatgacctgg
     5941 cgacgatcaa attctggctc cggaaattcc tgatccgctt cttccagacc agccagttca
     6001 agcgctcggc cctgccaaac ggacccaagg tggtgactgg cggatcgctg tctccacgcg
     6061 gagactggcg cgccccgtcc gacggcaatg cgcgcgtctg gctggatgaa ttggacgcca
     6121 acgtaccgga ttgacgtttc acctctccat tcgcgaaggc gaatggagag gacaggttgg
     6181 cgcgtagcga cgaccaggag agggcgacgc cggcggatgg gactagcgac tctgcggata
     6241 gacctagtcg ccctcacctg atcgcttacg cgatctgtcc tctccatcgc cctccggcga
     6301 tggagaggtg acctcagccg gtccgcccgc gagaagcttc gccatgaccg acaccccgac
     6361 caaagactcc aacggcaacg tgctcgctga cggcgacagc gtgaccctga tcaaggacct
     6421 caaggtcaag gggtcgggcg gagtgacgct gaagcgcggg accgtcgtca agaatatccg
     6481 gctgaccggc gacgaggccg agatcgaggc caatgtcgac aaggtgcggg gtctggtgct
     6541 gcggaccgag ttcgtccgca aggcctgagg ccctagatcg tcagggagac cataccgccg
     6601 gtcagcgcct tggcgacggc atcgacctga gtgtccactg tctcaagcgc cgccttcgct
     6661 gcgtcggcgt ccgaaccgtt ctcacctgat cgcttggcgt cggcctcggc cttcttcgcc
     6721 atggctttca gatcggcgac gaggcctttc acggtcgtga cgaactccga gtccgccttg
     6781 gctccctccg ccttgcgggc ctgatccgcc gactgggcct gctgcttctc gaacgcctgg
     6841 cgatagggat cggtcggggt ggttttctcc tcgttgtcag caccttcgac ttcgccggcc
     6901 tcctcagcgg tgaccacatc agccgaaggc gcggcctgcg cgggctcccc gacggccgag
     6961 gcagtctgtc cctcggcagc cggcgccgca gtagaggcat ttgaggccag atcggtcatc
     7021 gacccgccgg cgccgccgta ggctttgacc gctgcaccca gctcgcggga cagttgggcg
     7081 accatccggg cgagcgcgcg gggatcggac ggcatcgaca tctt
//
LOCUS       DASVTM010000466         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_413563,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000466 DASVTM010000000
VERSION     DASVTM010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413563"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..628)
                     /gene="ruvA"
                     /locus_tag="VF633_12880"
     CDS             complement(11..628)
                     /gene="ruvA"
                     /locus_tag="VF633_12880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX8472000.1"
                     /translation="MIGRLRGVLAEIEEGHCLIDCAGVGYVVACGARTLGRLPAPGDE
                     ATLHVHSQWSEDGGPRLYGFLTRDERRAFTTLLAIQGVGPKAALSVLDVLPPGELAGA
                     VARDDKAAVGRANGVGPKLALRIVTELKGKSLGDISFTPNAPGVHTEIAAPVPSVSGE
                     AVSALLGLGIAEINARRAVDQALIRLGEDAELPAVIRAALQELGR"
     gene            complement(625..1182)
                     /gene="ruvC"
                     /locus_tag="VF633_12885"
     CDS             complement(625..1182)
                     /gene="ruvC"
                     /locus_tag="VF633_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX8472001.1"
                     /translation="MPNMMIQNGPIGGPVRILGLDPGLRRTGWGVIVSEGSRLRWIAH
                     GVVTPDERAPFSERLLHLLEAVGEICVAHACEEAAIEEVFVNVNPSSTLKLGHARAAV
                     MLAPARCGLPVAEYSPNLIKKAVVGAGHADKGQIAFMVKRLMPASCQGVGGDVKADAA
                     DALAVAITHAQLRKRALLETLRGAA"
     gene            1294..2970
                     /locus_tag="VF633_12890"
     CDS             1294..2970
                     /locus_tag="VF633_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8472002.1"
                     /translation="MSASASAVAAASMRAGKRIVHTLREAHATAPETAVVLAEGRLIQ
                     RGALRRLVQGGAVIDTGSGTYWLDEAAYVEMKKLRTSRIILSLFGAAALLIVVATVTI
                     AKADTPQAAPVRADQTVFRDLYKELVETNTTLSEGSCTLAAQRMVARMTAAGFSDADA
                     NVLIPEDQPKFGSLIATLPGTDTAAPAMLLLAHIDVVEARRADWQRDPFVLVEEDGFF
                     YARGASDDKAQASVWVDSLIRLKQQGFQPRRTIKLALTCGEETNDNWNGVQWLLEHHP
                     ETLQAAFALNEGAGGQLDANANRIALNIQAGEKVYQDFMLELTNPGGHSARPRKDNAI
                     GAMGAALDRLVKHDFPLELSPVTRAYFAAIAETTPASAADLRALTANEAPDTAAAARL
                     AAANPVWNAMMRTTCIPTLINGGHAPNAQPQKVTVNINCRILPGHTLAETQEEIAGAL
                     ANPAITIATTGEPSPTSPPPPLTPAILDPVKTVAAKIWPGVPVVPTLSTGATDGQFTN
                     AAGIPTYGVTGMFTDPDGNGVHGLNERLRVRSLYEGRDFLYELIQFYAMQ"
     gene            complement(3006..3524)
                     /locus_tag="VF633_12895"
     CDS             complement(3006..3524)
                     /locus_tag="VF633_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8472003.1"
                     /translation="MDITQLILDDHAEQRRLFAIIEQIGPDDVDALTKVWSRLSAFLD
                     VHAEAEERFFYPQLLKRGEGANDAEDGTVEGETEDAIEDHNKLRDAVAAANRLPIGTG
                     AWIEAVGKANVVNSKHMGEEERQGLTDFRRHAPIQLRHDLAVQFAAFESDHLLGIKPV
                     NKDPEAYIEQHG"
     gene            complement(3595..>3797)
                     /locus_tag="VF633_12900"
     CDS             complement(3595..>3797)
                     /locus_tag="VF633_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472004.1"
                     /translation="KLIADQEDHHSKAEGWMHQITTLRAELEGARAEAAMSSAALDTA
                     RRERSGRPVVTETHGSLQSIVG"
BASE COUNT          668 a         1328 c         1189 g          612 t
ORIGIN      
        1 gactcccccg tcaccggccc aactcctgca gcgcagcgcg gatgacggcg ggtagctcgg
       61 catcttcacc cagacggatc agcgcctgat cgacggcccg acgggcgttg atctcggcaa
      121 tgcccagacc cagcagggcc gagacagcct ccccggacac gctcgggacc ggggccgcga
      181 tctcggtgtg gacgcccggc gcattcggcg tgaacgagat atcacccagc gacttgccct
      241 tcagctcagt gacaatgcgc aacgccagct tgggaccgac gccgttggcg cgacccacgg
      301 ccgccttgtc gtcacgcgcc acggcgcccg ccagttcgcc cggcggcaga acgtcgagca
      361 ccgacagcgc ggccttgggt ccgacgccct gaatggccag cagcgtcgta aacgcccggc
      421 gctcgtcacg ggtcaggaag ccatacaggc gagggccccc gtcctcgctc cactggctgt
      481 gaacatgcag ggtggcctcg tcgcccggcg cgggaagtcg tcccaacgtt cgcgccccac
      541 aggcgacgac atagccgaca ccggcgcagt cgatcaggca atggccctcc tcgatctcgg
      601 ccagaacgcc tctcaaacga ccaatcatgc cgcgcctcgc agggtttcga gcagcgcccg
      661 cttgcgcagt tgggcatggg tgatcgcaac cgccaacgca tcggcggcgt cagccttcac
      721 gtcgccgccc accccttggc acgaggccgg catcaaccgc ttcaccatga aggcgatctg
      781 ccctttgtcg gcatgcccgg cgcccaccac ggccttcttg atcaggttcg gcgaatattc
      841 ggccaccggc aggccacatc gggccggcgc cagcatgacg gcggcgcgcg catggcccag
      901 cttcaaggtc gaggacggat tgacgttgac gaacacctcc tcgatcgcgg cctcttcgca
      961 cgcgtgcgcc acgcagatct cgcctaccgc ctcgagcaga tgcagcagcc gctccgagaa
     1021 cggcgcacgc tcgtcaggcg tgaccacgcc atgagcgatc cagcgcaggc gcgagccctc
     1081 ggacactatc actccccacc ccgtccggcg caggccgggg tcgagtccga ggatgcggac
     1141 ggggccacca atgggaccat tctgaatcat catgttcggc attcgttctg ttctgcccga
     1201 aacccggtta ccgaacaatg accagacgcc cgatccgcat tggtgttggt ccgtggaccc
     1261 gctaatgtcc gcgcgaaacg ggagacgggg atcatgagcg caagcgcaag tgcagtagcg
     1321 gcggcatcga tgcgggccgg caagcggatc gtccatacgc tgcgcgaggc tcatgcgaca
     1381 gcaccggaaa cggctgtggt gttggctgaa ggtcgactga tccagcgcgg agccctccgt
     1441 cggctggtcc agggcggggc ggtgatcgac accggttccg ggacctattg gctcgacgaa
     1501 gccgcctatg tcgagatgaa gaagctgcgc accagccgga tcatcctgtc gctgttcggc
     1561 gcagccgccc tgctgatcgt cgtcgccacc gtgaccatcg ccaaggccga tacgccgcaa
     1621 gccgctccgg tccgagccga ccagacggtc ttccgcgatc tgtacaagga actggtcgag
     1681 accaacacga ccctgtccga aggcagctgc acccttgctg cccagcgaat ggtggcgcgg
     1741 atgaccgccg ccggtttctc ggatgccgac gccaacgtcc tgatccccga ggaccagccg
     1801 aagttcggct ctctgatcgc caccctgccc ggcacggaca ccgccgcgcc cgccatgctg
     1861 ttgctggccc atatcgatgt ggtcgaggcc cggcgtgccg actggcagcg tgatcctttc
     1921 gtcctcgtcg aggaggacgg cttcttctat gcgcgcggcg cttcggacga caaggcgcag
     1981 gcttcagtct gggtcgacag cctgatccgc ctgaagcagc agggtttcca gccacgtcgc
     2041 accatcaagc tcgccctgac ctgtggcgag gaaacaaacg acaactggaa cggcgtccag
     2101 tggttgctgg agcaccaccc cgagaccctg caggcggcgt tcgccctgaa cgagggcgcg
     2161 ggcggacagc tcgacgccaa tgccaaccgt atcgcgctca acatccaggc tggcgagaag
     2221 gtctatcagg acttcatgct ggagctgacc aacccgggcg gtcactcggc gcgcccacgg
     2281 aaagacaacg ccatcggcgc catgggcgca gcgctggatc ggctggtgaa gcacgacttc
     2341 ccgctggaac tgtcaccggt gacgcgcgcc tatttcgctg cgatcgcaga gaccacgccc
     2401 gccagcgccg ccgacctgcg cgccctgacg gctaacgagg cgcccgacac cgccgccgcc
     2461 gcgcgtctgg ccgcagccaa cccggtctgg aatgctatga tgcggacgac ctgtattccg
     2521 acgctgatca acggcgggca cgcgccgaac gcccagccgc agaaggtcac ggtcaacatc
     2581 aactgccgca tcctgccggg ccataccctc gcggaaactc aagaggaaat cgccggtgca
     2641 ttggctaacc cggccatcac cattgcgacc accggcgagc ccagcccgac ctcgccgccg
     2701 ccaccgctga cgcccgcaat cctggatccg gtgaagacgg ttgcggcgaa gatctggccc
     2761 ggtgttccgg tcgtgccgac cctctcgacc ggcgccaccg acggacagtt caccaacgcc
     2821 gcggggatcc ccacctatgg cgtcaccggc atgttcaccg atccggacgg caacggggtc
     2881 catggcctga acgagcgact gcgcgtccgc tcgctctacg aaggccggga cttcctgtat
     2941 gaactcatcc agttctatgc catgcagtag caagagccgg ggcaatagcg agagccagcg
     3001 cggcctcagc cgtgctgttc gatataggcc tcggggtcct tgttgaccgg cttgatgccc
     3061 aacaggtgat cggactcgaa cgcagcgaac tggacagcca gatcgtggcg aagctggatc
     3121 ggggcatgac ggcggaagtc ggtcagaccc tgacgctctt cttcgcccat atgtttggag
     3181 ttgacgacgt tggccttgcc gacagcctcg atccaggcgc cggtgccgat cggcagcctg
     3241 ttggccgccg cgaccgcatc gcgcagcttg ttatggtcct cgatagcgtc ttcggtctcg
     3301 ccttcgaccg tgccgtcctc ggcgtcgttg gcgccctcgc cgcgcttcag cagttgcgga
     3361 tagaagaacc gctcttcggc ctcggcgtga acatccagaa acgcggacag ccggctccag
     3421 accttggtca gggcgtccac gtcgtcaggg ccgatctgct cgatgatcgc gaacagcctg
     3481 cgctgttcag catgatcgtc gagaatcagc tgggtgatgt ccatggggag gtcctttgat
     3541 ctgagcccgg gcttaacccg ggatcgggcc cacggttccg cttcgcgtcc cttgtcagcc
     3601 cacgatggac tgcaaggagc cgtgggtctc ggtcaccacc gggcggccgc tacgctcgcg
     3661 gcgagccgtg tccagagccg cagacgacat cgccgcttcg gcgcgggcgc cttccagctc
     3721 agcgcgaagc gtcgtgatct ggtgcatcca gccctcggcc ttggagtggt ggtcttcctg
     3781 atcggcgatc agcttgg
//
LOCUS       DASVTM010000467         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_575945,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000467 DASVTM010000000
VERSION     DASVTM010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575945"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..850
                     /locus_tag="VF633_12905"
     CDS             161..850
                     /locus_tag="VF633_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX8472005.1"
                     /translation="MSHVVVTKGEGQVIKTGVSKRWKIAVAVLALLGIGAWSGGLLNP
                     RAIAQALKDPLSLFAARSPGERAPGALTQKKPPLSSGVQIARVVPRERVLSSGRSRPA
                     GPVETEAPGTPGMTIAEPVDGDLAGTPLFSDSGIVAGGGGGGGGGAPFFVPPTSNGPG
                     VGGGGEPPVGGGPLVPPTSPVPEPETWLTMMIGLFMIGSVLRRRKIGAAASLSVPLGT
                     PGDDVTRAIRG"
     gene            861..1502
                     /locus_tag="VF633_12910"
     CDS             861..1502
                     /locus_tag="VF633_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8472006.1"
                     /translation="MRFLILFGLATATLATAGVVVRENGVAQGSEVVTLPAGPEARIR
                     YPAGALPVLTTADGKRRPVRSVLNVGRQMNYGDYVWDDANIPEGPAWVRIDLTTQTLS
                     LFRAGHEIGTAVILYGTDNKPTPTGVFPILQRAEKHRSSLYDAEMPYMLRLTHDGVAI
                     HASSVRRGSATHGCIGVPREFARRMFAQVRRGSIVSIERSGTSTSGRAGETTA"
     gene            1610..2134
                     /locus_tag="VF633_12915"
     CDS             1610..2134
                     /locus_tag="VF633_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8472007.1"
                     /translation="MISSSKASPIAACVLALTAPFLAGATGLTSNFEQRVLAAHNRER
                     TAASIRPLQWDSGLAASAQAWADRLAATGAFEHAPERAADPQGENLWAGTKGFFSAEA
                     MVDGWIREKRYFKPGAFPHNSVTGKVADVGHYTQLMWRDTGEVGCAIATGPVEDVMVC
                     RYAQAGNYLGEQPF"
     gene            complement(2255..2389)
                     /locus_tag="VF633_12920"
     CDS             complement(2255..2389)
                     /locus_tag="VF633_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472008.1"
                     /translation="MRQMAGDCDVFLEASTISWKDIAAAMLMSSGLWVAIIGACNAIV
                     "
BASE COUNT          589 a         1014 c         1076 g          611 t
ORIGIN      
        1 ataggctcga cggcgtacgt atcagcgcct acgcaggacc attgccgtcg agaaagcggg
       61 ccgatgcaac gagaccgcat ctgacccgct ggggacggta gcgatccgag gatgcaaccg
      121 ttttgtctgc ggaacgttat atctgaaggg agtgccaccg atgtcacatg tcgtggtcac
      181 caaaggggag gggcaggtca tcaagacggg cgtgagcaag cgatggaaga ttgcggtcgc
      241 ggtgctggcg ctactgggca tcggcgcctg gtcgggcggc ttgctcaacc cgcgtgccat
      301 tgcccaggcc ttgaaggatc cgctctcctt gttcgccgcg cgatcgcctg gcgagcgcgc
      361 gccgggcgcc ctgacccaga aaaagccgcc acttagttca ggcgtgcaga ttgcgcgagt
      421 cgttccgcgc gaacgagtgt tgtcgagcgg ccggtcgcga ccggccggcc ccgtggagac
      481 cgaagcgcct ggaacgccgg gtatgacgat cgccgaaccg gtcgacgggg atttggccgg
      541 aacgcccttg ttcagcgatt cggggatcgt tgctggtgga ggcggaggcg gtggaggcgg
      601 tgcgccgttc ttcgttccgc cgacctcgaa cgggcccggc gtcggcggcg gcggtgagcc
      661 tcccgtcggg ggcgggccct tggtccctcc aacgtccccg gtccctgagc cagagacctg
      721 gctgacgatg atgatcggac tgttcatgat cggctccgtg ttgcgccgga ggaagatcgg
      781 ggcagcggcc agcctgagcg tccccctggg gacgcctggc gacgatgtaa ctcgcgctat
      841 tcgcggctga ggtcggtcgg atgcggttcc tgatactctt tggcctggcg acggcgacac
      901 tggcgactgc cggcgtcgtc gttcgcgaga atggcgtcgc gcagggcagc gaggtggtca
      961 ctctcccggc agggcccgag gcgcgcatcc gctatcccgc tggcgccctg cccgtgctca
     1021 ccaccgccga cggcaagcgg cgcccggtcc gcagcgttct caatgtcggc cgacagatga
     1081 attatggcga ctatgtctgg gacgacgcca acattccaga ggggccggcg tgggtgagga
     1141 tcgacctcac cacacaaacg ctgtcgctgt tccgggccgg gcatgagatc ggtactgccg
     1201 tcattctcta cggaaccgac aacaagccga caccgaccgg agtcttcccg atccttcaac
     1261 gagcggaaaa gcatcgctcg tcgctctatg acgccgagat gccctacatg ctgcgcctga
     1321 cgcacgatgg tgtcgcaatc catgccagca gtgttcgccg gggttcggcg acgcacggct
     1381 gcatcggcgt gccaagggaa tttgcccgca ggatgttcgc gcaggtccgg cgcggctcga
     1441 tcgtctcgat cgagcggtcg gggacatcga ccagcggtcg ggctggggag acgacggcct
     1501 gacgaagagc gcggcggcga tcgttgtcgc gctattaacc ccaccgctaa accgaccgta
     1561 gagagcatca tgagacaccg ttccgcgaac agcggaaggt gtcacggcga tgatcagcag
     1621 ttcgaaggcg tcaccgatcg ccgcctgcgt cctcgcgctc accgccccgt ttctggcggg
     1681 ggcgaccggg ctgacctcca acttcgaaca gcgggtgctg gcggcgcata atcgcgaacg
     1741 gacggcagcc agcatccgcc cgctgcaatg ggactccggc ctcgcggcgt cggcccaggc
     1801 gtgggccgat cgcctggcgg cgacaggcgc cttcgaacat gctcccgaac gcgccgccga
     1861 tccccaggga gaaaatctct gggccggcac caagggcttt ttttccgcag aggcgatggt
     1921 ggacggctgg atccgcgaga aacgctattt caagccaggc gcctttccgc acaacagcgt
     1981 caccggcaag gttgccgacg ttggccatta tacccagctg atgtggcgcg acaccggcga
     2041 ggtcgggtgc gcgatcgcga ccgggccggt cgaggatgtc atggtctgtc gctatgccca
     2101 ggcgggcaac tatctgggtg agcagccctt ctagccaggg agcacgctgt cggtctgttc
     2161 ggacgacggc gcgctgccgc ggatgcgcca acatcgcggc ccgatcatcc agcaatgggc
     2221 cgaacgagcg ggctccgagc cagcgtgggc cgggtcaaac gatggcgttg caggccccga
     2281 tgatggcaac ccaaagcccg gacgacatca gcatggcggc ggctatgtct ttccagctga
     2341 tcgtcgacgc ttcgaggaac acgtcgcaat ccccagccat ctgcctcatc tcacctctcc
     2401 catctgcccc ctggggttct gattttgctt aacagcattg attaacaccg tcgtttcact
     2461 ccgcgaatcc catgtcaacg gcataatatc gaaacacgcc cagttgatgg gtaacccgtc
     2521 gcctcgagcg cgagttccgt cggcgttcgc gcctctggtc atcctcgttc gtcgcgcagt
     2581 ggggtcgcga accaaggccg gtcgcattgc ctggccagcc gatcgatcgt cttgcgacaa
     2641 gggtgtggtg cgaggtctta cgggcgcgcc tcctacggcg gcggcaggtc tgagaccctg
     2701 actggctgat cggcgcgccg acgggtggtt ggcaggctgg cgggcggatg atcgagcgac
     2761 ggagcagtct ggattccggc gctcggcggg atcaaccttc cccaacgcaa gcaacttgat
     2821 gagatggacc cacacagggt gcgcgtcatg cgatttagcg tcgccgagtg cggtcccgaa
     2881 ctggcacatt ctacctaaca tagctcaggg gagaagcaca cccgactcta aaatgaatgt
     2941 ttaggtccaa gtccatgctg ttgatattgc tggacgtttt tttcgctcaa actcctctaa
     3001 atctcgcttc agctgcgatt ggcgtctcgc tgcagtgcgc aaaaactctg gactcatagt
     3061 tgcgaatcgg ttaattaatt tctcggaaga aaagttgtca tcaaccgctc ggcgtaatgc
     3121 cgacgaccga acggggagtt tattatgcgg aaattcttga tctcaagtgc tgtggtggct
     3181 gctgcgtttg ccacgccggc agcggcgcag tcgatcacct tcgtcaatgg caactacgag
     3241 ttgccgggct tcacgactgc ggcgattttt gaaaccttcg agggtggaat
//
LOCUS       DASVTM010000468         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_311810,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000468 DASVTM010000000
VERSION     DASVTM010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311810"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VF633_12925"
     CDS             <1..979
                     /locus_tag="VF633_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEX8472009.1"
                     /translation="EAYPDFDIEAVTAHGRSKGVQLIGHHETGGNAFHYEQQLEPAMA
                     LYERLGVHSVKTGYVADAQGTRVAGPDGSMVMAWHESQAMAQHHMKVVETGARHKVAV
                     NAHEPFKDTGLRRTYPNMISREGARGMEYAAWGQPGNPPEHEPNLVFTRLLAGPMDYT
                     PGIFGMETRSPGGVQTTWAKQLALYVVIYSPIQMAADLLVNYEANPGPFQFIKDVPCD
                     WHETRVLNGEVGDFVTIARKDRNSDVWALGAITDENPRVLNASLDFLDDGRRYRAEIY
                     RDGPNADFKGKREDIIIEQREVTSGDTLTLALAPGGGQAIRFVPLGRKR"
     gene            1092..2585
                     /locus_tag="VF633_12930"
     CDS             1092..2585
                     /locus_tag="VF633_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8472010.1"
                     /translation="MRPRLTPQAIVNMCVGFFGIQIGFGLQNVNTSRIFQTLGAEVDS
                     LAILWIAAPMTGLLIQPLIGHLSDKTWGRLGRRRPYFLFGAILTTMALIAMPNSPSLW
                     FAALMLWVMDASINITMEPFRAFVGDNLNEDQRTAGYAMQSVFIGAGAVFASVLPWVL
                     SHVFAVASVAPEGVVPDSVRIAFYVGAASLLAAVLWTVFTTREYSPEQIAAFDAAAAV
                     SSTTDAEAPARSVRAYMIGGLIWLAVGAAGFIGVAIAGIEKELYVLFGGLAVFGVLQI
                     IVGLMHRARASNGLSEILDDIFRMPATMRDLAIVQFFSWFALFAMWIYTTPAVTAVHF
                     GAASPTSAAYGEGADWVGVLMGVYNGVAALAAFLIPVIAKRTGRRGAHALNLALGGLG
                     LIGIFLIRDPALLWLPMIGVGFAWASIVSMPYAILSGAVPGRKMGVYMGIFNIFIVVP
                     QLVAATLLGLILKSVFGGQAIWALVIGGVSFFIAAAAALRVKEAGKA"
     gene            2662..5076
                     /locus_tag="VF633_12935"
     CDS             2662..5076
                     /locus_tag="VF633_12935"
                     /EC_number="3.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004339 - glucan 1,4-alpha-glucosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009251 - glucan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan 1,4-alpha-glucosidase"
                     /protein_id="HEX8472011.1"
                     /translation="MTLIQRLTASAAVAALLATGACASMPAAPTAPSVQTEAVAPGAP
                     GTTTTWSSAAKTGVGASYEAYVDGQYKDGGATGAVSRVWFSIADGVLTETMFGLIHEA
                     QIKSLRFALASDAGLSLEGTDTTEHTAYLNSDAQGRPLSPAYRVVTTDKAGRYEIEKR
                     IFTDPDRNALVLRVTIKATGAAVTPYAILEPHMANTGGGDFASADANGLYAHEGDVHL
                     ALRPSAPFDTASAGFLGASDGLTDLKANGRLTHAYASTGDTAGAVVLTGALPTIAAGQ
                     SLTRDFVIGFGASKAAAETAADGSFSTGLDEVLARFNGTDARVGWEDWIVSLTDLPRI
                     AEQSTDGGKLAYASALMLKTQEDRTHAGALIASLSNPWGDTVDATVSSTGYKAVWPRD
                     FYQVAMALLALGDNQTPVAAFRYLPQVQVKADTPGNTGATGWFLQKTHVDGEVEWVGV
                     QLDQTAMPIMLGWRLWKAGLVSDAELQTMYGRMIKPAADFLVTGGKIGILWNDRTVTP
                     PFTQQERWEEQDGYSPSTTAAVIAGLTVAADIAKASNDTASATRYLAAADDYSKKADA
                     RMFTTDGALGDGDYFMRLSKTEDPNSHAKMGDNNGQPGQAQDRVVDGGFLELVRYGVR
                     SANDPHILATLPEYDDQTREDLTRVRYDFGPAGDTTPGWRRYGIDGYGETTDRGLNYG
                     QGGSGDQQDPAQRGRVWPIFTGERGHYELAHALQSGGSAADLTAIRQTYVRGMERFAN
                     DGLLLSEQVWDGVGVKPANYETGAGTDSATPLAWSHAEYIKLLRSLADKAVWDRYAPV
                     AERYSK"
     gene            complement(5117..>5562)
                     /locus_tag="VF633_12940"
     CDS             complement(5117..>5562)
                     /locus_tag="VF633_12940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8472012.1"
                     /translation="GGRILWVGCRAYTADDYAVLEAGGGEVWTTDIDPDAERWGVADR
                     HRTGDVCVIDQVFGDMTFDAISCNGVLGYGVDTPEQQRQALAAMANILRPGGRLLLGW
                     NTDKIDDPIAAGLTAPEFTPTPYAGQPSRVQFDTVTHVYDALIRT"
BASE COUNT          912 a         1971 c         1728 g          951 t
ORIGIN      
        1 cgaagcctat cccgattttg acatcgaagc ggtcactgcc cacggccgtt cgaagggcgt
       61 ccagctcatc ggtcaccacg aaaccggcgg caacgccttc cactacgaac aacaactcga
      121 accggcgatg gcgctgtacg agcgtctcgg cgtccactcg gtcaagaccg gctatgtcgc
      181 ggatgcccaa ggcacgcgtg tcgctgggcc cgacggatcg atggtcatgg cgtggcatga
      241 gagccaggcg atggcccaac accacatgaa agtcgtcgag accggcgctc ggcacaaggt
      301 ggcggtcaac gcccacgagc cgttcaagga caccggcctg cgccgcacct atcccaacat
      361 gatcagccgc gaaggcgcgc gggggatgga atatgcggcc tggggtcaac ccggcaaccc
      421 gcccgagcat gagcccaatc tggtcttcac ccgcctgctg gccggcccga tggactatac
      481 gcccggcatc ttcgggatgg agacacgcag ccccggcggc gtgcagacga cctgggccaa
      541 gcaactggcg ctctatgtcg tcatctacag cccgatccag atggccgccg acctgctggt
      601 caactacgag gccaatcccg gcccgttcca gttcatcaag gacgtgccgt gcgactggca
      661 cgagacccgg gtgctgaacg gcgaggtcgg cgacttcgtc accatcgccc gcaaggaccg
      721 caactccgat gtctgggctc tgggcgccat caccgacgag aacccgcgcg tcctgaacgc
      781 ctctctcgac ttcctcgacg acggccgccg ctaccgtgcc gaaatctatc gcgacggccc
      841 gaacgccgac ttcaagggca agcgtgagga catcatcatc gaacagcgcg aagtgacgtc
      901 gggcgacacc ctgacgctgg ccctcgcgcc cggcggcggt caggcgatcc gcttcgtacc
      961 gctgggcagg aagcggtgaa tatggctact cgcgcgtatt tatcgtcgtc gaattcaccg
     1021 ctgatcttgt cccttaattc tccggcgagt ctctcatgac gtcccccaca ggatcgcccg
     1081 gcctgttgtc gatgcgcccg cggcttacgc cgcaggccat cgtcaacatg tgcgtcggtt
     1141 tcttcggcat ccagatcgga ttcggcctcc agaacgtcaa caccagccgc atattccaga
     1201 cgctgggcgc cgaggtcgac agtctggcca tcctgtggat cgccgcgccg atgacggggc
     1261 tgctgatcca gcccctgatc ggtcacctca gcgacaagac ctgggggcgt ctgggtcggc
     1321 gtcggcccta tttcctgttc ggcgcgatcc tgacgacgat ggccctgatc gccatgccga
     1381 actcgccgag cctgtggttc gcagccctga tgctgtgggt catggacgcc tcgatcaaca
     1441 tcacgatgga gccgttccgc gccttcgtcg gcgacaatct gaacgaggac cagcgcaccg
     1501 ccggctatgc gatgcagagc gtcttcatcg gcgccggggc cgtgttcgcc tcggtcctgc
     1561 cgtgggtgct ttcgcacgtg ttcgcggtcg cctcggtagc gcctgaaggc gtcgtccccg
     1621 acagcgtccg catcgccttc tacgtcggcg cagcctcgct gctggccgcc gttctctgga
     1681 ccgtcttcac cacgcgcgaa tacagccccg agcagatcgc cgctttcgac gccgctgccg
     1741 ccgtatcctc tacgaccgac gctgaggctc cggcccggtc ggtgcgcgcc tatatgatcg
     1801 gaggcctgat ctggctcgcg gtcggggctg cgggcttcat cggcgtcgcc attgccggga
     1861 tcgagaagga actctacgtc ctgttcggcg gcctcgccgt cttcggcgtg ctgcagatca
     1921 tcgtcggcct gatgcatcgc gcccgagcat cgaacggcct gtccgagatc ctcgacgaca
     1981 tcttccgcat gccggccact atgcgcgatc tggccatcgt ccagttcttt agctggttcg
     2041 ccctgttcgc catgtggatc tacacgaccc ccgccgtcac cgccgtccat ttcggcgccg
     2101 cctcccctac ctccgccgcc tatggcgagg gcgccgactg ggtcggcgta ttgatgggcg
     2161 tctacaacgg cgtcgcggcg ctggcggcgt tcctgatccc cgtcatcgcc aagcgaaccg
     2221 gtcggcgcgg cgctcacgcc ctcaacctcg cgctcggcgg gctgggcctg atcggcatct
     2281 tcctgatccg cgatccggcc ctgctgtggc tgccgatgat cggtgtggga ttcgcctggg
     2341 cttcgatcgt gtcgatgccc tacgccatcc tttcgggagc ggtgccgggg cgcaagatgg
     2401 gggtctatat gggcatcttc aacatcttca tcgtcgtgcc ccagctggtc gccgcgaccc
     2461 tgctgggtct gatcctgaag tcggtgttcg gcggtcaggc catatgggct ctggtcatcg
     2521 gcggcgtgtc cttcttcatc gccgccgcag ccgccctgcg cgtcaaggaa gcgggcaagg
     2581 cctgatgcgt ccccaacttc acattctgaa attcctcctg tcgccatcct ccgcggcgat
     2641 cgcatcgtga gtatcctgac catgaccctg atccagcgcc ttaccgcatc cgccgccgtc
     2701 gccgcccttc tggccactgg cgcctgcgcc tcgatgcccg cggctccgac agccccttct
     2761 gttcagaccg aagccgtcgc acccggtgcg cccggcacga cgacgacatg gtccagcgcc
     2821 gccaagaccg gcgtcggcgc gtcctacgag gcctatgtcg acggccagta caaggatggc
     2881 ggcgcgaccg gcgccgtgtc gcgcgtatgg ttctccatcg ccgacggtgt cctgaccgaa
     2941 accatgttcg gcctgatcca tgaggctcag atcaagtcgc tgcggttcgc gctcgcgtcc
     3001 gacgcgggcc tgtcgcttga gggaaccgac accaccgaac acaccgcata cctgaactcc
     3061 gacgctcagg gccgcccgct gtcgcccgcc tatcgcgtgg tcaccaccga caaggccggt
     3121 cgctacgaga tcgaaaagcg catcttcacc gacccggacc gcaacgcgct cgttttgcgc
     3181 gtcacgatca aggcgaccgg cgcagccgtg acgccctacg ccatcctcga gccgcatatg
     3241 gccaacacgg gcggcggcga cttcgcctcg gccgacgcca acggcctgta tgcccatgag
     3301 ggcgacgtcc atctggcctt gcgcccgagc gcgccgttcg acaccgccag cgcgggcttc
     3361 ctcggcgcct cggatggctt gaccgacctc aaggccaacg gtcgcctgac ccacgcctac
     3421 gcctcaaccg gcgacaccgc cggcgccgtc gtcctgaccg gcgccctgcc gaccatcgcg
     3481 gcgggccaga gcctgacgcg cgacttcgtc atcggcttcg gcgcgtcgaa ggcggctgcc
     3541 gagacggcgg ccgacggcag tttctcgacc ggtcttgacg aggttctggc ccggttcaac
     3601 ggcacggacg cgcgcgtcgg ctgggaggac tggatcgtct ctctgaccga cctgccgcgc
     3661 atcgccgaac agtccaccga cggcggcaag ctggcctatg cctcggccct gatgctgaag
     3721 acgcaggagg accgcaccca cgcgggcgcg ctgatcgcat cgctgtccaa cccgtggggc
     3781 gacacggtcg acgcgaccgt aagctcgacc ggttacaagg ccgtctggcc gcgcgacttc
     3841 tatcaggtgg ccatggcgct gctggcgctg ggcgacaatc agacgccggt cgccgccttc
     3901 cgctacctgc cacaggttca ggtcaaggcc gacaccccgg gcaatacggg cgccaccggc
     3961 tggttcctgc agaagaccca cgtcgacggc gaggtcgaat gggtcggtgt ccagctcgat
     4021 cagacggcca tgccgatcat gctcggctgg cgtctgtgga aggcgggcct cgtgtccgac
     4081 gccgaactcc agaccatgta cggccggatg ataaagcccg cagcggactt cctcgtcacc
     4141 ggcggcaaga tcggcatcct gtggaatgat cgcaccgtga cgccgccgtt cacccagcag
     4201 gagcgctggg aggaacagga cggctattcg ccttcgacca cggccgccgt tatcgccgga
     4261 ctaaccgtcg ctgccgacat cgccaaggct tcgaacgaca cggcctcggc aacccgctat
     4321 ctcgccgccg ccgacgacta ttcaaagaag gccgacgcgc ggatgttcac caccgacggc
     4381 gcgctggggg acggcgacta tttcatgcgc ctgtccaaga ccgaggatcc gaacagccac
     4441 gcaaagatgg gcgacaacaa tggccagccg ggccaggccc aggaccgcgt cgtcgacggc
     4501 ggcttcctcg aactggtgcg ctacggcgtc cgctcggcca acgatccgca catcctcgcg
     4561 accctgcccg agtatgacga tcagacccgg gaggacctga cccgcgtgcg ctacgatttc
     4621 ggtcctgccg gggacaccac gcccggctgg cgtcgctacg gtatcgacgg ttatggcgaa
     4681 accaccgacc ggggcctgaa ctatggtcag ggcggcagcg gcgatcagca ggatccggcc
     4741 cagcgcggcc gtgtctggcc gatcttcacc ggcgaacgcg gccactacga actggctcat
     4801 gcgctgcagt ctggcggctc agcagccgac ctgacggcca tccgccagac ctatgttcga
     4861 ggcatggagc ggttcgcaaa cgacggcctg ctgttgtccg aacaggtctg ggatggggtt
     4921 ggggtcaagc ccgctaacta tgagaccggc gcaggcaccg actcggcgac gccgctggcg
     4981 tggtcgcacg cggaatacat caagctgctg cgctcgctgg ccgacaaggc cgtctgggat
     5041 cgctacgcgc cggtcgccga acgctacagc aaataacgat caggaggttt gtcgggcggt
     5101 gccggcgaac ctccggtcag gtccggatca gggcgtcgta gacgtgagtg accgtgtcga
     5161 actgcacccg gctcggctgc ccggcatagg gggtcggcgt gaactccggc gcggtcagtc
     5221 cggctgcgat cgggtcgtcg atcttgtcgg tgttccagcc cagcagcagc cttccgcccg
     5281 gtcgcaggat gttcgccatg gccgccagcg cctgacgctg ttgttccggc gtgtcgacgc
     5341 catagcccag cacgccattg cagctgatgg cgtcgaacgt catgtcgccg aacacctgat
     5401 cgatgacgca gacgtcgccc gtcctgtgcc ggtccgccac gccccaacgc tcggcgtcgg
     5461 gatcaatgtc agtcgtccag acttcgccgc cccccgcctc aagcaccgcg tagtcgtcag
     5521 ccgtataggc tcggcacccg acccaaagga tacgcccgcc ct
//
LOCUS       DASVTM010000469         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1000312,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000469 DASVTM010000000
VERSION     DASVTM010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000312"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VF633_12945"
     CDS             <1..794
                     /locus_tag="VF633_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8472013.1"
                     /translation="SEDEYRLVERRPLARTGDWTSHPEVRDAESAIRSTKRLVALARE
                     TGARIHVLHVTTAEEIAFLADNKDVATVEVTPQHLTLDGDEAYARLKGLAQMNPPIRN
                     APHIAGLWRGIEHGVVDVLGSDHAPHTLEEKARPYPASPSGMPGVQTLVPVMLTHVAN
                     GKLSLERFVDLTSAGANRVFGTAGKGRMAEGYDADLTIVDLKKQKTITHAEMASRSGW
                     TPFDGFEATGWPIATIIRGRTVMRDGQLIGTAHGRPVRFQETLGS"
     gene            791..1540
                     /locus_tag="VF633_12950"
     CDS             791..1540
                     /locus_tag="VF633_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX8472014.1"
                     /translation="MTETARTKLGLIGFGAFGRLIARHLSDPFDILVHDPAATENEGR
                     VTLTDLASAAACPIVILAVPVEAMEAAVAAIAPHVTTGAVIIDVGSVKVLPARLMAEG
                     LPGNVRIVGAHPLFGPQSGKDGIAGLRIAVCPINDARMARRVAAFCRSVLKLKVFLVS
                     PEDHDREAAVVQGLTHLIARLLLAMGPLPTRMTTTSFERIMQAVEMVRHDSPAVFRAI
                     ERDNPFAAEVRQKFFALADDARADLDGPSEV"
     gene            complement(1537..2472)
                     /locus_tag="VF633_12955"
     CDS             complement(1537..2472)
                     /locus_tag="VF633_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8472015.1"
                     /translation="MLTVLFAGALTLAQTAAPGSTEVALPSPPASLYGTLLTPAGATT
                     AVAVIIPGSGPTDRDGNNPLGVSAGTYRLLAEGLAEKGVATIRIDKRAIGKSAPAALD
                     ESKLSFGDYAADARAWAAEAATRAGQPCAWLIGHSEGSLVALDAVQTPDDKICGVISL
                     SGAGRPAGAVIREQLAAVPEPLKTQAFAALAELEAGRTVADTPPQLAALFRPSVQPYL
                     ISWLPLDPAKLAAAYRGPLFIGQGTTDLQISMTDAEALAAARPDATLVRFEGVNHVLK
                     TAPADQAANAATYRDPALPLAPGVVEAIAAFIADH"
     gene            2617..3375
                     /locus_tag="VF633_12960"
                     /pseudo
     CDS             2617..3375
                     /locus_tag="VF633_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
     gene            complement(3388..3789)
                     /gene="erpA"
                     /locus_tag="VF633_12965"
     CDS             complement(3388..3789)
                     /gene="erpA"
                     /locus_tag="VF633_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX8472016.1"
                     /translation="MTSVQSTTSATASEPRFEFKVATRTPEGIILSPSAAKRLAKLGV
                     AEGHPVMLRVAVEGGGCSGFQYKMDLVETAEADDLRIETDGQSLLVDPVSVPFLKGSE
                     IAYVDELAGAQFVVRNPNAATSCGCGVSFSI"
BASE COUNT          654 a         1239 c         1319 g          602 t
ORIGIN      
        1 actccgagga cgaataccgt ctggtcgagc gccggcctct ggcccgcacc ggcgactgga
       61 ccagccaccc cgaggtgcgt gacgccgaga gcgccatccg ttcgacgaag cgtctggtgg
      121 cgttggcccg ggaaaccggc gcccgcatcc acgtcctgca cgtcaccacc gccgaagaga
      181 tcgcgttcct cgccgacaac aaggatgttg ccacggtcga ggtcacgccg cagcacctga
      241 cgctggacgg tgacgaggcc tatgcgcggc tcaagggtct ggcgcagatg aatccgccca
      301 tccgaaacgc cccgcacatt gcgggcctgt ggcgcggcat agagcatggc gtcgtggacg
      361 tcctcggctc ggatcatgcg ccgcacacgc tcgaggaaaa ggcgcggccc tatccggcgt
      421 cgccgtcggg gatgccgggc gtacagacgc tggtgccggt gatgctgacc catgtggcca
      481 acggaaagct gtcgctggag cggttcgtcg acctgacttc ggctggcgca aaccgggtgt
      541 tcggcacggc aggcaagggc cggatggccg agggctatga cgccgacctg accatcgtcg
      601 acctgaagaa gcaaaagacg attacgcacg ccgagatggc gagccgctcc ggctggaccc
      661 ccttcgacgg cttcgaagcg acgggctggc cgatagccac gatcatccgc ggccgcaccg
      721 tcatgcgcga cggccagttg atcggcacgg cgcacggacg tccggtgcgg tttcaggaaa
      781 cgctcggctc gtgacagaga cggcgcgcac gaagctcggc ctcatcggct tcggcgcctt
      841 cgggcgattg atcgcgcgcc acctgtcgga cccgtttgat atcctcgtcc atgatcctgc
      901 ggcgacggag aacgaaggcc gcgtgacgct gaccgatctg gccagcgccg ccgcctgtcc
      961 gatcgtcatc cttgccgtgc ccgtcgaggc gatggaggcg gcggtcgccg ccatcgctcc
     1021 gcatgtgaca accggcgccg tgatcatcga cgtcggctcg gtgaaggtcc tgcctgcgcg
     1081 tctgatggcc gaggggttgc ccgggaatgt ccgcatcgtc ggcgcccacc ctctgttcgg
     1141 accccagagc ggcaaggacg ggattgccgg cctgaggatc gccgtctgtc cgatcaacga
     1201 cgcccgcatg gcgcgtcggg tcgccgcctt ctgccggtcg gtgctgaagc tgaaggtctt
     1261 cctcgtcagc cctgaggatc acgatcgcga ggcggcggtg gtgcaagggc tgacacacct
     1321 gatcgcgcgg ctactgctgg cgatggggcc cctgccgacg cggatgacga cgaccagctt
     1381 cgagcggatc atgcaggcgg tcgagatggt gcgccacgac agcccggcgg tgttccgcgc
     1441 catcgaacgc gacaatccct tcgccgccga ggtccgccag aagttctttg ctctagcgga
     1501 cgacgctcgc gctgatctgg atgggccgtc tgaggtctag tggtcggcga tgaaagcggc
     1561 gatggcttcg accacgcctg gggcgagcgg cagggccgga tcgcggtagg tagcggcgtt
     1621 tgcagcctga tcggccggcg cggtcttgag cacgtgattg acgccctcga accgcaccag
     1681 cgtcgcatca ggccgagcgg cggccagagc ttcggcgtcg gtcatcgaga tctgaaggtc
     1741 ggtcgtgccc tgaccaatga acagcgggcc gcgatacgca gccgccagct ttgcgggatc
     1801 cagcggcaac cacgagatca gatacggctg gaccgaggga cggaacaggg cggcgagctg
     1861 cggcggggtg tcagcaaccg tgcggccagc ctcgagttcc gccaacgcgg cgaaggcctg
     1921 cgtcttcagg ggctcgggca cagcggccag ttgctcgcgt atcaccgcgc ccgccggacg
     1981 gcccgcacca gacaaggaga tgacgccgca gatcttgtca tccggcgtct gcaccgcgtc
     2041 gagtgcgacc agcgagcctt cgctatggcc aatcaaccat gcgcagggct gacccgcgcg
     2101 cgtcgcagcc tctgctgccc acgcccgggc atcggctgca taatcgccaa agctgagttt
     2161 ggactcgtcc aaggcagcag gagcgctctt gccgatagct cgcttatcga tgcggatcgt
     2221 ggcgacgccc ttctcagcta aaccttcggc caacaggcga taggtgccag ccgagacgcc
     2281 gagcggattg ttaccgtcgc gatccgtagg gccggagccg gggatgataa cagcgacagc
     2341 cgtggtggct ccggcaggtg tcagcaaggt tccgtacagc gacgctggcg gcgacggcag
     2401 ggcgacttcg gtcgaaccgg gagcggcggt ttgggcgagc gtgagggcgc cggcgaacaa
     2461 cacggtcagc atcttcaatg tccagttaag gtcgaggtgg cgggaaacct gaacctgatt
     2521 cttgcccgcc gcaagaagcc ccgccgtccg tgcgaaagcc ttgcaacacg cggcccggtc
     2581 gggtttgaag gccgcacagt cttccaggag cccgccatga acatcagcga tcagatcgtc
     2641 ctcgtcaccg gcggcgcgcg gggcgtcggc gctgctgtgg ccaaggcctt cgctgcacag
     2701 ggcgcgcggg tggtgatcaa ctggcgggcc agtggcgaca aggcggaggc gctgtcggcg
     2761 gaactcggtg accgcgtgat cgccatccaa gcggacgtcg ccgatcgttc agcggtagag
     2821 gcgatgatcg cccgggccga gggccatttc ggcggaccgg tcaccacggt gatcaacaac
     2881 gctctggact acagcttcaa cggcgacgcc cgttcgatgc tggccgacat tggctgggcc
     2941 gagatggaga gccagttctc gaccgtggtg cgcggcgccc tgaacgtcat tcaggcggcc
     3001 gtgcccggca tgtgccgcaa cagggagggc cgggtggtca acatcggcac caacctgttc
     3061 tagaacccgg tggtgccgta tcacgactat actgccgcaa aggccgcgct gctgtcgctg
     3121 acccgcacgg cggcgggcga cctcggtcct atggggatca ccgtcaacat ggtctcgggc
     3181 ggcctgttgc ggaccacgga cgccagcgcc gcgacgccgg acgcagtgtt cgacctgatc
     3241 gccggcatga ccccgctccg cagcgtcacc acgcccgagg agttcgcgga cgccgtgctg
     3301 ttcttcgcct cgccgtggag tcgggcggtg accggtcaga acctcgtggt cgatggcggg
     3361 ctggtgcgag attaaggcga tcagcgccta gatcgagaag ctaacgccac agccgcaact
     3421 ggtggcggcg ttcggattgc gcacaacgaa ctgggccccg gcgagttcgt cgacgtaggc
     3481 gatctcggag cccttgagga aggggaccga cacgggatcg accagcagcg actgaccgtc
     3541 ggtctcgata cgcagatcgt ccgcctcagc ggtctcgacc agatccatct tgtactggaa
     3601 gcccgagcag ccgccgcctt cgacggcgac gcgcagcatc accggatgac cctcggccac
     3661 gcctagcttg gccagacgtt tggcggcgct cggcgacagg atgatgccct ccggcgtgcg
     3721 ggtggcgacc ttgaactcga acctgggctc ggaagcagtg gccgaagtcg tggattggac
     3781 gctggtcata cctgcctata tgagacgccg gggg
//
LOCUS       DASVTM010000470         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_950085,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000470 DASVTM010000000
VERSION     DASVTM010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950085"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1140
                     /locus_tag="VF633_12970"
                     /pseudo
     CDS             1..1140
                     /locus_tag="VF633_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163887.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
     gene            complement(1215..1733)
                     /locus_tag="VF633_12975"
     CDS             complement(1215..1733)
                     /locus_tag="VF633_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472017.1"
                     /translation="MNDTFVPSYARKPVRQKKVRTWMVLAPIAAVALLGGGAYMLMDN
                     GETAQPLVEPASAPTASAMAPTVPTTEAALAPMTSAATPAPIEATPAPVTRTSAPVRT
                     APLVARRESAPVRSGAAGSASAPRIETPAQPAGPQPYGATSSLNTATPAPRMANPSVG
                     APAPAITVQPLN"
     gene            complement(1928..2404)
                     /locus_tag="VF633_12980"
     CDS             complement(1928..2404)
                     /locus_tag="VF633_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HEX8472018.1"
                     /translation="MRRFSFAAALFAMAAGSVTPAAAQDYNANTNYGDLQLQAGFTPD
                     PALITLQAGGTLDAADRFDTCKGFIAEAADVRLMWSGSGKGSSPIKISVVSNADTTLV
                     VNGPDGRWYCDDDGGEDSNPSLTLTPQNGRYEIWVGTYTSGETKNSVLSISEVSSF"
     gene            2670..>3470
                     /locus_tag="VF633_12985"
     CDS             2670..>3470
                     /locus_tag="VF633_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8472019.1"
                     /translation="MGLAANIRRDLKFASGLRRLLKRIKPIELDSPVLVCDDFEEAVD
                     KFGDNIAVEDESRSVTYRELDALANRYAHWARSRNLRRSDVIGLVMTNRIEYLASWIG
                     FSKVGIATALINTNLTGQALAHCLNIVNVAQVVADEGTWKQVEEARPFAGRTLMLWVV
                     GLKSEEEASERRNLDNAVRSGSSVRPLKTVRDGLTNRDTALYIYTSGTTGLPKAARIP
                     HSRARTYMRAFAGCTNSTASDAIYNVLPLYHSTGGLVGVGPALLNGGRL"
BASE COUNT          651 a         1072 c         1133 g          614 t
ORIGIN      
        1 gcgccacgcc tgtcgtcgcg ccgggcctga aggtccaacc ccttcgcggt ggggctggat
       61 caccctcgct ggctctatcg cctgccgaac ggcgacatcc tcgtcgcgga gaccaacgct
      121 ccgcccaagc ccgagggcaa gtccgacggg atcaaggggt tcttccaggg cctgttcatg
      181 aagaaggccg gggcagtaac gcccagcccc aacaggatca tcttgctgcg ggacgcggac
      241 ggtgacggcg tcgcggagct gaaggccgag ttcctcaaga acctgatgtc gccgtttggg
      301 atggctctgg tcggcgaccg tctgtacgtc gccaacgccg atgcggtggt cagcttccca
      361 tatgtcgagg gccagacggc gatcaccgcc ggaccgacca agttgaccga cctgcccgcg
      421 cagaggaacc accattggac caagagtctc gtggccggtc cggatgggac gaagctctac
      481 gtcggggtcg gatcaaactc taacgtcgcc gactatggca tgggcgagga gatcaaccgc
      541 gccaacatcc tcgagatcga ccggatgacg ggggccaccc gggtctatgc ggcgggcctg
      601 cgtaacccgg tagggctggc ctggaacccg gagagcggca agctgtgggt cgccgtcaac
      661 gagcgtgacg agatcggcaa cgacctggtg cccgactaca tgacgtcggt gacgccgggg
      721 gcattctacg gttggcccta cagctactac ggccagaacg tggatgagcg ggtcaagccg
      781 cagaacccgg gcctcgtcgc cagcgccatc aagccggact atgcgctggg ggcgcacacg
      841 gcgtcgttgg gtctcacctt ctcggacagc acgacgctgc ctgctgcgtg gcggaatggc
      901 gcgttcgttg gtcaacatgg gtcgtggaac cgcagcacgc ccaacggcta caaggtgatc
      961 ttcgtgccat tcgccaacgg cgtgcccgcc gggacgccgc aggatgtgct gactggcttc
     1021 ctcaacgcta agggcgaggc gcagggccgc cctgtcggcg tgcagacaga tcgcaccggc
     1081 gcactgctgg tcgcggacga tgtcggcaac gtcatctggc gcgtgtcttc ggccaactga
     1141 cctcgggccg gcaaccagac atgaagaggc cccggtggat cgctccgccg gggccttctc
     1201 atacttgtta tgcgttagtt cagcggttgg acggtaatcg cgggagctgg cgcaccaacc
     1261 gacgggtttg ccatacgggg agccggtgtc gccgtgttca gcgatgaggt cgcgccgtag
     1321 ggctgcggac cagcgggctg ggctggtgtt tcgatgcgcg gagccgatgc actaccggcc
     1381 gcgccactgc ggacaggagc actttcacga cgtgccacga gcggagcggt acggaccggt
     1441 gcggaggtac gagtgaccgg ggcgggggtc gcctcgatcg gggctggcgt agcggctgac
     1501 gtcatcgggg ccagtgcggc ttctgtggtc ggaacggttg gcgccattgc cgaggctgtt
     1561 ggcgcggacg ccggctcaac aagcggctgg gcggtttcac cgttgtccat cagcatgtaa
     1621 gcgccgccgc cgagcagcgc cacggctgcg ataggcgcca gcaccatcca ggtgcggacc
     1681 ttcttctggc gcacaggctt gcgggcgtaa gatggcacaa aggtatcgtt cacgcttggt
     1741 gctacagtat catcagtata ggtgacagac atcgtttcct cctgaagttc gaggaaagaa
     1801 cagacgctgt cgatcgaggt tccgcgaaat cttcggggtc gcgaagaaac accgttgtcg
     1861 ccacattcgt gacgacaacg gggcggggct cgcctgcctg ctgaaaatca gctcgatggc
     1921 ggttgatcta gaagctggag acttcagaaa tcgaaaggac ggagttctta gtctcgccgg
     1981 aggtgtaggt gccgacccag atctcgtagc ggccattctg gggtgtaagg gtcaaggacg
     2041 ggttggagtc ctctccgccg tcgtcatcgc agtaccagcg tccgtcaggc ccattgacca
     2101 ccaaggtcgt gtccgcattg gatacaacgg aaatcttgat cggcgaactg cccttgccgc
     2161 tgccgctcca catcaggcgc acgtcggcgg cctcggcgat gaagcccttg caggtgtcga
     2221 aacgatcggc ggcatccagg gtcccgcctg cttgcaaggt gataagggcg gggtcgggtg
     2281 tgaatccggc ctgcaactgg aggtcgccgt agttggtatt ggcattatag tcttgcgccg
     2341 cagcaggcgt cacggagccc gcagccatag cgaacaaggc agcggcaaat gaaaaacggc
     2401 gcatcgaaaa ttcccccacg ctccatcacc gactattcag cgacctgcgc tgcgcgatta
     2461 aacgtcgcag gcgcttccgg atcaagtcgt agaacggaac gtttctcaaa cgtggaagcg
     2521 tctaacttga ccgtgcgtta ccggcgcatc ggcgccacgc gcagccggaa ttcatatcaa
     2581 ccgtcgttcg gatttcgcgc acgagttgac ccgggcggag gggttgtcca tcaggtgagc
     2641 ctgacggcga atatggagta acggcaatca tgggtctcgc agcgaacatt cggcgcgacc
     2701 tcaaattcgc ttctggtctc cggcgacttc tgaagcggat caaacccatc gagctcgata
     2761 gcccggtact ggtctgtgac gacttcgagg aagcggtcga caaattcggc gacaacatcg
     2821 ccgtcgagga cgagagccgg tcggtgacct atcgcgagct cgacgcgcta gccaaccgtt
     2881 atgcgcactg ggcgcgcagc cggaacctcc gccgttcgga cgtcatcggg ctggtgatga
     2941 ccaaccggat cgaatacctc gcgtcctgga tcggcttttc caaggtcggc atcgccaccg
     3001 ccttgatcaa caccaacctg acgggccagg cgctcgccca ttgcctcaac atcgtcaacg
     3061 tcgcgcaggt cgtggcggac gaaggcacat ggaagcaggt cgaggaggct cgccccttcg
     3121 ccggccgcac cctgatgctg tgggtcgtcg gactgaagtc cgaggaggaa gcctccgaac
     3181 gccgtaacct cgataacgct gtccgcagcg gttcgtccgt aagaccgttg aagaccgtgc
     3241 gtgacggcct gaccaaccgc gacacggccc tctacatcta cacctcgggc accaccggcc
     3301 ttcccaaggc tgcacggatt ccgcattcgc gcgcgcgcac ctatatgcgc gcctttgctg
     3361 gttgcacgaa ctccaccgcg tccgacgcga tctacaacgt cctgccattg taccattcga
     3421 ccggcggtct ggtcggagtc ggaccggcgc tgctgaacgg cggtcggctc
//
LOCUS       DASVTM010000471         6330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_791896,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000471 DASVTM010000000
VERSION     DASVTM010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791896"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VF633_12990"
     CDS             complement(<1..549)
                     /locus_tag="VF633_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX8472020.1"
                     /translation="MTDLLTPSAVVAFWKEAGPKLWYAKDEAFDARFRQRFEAAHWGA
                     AARRHDEWMETAEGALALMILLDQYPRNSFRGTGHQFATDALALAFAKQAVARGYLDQ
                     VEPEMRQFFLTPYEHSETLVDHDSLQDLLAGGFGAELADVARFAIIHRDIIIRFGRFP
                     HRNASLGRVTTAEEQAFLDGGGF"
     gene            548..1567
                     /gene="ilvC"
                     /locus_tag="VF633_12995"
     CDS             548..1567
                     /gene="ilvC"
                     /locus_tag="VF633_12995"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX8472021.1"
                     /translation="MNQSLPAPLPVFHDRDCDLSMICSKRVAIIGYGSQGRTHALNLR
                     DSGVAHIVVGLKAGSETRAKAEADGFTVATAGEAASGADVVAVMVSDEAHRDLYRDEL
                     EPNLQPGAALIFAHGLSVRFGLVTPRPDLDVILDSPKGIGPRIRDLYEAGEGVFCLFG
                     VHQDATGGAHALGLSYAAALGCGRKGILETTFAAECESDLFGEQVVLCGGVAELVDGA
                     FMKLVEAGYPPEVAWFECFYELKLVTDLMYERGIAGAFAKISNTAEYGAYLTGPRVIG
                     EASLKAMDGVLSDVRDGSFVKRLMADYDAGSPELLANRAALGRRTIETVGARLNEVAK
                     AANDA"
     gene            complement(1706..1782)
                     /locus_tag="VF633_13000"
     tRNA            complement(1706..1782)
                     /locus_tag="VF633_13000"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1746..1748),aa:Pro,seq:cgg)
     gene            1988..2061
                     /locus_tag="VF633_13005"
     tRNA            1988..2061
                     /locus_tag="VF633_13005"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2020..2022,aa:Gln,seq:ttg)
     gene            2323..2772
                     /locus_tag="VF633_13010"
     CDS             2323..2772
                     /locus_tag="VF633_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472022.1"
                     /translation="MELPDIRVMIVEDQVILAMDLEMVLSDAGCDVVGCALDREGAFG
                     VGERERPNLAFVDVNLLDGRTGPQIARRLVEEYGTAVVFLTANPEQIPAGFAGALGAV
                     TKPFDETTIRAVVSFAREFIFLHTVGEAPGRLQLAPWLTTQQSVAHH"
     gene            complement(2794..3336)
                     /locus_tag="VF633_13015"
     CDS             complement(2794..3336)
                     /locus_tag="VF633_13015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8472023.1"
                     /translation="MVRVRYATDADLEAVTAIYAEDVLTGTGTFELTAPGVPEMTARR
                     AAIVTLGLPWLVAEIEGVVVGYAYAGPFRTRAAYRYTVEDSIYVLASAHGRGVGKALL
                     LELIARCEAMGLRQMLGVIGDSANVGSIRLHAACGFAPMGAIEGVGWKLDDWRDVVFM
                     QRRLGPGETTSPDAPGLPFA"
     gene            complement(3348..5588)
                     /locus_tag="VF633_13020"
     CDS             complement(3348..5588)
                     /locus_tag="VF633_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8472024.1"
                     /translation="MNRRELIASSAAVALTGVASSTLAQQPGTSMSPAPVAPVPVPPV
                     AKKVPVRIEQLGRVRVDDYQWMKDDAWQQVLRDPSIIKADVKAHLTEENAYREAMMAP
                     TLPMQEAMFEEMKGRIKEDDSSVPSPDGSWEYYVEYRTGEQHPRYMRVARSGSFVNEA
                     GEAVTLNFIRAPDPELLLDTNLLAEGKAYSEVSATSNSPDHTLFAYAEDAQGSEVFRI
                     YVKDLATGEVLPEVIDSATENFTFSPDSQWIFWTNRDDNGRPDKIFRRPARGGETTLV
                     YEEADDGMFISVGVTSDDAFVLVSIGNQETSEVRYIPASDPTATPVVIEPRHAGRLYD
                     ADHWGDRWAIRTNEDAVDFRIVEAPTASPALAEWTDIVAHAPGRFIEGFGLFKGHLVR
                     QERAEANSRVIVRDRAGDEHEIAVSEPAYALSLGGSSEFDTTTMRYAYNSPSTPNQTF
                     DYDMATRERTLRKTQEVPSGHDPADYVVERLNAPAPDGQMVPITLLRKKTTPVDGSAP
                     LLLYGYGSYGNPIPASFSTNRLSLVDRGFIYAIAHIRGGSDKGWNWFLTARRHTKINT
                     FTDFIACAEHLIAEDYTTAGNIVAEGRSAGGLLMGAVTNMRPDLWAGIIGGVPFVDVI
                     NTMSDTSLPLTPPEWPEWGNPIESAADYDYMMSYSPYDNVAPMAYPAVLATGGLSDPR
                     VTYWEPEKWVAKLRPATTSGKPILLKINMTAGHFSSSGRFDYLKEIAHDYAFAVWAVN
                     KGWEAA"
     gene            complement(5848..>6330)
                     /locus_tag="VF633_13025"
     CDS             complement(5848..>6330)
                     /locus_tag="VF633_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8472025.1"
                     /translation="EGANGIQAMDLVGRKLSMDGGAAARAVIVEMKTTLASLGKFYTG
                     KPVERFKTAIEAVEDATLWLLDAKADKDRGADVLAAADAYLKLLGDVVGGWMLAKGAL
                     AAKARLDAKDGDPAWLQGKVDLYEVYAANVLGHVSSRLAAVGQGGDLLQRMSREALGA
                     "
BASE COUNT         1008 a         2168 c         2078 g         1076 t
ORIGIN      
        1 aaacccgccg ccgtccagaa acgcctgttc ctccgccgtc gtcacccgcc ccagcgatgc
       61 gttccggtgc gggaaccgcc cgaagcggat gatgatgtcg cggtgaatga tggcgaagcg
      121 ggcgacgtcg gccagttcgg cgccgaaccc gccggcaagc aggtcctgaa gcgagtcgtg
      181 atccaccagc gtctcggaat gctcatacgg cgtcaggaag aactgccgca tctccggttc
      241 gacctgatcc agatacccgc gcgccaccgc ctgtttcgcg aacgccagcg cgagcgcatc
      301 cgtggcgaac tggtgtccgg tgccgcgaaa gctgttcctc gggtactggt ccaacaggat
      361 catcagcgcc agtgcgccct cggccgtctc catccactcg tcgtgacggc gagcggcggc
      421 cccccaatgc gcagcctcga accgctgccg gaaccgggcg tcgaacgcct cgtccttggc
      481 ataccagagc ttggggcccg cttctttcca gaaggcgacg acggcggaag gcgttagaag
      541 gtcggtcatg aaccagtcct tacccgctcc gctgcccgtc ttccacgacc gcgactgcga
      601 cctgtcgatg atctgcagca agcgcgtcgc catcatcggc tatggcagcc aaggccgcac
      661 acacgcgctt aacctgcgcg attcgggcgt cgcccacatc gtcgtcgggc tgaaggctgg
      721 atcggagaca cgcgccaagg ccgaggccga cggcttcacg gtcgcgaccg ccggagaggc
      781 cgcatccggc gccgacgtcg tcgctgtcat ggtctccgac gaggcccacc gcgacctcta
      841 ccgcgacgag ttggagccca acctccagcc cggcgccgcc ctgattttcg cccacgggct
      901 gtcggtgcga ttcggtctgg tgacgccgcg ccccgacctc gacgtcatcc ttgattcgcc
      961 caagggcatc ggcccgcgca tccgcgacct gtacgaggcc ggggagggcg tcttctgcct
     1021 gttcggcgtg catcaggatg cgaccggcgg agcccacgcg ctcggcctgt cctatgccgc
     1081 ggcgctcggc tgcggtcgca agggcatcct cgagaccacc ttcgccgccg aatgcgaatc
     1141 ggacctgttc ggtgagcagg tggtcctgtg cggcggcgtc gccgaactgg tcgacggggc
     1201 cttcatgaag ctggtcgagg ccggctatcc gcccgaggtc gcctggttcg aatgcttcta
     1261 cgagctcaag ctggtcaccg acctgatgta cgagcgcggc atcgccggcg ccttcgcaaa
     1321 gatctccaac accgccgaat acggcgccta tctgaccggt ccgcgcgtca tcggcgaggc
     1381 gtctttgaag gccatggacg gcgtcctgtc cgacgtccgc gacggctcgt tcgtcaaacg
     1441 cctgatggcc gactacgacg ccggatcgcc ggaactgctg gccaatcgcg ccgccctcgg
     1501 tcgccgcacc atcgagaccg tcggcgcccg cctgaacgag gtcgccaaag ccgccaacga
     1561 cgcctgactt gcacatcagg cgacgcgcac catctctcgc aatgcgccgg tctttgaaac
     1621 tgtggaatga cgatcggcct cgggtaggcc gctctgagac tcaagtagcc ggcgaccgcg
     1681 tcgctggcgt tagcctctac aaaaatggtc ggagtgagag gattcgaacc tccgacccct
     1741 gcctcccgaa ggcagtgctc taccagactg agccacactc cgacacgaag cgcggtttat
     1801 aacggcgcgg tttccctcgc aagcctcata tcgccgagga aaagggcgtc aggggaccgc
     1861 aaaccgtccc gcgcttaagt acggtgaagt ccagaagcgc aacgcgaaaa gtgctgttgc
     1921 atccccggcg accttggcgt atctgaccgc ctcccgaggg gtcagcccct caggacgccg
     1981 gacctgctgg ggaatggtgt aatggtaaca ctccggtttt tggtaccgtc attcaaggtt
     2041 cgagtccttg ttccccagcc atccacgcct gacccgtttc gtagggctgg gcgtcgaaag
     2101 tcgtctcgcc cacgggtgag gcaaggtctt cgtaattgat tcacaaaacg ggaatacttg
     2161 gcccggccag gtccctatgt gggcccagcc gcccttacga tcgcgttcga attccacgat
     2221 cagggccgtt accctgaacc attccgcgca gcgtgcattt atctgttggg tagtcgacac
     2281 aacgacgtaa aggcccctca agcacggact gaggcggcag tgatggaact accagacatt
     2341 cgggtcatga tcgtcgaaga ccaggtcatc ctcgcgatgg atctggaaat ggtgctctcc
     2401 gacgccggtt gcgacgtcgt cggctgcgcc ctggaccgcg aaggcgcgtt cggggtcggc
     2461 gagcgcgagc ggcccaacct cgccttcgtc gacgtcaacc tgctggacgg ccggactggc
     2521 ccccagatcg cacggcgtct ggtcgaggag tacggcacgg ccgtggtctt cctgaccgcc
     2581 aaccccgaac agatccccgc gggcttcgcc ggtgcgctgg gggcggtcac caagccgttc
     2641 gacgagacca ccatcagggc cgtcgtgtcg ttcgcccgcg agttcatctt ccttcacacc
     2701 gtgggcgagg ctccgggtcg gctgcagctc gccccctggc tcacgacgca gcagtccgtc
     2761 gctcaccact gaggacgtcc cgacgacggg ctgctaggcg aacggcaacc ccggagcatc
     2821 gggcgacgtc gtctcgcccg ggcccagccg gcgctgcatg aagacgacgt cgcgccagtc
     2881 gtcgagcttc cagcccacgc cctcgatcgc tcccatcggc gcaaagccac aggcggcgtg
     2941 cagtctgatc gaccccacgt tggcgctgtc gccgatgacg cccagcatct ggcgcagccc
     3001 catggcctcg cagcgggcga tcagctccag cagcaacgcc ttgccgacgc cgcgcccgtg
     3061 agcgctggcc agcacataga tcgagtcctc caccgtgtag cgataggccg cgcgcgtccg
     3121 gaacggcccg gcgtaggcat agccgaccac cacgccctcg atctcggcca ccaaccacgg
     3181 caggccgagc gtcacgatcg ccgcccgccg cgccgtcatc tccggcacgc ccggcgcggt
     3241 cagctcgaac gtcccggtcc cggtcagcac gtcttcggcg tagatggccg tgacggcctc
     3301 caggtccgcg tccgtggcgt aacggacccg caccatcgga gctgggctca cgcggcctcc
     3361 cagcccttgt tcaccgccca gacggcgaag gcgtagtcgt gggcgatctc cttgaggtag
     3421 tcgaacctcc ccgacgagct gaagtgcccc gccgtcatgt tgatcttgag caggatcggc
     3481 ttgcccgagg tcgtggcggg cctcagcttg gcgacccact tctcgggctc ccagtaggtc
     3541 acgcgcgggt cggacaggcc ccccgtcgcc agcaccgccg gatatgccat gggggccacg
     3601 ttgtcgtagg ggctgtagct catcatgtag tcgtagtcgg cggcgctctc gatcgggttg
     3661 ccccactcgg gccactcggg cggcgtcagc ggcagtgagg tgtcggacat ggtgttgatc
     3721 acgtcgacga agggcacccc gccgatgatc ccggcccaca ggtctggccg catgttggtg
     3781 accgccccca tcagcaaccc gcctgccgac cggccctcgg cgacgatgtt gccggcggtc
     3841 gtgtagtcct cagcgatcag gtgttcggcg caggcgatga agtcggtgaa ggtgttgatc
     3901 ttggtgtgcc ggcgggccgt caggaaccag ttccagccct tgtccgaccc gccccggatg
     3961 tgggcgatgg cgtagatgaa gccccggtcc acgaggctca gccggttggt cgagaagctg
     4021 gccgggatgg ggttcccata ggacccgtag ccgtacagca gcagcggcgc cgagccgtcc
     4081 accggcgtcg tcttcttcct cagcaaggtg atcggcacca tctggccgtc gggggcaggg
     4141 gcgttcagcc gctcgacgac gtagtcggcc gggtcgtggc ctgaggggac ctcctgcgtc
     4201 ttccgcaggg tccgctcgcg cgtcgccatg tcgtagtcga aggtctggtt gggggtcgac
     4261 ggcgagttgt aggcatagcg catggtcgtg gtgtcgaact ccgacgaccc gcccagcgac
     4321 agggcgtagg cgggctcgga gacggcgatc tcatgctcgt cgccggcgcg gtcgcggaca
     4381 atgacgcggc tgttggcctc ggcccgctcc tgccgcacca gatggccctt gaacaggccg
     4441 aagccctcga tgaaccgacc tggcgcatgg gccacgatgt cggtccactc agccagcgcg
     4501 gggctcgcgg tcggggcctc gacgatgcgg aagtcgaccg cgtcctcgtt ggtgcggatg
     4561 gcccagcggt cgccccagtg gtcggcgtcg tacaggcggc ccgcgtggcg cggctcgatc
     4621 accaccggcg tcgcggtcgg gtccgaggcc gggatgtagc gaacctcgga ggtctcctga
     4681 ttgccgatcg agaccagcac gaaggcgtcg tcggacgtca cgccgacgct gatgaacatg
     4741 ccgtcgtcgg cctcctcata gaccagcgtg gtctcgccac cccgggccgg gcggcggaag
     4801 atcttgtccg ggcggccgtt gtcgtcacgg ttggtccaga agatccactg gctgtcgggc
     4861 gagaaggtga agttctcggt ggcgctgtcg atgacctcgg gcagcacctc gccggtcgcc
     4921 aggtccttca cgtagatcct gaacacctcg gagccctgcg cgtcctcggc ataggcgaac
     4981 agggtgtggt cggggctgtt ggaggtcgcg ctgacctcgg agtaggcctt gccctcggcc
     5041 agcaggttgg tgtccagcag cagctccgga tcgggcgcgc ggatgaagtt gagggtcacc
     5101 gcctcgcccg cctcattgac gaaggacccc gaacgggcga cgcgcatgta gcgcggatgc
     5161 tgctcgccgg tgcggtactc gacgtagtac tcccacgagc catcgggcga ggggaccgag
     5221 ctgtcgtcct ccttgatgcg gcccttcatc tcctcgaaca tggcttcctg catcggcagg
     5281 gtcggggcca tcatggcctc gcgataggcg ttctcctcgg tcaggtgggc cttgacgtcg
     5341 gccttgatga tcgaggggtc gcgcaggacc tgttgccagg cgtcgtcctt catccactgg
     5401 tagtcgtcga cgcggacccg accgagctgt tcgatccgca cggggacctt cttcgccaca
     5461 ggagggacag ggacgggcgc tacgggggca ggggacatgg aggttccggg ctgttgggcg
     5521 agagtggagc tggccacacc ggtcagggcg acggcggcgc tcgaggcgat cagttcgcga
     5581 cggttcatgg ggtcaggctc cgagatgggg tcaagccaga ccttgtgcga gggtatcccc
     5641 atccgtcaaa cggcaaacct gtgtccgacc gcctccaagg gcgttgcccc tctcccgccg
     5701 ggagagggag ggacccgcga gcgtagcgaa gtgggagggt gaggggttgg cgcgtccgac
     5761 cggacaccgt agcccctcac cctttcgcct tgccgatcgc ttcgctcccg gaggcttaag
     5821 gcctctcccg ccgggagagg gtaggtctca cgcccccagc gcttcccgcg acatgcgttg
     5881 cagcaggtcg ccaccttggc cgaccgcagc caaccggctc gacacgtggc ccagcacgtt
     5941 ggcggcatag acctcgtaca gatcgacctt gccttgcagc cacgccggat cgccatcctt
     6001 ggcgtccagc ctggccttcg ccgccagcgc gcccttggcc agcatccagc cgcccacgac
     6061 gtcgcccagc agtttcagat aggcgtctgc agcggccagt acatccgcgc cccgatcctt
     6121 gtcggccttg gcgtccagca gccacagggt ggcgtcctcg accgcctcga tcgccgtctt
     6181 gaaccgttcg accggcttgc cggtgtagaa cttgccgagc gaggcgagcg tggtcttcat
     6241 ctcgacgatg acggcgcggg cggcggcgcc gccgtccatc gacagcttgc ggccgacgag
     6301 atccatggcc tggatgccgt tggcgccctc
//
LOCUS       DASVTM010000472         6428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_525591,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000472 DASVTM010000000
VERSION     DASVTM010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525591"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VF633_13030"
     CDS             complement(<1..336)
                     /locus_tag="VF633_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX8472026.1"
                     /translation="MIRARRARIVATLGPASRAPGMVKALAQAGVDVFRLNFSHGSHD
                     DHAAALKAVRGAEIALQRPLGVLADLQGPKLRLGRFKDVEIDIKPGHKMRFDLDPTPG
                     DETRVQMPHP"
     gene            complement(494..2185)
                     /locus_tag="VF633_13035"
     CDS             complement(494..2185)
                     /locus_tag="VF633_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8472027.1"
                     /translation="MKSTSAIVSALLMLGAAPALAQTEALRAPLPPSNVAPAKPQLIV
                     TIVIDQFSANLFNQYRSRFTGGLRTLADQGLVYTNGYQAHGVTTTCPGHSTVLTGAHP
                     THTGLPTNEWIDPATGKEVYCLAAPRNTIAGGAVSENGLVGSDQLLATNLPDWVKAQT
                     PNSLVFAVSGKDRGAINLAGHHADGAYWIAGPMLTTYVEPGQTSEARLAPIAAFNAAY
                     KAKLAADPNPTWDFSHDYCRTLRGTAIIGGETFHADVPPEHANLEDSPYLDETTLDAA
                     TYLLESQQMGRRGVVDLLGVSLSGTDKIGHAYGTQGPEMCEQMLRMDAALGVFLGKVA
                     AVPGGAIVVLTADHGGSDFPERMHDRGAAEAGRYDPTMLTRINDQLKATFKLTDAPLQ
                     NGGTGFIIADKSKKSLAEPLRSRIIAAAIPLLNAEPTITLAVPRDELLSDPMPAPNAN
                     PEELTVRERLRLSAVAGRGADILRAYAYNLTTNGRIGGTLASHGSVWDYDRRVPIIFW
                     RPGERGEERFWPVRTIDIAPTLANLIGVPTPNTVDGRCQNLGLFDVPACTMAPAP"
     gene            complement(2336..2596)
                     /locus_tag="VF633_13040"
     CDS             complement(2336..2596)
                     /locus_tag="VF633_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472028.1"
                     /translation="MTKTETKWTFETAAEFWRKWSSSDLEPVWDRFSSAERFLLDWQP
                     ATPSQATTMLDVLLQTVASRSDGRDQTALAHLQRYIGELPAE"
     gene            complement(2593..3054)
                     /locus_tag="VF633_13045"
     CDS             complement(2593..3054)
                     /locus_tag="VF633_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472029.1"
                     /translation="MNLLQYVLLGATLIILIALLWVGQRVDRIGAAAFLLVLAISPLL
                     YPLEIGNIRWAVALMSVVFLTVLIALVIASRRWWVVAAAGFQLAATATYAIALAQPEL
                     LIWTGVSLRLVLWILQMFACLFGILEAWPHRKTRNPNHRAKRPYPSAEYIR"
     gene            complement(3082..3471)
                     /locus_tag="VF633_13050"
     CDS             complement(3082..3471)
                     /locus_tag="VF633_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472030.1"
                     /translation="MSNISRLPTLEGAKPRSEHQTKRSLGARAARELRSAEHQLDHAL
                     ASHLALAQTLVLGRIEIGVAANAGQQALLDLTRAIGFAVEARGATIRAHEGLRQTAEE
                     FSVPWRLEGPAEPKGETGIITMPTAAA"
     gene            complement(3525..3983)
                     /locus_tag="VF633_13055"
     CDS             complement(3525..3983)
                     /locus_tag="VF633_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8472031.1"
                     /translation="MSQKPLTAREKQCLELAAQRLSDQEIADRLGISPRTVAAHFQSI
                     FGKLGVRDRRRAAERVGIQYLGIAMPMSADAVVSATAGAEAVDLVPTGSEPGPAIKPW
                     SIPPAPERLVFRLVLVLMFAIVGTVLTVGGAAVLQLSLELMQASAPAGVR"
     gene            complement(4575..4784)
                     /locus_tag="VF633_13060"
     CDS             complement(4575..4784)
                     /locus_tag="VF633_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472032.1"
                     /translation="MSKSRGGTVVINMQTQMDRNPTSFTGRLIGDLFLFTLLAVVKML
                     ICIRGVVRGAREIGLLPQKDASEPC"
     gene            complement(4823..>6428)
                     /locus_tag="VF633_13065"
     CDS             complement(4823..>6428)
                     /locus_tag="VF633_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8472033.1"
                     /translation="YMGEDQSLERLKSEADAIGYPVLIKAVAGGGGKGMRRVDASEAF
                     LDALGSCRREAASSFGDDRVLIEKYILSPRHIEVQVFGDSHGEVVHLYERDCSLQRRH
                     QKVIEEAPAPGMDAATRAAITDAAIKAAKAVNYVGAGTIEFIADGTDGLRADRIWFME
                     MNTRLQVEHPVTESITGVDLVEWQFRAAAGEAVPLKQADISMNGWAMEARLYAEDPAN
                     GFLPSIGKLEHFRLPDDIRVDTGVFEGGEVSQFYDPMIAKLIVHADTREAATSKLADA
                     CGEVEVWPVKTNAGFLKRCLDHPKFVAGDVDTGFIAAEQEALIGPLEREVAVLGAAVL
                     IDGLARDADMELGIEVMGPWSPTPRSLFGFRLNASPGARLRGFADGVQADLTVKSRPD
                     WSWEVDSGEAASQVRVRWSSKHGRRGVYVDDDRVADFRDFGDEVVVFRHGVASSVSLR
                     PPREAVSGSASDGSLRAPMPGKIVAMPAKAGDTVIKGQPIVVLEAMKMEHALTAPFNG
                     VVGEIGVAVGDQVAADTVLAVVTAEA"
BASE COUNT         1164 a         2104 c         2025 g         1135 t
ORIGIN      
        1 cgggtgcggc atctgcaccc gcgtctcgtc gccgggcgtg ggatccaggt cgaaccgcat
       61 cttgtgaccc ggtttgatgt cgatttcgac gtccttgaag cgcccgagtc gcagctttgg
      121 accttggaga tcagccagca cgccaagcgg gcgttggagc gcgatctcgg cgccgcgaac
      181 cgccttgagc gccgctgcgt ggtcgtcgtg cgagccgtgg ctgaagttga ggcggaagac
      241 gtcgaccccg gcctgggcca gggccttcac catgccagga gcccggcttg cggggccgag
      301 agtggcgacg atgcgcgcgc gtcgtgctct gatcatttga ctatctcgtt gtccggtcgc
      361 gtttcctcag ccccttgcag agacctgaaa gccttttcgc aagctaacac gcactgcgta
      421 aggccggcgt cagacgggaa acacgaagtt acgtcctgta acgatctaca ggtgtccgga
      481 aggaccttgg cagtcaggga gcgggcgcca ttgtacaggc cggaacatcg aacaggccga
      541 ggttctgaca gcgtccgtcc actgtgttcg gggtcgggac gccgataaga ttggccagcg
      601 tcggcgcaat gtcgatcgtc cgcaccggcc agaaccgctc ttcgccgcgc tcgcccggac
      661 gccagaagat gatcggcaca cgacggtcgt aatcccacac cgacccgtgg ctggcgagag
      721 tgccgccaat ccgcccgttg gtggtcaggt tataggcgta ggcgcggagg atatctgcgc
      781 cccgaccagc aacggcgctc aaccgtaagc gctcccgcac cgtcaactct tccgggttgg
      841 cgttcggcgc gggcatcgga tcgctcagca gttcgtcgcg cggcacagcg agggtgatcg
      901 tcggctcggc gttcagcagc gggatggcag cggcgatgat ccggctgcgc agcggctcgg
      961 ccagcgattt cttcgatttg tcggcgatga tgaagccggt gccgccattc tgaaggggtg
     1021 catcggtcag cttgaaagtc gccttcagct gatcgttgat ccgcgtcagc atcgtcgggt
     1081 cataccggcc ggcctcggcc gctccccgat catgcatacg ttcggggaag tccgagccgc
     1141 catggtcggc ggtcaggacg acgatcgccc cgcccggaac agcggcgacc ttacccagaa
     1201 agacgccgag agccgcatcc atacgcagca tctgctcgca catctcaggg ccctgagtac
     1261 cgtaggcgtg accaatcttg tcggtgcccg aaaggctgac gcccagcaga tcgacgaccc
     1321 cgcgccgccc catctgctgg ctttccagca gataggtggc cgcatcgagc gtggtttcgt
     1381 ccaggtaggg cgagtcctcg agattggcgt gttcgggcgg aacgtccgca tggaaggtct
     1441 caccgccgat gatcgccgtc ccgcgcaggg tccggcaata gtcgtggctg aagtcccagg
     1501 ttggattcgg gtcagccgcc agcttcgcct tgtaggcggc gttaaacgcg gcgatggggg
     1561 caagccgagc ttcggaggtc tgaccgggtt cgacataagt cgtcagcatc ggcccggcga
     1621 tccagtaggc gccgtccgca tggtgaccag cgagattgat cgcgccgcga tccttgcccg
     1681 acaccgcgaa gacgaggctg ttcggggtct gcgccttgac ccagtccggc aggttggtgg
     1741 ccagcaattg gtccgatccg acaagaccgt tttcgctcac agctccgccg gcgatggtgt
     1801 tgcgaggggc tgccaggcaa tagacttcct tgcctgtcgc cggatcgatc cattcgttgg
     1861 tggggaggcc ggtatgggtc ggatgggcgc cggtcaggac cgtcgagtgc ccggggcagg
     1921 ttgtggtcac accatgggct tgatacccgt tggtatagac gagcccttgg tcggccaagg
     1981 tcctgagccc acccgtgaaa cggctccggt actggttgaa aagattggcg ctgaactggt
     2041 cgatcacgat cgtgacgatc aactgcggct tggccggcgc aacattggac ggcggcagcg
     2101 gggctctcaa tgcttccgtc tgggccagag ccggagcggc tcccagcatg agaagagccg
     2161 acacgatcgc tgaggtggac ttcactggga cgctccgatg acctgacggc ccatagcgct
     2221 ggcgcatggc ggatgcgtga cagtggggcg ggagccggtc gggcctcgta gcggttccgc
     2281 ccattttgtc cagagtctcg cgcagcagag gcgggcggcc agcttacgcc tgccgtcact
     2341 ctgccggcaa ctcaccgatg tagcgctgca gatgagccaa cgcggtctgg tcgcgcccat
     2401 cgctgcggga tgcgacggtc tgaagcagga cgtcgagcat cgtggtagcc tgactcggcg
     2461 tcgccggctg ccagtccagc aggaaccgtt cggcggaaga gaagcgatcc cagaccggct
     2521 ccaggtcgct ggaagaccat ttgcgccaga actcggcagc ggtttcgaac gtccatttgg
     2581 tttctgtttt agtcatcgga tgtactcggc agagggatac gggcgtttgg ccctgtgatt
     2641 gggattacgt gtcttgcggt gaggccaggc ctcaaggatc ccgaacagac aggcgaacat
     2701 ctgcaggatc cagagcacca gccggagcga tacgccggtc cagatcagca actctggctg
     2761 agccagtgcg atcgcgtaag tcgcggtcgc tgccaactga aagcccgccg ccgcgacgac
     2821 ccaccagcgc cgcgaggcga tcacgagagc gataaggacg gtcagaaaca cgacggacat
     2881 cagggctacg gcccagcgaa tgtttccgat ctccaacgga tacagcagcg gcgaaatcgc
     2941 aaggacgagc agaaacgccg ccgccccgat ccgatccacc cgctgaccga cccacagcaa
     3001 agcgatcagg atgatgagcg tcgcgcccag gaggacgtac tgaagaaggt tcatcgccgt
     3061 tcgccggtta cggggtccgg atcaagctgc agccgtgggc atggtgatga tgccggtttc
     3121 gccttttggc tccgctgggc cttcaagacg ccatggaacc gagaattcct ctgcagtctg
     3181 acgcaggcct tcgtgagctc gaatggtggc gccccgcgcc tcgacggcga acccgatcgc
     3241 gcgggtcagg tcgagcagcg cctgctgacc tgcattggcg gcgacgccga tctcgatccg
     3301 ccctagaacc agtgtttggg cgagggcgag atgcgacgcg agggcgtgat cgagttgatg
     3361 ctctgcactg cgcagttccc gagcggccct ggcgcccagc gaccgcttcg tctggtgctc
     3421 ggaccggggc ttggcccctt cgagggttgg cagtcggctg atgttggaca tggtggatat
     3481 cctgccgcac acatgcggaa ttgagtgtcg tcgtgacctt ggcctcagcg gactccggcg
     3541 ggggccgagg cctgcatcag ttccagactg agttgcagaa ctgcggctcc gccgacggtc
     3601 aacaccgtcc ccacgatcgc gaacatcaga accagcacca ggcgaaagac caaccgttcc
     3661 ggagcgggcg ggatggacca cggcttgatt gcaggaccgg gctcggaacc cgttggaacg
     3721 aggtcaaccg cctcggcccc tgcagttgcg ctgaccaccg cgtcggcgga catcggcatt
     3781 gcgataccta ggtattgaat accgacccgc tcggcggcgc gccgcctgtc gcgaacgccc
     3841 agcttcccga aaatgctctg aaaatgcgcg gccaccgtcc gcgggctgat gcccagccgg
     3901 tcggcaattt cctggtcact caaccgctgg gcggcgagtt ccagacattg cttttcacgc
     3961 gccgttagcg gcttctgcga catgaatatt cccccgcagt taacctaggc gcgcgacccg
     4021 ggcgccgtcc agcatcctcc catcccatgg tgtgggaaac catgacagcg gtttggaaga
     4081 tagccaggac gacgttcccc ggccgggccg cgacgcgccg atgaacctgt cgaatggtta
     4141 cacagggttg ggagagtatg gtgcgggcgg ccggactcga accggcatgg gcatacgccc
     4201 ggcggatttt cataccactt cgactttcgc cgccgctccg gtacgcccgg atcgttcgtg
     4261 gtctggacta tcccttcgcc atgacccgtg gcccgtaaaa gccgcagatt ttagacgccg
     4321 cccgtctagt ctctacacct tcccaaagga ctacgcggtc cgacgggctt ggctcgggat
     4381 cgccatctta cagggttccc cgaatttgag cggttctacg tccgggattt cccccggcgc
     4441 actcaatcct cagcttaagt ccgcagcgtc taccattccg ccacgcccgc aggccgagtg
     4501 cagctatatc cagcctcgac gtccgcgcca aggcgttgcg ggtcatccga ggcgacgaag
     4561 cgtgctgttc aggcttaaca gggttcggac gcgtcctttt gcggtagaag accgatctcc
     4621 cgcgcccccc tcacgacgcc acggatgcag atcaacattt tgaccacagc gagcagggtg
     4681 aagaggaaca ggtcgccgat caatcggccc gtgaacgacg ttggattgcg atccatctgg
     4741 gtctgcatat tgatcaccac tgtcccgcca cgagacttcg acaatgaggc gagcaaatcc
     4801 aatgccacac gaaccgacgg gatcaggctt cggcggtgac cacggccagg accgtatcgg
     4861 ccgcgacctg atcgcccacc gcgacaccga tctcgccgac cacgccattg aagggcgccg
     4921 tcagcgcatg ctccatcttc atcgcctcca gcaccacgat cggctgtccc ttaatcacgg
     4981 tgtcgccagc cttcgcaggc atcgccacga tcttgcccgg cattggagcg cgcagcgagc
     5041 cgtcggaggc ggagcccgac acagcctcgc ggggtggccg aagcgaaaca gacgaggcca
     5101 cgccgtgccg gaacaccacg acctcgtctc cgaagtcgcg gaagtccgcc actcggtcgt
     5161 catccacata gacgccccgt ctgccgtgct tgcttgacca gcgaacccgg acctgcgacg
     5221 cggcttcgcc ggaatcgacc tcccacgacc agtccggccg ggacttgacg gtcaggtccg
     5281 cctgcacgcc atcggcaaag ccgcgaaggc gcgcgcccgg cgacgcattc aaccggaacc
     5341 cgaaaagcga ccgtggtgtg ggcgaccaag gtcccatgac ctcgatgcct aactccatat
     5401 cggcgtcccg tgcgagcccg tcgataagga cagcggcgcc tagaacggcg acctccctct
     5461 ccagagggcc gatcagcgcc tcttgctccg ccgcgatgaa acccgtgtcc acgtcgccgg
     5521 cgacaaattt cggatgatcg agacagcgtt tcaggaaacc cgcattagtc ttcaccggcc
     5581 agacctcgac ttcgccgcag gcgtccgcca gcttcgacgt cgccgcctca cgcgtgtcgg
     5641 catggacgat cagcttcgcg atcatcgggt cgtaaaactg gctgacctcg ccgccctcga
     5701 acacgccagt gtcgacgcga atgtcgtctg gcagccggaa atgctccaac ttaccgatac
     5761 tgggcaggaa gccgttcgcg ggatcttcgg catagagccg cgcctccatg gcccaaccgt
     5821 tcatcgagat gtccgcctgc ttgagcggca ccgcctcccc cgccgccgcg cggaactgcc
     5881 attcgaccag atcgacgccc gtgatcgact ccgtcaccgg atgctcgacc tgaagccggg
     5941 tgttcatctc catgaaccag atgcggtccg cgcgcaggcc atcggtgccg tcggcgatga
     6001 actcgatcgt tcctgccccg acgtagttca cggccttggc ggccttgatc gctgcgtcgg
     6061 taatcgccgc ccgcgtcgcc gcatccatgc cgggcgccgg ggcttcctcg atgaccttct
     6121 ggtgccggcg ttgcagcgag cagtcgcgct cgtacaggtg gaccacctcg ccgtggctgt
     6181 cgccgaacac ctgaacctcg atgtggcgcg ggctcaggat gtatttctcg atcagcaccc
     6241 ggtcgtcgcc gaacgaggac gccgcctcac gccgacacga tcccagcgca tcaaggaagg
     6301 cctcagacgc atcaacccgc cgcatcccct tgccgccgcc gccggcgacc gccttgatca
     6361 gcacgggata tccgatggcg tcagcttctg acttcaaccg ctcaagcgac tggtcctcgc
     6421 ccatatag
//
LOCUS       DASVTM010000473         4512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_312094,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000473 DASVTM010000000
VERSION     DASVTM010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312094"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VF633_13070"
     CDS             <1..920
                     /locus_tag="VF633_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX8472034.1"
                     /translation="NAAIRGFIINKFRGDPKLFDDGRTEIVRRTGWPDMGLVPWLASA
                     RSLPAEDAVVLSEAVEAGSRRVKIAIPILRHIANFDDFDPLQAEPSVELVYVPPGRPL
                     PGDADLIILPGSKATLSDLAFVRTQGWDIDLKAHVRRGGRVLGICGGFQMLGRTVADP
                     DGIEGRAHSAEGLGLLDIDTVIGGDKVLEPCSGRTVLGDGAYEGFEMHMGRTTGEGLT
                     RPLLRREDGSWEGAVSADGRVSGCYVHRLFDSGSARAALLAELGVASQAIDQRIAVDA
                     ALDEIAAALEIHLDIAALARLAGLKDFEA"
     gene            917..1924
                     /gene="cobT"
                     /locus_tag="VF633_13075"
     CDS             917..1924
                     /gene="cobT"
                     /locus_tag="VF633_13075"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX8472035.1"
                     /translation="MTGITEAMIRTHLDAQARPPGSLGRIEDLAVRLALIQQTLTPCA
                     DKATLLVFAGDHGLTEDGVSRYPAFVTQAMVATLLAGKATANAFARAVGAEVVIIDAG
                     VNADLPSHPSLIDAKVRKGTRNAAREPAMTLEEAKLALTRGAEQATTAIDRGADLLVL
                     GEMGIGNSASAALLMHRLTPAPLNDCVGVGTGHDAAGLAHKLAVLIRAAERSDATAPL
                     EVLRQFGGFEIAMMAGAIHGAADRNTPVLVDGFICTAAALAAIRLRPKVREVCIFAHR
                     SAEAGHDRMLKALDAEPLLDLGLRLGEGTGGLLALPLLRAAAALLSDVASLDDVMAGR
                     I"
     gene            1926..2690
                     /gene="cobS"
                     /locus_tag="VF633_13080"
     CDS             1926..2690
                     /gene="cobS"
                     /locus_tag="VF633_13080"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX8472036.1"
                     /translation="MPRQFALFLTAVQFLTRVPVPTLRDFQPDWTARSARYFPLVGTL
                     VGVASGLVLIAASHVWTGVLPALLATAAGILITGAFHEDGLADAADGLGGGQTPGRRL
                     EIMKDSRVGTYGVLALVLGVALKVAALAQTPIPLVLASLVAAHACGRGAAVLAMRLLP
                     YAGDRASTKVKPVADGVTTGETLVALTFAALAMIPVLLLAPVATLGALVVAGVCAVVM
                     ALLAKRLIGGWVGDTLGATEQAFEIGFLVVFAGLIA"
     gene            2687..3187
                     /locus_tag="VF633_13085"
     CDS             2687..3187
                     /locus_tag="VF633_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472037.1"
                     /translation="MSAIIVAPLHEVDEAIRIWAPSHVIGLASPGAEIAGPVEGVERL
                     QLTFHDIAEPQPGLQPVTENDVERLLAFGQSWSGAAPLMIQCWAGVSRSTAAAYAIAC
                     MVSSSDEDQIARALRLAAPFATPNRRLVALADAALRRDGRLIAAISGIGRGAEVSMGS
                     TFRLQV"
     gene            complement(3194..>4512)
                     /locus_tag="VF633_13090"
     CDS             complement(3194..>4512)
                     /locus_tag="VF633_13090"
                     /inference="COORDINATES: protein motif:HMM:NF045160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472038.1"
                     /translation="RERLAELLLQATVGDTNLKRLLKLELAAEVGAPDLALEIDKRLA
                     ALEASKTRVSWRKRPELVADLQVHRRMIVDRLAALDPVMGLDRLVAWFDLYPGLTERV
                     KDPKGELSNVFFDAAPDLATVATTVGQDAVAALAEALETRLSEWAGWIGRAAPTMSEA
                     VAASLLHRLTAGRPKPVGKRALVVRKLADRAGDVASWASSFTDEDRQKPDVGAEIARR
                     LALAGRPVEARAALEASKPRAPAPARWTTRSRPAPEPEPSDAWEAAEIAVLDAEGRPD
                     EAQTARWIAFERTLSQAPLRAHIAKLADFDDVEAIDRALAHAAGWYDATRGLAFLMNW
                     PALLEAATMILARGDELRATAEDTALWAGRLENRYPNAALALIRSRAKALSRQGLGRT
                     DEVRTLSAEAAAMTELPGALNGIPSHAEFIDELEALSPTARARQWR"
BASE COUNT          647 a         1498 c         1589 g          778 t
ORIGIN      
        1 acaatgcggc catcaggggc ttcatcatca acaagttccg gggtgatccc aagctgttcg
       61 atgacggccg gaccgagatc gtgaggcgta ccggatggcc cgacatgggc ttggttccgt
      121 ggctggcgtc tgcacggagt cttccggccg aggatgcggt ggtgctcagc gaagcggtcg
      181 aggccgggtc gcgccgggtg aagatcgcca tcccgatcct gcgccacatc gccaatttcg
      241 acgacttcga tccgcttcag gccgagccct cggtcgagct ggtctatgtg ccgccgggcc
      301 ggcccctgcc gggcgacgcc gacctgatca tcctgccggg ctccaaggcg accctgtcgg
      361 atctggcgtt cgtgcggacg cagggctggg acatcgacct caaggcccac gtccggcgcg
      421 gtggtcgggt tctgggcatc tgcggcgggt tccagatgct gggccgcacc gtcgccgatc
      481 cggacgggat cgagggccgc gcgcattcgg cggagggtct gggcctgctc gacatcgaca
      541 cggtgatcgg cggcgacaag gtgctggaac cctgtagcgg tcggaccgtc ctcggcgacg
      601 gggcctacga ggggttcgag atgcatatgg ggcgcacgac cggcgaaggc ctgacccggc
      661 cgctgctgcg tcgcgaggac ggcagctggg agggcgcggt ttccgccgat ggtcgggtgt
      721 ccggctgcta tgtccaccgc ctgttcgaca gcggctcggc gcgggcggcg ttgctggcgg
      781 aactgggtgt cgcctcgcag gcgatagatc aacggattgc cgttgatgca gcgctggacg
      841 agatcgccgc tgctctggaa atccatctcg acatcgcggc tctggcgcgc ctcgccgggc
      901 tgaaggactt cgaagcatga ccggaatcac cgaggcgatg atccgcaccc acctcgacgc
      961 ccaggccagg ccgccgggct cgctcggtcg gatcgaggat ctggccgtcc ggctggcgct
     1021 gatccagcag accctgactc cgtgcgcgga caaggcgacg ctgctggtgt tcgcaggcga
     1081 ccatggcctg acggaggacg gcgtgtcccg ctatccggcg ttcgtcaccc aggcgatggt
     1141 cgcgaccctg ctggcgggca aggcgaccgc caacgccttc gcccgtgcag tgggggcaga
     1201 ggtggtcatc atcgacgccg gcgtgaacgc ggacctgccc tcccatccct ctctgatcga
     1261 cgccaaggtg cgcaagggga cgcggaacgc cgcgcgcgaa ccggcgatga cgctggagga
     1321 ggcgaaactg gccctgacgc gcggcgccga acaggcgacc accgcgatcg atcgcggagc
     1381 cgacctgctg gtcctcggcg agatgggcat aggcaacagc gcctcggctg ccctgctgat
     1441 gcatcggctg acgcccgcgc cgttgaacga ctgcgtcggc gtcggcacgg gtcacgacgc
     1501 agcgggtctg gcccacaagc tggccgtcct catccgcgcc gccgaacgct cggacgcgac
     1561 cgctccgctg gaggtgctgc gtcagttcgg cggcttcgag atcgccatga tggccggtgc
     1621 gatccacggg gcggccgacc ggaacacgcc ggtgctcgtg gacggtttca tctgcaccgc
     1681 cgccgctctc gccgcgatcc ggctacggcc aaaggtgcgc gaggtctgca tttttgccca
     1741 ccgatcggcc gaggccgggc acgaccggat gctgaaggcg ctcgacgcgg aacccctgct
     1801 cgatctcggc ctgcgactgg gcgagggcac tggggggctt ttggctctgc ccctgctgcg
     1861 tgcggcggcg gccctgttgt ctgacgtcgc cagtctcgat gatgtcatgg cagggcggat
     1921 ctgagatgcc gcgccagttc gccctgttcc tgacggcggt gcagttcctg acgcgcgtgc
     1981 ccgtgccgac gctaagggat ttccaacctg actggacggc gcgatccgct cgctattttc
     2041 cgttggtagg caccttggtc ggggtggcgt cggggctggt tctgatcgcc gcctcacacg
     2101 tctggactgg ggtgttgccc gccctgctgg cgaccgctgc cggaatactg atcaccggag
     2161 ccttccacga ggacggcctc gccgatgccg ccgatgggct tggcggaggc cagacgcccg
     2221 gacgccgtct tgagatcatg aaagacagcc gcgtcggcac ctacggagtt ctagcccttg
     2281 ttctgggcgt cgctttgaag gtcgcagcgc tggcgcagac ccccataccg ctagtgctcg
     2341 cgtcgctggt cgccgcgcac gcgtgcggac gaggggcggc ggttctggcc atgcgactgc
     2401 tgccctacgc gggcgaccgg gcctcgacca aggtcaagcc ggtcgctgac ggcgtgacga
     2461 cgggggaaac gctggttgcc ctgacgttcg ccgcgctggc gatgatcccg gtgctcctac
     2521 ttgcgccggt tgcgacgttg ggcgcgctgg ttgttgcagg cgtgtgcgcg gtggtcatgg
     2581 cgttgctggc gaagcggctg atcggcggct gggttggcga tacgctcggt gcgacggagc
     2641 aggcgttcga gatcggcttc ctcgtcgtct tcgcaggctt gatcgcgtga gtgcgatcat
     2701 cgtcgcgccg ctgcatgagg tcgatgaagc catccggatc tgggcgccgt cgcacgtgat
     2761 cggtctggcg tctccgggcg cagaaattgc aggtccggtc gaaggcgtcg aacggctgca
     2821 actcaccttc cacgacatcg cagagccgca gccgggcctg cagcctgtta cggagaacga
     2881 tgtcgagcga ttgctggcgt tcggccaaag ctggtcaggc gccgcaccac tcatgatcca
     2941 gtgctgggcg ggggtcagcc ggtctacggc ggcggcctat gcgatcgcct gcatggtcag
     3001 ttcgtccgac gaggaccaaa ttgcgcgagc gctcagactg gccgcaccgt ttgcgacgcc
     3061 gaaccgcagg ctggtcgccc tcgcggacgc cgcactccga cgagatggcc gtctcatcgc
     3121 agccatctct ggcatcgggc gtggcgccga ggtctcgatg gggtcgacct tcaggctgca
     3181 ggtctgatcc ggctcatcgc cactgccgag ccctggctgt cggcgacaga gcttccagtt
     3241 cgtcgataaa ttctgcgtgg ctcgggatac cgttcaaggc gccgggcagt tccgtcatcg
     3301 cggcggcctc cgccgacagg gttcgcacct cgtccgtgcg tccgagccct tggcggctga
     3361 gcgccttggc tcgagaccgg atgagcgcca gcgcggcgtt ggggtagcgg ttctccagac
     3421 ggccggccca caaggccgtg tcctcggctg tggcgcgcaa ctcgtcgccg cgcgcgagga
     3481 tcatggtcgc ggcttccagc aacgcgggcc agttcatcag gaaggccaga cctcgcgtgg
     3541 cgtcgtacca gcctgcggcg tgggccaatg cacggtcgat cgcctcaacg tcgtcgaagt
     3601 cggcgagttt ggcgatgtgg gcccttagcg gcgcctgcga cagcgtccgc tcgaacgcga
     3661 tccagcgtgc cgtttgcgcc tcgtccggcc gcccttcggc gtcgaggacg gcgatctcgg
     3721 cggcttccca ggcgtctgac ggctcgggct ccggcgcggg cctgctgcgt gtcgtccagc
     3781 gggccggcgc cggggcgcgc ggcttcgaag cttcgagcgc ggcgcgggct tcaacgggac
     3841 gccctgcaag agccagtcgc cgggcgatct cggcgccgac gtctggcttt tgtcggtcct
     3901 catcagtgaa ggatgaagcc cagctcgcca cgtcacccgc acggtcggcg agcttacgca
     3961 ccacgagggc gcgttttccc accggcttcg gtcgtcccgc ggtgaggcgg tggagcaggc
     4021 tcgcagccac cgcctcgctc attgttggcg cggcgcgacc gatccacccg gcccattctg
     4081 acaggcgggt ctcaagcgct tcggccagcg cggcgacggc gtcctgtccg acggtggtag
     4141 cgacggtcgc cagatcaggc gcggcgtcga aaaagacgtt tgaaagttcg ccctttggat
     4201 ccttcacccg ttcggtcagg cctgggtaga ggtcgaacca cgccaccagc cgatccagcc
     4261 ccatcaccgg gtccagcgcc gccagccggt ccacgatcat gcgacggtga acctgaaggt
     4321 cggcgaccag ttcgggccgt ttgcgccacg agacccgcgt cttgctggcc tccagcgccg
     4381 ccaaacgctt gtcgatctcc aatgccagat cgggcgcgcc gacctcagcc gccaattcga
     4441 gcttgagcag acgcttcaga ttggtgtcgc cgaccgttgc ctgaagcagc agctcggcaa
     4501 ggcgttcacg ac
//
LOCUS       DASVTM010000474         5730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_631738,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000474 DASVTM010000000
VERSION     DASVTM010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631738"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VF633_13095"
     CDS             complement(<1..678)
                     /locus_tag="VF633_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8472039.1"
                     /translation="MARTHALEDYRNFGIMAHIDAGKTTTTERILYYTGKNHKIGEVH
                     DGAATMDWMDQEQERGITITSAATTAFWQGKRLNIIDTPGHVDFTIEVERSLRVLDGA
                     VAVLDGNAGVEPQTETVWRQADKYNVPRIVFVNKMDKIGADFDASVQSIRDRLGAKAV
                     PIQFPIGSENTLSGLVDIVSMNAVVWDNDALGANFTVGPIPADLVDKANEARQYLIDN
                     AVELDDEA"
     gene            complement(827..1300)
                     /gene="rpsG"
                     /locus_tag="VF633_13100"
     CDS             complement(827..1300)
                     /gene="rpsG"
                     /locus_tag="VF633_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX8472040.1"
                     /translation="MSRRHRAQKREVLPDPKYKDLTVTKFMNYVMYEGKKAVAENIVY
                     GAFEILADKKKDFEAVQTFHTALENVQPSVEVRSRRVGGATYQVPVEVRPDRRRALAI
                     RWLVNAARKRGENTMTEKLAAELLDASNNRGTAVKKREDTHKMAEANRAFSHYRW"
     gene            complement(1370..1744)
                     /gene="rpsL"
                     /locus_tag="VF633_13105"
     CDS             complement(1370..1744)
                     /gene="rpsL"
                     /locus_tag="VF633_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX8472041.1"
                     /translation="MPTINQLIRKPRKPKPVRNKVPALEGSPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLAKSGYEAVCYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHILRGV
                     LDTQGVKDRKQRRSHYGTKRPK"
     gene            2530..2850
                     /locus_tag="VF633_13110"
     CDS             2530..2850
                     /locus_tag="VF633_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472042.1"
                     /translation="MIREGRSAAVGRALRNGALGCRSGVSDQEQQARCDQSFGMAAAR
                     AAPILGTDNPARDARFAAEGQAELDAYENLRAPLKPPQLCAGADMMGRCPAIITIPLF
                     RKKF"
     gene            2847..3710
                     /locus_tag="VF633_13115"
     CDS             2847..3710
                     /locus_tag="VF633_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472043.1"
                     /translation="MSADVLPSSDSLKRKSPSRRDRKSAAILAGSVALHGVVLGLIGL
                     GIFDSDLALPQPPSDAPIFIEMEPRPLLDGETARVPTTAPARATDAPALTSPLSPSIA
                     PRKLDEQDDAPTAPAPRAAAGGAAGGAAAPAGAANPWSYQPESRGAAVARTMRTGAGG
                     CRIMDGHLSAAEQALCDERFNEGAAAAAARHPLGNRTLTPSEQRRQAEFAAEGRRALE
                     RYEARRAPLRGGTGNVTTGDCPGSNFGTGCAGANLEPSMREGATSTIRQDSNLLPSDQ
                     HKPVPGHEPQN"
     gene            complement(4160..>5730)
                     /locus_tag="VF633_13120"
     CDS             complement(4160..>5730)
                     /locus_tag="VF633_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX8472044.1"
                     /translation="VKVRSALTCEAEAGICGMCYGRDLARGTNVNIGEAVGVIAAQSI
                     GEPGTQLTMRTFHIGGTAQVAETSFYEATNAGVVKIVGPTVTGAHGDLVSMSRNVVVT
                     VMVDGKDRESYKIPYGGRLRVKEGSEVKKNQRLAEWDPYTTPIITEVGGVVRFEDLTE
                     GLSFKEETDEATGIAQRVVIDWRASPRGSDLRPAMGVTVGDAYAKLASGSDARYLLPV
                     GAILSVANGDTVKPGEILARIPTEGAKTRDITGGLPRVAELFEARRPKDCAVIAEMDG
                     RVEFGRDYKNKRRIKITPELNADGEQGEPVEFLIPKGKHISVHDGDLIQKGDYIIDGN
                     PDPHDLLRIQGVEALAEYLVNEVQEVYRLQGVPINDKHIEVIVRQMLQKVEILDSGET
                     TLIRGDTVEVAEAVFENEKVEKRGGRIATTQPVLLGITKASLQTRSFISAASFQETTR
                     VLTDASVHGKKDMLEGLKENVIVGRLIPAGTGAYLRSLQKVANERDALLTSTRESAVE
                     PLPADLQLELAESE"
BASE COUNT          978 a         1811 c         1843 g         1098 t
ORIGIN      
        1 ggcttcgtcg tcgagctcga cggcgttgtc gattaggtac tggcgagctt cattggcctt
       61 gtcgaccaga tcggcgggga tcgggccgac agtgaagttg gcgcccaagg cgtcgttgtc
      121 ccagaccacg gcgttcatcg acacgatgtc gacgaggccg gacagggtgt tttccgaacc
      181 gatcgggaac tggatcggca cagccttggc gcccagacgg tcgcggatcg actgcaccga
      241 ggcgtcgaag tcagcgccga tcttgtccat cttgttgacg aacacgatcc gcggaacgtt
      301 gtacttgtcg gcctggcgcc agacggtttc ggtctgcggc tcgacgccgg cgttaccgtc
      361 cagcactgca acagcgccgt cgagcacgcg cagcgagcgt tcgacttcaa tggtgaagtc
      421 cacgtggccg ggggtgtcga tgatgttcag gcgcttgccc tgccagaagg cggtcgtcgc
      481 ggccgacgta atggtgatgc cgcgctcttg ttcctggtcc atccagtcca tggtcgcagc
      541 gccgtcgtgg acttcgccga tcttatggtt cttgccggtg taatacagga tccgctcggt
      601 cgtcgtcgtc ttgcccgcat cgatgtgggc catgatgccg aagttgcggt agtcctcgag
      661 cgcgtgtgta cgggccatga aagcgtgcct tgaaagtttt caggacgcgg agagcgccct
      721 cgggaagatc gaacggttct ggataagcga gcgcgggcga tttaactgaa atcgcccgcg
      781 ccggatagca cgtagatagt gaccgtagac ggcgctttta agacgcctac cagcggtagt
      841 ggctgaaggc gcggttggct tcggccatct tgtgggtgtc ttcacgcttc ttgaccgcgg
      901 tgccgcggtt attcgaggcg tccagcagct cggctgccag tttttcggtc atggtgttct
      961 caccacgctt gcgcgcagcg ttcaccagcc aacggatagc cagggcgcga cgacggtccg
     1021 gacggacctc gacaggcacc tgataggtgg cgccgccgac gcgacgcgag cgaacttcga
     1081 ccgacggctg gacgttttcc agagcggtgt ggaaggtctg gaccgcttcg aagtccttct
     1141 tcttgtcggc caggatttcg aacgcaccgt agacgatgtt ttctgcgacg gcctttttgc
     1201 cctcgtacat gacgtagttc atgaacttgg tgacggtcag atccttgtat ttgggatccg
     1261 gcaggacttc gcgtttctgg gcgcggtgac gacgtgacat atggtgggcc ttctaaatcg
     1321 ttcgtgagaa gccagatgga accggctgaa tctgtgagtg cggttcggct tacttcggac
     1381 gcttggtgcc gtagtgcgaa cggcgctgct tgcggtcctt gacgccttgc gtatcgagaa
     1441 cgccgcgcag gatgtggtaa cggacgcctg gaaggtcttt cacgcggccg ccgcggatca
     1501 gaacaaccga gtgttcttga aggttgtggc cttcgccggg gatgtagcaa acggcttcgt
     1561 agccggactt tgccagacgg accttggcga ccttacgcag agccgagtta ggcttcttcg
     1621 gggtcgtcgt ataaacgcgc gtgcaaacgc cgcgacgctg gggcgaacct tccagagccg
     1681 gcaccttgtt gcgcacgggc ttgggcttgc ggggcttgcg aatgagctgg ttgatcgtag
     1741 gcatatgtaa aactcgtctc gacttctgat ggaggcgtgt gcctgtcggc cggggcgcct
     1801 catgtccttc ttttgaacgg cgccgacggc gtcgcccggg gtctttgagg ccggtctcga
     1861 taaaccaaac agccccggcg cgcctccggt aagccggaaa acacaaaggg ccggaacgcg
     1921 cgctttgggc gttccggcgg gcacagcccg tccgttcaat tgtcacgatc acggcgaagg
     1981 acgaatccct catgcctcga aagtccggcg cgtatatgcg cgaaggggtg tgaggtcaag
     2041 actcagggtc ttcgcctgtc cccagtgccg caaagtgcgc cccaatacgg ccaccctact
     2101 accttctgtt gcaccgtgac gggagggcgg atgcctggga cacacagatc tgacgggact
     2161 gcgtctgaaa agcgtcgctc cctcttcctg agaaaagacg ctgcggtcat tgcagggtct
     2221 gtcgccgccc acgccgtggt tttcacagtg ctcgggctcg gatggctgga agacgacaac
     2281 cgaccgagcc ccgtcccgcc ccatcagccg atctatttag acaacgagcc ttggccgttc
     2341 gtaagcggtt cgctgcgaaa gtccgcctca cccgtccggt cgcaggagac caaccccctg
     2401 gttgaaaccc ggccgtccac gtcattgatc cagagagcca tcgccggaga cgcaccgagg
     2461 gccgcctctg ctgaaacggc tgagcggacc ccggtcgcat cgccggcgtc cggcgcagaa
     2521 ccatgggccg tgatccgtga aggccgatcc gcagcagtgg gacgcgctct gcgcaacggc
     2581 gccctgggtt gccgttcggg cgtcagcgat caggagcagc aagcccgatg cgatcagtcc
     2641 ttcgggatgg ccgcggcccg tgccgcgccc attttgggca ccgacaatcc ggcccgggac
     2701 gcgcgcttcg ccgcagaggg tcaggccgaa ttggacgcct atgaaaacct gcgggcgccg
     2761 ctgaagccgc cgcaattgtg cgcgggcgct gacatgatgg gccgatgccc ggcgataatc
     2821 acgatccccc tgttccggaa aaagttttga gcgctgacgt cctgccatcc tccgactccc
     2881 tgaagcgcaa gagcccctcg cgtcgcgatc ggaagagcgc agcgatcctg gccgggtccg
     2941 ttgcactaca tggcgtggtt ctgggcctga tcgggctcgg gatattcgac agcgacctcg
     3001 ccctgcccca gcccccctcc gacgcaccga tcttcatcga gatggagcca cgccctctgc
     3061 tggatggcga gactgcgcgc gtgccgacga ccgcgcctgc gcgagcgact gacgcccccg
     3121 ccctgaccag tcctctgagc ccctccatcg caccgcgaaa gttggacgag caggatgatg
     3181 cgcctacggc tcctgccccg cgtgccgcag ccggtggagc cgccggcggc gccgccgcac
     3241 ctgcaggtgc ggccaatccc tggtcctatc aacccgagag ccgcggcgcc gccgtcgccc
     3301 gcacgatgcg gaccggcgcc ggcggctgcc ggatcatgga cggccacctc agcgcagccg
     3361 aacaggccct atgcgacgaa cgcttcaacg aaggcgccgc agctgccgcc gcccgtcacc
     3421 ctctcggcaa ccgcaccctt acgccgagcg aacagcgccg acaggcggag ttcgccgcag
     3481 aaggccgtcg cgcgctcgaa cgctatgaag cccgtcgcgc accgctgagg ggcgggaccg
     3541 gcaacgtcac gaccggcgac tgtccgggca gcaatttcgg cacgggctgc gccggggcca
     3601 atcttgagcc ctcgatgagg gaaggcgcaa cgagcacgat ccgtcaggac agcaatcttc
     3661 ttccgagcga ccagcacaag ccggtgcccg gccatgagcc acagaactag ggctggatct
     3721 ggagaaaacc gtcttaacgt caatatgcgg cgccggctcg cgattgaggt aaaacaggcg
     3781 cctgcctaac cctttgtccg cggcaggccg tgctgcgtac agttgatgtc gtgcgcgatg
     3841 gcgcctcgct gcatcgcata ggtgctaacc tcggcgtaaa gccaaccagc tgccagacag
     3901 gtgcaggcga tgctgaatgc caaacccgcg ctgcatcgcg tagcccttgg tctcatagcg
     3961 atcctccccg acaaccttga gtttggttct ggcctcagtg cgcgtcaatg tcggaaattg
     4021 ccctccgacg tgcgcttccg cctcgtctct cgcgctcgaa tagtgcgaca ccgacgccgt
     4081 taacgatagc gccctcaagc aaaaaagggc gggaccgtcg ccggtcccgc cctcgttcac
     4141 ttcacgatgt ccgaagcgac tactcgcttt cggccagttc cagttgcagg tccgccggaa
     4201 gcggctcgac tgcagattcg cgcgtcgagg tcagcagggc gtcgcgttcg tttgcgacct
     4261 tctgcagcga ccggaggtag gcgccggtgc cggccgggat cagtcggccg acgatgacgt
     4321 tttccttcag gccttccagc atgtccttct tgccgtggac cgaggcgtcg gtgaggacgc
     4381 gggtcgtttc ctggaaggac gccgccgaga tgaagctgcg ggtttgcagc gacgccttgg
     4441 tgatgcccag caggaccggc tgggtggtgg cgatacggcc gccacgcttt tcaaccttct
     4501 cgttttcgaa cacggcttca gccacttcga ccgtgtcgcc acggatcagg gtggtttcgc
     4561 ccgagtcgag gatctcgacc ttctgcagca tctggcgaac gatcacctcg atgtgcttgt
     4621 cgttgatcgg cacgccttgc agtcgataga cttcctgcac ttcgttgacg agatactcgg
     4681 ccagcgcctc gacgccctgg atacgcagca gatcgtgcgg atcggggttg ccgtcgatga
     4741 tgtagtcgcc cttctggatc agatcgccgt cgtggacgga gatgtgtttg cccttgggga
     4801 tcaggaactc gaccggttcg ccctgctcgc cgtctgcgtt gagctcaggc gtgatcttga
     4861 tgcggcgctt gttcttgtag tcgcgcccaa actcgacgcg accatccatc tcggcgatga
     4921 ccgcgcaatc cttcggacgg cgtgcttcga acagttcggc gacgcgcggc agaccgccgg
     4981 tgatgtcgcg ggtcttggcg ccttcggtcg ggatacgcgc caggatttcg ccgggcttga
     5041 ccgtatcgcc attcgccacc gagaggatgg cgccgaccgg cagcaggtaa cgggcatcag
     5101 atcccgaagc cagcttggcg taggcgtcgc cgacggtgac gcccatggcc ggacgcaggt
     5161 ccgaaccacg aggtgacgca cgccagtcga tgacaacgcg ctgtgcgatg ccggtcgctt
     5221 cgtcggtctc ttccttgaag gacaggcctt cggtgaggtc ttcgaagcgg acgacgccac
     5281 cgacttccgt gatgatcggg gtggtgtagg ggtcccactc cgccagacgt tggttcttct
     5341 tgacctcgga gccctccttg acgcgcagac ggccgccgta agggatcttg tagctttcgc
     5401 gatccttgcc atcgaccatg acggtgacga caacgttgcg gctcatcgaa accagatcgc
     5461 cgtgggcgcc ggtgacggtc ggtccaacga tcttgacgac gccggcgttg gtcgcctcgt
     5521 agaacgaggt ttccgccacc tgggccgtac cgccgatgtg gaaggtccgc atcgtcagct
     5581 gggtgccggg ctcgccgata gactgcgccg cgatgacgcc gaccgcttcg ccgatgttga
     5641 cgttggtgcc gcgcgccagg tcgcgaccat agcacatgcc gcagataccg gcttcggctt
     5701 cgcaggtcag ggccgaacgg accttgaccg
//
LOCUS       DASVTM010000475         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_258197,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000475 DASVTM010000000
VERSION     DASVTM010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258197"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VF633_13125"
     CDS             complement(<1..517)
                     /locus_tag="VF633_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX8472045.1"
                     /translation="MMKPRQDLVPNTADYEITPLVKPTGFREYDARWILEKEINLLGI
                     QALGLALATYVHEIGVQPRIVVGHDFRSYSLSVKQALIIGLLEGGMEVLDIGLALSPM
                     AYYGQFALNAPCVAMVTASHNENGWTGVKMGAQPPLTFGPDEMGRLKTRVLEGQGVAR
                     PGGRLERVEGFR"
     gene            781..2997
                     /locus_tag="VF633_13130"
     CDS             781..2997
                     /locus_tag="VF633_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX8472046.1"
                     /translation="MTLYPIIMCGGAGTRLWPASRPSRPKQFIALAGNRSLFQDTVTR
                     VAALAGSEGKVVIIAGVSHQEAIETQLSEIAVEAQLLLEPEARDSAAAMAAAALWVQQ
                     RDPDGIIVFVASDHYVPDPAAFRTSVLEASGAAAEGRIVTLGVKPTEPSSAYGYIKPG
                     GTGLTAIISFHEKPQPDTARAFIQAGYLWNSGNFIVSARTLIEELAAHAPSVLAAARS
                     AVAPASNTSSLQILGEGFRAAPKISIDYAVMEPTKRASVLEVDFAWSDLGAWDAIAAS
                     GEGGVGVHIFEDSERILARAPDGKLIAALGVSDLAIIVEDDAVLVCDLSRSQEVKRLV
                     ERMRTISPRHLDFQKPAPETLEAGAQRFSSWLKLRALPVWSTLGFAADGGFSETLTLD
                     GQSTAGNRRARVQTRQIFVYAQAGALGWKGPWKSLIEAALDRLRTAYLRPDGATRALL
                     GPDWAVLDETAMLYDQAFVLFALAAARQANVDDAELENDAARLRDKLLSEALPNGAMR
                     EAGEHPFQSNAHMHLFEASLAWEALSEDPAWGAMADRIAGLAMDVFIDREGGFLREFF
                     DEAWNPAAGEDGRLFEPGHQFEWAWLLARYGLLRGDKRAAAAARALYASGRRGIGQRP
                     PVALDAMNEDMTIRSHRARLWPQTEWLKSSLILAETSTDGDRHVYLDDAATALRGLWL
                     YLTDNGVWRDKRFPDGTFLDEPAPASSFYHIIAAFDQLRRTFNALDLGEGRKLTLG"
     gene            complement(3018..>3579)
                     /locus_tag="VF633_13135"
     CDS             complement(3018..>3579)
                     /locus_tag="VF633_13135"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8472047.1"
                     /translation="HMVIVDVEGNAVSMTTTVESIFGSGRMVGGFFLNNQLTDFSFTP
                     TRADGGEAANAVAAGKRPRSSMSPVIILDQQGNLVGAMGSPGGSSILAYNAKALIGTL
                     VWGLPVQQAFNLPNLVAKGDGFGADTDRFSSALREGMDAAGIQIQPNASENSGLHGAL
                     WRRAADGIWGWDGGADDRREGQARTH"
BASE COUNT          625 a         1183 c         1125 g          646 t
ORIGIN      
        1 cgcgaaagcc ctcgacgcgc tccagacgac cgcccggacg cgccacgccc tgcccttcca
       61 gcacccgcgt cttgagacgg cccatctcgt cgggtccaaa cgtcagcggc ggctgcgcgc
      121 ccatcttgac gccggtccag ccgttctcgt tgtgcgaggc cgtgaccatg gccacgcacg
      181 gcgcgttcag cgcgaactgg ccgtagtagg ccatcggcga cagggccagg ccaatgtcca
      241 gcacctccat cccgccttcc agcagaccga tgatcagcgc ctgtttgacg ctcagcgaat
      301 aggaccggaa gtcgtggccg acgacaatgc gaggctggac gccgatctca tggacgtagg
      361 tcgccagcgc cagaccgagc gcctggatgc cgaggaggtt gatctctttc tcgagaatcc
      421 agcgcgcgtc gtactcccgg aacccggttg gcttcaccag cggcgtgatc tcgtagtcag
      481 ccgtgttcgg aaccagatcc tggcggggct tcatcataac ggggtcctga atggcgaggt
      541 ggaataggcg atcgatcatg caggctgcaa ccggccgcca tgttgacctc gactaaagtg
      601 aaggcgtgcc aaggtcaaaa tactgtttac tcaacgcctg agctgtcggg cacgattcgt
      661 agcggcctgc acctcgccga agatgtcagc cggcgaaagg atcacgagca ggatcgtgat
      721 acctgcggta tttcggctcg gcggatattc cctctcactc cggctcccgt ataagacggc
      781 atgaccctct atccgatcat aatgtgcggc ggcgccggaa cacgtctctg gccggcctcc
      841 cgcccatcgc ggcccaagca attcatcgct ctggccggca accggtcatt gttccaggac
      901 accgttacgc gcgtggctgc gctcgcaggg agcgagggca aggtcgtgat cattgcgggc
      961 gtatcccatc aggaagccat cgagacgcaa ctgtccgaga tcgctgtcga ggcgcaactc
     1021 ctgctggaac ccgaagcccg cgattcggca gctgccatgg cggctgcagc cctttgggtc
     1081 cagcagcgcg atcccgacgg gattatcgtc ttcgtcgcgt ctgaccacta tgtacctgat
     1141 cctgccgcct tccggacgtc cgttctcgaa gcatccggag ctgcggccga gggtcgcatc
     1201 gtgacgcttg gcgtgaagcc gaccgagccg tcgtcggcct acggttacat caagccgggc
     1261 ggaactggcc tgactgccat catctcgttc catgagaagc ctcaacctga tacggcgcgg
     1321 gccttcatcc aggcgggcta tctctggaac agtggcaatt tcatcgtatc ggccaggacg
     1381 ctgatcgagg aactggcagc ccatgcgcca tccgtactcg ccgcagcccg atctgcggtc
     1441 gcgccagcgt ccaatacgag cagcctgcag attctgggag aaggctttcg cgccgcgccc
     1501 aagatttcga tcgactatgc ggttatggag ccgaccaaaa gggcgtcggt tctggaagtc
     1561 gattttgcgt ggtcggatct gggcgcatgg gacgcgatcg ccgccagcgg cgagggcggc
     1621 gtcggcgtcc atatcttcga ggattccgaa cggatcctgg cccgggcgcc ggacggcaag
     1681 ctgatcgcag ctctgggcgt cagcgacctg gccatcatag tcgaggacga cgccgttctt
     1741 gtgtgtgacc tgtcgcgttc gcaggaggtc aagcggctag tggagcggat gcgcaccatt
     1801 tccccgcgtc acctggactt ccagaagcct gcacctgaaa ctctggaagc tggcgctcaa
     1861 cgcttttcga gctggctcaa gcttcgcgcc ctgccggtct ggtcgactct cggtttcgca
     1921 gccgacggcg gttttagcga gacccttacg ctggacggac agtccaccgc agggaacagg
     1981 cgcgcgcggg tccagacacg tcagattttc gtctatgccc aggctggagc ccttggctgg
     2041 aaggggccgt ggaagtcgct gatcgaggcc gcgctcgatc gtttgcgaac cgcctacctc
     2101 cgtcccgacg gcgcgacccg cgccctgctc ggccctgatt gggccgtgct cgacgagacg
     2161 gcaatgctgt acgaccaggc ctttgtcctg ttcgccctgg ccgccgccag acaggccaac
     2221 gtcgacgacg cggagctgga gaacgacgcc gcgagactac gcgacaaact gttatccgaa
     2281 gccttgccca acggcgccat gcgcgaggcc ggcgaacatc cctttcagtc caacgcccac
     2341 atgcatctgt tcgaggccag tctggcctgg gaggctttga gcgaagaccc ggcctggggc
     2401 gccatggccg accgtatcgc cggcttggcc atggacgttt tcatcgaccg cgaagggggt
     2461 ttcctgcggg agttcttcga tgaggcatgg aaccctgccg ccggcgagga cgggcggctc
     2521 ttcgagccgg gccatcaatt cgagtgggcc tggctgctgg cgcgctatgg cctgctgcgc
     2581 ggcgacaagc gcgccgccgc cgccgcccgg gcgctgtacg ccagcggccg caggggcatt
     2641 ggtcagcgac cgcctgtcgc tctcgacgcc atgaacgagg acatgaccat ccgcagtcat
     2701 cgcgcccggc tctggcccca gactgaatgg ttgaagtcgt cgttgattct ggcggagact
     2761 tcgactgacg gcgaccgaca tgtctatctc gacgacgccg cgaccgcgct gcgcgggctg
     2821 tggctctacc tgaccgacaa cggcgtctgg cgcgacaagc ggtttcccga tgggacgttc
     2881 ctcgacgaac cggcgcccgc gagttcgttc tatcacatca tcgccgcctt cgatcagttg
     2941 aggcgaacct tcaacgccct cgacctcggc gaggggcgca aactgacatt gggctgagct
     3001 ggttctgccc ggacgcctca gtgcgtccgc gcctgaccct cccggcggtc gtccgcaccg
     3061 ccgtcccagc cccagatacc gtcggcggca cgccgccaca gagcgccatg caggcctgag
     3121 ttctcggagg cgttgggctg gatctgaata ccggcggcgt ccatgccttc gcgcagggcc
     3181 gaggaaaacc gatccgtgtc cgcgccaaaa ccatcgccct tggccaccag gttgggcagg
     3241 ttgaacgcct gctgcaccgg caggccccag accagcgttc cgatcagcgc cttggcgtta
     3301 taggccagaa tcgaagagcc gccaggcgaa cccatggcgc cgaccaggtt gccttgttgg
     3361 tccaggatta tcaccggcga catcgacgac cgggggcgct tgcccgccgc cactgcattg
     3421 gccgcttcac cgccgtcggc tcgtgtcgga gtgaaggaga aatcggtcag ctggttgttg
     3481 aggaaaaagc cgccgaccat cctgcctgaa ccgaagatgc tctcgaccgt ggtggtcatg
     3541 ctgacggcat tcccctccac atcgacgatg accatgtgg
//
LOCUS       DASVTM010000476         7166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_100683,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000476 DASVTM010000000
VERSION     DASVTM010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100683"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="murD"
                     /locus_tag="VF633_13140"
     CDS             complement(<1..1152)
                     /gene="murD"
                     /locus_tag="VF633_13140"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX8472048.1"
                     /translation="MIAVPGFAGRRVAVFGLGRSGITAARALHAGGAIPVLWDDGVSG
                     RMQAEAEGFLVEDLTRADWNQFAALVLSPGAPLTHPKPHWTVEKARAAWVPIMGDIEL
                     FARALATVPEAVRPKVVAITGTNGKSTTTALIGWVLKQAGLKVAVGGNIGIGVLALPA
                     PVEVQIYVIEVSSYQLDLTSSFAPDVAILTNVTPDHLDRHGGMEGYVAAKRRLFQTAT
                     PESIALVGVDDDWGWSVVGDLNSRRLRVSTITTRTPGDRRESVAIDDIDAERPDQGPA
                     FDEGRFIIVDATDGHLTVDGIFVTELADARSLPGRHNAQNAAFAYAAARAVGVSNDDA
                     VAGLMSFPGLAHRMETVGQLGPVRFVNDSKATNADAARQALSSYPSVFWI"
     gene            complement(1157..2263)
                     /gene="mraY"
                     /locus_tag="VF633_13145"
     CDS             complement(1157..2263)
                     /gene="mraY"
                     /locus_tag="VF633_13145"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX8472049.1"
                     /translation="MFYLLYLHYADVANDYPLLHLIQYQTVRVALAMATAMIVAVAMG
                     SRFINWIRAKQGRGQPIRDDGPVSHLSKVGTPTMGGLMILAGIGVAVLLWGDLTNPYI
                     WIVSFVTAAFGLLGFIDDYAKITKQSSAGLTSKQKLVAQLVVSVIAGVATVLWMTPST
                     SPGLETSIAFPFFKDVLLNVGWFYVAFAAFTIIGFSNAVNLTDGLDGLAIVPVMMASA
                     AFGLISYLVGNFIFAEYLGVHHVPGSGELAIFCAAIIGGGTGFLWYNAPPAKIFMGDT
                     GSLALGGALGAIAVATKHELVLGIVGGLFVLEAASVMIQVGYYKLTKKRIFLMAPIHH
                     HFEKMGWPESTVVIRFWIVAAMLALLGLSTLKLR"
     gene            complement(2263..3699)
                     /gene="murF"
                     /locus_tag="VF633_13150"
     CDS             complement(2263..3699)
                     /gene="murF"
                     /locus_tag="VF633_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX8472050.1"
                     /translation="MPDSIQPLWTALDIAEATGGRIEGAAFDVHGITYNSREIAPGDL
                     FLALKGERDGHDFAAAAFAAGASGALVERPVEGGPCIVVADTLKALERLGVAARDRVP
                     QVLRGAVTGSVGKTSVTQAIKAGLDLAGPAHASIKSYNNHIGVPLTLSRMPADVERAV
                     FEIGMNAPGEIGPLSKMVQPHAACVTTVGPVHIEGFVDGEAGVAREKATIFQGLGPGG
                     VAVINGDNVWVDMLRETALHQGARIAQFGSGPGQDARLLDFVPGPDGARVTAEIWGRT
                     HAFSLKQTGFHWGLNSLAVILMLDALDVPVETAFAALAEFQPLAGRGETRQVRVSGGA
                     FTLIDESYNANPLSMRAGFMSLGAKPVAGGGRRIVVLTDMLELGNQATALHAGLAETI
                     EAAGLDLVHAAGPQMRHLYEALPSSRRGIWAETAADLAAQASALAGPHDIVMVKGSNG
                     SRASLVAKALAALDNAGHSAAQNAQGGV"
     gene            complement(3692..5149)
                     /locus_tag="VF633_13155"
     CDS             complement(3692..5149)
                     /locus_tag="VF633_13155"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX8472051.1"
                     /translation="MSAVRLSELLRRDVASDPVITGVTSDSRKVEPGGLFVALPGAVA
                     DGRAFIPQALAQGAAAVLAPTDTPADSAPLLVTSGDVRRAYAIAARSFYGAQPATCVA
                     VTGTNGKTSVAAFCRQIWAGLGLKSASMGTLGVVGQKGDKTYTLTGPGLTSLDAADAA
                     RLLAELASKQVTHLALEASSHGIDQRRLDGVSIKAAGFTNLTQDHLDYHGDMDGYRAA
                     KMRLFETLLPRGRTAVLNADSDAYSSFAAASIMSGLGIMGVGERGRDLALIGRKATPE
                     GQRLSIDVRGVVHDILLPLAGAFQASNALVAAGLCIAAGESADRVLAAMEHITGAQGR
                     LQRVLGGNRGGEVYVDYAHTPDGLETVLTALRPHAAGRLIVVFGAGGDRDRGKRSLMG
                     EIAGRLADVAIVTDDNPRSEMPAMIRAEIRKGCPDAREIGDRREAIQHAISLMRDGDV
                     VVIAGKGHEQGQIVGGFTHPFDDVTEATEALRVYA"
     gene            complement(5146..6897)
                     /locus_tag="VF633_13160"
     CDS             complement(5146..6897)
                     /locus_tag="VF633_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8472052.1"
                     /translation="MSVHDHRTPFPRPAPGPRAPLGSTVAPWLRIFASGIWWIEHAFE
                     RARADGRPEEDTRVRIFLITCLFSLVFVCLTLGAAHAGLFAKRGGVGSIDNPNALTRA
                     DLVDRNGALLASNITHYGLYIDPSQVWDRQLAYDQIRHALPAISASRLRRVLFGNRRL
                     IVQPGLTPRERALIHALALGGVSFEPEDRRVYPLNNSATYVVGTADTGGQGLSGAEQA
                     FNDEIRAAGVRGDEFPLSIDLRVQGVLENELAAAAMANGSRGAVGIVTDTRTGEVLGM
                     ASYPTNSLNRAVGSVYEMGSVFKTFTIAAAVDAGRADMNSMIDASSAYMIGSRRITDF
                     HATNRVLSLEEVYLHSSNIGSSKLAVGMGGPMMRDYMTRFGFLEKAPIELRESAKPIL
                     PRDWSDTTLASMSFGYGISITPAQMIGAMGALANGGRYLPLSLRKGGAPNRGEGRRVV
                     SEQTSLTMLELMRRNVVAGSGGLAEAPGLRVGGKTGSANKLVNGRYDPSHAVGTFAAI
                     FPADGPAGTQRYSILVLMDEPQTYPKTGGFVAAPAVGHIADRIAGVLGVQRRADAYRT
                     ALGDKVPVFQDVEGDGR"
     gene            complement(6894..>7166)
                     /locus_tag="VF633_13165"
     CDS             complement(6894..>7166)
                     /locus_tag="VF633_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEX8472053.1"
                     /translation="RESSQIDALNRDIAENRQRVRLLRAEISRLEQPSRLETLSREAG
                     LAPVDVHRQATEDQLPALKEAKAPALAAPAPKPAPVVVAEDPGAPL"
BASE COUNT         1244 a         2454 c         2318 g         1150 t
ORIGIN      
        1 gatccagaag acactcggat atgacgacaa cgcctgcctc gccgcgtcgg cgttggtcgc
       61 cttggagtcg ttgacgaacc ggaccggacc gagttgaccg accgtttcca ttcgatgggc
      121 cagaccgggg aagctcatca gccctgccac cgcgtcatcg ttggacacgc ccacagcccg
      181 tgcggcggca taggcgaagg ccgcgttctg ggcgttgtgc cgtccgggca gcgaccgcgc
      241 gtcggccagt tcggtgacga agatgccgtc gaccgtcaga tggccgtcgg tggcgtcgac
      301 gatgatgaac cgcccttcgt cgaaggcggg gccctgatcc ggccgttcgg cgtcgatgtc
      361 atcgatcgcg acgctctcgc gtcggtcgcc cggtgtgcgc gtggtgatcg tgctgacccg
      421 cagccgtcgc gagttcaggt cgccaacgac gctccagccc cagtcgtcat ccaccccgac
      481 cagcgcgatg ctctcgggcg tcgcggtctg gaacagccgc cgcttggccg cgacatagcc
      541 ctccatcccg ccatgccgat cgagatggtc gggcgtgacg ttggtcaaga tcgccacgtc
      601 gggcgcgaag ctgctggtca ggtccagctg atagctcgag acctcaatga catagatctg
      661 cacctcgacc ggcgccggca gggccaagac gccgatgccg atattgccgc cgacggcgac
      721 cttcagccca gcctgtttca gcacccagcc gatcagggcc gtggtcgtgg acttgccgtt
      781 ggtgccggtg atggcgacca ccttgggccg caccgcctcg ggtacggtcg ccagcgcgcg
      841 ggcgaacagt tcgatatcgc ccatgatcgg cacccaggcc gccctggcct tctcgaccgt
      901 ccagtgcggc ttgggatggg tcagcggcgc gccgggcgac agcaccagcg cggcgaattg
      961 gttccagtcg gcgcgggtca ggtcctcgac gaggaagcct tccgcctcgg cctgcatccg
     1021 gcccgagacc ccgtcgtccc acaggaccgg gatcgcgccg cctgcatgca gcgctcgcgc
     1081 cgctgtgatc cccgaccttc cgaggccgaa gaccgcgacg cgccgcccag caaatcccgg
     1141 gaccgcgatc atggcgctat cgaagtttca gcgtggacag gccgagcagg gccagcatcg
     1201 ccgccacgat ccagaaccgg atcaccacgg tcgattccgg ccaccccatc ttctcgaaat
     1261 ggtggtggat cggcgccatc aggaagatgc gcttcttggt cagcttgtaa tagccgacct
     1321 ggatcatgac cgaggccgct tccagcacga acaggccgcc gacgatgccc agcaccagtt
     1381 catgcttggt cgccaccgcg atggcgccga gcgcgccgcc cagagccagc gagcctgtat
     1441 cgcccatgaa gatcttcgcg ggaggcgcgt tgtaccagag gaagcccgtg ccccccccga
     1501 tgatggcggc gcagaagatg gccagttcac ccgagcccgg cacatggtgg acgcccaggt
     1561 attcggcgaa gatgaagttg ccgacgagat acgagatcag tccgaacgcc gcagaagcca
     1621 tcatcaccgg cacgatcgcc agcccgtcca gaccgtcggt caggttcacc gcgttggaaa
     1681 agccgatgat cgtgaaggcg gcgaacgcga cgtagaacca gcccacgttc agcagcacgt
     1741 ccttgaagaa gggaaacgcg atcgacgtct ccagacccgg cgaggtcgaa ggcgtcatcc
     1801 agagcaccgt agcgacaccc gcgatcaccg aaaccaccag ttgcgccacc aacttctgct
     1861 tcgaagtcag gcccgccgag ctctgtttcg tgatcttggc gtagtcgtcg atgaaaccca
     1921 gcaaaccgaa ggcggccgtc acgaagctga ctatccagat gtagggattg gtcaggtcgc
     1981 cccacagcag cacggcgacg ccgatgccgg cgaggatcat cagtccgccc atggtcgggg
     2041 tgccgacctt ggacaggtgc gagaccgggc cgtcgtcccg gatcggctgg ccacggccct
     2101 gtttggcgcg gatccagttg atgaaccgcg agcccatggc caccgccacg atcatcgccg
     2161 tggccatcgc cagcgccacc cgtactgtct gatactggat cagatgaagc agcgggtagt
     2221 cgttcgccac gtcggcgtag tgaagataga gcaggtaaaa catcagacgc ccccttgagc
     2281 gttttgggcg gcgctgtggc ccgcattatc gagcgcagcg agcgccttgg ccactagcga
     2341 ggccctggac ccgttggagc ccttgaccat gacgatgtca tggggacccg ccagcgcgct
     2401 cgcctgggcg gcgagatcgg cggccgtctc ggcccagatc ccccgtcgcg aagacggcaa
     2461 cgcctcgtac aggtggcgca tctgcggacc ggcggcatgg accagatcca gccctgcggc
     2521 ctcgatggtc tcggccaacc cggcatgcag cgccgtcgcc tgattgccga gctcgagcat
     2581 gtcggtcagg acgacgatgc gacgcccgcc gcccgccacg ggtttggcgc cgaggctcat
     2641 gaagcccgcc cgcatcgaca gcggattggc gttgtagctt tcgtcgatca gggtgaatgc
     2701 gccaccggac acgcggacct gacgcgtctc gccccgcccg gccagaggct ggaactcggc
     2761 cagcgccgcg aacgccgtct cgaccggcac gtccagcgcg tccagcatca ggatgaccgc
     2821 aaggctgttc agcccccagt ggaagcctgt ctgtttcagc gaaaacgcat gggtccgccc
     2881 ccagatctcg gcggtcactc gtgcgccgtc cggccccggc acgaagtcca gcaaccgcgc
     2941 atcctgaccc ggccccgagc cgaactgtgc gatcctcgca ccctgatgca acgccgtttc
     3001 acgcagcatg tcgacccaga cgttgtcgcc gttgataacc gccacgccgc cgggcccaag
     3061 gccctgaaag atggtcgcct tctcgcgcgc gacccctgcc tcaccgtcga cgaagccttc
     3121 gatatgcaca ggaccgacgg tggtgacaca ggccgcatgc ggctggacca tcttcgatag
     3181 cggcccgatc tcgcccggcg cattcatgcc gatctcgaac accgcccgtt cgacgtctgc
     3241 aggcatccgc gacagcgtca gcggcacacc gatatggttg ttgtagctct tgatcgacgc
     3301 atgcgcggga cctgccaaat cgagccccgc cttgatcgct tgcgtgacgc tggtcttgcc
     3361 gacgctgccg gtgactgcac ctcgcagaac ctggggcacg cggtcccggg ctgcgacgcc
     3421 aagcctctcc agcgccttga gtgtgtccgc cacgacgatg cacggaccgc cctcaacagg
     3481 ccgttcgacc agcgctccgg aggctcctgc agcgaaggcg gcggcggcga agtcgtgccc
     3541 gtcgcgttcg cccttcaacg ccaggaacag gtcgcccggc gcaatctcac ggctgttgta
     3601 ggtgatgccg tggacgtcga aggccgcgcc ctcgatccga ccgccggtgg cctcggcgat
     3661 atcgagcgcc gtccacagcg gctggattga atcaggcata gacgcgcagg gcctcggtcg
     3721 cttcggtgac gtcgtcgaac ggatgagtga acccgccgac gatctgaccc tgttcatgcc
     3781 cttttccggc gatgaccacc acatccccgt cgcgcatcag cgaaatggcg tgctggatcg
     3841 cttcgcgacg gtcgccgatt tcgcgggcgt cgggacagcc tttgcggatc tcggcccgga
     3901 tcatggcggg catttccgag cgcgggttgt cgtcggtgac gatggccacg tcggccaatc
     3961 gtcccgcgat ctcgcccatc aacgagcgct tgccccggtc ccggtcgcca ccggcgccga
     4021 acaccacgat cagtcgaccc gccgcatggg gacgcaatgc cgtcagcact gtttccagtc
     4081 cgtcaggggt atgggcgtaa tcgacataaa cctcgccgcc ccgattgccg cccagcactc
     4141 gctgcagacg gccttgcgcg cccgtgatgt gttccatcgc cgcgagcacc cggtcagcgc
     4201 tctcgccggc cgcgatgcac agcccggctg cgaccaaggc atttgaagcc tgaaaggcgc
     4261 ccgccaacgg cagcagtata tcgtggacca cgccgcgcac gtcgatcgac agccgctggc
     4321 cttcgggcgt cgccttgcgg ccgatcagcg ccagatcgcg gccccgctcg cccacgccca
     4381 tgatgccgag accggacatg atgctggctg ccgcgaagct ggaataggcg tcagagtccg
     4441 cattgagcac tgccgtccgc cctcggggca gcagcgtctc gaacagcctc attttggctg
     4501 cccgatagcc gtccatgtcg ccatgatagt caaggtggtc ctgggtcagg ttcgtgaacc
     4561 cggccgcctt gatcgacacg ccgtcgaggc gtctctggtc aatgccgtgc gaagaggctt
     4621 ccagcgccag gtgcgtcact tgtttcgagg ccagttccgc caacaggcgt gccgcgtccg
     4681 ccgcgtcaag gctggtcaga ccggggccag tcagagtgta ggtcttgtcg cccttctggc
     4741 cgacgacgcc aagcgtgccc atgctggccg acttgaggcc caggccggcc cagatctggc
     4801 ggcagaaggc ggcgaccgag gtcttcccgt tagtgccggt gacggccacg caggttgcgg
     4861 gctgcgcacc gtagaagctg cgcgctgcaa ttgcataggc gcgacgtacg tcgcccgaag
     4921 tcaccagcag cggcgcagaa tcggcgggcg tgtcggtggg ggccagaacc gcagcggcgc
     4981 cctgggccag cgcttgcgga ataaaggcgc ggccgtcggc aactgcgcca ggcagggcca
     5041 cgaacaggcc gcccggctca accttgcggc tgtccgaagt gactccggtg atgaccgggt
     5101 cggaggcaac atcccggcgc aacagttcag atagacggac agcgctcatc ggccgtcgcc
     5161 ttccacgtcc tggaacaccg ggaccttgtc gcccagagca gtgcggtagg cgtcggcgcg
     5221 gcgctggacg ccgaggacgc ccgcgatcct gtcggctata tgcccgaccg caggcgccgc
     5281 tacgaaacca ccggtcttgg gataggtctg gggctcgtcc atcagcacaa ggatcgaata
     5341 gcgctgcgtg ccggccgggc catcggcggg gaagatggcc gcaaaggtgc cgacggcgtg
     5401 gctcggatca tagcggccgt tgaccagctt gttcgccgac ccggtcttgc cgccgacgcg
     5461 caggcccgga gcctcggcaa ggccgcccga cccggcgacg acgttgcggc gcatcagttc
     5521 cagcatcgtc agcgacgttt gttccgacac cacgcggcgg ccttcgcccc ggttcggagc
     5581 cccgcccttg cgcaacgaca gcggcagata acggcctccg ttggccagcg cacccatggc
     5641 gccgatcatc tgggccggtg tgatggagat gccgtaaccg aaggacatcg aggccagcgt
     5701 cgtgtccgac cagtcgcgcg gcaggatcgg cttggccgat tccctgagct cgatcggcgc
     5761 cttctcgagg aagccgaagc gggtcatgta gtcccgcatc atcggcccgc ccatgccgac
     5821 cgccagcttc gaactgccga tgttggacga gtgcagatag acttcttcga ggctcagcac
     5881 gcgattggtg gcgtggaagt cggtgatccg ccgcgacccg atcatatagg cgctcgaggc
     5941 gtcgatcatc gagttcatgt cggcccgccc cgcatcgacg gccgcggcga tggtgaaggt
     6001 cttgaacacc gagcccatct cgtaaaccga gcccacggcg cggttcaatg agttggtcgg
     6061 atagctggcc atgccgagca cttcgccggt gcgggtgtcg gtgacgatgc cgaccgcgcc
     6121 tctggacccg ttcgccatgg cggccgcggc aagttcgttt tcgagcacgc cttgaacccg
     6181 caggtcgatc gacagcggaa actcgtcgcc acgaacgccg gcagcccgga tttcgtcgtt
     6241 gaaggcctgc tctgcgcccg acagaccttg cccgccggta tcggcagtgc cgacgacgta
     6301 ggtcgcggag ttgttcagcg gatagactcg acgatcctcc ggctcgaacg acacaccgcc
     6361 cagagccaaa gcgtggatca acgcccgctc gcggggggtc aggcccggct gcacaatcag
     6421 ccgccggttg ccgaacagca ccctgcgtaa ccggctggcg gaaatggccg gcaaggcatg
     6481 gcggatctgg tcataagcca actgccggtc ccatacctga gacgggtcga tatagaggcc
     6541 gtaatgggtg atgttcgacg ccagcagcgc gccgttgcgg tccaccagat cggcgcgggt
     6601 cagggcgttc ggattgtcta ttgagccgac gcccccgcgc ttggcgaaca gccccgcatg
     6661 cgccgcaccg agcgtcaggc aaacgaacac cagcgagaag aggcaggtga tgaggaagat
     6721 gcggacacgt gtatcctctt ccggtcggcc gtcggcgcga gcacgttcga acgcatgctc
     6781 aatccaccag atgccggatg cgaaaatccg tagccacggc gctaccgtcg atcccagcgg
     6841 cgcgcgcggg cccggggccg gacgaggaaa cggtgtgcgg tggtcgtgaa cgctcacagc
     6901 ggcgctcccg gatcttcagc gacgacaacc ggcgcaggct tgggcgcagg cgccgcgaga
     6961 gccggagcct tggcctcttt cagcgcaggc aactgatcct cggtcgcctg gcggtgcaca
     7021 tcgaccggcg ccagcccggc ctcgcgtgac agcgtttcca gacggcttgg ctgctccaga
     7081 cgggaaatct cggcccgaag cagtcggacc cgctggcgat tttcggcgat atcccggttc
     7141 agtgcgtcga tctggctgct ctcgcg
//
LOCUS       DASVTM010000477         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_469321,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000477 DASVTM010000000
VERSION     DASVTM010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469321"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VF633_13170"
     CDS             <1..1076
                     /locus_tag="VF633_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8472054.1"
                     /translation="SVLETPPTTDRSWDAVWSWWDTNPHSYWWHRGKYVHERALPPKK
                     TRQLIYAFSRLREIHHDKGDFLVNYIDADSAPPVDHFKAGATGMGRYDVLQARNVAGN
                     LNASLAASWHAFDHMAWDGLKYPHLSANGTHPYWVLGKGLFFRSSDLYELGGFHPWIA
                     IEDPEVGMRFWASGKRLGVIKGSLIEEVPETFSEGITQRKRWVCGFFQSLGHPLTELG
                     FSPADKIKAWLNFLPCLSLWINAVGFPVGVWAIAAWLDDADVIPTWLVWLCAINLACF
                     VVSLGSLYISTWRRTRLVLDKTSDRIGYMLRINPLFILVWWIVWLIPLGLGFWMAIRE
                     GGLVWERTRKVNANESLIHSQAR"
     gene            complement(1083..2066)
                     /locus_tag="VF633_13175"
     CDS             complement(1083..2066)
                     /locus_tag="VF633_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX8472055.1"
                     /translation="MTLSLDDAARSRQRYFGSAVRFDQLEQEPELRRRLLADCGSVTP
                     EVHMKWDALNPSPDDWTFEGADDLATFAAANALKLRGHTLLWDQSTPAWAKTAMIRTH
                     DWALVAQFIRTVVARYADHVDEWDVVNEPIDTDGLRANTFLRAYGPAYVERSLHEARA
                     ASARPRLLINDYGFDYDNPVEEGRRRSFLRLLEDLKTRGAPLDGVGVQAHLDLTKGPL
                     RPEILRPFFAEIAGMGLSIDISELDVKEGDISRPTAERDRLVADEVRRYLDIALDEPA
                     VRGVTTWGLSDRHSWLKAEGATGAALNRGLPYDDNLQPKPMRDALRSQLST"
     gene            2323..>2967
                     /gene="bcsS"
                     /locus_tag="VF633_13180"
     CDS             2323..>2967
                     /gene="bcsS"
                     /locus_tag="VF633_13180"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HEX8472056.1"
                     /translation="MQKSSKQRALSGVAAAIPALALAFSVGSAFAQSAGAVFAGGETG
                     RDWSAYAGAVWALEMGGDARWAVRGIASTGGYDYRSSSVEVESKYGQAEVVLLRQTSG
                     AWGYLNVGGGARFTDTDLEPDDPGNGRRGGRWDALIAADGVRRIQDWEVGGFATYGVD
                     MEEYFVRGRVTRGVGTNGLRLGVETVVQGDPNYDRQGVALVAMHRQDSGLEWTAT"
BASE COUNT          512 a          908 c          983 g          564 t
ORIGIN      
        1 tctccgtgct cgaaacgccg ccgacgaccg accggagctg ggatgcggtc tggtcctggt
       61 gggacacgaa cccccattcc tactggtggc accgcggcaa atacgtccac gagcgtgcgc
      121 tgccgccgaa gaagacccgg cagttgatct acgccttctc tcgcctgcgc gaaattcatc
      181 acgacaaggg tgactttctc gtcaactata tcgacgctga cagcgccccg ccggtggacc
      241 acttcaaagc gggagcgaca ggaatgggcc gctacgatgt cctgcaggcc cggaacgtgg
      301 cgggcaatct gaacgcgagc ctcgccgcca gctggcatgc cttcgaccat atggcctggg
      361 acggattgaa gtatcctcat ctttccgcca acgggaccca cccctactgg gtcctgggca
      421 agggattgtt cttccgctcg agcgaccttt atgaactcgg cggcttccat ccgtggattg
      481 ccatcgagga tccggaggtt ggcatgcgtt tctgggcgtc cggcaagcgg ctgggcgtga
      541 tcaaaggctc cttgatcgaa gaggtgcccg agaccttctc cgaagggatc acccaaagaa
      601 aacgctgggt ctgcggcttt ttccagagcc tcggccatcc gttgaccgaa ctgggattca
      661 gcccggccga taagatcaag gcgtggctga acttcctccc ttgcctgtcc ttgtggatca
      721 acgcagtggg ttttccggtc ggggtgtggg ccattgcggc ctggctcgac gatgccgacg
      781 tcatcccgac atggctggtg tggctctgcg cgatcaacct cgcctgtttc gtcgtctcac
      841 tggggagcct ctacatcagt acgtggcggc gaacgcgcct ggttctggac aagacatccg
      901 atcggatcgg ctacatgctc cgcatcaatc ctctgtttat tctggtctgg tggatcgtct
      961 ggctgatccc gctagggctc ggcttttgga tggcgatccg cgagggcggc ctggtctggg
     1021 agaggacacg caaggtcaac gcgaacgagt cccttatcca ctcccaggcc cggtgacggg
     1081 cgtcaggtgg aaagctgcga gcgcagggcg tcccgcatgg gcttgggttg cagattgtcg
     1141 tcgtagggaa gtccccggtt cagggcggcg cccgtggctc cctcggcctt cagccaggag
     1201 tgccggtcgc tgagccccca ggtggtgacg ccgcgcacgg ccggttcgtc gagcgcgatg
     1261 tcaagatagc gccggacctc gtcggcgacg agtctgtccc gctcggccgt agggcggcta
     1321 atgtccccct ccttcacgtc gagctcagag atgtcgatgc tcagccccat gcccgcgatc
     1381 tcggcgaaga aggggcgtag gatttcgggt cgcagcgggc ccttcgtgag atcgaggtga
     1441 gcctgaacgc cgacgccatc cagcggcgcg cctctggtct tgaggtcttc cagcagtcgc
     1501 aaaaaggacc gacgcctgcc ctcctcgacc ggattgtcgt agtcgaagcc gtagtcgttg
     1561 atcagcagcc ggggccgggc gctggccgcg cgcgcctcat gaagcgaacg ctcgacatac
     1621 gcagggccgt aggcgcgcaa gaaggtattg gccctcagac catcggtgtc gatcggttca
     1681 ttgacgacat cccactcatc gacgtggtct gcataacgcg cgacgacggt acggatgaac
     1741 tgtgccacga gcgcccagtc atgggtcctg atcatcgccg tcttggccca ggccggcgtg
     1801 ctttggtccc aaagcagggt atggcctctc agcttaagag cgttcgcggc ggcgaaggtc
     1861 gccaggtcgt ccgccccttc aaaggtccag tcatcgggcg acgggttcag cgcgtcccat
     1921 ttcatgtgaa cctctggcgt gaccgagccg cagtccgcca gaagcctgcg ccgcaattcc
     1981 ggctcctgct ccagctggtc gaagcgtact gccgagccga aataccgttg ccgcgacctc
     2041 gcggcgtcgt cgagcgacag ggtcatctca ccctccgctg gattggcggc cagaaagggt
     2101 gctgaggcca agcctatgag aagtgcgcga cggttcacta gacggattcc gcatcctgtg
     2161 cgtgttcgag agaaaatcct ggaccctcaa cgttaacttt tgatgcatgg gcgtcacggc
     2221 gaaattgcga caatctatgt caattgaaag agatggcgcg gatcgcgcct gtcagcgtgg
     2281 cggctcgcct tgagggcgcg acaaccgcta cgggaggctg atttgcagaa aagttccaaa
     2341 cagcgggcct tatccggcgt cgccgcggcg atcccggccc ttgccctggc gttctcggtc
     2401 ggctccgcat ttgcacaatc cgctggcgcc gttttcgcgg ggggcgagac gggccgtgac
     2461 tggtcggcct acgccggcgc cgtgtgggcg ctggagatgg gcggggacgc ccgatgggca
     2521 gttcgcggca tcgcatcgac cggaggctac gactaccgaa gttccagcgt ggaggtcgag
     2581 tcgaagtacg gccaggctga ggtcgtgctc ctgcgccaga cgtcgggcgc ttgggggtat
     2641 ctcaacgtcg ggggaggtgc gcgcttcacc gatacggacc tggaaccgga cgatccaggc
     2701 aacgggcggc gtggcggacg atgggacgcg ctgatcgccg cagacggtgt gcgacggata
     2761 caggactggg aggtcggagg tttcgcgacc tacggcgtcg atatggagga atatttcgtt
     2821 cggggccgtg tgacccgcgg cgtcgggacg aacggcctgc ggctcggcgt cgagactgtc
     2881 gtccagggcg atccaaacta cgatcgccaa ggcgtcgccc tggtcgccat gcaccggcag
     2941 gattcgggtc tggaatggac cgcgacg
//
LOCUS       DASVTM010000478         4300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_738343,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000478 DASVTM010000000
VERSION     DASVTM010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738343"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VF633_13185"
     CDS             <1..600
                     /locus_tag="VF633_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8472057.1"
                     /translation="YGVAGWLPLFFMRSFDLTLAQTSWYYSGIALIGGLLGIWLGGSI
                     ADKLAKRGKGAYPLTPAIAFIISAPCFLAAMNSPWLIGLVLPGGGSNAQQLILAFLIF
                     LIPTGLNLAWLGPITAAVQHIAPAPMRSTASAIFLLFNNLLGIAVGFFYFGWMSDLLA
                     PTFGAESLRWSIYTGMGFYLLASALLFGASRTLKKDWVD"
     gene            726..1916
                     /gene="sucC"
                     /locus_tag="VF633_13190"
     CDS             726..1916
                     /gene="sucC"
                     /locus_tag="VF633_13190"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX8472058.1"
                     /translation="MNIHEYQAKQVLKSFGAPVAEGVAVTSVDQVEAAAKSLPGPLYV
                     VKSQIHAGGRGKGKFKELGPDAKGGVRLAFSVEEAVSHAKEMLGNTLVTAQTGDAGKQ
                     VNRLYIEDGADIERELYCSLLVDRAHGRIAYVVSTEGGMDIEAVAHDTPDKIQTIVID
                     PETGVTEADVAAINSAYGLTGDAAEDGKTLFPILYKAFVEKDMDMLEVNPLIVMKDGH
                     LRVLDAKVSFDGNALFRHDDMKALRDETEEDAKEIEASKWDLAYVSLDGNIGCMVNGA
                     GLAMATMDIIKLYGKEPANFCDVGGGAGKDKVAAAFKIITADPNVQGILVNIFGGIMK
                     CDVIAEGVVAAVKEVGLKVPLVVRLEGTNAELGKKILNESGLAITAADDLDDAAQKIV
                     AAVG"
     gene            1919..2359
                     /locus_tag="VF633_13195"
     CDS             1919..2359
                     /locus_tag="VF633_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472059.1"
                     /translation="MLISLILSAALQATAPADAIGEFRALCLATAGDANRVAATVIER
                     GGWNTPARTGDLFIWSRLDDPTTTLITGVQQGRVVCMLKKPEIVNVEAALAAGLGPHF
                     GAVGDSGLHVFAPSAEAFAARDLTFIQTANADGHTTLTAIRPPR"
     gene            2683..3027
                     /locus_tag="VF633_13200"
     CDS             2683..3027
                     /locus_tag="VF633_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472060.1"
                     /translation="MKSNATDVAFVVTGRITREIGGKTVVLEFCGVTARPTDHRALER
                     RLRELMNFPPVRGGGLDAYAWLQTPEGRAPARSLGDAQFVAMAETGQMRMLGIDRSGR
                     GSTLIYFLPRLD"
     gene            3072..3329
                     /locus_tag="VF633_13205"
     CDS             3072..3329
                     /locus_tag="VF633_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HEX8472061.1"
                     /translation="MTRNLTLAIDDDLLDKVRVLAAMKRTSVNEMVRGFLARLVEQES
                     QHDEATEALLKLARESKGRMGEWRFSREETYSGEPRFDREY"
     gene            3313..3735
                     /locus_tag="VF633_13210"
     CDS             3313..3735
                     /locus_tag="VF633_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX8472062.1"
                     /translation="MIASTEVFLDTNVLLYAAMGSVDDPAKNERAIELLTTRFGTSGQ
                     VLAEFYVNAQRKGSTPLTADEAKEWVFRLSKKAFQPVDYSVIRGGIEHARRYQLSYWD
                     GAIIAAAERLGAKILYSEDLNHGQTYGSVRVENPFLPA"
     gene            3754..>4300
                     /locus_tag="VF633_13215"
     CDS             3754..>4300
                     /locus_tag="VF633_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX8472063.1"
                     /translation="MSILVNKNTKIIVQGLTGKTGGFHTEQALAYHGTQMVAGVHPTK
                     GGTNWTGSHGESLPIYASVAEAKDATGADASVIYVPPSGAAAAIIEAIDAEIPFITCI
                     TEGIPVLDMVKVKRHLEGSKSRLLGPNCPGILTPDECKIGIMPGSIFKKGSVGIVSRS
                     GTLTYEAVFQTTNEGLGQTTAV"
BASE COUNT          825 a         1425 c         1285 g          765 t
ORIGIN      
        1 tacggcgtcg ccggctggtt gccgctgttc ttcatgcgca gcttcgacct gacgctggcc
       61 cagacaagct ggtactattc cggcatcgcc ttgatcggcg gtctgctggg catctggctc
      121 ggcgggtcta tcgccgacaa gctggccaag cgcggcaagg gcgcctatcc gctgaccccg
      181 gccatcgcct tcatcatctc tgcgccctgc ttcctggcgg cgatgaactc gccctggctg
      241 atcggtctgg tgttgccggg cggcggatcg aacgctcagc agctgatcct cgccttcctg
      301 atcttcctga tcccgacggg tctgaacctg gcatggctgg gtccgatcac ggcggcggtc
      361 cagcatatcg ctccggcgcc catgcgctcg accgcttcgg ccatcttcct gctgttcaac
      421 aacctgctgg ggatcgccgt cggcttcttc tatttcggct ggatgagcga cctgctggct
      481 ccgaccttcg gcgccgaaag cctgcgctgg tcgatctata ccggcatggg cttctacctt
      541 ctggcctcgg ccctgctgtt cggagcgtcg cgcacgctga agaaggattg ggtcgactga
      601 tgcggctgcg gacccgatcc atagcctaac tgtcgatcac agacggttgc acgtgcgaac
      661 gggcgacgcc tataagcgcg gccaactctt ccccaaggcc acactcgcca caggacgtcg
      721 gatccatgaa cattcacgaa tatcaggcca agcaggtcct caagagcttt ggcgcgccgg
      781 tcgccgaggg cgtcgccgtc acctcggtcg atcaggtcga ggccgctgca aagtccctgc
      841 cgggtccgct ctatgtcgtg aagtcgcaga tccatgccgg cgggcgcggc aagggcaagt
      901 tcaaggaact cggccctgac gccaagggcg gcgttcgcct ggccttctcg gtcgaggaag
      961 ccgtctctca cgcaaaggaa atgctcggca acacgctggt caccgcccag acgggcgacg
     1021 ctggcaaaca ggtcaaccgc ctctatatcg aagatggcgc cgacatcgaa cgtgagctgt
     1081 attgttcgct gctggtcgac cgcgctcacg gccgtatcgc ctacgtcgtc tcgaccgaag
     1141 gcggcatgga catcgaagcc gtcgctcacg acacgcccga caagatccag accatcgtca
     1201 tcgacccgga aacaggcgtg accgaggctg acgtggccgc catcaactcc gcctacggcc
     1261 tgaccggcga cgccgccgaa gacggcaaga cgctgttccc catcctgtac aaagccttcg
     1321 tcgagaagga catggacatg ctggaggtga acccgctcat cgtcatgaag gacggccacc
     1381 tgcgcgtcct cgacgccaag gtctcgttcg acggcaacgc cctgttccgt catgacgaca
     1441 tgaaggccct gcgcgacgaa accgaggaag acgccaagga aatcgaagcc tccaagtggg
     1501 acctcgccta cgtctcgctc gacggcaata tcggctgcat ggtcaatggc gccggcctcg
     1561 ccatggcgac gatggacatc atcaagctgt acggcaaaga gcctgccaac ttctgcgacg
     1621 tcggcggcgg cgccggcaag gacaaggtcg cggcggcgtt caagatcatc accgccgacc
     1681 ccaacgtcca gggcatcctg gtcaacatct tcggcggcat catgaagtgt gacgtcatcg
     1741 ccgaaggcgt ggtcgcggcc gtaaaggaag tgggcctcaa ggttccgctc gtcgtccgcc
     1801 ttgaaggcac caacgccgaa ctgggcaaga aaatcctcaa cgagtccggc ctcgccatca
     1861 cggcggcgga cgacctcgac gacgccgccc agaagatcgt cgcggcggtc ggctagaggt
     1921 gctgatctcc ctgatcctgt cggcggcctt gcaagcgact gcacccgccg atgcgatcgg
     1981 ggagtttcgc gccctgtgcc ttgctacggc cggtgacgcc aatcgcgttg ccgccacggt
     2041 catagaacga ggcggctgga acacccctgc ccgaaccggg gacctgttca tctggtcccg
     2101 actggatgat ccaacgacga cattgatcac cggtgtgcaa cagggccggg tcgtctgtat
     2161 gttgaaaaag cctgagatcg taaacgtgga agcggccctc gccgccgggc tcggacccca
     2221 tttcggcgcg gtcggcgaca gcggactcca cgttttcgcg ccctcggccg aggccttcgc
     2281 ggcgcgcgac ctgacgttca tccagactgc gaatgccgac ggccacacga ccctgacagc
     2341 aatccgtccg ccgcgctgag gccgccagag ttacctcggc gctcgccggg gtagaacatt
     2401 gactcagccg caaccctgct gaacatagca gccatggtcg ctcaacgagt tattcgagtc
     2461 gcagcggcat tgtccgtcct ggtgttggca gcttggcctg tctgggctca gaccccagcg
     2521 ctgcccgagc ccatcgatct gatcgcaacc ctgaacacgg tgtgcaccag agctcagggt
     2581 gaccgaagcc gggcggccgc gctggcggca gaagccggtt tttcgccggt ccccgcttcg
     2641 atgcttcccc gcatacgcaa cagcagcgag accaccggtt tcatgaaatc gaacgcgacc
     2701 gatgtcgcat tcgtcgtaac tggcaggatc acccgcgaga tcggcggcaa gacggtggtg
     2761 ctcgaatttt gtggtgtcac cgccagacca accgatcacc gcgctctgga acgccggttg
     2821 cgcgagctga tgaacttccc cccggtgcgc ggtgggggac tcgacgccta tgcctggctg
     2881 cagacgcccg aaggtcgagc gcccgcgcgc agtctcggtg acgcccagtt cgtcgccatg
     2941 gctgagacag gccagatgcg gatgcttggc atagaccgct caggtcgggg ttcgacactg
     3001 atctatttcc ttccgcgcct cgactgacgt gcagccacag gctatgtgct acacataacc
     3061 cgtgtagatt catgacccgc aacctcaccc tcgccataga cgacgacctg ctcgacaagg
     3121 ttcgtgttct ggccgccatg aagcggacca gcgtcaacga gatggtgcgc ggctttctgg
     3181 cccgacttgt ggagcaggaa agccaacacg acgaagccac cgaggcgctg cttaaattgg
     3241 cccgcgagag caaaggccgt atgggcgaat ggcgcttcag ccgcgaagag acctacagcg
     3301 gagaacctcg gtttgatcgc gagtactgag gtttttctgg atactaacgt cctgctctat
     3361 gccgccatgg gttctgtcga tgacccggcc aagaacgagc gagctattga gctgttgacg
     3421 acccgttttg gtacttcggg ccaagtgctg gctgagttct acgtcaacgc ccagcgcaag
     3481 ggctccaccc ctcttactgc cgatgaggcc aaggagtggg tgttccgact gtccaagaag
     3541 gcgttccagc cggtggacta ttcggtgatc cgaggcggta tcgagcacgc ccggcgctat
     3601 caactatcct actgggatgg agcaatcatc gccgccgccg aacggctcgg tgcgaaaatc
     3661 ctctactccg aagatctcaa ccacggtcag acctacgggt cggtccgcgt cgaaaaccct
     3721 ttccttcccg cctaacccaa agaagcgagc ctcatgtcca ttctcgtaaa caaaaacacc
     3781 aagatcatcg ttcagggcct gaccggcaag accggcggct tccacaccga acaggcgctg
     3841 gcctatcacg gcacgcaaat ggtcgccggc gtccacccga ccaagggcgg cacaaactgg
     3901 accggatcgc acggcgaaag cctgccgatc tacgcgtcgg tcgccgaagc caaggacgcc
     3961 acgggcgctg acgcctcggt gatctacgtc ccgccgtcgg gcgccgccgc cgcgatcatc
     4021 gaagcgatcg acgccgagat tcccttcatc acctgcatca ccgaaggcat cccggtgctg
     4081 gacatggtca aggtcaagcg tcacctcgaa ggttccaagt cgcgcctgct gggcccgaac
     4141 tgccccggca tcctgacccc ggacgagtgc aagatcggca tcatgccggg ctccatcttc
     4201 aaaaagggct cggtcggcat tgtgtcgcgc tccggcaccc tgacctatga agccgtgttc
     4261 cagacgacca acgaaggcct cggccagacg acggccgtcg
//
LOCUS       DASVTM010000479         9330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_632226,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000479 DASVTM010000000
VERSION     DASVTM010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632226"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..612)
                     /locus_tag="VF633_13220"
     CDS             complement(52..612)
                     /locus_tag="VF633_13220"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX8472064.1"
                     /translation="MSSDIADRLGAEAPKVAASLIGASLTVDGVGGIIVETEAYAALD
                     PASHSFRGRTPRNAAMFGPVGRTYVYRIYGLHWCLNVVCDAHTPGSAVLIRAIEPRWG
                     MDRMADRRRTAKPMDLCSGPGRLCQALGISGSLNDLPINAPPFTLDFGDEAPLVVGPR
                     IGIRHGAETLWRFGKADSRFLSRPFP"
     gene            complement(747..2984)
                     /locus_tag="VF633_13225"
     CDS             complement(747..2984)
                     /locus_tag="VF633_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX8472065.1"
                     /translation="MARYPLQALTVAVLLVSTSLISPALAQTPPVAGVEAAWLTPPPR
                     PALDFKPDALPSRAAVMPALDYVAASQIADMSRRPIALSTGSNLTEISSNWVAATFYI
                     GAARLARVSEDPEILRFLTAAAEHYNYSLRGARSEKLMLNADEVTIGDLYEELYARRG
                     QQGTLMPLQQRLDFMVPHLARSADTPALVWWWSDALYMAPPVLARMTTITGDPKYLRA
                     MDTEWRRTAGRLWNPEERLFLRDERFKDRPSGNGELIYWSRGNGWVMGGLARVLEEMP
                     ADFANRPFYVDIFQKMAGRIMELQQADGLWRSSMLDPQAFPEAETSGSGFFVYALAWG
                     INHGLLDREITLPHVTRGWAALNRHVTAEGLIGAAQKTGDQPVSTAETDVGLYATGAY
                     LLAGIEVMDLNGPAQSLPEAPPVRDSAAVIAATTPTPPAPVTVVGEEEKQRRAAEMTA
                     TQALAYDPATLNRPSTVEPLAPPSAEDQRPRAVARFAPDRLDDLLWENDRVAHRIYGP
                     ALQSREPPSGSGVDVWAKRVRWPFMDRQLRFPNYHTDRGEGLDYYDVGRGRGAGGLGV
                     WYDNKLWTSRNFQTYEILKTGGDEAKFKVNYAPWPVDVSRSVSETRTFSLPLGSNFTR
                     MTSTIQSDQTTPLTIAIGISKRQNDAGRGYVTKDAANGRLMFWEPNDSAHGSLGIAIL
                     VDPAMVQGFAEDGDNYMILLRVTPGQPFVYYMGSAWDHGLDFASREAWETFAASQTPD
                     FDPGR"
     gene            3133..4524
                     /locus_tag="VF633_13230"
     CDS             3133..4524
                     /locus_tag="VF633_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEX8472066.1"
                     /translation="MHRRTLIQLGLVGIAGAVAPHAMAQVTGRLFDAADYGAVGDGRA
                     LDTRSIQAAIDAAESAGGGVVRLRPGVYLSGSLFVKSGVTLEIGKGATLRGSRELSAY
                     PEIRTRVAGVEMTWPAALVNVYRQKNAKITGEGVIDGDGKVFWDSYWAMRRDYDPREL
                     RWAADYDCRRPRLIHVYESDDIEVSGLELTRSGFWTVHVCYSRQVTVSDLVIRNNIGG
                     RGPSTDGIDIDSCDGVRVERCDLSVNDDAICIKAGRDWDGLRVARPCRNVVIKDCIVR
                     DALAGMTFGSETSGGFQNIEVTGLRIEYPVPLGVFFKSGHTRGGVISDIRMRDIHLQD
                     VRTLFRVNLNWYPEYSYAQIPAGVTDIPDYWTALATPVPREQGIPRIHNVRVSDVVAV
                     GGKVGFEAAAYPEAPLEDFTFERLNWDVQDAGTIANAKDWRFVDCNIDTLDGKGPKVT
                     DSTGVTGLRSRVA"
     gene            complement(4614..7430)
                     /locus_tag="VF633_13235"
     CDS             complement(4614..7430)
                     /locus_tag="VF633_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8472067.1"
                     /translation="MNRPRDARTATVMGVSASALLFALGSATTVAAQTAPVETAPAQT
                     DSVQTTPDDDAAQVGDIVVTGFRSSLQRALNIKKSEAGAVDVILAEDMADFPDQNLAE
                     AIQRLPGVTIDRVNGQGTTISVRGLGSDFTRTRINGLEAQAALGGNRNRSFDFSMFAS
                     ELFNSIKVRKTQSAEIEEGSLGATVDLQTGRPLDFSDSGFQSALSAQASYNDLSEKTI
                     PRLAGLLSWSNPDRTFGALLSVAYSERSPVLGSFNSTRWQSGNANPTPLPGQTRPAAF
                     PYAFGQNFGGCIPCTTTEQRDTLLNAYYPRIPRYTLGQTFEDRLGMTGALQWRPSDRT
                     EVNLDLLYSRFNSETESPNLEAWSFSRNAINNVIVRDYAIDASRNALSYGVFDNMIVA
                     AENGFTRNESTFAQVSLNARHDFTDRLHGTLKVGVNRSEARTPISEAYRFEAPGINGY
                     TFDFRDDSRQPMINYGFDTTSGSRFALTNATKSTGGGNFENDVFAGALSYDWTDQISF
                     KGGGEHRTYSFETFGLARAKTVPTGAEALAGVDKWGRVVSIDGYVDAPAGSTLSFIVP
                     DLAKLAASLSFYNDPLVPNRSEREVEETDNGVYLQSDFNTMVGGWTVRGNVGIRYAQT
                     DITAKGWQTVSTGAPPVISYNYVTTENSYDDVLPSFNLAFEPRDNVVLRLGAAKVMSR
                     PTLADLTPGGSVSPTTLTVSYGNPLLDPFRATNYDASIEWYFQKEGLLALAVFHKDIS
                     SFITSETVKLPYGQLGLPLELLQGVAGLNANTEFLVTRRLNGQGGTLDGVELQYQQPF
                     TFLPGAWSNFGFIGNVTWVTSDVNYGATAGNNRLTGQSKNTANATLYYETGGFQARVS
                     AAYRSQYLVAFPGANGNSEEGVNAATNIDASMSYDITPSMTVSLEGINLTDEYTDRYV
                     DATDRVSDYRHTGREVAVGLRWKY"
     gene            complement(7609..9060)
                     /locus_tag="VF633_13240"
     CDS             complement(7609..9060)
                     /locus_tag="VF633_13240"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEX8472068.1"
                     /translation="MTTSRRTALKALLAAPAAGVFAGAVQAQTAPIAAPVDATATGAP
                     VFPAPNTAPPIHQVGDSPPPFPTWSGPVPTDWVDPKTGHRIVRLSGDEGGNKLYFYRS
                     MFTPEGDVLVFSSSAGIIKVELSNGFRRSVLVAETNADLLLTGRKTRTAYYTVSEPGD
                     APPTDRPRILFAVDIDTGVKRRLARIEQGQFGSVNADETLIVGTVAFGDTPLQPGAAD
                     PRNRRLDQAEYSAVGPDGKPLNFAKSKGVRMLQRWAARVPMELFVVDLKTGERRVIHS
                     ATDWLNHPQFSPTDPDRIIFSHEGPWHRVDRMWTIKVDGTDLRKLHQRTMNFEIFGHE
                     WFAPDGKSVMYDLQTPRGQVFWVASVDLETGKRFYRRVEQNSWSVHFNSAPDGSFFAG
                     DGGDADMVAHAPDGKWLVLLHPEPVIDGDVESDKDDLISVEYLRTERLVDMGLHDYRL
                     EPNVTFTPDGKWVLFVSNMHGRNHVYAAQVAKA"
     gene            complement(9087..>9330)
                     /locus_tag="VF633_13245"
     CDS             complement(9087..>9330)
                     /locus_tag="VF633_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472069.1"
                     /translation="LDEHANDLVPVSGGAGGVAMSVNPDGAGYRRAAETYFPSNGSLL
                     MAVGLMAAGWDGSTGHAPGFPNEGWTVRVEGLTPAP"
BASE COUNT         1637 a         2804 c         3160 g         1729 t
ORIGIN      
        1 gttgagcgac ggtcgtgatg gaccttgcag gggatagtgg ccggcctttg ctcaggggaa
       61 gggtcgactg aggaagcgtg aatcggcttt gccaaatcgc cacagcgttt ctgccccatg
      121 gcggatgccg atgcgggggc caaccaccaa cggagcctcg tctccgaaat ccagggtgaa
      181 tggcggcgcg tttatcggga gatcgttcag cgatccggag attccgagcg cctggcacag
      241 ccgtcccggg ccggagcaca ggtccattgg ctttgctgtt ctgcggcggt ccgccatgcg
      301 gtccataccc cagcgaggtt cgatcgccct gatcagcacg gcgctgccgg gagtatgggc
      361 gtcgcaaacg acattcaggc accagtggag gccataaatc cggtagacgt acgtccgccc
      421 gacggggccg aacattgcag cattgcgtgg cgttcgaccc ctgaaactgt gagatgcagg
      481 atccagagca gcgtatgctt cggtttccac gataatcccg ccaacgccgt ctacggtgag
      541 gcttgcacca atcaatgaag cagcgacttt cggtgcttcc gcccctagcc ggtcggcaat
      601 gtcagacgac atgtcagcgt ccttgatcag gggcggcaga gaacgcgtgt cagcatcatg
      661 ggtagtcgtc attaatgctg gcgtgggacc caagcaaacc gtccgctttc gcccggacct
      721 gcggatgcag gtctgggcga tgtcgtttaa cgcccgggat cgaaatccgg tgtctggctg
      781 gcggcgaatg tttcccaagc ctcacggctg gcgaagtcga ggccgtggtc ccaggccgat
      841 cccatataat aaacgaacgg ctgacccggc gtgacacgca acaggatcat gtagttgtct
      901 ccgtcctccg cgaagccctg gaccatggcc ggatcgacca ggatggcgat gccgaggctg
      961 ccgtgggccg agtcgtttgg ctcccagaac atcaggcggc cgttggcggc gtccttggtg
     1021 acatagcccc gaccggcatc gttctggcgc ttggaaatac cgatggcgat ggtgaggggc
     1081 gtggtctggt ccgactggat cgtcgaggtc atccgggtga agttggaccc tagcggcagg
     1141 gagaaggtcc gggtttccga gacgctgcgg ctcacatcca caggccacgg cgcgtagttg
     1201 accttgaact tcgcctcgtc gccaccggtc ttgaggattt cgtaggtctg gaaattgcgc
     1261 gaggtccaaa gcttgttgtc ataccagaca cccagaccgc cagcgcctcg accgcgtccg
     1321 acgtcgtagt agtccagacc ctcgccgcga tcggtgtggt agttcgggaa ccgcagctgg
     1381 cggtccatga agggccagcg cacgcgtttg gcccagacgt cgacgcccga tcccgacggc
     1441 ggttcgcgcg attgtagcgc agggccgtag atgcggtggg cgacgcggtc gttttcccag
     1501 agcaggtcgt ccagacgatc cggcgcgaag cgggcgacgg cgcgcgggcg ctggtcctct
     1561 gccgaaggcg gggcgagggg ctcgacggtc gatggccggt tcaaggtcgc ggggtcatag
     1621 gccagagcct gtgtcgcagt catctcggcg gcgcggcgtt gtttttcttc ctcgccgacg
     1681 acggtaaccg gggcaggcgg ggtcggcgtg gtcgcggcta tcacggcggc gctgtcgcga
     1741 acgggcggcg cttcgggcag ggactgagcc gggccgttca ggtccatgac ctcgatcccg
     1801 gccagcagat atgccccggt ggcatagagt ccgacgtccg tctcggcggt cgagacaggc
     1861 tggtcgccgg tcttttgcgc ggcgccgatc aggccctcgg cagtgacatg ccggttcaac
     1921 gccgcccagc cgcgcgtgac gtggggcagg gtgatctcgc ggtccagcag gccgtggttg
     1981 atcccccacg ccagggcgta gacgaagaag cccgatcccg acgtctctgc ttcggggaac
     2041 gcctgcgggt cgagcatgct ggagcgccat agaccgtcgg cctgttgaag ttccatgatc
     2101 cgtccggcca tcttctggaa gatgtccaca tagaaggggc gattggcgaa gtcggcgggc
     2161 atctcctcaa ggacgcgggc gaggccgccc atgacccagc cgttgccgcg cgaccagtag
     2221 atcagttcgc cgttgccgga tgggcgatcc ttgaagcgtt catcgcgcag gaacaggcgc
     2281 tcctcggggt tccagagacg cccggcggtg cgacgccact cggtgtccat ggcgcgcagg
     2341 tatttgggat caccggtgat cgtggtcatc cgcgccagca ccggcggcgc catatagagg
     2401 gcgtccgacc accaccagac gagcgctggc gtgtccgcgc tgcgtgccag atgggggacc
     2461 atgaaatcca gccgttgctg cagcggcatc agcgtgccct gctgtcctcg tcgggcgtag
     2521 agttcttcgt agaggtcgcc aatcgttacc tcgtcggcat tcagcatcag cttctcggac
     2581 cgggcgccgc ggaggctgta gttgtagtgc tcagccgctg cggtcaggaa gcgcaggatt
     2641 tccgggtctt cggagacgcg agccagacgc gcagcgccga tgtaaaaggt ggccgcgacc
     2701 cagttggatg atatctcggt cagattgctg ccggtagaca gcgcgatggg ccggcggctc
     2761 atgtcggcga tctgcgacgc tgcaacgtag tcgagcgcgg gcatgacggc ggcgcgtgag
     2821 gggagggcat ccggcttgaa gtccagcgcc ggacgcggcg gtggcgtcag ccaggcggct
     2881 tcgacccctg cgacgggggg cgtctgcgcc aacgcgggtg atatgagcga cgtcgacacc
     2941 agaaggacgg cgaccgtcag ggcttggagg ggatatcggg ccatgcgcgt tcctctgtca
     3001 cggtgacaac aagccagtcc cgtgatttcc attatgtgta tttcagtacc ataaaatgga
     3061 tattgaggtg ggccaagcct cctgttgcga ataggctcgg cggaacaatc ataaccaacc
     3121 ggaggacgca ccatgcaccg tcgcaccctg atccagcttg gcctcgtcgg cattgccggg
     3181 gccgtcgcgc ctcacgccat ggcgcaggtc acgggccgat tgttcgacgc tgcggactac
     3241 ggggccgtgg gggatggccg ggcactcgac acccggtcca tccaggccgc catcgacgcc
     3301 gccgaaagcg ccgggggcgg agtggttcgg cttcggcccg gcgtttatct aagcggatcg
     3361 ctgttcgtga aatccggcgt gacgctggag atcggcaagg gcgcgacctt gcggggttcg
     3421 cgcgaactct cggcctatcc cgagatccga acccgcgtcg ccggggtcga gatgacctgg
     3481 cctgcggctc tggtgaacgt ctatcgccag aagaatgcga agatcactgg cgagggtgtc
     3541 atcgatggcg acggcaaggt gttttgggac agctattggg cgatgcgccg cgactatgac
     3601 ccgcgcgagc tccgctgggc cgcagattat gactgccgcc gtccccggtt gatccacgtc
     3661 tatgagtccg acgacatcga ggtctccggt ttggagctga cccgatccgg cttctggacc
     3721 gtccacgtct gttattcccg gcaggtcacg gtctctgatc tggtcattcg caacaatatc
     3781 ggcgggcgcg ggccttcgac ggacggcatc gacatcgact cctgcgatgg ggtccgggtc
     3841 gaacgctgcg acctctcggt caacgacgac gccatctgca tcaaggcggg ccgcgactgg
     3901 gacggtcttc gggtcgcgcg gccgtgccgc aacgtcgtga tcaaggactg tatcgtccgc
     3961 gatgcgctgg ccggcatgac attcggcagc gaaacctcgg gcggtttcca gaacatcgag
     4021 gtgacgggcc tgcggatcga atatccggtc ccgttaggcg tcttcttcaa gtcgggacat
     4081 acccgtggcg gggtcatcag cgacatccgc atgcgcgaca tccacctgca ggatgtccgc
     4141 accctcttcc gcgtgaacct caactggtac ccggaataca gctacgccca gatcccggct
     4201 ggcgtcaccg acattcccga ctactggaca gcactggcga ccccggtgcc gcgcgagcag
     4261 ggcatcccac gcatccacaa tgtccgggtt tccgacgtcg tcgcggtcgg tggcaaggtc
     4321 ggtttcgagg ccgccgccta tcccgaagcg ccgctggagg atttcacctt cgagcgcctg
     4381 aactgggacg tgcaggatgc ggggacgatc gcaaacgcca aggattggcg ctttgtcgac
     4441 tgcaacatcg atacgctcga cggcaaggga ccgaaggtga cggactccac cggggtgacg
     4501 ggtcttcgct cccgcgtcgc ctgacgcaag ccgctgatcg ccagacgaaa aaatggcccg
     4561 gagcggtgac gctccgggcc tgagttatgc gctgaatccc ttgggaggaa gattcagtat
     4621 ttccagcgca atcctactgc gacctcgcgg ccggtatgac ggtagtcgga gacccggtcg
     4681 gtcgcgtcga catagcggtc agtgtactcg tcggtcaggt tgatgccttc gagcgagacc
     4741 gtcatgcttg gcgtgatgtc gtaggacatg gacgcgtcga tgttggtggc ggcgttcaca
     4801 ccctcttctg agttgccgtt ggcccccggg aaggccacca gatactggct gcggtacgcc
     4861 gccgaaaccc gggcctggaa gccgccggtc tcgtagtaaa gggtggcgtt ggccgtgttc
     4921 ttggactggc ccgtcaggcg gttattgccc gccgtagccc catagttcac gtccgaggtg
     4981 acccacgtca cgttgccgat gaagccgaag ttcgaccacg cgcccggcag gaaggtgaac
     5041 ggctgctggt actgcagttc gacgccgtcc agcgtcccgc cttggccgtt caggcggcgg
     5101 gtgaccagga actcggtgtt cgcgttaagt ccggccacgc cttgcagcag ctcaagcggc
     5161 agacccaact ggccgtaggg aagtttgacg gtttccgatg tgatgaagct gctgatgtcc
     5221 ttgtggaaca cagcaagggc gagcaatccc tctttctgga aataccattc gatcgacgcg
     5281 tcatagtttg tggcgcggaa cggatcgagc agcggattac cgtaggagac ggtgagagtc
     5341 gtcggcgaca cgctgccgcc gggcgtcagg tcggccaagg tcggccgcga catgaccttt
     5401 gcagcgccaa gacgcaggac gacgttatcg cgtggttcaa aggccagatt gaacgacggc
     5461 agaacgtcgt cgtagctgtt ttcggtcgtg acgtagttgt aggatataac gggcggcgca
     5521 cccgtagaga cggtctgcca gcccttggcg gtgatgtcgg tctgcgcata gcggatgccg
     5581 acgttgcccc gcacggtcca accgcccacc atggtgttga agtcggactg gagatagacg
     5641 ccgttgtccg tctcttcgac ttcgcgctcg ctgcgattgg ggacgagcgg atcgttgtag
     5701 aagctgagcg aggcggcgag tttggcgagg tctgggacga tgaaggacag ggtcgaacct
     5761 gcgggcgcat cgacatagcc gtcgatgctg accacccggc cccacttgtc gacgccggcc
     5821 aaggcctcgg cgccggtcgg tacggtcttg gcgcgggcca ggccgaaggt ttcgaagctg
     5881 taggtccgat gctcgccgcc gcccttgaag ctgatctggt cagtccagtc gtaggacagc
     5941 gccccggcga agacatcgtt ctcgaaattg ccgcctccgg tgctcttcgt cgcattcgtc
     6001 agcgcgaaac gcgaacccga ggtcgtgtcg aagccgtagt tgatcatcgg ctggcggctg
     6061 tcgtcacgga agtcgaaggt gtagccattg atgccgggcg cctcgaaccg ataggcctcg
     6121 ctgatcggcg tacgcgcttc cgagcggtta accccgacct tcagtgttcc gtgcaggcga
     6181 tccgtgaaat catgacgggc gttgagcgag acttgagcga aggtgctttc gttgcgggtg
     6241 aatccgttct ccgcagccac gatcatgttg tcgaacacgc cgtaagacag ggcgttgcgc
     6301 gaagcatcga tcgcgtaatc gcgcacgatc acattgttga tggcgttacg gctgaacgac
     6361 caggcttcga gattggggct ctccgtctcg gagttgaagc gcgagtagag caggtcgagg
     6421 ttgacctcgg tccgatcgct cgggcgccac tgcagagcgc cggtcatccc caggcggtcc
     6481 tcgaacgtct ggccgagagt atagcggggg atgcgcgggt agtacgcgtt caggagcgtg
     6541 tcacgctgct cggtggtggt gcagggaatg cagccaccaa aattctgtcc aaaggcatat
     6601 ggaaatgcag ccgggcgggt ctgaccaggc aagggcgtgg gattggcgtt gcccgactgc
     6661 cagcgcgtcg agttgaagct gccgagcacc ggtgagcgct ccgaataggc taccgacaac
     6721 agcgcgccga aggtcctgtc cggattggac cagctcaaca ggccggccaa ccgtggaatg
     6781 gtcttctcgg acaggtcatt atacgaggct tgagccgata aggcggattg gaagccgcta
     6841 tcactgaaat cgagcggtcg gccggtctgc agatcgacgg tcgcgcccag cgagccttcc
     6901 tcgatctcgg cggactgggt cttgcgcacc ttgatgctgt tgaacagctc cgatgcaaac
     6961 atcgaaaagt cgaagctgcg attgcgattg ccgcccagcg ccgcttgagc ctcaagaccg
     7021 ttgatgcggg tgcgggtgaa gtccgagccc aggccccgga ccgaaatggt cgtgccttgg
     7081 ccattgacgc ggtcgatggt cacgccgggc aggcgctgga tggcttcggc gaggttctgg
     7141 tcggggaaat cagccatgtc ttccgccagg atgacatcga cggctccggc ctcgctcttc
     7201 ttgatgttca gagcgcgctg aaggctggag cggaagccgg tcacgacgat atcgcctacc
     7261 tgcgcagcat cgtcgtcggg agtggtctgg acagaatcgg tctgcgccgg agcggtctca
     7321 acaggcgcgg tctgggcggc gacggtcgtg gccgagccga gcgcgaagag cagagcggag
     7381 gccgaaacgc ccatgacggt cgcggttctg gcgtcgcgtg gtcggttcat cgtcttctct
     7441 ccccgcaccg acgcgcgtgg caccggtgtc atttgttggt atggtatcgc ggtagttgga
     7501 cgcgagttcg ttccacttat tgcaacagct tctcgctttt tgcaacaccg agactttcgc
     7561 agcgacaaga tggcttgatc ttggtgaggc gaggacgtgg cttggggctc aggccttggc
     7621 gacctgcgcc gcatagacgt gatttcggcc atgcatattg gagacgaaca gcacccattt
     7681 cccgtcgggt gtgaaggtca cgttgggctc gagccggtag tcgtgcagac ccatatccac
     7741 cagccgctcc gtgcgcagat actcgaccga gatcaggtcg tccttgtcgc tctccacatc
     7801 gccgtcgatc acgggttcgg ggtgcaacaa gaccagccat ttgccgtccg gcgcatgggc
     7861 gaccatgtcc gcatcgccgc cgtctccggc gaagaacgat ccatcaggcg cgctgttgaa
     7921 gtggacggac cagctgttct gctcgacgcg gcggtaaaac cgcttgccgg tttccagatc
     7981 gaccgacgcg acccagaaca cctgaccgcg cggggtctgc aggtcataca tcaccgactt
     8041 gccgtcgggc gcgaaccact catggccgaa gatttcgaaa ttcatggtgc gctggtgaag
     8101 cttgcgcagg tccgtcccgt cgaccttgat ggtccacatc cggtcaaccc ggtgccaggg
     8161 tccctcgtgc gagaagatga tccggtcggg gtcagtgggg gagaactgcg ggtggttcag
     8221 ccagtcggtg gcggaatgga tgacgcgccg ctcgccggtc ttcagatcaa cgacgaacag
     8281 ctccatcggc acgcgcgcgg cccaacgctg caacatgcgc acgcccttgg acttggcgaa
     8341 attgagcggc ttgccgtcgg gtcccacagc cgaatactcg gcctgatcga ggcggcgatt
     8401 gcgcgggtcg gccgcccccg gctgtagcgg cgtgtcccca aaggcgaccg taccgacgat
     8461 cagggtttcg tccgcattga cggatccgaa ctgcccttgt tcgatgcgcg caagacggcg
     8521 cttcacaccc gtgtcgatgt ccaccgcaaa tagaattcgg ggccgatcgg tcggcggagc
     8581 gtctcccggc tccgagacgg tgtagtaggc ggtgcgggtc ttgcgaccgg tcagcagcaa
     8641 gtcggcattg gtctcggcca ccagcacaga acggcggaac ccgttgctga gttcgacctt
     8701 gatgatccct gccgacgacg agaacaccag cacgtcgccc tcgggcgtaa acatgctgcg
     8761 atagaaatag agcttgttgc cgccctcgtc gccggacagc cgcacgatgc ggtggccagt
     8821 cttgggatcg acccagtcgg tgggaaccgg gccgctccag gtcggaaacg ggggagggct
     8881 atcgccgacc tgatggatcg gcggggcggt gttcggggct ggaaacaccg gggcgcccgt
     8941 cgctgtcgca tcgacgggcg ctgcgatcgg tgcggtctgg gcctgaacgg cgcccgcaaa
     9001 cacgcctgcg gcgggagcgg ccagcagggc cttcagggcg gtacggcgag aggtcgtcat
     9061 gggggcgttc ctcgtgtttt gatgtgtcag ggcgcgggcg tcaggccttc gacacggacg
     9121 gtccagcctt cgttggggaa gccgggcgca tgaccggtcg agccgtccca ccccgccgcc
     9181 atcaggccca cggccatcag cagagatccg ttggacggga agtaggtttc ggccgcgcga
     9241 cgatagccgg cgccatccgg gttgaccgac atcgccacgc cgccggcgcc gccggacacc
     9301 ggcacgagat cgttggcgtg ttcgtccaga
//
LOCUS       DASVTM010000480         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1002005,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000480 DASVTM010000000
VERSION     DASVTM010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002005"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VF633_13250"
     CDS             complement(<1..951)
                     /locus_tag="VF633_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEX8472070.1"
                     /translation="MSRVLVIGAGGVGSVAVHKMAMNADIFSHITLASRTKSKCDAIA
                     ASVKERTGVTIDTAAIDADDVAATTALINAVKPALVVNLALPYQDLNIMDACLAAGVN
                     YMDTANYEPRDEAKFEYKWQWAYQDRFKDAGLMALLGSGFDPGVTSVFTTYTKKHLLD
                     RIDTLDILDCNGGDTGLPFATNFNPEINLREVTAPSRHWENGQWIEGPALTHKQVFDF
                     DQVGQKNMYLMYHEELESLAKFYPEISRIRFWMTFGDSYLKHLEVLENIGMTSIEPMM
                     FQGREIIPIEFLKALLPEPSSLGPLTKGKTNIGTIATGLKD"
     gene            complement(1129..>1921)
                     /locus_tag="VF633_13255"
     CDS             complement(1129..>1921)
                     /locus_tag="VF633_13255"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8472071.1"
                     /translation="TARRLVLERGSPAGHDRAIMHVSQPILEVFRPAVPNGSAMIVAP
                     GGGYVRLAIDKEGAGAARRLNEAGITAFVLNYRLPGDGWAAGYDAPLQDMQRAVRLVR
                     ARAADWALDAQRIGVMGFSAGGHLAAASLTRYDDSVYAAVDATDQTSARPDFVCLGYA
                     LMSVGSGANQYPGPDAEARQPLNERVRSGMAPTFLVHAADDRTVPVGHSLAMFAALKA
                     ADVPAEMHIYQEGGHGFGFTLPADRPASHWPDAFEAWARRSGFLG"
BASE COUNT          328 a          593 c          655 g          345 t
ORIGIN      
        1 gtccttcagg ccggtggcga tggtgccgat gttggtcttg cccttggtca ggggacccag
       61 cgacgatggc tcgggcagca gggccttaag gaactcgatc gggatgatct cgcgaccctg
      121 gaacatcatc ggctcgatcg acgtcatgcc gatgttctcg agcacttcga ggtgcttcag
      181 atagctgtcg ccgaacgtca tccagaagcg gatgcggctg atctccgggt agaatttcgc
      241 cagcgattcc agctcctcat ggtacatgag gtacatgttc ttctggccga cctgatcgaa
      301 gtcgaaaacc tgtttgtggg tcagggccgg accctcgatc cactggccgt tttcccagtg
      361 acgcgacggc gcagtgactt cgcgcaggtt gatctcggga ttgaagttgg tggcgaacgg
      421 caggccggta tcgccgccgt tgcagtccag aatgtcgagc gtgtcgatcc ggtcgagcag
      481 gtgtttcttg gtgtaggtgg tgaacacaga tgtcacgccg ggatcgaagc ccgaacccag
      541 cagagccatc agacctgcgt ccttgaagcg gtcctgatag gcccactgcc atttgtattc
      601 gaacttggcc tcgtcgcgcg gctcgtagtt ggctgtgtcc atatagttca caccggccgc
      661 cagacaggcg tccatgatgt tcagatcctg atacggcagg gccagattga ccacgagggc
      721 gggcttcacg gcgttgatca gggcggtggt tgcggccacg tcgtccgcgt cgatcgccgc
      781 cgtgtcgatg gtcacgccgg tgcgctcctt caccgaggcg gcgatggcgt cgcatttgga
      841 cttcgtgcgc gacgccaggg tgatatgcga gaagatgtcg gcgttcatcg ccatcttgtg
      901 gaccgcgacc gaacccacgc cgcccgcacc gattaccagc accctgctca ttgaacgcac
      961 tcctgaagac ttgaaaactc tggcggccga gattgagttc gaggccggtc ctgcgaaggg
     1021 cggggcttct agcacgggcg cgcgcgcttc gctaaccacc gaggtcgcat attaaccgcc
     1081 gaccgatgac atcggtgtgt cggagatggc tttgaagaaa gtgcggcgct acccgaggaa
     1141 gcccgaccgc cgcgcccaag cctcgaacgc gtccggccaa tgcgatgcgg gtcggtcagc
     1201 cgggagggtg aagccgaagc cgtggccacc ctcctgatag atgtgcatct cggctgggac
     1261 atctgcggcc ttgagtgcgg cgaacatcgc cagactatgg ccgacgggga cagtgcggtc
     1321 gtcggcggcg tggacgagga acgtcggcgc catgccggac cggacccgct cgttcaacgg
     1381 ttgacgggcc tcggcgtcgg ggccggggta ctggttcgcg cccgatccga cgctcatgag
     1441 ggcgtagccg aggcagacga agtcgggccg cgccgaggtc tggtccgtgg cgtcgaccgc
     1501 cgcatagacg ctgtcgtcgt accgggtcag cgaggccgcc gcgaggtgcc cgccggccga
     1561 gaagcccatg acgccgatcc gttgggcgtc caacgcccag tcggcggcgc gggcgcggac
     1621 cagacgcacg gcccgctgca tgtcttgcaa gggcgcatca tagcctgcgg cccagccgtc
     1681 gccgggcagg cgatagttga gcacgaaggc ggtgatcccc gcttcgttca ggcgacgcgc
     1741 ggctccggcg ccttccttgt cgatggccag ccggacatag ccgccgccgg gcgcgacgat
     1801 catggccgac ccgttcggta cggcgggccg gaagacctca aggatcggct gtgagacgtg
     1861 catgatcgcc cggtcatggc cggcaggcga cccacgctcc agcaccagac ggcgggcggt
     1921 a
//
LOCUS       DASVTM010000481         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1056870,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000481 DASVTM010000000
VERSION     DASVTM010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056870"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF633_13260"
     CDS             complement(<1..400)
                     /locus_tag="VF633_13260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8472072.1"
                     /translation="MIRTATAGAVLAILLGSAALAQTAPEAAPPPPPPAPASAPPSEW
                     RTVAPENLMVINTTKGQVLVELAPEIAPLHVERMRLLARNGFFDNLTWHRVIDLFMAQ
                     TGDPLGTGDGQSAYPDIKAEFTFRRGPELPF"
     gene            complement(423..1340)
                     /locus_tag="VF633_13265"
     CDS             complement(423..1340)
                     /locus_tag="VF633_13265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8472073.1"
                     /translation="MNSRPLSILAAAVAGVLITAGQAGAQILSPTPPQGPAAAATPLP
                     PADAPDWRIVSPDNLLVIDTSKGRILVELDSRVAPQSVERIRTLTARGFYDGLKFHRV
                     IRGFMAQTGDPLGTGAGGSDLPDVPGEFTFRRGRNADFAIVPNAGAGVHAILGGMPLL
                     TQPDAQMFVTADMKVDASGMFCPGIAGMARSGDPNSANSQFFLMSGANANLNGAYTTF
                     GRIVSGLGVVAALNAGPDSSDGAVGPNADVMTKVQLASAIPEGQRPTAKVLNPTSPAF
                     VQRYEATRAKVGNAFSICDVETPSIVTGG"
     gene            complement(1337..>1801)
                     /gene="coaD"
                     /locus_tag="VF633_13270"
     CDS             complement(1337..>1801)
                     /gene="coaD"
                     /locus_tag="VF633_13270"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX8472074.1"
                     /translation="DPVTNGHADIIRRAVKLVDKLVIGVAINAGKGPMFSLEERVDML
                     VEATAPLTQYAVIEVRPFETLLMHFAREVGAQMIVRGLRAVADFEYEFQMTAMNQRLN
                     QDIETVFLMADPRHQAIASRLVKEIARLDGNIESFVSPAVAAHVRDRVKKHV"
BASE COUNT          319 a          554 c          621 g          307 t
ORIGIN      
        1 cgaacggcag ctccggtcca cggcggaagg tgaactccgc cttgatgtcg ggataggcgc
       61 tctggccgtc gcccgtaccg agcgggtctc ccgtctgggc catgaacagg tcgatgaccc
      121 tgtgccaagt cagattgtca aagaagccgt tacgggccag cagccgcatc cgctccacgt
      181 gtaagggcgc gatctcgggc gcgagctcga ccagcacctg acccttggtc gtgttgatga
      241 ccatcaggtt ctcgggcgca accgtgcgcc actcggaagg tggagccgaa gctggggctg
      301 gcggaggcgg cggaggggca gcttcgggcg cggtctgcgc caaggctgcg gagccgagca
      361 gaatggccag aacggcccct gctgttgcgg ttctgatcat gtcgcgccct cccctgatgc
      421 cgtcagccgc cggtcacgat ggatggcgtt tccacgtcgc agatgctgaa cgcattcccc
      481 actttcgccc gggtcgcctc gtaacgctgg acgaaggccg ggctggtggg attcagaacc
      541 ttcgccgtcg ggcgctggcc ttcgggaatg gctgatgcga gctgcacctt ggtcattacg
      601 tcggcgttcg gaccgaccgc gccgtcggac gaatccggtc cggcgttcag ggcggcgaca
      661 acaccaaggc ccgacacgat acggccgaag gtggtgtagg cgccgttcag gttggcgttg
      721 gccccgctca tcaggaaaaa ctggctgttg gcgctgtttg ggtcacccga ccgggccatg
      781 ccggcgatgc ccgggcagaa cataccgctc gcatcgacct tcatgtcagc ggtcacgaac
      841 atctgggcgt caggctgggt aagcagaggc atgccgccga ggatggcgtg aacgccggcc
      901 cctgcattgg gcacgatggc gaaatcggcg ttgcggccac ggcggaacgt gaactccccc
      961 ggaacgtccg gcaggtcgct gccaccggcg ccggtgccga ggggatcgcc cgtctgggcc
     1021 atgaagccgc gaatcacgcg gtggaacttg aggccgtcat aaaagccgcg tgcagtcagg
     1081 gtgcggatac gctcgacgct ctgcggcgcg acacgggaat ccagctcgac gaggatccga
     1141 cctttggaag tgtcgatgac cagaaggttg tcgggcgaaa cgatgcgcca gtccggcgca
     1201 tctgcaggag gcaaaggcgt cgcggccgct gctggtccct gcggcggtgt gggggacaaa
     1261 atctgagcgc cggcctgacc agcggtgatc agcacaccag caacggcggc ggcgagaatg
     1321 gagagcggac gcgagttcat acgtgtttct tgaccctatc ccgcacgtgc gcagcaacgg
     1381 cggggctgac gaagctttcg atattcccgt ccagacgggc gatctccttg accagacggg
     1441 aggcgatcgc ctgatggcgc ggatcggcca tcaggaacac agtttcgatg tcctgattga
     1501 ggcgctggtt catggccgtc atctggaatt cgtactcgaa gtcggccacg gctcgcaggc
     1561 cccggacgat catctgcgcg ccgacctcgc gtgcaaagtg catgagcagg gtctcaaacg
     1621 gacgaacctc gatgacggca tattgcgtca agggcgcggt ggcctcgacg agcatgtcga
     1681 cgcgttcctc gagcgagaac atcggcccct tgccggcgtt gatcgcgacg ccgatgacca
     1741 gcttgtcgac gagcttcacc gcccggcgaa tgatgtcggc gtggccgttg gtcaccgggt
     1801 c
//
LOCUS       DASVTM010000482         6297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_844796,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000482 DASVTM010000000
VERSION     DASVTM010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844796"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VF633_13275"
     CDS             complement(<1..427)
                     /locus_tag="VF633_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8472075.1"
                     /translation="MQKRALGKSGLETAPLVFGGNVFGWTIDRNQSFDLLDAFVGAGF
                     NAIDTADVYSRWVPGNTGGESESIIGDWLKARGRRDDVLILTKVGSPMGQNGDDDRKD
                     LSKHWIARAVEASLKRLKTDYIDLYQSHWMDQVTPTEETL"
     gene            complement(509..970)
                     /locus_tag="VF633_13280"
     CDS             complement(509..970)
                     /locus_tag="VF633_13280"
                     /inference="COORDINATES: protein motif:HMM:NF009972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472076.1"
                     /translation="MQGCHAFRRWASIKQAERMTARILIIEDEALVAMELRFVLEDLG
                     YEVVGSAADARTARNLVKETEVDLALVDIHLQDGPTGVELGRDLAQTDGVTVLYMTAN
                     PGMVRGGVSGAIGVLSKPADERAVQKAVDYALRRRNGDPVEYAPPELQLFA"
     gene            complement(988..2937)
                     /locus_tag="VF633_13285"
     CDS             complement(988..2937)
                     /locus_tag="VF633_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8472077.1"
                     /translation="MLSSSSSIPPVSDRWHERDRVAALRAYGVLYTAPETEFDDIVAL
                     AARICDAPTALISLVDSDVQWFKARVGLEVCETARDVSFCDHAMREHDVMVVADATLD
                     PRFAENALVTGDPGIRFYAGAALVTSEGFPFGSLCVIDYRPRPDGLTDLQTQTLTVLA
                     RQVVTQLELRRALKQREQSEETALRAMTASDYVGAWDWDIVNDRVVTDVKLARMYGVD
                     PAVGVAGAPIESFSGAVHADDMKRVREDIRRALKGDGDFFSEYRIALEGLPLRWILVR
                     GQVYSGPDGAPERVSGLAVDVTERKQMDADLAGAALALSESEAKFRAIADSMPQMVWS
                     TQPNGFHDYYNARWYEFTGVPAGSTDGEGWNAIFHPEDQPRAWEVWRRSLETGELYEI
                     EYRLRRHDGVYRWTLGRAAPILNEAGEIVRWFGTCTDIDDLKRMEQGKELLSQELSHR
                     IKNIFAVVSALIALSAREYPEAKGFAAAVRNRIGALARAHEFVRPHSEVSRPTVGGMT
                     LHAFLEALFRPYADDAGAPRVLIDGDDALFDDQAATSVALLFHELATNAAKYGALSEA
                     GGRVVLNTRLNGDRFILTWTERGGPAMAGPPTRAGFGSSLATVSVEGQLAGKLERIWE
                     AGGLIVTADLPATALSRRMAAEKPR"
     gene            complement(3047..4252)
                     /locus_tag="VF633_13290"
     CDS             complement(3047..4252)
                     /locus_tag="VF633_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8472078.1"
                     /translation="MIAIDWIRAKRQGRRVAAAAPAMLAALGVAMAASSTPRPEIDRT
                     AEAVARATGGDLTDRGLAAMTARLTPSQLALAMRNDPNERQAALYGLTPGWESLSLAG
                     KPTLELGANGLNAQRMNAAMLPAVGALRPALPFVFKAASEADRQRAVRCLTQGIYYEA
                     ALESTEGQEAVAQVILNRVRDPNYPSTICGVVFQGAERNTGCQFSFTCDGALSQAPVG
                     WAWERARTVALRSLAGHVASKVGTATHYHADYVHPWWSPTLAKITQIGAHIFYRWKGV
                     YGETAAFNQRYASREPVIDEARFSRPRLMLASNAPGTPAVLDATGKPLTTGMKTVQID
                     GQTRVVGVVSLGGRRAPTTEEIAAINSRMNAFENPQMPAPIAGPAPRVVTAPGATAMT
                     VEEVGKPVS"
     gene            complement(4366..4845)
                     /locus_tag="VF633_13295"
     CDS             complement(4366..4845)
                     /locus_tag="VF633_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEX8472079.1"
                     /translation="MTAAIDTGLSKAERTDVVGQLTKVLADSYALYLKTHGYHWNVRG
                     PEFFSVHSLLEQQYREMWEALDTIAERIRALGEFAPQGYATFANLTSIGDGDPEKDSN
                     AMLAELIKDHGTVIATMRAALKVSDDEGDDVSVDLMTQRLAAHEKAAWMLRSTLGSK"
     gene            5046..>6297
                     /locus_tag="VF633_13300"
     CDS             5046..>6297
                     /locus_tag="VF633_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8472080.1"
                     /translation="MDATAFRRRVLTIDALLARVARTRARSAGANVLIGLAYAAGFLL
                     LRFATSAFYKDFTGLMILLPAVVLAALMAGRIAGAAAVFACLVGGWLLAGSGGPGGLQ
                     TPPGQMATINFLLAGLFVTLVAASLRKTVNSLDETVAALKMSDVRAGQTELELGAIID
                     QASAGIARVGLDGVIETTNARFAEILATTKDRLVGSSTADITHPDDVAPTRALLASAL
                     VGGAGQIEKRYYRGDGSVVWALTSLQRLLGTDGLPKGFIAVIVDISEAKQTEAALRES
                     EERFRLMADTAPSPVWLTNAEGEVEFVNAALAAFYGASPSEIFGHVWRQSIHPQDVSI
                     VIAAQSEARSDHKPYGFEARFRRHDAHWRWMRVSVNPRFDGQGRFQGYVGMSFDVTET
                     REAIEALDHQAQRQRFLLSLTDTLR"
BASE COUNT         1096 a         2023 c         1985 g         1193 t
ORIGIN      
        1 gcagggtttc ttccgtcgga gtgacctggt ccatccagtg cgactgatag aggtcaatgt
       61 agtcggtctt cagccttttc aggctggcct cgaccgcgcg tgcgatccag tgcttcgaga
      121 gatccttgcg gtcgtcgtcc ccgttctggc ccatcggcga gccgaccttt gtcaggatga
      181 ggacatcgtc gcgccggccc cgcgccttga gccagtcgcc aatgatgctc tcgctctcgc
      241 cgccggtgtt tccgggaacc caacgcgagt agacgtccgc cgtgtcgatg gcattgaagc
      301 ccgcgccgac aaaggcgtcg agcagatcga acgactggtt cctgtcgatc gtccagccga
      361 agacgttgcc gccgaagacc agcggtgcgg tctcaagacc gctcttgccg agggcgcgtt
      421 tctgcatggg aagctccaat tcgggggagc tttggactta tgccccacga acgggtccgc
      481 aaccacaagg gatgcgatta gcgtgttttc aggcaaaaag ctgaagttcc ggcggggcat
      541 attcgacggg atcaccgttg cgacggcgaa gggcgtaatc gacggccttc tgcaccgccc
      601 gctcgtccgc cggcttggac agaacgccga tggcgccgga gacgccgccg cgaaccatcc
      661 cggggttggc ggtcatgtag agaacggtga cgccatctgt ttgagcgagg tcgcgaccca
      721 gttccacgcc ggtggggccg tcctgcaggt ggatatccac caatgccaga tcgacttccg
      781 tttccttaac cagattccgg gcggtacgcg catcggcggc gcttccaacg acctcgtagc
      841 cgaggtcttc caggacgaag cggagctcca tggctacgag ggcttcgtcc tcaatgatca
      901 ggatacgcgc agtcattctt tcagcttgct tgatcgacgc ccaacgcctg aacgcatgac
      961 atccttgcag gtttcacacg cgttacgtca gcgcggcttt tcggccgcca tacgtcgaga
     1021 aagcgcggtc gcggggaggt ccgcagtaac gatcaaaccg ccggcctccc agatccgttc
     1081 caattttccg gccagttgtc cctcgacgga aacggtcgcc agtgacgatc cgaaccccgc
     1141 gcgcgtcggc gggcccgcca ttgcgggtcc gccgcgctcg gtccatgtga gtatgaagcg
     1201 atcaccattc agccgggtat tcagcaccac ccgaccgccg gcctccgaca gggcgccgta
     1261 tttggcggcg ttagtggcca gttcgtggaa cagcagggcg acggaggtcg cggcctgatc
     1321 atcgaacagg gcgtcatcac cgtcgatcag gacgcggggc gcaccggcat catccgcata
     1381 aggcctgaac agggcttcca ggaaggcgtg cagggtcatg ccgcccactg tcgggcgcga
     1441 cacctcgctg tgcggcctta cgaactcgtg cgccctcgcc agcgcgccga tgcggttgcg
     1501 aacagcggcg gcgaatccct tggcttcggg atattcccgc gccgacaggg caatcagcgc
     1561 cgagaccacg gcgaagatat tcttgatgcg gtggctcagc tcctggctca ggagctcctt
     1621 gccctgttcc attcgcttga ggtcgtcgat gtccgtgcag gtgccgaacc accgaacgat
     1681 ctcgccagct tcgttcagga tcggtgcggc gcgtcctagc gtccagcgat agacgccgtc
     1741 gtggcgacgc aggcgatatt cgatctcgta aagctcgccc gtctccagcg agcgccgcca
     1801 gacttcccag gcacgcggct ggtcttccgg atgaaaaatg gcgttccagc cttcaccgtc
     1861 ggtcgagcct gccgggacac ccgtaaactc ataccagcgg gcgttatagt agtcgtggaa
     1921 cccgttcggc tgggtcgacc agaccatctg cggcattgaa tcggcgatgg cgcggaattt
     1981 cgcctcgctt tctgacaggg ctaaggccgc ccctgccaga tctgcgtcca tctgcttgcg
     2041 ttcggtgaca tctaccgcaa gccctgacac ccgttccggc gcgccatccg ggccggaata
     2101 gacctgcccc cggacgagga tccagcgcaa cggcaagcct tcgagcgcta tccggtattc
     2161 gctgaagaaa tcgccgtcgc ccttgagggc ccggcggata tcttcgcgca cgcgcttcat
     2221 gtcgtccgcg tggacagcgc cggaaaaact ttcgatcggg gcccctgcga cgcccactgc
     2281 gggatcgacg ccgtacatgc gcgcgagctt cacgtcggta accacccggt cattgacgat
     2341 atcccaatcc cacgcgccga catagtccga agctgtcatc gcgcggaggg cggtctcttc
     2401 gctctgttcg cgctgcttca gggcccggcg cagctccagc tgggttacga cctggcgcgc
     2461 cagcaccgta agggtctggg tttgcagatc agtcaggcca tcgggccggg gcctgtagtc
     2521 gatcacgcaa agcgatccga acgggaaccc ctcggatgtc accagtgccg ccccggcgta
     2581 gaagcgaatg cctgggtcgc cggtcaccag cgcattctcg gcgaagcgcg gatccagtgt
     2641 ggcgtcagcc accaccatga cgtcatgttc gcgcatcgca tggtcacaga aggagacgtc
     2701 acgggccgtt tcgcagacct caagcccgac cctggccttg aaccactgca cgtcgctgtc
     2761 gaccaggctg atcagggccg tcggcgcgtc gcagatgcgc gcggccaagg cgacgatgtc
     2821 gtcgaactcc gtctcgggcg cggtgtagag cacgccgtaa gcccgcagcg cggcgacacg
     2881 gtcacgctcg tgccagcggt cggaaacggg agggatcgaa gaagaggagg acaacattca
     2941 gccttgtgca ggcgcttggc gccgtctgct aaatgcagag ccggttgcac cgttgcaagc
     3001 cgaccttgtc tggttcacgt cacacggtac tgacaaccga ccacagtcag ctaacgggtt
     3061 tgccgacttc ctcgaccgtc atcgccgtcg ccccgggcgc cgtcacgacg cggggcgcag
     3121 gccctgcgat gggagcgggc atctggggat tttcgaaggc gttcattcgc gagttgatcg
     3181 cggcgatctc ttcggtcgtc ggcgcgcggc ggccaccgag gctgacaacg ccgaccacgc
     3241 gggtctggcc gtcgatctgc accgtcttca ttccagtggt cagaggcttg cctgtagcgt
     3301 ccagaaccgc cggcgtcccg ggtgcattcg aggccagcat caagcgcgga cgcgagaagc
     3361 gcgcctcatc gatgaccggc tcacggctgg cgtagcgctg gttgaaagcg gcggtttcgc
     3421 catagacgcc cttccagcga tagaagatat gcgcaccgat ctgggtgatc ttggccagcg
     3481 tcggcgacca ccacgggtga acatagtcgg cgtgataatg ggtcgccgtg ccgaccttgc
     3541 tcgcgacgtg accggccagc gaccgcagcg ccaccgtccg ggctcgctcc caggcccaac
     3601 ccaccggcgc ctgtgagagc gctccgtcgc aggtgaagct gaactggcag ccggtgttgc
     3661 gctcggcccc ctggaagacc acgccgcaaa tggtgcttgg ataattgggg tcgcgcacgc
     3721 ggttcaggat cacctgagcg acggcttcct ggccctcggt gctttccagc gccgcttcgt
     3781 aatagatgcc ttgcgtcagg caacgtacgg cgcgttggcg gtccgcctcg ctggcggcct
     3841 tgaacacgaa cggcaaggcg ggacggagcg cgcccaccgc aggcagcatc gctgcattca
     3901 tccgctgggc gttcagccca ttcgcgccca gctcaagcgt tggctttccg gcgaggctca
     3961 ggctttccca acctggcgtg aggccataaa gcgcagcctg tctctcgttg gggtcgtttc
     4021 gcatcgccag agccagttgc gaaggcgtta gtcgtgcggt cattgccgca agacccctgt
     4081 cggtgaggtc gccccccgtc gcacgcgcga cggcctcggc ggtgcggtcg atctcgggac
     4141 gcggagtcga actcgcggcc atggcgacgc ccagcgcggc cagcatggcg ggcgcagctg
     4201 cagcgactct ccggccctgc cttttcgctc tgatccagtc gatggcgatc attctatttc
     4261 cgtacggcga cgtccaggaa cccatggcgc tgcgtcagat ggctgggcct tctgacccac
     4321 cttctgggct gttttcaggc cacagattgc cgttcgttca gactcctact tcgaccccag
     4381 cgtagaacga agcatccagg cggccttttc gtgagccgcc agacgctggg tcatcaagtc
     4441 gacggaaacg tcgtcgcctt catcgtcgga taccttcagg gcagcgcgca tggtggctat
     4501 gaccgtcccg tgatccttga tcaactcggc cagcatggcg ttcgagtctt tctccggatc
     4561 accatcgccg atcgaggtca ggttggcgaa cgtcgcgtag ccctgcggcg caaactcgcc
     4621 cagcgcgcgg atacgttcag cgatggtgtc cagcgcctcc cacatctcgc ggtactgctg
     4681 ctcgagaagg ctatgcaccg agaaaaattc cgggccgcgt acgttccagt gatagccgtg
     4741 cgtcttgagg tagagagcgt agctgtccgc gagaactttg gtcagttgcc cgacgacatc
     4801 ggtccgctca gccttgctga ggcctgtatc aatggctgcg gtcatgtttg ctccggagtt
     4861 tcaggcatct gtatcgagga cgtgcagtta ggcattgccg agcccaaatc caaccccggc
     4921 aaagcgtcca gcctccagag tacgggcggg cgccatagcc tttcggcagt ttgggaacgc
     4981 agcgctctac caagtgttgc agcgacgcgg tgagggccgc actatatccc atggccgggg
     5041 gaggaatgga cgcaaccgcg ttccgcagaa gagttctgac gatagacgcc ctcctggcgc
     5101 gggtggcgag gacgcgcgct cgttccgctg gcgcaaatgt tctgatcgga cttgcctatg
     5161 ctgcgggatt cctgctgctg cgcttcgcga cgtcggcctt ctacaaggat ttcacgggcc
     5221 tgatgatcct tttgccggcc gtggttctgg cggcacttat ggcggggcgt attgcgggag
     5281 ccgctgcagt tttcgcctgc ttggtcgggg gctggctact ggccggatcg ggagggcctg
     5341 gaggtctcca gacgccgccg ggccagatgg caactataaa tttcctgctc gctggcctgt
     5401 tcgtaactct ggtggcggcc tcgctgcgca agaccgtcaa ctccctggat gagacggttg
     5461 cagcgctgaa gatgtcggat gttcgggcgg gccagacgga gctggagctg ggcgcgatca
     5521 tcgaccaggc ctcggcaggc atcgcccgtg taggcctcga cggcgtaatc gagaccacca
     5581 atgcccggtt cgccgaaatc ctcgccacta cgaaggacag actcgtcgga tcatccacgg
     5641 ccgacatcac ccatcccgac gacgtcgcgc ccacgcgggc cttgctggcg tcggccctgg
     5701 tcggcggtgc aggtcaaatc gaaaagcggt attaccgggg agatggctcc gtcgtctggg
     5761 ctctgacaag tcttcaacgt ctcttgggga ctgacggtct gcccaagggc ttcatcgctg
     5821 taatcgtcga catttcggaa gccaaacaaa ccgaggcagc cctgcgcgaa agcgaggagc
     5881 gcttccgctt gatggccgac accgcgccct cgccggtctg gctgacaaat gctgaaggcg
     5941 aggtggagtt cgtcaacgca gccctggcgg cgttctacgg cgcttcgccc tctgaaatct
     6001 tcggccacgt ctggcgacaa tcgatacatc cgcaagacgt ttccatcgtg atcgccgccc
     6061 agagtgaagc gcggtcggat cacaaaccct acggcttcga ggcccgtttc cggcgacatg
     6121 acgcgcactg gcgctggatg agggtgtcag ttaatccacg cttcgacggg caaggcaggt
     6181 tccagggcta tgttggaatg tctttcgacg tgaccgaaac acgcgaggcc atcgaggctc
     6241 tcgatcatca ggcccagcgc cagcgttttc tgttgtcgct caccgacacc ctgcgtg
//
LOCUS       DASVTM010000483         6258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_951375,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000483 DASVTM010000000
VERSION     DASVTM010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951375"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VF633_13305"
     CDS             <1..937
                     /locus_tag="VF633_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX8472081.1"
                     /translation="NSDAFDTYLELFNAAGESVDQDDDGGEGGVPIEDGEGGSTNARL
                     ISTIKDAGTYTLQVRAIGDTQAGAYTLKLAEAAPAPAATALPFGETVQGEITADGARD
                     DEGRGYRAYTFSGTAGNRVQVVMRSGDFDTFIQVGRPGDAFEALGSDDDGLGEGTDSR
                     LNYILPETGEYEIRVSPLSGDEKGLFSLELTDKGPQPEPGSILVGATARGTLDESDGI
                     ADDGAFFDAYAITVAEGDKLNVTLVSNDFDSYLDLGKMTDGAWVSTVSDDDSLSDTHA
                     KVEWTVEDAGTYMIRARSFTQGETGAYTLAVERQP"
     gene            1003..1590
                     /locus_tag="VF633_13310"
     CDS             1003..1590
                     /locus_tag="VF633_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX8472082.1"
                     /translation="MPDLTPDLFGEARAPADRLFLAIFPDAEAVARIAEAAEDLRREH
                     GLKRLVHPLDHLHITLFHLGDFAGVPRDLVRNVSAAAEAMKVAPFEVTFDHATSFPGR
                     PGSRPFVLKGGLPDETRLAELNTCRQALGLALAAQGVRTTAAFTPHITLLYDDPVIAP
                     CPIDPISWTVNELVLVRSHIGKNVYEHIGRWPLQG"
     gene            1760..2116
                     /locus_tag="VF633_13315"
     CDS             1760..2116
                     /locus_tag="VF633_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEX8472083.1"
                     /translation="MTARMYAIPACDTVKKARDWLAANGVEYEFHDYKKLGASLPILE
                     QAIAEHGWEVVLNRAGTTFRKLPDDARANLDARKAVDLMLAQPSMIKRPIVVVEGLTL
                     VGFKPEAYAATFLVRP"
     gene            complement(2296..2751)
                     /locus_tag="VF633_13320"
     CDS             complement(2296..2751)
                     /locus_tag="VF633_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8472084.1"
                     /translation="MTELSRTFLGRLSEQLSDLIEQQSAEVFKRAGLIIPVKSSSLLG
                     AIATRSPVSVSDLVRALNRSHQLIQQKIPKLVTLGLVTRRPNPDDQRVILIEITDKGR
                     DQLALLDKLAPEFDRAYAGMEEDAGPVFDAIDRAIHALKSRPLLDRIAG"
     gene            complement(2767..3987)
                     /locus_tag="VF633_13325"
     CDS             complement(2767..3987)
                     /locus_tag="VF633_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8472085.1"
                     /translation="MDRDAQRDVVSHLEAALRANYVFPDRIPAMSAELERRVQSGPIE
                     SKAFAVSLAQGLVKASDDLHFSVAFDPVEVAADVRAKASGETTSQEQRDSERAANFGF
                     RDARRLDGGLAYVRFDFFADPQYAQETAVGAMRFAEGAKGLIIDLRYNNGGVLEMAQF
                     LMSYLYPAGKEQEFFDYNYTDHGVRVERSQWNLAAVPGQRSGDIPVVVLTGSTSFSAA
                     EWMAFSLQKLGRATIIGQQTAGGAHPITRVPIDDNFMLQAPIGQIRDPVTALDFEGVG
                     VTPDVVVPASEALLAAQRFLLQPRADAGDAEAKWALIPVEFAISGRPPSTTDLDDAVG
                     AYDGRTLVRTATGLAYHWHGRFVLALDPIGKDLFAVQGTDDYRFRLVRENGAVIGLER
                     VFKSGETASYRRLD"
     gene            4223..5203
                     /locus_tag="VF633_13330"
     CDS             4223..5203
                     /locus_tag="VF633_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8472086.1"
                     /translation="MTSIAGEPNALTLPNLPQSNISRPFWRRPTAGTLAILGVVICAL
                     IWGTTWFAITFQLGTVDPTASIVWRFGLASVILFAVCLVMRSPIRLTRAQHLAAAGQG
                     VFAFALSYTFVYKAEGLISSGVVAVAFASMALMNLILFRLVEKQTAARTVWIGAGLGA
                     LGVAVLSGGELFSARLGPDVGLGIALALAAAAVSTLANWFAWRGQRAGSQVIPATAWA
                     MAYGAGLVVLFGLATGVHWSISLTPGYLGSLLYLAVLGSVVAFVIYFTVARSHGYTLA
                     SYIGALVPLIALTVSVLFEGARFGVSAFAGVALVIAGQIMLTRTPKAPAA"
     gene            complement(5216..5401)
                     /locus_tag="VF633_13335"
     CDS             complement(5216..5401)
                     /locus_tag="VF633_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEX8472087.1"
                     /translation="MVEVINLNHARKARAKDEARATAAANRVAHGRTRGQREVAEAER
                     DRATRLLDGAKLKPEGD"
     gene            5513..5752
                     /locus_tag="VF633_13340"
     CDS             5513..5752
                     /locus_tag="VF633_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX8472088.1"
                     /translation="MVDATLIKEHLEVVGSDGGHVGRVDHVLGDQIELAKLDLAGGFK
                     HHMIPVSWVAHVDEHVHLNITQEEAKSRWTEKPKD"
     gene            complement(5812..>6258)
                     /locus_tag="VF633_13345"
     CDS             complement(5812..>6258)
                     /locus_tag="VF633_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HEX8472089.1"
                     /translation="NPAPHRARSALYVVVNGQVGRNTGVTPGRIDGIPARTYDSMSKA
                     SLRLSLAATAAFASRNGMTVEMSAIPDNVTVSSLKFDTATMQALFEMGRTRSASGEAW
                     ASVTPSGRPGPLVAIQNAPAEQIPEALATPPTAAEAPANTEDAPKP"
BASE COUNT         1082 a         2042 c         1957 g         1177 t
ORIGIN      
        1 gaactccgac gccttcgaca cctatctgga actgttcaac gccgccggcg aaagcgtgga
       61 tcaggacgac gacggcggag aaggcggcgt tcccatcgag gacggtgagg gcggcagcac
      121 caatgcccgc ttgatttcta cgatcaagga cgccggaacc tatacgctgc aagtccgggc
      181 catcggagac acgcaggccg gcgcctatac gctgaagctg gctgaggctg cgccggcgcc
      241 cgccgcgacg gccctgccgt ttggcgaaac cgttcagggc gagatcaccg cggacggggc
      301 ccgcgatgac gagggtcgcg gctatcgcgc ctatacgttc agcggcacgg cggggaaccg
      361 ggtgcaggtg gtgatgcgtt ccggcgactt cgacaccttc atccaggtcg gtcgtccggg
      421 cgacgcgttc gaggccctgg gcagtgatga cgacggtctg ggcgagggta ccgactcgcg
      481 tctgaactac atcctgccgg aaaccgggga gtacgagatc cgcgtgtcgc cgctgagtgg
      541 tgacgagaag ggcctgtttt ccctcgaact gactgacaag gggccgcagc ctgagccggg
      601 aagcattctc gtgggcgcca ccgcgcgcgg cacgctggac gagagcgacg gcatcgccga
      661 cgacggcgcc ttcttcgacg cctacgccat caccgtggct gagggcgaca agctgaacgt
      721 caccctggtg tcgaacgact tcgactccta cctcgacctc ggcaagatga cagatggcgc
      781 atgggtcagc accgtcagcg acgacgacag cctgtcggat acccacgcca aggtcgagtg
      841 gacggtcgag gacgcgggga cctacatgat ccgcgcccgc agcttcaccc aaggcgagac
      901 gggcgcctat acgctggcgg tcgaacgcca gccgtaaggc acaagtccgg cctcgaacgt
      961 ttcgaggtcg gacccctgtt tgcgcccgaa tccggaggcc tcatgcccga cctgacccca
     1021 gacctgttcg gcgaggcgcg ggctcctgct gaccgattgt tcctggccat ttttcccgat
     1081 gccgaagctg tcgcgaggat tgcagaagcg gcagaggacc tgcgtcggga gcatgggctg
     1141 aaaaggttgg tccatccgct cgaccatctg catatcaccc tgtttcacct cggcgatttc
     1201 gcgggcgtgc cgcgcgattt ggttcggaac gtgtcggctg cggcggaggc gatgaaagtc
     1261 gcgccgttcg aggtgacctt cgatcacgcg accagcttcc cgggtcggcc gggtagtcga
     1321 cccttcgtgc tgaaaggcgg gctccccgac gaaacacggt tggccgaact gaacacctgc
     1381 cgtcaggctc tcggactggc tcttgcagcc cagggcgtga ggaccacggc cgccttcact
     1441 ccccacatca ccctgcttta cgatgaccct gtgatcgcac cctgtccgat cgaccccatc
     1501 tcgtggacgg tcaacgagct ggtgctggtg cgaagtcata tcggtaagaa tgtctacgag
     1561 cacatcggtc ggtggccgct gcaaggctag acgtcgcgac gaacattcac ggcaacagat
     1621 cctggtgacc acgacggtct gcggaacagc cggatccgag cgagtccccc agtcgtggat
     1681 ctcgccggtc tggtctggtc tggtctggta cgctgttgtc tagcgtacta tggctccgga
     1741 gccgaaggga gagcccggta tgactgcgcg aatgtacgct atccctgcct gcgataccgt
     1801 caagaaggcg agggactggc tcgctgcgaa cggcgtcgag tacgagtttc acgattacaa
     1861 aaagctggga gccagcctgc cgatcctgga gcaggctatc gcggaacacg gatgggaagt
     1921 cgtgttgaac agggctggca cgacattcag gaagttgccg gatgacgcca gggccaacct
     1981 cgatgcccgc aaggccgtcg acttgatgtt ggcgcagccg tcgatgatca agcggcccat
     2041 cgtcgtcgtc gaaggtctta cgctcgtcgg gttcaagccg gaggcctacg cagcgacctt
     2101 ccttgtcagg ccatagcccg gcgtcttctt ttcccgccga ccgaggggat cacgccgggg
     2161 ccaggctgtg cgtggtccgg ggtcaaaccg ccttcgaagg cttcctcgat gacgatacgg
     2221 aacgtgtgct ggcgacccgg cccacccgtg gaacgctcga tctttccgcc cggactgcgg
     2281 gcaagggcgt gcgcttcagc cggcgatgcg atccagcaga gggcgcgatt tgagagcgtg
     2341 gatggcccgg tcgatagcgt cgaacacggg gccggcgtct tcctccatcc ccgcataggc
     2401 gcgatcgaac tcgggggcca gcttgtcgag cagagccagt tgatcgcgcc ccttgtctgt
     2461 aatctcgatc aggatgacgc gctgatcgtc gggattgggc cgacgggtga ccagccccag
     2521 cgtcaccagc ttcgggatct tctgctgaat gagttggtgt gacctgttca gcgcgcgcac
     2581 gaggtcggac acggagaccg gactccgtgt cgcgatcgcg cccagcaatg acgaggactt
     2641 gaccggaatg atcagcccgg cgcgcttgaa cacctcggct gattgttgct cgatcagatc
     2701 gctcaactgc tcgctgaggc gacccaggaa tgtgcggctc aactctgtca ttcgcccaac
     2761 agtccctcag tccagacggc ggtagctggc cgtttcgcca gatttgaaca cccgctcaag
     2821 cccgatcacg gcgccgtttt cgcgcaccag acggaaccgg taatcgtccg tgccttgaac
     2881 cgcaaacaga tctttcccga tcggatcgag agcgagaacg aagcggccat gccagtggta
     2941 ggccagaccg gtggctgtgc gaaccagcgt tcgaccgtcg taggccccga cggcgtcatc
     3001 cagatcggtt gtgctggggg gccggccgga gatcgcgaac tcgaccggta tcagagccca
     3061 ctttgcctcc gcgtcgcctg cgtccgcacg gggttgcaga aggaatcttt gcgccgccaa
     3121 aagagcctcc gaggcgggca cgacgacgtc aggggtcaca ccgacgccct cgaaatccag
     3181 cgcggtcacc ggatcccgaa tctgaccgat cggcgcctgc agcatgaagt tgtcatctat
     3241 cggcacacga gtgatgggat gcgcgccacc ggcggtttgt tggccgatga tggtcgcacg
     3301 gccaagcttt tgcagtgaaa acgccatcca ttcggcggcg gaaaaggagg tcgatccagt
     3361 cagcacaacg accggaatgt ctcctgaacg ctgcccgggc accgcagcca ggttccattg
     3421 actccgctcc acacgaaccc cgtggtccgt atagttgtaa tcgaagaatt cctgttcctt
     3481 gcccgcaggg tacaggtaac tcatcagaaa ctgcgccatc tccagcacgc cgccattgtt
     3541 gtagcgcaag tcgatgatga gaccctttgc gccttctgcg aaccgcatgg ccccgacggc
     3601 cgtctcctgg gcgtattgcg gatcggcgaa gaagtcgaac cgcacatagg cgaggccgcc
     3661 atccagccga cgagcgtcgc ggaagccgaa attcgccgcc cgctcgctgt cgcgctgttc
     3721 ctggctcgtg gtctcgccgc tggctttcgc ccgaacatcg gcggcgacct ctacgggatc
     3781 gaatgcgacc gagaaatgca ggtcgtcact cgccttgacc agaccctgag cgagactgac
     3841 ggcgaatgcc ttgctctcga tcgggccgga ttgaacccgt ctttccaact cggcggacat
     3901 ggcggggata cggtcaggga agacgtagtt cgctctcagc gccgcctcca gatgagacac
     3961 gacgtcccgc tgtgcgtcac ggtccaaagt ctgcgcacca gtcgttgctg gagccagtgt
     4021 cacggccgcc aacagcgaaa caaaaacccg tagaaacatc cgcaatccaa tcatgaaaga
     4081 tattgcgcaa tatgttgtat gttgtcgctc gtcaatctct acatcccggc gcggtttgcc
     4141 ggtttgccgc gcgttcataa gccaagcttg tgtgtccagc gcgacattcc acttgccgac
     4201 cgaccctcgt ccgtccatcg cgttgacctc aatcgccggt gagccgaacg ccttgaccct
     4261 gcccaacctt ccgcaatcaa acatttcccg cccgttctgg cgccgaccca ccgccggaac
     4321 ccttgcgatt ctgggcgtcg tcatctgcgc cctgatctgg ggcacgacct ggttcgccat
     4381 cacgtttcag ctcgggaccg tggatccgac tgcctcgatc gtgtggcgct tcgggctggc
     4441 gtcggtgatc ctgtttgcgg tctgccttgt gatgcgcagt cccatccgcc tgacgcgcgc
     4501 ccaacatctg gccgcggcgg ggcagggggt gttcgccttc gcgctcagct atacgttcgt
     4561 ctacaaggcg gaggggctga tttcctcggg cgtcgtcgcc gtagccttcg cctccatggc
     4621 cctgatgaac ctgatcctgt tccgtctggt cgagaagcag accgccgctc gaacggtctg
     4681 gatcggcgca ggtctcggag cgctcggtgt tgcagttctt tcgggcggag agctgttcag
     4741 cgcgcgtctg gggccagatg tcggtctcgg catcgcactg gctctcgcag ccgccgccgt
     4801 ctcgaccctg gcgaactggt tcgcctggcg tggccagcgt gcgggatcgc aggtcatccc
     4861 ggccaccgcc tgggccatgg cctacggcgc cggcctggtg gttctgttcg gcctcgccac
     4921 aggggttcac tggtcgatca gcctgacgcc cggttatctc ggttcgctgc tgtatctggc
     4981 tgtgctcggc tcagtcgtgg cgttcgtgat ctacttcacc gtcgcccgga gtcacggata
     5041 caccctggcc agttatatcg gcgcgctggt cccgctcatc gctttgacgg tgtcggtcct
     5101 gttcgagggc gcacggttcg gcgtctcggc cttcgccggc gtcgcactcg tcattgccgg
     5161 ccagatcatg ctgacccgaa caccgaaggc gcccgccgcc tgaacgacaa tcggctcagt
     5221 cgccttcagg cttcagcttc gccccgtcca gaagccgcgt agcccgatct cgctcggctt
     5281 cggcgacctc ccgctgacct cgggttctgc cgtgcgcgac gcggttggcg gcggctgtag
     5341 ctctcgcctc atccttggcg cgggctttgc gcgcgtggtt cagattgatc acctcgacca
     5401 cttgttcctc ctgtgacgct tcatatttcg ccggcccgtc tggcgttttg ctgaaaccgc
     5461 accgcttcgg tcgcgttgtc atcggcaacg ccacaccaaa cggagagacc ccatggtaga
     5521 cgccacactc atcaaggaac atctggaagt cgtcggttcg gacggcggcc acgttggccg
     5581 ggtcgatcac gttctgggcg accagatcga actcgccaag ctcgacctcg cgggtggctt
     5641 caagcatcac atgatcccgg tcagctgggt cgctcacgtg gatgaacacg tccacctgaa
     5701 catcacccag gaagaagcca agtcccgctg gaccgaaaag cccaaggact aggcatccgc
     5761 ctcatccgat tgaaaggccc cgtcatcgac ggggcctttt gcctgtccgg ctcagggttt
     5821 cggagcgtct tcggtattgg ccggcgcctc agccgcagtt ggcggggtcg ccagcgcctc
     5881 cggtatctgc tcggccggcg cgttctggat cgccaccaac ggcccgggcc gcccggacgg
     5941 cgtgaccgag gcccacgcct ctccgctggc cgaccgcgtc cggcccatct cgaacaaggc
     6001 ctgcatggtg gccgtgtcga acttcaggct ggagacggtg acgttgtccg gaatggccga
     6061 catctcgacc gtcatgccgt tccgcgacgc gaacgccgcc gtcgccgcca gcgacagcct
     6121 cagcgacgcc ttgctcatac tgtcataggt ccgcgccgga atgccgtcga tccgccccgg
     6181 cgtgacgccg gtattgcgcc ccacctgtcc gttgacgacg acatagagcg cgcttctggc
     6241 ccggtgcggc gccgggtt
//
LOCUS       DASVTM010000484        18797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_896253,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000484 DASVTM010000000
VERSION     DASVTM010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896253"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..641)
                     /locus_tag="VF633_13350"
     CDS             complement(51..641)
                     /locus_tag="VF633_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX8472090.1"
                     /translation="MKSLVIGAAALASLLGASAASAQTAGPANVGPAIPGVCVFFNQQ
                     LLAQSTAGQSVQTRMGQLATEVQGELQPYATTIQTEAQALQQGASTIAPADLQSRRQA
                     LQQRAQEAQQLQQTREDELRYTLSEQRRLISVAVEPLLVAIYQEKGCGILLDRESVFI
                     MNPAMDVTADVIARLNTQLPTLSFNRLPVPVQPQGQ"
     gene            complement(679..2961)
                     /gene="bamA"
                     /locus_tag="VF633_13355"
     CDS             complement(679..2961)
                     /gene="bamA"
                     /locus_tag="VF633_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX8472091.1"
                     /translation="MAPAAAAAPRPAETGVVNRILVEGNQRIDQTTVLSYLPIQPGDT
                     VDAVLLDVAVRTLNRTQLFANVQIGLQPNGDLVVSIVENPIINQVTFEGNSALSEKKL
                     TDEVTLAPRGIYTRAKVQEDVGKIIELYRLSGRISATVAPKLVQLEQNRVDVVFEIDE
                     GPETGLQAITILGNTAFSDTDLRDVMITKESRWYRLFSSNDNYDPNRLDYDQQQLRKF
                     YTNRGYYDFRIVSAVAELVPNNSAFGLTMTLDEGDKYNFGKINVVTENSRLNADFLKL
                     LIPIREGQLYESDKIESSVDALTFAAGSAGYAFVDIKPSYKANAETNTVDVTFNISEG
                     QRVYIDRINVVGNTRTIDPVIRREMMLTEGDAFNRTLIERSRNNLRALGFFKDVTIEE
                     VRGSAPDRTVVNVQVEEQPTGELSVGAGFSSVDSFVVNLGITERNFRGRGQNVVARAE
                     WGSLRQQLDFRFTEPRFLGRDVGAGFDVFHSRYDFQDESSFDYRSTGAGLRLSFPING
                     YTRLSTRYFIKDDEIIVPTGYCSTGGSGSRALCDQVGASLNSSAGYTLAIDRRNDPVR
                     PTRGWTGTMRQDLAGLGGDVNYVKTEAEASVFYGITPAWVVSVNGSVGYVAGWAGDAV
                     RINDRFFRGGNTFRGFETAGMGPRDLTTNDALGGNFYAVGTVELTLPNFLPETYGIKT
                     SLFTDIGTLGVLDDRYTLTSAGLQDANIVDDLSLRAASGVSIHWKSPMGPIRFDLSKV
                     WSQEEYDRTESFRFSTSTQF"
     gene            complement(3201..4421)
                     /locus_tag="VF633_13360"
     CDS             complement(3201..4421)
                     /locus_tag="VF633_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX8472092.1"
                     /translation="MLSALGQILIYIVPFLLVLTFIVTIHELGHYLIARLFNVKMDRF
                     AIGFGRALLSRTDKRGVEWRVGWMPLGGYVKFSGDLDAASVPDQAGLAELRSRVIAEN
                     GPGAERDYFHFKPVWQRALITAGGPVANFLLAIVLFAVLFGVVGEQIVAPRVYAVLPG
                     SPAAAAGFQSRDLITAIDGRSVRDGQEVTSTVLLSANTPLTFTVDRGGRSVSLTATPL
                     RQVVDDKIAGRVRMSRIGLDLRPMIRSDYTFRRLNPLEALSKGVHQVGDTLSTTLKYL
                     GRILTGRESGDQLSGPIGMAKASGALATAAVGADRAPVAIAINLVFTMVQLAAILSIG
                     IGFINLMPIPVLDGGHLVFYAYEAVAQKPVAARVQEAGYRVGLALLAGLMLFATWNDL
                     QKLNLFKFLGGLVS"
     gene            complement(4443..5639)
                     /gene="dxr"
                     /locus_tag="VF633_13365"
     CDS             complement(4443..5639)
                     /gene="dxr"
                     /locus_tag="VF633_13365"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX8472093.1"
                     /translation="MIRRRVSVLGSTGSVGCSTLELMAQAEAAGTGSFDVEVLTGGGN
                     VARLAEQARRWRPKQVVIADPAGLGALRDALADLAIEVAGGDGAVIEAATRPADWIMA
                     SIVGAAGLRSTWAAAGTGATLALANKESLVCCGMSLIARVKASGGRLVPVDSEHSAIF
                     QVFPADAPERVAKLILTASGGPFRQTSLAEMARMTPEQAVAHPNWSMGAKISVDSATM
                     ANKGLETIEAAYLFGMAAERIDVVVHPESIIHSMVEYVDGSTLAQMGPPDMKTPIACA
                     LAYPDRLEWPAPKLDLAALGRLTFEAPDHTRFPALKLARDALMAGGSAPTVFNAANEV
                     AAYAFLARRLTFLNIAAVVAETLDKATSAGMNSGYDDACDAALAVDAEARRIAEGIVA
                     GLAIAA"
     gene            complement(5636..6454)
                     /locus_tag="VF633_13370"
     CDS             complement(5636..6454)
                     /locus_tag="VF633_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX8472094.1"
                     /translation="MALKVGDIGIRAASAFVLAPAAVLATWAGGVWFLALILAACALL
                     AVEWAMMSAPGAWRMMTCAVALGLFAAVVTTHVGQLSLALVLLVFGAGAAGVFAKSRG
                     QEALNAAYGVLYLGWPAVLLVWLRDNHSPVGLHWTVLVLAVAWSADILAYLVGSAVGG
                     PKLWPRFSPNKTWSGFIGGLVAGMIAGAGMAAWLEMGRLNIFWGGVLGLAAALATMGG
                     DLWESALKRRFGVKDAGNLIPGHGGLLDRVDGLMFAVVIVSAGRWLVLLVERAA"
     gene            complement(6454..7209)
                     /gene="uppS"
                     /locus_tag="VF633_13375"
     CDS             complement(6454..7209)
                     /gene="uppS"
                     /locus_tag="VF633_13375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX8472095.1"
                     /translation="MTAFTADLPGTSEGPKHVALIMDGNGRWAQARGLPRAMGHREGV
                     QALKRTVQAAPQFGIECLTVFGFSTENWRRPAEEVSDLMGLVRSYVASDLNRLEREGV
                     RVRVLGRRTGLPTDIAAIIERAEAQTAHNSRFLLQVAFNYGGRADIVDAAQRHVDRVL
                     AGEASGPLDEDMLGAGLSTAGGPPVDLIVRTSGEQRLSNFLLWEAAYAELVFQNVLWP
                     DYGAGPLGEAVDQYLNRDRRFGGIMAQPALATG"
     gene            complement(7247..7804)
                     /gene="frr"
                     /locus_tag="VF633_13380"
     CDS             complement(7247..7804)
                     /gene="frr"
                     /locus_tag="VF633_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX8472096.1"
                     /translation="MAKPDLKTYRERMDKAISSLKEEYSGLRTGRAHAGLLDPVQVQA
                     YGSNSPLNAVAAISVPEPRMISVSVWDKTMVGPVEKAIRAAGLGLNPIVDGQTLRIPV
                     PPLTEERRKDLAKLAGKYAEQQKISVRNVRRDANDDLKKAEKAGEISQDEQKKMEAEV
                     QKDTDSAIKLIDQTLKTKEVEIMQV"
     gene            complement(7829..8572)
                     /gene="pyrH"
                     /locus_tag="VF633_13385"
     CDS             complement(7829..8572)
                     /gene="pyrH"
                     /locus_tag="VF633_13385"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX8472097.1"
                     /translation="MSDATAGPKYKRVLLKVSGEVLMGEQGYGIDMHTVDGVAAAIKR
                     VAESGTEICLVIGGGNIFRGLSKAAADMERASADYMGMLATVMNALAMQNALEKIGVQ
                     TRVQSAIPMAAIAEPYIRRRAVRHMEKGRVVIFAAGTGNPYFTTDTAAALRAAEMNCD
                     ALLKGTSVDGVYSADPKKDKTATRYEHLTYQDVLTQNLKVMDAAAIALMRESRIPIVV
                     FSIREEDSLRKVLNGQGTFTVISDNDVAA"
     gene            complement(8684..9628)
                     /gene="tsf"
                     /locus_tag="VF633_13390"
     CDS             complement(8684..9628)
                     /gene="tsf"
                     /locus_tag="VF633_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX8472098.1"
                     /translation="MAEITAALVMELRAKSGVGMMDCKKALQETDGDVSAAIDWLRAK
                     GLSKAAKKADRVAAEGLVAVASREDGKGEIGAAIEFNSETDFVARNELFQNAAKAFAE
                     HGLEHHSVEALHGAELEAGKTIQDEVTSMIATIGENMQLRRAARLSVSEGVVASYVHN
                     AVAPGLGRIGVLVALEGEGDKTALRELGRKIAMHVAATAPLSLNTDDLDPAAIEKERS
                     VLTEKAKEEGKPENMIAKIVEGQINKFQKDVVLTKQPFVMDPDVTIEQLVANSAKELG
                     SSNLHLAGFVRLALGEGVEKVEGPDFASEVASMMAPQA"
     gene            complement(9720..10610)
                     /gene="rpsB"
                     /locus_tag="VF633_13395"
     CDS             complement(9720..10610)
                     /gene="rpsB"
                     /locus_tag="VF633_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX8472099.1"
                     /translation="MALPEFSMRTLLEAGAHFGHQTHRWNPKMERYIFGSRSNIHIID
                     LSQSMPLFHQALVAVRDVAAKGGRVLFVGTKRQASDPVAEAAKRCAQYYVNHRWLGGT
                     LTNWRTVSGSIARLRELEVIMERGGEGRVKKELVNLGREKDKLELSLGGIKDMGSIPD
                     IMFVIDTNKEAIAILEARKLNIPVIAILDTNSDPDGITYPIPGNDDAARAIQTYCDLI
                     ADAVLDGLAAGASNSGVDLGASENPVEPMLAEASAPVAADVAPAGTEAVAEEMLVASG
                     EAQEATTEANDAVETEQATA"
     gene            complement(10927..11538)
                     /locus_tag="VF633_13400"
     CDS             complement(10927..11538)
                     /locus_tag="VF633_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8472100.1"
                     /translation="MIRTFAKPALAVVAVAALMAACNQQQTDKTAGDAGAAVNATQDA
                     TAGAVGQASAATLGANTVGGFATSLAMSDMYELEGAKIAASKSSKADIKELAAMITKD
                     HTASSTKLKAAAPTEAPGVTLPTTLDERRQGMIDNLNAASAADFDKVYLTQQVAAHNE
                     ALTLLNGFSDNTEAPTLAGLAKEIIPKVTMHRDHAKTMLDAMN"
     gene            complement(11647..12114)
                     /locus_tag="VF633_13405"
     CDS             complement(11647..12114)
                     /locus_tag="VF633_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX8472101.1"
                     /translation="MFTSILAAAVMMLAAQTSTTETTDMHHARGTFDVTITPVQPSAG
                     TAADVPGRMTLSKVFHGDLSGTATGEMLATMAPGASGAYVAMERVNGTLEGRSGSFAL
                     VHRGLMDKGAQDLLITVVPGSGTDDLQGITGVFHLTIEGGEHRYDLEYSLPQP"
     gene            complement(12114..15611)
                     /gene="dnaE"
                     /locus_tag="VF633_13410"
     CDS             complement(12114..15611)
                     /gene="dnaE"
                     /locus_tag="VF633_13410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX8472102.1"
                     /translation="MSRFRPASEDQAITDTVSSTGFVHLRVRSAYSLLEGAMKSGKIP
                     KLAADSGMPAVGVADRANLFGALEFSEASKGAGVQPLVACAMPVMGIGGKAGERWAKV
                     PTVVLICQTEAGWRNLCALSSSAYLDAGDNEPGVPWDKIVAHSEGLILLSGGPDGPVD
                     PLYVQNKNAEGDAALAEMARVFGDRFYVELQRHGLPHEGQGEQGLVTWAYANDVPIVA
                     TNDVYYAKQAQAKSHDALLCIADGAFTGQEDRRRITDQHWFKSVEAMRTLFADLPEAC
                     DNTIDIARRCAFLVNTHAPILPRFDAGAGRTEPDELAYQAREGLKVRLKEVTPAMPEA
                     EYWTRLEWEVGIIQQMGFPGYFLIVADFIKWAKNHGIPVGPGRGSGAGSLVAWALTIT
                     DLDPLRFGLLFERFLNPERVSMPDFDIDFCQERREEVIDYVQARYGKDRVAQIITFGT
                     LQARAVLRDVGRVLQMPLGQVDRLCKMVPNNPAAPVTLAQAIDLEPRLKEARDSEASV
                     RTLLETALELEGLYRNASTHAAGIVIGDRPLVELVPLYQDPRSTIPASQFNMKWVEPA
                     GLVKFDFLGLKTLTTLDRAREYLSRRGAEPVWNQLPLDDKATYELMASGQTVGVFQLE
                     SQGMRDTLRKMRCGSIEEITALISLYRPGPMEMIDTYIDRKFGRAEVDYLHPSLKEVL
                     TETYGVIIYQEQVMKIAQVLAGYSLGEADLLRRAMGKKKKEEMDFQKARFIKGASEKS
                     VPEQQSDSIFELVAKFAGYGFNKSHAAAYALISYQTGWLKANHPVEFFAASMSLDLSN
                     TDKLAVFYQDARKFEVPVMAPDVNRSSADFDVAWSEEKGAVLYALGAIRNVGLEAMKH
                     VVEVRETGGRFADIFDFLERVDPRAVNKRALEGLAKAGAFDTIHPNRRQLLEQADVLM
                     AYCQSVAAERASSQVSLFGADQANAARPRLRSVDPWIGPEQLDHELAAVGFYLSGHPL
                     EDMIPALKRKRVTFVAEATALAESGHEAFMMAGVIRRRQERASARTGEKFAFVNFSDP
                     TGEFECLFPPEQLRKCRDVLEVGASVLVKIRAKCSDGEVRFFGDDASRLDTLIDDSTA
                     GMRVHVSARGTDAEAIRIRLERSQQSGKGGKGGEITLVASLDGRREVEVRLPGRYRMD
                     AALRGALKSAPGVLMLEDA"
     gene            15854..15970
                     /locus_tag="VF633_13415"
     CDS             15854..15970
                     /locus_tag="VF633_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472103.1"
                     /translation="MAKGQVKSNKEVRKPKAEKAKPAVVVGTSPFTQVPGKK"
     gene            complement(16017..16523)
                     /gene="smpB"
                     /locus_tag="VF633_13420"
     CDS             complement(16017..16523)
                     /gene="smpB"
                     /locus_tag="VF633_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX8472104.1"
                     /translation="MAPTTKEKAAAAKSAPITKAKVIAENRRAKFDYFLEDNVEAGIM
                     LLGTEIKALRMGRANIAESYAAVEGREIVLINADIPPYAQANRFNHEPRRPRKLLLHR
                     KQIDRLIGAVQKDGQTIIPIRLYLNDDGKAKLEIALAKGKKLHDKREASADRDWARDK
                     ARLMRDKG"
     gene            complement(16523..17419)
                     /gene="dapA"
                     /locus_tag="VF633_13425"
     CDS             complement(16523..17419)
                     /gene="dapA"
                     /locus_tag="VF633_13425"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX8472105.1"
                     /translation="MTAPLFKGVITALITPLRDGNVDEAALTRLVERQIAAGIHGIVA
                     VGTTGEGGGLTIEEHKRVTSLCVDVAAGRIRVIAGAGSPATYESIDLAAHAKACGADG
                     SLVVTPYYNRPSQAGLVAHFEAVADAVQLPLILYNVPSRTSVDLSNESVAHLARHPNI
                     VAIKDATADMSRTSWMHANIVGKETQFDLISGDDHSFIGYAAHGGVGLISVTSNVAPE
                     AMVAMYDAVAAGDYATARSWQDRLIGLHKALFLDASPAPAKYALAKFGLCSEEMRLPL
                     TPTNDAVKPAIDAALATAGVTG"
     gene            17532..>18797
                     /locus_tag="VF633_13430"
     CDS             17532..>18797
                     /locus_tag="VF633_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8472106.1"
                     /translation="MLATTFAAALAVLVPLPQSTSELNGAQPYTAQPYTQAAGQGSTA
                     SYQPRVLNDQDTAAFRQGLAAARARNVVDTQTAIARIGDPTARKLVEWALVDTSAPQL
                     SFGELATDQQTLAGWPRADSRRAAGEIALDRAGLQPNEVLAFFGNSQPTTIQGAIALG
                     WALQQTGRTADAQRLIGDWWRTQSFDDASQSRILGQWSNWLTQADHEARLNMLLLGPH
                     GPATRSMLNLVSPERASIANAVMALRTAYSPDATVASLSASQAVNPAVVLERVRILRG
                     AGRGREGYELLRYLPAAPLHSEGQSTLWTERRNYYLDALQDRNWQAAYDAMNGHGFPG
                     GDRKVDAEFFAGWVALTKLNDPARAARHFEALRNTSSTPITQGRALYWLGRAAEAQGD
                     RNGAQQWYRAGSTHIQTFYGQLAAQKAGST"
BASE COUNT         3314 a         6016 c         6076 g         3391 t
ORIGIN      
        1 gggtctcgaa gaaccttgca tcgggcatga tgaagcgccc gtcgcggctc ttactggcct
       61 tgcggctgaa ccggaaccgg caggcggttg aagctcagcg taggcagttg ggtgttcagg
      121 cgcgcgatca cgtcggcagt cacgtccatg gcggggttca tgatgaaaac gctttcgcgg
      181 tccagcagaa tgccgcagcc cttttcttga tagatggcga cgagcagagg ctcgacggcc
      241 accgagatca ggcggcgctg ttcgctgagc gtgtaacgca gctcatcttc acgcgtctgt
      301 tgaagctgtt gagcctcctg ggcgcgctgt tgcagggcct gacggcggct ttgcaggtcg
      361 gcgggcgcga tcgtcgacgc tccttgctgc aaagcctgag cttcggtctg gatggttgtg
      421 gcgtagggct ggagttcgcc ctgcacttca gtggccagtt gacccatgcg agtctggacc
      481 gattggccgg cggtcgactg cgccagcagt tgctggttga agaacacgca gacgcccggg
      541 atggcaggcc cgacgttggc ggggccggcg gtttgggccg aggcggcgga agcgccgaga
      601 agcgaagcca gcgccgcagc gccgatgacg agggatttca tatcaagcct ttgagcggtc
      661 cgttcggacc tgcggttatt agaactgggt tgaagtcgag aaacggaacg attcagtacg
      721 gtcgtattct tcctgggacc agactttcga caggtcgaag cggatcggtc ccatcggcga
      781 tttccagtgg atgctgacgc cggatgcggc gcgcaacgac aggtcgtcga cgatgttggc
      841 gtcttgcaga cctgccgagg tcaaggtgta gcggtcgtcg agaaccccga gcgtaccgat
      901 gtcggtgaac agcgacgtct tgatgccgta agtctcgggc aggaagttcg gcagggttag
      961 ctcaacagtg cccacggcgt agaaattccc gcccagcgca tcattggtgg tcaggtcgcg
     1021 cggccccatg cctgcggttt cgaaaccacg gaacgtgttg ccaccgcgga agaagcggtc
     1081 gttgatccgc actgcatcgc cagcccaacc tgcgacataa ccgacggatc cattgacgct
     1141 aaccacccag gccggagtga tgccgtagaa gaccgaggct tcagcttcgg tcttgacata
     1201 gttcacgtca cccccgaggc ccgcgagatc ctgacgcatg gtcccggtcc agccgcgcgt
     1261 cggacgcacc gggtcgttgc ggcgatcgat tgcaagcgtg tagcccgccg aggagttcag
     1321 cgaggcgccg acctgatcgc aaagcgcgcg tgagccggat ccgccggtgg agcagtagcc
     1381 ggtcgggacg atgatctcat cgtccttgat gaagtagcgg gtgctgagac gggtgtagcc
     1441 gttgatcggg aacgacaggc gcaagccggc gccggtcgaa cggtagtcga acgacgactc
     1501 gtcctggaag tcgtagcggg agtggaacac gtcgaagccg gcgccgacgt cgcggccgag
     1561 gaagcgcggc tcggtaaagc ggaagtcgag ttgctgacgc aacgagcccc attcggcgcg
     1621 ggcgacgacg ttctggccac gaccgcggaa gttccgctcg gtgatgccca ggttgaccac
     1681 gaagctgtcg accgaggaga agcccgcccc gactgaaagc tcgccggtgg gctgttcctc
     1741 gacctggacg ttgaccacgg tccggtcagg cgccgaaccg cgcacttcct cgatcgtgac
     1801 atccttgaag aagccaaggg cgcgcagatt gttgcgcgaa cgctcgatca gggtgcggtt
     1861 gaaggcgtca ccttcggtca gcatcatctc acgacggatg accgggtcga tggtacgcgt
     1921 gttgcccacc acgttgatgc ggtcgatgta gacgcgctgg ccttcggaga tgttgaaggt
     1981 gacgtcgacc gtgttggttt cggcgttggc cttgtaggac ggcttgatgt cgacgaaggc
     2041 gtagcccgcc gaaccggcag cgaacgtcag cgcatcgacc gagctctcga tcttgtcgct
     2101 ctcgtagagc tggccttcac ggatcgggat cagcagcttg aggaagtcgg cgttcaggcg
     2161 actgttttcg gtgaccacgt tgatcttgcc gaaattgtac ttgtcaccct cgtcgagggt
     2221 catggtcagg ccgaacgcac tgttgttggg gaccagttcg gcgaccgccg agacgatgcg
     2281 gaagtcgtaa taaccgcggt tcgtatagaa cttgcgcaac tgctgctgat cgtaatccag
     2341 acggttagga tcgtagttgt cgttcgagct gaacaggcgg taccagcggg actccttcgt
     2401 gatcatgacg tcgcgcaggt cagtatcgga gaacgcggtg ttgccgagga tggtgatggc
     2461 ctgaaggccc gtttcgggac cctcgtcgat ctcgaacacg acgtctaccc ggttctgttc
     2521 aagctggacc agcttggggg ccacagtggc cgagattcgg cccgacaggc ggtacagctc
     2581 gatgatcttg ccgacgtctt cctgaacctt ggcgcgggta tagatgccgc gtggcgccag
     2641 agtgacttcg tcggtcagct tcttctcgga gagggcgctg ttcccctcga aagtcacctg
     2701 gttgatgatc ggattctcga cgatcgagac caccagatcg ccgttcggct gcaggccgat
     2761 ctggacgttg gcgaacagct gggtgcggtt cagcgtgcgg accgcgacgt ccagcaggac
     2821 ggcgtcgacc gtgtcaccgg gctggatcgg cagataggac agaaccgtcg tctggtcgat
     2881 ccgctggttg ccctcgacga ggatgcggtt gaccacgccg gtctcggcgg ggcgtggggc
     2941 cgcagctgcg gcaggagcca tttcgacagt gggattcgcc tgacccggag tcgtttgacc
     3001 gggggcagcg ggtgcggttg gcgccggggt cggagcggca gccgccggag cggtctgggc
     3061 catggcggtt ccgccaaggc cagccgcgat gacaagcgtc atcacactgc agagcggggc
     3121 tcggaagcgg gcggccatag cggcgggagc acgtcggtcg gtcatcatca tacgggaaac
     3181 catcgggggc tggcgttggc tcaactgacg agcccgccga ggaatttaaa caggttcagc
     3241 ttctgcaagt cgttccaggt cgcgaacaac atcaaacccg ccagcaaagc aagaccgacc
     3301 cggtatcctg cctcctgaac tctggccgcc acgggtttct gcgccacagc ctcataggca
     3361 taaaaaacca ggtgaccacc gtccagaacc gggatgggca tcaggttgat gaagccgatt
     3421 ccgatcgaaa gtatggcagc gagctgcacc attgtgaaaa ccaggttgat ggcgatcgca
     3481 acaggcgccc ggtctgcgcc aacggccgcg gtcgcgagtg cgcccgaggc cttggccatg
     3541 ccgatcgggc cgctgagttg gtcgccggac tcccggccgg tcaatatccg ccccagatat
     3601 ttcagcgtcg tcgacagggt atcgccgacc tgatgaacgc ccttgctcag cgcctccaac
     3661 ggattcaggc gacggaacgt gtagtccgaa cggatcatgg ggcgcagatc gagcccgatg
     3721 cgggacatac gcacccgccc ggcgatcttg tcgtccacca cctgccgtaa cggggttgcg
     3781 gtcaggctca cggaacggcc gccgcgatcc accgtgaagg tcaagggcgt gttggcgctc
     3841 agaagcacgg ttgaggtgac ttcctgaccg tcgcgcacag acctcccgtc gatcgctgtg
     3901 atcaggtcac gcgactggaa cccggctgcg gccgcaggag aaccggggag cacggcgtag
     3961 acacgtggcg ccacgatctg ctcgcctaca accccgaaca ggacggcgaa gagaacgatg
     4021 gccagcagga aattggcgac cgggccgcct gcggtgatca acgcccgctg ccacaccggc
     4081 ttgaagtgga aatagtcacg ctcggcgccc gggccgttct cggcgatgac gcggctgcgt
     4141 agttcggcga gaccagcctg atccggcacg ctggcggcgt cgaggtcgcc cgagaacttc
     4201 acatatccac caagcggcat ccagccgacg cgccattcca caccacgttt gtccgtgcgg
     4261 ctcagcagcg cccggccgaa gccgatggcg aaacggtcca tcttcacgtt gaacaggcgg
     4321 gcgatcagat agtggcccag ctcatggatg gtgacgatga acgtcagcac cagcaggaac
     4381 ggcacgatat agatcaggat ctgacccagg gcgctcaaca ttggaacgtc gtctccttcg
     4441 cctcaggcgg cgatagccag gccggcgacg atgccctccg cgatccggcg tgcttctgca
     4501 tccacggcca gggccgcatc gcacgcatcg tcgtaaccgg aattcatccc cgcgcttgtc
     4561 gccttatcaa gcgtttccgc gacgactgcg gcaatattga gaaaggtcaa ccgccgagcg
     4621 agaaacgcat aggctgcgac ctcatttgcc gcgttgaaca cagtcggcgc tgaccctccg
     4681 gccatcaagg cgtcacgcgc cagtttcagg gccgggaagc gtgtgtggtc gggcgcttcg
     4741 aaggtcaggc gcccgagggc tgcgaggtcc agcttcggcg ccggccactc cagacgatcc
     4801 ggatacgcca gtgcgcaggc gatcggcgtc ttcatatcgg gcggccccat ctgggccaat
     4861 gtcgagccgt cgacgtactc gaccattgag tggatgatcg attcgggatg caccacgaca
     4921 tcgatccgct cggccgccat accgaacaga taggcggcct cgatggtctc cagtcccttg
     4981 ttggccatgg tggcgctatc gaccgagatt ttggcgccca tgctccagtt ggggtgagcg
     5041 accgcctgtt caggcgtcat ccgcgccatc tcggctaatg acgtctggcg gaacggaccg
     5101 cccgacgccg tcaggattaa tttcgccacc cgttcgggcg catcggccgg aaaaacctga
     5161 aagatggccg aatgttccga atcgaccggg acaagccgcc cgccactggc cttgacccgc
     5221 gcgatcaggc tcatgccgca gcagaccaga ctctccttgt tggccagtgc cagggtggcg
     5281 cccgtcccag cagcggccca ggtcgaacgc aacccggccg ccccgacgat cgaggccatg
     5341 atccagtcgg ctggacgcgt cgcggcctcg atcaccgcac cgtcgccccc agccacctcg
     5401 atggcgagat ctgcaagagc atcacgcaaa gcgccgagac ccgccggatc ggcgatgacg
     5461 acctgtttcg gccgccacct gcgcgcctgt tcagccaacc gggcgacatt cccgccgcca
     5521 gtcagaacct cgacatcgaa gcttcctgtt ccggccgcct cggcctgggc catcagctcc
     5581 agcgtcgaac agccaaccga gccggtcgac cccagcacgc tgacacgacg gcggatcaag
     5641 cagcgcgctc caccagcagc accagccatc gcccggcaga cacgatcacg accgcgaaca
     5701 tcaatccgtc cacccgatca agcagtccgc cgtgaccggg gatcaggttg ccggcgtctt
     5761 tcacgccgaa ccggcgcttg agagccgatt cccacaggtc gccccccatg gtggccagcg
     5821 ccgcagcaag gccaagcaca ccgccccaaa agatgttcag ccgacccatt tcaagccacg
     5881 cggccattcc ggctccggcg atcatcccgg cgacgaggcc gccgatgaag cccgaccagg
     5941 tcttgttggg cgaaaagcgg ggccacagct tgggcccgcc cacggcgcta ccgaccagat
     6001 aggccaggat gtcggcagac caggcgacgg ccagaaccag cacggtccag tgcaacccga
     6061 ccggggaatg attgtcgcgc aaccagacca gcaagacggc gggccagccc agataaagaa
     6121 cgccataggc cgcgttcagt gcttcctgcc cccggctttt tgcgaacact cccgccgcgc
     6181 ctgcgccaaa caccagcagg acgagcgcca gcgacaactg cccgacgtga gtcgttacga
     6241 ccgcggcgaa cagacccagc gccacggcgc aggtcatcat ccgccaggcg cccggtgcgc
     6301 tcatcatggc ccattcgacg gccaggaggg cgcaggccgc cagtatcagg gcaaggaacc
     6361 acacaccgcc ggcccatgtc gccagcacag ccgccggcgc cagaacgaag gctgacgctg
     6421 cacgaatacc gatgtcgccg accttgagcg ccatcagccg gtcgcaagcg ccggctgggc
     6481 catgatcccg ccaaaacggc ggtcacggtt cagatattga tctacagctt cgccgagcgg
     6541 accggcgccg tagtccggcc aaagcacgtt ctggaagaca agctcggcat atgcggcttc
     6601 ccaaagcagg aagttcgaca acctctgctc accggatgtg cgcacgatca ggtcgacggg
     6661 cgggccgcct gcggtagaca ggcccgcacc tagcatatct tcgtcgagcg gcccgctcgc
     6721 ctcgcccgcc aggacgcgat cgacatgccg ctgcgccgca tcgacgatgt cggcgcggcc
     6781 gccgtagttg aaggctacct gcagcaggaa gcgactgttg tgggctgtct gggcctcggc
     6841 gcgttcgatg attgccgcga tgtcggtcgg aaggccagtg cggcggccga gcactcgaac
     6901 ccggacgccc tcgcgttcca gcctgttcag gtcgctggcc acataggacc ggaccagccc
     6961 catcaaatcc gagacttctt ccgccgggcg gcgccagttc tcggtcgaga agccgaacac
     7021 cgtcagacac tctatgccga actgcggcgc ggcctgaacg gtgcgcttca gagcctgaac
     7081 gccctcacgg tgccccatgg cgcgcggaag gccccgggcc tgcgcccagc ggccattgcc
     7141 gtccatgatg agcgcgacat gcttcgggcc ttcagaagtc ccgggcagat cggcggtgaa
     7201 agccgtcatt gctgtgcgtc ctggcaggat ggatacgagc cgaaactcag acctgcatga
     7261 tctcgacttc cttggtcttc aaggtctggt cgatgagttt gatagcggag tcggtgtcct
     7321 tctggacctc ggcctccatt ttcttctgtt cgtcctggct tatctcgcca gccttttcag
     7381 ccttcttaag atcatcgttg gcgtcgcgac gcacgttacg cacggaaatc ttctgctgct
     7441 cggcgtattt gccagccagc ttggcaagat ccttgcgccg ctcctcggtt agcggtggca
     7501 cagggatacg caaagtctga ccatcgacga tcgggttaag tcccagacca gctgcgcgaa
     7561 tggccttctc gactggcccg accatggttt tgtcccagac gctgaccgaa atcatgcgcg
     7621 gctcgggtac gctgatcgcg gccacggcgt tgagcggcga gtttgaaccg taggcctgga
     7681 cctgaaccgg atccagcagc ccggcatggg cacggccggt gcgcagtccg gagtattcct
     7741 ccttcagcga cgaaatcgcc ttgtccatcc gctcgcggta ggtcttcaga tcgggcttgg
     7801 ccatggcgtg tctcctagtc tcttatagtc aggcggcgac gtcgttgtcg ctgatgaccg
     7861 tgaaggtccc ctgcccgttc agcactttac gcagcgaatc ctcttcccgg atagagaaca
     7921 cgacgatcgg gatccggctc tctcgcatca gggcgatggc ggcggcgtcc atgaccttca
     7981 ggttctgcgt caggacgtcc tgataggtca ggtgctcata gcgggtggcg gtcttgtcct
     8041 tcttggggtc ggccgaatag acgccgtcga cgctggtgcc cttcagcaga gcatcgcagt
     8101 tcatttctgc ggcccgcaag gcagcagcag tgtcggtcgt gaaatagggg tttccggtcc
     8161 cggctgcgaa gatgaccacg cggcccttct ccatgtgccg cacggcacgg cggcggatgt
     8221 agggttcagc gatggcggcc atcggaatgg cgctttgtac gcgcgtttgg acgccgattt
     8281 tctcgagcgc attctgcatg gccagggcgt tcatgaccgt ggcgagcata cccatatagt
     8341 cggcgctggc gcgttccatg tcggctgccg ccttggacag gccgcgaaag atattgcccc
     8401 cgccgatcac caggcagatc tcggtcccgg actccgcaac ccgctttatg gccgcagcga
     8461 ccccgtcgac ggtatgcatg tcgatgccat agccctgctc gcccatcagg acctcacccg
     8521 agaccttgag caacacccgt ttgtattttg gaccggcagt agcgtcagac atgggggagc
     8581 tcttttcgcg aggaggcgcg gaatcaggtg ggcgtatcat agacgaaggg cgcgtccggc
     8641 ttcaaagccg aacgcgcccc gttagtcacg taataagtat ggtttaggct tgcggagcca
     8701 tcatcgacgc gacttccgaa gcgaagtcgg ggccttcgac cttctcgacg ccttcaccga
     8761 gagccaggcg cacaaagccg gcgaggtgca ggttggacga gccgagttcc ttggccgagt
     8821 tggcgaccag ctgttcgatg gtcacgtccg gatccatgac gaacggctgc ttggtcagca
     8881 cgacgtcctt ctggaacttg ttgatctggc cttccacgat cttggcgatc atgttttccg
     8941 gcttgccttc ttccttggcc ttttcggtca ggacggagcg ttccttctcg atggccgcgg
     9001 ggtccaggtc gtcggtgttc agcgacagcg gagcggtcgc tgcgacgtgc atggcgatct
     9061 tgcggcccag ttcacgaaga gcggtcttgt cgccctcgcc ttccagagcc accagcacgc
     9121 cgatacggcc gaggcccggc gcgacggcgt tgtggacgta ggaggcgaca acgccttccg
     9181 acaccgacag gcgagcggcg cgacgcagtt gcatgttctc gccgatggtg gcgatcatgc
     9241 tggtcacctc gtcctgaatc gtcttgccgg cttccagctc ggcgccgtgc agggcttcga
     9301 cgctgtggtg ctccagaccg tgctcggcga acgccttggc ggcgttctgg aacagctcgt
     9361 tacgagcgac gaagtcggtt tccgagttga actcgatggc ggcgccgatt tcgcccttgc
     9421 cgtcttcacg gctggcgacg gcgacgaggc cttcagcggc gacgcggtcg gccttcttgg
     9481 cggccttgga caggcccttg gcgcgcaacc agtcgatcgc cgcgctgacg tcgccgtcgg
     9541 tttcctgaag cgcctttttg cagtccatca tgccgacgcc tgacttggcg cgaagttcca
     9601 tgacgagggc ggctgtgatc tcggccatat tagtctcctg agtatctgta tgcggtaacg
     9661 gccggacccg cgaagggtcc ggccgtgtgt tgatttccgt cgatgacagt cccgaaggac
     9721 taagccgtgg cttgttcggt ctcgacggcg tcgttcgctt cggtagtagc ttcttgagcc
     9781 tcgccggaag cgaccagcat ttcctcggcg acggcttcgg tgcctgcagg ggcgacgtca
     9841 gcagcgaccg gagccgaagc ttcggccagc atcggctcga cggggttctc cgaagcgccg
     9901 aggtcgacgc ccgagttgga ggcgccggcg gccagaccgt cgaggacggc gtcggcgatc
     9961 aggtcgcagt aggtctggat cgcgcgagcg gcgtcgtcgt tgccggggat cggataggtg
    10021 atgccgtccg gatccgagtt ggtgtccaga atggcgatga ccgggatgtt cagcttgcgg
    10081 gcttcaagga tagcgatggc ttccttgttg gtgtcgatca cgaacatgat gtcggggatc
    10141 gagcccatgt ccttgatgcc gccgagcgac agttcaagct tgtccttctc gcggccgagg
    10201 ttgaccagtt ccttcttgac ccggccttcg ccgccgcgct ccatgatgac ttccagctcg
    10261 cgcaggcgag cgatggagcc cgacacagtg cgccagttgg tcagggtgcc gccgagccag
    10321 cggtggttca cgtagtactg ggcgcagcgc ttcgccgctt cagcgacggg atccgaagcc
    10381 tggcgcttgg tgccgacgaa cagaacgcgg ccacccttgg cggcgacgtc acgcacggcc
    10441 accagagcct ggtggaacag cggcatcgac tgcgacaggt cgatgatgtg gatgttggag
    10501 cgcgagccaa agatgtagcg ttccatcttc gggttccacc ggtgcgtctg gtggccgaag
    10561 tgggcgcctg cttcgagcag ggtgcgcatg gagaattctg gcagagccat ttggaagtcc
    10621 tttttccgat tattccgccg cagacggtga aagagcctga tggctcctcg gaacggcagg
    10681 gaccgggatg tctccccgac ccacgccacg tctgcgtgtg aagtgggcgc ggatgtagtc
    10741 gggttgggta gaaaaggcaa gcggtatggg gcatgaacct gatccgctca tccccgtcaa
    10801 agccggtacc cggtagcggc tcacagtctg cggcgtcctg caggggcctt cgctcccgcc
    10861 cccgagaacg caagaaaggc ggccccttgc ggaaccgccc ttctcgagtc tgaagatcgg
    10921 acgcttctag ttcatagcgt cgagcatggt cttggcgtgg tcgcggtgca tggtgacctt
    10981 ggggatgatc tccttggcca gaccggccag cgtcggggcc tcggtgttgt cggagaagcc
    11041 gttcagcagg gtcagggcct cgttatgggc ggcgacctgc tgggtcagat agaccttgtc
    11101 gaagtcggcg gccgaggcgg cgttcaggtt gtcgatcatt ccctgacggc gttcgtccag
    11161 cgtagtcgga agagtcacgc ccggggcctc ggtcggtgcg gcagctttca gcttggtcga
    11221 ggaagccgtg tggtccttcg tgatcatggc cgccaactcc ttgatgtcgg ccttgctcga
    11281 tttcgaggcg gcgatcttcg cgccctccag ctcgtacatg tcggacatgg ccaggctggt
    11341 cgcgaagccg ccgacggtgt tggcgccgag tgtggcggcc gaggcctggc cgacggcgcc
    11401 ggcggtggca tcctgcgtgg cgttgacggc ggcgcccgca tctccggcgg tcttgtcggt
    11461 ctgttgctgg ttacaggccg ccatcagggc ggcgacggcg acgaccgcga gtgcgggttt
    11521 ggcgaaggtt ctgatcatat gtggtctccc ttggttgccg gagaaaccgc tgcgctgaag
    11581 gtttgttcct gtgtccgtta acggacagat cgccgcgcca tcaaccggta cgatcaggcc
    11641 gttcgatcag ggttgaggca ggctgtactc aagatcgtag cggtgctcgc cgccctcgat
    11701 cgtcaggtgg aagacgcctg taatcccctg gagatcgtcc gttcccgatc ccggcacaac
    11761 ggtgatcaga aggtcctgcg cgcccttgtc catcaggcct cggtgaacca gcgcaaagct
    11821 gcccgagcga ccctcgagcg taccgttgac ccgttccatc gcgacataag cgccggaagc
    11881 gcccggagcc atggtcgcca gcatttcgcc cgtcgcagtc ccgctcagat cgccgtggaa
    11941 gaccttggac agggtcatgc gcccgggaac atcggctgca gtcccagcag aaggctggac
    12001 gggagtgatc gtaacatcga aggttccgcg cgcgtggtgc atgtctgtcg tctcggtcgt
    12061 cgaggtctgg gctgccagca tcattaccgc agcggcgagt atcgaagtga acatcaggcg
    12121 tcttccagca tcagcacgcc gggcgcggac ttgagcgcac cccgcagggc ggcgtccatc
    12181 cggtaacggc ccggcagccg cacctcgacc tcgcgacggc cgtccagcga ggcgacgagg
    12241 gtgatctctc cgcccttgcc ccccttcccg ctctgttgcg agcgttcaag ccggatgcgg
    12301 atagcctcgg cgtccgtgcc tcgtgccgag acgtggaccc gcatcccggc ggtcgagtcg
    12361 tcgatcagag tgtccagccg tgaggcgtcg tcaccgaaga accgcacctc gccgtccgag
    12421 catttggccc tgatcttgac cagcaccgac gcgccgactt ccagcacgtc acggcatttg
    12481 cgtaactgct ctggcgggaa caggcattcg aactcgcccg tgggatcgga gaagttgacg
    12541 aaggcgaatt tctcgccggt ccgggcgctg gccctttcct gacgacggcg gatgacgccg
    12601 gccatcatga aggcttcgtg gccgctttcg gccagggccg tagcctcggc gacgaaggtg
    12661 acgcgcttgc gtttcagcgc cgggatcatg tcctcgagcg ggtggcccga cagatagaag
    12721 ccgacggcgg ccaactcatg gtccagttgt tccgggccga tccatggatc gacgctgcgc
    12781 aaccggggcc gggcggcatt ggcctgatcg gcgccgaaca acgacacctg cgacgacgcc
    12841 cgttcagccg caacgctctg gcaataggcc atcagcacat cggcctgctc cagcaactgc
    12901 cgacggttcg ggtggatggt gtcgaacgcc ccggccttgg ccagaccttc caacgcccgc
    12961 ttgttgaccg cgcggggatc gacgcgttcg aggaaatcga agatgtcggc gaaccgcccg
    13021 ccggtctcgc gcacctcgac gacgtgcttc atcgcctcga ggcccacgtt gcggatggcg
    13081 ccgagcgcat agaggaccgc gcccttctcc tcgctccaag cgacgtcgaa gtcggctgac
    13141 gaccggttca catcgggcgc catcaccggc acctcgaact tgcgtgcgtc ctgatagaag
    13201 accgccagct tgtcggtgtt cgacaggtcg agcgacatcg aggcggcgaa gaactccacc
    13261 ggatggttcg ccttgagcca gccggtctgg tacgaaatca gtgcataggc ggcggcgtgg
    13321 gatttgttga agccgtagcc ggcgaacttg gccaccagct cgaagatcga gtccgactgc
    13381 tgctccggca cggacttctc ggacgcgccc ttgatgaagc gcgccttctg gaaatccatc
    13441 tcctccttct tcttcttgcc catcgcgcgg cgaagaaggt cggcctcgcc gagcgaatag
    13501 ccggccagga cctgggcgat cttcatcacc tgttcctgat agatgatgac cccgtaggtc
    13561 tcggtcagca cctccttgag cgaggggtgc agatagtcga cctcggcccg accgaactta
    13621 cggtcgatat aggtgtcgat catctccatc gggcccggcc gatagagcga gatcagcgcg
    13681 gtgatctcct cgatggagcc gcaacgcatc ttgcgcaggg tgtcgcgcat gccttgcgac
    13741 tcgagctgga acaccccgac cgtctggccc gaggccatca gctcgtaggt cgccttgtcg
    13801 tcgagcggca gctggttcca gactggctcg gccccgcgcc gcgacaggta ttcgcgcgcc
    13861 cggtccagcg tcgtcagggt cttcaggccg aggaagtcga acttcaccag acccgccggc
    13921 tcgacccatt tcatgttgaa ctggctggcc gggatggtgg agcgcgggtc ctgatacagc
    13981 ggcaccagtt cgaccagcgg ccggtcgccg atgacgatgc cggcggcatg tgtcgaggcg
    14041 ttgcggtaga ggccttccag ctccagcgcc gtctccagca gcgtgcggac cgaggcctcg
    14101 gaatcgcggg cctccttcag ccgcggctcc agatcgatgg cttgagccaa ggtcaccggc
    14161 gccgctgggt tattcggcac catcttgcag agccggtcga cctggcccag cggcatctgc
    14221 agcacccgcc cgacgtcgcg cagcacggcc cgcgcctgca gggtgccgaa ggtgatgatc
    14281 tgggcgacgc ggtccttgcc gtagcgcgcc tgcacgtagt cgatgacctc ctcgcgccgc
    14341 tcctgacaga agtcgatgtc gaagtcgggc atggagaccc gttcagggtt caggaaccgc
    14401 tcgaacagca agccgaaccg caaagggtcg aggtcggtga tggtcagggc ccaggcgacc
    14461 agagacccgg cgccggaacc ccgccccggt ccgaccggga tgccgtggtt cttggcccat
    14521 ttgatgaagt cggccacgat caggaaatag cccgggaacc ccatctgctg gatgatcccg
    14581 acctcccatt cgaggcgggt ccagtactcc gcctcgggca tcgccggagt gacttccttg
    14641 agccgaacct tcagcccctc gcgggcctga tacgccagct cgtcaggctc ggtgcgcccg
    14701 gccccggcgt cgaaacgcgg cagtatcggc gcgtgtgtgt tgacgaggaa ggcgcagcgg
    14761 cgggcgatgt cgatagtgtt gtcgcaggct tcgggcaggt cggcgaacag ggtccgcatg
    14821 gcctcgaccg acttgaacca gtgctggtcg gtgatccggc gccggtcttc ctgaccggtg
    14881 aaggcgccgt cagcgataca cagcagggcg tcgtgcgatt tcgcctgcgc ctgtttggcg
    14941 taatagacgt cgttggtggc cacgatggga acgtcgttgg cataggccca ggtcaccaga
    15001 ccctgttcgc cctgcccttc gtgcggcagg ccgtgacgct gcagctcgac atagaagcgg
    15061 tcaccgaaca cgcgcgccat ctcggccaga gccgcatcgc cctcggcgtt cttgttctgc
    15121 acatagagcg gatcgaccgg gccgtccgga ccgcccgaaa gcaggatcaa gccctcggaa
    15181 tgggccacga tcttgtccca cggcacgccc ggctcattgt cgccggcatc cagataggcc
    15241 gacgacgaca gggcgcacag attgcgccag ccggcctcgg tctgacagat cagaaccacg
    15301 gtagggacct tggcccaacg ctcgcccgcc ttgccgccga tacccatgac cggcatggcg
    15361 caggccacca gcggctgaac ccctgcgccc ttcgaggctt cggagaactc cagcgccccg
    15421 aacaggttgg cccggtcggc cacgcccacg gcgggcatcc ctgagtccgc cgccagtttc
    15481 gggatctttc ccgacttcat cgcgccctcg agcagcgaat aggccgagcg aaccctaagg
    15541 tggacgaaac ccgtgctgga aaccgtatcg gtgatggcct gatcctcaga ggcaggccgg
    15601 aaacgactca tgtcgccagc agggccgcct gccagaccat ccagacaaac ccgccgcagg
    15661 atgcaagcga cagcatatct ctcaccattc tcgacataga cttatcccca gactgtatgt
    15721 tccggtattg ttctatgttg gctatttgtt ctcgtcaaca gccttcgata tgatgggaca
    15781 gaatgaggga cgccgggtat tctgatatca atgcccaccc accatcccgg tggcaggcgt
    15841 gaggagagcg gtcatggcca agggtcaagt caagagcaac aaggaagtcc gcaaacccaa
    15901 ggcggaaaag gccaagcccg ccgtcgtggt cggcacgtcg ccgttcaccc aggttcccgg
    15961 caagaagtag tcggcggggg ctcggccccc accttcccgc gccctaggcc taagtcctag
    16021 cccttgtcgc gcatcaggcg ggccttatcc cgcgcccagt cgcggtcggc ggaggcttcg
    16081 cgcttgtcgt ggagcttctt gcccttggcg agggcgatct cgagcttggc cttgccgtcg
    16141 tcgttcagat agagccggat cgggatgatg gtctggccat ccttctggac ggcgccgatc
    16201 agccggtcga tctgcttgcg atgcagcagc agcttccgcg gccgacgcgg ctcgtgattg
    16261 aaacggttgg cctgggcata gggcgggatg tcggcgttga tcagcacgat ctcgcggccc
    16321 tcgaccgccg catagctctc ggcgatattg gcccgcccca tgcggagggc cttgatctcg
    16381 gtccccagca gcatgattcc agcctcgaca ttgtcctcga ggaagtagtc gaatttagcc
    16441 cgccggttct cggcgatgac cttggccttg gtgatcggcg cgctcttggc ggcggccgcc
    16501 ttttccttcg tcgtcggggc catcagccag tcactccggc agtcgcgagg gccgcgtcga
    16561 tggccggctt gaccgcgtcg ttggtgggcg tcagcggcaa tctcatttcc tcactgcaga
    16621 gcccgaactt cgccagcgca tatttcgccg gggccggcga tgcgtcgagg aacagcgcct
    16681 tgtgcagtcc gatcaggcga tcttgccacg agcgcgccgt ggcgtagtcg cctgcggcga
    16741 ccgcgtcata catggccacc atcgcctccg gcgcgacatt tgaggttacc gatatcaaac
    16801 cgaccccgcc gtgggccgca tagccgatga agctgtgatc gtcgcccgag atcaggtcga
    16861 actgcgtctc cttgccgacg atgttggcgt gcatccagct ggtccggctc atgtcggccg
    16921 tcgcgtcctt gatcgcgacg atattgggat gccgcgccag gtgagccacg ctctcgttcg
    16981 acagatcgac gctcgttctc gacgggacgt tgtacaggat caacggcagc tgaaccgcgt
    17041 ctgccacggc ctcgaaatgc gccacgagcc cggcctgcga aggacggttg tagtaggggg
    17101 tgaccaccag cgaaccgtcg gcaccacaag ccttggcgtg ggccgccaga tcgatcgact
    17161 catacgtggc gggagacccg gcgccggcga tcacgcggat ccgccctgcg gcgacatcga
    17221 cgcacagcga ggtcacgcgc ttgtgctcct cgatggtcag gccgccgccc tccccggtcg
    17281 tgcccacagc gacgatgccg tggatgcctg cggcaatctg gcgctcgacg aggcgcgtta
    17341 aggcggcttc gtccacgttt ccgtcacgaa gtggtgtgat cagggcggtg atcacgccct
    17401 tgaacaaggg ggcggtcata ggaacaactc tacctgcgtg ggaaatgggc gacgcgcgcc
    17461 tgtaacgagc gatagagggt aggttgccgg cttggctgct gcaactgcga tgaatgggga
    17521 tctttaaaac catgttggcg accacctttg cggccgcgct tgcggtcctc gttccgctgc
    17581 cccaatcgac gtctgaattg aacggcgccc agccgtacac ggcccagccc tatacgcagg
    17641 cggcgggaca aggttcgacg gccagctatc agccccgcgt gctgaatgat caggacactg
    17701 ccgccttccg tcagggactg gccgctgccc gcgcgcgcaa cgtcgtcgac acccagaccg
    17761 cgatagcccg catcggcgac ccgactgccc gcaagctggt cgagtgggcg ctggtcgaca
    17821 cctcggcgcc gcaactgtcc ttcggcgaac tcgcgacgga tcagcagacc ctggccggct
    17881 ggcctcgcgc cgacagccgc cgcgccgccg gagagatcgc gctggatcgt gccgggctcc
    17941 agcccaacga ggttctcgcc ttcttcggaa acagccagcc gaccaccatt caaggggcca
    18001 tcgccctcgg ctgggccctg cagcaaaccg gccgcacagc cgatgctcaa cgattgattg
    18061 gcgactggtg gcggacccag tcgtttgacg acgccagcca gagccgaatc ctcggccagt
    18121 ggagcaactg gctgacccag gcggaccacg aggcgcggct gaacatgctt ctgcttggcc
    18181 cgcacggccc ggcgacacga tcgatgctca atctggtttc acccgaacgc gcgagcatcg
    18241 ccaatgccgt catggccctg cgcaccgcct acagccctga tgccacggtc gcgtcgctga
    18301 gcgcgagcca ggcggtcaat ccggctgtgg tgctcgagcg tgtgcgtatc ctccgagggg
    18361 caggccgtgg acgcgaaggc tacgaactgc tgcgctatct gcccgccgcg ccgttgcatt
    18421 cggagggcca gagcacgctg tggaccgagc gccgaaacta ttacctcgac gccctgcagg
    18481 atcggaactg gcaggcggct tacgacgcca tgaacggcca cggtttcccg ggcggcgacc
    18541 gcaaggtgga cgccgagttc ttcgccggct gggtggccct gacaaaactc aacgatcccg
    18601 cccgcgcggc gcgccacttc gaggcgctac gcaatacgtc ttccaccccg atcactcagg
    18661 gccgagccct ctactggcta ggccgcgcgg ctgaagcgca gggtgaccgc aacggggccc
    18721 agcaatggta tcgagccggg tcgacgcaca tccagacctt ctacggccag ctggccgccc
    18781 agaaggccgg ctctacg
//
LOCUS       DASVTM010000485         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_151008,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000485 DASVTM010000000
VERSION     DASVTM010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151008"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..822)
                     /locus_tag="VF633_13435"
     CDS             complement(76..822)
                     /locus_tag="VF633_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8472107.1"
                     /translation="MNIAQDTVLITGGGAGIGRAFAEALHAKGAKVIIAGRREGPLQE
                     VVAANPGMAYVVLDVADPEAIKTVAARVVADHPNLNAVVNNAGIMKTEDLSGGVIDLS
                     VVEETLAINLLGTIRLTAALLPHLLTKPAATVVTVSSGLAFVPLAATPTYNATKAAIH
                     SWSQSLRHQLRDTSVKVVEWAPPAVATDLMPGHAENPNSMPLADFISESVALFEAGND
                     EVQVERVKFLSGAEGRGEYAKVFGIVNGAH"
     gene            926..1327
                     /locus_tag="VF633_13440"
     CDS             926..1327
                     /locus_tag="VF633_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8472108.1"
                     /translation="MSAPALAHPKPEYPPVDPRVEALVNEVIGRLADKWTMIVLDVLA
                     ENGELRFTRIGQLSDGISQKMLTQTLRAMERDGLVVRTVHPVVPPKVEYRLTDMGFSL
                     AEAFCGVWTWAGEHLERIETARAGFDAAKAG"
BASE COUNT          290 a          497 c          594 g          303 t
ORIGIN      
        1 ggggcgagat tatcagggtc gagtgtctcc acccttttgg gctgatcccg gcggtgcggg
       61 atcggcgggg ttggttcagt gcgctccgtt cacgatcccg aacaccttgg cgtactcgcc
      121 ccggccttcg gctccggaca ggaacttgac ccgttcgacc tggacctcgt cattgcccgc
      181 ctcgaacagc gcgacgcttt cggaaatgaa gtcggccagc ggcatggagt tggggttctc
      241 ggcgtggccg ggcatcaggt cggtggcgac ggcgggaggc gcccattcga cgaccttgac
      301 cgacgtgtcc ctgagctggt ggcgcaacga ctggctccac gaatggatgg cggccttggt
      361 ggcgttgtag gtcggggtcg cggcgagcgg cacgaaggcc agacccgacg atacggtgac
      421 gaccgtcgcg gcgggcttgg tcagcagatg gggcaacagg gccgccgtca gccggatggt
      481 gcccaacagg ttgatggcca gcgtctcctc gacgacggac aggtcgatga cgccgcccga
      541 caggtcctcg gtcttcatga tcccggcgtt gttgacgacg gcgttgaggt tcgggtggtc
      601 ggcgacaaca cgggccgcga cggtcttgat cgcctcggga tcggcgacgt cgaggaccac
      661 gtaggccatg cccggattgg cggcgacgac ctcttgcaac gggccttcgc ggcggccggc
      721 gatgatgacc ttggcgccct ttgcgtgcag ggcctcggcg aaggcgcgac cgatgccggc
      781 gccgccgcca gtgatcagga cggtgtcttg ggcgatgttc atgggaggtc tccgggctgg
      841 aacttcgaca tccggcgatg tatgacggtc gctctccatt ggaaagtagg caccgaaagg
      901 ttcggtactt accggaagga aactgatgtc agcgccggct cttgctcatc ccaaacccga
      961 atacccgcca gtcgatcccc gtgtcgaagc gctggtcaac gaggtcatcg gccgcctcgc
     1021 cgacaaatgg accatgatcg tgctggacgt cctggccgag aacggcgagc tgcgcttcac
     1081 ccgcatcggc caactctccg atggcatcag ccagaagatg ctgacccaga cgttacgcgc
     1141 gatggagcgc gacgggctgg tggtgcgcac ggtccacccg gtggtgccgc ccaaggtcga
     1201 ataccgcctg accgacatgg gcttcagtct ggccgaggcg ttctgtggcg tatggacgtg
     1261 ggccggcgaa catctggagc ggatagagac ggcgcgggcg gggttcgatg cggcgaaggc
     1321 ggggtagggc ggagaccgga cttcgccgat gcctgccagg ggtggttagg ggacgttgat
     1381 cgtggtcatc tcgtcggatg gcgatcaaca cacaggttca cgacctccgg cctgacggcc
     1441 ttggcggaat atgggcgagg gtcatcgttg atgttcctct tgcagacagg cctcagctaa
     1501 gcgacgccat cgacatcaac ctgcatctac ccggcgcagc cagcctcaca tttgcggagg
     1561 cggaggagcg ggctaagcgg gacgctcacg ctttactgaa gagggtcgcc gaaaacaggt
     1621 gaatgtccgt tgagggtcaa tttgaggagt tgccttcagg ccgcaaagcg gacgttgccc
     1681 tcgc
//
LOCUS       DASVTM010000486         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_259895,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000486 DASVTM010000000
VERSION     DASVTM010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259895"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VF633_13445"
     CDS             complement(<1..695)
                     /locus_tag="VF633_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8472109.1"
                     /translation="MSGEPSIFFADVNERQGSFLRRTFVVGGLTALGIGALGARLAQL
                     QVVQAKEYATLATNNQFNFRLVPPPRGRILDRNGVVIAGNRPSFRVLVVRDETKDLDQ
                     TLDLLGRLLPDTIERRRTIIREVNAARPSSPVPVKSDLNWEEFAKVNLYGSDLPGVMT
                     DMNEARFYPFGGSFAHVIGYVAKVTDRDIEGMKKRGETPPEILYNPGFRIGRSGIEKA
                     LDADLRGEAGGTRV"
     gene            complement(692..1210)
                     /locus_tag="VF633_13450"
     CDS             complement(692..1210)
                     /locus_tag="VF633_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472110.1"
                     /translation="MKRSAAVRVVGPVEWIVYPSLIVIAATLVLATPVQLFGLKLPEP
                     VLPMILAFAWPLVRPSMIAPAVLFFVGLFIDLLWGGMLGVWPLSLLAVYGAVLLSRNL
                     LAGQETQLLFVWYGACTLLAFFVTYLIVSLDAGNAPSVLALLGQMIPTLLLFPVANWM
                     IQRFDDGDTRFR"
     gene            complement(1207..2352)
                     /gene="mreC"
                     /locus_tag="VF633_13455"
     CDS             complement(1207..2352)
                     /gene="mreC"
                     /locus_tag="VF633_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX8472111.1"
                     /translation="MAFRDGPFENLKVPLAWTAAVAVIVALIASLFLIVSDRREVRDH
                     DNYTPVRAGFDSVAGPVNGVLAAPVRWTGALSDGIKGYFFAVSENRRLRAQVADLQPW
                     RDQAIALKNVNARYEAMLGLRTEPAVAMVTARSVSDARGPFVNARLIDAGSSKGVRIG
                     NPVINEHGMVGRIIGTTGGISRVLLLTDVSSRTPILIDRTDARAMLTGDGSGNPRLEF
                     LRSRDLQAGDRVLSSGDGGGIPRGVPIGVAAKGIDGSWRVKLFSDRGAIDYVRVMLFQ
                     DFGQLVNPEALNAAPLAGLGTAPSPGSAQAAAIGDASARRAAAAASAVERARAASATP
                     PAATSPAAIAAAPRPTAPRPVAPRPTQAAPGATPTGVPAPTQPGGAL"
     gene            complement(2515..3555)
                     /locus_tag="VF633_13460"
     CDS             complement(2515..3555)
                     /locus_tag="VF633_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8472112.1"
                     /translation="MFSHLFGAISNDIAMDLGTANTLIYMKGKGIVLNEPSVVALRNV
                     GGRKIVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKHFIRKVHNRRGF
                     VNPKIIVCVPSGATAVERRAINDSCLNASARRVGLIDEPMAAAIGAGLPIHEPTGSMV
                     VDIGGGTTEVAVLSLSGIVYSKSVRVGGDKMDDSIISYMRRNHNLLIGETTAERIKKD
                     IGTARMPADGEGLFIEVKGRDLMQGVPREVRISERQAAEALAEPVAQIIDAVKVALEA
                     TPPELAADIADKGIMLTGGGALLRGLDAEIRDHTGLPVSVADDPLSCVAIGCGRVLEH
                     PRWMKGVLDSAL"
BASE COUNT          655 a         1161 c         1189 g          578 t
ORIGIN      
        1 acacgcgtgc cgcccgcttc gccacgcaga tcggcgtcca gggccttctc gatgccggac
       61 cgcccgatgc gaaagccggg attgtagaga atctcaggcg gggtctcgcc acgcttcttc
      121 atcccctcta tgtcgcgatc cgtgaccttg gcgacgtagc cgatgacgtg tgcgaacgat
      181 ccgccgaacg gatagaagcg cgcctcattc atgtcggtca tgacccccgg caggtcggag
      241 ccatacagat ttactttggc gaactcctcc cagttcaggt cgcttttgac cggaaccggt
      301 gacgacgggc gagcggcgtt gacctcgcgg atgatcgtcc tgcgccgctc gatggtatcg
      361 ggcagaagcc gtcccagaag gtccagcgtc tggtccagat ccttggtctc atcgcgcacg
      421 accagcaccc ggaaactcgg acggttaccg gcgatgacga caccgttgcg atcgaggatg
      481 cgcccgcgtg gcggtggcac aagacggaag ttgaactggt tgttggtggc gagggtcgcg
      541 tattccttgg cctgaaccac ctgaagctgg gccagccgcg cccccagcgc gccgatgccc
      601 aacgccgtca gcccgccgac aacgaaggtt cgacgcagga acgagccttg gcgctcattg
      661 acgtcggcaa agaagatcga gggttcgcca ctcatcggaa acgcgtgtcc ccgtcatcga
      721 agcgctggat catccagttg gcgacgggaa acagcagcaa ggtcggtatc atctgtccaa
      781 ggagcgccag aacgctgggc gcattgcccg cgtcgagact gacgatcaga tacgtcacga
      841 agaaggccag cagggtgcag gcgccatacc agacgaacaa caactgggtt tcctgaccgg
      901 cgagcaggtt tcgcgacagt agcaccgccc cataaacggc cagcaagctc agcggccaga
      961 cgccgagcat gccgccccac aacaggtcga tgaacagacc gacgaagaac agcaccgccg
     1021 gcgcgatcat cgacgggcgc accagcggcc aggcgaacgc gaggatcatc ggcaggaccg
     1081 gctccggcag tttcagcccg aacaattgga ccggggtggc gaggacaagt gtcgctgcaa
     1141 tgacgatcag cgacggatag acgatccact ccacaggacc gacgacgcgg accgcagcgg
     1201 accgtttcac agcgcgcccc ccggctgagt cggcgcagga acgccggttg gcgtcgcacc
     1261 gggcgccgcc tgagtcggtc ttggcgcgac aggtcgtggt gcagtgggtc tcggtgcggc
     1321 cgcaatggcg gcgggagaag tggctgcagg cggggtcgcg ctggccgccc gcgcgcgctc
     1381 gaccgcgctt gctgcagcgg ccgcgcgtcg cgcagaggcg tcgcctatgg ccgccgcctg
     1441 ggccgagcca ggactcggag cagttcccaa tccggccaga ggcgcggcgt tcagggcctc
     1501 ggggttcacc agttggccaa agtcctggaa cagcatgacc cggacatagt cgatggcgcc
     1561 ccggtcgctg aacagcttga cccgccacga tccgtcgatc cccttggcgg cgacgccgat
     1621 tggtacgccg cgcgggatcc cgccaccgtc tccggagctg aggacgcgat ccccggcctg
     1681 aaggtcgcga gaccgcagga actccaggcg gggattgccg ctgccgtcgc ccgtcagcat
     1741 ggcccgggcg tccgtccggt caatcaggat cggggtgcgg ctggacacat ctgtcagcag
     1801 caggacgcgg cttatcccgc cggtcgtacc tatgatgcgc ccgaccatcc cgtgctcatt
     1861 gatgacggga ttaccgatac gaacgccctt ggacgatccc gcgtcgatca ggcgtgcgtt
     1921 gacgaaaggt ccgcgtgcat ctgagacgga ccttgcggtg accatggcca ccgcgggctc
     1981 cgtccgcagg cccagcatcg cctcatagcg ggcgttgacg ttcttgagcg cgatggcctg
     2041 atcgcgccag ggctgcagat cggcgacctg ggcccgcagg cggcggttct cggagaccgc
     2101 gaagaaatag cccttgatcc cgtcgctcag ggcgcccgtc cagcgcaccg gagccgcaag
     2161 cacgccgttg accgggcccg cgacggagtc gaatccagct ctgacagggg tgtagttgtc
     2221 gtggtcgcgg acctcgcgcc gatcgctgac gatcaggaac agcgatgcaa tcaaggcaac
     2281 gatgaccgcc accgcggcgg tccacgccag cggtaccttg aggttctcga acggtccgtc
     2341 gcgaaacgcc acggcagcct ttcttgtaag gggtcagaat cggtgggacg ccccccaccg
     2401 acagtgcagc ctattgcaaa atacggaccc gttgggaacc gcggaaaacg caggagcgac
     2461 ggggatacgc ggccagaccg gccatcatcc ccgtcagtgt cgagctcaga tcgcctaaag
     2521 cgccgagtcg agcacgccct tcatccagcg cgggtgttcc agaacgcgac cgcagccgat
     2581 ggcgacgcag gacagtggat cgtcggcgac cgagaccggc aggccggtat ggtcgcggat
     2641 ttccgcgtca agaccgcgaa gcagagcccc gccgcccgta agcatgatgc ccttgtcggc
     2701 gatgtcggcg gccagttcag gaggcgtggc ttccagggcg accttcacgg cgtcgatgat
     2761 ctgggcgaca ggttcggcca aggcttcggc cgcctgacgt tccgagatgc ggacctcgcg
     2821 cggcacgccc tgcatcaggt cgcgaccctt gacctcgatg aacaggcctt cgccgtcggc
     2881 gggcatccgc gccgtgccga tgtccttctt gatccgctcg gcggtggtct cgccgatcag
     2941 aaggttatgg ttgcggcgca tgtagctgat gatcgagtcg tccatcttgt cgccgccgac
     3001 gcggaccgac ttggaataga cgatgcccga cagcgacagc acggccactt cggtcgtgcc
     3061 gccgccgatg tcgaccacca tcgaacccgt gggctcgtgg atcggcaggc cggcgccgat
     3121 cgcggcggcc atgggctcgt cgatcagacc gacgcggcga gccgaggcgt tcaggcagct
     3181 gtcattgatg gcgcggcgtt cgacggctgt ggcgcccgac ggcacgcaga cgatgatctt
     3241 ggggttcacg aagccgcgac ggttatgcac cttgcggatg aagtgcttga tcatttcctc
     3301 ggcgacttcg aagtcggcga tgaccccgtc tcgcatcgga cggatggctt ccatgtggcc
     3361 cggcgtgcgg cccagcatct gcttggcctc aatgcccacg gcgtggacga tcttgcggcc
     3421 gccgacgttg cgcagtgcaa cgaccgaagg ctcgttcagg acaatcccct tgcccttcat
     3481 gtaaatgagg gtgttggcgg tgcccagatc catcgcgatg tcgttggaga tcgcgccgaa
     3541 aagatgagaa aacatggggg tcggttaccg tctggttcgg gcc
//
LOCUS       DASVTM010000487        11745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_47354, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000487 DASVTM010000000
VERSION     DASVTM010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47354"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2250)
                     /locus_tag="VF633_13465"
     CDS             complement(<1..2250)
                     /locus_tag="VF633_13465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX8472113.1"
                     /translation="MTDNTESARNEDTAEEAAYGAESIKVLKGLDAVRKRPGMYIGDT
                     DDGSGLHHMVYEVVDNAIDEALAGHADLVEVILNADGSVTVTDNGRGIPTDIHAEEGV
                     SAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLKLVIFRNGKRHEMKFE
                     RGDTVESLKVTGDAPMRDNGKVLSGTQVTFYPSVTTFSHIDFDLKTLEHRLRELAFLN
                     SGVVIKLADHRGVEQIEQLLHYEGGVEAFVRHLDKSKTPILKDVIVIRGKKDNIEIDL
                     ALWWNDSYHETMLCFTNNIPQRDGGTHLSAFRTSLTKVMGAYMESSGALKKEKVAPSG
                     EDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPAVEGLCSEGLSQWFEEHPIEAKL
                     VVAKIIEAASAREAARKARDLTRRKSALDISSLPGKLADCQERDPAKSELFIVEGDSA
                     GGSVKQGRNRENQAVLPLRGKILNVERARFDRMLSSELIGTLILALGTGIGRDDFNAD
                     KLRYHKIILMADADVDGAHIRTLLLTFFYRQMPELITRGHIYIAQPPLYKVSKGKQSR
                     YLKDQSEMDSYLIEEGSSEAELDLANGERRTGHDLQALVREAKTFKSLSDRLSQRAPA
                     FSIEQSALAGLFAEEGDLAAKAAAAATRLDLYAEEGDGAWSGEPGVQGAVVFNRTRRA
                     VKESVVLEEALIRSLDARRLAERALAFDGVFDKPAIYRRKDKSTTIRGPIDLLNAILD
                     AGKKGLAIQR"
     gene            complement(2429..2950)
                     /locus_tag="VF633_13470"
     CDS             complement(2429..2950)
                     /locus_tag="VF633_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX8472114.1"
                     /translation="MTDDRSVLEAPVPPAITRRLEQVLKPIDAAGLTVACAESCTGGL
                     VAAAISDVEGLGHVLDGALVTYSNEAKKRLLGIPADLIEDPGPVSEVVAIRMAEGALT
                     RSAADIALSVTGFAGPAGPDDEEGLVHFAMARTDRDTVHREGHFGEIGRSRVRLACLE
                     VMIDLLEEAGADA"
     gene            complement(3025..4176)
                     /gene="recF"
                     /locus_tag="VF633_13475"
     CDS             complement(3025..4176)
                     /gene="recF"
                     /locus_tag="VF633_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX8472115.1"
                     /translation="MSLTSLTLTDFRSYASATLPVGAGPVVLHGPNGAGKTNLLEALS
                     LFTPGKGLRGATAQEMGRREPGEAGGRAWAVALTLAPLSEDGGDDEEIRLGTGVQTAG
                     AGRRIVRIDGETAAPGRLLDYLRPVWATPEQDRLFSDARADRLKFFDRLVFAANTAHA
                     ATVSAYEKAMRERLRLLTDGVEGREADPLWLDALELRLGDTGARAAMARASALELLQA
                     GIDARGDRPFPQADLGLTGEAETMAAAGADHAAIAGAIREGMLRSRRRDGAAGRSLFG
                     PHRTDLTALHREKNRPAAEGSSGEQKALVLNLILAQVTRLKSEAKDGGGASPVLLLDE
                     APAHLDARRRSALFDEIVALELQAFMTGTEAVLFDDLQGRARFVRVEGG"
     gene            complement(4173..4766)
                     /locus_tag="VF633_13480"
     CDS             complement(4173..4766)
                     /locus_tag="VF633_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472116.1"
                     /translation="MSDERVESLTPDAVQETPSLTVRTDTLKAIGPVLVVWALLFALF
                     ALPLTHTALVAAEGGDSLAAWGRLAATRGFWWVAAPYAAVALLIGYYVWILTRRLLIQ
                     PTILLTPEGFEVTDEAGVRFTAWGDVEAFDIAYDPDNHREHLGWRYRLGAARVSEWDR
                     PARNLDKTFGMGWQGSVPDLRDTFEAWRRRYSAGRID"
     gene            complement(4759..5877)
                     /gene="dnaN"
                     /locus_tag="VF633_13485"
     CDS             complement(4759..5877)
                     /gene="dnaN"
                     /locus_tag="VF633_13485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX8472117.1"
                     /translation="MQLTIERSALLKALGHVQSVVERRNTIPILSNVLLSAGRDSLSF
                     AATDLDMEMVDRADAVVQVEGQITAPAHTLYEIVRKLPDGAEVSLTYNGDDPRLTVSA
                     GRSRFNLPVLPAGDFPVMSTDTSGARFSLMKEDLARLIDKTRFAVSTEETRYYLNGLY
                     LHTVADNGIPLLRAVATDGHRLALAETPAPEGAAGGPGVIVPRKTIDQVRRLLDDGSG
                     PVEVQVSPQKIRFEFGQASLTSKVIDGAFPDYMRVIPKGNDKQADIDNAMFSKAVDRV
                     ATISAEKSRSVKLAFELDRVTLTVRNMEAGQGVEEVEIGYSEEPFEIGFNARYLLDVA
                     GQITGENATFMFADPASPTLVLDPGDPGVQYVLMPLRV"
     gene            complement(6230..7651)
                     /gene="dnaA"
                     /locus_tag="VF633_13490"
     CDS             complement(6230..7651)
                     /gene="dnaA"
                     /locus_tag="VF633_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX8472118.1"
                     /translation="MSMTDPDRIWTEAAGRLRAEIGEGPFSSYIAPSAVRADGSGQLI
                     LVTPTAYARDWVRKNALRRMNELWLGLDGLSRRLEVRCRAEVGSPAPASAVSAPSLVM
                     SGDTAVAAMPVATVAPVADGARAVRAAGLQERLTFDSFVEGQGNAFALAIAKQVASWA
                     DGHFNPVFFCGPYGYGKTHLLNAIAWEAQRLRPEAKVVYLTAERFLSTFVRAMQDRST
                     AAFKESLRSADMLLLDDVQFVGGKASTQEELLSTLTALIEDGKRIVLSADRPPTALSE
                     VEPRLRSHLSAGLTCPVEPADRALKIAVAQNRLAAFARMGVVVGEASSAVLEQLVDRT
                     PGSVRELEGAVTTLAAAAGSRLSSLGADEAAGLLGAALRGGPERRITVDEIQKTVAEH
                     FNMKQVDLLSERRTRAIARPRQIAMYLCKQHTTRSYPDIGRRFGGRDHTTVLHGVRKI
                     EELMPQDDQIARDVEALTRKLRG"
     gene            complement(8199..8468)
                     /gene="rpsT"
                     /locus_tag="VF633_13495"
     CDS             complement(8199..8468)
                     /gene="rpsT"
                     /locus_tag="VF633_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX8472119.1"
                     /translation="MANNPGARKAIRKIAARTEVNKARRTRVRTFLRKFQEAVVAGDA
                     GAAKDAFVTAQSELMRAVTKGVVHKNTGSRKVSRLAAQLKKMPAA"
     gene            8680..9261
                     /locus_tag="VF633_13500"
     CDS             8680..9261
                     /locus_tag="VF633_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8472120.1"
                     /translation="MTSAYRPFAAALSALMLVASPATVLAQTSTAPTPAPTSANDAVA
                     ALLGEQEAPPTPELATPAATANAALEARGETNGPAPDSAQNPVELTITQRLNAEVVRR
                     DEMADQTDRTNQAEYKAEVAEYKEAVADNQAERERIVIDAQINTQARAEYDADQESRY
                     QKAMADWRATNAACERGDAARCRAGSMPARPGT"
     gene            complement(9275..10075)
                     /locus_tag="VF633_13505"
     CDS             complement(9275..10075)
                     /locus_tag="VF633_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8472121.1"
                     /translation="MSDAPADAYDNILIERHADGYAVVTLNRPEALNALNAALFADLA
                     AFLDAVERDDSVRCLILTGSGEKAFAAGADIKEMADQTYAQMYADNYFALGHDRIMRF
                     RKPIIAAVNGFALGGGCELAMLCDFIIASDKAKFGQPEINLGVAPGIGGSQRLTRLVG
                     KAKAMDMVLTARMMDAAEAERAGLASRVFPHDTLMDEVRKIAAKIASQSPLAVMANKE
                     MVNAALETTLTQGVQFERRLFHSLFAFDDQKEGMAAFVEKRKPAFKGT"
     gene            complement(10171..10401)
                     /locus_tag="VF633_13510"
     CDS             complement(10171..10401)
                     /locus_tag="VF633_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8472122.1"
                     /translation="MAPLLKGGPPPIRNRIRELRFHAAEMTQAALAERIGMTRQTIVA
                     IEQGKYSPSLEAAFRIAQVFGVPITDVFEWDG"
     gene            complement(10401..10889)
                     /locus_tag="VF633_13515"
     CDS             complement(10401..10889)
                     /locus_tag="VF633_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472123.1"
                     /translation="MSFREKHLWISIVSGVVVWGWYFWFLIRHVAAGRLVNDHFTGDV
                     SLAFMGCLVLVALIEVVLTFIALATTPKVQRKTKDEREMLAALKASHVALMALIALIF
                     CVSMAAYFAGLIDDAFVGGAAVFSVTGNLMVLFANVLLACLVLAELVRAGVTLMLLRG
                     LR"
     gene            11091..>11745
                     /locus_tag="VF633_13520"
     CDS             11091..>11745
                     /locus_tag="VF633_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472124.1"
                     /translation="MRWMSFAAALAATLVAALTSMNATAMPATPSSDFTPCPDATTGP
                     LAGSLCTVARSPLRHGDPASGDIEVFVRRFPAIGVSRGDVWLVAGGPGEAGAGFYGLI
                     DRFRTAFPEYDLIVPDHRGTGQSSRVCVAEEAADSLGGRGLEGAEWGACFGQLQARSP
                     WVQSFTITNAAHDLGGLIARYSAGRETWVYGVSYGTQLVLRMMTVAPQTSVEGIVLDS
                     "
BASE COUNT         2039 a         3837 c         3881 g         1988 t
ORIGIN      
        1 gcgttggatg gcgaggccct tcttgcccgc atcgaggatg gcgttgagca ggtcgatcgg
       61 accgcgaatg gtcgtcgact tgtccttgcg gcgatagatg gccggcttgt cgaacacgcc
      121 gtcgaacgcc agcgcccgtt cggccagacg acgcgcgtcc agcgagcgga tcagcgcctc
      181 ttccagcacc acggattcct tgaccgcacg acgggtgcgg ttgaacacca ccgcgccttg
      241 cacgcccggt tcgcccgacc aggcgccgtc gccttcttcg gcgtagaggt ccagacgcgt
      301 ggcggcggcc gcagccttgg cggcgagatc gccctcctcc gcgaacagac cggccagcgc
      361 cgattgctcg atggagaagg cgggcgcgcg ttgcgacaga cggtcggaca gcgacttgaa
      421 cgtcttggcc tcgcggacga gcgcctgcag gtcgtgaccc gtgcgccgct cgccgttggc
      481 cagatccagt tcggcctcgg acgagccttc ctcgatcagg tacgagtcca tttcggactg
      541 gtccttgagg tagcgcgact gcttgccctt ggagactttg tagagcggcg gctgggcgat
      601 gtagatgtgg ccgcgcgtga tcagctcggg catctggcga tagaagaagg tcagcagcag
      661 ggtccggatg tgggcgccgt cgacgtcggc gtcggccatc aaaatgatct tgtggtagcg
      721 cagcttgtcg gcgttgaagt cgtcgcggcc gatgccggtg ccgagcgcca ggatcagcgt
      781 cccgatcagc tccgacgaca gcatccggtc gaaccgggcg cgctcgacgt tgaggatctt
      841 gccccgcagc ggcaggaccg cctggttctc gcggttgcgg ccttgtttga ccgagccgcc
      901 ggcggagtcg ccctcgacga tgaacagttc ggacttggcc ggatcgcgtt cctgacagtc
      961 ggccagcttg ccgggcaggc tcgagatatc cagcgccgac ttgcgccggg tcaggtcgcg
     1021 ggccttgcgc gccgcttcac gggccgaggc cgcctcgatg atcttggcga cgaccagctt
     1081 ggcctcgatc ggatgttcct cgaaccactg cgacaggcct tcggagcaca gcccctctac
     1141 cgccgggcgc acttcggacg agaccagctt gtccttggtt tgcgacgaga atttggggtc
     1201 cggaaccttg accgacagga cgcaggtcag gccttcgcgg gcgtcttcac ccgacggcgc
     1261 gaccttttcc ttcttcagcg cgcccgagct ttccatgtag gcgcccatga ccttggtcag
     1321 gctggtgcgg aaggccgaga ggtgggtgcc gccgtcccgc tgggggatgt tgttggtgaa
     1381 gcacagcatc gtctcgtggt aggaatcgtt ccaccataag gcgaggtcga tctcgatatt
     1441 gtccttcttg ccgcgaatga cgatgacgtc cttaaggatc ggcgtcttgg acttgtcgag
     1501 gtggcgcacg aaggcctcga cgccgccttc gtagtgcaga agctgctcga tctgctcgac
     1561 gccgcgatgg tccgccagct tgatgaccac acccgagttc aggaaggcca gctcacgcag
     1621 gcggtgctcg agcgtcttca ggtcgaagtc gatgtggctg aacgtcgtta ccgacggata
     1681 gaaggtgacc tgggtgccgc tgaggacctt gccattgtcg cgcatcggcg cgtcgccggt
     1741 gaccttgagg ctctcgaccg tgtcgccacg ctcgaacttc atctcatggc gcttgccgtt
     1801 gcggaagatg accagcttga gccagtcgct cagcgcgttg acgaccgaga cgcccacgcc
     1861 gtgcaggccg cccgagacct tgtaggaatt ctggtcgaac ttaccgcctg cgtgcagctg
     1921 ggtcatgatg acctcggccg ccgagacgcc ctcttcggcg tggatgtcgg tggggatgcc
     1981 acgaccgttg tcggtgacgg tgaccgagcc gtcggcgttc aggatgacct cgaccagatc
     2041 ggcgtggccg gcgagggcct catcgatggc gttgtcgacg acttcgtaca ccatgtggtg
     2101 caggccggag ccgtcgtcgg tgtcgccgat atacatgccg ggacgcttgc gaacagcgtc
     2161 caggcctttg agaaccttga tggattccgc gccgtaggcg gcttcttcag ccgtgtcttc
     2221 gttgcgggca gattcggtgt tgtcggtcat tcaatcgtcc ggagacgcgg gggtcgatgc
     2281 ggatggcgaa gagcgatccg gcccgctgaa aaatgcacaa ggatcgcgac cgattcaggc
     2341 cgcgtcgatg ctgtgtttat acaggattcc ggacgggaaa agcgagggaa agcggaggcg
     2401 tagtacctga ctgccgcaag ccgagcggtc aggcgtccgc gcctgcctct tcgagcaagt
     2461 cgatcataac ttccagacag gcgagccgca cccgggagcg accgatttct ccgaaatggc
     2521 cctcgcggtg cacggtatcc cggtcggtcc gcgccatggc aaaatggacc agcccctcct
     2581 cgtcatcggg accggcgggg cctgcaaacc ccgtcaccga cagcgcaatg tcggccgcag
     2641 agcgggtcag ggcgccctcg gccatccgga tcgccacgac ttcggacacc ggccccggat
     2701 cctcgatcag atcggcgggg atgcccaaca gccgcttctt ggcctcgttc gaataggtga
     2761 ccagagcgcc gtccagcaca tgacccagac cttcgacgtc ggagatggcg gctgcgacaa
     2821 gtccgccggt gcagctctcg gcgcaggcga cggtcaatcc tgcggcatcg atcggcttga
     2881 gcacctgttc cagccgccgc gtgatcgcgg gcggcaccgg agcctcgaga acgcttctgt
     2941 cgtcggtcat gcgaaagtaa cgaggcgccg ggcgggaccg ttcctacgcc gggctcccga
     3001 cattggtcag tccgaggcct ccagtcagcc gccctcgacc ctgacgaacc gcgcgcggcc
     3061 ttgcaggtcg tcgaacagca cggcttcggt gccggtcatg aaagcctgaa gctcaagcgc
     3121 cacgatctca tcgaacaggg cggagcggcg gcgggcgtcg aggtgggcgg gggcctcgtc
     3181 cagcagcagg acgggggacg cgcctccccc gtctttggct tccgatttca gccgggtcac
     3241 ctgggcgagg atcaggttca ggaccagcgc cttctgctcg cccgaggagc cctcggcggc
     3301 ggggcggttc ttttcacggt gcagggcggt caggtcggtt cgatgaggac cgaacaggga
     3361 gcggcctgcc gcgccgtcgc gtcgccttga gcgcaacatc ccttcgcgga tcgcaccggc
     3421 gatggccgcg tgatcggctc cggcggcggc catggtctcg gcctcgccgg tcaggccgag
     3481 gtcggcctgt gggaatggcc ggtcgccccg ggcatcgata cccgcctgta gcagttccag
     3541 agcagaggcg cgggccatcg cagcgcgcgc tccggtgtcg cccagccgca gttccagcgc
     3601 gtcgagccag agcggatcgg cctccctgcc ctcaaccccg tcggtcagca accgcagccg
     3661 ctcgcgcatg gccttctcat aggccgagac ggtggccgcg tgcgcagtgt tggccgcgaa
     3721 caccagccgg tcgaaaaact tcagtcggtc cgcccgggcg tccgagaaca atcggtcctg
     3781 ctcgggcgtc gcccagacgg gccgcaggta gtcgagcagc cgccccggcg ccgccgtctc
     3841 gccgtcgatg cggacgatgc gccgccccgc gcccgccgtc tgaacgcccg tgccgaggcg
     3901 gatctcttca tcgtctccgc cgtcttccga aagaggggcg agcgtcaacg ccacagccca
     3961 cgcccgaccg cctgcctcgc ccggctcccg ccgccccatc tcctgggccg tcgccccacg
     4021 cagacccttg ccgggcgtga acaggctcaa cgcctccagc agattggtct tccccgcccc
     4081 gttcggccca tgcagcacca cagggcccgc gcccacaggc agggttgcgg acgcgtagga
     4141 ccggaaatcg gtaagggtaa gggaagtcag gctcaatcta tccgcccggc gctataccgg
     4201 cgacgccacg cctcgaatgt atcgcgcaaa tccggcacgg aaccttgcca gcccatgccg
     4261 aatgtcttgt cgaggttccg ggcaggcctg tcccactcgc tcacccgggc ggcgccaagt
     4321 ctgtaacgcc agccgaggtg ctcgcggtga ttgtcaggat cataggcgat gtcgaacgcc
     4381 tcgacatcgc cccatgccgt aaagcggacg ccagcctcgt ccgtaacctc gaacccttca
     4441 ggcgtcagca aaatggtcgg ctggatgagc aggcgtcgcg tcagaatcca gacatagtat
     4501 ccgatcaaca gcgccacagc ggcatagggc gccgccaccc accagaagcc ccgggtcgca
     4561 gccagccgac cccatgcggc cagcgagtcg ccgccctccg ccgccacgag agccgtgtgc
     4621 gtaagtggca aggcgaacag ggcgaagaga agagcccaga cgaccagaac cggccctatt
     4681 gctttcagcg tatccgtccg caccgtcagc gacggagttt cctgtaccgc gtctggagtt
     4741 aggctctcga cgcgttcgtc agacacgcag cggcatcagc acgtactgaa cgccgggatc
     4801 gcccggatcg agaacgagcg tcggcgaggc ggggtcggcg aacatgaagg tggcgttctc
     4861 accggtgatc tgaccggcga cgtccagcag atagcgggcg ttgaagccga tttcgaaggg
     4921 ctcttcggag tagccgatct cgacctcctc gacgccctgc ccggcctcca tgttgcgcac
     4981 cgtcagggtg acgcggtcga gttcaaacgc cagcttcacc gagcggctct tttcggccga
     5041 gatggtggcg acgcggtcga cggccttgga gaacatggcg ttgtcgatgt cggcctgttt
     5101 gtcgttcccc ttggggatga cgcgcatata gtcggggaag gcgccgtcga tgaccttgga
     5161 ggtcaaggag gcctggccga actcgaaacg gatcttctgg ggcgagacct gaacctcgac
     5221 ggggccggag ccgtcgtcga gcaggcggcg gacctgatcg atggtcttgc ggggtacgat
     5281 tacgcccgga ccaccggcgg cgccttcggg agccggcgtc tccgccagag cgaggcggtg
     5341 accgtcggta gccacggcgc gcagcagcgg gatgccgtta tcggccaccg tgtgcagata
     5401 gaggccgttc agatagtagc gggtttcctc ggtcgagacc gcgaaccgcg tcttgtcgat
     5461 caggcgggcg aggtcttcct tcatcaggct gaaccgggcg ccggaggtat ccgtcgacat
     5521 caccgggaag tcgccggcgg gcaggaccgg caggttaaag cgcgaacgtc cggccgagac
     5581 cgtcagccgg ggatcgtcgc cgttataggt cagcgagacc tcggcgccgt cgggcagttt
     5641 gcgcacgatc tcgtacaggg tgtgggccgg ggcggtgatc tggccctcga cctgaaccac
     5701 ggcgtcggcg cggtcgacca tttccatgtc gaggtcggtc gcggcgaacg acaggctgtc
     5761 gcggcccgcg ctcaacagga cgttggacag gatcggaatg gtgttgcggc gctcgaccac
     5821 gctctgcaca tgcccgaggg ccttcagaag cgcggatcgt tcgatggtca gctgcatgtc
     5881 gtctcaaagc ccgggcgcaa cgcggaatcg cagcgtcgct tgtgtattgg ctttggacac
     5941 tagcggattg gcgcggcgga gcaactagca ggcagcatgg tccacaactc acctctccat
     6001 tcgcgaaggc gaatggagag gtgaggtcgg cgcgcttgcg acgagcaggt gagggaaacg
     6061 cggaattagg aaaggggtga ccggccagca ccgtgtcgcc ccctcctgat cgcccaccga
     6121 tcgcaccgcg atcggcccct ccaccgcttc gcggtccccc tccccatcag ggatggggag
     6181 gatatgcgat ctgtcctccc cacgcgcctt cgcgcgcgga gaggtgaact cagcctctca
     6241 gcttgcgcgt caacgcctcg acgtcgcggg cgatctggtc gtcctgcggc atcagttcct
     6301 cgatcttgcg cacgccgtgc aggaccgtgg tgtggtcgcg accgccgaag cgacgaccga
     6361 tgtcgggata ggagcgggtc gtgtgctgct tgcacaggta catggcgatc tggcgcggac
     6421 gggcgatggc gcgggtgcga cgctcggaca gcagatcgac ctgcttcatg ttgaaatgct
     6481 cggcgacggt cttctggatc tcgtcgacgg tgatgcgacg ctcgggaccg ccgcgcaggg
     6541 cggcgcccag cagaccggcg gcttcatccg cgcccaggct cgacagacgc gacccggcgg
     6601 cggcggccag ggtggtgacg gcgccctcaa gctcacggac cgagcccggc gtgcgatcga
     6661 ccagttgttc cagcacggcg ctggacgctt ccccgacgac cacgcccatg cgggcgaagg
     6721 cggccaggcg gttctgggcg acggcgatct tgagcgcgcg gtcggcgggc tcgaccgggc
     6781 aggtcaggcc ggcggacagg tggctgcgca ggcgcggctc gacctcggac agggcggtgg
     6841 gcggacggtc cgccgacagc acgatccgct tgccgtcctc gatcagggcg gtgagtgtcg
     6901 agagcagttc ttcctgggtc gaggccttgc cgcccacgaa ctggacgtcg tccagcaaca
     6961 gcatgtcggc cgagcgcagg ctttccttga aggcggcggt cgagcggtcc tgcatggcgc
     7021 gcacgaaggt cgacaggaag cgttcggcgg tcagatagac caccttggcc tcgggccgca
     7081 gacgctgggc ctcccaggcg atggcgttga gcaggtgggt cttgccgtag ccgtaggggc
     7141 cgcaaaagaa gaccggattg aaatggccgt cagcccagct ggcgacctgt ttggcgatgg
     7201 cgagggcgaa ggcgttgccc tggccttcca cgaagctgtc gaaggtcagg cgttcctgca
     7261 gaccggcggc gcgcacggcg cgggcgccgt cggcgacagg agcgacggtg gcgaccggca
     7321 tggcggcgac agcggtgtca ccggacatca ccagcgacgg cgccgacacg gcggaggcgg
     7381 gagcgggcga accgacctcg gccctacaac gaacttccag acggcgcgac aggccatcga
     7441 ggcccagcca cagctcgttc atgcgacgca gggcgttctt gcgcacccag tcacgggcat
     7501 aggccgtcgg ggtcaccagg atcaactgac cggagccgtc cgcccgcacc gccgaggggg
     7561 cgatatagga actgaaaggg ccttcgccga tttccgcgcg aagacgaccc gccgcctccg
     7621 tccagatgcg atccgggtcc gtcatgctca tcactgccaa gccccccatc tttagtcacc
     7681 gcccttgaaa tcacagccgg acgtcaagct gacgttcggc cacgcaccgt catgcccaca
     7741 ggggatcagc cagccgcagg gacgcgttct cctgccccgc gaagcatgcg cttcacgggc
     7801 cgggtgcggc ctctaggttt gacgtctccc gccgactgga accctgttcc cgccgtcttc
     7861 atccgtgtgg ggatggaagt gcgttagagc ccaaaactta cgcttggcgg gccttgggtc
     7921 aacgctgatt cgagtcacga atcgcagtta aatagtttga ctccgctaaa cccaaatcct
     7981 gcatggggaa atgtggattt caccccacac gcgagctatt tatcgcaaat gttaagttag
     8041 aggcagacgt gcaaacggcg aggaagcaga tgcttcctcg ccgttgaaat cactcataat
     8101 tcgcatgacg gttcgaaaaa atccgatccg tgtagccgca gactgaaagc ttcgccttgg
     8161 agggtgcgcc tgaacctaag gttacccctt caggggtatc aggcggccgg cattttcttg
     8221 agctgggcag ccagtcggct gaccttgcgc gagcccgtgt tcttgtggac cacgcccttg
     8281 gtgacggcac gcatcagctc ggattgagcc gtgacgaatg cgtccttggc ggcgccggcg
     8341 tcgccagcga cgacagcttc ctggaattta cgcaggaagg tccggacgcg cgtgcggcgc
     8401 gccttgttca cttcggtgcg ggcggcgatc ttacggatcg ccttgcgggc gccggggttg
     8461 ttggccatcg ataaacctat gagacggaaa acgccgcgga gcacagtgtc cacggcgcag
     8521 aaatctgaag aggcgcggct atagctgaac ccacggcagg ggtcaacgcg agtccgtcgc
     8581 ttctcgctct attgccgact accgcgtccg ggacgcctgc gggtgcatgg gtccaatcgc
     8641 cccggcacgc gttatataca gctgagcaag gagctttcga tgacatccgc ttaccgtccg
     8701 ttcgccgccg ccctgtccgc cctgatgctg gtcgcgtcgc cggcgaccgt gctggcgcag
     8761 acatccaccg cgccaacacc cgccccaaca tctgccaatg acgccgtggc cgcgctgctg
     8821 ggcgaacagg aggctccgcc cacgcctgag ctggcgacac ccgccgcgac ggccaatgct
     8881 gcgctggagg ccagaggcga gaccaacggg cccgctccgg attcggcgca aaacccggtc
     8941 gagttgacga tcacgcagcg gctgaacgcc gaggtcgtac ggcgcgacga aatggccgac
     9001 cagacggacc gcaccaacca ggccgagtac aaagcagagg tcgccgagta caaggaagcg
     9061 gtggccgaca atcaggccga gcgcgaacgc atcgtcatcg acgcccagat caacacccag
     9121 gcgcgcgccg aatacgacgc cgatcaggaa tctcgctacc agaaggccat ggcggactgg
     9181 agggctacca acgccgcctg cgagcggggc gacgccgcgc gatgccgtgc cggatccatg
     9241 cctgcgcgtc cgggaaccta ggcgccccct gctatcaggt gcccttgaag gcgggtttcc
     9301 gcttctcgac aaaggcagcc atgccctctt tctgatcgtc gaaggcgaac agggagtgga
     9361 acaggcggcg ttcgaactgc accccttgcg tcagcgtcgt ctcgagggcg gcgttgacca
     9421 tctccttgtt ggccatcacg gccagcgggc tttgcgaggc gatcttcgca gcgatcttgc
     9481 ggacctcgtc catcagggtg tcgtgcggga acacgcgtga cgcgagaccg gcgcgttcgg
     9541 cctcggcggc gtccatcatc cgcgcggtca ggaccatgtc catggccttg gccttgccga
     9601 ccagccgcgt cagccgctgc gagccgccga tgccgggcgc gacgccgagg ttgatctcgg
     9661 gctggccgaa cttcgccttg tccgaagcga tgatgaagtc gcacagcatg gccagctcgc
     9721 agccgccgcc gagcgcaaag ccgttgaccg ccgcgatgat cggcttcctg aaccgcatga
     9781 tccggtcgtg gccgagagcg aaatagttgt cggcgtacat ctgcgcatag gtctggtccg
     9841 ccatctcctt gatgtcggcc ccggcggcga aggccttctc acccgagccc gtcaggatca
     9901 ggcagcgcac gctgtcatcg cgctcgaccg cgtcgagaaa cgccgccaga tcggcgaaca
     9961 gcgccgcgtt caacgcattc agcgcctcgg gccggttcaa cgtcaccacc gcatagccgt
    10021 cagcgtggcg ttcgatcagg atgttgtcat aggcgtcggc gggggcatcg gacatgggag
    10081 cgctccttgg atgaccgttc catatccatc ccgccatccc cgcgaaagcc ttgcttgctt
    10141 ccctctcccg gcgggagaga gtggagatca ctacccgtcc cactcgaaaa cgtcggtgat
    10201 cggcacgccg aacacctggg cgatgcggaa ggcggcctcg aggctgggtg agtatttgcc
    10261 ctgctcgatg gcgacgatgg tctggcgcgt catgccgatc cgctcggcca gcgccgcctg
    10321 ggtcatctct gcggcatgga agcgcagctc gcggatgcgg ttcctgatcg gcggagggcc
    10381 gcctttcagt agcggagcca tcagcgcagg ccgcgcaaca gcatcagcgt cacccccgcc
    10441 cgtaccagct cggccagcac cagacaggcg agcagcacat tggcgaacag caccatcagg
    10501 ttgccggtga ccgaaaagac cgccgctccg ccgacaaaag cgtcgtcaat cagcccggcg
    10561 aaataggccg ccatcgacac gcagaagatc agggcgatca gcgccatcaa ggcgacgtgg
    10621 ctggccttga gggcggccag catctcgcgc tcgtccttcg tcttgcgctg gaccttgggt
    10681 gtcgtcgcca aggcgatgaa ggtcagcacc acctcgatca acgccaccag caccagacac
    10741 cccatgaagg ccagactgac gtcgccggtg aagtggtcgt tgaccaaccg ccccgctgcc
    10801 acatgccgga tcaggaacca gaaataccag ccccagacca ccacgccgga gacgatgctg
    10861 atccaaaggt gcttttcacg aaacgacatg gagaggtcct cgctgagcct tcggtagaga
    10921 cattgatgtc tcacccagac gacacgatgt ccagtatttt ttacttttag tccgatttaa
    10981 gtgacacgcc ccggaaggtt cgaaggagtg gcgggacatc attagagaag cttaacgtgc
    11041 actcgcctcc gatcccggcg atgtcactct caagagcagg gaaatgttgc atgcgctgga
    11101 tgagtttcgc agcggcgctg gccgccacgc tggtagccgc gttgacctcc atgaacgcca
    11161 cagccatgcc ggctacgcca tcatctgatt tcactccttg tccggacgcg acgaccggtc
    11221 ccctcgcagg gagtctctgc accgtagcca ggtctccgct tcgccacggc gaccccgcat
    11281 ccggagacat cgaggtgttc gttcggaggt tccccgcgat cggggtcagt cgtggcgacg
    11341 tctggcttgt cgcaggcggt ccgggcgagg cgggggccgg cttctacggc ctcatcgacc
    11401 ggttcagaac ggcctttccg gagtacgacc tgatcgtgcc ggatcaccgc ggcacaggac
    11461 aatccagccg ggtctgcgtc gccgaagaag cggcggactc gctcggcggc cgcggcctgg
    11521 aaggcgcgga atggggcgcc tgtttcggcc agcttcaggc gcggtcacca tgggtccagt
    11581 ccttcaccat caccaacgcc gcgcacgatc tgggcggcct catcgcgcgc tactccgccg
    11641 gacgcgagac ctgggtctat ggtgtatcct acggcaccca actggtgttg cgcatgatga
    11701 cagtcgcccc gcaaacgtcc gttgaaggca tcgttctgga ttcac
//
LOCUS       DASVTM010000488         5432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_364473,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000488 DASVTM010000000
VERSION     DASVTM010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364473"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF633_13525"
     CDS             <1..342
                     /locus_tag="VF633_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472125.1"
                     /translation="GAEATVWRDDLDRPLVEATPLGSGRLMRFTRPLTPAVLPELLQA
                     DFPIQLRAAIDDPLPAPARVAATGYSPLTGGSAFAPGPQDLRPWLAILIGLLLLCERW
                     LATRRSRGVNP"
     gene            339..2456
                     /locus_tag="VF633_13530"
     CDS             339..2456
                     /locus_tag="VF633_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HEX8472126.1"
                     /translation="MSAKTLLATWTAPARRRSLFNDLLLAAPVVVIATSLASVTGGAV
                     VGALVLALGAAIAGLLAVFRQARFDRHWLTHRLNAARLDMEDSAELLFADPQDLRALQ
                     RLQRSRLFDRLGAGSPNALAGNWSRRPILILWAAALIVVAAVVLWPDAEHRRVTLSPS
                     AEGPVAPGIPRLVGQNLRIYPPAYTGLPMRDEARLDARAPQGSRLEWTLRFDPQASVP
                     AMLVLGGAPLALSRSGDDWIASRTLDASFLYRVDPAQGRAGTPPLHRIDAIADTPPQV
                     KVLSPAESLTLVAAGQRAWSPVFAVTDDYGVAPTARLRITMAIGEGENVTFSEREIAV
                     VGSGPARDRRFSPRLDFTALGFGRGSDMVVQLVVSDKRAPGPQVVRAPSLILRWPSAN
                     KAQSSGLEGMVNTTLPAYFRSQRQIIIDAEGLIAQRRRLDPDRYLTRSTVIGGDQEIL
                     RARYSQFLGGEQEEAPELPTSDGAAHSDGDGHDHGAPATRSPGFGGPEDVLAEFGHPH
                     DDSPASSLDPQTRAILKQAVDEMWLSERELKQGHPELALPHANKALDFIKEVQRATRI
                     FLNRVGPELPPIDPARRMTGKREGIAGRALPVAPVAGTDSPAAGAWRALGQGPEAVRG
                     PIALGELERWIGANSGRLPDALSLSGALDAVRRDPDCGECRQRLRALLWTAMERPPAN
                     LDRRRPADPTGARYLGALGGGDR"
     gene            2453..4321
                     /locus_tag="VF633_13535"
     CDS             2453..4321
                     /locus_tag="VF633_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472127.1"
                     /translation="MTAKMILAGILAVAVCAAWIRLILWRRAAPALAPPVWRFATLMA
                     LQPVWAALLFLGLFPPGRPVAGGVLTIVTANAARPVTGPHPIAMPEFSATGIERAPDL
                     GTALRRYPDTRGIRVLGDGLTARDRDAARDLTIDFTPGAPRPGLTSLTPPGPVAPGMR
                     FQSGGQVAGLRDAVVDLLDPAGRVTDTQTVDADGRFMVSGTTRAAGLATFVVRVRDRS
                     GRVAEQADLPVRIQADSAPRLLILAGAPGPEVKYLRRWATDAGFSVNTQMSAGAGIQL
                     GDPAIAVNGATLRQFDVAVIDDRSWSGLGGAREAVLAAVRGGMGLVMRTSGPLDGSAR
                     ASWRSLGFSLNGPDVLGPVALPPVADAAVARTRSGIGSPDAPGDLAAVEPIIPELNRL
                     AATPGGSETVPLVRDAGGAILSVWRAMGLGRVAVFTGVDSYGLTLTGHGGLYGDWWSE
                     MLSTVARPTAGPPVIPGSAWVGDRVSLCGLGSQVRVEQPDGAIRALIADPVAAGCAGF
                     WPVAPGWHLLRMGGPDVRPNGVANGREQVSPFFVQPADKLVGIRSARDRDATLMLAGS
                     APPTVLTGPARTQPGPSWPWLLAWLLVGTILWWFERSSLGRTAPKKRPAHADNNQT"
     gene            complement(4325..>5432)
                     /locus_tag="VF633_13540"
     CDS             complement(4325..>5432)
                     /locus_tag="VF633_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8472128.1"
                     /translation="SAGASFVASRMFLVNEQKYFASTDGSYIDQDVHRIWAPFTVTAV
                     DKATGKFRTRDGLSSPMGLGYEYLEGAAADKTALPGGLIAYDKSYDMREDAVAATAQA
                     KEKLTAASVQPGKYDLVLDPNHLGLTIHESVGHPLELDRVLGYEANYAGTSFATLDKR
                     EARFRYGSEVVNIVADRTQVGSLGAVGYDDEGVASRTWDLIRDGVLVDYQATRDQAHI
                     LGKTESDGCSYADSWSTVQFQRMPNVSLQPGKVKMSASDMIADVENGIYILGRGSFSI
                     DQQRYNAQFGGQLFYEIKNGQITRPLADVAYQMRTPEFWASCAATCDESDYRMFGSFF
                     DGKGQPSQVSAVSHGSATTRFNGINVINTARSLG"
BASE COUNT          765 a         1869 c         1847 g          951 t
ORIGIN      
        1 ggcgcggaag ctacggtttg gcgtgacgat ctcgaccggc cgctggtcga ggcgactcca
       61 ctaggctctg gccgtctgat gaggttcacc cgacctctga ccccggcggt gttgcccgaa
      121 ctgttgcagg ccgacttccc catccagctc cgcgcggcaa tcgacgatcc tctaccggct
      181 ccagcccgtg tggcggctac cggctattcg cccctgaccg gtggcagcgc cttcgccccc
      241 gggccgcagg atttgcgacc gtggctggcg atcctcatcg gcctcctgtt gctgtgcgaa
      301 cgctggctgg ccacgcgtcg ttcgcgcggg gtgaacccgt gagcgccaaa acgcttctcg
      361 cgacctggac cgctcctgcg cgccgacgca gcctgtttaa cgaccttcta ctggccgcac
      421 ccgtcgtggt catcgccacg tccctggctt cggtgacggg cggcgcagtc gtcggcgcgc
      481 tggtcctggc gctcggcgcc gccatcgcgg gtctgctcgc tgtattcaga caggcgcgct
      541 tcgacagaca ttggcttacg catcgtctca acgccgcgcg gctcgatatg gaagatagcg
      601 ccgagctgct gttcgccgat cctcaggatc tccgcgccct gcaacggctg cagcggtcgc
      661 ggctgttcga tcggctgggc gcaggctcgc cgaacgcgct cgcggggaat tggtcgcgtc
      721 gccccatcct gatcctttgg gctgcggcgc tgatcgtcgt cgcagcggtg gttctctggc
      781 cggatgcgga gcatcgccgg gtcaccctgt cgcctagcgc cgaggggccg gtagcgcccg
      841 gcatcccgcg tctggtcggc caaaacctgc gcatctatcc gcccgcctat accggcctgc
      901 cgatgcggga tgaggccagg ctcgacgccc gcgcgcctca agggtcacgt ctggaatgga
      961 cccttcgctt cgatccgcag gcgagcgttc cggccatgct cgtactcggc ggtgcaccat
     1021 tggccttaag ccggtcgggc gacgactgga tcgccagccg cacgctggac gcctccttcc
     1081 tctaccgcgt cgatccggct cagggccgcg ccggtacgcc gcccttgcac cgcatcgacg
     1141 ccatcgccga cacgccgccg caggtgaagg tcctgtcgcc cgccgagagc cttacactgg
     1201 tcgcagccgg tcagcgcgcg tggagccccg tcttcgccgt cacggacgac tatggtgtcg
     1261 cccccaccgc ccgcctccgc atcactatgg ccatcggcga aggcgagaac gtcaccttct
     1321 cggaacgcga gatcgccgtc gtcggcagcg gcccggcgcg cgaccggcgc ttctcgcccc
     1381 ggctcgattt cacggccctc ggcttcggtc ggggcagcga catggtggtg caactggtcg
     1441 tcagcgataa gcgcgccccg ggtccgcagg tggtgcgcgc accgagtctg atcctgcgct
     1501 ggccctcggc gaacaaggcc caaagctcgg gtctggaagg gatggtgaac actacattgc
     1561 cggcctattt ccgcagccag cgccagatca tcatcgacgc cgagggcctg atcgcccagc
     1621 gtcgacgcct cgacccggat cgctatctga cgcgctcgac ggtcatcggc ggggatcagg
     1681 agatcctgcg tgcccgctac agccagtttc tcggcggcga gcaggaggag gcgcccgaac
     1741 tgccgaccag cgacggcgcc gcccactcgg acggggacgg ccacgatcac ggtgcgcctg
     1801 cgacccgcag cccaggcttc ggcggtcccg aggacgtgct ggccgagttc ggccatccgc
     1861 acgacgattc tccggcgtcc agccttgatc cccagacgcg tgcgatcctg aagcaggctg
     1921 tggacgagat gtggctgtcg gagcgggagc tgaaacaagg ccatccggag ctcgccctgc
     1981 cgcacgccaa caaggcgctg gacttcatca aggaggtgca gcgcgcgacc cgcatcttcc
     2041 tgaaccgcgt cgggcccgag ctgccgccca tcgaccccgc gcgacgcatg acgggcaagc
     2101 gtgaaggcat cgccggccgg gccctgcctg tggccccggt cgccggtaca gatagcccgg
     2161 cggccggcgc ctggcgcgcc ttggggcagg gacctgaggc ggtgcgaggg cccatcgctc
     2221 tcggcgaact ggagcgctgg atcggcgcca actcgggacg tctgcccgat gccctgtcgc
     2281 tatcgggggc gctcgacgcc gtgcgccgcg atccggactg cggcgagtgt cgtcagcgtc
     2341 tgcgcgccct gttgtggacc gcgatggagc ggccacccgc caatctcgac cgccgccggc
     2401 cggcggaccc gaccggggcc cgctatctcg gcgcgctggg aggtggcgac cgatgacggc
     2461 gaagatgatc cttgccggca tactggcggt cgcggtctgc gcggcgtgga tccggctgat
     2521 cctgtggcgt cgcgccgccc cggccctggc gccgcccgtc tggcggttcg cgaccttgat
     2581 ggcccttcaa ccggtctggg cggcgttgtt gttcctcggc ctgtttccgc cgggccgccc
     2641 ggtcgcgggc ggcgtcctga ccattgtgac cgccaatgcg gccagaccgg tgacgggccc
     2701 gcatccgatc gccatgccag agttttcggc gaccgggatc gaacgtgctc ctgacctggg
     2761 aaccgcgctt cggcgctatc cggacacgcg gggtatccgc gtgctgggcg acggtctgac
     2821 cgccagggac cgcgacgcgg cgcgcgatct caccatcgac ttcacgcccg gagcgcctcg
     2881 cccggggctg acctccctga ccccgcccgg gccggtcgcg cccggcatgc ggtttcagtc
     2941 cggtggccag gtggccggtc tgcgcgacgc cgtcgtcgat ctgctcgatc ccgcaggacg
     3001 ggtcaccgat acgcagacgg tcgatgcaga cggccggttc atggtctcag gcacgacgcg
     3061 tgccgcgggg ctggctacct tcgtcgtacg tgtgcgcgac cggtcgggac gtgtcgcgga
     3121 gcaggcggat ctgccggtcc ggattcaggc tgattccgcg ccccgtcttt tgatcctcgc
     3181 gggcgctccg gggccagagg tcaaatatct gcgtcgctgg gcgaccgacg ccggcttctc
     3241 ggtcaacacc cagatgagcg ctggtgccgg gatccagctc ggcgaccccg ccatcgccgt
     3301 gaacggcgcg acactgcgtc agttcgatgt cgccgtgatc gacgaccgca gctggtccgg
     3361 actgggtggg gcgcgcgagg cggtgctcgc cgcggttcgc ggcggaatgg gtctggtgat
     3421 gcgcacgagc gggccgttgg atggttccgc gcgcgcttcg tggcgatcgc tcggcttctc
     3481 cctgaacggc cccgacgtcc tgggtccggt cgccttgccg cccgtcgcag atgccgccgt
     3541 cgcgcggacc cgttccggca tcggcagtcc ggatgcgccg ggcgatctgg ctgcggtcga
     3601 gccgatcatt cccgagctca atcgcctggc ggctacgccc gggggaagcg agacagttcc
     3661 gctggttcgt gatgctggcg gggcgatact gtcggtctgg cgcgcgatgg ggctcggccg
     3721 ggtcgcggtg ttcaccggtg tcgacagcta tggtctcacc ctgacaggcc acggtggcct
     3781 ttacggggat tggtggagcg aaatgctatc caccgtggcc cgcccgacgg cgggccctcc
     3841 ggtcatcccg ggctccgcgt gggtcggtga ccgggtctcc ttgtgcggcc tcggtagcca
     3901 ggttcgagtc gagcagcccg acggcgccat cagggccctg atcgccgatc ccgttgcggc
     3961 cggttgcgcc gggttctggc cggtcgcgcc cggctggcat cttctacgca tgggaggacc
     4021 agacgtccgc ccgaacgggg tggctaacgg tcgcgagcag gtctcgccct tctttgtcca
     4081 gcctgctgac aagctggtcg gcatccgctc cgcccgggac cgtgatgcga ccttgatgct
     4141 agcggggtcc gcgccgccca ccgtcttgac cgggccagcc cggacgcagc caggcccttc
     4201 ttggccatgg ctgcttgcct ggcttctggt cggaaccata ctgtggtggt tcgaacgctc
     4261 aagccttggc cggaccgccc ctaagaagcg accggcccac gcggacaaca accagacctg
     4321 acaatcagcc cagagagcgg gcggtgttga tgacgttgat gccgttgaag cgggtcgtcg
     4381 ccgacccgtg gctcaccgcc gaaacctggc tcggctggcc tttaccgtcg aagaaggagc
     4441 cgaacatgcg gtagtcgctc tcgtcgcagg tggcggcgca cgacgcccag aactcgggcg
     4501 tgcgcatctg gtaagcgacg tcggccaagg ggcgcgtaat ctggccgttc ttgatttcgt
     4561 agaacagctg accgccgaac tgggcattgt agcgttgctg gtcgatggag aaagagccgc
     4621 gaccgagtat gtagatcccg ttctctacgt cagcaatcat gtcgctcgcg ctcatcttca
     4681 ccttgccagg ctggagcgaa acattgggca tccgctggaa ctgcaccgtc gaccagctgt
     4741 cggcgtagga acagccgtcc gattccgtct tgccgagaat atgcgcctga tcgcgggtag
     4801 cctgatagtc gacgaggacg ccgtcgcgga taagatccca cgtcctcgac gccacccctt
     4861 cgtcgtcata gcccacagcg ccgaggctgc cgacctgggt tctgtcggcg acgatgttga
     4921 cgacctcgct gccgtacctg aaccgggctt cgcgcttgtc gagcgtggcg aagctcgtgc
     4981 cggcgtagtt ggcctcgtag ccgagaaccc ggtcgagctc gagcgggtga ccgactgact
     5041 cgtgaatcgt caggcctaaa tggttggggt cgagcaccag atcgtacttg cccggctgga
     5101 ctgaagcagc ggtcagtttc tccttggcct gagcggtcgc agcgactgca tcctcgcgca
     5161 tgtcatagga tttatcatag gcgatgagcc cgcccggcag ggccgtcttg tcggcggcgg
     5221 ccccttccag atattcgtag cctagcccca tgggggacga gaggccgtcg cgcgtgcgga
     5281 acttgccggt cgccttgtca acggcagtga cggtgaacgg cgcccagatg cgatggacgt
     5341 cctgatcgat gtacgatccg tcggtgctgg cgaagtattt ctgctcattg accaggaaca
     5401 tgcgtgaggc gacgaaactg gctccggcgg ac
//
LOCUS       DASVTM010000489         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_313596,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000489 DASVTM010000000
VERSION     DASVTM010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313596"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VF633_13545"
     CDS             <1..407
                     /locus_tag="VF633_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX8472129.1"
                     /translation="PFESPLMGLKNVILTPHIGGSTEEAQEAIAEFAAERLLGYLNRG
                     DTTFSVNLPNVQLSEVSGGHRILHIHRNQPGVMAELNRELASAGLNILGQHLKTDERT
                     GYVITDVDRDYDPEALKTLKGVAGTLRFRLLH"
     gene            505..>1861
                     /locus_tag="VF633_13550"
     CDS             505..>1861
                     /locus_tag="VF633_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8472130.1"
                     /translation="MSGFHRGTALAVLAAAATLAAASGASAQTYNRLVVFGDSLSDNG
                     NLYAATGGLQPTSPPYFQGRFSSGPTFVELLGFTLGRSVTGAPVTGSIDYAFGGSRTD
                     SQAFPPGMRLQLQQYTAAGGKFGASDLVSILGGANNIFQGLPAAGASANPTGSISPVA
                     LSAANDINFIVNSAASAGAGTILVTNLPKLSLTPQFRGTAAAPLADFAVTTFNGALLT
                     SVSATAAANPNSNIIVMDLFKIGDVIANNPGQFGVTNVTTACFNGVSLCTDPGYFYFD
                     GVHPTALGHRLIAQLATDYLYYGDLGAQSTVMGETAFRHREDSLDEGSAALSGREEWH
                     EGSSLLVSGSYDRTTSDARGVVGEARSDGYGVRAALENGWNNTWKVGLAGSYRNADVD
                     GARFTTSVDTLAFDVYGGWRSGGAFVNVSAGAAHDEFDIDRLTSLAPIVHTGSTEGVS
                     RG"
BASE COUNT          340 a          628 c          583 g          310 t
ORIGIN      
        1 acccgttcga gagcccgctg atgggactga agaacgtcat cctgaccccg cacatcggcg
       61 gttcgacgga agaggcgcag gaagccatcg ccgagttcgc ggccgagcgg ctgctgggct
      121 atctgaaccg gggcgacacg acgttcagcg tcaacctgcc gaacgtgcag ctgtccgagg
      181 tctcaggcgg acaccgcatc ctgcacatcc acaggaacca gccaggcgtc atggccgagc
      241 tcaaccgcga gctggcgtca gccgggttga acatcctcgg ccagcacctg aagaccgacg
      301 agcgcaccgg ttacgtcatc accgacgtcg atcgcgacta cgatcctgag gcgctgaaga
      361 ccctcaaagg tgttgccgga acgctgcggt tcagactgct tcactaaatc gtcgcgaagc
      421 ttccctgacg gcatcttgag ttcgcagcaa acgagcgcac agtgtccttc aacgaccgcg
      481 tactgacggt cgagggagga acatatgtca ggatttcatc gcggtacggc tttggccgtg
      541 ctcgcagccg ccgcgactct ggccgcagcg tcgggcgcaa gcgcacagac ctacaaccgg
      601 ctggtcgtct tcggcgacag cctgagcgac aacggcaacc tgtatgccgc gaccggaggc
      661 ctgcagccaa cgtcgccacc gtatttccag ggacgcttct cgagcggtcc gaccttcgtc
      721 gagcttctgg ggttcaccct gggccgttcg gtgaccgggg ctccggtcac gggcagcatc
      781 gactatgcat tcggtgggtc gcgcaccgat agtcaggctt tcccgcccgg catgcggctg
      841 cagctccagc aatacacggc agccggaggc aagttcggag cctcagacct cgtcagcatc
      901 ctgggcgggg ccaacaacat cttccagggc ctgccggccg ccggtgcgag cgccaatccg
      961 accggttcca tcagtccggt cgccctttcg gcggccaacg acatcaactt catcgtcaac
     1021 agtgcggcca gcgccggagc ggggaccatc ctcgttacca acctgcccaa gctgagcctg
     1081 acgccgcagt tccggggcac agccgcagcc cctctcgcag acttcgccgt gacgaccttc
     1141 aacggcgcac tgctgaccag cgtcagcgcg acggcggcgg ccaacccgaa cagcaacatc
     1201 atcgtcatgg acctgttcaa gatcggcgac gtcatcgcca acaatccggg acagttcggc
     1261 gtcaccaacg tgaccacggc ctgcttcaat ggcgtctcgc tctgtacgga cccaggctat
     1321 ttctacttcg acggggtcca tccgacggcg ctgggtcacc ggctcatcgc ccaactggcc
     1381 accgactacc tctactacgg cgacctcggc gcccagagca cggtgatggg ggagaccgcg
     1441 ttccggcacc gcgaggacag tctggacgag ggttccgccg ccctgtccgg tcgcgaggag
     1501 tggcatgaag gctcctccct cctggtgagc ggctcatatg atcgcaccac cagcgatgcg
     1561 cggggtgtcg tgggtgaagc caggtcggac ggttacgggg ttcgcgccgc attggagaac
     1621 ggctggaaca acacgtggaa ggtcggtctg gcgggcagct atcgcaacgc cgacgtcgac
     1681 ggcgcacgct tcaccaccag cgtcgatacg ctggcgttcg acgtctacgg cggctggcgt
     1741 tcgggcggcg cgttcgtcaa cgtctctgcc ggcgcggcgc atgacgagtt cgacatcgat
     1801 cgcctgacct cgctcgcgcc gatcgtgcat acgggctcga ccgaaggtgt ttcgcggggc
     1861 g
//
LOCUS       DASVTM010000490         3723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_577840,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000490 DASVTM010000000
VERSION     DASVTM010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577840"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF633_13555"
     CDS             complement(<1..530)
                     /locus_tag="VF633_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX8472131.1"
                     /translation="MKIMLVAAEASGDALGAGLAKALRTRLPDAAFVGVGGPKMAVEG
                     VVSPFDIAELSILGWIEGLRAYDRVKARVADTVALAIRERPDAVVLIDSWGFTIRVAK
                     ALREALPVVPLIKYVGPQVWASRPGRAKTLAGAVDHLLALYTFDAPWFEAEGLATTVV
                     GSSALHVDMDGADGAQ"
     gene            complement(527..1363)
                     /gene="lpxI"
                     /locus_tag="VF633_13560"
     CDS             complement(527..1363)
                     /gene="lpxI"
                     /locus_tag="VF633_13560"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166915.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX8472132.1"
                     /translation="MTQSRLGLIAGGGALPLAIAARCEAEGRPLFVIRLTSFADPHLA
                     RYPGVDLGMAELGKALAALKASGCKAVCFAGTVSRPDFKALKPDLKGAILLPGIIAAA
                     TKGDDALLRKILSVFENEGYAVEGADDILGGETLGAGALGSVSPTVEQLADLKKALHV
                     AEKAGELDIGQGAVVCDGLVLAVEAQEGTDAMLMRVAGLPADLHGSPTSPKGALGKAP
                     KPIQDLRVDMPVIGTRTVELAAAAGLAGVGGVAGQLILIDRQALIETANRLGLFVWGQ
                     TR"
     gene            complement(1360..2148)
                     /gene="lpxA"
                     /locus_tag="VF633_13565"
     CDS             complement(1360..2148)
                     /gene="lpxA"
                     /locus_tag="VF633_13565"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX8472133.1"
                     /translation="MSTVHPTALVHPSAELADGVQIGPWCTVGPGVSLAEGVTLVSHV
                     VVQQDTSIGARTIVHPFAVIGGDPQHNGYKGEPVRLEIGTDNSIREHCTFNRGTPQGS
                     GVTRVGSNGLFMTGAHVGHDAVVGDNVVMANNATLGGHAQIGDKVFLGGLCAVHQNGR
                     VGQGAIVGGLAAVTRDVIPYGSAWGNHAELHGLNLIGLKRKGYGKDAVRRLLAAFRDL
                     FQGEGVFADRLDGVEQTYADLPEIMEIVAFIRDGGKRPLCLPGE"
     gene            complement(2145..2633)
                     /gene="fabZ"
                     /locus_tag="VF633_13570"
     CDS             complement(2145..2633)
                     /gene="fabZ"
                     /locus_tag="VF633_13570"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX8472134.1"
                     /translation="MSDATVNDRIDYAEVMRRLPHRYPFLLVDKAEDFVAGTSITGIK
                     NVTHNEPFFQGHFPIDPVMPGVLIVEAMAQTGALLMSRTLDVAVADKVIMFMSIDGVR
                     FRKPVRPGDQLRMKVIVTRARGDAYKFRGETFVDDKLAAEAEFMAMVVKVDKPASAVT
                     AA"
     gene            complement(2630..3649)
                     /gene="lpxD"
                     /locus_tag="VF633_13575"
     CDS             complement(2630..3649)
                     /gene="lpxD"
                     /locus_tag="VF633_13575"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX8472135.1"
                     /translation="MPDARFFETLPSLTVTTLAEQIGGVVVRGGETDIVSVAPLSSAD
                     GGAVSFLADRKFTAALAETRAGCVIVPAWAVEAVPQGAAIITSGEAQAAWARASLLLH
                     RVRTLDEVAAREQACEDDSVVLEAGVVLGQGARIGLGTRVGANTVIGPGVQIGRYGVI
                     GSNVTLGFTLIGDRVKIYAGARIGEAGFGAAGSKAGAVDIPQLGRVIVQDGVTIGANT
                     CIDRGAYEDTVIGENSKIDNLCMIGHNSVLGRNVLMAAHSGLSGSVTVGDNVMFGGQA
                     GVGDHITIGDGAAVAAGGGVLTNVPAGQVWSGYPAKPLRQFLRETIWVSKQVAHKKGA
                     KDSAG"
BASE COUNT          658 a         1271 c         1173 g          621 t
ORIGIN      
        1 aactgcgcgc cgtccgcccc gtccatatcg acgtgcaggg ccgacgagcc gaccacggtc
       61 gtcgccaggc cctcggcctc gaaccatgga gcatcgaagg tgtagagcgc cagaagatga
      121 tcgaccgctc cggccagcgt cttcgcccgc cccggtcgcg aggcccacac ctgtggaccg
      181 acatatttga tcagcggcac gaccggaagc gcctcgcgca gggccttggc cacgcggatg
      241 gtgaagcccc agctatcgat cagcaccacg gcgtcaggcc gctcgcgaat cgccagcgcg
      301 acagtatcgg cgacccgggc cttaacccgg tcataggcgc gcaagccctc gatccagccg
      361 aggatggaca gttctgcgat gtcgaaaggg ctgaccacgc cctcgaccgc catcttcgga
      421 ccgccgacgc cgacgaatgc ggcgtccggc aatcgggtcc gcaacgcctt ggccagacct
      481 gcgcccagcg cgtcgccaga ggcctcggct gcgaccagca tgatcttcat cttgtctggc
      541 cccagacgaa caggccgagc cggtttgcag tctcgatcag ggcctggcga tcgatcagga
      601 tcaactggcc cgcaacgccg ccgacccccg ccagccctgc tgcggccgcg agttcgaccg
      661 ttcgggtccc gatcaccggc atatccacac gaaggtcctg tattggcttc ggcgccttgc
      721 cgagcgcgcc cttgggagac gtcggcgagc cgtgcaggtc ggcaggcagg ccggcgacac
      781 gcatcagcat ggcgtccgtg ccttcctgcg cctcgaccgc cagcaccagc ccgtcacaga
      841 caactgcgcc ctgaccaatg tcgagttctc ccgctttttc agcgacatgc agggcctttt
      901 tcaggtccgc caactgctcg acagtggggc tcaccgagcc caaagcgccc gccccgagcg
      961 tttcgccacc gaggatgtcg tctgcgccct caaccgcata gccttcgttc tcgaacaccg
     1021 agagtatctt cctcagcaac gcgtcgtcgc ccttggtcgc cgcggcgatg atgccgggca
     1081 gcaggatcgc gcctttcaaa tcgggcttca gcgccttgaa gtcggggcgg cttacggtgc
     1141 cggcgaaaca gaccgccttg cagcccgagg ccttcaacgc cgccagcgcc ttgccgagtt
     1201 cggccatccc cagatccacg cccggatagc gagccagatg gggatcggca aagctggtca
     1261 accggatcac gaacagcgga cgcccttcgg cttcacaccg agcggcaatg gccagaggca
     1321 gggcgccgcc cccggcgatc aggcccagcc gggactgtgt cattcgccag gcagacacag
     1381 cggtcgtttg ccgccgtcgc ggatgaaggc gacgatttcc atgatctccg gcaggtcggc
     1441 gtaggtttgc tcgaccccgt ccagacgatc ggcgaatacg ccctcccctt gaaacagatc
     1501 gcggaaggcg gccagcagac gtcgcaccgc gtccttgccg tagcccttgc gcttgagacc
     1561 gatcaagttg agcccgtgca gttcagcatg attgccccag gccgagccgt aggggatgac
     1621 gtcccgcgta actgcggcca gaccgccgac gatggcgccc tgccccaccc ggccattctg
     1681 gtgcacggcg caaagtccgc cgaggaagac cttgtcgccg atctgcgcat ggccgccgag
     1741 ggtggcgttg ttggccatga cgacattgtc gccgacaacc gcatcgtggc cgacgtgggc
     1801 gccggtcatg aacaggccat tggagccgac gcgggtcacg ccagagcctt ggggcgtgcc
     1861 ccgattgaag gtgcagtgct cgcggatgga gttatcggtg ccgatctcca gtcgcacggg
     1921 ctcaccctta tagccgttgt gctgcgggtc gccgccgatg acggcgaagg gatggacgat
     1981 ggtgcgggcg ccgatgctgg tgtcctgctg gaccacgacg tggctgacga gggtcacgcc
     2041 ttcggcaagc gacacgccgg ggccgactgt acaccacggt ccgatctgca ccccgtcggc
     2101 gagctcggcc gaaggatgaa caagcgcagt cggatgaacc gtgctcacgc tgcggtgacc
     2161 gcggaggcgg gcttgtcgac cttgaccacc atagccatga actcggcctc ggcggcgagc
     2221 ttgtcgtcca cgaaggtctc gcctctgaac ttgtaggcgt cgccccgggc acgggtgacg
     2281 atgaccttca tgcgcagttg gtcgcccggt cgcacaggct tgcggaagcg cacgccgtcg
     2341 atcgacatga acatgatgac cttgtcagcc accgcgacgt ccagtgttct ggacatcaga
     2401 agagcacccg tctgggccat ggcctcgacg atcaggacgc ccggcatgac cggatcgatg
     2461 gggaaatgcc cctgaaagaa gggctcgttg tgggtgacgt tcttgatgcc ggtgatcgag
     2521 gttccggcga cgaaatcctc ggccttgtcg acgagcagga aggggtatcg gtgcggcagg
     2581 cgtcgcatga cctcggcata gtcgatccgg tcattcacgg ttgcgtcgct catccggccg
     2641 aatccttcgc gcctttctta tgggcgacct gtttggacac ccagatcgtc tcgcgcaaga
     2701 attgtctaag cggtttcgca ggataaccgg accagacctg gcccgcaggt acgttcgtca
     2761 gcaccccgcc accggccgcg accgctgcgc cgtcaccgat ggtgatgtgg tcacccacgc
     2821 cggcctgacc gccgaacatg acgttgtcgc ctacagtcac cgatccggag agtccgctgt
     2881 gggcagccat cagcacgttg cggcccagaa cgctgttgtg cccgatcatg cacaggttat
     2941 cgatcttgga gttctcgccg atgactgtgt cttcataggc cccacggtcg atgcaggtgt
     3001 tggctccgat ggtgacgccg tcctgcacaa tgacgcgacc gagctgcggg atgtcgacag
     3061 ctccggcttt cgacccggcg gcgccgaagc ccgcctcgcc gatgcgggcc ccggcataga
     3121 tcttcacccg gtcgccgatc agggtgaaac cgagggtgac gttggagcca attaccccgt
     3181 agcggccgat ctggacgccc ggaccgatga ctgtattcgc gccgacacgc gtccctagac
     3241 cgatgcgcgc gccctgcccc agcacgacgc cggcctcaag aacaacgctg tcgtcctcgc
     3301 aggcctgttc tcgagcggcg acctcatcca gcgtgcgcac gcgatgaagc agtagtgacg
     3361 cgcgagccca agctgcctgg gcctcgcccg aggtgatgat agcggcgcct tgcggaactg
     3421 cctcgaccgc ccacgccggg acgatcacgc atccggcccg cgtctcagcc aaggccgccg
     3481 tgaacttgcg gtcggccaaa aacgacaccg cgcccccgtc cgcagaggac aggggcgcga
     3541 cggagactat atcggtctcg ccacctcgaa cgaccacgcc gccgatctgt tcggccagcg
     3601 tggtcaccgt cagggacggc agggtctcga agaaccttgc atcgggcatg atgaaacgcc
     3661 cgctttggtt cttactggcc ttgtggctga accggaaccg tcatgcggtt gaaggtcagc
     3721 gac
//
LOCUS       DASVTM010000491         5498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_151787,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000491 DASVTM010000000
VERSION     DASVTM010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151787"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VF633_13580"
     CDS             complement(<1..901)
                     /locus_tag="VF633_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX8472136.1"
                     /translation="MTSSRLRTLLSSLSLWVVLALVLGLAVGAIAQGTGIERIVTTIE
                     PLGGVWLNALRITVIPLVFAMLVTGIVSIADAASTGRLAARALLVFAIMLVAATTYAI
                     LAGLGLLNLWPIDPEAGRALLAGISADTTVTVGEAAKTDGLQAFLSSLAPSNLVKAAA
                     EDGVLGVVVFAIAFGFAATRLEARLRQPLAVFFEATAEAMVVIVHWVLLAAPLGVFAL
                     ALGVGLRAGVGAAGVLGHYIAIVCLSQIGVIILVYLGVLVFGRIGLRRFVIAAAPAQV
                     VAFSTQSSLASLPVMVERARDALG"
     gene            993..3155
                     /locus_tag="VF633_13585"
     CDS             993..3155
                     /locus_tag="VF633_13585"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX8472137.1"
                     /translation="MRSLPAIILAAIVTSGTALAVLASGAPAAAQTRPLTVAASGRTW
                     ANPVDIDYRFSADHAQPGISYRTGGDPTIVLHRDRYYLFQTSGEGYWTSRNLVDWTYI
                     TPDLRLFGTSIAPSVASDDEALFLMQSSLEPRPLLSSVNPVEGAWNVHTPLMPSIPGA
                     DAKGADVVLAQDHITAGPWSPDLFIDDDGRWYVYWGSSDTFPLYGSAIDPNSPMKWVG
                     APRPFFALDPEQHGWERFGQDHSGGLPDGTAVRPFLEGAWMTRRSGRYYLQYGAPGAE
                     YNAGANGVYVGSTPLGPFEYAPYSPVSYKPGGFVTGAGNGSTFQDRYGNWWNTGGAWV
                     GVNWSLERRIAMFPGSFHDDGQMWFSSRFGDFPQRMPDGPVTDPESLFTGWMPLSYRA
                     KSTASSTLDDFVADRATDEDPRTFWVAADNVPGQTLTLDLGALKTVEAVQINYADYQA
                     GVFTEEPGLRTRFNILLSVDGETWAPFANRMTSDRDSPNAYIQGDRPVLARYVRYEHG
                     ETPGARLAVADFRVFGTAAGDLPAAPANVIVKRGDDQRNASVTFDTVPNPAGGRILGY
                     NVRWGVKSDRLHLTYQVWADDLAARGGSVDIRALNTGVDYWFAVEAFGDTGVSPLSVV
                     LEVPGGAPVTVREPEPPFTGLPQPADPGKPPLTGTVTTPIPPVYISGAPPVIGSALPP
                     AGTEATAPAMTPDTTQPSSPVPEQGDAVDEDATSALSH"
     gene            3202..3420
                     /locus_tag="VF633_13590"
     CDS             3202..3420
                     /locus_tag="VF633_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease"
                     /protein_id="HEX8472138.1"
                     /translation="MALSRIATAVGGLLLSKGGRRFAGRHAGKLAIATLAFELWQQHR
                     NKTVTPDAAAAQPGARPQSRTRWSGRRT"
     gene            3503..4204
                     /locus_tag="VF633_13595"
     CDS             3503..4204
                     /locus_tag="VF633_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8472139.1"
                     /translation="MLTVHHLNNSRSQRVLWLLEELGVPYEVKRYERNPATMLAPPEL
                     RAIHPLGKSPVIEENGVVTAETGAIIEQILSEIPGNALKPAPGTEGARRLTYWLHYAE
                     GSAMTPLLLKLVFGALPNRSPGLMKPLVNAISKKAQSGFIDPQIAAHTAYWEGELSKT
                     AWFAGDQFTAADIMMSFPLEACSSRAPFGLDRPRCSAFLKAIHARPAYQRALERGGPY
                     AYARGGQSVLPATAG"
     gene            4286..4486
                     /locus_tag="VF633_13600"
     CDS             4286..4486
                     /locus_tag="VF633_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472140.1"
                     /translation="MRIQTVQIVAALIAAIAFVIAFISAGVAVISGMFALVGAASLAW
                     LVYTIAKRMLLSRSERNATPTA"
     gene            4651..5124
                     /gene="greA"
                     /locus_tag="VF633_13605"
     CDS             4651..5124
                     /gene="greA"
                     /locus_tag="VF633_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8472141.1"
                     /translation="MEKVPMTGEGYRSLDDQLKQLKSVERPSVIAAISEAREHGDLSE
                     NAEYHAAKERQGWIEGQIAEIEDKISRAQVIDVSKLSGSVVKFGATVTVVDEDTEEEG
                     RYQIVGEHEADVKKGKISISSPIARAMISKDVGDVVEVNTPGGVKAYEILKVEWK"
     gene            5151..>5498
                     /locus_tag="VF633_13610"
     CDS             5151..>5498
                     /locus_tag="VF633_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HEX8472142.1"
                     /translation="MRIWVVSDGRTGMENQALGLAEAIQRLTPADITVKRICWRPLFD
                     RLPSALKTPAMLDPSSDAPFPAAAEPWPDLWIATGRATLPLSLAVRRRSGGKTFVVQT
                     QNPRGSLSQFDMVV"
BASE COUNT         1033 a         1857 c         1663 g          945 t
ORIGIN      
        1 cgccgagtgc atcgcgtgcc cgctcgacca tcaccggcag gctggcgagc gaagactggg
       61 ttgagaaggc cacgacctga gccggggcgg cggcgatcac gaaccgcctc aagccgatcc
      121 gtccaaacac cagcacgccc agatagacga ggatgatcac cccgatctgg cttaggcaga
      181 cgatggcgat gtagtggccc agcaccccgg cggccccgac cccggcgcgc agccccacgc
      241 ccagggccaa agcgaacacg cccagcggcg cggccagaag cacccagtga acgatgacca
      301 ccatggcctc tgccgtcgcc tcgaagaaga cggcaagcgg ttgacgtagg cgggcctcca
      361 gccgggtcgc ggcgaaacca aacgcgatcg cgaagaccac gacgccaagc acgccgtcct
      421 cggcggccgc cttgaccaga ttcgatggcg cgagactgct gaggaacgcc tgcagcccgt
      481 ccgtcttcgc cgcttctccg accgtgaccg tcgtatcggc cgatattccc gccagaagcg
      541 cacgtccggc ctctgggtcg atgggccaca agttcagcag gcctaatccc gcgagaattg
      601 cgtaggtcgt cgccgcgacc agcatgatcg cgaacaccag caacgccctg gccgccagcc
      661 tgccggtcga ggccgcatcg gcgatcgaaa cgatcccggt caccagcatg gcgaacacca
      721 gagggatgac cgtgatcctc agcgcgttca accagacccc gcccagcggc tcgatcgtgg
      781 tgacgatgcg ttcgatgccg gttccttggg cgatagctcc gacagccaac ccgaggacaa
      841 gagcgagaac cacccagaga ctgagcgaag acaacagggt acgaaggcgc gaagaggtca
      901 tttgcgacac ctagcactct cgtcgggcga tgccacctgc cgacggttgt tcgaagacta
      961 tcgctccatg acgctcatgc tagggtccag gtatgcgttc gttgcccgcc attattttag
     1021 ctgccatcgt gaccagcggg accgccctgg ccgtcctcgc cagcggcgcc cctgccgccg
     1081 cgcagacacg cccgttgact gtggcggcct ccggccgaac ctgggccaat ccagttgata
     1141 tcgattatag attcagcgct gaccatgcgc agcccggcat cagctatcgc acgggcggcg
     1201 acccgacaat cgtccttcac cgcgaccgtt actacctgtt ccagacctcc ggtgagggct
     1261 actggacatc gcgcaatctg gtcgactgga cctatatcac tcccgatctc cgactgttcg
     1321 gaacgagcat cgcgccgagt gtcgcttccg acgatgaggc cctgttcctg atgcagtcgt
     1381 cgctcgaacc acgtcctctg ttgtccagcg tcaatccggt cgagggagcc tggaatgtgc
     1441 acacgccgct gatgccgtcg atccccggcg cggatgcaaa aggcgcggac gtcgtgctgg
     1501 cccaggacca tatcaccgcc ggtccgtggt cgccggacct gttcatcgac gacgacggca
     1561 gatggtacgt ctactggggg tcgtccgaca ctttcccgct gtatggctcg gcgatcgatc
     1621 cgaactctcc gatgaagtgg gtgggagccc cccgcccctt cttcgcgctt gatcccgagc
     1681 agcacggctg ggagcggttt ggtcaggacc actccggcgg cctgccggac ggcaccgcgg
     1741 tccgcccgtt cctcgaaggg gcatggatga cccgcagaag cgggcggtac tacctgcaat
     1801 acggcgcacc cggcgcggaa tacaacgccg gcgccaacgg ggtgtatgtc ggctcgaccc
     1861 cgcttggccc gttcgaatat gcgccctaca gcccggtcag ctacaagccc ggcggtttcg
     1921 ttaccggggc tggcaacggc tcgaccttcc aggaccgtta cggcaactgg tggaacacgg
     1981 gaggcgcctg ggtcggcgtc aactggagcc tcgagcggcg cattgcgatg ttcccgggca
     2041 gttttcacga cgacggacag atgtggttct cgagccggtt cggcgacttt ccgcaacgga
     2101 tgcccgatgg ccccgtcacg gaccccgaga gcctgttcac cggctggatg cccctgtcct
     2161 accgtgcaaa atcgacggcc tcttcgacgc ttgacgactt cgtcgccgac cgcgccacgg
     2221 acgaagaccc gcgcaccttc tgggtcgccg ccgacaacgt tccgggacag acgctgaccc
     2281 tcgatctcgg cgcgctgaag acggtcgagg cggttcagat caactatgcc gactatcagg
     2341 ccggcgtctt cacggaagaa ccgggcctgc ggacccgctt caacatcctg ttgtccgtcg
     2401 acggagagac atgggcgcca ttcgccaacc gcatgacttc cgaccgcgac agccccaatg
     2461 cctatatcca gggcgaccga ccggtgctgg cccgttatgt ccgttatgag catggcgaga
     2521 cgccgggcgc ccgtctggcc gtcgccgatt tccgcgtgtt cgggaccgca gcgggcgatc
     2581 ttcctgcggc tcccgccaat gtcatcgtca aacgcggtga cgaccagcgg aacgcgagcg
     2641 tcaccttcga cactgtaccg aaccccgccg gcggccgcat cctcggctac aacgtccgtt
     2701 ggggtgtaaa gtccgaccgg ctgcacctga cctaccaggt ctgggccgac gatctggctg
     2761 cgcgtggcgg atcggtggat atccgcgcgt tgaatacggg tgtcgactac tggttcgcgg
     2821 ttgaagcctt cggcgacacc ggcgtttcgc cactcagtgt cgtgctggag gttccgggtg
     2881 gcgcgccagt cactgtccgc gagccagagc cgccctttac cggcctgccg caacctgctg
     2941 accccggcaa gcctccgctg accggcaccg tcacgacgcc catccccccg gtgtacatca
     3001 gcggcgctcc acccgtgatc ggttcggcgc ttccgccagc cggaaccgag gcgacagcac
     3061 cagcgatgac gccggacaca acacaaccct cctctccggt tccagaacaa ggggacgctg
     3121 ttgatgaaga cgcgacgtct gctctcagtc actgaaccct tgtccgttgt ggccgttgat
     3181 gtggccaacc ggagttaatt catggccttg tcccgtatcg ccactgccgt cggcggcttg
     3241 cttctgtcaa agggcggacg tcgttttgcc ggtcgtcatg ccggcaagct ggctatcgcc
     3301 accctggcct tcgagctgtg gcagcagcac aggaacaaaa ccgtcacgcc agacgcggcg
     3361 gcagctcaac ccggcgcccg gccacaaagc cgcacacgtt ggagcggccg gcgcacctga
     3421 gagtatgaac gctgcttgcc gtcccctgcg tcaggcgccc tagagtgctg cagctagcga
     3481 acacttcgca gggaaacgcg ccatgctgac cgtccaccac ctgaacaact cccgctctca
     3541 gcgcgtgctc tggctgctgg aagaactggg cgttccttac gaggtcaaac gctacgagcg
     3601 gaaccccgcg acaatgctcg cgccgcctga actgcgagcc atccacccgc tcggcaaatc
     3661 gcccgtgatc gaggaaaatg gcgtcgtcac tgcggaaacg ggcgcaatca tcgaacaaat
     3721 cctgtcggag atccccggca acgcacttaa gcctgccccc ggcacggaag gggcgcgccg
     3781 cctcacctac tggctgcact atgcggaagg ttcggccatg acgcccctgc tgctcaagct
     3841 ggtcttcgga gccttgccaa accggtctcc cggattgatg aagccgctgg tcaacgccat
     3901 ttcgaagaag gcccagtccg gcttcatcga tcctcagatc gcggcccaca ccgcctactg
     3961 ggaaggcgaa ttgtcaaaga cggcatggtt cgcaggcgac cagtttaccg cagccgacat
     4021 catgatgagt ttcccgctcg aggcctgttc ctcccgcgcg ccgttcggtt tggacaggcc
     4081 acgatgcagc gctttcttga aagccatcca cgcacggccg gcgtatcagc gggcgctgga
     4141 gcgtggcggt ccgtacgcct atgcgagagg gggccaaagc gttctgccag cgaccgccgg
     4201 ctgatgcgcc tttaccatca gcacaacgtc gtgagatgga tcgctgacat agcaaccgcg
     4261 acaggccagg tgcgttcaac gggtcatgcg tatccagacc gttcaaatcg ttgccgccct
     4321 catcgcggcc atcgccttcg tcatcgcgtt catctctgcg ggtgttgcgg tgatctcagg
     4381 catgttcgcg ctggtaggag ccgcctctct cgcctggctg gtatatacga tcgccaagcg
     4441 catgctgctg agcagaagcg agcgcaacgc cacgccgacc gcctgaaacc agccggataa
     4501 cgcgggtcgc tccagcgact tgagtttagt attcaggcgc cctagtctct ttggccggtc
     4561 gcacgcgccc agcgactgca gcctagaaga cgtctttcct cactgtcctc cctccgggcc
     4621 cccgggcgaa acaagaaact tctgatacat atggaaaaag tgccgatgac gggcgagggc
     4681 taccgctccc tcgacgatca actgaagcaa ttgaagtcgg tggaacggcc cagcgttatc
     4741 gcggccatct ccgaagcccg cgaacacggc gacctttccg agaacgccga gtaccacgca
     4801 gccaaggagc gtcagggctg gatcgaaggt cagatcgccg agatcgagga caagatcagc
     4861 cgcgctcagg tcatcgacgt gtccaagctg tccggcagcg tggtcaagtt cggagccacg
     4921 gtcaccgtgg tcgacgagga cactgaggaa gagggtcgct accagatcgt cggtgagcac
     4981 gaagccgacg tgaagaaggg caagatcagc atttcgtcgc cgatcgctcg cgccatgatc
     5041 tccaaggatg tcggcgacgt ggtcgaggtc aacacgcccg gtggcgtgaa ggcctatgag
     5101 atcctcaagg tcgagtggaa gtaagccact ctcctgaagt tgtcgggacc ttgcgtatct
     5161 gggtcgtctc cgacggccgt acaggcatgg aaaatcaggc gctgggcctt gctgaggcga
     5221 tccagcgtct gacccctgcg gacatcaccg taaaacgcat ttgctggcgt ccgctgttcg
     5281 acaggctgcc gtccgccttg aagacgccgg ccatgctgga ccccagctct gacgcgccct
     5341 ttcccgctgc cgcagagcct tggccggatt tgtggatcgc aacgggtcgc gcgacgctcc
     5401 ctctatcact cgccgtccga cgccgatccg gcggcaagac cttcgtggtc caaacccaga
     5461 acccacgtgg gtcgctgtca cagttcgaca tggtggtg
//
LOCUS       DASVTM010000492         6078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_416933,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000492 DASVTM010000000
VERSION     DASVTM010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416933"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VF633_13615"
     CDS             complement(<1..404)
                     /locus_tag="VF633_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472143.1"
                     /translation="MSGPVRVLVVDDSPTMRGLITAALRRDPEIEVVGTAADPLEARA
                     LIKTLNPDVITLDIEMPNMNGLEFLEKIMRLRPMPVVMVSTLTQAGAEITLDALEMGA
                     VDAVGKPGAGVTAMEAFAELTLKVKTAARSQVR"
     gene            complement(401..1288)
                     /locus_tag="VF633_13620"
     CDS             complement(401..1288)
                     /locus_tag="VF633_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HEX8472144.1"
                     /translation="MSTGSSSKSGAGRESLVEGEFVFTAEDFRNIAEILHSYAGIALN
                     EGKAALVYSRLAKRLRTLGLQSFRDYCALVQGADGVDERQTMTAALTTNVTRFYREPH
                     HFDHLRDQVMPDLIERARKGGRVRLWSAACSNGQEPYSMAMTVLSVLPEAADLDVRIL
                     ATDIDPNMVAEGAAGVYSEEALEPAPAMLRNRFFDKVAGAGGQTMLSANSTLRDLVSF
                     RELNLIGQWPMRGKFDAIFCRNVVIYFDDATQERVWKRFTPLMNPGATLYIGHSERVS
                     GPATSQLQTSGLTTYRLGA"
     gene            complement(1285..1743)
                     /locus_tag="VF633_13625"
     CDS             complement(1285..1743)
                     /locus_tag="VF633_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX8472145.1"
                     /translation="MPDVSNAPSRELIAFRIGEQEFCVDIMSVREIRGWTPATPIPRA
                     PGFMKGVINLRGAVLPIIDLGARFGLRTSDPTARHVIMVAHIGGRMVGLLVDAVSDII
                     QLTDEALQPTPDIASEQVKSFVKGLFALDGRLISLIELEQIIPEVEAEAA"
     gene            complement(1746..4001)
                     /locus_tag="VF633_13630"
     CDS             complement(1746..4001)
                     /locus_tag="VF633_13630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX8472146.1"
                     /translation="MSDAVDPFEAIKVVFFQECDELLGDLEAGLLAIESGEADDETVN
                     AVFRAVHSVKGGAGAFGLDALVRFAHVFETLLDEIRSGRKPCDDPTVKTLLRASDVLA
                     DHIAAARGQAAPVDEARSAGLVAELEMLTHGEARTQPSEAPAPTETVVAEEDEFGFRP
                     VAFSFSGVIEDSPVETPASDVWRIVFRPHGRMYVNANETGLLLRELSRLGEMTVSLDA
                     SAVPDLSELVAEEGYLSWTISLATDAGEAAIRDVFDFVESDCDLSISAAPSGIEEPPS
                     VPDDGFDIAALLMRAQADVPAPSEEVIAPPATGPVIPSEPVAQKPSAPKPAVAVPAPP
                     PAAPVAPAAVTIRVDLDRVDRLINVVGELVIQQAMLAQRVIESGLARSSGISIGLEDL
                     ELLTREIQDSVMAIRAQPVKSVFQRMPRLAREVGEMTGKRVRLITAGEDTEVDKTVVE
                     RLAEPITHMLRNAIDHGLESPDDRIAAGKPAEGTVRLAALHRSGRIVIEISDDGRGIN
                     RERVKGIAISRGLIPEDAVLTDEETDNLIFLPGFSTAETITDVSGRGVGMDVVRRSIQ
                     ALGGRISISSIPGKGSTFTLSLPLTLAVLDGMVVTAAEQTLVAPLTAIVESLTPRVQD
                     IHYVGGHDAVIRFRDQFVPLIDVALGMGFRETPIAPETGVAVVIETEAGLRAALLFDA
                     IQGQRQVVIKSLETNYQQVEGVAAATILGDGRVALILDCDAVVASSRKKSVRPELARP
                     DAIRPDLKLAS"
     gene            complement(4004..4366)
                     /locus_tag="VF633_13635"
     CDS             complement(4004..4366)
                     /locus_tag="VF633_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472147.1"
                     /translation="MKTVLTIDDSRTMREMLMMALVEAGYRVIQAVDGIDGLETLSAE
                     GADVIITDINMPRLDGFGVIEGVRADPNHRATPVLVLTTESDVAKKQRARDAGATGWI
                     VKPFDPVKLVDAVRRVAA"
     gene            complement(4363..4644)
                     /locus_tag="VF633_13640"
     CDS             complement(4363..4644)
                     /locus_tag="VF633_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8472148.1"
                     /translation="MTAPAQVTLPPVLDLQHAEPLRAELLNLRGQAVVIDGSAVERLG
                     GLCLQVLISAQKSWAEDGQVMSLDRVSEAFADQWNMFGAVMADEPGEIA"
     gene            complement(4644..>6078)
                     /locus_tag="VF633_13645"
     CDS             complement(4644..>6078)
                     /locus_tag="VF633_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8472149.1"
                     /translation="SAATSAAILSLIIGIVAALLISLVAGVVLSRSIATSILGLISEM
                     KRLVGGDTSIGVPQASRKDEIGQMGVALIAFRDAAIEKNRIEAEALANRSQSEIERAA
                     SEAEKARVAAEDAVAITALGEGLSAIADGDLTHRFTAQVAARSEGLKTNFNTAVVQLQ
                     DTMSVVVTNVSAIRSGSGEISQAADDLSRRTEQQAASLEETAAALDEITATVNRTASG
                     ARQASDTVQAAKGDAETSGNVVRDAVTAMGAIEKSSQEISQIIGVIDEIAFQTNLLAL
                     NAGVEAARAGDAGRGFAVVASEVRALAQRSAEAAKEIKVLISASSTQVKTGVSLVGQT
                     GEALQRIVSRVAEIDGLVSEIAASAQEQATGLQQVNTAVNQMDQVTQQNAAMVEESTA
                     ASHSLSQEAESLASSVARFNIGAQNGAQGGVRAAQPARPAPRPVHRPAQPAPSAHRPV
                     AAMRTLGHGGAARRPEPAAADEGWEEF"
BASE COUNT         1034 a         1926 c         2045 g         1073 t
ORIGIN      
        1 cggacctggg accgggctgc ggttttcacc ttcagcgtca gttccgcaaa ggcctccatc
       61 gcagtgacgc cggcgccggg cttaccgacc gcatcgaccg cgcccatttc gagggcatcc
      121 agcgtgatct cggcgccggc ctgggtcagg gtcgaaacca tgaccacggg catgggccga
      181 agccgcatga tcttctcgag gaactccagc ccgttcatat tgggcatctc gatgtcgaga
      241 gtgatgacgt cggggttcaa cgtcttgatc agcgcgcgcg cctccagcgg atcggcggcg
      301 gtgcctacga cctcgatctc cggatcgcga cggagcgcgg ccgtgatcag gccccgcatg
      361 gtcggcgagt cgtcgacgac cagaactcga accggcccac tcatgcgccg agcctgtagg
      421 tcgtgaggcc gctggtctgc agttgcgatg tcgcgggtcc gctgacacgc tcggaatggc
      481 cgatatagag tgtcgctcct gggttcatca gcggcgtgaa ccgcttccag acccgctcct
      541 gggttgcgtc gtcgaagtag atcacgacgt tgcggcagaa gatcgcgtcg aacttcccgc
      601 gcatcggcca ttggccgatc aggttcagtt cccggaacga gaccaggtcg cgcagcgtcg
      661 agttcgccga cagcatcgtc tggccgccag ccccggctac cttgtcgaaa aaccgattgc
      721 gcagcatcgc cggcgccggc tccagcgcct cttccgaata tacgcccgca gcgccctcgg
      781 cgaccatgtt gggatcgatg tcggtggcca ggatccgcac atccagatcg gcggcttcag
      841 gcaggaccga caggaccgtc atagccatgg aatagggctc ctgaccgttt gagcaggctg
      901 cggaccaaag acgtacgcgt ccgcccttgc gcgcccgttc gatgagatcg ggcatcacct
      961 ggtcacgcag atgatcgaaa tggtggggct cgcggtagaa gcgcgtgacg ttggtcgtca
     1021 gggcggcggt catcgtttgc cgttcgtcta cgccgtccgc gccctgtacg agggcgcagt
     1081 agtcgcgaaa gctctgcaga ccgagggtgc gcaaccgttt ggccagccgc gaatagacca
     1141 gcgcagcctt gccctcgttc agggcgatgc ccgcgtagct gtgcaggatc tctgcgatgt
     1201 tgcgaaagtc ttcggcggtg aagacgaatt cgccttcgac gagggattcg cgccctgcgc
     1261 cggactttga gcttgaaccc gtactcatgc cgcttcggct tccacttcgg ggatgatctg
     1321 ctccagttcg atcaggctga tcaaacggcc gtcgagggcg aacaggcctt tgacgaagga
     1381 tttcacctgt tcgctggcga tgtcgggcgt cggctgcagc gcttcgtcgg tcaactggat
     1441 gatgtccgac acggcgtcga ccaacaggcc gaccatccgc ccgccgatgt gagcgaccat
     1501 gatcacatga cgcgccgtcg gatccgacgt gcgcagacca aaacgtgcgc cgaggtcgat
     1561 gatgggaagc acggcgccgc gcagattgat gacgcccttc atgaagccgg gcgcgcgagg
     1621 aatgggcgtg gcgggggtcc agccgcggat ttcacgcacg gacatgatgt ccacgcaaaa
     1681 ctcctgctcg ccgatacgga aggcgatgag ttcgcgcgag ggggcgttgg aaacgtcggg
     1741 catgatcagc tcgcgagttt gagatcaggg cggatggcgt cggggcgcgc caactcgggg
     1801 cggaccgatt tcttccgtga ggaggcgacg acggcgtcgc agtcgaggat cagggcaacc
     1861 cgcccgtcac ccagaatggt ggcggcggcg acgccctcga cctgctggta gttggtctcg
     1921 aggctcttga tcacgacctg acgctggccc tgaatggcgt cgaacagaag ggcggcgcgc
     1981 aggccagctt cggtttcgat gacgaccgct acgcccgtct ccggcgcgat cggcgtctcg
     2041 cggaaaccca tccccagtgc gacgtcgatc agggggacga actggtcgcg gaagcggatc
     2101 accgcgtcgt gcccgccgac atagtgtata tcctgcacgc gcggcgtcag gctctcaacg
     2161 atggctgtca gcggcgcgac gagggtctgt tcggctgcgg tgacgaccat cccgtcgagg
     2221 acggcgagcg tcagcggcag gctgagcgtg aacgtggagc ccttgcccgg gatcgagctg
     2281 atcgagatgc gcccgcccag agcctggatc gagcggcgga ccacatccat gcctacgccc
     2341 ctgcccgaaa catcggtgat ggtctcggcg gtcgagaagc cgggcaggaa gatcaggttg
     2401 tcggtctctt cgtcggtcag gactgcgtct tcgggaatca gcccccgaga gatggcgatg
     2461 cccttcacgc gttcgcggtt gatgccgcgg ccgtcatccg agatttcgat gacgatgcga
     2521 cccgagcggt gcagggcggc cagacgcacg gtgccttcgg caggcttgcc ggcggcgatg
     2581 cggtcgtcag gactctcaag cccgtggtcg atcgcattcc tcaacatgtg agtgatcggc
     2641 tcagccagac gttcgaccac ggtcttgtcg acctcggtgt cctctccggc ggtgatcagg
     2701 cggacccttt tgccggtcat ctcgccgact tcgcgggcga gccgtggcat gcgctggaac
     2761 acggacttca ccggctgggc gcggatcgcc atgacgctgt cctggatttc ccgggtcagc
     2821 agttcgagat cctcaagccc gatcgagatg ccggaggaac gtgccagtcc gctctcgatc
     2881 acccgctgcg cgagcatcgc ctgctggatg acgagttcgc cgacgacgtt gatcaggcga
     2941 tcgacacggt ccagatcgac gcgaatggtc accgcggcgg gtgcgaccgg tgcggccggt
     3001 gggggagcag ggacagcaac ggccggcttc ggcgccgaag gcttttgggc caccggttcc
     3061 gacgggatca ccggcccggt agcggggggt gcaatgactt cttcagacgg agcggggaca
     3121 tccgcctggg ctcgcatcag caaggcggcg atgtcgaaac cgtcatccgg aacggacggc
     3181 ggctcttcga tcccggaagg ggccgcgctg atggacaggt cacagtcgct ttcgacgaaa
     3241 tcgaagacgt cacggatcgc ggcttcgccg gcgtcagtcg ccagcgaaat cgtccaggag
     3301 agatagcctt cctcggcgac cagttcagac aggtcgggca ctgcagaggc atcaagcgac
     3361 accgtcatct cgcccagccg cgaaagctcg cgcaacagca gacccgtttc gttggcgttg
     3421 acgtacattc ggccgtgggg ccggaaaaca atccgccaga cgtcgcttgc gggcgtctcg
     3481 acgggcgaat cctcgatcac ccccgagaag ctgaacgcca ccggccggaa accgaactcg
     3541 tcttcctctg cgacgaccgt ctccgtcggc gcaggggcct cgctgggctg ggtgcgggcc
     3601 tcgccgtggg tcagcatttc cagttcggcg accagtccgg cggagcgggc ctcgtcgacc
     3661 ggcgctgcct gtccgcgcgc cgcagcgatg tgatccgcca gaacgtcgga agctcggagc
     3721 agggtcttta ccgtaggatc gtcgcagggt ttgcggcccg agcggatctc gtccagcaag
     3781 gtttcaaaga cgtgggcgaa ccgcaccagg gcgtcgagac caaaggctcc tgcgccaccc
     3841 ttgaccgaat gcaccgcgcg gaacacggcg ttcaccgtct cgtcgtcggc ctcgccggac
     3901 tcgatcgcca gcaggccggc ctcgaggtca ccgagcagtt cgtcgcactc ctggaagaag
     3961 acgaccttga tggcttcaaa agggtccacg gcgtcgctca tgctcaggct gccacgcgcc
     4021 ggacagcgtc aaccagcttg accgggtcga aaggtttgac gatccagccg gtagcgccgg
     4081 cgtcacgtgc gcgctgcttc ttggcgacat cgctttcggt ggtcagcacc agcaccggag
     4141 tcgcgcgatg attgggatcg gcgcggacac cctcgatcac gccgaagcca tccagtcggg
     4201 gcatgttgat gtcggtgatg atgacatcgg cgccctctgc gctgagtgtc tcaagtccgt
     4261 cgatgccatc gacggcctgg atgacgcgat atccggcctc gaccagggcc atcatgagca
     4321 tttcgcgcat ggtgcgggaa tcgtcgatgg taagaacggt cttcacgcga tttctccggg
     4381 ctcgtcggcc atgacggccc cgaacatgtt ccattggtcc gcaaaggctt cggagacacg
     4441 gtcgaggctc atcacctgac catcctcagc ccagctcttt tgcgccgaaa tcagcacctg
     4501 caggcaaagc ccgcccagcc gttcgaccgc cgatccgtca atcacgaccg cctgtcctcg
     4561 aaggttcagc aactcggccc gcaagggctc cgcgtgctgg agatcgagga ccgggggcaa
     4621 agtcacctgt gctggcgcgg tcatcagaac tcttcccaac cttcgtcagc cgcagccggt
     4681 tcgggccggc gagctgcgcc gccatggcca agggttcgca tagcggcgac cgggcggtgg
     4741 gcggatggag caggttgggc aggacggtga accgggcgcg gcgcaggccg ggcgggttgt
     4801 gccgctcgca cgccgccctg ggcgccattc tgggcgccga tattgaagcg ggcgacagag
     4861 ctggcgaggg attcggcctc ttgcgacagc gagtggctgg cggcggtgga ttcctcgacc
     4921 atggcggcgt tctgctgggt cacctgatcc atctggttga cggcggtgtt gacctgctga
     4981 aggccggtcg cctgttcctg ggcggaggcg gcgatttcgg agaccagacc gtcgatctcg
     5041 gcgacgcggc tgacgatgcg ttgcagcgcc tcaccggtct gacccaccag gctgacgccg
     5101 gtcttgacct gggtcgagga tgccgaaatc agcaccttga tctccttggc cgcttcggcg
     5161 gagcgctggg ccagggcccg cacttcggag gcgacgacag cgaaaccgcg gccagcatca
     5221 cccgcacggg cggcctcgac gccggcgttg agggccagca ggttggtctg gaaggcgatt
     5281 tcgtcgatca cgccgatgat ctggctgatt tcctgcgacg atttctcgat cgcgcccata
     5341 gccgtgactg cgtcgcgaac cacgttgccg ctggtctctg cgtcgccctt ggcggcctgg
     5401 acggtatcgg aagcctgacg ggcgccagag gcggtgcggt tgaccgtggc ggtgatttcg
     5461 tcgagggcgg cggcggtctc ctccagcgaa gcggcctgct gttcggtacg acgcgacagg
     5521 tcgtcggcgg cctggctgat ctcgcccgat cccgaccgga tggccgagac gttggtgacg
     5581 acgaccgaca tcgtgtcctg aagctgaacc accgcagtgt tgaagttggt cttcagtcct
     5641 tccgaccggg ccgcgacctg ggcggtgaag cggtgtgtaa ggtctccgtc ggcgatcgcc
     5701 gacaggcctt cgcccaaggc cgtgatggcc acggcgtcct ccgcggcgac gcgtgccttc
     5761 tcggcctcgc tggcagcacg ttcgatttcg gactgcgatc ggttcgccag cgcctcggcc
     5821 tcgatacggt tcttctcgat tgcggcgtca cggaacgcga tcagagccac gcccatttgg
     5881 ccaatctcat ccttgcggga cgcctggggc acgccgatgc tggtgtcgcc accgacaagt
     5941 cgcttcatct cgctgatcag gccaaggatc gaggtggcga tcgaacggga gaggacaacc
     6001 cctgcgacca gtgagatcaa caaggcggcc acgatgccga tgatcaagct gaggatggcg
     6061 gctgaggtcg cggcggat
//
LOCUS       DASVTM010000493         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1003370,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000493 DASVTM010000000
VERSION     DASVTM010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003370"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..710)
                     /locus_tag="VF633_13650"
     CDS             complement(123..710)
                     /locus_tag="VF633_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX8472150.1"
                     /translation="MSAGKKRAGLIAIICGGTVVAMTGAAFAAVPLYKMFCQATGFDG
                     TVRKAEKAPDQILDRMVTIRFDTNVRDLPMKFTAEQNTQRVRIGETGIAWFDVTNTSD
                     KPIQARAAYNVVPEYAGPYFQKLQCFCFDSQTIEAGETRKFPVQYFVAPELATDREAR
                     GATEITLSYTFYPSKDGAPEALGDGQTGLAIPARS"
     gene            complement(707..937)
                     /locus_tag="VF633_13655"
     CDS             complement(707..937)
                     /locus_tag="VF633_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472151.1"
                     /translation="MVVQLTAVELAARKRRNVWIALALCAFIMLLFSTTFLRMQANQK
                     AEQAKAAALAAGPMHPERTYDSKMAPSPGTVQ"
     gene            complement(950..>1776)
                     /locus_tag="VF633_13660"
     CDS             complement(950..>1776)
                     /locus_tag="VF633_13660"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX8472152.1"
                     /translation="AIAILCIAVGAGAAGALNMAIEGETDALMRRTRGRPVAAGRINK
                     NDAMAFGAVLSLFSVMLLGMNTNWLAGGLLALTIVYYAGFYTIMLKRRTPQNIVIGGA
                     AGALPPVIGWAAATGSAPWQAWLLFAIIFLWTPPHSWALALYSAGDYAKAGIPMMPVA
                     RGAKSTRLQILIYSLVFVPVAIAPGLVGLGGWIYLAVSILGGLGFLGLAWRLYRSRAG
                     DEPDKADTVGREAAIYDVKLAAKPARNLFAFSILYLLGLFAALLVENLSNLSPVSF"
BASE COUNT          332 a          547 c          602 g          295 t
ORIGIN      
        1 tcggccatcg tcagtatctc gtggagaagt cgaaagtcgt tgtcaggcta tagcccattt
       61 ttccggtgcg tcgagcgacg gcacggcaaa aatgaggctc gacctcggat cggcgctgcg
      121 gtttagctgc gcgccgggat cgccagaccg gtctggccgt ctccgagcgc ctcaggagcg
      181 ccgtcctttg acggatagaa ggtgtagctg agcgtgatct cggtggcgcc gcgcgcctca
      241 cgatcggtgg ccagctcggg ggcgacgaaa tactggaccg ggaatttccg cgtctcgccc
      301 gcctcgatcg tctggctgtc gaagcagaag cattgcagct tctggaaata cggtccggcg
      361 tattccggca cgacgttgta ggccgcgcgg gcctggatcg gcttgtcgga cgtgttggtg
      421 acgtcgaacc aggcaatgcc ggtctcgccg atccgcaccc gctgggtgtt ctgctcggcg
      481 gtgaacttca tcggcaggtc gcggacgttg gtgtcgaagc ggatggtgac catccggtcg
      541 aggatctggt ccggagcctt ttcagccttg cgcaccgtgc cgtcgaagcc tgtcgcctga
      601 cagaacatct tgtagagcgg gacggcggcg aaggcggccc cggtcatggc gacgacggtt
      661 ccgccgcaga tgatggcgat caggcccgcg cgtttcttgc ccgcactcat tgtactgtcc
      721 ccggagacgg cgccatcttc gaatcatacg tgcgctcggg atgcatcggc ccggccgcca
      781 aagccgcagc cttggcctgt tcggccttct ggttagcctg catcctcagg aaggtggtgc
      841 tgaacaggag catgatgaag gcgcacagag cgagcgcgat ccagacgttg cggcgcttgc
      901 gggccgccag ttcaacggct gtaagttgga cgaccatcag cgaaaaaccc tagaacgaaa
      961 caggcgacag gttggacagg ttctcgacca gcaaggcggc gaacagtccc aacagataca
     1021 ggatcgagaa ggcgaacagg ttgcgtgcgg gcttggccgc cagcttgacg tcgtagatcg
     1081 cggcttcgcg acccacggtg tcggccttgt caggctcatc gccggcgcgt gaacgataca
     1141 ggcgccaggc cagaccgagg aagccgagac cgccgaggat cgagaccgcc agatagatcc
     1201 agccgccgag accgacgagg ccgggagcaa tggcgaccgg cacgaacacc agcgaataga
     1261 tcaggatctg cagtcgggtc gactttgcac cccgggccac cggcatcatc ggaatacccg
     1321 ccttggcgta gtcgccggcc gaatagaggg cgagagccca gctatgcggc ggggtccaca
     1381 ggaagatgat ggcgaacagg agccaggcct gccacggggc cgagcccgtc gccgccgccc
     1441 agccgatcac tggcggaagg gcgcccgccg cgccgcctat gacgatgttc tggggcgtcc
     1501 gacgcttcag catgatggtg tagaaaccgg cgtagtagac gatggtcagg gccagcagcc
     1561 cgccggccag ccagttggtg ttcatgccca gcagcatgac cgagaacaac gacagcacgg
     1621 ccccgaaggc cattgcgtcg ttcttgttga tgcgacctgc cgcgacggga cgaccgcgcg
     1681 tgcggcgcat cagggcgtcg gtctcgccct cgatcgccat attcaaggcg cctgcggcgc
     1741 ccgcgccgac agcgatgcac aggatggcga tggccg
//
LOCUS       DASVTM010000494         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_417198,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000494 DASVTM010000000
VERSION     DASVTM010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417198"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VF633_13665"
     CDS             complement(<1..290)
                     /locus_tag="VF633_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8472153.1"
                     /translation="MTTQVELALRGPEAFQLARSALTEMESAGVWPTPLNFELWLHYV
                     NDPEGPLGREITRLMATKDAFTEGTAEMLAAEYLPRGRLSEEIRDAGAVLNR"
     gene            complement(412..>1981)
                     /locus_tag="VF633_13670"
     CDS             complement(412..>1981)
                     /locus_tag="VF633_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8472154.1"
                     /translation="LAGRDVLGIAQTGTGKTAAFTLPLIDKLSKGRARARMPRALVLA
                     PTRELADQVAMSFEKYAKGTKLSWVLLIGGVSMGEQVAALNKGVDVLIATPGRLLALF
                     ERGKMMLNGVEIMVIDEADRMLDMGFIPDIERIFKLTPPKRQTLFFSATMPPEITRLT
                     TQFLKDPTRIEASRPAMTAETITQYLVRIPSADPKAKRAALRVLVGRDDVKNGIVFCN
                     RKSEVDVVAKSLKQHGFDAAPIHGDLDQSLRTKTLADFRSGALKILVASDVAARGLDI
                     PDVSHVFNYDVSHHADDYVHRIGRTGRAGKTGQAFMLVTPADDKGLDKVLKLIKMAPE
                     ELKLDIDWSNLSGAAPRREAPARETSARDGAGREGSGREGSCQEKPEGRSRGRARRSE
                     SSSEAIAPIEASTVITPAAPVVAPADTDEAPARRTRSRRTRGGEKTIVAEPVLADVRG
                     ATVVEAPVQAPPEATKAEPAPAAEPRQSREPQLRQADRPGASKDRELPPAREGAGFGS
                     DIPAFLRRPVPARG"
BASE COUNT          329 a          613 c          675 g          364 t
ORIGIN      
        1 tcgcggttca ggacagcgcc cgcgtcgcgg atttcttcag acaaacggcc acggggcagg
       61 tattcggcag ccagcatctc ggccgtgcct tcggtgaagg cgtccttggt cgccatcaga
      121 cgcgtaatct cgcggccgag cggtccctcg gggtcattga cgtaatggag ccagagctcg
      181 aaattgagtg gagtcggcca gacgcctgcg gactccattt cggtcagggc gctacgggca
      241 agctgaaaag cttccggtcc tctaagcgct agttcgacct gggttgtcat cgctatcccc
      301 cgatacgtct tctgacgccg acggagagta cgttaaccgc ggttccgtaa cgacgggtta
      361 agcggcaagg aaaaaccccg ccactcaacc tgaggtcata cggacgcgtg actacccgcg
      421 agcaggaacc gggcggcgca ggaaggcggg tatatcggag ccgaagcctg caccttcacg
      481 agccggcggc agttcgcggt ccttcgaggc acccggacga tcggcctgac gcagttgcgg
      541 ttcacggctt tgacgaggtt cagcggcggg cgccggttca gcctttgtgg cttcaggcgg
      601 tgcttgcaca ggcgcttcta cgacagttgc tccgcgaacg tcagcgagga ccggctctgc
      661 aacgatggtc ttctcgccac cgcgtgtacg gcggctgcgc gtgcgccggg caggggcttc
      721 atccgtgtcg gccggggcaa caacaggtgc ggcgggcgtg atgaccgtcg aggcttcgat
      781 gggcgcgatg gcctccgaag aggattcaga gcggcgggcg cggccacggc tccggccttc
      841 cggtttttcc tggcaggaac cttcgcggcc cgaaccttcg cggcctgcac cgtcgcgagc
      901 ggatgtctca cgagccggcg cctcacgacg aggcgcagcg ccactgaggt tcgaccagtc
      961 gatgtccagc ttgagttctt cgggagccat cttgatcagc ttgaggacct tatccagccc
     1021 cttgtcgtcg gccggggtca ccagcatgaa ggcctgaccc gtcttgcccg cgcgaccagt
     1081 ccggccgatg cggtggacgt agtcgtcggc gtggtgcgag acgtcgtagt tgaacacgtg
     1141 gctgacgtcg gggatgtcga gaccgcgtgc ggcgacatcc gacgccacca ggatcttcag
     1201 cgcgcccgag cggaaatccg ccagagtctt ggtgcgcagc gactggtcca gatcgccatg
     1261 gatcggcgcg gcgtcgaagc cgtgctgctt cagcgacttg gcgaccacgt cgacttcaga
     1321 cttgcggttg cagaagacga tgccgttctt gacgtcgtca cgaccgacca gaacccgcag
     1381 cgcagcgcgc ttggctttcg ggtccgcgct cggaatgcgg accagatatt gcgtgatggt
     1441 ctcggccgtc atggcggggc gcgaggcctc gatccgggtc gggtccttga ggaactgggt
     1501 cgtcagccgg gtgatctcgg gcggcatcgt cgccgagaag aacaacgtct ggcgcttggg
     1561 cggcgtcagc ttgaagatgc gttcgatgtc cgggatgaag cccatgtcga gcatccggtc
     1621 ggcttcgtcg atgaccatga tctcgacgcc gttcagcatc atcttgccgc gttcgaacag
     1681 ggccagcaga cggcccggtg tagcgatcag gacgtcgacg cccttgttca gcgcagcgac
     1741 ctgctcgccc atcgaaacgc cgccgatcag cagcacccag ctcagcttgg tgcccttggc
     1801 atatttctcg aagctcatcg caacctggtc ggccagttcg cgcgtcggag ccagaacgag
     1861 ggcgcgcggc attcttgccc gggcgcggcc cttggacagt ttgtcgatga gggggagggt
     1921 gaaggcagcg gtcttgccgg tgcccgtctg ggcgatgccg agcacgtcgc gtccggcgag
     1981 g
//
LOCUS       DASVTM010000495         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_686395,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000495 DASVTM010000000
VERSION     DASVTM010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686395"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /gene="trxA"
                     /locus_tag="VF633_13675"
     CDS             complement(<1..465)
                     /gene="trxA"
                     /locus_tag="VF633_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX8472155.1"
                     /translation="MSLIGETQTKPASDLIKEGTDAGFMADVIEASRSVPVIVDFWAT
                     WCGPCRQLTPTLEKAVLAADGKVKLVKIDVDKNPAYAGQLRVQSIPTVYAFVNGQPVD
                     GFQGAQPESQIKAFIDRLSKTGAGADGAGEVEALLALAAESAAVGDLGGAAQA"
     gene            complement(501..1055)
                     /locus_tag="VF633_13680"
     CDS             complement(501..1055)
                     /locus_tag="VF633_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX8472156.1"
                     /translation="MKDRAALFAHLEAHGIGHSTLDHPAVFRVEEGLEIKAALPGGHT
                     KNLFLKDKKDQLWLISALGETTIDLKKLPAAIGSGRLSFGSADLMQQTLGVTPGSVTA
                     LGLINDTQHRIRFVLDAALAGADPVNFHPLTNEATTALSQAGFAAFLASVGVTPIIVD
                     FETLTIIDGPSRGCGSAALRPSLP"
     gene            1122..>1621
                     /locus_tag="VF633_13685"
     CDS             1122..>1621
                     /locus_tag="VF633_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEX8472157.1"
                     /translation="MELECYPTSNRLPEIVAGRQSRAWMDHFMERHPYRCLPLSMANT
                     SGWELLCPMGFTAEWDGNVYQESIKLTPDVPHPDFHEFAKSHFSHGVLTLHAGYLFKT
                     PPGWSMWVMGSPNHIKDGIQALSGLVETDWLPFPFTMNWLFTRPGKIRFEKNEPFCFI
                     TLTQDKV"
BASE COUNT          284 a          519 c          531 g          287 t
ORIGIN      
        1 ggcctgggcg gcgccgccga ggtcgcccac ggcggcggac tccgccgcca gagccagcag
       61 ggcttcaacc tcgccagccc cgtcggcgcc cgcgccggtc ttggacaggc ggtcgatgaa
      121 ggccttgatc tggctttcgg gctgcgcccc ctggaatccg tcgacgggct ggccgttcac
      181 gaaggcgtag acggtgggga tcgactgcac gcgcagttgg ccggcgtagg ccggattctt
      241 gtcgacatcg atcttgacca gcttgacctt gccgtcggcg gccagcacag ccttttccaa
      301 ggtgggggtc agctgacggc aggggccgca ccaggtcgcc cagaagtcaa cgatgaccgg
      361 aacgctccgc gacgcctcga tcacgtcggc catgaagccg gcgtcagtgc cctccttgat
      421 caggtcggaa gcgggcttgg tttgggtttc gccgatcagg ctcatgggcg gtgacaactc
      481 ctcagggcga ttgaacgcgt tcatggcaaa gatggtcgca aggctgccga gccgcaaccg
      541 cgcgagggcc cgtctatgat cgtcagggtc tcgaaatcga cgatgatggg cgtcacgccg
      601 acgctggcga gaaaggcggc aaaaccggcc tggctcagcg ctgtggtggc ctcattggtc
      661 aggggatgga aattgaccgg atcggcgccc gccagcgcgg cgtcaagcac gaagcggatc
      721 cgatgctgcg tgtcgttgat cagccccagg gctgtgaccg agcccggcgt cacgcccagc
      781 gtctgctgca tgaggtcggc cgagccgaac gacagccgac ccgatccgat cgcagccggc
      841 agtttcttga gatcgatcgt ggtctcgccg agcgccgaga tcagccagag ctggtccttt
      901 ttgtctttga gaaacaggtt cttggtatgg ccgcccggaa gcgccgcctt gatctccaga
      961 ccctcttcga cgcggaacac ggccgggtgg tcgagcgtcg aatggccgat cccgtgagct
     1021 tcgagatggg cgaagagggc ggcgcggtct ttcatcttgc cggttctcta gcgtcccgcg
     1081 cgcgcgaagg ttagaggatt tgcagcgccg cggagagacc catggaactg gaatgctacc
     1141 cgaccagcaa ccggctgccg gaaatcgtcg ccggccggca gtcgcgggcc tggatggacc
     1201 atttcatgga gcgccatccc tatcgctgcc tgccgctcag catggccaac acctcgggct
     1261 gggagctgct ctgtccgatg ggcttcacgg ccgagtggga cggcaacgtc taccaggaat
     1321 cgatcaagct cacccccgac gtgccgcacc ccgatttcca tgaattcgcc aagtcgcact
     1381 tcagccacgg ggtgctgacg ctgcacgcgg gctatctctt caagacgccg ccaggctggt
     1441 cgatgtgggt gatgggctcg cccaaccata tcaaggacgg catccaggcc ctgagcggcc
     1501 tggtcgagac cgactggctg cccttcccct tcaccatgaa ctggctgttc acgcgcccag
     1561 gcaagatccg cttcgaaaag aacgagccgt tctgtttcat caccctgact caggacaagg
     1621 t
//
LOCUS       DASVTM010000496         6895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_417330,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000496 DASVTM010000000
VERSION     DASVTM010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417330"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VF633_13690"
     CDS             complement(<1..799)
                     /locus_tag="VF633_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8472158.1"
                     /translation="MTHPLNVALVGYGFAGKTFHSPLISACPDLQLHTVVSTRPDDVL
                     ADWPDAQVVHDLPTALADLEIDLVVIATPDALHAEQAEAALTAGKAVVVDKPFALSLA
                     DARRVVALAEERNLFLSVFQNRRWDADFLALKAEIASGRLGRIVTYESHFDRYRPVVR
                     DRWREGPGGGVWQDLGPHLIDQALVLFGRPLGITCDLAIQRDGGLAPDWAHAVLRYQH
                     IRVVLNAAMLIAAPDVRFAVHGTKGSWLTSGLDPQEDQLKSGMPVGSA"
     gene            complement(944..1990)
                     /locus_tag="VF633_13695"
     CDS             complement(944..1990)
                     /locus_tag="VF633_13695"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8472159.1"
                     /translation="MNAHAIIKPGTPGLLTPSAAYKPFRYPWAFDMWKTQQQVHWMPE
                     EVPLGEDMKDWAVNLNDKERNLLTQIFRFFTQSDIEVADNYMEKYGRVFKPTEVKMML
                     ASFANMETIHIAAYALLLETIGMPESEFGAFMEYEAMRAKHDYMAQFGVDSEADIART
                     LAMFGGFTEGLQLFASFAMLMNFPRQNKMKGMGQIVSWSVRDESLHCDGIIKLYHAFN
                     AETGVVTKAVADDIVQCCETVVGLEDKFIDLAFEAGDVNGMTPDDIKQYIRFIADWRL
                     RQLKLPELYGIKENPLPWLQSLLSGVEHANFFEARATEYSKAATQGSWHGSEGVWGEF
                     DKMMARRDMSMIAG"
     gene            2032..2625
                     /locus_tag="VF633_13700"
     CDS             2032..2625
                     /locus_tag="VF633_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX8472160.1"
                     /translation="MRSDRPGAKSQSGYVAITFVRHAISSVFIQRLADWLGIILPTHL
                     ASDPPVFPFARRRVRIARRFLFSPGKTMKLIVRVTNSIGELLLLYLAVIVVCAAAYAM
                     LEHKPFFDAVWWAVVTATTTGYGDMYPATLGGRLIAVLLMHATLLFILPLLIGRIIGT
                     MIEDQHRFTDEEQRQLLADIAEIKAALAARSAGGDPI"
     gene            2622..3086
                     /locus_tag="VF633_13705"
     CDS             2622..3086
                     /locus_tag="VF633_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8472161.1"
                     /translation="MTKHCYDWPRPAVTTDVAVFADRDGEASILLIQRGGEPFAGCWA
                     LPGGFLEEDETLEVCATRELKEETGLEAGPLRLFGTYSTPGRDPRGRTVTVAYWTRAE
                     EGSGEATAGDDAADCRWFALNRLPTLAFDHDRIIADAVRSLTYAASASSSSD"
     gene            complement(3053..5053)
                     /locus_tag="VF633_13710"
     CDS             complement(3053..5053)
                     /locus_tag="VF633_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472162.1"
                     /translation="MLAAALQLHRRQAIRVLAVCFALNILGNLVRGDPQPFLWLNAVL
                     NLGEACLAALIARRVCGAALDLRRPRRLANFALLAVVPAVLLSAFAILSVAGPMRGYT
                     TPIYLFTLHRFFDMETLGLLVVAPALLLIAKSHRFRSTAPASPLETSVVVSLLFVVTA
                     AVFFQSITPAAFAIFPFLLLVSFRTSPSWVAGSIMGVAIISGVATVTGHGPVQMTQLP
                     PLPGLEQVPPLLRQLTIYYVFLLGVIVTALPVSTAMSERRRLIERLERRTLSAHNARL
                     RAEEADAAKSRFLALMSHEMRTPLNSVTGYAEVLARRPDLDPVAVEQLANIQRSGDAL
                     LLLVEDVLEISRGDNALCLAPLSLMDVIAAAASPSRITADDKGLSLVVDVRPDALPEM
                     IGDRRRLRQALHHLISNAVKFTDRGTITVRADRINDEVLIAVTDTGCGLDMAGVDRLF
                     GAFVQGDDSISRAHIGAGIGLTLVRRHAGLMGGSVVVDSRPGDGATFTLRLPLALAEI
                     GASSTLATGAEAALANDIDEGDDARCARVLVVDDHPANREVARLMLTAIGCEVTEATD
                     GEEAIQMAATQVFDLILMDVRMPRVDGLAATRAIRAAGNGVPILAVTADAMPEDAARC
                     LAAGMDDHLAKPISHHALYSAMERLMSGQRRVQSELEDAEAA"
     gene            5344..6132
                     /locus_tag="VF633_13715"
     CDS             5344..6132
                     /locus_tag="VF633_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472163.1"
                     /translation="MSDTITDPTFHPPADALDFYEESLRLLKESGIPFLLSGTYAVTA
                     YTGIRRPTKDLDVFCKPGDYPRILAYFQARGYRTDVEDERWIAKVWKDEKHFFDVIFA
                     MSNGTIAVNDAWFGEDTIEVYGHEVAITPPTALILSKVFIQDRYRYDGADVNHIILKQ
                     SDAIDWKSLLDQMDLYWEVLTAHLLNFRFAYPTERGRVPAWLMTELTGRLAAQVNLPA
                     PRVKVCRGRLFSARDYVLDISEWGFGDVVGKGLDETHDPVNLGH"
     gene            6144..>6895
                     /locus_tag="VF633_13720"
     CDS             6144..>6895
                     /locus_tag="VF633_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8472164.1"
                     /translation="MSDASPISSDPQTPQPGMDSGPLRKLRVAAVGDLHVGENHDRPH
                     RALFERVHEDADVLCLCGDLTNFGKTREVEILLEDLRACKIPMVGVLGNHEHECGQPE
                     HVTKMLCDAGVRMLTGQAFEIEGVGFAGGKGFVGGFGRYMLSSFGESSIKTFVQEAVE
                     DANAIENSIRTLRTERSVVLLHYSPVVDTVMGEPPEIHAFLGSSRLAETIDRYDNISL
                     VVHGHAHRGAPEGRTNKGTPVYNVALPVLRTLG"
BASE COUNT         1292 a         2048 c         2332 g         1223 t
ORIGIN      
        1 ctgcagagcc gacgggcatt ccggatttga gctgatcttc ttgcgggtcg aggcccgagg
       61 tcagccagct gcctttcgtg ccgtgaaccg cgaagcggac atcgggtgcg gcgatcagca
      121 tggcggcgtt gaggacaacg cggatgtgtt ggtagcgcag gaccgcatgg gcccagtcgg
      181 gagcgaggcc gccgtcgcgc tggatggcca gatcacaggt gatgccgagc ggtcgaccga
      241 acagaaccag cgcctgatcg atcaggtggg ggccgaggtc ttgccagaca ccgccgccgg
      301 gaccctcgcg ccagcggtcg cggaccaccg gacgatagcg gtcgaaatgg ctctcgtagg
      361 tgacgatgcg accgaggcga cccgaggcga tctcggcttt caaggcgagg aagtcggcgt
      421 cccaacggcg attctggaac acggaaagga acaggttgcg ctcctcggcg agggcaacga
      481 cccggcgggc gtcggcgagg ctgagggcga agggcttgtc gactacgacg gccttgcccg
      541 ccgtcagggc ggcctcggcc tgttccgcgt ggagggcgtc cggggtggcg atgacgacga
      601 ggtcgatctc gaggtcggcc agagccgtgg gcaagtcatg gacgacttgc gcgtccggcc
      661 agtcggcgag gacgtcgtct ggccgggtgg agacgactgt gtggagctgg aggtcgggac
      721 acgccgatat cagcgggctg tggaaggtct tgccggcgaa gccgtagccg acgagagcga
      781 cgttaagagg atgggtcatg caaaatcacc gctcatcccg cgaaagcggg gcccagtccc
      841 ttgatgcaca acgaacacat cagattcgtc cgctgacatc gttacctgtg tcgccaaagt
      901 gctgggtccc cgctttcgcg gggatgagcg gagtttggag agatcagccc gcgatcatgc
      961 tcatgtcgcg gcgggccatc atcttgtcga actcgcccca gacgccttcg ctgccgtgcc
     1021 aggaaccctg ggtggcggct ttggagtatt cggtggcgcg ggcttcgaag aagttggcgt
     1081 gttcgacgcc cgacagcaac gactgcagcc acggcagcgg gttttccttg atgccgtaga
     1141 gttccggcag cttgagctgg cgcaggcgcc agtcggcgat gaagcggatg tattgtttga
     1201 tgtcgtcggg cgtcatgccg ttcacgtcgc cggcctcgaa ggccagatcg atgaacttgt
     1261 cttcaaggcc cacgacggtc tcgcagcatt ggacgatgtc gtcggcgaca gctttggtga
     1321 cgacgccggt ctcggcgttg aaggcgtggt acagcttgat gatgccgtcg cagtgcaggc
     1381 tctcgtcgcg cacggaccag ctgacgatct gccccatgcc cttcatcttg ttctggcgcg
     1441 ggaaattcat cagcatcgcg aaggatgcga acagctgcag cccttcggtg aagccgccga
     1501 acatggcgag cgtgcgggcg atgtccgcct cggagtccac gccgaactgg gccatgtagt
     1561 catgcttggc ccgcatggcc tcgtattcca tgaaggcgcc gaactcgctc tcgggcatgc
     1621 cgatggtttc gagcagcagg gcgtaggccg cgatatggat ggtctccatg ttcgcgaacg
     1681 aggcgagcat catcttgacc tcggtcggtt tgaacacccg accgtatttt tccatatagt
     1741 tgtccgcgac ctcgatgtcc gactgggtga agaagcggaa gatctgcgtc agcagattgc
     1801 gctccttgtc gttcaggttg acggcccagt ccttcatgtc ctcgccgagg ggcacttctt
     1861 cgggcatcca gtggacctgc tgctgggtct tccacatgtc gaacgcccac ggataacgga
     1921 acggcttgta ggctgccgag ggggtcagaa ggccgggagt gccgggcttg atgatggcgt
     1981 gggcgttcat ctgaggagag ctttcgaacg attccgtgga ctgcattcac catgcggtcg
     2041 gatcggcccg gcgccaagag ccaaagcggc tatgttgcga tcacttttgt acgacacgct
     2101 atatctagcg tatttatcca gaggttagcg gattggctgg ggatcatttt gcccacgcat
     2161 ctcgcgtccg acccgccagt ctttcccttc gcgcggcggc gcgtcaggat tgcgagacgt
     2221 tttttgttct ctccgggaaa gaccatgaag ctgatcgttc gcgtcaccaa cagcatcggt
     2281 gagctcctgc tgctgtatct ggcggtgatt gtcgtgtgcg ccgcggccta tgccatgctg
     2341 gagcacaaac cctttttcga tgcagtatgg tgggcggtgg tgacggctac gaccaccgga
     2401 tatggcgata tgtatccggc gacactgggt ggtcggttga tcgcagtcct gctgatgcat
     2461 gcaacgttgt tgttcatcct gccgctgctg attggccgga ttatcggaac gatgatcgag
     2521 gaccagcaca gattcaccga tgaggaacag cgccagctgc tggccgacat tgccgagatc
     2581 aaggcggcgc tggctgcgcg ttcggccggc ggagatccga tatgacaaag cactgctacg
     2641 actggccgcg tccggcggtg acgaccgacg tggcggtctt cgcggaccgt gacggggagg
     2701 cgtcgatcct gctcatccag cggggcggag agccattcgc cgggtgttgg gcgctgccgg
     2761 gtgggttcct cgaggaggac gagacgctgg aggtttgcgc gacgcgcgag ctgaaggagg
     2821 agacaggtct cgaggccggc ccgttacgcc tgttcggaac ctacagcaca cccgggcgtg
     2881 atccccgtgg acggaccgtg acggtcgcct actggacgcg agcggaggag ggttcgggag
     2941 aggcgacggc gggagatgat gcggcggact gccggtggtt cgctttgaac cggttgccaa
     3001 cgctggcctt cgaccatgac aggatcatcg ccgatgccgt gagaagcctg acctacgcgg
     3061 cttcggcgtc ctcgagttca gattgaaccc ggcgttggcc agacatcaga cgttccatcg
     3121 ccgaatacag ggcgtggtgc gagatcggct tggcgagatg gtcgtccata cctgcggcga
     3181 ggcagcgtgc cgcatcctcg ggcatggcgt cagccgtgac ggccaggatc ggcacgccat
     3241 tgccggcagc gcggatggcg cgggtcgcgg cgaggccgtc aacgcgcggc atgcgtacat
     3301 ccatcaggat caggtcgaaa acctgcgtgg cggccatctg gatggcttct tcgccatctg
     3361 tggcctcagt gacctcgcaa ccgatcgccg taagcatcaa ccgggcgacc tcgcgattcg
     3421 cgggatggtc atcgacgacc agaacgcggg cgcatcgcgc atcatcgcct tcgtcaatgt
     3481 cgttcgcgag agccgcttca gcgccagtgg ccagagtgct cgaggcgccg atttcggcca
     3541 gggccagggg cagacgcagg gtgaaagtgg cgccgtcgcc gggtcggctg tcgaccacca
     3601 ccgaaccccc cataagtccc gcatggcgcc gaaccagggt caggccgata ccagcgccga
     3661 tatgagcgcg gctaatggag tcgtctccct ggacgaaggc gccgaatagc cggtcgacgc
     3721 cggccatatc gagcccgcaa ccggtgtcgg tgaccgcaat cagaacttcg tcgttgatgc
     3781 ggtcagcccg aacggtgatc gtcccgcgat cagtgaactt cacggcgttc gagatcaggt
     3841 gatgcaaggc ctggcgcaga cgtcggcggt ccccgatcat ctcgggaaga gcgtcgggcc
     3901 ggacgtcgac aacgagggac aggcccttgt cgtccgcagt gatccggctc ggcgaggctg
     3961 cggcggcgat gacgtccatc agggacaggg gagccagaca cagggcgttg tcgccacgcg
     4021 aaatctccag cacatcctcc accagcaaca gcagggcgtc gcccgagcgc tggatattgg
     4081 cgagctgctc cacggccacg gggtccagat cggggcgacg ggcgagaacc tcggcataac
     4141 cggtgacgct gttcaagggg gtgcgcatct cgtggctcat caaggccagg aagcgggatt
     4201 tggcggcgtc ggcctcctct gcgcgcaggc gggcattgtg ggcggagagg gtgcgacgct
     4261 cgagccgttc gatcagacga cggcgctcgg acatcgcagt tgagaccggc agggcagtca
     4321 cgatgacccc gagcagaaag acgtaataga tcgttagctg gcgcagcagc ggcgggacct
     4381 gctcaagccc cggcagaggc ggaagctggg tcatctgcac gggaccatgg ccggtgaccg
     4441 ttgcgacgcc ggagatgatc gctactccca tgatcgagcc cgctacccag gacggcgagg
     4501 tgcggaacga taccagcagc aggaagggga aaatcgcgaa agcggcaggc gtgatcgact
     4561 ggaagaagac ggcggcagtc acgacgaaca gaagcgacac gacgaccgag gtctccagag
     4621 gcgaggccgg ggccgtcgag cggaacctgt gcgacttggc gatcaacaac agggctggtg
     4681 cgactaccag cagaccgagg gtttccatgt cgaagaagcg gtgcagggtg aacaggtaaa
     4741 tcggcgtggt gtatccgcgc attggcccag ccacactgag gatggcgaat gcagagagca
     4801 gtacggctgg aaccaccgcc agcagcgcga aattggccag acgccttggt cgccgcaggt
     4861 cgagggcagc gccgcagacc cggcgcgcga tcagggcggc cagacaggcc tcgccgagat
     4921 tcagcaccgc gttcagccac aggaagggct gcggatcacc gcgtaccagg ttgccgagga
     4981 tattgagcgc gaaacaaacc gccagaacgc ggatagcctg acgtctgtgc aactgcaggg
     5041 cggcggcgag catgactccg ttagccggcc atataactac cgcctgaaac gtccgcgcgc
     5101 tccagatccc caccagtaga caggcggcga acaggaccac atacgcccag gccggtggcc
     5161 ctgaggcctg tcccgcatcg tgtctgaaca gtcgccaacg accgcgcatt tcggcccctc
     5221 cggctgcgaa ttaaactgtg atcgcacgca gaggttaaat ttcaggcgcc aacggtgtca
     5281 tgcgtcgcag gaacctgcgc caccgccgat ccgttgctca acctcgtccg aggaggggcc
     5341 gccgtgtcag acaccatcac cgatccgacc tttcatccgc cggcggatgc actcgacttc
     5401 tatgaagaga gtttgcgctt gctcaaggag agcgggattc cctttctgct gtcgggcact
     5461 tatgcggtca ctgcctatac tggcatccgg cgaccgacca aggatctgga tgtcttctgc
     5521 aagccgggcg attatccccg cattctcgcg tatttccagg cgcgcggtta ccggaccgac
     5581 gtcgaggacg agcgctggat cgccaaggtc tggaaggacg agaagcattt cttcgacgtg
     5641 atcttcgcca tgtccaacgg gaccatcgcc gtcaacgacg cctggttcgg cgaggatacg
     5701 atcgaggtct atggccatga ggtcgcgatc acaccgccta cggccctgat cctgtcgaag
     5761 gtcttcatcc aggatcgcta ccgctacgac ggggcggatg tgaaccatat catcctgaaa
     5821 cagtcggacg cgatcgactg gaagagcctg ctggatcaga tggacctcta ttgggaggtg
     5881 ctgaccgcgc accttctgaa ttttcgcttc gcctatccga ccgagcgagg tcgggtccct
     5941 gcctggctga tgacggagct gaccggacga ctggcggccc aggtcaacct gccggcgccc
     6001 cgcgtgaagg tttgccgggg gcggctgttc agcgcccgcg actatgtcct cgacatctcg
     6061 gagtggggtt tcggcgacgt ggtcggcaag ggcctcgatg agacccacga tccggtcaac
     6121 ctcggccatt gaaggaaatc ggcatgagcg acgccagccc catatcgtcc gacccccaaa
     6181 cgccccagcc cggcatggac tcgggtccgc tcaggaagct gcgcgtggcg gccgtcggcg
     6241 acctgcatgt cggagagaac cacgaccgac ctcaccgggc cctcttcgaa agggtgcacg
     6301 aggacgccga tgttctgtgc ctgtgcggag acctgaccaa tttcggcaaa acgcgcgagg
     6361 tcgagattct gctggaggac cttcgggcct gcaagatccc gatggtcggc gtgctgggca
     6421 atcacgagca cgaatgcggc cagccggagc atgtgacgaa gatgctgtgc gacgccgggg
     6481 tcaggatgct gaccggccag gcctttgaga tcgagggcgt cggttttgca ggcggcaagg
     6541 gcttcgtcgg cggctttggt cgctacatgc tgagttcttt cggcgagagc tcgatcaaga
     6601 ccttcgtgca ggaagccgtc gaggacgcca acgcgatcga gaactcgatc cgtaccctgc
     6661 gcaccgaacg ctcggtggtg ctgctgcact attccccggt ggtcgacact gtgatgggtg
     6721 agccacccga gatccacgcc ttcctggggt cgtcgcggct ggccgagacc atcgaccgtt
     6781 atgacaacat cagtctggtg gtgcatggcc acgcccaccg aggagcgccg gaggggcgga
     6841 ctaacaaggg tacaccggtc tacaatgtcg ccctgccggt tctgcggacg cttgg
//
LOCUS       DASVTM010000497         8235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_794603,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000497 DASVTM010000000
VERSION     DASVTM010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794603"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5372)
                     /locus_tag="VF633_13725"
     CDS             complement(<1..5372)
                     /locus_tag="VF633_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472165.1"
                     /translation="MTKAGAKGGFEFLGARASNAGDEFHELWATRAAIRLLDRNDDLQ
                     ALAVEGVAAVDVPADDVRVWDGVDCTLYFGGSSAADARHIRIEQLKYSAAAPNQPWTV
                     SRLVSGDTRQKTVLNKLARGWAELRAKQPTGATLETALVSNQPIDDGLRTALKTLGAR
                     PIKPSRTRPPDTADDVAWLAWAIDLDPADCKTFLASFCPEGGAGSRFAVEERALQAVA
                     LWSDEGVLGRLSDLRRYVRGRMGPQPGGEVISAETVIFQIGGVEYTALFPCPPDLAST
                     ANPIPRPEIDQALGDLTSGRVRRLCLHGEAGVGKTTALAQVGAGLPAGSEMVVYDCYG
                     GGRYLDSDALRHRGTDAFMQLTNELAARLNLPLLVVRRTMTDPVRVFANRLRHAAGVL
                     AAVNPNAFLVIAVDAADNAIAAGRAKGEPPFIHEFLDLGDLPDNVRLVVSARTGRLPD
                     LSLPRSWTPTPLGPFELPQTTALVARQFAVSDAWLRDFHDLTHGIPRVQSYALRSHSA
                     DEALDRLLPAGKSLGDVFVGLFEDALKKSGAAADITRICAGLIALPRPIPVATLAEVI
                     GVAEAHLIDFCGDLAPALRVRQDVISFADEDFEAFVRQEGVAELDAVREKLANHLLVR
                     AETDRYAAEHIASALSDAGRRLELLDLVEGEPAPRVIIDPVVRREVELKRLRLAIQAC
                     GATQDRARALKFVLIGAEGQRSEKALAQTLRDNLDLSVRFASGPLGRVLLSEGGKQRD
                     HISVFYQRLLHAADRGDTVSGRESQKLIQAYWENHRTSVRKTSGVTDTDILAHVDSAS
                     RLGGAAAAIQAARSWQPRGTAILLAAELPSRLVAAGRSSEVEALLADPDLPSSLAPLL
                     AAPLLLKGFKADPDLSDTLASILPWLRLRDFYRSNAAEVTRHGVVLDLVLCCCELSAG
                     NAACRPAIDAILKAILRPEFRGIDARSAWEAQKLDLLFRAWALSERLAGREPVMRSLF
                     LPRPVPSSPPEPAGRKVRQAEKDARDKARQAEESHDASLHGMVDVFSDLYRLRADVFV
                     RPSDAEALEENALGEALTRVVVKVQQSEWRLGREHGTPVLRALAARSLLALMGLGASS
                     DAIVDAALRIHGGDDEGLPNKSFVERLSLWPEQHVRLVRSLGALAQDRRDARIGAEDK
                     SRMLMAIARYLLPLSADDAGAVFNHAIEAAAELDNEIVQQLELIDATVTRAGAVIVEP
                     GRLANELSEVATDAGIRLQDLDHFPWAEVSRALAVLDPVQALAACARWEDADLGVRDR
                     TLGPVVLSALQSGRMRPEQAAALGHLLDQPDEALTASLETVGGPLVEEMAWDRLIRGQ
                     GRGERIVATPAGPWSEALVAQQAFLNRLTPGMQPDPEAEPPDRTGSGAGADAAPWPAA
                     VLTDAAALGDEVLARMEADRAQERYGGADRHLTDARAAATADRVGHLEALARLAGPRL
                     GRAVAHALLGALTDWRGLPAVDGWRRAHLGQVIADRLPELTRYVRFPDQPLDDLLELA
                     ELDDAAVVETLLKGIEIHVGELDGESLFGLAHRAAVRLPPAAAASLTDWFIGRLYHRI
                     PLKDREAVPPDAIPATVDEAVARYLFAVMGDIDVRLRWRAGHALRRLARLGDTASLDE
                     HLKQFARREEPAFRYHDGPFYWLAAKQWAVMTWTRIASERPQVGVRARDALFDTATDP
                     ALPHLLIQLMARDGCLALSTMGVVTWTAQEANAIQAVGVTPHPRKHISQKRGGLGRYE
                     RRDGRKVRRFDFDGLDTLPYWYDPVLRCFATLKPEALLEEAERWILDVWGHAPEPWAL
                     DRTRRRT"
     gene            5823..7727
                     /locus_tag="VF633_13730"
     CDS             5823..7727
                     /locus_tag="VF633_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8472166.1"
                     /translation="MELSSVSVTGFRRFKKTATLQTNGKLVALLGPNEAGKSSLLAAI
                     VLLANDLPPAAQDYTRGVAKTHFALVGRYFLDDHDLEAAGLVGNYWLHITKGQEGTRN
                     YALYPNPPSRDRGPRTRLLSAIDALVAHPKFLKSLSDDEKSKALGDVADVRAILTSDA
                     ENISAENLQVVANLANSFEIRVTDKLPAPLQKLSEHSARFAETEAEPDPNAKAMAALL
                     PRVPGVVSFDEGARTLVGDYALDELRQKIPPALDNLADLAEVSVKDIIAAHDRGSSDL
                     MTIQARANAQLEKRFREDWKQSGISVSVRVGDGRLEIHIVNEGSQYTPFAERSDGLRL
                     FVALAAFTGGKWAGKPVLLIDEAEQRLHYDAQADLVQMLARQTVASKVIYTTHSAGCL
                     PEDLGMGVRLTRPDRADPTLSEIENRFWASDPEGFTPLLFGMGAATMAFFPTRHAVMV
                     EGPADMLLMPTMIRAALDRASLGVQFVPGLSSTADFQQSPVIGTAKGMLYLVDGDAGG
                     DSIKTRLVKNGVRIDDIIVVKSPNGNAAELEDFLHVSLLVDGANRLLSRFHPESSMIT
                     QADLGVTARMAALEKAFRSKSGKTLSKVNFAYSVLDLLLDNPKRTLFDARTKPAFVNL
                     AAKTLNYFRL"
     gene            8005..>8235
                     /locus_tag="VF633_13735"
     CDS             8005..>8235
                     /locus_tag="VF633_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8472167.1"
                     /translation="MKIAALTIENFRGIRQARVRFGTHTVFVGPNNCGKTTIIEALAL
                     LLGRDRMVRTLTEHDFYGSNPQPADRIKLVVTI"
BASE COUNT         1531 a         2655 c         2597 g         1452 t
ORIGIN      
        1 gttcgccgac gtgtccggtc cagtgcccaa ggctcgggcg catggcccca gacatcgagg
       61 atccaccgct ccgcttcctc tagcagcgct tcgggcttga gtgtggcgaa gcaccggagc
      121 acgggatcgt accagtaggg caaggtgtcc aagccgtcga agtcgaaccg ccggaccttg
      181 cgtccatccc gtcgctcgta acggcccagt cctccccgct tctgcgagat gtgttttcga
      241 gggtgcggcg tcacgccgac ggcctggatc gcgttagcct cctgagccgt ccaggtcacc
      301 acccccatgg tcgacagggc gagacagccg tcccgggcca tgagctggat aagcaggtga
      361 ggcagtgccg ggtccgtcgc agtgtcgaac agggcgtccc gggcgcggac gccgacctgc
      421 gggcgttcgg aggcgatccg ggtccatgtc attaccgccc actgcttcgc cgcgagccag
      481 taaaaggggc cgtcgtggta tcggaacgcc ggctcttccc gccgggcgaa ctgtttgagg
      541 tgttcgtcga gactcgcggt gtcgccgagg cgcgccagcc gacggagcgc atgcccggcg
      601 cgccagcgca ggcgaacgtc gatgtctccc atgacggcga agagatagcg cgcgaccgcc
      661 tcatccaccg tggcggggat ggcgtctggc gggacggcct cccgatcctt cagtgggata
      721 cggtggtaaa gccgtccaat gaaccagtcg gtcagactgg cggcggcggc cggcggcaag
      781 cggaccgccg cccgatgcgc cagtccgaac agggactccc cgtccaactc ccccacatgg
      841 atctcgatgc ctttgagcag ggtctcgacg acggcggcgt cgtccagctc ggccagctcc
      901 agcaaatcat cgagcggctg atcgggaaac cggacgtagc gcgtgagctc cggaaggcgg
      961 tcggcgatca cctggcccaa gtgcgcgcga cgccagccgt cgacggcggg cagacctcgc
     1021 cagtcggtca gcgctccaag cagggcgtga gcgaccgcgc gtcccagccg gggcccggcc
     1081 aagcgcgcca aggcctccaa gtgccccacc cggtcagcgg tggccgccgc ccgcgcgtcg
     1141 gttaggtgac ggtcggcccc cccgtaacgc tcttgtgccc ggtccgcctc cattcgcgcg
     1201 agaacttcgt cgcctagggc ggcagcgtcc gttaggacgg ccgcaggcca aggcgcggcg
     1261 tcggcgcccg cccccgagcc ggtcctgtcc ggtggctcgg cctccgggtc gggctgcatt
     1321 ccaggcgtca ggcggttgag gaaggcctgc tgggcgacga gggcctcgga ccagggacct
     1381 gcaggggtcg cgacgatccg ttcgcccctg ccctgccctc gtatcaggcg atcccaggcc
     1441 atttcctcaa cgagggggcc acccacggtc tcaaggctcg ccgtcagcgc ttcatccggc
     1501 tgatcaagga gatgaccgag cgccgcggcc tgttcggggc gcatccggcc tgactgaagg
     1561 gctgacagca ccaccggtcc caaggtgcgg tcgcgcaccc cgaggtccgc gtcctcccag
     1621 cgcgcgcagg cggccaaggc ctgcacggga tctaggacgg cgagcgcgcg tgagacctcc
     1681 gcccaaggga agtgatcaag atcctgcagc cgaatccccg cgtcggtcgc gacctccgac
     1741 agctcgttgg ccagccggcc gggctctacg atcaccgccc cggcgcgcgt gacggtcgcg
     1801 tcgatcagct ccagctgctg gacgatttca ttgtccagtt ccgccgcggc ctcgatcgca
     1861 tgattgaaaa ccgccccggc gtcgtctgcg ctcaggggca gcagataccg cgcgatagcc
     1921 atcagcatcc tggacttgtc ctccgcgccg atccgggcgt cgcggcggtc ctgggcgagt
     1981 gcgccgagac ttcgcaccaa gcgaacgtgc tgctctggcc acaggctgag acgctccacg
     2041 aagcttttgt tcggcagacc ttcgtcatcg ccgccatgga tcctaagggc ggcgtccacg
     2101 atcgcgtcgg acgacgcgcc aaggcccatc aatgcgagca gggatctcgc tgccaaggcc
     2161 ctgagaaccg gcgtcccgtg ttcgcgtccc agacgccatt ccgactgctg gaccttgacg
     2221 acgacgcgcg tcagcgcctc gcccaaagcg ttctcttcca gcgcctcggc gtccgacggt
     2281 cgcacgaaaa cgtcggcgcg aagccgataa aggtctgaga agacatcgac catcccatga
     2341 agcgatgcgt cgtggctttc ttccgcctgc cgcgccttgt cccgggcgtc cttttcggcc
     2401 tgccggactt tccgcccggc cggctccggc ggcgacgatg ggaccggtcg cggcaggaac
     2461 agtgaccgca tcacaggttc acggcccgcc agacgctcgg acaaggccca ggcccggaac
     2521 agcagatcca gcttctgcgc ctcccaggcg gaccgcgcat cgatcccccg gaactcggga
     2581 cgaaggatgg ccttgagaat ggcgtcgatc gcgggtcgac aggcggcatt cccggccgag
     2641 agttcgcagc agcacaacac caggtccaag accaccccgt gccgggtcac ctcggcggcg
     2701 ttgcttctgt agaaatcgcg gagccgaagc catggcagga tgctagctag ggtgtccgat
     2761 agatccggat cggccttgaa gcccttcagg aggagcggcg cggcgagcag tggcgccagg
     2821 cttgagggga ggtctgggtc cgcgagaagg gcctcgacct ctgatgaccg cccggcagcg
     2881 acaaggcggg acggcagttc ggcggcgagg aggatggcgg tccctctcgg ctgccaggac
     2941 cgcgcggcct gaatcgccgc cgcagcgccg ccaaggcgtg aggcggaatc gacatgggcc
     3001 aggatatccg tatcggtcac gccgctcgtc tttcgaacgg acgtcctgtg attctcccaa
     3061 taggcctgga tgagcttctg gctctcccga cccgacactg tgtcgccacg gtccgcagcg
     3121 tggagcaggc gctgatagaa gacagagatg tggtcacgtt gcttgccgcc ctcggacagg
     3181 agcactcgtc caagggggcc actcgcgaac cggaccgaaa ggtcgagatt atcgcgtagc
     3241 gtctgggcga gcgccttctc cgagcgctgg ccctcggccc cgatgagcac gaacttcagc
     3301 gcacgggcgc gatcctgggt cgcgccgcag gcctggatcg ccagccgcaa ccgtttcagc
     3361 tcgacctccc gccgcacgac cgggtctatg atcacacggg gtgcgggttc gccctcgacc
     3421 agatccagaa gttcaagtcg ccgcccggcg tcactcaagg ccgaggcaat gtgttcggcc
     3481 gcgtagcgat cggtttccgc ccggacgaga aggtggttcg caagtttctc gcgaaccgcg
     3541 tccagctccg ccacgccttc ctggcggaca aatgcttcga aatcctcgtc cgcgaaactg
     3601 atcacgtcct gccgcacccg caacgcaggt gccagatcgc cgcaaaaatc gatcagatgc
     3661 gcctcggcga cgccaatgac ctcggccagg gtggccacgg ggatggggcg tggcagggcg
     3721 atcaagcctg cgcagatccg ggtgatgtcg gcggccgctc cactcttctt gagcgcgtcc
     3781 tcgaataggc cgacgaacac gtcgccgagg gattttccgg cgggcagaag tcggtccaga
     3841 gcctcgtccg cgctgtgaga ccgcagggca tagctctgga cgcgcggaat gccatgggtg
     3901 agatcgtgaa aatccctgag ccaggcgtca gataccgcga actgccgggc gaccaaggcc
     3961 gtcgtctgcg gtagctcgaa cggacctagc ggagtcggcg tccagctccg tggcagcgac
     4021 aggtcgggca atcgccccgt ccgcgccgac accaccagac gcacattgtc gggcaggtcc
     4081 ccgaggtcga gaaactcgtg aatgaagggc ggctcgccct tcgcccgccc tgcggcgatg
     4141 gcgttgtcgg cggcgtccac ggcgatgacc agaaaggcat tggggttgac ggccgccagc
     4201 acgcccgccg catgccgcag ccgattggcg aagaccctga cgggatcggt catcgtcctc
     4261 cgaacgacca acagagggag gttcagtcgg gcggccagct cattggtgag ctgcataaag
     4321 gcgtcggtcc cgcgatgtcg cagggcgtcg gaatcgagat aacgacctcc gccataacag
     4381 tcataaacta ccatctcgga tcccgccggc agcccggccc caacctgggc cagcgcggtc
     4441 gtcttgccga ccccggcctc gccgtggagg cagagccggc gcacgcgccc tgatgtaagg
     4501 tcgccgagcg cctgatcgat ttcaggccga gggattggat tggccgtcga ggcgaggtcc
     4561 gggggacagg ggaagagcgc cgtatattcc acgcccccga tctggaagat gacggtctcc
     4621 gcgctgatga cttcgccgcc gggctgcgga cccattcttc cccggacgta gcggcgaaga
     4681 tccgacagtc gcccgaggac accctcatcg ctccaaagcg ccaccgcttg gagggcgcgt
     4741 tcctcgaccg cgaaccggga tccggcgccg ccctcggggc agaaggacgc caggaaggtc
     4801 ttgcaatcag ctggatcgag atcgatcgcc caggccaacc aggcgacgtc gtcggccgtg
     4861 tcgggcggcc gtgtccgact gggcttgatg ggccgggcgc ccagtgtctt gagcgcggtc
     4921 cgcaagccgt catcgatcgg ctgattgctg accagcgccg tctcgagcgt ggcgcccgtc
     4981 ggctgcttcg cccggagttc ggcccatccg cgcgcaagct tgttcaggac cgtcttctgg
     5041 cgcgtatcgc cggacacgag gcgtgacacc gtccacggct ggttgggggc cgcggccgag
     5101 tatttgagct gttcgatccg gatgtgtcgg gcgtcggcgg cggacgaccc gccaaaataa
     5161 agggtgcagt ccacgccgtc ccagacgcgc acatcatctg ccggcacatc cactgccgcg
     5221 acgccctcca ccgccagggc ctgaagatcg tcgttgcgat cgagcagccg gatcgccgcc
     5281 cgggtcgccc acagttcgtg aaactcgtcg ccggcgttag atgcgcgcgc gcccagaaac
     5341 tcgaaaccgc ccttcgcccc cgccttcgtc acgctgtcat gccttcgctc gaatcactcc
     5401 gcgcctaggt tgcagcagga tggcgaggtc gggaagttcc gctgccgaca ggagcgacga
     5461 tggccgacac accgcaacat ccaatcggcg acggttcagg atcgcctgaa tcgcctacat
     5521 ctgacggagt cgaagaagtc cgcgagcccg tcctccggac gcaccgcgca cctagctcaa
     5581 cgaacgatcc ttctgaatgt cggctaagag tcacaaacgg actttgccct gccccctcat
     5641 tgcatttgtc ttcattcaca ctgagtccgc ttctgacccg tgcgacgttt ccgtttatgg
     5701 cgatcagaac tagggctttg gagtgtagtg aggcggagcc gctcccgctc cggcagcaac
     5761 agctggcttg accgaccgga tctatgtcac tcaactggca ccttcatgag gggtgatgcc
     5821 aaatggaact gagttcagtt tccgtgaccg gatttaggcg atttaaaaag acagcgacgc
     5881 ttcagaccaa cgggaagttg gtcgcgctgc taggtcccaa tgaagctgga aaatcttccc
     5941 tactcgccgc aatcgttctt ctcgccaacg atctgccgcc cgccgcgcag gattacacac
     6001 gtggcgttgc gaaaactcat tttgcgttgg tcggccgata ctttctcgac gatcatgacc
     6061 ttgaagcggc agggttggtg ggaaactatt ggctacatat caccaagggc caagaaggca
     6121 ctcggaatta cgccttgtat ccaaacccgc cgagcagaga tcgaggaccg aggacgagac
     6181 tactcagtgc catcgacgct ctggtcgctc atccgaagtt tctcaagtca cttagcgacg
     6241 acgagaaatc aaaggcgctc ggagatgtcg cagacgtccg tgctattctg actagcgacg
     6301 ccgaaaacat ttccgccgaa aatctgcaag tggtggcgaa tcttgcgaat agcttcgaaa
     6361 tccgggtcac cgacaagctt ccggcacccc ttcagaaact ctcggagcat tccgctcggt
     6421 ttgccgagac agaggccgag ccagatccca acgccaaggc gatggctgct ttgttgccgc
     6481 gagttccggg tgtcgtctcg ttcgatgaag gtgcgcggac gttagtaggg gactacgccc
     6541 tggacgagtt gaggcaaaag atcccccccg ccctagataa tcttgccgat ctcgcagagg
     6601 tgagcgtcaa ggatatcatt gcagcacatg accggggcag ctcggacctc atgactatcc
     6661 aagcgcgcgc gaacgcgcag cttgagaagc gcttccgcga agattggaag cagtctggaa
     6721 tttccgtgtc ggtgagagtc ggggatggcc gtctcgaaat ccacattgtc aacgagggga
     6781 gccagtacac tccgttcgcc gagcgaagtg acgggcttcg tttattcgtc gcgcttgcag
     6841 cgtttacggg cggaaaatgg gcgggaaaac cggtgctgct catcgatgaa gcagagcagc
     6901 gtcttcacta cgacgcccaa gcggatctgg ttcagatgct cgctagacag accgtcgcgt
     6961 cgaaggtcat ctacacaacg cactcggcag gatgtttgcc agaggacctc ggcatggggg
     7021 tgcgactgac taggcccgat cgagcggacc caaccttgtc cgaaatcgag aaccgctttt
     7081 gggccagcga cccggaaggg ttcacgccac ttctcttcgg catgggagcg gccacgatgg
     7141 ccttcttccc tactcgacat gctgtgatgg tcgaaggtcc ggcggatatg cttctgatgc
     7201 cgaccatgat ccgtgcagcg ctcgatcgtg catcgcttgg ggtacagttc gtacctggtc
     7261 tctcgtcaac cgccgacttc cagcagtcac cggtcatcgg aaccgcgaaa ggaatgctct
     7321 atctggtcga cggcgatgcc ggcggggact cgattaagac gcgcttagtc aaaaacgggg
     7381 ttcgaataga tgacatcatc gtggtgaagt cgccaaatgg gaatgcggct gagcttgagg
     7441 acttcctgca cgtaagcttg ttggtcgatg gggccaatcg actattgtcc cgcttccacc
     7501 cggaatcctc aatgattacc caagccgatc tcggcgtgac tgcgcgcatg gcagcgctgg
     7561 agaaagcctt taggtcgaaa tctggaaaga ccctctcgaa agtcaacttc gcttactcag
     7621 ttttggactt gctattggac aaccccaagc gaactctgtt tgacgctagg acgaagcctg
     7681 cgtttgtgaa cttggccgcc aagaccctca actattttag actttgaacg agcatccaga
     7741 gcaaatctga tcgttgagtc ttctgcggtc tctctaagtc ggctcttggc gcttagccga
     7801 ccacaaaggc tgtgagaact cgctcctact cagtgggatt ttcgcaatct gcactcactg
     7861 acctttcccg acgctctagg cgtcggcaag gtgcgcactt gagcgccctt gtctgagacg
     7921 tgcttgcgga ctgcttgcca agtagagcag cggcggttga gcatatgcgc agtcctgcta
     7981 tggttgccta gccaaggggg gcgcatgaag attgcagcct taactatcga gaattttaga
     8041 ggcattcgac aggcgcgagt gcggtttggc acgcacactg tgttcgttgg accgaataac
     8101 tgcggtaaaa caacgataat cgaagctcta gctttgctcc ttggacgcga ccgcatggtt
     8161 cgcacgctta ccgagcacga tttctacggt agcaatcccc agccggctga ccgcataaag
     8221 ttagtcgtca cgatc
//
LOCUS       DASVTM010000498         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_740571,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000498 DASVTM010000000
VERSION     DASVTM010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740571"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VF633_13740"
     CDS             complement(<1..1410)
                     /locus_tag="VF633_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX8472168.1"
                     /translation="MAMIETILLLLLAVVVSGAVARMTRLALPLVQIALGAVVVLITG
                     HTIDLKPDIFFLLFLPPLLFLDGWRIPKEDLFRDRAVILELALGLVLFTVLGLGFLIY
                     WMIPEMPLPVAFALAAILSPTDPIAVKAIAARVPMPKRLMHILEGESMLNDATGLTCM
                     RIAVLAATTGAFSIDHAVGTFAWLAIVGVLIGIAVTLAITYGKGFISRKWGEDVGAQI
                     LISLLIPFAAYLVAEEVHASGILAAVAAGITMSFTERGDGQSLAMTRIRRAVVWDTVQ
                     FVANGVIFVILGEQMPSIFARAAEVVANTTRPETWWLAVYVFGIVAALAAIRFIWVWT
                     SLKLTLYRRRHRGPPPKVGLRLTMVMSLAGVRGAITLAGILTLPFVLADGSPMPSRSL
                     AIFLAAGVIIVSLVVATVALPRLMKNIELPPEPSHQQEEDRSRVAAATAALQAIEDKA
                     HALAEGKLDPDLYADVASRI"
     gene            complement(1415..>2479)
                     /gene="hflX"
                     /locus_tag="VF633_13745"
     CDS             complement(1415..>2479)
                     /gene="hflX"
                     /locus_tag="VF633_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX8472169.1"
                     /translation="AVVVDDQLSPVQQRNLEKEWDCKVIDRTGLILEIFGRRARTKEG
                     RLQVELARLDYEKSRLVRTWTHLERQRGGTGSTGGPGETQIELDRRLIADRIVKLKGE
                     LEEVRRTRGLHRKQRQKAPFPTIALVGYTNAGKSTLFNRLTGSEVLAKDLLFATLDTT
                     QRTIRLPQGRPAIVADTVGFISDLPHELVESFRATLEEVGEADLILHVRDIASLDSAA
                     QAKDVETVLKQIEIPEGKTRRVLEIWNKIDLLDDEARDNILGLAERLATEGKAVAVSA
                     WTGERIEPLRHTITTLIDDDPETELTLAPSQGDALAWLYQHGRVTSRDTDTEGHTHVV
                     VRLAPQALGRFERLYPDTMA"
BASE COUNT          444 a          753 c          886 g          396 t
ORIGIN      
        1 gattcggctc gcaacgtcgg cgtagaggtc ggggtccagc ttgccctcgg ccagggcgtg
       61 ggccttgtcc tctatggcct gaagggcggc ggtagcggcc gcgacgcggg acctgtcctc
      121 ttcttgctgg tgcgacggtt cgggcggcag ttcgatgttc ttcatcaggc gcggaagggc
      181 cacggtcgcc accaccagcg agacgatgat caccccggca gcgaggaaga tcgccagact
      241 acgcgacggc atcggcgatc cgtcggcgag cacgaacggc agcgtcagga tgccggccag
      301 ggtgatggcc ccgcgaacgc ccgccagcga catgaccatg gtcagacgga gaccgacctt
      361 gggcggcgga ccgcgatggc ggcggcgata aagggtcagc ttcagcgagg tccacaccca
      421 gatgaaccgg atggcggcaa gggcggcgac gatgccgaag acatagacgg ccagccacca
      481 ggtctccgga cgggtagtgt tggcgaccac ctcggcggcg cgggcgaaga tcgacggcat
      541 ctgctcgccg aggatgacga agatcacgcc gttggcgacg aactgaaccg tgtcccagac
      601 tacggcgcgg cggatgcggg tcatggccag agactggccg tcgccacgct cggtgaagct
      661 catggtgatg ccggcggcga cggcggcgag aatgcccgac gcatggacct cttcggccac
      721 cagataagcg gcgaacggga tcaacaggct gatcaggatc tgggccccaa cgtcctcacc
      781 ccatttacgg ctgatgaagc ccttgccgta ggtgatggcc agagtgaccg cgatgccgat
      841 aaggacaccg acgatggcca gccaggcgaa ggtgccgacc gcgtgatcga tggagaaggc
      901 gccggtggtg gcggccagaa cggcgatccg catgcaggtc aggccagtgg cgtcgttcag
      961 catcgattcg ccctcgagga tatgcatcag ccgcttgggc atgggcacac gggcagcgat
     1021 cgccttgacc gcgatcggat cggtcggcga caggatggcg gcgagcgcaa acgcgaccgg
     1081 cagcggcatc tccgggatca tccagtagat cagaaagccg aggccgagga cggtgaacag
     1141 caccagcccc agcgccagct caaggatcac cgcccggtcg cgaaacaggt cttccttggg
     1201 gatacgccag ccatcgagga acagcagcgg cggcaggaac agcaggaaga agatgtccgg
     1261 cttcaggtcg atggtgtgac ctgtgatcaa gaccacgacc gcccccagcg cgatctgcac
     1321 cagaggcagg gcgagtcggg tcatccgcgc cacggccccc gagaccacca cggccagcag
     1381 cagcagcagg atggtctcga tcatggccat gagatcaggc catggtgtcg gggtacaggc
     1441 gctcgaaacg gcccaacgcc tggggcgcga ggcggaccac gacgtgggtg tggccttcgg
     1501 tgtcggtgtc gcgcgacgtt acgcgtccgt gttggtagag ccaggccaga gcgtcacctt
     1561 gcgaaggggc aagcgtcagc tctgtctccg gatcatcgtc gatcagggtc gtgatggtgt
     1621 ggcgcagggg ttcgatcctc tccccggtcc aggccgagac cgcgacggcc ttgccctcgg
     1681 tcgccagccg ttcggccaga ccgaggatgt tgtcgcgggc ctcgtcgtcg aggaggtcga
     1741 tcttgttcca gatttcgagc acgcggcgag tcttgccctc cgggatctcg atctgtttga
     1801 gcacggtctc gacgtccttg gcctgggcgg cgctgtcgag agaggcgatg tcgcggacgt
     1861 gcaggatgag gtcggcctca cccacttctt cgagggtcgc gcggaagctc tcgaccagtt
     1921 cgtgcggcag gtcggagatg aagccaacgg tgtcggcgac gatggccgga cgaccttgag
     1981 gcaggcgaat ggtgcgctgg gtcgtgtcga gcgtggcgaa caacaggtcc ttggcgagga
     2041 cctccgaccc ggtcagccgg ttgaacagcg tcgacttgcc ggcgttggtg tagccgacga
     2101 gggcaatggt cgggaacggg gctttctggc gctgtttgcg gtgcagacca cgcgtacggc
     2161 gcacctcctc cagctcgccc ttcaacttga cgatgcggtc ggcgatcagg cggcggtcga
     2221 gttcgatctg ggtctcgccg ggaccgccgg tcgagccggt gccgccacgc tgacgctcaa
     2281 ggtgggtcca ggtgcggacg aggcgggatt tctcgtagtc gaggcgggcc agttcgacct
     2341 gcaaccgccc ttccttggtg cgggcgcgtc gaccgaagat ctcgaggatc aggccagtgc
     2401 ggtcgatgac cttgcagtcc cattcctttt ccagattgcg ctgctggacg gggctcagct
     2461 ggtcgtcgac gacgacggc
//
LOCUS       DASVTM010000499         5314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_152389,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000499 DASVTM010000000
VERSION     DASVTM010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152389"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF633_13750"
     CDS             complement(<1..511)
                     /locus_tag="VF633_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8472170.1"
                     /translation="MDLKLTGKRALICGGSSGLGRAVATALVREGVHVALLSRDAEKL
                     QPIADELNAMGPGRAVIAPADMADHASLLAAVDVAEKALGGPIEILLNNTGGPPPSGV
                     VGLDPDLWRAQFEQMVLSVFRLTDRVLPAMRSASWGRILNVASVSLIEPIGTLGVSNT
                     LRASIAAWAK"
     gene            complement(517..1926)
                     /gene="lpdA"
                     /locus_tag="VF633_13755"
     CDS             complement(517..1926)
                     /gene="lpdA"
                     /locus_tag="VF633_13755"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8472171.1"
                     /translation="MAEATAQYDVVIIGGGPGGYNAAIRAGQLGLKVACVEMRETLGG
                     TCLNVGCMPSKALLHASELFEAAVKEFPTLGIEISPPKVNLPQMMKQKGESVTALTKG
                     IEFLFKKNKADWIKGRGKLAGPGKVEVTAADGTVTTLGAKNIIIATGSEPTPLPGVDF
                     VEGKVVDSTGALSLAAIPNKLIVVGAGIIGLELGSVWRRLGAEVTVVEYLPRITPGMD
                     TDLATAFQRSLTKQGITFKLGSKVTAAKTTKTGVELTVEPAAGGAAETLSGDVVLVAI
                     GRRPYTAGLGLETVSVETDKRGFLVNDHFKVAEGVWVIGDVTTGPMLAHKAEEDAVAA
                     VENIAGKAGHVDYNLVPSVVYTSPEVAWVGRTEDQLKADGVAYKVGKFPFTANSRAKI
                     NHETEGFAKVLADATTDKILGVHIMGPQAGEMIGEACIAMAFGGASEDIARTSHAHPT
                     RSEAVKQAAMGVENWTMQA"
     gene            complement(2057..4009)
                     /locus_tag="VF633_13760"
     CDS             complement(2057..4009)
                     /locus_tag="VF633_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8472172.1"
                     /translation="MLLEGEVDWVLVLSVIAGVMTVLALFSSWFAYTHRERHVSTSGE
                     LNHVQKVVAETDKRLFETLNAIPVAIVQTDMQGKFVFANKAAHQLMGRRDAELLGLRF
                     HSATWGITFPDGRPIPPDMLPSARALRGQTVRGFQHILANPATRRKMLVCVTAMPIEN
                     EYGQIIGSTAAVVETEGLTTPEAAIAPERVLPHPDTDLTGRVFESASSRLVVVSPHGV
                     VRVANAAALRQLDRTDSIVGDDFADQFLAEDQRLEARHILRAALTAKTGLGTTTGQFD
                     RIFARGAGGATVVWRILPLLNSDGVIDALLLAGDELDAEIEPRPAGDSEVAAADALEY
                     QGTPELTPEPMEAGADTSEAARDHAHGLQAVEIETHPGAHGSTDSAGLDALRDERDAA
                     VSHAEEALSQLHAARAEAETAMAEADRISARARAEDDRARRFEDVGRLSSGMAQDFNA
                     LISVMTSALDMMLKQADDPSRVRRLGQAALAAGQRGETMTRRLAALSVEETGGARTLD
                     CGVLLRAMEGRLRSLAGPGIDLMIEGPSSSVPVRIDPVAFEATVRALVTNAVEAVGGA
                     GSVAVRLETTSIGARLSVRDTGPGMDGDTIPRAAEPFFTTKSGAAGMGLAQARAFARQ
                     SGGALTIDSTPGEGAEVAVTLPPDAD"
     gene            complement(4221..>5314)
                     /locus_tag="VF633_13765"
     CDS             complement(4221..>5314)
                     /locus_tag="VF633_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8472173.1"
                     /translation="RDTTILEAEGEAEDVTAFRILTAAEFEDFEIVDGGLNTLEETRI
                     DPYVMLSGETGQLKWQAGLRYETTDVEITDETVADDLRTTGSDYGILLPSASLRLALS
                     DTDRITVAVARTVRRPNFDNLSPVKLEEEHGDNDFRGNPFLEPETAWGLDVGFERRLG
                     RRGVVGINAFYRDVTDLIEDINTGEQGSGGAGTFIESIDNVGDGQVYGVEFDLSTPLD
                     FIGMEQTGVFLNASWLDSKVDDVLGERRFNDQSDYVLNVGFTHDIKTWGAAFGATYRK
                     QGDAFGRVYAEEVRTSYGADLEIFVEKQLFANTVLRLTGSNLLNSSKDETFDKFDDQN
                     DQLGRDYDEYELETESAGPVYQLVARVAF"
BASE COUNT          920 a         1713 c         1725 g          956 t
ORIGIN      
        1 tcttggccca ggcggcgatc gaggcgcgga gcgtattgga cacgcccagc gtcccgatgg
       61 gctcgatcag cgagaccgag gcgacgttga ggatacggcc ccagcttgca gagcgcatcg
      121 cgggcagaac ccggtcggtc aggcggaaca cggacagcac catctgctcg aactgcgccc
      181 gccacaggtc cgggtcgagc ccgaccacgc ccgacggggg aggcccgccg gtgttgttga
      241 gcaggatttc gatcggtccg ccgagcgcct tctcggccac atcgactgcc gccagcagcg
      301 aggcatgatc ggccatgtcg gcgggagcga tgaccgccct gcccggtccc atggcgttca
      361 actcgtcagc gatgggctga agcttttcgg catcgcgcga caacagggcg acgtgcacgc
      421 cctcccgcac cagagcagtg gcgaccgcgc gaccgaggcc gctggagccg ccacagatca
      481 aggcgcgttt gccggtaagc ttcaggtcca tcggctttag gcctgcatgg tccagttttc
      541 cacgcccatg gccgcctgtt tgacggcttc ggagcgtgtg gggtgggcgt ggctggtgcg
      601 ggcgatgtct tcggaggcgc cgccgaaggc catggcgata cacgcctcgc cgatcatttc
      661 gccggcttgc ggacccatga tgtggacgcc cagaatcttg tccgttgtcg catcggccag
      721 gaccttggcg aagccttcgg tctcgtggtt gatcttggcg cggctgttgg cggtgaaggg
      781 gaatttgccg accttgtagg cgacgccgtc ggccttgagc tggtcctcgg tcctgccgac
      841 ccaggccact tccggcgagg tgtagaccac gctcggcacc agattgtagt ccacatggcc
      901 ggccttgccc gcgatgttct cgacggctgc gacagcgtct tcttcggcct tgtgcgccag
      961 catcggtccg gtcgtgacgt cgccgatgac ccagacgcct tccgctacct tgaagtggtc
     1021 gttgaccagg aagccgcgct tgtcggtctc gacgctaacg gtctcaagac ccagacccgc
     1081 cgtgtacgga cgacggccga tggcgaccag aacgacatcg ccgctcaggg tctcggccgc
     1141 accacccgca gcaggctcga ctgtcaactc gacgccggtc ttggtcgtct tcgcagcggt
     1201 caccttggag ccgagcttga aggtgatgcc ttgtttggtc agtgagcgct ggaaggctgt
     1261 ggccagatcg gtgtccatgc cgggcgtgat gcgcggcaga tattcgacga ccgttacctc
     1321 ggcgcccaga cgacgccaga ccgagccgag ctccagcccg atgatgcctg cgccgacgac
     1381 gatcagcttg ttggggatgg cggcgagcga cagggcgccg gtggagtcga cgaccttgcc
     1441 ctcgacgaaa tcgacgcccg gcagcggggt gggctcggag ccggtggcga tgatgatgtt
     1501 cttggcgccc agggtcgtga ccgtgccgtc ggctgcggtg acctcgactt taccgggacc
     1561 ggcgagcttg ccccggccct tgatccagtc ggccttgttt ttcttgaaca ggaactcgat
     1621 gcccttggtc agggcggtga cgctctcacc cttctgcttc atcatctgag gcaggttgac
     1681 cttgggcggg ctgatctcga tgccgagagt cgggaactcc ttcaccgccg cctcgaacag
     1741 ctccgaggcg tgcagaaggg ccttcgaggg catgcagccc acgttcaggc aggtgccgcc
     1801 gagagtttcg cgcatctcga cgcaggcgac cttcaggccc agctgaccgg cgcggatcgc
     1861 ggcgttatag ccaccgggac cgccgccgat gatgacgacg tcgtattggg cggtggcttc
     1921 ggccatgaga tcctcggggt ctggtttttc gggcgtggga gcgcgcgagc tttaactagc
     1981 cgctgaccgc cgggggtcaa ccaccacgca gaatatagga cgccggaaac cggatcgcta
     2041 acggtcgaaa gcggaatcag tccgcgtcgg gcggcagggt caccgcgacc tcggcgcctt
     2101 cgccgggcgt gctgtcgatc gtcagggctc cgccggattg acgcgcaaag gcacgcgcct
     2161 gagccaggcc catgccggcg gctccggact tggtggtgaa gaaaggctcg gcggcccggg
     2221 ggatcgtgtc gccgtccatg cccgggccgg tatctcgcac cgagagacga gcgccgatgg
     2281 acgtggtctc cagccgtacg gcgacggaac cagcgccgcc gacggcttcg accgcattgg
     2341 tgaccagagc ccggactgta gcttcgaagg ccaccggatc gatccggacg ggaacggacg
     2401 aggacggccc ctcgatcatc aggtctatgc caggtccagc gagcgaccgc agacggccct
     2461 ccatcgcgcg cagaaggaca ccgcaatcaa gtgtacgggc gccgcccgtt tcctcgactg
     2521 acaaggccgc aaggcgacgc gtcatggtct cgccgcgctg gccggcggcg agcgcagcct
     2581 gccccagacg gcgaacccgc gagggatcgt cagcctgttt cagcatcatg tcgagcgcgc
     2641 tggtcataac gctgatcaag gcattgaaat cttgcgccat gccgctgctc aggcgaccta
     2701 cgtcctcgaa acgccgggcc ctgtcgtctt cggctctggc gcgagcagaa atacgatcgg
     2761 cttcggccat ggccgtctca gcctcggcgc gcgcggcgtg gagctgcgac agagcctctt
     2821 cggcatggct aacggcggcg tcacgctcat cgcgcagggc atccaaccca gcggaatcgg
     2881 tcgatccatg ggcgcccgga tgggtttcga tctcgaccgc ttgcaggccg tgggcgtgat
     2941 ctctggcggc ctcggacgtg tcggccccgg cttccatagg ctccggcgta agctccggcg
     3001 tgccctgata ttcgagggcg tctgccgccg ctacttcgga atcgcccgcg ggccggggct
     3061 cgatttcagc gtcgagttcg tcccccgcca gaagcagggc atcgatcact ccgtccgaat
     3121 tgagcagggg cagaatgcgc cagactacgg tcgcaccccc ggccccccga gcaaaaatcc
     3181 ggtcgaattg ccccgtggta gtgccgagtc ccgtcttcgc cgtgagcgct gcgcgcagga
     3241 tgtggcgtgc ctcgagccgc tgatcctctg caaggaactg atccgcaaaa tcgtcaccga
     3301 cgatactatc ggtccggtcc agttgacgca gcgcagcggc gttagcaacc cgcacgacac
     3361 cgtgtggcga gacgaccacc agccggcttg aagccgattc aaagaccctg ccggtgagat
     3421 cggtgtccgg atgaggcaag accctctcgg gtgcaatcgc ggcctcgggc gtggtcaatc
     3481 cctcggtctc cacgacggct gcggtcgaac cgatgatctg cccatactca ttctcgatcg
     3541 gcatggccgt tacacagacc agcatctttc gccgcgtcgc agggttggcg agaatgtgct
     3601 ggaagccccg gacagtctga ccccttagcg cgcgggcgct gggcagcatg tcaggcggta
     3661 tcggccggcc gtcgggaaag gtgatgcccc aggtcgcgga atggaaacgc agccccagaa
     3721 gctcggcgtc gcgtcgtccc atcaactggt gagccgcctt gttggcgaag acgaacttgc
     3781 cctgcatgtc ggtctggacg atggcgactg gaatggcgtt cagggtctcg aacaggcgct
     3841 tgtcggtctc agccacaacc ttctgtacgt ggttgagttc gccggaggtg ctaacgtgac
     3901 gctcccggtg ggtgtaggcg aaccaactgg aaaagagggc gaggacggtc atgacgcccg
     3961 cgatcacgct cagcaccaac acccagtcga cctcgccttc gagcagcatc tgtcaatccc
     4021 attccggcgc caggaaacgc cgcacccatc cagactgcaa gggacgcgcc gtcctggcgc
     4081 ctgctcgcga gccggcccgc cgccgtggcg cacaggccat caacttcagt catcatctta
     4141 cgcccgacgg cgaacaccag atatgaaaaa gggccggacg tccccgcccg gccccgtttc
     4201 ggatcggaag aagcgtcgcc ctagaatgcg acgcgagcta ccagttggta aacgggccca
     4261 gcggactccg tttccaattc gtactcgtcg tagtcgcgac cgagctgatc attctggtcg
     4321 tcgaacttgt cgaaggtctc gtccttgctg gaattcagca ggttggaccc cgtcaggcgg
     4381 agaaccgtgt tggcgaacag ttgcttctcg acaaagattt ccaagtcagc gccatagctg
     4441 gtccggactt cctctgcata gacccgaccg aaagcatcac cctgtttgcg ataggtggcg
     4501 ccgaaggctg cgccccaggt cttgatgtcg tgggtaaagc cgacgttcag gacatagtcc
     4561 gactggtcgt tgaagcggcg ttcgcccagc acgtcgtcga ccttgctgtc cagccacgag
     4621 gcgttgagga agacgccggt ctgttccatg ccgatgaagt ccagcggcgt ggagaggtcg
     4681 aactcgaccc catagacttg gccgtcaccg acgttgtcga tcgactcgat gaaggtgccg
     4741 gctccaccgc tgccttgttc acccgtattg atgtcctcga tcaggtcggt gacgtcgcga
     4801 tagaaggcgt tgatgccgac cacgccgcga cgccccagac gacgctcgaa gccgacgtcc
     4861 aggccccaag ccgtttcagg ctcaaggaag ggattgccgc ggaagtcgtt gtcaccatgc
     4921 tcttcttcga gcttgaccgg actcaggtta tcgaagtttg gacggcgaac cgtgcgcgcc
     4981 accgccaccg tgatccggtc agtgtccgac agggcgaggc gcaggctggc cgacggcagc
     5041 aggatgccat aatcggagcc cgttgtgcgc agatcgtcgg ccactgtctc gtcggtaatc
     5101 tcgacgtcgg tcgtctcata acgaagccca gcctgccatt tcagctggcc ggtttcccca
     5161 gacagcatga cgtaggggtc aatgcgggtt tcctcgagtg tgttcagacc gccgtcgacg
     5221 atctcgaaat cctcaaactc ggcggcggtc aggatgcgga acgcggtcac atcctcggct
     5281 tcgccttcgg cttccagaat ggtcgtatca cgcg
//
LOCUS       DASVTM010000500        11891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_846679,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000500 DASVTM010000000
VERSION     DASVTM010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11891
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846679"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VF633_13770"
     CDS             complement(<1..944)
                     /locus_tag="VF633_13770"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX8472174.1"
                     /translation="MPTLHPVVAEVTARIVERSRATRADYIRRMDAARDSGAGRAKLS
                     CANWAHAFAGQTIADKLTAMDGSKPNVGIVTAYNDMLSAHQPFERFPDVIRKAAREVG
                     GTAQVAGGTPAMCDGVTQGRPGMELSLFSRDAIAMATGVALTHDAFDSAIMLGVCDKI
                     VPGLFMGALAFGHLPVIFAPAGPMPSGIPNAEKARVRALYAQNQVDRATLLESEIGSY
                     HSPGTCTFYGTANSNQMMMELGGLHMPSTAFVHPDTGLRDALTAAAAKRAVELARSGE
                     CRMADVVSEKSIVNMIVALLATGGSTNHAIHLVAMAKAA"
     gene            complement(944..1657)
                     /gene="pgl"
                     /locus_tag="VF633_13775"
     CDS             complement(944..1657)
                     /gene="pgl"
                     /locus_tag="VF633_13775"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX8472175.1"
                     /translation="MLEAFSTLDSWATTIAGRIEETLGAAVIDNDRAAFAGPGGTTPS
                     PIYARLAQADIGWSKIAVTLVDERYVPETSPDSNARLIRDVLLQDKAAAATFIPLYTP
                     EVTVDRAAAVAAHALFDATGGAEGRFDVVLLGMGEDGHICSMFPNSPTLKTLLSPTIK
                     PTVLGVPHGRDGSAPTLERLSINLPWLMKAERVILGLKGAAKREVFEMEAEGDPAIRP
                     IAAMLANHVPLEVVWTEAN"
     gene            complement(1650..3107)
                     /gene="zwf"
                     /locus_tag="VF633_13780"
     CDS             complement(1650..3107)
                     /gene="zwf"
                     /locus_tag="VF633_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX8472176.1"
                     /translation="MTALVLFGGGGDLAMRMLLPSLYFLDHDGLLPDDLKIIGAARSE
                     ESAAEYIAKVRETCESKARADNVWDEAVWTRMEARLDYLAVDATSAESLKPLKDKVGD
                     GSVTSFLAVSPSLYARIVTAMRAAGLAEVDDRVVLEKPVGRDLKTFLEIDDAVADAFS
                     ESQVFRIDHYLGKETVQNLIALRFGNTVFEPLWNAQNIDHVQITVGETVGVGDRWPYY
                     DEYGALRDMLQNHMLQLLCLVAMEPPSDLDPESVRNEKVKVLRSLRPITDEDAERVTV
                     RGQYVGGAVDGETVKGYDEERGQASDTETFVAIRADIDNWRWAGVPFFMRTGKRLPEK
                     RTEIVIQFKPLPHSIFHYEDGEPQANRMIIELQPEEDISLTVMNKKPGLDSRMQLQPI
                     KMSLSWGTRSKTDAPLRRRIAYERLLLDALKGDSTLFVRRDEAEQAWKWVDEVSQAWV
                     DGHVKPQEYVAGTWGPDDADMLMARTGRDWNTTDA"
     gene            complement(3136..3771)
                     /gene="hisIE"
                     /locus_tag="VF633_13785"
     CDS             complement(3136..3771)
                     /gene="hisIE"
                     /locus_tag="VF633_13785"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEX8472177.1"
                     /translation="MINPDTLDFAKGDGLIPAIVQDADTLQVLTLAYMDRAALDETIA
                     SGEATFFSRSRGGRWRKGETSGNRQTVMSITQDCDGDALVLAVRPMGPACHLGTTSCF
                     GDSDAPGLGRIAALERTIAERASQDPSVSYTARMIAEGPKRLAQKVGEEGVETALAGV
                     AGPDTELASEAADLVYHLLVLLRARHMVFQDVLDVLAQRAQPAKDASQDGA"
     gene            complement(3768..4535)
                     /gene="hisF"
                     /locus_tag="VF633_13790"
     CDS             complement(3768..4535)
                     /gene="hisF"
                     /locus_tag="VF633_13790"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX8472178.1"
                     /translation="MTARRIIPCLDVKDGRVVKGVRFEGHVDMGDAAELAARYRDEGA
                     DELVFYDITASPEGRTLDYAWVKSIARLLDIPFCVAGGIRSVDQAAACLDHGADKVSI
                     NSPALERPELIAEMAERLGSQCVVVGVDSRSENGEYVVHKFTGDPDSRRLAGKRTLDW
                     LTEAQSLGAGEIVLNCIDQDGVRKGYDVAQLTEARAVLTIPLIASGGAGAVSHFLDVF
                     EKADVSGALAASVFHTGAIAIPDLKASLAKSGLEMRL"
     gene            complement(4532..5266)
                     /locus_tag="VF633_13795"
     CDS             complement(4532..5266)
                     /locus_tag="VF633_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEX8472179.1"
                     /translation="MIVYPAIDLRGGVCVRLMHGRFDQVTEYDQNPARQLATFVGEGA
                     TWVHIVDLDGAQAGRAVQHTLIGELASSFDVAIQSGGGVRSIEDVRNLIDAGVSRVVV
                     GSLAVTKPDEVGEWLGMFGPEQLTLAFDVRVGEHGVPTPSLKGWTEAAGFDLWTALDR
                     YPPGTLTHVLITDVGRDGALTGPNLDLLGEARRRRPDLKIQASGGVSSLDDLTAAAAI
                     GCDGAIVGRAIYEGRFTAAEAIAATA"
     gene            complement(5263..5862)
                     /gene="hisH"
                     /locus_tag="VF633_13800"
     CDS             complement(5263..5862)
                     /gene="hisH"
                     /locus_tag="VF633_13800"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX8472180.1"
                     /translation="MSTVIVGYGAGNTASVEFALERLGCEAVITADPGVIANADRVIL
                     PGVGAAGYAMERLAALGLVEPLRAFQRPLLGVCLGQQLLFERSEEGGGVDLLGFIPGV
                     VTRFDIDPALPVPHMGWSRLEAVKADPLIEGVADGSYAYFVHSFVCPDGGTTLARADY
                     GTVVPAVVRNGNRWGCQFHPERSAEAGARILKNFLELPA"
     gene            complement(5859..7286)
                     /gene="hisB"
                     /locus_tag="VF633_13805"
     CDS             complement(5859..7286)
                     /gene="hisB"
                     /locus_tag="VF633_13805"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX8472181.1"
                     /translation="MTLPAPYAPLVSGGEGLDRYPTEPSALAARMAQIYGVPVEQVLP
                     VRGLTHGLELVWRLAARDGRSVQAPDAQPYSGLAAIYPARGETAAVVIRALGSPEAVA
                     EMAERVAPALLVVDEGLIEFADTASSAATIVGEHANVVVLRSLSLAYGLAGARVGAAI
                     AQPQTLARLTSVLEPYALPEPLVRLAQQALDPSRMIETAERIASVKLERARVAREVAR
                     LMTVESGVGPILMARPNDPEVTLVELAKYGVAADRAGDRLRLPISIKPEVNDRLLLAL
                     GLTPARSRPHRIGQAVRDTKETRIVCAVDLDATGPITIHTGVGFFDHMLEQVAAHGGF
                     SMRLSCEGDLHTDPHHTIEDSAIALGQALKQALGERRGIARYGFVLAMDEAEAHVAID
                     LSGRPYPLFEGTFATPFIGEYRTDLTAHVFRSLAEAMGAAVHIKVTGDDDHHKTEAVY
                     KGFGRALRQAIRVEGDAVPSTKGVL"
     gene            complement(7283..8605)
                     /gene="hisD"
                     /locus_tag="VF633_13810"
     CDS             complement(7283..8605)
                     /gene="hisD"
                     /locus_tag="VF633_13810"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX8472182.1"
                     /translation="MAAVSFKRIDWASLDAAGRKAALARPTQRTEGRVTDVVREIFDD
                     VQARGGKAVTDWSVRLDKAPPRRIAITPAVVSEARNSLAPADTRSIRIAAENVRVFHE
                     MTRPADTAWIETTKGVKSRLVWRSIGAAGLYVPGGSAPLFSSLLMLAIPAGVAGVRER
                     VAVTPPSKDGGVHPAMILAAAEAGLDALWLLGGAQAIAALTFGAELEDGVIPACDKLF
                     GPGNASVAEAKRYASSLPGGPSQDMPAGPSELLVIADGSADPEIAAADLLSQAEHDAD
                     AQVILVSDSTATINAILEQVEEQLDTLPRADIARASLAEARIIKVRNMEAACEVANLY
                     GPEHLALQTEDADDLVERISSAGAVFVGRFAAETLGDYAAGPSHVLPTDGGARTLGGI
                     TTSSFMTTMSVQTVTEAGAAVLAPVAARLARLEGLEAHARAADLRASA"
     gene            complement(8605..9486)
                     /gene="hisG"
                     /locus_tag="VF633_13815"
     CDS             complement(8605..9486)
                     /gene="hisG"
                     /locus_tag="VF633_13815"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX8472183.1"
                     /translation="MSTVQGRLRIAVQKSGRLADRSLDLVRDAGLRVVKGNNDLLYKV
                     ENYPIDLLRVRDDDIPTFVADGVCDLGIVGENVLEEDKNGGANAEIVMPLGFGRCTLK
                     LAVPPTLDYTGPHSLQGQRIATSYPKILRRFLNDQGVQADIVVMRGAVEVAPRLKLSA
                     AICDLVSTGATLEANGLVAKETVLDSQAVLIKSPTAPEPGLQHLLDSIIERMAGVVSS
                     QGAKYVMLNAPRSALDQITAILPGAGSPTVMPLMGRDDAVAVHAVCQEAVFWETLEKL
                     KAAGASAILVLPIEKMM"
     gene            complement(9494..9817)
                     /locus_tag="VF633_13820"
     CDS             complement(9494..9817)
                     /locus_tag="VF633_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEX8472184.1"
                     /translation="MLTPISRKAVNQAADDRIALYDALLSLKTREEFDAFLSDLCTPS
                     ELRAFAERWAVARLLFEQGKSYREIAAEAGASPTTVVRVARFLKDMPHQGYSLVLERL
                     KKKKA"
     gene            10049..11452
                     /locus_tag="VF633_13825"
     CDS             10049..11452
                     /locus_tag="VF633_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX8472185.1"
                     /translation="MSGASGSGEVRPSYRTLARWLENAPSDLLQSRSRQAELFFRRMG
                     VTFAVYGDAESNERLIPFDVVPRIIGAAEWEDLERGLKQRVSAINAFLKDIYGPQECI
                     KAGIVPADLIFTNPHYRPEMQGRRPPGDIWTHICGVDLVRTGEDGFLVLEDNCRTPSG
                     VSYMLENREMMMRLFPDLFAEHRIRPVETYTDMLLSSLQASAPAGAGADPNIVVLTPG
                     PFNSAYYEHSFLADKLGVELVEGGDLLVNDDIVYMRTTEGLKRVDVIYRRVDDEFLDP
                     LTFRPDSQVGIPGIMAAYAAGNVTLSNAVGTGVADDKAVYTYMPDIIRFFSGGEPILG
                     NVPTWRCREPDALKEVLSKLDQLVVKEVGGSGGYGMLVGPASTKAEIEDFRAKLIANP
                     DDFIAQPTLALSTAPTLDGGSLSPRHVDLRPFVLSSPAGVRVAPGGLTRVALKAGSLV
                     VNSSQGGGTKDTWVLDD"
     gene            11452..>11891
                     /locus_tag="VF633_13830"
     CDS             11452..>11891
                     /locus_tag="VF633_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX8472186.1"
                     /translation="MLSRTADSLYWTGRYMERADFLARILEAAVRLAALPAKDGAAAT
                     AWASAIASAGVSKAFAASGRTPSEKSVREYLAFAPDNPSSIRACITAARTNARSVRTA
                     LTIELWEAINGAWNGMSEMGEPAKRDDFVRFLEWVKTISLNVEGA"
BASE COUNT         1982 a         3836 c         4048 g         2025 t
ORIGIN      
        1 gcggctttgg ccatggcgac cagatggatc gcgtggttgg tcgagccgcc ggtggccaga
       61 agagccacga tcatattgac gatcgacttc tccgacacga cgtctgccat gcggcactcg
      121 ccgctgcggg ccagctcgac agcgcgcttt gcggctgcgg ctgtcagggc gtcgcgcaga
      181 ccggtatccg gatggacaaa ggcggtcgaa ggcatatgca ggccgccgag ctccatcatc
      241 atctggttgg agttggcggt gccgtagaag gtgcaggtgc cgggcgaatg gtaggagccg
      301 atctcgcttt ccagcagggt cgcgcgatcg acctggttct gggcgtagag ggcgcggacg
      361 cgagctttct cggcgttcgg aataccggat ggcatgggac cggccggggc gaagatcacc
      421 ggcagatggc cgaaggccag agcgcccatg aacagtccgg gaacgatctt gtcgcacacg
      481 cccagcataa tggccgagtc gaaggcatcg tgggtcaggg ccacgcctgt cgccatggcg
      541 atggcgtcgc ggctgaacag cgacagctcc atgcccgggc gtccttgggt gacgccgtcg
      601 cacatggcgg gcgtgccgcc ggcgacctga gcggtgccgc cgacttcgcg cgccgctttg
      661 cggatcacat cggggaagcg ctcgaacggc tggtgggccg agagcatgtc gttataggcg
      721 gtgacgatgc cgacgttggg tttggacccg tccatcgccg tcagcttgtc tgcgatggtc
      781 tggccggcga aggcgtgggc ccagttggcg caactgagct tggcgcgacc ggcgcccgag
      841 tcacgagcgg cgtccatgcg acggatgtaa tcggcgcggg tagccctgga gcgctcgacg
      901 atgcgggcgg tgacttccgc gactacagga tgaagtgtcg gcatcagttg gcctccgtcc
      961 acacaacttc gagcggaacg tgattggcga gcatggcggc gataggacgg atggcgggat
     1021 cgccctcggc ctccatctcg aacacctcac gtttagcggc gcccttgaga ccgaggatga
     1081 cccgctcggc cttcatcagc cagggcaggt tgatcgacag acgctccagc gtaggggccg
     1141 agccgtcgcg gccgtgtggc acgccaagaa cggtcggctt gatggtcggg ctgagcaggg
     1201 tcttcagcgt cgggctgttg gggaacatcg aacagatatg gccgtcctcg cccatcccaa
     1261 gaaggacgac atcgaagcga ccctcggctc cgcccgtggc gtcgaacagg gcgtgggcag
     1321 cgacggcggc ggcgcgatca accgtcacct cgggcgtata gagcgggatg aaggtcgcgg
     1381 ccgcagcctt gtcctgcagc aagacgtccc ggatcaggcg cgcgttcgag tccggcgagg
     1441 tctcgggcac atagcgctca tccaccaagg tcacggcgat cttcgaccag ccgatgtcgg
     1501 cttgggccag tcgggcatag atcggcgagg gcgtcgttcc tccggggccg gcgaaggccg
     1561 cacggtcgtt gtcgatgacc gcggccccga gcgtttcctc gatccggccg gcgatggtcg
     1621 tcgcccagct gtcgagtgtc gaaaaggcct caagcatcgg tggtgttcca gtcgcggccg
     1681 gtacgggcca tcagcatatc cgcgtcgtcc ggaccccagg tcccggcgac atattcctga
     1741 ggcttaacat ggccatcgac ccaggcttgt gaaacctcgt cgacccattt ccacgcctgc
     1801 tcggcttcgt cgcggcgcac gaacaaggtc gagtcgcctt tcagggcgtc gagcaacagc
     1861 ctctcatagg cgatgcggcg gcggagcggg gcgtcggtct tgctgcgagt tccccagctc
     1921 aggctcatct tgatcggctg caactgcatc cggctgtcga ggcccggctt cttgttcatc
     1981 accgtcagcg agatgtcttc ctcgggctgc agctcgatga tcatccggtt ggcctgcggt
     2041 tcgccgtcct cgtagtggaa gatggagtgg ggcaggggct tgaactggat cacgatctcg
     2101 gtgcgcttct cgggcagtcg cttgccggtg cgcatgaaga acggcacccc ggcccagcgc
     2161 cagttgtcga tgtcggcgcg aatggcgacg aaggtctccg tgtcagaggc ctgaccccgt
     2221 tcctcgtcgt agccctttac cgtttcacca tcgacggcgc cgccgacgta ctggccgcgc
     2281 acggttaccc gttcggcgtc ctcgtcggtg atcggacgta acgaccgcaa cacctttacc
     2341 ttctcgttgc gcacggactc ggggtcgagg tccgaaggcg gctccatggc cacgaggcac
     2401 agcagctgca gcatgtggtt ctgcagcatg tcgcgaagcg cgccatactc gtcataatag
     2461 ggccagcgat cgccgacgcc gacggtctcg ccgacggtga tctggacgtg gtcgatattc
     2521 tgggcgttcc acagcggctc gaacacggtg ttgccgaagc gcagggcgat caggttctgg
     2581 accgtctcct tgccgagata gtgatcgatg cggaagacct gcgattccga gaaggcgtcg
     2641 gcgacagcgt cgtcgatctc aaggaaggtc ttcaggtcgc gaccgaccgg cttttccagc
     2701 acgacccggt catcgacctc ggccagaccg gcggcgcgca tggccgtgac gatcctggca
     2761 tacagcgacg gcgaaacggc gaggaaggat gtgaccgatc cgtcgccgac cttgtccttg
     2821 aggggcttca ggctttcggc gctggtggcg tcgacggcca gatagtcgag ccgggcctcc
     2881 atccgcgtcc agacggcctc gtcccagacg ttgtcggccc tagccttgga ctcgcaggtc
     2941 tcgcggacct tggcgatata ctcggctgcg ctctcctccg agcgggcggc gccgatgatc
     3001 ttcagatcgt caggcagcag tccgtcatgg tcgagaaaat agagcgaggg gagcagcatc
     3061 cgcatggcga gatcgccacc gccgccgaac agcaccaaag ccgtcatgcc ggttccttcc
     3121 ttaggacggg agccgtcaag ctccgtcttg cgatgcgtct ttggccggtt gggctcgctg
     3181 agccaacacg tccaacacgt cctgaaacac catgtgacgc gcccgtaaca gcaccagcag
     3241 atgatagacc aaatctgcgg cctcgctggc aagttccgta tccggtccgg cgacacctgc
     3301 cagcgcggtt tcgacaccct cctcgccgac cttctgggcc agacgctttg gaccttcggc
     3361 gatcatgcgg gcggtatagc tgaccgacgg atcctgcgag gcgcgctcgg cgatagtgcg
     3421 ttccagcgct gcgatccggc cgagacccgg cgcgtcgctg tcgccgaagc aactggtggt
     3481 gccgaggtgg caggccggcc ccatcgggcg cacggcgagg accagagcat ccccgtcgca
     3541 gtcctgcgta atcgacatga cggtctggcg gttgcccgag gtctcgccct tgcgccaccg
     3601 tccgccgcgc gagcgcgaga agaaggtcgc ctcgccggat gcgatggtct cgtccagcgc
     3661 ggcgcggtcc atataggcca gcgtcagcac ctgcagcgtg tcggcgtcct gaacgatggc
     3721 cgggatcagg ccatcgcctt ttgcgaaatc gagagtatcg gggttgatca cagtctcatc
     3781 tccaggccgg atttcgccag cgaggccttc aggtcgggaa tggcgatagc gccggtgtgg
     3841 aagacgctgg cggcgagggc gccggacacg tccgcctttt cgaacacgtc gaggaaatgc
     3901 gacacggcgc ccgctccacc ggaggcgatc aggggaatgg tcaacaccgc gcgagcctcg
     3961 gtcagttggg ccacgtcata gcctttacgc accccgtcct gatcgatgca gttgagcacg
     4021 atctcaccgg cgccgaggct ttgcgcttcc gtcaaccagt ccagcgtccg cttgcctgcc
     4081 aggcgacggc tgtccggatc gccggtgaat ttgtgtacga cgtattcgcc gttctcgctg
     4141 cggctgtcga cgccgacgac gacgcactgg ctgcccagtc gttcggccat ctcggcgatc
     4201 agttcgggac gttcgagtgc gggtgagttg atcgagacct tgtcggcgcc gtggtcgaga
     4261 caggcggcgg cctgatcgac gctgcgaatg ccgcctgcaa cgcagaacgg gatgtcgagc
     4321 agacgcgcga tcgacttgac ccaggcatag tccaacgtcc ggccttcggg gctggcggtg
     4381 atgtcgtaga agaccaattc gtcggcgccc tcgtcacgat agcgggcggc aagttcggcg
     4441 gcgtcaccca tgtcgacatg accctcgaag cgaacgccct tcacgacccg gccgtccttg
     4501 acgtcgagac acgggatgat gcgccgagcc gtcatgcggt cgccgcgatg gcttcggcag
     4561 ccgtgaagcg gccttcgtag atcgcgcgcc cgacgatggc cccgtcgcaa ccgatagcgg
     4621 cggcggcggt cagatcatcc agcgaagaga cgccgcccga ggcctgaatc ttcagatcgg
     4681 gacgacggcg acgcgcctcg cccagcaggt cgagattagg tccagtcagg gctccgtcgc
     4741 ggccgacgtc cgtgattagc acgtgagtca gggtgccggg aggatatcgg tccagcgcgg
     4801 tccacaggtc gaaaccggca gcctcggtcc agcccttgag cgatggcgtc ggaacgccgt
     4861 gctcacccac gcgcacgtcg aaggcgagcg tcagttgttc gggcccgaac atgccgagcc
     4921 attcgccgac ctcatccggt ttggtcacgg ccagcgatcc gaccacgacg cgcgacacgc
     4981 cggcgtcgat caggttgcgg acgtcctcga tggaccgcac cccaccgccc gactggatcg
     5041 ccacgtcgaa gctggaggcc agttctccga tcagggtgtg ctgcaccgcg cgcccggcct
     5101 gtgcgccgtc gagatccacg atatggaccc aggtagcgcc ctcgccgacg aaggtcgcca
     5161 gttggcgcgc ggggttctgg tcgtattcgg tgacctgatc gaagcggcca tgcatcagcc
     5221 gcacgcagac gccgccgcgc aggtcgatgg cgggatagac gatcatgcgg gcaactcgag
     5281 gaagtttttc aggatacggg cgccggcctc ggccgagcgc tcgggatgga actggcagcc
     5341 ccaacggttg ccgttgcgaa ccacggccgg gacgactgtg ccgtagtctg cgcgcgcgag
     5401 cgtcgtccca ccgtccgggc agacgaagct gtgcacgaaa taagcatatg acccgtcagc
     5461 gacgccttcg atcagcggat cggccttgac ggcttcaagc cggctccagc ccatgtgcgg
     5521 gacgggcagt gcggggtcga tatcgaacct ggtcaccacg ccgggaatga agccgagcag
     5581 atcgacgccg ccgccttctt cactacgttc gaatagcagt tgttgcccga ggcatacccc
     5641 cagcagcggg cgctggaagg cgcgcaacgg ctcgaccaga cccagcgccg ccagccgctc
     5701 catggcataa cccgcggctc ccacgcccgg caggatgacc cggtcggcgt tcgcgatcac
     5761 gcccggatcg gcggtgatga cggcctcgca ccctagccgt tccagcgcga actcgaccga
     5821 cgcggtattg cctgcgccgt agccgacgat gacggtgctc acagcacccc cttggtgctc
     5881 ggcactgcgt caccctcgac gcgtatggcc tgacgcaggg cgcgaccgaa gcctttgtag
     5941 acggcctcgg tcttgtggtg atcgtcgtcg ccggtgacct tgatgtgcac cgccgcgccc
     6001 atggcctcgg ccagcgagcg gaacacgtgg gccgtcagat cggtgcggta ttcgccgatg
     6061 aatggcgtcg cgaacgtgcc ctcgaacagc ggatagggcc gccccgacag gtcgatggcg
     6121 acgtgggcct ctgcctcgtc catggccaga acgaagccat agcgagcgat gccgcgacgc
     6181 tcgccgagcg cctgcttcag cgcctgaccc agagcgatgg cgctatcctc gatggtgtga
     6241 tgcggatcgg tgtgcaggtc gccttcgcac gacaggcgca tcgagaagcc gccatgggcc
     6301 gcgacctgct ccagcatatg gtcgaagaag ccgacgccgg tgtggatcgt gatcggccca
     6361 gtcgcgtcga gatcgacggc gcagacgatg cgcgtttcct tggtgtcgcg gacggcctga
     6421 ccgatcctgt gcggacggct tcgagcgggg gtgagaccca gcgccagcag caaacggtcg
     6481 ttgacctccg gcttgatcga gatcggcagg cgcagacggt cgccggcccg gtccgccgcc
     6541 acgccgtatt tggccagctc caccagcgtc acctcgggat cgttcgggcg cgccatcagg
     6601 atagggccga cgccgctctc gaccgtcatc agtcgtgcga cttcgcgggc gacgcgggcg
     6661 cgctccagtt tgaccgaggc gatgcgctcg gcggtctcga tcatccgcga agggtccagc
     6721 gcctgctgag ccagccgcac cagcggttcc ggcaaggcgt agggctccag cacagacgtc
     6781 aatcgcgcca gcgtttgcgg ctgggcgatg gccgcgccga ctcgcgctcc ggccagaccg
     6841 taggccagcg acaggctgcg caggacgacg acgttggcgt gctcgccgac gatggtggcg
     6901 gcggaagaag cggtgtcggc gaattcgatc aggccttcgt cgaccaccag cagggccggg
     6961 gcgacgcgtt cggccatctc ggcgaccgct tcgggcgagc cgagcgcgcg gatgacgacg
     7021 gcggcggtct cacctcgtgc cggatagatg gcggcgagac cggagtaggg ctgggcgtcg
     7081 ggcgcctgca ccgaccgacc gtcacgagcg gccagacgcc agaccagctc cagcccgtgg
     7141 gtcaggccgc gcaccggcag gacctgttcg acgggcacgc catagatctg ggccatgcgc
     7201 gcggcgagcg ccgacggttc ggtcggatag cggtcgaggc cttcgccgcc ggaaaccagc
     7261 ggagcatagg gagcgggcag ggtcatgcgg acgccctcag atctgccgcc ctcgcatgag
     7321 cttccagacc ttccagtcgg gccagacggg cggcgaccgg agcgagaacc gcggcacccg
     7381 cctcggtcac cgtctgcacc gacatcgtgg tcatgaagct ggaggtggtg atgccgccca
     7441 gcgtccgcgc gccaccgtct gtcggcagga cgtgcgacgg cccggcggcg tagtcgccta
     7501 gcgtttcggc ggcgaaccga ccgacgaaga cggctccggc ggagctgatc cgttcgacca
     7561 gatcgtcagc gtcctcggtc tgcaatgcga ggtgttcggg gccgtagagg ttcgccacct
     7621 cgcaagccgc ttccatgttg cgcaccttga tgatccgggc ttcggcaagt gacgcgcggg
     7681 cgatgtcggc gcggggcagg gtgtcgagtt gttcctcgac ctgttcgagg atggcgttga
     7741 tcgtggcggt cgagtcgctg accaggatga cctgcgcgtc ggcgtcgtgc tcggcctggc
     7801 tgagcaggtc ggcggcggcg atctccgggt cggccgagcc gtcggcgata accagcaact
     7861 cggacgggcc tgcgggcatg tcttgagacg gcccgccggg cagtgacgag gcatagcgct
     7921 tggcctcggc gacagaggcg ttgccgggcc cgaacagctt gtcgcatgcc gggatcacgc
     7981 cgtcttccag ttcagcgccg aacgtcagcg cggcgatggc ctgggcgccg ccgagcagcc
     8041 acagcgcatc gagcccggcc tctgcggcgg cgaggatcat ggcggggtga actccgccgt
     8101 cttttgaggg tggcgtgacg gcgacccgct cgcgcacgcc cgcaacgccc gccggaatgg
     8161 ccagcatcaa cagcgacgag aacaagggtg cggaaccgcc gggcacgtag agtcctgccg
     8221 cgccgatcga gcgccagacg aggcgggatt tgacgccctt ggtcgtctcg atccaggccg
     8281 tgtcggcagg cctggtcatc tcgtggaaga cgcggacgtt ctcggcggcg atgcggatcg
     8341 agcgggtgtc ggcgggcgcc aaagagttgc gcgcctcgga caccacggcg ggcgtgatgg
     8401 cgatgcggcg cggtggggcc ttgtcgagcc tcacgctcca gtcggtgacg gccttaccgc
     8461 cacgtgcctg aacgtcgtcg aagatctcac gcacgacatc cgtcacgcgg ccttcggtgc
     8521 gctgcgtcgg gcgggccaga gccgcctttc gcccggcggc gtccagggag gcccagtcga
     8581 tgcgtttgaa gctgaccgca gccatcacat catcttctcg atcgggagga ccagaatagc
     8641 cgaggcgccg gcggccttca gcttctccag cgtctcccag aacacggctt cctgacagac
     8701 ggcgtggacg gccaccgcgt catcgcggcc catcagcggc atgacggtcg gcgaaccggc
     8761 gcccggcagg atggcggtga tctggtccag cgccgagcgg ggggcgttga gcatgacgta
     8821 tttggcgcct tgcgaggaca ccacgcccgc catccgctcg atgatggaat ccagcaggtg
     8881 ctgcaggccg ggttccggtg cggtcggcga tttgatcagc acggcctggc tatccagcac
     8941 ggtttccttg gcgaccagac cattggcttc cagcgtcgcg ccggtcgaaa ccagatcgca
     9001 gatggcggcg gaaagcttaa ggcggggggc gacctcgacc gcgccgcgca tgacgacgat
     9061 gtcagcctgc acgccctgat cgttgaggaa gcggcgcagg atcttgggat aggaggtggc
     9121 gatccgctgg ccctgcagcg agtgcggtcc ggtgtaatcc agcgtcggcg gaacggccag
     9181 cttcagcgtg caacgcccga agcccagcgg catgacgatc tcggcgttcg cgccgccgtt
     9241 cttgtcttcc tccagcacgt tttcgccgac gatgccgagg tcgcaaacgc cgtcggcgac
     9301 gaaggtcggg atgtcgtcgt cacgcacgcg cagcaggtcg atcggatagt tctcgacctt
     9361 gtagagcagg tcattgttgc ccttgaccac gcgcaggccc gcatcgcgga ctaggtcgag
     9421 gctgcggtcg gccagacggc cggatttctg cacggcgatg cgaagacgcc cttggacggt
     9481 actcatgacg ggatcaggct ttcttctttt tcagtcgctc tagcaccagg ctgtagccct
     9541 gatgcggcat gtctttgagg aaccgggcga cgcgcaccac ggtggttggg ctggcgcccg
     9601 cttcagcggc gatctcgcgg taggacttgc cctgttcgaa gagcagccgc gccaccgccc
     9661 agcgctcggc gaaggctcgc agttccgatg gtgtgcaaag atccgacagg aaggcgtcga
     9721 attcctcgcg cgtcttcagg gacagcaagg cgtcatagag cgcgatgcgg tcgtctgcgg
     9781 cctgatttac ggccttgcgg gagatcgggg tcagcatggt gcgttccact agagtgtaac
     9841 agtggaacgg tcaagggatg gtttttacgg tgtatagggg tgatcgggat tggcggtgaa
     9901 gcttgccgcg atacagcgca gtcgggtagc gcggcgacac gagttcgact gaaatcgtct
     9961 tcgcgttgca acatttctgt tgcgcgagag gacggccccg gctcaatctg gaccttcgca
    10021 aagaaggagc agggccacgt tcgacgagat gtcaggcgca agcggatccg gtgaggtccg
    10081 cccaagttac cggacgctgg cccggtggct ggaaaacgcg ccgtccgatc ttctgcagtc
    10141 tcgttcgcga caagccgaac tgttcttccg ccgcatggga gtgactttcg ccgtctatgg
    10201 cgatgccgaa tccaacgagc ggctgatccc cttcgacgtc gtgccgcgca tcatcggcgc
    10261 cgccgaatgg gaggatctcg agaggggcct caagcagcgc gtctctgcga tcaacgcctt
    10321 cctgaaggat atctacggcc cgcaggaatg catcaaggcg ggtatcgtcc cggccgacct
    10381 gatcttcacc aatccccact atcgcccgga aatgcagggc cggcgcccgc cgggcgacat
    10441 ctggacccat atctgcggcg tcgatctggt ccgcacgggc gaggatggct tcctcgtgct
    10501 ggaagacaac tgccgcaccc cctcgggcgt ctcctacatg ctggagaacc gcgagatgat
    10561 gatgcggttg ttccccgacc tgttcgcgga gcaccgcatc cggccggtcg aaacctatac
    10621 cgacatgctg ctgtcgagcc ttcaggcttc ggcgccggca ggggcgggtg cggatccgaa
    10681 catcgtggtc ctgacgccgg gaccgttcaa ctccgcctat tacgaacact cgttcctggc
    10741 cgacaagctg ggtgtcgagt tggtcgaggg cggcgatctg ctggtcaacg acgacatcgt
    10801 ctacatgcgc acgaccgagg gtctgaagcg cgtcgacgtc atctatcgcc gtgtcgacga
    10861 cgagttcctc gacccgctca ccttccgtcc ggattcgcag gtcggcatcc cgggcatcat
    10921 ggcggcctat gcggcgggca acgtcaccct gtcgaacgcg gtcggcacgg gcgtggcgga
    10981 cgacaaggcc gtctatacct atatgcccga catcatccgc ttcttctcgg gcggggagcc
    11041 gatcctcggc aacgtgccga cctggcgttg tcgcgagccg gacgcgctca aggaagtgct
    11101 gtccaagctg gaccagttgg tcgtcaagga agtcggcggt tccggcggtt acggcatgct
    11161 ggttggcccg gcttcgacaa aggccgagat cgaggatttc cgggccaagc tgatcgccaa
    11221 cccggatgac tttatcgccc aaccgacgct ggcgctgtcg acagcgccga cgctggacgg
    11281 cggttcattg tcgccccgtc acgtcgacct gcgtccattc gtgttgtcga gcccggccgg
    11341 ggtgcgcgtt gcgcccggcg gcctgacacg ggtcgcgctc aaggccggat ccctggtggt
    11401 caactccagt cagggcggtg gaaccaagga cacctgggtg ctggacgact gatgctttcc
    11461 cgtactgcag acagcctcta ctggaccggc cgctatatgg agcgggcgga tttcctcgcc
    11521 cggatcctcg aagccgccgt gcgcctcgcc gccctgccgg ccaaggacgg cgcggccgcg
    11581 accgcatggg ccagcgccat cgcctcggca ggggtatcga aggcgttcgc cgcctcaggc
    11641 cgtaccccga gcgagaagtc ggtgcgcgag tacctcgcct tcgccccgga caatccatcg
    11701 tcgatccgcg cttgtatcac cgccgcccgc accaatgcgc gctcggtccg cacggccctg
    11761 acgatcgagc tctgggaagc catcaacggc gcctggaacg gcatgtcgga aatgggcgaa
    11821 cctgccaagc gcgacgactt cgtgcgcttc ctcgagtggg tcaagacgat ctcgctgaac
    11881 gtcgagggcg c
//
LOCUS       DASVTM010000501         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_898014,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000501 DASVTM010000000
VERSION     DASVTM010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898014"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VF633_13835"
     CDS             complement(<1..1037)
                     /locus_tag="VF633_13835"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX8472187.1"
                     /translation="MRACLAALAACVFCVMPARAADYAIFDPVVVSALSSTRATQPAM
                     ATIASADAALVRAPGPLAVVHTEGTLPGQGIRDVSLVARRDLPTMLDLALAYRLTGER
                     KYLDVANRFLLAWADTYVVSLNPIDETNFDAMILAYDLTEPDLPAQTREAMDRFLRNL
                     ATGYLDDMDGAPKHFYTNWQSHRIKIAALASFQLGDPRLIERTFEDYRKHVASNVLAD
                     GTVFDFYERDAIHYVLYNVDPMMVAALAARAHGQDWFGWKNASGTGVSSVIDWLIPYV
                     EGAKTHQEFVHSRISFDAERLAAGQTEYAGPWRPERAVNTLALASLLEDRYPSALNAL
                     LATTNRMPPSWV"
     gene            1368..>2694
                     /locus_tag="VF633_13840"
     CDS             1368..>2694
                     /locus_tag="VF633_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8472188.1"
                     /translation="MSALALAGSIAFASGAAAQTSSNPQSSPAPSSTAQDETDTVDEV
                     VVTGFRGAYASALQTKRSSDQITDSISSDGLGRFPDLNVGEAIQRLPGVQINREADSR
                     NATISLRGLPGSFARTTLNGSGFADPILSTSTNMGSTPLGAFNSDIFSGITIIKSPDA
                     SNVAGGLSGIIDLRIASALSRRDGGFVKAAYEYNELGSQGSPSYTVGYNTHLTDTLAV
                     FGTLAFKEENFRRDSISVNSWANRLGSIQVGNQGVPGSNPVYDALIAQNPGGVYYPSQ
                     MRQFVRQNRGDVLTGAAGFEWQATDELKLGLTGFYTKRQLDEGTNHLLYIENVAGANT
                     NTALNATSAVTRFTSIGTPYVAADGRAYINTFSTENAQTFDSVRSEPVTQKVWALNPT
                     VEFSNDTWRATLEGTISRADAQADQIELDIVQNANRNLGPAGLNGITSSV"
BASE COUNT          533 a          868 c          821 g          472 t
ORIGIN      
        1 acccaagatg ggggcatcct gttcgtcgtc gcgagcagcg cgttcaaagc tgacggataa
       61 cgatcctcaa gaaggctggc gagcgcgaga gtgtttacgg cccgttccgg acgccatggc
      121 ccggcgtatt ctgtctgacc cgccgcgagt ctctcggcat cgaaactgat ccgggaatga
      181 acgaactctt gatgggtttt cgccccttcg acgtagggga tcagccagtc gatgacggac
      241 gacacacccg taccggaggc gtttttccag ccgaaccagt cctggccatg cgcgcgtgca
      301 gcgagggccg cgaccatcat ggggtcgaca ttgtagagca catagtggat cgcatcgcgc
      361 tcgtagaagt cgaacacggt cccgtcggcg aggacgttgc tcgccacatg tttccggtag
      421 tcctcgaacg tccgctcgat cagtcgcggg tcgccgagct ggaaggatgc gagggcggcg
      481 atcttgatgc gatggctctg ccagttggtg tagaaatgtt tgggcgcgcc atccatatcg
      541 tcgaggtagc ccgttgcgag atttcgcaaa aagcgatcca tggcctcgcg ggtttgagcc
      601 ggcagatccg gctccgtcag gtcgtaggcc aggatcatgg cgtcgaaatt cgtctcgtcg
      661 atcggattca aggacacgac gtaggtgtcg gcccaggcga gaagaaaccg gttggcgaca
      721 tcgagatatt ttcgctcgcc ggtcaggcgg taggcgagcg caaggtccag catggtgggc
      781 aagtcacgcc gtgcgacaag gctgacatcg cgaatgccct gaccgggcaa ggtgccttcc
      841 gtatgcacga ccgcgagagg gccaggcgca cgcacgagcg cggcatccgc gctggcgatc
      901 gtcgccattg cgggctgggt ggcgcgggtt gaggagagcg cagatacgac caccgggtcg
      961 aatatcgcat agtccgctgc gcgggctggc atgacgcaga aaacgcaggc ggccagcgcc
     1021 gccagacagg ctctcatgag acacttcctt tctctattgg gccagacgac ggcggatcga
     1081 ggctatttac acgagccagt ccggtcaact ggcctgacca aactacatct gccagcgcgc
     1141 agcctcttcc atcgtcagcc ttgtccatag ccatctagcc gttgctcaaa ccaggtcggc
     1201 gcaattccag aacgggtttg ttgctcaagc gctgaaggta acggcagacc actctttcaa
     1261 agcggcttga ccagttgcga gaacgccgta aggtcgcgct cagccgtcga ttgagaacgg
     1321 cacggggtag gaaaatgatt agagaacgga ctctcctttt gagcgccgtg agcgccctcg
     1381 cgctcgccgg ctcaatcgcc ttcgccagcg gtgcggcggc gcagaccagc tctaatcccc
     1441 aatcatcgcc ggcgccctcg tcaacggcgc aggacgagac cgacacggtc gatgaagtcg
     1501 tcgtcaccgg ctttcgcgga gcctacgcca gcgccctcca gaccaagcgc agttccgatc
     1561 agattacaga ctcgatctca tccgacggtc tgggccgctt tcccgacctg aacgtcggtg
     1621 aggcgatcca gcgactgcca ggcgtgcaga tcaatcgcga ggccgactcg cgcaacgcta
     1681 cgatcagcct gcgtggccta ccgggctcgt tcgcccgcac caccctgaac ggatccggtt
     1741 tcgccgatcc gatcctgagt acctcgacca atatgggctc cacgccgctg ggagccttca
     1801 actccgatat cttctcgggg atcaccatca tcaagtcgcc cgacgcctcc aacgttgccg
     1861 gcgggctttc ggggatcatc gacctgcgga tcgcctcggc cctgtcgcgg cgagacggag
     1921 gcttcgtcaa ggcggcctac gagtacaacg aactggggtc ccaggggtcg ccgtcctaca
     1981 cagtgggcta caatacccat ctgactgaca cactcgccgt cttcggcacc ctggccttca
     2041 aggaagagaa tttccggcgc gactccatct cggtcaacag ctgggcgaac cgtctcgggt
     2101 ccatccaggt cggcaaccag ggcgtaccgg gctcaaaccc ggtctatgac gccctgatcg
     2161 cccagaaccc gggcggcgtc tactatccca gccagatgcg tcagttcgtg cgccagaacc
     2221 ggggcgacgt cctgaccggc gcggcgggat tcgagtggca ggcgaccgat gagctcaagc
     2281 tcggcctgac cggcttctac accaagcgcc agctcgacga ggggaccaac catctcctct
     2341 atatcgagaa cgtggccggg gcgaacacca acaccgccct gaacgccacc tcggcggtga
     2401 cccgtttcac cagcatcggc accccctatg tggcggccga cgggcgggcc tatatcaaca
     2461 cgttctcgac cgaaaacgcc cagacgttcg actcggtgcg atcggagcct gtgacccaga
     2521 aggtctgggc cctgaacccg accgtggagt tctccaacga cacctggcgc gccaccctgg
     2581 agggcacaat ctcccgagcc gacgcccagg ccgaccagat cgaactggac atcgtccaga
     2641 acgccaatcg caatctgggc cctgcgggac tgaacgggat cacgtcgtcg gtct
//
LOCUS       DASVTM010000502         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_48774, whole
            genome shotgun sequence.
ACCESSION   DASVTM010000502 DASVTM010000000
VERSION     DASVTM010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48774"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VF633_13845"
     CDS             complement(<1..673)
                     /locus_tag="VF633_13845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8472189.1"
                     /translation="MMSNVYPSLAGKVVVVTGGGSGIGAALTEGFARQGARVVFLDVA
                     DEDSTALADNLSSLSPAPVYRRCDLMNLEALQATIAAIAAEVGPIEVLVNNAANDDRH
                     SLAEVTAAYWDDRIAVNLRHLYFAAQAVAPAMRAAGQGVILNLGSISWHLGLPDLSLY
                     ETAKAGIEGMTRGLARELGVDGVRVACIVPGNVKTPRQMKWYTPEGEADIVAAQCLKG
                     RIEPRD"
     gene            complement(670..1815)
                     /locus_tag="VF633_13850"
     CDS             complement(670..1815)
                     /locus_tag="VF633_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8472190.1"
                     /translation="MRLINILPDDWYEATLMGRIQTAAGPTPVLLRRGRILDISTFAA
                     TGADALSRADVAAATGEDLGAVESQTAASVWADGDLNLLSPLDLQCVKASGVTFAVSA
                     LERVIEEKARGEASVAAGIRARLSERIGGDLASVKPGSEQAAELKTALIADGLWSQYL
                     EVAIGPDAEIFTKAPCLSSVGWGAHVGVRPGSAWNNPEPEIVIVCDPAGKPIGAALGN
                     DVNLRDIEGRSALLLGKAKDNNASASVGPFIRLFDDGFTMDDVRDATVSLEVNGPEGY
                     VLSGASSMRLISRDPEDLARQASGANHQYPDGFALYLGTMFAPIEDRDTPGGGFTHKI
                     GDRVKISSPRLGVLENEVTTCDRAEPWTMGILALMKNLAGRGLLRSA"
     gene            complement(1922..2923)
                     /locus_tag="VF633_13855"
     CDS             complement(1922..2923)
                     /locus_tag="VF633_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8472191.1"
                     /translation="MQPHDLVTISDVAAQAGVSTMTVSRVMNNRGAVKPETREAVLMA
                     VEALGYAPNAAARSLASGGLDRIGLLYSNPSAGYLSEFLLGALEGVQANGGQLTVEQC
                     DDDAQSEIAAVRRLAAGGIRGLLLPPPHGESIAILAEAERLGLKVVAVAPGAFRSQAI
                     AVRIDDRAAAAEMTRRLLDLGHRRIGFISGSPNQTASAERLAGFCTEIDARPDAEAMI
                     EAGAFTYRSGLDAAARLLDRTDPPTAIFASNDDMALGAIALAHQRRLDVPGALTVVGF
                     DDTPMATAVWPALTTVRQPIARMAAQAVALLVRSGEAPPETVFPHLLVERGTSALRR"
BASE COUNT          520 a          962 c         1025 g          523 t
ORIGIN      
        1 cgtccctcgg ctcgatccgg cccttgaggc actgggccgc gacgatgtcg gcctcgcctt
       61 cgggcgtgta ccatttcatt tgacgcggcg ttttcacgtt gccgggcacg atgcaggcga
      121 cgcggacgcc gtcgactccc agctcccgcg ccagaccgcg ggtcatgccc tctatcccgg
      181 ccttggcggt ctcatagagc gacaggtcgg gcaggccgag gtgccaggag atggaaccca
      241 ggttcaggat cactccctga cccgccgccc gcatggccgg ggccacggcc tgggctgcga
      301 aatagagatg acgcaggttc acagcaatcc ggtcgtccca ataggcagcc gtgacctcgg
      361 ccagtgaatg gcgatcgtcg ttggcggcgt tgttgacaag gacctcgatc ggcccgacct
      421 cggcagcgat ggcggcaata gtggcctgca gggcctccag gttcatcagg tcgcaacgac
      481 gatagacggg ggcgggcgac aagctggaca ggttgtcggc aagcgccgtg gagtcctcat
      541 cggcaacgtc gaggaacacc acgcgggcgc cctgtcgcgc gaaaccttcg gtcagggctg
      601 cgccgatacc ggatccaccg ccggtgacga ccacgacctt gcctgccagc gacggataga
      661 cgttgctcat catgctgatc tcagcaggcc gcgaccagcg aggttcttca tgagggccag
      721 gatgcccatc gtccagggct cggcgcggtc acaggtggtg acctcgttct ccagcacgcc
      781 gagacgggga gaggagatct tcacgcggtc tccgatcttg tgggtaaaac cgccaccggg
      841 cgtgtcgcga tcctcgatgg gcgcgaacat ggtgccgaga taaagtgcga agccgtcggg
      901 gtattgatgg ttcgcgcccg aggcctgtct ggcaagatcc tcgggatccc ggctgatcag
      961 gcgcattgag ctggctcccg acaggacgta gccttcggga ccgttgactt cgagcgatac
     1021 ggtggcgtcg cgaacgtcgt ccatcgtgaa gccgtcgtcg aacaggcgga tgaacgggcc
     1081 gaccgaggcc gaggcgttgt tgtccttggc cttgccaagc aacagggcgg atcgaccctc
     1141 gatgtcgcgc aggttgacgt cgttgccgag ggcggcgccg atgggtttgc ctgccggatc
     1201 gcagacgatg acgatttccg gctctgggtt gttccatgcc gagcccgggc gcacgccgac
     1261 gtgggctccc cagccgacgg aggagaggca gggggccttg gtgaagatct cggcgtcggg
     1321 gccgatggcg acctcgagat actgcgacca caagccgtcc gctatcagcg ctgtcttcag
     1381 ttcggcagcc tgttccgagc cgggtttgac cgaggcaaga tcgccgccga tgcgctcgga
     1441 caggcgtgcg cggatgccgg cggcgacgga ggcctcgcca cgcgcctttt cctcgatgac
     1501 gcgttcgagg gccgagaccg cgaaggtgac gcccgacgcc ttcacgcact gcaggtcgag
     1561 gggggagaga agattgagat cgccatcggc ccagacagaa gcagccgtct ggctctcgac
     1621 ggcgccgagg tcttcgccgg tcgctgcggc cacatcggcg cgggacaggg cgtctgcgcc
     1681 ggtcgcggca aaagtcgaga tgtcgagaat tctccccctg cgcagcagca cgggcgtcgg
     1741 accggcggcg gtctggatac gccccattag cgtcgcctcg taccagtcat cgggtaagat
     1801 attgatcagg cgcatatttc ctccgttccg atctgcccgg ccatgaaggc ggtgcgactg
     1861 aaatcatgac taccgtgtta gcgctaacat caatcggcgt aaccaggcct gtcaagaagt
     1921 ttcagcgccg gagggcggat gtgccgcgtt cgaccaacag gtgggggaag acagtttcgg
     1981 gcggtgcttc gccacttcga accagaaggg ccaccgcttg agcggccatt ctggcgatcg
     2041 gctgccgtac ggtggtcaga gcaggccaga ccgccgtggc catcggcgtg tcgtcgaagc
     2101 cgacgacggt cagggcgcct ggcacgtcca gtcgccgttg atgcgcaagg gcgatcgcgc
     2161 ccagggccat gtcgtcgttg ctggcgaaga tggcggtcgg cggatcagtc cggtccaaca
     2221 gccgtgcggc ggcgtcgagt cctgagcgat aggtgaacgc tcccgcctcg atcattgctt
     2281 ccgcgtccgg acgggcatca atctcggtgc agaaacccgc cagccgttcg gcgctggcgg
     2341 tctggttcgg tgagccagaa atgaagccga tccggcgatg ccccaaatcc aggaggcgac
     2401 gagtcatttc agcggcggcg gcgcggtcgt cgatgcgtac ggcgatggct tgggagcgaa
     2461 aggcgccggg cgccaccgcg accactttca ggcccaaccg ttcggcctcg gccaggatgg
     2521 cgatcgactc gccatgcggc ggcggcagca gcagcccccg gatccccccc gcagccagtc
     2581 gccggactgc ggcgatctca ctttgcgcgt catcgtcaca ttgctcgact gtcaattggc
     2641 cgccgttggc ctgcacccct tccagagcgc cgagcaggaa ctcgctgaga tagccggcgc
     2701 tagggttgga atagagcagg ccgatccggt ccagcccgcc cgacgccaga cttcgcgccg
     2761 cggcattggg tgcatagccc agagcctcca cggccatgag gacagcttcg cgtgtttctg
     2821 gcttcacagc gccgcggttg ttcattaccc gcgacaccgt catggtcgag accccggctt
     2881 gggcggccac gtcgctgatc gtcacgaggt catgcggctg caagggcttg ggtccctcga
     2941 ggatgtgctc ccgcccagct gcctgaaacg gttatgccaa atatattgtc gtcgctgaga
     3001 agccccattc cggcatgcta gcttcaaagc
//
LOCUS       DASVTM010000503        11120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1058998,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000503 DASVTM010000000
VERSION     DASVTM010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058998"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VF633_13860"
     CDS             complement(<1..330)
                     /locus_tag="VF633_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472192.1"
                     /translation="MNRVSTAGNYQSALLNLMAAQNQQFEAQTRLSTQKVATDMEGFG
                     RGAESLTALKASQNRVQGFIDTGTAVADRLESQDLALNQIGDAVIGARANIAGALATE
                     NINTLMLE"
     gene            complement(330..2447)
                     /gene="flgK"
                     /locus_tag="VF633_13865"
     CDS             complement(330..2447)
                     /gene="flgK"
                     /locus_tag="VF633_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX8472193.1"
                     /translation="MSLNSILNIASSGLATAQTQLRIVSDNVANVNTVGYVRKIADQA
                     SLTTEGMGSGVGIARIRLATDRFLQAASFNANAEASRQNVRYELYDQIQSLFGDPGGD
                     DGFFSQIDSVFSAFAVTAENATSSPLRQEALFKTESLFAESSRISTQIQTVREDADGR
                     IKTAVEKANDLLQQIEALNGDIAKATATSGDASGSQTNQATLIGQLSALMDVKVSQRN
                     NGGVEIRTSAGILLAGAGAATLDYRAAGSVNAESKFNDIYVTEPGGQGRSLNAGLLTG
                     EIKGLLELRDVEAPAAAERLGELMAHVADELNRAHNANSSVPAPTTLTGKNVGQSLET
                     ALTGFTGTTTIALTNPTTGAIATSAKIVFAGSTMTINGVAADPSTFLTTLNTQLGGAA
                     TASFTDGKLTLSSAGTNGVVIADDATSPSAKAGRGFSHYFGMNDLITTDRTATYDIGL
                     TPTSLHGFSPGQTISLRFTEPDGTRMRDLTVAVPTGTGTMSELLTAMNNPVTGVGRYG
                     SFVLDPGGAVTFKGNSGPATVVSVIQDTTTQTPSGVSMTELFGIGGIRAARADGFNVR
                     ADIIANPSKLALAQLNLSATPGTTALSSGDGRGALGLADAGKRSVNFQAGGGSAGGAQ
                     SVSRYASDLSGDIGSRASAAKSRAAGATAVQTEANGRQNAYEGVNLDEELVNMTTFQQ
                     SFNASARLIQAVKDMYDTLLGMT"
     gene            2843..4504
                     /locus_tag="VF633_13870"
     CDS             2843..4504
                     /locus_tag="VF633_13870"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX8472194.1"
                     /translation="MSPVTLTSVPVAPTAPSRAAKAADPASADAFGAMVADIMAEGGD
                     EGVSAAKDVPAKTSPETAADQTTGKPADEDAAQIEASQDKASPQVLNAPLLLMVQPPA
                     PTTDGPETAGAIETAPPAGVRPDGALLADAGSTSSAPIDASESFAPPVSASTTPASGA
                     DPAPPPTRSDPSTNARTAAATATTTTTTATATATATAAAAAAATPAAGVAGAAGAVSP
                     QAKGSPVAAAPGYGAPASGPGLALKTDAQAEAGEDSEPLISTPRSSSQTSVNRAPTDH
                     PAGLPSRTVGGADAQTTSAPNAAAQSPEKSNAATMDVSQAAPAPEDGSPDAPSLLASS
                     STGQLSGGQSAPPSSPTTGTSTFAAQVQAQSHSADAVLTATIDTAPTGQSQPAPQTSS
                     ISTMPVVASFSGLSRATLETTVQIAAQITRQLAGRSTRFEMGLTPEGLGKVNVSMDID
                     ADGQLTARLAFDNPLAAADLRARVDELRGQLQDAGFKLADDALTFSHQDASSRDGGAA
                     EQRRDQNQGRAFAAASRMADDADTTSPVPAWVSLSLTPRGVDMKV"
     gene            4509..5267
                     /locus_tag="VF633_13875"
     CDS             4509..5267
                     /locus_tag="VF633_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEX8472195.1"
                     /translation="MVAAVTTPTTAAANTDAAKTGLATNFDTFLTLLTAQLKNQDPLA
                     PMDSSQFTQQLTQMTGVQQQLLTNDLLTSLLAAQQGGGLGDASNYIGKDVTAVWSADK
                     LTDGKAEWSYELGADAAAASLSVLDSAGKVVWTGPATDLKTGTHDFSWNGKTTAGGQL
                     SDGGVYTLKVSATNAAGKAVDSQVLMTGKVTGVELYDNVPYLHVGNTIIPLSTVISLQ
                     QAAAATTPTTSVVDQAVDALSSAASALNPLKLLS"
     gene            5280..6764
                     /locus_tag="VF633_13880"
     CDS             5280..6764
                     /locus_tag="VF633_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX8472196.1"
                     /translation="MSINSALLAGVSGLTANAAALAAISQNIANVNTVGYKRTAAEFS
                     TVVNSQTNGTGFSAGGVQATARNYVSQGGQLLRTASSTDLGIDGSGFFVVTEQAENLT
                     PTDTRMFTRAGAFTPDKNGFLRNTAGLYLQGWPIDSNGNINTDPSDLNRLRSINIGAV
                     GGTAEPTTRIQINANLDDSQRDNISIAAKDAALAVPGPLAYNTGAANMAQYDETAASP
                     IGTKPDFRMTVPVSDSKGGQRTVAISFLKSATANEWYAEVHAVPATSVETGAGLSNGQ
                     LATGRVLFTQDGRLDIATMKAANLASAGSGPYLFDDFETASITLGASDSGTPAAGMAK
                     WDAASGVGAQTISFDLNTSAGGLSQLNTASGVLSTVTNGTAFGNLSAIKIDKEGFVTA
                     SFDNGVTRRIAQLAIGTFTSSDNLLAVSGNAYQVSQGSGTFNLKVAGAGGAGEIASSQ
                     LEASTVDLSTEFTGLITTQRAYSASSKIITTADEMLAELISIKR"
     gene            6935..7219
                     /locus_tag="VF633_13885"
     CDS             6935..7219
                     /locus_tag="VF633_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX8472197.1"
                     /translation="MLQERRLNNKGEQYVVGPTGTPLTLRDLPPPNTDRWVIRRKAEV
                     VAAVRGGLLSLEDALSQYRLTAEEFIAWQKAIDKWGMQGLRTTRIQSYRS"
     gene            7343..8575
                     /gene="mnmA"
                     /locus_tag="VF633_13890"
     CDS             7343..8575
                     /gene="mnmA"
                     /locus_tag="VF633_13890"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX8472198.1"
                     /translation="MPFMTAVASLPADDFCVLPEIATADMDQAVEAARLAVGLPVGSR
                     IVAAMSGGVDSTVVAALLHKAGYDVVGVTLQLYDHGAALKKKGACCAGQDIHDARLAA
                     EMLGIPHYVLDYESRFRDAVIDQFADSYLAGQTPVPCIRCNQTVKFRDLLDVARDLGA
                     VAMATGHYVGRAIGPDGRAQMRKAIDPSRDQSYFLFATTPAQLDYLRFPLAGLEKPQV
                     RGVAAQLGLSIAAKPDSQDICFVPSGDYRTLIDRLRPQGREAGEIVHMDGRVLGAHAG
                     ITDYTIGQRRGLNVAVGEPLFVTKLDPQLRRVIVGPREALLTASLTLDETNWLGDQAD
                     IEAAAAAETPVLARVRSTRPPSPARLTLVDGVISVVFQTGEEGVAPGQACALYDPADP
                     DRLLGGGFIASTGAVVAV"
     gene            complement(8644..9402)
                     /locus_tag="VF633_13895"
     CDS             complement(8644..9402)
                     /locus_tag="VF633_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8472199.1"
                     /translation="MSEPSGTLIGNPVSPYVRKIMAICAMKRIDVAIDPITPFRGDDA
                     FAAMSPLRRIPVWIEGDVTLCDSSIIAQYLEETRPEPSVLPHHPRDRARARWIEEYAD
                     TRLFDVLGWKLFFQIAVKPRILKEPTDGSAVEHARAVEIPQTFEYLETVMPQAGFLFG
                     EAPGLADFSLAPAFLNARVVGVEVDAGRWPLLAAWLGRVEDETPLKKLNEVAMAFMTT
                     KLADQPAMMAEHGYALAVRSHDGGRAMRGPMSPV"
     gene            complement(9399..9902)
                     /locus_tag="VF633_13900"
     CDS             complement(9399..9902)
                     /locus_tag="VF633_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8472200.1"
                     /translation="MGRTADYSRQSCSIAATLEVIGDPWTLLVIRDAFQGLTRFEQWQ
                     ERLGVARNVLAARLKTLVQHGVLEPRLYSERPPRNEYILTAKGLALSDVLITMHGWGS
                     QHLYGDADSGVSFTHKTCGAELKPRLACSCCGEIVKRRDTEIHFHADRPTVAEILPKE
                     AVTKEAA"
     gene            10083..10964
                     /locus_tag="VF633_13905"
     CDS             10083..10964
                     /locus_tag="VF633_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8472201.1"
                     /translation="MTLQSPTLQTPTLQTLADALTLTSGPEGLTCSLHGDFGNGPIAA
                     PPEKGFPFGGLLAGLCANALRQGLELTAPLRSLSIQYLAAPTYEAPIVFTPRLLRGGR
                     TVAYTSLEVHQGERLTHHAQATYGDDDLNAVRVPGSIVPPPPLDSLDPARTLHGPLAP
                     RFSQHVEYRLETGPHILQGVPGDKAGERLWMRTNDGAALDEARLCYLMDALYPPAWTA
                     VTTPFRAATVDLRYDFITDPTPETAPDGLAFFEFSLVDLGGGWTMDDLIVRDGIGRIL
                     ALGRQRRKLAPTRPPKA"
BASE COUNT         2009 a         3826 c         3314 g         1971 t
ORIGIN      
        1 ttccagcatc agggtgttga tgttttcagt cgccaaggct cccgcgatgt tcgctcgcgc
       61 gccaatgacg gcgtctccga tctggttcaa cgccagatcc tgactttcga gccggtcggc
      121 gacggccgtc cccgtgtcga tgaaaccctg gacgcggttc tgcgaagcct tgagcgccgt
      181 cagactttcg gcgccgcgcc cgaagccttc catgtcggta gcgaccttct gcgtcgatag
      241 ccgggtctgg gcctcgaact gctggttctg cgccgccatg aggttgagca gcgccgattg
      301 ataatttccg gcggtggaga cgcggttcat catgtcattc ccagaagcgt gtcgtacatg
      361 tccttgaccg cctggataag gcgggccgag gcgttgaagg actgttggaa ggtcgtcata
      421 ttgaccagct cctcgtccag attgacgccc tcgtaagcgt tctggcgccc attggcctcg
      481 gtctggacgg cagtggctcc agcggcgcgg ctcttggcgg ccgaggcccg ggagccgatg
      541 tccccggaca aatcggaagc gtatcgggag accgactggg ctccgcccgc cgacccgccg
      601 cctgcctgga aattgacgga acgcttgccg gcatctgcca gtccgagcgc ccctcggcca
      661 tcgccggaac tcagtgccgt cgttcccggc gtcgctgaca ggttgagctg cgccaaggca
      721 agcttcgagg ggttcgcgat gatgtcggcc cggacgttga atccgtcggc gcgagcagct
      781 cggatcccgc cgataccgaa caactcggtc atcgacacac ccgatggcgt ttgcgtcgtc
      841 gtgtcctgga ttacgctgac cactgtggcc ggaccggaat tgcccttgaa agtcaccgct
      901 ccgccgggat ccaggacgaa ggacccgtaa cgaccgaccc ctgtaacggg attgttcatg
      961 gcggtcagaa gttcggacat cgtgcccgtt cccgtcggca cggccactgt caggtcgcgc
     1021 atccgcgtcc cgtcaggttc ggtgaaacgc agcgaaatcg tctgacccgg agaaaaccca
     1081 tgcagcgacg tgggggtcag cccgatgtcg taggtcgcgg tccgatcggt cgtgatcaga
     1141 tcgttcatgc caaaataatg cgaaaaaccg cgacccgcct tggcgctcgg cgacgtagcg
     1201 tcgtcagcga tgacgacgcc gttggtcccg gcggaggaca gtgtcagttt cccgtcggtg
     1261 aaactcgcgg tagccgcgcc ccccagttgt gtgttcagcg tggtcaggaa ggtcgaggga
     1321 tccgccgcga ccccgtttat cgtcatcgtc gatcccgcga aaacgatctt cgccgaagtc
     1381 gcgatggctc cggtggtcgg gttggtcagg gcgatcgttg ttgtgccggt gaaaccggtc
     1441 agcgccgtct ccagcgactg tcccacattt tttccggtca gggtcgtcgg cgccggaacg
     1501 ctggagttcg cgttgtgcgc ccgattcagc tcgtcggcca cgtgagccat cagttcgccc
     1561 agacgctcgg cggctgccgg ggcctcgacg tcgcgcagtt ccagcagccc cttgatttcg
     1621 ccggtgagca gccccgcgtt cagggaccgt ccctggccgc caggctcggt gacatagata
     1681 tcgttgaact tgctttcggc gttgaccgat ccggcggcgc gataatcgag cgtcgcggcg
     1741 ccggcgcccg ccaacaggat gcctgcgctg gtccggattt ccacgcctcc gttatttcgc
     1801 tgtgaaacct tgacgtccat caaggcggac aactggccga taagcgtcgc ctggttggtt
     1861 tgggagcccg aagcgtcgcc gctggtcgcc gtggcctttg caatatcgcc gttcagggct
     1921 tcgatctgct gcagaaggtc gttggccttc tcgaccgccg ttttgatccg gccgtcggcg
     1981 tcctcgcgca cggtctggat ctgggttgat atccgcgagg actctgcaaa caaggattcg
     2041 gtcttgaaca gggcttcctg acgcaagggc gaagaggtgg cgttttccgc agtgacggcg
     2101 aaggccgaaa agacgctgtc gatctgcgaa aagaagccgt cgtcccctcc cggatcgccg
     2161 aacaagctct gaatctgatc gtacagttca tatcggacgt tctggcgact cgcttccgcg
     2221 ttggcgttga agctcgccgc ctgcaggaac cgatcggtcg ccagacgaat gcgcgcgata
     2281 ccaacgcccg agcccatgcc ctccgtcgtc aacgaggcct ggtcagcgat cttgcggacg
     2341 tagccgacgg tgttgacgtt cgcgacgttg tcggaaacga tccgcagctg ggtttgggcc
     2401 gtcgcaagac cgctgctggc gatgttgagg atagagttta gcgacatagg cgtttctctc
     2461 ccttcacgaa gagggcgccc ggcaagccct gcccgcctga gcgcgttttt tgcccggcca
     2521 cgatgacgtc ctcgcgtacc aaagcgttga aacctatgac aatcgtggat cgcgcctgaa
     2581 tcatgatgcg tgaggaacgc aagcctcggg ccagtctgaa agcggacaca ggctcgctcc
     2641 cggagccggg caaaaaacga cggcggcgcg gcaagtttcg acctcgaccg ttaccggtct
     2701 cgggcatcta ctcgaatata gtttcgtctt ttccgtggct tgggtgctga tctgaaactg
     2761 gcccggcgat tgcgagggct gcttcgaatg tcccaggcgc agcaggcgcc gaccgcccca
     2821 agccagagct gcctgcctcc gcatgtcccc cgtcaccctg acatccgttc ccgtcgcgcc
     2881 gaccgccccg tcccgggcgg cgaaggctgc cgacccggct tcggctgatg cctttggcgc
     2941 catggtcgcg gacatcatgg ctgagggcgg cgacgaaggg gtcagcgcgg ctaaagacgt
     3001 ccccgcgaaa acctcgcctg agactgcagc ggaccaaaca accggcaaac ccgccgatga
     3061 agacgccgcc cagatcgaag cgagccagga caaggcgagc ccccaagtgt tgaacgcccc
     3121 gctcctgtta atggtccagc cgccagcgcc gacaacggac gggccagaga ccgccggcgc
     3181 aatcgaaact gcgccccccg caggcgtgag acccgatggc gctctgctgg cggatgctgg
     3241 aagcaccagc tccgcaccca tcgacgcctc ggaaagtttt gcgccgcctg tttccgcatc
     3301 gacgactcca gcttccggcg ctgaccccgc cccgcccccg accaggagcg acccgtccac
     3361 gaacgctcgc actgccgctg ccactgccac caccacgacc accaccgcca ccgccaccgc
     3421 cactgccact gccgctgccg ccgccgccgc tacccccgcc gctggcgtcg ctggcgccgc
     3481 tggcgccgta agccctcagg ccaagggcag tcctgtagcg gccgcgccag gctatggggc
     3541 gccggcctcg gggccgggcc tcgccctgaa gaccgacgct caagcagagg caggcgagga
     3601 cagcgagcct ctaatctcga caccgcgctc ttcgtcgcaa acttcggtga acagggctcc
     3661 gacggaccac cctgcgggtc tgccctcccg caccgtcgga ggcgccgacg cccaaacgac
     3721 atcggctccg aacgctgcag cccaatcgcc ggaaaaatcc aatgcggcga cgatggatgt
     3781 gtcacaggcc gctcctgcac cggaggacgg ttcccccgac gccccgtccc tgcttgcctc
     3841 ttcttcgacc ggccaacttt cggggggcca atccgccccc ccgtcatcgc cgacaacagg
     3901 aacctcgacc ttcgccgctc aagtgcaggc ccagtcccat tccgcggacg ccgtcctgac
     3961 agcgacgatc gacaccgcac cgaccggcca gagtcagccg gccccgcaga catcgtcgat
     4021 ctctaccatg ccggtcgtcg caagcttctc gggcctgtcg cgagcgacac ttgagaccac
     4081 cgtgcagatc gcggcgcaga tcacgcgcca actcgccggt cgttcgacgc ggttcgagat
     4141 gggcctgacg ccagaggggc tcggcaaggt caatgtcagc atggacatcg acgccgatgg
     4201 ccagctgacc gcgcgtctcg ccttcgacaa tcccctggcg gccgccgacc tgcgtgcccg
     4261 cgtcgacgaa ctgcgcggtc agcttcagga cgccggcttc aagctggccg atgacgccct
     4321 gaccttctcg catcaagatg cttcatcccg ggatggcggc gccgccgagc agcgccggga
     4381 ccagaaccag ggtcgcgcct tcgccgccgc ctcgcgcatg gcggatgacg ccgacaccac
     4441 ttcgcccgtc cccgcctggg tatccctgtc cctcacgcct cgcggcgtcg atatgaaggt
     4501 ctgaaccgat ggtcgccgcc gtcactacac caacaaccgc tgcagccaat accgacgccg
     4561 ccaagacggg tctggcgacc aatttcgaca ccttcctgac tctgctgacc gcccagttga
     4621 aaaatcagga tccgctggcg ccgatggact cgagccagtt cacccagcag ctgacccaga
     4681 tgaccggggt tcaacagcag ttgctgacca acgacctgct caccagcctg ctggcggccc
     4741 agcagggcgg tggtctcggc gacgcatcaa actacattgg caaggatgtt actgccgtct
     4801 ggtcggccga caagctgacc gatggcaagg ccgaatggag ctacgaactc ggggccgacg
     4861 cggcagccgc ttcgctgtct gtgctggaca gcgcgggcaa ggttgtctgg accggccccg
     4921 cgacggatct caaaaccggc acgcacgact tctcgtggaa cggcaagacg acggctggcg
     4981 gccagttgtc ggacggcggc gtctacacgc tgaaggtcag cgccaccaac gctgcgggca
     5041 aagccgtcga cagccaggtc ctgatgaccg gcaaggtcac cggcgtcgag ctctatgaca
     5101 acgtccccta cctgcacgtc ggcaacacca tcatcccgct gagcaccgtc atctcgcttc
     5161 agcaagcagc ggccgcgaca acgcctacca ccagtgtggt cgaccaggcc gtcgacgccc
     5221 tgtcctcggc cgcctccgct ctcaatcccc tcaaacttct ctcgtaagga tctctcgcca
     5281 tgagcatcaa cagcgccctt ctggccggag tctccggcct caccgctaac gccgcagccc
     5341 tcgccgccat ttcgcagaac atcgcgaacg tgaacactgt cggctataag cgcacggccg
     5401 ccgaattctc cactgtcgtc aacagccaga ccaacggaac aggtttttct gccggcggcg
     5461 ttcaggcgac agctcgcaac tatgtcagcc agggcggcca actcctgcgc actgcctcga
     5521 gcactgacct tggcatagat ggcagcggat tcttcgtggt caccgagcag gcggaaaatt
     5581 tgacaccgac agatacccgc atgttcaccc gcgccggcgc attcaccccc gacaagaacg
     5641 gctttctacg gaacacggcc ggtctttacc tgcagggatg gccgatcgac tcgaacggca
     5701 acatcaatac cgacccatcc gatctgaacc gcctgcggtc catcaacatc ggcgcagtcg
     5761 gcggaacggc agagccgacc acgcgcatcc agatcaacgc caacctggac gatagccaaa
     5821 gggacaacat ttccatcgcg gcaaaggacg ccgccttggc cgttcccggc ccccttgcct
     5881 acaatactgg cgccgcgaac atggctcaat acgacgagac ggccgcgagc ccgatcggga
     5941 ccaagccaga cttccgcatg actgtgcctg tatccgactc caaaggcgga cagcgcaccg
     6001 tcgccatctc gttcctgaaa agcgcaacgg ccaacgaatg gtacgccgag gttcacgccg
     6061 tacctgcaac cagcgtcgag accggcgcag ggctttcgaa tggccagctc gccaccggcc
     6121 gcgttctgtt cactcaggac ggacggctgg atatcgccac gatgaaggcc gccaacttgg
     6181 cgagcgccgg ttctgggcct tacctgttcg atgatttcga gaccgcttcg atcacgctgg
     6241 gagcctcgga ctccggtacg ccagcagctg gaatggcgaa atgggacgca gcctccggtg
     6301 tcggggcaca aaccatctcg ttcgacctta acacctcggc aggcggccta agccagttga
     6361 acactgcatc cggtgtgctg tcgaccgtca ccaacggcac tgcattcggc aacctcagcg
     6421 ccatcaagat tgacaaggaa ggcttcgtca cagccagttt cgacaacggt gtgacccgcc
     6481 ggatcgccca actggcgatc ggtactttca ccagctccga caatctgctc gccgtcagcg
     6541 gcaacgccta tcaggtcagc caaggctccg gcacgttcaa cctgaaggtc gcgggagccg
     6601 gtggagcggg agagatcgcg tcgtcccaac tcgaagcctc gaccgtggat ctctccaccg
     6661 agtttacggg tctgatcacc acccagagag cctattcggc gtcctccaag atcatcacca
     6721 ccgccgatga aatgctcgct gaactcatca gcatcaaacg ctgatcgtcg ggtaagtaat
     6781 cattagcttc aatgaatcgt tgctcaacat tcggcctaca agcctctgtg gatgaagtgg
     6841 attttatcat ttccttcacc acgacgctta aacggccctt agcggcgtct gactattgtc
     6901 acatcaacgg tgagtggtga tgaggcaaaa gccaatgttg caagagcgac gtctgaataa
     6961 caaaggcgaa caatacgtgg tcggtccaac tggcacgccc ctgacgctgc gggatcttcc
     7021 gccgccgaat accgaccgct gggtgatccg ccgcaaggcc gaggtcgtcg ccgctgttcg
     7081 cggtggcctc ctgagccttg aggacgcctt gtcgcaatat cgcctaacgg cggaagagtt
     7141 catcgcctgg caaaaggcga tcgacaaatg gggcatgcaa gggctgcgca ccacgcgcat
     7201 ccagagctac cgctcctagg gttttcccta tcaatggaat ccaacggccg ctcccggcaa
     7261 cgggcgcggc cgttgccgtt ttgaggatga acttctggct catctgtgcc gagacctcga
     7321 aattccgtcc agtcggcgct atatgccctt catgaccgcc gtagcctcgc tcccggcaga
     7381 tgatttctgc gtccttcccg agatcgccac cgctgacatg gatcaggcgg tcgaggccgc
     7441 acgtctggcg gtcggcctgc ccgtcgggtc gcggatcgtg gccgccatgt caggcggggt
     7501 cgactctacg gtcgtcgcgg cgctgctgca caaggcgggc tacgacgtcg ttggagtgac
     7561 gcttcaactt tacgaccatg gcgcggcgct gaagaagaag ggcgcctgct gcgctggtca
     7621 ggacatccat gacgcccgtc tggcggccga aatgctcggc atcccgcact acgtactcga
     7681 ctacgaaagc aggttccgtg acgcggtgat cgaccaattc gcggactcct atctggcggg
     7741 ccagacgccg gtcccgtgca tccgttgcaa ccagacggtc aagttccgcg atctgctgga
     7801 tgtggcccgc gatctcggcg ccgtggccat ggccaccggc cactatgtcg gccgcgccat
     7861 cggcccggac ggtcgggccc agatgcgcaa ggcgatcgac ccgtcgcgcg accagtccta
     7921 cttcctgttc gccaccaccc ccgcacagct cgactatctg cgcttccccc tcgccggtct
     7981 cgaaaagccg caggtgcgcg gcgtggccgc ccaactcggc ctgagcatcg cggccaagcc
     8041 cgacagccag gacatctgct tcgtgccgtc cggcgactac cggaccctga tcgaccgtct
     8101 gcgcccgcag ggtcgtgagg ccggagagat cgtgcacatg gacgggcgtg tgcttggcgc
     8161 ccacgccggc atcaccgact acaccatcgg ccagcggcgg ggtctgaacg tcgccgtggg
     8221 cgagcccctg ttcgtcacga aactggatcc tcaactgcgc cgcgttatcg ttgggccgcg
     8281 cgaagccctg ctgacagcct cactgactct ggacgagacc aactggcttg gcgaccaggc
     8341 cgatatcgaa gcggcggccg cagccgaaac gccggtactc gcgcgtgtcc gctcgacccg
     8401 tccgccgtcc cctgcccggt tgacgcttgt tgacggcgtc atcagcgtcg ttttccagac
     8461 gggcgaagaa ggcgtcgctc ccggtcaggc ctgcgccctc tacgaccccg ccgatcccga
     8521 ccgcttgctg ggcggcggct tcatcgcctc gaccggggct gtcgtcgccg tctgacggcc
     8581 agaggtacat cggccgcgac ggacatcgtg gccctgacta cctctacccg gacagccccc
     8641 tgcctagacg ggcgacattg gcccgcgcat cgcccgcccg ccgtcgtggg accgtacagc
     8701 cagcgcatag ccgtgttcgg ccatcatcgc gggttgatca gcaagcttcg tcgtcatgaa
     8761 agccattgcg acctcattca gcttcttcag cggggtctcg tcctccaccc tgcccagcca
     8821 ggccgcaagc agtggccagc gtccggcatc gacctccacg ccgaccaccc gcgcattcaa
     8881 gaaggcgggc gcaaggctga agtcggccaa tcccggcgct tcgccgaaca ggaagcccgc
     8941 ctgcggcatc acggtctcga ggtattcaaa ggtttgggga atctcgactg ccctggcgtg
     9001 ctcgaccgcg ctcccgtccg tcggctcctt caggatgcgc ggcttgacgg cgatctgaaa
     9061 gaacagcttc cagcccagca catcgaacag ccgggtgtcg gcgtattcct cgatccagcg
     9121 cgcgcgggcc cggtccctcg gatggtgcgg caggaccgat ggctccggac gcgtctcctc
     9181 aagatattga gcgataatgc tggaatcgca cagcgtcaca tcgccttcga tccacaccgg
     9241 gatacgcctc agagggctca tcgcggcaaa ggcgtcatcg ccgcgaaaag gggtgatcgg
     9301 gtcgatcgcg acgtcgatcc gcttcatcgc gcagatcgcc atgatcttgc gcacataggg
     9361 cgagaccgga ttaccgatca gggttcccga aggctcgctc acgccgcctc tttcgtcacg
     9421 gcttccttgg gcaggatttc ggcgaccgtt ggacggtcgg cgtggaaatg aatctcggtg
     9481 tcgcgccgtt tcacgatctc gccgcaacag gaacaggcca gccgcggctt cagttccgca
     9541 ccgcaggtct tgtgcgtgaa gctcacgcct gagtcggcgt ctccatacag atgctgcgag
     9601 ccccagccgt gcatggtgat caacacgtcg gacagggcaa ggcccttggc ggtgaggata
     9661 tattcgttgc gcggcgggcg ttcggaatag agacgcggct caagcacgcc gtgctgaacc
     9721 agcgtcttga gccttgcggc cagaacattg cgcgcaacgc ccagccgctc ctgccactgc
     9781 tcgaaccggg tcaggccctg aaaggcgtcc cggatgacca ggagggtcca cggatccccg
     9841 atgacctcca gcgttgcggc gatcgaacag ctttgtctgg aatagtctgc ggtgcggccc
     9901 atgcagggaa agtgaccttg cgtcactcat tttgcaagtg ggttgccaaa ccgaacgctc
     9961 taagtcagtc tcgtttggct acggacatgt cgttcttgcg gcgacggttc ctgcattcct
    10021 cgactgaaca cgggcggagg cgacgtcttc cgcccgtctc ctttggacgc taaccaagcc
    10081 tcatgacctt gcagagcccg accttgcaaa cgccaacgct gcaaacattg gcagacgccc
    10141 tgaccctgac gtccggcccc gagggcctga cgtgctcgct gcatggcgat ttcggcaacg
    10201 gccccatcgc cgcaccgccg gaaaaaggct tcccgttcgg cggccttctg gcgggacttt
    10261 gcgcaaacgc cctgcgtcag ggcctcgaac tcaccgcacc cttacgcagc ctgtcgattc
    10321 aatatctggc ggctccgacg tatgaagcgc ccatcgtctt tacccctcgc ctgctgcgcg
    10381 gtggtcggac cgtcgcttac acatcgcttg aggtgcatca gggcgagcgg ctcacccacc
    10441 acgcccaggc gacctacggc gacgatgatc tcaacgccgt ccgcgtgccc ggctccatcg
    10501 ttccgccgcc accgctggac agcctcgatc ccgcccgcac cctgcacggt ccccttgccc
    10561 cgcgcttttc ccagcacgtc gaataccggc tcgagaccgg gcctcacatc ctgcaaggcg
    10621 tgccgggcga caaggcggga gagcggctgt ggatgcggac aaacgacggg gccgcgctag
    10681 acgaggcgcg gctctgttat ctgatggacg ccctttaccc gcctgcatgg acggcggtga
    10741 cgaccccgtt ccgggccgcg accgtggacc tacgctacga cttcatcacc gatcccacgc
    10801 ccgagactgc gcccgacggc ttggccttct tcgagttctc gctcgtcgat ttgggcggcg
    10861 gctggaccat ggacgacctg atcgtgcgag acgggatcgg gcgtatcctc gccctcggcc
    10921 gccagcgccg taaactggcc ccgacgcgtc cgccgaaagc ctgatcggca ctgggcagaa
    10981 cggccgttga ccctgccgtc tgccgctccg cttgaacccg accctcagac caccgatcta
    11041 gaccgcaaca gtttcccttc aggacaccat ccgtgaccgc ttcaaacgat cctatcgtca
    11101 tcgtctcgtt cgcccgcacg
//
LOCUS       DASVTM010000504         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_898698,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000504 DASVTM010000000
VERSION     DASVTM010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898698"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..594)
                     /locus_tag="VF633_13910"
     CDS             complement(163..594)
                     /locus_tag="VF633_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472202.1"
                     /translation="MRAISGATSSIRAGILTMRGLASVVILSAALALAACGDPAPAKQ
                     AEAPATVAEQTAVMPEDAPPQEETEAPVEEASAEGPASSCLADIGATASAKLVDRCIM
                     VSPATHPPCNAQNECAMIQGEIDRSCAMYGPNETRPAECAA"
     gene            complement(950..1678)
                     /gene="rph"
                     /locus_tag="VF633_13915"
     CDS             complement(950..1678)
                     /gene="rph"
                     /locus_tag="VF633_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX8472203.1"
                     /translation="MRPSDRTPEQIRTVTLETGVNRYAEGSCLVSFGHTKVLCTASIE
                     DKVPGWMRNTGQGWVTAEYGMLPRATHTRGRREAAAGKQSGRTQEIQRLIGRSLRAVV
                     DLKALGERQVLIDCDVIQADGGTRTASITGAWVAMATALGYLKSEGVLKVDPILDQVA
                     AVSCGVFENEPVLDLDYEEDSNAEADSNFVLTGSGTIVEIQATGEKRGFSRAEFDRLF
                     QLAEIGCADLFVMQREALAIALKR"
     gene            1730..>2193
                     /gene="hrcA"
                     /locus_tag="VF633_13920"
     CDS             1730..>2193
                     /gene="hrcA"
                     /locus_tag="VF633_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267629.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX8472204.1"
                     /translation="MYAPNPLPFGTAPLGSGAGLTALDQRARDIFRRIVETYLENGEP
                     VGSRTLSQRGVSLSPASIRNTMQDLTMAGLLAAPHSSAGRIPTHAGLRLFVDGFLEIG
                     DIGQDERREIDARLAGKGRDFDEALDAASSLLSGLAGGAGIVASPVSDAGVK"
BASE COUNT          338 a          762 c          716 g          377 t
ORIGIN      
        1 cgctcagcat ggggttcacc cccttctatc aggccgacgg cggcctgctg ccgtgggtgc
       61 gcgaccatgt cctgggcaag gtcgcgcgcc tgccgatcgc gccccggctg ctggcggcga
      121 cggtgtctgg cgtgttgctg gacccgcgac acgcctgatc gatcaggcgg cgcattccgc
      181 tggcctggtt tcgttcggtc cgtacatggc gcacgagcgg tcgatctcgc cctgaatcat
      241 cgcgcactcg ttctgggcgt tgcacggcgg atgggtcgca gggctgacca tgatgcagcg
      301 gtcgaccagc ttcgccgatg cggtcgcccc gatgtctgcc aggcacgaac tcgcaggacc
      361 ttcagccgaa gcctcctcaa ccggcgcctc ggtctcttcc tgcggcgggg catcctcggg
      421 catgacggct gtctgctctg ctaccgtcgc cggcgcctcg gcctgcttag ccggagccgg
      481 atcgccacag gccgccagcg ccagagccgc cgacaggatc accacagaag ccaatccacg
      541 catcgtcaaa atccccgccc tgatcgagct cgtcgcgccc gagatggcgc gcacgatgcc
      601 ccgatccgcc cttaagcgat agtgggggcg accgggggct gcggggcccg gcgagtggag
      661 actctcgacc ctgatactct cgacactttg agccccccga atggagacac tgaccccttt
      721 gccgcgcaat cggtccggcg gtggccatgg cgatggtaga ctgactgtgg agacgggctg
      781 caacacgccg cccgcaccca ccgatctttg acagcctcat ctccgtcgtc tccgtctcag
      841 gagatgacct tcacgctgcg ccttcgcggc gtgcgctcga ttgcgcgagc cctcctatgg
      901 agggttcaac ccgccgatta ttccgggact ggagacactc gacccggtgc taccgcttga
      961 gcgcgatcgc cagcgcttct cgctgcatca cgaacaggtc ggcgcaaccg atctcggcca
     1021 actggaacag gcggtcgaac tcggcgcgcg agaagccgcg tttctcgccg gtggcctgaa
     1081 tctcgacgat cgtgcccgag ccggtgagca cgaaattgct gtcggcctcg gcgttggagt
     1141 cttcctcgta gtcgaggtcc agcacgggct cgttctcgaa cacgccgcag gacaccgccg
     1201 cgacctgatc gaggatcgga tcgaccttca gcacgccttc ggacttcaga tagccgagcg
     1261 ccgtcgccat cgcgacccag gcgccggtga tcgaggcggt ccgcgtgccg ccgtcggcct
     1321 gaatgacgtc gcagtcgatc agcacctgac gctcgcccag cgccttcaga tcgacgacgg
     1381 cgcgtagcga acggccgatc agacgctgga tttcctgcgt acggcccgat tgcttgcccg
     1441 cagcggcctc acggcgaccg cgcgtatggg tcgcgcgagg cagcatgccg tattcggcgg
     1501 tgacccagcc ctgaccggta ttgcgcatcc agcccgggac cttgtcctcg atcgaggcgg
     1561 tgcacaggac cttggtatgg ccgaacgaga cgaggcagga gccctcggcg tagcggttga
     1621 cgccggtttc cagcgtcacg gtgcggatct gttcaggcgt acgatcggag gggcgcatat
     1681 caatgtccta gcaagcgaga gcggcgctgc ttatcctgaa agcgtgagcc tgtatgcccc
     1741 caacccgctt ccgttcggca cagcccctct tgggagcggc gctggcctga ctgcgctgga
     1801 ccagcgcgcc cgcgacatct tccgccgcat cgtcgagacc tatctggaga acggcgaacc
     1861 ggtcgggtcg cggaccctgt cccagcgcgg cgtctccctg tcgccggcct cgatccgcaa
     1921 caccatgcag gacctgacga tggcgggctt gctggcggcc ccgcactcgt cggccgggcg
     1981 gatcccgacc cacgcgggtc tgcgcctgtt cgtcgacggc ttccttgaga tcggcgacat
     2041 cggtcaggac gaacgccgcg agatcgatgc gcggctggcc ggcaagggcc gcgacttcga
     2101 cgaggcgctg gatgcggcgt cgtcattgct gtccggcctg gcgggaggcg ccggcatcgt
     2161 cgcctcgccg gtcagcgacg caggtgtgaa aca
//
LOCUS       DASVTM010000505         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_898886,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000505 DASVTM010000000
VERSION     DASVTM010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898886"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VF633_13925"
     CDS             complement(<1..1388)
                     /locus_tag="VF633_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEX8472205.1"
                     /translation="MTSTAATPAAITGTLRLVLGDQLSDNLSALDGLDPARDVVLMAE
                     VRDEATYVRHHKQKIALVFAAMRSFALRLEARGVTVRYVHIDDPDNTQGIVGELHRAL
                     DDQPFANVVITECGEWRLAEALAAFGGVATVPVDIREDHRFICTHDRFRRWASGKSQL
                     RMEFFYREMRKVTGILLDDGQPVGGKWNYDAENRRKLTKGLRPPARLRTPPDAIAAEA
                     IADVARLFPEHFGDLDAFGWPTTAEQAETALADFLANVLPGFGDWQDAMARGEPWMWH
                     GLISTSINLRLLDPLDVCRRAEAEYRAGRSPLNAVEGFIRQILGWREFVRGIYWLKVP
                     EYRQRNFLDADRELPWFYWSGETDMACVADVVEMTRAHAYAHHIQRLMVTGNLAMLLG
                     VHPDAIDDWYMVVYADAYEWVEMPNTRGMATFADGGIMGTKPYAASGAYIDRMSDYCR
                     GCRYDVKQKSGE"
     gene            1394..2227
                     /gene="sseA"
                     /locus_tag="VF633_13930"
     CDS             1394..2227
                     /gene="sseA"
                     /locus_tag="VF633_13930"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEX8472206.1"
                     /translation="MTSSPLISTTELAALLNDPGLRIIDGSWHLDGRDAAAEHLAGRI
                     PGAVRFDLEAVSDHSTDLPHMLPTPEAFAEAVGALGVSEGDTVVIYDSVGLFSAPRVW
                     WTFRLMGAKNVRVLDGGLPQWLAEERAIESGPVAAATPARFTPTPRTRDVADLTAILS
                     ALTGDAQIVDARPAARFNGEAPEPRAGLRSGHMPGALNLPSKSLLTEDGRLRRGDDLS
                     ARFAEVGLDLDGPIITSCGSGVTAAILTLALAELGRDSALYDGSWAEWGGRTDTPVAL
                     G"
     gene            complement(2250..3572)
                     /locus_tag="VF633_13935"
     CDS             complement(2250..3572)
                     /locus_tag="VF633_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8472207.1"
                     /translation="MTETKRGGIPLHWLMLIGFVVGLGGGLAVNMLVGGDTPWVIWLT
                     TNITGPAGQIFLRLLFMLVIPLLFSALVVGVAEMGDLKSLGRAGIKTLLLTILVSGIA
                     VLIGIGMVNLFRPGDGVDPLLAQSLLAQGAGGAAKIVENAPTTIKLGDFFLDLIPSNV
                     FTAASENQILPVMVFALFFGIGLVMAKSPSTDRLQQVIEGILEVTMKLINMVIKLAPY
                     AIACLMFNLAALFGWDLLVRLAAYVGVAVGAMLIHMFVVYPLVVWIGGGMNPVTFFKG
                     IREPMVVAFSTASSNAALPVSLKAAEEQLHLPRKIARFVLTVGATANQNGTALFEGVT
                     VLFLAQFFNIDLSLTQQIVVMLVCILGGVGTAGVPAGSLPVVAMILVMVGVPAEGIGL
                     ILGVDRFLDMCRTTLNVTGDLVLATVVSRGETDAPMLAGEEEPVAPGV"
BASE COUNT          670 a         1231 c         1297 g          598 t
ORIGIN      
        1 tcctcgccag acttctgctt cacgtcgtac cgacaccccc ggcagtagtc gctcatccgg
       61 tcgatgtaag cgcccgaggc ggcgtagggc ttcgtcccca tgatcccgcc gtcggcgaag
      121 gtcgccatgc cgcgcgtgtt cggcatctcc acccactcat aggcgtcggc gtagacgacc
      181 atataccagt cgtcgatcgc gtccggatgg acgcccagca gcatggcgag gttgccggtg
      241 accatcagcc gctggatgtg gtgggcatag gcatgggcgc gggtcatctc gaccacgtcg
      301 gcgacgcagg ccatgtcggt ctcgcccgac cagtagaacc acggcagctc gcggtcggcg
      361 tcgaggaagt tgcgctgccg gtattcaggg accttgagcc agtagatgcc gcgcacgaac
      421 tcgcgccagc cgaggatctg gcggatgaag ccctcgaccg cattcagagg ggagcgaccg
      481 gcgcggtact cggcctcggc gcggcggcag acgtccagcg gatcgagcag gcggagattg
      541 atcgaggtcg agataagtcc gtgccacatc cacggctcgc cccgcgccat agcatcctgc
      601 cagtcgccga agcccggaag cacgttggcg aggaagtcgg ccaaagccgt ctcggcctgt
      661 tcggcggtgg tcggccaacc gaaggcgtcg aggtcgccga aatgctcggg gaacaggcgg
      721 gctacgtcgg cgatggcctc agcggcgatg gcatcgggcg gcgtgcgcag gcgggcgggt
      781 gggcgcagtc ccttggtcag cttccgccgg ttctcggcgt catagttcca tttgccgccg
      841 accggctggc catcgtcgag cagaatgccg gtgaccttgc gcatctcgcg gtagaagaac
      901 tccatgcgca actggctctt gcccgaggcc cagcggcgga atcggtcatg ggtgcagatg
      961 aaccggtgat cctcgcggat gtcgaccggg acggtggcca cgcccccaaa cgccgccaat
     1021 gcctcagcca gccgccattc gccgcattcg gtaatgacga cattcgcaaa cggctgatcg
     1081 tcaagcgcgc ggtgcagttc gccgacgatg ccctgggtgt tgtcggggtc gtcgatgtgg
     1141 acatagcgga ccgtcacgcc gcgcgcctca agccgcaggg cgaagctgcg catggcggcg
     1201 aaaaccagcg cgattttctg cttgtgatgg cggacatagg tcgcctcgtc gcgcacctcg
     1261 gccatcagga ccacgtcacg cgcggggtcg agaccgtcca gcgcagacag gttgtcggac
     1321 agctggtcgc cgagcaccag gcggagggtt ccggtgatcg ccgctggcgt ggctgccgtg
     1381 ctagtcatcc atcatgacct cttccccgct gatctccacg accgaactcg ccgcgctgct
     1441 gaacgatccg ggcctgcgca tcatcgacgg cagctggcat ctggacggtc gcgacgctgc
     1501 ggccgagcat ctggccggac gcataccggg cgcggtgcgg ttcgatctgg aggcggtgtc
     1561 cgaccattcg accgacctgc cgcacatgct gccgacgccg gaagcgttcg ccgaggccgt
     1621 gggcgcgctg ggagtgtcgg agggcgacac ggtggtgatc tacgacagcg tagggctgtt
     1681 ctctgcgccg cgcgtctggt ggaccttccg gctcatgggg gcgaagaatg tgcgggtgct
     1741 cgacggcggg ttgccgcaat ggctggcgga agagcgtgca atcgagagcg ggccggtagc
     1801 ggcggcgacg ccagcccgct ttacgcctac gccccgcacc cgcgatgtcg ccgacctgac
     1861 ggccatcctc tcagccctga ccggggacgc gcagatcgtg gacgcccgcc ccgccgcacg
     1921 gttcaacggc gaagcgcctg agcctcgcgc gggtttgcgg tctggccata tgcccggcgc
     1981 gctcaacctg ccgtccaaaa gcctgctgac cgaggatggt cggctacggc gtggcgacga
     2041 cctgtccgcg cgattcgccg aggtcgggct cgatctggac ggtcccatca tcaccagttg
     2101 cggctcaggc gtcaccgccg ccatcctgac gctggcgctg gccgaactcg gccgcgacag
     2161 cgccctgtac gacggttcgt gggcggaatg gggtggacga accgatacgc cggtggcgct
     2221 gggttagacc ccagacttaa acgccagagc tagacccccg gcgcgaccgg ctcttcttcg
     2281 cccgccagca tcggcgcatc ggtttcgccg cgtgacacga ccgtggccag caccagatcg
     2341 ccggtgacgt tcagggtggt gcggcacatg tcgaggaagc ggtcgacgcc gaggatgagg
     2401 ccgatgcctt cggccggcac gccgaccatg accaggatca tcgccacgac cggcagcgag
     2461 cctgcaggca cgcccgccgt gccgacgccg ccgaggatgc agaccagcat caccacgatc
     2521 tgctgcgtca gcgacaggtc gatgttgaag aactgggcga ggaacaggac ggtgacgccc
     2581 tcgaacagcg cggtgccgtt ctggttggcg gtggcgccga cggtcagcac gaagcgggcg
     2641 atcttgcgcg gcagatggag ttgttcctcg gcggccttca gcgacaccgg cagggcggcg
     2701 ttggacgagg cggtcgagaa ggcgacgacc atcggctcac ggatgccctt gaagaaggtg
     2761 acggggttca tgccgccgcc gatccagacg accagcggat agaccacgaa catgtggatc
     2821 agcatcgcgc cgacggcgac gccaacatag gccgcgagcc gcaccagcag gtcccagcca
     2881 aacagggcgg cgaggttgaa catcaaacag gcgatcgcat agggcgccag tttgatgacc
     2941 atattgatga gcttcatggt cacttcgagg atgccctcga tgacctgttg cagccggtcc
     3001 gtggatggcg acttggccat caccagtccg atgccgaaga acagggcgaa gaccatcacc
     3061 ggcaggatct ggttctcgga cgcggcggtg aagacgttgg acgggatcag gtcgaggaag
     3121 aagtcgccca gcttgatggt ggtaggcgcg ttctcgacga tcttggccgc cccgcctgcg
     3181 ccttgggcca gcagggattg ggccagcaac ggatcgacgc cgtcgccggg ccggaacagg
     3241 ttgaccattc ctatgccaat gagcacggcg atgcccgaga cgaggatggt cagcaacagc
     3301 gtcttgatgc cggcgcggcc gagggatttg aggtcgccca tctcggccac cccgaccacg
     3361 agggccgaga acagcagcgg gatgaccagc atgaacagca ggcgcaggaa aatctggccc
     3421 gccggccccg tgatgttggt ggtgagccag atgacccaag gcgtgtcgcc gccgaccagc
     3481 atgttgacgg cgagaccgcc gccgagaccg accacgaagc cgatcagcat cagccagtga
     3541 agcgggatcc cgccacgttt cgtctcagtc attcgtcccc acgaggtcgg tgtgtcgggc
     3601 gcagcaacag cgccggtgat cgatcagcgg atcggcaggc cgtagatcag gccgccgggt
     3661 cgcgcattcc attgtgcatt caatccgcgc gccaggtcga gcgccgattg cgtacccaga
     3721 ttccgttcga atatctcacc gtagttgccg accccgacga cggcgtccag cgcccagtct
     3781 ttcgacagcc cgagcc
//
LOCUS       DASVTM010000506         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_579883,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000506 DASVTM010000000
VERSION     DASVTM010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579883"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VF633_13940"
     CDS             complement(<1..355)
                     /locus_tag="VF633_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HEX8472208.1"
                     /translation="MGAVGLQTHIWANNTRSVILLAMFPVLLVGLIFGVQLVAMGLGV
                     LPNSGRGLGDDMAYAASMLGSTIPLAIVVAGVWFAIAWFGNQAIMDAMTGAKKVERRD
                     MPELYNLLENLSISRG"
     gene            complement(378..947)
                     /locus_tag="VF633_13945"
     CDS             complement(378..947)
                     /locus_tag="VF633_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8472209.1"
                     /translation="MLTLIIIVVIIAVVGFMVVGGYNRLVALNQKADQSFADIDVQLK
                     QRNDLIPNLVETVKGYASHESGTLQAVTAARAAAAGATTVNDKVQADNMLTGALGRLF
                     AVAEAYPDLKANTNFLELQRELSDIENKLAAARRFFNNAVAEFNAVRAQFPTVLFAGM
                     FGFGADKPFFAVDAADRAAMTAAPPSVKF"
     gene            complement(1041..>1528)
                     /locus_tag="VF633_13950"
     CDS             complement(1041..>1528)
                     /locus_tag="VF633_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX8472210.1"
                     /translation="GRGHGEPLRLMIELEEKADIIAVLTYQAGSKLLVASKAGYGFIA
                     PEDDMLAQKRGGKQVLNGDLLAILRLGAADHIAVIGENLKTLIFKAEELPEMGRGKGV
                     KLQSYKQGGLQDIAAFNGEAGPEWVDGGGRRRNWPDWKDWLGKRAGSGRMGPRGLRKF
                     R"
BASE COUNT          257 a          518 c          495 g          258 t
ORIGIN      
        1 ggccgcgcga gatcgagagg ttctcgagca ggttatagag ctcgggcatg tcccggcgct
       61 cgaccttctt cgccccggtc atggcgtcca tgatggcctg attgccgaac caggcgatgg
      121 cgaaccagac gcccgcgacc acgatcgcca gcgggatggt cgagcccagc atcgacgcgg
      181 cataggccat atcgtcgccc agcccgcgcc cggagttggg cagcacgccc agccccatgg
      241 cgaccagctg gacgccgaag atcaggccga ccagcagcac cgggaacatg gccagcagga
      301 tgaccgaccg ggtgttattg gcccagatgt gggtctggag accgacggct cccatgccat
      361 gaagctccga acgggcccta gaacttcacc gaaggcggcg cggcggtcat ggccgcacga
      421 tccgcagcgt cgacggcgaa gaacggcttg tccgcgccga agccgaacat cccggcgaac
      481 aggacggtcg ggaactgggc ccgcaccgca ttgaactcgg cgacggcgtt gttgaagaag
      541 cggcgggcgg cggccagctt gttctcgatg tcggatagct cgcgctgcag ttcgaggaag
      601 ttggtgttgg ccttgaggtc gggataggcc tcggcgaccg cgaacagccg acccagcgcc
      661 ccggtcagca tgttgtcggc ctgaaccttg tcgttgaccg tcgtggcccc ggcggcggcg
      721 gcgcgagcgg cggtcaccgc ctgcagggtg ccgctctcat gcgaggcata gcccttcacc
      781 gtctcgacca ggttggggat caggtcgttg cgctgcttca gctgcacgtc gatgtccgcg
      841 aacgactggt ccgccttctg gttcaacgcc accagccggt tgtagccgcc cacgaccata
      901 aagccgacca cggcgatgat gacgacgatg atgatcagtg tgagcatgag gacctcccaa
      961 acgatgcttg agtattgcgc ggggtgggag cgacggaaag cgataatgtc ccctctcccg
     1021 ccgggagagg gtagccctcg ctacctgaac ttccgcagtc ctctcggacc catccgaccg
     1081 gaccctgccc gcttgccgag ccagtccttc cagtcgggcc aattgcggcg gcgaccgccg
     1141 ccgtcgaccc attcgggacc ggcttcgccg ttgaaggccg cgatgtcctg cagcccgccc
     1201 tgcttgtagg actggagctt gacgcccttg ccgcggccca tctcgggcag ttcctcggcc
     1261 ttgaagatca gcgtcttgag gttctcgccg atgaccgcga tgtggtccgc cgcgcccagc
     1321 cgcaggatgg ccagcaggtc gccgttcagc acctgtttgc cgccgcgctt ctgggccagc
     1381 atgtcgtcct cgggagcgat gaagccatag cccgccttcg acgccaccag cagcttcgag
     1441 cctgcctgat aggtcaggac ggcgatgatg tcggccttct cctccagctc gatcatcagc
     1501 cgcagcggct cgccatgccc ccttcccg
//
LOCUS       DASVTM010000507        10278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_635187,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000507 DASVTM010000000
VERSION     DASVTM010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635187"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VF633_13955"
     CDS             <1..1372
                     /locus_tag="VF633_13955"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HEX8472211.1"
                     /translation="GWVEHDAVEIWAATLQTCREVIRKAGGVSRFAAIGITNQRETAV
                     IWDAATGEPLHNAIVWQDRRTADVCAGLAAEGHEPMVQAATGLILDPYFSATKFAWLL
                     DKVPGARERAERGEVKLGTIDAWLMWKLTAGQSHATDATNASRTSLMDLKTRQWRDDL
                     CELFDVPKTALPRIVGCSEISGESDPALFGRALPIAGSAGDQQSALVGHGALEPGDAK
                     ITYGTGAFLVANVGDEPVGSSSRLLGTLGYQVGETVAPTVAYALEGSIFSAGSAIQWL
                     RDGVGMISESRHSEALAQGLTDNGGVYLVPGFTGLGAPWWKPDARGAVIGLTRDSKPA
                     HFVRAALEALAYQTRDLLDALATDGAPRLLVLKVDGGVTANAFAMQFVADICDVTVER
                     PAFQEMTALGAAKLAALGVGLIEDLKVRTTDAPAVWKPRMAESERERLLKGWRKAVQA
                     AIIAAD"
     gene            1423..2502
                     /locus_tag="VF633_13960"
     CDS             1423..2502
                     /locus_tag="VF633_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX8472212.1"
                     /translation="MTSPAIEDPQSAFSGTREVDPRYALDEAKLGEWLAANVDGFEGP
                     LTIRQFKGGQSNPTYELVTPGKSYVLRRKPPGVLLPSAHAVDREFTVISALSKQDYPV
                     ARPYALCTDDAVIGSMFYVMDKVEGRILWDLKLPGMDPSERRAIYEAQTDALAALHAF
                     DPSAIGLGDYGKPGNYFERQVGRWTKQYRASEIDPIPAMDRLIAFLPSTLPPEGPTRI
                     VHGDFRLDNLIMEEEQPRVKAVLDWELSTLGDPMADFSYLLIGWVLPSTVRNGIGGLD
                     LESLGIPSMDETAERYAAKAGIAKPGNLDWMMAYNLFRLAAICQGIAGRVRDGTAASA
                     HARTMAAQVPALSAAAWSFAVKAGA"
     gene            complement(2506..3258)
                     /locus_tag="VF633_13965"
     CDS             complement(2506..3258)
                     /locus_tag="VF633_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX8472213.1"
                     /translation="MKFRGWIIGAAVLVAGTTTMGSSAQPAASRAAAPVRRAVPTEPV
                     VVELFTAQGCPGCPDANQVVETIAEEPGVIALTYAVGYWDYMGWPDTFAKPQFAQRQR
                     AYQTAMRLRGVYTPQVIIDGRRQVSGAEAPAVQTAVDEEAARRDFPPQIEFRESGDRV
                     GVGSGRPPAGGAEVWAVIYKPGAQMVTVNGGDNRGRVVRHVNVVRDLRKLGDWTGRPV
                     LYALPAHREADEAVAIMVQAKRDRRILNAATL"
     gene            complement(3346..4521)
                     /locus_tag="VF633_13970"
     CDS             complement(3346..4521)
                     /locus_tag="VF633_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX8472214.1"
                     /translation="MRFLSPAALSLVLAGCVSTAPAVPNLPPIVSPGADSGSNTGPRP
                     YPVPSSPGSAAASSPTRLAGWDGEDFVAAFNAYLRGCAVSRDPASARQCARALDLQRT
                     SRPVTPSLARTFFETGFSIVAAQTPDGRDGLLTSYFAPEYSASHVRTDEFDMPVLARP
                     LDLERTSGGVVQRRLDGSTGPYADRAAIEAAAAAGSPSAPVLGWMRAEDLFFLQIQGS
                     GYLTFEDGSLSRAAYAADNGLKFVGIARPMEQQGLLPRNGTSGEAIRTWLAAHRGREA
                     RSITALNPRYIFFDLQPDDGGNPAGAAGVPLPPRRAIAVDPASWTYGDLVWIDADGGN
                     LAGAHTSYRGLVMALDTGSAIRGPVRADFYMGRGQRAGAEAGTVRHPLRMWRLVPKP"
     gene            complement(4554..5192)
                     /gene="timA"
                     /locus_tag="VF633_13975"
     CDS             complement(4554..5192)
                     /gene="timA"
                     /locus_tag="VF633_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-related membrane protein TimA"
                     /protein_id="HEX8472215.1"
                     /translation="MPVQFQILIFAGIAAFVLFQLYNVLGKRVGRQPQDDARAPALPS
                     ADTTAPVRPALDSITMAAISGLRARDPQFDPQKFLDGSRQAYETIVRGYSSGDRAALK
                     PLLAPAVMESFETGIVARETRGETEQVEFLHAPRADLESATVEGDKAIAKVRFLAEIR
                     SKILLPEGEATPGAPAEPRIEDRRTAEHWTFERTLGASDPNWVLARVEPANA"
     gene            5414..5923
                     /gene="secB"
                     /locus_tag="VF633_13980"
     CDS             5414..5923
                     /gene="secB"
                     /locus_tag="VF633_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX8472216.1"
                     /translation="MTDVSTNPADAAQAQDGTPPNGGFRILAQFVRDLSFENPKAPDS
                     LRIEGKPAIDLGVELNAQGRPDGLFEVDMKLSVKATTDAMTVFHIELLYGGLFQLNNV
                     APADIEPLLLIECPRYLFPFAREIIARATADGGFYPPFMMDPIDFVAIYMARQQQLAA
                     NSDAGTGQA"
     gene            complement(5956..6693)
                     /gene="dnaQ"
                     /locus_tag="VF633_13985"
     CDS             complement(5956..6693)
                     /gene="dnaQ"
                     /locus_tag="VF633_13985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX8472217.1"
                     /translation="MAREIVLDTETTGFDPRTGDRLIEVGCIEIHDLLPTGRTFHRYC
                     NPERLIPEDAIRVHGITDDKVKDAPKFSAIVADLMDFIGDAPVIAHNAQFDRNFIDHE
                     CGRCGQKLLHETRWIDTLQLAQKRWPGMPNSLDALCKRYKVSLADRSFHGALIDARLL
                     AEVYLELRGGKERVLDLTSRRGAEQAAAFAAAGGHGLRPRPLGLRSTDEEHDRHLAFL
                     TATLKDRSLWQAYGLTTEPDMEKASAG"
     gene            complement(6751..7362)
                     /gene="coaE"
                     /locus_tag="VF633_13990"
     CDS             complement(6751..7362)
                     /gene="coaE"
                     /locus_tag="VF633_13990"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263609.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX8472218.1"
                     /translation="MILLGLTGSIGMGKSTTAGMFADEGAVVWNADDAVHQLYSVGGA
                     AVEPVGEAFPGVVVDGAVDRARLADALGRDDAAFKRLEAIVHPLVAAGRAGELAEAAE
                     RGVKLAVLDIPLLFEGGGQTAFHAVVVVTADAQTQAQRVLARPGMTRERFEAILERQM
                     PDAEKRRRADFIVETGRGLEVARDQVRAIVATVLDPDWISPHR"
     gene            complement(7362..8201)
                     /locus_tag="VF633_13995"
     CDS             complement(7362..8201)
                     /locus_tag="VF633_13995"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX8472219.1"
                     /translation="MNRHNITGGATLAGIVGQPVAHSLSPAIHNAWLAEAEIDGAYVG
                     FGPADEAAFYVLFAAGKAGLIRGFNVTAPFKGQALALADTATDTARLCGSANTVVFEN
                     GKAHADSFDGQGLMEALHEQAPALSVVGKPVVVLGAGGAARAGAAALSTAGAEVRILN
                     RTSERAEILAAHLGDLVTVADGPEEAFPGAVLVVNALSAPPAFDMDFLPPGIVLMDMS
                     YKPVITPFLAAGRARGLTVVDGLAMLVGQARPSFEALFGQAPPKLDVRAVALNSLRKR
                     GEA"
     gene            complement(8198..8803)
                     /locus_tag="VF633_14000"
     CDS             complement(8198..8803)
                     /locus_tag="VF633_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX8472220.1"
                     /translation="MSEHREERLILASRSAARRAMLTDAGVSFGLEDAGVDEDAIKAA
                     MADASPEALAIELACAKALAVSRRDPDVWVLGADQTLAFDGGLISKAKSLEEARTRLK
                     SMRGRSHQLHSGAALATKGQIVWSGVDTVQMKMRDFSDAFLDGYLAAEGNEVLACVGS
                     YRLEGLGSQLFEAVDGDYFTVLGLPLWPVLAELRRAGVIKT"
     gene            complement(8800..9696)
                     /locus_tag="VF633_14005"
     CDS             complement(8800..9696)
                     /locus_tag="VF633_14005"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX8472221.1"
                     /translation="MTPFSPTGKPVGPAKSGPLRLATYFHVHLVSDSTGETLNAMAKA
                     VTARFEGVIPIEHIYSLVRSEKQMQRVLEEITTAPGVVLHTLVDRDLRAQLEEGCRAI
                     DTPQIGALDPLVGALSRYLGAALSTRVGAQHALDHEYFNRISALDYAMAHDDGQGTSE
                     QLEGADVVLCGVSRTSKTPTCIYLAHRGIRAANVPLVPGQEDGERLVGLKNPLVIGLT
                     VSPDRLVQIRRNRLEGLNADYSSDYVDHDLVRDETVKARRAFERRGWPTIDVTRRSVE
                     ETAAAIVNLLSEKRSRRLGVAW"
BASE COUNT         1766 a         3440 c         3369 g         1703 t
ORIGIN      
        1 cggctgggtc gagcatgacg ccgtcgaaat ctgggccgcg accctgcaga cctgtcgcga
       61 ggtgatccgc aaagccggag gcgtgtcgcg gttcgccgcc atcggcatca cgaaccagcg
      121 cgagacagcg gtgatctggg acgctgcgac cggcgagccc ctgcacaacg ccatcgtctg
      181 gcaggatcgt cgcaccgccg acgtctgcgc cgggctcgcc gccgaggggc atgaaccgat
      241 ggtgcaggcg gcgaccggcc tgatcctcga tccctatttc tcggcgacca aattcgcctg
      301 gctgctcgac aaggtcccgg gcgcgcgaga gcgggcggag cgcggcgagg tcaagctggg
      361 caccatagac gcctggctga tgtggaaact gactgcgggt cagagccacg ccaccgacgc
      421 cacaaacgcc agccgaacct cgctgatgga cctcaagacc cgacagtggc gcgatgacct
      481 gtgcgaactg ttcgatgtgc ccaagaccgc cctgccccgc atcgtcggct gctccgaaat
      541 cagcggcgaa agcgacccgg ccctgttcgg tcgcgccctg ccgatcgcgg gttcggctgg
      601 cgatcagcag tcggcgctcg tcggacacgg tgcgctggaa cccggagatg cgaagatcac
      661 ctatggcacc ggcgccttcc tcgtcgccaa cgtcggggat gaaccggtcg gctcatcaag
      721 ccgcctgctg ggcacgctcg gttatcaggt cggtgagacc gtggccccca ccgtggcgta
      781 tgcgctggag ggttcgatct tttcggcggg atcggccatt cagtggctgc gggatggcgt
      841 cggtatgatc tcggagtcac ggcactccga ggccttggct cagggactga cggacaatgg
      901 cggggtgtat ctggtgcccg gcttcaccgg tctgggcgcg ccctggtgga agcccgacgc
      961 acgcggcgcc gtcatcggcc tgacccggga ttcaaagccc gcccatttcg tccgggccgc
     1021 actggaagcc ctcgcctatc aaacgcggga tttgctggat gcgctggcca cggacggcgc
     1081 gccgcgcctg ttggtgctca aggtcgacgg cggggtgacg gccaacgcct tcgccatgca
     1141 gtttgtcgcc gacatctgcg atgtcacggt cgagcggccc gcctttcagg aaatgacggc
     1201 gctgggcgcg gccaagctgg cggccttggg tgtcgggctg atcgaggacc tcaaggttcg
     1261 caccacggac gcgccggccg tctggaaacc gcgcatggcg gagagcgaac gcgaacgtct
     1321 tttgaagggc tggcgcaaag ccgttcaggc cgccatcatc gccgccgact gagccagaag
     1381 agcgcccgca aagggcgccg caacggaaac accgaggacc gcatgaccag cccggcgatc
     1441 gaagacccac aatccgcctt ctccggcacg cgcgaggtcg acccccgtta tgccctcgac
     1501 gaggcgaaac tgggcgagtg gctggcggct aatgtggacg gtttcgaagg tccgctgacg
     1561 atccgccagt tcaagggcgg ccagtcgaac ccgacctatg aactggtcac gccgggcaaa
     1621 tcctatgtct tgcgtcgcaa gccgcccggt gtgctgctgc cctcggcgca cgccgtggat
     1681 cgcgagttca cggtgatctc ggctctgtcg aaacaggatt atccagtcgc cagaccctat
     1741 gctctgtgta cggacgatgc cgtgatcggc tcgatgttct acgtcatgga caaggtcgaa
     1801 ggccggatcc tgtgggacct gaagctgccc ggcatggacc cttcggagcg ccgcgccatc
     1861 tatgaggccc agaccgatgc actcgccgcc ctgcatgctt tcgatccgtc agccataggc
     1921 ctcggcgact acggcaagcc tggcaactat ttcgaacggc aggtcgggcg ctggaccaag
     1981 cagtatcgcg cctcggagat cgacccgatc ccggccatgg accggctgat cgccttcctg
     2041 ccctccaccc tgccgccgga agggccgacg cggatcgtcc acggcgactt ccgtctcgac
     2101 aacctgatca tggaggagga acagccccgg gttaaggccg tgctggactg ggagttgtcg
     2161 acgctcggcg atccgatggc ggatttctcc tatctgctga tcggctgggt cctgccctcg
     2221 acggtgcgga acggcatcgg cggtctcgat ctggagtcgc taggcatccc ctcaatggac
     2281 gagacggccg aacgctatgc ggccaaggcg ggcatcgcca agccggggaa cctcgactgg
     2341 atgatggcct acaatctgtt ccggctggcc gcgatctgtc agggcattgc aggccgcgtc
     2401 cgcgacggca ccgccgccag cgcccacgcc cggaccatgg cggcgcaggt ccctgccctg
     2461 tcagcggcgg catggagttt cgccgtcaag gcgggcgcct gacgcttaga gcgtggcggc
     2521 gttgaggatg cggcggtccc tcttggcctg aaccatgatc gcgacggcct cgtcggcttc
     2581 acggtgcgcc ggcaaggcgt agagcaccgg acgcccggtc cagtcgccga gcttgcgcaa
     2641 gtctcggacg acgttgacgt ggcgcacgac ccgaccgcga ttgtcgccgc cgttaacggt
     2701 caccatctgc gcgcccggct tgtagatcac cgcccagacc tcggccccgc ctgcgggagg
     2761 ccggcccgat ccgacaccga cgcgatcacc gctctcacgg aactcgatct gcggcggaaa
     2821 gtcacgacgc gcagcctctt catctacggc ggtctgtacg gctggagcct cggcgcccga
     2881 cacctgacga cgaccgtcga tgatcacctg tggcgtatag acgccgcgca gccgcatcgc
     2941 cgtctggtag gcgcgctggc gttgagcgaa ctgcggtttg gcgaaggtgt cgggccagcc
     3001 catgtagtcc cagtaaccca cggcgtaggt cagggcgatc acgcccggct cctcggcgat
     3061 ggtctcgacc acctgattgg cgtccgggca tccggggcag ccctgtgcgg tgaacagttc
     3121 aaccacgacg ggttcggttg ggacggcgcg ccgaacggga gcggctgcgc gcgacgccgc
     3181 aggctgggcc gaggacccca tggtcgtcgt ccccgcaacc agcaccgccg cgccgatgat
     3241 ccatcctctg aacttcatgc ggcgaaccgt agcgatggat cctcccgacc gccgctcata
     3301 tctgcgtgag cccagaaatg aaccgttgag ggtcagggcc aggactcagg gtttgggaac
     3361 cagccgccac atccgcagag gatgccgaac tgtcccagct tcggcgcccg ccctctggcc
     3421 acggcccatg tagaaatcgg cccggacggg tccccggatg gccgacccgg tatccagcgc
     3481 catgacgagt ccgcgatagg aggtatgggc gccggccaga tttccgccgt cggcgtcgat
     3541 ccagaccaga tcgccatagg tccagctggc ggggtcgacc gcgatcgcgc gtcgaggggg
     3601 caggggcacg cccgccgctc cggctgggtt gccgccgtcg tccggctgca ggtcgaagaa
     3661 gatgtagcgc gggttcaggg cggtgatcga tcgcgcctcg cggcctctat gcgcagcgag
     3721 ccaggtccgg atcgcctcgc cggaggtgcc gttgcgcggc agcaaaccct gctgttccat
     3781 cggtcgggcg atgccgacga atttcaggcc gttgtcggcg gcgtaggcgg cgcgcgacag
     3841 gctgccgtcc tcgaatgtca gatagcccga gccctggatc tgcaggaaga acaggtcttc
     3901 ggcccgcatc cagcccagca ccggcgcaga cggcgaaccc gccgccgctg cagcctcgat
     3961 ggcggcgcgg tcggcatagg gacccgtcga gccgtccaag cgacgctgga ccacgcctcc
     4021 ggacgtgcgc tccagatcaa gcgggcgcgc cagcaccggc atgtcgaact cgtccgtccg
     4081 gacgtggctg gctgaatatt cgggggcgaa atagctggtc agaagaccat cccgcccgtc
     4141 cggagtttgc gccgcaacga tgctgaaccc ggtctcgaag aaggtgcggg cgagggatgg
     4201 cgtgacgggc cgcgaggtgc gctggagatc gagcgcccga gcgcattgac gagcgctggc
     4261 cgggtcacgg cttaccgcgc agccccggag gtaggcgtta aaggccgcga cgaaatcctc
     4321 gccgtcccaa ccggccagcc gtgtcgggct gctggcggct gcggaaccgg gggaggaggg
     4381 aaccggatac ggcctcggtc cagtattgga cccgctatca gcgccgggcg acacgatcgg
     4441 cgggaggttc ggcacagcgg gcgccgtgct gacgcagccg gccagcacca acgaaagagc
     4501 tgcgggcgaa agaaagcgca agccgaccct cgccaaccgc tgccgccaga acatcaggcg
     4561 ttggccggct cgacccgggc caggacccag ttgggatcgg aggcgcccag cgtgcgctcg
     4621 aacgtccagt gttcagcggt gcgacggtcc tcgatgcgcg gctcggcagg cgcaccgggt
     4681 gtagcctcgc cttcgggcag aaggatcttg ctgcgaattt ccgccaggaa gcgaaccttg
     4741 gcgatggcct tgtcgccttc gacggtggcg ctttccagat cggcgcgggg ggcgtgcagg
     4801 aattcgacct gctcagtctc gccacgggtt tcgcgggcga cgatgccggt ctcgaaggac
     4861 tccatcacgg ccggagccag caagggcttc agcgcggccc ggtcgcccga ggaatagccg
     4921 cgcacgatcg tctcataggc ctgacgcgaa ccgtcgagga atttctgcgg atcgaactgc
     4981 ggatcgcggg cgcgcaggcc cgagatggcg gccatggtga tggaatccag cgccggacga
     5041 accggggctg tcgtgtcggc cgaaggcagg gctggcgcgc gggcgtcatc ctgcggctgg
     5101 cgaccgaccc gcttgcccag cacattatag agctggaaca gcacgaaggc ggctataccc
     5161 gcgaagatca agatctgaaa ctggaccggc aagatgatat cctgggaatg gcgccgcgcc
     5221 tcaccggacc acgggaccgg aacgcgcgat gaacgattag ggtctcatat agggaggcgc
     5281 aaggcgagac gcacccggtg acatgccctg acaggcgcgc gccgttgccg cacgcccgtt
     5341 cggcgtgtta gtcgcgcgcc ttcaccgggt cgcgacagac ctccggtttc actccattca
     5401 cggcccttcg cccatgaccg atgtttcgac caaccccgcc gacgccgccc aggcccagga
     5461 cggaaccccg cccaacggtg gcttccgcat cctggcgcaa ttcgtccgcg acctatcctt
     5521 cgagaacccc aaggcccctg acagcctgcg tatcgagggc aagcccgcca tcgacctcgg
     5581 cgtcgaactg aacgcacagg gtcgtcccga tggcctgttc gaggtcgaca tgaagctctc
     5641 ggtcaaggcc accacggacg ccatgacggt gttccatatc gaactgctgt acggcggcct
     5701 gttccagctg aacaacgtcg ctccagccga catcgagccg ctgctgctga tcgaatgccc
     5761 gcgctacctg ttcccgttcg cccgcgagat catcgcccgc gccacggcgg acggcggctt
     5821 ctacccgccc ttcatgatgg acccgatcga cttcgtggcc atctacatgg ctcgccagca
     5881 gcaactggcc gccaattcgg atgccggtac cggtcaagcc taagctttag aggcccgaac
     5941 cttgaaaggc gtccgtcagc cggcggacgc cttttccatg tccggttcag tggtcaggcc
     6001 ataagcctgc cacagcgaac gatccttcag cgtcgcagtc agaaaggcga gatgccggtc
     6061 gtgttcctcg tctgtggaac gcagacccaa gggccgagga cgcaggccat gaccaccggc
     6121 tgcagcaaag gcggcggcct gttccgcgcc ccggcggctg gtcaggtcga gaacccgctc
     6181 cttgccgccg cgcagctcga gataaacctc ggccaacagg cgggcatcga tcagggcgcc
     6241 gtggaacgag cggtcggcga ggctgacttt gtagcgttta caaagggcgt ccagcgagtt
     6301 cggcatgccc ggccagcgct tctgggccag ttgcagcgtg tcgatccagc gggtctcgtg
     6361 cagcagtttc tgaccgcaac gcccgcattc gtgatcgatg aagttcctgt cgaactgggc
     6421 gttgtgagcg atcaccggag cgtcgccgat gaaatccatc aggtccgcga caattgccga
     6481 gaatttcggg gcgtccttga ccttgtcgtc ggtgatgccg tggacccgga tggcgtcctc
     6541 ggggatcagg cgttcgggat tgcagtagcg gtggaacgtt cggcccgtcg gtagcaggtc
     6601 gtggatctcg atacagccga cctcgatcaa cctgtcgccc gtgcgagggt cgaaacccgt
     6661 ggtttcggtg tcgagtacga tttcgcgggc catgggccct taatggggtg tttcgagcct
     6721 cggcgaaaga gtcgccgagg tcagcacggt tcagcgatgg ggagaaatcc agtcgggatc
     6781 gagcacggtg gccacgattg cgcgcacctg gtcccgagcg acttccagcc cgcgaccggt
     6841 ctcgacgatg aagtcggccc gccggcgctt ttccgcgtcc ggcatttgcc gttcgagaat
     6901 ggcttcgaac cgttcgcggg tcatcccggg ccgcgccaat acccgctgcg cctgcgtctg
     6961 cgcgtcggca gtgaccacga ctacagcgtg aaaggcggtc tggccgccgc cctcaaacag
     7021 cagaggaata tccagcaccg ccagtttcac gccgcgttcg gcggcctcgg ccaactcacc
     7081 ggcgcggcct gcagcaacca gcgggtggac gatggcctca agccgtttga acgccgcatc
     7141 gtcccgaccc agcgcgtcgg ccagccgcgc ccgatccaca gcgccgtcca cgacgacgcc
     7201 gggaaacgcc tcgcccaccg gttcgaccgc cgctccgcct acagaataga gctggtgcac
     7261 cgcgtcgtcg gcgttccaga ccactgcgcc ttcgtcagcg aacatcccgg ccgtcgtcga
     7321 tttgcccatg ccgatcgagc cggtgaggcc gagcaggatc atcaggcctc gccacgcttg
     7381 cggagggagt tcagggccac ggcgcgcaca tccagtttcg gcggagcctg cccaaacaac
     7441 gcttcgaatg acggtcgcgc ctgaccgacc agcatggcca agccatccac aaccgtcagc
     7501 ccccgcgccc ggcccgccgc gaggaagggc gtgatcactg gcttgtagct catgtccatc
     7561 aggacgatgc cgggtggcag aaaatccatg tcgaaagctg gcggcgcgct cagggcgttg
     7621 accaccagaa ccgcgccggg aaacgcctcc tctggtccgt cggcgaccgt aacgagatcg
     7681 ccaaggtgcg cggccagtat ctcggcccgt tcgctcgtcc gattgagaat tcgcacctca
     7741 gccccggctg ttgacagggc ggcggccccg gcccgggccg caccgcctgc gcccagcacc
     7801 acgaccggct ttccgacgac gctgagtgcg ggagcctgtt cgtgcagggc ctccatcaga
     7861 ccctgcccgt cgaaactgtc ggcgtgcgcc ttgccgttct cgaacacgac cgtattggcc
     7921 gatccgcaca gccgcgccgt gtcggtcgcg gtgtcggcga gtgcgagggc ttgtcctttg
     7981 aacggagccg tgacgttgaa gccccggatc agcccggcct tgcccgcggc gaacagcaca
     8041 tagaaggccg cctcgtccgc cgggccgaac cccacgtaag cgccgtcgat ctcggcctcg
     8101 gctagccagg cgttgtggat ggcgggactg agtgaatgag cgacgggctg gccgacgatg
     8161 ccagcaagcg tggcgccgcc agtgatgttg tgtcggttca cgtcttgatc actcccgcgc
     8221 gcctcagttc cgccagcacc ggccacaggg gcaggccgag gacggtgaaa tagtcgccat
     8281 cgaccgcctc gaacaactgc gagcccaggc cttccagccg gtatgagccg acgcaggcga
     8341 gaacttcatt cccttccgcg gccagatacc catcgaggaa ggcgtcggag aaatcgcgca
     8401 tcttcatctg cacggtgtct accccggacc agacgatctg gcccttcgtc gccaaggcgg
     8461 cgccggaatg cagttggtgc gagcgaccgc gcatggattt cagccgagtc cgggcctctt
     8521 cgagcgattt cgccttggag atcagacccc catcgaaggc cagggtctgg tctgcgccca
     8581 gaacccacac gtccggatca cgccgcgaca ccgccaaagc cttggcgcag gccagttcga
     8641 ttgcgagcgc ttcaggactg gcgtcggcca tcgccgcctt gatcgcgtcc tcgtccaccc
     8701 ccgcgtcctc gagcccaaag ctcacaccgg cgtcggtcag catcgcccga cgggcagcgc
     8761 tgcgcgaggc caggatcagc ctctcttctc tgtgctcact caccaggcca ctcccagccg
     8821 acggctgcgc ttttcgctca gcaggttgac gatggcggca gccgtttcct cgaccgaacg
     8881 gcgggtcacg tcgatagtcg gccagcctcg ccgctcgaac gcccgacgcg ccttgaccgt
     8941 ctcgtcgcgc accagatcat gatccacata gtccgagcta tagtcggcat tgaggccttc
     9001 cagccggttg cgccggatct gcaccagacg gtccggagaa acggtcagcc cgatgaccag
     9061 cgggttcttg agccccacca gccgttcgcc gtcctcctga cccggcacca gcggcacatt
     9121 ggcggcgcgg atgccgcggt gcgccagata gatgcaggtc ggggtcttgg aggtgcgcga
     9181 aaccccgcac aggacgacgt cggcgccctc aagctgttcg gaagtgccct gaccgtcgtc
     9241 gtgagccatg gcgtagtcga gcgcgctgat gcgattgaag tattcgtggt cgagcgcgtg
     9301 ctgggcgccg acccgcgtcg acagcgccgc gcccagatat cgcgacaggg cgcccaccag
     9361 aggatccagc gcgccgatct ggggtgtgtc gatggcccgg caaccttctt cgagctgagc
     9421 gcggagatcc cggtcgacca gggtgtgcaa gaccacgccc ggcgccgtgg tgatttcctc
     9481 cagcacccgc tgcatctgct tttcggatcg gaccagcgaa tagatgtgct cgatcgggat
     9541 cacgccctcg aaccgggcag ttactgcctt ggccatcgcg ttgagggttt cccccgtcga
     9601 atcggaaacc agatgcacat ggaaataggt cgccaggcgc aatgggccgg atttggccgg
     9661 tccaactggt tttccggtgg gggagaaggg tgtcatgcgc tgggactcat cgatggattc
     9721 ctgtggaaac ccagttccct tgctgggcgc cgagaccggc ggtctgggcg ccgatctcta
     9781 tagccgcgcc ctccgcccgg cgcgagcggg gagagcgggg agggtttgga tctgtcggtg
     9841 tcgattgttc caagggccgg tgggaccctg cgtcctggca gtggacaatc ttaacaaaag
     9901 gtaaacggcg cccatctgac gcgtgtcatc gatcggctgc gaggcccggc tgttaaggct
     9961 tcgttaagga ttatccccgg tttctacagg cccgtcctgc atgtcagatc agcgatggcc
    10021 gtccgtggat gttcggtttc cgttcacggc acgactgcca aacgatcccc gccgtcccgc
    10081 gccctacctc cttcttccaa tcctttttaa ttcttgatca gattaagagg agggacggtg
    10141 cgtcattgga caaaccgggg gcttgaggga accgcaggct cctcgtagga ccagcgtcat
    10201 gacaacgtct ccggtccagc ccgcactcct tcaagttctt tccggcgaga agaccgatcg
    10261 tccgcccgtc tggttcat
//
LOCUS       DASVTM010000508         4576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_315783,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000508 DASVTM010000000
VERSION     DASVTM010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315783"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VF633_14010"
     CDS             <1..147
                     /locus_tag="VF633_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX8472222.1"
                     /translation="AIGGITVENARSLADAGADFIAVSAGVWKFAHGPAAAVVAFNDE
                     LSKG"
     gene            249..1934
                     /locus_tag="VF633_14015"
     CDS             249..1934
                     /locus_tag="VF633_14015"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8472223.1"
                     /translation="MSLSRALETPTGTLAKAPTRVVARAGLSTNTTRLFSQPLVPMAT
                     VSAVLLLTLFSIRFGMSADGAVVMWVANAVGVGVWLRMGRGVAFDICFAAMMAVAVAS
                     GEFLAGYSPQKSGVLTAINVFEMVAAVTLVRRFSPDLSMQTVRSVLRLIAVAVIGAPF
                     VAACAGAIALFALKGVPMLEGFQVWWFGHSLGMLLVLPMILSLNARSLDLFRRPLRMV
                     EWVGLLGMLGAVVYGRYYLHIPVIAVFLNLLLVVIAARLRISGLTVAMLVTAVIALGA
                     LLQGQASPSAEPAGIRVMSAQLMLISVTLPFMLVAVLLSERDGLSARARAGQKRAELA
                     SEAKSRLLANVAHEIKSPVAGVIGIGDLWSSGGLGPVTATQKEMADMLVKTARQVEAL
                     AHDLLDVARAESGAVKVELRPTDVSGVAEDVRRATALRPEARNLRLEVVREGDGLIAL
                     ADSQRLSQVIDNLATNAVKYGGSGGVVTFRVSRVFEGVRIEVIDQGPGLSAEKQTQLF
                     EPFNRLGLERSTVEGHGIGLALAKRLVELQGGVIGVDSEPGQGATFWVELPAA"
     gene            2374..2859
                     /locus_tag="VF633_14020"
     CDS             2374..2859
                     /locus_tag="VF633_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472224.1"
                     /translation="MTYPAPTLPAGRAGSTAPSATSSVQPPVAAPASVEAPPPVLTVL
                     SPPAVAALPFRVELPQGFRITSGRPGPDFNIYTIRRGDQSFAMVYVGPSSQFPIYDGE
                     MVQTGGRSSIVVNEGGQRHAKEHLFQRTASPREIHVWVSSLDGADAALSERIAQSVDA
                     K"
     gene            complement(2882..3223)
                     /locus_tag="VF633_14025"
     CDS             complement(2882..3223)
                     /locus_tag="VF633_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8472225.1"
                     /translation="MREDGKIDYLEFPAGDVGTVKAFYAAAFGWTFQDYGPSYVAFSE
                     GLDGGFDGDPADKSAAPLPVLYAHDLEAMLAKVEAAGGSVVKPIYAFPGGRRFHFRDP
                     AGNELAVWSEG"
     gene            complement(3413..>4576)
                     /locus_tag="VF633_14030"
     CDS             complement(3413..>4576)
                     /locus_tag="VF633_14030"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8472226.1"
                     /translation="IVSFARTPMGGFQGVFTGVKSTELGATAVRAAVERAGIAPEKVD
                     QIYMGCVLSAGLGQAPARQAGIGAGLGQHVEATTLNKMCGSGLQAVMLASDSLAAGSA
                     DIIVAGGMESMTGAPFLMNKHRAGARIGHDVIIDSMMMDGLEDAYDAGKPMGSFAEDA
                     ARTYQFTREAQDAYAVESLTRASAAIASGAFKAEITPVEVTGRKGAVTIFEDEQPGKA
                     MPEKIPSLKPAFAKDGTITAASSASISDGAAALVLTRESVAKAMGLPIVARIVSHSAH
                     AHEPGLFTTAPVPAIQKALKKAGWSVEDVDLWEINEAFAVVPMIAMQELGMPHSKVNV
                     NGGATALGHPIGASGARVLATLLGALQARGGTKGVASLCIGGGEAVAMAVELA"
BASE COUNT          705 a         1528 c         1523 g          820 t
ORIGIN      
        1 gctatcggcg gaatcacggt ggagaacgcg cgctcactgg ccgacgccgg cgccgatttc
       61 atcgccgtca gcgccggcgt ctggaaattt gcacacggcc cggctgcagc tgtcgtcgca
      121 ttcaacgatg agctgtccaa aggttaggtt gcgggctccc aggggcctgg ttcgggtcgt
      181 taacttcact ggatattagg gagcaaagcc ttagctggcg catcgtcgct aatgtcgagt
      241 cgccgcctat gtcgctcagc cgcgcgctgg aaacccccac gggaacgctc gccaaggctc
      301 caactcgcgt cgtcgcgagg gccggcctgt cgacgaatac cacgcggctg ttcagccagc
      361 cccttgtccc catggcgaca gtcagcgcag tcctgctgct gaccctgttt tcgatccgtt
      421 tcggaatgtc cgcagacggg gcggtcgtga tgtgggtcgc caatgctgtt ggggttggcg
      481 tctggctgcg aatgggccgc ggcgtagctt tcgatatctg ttttgcggcc atgatggccg
      541 tcgcggtggc cagtggagag ttcctcgcag ggtattcgcc ccaaaagtcc ggcgtcctga
      601 cggctatcaa cgtgttcgag atggttgccg ccgtgacgct ggtgcgccgc ttctcgcccg
      661 acctgtcgat gcagacggtc cggtcggtac taagactgat cgccgtcgcg gttatcggag
      721 cgccgttcgt cgccgcttgc gcgggcgcca tcgcattgtt tgccctgaag ggcgtcccga
      781 tgctggaagg cttccaagtc tggtggttcg gtcattcgct gggcatgttg ctggtcctgc
      841 ccatgatcct gtcgttgaat gcgcggtcgc tagatctctt caggcggcca ctgcggatgg
      901 tggagtgggt cggcctgctt gggatgctgg gcgctgtcgt ctatggccgc tactatctcc
      961 acattcccgt cattgccgtt tttcttaacc tgctgctggt ggtgatcgcc gctcgtctga
     1021 gaatcagcgg cctgaccgtc gccatgctcg tcaccgccgt catcgcgtta ggcgccctgt
     1081 tgcagggcca ggcctcaccc tccgccgagc ctgccgggat ccgcgtcatg tcggcgcaac
     1141 tgatgctgat ctccgtcacc ttgcctttca tgctggtcgc agtgctgctc agcgagcgcg
     1201 acggcctgtc ggcgcgagcg cgcgccgggc aaaagcgcgc cgaattggct tcggaagcca
     1261 agtcacgcct gctggccaac gtcgcccacg agatcaaaag tcccgtcgcc ggcgtcatcg
     1321 gcatcggcga tctctggtcc agcggcgggc ttggtcccgt gacggcgacc cagaaggaaa
     1381 tggccgacat gctggtcaag accgcgcggc aggtcgaagc cctggctcat gacttgctgg
     1441 atgttgcccg cgccgagtca ggagcggtca aagttgaact gcgacccacc gatgtgtcgg
     1501 gtgtggctga ggacgttcgc cgcgccaccg ccctgcgtcc cgaggctcgc aatctgcgtc
     1561 tggaagtcgt ccgggaaggc gacggcctga tcgccctggc cgactcccag cgactgagtc
     1621 aggtgatcga caacctggcc accaacgcgg tgaaatatgg gggctcggga ggggtcgtga
     1681 ccttccgggt gtcgcgcgtc ttcgagggcg tgcggatcga ggttattgac cagggtcctg
     1741 gtctctcggc ggagaagcaa acccagttgt tcgagccgtt caaccggctg gggctggagc
     1801 gatcgacggt cgaggggcac ggcatcggtc tggcccttgc caagcgactg gtcgagcttc
     1861 agggcggggt catcggcgta gactccgaac cggggcaggg cgcgaccttc tgggtcgaac
     1921 ttccggccgc ctgatcaggt cttgccgtcg ggacgccgag aggcgatgtt ccgccgcatg
     1981 aaaaccgctc gtaccctccg cgtctcgctg ttgctcgcct cgaccgctgc catgatcgcc
     2041 ctgcctgcgt gggcgcagac aacgtcgagc cggacgccgg cgaccacgca agcggcccca
     2101 ggggcagccg ctggcagcgc cctgacccgc agcctcaacg gcggcccgcc cgcttcggcg
     2161 acgaccgccc cctcagccac gacgtctacg agagccacaa ctccagtccc aactgccgct
     2221 cccgctgcgg cccctagagc gagcacgaca cctgcgccag cgcgcgctca acccgcgccg
     2281 actgcaactg gcccggcgcc tgcttcttcg accgcccccg ctgcgacacc gcgcgcgacc
     2341 gcgcccactt caacggcgcc ggcatcagga gcgatgacct atccggcgcc gaccctgccc
     2401 gcagggcgtg ctggctcaac tgctccttcg gcgacctcct cggtccagcc gccggtagcg
     2461 gcgcctgcct cggttgaggc gccgcccccg gtcctgaccg tgctgagccc gcctgctgtg
     2521 gccgccctgc ctttcagggt cgagctgccg caagggttcc gcatcaccag cggccgcccg
     2581 ggaccagact tcaacatcta cacgatccgg cgcggcgacc agtcattcgc catggtctat
     2641 gtcgggccgt cgtcgcagtt cccgatctat gacggggaga tggtccagac cggggggcgc
     2701 tcctcgatcg tcgtcaacga aggtggccag cggcacgcga aggaacatct gttccagcgc
     2761 accgccagcc cgcgcgagat ccacgtctgg gtgtcgagcc tggatggcgc cgacgcagcg
     2821 ctcagcgaac gcatcgcgca atctgttgac gcgaagtagg gcggtcgacg ccaaataggg
     2881 tctagccttc cgaccagacc gccagttcgt tgcccgccgg gtcgcggaag tggaaacgcc
     2941 gcccgcccgg gaaggcgtag atcggcttga ccacgctgcc gcctgcggcc tcgaccttgg
     3001 ccagcatggc ttcgaggtcg tgggcgtaga ggacgggcag aggggcggct gacttgtcgg
     3061 cgggatcgcc gtcgaacccg ccgtccagac cttcgctgaa cgccacatag ctggggccgt
     3121 agtcctggaa ggtccagcca aaggctgcgg cgtagaaggc tttcaccgtg ccgacgtcac
     3181 cagcgggaaa ctcgagatag tcgatcttgc cgtcttcgcg catgttcgca ttccgttctg
     3241 aaaagccacc gaaagagggc ttgccatacg gatcaatcgc acgcaatctt gagaactatt
     3301 cgcagtcgca gagtcgcgtg atgatcgttc tgaccgccgg accttcagct ctcagtcgcc
     3361 tgatgcaggc ggatcgcaca tccgagtgcg ggtccgccct gatcaccaga cctcaggcca
     3421 gctcgaccgc catggccacg gcctcgccgc cgccgatgca caggctggcg acgcccttcg
     3481 tgccgccccg cgcctgaagc gcaccgagca gggtggccag aacgcgggcc ccggatgcgc
     3541 cgatggggtg tccgagggcg gtggcgccgc cgttcacgtt gaccttcgaa tgcggcatgc
     3601 cgagctcctg catggcgatc atcggcacga cagcgaaggc ctcgttgatt tcccacagat
     3661 cgacgtcctc gaccgaccag ccggccttct tgagggcctt ctggatcgcg gggaccggcg
     3721 cggtggtgaa caggccgggc tcgtgggcgt gggccgaatg gctgacgata cgcgcgacga
     3781 tcggcaggcc catggccttg gccacgctct cgcgggtcag gaccaaagcc gctgcgccgt
     3841 cggagatcga ggccgaggag gcggcggtga tggtgccgtc cttcgcaaag gcgggtttca
     3901 ggctggggat cttctcgggc atcgccttgc cgggctgttc gtcctcgaag atcgtgacgg
     3961 cgcctttgcg gcccgtgacc tcgaccgggg tgatctcggc cttgaacgcg cccgaggcga
     4021 tggcggcgct ggcgcgggtc aggctctcaa ccgcataggc gtcctgcgcc tcgcgcgtga
     4081 actgataggt gcgggcggcg tcctcggcga acgagcccat gggcttgccg gcgtcatagg
     4141 cgtcctccag cccgtccatc atcatggagt cgatgatgac gtcgtggccg atgcgagccc
     4201 cggcgcggtg cttgttcatc aggaacggcg cgccggtcat cgactccatg ccgcctgcga
     4261 cgatgatgtc ggcgctgccc gctgcgaggg agtcagacgc aagcatcacc gcctgaagac
     4321 ccgagccgca catcttgttc agggtcgtgg cctcgacgtg ctggccgaga ccggcgccga
     4381 ttccagcctg acgcgccggg gcctgaccga gtccggcgga caggacgcag cccatgtaga
     4441 tctggtcgac tttctccggc gcgatcccgg cgcgttcgac ggcggcgcgg acggcggtgg
     4501 cgcccagttc ggtcgacttc acgccggtga agacaccctg aaagccgccc attggcgtgc
     4561 gggcgaacga gacgat
//
LOCUS       DASVTM010000509         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_152803,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000509 DASVTM010000000
VERSION     DASVTM010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152803"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VF633_14035"
     CDS             complement(<1..371)
                     /locus_tag="VF633_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8472227.1"
                     /translation="MTDTRASTLRALLFASSAAGMLCAAPAIAKTAPDTPIATAPSDD
                     VLQEQGSNRVADVDVNGVRVRREPRSPKFVAPLVDTPRSVTVIPQELIEQIGATSLQD
                     LLRTSPGITFGAGEGGQPLADR"
     gene            599..>2019
                     /locus_tag="VF633_14040"
     CDS             599..>2019
                     /locus_tag="VF633_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX8472228.1"
                     /translation="MADPTGGMGLGHAVALLAAAVVAVPLFKRLGAGAVLGYLAAGLV
                     IGPFGFKLIQDAEAVLHVAELGVVMFLFIIGLEMRPARLWSLRKEIFGLGALQVLTCG
                     ALLTGAALVAGLSVPAAIIAGAGFALSSTAIVMQLLEERNEQSDPAGQRVVSVLLFED
                     LSIVPLLALVAVFGGMFGTAIENPQPIWLTVGFAIAAVASVYVIGKWVMNPVLRLLAR
                     YGGREIMTAAALLVVTGAAWAMDLGGLSMAMGAFLAGVLLSESTFRHQLEADVEPFRG
                     ILLGLFFLSVGMSLDLGVVVRDWQLVVGGIAAFMFAKMAGVYAIARIKSGHGEALKRA
                     ALMGEGGEFAFVLYGAALAAGLFDPRSAAILSAIVILSMALTPVRMLLADRFKPEDVL
                     SMEGIDEAHGLRERVLIIGFGRFAQVVAQPLLSRDVDVSIVDIDVEMIQAAGKFGFKV
                     YYGDGARLDVLRASGAGTAETVLV"
BASE COUNT          283 a          602 c          729 g          405 t
ORIGIN      
        1 cggtcggcca gcggctggcc gccctcgcct gcgccgaatg tgatgcctgg cgaggtgcgc
       61 agcaggtcct gcagcgacgt cgcgccgatc tgctctatga gttcttgcgg gatcaccgtg
      121 acgctgcgcg gcgtatcgac cagcggggcg acgaacttgg gtgatcttgg ctcgcggcga
      181 acgcgcacgc cgttgacatc gacgtccgcg acgcggttcg aaccctgttc ctgaagcaca
      241 tcatcgctcg gcgcggtggc gatcggcgtg tcgggcgcgg ttttggcgat ggcgggggcg
      301 gcgcacagca tgccggcggc gctggaggcg aacaaaaggg cgcggagcgt ggaggcgcgt
      361 gtgtcggtca tcggggaggt ggtccagcgg ctgatgtaag gcgagattgc gatgctttcg
      421 cattgcgatg ggctcgcaat atcaacttcc ggccaaggtt caaccttatt tgcgaataaa
      481 tcgcatttat cgtggcgagt gtgtgcccct cacatgaagt gcccagcaat cgacttacag
      541 aatggcaatc ccctgccgca gcccccacta tgaagcttca gcagaacggg gtggcggcat
      601 ggcggatccg acgggcggga tgggacttgg gcatgccgta gccttgctgg cggccgccgt
      661 cgtcgccgtg cccctgttta agcgcctcgg agccggtgcg gtgctgggct atctggccgc
      721 cggcctggtc atcggaccgt tcggcttcaa gctgatccag gacgccgagg cggttctgca
      781 tgtcgcggaa ttgggcgtgg tgatgttcct gttcatcatc ggactggaga tgcgccctgc
      841 ccggctgtgg agtctgagga aggaaatctt cggtctggga gccctgcagg tgctgacctg
      901 cggcgccctg ctgaccgggg ccgccctggt ggccggcttg tcggtccctg cggccatcat
      961 cgccggcgca ggcttcgccc tgtcttccac cgccatcgtt atgcagctgc tggaagagcg
     1021 gaacgaacag tccgatccgg cgggacaaag ggtcgtttct gtcctgctct tcgaagacct
     1081 gtcgatcgtg ccgctgctgg cgctggtcgc ggtgttcggc ggtatgttcg gcaccgcaat
     1141 cgagaacccc cagccgatct ggctgacggt cgggttcgcc atcgcggctg tcgcctcggt
     1201 ctatgtgatc ggcaaatggg tcatgaaccc ggtgctgagg cttctggccc gctacggcgg
     1261 acgcgagatt atgacggcgg cagcgttgct ggtggtgacg ggcgcggcct gggcgatgga
     1321 tctgggcggg ctgtccatgg ccatgggcgc gtttctggcc ggagtcctgc tgtctgagtc
     1381 caccttccgc caccagctag aggcggatgt cgaaccgttc cgtggcattc tgctcgggct
     1441 gttcttcctc agcgtcggca tgtcgctgga tctcggcgtc gtggtgcgcg actggcagct
     1501 ggtcgttggg ggcatcgccg ccttcatgtt cgccaagatg gccggggtct atgcgatcgc
     1561 gcggatcaag tccgggcatg gcgaggccct gaagcgggcg gccctgatgg gcgaaggcgg
     1621 ggagttcgcg ttcgttctgt acggggctgc gctggctgcg ggcctgttcg acccccgcag
     1681 tgcggccata ctgtcggcga tcgtcatcct gtccatggcc ctgacgccgg tgcggatgct
     1741 gctggcggac cggttcaagc ctgaggacgt gctgtcgatg gaggggatcg acgaggcgca
     1801 cggcctgcgc gaacgggtgc tgatcatcgg cttcgggcgc ttcgcccagg tcgtggccca
     1861 gccgctactg tcacgcgacg tggacgtctc catcgtcgac atcgacgtcg agatgatcca
     1921 ggccgccggc aagttcgggt tcaaggtcta ttacggcgac ggcgcccgac tggatgtgct
     1981 gcgcgcctcg ggggccggaa ccgccgagac ggtgctggt
//
LOCUS       DASVTM010000510         4546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_741614,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000510 DASVTM010000000
VERSION     DASVTM010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741614"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VF633_14045"
     CDS             complement(<1..1006)
                     /locus_tag="VF633_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX8472229.1"
                     /translation="MRAEVAGQGLRWRLWAPVAFGGGCAIYFALKTEPALWPLLVMAS
                     VAALVWLAARARGLPRAVTLPLMLMMCATLGMAAAKVRTEAVAGPMAPTLEEPTTIEG
                     WVIDVDSPGQNGARVVIAPVSVRGLAAEATPIRMRATVKGDPPPPGTPIRLFAILNPP
                     PPPASPGAYDFGRGAYFEGMGGVAFALSPTRPAVLAEAPWGLRLMMKVNSVRFALAQR
                     IVDRLGPRTGGVAAAMVTGHETWIGREDLDAMRDSGLAHILSISGLHMAVVGGFVFFL
                     ARLGVAAWPWLALRVSGKKVAAIAGLIAVWTYLVISGSPPPAERAAITYSIAFIAILL
                     D"
     gene            1086..2519
                     /gene="gltX"
                     /locus_tag="VF633_14050"
     CDS             1086..2519
                     /gene="gltX"
                     /locus_tag="VF633_14050"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX8472230.1"
                     /translation="MTSPASPRDPSSTVVTRFAPSPTGSLHIGGARTALFNYLYAKGR
                     NGKFLIRVEDTDRERSTDEAVKAIFDGLSWLELFADEEPVFQYARADRHREVVQTLID
                     TGHAYRDYTTAEETGRLRDQAKAERRTFESPWRDRVVEGLEAEQPFVVRFRRPLPGRV
                     EVADEVQGEVRWDNNDLDDLVLLRSDGAPTYNLAVVVDDHDMGVTHVIRGDDHLNNAA
                     RQSLIYDALGWTRPVFAHIPLIHGPDGAKLSKRHGAQAVHEYAEMGYLPEAMRNYLAR
                     LGWAHGDDELFSDAQAQEWFDLSGVGKAPARLDFDKLAHVNAHWMRLADDDSLSKQVL
                     DIHLARGHALGEHDEVRLLRAMPFLKDRARTMNELADQSAFVLRQRPLAIFEKAIPLL
                     TGESGERIARLRDRLKLFASWDVFALEAELKIFAEEEAVGFGKIGPAVRAALTGDGVS
                     PDIAKTLAALGREESLGRLDDALQHTK"
     gene            2584..3885
                     /gene="gltA"
                     /locus_tag="VF633_14055"
     CDS             2584..3885
                     /gene="gltA"
                     /locus_tag="VF633_14055"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX8472231.1"
                     /translation="MTEQAKPAGTATLTYGDKSVDLPVLSGSTGPDVIDIRKLYAATD
                     AFTFDPGFTSTAACESAITYIDGDAGVLLHRGYPIGQLASQSNFLEVCHLLLHGELPT
                     AAEYETFEHTVTQHTMLHAQFDRFFEGFRRDAHPMSIMVGTVGALSAFYHDSLDIHDA
                     RQREISAIRLIAKMPTIAARAYKYHIGQPFVSPRNDLSYAENFLRMCFAVPAEDYVID
                     PVLAKAMDRIFTLHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPSHGGANEE
                     ALNMLKEIGTPDKIPDFIQGVKDKKYKLMGFGHRVYKNYDPRAKVMQESAYEVLAATG
                     RENDPLFVVAKELEKVALSDEYFTSRKLFPNVDFYSGITLSAMGFPTSMFTVLFSLAR
                     TVGWIAQWQEMIGDPAQKIGRPRQLYTGPTERDYVPISARG"
     gene            complement(4091..>4546)
                     /locus_tag="VF633_14060"
     CDS             complement(4091..>4546)
                     /locus_tag="VF633_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472232.1"
                     /translation="HVRSISLGLGTGSITWVEKHEGSWWAMFANYDGRGGEVPRDHRH
                     TTLVRFDDQWRRLEAWALPTSILDRIAPMSISGGGWAPDGRLYLSGHDLRELYVVAIP
                     DGGGVLEHVETYGMEAEGQAIDWDESTPGMLYGITRSSREILAMRVPLR"
BASE COUNT          845 a         1655 c         1351 g          695 t
ORIGIN      
        1 ggtccagcag gatggcgatg aaggcgatgg aataggtgat ggctgcgcgc tcggcgggcg
       61 gcggcgagcc ggagatcacc agataggtcc agaccgcgat cagcccggcg atcgccgcga
      121 ccttcttgcc cgagaccctc agcgccagcc aaggccaggc cgcgacgccc agccttgcaa
      181 ggaagaagac gaaaccgccc acgacggcca tgtgcaggcc cgagatcgac aagatgtgcg
      241 ccagccccga atcgcgcatg gcgtcgaggt cctcgcgccc gatccaggtc tcgtgcccgg
      301 tgaccatcgc cgccgccaca ccgccggtgc gtggcccgag ccgatcgacg atccgctggg
      361 ccagtgcgaa ccgcacgctg ttgaccttca tcatcagccg caatccccac ggcgcctcag
      421 ccaacacggc gggccgcgtc ggcgacagcg cgaacgccac cccgcccatg ccctcgaaat
      481 acgcgcctcg cccgaaatcg taagcgcccg gactggcggg aggcggcggc gggttgagaa
      541 tggcgaacag ccggatcggc gtgcccggcg gcggcggatc gcccttgacc gtagcgcgca
      601 tccggatcgg cgtagcctca gcggccaacc cacgcaccga gacaggcgca atcacgaccc
      661 tcgcgccgtt ctgccccggg ctgtcgacgt cgatgaccca gccctcgatg gtggtcggct
      721 cttccagtgt cggcgccatc ggcccggcca ccgcttcggt ccgcaccttg gccgcagcca
      781 tccccaatgt tgcgcacatc atcagcatca gcggcaaggt cacggcgcgc ggcagacctc
      841 tcgcgcgcgc tgcaagccac accaatgccg cgacactcgc cattacaagc agcggccaca
      901 gcgccggctc agtcttcaag gcgaagtaga tcgcgcaccc gccgccgaac gccaccggcg
      961 cccacaaccg ccagcgcagg ccctgccccg cgacctccgc gcgcacccac gaaaccgtgc
     1021 gccgccaaag gctgggcttt gccgcgtcgg aaggtataag ggcggcccgt acggacctcc
     1081 ctgacatgac ctctcccgcc tctccccgcg acccttcctc gacagtcgtc acccgcttcg
     1141 ccccctcgcc gacaggctcg ctgcatatcg gcggcgcgag aacggcgtta ttcaactacc
     1201 tttacgccaa gggacgcaac ggaaagttcc tgatccgggt cgaggacacc gaccgcgagc
     1261 gctccaccga cgaagccgtg aaagcgatct tcgacggcct gagctggctt gagctgttcg
     1321 ccgacgaaga gccggtgttc cagtacgccc gcgccgaccg ccaccgcgag gtcgtccaga
     1381 ccctgatcga caccggccac gcctaccgtg actacacgac cgccgaggaa accggccggc
     1441 tgcgtgacca ggccaaagcc gagcgccgca cgttcgaatc gccgtggcgt gaccgcgttg
     1501 tcgagggcct cgaagccgag cagccgttcg tcgtccgctt ccgccgcccc ctgccgggcc
     1561 gcgtcgaggt cgcggatgaa gtccagggcg aagtcaggtg ggacaacaac gacctcgacg
     1621 atctggtcct gctgcggagt gatggagcgc cgacctacaa cctcgccgtg gtcgtcgatg
     1681 accacgacat gggcgtgacc cacgtcattc ggggcgacga tcacctgaac aacgccgccc
     1741 gccagtcgct gatctatgat gcgctgggct ggacccgtcc ggtattcgcc cacatcccgc
     1801 tgatccacgg cccggacggc gcaaagctgt ccaagcggca tggggcgcag gcagtgcatg
     1861 aatatgccga gatgggctat ttgcccgagg ccatgcgcaa ctatctggcc cgcctcggct
     1921 gggcccacgg cgacgacgaa ctgttctccg acgcacaggc gcaggaatgg ttcgacctgt
     1981 ccggcgtcgg caaggctccg gcccggctcg acttcgacaa gctggcccac gtcaacgccc
     2041 actggatgcg gctggccgac gacgacagcc tgtcgaaaca ggtcctcgac atccaccttg
     2101 cgcgtggcca tgcgctcggt gagcatgacg aggtccggct gctccgcgcc atgcccttcc
     2161 tcaaggaccg cgccaggacg atgaacgaac tggccgacca gagcgctttc gtgctgcgcc
     2221 aacgcccgct cgcgatcttt gaaaaggcca tccccctgct gaccggcgag tccggtgagc
     2281 ggatcgcccg cctccgtgat cggctgaagc tgttcgccag ttgggacgtg ttcgcactcg
     2341 aggccgaact gaagatcttc gccgaggaag aagccgtcgg cttcggcaag atcggccccg
     2401 cggtgcgcgc tgccctgacc ggagacggcg tctcgcccga catcgccaag accctcgcgg
     2461 ctttaggccg tgaagaatcg ctagggcgct tggatgatgc gctgcaacac actaagtgac
     2521 acccacgact aacaaagggc caggcgtccc ccgctgcggg tccaggtttt ccaaggggct
     2581 acaatgactg aacaagccaa acccgccggc acggcgaccc tgacctacgg cgacaaatcc
     2641 gtagacctgc ccgtcctgtc cggttcgact ggccccgacg taatcgacat tcgcaagctt
     2701 tacgcggcga ccgacgcctt caccttcgac cccggcttca cgtccaccgc cgcttgcgaa
     2761 agcgccatca cctacatcga cggcgacgcc ggcgtgctgt tgcaccgcgg ctatccgatc
     2821 ggccaactgg cctcgcaatc gaacttcctc gaagtctgcc atctgctgct gcatggcgaa
     2881 ctgccgacgg ccgccgagta tgagacgttc gagcacaccg tcacccagca cacgatgctg
     2941 cacgcccagt tcgaccgctt cttcgagggc ttccgtcgcg acgcccaccc gatgtcgatc
     3001 atggtcggca ccgtcggcgc cctgtcggcc ttctatcacg acagcctcga catccatgac
     3061 gcgcgccagc gcgaaatctc ggccatccgc ctgatcgcca agatgccgac gatcgccgcc
     3121 cgcgcctata aatatcacat cggccagccg ttcgtttcgc cgcgcaacga cctcagctat
     3181 gccgagaatt tcctgcgcat gtgcttcgcc gtcccggcgg aggactatgt gatcgacccg
     3241 gtactcgcca aggccatgga ccggatcttc accctgcacg ccgaccacga gcagaacgca
     3301 tcgacctcaa ccgtccgtct ggccggttcg tcgggtgcca acccgttcgc ctgtatcgcc
     3361 gccggcatcg cctgcctgtg gggcccgtcg catggcggcg ccaacgaaga ggcgctgaac
     3421 atgctcaagg aaatcggcac gcccgacaag attcctgatt tcattcaggg cgttaaggac
     3481 aagaaataca agctgatggg cttcggccac cgcgtgtaca agaactacga cccgcgcgcg
     3541 aaggtcatgc aggaaagcgc ctacgaggtg ctggccgcca cgggccgtga gaacgatccg
     3601 ttgttcgtcg tcgccaagga actcgaaaag gtcgccctga gcgacgaata cttcacctcg
     3661 cgcaagctgt tcccgaacgt cgacttctat tcgggcatca ccctctcggc gatgggcttc
     3721 ccgacctcga tgttcaccgt gctgttctcg ctggcccgca ccgtcggctg gatcgcccag
     3781 tggcaggaaa tgatcggcga cccggcccag aagatcggcc gtccgcgcca actctacacc
     3841 ggcccgaccg aacgcgacta cgtgccaatc agcgcacgcg gctaagccgt tcgatcagga
     3901 ttgaagacgc aaacgccggg gccaaagccc cggcgttttt cttggcgcca tgcccagacg
     3961 ctttcgaccc gccggaaccc gcaaaagtcc cacggccgcc tttccacccg atcaacctcg
     4021 ctaacgtcca ggccggtggc cagcgtgccc gccgcaacgt ctttcggcct gccagggggc
     4081 aaacgctaga tcagcgcagc ggaacccgca tcgccaggat ttcacggctc gaacgggtga
     4141 tgccgtacag catgcctggc gtgctctcgt cccagtcgat cgcctgcccc tcggcctcca
     4201 tgccgtaggt ctcgacgtgc tccagcaccc cgccgccatc gggaatagcg acgacgtaga
     4261 gctcccgaag gtcgtggccg ctcaggtaca gccggccatc gggcgcccag cccccgcccg
     4321 aaatgctcat cggcgcaatc cggtcgagaa tcgaggtcgg caaggcccag gcctcaagcc
     4381 gccgccactg gtcgtcgaac cgaaccagag tcgtatgccg gtgatcacga ggaacctcgc
     4441 cgccccggcc gtcatagttg gcgaacatag cccaccacga tccctcgtgc ttctcgaccc
     4501 aggtgatgga tcccgtgccg aggccgagcg agatgctgcg gacgtg
//
LOCUS       DASVTM010000511         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_153032,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000511 DASVTM010000000
VERSION     DASVTM010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153032"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VF633_14065"
     CDS             complement(<1..364)
                     /locus_tag="VF633_14065"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX8472233.1"
                     /translation="MGKIFTDATAALAGVTFDGMTVMSGGFGLCGIPENLITGLKASG
                     VKGLTVISNNAGVDGFGLGQLLETHQIAKMISSYVGENKEFERQYLAGELELEFNPQG
                     TLAERIRAGGAGIPAFFTA"
     gene            599..1489
                     /locus_tag="VF633_14070"
     CDS             599..1489
                     /locus_tag="VF633_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX8472234.1"
                     /translation="MTAALLVGVGIGLVLTPDGRAWLRHPTLLLGSSANASAETEPEP
                     AIKAVASVPSVPTGPMITPLKTRMASGKLRVGVFGDSMGDGLWTGLYRDLNATPGVTV
                     TKFSEVSTGLSRYDYVDIQAKTERQITETPIDVAVVMFGTNDAQGISMDGQIHPFGSA
                     GWKAAYGKRVDDLVGLLRSRDIAVYWVGLPKMKRASFDARMTLINEVVAAHMAALNVP
                     YIPTVALTSDGNGDYEPYLTVHDSGRRQMMRANDGIHMSMAGYLRITEPVVERLKADA
                     GLAAAPVTDEASASAGGAGA"
     gene            1782..2327
                     /locus_tag="VF633_14075"
     CDS             1782..2327
                     /locus_tag="VF633_14075"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX8472235.1"
                     /translation="MVGVTLTDLPNHPVPALDASVDLVVLEYGVNEGFDDTLDTAAYE
                     RLLRNEIGRLKAEAPQAALLILGAPEALRSEGGGNCPDDGEARWRSPTMLAVVRDIQQ
                     RVSASTGVAYWDWHGRMGGDCSAFRLARPGPNGEEPMMRGDHVHFTQAGADWIGSLLF
                     ADLMTAGREWYRQRRLYREGR"
     gene            2328..>2935
                     /locus_tag="VF633_14080"
     CDS             2328..>2935
                     /locus_tag="VF633_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEX8472236.1"
                     /translation="MLFPTLAFGVFFLFVYFTSWSLDRENGRRKLFLLLASWFFYAQW
                     DWRFVGLLIGSAVMNWGVAALIARKPVDGEGRRIGAGWLVGLGVAANMLILGFFKYYG
                     FFAVQMGELLIQFGWERDLPLLQVILPVGISFFTFQGISYIVDVKRGKTPVARSLLDV
                     MLLMSFFPHLVAGPIVRASDLLPQFDRMPRLTREMAIHGLLL"
BASE COUNT          466 a          906 c          988 g          575 t
ORIGIN      
        1 tggcggtgaa gaaggcaggg atgcctgcgc cgcctgcgcg gatgcgctcg gccagtgttc
       61 cctgcggatt gaactccagc tccagctcgc cggccaggta ctggcgttca aactccttgt
      121 tctcgccgac gtaggacgag atcatcttgg cgatctgatg cgtctccagc agttggccga
      181 ggccgaagcc gtccacgcca gcgttgttgg agatgaccgt cagtcccttg acgcctgacg
      241 ccttcaggcc ggtgatcagg ttctccggga tcccgcacag gccgaagccg cccgacatca
      301 cggtcatgcc gtcgaaggtc acgcctgcca gcgcggccgt cgcgtcagtg aagatttttc
      361 ccatcggctc cctttttcac tctttgatga atatttcgat gtccttacgg cgcgtcattc
      421 tgcggggcaa gaccatcctt ggatcggcat acactattgc ccgccccgag agcccggagc
      481 cacagttaac ctttttcttc gctcacgagg ggcaggcggt agcgcgggcc cggacatcgg
      541 ctaaggttcc cggtcagtag aacctgcggg tggggtcgtg cgggcgaaca tcaactcctt
      601 gacggccgcg ttgctggtcg gtgtaggcat cgggctggtg ctgacgccgg acggtcgcgc
      661 gtggctgcgc catccgaccc tgttgttggg ctcaagcgca aacgcctctg ccgaaaccga
      721 gccggagcct gccatcaagg cggtggcgag cgtgccgagc gtgccgaccg ggccgatgat
      781 cacgccactc aagacacgga tggcgagcgg gaaactccgc gtgggcgtgt tcggcgactc
      841 catgggcgac ggtctgtgga cgggacttta tcgcgacctg aacgccacgc ctggtgtaac
      901 ggtgaccaaa ttcagtgagg tctccactgg cctgtcacgc tacgactacg tcgatattca
      961 agccaagacc gaacgccaga tcaccgagac gccgatcgac gtcgcagtgg tgatgttcgg
     1021 gaccaatgac gcccagggca tcagcatgga cggccagatt caccccttcg gatcggctgg
     1081 ctggaaagcg gcctacggca agcgggtcga tgatctggtc ggtctgctgc gcagccgcga
     1141 catcgctgtc tactgggtcg gtttgccgaa gatgaagcgc gcctccttcg acgccaggat
     1201 gactctgatc aacgaggtcg tcgcggccca tatggcggcg ttgaacgtgc cctatatccc
     1261 gaccgtcgcc ctgacctcgg acgggaatgg cgactacgaa ccctatctga ccgtccacga
     1321 cagcggccga cgtcagatga tgcgggcgaa cgacggcatc cacatgtcca tggccgggta
     1381 tttgcggatc acggaacccg tggtcgagcg gttgaaggcc gacgcgggtc tggctgcggc
     1441 gcctgtcacg gatgaggctt cggcgagtgc gggcggcgcc ggggcgtgat ccgggccctg
     1501 attctggctt cgatgttggg ggccgtcccg cttgtggcga cagctcagga gcggccgtgg
     1561 acgccgctgc cctcgccgca gagcccgtcc tgtcgcgacg gtttgtgtca ggccgatacg
     1621 ctgtcggctc tgttccgggc gctggacggg cgctggggcg gagtcgtccg catcgtccaa
     1681 ttcggcgaca gccatacagc gggacaactg atttccagcg cacttgaaat gcggctcaag
     1741 gccaggttcc ggcccgcgac atccatatcg agccgatcgg cgtggtcggg gtcaccctga
     1801 cggacctgcc gaaccatccg gtgccggccc tggatgcatc agtggacctc gtcgtgctgg
     1861 aatatggggt caacgaaggc ttcgacgaca cgctggatac ggcagcctac gaacggttgc
     1921 tgcggaacga gatcggacga ttaaaggccg aggccccgca ggcagccttg ctaatcctgg
     1981 gtgcgcccga ggcgctgcga agcgagggcg gcggaaactg tcccgatgat ggcgaagccc
     2041 gctggcgctc gcccaccatg cttgcggtgg tgcgcgacat ccaacagcgc gtctcggcca
     2101 gcacgggggt ggcctactgg gactggcacg ggcggatggg gggcgactgc tccgcctttc
     2161 gcctggctcg gccgggcccc aacggcgaag agccgatgat gcgaggcgac catgtgcatt
     2221 tcacgcaagc cggggccgac tggatcgggt cgttgctctt cgccgatctg atgacggcgg
     2281 gccgcgagtg gtatcgccag cgtcgcttgt atcgggaggg gcgctagatg ctgttcccga
     2341 ccctggcctt tggcgtcttc ttcctgttcg tctatttcac ctcgtggtcg cttgatcgcg
     2401 agaacggtcg ccgcaagctg tttctgctgc tggccagctg gttcttctat gcgcagtggg
     2461 actggcggtt tgtcggtctt ctgatcggct cggccgtgat gaactggggg gtggcggccc
     2521 tgatcgctcg caagcccgtc gacggggagg gacgtcggat cggcgctggc tggctggtcg
     2581 ggctcggcgt cgcggccaac atgctgatcc tcggcttctt caaatattac ggcttcttcg
     2641 ctgtgcagat gggtgagctg ctgatccagt tcggctggga gcgggacctg ccgctgctgc
     2701 aggtgatcct gcccgtcggc atcagcttct tcaccttcca gggcatcagc tacatcgtcg
     2761 acgtcaagcg cggcaagacg ccggtcgcgc ggtcgttgct cgacgtcatg ctgctgatga
     2821 gcttcttccc gcatctggtc gccggaccga tcgtgcgggc gtcggacctg ctgccgcagt
     2881 tcgaccggat gccgcgcctg acgcgcgaga tggctatcca cgggttgttg ctgat
//
LOCUS       DASVTM010000512         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_316411,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000512 DASVTM010000000
VERSION     DASVTM010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316411"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VF633_14085"
     CDS             <1..874
                     /locus_tag="VF633_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8472237.1"
                     /translation="YFYTRFFMERVVKVDASTVDGMILAVTVASAFLYVLFGWLSDKI
                     GRKPVMIGGMIVFLVAVFPGFQALTRAANPALAEAQASAPVTVVADPATCAVQFDPVG
                     TAAFASSCDLAKSVLSNAGVNYANIAAPAGSPAVVRVGGVEIASVEGAGLSTAALKSA
                     RTDVETRVKAALVQAGYPTKADPARFNWWGVFGVLMIFMVAATAVYGPQAAALVELFP
                     TRVRYTAMSLPYNIGTGWIGGLLPAASFALVAWSGNIYFGLWYSVAFTAIAALVALLF
                     LPETKGRDLDTIGD"
     gene            947..2401
                     /locus_tag="VF633_14090"
     CDS             947..2401
                     /locus_tag="VF633_14090"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX8472238.1"
                     /translation="MNTAARDAGLKLLRQHAFIAGEAVPAGPNGIAVDDPATGEIIGH
                     IPDLGVAETERAIQAAHDAFPAWARTDPHKRARFLRDWAALIDENTEGLGALMAIENG
                     KPFEEAKGEVAYANGFIKWFAGQAERIIGETQDSPLGHVILTFREAVGPCALITPWNF
                     PAAMLTRKLGPAFAAGCTAVVKPASQTPFTAIALAELAYEAGLPKGALSIVTGDAATI
                     GGVLTASPLIRKLSFTGSTPIGRLLAEQSAKTLKRVSMELGGAAPLIVFADADLDQAV
                     AETIKGKFRNAGQTCVCPNRVYVEASVAEEYASKLAAEVAKIPVGPAFEDGVKVGPLI
                     EDKALDKVEKHLAQIVADGGKVLTGGARHELGGRFFQPTVTLGGNDELFRREETFGPL
                     IPVFPFETEEEVMEMANDSEFGLASYLFTKDLDRAMRIGRHIEAGMIGINTGLISTAV
                     APFGGVKSSGYGREGSIHGLDEYVDIKQVTLALK"
     gene            complement(2406..>2626)
                     /locus_tag="VF633_14095"
     CDS             complement(2406..>2626)
                     /locus_tag="VF633_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8472239.1"
                     /translation="EPQAVHRGLVVEQSRADLEGPVKTVASPIRMSKTPVAYDAAPPA
                     LGQDTDNVLEAMGLDAARLATLRGKNVI"
BASE COUNT          426 a          892 c          843 g          465 t
ORIGIN      
        1 ctatttctac acgcgcttct tcatggaacg ggtggtgaag gttgatgcat caaccgttga
       61 cggtatgatc ctggcggtca ccgtcgcctc ggccttcctc tatgtcctgt tcggctggtt
      121 gtcggacaag atcggccgga aacccgtgat gatcggcggc atgatcgtgt ttctggtcgc
      181 ggtcttcccc ggcttccagg ccctgacccg cgccgccaat cccgcgctgg cagaggctca
      241 agcctcggcg cccgtcacgg tcgtcgccga tccggccacc tgcgcggtgc agttcgatcc
      301 cgtcggcaca gcggcctttg cctcgtcctg cgatctggcc aagtcggtcc tgtccaacgc
      361 cggagtcaat tacgccaaca tcgccgctcc ggctggatcg cctgcggtcg tgcgcgtcgg
      421 cggcgtcgag atcgcttcgg tcgagggcgc aggcctgtcg actgcggctc tcaaatcagc
      481 gcgaaccgac gtcgagaccc gggtcaaggc ggcgctggtt caggccggtt atccgaccaa
      541 ggccgatccc gcgcgcttca actggtgggg cgtgttcggg gtgctgatga tcttcatggt
      601 cgccgccacc gccgtctatg gtccgcaggc cgccgcgctg gtcgaactgt tcccgacgcg
      661 ggtgcggtac acggccatga gcctgcccta taacatcggc accggctgga ttgggggcct
      721 gttgcccgcc gccagtttcg cgctcgtggc ctggtcggga aatatctatt tcggcctctg
      781 gtactcggtc gcctttaccg ccatcgccgc cctcgtggcc ctgctgttcc tgccggagac
      841 caaggggcgc gacctcgaca ccatcggaga ctaaccggcg gtttttatgc gccctgacgc
      901 gacgtcactg cgttaaaggt cgatcgccac tacgaagggt cacgccatga acaccgccgc
      961 ccgcgacgcc ggtctgaagc tcctgcgcca acacgccttc atcgccgggg aggccgttcc
     1021 cgccggtccg aacggcatcg ccgtcgacga ccccgcgacc ggcgagatca tcggccacat
     1081 ccccgatttg ggcgtggccg agaccgagcg cgccattcag gccgcccacg acgccttccc
     1141 cgcatgggcg cgcaccgatc cgcacaagcg ggcgaggttc ctgcgcgact gggccgcgct
     1201 gatcgatgaa aacaccgaag gtctcggcgc cctgatggca atcgaaaacg gcaagccgtt
     1261 cgaggaggcc aagggcgagg tcgcctatgc caatggcttc atcaaatggt ttgcgggaca
     1321 ggccgagcgg atcatcggcg agacccagga cagccccttg ggccacgtca tcctgacgtt
     1381 ccgcgaggcc gtcggcccct gcgccctcat cacgccgtgg aatttcccgg cggccatgct
     1441 gacgcggaaa ctcggccccg ccttcgccgc cggctgcacc gccgtggtca agccggccag
     1501 ccagactccc ttcaccgcca ttgcgcttgc ggagctggcc tatgaggcgg gcctgcccaa
     1561 gggcgcgctc agcatcgtca ccggtgacgc ggcgaccatc ggcggcgtgc tgaccgcgtc
     1621 accgctgatc cgcaaactgt ccttcaccgg ctcgaccccg atcgggcggt tgctggccga
     1681 acagtcggcg aaaacgctca agcgcgtgtc gatggaattg ggcggcgctg cgcctcttat
     1741 cgtgttcgcc gacgccgatc tggatcaggc cgtggccgag accatcaagg gcaagttccg
     1801 gaacgcaggc cagacctgcg tctgtcccaa ccgggtctat gtcgaagctt cggtcgcgga
     1861 ggaatatgcg tcgaaactgg ccgccgaagt ggcgaagatc cccgtcggac cggcgttcga
     1921 ggacggcgtg aaggtcggcc ccctgatcga ggacaaggcc ctcgacaagg tcgagaaaca
     1981 cctcgcgcag atcgtcgccg acggcggcaa ggtgctgacc ggcggggctc gtcatgaact
     2041 cggcggccgc ttcttccagc cgacggtgac gctgggcggc aacgacgaac tgttccggcg
     2101 cgaggagacg ttcggcccgc tgatcccggt cttcccgttc gagaccgagg aggaggtcat
     2161 ggagatggcc aacgactcgg agttcggcct cgcatcctat ctgttcacga aggatctgga
     2221 ccgggccatg cgcatcggcc ggcatatcga ggcggggatg atcggcatca acaccggcct
     2281 gatctccacc gccgtcgcgc cctttggcgg ggtcaagtcc tccggctatg gccgcgaggg
     2341 ctccatccac ggtctcgacg aatatgtgga catcaaacag gttacgctgg ccctcaaata
     2401 acgggtcaga tcacgttctt gccgcgcagg gtggcaagcc gcgccgcatc cagtcccatg
     2461 gcctcgagca cattgtccgt gtcttgaccc agcgctggcg gggcggcgtc ataagccact
     2521 ggggtcttcg acatccggat gggcgaggcg acggttttca ccgggccctc gagatcggcg
     2581 cgggactgct cgacgaccag tccccggtga acagcctgag gctcct
//
LOCUS       DASVTM010000513         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_848110,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000513 DASVTM010000000
VERSION     DASVTM010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848110"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VF633_14100"
     CDS             complement(<1..1253)
                     /locus_tag="VF633_14100"
                     /inference="COORDINATES: protein motif:HMM:NF037279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcbI family polysaccharide biosynthesis putative
                     acetyltransferase"
                     /protein_id="HEX8472240.1"
                     /translation="MRVYVHGNCQAPALADLIGQACGGAMDVVSRQVYSIDLEAEGAA
                     HIHDVETADVILTQPVADGYRGTDILSTSWMRARARKDAIFLVFPVIYHRGLQPQCYT
                     LGDFHAGRLAYHDAHALDYFLMGEDADSFVRATNRSDFLPESFVLSELSRTTLELLRR
                     ERALAVDLPVSDIIATRLSSGQPMYTVNHPSRAVMVDLADRVLERLGRPERASPDGID
                     MLDLFIMPPYLSTALALGHSGSGIWLDDMSADGVMESRLDFFRHVFQSYQEIGRDRLR
                     RAVAENPEITGYLARYEHARMQGASRDMSDLIDALYETFLDRPAAPDEILHHLQTVRL
                     SGFSMMVSAFAGSPEFHARGGAASMMNRFPKTHAMPVRRPHVRIPHPGQLVPTVASDR
                     LASDPQSLKARLQRIWRAVINSPPEP"
     gene            complement(1354..2550)
                     /locus_tag="VF633_14105"
     CDS             complement(1354..2550)
                     /locus_tag="VF633_14105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8472241.1"
                     /translation="MKTALLIVERDFLVTHVGVRQVVLFYWRLLESAGYRVRLATPDS
                     GCLITATPVSLAEVMIALNRGRSDAPDWRSTDKPDAWERAVRDGRPKDIVKWTDDKIS
                     TRDVSLSIVTNPWLCANGFPEERFTAGIVYDMVPNLLASGALRLGTVTDIYRFAHAHD
                     VGYRIYQRQADKILSISESTRRDFLAFYPSGDMAARTEVVIPFDPPFSPRILAGPALP
                     QQRRKLLLVNVLDPRKNFSGVERAVKLAMRETSFDIDVVGRERMPGAAAQAFLTNLAD
                     AGAHVRWYRSASAPCLQRLYAEADVLLFPSLYEGLGLPILEAQAHGTPVVSSNTSSCG
                     EINLNPGLTFAPSDIEGLAVAMLEVFAGTSPAVAGPALAAAQTTYLKRRSALPSDWLG
                     ERSATG"
     gene            complement(2547..>2989)
                     /locus_tag="VF633_14110"
     CDS             complement(2547..>2989)
                     /locus_tag="VF633_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472242.1"
                     /translation="LQSTAFVRMGEVLAFEGSDGILDAAKGGFIFGKGWAAVPDAVIW
                     KAAGSPGISLRFQGAHTDIALMLQFSDAETAARLLSVGVNGIREAVLERDAAVVRVLI
                     PQDVVTDSKILHIWMTIDGGPLSLGAMQLTGVAVPPSRSARRPL"
BASE COUNT          587 a          921 c          960 g          521 t
ORIGIN      
        1 ggctccggag gagagtttat gactgcacgc cagatccgct gcagtctggc tttcaggctt
       61 tgcgggtcgc tggccagcct atcggaggcg acagtcggca ccagttgccc cggatgcgga
      121 atgcgcacgt gcggcctccg cacaggcatg gcgtgggtct tggggaaccg gttcatcatc
      181 gatgcggcgc cgcctctggc atggaactcc ggagagcccg caaaggcgga caccatcatc
      241 gagaagcctg aaagcctgac cgtctgcaga tgatggagga tctcgtccgg cgcggcggga
      301 cgatcgagga aggtctcgta aagagcgtcg atcaggtcgc tcatgtccct tgacgccccc
      361 tgcatccggg cgtgctcgta gcgcgcaaga taaccggtta tttctggatt ttcggcgacg
      421 gcccgacgaa gccggtcgcg accgatttct tgatacgact ggaagacatg ccggaagaaa
      481 tccagtcggc tttccatcac gccgtccgcg gacatgtcgt ccagccagat accggatcct
      541 gaatggccga gcgcgagggc ggtcgacaga taaggaggca taatgaacag gtcgagcatg
      601 tcgatcccgt cgggggacgc gcgctcggga cgtccgaggc gttccagcac tcgatccgca
      661 aggtccacca tgaccgcccg tgatgggtgg ttgaccgtgt acatgggttg gccggacgaa
      721 aggcgcgtgg cgatgatatc cgaaaccggc agatcgaccg ccaaggcccg ttcgcgtcgg
      781 agcagttcga gcgttgtgcg cgacaactcg gacagcacga aactctccgg gaggaaatcg
      841 cttcggtttg tcgccctgac gaaggagtcg gcgtcctccc ccatgaggaa ataatcgagc
      901 gcatgggcgt cgtgataggc cagccgcccg gcgtgaaaat cgccgagcgt gtagcattgc
      961 ggctgaagtc cgcgatggta gatcaccggg aaaaccagga agatggcgtc cttgcgggcg
     1021 cgcgcgcgca tccacgacgt cgaaaggatg tctgtgccgc gatagccgtc tgcgaccggc
     1081 tgggtcagaa taacgtcagc agtctccacg tcatggatat gagcggcgcc ctccgcctca
     1141 aggtctatgc tgtaaacctg ccggctgacg acatccatgg cgccgccgca cgcttgtcct
     1201 atgaggtcgg cgagagcggg ggcctggcaa tttccatgga cgtagaccct caactagcag
     1261 gatcctgaaa cgcgattgct cgcagacggt cccacccttt ccttcgcgac gagggccttg
     1321 tcaaccactc gcagccaccc ggtcaggctt cgatcatcca gtggcggacc gctctcccag
     1381 ccagtcggac gggagtgcgc tccggcgttt gagataggtg gtctgggccg ccgccaaggc
     1441 cggtccggcg accgccgggc tagtcccggc gaatacctcc agcatggcga cggccaaccc
     1501 ctcgatatcg gaaggcgcaa acgtcaggcc gggattgagg ttgatctcgc cgcaagacga
     1561 ggtgttcgac gacaccaccg gggtcccatg cgcctgggcc tcgagaatgg ggagacccag
     1621 gccttcatac agagatggaa acagcaaaac gtcggcctcc gcgtaaaggc gctgcaggca
     1681 gggggccgat gccgatctgt accagcgaac atgcgccccg gcgtcagcga ggttggtcag
     1741 gaaggcttgc gccgcagctc ctggcatgcg ctcgcgtcca acgacgtcga tgtcgaacga
     1801 cgtctctcgc atcgccagct tcacggcccg ctcgactccg gagaagttct ttcgcgggtc
     1861 cagcacattg accagaagta gcttgcgtcg ctgctggggc agggctggcc cggccaggat
     1921 cctcggcgaa aacggcggat cgaaggggat gaccacctcg gttctcgcag ccatatcgcc
     1981 ggagggataa aaggccagga agtcccgcct tgtgctctcg ctgatcgaca ggatcttatc
     2041 cgcctgccgc tgatagatgc gatagccgac gtcgtgcgcg tgagcgaagc ggtagatgtc
     2101 cgtcaccgtc ccaagtcgga gggccccgct ggccagcagg ttgggaacca tgtcgtagac
     2161 aatgcctgcc gtgaatcgct cttcgggaaa cccgttcgcg catagccatg gattggtgac
     2221 gatcgaaagc gagacgtctc gcgtgctgat tttgtcgtcg gtccatttga cgatgtcctt
     2281 gggacggccg tctcgcacgg cgcgctccca ggcatcaggc ttgtcggtcg agcgccagtc
     2341 gggggcgtcg gatcgccccc ggttcagggc gatcataacc tcggcgaggg agacgggcgt
     2401 cgccgttatc aggcagccac tatccggcgt ggcaagacgt acgcgatacc ctgcgctctc
     2461 cagcagtcgc cagtagaaca gaacgacctg tctgaccccg acatgggtga cgaggaaatc
     2521 acgttcgacg atcaagaggg cggttttcat agcggtcgtc tcgcggaccg ggagggcgga
     2581 acggcaacgc cggtcagttg catcgcgccc agtgaaaggg gaccaccgtc aatcgtcatc
     2641 cagatgtgca ggatcttgct atcggtgact acgtcttgcg ggatcaagac ccttacgacg
     2701 gcggcgtccc gctcaagaac cgcctcccgg attccgttaa ccccgacgga cagcagccgc
     2761 gccgcagtct ccgcatcgct gaactgcagc atcagagcta tatcggtgtg ggcgccctgg
     2821 aacctgagac tgattccggg cgaccctgcg gccttccaga tcacggcgtc cggcacggcg
     2881 gcccagccct tgccaaaaat gaacccgcct ttggcggcgt ccagaatgcc atccgaacct
     2941 tcgaaggcca gtacctcccc catacgaaca aaagcggtgg actgcaggg
//
LOCUS       DASVTM010000514         4827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_263976,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000514 DASVTM010000000
VERSION     DASVTM010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263976"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VF633_14115"
     CDS             <1..515
                     /locus_tag="VF633_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX8472243.1"
                     /translation="GAGDSFLNRFAQMATVAPALPLIGGGETKFQPVYVGDVAEAIAR
                     ATTRIDAAGRTFELGGPAVWSFGDILKYILRETGRERALAPLPFFAARIVGGLAQLST
                     MIGIAPVLTKDQVILLETDNVVAPGAEGLDALGIEPTGLEAIAPTYLWRYRKGGQFAK
                     GPAPETLVAA"
     gene            complement(547..1356)
                     /locus_tag="VF633_14120"
     CDS             complement(547..1356)
                     /locus_tag="VF633_14120"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX8472244.1"
                     /translation="MTDWLAAIILGLVEGLTEFLPVSSTGHLLLTQHLLGLTDLRWNA
                     FVVLIQLGAILAVVALYFQRLWSVLIRLPTDPSARRFAISVAIAFVPSAIAGLFLHDF
                     ITTVLFEDLRTICWALIIGGVILLVLERWAPKPHTGDAMALSWRQAFLIGLFQCLALV
                     PGVSRSGATISGGLVLGLEKPAAAEFSFFLAIPTMVAAFGLDLLKTKDSIDLHFAWII
                     AIGFVVSFISGLIVIRWMLAFVSKHGFAPFAWWRIVIGAVGLVLLTTGAVV"
     gene            complement(1392..2570)
                     /locus_tag="VF633_14125"
     CDS             complement(1392..2570)
                     /locus_tag="VF633_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX8472245.1"
                     /translation="MEPAGKPNGRWIGRSEALERLGVKVQTLYAYVSRGRISARPDPD
                     NPRRSQYAAEDITRLSTPTIPGETPAPFVPFEQAPARGEALIRSSLSLVSEGRLFYRG
                     KDAIDLSKTATVEDAARLLWDARDANLLAGLGPRLDGAGGVSPRTRMFASLARRAEED
                     PSSAGKTPEQVKAQAASVLNEVIDAVAGPGPRLFFHQRLGRGWKVLERDSPIIRQALI
                     LIMDHGINPAVIATRAAAGGGASPAGAALAGLATFTASPQAFELAKTIAYVIEARREP
                     RGAAERRMADDGRVPGFGDAAWPEGDPRAEVLIATAQLPADLIAIAREGEAASGVKPN
                     IGLALALVARRLDLPRDGASDMLLIGRLVGLLGHALDQTTDGSPIAARLRYVGPRPGA
                     "
     gene            2704..3750
                     /locus_tag="VF633_14130"
     CDS             2704..3750
                     /locus_tag="VF633_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8472246.1"
                     /translation="MTIPEGSEPTTPPDSGPNPGPAPSAASGADQAAITRRITSISVG
                     VAVLLIALKAFALGASGSVSILASLTDSALDLVASLATFYAVRWAAAPPDAEHHYGHG
                     KGEAMAALVQAGLIFASAVFIGWEATQRMFDPRPVGAGGWAVGVMGISIGLTAGLVWL
                     QTRALKQTGSLAVAGDRSHYAADLAANVVVLIGVVSGTWLRAPGLDAAAGLVVAIWLF
                     WGAFALLKQAADHLLDRSATDADQVAVTTAVLADPRIAGVHALRTRMAGSTVMVQMHV
                     DLDPALTLEAAHAIVEQAEARIRAALPDSDVLIHPDPAGIRKPEPVGTAATVSGAVEL
                     TSATGETPRTGPWS"
     gene            3815..4495
                     /locus_tag="VF633_14135"
     CDS             3815..4495
                     /locus_tag="VF633_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8472247.1"
                     /translation="MTSVLFHFPFDPGSRSARLALGEAKIVVEETAVRPWEPDCPVRD
                     LNPSGMPPVLQTSQNGKPLTLCEANAIMGWVEDQQKSPLLMPTDPAERAEVRRLTTWF
                     DRRFTDEVNAVLLHERMEKPLLRLGPPEAQGLREGRAALREHLVMLEALLGERDWLAG
                     RRLSQADLVAAAHISVLDYFGEIAWKSWPLLKTWYMRLKSRPCFRPLLADKFQGLAPA
                     PHYADLDF"
     gene            4538..>4827
                     /locus_tag="VF633_14140"
     CDS             4538..>4827
                     /locus_tag="VF633_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HEX8472248.1"
                     /translation="MVDPRDIIRNQASALGFDVCRFASASEPWSNGEKLAHFVEAGRH
                     GEMGWMETTLERRAHPTAMWDGARTAIVLGMNYGPEADPLPQMADRSAGYISV"
BASE COUNT          791 a         1676 c         1600 g          760 t
ORIGIN      
        1 ttggcgccgg ggacagcttc ctgaaccgct tcgctcaaat ggcgaccgtg gcccccgccc
       61 tgcccctgat cgggggcggt gagacgaaat tccagcccgt ctatgtcggc gacgtcgctg
      121 aggccatcgc gcgcgccacg acccgcatcg acgccgccgg gcgcaccttc gagctgggcg
      181 gccccgcggt atggagcttc ggcgacatcc tgaaatacat cctgcgcgaa accggccgcg
      241 agcgggcgct ggctcctctg cccttcttcg ccgcgaggat cgtcggcggc ctggcccagc
      301 tgagcaccat gatcggcatc gcgccggtgc tgaccaaaga tcaggtcatt ctgctggaga
      361 ccgacaacgt cgtcgctccg ggagccgagg gtctggacgc actgggcata gagccgaccg
      421 gccttgaggc catcgccccc acctatctgt ggcgctaccg caagggcggc cagttcgcca
      481 aaggtcccgc gccagagaca ctggtcgcgg cctgaccctt tgtccttctc ccgctagaga
      541 agaccctcag actaccgctc ccgtcgtcag cagcacgaga ccgacggcgc cgatgacgat
      601 ccgccaccat gcgaacgggg cgaagccgtg ctttgacacg aaggcgagca tccagcggat
      661 cacaatcagg ccgctgatga acgacaccac gaagccgatg gcgatgatcc aggcgaagtg
      721 aagatcgatc gagtccttgg tcttcagcag gtccagacca aaggccgcga ccatggtcgg
      781 gatggcgagg aagaacgaga actcggccgc tgccggcttc tcgagcccca gcaccagacc
      841 gccggagatg gtcgcgcccg agcgggacac gccgggcacc agcgccagac actggaacag
      901 tccgatcagg aaggcctgac gccagctgag cgccatggcg tcgccggtgt gcggcttggg
      961 cgcccagcgc tcgagcacaa gcaggatgac cccgccgatg atcagcgccc agcagatggt
     1021 gcgcaggtcc tcgaacagga ccgtggtgat gaagtcatgc aggaacagtc cggcgatggc
     1081 tgaaggcacg aaggcgatgg cgacgctgat cgcgaaccgc cgcgccgacg gatcggtggg
     1141 caggcgtatg agcacgctcc acagccgctg gaaatacaac gccacgacgg ccaggatcgc
     1201 gcccagctgg atcagcacca cgaaggcgtt ccagcgcagg tcggtgaggc cgagcaggtg
     1261 ctgggtcagc agcaggtgcc cggtcgagga caccggcagg aactcggtga gcccctccac
     1321 caagccaagg atgatcgccg ccagccagtc cgtcatgaat atcccctgta aggtcccggt
     1381 gggaggcctg atcaggcgcc gggtctcgga cccacatagc gcagacgcgc cgcgataggc
     1441 gagccgtcag tcgtctgatc cagcgcatgg ccgagcagac cgaccagacg cccgatcaac
     1501 agcatgtccg atgcgccgtc gcgaggcagg tccagccggc gcgcgacaag ggccagcgcc
     1561 agaccgatgt tgggcttcac gcctgaagcg gcctcgcctt cgcgcgctat ggcgatcaga
     1621 tcggcgggga gctgggctgt tgcgatcaag acctcggcgc gcggatcgcc ctccggccag
     1681 gctgcgtcgc cgaagccggg cacacggcca tcgtcggcca tgcgccgttc cgccgcaccc
     1741 ctgggctcgc gccgggcctc gatgacatag gcgatggttt tggccagttc gaaagcttgc
     1801 ggcgaggcgg tgaaggtggc gagccccgcc aaagccgccc cagccggtga cgccccgccg
     1861 cccgcggccg cccgcgtggc gatcaccgcc gggttgatgc cgtggtccat gatcaggatc
     1921 agcgcctgac ggatgatcgg actgtcgcgc tccagcacct tccagccacg tccgagccgc
     1981 tgatggaaga acaggcgcgg gccgggaccg gcgacggcgt cgatcacctc gttcagcacc
     2041 gaggcagcct gcgccttgac ctgctccgga gtcttgcctg cactcgaggg gtcttcctcg
     2101 gcgcggcggg ccagggatgc gaacatccgg gtccggggcg agacgcctcc ggctccgtcc
     2161 agccggggtc cgagaccggc tagcaggttg gcgtcgcgcg cgtcccagag cagccgggcc
     2221 gcgtcctcga ccgtcgcggt cttcgacagg tcgatggcgt ccttgccgcg atagaacagc
     2281 cgcccctcgg acacgagact gagcgaggac cggatcaggg cctctccgcg cgccggggcc
     2341 tgttcaaacg gcacgaacgg agcgggcgtc tcgcctggga tcgtcggtgt gctcagccgg
     2401 gtgatatcct ctgcggcata ttggctgcgg cggggattgt ccgggtccgg acgggcggag
     2461 attcgtcccc ggctgacata ggcgtacagg gtctggacct tgacgccgag ccgctcgaga
     2521 gcctcgctcc ggccgatcca gcgaccgttc ggcttccctg caggttccat gcgtacaacg
     2581 tcctgccccg agggaatgca atgcttacca tggggcgttt ccgggaccgg atcaatatgg
     2641 ccaagcttgc cgaaacgtgg gcccgctctg gatttgggca ccgctttcgg cgacaagacg
     2701 accatgacga tccccgaagg ttccgagccg acaaccccac cggactccgg ccccaacccc
     2761 ggcccggcgc ccagcgccgc gtccggcgcc gaccaggcgg cgatcacccg ccggatcacc
     2821 agcatctcgg tcggtgtcgc cgtccttctg atcgcgctca aagccttcgc cttgggagct
     2881 tccggctccg tctcgatcct cgcctcgctc acggactcgg cgctcgatct cgtcgcttct
     2941 ctggccacct tctacgccgt gcgctgggcc gcagcgccgc ccgatgctga gcaccactat
     3001 ggtcacggca agggtgaggc catggccgct ctggtgcagg ccgggctgat cttcgcctcc
     3061 gccgtcttca tcggctggga ggcgacccag cggatgttcg atccgcgtcc cgtcggcgca
     3121 ggcggctggg ccgtgggcgt catgggcatc tccatcggcc tgaccgccgg actggtctgg
     3181 ctacagaccc gggcgctgaa acagaccggc tcgctggccg tcgccggaga ccgcagtcac
     3241 tacgccgccg atctcgcggc caatgtggtg gttctgatcg gcgtggtctc cgggacatgg
     3301 ttgcgcgcgc ccggactgga tgccgcggcg ggtctggtcg tggcgatctg gctgttctgg
     3361 ggagccttcg ccctgctcaa acaagccgcc gaccacctgc tcgaccgatc ggcgaccgat
     3421 gccgatcagg tcgcggtgac caccgccgta ctggccgatc ctcgcatcgc cggcgttcac
     3481 gccctgcgca cgcggatggc cgggtcgacc gtcatggttc agatgcacgt cgatctcgac
     3541 cccgccctga cgctggaagc cgcccacgcc atcgtcgagc aggccgaggc ccgcatccgc
     3601 gccgccctgc cggacagtga tgtcctgatc caccccgacc cagcgggaat acgcaagcca
     3661 gagccggtcg gcactgccgc gacggtttcg ggcgcggtcg agctgaccag cgcgacaggg
     3721 gaaacccctc gcaccggtcc ttggtcgtaa gatttgggct gaacccggtc gcggtaaacg
     3781 cggtcttaag aggggctgcg tcatggatgc gcgcatgacg tccgtgctgt tccatttccc
     3841 gttcgacccc ggctcccgct cggcgcggct ggccttgggc gaggcgaaga tcgtggtcga
     3901 ggagaccgct gttcgcccgt gggagcccga ttgtccggtg cgcgacctca atccctccgg
     3961 catgccgccc gtgttacaga cctcgcagaa cggcaagccc ctgaccctgt gcgaggccaa
     4021 cgccatcatg ggttgggtcg aggatcaaca aaagtcgccg ctgctgatgc ccaccgatcc
     4081 ggccgaacgc gccgaggtcc gccgcctgac cacatggttc gaccggcgct tcaccgacga
     4141 ggtcaacgcc gtcctgctgc acgaaaggat ggagaagccc ctgctgcgtc tcggtccgcc
     4201 cgaggcccag ggcctgcgag aaggccgcgc ggcgctgcgc gagcatctgg tcatgctgga
     4261 agccttgctc ggcgaacgcg actggctggc tggtcgccgt ctcagtcagg ccgatctggt
     4321 tgccgccgct catatctcgg tgctggatta ctttggagag atcgcctgga aatcctggcc
     4381 cctgctaaag acctggtaca tgcggctgaa gtcgcgtccc tgcttccgcc cgttgctggc
     4441 tgacaagttc cagggtctgg ccccggcccc ccattacgcc gacctcgact tctaggcgcc
     4501 tttgatcgcc gcccggtctg tgagacagca gcccgcaatg gtcgatcctc gcgacatcat
     4561 ccgaaatcag gcgtccgcgt tgggcttcga cgtctgtcgc ttcgcctcgg ccagcgagcc
     4621 gtggagcaat ggcgagaagc tggcgcattt cgtcgaggcg gggcggcacg gcgagatggg
     4681 ctggatggag acgacgctgg agcgccgcgc ccacccaacc gccatgtggg acggcgcgcg
     4741 aaccgccatc gtcctcggca tgaactatgg tcccgaggcc gatcccctgc cccagatggc
     4801 cgaccgctcg gcgggctata tctctgt
//
LOCUS       DASVTM010000515         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_153567,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000515 DASVTM010000000
VERSION     DASVTM010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153567"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..308)
                     /locus_tag="VF633_14145"
     CDS             complement(126..308)
                     /locus_tag="VF633_14145"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX8472249.1"
                     /translation="MSDDKSKLGDGDRRRISLSEEYEVRDWAEKFGVSQDELRASVRR
                     VGNMADDVERDLRGGN"
     gene            complement(301..492)
                     /locus_tag="VF633_14150"
     CDS             complement(301..492)
                     /locus_tag="VF633_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472250.1"
                     /translation="MSKSSPTRQGPLEPEARKNAVVVEDPSRDAIVMTADEADLSGIR
                     MLEEAAKARENESNGERDE"
     gene            632..1711
                     /gene="dinB"
                     /locus_tag="VF633_14155"
     CDS             632..1711
                     /gene="dinB"
                     /locus_tag="VF633_14155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX8472251.1"
                     /translation="MNNRLVRKIIHVDMDAFFASVEQRDNPALRGRPVAVGYAAQRGV
                     VAAASYEARVFGVRSAMPSTTALRQCPDLVFVPPRFEVYRAVSKQIQAIFAEYTPLVE
                     PLSLDEAYLDVTHNLRGLPTASETAVEIRARILEQTGLTASAGISYNKFLAKLASDQR
                     KPDGQFVVPPGRGEAFVETLPVKRFHGVGPVTAAKMARLGIETGEDLRRQSLAFLQQH
                     FGKSGPWYYAISRGEDDRAVNPNRERKSSGSETTFGSDLTDPSAIEAGVLAMAGDVWN
                     WCEKSNAYGRTVTVKVKWANFQQSTRSRSFPEPVTSEARLEEVSLLLVRSLYPPARGI
                     RLVGVSLSNFTAPAPSASDQLALAS"
     gene            1771..1867
                     /locus_tag="VF633_14160"
                     /pseudo
     CDS             1771..1867
                     /locus_tag="VF633_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078699.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          306 a          617 c          585 g          359 t
ORIGIN      
        1 ctttgcggcg gtgtcgaacg atggtggtgg ggccggctcc tgccaaaggt tgatcggcca
       61 gatgatcgca gccgggtcgt tccgcgcctg agactcgcgg cccgtgacag cgtagtcgat
      121 cctgcttagt tgcccccgcg gagatcccgc tccacgtcat cggccatgtt gccgacgcgg
      181 cggaccgagg cccgcaactc atcttgggaa accccgaact tctcggccca gtcgcgaact
      241 tcgtattcct cgctgagact gatgcgtcga cggtcgccgt cgccgagctt ggatttgtcg
      301 tcactcatca cgctctccat tagattcgtt ttcacgcgcc ttggcggctt cctcaagcat
      361 gcgaatgccc gagaggtcgg cttcgtcggc ggtcatcaca atcgcgtccc gcgacggatc
      421 ctcgaccacg acggcgttct tgcgagcctc gggctctaga ggcccttggc gggtggggct
      481 ggacttgctc ataggcggag aatgctccgc tttctcgtga agttcccggc gaatggccgg
      541 tcgcgaaaac gccgaatcct ttaaatcgga ctgcgagtgc cgagcgcgct tgccgttcac
      601 tatttgttcc agatttgcta tatcgcctgg aatgaacaac aggctcgtgc gcaagattat
      661 tcacgtcgac atggacgcct tcttcgcgtc ggtcgaacag cgcgacaacc cggccctgcg
      721 cggccgcccg gtggccgtcg gatacgcggc ccagcgcggc gtcgtggcgg cggccagtta
      781 tgaggcgcgt gtcttcgggg ttcggtcggc catgccctcg acgacggcct tgcggcagtg
      841 ccccgacctc gtcttcgtgc cgccccggtt tgaggtctat cgcgctgtct cgaaacagat
      901 ccaggcgatc tttgcggagt acacccctct ggtggagccc ctgtccctcg acgaggccta
      961 tctcgacgtc acccacaact tgcgaggcct tccgactgca tcggagaccg ccgtcgagat
     1021 ccgcgcgcgc atcctggagc agaccggcct gaccgcgtcg gccgggatct cctacaacaa
     1081 gtttctggcc aaactcgcct ctgatcagcg caagccggac ggacagttcg tcgtcccgcc
     1141 cgggcgcggc gaggccttcg tcgagaccct gccggtgaag cgcttccacg gggtcgggcc
     1201 ggtgacggcc gccaagatgg cccggctggg aatcgagact ggcgaggatt tgcggcgcca
     1261 gtcactggcc tttctgcagc agcatttcgg caagtccggt ccctggtact acgccatctc
     1321 gcgcggcgag gacgatcgcg ctgtgaatcc gaaccgcgag cgcaagtcat cgggatcaga
     1381 gacgaccttc ggcagcgacc tgactgatcc gtcggcgatc gaggcgggcg tcctcgccat
     1441 ggccggcgac gtctggaact ggtgcgagaa gtcgaacgcc tacggacgca ccgtgacggt
     1501 gaaggtcaaa tgggccaact tccagcaatc gacgcgcagc cggtcctttc ccgagccggt
     1561 gacctccgag gcgcggctcg aagaggtcag cctcctgctg gttaggtccc tctatccgcc
     1621 cgccaggggc attcgtctgg tcggcgtcag cctgtccaac ttcacggcgc cggcgccctc
     1681 ggcgtcagac cagctcgccc tggcttcatg agcgtgcacc ttccgaacgc tggcgtgatt
     1741 gacgcctgga acggaacgga catgcatgtc accattgccc tgggccggct ttagggggcg
     1801 tcgaactacg ccttcatctg gaacatgggc ggcgagaacc tcgactacac cgacatgaac
     1861 aagctcg
//
LOCUS       DASVTM010000516         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_316628,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000516 DASVTM010000000
VERSION     DASVTM010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316628"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VF633_14165"
     CDS             complement(<1..1079)
                     /locus_tag="VF633_14165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8472252.1"
                     /translation="MAGSLQVEIIKPEALSPAEETLWRAMLAANPDLASPYFRPEFTR
                     IAGSISPEAAVAVFRQDGEIVGFFPHQTRGGTIQPLGAPMNDYHGVIAFPGRAPDLDT
                     VASLLNVPRLRVGAWVGEAHEAEDRETLMVAMPEGPDGDSYGAWYAERRQTCGKYFKD
                     KERARRSLETELGAITVERDLRDPALLDQLIVLKRDQYRRTGRHDIFDCGWTADLLHA
                     LLQARGEDDFGASMAAMYAGGKLVAVEYSLHAGGRYHFWFPVYEPTLARCSPGILLSL
                     DTMRLASARGYRVFDFGFGGEGYKKYFCNAHQTVREAVIVRPSLSAGLSNAAVGLLNK
                     AGKMRGDRLRTSVRRRWAAIEACETT"
     gene            1239..1421
                     /locus_tag="VF633_14170"
     CDS             1239..1421
                     /locus_tag="VF633_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472253.1"
                     /translation="MTDANTPKDRPKNRTLENENQRNPSSDGEAPRPATEPPGSEGSS
                     NSGKTATDPATGEENG"
     gene            1418..2242
                     /locus_tag="VF633_14175"
     CDS             1418..2242
                     /locus_tag="VF633_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8472254.1"
                     /translation="MRLTTVLLTAAASVGLGLLISGVARAQTAEPGTSLLASEPVIPA
                     PTEAPATDAPSCPVSSDSLVLAGDGLKRSKAAVALNRLNILAVGSSSIEGVGASKAEL
                     GFVPLLQTDLRNRLPDVAVNVWNRGIGGETAHETVNRLEAEIQRFRPDLVIWQLGTND
                     VLRARPWNDVAADLHRGEDILSRANVDVLLVDPQRLPENGVNFRGKNTELGEAAKLIA
                     LEGTRVGYAVQRRFDAMQGWTRLNRGGVGPDDLHMNDDGYACWAAITAEGLAAALL"
BASE COUNT          415 a          827 c          763 g          388 t
ORIGIN      
        1 gtcgtctcgc aggcttcgat cgcggcccag cggcgccgga cgctggttcg cagccggtcg
       61 ccacgcatct tgcccgcctt gttcagaagg ccgaccgccg cgttgctcag gcccgcgctc
      121 aggctcggac gcacgatcac cgcctctcgg acggtctggt gggcattgca gaaatacttc
      181 ttgtagccct caccgccgaa gccgaaatcg aacacgcggt aaccccgggc cgaggccagc
      241 cgcatggtgt ccaggctcag caggataccc ggcgagcacc gcgccagcgt cggctcatag
      301 accgggaacc agaaatggta ccgccccccg gcgtgcagcg aatactccac cgccaccagc
      361 ttgccgcctg cgtacatggc cgccatcgag gctccgaagt cgtcctcgcc ccgcgcctgc
      421 agcagggcgt gcaacaggtc ggcggtccag ccgcagtcga agatgtcgtg gcgtcccgtg
      481 cggcgatatt gatcccgctt cagcacgatc agctgatcca gcagggccgg atcgcgcaga
      541 tcgcgctcga ccgtgatggc tcccagctcg gtctcgagac tgcggcgcgc gcgttccttg
      601 tccttgaaat atttgccgca ggtctggcga cgctctgcat accaggcgcc gtagctgtcg
      661 ccgtccggtc cctccggcat ggcgaccata agggtttcgc gatcttcagc ctcatgcgcc
      721 tcaccgaccc aggcgccgac acgcaggcgt gggacgttca gcagactggc caccgtatcg
      781 aggtccggag cacgaccggg gaaggcgatc accccgtgat agtcgttcat cggtgcgccc
      841 agcggctgga tcgtgcctcc gcgcgtctga tgcggaaaga agccaacgat ctcgccgtcc
      901 tgccggaaca cggccactgc ggcctcgggg ctgatcgacc cggcgatacg ggtgaactcc
      961 ggcctgaaat aggggctggc cagatcaggg tttgcagcca gcatggcgcg ccacagggtc
     1021 tcctccgcag ggctcagcgc ctccggcttg atgatctcga cctgtagcga tccggccatg
     1081 cggttctccg attccagcgg tcacgcttag ccgatcgccg tttcagtacg gtttagcggg
     1141 atggtgaatg acacctttcg acgcgacggt ccggagccga tctgcgatcg ggagcgttgc
     1201 cgcagacggc cagaccgtgg ataatcagga agaccgccat gacagacgcc aatacgccca
     1261 aggatcgtcc taaaaaccgg accctggaaa acgagaacca gcgcaacccg tcctccgacg
     1321 gcgaggcccc gcgcccggcg accgaaccgc ccggctcgga aggttcgagc aactctggca
     1381 agactgccac cgaccccgcg actggagaag aaaacgggtg agactgacga ctgtcctgct
     1441 gaccgccgct gccagcgtcg gccttggctt gctcatttcc ggcgtggcgc gagcccagac
     1501 cgccgagcct ggtacgagcc tgcttgcgtc ggagccggtc atccccgccc cgaccgaggc
     1561 gcccgcgaca gacgcaccct cttgtccggt cagcagcgac agtctggtcc tcgccggcga
     1621 tggcctgaaa cgcagcaagg cggcggtggc tttgaatcgc ctgaacatcc tcgccgtcgg
     1681 atcgtccagc atcgagggcg tgggcgccag caaggccgag ctggggttcg tccccttgct
     1741 gcaaaccgat ctgcgtaacc ggctgcccga tgtggcggtc aacgtctgga acaggggcat
     1801 aggcggcgag actgcgcatg agacggttaa tcggctcgag gccgagattc agcgctttcg
     1861 acccgatctg gtgatctggc agttgggcac caatgacgtg ctgcgcgccc ggccgtggaa
     1921 cgatgtcgcc gccgacctgc atcgcggcga ggatatcctg tcgcgcgcca acgtcgacgt
     1981 cctgctagtc gacccgcagc gcctgcccga gaacggcgtc aacttccgcg gcaagaacac
     2041 cgagctcggc gaagcggcca agctgatagc gctcgaaggc acccgcgtcg gctatgccgt
     2101 ccagcggcgg ttcgacgcta tgcagggctg gacccggtta aaccggggcg gcgtcggccc
     2161 cgacgacctg catatgaatg acgacggcta cgcctgctgg gccgccatca ccgccgaagg
     2221 actagccgcc gcgctgctat aaggcgacgc tgagggaacg ggcgacgatc tggggaattc
     2281 ccagaggccg accagccctt tcgagtgtcc cccgatgcgt cttctgcccg ccctgctcgc
     2341 ctccgccgcc gtgcctatcg tcgcgttttc gatggccgct tgcggatcgc ccg
//
LOCUS       DASVTM010000517         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_742768,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000517 DASVTM010000000
VERSION     DASVTM010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742768"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF633_14180"
     CDS             <1..656
                     /locus_tag="VF633_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX8472255.1"
                     /translation="SQVRSRGPAPETRPRADYRAAANHILAIGASTGGVEALLTILSA
                     FPADCPATVVTQHMPATFTGSFAARLNKMSGATVTEAVDGAPMLPGHIYIAPGGLTHL
                     EVLGATPRCRLSQGDTVSGHRPSVDVLFRSVSRLRRPMTGVILTGMGRDGAQGLLEMR
                     NAGAHTLGQDEASCVVYGMPRVAHEIGAVERQLPLSRLSSAILDLCAAPPRASQSAA"
     gene            667..1056
                     /locus_tag="VF633_14185"
     CDS             667..1056
                     /locus_tag="VF633_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472256.1"
                     /translation="MPAAVSIHTLIVDDQMSMRALIRTSMQQLGFREFREAADGEAGL
                     REMIGKPPHLVISDFNMPKLDGLGLLRAVRAHPPTQGCAFIMLTGRADRELVARAVQF
                     GVNNYLVKPFTVAQLKEKLEGVFGPLT"
     gene            1056..1595
                     /locus_tag="VF633_14190"
     CDS             1056..1595
                     /locus_tag="VF633_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505894.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX8472257.1"
                     /translation="MSAAALASEKRIHVGQGDHHVTSDPDVMLTTILGSCVAMCLRDP
                     GAGVGGMNHFLLPEGAGAGTDAGRRYGAYAMELLINEVLKAGARRDRLEAKLFGGGRM
                     FDSMKNVGLANADFAERFLVDEGIPLVGGSLRGPGGRRLHYWPVSGRALQRAVTDNHV
                     PVAKPPRIQPAASGAVELF"
     gene            1595..1933
                     /locus_tag="VF633_14195"
     CDS             1595..1933
                     /locus_tag="VF633_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472258.1"
                     /translation="MLLNGDPALLSVIADEMADVRLDIDDLSTLLVELTAHCPAEKRA
                     DAVRRTQACDLIIQRLDGLSGLMAALGAGVPWETALHALTLSDQSERLTGRSRPLAAP
                     AESGDLMLFD"
     gene            1936..2535
                     /locus_tag="VF633_14200"
     CDS             1936..2535
                     /locus_tag="VF633_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472259.1"
                     /translation="MAKASPTDRVNLAHSTVLLIDHNSQSLDMLSSIFHGFGVREQIK
                     CGSTEQAVKLVQRRDVDLILIESSMPEMDGYDFIRWLRRETQPPVRHTPVIMLTGHAA
                     QSKIRKSRDCGSSFIVTKPLTPSVLLQRILWLGADERDFVECDTYVGPDRRVRNFGPP
                     LGIPGRRSGDLSEHVGAAKESNMDQASIDMMMKPMRVAL"
     gene            2556..2987
                     /locus_tag="VF633_14205"
     CDS             2556..2987
                     /locus_tag="VF633_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheE"
                     /protein_id="HEX8472260.1"
                     /translation="MTSTLARRLAEPGGRTVRDASRLAGQQLEGHRETVMATIAATLD
                     ELDALALTAAADAGPHVYQLASTMIDLGGFFDTGPLHEASYSLCDVTDRMIAADVWHW
                     PSVRVHLQAMRLILTGGCQASRTSEALLLGLRSVSQRSLTD"
     gene            complement(3004..3597)
                     /locus_tag="VF633_14210"
     CDS             complement(3004..3597)
                     /locus_tag="VF633_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8472261.1"
                     /translation="MPRAAGQIDPRKSEAILEAARVLFHELGGAVSMEAIAKKAGVSK
                     QTLYNRFASKTELARALAEQRSTAITAPLRTNGDPEAVLTALAIALVTKICNAQKGES
                     LRGVALMSPPIPGVAEAIYEGGPAESLRRLAAWLAEQDRKGLLSIPDPAHAAEMFTGM
                     AVGHGHLRALLGLQHPEIDIEARARETAHRFIRAFGR"
     gene            complement(3597..>4502)
                     /locus_tag="VF633_14215"
     CDS             complement(3597..>4502)
                     /locus_tag="VF633_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8472262.1"
                     /translation="LQYVLEEGSKNDWLNDNLILLLALTALIAGPAFVWRSLTYRNPI
                     VELRAFTNRNFMVGVVMTFTVGFALFGGTFLLPLFLGRVRDYSPSEVGTTMVVSGLAM
                     FATAPFAGRLVRTVDARLLMFGGFMMAAWGMWDARLVTDDWGFWEFAGIQALRGAGVM
                     IAMIASQQVTMSTLPPHMVKNASGLVNLFRNVGGAFGLAFLNTSLTTNTAAHMSELTT
                     RISQTNIQMTDMMAGMADRFSNSINPEGAAMKAIYGMMQQQATTLAFGDAFAVLGIGC
                     AIAAFVTLLAKPVKANPAAPPVDAH"
BASE COUNT          774 a         1476 c         1447 g          805 t
ORIGIN      
        1 ggtcccaggt ccggtcaagg ggaccggccc cggaaacccg gccgcgtgcc gactatcgcg
       61 ccgctgccaa ccacatcctg gcgatcggag cctctacagg cggggtcgag gccttgctca
      121 cgatcctctc ggccttcccc gcagactgcc ccgcaacggt agtgacgcag catatgccag
      181 ccacgttcac cggcagcttc gccgcgagac tgaacaagat gtcgggcgcc acggtcaccg
      241 aagccgtcga cggggccccc atgctgccag gccacattta cattgcgccg ggcggcctga
      301 cccacctgga agtcctgggc gcgactccgc gttgtcgtct gagtcagggc gacaccgtca
      361 gcggccaccg tccttccgtt gacgttctgt tccgctcggt ctcccgcctg cgccgcccga
      421 tgacgggcgt gatcctgacc ggcatgggtc gggacggagc tcagggcctg ctcgaaatgc
      481 gcaatgccgg cgcccatacg cttggtcagg atgaggccag ttgcgtcgtc tacggcatgc
      541 cgcgggtcgc gcatgagatc ggcgccgtag aacgccagtt gccgttgtct cgtctttcgt
      601 ccgctattct cgatctgtgc gccgcgcctc cgcgcgcctc acagtccgcc gcctgaggtt
      661 tcccccatgc ccgccgcagt ttccatccac accctcatcg tcgacgatca gatgtcgatg
      721 cgcgccctga ttcgcaccag catgcagcag ctgggattcc gtgagttccg cgaagcggct
      781 gatggggagg ctggtctccg cgagatgatc ggcaaacctc cccatctggt gatctcggac
      841 ttcaacatgc ccaagctcga cgggcttggc ctgctacggg ccgtccgggc ccatccgccg
      901 acgcagggct gcgccttcat tatgctgacc ggccgcgcag atcgcgaact ggtcgcgcgc
      961 gcagtgcagt tcggagtcaa caactacctc gtcaaaccct tcaccgtcgc ccagctcaaa
     1021 gagaagctgg aaggcgtttt tggtcctctc acatgatgtc agcggcggct ctggcgagcg
     1081 aaaaacggat acacgtgggc cagggcgatc accatgtgac gtccgatccg gacgtcatgc
     1141 tgacaacgat cctgggcagc tgcgtggcga tgtgtctgcg cgatcccggc gccggcgtcg
     1201 gcggcatgaa ccatttcctg cttccggagg gtgcgggcgc cgggaccgac gcaggccgtc
     1261 gttacggcgc ctatgcgatg gagctcctga tcaacgaagt gctcaaggcc ggggcgcgtc
     1321 gcgaccgcct ggaagccaag ctcttcggcg gcgggcgcat gtttgattcc atgaagaacg
     1381 tgggtctggc caatgccgac ttcgcagaac gtttcctcgt ggatgagggc atcccgcttg
     1441 tcggcgggag tttgcgcggg cccggtggtc gacgactgca ctactggccg gtcagcggac
     1501 gtgcgctgca gcgcgccgtc accgacaatc atgtgcccgt tgccaagccg ccgcgcatcc
     1561 agcccgccgc ttccggcgct gtggagttgt tctgatgctc ttgaacggcg atcccgccct
     1621 gctcagcgtc atcgccgacg agatggcgga tgtgcgcctc gatatcgatg atctctccac
     1681 cctgttggtc gagttgactg cccattgccc ggcggagaag cgcgccgacg ccgtcagacg
     1741 gacccaggcc tgcgacctca tcatccagcg actggacggc ctcagcggcc tgatggccgc
     1801 tcttggcgcc ggtgtgccgt gggaaaccgc gcttcacgcc ctcaccttgt ccgatcagtc
     1861 cgagcgtttg accgggcgct cgaggcccct cgccgctccg gcggagtccg gcgatctgat
     1921 gctgttcgac tgacgatggc caaggcttct ccgacagaca gggtcaatct cgcgcattcg
     1981 accgtgctgc tgatcgatca caacagccag tcgctcgaca tgctgagttc gatcttccac
     2041 ggcttcgggg tacgcgaaca gatcaagtgc ggatcgaccg agcaggccgt caagctcgtc
     2101 cagcggcgcg acgtcgacct tattctgatc gagtcttcca tgcctgagat ggacggctat
     2161 gacttcatcc gctggttgag gcgcgaaacc cagccgccgg tgcgtcatac ccctgtgatc
     2221 atgctgaccg ggcacgcggc gcagtcaaag attcgcaaga gccgcgactg cgggtccagc
     2281 ttcatagtca ccaagccact gacgccatcg gtcctgctgc agcgcatact gtggctcgga
     2341 gcggacgaac gggacttcgt ggaatgtgac acctatgtgg gacccgaccg ccgggtgcgg
     2401 aatttcgggc cgcctttggg gatccccgga cgccgttcgg gagatctgtc cgaacatgtc
     2461 ggcgccgcca aggaaagcaa tatggaccag gcgtcgatcg acatgatgat gaagccgatg
     2521 agggtcgccc tgtgaccgaa acacgaacat acaaggtgac gagcacgctg gcgcgccggc
     2581 ttgccgagcc tggcgggcgt acggtccggg atgcttcgcg cctcgccgga cagcagctgg
     2641 aaggccatcg cgagactgtt atggcgacga tcgccgccac tctcgacgaa ctcgacgccc
     2701 tcgccttgac tgccgcggcg gatgcggggc ctcacgtcta tcagctcgcg tcgaccatga
     2761 tcgatcttgg cgggttcttc gacacggggc cgctacacga ggccagctac agcctgtgcg
     2821 acgtgaccga caggatgatc gctgccgacg tctggcactg gccatcggtt cgagttcacc
     2881 ttcaggccat gcggctcatc ctgaccggag gatgccaggc cagccgcacc agcgaagctc
     2941 tgctgttggg cctgcgctcc gtcagccaga ggtctttgac ggactgacag acgacgcgag
     3001 cgatcagcgc ccgaaggccc ggatgaagcg gtgcgcggtt tcgcgggcgc gagcctcgat
     3061 gtcgatctcg ggatgctgaa gacccagtaa cgcgcgcaag tgaccatgac cgacagccat
     3121 gccggtgaac atctcggcgg cgtgggccgg gtcggggatc gatagcaggc ccttgcggtc
     3181 ctgttcggcc agccaggccg ctagtcggcg cagactctcg gccgggccgc cttcatagat
     3241 ggcctctgcg acaccgggga tcggaggcga catcagagcg acgccgcgca ggctttcacc
     3301 cttctgggcg ttgcagatct tggtgacgag ggcgatggcg agcgccgtca gcaccgcctc
     3361 cgggtcgccg ttggttcgca ggggtgcggt tatggctgtc gacctctgct ccgccagcgc
     3421 ccgggccaac tccgtctttg aagcgaaccg gttgtacaga gtctgtttgg agaccccagc
     3481 ctttttggcg atcgcctcca tcgacacggc tccgcccaac tcatggaaca gcacacgggc
     3541 cgcctcgagg atggcttcgc tcttgcgggg gtcgatctgg ccggcggctc tcggcatcag
     3601 tgggcgtcca ccggaggcgc ggcggggttg gcctttaccg gcttggccag cagggtgacg
     3661 aacgccgcga tggcgcagcc gatgccgagc actgcaaagg cgtcgccaaa cgccagcgtc
     3721 gtcgcctgct gctgcatcat gccgtagatc gccttcatcg ccgcaccctc ggggttgatc
     3781 gagttgctga agcggtcggc catgccggcc atcatgtcgg tcatctggat gttggtctgg
     3841 ctgatgcggg tcgtcagctc gctcatgtgt gcggctgtgt tggtcgtcag cgaggtgttc
     3901 aggaaggcga ggccaaaggc gccgccaacg ttgcggaaca ggttcaccag cccggaggcg
     3961 ttcttcacca tgtgcggtgg cagcgtcgac atcgtcacct gctgcgaggc gatcatggcg
     4021 atcatgacac cggccccgcg caacgcctga atgccggcga actcccagaa gccccagtcg
     4081 tcggtgacca gacgcgcgtc ccacatgccc catgcggcca tcatgaagcc cccgaacatc
     4141 aacaaacgtg cgtcgaccgt acgtaccaac cgcccggcaa acggagccgt ggcgaacatg
     4201 gcgagccccg acaccaccat cgtcgtgccg acctcggacg gcgaatagtc acgcacccga
     4261 ccgaggaaca gcggcaacag gaaggtcccg ccgaacaggg cgaaaccgac ggtgaaggtc
     4321 atgacgaccc cgaccatgaa gttgcggttg gtgaaggctc gaagttcgac gatagggttt
     4381 cggtaggtca acgatcgcca gacgaaggct ggccccgcga tcagggcggt cagggccagc
     4441 agcaggatga ggttgtcatt gagccagtcg ttctttgagc cttcctccag cacgtactgg
     4501 ag
//
LOCUS       DASVTM010000518         5014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_264127,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000518 DASVTM010000000
VERSION     DASVTM010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264127"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /gene="uvrC"
                     /locus_tag="VF633_14220"
     CDS             <1..1813
                     /gene="uvrC"
                     /locus_tag="VF633_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX8472263.1"
                     /translation="ARTAPETAGVYRMLGEGGEVLYVGKAKNLKKRVVNYTQVMKLPT
                     RLQRMVAETTTMEFVTTHTEVEALLLESNLIKRLMPRYNVLLRDDKSFAELMIRRDHP
                     VAQIRKHRGQHTTQGDYFGPFASTWAVNRTLNTLQKAFLLRSCSDSVYETRTRPCMLH
                     QIKRCSAPCTGLISLEDYGELVTEAEQFLRGKSRAVIGRLSADMTAAAEEMDFEQAAR
                     VRDRIRALSAISMENSVSADSVAEADVFALFSEGGQACVQVFFYRAGQNWGGRAYFPR
                     VDRSDTDPEILGAFLGQFYEDKPVPRLILSNVRPHELELLEEAFTMKAERKVEINRPQ
                     RGEKLALVDHAHTNAREALGRKMAEGSAQGKILDEVCEAFGLETRPERIEVYDNAHIS
                     GTNAVGGMIVAGPEGFQKSQYRKFNIRDQDITPGDDYGMMREVMRRRFARLVKDEEAG
                     EEDVVRPDLLLIDGGAGQLAETLAVLADLGLDDILAVGVAKGPDRDAGKEHFFMPGKP
                     PFMLPLKSPALYYIQRLRDEAHRFANGAHAKRRSMDIKKNPLDEIEGVGPGRKRALLH
                     AFGSAKGVSRASVSDLVKVDGINEPLAERIHGFFHTA"
     gene            1824..2441
                     /locus_tag="VF633_14225"
     CDS             1824..2441
                     /locus_tag="VF633_14225"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX8472264.1"
                     /translation="MNPDDELTAGYLRFRKDHWPAARADYENLAKGQKPHTLIVACSD
                     SRADPALIFDAAPGELFVVRNVANLVPPYEPDGQLHGVSAALEFGVKVLEVSRIVVMG
                     HAHCGGVNAMLNGAPDNCRDFVAPWVEQGAPVVRHVAETVAPEEVESAAEEAVVRLSI
                     RNLRTFPWIAEREAAGELTLSGLHFGIADGVLRALGDGPRFEPLG"
     gene            2505..3164
                     /gene="pgsA"
                     /locus_tag="VF633_14230"
     CDS             2505..3164
                     /gene="pgsA"
                     /locus_tag="VF633_14230"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX8472265.1"
                     /translation="MSATPHPVNPIPNILTGIRLLAGVVMFLILAGATGGFPFLSAYL
                     DPDDQFALYRTAFWIFVVAASTDWVDGYLARRWNATTRWGAILDPIADKILVTGAILG
                     VLTSGSVPQIAIPCGLILFREFAVSALRETVAGKVKLDVTLLAKWKTTLQMVALGAQL
                     LARNWDGFGWDLEYIDEFQIFADTLIWAAAIATLWTGWQYFDKARREMSRPDVVKLDP
                     L"
     gene            complement(3161..3688)
                     /locus_tag="VF633_14235"
     CDS             complement(3161..3688)
                     /locus_tag="VF633_14235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8472266.1"
                     /translation="MSGVVFRPADEADVEALHALVTSAYRGDAARLGWTHEADLLDGQ
                     RTDVAALSEVIADSSKVILMAHHGGVLIGCVMLAQQHDGSAYLGMLSVDPVRQASGLG
                     RLLLAAAEAEASARYGAGRVEMTVIRQRPELIAWYERRGYALTGATAEFPMDDERFGL
                     PKRRDLEFVVLSKTL"
     gene            complement(3685..>5014)
                     /locus_tag="VF633_14240"
     CDS             complement(3685..>5014)
                     /locus_tag="VF633_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX8472267.1"
                     /translation="VSGSPGATQRLLTQDQLMTVRDVVLPMDQLIASELRGRLIRYSE
                     EGERHAFEAMDPIVGLENTYKRGLGRMRALTDPGFMATRAAGEVDFRQKAGEQVFNDL
                     AGIQPAVRELYAPYALLEGGNGIGTTPVAGGSQLFLWARALVRGAQERAKPSDQRLPE
                     FGDSRLSATQTALFAERPVYPELEQIRMEWWLSKTREWLTLDSPYVKPLLGQESPEQL
                     SARIVSGTKLADPAVRHALWDGGLAAIQASNDPMIQYLLAIQEPTRAIRTEWEEKVQA
                     PTDRASEQLATARFAAYGDAVYPDATGTLRLTYGKIEGSDVPAQRWGPFTTFAGLWDR
                     ATGAAPFDVAPRLLAAKDRIPADTVLDMTVSSDTIGGSSGSPVVNAKGEIVGANFDST
                     VLSQRNAYGYDRNVNRSVIVTTGAVTAALRDVYGMSGLVAELGGQSAPAR"
BASE COUNT          861 a         1648 c         1645 g          860 t
ORIGIN      
        1 cgccaggacc gcgccggaga ccgccggcgt ctatcggatg ctgggggagg ggggggaggt
       61 cctctacgtg ggcaaggcca agaacctgaa gaagcgggtg gtcaactaca cccaggtcat
      121 gaagctgccg acccgccttc agcggatggt ggcggaaacg acgacgatgg agttcgtcac
      181 gacccatacc gaggtcgagg cgctgctgct cgagtcgaac ctgatcaagc ggctgatgcc
      241 gcgctacaac gtgctgctgc gcgacgacaa gtccttcgcc gagctgatga tccgccgcga
      301 ccacccggtg gcccagatcc gcaagcaccg cggccagcac acgacgcagg gcgactactt
      361 cggccccttc gccagcacct gggcggtgaa ccgcacgctg aacaccctgc agaaggcctt
      421 cctgctgcgc tcgtgctcgg acagcgtcta cgagacccgg acgcgcccct gcatgctgca
      481 ccagatcaag cgctgctcgg cgccctgcac cgggctgatc tcactggagg actatggcga
      541 gttggtcacc gaggccgaac agttcctgcg cggcaagtcg cgcgcggtga tcgggcggct
      601 ctcggccgat atgaccgccg ctgccgagga gatggatttc gaacaggcag cgcgtgtccg
      661 tgaccgcatt cgcgccctgt cggccatttc gatggagaac tcggtctccg cggacagcgt
      721 cgccgaggct gacgtcttcg ccctgttctc cgaaggcggg caggcctgtg tgcaggtctt
      781 cttctaccgc gcaggccaga actggggcgg gcgcgcctat ttcccgcggg tcgaccgctc
      841 cgataccgat ccggaaatcc tgggagcgtt cctcggtcag ttctacgagg acaagccggt
      901 accacgcctg atcctgtcca acgttcgtcc gcacgaactg gaactgctcg aggaagcctt
      961 cacgatgaag gccgaacgca aggtcgagat caatcgcccg cagcgcggcg agaagctggc
     1021 cttggtcgac cacgcccaca ccaacgcgcg cgaagccctg ggtcgcaaga tggccgaggg
     1081 ctcggcgcag ggcaaaatcc tcgacgaggt ctgtgaggcc ttcggtctgg aaacgcgtcc
     1141 ggaacggatc gaggtctacg acaacgccca catctccggc accaatgccg tcggtggcat
     1201 gatcgtcgcc gggccggaag gcttccagaa atcgcaatat cgcaagttca acattcgcga
     1261 tcaggacatc acgcccggcg acgactacgg catgatgcgc gaggtcatgc gtcgccgttt
     1321 cgcccgtctg gtcaaggacg aggaagccgg tgaggaagac gtcgttcgcc ccgacctgct
     1381 gctgatcgac ggcggggcag ggcaactggc cgagacgctg gcggtgctgg ctgacctggg
     1441 gctggacgac atcctggccg tcggggtggc aaaagggccg gaccgtgacg cgggcaagga
     1501 gcatttcttc atgcccggca agccgccctt catgctgccg ctgaagtcac cggcgctcta
     1561 ttacatccag cgtctgcgcg acgaggccca ccggttcgcc aacggcgccc acgccaagcg
     1621 ccgctcgatg gacatcaaga agaatccgct ggacgagatc gagggcgtcg gtccgggtcg
     1681 caagagggcg ctgctgcacg cgttcggctc ggccaagggc gtgtcgcgcg cctccgtgtc
     1741 cgatctggtc aaggtcgatg ggatcaacga accgctcgcg gagcgtattc acggcttctt
     1801 ccacacagcg tagaagcctc gccatgaacc ctgacgatga actgaccgct ggctacctgc
     1861 gcttccgcaa ggatcattgg ccggctgcgc gcgccgatta cgaaaacctc gccaagggcc
     1921 agaagccgca caccctcatc gtggcctgtt cagatagccg cgcggatccc gccctgatct
     1981 tcgacgcggc tccgggcgag ctgttcgtgg tccgcaacgt cgcgaatctc gttccgcctt
     2041 atgagcccga cggccagttg cacggggtgt ccgctgcgct cgagttcggg gtcaaggtgc
     2101 tggaagtatc gcgcatcgta gtgatgggcc atgcccactg cggcggcgtc aacgccatgt
     2161 tgaacggcgc ccccgacaac tgccgggact tcgtcgcacc gtgggtcgag cagggcgccc
     2221 cggtggttcg tcatgttgct gagacggttg cgcccgaaga ggtcgagagc gccgcggagg
     2281 aggccgttgt gcgcctgtcc atccgcaacc tgcgtacctt cccgtggatt gcagaacgcg
     2341 aagccgctgg agaactgacc ctgagtgggc tgcacttcgg tatcgccgac ggggtattgc
     2401 gcgccttggg cgacggtccc cggttcgaac ctctgggttg atcgagcgtt cgcctctgac
     2461 ggcgaagcat ggcacaccca cgcagccgac tgaaaagaga cccgatgagc gccaccccac
     2521 accccgtcaa tccgatcccc aacatactga ccggcattcg tctgctggca ggcgtggtga
     2581 tgttcctgat cctggctgga gccacgggcg ggttcccgtt cttgtccgcc tatctggatc
     2641 ctgacgacca gttcgccctt taccggaccg ccttctggat cttcgtggtc gcggcctcaa
     2701 ccgactgggt cgatggctat ctggctcggc gctggaacgc gaccacgcgt tggggtgcga
     2761 tcctcgatcc gattgccgac aagatccttg tcacaggcgc catcctcggc gtgctgactt
     2821 cgggctcggt gccgcagatc gccattccct gcggcctgat cctgttccgc gagttcgccg
     2881 tctcggcgct gcgcgaaacg gttgcgggca aggtgaagct ggatgtcacc ttgctggcca
     2941 agtggaagac gaccctgcaa atggtggcgc tcggtgcgca attgttggcc cgcaattggg
     3001 acggtttcgg ctgggacctc gaatatatcg acgagttcca gatcttcgcc gacaccctga
     3061 tctgggccgc cgccatcgcg accctttgga ccggctggca gtatttcgac aaggctcgcc
     3121 gcgagatgag ccgccctgat gtcgtgaaac tggatccgct ctagagggtc ttcgatagca
     3181 ctacgaactc gagatcgcgt cgtttcggca ggccgaaccg ctcgtcgtcc atcgggaatt
     3241 cagccgtcgc gcccgtcagc gcatagccgc gtcgttcgta ccaggcgatc agctctggcc
     3301 gttggcggat gacggtcatc tcaacacgac cggcgccgta gcgcgcactg gcttcagcct
     3361 cggcagcggc cagaaggagg cggcccaggc cgctggcctg tctcaccggg tcgactgaca
     3421 gcatgcccag ataggcggat ccgtcgtgtt gctgcgcgag catcacgcag ccgatcagaa
     3481 caccgccgtg gtgcgccatc aggatcacct tcgaagaatc agcaatgacc tcggaaaggg
     3541 ccgcgacgtc ggtgcgctgg ccgtccagca ggtcggcctc atgggtccag ccgagacggg
     3601 cggcgtcccc gcgataggcg ctggtgacca gtgcatgcag cgcctcgacg tcggcctcgt
     3661 ccgcgggccg aaatacgacg ccgctcaccg ggcgggggcc gactgtccgc ccagttccgc
     3721 aaccagtccg ctcatgccgt aaacgtcgcg cagggccgcg gtcaccgcac ccgtcgtgac
     3781 gatgacgctg cggttgacgt tgcgatcgta gccataggcg ttgcgctggc taagcacggt
     3841 cgagtcgaag ttcgccccga cgatctcacc tttcgcgttg acgaccggcg agcccgagga
     3901 cccgccgatg gtgtccgaag agacggtcat gtccagcaca gtgtcggccg ggatccggtc
     3961 cttggcggcc agcagcctgg gcgcgacatc gaaaggcgcg gcgcccgtcg cgcggtccca
     4021 caggcctgca aacgtcgtga acgggcccca gcgctgggcg ggcacgtcgg agccttcgat
     4081 cttgccgtag gtcaggcgca gcgtgccggt tgcgtcggga tagacggcgt cgccgtaggc
     4141 ggcgaaacgg gcggtcgcga gctgttccga ggcgcggtcg gtcggcgcct gaaccttttc
     4201 ttcccattcg gtgcggatgg cgcgggtcgg ctcctgaatc gccagcagat actggatcat
     4261 cggatcgttg gaggcctgaa tggccgccag acccccgtcc caaagggcgt ggcgtacggc
     4321 ggggtcggcc agttttgtgc ccgatacgat gcgcgccgac agctgttcag gcgactcctg
     4381 gcccagcagc ggcttcacat agggactgtc gagagtcagc cattcgcggg tcttggacag
     4441 ccaccattcc atccggatct gctcaagctc cggatagacg ggacgctcgg cgaacagggc
     4501 ggtttgcgtg gcggacaggc gcgagtcccc gaactccggc aggcgctgat ccgacggctt
     4561 ggcccgctcc tgagcgccac gcaccagcgc ccgcgcccac aaaaacagct gcgacccacc
     4621 ggcgaccggg gtcgtgccga tgccgttccc accttccagc agggcgtagg gggcgtacag
     4681 ttcgcggacc gcaggctgga ttccggcgag gtcgttgaag acttgctcgc ctgccttctg
     4741 ccggaaatcg acttcgccag ccgctcgtgt cgccatgaag ccgggatcgg tcagggcgcg
     4801 cattcggccg aggccacgct tgtaggtgtt ctccagcccg acgatcggat ccatggcctc
     4861 gaacgcgtga cgctcgcctt cttcggaata gcggatcagc cgaccgcgca gctcggaggc
     4921 gatcagttgg tccataggca ggaccacgtc gcgcaccgtc atcaactggt cctgcgtcag
     4981 cagccgctgg gtcgccccgg ggctacccga gacg
//
LOCUS       DASVTM010000519         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_211103,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000519 DASVTM010000000
VERSION     DASVTM010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211103"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VF633_14245"
     CDS             <1..1398
                     /locus_tag="VF633_14245"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX8472268.1"
                     /translation="SLGCWHGFIGQQKMISIKKHFGTTDRKYLYLSGWMIAALRSDFG
                     PLPDQSMHEKTSVPALIEELYTFLRQADARELGGLFRDIDAAREAGDAVTERKLLDAV
                     DNYQTHVVPIIADIDAGFGNAEATYLLAKKMIEAGACALQIENQVSDEKQCGHQDGKV
                     TVPHEDFLAKVRACRYAFLELGVEDGVIVTRTDSLGAGLTKQIAVSNEPGDLGDQYNS
                     FLDCEEISAAELKNGDVVINRGGKLLRPKRLPSNLFQFRAGTGEDRCVLDSITSLQNG
                     ADLLWIETEKPHVEQIAGMMDRVREVIPNAKLVYNNSPSFNWTLNFRQQVFDTMQAEG
                     QDVSGYDRAKLMSAAYDETPLAVQADEAIRTFQSDGAKRAGIFHHLITLPTYHTAALS
                     TDNLAREYFGAAGMLGYVKNVQREEIRQGIACVKHQNMSGSDIGDDHKEYFAGEAALK
                     AGGAHNTMNQFGEAA"
     gene            complement(1477..>1831)
                     /gene="ileS"
                     /locus_tag="VF633_14250"
     CDS             complement(1477..>1831)
                     /gene="ileS"
                     /locus_tag="VF633_14250"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288073.1"
                     /note="IleRS; catalyzes the formation of
                     isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since
                     isoleucine and other amino acids such as valine are
                     similar, there are additional editing function in this
                     enzyme; one is involved in hydrolysis of activated
                     valine-AMP and the other is involved in deacylation of
                     mischarged Val-tRNA(Ile); there are two active sites, one
                     for aminoacylation and one for editing; class-I
                     aminoacyl-tRNA synthetase family; some organisms carry two
                     different copies of this enzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX8472269.1"
                     /translation="ARWAKVQTVLETVNEQLEGARREKTIGGALDAWPVVTGPASAFE
                     AYADLDAAEVFRTSGAELVVGGDAVTVEVKLADYPKCARSWRRVPDVGSDAAYPELSA
                     RDADAVRFWDQTHAS"
BASE COUNT          364 a          632 c          540 g          295 t
ORIGIN      
        1 tcgctgggct gctggcacgg cttcatcggc caacagaaga tgatctcgat caagaagcat
       61 ttcggcacga ccgaccggaa atacctctac ctgtccggct ggatgatcgc ggccctgcgc
      121 tcagactttg gccccctgcc cgaccagtcg atgcacgaaa agacttcggt cccggccctg
      181 atcgaggagc tctacacctt cctgcgtcag gctgacgccc gtgagctggg cggcctgttc
      241 cgcgacatcg acgccgcccg cgaggccggc gatgccgtca ccgagcgcaa gctgctggac
      301 gccgtcgaca actatcagac ccacgtcgtg ccgatcatcg ctgacatcga cgccgggttc
      361 ggcaacgccg aggcgaccta tctgctggcc aaaaagatga tcgaggccgg cgcctgcgcg
      421 ctgcagatcg agaaccaggt ctccgacgag aagcagtgcg gtcaccagga cggcaaggtc
      481 acggtcccgc acgaggactt cctcgccaag gtccgcgcct gccgctacgc cttcctagaa
      541 ctgggcgtcg aggatggcgt catcgtcacc cgcaccgaca gcctcggcgc cggcctgacc
      601 aaacagatcg cggtctcgaa cgaacctggc gatctgggcg accagtacaa cagcttcctc
      661 gactgcgagg aaatctcggc cgctgaactg aagaacggcg acgtcgtcat caaccgcggc
      721 ggcaagctgc tgcgtcccaa gcgcctgcct tcgaacctgt tccaattccg cgccggaact
      781 ggcgaggatc gctgcgtcct cgacagcatc acctcgctgc aaaacggcgc cgacctgctg
      841 tggatcgaga ccgagaagcc gcacgtcgaa cagatcgcgg gcatgatgga ccgcgtccgc
      901 gaggtcatcc ccaacgccaa actggtctac aacaactcgc cctcgttcaa ctggacgctc
      961 aacttccgcc agcaggtgtt cgacactatg caagctgaag gtcaggacgt ctccggctac
     1021 gaccgggcca agctgatgag cgcagcctat gacgagaccc cgctggcggt tcaggccgac
     1081 gaagccattc gcacgttcca gtcggacggc gcgaaacggg cgggcatctt ccaccacctg
     1141 atcaccctgc cgacctatca cacggccgcc ctgtccacgg acaatctggc ccgcgaatat
     1201 ttcggcgcgg cgggcatgct gggctacgtc aagaacgtgc agcgcgagga gatccgtcag
     1261 ggcatcgcct gcgtgaagca ccagaacatg tccggctcgg acatcggcga cgaccacaag
     1321 gaatacttcg ccggtgaagc cgccctgaag gccggcggcg cccacaacac gatgaaccag
     1381 ttcggcgaag ccgcctgatc catcggctag gctgaaacgg aaaccggccc ggagcgcatg
     1441 ccccgggccg gcttggtttt ttacagatgc gtcggtctag ctcgcgtgcg tctgatccca
     1501 gaagcggacc gcatcggcgt cccgcgcact gagctcaggg taggctgcat ccgacccgac
     1561 gtccggcaca cggcgccatg agcgagcgca tttcggatag tcggccaatt tgacctcaac
     1621 cgtcacagcg tcacctccga cgacgagttc ggctcctgat gtgcggaaca cctcagcggc
     1681 gtccagatcg gcataggctt cgaatgcgct ggccggtccc gtcaccaccg gccatgcgtc
     1741 cagcgcacca ccaatggttt tctcgcgacg agcgccctca agctgttcgt tgaccgtttc
     1801 cagcaccgtc tggaccttgg cccagcgagc g
//
LOCUS       DASVTM010000520         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1005431,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000520 DASVTM010000000
VERSION     DASVTM010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005431"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1087)
                     /gene="purM"
                     /locus_tag="VF633_14255"
     CDS             complement(56..1087)
                     /gene="purM"
                     /locus_tag="VF633_14255"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX8472270.1"
                     /translation="MSDTPKTLENGLSYADAGVDIDAGERLVDAIKPLAKSTRRPGAE
                     ASLGGFGALFDLKAAGFIDPLIVATTDGVGTKLKIAIETGRHDGVGIDLVAMCVNDLL
                     AQGAEPLLFLDYYATGKLEIDAARRVVAGIAEGCRQAGCALVGGETAEMPGMYSGNDY
                     DLAGFSLGAVERGHALPYLDRQFAGDVIIGLASSGPHSNGYSLIRKVVEKSGLAWGDD
                     APFAKDRSLAQALMEPTKIYIKPVLPLMKAGLIKGAAHITGGGLIENPPRCIAEGLEA
                     SFDWNAWPMPAVFKWLGEVGGISDHELRRTFNCGVGFMLIVAPENVEAVLEGLLEAGE
                     TAFVCGQLV"
     gene            1166..2314
                     /gene="rnd"
                     /locus_tag="VF633_14260"
     CDS             1166..2314
                     /gene="rnd"
                     /locus_tag="VF633_14260"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8472271.1"
                     /translation="MTPITTNDELAAFCKAVAQDSFIAVDTEFMRETTYWPKLCLIQA
                     ATPTHEAIIDPLAEGLDLTPFLDILRDPAIIKVFHAARQDTEIFVKLGAMPKPMFDTQ
                     VAAMAAGFGDQVSYDSLVRQMLKIDLDKGSRFTDWSRRPLSEAQLVYAIGDVTHLAAL
                     YPKLRERLAKEDRLDWVTSEMEDLVDPALYDTTPDNAWKRLKPKKYAAKYLAAFKATA
                     VWREQAAQDRDQPRGRILKDDAIDEIATQTPTDVEAFNRLRAVPKGFGGSRLGLELIE
                     ELKRVLADPEAHAPKLDRPSHNQPAPPSVVELLKVLLKAKSDNAGVASKLIANVADLE
                     KIALSDNAEVDAMHGWRRQLFGEDALKLKRGEIALVLNGARVEVVEIE"
     gene            complement(2318..2674)
                     /locus_tag="VF633_14265"
     CDS             complement(2318..2674)
                     /locus_tag="VF633_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8472272.1"
                     /translation="MAVTGIGGLFFRSKDPKALGAWYLEHLGVGAPEGQYVWNQQAGP
                     TVFSPFKADTDYFPAEKQWMLNLRVDDLDGMMASLRAAGVEIITKAEWDSPEVGRFAR
                     LQDPEGNQVELWQPAP"
     gene            complement(2716..4203)
                     /locus_tag="VF633_14270"
     CDS             complement(2716..4203)
                     /locus_tag="VF633_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX8472273.1"
                     /translation="MNEPWNTPREAAVVDIGSNSVRLVLYRLEGRAIWTVFNEKVLAG
                     LGRDLPATGKLSPNGIEMALTALRRFAVVLESVRPAYVFVAATAAVREALDGPDFCSR
                     VAAETGLQVRVLTGEEEARYSALGVLAGDPTAEGVVADMGGSSLELIRLEGKTLKPGI
                     TLPLGPFSLADPAGFDADRLRSTIAARLTPAAKAFATDSLNAVGGGWRSLAQLHMAMN
                     RYPIRIVHQYAMNADDAREVARLVAQQSRASLDRLPGVSKKRAETLPYSALVLEGLID
                     TLGIKTINFSAWGLREGLLFEALDRDEADEDPLIAGCAALGGRQGISPTLPGALRGWL
                     ENVIAALPTAFGSTRDAVLVSAACQLADLGARLHPDHRLELVFDQVLRAPIPGQTHAE
                     RTFLACVLNARYGGSVATPDPAVVERLLTETTRLRARALGLAMRLGCDLSGRSPQLLA
                     NATVRVDKGVLKLTASEGYADALLGEQTRRRAKALAEVMKLKLDM"
BASE COUNT          758 a         1348 c         1423 g          683 t
ORIGIN      
        1 ctccgtgttc agcggcaatc taaacacatc cctctcccgc cgggagaggg taagattaaa
       61 ccaactgtcc gcagacgaac gccgtctcgc cggcttcgag caagccttcc agcaccgctt
      121 cgacgttctc gggcgccacg atcagcatga agccgacgcc gcagttgaag gtgcggcgga
      181 gttcgtggtc ggagatgccg ccgacttcgc ccagccactt gaacacggcg ggcatcggcc
      241 aggcgttcca gtcgaacgag gcttcaaggc cctcggcgat gcaacggggc gggttctcga
      301 tcaggccgcc gccggtgatg tgcgcggcgc ccttgatcag gccggccttc atcagcggca
      361 ggaccggctt gatgtaaatc ttcgtcggct ccatcagcgc ctgcgctagc gagcggtcct
      421 tcgcaaacgg cgcgtcatcg ccccatgcca gaccggactt ttcgaccacc ttgcggatca
      481 gcgaatagcc gttggagtgc gggccggacg aagccagacc gatgatcacg tcgcccgcga
      541 actggcgatc cagatagggc agggcgtggc cacgctcgac ggcgcccagc gagaagccgg
      601 cgagatcgta gtcgttgccc gaatacatgc cgggcatttc agcggtctca ccgccgacga
      661 gggcgcaacc ggcctgacgg cagccttcgg cgatgccggc gacgacgcgg cgggcggcgt
      721 cgatctcgag cttgccggtc gcataatagt cgaggaacag cagcggctcc gcgccctggg
      781 ccagcaggtc gttgacgcac atggctacga ggtcgatgcc gacgccgtca tggcggccgg
      841 tctcgatggc gatcttgagc tttgtaccga ctccgtcggt cgtggcgacg atcagcggat
      901 cgataaaacc agcggccttg aggtcgaaca gggcgccgaa tccccccagc gaagcctcgg
      961 ctccggggcg gcgagtggac ttcgcgagtg gcttgatggc gtccaccaga cgctctccgg
     1021 catcgatgtc cacgcccgca tcagcgtagc tcaggccgtt ttccagcgtt ttgggtgtgt
     1081 cgctcatgga tcgcgggcct gaatcagtgc ggaaatgtgt tgcggctctt gccacaaagg
     1141 cgtcgcgcgg ggctatagca tcccaatgac gccaatcacc accaatgacg aactggccgc
     1201 attctgtaag gccgtcgccc aagacagttt catcgccgtg gacaccgagt tcatgcgcga
     1261 gaccacctac tggcccaagc tgtgcctgat tcaggccgcg acgccgacgc acgaagcgat
     1321 catcgatccc ttggccgagg ggctggatct gacgccgttc ctcgacatcc tgagggaccc
     1381 ggcgatcatc aaggtgttcc acgccgcacg tcaggacacg gaaatcttcg tcaagctggg
     1441 cgcgatgccc aagccgatgt tcgataccca agtcgccgcc atggccgccg ggttcggaga
     1501 tcaggtcagc tatgattcgc tggtccgcca gatgttgaag atcgatctcg acaaaggcag
     1561 tcgttttacc gactggtcgc gccgtccgct gtcagaggcg caactggtct atgcgatcgg
     1621 cgacgtgacc cacctcgcgg cgctgtatcc caagctgcgc gagcggctgg ccaaggaaga
     1681 ccgtcttgac tgggtgacgt cggagatgga ggatctggtc gatccggccc tctatgacac
     1741 cacgccggac aacgcctgga agcggctcaa acccaagaaa tatgcagcca aatatctggc
     1801 ggcgttcaag gcgacggcgg tgtggcgcga gcaggccgct caggatcgcg accagccacg
     1861 cgggcgcatt ctcaaggacg acgccatcga cgagatcgcg acgcagaccc ccactgacgt
     1921 cgaagccttc aaccgcctgc gcgccgtgcc caagggcttt ggcggctcgc gtctggggct
     1981 ggagctgatc gaagagctga agcgcgttct ggccgatcca gaggcccatg cgcccaagct
     2041 ggatcgcccg tcgcacaacc agcctgcgcc gccgtcggtc gtcgaactgc tcaaggtgct
     2101 gctaaaggcc aagagcgaca atgcaggcgt ggcgtccaag ctgatcgcca atgtcgcgga
     2161 ccttgagaag atcgcgctgt cggataatgc cgaggtcgac gccatgcacg gctggcgccg
     2221 tcagttgttc ggggaggatg cgctcaagct caagcgcggc gagatcgcac tggtgctgaa
     2281 cggcgcccgg gttgaggtcg ttgagatcga gtagcggcta aggcgccggt tgccagagct
     2341 cgacctgatt gccttcgggg tcttgaaggc gcgcgaagcg gccaacctca gggctgtccc
     2401 attcggcctt ggtgatgatc tcgacgccag ccgcccgcag gctggccatc atgccgtcga
     2461 ggtcgtcgac gcgcaggttc agcatccact gcttctcagc cgggaaatag tcggtgtcgg
     2521 ccttgaacgg cgagaacacc gtcggcccgg cctgctggtt ccagacgtac tggccctcag
     2581 gtgcgccgac gccgaggtgt tccagatacc atgcgccgag cgccttgggg tccttcgacc
     2641 tgaagaacaa tccgccaatg ccagtgaccg ccatgcccgt ctccccgatg ccgggagagc
     2701 atgggccgcc aaaggtcaca tatccagctt gagcttcatc acttcggcta gcgccttggc
     2761 gcggcggcgg gtctgttcgc cgagcagggc gtcggcgtag ccttcggaag cggtcagttt
     2821 cagcacgccc ttgtccaccc tgaccgtagc gttggccaac agctggggtg agcgaccgga
     2881 caggtcacag ccgagtcgca tggccagacc gagcgcgcgg gcgcggaggc gggtggtttc
     2941 ggtcaagagg cgctcgacga cggcaggatc cggcgtcgcc accgagccgc cgtaacgggc
     3001 gttgagcacg caggcgagga aggtgcgctc ggcgtgggtc tggcccggga tcggcgcgcg
     3061 caaaacctga tcgaagacca gttccagacg gtggtcggga tgcagacgag cgcccaggtc
     3121 ggccagttgg caggcggcgg agacgagcac ggcgtcgcgg gtcgatccaa aggcggtcgg
     3181 cagggccgcg atgacgtttt ccaaccagcc acgcaaggct ccgggcaggg tgggcgagat
     3241 gccctggcgc ccccccaagg cggcgcaacc ggcgatcagc gggtcttcgt cagcctcgtc
     3301 gcgatcgagc gcctcgaaca gcaggccctc gcgcagaccc caggccgaga aattgatcgt
     3361 cttgatgccc agagtgtcga tcagcccctc aagcacgagg gcggagtagg gcagggtctc
     3421 ggcgcgtttc tttgagacgc cgggcaggcg gtcgagcgag gcgcgcgact gctgggcgac
     3481 cagacgggcc acctcgcgcg cgtcgtcggc gttcatggcg tactggtgaa cgatgcggat
     3541 cggatagcgg ttcatggcca tgtgcagctg ggccagcgag cgccagcccc cgccgacggc
     3601 attcaggctg tcggtcgcga aggccttggc tgcgggtgta agcctggcag cgatggtgct
     3661 gcggaggcgg tcggcgtcga aacccgccgg gtcggcgagg ctgaatggac cgagcggcag
     3721 cgtgatgccg ggcttaagcg tcttgccctc aagccggatc aactcgaggc tggagccgcc
     3781 catgtcggct acgacgccct cggcagtcgg atcgccggcc agcacgccga gcgcggaata
     3841 acgggcctcc tcttcacccg tcaggacgcg gacctgaagc ccggtctcgg cggcgacgcg
     3901 gctgcagaag tctggtccgt ccagcgcctc gcgcaccgca gcggtggcgg cgacgaagac
     3961 ataggcgggg cgaacgcttt cgaggacgac cgcgaaccgc ctcagcgccg tcagggccat
     4021 ctcgatgcca ttcggggaca acttgccggt agcaggcagg tcgcggccca gacccgccag
     4081 aaccttttcg ttgaacacgg tccagatggc ccgcccttcc agccggtaga gcaccagacg
     4141 gacggagttg gagccgatgt cgacgacagc cgcttcgcgc ggcgtgttcc agggttcgtt
     4201 cacaggcggg gg
//
LOCUS       DASVTM010000521         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_473828,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000521 DASVTM010000000
VERSION     DASVTM010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473828"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..404)
                     /locus_tag="VF633_14275"
     CDS             complement(135..404)
                     /locus_tag="VF633_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX8472274.1"
                     /translation="MADPHSSNDAADHDAEAYQRGTMAINEQTATWSLVQKLFSWGSL
                     AIAAVLLALVMAFRPGGSIFVGFVCGLIVFIAGFFFLKSGSKKAH"
     gene            complement(495..>1529)
                     /locus_tag="VF633_14280"
     CDS             complement(495..>1529)
                     /locus_tag="VF633_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX8472275.1"
                     /translation="PADSRHLGNEVHGASVDAMAEAVAAAYPRLSHRYYALKARAMGK
                     PTLDQWDRNAPLENRAPRSFDWAQGRAMVLDSFADLGPEFAERAQWFFDRPWIDGRAR
                     PGKQSGAYAHPVTANRHPYVFLNWMGERRDVLTLAHELGHGIHQTLAADRGTLLADTP
                     LTLAETASIFAEGLSFDRLLATAPKAEQRGLLAGRIEDGLNTVVRQIAFHRFETRFHD
                     ERASGEVPAERIGQIWLEEMGASLGPAVTLNPGYEHWWSYVSHFVHSPFYVYAYAFGD
                     LLVAALMETRRKDPVAFTPLYRELLAGGGTRTYDEALKPFGLNPRDPAFWTIGCQRLE
                     RLVDQFEQTF"
BASE COUNT          277 a          481 c          532 g          239 t
ORIGIN      
        1 tggaatggat tggcgcgtca aagcgctaga gcggcttcct gggacgaaat gcgtgcgcga
       61 aacagggatt gaccggcaag ccatgccgca gcgggctggt cggtgcgcac ggcagagaac
      121 tgcggcgcga agacctagtg cgccttcttg gagcccgact tcaggaaaaa gaagcccgcg
      181 ataaagacga taagcccgca gacgaagcct acgaaaatgc tgccgcccgg acggaaggcc
      241 atgaccagag ccagcagcac cgcagcgatc gccagtgagc cccagctgaa cagcttctgg
      301 accaaagacc aggtcgccgt ctgttcgttg atcgccatcg tgccgcgctg atacgcctcg
      361 gcgtcatggt cggcggcgtc gttcgaggag tgcgggtcgg ccataggtga tcgtccggaa
      421 gagtcgatgg ttggcgagac ttatagcgag gtcgccgtca ggctcaagct acagtctcgc
      481 cacgaccgaa aggttcaaaa cgtctgttcg aactgatcga ccagccgttc cagcctctga
      541 caaccgatgg tccagaaggc gggatcgcgg gggttgaggc cgaacggttt gagcgcctca
      601 tcgtaggtcc gcgtgccgcc gccggccaga agctcgcggt acaggggcgt gaaggcgacc
      661 ggatccttcc tgcgcgtctc catcaacgcg gccaccagca ggtctccgaa ggcataggca
      721 tagacgtaga agggcgagtg gacgaagtgg ctgacgtagc tccaccagtg ttcgtagccg
      781 gggttcaggg tcaccgcggg gccgagcgac gcgcccattt cctcgagcca gatctggccg
      841 atgcgctcag cggggacctc gcccgaggcg cgctcgtcgt ggaagcgggt ctcgaagcgg
      901 tggaaggcga tctggcgcac gacggtgttc agtccgtcct cgatccgtcc ggccagcagg
      961 ccgcgttgct cggccttcgg agcggtggcc agcagccggt cgaagctcag gccctccgcg
     1021 aagatcgacg ctgtctcggc cagcgtcagc ggcgtgtcgg ccagcagcgt gccccgatcc
     1081 gcagcgagcg tctggtggat gccgtgaccc aactcatggg cgagggtaag cacatcgcgc
     1141 cgctcgccca tccagttcag gaagacatag gggtggcggt tggccgtgac cgggtgggcg
     1201 taggcgcccg actgcttgcc gggccgcgcc cgaccgtcga tccaaggccg gtcgaagaac
     1261 cattgcgcgc gctcggcgaa ctcgggaccg aggtcggcga aactgtccag caccatcgcc
     1321 cggccctgcg cccagtcgaa cgaccggggc gcgcggtttt ccagcggggc gttgcggtcc
     1381 cactgatcaa gcgtcggctt gcccatcgct ctggccttca gcgcatagta gcggtgcgac
     1441 agccggggat aggcggcggc gaccgcctcg gccatcgcat cgaccgaggc gccgtgcact
     1501 tcgttgccca ggtgccggct gtcggcagg
//
LOCUS       DASVTM010000522         5278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_153930,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000522 DASVTM010000000
VERSION     DASVTM010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153930"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..638
                     /locus_tag="VF633_14285"
     CDS             87..638
                     /locus_tag="VF633_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8472276.1"
                     /translation="MGPTIETDRLILRPTASSDFERWCDFQSDAATMRFIGGVKTPAE
                     TWRVMMTCAGAWALTGTSFFSVIEKASGQWMGRIGPWFPHQWPGTEVGWSLHRDAMGK
                     GYALEAAVAAMDYAVDVLGWDDVIHTIDPANIASAKLAARLGSTNRGPGRLPPPFEGS
                     RVDIWGQTAVEWRENRKAFDSFG"
     gene            670..1113
                     /gene="mmcB"
                     /locus_tag="VF633_14290"
     CDS             670..1113
                     /gene="mmcB"
                     /locus_tag="VF633_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair putative endonuclease MmcB"
                     /protein_id="HEX8472277.1"
                     /translation="MSAAFDLELVFSRPETTLSVTRGAARLLIDMGYAPLLEVCLPNG
                     RRADVMAVGKKGDIVICEVKSGVDDYRVDRKWPEYAPFCDAFYFAVAPEFPEGVLPEE
                     PGLIIADAFGGAVVRDAPVLALAPARRKALTLAFARLGAMRLLRD"
     gene            complement(1229..1786)
                     /locus_tag="VF633_14295"
     CDS             complement(1229..1786)
                     /locus_tag="VF633_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEX8472278.1"
                     /translation="MSVIEDRVAALHDKTLLLLDDDQALRTRLGRALESRGFTVTTAG
                     SVAEATDVLKTVVPAYAVLDMRLDDGNGLKIVEAIRERREDARIVMLTGYGAIATAVA
                     AVKAGAVDYLSKPADADDVVKALLAIGDASPEPPENPMSADRVRWEHIQRVYELCDHN
                     VSETARRLGMHRRTLQRILAKRAPR"
     gene            complement(1879..3333)
                     /locus_tag="VF633_14300"
     CDS             complement(1879..3333)
                     /locus_tag="VF633_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEX8472279.1"
                     /translation="MTSQPPLLGGEGPADHWRDPPRRGRGLTLRTLIILRWLTVLGQG
                     ATLWVAHKMLHFPLPLWPCIGVIAVSVAVNVGSLVWLRRTDNRLPDGRQTAIHLGFDI
                     LQLSTLLGLTGGLENPFCLLLVAPVTVAAASLPGRQAIGLGFLVLIATGVLFKWSLPL
                     PWQSGTELILPFLYRVGLGMALVTGVIFTSAYAWRVAADAEKLELALATTQDVLQREQ
                     RLAALGGLAAAAAHELGTPLATIQVVAKELLRGAPADSAVAEDAALLLQQADRCREIL
                     QRLSQRPEDGDALYAEIGLKTLLNEVVDPHLGFDLDIEVDVIGPVDEPAIQVKRLAEV
                     VHGLSAIVENAADFAGSRVRIRAIVDLDWIRIEVLDDGPGFAPDILPRLGEPYVTSRP
                     HGKARRALAAQIRAATRPPKAGLGRRKDSQPLLPPAETVIPSQGGMGLGFFIARTLLE
                     RTGGHVAVGAGDGPPGHRGARVTVRWPRSALEIT"
     gene            3419..4036
                     /locus_tag="VF633_14305"
     CDS             3419..4036
                     /locus_tag="VF633_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX8472280.1"
                     /translation="MPRRSILLFAAACLVIAISVATVTVVVVTGRDRGVTEQTSTGQP
                     AVGGPFQLVNQNGQTVDQTMLNGKWTLVFFGFTYCPDYCPTTLTGLAATKARMGDKAR
                     DLQIAFVSVDPERDTPAALKDYLSSDGFPEGVVGLTGTPDQIATVAREYRAFYKKVGE
                     GEGYTMNHTLTVYLMGPDGKFRAPLAYDLGPEKSAQLIQRVMARG"
     gene            4189..>5278
                     /gene="phaZ"
                     /locus_tag="VF633_14310"
     CDS             4189..>5278
                     /gene="phaZ"
                     /locus_tag="VF633_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX8472281.1"
                     /translation="MLYALHELAYQSAQPFRIGAQFARKFWTSPLNPASETAIGRTAY
                     ASAELFESVTRRYGKPDWGLEVITIDGKPVRTVEQVIWQSPWVRLVRFARNVGDLKRA
                     KKPTAAPSVLIVAPLSGHYATLLRGTVETFLQDHDVYVSDWTNARQVPMLEGRFDFHD
                     YIDHVRQMLAQIGPRAHVVAVCQPGPPVLAAAAVMAEENDPNRPASMTFMGSPIDARL
                     SPTVTNQLAEEKPFAWFKSKMIHTVPWPYPGFGRRVYPGFVQLYSFMSMNEERHTDAH
                     WTYFKDLVKGDGDGVEKHEHFYDEYLSVLDLSEEFYLQTIDIVFQRHLLPQGLLEHRG
                     RPVRPERITDVALMTVEGEKDDISGVGQT"
BASE COUNT          899 a         1692 c         1791 g          896 t
ORIGIN      
        1 ggtgaccatc gtgccaggag aactgaccga cagcgagatc gaggatcttg catgagtgcg
       61 tttgttcagg cgcccattct tgcgagttgg ggcctacgat cgagaccgac cggctgatcc
      121 tgcgtccgac ggcatcgagc gatttcgagc gctggtgcga cttccagtcc gacgccgcga
      181 cgatgcggtt catcggcggg gtcaagactc cggcggagac ctggcgcgtc atgatgacct
      241 gcgccggggc gtgggcattg acggggacca gcttcttttc ggtgatcgag aaggccagcg
      301 gtcagtggat gggccggatc ggtccgtggt ttccgcacca gtggcccgga accgaggtcg
      361 gctggagcct gcaccgcgac gccatgggta agggctatgc tctggaggcg gccgtggccg
      421 cgatggacta tgcggtcgat gtactcggct gggacgatgt catccacacc atcgaccccg
      481 cgaacatcgc ctcagccaaa ctggcggcgc ggctgggttc gaccaaccgg ggcccggggc
      541 gattgccacc gccgttcgaa ggctcgcgcg tcgatatctg gggtcagacg gctgtggaat
      601 ggcgtgagaa ccgcaaggcg ttcgacagct tcggttgaaa ccggaacgga cccggaacat
      661 aatggcggaa tgtccgccgc cttcgatctt gaactcgtct tcagccgccc tgagactacg
      721 ctgtcggtga cgcgcggcgc cgcgcgtttg ctgatcgaca tgggctatgc gccgctgctg
      781 gaggtctgcc tgccgaacgg tcgtcgggcc gacgtcatgg cggtgggcaa gaagggcgac
      841 atcgtcatct gcgaggtcaa gtcgggtgtc gacgactatc gcgtggatcg caaatggccc
      901 gaatatgctc cgttttgcga cgccttctac ttcgccgtcg cgccagagtt ccccgaaggc
      961 gtcctgcccg aagaacccgg cctgatcatc gccgacgcct tcggcggagc ggtggtccgc
     1021 gatgcgccgg tgctggcctt ggcgccggcg cggagaaagg cgctgacgct ggcttttgcg
     1081 cggctcgggg cgatgaggtt gctgagggac tagggctgac tattttgctg ttcggactgg
     1141 gtccccgctt tcgcggggat gagcggaaaa tcaaaaacgc caagccctaa gccctaagcc
     1201 ctaagcccta gccctaagcc cttgcgcgtc agcgcggagc ccgctttgcc aatatccgct
     1261 gcagggtccg tctgtgcatg cccagtcgcc gggcggtttc cgagacgttg tggtcgcaga
     1321 gttcgtagac gcgctggatg tgttcccagc ggacgcggtc ggcgctcatg gggttttcgg
     1381 gcggctcggg cgaggcgtcg ccgattgcca gcagggcctt gaccacatcg tcggcgtcgg
     1441 cgggcttgga caggtagtcg accgcgccgg ccttgaccgc cgcaacggcg gtggcgatgg
     1501 cgccatagcc ggtcagcatc acgatgcggg cgtcctcgcg gcgttcgcgg atggcctcca
     1561 cgatcttgag gccgttgccg tcgtccagac gcatgtcgag cacggcgtag gccggcacca
     1621 cagtcttgag cacgtcggtg gcctcggcga ccgatccggc tgtcgtgact gtaaagccgc
     1681 gcgactccag cgcccgaccc aaccgggtgc gcagcgcctg atcgtcgtcg agcagcaaca
     1741 gggttttatc gtgaagcgcg gcgacgcgat cctcgatgac tgacatttcc atggtctcca
     1801 tgagcctcaa gtcgcggtcc ggacccccgg ggtcaagtct gacgcggcga ttggtcgctg
     1861 gtcaggaagc gcctgtaatc aagtgatttc aagcgcggat cgcggccagc ggacggtcac
     1921 gcgcgccccg cgatggccgg gagggccgtc gccagcgccg accgcgacat ggccgccggt
     1981 ccgctccagc agggtgcggg cgatgaagaa gccgaggccc atgccgccct gactggggat
     2041 cacggtctcg gccgggggca ggagcggctg ggagtccttg cggcggccca agccggcctt
     2101 gggaggacgg gtcgcggcgc ggatctgggc ggcgagagcg cgtcgggctt ttccgtgcgg
     2161 acggctggtg acatagggct cgccgagacg gggcaagatg tccggggcga aaccggggcc
     2221 gtcatccagc acctcgatcc ggatccagtc gagatcgact atcgcccgga tgcggacccg
     2281 ggagccggcg aaatccgctg cgttctccac gatggcgctc aggccgtgaa ccacttccgc
     2341 cagtcgcttg acctgtatcg cgggctcgtc gaccggaccg atcacatcga cttcgatgtc
     2401 gaggtcgaag ccgaggtgcg gatcgacgac ctcattgagc aacgtcttga gcccgatctc
     2461 ggcatagaga gcgtcgccgt cttccgggcg ctgggacagg cgctgcagga tttcgcggca
     2521 gcggtcagcc tgttgcagca gcagggcggc gtcctcggcc acggcgctgt cggccggagc
     2581 gccccgcaac aactccttgg ccacgacctg gatggtagcc agaggcgtgc ccagttcgtg
     2641 ggcggcggcg gcggcgaggc ccccaagagc cgccagccgc tgctcacgct gaaggacgtc
     2701 ctgggtcgta gccagcgcca gttccagctt ctcggcgtcc gctgcgaccc gccaggcata
     2761 ggccgaggtg aagatcaccc cggtcaccag agccatgcca aggccgacgc ggtagaggaa
     2821 aggcaggatt agctcggtgc ccgactgcca gggcaggggc agcgaccatt tgaacagcac
     2881 gcccgtcgcg atcagcacca gaaaaccgag acctatggcc tgacgacccg gaagtgacgc
     2941 tgccgccacc gtcaccgggg cgaccagcag caggcagaac gggttctcca gcccgccggt
     3001 caggccaagc agggtcgaga gctggaggat gtcgaaaccc aggtggatcg ccgtctggcg
     3061 gccgtccggc aggcggttat cggtccgtct cagccacacc agcgagccga cgtttaccgc
     3121 gacgctgacc gcgataaccc cgatgcaggg ccacagtggt agcgggaaat gcagcatctt
     3181 atgcgcgacc cacagcgtgg ctccctgtcc cagaaccgtc agccagcgca ggatgatcag
     3241 ggtgcgaagg gtcaggccgc gtccacgccg tggcggatcg cgccagtgat cggcaggccc
     3301 ctctccaccg agaagcgggg gctgcgacgt catgccgggc gaagaggcgg tttcacggat
     3361 gtccacgacc gttctataga ccggacgccg acgcctgtcg gcagttcggg aagaccaaat
     3421 gccacgccgt tccatattgc tgttcgcggc cgcctgtctc gtcatcgcca tctcggtggc
     3481 cacggtgacc gtggtcgtcg tcaccggtcg cgatcgcggc gtgaccgagc agacctcgac
     3541 gggtcagcct gcggtcgggg gtcccttcca gctggtcaat cagaacggcc agacggtcga
     3601 ccagacgatg ctgaacggca aatggacgct ggtcttcttc ggcttcacct actgccccga
     3661 ctattgcccc acgaccctga ccgggctggc ggcgaccaag gcgcggatgg gcgacaaggc
     3721 cagggatctg cagatcgcct tcgtcagcgt cgatcccgag cgcgacacgc ctgcggccct
     3781 gaaggactat ctgtcgtcgg atggtttccc agagggcgtc gtcggcctga cgggcacgcc
     3841 cgaccagatt gccaccgtcg cccgcgaata tcgcgccttc tacaagaagg tcggcgaagg
     3901 cgagggctac acgatgaacc acaccctgac ggtttacctg atgggccccg acggcaagtt
     3961 ccgtgcgcca ttggcgtatg acttggggcc agagaagtcg gcgcagctga tccagcgggt
     4021 gatggctaga ggataggccg gagaggcagt aggcaggtag gcaataggca ttaggtaggt
     4081 cggtctgatc ccgacggcat accgtcgcgc gcttctgcct attgcctacc tgcctactgc
     4141 ctcctctaat cccttgcgcc ctccccagca caggccgata tcttctccat gctctacgca
     4201 cttcacgagc tcgcctatca atcggcgcag ccattccgga tcggggcgca gttcgcgcgc
     4261 aaattctgga cgtcaccgct caatccggcg tcggagaccg cgatcggtcg gactgcctat
     4321 gcctcggccg agttgttcga gagcgtgacg cgccgctacg gcaagcccga ctgggggctt
     4381 gaggtcatca ccatcgacgg caagccggtg cggacggtcg agcaggtcat ctggcaaagt
     4441 ccctgggttc gtctggtgcg gttcgcgcgc aatgtcggcg atctgaagcg ggcgaagaag
     4501 ccgacggcgg cgcccagcgt cctgatcgtc gcgcctctgt cgggccacta tgcgaccctg
     4561 ctgcggggca cggtcgagac cttccttcag gaccacgacg tctacgtctc ggactggacc
     4621 aacgcgcggc aggtgccgat gctggagggc cggttcgact tccacgatta tatcgaccac
     4681 gtgcgccaga tgctggccca gatcggcccg cgcgcccacg tcgtcgccgt ctgccaaccg
     4741 gggccgcccg tgctcgccgc cgccgctgtg atggctgagg agaacgatcc caaccgcccc
     4801 gcgtcgatga ccttcatggg ctcgccgatc gatgcgcgcc tgtcgccgac cgtgaccaac
     4861 cagctggcgg aggaaaagcc cttcgcctgg ttcaaatcga agatgatcca caccgtgccc
     4921 tggccgtatc cggggttcgg gcgccgggtc tatccgggct tcgtccagct ctacagcttc
     4981 atgtcgatga acgaggagcg gcacaccgac gcccactgga cctatttcaa ggatctggtg
     5041 aagggcgacg gcgacggggt cgagaagcac gaacatttct acgacgagta tctgtcggtg
     5101 ctggatctgt cggaggagtt ctatctccag accatcgaca tcgtgttcca gcgccacctg
     5161 ctgccgcagg gcctgctcga gcaccgcggc cgcccggtgc ggccggagcg gatcaccgat
     5221 gtggcgctga tgaccgtcga gggcgagaag gacgacatct ccggcgtcgg ccagacgc
//
LOCUS       DASVTM010000523         5514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_531000,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000523 DASVTM010000000
VERSION     DASVTM010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531000"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF633_14315"
     CDS             complement(<1..293)
                     /locus_tag="VF633_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472282.1"
                     /translation="MFRGVCVAPEQFVGQGDLMVAMNVDPARVHEFTDIAAFHDWLSQ
                     NHEREPEVWIKIHKVGSGRASITTKEAIDVALCWGWIDAIRKSFDERSFLQRYT"
     gene            complement(305..2239)
                     /gene="ftsH"
                     /locus_tag="VF633_14320"
     CDS             complement(305..2239)
                     /gene="ftsH"
                     /locus_tag="VF633_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8472283.1"
                     /translation="MNLRNLAIWGLILLGLVAIYAAMSQNGGPLATTKGGAAAASQQK
                     IITYSELVSAVNAKSVKAVVVKGDQIQGEFQNGSRFVSTTLQQNEPLIEMIRQSGAAV
                     DVKTTRQSIWMSALLGLLPILLLVGVWIFFMRQMQGGARGAMGFGKSKAKLLTEHKGR
                     KMFEDVAGVDEAKEELQEVVDFLKDPAKFQRLGGKIPKGALLVGPPGTGKTLLARAVA
                     GEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGL
                     GGGNDEREQTLNQLLVEMDGFESNEGIILIAATNRPDVLDPALLRPGRFDRQVVVPNP
                     DVNGREKILRVHMKDVPLASDVNVKTIARGTPGFSGADLANLVNEAALMAARKDRRMV
                     THRDFEDAKDKVMMGAERKSMAMNEEERRLTAYHEGGHAIVAINVKMADPVHKATIVP
                     RGQALGMVMQLPEGDRYSMKYQQMVDRIAIMAGGRVAEEIIFGKQNITSGASSDIQQA
                     TKLARRMVTQWGFSEILGTVAYGENEQEVFLGHSVARSQNISEETARIIDSEVKRLVT
                     EGWNEARQILTDKAEDLEKLAQALLEYETLSGIEIKDLLEKGAAPNRDENNFPNSGPS
                     VSVPVTPVTEPVAIEAPTVH"
     gene            complement(2268..3554)
                     /gene="tilS"
                     /locus_tag="VF633_14325"
     CDS             complement(2268..3554)
                     /gene="tilS"
                     /locus_tag="VF633_14325"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX8472284.1"
                     /translation="MRDAGHPVVLGLSGGGDSMALLEMAAGWTRSRGRRLLAVTVDHG
                     LNPASAEWSRTCADASIALGVDWIEARWVGDKPATGLTAAARTARHGLIADVARQTGA
                     KVVLLAHTADDIAESDWMRTRGSNLGRLREWSPSPVWPEGRGLMLFRPLLDERREDLR
                     NLLRARGKSWIEDPANADFRFGRSQARHSLSDLTKGYVGSTERAPDPACLFPAGALGV
                     WPRALPLGEGFEVPRGVFPSALAATLLSASGSATPPRHADLSRLNARLRSGEAFTATL
                     SGARIDASIDRILIGREPGEFRRSPPTDLTLRPDEPMVWDARYEISAFEPGWTVTSTA
                     GRLSRLSEVDRAVIATIPAWARGALPVLLRDGESAPILAWRKAEVRALAPRRLALFLG
                     AVSGETTQEANLFQSIHGETPPADLFSHNDHHRGLG"
     gene            complement(3611..4453)
                     /locus_tag="VF633_14330"
     CDS             complement(3611..4453)
                     /locus_tag="VF633_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein"
                     /protein_id="HEX8472285.1"
                     /translation="MKLKPLHRNVLLASVGICLVGGIAVSQTQPLAPVQWDNRRLDTL
                     DRNVRRLERALTQRNAVGQPVLVEPDPETVALQGQVGDLDRRLQDLESTLQRMTRDQE
                     QTAFKLDEVTRDNAALLTRLGETETRIKASEDRAQAAAAAAAEAEQAGRSPTGDANAD
                     LAAARALPDPAAQAQAYETLITNWPSTPQAGEAGYRLGDLKRGGNDMAGAVQAYAGAL
                     AGWPTTPWAGETTLKLARALAATDRNTQACGALGEFNRRYGAAASAQLKTIAGQIRTQ
                     ARCT"
     gene            complement(4567..5121)
                     /gene="pal"
                     /locus_tag="VF633_14335"
     CDS             complement(4567..5121)
                     /gene="pal"
                     /locus_tag="VF633_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX8472286.1"
                     /translation="MTTINTVIKLAAVSLVMASMAACTPKRAVQPVVPNEPAGPSNPV
                     YPGAGTGNVGGGDMNAARPGSEQDFVVNVGDRVYFDLDSYQVSREAYPRLDAQAQWLL
                     RYPQITVRIEGNADERGTREYNLALGARRAESVRNYLVQRGVPAGRIDTISFGKERPI
                     AEGSSEESWARNRNGHTAIVSGGM"
     gene            complement(5286..>5514)
                     /gene="tolB"
                     /locus_tag="VF633_14340"
     CDS             complement(5286..>5514)
                     /gene="tolB"
                     /locus_tag="VF633_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268280.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEX8472287.1"
                     /translation="SYFEEGPSWAPNGRYIMFARQTRGGNTNLWMVDLSGRVIAQSGY
                     QGRGSDPAWSPLLDAAPVSLGRNQGPDSCPS"
BASE COUNT          933 a         1726 c         1823 g         1032 t
ORIGIN      
        1 gtatagcgct gcaggaaact tctctcgtcg aagctcttcc tgatcgcatc gatccagccc
       61 cagcacaggg cgacgtcgat cgcctctttc gtcgtgatag aggcccggcc ggagccgacc
      121 ttgtggattt tgatccagac ctcgggctca cgttcgtgat tttgggagag ccagtcgtga
      181 aaggcggcga tgtccgtgaa ctcatgaacc ctggcgggat caacgttcat cgcaaccatt
      241 aagtccccct gtccgacgaa ttgttccgga gcgacgcaga cgccccggaa catctatcag
      301 acttctagtg aaccgtcgga gcttcgatgg cgacaggttc cgtaactggc gtaaccggca
      361 cagaaaccga ggggccggag ttggggaagt tgttttcgtc ccggttcggc gcggcgccct
      421 tctccagcaa gtccttgatc tcgatacctg acaaggtttc gtattcgagc agggcctgtg
      481 ccagcttctc gaggtcttcg gccttgtcgg tcagtatctg gcgggcttcg ttccagcctt
      541 cggtgaccag acgcttgacc tcggaatcga tgatccgggc cgtctcttca ctgatgttct
      601 ggctgcgcgc gaccgagtgg ccgaggaaca cttcctgctc gttctcgcca taagcgacgg
      661 tgccgaggat ttccgagaag ccccactggg tgaccattcg gcgggccagc ttggtcgcct
      721 gctggatatc cgacgacgcg cctgaagtaa tattttgctt gccgaagatg atctcctcgg
      781 cgacgcgacc accggccatg atggcgatgc gatcgaccat ctgctggtac ttcatcgaat
      841 agcggtcgcc ttcaggcaac tgcatgacca tgcccagggc ctgaccgcgc gggacgatcg
      901 tcgccttgtg gaccggatcg gccatcttga cgttgatggc gacgatggcg tggccgccct
      961 cgtgataggc ggtcaggcga cgctcttcct cgttcatggc catggatttg cgctcggcgc
     1021 ccatcatgac cttgtccttg gcgtcctcga agtcgcggtg ggtaaccatg cgccggtcct
     1081 tgcgcgcggc catcagcgcc gcttcgttga ccaggttggc aaggtcggcg ccggagaagc
     1141 ccggggtgcc gcgcgcaatg gtcttgacgt tgacgtctga ggccagcgga acgtccttca
     1201 tgtggacgcg caggatcttt tcgcggccgt tcacgtcggg attcggcacc acgacctgac
     1261 ggtcgaaacg gcccggacga agcagggcgg ggtccagcac gtcgggacgg ttggtggcgg
     1321 cgatcaggat gatgccttcg ttggactcga agccgtccat ctcgaccagc agctggttca
     1381 gcgtctgctc gcgctcgtca ttgccaccgc cgaggcctgc gccacggtga cgaccgacgg
     1441 cgtcgatttc atcgatgaag atgatgcagg gggcgttctt cttggcctgt tcgaacatgt
     1501 cgcgaacccg cgacgcgccg acgccgacga acatctcgac gaaatccgag ccggagatcg
     1561 agaagaaggg cacgccggcc tcgcctgcaa cggcgcgggc cagcagggtc ttacccgtac
     1621 cgggagggcc cacgagcagg gcgcccttgg ggattttgcc accgaggcgc tggaacttgg
     1681 ccgggtcctt gaggaagtcg acgacttcct gaagttcttc cttggcttcg tcgacgccgg
     1741 cgacgtcctc gaacatcttg cgacccttgt gctcggtcag cagcttggcc ttggacttgc
     1801 cgaagcccat ggctccgcgc gcgccgccct gcatctggcg catgaagaaa atccagacac
     1861 caaccagcag cagaatgggg aggagcccaa gcagggcgct catccagatc gattgacggg
     1921 tggtcttcac gtcgaccgcc gcgcctgact ggcggatcat ctcgatcagg ggttcattct
     1981 gctgcaaggt cgtcgaaacg aaacgcgagc cgttctggaa ctcgccctgg atctggtcgc
     2041 ccttgacgac cacggccttg acgctcttcg cgttaacggc gctcacgagt tcggaatagg
     2101 tgatgatctt ctgctggctc gcggcggcgg cgccgccttt ggtcgtcgcg aggggaccac
     2161 cgttctggct catggcggcg tagatcgcca ccaggcccag caggatcaag ccccatatcg
     2221 ccaggttacg caggttcatt atgcttctca gtccttgggc aaggcgatca gcctaggccg
     2281 cgatggtggt cattgtgtga aaataggtca gcgggcggcg tttcgccatg gatggattga
     2341 aacaggttcg cctcctgcgt cgtttcgccc gatactgctc ccaggaaaag cgccagacgt
     2401 cgtggagcca aggcccgaac ctcggctttc cgccacgcca gaattggcgc ggattcaccg
     2461 tcccgcaaca agacaggcag ggctccgcgc gcccatgcgg gaatggtggc gatgacggcc
     2521 cgatcaacct ccgagagtct ggaaagtcgg cccgctgtgc tcgtcacggt ccacccgggt
     2581 tcgaacgccg agatttcgta acgggcgtcc cagaccatag gttcgtctgg cctgagggtc
     2641 aggtcggtgg gaggggatcg ccggaattct cccggttcgc gaccgatgag gatgcggtcg
     2701 atcgacgcgt cgatgcgagc tccggacaga gtggcggtga aggcctcgcc tgagcggagg
     2761 cgggcattca gccgggacag atcagcatga cgaggcggtg ttgctgatcc agacgcactg
     2821 agcagcgtag cggccaacgc actcggaaag acgccccggg ggacttcaaa cccctctccc
     2881 aaagggagag cgcgcggcca gacccccaag gcgccggcag ggaaaaggca ggcggggtct
     2941 ggcgcgcgtt ctgtcgatcc gacgtatccc ttcgtgagat cggaaagact gtgtcgcgcc
     3001 tgcgaacggc cgaagcgaaa atcggcattt gcgggatcct caatccagct tttgccgcgc
     3061 gcgcgaagca gattccgcag atcctcacgc cgttcgtcca gcagcggccg gaacagcatc
     3121 agccccctgc cctcgggcca gaccggagac ggcgaccatt cgcgcaggcg accaaggttg
     3181 gagccgcgcg tccgcatcca gtcgctttcg gcgatatcgt cggcggtgtg ggccagcagc
     3241 acgaccttgg cgccggtctg gcgcgccacg tctgcgatga ggccgtgccg ggccgtccgg
     3301 gctgctgccg tcaaaccagt cgcaggcttg tcgccaaccc agcgcgcttc gatccagtcg
     3361 acacccaaag cgatgcttgc gtcggcgcag gtccggctcc attccgcgct ggcagggttc
     3421 agcccgtgat cgacggtgac ggccaacaac cgacggccgc gcgatcgggt ccagcctgca
     3481 gccatctcga gtaaggccat cgagtccccg ccgccggaca agccgaggac gacggggtgg
     3541 ccggcgtctc gcatcagccg ggcgtcgaga cgcgcctcta gccgttcgct cagtccggcg
     3601 ggtccggcgt tcaggtgcag cgagcctggg tgcggatctg accggcgatg gtcttgagct
     3661 gagccgaagc cgccgcgcca tagcgcctgt tgaactcgcc gagtgcgcca caggcctgtg
     3721 tgttgcgatc cgtcgccgcc agagcgcgcg ccagcttcaa cgtcgtttca ccggcccagg
     3781 gcgtggtcgg ccagccggcc agcgcgccgg cataggcctg aacggcgccc gccatatcgt
     3841 tgccgcctcg cttgagatcg ccgagccgat aacccgcctc gcctgcctgg ggggtcgaag
     3901 gccagttggt gatcagggtc tcgtaagcct gagcctgtgc cgcaggatca ggcaaggcgc
     3961 gggcggcagc gaggtcagcg ttggcgtcgc ccgttggcga acggcccgct tgttcagcct
     4021 cggcagcggc ggctgcagca gcctgcgccc gatcttccga ggccttgatg cgggtctccg
     4081 tctcacccag acgcgtcagc agggcggcgt tgtcgcgcgt gacttcatcc agcttgaacg
     4141 ctgtctgttc ctgatcgcgc gtcatgcgct gcagggtcga ttcgaggtcc tgtagtcttc
     4201 ggtcgagatc gccgacctgt ccctgcaagg cgactgtctc aggatccggt tcaaccagaa
     4261 cgggctgacc gaccgcgttg cgctgggtca gggcgcgttc gaggcggcgg acgttgcggt
     4321 ccagcgtgtc gagacggcgg ttgtcccact ggaccggcgc cagcggctgg gtctgggaaa
     4381 ctgcgatgcc gccgaccagg cagatgccga cggaggcgag cagaacgttg cgatggagag
     4441 gcttgagctt catggcgcta caggtttcac cgtttcaggg ccttcgcaag gccagtccct
     4501 atggatcggg aacgaaaagg ggcgcccgca ttgcgagcgc cccgaatgtc ttctcgtcgc
     4561 cggggattac atcccgccgg aaacgatcgc ggtgtgaccg ttacggttgc gggcccacga
     4621 ctcttcgctc gaaccttcag cgatcgggcg ttccttgccg aagctgatgg tgtcgatgcg
     4681 gcccgcaggc acgccgcgct gcaccagata gttgcgcacc gactctgccc ggcgagcgcc
     4741 gagggcaagg ttgtactcgc gagtgccgcg ctcatctgcg ttgccctcga tacgcaccgt
     4801 gatttgggga taacgcagca gccattgggc ttgcgcgtcg agacgtggat aagcctcgcg
     4861 gctgacctga taggaatcaa ggtcgaaata gactcggtcg ccgacgttga cgacgaagtc
     4921 ctgttccgag cccggacggg cagcgttcat gtcgccgccg ccgacattgc cggttccagc
     4981 gccggggtag accgggttgg atggtcctgc cggctcgttc gggacgaccg gctggacggc
     5041 tcgctttggc gtacaggcgg ccatggaggc catcacgagg ctgacggcag ccagcttgat
     5101 cacggtgttg atggtcgtca tcgcggtcgt ctccttggtg ttgccctgga cgccctgtcg
     5161 gcgcttcaag cttagcctta gcggctcagg tggcggaatg tgggcgtttc aggctgtgat
     5221 aacgcacagt tgagacgtta ggctccgcat atgacgggtt tcgccagacg cggggtttct
     5281 gaaaatcacg agggacagct gtccgggccc tgattacggc ccaggctgac gggcgcggcg
     5341 tcgagcagcg gcgaccatgc ggggtccgag ccccggccct gatagcccga ctgggcgatg
     5401 acccgaccgg acagatcgac catccagagg ttggtgtttc cgccccgggt ctgacgcgcg
     5461 aacatgatgt agcggccatt cggcgcccac gaggggcctt cctcgaaata gctg
//
LOCUS       DASVTM010000524         7990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_154041,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000524 DASVTM010000000
VERSION     DASVTM010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154041"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF633_14345"
     CDS             <1..425
                     /locus_tag="VF633_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX8472288.1"
                     /translation="RGHIKIRAVDAGRGDELIENLLTAAPETFVPCMEAAIAATEAGP
                     ESLVARGEAWTKFRVPAVLASPLERALGVCWPWGDPSLGPQLKAEWVKAVNAGLGETG
                     VTMAVAPLGVLAEPGGVLDQLEAQGLEIDGPVWREDQR"
     gene            complement(430..999)
                     /locus_tag="VF633_14350"
     CDS             complement(430..999)
                     /locus_tag="VF633_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472289.1"
                     /translation="MTGKDILDYISQPKVRWVMAGVVVVAAVGLGGLALSGRDGGEAR
                     AGGGAELSVAVVAPVEPDVEPGGVMDVGELNNGFDGKLPEAPAARPEAGDYYAEQPAY
                     TENRYYSSAPRAPAPRPGDEAHLYMDQPVMDRDAMPVRRENPRAFGFDQPRPDYERER
                     EARRAAMDAREAARQARRERPDLGPDAFY"
     gene            complement(1102..2532)
                     /gene="tldD"
                     /locus_tag="VF633_14355"
     CDS             complement(1102..2532)
                     /gene="tldD"
                     /locus_tag="VF633_14355"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX8472290.1"
                     /translation="MTVHTPLPPILETAGVDPSEALSILQNALIGADDGELFLERSES
                     ESLVFDDGRLKSAAYDATEGFGLRVVAGETAGYAHANEISTASLRRAADSAAQAKRGH
                     AGVTAEGPRATNQKLYDEVDPLASPAFSDKIALLAEIDAWARARDPRVVQVSASIIGE
                     RRAIEILRADGLTVRDIRPLVRLNVSVTVERDGRRESASTGAGGRAGFDAWVVPARWQ
                     GQVDEAIRQALVNLDAVDCPAGEMDVVLGAGWPGVLLHEAIGHGFEGDFHRKGSSVFS
                     GMMGKRVAAPGVTVVDDGSIAGRRGSLSVDDEGTPTSRTVLIEDGIMVGLMNDRLSAR
                     QLGQRATGNGRRQSFAHMPMPRMTNTFMEGGSDKQADMIASTKRGLYAANFGGGQVDI
                     TNGKFVFQCTEAYLIEDGKITAPVRGATLIGDGATALTQIKMIGDDFAFDPGVGVCGK
                     AGQGVPVGIGQPSIKIGGLTVGGTAV"
     gene            complement(2629..3417)
                     /locus_tag="VF633_14360"
     CDS             complement(2629..3417)
                     /locus_tag="VF633_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX8472291.1"
                     /translation="MIRTLLIIAGAAFVLALASLAGGVAVGGRDMARHGWEWTFPSHG
                     EGGDLHIVREDGTEPAEVTRTLAWAGGDKLVVDLDAADVEYVQGDTPGITVRGRADRI
                     DNVRIDGDRLSWAGDESNKDERLVFGRHANRAGFWVDSGDIHVVVTSPAVKTFEINGS
                     ADVAIKGYDQPSLTIASTGSGDVSASGATKALALTMTGSGDADLSDLLTEDAEVDTSG
                     SGDATIAPTGAAVINLSGSGDLDLATRPSTLRQSISGSGDVRQD"
     gene            complement(3414..4019)
                     /locus_tag="VF633_14365"
     CDS             complement(3414..4019)
                     /locus_tag="VF633_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEX8472292.1"
                     /translation="MTRADFLARLKRGLVGLPTSTATEILNDYEAHFDDGKAAGRSEA
                     EVADALGNPDRLARELKAEAGVQRWTQEQTPSAAAGAVFAVLGLGALDIIILLPILMG
                     VIGTLFGILVAVIAMFVSGGAVMVAGPFAGFPGGGLAAFFVGLGLMAGAVAAGALLAI
                     LTILLVNGLIWFARLHYRLLKPALEPHAAPTAATSTSGDPV"
     gene            complement(4016..4384)
                     /locus_tag="VF633_14370"
     CDS             complement(4016..4384)
                     /locus_tag="VF633_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8472293.1"
                     /translation="MPETIEIQLKKGVLALCVLALLKRGDSYAYEIASRLSDAIDMGE
                     GTIYPLMRRMQSDGLVETYLVESSAGPSRKYYRLSEAGRSALAVQTADWKTFTDAVDA
                     VLNETAPAPTLETVATGDVK"
     gene            4689..5702
                     /gene="coxB"
                     /locus_tag="VF633_14375"
     CDS             4689..5702
                     /gene="coxB"
                     /locus_tag="VF633_14375"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8472294.1"
                     /translation="MASAAFAVLTASPVWAQDRLGQPTPGGIDLQPAASVLKHDAIWF
                     HNAILLPMCIGISLLVLGLIVWIAIRYNKKSNPVPARWSHNTTIEIIWTILPVMILVG
                     ISLFSFRLLFAYHDMPTPDLTIKATGNQWNWAYEYPDQGIAEYVSNILPEEELAKRGM
                     PHDLYRLAADEPLVVPVGKVVRVLVTGADVIHNFALPAFGLKTDAVPGRVNETWFRAE
                     REGVFYGQCSELCGDDHAFMPIEIHVVSQAAFAQWVVSKGGSMTPKTAAEAPGGTTAQ
                     IAAGTTGAPVAPGVNMAAPATTDGPAAPVGATTGAPGNGPSTAIAPATTSAAAPAAPS
                     AAQ"
     gene            5724..7403
                     /gene="ctaD"
                     /locus_tag="VF633_14380"
     CDS             5724..7403
                     /gene="ctaD"
                     /locus_tag="VF633_14380"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX8472295.1"
                     /translation="MATDITADDHAAPGHDAHAEHDHKPGFFTRWFLSTNHKDIGTLY
                     LLFAIMAGIVGGALSGLIRWELAEPGIQLFKEGSWVQYLGIVDASKHGYNATVTAHAL
                     IMIFFMVMPAMIGGFGNWFVPIMIGAPDMAFPRMNNVSFWLLVAAWCLLIMSMFADGG
                     SGHGIGGGWTIYPPLSTKGSPGPAGDLAIFALHVAGASSILGAINFITTIFNMRAPGM
                     TLHRMPLFAWSVLITAFLLLLSLPVLAGAITMLLADRNFGTAFFDPAGGGDPVMFQHL
                     FWFFGHPEVYILILPGFGIISHIVSTFSRKPVFGYLAMAYAMVAIGFIGFIVWAHHMY
                     TVGMTVNLRAYFVAATMIIAVPTGVKIFSWIATMWGGSIDFKTPMLWAIGFIFLFTVG
                     GVTGVVLSNAGIDYSLHDTYYVVAHFHYVLSLGAVFAIFAGFYYWFEKMFGVKYNEFL
                     GATHFWIMFVGVNVVFFPQHFLGLQGMPRRYVDYPDAFAFWNLVSSIGYVITIVGVGV
                     FLVMLAEAAIRRRPGVANPWGEGATTLEWTLSSPPPHHQFNELPVVKEDAH"
     gene            7462..7857
                     /locus_tag="VF633_14385"
     CDS             7462..7857
                     /locus_tag="VF633_14385"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8472296.1"
                     /translation="MTECEPPPVFDRYEQRVRERLLNLRRLILDVAETERVGPLEEVL
                     KWGEPSFVTHQPRTGSTIRINAVGPSHYGLFVNCKTSLADTFRSLYPDDLTVIGDREL
                     RFGIDDELPEGPVRRCIALALTYHRWKPR"
BASE COUNT         1297 a         2662 c         2584 g         1447 t
ORIGIN      
        1 ggagaggtca catcaagatc cgggcggttg atgcggggcg gggcgatgaa ctgatcgaaa
       61 acctgctgac tgctgctccc gaaaccttcg tgccgtgcat ggaagccgcg atcgccgcga
      121 ccgaagctgg gccggagtcg ctcgttgccc gaggcgaggc ctggacgaaa ttccgcgtgc
      181 cggctgtgtt ggcctcgccg ctcgaacggg cgctcggcgt ctgttggccg tggggtgatc
      241 ccagccttgg accgcaactc aaggccgagt gggtgaaggc cgtcaacgca ggactcggcg
      301 agacgggcgt caccatggcg gtcgcgccgt taggcgtgct cgccgagccc ggcggcgtcc
      361 tcgaccagct tgaagcccag gggctggaga tcgacgggcc ggtctggcgc gaggaccagc
      421 ggtagacgtc tagtagaagg cgtcggggcc caggtcggga cgttcccggc gcgcctgacg
      481 tgccgcctcg cgggcgtcca tggcggcgcg ccgggcttcg cgttcacgct catagtccgg
      541 acgcggctga tcgaatccga aggcgcgggg gttttcgcgg cgaaccggca tggcgtcccg
      601 gtccattacg ggctgatcca tgtagaggtg agcctcatcg cccggccgag gcgcaggcgc
      661 tcgcggcgcg gacgaatagt accggttttc cgtataggcc ggctgttcgg cgtagtagtc
      721 accggcctcg ggccgggccg ccggagcctc cggcagcttg ccgtcgaaac cgttgttcag
      781 ctccccgaca tccatgaccc cgccgggctc gacgtcgggt tcgacgggag ccaccacggc
      841 gacgctgagt tcggcacctc cgcccgctcg ggcctcacca ccgtcgcgac cagacagggc
      901 cagtccgcca aggccgaccg cagccacgac cacgacgccg gccatgaccc agcgcacttt
      961 cggctggctg atgtagtcga gaatgtcctt gccggtcatc ggtcaaatcc ctgcacacgc
     1021 ccatccctca tgccggaacc cggtcatggg gtcgcggttc cggaaccctg atcgttcagt
     1081 ccttcgctag acggccattc atcagacggc tgtgccgccg acggtcagcc cgccgatctt
     1141 gatcgagggc tggccaatgc cgaccgggac gccctgtccg gccttgccgc agacgccgac
     1201 gcccggatcg aaagcgaaat cgtcgccgat catcttgatc tgggtcaggg ccgtagcgcc
     1261 gtcgccgatc agggtcgcgc ctcgcaccgg cgcggtgatc ttcccgtctt cgatcagata
     1321 ggcctcggtg cactggaaca cgaacttgcc gttggtgatg tccacctgac cgccgccaaa
     1381 attggccgcg tacaagcccc gcttggtcga ggcgatcatg tcggcctgtt tgtccgaacc
     1441 gccctccata aaggtgttgg tcatgcgcgg catgggcatg tgggcgaacg actggcgccg
     1501 gccgttgccc gtggcgcgct ggccgagttg gcgggctgac aaccggtcgt tcatcagccc
     1561 gaccatgatg ccgtcctcga tcaggaccgt gcgtgaggtc ggcgtgcctt cgtcatcgac
     1621 agacaacgat ccccgacgtc cggcgatgga gccgtcatcg accacggtca cacccggtgc
     1681 ggcgacgcgc ttgcccatca tgcccgagaa caccgacgat cccttgcggt ggaagtcgcc
     1741 ctcgaagccg tggccgatgg cttcgtgcag cagcaccccg ggccagccgg cgccgagcac
     1801 cacgtccatc tcacccgcgg ggcaatcgac agcgtccaga ttgaccagcg cctgccgtat
     1861 ggcctcatcg acctgacctt gccagcgagc aggtacgacc caggcgtcga agcctgcccg
     1921 tccgccggcg ccggtggacg cgctttcacg tcgcccgtcg cgttcgacgg tgaccgagac
     1981 gttcaggcgg accagcgggc ggatatcgcg tacggtcagg ccgtcggcgc gcaggatctc
     2041 gatggcccgg cgctcgccga tgatggaggc ggagacctga accacgcgcg ggtctcgcgc
     2101 ccgggcccag gcgtcgatct cggcgagcag ggcgatcttg tcggaaaagg cgggcgaggc
     2161 gaggggatcg acctcgtcat agagtttctg gttggtggcg cggggacctt cggcggtcac
     2221 gcccgcgtgg ccgcgcttgg cctgcgctgc gctgtcggcg gcgcggcgca gcgaggcggt
     2281 cgagatctcg tttgcgtggg cgtatccggc ggtctcaccg gcgacgacgc gcaggccgaa
     2341 gccctcggtg gcgtcatagg cggcggactt gagccgaccg tcgtcgaaca ccagcgactc
     2401 tgactccgag cgttcgagga aaagctcgcc atcgtcggcg ccgatcagcg cgttctgcag
     2461 gatcgagagg gcctcggacg ggtcgacccc ggcggtttcg aggatcgggg gcaggggcgt
     2521 gtggacggtc atgtccgata gatagggcgc gaaggcgcat tcgtcacggg tcgtgtcgcg
     2581 catctcctcc ccatcgcgca agcaatgggg aggaaagcgc taaccggatc agtcctgacg
     2641 gacgtccccg gagccggaga tcgactgacg cagcgtcgat ggccgggtgg cgaggtcaag
     2701 atcgccggag cccgacaggt tgatcaccgc agccccggtc ggggcgatcg tggcgtcgcc
     2761 gctgccggac gtatcgacct cggcgtcctc cgtcagcagg tccgaaaggt cagcgtcgcc
     2821 cgaaccggtc atcgtcaggg ccaacgcctt ggtcgcgccc gaagcggaga cgtcgccgct
     2881 gccggtagag gcgatggtca ggctcggctg atcgtatccc ttgatcgcca cgtcggccga
     2941 tccgttgatc tcgaaggtct tcacggccgg tgaggtgacc acgacgtgga tgtcgccgct
     3001 gtcgacccag aagccggccc ggttggcgtg gcggccgaag accagacgct cgtccttgtt
     3061 ggattcgtct ccagcccagc tcagacggtc gccgtcgatg cggacattat cgatccggtc
     3121 cgcacgaccc cggaccgtga tcccgggggt atccccctgg acgtattcga cgtcggcggc
     3181 atccagatcg acgaccagct tgtcgccgcc ggcccatgcc agggtgcggg tgacttcggc
     3241 aggctcggtc ccgtcctcac ggacaatatg caggtcgccg ccctcgccgt gactggggaa
     3301 ggtccactcc cagccgtgcc gggccatgtc ccgcccgccg acggcgacgc cgccggcgag
     3361 ggaggccaga gccagcacga aggctgcgcc ggcgatgatg agaagggtgc ggatcatacc
     3421 ggatctcccg aggtcgaggt cgccgcagtc ggagcggcgt ggggttcaag ggccggcttg
     3481 agcagccggt aatggaggcg ggcgaaccag atcaggccgt tgaccagcag gatggtgagg
     3541 atcgccagca aggcgcccgc agcgacggct cccgccatca ggccgaggcc gacgaagaag
     3601 gccgcgagac ctccgccggg gaagcctgcg aacgggccag cgaccatcac ggcgccgccg
     3661 gacacgaaca tggcgatgac cgcaaccagg atgccgaaca gcgtcccgat gacgcccatc
     3721 aggatcggca gcaggatgat gatgtcgagc gcgccgaggc ccagcaccgc gaacacggcg
     3781 ccggctgccg ccgacggcgt ctgttcctgg gtccagcgct ggacgccggc ctcggctttc
     3841 agttcacgcg ccagacggtc gggattcccc aaggcatccg cgacttcggc ctcggatcgc
     3901 ccggcagcct ttccgtcgtc gaaatgagcc tcgtagtcgt tgaggatctc ggtcgcggtc
     3961 gaggtcggca gtccgaccag gccccgcttg agccgggcga gaaagtcggc gcgcgtcatt
     4021 tcacatctcc agtggcgacg gtttcgaggg tcggcgcggg ggcggtctcg ttgagaaccg
     4081 cgtcgaccgc gtcggtgaag gtcttccagt cggcggtctg cacggcgagg gccgaccgac
     4141 cggcttcgga aagacgatag tatttgcgcg aggggccggc gctggactcg accagatagg
     4201 tctcgaccag tccgtctgac tgcatccgcc gcatcagcgg gtagatagtc ccttcgccca
     4261 tatcgatcgc gtccgacaat cggctggcga tctcgtaggc gtagctgtcg cctcgcttga
     4321 ggagggccag aacgcacagc gccagcactc ccttcttcaa ctgtatctct atggtttcag
     4381 gcaccggttg gtctcctctg atatccggag actacgacaa ggtatcatgc gtcacaaggt
     4441 agcttgcgaa acatgactgc gatttaatca tcgacccttt cggccgtgtg gcttcttcgc
     4501 ggtgctgatc gcttggcaag cggcaatcga gccgatatgg accggccgga gcgccgtgtt
     4561 caaagagcgc ggctggcgtt gcgactggct cgcaaacgcc tgttgcagat aggtttaagg
     4621 gatttttccg catgggactg gggcaggggc aaaacacagg gcgcgccctg aaagctgctg
     4681 gaggcctgat ggcctcggcc gccttcgccg tcctgaccgc atcgccggtt tgggctcagg
     4741 atcgtctggg gcagccgacg ccgggcggta tcgacctgca gccggccgct tcggtgctga
     4801 agcacgacgc gatctggttc cacaacgcga tcctgttgcc gatgtgtatc ggcatcagcc
     4861 tgctggtgct gggcctgatc gtctggatcg cgatccgcta caacaagaag tccaacccgg
     4921 tgcctgcgcg ctggagccac aacaccacga tcgagatcat ctggacgatc ctgccagtta
     4981 tgattctggt cggcatctcc ctgttctcat tccgcctgct gttcgcctac catgacatgc
     5041 cgacgccgga cctgaccatc aaggcgaccg gaaaccagtg gaactgggct tacgagtacc
     5101 ctgaccaggg catcgcggaa tacgtctcca acatcctgcc cgaagaagag ctggccaagc
     5161 gcggcatgcc gcacgacctg tatcgcctgg ccgccgacga gccgctggtg gttccagtcg
     5221 gcaaggtcgt tcgcgtgctg gtgaccgggg ccgacgtgat ccacaatttc gctctgccgg
     5281 cgttcggcct caagactgac gccgtccctg gccgggtcaa cgagacctgg ttccgcgccg
     5341 agcgtgaagg cgtcttctac ggccagtgct cggaactgtg cggcgacgac cacgccttca
     5401 tgccgatcga gatccacgtg gtctcgcaag ccgccttcgc ccagtgggtt gtctccaaag
     5461 gtggctcgat gacgccgaag acggcagccg aagctccggg cggcaccacc gctcagattg
     5521 cggccggcac gacgggcgct ccagtcgctc ccggagtgaa catggctgct ccggcgacga
     5581 ccgacggtcc tgctgctccc gtcggcgcca cgaccggcgc acccggtaac ggcccgtcaa
     5641 ccgcaatcgc accggcgacc acgtccgccg ccgctcccgc cgccccttcg gccgcccagt
     5701 aaccgcgagc ctacgaaaag atcatggcca ccgacatcac tgccgacgat cacgccgctc
     5761 cggggcacga cgcccatgcc gagcacgacc acaagcccgg gttctttacc cgctggtttc
     5821 tgtcgaccaa ccacaaggac atcggcaccc tgtacctgct gttcgccatc atggcgggca
     5881 tcgtgggcgg ggccctgtcc ggtctgatcc gctgggaact ggccgagccc ggcatccagc
     5941 tgttcaagga gggctcctgg gtccagtatc tcggcatcgt cgatgcttcg aagcacggct
     6001 ataacgctac ggtcaccgcc cacgccctga tcatgatctt cttcatggtc atgcccgcca
     6061 tgatcggcgg cttcggcaac tggttcgttc cgatcatgat cggtgcgccg gatatggcgt
     6121 tcccgcgcat gaacaacgtg tcgttctggc tgctggtcgc cgcctggtgc ctgttgatca
     6181 tgtcgatgtt cgcagacggc ggctcaggcc acgggattgg cgggggctgg accatctatc
     6241 ccccgctctc cacgaagggt tctccgggac ctgcgggcga cctcgcgatc ttcgccctgc
     6301 acgtcgcggg cgcatcctcg atcctcggcg ccatcaactt catcaccacc atcttcaaca
     6361 tgcgcgcgcc gggcatgacg ctgcacagga tgccgctgtt cgcctggtcg gtgctgatca
     6421 cggccttcct gctgctgctt tcgctgccgg tgcttgccgg cgccatcacg atgctgctgg
     6481 ccgaccgcaa cttcggcacc gctttcttcg atccggcagg cggcggcgat ccggtgatgt
     6541 tccagcacct gttctggttc ttcggtcacc ccgaggtgta catcctgatc ctgcctggct
     6601 tcggcattat ctcgcacatc gtgtcgacct tctcgcgcaa gccggtcttc ggctatctcg
     6661 caatggccta tgccatggtc gccatcggct tcatcggctt catcgtgtgg gcccaccaca
     6721 tgtacacggt cggcatgacg gtaaacctgc gggcctattt cgtcgccgcc accatgatca
     6781 tcgccgtgcc cacgggcgtg aagatcttca gctggatcgc gacgatgtgg ggcggttcga
     6841 tcgacttcaa gacgcccatg ctctgggcca tcggcttcat cttcctgttc accgtcggcg
     6901 gcgtgactgg cgtggtcctg tccaacgcgg gcatcgatta cagcctgcac gacacctatt
     6961 acgtcgtggc gcacttccac tacgtcctgt cgctgggcgc cgtgttcgcg atcttcgccg
     7021 gcttctacta ctggttcgag aagatgttcg gcgtgaagta caacgagttc ctcggcgcga
     7081 cccatttctg gatcatgttc gtcggcgtca acgtcgtgtt cttcccgcag cacttcctcg
     7141 gtctgcaggg catgccgcgt cgttatgtcg actatcctga cgccttcgcg ttctggaacc
     7201 ttgtgtcctc gatcggctat gtgatcacca tcgtcggcgt cggcgtgttc ctggtcatgc
     7261 tggccgaagc ggcgatccgt cgtcgtcccg gcgtcgccaa cccgtggggc gaaggcgcca
     7321 cgacgctgga atggaccctg tcgtcgcctc ctccgcatca ccagttcaac gaactaccgg
     7381 tggtcaagga agacgcacac tgactgaact cggggccggc gacagactgt cgtcggcccg
     7441 tttctcttct ggatccgggg cgtgacagag tgcgagccac ctcccgtttt cgaccgctac
     7501 gagcagcggg tacgcgaacg ccttctgaac ctgcgtcgcc tgatacttga tgtcgcagaa
     7561 acggagcggg tggggcctct tgaagaagtc ctcaaatggg gcgagcccag cttcgtgacg
     7621 catcagccca ggacgggcag taccatccgg atcaatgctg tcggacccag ccactacggc
     7681 ctgttcgtga actgcaagac gtcgctggcc gacaccttcc gcagtctcta tccggacgac
     7741 ctgaccgtta ttggcgatcg cgagttgcgc ttcggcatcg acgatgagct tccggagggg
     7801 ccggttcgcc gctgcatcgc ccttgcgctg acctatcacc gatggaaacc gcgctgacga
     7861 cgacttcgtt tcaccgcaac catcctgtag aagccccgtg atgaccgtcc ctaccgtctc
     7921 cgctctctcc accgcccagc cggaagactt cttccagctg ctgaagccgc gcgtgatgtc
     7981 gctggtggtg
//
LOCUS       DASVTM010000525         9696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_368160,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000525 DASVTM010000000
VERSION     DASVTM010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9696
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368160"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..365)
                     /locus_tag="VF633_14390"
     CDS             complement(27..365)
                     /locus_tag="VF633_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX8472297.1"
                     /translation="MKKIEAVIKPFKLDDVKEALQDIGVQGMTVLEAKGYGRQKGHSE
                     LYRGAEYVIDFLPKIKIEVVVADDLYPAVVEAIQTAARTGKIGDGKIFVTEVIEVIRI
                     RTGETGAQAV"
     gene            480..1298
                     /locus_tag="VF633_14395"
     CDS             480..1298
                     /locus_tag="VF633_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX8472298.1"
                     /translation="MTESNEPVLWRNNLPWPGLDVHKHQRGRLGVVSGAAYNTGAARL
                     AARAGLRIGAGLVRILCPLGAAAVIAPTVTAIMLDEFLSPEALGRLADSLDAVVIGPA
                     AGVNEETGYNLAALGRTGAAMVVDADALTVFRDRPKALFACLDRDDVLTPHEGEFERV
                     FPNLLKHGRENAAWEAARVAGAIIVLKGRETIIASPDGRLRVNRNGAAWLATAGSGDV
                     LAGMIAGLIAQRMDSFEAACAAVWMHADAANRFGAGLIAEDLPELLPAVLRDLR"
     gene            1390..1474
                     /locus_tag="VF633_14400"
     tRNA            1390..1474
                     /locus_tag="VF633_14400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1424..1426,aa:Leu,seq:tag)
     gene            complement(1530..3104)
                     /locus_tag="VF633_14405"
     CDS             complement(1530..3104)
                     /locus_tag="VF633_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8472299.1"
                     /translation="MWRVKSLDAILATAEKKSLHRSLGPIQLTLLGIGAIIGTGIFVL
                     TAAASQKAGPGMMLSFVIAGAVCAVAALCYSELAAMAPVSGSAYTYTYAVMGELLAWT
                     VGWALILEYAVAAAAVSVGWSGYVLGLIENGLGFDFPDLLSAGPVWAMQGFIPVPDFS
                     AGIINIPAIVVALLVTCLLMIGTTESARVNAVLVLIKIVALAVFIVITLPVVKAANLS
                     PFAPNGMFGAYSGMGVVGAAASIFFAYVGFDAVSTAAEETKNPQRNVPIGLIGSLAIC
                     TVFYLLVALGAAGAIGAQPVVGAAGEAVQPGSPAFVAACALQANAEALVCSNEALAHV
                     LRVIGHPQIGNALGTAALLALPSVILMMIYGQTRIFFVMARDGLLPEGLTKIHPKWKT
                     PYIVTAFTGIAVAIAAALFPVGQLADISNSGTLFAFFMVSIAVLVLRVKDPHRKRPFR
                     TPLLWVFAPLSAFGCAFLFWNLPHDAKMVLPIWGGIGLVIYFLYGYRKSHLGRGHMEV
                     HENDPDIPPPPATPMT"
     gene            3317..5749
                     /gene="hrpB"
                     /locus_tag="VF633_14410"
     CDS             3317..5749
                     /gene="hrpB"
                     /locus_tag="VF633_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEX8472300.1"
                     /translation="MLPIHAVLDDLKAALAGSDAVVLAAPPGAGKTTVVPLALLDQPW
                     LAGMKVLVLEPRRLAARAAAERMAKTLGEAAGDTVGYRTRLQSRIGPKTRIEVITEGV
                     FTRMILDDPGLEGVGAVLFDEFHERSLDADLGLALARESQSLLRPDLKLLVMSATLDI
                     AGVSGLLRGAPVIEAQGRMFPVETLYLGRNAAERFEDAMARACRLALTDQTGSILAFL
                     PGQGEIHRTLQRLNEQVRDPAVDIVALYGALDKAEQDRALDPSPPGRRKIVLATSVAE
                     TSLTIEGVRVVIDGGLSRVPRFEASSGLTRLVTVRVSKSSAEQRRGRAGRIESGVCYR
                     LWDEEQTRGLVPHQRPEILEADLTAFALDLARWGARSAEGLALLDPPPAGAFAEARNV
                     LIRLGALDEAGALTAHGRRLIGIPLTPRLAHLVAVAADAGDALLGARLAAVLSEPGLG
                     GNDVDIRERLRGLDRDRSPRARDAMKLADRWSRAAMKRAGRGGEADVGALLAAAFPER
                     IAKARGKPGEVLLASGRGAFLDPTDPLAREPWLAVAEVGGGDARDRIRLAAPVDPAML
                     EAQITTEDRLTREPSGRMVMRRIRRLGAIVVDEKITGAPDRAAITAALKAEVERGGLS
                     GLKWGERASGLRARLRWLSALEAGWPDVTDAGLLAEREIWLWPLLETVQSLENIPDGA
                     LEQGLRTLIPWDRQRAFDELAPARLTTPLGSAAIDYAAEGGPRVDIRVQELFGLKTHP
                     TVGGNARVPLTLALLSPARRPVQMTKDLPGFWSGSWSAVRADMRGRYPRHPWPEDPAS
                     ADPTNKVKPRGL"
     gene            5879..6865
                     /locus_tag="VF633_14415"
     CDS             5879..6865
                     /locus_tag="VF633_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX8472301.1"
                     /translation="MTTLRRLIDPYLLMLIATVAIAAVLPARGQGAVVAEWATTAAVA
                     LLFFLYGARLSPAAIWAGLSNWRLQSLVLAITFVLFPIVGLGVHALTGRWLTPEIAIG
                     LLYVCLLPSTVQSSIAFTSIARGNVPAALCSASVSNLLGVLITPILVSMLLPGSGGGV
                     SLKAIEDIALQILLPFALGQLARPVIGGLLIRHAKLAGYVDRGSILLVVYGAFSAGMV
                     AGVWAKLSPVDLGVVIVLNLVILAVIMVAATIASRRLGFSKEDEIAIVFCGSKKSMAS
                     GIPMAAILFAGQAVSLIVLPLMLFHQIQLFVCAVLARRYAERPMVASVQPAE"
     gene            6951..8237
                     /locus_tag="VF633_14420"
     CDS             6951..8237
                     /locus_tag="VF633_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8472302.1"
                     /translation="MNDADLLIPPSPASKRRVLFASLIGTTIEFFDFYIYAAAAVLVF
                     PKLFFPGGDPTTAMLQSFATFALAFFARPIGAVVFGHFGDRVGRKATLVAALMTMGLS
                     TICIGLLPTYADVGLLAPLLLALCRFGQGLGLGGEWGGAVLLATENAPPGRKAWFGMF
                     PQLGAPIGFILSTGAFIILTSTMSDETFLSWGWRTPFLASSILVLVGLWVRLRITETP
                     EFSRVIERAERVKAPILTLLGRYKMALLLGTLGATTTFLLFYLMTVFALGWATTAMGF
                     TRADILPIQLIGVIFFGALIPISALAADKFGQLTVLIAASIGIAIFGLLIAPLFSAGL
                     TGLLCFFSLGFALMGATYGPLSAAMARPFRAAVRYTGASVAFNLAGIVGASVAPFAAT
                     WLARNVGLPAVGLYLSATAIVTILALVGMTRLKVED"
     gene            complement(8255..9052)
                     /locus_tag="VF633_14425"
     CDS             complement(8255..9052)
                     /locus_tag="VF633_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8472303.1"
                     /translation="MTDERLITSLTNDTVKAVRALSMRKEREATGLFLAEGLKFIGEA
                     LDQGRTPKTLLVGMEARPHPLLDRAKAETIRTGGEVVIVTHPILEKISRRENPQTVLA
                     VFEQAFTPLSALKPETAPCWVALEQVRDPGNLGTIIRTADAAGCGGVILIGDCVDPFS
                     VEAVRATMGSVFAVTISRTTAAAFLAWRESWPGSVIGTRLDATHGYRDAAYAKPALIL
                     MGNEQAGLTDELAAACDVNVKIPMRGRADSLNLAIATGVMVYAVTDT"
     gene            complement(9049..>9696)
                     /locus_tag="VF633_14430"
     CDS             complement(9049..>9696)
                     /locus_tag="VF633_14430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8472304.1"
                     /translation="LKWRDVKFTGRFTPFRHLAFFPEQAANWEWLDARVRSLDKPRIL
                     NLFGYTGVASLAAAAAGAEVTHVDASKKSVAYARENAEASGLSDKPIRWIVEDARKYV
                     AREVRRGAKYQGIILDPPKYGRGPTGEVWRLFEDMPTLLKDCAALLANDASFLLLNAY
                     AARVSGLSLAHLMAEATHDRGGRIDWGELALSEDAQKDGSDARAIGLSFFARWSA"
BASE COUNT         1568 a         3043 c         3347 g         1738 t
ORIGIN      
        1 gatagacgtc cgccgggtgg ttgaactcaa acggcctggg cacccgtctc tccggtgcgg
       61 atacggatga cctcgatcac ctcggtgacg aaaatcttgc cgtcgccgat cttgcccgtg
      121 cgagcggcgg tctggatggc ctcgacgact gcagggtaga ggtcgtctgc gaccaccacc
      181 tcgatcttga tcttgggcag gaagtcgatg acgtattcgg ctccccggta gagttcggaa
      241 tgtcccttct gacgaccata tcccttggcc tccagcacag tcatgccttg cactccgatg
      301 tcctggagcg cctccttcac atcgtccagc ttgaaaggct tgatgacggc ttcaatcttt
      361 ttcatcgcaa tcccgaggcg gctcttgatt ggccagacgc tcaggtccga aagggacatc
      421 gcattgcacc ataaggcaag ccccatttgt attttccgtg tccgcggacg gagtgattga
      481 tgaccgaatc caatgagccg gtgctttggc gcaataatct gccgtggcca ggcctggatg
      541 tgcacaaaca tcagaggggg cgactcggcg tcgtctccgg cgcggcctac aatacggggg
      601 cggcgaggct ggcggccaga gcgggtctca ggatcggagc tgggctcgtg cgcatcctgt
      661 gtccgctcgg cgcggccgca gtcatcgcac cgacggtcac ggcgatcatg ctggacgagt
      721 tcctgtcgcc tgaagcgctg ggacggctcg cggattccct ggacgccgtc gtcatcggcc
      781 cggcggcggg cgtgaacgag gagaccggct acaacctggc ggcgctcggt agaacgggcg
      841 cggccatggt cgtggacgcc gacgccctga cggtgttcag ggatcggcca aaggccctgt
      901 tcgcctgcct cgaccgcgac gacgtcctga ccccgcacga aggcgagttc gagcgggtgt
      961 ttcccaacct gctcaagcat ggacgtgaga acgccgcgtg ggaggcggca cgggtcgccg
     1021 gggccatcat tgtgctaaag ggccgcgaga ccatcatcgc ttcgcctgac gggcggctga
     1081 gggtcaatcg gaacggcgcg gcgtggctgg cgacagccgg cagcggagac gtgctggcgg
     1141 gtatgatcgc cggcttgatc gctcagcgga tggatagctt cgaagcggcg tgtgccgccg
     1201 tctggatgca cgccgacgct gccaaccgct ttggcgccgg actcattgcg gaggacttgc
     1261 ctgagttgct tccggcggtt ctgcgcgatc ttcggtgaac tcgggcgagg cggctatccg
     1321 atccgtcttt gcaccggtgg ggagtggacg cagcgcattc ggtcgttgta gaggctcacg
     1381 cctaccgatg cggatgtggc ggaactggta gacgcaccag atttaggttc tggcatcgca
     1441 aggtgtggag gttcgagtcc tctcatccgc accaacacaa agaaaaaggc cccggattgc
     1501 tccggggcct tttcctcatc tgcaaccgat caggtcatcg gcgtcgctgg cggcggcggg
     1561 atgtccgggt cgttctcatg gacctccatg tggccacggc ccaggtggct cttgcgatat
     1621 ccatacagga agtagatcac caggccgatg ccgccccaga tcggcagcac catcttggcg
     1681 tcgtgcggca ggttccagaa caggaaggcg cagccgaacg cggacagagg tgcgaacacc
     1741 cagagcagag gcgtgcggaa cggccgcttg cggtgtggat ccttgacccg caacaccagc
     1801 accgcgatcg acaccatgaa gaaggcgaac agggtgccgg agttcgagat atcggccagt
     1861 tgtccgacag ggaacagcgc agcggcgatg gccaccgcga tgccggtgaa ggcggtcacg
     1921 atataaggcg tcttccactt ggggtggatc ttggtcaggc cttcgggcag caagccgtcg
     1981 cgcgccatga cgaagaagat gcgggtctgg ccgtagatca tcatcaggat gaccgaaggg
     2041 agggccagca gggcggcggt gccgagggcg ttgccgatct gtgggtggcc gatgacccgc
     2101 aggacgtgag ccagggcttc gttcgaacag acgagagcct cggcattagc ctgcagggcg
     2161 caggcggcga cgaatgcagg cgaaccgggt tgaacggctt caccagcagc gccgacgacg
     2221 ggttgagcgc cgatggcgcc agccgcgccg agggcgacca acagatagaa gacggtgcag
     2281 atggccagcg agccgatcag gccgatgggc acgttgcgtt gggggttctt ggtctcttca
     2341 gccgctgtcg aaacagcgtc gaaacccaca taggcgaaga agatcgaggc ggctgcgccg
     2401 acgaccccca tgcccgaata ggcgccgaac atgccgttcg gcgcgaacgg cgagaggttc
     2461 gcggccttca ctaccggcaa ggtgatgacg atgaagacgg ccagtgcgac gatcttgatg
     2521 agcacgagaa ccgcgttgac ccgtgccgac tccgtggtgc cgatcatcag caagcacgtc
     2581 acgagcagcg cgacgacaat ggccgggatg ttgatgatgc ctgccgagaa gtcgggaaca
     2641 ggaataaagc cctgcatggc ccagaccggg ccggcggata agaggtcggg gaagtcgaac
     2701 ccgagcccgt tttctatcaa ccccagcacg tagcccgacc agccgacgga aaccgcggcg
     2761 gcggccacgg cgtattcgag gatcagggcc caaccgacgg tccaggcgag aagttcgccc
     2821 ataacggcat aggtgtaggt gtaggcggaa cccgagaccg gggccatggc cgccagttcg
     2881 gaataacaga gcgccgcgac ggcgcagacg gcgccggcga tgacgaaact gagcatcatg
     2941 cccgggccgg ccttttgcga agcggctgcg gtcagaacga aaatgccggt gccgatgatg
     3001 gcgccgatgc ccaaaagggt gagctgaatt ggacctagcg accgatgaag agacttcttc
     3061 tcggccgtgg ctagaatcgc gtctagcgat ttaacgcgcc acatggatta cccctcgact
     3121 tttttgcccc ggcccctgcc ggtttgggcg gacgctaacc atggaaaacg gcttgcgcaa
     3181 cgcggtatca gcgcgatgac cgtggcacga cgctttagtg accccatgcg tggccgacaa
     3241 agcgcggcat tcccctctgg cgctaccggc attcgcgcta cttgagcgcg gctctttgcg
     3301 ctagttgagc cccgtaatgc tccccatcca cgccgttctc gacgatctga aagccgcgct
     3361 cgccgggtcg gatgcggtcg tgctcgcagc gcctccgggg gcgggcaaga cgacggtcgt
     3421 gccgttggcg ctgctggatc agccttggct ggcaggaatg aaggtgctcg tgctcgagcc
     3481 ccgacggctg gccgcacgcg ccgccgcgga acgaatggcc aagacattgg gtgaggctgc
     3541 gggcgacacg gtcgggtatc ggacgcggct gcagagtcgg atcgggccga agacgcggat
     3601 cgaggtcatc accgagggcg tcttcacccg catgatcctg gacgacccgg gtctggaggg
     3661 tgtcggcgcg gtcctgttcg atgagttcca cgagcgcagc cttgacgccg acctgggtct
     3721 ggcgctggcg cgtgagagcc agtcgctgct gcgccccgac ctgaagctgc tggtgatgtc
     3781 ggcgacgctg gatatcgcgg gtgtgtccgg gctcctcagg ggcgcgccgg tgatcgaggc
     3841 gcaggggcgg atgttcccgg tcgagaccct ctaccttggt cgcaacgccg ccgaacggtt
     3901 cgaggacgcc atggcgcgcg cctgccgtct ggccctgact gatcagaccg gttcgattct
     3961 ggcgttcttg ccgggccaag gcgagatcca tcgcacgctg cagaggctca acgagcaggt
     4021 gcgggatccg gcggtggaca tcgtcgccct gtacggtgcg ctcgataagg ccgagcagga
     4081 ccgggcgctc gatccgtcac cgcccgggcg tcgcaagatc gttctcgcca cctccgtcgc
     4141 ggagaccagc ctgaccatcg aaggcgtgcg ggtcgtcatc gatggcggct tgtcgcgcgt
     4201 gccccggttc gaggcgtcca gcggcctgac gcgactggtc acggttaggg tcagcaagtc
     4261 ctcggcggaa caacgccggg ggcgggcagg gcggatcgaa tccggcgtct gctaccggct
     4321 gtgggacgag gaacagacgc gcggtctggt cccgcaccag agaccggaaa ttctcgaggc
     4381 cgacctgacg gcgttcgcgc tcgatctcgc gcgctggggc gcgcggtcgg cagaggggct
     4441 ggcgttgctg gatccgccgc ccgctggagc cttcgcggag gcgcggaatg tcctgatccg
     4501 gctcggtgcg ctggatgagg cgggtgcgtt gaccgcgcac ggacggcggc tgatcggcat
     4561 cccgctgacc ccccgtctgg cgcatctggt cgccgtggcg gctgacgccg gggatgcgct
     4621 gttaggtgct cggctggcgg cagtgctgtc ggagccgggc ctcggcggca acgatgtcga
     4681 tatacgtgag cggctgcggg ggctggatag ggaccgctcg cctcgcgctc gcgacgccat
     4741 gaaactggca gatcgctgga gccgtgcggc gatgaaacgg gcagggcggg gtggcgaggc
     4801 cgatgtcggc gccctgttgg ccgcggcctt cccggagcgg atcgccaagg cgcgcggcaa
     4861 gccgggcgag gtgctgctgg cctcgggacg tggagccttt ctcgacccga ccgatccgct
     4921 ggcgcgtgag ccttggctgg ccgttgccga ggtcggcggt ggagacgcac gcgaccggat
     4981 tcgtctggcc gcgccggtcg atccggcgat gctggaagct cagatcacga ccgaggaccg
     5041 gctgacccgc gagccttcag gccggatggt catgcgccgg atccgcaggc taggcgccat
     5101 cgtcgtcgat gagaagatca caggcgctcc cgaccgcgcc gccatcaccg ctgcgctcaa
     5161 ggccgaggtt gaacgtggcg gcctgtccgg tctgaagtgg ggcgagcgag cttccggcct
     5221 gcgggctcga ctgcgctggc tgtccgcgct ggaggccggc tggccggatg tgaccgacgc
     5281 cgggcttctg gccgagcgcg agatatggct ttggccgttg ttggagacgg ttcagtcgct
     5341 ggagaacatc cccgacggcg cgctggagca agggttgcgg acgctgatcc cgtgggaccg
     5401 acagcgggcg ttcgacgaac tcgcgcccgc gcgcctgacc acgccgctgg gatccgccgc
     5461 catcgactat gcggctgaag gcggaccgcg cgtggatatc cgggtgcagg aactgttcgg
     5521 tctcaagacc cacccgactg tcggcgggaa cgctcgcgtg ccgttgacgc tcgccctgct
     5581 gtcgcccgcg cgccgtccgg tgcagatgac caaggacctg ccgggcttct ggtccggcag
     5641 ctggtccgcc gtccgcgccg atatgcgagg ccgctacccc cgccatccct ggcctgagga
     5701 cccggccagc gccgatccga ccaacaaggt aaagccaaga gggctgtaat gacgtctgct
     5761 ctactcagac gccgagcaag agaacgtcac ccttcgtgag gttgcacgct ttccgccaag
     5821 gttgagtctg ttcgctcgca ggcccaaacc gcccggacga catgacccgg aacgcctgat
     5881 gaccacgctg cgccgcctga tcgaccccta tctgctgatg ctgatcgcga cagtagccat
     5941 cgcagccgtc ctcccggcgc gggggcaggg ggcggtcgtg gcggagtggg cgacgacggc
     6001 agcggtggcg ctgctgttct tcctgtatgg cgcccggctt tcgccggctg cgatctgggc
     6061 ggggttgtcg aactggcggc tgcagtcgct ggttctggcc atcaccttcg tgctgtttcc
     6121 tatcgtgggg ctgggcgtgc atgcgctcac cgggcggtgg ctgacgccgg agatcgccat
     6181 cggcctgctt tatgtgtgcc tgctgccatc gacggtgcag tcgtcgatcg ccttcacttc
     6241 gattgcgcgc ggcaatgtcc cggcggccct gtgcagcgcc tcggtgtcca acctgctcgg
     6301 ggtgctgatc acgccgatcc tcgtgtcgat gctgctgccc ggttctggcg gcggagtctc
     6361 gttgaaggcc atcgaggaca tcgcgctgca gatactgctg ccgttcgcac tcggtcagtt
     6421 ggcccggccg gtgatcggag ggctgctgat ccggcacgcc aaactggcgg gctacgtcga
     6481 tcgcggctcg atcctgctgg tcgtctacgg agcgttcagc gcgggcatgg tcgcgggcgt
     6541 ctgggccaag ctcagccccg tcgatctggg cgtggtcatc gtcctgaacc tggtgattct
     6601 ggccgtcatc atggtcgcgg ccacgatcgc cagccgcagg ctggggttct cgaaagagga
     6661 cgagatcgcg atcgtcttct gcgggtccaa gaagagcatg gccagcggca tcccgatggc
     6721 cgccatcctg tttgcgggtc aagcggtcag cctgatcgtt ctgccgctga tgctgttcca
     6781 tcagatccag ctgttcgtct gcgccgtgct ggcgcggcgc tatgctgagc gaccaatggt
     6841 cgcttccgtt cagcccgccg aataaagcca cgcttcacag cttgacctga cgccaacctc
     6901 cgcgcatccg atgcatcaag gacgccccca atacgcccga ggatacgccc gtgaacgacg
     6961 ctgatctgct gatcccgccc agtccggcgt cgaagcggcg agtgctgttc gccagcctga
     7021 tcggcacgac gatcgagttc ttcgatttct acatctatgc agcggcggcg gtgctggtgt
     7081 ttccaaagct gttcttcccg ggcggcgacc cgacgacagc catgctgcag tcgttcgcga
     7141 ccttcgccct ggccttcttc gcccggccga tcggcgccgt ggtgttcgga catttcggcg
     7201 accgtgtggg acgcaaggcg acgcttgtgg cggccctgat gaccatgggg ctttcgacga
     7261 tctgtatcgg cctgctgccg acctacgccg acgtcggact gctggctccc ttgctgttgg
     7321 cactttgtcg gttcggccaa gggctggggc ttggcggcga gtggggcggg gcggtgctgc
     7381 tggcgaccga gaacgcccct ccgggacgca aggcgtggtt cggcatgttc ccgcaactgg
     7441 gcgcgccgat cggctttatc ctgtccaccg gcgccttcat catcctgacc tcgaccatga
     7501 gcgatgagac gttcctgtca tggggctgga ggacgccgtt cctggccagt tcgatcctcg
     7561 ttctggtggg tctctgggtg cgtttgcgga tcaccgaaac gcccgagttc agccgcgtca
     7621 tcgagcgcgc agaacgggtc aaggcgccca tcctgacgct tctgggccgc tacaagatgg
     7681 cgttgctgct ggggacgctg ggcgcgacga cgaccttcct gctgttctat ctgatgaccg
     7741 tgttcgcact gggctgggcg acgacggcga tgggcttcac gcgcgccgac atcttgccga
     7801 tccagctgat cggcgtgatc ttcttcgggg ccttgattcc gatctcggct ctggcggccg
     7861 acaagttcgg ccagttgacc gtgctgatcg ccgcctcgat cggcatcgcc attttcggcc
     7921 tgctgatcgc gccgctgttc agcgccggat tgacggggct gctgtgtttc ttctcgctcg
     7981 ggttcgccct gatgggagcg acgtatggtc cgctcagcgc cgccatggcc cggccgttcc
     8041 gcgcggcggt gcgttacacg ggcgcctcgg tggcgttcaa tctggcgggc atcgtcggag
     8101 cttcggtcgc gccgttcgca gcgacctggc tggcccggaa cgtcggccta ccagcggtcg
     8161 gcctgtatct gagcgccacg gcgatcgtga ccattctagc tctcgtcggc atgacccggt
     8221 tgaaggtcga ggactgagcc ccgaaagctg acagtcaggt atcggtgacc gcatagacca
     8281 tcaccccggt ggcgatggcg aggttcaggc tgtcggcccg gccgcgcatc gggatcttga
     8341 cgttgacgtc acaggcggcg gccagctcat cggtgaggcc ggcctgttcg ttgcccatga
     8401 ggatcagcgc aggcttggca tacgccgcgt cgcgatagcc gtgggtcgcg tccagacggg
     8461 tgccgatgac actgccgggc cagctttcac gccaggccag aaacgcagcg gccgtcgtcc
     8521 tcgatatggt cacggcgaag accgagccca tggtcgcgcg caccgcctca accgagaacg
     8581 gatcgacgca gtcgccgatc aggatgacgc cgccgcaacc cgccgcgtcc gccgtacgga
     8641 tgatggtgcc gagattgccc ggatcgcgta cctgttccag cgccacccag cagggcgcgg
     8701 tttcgggctt cagcgcggac agcggcgtga aggcctgctc gaatacggcc agcaccgtct
     8761 gcgggttctc gcggcggctg atcttctcga ggatcggatg ggtgacgatg acgacctcac
     8821 cgcctgtccg gatggtttcg gccttcgcgc ggtccagcag cggatgcggt cgcgcctcca
     8881 tcccgacaag cagggttttc ggcgtccgac cctgatcaag tgcttcgcct atgaacttca
     8941 gcccctcggc gagaaacagc cccgtcgcct cgcgctcctt gcgcatactc aacgcgcgaa
     9001 cggccttaac cgtgtcgttg gtcagggagg tgatcagacg ctcatcagtc atgccgacca
     9061 cctcgcgaag aagctcaggc cgatggcgcg ggcgtcgctg ccgtctttct gtgcgtcttc
     9121 ggacagggcc agttcgcccc agtcgatccg gccgccccgg tcatgggtcg cctcagccat
     9181 cagatgggcg agcgacaggc cggagacgcg ggcggcatag gcgttcagca gcagaaacga
     9241 cgcgtcgttg gccagcaggg cggcgcagtc cttgagcagg gtaggcatgt cctcgaacag
     9301 tcgccaaacc tcacccgtag gaccgcgtcc gtatttgggc gggtcgagaa tgatgccttg
     9361 atatttggcc ccgcgacgga cctcgcgcgc gacgtatttg cgggcgtcct cgacgatcca
     9421 gcggatcggc ttgtcggaca ggccggaggc ttcggcgttc tcgcgggcat aagcgaccga
     9481 cttcttggag gcgtcgacgt gggtgacttc ggctccggcg gcagcggcgg ccagcgaggc
     9541 gacgccggtg tagccgaaca ggttcaggat gcgcggctta tccaaagacc ggacccttgc
     9601 gtcgagccat tcccagttcg cggcctgttc gggaaagaag gcgaggtggc ggaagggcgt
     9661 gaagcgaccg gtgaacttta cgtctcgcca cttcag
//
LOCUS       DASVTM010000526        10090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_475199,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000526 DASVTM010000000
VERSION     DASVTM010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475199"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..957)
                     /locus_tag="VF633_14435"
     CDS             complement(127..957)
                     /locus_tag="VF633_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8472305.1"
                     /translation="MSYVTTNDGVEIFYKDWGPKDAQPIVFHHGWPLSSDDWDGQMMF
                     FLSKGFRVVAHDRRGHGRSSQVSDGHDMDHYAADAAAVYEHLGLTNAVHIGHSTGGGE
                     VVRYVVKHGAGKVAKAVLVSAVPPLMLKTETNPEGLPIEVFDGFRSSLAANRAEFYKA
                     VASGPFYGFNRSGVTTLQGVVDNWWRQGMIGGAKAHYDGIKAFSETDFTEELKVMNVP
                     TLVLQGDDDQIVPYKDASLKAAALLKNGTLKIYPGYPHGMLTTHGDVINPDLLAFIQA
                     "
     gene            1330..1863
                     /locus_tag="VF633_14440"
     CDS             1330..1863
                     /locus_tag="VF633_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX8472306.1"
                     /translation="MPDLKTADDVRQRPLRTPTDLKPEATRDISAGLSALLADVFAVY
                     LKTKNFHWHMSGPNFRDYHLLLDEQGDQLFAMTDPIAERARKIGGTTIRSIGQIARQS
                     RILDNDADYVDPKDMLSELREDNQQLVGAMRQVHDVCDEYGDVATASLLENWIDETER
                     RTWFLFEITRAAPGVNY"
     gene            complement(1950..2312)
                     /locus_tag="VF633_14445"
     CDS             complement(1950..2312)
                     /locus_tag="VF633_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472307.1"
                     /translation="MSRIFVSIVDDDAGSRGATASLVRSLGLAVDVFASAREFLASPS
                     WVTTACLITDIHMPEISGLELQTRLRALRPDCPVLVVTGFPTEAVRDMAIEGGAAGFF
                     AKPLGSDFLTCLEGQITT"
     gene            complement(2378..3043)
                     /locus_tag="VF633_14450"
     CDS             complement(2378..3043)
                     /locus_tag="VF633_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8472308.1"
                     /translation="MSDQTADTVGSGPVVYVLDDDAEVRGALSSLLRSAGHGVQTFES
                     TEAFLYGISPTRPSCLLLDIRLGDDDGLEFQNRLKDVDITIPVILMTGHGDIPMTVRG
                     MKAGAIDFLAKPTDDGALLAAVDTALAADAQRRLSRSQSDTLEQLYASLTNREREVMG
                     LVTAGLMNKQVAGKLGLSEITVKIHRGHVMRKMQADSLADLVRMAELLGGRATDASRY
                     TSV"
     gene            complement(3040..4935)
                     /locus_tag="VF633_14455"
     CDS             complement(3040..4935)
                     /locus_tag="VF633_14455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8472309.1"
                     /translation="MSSRPKTQPSPLLRLALPVLIAAAIFLVDSLTRFGGAVAVLYTV
                     VILLVANGSGRTRILWAGAGCVVLTLIAFLPDHFGEPWSSSHARLIVSLAAITIITFL
                     AVRTRSAEANFAAQARVLDLTHDTVIIHRADPGDEDIVCYWNDGAADLFGFSREEAVG
                     AACQDLLRTELPVPRAEIDAIIATAGAWSGELIRTRKDGARLILSSRWSARQGARGRE
                     IIETSADVTAQRLLDRRYQGIFNAAGFAIWEADWSLIRRAILDLKQQGIGDLEAYLLA
                     HPAFVRQMVQDVAILDINAVAVRLFEADSREELIGHSLARSFTATTETAFARALAKVD
                     AGAQQVELETLYQTLKGNPLEVLIRVTVPDSDGAWDRVLVTALDLTERNRMQTRLNET
                     LAELSHASRISMLGELAASIAHEVNQPLAAVVTYGEAGRRWLARDPLDRAELSDCIEH
                     IMANGRRATDVIGRVRSLAKQGQGQIARLSLAATATEAGALLERELRAHGVRYHEEIA
                     PDLPEIDGDRVQLQQVIINLLMNAVQAMQTTPEVERILILSISTPGEAHVRLEVRDHG
                     PGIAPEQAGRLFQSFATTKADGLGMGLSICRSIIEAHHGQISLTNHDDGGASAVILLP
                     VPAPEAT"
     gene            5174..6853
                     /locus_tag="VF633_14460"
     CDS             5174..6853
                     /locus_tag="VF633_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8472310.1"
                     /translation="MTETDSRHEQRFPVLEPAALEAAMRFTSGPVHVFAPGEVVFGIG
                     DLESPVWLIIEGSIIAVRRDGLGHEKMIVTQTAGQFTGEVSQLAGRASLAQGTAGPEG
                     CTAAPIDAAHLRALMIGSADIGEVVMRALILRRVGLIEGGSAGSVLVGRPGDANLLRL
                     QGFLTRNGYPNTLLDADSDAEGRAIVERLGVAAEDLPLMVCPNGTVLNRPSETEAAIC
                     LGMTPDLDPAKVYDVAIVGAGPAGLATAVYAASEGLSVLVLDQRAIGGQAGASARIEN
                     YLGFPTGISGQALAGRAYNQALKFGAELAIPLEVARMDCASDGLGHPHRLELTDGQIV
                     CARTVVVASGARYRRPDIENLAPFEGSGVSYWASPVEARLCAGEEVALVGGGNSAGQA
                     VIFLASKVKALNLVVRGAGLEASMSRYLIDRIAALPNVTIHTHTEVTALEGDLNSGLV
                     GAVFRDRRTGETHRRDLHHLFLFVGADPNAAWLQGCVTLDDKGFVNTGTSSLSLQTDR
                     AGVFAIGDVRAGSTKRVAAAVGEGAAVVSQIHQYLASLDDEARANREGTGS"
     gene            6850..7155
                     /locus_tag="VF633_14465"
     CDS             6850..7155
                     /locus_tag="VF633_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX8472311.1"
                     /translation="MTDGCRHMATVRTVTPSARGCEDCLKIGSAWLHLRLCRECGHVG
                     CCDQSPHRHATAHFHATRHPIIEGYDPPEGWGWCYVDEVFVDLPDQTPQLGPIPRYY"
     gene            7193..8380
                     /locus_tag="VF633_14470"
     CDS             7193..8380
                     /locus_tag="VF633_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8472312.1"
                     /translation="MAQAEPESLSAVAPLARFTLFAMAVAAGLAVANIYYNQPMLGLM
                     ERDLPGPLTRFTPTVTQFGYALGLFLLLPLGDIVERKRLIVIQFVVLALALAAVAVAP
                     GAALVVAASLVVGIAATVAQQIVPFAAHLSAPEKRGVTVGTVMAGVLCGILLSRTLAG
                     LVATHAGWREMFWLGAPLAVVAGGLMLVVLPTSRPDSRLSCPALMKTLIHLWREFPAL
                     RRAAFTQALLFGAFTAFWTILALRLQEPRFNLGADVAGLFGVVGAVGILAAPLAGRIA
                     DRSGPRPVIALGAVLALGAWILFGLWTSIAGLVVGVILLDFAVQSALVSNQHVVYALR
                     PAARARLNTLFMGAMFLGGSAGSALATLVWGAAGWTGVSLLGVAFTAVAAVIQIIDLR
                     RRV"
     gene            8416..9147
                     /locus_tag="VF633_14475"
     CDS             8416..9147
                     /locus_tag="VF633_14475"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8472313.1"
                     /translation="MTTVADLIAKTLASAGVRRVWGVTGDSLNGLTDSLRRHGDIDWM
                     HVRHEETAAFAAGADSAVTGALAVCAGSCGPGNLHLINGLFDCHRNRVPVLAIAAHIP
                     SSEIGLGYFQETHPQELFRECSDYVELVSNPAQIAGVLARALNTAVGKGGVAVIVLPG
                     DVALSETPVEALDWRPPARPRTVPATSDITRAADILNSVPIGLGLVIGVERLPLRQKL
                     GKLGLGEASQTASCKTAEFSFYQFQ"
     gene            9458..>10090
                     /locus_tag="VF633_14480"
     CDS             9458..>10090
                     /locus_tag="VF633_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8472314.1"
                     /translation="MPIITGTSGADTLTGTADEDTIDGLAGNDRISGGGGNDMLIGGD
                     GFDTLNGEAGNDSIQGDTGLDFIEGGLGDDVMDGGTGGERTPQLVGVGTVVNGGDVAS
                     YRTATAGVRVDLRLSGQQDTGGAGIDTLINFEDISASDFNDVLFGNDAANLISGAFGD
                     DQIFGLGGNDVLLSSLGNDLVDGGDGDDFIWAGQLGQQPYVNAGNDTLIGG"
BASE COUNT         1787 a         3139 c         3273 g         1891 t
ORIGIN      
        1 gctgtatcgg gcggctcgag catcgggcgg caaggtcgac cagctacatg cggcggagtc
       61 acattgaacg atggtgctga cgcccctcca gctgagccgc gccaaggtgg cgcggctcaa
      121 cttcagtcag gcttgaatga aggccagaag gtccgggttg atgacatcgc cgtgggtggt
      181 caacattccg tggggatagc ctgggtagat cttcagcgtg ccattcttca gcaaagcggc
      241 cgctttcaac gaagcgtcct tgtaggggac gatctgatcg tcatcaccct gcaacaccaa
      301 cgtcgggacg ttcatgacct tcagctcttc agtgaagtcg gtctcggaaa aggccttgat
      361 gccgtcgtag tgggccttgg cgccgccgat catgccctga cgccaccagt tgtcgacgac
      421 accctgcagg gtcgtcacgc ccgaccgatt gaagccatag aaggggcctg aggcgactgc
      481 cttgtagaac tcggcgcgat ttgcggcgag gcttgaccga aagccatcga acacttcgat
      541 cggcaggcct tcgggattgg tttcggtctt gagcatcagc ggcgggacgg cgctgacgag
      601 gaccgccttg gcgactttgc cggcgccgtg cttgaccaca taccggacga cttcgccgcc
      661 acccgtggag tggccgatat ggaccgcatt cgtcaggccg agatgctcgt aaacggcggc
      721 ggcgtctgcg gcgtaatggt ccatgtcgtg gccgtcggag acctggctgg accgaccatg
      781 accgcgacgg tcgtgagcga cgacgcggaa acccttggac aggaagaaca tcatctgacc
      841 gtcccagtcg tccgagctga gcggccagcc gtggtggaaa acgatcggtt gggcgtcctt
      901 gggaccccag tccttgtaga agatttcgac gccgtcgttg gtggtgacat agctcatggt
      961 cttgtcctcc tgatgtggcc cggcattggg ctgtggggtt ttggaagtcg cctcggcgtt
     1021 cgctgctgtg agtgacaggc cagcgatagc cgcaccgccg atcaagatcc gacggcggtc
     1081 ggtgatggcg atcggttgga agttctgaaa cgaggtcatg ggttggcgct cactgctgat
     1141 gtctttggaa aagagaccgg cggttcgctt ccgaagcccg gcgacattca gacgctaggc
     1201 gtcgttccaa agaggctcaa tcaaccgaaa gtatgtgaaa ccttcgtatg gctaggcctc
     1261 tgcatctgca ggtctaggaa ggggccatgg ccgttttccg gccaccagct atttgccccg
     1321 gagccgaaga tgcctgacct gaaaactgct gacgatgtcc gccaacgccc tctgcggacc
     1381 ccgaccgatc tcaagccgga agcgacccgc gacatctccg ccggcctgag cgccctgctt
     1441 gcggatgttt tcgccgtcta cctgaagacc aagaactttc actggcacat gagcggcccg
     1501 aacttccgcg actatcacct gctactggac gagcagggcg accagctgtt cgccatgacc
     1561 gatccgatcg ccgagcgggc gcgcaagatc ggcggaacga caatccgctc gatcggccag
     1621 atcgcgcgtc agagccgcat cctcgataat gacgcagact atgtcgatcc gaaggacatg
     1681 ctgtcggagc tgcgcgagga caatcagcag ctggtgggcg ccatgcgcca ggtgcacgac
     1741 gtttgcgatg aatacgggga cgtcgcgacg gccagcctgc tggagaactg gatcgacgag
     1801 acagaacggc ggacgtggtt cctgttcgag atcacccgcg ccgcgccggg cgtgaattac
     1861 tgatcttgaa gtccagaagc gggcgggcgt cggaaacggc gcccgctttt tgctgtgtgg
     1921 ggccgaacgc gccgccgcag gatcaacggt caggtagtga tctggccttc gaggcaggtc
     1981 aggaaatcgg agccgagcgg tttggcgaag aagccggcag cgcctccctc aatcgccatg
     2041 tcgcgcacgg cttcagtcgg gaatccggta acgacgagca cagggcagtc gggccgcagg
     2101 gcgcgcagtc gtgtctgaag ttccagcccg ctgatctcgg gcatgtgaat gtcggtgatc
     2161 aaacacgccg tcgtcaccca ggatggcgat gcgagaaact cacgagcgga agcgaagacg
     2221 tccacggcca gcccgagcga tcgcaccaga ctggctgtcg ctccgcgact accggcgtcg
     2281 tcatccacta tcgaaacgaa aatacgggac acccggactc ctcgcggtcg tcgccgcaat
     2341 tggggtccct tatcaatctt ttagcgccgg gaaacgatca tacggacgta tagcgcgagg
     2401 cgtcagtcgc ccgtccgccc agaagttcgg ccattcgcac cagatcggcc agtgaatccg
     2461 cctgcatctt acgcatgacg tgaccgcggt gaatcttgac ggttatctcg ctgaggccga
     2521 gcttgccggc gacctgcttg ttcatcaggc ctgcggtgac caagcccatc acctcgcgtt
     2581 cgcggttggt cagcgaggca taaagttgct caagcgtgtc ggactgcgac cggcttaagc
     2641 gtcgttgcgc gtcggcggcc agcgccgtgt ctacggccgc cagcagggcc ccgtcgtcgg
     2701 ttggcttggc caggaagtcg atcgcacccg ccttcatccc gcgcaccgtc atcggaatat
     2761 cgccgtggcc cgtcatcagg atcaccggga tcgtgatatc gacgtccttc agccggttct
     2821 ggaactccag cccgtcgtcg tcgcccagtc ggatgtccag gagaaggcat gagggtctcg
     2881 tcggtgatat cccgtacagg aaagcctcag tgctctcgaa ggtctggacc ccatgaccgg
     2941 cggatctcaa caagctggac agggcgccac gcacctccgc gtcatcgtcg agaacataga
     3001 cgaccggacc ggagccaacg gtgtctgcag tctggtcgct catgtggcct ccggtgcggg
     3061 aacagggaga aggatgacgg cggaggctcc gccatcatcg tggttggtca gactgatttg
     3121 accatggtgc gcctcgatga tggagcggca gatagacagg cccataccca ggccgtccgc
     3181 cttcgtggtg gcgaaggact ggaacaaccg cccagcctgt tccggcgcaa tgcccggccc
     3241 gtggtcgcgc acctcaaggc gaacgtgagc ctcgccaggc gtcgaaatcg aaaggatcag
     3301 gattcgttcg acctccggtg tcgtctgcat ggcttgaacc gcgttcatca gcagattgat
     3361 aattacctgc tgcaactgga cccgatctcc atcgatctcg ggcaggtcgg gagcgatctc
     3421 ctcgtgatag cgaacgccgt gcgcgcgtaa ctcgcgctcc agcaaggcgc cggcctcggt
     3481 tgcggtggca gcaagggaca agcgggcgat ctgaccctgt ccttgcttcg ccagactgcg
     3541 gacacgtccg atgacgtcgg tagctctgcg accgttggcc atgatgtgct cgatacagtc
     3601 ggagagctcc gcccggtcga gggggtcgcg cgccagccag cgccgacccg cctctccata
     3661 ggtgacgact gccgcgagcg gctggttcac ttcatgcgca atggacgccg ccaactcgcc
     3721 cagcatcgag atccgtgagg cgtgggacag ctcggccaga gtctcgttca gtctggtctg
     3781 catcctgttg cgttcggtca ggtccagcgc agtgaccagc actcgatccc aagccccgtc
     3841 gctgtctgga acggtgacgc ggatgagcac ctcgagcggg ttgcctttga gcgtctgata
     3901 gagggtttcc agttcgacct gttgggcgcc ggcgtccacc ttcgccagcg cccgggcgaa
     3961 ggccgtctcg gttgtagcgg tgaaactgcg ggcgaggctg tgaccgatca gttcctcgcg
     4021 actgtccgcc tcgaaaagcc ggacagcgac cgcgttgatg tcaagaatgg cgacgtcctg
     4081 gaccatctgt cggacaaacg cgggatgcgc gagcaggtag gcttccagat ccccaatgcc
     4141 ttgttgcttg aggtcgagga tcgcgcgacg gataagggac cagtcggctt cccatattgc
     4201 gaaacccgcg gcgttgaaga tgccctgata gcgacggtcc agcagcctct gtgcagtgac
     4261 gtcggcgctg gtttcgatga tttcccggcc tcgcgcgcct tgtcgggcag accagcggct
     4321 ggagagtatc agccgggcgc catccttgcg ggtcctgatg agctcgcccg accacgctcc
     4381 ggcggtagcg atgatcgcgt cgatctcggc acgcggcaca ggcagttcgg tgcgtagaag
     4441 gtcctgacag gccgcgccga cggcttcctc acgagagaag ccaaagagat ccgccgcacc
     4501 gtcgttccag tagcaaacga tgtcctcatc accggggtct gcccggtgaa tgatgaccgt
     4561 gtcgtgggtc agatcgagca cgcgagcctg agccgcgaaa ttagcctcgg ctgagcgcgt
     4621 gcgcaccgcg aggaaggtga tgatggtgat cgcagcgaga ctcacaatca gccgtgcgtg
     4681 actgctgctc cagggttctc cgaaatgatc aggcaaaaac gcgatcaggg tcaggacgac
     4741 gcaaccggcg ccggcccaga ggattcgcgt ccgtccgcta ccgttggcga ccagaaggat
     4801 caccacggtg tagagcaccg cgaccgcgcc cccgaagcgc gtgagggagt ctacgagaaa
     4861 gatcgccgcc gcgatcagaa ctggaagggc gaggcgaaga agcggggagg gctgcgtctt
     4921 tgggcggctg ctcaacaaat cccccttgtt cgcaggtcca gtcatccgtg aaaggcaggc
     4981 ttagccattc cgctcccggg cgtccatgcc tgcgtcgggt gcccatcata ccaacgtatt
     5041 cttggcggca agagccgaac ggctcatcca taatcggaag ccggtactat cggcgtctgt
     5101 accggcgatg tgatgagcac agtccgtaca gcgccgcgaa ccgctaccat tcgggacgac
     5161 acggggacgc acaatgaccg agaccgacag ccgccacgaa caaagatttc cggtccttga
     5221 acccgctgct ctggaagccg cgatgcggtt caccagcggt cctgtccatg tgttcgcgcc
     5281 gggggaggtt gtgtttggaa taggcgatct tgagagtccg gtctggctta tcatcgaggg
     5341 ttcgatcatc gccgtgcggc gcgatggtct cgggcacgaa aagatgatcg tcacgcagac
     5401 tgcaggccag ttcactggcg aagtcagtca gttggccggc cgcgcatccc tggctcaggg
     5461 aacggccgga ccagaaggat gcaccgccgc tccgatcgac gccgcccacc tccgcgccct
     5521 gatgatcggc tcggcggata ttggcgaggt ggtcatgcgc gcgctgatcc ttcgccgggt
     5581 cggcttgatc gaaggcggaa gcgccggttc ggtgctggtc ggccggccgg gcgacgcaaa
     5641 cctgttgcgc ctgcaaggct ttctgacccg gaatggctat cccaacaccc ttctggatgc
     5701 cgacagtgac gccgaggggc gtgccatcgt cgagcgtctg ggcgtagcgg cagaggacct
     5761 gccgctgatg gtctgtccca atggcacggt gctgaaccgc cccagcgaga cggaagccgc
     5821 catctgtctg ggcatgacac ccgatctcga tccggcgaag gtgtacgatg tcgccatcgt
     5881 cggcgcagga cccgcaggtc tcgcaaccgc cgtctacgct gcgtctgagg ggttgtcggt
     5941 gctggtgctg gaccagcgcg ccatcggcgg ccaggcaggc gcttctgccc ggatcgagaa
     6001 ctatctcggt ttcccgactg gcatctcggg tcaagccctt gcggggcgag cttacaatca
     6061 ggcgctgaag ttcggcgcgg agctggcgat cccgttggaa gtggcgcgta tggactgcgc
     6121 ctcagacggt ctcggacatc cgcatcgtct cgaattgacg gacgggcaga tcgtgtgcgc
     6181 acgcaccgtg gtggtggcgt caggcgctcg ttatcgccgt cccgacatcg agaaccttgc
     6241 cccgttcgag ggttcgggcg tgtcttactg ggcctcgcca gtcgaggcgc gcctgtgcgc
     6301 gggtgaggaa gtcgccctgg taggcggcgg caactccgct ggacaggcgg tgatcttcct
     6361 cgcgtcgaaa gtcaaagctt tgaacctcgt tgtgcgaggg gcaggacttg aagcgtcgat
     6421 gtcgcgttac ttgatcgacc ggatcgcggc cttgcccaat gtcacgatcc acacccatac
     6481 cgaggtcaca gccttggagg gcgatctgaa cagcggattg gtcggcgccg tcttcaggga
     6541 ccgccgcacc ggcgagaccc acagacgcga tttgcatcat ctcttcctgt tcgtcggggc
     6601 cgatcccaat gcggcttggc tgcagggttg cgtcacgctt gacgacaagg ggttcgtcaa
     6661 caccgggacg tcgtctctgt cgctgcagac ggaccgggcc ggggtctttg cgattggcga
     6721 cgtccgcgcg ggctcgacaa aacgggtcgc cgctgcggtc ggcgaaggcg cggctgttgt
     6781 cagccagatt caccaatatc tcgcctccct cgatgatgag gctcgggcaa accgcgaagg
     6841 aaccggatca tgaccgacgg atgccgccac atggcgacgg tgcgaaccgt gacgccgagc
     6901 gcgcgcggat gcgaggactg cctgaagatc gggtccgcgt ggctgcacct tcggctttgc
     6961 cgcgagtgcg ggcatgtcgg ctgctgcgat cagtctcccc accggcacgc gaccgcccat
     7021 ttccatgcga cccgccatcc gatcatcgag ggctacgatc cgcctgaggg atggggctgg
     7081 tgctatgtcg acgaggtctt cgtcgatctg cccgaccaga cgccgcagct cggcccgatc
     7141 ccccgatatt attgaacgtc cttccgagcc cctgaacgca gactgatact caatggcgca
     7201 ggccgaacca gaatccctat ccgccgtcgc cccgctcgcg aggttcaccc tgttcgccat
     7261 ggccgtcgcc gcagggctgg cggtggccaa catctattac aaccagccga tgctgggtct
     7321 gatggaacgg gacctgcccg gtccactcac ccgtttcacc cccacagtga cccagttcgg
     7381 ctacgcgctc ggcctgttcc tgctcttgcc cttgggcgac atcgttgaac gcaagcggct
     7441 gatcgtgatc cagttcgtcg tcctggcgct ggcgcttgcg gcggtcgccg tcgcgccggg
     7501 cgccgcattg gtggtggctg cgtcgctggt cgtcgggata gcggcgacgg tggcccagca
     7561 gatcgtgcct ttcgccgcac acctgtccgc ccccgaaaag cgcggggtaa cggtcggaac
     7621 cgtgatggcg ggggtcttgt gcggcatcct gctcagccgc acgctcgccg gccttgtcgc
     7681 gacccatgcg gggtggcgcg aaatgttctg gctcggcgcg ccgctggccg tcgtcgccgg
     7741 gggtctgatg ctcgtcgttc tgccgaccag ccgccccgac agccgcttgt cctgtccggc
     7801 cttgatgaag accctgatcc atctgtggcg ggagttcccg gccttgcggc gggccgcctt
     7861 cacccaggcc ctgctgttcg gcgccttcac cgccttctgg accattctgg cgctgcggct
     7921 gcaggagccg cggtttaacc tcggcgctga tgtagccggc ctcttcggtg tggtcggcgc
     7981 cgtcggcatt ctggccgcgc cgctcgctgg ccggatcgcg gatcgcagcg gcccgcgtcc
     8041 ggtcatcgcc ctgggcgccg tcctggcact gggggcctgg atactgttcg ggttgtggac
     8101 ctcgatcgcg ggtctcgtgg tcggcgtcat tcttctggac ttcgcggtgc agagcgccct
     8161 cgtgtccaac cagcacgtcg tctatgcgct gcggcccgcg gcgcgggcgc ggctcaacac
     8221 cctgttcatg ggggcgatgt tcctcggcgg ttccgcaggc tccgccctgg cgaccctggt
     8281 ctggggcgcc gccggatgga ccggcgtcag ccttctcggc gtggccttca ccgctgtcgc
     8341 cgccgtgatc cagatcatcg acctcagacg gcgcgtctga gcgatttcgc gcactcgctt
     8401 gcaaggacat gatccatgac cactgtcgct gatctgatcg caaagacctt ggcctcggcg
     8461 ggtgtgcggc gcgtctgggg tgtcaccggc gacagcctga acgggctgac cgacagcctg
     8521 aggcggcacg gcgatatcga ctggatgcac gtccgccacg aggagactgc cgccttcgcc
     8581 gccggggccg attcggcggt gaccggcgca cttgcggtct gcgcgggcag ctgcggtccg
     8641 ggcaatcttc acctgatcaa cggtttgttc gactgtcacc gtaaccgcgt gccggtcctg
     8701 gcgatcgccg cgcacatccc gtcgtcggag atcggtctcg ggtattttca ggagactcat
     8761 ccgcaggagc tgttccgcga gtgcagcgac tatgtcgaac tggtctccaa cccggcccag
     8821 atcgctggcg tcctggcccg cgccctcaac accgccgtcg gcaagggcgg ggtggccgtg
     8881 atcgtcctgc cgggcgatgt ggccctgtcc gagaccccgg tggaggccct cgactggcgt
     8941 ccacccgccc ggccccgcac ggtgccggcg acctcggaca tcacgcgagc ggcggatatc
     9001 ctcaacagcg tcccgatagg ccttggactc gtaataggtg tcgagcgtct tccactgcgc
     9061 cagaagcttg ggaagctagg tcttggcgag gcttcacaga cagctagctg caaaactgca
     9121 gaattttcat tctatcaatt tcaatgagtt attggggaaa aacaaatcgg aggagggtgt
     9181 ctccagacgc cctcgttaca gccggcctcg tgagcaccgt gagttccgac ggtcacagcc
     9241 cgggttgtcg ctggttcaag ggcatctcgg gttacgacga gccaaggacc gcgacctcaa
     9301 cgctggagag cgggtcagca tcagtccaag gagtcagacc ttgtcctcct actggttcga
     9361 ttatcaaaga cacatgacca ccctgttgca gcttgcgatg ccaacctgag gctgcaaaag
     9421 tccttcggta acgctttgtc gctcgtagga gttcgtcatg cccatcatca ccggaacctc
     9481 cggagccgac accctcacag ggacggcaga tgaggacacg atcgacggcc tcgccggtaa
     9541 cgaccggatc agtggcggcg gcggcaatga catgctgatt ggcggcgatg gatttgacac
     9601 cctaaacggt gaagccggca acgacagtat tcagggtgac acagggctag attttattga
     9661 aggcggcctt ggcgatgacg tcatggatgg cgggacgggc ggagaaagga ctcctcagtt
     9721 ggtcggagtc ggcaccgtcg taaatggcgg cgacgtggcc agctatcgca cagcgactgc
     9781 gggcgttcgg gtggatctgc gcctcagcgg tcagcaggac accggcggcg cgggcatcga
     9841 taccttgatc aacttcgaag acatcagcgc ctcggatttc aacgatgtcc ttttcggaaa
     9901 tgacgccgcc aacctgattt ccggcgcctt cggggatgac caaatatttg gcctgggggg
     9961 caatgacgtt ctcctctcgt cgctcggcaa tgacttggtc gatggtggcg acggtgatga
    10021 cttcatctgg gcagggcaac tggggcaaca accctatgta aacgccggca acgacacgct
    10081 gataggcggg
//
LOCUS       DASVTM010000527         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_421133,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000527 DASVTM010000000
VERSION     DASVTM010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421133"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..523)
                     /locus_tag="VF633_14485"
     CDS             complement(110..523)
                     /locus_tag="VF633_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX8472315.1"
                     /translation="MENRDATIEMTADIVSAYVGNNSVSVTDLPTIIASVHRALTGVS
                     GGVEAPVEQAPKEPAVPVKKSIAPDFLVCLEDGRKFKSLKRHLRTKYNMSPEEYRAKW
                     GLPKDYPMVAPNYAKARSDLAKQMGLGQGGRKAAR"
     gene            complement(711..>1736)
                     /locus_tag="VF633_14490"
     CDS             complement(711..>1736)
                     /locus_tag="VF633_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX8472316.1"
                     /translation="RVAKRLAHLSEIPHSLLRLLICDDLEVARALLEDCPSLPDSDLV
                     RCARDATVEHRRLIASRRNLGELVCEALIEKDEPQVVETLLRNQTASLSQPAIETVVG
                     ASRAHARYVQWLLRRPELRPSHAYVLFWWASAECRRAILQRFAVSREVLQEAAGDVFP
                     MMAAEKWQDPLARKALQFIERRQRNRAAIEKSPYDSLEDAVEAGRPGLTRESVEEISY
                     LAGLKPATGAKIFTDVGGEPLAVLCKATGLPKSALRSMWVGLRRPETGADGSISPHLE
                     HVLIAYDTIAVDRAQTVLRYWNWALSSALTPALMKAIRDGDDDAVDEHSAPQKAAMLV
                     FAAELAR"
BASE COUNT          268 a          553 c          610 g          305 t
ORIGIN      
        1 gacaatggcg aaagcggtgc gtccgggcgt cgcgagggga ccccgccgga acgaaaaacg
       61 cctccccagc gggactgggg aggcgtttcg atcgtcgtgg atcggagtgt cagcgagcgg
      121 ctttacggcc gccttgaccc aggcccatct gctttgcgag atccgaacgc gccttggcgt
      181 agttcggcgc gaccatggga tagtccttgg gcaggcccca cttggcgcgg tattcttcgg
      241 ggctcatgtt gtacttggtg cgcaggtgac gcttgagcga cttgaacttg cggccatcct
      301 ccaggcagac gaggaagtcg ggagcgatcg acttcttcac cggcacggcc ggctccttgg
      361 gggcctgctc gacgggggct tcgacgccgc cggacacgcc ggtcagggcg cggtgcacgc
      421 tggcgatgat ggtggggagg tcggtcaccg acacagagtt gttgccgaca taggccgaca
      481 cgatgtcggc ggtcatctca atcgtcgcgt ctctgttttc catgctcatc tgagctttgc
      541 gtccttcaac agtcacccgg acgactcacg cgtccagttt cgggatcggc aatatgatta
      601 ttcgaagcgg gagacaagtc gcagccgtaa ctttcgcgtg gacgcacgaa gaccctacac
      661 ggtgttcggc tgaacccgcc cgttgtcgcg ctatagagtg cggctgctga ctagcgggcc
      721 agttcggcgg cgaagaccag catggcggcc ttctgcggcg cagaatgctc gtcgaccgcg
      781 tcgtcgtcgc cgtcgcgaat agctttcatc agggcggggg tcagcgccga cgacaacgcc
      841 cagttccagt accgcaggac ggtctgcgcc cgatcgaccg caatggtgtc gtaggcgatc
      901 agcacgtgct ccagatgagg cgatatgctc ccgtccgccc cggtctccgg ccggcggagg
      961 ccgacccaca tcgagcgaag cgccgatttc ggcaggcccg tcgccttgca gaggacggcg
     1021 agcggctcgc cgccgacgtc ggtgaagatc ttggcgccgg tcgccggctt gaggcccgcc
     1081 agatagctga tttcttcgac cgattcgcgg gtcagaccgg gtcggccggc ctccaccgcg
     1141 tcctccaggc tgtcataggg gctcttctcg atggccgcgc ggttccgctg gcggcgttcg
     1201 atgaactgca gcgccttgcg ggcgagggga tcctgccatt tctcggcggc catcatcggg
     1261 aagacgtcgc cggcggcctc ctgcaggact tcccggctga ccgcgaaccg ctgcaggatc
     1321 gcccgccggc actcggcgct cgcccaccag aacagcacgt aggcgtggct cggccggagt
     1381 tcggggcggc gcagcagcca ttgcacgtag cgggcgtgcg cgcgtgaagc gcccaccacc
     1441 gtctcgatgg ccggctggga caggctggcc gtttggttgc gcagcagggt ttcgacgacc
     1501 tgcggctcgt ccttctcgat cagcgcttcg cagacgagtt cgccgaggtt ccggcggctg
     1561 gcgatcaggc ggcggtgctc gaccgtcgcg tcccgggcgc agcggacgag gtcgctgtcg
     1621 ggcagcgacg ggcagtcctc caggagggcg cgggcgacct cgaggtcgtc gcagatcagc
     1681 aagcgaagca ggctgtgggg aatctcggac aggtgcgcca ggcgcttggc gacgcg
//
LOCUS       DASVTM010000528         5305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_421181,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000528 DASVTM010000000
VERSION     DASVTM010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421181"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF633_14495"
     CDS             complement(<1..432)
                     /locus_tag="VF633_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEX8472317.1"
                     /translation="MSGFADLGASLLRKLDPETAHGLAIRALQTIPMPSPKADDPILA
                     TTLASLRLSNPVGLAAGLDKNGEALAGLSKLGFGFVECGSVTPLAQAGNPKPRLFRLS
                     EDRAIINRMGFNNDGAEAVARRLAARGRRPGPVLGVNIGKTK"
     gene            complement(429..773)
                     /locus_tag="VF633_14500"
     CDS             complement(429..773)
                     /locus_tag="VF633_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEX8472318.1"
                     /translation="MSDHPKEAFKIVDAGEWAVAEATGSYAGSAVDLTDGYIHLSAAD
                     QLAGTVRKHYAGRENLLLLTVDLTGLGEALIWEPSRGGALFPHFYGPLPVAAVTATKT
                     FSVGADGAMVFA"
     gene            complement(770..1282)
                     /locus_tag="VF633_14505"
     CDS             complement(770..1282)
                     /locus_tag="VF633_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8472319.1"
                     /translation="MHRLILMRHAKTERAAASGLDRDRALTERGLADAALMGRVLSEK
                     GLRPDLALVSTSTRTRETWDVLQEAFGDVEVQLEPTLYNAPHDVIRRFVEGAEERAGC
                     LLVLAHNPGIHMLAYEYLIESAASPSVMDRMAGGFPTAAAAVFEVDVAGRCVYDGYLV
                     PKAFGGGSDE"
     gene            complement(1292..2080)
                     /locus_tag="VF633_14510"
     CDS             complement(1292..2080)
                     /locus_tag="VF633_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8472320.1"
                     /translation="MCRSIEDLRAFYGEPTGALVRRLIARRLDDAWGEAANCDVLGIG
                     YATPWLDAFVGARRVVAAMPGGQGVELWPAAGRNRTLLVDDRRLPFAAGAFDRILLIH
                     ALEEADDPDLLLTEAVRALAPAGRIILAVAARGGLWARAEATPFGHGRPFTRGQLERL
                     VRSVGLEPTAWSQTLYTPPWRPLLGLADGIEQVGKTIAPGTAGVILLEASRQAYARIR
                     PGGATARVKARPDMVPQPASRSHRSQHEIEPDSGDGMDASPKAP"
     gene            2151..2879
                     /gene="gloB"
                     /locus_tag="VF633_14515"
     CDS             2151..2879
                     /gene="gloB"
                     /locus_tag="VF633_14515"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX8472321.1"
                     /translation="MALTVHLFPCLSDNFGFLIRDEATGAVAAIDTPDADTILAQLEK
                     LGWGRLDLILNTHWHADHTAGNAAIQAATGCEIVGPEEVRRIAPLDRVIKGGDFVRLG
                     ETTFEVTDTPGHTLGHIVYRAVANDIAFVGDTLFPLGCGRLFEGTAEQMWSSLSAIAA
                     WPDETVLYGAHEYTAANARFALSIDDRPEMKAHAEMIFAMRERGEPTEPTTLAIEKRF
                     NPFLRARDATDFAVRRAAKDDFKG"
     gene            complement(2876..3703)
                     /locus_tag="VF633_14520"
     CDS             complement(2876..3703)
                     /locus_tag="VF633_14520"
                     /inference="COORDINATES: protein motif:HMM:NF027577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4908 domain-containing protein"
                     /protein_id="HEX8472322.1"
                     /translation="MKLQRVEKASAPIPAMMAVVAPVASAQFVVIPAVAVLLATMVLA
                     MPTAAEAAQVRSNAQAEQARGMGRGSPRSLPDTGRYVAETGETFIFDRSGSRPLFRFQ
                     SRTETWALTARPAPRGDIIYRNDAGDQILRVTPDGGMTLYSVRAPNGSPASMAGPAPA
                     LARPLLGPVQLSNLMIQRSFMMSRAMGRMISVMLDGEQDEALCTDALIVSTDAVMRMA
                     ASPSLRGQLSGLRAVTIREGRSSSVVYVNGELRVTVQPNQGATGRPSSARIIQAVRR"
     gene            complement(3753..4478)
                     /gene="phbB"
                     /locus_tag="VF633_14525"
     CDS             complement(3753..4478)
                     /gene="phbB"
                     /locus_tag="VF633_14525"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX8472323.1"
                     /translation="MARVALVTGGTRGIGKAIAQRLKEDGMSVAVGYSGNTEAAEATA
                     RELGVMVVKGNVGSFEDCARAVAEVEAELGPIDVLINNAGITRDGFFHKMSHDQWSDV
                     IRVNMDSVFNMTRQVINGMRDRNYGRIVNISSINGQKGQVGQTNYSAAKAGMIGFTKA
                     LALENARKGVTVNCIAPGYIDTEMVGAMDQKVLDSIIAQIPVGRLGKGEEIADMVAWL
                     TGERAGYVTGCTLSLNGGQYLVG"
     gene            complement(4567..>5305)
                     /locus_tag="VF633_14530"
     CDS             complement(4567..>5305)
                     /locus_tag="VF633_14530"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX8472324.1"
                     /translation="GMTGTYGNPDKHSELSGEAITQGGYSDVIVVDQAYVLNIPDSIP
                     LDVAAPLLCAGITTYSPLKHWGIKPGSKVAVVGLGGLGHMAVKQAAAMGAEVTVLSTS
                     DRKKADAERMGAKHFLINSDKDAMRAAAEKFDLIINTVSATHEIASHLQLLAHDGTMV
                     MLGLTTEALPVFAMPLLWRRRSVAGSLIGGIRETQEMLDFCAEHGLASDIEVIAPSQI
                     NEAYARLEKSDVRYRFVIDMSKLDQAA"
BASE COUNT          893 a         1773 c         1734 g          905 t
ORIGIN      
        1 cttggtcttg ccgatgttga cgccgaggac cggacccggc ctgcgtcccc gggcggccag
       61 ccgacgggcg accgcctcgg ccccgtcgtt gttgaagccc atccggttga tgatcgcccg
      121 gtcctcgctg agccggaaca ggcgcggctt cggatttccg gcttgggcca gaggcgtgac
      181 ggagccgcac tcgacgaacc cgaaacccag tttcgacaac ccggccaagg cctcgccatt
      241 cttgtccagt cccgcggcca gcccgactgg attgctcaac cggaggctgg ccagagttgt
      301 cgccaggatc ggatcatcgg ccttgggtga gggcatcggg atcgtctgga gggcccggat
      361 cgccagcccg tgcgccgtct cgggatcaag cttgcgtagc aggctcgcgc ccagatcagc
      421 gaagccgctc acgcgaacac catggccccg tcagccccga ccgagaatgt cttcgtcgca
      481 gtcaccgccg ccaccggcag ggggccgtag aagtgaggga atagcgcccc gccgcgagaa
      541 ggctcccaga tcagagcctc acccagtccg gtcagatcca cggtcagcag cagcaggttc
      601 tcacggcccg cgtagtgttt gcggacggtc ccggccaact ggtcggcggc ggacaggtga
      661 atgtatccgt ccgtgagatc gacggccgat ccggcatacg atcccgtcgc ttcagccacc
      721 gcccactcgc cggcatcgac gatcttgaag gcttccttcg gatggtcgct cattcgtccg
      781 agccgccacc gaaggccttg gggaccagat agccgtcgta gacgcagcga ccggccacat
      841 cgacctcgaa caccgccgcc gctgcggtgg gaaacccgcc cgccatccgg tccatgacgc
      901 tgggcgaagc cgcgctctcg atcagatact cataggcgag catgtgaatg cctggattat
      961 gggccaacac cagcaggcag ccggcccgtt cctccgcgcc ctccacgaag cggcggatca
     1021 catcatgcgg ggcgttgtag agggtgggct cgagctgcac ctcgacatcg ccgaacgcct
     1081 cctgaagcac atcccaggtc tcacgggtac gggtcgatgt cgaaaccaga gccagatcag
     1141 gccggagtcc cttttcggag aggactcgcc ccatcagggc ggcgtcggct aagccgcgct
     1201 ccgtcaacgc ccggtcgcgg tcgagcccgg aggcggccgc gcgctcggtc ttggcgtgtc
     1261 gcatcagaat aagacggtgc atgtttcggc tttagggcgc cttgggcgac gcatccatac
     1321 cgtcgccgga atccggctcg atttcatgtt gtgaccgatg actgcgtgac gccggttgag
     1381 gaaccatgtc cggcctggcc ttgacccgcg ccgtcgcgcc gccgggtcgt atccgcgcat
     1441 aggcctgacg cgaggcttcc agcaggatga cccccgccgt cccgggcgcg atggtcttgc
     1501 cgacctgttc gatgccgtcc gccagaccca gcaagggacg ccagggcggt gtatagagcg
     1561 tctgggacca ggccgtcggc tccagtccta ccgagcgcac cagtcgctcc agttgaccgc
     1621 gcgtaaaggg tcggccatgg ccaaatggcg tcgcctcggc gcgcgcccac aggccgccac
     1681 gggccgccac cgcgaggatg atccgccctg ccggcgccaa ggctcggacg gcctcggtca
     1741 gcagcaggtc cggatcgtcg gcctcctcca gcgcgtggat cagcaggatg cggtcgaaag
     1801 cgcccgcggc gaacggcagg cggcgatcgt cgaccagcaa ggtccggtta cgtccggcgg
     1861 cgggccacaa ctcaaccccc tgcccgcccg gcatggcggc gaccacccgg cgggctccga
     1921 cgaatgcatc gagccagggc gtggcgtaac cgatgccgag aacgtcgcag ttggcggcct
     1981 cgccccaggc gtcgtccaat cgtcgggcga tcaagcgccg taccagcgct ccggtcggct
     2041 cgccgtaaaa ggcgcgaaga tcttcgatgc tgcgacacat gcggcgcact atatgcgttc
     2101 cagcgacgct cgtcgcccac ctcgcttcgc catcctgccg agaccctcac atggccctga
     2161 ccgttcatct gttcccttgc ctgtccgaca acttcggctt cctgatccgc gacgaagcga
     2221 ccggcgccgt tgcggcgatc gacacgcccg acgctgacac gatcctcgcc caactcgaaa
     2281 agctgggctg gggccgactg gacctgatcc tcaatacgca ctggcatgcc gaccacacgg
     2341 ctgggaacgc cgcgattcag gcggcgacgg gctgtgagat cgtgggaccg gaagaggtcc
     2401 gccgcatcgc gccgctggat cgggtgatca agggcggcga cttcgtccga ctaggcgaga
     2461 cgacgttcga agtgacggac acgcccggcc acacgctcgg gcacatcgtc tatcgcgcgg
     2521 tcgcgaatga catcgccttc gtcggcgaca ccctgttccc attgggctgc ggtcggctat
     2581 tcgagggcac cgccgaacaa atgtggtcca gcctctcggc catcgcggca tggcctgacg
     2641 agacggtgct gtacggcgcc catgaataca ccgccgccaa tgcccgcttt gccctgtcga
     2701 tcgacgaccg acctgagatg aaggcccatg ccgagatgat ctttgccatg cgcgagcgcg
     2761 gcgaaccgac cgagcccacg acgttggcga ttgagaagcg gttcaatcct ttcctgcgcg
     2821 cgagggacgc tacggacttc gccgtgaggc gggcggccaa ggacgacttc aagggctagc
     2881 ggcgaacggc ctgaatgatc cgggctgaag agggtcggcc ggtcgcgccc tggttaggct
     2941 ggaccgtgac gcgcagctcg ccgttgacat agacgacaga gctggaccgc ccctctctga
     3001 tggtgacggc gcgcagaccg ctgagctgac cgcgcaggct cggcgaggcg gccatgcgca
     3061 tcaccgcatc cgtcgagacg atcagggcgt ctgtgcacag ggcctcgtcc tgctcgccgt
     3121 ccagcatgac gctgatcatc cggcccatgg cgcggctcat catgaaactg cgctggatca
     3181 tcaggttgga cagttggacc ggcccaagca gaggccgtgc gagcgccggc gccggcccgg
     3241 ccatcgaggc tggcgacccg ttgggcgcac gcacggaata gagggtcatc cccccgtcag
     3301 gcgtgaccct gagaatctgg tcgccggcat cgttgcgata gatgatgtcg ccgcgcgggg
     3361 cgggtctggc ggtcagcgcc caggtctcgg tgcggctctg gaagcggaac aggggccgcg
     3421 agccggaccg gtcgaagatg aaggtttcgc cggtttcggc gacgtaccga ccggtgtcag
     3481 gcaggctccg gggcgatccc cgtcccatgc ctcgggcctg ctcggcctgg gcattgctac
     3541 ggacctgagc cgcctcggcg gccgtcggca tcgccagaac catcgttgcg aggaggaccg
     3601 cgacagctgg aatgacgacg aattgggccg aagcgacggg ggcgacgacc gccatcatcg
     3661 ccggtatcgg cgcagacgcc ttctcgaccc gttgcaactt catgacgcca tcgttgcaag
     3721 cttggcacgg cggattttgg gcgcctccgt gttcagccga ccagatactg gccgccattc
     3781 aggctcagtg tgcagcctgt gacatatcca gcacgttcgc cggtcagcca ggccaccatg
     3841 tcggcgatct cctcaccctt gcccagacgg ccgacgggga tctgggcgat gatcgaatcc
     3901 agcaccttct gatccatggc gccgaccatc tcggtgtcga tatagccggg tgcgatacag
     3961 ttcaccgtca cgcctttgcg agcgttttca agcgccaacg ccttggtgaa gccgatcatc
     4021 ccggccttgg cggcggagta gttggtctgg ccgacctgac ccttctggcc gttgatcgag
     4081 gagatattga cgatgcggcc atagttgcgg tcgcgcatgc cgttgatcac ctgccgcgtc
     4141 atgttgaaca cactgtccat gttgacgcgg atcacgtcgg accattgatc gtggctcatc
     4201 ttgtggaaga agccgtcgcg ggtgatgccc gcattgttga tcaggacgtc gatgggcccg
     4261 agttcggcct cgacctcggc gacggcgcgg gcgcagtctt cgaacgagcc gacgttgccc
     4321 ttgaccacca tgacgccgag ttcgcgcgcc gtggcttcgg cggcttcggt attgccggaa
     4381 tagccgacgg cgacggacat gccgtcttcc ttgagccgtt gcgcgatcgc cttgccgata
     4441 ccgcgcgtgc cgccggtcac cagagccact ctagccattt acgttccctt tcctggccgc
     4501 cggggtcttg cgccccggcc ggtctggaaa agctttaacc tgcgcgaact tgcgcgcaag
     4561 ccggagttac gcagcttgat ccagcttcga catgtcgatg acgaagcggt agcggacgtc
     4621 ggacttctcg aggcgggcat aggcctcgtt gatctgcgac ggggcgatga cctcaatgtc
     4681 cgaggcgagg ccgtgctcgg cgcagaagtc gagcatctcc tgcgtctcgc gaatgccgcc
     4741 gatcagcgaa ccggcgacgc tgcggcgacg ccacagcagc ggcatggcga acaccggcag
     4801 ggcttcggtc gtcaggccca gcatcaccat cgtgccgtcg tgggccagca gttgcaggtg
     4861 gctggcgatc tcgtgcgtcg ccgagacggt gttgatgatc aggtcgaatt tctcggcggc
     4921 cgccctcatc gcatccttgt cggagttgat caggaaatgc ttcgcgccca tgcgctcggc
     4981 gtcggccttc ttgcggtccg aagtcgacag gacggtgacc tcggcgccca tggcagcagc
     5041 ctgtttgacc gccatgtggc cgagaccgcc aagaccgacc accgcgacct tcgagccggg
     5101 cttgatgccc cagtgcttca gcggcgaata ggtggtgatg cccgcgcaca gcaggggcgc
     5161 ggcgacgtcc agcggtatgg agtccgggat gttgagcaca taggcctgat cgacgacgat
     5221 gacgtcggaa tagccgccct gggtgatcgc ctcgccggac agttcgctgt gcttgtctgg
     5281 attgccgtat gtgcctgtca taccg
//
LOCUS       DASVTM010000529         5191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1061505,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000529 DASVTM010000000
VERSION     DASVTM010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061505"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VF633_14535"
     CDS             complement(<1..632)
                     /locus_tag="VF633_14535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX8472325.1"
                     /translation="MIERNWQELIRPEKPQIELGSDAQRKARLVAEPLERGFGVTLGN
                     ALRRVLLSSLQGAAVTAIQIDGVVHEFSSLEGVREDVVDIVLNIKQLALRLHSEGPKR
                     MTLRATGPGQVTAGQIDVPSDIEVLNPNHVICTLDDGASIRMELTVQNGKGYVASEFN
                     RPEDAPIGLIAVDALYSPVKRVAYRVEPTRQGQSMDYDKLVLEVETNGAVS"
     gene            complement(722..1111)
                     /gene="rpsK"
                     /locus_tag="VF633_14540"
     CDS             complement(722..1111)
                     /gene="rpsK"
                     /locus_tag="VF633_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX8472326.1"
                     /translation="MAKEPGRVKKRERKNITSGVAHVNASFNNTMITITDAQGNAISW
                     SSAGHMGFKGSRKSTPYAAQMAAEDAGKKAQEHGVKTLEVNVSGPGSGRESALRALSS
                     VGLTITTIRDVTPMPHNGCRPPKRRRV"
     gene            complement(1121..1489)
                     /gene="rpsM"
                     /locus_tag="VF633_14545"
     CDS             complement(1121..1489)
                     /gene="rpsM"
                     /locus_tag="VF633_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX8472327.1"
                     /translation="MARIAGVNIPTNKRVEIALQYIHGIGPAAAKDIVGKVGIESARR
                     VNQLTDAEVLQIRETIDKDHTVEGDLRRETSMNIKRLMDLACYRGLRHRKGLPVRGQR
                     THTNARTRKGPAKPIAGKKK"
     gene            complement(1618..4089)
                     /locus_tag="VF633_14550"
     CDS             complement(1618..4089)
                     /locus_tag="VF633_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX8472328.1"
                     /translation="MKRTATAMALVLALSGGMANAAWAADPEPKSYAQTIDGLSRQEG
                     LLPVLVDQAGGKVLIQLPAPGADGVMTRVIHHTALRTGVGSAVTGLDRAQIGATNILA
                     FRRIGKRIIAEFENPKYEAPNGSPEEQAAARDAFVGSTVWAGDVVATAPDGSVLVDLS
                     GFLTRDAMGIVETLKATGQGSLKAMPDLTLVDPAATRDFPENLEMEARLTFAVDSPGT
                     ELSRIAPEPRLATFTVRHSFIKLPDNNYQPRDFDPRTGAFATNVTDFSAPLTGAMVHR
                     FANRFRLEKTDPTAARSTVVKPIVFYVDRAAPEPIRSALVDGARWWADAFDKAGYVDA
                     FRVDVMPEGADPLDARYNVINWVNRATRSWSYGQSVVDPRTGEIVKGSVLLGSLRVRQ
                     DMLIFEGLVGADHTGDGLANDPQRIALARMRQLAAHEVGHAIGLQHNFSASTQGRASV
                     MDYPVPKIAITADDQLDFSDAYGVGMGSWDDFAIDWLYSDADAATLDAKAAEGSARLR
                     FANDSDARVGGDAQPWGSLWDNGEDPVVELDHILKIRRIALDRFGLRNLPKGAAVNDL
                     RRRLVPIYLYHRYQVDAVAKLVGGIDYGYPVSGAGGETATPVSVEIQRAALAALGRTL
                     KPAELDLPEPLLALLAAQQSGDGDPQNDIEVFKGQQGRAFDPGVAADVAADVTLDALF
                     APQRVNRIADAGRRDPAALGLGETIDVITTTAFAPAAGRLAEPARRVQAQTVLALAGL
                     LRGDDLSSTSAAIIDDRLQTLATRLKASRAADPVQKAHDRWLGALIGDRERLDQLLEG
                     KHHVPATPPGSPIGGAEEGWHDNDA"
     gene            complement(4175..4915)
                     /locus_tag="VF633_14555"
     CDS             complement(4175..4915)
                     /locus_tag="VF633_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX8472329.1"
                     /translation="MTPTLNRVSRDWLSPNRRRELAVIAGVVLLHTGLFALVALDRVP
                     VAPLDPASPPIDVELVEPLRPPPPPPPPPAPTAGGGAPAAASRVHITPKPPVKPLPDP
                     PPAPIVQAPKPEIVVGVAPTAGLTPGLGLGGQGTGTGTGNGSGNGPGTGDRMGPRLVR
                     GPSPRDIARARPPGARGRGVVAVRCRIRLDRALDQCAVVRETPPGQGFGEAAVSRLMS
                     SFNFLPPTEDGAPVEGQSVTLTIDFPGR"
     gene            complement(5021..>5191)
                     /locus_tag="VF633_14560"
     CDS             complement(5021..>5191)
                     /locus_tag="VF633_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX8472330.1"
                     /translation="GRPDDNPDSFKVRLEAYNRNTAPLLPYYGDEDLLTEVDGMGSIE
                     TVAAAIDAALDN"
BASE COUNT          868 a         1642 c         1791 g          890 t
ORIGIN      
        1 gaaacagcgc cgttggtttc gacttccaac accagcttgt cgtagtccat cgactggccc
       61 tgacgggtcg gctcgacgcg ataggcgacg cgcttgaccg gcgagtagag ggcgtcgacg
      121 gcgatcaggc cgatcggagc gtcttccgga cggttgaact ccgaagcgac atagccctta
      181 ccgttctgga cggtcagttc catgcggatc gaggcgccat catccagggt gcagatgacg
      241 tggttcgggt tcagcacttc gatgtccgaa gggacgtcga tctgaccggc ggtcacctgg
      301 cccgggccgg ttgcgcgcag ggtcatacgc ttggggcctt cggagtgaag gcgcagagcg
      361 agttgcttga tgttcagaac gatgtcgacc acgtcctcgc gcacgccttc cagcgacgag
      421 aattcgtgaa cgacgccgtc gatctggatg gcggtgacgg ccgcgccttg cagcgacgac
      481 agaagcacgc gacggagcgc gttgccgagc gtcacaccga agccgcgctc gaggggctca
      541 gcaaccagac gcgccttgcg ctgggcgtcg gagccgagtt cgatctgcgg cttttcggga
      601 cggatcagct cttgccagtt acgttcgatc ataggtgtcc ctcgaaacga gtgtcgccgg
      661 ttcctctcac ctcgtcaggg aggcggaacc ggcggagaat ggagtcggcg gcggagttcg
      721 cttagacgcg gcggcgcttg ggcggacggc aaccgttgtg cggcatcggc gtgacgtcgc
      781 ggatcgtcgt gatggtcaga ccgaccgaag acagggcgcg cagagccgat tcacggccgg
      841 aacccggacc agaaacgttc acttccagcg tcttcacgcc gtgttcctga gccttcttgc
      901 ctgcgtcttc agcagccatc tgggcggcgt aaggggtcga tttacgcgaa cccttgaagc
      961 ccatgtgacc agccgacgac cacgagatcg cgttgccctg ggcatcggtg atcgtgatca
     1021 tggtgttgtt gaacgaagcg ttcacgtggg cgacgcccga agtgatgttc ttgcgttcgc
     1081 gcttctttac gcgacccggt tccttggcca tgtgtctgtc ttacttcttc ttgccggcga
     1141 tcggcttggc gggaccctta cgggtgcggg cgttggtatg ggtgcgctga ccgcggaccg
     1201 gcaggccctt acggtgacgc aggccgcgat agcaggccag atccatcaga cgcttgatgt
     1261 tcatcgaggt ctcgcgacgc aggtcgccct cgacggtgtg atccttgtcg atcgtttcac
     1321 ggatctgcag gacttcagcg tcagtcagtt ggttcacgcg gcgagcggac tcgatgccca
     1381 ccttgccaac gatgtccttg gcggcggccg ggccgatgcc gtgaatgtac tgcagcgcga
     1441 tttccacgcg cttgttggtc ggaatattga cgccagcgat acgggccacg aagctctcca
     1501 gatacgcgtg aatggacgca gcaaacgctc cggtcaacag accaggagcg gtggcgccct
     1561 tcgcgggaag cgcgccttat acatgtgatt cagattgggt caactcgggc gaagcgttta
     1621 ggcatcgtta tcatgccaac cctcctccgc tccgccgatc gggctaccgg gtggggtcgc
     1681 agggacatga tgcttgcctt caagcaattg gtccagtcgt tcgcgatcgc cgatcagggc
     1741 gccgagccag cgatcatgcg ccttctgcac cggatcagcc gcacgcgagg ccttgagccg
     1801 ggtcgccaac gtctgcagcc ggtcgtcgat gatcgccgcc gaggtgctgg acaggtcgtc
     1861 gccgcgcagc aacccggcaa gcgccaagac ggtctgggcc tgaacccgac gggctggctc
     1921 ggccagccga ccggcggcgg gcgcgaaggc agtcgtggtg atcacgtcga tggtttcacc
     1981 cagtccgagc gcggcaggat cgcggcgacc ggcgtcggcg attcggttga cccgctgagg
     2041 cgcgaacagg gcgtcgagcg tgacgtccgc agcgacgtcg gcggccacgc ccggatcgaa
     2101 ggccctgccc tgctgtccct tgaacacctc gatgtcgttc tggggatcgc catcgcccga
     2161 ttgctgcgcg gccagcaagg ccagcagtgg ttcgggcagg tcgagctcag ccggcttcag
     2221 ggtgcggccc agagccgcga gcgcggcacg ctggatctcg acggacaccg gcgtcgcggt
     2281 ttcgccgcct gcgccggaca ccggatagcc gtagtcgatc ccacccacca gcttggccac
     2341 cgcatccacc tgatagcggt gatacagata gatagggacc agccggcgac gcaggtcgtt
     2401 taccgccgcg cccttgggca ggttgcgcag gccgaagcgg tccagggcga tgcggcggat
     2461 cttcagaatg tgatccagct ccacgaccgg atcctcgccg ttgtcccaca ggctgcccca
     2521 gggctgggcg tcgccgccga cgcgggcgtc cgaatcgttg gcgaagcgca ggcgtgccga
     2581 accctcggcc gctttggcgt cgagcgtcgc cgcatcggcg tcggagtaga gccaatcgat
     2641 ggcgaaatcg tcccacgacc ccatgcccac gccataggcg tcgctgaagt cgagctgatc
     2701 atctgcagtg atcgcgatct tcggcaccgg atagtccatg accgaggcgc ggccttgcgt
     2761 gctggccgag aaattgtgct gcaggccgat ggcgtgaccg acctcgtggg cggcgagctg
     2821 ccgcatccga gccagtgcga tgcgttgcgg atcgttggcc aagccgtcgc cggtgtgatc
     2881 agcgccgacc agcccctcga agatcagcat gtcctgtcgc acgcgcagcg atcccagcag
     2941 gaccgagccc ttgacgatct caccggtgcg cggatcgacc acgctctggc cgtacgacca
     3001 gctgcgcgtc gcgcggttga cccagttgat gacgttgtag cgggcgtcca gcggatcggc
     3061 gccctcgggc atcacgtcca cacgaaaggc gtcgacatag ccggctttat cgaaggcgtc
     3121 cgcccaccag cgcgcgccat ccaccaatgc cgaccggatc ggctcgggcg ccgcgcggtc
     3181 tacgtagaag acgatcggct tgaccacggt cgagcgcgcc gccgtcggat cggtcttctc
     3241 cagccggaac ctgttggcga agcggtggac catagccccg gtcagtgggg ccgagaagtc
     3301 ggtgacattg gtggcgaagg cgccggtgcg ggggtcgaaa tcgcgcggct ggtaattgtt
     3361 gtccggcagt ttgatgaagc tgtggcggac ggtgaaggtc gccagacgtg gctcaggcgc
     3421 aatgcgggac agttcggtgc cgggactatc gaccgcaaag gtcagccggg cctccatctc
     3481 gaggttctcg gggaagtccc gcgtcgccgc aggatcgacg agtgtcagat cgggcatggc
     3541 cttcaggctg ccctgccccg tagccttcaa cgtctcgacg atacccatgg cgtcacgcgt
     3601 caggaagccg gacagatcga ccaacaccga accgtcaggc gcggtggcta cgacatcgcc
     3661 cgcccagacc gtcgagccga cgaaggcatc ccgggcagcg gcctgttcct cgggcgagcc
     3721 gttcggcgcc tcgtatttgg ggttctcgaa ctcggcgatg atccgcttgc cgatgcggcg
     3781 gaaggccaga atgttggtcg cgccgatctg ggcccggtcc agaccggtga cagcggagcc
     3841 gactccggtg cggagggcgg tgtgatggat cacgcgagtc attacgccgt cggcgccggg
     3901 cgctggaagc tggatcagaa ccttgccgcc ggcctgatcg acgaggacgg gcagcagccc
     3961 ctcctgccgg gaaaggccgt cgatggtctg ggcgtatgat ttcggctcag gatcggcggc
     4021 ccaggcggcg ttcgccattc ctccggacag ggccagaaca agggccatcg ccgtcgctgt
     4081 ccgcttcatc ctgaacgcca tgcctgatcc ccgttacgct gcggtgcgat aagggcgtga
     4141 ttggcgggcg ggtgtcaacg cgcggggagc gtcctcagcg tccggggaag tcgatggtca
     4201 gggtaacgct ctgcccttca acaggcgccc catcctcggt cggcggcagg aagttgaacg
     4261 acgacatcag tcgagagacc gccgcctcgc cgaacccctg cccgggcggc gtctccctga
     4321 ccacggcgca ctgatcgagc gcgcgatcca gacggatgcg gcaacgaacg gcgactaccc
     4381 cacggcctct cgctccggga ggacgggcgc gcgctatatc gcgtggcgac ggcccgcgaa
     4441 ccagacgggg tcccatgcgg tcgcctgtcc cgggcccatt gcctgagccg ttgccggtgc
     4501 ccgtacctgt accctgcccg cccagaccga ggccgggtgt cagaccggca gtcggggcaa
     4561 cgccgacgac gatttcgggc ttcggcgcct gcacgatagg agccggcggc ggatccggca
     4621 gcggcttcac cggcggcttg ggcgtgatgt ggacgcgcga cgccgcagcg ggcgcgcctc
     4681 ctcccgcagt cggagcaggt ggaggtggcg gtggaggtgg cgggcggagg ggctcaacca
     4741 gctcgacatc aatggggggt gaggccggat ccagaggcgc aacgggcaca cggtccagcg
     4801 caaccaaggc gaacaggccc gtatgcagca gcacgacgcc tgcgatgacg gcgagctcgc
     4861 gccgtcggtt cggcgaaagc caatcgcggg aaacacggtt tagggtcggc gtcacggggt
     4921 gagttcatcc tgcacgatcg ggaaggaccc taacgcatgg cgcggatgaa cgtcgcctga
     4981 tggcctgatt gtcgagggta gtctcgcctt ggtggaggac ctagttgtcc agcgccgcgt
     5041 cgatggcggc cgcgacggtc tcgatcgagc ccatgccgtc aacctcggtc agcagatcct
     5101 cgtcgccata gtagggcagc aggggcgccg tgttgcggtt gtaggcctcg agccggacct
     5161 tgaagctgtc cggattatca tcggggcggc c
//
LOCUS       DASVTM010000530        12311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_956619,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000530 DASVTM010000000
VERSION     DASVTM010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956619"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..954)
                     /locus_tag="VF633_14565"
     CDS             complement(112..954)
                     /locus_tag="VF633_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8472331.1"
                     /translation="MRAPDWLSRVPDWSIYAIILTAILCLSLSRRENADAPPAPAPPD
                     AEEGALLGPVTPFDPSITVEAPAGPFQPSAGTAFSISADGRWVTARHVVDGCRRPALV
                     VGGGRAVAADLRLARGADIAVLITEGAPPGLQVLDTPELRRGQRAFHPGFPQGRAGEV
                     SSRLLGRETLKVHGRGQRSEPVLAWAEVGRTEGLEGTLAGLSGAPALDRQGRVLGVTI
                     AEAPRRGRIYTSAPDTFSPAIKGLAGAEDIPLDEPITIDNYGRVADTLRRDLRVAQVV
                     CLSS"
     gene            complement(959..1399)
                     /locus_tag="VF633_14570"
     CDS             complement(959..1399)
                     /locus_tag="VF633_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX8472332.1"
                     /translation="MGSTLSNILSSPEIQAFASGFPVMVVHLVATFILLGAGAVIYGL
                     LTPWKEVALIREGNAAASVAFGGVLLGLAIPLAVSLSVSTSLRDIVLWGIATLVLQLL
                     AFRIVDAVLTGLPQRIQEGEVSAAVLLVSAKLATALVLAAALTG"
     gene            1606..2043
                     /locus_tag="VF633_14575"
     CDS             1606..2043
                     /locus_tag="VF633_14575"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX8472333.1"
                     /translation="MTDSILAAPATPDVPTVLVVAVALIDVDGRVLIAKRPEGKQLAG
                     LWEFPGGKVEKGERPEAALIRELREELGIEVAESCLAPFVFASHAYESFHLLMPLYLC
                     RRWSGVVVANEHAALAWVKPNKLSDYPMPPADEPLVAWLRDLL"
     gene            2079..2255
                     /locus_tag="VF633_14580"
     CDS             2079..2255
                     /locus_tag="VF633_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8472334.1"
                     /translation="MLKLINGFLRDQSGATSIEYGLIASLIFLVILASMTAFGSEVSG
                     LFDRASNVVAAATS"
     gene            complement(2271..3146)
                     /locus_tag="VF633_14585"
     CDS             complement(2271..3146)
                     /locus_tag="VF633_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8472335.1"
                     /translation="MTPASGPPVIFDSARRASRLRRSAPHFRNADFLHVRAAMGATDS
                     LEAILRDFPIAVDLSARPEPFADLLAESPAAAKTGTVTVLNADAADPGASALGLEPGS
                     ADLIVSLMTLHWANDLPGALTQIRRALKPDGLFLGTLLGAATLKELRTVLTEAELAER
                     GGAQARVSPFADGYDGAALLQRAGFALPVSDVDRVTVRYPDLFALIRDLRAMGETNQL
                     AGVIRPLTRGILMRAAALYVEKFGDADGRIPATFEIVNLAGWAPHESQQKPLPRGSAK
                     VRMADVFGATEHIGD"
     gene            3196..3981
                     /locus_tag="VF633_14590"
     CDS             3196..3981
                     /locus_tag="VF633_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX8472336.1"
                     /translation="MRPPALRLPHLHIPRLADVARGFADLILPPLAHDSREATAAAGL
                     SADAWSRVIFLEAPVCDGCGAAFEYDGGAFASERCAACLTQPYAFGRARAACVYDEAS
                     RGLILKYKHGDQQQFAGLFARWISRAASDLADQADAVIPVPLHRMRLLSRRFNQAAEI
                     ARPLARSAGLDYLADALIRTERTDTQGGKSARGRRLNVKTAFAVTEAGRRRIKGRRIL
                     LIDDVLTTGATGEACAKALLAAGARAVDLAVIARVRGARILPT"
     gene            4064..4321
                     /gene="grxC"
                     /locus_tag="VF633_14595"
     CDS             4064..4321
                     /gene="grxC"
                     /locus_tag="VF633_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX8472337.1"
                     /translation="MAEVIIYTKPGCPYCSSALALLNKKGAEFTEIVASNDPPKKAEM
                     VEKSGGKATFPQIFIDGKHIGGCDDIQALERKGGLDALLAA"
     gene            4326..5165
                     /locus_tag="VF633_14600"
     CDS             4326..5165
                     /locus_tag="VF633_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8472338.1"
                     /translation="MSAGLDIALIQTRTPATPEAALAHVEPLIREAASGGAKFVLTPE
                     VTNFMEQRRDRKGLLVTDEDQDAAVLGLRHLAAELGVWLLIGSAVAKSGHTGDDRSAN
                     RSLLIDANGGIVARYDKLHVFDVDLANGDTYRESATIRPGDGATVADTPWGRLGMSVC
                     YDVRFPHLYRQLAKAGASMISIPAAFTVPTGEAHWETLLRARAIETGAFILAPAQGGA
                     HEDGRRTWGHSTVVGPWGEVIAKLDHDEPGVLHATLDLTAVARARASVPALLHDREFA
                     APQ"
     gene            5162..5596
                     /locus_tag="VF633_14605"
     CDS             5162..5596
                     /locus_tag="VF633_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX8472339.1"
                     /translation="MIKYALACDQDHPFEAWFGSSSDYDDQAQRGLVECPFCGSHGVA
                     KQIMSPAVAGTRRSSPAPDMAAKMQSMMMDAAREVRAHVEQNFDYVGDSFAKEARAIH
                     EGKSEKREIYGQATPTEVKKLKEDGVPCSPLPAAPPDPAKIN"
     gene            5601..6425
                     /locus_tag="VF633_14610"
     CDS             5601..6425
                     /locus_tag="VF633_14610"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8472340.1"
                     /translation="MGAGAERYDEHEPPKSLRPWIRTIWTYASSEPSGAVQRIAPDGC
                     PELVLDVGSPYEELGDDGVYRRQPPALFAGQMTRPLAMRPVGPIELVAARFEPDGARD
                     WLGRSAHLAVDQRLDMTARLEGVIAPSGDPKAQVAAVVDLLEAERASAGWTVDPEVRA
                     EIDAAQAGQRNDIRTAAQRRALQRRFRERVGISPREFRSILRFRRVFDHIADPEADAV
                     SWLEAGLTAGYFDQPQMARDFQRYLGCTATAWARDQVELALSIASQTYKPPPQSPL"
     gene            6454..6939
                     /locus_tag="VF633_14615"
     CDS             6454..6939
                     /locus_tag="VF633_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX8472341.1"
                     /translation="MILAALTTAALLQTATVTADQLSWISGYWLSCDDEREASETWTG
                     PRGGLMLGSSVTMRDGRLSSFESSRIATVDEAGGDVSYFAGVEGAAPVAFTATEATGT
                     RVVFENVAHDFPNRIVYELVKADDGIEVLNARIEGHIDGRLETMHWSYRKAELNRRCP
                     N"
     gene            6927..7445
                     /locus_tag="VF633_14620"
     CDS             6927..7445
                     /locus_tag="VF633_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8472342.1"
                     /translation="MPELSQDLSWRPMREGDLDRVAELAVLGFPDHFEGRPVFVNRLE
                     LYRRGSFVLAAGDELPVGYLVAYPMRRETAPPLNTLMEVIPKDAEVIYLHDLALDPDV
                     RGGGHTAAIVERLAQQAKADGWPALALVAVNDAAGFWMKRGFTTASLSGMAEKLATYG
                     PDARYMVRPLQA"
     gene            complement(7442..7663)
                     /locus_tag="VF633_14625"
     CDS             complement(7442..7663)
                     /locus_tag="VF633_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472343.1"
                     /translation="MKRLLILAAAASAALLTSACVTVIDAGDDDVAWHSEGGQPFDAA
                     RRDCRAETRHGQQSNAFYACMADKGWTRS"
     gene            complement(7776..8615)
                     /gene="ubiG"
                     /locus_tag="VF633_14630"
     CDS             complement(7776..8615)
                     /gene="ubiG"
                     /locus_tag="VF633_14630"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX8472344.1"
                     /translation="MAASNDTGPPRKPQSDAAFSDADDQIVSGEAFEQASGASIDPAD
                     VARFSAQAALWWDVRGPFAPLHRFNPSRLTFVRDRAAERFGRNTRDRAPFAGLSLIDV
                     GCGGGLLAEPMARMGFDVTAIDASSENIGTARAHAEPQGLDIAYRAATVEQLEAEGAG
                     PFDVVLTMEVIEHVADPKAFIRSCSRLVAPGGMLIVATLNRTLKGLLLGKFAAEYVLR
                     WVPAGTHDWRQFLKPDEIRLMLSEEPVAVAGPFGLALNPLTGRWSESDDVGINYMMIA
                     TRD"
     gene            8670..9971
                     /locus_tag="VF633_14635"
     CDS             8670..9971
                     /locus_tag="VF633_14635"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX8472345.1"
                     /translation="MTRPETTRPDVSASANRLVMKFGGTSMGDLERIRRAARIIAAEV
                     KAGKQVAVVVSAMAGKTNELVAWTDGAGPAAAGLPLSDDEYDVVVASGEQVTAGLLAI
                     TLRNMGLNARTWMGWQIPIITDDAHSRARIEDVPGEVLGAAMDLGEIAVIPGFLGVTT
                     GGRITTLGRGGSDTSAVAVAAALECPCDIYTDVDGVYTTDPRIEKKATKLNRVSYEEM
                     LEMASLGAKVLQTRSVELAMAKQVPLRVLSSFVEPDENGNLPANAGTLICDEEYIVEK
                     RIVSGVTVSRDEARITLLGLADRTDAPADVFTRLAEANVNVDMIVQSQARTEGAVNLT
                     FTTGRRDAVRAADLMKAAQEQIRFEEIRVDADVAKVSVIGVGMRSHAGVAQTMFQALA
                     DKGVKFQAISTSEIKISVLIEDAYAELAVRALHSAYGLDAL"
     gene            10042..>12311
                     /gene="ptsP"
                     /locus_tag="VF633_14640"
     CDS             10042..>12311
                     /gene="ptsP"
                     /locus_tag="VF633_14640"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX8472346.1"
                     /translation="MPIGGGLTTRGPRNLLRQIREAMAGAAPAQERLDRVVRTIAQSM
                     VAEVCSIYLRRASGELELFATEGLNREAVHNTRLMPGEGLVGEVARMAQPISLSDAPS
                     HPSFAYRPETGEDPYHAFLGAPLLRGGRAIGVLVVQNRAERRYDPDEIEDIQTIAMVL
                     AETVASGELLAQDELRDVEVAPHRPERLKGQKFAEGLAFGHVILHEAPIAPEKLLSSD
                     AVLEEMKLNLALADLKNGIDAMLEGGQGKLAGQSFEVLETYRMFADDRGWNRSLVEAV
                     RSGLTAEAAVDRVRNEHRARFAKARDPYIKERLHDFEDLANRLLRVLAGDNPGQRDLP
                     DDAILVARNLGPADLLEYPRDRLKGLLLEEGSAASHAAIVARALGIPCVGRLQGLRDR
                     LSEGDLVIVDGETGEAHLRPRADMLDSVQSRIAVRSQRQAEFAKLRDVPAITADGQRI
                     ILLTNAGLAVDLENLNETGADGIGLFRTEFQFMVSEELPRLNSQTALYKLVLDAAGDR
                     PVTFRTLDIGGDKVLPYMETTEREENPALGRRAIRLGLDRPSLLRLQLRALLAAGAGR
                     ELRVMFPMIATVDEFRAARELVEVECNWARRRGRPLPALLRVGAMIECPSLLWHLDAL
                     LPLTDFVSVGSNDLFQYMYAADRTNPLVSDRYDPLSPPALRALQSIQKACADTGTPVS
                     VCGEMAGRPLEAFALLTLGFTRLSAPAGGVGPVKRMILSADLTAAKRGMASLLGSSAG
                     SIRNEIESLARKLNVIV"
BASE COUNT         2132 a         3970 c         4172 g         2037 t
ORIGIN      
        1 gatgcaggcg gagccatcgc tcacccgttc aacagtcagg gcatgaagcg ggcctggctg
       61 acccccgagg gtgagatcga ggtcgaggtc ttcggggtct agtgcgcggt ctcagctcga
      121 cagacagacg acctgggcca cgcgtaggtc tcgcctgagg gtgtcggcga cgcggccgta
      181 gttgtcgatc gtgatcggct cgtccagcgg tatgtcttcg gccccggcca gacccttgat
      241 cgcggggctg aaggtatcgg gcgcggaggt gtaaatccgg ccacggcggg gcgcttcggc
      301 aatggtgacg ccgagcacgc gcccctgacg atccagcgcc ggggctccgg acagcccggc
      361 gagggtgccc tcaagccctt ccgtgcgacc gacttcggcc caggcgagga ctggttcgct
      421 tctttggccg cggccgtgaa ccttgagggt ttcgcggccg agcagcctgc tggacacctc
      481 gccggcacgg ccttgcggaa agcccggatg gaaggcgcgc tggccacgcc gtagctccgg
      541 tgtatcgagc acctgaagtc ccggaggggc gccttcggta atcagcacag cgatgtcggc
      601 gccgcgcgcc agacgcaggt cggccgcgac cgcccgacct ccaccgacaa cgagcgctgg
      661 acgtcggcaa ccatcgacca cgtgccgggc cgtgacccag cgaccatcgg cggagatgga
      721 aaaggcggta cccgccgagg gctggaacgg cccagccggc gcttccaccg tgatcgaagg
      781 gtcgaagggc gtgacgggtc ccagcagcgc gccctcctcg gcgtcagggg gtgcgggagc
      841 tggtggggcg tcagcgtttt cacgtcggct cagcgacaag cacaggatcg cggtgagaat
      901 gatggcgtag atgctccagt cgggcaccct ggaaagccag tcgggcgccc gcaccggctc
      961 agcccgtcag ggcggcggcg agaacgagcg cggtcgccag cttggccgat accagcagga
     1021 ccgcggcgga aacttcgcct tcctgaatcc gctgtggaag gccggtcaag actgcgtcga
     1081 cgatgcggaa cgctagcagt tgcagcacca gcgtggcgat gccccagagc acgatgtccc
     1141 tgagggaggt cgagaccgag agcgacaccg ccagcggaat ggccagaccc agcagcacgc
     1201 cgccgaaggc cacgctggct gcggcattgc cctcgcggat cagggcgacc tctttccacg
     1261 gggtcagcag gccgtagatc accgctcccg cacccagcag gatgaaggtc gcgaccagat
     1321 gcaccaccat gaccgggaag ccgctggcga aggcctgaat ttcgggactg gagaggatgt
     1381 tggaaagggt cgagcccatg gtctggaata atctggacga acgcttgcgc gtggagcccg
     1441 gccggggcct cggtgtcaca ggccttgccc gaatcctgcc ccaatccgca agcgcgccag
     1501 cgccacactg tgtgacgaaa cattacagtc caagggcccg tggactcatg aataaggggt
     1561 ccggtcgatc aagacgctaa aaacagagcg gtgtagagcg gggagatgac cgattccatt
     1621 ctagccgccc ccgctactcc cgatgttccg acagtcctcg tcgtcgctgt tgcattgatt
     1681 gatgtcgacg gacgggtgct gatcgcaaaa cgaccagagg gcaagcaatt ggccggctta
     1741 tgggagtttc cgggcggcaa ggtggagaag ggcgagcgac cggaggcggc gctcatccgc
     1801 gaacttcgcg aggaactggg gatcgaggtc gccgaaagct gcctggcccc gttcgtgttc
     1861 gccagccacg cttacgaaag ctttcacctt ctgatgccat tgtacctgtg tcggcgctgg
     1921 tccggcgtgg tcgtcgccaa cgaacacgcc gcattggcgt gggtgaagcc gaacaagctg
     1981 tcggattatc ccatgccccc cgccgatgag ccgctggtcg cctggttgcg cgaccttctt
     2041 tgagaccccg cccgacggcg agcttgcgag gagatccgat gcttaagctg attaacggtt
     2101 tcttgcgtga ccagtccggc gcgacctcga tcgaatacgg actgatcgcc tccctgatct
     2161 ttctggtgat cctcgcttcg atgaccgcgt tcggaagcga ggtgagcgga ctgttcgaca
     2221 gggcgtcaaa cgtggtcgcc gcagcgacca gttgacaggc ctcacaggga tcagtcgccg
     2281 atgtgttcgg tcgcgccgaa gacatccgcc atgcggacct tggccgagcc gcgggggagg
     2341 ggcttttgtt ggctctcatg cggagcccag ccggccagat tgacgatctc gaacgtcgca
     2401 ggaatgcgcc cgtcagcatc cccgaacttt tcgacataga gcgccgccgc ccgcatcagg
     2461 atgccacgtg tcaggggacg gatgaccccc gcgagctgat tggtctcgcc catggcgcgt
     2521 agatcgcgga tcaaggcgaa caggtcggga tagcgcaccg tgacccggtc tacgtccgag
     2581 accggcaagg cgaatcccgc tcgttgcagc aaggctgcgc cgtcgtaccc gtcggcgaag
     2641 ggcgagacgc gggcctgcgc cccgccgcgc tcggccagtt cagcctcggt caggaccgtg
     2701 cgcagttcct tgagggtcgc ggcccctagc agcgtgccga gaaacaagcc gtcgggcttc
     2761 agggcgcgcc ggatctgggt cagggcgccg ggcaggtcat tggcccagtg cagcgtcata
     2821 agagagacga tcaggtccgc cgagccgggt tcgaggccga gcgccgatgc tccggggtcc
     2881 gcggcgtcgg cattcaggac cgtgactgtg ccggtctttg ccgcagcggg gctttctgcc
     2941 agcaggtcgg cgaagggctc gggccgcgca gaaagatcga cggcgatggg aaagtcgcgc
     3001 aggatcgcct caaggctgtc agtcgcgccc attgcagccc gcacgtgcag gaagtcggca
     3061 ttgcggaaat gcggcgccga tcgcctcaag cgtgaggcgc ggcgggcgct gtcgaagatt
     3121 acgggaggac cggaagcggg tgtcatgaat gatcaggatg gggcttcgcg agaccgattg
     3181 gaagacgatg cggctatgcg accgcctgcg cttcgccttc cccacctgca cattccccga
     3241 ttggccgacg ttgcgagggg cttcgccgac ctgatcctgc cgcctctggc ccatgacagt
     3301 cgcgaagcga cggcggctgc ggggttgagc gccgacgcct ggagccgggt gatcttcctc
     3361 gaggccccgg tgtgtgacgg ctgcggggcg gcgttcgaat acgacggcgg cgctttcgcc
     3421 tctgagcgtt gcgccgcctg tctgacccag ccctatgcct tcgggcgtgc gcgggcggcc
     3481 tgcgtctatg acgaggcgtc gcgcgggctc atcctgaaat acaagcacgg cgaccaacag
     3541 cagttcgctg gcctttttgc gcgctggatc agccgcgcgg cctctgacct cgcggatcag
     3601 gccgacgccg tcatccccgt gccgctgcac cggatgcgat tattgtcgcg gcgcttcaat
     3661 caggccgccg agatcgcccg tccgctggct agatcagcgg ggctcgacta tctggccgac
     3721 gccctgatcc ggaccgagcg aacggacacc caaggcggca aaagcgcgcg cgggcggcgg
     3781 ctgaacgtca agacagcgtt tgccgtgacc gaggccgggc gacgtcgcat caagggccga
     3841 cgcatcctgc tgatcgacga cgtcctgacc accggcgcga cgggcgaggc ctgcgcgaag
     3901 gcgctgctgg cggcgggggc tcgagcggtc gatctggcgg tcatcgcccg ggtgcgcggg
     3961 gctcgaattc ttcctacata ggaacatccg atccgatcag agcctttcga tcaggcgcgg
     4021 tttcgcgcta ggtccgatca acacattgta ctggagtgct cgcatggccg aggtcatcat
     4081 ctacaccaaa cccggttgcc cctactgctc gtccgcgctg gcgctgctga acaagaaggg
     4141 cgccgaattc accgagatcg tcgcctccaa cgatccgccg aagaaggccg agatggtcga
     4201 gaagtcgggc ggcaaggcta ccttcccgca aatcttcatc gacggaaagc acatcggcgg
     4261 ctgcgacgac atccaagcgc tcgagcgcaa gggcggactc gacgcgctgc tggccgcctg
     4321 atctcatgag cgcagggctc gacatcgccc tgatccagac gcggactccg gcgacgccgg
     4381 aggccgcgct ggcccatgtc gagccgctca tccgcgaggc ggcttccgga ggggcgaaat
     4441 tcgtcctgac tccagaagtg acaaacttca tggagcagcg acgcgaccgg aagggcctgc
     4501 tggtcaccga cgaggatcag gacgccgccg ttctcggcct gcggcacttg gcggcggagc
     4561 tcggcgtctg gttgctgatc ggttcggcgg tggcgaagtc ggggcatacg ggcgacgacc
     4621 ggtcggcgaa tcgctcgctg ctgatcgatg ccaacggagg catcgtcgcc cgctacgaca
     4681 agctccacgt gttcgacgtc gatctggcca atggcgacac ctaccgcgag agcgccacca
     4741 ttcgtcccgg cgatggcgcg accgtggccg atacgccgtg gggccggctg gggatgagcg
     4801 tctgctacga cgtgcgcttt ccgcatctgt atcgccagct ggcaaaggcc ggcgcatcga
     4861 tgatctcgat ccccgccgcc ttcaccgtgc ccaccggcga ggcgcactgg gagaccctgc
     4921 tgcgggcccg cgccatcgag acgggggcat tcatccttgc gccggctcag ggcggagcgc
     4981 acgaggatgg ccgccgaacc tggggtcatt cgaccgtcgt cggcccatgg ggtgaagtga
     5041 tcgcaaagct ggaccacgac gaacccggcg ttctgcacgc gacgctcgac ctgaccgccg
     5101 tcgcgcgcgc gcgggcctcg gtgccggccc tgctgcacga ccgcgagttc gcagcgcccc
     5161 aatgataaaa tacgctctcg cctgtgatca ggaccacccg ttcgaggcgt ggttcgggtc
     5221 ctcctccgac tatgacgatc aggcgcagcg gggtctggtc gaatgcccgt tctgtggttc
     5281 gcacggcgta gccaaacaga tcatgtcgcc agccgtcgcc ggcacgagac ggtcctcgcc
     5341 cgccccggac atggcggcga agatgcagtc gatgatgatg gacgccgccc gcgaagtccg
     5401 cgcccacgtc gagcagaatt tcgactacgt cggcgacagc ttcgccaaag aggcccgcgc
     5461 catccacgag ggcaagtccg agaagcgtga gatctacggt caggccaccc cgaccgaggt
     5521 caagaagctc aaggaggacg gtgtgccctg ttcgcccctg cccgccgctc ctcccgatcc
     5581 cgccaagatc aactgaccgg atgggcgcag gggcggaacg atacgatgag catgagccgc
     5641 cgaagtccct gcggccatgg atccgaacca tctggaccta tgcctcgtcc gagccttccg
     5701 gagcggtgca gcggatcgcc ccggacgggt gccccgaact tgttctcgat gtcggcagtc
     5761 cgtacgagga acttggcgac gacggcgtct atcgtcgtca gcctcctgct ctgtttgcag
     5821 gacagatgac ccgaccgctg gccatgaggc ctgtcggacc gatcgagctg gtcgccgcgc
     5881 ggttcgaacc cgacggcgcc cgcgactggc tgggtcggtc agcgcatctg gcggtcgatc
     5941 agcgcctcga catgactgca cgtctggaag gtgtcattgc gccgtctggc gatccgaagg
     6001 cgcaggtcgc cgctgtggtc gatctgctgg aggccgaaag ggcgagcgcc ggttggaccg
     6061 tcgatcccga ggtcagggcc gagatcgacg ccgctcaggc cgggcaacgg aatgatatcc
     6121 gcaccgccgc ccaacggcgc gccctgcagc ggcggttccg ggaacgggtc gggatttcgc
     6181 cacgcgagtt ccgctcgatc ctgagattcc gccgcgtgtt cgatcatatc gcggatccag
     6241 aggccgacgc ggtgtcgtgg ctcgaagcgg gtctgaccgc aggctatttc gaccagccgc
     6301 agatggcgcg cgacttccag cgttacctcg gctgcaccgc cacggcttgg gcacgagatc
     6361 aggtcgaact ggcgctcagc atagcgtcgc aaacctacaa gcctccgccc cagtctccgc
     6421 tctaggatcg cgttacatcc agtggagccc gacatgatcc tcgcagcctt gaccaccgca
     6481 gccctgttgc agaccgcgac cgtgaccgcc gatcagctgt cgtggatcag tggctactgg
     6541 ttgtcgtgcg acgacgagcg ggaggcgtcc gagacctgga ccggtccgcg cggcggcctg
     6601 atgctggggt cgtccgtgac gatgcgagac ggccggctca gcagcttcga gtcgagccgt
     6661 atcgcgaccg tcgatgaggc gggtggcgac gtctcctatt tcgccggtgt cgagggcgcc
     6721 gcccctgtcg ccttcaccgc aacggaggcg actggaacgc gcgtcgtgtt cgagaacgtc
     6781 gcccacgact tccccaatcg catcgtctat gaattggtca aagctgatga cggcattgag
     6841 gttctcaatg cccggatcga aggccacatc gacggacgcc tcgagacgat gcactggtcg
     6901 tatcgaaaag ccgagttgaa caggcgatgc ccgaactgag ccaggatctg agctggagac
     6961 cgatgcgcga aggcgatctt gatcgggtcg ccgagctcgc cgtgcttggc tttccggatc
     7021 atttcgaagg ccgccccgtg ttcgtcaatc ggctggaact gtatcggcgc gggtcgttcg
     7081 tgctggcggc cggggatgag ctgccggttg gttatctggt cgcttatccc atgcgcaggg
     7141 agaccgcgcc gccgttgaac accttgatgg aagtgatccc gaaagatgcg gaggtgatct
     7201 atctgcacga cctggccctg gatcccgatg ttcggggcgg agggcatacg gcggcaatcg
     7261 tcgaacggct ggcccaacag gcgaaggcgg acggatggcc cgccctcgct ctcgttgcgg
     7321 tcaatgacgc cgccggcttc tggatgaagc gaggtttcac caccgcgtcc ttgtctggca
     7381 tggcggagaa attggccacc tacgggccgg acgcacgata catggtccgg ccactccaag
     7441 cctagctgcg ggtccagcct ttgtcggcca tgcacgcgta gaaggcgttg gactgttggc
     7501 catgacgggt ctcggcgcgg cagtcgcgac gagcggcgtc gaagggctgg ccgccctcgc
     7561 tgtgccaggc gacgtcatca tcgccggcgt cgatgacggt gacacaggcc gaggtgagca
     7621 gggcggcaga agcggcggcg gcgaggatca gaaggcgttt catcttgggc tccagggttt
     7681 cgataacccc gtcatcccac cgccctgtgg ggaaatcccg ttacaagaca gacaggtgga
     7741 tgaaatcgcg gtaagctttg ccgcaaactc gccggtcagt cgcgggtggc gatcatcatg
     7801 tagttgatgc cgacgtcgtc gctttcgctc cagcggccgg tcaggggatt gagcgccaga
     7861 ccgaatgggc cggcgaccgc gaccggttcc tcggacagca tcagtcggat ctcgtcaggc
     7921 ttgaggaact ggcgccagtc gtgggtgccg gcagggaccc agcggagcac atattccgct
     7981 gcgaacttgc cgagcagcag acccttgagc gtgcggttca gcgtggccac gatcagcatt
     8041 ccgccgggcg cgaccagccg tgagcaggac cggatgaagg ctttgggatc ggcgacgtgt
     8101 tcgatcacct ccatggtcag gacgacgtcg aaggggcctg cgccttccgc ctcgagctgt
     8161 tccacggtgg ctgcgcgata ggcgatgtcg aggccctgcg gttcggcatg ggcgcgggca
     8221 gtgccgatgt tctcggacga ggcatcgatg gcggtgacgt cgaaacccat ccgcgccatc
     8281 ggttcggcca gcagtccgcc gccacagccg acgtcgatca ggctgagacc cgcgaacggc
     8341 gcacggtctc gcgtattgcg tccgaaccgc tcggcggccc ggtcgcgcac gaaggtcagg
     8401 cggctgggat tgaaccggtg caggggcgcg aacgggccgc gtacgtccca ccacagggcc
     8461 gcctgggccg agaagcgtgc gacatcggcc ggatcgatcg acgcgccgga cgcctgctcg
     8521 aaagcttcac cggagacgat ctgatcgtcg gcgtcagaaa aagccgcatc gctttggggt
     8581 ttgcgggggg gtccggtgtc gttagaagcg gccatcccgg aggcatgaac cgccgggcga
     8641 tttcttgcaa gatgggggcg accgccagga tgacgcggcc cgagaccaca cgacccgacg
     8701 tatcagcatc cgccaaccgg ctggtgatga aattcggcgg cacctcgatg ggcgacctcg
     8761 agcgcatccg tcgcgccgcg cgtatcatcg ccgccgaggt caaggccggc aaacaggtcg
     8821 ccgttgtcgt ctcggccatg gcaggcaaaa ccaatgagct ggtcgcgtgg accgatggcg
     8881 cgggccccgc tgccgccgga ctgccgctgt cggacgatga atatgatgtc gtcgtcgcct
     8941 cgggagaaca ggtcacggcc ggtctgctgg cgatcaccct gcgcaacatg ggtctgaatg
     9001 cgcggacatg gatgggctgg cagatcccga tcatcaccga cgacgcgcac agccgcgccc
     9061 gcatcgaaga cgtgccgggc gaggttctgg gcgccgcgat ggatctgggc gagatcgccg
     9121 tcatccccgg ctttctgggc gtgaccaccg gcggccgcat caccactctc ggccgtggcg
     9181 gctccgacac ctctgccgtg gccgtggccg cggcgctgga atgcccatgc gacatctaca
     9241 ccgacgtaga cggcgtctac acgaccgacc cgcgcatcga gaagaaagcc accaagctga
     9301 accgcgtctc ctacgaggag atgctggaga tggcgtcgct gggcgccaag gtgctccaga
     9361 cgcggtcagt cgaactggcc atggccaaac aggtgccctt gcgggtgctg tcgagcttcg
     9421 tcgaacccga cgagaatgga aatctcccgg ccaacgccgg caccctgatc tgtgacgagg
     9481 aatacatcgt ggaaaagcgc atcgtatcgg gcgtgaccgt cagccgcgac gaggcccgca
     9541 tcaccctgct gggtttggcg gaccgcaccg acgccccggc ggacgtgttc acccgtctgg
     9601 ccgaggccaa cgtcaacgtg gacatgatcg ttcagtcgca ggcccgcacg gagggcgcgg
     9661 tcaacctgac cttcacaaca ggtcgtcgtg acgccgttcg cgccgccgat ctgatgaagg
     9721 ctgcgcagga gcagatcagg ttcgaggaaa tccgcgtcga cgcggacgtg gccaaggtct
     9781 cggtcatcgg cgtcggcatg cgcagccacg ccggtgtcgc ccagaccatg ttccaggccc
     9841 tcgccgacaa gggcgtcaag tttcaggcga tctcgacatc cgaaatcaag atcagcgtcc
     9901 tgatcgaaga cgcctatgcg gaactcgcgg ttcgtgcgct tcattcggca tacggactgg
     9961 atgcgctata ggtagcgtca cagtggatcc ctatctgggg tagccttacg ctaactaggt
    10021 agacacgggc tgggaggctg gatgccaata ggcggcggat tgacgacgag ggggccgcgc
    10081 aacctcctgc gccagatccg cgaggcgatg gccggggcgg ctccggctca ggagcggctg
    10141 gaccgtgtcg tacgcacaat cgcccagtcg atggtggccg aggtctgctc catctatctg
    10201 cgccgcgcct ccggcgagct cgaactgttc gcaaccgaag gcctgaaccg cgaggccgtg
    10261 cacaacaccc gcctgatgcc cggcgagggt ctggtcggcg aggtggcgcg gatggcccag
    10321 ccgatcagcc tgtccgacgc cccgtcgcac cccagcttcg cctatcgtcc ggaaaccggg
    10381 gaagatccct atcacgcctt cctcggtgcg cccttgctgc gcgggggtcg ggcgatcggc
    10441 gtgctggttg ttcagaaccg ggctgagcgt cgctatgatc cggacgagat cgaggacatc
    10501 cagaccatcg cgatggtgct ggccgagact gtggcctcgg gcgaactgct cgcgcaggac
    10561 gaactgcgcg atgtcgaggt cgccccccac cgtccggaac gcctgaaggg ccagaaattc
    10621 gccgaaggtc tcgccttcgg tcatgtgatc ctccacgagg cgccgatcgc gcccgaaaag
    10681 ctgctgtcct cggacgccgt ccttgaggaa atgaagctca acctcgcgtt ggccgacctc
    10741 aagaacggca tcgacgccat gctcgagggt ggtcagggca agctggccgg tcagtcgttc
    10801 gaggttctgg aaacctaccg gatgttcgcc gatgaccggg ggtggaaccg gtcgctggtc
    10861 gaggctgtgc gctccggctt gaccgccgag gctgctgtgg accgggtgcg caacgaacac
    10921 cgcgcccgct tcgccaaagc tcgtgatccg tacatcaagg agcggctgca cgacttcgag
    10981 gatttggcca accggctgct gcgggttctg gcgggtgata acccgggcca gcgcgacctg
    11041 cccgacgacg ccattctggt cgcccgcaat cttggcccag cagacctgct ggaatatccg
    11101 cgcgaccggc tgaagggcct gttgctggaa gaaggctcgg cggccagcca cgccgccatc
    11161 gtggcccgcg cgctgggcat tccatgcgtc ggtcgtctgc aaggattgcg cgatcggctg
    11221 agtgaaggcg acctcgtcat cgtcgatggc gagaccggcg aggcgcatct gcgcccccgg
    11281 gccgacatgc tggactcggt ccagtcgcgc atcgccgtcc gcagccagcg tcaggccgag
    11341 tttgcaaagc tgcgcgacgt tccggccatc accgccgacg gccagcgcat catactgctg
    11401 accaacgccg gcctcgccgt cgacctggaa aacctgaacg agacaggagc cgacggcatc
    11461 ggcctgttcc gcaccgagtt ccagttcatg gtctcggaag agctgccgcg cctgaacagc
    11521 cagacggcgc tgtacaagct ggttctggac gcggcaggcg accggcctgt gaccttccgc
    11581 accctcgata tcggcggcga caaggtcctg ccgtacatgg agaccaccga gcgggaggaa
    11641 aatccggcgc tgggtcgtcg cgccattcgc ctcggcctgg accggccgtc actgctgcgt
    11701 ctgcaactcc gtgcgctgct ggccgccggc gccgggcgtg agttgcgcgt gatgttcccg
    11761 atgatcgcca ccgtcgacga gttccgggcg gcgcgggagc tggtcgaggt cgaatgtaac
    11821 tgggcccgga gacgcggtcg tccgctgccc gccctgctgc gggtcggcgc gatgatcgaa
    11881 tgcccaagcc tgctctggca cctggacgcc ctgttacccc tgaccgactt cgtctcggtc
    11941 ggatccaacg acctgttcca gtatatgtac gccgcagacc ggaccaatcc gcttgtctcg
    12001 gaccgttacg acccgttgtc gcccccagcc ctccgggcgt tgcagtcgat ccagaaggct
    12061 tgcgccgaca caggcacgcc cgtctccgtc tgtggcgaga tggctggacg gccgctggag
    12121 gctttcgccc tgctgacact cgggtttacc cggctgtcgg ctccggccgg cggggtcggt
    12181 ccggtgaagc gaatgatcct gtccgccgac ctgacggcgg ccaagcgggg catggccagc
    12241 cttctgggca gttccgcggg ttcgatccgc aatgagatcg aaagtctggc ccggaagttg
    12301 aacgtcatcg t
//
LOCUS       DASVTM010000531         4626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_690455,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000531 DASVTM010000000
VERSION     DASVTM010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690455"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VF633_14645"
     CDS             <1..503
                     /locus_tag="VF633_14645"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEX8472347.1"
                     /translation="ILGYGFNPLTVFFCHDREERLMAILYEVSNTFGQRHSYLMPVAI
                     GDGCVRQSVAKQFHVSPFMDMDLIYRFSVIPPTGAGEETAAVNIIVEDGEGPMLLTGF
                     SGRRKPLTDRNLMLAWLAHPLLTLQVIAGIHWEAFLLIRKGLRPRSGRVPSAPVTVGR
                     AVAAIN"
     gene            505..1050
                     /locus_tag="VF633_14650"
     CDS             505..1050
                     /locus_tag="VF633_14650"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX8472348.1"
                     /translation="MNTMLTFRTAVMALSVAVAAPAMIPATAFAEAPEPRSHVDLDRF
                     MGRWYEILRTPNDHQRNCWAASQVWARRGPDRFSIAQTCHRGSRDGRPTTVNTSARVL
                     DQTTNAKFEASFFGGLIRQRYWVIEVAPDYSWMIATTADGQFPALLSRSPTMSAAEQI
                     RLRARIAQLGMPTRQLEPSGR"
     gene            1052..1870
                     /locus_tag="VF633_14655"
     CDS             1052..1870
                     /locus_tag="VF633_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX8472349.1"
                     /translation="MMTALLVALFLMATVMTTAWAVSLKTDNTGWIDVFWTFGTGLTG
                     AVVALTPWAGEPSKRQMLVALLVAVWSVRLGSYIALRVAGGVEDSRYLMMKEEWGSGF
                     RKNLFSLAIVQAPATTLLCASIAAAALSPGEDLGLRDWIGAAILLIAIVGEGVADRQM
                     KRFRADRSNHGKVMDKGLWGLSRHPNYVFEWFGWVAYPVIAIDLSGAWPQGWASLIAP
                     VVMYLILTRLTGVPPLEKTMMKSRGKVYADYQNRVGAFFPRRAHKPTKSAGTAR"
     gene            1867..2895
                     /locus_tag="VF633_14660"
     CDS             1867..2895
                     /locus_tag="VF633_14660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8472350.1"
                     /translation="MSLISNSLNKLQDAPFPDFITRPAIALLVANARKSLKAPPVDIE
                     VTFAREMAERPVAEHTDAANQQHYELPARFFELCLGKHRKYSSCFYESEDATLDEAEA
                     SALRITCEHADIQDGQSVLELGCGWGSLSLCMAERYPNANITTVSNSRSQKASIDDTA
                     RRRGLTNLTIITADMNDFTLDQTFDRIVSVEMFEHMANWRALLGKARGWLKPEGRMFI
                     HVFSHIDAPYRFEVSDQSDFIAQHFFTGGVMPSHGLMKQYGDLLTVEDEWRWSGTHYQ
                     RTADDWLDNFDKHEAEIRGLMVEVYGLENAPLWMRRWRRFFMATAGLFGDSDGSVWGV
                     SHYRLKRA"
     gene            2898..3302
                     /locus_tag="VF633_14665"
     CDS             2898..3302
                     /locus_tag="VF633_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HEX8472351.1"
                     /translation="MMKFIAAYGCSALVVAIMDGVWLTMMGDKLYRPVLGDFMAKSVN
                     IPAAIAFYFIYLAGLTFLATQPALQQLSVGKAVVNAAVFGFVAYATYDLTNQATLARW
                     STTITLADMAWGTVMSVFAAVAGYYGSRLLVR"
     gene            3476..>4626
                     /locus_tag="VF633_14670"
     CDS             3476..>4626
                     /locus_tag="VF633_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX8472352.1"
                     /translation="MKPSIRAIREPWAAWNDLRRPVWLFDPVALRGLYANDAALALWG
                     AASLEELLARDFSILSPAVRTRTQRLAWATAGGEEVSERWTFYPNGQPVTVQAVISTY
                     SLPDSTSAALLFEAAPVDAEAEERRALEALRHTSTAISLFDQEGRPLFANPAAFAAYG
                     RSDLPLASRFVEPERAAQLLSLAMSGETATDICQVTTESGVRWHHLDARSGLDPVTGS
                     ATLLLNEHDITDTVEAQTARALAERKTAESEARQSFLTEMSHELRTPLNAVIGFADLL
                     SRAQLAEEHVEQAHSILEGARRLATVVNEMIRIADGTTAPPVSVTVPTTPRSPSAAEE
                     RATRVLYVDDNAANRRLVVTVLGSQGFVCETAADGAEGVEAAKTGDWDV"
BASE COUNT          800 a         1514 c         1436 g          876 t
ORIGIN      
        1 gcatcctcgg ttacggcttc aatccgctca ctgtcttctt ctgccatgac cgcgaggaac
       61 ggctaatggc cattctgtac gaggtgtcca acacctttgg tcagcggcat tcctatctga
      121 tgccggtcgc catcggtgac ggctgcgtgc gtcagtcggt ggcgaaacag ttccacgtct
      181 cgccgttcat ggacatggac ctgatctacc gcttctccgt cattcccccg acaggcgccg
      241 gggaagagac cgccgccgtg aatatcatcg tcgaggacgg ggaggggccg atgctgctga
      301 ccggcttctc cggccgtcgc aaaccgctca cggaccgcaa tctgatgctg gcgtggctgg
      361 cgcatccctt gctgaccttg caggtcatcg ccggcatcca ctgggaagcc ttcctgttga
      421 tccggaaggg tcttcgcccg cgttcgggcc gcgtgccctc cgctcccgtc acggtcggcc
      481 gcgcggtcgc ggcgataaat tgagatgaac acgatgctga cgttccgcac cgccgtcatg
      541 gccctttctg tcgccgtcgc ggctcccgcg atgatccccg cgacagcctt cgctgaggcc
      601 cccgaaccgc gcagccacgt cgatctggat cgcttcatgg gccgctggta cgagatcctg
      661 cgcacgccca acgaccatca gcgcaattgc tgggcggcga gtcaggtgtg ggcccggcgc
      721 ggacccgacc gcttctccat cgcacagacc tgccatcggg ggtcgcgcga cgggcgaccg
      781 acgacggtca acaccagcgc ccgggttctg gatcagacga ccaacgcaaa gttcgaagcc
      841 agcttcttcg gcggcctcat ccgtcagcgc tactgggtga tcgaggtcgc gccggactac
      901 agctggatga tcgccaccac cgccgacggt cagttcccgg cgctgctgtc acggagtccg
      961 acaatgtctg cagccgagca gatccggctt cgcgcccgca tcgctcaact gggcatgccg
     1021 acccgtcagc tcgaaccctc ggggcgttga gatgatgacc gcgctcctcg tcgccctgtt
     1081 cctgatggcg acggtgatga ccacagcctg ggccgtttcg ctaaagaccg acaacaccgg
     1141 atggatcgac gtcttctgga ccttcggcac cggactgacc ggcgcggtcg tcgccctcac
     1201 gccttgggcg ggggagccgt cgaagcgcca gatgctcgtc gcccttctgg tggcggtctg
     1261 gtccgttcgg ctgggatcct acatcgcact ccgcgtggcg gggggcgtcg aggactcccg
     1321 ctatctgatg atgaaggaag agtggggctc gggcttcagg aaaaacctct tcagcctcgc
     1381 tatcgttcag gcgcccgcaa cgacactttt gtgcgcttcc atcgccgctg cggctttgag
     1441 tccgggggag gatctcggcc tgcgtgactg gatcggcgcc gccatcctcc tgatcgccat
     1501 cgtcggagag ggcgtcgccg accgccagat gaagcggttc cgcgccgata gatccaacca
     1561 tggcaaggtc atggacaagg ggctctgggg gctgtcgcgc catccgaact acgtcttcga
     1621 atggtttggc tgggtggcct atccggtcat cgccatcgac ctgtccggcg cctggccaca
     1681 aggttgggcg tcgctgatcg cccctgtcgt catgtacctg atcctgacca ggctgaccgg
     1741 cgtgccgcct ctcgagaaaa cgatgatgaa gtcgcggggc aaggtctacg ccgactatca
     1801 gaaccgcgtc ggagccttct tcccccgacg cgctcacaaa cccacgaagt ccgcaggaac
     1861 cgcccgatga gcctcatctc caacagcctc aacaagcttc aggacgcgcc tttcccagat
     1921 ttcatcaccc gcccggcgat cgcactgctg gtggcgaacg cccgcaagtc gctgaaggcc
     1981 ccgccggtcg acatcgaggt gaccttcgcg cgcgagatgg ccgaacggcc ggtcgccgaa
     2041 cacaccgacg ccgcgaacca gcaacattac gaactgcccg cccggttctt cgaactgtgc
     2101 ctcggcaagc accgcaaata ttcaagctgc ttttacgaga gcgaggacgc cacgctcgat
     2161 gaggccgagg ccagcgcgct gcggatcact tgcgagcacg ccgacattca ggacggccaa
     2221 agtgtccttg agctcggctg cggctggggc tcgctgtcgc tttgcatggc cgagcgctat
     2281 ccgaacgcca acatcaccac cgtgtccaac tcacgcagcc agaaggcgtc gatcgacgac
     2341 acggcccgcc gtcgtggcct gacgaacctg accatcatca ccgccgacat gaacgacttc
     2401 acgttggacc agaccttcga ccggatcgtc tcggtggaga tgttcgagca tatggccaac
     2461 tggcgcgccc tgctgggcaa ggcgcgcggt tggctgaagc ccgagggccg gatgttcatt
     2521 cacgtgttca gccacatcga cgccccgtac cggttcgagg tgtcggacca gagcgacttc
     2581 atcgcccagc atttcttcac cggcggcgtg atgccgagcc atggcctgat gaaacagtat
     2641 ggcgacctgc tcaccgtcga ggacgaatgg cgctggagcg ggacgcatta tcagcgcacc
     2701 gccgacgact ggctcgacaa ttttgacaag cacgaagcgg agatccgtgg cctgatggtc
     2761 gaggtatacg gccttgagaa cgccccgctt tggatgcgtc gctggcgccg gttcttcatg
     2821 gccaccgcag ggttgttcgg tgacagcgat ggctcggttt ggggcgtcag ccactaccgc
     2881 ctgaagaggg cttgagcatg atgaaattca tcgccgccta cggctgttcc gcactcgtcg
     2941 tcgcgatcat ggatggtgtc tggctgacca tgatgggcga caagctgtat cgcccggtgc
     3001 tcggcgactt catggccaag tcggtgaaca tcccggccgc gatcgccttc tatttcatct
     3061 atctggccgg tttgaccttc ctcgccactc aaccggcgct gcagcagctt tcggtcggca
     3121 aggcggtggt gaacgcggcg gtcttcggtt tcgtcgccta tgcgacctac gacctcacca
     3181 atcaggcgac gctggcgcgc tggagcacca ccatcacgct cgccgacatg gcctggggca
     3241 cggtcatgag cgttttcgcg gcggttgcgg ggtattacgg atcgcgcctg ctcgtgaggt
     3301 gatcggatct gcaagcagcc tgtcggatgg tcgcggtttg aggcgagtag cgggccatgc
     3361 gaccggcttg gcggcttgaa acggcgaata ggggtttgat ccccttgaca gtccgggcgc
     3421 agcaaagctg gcgctcttag tcgcgtctta agttgcgcga tggcacgatc gattcgtgaa
     3481 gcctagcatc cgcgccatcc gagaaccttg ggccgcctgg aacgacctcc gtcgtccggt
     3541 ctggctgttt gatcctgtcg cccttcgcgg cctctatgcg aacgacgcag cgctcgctct
     3601 gtggggtgct gcgtctctgg aggaactcct cgctcgcgac ttctcaatcc tgtcccctgc
     3661 cgttcgaacc cggacccagc gactggcttg ggcgacagcc ggcggcgagg aggtcagcga
     3721 gcgctggacc ttttatccga acggacaacc ggtcaccgtc caggcggtta tctcgaccta
     3781 ctcactgcca gactccacgt ctgcagcact gctgttcgag gcggcgcctg tagacgccga
     3841 ggctgaggag cgtagagcgc tcgaggcgtt acgtcacacg tccacggcga tcagtctttt
     3901 cgatcaggaa ggccgccccc tgttcgccaa cccggccgct ttcgcggcct atggccggtc
     3961 cgacctgcca ctggcgtcgc gcttcgtcga accagaacgg gctgcccagc tgctatcttt
     4021 ggcgatgtca ggcgagaccg cgaccgatat ctgtcaggta accaccgaga gcggggtccg
     4081 ctggcatcat ctcgacgctc gctccggact ggatccggtc accggctctg caaccctcct
     4141 gctcaacgag cacgacatca ccgataccgt cgaggcgcag accgcccgag ccttggccga
     4201 gcggaagact gcggaatcgg aggcccggca gagctttctc accgaaatgt cgcatgagtt
     4261 gcgcacgcca ttgaacgccg tgatcgggtt tgctgacctg ctctcccgcg cgcaactggc
     4321 cgaggagcat gtcgaacagg cccatagcat tctggagggc gcccgacggc tcgcgaccgt
     4381 ggtcaatgaa atgatccgga ttgcggatgg gacgacggcg ccccccgtat cggtcactgt
     4441 gccgactacc ccgaggtcgc catcggcggc agaggagagg gctacacggg ttttgtatgt
     4501 agatgacaat gcggcgaacc gcagactcgt cgtcaccgtt ctcggcagcc aaggcttcgt
     4561 ttgcgaaacc gctgctgatg gggcggaagg cgtagaggct gcgaagaccg gggactggga
     4621 tgtcat
//
LOCUS       DASVTM010000532         7920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_690576,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000532 DASVTM010000000
VERSION     DASVTM010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690576"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VF633_14675"
     CDS             complement(<1..557)
                     /locus_tag="VF633_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8472353.1"
                     /translation="MTDETRPRGPWDVDPAKEALAVGGPTSREYRLGRIGVRLEKPAR
                     GAVTEDAVHKGQHPAWALISTALLGGFIWFVSGSWVVAVAAIFGLFVHEYGHVLAMNR
                     LGMGPARIYIVPFLGGMARSQRLPESEWHGVLVSLAGPLFGILATLPFFAGWALTGQQ
                     AWLVGAFAISMINLVNLAPAPPLDGS"
     gene            complement(557..1636)
                     /gene="nadA"
                     /locus_tag="VF633_14680"
     CDS             complement(557..1636)
                     /gene="nadA"
                     /locus_tag="VF633_14680"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX8472354.1"
                     /translation="MADGMFGLSERTARETAPVWEKVKDHVTPIEWPAQAELIVEINR
                     LKKERDAVILAHNYMTPEIFHGVGDYVGDSLGLAKEAARSNAKVIVQAGVHFMAETSK
                     ILSPDKTILIPDLKAGCSLAEAITGADVRLIKQRYPGLPVVTYVNTTADVKAETDICC
                     TSANAVQVVEWAAKEWGVDRVILIPDEFLARNVAAQTNIGIIAWKGRCIVHERFTGAD
                     IAEMRAAYPGAEILAHPECPEDVLAAADFAGSTAAMTDYVMAKKPKQVVLITECSMAS
                     NIKGDVPEVNFIGPCNMCPYMKRITLENIRDCLLNMEFEVTVPEEMIARGALAVQRMI
                     DLPPPAIPARYDMIKARHHVAVELI"
     gene            complement(1741..1974)
                     /locus_tag="VF633_14685"
     CDS             complement(1741..1974)
                     /locus_tag="VF633_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8472355.1"
                     /translation="MAEPRLEVRLNAARTEAGLTQADLAERAGVSRKTVNTVENGVFT
                     PSVLVALSLARALDTTVEALFTLAPEQQIWRGG"
     gene            complement(1967..2356)
                     /locus_tag="VF633_14690"
     CDS             complement(1967..2356)
                     /locus_tag="VF633_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472356.1"
                     /translation="MPATSLYLTFKGAQGAWSILGGRGEGYDWAFAFLGPLMSVTVLV
                     MVAGLDNPGDRRMKRLLDDELVRSFRHRSLTVGFTVLLVGFLGAFAAALWRPAVGITA
                     MPLVLAVAAGAAAIRFAWLDHKADPHG"
     gene            2818..4332
                     /gene="ilvA"
                     /locus_tag="VF633_14695"
     CDS             2818..4332
                     /gene="ilvA"
                     /locus_tag="VF633_14695"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX8472357.1"
                     /translation="MQDTIRRILTARVYDVAEETSLDALARLSHRLGRPTWLKREDLQ
                     PVFSFKIRGAYNRIANMTEAELARGVICASAGNHAQGVAMAAATRNAKATIVMPVTTP
                     GIKVAAVRARGGDVILHGDGFDDAYAHAREIEARTGATFVHPFDDPDVIAGQGTIGLE
                     ILRQHPDPIEAIFVPIGGGGLAAGVATIVRFLRPETKIIGVEPVDAPTMKASIDAGHV
                     VTLDQVGLFADGVAVRRAGDHTFRLCNELLDDIVLVDTDAICAAVKDIFDDVRAVAEP
                     AGALALAGMKAWSAANPGSGALVAINSGANLNFDRLRHVAERAEIGEGGEALLAVALP
                     DDRDAYKRFLRSLDGRSVTEFNYRWSATGAAHFFVGVAIKHGPAEKEELISGLHADGY
                     SVEDMSDNETAKLHVRYMVGGRASGLGDERILRFQFPERPGAFLRFLNSLQPAWALTL
                     FHYRNHGDDVGRVLAGISVQPGEREALTAALIDLGYPYIDETDNPASRLFLDGV"
     gene            complement(4329..5159)
                     /locus_tag="VF633_14700"
     CDS             complement(4329..5159)
                     /locus_tag="VF633_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472358.1"
                     /translation="MAVMVVGVICAPSLAAAQDDDDWEFGEDAARQLTVATVRYDGGK
                     AVVVQCEKAQLKVVIVGLPATITVTRYLNVTRADGRSDRQGWFAASGQTVFTSTVAGR
                     DARFLRGGGVFDLRSAASDAAPVHATFDLPPQNANLDRVLTACGYAATDERDLLQRAD
                     EDLKTEFQAEHEHDKPGVQQRSRSISGARPRPAAPSTPPLPRPADVSCIVRNAVYEDC
                     RMEHSIAATSRDGAAWLGRLNGTKLEPVSAAANEGRVVYPGSVSGLVTVVDYIATIPG
                     "
     gene            complement(5489..5644)
                     /locus_tag="VF633_14705"
     CDS             complement(5489..5644)
                     /locus_tag="VF633_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472359.1"
                     /translation="MASRQTPADDGSIARDIAAVSAIDAVSQILDVVCRITGMGFAAV
                     ARVTDTH"
     gene            complement(5619..6188)
                     /locus_tag="VF633_14710"
     CDS             complement(5619..6188)
                     /locus_tag="VF633_14710"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8472360.1"
                     /translation="MLTLDRIETPVGTVLLVVDAEGAVRALDFAGYEDRMMRLLRRHY
                     GDAPLTDGRAPEPVREAVAAYFAGDIAALDGLTVRTGGTEFQRAVWAALRDIPAGQTR
                     TYGQLATAIGRPKAVRAAGLANGQNPVAVIVPCHRVIGANGTLTGFAGGLERKQWLLD
                     HEARPAKKRPFKVSLHSASVGDGFSANAG"
     gene            complement(6196..7332)
                     /locus_tag="VF633_14715"
     CDS             complement(6196..7332)
                     /locus_tag="VF633_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX8472361.1"
                     /translation="METDRTALDQEACYRAVLTRDVRFDGRFFGGVTSTGIYCRPVCP
                     ARTPRRENMIFYPTAAAAEEAGFRACLRCRPETAPDMGAWRGTSNTVSRALSLIEAGA
                     LDEADVETLAGRLGVGERQLRRLFRQHLGAAPVSVAQTRRVLLAKALIHETDLTMAEV
                     ALASGFGSVRRFNETFQSLYGRPPSALRRRKERVSDGGVRLSLPYRPPYDWDAMLAAL
                     AARGDAVEGRMWSRTLDMSLDGAEGRVCVTCDGAGRVEDRVQVRVETSDLRVLPGVLA
                     RVRRVFDLSADPDAIARDLSVDAALHDSLRARPGLRLPGDWVDSGVEPPNDRLPEDFD
                     ARLIGRTEMWRPWRAYGALHLLTAEGAKLMEVYDGRQDDDARAA"
     gene            complement(7396..>7920)
                     /locus_tag="VF633_14720"
     CDS             complement(7396..>7920)
                     /locus_tag="VF633_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472362.1"
                     /translation="ALGSAAAWTVLLLWWIGFLAISPMRPEDLAGRAMADGVDYGAIL
                     SQVLAILYWPGVIYAGCRILFDLIRAAHPAHVRLTASGDLAFGAAQLGLYFWLWTASP
                     LSPVIHVDTLQAFVDRVRAMFESGDWTLSTILMAGVVFGIVQTLFKMSGALWRLITGR
                     LMPYDKRAQGAVAA"
BASE COUNT         1438 a         2826 c         2346 g         1310 t
ORIGIN      
        1 gacccgtcca acggtggcgc aggcgcgaga ttgaccagat tgatcatgga aattgcaaag
       61 gccccgacga gccatgcctg ttgcccggtc agagcccagc ctgcgaagaa cggcagggtc
      121 gccagaatcc cgaacaacgg ccccgccaaa gagaccagca cgccgtgcca ttcgctttcc
      181 ggcagcctct ggctccgcgc catcccgcca aggaacggca cgatatagat ccttgccggc
      241 cccatcccga gccggttcat cgccagaacg tggccgtact catgcacgaa caggccgaag
      301 atcgcagcga ccgccacgac ccagctcccg ctgacaaacc agatgaagcc acccagcagc
      361 gcggtcgaga tcaacgccca tgccggatgc tgacccttgt ggaccgcgtc ctcggtcacc
      421 gcacctcgag ccggcttttc cagccgcacc ccgatccgtc ccagcctgta ttcgcggctg
      481 gtgggaccgc cgaccgctag ggcttccttc gccggatcga catcccaagg gccacgcggc
      541 cgggtttcat ccgtcatcag atcagctcca ccgcgacgtg gtgtcgggcc ttgatcatgt
      601 catatcgtgc cggaatggcg ggcggcggca ggtcgatcat ccgctgcacg gccaaggccc
      661 ctcgcgcgat catttcctcg ggcaccgtga cctcgaactc catgttcaac aggcagtcgc
      721 ggatgttctc cagcgtgatc cgcttcatat aggggcacat gttgcacggg ccgatgaagt
      781 tcacctcggg cacgtcgccc ttgatgttcg aggccatcga gcattcggtg atcagcacaa
      841 cctgtttggg ctttttggcc atgacgtagt cggtcatcgc cgccgtcgat ccagcgaagt
      901 cggcggccgc gagcacgtcc tcggggcatt cggggtgggc gaggatctcc gcgcccggat
      961 aggcggcgcg catctcggcg atgtcggccc cggtgaagcg ctcatgcacg atgcagcggc
     1021 ctttccaggc gatgatgccg atgttggtct gggccgcgac gttgcgcgcc aggaactcgt
     1081 cggggatcag gatgacccgg tcgacgcccc attccttcgc cgcccattcc accacctgaa
     1141 cggcgttggc gctggtgcag cagatgtcgg tctcggcctt cacgtcggcg gtcgtgttca
     1201 cataggtgac caccggcaga cccggatagc gctgcttgat cagccgcacg tcggcgcccg
     1261 taatggcctc ggccagcgaa cagccggcct tcaggtcggg gatcaggatg gtcttgtccg
     1321 ggctcaggat cttggacgtc tcggccatga agtgcacgcc cgcctggacg atgaccttgg
     1381 cattcgaccg cgcggcttcc ttggccagcc cgaggctgtc gccgacatag tcgcccacgc
     1441 cgtggaagat ctccggcgtc atatagttgt gggccagaat gacggcgtcg cgttccttct
     1501 tcagccggtt gatctcgacg atcagctccg cctgcgccgg ccattcgatg ggcgtgacat
     1561 ggtccttgac cttctcccac accggagccg tttcgcgcgc cgtgcgttcg gataagccga
     1621 acattccgtc agccatctga cattcctccg gcgaactcgg ccgcctctcg acttatgctc
     1681 aatattagca taagtgggtg taatgtagtc gcgccggtgg tgaacgcaag ggcagatcta
     1741 ctagcctcct cgccagatct gctgctccgg agccaaagtg aacagggctt cgaccgtggt
     1801 gtccagcgcc cgcgccagag atagcgcgac cagcacggag ggagtgaaga cgccgttttc
     1861 cacggtgttg acggttttgc gcgacacgcc tgccctctca gccaagtccg cctgggtcag
     1921 tcccgcctcg gttcgcgccg cgttaagccg cacctccagt cgcggctcag ccatgggggt
     1981 cggctttgtg gtccagccaa gcgaaccgga tcgcggcggc tccagcggcc actgccagca
     2041 ccaggggcat ggccgtgatc cccacggctg gccgccaaag ggcggcggcg aacgccccca
     2101 ggaagccgac gagcaagacc gtgaaaccca ccgttagaga cctgtgcctg aacgacctga
     2161 cgagttcgtc gtcgagaagc ctcttcatgc gccggtcgcc cggattatcg agacccgcga
     2221 ccattaccag cacggtgacc gacattagcg gccccagaaa cgcaaacgcc cagtcatagc
     2281 cctcaccccg accgcccaaa atgctccacg cgccttgagc gcccttgaag gtcagataca
     2341 ggctcgttgc cggcataatg agtatctgtt cggcccgctg actctgaagc cgatcacgcg
     2401 gactgcccgc cgctctcaat ctttcggttt cgtcattcgg cctgaaaaac atcatagcga
     2461 cgagaccacc gagcacggcg aaaaccccca gcccgatcag aaaagacaga aagggacgat
     2521 agggctcccc tccgtcggcg tcccccagag agaacccgat ccctgcgagc accatcccca
     2581 aggcgaaggc cgagaccgcg atgaaatggc gtcgccaacg cttgttttcc gcgccttgca
     2641 gtcgctcaag ttccgtatcg tccacccaat cctcccaaag tcaccctcgg gttacatatc
     2701 acctatgggt tacacgcgtc aagactccgc agcctgcaca ttaaatgctc gaccgcgtgt
     2761 gtttagcggc ataagcgaca actccctttc tccctacgcc acttttccga gacgttcgtg
     2821 caagacacca tccgccgcat cctgaccgct cgcgtctatg acgtggccga ggaaacgtcg
     2881 ctggatgccc tcgcccgcct gtcgcaccgc cttgggcgcc ccacatggct caagcgcgaa
     2941 gacttgcagc cggtgttctc gttcaagatc cggggcgcct acaaccgcat cgccaacatg
     3001 accgaggccg aactggcgcg cggggtcatc tgcgcctccg ccggcaatca tgctcagggc
     3061 gtggccatgg ccgccgcgac gcgcaacgcc aaggcgacca tcgtcatgcc ggtgaccacc
     3121 ccgggcatca aggtcgccgc cgtccgcgcg cgcggcggcg acgtcatcct gcacggcgac
     3181 ggcttcgacg acgcctatgc ccatgcgcga gagatcgagg cccgcaccgg cgcgaccttc
     3241 gtccacccgt tcgacgaccc cgacgtcatc gccggtcagg gcaccatcgg gctggaaatc
     3301 ctgcgccagc acccggaccc catcgaggcc atcttcgtgc ccatcggcgg cggcggtctg
     3361 gccgccgggg tcgcgaccat cgtgcgtttc ctgcgccctg aaacgaagat catcggcgtc
     3421 gaacctgtcg acgccccgac gatgaaagca tccatcgacg caggccatgt cgtgacgctg
     3481 gatcaggtcg gtctgttcgc cgacggggtc gcggtgcgcc gtgcgggcga ccacaccttc
     3541 cgcctgtgca acgaactgct cgacgacatc gtgctcgtgg ataccgacgc catctgcgcg
     3601 gcggtgaagg acatcttcga cgacgttcgc gccgtggccg aacccgccgg agcgctggcg
     3661 cttgcaggca tgaaggcctg gtccgccgcc aatcccgggt ccggagcgct ggtcgccatc
     3721 aactcgggcg ccaatctgaa cttcgaccgc ctgcgccacg tcgccgaacg ggccgagatc
     3781 ggcgagggcg gcgaggcctt actggccgtc gccctgcccg acgaccgcga cgcctacaaa
     3841 cgcttcctgc gctcgctcga cggccgctcg gtcaccgagt tcaactatcg ctggtcagcc
     3901 accggcgcgg ctcatttctt cgtcggcgtg gccatcaagc atggtccggc cgagaaggaa
     3961 gagctgatct ccggcctgca cgccgacggc tattcggtcg aggatatgag cgacaacgaa
     4021 accgccaagc tgcacgtccg ctacatggtc ggtggccgcg cctcgggtct cggcgacgag
     4081 cggatcctgc gcttccagtt ccccgaacgc cccggcgcct tcctgcgctt cctcaacagc
     4141 ctgcaacccg cctgggccct gaccctgttc cactaccgca accacggcga cgacgtcggt
     4201 cgggttctgg caggcatctc ggtccagccg ggcgagcgcg aagccctgac cgccgccctc
     4261 atcgacctcg gctatccgta catcgacgag accgacaatc ccgccagccg cctgttcctc
     4321 gacggcgttt aacccggaat agtcgcgata tagtcgacaa cggttacgag gccactgacc
     4381 gaccccggat agactactcg accttcattg gccgcagcgc tgacgggttc gagcttcgtg
     4441 ccgttcaaac gtcctaacca agcggctcca tctcgagagg tagccgctat ggaatgctcc
     4501 atccgacagt cttcatagac ggcattacgc acaatacagc tcacatccgc cggcctggga
     4561 agcggcggcg tcgaaggcgc cgctggccgg ggtcgggccc cgctgatgct ccggcttctc
     4621 tgctgcaccc caggtttatc atgctcatgc tcggcctgaa attcggtttt caggtcttca
     4681 tcggcccgct gaagcaagtc gcgctcgtcc gtcgccgcat agccacaggc cgtcagcacc
     4741 cgatcgagat tggcgttctg aggcggcaga tcgaatgtcg cgtgcacagg cgccgcgtcg
     4801 ctagcggctg accgcagatc gaacacgccg ccgccacgca ggaaccgcgc gtcccgtccc
     4861 gctaccgtgc tggtgaaaac cgtctggccg gaagccgcaa accaaccttg acgatcactt
     4921 cgaccgtcgg ctcgggtcac attgagatag cgcgtaacgg ttatcgtcgc cggcaaaccc
     4981 acgatcacga ccttaagctg agccttctcg cactggacga cgacagcctt gccgccgtca
     5041 taccgcacgg tcgcaacggt cagttggcgc gcggcatcct cgccaaactc ccagtcgtcg
     5101 tcatcctgag ccgccgccaa tgacggcgcg cagatgaccc cgaccaccat gaccgccaac
     5161 agcgcacgca gaccagaaaa ccgcccattg caacgctccc caaacagaat tcggccgatc
     5221 agacgataag ctcaaaccgc atcctctggc gagggggagc agtcgcagtg tctaaccgcg
     5281 aagtgaatcc tcgatcgtcg ccctgaccgg catcacaaac cgtaagttct gggcgtgtgg
     5341 tgatcgcgat aggcggggtc agtttgcgca tggtcgaata cgaccgattg tcggtggccc
     5401 cggatgtcat cgcagagcgt ggtttcaagc ggcaactcgc ctccgaccgg aagaccgaaa
     5461 ccgatgttgt ccagcaccgc gcaggccgtc agtgagtgtc ggtcacccgc gcgacagcgg
     5521 cgaaccccat gccggtaatg cggcagacga cgtcgagaat ctggctgacc gcgtcgattg
     5581 ccgacaccgc cgcgatgtcg cgcgcgatgg agccgtcgtc agccggcgtt tgccgagaag
     5641 ccatccccga cgcttgcgct atgaagcgac accttaaatg gtcgtttctt tgccggtcgt
     5701 gcttcgtgat ccagcaacca ctgcttgcgt tccagaccgc ctgcgaaccc ggtgagcgtg
     5761 ccgttcgctc cgatcacccg atgacatggc acgatcaccg cgacggggtt ctgcccgtta
     5821 gccagcccag ccgcccgcac cgctttcggc ctgccgatcg ccgtcgccaa ttgtccatag
     5881 gtgcgggtct gtcctgcggg gatatcgcgt agcgccgccc acaccgcccg ctgaaattca
     5941 gtcccgcctg tcctgaccgt caggccgtca agcgccgcga tatcgcctgc gaaataggcg
     6001 gcgacagcct cgcgcacggg ctccggcgca cgaccgtccg tcaacggagc atcaccgtag
     6061 tgcctgcgca gcagccgcat catccggtct tcataaccgg cgaagtcgag cgcccgcacc
     6121 gcgccttcgg catcgaccac cagcagcacc gttccgaccg gagtctcgat ccggtctaac
     6181 gtcagcatca cggtttcagg ccgcgcgcgc gtcatcgtct tgcctgccat cataaacctc
     6241 catcagcttc gcgccttctg ctgtcagcag gtgcagcgcg ccataggccc gccagggccg
     6301 ccacatctca gtccggccga tcagccgagc gtcgaaatcc tcgggtaacc gatcatttgg
     6361 cggctcgacc ccgctatcga cccagtcgcc cggcagccgc agtccgggtc gcgccctgag
     6421 actgtcatga agcgcggcat ccaccgagag atcgcgggcg atggcgtccg gatctgctga
     6481 cagatcgaac acccgcctca cgcgcgccaa caccccgggc aggacacgca gatcggacgt
     6541 ctccacccgg acctgaacac ggtcttcgac ccgccccgcg ccatcgcacg tcacgcagac
     6601 ccgaccttcg gcgccgtcca gcgacatatc cagcgtcctc gaccacatcc ggccttcgac
     6661 cgcatccccg cgcgccgcca gcgccgccag cattgcgtcc cagtcatacg gcggccgata
     6721 cggcaggctc agtcgcacac cgccgtcgct gacacgctcc ttgcggcggc gcagcgccga
     6781 gggcgggcgt ccatagaggg actgaaaggt ctcgttgaag cgccgcacgc tgccgaaccc
     6841 cgaggccagc gccacctcgg ccatggtcag gtcggtctcg tggatcagcg ccttggccag
     6901 cagcacgcgc cgggtctggg ccacgctgac cggcgccgcg cccagatgct ggcggaacag
     6961 tcgccgcagt tgccgctcgc cgacgcccag gcgcccggcc agcgtctcca catcggcttc
     7021 atccagcgcc ccggcctcga tcaggctcag agcccggctg acggtgttcg aggtccctcg
     7081 ccaggcgccc atgtccgggg ccgtctcggg ccgacagcgc aggcaggcgc gaaagcccgc
     7141 ctcctcggcc gccgccgccg taggatagaa gatcatgttc tcgcgcctgg gcgtccgcgc
     7201 cgggcagacc ggacggcagt agatgccggt ggaggtcaca ccgccgaaga accgtccatc
     7261 gaagcgcacg tcccgcgtca gcaccgcgcg gtaacaggcc tcctgatcca gcgcggtgcg
     7321 atccgtttcc atgcggccaa catgagggct gacgcccccc atgtctcgcg gttttcggac
     7381 atcggcgtgg aagtgtcagg cggccacggc tccctgtgcg cgcttgtcgt agggcatcag
     7441 ccgcccggtg atcagccgcc agagggcgcc cgacatcttg aacagcgtct ggacgatgcc
     7501 gaagacgacg ccggccatca ggatggtcga gagcgtccaa tcgccgcttt cgaacatggc
     7561 ccgaacccga tcgacgaacg cctgcagcgt atcgacatgg atcaccggcg acagcggcga
     7621 tgcggtccat agccagaagt acaacccgag ctgtgcagcg ccgaacgcca gatcccccga
     7681 cgccgtcagc cgcacgtgag cagggtgggc cgcccggatc aggtcgaaca ggatccggca
     7741 accggcatag atcactcccg gccagtaaag gatcgccaga acctggctca ggatcgcgcc
     7801 ataatcgacg ccgtccgcca tcgcgcggcc ggccagatcc tcgggcctca tcggcgagat
     7861 ggccagaaat ccgatccacc acagcagcag caccgtccac gctgccgcgc tgcccaaggc
//
LOCUS       DASVTM010000533         5406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_583438,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000533 DASVTM010000000
VERSION     DASVTM010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583438"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="VF633_14725"
     CDS             complement(<1..1624)
                     /locus_tag="VF633_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8472363.1"
                     /translation="MTSINNGYLLGLYGGSYDPSAGSALTAAITKKAQPTAPWATGVK
                     ATDPSALIRAALAGRNIINENAAQLDVKGASADYKKLFAMYQGLETLSALTNRSSTSG
                     LTATETTQLQKRFAEGLAELTAYVTKADFDGVRVVQGTSTSISKTTAAVPRDSAVSIT
                     GPVHEGSLDTPVTAFQGDTKFNITIKVPVGLSTNTTSVPIDLSGMGSTPRTMDNVLGY
                     INKQLETAGFQTRIGRQQIKEEPRTVQVNGKAVTLPAGPDKWALAIRGASTETVGFNA
                     AATSDAVYVLQTAGNAAQTTKTTVNGVTTTKTTAASTLTNQILKFQADGGAAAAPTAG
                     NIGETQWVDGRLSQETLPAGVQTVRASAVGPDGSLWIVADVSSGPDTQPIKGVQDVAL
                     MKYDSAGRLVATRTLGAASTASGYALAIDPNGNVAVAGSVTGALATSTTDAGKTGEVA
                     GVADSFVTVFDKDGTEQWTQRRGARAADEATTVSFGANGAVYVGGRSKSAMPGSTTTG
                     GWDGYVQTFKAGEAYPTAGIVAKALGGTQFGTS"
     gene            complement(1924..2754)
                     /locus_tag="VF633_14730"
     CDS             complement(1924..2754)
                     /locus_tag="VF633_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX8472364.1"
                     /translation="MSNSVLTNSAAAIALQNLSATNSKLTTTQGRISTGLKVQGAKDN
                     AAIWAIAQKQRADVGSLSAVKSSLDRATSIADVALSAGETVSDLLNQLREKVVAAKDS
                     SLKTQSRQLLDGDFKALLKAISSAVENASFDGGNILNGSQPTGLKFLANADASAFVTL
                     SGKNLSLSGSILSLGLSDSLLTFTSAGTALTKLDSSITAVNAALGAIGAQAKQIQAHN
                     TFVSKLSDTLESGIGNLVDADLAKESARLQALQVQQQLGAQSLSIANQSPQVILSLFQ
                     "
     gene            complement(2907..3170)
                     /locus_tag="VF633_14735"
     CDS             complement(2907..3170)
                     /locus_tag="VF633_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HEX8472365.1"
                     /translation="MSIVAPVPPVAPAITGDEQSAPRASSDAEPAPQYRLVIEEGPTS
                     GSFIYKTLDRATGEVVRQFPREQVLRLAEARRYDAGSVIDTNA"
     gene            complement(3279..4112)
                     /locus_tag="VF633_14740"
     CDS             complement(3279..4112)
                     /locus_tag="VF633_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX8472366.1"
                     /translation="MALNSVNTNSGALVALQNLNATNKELDTVQSRINTGKKVNSAKD
                     NGAIWAIAQGQRSEISALGAVKDSLARGSSAVDVSLAAGESVSDLLTQLKEKALAATD
                     KSLTTSARKALNEDFKAVRDQITTVVSNAKFNGVNLLDNSTGTAGYKALSNTTGSTLK
                     VAGENLSLGGANVDVTAAMTIGTSTLAATALTAVNTSIDKVSASLARLGTGSKSLETH
                     ATFVGKLSDALESGVGNLVDADLAKESARLQSLQTKQQLGVQALSIANQSTSVLLGLF
                     R"
     gene            4411..4800
                     /locus_tag="VF633_14745"
     CDS             4411..4800
                     /locus_tag="VF633_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472367.1"
                     /translation="MAAASGIEASIQLEMRTVPQGSPVAAPDQRRPQLDSSCLYRLGD
                     ARIQGLVPADLALYQGGEIDCMAGDVARFQGAAGARLDQSSLSGRGSQALGNEKSTGL
                     RSIAASITATSAGWTPFIRSRERRERE"
     gene            complement(4802..5107)
                     /locus_tag="VF633_14750"
     CDS             complement(4802..5107)
                     /locus_tag="VF633_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472368.1"
                     /translation="MTEPIAPLIPTPTPSPLAEGRWLWRRIYVFSISLGLWKLLADVV
                     RTATPEALPHISDGLIALLALLLLLYLVAPTAQQLVALMAEMKLRLGGIASGLKVDR"
BASE COUNT          926 a         1651 c         1774 g         1055 t
ORIGIN      
        1 tcgaagtgcc gaactgggtc ccgccgaggg ctttggctac gatcccggct gttggataag
       61 cctcgcccgc cttgaacgtc tgaacatagc cgtcccaacc gccggtcgtc gtgctgccgg
      121 gcatggccga tttcgatcgg ccgccgacgt agacggcgcc gtttgctcca aagcttaccg
      181 tcgtagcctc atccgccgca cgagcgccgc gccgctgggt ccattgctcc gtaccgtcct
      241 tgtcgaagac ggtgacgaaa ctgtcggcga cgccggccac ctcgccggtc ttgcccgcat
      301 cagtagtgga cgtcgccaac gcgcccgtga ctgagcccgc gaccgcgacg ttgccgttcg
      361 ggtcgatggc gagggcgtat ccgctagccg tcgaggcggc tccgagcgtg cgtgtcgcga
      421 ccagccgacc agcgctgtcg tacttcatca gggcgacgtc ctgaacgccc ttgatcggct
      481 gcgtgtcggg accggagctg acgtcggcaa cgatccagag tgacccatca ggaccgaccg
      541 cgctagcccg taccgtctgg actccagcgg gcaaggtttc ctgcgatagc cgtccatcga
      601 cccattgggt ctcgccgata ttgcctgccg tcggcgcagc cgcagcgccg ccgtcagcct
      661 ggaatttcaa gatctggttg gtcagggtgg acgccgccgt cgtcttggtc gtcgtcaccc
      721 cgttgaccgt cgtcttcgta gtttgggcgg cgttaccggc agtctgtaag acatagacgg
      781 catccgaagt cgcggccgcg ttgaagccga cggtttcggt ggaggctccg cggatggcca
      841 aggcccattt gtcagggccc gcaggcaagg tcacggcctt gccgttgacc tggacggtcc
      901 tgggctcttc cttgatctgc tggcggccga tgcgggtctg gaacccggca gtttcaagct
      961 gtttgttgat atagcccaag acgttatcca tcgtcctggg cgttgatccc atgcccgaca
     1021 gatcgatcgg aaccgaggtg gtgttcgtcg acaagccgac cgggaccttg atggtgatgt
     1081 tgaacttcgt gtcgccctga aaggcggtaa ccggcgtgtc cagcgaacct tcgtgcacgg
     1141 ggccggtgat cgagacggcg ctgtcgcggg gaacggcggc cgtcgtcttg ctgatcgagg
     1201 tcgatgtgcc ctgcactacc cgcacgccgt caaagtcggc cttggttaca taggcggtga
     1261 gttcggcaag accttcggcg aaccgtttct gcagctgggt cgtctcggtc gcggtgagcc
     1321 cgctcgtcga agaccggttg gtcaaggcgc tcagggtctc cagaccctgg tacattgcga
     1381 acagtttttt gtagtcggcc gaggcccctt tgacgtcgag ttgcgccgcg ttttcgttga
     1441 tgatgttgcg gccggcgagc gccgcgcgga tcagggccga cggatcggtg gccttgacgc
     1501 cggtggccca cggcgcggtc ggttgggctt ttttggtgat cgcggccgtc agggcgctgc
     1561 cggccgaggg atcataactc ccgccgtaca aacccagaag atagccgttg ttgatcgacg
     1621 tcactgaagc ctccgtgcca cagtatggct ggtgagaggc aacggaccat taacagaatc
     1681 cggaaccact ccgaaaatcg cacgttgatc ggccgcagga gagttgggtc agacgagcgc
     1741 aagcgacatc ggcccccagg ctctcaatct tctggagcgt cgcgatgagc tttccgtccg
     1801 tggttcagtc ccttctcgcc ctttacgggt tcggcgaccg cgcccatgcg acacgccgcc
     1861 gtcccggtcc ggtgtcgata ggcggctcac agccgaggcg ctgagcccgc gttcagcccg
     1921 gtcctactgg aacagcgaca gtatgacctg tggcgactgg ttggcgatcg atagcgactg
     1981 ggcgcccagc tgctgctgaa cctgcagagc ctggaggcga gcgctttcct tggctaaatc
     2041 cgcgtcgacg aggttgccga tacccgactc cagcgtatcg ctgagcttgc tcacaaacgt
     2101 gttgtgcgcc tggatttgct tggcttgagc cccgatcgcg ccgagagcgg cgttcacggc
     2161 ggtgatcgag ctgtcgagct tggtcagggc ggtgccggcg gatgtgaacg tcagcaggct
     2221 gtcgctgagc ccaaggctca ggatcgatcc ggacaggctg aggtttttgc ccgacagggt
     2281 aacaaaggcg ctggcatcag cgttggcgag gaatttcagg ccggtcggct gagacccgtt
     2341 aagaatgttt ccgccgtcaa agctggcgtt ctcgaccgcc gagctgatgg cctttagcag
     2401 agccttgaag tcgccatcca gcagctgacg cgactgggtc ttaagcgacg aatccttggc
     2461 cgccacgacc ttttctcgaa gctggttcaa caggtccgac acggtttcgc cagccgaaag
     2521 cgcgacgtcg gcgatggatg tcgcgcgatc gaggctcgac ttgacggctg agagagagcc
     2581 gacatccgca cgctgcttct gggcgatggc ccatatggct gcgttgtctt tggcgccctg
     2641 caccttcaga ccggtgctga tacggccctg cgtagtcgtc agtttggagt tcgtagcgct
     2701 gaggttctgg agcgcgatcg cggctgcaga attggttagg acgctgttgc tcatcgtttt
     2761 gaaagccccg gaaatgttcc gacaggctat ggttaacaag tgagcgtatg gttaacggcc
     2821 gataaccacg ccgattaacc acgcgggagg atccctgcgc ggcggttgtg gtttcttggc
     2881 ctctcccgga aagaaccgac caggactcag gcgttggtgt cgatcaccga tcccgcgtcg
     2941 tatctccggg cttccgccag acgcagcacc tgttcgcgag ggaactggcg gacgacctct
     3001 ccggtggcgc ggtctagggt cttgtagatg aacgacccgg aggtagggcc ttcctcgatc
     3061 accagccgat actgtggggc tggttcagcg tcgctggacg ccctgggggc ggactgctcg
     3121 tctccggtga tcgcaggtgc gacgggcgga acgggcgcga cgatactcaa cttgacatgt
     3181 gtttccatga ctccccaagc cgaaacccag attcggcttc cctaaaaagg acgatcggaa
     3241 aggtcgggcg ggaccgaagc cccgcccttc cgagtgtctt agcggaacag gcccaacagg
     3301 accgacgtcg actggttggc gatcgacaga gcctgcacac cgagctgttg cttggtttgc
     3361 agcgactgca gtcgggcgct ttccttggcg aggtcggcgt cgacaaggtt gcccacgccc
     3421 gattccagcg cgtccgacag cttgccgacg aacgtcgcgt gggtttccag cgacttggag
     3481 ccggtgccca ggcgagccag ggaggccgag accttgtcga tcgaggtgtt cacggcggtc
     3541 agagccgtgg cggcgagcgt ggaggtgccg atggtcatcg ccgccgtaac gtcgacgttg
     3601 gcgccgccga gcgaaaggtt ctcgccggca accttgaggg ttgaacccgt ggtgttggac
     3661 agagccttgt agccagccgt gccggtcgag ttatccagca ggttcacgcc gttgaacttg
     3721 gcgttcgaaa cgacggtcgt gatctgatcg cgaacggcct tgaagtcctc gttcagggcc
     3781 ttgcgggcag acgtagtcag cgacttgtca gtcgcggcca gggctttttc tttcagttgg
     3841 gtcagcaggt cggaaaccga ctcgccggcg gccaggctga cgtccacggc agacgaacca
     3901 cgggccaggc tgtccttgac ggcgcccagt gccgagattt ccgaacgctg gccctgagcg
     3961 atagcccaga tagcgccgtt gtcttttgcg gagttgacct tcttgcccgt gttgatccgg
     4021 ctctggacag tgtccagttc cttgttcgtg gcattgaggt tctgaagggc gacgagggcg
     4081 cccgagttcg tgtttacgct gttcagcgcc atgacgaata gccttgtttc aaggtgtccg
     4141 accgccattt tagccgtcgg gagcattttg ctcgagagac caagagcaag ggctgcgcca
     4201 acacgccggg tgaggtcgag aaggcatgcc aaaccccgcg accattgttt tttctacggt
     4261 actttagctt aaccatgctc cccgcccggc aagccctgcc gggtagcgcc ggttccccct
     4321 gcagaagctg ccgggcgccc ggcagagcct gccgggcgcc cggaaggacg aaccgtctgc
     4381 cccaagagcc taccgccaga tacggcccac gtggccgccg cctcagggat agaagcctcg
     4441 atccagctcg aaatgcggac cgtcccgcaa ggaagcccag tcgccgcccc agaccaaagg
     4501 cgtcctcaac tcgatagcag ctgcctttac cgcctcggcg atgcgcggat acaggggcta
     4561 gtcccagcgg atctggccct ctaccaaggc ggcgagatcg actgcatggc cggtgatgtg
     4621 gcgcgatttc agggtgcggc tggcgcccgc cttgaccaga gcagcctgtc gggccgtgga
     4681 tcgcaggccc tcggtaacga gaaatcgacg gggctgcgct cgatcgcagc ctcgatcacc
     4741 gcaaccagcg ccggatggac gcccttcatc cggtcgcggg aacgccggga aagggagtaa
     4801 ctcatcgatc caccttgagg ccactggcta tgccgccgag acgaagcttc atctcagcca
     4861 tcagcgcgac cagttgctgc gcggttggtg cgacgagata aaggagcagc aagagcgcca
     4921 ggagtgcgat cagcccatcc gaaatgtgcg gcaatgcctc tggcgtcgcc gtgcgcacaa
     4981 cgtcggccag cagtttccac agccccaggc tgatgctgaa gacgtaaatc cgtcgccaaa
     5041 gccagcgccc ttcagccagg ggagatggag tgggtgtcgg gatgaggggg gcaatgggtt
     5101 cagtcattgg tcgtcgtcct gttcaggttc ggtccttggc tggcggcggc catggccgcc
     5161 ggtgtgatcc aggcgatcat cgtgggagag cctgcggggc gcctcccagg tttcgagatc
     5221 aaggagttcg aagtcgatga tttcttcggt ccggggcgag gccagcgtca tcgaggcaag
     5281 cgccgtagat ccggatatca gtggtgcgag ggtcatggtg tcagccaatg gccgtcaggc
     5341 ggacgagacc ggctccggcg gttcctcccg cgccggacgc cgtgacgccc gcgccccctc
     5401 caccgc
//
LOCUS       DASVTM010000534         8675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1061981,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000534 DASVTM010000000
VERSION     DASVTM010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061981"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VF633_14755"
     CDS             <1..679
                     /locus_tag="VF633_14755"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX8472369.1"
                     /translation="ENPNRLQHYYQFQVILKPNPDNLQELYLGSLAAIGIDPKLHDIR
                     FVEDDWENPTVGAWGLGWEVWCDGMEVTQFTYFQGVGGIEVDVVSGELTYGLERLAMY
                     VQGVDNVYDLRFNNHGLSYGDVFLENERQQSEANFHGYDVEGLKRRFEDMEAESTRLL
                     AMTGPQGQHLVLPAYDQVLKASHLFNLMDARGAIAVAERQSYIGRIRDLCKACALAYV
                     EQERKTA"
     gene            679..2751
                     /gene="glyS"
                     /locus_tag="VF633_14760"
     CDS             679..2751
                     /gene="glyS"
                     /locus_tag="VF633_14760"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX8472370.1"
                     /translation="MPQLLLELFSEEIPARMQQGAARDLERMVSERLKTTGLTWDALN
                     VFAGPRRLTLVIDGLPSATPDRSEELKGPRANAPEQALEGFLRKTGLTKDQLTERDGV
                     FFAVISQSGRATADIVPETVDAIVRAFPWPKSMRWGSGTLRWIRPLKRIVCLFDGKVV
                     PFEIDGIQSDAITEGHRFLGKGHPIVVNDFADYRRKLEADYVLLDVADRKLKILEAAN
                     AACAAKGLALVDDDGLLDEVAGLAEWPTPILGDMDPQFLSLPPEVVRLSMKVHQKYFA
                     VRDPAKHGLAPNFVVVANVEASDGGVALAAGNSRVLSARLNDARFFWDEDLKTGFDVW
                     SEKLSGVTFHAKLGTLAERVDRIAALAREIAPLIGADPDEAERAARLAKADLASSMVG
                     EFPELQGVMGGYYARAAGESEAVADAVRDHYKPQGPADSVPTAPLTVAVALADKLDTL
                     VGFFAIDEKPTGSKDPFALRRAALGVIRTLLENEVRLEVREAVSMVHWAHGKLGPHWH
                     RLLDEGSLELEVQDFFADRLKVLLRDQGKRHDLVDAVFALGDDDLVRIVRRVEALDAF
                     LATDDGANLLAGSKRAANILRAEAKKGDVPTGMVQTGLPNQPDAETALAFAVASASTA
                     VDAALDTEDFAAAMTALARLRAPVDAFFTDVMVNSDVPEERANRLKLLGQVHDVMGRV
                     ADFAQIAG"
     gene            complement(2842..3324)
                     /locus_tag="VF633_14765"
     CDS             complement(2842..3324)
                     /locus_tag="VF633_14765"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX8472371.1"
                     /translation="MQRMMRNIFLDRHSRERRSSSSPCAQHGEVAAPASAATEGLLAT
                     QGTVSRARVSRRTLTPPEARLWTRLRRQALAGFKFRRQHPVGCYVLDFYCAQAKLAVE
                     VDGMSHDHPDRMLHDQRRTDWLAREGIAVLRVGAEAVRINLSEVLERIQRETQARVRG
                     "
     gene            complement(3520..4611)
                     /gene="queA"
                     /locus_tag="VF633_14770"
     CDS             complement(3520..4611)
                     /gene="queA"
                     /locus_tag="VF633_14770"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX8472372.1"
                     /translation="MKTADFDFHLPEDHIALRPAEPRDSARLLVVQDGVLDYRVIRDL
                     PAFLRPGDALVFNDTRVIPARLSGVRQRPDPENPGQTLTVAVEATLHHRDAPDRWSAF
                     MKPGKRLKVGDRIRFDRDMDNACMLGQLDAAVAAKSEDGLITLAFNLAGPALDDAIRD
                     VGVMPLPPYIAAKRPEDDRDLTDYQTVFAEHDGSVAAPTAGLHFTPALLEAIRARGVS
                     THAVTLHVGAGTFLPVKADDTADHKMHSEWGEVSPETAAALNAVHENGGRVVCVGTTS
                     LRLLESATTPDGVVQPFHGDTAIFITPGYRFRAVDVLMTNFHLPKSTLFMLVSAFAGL
                     ETMRSAYEHAIADGYRFYSYGDGSLLFRS"
     gene            5315..5725
                     /locus_tag="VF633_14775"
     CDS             5315..5725
                     /locus_tag="VF633_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472373.1"
                     /translation="MTPMAAIAGARLAHRDTPGKLVLLDHPLFRYGALAMGVGELFGD
                     KMKSAPDRTVFLGLLARVLSAGIAGAALAPRGREKVGAALAIGTAVPLAYLGLAGRKR
                     AMAEIGQTKSGLIEDALIVAAGAAVVAWVVGRNR"
     gene            5775..7451
                     /locus_tag="VF633_14780"
     CDS             5775..7451
                     /locus_tag="VF633_14780"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX8472374.1"
                     /translation="MLQNLETLAREAKSWPFEQARNLLAHVLKKRLTDAERTAAKLLI
                     DAGKADEALATFEVLQRPVILETGYGPSGLPHMGTFGEVARTTMVRNAFRALTGDHWP
                     TRLIAFSDDMDGLRKVPEGVPNPEMLREDLHLSLTRVRDPFGTHESFGAHNNARLRAF
                     LDGFGFDYEFMSSTQTYKSGRFDATLLTLLERFDAMMAIMLPTLGEERRATYSPFLPV
                     SPITGHVLQVPTLARNVEKGTITFEDSAGEPGNRTEVPVTGGHVKLQWKPDWAMRWVA
                     LDVDYEMSGKDLIESVRESGKICRALGGVPPEGFNFELFLDIEGKKISKSKGNGLTMD
                     EWLRYGTPESLSWYMFQSPKSAKSLHFNVIPRAIDDYLSFIEAYKTQEPAKQIDNAVW
                     AIHAGSPPSTVSPVSFALLLNLVGVANASTKDQLWAYFAKYLPEATPDNEPVLDRLMG
                     HALNYYEDFVKPSKSYRLPDDKEKAALLDLAGRLKALPADSTDGEVIQSEVYAVGKEH
                     GFEPLRGWFGALYEVLLGASQGPRFGSFAAIYGLPQTIQLIEDGANGQLV"
     gene            7535..7915
                     /locus_tag="VF633_14785"
     CDS             7535..7915
                     /locus_tag="VF633_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472375.1"
                     /translation="MRHTMDQDQRIFHLDLNRSDSARDALEQSLTILGNHPEIWAWDW
                     IVDAQRVPEDASVEQIARLAEVFATVSATRPVVVATTAFVSADRYLHLWARVMDYQFP
                     KRKHLVVTDVAQAVKLIASRRVGR"
     gene            8006..8470
                     /locus_tag="VF633_14790"
     CDS             8006..8470
                     /locus_tag="VF633_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX8472376.1"
                     /translation="MSKTLNTTIATAAALAALLTAAPMAASAQSNGITSCDAPGGRQR
                     AGAVIGGILGGVVGNNLARNERTAGTIVGAGAGAAAGSYIGCNQQRARSASARGEYRA
                     TSNLRIRSGPGTNYGTRGTLQAGQPFSVVSSSGEWVQIAGGGWVNARYVTTN"
BASE COUNT         1570 a         2952 c         2667 g         1486 t
ORIGIN      
        1 cgaaaatccc aaccgcctcc aacactatta ccaatttcag gtgatcctga agccgaaccc
       61 cgacaacctt caggagctct atctcggctc gctggcggcc atcggcatcg acccgaaatt
      121 gcacgacatc cgcttcgttg aggacgactg ggagaacccc acggtcggcg cctggggcct
      181 cggctgggaa gtctggtgcg acggcatgga ggtgacccag ttcacctatt tccagggcgt
      241 cggcggcatc gaggtcgacg tcgtctccgg cgaactgacc tacgggctcg agcgtctggc
      301 catgtacgtt cagggcgtcg acaacgtcta tgacctcagg ttcaacaatc atggcctgag
      361 ctacggcgac gtcttcctcg agaacgaacg ccagcaatcc gaggcgaact tccacggcta
      421 tgacgtcgaa ggcctgaagc gccgcttcga ggacatggag gcggaatcca cgcgcctcct
      481 cgccatgacc ggcccccaag gccaacacct tgtcctgccc gcctacgacc aggtcctcaa
      541 agcctcgcac ctcttcaacc tgatggacgc ccgcggcgcc atcgccgtcg ccgaacgcca
      601 gagttacatc ggccggatac gagacctgtg taaggcctgt gccctcgctt atgtcgaaca
      661 agagcggaaa accgcctgat gccccaactt ctcctcgaac tcttctccga agaaatccct
      721 gcgcggatgc agcagggcgc cgcgcgtgat cttgagcgca tggtctccga acggctgaag
      781 actaccggcc tgacgtggga tgcgctgaac gtgttcgctg gaccgcgtcg tttgacgctg
      841 gtgatcgacg gcctgccgtc cgccacgccc gatcgctcgg aagagctcaa gggtccgcgc
      901 gccaacgccc ccgaacaggc gctggaaggc ttcctgcgca agaccggcct gacgaaggac
      961 caactcactg aacgcgacgg cgtcttcttc gccgtcatca gccagtcggg ccgcgccacc
     1021 gccgacatcg tccccgagac cgtcgacgcc atcgtccgcg ccttcccctg gcccaagtcg
     1081 atgcgctggg gctctggcac gctgcgctgg atccggccgc tgaagcggat cgtctgcctg
     1141 ttcgacggca aggtcgtccc cttcgagatc gacggcatcc agtccgacgc gatcaccgag
     1201 ggccaccgct tcctcggcaa gggccacccc atcgtcgtca acgacttcgc tgactatcgc
     1261 cgcaagttgg aggccgacta cgttctgctc gacgtcgccg accgcaagct gaagatcctc
     1321 gaagccgcca acgccgcctg cgccgccaag ggcctcgcgc tggtcgatga cgacggtctg
     1381 ctggacgagg tcgccggtct cgccgaatgg ccgacgccga tcctcggcga catggacccc
     1441 cagttcctca gcctgccgcc cgaggtggtc cggctgtcga tgaaggtcca ccagaagtat
     1501 ttcgccgtcc gagatccggc gaaacacggt ctcgccccca acttcgtcgt cgtcgccaat
     1561 gtcgaggcct ccgacggtgg cgtcgccctg gccgccggca acagccgcgt cctgtccgcc
     1621 cgcctgaacg acgcccgctt cttctgggat gaggacctca agaccggctt cgacgtctgg
     1681 tccgaaaaac tgtccggcgt caccttccac gccaagctcg gcacattggc cgaacgcgtc
     1741 gaccgcatcg ccgcccttgc ccgcgagatc gccccgctga tcggcgccga tcccgacgaa
     1801 gccgaacgcg ccgcccgcct cgccaaggcc gacctcgcca gctccatggt cggcgaattc
     1861 cccgaactgc aaggggtgat gggcgggtat tacgcccgcg ccgccggtga atccgaggct
     1921 gtcgccgacg ccgtccgcga ccactacaag ccgcagggcc ctgcagactc cgtcccgacc
     1981 gccccgctca ccgtcgcggt cgccttggcc gacaagctcg acaccctcgt cggcttcttc
     2041 gccatcgacg aaaagcccac cggatcgaag gaccccttcg ccctgcgccg tgcggcgtta
     2101 ggtgtgatcc gcactttgct ggaaaacgaa gtccgtctgg aagtccgcga agccgtcagt
     2161 atggttcatt gggcccacgg caagcttggt ccgcattggc atcgcctact cgatgagggt
     2221 tcactcgagc ttgaggttca ggacttcttc gccgaccgcc tcaaagtcct cctccgcgac
     2281 caaggcaagc gccacgacct cgtcgacgct gtgttcgcgc tcggtgatga cgacctcgtc
     2341 cgcatcgtcc gccgcgtcga ggcgctcgac gccttcctcg ccaccgatga cggcgccaac
     2401 ctgctggccg ggtccaagcg cgccgccaac atcctgcgcg ccgaggccaa gaagggtgac
     2461 gtcccgacgg gcatggtcca gactggcctg ccgaaccagc ccgacgccga aaccgccctg
     2521 gccttcgccg tcgccagcgc ctcgaccgcc gtcgacgccg cgctggacac cgaggatttc
     2581 gccgccgcca tgaccgcgct cgcccgcctg cgcgccccgg tggacgcctt cttcaccgac
     2641 gtcatggtca actccgacgt ccccgaggaa cgcgccaacc gcctgaaact gctgggtcag
     2701 gtccacgacg tcatgggccg cgttgccgat ttcgcccaga tcgcgggctg atcggccgct
     2761 gacgcaaagc gtctcctccc cgtcgccgct tggcagtggg gagggggacc gcccgccgct
     2821 tggcgggtgg tggaggggct cttaaccgcg cacccgagct tgggtctccc gctggatcct
     2881 ttcgaggacc tcacttagat tgatgcgaac agcctcggcg ccgactctca gaacagcgat
     2941 cccttcccgc gccagccaat cggtacgtcg ctgatcgtgc agcattcgat ccggatgatc
     3001 atggctcatc ccgtcgacct cgacggccag cttcgcctga gcgcaataaa agtcgaggac
     3061 gtagcacccc actggatgct gccgccggaa cttgaatcct gcgagcgcct gacgtcgtag
     3121 ccgcgtccaa agccgggctt cgggtggagt cagggtccgc cgtgatacgc gtgctcgcga
     3181 tacagtgcct tgcgtggcca agagcccctc cgtcgcggcc gaagcgggcg ccgccacctc
     3241 cccatgctgc gcacagggag aagacgatga tcggcgttct ctggagtgcc ggtcaaggaa
     3301 tatattcctc atcatccgtt gcatacggtt cgtcccccac ccacttcatg ggtcggtctt
     3361 tgacatccgc gctcaatcaa tcaggcggtc acactggatt ggactccgag tccaatccag
     3421 ctccctgagg cggcgcttca tattcagtga gttgcaccac gctcctcact cgccaccttc
     3481 aattatggac tctctggact ctgttttttt ggagtccaac tagctcctga acaacagcga
     3541 cccgtcgccg tacgagtaga accggtaccc atccgcgatc gcgtgctcat acgccgaccg
     3601 catcgtctcc agtcccgcaa acgccgacac cagcatgaac agcgtcgact tcggcaggtg
     3661 gaaattggtc atcagcacat cgaccgcgcg gaaccggtaa ccaggcgtga tgaagatggc
     3721 cgtgtcgccg tggaagggct gaaccacgcc gtccggcgtc gtcgccgact ccagcagcct
     3781 cagcgatgtg gtcccgacgc agacgacccg gccgccgttc tcatgcaccg cgttcagagc
     3841 ggcggccgtc tcgggcgaca cctcacccca ctctgagtgc atcttgtggt cggcggtgtc
     3901 gtcggccttg accggcagga aggtcccggc cccgacgtgc agcgtcaccg catgggtcga
     3961 gacgcctctg gcgcggatgg cctccagcag ggcgggggtg aaatgcaacc ccgccgtcgg
     4021 cgcggcgacc gagccgtcat gctcggcgaa gaccgtctga tagtcggtca agtcgcgatc
     4081 gtcctcaggc cgcttggccg cgatataggg gggcaggggc atgaccccga cgtcgcggat
     4141 ggcgtcgtcc aacgccgggc cggccaggtt gaacgcgagg gtgatcaggc cgtcctcgga
     4201 cttggcggcg acggcggcgt ccagctgccc gagcatacag gcgttgtcca tgtcacgatc
     4261 gaaccggatg cggtccccga ctttcaagcg tttgccgggt ttcatgaagg cggaccagcg
     4321 atcaggggcg tcgcggtggt gcaaggtggc ttcaaccgca acggtcaggg tctggccggg
     4381 attctcggga tcgggccgtt ggcgcacacc cgacagccgc gccgggatca cgcgcgtgtc
     4441 gttgaacacc aaggcgtcgc cggggcgcag gaatgccggc aggtcgcgga tgacgcggta
     4501 gtccagaacg ccgtcctgca ccaccagcaa ccgggcggaa tcgcgcggct cggcaggacg
     4561 cagggcaatg tggtcttcgg gcaggtggaa gtcgaaatca gcggtcttca tcaggcggcg
     4621 caaaggccac ggacacaggg ttgggtcaag gcgtcaggag gcgccgcggt tgacgggggc
     4681 ggaaagtcca gaacgatttg cggttctgga tcccggaaca ctgccaatgg tgacgaagac
     4741 gatcagtgtc acggcgatgt taagaaccgc ctctagctga gggggaagta tggccccgcc
     4801 cagcacgctg cctgacaagc cctgcacgaa cccgaagccc aagccgaagc cgaacggaat
     4861 tagggcccag aaccatcttc tgtcgaaatc gtgcaagcgg cggcccgcgt taccagccat
     4921 aaaggcatcg atattgcgct gacgatcaga gccggtgtct ggaggccggc aagcacgatg
     4981 ttgccgacag ctagcagcac gaccgcaacc cagtattgct tccggtctga gcggcccttg
     5041 atgaacgcca ggacgcgatt ggatttttcg ccactcacac tgcttctcct caccatgtga
     5101 actgacaagc gctcaacatc aggttcacca cactgggcag gtcgacgccg tatggtcaag
     5161 ctgtccagag gcagaagcat gcctcacacc cgctgcctct cttcgtcgtc acggctgagg
     5221 ctgaacctcg gcacggcatg ggcgttcgag cgacatgaca aaccccggcc tgctcccttc
     5281 cgtcctcatc ggcgctgtcg ccagcgcgcg ttccatgacc ccgatggcgg cgatcgccgg
     5341 cgcgcggctg gcgcataggg acacaccggg caagctggtc ctgctggatc acccgctgtt
     5401 caggtacggc gctctggcca tgggtgtggg tgaactgttc ggcgacaaga tgaagtctgc
     5461 gcccgaccgc accgtcttcc tcggcctgct ggcgcgagtg ttgagcgcag ggatagccgg
     5521 cgcggctctg gccccgcgcg gtcgtgaaaa ggtcggtgcg gccctcgcca tcgggactgc
     5581 cgtgccgctg gcctatctcg gtctcgcggg tcgcaagcgg gctatggccg agatcggcca
     5641 gaccaaaagc ggcctgatcg aggacgccct gatcgtggct gcaggggccg ccgtcgtggc
     5701 ctgggtcgtc ggccgaaacc gctaacaccc ccgcaaacgt tgaccggggc gcattgacgg
     5761 gccaaacccc gcccatgctc cagaacctcg aaactctcgc ccgcgaggcc aagtcctggc
     5821 cgttcgaaca agcccgcaac ctgctggccc acgttctgaa aaagcggctg accgacgccg
     5881 agcgaactgc agccaagctg ttgatcgacg cgggcaaggc ggacgaggcg ctggcgacgt
     5941 tcgaggtgct gcagcgtccg gtgatcctcg aaaccggcta cggcccgtcc ggcctgccgc
     6001 acatgggcac gttcggcgag gtggcgcgca cgacgatggt caggaacgcc ttccgcgccc
     6061 tgaccggtga ccactggccg acgcgtctga tcgccttcag cgacgacatg gacggcctgc
     6121 gcaaagtgcc cgagggcgtg cccaatcccg agatgctgcg cgaggatctg cacctgtcgc
     6181 tgacccgcgt ccgcgatccg ttcggcacgc atgagagctt tggcgcgcac aacaacgccc
     6241 gtctgcgcgc cttcctcgac ggcttcggct tcgactacga gttcatgtcc tcgacccaga
     6301 cctacaaatc aggccggttc gacgcgacgt tgctgaccct gctggagcgg ttcgacgcca
     6361 tgatggcgat catgctgccg acgctgggcg aggagcgccg cgcgacctat tcgccgttcc
     6421 tgcccgttag cccgatcacc ggccatgtgc tgcaggtccc gacgctggcg cgcaatgtcg
     6481 agaaaggcac gatcaccttc gaggactccg caggcgagcc cggcaaccgc accgaagtcc
     6541 cggtcaccgg cggccatgtg aagctgcagt ggaagcccga ctgggccatg cgttgggtcg
     6601 cgcttgacgt cgattacgag atgtctggca aggacctgat cgagagcgtg cgcgagagcg
     6661 gcaagatctg tcgcgccttg ggcggtgtcc cgcccgaagg cttcaacttc gaactcttcc
     6721 tcgacatcga gggcaagaag atctccaagt ccaaaggcaa cggcctgact atggacgagt
     6781 ggctgcgcta cggcacgccg gagagcctga gctggtacat gttccagtcg ccgaagtcgg
     6841 ccaagtcgct gcacttcaat gtcatcccgc gcgccatcga cgactacctg agcttcatcg
     6901 aggcctacaa gacgcaggaa ccggccaagc agatcgacaa cgccgtctgg gcgatccacg
     6961 ccggatcgcc gccgtcgacc gtgtcgccgg tatcgttcgc gctgctgctc aatctggtcg
     7021 gcgtggccaa tgcctcgacc aaggatcagc tctgggccta tttcgccaaa tacctgccgg
     7081 aggcgacgcc ggacaatgag ccggtgctcg accggctgat gggccacgcg ctgaactact
     7141 acgaggactt cgtgaagccc tcgaagtcgt accgcctgcc ggacgataag gagaaggcgg
     7201 cgttgcttga tctggccggt cgtctcaaag ccttaccggc tgacagcacc gacggcgaag
     7261 tcatccagag cgaggtttac gcggtcggca aggagcatgg cttcgagccg ctacggggct
     7321 ggttcggcgc tctgtacgaa gttttgctcg gcgcttcgca aggtccccgt ttcggttcgt
     7381 tcgcggccat ctatggcctc ccacagacca tccagctgat cgaggacggc gcgaacgggc
     7441 agcttgtttg ataagattga gacgctatct caaaccttgg atgacttctc gccgactctc
     7501 cactatgcaa gcgtgagcat atggagcgtc ctgtttgcga cacacgatgg accaggacca
     7561 gcggatcttc catctcgacc tgaaccgatc cgactctgcc agggacgccc tcgaacagtc
     7621 gttgaccatt ctgggcaacc acccggaaat ctgggcctgg gattggattg ttgacgccca
     7681 gagggttcca gaagatgcct cggtcgagca gatcgcacga cttgcggagg tattcgcgac
     7741 cgtatccgcg acacggcctg tcgtcgtggc gacaacggct ttcgtcagtg ccgaccgcta
     7801 ccttcatctg tgggcgaggg tgatggatta ccagttcccc aagcgtaagc acctcgtcgt
     7861 gaccgatgtc gcccaggccg tgaaactgat cgcctcgcgt cgcgttggaa gatgaaccga
     7921 aaataacggc tacgctcggg gagccttcag gtacgatggc gcattctgcc tgatatcgac
     7981 caacctcgat gaactggaga gagccatgtc gaagacattg aacaccacga tcgccactgc
     8041 tgctgccttg gcagccctgc tcaccgccgc accgatggcg gcatccgccc aatcgaacgg
     8101 catcaccagc tgcgacgccc ccggcggtcg tcaacgcgca ggcgcggtga tcggcggcat
     8161 cctcggcggt gtcgttggta acaacctcgc ccgtaacgag cgcaccgcag gcaccatcgt
     8221 cggcgccggc gccggcgctg ccgccggttc ctacatcggc tgcaaccaac agcgcgcccg
     8281 ctcggcctcg gctcgcggtg agtatcgtgc gacgtcgaac ttgcgcatcc gttcgggccc
     8341 tggcacgaac tacggcaccc gcggcaccct gcaggccggc caaccgttca gcgtggtcag
     8401 ctcgtcgggc gaatgggtcc agatcgccgg cggcggttgg gtcaacgccc gctacgtcac
     8461 cacgaactag agttcgtcag acacaagatc aggggccgct ccgaaagggg cggcctttct
     8521 ttttgagccg tattcgcgcg acgcgcccgc tgcttgagcg ccaggttcag actgacgcac
     8581 cgaggcccca aggctggcta ctggctgacc caaaggatac gcgccatcca cacgacgtcg
     8641 cgtcggggca gggtccttgg tggataggcc gggtt
//
LOCUS       DASVTM010000535         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_156091,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000535 DASVTM010000000
VERSION     DASVTM010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156091"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1385)
                     /gene="astB"
                     /locus_tag="VF633_14795"
     CDS             complement(48..1385)
                     /gene="astB"
                     /locus_tag="VF633_14795"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEX8472377.1"
                     /translation="MTPGVEANADGLIGPTHSYAGLSPGNLAASLSKGEASNPRAAVL
                     QGLDKMKRLADLGLPQFVLAPHERPDLGFLRSLGFGGTDAQVLERAWKDAPAFLAAAT
                     SASPMWAANAATVTPSADSGDGRVHFTPANLHTNLHRSLEHRQTKRALDAMFPDPKHF
                     AVHDALPAVAQLSDEGAANHVRLCADHGAPGVNLLVWGREAHEPWSGAYPARQTLEAS
                     QAIARRHQAGRSVFPRQGKAAISGGTFHNDVVCVGALDTLLFHELAFEDTAGTKAAIR
                     AAAEGLFEPIFIEIAEADLPLADAIASYLFNSMLIQIPGEDRLTLICPTETRDNARAH
                     AVAQGLAASNGSIGRVEYVDVRQSMRNGGGPACLRLRVVLTDAELGATNPDMRITEGL
                     HGALSVWAMNWYRDRLTAADLADPALLDESRGALDELTGILGLGSGFYPFQRG"
     gene            1459..>1651
                     /locus_tag="VF633_14800"
     CDS             1459..>1651
                     /locus_tag="VF633_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472378.1"
                     /translation="MLAVWRRVALSWSSLRANRSSPFAFANGEVNAETPSIPQSLDLA
                     LDILHRTGLETRHRIGAVIR"
BASE COUNT          264 a          587 c          525 g          275 t
ORIGIN      
        1 cggttcggtc tttggctctg tcgcggtccg gtctgtcatc gtgcgtatca gcctcgttga
       61 aagggataga agcccgagcc gagccctaga atgccggtca gttcgtccag tgcgccccgg
      121 ctctcgtcca gcagggctgg gtccgccaga tcggcggcgg tcagccggtc gcgataccag
      181 ttcatcgccc agacgctcag cgccccatgc agcccctcgg tgatccgcat gtcggggttg
      241 gtcgccccca gttcggcatc ggtcagcacg acccgcaacc gcaggcaggc cgggccgccg
      301 ccgttacgca tcgactggcg cacatcgacg tattcgacgc gaccgatcga accgttcgat
      361 gccgccagtc cctgcgccac cgcatgggcg cgggcgttgt cgcgcgtctc ggtcggacag
      421 atcagcgtca gccgatcctc gcccggaatc tggatcagca tcgagttgaa cagataggat
      481 gcgatggcgt ccgccagcgg caggtcggcc tcggcgattt cgatgaagat cggttcgaac
      541 agcccctcgg cagcggcgcg gatcgcggcc ttggtccccg ccgtgtcctc gaacgccagt
      601 tcgtggaaca gcagcgtgtc caatgctccg acgcagacga catcgttgtg gaaggtcccg
      661 cccgagatcg ccgccttgcc ctgacgtggg aacaccgaac gccccgcctg atgccgccgc
      721 gcgatggcct gcgacgcctc cagcgtctgg cgcgcaggat aggctccgct ccacggctca
      781 tgggcctcac gtccccagac cagcagattg acccccggcg cgccatggtc ggcgcacagc
      841 cgcacgtggt tcgccgcgcc ctcatcggac agctgggcga cggcgggcag ggcgtcgtga
      901 accgcgaaat gcttcggatc ggggaacatc gcgtccagcg cccgcttggt ctgccgatgc
      961 tcgagcgacc ggtgcagatt ggtgtgcaga ttggccggag tgaaatgaac ccgcccgtcg
     1021 ccactatcag cgctcggcgt caccgtcgcc gcattggccg cccacatcgg cgaggccgaa
     1081 gtcgccgccg ccaggaacgc cggtgcatcc ttccacgcgc gttccagcac ctgcgcatcc
     1141 gtgccgccga acccaaggct cctcaggaaa ccgaggtccg gtcgctcatg cggcgccagc
     1201 acgaactgcg gcagaccgag gtccgccagc cgcttcatct tgtcgagccc ctgcaggaca
     1261 gcggccctcg ggttcgacgc ctcgcccttg ctgaggctgg ccgcaaggtt gccgggcgac
     1321 agccctgcat atgaatgcgt cggcccgatc aagccgtcgg cattggcttc gacgccgggg
     1381 gtcatgggcg gtcacctccc cattcgcgaa ggcgaatggg gaggacagac tgcgaagcag
     1441 tcaggtgggg gcgacaccgt gctggccgtc tggcgccgcg tagccctctc ctggtcgtcg
     1501 ctccgcgcca accggtcctc tccattcgcc ttcgcgaatg gagaggtgaa cgctgagacg
     1561 ccgtcaattc cgcaatccct tgatctcgcc ctcgatattc ttcaccgcac cggcctcgaa
     1621 actcgccacc ggataggcgc agtaatccgc c
//
LOCUS       DASVTM010000536         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_1007648,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000536 DASVTM010000000
VERSION     DASVTM010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007648"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF633_14805"
     CDS             complement(<1..450)
                     /locus_tag="VF633_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEX8472379.1"
                     /translation="MVATDDTYTDSLLVPGQTVWRVEQADRFAVLMENETYFDALASA
                     ISKAQRSIVLLGWQFDPRTRLDPGSRAAGRQAEIGHQLRMLVRSRPELDVRLLIWKSP
                     LLIAASQGFYPHKAQRWFRKRMVEFRLDSPGPIGACHHQKVVVIDDKV"
     gene            654..836
                     /locus_tag="VF633_14810"
     CDS             654..836
                     /locus_tag="VF633_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8472380.1"
                     /translation="MQALLELIAAFIAMLAMAALSQFGVDLHRPSMPEREVHRVIDCR
                     EASARTEVISTSAERC"
     gene            976..>2097
                     /locus_tag="VF633_14815"
     CDS             976..>2097
                     /locus_tag="VF633_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tipN"
                     /protein_id="HEX8472381.1"
                     /translation="MKSRQRPPLTLSDPDIVDPATVSDEHIASDSFDAPSADQPTTDV
                     DDPLVVAPAMPERPMSERRRRRLLDEQRQAEARAAARVETQAARAKVAEDVLGPLPDS
                     APQAEPVVTPDFNGSQSAYDPFTTSAESPPVAYRSPPARTAETPVSAGRPAKTASRVG
                     EAVPSGRHAYLLAGVASALWIGGVASWAAYEIGSGGAALDPLRLALYALIALAPAGLA
                     VMLAHAIRQGAGLAAETVRARALAEALVAPTALAAQQTGQVLLTLRNDIDQAALAAER
                     ARNDMTLLREVLVEETTRLNEAAENAGRTARRLTEQLGRERDSMQVVGLQLDTQSAGV
                     LDAVERQSRMIVDASDLAQTQLREAEAALAARAADLAAAA"
BASE COUNT          338 a          728 c          679 g          352 t
ORIGIN      
        1 gaccttgtcg tcgatcacca caaccttctg gtgatggcag gcgccgatcg gcccgggtga
       61 atcgaggcgg aactcgacca tgcgcttgcg gaaccaacgc tgggccttgt gcggataaaa
      121 gccctgcgac gctgcaatca acagcggcga tttccagatc agcagcctga cgtcgagttc
      181 gggcctggag cgcaccagca tccgcagttg atgcccgatc tcggcctgcc gccccgccgc
      241 ccggctcccc gggtcgaggc gggtgcgggg gtcgaattgc cagcccagca gcacgataga
      301 ccgttgcgcc ttggaaatag ccgaggccaa ggcgtcgaaa taggtctcgt tttccatcag
      361 gacggcgaac cggtcggctt gctccacgcg ccagaccgtc tggccgggga ccagcaggct
      421 gtcggtgtag gtgtcgtccg ttgcaaccat gaatcttgtc ttggggggat acgcgtgaca
      481 gctgggtttc aggaggcgac aagcccactg gaaggacctg cctgtgtctt ccgttccatg
      541 ccggccgggg atcggtcaat ggccacgctc ctgttgcggg ccaatgcttg tccgaacgct
      601 gattttggcc catacaagcc tgactgtcgc aggacgaagg acgggagggt gaaatgcagg
      661 ctttgctcga actgatcgcc gccttcatcg cgatgctcgc catggccgcc ctgtcccagt
      721 tcggcgtcga tctgcatcgc cccagtatgc ccgagcgcga agttcatcgc gtcatcgatt
      781 gccgcgaagc ctcggcacgc accgaggtca tctctacctc cgcggaacgc tgctgatccc
      841 aggggtggct ggtcgggtcc gggtgattca ctatgggcag cggactcgtc tttgcggacg
      901 cgtccttagt ggttagggtt gcgaagcctg ttgcgccgag cccggcccca gactcccaga
      961 ccgagacctc gcctgatgaa atcccgccag cggccgccgc tcacactgtc cgaccccgac
     1021 atcgtggacc ccgcgaccgt gtccgacgaa cacatcgcat cggatagttt cgacgcgccg
     1081 agcgccgatc aaccgacgac ggacgtcgat gatccattgg tcgtcgcccc ggcgatgccc
     1141 gaacgcccga tgtcggagcg tcgtcggcgg cgtctgctgg acgagcaacg tcaggctgaa
     1201 gcacgggcgg ccgcccgggt tgaaacccag gccgctcgcg ccaaggtcgc cgaggatgtg
     1261 ctcggccctc tgccggactc tgcaccgcaa gcggagccgg tcgtgacgcc ggacttcaac
     1321 ggttcccagt ccgcctatga tcccttcacg acgagcgccg aaagcccgcc ggtggcctat
     1381 cgctcgcctc cggccaggac ggcagagacc ccggtttccg ccggccggcc cgcaaagacc
     1441 gcctcacggg tgggcgaagc agtcccgtct ggccgccacg cctatctgct ggccggcgtg
     1501 gcgtcagccc tctggatcgg cggcgtcgcc tcatgggctg cctatgagat cggttcgggc
     1561 ggagcggccc tcgatcccct gcgccttgct ctctacgccc tgattgcact cgcgcctgca
     1621 ggcctcgccg tcatgctggc ccatgccatc cgtcaggggg ctggtctggc agccgagact
     1681 gtccgcgccc gcgctctggc ggaggccctc gtagccccga ccgctctggc cgcccagcag
     1741 accggacagg tcctgctgac gctgcgcaac gacatcgatc aggcggcttt ggcggcagag
     1801 cgcgcccgca acgacatgac cctgctgcgc gaggtgctgg tcgaggaaac cacccgcctg
     1861 aacgaggcgg ctgaaaatgc gggccgcacc gctcgtcgcc tgaccgagca actcggccgc
     1921 gaacgcgaca gcatgcaggt ggtcggcctg cagctcgaca cccaatccgc cggcgttctg
     1981 gatgcggtcg agcgccagtc gcgtatgatc gtggacgcct cggatctcgc gcagacccag
     2041 ttgcgggaag ccgaagccgc cctcgcggcg cgcgccgcag acctcgctgc cgcggcc
//
LOCUS       DASVTM010000537         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_157049,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000537 DASVTM010000000
VERSION     DASVTM010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157049"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VF633_14820"
     CDS             <1..970
                     /locus_tag="VF633_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8472382.1"
                     /translation="PPLIRLERANVGYDTGKPILRNLNLRMDLDDRIGLLGVNGAGKS
                     TFAKMIAGALNVSEGELHRDRKMRVGWFHQHQIEAMDPTDTPLEIIRRAMPDSAESAR
                     RSKLAQFGLSYEKQETTVDSLSGGERARLLLNMVAMDAPHVLILDEPTNHLDIDSRRA
                     LLDALNEYSGAVILITHDRSLMEMVADRLWLAADGTVKPFDGDMDDYAKFVLDRAKQA
                     SVKPSQVKTEAVAAAPAQNNKNKKNDKKSGPSPSTLRHAVKKAEERMAQLTAELARID
                     DDMANAAFSNPKALEGLTRARAKTQVDLDAAEAAWVAAEEALAEVS"
     gene            970..1221
                     /locus_tag="VF633_14825"
     CDS             970..1221
                     /locus_tag="VF633_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HEX8472383.1"
                     /translation="MKPADPIEFSKDEMRDMIRRVQAYFDAELEQDIGALPAEMLIAF
                     FGREVGGFFYNRGVRDAQQLIQQKTEDIVEALYGLERSR"
     gene            complement(1283..1981)
                     /locus_tag="VF633_14830"
     CDS             complement(1283..1981)
                     /locus_tag="VF633_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEX8472384.1"
                     /translation="MPVKTVAEFVAAYREKDVSTEAFELENQYLLEIRLNGSVWAKSR
                     AMVARTGQVKFTRQGLMEQGLGNLLKKAVSGEGMVLMKAEGQGRVYLADAGKKITLLR
                     LDNETIFANGNDVLALENGVSNQITMMKRVAGMMSGGLFNVKLSGSGIVAITSHYDPL
                     ILKVSAATGPVFTDPNATVAWSGSLMPQIATDISFKTLVGRGSGESIQMKFEGEGWVV
                     VQPYEETYMQAASN"
     gene            2234..>3598
                     /locus_tag="VF633_14835"
     CDS             2234..>3598
                     /locus_tag="VF633_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX8472385.1"
                     /translation="MSRLLLSACAAALLSAAPALAQQTFPQTGTPIIADSTQAALALP
                     GPQAAIPVPQDKPYPGVIRYRADVTDLERRIVNVTETIPVSPGALTLLYPQYLPGNHA
                     ATGPIQLISGVTVTGNGARIEWVRDTLQPYAFHLNIPEGVTEIEVAFQWLTQPDNAVW
                     RVTMTPAIVNMQWEKALLYPAGYYSRQITVAPSIKLPEGWQYGTALETERFADGVATF
                     KPTDLYTLIDSPMFAGSHYRRIDIDPTGDAVHLNIVADKAANLAPTADQLKLYENLVG
                     QTDRLFGARHYDRYEFLLALTDQLGGIGLEHHRSSENTAAPDYFTAWDKSVGGRELLP
                     HEFTHSWNGKFMRPADELTANYNVPTQNTLLWVYEGQTEYWGSVLSARSGLHTKEEAL
                     IDLAGVAAFYENQPGREWRALQDTDNHNLLGYRVPGLWTSWMRGTGDYYRESQLMWLD
                     ADTLI"
BASE COUNT          676 a         1257 c         1059 g          606 t
ORIGIN      
        1 gccgccgctg atccggctgg agcgcgccaa cgtcggctac gacaccggca agccgatcct
       61 gcgcaatctc aacctgcgca tggacctcga cgaccggatc ggcctgctgg gcgtcaacgg
      121 cgcgggtaaa tccaccttcg ccaagatgat cgccggcgcc ctgaacgtct ccgaaggcga
      181 actgcaccgc gaccgcaaga tgcgcgtggg ctggttccac cagcaccaga tcgaggcgat
      241 ggaccccacc gacacgccgc tggagatcat tcgccgcgcc atgcccgaca gcgcggaaag
      301 cgcccgccgc tccaagctgg cgcagttcgg cctcagctac gaaaagcagg aaaccacggt
      361 cgacagccta tccggcgggg agcgcgcgcg cctgctgctc aacatggtgg ccatggacgc
      421 cccccacgtc ctgatcctcg acgaaccgac caaccacctg gacatcgaca gccgccgcgc
      481 cctgctggac gcgttgaacg aatactccgg cgccgtcatc ctgatcaccc acgaccgctc
      541 gctgatggag atggtcgccg accggctgtg gctggctgcc gacggcacgg tgaagccgtt
      601 cgacggcgac atggacgact acgccaaatt cgtcctcgac cgcgccaaac aggcctcggt
      661 caagccgagc caggtcaaga ccgaagccgt cgctgcggct cccgctcaga acaacaagaa
      721 caagaagaac gacaagaaat caggtccttc cccctcgacc ctgcgtcacg cggtgaagaa
      781 ggccgaagag cggatggccc agttgaccgc cgaactcgcc cgcatcgacg acgacatggc
      841 caacgccgcc ttcagcaacc ccaaagccct cgaaggcctg acccgagcgc gagctaaaac
      901 gcaggttgat ctcgacgccg ccgaagctgc gtgggtcgcg gcggaagaag cactggctga
      961 agtgagctga tgaaacccgc cgaccccatc gagttctcga aagacgagat gcgcgacatg
     1021 atccggcggg tgcaggcgta tttcgacgcc gagctagagc aggacatcgg agccctgccc
     1081 gccgagatgc tgatcgcctt cttcggtcgc gaagtcggcg gcttcttcta caatcgcggc
     1141 gtccgcgacg cccagcagtt gatccagcag aaaaccgaag acatcgtcga ggctctctac
     1201 ggacttgagc ggtcccggta gcgatcatta accgaaggcg gtgggcggca gcgggcgggg
     1261 gccctccccg ccctcaccgt cgtcagttac tagcggcctg catatacgtt tcctcgtagg
     1321 gctggacgac gacccagccc tcgccttcga acttcatctg gatgctctcg cccgaacctc
     1381 ggccgacgag ggtcttgaac gagatgtcgg tagcgatctg cggcatcagg ctgcctgacc
     1441 aagccacggt ggcgttggga tcggtaaaga ccgggccggt cgccgcgctg actttgagga
     1501 tcagcggatc atagtgggat gtaatggcga cgatgccgct tccgctcaac tttacattga
     1561 acaggccgcc ggacatcatg cccgcgaccc gcttcatcat cgtgatctgg ttactgacgc
     1621 cgttctccag cgccagcacg tcgttgccgt tggcgaaaat ggtctcgttg tcgagccgca
     1681 gcagggtgat cttcttgccc gcgtcggcca aatagacacg tccctgaccc tccgccttca
     1741 tcagcaccat gccttcgccg ctaacggcct tcttcaacag attgccgaga ccctgctcca
     1801 tcaggccctg acgcgtgaac ttcacctgcc ccgttcgcgc caccatggcc ctggacttgg
     1861 cccagaccga gccgttcagc cgtatctcca gcagatactg gttctccagt tcgaaggctt
     1921 cggtgctgac gtccttctcc ctgtaggcgg cgacgaattc ggcgacggtt ttcacgggca
     1981 tggggaacct caaggacaaa gacgggaagg cgagcttggg cgaacaaccc acgaccccgc
     2041 aagtttatct gagtgcgagg gttcgcgcct tggctggttc atgccgcaac ccctcaccct
     2101 ttcgcctgac caatcactgc gctcccggag gctcaagccc tctcccgccg ggagagggtg
     2161 gcatcttggc gggttgacgt aggggcgggg gcagtcgagg tttcgacctc ctgctgttcg
     2221 agaacccctg tccatgtcgc gtctgcttct gtccgcctgt gcggccgctc tcctgtccgc
     2281 cgcccccgct ctcgcccagc agaccttccc ccagaccggc acgcccatca tcgccgattc
     2341 gacacaggcg gcgctcgccc tgcccggccc acaagcggcc attcccgtcc cgcaggacaa
     2401 gccctatccc ggcgtgatcc gttaccgcgc cgatgtcacc gacctcgagc gccggatcgt
     2461 caacgtcact gagaccattc ccgtctcgcc cggcgccctg accctgctct atccgcaata
     2521 tctgcccggc aaccacgcgg cgaccggccc gatccaattg atctccggcg tgaccgtgac
     2581 cggcaatggc gctcgcatcg agtgggtgcg cgatacgctg cagccctatg ccttccacct
     2641 gaacatcccc gagggcgtga ccgagatcga ggtcgccttc cagtggctga cccagcccga
     2701 caacgccgtc tggcgcgtca ccatgacccc ggccatcgtc aacatgcagt gggagaaggc
     2761 cctgctctat ccagcgggct actattcgcg tcagatcacc gtcgccccgt cgatcaagct
     2821 gcctgagggt tggcagtacg gcacggcgct ggagaccgag cgcttcgccg acggtgtcgc
     2881 caccttcaag ccgaccgatc tctacaccct gatcgacagc ccgatgttcg cggggtccca
     2941 ctatcgccgg atcgacatcg acccgaccgg cgacgctgtg catctgaaca tcgtcgccga
     3001 caaggccgcg aacctcgcgc ccaccgccga ccagctgaag ctgtatgaga accttgtcgg
     3061 ccagacggac cgcctgttcg gcgctcgcca ttatgaccgg tacgagttcc tgctggccct
     3121 gacggatcag ctcggcggca tcgggctcga gcatcatcgc tcgtccgaga acaccgccgc
     3181 accggactat ttcacggcct gggacaagag cgtgggtggt cgcgagctgc tgccgcatga
     3241 gttcacccac tcgtggaatg gcaagttcat gcggccggcc gatgagctga ccgccaacta
     3301 caacgtgccg acccagaaca ccctgctgtg ggtctatgaa ggccagacgg agtactgggg
     3361 ctcggtgctg tccgcgcgct ccggcctgca tacgaaggaa gaagcgctga tcgatctcgc
     3421 gggcgtcgcg gccttctacg aaaaccagcc gggccgtgag tggcgcgcgc tgcaggatac
     3481 cgacaaccat aatctgctcg gctatcgcgt gccgggccta tggacgtcgt ggatgcgcgg
     3541 caccggtgac tactaccgcg agagccagct gatgtggctc gacgccgaca ccctgatc
//
LOCUS       DASVTM010000538         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_424309,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000538 DASVTM010000000
VERSION     DASVTM010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424309"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..616
                     /locus_tag="VF633_14840"
     CDS             302..616
                     /locus_tag="VF633_14840"
                     /inference="COORDINATES: protein motif:HMM:NF021496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HEX8472386.1"
                     /translation="MPAPSLVQTALVQTAHAETAHASRYLQQLAKHWGHKFPVTFTPD
                     TATIDLPMGRCELAADATALDVTLNGSPDADMERFKTVVAEHLQRFGHRETLIFDWRD
                     PA"
     gene            728..1696
                     /locus_tag="VF633_14845"
     CDS             728..1696
                     /locus_tag="VF633_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8472387.1"
                     /translation="MTPTTYTPPSAAPVITIEGLTKTYKSGLQALKQVDLSIGKGEIF
                     ALLGPNGAGKTTLISILCGIVTPSTGKVVADGHDIQNDYRRARSKIGLVPQELTTDAF
                     ETVLATVTCSRGLFGKSPNPAFIEQVLRDLSLWDKKDAKIMTLSGGMKRRVMIAKALS
                     HEPDILFLDEPTAGVDVELRRDMWLLVRKLRDRGVTIILTTHYIEEAEEMADRVGVIL
                     KGELILVEETKTLMKKLGKKTLTLNLIEAMPVIPAELADWKLELRNEGHELEYSFDAN
                     AEDTGVPSLIRALEKLGVGFKDLNTKQSSLEDIFVSLVHREKEPAQ"
     gene            1693..2460
                     /locus_tag="VF633_14850"
     CDS             1693..2460
                     /locus_tag="VF633_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8472388.1"
                     /translation="MTFNGYGVWAIYRFEMARALRTLWQSVVTPVITTALYFVVFGSA
                     IGGRMTEVDGVPYGAFIVPGLIMLSLFTQSIFNASFGIYFPKFTGTIYEILSAPVSSM
                     EIVIAYVGAAATKSAVLGLIILLTASFFVPLQILHPFAMFAMLVLISVTFSLFGFIIG
                     VWANGFEQLQMIPMLVVTPLTFLGGSFYSIDMLPPVWRTITLFNPVVYLISGFRWTFY
                     GSGDVGVGYSVLATLGFLVVCLGVVAWIFKTGYRLKN"
BASE COUNT          480 a          856 c          747 g          485 t
ORIGIN      
        1 gccaaccgct ggtgcgattt tcaccgtggg gaacagcgct ccattcggcg ccggcaaggt
       61 cggattgggg ccgaatcctg ccgcaggatc cagcggcttc tgggagctgc aggcagccag
      121 cgtcaggaca gcagctgaaa cggtgaggaa taagcgagac attttggctc cagagtgcgg
      181 tgacaacata cagctacggc cccggttcct caaagacgca gctagagcga ctgagtaccg
      241 ctccgcaacg ttgcgaaacg ctcgcaagag cctatctgta tgccttgccg aaaggatcgc
      301 catgcctgcc ccatcccttg tccaaaccgc cctcgttcaa accgcccacg ccgaaactgc
      361 ccacgccagc cgctaccttc agcaattggc caaacactgg ggccacaagt ttcccgtcac
      421 attcactccc gacacggcga ccatcgatct ccccatgggc cgatgcgaac tcgcagctga
      481 cgccaccgcc ctcgacgtta cgctcaacgg ttctcccgac gccgacatgg aacggttcaa
      541 gacggtcgtc gccgaacacc tgcagcgctt cggccatcgc gagaccctga tcttcgactg
      601 gcgcgacccg gcctgagagc gatccggcgc cgaaatctgt cagcatcttt cccgcacgcc
      661 ttgagacccg gccgaacagc gccatattgg ccagcgcggc cgtagcgacg gcgcgcgggg
      721 agagttcatg acgccgacca cctatacccc gccgtctgcg gcgcctgtca tcacgatcga
      781 aggcctgacc aagacctaca agtccgggct ccaagccctg aagcaggtcg atctcagcat
      841 cggcaagggc gagattttcg ccctgctggg ccctaacggc gccggcaaga ccaccctgat
      901 ctccatcctc tgcggcattg tcacgccctc gacgggcaag gtcgtcgccg acggccacga
      961 catccagaac gactatcgcc gcgcccgctc gaagatcggg ctggtgccgc aagagctgac
     1021 caccgacgcc ttcgagacag ttctggcgac cgttacctgc agccggggcc tgttcggcaa
     1081 gtcgccgaac cctgccttca tcgagcaggt gctgcgtgat ctgtccctgt gggacaagaa
     1141 ggatgccaag atcatgaccc tgtccggcgg catgaagcgc cgggttatga tcgccaaggc
     1201 cctgagccac gagcctgaca tcctgttcct cgatgagcca acggcgggcg tcgacgtcga
     1261 actgcgccgc gacatgtggc tgctggtcag aaagctgcgc gatcgcggcg tgaccatcat
     1321 cctgacgacc cactacatcg aggaggccga ggagatggcc gaccgggtgg gcgtgatcct
     1381 gaagggcgaa ttgatcctgg ttgaagagac caagaccctg atgaagaagc tcggcaagaa
     1441 gacgctgacg ctcaacctga tcgaggccat gcccgtcatc cccgccgaac tggccgactg
     1501 gaagctggaa ctgcggaacg agggccacga gctggaatac agcttcgacg ccaatgccga
     1561 ggacaccggc gtgccgtccc ttatccgtgc gctggagaaa ctgggcgtcg gcttcaagga
     1621 tctgaacacc aagcaaagct cgctcgagga catcttcgtc agccttgtcc accgcgagaa
     1681 ggagccagcc caatgacgtt caacggttac ggcgtctggg ccatctatcg tttcgagatg
     1741 gcgcgcgccc tgcgcaccct gtggcagtcg gtagtcaccc cggtcatcac aacggcgctc
     1801 tatttcgtgg tcttcggctc tgccatcggc gggcggatga ccgaggtcga cggcgtgccc
     1861 tacggcgcct tcatcgtacc tggcctgatc atgctgagcc tgttcaccca gtcgatcttc
     1921 aacgcctcgt tcggcatcta cttccccaaa ttcaccggca cgatttacga gatcctgtct
     1981 gcgccggtca gttcgatgga gatcgtcatc gcctatgtcg gggcggcggc gaccaagtct
     2041 gcggtgctcg gcctcatcat cctgctgacg gcgtcgttct tcgtgccgct gcagatcctc
     2101 caccccttcg ccatgttcgc gatgctggtg ctgatctcgg tgaccttcag cctgttcggc
     2161 ttcatcattg gcgtctgggc caacggcttc gagcagctgc agatgatccc gatgctggtc
     2221 gtgacgcctc tgaccttcct cggcgggtcg ttctattcga tcgacatgct gccgcccgtc
     2281 tggcggacca tcaccctgtt caacccggtg gtctatctga tctcgggctt ccgctggacc
     2341 ttctacggct cgggcgacgt cggcgtgggc tacagcgtcc tggccacgct cggctttctc
     2401 gtggtctgcc tcggcgtcgt cgcctggatc ttcaagacgg gctaccggct caagaactga
     2461 cctgcccggt cttaatcctt ggcctgaacg tgtcgtaagg tcgcgtcacg aaacggtcga
     2521 actgagccgc catcgtcgcg ttcagtatat cgcgcgtggg gaagagcc
//
LOCUS       DASVTM010000539         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Brevundimonas sp. isolate SMAG_U3291 k141_425289,
            whole genome shotgun sequence.
ACCESSION   DASVTM010000539 DASVTM010000000
VERSION     DASVTM010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959911
            Sequence Read Archive: SRR10954965
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Brevundimonas sp. (soil metagenome)
  ORGANISM  Brevundimonas sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:38:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 9 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Brevundimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425289"
                     /isolate="SMAG_U3291"
                     /isolation_source="soil"
                     /db_xref="taxon:1871086"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VF633_14855"
     CDS             <1..917
                     /locus_tag="VF633_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8472389.1"
                     /translation="DRAASDFFAPADSPEGRWNARMDAISGALNGAKFAEVSTLDYDA
                     YRDTGVNYRVVEGYGALIGALGGDLPVVLDCAVKRIDRTGPAMKVATVRGDVLAKVVV
                     LTVPTNLIAAETIRFDPPLPETVEAAAGLPLGLAAKLHMTVETPEDFPADSQLWGRTD
                     TAQTGGYHLRPFGRPTIEGYYGGDLAGGLEAEGESAFFDFAVSELVAQLGSDFRKRLA
                     VLNTSMWGAEPWSRGAYSHALPGHAGDRSRLKHPAESRLFIAGEATAPAFYGTCHGAW
                     IEGERAALEAMTKLGVDRWLSEPREEER"
     gene            977..1669
                     /gene="nth"
                     /locus_tag="VF633_14860"
     CDS             977..1669
                     /gene="nth"
                     /locus_tag="VF633_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX8472390.1"
                     /translation="MTGAVIPTWGWPPDEDRVEDIFERLSRVMPEPKTELTFSSPFTL
                     VVAVALSAQATDVSVNKATDKLFKVADTPAKMLALGEEGLVPYIASIGLYRGKAKNVI
                     ALSRIVQDQYGGEVPLTREGLQALPGVGRKTASVVLNELGIEAAIAVDTHVFRVSHRL
                     GLANAPTPDKVEAQLFKVVPEAFLPKAHHWLILHGRYTCTARKPNCMGCIISDLCPSR
                     AGLVAMGEARQA"
     gene            complement(1660..>1992)
                     /locus_tag="VF633_14865"
     CDS             complement(1660..>1992)
                     /locus_tag="VF633_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX8472391.1"
                     /translation="TEGETSLLFFGGKFSHAVNKTPVAGEFRIQVQFGGQYVSVEPPA
                     AAMELALKVLAAIEEPLLYARIDMTRDQAGAWVLMEAELIEPDFYLANDRRKGAGFAE
                     AVRARLQA"
BASE COUNT          330 a          685 c          661 g          316 t
ORIGIN      
        1 aggaccgggc tgcatccgac ttcttcgccc ccgccgacag tcccgagggt cgttggaacg
       61 cgcggatgga cgccatctcc ggcgctctga acggagccaa attcgcagag gtctcgacac
      121 tcgactacga cgcctatcgc gacaccggtg tgaactatcg ggtggtcgag ggctatggcg
      181 cactgatcgg ggcgctgggc ggcgaccttc cggtggttct ggactgcgcc gtcaaacgca
      241 tcgaccgaac tgggcctgcc atgaaggtcg cgacggtgcg cggggatgtg ctggcgaagg
      301 tcgtcgtcct gacggtgccg acaaacctga tcgcggccga gaccatccgc ttcgatccgc
      361 cgcttcctga gacggtcgag gcggcggcag gcctgccgct ggggctggcg gccaagctcc
      421 atatgacggt cgagacgccc gaagacttcc ccgccgacag ccagttgtgg ggccggaccg
      481 ataccgccca gacgggcggc tatcacctgc gcccgttcgg acggccgacc atcgagggct
      541 attacggcgg cgatctcgcc ggggggctgg aggccgaggg cgaaagcgcc ttcttcgatt
      601 tcgccgtgtc ggaactggtc gctcagctgg ggtcggattt ccgcaagcgg ttggcggtgc
      661 tgaacacctc catgtggggg gccgaaccct ggtcgcgggg agcctattcc cacgccctgc
      721 ccggccatgc gggcgaccgc tctcggctga agcatcccgc ggaaagccga ctgttcatcg
      781 ccggcgaagc aaccgcgccc gccttttacg gcacctgtca cggcgcatgg atcgagggcg
      841 agcgggctgc actggaagcc atgacgaagc tcggcgtcga ccgctggctg tcggagcccc
      901 gggaagagga acggtgaaag cgccggtccg gagaaaagcc aaggcgcccc tgcccgcccc
      961 gaaacgcccg tcgatcatga ccggtgcagt catcccgacc tggggctggc cgccggacga
     1021 ggaccgggtc gaggacatct tcgaacgcct ctcgcgcgtc atgccggagc cgaagaccga
     1081 gctgacgttt tccagcccgt ttacgcttgt ggtcgccgtc gccctgtcgg cgcaggccac
     1141 cgacgtctcc gtcaacaagg ccaccgacaa gctgttcaag gtcgccgaca ccccggcgaa
     1201 gatgctggcg ctcggcgagg agggactggt cccctacatc gcctccatcg gcctgtatcg
     1261 cggcaaggcg aagaacgtca tcgctctgag ccgcatcgtt caggaccagt acggcggcga
     1321 ggttcctctg acccgcgagg ggctgcaggc cctgcccggc gtggggcgaa agaccgccag
     1381 cgttgtcctg aacgaactcg gcatcgaggc cgccatcgcc gtggacaccc acgtgtttcg
     1441 cgtctcgcac cgcctcggcc tcgccaacgc ccctacgccc gataaggtgg aggcccagct
     1501 gttcaaggtc gtccccgagg ccttcctgcc caaggcccac cactggctga tcctgcacgg
     1561 ccgctacacc tgcacggcgc gcaagccaaa ctgcatgggc tgcatcatca gcgacctgtg
     1621 cccgtcgcgc gccggtctgg tggcgatggg cgaagcgcgt caggcctgaa gccgcgcccg
     1681 gaccgcctcg gcgaagcctg cgcccttcct ccggtcgttg gccagataga agtccggctc
     1741 gatcagttcg gcctccatca ggacccaggc gccggcctga tcgcgcgtca tgtcgatgcg
     1801 ggcgtagagc agcggctcct cgatcgcggc gaggaccttc agcgccagtt ccatcgcggc
     1861 ggcgggcggc tcgactgaga cgtactgacc cccgaactga acctgaatgc ggaattcgcc
     1921 cgcgaccggc gtcttgttga ccgcatggct gaacttgccg ccaaagaaca gcaggctggt
     1981 ttcaccctcg gt
//
